LOCUS       DASVIM010000001         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_426563, whole genome shotgun sequence.
ACCESSION   DASVIM010000001 DASVIM010000000
VERSION     DASVIM010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426563"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1249
                     /gene="mltG"
                     /locus_tag="VF305_00005"
     CDS             113..1249
                     /gene="mltG"
                     /locus_tag="VF305_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX7415551.1"
                     /translation="MKELKKKKTNKKPGRITGLIGGDKRRAGVRRMQFPSFKFFKMPR
                     FVGRAIHYVILAAAVAIVLFGVLLINYATNSIDNEKNITVLVEIPTGSSFLKITKILN
                     QAGLVKSRFFFYSLAMTKRATRSIRAGEYEFNTSLTPGAVIDKLIRGEIKKYLVTIPE
                     DFSIKEIAIRLAYSKLIDEETFFELARNKNFLESLGIKADSIEGYLFPDTYLFDRSMS
                     TRQIMKIMVSQFWKKVTPEMLKMAGELGFTPHQFVTFASIVGKESGDNAEKPMISAVF
                     HNRLKKKMRLQSDPTAVYDLDNFDGQVLRKHLKRNSPYNTYIIRGLPPGPIANPGLTS
                     LRAALYPASVNYLYFVSKKDGSHFFSDSLDMHNQAINRYRIRKK"
     gene            1512..>1902
                     /gene="mraZ"
                     /locus_tag="VF305_00010"
     CDS             1512..>1902
                     /gene="mraZ"
                     /locus_tag="VF305_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX7415552.1"
                     /translation="MSVFRGQYHHNIDEKGRIIFPSKFREVFGEKYDNRMVITNWDGY
                     LMVFPYDEWRIIEEKVSHQSILRKEVRAFQRFFMSGAVDCNLDGQGRILIPPALRDYA
                     QLEKDIILAGMIKVIEIWSKERFEDEMK"
BASE COUNT          589 a          350 c          377 g          586 t
ORIGIN      
        1 ttcgctggaa aaaaagaaaa gataaggtgg ataaactggc cgcttgcctc attcttcagg
       61 gttatttgga ttccgtcaca aaataatttt gaaaattaat tccacgaaaa ttatgaaaga
      121 attgaagaaa aagaagacta ataaaaaacc cggcagaata accggtctta taggcgggga
      181 taaacgccga gcgggagtaa ggaggatgca atttccctcg tttaaatttt ttaagatgcc
      241 ccgctttgta ggcagggcaa ttcactacgt cattctagct gctgcagttg ctattgtcct
      301 ctttggtgtt cttttaataa attatgcaac taattccatt gataatgaga aaaatataac
      361 tgtgctggtg gagataccca ccggctccag cttcctaaaa attacaaaaa tactgaatca
      421 ggcgggactg gtcaagagcc gctttttctt ctatagctta gcaatgacaa aaagagctac
      481 ccgttccatc cgggccggtg aatatgagtt taacacttcc ctgactcctg gcgcagtaat
      541 tgacaaattg atccgtggag aaatcaaaaa atatctcgtc actattcccg aagatttttc
      601 catcaaggaa atcgccatac gcttagctta ttctaagcta attgacgaag aaactttttt
      661 tgaactggca agaaataaaa attttttaga atctttgggt attaaggccg attctataga
      721 aggttacctt tttccagata cttacctttt tgaccgttca atgagtacgc gccaaattat
      781 gaaaataatg gtaagtcagt tttggaaaaa agttacaccc gagatgctta aaatggccgg
      841 cgagcttgga tttacccccc atcaatttgt tacctttgcc tctattgtcg gcaaagaatc
      901 aggtgataat gcagaaaaac ctatgatttc agctgtattt cacaataggc tcaaaaagaa
      961 aatgcgcctg caaagcgatc caacggccgt ttatgatttg gataattttg acggccaagt
     1021 cttacgcaaa catttgaaaa gaaattctcc gtataacact tatattatta gaggcttgcc
     1081 gccagggccc attgccaatc cgggattgac ttctttaaga gctgcgcttt atccagcttc
     1141 agttaattat ctttactttg tttccaaaaa agacggttct cacttttttt cagattcctt
     1201 ggatatgcac aatcaagcaa taaatcgtta tcgaatacgt aaaaagtaaa ataatataaa
     1261 cgctaagatt gaacaaaact tatagctgtc ttgtaatcaa aaaaggatgg gaaaacccct
     1321 ttaatcttta aatcagccgt tttccccctg ctatttttcc aattcaaaat tatttctgtt
     1381 tcactttctc tattttccta gtgattttaa gtttcttaat caaaaaaagt taaatttttt
     1441 tcttgacaaa ggcattttac ctctttatag ttggccacgt ggagtaaagt ggcttattgt
     1501 ggaggttagt agtgtctgtt tttcgaggcc aatatcatca taatattgat gaaaagggaa
     1561 gaatcatttt tccgtccaag ttccgggagg tctttgggga gaagtatgac aaccggatgg
     1621 ttatcactaa ctgggatggt tatttgatgg tttttcccta tgatgagtgg cgcatcattg
     1681 aagaaaaagt ttcacaccag tctattttgc gtaaagaagt gcgtgcgttt cagcgcttct
     1741 ttatgtctgg agcagttgac tgtaatttgg atggccaggg gcgaatttta attccaccag
     1801 ctctacgcga ttatgcgcaa ctggaaaaag acataatttt agcgggcatg attaaagtaa
     1861 tcgaaatttg gtcgaaggaa cgttttgaag atgaaatgaa aa
//
LOCUS       DASVIM010000002         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1279784, whole genome shotgun sequence.
ACCESSION   DASVIM010000002 DASVIM010000000
VERSION     DASVIM010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279784"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VF305_00015"
     CDS             <1..592
                     /locus_tag="VF305_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX7415553.1"
                     /translation="GGQCVEISLPHTQYCLAVYYIIAPAEASSNLARYDGVKYGFRAA
                     DARDLLDMYKKTRMQGFGAEVKRRIMIGTYALSAGYYDAYYGKASQVRALIKRDFEEA
                     FKNCDVILTPTTPTPAFKIGEETDNPLQMYLSDIFTISTNLAGIPGISVPCGFTSGGL
                     PIGVQFLASHFEEGKLIQIASLYEKNAKIEKRRPAL"
     gene            592..2022
                     /gene="gatB"
                     /locus_tag="VF305_00020"
     CDS             592..2022
                     /gene="gatB"
                     /locus_tag="VF305_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX7415554.1"
                     /translation="MEFETVIGLEVHAQMLTDTKIFCNCSTKFGAAPNSHTCQICLGM
                     PGVLPVLNKKVVEFAMKMSLATNCEINKSCSFARKNYFYPDLPKGYQISQYAYPLAEH
                     GYVFLDVNGAQKKIGITRIHMEEDAGKLLHDEHNPVSYVDLNRTGVPLIEIVSEPDMR
                     SSEEAADYLKRLHQILVYLEICDGNMEEGSFRCDANVSIRPKGQKEFGTRAELKNMNS
                     FRNVQRALEYEIKRQQYIVENGGTVVQETRLWDDAQSATNSMRSKEEAHDYRYFPDPD
                     LVPILVDKAWVEKIRKELPELPLTKRERFIKDYQIPAYDAGVLTADKALANYYEEVVK
                     LCAQPKVASNWVMGDVLRFLNEEKRDIRQCPITAKSLADMIRLIEEGTISGKMAKDII
                     EEMYKSGQSPQAIIEEKGLVQITDEGELIKAITSIMEANPDQLADYRSGKEKLFGFFV
                     GQAMKATQGKANPQLVNNLLKKMLAG"
     gene            2022..>2276
                     /locus_tag="VF305_00025"
     CDS             2022..>2276
                     /locus_tag="VF305_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX7415555.1"
                     /translation="MIKTISWKNNTVVLIDQNALPSAERYVTCKSYKEVISSIKDLTV
                     RGAPAIGVAAAMGAALGALQLPSFPPEQFRRNFFAICNEIA"
BASE COUNT          650 a          501 c          560 g          565 t
ORIGIN      
        1 tggcgggcaa tgtgtggaaa tttcattgcc gcacactcaa tactgtctgg cggtttatta
       61 tattatcgct ccggcggaag catcttccaa tttggcgcgt tacgacggcg ttaaatatgg
      121 tttccgggcg gcagacgcgc gtgacctcct ggacatgtac aaaaagaccc gcatgcaggg
      181 attcggcgcg gaagtgaaaa gaagaatcat gattggcacc tatgccttgt cggccggtta
      241 ttacgatgcc tattatggaa aagcttctca ggtgcgggcg ttgatcaagc gggacttcga
      301 agaggccttt aaaaattgtg atgtaattct tacacccacc acaccgacac cggcttttaa
      361 aatcggcgag gaaaccgaca atccgttgca aatgtatctt tccgatattt ttacaatatc
      421 gactaactta gcgggaatcc cgggaatttc tgtcccttgc ggatttactt ccggtggctt
      481 gccgataggg gtgcagtttc tggctagtca cttcgaggaa ggaaaattaa ttcaaattgc
      541 ctctctctac gagaaaaacg ccaaaattga aaagaggagg cctgcgttat aatggagttt
      601 gaaacagtca tcgggctgga agtccatgcc caaatgttaa cggatacaaa aatattctgc
      661 aactgttcca ccaaatttgg ggcagcgcct aatagccata cttgccagat ctgtctggga
      721 atgccgggcg tcttaccggt attaaataaa aaagttgtgg aatttgccat gaaaatgtct
      781 ctggcgacga actgtgaaat taacaagtcc tgcagctttg cgcgtaaaaa ttatttttac
      841 ccggacctgc ccaaaggcta tcaaatatcc caatatgcct atccgctggc agagcatggt
      901 tatgttttct tggatgtaaa cggagcacaa aagaaaattg gaatcaccag aattcacatg
      961 gaagaagacg ccggtaaact attgcacgat gaacataatc cggtaagtta cgttgatttg
     1021 aaccgcaccg gtgttcccct gattgaaatt gtcagcgaac cggatatgcg ttcgtccgaa
     1081 gaagcagccg actatctcaa gcgtttacac caaattctgg tttatctgga aatttgtgac
     1141 ggcaatatgg aggaaggctc tttccgctgt gacgccaacg tttccattcg ccccaaaggg
     1201 caaaaggaat tcggcaccag ggcggaattg aaaaatatga attccttccg caatgtgcag
     1261 cgcgcactgg agtatgaaat caagcggcag caatatatcg tagaaaacgg cggcactgtt
     1321 gttcaggaaa cgcgtttgtg ggatgacgcg caaagcgcta cgaattccat gcgcagcaag
     1381 gaagaagcgc atgactaccg ttatttcccc gatcccgatt tagtgccgat tttagttgat
     1441 aaggcctggg tggaaaaaat tcgaaaagag cttcctgaat taccactgac aaaacgggaa
     1501 agattcatta aagattatca gattcccgct tacgacgcgg gcgtactgac ggccgataag
     1561 gcgctggcta attattatga agaagtagtg aagctttgtg cccagcctaa agtagccagc
     1621 aactgggtca tgggtgacgt gttgcgtttt ctcaatgaag aaaagcgcga tatccgtcaa
     1681 tgccccatca cggctaaatc tctggctgac atgattcggc tgattgaaga aggcacgatc
     1741 agcggaaaaa tggccaaaga tattattgaa gaaatgtata agagcggcca gtcgccgcag
     1801 gcgattattg aagaaaaagg tctggtgcaa attacggatg aaggcgagtt gatcaaagca
     1861 atcacctcta tcatggaagc caatcccgat caacttgcgg actatcgcag cggcaaggaa
     1921 aagcttttcg gcttttttgt cggtcaggcg atgaaagcga cccagggtaa ggccaatccg
     1981 caactggtga ataacctgct aaaaaagatg cttgccggat aatgattaaa acaattagtt
     2041 ggaaaaataa cacagtagtt ttaattgatc agaacgcact gccttctgcc gaaagatatg
     2101 ttacctgcaa atcctataaa gaagtaattt catccatcaa agatttaacc gtgcgcggcg
     2161 ctcctgctat cggtgtcgcc gccgcgatgg gcgctgcttt gggagctttg cagcttccat
     2221 cttttccgcc ggaacaattc cgccgaaatt tttttgctat ttgcaatgag attgcc
//
LOCUS       DASVIM010000003         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_682775, whole genome shotgun sequence.
ACCESSION   DASVIM010000003 DASVIM010000000
VERSION     DASVIM010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682775"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..1616
                     /gene="folP"
                     /locus_tag="VF305_00030"
     CDS             423..1616
                     /gene="folP"
                     /locus_tag="VF305_00030"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX7415556.1"
                     /translation="MKIIKIKNLEEAAVIFKSIGVDPYGIDAMASKTININILIENQP
                     CKIANIIKQEMLSVGGDAAVARGSVSCSVPVSDILIMGTLKQILALAKKIEKQPFGLS
                     LIARDMSEILVNISQNEYIWKTSRRKLTLGNKTLIMGILNVTPDSFSDGGLFYSQQKA
                     VEHGLQMAAEGADIIDIGGESTRPGAGFVATGVEAKRVLSVIESLVKQVKIPISIDTK
                     KAQVAKQALASGAEIVNDISALNGDKKMAKTIMGAGAAVILMHMRGNPRNMQKGKLVY
                     DNLMGEITDYLKKSSEKALKAGIEKDCLVIDPGIGFGKIPEDNYKIIKNLSQLKELGM
                     PVMIGTSRKSFIGKVTSGEPGQRLEGTAATVAAAIMNGCHIVRVHDVAAMKKVAAITD
                     AIVHV"
     gene            1627..2007
                     /gene="acpS"
                     /locus_tag="VF305_00035"
     CDS             1627..2007
                     /gene="acpS"
                     /locus_tag="VF305_00035"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX7415557.1"
                     /translation="MVYGIGIDLVENGRIEKIIGKWGSKFLQRVFSDEEIQYCGKHVQ
                     SSIHYGARFAAKESFLKALGIGLGRGVKFKDIEVMHDKNGKPDLVLWGGAKAQTEKKK
                     ITKIHLSLTHTKKYATAVVILEKK"
     gene            2036..2524
                     /gene="tsaE"
                     /locus_tag="VF305_00040"
     CDS             2036..2524
                     /gene="tsaE"
                     /locus_tag="VF305_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX7415558.1"
                     /translation="MNRKNTKIEIISASAQQTWGIARFIGEKLREGDVLALSGELGSG
                     KTCFTGGLARGLGVSEKYQITSPTFTLINEYPARCKLYHFDLYRLSGYSELEDLGYEE
                     YFCGEGVVVIEWAEKITPILPAGTFFINFEYLDEHTRRIEIKGLKSRLKELAIDTKMG
                     VS"
     gene            2526..>2852
                     /locus_tag="VF305_00045"
     CDS             2526..>2852
                     /locus_tag="VF305_00045"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417432.1"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP, in Bacillus, lysine sensitive;
                     regulated by response to starvation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX7415559.1"
                     /translation="MALIVQKFGGTSVADIDKIRNVAQKVINEKKAGNEVIVVLSAMA
                     GETDRLIALAQKAAELPDAREYDSLISTGEQVTVTLLAMVLNSMGYKAKSFLGFQVKI
                     LTDKVHK"
BASE COUNT          929 a          489 c          678 g          756 t
ORIGIN      
        1 cacaagatag ccggggaact tctggagaaa gaagtcttaa acggagcgga aatcgatgcc
       61 ttgattggta atgctttgcc gaatcagcaa caggacgatt cgaaaaaaat atcttaatca
      121 tgagtatgaa cttcttaaca aatttcaacg tgtgacctcg tgtgccctcg cgtcacctcg
      181 tgtgaccttc aggttatcag gtgattaggg tattaggtta tacacttcgt ttttgaatac
      241 agttttccgc gcaatcagcc gttgtcaagc ggcgatggga gcgggaaaat agcaaataag
      301 tatttacctt aagtatggta gctacctgaa aggtagctac caatttgagt ctcaaaaata
      361 aatatctttt gtgactcgtc tggaaaaaat acttaagttt aatttgatat cggtgttgtc
      421 ttgtgaaaat aattaaaata aaaaacttgg aagaagccgc agtgatattc aaaagcatcg
      481 gtgttgatcc ctatggcatt gacgctatgg cttcaaaaac aataaacatc aatattttaa
      541 ttgaaaatca gccgtgtaaa atagccaata ttatcaagca agagatgtta tcagtaggcg
      601 gagatgccgc tgttgccagg ggaagtgttt cttgcagcgt tcctgtgagc gatattttaa
      661 tcatgggtac actaaagcaa atattggcgc tggcaaaaaa aatcgaaaaa cagccctttg
      721 gtttaagtct aatcgctcgc gatatgtccg aaatattagt aaatatcagc caaaacgaat
      781 acatctggaa aacatcccgc cgtaaactta ctttaggcaa taaaactctg attatgggca
      841 tcttaaatgt tacgcccgat tcgttttccg atggcggtct tttttactct cagcaaaaag
      901 ccgttgaaca tggattgcaa atggcggccg aaggcgcgga tattatcgac attggtggag
      961 aatcgacacg tcccggagct ggatttgttg caacgggcgt tgaagcaaag cgagtccttt
     1021 ctgtaattga gagcttggtg aaacaggtaa aaattccaat ttccattgat acaaaaaagg
     1081 cgcaggttgc taagcaggcg ctggccagcg gcgccgaaat agtcaatgat ataagtgcac
     1141 tcaatggcga taaaaaaatg gccaaaacta tcatgggtgc aggcgctgcc gtgattctta
     1201 tgcacatgcg gggaaatccc cgcaacatgc aaaaaggtaa attggtttat gacaacctaa
     1261 tgggagaaat tacggattat ctgaaaaaga gcagcgaaaa agccctgaaa gcaggtatag
     1321 aaaaagattg tctggttata gatcccggaa tcggttttgg gaaaattccg gaagacaatt
     1381 ataaaattat taaaaatctt tcccagttaa aagagttggg aatgccggta atgatcggaa
     1441 cctcccggaa atcttttatc ggcaaagtaa ccagcggcga acctgggcaa agattggaag
     1501 gtacggcggc aactgtcgcc gcggcaataa tgaatggttg ccatattgtc cgggtgcatg
     1561 atgttgcggc catgaaaaaa gttgccgcaa ttacagatgc gattgttcac gtgtagaagg
     1621 aatatgatgg tttatggaat tggcatagat ttagtggaaa acggccgtat cgaaaagatc
     1681 atcggaaaat ggggttctaa atttttgcaa agagtctttt ccgacgaaga aatacaatac
     1741 tgtggaaagc atgtgcaatc gtcaatccat tacggagcga gattcgcggc caaagaatct
     1801 tttctgaaag cattaggtat tggtttaggc cgaggcgtaa aatttaaaga tatcgaagtc
     1861 atgcatgaca aaaatggaaa acctgatctt gtcttatggg gcggggccaa agctcaaacg
     1921 gaaaaaaaga aaatcacaaa gattcacttg agtttgacgc atacgaaaaa atatgccact
     1981 gcagttgtta tattggagaa aaaataaatt aattgtatga ttgccgctat tttaaatgaa
     2041 tcggaaaaac acaaagattg aaataatatc tgcaagcgca cagcaaactt ggggaatagc
     2101 gcgttttatt ggcgaaaagt taagggaagg tgatgtctta gcgttgtcgg gtgaacttgg
     2161 ttccggcaaa acttgtttta ccggcggttt agcccggggc ttaggagtaa gcgaaaaata
     2221 tcagattacg agtccgactt ttactttgat taatgaatat ccggcccgct gtaaacttta
     2281 tcactttgat ttgtacaggc tgagcgggta tagtgaattg gaagatttag gctacgaaga
     2341 atacttttgc ggagagggtg ttgttgttat cgaatgggcg gaaaaaataa ctccaattct
     2401 tcccgcaggc acttttttta taaattttga atatcttgat gaacatacca gaagaatcga
     2461 gataaaaggc cttaaaagca ggctgaaaga attggcaata gatacaaaaa tgggggttag
     2521 ttaaaatggc tttaattgtt caaaaattcg gcggaacatc ggtcgcagat atcgataaaa
     2581 taagaaatgt ggcccaaaaa gtgattaatg aaaaaaaagc ggggaatgaa gtcatagttg
     2641 ttttgtcggc aatggccggt gaaactgacc gcctcattgc cttagcccaa aaggctgcag
     2701 aattgccgga tgcaagagaa tatgactctt taatttctac aggcgaacaa gttaccgtga
     2761 ccttgctggc tatggttttg aactctatgg gttataaggc aaaatcattt ttaggatttc
     2821 aggttaaaat cttgacagat aaggttcata aa
//
LOCUS       DASVIM010000004         2728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1450609, whole genome shotgun sequence.
ACCESSION   DASVIM010000004 DASVIM010000000
VERSION     DASVIM010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450609"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..847)
                     /locus_tag="VF305_00050"
     CDS             complement(155..847)
                     /locus_tag="VF305_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEX7415560.1"
                     /translation="MNRAALHKISYGLYIVTSGQDGKFNGQIANSMFQVTANPATIAI
                     SINKENYTHELIKLSRKFAVSILAEAAPMNFIGLFGFKSGRDVNKLQNTKTKTGVTGV
                     PIVLENAVSYIEAELESELDCGTHTIFVGRIVDADVISNEEPLTYAYYQKVKGGKSSK
                     NAPTYVKEETAAPSTSASARYVCSVCGYVYDPAKGDPDGGIAPGTKFEDIPDSWVCPV
                     CGADKSKFEKEK"
     gene            1220..1810
                     /gene="fabA"
                     /locus_tag="VF305_00055"
     CDS             1220..1810
                     /gene="fabA"
                     /locus_tag="VF305_00055"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HEX7415561.1"
                     /translation="MNYQEFMGRDHFNLEELLGFAYGTLVTDPPEHFDARLPAPPFLM
                     VDRILSIAKDRSQGKIIAEQDIRMDAWYFQCHMPGDPVQPGCLGVDAIWQLLGFYCIW
                     RGALGSGRALGCGEISFNGQIRPFNKCVRYEVDIRRYSRLKESGTSVVIGDGRIYVDN
                     ELIATVQQARTGVFKDIAYKDYPLRSPNSIGGMMVR"
     gene            1990..2355
                     /locus_tag="VF305_00060"
     CDS             1990..2355
                     /locus_tag="VF305_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415562.1"
                     /translation="MSIVKKIALYIARNEFCRKAIAEHANLSVFKKKPSFRIIIGLVL
                     IAVSYVIGLPTAIVLGIIVASQGNAVLAAVAAPLLYGISWLFFLVAMYLTGPEYGRVL
                     SRWAARVILEKILGDEAKT"
     gene            2446..2718
                     /locus_tag="VF305_00065"
     CDS             2446..2718
                     /locus_tag="VF305_00065"
                     /inference="COORDINATES: protein motif:HMM:NF006617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX7415563.1"
                     /translation="MSQLNELIAELKVKIIQTLGLMDVKPEDIKDDSPLVGGDTGIDS
                     IDVLELVMMIEKDYGVKIESKELGAKVFASVRALAAHILANQRQKA"
BASE COUNT          729 a          557 c          603 g          839 t
ORIGIN      
        1 ctaataatca attgattaaa agaaatttat ttataacaag ttccattcat aaggataacg
       61 aggcggcaag gaggaggctc cgcaggttcg ccgcggcgga ttgaggaagc cgacaacgat
      121 gccaacaaag ttagccaaaa aagacagctt gttattattt ttctttctca aacttagact
      181 tatccgcacc gcaaaccggg caaacccagg aatccggtat gtcttcaaac ttcgttccgg
      241 gagcaatacc accatccggg tcgcctttag ccggatcata aacatagccg caaacgctgc
      301 atacataacg cgctgatgct gaagtcgacg gcgctgccgt ttcttctttc acataggtcg
      361 gagcgttttt ggatgatttg ccacccttaa ctttttgata gtatgcataa gtcaagggtt
      421 cttcgttact gataacatcc gcgtcaacaa tgcgtccgac aaatatggta tgcgtaccac
      481 aatccaattc gctttccagc tcagcttcaa tatagctgac ggcgttttcc agaacaatcg
      541 gcacgcctgt cacacctgtc tttgttttcg tgttttgcag tttgtttaca tctcgcccgc
      601 ttttaaaacc gaaaaggccg atgaaattca tcggcgccgc ctctgccaga atcgaaacgg
      661 caaatttgcg gctgagtttg attaattcgt gcgtgtaatt ttctttattg atgctgatgg
      721 cgattgttgc cggattcgct gtgacctgaa acatggagtt ggcaatctgg ccgttgaatt
      781 ttccatcctg tccggacgtc actatataaa gaccgtagct tattttgtgg agtgctgctc
      841 tgttcatttt ttcctctttt ttataaatta ttccattacg atatcttata acgcaaattt
      901 tacctcaacg ctattaaatt ttgtttaata cgcgaaggaa ataagcaccc ttgttttttc
      961 ttatcaagtt aatcctgtta tttcccagcc attgacaaca ttttttctga agatattttt
     1021 cttttgacaa aagagtattt ctcttatagt aaattaatca aataatgaag taaattccaa
     1081 ttatgcaacc tagcaactat ggctggggcc ggggacatta atgcccgcac tggaataaac
     1141 ttcaacctgg atatcattga aactatatca ttaaacactc caccgggatt aaaatttata
     1201 agaaagtgaa aaaacgataa tgaattatca agaatttatg ggacgcgacc attttaatct
     1261 cgaagagctt cttggctttg cttatggaac gctggtaacc gatcctcccg agcattttga
     1321 tgccaggctt ccagcacccc ccttcctcat ggttgatcgt atactctcca ttgcgaagga
     1381 ccgcagtcaa ggtaaaatca tagcggaaca ggatatccgt atggacgcct ggtattttca
     1441 gtgtcatatg ccgggagatc cggtgcagcc cggctgtctt ggcgttgacg ccatctggca
     1501 attgcttggt ttttattgta tctggcgcgg cgcgttgggt tcgggccgag cgctgggttg
     1561 cggagaaatt tcctttaacg gccagattcg ccccttcaat aagtgcgtcc gttatgaagt
     1621 tgacatccgg cgttattcgc gcctgaaaga atcaggaacc agcgtggtaa tcggtgacgg
     1681 ccggatttat gtggataatg aattgattgc tactgtccaa caagcccgga caggagtttt
     1741 taaggatatc gcctacaaag attatccgct tcggtcgcca aattctattg gtggaatgat
     1801 ggtaaggtga tatatattgt gacattatct ttaatggcgg ttgttgattt taatcttcaa
     1861 ccgtcttttt acatctgtaa gctataatgt tctttattta tacttgatat ataaattttc
     1921 cttgaaatta ttttccactt aaagctatca tcacgcactt ttttaaattc gattgtttgc
     1981 ggggatcttt tgagtatcgt aaaaaaaata gcattgtata ttgcccggaa cgagttctgc
     2041 cggaaagcaa ttgctgagca tgcgaatctc agcgtcttta aaaagaaacc ttctttccga
     2101 ataattatcg gtctagtttt gattgccgtc agttatgtta ttggtctgcc gacagcaatt
     2161 gttttgggta taattgttgc atcacagggc aatgccgtat tagccgctgt tgctgctccg
     2221 ctgctttatg gtatttcctg gctgtttttt ttagtggcca tgtatttaac aggacctgaa
     2281 tatggaaggg ttttaagccg ctgggctgcg cgggttattc tggaaaaaat tctcggggat
     2341 gaggcgaaaa cataagcttc tttaaatacg aataattttg gaagcataaa aatgtaaaga
     2401 ctaaaatgcg ctggcgagtg ccagattaaa attttgaggt gtccaatgag tcaacttaat
     2461 gaattgatag ctgaattaaa agtgaagatc attcaaacac tgggattgat ggatgttaag
     2521 cccgaagaca tcaaagatga ttccccgctt gtgggcggtg atacgggaat tgattctatt
     2581 gatgttctgg agctggtgat gatgattgaa aaagactatg gcgtcaaaat cgagagcaaa
     2641 gaactgggcg ctaaggtttt cgcatcggtg cgagcattgg ccgcgcacat tttagcgaat
     2701 cagcgtcaaa aggcttaagc tttcgctt
//
LOCUS       DASVIM010000005         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_341726, whole genome shotgun sequence.
ACCESSION   DASVIM010000005 DASVIM010000000
VERSION     DASVIM010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341726"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..599)
                     /locus_tag="VF305_00070"
     CDS             complement(3..599)
                     /locus_tag="VF305_00070"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HEX7415564.1"
                     /translation="MKIVFASHNKGKVREIKKMLEGMEIDLVSLNDYASAPAIVEDGK
                     SFLENAFKKAKIVAEFTGETVLADDSGLQVEALGGEPGIYSSRYAGENATDEENINKL
                     LAKLKDIPQEKRSAFFCCVLVIYRIDGSHDFFEGKWRGQIIDEPRGNNGFGYDPIFLV
                     PELKMTAAELPAEIKNKISHRGQAFAQLKRSLGGRSNN"
     gene            complement(596..1327)
                     /gene="rph"
                     /locus_tag="VF305_00075"
     CDS             complement(596..1327)
                     /gene="rph"
                     /locus_tag="VF305_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX7415565.1"
                     /translation="MIKRNLDRKFDELRPIRITNNYIKNVAGSVLMEFGNTKVICTAS
                     LDDKPVPFLKNTGKGWLTAEYSMLPMSTQTRTVREAVRGRLGGRTHEIQRLIGRSLRA
                     VTDLTSFGERTIYIDCDVIQADGGTRTASITGGFLALVDLFKRLRQNGTVKEIPISDF
                     VSAISVGIVDNQILLDLEYEEDSQADVDMNFVMTGSGLFVEVQGTAEQVPFNKEQLDK
                     MTDLAANGIQKIIARQKEIVGDLKK"
     gene            complement(1335..2105)
                     /locus_tag="VF305_00080"
     CDS             complement(1335..2105)
                     /locus_tag="VF305_00080"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7415566.1"
                     /translation="MITIAILIVDKQSVSAISKRKIKKGEGMFYKTKKLFTVGIVVSA
                     LFICGTLELSIAEKRHLIVIDPAHGGQDTGVKLTDKVSEKDITLAIALALQKEIVKEN
                     ICEVILTRDSDKTVSLEERKKNIAKIKPDFFLSLHVNAGFGKSAAGFELYYPGFKYPA
                     EEKKMAKGGARKAKNKYLNDSIRLAQIIQKNLDTLFPRKGRGLREADLPIIEDLFVPA
                     LVVEMGFASNPEDKKKLLSPNTQTEIAQALARSIKSYF"
     gene            complement(2109..>2407)
                     /locus_tag="VF305_00085"
     CDS             complement(2109..>2407)
                     /locus_tag="VF305_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7415567.1"
                     /translation="KRLDADRIVDVDWNVKEKHAYPVYIKVVCDDRKGVLTEVSSIIS
                     SFDINISYAQIETIDMIATCNFVIDVNDLHQLNKISSAIRQLKFVKSVERIRQY"
BASE COUNT          678 a          569 c          380 g          780 t
ORIGIN      
        1 ttctaattat ttgatcttcc tccaaggctt cttttgagtt gggcaaaagc ctgtccacga
       61 tgacttattt tgttttttat ttccgcaggc aattcggcgg cagtcatttt taattccggc
      121 accagaaaaa taggatcata gccaaaaccg ttatttccgc ggggctcgtc aataatttgt
      181 ccgcgccatt taccttcaaa aaaatcgtga ctgccgtcta ttcgataaat taccaaaaca
      241 caacagaaaa acgccgatct tttctcctgt ggaatatctt ttaatttggc cagaagttta
      301 ttaatattct cctcgtcagt ggcgttttct ccggcataac gcgaagaata aatacccggt
      361 tcgcctccta aagcctccac ctgcaatccg gaatcatccg ccaaaacagt ttcaccggta
      421 aattcggcga ctatctttgc tttcttgaac gcattttcta aaaaactttt accatcttcg
      481 acaattgcgg gggcactagc ataatcattt aaggaaacta aatcaatttc cattccctcc
      541 agcatttttt taatttctct tacttttcct ttattatggg aagcaaaaac aatcttcatt
      601 tttttagatc acccacaatc tctttttgcc gggcaataat cttttgaatg ccgtttgccg
      661 caagatcggt catcttatct aattgttctt tattaaaagg tacctgctct gccgtgccct
      721 gcacttcaac aaagagcccc gatccggtca tgacaaaatt catatccaca tccgcttgcg
      781 aatcttcttc atattccaaa tcaagaagaa tttgattatc gactattcca acactgattg
      841 ctgatacaaa atcactgatg ggaatttctt ttacagtacc attttgtctc aatcttttaa
      901 ataaatctac aagggctaga aaccctccgg taattgacgc cgtcctggtt ccaccatcag
      961 cttgaatgac atcacaatct atatagatag ttctttcacc aaaagaggtt aaatccgtca
     1021 ccgcccgtaa cgaacggcca atcagtctct gtatttcgtg cgttcggccg cctaaacggc
     1081 ctcggacagc ctcccgcact gttcgcgttt gcgtggacat aggcaacatc gaatattcag
     1141 ctgttagcca accttttccc gtgtttttca agaaaggcac cggtttatca tccaaagatg
     1201 ccgtgcaaat taccttggta ttaccaaatt ccatcaacac ggaaccggca acgtttttta
     1261 tataattatt ggtaatcctg atcggtctta gttcatcgaa ctttctatct aagtttcttt
     1321 taatcacaaa tcacctaaaa atatgactta atactcctgg ctaaagcttg agcaatttct
     1381 gtttgcgtat taggagaaag caatttcttt ttatcttcag gattagaggc aaagcccatt
     1441 tccacaacta aagccggcac aaacaaatcc tcaattatcg gcaagtcagc ttcccttaag
     1501 cccctaccct ttctggggaa taaagtatct aagttttttt gaatgatctg cgccaatctt
     1561 atagaatcat taagatattt attctttgct tttcgggcac cacctttagc catctttttt
     1621 tcttctgccg gatatttaaa ccctggataa taaagctcga atccagcggc actcttacca
     1681 aatcctgcat tgacatggag acttaaaaaa aagtccggtt tgattttcgc aatatttttt
     1741 ttccgctctt ctaggctaac cgttttatcg gaatcacgtg ttaagataac ctcacaaata
     1801 ttctctttga cgatttcttt ttgcaaggct aaagcaatgg ccagtgtaat atctttttcg
     1861 ctaaccttat ccgttaactt aacacctgta tcctgacctc catgcgccgg atcaataaca
     1921 attagatgcc ttttctccgc tatggatagc tccaaagttc cacaaataaa taaagccgac
     1981 actacgatac caaccgtgaa caatttcttt gtcttataaa acataccttc acctttttta
     2041 attttgcgct tgcttatagc agagacactt tgtttgtcaa caatcaatat ggctatagtt
     2101 atcaaatttc agtattggcg tatccgctca acggatttaa caaattttag ctgtctaatt
     2161 gccgatgata ttttattgag ttgatgcaaa tcatttacat caattacaaa attgcacgtg
     2221 gcgatcatat cgattgtttc tatctgggca taactaatgt tgatatcaaa agatgagatt
     2281 atggaactaa cctctgttaa aacgcccttg cggtcatcgc agacaacttt aatatagaca
     2341 ggataagcat gcttctcctt gacgttccaa tccacatcaa caattctgtc tgcatccaat
     2401 cttttga
//
LOCUS       DASVIM010000006         7318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_171195, whole genome shotgun sequence.
ACCESSION   DASVIM010000006 DASVIM010000000
VERSION     DASVIM010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171195"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VF305_00090"
     CDS             complement(<1..585)
                     /locus_tag="VF305_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX7415568.1"
                     /translation="MAKINFGGVMEDVITAEEFSLKKAREILKKEVVAVLGYGVQGPA
                     QAFNMKDNGINVIIGQAKEDKQYWDKAIADGWVPGKTLFSIEEAVQRGTVIQYLVSDA
                     AQKILWPKVKANLKKGDALYFSHGFSIVYKEQTGVIPPDFVDVIMVAPKGSGTSVRRN
                     FLDGSGINSSFAVHQDATGRAMERTIALGIAIGSG"
     gene            complement(638..1123)
                     /gene="ilvN"
                     /locus_tag="VF305_00095"
     CDS             complement(638..1123)
                     /gene="ilvN"
                     /locus_tag="VF305_00095"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX7415569.1"
                     /translation="MEKKEHIISILVHNKPDVLARIAGTLGGKGYNIESLCVNTTTQY
                     EISKIVMTTVGSQETVTRIENQLQRLVDVIAVDDLTNVESIHRELILVRLNLTADKKE
                     QIKKAVDTNKWKVIVSADTYMVLEITGDQSQIDFALVRLEPLGIADITRTGIIALNLE
                     N"
     gene            complement(1151..2854)
                     /gene="ilvB"
                     /locus_tag="VF305_00100"
     CDS             complement(1151..2854)
                     /gene="ilvB"
                     /locus_tag="VF305_00100"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX7415570.1"
                     /translation="MKLKGSQILLKMLEEEKVDIIFGYPGGSVLDIYDQLYKSAIKHI
                     LVRHEQGAVHAADGYARATGKVGVCLVTSGPGATNTVTGIATANMDSIPIVVFTGQVP
                     TGLIGNDAFQESDITGITRPCTKHNFLVRSVEELASTIKEAFHIARSGRPGPVLVDLP
                     KNVMAMETDYDPAKIKIRNYDMFHKPAPKKMAQVLEMLTQAKKPLIMTGGGVILGKAA
                     TELTELARLYQIPVTGTLMGLGSFPGTDPLWLGMLGMHGTFYANMAVSHCDLLIGVGV
                     RFDDRVTGTIETFAANAKIIQIDIDPSSINKNVVVDLPIIGDTKATLTDMINFLAEHN
                     YTLAPAERQEWLKQIVEWKEKVPLTYCQNGKEIKPQHVIETLYKLTKGNAIITTEVGQ
                     NQMWTAQFFPFDHPNTFISSGGLGTMGFGLPAAIGVKCAFPDKQVVDIAGDGSIQMNI
                     QELATAAQYKINVKIVLLNNGYLGMVRQWQELFYEKRYSHTDMTYAPDFVKLAEAFGI
                     VGLRAIKPEEVEPILQKGLSIDKPVLMDFRVSREECVYPMVRPGESISEMELGTREMI
                     N"
     gene            complement(3196..5232)
                     /locus_tag="VF305_00105"
     CDS             complement(3196..5232)
                     /locus_tag="VF305_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX7415571.1"
                     /translation="MTIFRKRSNNENEGEVNKLSVLFTVFLGVIVFILLVTVAGTVTY
                     YILTLDLPDIDALKDYRPSIASHVYDDNNELIDEFFLEDRKIIKIVEVPKIVHYAFVA
                     SEDSRFYQHKGLDIQSIFRAMFKNFEAGTIVQGGSTITQQVAKMMYLSPEKKYTRKVK
                     EAILAYKIDKYLNKDEILNLYLNQIYLGHGTYGIESAASGYFGKSAKDLNLPEAALLA
                     GLPKAPSNYSPFLHYEKAKQRQSYVLTRMVEDGYISQAEMDKAAAAPLQLRSTRPKEK
                     IAAYFVENVRRYVQEKYGADILYKEGLSIYTTLNMSAQKEARDAVEKGLTELEERQKY
                     ERGLVQGALFCMDVKTGAIRAMIGGRDFDKSEFNRATQSRRQPGSAFKPLIYTAAFDK
                     GLNPSTRFVDSPVVIEDPTAEDGLWKPKNFDEKFLGPITMRTALVQSRNIVTIKILQE
                     IGADYAASYATNMGISSPLVRTLSLALGVSGVTLQELVQAYGVLANGGKKVTPYFIKK
                     IVDRTGNIFEETKPQTEQVIDPRIAFMTSYVMQDVVESGTGRRVRSIGRPVAGKTGTT
                     NDTRDAWFIGFTPSLITGVWVGFDQEVSLGKQEVGGRAAAPIWLYFMEKVLQNTPVEA
                     FPTPEGIVFVKVDPKTGQPTGGSEKGTIYECFLSNAQPSERTNGSTEEKEDLFR"
     gene            complement(5247..5999)
                     /gene="tatC"
                     /locus_tag="VF305_00110"
     CDS             complement(5247..5999)
                     /gene="tatC"
                     /locus_tag="VF305_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX7415572.1"
                     /translation="MENNEEHKMSLTEHLIELRKRLIRSVIILGIGFGVCYYYKDWVF
                     DIITKPLTRVLPKNGYLIYTGLTEAFFVYMKLAFFASLIITSPFILYQIWKFISPGLL
                     LKEKKYVVPFVLSSSLLFISGVLFGYFIALPPAFEFFVSFNNKYLQAMISFKDYLSLF
                     VTFLLGFGLSFELPIFIFFLTKLGIVNAKMLSKQRRYAILVIFIVAAILTPSPDALSQ
                     ILMAIPLMFLYEVSIFVARFAEKKKITPENNE"
     gene            complement(6059..6331)
                     /gene="tatB"
                     /locus_tag="VF305_00115"
     CDS             complement(6059..6331)
                     /gene="tatB"
                     /locus_tag="VF305_00115"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX7415573.1"
                     /translation="MFGIGMQELILIAVIALIVVGPKKLPDLAKTLGKGFSEFKKATE
                     GITDELKQTMQNDEKPKDDGLKDSLLLKKGDVEETKPDSSNNASNK"
     gene            6740..>7318
                     /locus_tag="VF305_00120"
     CDS             6740..>7318
                     /locus_tag="VF305_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX7415574.1"
                     /translation="MSRKTGIVKDKRYLHHSAGIAHPESPERLAAIYEMLENPSMAGK
                     FTQIKPREATHKEIETIHSPSYIEFIASTAGERSVFLDPDTATSPESYEIAKLAIGGV
                     SNAIDSVIEGKVDNAFALVRPPGHHAEKDTAAGFCIFNNIAIGAMHAVLKHRLKRILI
                     VDWDLHHGNGTQHSFYSDPRILYFSTHQYPYYP"
BASE COUNT         2002 a         1648 c         1505 g         2163 t
ORIGIN      
        1 cccggagccg atggcaatac ccagagcaat tgtccgctcc atggcgcggc cggtggcatc
       61 ctgatgcacg gcaaagctgg agtttatgcc gctgccgtcc agaaaatttc tgcggacact
      121 ggttcccgat cctttaggcg caaccatgat aacgtctaca aaatcaggcg gaataacgcc
      181 tgtttgctcc ttatagacaa tggagaaacc gtgtgagaag taaagggcat cacccttttt
      241 caggttggcc ttaactttgg gccacagaat cttttgggca gcatcggaaa caagatactg
      301 aataaccgtg ccgcgctgga ctgcttcttc aatagaaaaa agcgttttac cgggcaccca
      361 gccatcggcg atggccttgt cccagtattg cttatcttcc ttggcctgac cgataattac
      421 attaatgccg ttatctttca tattaaaagc ttgtgccggg ccctgaacgc cataccctag
      481 cactgccact acctcttttt ttaaaatttc ccgagctttt tttagagaaa actcttccgc
      541 tgtgatgaca tcttccatga caccgccaaa attaattttt gccataatac ccccccataa
      601 ttaatgttaa taaaatttat tatttttaac caagttatta attttccaag ttcaatgcaa
      661 taataccggt tcttgttata tcagcgatac ccagaggctc gagacggaca agagcaaaat
      721 cgatttgtga ctgatcgccg gttatttcta agaccatata tgtatcggcg gacactatta
      781 ctttccattt atttgtgtca acggctttct ttatttgctc ttttttgtcc gctgttaaat
      841 tcaagcggac aagaatcagt tcacggtgaa ttgattcgac attggttaag tcatccacgg
      901 caatgacatc gacgagcctt tgcaattgat tttcaatacg agtgacagtt tcctgagagc
      961 ccacggtggt cataactatt tttgatattt cgtattgcgt ggtcgtgttc acgcagagac
     1021 tttcaatgtt gtatcccttc ccgcccagcg ttccggcgat tcttgccaat acatccggtt
     1081 tgttgtgaac aagtatcgaa attatgtgct cctttttttc catgctgatt aacctttctc
     1141 cctatattta ttaattgatc atttctctcg tacccaactc catttcgcta atggattcac
     1201 ccggccgcac cataggatac acgcattctt cacgtgatac ccggaaatcc atcaagaccg
     1261 gcttgtcgat ggaaagccct ttttgcaaaa tcggctctac ttcttccggc tttatcgcgc
     1321 gcaagccaac aatcccaaaa gcttccgcta atttcacaaa gtcgggggcg taagtcatat
     1381 cagtatgtga atagcgtttt tcgtaaaata gttcctgcca ctggcgaacc atgcccaggt
     1441 agccattgtt caaaaggact atttttacat tgattttgta ctgggcggct gtagccagct
     1501 cctggatatt catctggatg ctgccgtcgc cggcgatatc tacgacttgc ttatcgggaa
     1561 aggcacactt gacaccgatg gccgcgggca ggccaaaacc cattgtcccc aatccgcccg
     1621 aagaaataaa agtgttggga tgatcaaaag ggaaaaactg cgcggtccac atctgatttt
     1681 ggccgacttc cgttgtaata attgcgttac ctttagttaa cttgtatagt gtttcaatta
     1741 catgttgcgg tttaatttcc ttgccgtttt gacaatatgt cagcggcact ttttccttcc
     1801 actcaacaat ctgtttaagc cattcctgcc gttctgcagg tgcaagtgta taattgtgct
     1861 ccgctaaaaa attgatcata tctgtgagag ttgctttggt atcgccgatt atcggcaaat
     1921 caacaacaac atttttatta atggaggaag gatcaatatc gatttgaata attttggcgt
     1981 tggccgcaaa ggtttcaatt gtaccggtaa cgcggtcgtc gaaccttact cctacgccaa
     2041 taagcaagtc acagtggctg acggccatat tcgcatagaa ggtgccatgc atacccaaca
     2101 tgcccagcca aagcggatcg gtgcccggaa aagaacccag tcccatcaag gtgccggtga
     2161 cgggaatttg atagagtctt gccagttcgg tcagctctgt cgcagctttg cccagaatga
     2221 caccgccgcc ggtcataatt aaaggctttt tagcctgagt cagcatttct aaaacctgag
     2281 ccattttctt gggggccggt ttgtgaaaca tgtcgtaatt tctaatcttg attttagcgg
     2341 gatcgtagtc ggtttccatt gccatcacgt tttttggcaa gtcaactaaa accggacccg
     2401 gccgccctga tcgagcaata tgaaaagctt ctttaatggt tgaagccagc tcttccacgc
     2461 tgcgaaccag aaagttatgt ttggtgcagg gacgtgttat acccgtgatg tccgattcct
     2521 gaaaagcgtc attgccgatt agccccgtgg gaacttggcc cgtaaaaacg acaataggta
     2581 tagaatccat attggccgtg gcaatgccgg taaccgtatt ggtggcgccg ggaccggatg
     2641 taaccagaca aactcctact tttccggtag cgcgggcata accgtcggcg gcatgcactg
     2701 ctccttgttc atgacgaact aaaatgtgtt ttatcgcgct tttgtaaagc tgatcataaa
     2761 tatccaggac tgacccgccc gggtatccga aaatgatatc cactttttct tcttccagca
     2821 ttttcagcag gatttgtgaa cctttcaatt tcatttaacg acccccaaaa atttatcatt
     2881 taatttatac gtcgggtaat aacccgtaaa actaaaaaac cgctagtttc tttgaggaaa
     2941 aagcggcttt gtttctaaaa caaaaaaagc cgcgggcatc aggggcccgc ggcttgtatc
     3001 tcttattttt atttcttaaa gatacacagc ggcagacccc gtaagtacta ctactacgag
     3061 taccaatact ctggtcagta tctggatgac gccgctttct aaatttttaa gaaacataat
     3121 tcattcctgt taaattcgct ccctgtatag ctgggaaaaa aatgtttgtc aagcccttta
     3181 taaatttaga gttttttatc tgaataaatc ttctttttct tcggttgagc cattagttct
     3241 ttcgctgggc tgtgcgttac tcaagaagca ttcataaatt gttccttttt ccgatccgcc
     3301 ggtcggctgt cctgttttgg gatcaacctt gacaaacacg ataccttccg gcgtgggaaa
     3361 agcttccacg ggagtgtttt gcagaacctt ttccataaaa taaagccaaa taggggccgc
     3421 tgctcttccg cctacttctt gttttcccaa agacacttct tgatcaaagc ctacccaaac
     3481 tccggttatt agggaaggag taaaaccaat aaaccaagcg tcacgagtat cattagtcgt
     3541 ccccgtcttt ccggccaccg gtcttcctat acttctgacc cgcctgccgg tgccactttc
     3601 cacaacatcc tgcataacgt aagaagtcat gaaggcgata cgaggatcaa taacttgttc
     3661 cgtctgaggt ttagtttctt caaaaatatt accagtgcga tcaacaattt ttttaatgaa
     3721 ataaggcgtc acttttttgc caccattcgc caaaacacca taagcttgaa cgagttcctg
     3781 tagcgttacg cccgatacgc ctaacgctaa tgataaagtc cgcactaaag gagaagaaat
     3841 acccatgttc gtggcgtatg aggctgcgta atctgcaccg atttcttgca aaattttaat
     3901 tgtcacaata ttgcgtgatt gaacaagcgc cgttctcatt gtaatcggcc ctaagaattt
     3961 ttcgtcgaag tttttcggtt tccagagacc gtcttctgca gttggatctt caatgacaac
     4021 gggagaatca acaaatcgtg tcgatggatt caagcccttg tcgaaggctg ccgtatagat
     4081 cagcggctta aaagcagaac caggctgcct gcgggactgg gtcgcgcggt tgaattcact
     4141 tttatcaaaa tcacggcccc cgatcatcgc gcgaatagcg cccgttttca catccataca
     4201 gaagagcgca ccctgcacaa ggccgcgttc atatttttgt ctttcttcca attccgtcaa
     4261 gcctttttct acagcgtcac gagcctcttt ttgcgcggac atattcaaag tcgtataaat
     4321 ggaaaggcct tctttataga gaatatcggc accatatttt tcttgcacgt agcggcgcac
     4381 gttttccaca aaataagccg caattttttc tttgggtcta gttgatctta actgcaacgg
     4441 agcggcagcg gctttatcca tttctgcctg tgatatatag ccgtcttcca ccatgcgtgt
     4501 aagaacgtaa gattgtcttt gtttggcttt ttcataatga agaaatgggg aatagttgct
     4561 gggagcttta ggtaaaccag ctagcagcgc cgcctcaggc aggttcagat ccttggcgct
     4621 tttgccgaag taacctgatg ctgccgattc tattccgtaa gtgccgtgtc ccagataaat
     4681 ttggtttaaa tataaattta gaatttcatc tttattcagg tacttatcaa ttttgtaagc
     4741 aagaattgct tccttaactt tgcgtgtgta ttttttttcc ggcgaaaggt acatcatctt
     4801 ggctacttgc tgcgtaatgg tgctgccgcc ctgaactatt gttcctgctt caaaattttt
     4861 aaacattgcg cgaaaaatgc tttgtatgtc caaaccctta tgctgataga agcgggagtc
     4921 ttccgaggca acaaaagcat agtggactat cttcgggact tcaacgatct tgatgatttt
     4981 tctgtcttcc aggaaaaatt catcaatgag ctcgttgttg tcatcgtaaa catgagaagc
     5041 gatgctgggg cggtaatctt taagtgcgtc aatatccggc aaatccaagg tcaagatata
     5101 gtaggtaaca gttccagcga cggtcacaag taagataaaa acaattacgc ccagaaaaac
     5161 ggtaaaaagc acggaaagtt tgttaacttc cccttcattt tcattatttg atcttttacg
     5221 gaatatcgtc ataattttac caactattat tcattatttt cgggagttat ttttttcttt
     5281 tcggcaaatc tggccacaaa tatactcact tcatacaaaa acatcaaggg gattgccatt
     5341 aatatttggc ttaaagcatc gggtgaaggt gttaaaattg cggcaacgat aaaaataact
     5401 aaaatagcat accttcgttg tttggaaagc attttagcat ttactatccc tagtttggtc
     5461 aagaaaaaaa taaatatcgg caattcaaaa gataacccaa accccagtaa aaatgttacg
     5521 aaaagcgaca gataatcttt aaaagagatc attgcctgta aatatttatt attaaaactg
     5581 acaaaaaatt caaaggcggg aggcagggcg ataaaatagc cgaataaaac accacttata
     5641 aaaagcaaag aggaagaaag cacaaaagga accacatatt ttttttcctt aagcaataat
     5701 cccggcgaaa taaatttcca tatttgataa aggataaaag ggctggtaat tattagggaa
     5761 gcaaaaaaag ctaatttcat gtaaacgaaa aacgcttccg tgagtccggt ataaataaga
     5821 taaccgtttt taggaaggac tctggtgaga ggtttggtaa ttatatcgaa aacccaatcc
     5881 ttataatagt aacaaacgcc aaagccaata cctaaaataa tcaccgagcg gataagcctt
     5941 ttgcgcaact ctatcaagtg ttcggtcaga gacattttgt gttcttcgtt attttccata
     6001 atttgccgtt ttgcgtattc ttaattttac aagttaggaa aaattatttt tgaatttatt
     6061 atttgttgga ggcgttattg gacgaatccg gtttggtttc ttcaacatca ccctttttca
     6121 gtaaaagtga atctttcagg ccatcgtctt ttggtttttc gtcgttttgc atggtttgtt
     6181 taagctcatc ggttattccc tcagttgctt ttttgaattc gctgaagcct ttgcccaaag
     6241 ttttggccaa gtccggcagc tttttaggac caacgacgat cagagctatt acagcaatta
     6301 aaataagctc ctgcatacca atgccaaaca ttgattatca actcctatca aaaatgatta
     6361 ttaacctata tattgaatcc tgtttttgtc aatatttttt gcaagcgcta ttcatgaagg
     6421 gttgatcgaa tttgttatac caattctaaa tcaaagcgct atctttgttg gcatcgtcgt
     6481 cggcttcctc gacgtgttac ttctgggagt gcatccccca agggggcaca ataaaaacag
     6541 atcattccct taccggtgag ctacgccatc atggcgtggc gctcatatgc ctcgtcgcct
     6601 cctccttgcc gcctcgatat catctttgat ttagaaatgg tattgtaaga taaagagtag
     6661 gggaaatatt ttgctcttta aagccattta tgataaacga gccacagttt tgacgtggga
     6721 gtgatgaaag aaggtgcgct tgtccaggaa aacaggaatt gtaaaagata agaggtactt
     6781 acatcattcg gctggaattg cccatccgga aagtccggaa aggttagccg caatttatga
     6841 aatgctggaa aatccttcaa tggctgggaa attcacacaa atcaaaccgc gtgaagccac
     6901 ccacaaagag atcgaaacta tccattctcc ttcctacatt gaatttattg ccagtactgc
     6961 gggagagcgt agtgtatttc tcgatcctga tacggcaacc agtccggagt cctacgaaat
     7021 agcaaagtta gcaatcggag gtgttagtaa tgctattgat agcgttatcg aaggcaaagt
     7081 agacaatgct tttgctctgg tacgacctcc ggggcaccat gcggaaaaag ataccgccgc
     7141 tggtttttgt atattcaata atattgccat cggggcaatg cacgccgtct taaagcatcg
     7201 cttgaaaaga atactgattg tggattggga tttgcaccat ggtaatggca cccaacacag
     7261 cttttatagt gatccgcgga ttttatattt ttccacccac cagtacccat attacccg
//
LOCUS       DASVIM010000007         3470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_768383, whole genome shotgun sequence.
ACCESSION   DASVIM010000007 DASVIM010000000
VERSION     DASVIM010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768383"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /gene="dnaG"
                     /locus_tag="VF305_00125"
     CDS             complement(<1..1328)
                     /gene="dnaG"
                     /locus_tag="VF305_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX7415575.1"
                     /translation="MHFDDSKIEEIKSRVDIVELTSEYLTLKKTGRNFIGLCPFHQEK
                     TPSFTVNREKQIFYCFGCGEGGNAITFLMKIADKTFPEAIKDLAEKTGVVLPPRFSGK
                     ESRQKDSLKEDITNLNLRAAQQFTRNLFSPAGKVAREYLQNRGISDETIKQFRLGYVP
                     DTWRSLTDHIEGSGLSLKLAEQAGLVIAGKERGFYDRFRGRLIFPIENITGEIVAFGG
                     RILEKGEPKYLNSPESPIYIKGKNLYGLNKAKEEIRKKGFALIVEGYFDLISLWNAGI
                     GNVVATLGTALTREHLELLRRYTPKVVALFDPDEAGKKALDRSLELFLSMRMHARALI
                     LPEGCDPDDYVKKYGKDKLDELISHAQPLSDYYIENVLGGGKTFEDKREMIKTAMEFI
                     SKISDKKEKDLFIKRIAEKTGVNEELLIKETYKNEMQIEPKSGKEKLQFNV"
     gene            complement(1400..1849)
                     /locus_tag="VF305_00130"
     CDS             complement(1400..1849)
                     /locus_tag="VF305_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX7415576.1"
                     /translation="MMDINAQLNKEMVIAAKAKNKIRLSAIRLLKTALHNKEIDLMRP
                     LNEAEILQLLSAIVKQRKDSIEQFAKGGRTDLVEKEEAELKVVQEFMPAQMSDDEVEA
                     IIKKAIAEVSAVSVKDMGKVMKILMPQLTGRADGKVVGEKVKALLSP"
     gene            complement(1866..2210)
                     /locus_tag="VF305_00135"
     CDS             complement(1866..2210)
                     /locus_tag="VF305_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX7415577.1"
                     /translation="MPDCIFCKIVRGEIPCTKVYEDDKVLAFDDIHPMAPVHVIIIPK
                     QHISTLMDIDAKNIDIVNNLIAAAQKVSQIKGITAKGFRTVINCNAEGGQVVFHLHMH
                     VLGGKKLQDELG"
     gene            complement(2233..2967)
                     /gene="hisA"
                     /locus_tag="VF305_00140"
     CDS             complement(2233..2967)
                     /gene="hisA"
                     /locus_tag="VF305_00140"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX7415578.1"
                     /translation="MVIIPAIDIKDGKCVRLAQGDFDRVTVYADNPVDMAIAWAQKGA
                     ELIHIVDLDGSVVGLPRNTRIILDIVKKVNIPIQVGGGIRDMETIEFYLNNGISSVIL
                     GTAALQDEKIIYDAGKTFPLKIILGIDALEGKVAIRGWTQKTNRNVVDIARCYENCGI
                     KAIVYTDIKRDGMETGVNIEATKTLAMSVNIPVIASGGVATIADVEKLLAIKDCEIYG
                     VIIGRALYTGAISLEEAIKKSKKLLN"
     gene            complement(3138..>3470)
                     /locus_tag="VF305_00145"
     CDS             complement(3138..>3470)
                     /locus_tag="VF305_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415579.1"
                     /translation="KPLLKVLKIVGNELDADILAVGYIYRYTERVGYNYSSEHPASVA
                     FEIHLINAKDGSVVWRGAFDKTQKSLMEDVFQASSFFKGGGKWLTARQLSKQGMDEIF
                     ETFPGFER"
BASE COUNT          951 a          846 c          609 g         1064 t
ORIGIN      
        1 acattaaact gcagtttttc ttttccactc ttcggctcta tttgcatttc atttttataa
       61 gtttctttta taagcaactc ttcattgacg cctgttttct ccgctatccg tttaataaaa
      121 aggtcttttt cttttttatc gctgatctta ctaataaatt ccatggcggt ttttatcatt
      181 tcccgtttat cttcaaaagt tttaccaccg cctaaaacat tttcgatata atagtcactg
      241 agcggttgag cgtgagaaat cagttcgtcc agtttatctt taccgtattt ttttacataa
      301 tcgtcgggat cgcagccttc cggcaagatc aacgcccgcg catgcattct catgcttaaa
      361 aatagttcca ggctgcggtc gagagctttt ttccctgcct catccggatc aaaaagggca
      421 acaactttcg gagtataccg gcgtaaaagc tcaaggtgct ccctggttag cgcagtacct
      481 aaagtggcga caacgttgcc aatacctgca ttccatagag aaatcaaatc aaaataacct
      541 tcaacaatga gagcaaaacc ttttttgcga atttcctctt tcgccttatt caaaccatat
      601 aaatttttcc ccttaatata aatgggagat tcgggcgaat tcaaatattt aggttcgcct
      661 ttctccaaaa ttcttccgcc aaaagccact atttcaccag tgatattttc aatcggaaaa
      721 ataagccgtc cacgaaaccg gtcgtagaac cccctttctt taccggcaat aaccaagccc
      781 gcttgctcgg ccaatttcaa ggataagccg ctgccttcga tatgatctgt caaagaacgc
      841 caagtatccg gcacatagcc cagccggaat tgtttaatag tttcgtcaga gatgccccta
      901 ttttgcaggt attcacgagc aaccttgccg gcgggagaaa agagattgcg cgtaaattgc
      961 tgcgccgctc ttaagtttaa attagttatg tcctccttca gcgaatcctt ctgccggctt
     1021 tctttgccgg aaaacctagg cggcaaaaca actcccgttt tttccgccaa atctttaatc
     1081 gcttccggaa aagttttgtc ggcaattttc atcaaaaacg taatggcatt gccgccttcg
     1141 ccgcagccaa aacaataaaa tatctgtttt tcacgattga ctgtaaatga cggagttttt
     1201 tcctgatgaa atgggcacag cccaataaaa ttccggcctg ttttttttaa tgtaaggtat
     1261 tcggaagtaa gctcgacaat atctactctg cttttaattt cttcaatctt gctgtcatca
     1321 aaatgcactg actatcctga ctaaaatata tataaatttt taatcccgac gccttattac
     1381 cggaagaaaa ataaactttt catggggaca aaagcgcttt aactttttca cccaccactt
     1441 taccatcagc cctgccggtt agttgcggca tcaagatttt cattactttc cccatgtctt
     1501 tgacagaaac agcgctaact tccgcaatcg cctttttgat tatagcctca acctcgtcgt
     1561 ccgacatttg cgcgggcatg aattcctgca caacttttag ttccgcctcc tctttttcca
     1621 caagatcagt ccttccacct ttggcgaact gttcaatgga atccttcctt tgtttaacta
     1681 tcgccgagag aagctgcaga atttcagctt cgtttaacgg ccgcatcaaa tcaatttctt
     1741 tattatgcaa tgctgttttc agcaagcgaa tagcggaaag cctgatttta tttttggctt
     1801 tggcggcaat aaccatttct ttgtttaact gggcatttat atccatcatc ctcaccttct
     1861 tttatctagc ctaattcgtc ttgaagtttt ttgcctccaa gaacgtgcat gtgtaaatga
     1921 aacaccacct gccccccttc agcattacag ttaatgaccg tgcggaaacc tttggcggtg
     1981 atgcctttga tctgggacac tttttgcgca gcggcaatca aattattgac aatatcgatg
     2041 tttttagcat caatatccat cagcgtggaa atgtgttgtt taggaatgat gataacatga
     2101 accggagcca tcggatgaat atcatcaaaa gccaacactt tatcgtcttc ataaaccttg
     2161 gtgcagggaa tctcgccccg aactatttta caaaaaatac aatccggcat aattgccacc
     2221 tccccaactt tttcagttaa gaagcttttt gcttttttta atcgcttctt ccagagaaat
     2281 agcacccgtg taaagagcac ggccaataat gactccgtaa atttcacaat cttttattgc
     2341 cagaagtttt tccacatcgg cgatagtagc tacaccaccg gaagcaatta ccggaatgtt
     2401 tacagacatc gccaatgtct ttgttgcttc aatattgacg cctgtttcca tgccgtcacg
     2461 cttgatatcg gtataaacaa tagcttttat cccgcagttt tcataacacc gggcaatatc
     2521 aacaacgttt cgattagttt tttgtgtcca gccccggatg gccacttttc cttccaaggc
     2581 atcaatgcct aaaataattt tcagcggaaa ggttttgccg gcatcataga taattttttc
     2641 atcttgcagt gccgctgtgc cgaggataac gctggagata ccattattta agtaaaactc
     2701 aatggtctcc atatccctta ttccgccgcc aacctgaatt ggaatattaa cctttttaac
     2761 aatatccaga ataatccttg tgttacgggg caaaccgact acagaaccgt caagatcaac
     2821 tatatggata agctcggcgc ctttttgcgc ccaggctatg gccatatcca caggattatc
     2881 cgcatatact gtaacgcggt cgaaatctcc ctgagccagc cgcacacatt ttccatcttt
     2941 aatatcaatt gctggaataa tgaccaagca aatactcccc agcgggaaaa agttcggaat
     3001 catttaacta aaaattgaaa cttccataat agcgaggtgc gaacaatttt caatgcttaa
     3061 ggtaaatttt attttccgcc cgttaaccct cgccaacaac cgggattatg tgggctgctg
     3121 catgccgaaa agtatattta acgttcaaag ccgggaaatg tctcaaaaat ttcatccatg
     3181 ccttgtttcg aaagttggcg cgcggtaagc catttgccac cacccttaaa aaacgaagat
     3241 gcctgaaaga catcctccat caatgatttt tgcgttttgt caaaagctcc tctccacact
     3301 acactaccgt cttttgcgtt aatcaaatga atctcaaaag ccacagatgc aggatgttcc
     3361 gatgagtagt tgtaacccac tctttcggta tagcggtaaa tatatcccac ggctaaaata
     3421 tccgcgtcca attcattgcc aactattttt aagactttca gtaaaggttt
//
LOCUS       DASVIM010000008         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_939265, whole genome shotgun sequence.
ACCESSION   DASVIM010000008 DASVIM010000000
VERSION     DASVIM010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939265"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VF305_00150"
     CDS             <1..533
                     /locus_tag="VF305_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7415580.1"
                     /translation="MTEISRVEFLLQALKSFSIFENPNIQNIILEEFLINFISLVSND
                     LSQSGIKISKLIVPPVEKINADQRLLHQVMLNFITNASDALNNCENPNIFIRAEKKDG
                     FVAITVEDNGCGMSEEAQTNLFKPFYTTKTQGTGLGLTITLKMVTLMKGHMEIKSLKD
                     VGTKATVFIPEGKKTE"
     gene            556..1902
                     /locus_tag="VF305_00155"
     CDS             556..1902
                     /locus_tag="VF305_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7415581.1"
                     /translation="MDDNDIFCESVRDILSDGSTEVITANTGKEGLKICTETKIDVVI
                     LDQKLPDTEGVSLCPSILQQNDQTKIIFVTAYPSFSNAVEAIKVGAHDYLSKPFEMAE
                     LELTVRQAFRTQELEKTEQIQNYHDNKESEEIDLISGQGVFDEIRKMIDLAAASDAPV
                     LITGETGTGKNVVARAIHYKSNLPKKLFLTTNCAAFPESLIEAELFGSEKGAFTGAIS
                     LRRGIFELADGGTLVLDEIGSMPIHLQSKLLGVLEEKRIRRVGGELFKPINVRIIAIS
                     NSDLEEAIKNKLFRDDLYYRLSVIRIHIPPLRERKQDIPKLCEYFINKMARDTNIRLA
                     DSELNKLIEYEWQGNVRELKNVIERSLIIQRGHSLRPSQLLRINHNFLSSAVDNHRIK
                     ESGVITLKIMEKQCIGQTLEKYRGNYSQTAKALGISLSTLKRKIKVYNLTNNILIA"
BASE COUNT          659 a          360 c          381 g          538 t
ORIGIN      
        1 ctatgactga aatttctcgc gtcgaatttc ttttgcaggc gctaaaaagt tttagcatat
       61 ttgaaaatcc taatattcaa aacataatat tagaagagtt cttaatcaac tttatctcgc
      121 tggtctccaa tgatttgagt caatcaggaa ttaaaatcag caaactaatt gtacccccgg
      181 tggaaaaaat aaatgctgac cagcgtcttt tacaccaagt catgcttaat tttattacca
      241 acgcttcgga tgctttaaat aactgcgaaa atccgaatat tttcattcga gctgaaaaaa
      301 aagacggctt tgtggctatt acggtcgaag acaatggttg cggtatgtcc gaagaagcgc
      361 aaactaatct cttcaaacca ttttatacaa cgaagacgca aggcaccggt ttgggtttga
      421 cgattacttt aaaaatggtg actctcatga agggccatat ggaaataaaa agtctgaaag
      481 atgtaggaac aaaagctacc gtgttcattc ctgagggtaa gaaaactgaa taaacctaaa
      541 agaaagattc tcatcattga cgataacgat atcttttgtg aatcagtgcg ggatatcttg
      601 agtgatggat caacggaagt tatcaccgct aatacaggca aagaaggatt gaaaatctgt
      661 accgaaacaa aaattgatgt agtcattttg gatcaaaagc tgcccgatac ggaaggggtt
      721 tccctctgcc cttctattct gcaacaaaac gaccagacaa aaattatttt cgtcacggcc
      781 tatcccagct tcagcaatgc cgtagaagca attaaagttg gcgctcatga ttatctttcc
      841 aaaccttttg aaatggctga gttggaactt acagtgagac aggcttttcg cacacaagaa
      901 ttagaaaaaa ccgaacaaat acaaaactac catgataata aagaaagcga agaaatagat
      961 cttatctccg gtcaaggagt ttttgatgaa atacgtaaaa tgatcgacct ggcagcagcc
     1021 agcgacgcac cggttcttat tacgggagaa acgggtacag gcaaaaatgt tgtggctcgc
     1081 gctattcatt ataaaagcaa tctacctaaa aaattatttc tcacaacaaa ttgcgccgct
     1141 tttccggaga gtcttatcga ggcagaatta ttcggttcgg aaaaaggcgc ttttaccggt
     1201 gcaatatctt tacgccgagg aatttttgaa ttagccgatg gaggaactct tgttttagat
     1261 gaaatcggtt ctatgcccat tcatttacaa tcgaaattat tgggtgttct ggaagaaaag
     1321 cgaattcgtc gcgtcggtgg agaattattt aaacctatca atgtccggat tattgccatt
     1381 tctaatagtg atctcgaaga ggcaataaaa aataagcttt ttcgtgatga cctttactac
     1441 cggctcagcg ttatacgcat tcacattcct ccgctgagag aaagaaagca agatattcct
     1501 aaattatgtg aatattttat taataaaatg gctagagata caaatatccg gctggctgat
     1561 tccgaactca ataaattgat agaatatgaa tggcagggaa atgttcgcga gttaaaaaac
     1621 gtgatagaaa gatctttgat tattcaacgc ggacattctc taaggccgtc acaacttctc
     1681 agaattaatc ataatttttt atcttccgcc gtcgacaatc acagaattaa agaaagtgga
     1741 gttataacgc tgaagataat ggaaaaacaa tgtataggac aaacgctgga aaaatatagg
     1801 ggaaattact ctcaaacggc aaaggcactg ggtatttctt tatccacttt aaagagaaaa
     1861 ataaaagttt acaatctgac caataacatc ttaattgctt agcactaggt cataatgagc
     1921 cagtcatttt ggccttcc
//
LOCUS       DASVIM010000009         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_683558, whole genome shotgun sequence.
ACCESSION   DASVIM010000009 DASVIM010000000
VERSION     DASVIM010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683558"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VF305_00160"
     CDS             complement(<1..340)
                     /locus_tag="VF305_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7415582.1"
                     /translation="MDKIADFLFEVGMLSKTPRSGYQFLGSGKESVAEHVLRTVFVGY
                     ALCKLDDSLDELRVLKMCVLHDLPEARTGDMNYVNKKYVDVDEEKAVQELTEELFFGK
                     EIKAAIEEFNR"
     gene            complement(342..560)
                     /locus_tag="VF305_00165"
     CDS             complement(342..560)
                     /locus_tag="VF305_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415583.1"
                     /translation="MASMDSSNAFIKEYQERFEKKLRENEIALLEHWKAQLDKIVSMR
                     PDSIASLQVSITKTAEMMANRIKVLKKE"
     gene            710..1579
                     /locus_tag="VF305_00170"
     CDS             710..1579
                     /locus_tag="VF305_00170"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX7415584.1"
                     /translation="MPDKPNPEDIAGGQALIEGVMMRHGNKIAAAVRRPDKEIVFQER
                     DYIPLTKRYKFMGLMFIRGTINLFEMMIIGIKCLMFSAEVALSEEERKPKGWEMSLSF
                     IISFAIAIFFFVVVPAFFFTKIKFFVSNLILLNILEGCVRLGIFLCFLCSTLLMEDMR
                     RVYMYHGAEHKTVFAWEHGQELTLENIKYFSTRHPRCGTSFILFVMIISILVFSLLGR
                     PDFLHRVIYKILLLPVVAGISYEAIRFTGKHSHWKWVQLLSWPGLMLQKITTREPSDD
                     QIEVAIAAMKKVI"
     gene            1768..>2593
                     /locus_tag="VF305_00175"
     CDS             1768..>2593
                     /locus_tag="VF305_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7415585.1"
                     /translation="MIKRYALALIVIIGSAALLYQQAAAKEDTAQITLKKTAVSRKNL
                     YTYTVKKGDILSAVVRNIPGITEQDISNNYQLIKELNPDLDDINELNPGQLLVLPGKS
                     LTEEKKVTTATAIPSAEAVAGATVYTIKKGDSLIKIIYRELGIKTNIQKTLNIIKSMN
                     PNIVDVNKIYTDAIINLPGKTLFVSAPEETKPVEQEVVKLAEETTPPEKIIEVKEKKV
                     MPPEARIAVIKQVITQMNGSLITTGNYYLPIPKTGQVTIDCSKIPVIEFDDKTTVFL"
BASE COUNT          821 a          548 c          447 g          777 t
ORIGIN      
        1 ttcggttaaa ttcctctatc gccgccttaa tttctttgcc gaaaaaaagt tcttcggtta
       61 attcctgtac ggctttttct tcatcgacat caacatattt tttgttgaca taattcatat
      121 cgccggtacg ggcctcgggc aaatcatgaa ggacacacat ttttaaaact cgtagctcat
      181 caagagaatc atcaagctta caaagggcgt aaccgacaaa gacagttcgc aatacatgtt
      241 ccgcaaccga ttccttgcca ctacccagaa actgataacc actgcgcggc gttttgctca
      301 gcattcccac ttcaaacaaa aaatcagcaa ttttatccat tttattcctt tttcaatact
      361 tttatgcggt tggccatcat ttccgccgtc tttgtaatgc ttacctgcaa agaagcaatg
      421 ctatccggtc tcatacttac tatcttatcc agttgcgctt tccaatgctc caaaagagct
      481 atttcatttt ctcttaattt tttttcaaaa cgttcctgat actctttaat aaatgcgttg
      541 gatgaatcca ttgacgccat cttcaatcct ccttttttct aaaagcaaaa gcattatatc
      601 attgaaggtc catcttgcaa caatcaattt aaaaaatgat aatttgacac taccggtcaa
      661 atactataaa taggctccac aaaatttatg aaaacatgga tgaataaata tgcctgataa
      721 acctaatccc gaggatatag ccggcggaca agctctaatt gaaggcgtca tgatgcgaca
      781 cggcaataaa attgccgccg cggtgagaag accggataaa gaaattgttt ttcaggaacg
      841 cgattatatc cctctgacca aacgctataa attcatggga ttgatgttta tccgcggaac
      901 aatcaactta ttcgaaatga tgatcatcgg aataaagtgt ctaatgttct cggcagaagt
      961 tgctttatcg gaagaagaaa gaaaaccaaa aggctgggaa atgtctcttt cttttatcat
     1021 cagcttcgcc atagcgattt ttttctttgt tgttgttccg gcttttttct ttacgaagat
     1081 taaatttttc gtttccaact taattcttct gaatattcta gaaggttgtg tccggttggg
     1141 aatctttctt tgttttcttt gttccactct cttgatggaa gatatgcgcc gtgtttacat
     1201 gtatcatgga gcggaacaca aaactgtctt tgcctgggaa catggacagg aattaactct
     1261 cgaaaatata aaatattttt ccacccgtca tccacgctgc ggaacaagct ttattctgtt
     1321 cgtaatgata atctccattc tggttttttc tttgctggga cgtccggatt ttttacatag
     1381 agttatctat aaaattttgc ttcttcccgt tgttgccggt atttcttacg aggctatccg
     1441 ttttaccgga aagcacagtc attggaaatg ggtacagtta ttgagttggc ccggtctaat
     1501 gttgcaaaaa attaccaccc gagaaccatc cgatgatcaa attgaagtcg ccattgccgc
     1561 aatgaaaaaa gtaatttaac accaagttgc tcctaacaaa tgcaacatcc gcttccaaga
     1621 aaattgactt tttatcgata ttctcacctt gctggctttc taaattcatt gccatactat
     1681 ataaagattt gacttttttt gccaattgaa gtaattaaat actaacatat tcacattttt
     1741 cactctctta aacaggggga aaacacaatg attaagcgat atgctttggc cttgattgta
     1801 attattggtt cagcagcctt gctttatcag caagcggcgg caaaggaaga cacggcacaa
     1861 ataacactta aaaaaacggc tgtctccaga aaaaacttat acacctacac tgtaaaaaag
     1921 ggggatattc tgtcggctgt cgtcagaaat attcccggta taaccgaaca agacatatcc
     1981 aataattacc agcttattaa agaacttaat cccgatttag atgatatcaa cgaactcaat
     2041 cctggacaat tactagtcct tcccggaaaa tccttaacag aagaaaagaa agttactacg
     2101 gcaaccgcaa ttccttccgc tgaagcagtt gcaggagcaa ctgtttacac aataaagaag
     2161 ggtgatagtt taattaaaat catttaccgc gaattaggaa ttaaaaccaa tatccaaaaa
     2221 acacttaaca ttattaagtc aatgaatcca aacatcgtgg atgtaaataa aatttataca
     2281 gacgcaatta tcaacctgcc gggtaaaact ctatttgtca gtgctccgga ggaaactaaa
     2341 cctgttgaac aagaagttgt aaaacttgca gaagaaacaa ctccaccgga aaagatcatc
     2401 gaagtaaaag aaaaaaaggt catgccgccg gaagcacgca tcgctgtaat taaacaagtc
     2461 atcactcaga tgaatgggtc acttatcacc actggtaatt actaccttcc cataccaaaa
     2521 actggacagg tgacaattga ttgctccaaa attccggtaa ttgaatttga cgataagaca
     2581 acagtatttc tgg
//
LOCUS       DASVIM010000010         7809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993 k141_1541,
            whole genome shotgun sequence.
ACCESSION   DASVIM010000010 DASVIM010000000
VERSION     DASVIM010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VF305_00180"
     CDS             complement(<1..562)
                     /locus_tag="VF305_00180"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415586.1"
                     /translation="MKNKTIAINNLQLTANSAIKKNAIGLAVLSGLLLFFSFPKYGSG
                     FIVWVAFIPLFFALRKATSITQGLILGFITGVVSYIGIIYWITYVVVNYGYLPLYLGI
                     IIMLLLACYLSIYIALFAGGIVYFRKKISLYLVAPVLWVCLEYCKSYLLTGFPWENLG
                     YSQYLNLYLVQFADILGVFGLSLLIIV"
     gene            complement(552..1376)
                     /locus_tag="VF305_00185"
     CDS             complement(552..1376)
                     /locus_tag="VF305_00185"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX7415587.1"
                     /translation="MKIISKLFFWRTHKYIKMKIDAILVKAQQKGFIDSSSREMIENI
                     LEFTHILVREIMIPRTEIVAVSTEATIEEIIKEVIESRHTRIPVYRGTIDNIIGILNV
                     KDLLTFWSKQITKEDILTCLTKPYYIPETKNTPLLFYELKENKKHIAIVIDEYGGTSG
                     LVTLEDLLEEIVGEIHDEYESNSATEGIFSLPDGTLIIDGRTEIEKIEEYLNISLEKG
                     RYGTLSGLILNSTKRIPLTGEKFQIEGLEITIENADERSIKKVKIKKLKTNNQNEE"
     gene            complement(1401..2273)
                     /locus_tag="VF305_00190"
     CDS             complement(1401..2273)
                     /locus_tag="VF305_00190"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7415588.1"
                     /translation="MDYEGLIIRPPSEAYSLLLQVTVGCSHNKCTFCGTYRQKKFKIK
                     PLEQIKKDLQEARTFENVERVFLCDGDALIIPQPRLEEILKLTNDNLPAIKRIGTYAN
                     AKSILRKPVDELKKLKEMGLKIIYLGVETGNVELLQKIHKGVTYEQMVAAARRVQEAG
                     IALSVTILLGIGGLEKSVEHAMDTARILSDIDPDYVGALTLMLVPQTPLYEDYMAGRF
                     ILPDRFGYIRELYLIISNSNFTNCYFTSNHASNYLPIKAYLPREKEKVLKMISSVIND
                     KDMNRIRPEYLRGL"
     gene            complement(2423..2499)
                     /locus_tag="VF305_00195"
     tRNA            complement(2423..2499)
                     /locus_tag="VF305_00195"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2463..2465),aa:Asp,seq:gtc)
     gene            complement(2546..2621)
                     /locus_tag="VF305_00200"
     tRNA            complement(2546..2621)
                     /locus_tag="VF305_00200"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2586..2588),aa:Val,seq:tac)
     gene            complement(2699..5104)
                     /gene="lon"
                     /locus_tag="VF305_00205"
     CDS             complement(2699..5104)
                     /gene="lon"
                     /locus_tag="VF305_00205"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7415589.1"
                     /translation="MFEEKKLDDQEKMIVMPLLPLRDVVVFPHMIVPLFVGREKSIAA
                     LESAMKYEKGIFMVAQKNAKKDDPNQEDIYNIGTIGIIIQLLRLPDGTVKVLVEGKTR
                     GVIKEYLPNNDFFLVKVSEIEDIDDQNDVKKQALIRSIKESFELYLKLSKKIHVEMMG
                     TIAAIEDASKLADVVITHINVKLEDKQKIIEILNIGERLEAIYSLMLSEIEILQVEEK
                     IKRRVKKQMEKTQKDYYLNEQMRAIQKEMGEKDDFRNEIVELEKRLKQKKMSEEATKK
                     ARQEIKKLQMMAPMSAEATVVRNYIDWILDMPWSEKTENKHTLKESETILEEDHYGLK
                     KVKERIIEYLAVQTLVKKNKGPILCLVGPPGVGKTSIAKSVARATNRKFVRISLGGVR
                     DEAEIRGHRRTYIGAMPGKIIQSLKKAGSNNPVFCLDEVDKLSSDFRGDPSSALLEVL
                     DPEQNIAFSDNYLDVDYDLSDIMFITTANVLQTIPAPLQDRMEVIRIAGYTEQEKLQI
                     AKKFLLAKQMEANGLLAENIEFTNGALLRIIRQYTREAGVRNLEREIASICRKVAKEI
                     VSNGNKKKLVITSRSIPKYLSVPMYRHGETEGKNEIGLTIGLAWTEVGGELLAIEASI
                     MEGTGKMVMTGKLGDVMQESVQAALTYIRARAEQFGLAKNFYKKIDIHVHVPEGAIPK
                     DGPSAGIAMATSIASALMKKKVRADLAMTGEITLRGRVLPIGGLKEKILAAHRGNIKM
                     VIIPKDNEKDLVEVPQNVQNALEIVFVEHIDEVLAIAFVKDEENFGETITPEVPVSVQ
                     SVN"
     gene            complement(5123..6385)
                     /gene="clpX"
                     /locus_tag="VF305_00210"
     CDS             complement(5123..6385)
                     /gene="clpX"
                     /locus_tag="VF305_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX7415590.1"
                     /translation="MIKKSKDNRMGQGMLFCSFCGKMQSEVRKLVAGPTAYICDECIE
                     LCRYIVEEEEDKTIEKDSKNGLLQPKEIKKYLDQYVIGQDKTKKILSVAVYNHYKRLF
                     SNVDSDGIEIQKSNVLLIGPTGSGKTLLAKTLAKFLNVPFTIADATTLTEAGYVGEDV
                     ENIILSLLQNADYDVSRASKGIVYIDEIDKIAKKSGNPSITRDVSGEGVQQALLKIME
                     GTMANIPPKGGRKHPQQEFIQVDTTNILFICGGAFNDLENIISKRLGVNAMGFGAEIR
                     SKKEKRSGELLAEARAEDLLKFGLIPEFIGRLPVIATLDDLDEATLIRILVEPKDAII
                     KQYKKLFALENVTLKFTDGALRAVAKLSMERKSGARGLRSILENTMLEVMYDIPSQSD
                     IKECVISEDVVLKKEKPILIYEGKSESA"
     gene            complement(6369..6986)
                     /gene="clpP"
                     /locus_tag="VF305_00215"
     CDS             complement(6369..6986)
                     /gene="clpP"
                     /locus_tag="VF305_00215"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX7415591.1"
                     /translation="MVVEQDGRGERAYDIYSRLLKDRIIFLGTTIDDNIANLVVAQML
                     YLESEDPDKEIFFYLNSPGGNVSSGMAIYDTMQYIKSPISTVCMGQTASMAALLLAAG
                     KKGKRFALPHSRILIHQPLGGFQGQATDIDIQAKEILRLKDELNRILAELTGQPLSKI
                     INDTERDYFMSSEQAKKYGLIDEIIIRKPASVANTKKGELSDKEK"
     gene            complement(7001..>7809)
                     /gene="tig"
                     /locus_tag="VF305_00220"
     CDS             complement(7001..>7809)
                     /gene="tig"
                     /locus_tag="VF305_00220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX7415592.1"
                     /translation="FFGSHNGESPQELKADNFLLELGSKSFIPGFEEQLVGMKNGETK
                     AINVTFPEDYHEKKFAGKEVTFTVTIKNLKEKILPEIDDNFIKNFDKYNSLDDLKNDV
                     RKSLEQECQRIEETNLQDKIMEIIHKENDFEVPPSLVEKQIFYMMADTQKRMTSAGMD
                     EKNAMELTFKMHDKFKDDATKVVKSFLLLKKIAEKESFIAEENDINKYIQKLADKHGR
                     DYELLKKTYESEENRDNLKVELIQKKVFDFIEQSANIKVIEKNGMDLEVK"
BASE COUNT         2142 a         1714 c         1324 g         2629 t
ORIGIN      
        1 ccacgataat taaaagagat aacccgaaaa cacctagtat gtcggcaaac tgaacgagat
       61 ataaattaag gtattgcgaa taaccaagat tttcccaagg aaagccggtc aaaagatacg
      121 acttacaata ctcaagacaa acccataaaa ctggcgccac taaatataag gaaatctttt
      181 tccggaaata gacaatgccc ccagcaaata atgctatata aatactcaaa tagcaagcca
      241 ggagcaacat aatgataatt cctagatata acggcaagta tccgtaattt actacaacgt
      301 aagtaatcca gtaaattatt cctatatagc tgacaacgcc ggtaataaaa cctaaaatca
      361 atccctgggt gattgaagta gccttgcgta gtgcgaaaaa aagtggaata aaagctaccc
      421 agacaataaa gccggaacca tactttggaa atgagaaaaa taacagcagg ccgctaagaa
      481 ctgccaaacc aatagcattc ttttttattg cgctattggc agttaattgg agattgttta
      541 tggctatagt tttattcttc attttggttg ttggttttga gtttcttaat tttgactttt
      601 ttaatactac gctcgtcggc attttcaatt gtaatttcca atccttctat ttgaaatttt
      661 tctcctgtca aaggaattct ttttgtcgaa tttagtatca aaccgctgag tgtaccgtat
      721 ctgccttttt ctaagctaat atttaaatac tcttcaattt tttcaatctc cgtccggcca
      781 tcgattatca gtgttccatc cggtaatgaa aagattcctt cggttgcaga attagattca
      841 tattcatcat gaatttcgcc gacaatttct tcgagcaggt cttccaatgt aactaaaccg
      901 gaagttcctc catattcatc aatgacaatc gctatatgtt ttttattttc ttttaactca
      961 taaaacaata aaggagtgtt ttttgtttcc ggaatgtaat acggcttggt tagacaagtt
     1021 aaaatatctt ctttggtaat ttgttttgac cagaatgtaa gcaggtcttt gacatttaag
     1081 ataccgataa tattatcaat tgttccgcga tagacgggaa tgcgtgtatg ccttgattca
     1141 attacttcct tgatgatttc ctcgatagta gcttctgtgc ttacagccac aatttccgtc
     1201 cgtggaatca ttatttcacg aactaaaata tgggtgaact ctaaaatatt ttcaatcatt
     1261 tctctcgaag aagaatcgat gaagcctttt tgttgcgctt tcacaaggat agcatcaatt
     1321 ttcatcttta tatatttatg agttcgccag aaaaaaagct tggaaataat tttcattatg
     1381 cttaaaatga atctccctaa ttataaaccg cgtaaatatt ccggtcttat ccggttcata
     1441 tctttatcgt taattactga actaatcatt tttaaaacct tttctttttc ccgtggcaaa
     1501 taagctttta tcggtaaata gttggaagca tgattggaag tgaaatagca atttgtaaaa
     1561 ttagaattgg aaattatcaa ataaagttcg cgaatataac caaatctatc cggtaaaata
     1621 aagcggccgg ccatgtaatc ctcgtataat ggagtctgcg gaacgagcat caaagtgagc
     1681 gcgcccacat aatccggatc gatatcgctc aaaattcggg cagtatccat cgcgtgttca
     1741 acgctcttct ctagccctcc tattcctaat agtatagtta cagagagtgc tatgcccgct
     1801 tcttggacac ggcgcgctgc cgcgaccatt tgctcgtaag ttacaccctt atgtattttt
     1861 tgcagtaatt caacatttcc cgtttccacg cctaaataga ttattttcag acccatctct
     1921 tttaattttt taagctcatc aacaggtttt cttaaaatgc ttttggcatt ggcatatgtt
     1981 ccgattcgtt tgatagctgg caggttgtca ttagttagtt ttaatatctc ttccaggcgt
     2041 ggctgcggta ttatcagggc atcgccatcg cataaaaaca ctctttccac attttcgaaa
     2101 gtgcgggctt cctgcaaatc cttcttaatt tgctccaggg gttttatttt gaatttcttt
     2161 tggcggtaag ttccacaaaa tgtgcattta ttatgtgaac aaccgacagt aacttgtaat
     2221 agcaggctat aagcttcact gggcggcctt attattaatc cttcataatc cataattgat
     2281 tcttttccct ctggccatcc taatttttta tggaagttaa taatttgttt agtgtttata
     2341 actttatatg aacaacatct aaaaagcaag tccttggaca aaaaaaggga gtcgccataa
     2401 cagctgactc cctttgattt tctggcgggg ccgatgggac ttgaacccac gccctccggc
     2461 gtgacaggcc ggcgttataa ccaacttaac tacgacccca aaaaatcatt ataattttaa
     2521 ttacagttcc ttgaattaac attaatggta ggcggaacag gactcgaacc tgtgacatcc
     2581 acggtgtaag cgtggcgctc tcccagctga gctacccgcc cgcaattcct caaaaacgct
     2641 gtgaggcttc tagcaacatt gacatttatt gtcaagttct aaaagtatag aaaacctttt
     2701 aattcacaga ttggacactg accggtactt ccggcgtaat tgtttcacca aaattttctt
     2761 catctttgac aaacgcaata gctaagactt catctatatg ctcgacaaaa actatttcca
     2821 gggcattttg cacattttgc gggacttcca ctaaatcttt ttcattatct ttcggaataa
     2881 tgaccatttt tatgttaccc cgatgagcgg ctagaatctt ttcctttaat ccacctatcg
     2941 gtaaaactct gccccttagt gtaatttcac ccgtcatggc taaatcagct cgtacttttt
     3001 tcttcatcag tgccgaggca atagaagtag ccatagcaat gcccgcggaa ggaccatctt
     3061 taggaattgc cccctccggc acatgtacat ggatatcaat tttcttataa aaattcttgg
     3121 ctaggccgaa ttgttccgcg cgagcccgaa tataagtcaa cgcggcctga acagattcct
     3181 gcataacgtc gcctaatttt ccggtcatta ccatcttgcc tgtaccttcc atgatggaag
     3241 cttcaatagc caatagttct ccgccgacct ctgtccaggc taaaccaatt gtcaaaccaa
     3301 tttcattctt gccctctgtt tccccgtggc ggtacatggg tacgcttaaa tatttgggta
     3361 tagacctgga agtaataact aattttttct tgttgccgtt gctgactatt tctttagcaa
     3421 ctttgcggca gatggaagca atttcacgtt ccaggtttcg cacaccggct tcccgcgtgt
     3481 attggcgtat aattctaagc aatgctccgt tggtgaattc aatgttttcg gccaataaac
     3541 catttgcttc catttgcttc gcgagcaaga atttttttgc tatttgcagt ttctcttgtt
     3601 ctgtgtatcc ggcaatccga attacttcca ttctatcttg cagaggagct ggaattgtct
     3661 gtaaaacatt tgccgttgta atgaacataa tatctgataa atcgtaatcg acatcgagat
     3721 aattatcact aaaggcgata ttctgctccg gatcaagaac ctccagaagc gctgaggagg
     3781 gatctcctct gaaatcggag ctgagcttat ccacttcatc cagacagaaa acgggattgt
     3841 tggaaccggc cttttttaaa gactggataa tctttcctgg catagcccct atatatgtgc
     3901 ggcgatggcc gcgaatctct gcttcgtcgc gaacgcctcc tagagaaata cgtacaaact
     3961 tcctgtttgt ggctctggcc actgatttgg cgatagatgt tttacccacc ccgggaggac
     4021 caaccagaca caaaatcgga cctttatttt tctttaccaa tgtttgaacg gcaagatatt
     4081 caataatgcg ctccttaacc ttttttaatc cgtagtgatc ttcttccaga atagtttccg
     4141 attctttcaa ggtatgttta ttttccgttt tttccgacca cggcatatct aatatccagt
     4201 caatataatt tctgactact gtagcctcgg cggacatagg agccatcatt tgtaattttt
     4261 taatttcctg ccgggctttt tttgttgcct cttcggacat ctttttttgc tttaaacgtt
     4321 tttccagttc gactatctca ttgcggaaat cgtctttttc tcccatttcc ttctgaatag
     4381 cccgcatttg ttcattgaga taataatcct tctgcgtttt ctccatctgc tttttaacgc
     4441 gtcgcttgat cttttcttcg acctgaagaa tctcgatctc agaaagcatc agtgaataaa
     4501 ttgcttccag acgttcgccg atatttaata tctctataat tttttgttta tcttccagct
     4561 tcacattaat atgggtaatt acaacatcgg caagcttgga ggcatcttca atagcggcaa
     4621 ttgttcccat catttccacg tggattttct tgctgagctt cagataaagc tcaaatgatt
     4681 ctttaatact gcgtattaac gcttgctttt tcacgtcgtt ttgatcatca atatcttcga
     4741 tctcacttac tttgaccagg aaaaaatcat tattcggcaa atattctttt atgacgccgc
     4801 gagttttccc ttcaactaaa actttgactg ttccatcagg caaacgtaaa agctgaatga
     4861 taatgcctat tgtaccaata ttataaatat cttcctgatt aggatcatcc ttcttggcat
     4921 ttttttgggc aaccataaaa atgccctttt catatttcat tgctgattcc agcgcggcaa
     4981 tagatttttc ccggccgaca aacaagggaa caatcatatg tgggaaaacc acaacgtctc
     5041 tcagaggcaa cagcggcatc acaatcattt tttcctgatc atctaatttt ttttcttcga
     5101 acatattttt cccatatatt tcttaagcag attcagattt tccttcgtaa attaaaatcg
     5161 gtttttcttt ctttaaaacc acgtcctcac taatgacgca ttccttgata tcgctctgag
     5221 aaggtatatc atacatgact tccagcattg tattttccag tatggagcgt aggccgcgcg
     5281 cacctgattt tctctccatt gataacttgg ctacagcacg tagagcaccg tccgtaaatt
     5341 tcaaagtcac attttccaag gcaaataatt ttttatactg cttgataata gcgtccttgg
     5401 gttccactaa aattcggatc aaagtcgctt catccaaatc atccagtgtg gcaataaccg
     5461 gtaaacggcc gatgaattcg ggtattaatc caaacttcaa taaatcttcc gcccgcgctt
     5521 cggccagtaa ttcaccggat cttttttctt ttttacttct aatttcggcg ccaaagccca
     5581 tcgcgtttac tcccaatcgc ttggaaatga tgttttctaa atcattaaac gctccgccgc
     5641 atataaataa aatatttgtt gtatccacct gaatgaattc ctgctgggga tgcttgcggc
     5701 cgcctttggg cgggatattg gccattgttc cttccataat ttttaataac gcctgctgaa
     5761 cgccttcacc ggaaacgtct ctggttatgg aaggatttcc tgatttcttc gcaattttgt
     5821 ctatctcatc gatataaaca atgcctttgg atgctctgct aacatcataa tcggcgtttt
     5881 gcaacaaact taaaataatg ttttccacgt cttctccgac gtagccggcc tcagttaaag
     5941 ttgtagcatc ggcgatagtg aaaggaacat tgaggaattt cgccagtgtt ttcgccagga
     6001 gtgtttttcc cgaacctgta ggcccgatta gaagcacatt acttttttga atctctatac
     6061 cgtcggaatc aacgttggaa aacaatcttt tgtaatgatt ataaacggca actgaaagta
     6121 tcttcttggt cttatcctga ccaattacgt attgatccag atatttcttg atttcttttg
     6181 gttgcagcaa accatttttg gaatctttct ctattgtctt atcttcttct tcctcaacaa
     6241 tataacggca aagctcaatg cactcgtcgc agatatatgc tgtaggaccg gcaaccagtt
     6301 ttcgcacttc gctctgcatt tttccgcaaa aagaacaaaa aagcatgccc tgccccatcc
     6361 gattatcctt acttttcttt atcacttaat tctccttttt ttgtattcgc cacggatgcc
     6421 ggcttgcgaa ttataatttc atcaattaaa ccgtattttt tagcttgttc actactcata
     6481 aaataatcgc gttccgtatc gttgataatc ttggaaagcg gctgtcctgt taattcggct
     6541 aagattctat ttaactcatc ttttaatctt aatatttctt ttgcctgaat gtcaatgtcc
     6601 gttgcctgcc cctgaaagcc tcccaatggt tgatggatta aaatacgtga atggggtagc
     6661 gcaaatcgtt tccctttttt ccctgcggcc aagagcagag ctgccatgct tgctgtttgt
     6721 cccatgcaaa cagtcgaaat cggtgactta atgtattgca tcgtatcata aatagccatg
     6781 cccgaactta cattaccgcc gggagaattt aaataaaaaa agatttcttt atccgggtct
     6841 tccgattcta aataaagcat ctgcgcgaca accaaattgg ctatattatc atcaatagtt
     6901 gtacccagaa aaattatacg atcttttaaa agacgtgaat agatgtcgta cgctcgttca
     6961 ccccggccat cttgttcaac caccatagga atcaaattca ctatttaacc tccagatcca
     7021 taccattttt ttcaatgacc ttaatattgg cactttgttc gataaagtca aatacctttt
     7081 tttgaattaa ttcaaccttg agattatccc tattttcttc actttcatat gtttttttca
     7141 ataattcgta atcccgtcca tgtttgtcag ctaatttctg aatatatttg tttatatcat
     7201 tttcctcagc aataaatgat tctttttcgg caatcttttt taaaagcaaa aaagatttga
     7261 cgactttcgt tgcatcatct ttaaatttat catgcatttt aaaggtaagt tccatcgcat
     7321 ttttctcatc cataccggca gaagtcattc ttttttgcgt atcagccatc atataaaaaa
     7381 tctgcttttc aacgagtgat gggggaactt cgaaatcatt ttctttatgt ataatttcca
     7441 tgatcttgtc ctgcaaatta gtttcttcta tgcgctgaca ttcttgttcc aaagatttac
     7501 ggacgtcatt ttttaaatcg tctaaagaat tatacttatc aaagttcttg ataaaattat
     7561 catctatttc aggcaatatt ttttctttta aattctttat tgttacggtg aaagtcacct
     7621 ctttccccgc aaattttttc tcatgataat cctcgggaaa agttacatta attgctttcg
     7681 tttcgccatt tttcattcct accagttgtt cctcaaagcc aggaataaag ctctttgatc
     7741 ctagttccaa cagaaaatta tcagctttta attcctgggg tgattctcca ttatgactgc
     7801 caaaaaaat
//
LOCUS       DASVIM010000011         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1537284, whole genome shotgun sequence.
ACCESSION   DASVIM010000011 DASVIM010000000
VERSION     DASVIM010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537284"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VF305_00225"
     CDS             complement(<1..832)
                     /locus_tag="VF305_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7415593.1"
                     /translation="MNSEALTDEKNIRRLRLLIISRVIIVTLFLSIAIYIDIKRQTFS
                     ASQRTINLLYLIAAATYLFSVTYALLLKFVKDLRPNIYLQLSIDILFVTFLVYITGSI
                     GSNYSLIYTLIIIYSVIFLGRKGGLIVASASGIFYGLLLDLEFYMLIPPISSVERDYT
                     LNAGDVFVRILVHIVSFYILAFLASFVVEQEKKTRSLLEEKESAFNQLDLLFRSIIES
                     VDTGVMTINLQGTIKTFNRAAEEITGFPLAGVENRRIADVFPEFVQFFAMEKIDEQIR
                     N"
     gene            947..3427
                     /locus_tag="VF305_00230"
     CDS             947..3427
                     /locus_tag="VF305_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEX7415594.1"
                     /translation="MRRPLIYPLIAFIAGIIASSYFAVPSYLLFAGIPLIIFFLILTS
                     RKKWSFASFILVIIFSFFLGSFAIQKHQYLVRAEKHISKYINAGKLTLEGLVIENPIS
                     YTDKNVLIVRCLRQIKDKSYEPVSGDIRLVIPSDLNFQYGDFIRFHSALKKIQSFQNP
                     GGFDYERYMNLQGIYATGFIANSSNIILLRQNTASGIRLKLETFRLYLKQIIYQNAST
                     PQREIIEAMTIGNQNAIPADVRDNFNKTGTSHILSISGLHIGMVAATIFFIISLILKS
                     SEYLMLRFNIIKLASAAAFLMVLIYALIAGMGVTVIRSALMALVFLIALFLGRQKDIF
                     NALALTALIILVISPEALFDISFQLSFTSVLAIIYIVPRFSNFSFPLLASLPVWWQKI
                     IRHIFLSILVCIAATLGTIPLIVFYFNRVSAITIIANLIAVPLLGTIALVFSMAFILG
                     AFFSPVIAGFFIKISSFFVQISVYIIDFLASLSWSSFSFTKPNFLEIFIFYLFVFLLV
                     QFIDMRKNKERKKGFLDRYPLTLKYLLIISLVLIIANGIYLLSKDKFSNDLKITAIDV
                     GQGSSTLVRFPGGVNMLIDGGGFTDSSFDMGKLVIAPFLYHERISTIDTVVLTHPHPD
                     HLHGLLYITENFNVKEVWSTGQLSEDEIFQQWQKIIAQKKIKINLISAQSPTAKINGV
                     QLNVLWPLKPPAINSDNLSDNDLNDTSLVLKIKYGNFQFLLPADISSNTEKTLIQRGS
                     NLKSDVLFVPHHGSAHSSCADFIKKVSCRYAVISAGKANVFHHPHPSTLEQYKSAQVQ
                     IFRTDKDGAITLTTDGIKLNIDTFRKSR"
     gene            3522..>4575
                     /gene="hisS"
                     /locus_tag="VF305_00235"
     CDS             3522..>4575
                     /gene="hisS"
                     /locus_tag="VF305_00235"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX7415595.1"
                     /translation="MEKITAIKGIKDILPEDSPAWNNIESIARQVFGSFGFREIRVPI
                     MEKTELFKRSIGETTDIVEKEMYTFRDRDDELLTLRPEATASIIRAYIEHNMQAYDQI
                     TKLFTIGPMFRREKPQKGRFRQFHQIDVELFGDEKPQSDAEVIFMLMHFLQSTSIEGL
                     SLEINSLGCKTCRPVFSQAIVNHLKGSKNALCPDCQRRMNTNPLRVFDCKVETCNSVI
                     AKAPQILDYLCPDCKEHFTQVKSYLTDLNVAYGINPKMVRGLDYYTRTAFEVKSGHLG
                     AQNALAGGGRYDGLVSFLGGPEVSGIGFAVGVERLLACLPNNEKNIFRTDLFVAALGE
                     RAQKVAFRLTNELRRAG"
BASE COUNT         1333 a          889 c          856 g         1497 t
ORIGIN      
        1 ggtttctaat ttgttcatcg atcttttcca tggcaaagaa ttgtacaaac tccggaaata
       61 catctgctat tctacgattt tcgactccag ccaatggaaa tccggtgatt tcttctgctg
      121 cacgattaaa agttttaata gtgccctgca aattaatggt catgacgccg gtatcgaccg
      181 actcaataat acttcggaaa agtaaatcca gttgattaaa tgccgattct ttttcttcca
      241 ataaagatct tgttttcttt tcttgctcga cgacgaaact ggctaaaaaa gctaaaatat
      301 aaaacgatac aatatgtact agaattcgaa caaatacatc tccggcgtta agggtataat
      361 cacgctcaac tgaagaaatg ggagggatta acatgtaaaa ttcaagatct aaaagtaatc
      421 cataaaatat gccggaggct gaagcgacaa tcaagcctcc tttacggccc agaaagatta
      481 ccgaataaat tattatcaaa gtgtatataa gcgaataatt actaccgatg ctgccggtga
      541 tgtaaactag aaaagtgaca aaaagaatat ctattgatag ctgtaaataa atattaggcc
      601 gtaaatcttt tacgaacttt aaaagcaagg cataagtaac cgaaaataaa taagttgcgg
      661 cagctattaa gtaaagaagg ttaatggttc tttgcgatgc agaaaatgtt tgtcttttaa
      721 tatcgatata aatagcaata ctaagaaaca gcgttacgat gataactctg gatatgatta
      781 aaaggcgcaa ccgccgtata tttttttcgt ctgttaatgc ttcgctattc actgtaatct
      841 tttagattcc tttttagcgt aatatgttta tgatcatgtt ataatatttt agcactttta
      901 gctaataaat ttgaactatt ttcccagaag agaaaaatac taaaatatgc gcagaccatt
      961 aatttatcct ttaattgctt tcatcgctgg aattattgcc agtagttatt tcgccgttcc
     1021 ctcttattta ttatttgcgg gaataccttt aattattttt tttctcattc tcacaagcag
     1081 aaagaaatgg tcattcgcca gtttcattct ggttataata ttttcttttt ttcttggttc
     1141 tttcgccatt cagaaacatc aatatttagt tcgggctgag aaacatataa gtaaatacat
     1201 taatgcgggc aaactaactt tggaaggcct tgttattgaa aatcctattt cttatactga
     1261 taaaaatgtt ttaattgtga ggtgtctgcg gcaaattaaa gataaatcat atgagccagt
     1321 ttcaggtgat attcgcctgg tgattccttc tgatttaaat ttccaatatg gtgattttat
     1381 tcggtttcat tctgctttaa agaaaataca aagctttcaa aatcccggtg gttttgatta
     1441 tgaacgatat atgaatctcc aaggtattta tgctaccggt tttatcgcca atagttcgaa
     1501 tattatactt ttgagacaaa ataccgccag tggcatcaga ttaaaactgg aaacattcag
     1561 actttattta aaacagataa tttaccaaaa cgcttctact ccgcaaagag aaatcattga
     1621 agccatgact atcggaaatc aaaacgcgat tccggctgat gtgcgtgata attttaacaa
     1681 gacgggaact tctcatattc tttctatttc cggcctgcat atcggaatgg ttgccgctac
     1741 tatttttttc attatttcgc tgattttaaa gagttctgaa tatctgatgc tgagattcaa
     1801 tattatcaag ttagcctcgg cagcagcttt tttaatggtt ttaatttatg ctctgatcgc
     1861 gggaatgggt gttacggtaa ttcgttcagc tctcatggcg cttgtcttct taatcgcatt
     1921 gtttctgggc agacaaaaag atattttcaa cgccctggca ctgacggcat taattatcct
     1981 ggtgatttcg ccagaggctc ttttcgatat ttcttttcag ctttccttta cgtcggttct
     2041 ggcgatcatt tatattgttc cgcgttttag taatttctct tttccgttat tggcgagtct
     2101 gccggtctgg tggcaaaaaa taatacggca tatattttta tcaattcttg tatgcattgc
     2161 ggcgactctg ggcactattc ctctgatcgt tttctatttc aaccgcgtct ctgcgataac
     2221 cattattgcc aatctcattg cggtgccact tctgggaaca attgcactgg tattttccat
     2281 ggcttttatt cttggcgcgt ttttttctcc agttatcgcc ggtttcttta tcaaaatatc
     2341 ctcattcttt gttcaaatct ctgtctatat tatcgatttt cttgcttcac tatcctggtc
     2401 atcttttagt tttacaaagc ctaatttctt ggaaattttc attttttacc tattcgtttt
     2461 tcttttggtg caatttattg atatgcgcaa aaataaagaa cgcaaaaaag gattcctaga
     2521 tcgctatccc ttaactttaa agtatcttct tattatttct cttgtcttaa ttatagctaa
     2581 tggcatttat cttctcagca aagataagtt ttctaacgat ttgaaaatta cagcaattga
     2641 cgtaggacaa ggctcttcca ctttagtgag atttccggga ggggtaaata tgcttatcga
     2701 tggcggcggg ttcaccgata gttccttcga tatgggcaaa cttgttatcg ctccttttct
     2761 ttatcacgaa agaatcagca ctatcgacac ggtggttctt acccatccgc atcctgatca
     2821 tctccatggg cttctttaca ttacagagaa tttcaacgta aaggaggtgt ggagcaccgg
     2881 acagctatcg gaagacgaaa tttttcagca gtggcaaaaa ataattgcgc agaaaaaaat
     2941 taaaattaat cttatatcgg cgcaatcacc cactgcaaaa ataaacggtg tgcaattgaa
     3001 tgttttatgg cctttgaagc ctccggcaat aaattctgat aatttatcag ataatgatct
     3061 caatgataca tctttggtgc ttaaaataaa atacggtaat ttccagtttc tgcttccggc
     3121 tgatatatct tcaaatactg aaaaaacgct cattcaaagg ggaagtaatt taaaaagcga
     3181 tgtccttttc gttcctcatc atggttcggc acactccagt tgcgctgatt ttatcaagaa
     3241 agtatcttgc cggtatgctg taatcagcgc cggaaaagcc aatgtcttcc accatcctca
     3301 tccctccact ctggaacaat acaaatcagc acaagttcaa attttccgca ctgataaaga
     3361 tggagcgata actctaacga ccgacggcat aaaattgaac attgatactt ttcggaaaag
     3421 cagataagta cttgttgatt aaccattttt tttcattgat aaccagcata ctatatgcta
     3481 gttaacttca ttaaatttca ttaaattttg gtctgatatt tatggaaaaa ataaccgcaa
     3541 ttaaaggaat caaagatata ttgcccgaag attcccctgc ctggaataat atagaatcaa
     3601 ttgcccgtca ggtttttggt tctttcggtt ttcgtgaaat tcgggtgccg attatggaaa
     3661 aaaccgaatt gtttaaacgg agtattgggg aaacaacgga tatcgtcgaa aaagaaatgt
     3721 acacttttcg tgatcgcgat gatgaactct tgacattgcg ccctgaagcc acggcttcca
     3781 ttatccgcgc ttacattgaa cataatatgc aggcttatga ccagataaca aaacttttta
     3841 caatcgggcc catgtttcga cgggaaaaac cgcagaaagg ccgtttccgt caattccacc
     3901 agattgacgt tgagttgttt ggcgatgaga agcctcaatc cgatgcggaa gttattttca
     3961 tgctgatgca ttttctgcaa tcaacgtcta ttgaagggtt atctctggaa attaactctc
     4021 tgggctgcaa gacttgccgg cctgtttttt cacaagctat cgtcaatcat ttaaaaggat
     4081 cgaaaaatgc tctttgtccc gattgtcaaa gacgcatgaa caccaatcct ttgcgggtat
     4141 ttgactgcaa agtagaaacc tgtaattcag ttatcgccaa agctccgcaa attctcgact
     4201 acttgtgtcc tgactgcaaa gaacatttta ctcaggtaaa atcgtattta accgacttga
     4261 atgttgctta cggcattaat ccgaaaatgg tgcgcggctt ggattattat acaagaacgg
     4321 cttttgaagt taaatcagga catctgggag cgcaaaacgc attggccggc ggcggcagat
     4381 atgacggctt ggttagtttc ctgggcggac cggaagtatc cggcattgga tttgccgtag
     4441 gtgttgagcg tcttcttgcc tgtctgccta ataatgaaaa aaatatattc cggacggatt
     4501 tatttgttgc cgctctcggc gaacgcgcgc aaaaggtggc cttccggctg actaacgaat
     4561 tacgccgggc cggta
//
LOCUS       DASVIM010000012         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993 k141_87402,
            whole genome shotgun sequence.
ACCESSION   DASVIM010000012 DASVIM010000000
VERSION     DASVIM010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87402"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VF305_00240"
     CDS             complement(<1..501)
                     /locus_tag="VF305_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7415596.1"
                     /translation="MVIIKKFICLLSTAFTTGLLFMLMASTVIAAEPIKIGAVLPLAE
                     TAGKDASRSMQLAVKQINAAGGLLGRQLELIIVDDEMKPDKAVAAIEKLVNTDKVDII
                     VGGMDNATTLEVIPVLKKHTKVTVWMGASSKKIEDALEGQGWFFHLHAWDYQLGAYYE
                     KMWREVV"
     gene            1072..1566
                     /gene="mobB"
                     /locus_tag="VF305_00245"
     CDS             1072..1566
                     /gene="mobB"
                     /locus_tag="VF305_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX7415597.1"
                     /translation="MVTPIISIVGKSNSGKTTLLEKIIPELVKRGYRVATIKHDVHGF
                     DIDHEGKDSWRHKKAGAHITMISSPCQIVLIEDVGRDYTLDEIRGKYIKGVDIILAEG
                     YKGNPFPKIEVFRSELKRTLLCKKEDNLLAIAANIKLDIGVPCFDINDAQSLVDLIEN
                     TFLK"
     gene            complement(1881..>2988)
                     /gene="thrC"
                     /locus_tag="VF305_00250"
     CDS             complement(1881..>2988)
                     /gene="thrC"
                     /locus_tag="VF305_00250"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX7415598.1"
                     /translation="FSVPLEKVCPQAYVMRLDQGPTASFKDFAARMMARLMSHCRPQG
                     ERINILVATSGDTGSAVGEAFKGVAGIDVTILYPAEEVSLRQKKQLDTIGGNVHALCV
                     EGKFDDCQNLVKQAFADPELAKFNLSSSNSINFGRILPQIVYYIYAYAQLAAAEEQVI
                     FSVPSGNFGNALGCEYARRMGLPVAKLVMPTNENDEFPVFLESGRYNKVSPSRACISN
                     AMNVGHPSNLARFFELYGGTVDRAGNVHRYPNLEEMRRYIYSISVSDEQTRQTIKSVY
                     DRYHVVLEQHGAVGWAGLEDYLNKTSKKNIAICLETAHPAKFPEEIQKLLGLKLNLPE
                     SLKDIDRRPGNAVSMRNNYDDFSNYLQKKLKIKK"
BASE COUNT          903 a          650 c          569 g          866 t
ORIGIN      
        1 gacaacttct ctccacattt tttcataata cgcgcccagt tgataatccc aggcatggag
       61 atgaaagaac cagccctgcc cttccagggc atcttctatc ttttttgaag aagctcccat
      121 ccagacagtt acttttgtgt gtttttttaa tacaggtatg acttccaaag ttgtggcgtt
      181 atccataccc ccgacaatta tatcgacttt atccgtattg actaattttt ctatagccgc
      241 caccgcctta tcaggtttca tttcatcgtc aactattatt agctccagct gccgtcccag
      301 taaaccgccg gctgcattga tttgctttac agccagttgc atagaccttg atgcatcctt
      361 acctgctgtt tccgccaacg gtaaaactgc acctatttta atcggctcgg ccgctataac
      421 agtgcttgcc attaacataa aaaggaggcc agttgtgaaa gccgttgaca acaaacaaat
      481 aaatttttta attattacca taatccctcc tgaataaagc ttttattata gagtgcaaat
      541 aagaaacata ttaaatatta tcattctact ttatagaaca tgaaatatta atttgccaat
      601 ttattaataa ttttttgaga gtactttttt tcttacttac tgtttgcaac aagatcagtg
      661 gactaatgat cattaagtga acatttacgt taaacattga agcacttcca ctgcaagctt
      721 agagttatct tcaacttaca gcaattaagt agcaggaaaa gctttttctg tcaagggata
      781 taaagagcag aaaccatgct ttattttaat tgtgagtata aaaatactcc cggctaatta
      841 tccggccgct gcaaaggata accggcgact ggcgggaata atgcgcatct tcaaattggc
      901 aaagaaataa ttatatgtta tgaatcaaac agcgtacatc cctctgcact gccggcaaag
      961 taagtaaatg ggctaatgat aaaaaaaatc accttgggca ttaaagattt cctgcagctt
     1021 acagtgggca attttcagtt tcatcaattt ttgggataat tttaataaaa catggtaacg
     1081 ccaatcatat ccattgtcgg caaatccaac tccggcaaaa caaccttact ggaaaaaata
     1141 attcccgaat tagtaaaaag gggataccgc gtagctacaa tcaagcacga tgttcatgga
     1201 tttgacatag accacgaagg caaggatagc tggcggcata aaaaagccgg cgcccacata
     1261 acaatgattt catccccctg ccagatagtt ttgattgaag acgtcggccg cgattacacg
     1321 ttggatgaaa tcaggggaaa atatattaaa ggcgtagata taattcttgc cgaaggttac
     1381 aagggcaatc ctttccccaa aattgaagtt ttccgttctg aactaaagcg cacgctttta
     1441 tgtaagaaag aggataattt gttagcaatt gccgcgaata taaaactgga tatcggcgtt
     1501 ccttgttttg atattaatga tgcccaaagc cttgtcgatt taattgaaaa cacattttta
     1561 aaatagttat ccgcaaccgg aaaaacaaac ctgcatattt cgcttttacc gcagcatttt
     1621 gactcccctt tgtaattttt atcatttgat tatatcgcac cttttgacat atccgcttta
     1681 gtcatctcca aagagaatct ggaaaaagaa ggttacggtg aatatctaaa attatttgaa
     1741 aaaaacaaaa aataatttcg gagatttttg cgaaacaatt gccatagcta gcagattcag
     1801 ccgtctaaaa gcaaaaaatc tgattatcgg tgtgacaata aaaacatata aaaccgtaaa
     1861 agaagatcgc caaattatag ttattttttt atctttaatt ttttctgcaa atagttgctg
     1921 aaatcatcgt aattatttct catggatacg gcgttcccag gccgacgatc gatgtctttt
     1981 aaactttccg gtaaattcaa tttcaatccc agaagttttt gaatttcttc cggaaatttt
     2041 gccggatggg cggtttccag gcaaatggct atatttttct tcgaggtttt gtttaaataa
     2101 tcttccagac ctgcccagcc cacagcgcca tgctgttcta aaaccacatg ataccgatcg
     2161 taaacacttt tgatggtctg tctggtttgc tcatctgaaa cgctgatcga atatatatat
     2221 cgcctcattt cttccaaatt gggataacga tgcacatttc ccgctcgatc aacagtgccg
     2281 ccgtacaatt caaagaaccg cgccaaattg ctgggatgtc cgacattcat ggcgttggaa
     2341 atacacgctc gggaaggcga aactttattg tatctgccgg attccaaaaa gacggggaat
     2401 tcatcatttt cattagtggg cataacgagc ttagccaccg gcaagcccat gcggcgcgcg
     2461 tattcacatc ccagagcgtt gccgaagttc cccgaaggaa ccgagaagat cacctgttct
     2521 tcggcagcgg caagctgggc gtaagcgtaa atatagtaaa ctatctgcgg caaaatccgg
     2581 ccaaagttaa tcgaattgga ggaagacaaa ttaaatttag ccaactccgg gtcggcaaaa
     2641 gcttgtttga ccaagttctg gcaatcatca aatttccctt ctacacagag agcatggaca
     2701 ttaccgccga tggtatcaag ctgttttttt tgccggagac ttacttcttc ggccggataa
     2761 agaatagtaa cgtcaattcc tgccactccc ttaaaagcct cacccacggc actgcctgta
     2821 tcgccggatg tggccaccag gatattgatg cgttcacctt gggggcggca atgactcatt
     2881 agtctcgcca tcatcctcgc ggcaaaatct ttgaatgatg ctgtggggcc ttgatccaag
     2941 cgcattacgt aggcttgcgg gcatactttt tccaaaggca cagaaaag
//
LOCUS       DASVIM010000013         5913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_258406, whole genome shotgun sequence.
ACCESSION   DASVIM010000013 DASVIM010000000
VERSION     DASVIM010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258406"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VF305_00255"
     CDS             complement(<1..277)
                     /locus_tag="VF305_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415599.1"
                     /translation="MKKILTVIIAMLVCFTGCATMDKEKQDIIRSISFSPDGKKILFD
                     RRKDDKPYMIHVYDLITGELSAYRSPDGEDWHIARYSFDGKRVVFTIT"
     gene            complement(549..1931)
                     /locus_tag="VF305_00260"
     CDS             complement(549..1931)
                     /locus_tag="VF305_00260"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7415600.1"
                     /translation="MSSILVIDDDASIRETLDLYLTEEGYTVETAENGTEGLNKYVQN
                     PADVVILDIRLPDVDGFSVLEDLKEENENVKVIMVTAFHDMETTINAMKSGAVDYIHK
                     PVNVDALDIAIKKALKSLEMEKKIDGLLMEPSRNFRVGDIIGTGNEMREIFKTIGVVS
                     KSRTTVLIQGESGTGKELIAKVIHHNTSPTEPFIAVNCSAIVETLLESELFGHEKGSF
                     TGAIARKLGKFELARYGTVFLDEISEMSINLQAKLLRILQEMEFDRVGGKDKVKVNAR
                     IMAATNKDLKTLVKEGKFRDDLYYRLNIVSINIPPLRTRHQDIPHLIDYLLAKINLDL
                     HKKVIGVSGEMMEIFMNYNWPGNIRELENLLVRACVVAQGQVLGKSDFPELGKEETAK
                     VQANGLTEIFVSTEPGKLLTLDEVEERYIRKIIKETDKNKGEICEILGISRPTFERKL
                     EKYGITFERE"
     gene            complement(1995..4550)
                     /locus_tag="VF305_00265"
     CDS             complement(1995..4550)
                     /locus_tag="VF305_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7415601.1"
                     /translation="MRIWFASATVIILILLFALTVNHAREKDMVELFSRQQLAYAQNT
                     AARMADIFAQVEKNIVLFPRFDPQGKIPSAEIEHDFKILYSGRESTVNVVALFDKEGK
                     IKRIFPKGAFPDVNLTRHFNTVKYHKKQYLSLALAEKSRAAGIKQKTDWHLIVGYPLW
                     RQENIFSGAWIVSLSLAAIIEGSEKQTKDNRLGDLWLVDEQGNFIIHPDSAFIGKNIS
                     NLVKNINGSEIDFSSEQGNYLDSLVRQNAKKEKRSIIAYYPLQVGGEKWFVLVASPYS
                     NVILPVRKTFAYILFSAILLILVVVVASMSFAYKEGQRLRTKAEEKRLKEREDWQEKL
                     LREKKTIEGIIEGSPIPVFVINKEHEVILWNRACMELTGYSSEDMVGTDKHYIPFYSV
                     KRPVIADLIINNDIEGLNQYYGTKEVKKSEKVLGAYEATDYFENLGGHSRFLYFLAAP
                     IYDEKGEIIAAIETLQDVSRDEELTRSLREYAETLQNELTEKIELRREIEELYNYLQS
                     ILKSLPDKIYEMDENGIVNYMSRGLKKEGGLSSREFKGVHFLEFVAPADRDFVLSMWE
                     QARKGIYKPYEIEATAKDGRKLNLLVTTSPVIGTNRYIIVQRDITEFKNLEKKLYESQ
                     KLAALGQLSAGIAHEVRNPLSSIKMSLQILEKRMDPAGNDLKRFKIAEKEVEHLETLV
                     NNVLVFAKPVEPKKIPVDLAKVLDHAIAMAEKGIADKQIELQTEFSNIPPITVDAAMM
                     GDAFLNIIRNAIEAVEEQGNILVSLNYSDETHQSVFVEIKDNGCGINEEDMPHIFNPF
                     FTVKKYGTGLGLSQVKKIIDLHQGTIDVISEKGKGTKVYVTLPCYERRRVPRN"
     gene            complement(4671..4853)
                     /locus_tag="VF305_00270"
     CDS             complement(4671..4853)
                     /locus_tag="VF305_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415602.1"
                     /translation="MQEILTAVTYPIVWLICSYGLNIESGLAKLLISLIAAILVYVLI
                     GIKRSQKENRENKEGE"
     gene            5389..5766
                     /gene="atpE"
                     /locus_tag="VF305_00275"
     CDS             5389..5766
                     /gene="atpE"
                     /locus_tag="VF305_00275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX7415603.1"
                     /translation="MMSHKFSKKIITIFTAIIVLLVSSPLVFAAETITAGESYSKAIF
                     AVGAMIAAAFSIGVGAIGAALGIGSAANAACNAVGRNPGVQGKIMMTMLVGMAMAESI
                     AIYALVISLILLYANPYVRYFLG"
BASE COUNT         1665 a         1318 c         1064 g         1866 t
ORIGIN      
        1 gcgttatagt aaaaactacc ctcttcccgt caaaagaata tcttgcgata tgccagtctt
       61 ccccatctgg tgacctatag gcgcttaatt caccagttat aagatcatag acatgaatca
      121 tatacggctt gtcatccttt cttctatcga agagaatctt ttttccgtca ggtgaaaaac
      181 ttatacttcg tataatatct tgtttttctt tatccatagt cgcacatccc gtaaaacaaa
      241 caagcattgc tattattact gtaagaattt tcttcatcat cgcctcctgt ttcttgccgt
      301 tactatatgt aactccgatt acactaaagc ttggaacgta aatagactat cttcttgaaa
      361 tatcagtttt cattacggct cctaaataat atagcgctat gtggtcaatg aaaactaact
      421 cttttggtgc tgttcaaaaa tgcttagatg caaggcacgc gagtattgcg gagtgaggcc
      481 tactttttcg taggtcgcag cgacgcgaac ggagcgcaac acagcagatg agcgtttttg
      541 gacagccctc attcccgctc aaaggtgatg ccgtattttt ccaacttccg ttcaaaggtt
      601 ggccgggaaa ttcccaatat ctcgcaaatt tcccctttat ttttatccgt ttctttaatt
      661 atttttctga tgtagcgctc ctcaacttca tccaaagtta aaagtttacc cggctctgtt
      721 gatacaaata tttccgtcaa tccgttggcc tgaactttgg cggtctcttc tttacccaat
      781 tccgggaaat cgctcttgcc caagacctgg ccctgagcca ctacacaggc gcgcactagt
      841 aaattttcca attcgcggat attgcccggc caattataat tcatgaatat ttccatcatt
      901 tcgccagaaa cgccgatgac ctttttgtgc aaatccaaat taatttttgc cagcaaataa
      961 tcaatcaagt gaggaatgtc ttgatggcga gtgcgcagcg gaggaatatt aatagataca
     1021 atatttaagc ggtaatacaa atcgtcgcga aacttccctt ccttcactaa tgtctttaga
     1081 tctttatttg tcgccgccat tatgcgggcg ttaaccttga ccttatcttt gccgcctacc
     1141 ctgtcgaatt ccatttcctg taaaatacgc aggagctttg cctgcaaatt aatggacatt
     1201 tcactgattt catccagaaa gaccgtgccg taacgggcta actcaaactt gcctaacttg
     1261 cgagcaatgg cgccggtaaa tgagcctttt tcatgaccga ataattccga ttccagtagg
     1321 gtttcaacaa tggccgagca attgacggca ataaatggtt cggtaggaga tgtgttatga
     1381 tgaataacct tggcaattaa ttctttgccc gtgccgctct ccccttgaat aagaacggtt
     1441 gtccggcttt tcgaaacgac tccgatcgtt ttaaatattt cccgcatttc attgccggtg
     1501 ccgataatat caccaacccg gaaattgcgc gaaggttcca tcaaaagccc gtctatcttt
     1561 ttttccattt ccagggactt caaggctttt tttatcgcga tatctaacgc atcaacgttt
     1621 accggtttat gaatataatc gacggcgccg cttttcatgg cgttaatcgt tgtttccatg
     1681 tcatgaaaag cagtaaccat tattactttg acgttttcgt tttcttcctt caggtcttcc
     1741 aaaaccgaaa acccatcaac gtccggcagg cgaatatcca aaatcacaac gtcagcaggg
     1801 ttttgcacgt atttgttcaa accctccgtg ccgttttcag cggtttccac tgtgtaccct
     1861 tcttccgtca gatacaaatc aagggtctcg cgaattgaag catcgtcatc aataacaaga
     1921 atacttgaca taatcgttac cctgttttct gaatacaatt cgtccggcgc gacattacat
     1981 tacccacagg tggattaatt tcttgggaca cgccgccttt cgtaacacgg cagcgtgaca
     2041 taaacttttg tacccttccc tttctcacta ataacatcta tagtgccctg atgaagatca
     2101 attattttct tcacctgaga aagccctaaa cctgtaccat atttttttac cgtaaagaaa
     2161 ggattaaata tatgcggcat atcttcttca ttgataccgc atccgttatc tttaatttcc
     2221 acgaatactg attgatgagt ttcgtcagaa tagtttagcg aaactaaaat attaccttgc
     2281 tcttctactg cctctatagc gttacgaata atatttaaaa aagcgtcacc catcatcgcc
     2341 gcgtcaactg ttatgggcgg tatattacta aattctgttt gcaactcgat ttgtttatcg
     2401 gcgattccct tttcggccat agcaatggca tggtcgagca ctttggccaa atccactgga
     2461 atctttttgg gttctaccgg cttggcaaaa accaaaacat tattgaccag cgtctccaaa
     2521 tgctctactt ctttttcagc gattttaaat cttttcagat cattgccggc agggtccatc
     2581 cgcttttcca atatctgtaa actcatcttt atcgaagaaa gcggattgcg cacctcatga
     2641 gctattcccg ccgataactg tcctaaagcg gccagcttct gactttcgta aagctttttt
     2701 tccaagtttt taaattcggt gatatcacgc tggacaataa tataacgatt ggtgccgatg
     2761 acgggagacg tggttaccaa cagatttagc tttcgaccat cctttgccgt ggcttcaatt
     2821 tcatatggtt tataaatacc ttttctagcc tgttcccaca tagataagac aaaatcccta
     2881 tctgcgggcg cgacaaattc taaaaaatgt acgcctttaa attcacggga agatagtccg
     2941 ccttctttct ttaacccgcg gctcatataa ttgacgatgc cgttttcatc catttcataa
     3001 attttatcgg gcaagctttt taatattgac tgcaaatagt tgtagagttc ttcaatttcc
     3061 cggcgcaatt caatcttctc ggtcaactca ttttgcagtg tttcggcgta ctcgcgtaaa
     3121 cttctggtca attcttcatc gcgcgaaaca tcttgtagtg tttcaattgc cgctataatt
     3181 tccccttttt catcgtaaat aggagctgcc agaaaataca aaaaccggct gtggccgcct
     3241 agattttcaa aataatctgt cgcttcatag gcgcccaata ctttttccga tttttttacc
     3301 tctttagtac cgtaatattg attcaaacct tcaatatcgt tattaatgat caaatcggca
     3361 ataaccggtc ttttgactga gtagaaagga atgtaatgtt tatctgtacc gaccatatct
     3421 tcagacgaat aaccggtaag ctccatgcaa gcgcgattcc ataggatgac ttcgtgttcc
     3481 ttgttgataa caaaaacggg gatcggcgag ccttcaataa tgccttcaat ggttttcttt
     3541 tcccgtagaa gtttctcctg ccaatcttcc cgttctttta atcgtttttc ttctgccttt
     3601 gtgcgcagcc tttggccttc cttgtaagca aaagacatgc tggcaacaac tactacaaga
     3661 atcagtaaaa tagcgctgaa aagaatgtaa gcaaatgttt tgcgcacggg tagaatgaca
     3721 ttactgtatg gtgatgcaac caatacgaac catttttccc cgccgacctg taaaggataa
     3781 taagcaataa tgcttctttt ctctttttta gcgttttgcc gaaccaatga atccaaataa
     3841 tttccttgct ctgaagagaa atcgatctca gatccattta tattttttac caaattgctg
     3901 atgttttttc cgataaaagc agaatcagga tgaataataa agttgccttg ttcatctact
     3961 aaccaaaggt cacccaatct attgtctttt gtttgtttct cagaaccctc aattatcgcg
     4021 gccagagata gggatactat ccaagccccg gaaaaaatat tttcctggcg ccaaagggga
     4081 taaccaacaa ttaaatgcca atccgttttt tgctttatac cggctgcacg tgatttttcc
     4141 gccaaagcta aacttaaata ttgtttttta tgatacttta cagtgttaaa atgccttgta
     4201 agatttacat cggggaaagc acctttgggg aaaatacgtt ttatcttccc ctctttatca
     4261 aacagggcaa caacattaac tgtactttcc cgtcccgaat agagaatttt aaaatcatgt
     4321 tcaatttctg ctgaaggtat tttcccttga ggatcaaagc ggggaaagag gacaatattt
     4381 ttttcgactt gtgcaaatat atcggccatt ctcgctgccg tattttgggc ataggcgagc
     4441 tgctggcggc tgaagagctc aaccatatct ttttcacgag catgatttac tgtcaaggcg
     4501 aacagcaaaa tcaatatgat tacagttgca ctggcaaacc agatgcgcat cttcaatatc
     4561 cccaataatg tgatgtattt ttaacataaa tcgtaatgcc gaagaatgcc gcacaacacc
     4621 acctgaaggt ggggccaagc aggtggatta ttacaaagtc gtcaataata ttattcacct
     4681 tctttattct ctctgttttc tttttgggag cgcttaattc ctattaagac ataaaccaag
     4741 atagccgcta taagcgaaat aagaagtttc gccaaaccgc tttcaatgtt taaaccgtaa
     4801 gagcaaataa gccagactat cgggtaagta accgcggtca aaatctcttg catggcaatc
     4861 ctttaataag aaaatttatg gtattttaaa acacaccgcc gataattaat ttaatactat
     4921 cggcctcagc acaaaatttc aatagaaata taaaaaagta ttatccttat atattgtgaa
     4981 ataaatcgag tctgttggaa attactaaat atactttacc catcattctt gcatcatttg
     5041 aatgttttat atcggacgat tgaacaaaac tttaaattag aacaaaaatt gaaattagtt
     5101 tttagcatca taaaagaata atgagtaaat atttttatgc caggtcgcca tcatggcgcg
     5161 atcaatgaat gagcgcaatc tcaataaatt agctggctaa gccgctacac gccgcgcaat
     5221 aacaagcatc aatttagtat cttcgccata ccccttcccc gataatggca cacatcgtgc
     5281 aaatatctca actgttttga acgataatga ttccctcttt tcatggcatc taatttatgt
     5341 tattgacaga gacagattat tattaacaat taacgggaaa ggagaaaaat gatgtcacac
     5401 aaatttagta aaaaaattat aaccatattc actgcgataa tcgttctgct tgtatctagt
     5461 ccgcttgttt ttgccgcaga aactataaca gcaggtgaat cttactccaa agctattttt
     5521 gctgtaggcg caatgatagc cgcggctttt tctatcggag tgggagccat tggtgcagct
     5581 ctgggtattg gatcggccgc taacgctgcg tgtaacgctg taggaagaaa ccccggcgtc
     5641 caaggaaaga tcatgatgac catgttagtc ggtatggcta tggctgaatc aattgctatt
     5701 tatgccttgg ttatttctct aatacttctc tatgctaacc cttacgtgcg ttattttttg
     5761 ggttaattct tcacaagtta actaaaaaat taacaaaggt tagggggaaa ttataatgtc
     5821 agaaactaaa aaggataagc agttttttaa tataggaggc gtgttcttcc tgcttatcgt
     5881 aatcccctta tcactgatgg catttttaat tgc
//
LOCUS       DASVIM010000014         4166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1453134, whole genome shotgun sequence.
ACCESSION   DASVIM010000014 DASVIM010000000
VERSION     DASVIM010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453134"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="VF305_00280"
     CDS             <1..1439
                     /locus_tag="VF305_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HEX7415604.1"
                     /translation="PPAKGQELEDQYFGSIKERMSSYMHDMEEELFKLGIPAKTRHNE
                     VAPSQFELAPMFEEANLAVDHNQLVMDTMQSVAKKHNLAALLHEKPFAKINGSGKHVN
                     WSLSDNSGNNLLTPGNTPHDNIQFLVFLVAIIRAVYKHADILRASVATYANDHRLGAN
                     EAPPAIISIFLGEQLTNILSNIEKGIVTKATNAEIIDLDVSSIPIVSKDNTDRNRTSP
                     FAFTGNKFEFRAVGSSQSISFPTAVINTIVAESLDYLAEKIKAKGGNINQAVFEILKK
                     EIKDIKPILYNGDNYTKEWEIEAGKRHLPNDKTTPVALKALVTDKALNLFEKYQVLSK
                     VELKSRYLIYIEKYIKDLEIEVKCLNNICMNQVIPAASAYQKILAKCINETKKALGNS
                     AVLSAQIKILKNILEQINKVYSTNKEILSKVESASAIHDEAKKAEMLCSKVKTKMNEL
                     REYVDELEGIVDDELWPLPKFWEMLFIS"
     gene            1749..2891
                     /gene="carA"
                     /locus_tag="VF305_00285"
     CDS             1749..2891
                     /gene="carA"
                     /locus_tag="VF305_00285"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX7415605.1"
                     /translation="MPLLNKELRALLVLEDGGVFEGFAFAGSGETMGEVVFNTGMTGY
                     QEVITDPSYKGQIVTMTYPLVGNYGINEEDMESSGVHLEGFIIKEYQPNPSNWRMRQS
                     LKSFLEEHGKIGIEGIDTRALTRRLRLSGAMKGIISTKTKDIDLLLQRVKAYPGLVGR
                     DLVREVSCTKPYLWKNGTAHRGKISVTKPGKKLRVVVLDCGVKYNILRHLEKNGCEVI
                     VVPASSTGADIMSYKPDGILLSNGPGDPAALPYIVETSRALVGKLPIFGICLGHQIIG
                     QAIGGYTSKLKFGHHGINQPVKNMDTGRIEITSQNHGFVVVPESVKNKAKKTFDNLND
                     ATSEGMKMPLTMSVQYHPEASPGPHDTNYLFMQFVQMLRKKRRKAA"
     gene            2888..>4166
                     /gene="guaA"
                     /locus_tag="VF305_00290"
     CDS             2888..>4166
                     /gene="guaA"
                     /locus_tag="VF305_00290"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX7415606.1"
                     /translation="MILIIDFGSQYNQLIARRVRENHVYCQIEPPSISLSVIKSINPE
                     GIILSGGPASIYSKDAPRVDSGIFKLGIPVLGICYGLQYMADALGGRVERSAKREYGF
                     AELLVKEKKGIFKGVDKKTQCWMSHGDKIGQLPAGFVITASTENTIVAAAEDSKKHFY
                     GLQFHPEVVHTHSGRKILTNFLFDVCGCKKTWSMKSFANHAIAEIKSKVGSDKVILGL
                     SGGVDSSVAAVLLHRAIGKNLTCVFVDNGVLRESEAGRVIEMFKKHLHINLRFARSGK
                     LFLRRLKGVTDPEKKRKIIGRTFIEVFDCEAHKIKNVKFLAQGTLYPDLIESRSAFGG
                     PSAVIKSHHNVGGLPKKMNLKLVEPLKHLFKDEVRLLGKELGLADDVVWRQPFPGPGL
                     AIRIIGEVTPQRLSVLRKADTILLEEIRANKLYY"
BASE COUNT         1249 a          890 c          943 g         1084 t
ORIGIN      
        1 cacctccggc caaaggtcaa gagttggaag atcagtattt cggcagcatc aaggaaagaa
       61 tgtcttccta tatgcatgat atggaagaag agctctttaa gctgggtatc cctgcaaaaa
      121 caagacacaa tgaagttgct cccagtcagt ttgaattagc ccccatgttt gaagaggcta
      181 acttagcggt tgatcataac cagcttgtta tggacacaat gcaatcggtg gccaaaaagc
      241 acaatcttgc cgctttactc cacgaaaagc ccttcgcgaa aataaatggt tccgggaaac
      301 atgttaactg gtcgctctct gataacagcg ggaacaactt gttgaccccg ggcaatacgc
      361 cacatgacaa cattcagttt cttgtatttc tagttgcaat aattcgcgct gtctataagc
      421 atgccgacat cctccgggca tctgttgcca cctatgccaa tgatcacaga ctcggagcca
      481 atgaagcgcc gccggcaatc atatccattt ttcttggcga acagcttacc aatattcttt
      541 cgaacataga aaaaggaata gtgaccaaag ctacaaatgc cgagatcatc gatcttgatg
      601 tttcgtctat acctatcgtc agcaaagata atactgaccg caatagaacc tcaccgtttg
      661 cgttcaccgg taacaaattc gaattcagag ccgtcggttc atcgcagtct atttctttcc
      721 ccactgcggt aatcaacacg atagtggccg aaagcctgga ctatcttgcc gaaaaaatca
      781 aagccaaagg cggcaacatc aaccaggctg tattcgaaat attgaagaag gaaattaaag
      841 atatcaaacc aattctctac aatggcgaca attatacaaa agaatgggaa attgaagccg
      901 gcaaaagaca tttgcccaac gataaaacca cgcctgtagc actgaaagct ttagttactg
      961 ataaggcgct gaacctcttt gagaaatatc aagttctttc caaagtcgaa ctcaaatcca
     1021 gatatttaat ctacatagaa aaatacatca aagatctgga gatagaagtc aaatgcctca
     1081 acaacatttg catgaaccag gtaatcccgg ctgcatctgc gtatcaaaaa atacttgcca
     1141 aatgtattaa tgaaacaaag aaagcgcttg gtaattcagc tgttctttcc gcgcagataa
     1201 aaattttaaa aaacatttta gagcagatta ataaagtcta ttcgactaat aaagaaattc
     1261 tttccaaagt tgaatcggca tcggcaattc atgatgaagc gaaaaaagcc gagatgcttt
     1321 gcagcaaagt aaaaacaaaa atgaatgaac ttcgtgagta cgtcgatgaa ctggaaggca
     1381 ttgtagatga cgaattgtgg ccgctgccga aattctggga aatgttgttc atcagctagt
     1441 taattaaagg cttgtgtcag gatatcaccg gggacgatgc atgttcatga tattctgaca
     1501 ctacggccgg caacttcgat acttatgtca gcccatatga aattagcctc tattttcccg
     1561 tgcattaata attgacgaag ccggaaaaaa aagttaagaa gctccgtcat gtaaggtgca
     1621 ataaagggag cttcctatat ccagaaccaa ttatttgtcc actcctttcc tttttggtcc
     1681 aaatatagtc ttgattggtc tggaaaagac tcctccggat gttggtttag tcgggagata
     1741 agattttaat gcccctgtta aataaagagc taagagcatt gctggtcctg gaagacggcg
     1801 gtgtttttga aggttttgcc tttgcgggaa gcggcgaaac catgggcgag gtcgttttta
     1861 atacgggtat gaccggctat caggaagtca ttaccgatcc ttcctataaa ggtcagattg
     1921 tgacaatgac ctatcctctg gtaggcaatt acggcattaa tgaagaggat atggaatcgt
     1981 ccggagttca tctggaagga ttcatcatta aagaatacca gcccaatccc agcaactggc
     2041 ggatgcgcca aagcctgaaa tcatttctgg aagaacacgg gaaaatcggc atagagggaa
     2101 tcgatacccg tgccctgaca cgtcgcctgc gtctttccgg cgccatgaaa ggcatcattt
     2161 ccacaaaaac aaaagatatt gacttgcttc ttcagcgagt gaaggcatat cccggtcttg
     2221 tcggacgtga tttagtccgc gaagtaagct gcaccaaacc ttatctatgg aaaaatggca
     2281 cagctcatag gggtaaaatt tccgttacta agcccggcaa aaaattgcgg gttgttgtgc
     2341 ttgattgcgg tgttaaatat aatattttaa ggcatctaga aaaaaacggc tgtgaagtaa
     2401 ttgttgttcc cgcttcttct accggtgcgg atattatgtc gtataaaccc gacggtatct
     2461 tgctttccaa tggtcccggc gatccggcag ccctgcctta tattgtggag acttctcgcg
     2521 cattggtcgg gaagcttcct atctttggca tttgtctggg acatcaaata atcggtcagg
     2581 caattggcgg ctataccagc aagttgaaat ttggtcatca cggcattaat cagccggtaa
     2641 aaaatatgga tacgggacgt attgaaatca cgtcccaaaa tcacggtttt gtcgttgtgc
     2701 ccgaatcggt aaaaaataag gcgaaaaaaa ccttcgacaa tttaaatgat gcaacctccg
     2761 aaggcatgaa aatgcctttg actatgagcg tgcaatacca tccggaagcg tcacccggtc
     2821 cacatgatac aaattattta tttatgcagt ttgttcaaat gctgcggaaa aaaaggcgga
     2881 aagccgcgtg attttaataa ttgatttcgg ctcacaatat aatcagttga tcgcccgcag
     2941 ggttagagag aatcatgtat attgtcaaat cgagccgcct agtatttcac tttccgtaat
     3001 caagtccata aatccggaag gaataatcct gtcgggtggc ccggcgagta tttacagtaa
     3061 agacgcaccg cgggtggaca gcggcatatt taaactcggc attccggtat tgggcatttg
     3121 ttacggcctt caatatatgg ctgacgcttt aggcgggcga gtggaacgat ccgcaaaacg
     3181 agagtatggc tttgccgagc ttttagtaaa agagaaaaag ggaattttta aaggtgttga
     3241 taaaaaaact caatgctgga tgagccatgg cgacaagatt ggccaactac cggctggatt
     3301 cgtcattacc gcttccacgg aaaatacgat cgtagcggca gctgaagatt ctaaaaaaca
     3361 cttttacggt ttgcaattcc atccggaagt agtccatacc cactccggaa gaaaaatatt
     3421 aaccaacttt ttatttgatg tttgcggctg taaaaaaacc tggtcgatga aatcatttgc
     3481 caatcatgcc attgcggaga taaaaagtaa ggttggctcg gacaaagtaa tattgggcct
     3541 ctccggtgga gttgattctt ccgtggccgc tgttttactg cacagggcca ttggtaaaaa
     3601 cttaacctgc gtttttgtcg ataacggcgt tttgcgcgaa agcgaagccg ggcgcgtaat
     3661 agaaatgttt aaaaaacatt tacatataaa tttgcgtttt gcgcgttccg ggaagctgtt
     3721 tctccggcga ttgaagggag tgaccgatcc ggaaaagaaa cgtaagataa tcggccgtac
     3781 ttttatagag gtttttgact gcgaggccca taaaattaaa aacgtgaaat ttctagcgca
     3841 gggcactctt tatcccgatt taattgagtc gcgttctgct ttcggcggtc ccagcgccgt
     3901 cattaaatcg caccataacg tcggcggtct gcccaaaaag atgaatctta aacttgtcga
     3961 gccgctcaag catttattta aagatgaagt acggcttctg ggaaaagaac tgggcttagc
     4021 cgatgacgtt gtctggcggc agccgtttcc ggggcccggc ctggctattc gcattatcgg
     4081 cgaggttacc ccgcagagat tgtccgtatt gcgtaaagca gatacaattc tgctggaaga
     4141 aatccgcgca aataaacttt attaca
//
LOCUS       DASVIM010000015         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_345722, whole genome shotgun sequence.
ACCESSION   DASVIM010000015 DASVIM010000000
VERSION     DASVIM010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345722"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VF305_00295"
     CDS             complement(<1..639)
                     /locus_tag="VF305_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415607.1"
                     /translation="MLNYFWKKFSCIAGFVLVTLLTLSITAQAAEKGVEAKHPVRIAV
                     ISFQPVIPEEESGSTVICPICGIGYSSGKILKGSEKMVEEVFIDKLHELKEVAIIPPD
                     KVQGVYKRIASESLKKPLLKVLKIVGNELDADILAVGYIYRYTERVGYDYSSEHPASV
                     AFEIHLIKTIDGSIIWRGFFDKTQKSLMEDVFQISSFVRGGGKWMTARQLTEQ"
     gene            complement(658..1245)
                     /gene="hisB"
                     /locus_tag="VF305_00300"
     CDS             complement(658..1245)
                     /gene="hisB"
                     /locus_tag="VF305_00300"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX7415608.1"
                     /translation="MARRVKIIRKTTETNIQLEIDLDKAAGSKIDTTIPFFDHMLELF
                     ARHGFFKLIIKSKGDTQIDDHHLVEDIGICLGQAMGQALGKKMGINRYGSASVPMDEC
                     LCRVDLDISGRPYLIYNIKYERRKIGGFDPALVKEFFKAFTDHSGITLHINLAYGSNS
                     HHIIEAVFKAFARALGKAVTLNDNINGVLSTKGRL"
     gene            complement(1260..2132)
                     /gene="hisG"
                     /locus_tag="VF305_00305"
     CDS             complement(1260..2132)
                     /gene="hisG"
                     /locus_tag="VF305_00305"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX7415609.1"
                     /translation="MKVLKLGIPKGSLEENTVDLFKKAGWRITYDSRSYFPDVDDPEI
                     SCTLVRAQEISRYIEDGTLDLGLTGIDWILENSSDVVVVQDLIYSKVSMRQARWVLVV
                     REDSPYQKIDDMEGKKISTELVNFTKKYFAQKNINVHVEFSWGATEAKVVEGLVDAIV
                     EVTETGSTIKANKLRIIHELMKTNTQLIANRASWQDSWKRKKIEQIKTMLTGSLQAMG
                     KVGLKLNVSKENLGKVMTLLPSLKAPTISSLSSEEWFAVESIVDARIMRDLIPVLLEA
                     GAQGIIEYPLNKVI"
     gene            complement(2129..2503)
                     /gene="hisI"
                     /locus_tag="VF305_00310"
     CDS             complement(2129..2503)
                     /gene="hisI"
                     /locus_tag="VF305_00310"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX7415610.1"
                     /translation="MIEPNFAKGDGLVPTIVQNAETKEVLMMAYMNRQSWEATLQTGK
                     ATYWSRSRQTLWLKGETSGHFQLIKAIFIDCDNDTILLQVKQLGEAACHTGYRSCFYR
                     KLSGKDFVIVGEKIFNPKEVYK"
     gene            2704..2991
                     /locus_tag="VF305_00315"
     CDS             2704..2991
                     /locus_tag="VF305_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415611.1"
                     /translation="MSLLIGGAISAVFGLISLIFWWSDFITIIKGAFPIFFLLGGALA
                     IYVGIDEMQDKIKEERQRQDEKLEKAREEIELVKAQAERYKEELSKLKDIK"
     gene            complement(3230..>3758)
                     /locus_tag="VF305_00320"
     CDS             complement(3230..>3758)
                     /locus_tag="VF305_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX7415612.1"
                     /translation="GAREEGARVEGIHLSDFRITPCRECLSCFKDGVCVIADDMRGIY
                     SRLLEADIVILASPIFFYGITGWAKAMVDRCQALWARKYVLHDPTLGAEVEKRRGFFI
                     SVGGTKGKRMFEGAVLTVRYFFDAFNTDYTGELLFRGVDACGDIRKSPEALPQALTAG
                     RKLVSDLRGATLPRQ"
BASE COUNT         1068 a          881 c          697 g         1112 t
ORIGIN      
        1 ctgctccgtc agttggcgag cggtcatcca cttgccacca cctctaacga acgaagatat
       61 ttggaagaca tcttccatca gcgatttttg tgttttgtca aaaaatcccc gccaaattat
      121 gctgccatca atagttttaa tcaaatgaat ctcaaaagcc acagatgcag gatgttccga
      181 agagtagtcg taacccactc tttcggtata acggtaaata tatcccacgg ctaaaatatc
      241 cgcgtccaat tcattgccaa ctatttttaa gactttcagt aaaggttttt tcagcgattc
      301 ggaggcaatt cttttataga cgccttgcac cttatccggg ggtattattg ccacttcctt
      361 taattcgtgc agtttatcaa taaagacttc ctcgaccatt ttttcggatc cctttaatat
      421 ttttccactg gaataaccta tgccgcaaat tggacaaatt acagtacttc ccgattcttc
      481 ttcagggatt acgggctgaa aagaaattac ggcaatgcgc actggatgtt tagcttccac
      541 gcctttttcc gcagcttgag ctgtaatgga caaagtaagg agggtgacta aaacaaatcc
      601 tgcaatacaa ctaaattttt tccaaaaata attaagcaaa attattaccc ctctttttta
      661 aaggcgtcct ttggtagaaa gaactccatt aatattatca tttaaagtta cggcttttcc
      721 taaggctcgg gcaaaagcct tgaataccgc ctcaataatg tgatgactat tactgccata
      781 agccaggttg atgtgtaatg ttatgccgct atgatcggta aacgccttaa aaaattcttt
      841 aaccagggcc ggatcaaatc cgccgatttt cctgcgttca tatttaatgt tgtaaatcag
      901 ataggggcgg ccggaaatat ccaaatccac acggcataaa cattcatcca tgggcacgga
      961 ggccgaaccg taacgattaa tgcccatctt tttacccagc gcttgtccca tggcctgccc
     1021 cagacaaata cctatatctt ccaccaaatg atggtcatca atttgtgtgt cccctttgct
     1081 cttgataata agtttaaaaa aaccatgccg cgcaaaaagc tccagcatgt ggtcaaaaaa
     1141 gggaattgtc gtatcaatct tgcttcccgc agctttatcc aagtcaattt cgagttgtat
     1201 attggtttct gttgttttgc ggataatttt tactcttctg gccataaccg tcttcctgat
     1261 taaataactt tgtttaaagg atattcaatt ataccctgcg cccctgcctc taaaagcaca
     1321 ggaatcaaat cccgcataat tcttgcgtcg acaatggatt ctacagcaaa ccattcttcc
     1381 gaagacaaac tggaaattgt gggagctttg agtgaaggaa gtaatgtcat caccttaccc
     1441 aaattttctt tagaaacatt tagtttcaac cctactttac ccatcgcttg cagcgaacct
     1501 gtaagcattg ttttgatttg ttcaattttc ttacgcttcc aggaatcttg ccaggaagca
     1561 cgattggcaa tcaattgcgt gttggttttc attaattcgt gaataatccg taatttattg
     1621 gccttgattg tggaaccggt ttctgtgact tccacaatgg catccaccaa accttccacg
     1681 actttagctt ctgtcgcccc ccaggaaaat tcaacatgaa cgttgatatt tttctgggca
     1741 aagtattttt ttgtaaaatt aactaactcc gtcgatattt ttttcccttc catgtcgtca
     1801 attttttgat aaggcgaatc ctcgcggacg acaagcaccc agcgcgcctg ccgcatggaa
     1861 actttggaat aaatcaaatc ctgcacgaca acaacatccg acgaattttc caaaatccag
     1921 tcgatccccg ttagtcctaa atccaaagtt ccatcttcaa tataacgcga aatttcctgt
     1981 gcacgaacca gcgtacatga aatttccgga tcatcgacat cgggaaagta gctgcggctg
     2041 tcataggtaa tacgccagcc cgccttttta aataaatcca cggtattttc ttccagagac
     2101 cccttgggaa tccctaattt taaaactttc atttatatac ttccttagga ttaaaaatct
     2161 tttccccaac gataacaaaa tctttgccgc ttagcttccg gtaaaaacag gatctgtaac
     2221 ctgtatgaca agccgcttct cctaattgct ttacttgcaa taagattgta tcgttatcgc
     2281 aatcaataaa aatagctttg attaattgaa aatgtcctga tgtttctcct tttaaccaaa
     2341 gggtttgtcg tgacctgctc cagtaagtag ccttacccgt ttgcagagta gcctcccatg
     2401 attgacggtt catgtaagcc atcatgagaa cttctttagt ttcagcattt tgaacaattg
     2461 tggggactaa accatcccct ttggcaaaat ttggctcaat cattttatcc ttatatctca
     2521 ctatatctca ctaaagtagt attatttaaa tgatgaattg ctttaaatca agaaatattt
     2581 ttatcttgaa taatcatcta tttccggctt gcctaagctt ttttttcatg atagaaaaaa
     2641 acaagattgt attgtgattt actattgttt aattaatttg ttatttttac tggaggtata
     2701 tttatgtctt tgctcattgg aggagccatt tctgccgtct ttggattgat cagtttaata
     2761 ttttggtggt cagacttcat aaccataatt aaaggggcgt tcccgatttt tttccttttg
     2821 ggtggagctt tggctattta cgtgggaatc gatgaaatgc aggacaaaat taaagaagaa
     2881 agacaaagac aagatgaaaa attggaaaaa gccagggaag aaattgaatt ggtgaaggcc
     2941 caggctgaac gctataaaga agaattgagc aaattaaaag atataaaata gtaaagacta
     3001 ataatgaaac taagcccaat aaatgccgtt tgatgtgacc tgtgcaatga aatattagcc
     3061 tgtttcattt taaatccata gcacagtcat gtagaaaaaa ttgtttccga taggtacctt
     3121 gaaagcaatg ttttatccaa gccggccgcc atcagatagc atcgcatcct caccgcctgc
     3181 tctttgttct cttgctgaat caacatcata cccgtcaaac cccgtccctc tattgtcgtg
     3241 gaagggttgc gccacgcaga tcggaaacca gcttcctgcc ggcggttaag gcctgcggaa
     3301 gcgcctcggg cgatttccgt atgtcaccac aggcatcaac tccccggaag agcagttcac
     3361 ctgtataatc cgtattgaag gcatcgaaga aataccggac cgtcaggacg gccccctcga
     3421 acatgcgctt accctttgtc ccacccacgg aaataaagaa gcccctcctc ttctcgactt
     3481 ccgctcccag agtgggatca tggagcacgt attttcttgc ccacagcgcc tgacaacggt
     3541 caaccatggc cttggcccag ccggtaatcc cgtagaagaa tataggagag gcaaggatga
     3601 caatatccgc ctccaacaac cgggaataaa tgccccgcat gtcatcggcg ataacacaga
     3661 cgccgtcttt gaagcaactg aggcactccc ggcagggagt aattctgaaa tcactcagat
     3721 gtatcccctc cacccgcgcc ccctcctctc ttgccccc
//
LOCUS       DASVIM010000016         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_346347, whole genome shotgun sequence.
ACCESSION   DASVIM010000016 DASVIM010000000
VERSION     DASVIM010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346347"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VF305_00325"
     CDS             <1..560
                     /locus_tag="VF305_00325"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX7415613.1"
                     /translation="QGQLQELIILGELAKRACAAGVQVMIEGPGHVPITEIEANIKLQ
                     KKLCQGAPFYVLGPLPTDIAPGYDHITSAIGGAIAGAAGADFLCYVTPAEHLRLPTLA
                     DVREGVIAARIAAHIADIAKGVQGARERDRKMSECRKNFDWQGQVDLSIDPEKTVALL
                     EKSKSAQDEGCTMCGEFCAIRLGKR"
     gene            687..2036
                     /locus_tag="VF305_00330"
     CDS             687..2036
                     /locus_tag="VF305_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX7415614.1"
                     /translation="MSNNDEKLADDKRQHAEEIVRRLKKKGFEAYFVGGCVRDFVRGN
                     IPGDYDIVTSAHPDQVMAQFDHTVAIGAKFGVVAVIVENHPYEVATFRSDDVYEDGRR
                     PTQVHFSSAKEDVLRRDFTVNGLLMDPATGEIIDYVDGRADIEKKIIRAIGDPDARFN
                     EDYLRMLRAVRFAANLDFTIEQATQKAIERNAAKIKQISAERLQEELSKILTRGGARR
                     GFELMMQLGILREILPEIDKMQGVEQPPRFHPEGDVWQHTLMMLDMLPKDGENNANLC
                     LAWGTLLHDVGKVLTRSEDENGVHFYGHVQPGEEIADDIMQRLKFSRAQRETILNLIH
                     EHMVFMNVQKMRPGRLKRFLRMPDFDLHLELHRLDCLASHGMLDNYEFCRGQLQNLAV
                     EDLHPPRLLTGDDLIALGFTPGKIIGDILRALEDEQLEGRISTQEDAKKYVQKYWKK"
     gene            2116..2880
                     /locus_tag="VF305_00335"
     CDS             2116..2880
                     /locus_tag="VF305_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX7415615.1"
                     /translation="MKNNRKKLFILFIISLFVLSCASRTADVRPVEPVSQKKALELMG
                     YTIQAGAFAQVENAARLTETLKVRGLDSTYFKAADGLFKVRFGNFITKEKARQRAQNL
                     KEAGIIQEFYIVRPEDYAVAGQKRYGGNYLREALVKTAQDFIGVRYLWGGASSGKGFD
                     CSGLTMTVYQLNGLNLPRQSIKQFEVGSPVSSSKLQKGDLVFFALKGRNRVSHVGVYI
                     GDGQFIHASSRGKKIRVDSLSAASIANKYMGGRNYL"
BASE COUNT          913 a          606 c          770 g          835 t
ORIGIN      
        1 atcagggaca attacaggaa ttgatcattc tgggagaact ggcgaaaaga gcgtgtgccg
       61 ctggagtgca ggtgatgatt gaaggaccag ggcatgttcc cataactgag attgaagcca
      121 atattaaact gcaaaaaaaa ctttgtcagg gcgctccgtt ttatgtctta gggcctctgc
      181 cgactgatat tgctcctggt tacgatcaca ttacctccgc catcggcggg gcgattgccg
      241 gcgctgccgg ggcagatttt ttgtgctatg tcactccagc cgaacactta cgtctgccca
      301 ctctggctga tgttcgcgaa ggagttattg ccgcgcgcat tgcagcgcat attgctgata
      361 ttgccaaggg tgttcagggg gcgcgtgaaa gagaccggaa aatgtcagag tgccggaaaa
      421 actttgattg gcaggggcag gttgatttat ctattgatcc ggaaaaaaca gttgcgctct
      481 tagaaaagag caaaagcgca caggatgaag gctgcaccat gtgcggcgaa ttttgcgcaa
      541 ttagattagg caaaagataa ttaatgcccc ttcgcttgaa atctaaatgg tattataaaa
      601 aagaggatgg agaattggaa taagaccagt gcaataggga tagcggtgtg tagatggcca
      661 tgcaataata tgttgaggat taatccttgt ctaataatga tgaaaaatta gctgatgata
      721 aacgccagca tgcggaagaa attgtccgcc gactgaaaaa aaaaggcttt gaagcatatt
      781 ttgtcggcgg ttgcgtgcgg gactttgtgc gcggtaatat tcccggcgac tacgatattg
      841 taacttcggc acatcctgat caagtaatgg cgcaatttga ccacaccgtg gccattggcg
      901 caaagttcgg agttgttgcc gtcattgtgg aaaaccatcc ttacgaagtg gcaacatttc
      961 gcagtgatga tgtctatgaa gacggcaggc gtcctactca agttcatttt tcttcggcta
     1021 aagaagatgt cttacggcgc gattttacag ttaatggttt gttgatggac ccggcaaccg
     1081 gtgaaataat cgattatgtc gatggccgtg ccgatattga aaaaaaaata atccgcgcaa
     1141 ttggtgatcc cgacgcccgt tttaatgaag actatctgcg gatgctccga gccgtacgtt
     1201 ttgccgctaa cttggatttt acaatagagc aggccacgca aaaagcgata gaaagaaatg
     1261 ccgcaaaaat taaacaaatc agcgcggaaa gattgcagga agaactgagt aaaattctca
     1321 cgcgtggtgg cgcccgccgc ggcttcgaat taatgatgca actaggaatt ttacgggaaa
     1381 ttttaccgga aatagataag atgcagggag tggaacagcc gccgcgtttt catcctgaag
     1441 gtgatgtctg gcaacatact ttaatgatgc tggatatgtt gccaaaagat ggagagaaca
     1501 atgcaaatct atgtctggcg tggggaacac ttttacatga tgtcggcaaa gttctcacgc
     1561 gcagtgaaga cgaaaacggc gtgcacttct acgggcatgt tcaacctgga gaagaaattg
     1621 ccgatgatat aatgcagcgc ctgaaatttt cccgagcgca aagggaaacg attctcaatc
     1681 taatccacga gcatatggtt ttcatgaatg tacaaaaaat gcgcccggga cgactcaaaa
     1741 gatttttgcg catgccggat tttgatttgc atctggagtt gcatcgtctt gattgcctgg
     1801 ccagccacgg catgctggac aattatgaat tttgccgcgg tcaactgcaa aacttagctg
     1861 tggaagattt gcacccgcca cgcttactca ccggtgatga tttgattgct ctgggcttta
     1921 ctccgggaaa aattatcggg gacatcctgc gagctttaga agatgaacaa ttggaaggca
     1981 ggatctctac acaggaagac gcaaaaaaat acgttcaaaa atactggaaa aaataaattt
     2041 gcacctgcct tttttcctgg taaactatca tttagaagat caatctttaa aaaatattca
     2101 aagaatgatg aatcgatgaa aaataaccgg aaaaaattat ttattctttt tataatatct
     2161 ttgtttgttt tatcttgtgc cagccgaacc gcagatgtaa gaccggtcga accggtgtca
     2221 caaaaaaaag cattggagct gatgggctat accattcagg ccggtgcatt tgctcaagtg
     2281 gagaacgcgg cgcgtttaac ggaaacactt aaagtacgag gattggattc cacatatttc
     2341 aaagctgctg atggattatt taaggttcgg ttcggtaatt ttattaccaa ggaaaaggcc
     2401 agacaaagag ctcagaatct taaagaagca ggaatcattc aagagtttta cattgtacgg
     2461 ccggaagact acgctgttgc cggacaaaag cgatacggtg gcaattacct gcgagaagct
     2521 ttggttaaaa ctgcccagga ttttatcgga gtccgttatc tttggggcgg cgcttcttcc
     2581 ggtaagggat ttgattgtag tggattgaca atgactgtgt atcaattaaa tggcctgaat
     2641 ttaccccgcc aatcaataaa acagtttgag gttggtagtc cggtgagtag cagcaagttg
     2701 caaaaaggtg atttagtgtt ttttgctttg aaaggaagaa acagagtttc gcatgtggga
     2761 gtttatatcg gagatggaca atttatccac gcttcctcac ggggcaaaaa aattcgcgtc
     2821 gattccctgt ccgctgcttc cattgccaat aaatatatgg gtggaagaaa ttatctctaa
     2881 ttgataagag tgttaaatta tttgtgataa aaatttgaat tcggtagcga gcgcattccc
     2941 cgtagcttgc tgcggagagc ttcaattaat tttgcgcaaa gttcgaagta aaagtaattt
     3001 tggaaaaata tttttcattt atcagttccc acttctgctt taagagagtc aacccaatta
     3061 atctagctcg gcagattcta ctgccccaaa aatcattttc gtgcttcata gatttctttg
     3121 ttga
//
LOCUS       DASVIM010000017         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_516337, whole genome shotgun sequence.
ACCESSION   DASVIM010000017 DASVIM010000000
VERSION     DASVIM010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516337"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VF305_00340"
     CDS             <1..1191
                     /locus_tag="VF305_00340"
                     /inference="COORDINATES: protein motif:HMM:NF027594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HEX7415616.1"
                     /translation="PNVSYGEDYAVALRISREYQIGRIYKSIYLCRRWRGNTDAALSI
                     EKQNRNDFYKDELRTMEIKARQILNKKKKDLEKRIFAQYPVKKQKSLTTLCLNLLEEQ
                     KKSWPGLAYAYQELANVGIRSISYGSYKVDLQFNPQRAVSSGAAVDRESIKNRPCFLC
                     TDNLPIKQKGILYQNNYLILCNPAPIFDKHFTIAHLRHQPQTIADSLIGLMNLAKDLA
                     PDFTVFYNGPACGASAPDHLHLQAIPKNALPFIEASKTQSPIKEISGVKFYTGEKLDR
                     AVVILTGTRKDSLQKQFGHLIKAAQKIIPTDSEPMLNVLCTYENTTWRLIIFLRQKHR
                     PDAFFLEGEKRIFISLGAIDMAGIIITPMLADFNRLKAFQISSIYREVSLAEDVTNKI
                     IREL"
     gene            1205..1759
                     /locus_tag="VF305_00345"
     CDS             1205..1759
                     /locus_tag="VF305_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX7415617.1"
                     /translation="MSDDDNVIVDSDFSFSDAIAGTNAPLEIVDRLSMLDVYYYSFDG
                     MRHQGQIIVDCSLEDDVYEIFSLIGKLKFPIGKAIPIVAYSWQDGESMAANNTSCFNF
                     RVIEGTTKLSLHSFGRALDINPVQNPVIYPHGLIAPEGAVYNPQKRGAFTAANPIVQE
                     FLKRGWHWGGNFEQPKDYHHFEKP"
BASE COUNT          584 a          442 c          373 g          515 t
ORIGIN      
        1 cccaatgtca gctatggtga agattacgcc gtcgccctgc gaatatcgcg cgaatatcaa
       61 atcggacgaa tttataagag catttatctt tgccgccgct ggcggggaaa taccgatgcc
      121 gctctctcca tcgaaaaaca aaaccgtaat gatttttata aagacgaact acgcacaatg
      181 gaaataaaag cacggcagat actaaacaaa aagaaaaaag atctggaaaa aagaatcttt
      241 gcccaatacc ccgtaaaaaa acaaaagtcc ctgactactc tgtgcctgaa tctattagag
      301 gaacaaaaga aaagctggcc tggacttgct tatgcttatc aagaattggc aaatgttggc
      361 atacgctcaa tttcttacgg cagctacaaa gttgatctgc aattcaatcc tcagcgcgct
      421 gtcagcagcg gcgccgcagt tgaccgggaa tcaattaaaa acagaccttg ctttttgtgc
      481 actgataatc ttccaatcaa gcagaaagga atcctttatc agaacaatta tctcatcctt
      541 tgcaatccgg cacctatttt cgataagcat tttactattg cccatttaag gcatcaaccg
      601 caaacaattg ccgattcatt aatcggcctg atgaacttag ctaaagattt agctccagat
      661 tttactgtgt tttacaacgg ccctgcctgt ggagcatcgg ctcccgatca tttacattta
      721 caagcaattc ccaaaaacgc actgcctttt atagaagcat ccaagactca atctccgatt
      781 aaagaaatat ccggcgtaaa attttacacc ggagaaaaac tcgaccgcgc cgtagttatt
      841 cttacaggaa caagaaaaga ctctctgcaa aaacaattcg gtcacttaat taaggccgcg
      901 caaaaaatca ttcccacaga cagcgaacca atgctcaatg ttctttgcac ctatgaaaac
      961 accacctggc ggctgataat atttttgcgg cagaaacacc gtccggacgc cttctttctt
     1021 gagggcgaaa aaagaatttt tatctcttta ggcgcgatag atatggccgg aataatcatc
     1081 acaccaatgc ttgcggattt caatcgtttg aaagccttcc aaatcagcag tatctaccgg
     1141 gaagtgtcgc ttgcggaaga tgtaacaaat aaaataattc gggaattata gcggataatt
     1201 aattatgagc gacgatgata atgtaattgt ggatagcgat tttagttttt ccgatgctat
     1261 tgcgggaaca aacgcacccc tggaaatagt cgatcgctta tcgatgttag atgtctacta
     1321 ttactctttt gacggcatgc ggcatcaggg acaaattatt gttgattgct ctctggaaga
     1381 tgatgtttat gaaatttttt ctctaattgg aaaattaaag tttcccatcg gcaaagctat
     1441 tcccattgta gcctatagct ggcaagatgg tgaatccatg gctgccaata acacatcctg
     1501 ctttaatttt cgcgtaatcg aaggcacaac aaaattatcg ctccactctt tcggccgtgc
     1561 cctggatatc aatccggtgc aaaatcccgt aatctatccc catggcctta ttgctcccga
     1621 aggtgcagtt tataacccgc aaaaaagggg agcttttact gcggcaaatc ctatcgtgca
     1681 ggaattttta aaacgcggct ggcactgggg cggtaatttc gagcagccga aggactatca
     1741 tcattttgaa aaaccctgat accaattcca aatcaaagcg ctaccggcgt tggcttcttc
     1801 atcggctcct cgacgtataa taacatacgc ctgtgtcgcc tcttcctcgc cgccttgata
     1861 tcatcttaga tttgaaattg ctataataac aattttctta attatagagg acgg
//
LOCUS       DASVIM010000018         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_431754, whole genome shotgun sequence.
ACCESSION   DASVIM010000018 DASVIM010000000
VERSION     DASVIM010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431754"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VF305_00350"
     CDS             <1..737
                     /locus_tag="VF305_00350"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7415618.1"
                     /translation="TYEEKGDNKAAVDEYRKIPDTSELYINAQIHAAMILKKEGKSTE
                     AINLIKEAAKKKNDKVPFYLYLSSLYEDAKDTATAENILNEGIKIASQDADLHYALGV
                     IYEKTNRFPESIKEMEMVLRLDPDNAEALNFIGYSYADRGMNLEEAEKMIIKALKIKP
                     DNGYIIDSLGWVHFKQNKMDSAVKHLKQALELLPDDVNITEHLGDVYVKIGKFKEAQE
                     IYQKALKIDPKNTAVQQKLEDLIKKK"
     gene            787..1569
                     /locus_tag="VF305_00355"
     CDS             787..1569
                     /locus_tag="VF305_00355"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HEX7415619.1"
                     /translation="MHNVYFRLLKLIIILLSALLIGGCLQREVIKPDSRVEKVLMAVS
                     DAIAENDVLSAMAQIDLVTPHGYNTVRAALIIKKPSYLRLELLPIIGTPDFFLSSSPA
                     EMCIFIPSRGEFYRGKPTAANLASFLPWQFNIEDIVMIFTGAYPPIIDGIISYQSYQE
                     NNSLRIEMKSPSDKSQILWVGENNRLLKLASLNEFGQEIYHVNYEDYRPESPVPGKIT
                     INMADGITSISVRYTEIKIEKAADLSVFELPVPAGVKLILLD"
     gene            complement(1639..>2123)
                     /gene="ybgF"
                     /locus_tag="VF305_00360"
     CDS             complement(1639..>2123)
                     /gene="ybgF"
                     /locus_tag="VF305_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX7415620.1"
                     /translation="FIENFLEIGKKDTLSDTPDKNSKSTGNSVAKDPAKKQDKEKAYS
                     AAYQTFKEGNYDKAMTEFQKFLTDYPDSEYSDNAQFWIGECYFFKKNYEQAILEYEKV
                     VKNYSSGNKVPYALLKQGLSFLNLRDKTSAKLLLQQVIKDYPNTNQARIARSKLQEIK
                     "
BASE COUNT          662 a          385 c          420 g          656 t
ORIGIN      
        1 ctacttatga agaaaaaggc gacaacaaag cggcagtgga cgaatatcga aaaattcctg
       61 atacatccga attatatatt aacgctcaaa ttcacgccgc catgatttta aaaaaagaag
      121 gtaaaagcac tgaggccatt aatttaataa aggaagccgc taaaaaaaag aacgacaagg
      181 ttccttttta cctttatttg tcttcacttt atgaagacgc caaagatact gctacggcag
      241 aaaacatctt gaatgaggga atcaaaattg cttctcagga tgctgatttg cactatgcgt
      301 tgggcgtgat ttatgaaaaa acaaaccgct ttcccgaaag cataaaagaa atggagatgg
      361 ttttgcgtct tgatccggat aacgccgagg cgcttaattt tataggctac agttatgctg
      421 atcgcggcat gaatctagaa gaagcggaaa aaatgatcat caaagctttg aaaattaaac
      481 ccgataacgg ttacataata gatagtcttg gctgggtgca ttttaaacaa aataaaatgg
      541 atagcgccgt aaaacattta aaacaagctt tggagctttt gcctgacgat gtcaatatta
      601 ctgagcatct gggcgatgtt tacgtaaaaa tcggaaaatt taaggaagct caggaaattt
      661 atcaaaaagc gctgaagatt gatcctaaaa acacagcggt acaacagaag ctcgaagatt
      721 taattaagaa gaaataacaa ggaaaatttc ttatccacat aatattttcc ggaaaatatt
      781 tgctccatgc acaatgtata tttccgtttg ctaaaattaa ttattatttt attgtcggcg
      841 ttgcttattg gcggctgcct tcaacgggaa gtcattaaac ccgattctag agtagaaaaa
      901 gttctgatgg ctgtttccga cgctattgcc gaaaatgacg tattgagcgc tatggcgcaa
      961 attgatttgg ttacacctca tggttataat actgtaaggg cagccctaat tattaaaaag
     1021 ccgtcatatc tccggctgga actgctgccg attatcggaa cgccggattt ttttcttagc
     1081 tcttcaccag cagaaatgtg tatctttatt ccttccaggg gggaatttta tcgcggcaag
     1141 ccaaccgccg caaatcttgc gagtttcttg ccgtggcaat ttaatattga agatatagtc
     1201 atgattttta ccggcgctta tcctcctatc atagatggga ttatatccta tcaaagctat
     1261 caagaaaata attctttgcg tatagaaatg aaatcgccat cagataagtc gcaaatatta
     1321 tgggtggggg agaataatcg tttgttgaaa cttgctagtt taaatgaatt tggccaagaa
     1381 atatatcatg tgaattacga agactatagg ccggaaagcc ccgttcccgg gaaaattacc
     1441 atcaacatgg ctgatggcat aacttccatc tccgtaagat atacggaaat caaaatagaa
     1501 aaagctgctg atttgtcggt ttttgagttg ccggttccgg ctggtgtgaa actaatttta
     1561 ttagattagg tttataatat aaaaaaaggg ggtttaaatt cccccctttt ttttgttttt
     1621 ttatccgcaa taaattaatt actttatttc ctgcagtttc gaacgcgcta tgcgtgcctg
     1681 attggtattc ggataatctt taattacctg ctgcaggagc aatttagcgc ttgttttgtc
     1741 tctcaaattt aggaaggaga gaccttgctt taataaagca taaggaactt tattgccgct
     1801 cgagtaattc ttaactactt tttcgtattc caaaattgcc tgttcataat tttttttaaa
     1861 aaagtaacat tcaccgatcc aaaactgggc attatcggaa tactcgctat caggatagtc
     1921 agttaaaaat ttttgaaatt cggtcatagc tttatcataa ttgccttctt tgaaggtctg
     1981 gtaagcggcc gaatacgcct tttccttatc ttgctttttt gccggatctt tggccacaga
     2041 gttaccggtt gatttactat tcttatctgg ggtatcgctt aaagtatcct tcttgccgat
     2101 ttccaaaaag ttttcgatga aat
//
LOCUS       DASVIM010000019         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_347199, whole genome shotgun sequence.
ACCESSION   DASVIM010000019 DASVIM010000000
VERSION     DASVIM010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347199"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VF305_00365"
     CDS             complement(<1..190)
                     /locus_tag="VF305_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415621.1"
                     /translation="MGFSRYRYLIFGILGAAYILVFFHRLAPAIVAVDMMRDLQTSGA
                     LMGILASAYFYPYALMQIP"
     gene            complement(294..644)
                     /locus_tag="VF305_00370"
     CDS             complement(294..644)
                     /locus_tag="VF305_00370"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7415622.1"
                     /translation="MKIINYTSVTPTNFDNDQAKGVAGRVVVGKKDGANNFCMRIFEI
                     APGGNTPKHAHAWEHEMFIHSGVGEIYGNGKWNPVQPGTVIFMPPNEEHQVKNTSSKP
                     LILACLIPSIAPEL"
     gene            complement(1303..>1623)
                     /locus_tag="VF305_00375"
     CDS             complement(1303..>1623)
                     /locus_tag="VF305_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX7415623.1"
                     /translation="SKIKNPKDFEVLVNPRITQARGEKVTMAEGCLSCPEIQVEVSRF
                     PEIKVRALNAQGEKIGKRYLDYVARVIQHELDHLDGKLIVDYGGNLYYPKDKQDFFEK
                     IFKG"
BASE COUNT          481 a          342 c          326 g          474 t
ORIGIN      
        1 cggggatttg cattaaggca taggggtaaa aataggctga agccagaatg cccatcaacg
       61 ccccgctggt ctgtaaatca cgcatcatgt cgaccgccac aattgcgggg gcaagcctgt
      121 ggaaaaaaac caaaatataa gcggcaccca aaatgccgaa tatcagatac cgatacctag
      181 aaaatcccat atcaatcttc ttggagaagt aatgttttcc ttccagttaa taaataatcc
      241 aatcagctag cagaggcaag aatattttgc tccaagctta gtgcaaccca gcattataat
      301 tccggggcta ttgaagggat gagacacgcc agaatcaatg gtttactgct ggtattttta
      361 acttgatgct cttcattagg cggcataaaa ataacagtgc cgggctggac gggattccac
      421 ttaccattgc cgtaaatttc accaacacct gaatgaataa acatttcatg ttcccaggca
      481 tgggcatgct tcggtgtatt tccacccggc gcaatttcga atatgcgcat acaaaagtta
      541 ttcgcaccat ctttcttacc gacgacaacg cgaccagcaa ctccctttgc ctggtcatta
      601 tcaaaattgg ttggtgtaac ggatgtgtaa tttattatct tcatgatgtt ctccttttta
      661 actattctat ccttatgggg aatatgaact ttaacttata gcagcggaga ataaatttca
      721 aagccatctt caccggcgta accgctgcgg gaagtgatgg acggtattcc cgatacatga
      781 gattcgaaaa aatgatagaa gcgcaaattc ttcaaattat caaaggtcag tgtttgcaaa
      841 atttcttcag ctaaccaagt atttaaaaat tagagataga ataatgagaa tcaagcaaac
      901 cgccaaaaat aaataactct tttggaaagc tttactgccc agcacaagct tcccgcaatg
      961 ctggcaaata gtaaatgtgg atgtaatttt tccctggcaa aaggggcaac gcagagtttt
     1021 tggctccacc ttggtttcac gccggataat atttctcttt agttcgttat gaatattata
     1081 atgccagtta caatccagcg tagggcattt gttctgcgct ggcatattgt ctgtccaaac
     1141 aaaagatttg ttacattgcg ggcactgaat tttcaacatg ggatagcttt tccgggggaa
     1201 attaagttat agaaaataac tttattgtat cagttttaat taatgccaga gaactactac
     1261 aactccggga agatttcaat atttaatcaa attttccgcc ggtcaacctt taaaaatttt
     1321 ttcaaaaaag tcttgtttgt ctttgggata atagagattg cctccgtaat cgacgattaa
     1381 tttaccgtcc agatgatcca actcgtgttg tatgacgcgg gcgacataat ctagatatct
     1441 tttaccgatt ttctctcctt gggcatttaa ggcgcgcact ttaatttcgg ggaaacggct
     1501 gacctcaacc tgaatttccg ggcaagataa acagccttca gccatggtta ctttttcgcc
     1561 gcgcgcctga gtaatgcgcg gattgaccag aacttcgaaa tctttgggat tttttatttt
     1621 gct
//
LOCUS       DASVIM010000020         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_860785, whole genome shotgun sequence.
ACCESSION   DASVIM010000020 DASVIM010000000
VERSION     DASVIM010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860785"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1005
                     /locus_tag="VF305_00380"
     CDS             163..1005
                     /locus_tag="VF305_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX7415624.1"
                     /translation="MDYLYKVKMAKDIFIGEILNNEEIQGNCFLMKVILPASFCDPQP
                     GQFVMIRISGLYEPLLARPISIYSFSRGKNSCAIELLYSVVGKGTQILAGLISGSQVE
                     IHGPLGRGFEIDEARQNIIFIAGGMGIAPLSLLTEHLCGDKCFSKSAMIFYLGAKTAK
                     AVVGLDKLQKLCYNINVCTDDGTLGKKGLVTQAFQKDIKKFAPANTVIYACGPKEMLR
                     SLAKMLRGSKFSSQVSLEERMACGIGACLGCAVAIRNDKGALAYKRVCADGPVFDIQN
                     IIWK"
     gene            1008..>1535
                     /locus_tag="VF305_00385"
     CDS             1008..>1535
                     /locus_tag="VF305_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX7415625.1"
                     /translation="MDKIKTKTSVQMAVNLGRLKLKNPVMTASGTFGYGEEYADYVDL
                     NRLGAVVVKGLSLKPRLGNPPPRIMETASGMLNAVGLQNIGVDAFIEEKLPLLQKFDV
                     KVIANIYGETYDEYKKVAKKLSMAKGVHALEVNISCPNVKKGGVSFGADPKITAMVTR
                     KVKDETSLPVIVKLTP"
BASE COUNT          454 a          289 c          386 g          406 t
ORIGIN      
        1 caataaaact ttacgagaaa tgcggttttg tagtaaaagg aagaagacca ctttattacc
       61 cggaaacaca tgaagatgcc ttgattatgt gggcggatgt agcaaaatga tttttatagt
      121 gtcattgcga ggagcgcctc gcgcgacgcg gcaatctatt taatggatta tctctataag
      181 gttaaaatgg ccaaagatat tttcatcgga gaaattctaa ataacgaaga gattcaaggg
      241 aactgctttt tgatgaaagt aatcctgccc gcctcgtttt gtgatccgca accggggcag
      301 tttgtgatga ttagaatttc aggcttatat gaaccgctct tggcacggcc gataagtata
      361 tattcttttt ctcgcggcaa gaattcctgc gcgattgaac ttctctactc tgtagtgggc
      421 aagggaacgc aaattctggc gggattgatc agtgggtcac aagtcgaaat ccacggacca
      481 ttgggtcgcg gctttgaaat cgatgaggct agacaaaaca tcatttttat cgccggagga
      541 atgggcatag ctccgctgtc actgctgacc gagcatttat gcggggataa gtgtttttct
      601 aagtccgcga tgatttttta cctgggcgct aaaaccgcta aggctgttgt aggtttggat
      661 aaactgcaaa aactttgtta caatattaac gtttgcaccg acgacggaac tctggggaaa
      721 aaaggattag tgacacaagc atttcaaaaa gacataaaaa aatttgcacc ggcaaacact
      781 gtaatttatg cctgcgggcc aaaagaaatg ctcaggtcac tggcaaaaat gttaagagga
      841 agtaaattca gctctcaggt gtcgctggaa gagcggatgg cctgcggcat tggagcttgc
      901 ctgggttgtg cagtagccat taggaatgat aaaggtgccc tggcctataa gcgggtttgt
      961 gctgatggtc cggtatttga tatacaaaat attatctgga aataattttg gataaaataa
     1021 aaacaaaaac atctgtacaa atggccgtaa atttagggcg gctaaagcta aagaatccgg
     1081 tgatgacggc gtccggcacc tttggttacg gagaagaata cgctgattat gttgacttaa
     1141 accggctggg agctgttgtc gttaaaggtc tgtcgctaaa gcctcgtttg ggaaatccac
     1201 cgccgcgaat tatggaaacg gccagcggta tgctcaatgc cgtcggtttg cagaatatcg
     1261 gcgtggatgc tttcattgag gaaaaacttc ccctcttgca aaaatttgat gtaaaagtca
     1321 tcgccaatat ttacggtgag acttatgatg aatataaaaa agttgcaaaa aagttaagca
     1381 tggccaaagg cgtacatgct ctggaagtaa atatttcctg tcctaacgtg aaaaaggggg
     1441 gggtaagttt tggtgctgat ccgaaaatta ctgctatggt gacgcgcaaa gtcaaagatg
     1501 aaaccagcct gccggttata gtcaagctta ccccc
//
LOCUS       DASVIM010000021         4760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_861327, whole genome shotgun sequence.
ACCESSION   DASVIM010000021 DASVIM010000000
VERSION     DASVIM010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861327"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /locus_tag="VF305_00390"
     CDS             <1..1823
                     /locus_tag="VF305_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7415626.1"
                     /translation="IGDALNRSLEVAQVYYQQMGEQAKFNARQISTDITKNRLYERER
                     VEYLNSFIGQRQKNYKVGMVETYFDFQKENIVFTDVEHPELVPVTLAPKMIEDIYSGK
                     EVTTVQPTNIGEAIVGIVPVFSYAVPAEVIGRISVSYSIPKGFVDKLRSIADASEQYG
                     QIKLLKNPIKFNYIITLSIVTLVIIFLATWFGLALAKGITVPIQNLAQATNRITQGDL
                     DSQIDIEADDEIGQLVKSFNQMTRDLKKSKHGLEEANISLEQRRKYIETILRNVSAGI
                     VSVDKTGMITTVNRAAEKILDIKPEKVLFQNYRDLMIPEHLSLVDEFLQEMKENNESL
                     LEKQLELMLKDKALTVLLTITTIQDEEGNDSGIVIVFEDLTQLQKAERAAAWREVARR
                     MAHEIKNPLTPVQLSAQRLQRKYGDKLGEEGSVFKECTQTIIDQVEVLKNLVNEFSRY
                     ARMPVTALTLNDLNEVVADSVLLFQDAHKDIVFEFSPAEGLPKFDLDAEQINRVMINL
                     LDNAVAAINKNDGRIEITVSYDEQHRKATVSVADNGAGVPSSYKRKVFEPYFSTKKSG
                     TGLGLAIVSSIISDHHGQVSVADNNPTGTNVSFQLPVSEV"
     gene            1870..3240
                     /locus_tag="VF305_00395"
     CDS             1870..3240
                     /locus_tag="VF305_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7415627.1"
                     /translation="MNETILVVDDEVSICQSLKAILSDEGYQVLVAGSGEEAIKIVEE
                     EMPLLVLLDIWLPGLDGLETLKAIKAAHPKVLVIMMSGHGTIETAVKATKLGAFDFIE
                     KPLSLDKVIILVNNALNLVRLEEENILLKQKVSHQYELTGNSAVITELKEMISIVAPT
                     NAWILIMGENGTGKELVARSIHHLSWRSHKDIVEVNCAAIPEELIESELFGHEKGAFT
                     GATEKKRGKFDLAHEGTIFLDEVADMSLKAQAKILRILQEKQFERVGGNKLIDMDVRV
                     LAATNKELEKEMEVGRFRQDLYYRLHVIPLRVPPLRERKEDIKLLAERFLLDFAVKEG
                     QAPKILTADALASLMEHDWPGNVRELKNIIERLIILTPSNEIKAADIPLLNVKGEQTA
                     TVIESFIANDSLKDAKMDFERQFILKKLEENEGNISKTAEAIGLERSNLHKKLKILKI
                     AAKDEL"
     gene            complement(3257..4255)
                     /locus_tag="VF305_00400"
     CDS             complement(3257..4255)
                     /locus_tag="VF305_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX7415628.1"
                     /translation="MNKKIIFGIVLSVILIYLSVRGINFQDTLHDLKKIQLSYVAFFI
                     GIVFLMQYLRSYRWGIILQPLQKIDQFSLFSVTSVGFLAIAAIPARLGELARPYLIAK
                     RSTIKMSSALGTILVERVLDGFSILTIAVVVLFYTDLPSWMIKSGILFALLTILMFFC
                     IAGLIWHRETAIKIINRILSKLPGKFAHKVDELIHHFIDGFQVIKNVKMLFYLFFLSA
                     LIWLVDVLSIYVLFLAFGFTLPVMASFVVMIVLIIGIAIPTAPGFIGNWHFACILGLS
                     IFGIAKPEAFSFAVVYHFLSMAFTLAFGVIFLPFNKFSLADMKKQMTQKKISEEMK"
     gene            complement(4295..4696)
                     /locus_tag="VF305_00405"
     CDS             complement(4295..4696)
                     /locus_tag="VF305_00405"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 domain-containing protein"
                     /protein_id="HEX7415629.1"
                     /translation="MKRNIFCLLISLFALFIFSSCAALVVGGAAVGASTGTYFYVDGE
                     LKNDYFAPFDKVWSSCEKTVADMRGIEVAPVKEIARGRITTVINDEKVKFDITYRSKN
                     QTTVAIRVGLMGNKLSSQLLHDKIAENLAKN"
BASE COUNT         1520 a          891 c         1072 g         1277 t
ORIGIN      
        1 agattggcga tgccttaaac cggtcattgg aggtggctca agtttattac cagcaaatgg
       61 gggaacaagc caagttcaat gcccgtcaaa tcagcacgga tatcaccaaa aaccgtctct
      121 atgaacgtga aagagtggaa tatctaaata gtttcatcgg ccaacgccaa aagaattata
      181 aagtgggcat ggtcgagact tattttgatt tccaaaaaga aaatattgtt tttactgatg
      241 tcgaacatcc agagctggtt ccggtaactc tcgctcccaa aatgatagaa gatatttatt
      301 ccggcaaaga agtaactaca gtccagccaa ccaatatcgg agaggcaatt gttggtattg
      361 tgccggtctt ttcttatgcg gtgcccgcgg aagtaattgg ccgcatttcg gtaagttaca
      421 gtatccctaa aggctttgtg gataaattga ggagtattgc tgatgcctcg gaacagtatg
      481 gtcagattaa acttctgaaa aatcctatta aattcaatta tattattact ttgtccatcg
      541 tgaccctggt aatcatattt ttagcaacgt ggtttggact tgcgctggcc aaaggaatta
      601 ctgtccctat tcaaaatctt gctcaagcca caaatagaat tacgcaggga gacttggata
      661 gccaaattga tattgaagcg gacgatgaaa ttggacaatt agtcaagtcc tttaatcaga
      721 tgaccaggga tttaaagaaa agcaaacatg ggttggaaga agctaacatt agtttagaac
      781 aacggcgcaa atatatcgaa acaattttgc gcaatgtttc ggcaggtatc gtttccgtgg
      841 ataaaaccgg gatgatcact actgttaatc gcgccgcaga gaagatatta gacattaaac
      901 cggagaaggt tctttttcag aattatcgcg atctaatgat accggagcat ctttctcttg
      961 ttgatgaatt tttacaggaa atgaaagaga acaatgaaag cttattggaa aaacaattgg
     1021 aacttatgct taaagataaa gctcttacag tgcttctgac gattacaaca attcaggatg
     1081 aggaaggcaa cgattccggt atcgtcatag tttttgaaga tttgactcaa ttacaaaagg
     1141 cagaacgtgc tgctgcctgg cgtgaggttg cccgtcgtat ggcgcatgag atcaaaaatc
     1201 cgttgacgcc ggtgcagctt tccgcgcaaa gattgcagcg aaaatatgga gataaattag
     1261 gagaagaagg ttccgtattt aaagaatgta ctcagacaat tattgatcag gtagaggtgc
     1321 tgaaaaatct ggtcaatgaa ttttcccggt atgcacgtat gcctgtgact gctctgacac
     1381 tcaatgatct caatgaggta gttgccgatt ctgttttgct ctttcaagat gcccataagg
     1441 atatcgtctt tgaatttagt cctgctgaag gattaccgaa gtttgatctt gatgccgagc
     1501 agatcaacag ggtaatgatt aatttgctcg ataatgccgt tgcggccatt aacaaaaatg
     1561 acggccgcat cgaaattacc gtaagttatg atgaacaaca ccgcaaagcc actgtaagtg
     1621 tggccgataa tggggccggg gttccctcaa gttataagag gaaagttttt gagccgtatt
     1681 tttctaccaa gaaatccgga acaggtttgg ggttggcgat agtaagttcg ataatttctg
     1741 atcatcatgg tcaggtaagc gttgccgaca ataatcccac aggaacaaat gtttcttttc
     1801 agttgccggt ttccgaagtt taaattatat gttgaaagaa aaataagcgg atgaactgaa
     1861 ataataatta tgaacgaaac aattcttgta gtcgatgatg aagtgagcat ttgccagtcg
     1921 ttaaaggcaa tcttaagcga tgaaggctat caggtgctag tggcgggcag cggcgaagaa
     1981 gccattaaaa ttgttgagga agaaatgccg ctgcttgtgc tgctcgatat ttggctgccg
     2041 ggcttagatg gtttggagac gctcaaagcc attaaagcag cacatccaaa agtgctggta
     2101 attatgatgt ccggccacgg cacaattgaa acagcagtga aggctacgaa actcggagcc
     2161 tttgatttta ttgaaaagcc tctttccctc gataaagtta taatcttggt aaacaacgcc
     2221 cttaatttag ttcgtctgga agaagaaaac atcttattaa agcaaaaggt ttctcatcaa
     2281 tacgaactga ccggcaatag cgcggtgata accgaactta aagaaatgat cagcattgtt
     2341 gcgccgacta atgcctggat tttgattatg ggggaaaacg gtacgggcaa agaattagtg
     2401 gcgcgttcta tccatcactt aagctggaga tcgcataaag atattgtgga agtcaactgt
     2461 gcggcgatac ctgaagagct tattgaaagt gaattatttg gccacgaaaa aggagctttt
     2521 accggagcga cggagaagaa acgcggcaaa ttcgatcttg cccacgaagg cacgattttt
     2581 ctagatgaag tggccgatat gagcctaaaa gcgcaagcaa agattctgcg cattctgcag
     2641 gaaaagcagt ttgaacgggt cggtggcaat aaattaattg atatggatgt gcgggtttta
     2701 gctgcaacta ataaggaatt ggaaaaggaa atggaggtgg gacgttttcg gcaggatctt
     2761 tattatcgct tgcatgtaat ccctttgcgg gttccgcccc tgcgtgaaag aaaggaagac
     2821 attaaacttt tggcggagcg tttcttgctg gattttgccg ttaaagaagg ccaagcgcca
     2881 aagatattaa ctgcagatgc attggcctcg ctgatggaac acgattggcc gggaaatgtc
     2941 cgggaattga aaaatattat tgaaagatta attatcttaa ctccatccaa tgaaataaaa
     3001 gctgcagata ttccattgct caatgtcaaa ggagagcaaa cggccacagt aattgaatcc
     3061 tttattgcca atgattcttt aaaagatgcc aagatggatt tcgaaaggca gtttatttta
     3121 aagaaactag aggaaaatga aggtaatatt tccaaaacag cggaagcaat tggcctggag
     3181 cgcagcaatc tgcataagaa attaaaaata cttaaaatag cggcaaaaga cgaattgtga
     3241 ttattgccat tactttttat ttcatctctt cgctaatctt tttttgagtc atctgttttt
     3301 tcatgtcggc aagagaaaat ttattgaagg gaagaaatat aactccgaaa gcaagcgtaa
     3361 aggccataga taagaagtgg taaacaacgg caaacgaaaa tgcttcaggt ttcgctatac
     3421 cgaaaatact taatcccagg atgcaggcaa agtgccagtt gccgataaaa ccgggagccg
     3481 tgggaatagc gataccgata ataagaacta tcattacaac aaacgaggcc ataaccggta
     3541 aggtaaaacc gaaagccaga aacaatacat aaatagacag cacatccaca agccagatca
     3601 gcgccgaaag aaaaaataaa taaaacagca ttttaacatt cttaataacc tgaaagccgt
     3661 caataaagtg atggatcaat tcatcaacct tatgagcaaa ttttccaggg agtttgctta
     3721 aaatccgatt gataattttt atggcagttt ctctgtgcca gattaaaccg gcaatacaga
     3781 aaaacatcag tattgtcaga agggcaaata aaatgcccga tttaatcatc caggaaggta
     3841 aatcagtgta gaaaagtaca accacagcaa ttgtcagtat ggaaaagcca tccagaactc
     3901 tttcgacgag aatagtgccc aaagccgaag acattttaat cgtgcttcgt ttggctatga
     3961 gatagggtct ggccagctct cccaggcgcg cgggaatagc ggcaatagct aggaatccga
     4021 ctgaggtcac ggaaaaaaga gaaaactgat cgatcttttg cagcggctgt agaataatcc
     4081 cccagcggta ggaacgaaga tattgcatta aaaaaacaat gccgatgaaa aaggcaacat
     4141 aactgagttg gatttttttc agatcatgga gagtatcctg gaagttgatt ccccgtaccg
     4201 ataagtaaat cagtataacg ctaagcacaa taccaaaaat tattttttta ttcatttgtc
     4261 tgcaagaccc cagccgtttt cctgttcccg atagctagtt tttggccagg ttctcggcaa
     4321 ttttgtcgtg gagaagctgg gaagagagct tgttacccat cagtcccacg cgaatggcaa
     4381 ctgttgtttg attttttgat ctgtatgtaa tatcgaattt gactttttcg tcatttatta
     4441 ctgtggtaat tcttccccgg gcaatttcct ttacgggagc tacttcaatt ccgcgcatat
     4501 ctgctactgt cttttcgcaa gagctccaga ctttgtcgaa tggggcaaaa tagtcgtttt
     4561 ttaattcacc gtcgacataa aaataagttc ctgtgcttgc tccaactgcc gctcctccaa
     4621 caaccaatgc ggcacaactg gaaaagataa aaagagcaaa taatgaaatc aaaagacaga
     4681 aaatatttct tttcaagttc ctccctccag ttaattcgta atgtatattt tagaacatat
     4741 tgcttcgggt attgcaatag
//
LOCUS       DASVIM010000022         3298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1459365, whole genome shotgun sequence.
ACCESSION   DASVIM010000022 DASVIM010000000
VERSION     DASVIM010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459365"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /gene="pssA"
                     /locus_tag="VF305_00410"
     CDS             complement(<1..430)
                     /gene="pssA"
                     /locus_tag="VF305_00410"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX7415630.1"
                     /translation="MNKETSLKKIRIKKGIYLLPNLITTASLFCGFYSIIASLKEHFV
                     VAAITILAALAFDGLDGRIARFTNTTSKFGAEYDSLSDVIAFGVAPSFLAYNWAMSFY
                     GKWGWLAAFLFVLCGALRLARYNIQIGMIESKFFNGLPIPA"
     gene            complement(427..1068)
                     /locus_tag="VF305_00415"
     CDS             complement(427..1068)
                     /locus_tag="VF305_00415"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEX7415631.1"
                     /translation="MKHDSIIAHEGYPFIIFSLVITVFVAFWGIIWLTILFALITFFI
                     IWFFRNPDRYFQEEERVLISPADGKIIKIEGVELNGNISGIFKKISIFMNVFNVHVNR
                     VPYSGKIETIDYHEGKFFSANLDKASLDNECNEVMIRADDGRSIWVVQIAGLIARRIV
                     CWINVGTPVKTGERFGLIRFGSRVDIYLPEDSRISVKLYDKVKAGETPLGYLS"
     gene            1250..2716
                     /locus_tag="VF305_00420"
     CDS             1250..2716
                     /locus_tag="VF305_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX7415632.1"
                     /translation="MSKFKWNITWGVFITLVFLILLFIRLEFFQKKPEPVPALQSPEE
                     VKAQNTWMNIYQNGKKIGIVHRIFTHQGKGFHFSEKVFMQINTIGVTQALHIFTEGNL
                     NSDMTLSSFNFNLNSSLFRFHAHGLVTKNKLILFTGLPDAQEKSEIPLKDIPRISGSI
                     YDTAFRANIENNVTRSFSIFDPSTLSIRSIKVARNADEIISSMGKQILAKKYCADFMG
                     AINCAWLDKNGDVLKETSILGLSMEKVSQKKAQEGISTSSTIDLTQIASVPSNVEIAE
                     PENLREIKIKISGISTTSLFLNGDRQSLRHDVLTITKEQLPAPQTLSNIPPEKIAVFL
                     KPAPLIQSSHQQIKGQVEKIIKSTDTAEQKARKIVNWVYRNIEKKPVLSVPNALEVLK
                     NKVGDCNEHAVLTAAMLRAAGIPAQIEAGLVFLHGRFYYHAWNVLYLDKWITADAVFN
                     QIPADVTHIRLIRGEGGEQLNLMSVMGKIKLEVLDQTK"
     gene            2713..>3298
                     /locus_tag="VF305_00425"
     CDS             2713..>3298
                     /locus_tag="VF305_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7415633.1"
                     /translation="MIELHELTKNYGNTIAVNKLSLNAAAGEIYGFIGPNGAGKTTTI
                     RMLGGILAPTSGNIIIGGLDMAKKPVAAKKMIGFVPDRPFLYEKLTGMEFLHFSADLY
                     DVNPDDFPQKAEQLLRQFALWDWANEIIEAYSHGMKQRLIIACALLHDPRILIIDEPM
                     VGLDPAAVHMVKDILKELSARQTTIFISTHTLSLA"
BASE COUNT         1047 a          709 c          651 g          891 t
ORIGIN      
        1 ctgcaggaat aggcaaaccg ttaaaaaact tactttcaat cataccgatt tggatattgt
       61 aacgagctaa acgcagcgcg ccgcaaagaa caaacaaaaa agcggcgagc cagccccatt
      121 tcccgtaaaa agacatggcc caattatagg caagaaaaga tggcgccacg ccaaaggcaa
      181 tcacatcgga cagtgaatca tattctgccc caaatttgct ggtggtattg gtaaagcggg
      241 ctattcggcc atctaatcca tcaaaagcaa gggcagctaa aatagtaata gccgctacaa
      301 caaaatgttc cttcaatgaa gcaatgatgg aataaaatcc gcagaataag ctggctgtgg
      361 taataagatt cggtaataaa taaattcctt ttttgatacg gatctttttg agtgaagttt
      421 ctttattcat gataaatacc ctaaaggtgt ttcacctgcc ttgaccttgt cgtaaagctt
      481 cactgaaatt cgggaatctt cgggcaaata aatatccact cgcgaaccaa aacgaatcaa
      541 gccaaacctt tcacctgttt taacaggcgt cccaacattg atccaacaaa caatccggcg
      601 ggcgataaga ccggctattt gcaccaccca aattgaccgg ccgtcatcgg cacgaatcat
      661 tacttcattg cattcattat ccagcgaagc tttatctaaa ttagcggaaa aaaatttacc
      721 ctcgtgataa tcaatggttt ctattttccc ggaataaggg acacggttaa cgtggacatt
      781 aaaaacgttc ataaaaatac ttattttttt aaatattccg gaaatattgc cgtttaactc
      841 aacaccttcg attttgatta tcttaccgtc agcgggagaa attaagaccc tctcttcttc
      901 ctgaaaatat ctatccggat tacggaaaaa ccaaataata aaaaaagtta tgagagcaaa
      961 taaaattgtc agccaaatga tgccccaaaa agccacaaaa acagtaatga ccagagaaaa
     1021 aattataaag ggataccctt catgagcgat gatgctatcg tgtttcataa agtatattat
     1081 ttttccttaa aaatggagct tcttttgata tatcaattgg gtgcacttgt caataaatct
     1141 ttgattctat aaaaagaata aatcataatg gctatgttgt tgactcttat ctgctccgtc
     1201 tatataatgc ctgacaaatt gtttattagt ctctggcagg ttagaaccta tgtcgaaatt
     1261 caaatggaat atcacctggg gcgtctttat tacacttgtc ttcctgatcc ttttgtttat
     1321 tcgccttgaa ttcttccaaa aaaaaccgga gcctgttccg gctcttcaat ccccggaaga
     1381 agtaaaagcg cagaacacat ggatgaatat ttatcaaaac ggcaaaaaaa ttggcattgt
     1441 tcaccggatt tttactcatc agggaaaagg attccatttt agtgaaaagg tatttatgca
     1501 gattaacacc ataggagtaa cacaggctct ccatattttc acagaaggaa atcttaattc
     1561 cgacatgact ctttcctcat ttaattttaa tttgaactcc agcctgtttc gtttccatgc
     1621 tcatggcctc gttaccaaaa ataaattaat cctgttcacc ggattgcctg atgcacaaga
     1681 aaaaagcgaa atcccgctca aagatattcc ccgtatcagc ggcagcatct atgacacggc
     1741 gttccgggct aatatagaaa ataatgtcac ccgcagtttc agtatttttg atccttctac
     1801 cctctccatc cggtcgatta aagttgccag gaatgccgat gaaattattt ccagtatggg
     1861 aaaacagatt ttggcaaaaa agtattgcgc cgatttcatg ggggccataa attgcgcctg
     1921 gctggataaa aacggagatg ttttaaaaga aacaagtatt ttaggccttt ccatggaaaa
     1981 ggttagccag aagaaagcgc aggaaggaat atcaaccagt tcaacaattg atctaaccca
     2041 aattgcgtct gttccttcca atgttgaaat cgctgaaccg gaaaatctca gagaaattaa
     2101 aatcaaaatt agcggtatca gcacaacgtc attattcctt aatggagaca gacaaagttt
     2161 gcgccatgat gttctgacca ttaccaaaga acaactgccc gcgcctcaga ctttgagcaa
     2221 tattccgccg gaaaaaattg ccgtcttttt aaaacctgcg ccgcttatac aatccagtca
     2281 ccagcaaata aaaggacaag tggaaaaaat catcaagtcg actgacaccg ctgagcagaa
     2341 agcaagaaaa attgtcaact gggtttaccg caatatcgag aaaaaaccgg ttttgtcagt
     2401 gcccaatgcg ctggaagttt tgaaaaataa ggtcggcgat tgtaacgagc acgctgtgct
     2461 gacggctgca atgctccgtg cggccggtat tccggcgcaa attgaagcgg gtttagtttt
     2521 cctgcacggc cgcttttatt accatgcatg gaatgttctc tacttagata aatggatcac
     2581 tgctgatgct gttttcaatc aaattcccgc cgatgtgacc cacatcaggc ttattcgcgg
     2641 tgaaggcggt gaacaactga acttaatgag tgtaatgggc aaaatcaagc tggaggtatt
     2701 agatcaaacg aaatgattga attacatgaa ctaacgaaga attatggtaa taccatagca
     2761 gtaaataaac tcagtttaaa tgctgccgcc ggtgaaattt atggttttat cggccccaac
     2821 ggtgcaggca agacaacgac aatccgtatg ctgggtggaa tccttgcacc cacctccggt
     2881 aacattatca tcggcgggtt ggatatggcc aaaaaaccag tcgcggcaaa aaaaatgatc
     2941 ggttttgttc ccgatagacc ttttctttat gaaaagctaa ccggcatgga gttcctccat
     3001 ttttcagctg atttgtatga tgtaaatcct gacgatttcc cgcaaaaagc tgaacagctg
     3061 ctgcggcagt ttgctctctg ggattgggct aatgagataa tcgaagccta ctcccacggt
     3121 atgaagcaaa ggctcattat tgcttgtgct ctactgcatg atcccagaat attgatcatt
     3181 gatgaaccaa tggtcggcct tgatccggct gccgtgcaca tggttaagga tattttaaaa
     3241 gaactgtccg ccaggcaaac gacgatcttt atttccacgc ataccttaag tctagctg
//
LOCUS       DASVIM010000023         3745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_435795, whole genome shotgun sequence.
ACCESSION   DASVIM010000023 DASVIM010000000
VERSION     DASVIM010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435795"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..545)
                     /locus_tag="VF305_00430"
     CDS             complement(111..545)
                     /locus_tag="VF305_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415634.1"
                     /translation="MTHLDKGKYFQKHSNQAKVDDSLRQEILQKAKENNISCAVAEEI
                     SQRKEMALGEVGMAIDLLNINIVECQLGLFGYSPHKKIVQPAKEVVPVLKENILAALD
                     NGRLPCIAAWEIAGKLNIPRMKVSAACEALQIKIKPCQLGAF"
     gene            complement(569..1021)
                     /locus_tag="VF305_00435"
     CDS             complement(569..1021)
                     /locus_tag="VF305_00435"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7415635.1"
                     /translation="MIAYQLSIFAENKPGKLAQVTGILAKEKISIRATTIATADTFGV
                     INLIVDGPKRAEAALAKAGMTVHLREVLAILIPDKPGGLDSLTQLFYQEGININNAYG
                     FVLESLKKAVFIVDVDQIQKTEKLLEQKGFKTLDTESLLAIEPGRQVK"
     gene            complement(1311..2609)
                     /locus_tag="VF305_00440"
     CDS             complement(1311..2609)
                     /locus_tag="VF305_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX7415636.1"
                     /translation="MYWEKDVETLNRAKLEALQLKALQDTIGRAQKSFYYGKLFKEKG
                     LNVNSVKSLNDINKFPFTNKDNLREHWPYGFIAVPKEELVRMHSSSGTTGRATVVFHT
                     ANDIAVWANLLARCMYMGGMRKSDVFQNMMTYGLFTGGLGFHYGAEKIGALVIHAGAG
                     NSKRQIQLMIDFETTAIHVIPSYALHLSIVFAELKVDPRKDTKVKIAFLGAEPHTEKM
                     RKKIEEIYGYKAFNSYGLSEMNGPGVAFECPYQNGMHIWEDSFLVEIIDPETLKPVAD
                     GKEGELVMTTLQREGMPLLRYRTKDLTKIIPEPCACGRTHRRIERIKGRTDDMLIIKG
                     SNIFPMQIEKKLMEISGVGTNFLIVLDREGFNDSLTVKVEVNKKYFSGDMKQLDALRK
                     KIVEELKSEILVTPKVDLVEPDSIPKGEGKAVRVIDNRKD"
     gene            complement(2708..3598)
                     /locus_tag="VF305_00445"
     CDS             complement(2708..3598)
                     /locus_tag="VF305_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7415637.1"
                     /translation="MKDRQFGRLRFVCGENRGKYPFNHSLYVESNEARVIIDPASSLE
                     KLSRLRDKDGVDMVWLSHWHEDHFHYVYLFGDSPLWISERDFPPLTDIEIFLDWYGVK
                     DDSHRDYWKKEMRERFNFQPQHKARFIGDDEKIDLGHVKVQVIATPGHTPGHLSFFFP
                     EEDVLFLGDYDLTAFGPWYGDVASSIDETIKSIRRLKEIPAKKWIACHEDGLFEENPG
                     KLWDNYENIIYDRERKILEFLKKPHTLEEISAAWLIYGKPRKPLEFFEFNERVLVNKH
                     LERLEKQGQIVRDKDQYRKV"
BASE COUNT         1035 a          880 c          718 g         1112 t
ORIGIN      
        1 gttgaaaaaa cctttaagca tgaaagaaat tatcgccggt actcgtaaaa atgccgattt
       61 aatgattcaa ttgctgcaaa gctacctgaa attatccgct ttaaaaaaaa ttaaaatgca
      121 cccaactgac atggcttaat ctttatttgc aaagcttcgc aggctgcgga aaccttcatg
      181 cgggggatgt ttaattttcc ggcaatttcc catgcggcaa tgcagggaag ccggccattg
      241 tccagcgccg ccaaaatatt ttctttcaaa accggcacaa cttctttggc cggctgcaca
      301 atttttttat gcgggctata accaaaaagc cccaattggc attcgacaat attgatattt
      361 aaaagatcaa tggccatgcc aacttcgccc aaggccattt cttttcgttg ggaaatttct
      421 tccgcgactg cacaggaaat attattttct tttgcctttt gcagaatttc ctgccttaaa
      481 gaatcgtcaa ctttagcctg attcgaatgc ttttgaaaat atttgccttt atctaaatgt
      541 gtcataatct ttttcctcta tttaaaactt attttacctg acgaccaggc tcaatcgcca
      601 aaagcgattc cgtatccagc gttttaaatc ccttctgctc caaaagcttt tccgtttttt
      661 gaatttggtc gacatcaact ataaataccg cttttttcaa gctctccagc acaaaaccat
      721 aggcgttgtt aatgttgatg ccttcctgat aaaacaactg ggtaagacta tcgagaccgc
      781 ctggtttgtc cggaataaga atcgccagca cttcgcgcaa atgcacagtc attcccgctt
      841 tggccagggc ggcttccgcg cgcttcgggc catcaacaat cagattgata acgccgaaag
      901 tatccgctgt cgctatcgtt gtagcccgga tacttatttt ttctttggcc agaataccgg
      961 ttacctgcgc aagttttccg ggtttatttt ccgcaaaaat agaaagctga taggcaatca
     1021 taattttctc ctttgtttat ttcaaaatat tacttcggca gtttaaatat tttaccagaa
     1081 aatttcaagt ggcttagcct tagctttacg ccatcactgg attccggcct tcgccggaat
     1141 gacggcattt agctacttta ttgtctgagt aataatatct tacacacacc tttgatcttc
     1201 aatgtagaac gaggcggcaa ggaggaggct ccgcaggcgt atatggaaat acgttgagga
     1261 agccgacgac gctgccaaca aagttatacg aagaagagca aagttgtgtc ctagtctttg
     1321 cggttgtcaa tgaccctaac cgccttccct tctcctttcg gaatgctgtc cggctcaacc
     1381 aagtcaactt ttggcgtaac cagaatttcg ctttttaatt cttcgacaat tttctttctt
     1441 aaagcatcca attgtttcat gtcgccggaa aaatattttt tattcacctc tactttcacc
     1501 gtcagggaat cgttgaatcc ttcacggtct aagacaatta agaagtttgt gcccacgccg
     1561 gaaatttcca tcagtttctt ttcaatttgc atgggaaaaa tattggaacc tttgatgatc
     1621 aacatatcgt cagttcttcc cttgatgcgc tcaatccgcc gatgggtgcg gccgcaggcg
     1681 cagggctccg gaataatttt ggttaaatcc ttggtgcggt aacgcaatag aggcatgccc
     1741 tctctctgta acgtcgtcat taccagttcg ccctctttgc catcggcaac cggctttaaa
     1801 gtttcaggat caataatctc cactagaaaa ctatcttccc agatatgcat accgttttga
     1861 taaggacatt caaaagccac acctggcccg ttcatttcgg ataggccata agaattgaat
     1921 gctttatatc cgtatatttc ttcaatcttc ttgcgcatct tttccgtatg cggctccgcg
     1981 cctaaaaaag cgatttttac tttggtgtct ttacgcggat caactttaag ttcggcaaaa
     2041 acaatggaga gatgcaaagc atagctggga atgacatgaa tcgcggttgt ttcaaaatca
     2101 atcatcaatt gaatttgacg cttggaattg cccgcaccgg catgaataac cagagcgccg
     2161 attttttccg cgccgtaatg aaagccaagg ccgcccgtaa aaaggccgta agtcatcatg
     2221 ttttgaaaaa catcgctttt gcgcataccg cccatataca tacagcgcgc cagaaggttt
     2281 gcccaaacgg cgatatcatt tgcggtatga aacactaccg tggctctccc ggtagtccct
     2341 gaagatgaat gcatccggac aagttcctct ttcggcacgg cgataaagcc gtaaggccag
     2401 tgttcgcgca aattatcttt attggtaaaa ggaaatttat taatatcgtt gagagatttt
     2461 actgaattta cattcaatcc tttttcttta aatagtttac cgtaataaaa agatttctga
     2521 gccctgccta tggtatcttg gagagccttc agctgcaaag cctccagctt ggcgcgattc
     2581 agtgtctcta catctttttc ccaatacatt taactcctcc agctattgtc aatgagtttt
     2641 ttacaacccg gccaattttt tatcaagtca aattaaattt ggtgaatatt tattgaaaaa
     2701 attaaattta aactttccgg tattgatcct tatcgcggac aatttgtccc tgtttctcca
     2761 aacgctccag atgtttattc acgaggacgc gctcattaaa ttcaaagaac tccaaaggtt
     2821 ttcggggctt tccataaatc agccaggcgg cggaaatttc ttccagtgta tgcggttttt
     2881 ttaaaaattc cagaattttc ctttcgcggt catagataat gttttcgtag ttatcccaga
     2941 gcttccccgg attttcctca aacaaaccgt cttcatgaca ggcaatccac tttttggccg
     3001 gtatttcttt caggcgccgg attgatttga tggtttcatc aatactggaa gcaacatcgc
     3061 cataccaggg gccgaatgct gttaaatcgt aatcgcccaa aaagagaacg tcttcttcgg
     3121 gaaaaaagaa agacaggtgg ccgggggtat gtcccggggt ggcaattact tgaaccttga
     3181 catgccccag atcaatcttt tcgtcatccc cgataaagcg cgccttatgt tgcggttgga
     3241 agttaaatcg ctcccgcatt tcttttttcc aatagtcgcg gtggctgtcg tctttcacac
     3301 cataccaatc cagaaaaatc tctatgtccg tcagaggcgg aaaatcccgc tcggaaatcc
     3361 acaaaggtga gtccccaaaa agatatacat aatggaaatg atcttcatgc cagtgagaaa
     3421 gccagaccat atccacgcca tccttatcgc gcaggcgaga tagcttttcc aaactgctgg
     3481 ccggatcgat gataacgcga gcttcattac tttcaacata aagtgaatga ttaaacggat
     3541 atttgccacg gttctcgccg caaacaaaac gcaggcggcc aaattgacga tctttcataa
     3601 aaacctaatt aaaattatcg tcacaaaata tatgaaatta tccgcacaat ttccataatt
     3661 aattgtcaca aactttataa tgaacaccac ttgaaataaa ttatgccttc attcccaaac
     3721 agtagtatcg gacaaaatgc attga
//
LOCUS       DASVIM010000024         3530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_351049, whole genome shotgun sequence.
ACCESSION   DASVIM010000024 DASVIM010000000
VERSION     DASVIM010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351049"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VF305_00450"
     CDS             <1..494
                     /locus_tag="VF305_00450"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX7415638.1"
                     /translation="GMGTTIAAALMIDNKLSIAHIGDSRVYLIRSDNIEQLTDDHSIV
                     NEQIKRNIITKEDAAKSTIKNLLTKALGISPEAEAELNELTLFDGDILLLCSDGLNTM
                     LTDEAILKIISSAKRPIDACDFLINAANEKGGKDNITVIVGYIKKKNWHSAFNKFTES
                     LWR"
     gene            498..1256
                     /locus_tag="VF305_00455"
     CDS             498..1256
                     /locus_tag="VF305_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7415639.1"
                     /translation="MTRILLKYKKTVIRDIAMDKKKLMTIGRKSDNDIFIDNQAVSGH
                     HAVIKIEGDGLFIEDLNSLDGTYLENDGLFIEDLNSLNGTFLNGHKVSKGQLFHGDVI
                     LIGVHTLEVISDKIRGKDKKSFTVRGHSMEETVVITRDDKNKILISADKSIQDPLGGF
                     IVIEGSTDSKEYQFKDRVSAIGKEKGSAIKLKGLFAPKVAALVNRRKEGYFITPSSNK
                     KLKINGKEIDHRYDLKDGDIVEVAKLKLQFYVKE"
     gene            1279..2085
                     /gene="murI"
                     /locus_tag="VF305_00460"
     CDS             1279..2085
                     /gene="murI"
                     /locus_tag="VF305_00460"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX7415640.1"
                     /translation="MNTITNRPLGVFDSGIGGLTVVRALMERLPFENIIYFGDTARVP
                     YGTKSVENINNYAAQITEFLIKKDVKLLIVACNTMAAVAYQTIKDLSSVPVLEVIDAS
                     ARIAAKDTRTKSIGVIGTPATINSNAYARAIRLLEKEAKIFSQACPLFVPLVEEGWFD
                     HPATRLIAQEYLKPVIAEQIDTLVLGCTHYPLLKPLLQDIMGPQVKLIDSAEAMADIA
                     ADLIDKQNLGNQNHRQPDYLFYVSDLPSRFQTIGERFLERTLRHVELVCL"
     gene            2193..>3530
                     /locus_tag="VF305_00465"
     CDS             2193..>3530
                     /locus_tag="VF305_00465"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX7415641.1"
                     /translation="MKILLAGYNLDYDLIRELKDKSALKQDLTPETISAAYARISRSP
                     KPVNELREITREEVDKARLSNRNIVFEMGHSSVAEHAVFNIDVLGVSRLLVEEIEKFR
                     LCSFTEKSQRYVLFDKDFVVPEEIKQAGMAGLFTATIMTQNDYYHKLYEKLKPHIVSK
                     NKDLAKNPSNRSLLEGWAKEDARYVISLATQTQLGMTINARNLELMLRRLAALPLAEA
                     QEYSKKLYTATKDIAPSLIRYTQATDYDRLTRQNLRRQASAFLPKHSPQSDVPKKSSA
                     VQLTFFSPAADAKVTATLLFSSSNLNYALCLRQASRMTLREKKALFKTAFQHLQAHDA
                     VLRELENVDLQFELLVSASCFAQLKRHRLATIISQEYDPQLGTTIPPSVREIGQQKEF
                     IEIMRLTRSVYDQIKKKTPLAAAYILTNAHRKRVLMKFNARELYHLARLRADGH"
BASE COUNT         1086 a          767 c          759 g          918 t
ORIGIN      
        1 aggggatggg aacaactatt gccgcagcgc taatgattga caacaaattg agcatcgccc
       61 atattggtga cagccgcgtt tatttaatca ggtcggacaa catcgaacag ttgaccgatg
      121 accattcaat tgttaatgaa caaattaaac gcaatataat cactaaagaa gatgcggcaa
      181 aatccaccat taaaaattta ctcactaaag ctctgggaat cagcccggaa gcagaagcag
      241 aattaaatga acttacctta tttgatggtg atatactact tttgtgtagc gatggcctga
      301 acactatgct aactgatgaa gccatactta aaataatatc ttcggcgaaa agaccaatcg
      361 acgcatgcga ttttttgatt aatgcagcta acgaaaaagg cggtaaagat aatattactg
      421 ttattgtagg ttatattaaa aagaaaaatt ggcattcggc atttaataaa tttacagaat
      481 ctctctggag gtaatgtatg acaagaatat tattaaaata caaaaaaacg gtaattagag
      541 atattgccat ggataaaaaa aagttgatga ctatcggcag gaaatcggac aatgatattt
      601 ttatcgataa tcaggccgtc tccggacatc acgctgtgat aaaaattgaa ggcgatggtc
      661 tgtttatcga agatttaaac agccttgacg gcacatactt agaaaacgat ggtctgttta
      721 tcgaagattt aaacagcctt aacggcacat tcttaaacgg tcataaagta tccaaaggcc
      781 aactttttca tggtgatgtc attttaattg gcgtgcatac attagaggtt atttccgata
      841 aaataagggg aaaagataaa aagagtttta ctgttcgcgg tcactccatg gaagaaaccg
      901 ttgttataac tcgggatgat aaaaataaaa tattgatctc tgccgataaa agtatacaag
      961 atccgctggg aggatttatc gtcattgaag gatcaactga tagtaaagag taccaattta
     1021 aagatagagt atcggcaatc gggaaagaaa aaggttcggc cataaaactt aaaggtcttt
     1081 ttgctcccaa agtagccgcg ttggtcaacc gccgcaaaga aggttatttt attacaccct
     1141 ccagcaacaa gaaattgaaa ataaacggca aagaaattga ccaccgctat gatctaaaag
     1201 acggcgatat tgtggaagtg gccaagctta aattacaatt ttatgttaaa gagtaaaagt
     1261 ctctttgcaa gagaatccat gaatacaata acaaatcgtc ccctaggtgt gtttgattcc
     1321 ggtattggcg gcctgactgt agttcgcgca ttgatggaac gtctgccttt tgaaaatatt
     1381 atttactttg gcgatactgc gcgtgttcct tatggaacaa agtcggtcga aaacatcaat
     1441 aactacgctg cgcaaatcac cgaatttctc atcaagaagg atgtgaaact tttgatcgtt
     1501 gcctgcaaca caatggcggc agtggcctat cagacgataa aagatttgtc ttctgtaccg
     1561 gtactggaag taattgatgc cagcgcccgc attgccgcca aagatactcg caccaaatcc
     1621 atcggagtca ttggaacacc ggcaacaatc aacagtaacg cttacgcccg cgctatccgc
     1681 cttttggaaa aagaggcaaa gattttttcg caagcttgtc cgctttttgt gccactggtg
     1741 gaagaaggct ggtttgatca tccggcaacg agactgattg ctcaggaata cttaaagccg
     1801 gtgattgccg agcaaattga cacgctggtt ttgggctgca cccattatcc gcttttgaaa
     1861 ccgttgctac aagatattat ggggccgcag gttaaactaa ttgactcggc ggaagcaatg
     1921 gccgatatcg ccgctgactt gatcgataaa caaaacctgg gcaaccaaaa tcaccggcag
     1981 cccgattatc ttttttatgt aagcgattta ccttcccgct ttcaaacgat cggcgaacgt
     2041 tttctggaac gcacgcttag gcatgttgaa ttagtatgcc tttaaatgat ttatcattcc
     2101 cgcccggcac gtgtgacctc tcgtgcctac agggtatcag gttatgcgcc aggtcaaact
     2161 ccggcgggaa gtcatgaata ctgaataact ttatgaaaat tcttctggcc ggctacaatt
     2221 tggattacga tcttatccga gagctaaaag acaaaagcgc tctgaaacaa gatttaacgc
     2281 cggaaaccat atcggcagcg tatgcgcgca tcagccgcag tcctaaaccg gtcaacgaat
     2341 tgcgcgagat cactcgcgaa gaagtggaca aggcgcgcct ctccaaccgc aacatagttt
     2401 ttgaaatggg gcacagctcc gttgccgaac acgccgtatt taatattgat gtgctgggtg
     2461 tttcccggtt gctcgttgaa gaaattgaaa aattccgttt gtgttctttt acggaaaaat
     2521 cacaacgtta tgttctgttt gataaggatt ttgtcgtccc cgaagaaatc aaacaggccg
     2581 gcatggctgg tttgttcacc gcgacaatca tgactcagaa tgattattac cataagctct
     2641 acgaaaaatt aaaaccccac attgtttcaa agaacaagga tttggcgaaa aacccgtcca
     2701 accggtcact tctggaagga tgggccaagg aagatgcccg ctatgtaatt tcgctcgcta
     2761 cccaaaccca actgggcatg acaattaatg cccgcaacct ggagcttatg ctgcggcggt
     2821 tggccgctct gcctcttgcc gaagcgcagg aatacagtaa aaaattgtat acagcgacaa
     2881 aagatatcgc accttctctt atccgctata ctcaggctac agactacgac cgcctgacaa
     2941 gacaaaattt gcgtcgccag gcctctgctt ttttaccaaa acattctcct caatcagatg
     3001 ttccaaaaaa atcatctgcc gtacagttga cttttttttc tcccgctgcc gatgccaaag
     3061 tcaccgccac tttgctgttt tcttcgtcca atttaaatta tgctctgtgc ctgagacagg
     3121 cgagccggat gacgctgaga gaaaaaaaag ccttgttcaa gacagctttt caacatctac
     3181 aagcgcacga tgcggtgctg cgggaattgg aaaatgtcga tctgcaattt gagcttttgg
     3241 taagcgccag ttgttttgcg caattaaaaa gacaccggct ggccacaatt atatctcaag
     3301 aatacgaccc gcaattaggc acaactattc ccccttctgt acgcgaaatc ggccagcaaa
     3361 aagagtttat agaaattatg cgccttactc ggagcgttta tgaccaaatc aagaaaaaaa
     3421 cgccccttgc cgctgcttat attttgacta atgcacaccg caaaagagtg ctgatgaaat
     3481 tcaatgcccg cgaactatat catctggcgc gtttgcgcgc tgatgggcac
//
LOCUS       DASVIM010000025         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1205132, whole genome shotgun sequence.
ACCESSION   DASVIM010000025 DASVIM010000000
VERSION     DASVIM010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205132"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1461
                     /locus_tag="VF305_00470"
     CDS             166..1461
                     /locus_tag="VF305_00470"
                     /inference="COORDINATES: protein motif:HMM:NF018271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415642.1"
                     /translation="MMNHTMLDDGMSVFYYVALFAIYSFLGWVIEVIYRSITQRKFVN
                     AGFLYGPFIPIYGISAVFIIILQYFFHTWHLVPRFIIFGLMLTAIEYAVGFSSEKIFK
                     LTLWDYSEYKFNLHGRVCLYFSVFWTALALAFVTFIHPVVFSNVITLDALYIKAAVVT
                     FLIYYLIDFAFSVISITEFQKKIAYLYTEYFNLSNVEIERILNSFQRLRNAFPDLNRY
                     INKNINNKIKNRINTFLKSIQDKIILEINGRKPFEHEYYEIIQDIDGHVDFMKLKDYF
                     HHNSSIYAHVQDVAYLSYRICKFLKLDYQSAARGALLHDYFLYDWRNHDVPDLPRGKF
                     HGIEHPTIALANAQKHFFLNEIEEDIIKKHMWPLTFFVPKYKESFIVSFADKYLSSKE
                     FIGEFKKRKSYRKAQKTHRRHNKSSTKSGKNWTDQHRLR"
     gene            complement(1725..>2544)
                     /locus_tag="VF305_00475"
     CDS             complement(1725..>2544)
                     /locus_tag="VF305_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX7415643.1"
                     /translation="LHGKSFIPMCLGFGCNVPAVLGARIIETRKARMITLLLIPFVPC
                     TARLAVLTLVSAAIFGTNAVYVSWSILSLNIIVLGLAGALVNKTLWQQDAPFIMELPI
                     YHSPDPRTIMMVVWSRTISFIRKAGTIILAVSLLIWLLSYFPSGVVEESFLASLGKFL
                     QPLGVPLGLDWKMITALLTGLVAKENVVATLGVLYSVGAKGLVNVLPTVMSHASAAAF
                     LVVMMLFIPCAATIAVLKKEMNSNKWFYSTIVMTLIVSYLGGIAAYNFVKWLGL"
BASE COUNT          786 a          563 c          501 g          694 t
ORIGIN      
        1 aaacttcatc cgcagaaatc tttttctcta ccgtcccgtt gtaaaaaata taagctactg
       61 aaatacatat tactctttgc aaaaacgctc agttgtgata aaaaatcatt accacgacaa
      121 tgttcttaaa tatttaactg ccggatataa aattatttat gtgtgatgat gaaccatacc
      181 atgctagatg acggcatgag cgtattttat tatgtggcgc ttttcgcgat ctattccttc
      241 ctgggatggg tcatcgaggt tatttaccgt tcaataaccc agcggaagtt tgtcaatgcg
      301 ggatttttat acgggccttt cattcccatc tacggcataa gcgcggtttt tataatcatt
      361 cttcaatatt ttttccacac ctggcatctt gtgccccggt ttattatatt cggcctcatg
      421 cttaccgcca ttgaatatgc cgttgggttt tcttctgaaa agatatttaa attgacgtta
      481 tgggattatt cggaatacaa atttaacctc catggaaggg tgtgccttta tttttcagta
      541 ttctggacag ccctcgccct cgccttcgta acatttattc atcccgtggt ctttagcaat
      601 gtaatcacac tggacgcctt atatatcaaa gccgcagtgg tcacttttct aatctactat
      661 ttaattgatt ttgcgttttc agtaatatct attacggaat tccaaaagaa aattgcctat
      721 ctgtatactg aatactttaa cctgagcaat gttgaaatcg aaaggattct taattctttt
      781 cagcgcctgc gcaatgcatt cccggatttg aacaggtata tcaataagaa tatcaacaat
      841 aaaattaaaa acagaatcaa tactttttta aaatccatcc aggataagat catcttggag
      901 ataaatggcc gcaagccatt tgaacatgaa tattacgaaa taattcaaga catagacgga
      961 cacgttgatt tcatgaaact caaagattac tttcatcaca attcttcgat ctatgcgcac
     1021 gtgcaggacg ttgcttattt atcttaccgg atatgcaaat ttttaaagct ggactaccag
     1081 tcggcggcgc gcggcgctct cctccatgat tattttctgt atgactggcg caatcacgat
     1141 gtgccggacc tcccccgggg aaaatttcat ggcatcgaac atcctacgat tgcccttgct
     1201 aatgcccaga aacacttttt tctaaatgaa attgaagagg acattatcaa aaagcacatg
     1261 tggccgctca ctttttttgt gcccaaatat aaagaatcct tcatcgtttc atttgccgat
     1321 aaatatcttt cctcaaaaga atttatcggt gaatttaaaa agagaaaaag ctataggaaa
     1381 gcacaaaaga cacacagaag gcataataaa tccagcacga aaagtggaaa aaattggact
     1441 gatcaacacc ggttacgctg ataaagaggc tcttatagct accgcaaaaa aattcgagtt
     1501 cgatattcaa agtagaacac ggcagcatgc gtgatttttt tgtttatttt tcgatctggt
     1561 ttagcttaag cacttataat tcaactttca aatcaaagat gatatcgagg cgacaaggag
     1621 aaggcttcgg aggcgtattt aaatatacgt tgaagaagcc gacgatccgg catacgccgg
     1681 acaggctccg ccaacaaaga tagcgctttg agttagaagt tgaatcagag acccagccat
     1741 ttgacaaaat tatacgccgc aatcccgcca agataggaaa cgatcagtgt cataacgata
     1801 gtggagtaaa accatttatt gctgttcatt tcttttttca acacggcgat tgttgcggcg
     1861 cagggaataa aaagcatcat caccacgagg aaggccgcgg cagaggcgtg gctcatcacc
     1921 gtcggcagaa cattaaccaa accttttgcg cctacagaat aaagaacgcc cagtgtagcc
     1981 acaacattct ctttagcgac aagaccggtt aaaagagcgg tgatcatctt ccaatccagc
     2041 cccagcggca ctcccagcgg ctgtagaaat ttccctaatg aagccagaaa actttcttcc
     2101 acaacgccgg aagggaaata agataaaagc cagatcagca aggaaacggc aagaataatc
     2161 gttcccgctt tacggatgaa ggaaattgtt ctggaccaga ccaccatcat aatggttctt
     2221 gggtcgggac tatggtaaat cggcaactcc ataataaaag gcgcgtcctg ctgccagagt
     2281 gtcttattca ccaaagctcc cgccaatccc agaacgatta tgttcagaga cagaatgctc
     2341 caggatacat aaacagcgtt ggttccaaaa atagcggcag aaacaagtgt caataccgcc
     2401 aaccgcgccg tgcagggaac aaaggggatt aaaagcaaag taatcattct ggctttgcgc
     2461 gtctcaataa tccgtgcacc cagaacggca ggcacgttgc agccgaatcc caggcacatc
     2521 ggaataaagc tttttccatg caga
//
LOCUS       DASVIM010000026         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_266195, whole genome shotgun sequence.
ACCESSION   DASVIM010000026 DASVIM010000000
VERSION     DASVIM010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266195"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..885)
                     /locus_tag="VF305_00480"
     CDS             complement(49..885)
                     /locus_tag="VF305_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX7415644.1"
                     /translation="MLNLVVCLKQVPMVTELPWDEKTGTLRRDLAAGMMNPACKHALE
                     AALQIKEKYSANITAITMGPPAAQEIMREALALGADRAILICDKLLAGSDTFATSWIL
                     AEAIKKECPKFDLLLCGAYTSDSETAQVGPQLAEKLDLPAAAYIEKLEINGRTLRVRR
                     LVDNFRETLEMEFPALVTVSMENYEPRYTKLVGLENSFNENSVIILDAGALGINASVM
                     ENQGSRTKVRKVFLRKAQKENVLLQGTAATVVTEFLNKYQRKISTAIGKSIEDNKQTH
                     EE"
     gene            complement(892..2025)
                     /locus_tag="VF305_00485"
     CDS             complement(892..2025)
                     /locus_tag="VF305_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7415645.1"
                     /translation="MKHKLIRNSVRHFAETELAPIAAEIDRNAIFPHDVIEKMKKLNY
                     FGLQVPKEFGGAAMDSISAAIVVEEISRVCAAVGLCVTVHNGVAVYPFLQYATPEQKD
                     TYLTRLASGEIIGAFSLTEANAGSDAGSVETTAVKKGHEYILNGTKIFVTNGGICDLS
                     LIFALTNTDQTRLQSSVFIVESGFPGFLRGELEDLCGMRANPVSSLFLEDCRVPEKNL
                     LGKLGDGMKIGLATLDNGRIGVAAQALGIAQGAFEAAVKYAKERQQFKKPIALLQTIQ
                     NYIADMATEQAVARLLLYRACSLKDEGSPFGCEAAMAKLYCSQVASKTTSLAVQIHGG
                     YGYSKEYDVERYFRDAKVTEIYEGTSEIQRMVIARSVLSKLIL"
     gene            2204..2563
                     /locus_tag="VF305_00490"
     CDS             2204..2563
                     /locus_tag="VF305_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415646.1"
                     /translation="MKIRILLFSFVLLFAYLPAAHTQEYGKIRALQQRAAYVTKQKND
                     FVARVLTSYKIPHERNTQGVVVRINMEGKWLSIQTIEIVPVIKEAADKSQYVIAHELF
                     FYTDKNILDLVSELTIR"
     gene            complement(2568..2891)
                     /locus_tag="VF305_00495"
     CDS             complement(2568..2891)
                     /locus_tag="VF305_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415647.1"
                     /translation="MPESMQITIGILALLAVYLLAMLGTGWWTKQICLSIIRELEDKG
                     AVNAGSAVDLPYAKVNYFKIGYRDYRPKALETLILSEVVCKTFQGRYYLSKEKLAETQ
                     NNTNV"
     gene            3099..>3469
                     /locus_tag="VF305_00500"
     CDS             3099..>3469
                     /locus_tag="VF305_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415648.1"
                     /translation="MKKDKTLKKKTPIKKAKTVKKAKVLEKTKAVKRVQAPKKAAVKK
                     VKAVKKAGAPVKALVKKVKAPTKTPVEKVKTPLTPALQDLQKLKVKFTLHPYQYEEPG
                     GTKLSAMKLGVDEHIVIKTLI"
BASE COUNT          992 a          794 c          712 g          971 t
ORIGIN      
        1 ttttgataat agttgcggta atcgccgaat acccagatgc tttttttctt actcttcatg
       61 agtctgttta ttgtcctcta tagatttgcc tattgccgtg ctgatttttc tttgatattt
      121 attgagaaat tccgtaacca cggtggccgc cgttccttgc aaaagcacgt tttctttctg
      181 tgcctttctt aaaaaaactt tgcgcacctt ggtgcgggag ccctgatttt ccattacgga
      241 agcgtttata ccgagagctc cggcatcaag aataataacg ctattttcgt taaacgaatt
      301 ttccaaacct accagcttcg tgtagcgtgg ttcataattt tccatggaga cagttaccag
      361 cgccggaaat tccatttcca gcgtttcgcg aaaattatcg acgagtctgc ggacacgcaa
      421 cgtccggccg ttaatttcca gtttttcgat atacgccgcg gcgggtaaat ctaatttttc
      481 agctaattgc gggccgacct gtgccgtctc gctgtctgag gtataagcgc cacaaagaag
      541 cagatcaaat ttcggacact ccttttttat cgcttcagcc aaaatccagg aagtagcaaa
      601 ggtatcggag cctgccaaaa gcttatcgca aataaggata gcacgatccg cacctaaagc
      661 cagcgcttcg cgcataattt cctgggcggc cggcggcccc atagttatcg cggtaatatt
      721 cgctgaatat ttttctttta tttgcaaggc ggcttccaga gcgtgtttgc aggctggatt
      781 catcattccg gccgccagat cgcgccgcag cgtgccggtt ttttcgtccc acggcagttc
      841 cgtcaccata ggaacttgtt ttaagcaaac taccagattc aacatatttc tctataatat
      901 caattttgac agcactgaac gcgcaatgac catgcgctga atttcgctgg tgccttcata
      961 aatttcggta actttagcgt cacggaaata tctttcgaca tcatattcct tgctgtatcc
     1021 gtaaccgccg tgaatttgca cggccagtga ggtggtcttc gaagcaacct gactgcaata
     1081 aagtttcgcc atcgccgctt cacagccgaa aggcgagcct tcgtccttca aggaacaggc
     1141 acggtacaaa agcaggcgcg ccacggcttg ttctgtggcc atatcggcaa tgtaattttg
     1201 aattgtttgc aataaggcaa tcggcttttt aaattgctgc ctttccttgg cgtatttgac
     1261 cgcagcttca aaggctccct gcgcgatacc cagtgcttga gccgcaacgc ctatgcggcc
     1321 gttatccagc gtagcaaggc ctattttcat cccatcgccc agtttcccca aaagattttt
     1381 ttccggcaca cggcaatctt ccaaaaacaa tgaggaaacc ggattggcgc gcataccgca
     1441 aagatcttcc agttcaccgc gcaaaaaccc gggaaagccg ctctccacaa taaacacact
     1501 gctttgcagg cgtgtctgat cggtattggt aagagcaaaa attaaggaca gatcgcaaat
     1561 tccgccgttg gtgacaaaga ttttcgtgcc gtttaagatg tattcatggc ctttttttac
     1621 cgcggttgtt tccacactgc ccgcatcaga accagcgttg gcttcggtca ggctgaaagc
     1681 gccgataatt tcaccgctgg ccagccgcgt caggtaagta tctttttgtt ccggtgtcgc
     1741 gtattgaaga aacggatata cagcaacgcc attgtgcaca gtaacgcata agccgaccgc
     1801 ggcacaaacg cgggaaattt cctccacaac tattgccgcg ctgattgaat ccatggccgc
     1861 cccgccaaat tccttgggca cttgcaggcc aaaataatta agctttttca ttttttcaat
     1921 gacatcgtgg ggaaatatag cattgcggtc tatttcggcg gcaataggcg caagttcggt
     1981 ttccgcgaaa tgccgcacgg aattacgaat aagtttatgt ttcatgcaag gattgaattg
     2041 caacttacgt ctcccggatc aaagtgctat tttttcacaa ataacctaaa ataattgtaa
     2101 aatcaagaaa atgaagcaaa aaaactttat ctggcatatc aaaataagaa tctatacaat
     2161 tatactatat taacaatgat gccttttaga aaaggagaat attatgaaaa tacgtattct
     2221 attgttttca tttgttttat tgtttgctta tctgcctgcc gcacacactc aggaatatgg
     2281 caaaattcgc gctctgcaac agcgagcggc ttatgtgacc aaacaaaaaa atgatttcgt
     2341 tgcccgggtt ttaacatctt ataaaatccc tcatgaacgt aacactcaag gcgttgttgt
     2401 gcgcataaat atggagggaa agtggctgag tatccaaact atcgaaattg ttcccgtgat
     2461 caaagaagcg gctgataaga gccagtatgt tatcgctcat gagttatttt tttataccga
     2521 caagaacatt ctcgatttgg tttcggagtt gacaattcgc tgacttttta aacatttgtg
     2581 ttattttgcg tttcagccaa tttctctttg cttaaataat accggccttg aaaagttttg
     2641 caaactactt cgcttaaaat aagggtctcc agcgctttgg gacgataatc gcgataacct
     2701 attttgaaat aattaacttt agcgtacggc aaatcaacgg cgctgcccgc attcaccgcc
     2761 cctttgtcct caagttcccg gataatggaa aggcatattt gttttgtcca ccagccggtt
     2821 cctaacatag ccaaaagata aacagcaagt aaagccaaaa taccgattgt tatttgcatt
     2881 gattccggca tgaaatacgt ccttgttttg aatagtttat atcatcttta aacaatttat
     2941 tttaattgag caaggattta attgagaatt gttccctttg attatctcgt ctttgattct
     3001 taccctgaaa tcataagata ttgctttttt tgttataatt gttattataa cgttgtaaga
     3061 accatgaaaa tgataaaatc ctgaaaagag agggacatat gaaaaaagac aagaccctga
     3121 aaaaaaagac gcccataaag aaagctaaaa ccgtaaaaaa agctaaagtt ctggaaaaga
     3181 ctaaagccgt caaaagagtc caagctccaa aaaaagcggc tgtaaaaaaa gttaaggctg
     3241 taaaaaaagc tggcgcaccc gtaaaagctc tggtgaaaaa agttaaggcg ccaacaaaaa
     3301 cacccgtaga aaaagtcaag actccgttaa cacctgccct acaagatttg caaaagctaa
     3361 aggttaagtt cacattgcat ccgtatcaat acgaagaacc gggcggcacc aaactatcgg
     3421 caatgaaatt gggagttgac gagcacatcg tcataaaaac gctgatcat
//
LOCUS       DASVIM010000027         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_949651, whole genome shotgun sequence.
ACCESSION   DASVIM010000027 DASVIM010000000
VERSION     DASVIM010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949651"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VF305_00505"
     CDS             complement(<1..484)
                     /locus_tag="VF305_00505"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7415649.1"
                     /translation="MDYRSLGKTGLKVSEVGFGGIPIIRLQTDEAVKVLLHAYDKGIT
                     FYDTANMYRDSESKIGLALSSFRDKIVIATKTTLRDAAGFRKHLERSLKMLKTDYIDV
                     YQFHQVANDEEWGKITKDNGALSAADKAKKEGKIRFLGVTSHNLQMAVKLVKTGLFDT
                     I"
     gene            complement(491..985)
                     /gene="lspA"
                     /locus_tag="VF305_00510"
     CDS             complement(491..985)
                     /gene="lspA"
                     /locus_tag="VF305_00510"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX7415650.1"
                     /translation="MKRNLIIFIIGAIAVIALDQITKAAITEKLFMYGSHKVIDGFFN
                     LVYVTNPGAAFGFLAGAPEIFRYLFFIGITVLAILLIIYYIMKSKQETLLIVISLALI
                     FGGAVGNLIDRIRFGAVVDFLDFYIGTWHWPAFNVADSAISVGAVLMIWKMLVNRRKT
                     SLSR"
     gene            complement(1008..>2764)
                     /locus_tag="VF305_00515"
     CDS             complement(1008..>2764)
                     /locus_tag="VF305_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7415651.1"
                     /translation="KYGLENYAPVDDNGKFTDDVEHFAGQFVFDANDNVIKKLKEVGA
                     LLGHFPLEHSYPHCWRCKNPIIFRSTEQWFISMEKNDLRKKALACIDQVEWIPSWGHD
                     RIYGMVENRPDWCISRQRLWGVPITVFYCGKCKAEVLTKKMLDYLVKLVEAGGADIWF
                     EKEAQDLMPKGTTCPHCKSSEFIKETNILDVWFDSGVTHAAVLETRKDLHSPADMYLE
                     GSDQHRGWFHSSLLESVGTRGRAPYLSVLTHGFVVDGEGKKMSKSVGNTIEAEEVIDK
                     YGAEILRLWVAAEDYTDDIRISDEILKRLVEAYRRIRNTSRFILGNLYDFDVNSAMVE
                     YAKMEEMDKWALHRLQEVIKRVTEAYERHQFHVVFYTIYNYCTVDLSALYLDVLKDRL
                     YTTRAKSSARRSGQTAMFTILKVMMQFLAPILTFTAEEVWAALPSWKGKEVSIHMTQF
                     PEVNAKYVNAEIGERWKAMIDAKAEIAKAIEQARKEKVIGHSLDARISIAAPGKMHNL
                     FKEHLEDLRALLIVSQLQLVDEKEISNPYKSEEIKGLTVGVAKARGVKCERCWIYSES
                     VGADAQHPTACDRCLTNL"
BASE COUNT          755 a          697 c          550 g          762 t
ORIGIN      
        1 gaatcgtgtc gaataatccg gttttgacca gtttaaccgc catctgcaga ttgtgcgaag
       61 taacacctaa aaatctaatt ttgccttctt tctttgcttt atcggctgca ctcaaagctc
      121 cattgtcttt tgtaattttc ccccattcct catcattggc cacctgatga aattgataca
      181 catcaatata gtcagtcttt aacattttga ggcttcgttc gagatgctta cgaaatcctg
      241 ccgcgtcacg cagggttgtt tttgtcgcaa taactatttt atcgcggaaa gaggatagag
      301 ccaggccgat tttgctttcg ctatcacggt acatatttgc agtatcataa aacgtgattc
      361 ccttatcgta ggcatggagc aaaactttta cagcttcatc ggtctgcagc cggattatcg
      421 gaatcccgcc aaaaccaact tcggacactt taagccctgt tttgcctaaa ctacgatagt
      481 ccatatatcc ttaccttgac aagcttgttt ttctccggtt caccagcatt ttccagatca
      541 ttaacacggc tcccactgaa atagccgaat cagccacgtt aaatgccggc cagtgccatg
      601 taccgatata aaaatcgaga aaatccacaa ccgcgccaaa gcgtatgcgg tcaataagat
      661 ttcccacagc cccgccaaag attaaagcaa gggatataac tataagcaaa gtttcctgct
      721 tacttttcat aatgtaataa atgatcagca aaatagccaa aacagtgatc ccaataaaga
      781 aaagataacg aaatatttcc ggcgctccgg ccaggaaacc aaaagccgca ccgggatttg
      841 tcacgtaaac aaggttaaaa aaaccatcaa taaccttgtg tgatccatac ataaataact
      901 tctcagtaat agctgctttc gttatttgat ccaatgcaat gactgcaata gcgccaataa
      961 taaaaataat taaatttctc ttcacagaaa atctcctcag tgtaaattta caagttagtt
     1021 aagcagcggt cgcaagcggt tggatgctgt gcgtcagcgc ctacggattc agaataaatc
     1081 cagcagcgtt cgcatttaac gccgcgcgct ttggccacgc caacagttaa tcctttaatt
     1141 tcttcgctct tgtagggatt actgatttct ttttcatcca ccagttgcaa ttgagaaacg
     1201 atcaacaagg cgcgtaaatc ttccaaatgt tctttaaaca agttgtgcat tttccccggc
     1261 gccgcaatgc taatgcgggc atccaaggaa tgaccgatta ctttttcttt tctggcctgc
     1321 tctatagctt tggcaatttc cgccttggca tcaatcatcg ccttccagcg ttcccctatt
     1381 tcggcattga catacttagc gtttacttcc ggaaactgcg tcatatggat actgacttct
     1441 ttgcctttcc aggaaggcag agccgcccat acttcttccg cggtaaaggt aaggatcgga
     1501 gctaaaaact gcatcatcac ctttaaaatt gtgaacattg ccgtctggcc ggagcgccgc
     1561 gccgaagatt tagccctggt tgtgtagagc ctgtctttta aaacatccag atacagagcg
     1621 ctcaaatcca ccgtgcaata gttgtaaatg gtatagaaaa cgacatggaa ttgatgccgc
     1681 tcataagctt ctgttacgcg cttgatcact tcctgcaagc gatgcaacgc ccacttatcc
     1741 atttcctcca ttttagcata ttcgaccatt gcggagttaa catcaaaatc ataaaggttg
     1801 cccagtatga aacggctggt attacgaatc cggcggtagg cttctactaa gcgtttaaga
     1861 atctcatcgg aaattcgaat atcgtcagtg taatcttcgg ccgccaccca gagccgcagg
     1921 atttccgctc cgtacttatc aataacttct tcagcttcaa tggtattgcc cacggacttg
     1981 gacattttct tgccttcgcc gtccacgaca aatccatgcg tgagaacgct caggtatggc
     2041 gcgcgtcctc ttgtccctac ggattccaaa agcgaagaat ggaaccagcc gcgatgctga
     2101 tcggagcctt ccagatacat atccgcgggc gagtgtaaat cttttctcgt ctccaaaaca
     2161 gccgcgtggg tcactcccga atcaaaccac acatccagga tatttgtttc tttgataaat
     2221 tcagaacttt tacaatgcgg gcaggttgtg cctttgggca tcaaatcctg ggcttccttt
     2281 tcaaaccaga tatcggcacc accagcctcg actaatttca ccaggtaatc gagcattttt
     2341 ttagtaagca cctcagcttt gcacttgcca caatagaata ctgtaatagg aacaccccac
     2401 aatctctggc gcgagataca ccaatcggga cggttttcca ccatgccgta aatgcggtca
     2461 tgcccccacg aaggaatcca ctcaacctga tcgatgcagg ccagagcttt tttgcgcaaa
     2521 tcgtttttct ccatggagat aaaccactgt tcggtggaac ggaagatgat gggattcttg
     2581 cagcgccagc aatgcggata ggaatgctcc aggggaaagt gtccgagcaa tgcgccaact
     2641 tcttttaatt ttttgatgac attatcattg gcatcgaaga caaactggcc ggcaaagtgt
     2701 tcgacatcgt cggtgaattt tccgttatca tccaccggag cgtaattttc caaaccgtat
     2761 ttaa
//
LOCUS       DASVIM010000028         8772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_693451, whole genome shotgun sequence.
ACCESSION   DASVIM010000028 DASVIM010000000
VERSION     DASVIM010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693451"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VF305_00520"
     CDS             complement(<1..422)
                     /locus_tag="VF305_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7415652.1"
                     /translation="MKISIASPEKILFWSHGEVKKPETINYRTFKPERDGLFCAKIFG
                     PVKDYECLCGRYKRMKHRGVVCEKCGVEVIQSKVRRERMGHITLATPVAHIWFLKSLP
                     SRIGNVMDLSLKELEKVLYFESWIVLDPKTTPLKKKELL"
     gene            complement(553..4689)
                     /gene="rpoB"
                     /locus_tag="VF305_00525"
     CDS             complement(553..4689)
                     /gene="rpoB"
                     /locus_tag="VF305_00525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7415653.1"
                     /translation="MGDFRKYFGRVKKVLDSPNLIEIQTRSYEKFLQKDLPPVKRTNI
                     GLQGAFKSVFPISDFSGKCSLEFVSYKIGDVRYDVKECIQKGMTYAAPLKIVVRLVVF
                     DTDRLADGKINRDAAEAKQIRDIKEQEIYFGEIPLMTENGTFIVNGTERVIVSQLHRS
                     PGIFFDHDKGKTVASGKLIYSARIIPIRGSWLDLEFDSKDLLYVRIDRRRKMPVTILL
                     KAMGNSTEFILNYFYNIQKAVIEGEKLYFTVDDSLAGQKAPEDIKDPKSAEVIVKKGR
                     KLTKPLLKRISDAGIKRVAIRETDLTGKILSSDIRDQKTGEIIFRCNEELPLNGLEIA
                     REKNIKELKFIYLDEDLDNASIRDTLLMDKIDTTEDAILEIYRRLRPSNPPTPETAAK
                     FFKSLFFEQETYDLSDVGRAKMNYKLHLNVPTDVTVLRNDDILSAVKYLIDLKNGDGE
                     CSVDDIDHLGNRRVRSVGELIENQYRIGLVRMERAIKEKMSLQDIETMMPHDLINAKP
                     VSAVVNEFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHY
                     GRICPVETPEGPNIGLIVSLSTYARVNEFGFIETPYRIVENGRVLDEVRFVTAIEEEN
                     QIIAPADRPLDKHGKFVEELISVRKGGDFISVMPEEIKMMDVSPNQLVSVAAALIPFL
                     EHDDANRALMGSNMQRQAVPLMCPEMPIVGTGMESVVARDSGAVLVAKRAGLVESVDA
                     SRIIVRADRPEEDSIDSGVDIYPLAKYQRSNQDTCFNQKPIVDIGDRLHKKDILADGP
                     ATDNGELALGRNVMVAFMSWGGYNYEDSILVSERIVKEDIYTSIHIEEFETMARDTKL
                     GKEEITRDIPNVGEEALRNLDESGIIRIGVSVKPGDILVGKITPKGETQLSAEEKLLR
                     AIFGEKASDVRDTSLRVPPGVEGTVIDAKIFTRKGADRDLRSQFIESEAIEQITKDRD
                     DEIHILTETIKKDITKLVVGKTAATKIVDAKKKTILKKGDKITKEGWANLPFTYWKEI
                     TITENDGTEEKLNNLFASLERKIDFVHAHFAEKLEKVKASDELPPGVIKMVKVYIAIK
                     RKLSVGDKMAGRHGNKGVLSRILPEEDMPYFADGSTVDIVLNPLGVPSRMNVGQILET
                     HLGWAAKSIGESLNELWEKNSNLNNIKNELKKTYSSPDFDKFIDQASGEDIKRFVGRL
                     KKGMLVASPVFDGCPENQIREMLKKADLPEAGQAILFDGRTGEPFDQEITVGIIYMMK
                     LHHLVDNKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWAMEAYGSAYTLQEFLT
                     VKSDDVAGRTRIYESIVKGEHTFEPGLPESFNVLVKELQSLGLDVELVEEADDVSQQR
                     "
     gene            complement(4726..5112)
                     /gene="rplL"
                     /locus_tag="VF305_00530"
     CDS             complement(4726..5112)
                     /gene="rplL"
                     /locus_tag="VF305_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX7415654.1"
                     /translation="MSSKITKDEVVKFIEEMTVLELSALVKELEEKFGVSAAAPMMAA
                     VAVGGGAPAAAAEEQTEFNAILTGFGAEKIQVIKVVRAITGLGLKEAKDLVEGVPKPV
                     KEGVSKDEAADIKKKIEEVGGTIEIK"
     gene            complement(5140..5664)
                     /gene="rplJ"
                     /locus_tag="VF305_00535"
     CDS             complement(5140..5664)
                     /gene="rplJ"
                     /locus_tag="VF305_00535"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX7415655.1"
                     /translation="MDRRTKEQIVSELQEKLKEAKLVVLTSFSGMNVEKMEALRNALR
                     KSNAELKVVKNTLLGIASKETEFSVLADHFQWPIAVVLSYKDPVEPTKVLVDFAKKNP
                     ELEIKVGVLDGKLLTKGDLNILAELPGREVLLGKLVLVMAAVPTSLVTVLSGVPRNFV
                     QVLNAYCDKKKTLN"
     gene            complement(5907..6605)
                     /gene="rplA"
                     /locus_tag="VF305_00540"
     CDS             complement(5907..6605)
                     /gene="rplA"
                     /locus_tag="VF305_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX7415656.1"
                     /translation="MLRRGKRILAAKAKVEPTKRYTLKEATEMIVSMPGAKFDETVDA
                     AVRLGVNPAHADQMVRGSVVLPNGLGKSVRVLVFAKGDKEKEALEAGADLVGNDDVIE
                     KIKGGWLEFDRVIATPDMMGSVGKIGKILGPRGLMPNPKVGTVTFEVANAVKELKAGK
                     VEFRVEKAGIIHSPVGKVSFGAEKLRENVSALLETIIKLKPASSKGIYIKSISLSSTM
                     GAGVRIDSLDIKTV"
     gene            complement(6624..7049)
                     /gene="rplK"
                     /locus_tag="VF305_00545"
     CDS             complement(6624..7049)
                     /gene="rplK"
                     /locus_tag="VF305_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX7415657.1"
                     /translation="MAKKVIANIKLQIKAGKATPSPPIGPALGQHGVNIMEFCKAYNA
                     LTQNQEGMVIPVVITVYADRSFTFVTKTPPASILLKQAAKIAKGASDPKREKVGTVTA
                     NQVQEIAQLKYKDLNAVNIEGAIKIIAGTARSMGIEIAG"
     gene            complement(7106..7636)
                     /gene="nusG"
                     /locus_tag="VF305_00550"
     CDS             complement(7106..7636)
                     /gene="nusG"
                     /locus_tag="VF305_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX7415658.1"
                     /translation="MAFKWYVVHTYSGYENKVKTSLQERIEMAGAQAYFSDILIPEED
                     VVELISGEKKTSKRKFFPGYILVRMEMSDETWHIVKDTPKVTGFIGSKDKPSPIPDKD
                     VENLKVRIDEGTLKPKPKFKFDVGDHVKIIDGPFTNFDGVIDEVKPEKSKLRVIVSIF
                     SRPTPVELDFIQVTQG"
     gene            complement(7641..7844)
                     /gene="secE"
                     /locus_tag="VF305_00555"
     CDS             complement(7641..7844)
                     /gene="secE"
                     /locus_tag="VF305_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX7415659.1"
                     /translation="MQKIKIILQKIIQFLNEAKAELKKVTWPSPKQTLASTAVVIIIV
                     FIIAIYLSIIDYGLAKLVKFILG"
     gene            complement(7874..7950)
                     /locus_tag="VF305_00560"
     tRNA            complement(7874..7950)
                     /locus_tag="VF305_00560"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7914..7916),aa:Trp,seq:cca)
     gene            complement(7964..8113)
                     /gene="rpmG"
                     /locus_tag="VF305_00565"
     CDS             complement(7964..8113)
                     /gene="rpmG"
                     /locus_tag="VF305_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX7415660.1"
                     /translation="MRSNIILACTECKQRNYTTTKNKRTIQNRLEMKKYCKFCRGHKL
                     HRETK"
     gene            complement(8127..8202)
                     /locus_tag="VF305_00570"
     tRNA            complement(8127..8202)
                     /locus_tag="VF305_00570"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8167..8169),aa:Thr,seq:ggt)
     gene            complement(8242..8317)
                     /locus_tag="VF305_00575"
     tRNA            complement(8242..8317)
                     /locus_tag="VF305_00575"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8282..8284),aa:Gly,seq:tcc)
     gene            complement(8341..8425)
                     /locus_tag="VF305_00580"
     tRNA            complement(8341..8425)
                     /locus_tag="VF305_00580"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8389..8391),aa:Tyr,seq:gta)
     gene            complement(8444..8519)
                     /locus_tag="VF305_00585"
     tRNA            complement(8444..8519)
                     /locus_tag="VF305_00585"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8484..8486),aa:Thr,seq:tgt)
BASE COUNT         2321 a         2186 c         1586 g         2679 t
ORIGIN      
        1 agcagctctt ttttcttcaa aggagtagtt ttagggtcga ggacgatcca agattcaaaa
       61 taaagaacct tttccagttc cttcaaggat aaatccatga cgttaccgat acggctggga
      121 agactcttta aaaaccaaat atgggccacg ggagttgcca gtgtaatatg tcccattctt
      181 tcccggcgca ctttagattg aataacttct acaccgcact tttcacaaac tactccacgg
      241 tgtttcattc tcttataacg cccgcaaaga cattcatagt cctttaccgg cccaaaaatc
      301 ttcgcgcaaa aaagcccatc tctttccggt ttaaatgtcc ggtagttgat tgtttccggt
      361 ttctttactt cgccatgtga ccaaaaaaga atcttttccg gcgaagcaat agaaatcttc
      421 attgcattaa atctaatcgg attttttggt ttttcaaaat aactaaaaac gtcttccaca
      481 gattttctcc tgtactatta aatagcttaa gtgaaatttc tgagttactt atgccaagtc
      541 atcttcaatt ggctaccttt gttgactcac gtcgtcggct tcctcaacca attccacgtc
      601 taatccgagg ctttggagtt ctttaacaag aacgttaaag gattccggca gacccggctc
      661 aaatgtatgc tcacctttga cgatagattc gtaaatcctt gttcgtcccg ctacatcatc
      721 ggatttgacc gtaagaaatt cttgcaacgt ataagcagat ccataagctt ccatcgccca
      781 cacttccatt tcacccaatc tctggccgcc aaattgggct ttaccgccca acggctgttg
      841 ggtaaccagc gaataggggc caatggaacg agcatgaatt ttattgtcaa ccagatggtg
      901 cagtttcatc atgtaaatta tgccgaccgt aatttcctga tcgaatggct cgccagttct
      961 accgtcaaag agaattgctt gtcctgcttc cggcaaatca gcttttttta acatttctct
     1021 aatttgattt tcgggacaac cgtcaaaaac cgggctcgct actaacattc cttttttcaa
     1081 acgccccaca aatcttttga tatcttcgcc ggaagcttga tcaataaatt tgtcaaaatc
     1141 cggggaagaa tatgtttttt tcaattcgtt tttgatatta ttcaaattag aattcttttc
     1201 ccacagttca ttcaagcttt cgccgattga ttttgccgcc cagcctagat gggtctccag
     1261 aatttgccca acattcatcc gtgaaggaac gcctaaggga ttcaagacaa tatccacggt
     1321 agaaccgtca gcaaaataag gcatatcttc ttccggcaaa atacgcgaca aaactccttt
     1381 attgccatgc cttcccgcca tcttgtcacc tacggataat tttctcttga tggctatata
     1441 aactttgacc attttaataa caccgggagg aagctcatcg ctggctttaa ccttctccag
     1501 tttctctgca aaatgggcat ggacaaaatc aatttttctc tccaaagaag caaacaagtt
     1561 atttaacttt tcttctgtgc catcgttttc agtaatagta atttctttcc agtaagtaaa
     1621 aggcagatta gcccagcctt ctttggtgat tttatcgcct ttttttaaaa tcgttttctt
     1681 tttagcatcc actatcttgg ttgctgcagt ttttccgaca acaagctttg taatatcttt
     1741 tttaattgtt tcagtcagga tatgaatttc atcgtctcga tctttggtta tttgctcaat
     1801 agcttcactt tcgataaact gcgaacgcaa gtccctatcc gcgcctttcc gtgtgaatat
     1861 ttttgcgtca ataaccgtac cttcaactcc aggaggcacg cgtaaagatg tatcacgcac
     1921 atcgctcgcc ttttccccga atattgcccg caggagtttc tcttcggcag aaagctgggt
     1981 ctctcctttg ggagtgatct tgccgacaag gatatctcct ggtttgaccg aaacgccaat
     2041 gcgaataatg ccgctttcat ctaaatttct cagcgcttct tcaccgacat tgggaatgtc
     2101 ccgcgtaatt tcttcttttc ccaatttagt atcgcgagcc atcgtttcaa attcttcgat
     2161 atgaattgac gtgtaaatgt cttctttaac tattctttcg ctgactaaaa tagagtcttc
     2221 atagttgtaa ccaccccagg acatgaaggc aaccataaca tttctgccga gtgccaactc
     2281 gccgttatca gttgccggcc catcagccaa tatatctttt ttatggaggc gatcaccaat
     2341 atcaactatc ggtttctggt taaaacaagt atcctggtta gagcgctggt atttagctaa
     2401 aggataaata tccactccgc tatcgatgct atcttcttcg ggacggtcag ctcgaacaat
     2461 aatccgcgag gcatcaacgc tttccacaag ccctgctctc ttggccacga gaactgcccc
     2521 ggaatcacgg gcgacaaccg attccatacc cgtacccact atcggcattt ccggacacat
     2581 cagcggtaca gcttgcctct gcatgtttga ccccataagt gcacggttgg catcatcatg
     2641 ttccaaaaac ggaattaatg ccgccgctac actgacgagt tggttaggcg aaacatccat
     2701 cattttaatt tcttccggca taacggaaat aaaatcgccc ccttttctaa cggatatcag
     2761 ctcctcaaca aacttgccgt gtttatccaa gggacgatca gccggtgcaa tgatctggtt
     2821 ttcttcttca atcgccgtca caaaacggac ttcatctaac acgcggccat tttctactat
     2881 cctataaggt gtttcaataa agccaaattc gttaacccga gcataagtgc tcaaagacac
     2941 aatcaaacca atatttggtc cttccggtgt ctctaccgga caaatacgac catagtgggt
     3001 gggatggacg tcgcggactt caaatccggc cctttcgcgc gtcaaaccgc ccggccctaa
     3061 tgcggataaa cgtcttttgt gcgtaatttc agaaagggga ttagtctgat ccataaactg
     3121 agaaagctgg ctggaaccaa aaaactcatt aacaacggca gaaacaggct tagcgttaat
     3181 caagtcatgc ggcatcattg tttcgatatc ctgcaagctc attttttctt taatcgcccg
     3241 ctccatgcgc accaaaccaa tacggtactg gttttcaata agctcgccga cagatcgcac
     3301 cctccggttc cccaagtgat cgatgtcatc gacactacac tcaccgtcac catttttcaa
     3361 atcgatcaaa tatttaacag cagataagat atcatcatta cgcaatacag taacgtcagt
     3421 cggcacattc agatgtaatt tgtaattcat tttggcgcgg ccgacatccg atagatcgta
     3481 agtttcctgt tcaaaaaata ggcttttaaa aaacttggct gccgtttccg gcgtgggagg
     3541 attacttggg cgcaatcggc gatatatttc caatatcgcg tcttcagttg tatcgatttt
     3601 atccatcaat agcgtatcgc gaatggaggc attatccaga tcttcatcga gatagatgaa
     3661 ctttaactct ttgatattct tctctctggc tatttccaaa ccatttagcg gtaattcttc
     3721 attgcaacga aagataatct ctcctgtttt ttgatcacga atatccgaag aaagaatctt
     3781 accggtaaga tcagtttccc ttattgcaac acgcttaatg ccggcatccg atatcctctt
     3841 taaaagaggc ttggtcaact tgcgcccttt tttaactatc acttcagcac ttttgggatc
     3901 tttgatgtct tcgggcgctt tttgaccggc aagtgaatca tcaacggtaa agtaaagctt
     3961 ttcaccttcg ataacagctt tttgaatgtt gtaaaaataa tttaaaataa attcagtcga
     4021 attgcccatc gccttgagca aaatagtcac cggcattttg cggcggcggt cgatccggac
     4081 atacagaagg tccttagaat caaattccaa atccagccag gaacctctga tgggaataat
     4141 tcgcgctgaa taaattaact tgccgctggc gacagtttta cccttgtcat gatcaaaaaa
     4201 gatgccggga gatcggtgca gctggctgac aatgaccctc tccgttccgt taacaatgaa
     4261 ggtaccgttt tcagtcatca gcggaatctc gccgaaataa atttcctgct ccttgatgtc
     4321 gcgaatctgc ttcgcttccg ctgcatcccg gttgatcttg ccgtcagcaa ggcggtcagt
     4381 atcaaataca accagcctga ccacaatttt caaaggagcg gcataggtca tgcccttttg
     4441 gatacactcc tttacatcat accggacatc gccaatttta tagctaacga attccagaga
     4501 acactttccg ctaaaatcag aaatggggaa aacgctcttg aaagccccct gcaaacctat
     4561 attagtccgc ttcactggcg gcaggtcctt ttggaggaac ttttcgtaag atcgtgtctg
     4621 gatttcaatt aaattgggac tatccagtac tttttttacc cggccaaaat attttctaaa
     4681 atcgcccata gcatccttca ataaaataat atattagaga ttaaatcatt taatctcgat
     4741 agttccacca acttcttcaa ttttcttctt aatatcggca gcttcatctt tggatacgcc
     4801 ttctttaaca ggtttgggta caccttccac caaatctttt gcttccttca gccctaagcc
     4861 ggtaattgcc cgaacaactt taattacctg aatcttttct gcaccaaaac cggttaaaat
     4921 tgcattaaat tcagtttgtt cttctgccgc agccgccgga gcgccacccc cgaccgcaac
     4981 tgccgccatc attggagccg ccgcggaaac accaaacttc tcttccaatt ctttcaccag
     5041 agcagaaagc tctaataccg tcatttcttc aataaatttg accacttcgt ctttagtaat
     5101 tttacttgac attttttcta ttcctctctg tatgttttat tagtttaaag tttttttctt
     5161 gtcacagtag gcattgagca cttgcacaaa gtttctcgga acgccactca acaccgtcac
     5221 aagcgatgtt ggtaccgcgg ccatcaccaa aactaacttg cctaaaagaa cctctctgcc
     5281 aggtaattcc gccaagatgt taagatcgcc cttggttaaa agcttgccat ccaatacgcc
     5341 gactttgatt tccaattccg gatttttctt ggcaaaatca acgagaactt ttgtcggttc
     5401 caccggatct ttatagctca aaacaacggc aatgggccat tggaaatggt cggcaagaac
     5461 actgaattcg gtttcttttg aggcaattcc caaaagcgtg ttttttacaa ccttcaattc
     5521 agcattggac ttgcgcagcg cattccttaa agcttccatc ttttcgacgt tcatcccact
     5581 gaaactggtt aaaacgacta gcttggcttc tttgagtttc tcctgcagtt cggatactat
     5641 ttgctctttt gttctccgat ccaatttatc agcctccttt caaaaattaa tttatttgcc
     5701 tgaggccgga gaatatagcc acatacagaa caaaacggta aaccttgcct aactccgatt
     5761 cccgttttcc aaaaatcgtt taccttaaaa aaataaagta acctttttta ttccacgagt
     5821 ctcggcaggc cgctttacgt ttaaacttct gtacctgctg tcttcgacct ttttattata
     5881 attataaaac gcttaatctc tctgccttaa acggtcttaa tatcgagaga atcaattctc
     5941 acacccgcac ccattgtgct ggagagggaa atactcttaa tataaattcc cttactggac
     6001 gccggtttta atttgataat tgtttccaat aaagcgctaa cgttttccct caatttttcg
     6061 gctccaaaag aaactttgcc caccggagag tggatgattc cagctttttc cacgcggaat
     6121 tcgactttgc ctgctttgag ttccttcact gcattggcca cttcaaaagt taccgttccc
     6181 actttcggat tgggcatcaa accgcgcggg cctaaaatct taccaatctt tcccacggaa
     6241 cccatcatat ccggcgtggc aataacacga tcaaattcca gccagccccc tttaattttt
     6301 tcaataacat cgtcattgcc gactaaatct gcaccggctt ccagagcttc tttttctttg
     6361 tcgcccttgg caaatactaa aacgcgcact gacttaccca agccattagg caagacaaca
     6421 ctgccccgaa ccatttggtc ggcatgagca ggattaacgc ctaaacgaac agcggcatcc
     6481 accgtttcgt caaatttggc accaggcatc gatacaatca tttcagtagc ctctttaagc
     6541 gtatagcgct tggtcggctc aacttttgct ttcgctgcta atatgcgttt gcctcttctt
     6601 aacatgataa aacctcgtct ttattaacct gcaatctcaa ttcccattga cctggccgta
     6661 ccggcaatta tctttatagc gccttcaatg ttcacggcat ttaaatcttt atattttaac
     6721 tgcgcaattt cctgcacttg attggccgta actgtcccaa ctttttccct cttcggatcg
     6781 ctagctcctt tagcaatttt ggccgcttgc ttgagcagta tagacgccgg cggtgttttg
     6841 gttacaaatg taaacgacct atctgcataa acggtaatca ctacaggaat aaccattcct
     6901 tcttggtttt gcgtcagagc attatacgct ttgcaaaatt ccataatgtt gacaccatgc
     6961 tgtcctaatg caggaccgat cggcggcgaa ggagtcgcct tgcccgcctt tatttgtaat
     7021 tttatattcg caataacttt ttttgccatg ttgatcaccc tgatctaaaa atttttaaag
     7081 tttcgttaat ctgccgcgta aaatcttaac cttgcgttac ctgaatgaag tctaattcca
     7141 ccggtgtcgg ccggctaaaa atgctcacga taactcttaa tttacttttt tccggcttta
     7201 cttcatcaat aactccgtca aaattggtaa acggcccatc gataattttt acatggtcac
     7261 caacatcgaa tttgaacttg ggcttgggct ttaaagttcc ttcatcaatc ctgaccttta
     7321 aattttctac atctttatcc ggtatgggcg aaggcttatc tttactgccg atgaagccgg
     7381 taactttggg ggtgtctttt acaatgtgcc aagtttcatc actcatttcc atgcggacaa
     7441 gaatgtatcc cgggaaaaac ttacgtttgg aagtcttctt ttcccctgaa attaattcca
     7501 caacatcttc ctcgggaata aggatgtcgg aaaaataagc ttgcgctcca gccatctcaa
     7561 ttctttcctg gaggctcgtt tttaccttgt tctcgtatcc ggaatatgta tgaacaacgt
     7621 accatttaaa agccattttg ttaacctaat ataaatttaa ccagtttagc aagaccgtaa
     7681 tcaattatac tcagataaat cgctatgata aaaacgataa ttattacaac cgccgtcgaa
     7741 gccaacgtct gcttcgggct cggccaagta actttcttaa gctccgcctt ggcctcgttg
     7801 agaaactgga ttatcttttg caatattatt tttatttttt gcatactttt taaatcctta
     7861 cttaagaaaa aaatggcagg ccaggaggga cttgaacccc cagcatccgg atttggagtc
     7921 cggcgctcta tccattagag ctactggcct ataccaatat caactacttg gtttctctgt
     7981 gcagcttatg cccccggcaa aatttgcagt attttttcat ttccaagcgg ttttggatgg
     8041 tacgcttatt ttttgtggtc gtgtaattcc tttgcttgca ttccgtacaa gccaatataa
     8101 tattactcct cataacactc cttaactgga gcccacgacc agaattgaac tggtgacctc
     8161 atccttacca aggatgtgct ctaccaactg agctacgtgg gcacaatctc ttaattcttt
     8221 ttgcccttat tttaaaatac ttggagcggg aaacgggatt cgaacccgcg accctcagct
     8281 tggaaggctg acgctctagc cactgagcta ctcccgctta ttactaatat caaaaatcct
     8341 tggtggaggg gggagggttc gaacctccgt aggcttcgcc ggcagattta cagtctgctc
     8401 cctttggccg ctcgggaacc cctccagtta aacattacca aactggagct ggcgatggga
     8461 ctcgaacccg caacctgctg attacaaatc agctgctcta ccgattgagc tacgccagca
     8521 aatcccacct taattatctt ttaatattaa gctcttatgc tttaaagcac aaaaaaacac
     8581 aactacgccc ttaagcgaat cgccgaatct aatttttata gatgtttttg tcaagtaaat
     8641 ttttaaaaaa tcttatagat aggcgtgcaa tcttgttcga ctgcgtgaat gcgcggacga
     8701 tcttttctgc acacttcaat tatggaatac ttttttttgg taaggtcgaa cgacccaagg
     8761 taaccacaat gt
//
LOCUS       DASVIM010000029         3925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1035488, whole genome shotgun sequence.
ACCESSION   DASVIM010000029 DASVIM010000000
VERSION     DASVIM010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035488"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VF305_00590"
     CDS             complement(<1..506)
                     /locus_tag="VF305_00590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEX7415661.1"
                     /translation="MTARKILHEATRDFEAVGIPSARLDAEVLLSFCLGRDRLDFLKN
                     PEMPISEIHASLFKKIIARRLLWEPVAYITGRKEFWSFTLEVNNSVLIPRPDTEILVE
                     EALNICRKINSSEINILDMGTGSGAIALALAKEIPHAQLVATDISSAALAVARKNACN
                     LGLKNQID"
     gene            complement(547..1626)
                     /gene="prfA"
                     /locus_tag="VF305_00595"
     CDS             complement(547..1626)
                     /gene="prfA"
                     /locus_tag="VF305_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX7415662.1"
                     /translation="MDVILDKLRDIEIHYRELEGQLSDPQVVSKQGLYQKYAKEHADL
                     RELVEIIRQYEKIRTRIAEYHGFLKESDEELKAIVKEEMPQLQQEQAELEEKIKNLLL
                     PKDPNDNKNVFLEIRAGTGGDEAGLFVGDLFRMYARYAEAQGWRVEVVSSSPAGGVGG
                     FKEIIAQIDGKGAYSRLKYESGVHRVQRVPVTEAQGRIHTSAVTVAIMPEAEEVELNI
                     DPNELRIDVYRSTGHGGQSVNTTDSAVRITHLPTGLVVTCQDEKSQLKNKVKAMKVLR
                     ARLLDAMVQKQNAEISEVRKSQVGTGDRSERIRTYNFPQGRITDHRISLTRYDLDNFL
                     NGNIGAMIDALATHYQAEALNKPGN"
     gene            complement(1785..2021)
                     /gene="rpmE"
                     /locus_tag="VF305_00600"
     CDS             complement(1785..2021)
                     /gene="rpmE"
                     /locus_tag="VF305_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX7415663.1"
                     /translation="MKEGIHPEYKETTITCVCGNVLKTRSTKKDIKIEICSNCHPFIT
                     GKQKIVDTAGRVERFKRRYEPKAEKPQTKKKTTT"
     gene            complement(2348..3595)
                     /gene="rho"
                     /locus_tag="VF305_00605"
     CDS             complement(2348..3595)
                     /gene="rho"
                     /locus_tag="VF305_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX7415664.1"
                     /translation="MNIEDIKRLPISELNNLAKDLDVPGASGMRRQDLIFAILQTQAE
                     QNGVISGSGVLEILPDGFGFLRAVDYNYLPSPDDIYISPSQIRRFNLKTGDTISGEVR
                     PPKEGEKYFALLKVDTVNFESPEQARDKILFDNLTPLYPLEKLNLEFDPENFSTRTLD
                     LFAPIGKGQRALIVSPPRAGKTVLLQDIAHSITANHKEVVLMVLLIDERPEEVTDMQR
                     SVAGEVISSTFDEPASRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVP
                     PSGKVLSGGVDSNALHKPKRFFGAARNIENGGSLTIISTALIDTGSRMDEVIFEEFKG
                     TGNMELHLDRRIADRRVFPAFDLIRSGTRKEELLIPKANLNRIWILRRLLQEMNPVDA
                     MEFIIGKIKKTETNQEFLDSMNS"
BASE COUNT          991 a          890 c          845 g         1199 t
ORIGIN      
        1 aaatcaattt gatttttcag ccccaaatta caggcgttct tcctggctac ggcaagagcg
       61 gcagaggaaa tgtcggtggc cactaattga gcatgaggta tttctttggc taaagcaagg
      121 gctatcgcac cgctgcccgt gcccatatct agaatattta tttcggaaga attaattttg
      181 cggcaaatgt tgagggcttc ttccaccagg atttctgtat ctggccgggg aatcaatacg
      241 gagttattta cttccagcgt aaaagaccaa aattctttgc gcccggtaat ataggccacc
      301 ggttcccata gcaatcttcg ggcgatgatt tttttaaata aagaagcatg aatctcgcta
      361 atgggcattt ccggattttt gagaaaatcg agccggtctc gtcctaagca aaaagaaagt
      421 aatacctcag catccaatcg cgccgaagga attcccacgg cttcgaaatc ccgggttgcc
      481 tcatggagaa ttttccgagc agtcatactt ctgattcgcg tagagagcta atgtttcaga
      541 tggatattaa tttcctggtt tatttaacgc ttctgcctgg taatgtgtcg ccaatgcgtc
      601 gatcatagcg ccgatattgc cgttgaggaa attatcgaga tcataacgag taaggctgat
      661 acgatgatcg gttattcgcc cttgggggaa gttatatgtg cggattcgtt cgctgcgatc
      721 accggtgccc acctgacttt tacgtacttc cgatatttcc gcgttttgtt tttgtaccat
      781 ggcgtctaac aggcgtgctc gcaatacttt cattgctttg accttgtttt taagctggga
      841 tttttcatcc tgacaggtta caacaagtcc cgtcggcaga tgggtaatgc gtacggcaga
      901 atccgttgta ttgacgcttt ggccgccgtg accggtggaa cgataaacat caatccgtaa
      961 ttcattggga tcgatgttga gctccacttc ttccgcctcc ggcataatgg caacggtaac
     1021 ggcggaggtg tgaatgcgtc cttgcgcttc cgtaaccgga acgcgctgca ctcggtggac
     1081 gccgctttcg tacttcaggc ggctataagc gcccttgccg tcaatttggg cgattatttc
     1141 cttgaatcca ccaacaccac cagccggaga gctgctcacc acttccaccc gccagccttg
     1201 tgcttccgcg tagcgcgcat acatgcgaaa taaatcgccc acaaaaaggc cggcttcatc
     1261 gcctcccgta cccgcccgaa tttccaaaaa gacgttttta ttatcgttgg ggtctttcgg
     1321 cagcagcaaa tttttgatct tttcttccaa ttcagcctgt tcttgctgca attgaggcat
     1381 ttcttccttg acgattgctt ttagttcttc gtcactttcc tttaaaaagc cgtgatactc
     1441 tgcaatacgg gttcgaattt tttcgtactg ccggataatt tccactaatt cccttaaatc
     1501 cgcatgctct ttggcgtatt tttgataaag accttgcttg gaaacaactt gagggtcgct
     1561 aagttgcccc tccagttccc tgtaatgtat ttcaatgtcg cgaagtttgt ctagtataac
     1621 atccatatta tatctttaaa aattttaaga tgtttaaaat tagttgaaaa aggcgcacat
     1681 ataggctcct tttcgctgga aaaggtacct acaaatggta aggatgatga acaatctttg
     1741 tcataagggc aggatccaat attttggctt ccaattgcga aaaattatgt ggttgttttc
     1801 tttttggttt gtggcttttc tgctttgggt tcgtatcttc tcttgaatct ctccacgcgg
     1861 cctgccgtat ccacaatctt ttgcttacct gttataaaag gatggcagtt ggaacaaatt
     1921 tctatcttaa tatctttttt agttgacctt gtcttgagga cattgccgca aacacaagtg
     1981 atggttgttt ctttatattc cggatgaatg ccttctttca tgtctttttg ttttcctcct
     2041 tagcatttat ctgcaactag taaaattcta gtataatttc agagcagtgt ggttgcctta
     2101 taatatgtgc aataaaaatt caagaaaata tttaacgact tcgtaaaaag tccccagcat
     2161 tcgccgggct gcgcatatgc ggagtatatc ccgcgcatgc cggaggcact gcatccttcg
     2221 tcactgcggc ctacgtagaa gtaggcctca ttcctcaggc tttgtgcgcc ttgcctctgg
     2281 cgcattttac aaagtcgtcc cagaattggc aatttgcgtc tttttacgaa agcatcatat
     2341 ttggttctta tgaattcatc gaatccaaaa attcttgatt agtttccgtt ttcttaattt
     2401 tgccgataat aaattccatg gcatcaacgg gattcatttc ctgtagcagt ctgcgtaaaa
     2461 tccagatacg gttgagattc gccttcggaa tcagcaattc ttcttttctt gttcccgaac
     2521 ggatcaaatc aaacgcgggg aagactctgc ggtcggcaat gcggcggtcc aaatgcaatt
     2581 ccatattgcc tgttcccttg aattcttcga aaatgacttc gtccatccgg cttccggtat
     2641 caatcaaagc ggtagaaatg atcgtcagac tgccgccatt ttcgatattt ctggccgcgc
     2701 cgaaaaaacg cttcggctta tgcagagcgt tggaatcgac accgccggaa agaacttttc
     2761 cgctgggcgg aacaacggta ttgtaagcgc gcgccaaacg ggtgatgctg tcgagcaaaa
     2821 tgaccacatc tcttttatgc tcgaccaggc gtttagcctt ttcaatgacc atttccgcga
     2881 cttgcacgtg acgggaagca ggttcgtcaa atgtggaaga aattacttca ccggcgacgc
     2941 tgcgctgcat gtcggttact tcttccggcc gttcgtcaat tagaagcacc attaaaacta
     3001 cttctttatg attggccgtg atgctatggg caatatcctg caaaaggact gttttaccgg
     3061 cacgcggcgg agaaacaatt aaagcacgct gtcccttgcc gatcggtgca aacaaatcca
     3121 atgttctggt ggaaaaattt tcgggatcaa attccagatt caatttttcc agaggataaa
     3181 gaggagtcag gttatcaaaa aggattttgt cgcgggcttg ttcggggctt tcaaaattta
     3241 cggtatcaac tttcaggagg gcaaaatatt tttccccttc tttgggcggc cggacttcgc
     3301 cggaaatggt gtcgcctgtt tttaaattaa accttctgat ttgcgagggt gaaatgtaaa
     3361 tatcatccgg gctgggcagg tagttgtaat ccacggcgcg taaaaaacca aaaccatccg
     3421 gcagaatttc caatactccc gagccggaaa ttacgccatt ttgttccgct tgcgtctgta
     3481 aaatggcgaa aatcaaatcc tgacgacgca tgccgctggc gcccggaaca tctaaatcct
     3541 tggctaagtt gttgagttcg ctaatcggca atcgcttaat gtcttcaatg ttcataaata
     3601 cttccttact ttaaattata atcgggcaac gagaaacgtt atcctgatac tatatatttg
     3661 cttttttact gcattatttt tcaagaaaca cacaaagata atcacgaatt ttttaaaagt
     3721 tacggattaa atatcggctt gtttatttga atacttgaaa cgctccaaag gacagggctt
     3781 aaaaaagcct cttaatgaat tgcgaagtac aatatattaa tctagagcga ctgtcaagat
     3841 attttattgt tttcaacaaa tttctcttgg aaaagaatgt ttttacgttt atgatcaatt
     3901 attccttgat tcctgcgcaa cacta
//
LOCUS       DASVIM010000030         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_438012, whole genome shotgun sequence.
ACCESSION   DASVIM010000030 DASVIM010000000
VERSION     DASVIM010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438012"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1169
                     /gene="rsmA"
                     /locus_tag="VF305_00610"
     CDS             315..1169
                     /gene="rsmA"
                     /locus_tag="VF305_00610"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX7415665.1"
                     /translation="MLTPKEIIHQYGIKPHKRLGQSFLLDGNVIKKIATVADISSEDI
                     VIEIGAGIGVLTEEITQIARKVIAVEIDTQLVEILKDKLGGYSNVEIHSGDILKYDFN
                     SIAQRYKTKVRVVGNVPYNISSPLLFHLLSSRSVINGFILMLKREVVQRLVSLPNNKS
                     YGVPSVILQMFASVEKIFDVPSTCFYPRPKVESAVIKGVFLDKPLMELADEAFFTQLV
                     KASFAQRRKMLMNNLKNAKLLDGIPEAKLKDALSSTGIDGKRRGETLSIEEFGKLSNL
                     LCRYKNHL"
     gene            1243..>2090
                     /gene="miaB"
                     /locus_tag="VF305_00615"
     CDS             1243..>2090
                     /gene="miaB"
                     /locus_tag="VF305_00615"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX7415666.1"
                     /translation="MKNKYLYIQTFGCQMNVHDSEQMAALLANIGYNKTDNKKIADLI
                     LLNTCSIREKAAQKFFSELGRLVKLKEENPALIIGVGGCLAQHLGHKIFKRVAQVDLV
                     FGTHNIHLLPELINTLQKEKTKITQIGFQKSLNSIGVFAPPEKGAVSAFVTIMQGCNN
                     FCAYCVVPYLRGPEISRSPEDIIEEIKKLVDCGIKEVTLLGQNVNSYGKTLANGLNFT
                     ALIKKIGKISGIKRIRFTTSHPRDLSEELINCFAEEGKLCDHIHLPVQSGSNRVLALM
                     NRGYTV"
BASE COUNT          650 a          382 c          457 g          601 t
ORIGIN      
        1 gcaatgattg cagttatcgg cgagattatg ctgaaaaacg gccgcagtga tccgctggat
       61 ttgaacgcga tatcgcgctg gcctctcgtt tcatatccat aaaattccct ctccttttga
      121 gggcatttta cccgatgctg cgcagcaggt ttgttatgga ggaaacaact gtactgtcat
      181 cgcgaggagt acgctaatgg cggacgacgt ggcgatctct aagggcatga gattgcttcg
      241 cttcactcgc aatgacccgt ttgagtttct cagaagctct agaccaatca atgctctgga
      301 atgatagtat atttatgctg acaccaaaag aaataattca tcaatatggt attaaaccgc
      361 acaagagatt aggtcagtca tttcttttgg atggtaatgt tattaagaaa atagccactg
      421 tggctgacat ttcgtcagaa gatatagtca tcgagatagg cgcgggtatt ggcgtattaa
      481 cagaggaaat tacccagata gccagaaaag tgattgctgt agaaatagat actcaattag
      541 tggaaatctt aaaagataaa cttggcggat atagtaatgt agaaattcat tccggagata
      601 tactgaaata tgatttcaat tccatagctc aaagatataa aactaaagtt agggttgtgg
      661 gtaatgttcc atacaatata tccagcccct tgctttttca tttattatct tccagatcag
      721 tcatcaatgg ctttatcctc atgctcaaaa gagaggttgt tcagcggttg gtgtctttgc
      781 ccaataataa aagctatggt gtgccttcgg taattttgca aatgtttgct tctgtcgaaa
      841 aaatatttga tgtgccctca acctgttttt atccccggcc aaaagtagaa tcggcggtga
      901 tcaagggtgt ttttctggat aagcctttaa tggaattagc tgatgaagct ttttttaccc
      961 aactggttaa ggcgtctttt gcccagcgcc ggaaaatgct gatgaacaat ttgaagaatg
     1021 caaaattatt agacgggatt ccggaagcga agttaaaaga tgcgttgtct tctaccggta
     1081 ttgacggaaa aagaagaggg gagactcttt ctattgagga atttggcaag ttaagcaatt
     1141 tgctttgtcg ttataaaaat catctataat ttaatcaaaa acctattgac taactttcgt
     1201 tgaaatatta aggctgtcca caaagccacc tggcgaaaaa acatgaaaaa taaatactta
     1261 tatattcaaa catttggttg tcaaatgaac gttcatgatt ccgaacaaat ggccgccttg
     1321 cttgctaata ttggatacaa taaaaccgat aacaaaaaaa tagcggattt aattctgctc
     1381 aatacctgtt cgattcgaga aaaagcagcg caaaaatttt tcagtgaatt aggcaggctg
     1441 gttaaattaa aagaggaaaa tccggcattg ataatcggcg ttggcggctg tttggcgcag
     1501 catttaggcc ataagatttt taaaagagtg gcgcaggttg atctggtttt cggcacccat
     1561 aacatccatc tattacccga gttaatcaac acattgcaaa aagaaaaaac aaagattacg
     1621 caaattggtt ttcagaaatc gctcaattcc ataggcgtat ttgctccacc ggaaaagggc
     1681 gcagtcagcg cctttgtcac aataatgcaa gggtgtaata atttttgtgc ctattgcgtt
     1741 gtcccttatt tgcgcgggcc ggaaataagc cggtcgccgg aagatatcat cgaagaaata
     1801 aaaaagctgg ttgattgcgg gattaaagaa gttacactgc tgggacaaaa tgttaactcc
     1861 tatggtaaaa cgctggctaa tggattgaat tttacggctt tgattaaaaa aatcggcaaa
     1921 atttccggca tcaaaaggat cagatttaca acttcgcatc cgcgtgacct ttccgaagaa
     1981 ttgattaatt gctttgccga agagggaaaa ttatgcgacc atattcacct tccggtacaa
     2041 tcaggatcaa accgggtgtt ggcattaatg aataggggtt acactgttga
//
LOCUS       DASVIM010000031         3196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1035928, whole genome shotgun sequence.
ACCESSION   DASVIM010000031 DASVIM010000000
VERSION     DASVIM010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035928"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VF305_00620"
     CDS             <1..794
                     /locus_tag="VF305_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7415667.1"
                     /translation="RHAERCVAKTTKTLRIFMSTEKIYNATINKIMNNFKEREREFQH
                     LDIGSGSGDLVKLLKKQLNTKSSCCDYTDELIQLPGQKVDIIDLNKIKELPYQDNTFD
                     IITATEVIEHLEDFRKILREMYRVLKPDGMCILSTPNILNLNSRLRNLWFGFADLMGP
                     LPINERKIESCAGHINPVSLFYIMHALYKSRFRKINFTVDKYQRSGMGKAIILWLPIK
                     LLGIKIWRREVKKNKTIDASTKYIVSKLNSLPILLGRTIIVSAIK"
     gene            1144..2118
                     /locus_tag="VF305_00625"
     CDS             1144..2118
                     /locus_tag="VF305_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX7415668.1"
                     /translation="MILIFLVIGAVSLWVGSVHIDFGHVLHYLAKALTGNFSITSQEE
                     LIIFSVRLPRIIFAGIVGASLSVGGVIFQAILRNPLADPYVLGISGGSALGAIVGIVT
                     GAGSFYLGIPLLAFLGALATVFLVFIVAGGTRGPLLDNSLLLSGVVVNAFFSAAILFF
                     LSVVNSMELHSITFWLMGDLSMASMKEIYMAAFCLIAGFVFLYAQARKLNLIVQGEET
                     ALHLGVNVERTKQWLLIVTSLITAVAVSLAGIIGFVGIMVPHLMRLVFGSDHRLLLPV
                     SALFGACFLVIADTLARVVLAPAELPVGVITALCGAPYFIFLLKRKAR"
     gene            2121..2897
                     /locus_tag="VF305_00630"
     CDS             2121..2897
                     /locus_tag="VF305_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7415669.1"
                     /translation="MSIICAENINFSYAAKQVMEDVSFAVDEAQIVAIIGPNGSGKTT
                     LLKIINGTLFPNTGQMLIDGKDTSRWSRQEIARKVAIVPQETAVIFPFYAEEIVLMGR
                     FPHLSNYRFEDKKDYRIVHEAMEKTDTLAFATRRFDELSAGERQRVLIARALAQEPKV
                     LLLDESTVFLDLKHQLQFLTLLRQLNTAQQLTVIFVTHDINLAAQNADRIILLYSRKI
                     YAIGKPAEVITAANIKEVYDVGVLIDKNPQSGLPRMTLFA"
     regulatory      2878..3055
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          883 a          661 c          720 g          932 t
ORIGIN      
        1 accgccacgc tgagcgttgt gtggcgaaaa ccactaaaac actgaggata tttatgagta
       61 ctgaaaaaat ttataacgca acaataaaca aaattatgaa taatttcaaa gagagagaga
      121 gagaatttca gcatttagat attggttcag ggtctggaga tttagtgaaa ttgttaaaaa
      181 aacaattaaa tacgaaatct tcatgttgtg actatactga tgaacttata caattacctg
      241 gtcagaaagt tgatattatt gatttaaata aaataaaaga acttccatat caagacaata
      301 cattcgatat tataacagcg acagaagtta ttgaacactt agaggatttt cgtaaaatat
      361 tacgtgaaat gtatcgtgtg cttaagccag atggaatgtg catattaagc accccgaata
      421 ttctcaattt aaattcacgg ttacgaaatt tatggtttgg ctttgccgac ctaatggggc
      481 ctctccccat caatgaaagg aaaatagaat cgtgcgctgg tcatattaat cccgtatcac
      541 ttttttatat aatgcatgca ttatataaat cacgtttccg aaaaatcaat tttacagtag
      601 ataagtacca aagatccggt atggggaaag cgataattct ttggctgcct ataaaattgc
      661 tcgggattaa aatatggagg cgagaagtaa aaaaaaataa aactattgat gcttccacca
      721 agtatatagt tagcaaattg aattcattgc ctattctttt gggtcgtaca attatagtat
      781 ctgcaataaa ataggggaaa gccacacaac aatcggctga agtcggattg caattccgct
      841 gcgctccatt gcacccgctt agccgtggcg ttgccgccgc gctgcgcacg cactcgccat
      901 tatgcagaca acttaacatt aaagggtccc aatagttatt cagcaaacaa aaactaacaa
      961 gaatatttat tcaattatct tgcgatgaaa ttaattttcc accatatttc atatctgtat
     1021 aactttttgt tgtggaaaac acatcaaaga gaatatataa ggcaagaaat ataaaaaaag
     1081 aggaggaagc cgcctattta ccgccgcgta actttatcgc gagtactgca aatcagtgca
     1141 ttgatgattt taatcttttt ggtcattggc gctgtttccc tttgggtcgg tagtgtccat
     1201 atcgattttg gtcatgtatt gcactacctg gccaaggcgc tgacagggaa tttctctata
     1261 acttctcagg aggaattaat tatcttttcc gtgcgcctgc cgcggattat ttttgccgga
     1321 attgttggcg cctctctttc cgtgggcggg gttatttttc aagcaatact gcgcaatccg
     1381 ctggccgatc cctacgtttt aggtatatcc ggtggctcgg cgctgggggc gattgtggga
     1441 atagttaccg gcgcgggctc tttttattta ggaataccgc tactggcttt tttaggggca
     1501 ctggcgacgg tttttctggt gtttattgtg gccggaggaa ccagaggccc tcttttggat
     1561 aattcgcttt tactctccgg cgttgtcgtg aacgcctttt tttccgcggc cattctattt
     1621 ttcctctctg ttgtcaatag tatggagttg cacagcatca ctttttggct gatgggcgat
     1681 ctatcaatgg cgtcaatgaa ggaaatctat atggccgctt tctgtcttat tgccggtttt
     1741 gtgtttcttt acgcgcaggc gcgcaagctg aatttaattg tgcaaggtga agaaactgct
     1801 ctgcacttag gtgttaatgt agagcggaca aaacaatggc tgctgattgt aacttctctt
     1861 attaccgccg tggccgtatc tttggccggc attatcggct ttgtaggtat tatggtgccg
     1921 cacttgatgc gccttgtttt cggttccgat caccgcttat tgttaccagt ctccgctctc
     1981 ttcggcgctt gctttttggt tattgccgat actctggccc gagtagtttt agcgccggcc
     2041 gaattgcctg tcggggttat cacggcgctc tgcggcgcgc cttacttcat ctttctttta
     2101 aaaaggaagg ctcgttaaac gtgtccataa tttgtgccga aaatataaat ttcagttacg
     2161 ccgcaaaaca ggtgatggaa gatgtttctt tcgccgttga tgaagcgcaa atcgtggcga
     2221 ttatcggccc taatggttcc ggtaaaacaa ctctcttgaa aattattaac ggcacgcttt
     2281 ttcccaacac ggggcaaatg ctcattgatg gtaaagacac gagccggtgg tcacgtcagg
     2341 aaattgcccg aaaagtagcc atcgtgccgc aggaaactgc cgtcattttt ccgttttacg
     2401 cggaagaaat tgtacttatg ggacgatttc cgcatttaag caattatcgc tttgaagata
     2461 aaaaagatta ccggatagtc catgaagcta tggaaaaaac ggataccctt gcttttgcca
     2521 cccggcgttt cgatgaactc agcgccggtg agcgccagcg cgtcctgatt gcccgcgctt
     2581 tagcacaaga gccgaaagta ctgcttctgg atgaaagcac tgtcttttta gacctgaaac
     2641 atcaacttca gtttctaact ttgctgaggc aattaaacac ggctcaacaa ctcacggtaa
     2701 tttttgttac ccacgacatc aatctggccg cgcaaaatgc cgaccggata attttgcttt
     2761 actccaggaa aatttatgct ataggaaaac ccgcggaagt tattaccgcg gcgaacataa
     2821 aagaggttta cgacgtcggt gttctgatag ataaaaatcc gcaaagcgga ttgcccagaa
     2881 tgacgttgtt tgcctagatg actgctaaat aaaggaagcc ggttgaagtc cggcgctgcc
     2941 ccgcaactgt aagcggaacg aaatccacat taaaccactg gtgcttcggt atcgggaagg
     3001 agtggagagt agggagccgc aagccaggaa actttatggg agtccaactt gctttaatcc
     3061 cgagggggaa aggagaagtc ccatgaagaa ggttcttgtt ttaaccctgt tgttttctgt
     3121 agttattttt tcaccgcttc aagctcaaga aaagaaagaa gaagaacctg ccgtaaaact
     3181 cagcgaagtt gtcgtt
//
LOCUS       DASVIM010000032         4327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_353616, whole genome shotgun sequence.
ACCESSION   DASVIM010000032 DASVIM010000000
VERSION     DASVIM010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353616"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VF305_00635"
     CDS             <1..406
                     /locus_tag="VF305_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7415670.1"
                     /translation="FMSHGISSKKFIELAGDAAEGIILPSGRVIVANQISNKDPQKKA
                     LLNYVNSYQKLYKVEGGHFGGHAWDAMMLLKGAMEKGGDNPAAIRDQLEKTTKFAGIG
                     GAFSYSPQDHAGLTKGAFVLVQIREGDWKLIK"
     gene            642..1526
                     /locus_tag="VF305_00640"
     CDS             642..1526
                     /locus_tag="VF305_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7415671.1"
                     /translation="MNSSRQLFQYILSGLSNGAIYALIGLGFAIIHNATGIINFAQGE
                     FVMLGGMLTVFFLVILNLPLVPAIVLAILISTIVGITFERLAIRPLKNAKPLRLIIIT
                     IGASIFIRGAAMLIWGKDTHALSAFSGNDPLHIAGATILPQHLWIFAVTILIIIASRI
                     FFNYSIVGKAMRACAYNPQAASLVGIDVKIMVLLSFVISSAIGSMAGIIIAPLTMTSY
                     DVGIMLGLKGFCAVIIGGMSSGLGTVLGGLLLGLLEALGAGFISASYKDAIAFIILLL
                     ILFIRPEGLFTKKETERV"
     gene            1602..2576
                     /locus_tag="VF305_00645"
     CDS             1602..2576
                     /locus_tag="VF305_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7415672.1"
                     /translation="MTKEKRQIIIFLIFVLAVLITPFFLQGSYFLNVLVFVGINTMLA
                     VALNLLLGYAGQISLGHAAFFGLGAYISGILTTRFSLDPWLVIILAAVCAAALAFVIG
                     FPILKLKGHYLAMATLGFGIIMYIVFNETVGWTGGPSGLSGIPNLHIGSWIFDNDLYN
                     YYLIWIITIIVMLLSINLSQSRIGRALRAIHDSEVAARVMGVNARLLKVQIFTVSALI
                     SAVAGSLYAHTMSFISPVSFGFNFSVELVTMVVIGGLGSIYGSFLGAAILTILPEFLR
                     VFQDLDIVIYGLMLILMTMFMPGGLISGIESMAENAYAKLKGKTRGNA"
     gene            2569..3321
                     /locus_tag="VF305_00650"
     CDS             2569..3321
                     /locus_tag="VF305_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7415673.1"
                     /translation="MLRLNSITKIFGGLIALEDVSLSVTGGSITGIIGPNGAGKTTLF
                     NIITGLYTQTSGEVYLGEKNISLFAPEVLAGLGLVRTFQNVELFGQMTVLENVMVGLH
                     TKSKSGIFSCAFKLPGQIKEERYIKKKSLQWLDFTGMADLAGTKAGNLPFGKGRLLEI
                     TRALAVEPRIILMDEPAAGLNSHETSELADLIRMIRKSGITVVLVEHDMELVMDICES
                     IIVLNLGKKLVEGTPREIQENPSVISAYLGEG"
     gene            3530..4273
                     /locus_tag="VF305_00655"
     CDS             3530..4273
                     /locus_tag="VF305_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7415674.1"
                     /translation="MLKIKNINTYYGQVQVLKNVSLHLTEGEIVTLIGANGAGKTTLL
                     NSLSGIVPPRSGQIIFNNVPINNLAAHRIVKMGISQVPEGRQVFKPLSVEDNLELGAY
                     LHHKTQAGKTATKKTIAMVYALFPVLRERRKQLAGTLSGGEQQMLSIGRALMANPKLL
                     LLDEPSMGLAPIVAQEIFQVIENLTREAKTTILLVEQNARAALKLANRGYVLETGRLI
                     LEGTAADLLQNKEVQRAYLGRDKKEIWER"
BASE COUNT         1137 a          872 c         1054 g         1264 t
ORIGIN      
        1 gtttatgagc catggtattt cctccaaaaa atttattgaa cttgccggtg atgccgctga
       61 gggaattatt cttccttccg gtcgtgtcat tgttgccaat caaatatcca ataaagaccc
      121 gcaaaaaaag gcgctgctta actatgtaaa tagttatcaa aagctctaca aagttgaagg
      181 aggtcatttt ggcgggcatg cctgggacgc gatgatgctg ctcaaaggcg ctatggaaaa
      241 aggcggcgac aatcctgcgg ctattcgtga tcaactggaa aagacaacaa aatttgccgg
      301 tatcggtgga gccttcagct attctccgca agaccatgcc ggattaacta aaggcgcttt
      361 tgttctggtg caaatcagag aaggcgactg gaaattgatt aaatgatgaa gtgatataaa
      421 ttagttaaac tttataagcg gtgcgggcga taatgatgcc tgcgccgtat taatatttat
      481 cgaaaaattg ccttaaactt aataccattt ctaaatcaaa gatgatattg aggcggctag
      541 gcgtattata tatatgttga ggaagccgac aacgacccag gcatgcgccg ggcaggcttt
      601 gccaataaaa atagcgcttt gatttagaat tggtattata aatgaacagc tcccggcaat
      661 tatttcaata tatcttatcc ggtctttcta acggcgccat ttacgcgctg attggtttgg
      721 gattcgccat catccataac gcaaccggca ttatcaattt cgctcaaggt gagtttgtaa
      781 tgctcggcgg aatgctgacg gtttttttct tggtcatttt aaacctgccg ctcgtgccgg
      841 cgatagttct ggccattctt atttcgacaa ttgtgggtat aacttttgaa cgtctggcca
      901 tcaggccact caagaacgcg aagccactca ggcttattat catcacgatc ggagccagca
      961 tttttatacg cggtgcggcg atgctgatct ggggaaaaga tactcatgct ctgtccgctt
     1021 tttcgggcaa cgatcctctt catatcgccg gcgcgactat ccttccccaa catctctgga
     1081 tttttgccgt aactatttta atcattattg ccagcaggat tttttttaat tacagcatcg
     1141 tcggcaaagc catgcgcgct tgcgcttata atcctcaggc ggcaagcctg gtcggcattg
     1201 atgttaaaat tatggtgtta ctatcctttg ttatcagctc ggctattggc tcaatggccg
     1261 ggataatcat cgctcctctg acgatgacat cctatgatgt gggcataatg ctggggctga
     1321 aaggattttg cgcggtgatc atcggcggaa tgagcagcgg tcttggtaca gtactgggag
     1381 gacttctgct gggcttattg gaagctttgg gcgccggttt tatctccgcc agttataaag
     1441 acgccatcgc ctttattatc ttgttgttga tactttttat ccggccggaa ggcttgttca
     1501 cgaaaaagga gacggaaagg gtttagatca atgaggctaa gcttttgtaa ggtaatgctg
     1561 tagaaaaaca cttttgccgg aaagattgcg gttgaaaaac aatgaccaaa gaaaaacgcc
     1621 agataattat ttttttaatc tttgtgctcg ccgtgctgat cacgccgttt tttcttcagg
     1681 gaagttattt tctaaacgtt ctggtttttg tcggtataaa taccatgctg gctgtggcgc
     1741 tcaatctttt actgggatat gccggtcaga tttcgcttgg gcatgccgcc ttctttggtt
     1801 tgggagctta tatatccggc atactcacaa ctcgattttc ccttgatcct tggctggtaa
     1861 ttattctggc ggctgtttgt gcggcggcac tggcgtttgt tatcggtttt cctattttaa
     1921 agctcaaagg tcattacctg gcgatggcca cactaggctt cggtattatc atgtatattg
     1981 tttttaatga aactgtcggc tggaccggcg gcccatcggg attatccggc atacccaatc
     2041 tccatatcgg ttcttggatt ttcgacaatg atttgtataa ttactattta atctggataa
     2101 tcactattat tgtaatgttg ttatcgataa atttgtctca atcgagaatc ggcagagctc
     2161 tgagagccat tcatgattcg gaggtggcag ccagggttat gggtgtaaat gcccggcttt
     2221 taaaagtgca aatcttcact gtctctgctc ttatttcagc tgtggccgga agcctgtatg
     2281 ctcataccat gtcttttatt tctccggtat cctttgggtt caacttttcc gtggagttgg
     2341 ttacaatggt ggttatcggt ggtctcggca gcatttacgg ctcattcctt ggtgcggcaa
     2401 ttttaacgat attaccggaa tttttgcgcg tatttcagga tctggacatt gtaatttatg
     2461 gattgatgtt gattttaatg actatgttta tgcccggagg ccttatcagc ggtattgagt
     2521 caatggcaga aaatgcctac gccaaactga aaggaaagac acgcggcaat gcttagacta
     2581 aactctataa ccaaaatatt cggcgggttg attgcgctgg aagatgtttc actttccgtg
     2641 accggcgggt cgataaccgg cattatcggt cctaacgggg cggggaagac aacgctattc
     2701 aatattatta caggccttta tacccagaca tcgggagagg tttatcttgg tgaaaaaaat
     2761 atttcgctct ttgcgccgga ggttcttgcc ggtctgggtc tggtgcgcac atttcaaaat
     2821 gtggaactgt ttgggcaaat gacagttctg gaaaatgtta tggttggact tcacaccaag
     2881 agtaagagcg gtattttttc ctgtgctttc aagctgcccg gccaaattaa agaagaaagg
     2941 tatattaaga agaaaagttt gcagtggctg gattttaccg gcatggcgga tttggccggt
     3001 accaaggccg gtaatttgcc tttcggtaag ggaaggctgt tggaaattac ccgggcgttg
     3061 gctgtggaac cccggattat tttaatggac gagccggcgg cggggttaaa tagccatgaa
     3121 acatccgaac ttgctgatct catccgcatg atcaggaaat ccggtataac agtagtgctc
     3181 gtggagcacg atatggagct ggttatggat atttgtgaat ccatcattgt tttgaatttg
     3241 gggaaaaaat tagtggaagg aacgccgcgg gaaattcagg aaaatccctc tgttatttct
     3301 gcgtatttag gcgaaggctg atagctttgt actttcgaaa atagagggat atattcattt
     3361 aaggttgccg tgttatatgg aactaaaagt tgcaaattta aagcgacttt gtaaaaagtt
     3421 ccagaggcaa ggcgtgcaaa tattgaggaa cgaggcgtac tttttagcgt acgccgcagt
     3481 gacgaaatat gcagcacaac gctgcatatg gattttttac aaggttgcta tgttgaagat
     3541 aaaaaacatc aatacatact acggccaagt gcaagtcctg aaaaacgttt ctttgcatct
     3601 gactgaagga gaaatagtca ctcttattgg cgctaatggc gccgggaaga caacgctcct
     3661 taattcttta tccggaattg tgcctccccg cagcggtcaa attattttta ataatgttcc
     3721 cataaacaat cttgccgcac atcgaattgt caagatgggt atttcccagg ttccggaggg
     3781 aagacaggtc tttaaacctc tgtctgtgga agataatctc gaattgggcg cctatttaca
     3841 tcacaaaacg caagcaggca aaactgcaac gaaaaaaacc atcgcaatgg tctacgcctt
     3901 gtttcctgtt cttagggaaa ggagaaagca gcttgccgga acattatcgg gaggtgagca
     3961 gcaaatgctc tccatcggta gagcgcttat ggccaaccca aaacttctgc ttttagatga
     4021 gccatcaatg ggactcgcgc ctatcgtggc tcaggaaatt tttcaggtta ttgaaaacct
     4081 gacccgggag gcgaaaacaa ctattcttct ggtcgaacaa aatgcgcggg cagccctgaa
     4141 gttggcaaat cgcggatatg ttctggaaac aggccggttg attctagaag gtacggccgc
     4201 cgacttatta caaaacaaag aagttcaacg ggcatatctc ggtagagata agaaagagat
     4261 atgggaacga taaaaattat gaaagcaggc gaaagtgtaa atttttatta agctgttcaa
     4321 aaatatc
//
LOCUS       DASVIM010000033         2998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1207054, whole genome shotgun sequence.
ACCESSION   DASVIM010000033 DASVIM010000000
VERSION     DASVIM010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207054"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VF305_00660"
     CDS             complement(<1..585)
                     /locus_tag="VF305_00660"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415675.1"
                     /translation="MKNKKRLGEMLVEGGLLTEDKLKQALVDQKKAGLRLGQYLTRQG
                     IVNEQQIIDLLSQQLNIRKYHPDDFPLDVGLVRYIPIETAQKNQVAPLKKKGRLLTIA
                     IVDPLDINTLDSIELLANAEVEPVICSEREVNQLINSLYGMQSGLGGIMENMEIESQP
                     DMQAEEEPDKEEVQIASLQDMAGEAPVVRLANSIF"
     gene            complement(609..2183)
                     /locus_tag="VF305_00665"
     CDS             complement(609..2183)
                     /locus_tag="VF305_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7415676.1"
                     /translation="MDYYNILNFKKEPFSNSPEPEFLFESPQHTGCLQKLELAVRLRR
                     GLNVVIGDIGTGKTTLCRKLIQNFSSTPADSTEIETHLLLDPSFDSAIEFLQIVALML
                     GIRDSNDGESEWHLKERIKDYLFSKGVNEERIVVLVVDEGQKIPENCLEILREFLNYE
                     TNNFKLLQIIIFAQKEFQTILKRRANLFDRVNLLYYLKPLNFKQMQAMVKYRISVARN
                     FEIAPSLFSFWGHAAIYLATGGYPRKIVSLCHQVILKLIIRNKKKAGWFLVRSCVSGM
                     EAPLFRRTKWALVSFLIVIALGFSIRAIILQIQNNNAYKQLSTTQIIPEVKQLTLTPI
                     APDPIMKTTSLPDSSIIQEDKNQDNKMPDYIGMLTMKKGMTLSWTLYNIYGAASSETF
                     GAVIKANPHIKNKDIIMQGDIVTLPAIPAGVQPVKNDDFIILLKQGKNIEMMYDFFRS
                     NRYRQNMPPIVFFPFWNKKEEGVTFAVVIDKCFDNDREAQKAISKLPPAIAANTKILS
                     QGDANTVFFNRRALQR"
     gene            complement(2216..>2998)
                     /locus_tag="VF305_00670"
     CDS             complement(2216..>2998)
                     /locus_tag="VF305_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HEX7415677.1"
                     /translation="SPTSGSYTPTATGPTGIAGQGFGVNFPAANLAAAASGSLGLIFG
                     TIGGNLLEIQLQALQKDSKLNILSSPSITTLDNQKAFTENGERVPYVTQQTSSGTTTF
                     TTVFVDVVMRLEITPHVIDGKNLKMTIFVQKDDVDISRKDSLGDPYIIKKQTQTVLIV
                     NDGETIVISGLTKKTRSDGDTGLPGLKDVPVLGWLFKSDSKDDKMEEVLIFITPHILQ
                     LGKIDKNAEPVESDVTQKNGTRPKQENSGTTPENKTDLDSKQ"
BASE COUNT          827 a          647 c          555 g          969 t
ORIGIN      
        1 aaaaatagaa ttagccaaac ggacaactgg cgcttctccc gccatatcct gtagtgaggc
       61 gatttggact tcttccttgt ccggctcttc ttcagcctgc atatccggtt gagattctat
      121 ttccatgttt tccataatac cgcccaaacc ggattgcatg ccatacagac tattaatgag
      181 ctgattgact tccctttccg aacaaattac cggttccact tctgcattgg ctaacaattc
      241 aatggaatcg agggtattta tatccagcgg atcaacaatg gcgatcgtca ataaacgccc
      301 cttctttttt aaaggagcta cttggttttt ttgcgcggtt tcaatcggta tataacgcac
      361 taaaccaacg tccaacggaa aatcgtccgg atgatatttc cggatattga gttgttggct
      421 taataaatct attatttgtt gttcgttgac gattccttga cgcgttaaat actggccaag
      481 ccttaaaccg gctttctttt gatcaaccag ggcttgcttg agcttatctt cggtcaaaag
      541 cccgccttct acaagcattt cacccaaacg tttcttattt ttcatttagc ctccggacga
      601 ccttctcctc aacgctgcag agctcggcgg ttgaaaaaaa cagtatttgc atccccttgg
      661 gataaaattt ttgtattagc ggcaattgcc ggtggtaatt tgctaatcgc tttctgagcc
      721 tcacgatcat tatcaaaaca tttgtcaata acaaccgcaa aggtgactcc ttcttctttt
      781 ttattccaga agggaaagaa aactatggga ggcatgtttt gccggtaacg attactccta
      841 aaaaagtcat acatcatttc tatgtttttc ccttgtttca acagtataat aaaatcatca
      901 tttttgactg gctgtacgcc agcaggaatt gccggaagag ttacaatatc tccttgcata
      961 attatatctt tatttttaat atgaggatta gctttaatca ccgctccgaa agtctctgaa
     1021 ctcgctgcgc catagatatt atacagtgtc cacgaaaggg tcatcccttt tttcattgtt
     1081 aacattccaa tgtaatcagg cattttattg tcctgatttt tatcttcctg aataattgat
     1141 gaatcaggaa gtgaagttgt tttcattatg ggatcaggag caataggagt tagcgttaat
     1201 tgtttaacct caggaataat ttgagttgtt gataattgtt tatacgcatt attattttga
     1261 atttgtaata taattgccct tatcgaaaaa cccagagcaa tgacaatcaa aaagctcact
     1321 aacgcccatt ttgttcttct aaataatggc gcctccatcc cgcttacgca acttctgacc
     1381 aagaaccatc ctgctttttt cttgttgcga ataatcagct tcaaaattac ctgatggcaa
     1441 agagaaacaa ttttacgcgg atagccgccg gtggctagat aaatcgccgc atgcccccaa
     1501 aagctaaata gagagggcgc aatttcaaag ttcctcgcta cactgatgcg atatttaacc
     1561 attgcctgca tttgtttaaa attcagcggt ttaagatagt agagcagatt aacccgatca
     1621 aaaagattgg cgcgtctttt aaggattgtt tggaattcct tttgagcaaa gataattatt
     1681 tgcagcagtt taaaattatt tgtttcataa ttaaggaatt cacgcaagat ctccaggcaa
     1741 ttctcaggta ttttttggcc ttcgtcaacg accaagacaa caattctttc ttcatttaca
     1801 cctttgctaa agagataatc tttaattctt tcttttaaat gccattcact ttcgccatca
     1861 ttggaatctc taattcccag catcaaggca actatttgca gaaactcaat agcgctgtca
     1921 aacgacggat caagcaaaag gtgggtttca atttccgttg aatctgccgg agtagaagaa
     1981 aaattctgga taagtttacg gcataaagta gttttcccgg taccgatgtc ccctatgacc
     2041 acattaagtc cgcggcgcag tcgaacagct aattccagct tttgtaaaca accggtatgc
     2101 tgtggcgatt caaaaagaaa ttccggctcc ggcgagttgg aaaagggttc ttttttaaaa
     2161 ttgagaatat tgtaataatc catttttaaa cgccatctcg ttgtttaaac ctcggctatt
     2221 gcttagaatc caaatccgtt ttgttttccg gtgttgttcc ggaattttct tgtttaggcc
     2281 tagtcccatt tttttgtgta acatctgatt caaccggctc ggcatttttg tcaattttac
     2341 ctaattgcaa tatatgcggc gttataaaaa ttagcacttc ttccatttta tcatctttac
     2401 tatccgactt aaaaagccag cccaaaacag gaacatcttt taaccctggc aatccggtat
     2461 ccccatccga tctggttttt ttggttaaac cggaaataac aattgtttcg ccatcgttaa
     2521 caatcaagac agtttgtgtt tgctttttga taatataagg atctcccaag ctgtctttcc
     2581 ttgatatatc cacgtcgtcc ttttgcacaa aaattgtcat ttttaaattt ttaccgtcaa
     2641 ttacatgcgg cgtgatttcc aaacgcataa cgacatctac aaagacggta gtgaaagtgg
     2701 tagtaccgct acttgtttgt tgtgttacat aaggaactct ttcgccattt tccgtgaacg
     2761 ctttttgatt gtctaatgtc gttatggacg gactggagag aatatttaac ttactatctt
     2821 tttgcagggc ctgcagttgg atttccagca gattcccgcc tatagtaccg aaaatcagac
     2881 ccaaagaacc ggaagcagca gctgctagat tggcagcggg gaaatttact ccaaaacctt
     2941 gaccagctat tcctgtaggc ccagtggccg ttggggtata actaccggac gttggact
//
LOCUS       DASVIM010000034         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1122831, whole genome shotgun sequence.
ACCESSION   DASVIM010000034 DASVIM010000000
VERSION     DASVIM010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122831"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VF305_00675"
     CDS             <1..211
                     /locus_tag="VF305_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HEX7415678.1"
                     /translation="EIEVGKHGLYIVRGYNSGLLLPQVAVEYKWNRESFLNETCYKAG
                     LPAQAWMDEETEIYIFSADYFDDTD"
     gene            complement(654..>2863)
                     /locus_tag="VF305_00680"
     CDS             complement(654..>2863)
                     /locus_tag="VF305_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7415679.1"
                     /translation="INNAESMKDIIIDIGEDIRNLFNIHVLTIYIFDKIKKEIYSMRV
                     ENSTIKEVRLPINNFSIAGYVANNKKMVLITDVYNERELKSINEQLKFDSSSDKKLGV
                     LSGQVLAIPILYEHNTMGVMEAITQKGGDKIDDYRQIFLDEICSVLGIAFYNQEKFSK
                     QRRHSKFDYIIRHGHITQAYIDKATEEAREQKEPIENVLINKYKVSKDNIGTSLAEYY
                     NCQFITFNDKLPIPGELLRNLTKPYLKRELWVPLERVDGRIRVLVDDPTNIIKRDTIE
                     GLLKTKSVKYDVSLSDDILKYIDRFYSSPQDDSSIDEILGKLEQDEAVSEEENGEIIT
                     ESHSVIMQLVNRIINDAYSRRASDIHIEPNIDTKNVDVRYRIDGECSLYQTLPYNYRA
                     AVVSRIKIMSNLDITVKRLPQDGKIKYKRAGDEEIELRVATIPTQGGVEDIVMRILAK
                     GKTMPLDVMALSKRNYSEMLRIMEQPYGMILCVGPTGSGKTTTLHAGLKQINTPGRKI
                     WTAEDPVEITQHGLRQVQVQPKIGFDFAAAMRSFLRADPDVIMVGEMRDFETAKIGVE
                     ASLTGHLVLSTLHTNSAPETIVRLLDMGIDPLNFADALLGILAQRLVRTLCKKCKEAY
                     HPSEEEYEEIAETYGKESFAKLNIPYTNELTLYRPKGCDACDGTSYHGRMGIHELLVA
                     SDNIKKMIQKHEPAEALRQAAITEGMTTLLQDGIIKAIQGHTDVKQVRRVCIK"
BASE COUNT          764 a          553 c          557 g          989 t
ORIGIN      
        1 tgaaattgaa gtgggaaagc acggtttata cattgtccgc ggttacaatt ccggtttatt
       61 attgccacaa gtcgcagtcg agtataaatg gaacagagaa tcttttttaa atgagacttg
      121 ttacaaggcc ggcctgccag cgcaggcttg gatggatgag gaaacggaaa tttatatttt
      181 ttcggcagat tattttgacg atacggatta gctggtcgtt aattatttta cctattccga
      241 cacaggaacg ttggttatat ttatggcaat cttgtattta ttagcagtca gatgattggt
      301 tggtatatga aagatagatt gacgtaggaa atttgttcta aaagatgctt aatcaaattt
      361 tagggtggag aaaaattaaa tccgaaattt atatatttca tttttaggtt ttcttacgac
      421 tcttgaattt gagaacaaaa tgtcggataa agttgcttaa gtctatgttt attttttctg
      481 aaaaagatca tggtgctctt ttatgtcatg atgctcttga ttacaaattt cgtcgttttt
      541 ttaatatttt catgcaccgt aagagaataa atataattct gaagcacttc cagcgttata
      601 tttattgagt caactaattt ttgcataatt ttaacattct tcaataatta atcttatttt
      661 atacacacgc gtctcacttg cttcacgtct gtatggccct gaatagcttt aataatgccg
      721 tcttgcaaca gagtggtcat tccttcagtg attgctgcct gacggagagc ttccgcgggt
      781 tcgtgttttt gaatcatttt tttaatatta tccgaggcga caagaagttc atgaatgccc
      841 attcggccat gatagcttgt tccatcgcaa gcatcgcaac ctttcggacg ataaagtgtt
      901 aactcgttag tataaggaat attcaactta gcaaaagatt cttttccata agtttcggca
      961 atttcttcat attcttcttc actggggtga taagcttcct tgcatttttt acatagtgtt
     1021 cggacaagcc gctgtgccag tatgccgagt agagcatcag cgaagtttaa tggatcgatg
     1081 cccatatcca atagccggac aatcgtttcc ggtgcgctat tagtgtgcag ggtgcttaaa
     1141 acaagatgtc cggtaagcga cgcttccacg ccaattttag ctgtttcaaa atcacgcatt
     1201 tcgccaacca tgattacatc gggatcggca cgcaagaagg aacgcatggc cgcggcaaag
     1261 tcaaatccta ttttcggttg cacttgcact tgtctaagcc cgtgctgggt gatttccaca
     1321 ggatcttcgg ccgtccatat tttcctgccc ggagtattga tctgcttgag acccgcatgc
     1381 agggttgttg ttttaccgga gcctgtggga ccaacgcata aaatcattcc gtagggctgt
     1441 tccattattc tcaacatttc cgagtaattt cgtttggata gcgccataac atccagaggc
     1501 attgtttttc ctttggccag aatacgcata actatgtctt ccaccccacc ttgagtaggt
     1561 attgtggcga cgcgtaattc gatttcttca tcacccgccc gcttatattt gatttttccg
     1621 tcctgcggta atctttttac ggtgatatcg aggttagaca ttattttaat acgggagaca
     1681 accgccgcac ggtaattata tggtaatgtc tgataaaggg aacattctcc atcaatgcgg
     1741 taacgtacgt cgacgttttt agtatcgata ttaggttcaa tatgaatgtc cgacgcacga
     1801 cggctataag cgtcatttat aattctattg actaattgca tgatgacact gtgcgattct
     1861 gtgataattt ctccattttc ttcttcactg accgcttcat cttgttccaa ttttcctagt
     1921 atttcatcta tggatgaatc atcctgcggc gatgagtaaa aacgatcaat atatttcaaa
     1981 atgtcatcag ataaagaaac atcatattta actgattttg tttttaagag gccttcaatg
     2041 gtatctcttt taataatatt cgtaggatca tcgacaagaa cacggattct gccgtcaact
     2101 ctttccaagg gaacccatag ttcgcgcttt aaatagggct ttgtaagatt tctcaataat
     2161 tcgcccggta taggcagttt atcgttgaag gtaataaatt ggcaattata atattcggcc
     2221 agcgacgtgc caatattatc tttcgaaact ttatatttat taataagaac attttcaatc
     2281 ggctcttttt gctcgcgcgc ttcttctgtg gctttatcta tgtatgcttg ggttatatgg
     2341 ccatgtctga ttatgtaatc gaattttgaa tggcgacgct gtttgctgaa cttttcctga
     2401 ttataaaaag caattcccaa cacgctgcat atttcatcta ggaagatttg acgatagtcg
     2461 tcaattttat ccccgccttt ttgagttatt gcttccatta cgcccatggt attatgttcg
     2521 tacagaattg gaattgctaa aacttgacct gataatactc ccaacttctt gtccgagcta
     2581 ctatcgaact tcaactgttc attaatgctt tttaactcac gttcattgta aacatctgta
     2641 attagaacca tttttttgtt atttgctaca taaccggcta tagagaagtt attgatcggt
     2701 aagcggactt ctttgattgt ggaattctcg acacgcatgg aataaatttc ttttttgatc
     2761 ttatcgaata tataaattgt gagtacgtgt atgttaaaaa gattgcggat atcctctcca
     2821 atatcaataa ttatatcttt catgctttcg gcgttattga tgc
//
LOCUS       DASVIM010000035         3709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1208569, whole genome shotgun sequence.
ACCESSION   DASVIM010000035 DASVIM010000000
VERSION     DASVIM010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208569"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VF305_00685"
     CDS             <1..469
                     /locus_tag="VF305_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7415680.1"
                     /translation="TIQCDFTLPERFDLSYIGADGEKHRPVMLHRVILGAVERFMGVL
                     IEHHAGAFPLWLSPTQCIILTVTDRQIPYAETVYNKLIAADIRCEKFFENEKLGYKIR
                     QAQMQKIPYMLVIGDKEVEAQSVAPRARDGKNLDSMPVDEFIAFVQEICEKKQ"
     gene            528..1022
                     /gene="infC"
                     /locus_tag="VF305_00690"
     CDS             528..1022
                     /gene="infC"
                     /locus_tag="VF305_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX7415681.1"
                     /translation="MNKEIKSATVRVISEAGKQLGVITLEEALAHAERVSLDLVEVSS
                     AAEPPVCKIMDYGKYRYKQSKKLHDAKKGQTVIHVKEIRLRPKTEEHDIQVKIKHIKK
                     FLEKHDKVKISMMFRGREIAFTEIGRRLMDEIKSELINDCVIDQEPRLEGRSMVMIVS
                     PKKQ"
     gene            1047..1244
                     /gene="rpmI"
                     /locus_tag="VF305_00695"
     CDS             1047..1244
                     /gene="rpmI"
                     /locus_tag="VF305_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX7415682.1"
                     /translation="MPKLKTHRGAAKRFRLTAKGKVKRNKAFASHILTKKTTKRKRAL
                     RKATLVDSANYKEIKRLIPYL"
     gene            1291..1650
                     /gene="rplT"
                     /locus_tag="VF305_00700"
     CDS             1291..1650
                     /gene="rplT"
                     /locus_tag="VF305_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX7415683.1"
                     /translation="MSRVKRGINAHKKRRKILKLAKGFFGARSRLIRTATEAVNKAMK
                     YAFRDRRARKREFRRLWIARINAAARLNNISYSRLVYGMKKSGIELDRKILAELAVND
                     PQGFAQVVEMAKGEQAA"
     gene            1647..2663
                     /gene="pheS"
                     /locus_tag="VF305_00705"
     CDS             1647..2663
                     /gene="pheS"
                     /locus_tag="VF305_00705"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX7415684.1"
                     /translation="MIGELQQLENNALAELKTAITEDQFLAVRTKYLGRKGILTGLLR
                     NIAQVPDAEKPLFGKRCNEVKDLLNTKIEEALQKQAIRKKEDVLLKEKIDVTLPGRAI
                     KRGRIHPVIQIRREICSIFASFGFSIVEGPEVELDYYNFEALNIPKDHPARDMQDTFY
                     IEENVVLRTHTSPVQVRIMEKMQPPVRILSPGRVYRRDSDVSHTPMFHQIEGLLIDKD
                     VSFGDLKGILTLFLKKIFGEDTKLRFRPSFFPFTEPSAEVDICCVMCHGRGCRVCGQS
                     GWLEILGSGMVDPAVFQNVGYDPEEYTGFAFGLGLERIAMLKYGISDIRLFFENDMRF
                     LKQF"
     gene            2679..>3709
                     /gene="pheT"
                     /locus_tag="VF305_00710"
     CDS             2679..>3709
                     /gene="pheT"
                     /locus_tag="VF305_00710"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7415685.1"
                     /translation="MLVSLKWLKDYVDIYLSTEELADRLTMTGLEVDEIKLIRPQFSG
                     VVVAKIISVKPHPTADRLSLCDVTDGTEKYQVVCGAKNIKAGDIVPLAKVGAMIPGGH
                     TIKSSVLRGEKSNGMLCSEAELEIGDDSSGIWQLPSNLTLGTPLENALNLGDTVLDIG
                     ITPNRADCLSMIGIAREVAAITGKKMKIPAVKIKESSENINSITSVKIMDAGLCPRYT
                     ARLIKNIKIGPSPIWMKTRLEAAGLRAINNVVDVTNFVMLEMGQPLHAFDFRFLEKGR
                     IIVRKSKEDEEFLSLDGKSRKLPEDTLLICDGVKPVAIGGIMGGLNSEVNEDTQIVLL
                     ESAYFNPTS"
BASE COUNT         1141 a          689 c          890 g          989 t
ORIGIN      
        1 gacgattcag tgtgatttca cactaccgga aagattcgat ttgagttata tcggcgccga
       61 tggggaaaaa caccggccgg taatgttgca tcgggtaatt cttggcgctg tcgaacgttt
      121 tatgggcgtg cttattgagc atcatgccgg ggcttttccc ctttggcttt cacctaccca
      181 atgcattatc ttgacagtta cagataggca gataccttat gccgaaacag tttataataa
      241 attgatcgca gctgatatac gttgtgagaa gtttttcgag aatgagaaat taggctataa
      301 gattcgtcag gcgcagatgc agaaaatccc atacatgctg gttatcggag ataaagaagt
      361 ggaagcgcag tcagttgctc ctcgcgctcg tgacgggaaa aatttggatt ccatgccggt
      421 tgacgaattt attgcttttg tgcaggaaat atgtgagaaa aagcaataga ttgaaatctt
      481 taaaacttat tggtttcagg aggtgacata tagtaaaaga tttaagaata aataaagaaa
      541 taaaatcggc caccgttcgg gtcatcagcg aagcgggaaa gcaattgggt gtaattacgt
      601 tggaagaagc tttagcccat gcggaaagag ttagtttaga tttagtagaa gtatcttctg
      661 ctgccgaacc gccagtgtgc aagataatgg attacggcaa atatcgttac aagcaaagca
      721 aaaaactgca tgatgctaaa aagggccaga cggtcattca cgttaaggaa attcgattaa
      781 ggccgaaaac ggaagaacat gatatacagg ttaaaattaa gcatattaaa aaatttttgg
      841 agaagcacga caaagtaaaa atatcaatga tgtttagagg acgggaaatt gcttttaccg
      901 agatagggcg ccggctgatg gatgaaataa aaagcgaact aataaatgat tgtgttatcg
      961 accaagagcc gcgccttgaa ggacggagca tggtgatgat tgtttcaccg aaaaagcaat
     1021 aagaatatga taataggggt gattaaatgc caaaactaaa aacacacagg ggagcggcaa
     1081 aacgatttag gcttaccgct aaaggaaagg ttaaacgcaa taaggccttt gccagccaca
     1141 tcctcacaaa aaaaacaaca aagagaaaaa gagctttaag aaaagctacg ctggttgatt
     1201 cggcaaatta caaagaaatt aagagactta ttccttattt ataggcaata gggatgctta
     1261 tattcgttat taattaggag agaagataaa atgtctaggg taaagagggg catcaatgcc
     1321 cacaagaaaa gaagaaagat tttaaaactg gccaaaggtt tctttggcgc tcgtagccgt
     1381 ctgatcagaa cagctactga agccgtaaat aaagcgatga aatacgcttt ccgtgatcgc
     1441 cgcgcaagaa aaagagaatt tcgtcgatta tggattgcgc gcattaacgc agctgcccgt
     1501 cttaacaata tatcgtacag cagattagtt tatggcatga agaaatcggg catcgagttg
     1561 gatcgcaaaa tactggcgga acttgcagtg aatgatcccc aaggttttgc ccaagttgtt
     1621 gagatggcaa aaggcgagca ggcggcatga tcggtgagct tcaacagctg gagaataacg
     1681 ctcttgccga attaaaaacg gcaataaccg aagatcaatt tttagctgtt agaacaaaat
     1741 atctcggccg caaggggatt ctaaccggtc ttttaagaaa cattgctcaa gtccccgatg
     1801 ctgaaaagcc tttattcgga aaacgctgca atgaagtgaa ggatctgctt aacaccaaga
     1861 ttgaagaagc gcttcaaaag caagccattc ggaaaaaaga agatgtcttg ctgaaagaaa
     1921 agatcgacgt cactttaccg gggagagcga ttaagcgcgg ccggatccat ccagtaattc
     1981 aaattcgtcg ggaaatatgt tctatttttg cttcttttgg tttttctatt gtcgaaggac
     2041 cggaagtaga attggattat tataactttg aagcgctaaa tattcccaag gatcacccgg
     2101 cgcgagacat gcaggacaca ttttatattg aagaaaatgt tgttttgcgc acgcatacgt
     2161 cgccggtaca agtgcggatt atggaaaaga tgcagccgcc ggtgcgaatt ttatcgcccg
     2221 gaagagttta tcggcgtgat tccgatgttt cccacactcc catgtttcat caaatcgaag
     2281 gattgcttat agataaagac gtcagttttg gtgacttaaa aggaatttta actttatttt
     2341 taaaaaagat ttttggagag gatacaaaat tgcgtttccg tcccagcttt ttccctttta
     2401 cggaaccttc ggcagaagtg gatatatgtt gtgtaatgtg ccacggacgc ggctgccgcg
     2461 tctgtgggca gagcggctgg ctggaaattt taggttcggg tatggttgat cccgccgtat
     2521 tccaaaacgt cggatatgat cctgaagaat atacgggctt tgctttcggt ctcggtctgg
     2581 aacgtatagc catgcttaag tatggtatct ctgatatccg gttatttttc gaaaacgaca
     2641 tgagattcct taaacaattt taggagagca tttctcttat gctggtcagc ctcaaatggc
     2701 ttaaagatta tgttgatatt tatttgtcaa cggaagaact tgccgatcgt ctaaccatga
     2761 ccggtttgga agtagacgag atcaaattaa ttcgtcctca attcagcggt gttgttgtcg
     2821 ctaagattat ttcggtaaaa cctcacccca ctgcagatag attatctctg tgtgacgtga
     2881 ctgatggcac tgagaaatat caagttgttt gtggcgcaaa aaatatcaag gccggcgaca
     2941 ttgttccttt agccaaagta ggggcaatga ttcctggagg acatactatc aagtcctcag
     3001 tcttgcgtgg tgagaaatca aacggcatgc tctgttctga ggcggagttg gaaatcggcg
     3061 acgattcctc cggcatctgg caactgcctt ccaatttgac tttaggtaca ccattagaga
     3121 atgctcttaa tctaggcgat actgttttag atatcggcat tactcctaat cgtgccgatt
     3181 gtttgagtat gatcggtatt gcccgagaag tcgctgcaat taccggcaaa aagatgaaga
     3241 taccggccgt taagatcaaa gaatcatccg aaaatataaa ttcaatcact tcagtcaaaa
     3301 taatggatgc aggcctttgc ccgcgttaca cagcgcggct gattaaaaat ataaaaattg
     3361 gaccttcgcc gatatggatg aaaacaagat tagaggcagc agggctgcgg gcaataaata
     3421 atgttgttga tgtaactaat ttcgttatgc tggagatggg acagcccctg cacgcttttg
     3481 actttcgttt tttggaaaaa ggccggatca ttgtccgcaa gtccaaagaa gatgaagaat
     3541 tcctttcgct tgatggcaaa agccgcaagc ttcctgagga tactttattg atttgtgacg
     3601 gtgtaaagcc cgtggctatc ggtggaatca tgggcggcct caactccgag gttaacgagg
     3661 atacacagat tgtattattg gaaagtgctt attttaatcc cacttctat
//
LOCUS       DASVIM010000036         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_441218, whole genome shotgun sequence.
ACCESSION   DASVIM010000036 DASVIM010000000
VERSION     DASVIM010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441218"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..788)
                     /locus_tag="VF305_00715"
     CDS             complement(282..788)
                     /locus_tag="VF305_00715"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7415686.1"
                     /translation="MYKCKIFDVWEEEVLLPTGKSIKQSWIDHKPTVAIVAVNGKNEI
                     LLIKQYRLAVKKHLLEIPAGSLDKLEESPIACAQRELAEETGFKAASLIKLYEGYLLP
                     GYCNEYMHFFLAQDLFYEPLSPDEDEFIEIVPTKFSHVQELLKNGEIIDAKTALGIIL
                     AGNFLQQE"
     gene            complement(830..1387)
                     /gene="trxC"
                     /locus_tag="VF305_00720"
     CDS             complement(830..1387)
                     /gene="trxC"
                     /locus_tag="VF305_00720"
                     /inference="COORDINATES: protein motif:HMM:NF008229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEX7415687.1"
                     /translation="MPENVIIRCLNCGTKNRIPKKKFKGRSACGKCAAPLDDLIIRCL
                     KCGTKNRMPEDRLNQKPLCGKCHEPLIIAQHKTKPFDVMDNTFAREVLSSDMSVLVDC
                     WAPWCGPCKKLSPIIDELASDYANGVKVVKLNVDENPLTASQYGIHSIPTMLFFKEGK
                     LVEKLTGALPKEEIEKYLLSIIKTN"
     gene            complement(1440..>1705)
                     /locus_tag="VF305_00725"
     CDS             complement(1440..>1705)
                     /locus_tag="VF305_00725"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX7415688.1"
                     /translation="VSTDALAAIIGETVSPCKPEVAQRHTGYQIGGTSPFGIRKVIPV
                     FMERTIADLPKIYINAGQRGLLAQMSSAELIDILKPFMVSVAR"
BASE COUNT          450 a          365 c          367 g          523 t
ORIGIN      
        1 ttttcagcag taattaccgg tgatgtaaaa tttatttatt atgcgctccc agtctttggc
       61 taactttgtt ggcagcgtcg tcggcttcct caatccgccg cggcggacct gcccaagcct
      121 cctccttgcc gcctcgttat cctttgaaag cgaaagggta ttacgtctat tggctcttaa
      181 gaaagtctaa caaaacttta tgcctctggc aagaagcttt ccgcggcaaa aggcaaccgt
      241 taaaaagaca ttttaaaaga ctgctttatt tttccagcaa cttattcttg ttgaagaaag
      301 ttaccggcta agataatccc taaagccgtt ttagcgtcaa ttatttcacc atttttaagc
      361 aattcttgaa catgagaaaa tttggtagga acgatttcga tgaattcgtc ttcgtcggga
      421 gacagaggtt cgtaaaaaag atcctgagca agaaaaaaat gcatatattc gttgcagtat
      481 cccggaagca agtaaccttc gtagagctta ataaggctcg cggctttaaa gcctgtttct
      541 tcggccagct ccctttgagc gcaggcgata ggcgattctt ccagcttatc gagcgaaccg
      601 gccggaattt ccaaaagatg cttttttacg gcaaggcggt attgtttgat taaaagtatt
      661 tcatttttgc cgttgacggc aacaatagca acagtaggtt tatggtcaat ccagctttgt
      721 ttgattgatt ttccagtcgg cagaaggact tcttcctccc aaacgtcgaa aatcttacac
      781 ttatacatca aattcgcttt ggattttttc atgaatgttc gacttggatt caattggtct
      841 tgattataga taacaaatat ttttcgattt cttctttggg tagcgctccc gtaagttttt
      901 ctaccagctt gccttcttta aaaaaaagca tcgtcggaat gctgtggatg ccatattgcg
      961 aagcggtgag cggattttca tcaacattaa gtttgactac ctttactcca ttggcataat
     1021 cggaggctaa ctcgtctatt attggcgata atttcttgca ggggccacac cagggcgccc
     1081 agcaatcgac tagaaccgac atatcggagg acagtacttc acgggcaaat gtattatcca
     1141 ttacatcgaa aggtttggtt ttatgttgag caattataag cggttcgtgg catttgccgc
     1201 atagcggttt ttgatttagg cgatcttcgg gcattctatt ttttgtgccg catttaaggc
     1261 accgaataat caagtcatcc aatggtgcag cgcatttacc gcaagccgac cggcccttga
     1321 attttttttt gggtatgcga ttttttgtgc cgcaattaag gcagcggata ataacatttt
     1381 caggcatatt gatgtacctt taaattacca tcatttcttt ttagacagca agcaaagaat
     1441 catctggcta cgctgaccat aaacggctta agtatgtcga taagttcagc agaagacatc
     1501 tgtgccaaaa ggccgcgctg tccggcatta atataaattt tgggtaaatc ggctatggtt
     1561 ctttccatga aaacaggaat cactttccgt atgccgaaag gcgaagtacc gccgatttga
     1621 tagccggtat gccgctgtgc gacttctggc ttacaaggag aaaccgtttc gccgataatc
     1681 gctgccagtg catctgtaga aactt
//
LOCUS       DASVIM010000037         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_101891, whole genome shotgun sequence.
ACCESSION   DASVIM010000037 DASVIM010000000
VERSION     DASVIM010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101891"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VF305_00730"
     CDS             <1..346
                     /locus_tag="VF305_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415689.1"
                     /translation="LSIISYQKGKPIKSFIVRKDVKDHGTKSAIEGDVRAGEKVAIID
                     DVITTGISTITAIEQARKEGLSVQRVITLIDREEGGRENILQHIDNIESVLTRTEIMN
                     LRAKKMSGEDIK"
     gene            343..1497
                     /locus_tag="VF305_00735"
     CDS             343..1497
                     /locus_tag="VF305_00735"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEX7415690.1"
                     /translation="MKKSSAVFILHFIVLSFIGASNSGANPGIIPVTKFTSPALPGGV
                     PEGWSLEKKAGIPSLKIEKEDNIFYLHLISLGDSSFGLRKEASVDVKKFPILRWRWKV
                     KKLPMGGDVRKSSTDDQALQVYVAFKETGFPSILNTPIIGYIWDNEAPKGWTGRSRQI
                     GGNKLRYIVLRNKTDKIGQWYTEQRNIYQDYKRLFRDIKGGEPQGLTTGLQIYINSQR
                     TKSPAEGLVGEIYFSDEPSDIALAETEKAVGISAIKQPVTAKFSTERKEKPRLDCINI
                     SIDFKTDSTTVEENYADEMQTVASYLIRNPEIKLTIIGHTDNIGTEEHNLALSNRRAE
                     SAKHYLVNKFNFDSQRLLVQGAGSSQPITDNDTLEGRQQNRRVSIKDCPE"
     gene            1605..>2620
                     /locus_tag="VF305_00740"
     CDS             1605..>2620
                     /locus_tag="VF305_00740"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX7415691.1"
                     /translation="MADQLKFPQPTLQAGNELHGFKVLRVETIPAMRLTAYEIEHEKT
                     GAKVLHLHSLDRENLYAIGFRTPPTDSTGLPHILEHSVLAGSKKYPLKDVFKELMRST
                     LQTFINAFTYPDKTIYPVASQIKADFYNLARVYTDLVLHPRLLKETFFQEGHHLEFVV
                     PGDLKSDLTISGIVYNEMKGAYSSPDSLMYKAIQENLYPDSIYAYDSGGDPDVIPKLT
                     YEQFKNFHRTYYSPSNARFFIYGDIETAEHLIFLSEMLSGFEKVEVDSFIKSQPRLTE
                     PRQVQSSYPVGKKESLEHKTMVNVAWMLAENKDYETALFLEIISGLLVGSAASPLRKA
                     LIDSV"
BASE COUNT          813 a          587 c          556 g          664 t
ORIGIN      
        1 cctttccatt atctcgtatc aaaagggcaa accaataaaa tcttttattg ttcgaaaaga
       61 cgttaaagat cacggcacta aaagcgccat tgaaggcgat gttcgtgccg gcgaaaaggt
      121 ggctattatc gacgatgtta ttacaacagg catctctaca attaccgcta tcgaacaggc
      181 acgcaaagaa gggcttagtg tccaacgggt tatcacgtta attgaccgcg aggaaggcgg
      241 cagggaaaac atccttcagc atattgataa catcgaatcc gttcttacca gaaccgaaat
      301 aatgaatctg cgcgcaaaaa aaatgagcgg cgaggatatt aaatgaaaaa aagctccgcc
      361 gttttcattt tacactttat agtcttgtct tttattgggg cctcaaattc cggagcgaat
      421 cccggaatta ttccagtaac taaatttact tcaccggctc tgccgggtgg agttcccgaa
      481 ggttggagct tggagaaaaa agccggtatt ccttccctga aaatagaaaa agaagacaat
      541 atcttttacc ttcatttgat ttccctaggt gattcctctt ttggtttacg caaagaagca
      601 agcgtagacg ttaaaaaatt tcccatatta cgctggcgct ggaaagtaaa aaaactgcct
      661 atgggtggag acgtccgcaa atcatctact gatgatcaag ctttgcaagt ttatgtagca
      721 tttaaagaaa ctggtttccc ttcgatactc aatacaccta taattggcta tatctgggac
      781 aacgaggctc caaaaggctg gactggcaga agccggcaga ttggtggcaa caaattaaga
      841 tacattgtct taagaaacaa aactgataaa ataggccagt ggtacacaga gcagcgcaat
      901 atatatcaag actacaagag acttttcaga gatatcaaag gtggagagcc acagggatta
      961 accaccggct tacaaattta cattaactcc caaaggacaa aaagtcccgc cgaaggttta
     1021 gtaggcgaaa tatattttag cgatgaacct tccgatatcg ctttagctga aacagaaaaa
     1081 gccgtcggaa tatcggctat aaaacagcct gttactgcga aattttccac ggaacgaaaa
     1141 gaaaaaccaa gactagattg cattaacata agtattgatt ttaaaaccga ctcgacaaca
     1201 gtcgaagaaa attacgctga tgaaatgcaa acagttgcca gctacttgat tagaaatccc
     1261 gagattaagc tcacgataat cgggcacacg gataatattg gtaccgaaga acataatctc
     1321 gccctatcta accggcgcgc cgaaagcgca aagcattatc ttgtaaataa attcaacttc
     1381 gattcgcagc gcctgcttgt gcaaggagcc ggctcctcgc aacctatcac cgataatgat
     1441 acactcgaag gccgacagca gaatcgtcgt gtttcaatta aagattgtcc cgagtgaaaa
     1501 ttattgtaac tggcaagacg cgcattgtct gaataaatcg ccaaacgttt ttctattgca
     1561 aaagtaatcc gatgaaggca ctataataat aaaggatttc ttgtatggcc gatcaattaa
     1621 aattccccca gccgacactg caagccggaa atgaattaca cggatttaaa gtactgcgcg
     1681 tggaaacaat ccccgctatg cggctcaccg catacgagat cgagcatgaa aaaaccggcg
     1741 ctaaagttct gcatctgcat tcactggacc gggaaaattt atatgccatc ggctttcgca
     1801 cgccaccaac agactcaacg ggactgccgc atattctgga acattccgtg ttggccggtt
     1861 cgaaaaagta tcccctcaaa gacgtcttta aagaattgat gcgctccaca ttgcaaacat
     1921 ttattaacgc ttttacttac cccgacaaaa caatttatcc ggtcgccagc cagatcaaag
     1981 cggattttta caatctggcg cgtgtctata ctgatctggt tttgcatcca cgtctattga
     2041 aagaaacttt ttttcaggaa gggcatcacc tggaatttgt tgtgccgggc gatttgaaaa
     2101 gcgatctgac tatttccggt atcgtctata atgaaatgaa aggggcatac tcctcccctg
     2161 attcgctgat gtacaaagcc atccaggaaa acctttatcc ggatagtatt tacgcttacg
     2221 attccggcgg tgaccctgat gttattccca aacttaccta cgagcaattt aagaattttc
     2281 accggaccta ttattcgcca tccaatgcgc ggttttttat ctatggagat atagaaactg
     2341 ccgagcatct gatttttctt tcggagatgc tctccggatt cgagaaagtg gaagttgatt
     2401 ccttcattaa aagtcagccg cgcctgactg agcctcgcca ggtgcagagc agctatcccg
     2461 tggggaaaaa agaatcccta gagcataaaa caatggttaa tgtcgcctgg atgctggcgg
     2521 aaaataagga ttatgaaaca gcgctttttt tagagatcat ttcgggacta ctcgtcggaa
     2581 gcgccgccag ccctctgcgc aaagcgctga ttgattccgt
//
LOCUS       DASVIM010000038         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_272183, whole genome shotgun sequence.
ACCESSION   DASVIM010000038 DASVIM010000000
VERSION     DASVIM010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272183"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VF305_00745"
     CDS             complement(<1..587)
                     /locus_tag="VF305_00745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX7415692.1"
                     /translation="MASLKDIKRKVGAVQKTKQITRAMNMVAASKFKTSQLRMENFRP
                     YASKFMDVLNSLALRVESMSHPLLAVRDPKRIRVICMTSDRGLCGGFNTNLIKATERF
                     LKDKTKEDKEISLINVGRKGRDFFRKKANIIGQWVDVVSKFDMTLAVSISNDVIVPFI
                     KEEYDELYLIYNQFVNVSVQRPTVVRLFPLPSIGQ"
     gene            complement(687..2201)
                     /gene="atpA"
                     /locus_tag="VF305_00750"
     CDS             complement(687..2201)
                     /gene="atpA"
                     /locus_tag="VF305_00750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX7415693.1"
                     /translation="MEPIKAEEISQIISKQIKSYEKKLDISETGTVLSVGDGIARVYG
                     VQNAMAMELLEFPGGILGMVLNLEADNVGVAVLGEVTHIKEGDIVKRTGKIAQVPVGE
                     GLLGRIIDATGKPLDGKGPIETNEYRRIEMIAPGVIQRQPVNEPMYTGLKAIDAMTPI
                     GRGQRELIIGDRQIGKTAICVDTIIRQKDTGVKCIYVAIGQKKSTVAQVVEKLKQQDA
                     MSYTCVVAGCASDPATLQYIAAYAGCSIGEYFRDRGQDALIIYDDLSKQAVAYRQISL
                     LLRRPPGREAFPGDIFYNHSRLLERAARVSKELGSGSLTALPVIETQAGDVSAYIPTN
                     VISITDGQVYLEPSLFFSGIRPAINVGLSVSRVGGAAQVKAMKQVAGTLKLDLAQYRE
                     LAAFAQFGSDLDKATQAQLNRGVRLVELLKQPQFKPMSLSQEVVILFAGTRGFIDKYE
                     VEKVRVYEQQLLGFVESKYSEIMKEIEDKKVISPELEKKMKDVMTEFDSVFVSE"
     gene            complement(2205..2747)
                     /gene="atpH"
                     /locus_tag="VF305_00755"
     CDS             complement(2205..2747)
                     /gene="atpH"
                     /locus_tag="VF305_00755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX7415694.1"
                     /translation="MISNISKRYAHAFFEIAAEEKQLERYYNELRQCSSIIAQNKVLG
                     EFLANPIFEQESKKKVLEKIISKLQLSGITVNFLKLLVDKKRVEALPDIEAFYRQLMD
                     EALKKVRVTLKTAFPLSRKMQDYINSNMEKITGRKVEITVETDTSLLGGIVIGVGDTL
                     YDGSIKNQLNNMRNLLGEAR"
     gene            complement(2744..3340)
                     /locus_tag="VF305_00760"
     CDS             complement(2744..3340)
                     /locus_tag="VF305_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEX7415695.1"
                     /translation="MKKMFKSARLSLFFSLFLILSILVNTLYASSGEGGSNEGNKWVE
                     FAWKTFDFAVLAGFLYWLLAAKVKEFFVGRRKNIKESLENTKQQKAEAEKKYREYSEK
                     IDKASLEIDGIFEMIKTQGVAEKQKIIEDAGKVARKLKEDAQTRIEQELQKASYQLRN
                     EAVQLSVQMAEDILKRNITAQDNETMIREYMDKVVSKN"
BASE COUNT          909 a          850 c          656 g         1053 t
ORIGIN      
        1 tcttgcccaa tggacggcaa gggaaataat cgaactaccg tcggccgctg tacggaaaca
       61 ttgacaaatt gattataaat caaataaagc tcatcgtact cttctttgat aaaaggtact
      121 attacatcat tggaaatgga aactgccaag gtcatatcga acttgcttac aacatcaacc
      181 cactgtccga taatgttcgc tttttttcgg aaaaaatctc gtcctttgcg cccaacattg
      241 attaaggaaa tctccttatc ttctttggtt ttatctttta aaaatctttc cgtcgctttg
      301 attaagtttg tattaaaccc gccgcaaagg ccgcgatcgg aagtcatgca aattactctg
      361 atccttttgg gatcccgcac agccaaaagc ggatgggaca tgctttcgac acgcagggcc
      421 agagaattta gcacatccat gaatttgctg gcgtaaggac ggaaattttc catccgtaat
      481 tgtgacgtct taaacttcga tgcggcgacc atgttcattg cccgcgtaat ttgcttcgtt
      541 ttctgaacgg cgcctacttt tcttttaata tctttaagcg aggccactaa aaaattctcc
      601 tcaaataatt atttgacggc gttgtaaaaa agccaagttc gtgcacattt agaacttata
      661 aatagtccat tcgttattta acccatttat tcggaaacga agacagaatc aaactctgtc
      721 ataacgtcct tcattttctt ttccaactcg ggagaaataa ctttcttgtc ttctatttct
      781 ttcattattt ccgagtattt gctttccaca aaacccagca gctgttgctc gtagacgcgt
      841 actttttcga cttcgtattt atcgataaat cctctggttc cggcaaagag gataaccact
      901 tcttgggaaa gagacatagg cttaaattga ggttgtttca gtaattccac caaacgcaca
      961 ccacgattca attgcgcttg cgttgcttta tcgagatcgc tgccaaactg ggcaaatgcc
     1021 gccaattcac ggtattgagc aagatcaagt ttcaaagttc cagcaacctg cttcatcgcc
     1081 ttgacttgag ctgcgccacc gacgcgggaa accgacaaac caacattgat tgccggacga
     1141 atgccggaga aaaacaagct cggttccaga taaacctggc catcggtgat cgaaataacg
     1201 tttgttggaa tgtatgcgga aacgtcaccg gcttgagttt caataacagg cagggcagtc
     1261 aaagagccgc tacccagttc tttgctcacg cgcgccgcgc gttccagcag tcgggaatgg
     1321 ttataaaaaa tatcccccgg aaaagcttca cgcccgggag gacggcgcaa aagcagtgaa
     1381 atctgccggt aagccacagc ttgcttggac aaatcatcgt aaataatcaa agcatcctga
     1441 ccgcgatcac gaaaatactc gccaatactg caaccggcat aagcggcgat gtattgcagg
     1501 gttgccgggt cgctcgcaca accggcaaca acgcacgtgt aagacatggc atcttgctgt
     1561 ttcaatttct ccacaacttg cgccacggta gatttctttt ggccgatagc gacatatata
     1621 cacttaacac ctgtatcctt ctgcctaata attgtatcga cgcaaattgc cgttttgccg
     1681 atttggcggt caccgataat taattcgcgc tgcccgcggc caatcggtgt catagcatcg
     1741 atagccttca gacctgtgta cataggttca ttaacgggtt ggcgttgaat aacgcccggt
     1801 gcgatcattt caatacggcg gtactcattc gtttcaatcg ggcctttgcc gtcgaggggt
     1861 ttaccggtcg cgtcaattat tcgtcccagc aatccttcgc ccaccggaac ttgcgcaatt
     1921 ttacctgtcc ttttgacgat atcaccttcc ttaatatgtg taacttcacc taaaacagcg
     1981 actccgacat tgtccgcttc caaattgaga accatgccca aaatgccgcc cgggaattcc
     2041 aaaagctcca tcgccattgc attttgcaca ccgtaaactc tggcaatacc atcgcccacg
     2101 gataagacag ttccggtctc gcttatatcc agcttttttt catagctttt aatttgctta
     2161 gaaataattt gactaatttc ttccgcttta attggctcca tgccttatct tgcctcccct
     2221 aaaagatttc tcatattgtt cagctggttt ttgatgctgc catcgtaaag tgtatcgcca
     2281 acaccaataa ctatgcctcc taaaaggcta gtgtctgttt ccactgttat ttccactttc
     2341 cggccggtta ttttttccat attcgagttg atataatctt gcattttgcg ggaaagggga
     2401 aatgcagttt tcaaagtgac cctaactttc tttaatgctt catccattaa ctgccggtaa
     2461 aaagcttcga tatcaggtaa tgcctcgacc ctttttttat ccacaagtaa ttttagaaaa
     2521 ttgaccgtta tgcccgaaag ctgcagcttg ctgattatct tttccagaac cttttttttg
     2581 ctttcctgtt caaaaattgg attagctaaa aattcaccca aaactttatt ttgggcaata
     2641 atcgaagaac attggcgaag ttcgttataa taacgttcca attgtttttc ttctgctgca
     2701 atttcaaaaa aagcgtgagc gtagcgcttg gaaatattgc taatcaattt ttactcacca
     2761 ccttatccat gtattccctg atcatagttt cattatcttg cgcggtaata ttccttttta
     2821 agatatcttc cgccatctgt acggaaagct gcacagcctc gttgcgcaat tggtaagatg
     2881 ctttctgcaa ctcctgctca atgcgcgttt gagcatcttc tttcaatttc cgggcaactt
     2941 tccccgcatc ttcaataatc ttttgttttt cggcaactcc ttgcgtcttg atcatttcaa
     3001 agatgccgtc aatttcgagg gaagctttgt cgattttttc cgaatactcc cggtattttt
     3061 tctcagcttc ggctttttgc tgttttgtat tttccagcga ttctttaatg ttttttcgcc
     3121 gcccgacaaa aaactctttt acttttgccg ccaagagcca ataaaggaaa ccagccagca
     3181 cggcaaaatc aaatgtcttc caggcgaatt cgacccattt atttccctca ttgctgccac
     3241 cctcgccgct agaagcataa agagtgttta ctaaaattga aagaattaag aacaaagaaa
     3301 aaaataaaga cagccgtgct gatttgaaca tttttttcat tgaacgcttc tccccaacac
     3361 cttttcggca atctctaaag acagcttccg ggattggctc tctaaaattt gccgtgccgc
     3421 ttggatttct ttatccatct tttgctgaaa ttcctgcgtc atcagagg
//
LOCUS       DASVIM010000039         3868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_358241, whole genome shotgun sequence.
ACCESSION   DASVIM010000039 DASVIM010000000
VERSION     DASVIM010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358241"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2765
                     /locus_tag="VF305_00765"
     CDS             <1..2765
                     /locus_tag="VF305_00765"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7415696.1"
                     /translation="ARNFPELMAHVTDSLPLLFSAEQLEKRIKPLLEPAKIKEALAQN
                     RKSLEQLEGIGRSEMMAKDPMGFSGIILRQLSTLLPSHKAQFYQGQLISEDGKHALII
                     AKIAGSGTDTSISAKIDKLLKDTEQDLKSNQALAKDKYMLTSVGAYRAALDNEQTAKH
                     DTRIAIILTTLGIALLLLFAFPRPLIGLLALLPSVVGSITALFVCSFFFKSMSMLAIG
                     FGGAIMSFTVDLGITYLLFLDQTKETYGKQVVREVWLAELLAVLTTVGSFLLLLISDF
                     KILAEIGVFSALGVTFTFLFVLIIFPKIFPSMPPANRQSNPLLIHALQKIAAPAKWKL
                     LVMVVFGVVMLFFAHPEFDANINAMNSVSKETIDAEKTLQNTWGDFSGKCYIFLQAPS
                     MAQLQQKNDQLMPLLAADIQKEKLSPVFLPSLLFPSPAMSQKNMEAWQGFWSKERLAK
                     LKRDVASAARENGFAPSAFEPFWKIINNKNVHSGEIPEKYFEFFGITRGPVEYTQLSL
                     IATGKNYDAEDLASRLTNQGLAKIFDVGLFNNRLGNFLKSIFIQIALIIGIGTVIIIF
                     VFYLDWLLSLAVLAPIAFALVSTLGTLKIIGHPLDIPSIMLWIVIMGMGINYSIYYAC
                     FYQRYRDENHPAMDIVRLAMFLASFTTLIGFGVLAIAKHNLLRSIGLTSLLGIGYSII
                     GAYLILPTLVKKIYAPFNFPSGPLEIGSKEHWKRALLRYRHLEAYPRLFARFKIMIDP
                     MFKELDKYVQNPRRIIDIGCGYGIPAVWLLEINPSAQVYGLEPDEIRVLIASHALGTR
                     GSVEIGRAPALPAVEGFVDYVLMLDMLHLISDEELQLVLQRIYGKLEAGGTLLIRATV
                     PSDKKIPWKRWIENARLKLTGMRQRFRSEKELVAFMEKTGFVVSVHVSDTPEIEEEWF
                     VGNKTS"
     gene            2789..3667
                     /locus_tag="VF305_00770"
     CDS             2789..3667
                     /locus_tag="VF305_00770"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7415697.1"
                     /translation="MKILSYRIAIYFSRLWGAWFFHTFSWFIATGYFFLFPVRVAHSL
                     KFYRALFPGRNFSYHLYCAWKQYHNFTTVYLHRFVALNEDEIEFTKQGWEYLDEAVEK
                     KTGAIVVMSHIGNWELAAQMLSRKGLPIMLYLGAKHKEQIERMQKETLAKSGIKIVAT
                     SDKESSPFALLEGISFLREGGIVFITGDRLWGNQRAIMVNFLGHQTQLPDTPHLFALM
                     SGAPLMTFFVYQEAVGKYQIKVSRGRKVFAATRVDRKKAVLESAQAYADEVMQMAREH
                     PGQWYHFEPFLGEKIK"
BASE COUNT         1000 a          799 c          925 g         1144 t
ORIGIN      
        1 aggcgagaaa ttttcccgag cttatggcgc atgtaacaga tagtttgccc cttcttttta
       61 gcgcggaaca attagaaaaa agaataaaac cgctcttgga acctgcgaaa attaaagagg
      121 ctcttgccca aaaccggaaa agtttggagc aactggaggg aatcggccgc agtgaaatga
      181 tggccaaaga tccaatggga ttttccggca taatcctacg gcagttatcc actttgcttc
      241 cttcccataa ggctcaattt tatcagggtc aactgatttc tgaagatggt aagcatgcgc
      301 tcattatagc taaaattgcc ggatcaggga cggatacatc gatctccgct aagattgata
      361 agctattgaa agataccgag caagatttga agtccaatca agctttggcc aaagataaat
      421 acatgctgac ttcggtcggg gcatatcgag cggctttgga taatgaacaa accgccaaac
      481 atgatacgcg aattgctatt atcttaacaa cattgggcat agccctgttg ctgctttttg
      541 cttttcctcg tccgctcatt ggtcttctag cgttgttgcc ttcagtggtg ggttcaatca
      601 ctgccttgtt cgtatgttct tttttcttta aatccatgtc catgctggcc atcggctttg
      661 gtggggcgat aatgagtttt acggttgatc tgggcatcac ctatttgctt tttttggatc
      721 agacgaaaga aacatacggt aagcaggtgg tacgggaggt ctggttagcc gagcttttgg
      781 cggtgctcac gactgttggg tcttttcttc tgctgcttat cagtgatttt aaaattctcg
      841 ctgaaattgg agtcttctcc gcactgggag tcacttttac gtttcttttt gtccttatta
      901 ttttcccgaa aatatttcca tctatgccgc ctgcaaaccg tcaaagtaac cccttgctta
      961 tccatgccct tcaaaagatt gcagctccgg ctaaatggaa attgctggtg atggttgttt
     1021 tcggggtggt gatgctgttt tttgcacatc cggaatttga tgcaaatatt aatgccatga
     1081 attccgtgag taaagaaaca attgatgccg aaaagacatt acagaatact tggggtgatt
     1141 tttccggtaa atgttatata tttctgcaag cgccaagcat ggcgcagttg caacaaaaga
     1201 acgatcagtt gatgccgcta ttggcagccg atattcagaa ggaaaaactc tcccccgttt
     1261 ttcttccatc tttgttgttt ccatcaccgg caatgagcca aaaaaatatg gaagcctggc
     1321 agggcttttg gagcaaggaa cgtctggcca aactaaagcg ggatgtggct tcggctgcca
     1381 gggaaaatgg ttttgcgcct tctgccttcg agcccttttg gaaaataatc aataataaaa
     1441 atgtacactc cggagaaata ccggaaaaat attttgaatt ttttggaatt accaggggcc
     1501 cggtggaata tacgcagttg agcttaattg cgaccggtaa aaattacgat gccgaagatt
     1561 tagcttcccg tctcaccaat caggggctcg ctaaaatatt tgatgttggt ttatttaaca
     1621 atagactggg gaactttttg aaaagcattt tcatccagat tgcattgatt atagggatag
     1681 gcactgtgat cattatcttt gtgttttatc tcgactggct cctatcactg gcggtgcttg
     1741 ctcccattgc ttttgcgctt gtctctacgc tgggaacact caagatcatc ggacatccgc
     1801 tggacattcc cagcatcatg ctttggattg tcattatggg catggggatt aattattcta
     1861 tttattatgc ctgtttctac cagcgctacc gtgatgaaaa ccatcccgct atggacattg
     1921 tcaggctggc aatgtttctg gcatctttta ccacgcttat tggtttcggc gtattagcga
     1981 tcgccaagca caatcttttg cgcagtatcg gcctcacctc tcttttaggc attggctact
     2041 ccatcattgg agcctatctt attttgccga cattagtgaa gaaaatatat gcgccgttta
     2101 attttccttc ggggccattg gagattggtt caaaagagca ttggaaacgt gctttgttgc
     2161 gttaccgcca tctggaagca tatccgcggc tttttgcccg ttttaaaatc atgattgatc
     2221 cgatgtttaa agagttggat aagtatgtgc aaaacccccg ccggattatt gatatcggtt
     2281 gcgggtatgg aattccggcc gtgtggcttc tcgaaataaa tccttccgct caagtatatg
     2341 gtttggaacc ggacgaaata cgcgtgctca ttgcttctca tgccctagga actcgcggta
     2401 gtgtggaaat aggacgggct cccgctttgc ctgccgtcga aggttttgtt gactatgtgc
     2461 tgatgctcga catgctgcat ttaatatccg acgaagaact gcagcttgtt ttgcagcgta
     2521 tttacggtaa actggaggcg ggtggaactt tactgatcag ggcgacagta ccatcggata
     2581 aaaaaattcc ctggaagcgc tggatagaaa atgctcgctt aaagctgaca ggtatgcgcc
     2641 agcgtttccg tagtgaaaaa gaattagtag cttttatgga aaaaaccggt tttgttgtca
     2701 gtgttcatgt ttcggacacg ccagaaatcg aagaggaatg gtttgtcggc aataagacct
     2761 cttgaataat gactttagat tgcattttat gaaaatatta tcttaccgca tagcaattta
     2821 tttttcccgg ctttgggggg cgtggttttt ccacactttt tcctggttta ttgccaccgg
     2881 ttattttttc ctctttccgg tacgcgttgc gcacagcctg aaattttacc gggcgctttt
     2941 ccccggccgt aatttttcct atcatttata ttgtgcgtgg aagcaatatc ataatttcac
     3001 cactgtatat ctccaccggt ttgtcgcgct caatgaagac gagattgaat ttactaaaca
     3061 aggctgggag tatttggatg aggcagtgga aaaaaagacc ggcgcgattg tggtgatgtc
     3121 gcatatcggc aactgggaac tggcggcgca aatgttaagc cgcaagggac tgcctattat
     3181 gttgtatctt ggtgcaaagc ataaagagca gattgagcgc atgcaaaagg aaactctggc
     3241 gaaaagcggg attaaaatag tggcgacgtc ggataaagaa agttcgcctt ttgctcttct
     3301 ggaaggaata agttttctgc gggagggcgg gattgttttt atcaccggcg accgtctatg
     3361 gggtaatcag cgggcgataa tggtcaattt tctggggcat cagacgcaac tgcccgacac
     3421 acctcatttg tttgctttaa tgtcgggcgc accgttgatg acttttttcg tgtatcagga
     3481 ggctgtcgga aaataccaga tcaaagtttc cagggggcgg aaggttttcg ccgccacgcg
     3541 cgtggatcga aaaaaggcgg tgctggaatc ggctcaagct tatgccgatg aagttatgca
     3601 aatggcccgt gaacaccccg gccaatggta tcattttgaa ccctttctag gcgaaaaaat
     3661 taaataaaaa agttttgatc gaacagcata aaatttgctt tgcgcccatt taaaattagt
     3721 ataagaagat atttaataca ttgaaatatg ctctaggaaa cccattaaca ggagcgaaca
     3781 atgaaagcta atgaagttcc gcaggacatt acgtattatg aaggtgagaa acgcgcctgc
     3841 tatgctctca atgatgaagg gaaatatg
//
LOCUS       DASVIM010000040         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_358539, whole genome shotgun sequence.
ACCESSION   DASVIM010000040 DASVIM010000000
VERSION     DASVIM010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358539"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VF305_00775"
     CDS             complement(<1..365)
                     /locus_tag="VF305_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family N-terminal domain-containing
                     protein"
                     /protein_id="HEX7415698.1"
                     /translation="MKIRIPFAVLPIFFLLAGCLKQADTVKDVIDLRQDHASYLSDTD
                     KTKDILAAETQSMMDEDYNIIYFSVWHQRQPFHALPERVSLDFKKFSLKLGYGENKIK
                     HSKAWIKKLQQNAFLENYP"
     gene            543..1028
                     /locus_tag="VF305_00780"
     CDS             543..1028
                     /locus_tag="VF305_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7415699.1"
                     /translation="MAVKILLKDFAFFKDFSDDQLKKLASLAKEENHLAGTHMYQKGD
                     VARSLYLIKEGKVALFMDTDMGHNKPPLQVMVDIITTGESLGWSAVVEPYSYTLAACC
                     LDNTKFIAFDAFQLRKLMEEDCALGFMIMQAVAKVISSRLNHTRIILVGERGLSVLTE
                     Y"
     gene            1058..1264
                     /locus_tag="VF305_00785"
     CDS             1058..1264
                     /locus_tag="VF305_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1094..1096,aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HEX7415700.1"
                     /translation="MADQIIIYGKVGUPYTDKARSAFGDKAIYLDVTANTDQLAEMLK
                     ISGGTRQVPVIVENGKVTIGYGGT"
     gene            1322..1789
                     /locus_tag="VF305_00790"
     CDS             1322..1789
                     /locus_tag="VF305_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX7415701.1"
                     /translation="MKHPNLDAIIEKAIANEEEANQFYLKLSEIVTDKSAKDTLLYLA
                     KEEKKHKEYLLHYRQESFIGNATKSSEMTNYKIAEFLEPPAVQFNMDSKDVYLVAAER
                     ELNSYNFYMGLAAIHPDGEIKDMLMKMAAQELKHKEKVEYLYSNTAFVQTAGG"
BASE COUNT          608 a          326 c          413 g          537 t
ORIGIN      
        1 tggggatagt tttctaaaaa agcattttgc tggagttttt ttatccaggc tttagaatgt
       61 tttattttgt tttcgccata accgagtttc aggctgaatt ttttaaaatc aagagatacc
      121 ctttcgggca aagcatgaaa aggctgacgc tggtgccaaa cagaaaagta aatgatgtta
      181 tagtcttcat ccatcattga ttgagtttct gctgcaagaa tatcttttgt cttgtccgta
      241 tcactcagat aagaagcatg atcttgacgc aagtcaatta cgtctttgac agtatctgcc
      301 tgcttcaaac aaccggccag caaaaagaaa atcggcagaa cagcgaaagg aattcgtatt
      361 ttcataaaaa aagaccttga tttaatttta tagtttttta tagtcttttt ttaagttttg
      421 agcaagatta aagatcgatg aacgattttt ttcaacagtt gataaataat tttgtggcat
      481 aagatatcga acgctgatat aaatgaaacg aatatttcag acgagaagaa aagagttagt
      541 ttatggccgt aaaaatcctc ttaaaggact ttgccttttt taaagatttt agtgatgacc
      601 agttaaaaaa attggcatct ttggctaaag aagaaaatca tttagccggt acgcatatgt
      661 accaaaaagg agatgtggcc agaagtcttt acctgattaa agagggtaag gttgccctgt
      721 ttatggatac tgacatgggg cacaacaagc ctcccctgca ggttatggtg gatataatca
      781 ccacaggaga atcgctgggc tggtctgctg ttgtagagcc atattcatac acgcttgcgg
      841 cttgctgcct tgataatacg aagtttattg cttttgacgc atttcagttg cgtaaactaa
      901 tggaagaaga ctgcgccctc ggattcatga tcatgcaggc agtggcaaag gttatttctt
      961 ctcgcttgaa ccatacacgc atcatcttag tgggcgaaag aggattgtcg gttttaaccg
     1021 agtattagtt gttaaaatgc ggattggagg ggaaaatatg gctgatcaaa taataatata
     1081 tggcaaagtt ggttgaccct acacagacaa agccagatcg gctttcggcg acaaagctat
     1141 ttatttagat gtaacggcca acacagacca attggcggaa atgttaaaaa tatccggggg
     1201 aacacgtcag gtgcccgtta ttgttgaaaa tggcaaagta accattggtt acggcggtac
     1261 ctgaggagtt tgatgaccgg tcgggaaccg gttttaaaat tgataataag taaggccaac
     1321 catgaaacat ccaaacctcg atgccattat tgaaaaagcg attgccaatg aagaagaagc
     1381 aaatcagttt tacctgaagc tttcggaaat cgttaccgat aaatcggcta aagacactct
     1441 actttatctg gccaaagagg aaaaaaagca taaagaatat ttgttacact accgtcagga
     1501 aagttttatc ggcaatgcca ctaaatccag tgaaatgacc aactataaaa tagcggaatt
     1561 ccttgagccg cccgctgtcc aattcaatat ggatagcaaa gatgtatatc ttgttgccgc
     1621 ggaaagagaa cttaactctt ataattttta tatggggctg gcagccattc atcctgacgg
     1681 agaaataaaa gatatgctga tgaaaatggc ggctcaagag ttgaagcaca aggaaaaggt
     1741 ggagtatcta tactcgaata cagcttttgt tcaaaccgcc gggggatagt ctataaccgg
     1801 acttgttaaa aaataaattc ttttgccgct tacggcaaaa aaaggagttg aaaagtggat
     1861 gattttgtac ccagaagaat tttt
//
LOCUS       DASVIM010000041         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1637484, whole genome shotgun sequence.
ACCESSION   DASVIM010000041 DASVIM010000000
VERSION     DASVIM010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637484"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..98
                     /gene="rpsK"
                     /locus_tag="VF305_00795"
     CDS             <1..98
                     /gene="rpsK"
                     /locus_tag="VF305_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX7415702.1"
                     /translation="SLQLAGLTITVIRDVTPVPHNGCRPPKRRRV"
     gene            128..757
                     /gene="rpsD"
                     /locus_tag="VF305_00800"
     CDS             128..757
                     /gene="rpsD"
                     /locus_tag="VF305_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX7415703.1"
                     /translation="MARYIDSSCRLCRREGLKLFLKGDRCYSEKCAFERRGYAPGDHG
                     QAHKKQHSDYGVQLREKQKLKRMYGLLEKQFRGYFEKADQQKGITGTNLLILLERRLD
                     NMIFRMGFANSRVEARQLISHSHFLVNGKSVNIPSYLLKAGDGVCVKEGSRKITRIFG
                     AMEIVARRGVPHWLELDKDNFKASIKMLPVREDLTMPVQEQLIVELYSK"
     gene            803..1792
                     /locus_tag="VF305_00805"
     CDS             803..1792
                     /locus_tag="VF305_00805"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX7415704.1"
                     /translation="MQRNWSSLIRPKRIDVDEATHTHFYGEFTIQPLERGFGITLGNA
                     LRRVLLSSIQGTAIASVKIDGVLHEFSTVPGVKEDVTDIVLNLKGVRFKLGQADTKEV
                     HINVARTGAITAADIITDGTIEVLNPDHYIATVSDKGKFKAELVVKMGKGYIPAKKEL
                     EPDQPEGTINIDAIFSPIKKVNYVVSHARVGQIADYDKLVLEVWTDGSVSPEDAIAYC
                     AKILKQQLDVFINFEEIEEEIPPEKEDEKGDVNKVLLRSVEDLELSVRSANCLKNAGI
                     NTIGELVEKTEAEMLKTKNFGRKSLSEIKDILGEYGLTFGMGLDFAPWNEKNQ"
     gene            1851..2198
                     /gene="rplQ"
                     /locus_tag="VF305_00810"
                     /pseudo
     CDS             1851..2198
                     /gene="rplQ"
                     /locus_tag="VF305_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            2588..>2792
                     /locus_tag="VF305_00815"
     CDS             2588..>2792
                     /locus_tag="VF305_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415705.1"
                     /translation="MAEKKIGEVVKFFSNPSVAAVKITEGELVVGDSIKFSGHTTDFI
                     DMIKSMEVDNKSVQKAVAGDFIGV"
BASE COUNT          904 a          449 c          683 g          756 t
ORIGIN      
        1 gatcgttgca gcttgccggg cttactatta ctgtgattcg tgacgtaacg cctgttccgc
       61 ataacggatg cagaccacca aagcgtcgta gagtataatt aataattaga aaatcaggga
      121 ggcttttttg gcaagatata tagattcctc atgtaggtta tgtcgccgtg aaggattgaa
      181 actttttctt aaaggcgata ggtgctattc ggaaaaatgc gcttttgaaa gaagggggta
      241 tgctccggga gatcatggtc aggcgcataa gaaacagcat tctgattatg gtgtccaatt
      301 gcgggaaaag caaaagctca aaagaatgta tgggcttttg gaaaagcagt ttagaggcta
      361 ttttgaaaag gccgatcagc aaaagggtat tacagggact aatttgctta tacttctgga
      421 aagaagattg gacaatatga ttttccgcat gggttttgct aattccagag tggaggcaag
      481 gcaattaatt agccatagtc attttttagt caatggcaaa tcggttaata tcccatcata
      541 tctgcttaaa gccggagatg gagtttgcgt taaggaaggc agccgtaaga taactcgaat
      601 atttggagca atggaaattg tagccagacg tggcgtgcct cattggctgg aattggataa
      661 agataacttc aaagcctcca ttaaaatgct gccggttcgg gaagatttaa caatgccggt
      721 gcaggaacag cttattgtag agctttattc gaagtagaaa aacaaattat tttcataaac
      781 cagctaaagg atgggtttga ttatgcagag gaactggtca agtttgattc gtcccaaacg
      841 tattgatgtt gatgaagcga cgcatacgca tttctatgga gaatttacta ttcagccttt
      901 ggaaaggggc tttggcataa ccttaggcaa tgcgttaagg agagtgttgc tttcttctat
      961 tcaaggtact gctattgctt cagtaaagat tgatggcgta ttgcacgaat tttcaactgt
     1021 acccggagtt aaagaagacg taaccgacat tgttttgaat ttaaagggcg ttcgctttaa
     1081 attgggacaa gcggacacaa aagaagtgca catcaatgtg gctcgtaccg gagctattac
     1141 agcagccgat attattaccg acggcacgat cgaggtttta aaccccgatc attatattgc
     1201 cactgtatcc gataaaggaa aatttaaggc cgaactggta gtgaaaatgg gcaagggata
     1261 cattccggca aaaaaggaac ttgaaccgga tcaacctgaa gggacgatta atattgacgc
     1321 tattttttca ccgattaaaa aagtgaatta tgttgtttct catgcgcgcg tcggtcaaat
     1381 tgccgattat gataaacttg tgctcgaagt ttggacggat ggcagtgtga gtcctgaaga
     1441 tgccattgcg tattgtgcaa aaatactgaa acaacaattg gacgtattta ttaattttga
     1501 agaaattgag gaagaaattc cgccggaaaa agaggatgaa aaaggagatg taaataaagt
     1561 cttgctacgt tcagtagaag acttggaact ttctgtccga tcggctaact gtttgaagaa
     1621 tgccggaatc aacactattg gggaattagt ggaaaagaca gaagcggaga tgctcaaaac
     1681 aaagaatttt ggccgtaaat cactaagtga aatcaaagat attcttggtg aatatggtct
     1741 tacttttgga atgggtcttg atttcgcacc gtggaacgaa aaaaaccagt agtatattag
     1801 caaatgatct gtctttcgga tttgattgtt aagaaaggaa actattagca atgtatcatg
     1861 gtaaagcggg caggaagcta ggcaggactt ccagccataa ggaagccatg tttcgtaata
     1921 tggtaacttc cgttataaaa catgagcgta ttaggacgac cgactgcaaa gcaaaagagt
     1981 taagaaagct ggcggaaaag ctgattaccc tgggcaaaaa agggagtttg cacgcgcgcc
     2041 gtcaggcttt agctgttgtc cgggataaat ctttagtggg taaactcttt ggggaactca
     2101 ccgaacgcta ccgtgaccgt gcaggcggat acacgaggat aattaaggcc ggttatcgct
     2161 ttggtgataa tgcgcctgtt tccattttgg aatttattcc tgatgggaaa aagaaggaaa
     2221 aagcaaaacc taaggcaaaa ggtaaagcaa aagaaaaaga ggaagagaaa tcagctttgg
     2281 aagctattcc tgaggaaaaa aagaaagaaa aagcaaaagt taaggtcaaa agtaaagcga
     2341 agaaggaaga gaaagaaaaa tcagctgaat agataattct acattagaaa acaataaaaa
     2401 aggcagggtt ttgaccctgc cttttatatt gttttttaac actaaaaaat gtatttagtc
     2461 cccagaggtg tgcttgctca gttgtatgaa agccaacttg ttaccggttt gtatcttgtc
     2521 caatgcaacc gataattatt ttgttgaggc attaatttgc aaacataata atggtggagg
     2581 tttggttatg gcagaaaaga aaattggcga agtagtaaaa tttttctcaa acccttcggt
     2641 agctgctgtt aaaattactg aaggtgaatt ggttgtgggc gacagcataa agttttccgg
     2701 ccacacgact gattttattg atatgatcaa gtctatggag gttgacaaca aatcagtaca
     2761 aaaggctgta gccggtgatt tcatcggtgt ta
//
LOCUS       DASVIM010000042         4157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1126073, whole genome shotgun sequence.
ACCESSION   DASVIM010000042 DASVIM010000000
VERSION     DASVIM010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126073"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VF305_00820"
     CDS             complement(<1..296)
                     /locus_tag="VF305_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7415706.1"
                     /translation="MIYFRNVSLSFLDKKLFDNINWTIHPKNRIGLVGDNGTGKTTLF
                     RAILAQVHLDSGAIDIPNRKQKTIGYLPQDLVELESIALIEFLKRKSGIATLEES"
     gene            complement(390..1709)
                     /gene="der"
                     /locus_tag="VF305_00825"
     CDS             complement(390..1709)
                     /gene="der"
                     /locus_tag="VF305_00825"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX7415707.1"
                     /translation="MQAKPLIAIVGRPNVGKSTLFNRIAGRQKAIVIDEPGATRDRNY
                     MDCVWDDKAFTLIDTGGFEPASEERILVQMREQTNLAIEEADVIIFLMDGKDGLTPSD
                     IEIAKQLRGKDKNVFYVANKIDGDRHKEMLGEFYKLGIDQFYPISAQHGLGVDELLVT
                     VTHDFPSITDAKDKQDDRIKIAIVGKPNVGKSSLVNRILGKERSITNPIPGTTRDAID
                     MTVKVRGKSYLLIDTAGIRKKSKISLTLEKYSVVQALKSINRCDIALVLIDAEEGLTE
                     QDTKIIGLAYERGKACIIVVNKWDKIEKDNSTVGKFVDDIKDKIKFMDFAPIIFISAV
                     TGQRAPKIFDLIDSVYSQYTKRIGTSPLNDLVEKSVQKNSMARYANRQMHISYATQTD
                     VKPPTFVFFVSNTKGIHFSYERYLMNQIRGTFGFDSVPLKIIFRKKK"
     gene            complement(1737..2627)
                     /gene="era"
                     /locus_tag="VF305_00830"
     CDS             complement(1737..2627)
                     /gene="era"
                     /locus_tag="VF305_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX7415708.1"
                     /translation="MFKSGFIGIVGRPNAGKSTLFNAIIGDNISIVANKPQTTRNKIT
                     GIKNLPDAQLIFLDTPGMHKPKTPLNRAMIQTAQETIGDADVLLMLIEANSAVHPHDL
                     FLIESLAQIRVPVFLVINKIDLIEKKFLLPLIDKFRTLYDYREIFPISALKGDGLAAL
                     LEAIKELLPEGPQLFPEDILTDATERFIAAEFIREKIILLTKQEIPYASAVTIDLFQE
                     DKGKNLIRISATISVEKESQKAIMIGKKGAMLKNIGIQARLQMEKFFGTRVYLELFVR
                     VKKDWTSSDKMLQEFGLLKH"
     gene            complement(2791..3882)
                     /locus_tag="VF305_00835"
     CDS             complement(2791..3882)
                     /locus_tag="VF305_00835"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7415709.1"
                     /translation="MNEINNEVKNNSKPLIIPIFIMNSGCPHRCIFCNQQITAGNFPK
                     ELTKEFFDAEVKSYLGWNKEKSRNVEIAFYGGSFTGVAPAYQEKLLSWAYSFIQTELV
                     NSVRISTRPDYIKEDNLSLLKKYGVATVEIGAQSFIDNVLQFAQRGHDAASILKAIGI
                     LKDHGFRTGLHLMAGLPKDTKERFLYSVEKTIELKPDTVRIHPVVVFSGTALAKELRQ
                     GKYKPLLLSEAIDLCRLAWKKLSTAGIRIIRIGLHWTKEMEKNGAVLAGPIHPALGNL
                     VLSSIFYDQTIKLMDNIHRSAKELRFNLSYQDISNFRGLNNTNVSAIKKLYPHTKIIV
                     VYSSEQKRGAISIDTDSGEFFTTEISGII"
     gene            complement(3875..>4157)
                     /locus_tag="VF305_00840"
     CDS             complement(3875..>4157)
                     /locus_tag="VF305_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HEX7415710.1"
                     /translation="KNAVNKLIEPLLKDDSITFKYFDYKTALQELCQKRYKTAPVYTI
                     IDSCGPDHAKTFAVKVTIADKTTQVGSGKSKKEAEKQAAQKAWEILQDE"
BASE COUNT         1084 a          870 c          887 g         1316 t
ORIGIN      
        1 gattcttcca gagtggcaat cccgcttttt ctcttgagaa actcgatcag ggcgatagat
       61 tcaagttcca ccagatcttg cggcagatag ccgatcgtct tttgtttcct gttgggaata
      121 tcgattgcgc ctgagtcgag atgaacttgc gcaagtattg ctcgaaatag agtcgtttta
      181 cccgtaccgt tatcgccgac aaggccgatc cggtttttgg gatgaattgt ccagttgatg
      241 ttatcgaaca gctttttatc caaaaaagac aagctgacgt ttctgaagta aatcaattac
      301 gcccccaatt tatataccag agcgtatata ggaaaaatga gtcaaactgt caatttttaa
      361 tatccaatca aggaaagggt tagtaaggat tatttctttt tccggaatat aatttttaat
      421 ggcacactat caaaaccaaa agttccacgt atttgattca tcaagtaacg ctcgtaggaa
      481 aaatggatac ctttggtgtt gctgacaaag aagacgaaag taggcggttt gacatcagtc
      541 tgggtggcat aagaaatatg catctgacgg ttggcataac gcgccataga gtttttctgc
      601 acagattttt ccaccagatc atttaatggg gatgtgccga ttctctttgt atactggcta
      661 taaactgagt cgatcagatc aaaaattttc ggcgcccgct gccctgtaac tgccgagata
      721 aaaataatgg gcgcaaagtc catgaatttg attttatcct taatatcgtc gacaaatttc
      781 cccactgtgg agttatcctt ttcaatctta tcccatttgt tgactacgat gatgcaggct
      841 ttgccgcgct cataggcgag cccgataatc tttgtgtcct gctcggtcag cccctcttcg
      901 gcatcaatca aaacaagcgc gatatcacag cgattgattg atttgagtgc ctgaacgacg
      961 ctatattttt ccaaagtcaa gctgattttg cttttcttgc ggattcccgc tgtgtcgatc
     1021 agcagataac ttttgccgcg cacctttacg gtcatatcaa tggcatctct tgttgtgcct
     1081 gggatcggat tggtaatgct tctttccttg ccgaggatac ggttgacgag tgatgatttg
     1141 ccgacgttgg gtttgccgac aatggcaatt tttattcgat cgtcttgctt atccttggcg
     1201 tctgttattg aggggaaatc atgtgtaaca gtaaccaata gttcatcaac gcccaaacca
     1261 tgctgtgctg agatcggata gaattggtcg atcccgagct tataaaattc gcccagcatt
     1321 tctttgtggc ggtctccatc tattttattg gcaacgtaaa aaacattctt atctttaccg
     1381 cgcagttgct tggcgatttc aatgtctgag ggtgttagcc catcttttcc atccattaga
     1441 aagatgatga catctgcttc ttcgatggcc agattggtct gctcgcgcat ttgcacgagg
     1501 attctttctt cagaagctgg ttcaaatcca ccggtgtcaa tgagcgtaaa agctttatcg
     1561 tcccagacgc agtccatata gttacggtcg cgcgtagcgc ccggctcgtc aataactata
     1621 gctttttgcc ttccggcaat tctattaaaa agagtcgatt tgccgacatt gggcctgccg
     1681 acgatggcga ttagaggttt tgcttgcatc taagtattat agcctcttta aaaagtttag
     1741 tgttttaata aaccaaattc ttgcagcatt ttatcggagc ttgtccaatc cttcttaacc
     1801 cgaacaaaga gttccagata aaccctggtg ccgaaaaatt tttccatctg gagcctcgcc
     1861 tgaataccaa tatttttaag catggcgcct tttttcccga tcatgattgc cttttgtgat
     1921 tctttttcga cactgatcgt cgcactgata cgaatgaggt tttttccctt atcctcctgg
     1981 aataagtcaa tggtaacagc cgaggcgtaa ggaatttctt gcttggtgag cagtataatt
     2041 ttttcgcgga taaattcggc cgctataaac ctctcggttg catcggttag aatatcttcc
     2101 ggaaacaatt gtggcccttc agggagtagt tctttgatag cttctagtaa agctgcaagg
     2161 ccatcgccct ttaatgcaga aataggaaaa atttcccgat agtcataaag tgtgcggaat
     2221 ttatcaataa gcggcaaaag aaactttttt tcgataaggt caattttgtt gatgactaaa
     2281 aaaacaggaa ctctaatttg cgccaatgat tcaattaaga aaagatcatg gggatgaaca
     2341 gcggaattgg cttcaataag catgagtaaa acgtcggcgt cgccgattgt ttcctgtgca
     2401 gtttgaatca tagcgcgatt cagcggagtt ttgggcttgt gcattccggg agtgtccaga
     2461 aagatcagtt gtgcatcggg taggttttta atgccggtaa ttttgtttct tgttgtctga
     2521 ggtttattgg caacaatgga aatattatcg ccgataatag cgttgaataa cgtggattta
     2581 cctgcgttcg ggcggccaac gatgccgatg aaaccggatt tgaacacgtc attaatctcc
     2641 tgtaattcca accctaaatc aaagcgctat ttttgttggc gtcgttgtcg gcttcctcaa
     2701 cgtatgtaca acgcgtgacc tttaggttgt acgcctcgtc gcctccgcct agccgcctca
     2761 atatcatctt tgatttagga ttggcataag ttaaataatt cccgaaattt ctgtggtaaa
     2821 aaattctccc gaatcggtgt ctatggaaat tgcaccacgt ttttgttcac tggagtatac
     2881 tacaattatc tttgtgtggg ggtaaagctt ttttattgcc gatacgtttg tgttgttcaa
     2941 accgcgaaaa ttagagatat cctgataaga caagttaaag cgcagttcct ttgcacttcg
     3001 gtgtatatta tccatgagtt ttattgtttg atcataaaaa atagacgaaa gaacaaggtt
     3061 tcctaaagcc ggatgaattg gtccggctaa taccgcgcca tttttttcca tttccttagt
     3121 ccaatgcaag ccaattctga tgatacggat tcccgctgtg gataactttt tccaggccag
     3181 ccggcataag tctatggctt cagagagtaa taagggcttg tatttcccct gccggagttc
     3241 tttggcaagg gcagtcccgc taaaaacaac gaccggatga atgcgcaccg tatcaggctt
     3301 gagttcaatg gtcttttcta cagaatataa aaatctttct ttcgtatctt taggcagacc
     3361 ggccatcaag tgtaagccgg tacgaaagcc gtggtctttt aaaattccta tggcctttaa
     3421 aatggaggcg gcatcatggc ctctttgcgc aaattgcaga acattatcga tgaaagattg
     3481 cgcgccaatt tccactgtag cgacgccgta ttttttcagg agagataaat tatcttcttt
     3541 gatgtaatcc ggtcttgtcg aaattctgac agaattaaca agctcagttt gaataaaaga
     3601 gtaggcccaa gaaagaagct tttcctgata agcgggggca actcccgtaa aactgccgcc
     3661 gtaaaacgca atttccacat ttctggattt ttctttattc cagcccagat atgacttaac
     3721 ttcagcatca aaaaattctt ttgttaattc tttcgggaaa tttccggctg taatttgctg
     3781 attgcagaag atacaacgat gcgggcaccc gctattcatg atgaaaatag gaattatcaa
     3841 aggtttactg ttatttttaa cttcattatt gatttcattc atcttgcaga atttcccagg
     3901 ccttttgtgc tgcctgtttt tcggcttcct tcttactttt accgctgccg acttgcgttg
     3961 ttttatccgc aattgttacc ttgacggcga aagttttggc atgatccgga ccacaggaat
     4021 caatgattgt gtaaactggc gctgttttat atcttttctg gcaaagctcc tgcagagcag
     4081 ttttataatc aaaatattta aaggttatgc tatcgtcttt caacaaaggt tcaattaatt
     4141 tatttacagc attttta
//
LOCUS       DASVIM010000043         4764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_444301, whole genome shotgun sequence.
ACCESSION   DASVIM010000043 DASVIM010000000
VERSION     DASVIM010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444301"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            623..853
                     /locus_tag="VF305_00845"
     CDS             623..853
                     /locus_tag="VF305_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415711.1"
                     /translation="MEKNYKGFCTSRPFWAGEKPSFSILNRSEHFAKIMEEEIFSYES
                     EVYRLKICKDGMILFRNEELEKNIKKEYEISR"
     gene            1186..4734
                     /gene="smc"
                     /locus_tag="VF305_00850"
     CDS             1186..4734
                     /gene="smc"
                     /locus_tag="VF305_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX7415712.1"
                     /translation="MKLKRLEISGFKSFRDKVVFDFPDGITAIVGPNGCGKSNVVDSI
                     RWVMGEQRVKALRGKRMDDVVFNGSQDSSAVSMAEVTMTLAANAHGFPGAYAEMGEVS
                     ISRKVIRDGDSEYYLNRVPCRLLDVKEFFMGTGVGTRTYSLVEQGNVASLVEAKPEDR
                     RLFIEDAAGVSKYKSRKDAAVRKMEATKQNILRLNDIIKEVKSQLNAISRQAKRAEQY
                     KEIKQGMKETELTIALQSFTELAEKSSSLQARRAELQNRDAGMRTNLEAKESALEELK
                     TKLLENDELIAKSQQELYETKNSISIKEKNIEFARRQMADTSERKQKDLMEIQLLRTK
                     KSDLAAEIENLQKSAAETESKIADLKIAHDDMQQKVQELINADKEINNQLEEKKISYI
                     DVVTEKAKLKNMISSLGKSIEDLNKREEKENHEIDEDKKMLADLTSKLQSVIKGLSNN
                     EEEFTRLAEKRETASAEVDKDKSDLQLSEESVAKIKEETSLKSARLSSLKEFQEAYKW
                     SNEGVKAIIENKESRDKFNGVVADHINVPRKYEAAVEAVLGEKLQYVVVKSQEDGVWA
                     IDHLKSYQLGRGSFVPVDLRSHNTENYNTDHFPEAESLLSKVNVKENFKQIADCLLGD
                     VLLIPSIASGISLWKKNGFRGTFVTPEGDIISPHGVLTGGSGAAVEKSLLATKREIAE
                     LESEVSALVSELETQTSQRKKLVSVIAQWEEEIGQVKTGIHSLEIDINGKKKDRERFE
                     DEVTRLKQRIAALEFNRQNIKAEEAEAAEKLQKFNTDILRKDEEEKEINAIISNFNVK
                     REQSRAAIDEQERHLTGKKIEMASLEEKKEADLRTISRLQNDISAIANDDRIKEEEIV
                     SCEKQLTELARTIEEEQTALKELYQGFAVQETALAEKKARQNREDEQVKAKENEIREI
                     KKKLDELREKINEMEIQCREATLNSENLKRAVAEKHSVDLENMVPGFTKIEEEKLTEL
                     IALLEKNKQTIDNFGEVNLLALNEYEELDKRYQFLSAQISDLNASLNALQRTITRINK
                     ISRTRFAETFAAVNACFKEVFTHIFPGGRGQLLLTDENDLLETGVDIDIQIPGKKAQS
                     VNLLSGGEKSLAAVALIFAILMYRPSPFLVLDEVDAALDDANTNLFNRLIKDVAKKSQ
                     ILMITHNKSTMEVADTLFGVTMQKLGISSLVAVNLN"
BASE COUNT         1627 a          876 c         1056 g         1205 t
ORIGIN      
        1 aagacctaca atataccaga caatttgggt gcctcggtat aacggcttag tagtgcgaga
       61 gttgtatgag tccatatttt ttatacctat taattttttt gagtttaata cttggttacg
      121 accgccaccg catatctatt aaatataatt taatcttaac atattaatca ttaagttgaa
      181 agtaaatttt ctccgctcaa ttaaaccaag gataataact cctttatctg ccttatcgcc
      241 atatccgggg ctggaaacgc ttttttccaa gcataagttc gaaagttacg ccatttcttt
      301 aattcttctg ttgtcatgcg tataatatta gccattggct tattattttc aagagggaaa
      361 ataataaaat tgaagattca tccggttgat acgtgcttta tgaaagcaac aagccgtcca
      421 cctcgatgat gatgcatgat tccaaggcat aatcattgat gttatttact tccgatgcca
      481 accgtaagga tcatcaggtg acctacacct tacattgaca cacgaaagca catacagtat
      541 gcttctctcg tcacccaaaa atgtcttgtt cctgcaatat aggtaattta ctgaatcata
      601 ataaatattc ggagtgtcat tgatggaaaa gaattataaa ggattctgta cttcaaggcc
      661 cttctgggcg ggagaaaaac catcgttttc gatattaaat cgttcagaac atttcgcgaa
      721 gataatggaa gaagaaattt tttcgtatga atccgaagta tatcgattaa aaatatgcaa
      781 ggatgggatg atacttttta gaaatgaaga gctggagaaa aatataaaga aggaatatga
      841 aataagtagg tgaaatagag cagggttcac cggcaaataa atattttatt tgcattacac
      901 tctcctttat gcgatggact tccgaaaata acatatagct taaaccacca tgttcgtcgt
      961 aattttgacc tgtttttgac taaaatagac actatcaggc aggcgcctgt ctataaaatt
     1021 gtcccgggaa caagaagctc taaggaaaaa gtcaaaaaag atcaaagcac tatatattgt
     1081 ggtattaata tttcattacc acattttgtt gtattttttc tttacaaagg aaggttcttt
     1141 tgttaggatt ttgcaaaatt taagcttgtg cgttaaagaa aattcatgaa attaaaaaga
     1201 ttagaaatat ccggttttaa atcatttcgc gacaaggttg tctttgattt tcccgacggc
     1261 attaccgcga tagttggacc caacggctgt ggaaaatcca atgtagttga ctccatacgc
     1321 tgggtaatgg gagagcagcg ggtcaaagct ttgcgtggaa aaagaatgga tgatgtcgtt
     1381 ttcaacggca gtcaagactc ctcggcggta agtatggccg aggttactat gactttggcc
     1441 gccaatgctc atggcttccc gggcgcttat gccgagatgg gggaagtatc catttcccgt
     1501 aaagttatcc gtgacggcga tagtgaatac tatctcaatc gggttccctg ccgccttctt
     1561 gatgtcaaag aattcttcat gggtaccgga gttggtacca gaacttattc tctggtcgaa
     1621 caggggaatg tggccagcct tgtcgaagct aaaccggagg atagaagatt gtttattgag
     1681 gatgctgcag gcgtatccaa atacaaaagc cgcaaggatg cggcagtccg taaaatggaa
     1741 gcaaccaagc agaatatact tcggctcaat gatatcatta aggaagtaaa atcccaactc
     1801 aatgccattt cccggcaggc taagagagct gagcaataca aggaaattaa gcaaggcatg
     1861 aaagaaacgg aattgacgat tgccctgcaa agctttaccg aacttgctga aaaatcgtct
     1921 tcgttacaag cccggcgtgc agaactgcaa aaccgggatg ccggcatgcg tacaaatctc
     1981 gaagctaaag aatcagcttt ggaagagcta aaaacgaagc ttttggaaaa tgatgagtta
     2041 atagctaaaa gccaacaaga gctttatgaa actaaaaact ctatcagtat taaagaaaaa
     2101 aatatcgaat ttgcccgccg ccaaatggcc gacacttccg aacgcaagca aaaagactta
     2161 atggaaattc agttactgcg gacgaagaaa tcggatttgg ctgctgaaat tgaaaatctg
     2221 caaaaaagtg cagccgaaac cgaaagcaag attgctgatc tcaaaattgc acatgacgac
     2281 atgcaacaaa aggttcagga gctgatcaat gccgataaag aaataaacaa tcagctggaa
     2341 gaaaagaaaa tatcatatat cgatgttgta acagaaaagg ccaagctcaa aaatatgatt
     2401 tccagcctgg gcaaaagtat cgaggattta aataaacgtg aagaaaaaga aaatcacgaa
     2461 attgatgaag ataaaaagat gctggcggat ttaacaagca agctccaatc agtaattaaa
     2521 ggattatcga ataacgaaga agaattcacc cgattggcag aaaaaagaga gactgcatca
     2581 gccgaagtcg ataaagataa atcagattta cagttaagcg aggagagcgt cgctaaaatt
     2641 aaggaagaaa ccagcctgaa gtcagcccgt ttaagttccc tgaaagaatt tcaagaagca
     2701 tacaagtgga gtaatgaagg cgttaaggca atcatcgaaa acaaggaaag ccgtgataag
     2761 tttaatggag ttgtggctga ccatatcaat gttccccgta aatacgaagc agctgtagaa
     2821 gcagtgttgg gagaaaagct gcagtatgtt gtcgtaaaaa gtcaggaaga cggcgtgtgg
     2881 gcaattgatc atttgaaaag ctaccagctg ggcaggggca gttttgtgcc tgtagatttg
     2941 agaagtcata atacagaaaa ttacaacacc gaccatttcc cggaagcgga gtccttactt
     3001 tcaaaagtta atgtcaaaga aaattttaag caaattgccg attgtctgtt aggtgacgtt
     3061 ttattgatac catcaattgc aagcggtatc tctctgtgga agaaaaatgg tttccgcggc
     3121 acctttgtta cgccggaggg agatattatc agcccgcatg gagtattaac cggcggcagc
     3181 ggagccgcag tggaaaaaag tcttctggca acaaaaagag aaattgccga gttggaaagt
     3241 gaagtgtcag cactcgtttc agaattggaa acgcaaacca gtcaaagaaa aaaacttgtt
     3301 tccgtaatcg ctcagtggga agaagaaatt ggtcaagtaa aaactggaat ccactctctg
     3361 gaaatcgata ttaacggcaa gaaaaaagac cgcgaacggt tcgaagacga ggtaaccaga
     3421 ttaaaacaga gaattgctgc tttggagttc aatcggcaaa atattaaggc ggaagaagct
     3481 gaagccgcgg aaaagttgca aaaatttaac accgatattt tacgcaaaga tgaagaagaa
     3541 aaagagataa acgcgattat ctctaatttt aacgtgaagc gcgaacaatc tcgcgcggca
     3601 atcgatgaac aagagcgcca tttaacgggc aaaaaaattg aaatggcttc tttagaagag
     3661 aaaaaggaag ccgatctgcg gacaatatcg cggctgcaaa atgatattag cgctattgcg
     3721 aatgatgacc ggattaaaga ggaggaaatt gtttcttgcg aaaagcagct taccgaatta
     3781 gctcggacaa tcgaagagga gcaaacggcg ttaaaggaat tatatcaggg cttcgcggtg
     3841 caggaaacgg ccttggcgga gaaaaaagca agacaaaatc gagaagatga gcaagtgaaa
     3901 gcaaaagaaa atgaaattcg ggaaataaaa aagaagctcg atgaattacg cgagaaaata
     3961 aatgaaatgg aaattcagtg ccgggaagca accttaaata gcgaaaattt gaaaagagca
     4021 gtcgccgaaa aacacagtgt tgacttggaa aatatggtcc ccggatttac gaaaattgag
     4081 gaagagaagc tcaccgaact catcgcctta ctggaaaaga ataaacaaac aatagataat
     4141 tttggcgaag taaatttgct tgctctcaat gaatatgaag aactggataa gcgttaccaa
     4201 tttttatcgg cacaaatatc agatttaaat gcttcactga atgctttgca aaggacaatt
     4261 acacgcatta ataaaatttc ccgcacgaga tttgccgaaa cctttgccgc cgtcaatgcc
     4321 tgttttaagg aagtattcac tcacattttc ccggggggcc gtggacagct tttgctaacc
     4381 gatgaaaacg atttactgga aacaggcgtg gatattgata ttcaaatccc cggcaaaaaa
     4441 gcgcaaagtg tcaatttgct ttccggcggt gaaaaatcgt tggcggccgt tgcccttatt
     4501 tttgccattt tgatgtaccg tccttcgcct ttcctggttt tagacgaagt tgatgccgct
     4561 ttagatgatg ccaataccaa cctctttaac cggttgatca aagatgttgc caagaaatct
     4621 cagattctca tgattacgca taacaagagc acaatggaag ttgccgatac gctctttggc
     4681 gtgacgatgc aaaaactggg catatcttct ttagtcgcag tcaatcttaa ttaatccatt
     4741 cctcaagttt tgcgcgcctt gcct
//
LOCUS       DASVIM010000044         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_956710, whole genome shotgun sequence.
ACCESSION   DASVIM010000044 DASVIM010000000
VERSION     DASVIM010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956710"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="VF305_00855"
     CDS             <1..1413
                     /locus_tag="VF305_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7415713.1"
                     /translation="DFVREEDRTEIADTLQKIFTDKSTIRDKNFIFLNKKRLPRYVTL
                     TGTPDFDAEGKVIGVVGTLKDITMLRSMEAQLLQASKMEAVGTLTGGIAHDFNNIIQA
                     IMGYNQLMISGRADDETDMSYLQSIGELIQRSRELVRQLLIFSKKVEPLSKVVNVNYE
                     IKNMYSLLTKSIPKMIEIQTYLDEDISPVNADSTQIGQIIMNLVINARDAMGDSGNII
                     IKTNNLTLPENTSIAGLKIPAGNYVDLTVADTGCGMEKELMQHIFEPFFTTKEPGKGT
                     GLGLAVVHGIVKNHGGFIYCESKPDKGTTFKILFPATTAVKPQQKVQEKTAKIPRGKE
                     KLLIVDDEKNILETVKETLSSYGYKVMTAESAEQALEVYTAQKDKIKMVLLDLNMPGK
                     GGKKCLIDLLTINERAKVLMTSGYSGPQEIEEITNAGAAGFINKPYRSEDLLRSIRKI
                     LDAPHQPNKGRIPLQSNKGV"
     gene            complement(1365..>1763)
                     /locus_tag="VF305_00860"
     CDS             complement(1365..>1763)
                     /locus_tag="VF305_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415714.1"
                     /translation="LLDLDYNCLSHYELRAPMAAILPKLMNFMDEEYNFDTFVGRKLY
                     SFLYDNGFENIDVELMAHNLIFGEVKDKDKFNWNKKVEMGARKAEKLLNSYPGGYQQF
                     SSDLNKFLVDPRRFTYTPLLLCKGIRPLLG"
BASE COUNT          592 a          360 c          344 g          467 t
ORIGIN      
        1 gatttcgtac gggaagaaga ccggacagaa attgccgata ctttgcaaaa aatttttaca
       61 gacaaatcaa ccattagaga caaaaacttt atttttttga acaaaaaaag gttaccccgt
      121 tatgtcactt tgaccggaac tccagatttc gatgcggaag gaaaagttat aggcgtcgtg
      181 ggcactctca aagacatcac catgttgcgc agtatggaag ctcaattgct gcaagcctcc
      241 aaaatggaag cagtcggcac tttaaccggc ggaatcgctc acgatttcaa taatattatt
      301 caagcaatta tgggctacaa ccaattaatg atttcgggaa gagccgacga tgaaacggat
      361 atgtcttatc ttcaaagcat cggagagcta attcagcgct ccagagagct tgtcaggcag
      421 ttgcttattt ttagcaaaaa agtggaaccg ctatcgaaag ttgttaatgt taattatgaa
      481 attaaaaaca tgtatagttt gcttacaaaa tccattccca agatgattga aatacaaacg
      541 taccttgatg aagatatttc ccccgttaac gccgattcca cccagatagg acaaattatc
      601 atgaatctgg tcattaatgc ccgcgatgct atgggcgata gtggaaatat aataataaaa
      661 acaaataacc ttaccttgcc ggaaaatact tcgatagctg ggttaaaaat tcccgccggt
      721 aattacgttg acctgaccgt tgccgatacg ggctgcggca tggaaaaaga attaatgcaa
      781 cacatctttg agcccttttt taccactaag gaacccggga aaggcaccgg cctgggactt
      841 gccgttgttc atggaattgt gaaaaaccat ggtggcttta tttactgtga aagcaaaccg
      901 gacaaaggaa caacttttaa aattttattc cccgccacaa cagcagttaa accgcaacaa
      961 aaagttcagg aaaaaaccgc gaaaattcct cgcggcaagg agaaattact tatagttgat
     1021 gatgaaaaaa acattttaga aacagtcaaa gaaacattga gttcttacgg ttataaagta
     1081 atgactgctg aaagcgccga gcaggcgcta gaggtttata ccgctcaaaa agataaaatc
     1141 aagatggtgc ttctcgattt gaatatgccc ggcaagggcg ggaaaaaatg ccttattgat
     1201 ttgttgacca ttaatgaaag ggctaaagtt ttaatgacca gcggctattc tggtccccag
     1261 gaaatagaag aaataaccaa tgccggagcg gcgggattta ttaacaaacc ttaccgttcg
     1321 gaagatttgc tacgcagtat tcggaaaatc ctcgatgccc cgcatcagcc caacaaaggc
     1381 cgaattcctt tgcaaagcaa caaaggagta taagtaaaac gtcttggatc aactaaaaat
     1441 ttattcaaat cactggaaaa ttgttgatac cctccgggat atgaatttaa taatttttct
     1501 gcttttcttg cccccatttc aactttctta ttccaattaa atttatcttt atcttttact
     1561 tcaccaaaaa ttaaattatg cgccatcaat tccacatcga tattttcaaa accgttgtca
     1621 taaagaaatg aatagagttt gcgtcctaca aatgtatcaa aattatattc ctcatccatg
     1681 aagttcataa gcttgggcaa tatcgcagcc attggcgccc gcagctcata gtggctcaaa
     1741 cagttataat ccaaatcgag cag
//
LOCUS       DASVIM010000045         4483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_529478, whole genome shotgun sequence.
ACCESSION   DASVIM010000045 DASVIM010000000
VERSION     DASVIM010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529478"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VF305_00865"
     CDS             <1..1044
                     /locus_tag="VF305_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEX7415715.1"
                     /translation="TRFWPRSREKKPKHLLDIISARTIIQETVDHIKELIAPQNILVV
                     TGKKHARELIKQLPEITQKNIIIEPEGKNTAPCIGLAALHIQKKVGDDVMVVLPSDHA
                     IGDSRKYTEVIAAAAQFAAKENALVTIGIKPSSVQTGFGYIEQAASCQLISNREVFHV
                     QSMREKPGFAQAQTFVQSGNFYWNSGMFIWKASIILDEISRYLPDLYSGLMKIKKVLG
                     TPRETAAIRSIYKKLVSISIDYGVMEKADNVFMLRGDFGWSDVGSWDALWEISAKDNK
                     GNAVTGGRNVIFEDTEGSLVYSPQKLVAIVGAKDLIVVETKDALLICKKGRSQDVKKV
                     VETLEAAKTKKYI"
     gene            complement(1047..1184)
                     /locus_tag="VF305_00870"
     CDS             complement(1047..1184)
                     /locus_tag="VF305_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415716.1"
                     /translation="MKKHENKEHDLLIITFDDEKTTLNIIIDELKKGKFTASGDPVYL
                     K"
     gene            1481..2074
                     /locus_tag="VF305_00875"
     CDS             1481..2074
                     /locus_tag="VF305_00875"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX7415717.1"
                     /translation="MTISLSAPLILASASPRRQELLRFVGLKFKTIPAHLNEDYSEGE
                     SPREHVQRLSQDKAIVIAKKYPNAVVLGADTIVVIDKSILGKPENKYQARKMLRKLSG
                     REHKVFTGFTIAHVASKIKQTKVIQSAVKFKTISAAELEWYIACDEPYDKAGGYALQG
                     RGAYFIQSIRGSYTNVIGLPLCEVLETLKKLEAISFG"
     gene            2088..2780
                     /locus_tag="VF305_00880"
     CDS             2088..2780
                     /locus_tag="VF305_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX7415718.1"
                     /translation="MEVNIAANINMIKQRIAAAALKCGRNPDSIKLLAVTKTIPTEYI
                     IKAIDAGITMLGENYVQEAKEKITVLGQRAQWHMIGHLQTNKAKYAVNLFDYIHSVDR
                     LELAQELDKRARLIGRKINILIEVNVSGEKTKNGIPATTAIDLIKVTSKLENLSVCGL
                     MTMAPYSDDPENARPYFCALRNLRDNISRQQISGIQMEELSMGMTDDFEAAIEEGATI
                     VRIGRAIFGERK"
     gene            complement(2809..3960)
                     /locus_tag="VF305_00885"
     CDS             complement(2809..3960)
                     /locus_tag="VF305_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7415719.1"
                     /translation="MTSVPLKPVKQRHSILIVDDEESILSAFKRILADEDYEIHTARN
                     GLEGLNKLRAAQKPFSLIISDQRMPVMNGVQFFTQAKDIFPDAVRILLTGYADTEAII
                     DAINKGGVHLYFTKPWREEELLFHIKQSLSKTELLVENKRLAELIKKQNEELLDLNKT
                     LKEKIEEKTSALLARAEELKASYEKAQMILDGTVKTMSKIVETRDPYTSGHEAQVAII
                     SCKIAKEMGLSENQIEAINVAATLHDIGKISVPSEILTKPGHLSDLEMEIIKTHCQVA
                     YDILKTIKFPYPVADIILQHHERIDGSGYPRGLKGGDILLEARIIGTADVIDAMASHR
                     PYRPALGVGVAMEEIVKCKGTIYDPSVVDACLEIYKTVGKDGFAQIKND"
     gene            complement(4189..>4483)
                     /locus_tag="VF305_00890"
     CDS             complement(4189..>4483)
                     /locus_tag="VF305_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7415720.1"
                     /translation="KRTNSNTLYILDEPTIGLHFADIQKLLDVLKRLVDMGNTIVVIE
                     HNLDVIKCADYIIDLGPEGGPGGGQIIASGTVEKVAGEEKSATGRFLRKILPA"
BASE COUNT         1356 a          946 c          910 g         1271 t
ORIGIN      
        1 acaaggttct ggccccgcag cagagagaaa aagcccaaac atctactgga tataataagc
       61 gcaagaacga tcattcaaga aacagttgac cacattaaag aactgattgc cccgcaaaat
      121 atcttggtgg tgacaggcaa aaagcacgcg cgtgagctaa tcaaacagct tccggaaatt
      181 actcaaaaaa acattattat cgaaccggaa ggtaaaaata ccgcaccctg cataggactc
      241 gcggctctgc atatacaaaa aaaagttggc gatgatgtca tggttgtgct gccttccgac
      301 cacgccattg gtgattcccg taaatataca gaggtcattg cggcggcggc acaatttgcc
      361 gcaaaagaaa acgcgctggt aacaatcggc attaaacctt ccagcgttca aacaggcttt
      421 ggctacatcg aacaggccgc ttcttgtcag ctgatatcaa acagagaagt attccatgtg
      481 caatccatgc gagaaaaacc gggtttcgcg caagcgcaaa catttgttca aagcggcaac
      541 ttttattgga acagcggcat gtttatctgg aaagcctcga taattttaga tgaaattagc
      601 cgatacctgc cggacttata ctcaggcctg atgaaaataa aaaaagttct gggcacacca
      661 agagaaacgg ctgctatccg tagcatttac aaaaagctgg tctcgatttc tattgattac
      721 ggcgttatgg aaaaagcgga caatgttttt atgctgagag gtgattttgg ctggagcgat
      781 gtgggcagtt gggacgcact ttgggaaata tcggctaaag ataacaaagg caacgccgta
      841 accggcggca ggaatgtaat tttcgaagat acggagggtt ctcttgttta tagcccgcaa
      901 aagcttgtcg ctatcgtagg agcaaaggat ttaatcgttg tagaaacaaa agatgcctta
      961 ctgatttgca aaaaaggccg ctcccaggat gtaaaaaaag tagtcgaaac tctggaggcg
     1021 gcaaaaacga agaagtacat ttagttttac tttaaataaa ccgggtcacc gctagccgta
     1081 aacttccctt ttttgagctc atcaataatt atgttaaggg ttgttttttc atcatcaaag
     1141 gtaatgatta atagatcatg ctctttattc tcatgtttct tcacgccgtt aatttttttc
     1201 aggatagaac ctatcctggc attggagttt caggaggaac aaccaggtat cgtcagctgc
     1261 acaattcttt cttcagccaa agcaacacta ataaacactg ataagaaaac gattgttaaa
     1321 aatacactta atattttttt catcatctac ctcgttactt attctggact acattataac
     1381 cccaaaacct gtaaacatgc aaatataatt tataaattga caggcaaaaa tttatttgtg
     1441 atataaacat cattataaaa agacaataga aagtaaaatc atgactatct ctttatcagc
     1501 cccccttatt ttagcttccg cgtcgccaag gcggcaggaa ttgttacgtt ttgtgggatt
     1561 gaagttcaaa acaatccccg ctcatttaaa cgaagattac agcgagggag aaagtccccg
     1621 ggaacatgtc caaagacttt cgcaggataa ggcaattgtt atcgccaaaa aatatcctaa
     1681 tgcagtggtg cttggcgcag acactattgt ggtcatcgat aaatccattt tgggaaaacc
     1741 ggaaaataaa taccaagcaa gaaaaatgct gagaaaactc agcggccgtg agcataaagt
     1801 attcaccggt tttacaattg ctcatgttgc ctctaaaata aagcagacta aagttattca
     1861 atcggcggta aaatttaaaa caatcagcgc cgcagagctg gaatggtata tcgcctgcga
     1921 tgaaccatac gacaaggctg gcggttacgc cctgcagggg agaggcgctt attttatcca
     1981 atcaattcgc ggctcttata caaacgttat cgggctgccg ctttgtgaag ttttggaaac
     2041 attaaaaaaa ttagaagcaa ttagttttgg gtaggtttat aggtttaatg gaagtaaata
     2101 tcgccgccaa cataaacatg attaaacaaa gaatagctgc tgctgcatta aagtgcggcc
     2161 gtaatcctga ttccattaaa ttgttggcgg tgactaaaac cattcccacc gaatatatca
     2221 tcaaggcgat tgacgccggc atcacaatgc ttggcgaaaa ttatgttcaa gaagcaaaag
     2281 aaaaaattac cgtgctaggg cagcgcgccc aatggcatat gattggccat cttcaaacta
     2341 acaaagcaaa atatgccgtc aatctcttcg actatattca ttccgttgac cgcctcgaac
     2401 tagcccaaga attagacaaa agagctcgcc tgatcggccg caagataaat atcttgattg
     2461 aagtcaatgt cagcggcgaa aaaacaaaaa acggcatacc ggcgacaaca gctattgacc
     2521 tgattaaggt cacctccaag ttagaaaatc tttcggtgtg cggattaatg actatggctc
     2581 catattccga tgatcccgaa aatgcccgtc cttacttttg tgctttgaga aatcttcgtg
     2641 ataatatctc tcgtcaacaa atttcgggaa ttcaaatgga agaactttcc atggggatga
     2701 ccgacgattt cgaagcggcc atcgaagaag gggcgacgat tgtccgcatt ggccgggcga
     2761 tctttgggga acgaaaatag tcaacgaaaa accggaattt tttaagggct aatcattctt
     2821 tatctgagca aatccatctt taccgacagt tttgtaaatt tccaagcagg catcaacgac
     2881 tgacgggtca tatattgttc ccttgcattt aactatttct tccatagcaa caccaacacc
     2941 taaagccggc cggtaaggcc gatgggaagc catcgcgtca atgacatccg cagtcccgat
     3001 aattcgcgcc tccagtaata tatcgccgcc ttttaatccc cgcggataac cggacccatc
     3061 tattctttca tggtgctgta aaataatatc ggccaccgga taagggaatt taatggtttt
     3121 caaaatatca taggcaacct ggcaatgtgt cttgataatt tccatttcca ggtcacttaa
     3181 gtgccccggt ttggttaata tttcagaggg tacggatatt ttcccgatat catgaagtgt
     3241 agcagcaacg ttaatggctt caatttgatt ctcagacaat cccatttctt ttgcaatttt
     3301 gcaggaaatt atcgccactt gtgcttcatg accggaggta taaggatcac gggtttccac
     3361 gattttagac atcgtcttta ctgtaccatc tagaatcatt tgtgcttttt cgtaactggc
     3421 ctttagttct tcagcccggg ctaagagagc acttgtcttt tcttctattt tttctttgag
     3481 ggttttgttt aagtccagaa gttcttcgtt ttgctttttg atcagttcgg ccaatctttt
     3541 attttccact aatagttccg ttttggaaag tgattgcttg atatgaaaca gcagttcttc
     3601 ttcacgccat ggttttgtga aataaagatg aacccctccc ttattaatgg cgtcaattat
     3661 agcttcggtg tcggcgtaac cggtcaaaag tatgcggacg gcgtctggaa agatatcttt
     3721 ggcctgagta aaaaattgca caccgttcat caccggcatt ctttgatcgg aaataataag
     3781 ggaaaaaggt ttttgagctg cgcgcagttt attcaggcct tccagcccat tgcgcgctgt
     3841 gtgaatttcg taatcttcat cggccaaaat gcgcttgaaa gcactaagaa tggattcttc
     3901 atcatcgaca attaatattg aatgcctctg ttttacaggt tttaacggta ctgaagtcat
     3961 tctatcatcc ataccctgga aacgttatta agaatgtttt tacattttat tatatatcat
     4021 caacatttta ctttcacgta aaaatattga acatttcgga taatcatgtt aagttattgt
     4081 tattattaat taataattat atgaaattcc tattaccaat ttaaaattct gacctgaacc
     4141 aaaatggctt ttcgtgggaa gggcatccct tattattaat ttgatctctt aagcgggaag
     4201 aatttttctc aaaaatcggc cagtcgctga tttttcttcc ccggccactt tttccactgt
     4261 accggacgca ataatttgac cgccgcccgg cccaccctcc ggcccaagat caataatata
     4321 atcggcacat ttaattacat ccagattatg ttcgattacg acgatggtat tgcccatatc
     4381 cacaaggcgt tttaaaacat ccagcaattt ttgtatatct gcaaaatgca acccaatggt
     4441 cggttcatcc agaatataaa gagtgttgct gttggttctc ttg
//
LOCUS       DASVIM010000046         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993 k141_18400,
            whole genome shotgun sequence.
ACCESSION   DASVIM010000046 DASVIM010000000
VERSION     DASVIM010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18400"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VF305_00895"
     CDS             complement(<1..742)
                     /locus_tag="VF305_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415721.1"
                     /translation="MRFFRKKELIIERKARKYNTSFLQPLAVGIVSAVLVALILIMGL
                     LDIRRSKTNLVGFMEDQALSTISVLQRLTEENFKSIVASPEKAGSNLKNSSQDEATYS
                     KKWVIEAITEMGRKLDEQWKRGKINDNYLRKIAADNNFWYLAVLNPQGNAVYQSGPLQ
                     TDMLDPRELKRGNRNLTTIEVMEKIRAKQGIGFVALRRKNNSGTVVINLDKEGLIYWS
                     LKVAVEKGIKKIGEGHGIVYMQIINDQQK"
     gene            complement(1181..>1507)
                     /locus_tag="VF305_00900"
     CDS             complement(1181..>1507)
                     /locus_tag="VF305_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX7415722.1"
                     /translation="AVYLAVAPKSNSLYAGFGSVKETINKTGYLPVPLHIRNAPTKLM
                     KELDYGKDYKYAHDYHDAYVPQEYLPEKIQGQLFYQPKEAGFEKTIKERIAAWRQKKN
                     NAKDNQ"
BASE COUNT          400 a          321 c          250 g          536 t
ORIGIN      
        1 ctttttgctg atcattgata atttgcatgt aaactattcc atgaccttcg ccgattttct
       61 ttattccctt ttctacggca actttcaggc tccaatagat taatccttct ttatccaggt
      121 ttataaccac tgtgccactg ttattcttac gcctaagggc aacaaagcca ataccctgtt
      181 tggcgcgaat tttttccatt acttcaatag tcgttaaatt acggttgcct ctttttagtt
      241 ctcttggatc cagcatatct gtttgtaaag gaccgctttg atatactgca tttccttgcg
      301 gattcaagac ggccagatac caaaaattat tatccgcagc aattttcctt aggtaattat
      361 cgtttatttt gccccttttc cattgttcgt ctaactttct ccccatctct gtaattgctt
      421 caatcaccca ttttttggaa taagtggctt catcctgact ggaatttttc aaatttgaac
      481 ctgctttttc gggcgaagca actatacttt taaaattttc ttccgttaac cgctgcagaa
      541 cgctgattgt gcttagcgcc tgatcttcca taaaacctac gaggttggtt ttacttcttc
      601 taatatccaa caaccccata atcaagatta aggccactaa aacagcgctg acaataccca
      661 ccgctaaagg ttggagaaaa ctggtattat atttgcgggc tttacgctca ataattaatt
      721 ctttttttct gaaaaaacgc atttttaccc ttatgcctca ttttgccggt tatcttaagc
      781 cattcaaggg aaaagtcaac ggctaatcca agaaataatc gacatttttg tcgaagtagg
      841 ggtaaatctt tctatagata gatatgcttt tgtatttatt atactattta aaaacaaacg
      901 gatatatttc ccgacattta ttgccactaa accaaacggc ttgtttgtag ctaattgcac
      961 tatttttaac gttttatcaa ttaattcaag tacatagaat aataaaatcg tttatggcac
     1021 aattcatgct ttataactgg gtaagttata attttgtcga ctaaaaataa cgggcatgat
     1081 tggccgacag cagaataacc tggaaggtga caagccttct ctcttcactc ctagggaggc
     1141 tccaacaaag cctccctttc cttttgtgta acagcacatg ttattgatta tctttagcat
     1201 tatttttttt ctgccgccag gcggctattc tctcttttat ggttttttcg aaacctgcct
     1261 ctttaggttg atagaatagt tgcccctgaa ttttttccgg caagtattcc tgtggaacat
     1321 aagcatcatg ataatcgtga gcatatttgt aatctttgcc ataatcaagc tctttcatta
     1381 attttgttgg cgcattgcga atatgtagcg ggactggcaa atatccagtt ttgtttatcg
     1441 tttctttgac gctgccgaaa ccggcgtaaa gtgaattact ttttggggct actgccagat
     1501 aaaccgc
//
LOCUS       DASVIM010000047         3033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_957859, whole genome shotgun sequence.
ACCESSION   DASVIM010000047 DASVIM010000000
VERSION     DASVIM010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957859"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VF305_00905"
     CDS             <1..615
                     /locus_tag="VF305_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415723.1"
                     /translation="LLDLSAAFSSLPLPRGKRVGIMTLGGGWGVVASDLCIENNLEVP
                     KLSEDVISRVNKLLPLFWSHANPIDVVAEMNTEIHMTLLEELLQWDGCDAVIHMGIIG
                     RKIMIKAVLESTIAVDKNYDRKFVEDALRLLEDFEQQFVERTVRLMEKYDKPVIGVYL
                     LNDDKTRTITEVEGCKYKGVNFITPERAVKALGKMYKYAQWLRG"
     gene            complement(800..2275)
                     /locus_tag="VF305_00910"
     CDS             complement(800..2275)
                     /locus_tag="VF305_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX7415724.1"
                     /translation="MKSQNRKTFFMFLMAFLVAFFIVSVVEVLRSSFMPSGAPEVQTA
                     AAISSSESSKVPLSFSDLAERVKPAVVNISTTKTYKGRGGFGMPFGRSPFGGPFGDDF
                     FDRFFGDNPQREFKQRSLGSGFIISNDGYIFTNNHVVEKADKILVKVSDEKEYEAKII
                     GTDAKTDIALIKIKPNNSLPVVEIGDSDKVKVGEWVIAIGNPFGLEQTVTAGIVSAKG
                     RVIGAGPYDNFIQTDASINPGNSGGPLFNMEGKVIGINTAIVAQGQGIGFAIPVNMAK
                     SILAELKTKGKVTRGWLGISVQDITEDIAKNLNHKDKSGALVSDVFKGDPADKAGIKT
                     GDIIIEINGKSIKDKHDLLVIIAAMHVGESISIKVLRDGKAMVFQVTVAERKDKPEIA
                     STKESKGYFGIAAQEITAEMSRQLGISRDAGVIVTDVEEGSPADDVGIQPQDIITQIN
                     KVKVTSMKEYTKEMKKAAEKKNVTLLVKRGKSSFFVGLRIE"
     gene            2458..>3033
                     /locus_tag="VF305_00915"
     CDS             2458..>3033
                     /locus_tag="VF305_00915"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7415725.1"
                     /translation="MTFLENIGRSTIKLMKSFVETISFAGKVLYRICKPDTYNSATRT
                     VLINQIYFTSVQILPLFITASVIFGSLLIGIVFQLLKDLGLTQFIGNVLMGLIVTELS
                     PFFTVLLITLRSGSAINAEMAVMKVNREIKTLETFHIDVVNYLIVPRIINGVISLVLL
                     SALFSIVLMSSGILFSRIIFGMSIDVYSNILL"
BASE COUNT          832 a          642 c          618 g          941 t
ORIGIN      
        1 ctgctcgatc tttccgcggc attttcttct ttacctctac ctagggggaa aagagtgggc
       61 attatgactt taggaggggg atggggcgta gtcgcatcag atttatgcat agagaataat
      121 ttggaagtac caaaattatc agaggatgta atttcccgag ttaataaatt gctgccgcta
      181 ttttggagcc atgccaatcc aatagatgtc gtcgcggaaa tgaataccga aatacacatg
      241 acacttctcg aagagcttct tcaatgggat gggtgcgacg cggtaatcca catgggtatt
      301 atcggaagaa aaataatgat caaagccgtg ctggaatcaa ctattgctgt agacaagaat
      361 tatgatcgta aatttgtaga ggatgcttta cggcttctgg aagattttga gcagcagttt
      421 gtcgaacgca cggtaagact aatggaaaaa tatgataaac cggtaatagg ggtttatttg
      481 cttaatgatg ataaaacgcg gacgatcacc gaagttgaag gctgcaaata taaaggagta
      541 aactttatta ctccggagcg agccgttaag gcattgggta aaatgtataa atatgcccag
      601 tggctgaggg ggtaataaat aattgctccg tcaagattaa atttcaaaac acacattagc
      661 catactgata caaccgccgt tcaagttaaa ggcaatgttt cacgtgaaac attttttatt
      721 cccaggccaa aattacaaaa aataaaaaag gcaggccata atagcctgcc tttttggtat
      781 ttccccataa ttcatctttt tattctatgc gcaatccaac aaaaaaactc gatttaccac
      841 gcttaactaa gagagtcaca tttttctttt cggcagcctt tttcatttcc ttggtatatt
      901 ctttcattga tgtaactttt actttgttta tttgagtgat tatgtcttga ggttggatgc
      961 caacatcatc tgccggactg ccctcttcca catcagtaac tattacaccg gcatcgcggg
     1021 aaattcctaa ttggcgtgac atttctgcag ttatttcctg ggcggcgatg ccaaaatacc
     1081 ccttggactc ctttgtagat gctatttcgg gtttatcctt gcgttccgcc acggtaactt
     1141 ggaacaccat tgctttacca tcgcgcaata ctttaatgga gatcgattca ccaacatgca
     1201 ttgcggcaat tatcacaagt aaatcatgtt tgtctttaat ggacttgcca ttgatttcta
     1261 taattatgtc acctgtttta attcctgctt tgtccgctgg atcaccttta aaaacatcgg
     1321 ataccaaggc gccgctcttg tctttgtgat tcaggttttt cgcgatatct tccgtaatat
     1381 cctgtacgga aattcccagc caacctcgag ttactttacc tttcgttttc aattccgcca
     1441 gaatgctttt tgccatgttc accggtatag caaagcctat accctgacct tgagctacga
     1501 tggccgtgtt aataccgatt acttttcctt ccatattaaa taatggccca ccgctgttgc
     1561 ccggattaat ggatgcgtcg gtttggataa aattgtcata aggtcctgca ccgataacgc
     1621 gccccttagc gctaacgata ccggcggtga cggtttgttc caaaccgaaa ggattgccga
     1681 tggcaataac ccactcacct acttttactt tgtccgagtc tcctatttcc acaaccggca
     1741 agctgttgtt gggtttgatt ttgattaagg cgatatctgt tttagcatcg gtgccgataa
     1801 tttttgcctc atattccttt tcgtcggaga cctttaccag aatcttgtcg gccttttcta
     1861 ccacatggtt attggtgaaa atataaccgt cattgctgat gatgaatccc gaaccaaggc
     1921 tcctctgctt aaattcacgt tgcggattgt cgccaaaaaa gcgatcaaag aaatcgtcgc
     1981 cgaaaggccc gccaaaaggc gaccgcccaa aaggcatacc aaaaccgccc cggcctttgt
     2041 atgtctttgt cgtgctgatg ttcactaccg cgggcttaac acgctcggcc agatcggaaa
     2101 aagaaagagg aacttttgat gattccgaag aagaaattgc cgccgccgtt tgaacttccg
     2161 gcgctcctga aggcataaag gaagaacgta gaacttccac cacggaaaca ataaaaaagg
     2221 cgaccaaaaa cgccattaag aacatgaaaa aagtttttcg attttgactt ttcatataat
     2281 cctccgcttt gttggtaaaa aattgcggct atgaatgccg cgctacagac tttgctttct
     2341 tgatattgcc ggtcaatata acgattgtct tatatttgtc aatgaaatat tggttttggc
     2401 ggtttgaagt ctggacaatc atgtgctagg attaattatc ttactggtga aacgcttatg
     2461 actttcttgg aaaacatagg acgctcgacg ataaaattaa tgaagtcttt cgtggaaact
     2521 atttcctttg cgggaaaagt tttataccgc atatgcaagc ccgatactta caatagtgcc
     2581 acgcgcacgg tattgattaa tcaaatatac tttacctctg tacaaatttt accactattt
     2641 atcacggcat ccgtgatatt cgggtcactg ctcatcggca tagtttttca actcttaaaa
     2701 gatttagggc ttactcaatt tattggcaat gttcttatgg gtttgatcgt tactgaactt
     2761 tctccttttt tcacggttct tttgattaca ttacgttccg gttccgccat taacgcggaa
     2821 atggctgtaa tgaaggtcaa cagagaaata aaaacattgg aaacattcca tatcgatgta
     2881 gtcaactatt tgattgtacc gcggataatt aatggcgtta tatctttggt cttgttgagc
     2941 gctctttttt ccattgtctt gatgagcagc ggcattttgt tttcacggat aattttcggc
     3001 atgagtatcg atgtatattc aaatattctg tta
//
LOCUS       DASVIM010000048         8336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1214061, whole genome shotgun sequence.
ACCESSION   DASVIM010000048 DASVIM010000000
VERSION     DASVIM010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214061"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VF305_00920"
     CDS             complement(<1..197)
                     /locus_tag="VF305_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX7415726.1"
                     /translation="MGKILSVNISKKKGVKKHNVTSSRALIDLGLEHDAHIGMELRQI
                     SLLAAESIEKIRNKGLNVSYG"
     gene            complement(319..696)
                     /locus_tag="VF305_00925"
     CDS             complement(319..696)
                     /locus_tag="VF305_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEX7415727.1"
                     /translation="MGKKIITSDKLPVLGPYSAAVETGGFIFISGQIPLNPVTGKVIT
                     NIKSATRQILVNMQTLLQENGLKLDNIVKTTVFLKNMADFPTVNEIYADFFLEGPPAR
                     STIEVSALPKGAPIEIEAIAIRK"
     gene            complement(734..1315)
                     /gene="metW"
                     /locus_tag="VF305_00930"
     CDS             complement(734..1315)
                     /gene="metW"
                     /locus_tag="VF305_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX7415728.1"
                     /translation="MKLDHQIIAKIINEEAHVLDLGCGSGELLAFLTQEKKALVQGIE
                     LDEDQMHQCVERGLTVLQGDIESGLVDYPDKSFDFVILNQIMQEIKKADFVIQESLRV
                     GKRVIVGFPNFAHIKSRFTLVFRGKTPINEALPYYWFETPNIRFLTISDFIDYCGKKE
                     IKIEQEYFLGKSGQIHILPNLFAQNALFVITRR"
     gene            complement(1361..2482)
                     /locus_tag="VF305_00935"
     CDS             complement(1361..2482)
                     /locus_tag="VF305_00935"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX7415729.1"
                     /translation="MKKDSIGIVTPQFFSFAEPPDNLQLESGRSLGPITLAYETYGKL
                     NTKKSNAILVCHALSGDAHAAGIHKEDGTPGWWDNMIGPGKALDTNLYFVICSNVIGG
                     CKGSTGPSSINPETRKPYALDFPMITLADMVEAERHLIDSFGIEKLLCVIGGSMGGMQ
                     ALQWASAYPERIIAAIPIATALKHSPQQIAFNEVMRQAIMADPVWREGYYYEHGQPEN
                     GLAVARMIGHITYMSDLSMEEKFSRKLKNENLSFQFKDDFEVEGYLRYRGYNFVKRFD
                     ANSYLYITKAIDYFDLSGEKFMPAVKKTAVRFLVISFKSDWLYPTHQSQDIVRLLKRK
                     HAYTTYFDLISTYGHDAFLIEIDDETRLIKNFLANISSD"
     gene            complement(2548..3492)
                     /gene="nadA"
                     /locus_tag="VF305_00940"
     CDS             complement(2548..3492)
                     /gene="nadA"
                     /locus_tag="VF305_00940"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX7415730.1"
                     /translation="MVLLQEITAKEEIMEIADKIKKLKQEKNAVILAHNYQLPEIQDI
                     ADFVGDSLGLSMAAARTNADIIVFCGVHFMAETAKILSPKKKVLLPDKNAGCPMADMI
                     DAEGLKALQASHPDAVTLCYVNTSAAVKAQCDYCCTSSNALKMVQHILLTHEEIIFIP
                     DKYLAQYVSAQTKQDFITWEGYCPTHARILPENIIQAKKLHPQAKVIVHPECKPDLTT
                     LADLVASTEKMCSYVKNTPETEFIIGTEIGIIHRLKKENPGKNFYPATDKAVCPNMKR
                     INLEKVLWSLEDLSYEITVPPDIMDKARLSIERMLQTT"
     gene            complement(3762..4997)
                     /locus_tag="VF305_00945"
     CDS             complement(3762..4997)
                     /locus_tag="VF305_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415731.1"
                     /translation="MTDNLREMIDSIQLIDQHAHPGFAGYFEDFPPEKRILIAIDPYR
                     NPEDSSAGFPYLREAHYEAYQKIYGFSREAINDPTQKDDLVREYDRQRNNLKHLIDKT
                     MDAAGVEILIANAFLHSDLKNKPRIKFIPTVDPLLFPFDNTYLTSRSPLTTSYLAAFE
                     HILNIIKVKNKYSSQSFADYLEFVDRVLAQFVNNGAVGFKLFIAYVRNTYCEKVEEIE
                     GSLLFERAKRGESAAYRRLQDLLVWYILRKSLEYNLPIQWHFALSDPYIGCFDCLNLS
                     EMICDPIASKAKQVVLHGGYPRFDHAELLALAGGFIPNNIYLDISGRMMFMSHPRILA
                     GTLRKWLEKPILWDKIMYGSDAFCGERYIYVASKVGRDAIYFALKSMIDDEIINENTA
                     IIIARKILRENARKLYRLQ"
     gene            complement(5090..5560)
                     /locus_tag="VF305_00950"
     CDS             complement(5090..5560)
                     /locus_tag="VF305_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415732.1"
                     /translation="MWEKNAPIEKTYKKIIIQKFEVDQDMEKDYPGATVTCESTAMNE
                     LLKKSDVSKIEKVRSNLSREAGALIVKIRVTTLRTIGSTGRGLGGAFVGSPEMAVNLK
                     LIDAATGQTLREKNLSTANNPPAVSSISGSADRTLPLDMGKIIAQYIAEVVRGN"
     gene            complement(5903..7072)
                     /locus_tag="VF305_00955"
     CDS             complement(5903..7072)
                     /locus_tag="VF305_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX7415733.1"
                     /translation="MNYSYRLLSFILILTILMAGTIDGKVQAESSLSAGQALIEAIKI
                     TDPLTFCGEPVPLNEADVKERLERELLLSLDNSDDVILWLKRANRYFPHIEKVLKNNS
                     LPDDLKYIAIVESALKPLVASSKGAVGYWQFIESTGTKYGMEINNDIDERRNLFISTE
                     AAAKYLKDLYALFGSWTLAAAAYNMGEEGLKAEILVQKVNNYYQLYLYQETQRYVFKI
                     LAVKIIMSNPEKYGYVIARDDLYQPVQFDSIEISASQPVPLYIIAQAARTYFKIIKDL
                     NPHIKNSYLPAGKYHLLLPQGSAMGFSARYENLLAQWLTEGKDCIYIVKKGDNLSIVA
                     DRFNVPVKAIIIWNGITNGKRVLPGDKLFIFSDTLKSENSTNQNTTAEKPVLKLP"
     gene            7512..>8336
                     /locus_tag="VF305_00960"
     CDS             7512..>8336
                     /locus_tag="VF305_00960"
                     /inference="COORDINATES: protein motif:HMM:NF025499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HEX7415734.1"
                     /translation="MSMQEKSLFSKNDLQQMKNLGLSSDDVGKQLAAYRYGSNFSKLN
                     RPCIIKDGIFSFTPTQRKKLIALYDKESVKYKLIKFVPASGAASRMFAQWFPASKNGS
                     FGSAALDQSFLRDLKRFPFFSLIEKDKRGRTLLGKKNIKGLLNFILSSNCLNFGQMPK
                     ALIPSHRYLTGEMRTALEEHLCEAKQYICNKNAVCRLHFTLLRDQKTDVARFLKTMIV
                     KYEKLYKVKYKVTLSIQGSSTNTVAVDENKLPLRDANGVIVFRPGGHGTLLKNLNSL"
BASE COUNT         2461 a         1648 c         1650 g         2577 t
ORIGIN      
        1 tcgccataac ttacatttaa tcctttattc ctgatttttt caatactttc agcagccagc
       61 aaacttattt gccgcaattc catcccgata tgggcatcgt gttccagacc caagtcaatc
      121 aaggcgcggc ttgaggtaac attatgtttc ttaacgcctt tctttttgct gatattgact
      181 gataatattt tacccatagt tgttttaccc cgttagagaa agcccccaac tcctgttggg
      241 gtggtaaaat cctgtaagac agatggtggt ttaataccgc catctgaaat cgcaaagcac
      301 ccgttgctct aacggggttt actttcttat cgcaattgct tcgatttcaa tcggagcacc
      361 tttgggcaga gccgaaactt caatagtcga gcgagctgga ggcccctcga gaaaaaaatc
      421 ggcgtaaatt tcattgacgg taggaaagtc tgccatattt tttaaaaaga cggttgtctt
      481 tacaatgtta tccagcttga ggccgttttc ctgaagcaag gtttgcatgt ttacaagtat
      541 ttgccgtgtg gctgacttaa tattcgtgat aaccttgccc gttaccggat taagcggtat
      601 ttgtccggat ataaaaataa agccaccagt ttcgacagcc gcagagtaag gtcctaaaac
      661 aggaagttta tccgaagtta taattttctt tcccatacaa gctccatttc gaaatgctac
      721 acgtcatcag ttgtcaacga cgagtaataa cgaagagggc attctgagca aataaattgg
      781 gcagaatatg aatctgtccg gatttcccca aaaaatattc ctgctcaatt ttaatttctt
      841 ttttgccgca gtaatcaata aaatcactga tagtcaaaaa gcgaatgttc ggcgtctcaa
      901 accagtaata gggcaaagcc tcatttatcg gcgtcttccc gcgaaatacg agagtgaaac
      961 gggacttgat gtgggcaaaa ttggggaatc cgactattac gcgttttcca actcttagag
     1021 attcctgaat gacaaaatcg gcttttttta tttcttgcat gatttgatta agaatcacga
     1081 agtcaaaaga tttatcggga taatcaacta agccgctttc gatatctccc tggagcacag
     1141 tcaggcctcg ttcgacgcat tggtgcattt gatcttcgtc caactcgata ccttgaacga
     1201 gagctttttt ttcctgcgtg aggaaagcaa gcagttcacc gctgccgcag cctaaatcta
     1261 aaacatgcgc ttcttcattg ataattttgg caataatttg atgatcgagt ttcatttata
     1321 aaacaacttt cttcgctttt ttcggcaaat tataacaata tcaatcagaa ctgatattgg
     1381 ctaaaaaatt ttttattaac cgtgtctcgt cgtcgatttc aattaaaaaa gcatcatgcc
     1441 cgtaggtcga gattaaatcg aaataggtag tataagcatg ttttcttttc aagagacgga
     1501 caatatcttg cgactgatga gttggataaa gccaatccga tttaaaagaa atcaccagaa
     1561 acctgaccgc cgttttctta acagcaggca tgaacttttc gccggatagg tcaaagtagt
     1621 ctatagcttt ggttatgtaa agataagaat ttgcgtcgaa tcgtttgaca aagttatatc
     1681 cgcggtaacg cagatagcct tctacttcaa aatcatcttt aaattgaaag ctcaagtttt
     1741 cgtttttaag tttacgcgaa aatttttcct ccatagaaag atcgctcata taagtaatgt
     1801 gcccgatcat gcgggctaca gccagtccgt tttccggctg tccgtgttca taataatatc
     1861 cttcacgcca taccggatcg gccattattg cttgtcgcat gacttcatta aaagcaattt
     1921 gttgcggtga gtgttttaac gcagtggcaa tgggtattgc cgcgataatc ctttccgggt
     1981 aggctgatgc ccattgcaac gcttgcattc cacccatgga gccgccaata acgcaaagga
     2041 gcttttcaat accgaaagaa tcaattaaat gccgctcggc ctccaccata tccgcaagcg
     2101 tgatcatagg aaaatcaaga gcgtaaggtt ttctggtttc tggatttatt gaagaagggc
     2161 cggtgcttcc tttacagcca ccgattacat tggaacagat aacgaagtaa agatttgtgt
     2221 ccagagcttt acccggtcca atcatattat cccaccagcc gggagtaccg tcttctttat
     2281 ggatgccagc agcatgggca tctcccgaaa gagcatggca aaccaaaata gcatttgatt
     2341 tcttggtgtt caatttgccg taggtttcgt aagccaaagt aatcggacct aaactccggc
     2401 cagactctaa ttgtaaatta tcaggcggct cggcaaaaga aaaaaattga ggagtaacaa
     2461 ttcctattga atcttttttc ataaagttat tttccggata taatctaatt cgctattttt
     2521 cgctaagcta tttgacgcct gttaaattta tgttgtttgc agcattcttt ctatggatag
     2581 ccgtgctttg tccataatat ccggaggcac tgtaatttcg taagataaat cttcgagcga
     2641 ccagagaact ttctccaagt ttatccgttt catattgggg cagacagcct tatccgttgc
     2701 cggatagaaa ttctttcccg gattctcttt ctttaatctg tggattatac ctatttccgt
     2761 accgataatg aactcagttt cgggggtatt ctttacataa ctgcacattt tttctgtaga
     2821 tgcaacaaga tcggcaagcg tagttaaatc cggtttacat tccgggtgca caataacctt
     2881 agcttgtgga tgtaactttt tagcctgaat aatattttcc ggtaagattc tggcatgagt
     2941 ggggcaatat ccttcccaag taataaaatc ctgtttggtt tgtgcggaaa catattgcgc
     3001 cagatattta tcgggaataa aaatgatctc ttcatgggtg agtaaaatat gctgcaccat
     3061 ttttaaggcg ttggatgaag tgcagcaata atcgcactgg gctttaactg cggccgaagt
     3121 gtttacatag caaagagtta cagcatcagg atgacttgcc tgaagagcct ttaaaccctc
     3181 ggcatcaatc atatcagcca tcggacaacc ggcattttta tccggcaata aaactttctt
     3241 cttaggagat aatatctttg ctgtttccgc cataaaatga acgccgcaaa aaactataat
     3301 atcagcattt gtgcgggctg ccgccatact taagcccaaa gaatcgccga caaaatcggc
     3361 aatatcctga atttcaggaa gctgataatt gtgagccaga atgacggcat ttttttcttg
     3421 ctttagtttt tttattttat cagcaatttc cattatttct tccttggcgg tgatctcttg
     3481 taataaaacc aagtttcttc gatgattgta tattaaaaca gtaattaatc acagaccata
     3541 tcttgacctg gtcaaaagct tcatataaat aacttgatag gcgatatact atttctttgg
     3601 tagtaattaa gtcagaaaaa aacatttgtc aagaaatatg gtaaaagatt ggctaataat
     3661 aacaagttgg cattgtgtga tttaatcggt tcttcacgta atgtttcttt ataagggcgg
     3721 atgagcctgg aagtggcaaa cgctacggga agatttaata ttcattgaag tctatagagt
     3781 ttccgggcat tttcccgcag aatcttgcgt gcaataatta tggcagtatt ttcattaatt
     3841 atttcatcat caatcatgct tttcagagca aagtaaatag catcgcggcc gactttggaa
     3901 gcaacatata tatacctttc gccacaaaaa gcgtcagaac catacatgat cttatcccaa
     3961 agaattggtt tttccagcca ctttcttaaa gtaccggcaa gaatgcgcgg gtggctcatg
     4021 aacatcatgc gtccggaaat atcgagataa atattattgg gtatgaatcc accggctaaa
     4081 gctaataatt ccgcgtggtc aaagcgcgga taaccaccat ggagcacaac ctgttttgcc
     4141 ttgctggcaa taggatcgca aatcatctcg gaaagattaa gacaatcaaa acaaccgata
     4201 taggggtcgg ataaggcaaa atgccactgt ataggcaaat tatactctaa gcttttccgc
     4261 aggatatacc aaacaagcaa atcttgcagt cttctataag cggcagattc tcctcttttg
     4321 gcgcgttcga aaagcaatga accttcaatt tcttccactt tttcgcaata ggtattacgc
     4381 acataagcaa taaataattt gaagccgact gcaccgttat tcacgaactg agcgaggacc
     4441 ctatccacaa attcgagata atctgcaaaa ctttgcgaag agtatttgtt tttgaccttt
     4501 ataatattta aaatatgttc aaaagcggct aaatatgagg tggttagagg acttctagag
     4561 gtcaagtatg tattatcaaa gggaaataag agaggatcaa ctgtggggat gaatttaatt
     4621 cgtggtttat tctttaagtc gctatggagg aaggcgttgg caataagaat ttccacgccg
     4681 gcggcatcca ttgttttatc aatcagatgc tttaaattat tacgttgacg gtcgtattct
     4741 cttactaagt catctttttg ggtgggatca ttaatggctt cacgactgaa accgtaaatt
     4801 ttctgatatg cttcataatg agcttcacgc aaatagggga aaccggcgct ggagtcttcg
     4861 gggttgcgat aaggatcgat agcgataaga atcctctttt ccggtggaaa atcctcgaag
     4921 tagccggcaa atccagggtg agcatgctga tcgatcaatt gaatgctatc gatcatttct
     4981 ctcaggttgt ccgtcatttt cttcctcctc caataaccgc tgcttttagc tcgacaatta
     5041 gatccaccaa cagatggaaa agtgtataag tgttaaaatt attgcagcgc taattacctc
     5101 tgacaacttc ggcaatgtat tgggcgatga tcttacccat gtccaaaggc aatgttcgat
     5161 cggcgctgcc actgattgag gaaacagcag gaggattatt cgcagtggaa aggttttttt
     5221 cgcggagagt ttgtcccgtt gccgcatcga tcaatttcaa attaactgcc atttccgggc
     5281 tgcctacaaa tgctccgccc aaaccgcggc cggtgctgcc gatagttctt agagtagtaa
     5341 cacgtatttt tactataaga gcgcctgctt cccgtgacaa gttggaacga actttttcaa
     5401 tttttgagac gtcacttttt tttagaagtt cgttcatagc tgtgctttcg caagtgacag
     5461 ttgcaccagg gtaatctttt tccatgtcct gatcgacttc aaacttttga atgattattt
     5521 ttttataggt tttttctata ggcgcatttt tttcccacat ggattctttc tggaatttga
     5581 ctgattttga tccaccgcag gagaatataa aaattatgca aacaaagatt gatatagaga
     5641 aaaaaacttt ttttgatttt ttggtcatta tttttatctg aaaaacgtaa ttaatttata
     5701 ttaaaatatt ggtaattaac tataataatt tttgtataaa aacaaccgcg aaaagtctgg
     5761 tgcaaaaatg ttaatattat aggacagtta gtggtaggat tcgttatgag cagaatagaa
     5821 ataagcttat ccaaccggcc tttctttcaa aatatcggtg ctttccaatt ctttaaacat
     5881 tatactaaag tgtcttttct ttctagggaa gcttcaaaac cggtttttct gctgttgtat
     5941 tttgattagt gctattctcc gattttaatg tatcggaaaa gataaaaagc ttgtctccgg
     6001 gtaatactct tttaccattg gtgataccgt tccagattat tattgctttg accggcacat
     6061 taaaacggtc ggcaacaatg gacaaattat cacctttttt aactatgtaa atacagtctt
     6121 ttccctcagt aagccactgc gccaaaagat tttcgtaacg tgcggaaaaa cccattgctg
     6181 atccctgagg aagtaataaa tgatatttgc cggcgggcag ataagagttt ttgatatgag
     6241 gattaaggtc tttaattatt ttaaaataag ttcgtgctgc ttgtgcaata atatacaatg
     6301 gtaccggttg gctggcagat atttctatac tgtcgaattg cactggctga tagagatcat
     6361 ctctggcaat aacgtagcca tatttttccg gattagacat aattatcttt actgctaaaa
     6421 ttttaaaaac atatctttgc gtttcctggt ataagtaaag ctgatagtag ttatttactt
     6481 tttgcacgag tatttccgct tttaagcctt cctctcccat gttataggcg gctgcagcca
     6541 gtgtccagga accaaaaagc gcataaaggt ctttgagata ttttgccgct gcttccgtgg
     6601 aaataaaaag attgcggcgt tcgtcaatat cgttattaat ttccataccg tatttagtgc
     6661 ccgtgctttc gataaattgc caatatccca ctgctccttt acttgatgct acgagcggct
     6721 ttaaagcact ttcaacaatg gcgatgtact ttagatcgtc aggcagggaa ttatttttta
     6781 aaactttttc aatatgagga aaatatcgat tggctcgttt gagccagaga ataacatcgt
     6841 cgctgttatc caaagataga agcaattctc tttccagcct ttctttgaca tccgcttcat
     6901 ttaagggcac cggttcgccg cagaaggtga gaggatcggt gatcttaatt gcttcgataa
     6961 gagcttgtcc cgccgaaagg ctcgattctg cctgaacttt gccgtcaatt gtgccggcca
     7021 tgagtattgt taaaattaaa ataaaactta ataatcgata tgaataattc attacaatta
     7081 ctcccataat tacacatatt agtttacagg ggaatggcgg ggttgtcaaa tatttccaac
     7141 agcattgtcc gaaggcaatt ttattcgtga gatacaaatg tcaaaaacga agtgcataac
     7201 ctaataacct aatcacctga taacctgaag gtcacacgag atgacgcgag ggcacgcgag
     7261 gtcacacgtt gaaatttgca agtcgaatcc gcctccggtg gactcaaaat atattaatta
     7321 tagaaaaaag aataattata tcaaaaaata aaatttactt agcagttttt aattacttgt
     7381 taacgctaaa aaatgttttt cgcgtcaaga cacagtcagc tattttattt ggggggaaga
     7441 atcgaataaa tatcatttct tcttgccaat gtttaacgtt ggtgttatgc gaaagaacaa
     7501 ttataaatat tatgagtatg caagaaaaat cattattttc gaaaaatgat cttcagcaaa
     7561 tgaagaatct gggcttaagc tcagacgatg tgggaaagca acttgctgcc taccggtatg
     7621 gttccaattt ttcaaaatta aaccggcctt gcataattaa agatggcatt ttttccttta
     7681 cgccgacgca aaggaaaaaa ctaattgccc tttatgacaa agaatctgta aaatacaaac
     7741 tgataaaatt tgtgcccgct tccggtgctg ccagccgaat gtttgcccaa tggtttcctg
     7801 caagcaaaaa tggcagtttt gggtctgcag ctctcgatca atcatttctc cgggatttaa
     7861 aaagattccc ctttttcagc ttgattgaaa aagacaaacg cggcagaacg cttcttggta
     7921 aaaaaaacat caaaggtttg ttaaatttta ttctgtcttc caattgctta aatttcgggc
     7981 aaatgcctaa agctttaatt ccttctcatc gttatcttac gggcgaaatg cgcacagctc
     8041 tcgaagagca tctttgcgag gcaaagcaat atatttgtaa taagaatgct gtctgtcgtc
     8101 tgcattttac tttgcttcga gatcaaaaaa cagatgtagc caggttttta aaaacaatga
     8161 tcgtgaaata tgaaaaatta tataaagtaa agtacaaggt aactttatcg atacagggat
     8221 catcgactaa tacagtggcg gttgatgaaa ataaattgcc tctaagagat gcaaacggcg
     8281 taatagtttt ccgtcccgga ggccacggaa cactgctgaa aaacttaaac agcctg
//
LOCUS       DASVIM010000049         5320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1044221, whole genome shotgun sequence.
ACCESSION   DASVIM010000049 DASVIM010000000
VERSION     DASVIM010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044221"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VF305_00965"
     CDS             complement(<1..339)
                     /locus_tag="VF305_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX7415735.1"
                     /translation="MLESMGFVFFLVFIALVFDFLNGFHDSANSISTVVSTRVLSPKF
                     AVLWAAFFNFVAAFLIGTEVANTVGRGIIHLDIVDNLLILSALGGAIIWNIVTWYYGL
                     PSSSSHALIGG"
     gene            complement(332..952)
                     /locus_tag="VF305_00970"
     CDS             complement(332..952)
                     /locus_tag="VF305_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX7415736.1"
                     /translation="MRLFPKTENFFEYFEELADKIEEGGQLFLEMAQTRDFSDVRVAK
                     LKEIEHEADGITHKTYEKMHKTFLTPIDREDIYALVNKMDSIMDVIEATAIRIHLYKV
                     KKPADEIIKQAQILNDAIKKIKSIVHAMRNMKNSAMILDCCVEINTLENAGDVVLRTI
                     MANLFENEKDAIELIKWKDIFQLLEEAIDVCEDVSNIVEGIVLKHA"
     gene            complement(1060..1548)
                     /locus_tag="VF305_00975"
     CDS             complement(1060..1548)
                     /locus_tag="VF305_00975"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7415737.1"
                     /translation="MTTLNIKFRRQIKPTDIEAIERIVRSSGVFTSAEMEIALELPEE
                     KLDKGPASSYEFLFAEDEKHILGYTCFGLIPMTSASYDIYWIAVDEHLRGSGLGKKLM
                     AKSEEIIKSLSGRRIYVETSSRNPYRPTQNFYERCGYHREAVLNNFYAESDSKIIYLK
                     EL"
     gene            complement(1579..2562)
                     /locus_tag="VF305_00980"
     CDS             complement(1579..2562)
                     /locus_tag="VF305_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7415738.1"
                     /translation="MKKIVILHSDVAPDAGEDELDCLRQADTIAEALLQLGYDPVLIP
                     FVFDLTRTITALNSLCPEVVFNLVETVAGKGSLIYFAPALLDYLKIPYTGCSTEAIFL
                     TSNKPLAKKIMHEAGIATPEWVTEAGIRSGSSSVDTYIMKAAWEHASVGLDESCVVKL
                     TGSTDLLALINERKKNLVGSLFAEAYIDGREFNVALIAGKSGVEVLPTAEMLFQGYAP
                     DKLKLLDYRAKWVEDSFEYNNTVRTMDFSDEDKEIIADLQEIAKRCWKLFDLRGYARV
                     DFRVDKSGNPWVLEINANPCLSLEAGFVAAVEKAALKYHEAIELIINDALK"
     gene            complement(2595..3599)
                     /locus_tag="VF305_00985"
     CDS             complement(2595..3599)
                     /locus_tag="VF305_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415739.1"
                     /translation="MSRTGKKLKIGITYDLRDEYLKEGYNLEDTAEFDLLDTIESIEK
                     VIIDNGFQADRIGNVKALTRQLVAGNRWDLVFNVAEGLHGFGREAQVPALLDAYNIPY
                     TFSDPLGHALTLHKGMTKHVLRDMGIPTPDFAVVSYESEIADVKLPLPLFAKPVAEGT
                     GKGITSLSKITNREELYRICKYILQTFKQPALVETYLPGREFTVGILGSGKNAKVLGA
                     IEVILKPAAEKNAYSYENKEHYDRLVQYILVDDDEAKQAKEISLCLWRGLDLKDAGRV
                     DLRSDACGAPHFMEVNSLAGLNPKRSDLPILCNLLGMSYDELINSIIKSALRRTNINN
                     "
     gene            complement(3638..4948)
                     /locus_tag="VF305_00990"
     CDS             complement(3638..4948)
                     /locus_tag="VF305_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEX7415740.1"
                     /translation="MENETLFNEEEEPPGLLTGLVKVENEVNEFFPLNRDTHRPYPAK
                     QKPERLLFPFKDSPRSRIFRNRFYPDITRFEWFDWHWQLRNAIRNVDTLSQMLNLSDN
                     ERQAMMHHSGALPVSITPYYASLLNPDDPSAPLRRCVVPVVDECLHTAGEEEDPLCED
                     ADSPVPGIIHRYPDRVLFLVTDTCSTYCRYCTRSRIIERSNQNFVDFEKWEKAIEYIE
                     NNQNVRDVLLSGGDPLTLSDEKLDWLLMRLHKIPHVELLRIGTKVPVVLPQRITPVLT
                     AMLKKYHPLWLSIHITHPDELTPEMSAACIRLADAGIPLGSQTVLLSGINDDVATMIR
                     LVHGQLQIRVRPYYLYQCDPIPGSSHFRTPISKGLEIIQGMRGYTTGYAVPTYVVDAP
                     GGGGKIPLLPEYAIGWEKGALLLRNYEGNLFRYPDNHAESRDIH"
BASE COUNT         1561 a         1154 c         1065 g         1540 t
ORIGIN      
        1 accgccaatc aaggcatgag aggaactgct ggggagtcca taataccaag taacaatatt
       61 ccaaatgatg gccccaccaa gagcagacaa aattaacagg ttatccacga tgtctaaatg
      121 gattatacct cgaccgacag tattggcaac ttctgttcca atgagaaaag ccgcaacaaa
      181 gttaaagaat gccgcccaga gtacagcaaa ttttggtgaa agaacgcggg tggaaacaac
      241 agtggatatg gaattggcgg aatcatgaaa accgttgaga aaatcaaaaa ccagtgcaat
      301 gaaaacaaga aaaaacacaa aacccattga ctcaagcatg tttcaggacg atcccttcta
      361 caatgttgga cacatcttca catacatcaa tggcttcttc tagaagttga aaaatatctt
      421 tccattttat aagctcaatg gcgtctttct cgttttcaaa aagattggcc ataatagttc
      481 ttaaaacaac atcgccggca ttttccaggg tattaatttc tacgcaacag tcaagaatca
      541 ttgcagagtt tttcatgttg cgcatcgcat ggacaatgct tttaattttt tttatcgcgt
      601 cattcaggat ttgcgcctgt ttaatgattt catcagccgg ctttttaacc ttataaagat
      661 gaatgcggat agcggtggct tcaatgacgt ccataatact atccatttta ttaacaagtg
      721 catagatgtc ttcacggtct ataggtgtaa ggaatgtctt atgcattttt tcatacgttt
      781 tatgggtaat gccatccgct tcgtgctcaa tttccttaag tttggctact ctcacgtcag
      841 aaaaatcacg agtttgagcc atttccagaa aaagttggcc tccttcttca attttgtcgg
      901 ctagctcttc aaaatattca aaaaaatttt ctgttttagg aaacaggcgc atgagaacct
      961 cccttcttct ttccttaaaa ttaatatttt aaagtcaggc agtcctctag cgaattattg
     1021 gagaaaatgc aagctgttta acttgctgag taaatggatt tacagctctt tgagataaat
     1081 aattttgctg tcgctttcgg cataaaaatt atttaaaacc gcttcacgat gatagccgca
     1141 tctttcataa aaattttgag ttggacggta tggattacgg gaagatgttt ccacataaat
     1201 acgtctgcca cttaaacttt tgataatttc ttcggatttt gccattaact ttttgcccaa
     1261 gccgctgccg cgtaagtgct catccacggc aatccaataa atatcataac tagcggacgt
     1321 catcggtatt agaccgaaac aagtatatcc caaaatatgt ttttcatctt cggcaaacaa
     1381 aaactcataa gagctggcgg gtcctttgtc cagtttttcc tcgggtaatt ctagggcaat
     1441 ctccatctcc gcgctagtaa aaacgccact tgaccttacg atcctttcaa ttgcctcaat
     1501 gtccgttggc ttaatctgcc gcctgaattt tatattaaga gtagtcataa gaaatgtttg
     1561 agtatttctc ttccattttt atttcagagc atcgttgata ataagctcaa tagcttcatg
     1621 atattttaaa gctgcctttt cgactgcggc gacaaaacca gcctcgagtg acagacacgg
     1681 attagcgtta atttccaata cccagggatt gccgcttttg tcaacacgaa aatcaacccg
     1741 tgcatatccc cgcagatcga aaagcttcca gcaacgttta gctatttcct gtaaatctgc
     1801 aataatttct ttgtcttcat ctgaaaaatc catagtacgg actgtattat tatattcaaa
     1861 agaatcttct acccatttag cacggtaatc cagcaacttc agtttatccg gagcatatcc
     1921 ctggaaaagc atttccgctg tcggaagaac ttctacgccg gatttaccgg cgatcagagc
     1981 tacattgaat tcacgtccgt caatataggc ttcggcaaaa agagatccaa ccaggttttt
     2041 tttgcgttca tttataagcg ctaaaagatc agttgatccg gtaagtttaa ctacgcaact
     2101 ttcatccagc cccacagatg catgttccca agcggctttc atgatatatg tgtcaaccga
     2161 agatgatccc gagcggatcc ctgcttcagt cacccattct ggcgtagcaa tacctgcctc
     2221 atgcattatt ttcttagcca gaggcttgtt cgatgtaaga aaaatagctt cggtgctaca
     2281 accagtataa ggaattttta gatagtccag gagagcaggt gcaaaataaa taaggctgcc
     2341 tttaccggca acagtctcca caagattgaa aacaacttcg gggcataaag aatttaaagc
     2401 ggtgatcgtt cgggttaaat caaaaacaaa gggaatcaaa acagggtcgt aaccaagctg
     2461 tagaagagct tccgcaatag tatctgcttg ccttaagcaa tcaagttcat cttcaccggc
     2521 atcgggtgca acatcactgt gtaaaattac tatttttttc attttcgcaa aatttaaccg
     2581 gattaggctg gtggttaatt atttatatta gtgcgccgca aagccgattt aataatggag
     2641 tttatcaatt catcgtagct cattcccaag agattgcaga gaatgggtaa gtccgaacgc
     2701 ttaggattta atccggcaag cgaattaact tccatgaaat gcggtgctcc acaagcatca
     2761 gagcgcaaat ccacacgacc agcatctttc aaatccaatc cgcgccaaag acacagcgat
     2821 atttccttgg cttgtttagc ttcatcatca tcaaccagaa tatattggac tagccgatca
     2881 taatgttcct tgttttcata ggaataagca tttttttctg ccgccggctt taaaatgacc
     2941 tctattgctc cgaggacttt agcatttttt ccggagccta aaatgccgac agtaaattcg
     3001 cggccgggaa gatatgtttc gactagtgcc ggctgtttaa aagtctgtaa aatatattta
     3061 caaattcgat acaattcttc acggttggtt atttttgaca gtgacgttat gcccttgcct
     3121 gttccttccg ctacgggctt ggcaaaaagt ggcaaaggaa gcttgacgtc ggcaatttct
     3181 gactcatagc tgactacagc aaaatcaggt gtgggtattc ccatatcgcg gagaacatgc
     3241 ttggtcatgc ccttgtggag agtaagggca tgaccaagtg gatcagaaaa agtataggga
     3301 atattgtatg cgtccagcaa agcgggaact tgcgcttcgc ggccaaagcc atgaaggcct
     3361 tcggcaacat tgaaaacaag atcccatcga tttcctgcaa caagctggcg agtcagcgct
     3421 tttacgttgc caattcgatc agcttgaaaa ccgttatcta taatcacctt ttcaatcgat
     3481 tcgattgtat caagaagatc gaattccgcc gtgtcttcga gattatatcc ttctttcaaa
     3541 tattcatcac gtaagtcata agttatgcca atttttaatt ttttaccggt gcgactcaat
     3601 attaactatc aaccccttac cataaatttc tgccaattta atgtatatcc ctcgattctg
     3661 cgtgattgtc gggatagcgg aaaagattac cttcgtaatt gcgtaacagc aaagcgcctt
     3721 tttcccatcc gatagcatat tcaggaagca aaggaatttt tccgccgccg ccgggcgcat
     3781 cgacaacata agtaggtaca gcataaccgg ttgtgtatcc gcgcataccc tggataattt
     3841 ccagcccctt actaatcggt gtccggaaat gagaagatcc gggaattggg tcacactggt
     3901 agagatagta aggacgaact cgaatttgca gctggccatg cactagtctt atcattgtgg
     3961 caacgtcatc attgataccg gaaagcagga cagtctgact tcctagcgga atgccggcat
     4021 cagccaggcg aatacaggca gcgctcattt cgggcgttaa ttcgtcagga tgagtgatat
     4081 gaatgctcag ccataaagga tgatattttt tcagcatagc ggtaagaacc ggagttattc
     4141 tttgtggcaa aacaaccggc actttagttc ctatacgcag aagttcaaca tgaggaattt
     4201 tgtgcaaccg cattaacagc caatccagct tctcatcaga caatgttaac ggatcgcctc
     4261 cggagagaag cacatctctg acgttttgat tgttttcaat atattctata gccttctccc
     4321 atttttcaaa gtcgacaaaa ttttgattac tacgttcgat aatccgggaa cgcgtgcagt
     4381 agcggcaata ggttgaacag gtgtcggtta ctaaaaaaag cacgcgatcg ggatagcgat
     4441 ggataatgcc gggtaccgga gaatcggcat cctcacatag aggatcttct tcttcacctg
     4501 cggtatgcaa acattcatct acaacgggaa caacgcagcg acgcagaggt gcagaaggat
     4561 cgtcaggatt aagcaggctg gcgtaataag gcgtaatgga aacgggcaat gctcccgagt
     4621 ggtgcatcat agcctgtcgt tcattatcag aaagattaag catctgggac aaggtgtcga
     4681 catttctgat cgcgttgcgc aactgccaat gccaatcgaa ccattcgaag cgagtaatgt
     4741 cgggataaaa acgattacgg aaaatacgcg atcggggtga atccttaaaa ggaaataaaa
     4801 gcctctccgg cttttgttta gccgggtatg gccggtgagt atctctgttt aatggaaaaa
     4861 attcatttac ttcattttca accttaacaa gaccggtcaa aagaccagga ggttcctcct
     4921 cctcattaaa aagggtctcg ttttccattt tgttcctcct tctggaagtt tttcttccac
     4981 taaatatttt tgtaattgaa aattacaaat cttataattg gaatgtacct gtactataaa
     5041 atttgtcaac actttttttc aaaaaatgac atttttttta cagatgaaac ttttccttaa
     5101 aataagtttc acggacgctc ttggcttttt cttgagccca tttttcaatt cccatctttt
     5161 ttatcaaaca taaattgaaa attttcgtgt tatgcggacg ctgtgaggcc atatccgcgt
     5221 aaggatgcag gtgaccgcag gggaaatcgg aacattcaaa acaaaacttt aattgctttt
     5281 aagaaatgca tttaaaaacc tgaccggact cagtcctttt
//
LOCUS       DASVIM010000050         4763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1300022, whole genome shotgun sequence.
ACCESSION   DASVIM010000050 DASVIM010000000
VERSION     DASVIM010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300022"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1419)
                     /gene="murD"
                     /locus_tag="VF305_00995"
     CDS             complement(76..1419)
                     /gene="murD"
                     /locus_tag="VF305_00995"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX7415741.1"
                     /translation="MDFTGKKIIIIGMGKTGIAVAKFLGKRGAKVVATDEKPFDQWGI
                     EFEQIAQEKWLEPGDYNARILTGASMVIPSPGVPPYNDLLVAALKKNIPVFSEIELAY
                     WFLKVPVIAVTGTNGKTTTATLLGEILKCSGKKTFVGGNIGNPLIEYVESSQKEDFVV
                     AEISSFQLQWIEKFRPFVSILLNVTSDHINYHGSFAEYRRIKTRLFENQTKADYAILN
                     AADSAQEGMAETIRAQISTFNSVNALPTGIFIKNDIIVFRMSGESEEQYPLSMIALPG
                     LHNVENVMAAIMAARFCGCSQENIIAAVAAFRGLPHRIEFAGEKNSVKFYDDSKGTNV
                     GSVMRALDTFAKPVILLLGGRDKDGDFKTLKPSLAIKAKKVILFGEARNRIESLIGGD
                     VPTLKKTKLREAIESAYKDAHSGDVILLSPGCASFDEFANYKERGDFFKEIVRNL"
     gene            complement(1427..2506)
                     /gene="mraY"
                     /locus_tag="VF305_01000"
     CDS             complement(1427..2506)
                     /gene="mraY"
                     /locus_tag="VF305_01000"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX7415742.1"
                     /translation="MIYHLLYPLHTTFSFFNVFRYITFRTIFASITALLICLVVGPWL
                     IRKLQSLQIDQQIREDGPQSHLVKKGTPTMGGVLIIFAVVISTLLWANFSVKYVWLIL
                     LVTVGYGLIGLVDDYHKLTGKNSKGISGKTRLAAEILIAVFVSMILYLKPGFNSQITI
                     PFFKTALPNLGWGYVLLSTFIIVGAANAVNLTDGLDGLAIGPAIICFATYVLFAYLAG
                     NVKVASYLQITYVSGAGELAVFCGALVGAALGFLWFNAYPAEVFMGDVGSLSLGGALG
                     TMAIITKQEILLAIVGGIFVVETFSVIFQVGYFKLTRGKRIFRMAPLHHHFELKGWAE
                     PKVIVRFWVISILLALMAISTLKLR"
     gene            complement(2524..3936)
                     /gene="murF"
                     /locus_tag="VF305_01005"
     CDS             complement(2524..3936)
                     /gene="murF"
                     /locus_tag="VF305_01005"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX7415743.1"
                     /translation="MLKATDGILISGAPKNTFRGISTDSRLVNKENLFIALKGEKFDG
                     HNFVQTALDKGAAGVLVHDETKNLWGVNKNVALIKVADTLTALGDLAQEWRRRFPIPV
                     IGLTGSSGKTTVKEMMAAIIGREKNVLKTEGNLNNLIGLPQTIFRITKEHELVILEMG
                     TNIRGEIKRLTQIAAPDIGLITNVGPAHLAGFGSVDVVRKEKGDLFLNMSPSGIVIVN
                     LDDEAVRIAAERWNGRRITFSMSPNADVTVKDIERNGVKGVRFNLVIGGNTQKVEMKI
                     AGIHHVYNAMAAAAAAWAAGIGPRTILEGLAAFQPVSGRMEMIKLQNGAYLLDDSYNA
                     NPVSVREALLTLKDLKNHHGGYVFLGDMLELGAAAEEMHRKIGILIATIGVNALFLQG
                     DFSAVTAAGAVDGGLPKQNIFFLSDSTDGIGYLKEHFKKGDWVLVKGSRRMKMEKIVA
                     QICEAFGGEKINGNNNQAVH"
     gene            complement(3977..>4763)
                     /gene="murE"
                     /locus_tag="VF305_01010"
     CDS             complement(3977..>4763)
                     /gene="murE"
                     /locus_tag="VF305_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HEX7415744.1"
                     /translation="RILEEVGLPALTYGIEGDSAIKAASYELSLAGIKAKINLAGENI
                     SIQTSLIGKFNLYNILAACAAATALLISPAVIKVGIENLSYVPGRLEKIDSLSDFIVL
                     VDYAHKADALKQVLQNLAEFRKKRIITVFGCGGNRDRGKRPLMGEAAAKYSDLTIVTS
                     DNPRWEDPLDIIAEIETGIDQNKVKKVAIDQLKIGNSVHSYMVIPDRRKAIETAINRA
                     QKGDIVLIAGKGHEDYQIIGTKKMPFDDRIVATQALELRSQVQ"
BASE COUNT         1346 a         1197 c          865 g         1355 t
ORIGIN      
        1 agtcaccaaa atcaaagtaa ctaaaagtaa aataatgtcc ggcaatttat tttcattttt
       61 gactgcggca tccatctaaa gattcctcac tatctcttta aaaaaatcac cccgttcttt
      121 ataattggca aattcatcaa agctcgcaca acccggcgag agtaaaatga catcaccgga
      181 atgagcatcc ttataagcgc tttcaatagc ttcccgtaat ttcgtttttt ttaacgtcgg
      241 cacatctcca ccgattaacg actcaatacg gttgcgtgct tcaccaaaca aaattacttt
      301 tttggctttt attgcgagcg atggcttcag ggttttaaaa tcgccgtctt tatcgcgtcc
      361 ccccaaaagc aaaattacgg gcttcgcaaa tgtatccagt gcccgcataa cggaaccaac
      421 attcgttccc ttggaatcat cgtaaaattt gaccgaattt ttctcacccg caaattcgat
      481 tcggtgcggc aatccgcgaa aggcggcaac agccgcaatg atattttcct gactacaacc
      541 acagaagcga gccgccatta tcgccgccat cacattctcc acattatgca aaccaggaag
      601 agcaatcatg ctcagaggat attgctcctc gctctcaccg gacattctaa aaacgataat
      661 atcgttctta ataaatattc ctgttggcag cgcattgacc gaattgaacg tagaaatttg
      721 tgcacgaatg gtttcggcca ttccttcctg cgctgaatct gcggcattaa gaattgcata
      781 atcagccttt gtctgattct cgaacaatct cgttttgatg cggcggtact ccgcaaaaga
      841 accatgataa ttaatgtgat cgctagtaac attcaaaagt attgacacaa agggccgaaa
      901 tttttctatc cactggagtt ggaagctgct gatttcagcg acgacaaaat cctctttctg
      961 tgagctttcc acatattcaa tcaaaggatt gccaatgttc ccgccgacaa atgttttctt
     1021 tcccgagcat ttcaaaattt cacccaacag ggtcgccgtt gttgttttac cattggtgcc
     1081 ggttacagcg attactggaa cttttaaaaa ccagtaagcc agttcgattt cactgaaaac
     1141 aggtatattc ttttttagcg ccgcaactag caaatcgttg tagggaggaa cgccgggaga
     1201 agggataacc atgcttgcac ccgttaaaat acgggcgtta taatcaccgg gttccaacca
     1261 tttttcttgg gcaatttgtt cgaactcaat accccattga tcgaacggct tttcgtccgt
     1321 ggccactact tttgctcctc gttttcctaa aaacttggcg acggcgattc ccgttttgcc
     1381 cattccgatg attattattt ttttaccggt aaaatccatg cttcctctaa cgtaacttca
     1441 gtgtgcttat ggccattaag gccaataaaa tagaaatgac ccaaaaccgc acaattactt
     1501 tcggctccgc ccaacccttc aattcgaaat ggtgatgcag gggcgccatc cggaaaatgc
     1561 gtttccctct agtcagcttg aaatagccaa cttgaaaaat gactgaaaat gtttccacaa
     1621 caaatatgcc gccgacaata gccagcaata tttcttgttt ggttataatc gccatcgtgc
     1681 ctaacgctcc acctagcgaa agggagccga catctcccat aaaaacttcc gcaggataag
     1741 cattaaacca aaggaaacca agggctgcgc ctacaagagc tccacagaat accgccaatt
     1801 caccggcacc ggaaacgtag gtaatctgca aatacgaagc aactttcacg tttccggcaa
     1861 gataggcaaa aaggacatag gtggcaaaac agataattgc gggaccgata gccagaccat
     1921 ccaagccgtc tgtaagattt acggcattag ctgcacccac gataataaac gtagaaagca
     1981 ggacatagcc ccaaccaaga ttaggcaatg ccgttttaaa aaatgggatt gttatttgcg
     2041 agttgaaccc gggcttcaaa taaagaatca tgctgacgaa aacagcgatc aatatttccg
     2101 cggctagcct tgttttaccg gaaatgcctt tggaattctt gccggttagc ttatggtaat
     2161 catcaacaag cccgataagc ccgtaaccaa ctgtcaccag caatatcaac cagacatatt
     2221 taacggaaaa gtttgcccaa agcagagtcg atatcacgac ggcaaaaatg attaatactc
     2281 cccccatcgt gggagtgcct ttctttacta gatggctttg cggcccgtct tctctgattt
     2341 gctgatcgat ttgcaggctt tgtaattttc gaattagcca tgggccaaca accaggcaaa
     2401 tcaaaagggc agtgattgat gcaaatatcg tccggaaagt aatataacga aatacgttaa
     2461 aaaacgaaaa cgttgtatgt aaaggatata ataaatggta aatcaattct gattcactcc
     2521 ttattaatga accgcttggt tgttatttcc gttaattttc tcaccgccaa aagcctcaca
     2581 gatttgagca acaatttttt ccatcttcat ccggcgcgaa cccttcacca aaacccagtc
     2641 tccttttttg aaatgctctt ttaagtaacc aataccgtca gtgctatcag acaaaaagaa
     2701 aatattttgt tttggcagtc ctccatccac agcgccagca gcggtaactg cagaaaaatc
     2761 accctgaaga aaaagggcat tgacacctat agtcgctatc aagattccaa ttttacgatg
     2821 catttcttca gccgctgctc ccagttccag catgtcgcct aaaaatacat aaccgccatg
     2881 gtggttcttt aaatctttca acgtcagcag agcttcccgc actgaaacag ggttggcgtt
     2941 gtacgaatca tccaaaaggt aagcgccatt ttgcagtttg atcatttcca tgcgtccact
     3001 aaccggctgg aatgccgcca aaccttctaa tatcgtcctt gggccgatgc ccgcagccca
     3061 agctgctgcc gctgccgcca tagcattata aacatgatga ataccggcaa ttttcatttc
     3121 tactttttgc gtattgccgc cgatgactaa gttaaagcgc actcctttaa caccatttct
     3181 ttctatatct tttacagtga catcggcatt aggactcatg ctaaaagtaa ttcgccggcc
     3241 attccatctt tcggcagcaa ttctaacagc ttcatcatca agattaacga taacaatgcc
     3301 cgagggcgac atattaagaa ataaatcacc cttttccttt ctaacaacat ctacggaacc
     3361 aaatcctgcc agatgcgctg gtccaacgtt cgtgatcaaa ccaatatccg gcgcggcaat
     3421 ttgcgtaagt ctctttattt ctcccctaat attcgtaccc atctctaaaa tcaccaattc
     3481 atgttccttg gtaatgcgaa atatcgtctg cggcagcccg atcagattat ttaaatttcc
     3541 ctcagttttt agaacatttt tctcccggcc gatgatcgcc gccatcattt ccttgaccgt
     3601 tgtctttccg gaggaaccag tcaatccaat aaccggaatg ggaaaccgtc ttctccactc
     3661 ctgagctaaa tctcccagcg cggttaatgt atcggcaacc ttgattaggg cgacattttt
     3721 attcactccc cataaatttt tcgtctcatc atgcaccaat actccggcgg cgcctttatc
     3781 cagcgctgtt tgcacaaaat tatgaccatc aaacttttca cctttcaatg caataaaaag
     3841 attctcttta tttaccagcc gcgaatccgt ggaaattcca cgaaatgtat tctttggtgc
     3901 cccggaaatt aaaattccgt ccgttgcttt cagcacctcc tctaatgaaa atattggtga
     3961 atcatttttt atcaatttat tgcacttggc tcctcaattc taaagcttgc gtggcgacaa
     4021 tccgatcatc aaacggcatt ttttttgttc caataatttg ataatcttca tgtcctttac
     4081 ccgcaatcaa aacaatatct cccttttgtg ctctgttgat tgctgtctca atcgcttttc
     4141 tccgatcagg gattaccata taagaatgaa cactattgcc gattttcagc tgatcaatcg
     4201 ccaccttttt taccttattt tgatcaattc ctgtttcgat ttccgcaatg atgtccagcg
     4261 gatcttccca gcgcggatta tccgaggtaa caatagttaa atcactgtat ttggcggccg
     4321 cttcacccat gagcggtctc ttgccgcgat ctcgatttcc tccacagccg aaaacagtaa
     4381 taattctttt ttttctgaat tccgccagat tttgcagtac ctgctttaaa gcatccgctt
     4441 tgtgcgcgta atcaacaaga acaatgaaat cggatagaga atcaattttc tctaagcgtc
     4501 cgggcacata agataaattt tctattccca ctttaattac cgctggtgaa attagtaatg
     4561 ctgtcgcggc ggcacaagca gcaagaatat tataaaggtt gaacttgccg ataagcgaag
     4621 tctgtatgga aatattttcc cccgccagat taattttggc ttttattccc gccaaggaca
     4681 attcataact tgccgcttta attgcgctgt cgccttctat gccataagtt aacgccggta
     4741 atcctacctc ttccaaaatc ctt
//
LOCUS       DASVIM010000051         8212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_446868, whole genome shotgun sequence.
ACCESSION   DASVIM010000051 DASVIM010000000
VERSION     DASVIM010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446868"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="VF305_01015"
     CDS             complement(<1..137)
                     /locus_tag="VF305_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HEX7415745.1"
                     /translation="MEAKAVAKYIRMSPQKVRLVVDLVRGKKVQEAKNILLYTRKYAA
                     G"
     gene            complement(179..493)
                     /gene="rpsS"
                     /locus_tag="VF305_01020"
     CDS             complement(179..493)
                     /gene="rpsS"
                     /locus_tag="VF305_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX7415746.1"
                     /translation="MARSIKKGPYIEERLLTRVRKMETEGSKNVIKTWSRRSTITPEL
                     IGYTFAVHNGKKFIPVYVTENMVGHKLGEFAPTRTFYSHSGDRKTKVRTTTPPTTTTT
                     TT"
     gene            complement(500..1321)
                     /gene="rplB"
                     /locus_tag="VF305_01025"
     CDS             complement(500..1321)
                     /gene="rplB"
                     /locus_tag="VF305_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX7415747.1"
                     /translation="MGIIKYKPTSAGRRFQSVADFAEITSAEPVKSLLKPLKKTGGRN
                     CYGRITARHIGGGHKRKYRIIDFCRSKTDIPAKVAAIEYDPNRTARIALLNYSDGEKR
                     YIVAPAQVNVGDTIISVEKADIKPGNAVPLKYIPLGSLIHNLELKVGRGGQLIRSAGA
                     YGQLMAKEGSYAQVRLPSGEVRKVFIECMATLGQVGNNDHENISIGKAGRTRWFGKRP
                     HVRGVAMNPVDHPMGGGEGKSSGGRHPCTPWGVPTKGHKTRKNKKTDKYIVKRRG"
     gene            complement(1346..1633)
                     /gene="rplW"
                     /locus_tag="VF305_01030"
     CDS             complement(1346..1633)
                     /gene="rplW"
                     /locus_tag="VF305_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX7415748.1"
                     /translation="MEVYQIIKKILVTEKSTAAREKLNKYFFEVDRKANKMEISKAVE
                     KLFKVKVADVRVLHVLGKKKRLGRIIGTKSSWKKAIVTLAAGSRIEIAESV"
     gene            complement(1633..2256)
                     /gene="rplD"
                     /locus_tag="VF305_01035"
     CDS             complement(1633..2256)
                     /gene="rplD"
                     /locus_tag="VF305_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX7415749.1"
                     /translation="MAVADVFDIKKKKVAQVDLNDAVFGAEVNEAIVYDVIKMQLASR
                     RAGTASTKTRSDVRGGGKKPWRQKGTGRARAGTTRSPIWRGGGIVFGPHPRDYSYSVP
                     KKVRKKAIICALSMKLKQNKMVILRDFPMEKISTGIFKSVVDLFGLKKALFVLDNNNE
                     VLQKSSRNIKNVKMIRSEGINVYDILNHEHLILLEPAVKKIEGALLA"
     gene            complement(2278..2913)
                     /gene="rplC"
                     /locus_tag="VF305_01040"
     CDS             complement(2278..2913)
                     /gene="rplC"
                     /locus_tag="VF305_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX7415750.1"
                     /translation="MMNKGIIGKKLGMTQVFADDGAAVGVTAIEVDPSVIVQVKTKDK
                     DGYDALQLGYGRIKQKNVTKPLQGHLNKANKGFFRVLKEFSAEPGKHEVGQEIAADIF
                     QAGDFVDVVGTSKGKGFQGVVKRHGFHGGRATHGSMFHRAPGSIGASADPSRVFKGTK
                     MGGHMGNVRKTIQNLQVWQVRPDRNLLLLRGSIPGSKNGFVLIKQAKKKSA"
     gene            complement(2997..3305)
                     /gene="rpsJ"
                     /locus_tag="VF305_01045"
     CDS             complement(2997..3305)
                     /gene="rpsJ"
                     /locus_tag="VF305_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX7415751.1"
                     /translation="MKDQKIRIRLKAYDYKLLDRSVEEIVETAKRTGARVAGPIPIPT
                     EINKYCVNRSPHVDKKSREQFEIRTHKRLIDIVEPTQATVDALMKLDLSAGVDVEIKA
                     "
     gene            complement(3331..4521)
                     /gene="tuf"
                     /locus_tag="VF305_01050"
     CDS             complement(3331..4521)
                     /gene="tuf"
                     /locus_tag="VF305_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX7415752.1"
                     /translation="MAKKKFERTKPHLNIGTIGHVDHGKTTLTAAITKHLAKKGFAEF
                     RPFDSIDNAPEEKERGVTINISHVEYETEKRHYAHVDCPGHADYIKNMISGAAHMDGT
                     ILVVAASDGPMPQTREHILLARQVQVPYIVVYLNKVDLVDDPELLDLVELEMRELLNQ
                     YEFPGDDIPIIRGSALKALETDDSNSADAKSIWELLTAIDNIPQPKRDVQKPFLMPVG
                     DVFTISGRGTVVTGRVDRGIVKVGEEVEIIGIRPTIKTVVTGVEMFRKTLDEGRAGDD
                     VGLLIRGIKREEVERGQVVAKPGSITPHTKFEAAVYILTKEEGGRHTPFFSGYRPQFY
                     FRTTDVTGVATLPEGVEMVMPGDNVKMTVELITPIAMEEQLRFAIREGGRTVGAGVVS
                     KIIE"
     gene            complement(4588..5058)
                     /gene="rpsG"
                     /locus_tag="VF305_01055"
     CDS             complement(4588..5058)
                     /gene="rpsG"
                     /locus_tag="VF305_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX7415753.1"
                     /translation="MPRRREIEKRDVLPDPKFNNKLVAKFVNSLLKRGKKSIAESILY
                     GAFDIMEKKLKEQPVELFEKAVNNVKPVIEVKSRRVGGSTYQVPTEVMPARRTALAIR
                     WLIMNAQERTEKTMREKLAGELIDAANNRGGAIKKKESVHKMAEANKAFAHYKF"
     gene            complement(5073..5444)
                     /gene="rpsL"
                     /locus_tag="VF305_01060"
     CDS             complement(5073..5444)
                     /gene="rpsL"
                     /locus_tag="VF305_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX7415754.1"
                     /translation="MPTISQLIRKGRVKVQKKTNTPALANSPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTSGYEVTSYIPGVGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGTL
                     DTVGVQDRKQSRSKYGAKKPS"
     gene            complement(5555..>8212)
                     /locus_tag="VF305_01065"
     CDS             complement(5555..>8212)
                     /locus_tag="VF305_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7415755.1"
                     /translation="EARVLMMSTNNILSPANGKPIIIPSQDIVLGIYYLTRAKNGVKG
                     EGMIFAGPDEVRSAIDAGAVDLHARIKVRIDSELKDTTVGRIILSEIVPEEIDFDTIN
                     KLMNKKELANLIDYCYRLCGDKTTVLLADRLKDLGFKYATISGLSFAIGNMIIPENKK
                     HIVLQADKDVLKIQKQYMDGLITDGERYNKVIDIWAQSTEKIAAEMLKGISTEEVPAA
                     DGKKRKIESFNPIYMMADSGARGSAVQLRQLAGMRGLMAKPSGEIIETPITANFREGL
                     TVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDCIITEQDCETVDGIYVSAL
                     MEGGEIIETAGERVLGRVALQKIKDPFTGEVIVKANEAIDEALAEKIDRAGIERVNIR
                     SVLTCRSKHGVCTMCYGRDLAHGHLVNIGEAVGIVAAQSIGEPGTQLTMRTFHIGGTA
                     KFDEHSTLEARHDGVIKFVNLNTVKTRENDFVVMNRHGEVHVLDEQKRSRGKYTVPYG
                     AHLKVSNHSSAKRGQLIAEWDPFSIPILAEVDGTIKFGDIIEGKTMQEQVDEVTGLSR
                     KVIVEFKGGDLRPRVSIKDSKGKTAKLASTGSGARYFLSVGVNLVVSEGDQVRAGDIV
                     AKIPRETTKTKDITGGLPRVAELFEARKPKDFAVISEIDGVVSYGKDAKGKRKIVVTP
                     EVGEEKEYLIAKGKHISVQEGDRVTPGDALMDGVNNPHDLLAIKGEKELARYLVDEVQ
                     EVYRLQGVKINDKHMEVIVRQMLRRIRVSDPGDTAFIADEQVERYRFQEENEKVIAQG
                     GHPAIGEPLLLGITKASLSTQSFISAASFQETTRVLTEASLSGKTDYLRGLKENVIMG
                     RLITAGTGLVMYRKLGIKVEDGNSEANIE"
BASE COUNT         2057 a         2090 c         1525 g         2540 t
ORIGIN      
        1 atacctgccg catactttct ggtgtaaaga aggatatttt tggcttcctg caccttcttc
       61 cctcttacta aatcaaccac caacctgact ttctgcggcg acatccttat atattttgca
      121 actgcttttg cttccatgtt aaatcaactc caaaccttat caggcatagc ttaaagaact
      181 aagtagtagt ggtggtggta gtaggaggag tagtagtacg gacttttgtt ttccggtcac
      241 ctgaatgcga ataaaaagtc ctcgtcggcg caaactcgcc gagcttatgc cctaccatat
      301 tctccgtcac ataaaccgga atgaattttt tcccattatg tacggcaaac gtgtaaccaa
      361 tcagttccgg tgttatggtt gaacgccgcg accaagtctt gatgacattc ttgctgcctt
      421 cggtctccat tttcctgacc cgggtcagca acctttcttc aatgtatgga ccttttttga
      481 tcgaacgtgc cacgcttttt taacctcttc tcttcacaat atatttatca gtttttttat
      541 tttttctcgt cttgtgacct tttgtcggca caccccaagg cgtacacgga tgcctgccac
      601 ctgatgactt tccttcgcca ccacccatcg gatggtcaac cggattcatc gctacgccgc
      661 gaacatgagg ccttttacca aaccagcgag ttcggcctgc tttgccgata ctgatatttt
      721 catggtcgtt gttgcctacc tgcccaagcg tggccataca ctcaataaat actttgcgca
      781 cttcacctga tggaagcctt acctgagcgt aactgccttc cttggccatt aactgaccgt
      841 aagctccggc agaacgtatt aactgcccac cgcggccaac ctttaattcc agattatgaa
      901 ttaatgagcc caggggtatg tactttaaag gaacagcatt accgggtttg atatccgcct
      961 tctcaacact gattatagta tctccgacat tgacttgtgc cggtgcaaca atgtatcttt
     1021 tttcaccatc actataattg agcagcgcta ttcttgccgt cctgttcgga tcatactcga
     1081 ttgcagcaac tttcgccgga atgtccgttt tacttcggca aaaatctatt attcgatatt
     1141 tgcgtttatg accgccgccg atatgccgag ctgtaattcg tccataacag ttacggcccc
     1201 cggttttctt taacggtttt aagaggcttt ttaccggctc agccgaagta atctctgcaa
     1261 aatcagcaac actctgaaat cttctgcccg ctgatgtcgg tttatattta ataattccca
     1321 ttagctcaat ccttataact agttcttata cgctttcagc aatttcaata cgacttccgg
     1381 ctgccaaagt tacgatggcc tttttccaag aactcttcgt ccctataatc cggccaagcc
     1441 tcttcttttt tcctaaaacg tgcagaacac gaacatcagc gactttgact ttaaacagct
     1501 tttccactgc tttgctgatt tccatcttgt ttgccttgcg gtcaacttca aaaaaatatt
     1561 tattcaattt ctcccgcgct gcagtgctct tttctgtaac cagtattttc ttaataatct
     1621 gatatacttc cattatgcca gcaatgctcc ttcaatcttc tttacagcgg gctcaagaag
     1681 gattaaatgc tcatgattta aaatatcata aacattaata ccctctgatc tgatcatctt
     1741 aacgtttttt atattacgcg aagatttttg taaaacttca ttattattat ctaaaacaaa
     1801 aagcgccttt ttcaagccga aaagatcaac gacgctttta aatataccgg tactgatctt
     1861 ttccattgga aaatctctca ggatcaccat cttattttgt ttgagcttca tgctcagcgc
     1921 gcatattatg gcttttttac gtactttttt cggtacacta taagaataat ctcggggatg
     1981 cggtccaaaa actatcccgc cgcctcgcca aataggtgac cttgttgtcc ccgctctggc
     2041 ccggcctgtt cctttttggc gccaaggctt tttcccgccg ccacggacat cacttctcgt
     2101 ttttgtggaa gcagtaccgg cacggcgcga cgccaattgc attttaataa catcataaac
     2161 aattgcttcg ttgacttcag cgccaaatac ggcgtcattt aaatcaactt gagcaacttt
     2221 cttcttttta atatcaaaaa catctgcgac tgccatgttt ataactccgg atgtgattta
     2281 agcacttttt ttcttggctt gtttaatcaa cacaaatcca tttttactgc cggggatcga
     2341 acctcttaac aagagcaaat ttctatcggg acggacttgc caaacttgca aattctggat
     2401 agttttacgc acattgccca tatgaccgcc catttttgtg cccttaaata ctcgcgacgg
     2461 atcagcgctg gcaccgatag atcctggtgc tcgatggaac atggaaccgt gtgtggcccg
     2521 gccaccgtgg aaaccatgcc tcttgacaac gccctgaaaa cctttgcctt tggaagtccc
     2581 aactacatca acaaaatctc ccgcttgaaa aatgtctgcc gcaatttcct gtccaacttc
     2641 atgcttacct ggttccgcgg aaaattcttt aagaactcgg aaaaaacctt tattcgcttt
     2701 atttaaatga ccctgcagcg gtttggttac attcttttgt tttatccgcc cgtaaccaag
     2761 ctgaagagca tcgtatccat ccttatcttt agtttttacc tgcacgataa cggacggatc
     2821 gacttcaata gcagtgacac cgacagcggc tccgtcatct gcaaatactt gggtcattcc
     2881 caactttttt ccaattattc ccttgttcat catcatccaa ctttttaata aaataaaacc
     2941 aatatttgaa gaatggcagt aattcgttac catttcctag cacttgaacc gtatgcctac
     3001 gcttttattt ccacatcaac tccggcggaa agatctaatt tcatcaaagc atccaccgtg
     3061 gcttgagtag gctcgacgat atcaatcaat cttttatgtg ttcgaatttc aaactgctcg
     3121 cgggattttt tgtccacatg aggtgagcgg tttacacaat acttgttgat ttccgtggga
     3181 ataggaatag gaccggcaac acgtgctccg gtcctttttg ccgtttccac tatttcttct
     3241 acagagcgat ctaaaagttt ataatcgtaa gctttgagac gaattctaat cttttggtct
     3301 ttcatttatc ggataaccta ttccctattt ctattcaata atcttgctta cgactccagc
     3361 gcctacagtt cttccaccct cacgaatagc gaatctcagt tgttcttcca tcgcaatggg
     3421 cgtaatcaac tctaccgtca tttttacgtt atcaccgggc attaccattt ctacgccttc
     3481 aggaagggtt gccacaccag tcacatccgt tgttcggaaa tagaactgtg gccggtaacc
     3541 ggaaaagaat ggtgtatgtc tgccaccttc ttccttggtt aaaatgtaaa cagctgcttc
     3601 aaatttcgta tgcggagtaa tggaaccggg cttagcgaca acctgccctc tttccacttc
     3661 ctcgcgctta attcctctta taagcaaacc aacatcatca ccggcgcggc cttcatcaag
     3721 ggtcttgcgg aacatttcca caccggtaac aacagtctta atggttggtc taataccgat
     3781 aatttccact tcctcgccaa cctttacaat acctctgtct accctgcctg taacaaccgt
     3841 tccacgaccg gaaatagtaa atacatcacc aaccggcatc aggaaaggct tttgtacatc
     3901 gcgtttaggc tgtggaatgt tatctatggc agtcaaaagt tcccagatcg attttgcgtc
     3961 ggctgaatta gaatcatctg tttccaaagc tttgagtgct gaaccacgaa taatgggaat
     4021 atcgtcaccg gggaattcat attgatttaa aagttctcgc atttcaagtt ccactagatc
     4081 cagaagttca gggtcatcaa ctaaatctac tttattaaga taaacaacaa tgtagggaac
     4141 ttgaacctga cgggcaagga ggatgtgttc acgagtttgc ggcatcggac catcggaggc
     4201 ggcaacaaca aggattgttc catccatgtg tgcggcgccg gaaatcatat ttttaatata
     4261 gtcagcgtga ccggggcaat ccacgtgagc gtaatgcctt ttttctgttt cgtattccac
     4321 gtgggagata ttaatcgtaa ctccgcgctc tttttcttcg ggagcattat caatagagtc
     4381 gaaaggcctg aattccgcaa agcctttctt cgccaaatgt ttggtaatag cggctgttaa
     4441 tgttgtcttg ccgtgatcaa cgtgaccgat tgttccgata tttaaatgcg gcttagtcct
     4501 ttcaaatttt tttttagcca tgtcaattta cctccttaac tttgaatggt tggttctctt
     4561 gtcgtttaag tattatttcc ttatttatta aaatttataa tgagcgaaag ctttatttgc
     4621 ttccgccatc ttatgcacac tttctttttt cttgatcgcc cctccgcggt tgttggcggc
     4681 gtcgattaac tcaccggcca gtttttctct catagttttt tccgtgcgct cctgggcatt
     4741 cataatcaac cagcgaattg ccagcgctgt gcgtcttgcc ggcataactt ccgtaggcac
     4801 ttgataagta gaaccaccga cgcgtcttga ctttacttca attaccggct ttacattatt
     4861 taccgctttt tcgaaaagtt caaccggctg ctcttttaat tttttttcca taatgtcaaa
     4921 agcgccgtaa agaatacttt cagcaatgct ttttttgccc ctttttaaca aggaattaac
     4981 aaattttgcg accagtttat tattaaactt aggatcgggc aaaacatccc ttttttcgat
     5041 ttcccgtctt ctcggcattc aattatcccc gtctaacttg gcttcttagc tccgtatttt
     5101 gacctactct gtttacggtc ttgtacccct acagtatcga gcgtacctct cacaatatga
     5161 taccttactc cgggtaaatc cttaactcta cccccacgta ccagtaccac ggaatgttcc
     5221 tgcagattgt gcccaacacc tggaatataa gaagtaactt cataaccgct ggtgagacga
     5281 accctcgcca ctttccgcaa agctgaatta ggtttcttag gagtcgtcgt atatactctg
     5341 acgcaaacac cgcggcgttg aggcgaattc gccagcgccg gtgtatttgt cttcttctgt
     5401 actttgactc ggcctttccg aatcagttga cttatcgtcg gcatctttcc tcccggacta
     5461 tatttccgcc acatcttttg tgagcagcag caaatcgcgg taactatcaa ttcaagccat
     5521 ttatgtcaag cattttctga acttttttat aaatttattc tatattcgcc tcggaattgc
     5581 catcctcaac cttaatacct aacttcctgt acattaccaa acccgtacct gccgttatca
     5641 gtcggcccat aatgacgttt tctttcaggc ctcttaaata atcagtctta cccgacagag
     5701 aggcttcagt cagcaccctg gtcgtttcct ggaatgatgc agcggaaatg aaactctggg
     5761 tactgagcga tgctttggta atacccaaaa gcaatggctc gccaatagcc ggatggcctc
     5821 cttgagctat gactttttcg ttttcttcct ggaagcggta acgttcgact tgctcatcgg
     5881 cgataaaagc cgtatcgcca ggatcgctca cgcgaatccg gcgaagcatt tgacgaacaa
     5941 ttacttccat atgtttatcg ttaatcttaa caccctgcaa acggtaaact tcttgcactt
     6001 catcaaccaa gtatctggcc agttcctttt cacctttaat ggctaaaaga tcatggggat
     6061 tgttaacgcc atccatcaaa gcatcaccgg gcgtaacacg atcaccttcc tgaacactga
     6121 tatgcttacc tttggcaata agatattctt tttcttctcc aacttccggc gttacaacta
     6181 tcttgcgctt acctttggca tccttgccgt aagagacaac accgtcgatt tcactgatga
     6241 cggcaaagtc tttaggttta cgggcttcaa ataattctgc cacacggggc aaacctcccg
     6301 tgatatcctt tgtttttgtt gtttcgcgcg gaatttttgc gacaatgtca ccggcgcgaa
     6361 cctgatcacc ctcggacaca actaagttta ctccgactga caagaaataa cgtgctccag
     6421 aaccagtact ggcaagtttt gctgttttcc ctttgctatc tttgatcgaa actcgcggcc
     6481 ttaaatcacc acctttaaat tcgactataa ctttacgcga taaaccggtg acttcatcaa
     6541 cttgttcttg catcgtttta ccttcgatga tgtcaccgaa tttaattgtt ccatccactt
     6601 cagccaaaat cggtatagag aaaggatccc attcggcaat taattgtccg cgtttggcag
     6661 agctatgatt gcttactttc aaatgggcgc cataaggcac ggtatacttg ccccgactac
     6721 gtttttgttc atccagaaca tggacttcac catggcggtt catcacaacg aaatcatttt
     6781 ctcgagtctt cactgtattt aaattaacaa acttaatcac accatcatga cgcgcttcca
     6841 gagtggaatg ttcgtcaaat ttcgccgtac caccgatatg gaacgttctc atggtcaact
     6901 gcgtacccgg ctcaccaatt gattgagccg caacgatacc gaccgcttca ccaatattaa
     6961 ccagatggcc gtgagccaaa tcacggccat agcacatggt gcaaacgcca tgcttggaac
     7021 ggcaagttaa aactgatcgt atattaaccc gttcaatacc ggcacgatcg atcttttcag
     7081 ccaaagcctc atctattgcc tcattggctt taacgatgac ttcaccggta aagggatcct
     7141 taattttttg taaggcaact cgacccagca cacgctcacc ggctgtttcg ataatttcgc
     7201 cgccttccat aagtgcggaa acataaattc catccactgt ttcacaatct tgctcggtaa
     7261 taatgcagtc ttgcgccaca tccactaacc ttctggtcag ataaccggag ttcgctgttt
     7321 tcaatgccgt atcggccaaa cctttacgtg ctccgtgggt ggaaataaag tattgaagaa
     7381 cagtcaagcc ttcgcggaaa ttagctgtaa tcggcgtctc gataatttca ccggagggct
     7441 tggccatcaa gccgcgcata ccggccaact gtcttaattg tactgccgag cctctggcgc
     7501 cggaatcggc catcatatag atggggttga aactctcaat tttacgtttc ttgccgtcgg
     7561 cagcgggaac ttcttcggtg ctgatgccct ttaacatttc cgcggcaatt ttttctgtgg
     7621 attgcgccca aatatcaata actttattgt aacgctctcc gtcggtaatc aaaccgtcca
     7681 tatactgttt ttgtatcttc aacacatcct tgtcggcctg cagaacaatg tgttttttat
     7741 tttccggaat aatcatgttg cctatagcaa atgaaagccc ggaaatagtc gcatatttaa
     7801 agcctaagtc cttgagtcta tcggccaaaa gaacagttgt cttgtctcca cacaaacgat
     7861 aacaatagtc gattaagttg gccagctcct ttttattcat taacttattg attgtatcga
     7921 aatcaatttc ttcgggaact atttctgaaa gtataattct tccaactgtg gtatctttta
     7981 actccgaatc aatgcgcact ttaatacgtg catgcaaatc gactgctccc gcatcaatag
     8041 ccgaacgcac ttcgtctggt cctgcaaaaa tcatcccttc gcccttaact ccattctttg
     8101 ctctggtcag ataataaatg cccaaaacga tatcttgact gggaatgata atgggtttcc
     8161 cgttggccgg agaaagaata ttgttggtgg acatcatgag gacacgggct tc
//
LOCUS       DASVIM010000052         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1129778, whole genome shotgun sequence.
ACCESSION   DASVIM010000052 DASVIM010000000
VERSION     DASVIM010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129778"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VF305_01070"
     CDS             <1..1008
                     /locus_tag="VF305_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7415756.1"
                     /translation="ADCNLAALKMFRGTREQLIGHPTWEFMPEFQPDGLLSTDMAKEK
                     IRKALKGDTLFLEWRFIRCDGSIFDAELTFNIITLKGEKYFQAIIRDITERKQAAEQM
                     KKLTQERERSNTDLAQFAYVASHDLQEPLRMVTSFVQLLQHRYQGKLDKDADEFISYA
                     VDGASHMQRLINDLLKYSRVGTHGKTLEPTNFNIVISHALSNLSKTIKENNAIINIDH
                     LPTISADYMQLVQLFQNLISNAIKYHSKKQPEILIRAINKGDFWLFSVRDNGIGIDKE
                     YNDKIFTIFQRLHNKSKYPGTGIGLSICKKIVERHGGRIWVESKPGQGSTFYFTIPSK
                     Q"
     gene            1021..1461
                     /locus_tag="VF305_01075"
     CDS             1021..1461
                     /locus_tag="VF305_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7415757.1"
                     /translation="MVNDKQQKMIDILLIEDNPGDVILIKEAFKEGQTCNKLTVVGDG
                     EAALSFLRKEGAYKKSSCPDLVLLDLNMPRKDGREVLAEIKTDPALKHIPVVILTSSE
                     AQQDIINAYNLQANCYITKPVDLKQFFNVVKLIEDFWLKTVKLP"
     gene            1471..>2368
                     /locus_tag="VF305_01080"
     CDS             1471..>2368
                     /locus_tag="VF305_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HEX7415758.1"
                     /translation="MFDKPIKILLFEDNLGDARLFAETIKEIPSQRYEIIHVNSLAEG
                     LTALGNKTADIILLDLGLPDSQHLDTLIKLKSQVLGVPIVILTGNDDEEIALRAVQQG
                     AQDYLVKQKINADVLSRVICYSIERQKLQDELRSLALIDDLTGLYNRRGFFTLAEHYL
                     KLAERMHKNFYLIFSDIDDMKKINDAFGHLQGDLFLKKTAEILRYSFRNSDIIARIGG
                     DEFVVLIPETTDYNAKSAITRLQENIKKYNKKEKKNLSLSMGIANFHPNLKFSLQDLL
                     DKADKHLYEEKKKKNMKNKPANN"
BASE COUNT          808 a          466 c          438 g          656 t
ORIGIN      
        1 gccgattgta acttagctgc attaaaaatg tttcgcggca ctcgcgagca gcttatcgga
       61 catccgacat gggaatttat gccggaattt cagcctgacg gccttctttc cacggacatg
      121 gccaaagaaa aaatacgaaa agctttaaaa ggtgatacgc tttttctgga atggcgtttt
      181 atccgctgcg acggaagtat atttgatgcg gaattaacct ttaatatcat caccttaaaa
      241 ggagaaaaat actttcaagc cattatcaga gacattacgg aacgcaaaca agcagccgag
      301 caaatgaaaa aattgactca agaacgagaa cgttccaata ccgacttggc ccaatttgct
      361 tatgttgctt cacatgatct acaggaacca ctgcgcatgg taactagttt tgtccagtta
      421 ctgcaacaca gatatcaagg gaaactggat aaggatgccg atgaattcat atcttatgcc
      481 gtcgatggtg catcccatat gcaacggtta atcaatgatc tcctaaaata ttcgcgcgtg
      541 ggcacacacg gcaaaacttt ggaaccgacc aatttcaaca tcgttatcag ccatgcctta
      601 tctaacttaa gcaaaacgat taaagaaaac aacgctataa tcaacataga tcatttaccg
      661 acaatttcgg ccgattatat gcaactggtc caactctttc agaatttaat cagtaatgcg
      721 ataaaatatc atagcaagaa acagccggaa atacttatca gagcaataaa taaaggagac
      781 ttttggctgt tttctgtgcg ggataatggt ataggaattg ataaagaata taatgataag
      841 atttttacta tttttcaaag attacataat aaatcaaaat acccgggaac gggaattggt
      901 ttatctattt gtaaaaaaat cgtggaaaga cacggaggcc gtatctgggt ggaatcaaaa
      961 cctggtcaag gctcgacttt ttatttcacc atacccagca agcaataaga taaggttttt
     1021 atggttaatg acaaacaaca aaaaatgatc gatattcttc ttattgaaga taatcccggt
     1081 gacgtaattc tcattaagga agctttcaaa gaagggcaaa catgcaataa actgaccgtt
     1141 gttggagatg gtgaagcagc tctttctttt ttgcgcaaag aaggtgcgta caaaaaatct
     1201 tcctgccctg atcttgtttt acttgatctt aacatgcccc gtaaagatgg ccgggaagta
     1261 ctcgccgaga ttaaaacaga tccggcacta aaacatattc cagttgtaat acttaccagc
     1321 tctgaagccc aacaagatat aatcaacgcc tacaatctgc aggcaaactg ttacataact
     1381 aaacctgttg atttaaaaca gttttttaat gtagtcaaac taatcgaaga tttttggtta
     1441 aaaacagtta aacttccata aaatatttca atgtttgata aacctattaa aatacttttg
     1501 ttcgaagaca atcttggcga tgcccggtta tttgcggaaa cgataaaaga gattccttct
     1561 cagcgttatg aaatcatcca tgtcaattcg cttgccgaag ggctcacggc gctcggcaat
     1621 aaaaccgccg atattattct gttggattta ggattacccg atagtcaaca tttggataca
     1681 ttaatcaagt taaaatctca agtcttggga gttcccattg ttattctgac cggcaatgac
     1741 gatgaagaaa tcgctttgcg agctgttcaa caaggcgctc aggactatct tgttaagcaa
     1801 aaaataaacg cagacgtact ttcgcgcgtt atatgctatt cgattgaaag acaaaaactg
     1861 caggatgaat tacgcagtct tgctttgatc gacgacttaa ccgggcttta taaccgccga
     1921 gggtttttta cccttgccga acattacctt aaacttgccg aacgtatgca caaaaatttc
     1981 tatttaattt tttccgacat tgatgacatg aaaaaaataa acgatgcttt tggccacctc
     2041 caaggtgacc tttttctaaa aaaaacggcc gaaatcttgc gttattcatt ccggaattcc
     2101 gatatcattg cccgtatagg cggagatgag tttgttgttt taattcccga aaccaccgat
     2161 tacaatgcaa aaagcgctat tacccgtctt caggaaaata taaaaaaata caataaaaaa
     2221 gaaaaaaaga atctttcttt gagcatgggc atagctaact ttcatcccaa tttgaaattt
     2281 tctttacagg atttactgga caaagccgat aagcatttat acgaagaaaa aaagaaaaaa
     2341 aacatgaaaa ataaaccagc aaataata
//
LOCUS       DASVIM010000053         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_447513, whole genome shotgun sequence.
ACCESSION   DASVIM010000053 DASVIM010000000
VERSION     DASVIM010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447513"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..949
                     /gene="prmC"
                     /locus_tag="VF305_01085"
     CDS             89..949
                     /gene="prmC"
                     /locus_tag="VF305_01085"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX7415759.1"
                     /translation="MTARKILHEATRDFEAAGIPSARLDAAVLLSFCLGRDRLDFLKN
                     PEMPISETQASLFKKTTARRLLWEPVAYITGRKEFWSFTLEVNNSVLIPRPDTEILVE
                     EALSICRKINSFEINILDMGTGSGAIAIALAKEMPHAKIVATDISSAALAVARKNAYN
                     LGLKNQIDFRPGNLFELVDDFFDIIVCNPPYISAQDYEKLPTGVKDYEPQDALLAGKK
                     GTEFYEKLIYQTDGYLKKNGWILLEIGATQEAVVRKIIEASGIFDSIGVRTDYAGLPR
                     VIRARRKISG"
     gene            942..2192
                     /gene="murA"
                     /locus_tag="VF305_01090"
     CDS             942..2192
                     /gene="murA"
                     /locus_tag="VF305_01090"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX7415760.1"
                     /translation="MDKIVIQGGKPLHGEVHISGAKNAALPILASALLTCGTNIFHNI
                     PDLLDIKTIKKLLKSLGAQIEGDETVRINVDKINSHTAPYDLVKTMRASVLVLGPLVA
                     RMGIARVSLPGGCAIGARPVNFHIKALQDLGAHVELNNGYIEAKAKKLKGADIYFDIP
                     TVTGTENIMMAATLAEGTTILNNAACEPEVVNLADVLKGMGAKISGAGTDVITIEGVT
                     SLKPTEASIIPDRIEAGTFMIAAGMTHGEVNIKGCNPHHLEALINKLRDTGMKISPIE
                     DGLQVSARKKINSVDIKTLPHPGFPTDLQAQMMAYMSIGSGLSVISETVFENRFMHVS
                     ELTRMGADIVIQGGSAIVRGVPHLRGAQTMATDLRASASLILAALVAQGTTEISRVYH
                     IDRGYQEIEKKFFALGANIKRTAQ"
     gene            2194..>2996
                     /gene="hisD"
                     /locus_tag="VF305_01095"
     CDS             2194..>2996
                     /gene="hisD"
                     /locus_tag="VF305_01095"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX7415761.1"
                     /translation="MKIIKANAIEFAEFFRKLRQRGGSFTPELLSSVAEIVSDVAAKG
                     DEALFHYTAKFDHHELNAKTVEVTPAQRKEALAQVKPEDWEVIKLAAERIEKYHRNQI
                     VQGWSVADEEGIELGQRILPLRRVGVYAPGGKAFYPSTLLMAAIPARLAGVEEIILVS
                     PVKDGKLNPLIAAAAEVSGVQRIFKVGGAQAIAALAYGTKSIPQVDKIVGPGNAYVAA
                     AKKLVFGQVDIDMIAGPSEIVVIADKTAPASFVAADMLAQAEHDEMAAAV"
BASE COUNT          836 a          650 c          759 g          751 t
ORIGIN      
        1 ttggcgacac attaccaggc agaagcgtta aataaaccag gaaattaata tccatctgag
       61 acattagctc tctaagcgga tcagaagtat gactgctcgg aaaattctcc atgaggcaac
      121 ccgggatttt gaagctgcgg gaattccttc ggcgcgattg gatgccgcgg tattactttc
      181 tttttgctta ggacgagacc ggctcgattt tctcaaaaat ccggaaatgc ccattagcga
      241 gactcaagct tctttattta aaaaaaccac cgccagaaga ttgctatggg aaccggtggc
      301 ctatattacc ggacgcaaag aattttggtc ttttacgctg gaagtaaata actctgtatt
      361 gattccccgg ccggatacag aaatccttgt tgaagaagcc ctcagcattt gccgcaagat
      421 taattctttc gaaataaata ttctagacat gggcacgggc agcggtgcga ttgctatagc
      481 tttagccaaa gaaatgcctc acgccaaaat tgtggccaca gacatttcct ctgccgccct
      541 ggccgtagcc aggaagaacg cctataattt ggggctgaaa aatcaaattg attttcgccc
      601 aggcaattta tttgagttgg ttgacgattt ttttgatata atcgtttgca acccacccta
      661 catttcggcc caagattatg aaaaacttcc taccggggtg aaagattatg agccgcaaga
      721 tgccctttta gccggtaaaa agggaactga attttacgaa aaattgattt atcagacgga
      781 cggctatttg aaaaaaaatg gctggatttt gttggagata ggagctactc aggaagcagt
      841 cgtgaggaag attattgagg cttccggaat tttcgatagc atcggcgttc ggactgatta
      901 tgccggtctg ccgcgtgtaa ttcgagcaag gagaaaaatt agtggataaa atagttattc
      961 aaggcggcaa acctctgcat ggtgaagttc atattagcgg ggcaaaaaat gccgcgctac
     1021 cgattttggc ctcggcgctt ttaacctgcg gaaccaatat attccataat attcccgatc
     1081 tcctggatat caaaacaata aaaaaacttt tgaagagcct cggcgctcaa atcgaaggcg
     1141 acgaaactgt ccggatcaac gttgataaaa ttaacagcca cacggctcct tatgatttag
     1201 tcaagacgat gcgggcttcc gttttggttt tggggccgct ggttgcccgc atgggcatcg
     1261 cgcgtgtttc tcttcccgga ggctgcgcca tcggcgcccg gccggtaaat ttccatatta
     1321 aagccctgca ggatctcggc gctcacgtcg aattgaataa cggctacatt gaagcgaagg
     1381 ctaaaaaatt gaagggtgcg gatatttatt ttgacatacc cacagtaacc ggcaccgaaa
     1441 atataatgat ggcggcaact ttagctgaag gcactactat tcttaacaac gcggcctgtg
     1501 agccggaggt tgtcaatctg gcggacgttt taaagggcat gggagcaaaa atcagcggag
     1561 cgggtacgga tgtaataaca attgaaggtg ttacctcgct aaaacctacg gaagcttcaa
     1621 ttattcccga ccggattgag gcgggaactt ttatgattgc cgccggtatg acccacggag
     1681 aggtaaacat aaaaggttgc aatccccacc acctggaagc cttgatcaac aaactgcggg
     1741 atacgggcat gaagatttcg cccattgaag acggcctgca agtatctgcg aggaaaaaaa
     1801 tcaatagtgt cgatatcaaa actcttcctc atccgggatt ccctaccgat ttacaggcgc
     1861 agatgatggc ttatatgtcc atcggcagcg gtttgagcgt gatttccgaa actgtttttg
     1921 aaaaccgttt tatgcatgtc agcgaactca cgcggatggg agccgatatc gttattcagg
     1981 gtggtagcgc gattgtccgg ggagtgcctc acttacgcgg cgcacaaacg atggctacgg
     2041 atttgcgggc ttcggcttct ctcattttag ccgctctggt tgcacaagga acaacggaga
     2101 tttcccgcgt ttatcatatt gaccgcggtt atcaggaaat agagaagaaa tttttcgcac
     2161 tgggtgcaaa tataaaaagg acggcacaat aatatgaaaa taatcaaagc taacgctatt
     2221 gagtttgcag aatttttccg taaattgcgc caaagaggcg gctcttttac cccggagctt
     2281 ttatcatctg tagcggaaat agtgagcgat gtcgccgcca aaggcgatga agctctattt
     2341 cattatacag ccaaatttga ccaccatgaa ttaaacgcga aaacagtaga agtgacacct
     2401 gctcaaagaa aagaagcgct ggcacaagta aagccggaag attgggaagt tataaagttg
     2461 gctgccgagc gtatcgaaaa atatcaccgc aatcaaattg ttcaaggatg gtctgttgcc
     2521 gatgaggaag gcatagaatt gggtcaacga attttgcctt tgcggcgcgt gggggtttat
     2581 gctccgggag gcaaagcttt ttatccttcg acattgctta tggcggcaat tccggcgcgc
     2641 ctggccggag tggaagaaat tattttggtc agtcccgtaa aagacggcaa attaaatccg
     2701 ttaattgccg cagcggcaga agttagcggc gtacagcgca tatttaaggt tggcggcgcg
     2761 caggccatag cagcgctggc ttacggcaca aaatctattc cacaagttga taaaattgtg
     2821 ggaccgggaa acgcttacgt tgctgcagcg aagaaactgg tttttggcca ggtggatata
     2881 gatatgattg ccggtcccag cgagattgtc gttattgccg ataaaacggc accagcttct
     2941 tttgtggcgg cggatatgct ggcacaagcc gaacacgatg aaatggctgc ggctgt
//
LOCUS       DASVIM010000054         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_874201, whole genome shotgun sequence.
ACCESSION   DASVIM010000054 DASVIM010000000
VERSION     DASVIM010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874201"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VF305_01100"
     CDS             complement(<1..211)
                     /locus_tag="VF305_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415762.1"
                     /translation="MILARINKSLRESLIITTVTLVIAILFNLLRPAGIPLFDFSSVN
                     LINEQQAKIPTITLTEAYDLYQKNKV"
     gene            complement(259..768)
                     /locus_tag="VF305_01105"
     CDS             complement(259..768)
                     /locus_tag="VF305_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7415763.1"
                     /translation="MAENEIKQIMINGHLVGIVGLDDIIQKIAVGHQGKSDKDIINSL
                     LSEISVNNYVPPGAVESYGKALLREFKIAQGLLVAEEPSPELDIAVLGMGCARCNQLE
                     DDVRGLLSEMKIAASLRHITDFKEIARFGVISTPALAINNKVVSVGEVPPKSKIRQWI
                     IDAYPQPLK"
     gene            1066..>1813
                     /locus_tag="VF305_01110"
     CDS             1066..>1813
                     /locus_tag="VF305_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX7415764.1"
                     /translation="MDIINRAKSQGRTTLTEAEAKEVLKKYGVPVVEEKSVKSIEEAE
                     IAAEKIGYPVVLKGLGARLAHKTERGLVKLNLKNKEDIRTAALYIKDAAGSDLEGFLI
                     QPMLEGKREFVAGLFHDDQFGPTVMFGLGGIFTEAIEDVVFALAPLEKNEAQRMIEQL
                     EAQKLLGEFRGEKAPDRDALINVLVGLSKIGKEIPEIREIDINPLLVTADGKVTAVDA
                     LIVLGEAKEQKISQLPIEPRAIWNLFYPKSI"
BASE COUNT          572 a          304 c          412 g          525 t
ORIGIN      
        1 tgaccttgtt tttttgatac agatcgtatg cttcggtaag agttattgtg gggattttcg
       61 cttgttgttc attaattaaa tttaccgagg aaaagtcgaa taaaggaatg cccgcaggcc
      121 gaagcaaatt gaacaaaatg gcgataacta aagtaacggt tgtgatgatt aagctttccc
      181 gaagggactt attaattctt gctaaaatca tatttgtaaa caatccttca ggagttgtca
      241 tgagcagtat atttatcttt attttaacgg ttgagggtag gcatcaatta tccattgacg
      301 gattttactt ttcggaggta cttcacccac cgatacaacc ttattattta ttgccaaggc
      361 cggggtgctt ataactccaa aacgcgctat ttccttaaag tcggtaatat ggcgcaagct
      421 agcggcaatt ttcatttccg aaagtaaacc acggacatca tcctccaact gattacagcg
      481 tgcgcagccc atacccagaa cggctatatc caattcggga ctgggctcct cagcgacgag
      541 cagcccctgc gcaatcttaa attcgcgtaa tagcgccttt ccgtatgatt ccactgcacc
      601 gggaggcaca taattattga cagagatctc gcttagcaaa ctattaatga tgtccttgtc
      661 acttttgccc tgatggccta cggctatttt ttgtattata tcgtctaaac cgacaatccc
      721 gaccaagtgt ccgttaatca taatttgttt aatttcattt tcagccatag attctatttg
      781 atagaggtgt aaaaatatcc ttttaattat tttagcgaaa gcataatatt tttgtgtata
      841 aatgtattgg tacaaaagag tttcgtcaag aagaaaaatt tgttttataa atacggaaat
      901 aaaatttaca aggaaagaag atgattgcat tatcggacgg catgtgttag tgactaggag
      961 tcagggcggc aagttgtata taatgtaagt atttaatata attaatgtaa ttcaaaagta
     1021 tcattacaag ggaatctgtt cgcctgtcta tttggagggg aaaaattgga tataattaat
     1081 agagcaaagt cacagggaag aacgacgctg acggaagcgg aagctaaaga ggtgttaaaa
     1141 aaatacggcg ttccggtagt ggaagaaaaa tcagttaagt ctatagaaga agcggagatc
     1201 gcggcggaaa aaattggtta tcccgttgtt ttaaaaggtt taggcgctcg tttggctcac
     1261 aagacggaaa gaggtttggt taaactgaac ttaaaaaaca aggaagatat cagaactgct
     1321 gcgctatata taaaggatgc agccggaagc gatttagaag gctttttaat ccagccgatg
     1381 ttggagggta aaagagaatt tgtagcaggg cttttccatg atgatcagtt tggtcctacg
     1441 gtaatgttcg gattgggcgg aatttttacg gaagcaatag aggatgttgt gtttgctctt
     1501 gcccctttgg aaaaaaatga agcgcaaaga atgattgagc aattggaggc gcaaaaactt
     1561 ttgggagaat ttcgcggaga aaaagcgccg gatagagacg cgctgattaa tgttttagtc
     1621 ggtttatcaa agataggaaa agaaataccc gaaataagag agattgatat taatccatta
     1681 ttagtgactg ccgacggaaa ggttacggct gttgatgccc ttatcgtctt aggggaagca
     1741 aaagaacaaa aaatatctca attgcctata gagccgagag caatctggaa tttattctat
     1801 ccgaaatcca ttg
//
LOCUS       DASVIM010000055         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_789538, whole genome shotgun sequence.
ACCESSION   DASVIM010000055 DASVIM010000000
VERSION     DASVIM010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789538"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1057
                     /locus_tag="VF305_01115"
     CDS             251..1057
                     /locus_tag="VF305_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX7415765.1"
                     /translation="MKAGFIQFSPEFGRVDANIDKALSMMEKADAQLIVLPELFNTGY
                     LFISQKEAFEMAEEIPTGKTTKALSSIARKKKVYIVAGIAESVGENLYNSAVLISPKG
                     YVATYRKIHLFNEEKLWFKPGNKSFAVYDIGQCKIGIMICFDWFFPESARILALRGAD
                     IICHPSNLVLPHCQDAMVTRCLENNIYVITANRTGREERSGKSLSYTGKSQITSPDAR
                     ILYRADAQNDEIGIVEIDVQMARDKKINNYNDIFADRRVEYYQDLVKANF"
     gene            1155..1229
                     /locus_tag="VF305_01120"
     tRNA            1155..1229
                     /locus_tag="VF305_01120"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1187..1189,aa:Val,seq:cac)
     gene            complement(1296..1538)
                     /locus_tag="VF305_01125"
     CDS             complement(1296..1538)
                     /locus_tag="VF305_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415766.1"
                     /translation="MTDSYSSGWQVKPLIGSLQQPYIVMPVNYALMAIPLSASERHFR
                     VADEPISFVMVKWIALSAIIIYLTLILIAVRKPTML"
BASE COUNT          618 a          366 c          408 g          587 t
ORIGIN      
        1 aaacgttgta ttgtcaccat gtgtatcggt ggtggaatgg gagctgccgg tatatacgaa
       61 atgctttaat tggaatatag tcccttctag cgccaagcta taacttcgct caatccctcc
      121 gtcatccttt tgttgcggga tgacggaggg attggcggcg aaaagtcttt ccattgtttt
      181 gtcacatcta attaaatcat cttgacagaa atgttattcg aaatgtagcc ttaatgctaa
      241 gagggaaaag atgaaagcag gttttattca gttttcgccg gaattcggca gggtcgatgc
      301 aaacattgat aaagcactat caatgatgga aaaggccgat gcccagctga ttgttttgcc
      361 ggaactattt aataccgggt atctttttat ttcgcaaaaa gaagccttcg aaatggccga
      421 agaaattccc acgggaaaaa caacaaaagc tttatcttcg atagcccgga agaaaaaagt
      481 ttatattgta gccggaattg ccgagagcgt aggtgaaaat ttatataact ccgccgtttt
      541 gatttcacct aaaggttatg tagccactta ccgtaaaatt catcttttta atgaagaaaa
      601 actgtggttt aagccgggta ataaatcttt tgcggtttat gatatcggtc aatgtaagat
      661 cggaatcatg atatgcttcg actggttttt cccggaatcc gcgagaattt tagctctaag
      721 aggtgcggat attatatgcc atccctccaa tcttgttctt ccccactgtc aggatgccat
      781 ggttacccgg tgcttggaaa ataatattta tgttataacc gccaatcgca ccggtcgtga
      841 agaaagaagc ggcaaaagtt tatcttatac cggcaaaagc cagataacgt ctcccgatgc
      901 acgcatcttg tatcgtgccg atgcccaaaa tgatgaaatc ggcatcgtag aaattgatgt
      961 gcaaatggcc agggacaaaa aaataaataa ctataatgat atttttgccg atcgcagggt
     1021 agagtattat caggatttgg ttaaagctaa tttctaatgt cttcgataat aaccaaagca
     1081 gttttttaat tgcttaacga agaaactttt ttccttgaaa tgagagatgc tttgagctat
     1141 agcagacggc acttgggcgg ttaactcagc gggagagtgc taccttcaca cggtagaagt
     1201 cactggttca atcccagtac cgcctaccat gatattcaag ggattagcga ttaatcgcta
     1261 atcctttttt tgtttagaaa tatggtcatg taattttaca acattgtcgg ttttcgtacc
     1321 gcgattaaaa taagagttaa ataaataatg atagcagata gagctatcca tttcaccatg
     1381 acaaaagata ttggttcgtc cgcaacacga aagtgacgtt cacttgcaga caacgggatt
     1441 gccataagcg cataattaac gggcatgaca atataaggtt gctgtaagct gccgataaga
     1501 ggttttacct gccagccgct actatacgaa tctgtcaagt taagtccatc cttaaacaat
     1561 taagggaatg atgattagaa taatggtggc gcaacaatgc tgaaatttta ttccggtttt
     1621 atcggcggta atttttctac ataaccattc gaaaaagttt tgaatcatta attgataacc
     1681 tttttagctg cggcgtaaat taaaagccaa ttcactatag ataagctggt caatctaaat
     1741 ttgtttttcc ccaatgcatg acgcaatctt gttttagtga aatgattgat atggccgcac
     1801 ctgaatttat gataacgact gccttttata aaacccatat ataagcagtt aaaattaaat
     1861 agtataaaaa agggacccat gaaaaaatcg tatggaactg taattaaaaa tattccatca
     1921 tccctgagaa ttctatggac ttcagccaga acgtattcga aatttagtag gtgttccag
//
LOCUS       DASVIM010000056         5703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_533061, whole genome shotgun sequence.
ACCESSION   DASVIM010000056 DASVIM010000000
VERSION     DASVIM010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533061"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="VF305_01130"
     CDS             complement(<1..1425)
                     /locus_tag="VF305_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415767.1"
                     /translation="MIITNIFNFIRNLIRFFGACRIFIGKNPASVTTPAIILFPIMPS
                     QLNCGFAGLMTFRHKQKSTDLTADLALTRLWEKINGSGLKNVLAGKIAAANYWSGLET
                     LEFMEKAILELKEEITQEILFFQPVRAKKLFGLAEKMKLFLAQEEKLLENNAEKFTSA
                     DLEIINSRIILLKDIQWILENDILANFEKITDLTGAGKITANIPAAFRKYRKINFLLN
                     ALDRLEVRGRDSAGLQIVFTLEKEKTMESIMLDLKERSLYEDYLMRSKAGDLVNGSIR
                     IAFPVFAAAKERISLTFTYKTFSIVGELGRNVRDLRQTIGKDQILQYFAKLNTVCETA
                     LTHTRWASVGSITEENCHPVNNYKPNQASQSFPFYPKSKAQINIVLNGDIDNYLALRA
                     ELEENKELIAPEVTTDTKIIPLQIEKYLTAGHDLSESFRLAVNDFEGSHAIVMTSDLE
                     PGKIFLALKGSGQCIYVGISSDQYM"
     gene            complement(1425..2630)
                     /locus_tag="VF305_01135"
     CDS             complement(1425..2630)
                     /locus_tag="VF305_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HEX7415768.1"
                     /translation="MTNPFSVEIGDEVNTERIDSSLIIYGATRIYGENTLIAPDVKLG
                     YEGPVTLIDCQLGAAVELRGGYFAGSVFLDKAKIGSNAQVRSGCLLEEEAGANHTVGL
                     KQTILFPFVTLGSLINFCDCLMAGGTSRKNHSEVGSSYIHFNYTPQQDKATSSLIGSV
                     PRGVMLREPPIFLGGQGGIVGPVQIDYGTVIPAGIICRQDCMNEGMRWSSSASPNRKK
                     EFVAGVYGDIIRKVNNNIAYVANLLALKQWYSCVRREFFIREKYGNALYSGACNVLTE
                     AVAERIKQVRLFAQNLENSIALGPKKMKRGLSDNVWRTQKLFLQNWPQMEAYLACNNE
                     EKIGLRKRESFVKTIQRQADKGNSYLETIQSLTPAQTREGTDWLNDIVVGVTTGCLKK
                     FRASRQEDK"
     gene            complement(2720..3025)
                     /locus_tag="VF305_01140"
     CDS             complement(2720..3025)
                     /locus_tag="VF305_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7415769.1"
                     /translation="MRSKKKNKSAVVMDVRETRYAAVEEIMEKKLEDLATVLSASSDR
                     KSKLYEEVMAMVEKGLFKIALRRSNYVKSAAAVFLGINRNTFTDKMAKLGINCEKIK"
     gene            complement(3108..3500)
                     /locus_tag="VF305_01145"
     CDS             complement(3108..3500)
                     /locus_tag="VF305_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415770.1"
                     /translation="MAILFVSSLAVGVEASLREIIVTEDGLKIKRFFREKEFVWTEIT
                     QLAVVVLRNKAYFLLTTTKGFYIFSNLMEKHVLLIRFLLDRLGEEKVEVEIKNYLNNP
                     VERISLIVMSWAALIVIIAIIVIKLMPA"
     gene            complement(3637..4122)
                     /gene="moaC"
                     /locus_tag="VF305_01150"
     CDS             complement(3637..4122)
                     /gene="moaC"
                     /locus_tag="VF305_01150"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX7415771.1"
                     /translation="MKKLSHINDEGQVQMVDVTGKNKTIRVAKAGGKVKMDPQTLKMI
                     ENGHIAKGNVLTTAKIAGIMAAKKTGELIPLCHPLDLTGIDINLKIDHKNSQIIIEAQ
                     VQTVGKTGVEMEALTAVSVAGLTIYDMCKSADKKIIIGEIMLLEKSGGKSGTFTRNVT
                     K"
     gene            4298..>5703
                     /locus_tag="VF305_01155"
     CDS             4298..>5703
                     /locus_tag="VF305_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX7415772.1"
                     /translation="MFVKVFSMSVIGMESYPVFVEIDISQGLPQFATVGLPDTSVKES
                     KDRIKAAIKNSGYRFPRSHVTVNLAPADIKKEGTGFDLPIAVGILAAEELIKETALHD
                     CFFIGELSLDGSIKRVSGVLTAVFKAKEIGIKSVFVPEENAAEAAMVEGINVFPVKTL
                     PDVVEFLNGRSNIEPLSLDVSKIFQKTRQYEVDFNEIKGQKQALRALEIAAAGGHNAL
                     MIGPPGSGKSMLAKRLPTILPDISFEEAIEVTKVFSVAGSLNKNGSLVAVRPFRSPHH
                     TISDVGLVGGGQMPRPGEISLAHLGVLFLDELPEFKKNALEALRQPMEEGAITITRST
                     ITASFPAKFMLVAAMNPCPCGYFGDRIRTCRCSAQQIRQYQGKISGPLLDRIDLHIEV
                     PSVKYRALSDKELGESSSTIKDRVNYARELQKKRFGTKNILCNARMTEKQIKNFCPIN
                     EDSHKLIEMAIEKLGLSAR"
BASE COUNT         1595 a         1280 c         1102 g         1726 t
ORIGIN      
        1 catgtactga tcggagctta tcccgacata aatgcactga ccgctgcctt tgagcgccaa
       61 aaatatttta cccggttcca gatcactggt cataactatg gcatgggatc cctcaaaatc
      121 attcacggca agacggaagg attcggacaa atcatggccc gccgttagat atttttcaat
      181 ttgcagggga atgattttag tatcagttgt aacttccggt gcaatcagtt ccttattttc
      241 ctccaactcg gctcgcaacg ccaaataatt gtcaatatcg ccgtttaaga caatattaat
      301 ttgagctttg gattttggat aaaagggaaa ggattgactg gcctgattag gcttatagtt
      361 attgacagga tgacaatttt cttccgtgat agaaccaacc gaagcccagc gagtatgggt
      421 aagagccgtc tcgcatacag tattgagctt ggcaaaatat tggagaattt ggtcttttcc
      481 aattgtttgt cttaaatctc gcacattgcg cccaagttca ccgacaatgg aaaatgtttt
      541 ataggtaaaa gttaagctta ttctttcttt tgctgccgca aatacaggga aagcaatccg
      601 aatagaaccg ttgacaaggt ctcccgcctt cgacctcatt aaataatcct cgtataagct
      661 tctttccttc aagtcaagca ttatgctttc cattgttttt tctttttcca gggtaaagac
      721 aatttgcagt cccgcagaat ctctgcccct cacttccagg cggtccagtg cattgagcag
      781 gaaattaatt ttccggtatt ttctaaaagc cgcagggata tttgcagtta ttttccccgc
      841 tcccgtgaga tcagtaattt tctcgaaatt agcgaggatg tcattttcta aaatccattg
      901 gatatctttg agcaggataa tccggctatt gataatttcc aaatcagcgg aagtaaactt
      961 ttcggcgtta ttttcgagca gcttctcctc ttgggctaaa aataatttca tcttttcagc
     1021 taaaccaaag agctttttgg ctcttaccgg ttgaaagaaa agaatttcct gcgtaatttc
     1081 ttccttcagt tccagtattg ctttttccat gaactcgagc gtttccaatc cgctccagta
     1141 atttgccgcg gcaatcttcc cagctaaaac attctttaaa cccgacccat tgattttttc
     1201 ccacaacctt gtaagagcaa gatcggcagt taaatcagtt gatttttgct tatgccggaa
     1261 agtcattaaa ccggcaaagc cgcagttaag ctgagaaggc ataatcggga aaagaattat
     1321 cgccggagtt gtaacactcg ccggattctt tccgataaat attcggcagg ctccaaaaaa
     1381 ccggatcaag tttcttataa agttgaaaat atttgtaata atcattattt gtcttcctgc
     1441 cgtgaagcgc gaaacttctt tagacacccg gtagtaaccc ccacaacaat atcgtttaac
     1501 caatctgtac cttcccgggt ttgtgcagga gttaaagact gtattgtctc cagataagaa
     1561 ttacctttgt ctgcctggcg ctgtatcgtt tttacaaacg attctctttt cctcagacct
     1621 attttttctt cgttgttgca ggcaagatat gcctccatct gcggccaatt ttgcaaaaaa
     1681 agtttttgag tacgccagac attatccgat aagcctcgct tcattttttt tggacctaag
     1741 gcaatggaat tctctaagtt ctgcgcaaac aaacgtacct gcttgattct ttcagcaacg
     1801 gcttcagtta aaacattgca ggcgcctgaa taaagagcat tgccatactt ctctcttata
     1861 aagaactctc gacgcacgca gctataccat tgctttaacg ccaataaatt tgccacataa
     1921 gctatattat tattaacttt gcggataata tcgccataaa caccggcaac aaactctttc
     1981 ttccggttag gtgaagctga ggaggaccaa cgcattcctt cgttcataca atcctgccgg
     2041 caaataatgc ctgcgggaat cactgtacca taatctattt gcaccggccc tacaatgcca
     2101 ccttggccgc ccaggaatat tggtggttcg cgcaacatta ctccccgcgg cacgctgccg
     2161 attagtgaag aagttgcttt atcctgctgg ggagtataat taaaatgaat ataagagctg
     2221 cctacttcgc tatgattctt ccggctggtt ccacccgcca tcaaacaatc gcaaaaatta
     2281 atgagactgc ccagagtaac aaaagggaaa agtattgttt gctttaagcc aacggtgtgg
     2341 tttgcgccag cttcttcttc caaaagacaa ccggagcgga cttgcgcatt gctgcctatc
     2401 tttgccttat ccaagaaaac cgagccggca aaatatccac ccctaagttc caccgcagcg
     2461 cccaactgac agtcaattaa agtaacaggc ccctcgtatc ccagttttac atctggagct
     2521 atcagcgtat tttcgccgta aattcttgtt gcaccataaa ttattaaaga actgtcaatc
     2581 ctctctgtat ttacctcgtc acctatttcc acagaaaaag ggttcgtcat ccgaacccct
     2641 ttctttaaga gacatgttac tttttccggt aatatctcag tttcttttat ggctctagtc
     2701 ataaattttt ttatagtagc tacttgattt tttcgcagtt gataccaagt ttggccatct
     2761 tatcggtaaa tgtattgcgg ttaataccca aaaatactgc tgccgcgctt tttacgtagt
     2821 tagacctgcg cagggctatt ttaaataaac ccttttccac catggccatt acttcttcat
     2881 aaagcttgct tttgcgatca ctggaagccg ataaaacagt agcaaggtct tccagctttt
     2941 tttccattat ttcttcaaca gctgcgtaac gggtttccct gacatccatc actactgctg
     3001 atttgttttt ctttttcgac cgcattcttg tgaagctcct ttaattttag ccaatattta
     3061 ctacgacacc aaaaagttta tatttttgaa acacgttaaa ttcgaattta agccggcatt
     3121 aatttgataa ctataatggc aataatgact atcaacgcag cccaactcat tacaatcagc
     3181 gaaatccgtt cgacgggatt attcaaatag tttttaattt ctacttccac tttttcttca
     3241 cccaacctat cgagaagaaa ccggataagt aagacatgtt tttccataag attggaaaag
     3301 atataaaatc ctttagttgt tgttaataaa aagtatgctt tgttccgcag gacaacaact
     3361 gccaactggg taatttccgt ccagacaaat tctttctccc gaaaaaacct ttttattttt
     3421 agcccgtctt cagtaacaat gatttcccta agtgacgctt caacacccac ggctaaggaa
     3481 ctgacaaata aaatcgccaa gattattttt tcccacgttt gacctgtaaa taaactaagt
     3541 aaaaaaagca aaaataaaag cacaataatc gcaatataag gaatgagaaa ggatttttta
     3601 attttatagg tattttgcac ttttcccttt ctccgattat tttgttacgt ttctcgtaaa
     3661 cgtgccgctt ttaccgccgc ttttttccag aagcatgatt tccccgataa ttattttctt
     3721 atcggccgat ttgcacatat cgtaaatagt taaacctgcg acgctcactg cggtaagcgc
     3781 ttccatttcc acgccagttt tgccgaccgt ttgtacttga gcctctatta taatttgcga
     3841 atttttatga tcaattttta aatttatgtc aattcccgtt aaatccagcg gatgacaaag
     3901 gggaataagc tcgcctgttt tcttggcggc cataatgccc gcaatttttg ctgtggtcaa
     3961 aacattgccc ttggcaatat ggccattttc aatcatcttc agcgtttggg ggtccatctt
     4021 aactttgccc ccagctttgg ccacacgaat tgtcttgttc ttgcctgtaa catccaccat
     4081 ttggacttgg ccttcatcat tgatgtggga taactttttc atggcagcta tggacttaat
     4141 taagaattaa attattggaa ctaatgattt cgcgcgactc taacataggg caattaatga
     4201 gtcaagaaag tctaacattt tttattgaat taaaaaactt gatataaata tcgcgattcg
     4261 ctagtatttt tgacaaaatt gaagctggag aatattagtg ttcgttaaag tatttagcat
     4321 gagcgttatt ggcatggaat cataccctgt ttttgtggaa atagacatat cccagggact
     4381 gcctcaattt gccactgttg gactgcccga tacctcggtc aaagaaagca aggatcgaat
     4441 taaagccgcc ataaagaatt ccggttatcg ctttccgcgc agtcatgtaa ccgtaaattt
     4501 agctcccgct gacattaaaa aagaaggaac cgggtttgat ctccctatcg ccgtaggcat
     4561 tttagccgcg gaagaattga ttaaggaaac tgctttgcat gattgttttt tcatcggaga
     4621 attgtcgctc gatggcagta ttaaacgagt aagcggtgtg ctaaccgctg tctttaaagc
     4681 aaaagaaata ggcatcaagt cagttttcgt tcccgaagaa aatgctgcgg aagccgcgat
     4741 ggtggaagga attaatgttt tcccggtaaa aactttgcct gatgtagtag aatttttaaa
     4801 tggcagaagt aatattgaac ctctttccct ggacgtaagt aaaatttttc agaaaacacg
     4861 tcaatatgaa gtcgatttta atgaaattaa aggacagaag caagccctga gagcgctgga
     4921 aattgcggcg gccggaggcc acaatgccct gatgatcggt cccccgggct ccggcaaaag
     4981 tatgctggca aaaagactgc ccacgatttt gcccgacatt tctttcgagg aggcgattga
     5041 agtgacaaaa gtattttccg tcgccggttc attgaataaa aacggatctc tagttgccgt
     5101 gcgtcccttt cgttcgccgc atcacacaat atccgatgtc ggtctggttg gaggcggtca
     5161 gatgcctcga ccgggtgaaa tcagcctggc ccatctagga gttctttttt tggatgaact
     5221 acccgagttc aaaaaaaacg cactggaagc tctccgtcaa ccaatggagg aaggcgctat
     5281 aacaattact cgctctacga taacagcatc tttccctgca aaatttatgc tggttgccgc
     5341 gatgaatccc tgtccctgcg gttattttgg cgatcgtata cgcacctgcc gctgcagtgc
     5401 gcagcaaatt cggcagtatc agggaaaaat ttccggtccg ttattggatc gaatagattt
     5461 gcatatcgaa gtgccttcgg tcaaatatcg ggccctttcc gacaaagaat tgggggaatc
     5521 ctcttcaaca attaaagatc gcgtaaatta tgcccgggaa ttgcaaaaaa agcgttttgg
     5581 tactaaaaat attctctgta atgcccgcat gacggaaaag caaattaaga atttttgccc
     5641 cataaatgaa gattcgcata agctcatcga gatggccata gaaaaactgg gattaagcgc
     5701 ccg
//
LOCUS       DASVIM010000057         1741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_703803, whole genome shotgun sequence.
ACCESSION   DASVIM010000057 DASVIM010000000
VERSION     DASVIM010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703803"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VF305_01160"
     CDS             complement(<1..477)
                     /locus_tag="VF305_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEX7415773.1"
                     /translation="MLQKIFFIAVAAFLLLFAGHAYCQTSFAILPMEVRGDVKPEKVE
                     GAMSNLYQVLVNSKKYRIVDREHVNSVLSEQSFQISGVTNNKDTVKLGKVLNVDKLVV
                     TSIYMKSADQFAINLRVIDVTTGQAEFYKEISYLNYTPADHGRFCATEVIAHYPLLG"
     gene            complement(553..>1741)
                     /locus_tag="VF305_01165"
     CDS             complement(553..>1741)
                     /locus_tag="VF305_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX7415774.1"
                     /translation="EISGVYDEIKYALENVRSWAKTKKVETPVFLLPSKSYVQKEPYG
                     VTLIIAPWNYPFQLLMAPLIGAIAAGNTAILKPSEVSANTAAVMEKIINKAFPKEYLH
                     VVQGGAVETQALLDLPVDYIFYTGSAAVGKIVMSAAAKNLTPLTLELGGKSPAIVHED
                     ANLTHAARKIAWGKFINAGQTCIAPDYVLIHTNRKDEFLEELKKAIRDFYGENAAAHP
                     RYCRIINDRHFQRLSALLDTKKIVYGGNAHPADRYIEPTILYPVEWNEPVMRDEIFGP
                     VLPLLLYSSLDDIIRKINERPKPLALYLFSDDQSVQKKITAEISYGGGAINNTIFHFV
                     SHFLPFGGVGASGMGSYHGKASFNTFTHFKSILQSSSRIDPLAVTYHNKTFFMNIMRK
                     IMG"
BASE COUNT          468 a          383 c          378 g          512 t
ORIGIN      
        1 tcccaacaga ggataatggg caataacctc tgtggcgcaa aaacggccgt ggtcggccgg
       61 cgtataattc agatatgata tttctttgta aaattcggcc tgcccggtgg taacatcaat
      121 tactcttaaa ttgatggcaa attgatcggc gcttttcata taaatgctgg tgactactaa
      181 tttgtcaacg ttcagcactt tacccaactt aactgtatct ttattgttcg tgacgccgga
      241 gatctggaaa gactgttcgc taagcacaga attgacgtgc tcacgatcaa cgatcctata
      301 ctttttagaa ttaaccagga cctgatacaa attgctcatt gcgccttcaa ctttttccgg
      361 cttaacgtct ccccgcactt ccataggcag aatagcaaat gatgtttggc aataggcatg
      421 tccggcaaat aaaagcagaa aagctgcaac tgcaataaaa aatatttttt gaagcattga
      481 ttgcacctcc gaatttgata gatgatttaa tggacatatc tgatgtgctt ttaccattta
      541 atgactttgt tctcaaccca ttatcttccg cataatattc atgaaaaacg ttttgttgtg
      601 atacgttacg gctaagggat caattctgct ggagctttga agtatgcttt taaaatgagt
      661 aaatgtgttg aaactggctt ttccgtggta ggagcccatc ccgcttgcac ctactcctcc
      721 aaagggcaga aaatgggata caaaatgaaa gatagtattg ttaatagcgc cgcccccgta
      781 agaaatttct gcggtgattt ttttttgtac cgattggtca tcgctgaaca gataaagcgc
      841 caaaggtttc ggccgttcat taatttttcg gattatatca tcaaggctgg aatataaaag
      901 tagcggcaaa acaggtccga agatttcatc gcgcataacc ggttcattcc attccacagg
      961 ataaagaatt gtgggctcaa tataacggtc agccggatga gcatttccgc cgtagacaat
     1021 ttttttggtg tcgagcaagg cgctcaggcg ctgaaaatgc cggtcgttga ttatccggca
     1081 ataccgtgga tgagccgccg cgttttcacc gtaaaaatca cgtatcgctt tttttaattc
     1141 ttccaggaat tcatcttttc ggttggtatg aatcagaaca taatccggag ctatacaggt
     1201 ttgtccggca ttgatgaatt tcccccaggc tattttacgc gccgcgtggg tcagattggc
     1261 gtcttcatgc acaatcgccg ggcttttgcc gcccaactcc agtgtgaggg gagtcaaatt
     1321 tttggctgcc gcgctcatca cgattttacc tacggcagcg ctgccggtat agaatatgta
     1381 atctacaggc aaatccaaaa gcgcctgcgt ttccactgcg ccgccctgta ctacatgcaa
     1441 atattcttta gggaaagctt tattaatgat tttttccatt actgccgccg tatttgccga
     1501 aacttccgat ggtttaagaa ttgccgtgtt tccggccgca atcgcgccga taagcggtgc
     1561 cataagaagc tgaaacggat aattccaggg agctattata agagttacac cgtaaggttc
     1621 tttttgcacg taacttttag agggcaaaag aaaaaccgga gtttcgactt ttttggtctt
     1681 agcccatgaa cgaacatttt ccagggcata tttgatttcg tcatacacgc cggaaatctc
     1741 t
//
LOCUS       DASVIM010000058         6595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993 k141_21776,
            whole genome shotgun sequence.
ACCESSION   DASVIM010000058 DASVIM010000000
VERSION     DASVIM010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21776"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="VF305_01170"
     CDS             complement(<1..133)
                     /locus_tag="VF305_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEX7415775.1"
                     /translation="MKLLMHICCGPCTIYPLKELRIQGHEVTGVFYNPNIHPYQEYQK
                     "
     gene            complement(305..1333)
                     /gene="ruvB"
                     /locus_tag="VF305_01175"
     CDS             complement(305..1333)
                     /gene="ruvB"
                     /locus_tag="VF305_01175"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX7415776.1"
                     /translation="MDTPIKNPLISPSCTEEEYKLEVTLRPAKLNDYVGQEKVKSNLA
                     IFIEAAKKRREALDHVLLYGPPGLGKTTLAYIIAKEMGVDIKSTSGPVIERPGDLAAI
                     LTNMQEQEILFIDEIHRLSHVVEEILYPAMEDFHIDILIGQGPSARSMKLDIPKFTLV
                     GATTRAGSLTSPLRDRFGMHFRLEFYSPQELAIIIKRSASILGVKLAAEGAEELARRA
                     RGTPRIANRLLKRVRDYAEVKADGTIDGKIAREALDAFEVDQKGFDQMDRKLLLTLIE
                     KFNGGPVGVENLAAAIGEEKITIEDVYEPYLIQEGYLQRTARGRIASARAYEHFGLKA
                     PTGQKELF"
     gene            complement(1357..1965)
                     /gene="ruvA"
                     /locus_tag="VF305_01180"
     CDS             complement(1357..1965)
                     /gene="ruvA"
                     /locus_tag="VF305_01180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX7415777.1"
                     /translation="MIALISGKIAYKGISHVIVDTQGVGYRIFIPLTTFYELPAAGQN
                     VILHVHTHVKQDAINLFGFYTLQEKELFQLMISVSGIGPKIAMNILSGISAQELLRAI
                     SRGDVGKLVSIPGVGKKMAERLILELKEKVIKKMLMEKMPAADDQHKANEIIIEDVLS
                     ALVNLGYKSNIAKDALDKVLQSSEKELVMDQLLKKTLKILAG"
     gene            complement(1965..2444)
                     /gene="ruvC"
                     /locus_tag="VF305_01185"
     CDS             complement(1965..2444)
                     /gene="ruvC"
                     /locus_tag="VF305_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX7415778.1"
                     /translation="MRILGIDPGSRITGYGIIEKQGNYLRHVIHGEVKPRKDSLLSIV
                     LSSIYQQLSAVIERTAPQAISLENIFYGKNVRSLIKQAQVRGVVIFAGADKGIPIFEY
                     SPLEVKKAVVGYGRAEKRQVQVMVKAILKLPVLPPADAADALAAAICHANFLKEQPI"
     gene            complement(2477..3226)
                     /locus_tag="VF305_01190"
     CDS             complement(2477..3226)
                     /locus_tag="VF305_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7415779.1"
                     /translation="MSGHSKWSTIKRKKGAIDAKRGKMFTKIIKEITLAARLGGGDIE
                     GNSRLRQAVLAAKEENMPKDNIERAIKKGIGGGEGAANYEEITYEGYGPGGAAVLVEI
                     MTDNKNRTVAEIRHIFSKHGGNLGENGCVAWMFTKKGSIVIDKKAIDEDTLMELALEA
                     GAEDVKSEDDEYEVITSPASLETVKKAIEAKGIKHLAARIDMISSNTVRLEANKAEQM
                     LKLMEKLEDNDDVQNVYANFDIDKDVMEKLS"
     gene            complement(3487..4668)
                     /locus_tag="VF305_01195"
     CDS             complement(3487..4668)
                     /locus_tag="VF305_01195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX7415780.1"
                     /translation="MREVVIVSGARTAIGDYMGSLSGLTPVQLGVTVLKAAIAKTGID
                     KNLVQEVVAGQCNQAGAPGNSARHIALGAELPVESFAFTVHQQCPSSMRASELLSQEI
                     MLGKIDIGAVVGIESMSNAPYLLFGARKGYRLADGEKIQDSLMIGGLVDALLGYHMGI
                     TADNIADQYKISRQEQDEWALMSHQRACAAIEKGWFKDEIVPVEIKTKKGPAIFDKDE
                     HPRADTSMESLGKLKPAFKKDGTVTAGNASGLNDAGSCMIMMAADTAKKMGIKPIARV
                     VASAPGSVEPRIMGMGVVPAVRNALKFANMKQGQIDYFEFNEAFAAQIIGCNRELKIP
                     LDKINAVGSGISMGHPVGATGARLIITMINEMKRRGVKYGCASLCASGGPGHAFIVES
                     I"
     gene            complement(4927..5736)
                     /locus_tag="VF305_01200"
     CDS             complement(4927..5736)
                     /locus_tag="VF305_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX7415781.1"
                     /translation="MLTFYIVDVFAEEKFTGNQLAVFCGGMRLSSDQMQSIAREMHFS
                     ETTFIMSEEKRNGGYDVLIFTPSAEVPFAGHPTLGTAHIIRSEIATDAAREIVLNLKV
                     GPIPVTFQEEKKGTISWMKQVNPEFGGIIGADTIVHVLGLRRSEIDERFPVEEVSTGL
                     PHIIVPLKSLDSLKRAHINKDKYFDLIKNTWAKPILIFCPESHRGLNDISVRMFADYY
                     GVPEDPATGSGNGCLAAYLVKYRYWGKNEIDICSEQGYEINRPSLLYLKAQ"
     gene            complement(5812..>6595)
                     /locus_tag="VF305_01205"
     CDS             complement(5812..>6595)
                     /locus_tag="VF305_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7415782.1"
                     /translation="GASRLAIAGIVLLAFAYIRKNKLIITAKELLIISISSVFLWTGG
                     NGLLLWAEQHTYSGLAALLVSTTPMWAALLDSIFSKKTPSSLLIGSIFLGLGGIAVLI
                     YPSLRAGNSADYISGLLLVGASLCWAAGSVYQKRNPIDVPDTVIAGYQTIIGGCSFLI
                     LSLLCNEPTPQPTQNAWLAWGYLIVFGSVFAFTSYVRALRLLPINIMMTYAFVNPVLA
                     VFLGWLILGEKITNWTIAGAALVVLGVFGVFREQQKIGERIN"
BASE COUNT         1827 a         1568 c         1262 g         1938 t
ORIGIN      
        1 gtttttgata ttcctgatag ggatgaatat tcgggttata aaaaacacct gtaacttcat
       61 gcccttgaat tcttaattct ttcaagggat aaattgtgca aggcccacaa cagatgtgca
      121 tcaatagctt catataatca atggttggaa tccagaacaa gccctggact caaaatccac
      181 cccaaggggt ggggtattag gttacgtgtg acctcgtgtg accttcaggt tattaggtta
      241 tgcatttcgc ttttgaaatt tgaatctcac aaataaaagt cgccttaacg acagcaactt
      301 gatattaaaa aagttccttc tgtcctgtgg gggccttaag gccaaagtgc tcgtatgctc
      361 tagcgctagc gattcttccc cgtgccgttc tttgcaaata accttcctgg attaaatacg
      421 gctcataaac atcttcaatg gttatctttt cctcgccaat tgccgcggca aggttctcca
      481 cgccaacagg tccgccgtta aatttttcaa tcagcgtcaa gagtagtttt ctatccatct
      541 gatcaaatcc tttttgatca acttcaaagg catccagcgc ttcccgcgcg attttcccat
      601 caattgttcc atctgcttta acttcggcat agtcgcgaac acgtttgagc aagcggttag
      661 caatccgggg cgtgccacgc gcgcgcctcg ccaattcttc ggctccctca gcggcaagtt
      721 ttactcccaa aatagaagca gatctcttga taatgatggc gagttcttgc ggcgagtaaa
      781 actccaagcg aaagtgcatg ccaaaacggt cacgcagagg agaagtcagc gatccggctc
      841 gggttgttgc tcccaccaag gtaaattttg gaatatcgag tttcattgat cttgccgatg
      901 gtccctgccc gattaaaata tcaatatgga aatcctccat agctggatat aagatttctt
      961 caaccacatg ggagaggcga tgaatttcat caatgaaaag aatttcctgc tcctgcatat
     1021 tggttaaaat cgccgccaag tcacccggtc gttcaataac cggaccggaa gtgcttttga
     1081 tatcaacacc catttcttta gcaataatat aggctaaagt cgtttttccc aaacccggcg
     1141 gaccatacaa aagaacatga tcgagcgctt cgcgtcgttt tttcgccgct tcaataaaga
     1201 tagctaaatt gctcttaact ttttcctggc cgacataatc atttaatttt gccggccgca
     1261 aagtaacttc caatttatat tcttcttcgg tacaggaggg gctgatcaaa ggatttttta
     1321 taggtgtatc cattttttat ctctaattgt gtcaatttaa cctgcaagaa tttttaaggt
     1381 tttctttaaa agctgatcca taaccaactc tttttcggag gattgcaaaa ccttatctaa
     1441 agcatccttg gcgatgttgc ttttataacc taaattcacc aaagcggaaa gcacatcttc
     1501 tatgataatc tcattggcct tgtgttgatc atccgctgcc ggcattttct ccatcagcat
     1561 tttcttaata actttttctt ttaactcgag gatcaaacgc tcagccattt ttttgcctac
     1621 gccaggaata ctgacaagtt ttcccacatc gccacgggat atagcacgca gcaattcttg
     1681 cgcagaaata ccggaaagta tattcatagc aatcttcggt ccgataccac ttaccgaaat
     1741 catcaattga aaaagttctt tttcctgcaa agtgtagaag ccaaataaat ttatagcatc
     1801 ttgtttgaca tgagtatgga cgtgcaagat aacattttgt cccgctgcgg gaagttcata
     1861 aaaagtcgtc agcggaataa agatacgata accgacacct tgagtatcaa caatgacatg
     1921 tgaaatgccc ttataggcaa ttttgccact gattaaggct atcattagat cggttgctcc
     1981 tttaagaaat tagcgtggca tattgcagcc gccagagcgt cagcagcgtc ggctggcggc
     2041 aagaccggaa gttttaaaat agccttgacc attacctgca cttgcctttt ctccgcccga
     2101 ccgtaaccaa ctacagcttt tttcacttcc aatggtgagt attcaaaaat cggtatgcct
     2161 ttatctgcac cggcaaaaat taccacaccc ctcacttgag cttgtttaat caaactgcga
     2221 acatttttac cgtaaaaaat attttccagc gaaatagctt gaggcgcagt ccgctcaatc
     2281 accgcggata gttgctgata aatggagctt agcactatgg ataaaagcga atcttttctc
     2341 ggtttgactt cgccatggat gacatgccgc aaataattgc cttgtttctc aattatacca
     2401 tagccggtaa tacggctgcc tggatcaatt cctaaaattc gcaatctgtc taccgtaaaa
     2461 aagtaagttg aatttgttag cttagttttt ccatcacatc tttatcaata tcgaaattag
     2521 cgtatacatt ttgtacatca tcattatctt cgagcttttc catcagcttc aacatttgtt
     2581 ccgctttatt tgcttctaat ctcactgtat tagacgaaat catgtcgata cgggctgcta
     2641 gatgttttat ccctttggct tcaattgctt tttttacagt ttccaaagag gctggagaag
     2701 taatcacttc atattcatcg tcttcgcttt tcacatcttc cgctccggct tccaaggcta
     2761 gctccataag agtatcttca tcaatcgctt ttttatcaat aacaatgctg ccttttttgg
     2821 taaacatcca agctacgcaa ccattctcgc caagattgcc gccgtgctta gaaaagatat
     2881 gtctaatttc cgctacagtt ctatttttgt tgtcagtcat aatttcgacc aaaacggccg
     2941 cgccaccggg gccataacct tcataagtta tttcttcata gttggctgct ccttcgccgc
     3001 cgccaatgcc ttttttgatc gccctttcga tattatcctt gggcatgttt tcttctttag
     3061 cagccagaac cgcttgccgg agccgagagt ttccttcgat atcgccaccg cccagccttg
     3121 ccgccaaggt tatttctttg atgatcttgg taaacatctt accacgttta gcatctatcg
     3181 cacctttttt cctttttatt gtgctccatt tagaatggcc ggacatgttc tttctcctgc
     3241 atagtttttc gatatttttt tgcctaataa cacaaggcaa tagtcttgta aagttaatta
     3301 catcgtcctg tgcgggaaag aattttcttt atttgttaca attttatttt gataatcaat
     3361 tatggcttaa gtatatttag agaaaaatca ggattaaagg gtatcgatca tgtaatattt
     3421 ttctgaatga aataatcccc cgtgaccagg tgccacgagg gattagtagc ttataaatta
     3481 ttctatttaa atagattcaa ctataaacgc atgaccggga ccaccgctgg cgcaaagaga
     3541 agcacaacca tattttacgc cgcgccgttt catttcatta atcatggtga tgatcaacct
     3601 ggcgccggtt gcgccgaccg gatggcccat gctgatgccg gaaccgacgg cattgatttt
     3661 gtccagaggg atctttaact cacggttgca gccaatgatc tgagcggcaa aagcttcatt
     3721 gaattcaaaa taatcaattt gcccctgctt catattagcg aattttaggg catttcgtac
     3781 agctggaaca acgcccatac ccatgatgcg gggctcaaca gagccggggg cggaagcaac
     3841 tactctggcg atgggtttaa tacccatttt cttggcggtg tcggcagcca tcatgatcat
     3901 acaggaaccc gcatcgttca ggccggaagc gttgccggcg gtaacggtac catctttttt
     3961 gaaagcgggt ttgagtttgc ccaagctttc catgctagtg tcggctctgg gatgttcatc
     4021 cttgtcgaat atggccgggc cttttttggt tttgatttcc accggcacga tctcatcctt
     4081 gaaccagccc ttttcaatgg cggcgcaggc gcgttgatgg ctcattagag cccattcatc
     4141 ctgctcctgg cgggaaattt tgtattggtc ggcaatatta tcagccgtga tacccatatg
     4201 ataacccagc agtgcatcga ccagaccgcc gatcatcaaa ctgtcctgaa tcttttcacc
     4261 atcagccaaa cgataacctt tacgggcgcc gaagagcaga tagggggcgt tgctcatgct
     4321 ttcaatgccg accactgcgc ctatgtcaat cttacccagc ataatctcct gtgagagtaa
     4381 ttcggaggca cgcatggaag aggggcactg ctggtgaacc gtaaaagcga aggactccac
     4441 cggcagttcc gcacccagag caatgtgacg ggcgctattt cccggtgcgc cggcctggtt
     4501 acactgaccg gcaaccactt cctgaaccag gtttttgtcg ataccggtct tggcgatggc
     4561 cgcctttaag acagtcacac ccaattgcac aggagtcaaa ccagaaaggg accccatata
     4621 atctccaatt gctgttctgg caccactaac aattacaact tctctcatag caaattcctt
     4681 tcttctatta tttataattt tccaatagaa aatggttttt atttatagaa agaccacata
     4741 tcccaatgcg tttttctaac agcaaaagaa tcacctttta tagttgacgt ttaatatcat
     4801 ttttcaatta ctataccaag aagaaaaaaa cgagcggaaa taatgattca aaatttattc
     4861 caaaaagttg cctttagcga tggtcgtcga tttacccccc acatatacat ctattcgccc
     4921 gttaatttat tgagccttta gatataatag agaaggacga ttaatttcgt atccttgttc
     4981 ggaacaaata tcaatttcat tttttcccca ataacgatat ttcaccaaat aagcagcaag
     5041 acaaccgttg ccgctcccgg ttgccggatc ttctggtacg ccataataat cggcaaacat
     5101 gcggacgctg atatcattta atccccgatg tgattcaggg cagaagatca gaatcggctt
     5161 ggcccatgta tttttgatca aatcaaaata tttatctttg tttatatgag ctcgcttcaa
     5221 tgaatccaaa cttttcagcg gaacaattat atgcggtaaa ccggtggaca cttcctcaac
     5281 cggaaaacgt tcatctattt ctgatcgtct aagtcccaat acatgaacta ttgtatcagc
     5341 accaataatc ccgccaaatt ccggattcac ctgcttcatc caggatatag tgcctttttt
     5401 ctcttcctgg aaagtaaccg gtattggccc gacttttaaa tttaatacaa tttctctcgc
     5461 agcatcagtt gctatttcac tgcgaatgat atgggctgta cccaaagtcg gatgcccggc
     5521 aaaaggaact tccgctgacg gtgtaaagat aagcacatca taaccaccat tgcgcttttc
     5581 ttcagacatg ataaaagttg tttcggaaaa atgcatctcc cgggcaattg attgcatttg
     5641 gtctgacgaa agcctcattc ctccgcaaaa tacagccagt tgatttccgg taaatttttc
     5701 ttccgcaaaa acatcaacaa tataaaaagt aagcatttcc atctgttgat tcctcaacaa
     5761 tacctaaata tagtttgatt atttatatct taattcttgc tagtcgcaat tttaattaat
     5821 tctttctcca attttttgtt gttctctaaa aacgccgaat acgcccaata ctaccaaagc
     5881 agctcccgct atagtccagt ttgtaatttt ttcaccgagt atcagccaac ctaaaaacac
     5941 ggccaagacc ggattcacaa aagcatacgt catcataata tttatcggca atagtcgcaa
     6001 ggctctaaca taagacgtga aggcaaacac agaaccgaag acgataagat atccccaggc
     6061 cagccatgca ttttgcgtcg gctgcggtgt gggctcatta cacagcaaag acaaaatcaa
     6121 aaatgaacaa ccgccgatta tagtctgata acctgcaatt actgtatcgg gaacatctat
     6181 cggattacgc ttttgatata cggaacctgc tgcccagcaa agcgatgcgc ctactaataa
     6241 taacccggaa atataatctg cggaattgcc tgcgcgaaga gacggataaa tcaaaacagc
     6301 aattccacct aatccaagaa agatagaacc aatcaataat gaagatggcg ttttctttga
     6361 aaaaatagaa tccagcaacg ccgcccacat cggtgtcgtc gaaacaagaa gtgctgctaa
     6421 gcccgaatag gtgtgttgtt ccgcccagag aagtaaccca ttaccacctg tccaaagaaa
     6481 tacactggag atggaaataa tcaacaattc cttagcagta ataattagtt tgttttttct
     6541 tatgtaagca aaagctaata atactatccc ggcgatggcc agccggcttg ctccc
//
LOCUS       DASVIM010000059         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1131824, whole genome shotgun sequence.
ACCESSION   DASVIM010000059 DASVIM010000000
VERSION     DASVIM010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131824"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..386
                     /gene="rpmB"
                     /locus_tag="VF305_01210"
     CDS             189..386
                     /gene="rpmB"
                     /locus_tag="VF305_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX7415783.1"
                     /translation="MSRVCEVCGKKPAIGNSVSHANNKTKTVWYPNLQRLRCVDEKTG
                     SVKRVRVCTRCIRSGFVKKAM"
     gene            complement(379..1179)
                     /locus_tag="VF305_01215"
     CDS             complement(379..1179)
                     /locus_tag="VF305_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415784.1"
                     /translation="MLMLTHTYLLQKVLGSADIKNKDLDVYVYNIVPDLLTIHSQISS
                     SKTHSIKRTLQIPADYPRSAYVMFHLLVDDLAHYGYICQEGKEKFDPDSQGYSYIKGK
                     PLINSILELYNTIQKDISYNEAVYRSHLIIEMIYDLVILNQINSLKTVDLLAEAIDFT
                     AKNKIDEFTATVNQLYDVGESKIKEVMKEASFYITKERMVSIMNIEGRIRLYTDKFGL
                     KSNNQLFFDGVKNLFLRAKDSLELDDDELFLRETTQTIKNYGWLPPLT"
     gene            complement(1173..1424)
                     /locus_tag="VF305_01220"
     CDS             complement(1173..1424)
                     /locus_tag="VF305_01220"
                     /inference="COORDINATES: protein motif:HMM:NF027581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HEX7415785.1"
                     /translation="MRNHTKMIKKYFKINRRNIAIVQFIIEGYEGMATVSTIDPHTAI
                     IKILIMPDFISEFSGLIDDLKNKYKIVEINNCKEENNLC"
     gene            1562..3301
                     /gene="recJ"
                     /locus_tag="VF305_01225"
     CDS             1562..3301
                     /gene="recJ"
                     /locus_tag="VF305_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX7415786.1"
                     /translation="MTRTKKLATTQWKLKESVNKDIEDSLSKEFGIYPIISQILANRE
                     ILDMEGARRYLYPSLNDLHSPLLMKDMKKGVSRLLKAIHEHEEIVIYGDYDADGITSV
                     VILYKFIKEITPHVTYYIPDRVQEGYGLKIPVIDKFKGNNVKLIITVDCGISDAEQIA
                     YAQSIGIDTIVLDHHEISGALPPAVASINPNREDCIFPFKDLAGVGIAYNFLIALRGS
                     LRKEGFWKNKDYPNLKDYLDIVALGTIGDVAPLVDENRIFAKIGLELITEGKRPGIKA
                     LKEVSGVDGQAIDSFKASFCLIPRINAAGRIASALDAVELLLSENIDEARLLAEKLDS
                     YNRRRQSMEKNILKDILEKINNNPDIEKMHALVFASDKWHPGVVGIVASRLVDLFSRP
                     AFVISLKNGVGKGSGRSVSDFNIYKGIQQCASLLLSYGGHYRAAGISIKEADIAEFAS
                     LLDEIIRNTVQSPELVSPTLIDSECLLTDINLNLISQIEMLAPFGSKNPEPVLCARNI
                     KAASPTIVGNNHLKMRLNGNGMSCDSIWFNMGNHLSALTGANLDVAFTPQINYWNGAS
                     EIQLKMKDVIILP"
     gene            3333..3680
                     /locus_tag="VF305_01230"
     CDS             3333..3680
                     /locus_tag="VF305_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415787.1"
                     /translation="MNLATKAALLSALLFPGWGQIYLKKYKRGLAFIVPVFFCILFLV
                     WAVVQVAIKVLKATPVKKGTVNFNAVVNLAVNSIKELDLFYLLLILIFMILLSIFSII
                     DAYLLGKKAGIKS"
     gene            complement(3816..>4079)
                     /locus_tag="VF305_01235"
     CDS             complement(3816..>4079)
                     /locus_tag="VF305_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415788.1"
                     /translation="IAPSQLKMISGKFNNLPLQAVVTRKGHEDILKLRVEKLLPEIGS
                     PAWEEGFKTIFREICTVKIDQLEYLSPGVIKPDEKLIVDQRQW"
BASE COUNT         1212 a          795 c          789 g         1283 t
ORIGIN      
        1 gcgcgccttg catctgaaca tttttgaaca gccccaaaac atggtgtttt tcaccgatca
       61 cacgctgtaa atttatcgtt ttgagccttt ttacgagttc atcaaaaatt aaattgaagt
      121 gaaaaaatgc ttgcaaaatg acatcttatg gtttagtaac gacgcacagt tttaggagga
      181 tttttttcat gtctcgtgtt tgcgaagtat gcggtaaaaa accggcaatt ggtaacagtg
      241 taagccatgc taataataaa acaaaaacag tgtggtatcc caatttgcag agattacgct
      301 gcgttgatga aaaaaccgga tcggtaaaaa gagtaagagt ctgcactcgt tgcattcggt
      361 ctggatttgt aaaaaaggct atgtaagtgg cggaagccaa ccataatttt ttatagtttg
      421 ggtggtttct cgaagaaata actcatcatc atcaagttcc agcgaatctt tagctcgtag
      481 aaacaaattt ttaacaccgt caaaaaataa ttgattattg cttttgagac cgaatttatc
      541 tgtataaaga cgaattcggc cttcaatatt catgatgctc accattcttt ctttagtgat
      601 gtagaaggaa gcctctttca tcacttcttt aattttactt tctcccacat catagagctg
      661 gttaactgtg gccgtgaatt cgtcaatttt attttttgcc gtgaaatcta tcgcctccgc
      721 caaaagatca acagttttaa gtgaatttat ctgattcaag atgacaagat catagatcat
      781 ttcgataata aggtgggaac ggtaaaccgc ttcattataa gaaatgtcct tttgaattgt
      841 attgtataat tccaaaatag agttgatcag aggtttcccc ttgatatagg aatatccttg
      901 agaatcaggg tcgaattttt ctttgccctc ctggcaaata tatccgtaat gagcaaggtc
      961 atcgactaaa agatgaaaca tcacataagc cgatcgggga tagtccgccg gaatttgcag
     1021 agttcttttt atactatgag ttttgctgga acttatttgc gagtgaattg ttaataaatc
     1081 cggaacaata ttataaacat aaacatccaa atccttgttt tttatatctg ccgaacccaa
     1141 aactttctgc aaaagatatg tatgggttaa catcagcata aattattttc ctctttacaa
     1201 ttattgattt ccacaatttt atatttattc tttaaatcat caatgagacc gctaaattcc
     1261 gaaataaaat caggcataat caaaattttt attatcgcgg tgtgaggatc aatcgttgat
     1321 accgtggcca ttccttcata cccttcgata ataaattgta ctatggcaat attgcgtcga
     1381 tttattttaa aatatttttt tatcatctta gtatgattcc gcattaccac tgatctccgt
     1441 aaagatcaag attaacggcc aattgacgta tgcagataaa cactgtatga ttaataaaaa
     1501 acttgtggtg atatatgcgt atcagtagat ttatactaat ctttttaatg aagataaaac
     1561 aatgacgaga actaaaaaac tggcgacaac tcaatggaaa ttgaaagaat ccgtcaacaa
     1621 ggatatagaa gattctttga gtaaagaatt tggcatttat ccgattatat cccaaattct
     1681 tgctaacagg gaaattctgg atatggaagg cgcccgccgc tatctctatc cttctctaaa
     1741 tgatctgcat agccctcttt taatgaaaga tatgaagaag ggggtttcgc gtctgttgaa
     1801 agcgatccac gaacacgaag aaatagttat ttatggcgac tatgatgctg atggcataac
     1861 ttccgtagtt attctttata aattcattaa agaaataacg cctcatgtaa cttattatat
     1921 tccagacaga gtacaggaag gttacggctt aaaaattccg gttattgaca agttcaaagg
     1981 caataacgtt aagctgatta ttaccgttga ttgcggtatt tccgatgcgg aacaaattgc
     2041 ctatgctcaa tcaatcggca ttgatacgat tgttctggat caccatgaga tatcgggagc
     2101 acttcctccc gccgtcgcct ccataaatcc aaatcgcgaa gactgtatat tccctttcaa
     2161 agatctggcc ggtgtgggca ttgcctataa ctttctcatc gctttacgcg gcagtttgcg
     2221 taaggaagga ttctggaaaa acaaagatta tcccaattta aaagattatc tcgatattgt
     2281 cgctttagga actattggcg acgtcgcgcc gcttgtcgat gaaaacagaa tttttgccaa
     2341 aatcggtttg gaactgatta ccgaaggcaa gcggccggga atcaaagctc ttaaagaagt
     2401 cagcggcgtt gacggtcaag cgatcgattc ttttaaagct tctttttgtc tcattccccg
     2461 cattaatgcc gccggccgca tcgcttctgc tctggatgca gtggaattgc ttttgtctga
     2521 aaatattgat gaagctcgcc tattggccga aaagttggat tcctataacc gccgccggca
     2581 atcaatggaa aagaatattt taaaagatat cttagaaaaa ataaataaca atcccgatat
     2641 tgaaaaaatg catgcacttg tttttgcgtc cgataaatgg catccgggag ttgtcggcat
     2701 tgtcgcttcc cgtctcgttg accttttcag ccgtccggca tttgttatca gtttaaagaa
     2761 cggtgtgggc aaaggttccg gaagaagtgt ttccgatttt aatatttaca agggaattca
     2821 gcaatgcgcc tctcttctgc tttcctacgg cgggcactat cgcgctgcgg gaatctctat
     2881 aaaagaagct gatattgctg aatttgccag ccttttagat gaaataattc gtaatactgt
     2941 gcaatcgccc gaacttgttt ccccaacgct tattgactcg gaatgcctac ttacggatat
     3001 caatctgaat ttaattagcc agatagagat gcttgcgcct tttggcagta aaaatccgga
     3061 acccgtgctt tgtgcccgta acattaaagc tgcttctcct actattgtcg gcaataatca
     3121 tctaaagatg cggcttaacg gcaatggcat gtcttgcgat tccatctggt tcaatatggg
     3181 caaccaccta agtgcactta ccggtgccaa tcttgatgtt gcttttacac cgcaaatcaa
     3241 ctactggaat ggcgcttccg agattcagct aaagatgaaa gatgtaatta ttcttcctta
     3301 aattgtttct tcaacaaaag agagaacaca atatgaattt agcaactaaa gcagcattat
     3361 taagcgcgct tcttttccca ggctggggac agatttattt aaaaaaatac aaacgcggac
     3421 tagcgtttat agtacctgtt tttttctgta ttttgttttt agtctgggct gttgttcaag
     3481 tggcaattaa ggtattaaaa gctacacccg ttaaaaaagg aactgttaat tttaatgccg
     3541 ttgttaacct ggctgtcaat tctataaaag aactggactt gttttatctt cttttgattt
     3601 taatttttat gattctctta tcgatttttt ccatcattga cgcctatctg ttaggaaaaa
     3661 aggctggtat aaaaagctga tgaacccgta aaaagtcgaa aatgtccttg acgcgtcatt
     3721 ccggcgaagg ccggaattca gtgattataa atagttataa aaattctaga ccccggcctt
     3781 cgccggggtg acgacttgtt acgaggtcgt taaggctacc actgccgttg atcaacaatc
     3841 aacttctcgt ccggtttaat aacccctggg ctcaaatatt ccaattgatc aatttttact
     3901 gtgcaaatct cgcggaaaat agttttaaac ccttcttccc aggcagggct gccgatttcc
     3961 ggtagcaatt tttccacgcg taattttaaa atgtcctcat gtcctttgcg ggttacaact
     4021 gcctgcaaag gtagattatt gaatttaccc gaaatcattt tcagttgact gggtgctat
//
LOCUS       DASVIM010000060         4266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1473454, whole genome shotgun sequence.
ACCESSION   DASVIM010000060 DASVIM010000000
VERSION     DASVIM010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473454"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VF305_01240"
     CDS             complement(<1..735)
                     /locus_tag="VF305_01240"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX7415789.1"
                     /translation="MLEKIRIFSGNANIALATKICKNLGVSLGKANVTTFSDGETRVE
                     INENVRGMDVFIIQSTCSPVNVTCMELLIMIDAMKRASADRITAVVPYYGYGRQDRKV
                     APRAPITAKLLADLITTAGANRVLSMDLHAGQIQGFFNIPVDNLFATPVLLDYMKNIY
                     LDNTDNTVVVSPDTGGVERARAFGKRLGASLAIIDKRREGPNEAQVMNIIGNVQGKRV
                     IILDDMVDTAGTVVQAANAMKEAGALE"
     gene            complement(761..835)
                     /locus_tag="VF305_01245"
     tRNA            complement(761..835)
                     /locus_tag="VF305_01245"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Gln,seq:ttg)
     gene            complement(885..1724)
                     /gene="ispE"
                     /locus_tag="VF305_01250"
     CDS             complement(885..1724)
                     /gene="ispE"
                     /locus_tag="VF305_01250"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX7415790.1"
                     /translation="MLWKAPAKINLFLRVLRKRNDGYHDIASLLQKITLYDELIFSPR
                     PEGITLNCPGSNLPATEDNLAIRAAQAIFAYSHYPSGMKITLTKKIPLAAGLGGGSSD
                     AATTLMALNEICSLGLKKQELMLMGAKLGADVPFFIFGNTALASGIGDELKDLQNLPK
                     LNLVLINPPFELSTKMVYENLNLRLTREKINYSIPRFFVLGDVVRELHNDLEEVSLKM
                     HPELNDLKQKLLRHGALGALMSGSGPTVFGIFTDEKAAKKALETIKKEVSGECIVCFA
                     QSL"
     gene            complement(1726..2136)
                     /locus_tag="VF305_01255"
     CDS             complement(1726..2136)
                     /locus_tag="VF305_01255"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEX7415791.1"
                     /translation="MKEKKKKDSGFVVKDKRLFAESGEPRREEEPQAPAAEEAPKIQE
                     SSPTAPEQVSEYPPITFTNFVLSLSTSALFHFGDFPDPEGGTAQKDMPAAKQTIDILD
                     MINEKTKGNLNENENSLIQGVLYELKMRYVKEKA"
     gene            complement(2175..3026)
                     /gene="htpX"
                     /locus_tag="VF305_01260"
     CDS             complement(2175..3026)
                     /gene="htpX"
                     /locus_tag="VF305_01260"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX7415792.1"
                     /translation="MNNTVKTALLLGALTGLLMFIGGLIGGKGGVYIAFIFAIILNFG
                     SYWFSDKIVLKMYHAQEVSESSAPDLFSIVRNLALKAQLPMPRVYIIPEETPNAFATG
                     RNEKHAVVAVTEGIRRILNKEELEGVIAHELSHIKNRDMLISSIAATIAGAIVMLAHM
                     AQWAAIFGGGRSSDDDSGGGIIGLIAMAIFAPLAAAVIQMTISRSREYMADAGGAGIT
                     KNPYGLASALEKLTKASQIILLDAKPSTAHLFIVNPLAGKSLMNLFSTHPPLADRVAR
                     LRSMKPY"
     gene            complement(3038..>4266)
                     /locus_tag="VF305_01265"
     CDS             complement(3038..>4266)
                     /locus_tag="VF305_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7415793.1"
                     /translation="GSLKEMKLHWSSEKDIITKIQHVKSQMESLKTEEVNAQREGNLA
                     KAAEIRYGKLVALDKELQEDQTKLLETQKNKKMLKEEVDAEDVAEVVSTWTGIPLARM
                     MESDVQKLIHMEGRLKMRVIGQDEGIQAVSAALRRARSGLQDPNRPIGSFIFLGPSGV
                     GKTELARALAEFMFDNEQAMVRIDMSEYMEKHAVARLIGAPPGYVGYDEGGYLTEAVR
                     RKPYCVLLFDEIEKAHPDVFNILLQILDDGRLTDGHGRTVDFKNTIVIMTSNVGGQWI
                     QDSTLDEKEKQNRTMEALRATFKPEFLNRIDDIIIFRALTLEDINRIVDIQLALIDKR
                     LKDRKLSIELTDSAKKYIANEGYSPVYGARPLKRSLQKLILDRLSMEILSGKFTEGDH
                     VVISLSKGELIFNKKS"
BASE COUNT         1078 a         1010 c          931 g         1247 t
ORIGIN      
        1 ttccagggcg cctgcttcct tcatggcgtt ggcggcctgc actacagttc ctgctgtatc
       61 gaccatatca tcaagaataa ttactctttt gccttgcacg ttaccgatga tattcatcac
      121 ctgagcttcg ttggggcctt ctcttctctt atcaataatg gcgaggcttg ctcccagtct
      181 ttttccaaat gcccgagcac gttctacacc gccggtatct ggcgaaacga ccacagtatt
      241 atcagtatta tccagataaa tatttttcat ataatccagt aaaaccggag tagcaaaaag
      301 attgtccaca ggaatattaa aaaatccctg aatctgaccg gcgtgcagat ccatggagag
      361 cacgcggttg gctccggcag tggtaatgag atcggccaat aattttgcgg taattggtgc
      421 tcgcggggcg actttcctat cctgccggcc atagccataa taaggtacca cggcggtaat
      481 ccggtcggcg gaagcacgct tcatagcgtc aatcataatg agaagttcca tgcaggtgac
      541 gttcaccggc gagcatgtgg attggattat gaaaacatcc atgccgcgca cattttcatt
      601 tatttctacg cgggtttccc catcactgaa agttgtaacg ttggctttac ctaaagatac
      661 tcccaaattt ttgcaaatct ttgtagccag cgcaatgttg gcattaccgg aaaatatcct
      721 aatcttttct aacattttaa acctttccca atataatttt tggctggggc gggaggattc
      781 gaacctccga gtgcaggaac caaaatcctg tgtcttaccg cttgacgacg ccccaatatt
      841 ttcaataaac aattactttt aacttgagac ggaaaataca tcaattacag tgattgggca
      901 aaacacacta tacactcacc ggaaacttct ttttttattg tttccaaggc tttttttgct
      961 gctttttcgt ctgtgaatat gccaaagacc gtggggccgc tgcccgacat caatgcacca
     1021 agcgcgccgt gcctgagcaa cttttgcttt aaatcattta attccggatg catttttaac
     1081 gaaacttctt ctaaatcgtt atgcaattcc cggactacgt cacccagtac aaaaaaccgc
     1141 ggaatgctat aattaatttt ttcccttgtc aatcttaaat tgaggttttc ataaaccatt
     1201 tttgtcgaaa gctcaaaggg gggattgatc aagaccagat tgagtttggg taaattttga
     1261 agatctttca gctcatcgcc aatgccggaa gctaaagccg tgtttccaaa aataaaaaag
     1321 gggacatccg cccccagctt tgctcccatc aacatcagtt cttgtttttt caaccccaaa
     1381 gaacatattt cgtttaaggc cattaaagtt gtagcggcat cggaactgcc accgccaaga
     1441 ccggcggcca gagggatttt tttagtaagc gtaattttca tgccggaagg ataatgggaa
     1501 taagcaaaaa tggcttgggc agcacgaatt gctaaattat cttcggttgc tggcaaattg
     1561 ctcccgggac aattaagggt aattccctcc gggcgaggag aaaagatcaa ctcatcgtaa
     1621 agagtaattt tttgcaggag ggaagcaatg tcgtggtatc catcgttacg cttcctcaga
     1681 acgcgcaaga aaaggttaat ttttgccggc gccttccaga gcatttcaag ccttttcttt
     1741 aacgtagcgc attttcagtt cgtagagaac tccctgaatt aaactatttt cgttttcgtt
     1801 gagatttccc ttggtcttct cattgatcat gtccaggata tcaatagttt gtttcgctgc
     1861 aggcatatcc ttttgtgccg tgccaccttc gggatcggga aaatcgccga aatgaaaaag
     1921 ggcggaagtg ctcagagaca ggacaaaatt agtaaaggta ataggtggat actcggaaac
     1981 ttgttccggc gcagttggcg aactttcttg aattttaggc gcctcttcgg cagcgggagc
     2041 ctgcggctct tcctcacgac gcggctcacc cgattcagca aaaagacgct tatcttttac
     2101 tacaaacccc gaatcttttt tctttttttc tttcattatt attcctccct ttaccgcgct
     2161 gcggaaaatt tcttttaata tggtttcatg cttcgtagac gggcaactct gtccgcaagc
     2221 gggggatgag tgctgaaaag attcatgagc gactttcccg caagcggatt gacgataaat
     2281 aaatgagcgg tggaaggttt agcatccagg agaatgattt gcgaagcctt cgtaagtttt
     2341 tccaacgcac tggccaatcc ataaggattt tttgtaatcc ctgcgccgcc ggcgtcggcc
     2401 atatattcgc gggaacggga aatggtcatt tgaatgacgg ctgcggccag aggggcaaaa
     2461 atggccatag ctattaaacc gattattccg cctccgctat catcgtcact gctgcggccg
     2521 ccgccgaata tagccgccca ttgagccatg tgagccagca taacaatggc gccggcaatt
     2581 gttgcggcaa tagaactaat cagcatatct ctgttcttaa tatgagataa ctcatgggcg
     2641 attacgcctt cgagttcttc tttatttaaa attcgcctta ttccctcagt tacggcgaca
     2701 acggcgtgtt tttcattacg gccggtcgca aaagcattgg gtgtttcttc gggaataatg
     2761 taaactcgcg gcatcggtaa ttgggctttt agagcgagat tgcgcacaat actgaaaagg
     2821 tccggtgccg agctttcgga aacttcctgc gcatgataca tttttaaaac aattttgtcg
     2881 gaaaaccagt aactgccgaa atttaaaatt atagcgaaga tgaaagcgat ataaacgcct
     2941 cccttcccac caatcaggcc gccgatgaac atcagcaggc cggttaacgc acccagtaat
     3001 agggcggttt ttacagtgtt attcatataa aactccctta agatttctta ttgaaaatta
     3061 attcgccctt cgacaaactg attacaacgt ggtcgccctc agtgaacttg ccggagagta
     3121 tttccatcga cagccggtca agaataagtt tctgtagtga ccgcttgagc ggacgcgcgc
     3181 cgtacacagg gctgtaacct tcattggcta tatatttttt tgctgaatct gtcaattcta
     3241 tgctcaattt tcgatctttg agtctcttgt caatcagcgc taattgaata tcaacgatac
     3301 gattaatgtc ttccagcgtc aaagcccgga aaatgataat atcgtcaata cggtttaaaa
     3361 attccggttt gaaagttgcg cgaagcgctt ccatagtccg gttttgtttt tctttctcat
     3421 ccaaagttga atcttgtatc cattgtccac cgacattgga agtcataatt acaattgtat
     3481 ttttaaaatc aactgtgcgg ccatgtccgt cggtgaggcg tccgtcgtcg agtatttgca
     3541 gtaaaatatt gaaaacgtcg ggatgagctt tttcgatttc gtcaaaaagc aggacgcaat
     3601 aaggcttgcg tcttacggcc tcagttaaat aaccgccctc gtcgtagccg acatagccgg
     3661 gcggagcgcc gattaaccgt gccacagcat gcttttccat gtattccgac atgtcgatgc
     3721 gtaccatagc ctgttcattg tcgaacataa attctgctaa agcgcgcgcc agttcggttt
     3781 tcccgacacc gcttggtccc aggaaaataa atgagcctat cggccggttg ggatcttgca
     3841 gtccggagcg tgcgcggcgc agcgccgcag aaaccgcttg aatgccttca tcctggccga
     3901 taacacgcat tttaaggcgt ccttccatat ggatgagctt ttgcacgtcg ctttccatca
     3961 tccttgccag aggaattccc gtccaggtag aaacaacttc agccacatct tccgcgtcga
     4021 cttcttcttt gagcattttt ttgtttttct gggtttccag cagttttgtt tgatcttctt
     4081 gtaattcctt gtccagagct accaatttgc cgtaacggat ttctgcggct ttagcgaggt
     4141 tgccttcccg ctgggcattg acttcttctg tttttaagga ttccatttgg cttttaacat
     4201 gctggatttt tgtaatgata tctttttcgc tcgaccagtg caatttcatt tccttcaagc
     4261 ttcctt
//
LOCUS       DASVIM010000061         4990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_108901, whole genome shotgun sequence.
ACCESSION   DASVIM010000061 DASVIM010000000
VERSION     DASVIM010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108901"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1408)
                     /locus_tag="VF305_01270"
     CDS             complement(65..1408)
                     /locus_tag="VF305_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX7415794.1"
                     /translation="MIKQLDLGKEAIFPLILKMSWPSIVAMIAMSLYNFIDTFWLARL
                     SSQALAALTVCFPIQMIFAAIGVGTGIGAGSFSARMFGAGKFLQAKQTAGQIFSLSFF
                     FSLIMIIFVLFFYDAILTFFGASDEIMPLCRDYLVIIVFSSPFLFFSMMSNNLLRVEG
                     RPLLSMYVVLVASISSAILDPFLIFGIGPFPRMGIKGAALAAVIAQFIACLFSIYFMQ
                     LKSSKYELKWEYLFPNISIINSIYSTGFPSIAANLIVSLVLVVYNHVLANFGSLAIAA
                     LGICFRVNSLVTMVLFGIGFGLMPIVGFNDGARLYQRLKKSVFVALKVSISFAAVSSI
                     ILEIFAPQIVGLFSNDPALLAIAIPALRIYVSVLILIAPIMMFINMFLGLGKGTLAMF
                     LLFFRDSILLIPLLIILSSSFGLLGAWLALPLANLFAFFAVLFWAKKDLRRFGIN"
     gene            1503..2414
                     /gene="miaA"
                     /locus_tag="VF305_01275"
     CDS             1503..2414
                     /gene="miaA"
                     /locus_tag="VF305_01275"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX7415795.1"
                     /translation="MAKNLIVILGTTASGKTELAAKLAYDLQGEIISADSRQVYKNMD
                     IGTGKDLSRYIINGKQIPYHLIDIVDPESEFNLFEFQGRFYKIFSELMEKEVLPILAG
                     GTGLYLESVLTGYNIPEVPQDQELRKDLNRKSKGELREILTALKPHLHNKTDLDDPER
                     LIRAIEIERARNIKVRDQQEKPYIDALIFGIHWERAALRQRITLRLKGRLEQGMIQEV
                     MKLHTGGLTWTKLESFGLEYRFISQYLQNKITLDEMTNKLGTAIHQFAKRQETWFRRM
                     EKKGIVINWLPGNDYPLLKEKVAKFLR"
     gene            2411..3763
                     /locus_tag="VF305_01280"
     CDS             2411..3763
                     /locus_tag="VF305_01280"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7415796.1"
                     /translation="MKKPSLIDTYLQNYATGEKWNLIANDTENISQVVVIPAYAEKEM
                     LFSTLVSIAQNPLSSLEYSFILCVINNKNNSPSAVINNNLQTIKYLEALVRKKPLRKF
                     NAAQELYPLLLTLSDAKLKFGYIDASSRGYEIPQNAGGVGMARKIGMDMALRLLKKSS
                     APRNLILSLDADTLVQSNYLSAIKNYFTQRVKTAIVAYEHQMPSNHEEQAAICCYEIF
                     LRYWVLGLKYSKSPWAFHSIGSTIIVSTEAYLEVRGMNKREAGEDFYFLNKLAKMGKV
                     GYIKETRVYPSARSSTRVPFGTGKRIQRFLSGKHQEYLLYDPQVFTILAQWLELMKSA
                     FDFSVDEIILKAEKINPALKSFLVRCDFVAVWSKILGNVKDEKTLARQFNDWFDGLKT
                     LKLINYLTYEAYPQIDMFEALGRILSLLGMPGLKRNLELKIPKLKEQIEIVQYLRIIT
                     "
     gene            3878..4951
                     /locus_tag="VF305_01285"
     CDS             3878..4951
                     /locus_tag="VF305_01285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7415797.1"
                     /translation="MKNITDKSWIAIFVLLAFVSGCGNEEKKKDTAVPMAGTNQIQPV
                     DSGSSAADSAKDAVAKQAESNDIVVSVDGNILKKAELEKNIKERLTILQDKIPAGKEQ
                     EARENIKKRLMDEFIMRTLLIKEVKKRKIAVSDKEIKSETDKIKTNLPPDKKVDEFLK
                     ENKISKEDIALGIKVEKLLKLELGKKAKPTQKEISKFYNDNIDKFFSVPENVHVRHIL
                     VTIDKGDDDKVKTEKKDKIENLRKQVLDGADFAEVARKNSDSPNKEKGGDLGNITRGQ
                     TVEPFENAAFSQEKNVIGPVVTTEYGYHVIQVLDHNPKKTIALDEVKSKISAHLEQQK
                     KAEAFALIMKKLQGNAKIIIYQN"
BASE COUNT         1796 a          872 c         1041 g         1281 t
ORIGIN      
        1 gaccagccat cttccctcag tttattaata ataaagattt tgggcatgat taacttaact
       61 gtgcttagtt gattccaaaa cgccgcagat ctttttttgc ccaaaagagg acggcgaaaa
      121 acgcgaagag gttggctaaa ggcagcgcca gccaagcgcc cagaaggcca aaagaggaag
      181 ataaaataat caaaagtgga ataagtaaaa tactgtcgcg aaagaaaagt aaaaacatgg
      241 ccaaagttcc tttgcctaat cccaaaaaca tattaataaa catcataatc ggagcaatta
      301 aaataagtac tgaaacgtaa atgcgcaacg cgggtatggc aatagccagt aaagccggat
      361 cgttggaaaa aagcccgact atctgcgggg caaatatctc taaaattatt gaagaaactg
      421 cagcaaaaga aattgatact ttcagggcaa caaaaaccga ttttttgagt ctttgataaa
      481 gcctagcgcc atcattgaac cccacaattg gcattaagcc gaagccgatg ccaaaaagta
      541 ccatagtaac cagactattg acacgaaaac atattcctaa agccgctata gccagggagc
      601 caaaattcgc cagaacatga ttataaacga ctaaaaccag gctgacaatt aaatttgcgg
      661 cgatagaagg gaagccggtg gaataaatcg aattaattat agaaatattg gggaaaagat
      721 attcccattt aagttcatat ttcgatgatt tcaattgcat aaaatatatg gaaaataggc
      781 aggctataaa ttgggcaatg acggctgcaa gtgcggcacc tttaattccc atgcgtggga
      841 aagggccgat accaaaaatt aaaaaaggat caagaatcgc gctggaaata gaggcgacta
      901 aaacaacgta catggataaa agaggtcttc cttccacacg aagtaaattg ttagacatca
      961 tcgaaaaaaa gagaaaaggc gaactaaaaa ctataataac aagatagtca cggcaaagcg
     1021 gcattatttc atcagaggcg ccaaaaaaag taagaattgc atcataaaaa aataaaacga
     1081 aaatgatcat gatcaaacta aagaaaaaag aaagggaaaa tatttggcca gcggtttgtt
     1141 ttgcttggag aaattttcca gcgccaaaca tgcgggcgga aaatgaaccg gcaccgatac
     1201 cggtgccaac accaattgcg gcaaaaatca tttgaatagg aaagcaaaca gtgagagcgg
     1261 ctaatgcctg tgagcttaaa cgtgccagcc aaaaagtatc aataaagttg taaagagaca
     1321 tggctatcat tgcgacaatt gacggccagc tcattttcaa aatcagcggg aagattgcct
     1381 ctttccctaa atccagttgt ttgatcattt tttcatagcc attattggta aaatattatt
     1441 agtatagcag agctttggtt gcttacacgc aaaaaatgat aatttcaagg caatagtaaa
     1501 atatggcgaa aaatctaatt gtgattttgg gcactacagc atcaggcaaa actgagttgg
     1561 cggcgaagct tgcttatgat ttgcagggag aaataatttc tgccgactcc cgtcaagtct
     1621 ataaaaatat ggatatcggc acgggcaaag atttaagccg gtatattatt aacggcaagc
     1681 agattcctta tcatctaata gatattgtag atccggagag tgaatttaac ttatttgagt
     1741 ttcaaggcag attctacaaa atattcagtg agctcatgga aaaggaagtt ttacctattc
     1801 ttgccggagg aaccggtctt tatttagaat ccgtgctgac agggtataat attcctgaag
     1861 tcccacagga tcaagaattg cgaaaagatt taaaccggaa atcgaaaggt gaactgcggg
     1921 aaatactcac ggcattaaag cctcatttac ataacaagac ggatcttgac gatccggaga
     1981 gattaatccg ggcaatagaa attgaacggg caagaaatat aaaagttcgg gatcagcagg
     2041 aaaaaccgta tattgatgct ctaatattcg gtattcattg ggaaagagca gcgctacggc
     2101 agcgtattac gttacggtta aaaggaaggc tcgaacaggg aatgatccaa gaagtaatga
     2161 agctgcatac cggaggattg acctggacaa aactggagag cttcgggttg gagtaccgat
     2221 ttatttctca atacttgcaa aacaaaatta cactagacga aatgacaaac aaattaggca
     2281 ccgctatcca tcaatttgcc aaaagacagg aaacgtggtt tcggcggatg gagaaaaaag
     2341 gaatagttat taactggctc ccaggaaacg actacccttt attaaaagaa aaagtagcga
     2401 aatttctgcg atgaaaaaac cttctttaat tgatacttat ttgcaaaatt acgcaactgg
     2461 tgaaaagtgg aatttaattg ccaatgatac tgaaaatatt tctcaagtcg ttgtgatacc
     2521 ggcttatgcg gaaaaagaaa tgctcttttc tactttagtt tctatagcgc aaaatccgct
     2581 ttcgtctttg gaatattctt ttatattatg cgttattaat aataagaata attctccttc
     2641 cgcagtgata aataataatt tacagacaat taaatatctc gaagcactgg tgagaaaaaa
     2701 gcctttaaga aaattcaacg ctgctcagga attatatcct cttttactta ctttgtccga
     2761 tgcgaaatta aagttcggct acattgatgc ttcgtccaga gggtatgaaa taccgcaaaa
     2821 tgccggcggc gtgggaatgg cgcgaaaaat cggcatggat atggctttgc gcttgctgaa
     2881 aaaaagttct gctccacgca atctaatttt aagtctcgat gccgatactt tagtccaaag
     2941 taattattta tctgcaataa aaaattattt tacgcaaagg gtcaaaacag caatcgtggc
     3001 ttatgaacac cagatgccat cgaatcatga agaacaagct gcaatttgct gctatgagat
     3061 ttttttacgc tactgggtgt tagggctaaa atattcgaaa tctccctggg catttcattc
     3121 cataggatca actataattg tttcgaccga agcatacctg gaagtaagag gaatgaataa
     3181 gcgcgaagcg ggggaagatt tttattttct gaacaaactc gctaaaatgg ggaaagttgg
     3241 ttatatcaaa gaaacacgcg tttatccttc ggcgcgttcc tccactcgcg ttccttttgg
     3301 aactggtaaa agaatacaaa ggtttctttc cggcaagcac caagaatatc ttttatatga
     3361 cccgcaagta tttacgatat tggcgcaatg gctagagctg atgaagagcg catttgactt
     3421 tagcgtagat gaaattatat tgaaagcgga aaaaattaat cctgccttaa aatctttttt
     3481 agtcaggtgt gactttgttg ccgtttggtc aaaaatactg ggcaatgtaa aagacgaaaa
     3541 aacgcttgcc cgtcagttta atgattggtt tgatggtctg aagactttaa aattgattaa
     3601 ttatcttact tacgaagcct atcctcaaat tgatatgttc gaagctctgg gcagaattct
     3661 ctccctgctg ggaatgcctg gattaaaacg aaatctagag ctaaaaattc ctaagctaaa
     3721 agaacaaata gagattgtgc aatatttacg tataataaca taatgataaa tatatcgaag
     3781 ttaacaaatc aggataatct tcttgacgga tatgcgcatt ttgcatatag ctcattcatt
     3841 aaaaaaataa tcgccaacta ttttcgggag gaaattaatg aaaaatataa cagataaaag
     3901 ctggatagcc attttcgttt tattggcctt tgtttccggt tgcggcaatg aggaaaagaa
     3961 gaaagatact gcggtcccca tggctggtac gaaccaaatt cagccggtag attccggatc
     4021 ttccgctgct gacagtgcaa aggatgctgt agctaaacag gccgaatcaa atgatatcgt
     4081 cgtcagtgtt gatgggaata ttttaaaaaa agcggagctg gaaaaaaata tcaaagaaag
     4141 gctcacgatt ctccaggata agattcccgc cgggaaagaa caagaagcac gggaaaacat
     4201 taaaaaacgg ttaatggatg aattcattat gcggaccctt ttaatcaaag aagtgaaaaa
     4261 gagaaaaatt gcagtgagcg acaaagaaat taaaagtgaa acagataaga taaaaactaa
     4321 tcttccgcca gacaaaaaag tagatgaatt cctcaaagaa aataagatat ctaaagagga
     4381 tatcgcttta gggatcaagg tcgagaaact tttaaagctg gaactcggta aaaaagcaaa
     4441 gcctacgcaa aaagaaataa gtaaatttta caacgacaat atcgataagt tttttagtgt
     4501 tccggaaaat gttcacgtcc gtcacattct tgtaactatt gacaaaggtg acgatgataa
     4561 agtcaaaacc gaaaagaagg ataagattga aaatttgcgc aagcaagtat tggatggagc
     4621 tgattttgcc gaagtcgccc gtaaaaattc cgattctccc aacaaagaaa aaggcggaga
     4681 tttaggtaat attaccaggg gacaaacagt agaacctttt gaaaatgccg ccttttcgca
     4741 ggaaaagaat gttattggcc ctgtagttac tacggaatat ggttatcatg ttattcaggt
     4801 tttagatcac aatccgaaaa aaacaatcgc tttggacgaa gttaagagca aaatatcggc
     4861 tcatctggaa caacaaaaaa aggcggaagc ttttgccttg attatgaaga agttacaagg
     4921 aaatgcgaag ataataattt atcaaaatta atgcttatac caattctaaa tcaaagcgct
     4981 atttttgttg
//
LOCUS       DASVIM010000062         1724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_449865, whole genome shotgun sequence.
ACCESSION   DASVIM010000062 DASVIM010000000
VERSION     DASVIM010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449865"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..814
                     /gene="rpsF"
                     /locus_tag="VF305_01290"
     CDS             323..814
                     /gene="rpsF"
                     /locus_tag="VF305_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX7415798.1"
                     /translation="MKRYEVIVIVKSDLNDEDITALIERSQTIITDRKGVIAKTDKWG
                     KRHLAYEINKQKDGYYFLIDFAGDGPIITEIERNFKIDDRILKFMTVKKEGATTRETI
                     DAEFAAAEVKRTEVRIESDTAVSSTGENTIRQIEQVSEETKTRRVLSKKPSTKEELPT
                     KGE"
     gene            818..1087
                     /gene="rpsR"
                     /locus_tag="VF305_01295"
     CDS             818..1087
                     /gene="rpsR"
                     /locus_tag="VF305_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX7415799.1"
                     /translation="MATPNGDRPTRYPQKKFFHRKKFCRFCTDSNIKIDYKNPAILNN
                     FVTERGKIMPRRITGNCASHQRELAIAIKRARTIAIMPFVTAEGR"
     gene            1191..>1724
                     /locus_tag="VF305_01300"
     CDS             1191..>1724
                     /locus_tag="VF305_01300"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEX7415800.1"
                     /translation="MKTFGILFPHNPFLRILLITSFFVAAAAFVPIFDITFLLLLPMI
                     LFFYGVTTSRIKSAGAFLISFSLLFFFSHFFQFALPYPAILTMGIAGLIISTLILKNG
                     SIEKTVIYTSLIIIASICAYFLYSGYNQNINPWHLVQKFVAATVEENIKMYSKLPFDK
                     EDINFIKDNKQSIIDFFT"
BASE COUNT          539 a          374 c          309 g          502 t
ORIGIN      
        1 tattatcttt ttcttctaat tccaattata aatcaaatcg ctatctctat tggctgagcc
       61 tgcccggcgc ataacctgaa ggtcacacgt gcctggggca tcgtcggctt ccacaaagta
      121 tgtaaaatac gcctcatcgc cttctcctcg cctcctcgat atcatctttg acttggaaat
      181 ggtataagta gtatatagca atgagcataa tataatcctt gatttctgtt ggagttcggt
      241 ataaaagtcg ccatcataaa ttttaggact ccccttgcct gtggccttga ctatgggctt
      301 tagagccgaa aggagaaaaa atttgaaaag atacgaagtt atagttatag ttaaatccga
      361 tttgaatgat gaagacatca cagcattaat cgaacgtagc cagacaatca ttaccgaccg
      421 caaaggtgtc attgccaaaa ccgacaaatg gggcaaaaga catcttgctt atgaaatcaa
      481 caaacaaaaa gacggttatt actttcttat tgactttgcc ggtgatggtc ctattatcac
      541 ggaaattgaa agaaatttca aaattgacga ccgcattctg aaattcatga cggtaaaaaa
      601 ggaaggcgcc actacgcgcg aaaccatcga tgcagaattt gctgccgctg aagtaaagcg
      661 cacggaagta cgcatagaaa gcgataccgc ggtttcttca accggagaaa atactatccg
      721 tcagatagaa caagtatctg aagagacaaa gacacgcaga gttttatcta agaaacctag
      781 cactaaagaa gaactcccaa cgaaaggcga ataaccaatg gctacaccca acggagatag
      841 accaactaga tatcctcaga aaaagttttt tcatagaaag aaattttgcc gcttttgcac
      901 ggattccaat attaagattg actataaaaa tcctgccatc ctcaacaact ttgtaaccga
      961 gcgaggcaag attatgccgc gtagaatcac cggcaattgc gctagccatc aaagagagtt
     1021 ggccatagca ataaaacgcg cccgcacaat tgcgattatg ccttttgtca ccgccgaagg
     1081 aagataaaag aaattttact ataaacaaag ctcccggcct tttcctttaa cgaaaaaggc
     1141 cggctgactt tctaattttc cgaacagtta ctttgaacag gttatcagtt ttgaaaactt
     1201 tcggcattct ctttccccat aacccctttc tcagaatttt attaattact tctttttttg
     1261 tcgcagccgc tgcttttgtt ccaattttcg acatcacttt tcttctgctg cttccgatga
     1321 ttttattttt ttacggcgta accacgagca ggattaaatc ggcaggcgca tttctgatct
     1381 ctttttcatt gctttttttc ttttctcatt ttttccaatt tgctttgcct tacccggcaa
     1441 ttttaacaat gggaattgcg ggtctgataa tttccacatt aattttaaaa aatggatcaa
     1501 ttgaaaaaac ggtaatttac acatccctga ttattatcgc gtcaatctgt gcttatttcc
     1561 tttacagcgg ttataaccaa aatataaacc cgtggcatct cgtccaaaaa tttgtcgccg
     1621 caacggttga agaaaatata aagatgtaca gtaagctgcc atttgataaa gaagacatta
     1681 attttattaa ggacaacaaa cagtccatca ttgatttctt cacc
//
LOCUS       DASVIM010000063         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1390183, whole genome shotgun sequence.
ACCESSION   DASVIM010000063 DASVIM010000000
VERSION     DASVIM010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390183"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VF305_01305"
     CDS             complement(<1..391)
                     /locus_tag="VF305_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7415801.1"
                     /translation="MTINNKDSLEPAKPKFSELLASPDVKERWEKVRKYFFLRESTYD
                     MSNRCNLRCDGCYYYEGEKQFAAENGETQAWRKLMQAEKARGITYVVLAGAEPSLVPE
                     LLEVCYQEMPLGSIATNGFKKIPESVGY"
     gene            complement(412..684)
                     /locus_tag="VF305_01310"
     CDS             complement(412..684)
                     /locus_tag="VF305_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX7415802.1"
                     /translation="MPENPDIKGIIRHVAEVIIGELKLEDVTADTFNPDMDLVDELGI
                     DSMDLATIALVLQDEYKIVIDEDDYPKLKTIRLIADYIKEKLTAKS"
     gene            complement(698..1084)
                     /locus_tag="VF305_01315"
     CDS             complement(698..1084)
                     /locus_tag="VF305_01315"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415803.1"
                     /translation="MKGQWNLLGEVKSDKPNEIEALAYVPTDSPWFVGHFPNEPILPG
                     IALIHMAEQAIIRHTEKRGEQVKLSALRRVRFTQPVKPGENLSLTISGEELGEETLFS
                     FKVANSENIVCSGLIIAQKIKKDKKV"
     gene            complement(1252..>1911)
                     /locus_tag="VF305_01320"
     CDS             complement(1252..>1911)
                     /locus_tag="VF305_01320"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7415804.1"
                     /translation="QTNFFLPVISRGSTRENLVALTFDDGPSKPTTTQILDLLDKHSM
                     KATFFVSGINAVHHPDIINDIIARGHSIGNHSYNHNPFLMLRGYDTLYQEIFTAQEVL
                     RNMGIKTRAFRPPVGIVNPKLPSILDNLGMYCLTFSCRAFDAGNYFIKNIGQKILKKV
                     KADDIILLHDVSARSKEDNTIFLSEIEILLAGIISRGFRVVPLSVLINKEVMIIENKN
                     L"
BASE COUNT          523 a          407 c          358 g          623 t
ORIGIN      
        1 tgtagccgac agattccggt atttttttaa aaccgttggt ggcgatactg cccagcggca
       61 tttcctgata acacacctct aatagttccg gtacgagtga tggttcggct ccggccaaaa
      121 caacataagt gataccgcga gctttttcgg cttgcataag tttccgccat gcttgcgttt
      181 ccccattttc cgcagcaaac tgtttttccc cttcatagta atagcatcca tcgcagcgca
      241 gattacagcg gttactcatg tcataagttg attcccgcag aaaaaaatat tttctgactt
      301 tttcccagcg ctctttcaca tccggcgagg ctaaaagctc ggaaaatttc ggtttggcag
      361 gttccagaga atctttattg ttaatagtca tagacaatta atttctatcg attaactttt
      421 cgcggtaagt ttttctttga tataatcggc tatcaggcga atagttttca attttggata
      481 atcgtcttca tcaatcacaa ttttatattc atcctgcagt actaaagcaa tagtggctaa
      541 atccatacta tcaataccca gttcatccac cagatccata tccgggttga atgtatcggc
      601 agtaacatct tctagcttaa gttctccaat tatgacttcg gccacatgac gtataatgcc
      661 ttttatatcg ggattttcag gcattcttat cctccttcta cactttttta tcttttttta
      721 tcttctgcgc aattattaaa ccactgcata cgatattttc gctatttgct actttaaagg
      781 aaaacagtgt ttcttcgccc aattcttcac cggagatggt cagcgataaa ttttctccgg
      841 gttttaccgg ttgggtaaat ctcactcttc tcagcgcgga aagcttaact tgctcgcctc
      901 ttttttcggt atggcggatg atcgcctgtt cggccatatg aatcaaagct attcccggaa
      961 gaatcggctc attggggaaa tgtcctacaa accagggtga atcagttggc acatacgcta
     1021 atgcttcaat ctcatttggt ttatcggatt taacctctcc tagcaaattc cactgacctt
     1081 tcaaaagatg atacctcgtt tactaccgtg caaaatttaa tttattttga gcaatgtttc
     1141 tttatatcac taattatttt ttaatcaaaa ttttttataa aatcaatgat taatttaccc
     1201 cgttagaaaa tagaagactt tctaacgggg tttacttttt ggaaaagatt tttataaatt
     1261 tttattttct attatcatca cttctttgtt tattaaaacg gaaagcggta caactctaaa
     1321 acccctggaa ataattccgg ccaacagaat ttctatctcc gataaaaaaa tggtattatc
     1381 ctccttgctc cgcgccgaca cgtcgtggag caagataatg tcatcggctt ttactttttt
     1441 cagtattttt tggccgatgt tttttataaa ataattgccg gcatcaaagg cgcggcagct
     1501 gaatgtcagg caatacattc ccaggttgtc gagaattgag ggaagcttgg gattgactat
     1561 accgaccggc ggccggaaag cccgcgtttt tatccccata tttcgcaaaa cttcttgagc
     1621 cgtgaatatt tcctgataaa gagtatcata acctctaagc atcaaaaatg gattatggtt
     1681 atatgagtgg ttgccaatag aatgaccacg ggcaatgata tcattaatga tatcaggatg
     1741 gtgaacagca ttgataccgg agacaaaaaa agtcgccttc atggaatgct tatcaagcag
     1801 atcaagaatt tgtgtcgtcg tcggtttgga aggaccatca tcaaaagtga gtgcaaccag
     1861 attttccctg gtactgcctc ggctgattac gggcaggaaa aagttggttt g
//
LOCUS       DASVIM010000064         4062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1304804, whole genome shotgun sequence.
ACCESSION   DASVIM010000064 DASVIM010000000
VERSION     DASVIM010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304804"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /gene="lptC"
                     /locus_tag="VF305_01325"
     CDS             complement(<1..342)
                     /gene="lptC"
                     /locus_tag="VF305_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX7415805.1"
                     /translation="MRVSKKTIIISSIIIVFVAVVIAVGAIVKIKQTKPIDLLKALPE
                     QIDLQIKGFVYTEVGEGDSQWEVKAKTATYYKKQNFALFDQVQIKLTMSDGKVYLMTA
                     DKGQMLTDKKDI"
     gene            complement(623..1165)
                     /locus_tag="VF305_01330"
     CDS             complement(623..1165)
                     /locus_tag="VF305_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX7415806.1"
                     /translation="MMKKNHKEKLKGIKLLILDVDGVLTDGRIIMDHEGHEIKHFDAR
                     DGHGLKIIQRYGIEVVLLTGRKSEVVNHRARDLGIKDVYQGLVNKKDVLEEILQKHNL
                     PASAAAFLGDDIVDIPVLKRVGFSAAVADALDVVKKSVDYVTGHKGGRGAVRELCELI
                     LQAQGKWPEIAAKYEFTEYI"
     gene            complement(1194..2015)
                     /gene="kdsA"
                     /locus_tag="VF305_01335"
     CDS             complement(1194..2015)
                     /gene="kdsA"
                     /locus_tag="VF305_01335"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX7415807.1"
                     /translation="MKKINIGDFVIGDKAGFVLIAGPCVLENEETAMDTAQYLKKLTA
                     KLKIPFIFKSSYDKANRTSIKSYRGPGAKDGLAMLKKIKEKLGVPVLSDVHRFEEINA
                     AAQVLDVVQVPAFLCRQTDFIAEIAKKARVINVKKGQFLAPWDVDNIIAKAKAAGNEN
                     IMITERGTSFGYNNLVFDVRCLPIIRDMGYPVIFDATHAVQLPGGAATASGGERDMVP
                     YLARAAVAAGVDGVFLEVHRDPEKALCDGANSLYLDSLEELLTMLKQIDSLVKSK"
     gene            complement(2022..>4062)
                     /locus_tag="VF305_01340"
     CDS             complement(2022..>4062)
                     /locus_tag="VF305_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7415808.1"
                     /translation="KISISEGLMVNRYVNTKGQNKKASHPIIRILVVFTFIILILVFL
                     YFFYPRPLHVFSPFASRVKNTFSYYILKDKPHFYYLKIEKNGKDIRVSTSEALEVTYR
                     DEFVVKAVVSDDITGEYTTVNVEGLGQGNDDLGVLLRGVDLVNKIIKRPGLIHDSGNI
                     SDYKILINYRNETIAAIPLKVEITPQDWLRFAKDSSNVKEQIEYLKKAIAMNKNDISV
                     RKILAGVYLRQDRLDEAISQYKDVLAIKHDDTTALGELAKCYIKKKEFDDAIEVSKKL
                     IKINPREAQAYVSLGLSLAEKELWDQAIQNYREAVRLESDNYPLRFRLGEAYEKKRMM
                     NLAIEEYKYIAEHSKEADMALLALGDVYLKLKKYDEAIKYYKVLIKNQPRLAAAYANL
                     ASAYAGQGNWQEELEILKKAVALSPDEPIIRFNLGAAYERRKMDDEAVKEYEHALKIN
                     PEDMDALGRIAELAFKNKKFGQAIKYYEKLAAKFPKNSSIFINLGFAYGELKKYEASA
                     ENYEKAIKQGAKSSNVHYNLAYTYGKLRKEKEAIAEYEKTFPPTKEVLSILGKFYLKE
                     KKYARAITYYKKIVILEPKKAAPYASLGYAYATSGNWDKAINNYLIALKYDREDDELY
                     ANLGEAYEKKGLYQEALKSYTSAYELNPESAKAAKKIPKLKIQLLQKKAQKKTKE"
BASE COUNT         1057 a          951 c          709 g         1345 t
ORIGIN      
        1 aatgtccttt ttatctgtaa gcatctggcc tttatccgcc gtcattaaat aaactttacc
       61 gtcagacatt gttaacttta tttgcacctg atcaaataaa gcaaagtttt gttttttgta
      121 atatgtagcg gttttagctt ttacttccca ttgggaatcg ccttcgccga cttccgtata
      181 aacgaaacct ttaatttgca aatcgatttg ctccggcaat gcttttaaca aatctatggg
      241 tttggtttgt ttaattttta ctatcgcccc aacagcaatc accacggcca caaagacgat
      301 tattattgac gatataatta ttgttttttt acttactctc attttctatt caaatccggg
      361 atgttctgtt attgcacagt acaactgaat ttactatacc attcagaatg tcgcgtgtaa
      421 agatataagg atgaagataa gttgaagaaa tgctactaat tagcaccctc tttttatcat
      481 ttataccatt ttgatttcaa aggataacgc ggcggcaagg aggaggctcc gcaggcgtat
      541 tactaacacg taaccttcag gttatacgct gaggaagcca acgatgctgc caacaaagtt
      601 agccaaagaa agcgaattgg cattaaatat attccgtaaa ctcatactta gcggcgattt
      661 ccggccattt gccctgagct tgtaaaatca gctcacaaag ttctcttact gctccgcgac
      721 cacccttatg cccggtaaca taatcaaccg attttttcac aacatcgagg gcgtcagcta
      781 ccgctgcgga gaagcccacg cgctttagca ccggtatatc gactatatca tcgcctaaaa
      841 aagctgcggc actggcaggc agattgtgtt tttgtaagat ttcttccaga acatccttct
      901 tatttaccaa tccctgataa acatctttaa ttcccaagtc acgcgcccgg tgattgacta
      961 cttctgattt ccgtcccgtc aataatacaa cttcaattcc gtaacgttga attattttca
     1021 ggccatgtcc gtcacgggca tcaaaatgct ttatctcgtg cccttcatga tccattatta
     1081 tccggccatc agtcaagact ccgtctacat ccaaaattag cagttttatt cctttgagtt
     1141 tttctttatg attttttttc atcatcgtaa ttatccttta taaattctgt tttttattta
     1201 ctctttacta aactatcaat ttgttttaac atcgtgagca gttcttctag cgagtctaga
     1261 taaagcgaat tggcaccgtc gcaaagcgcc ttttccggat cacgatgcac ttccaaaaat
     1321 acgccgtcca cgcccgcagc cacagccgcc cgcgccagat agggcaccat atcccgctcg
     1381 ccgcctgatg ccgtagcggc gcctccaggc aattgcacag catgagtggc atcaaaaatt
     1441 acggggtatc ccatatccct aataatgggc aggcagcgga catcaaaaac aaggttattg
     1501 tagccaaacg atgtacccct ttcagtaatc ataatatttt cattaccggc cgctttagcc
     1561 ttggctatta tattatccac atcccagggc gccaaaaatt gccctttctt cacattgatt
     1621 acgcgtgctt ttttcgcaat ttcggcaata aaatcggtct gccggcaaag aaaagccggc
     1681 acctgcacga catccaatac ttgcgcagca gcgttaattt cctcaaagcg atgcacatcg
     1741 gaaagcacag gcactcccaa cttctctttt atttttttta acatggctaa tccgtctttt
     1801 gctcccggac cacggtatga tttaatcgaa gtcctgttgg ctttgtcgta agaagatttg
     1861 aagataaaag gaattttcaa ttttgccgtg agttttttta gatattgagc cgtatccatg
     1921 gcagtttctt cattctccaa tacacacggg ccagcaatca aaacgaatcc cgctttatct
     1981 ccaataacaa aatcgccgat gttaattttc ttcattcttc gtcactcctt ggtttttttc
     2041 tgcgctttct tctgtaaaag ctgaatttta agttttggta tttttttggc agcttttgcc
     2101 gattccggat taagctcgta ggcgcttgtg tatgatttca gtgcttcctg atatagtcct
     2161 ttcttttcat aagcttctcc caagttagcg taaagctcat catcttcgcg gtcatattta
     2221 agcgcaatta agtaattatt aatagcctta tcccaattgc cactggtggc ataagcataa
     2281 cctaaactgg cataaggcgc agcttttttc ggctccaaaa taactatttt tttgtaatac
     2341 gtaattgccc gtgcgtactt tttttctttc aagtagaatt ttccaagaat acttaatact
     2401 tccttggttg gaggaaatgt tttttcatac tctgcaattg cctctttttc ctttcgcaat
     2461 ttaccgtatg tataggccaa attataatgc acgtttgaac ttttagcgcc ctgtttaatc
     2521 gctttttcgt aattttcggc tgatgcttca tattttttca gttcgccgta ggcaaaacct
     2581 aagttgataa aaatcgaaga atttttagga aattttgccg ccaatttttc gtagtatttt
     2641 atcgcttgac cgaatttctt gtttttaaac gctaattccg caatgcgccc cagagcatcc
     2701 atatcttcgg gattaatttt caatgcatgt tcgtattctt taaccgcttc gtcgtccatt
     2761 tttcttcttt cgtaagccgc acccagattg aagcggatta tcggctcatc aggactaagt
     2821 gcgaccgcct tttttaaaat ctccagttct tcctgccagt taccttgtcc ggcataggcc
     2881 gaagcaaggt tggcgtaagc agcggcgagc cgtggctgat tttttataag caccttataa
     2941 tatttaatag cttcgtcgta ttttttaagc tttagataga catcgcccag agcaagcaag
     3001 gccatgtccg cttccttcga gtgttcagca atatatttgt attcttcaat agctaaattc
     3061 atcatccttt tcttttcgta ggcttctccc aatctgaacc gtaacggata attatcggac
     3121 tccagccgca ccgcctcccg gtaattttgt attgcttgat cccacaattc tttttccgcc
     3181 agagaaagcc ccaggctgac ataagcttgc gcttcccgcg gattgatttt gataagcttt
     3241 tttgatacct cgatagcatc gtcaaattcc tttttcttta tatagcattt agccaattct
     3301 cccagcgccg tagtatcatc atgcttgata gccaagacat ctttatattg gcttatggct
     3361 tcatccagcc tgtcctgccg caggtaaact ccggccagaa tcttgcgcac gcttatatcg
     3421 ttcttattca tggctatcgc ttttttcaaa tattcgattt gctctttaac attcgacgaa
     3481 tctttagcaa acctaagcca gtcctgcggc gtaatttcaa ctttcagcgg aattgcggct
     3541 attgtttcgt ttcgataatt tataagaatt ttgtaatcgg atatgttccc ggaatcatga
     3601 atcaaaccag gcctttttat tattttattg acgagatcaa ctccccgcaa taatacgccc
     3661 aaatcgtcat taccctggcc taagccttca acatttaccg ttgtatattc gcccgtgatg
     3721 tcatcgctga ccaccgcttt gaccacaaat tcgtcacgat aagttacttc caaagcttca
     3781 cttgtgctta cacggatatc ctttccgttt ttctctattt tcagataata aaagtgcggc
     3841 ttgtccttca atatgtaata ggaaaaagta ttttttactc tggaagcgaa gggactgaaa
     3901 acatgtaaag gccggggata gaaaaaatat aaaaagacta aaatcaaaat aatgaaagta
     3961 aacacaacca atattcttat tatggggtgc gatgctttct tgttctgccc tttggtattt
     4021 acgtatcgat taaccattaa tccctcacta attgaaattt tt
//
LOCUS       DASVIM010000065         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_706670, whole genome shotgun sequence.
ACCESSION   DASVIM010000065 DASVIM010000000
VERSION     DASVIM010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706670"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..861)
                     /locus_tag="VF305_01345"
     CDS             complement(220..861)
                     /locus_tag="VF305_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415809.1"
                     /translation="MKKNITWESIILVCIIGFFLVGCGAASSKIKMTQGKYTPQFRAG
                     DYSRYKGKKLILAGFSNQAGNTKAWSYNSADNKYMYEGDAQLESYYWSCFRKAFRHIG
                     VNIVDYNYGGGPNPYHHGYWWGYGYAPPPEQARAAKGVPEFGLTLTSLTDKEFRFKVY
                     LYKNGETKLEKDFAVTMSAASTESVADLEKRSYRLVDLAFTTIMKDKDFQRVF"
     gene            1290..>1541
                     /locus_tag="VF305_01350"
     CDS             1290..>1541
                     /locus_tag="VF305_01350"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX7415810.1"
                     /translation="MANRIEVGFKKGIRDALGEKTKKRIIDNLGLPVEKVATIEVYTI
                     AGELSAAELQEAAAGPLSDPVIQEFSINEPLAKNFYWLIE"
BASE COUNT          454 a          328 c          308 g          451 t
ORIGIN      
        1 aaaaatagcg ccagagaagc attcctcttg aatcaacata gattaagttt taaacagcat
       61 taacgtttga ttaagattct tctagttcgg cagatgcttt atcgtcgagc aaaatatctt
      121 ctctgattta gaaatggtat agggatgtct aacaaattat ggtgtatggc gagagcggac
      181 tttttcttca tccgctctcg ccatagagta ttgattcaat taaaacactc tttgaaaatc
      241 tttatctttc ataatagtag tgaaagccaa atccaccagt cgataagaac gtttctccag
      301 atcggcaaca ctttccgtcg atgctgcact cattgttact gcaaaatctt tctctaattt
      361 tgtttcgcca tttttataca ggtatacttt aaagcggaat tccttatcgg taagagatgt
      421 cagggtcagc ccgaattcgg gcacgccttt ggcggctcgt gcctgctccg gaggaggcgc
      481 atagccataa ccccaccagt aaccatggtg ataaggattc ggaccgccgc catagttata
      541 atcaacaata tttacgccga tatggcggaa agctttacga aagcaagacc agtaatagct
      601 ctccaattgg gcatcgccct catacatata tttattgtcg gcactgttat agctccaagc
      661 ctttgtgttt ccggcctggt tgctaaagcc ggcgagaatc aacttcttcc ctttataacg
      721 gctgtaatcg ccggcgcgaa attgcggcgt atatttgccc tgggtcatct ttatcttgct
      781 gctggcggcg ccgcatccca caagaaaaaa accgataatg caaacaagga ttatactttc
      841 ccacgtaata ttttttttca taacttagat ccctcctttt aaaagtaata tcgcaaagcg
      901 aacaacctaa ctaataaatg acgactgaat ataacggcta aaagattgaa gtgtcaagta
      961 aaatgactgg cgctttatta gcttatttta ttacctaact tatttaaaaa ttaaaaaaat
     1021 ctgaatcaat ttcagaacat atttggaagt tgctttattg cgtaattttc ggtatatacg
     1081 cgccgtctgc aatctattaa gacagacaat attttaatac atttgcaaag actgtgttgg
     1141 tcattgcgaa tccgccggcg gcggatgtgg caatccctat aaatgccaaa gcctttatga
     1201 gattgcttcg gtcgtttcac tccctcgcaa aaacaattta accaatcctc gggattacag
     1261 ctttttttca aaaaatatta aggtaaaaca tggcgaatag aattgaagtt ggcttcaaaa
     1321 aaggtattcg cgacgcgttg ggtgaaaaga ccaaaaagcg cattatagac aatctggggt
     1381 taccggtcga aaaagttgcc acgattgaag tttatacaat tgccggagaa ctctctgctg
     1441 ccgaattgca ggaagctgcc gcagggccac tctccgatcc ggtgattcag gaattctcca
     1501 ttaatgagcc gctggcgaag aatttttact ggcttattga g
//
LOCUS       DASVIM010000066         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1049881, whole genome shotgun sequence.
ACCESSION   DASVIM010000066 DASVIM010000000
VERSION     DASVIM010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049881"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VF305_01355"
     CDS             complement(<1..596)
                     /locus_tag="VF305_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7415811.1"
                     /translation="MHYTVFNTPVIKLFLRGLSLFFLKILGWKTVGQLPEESKYVIIF
                     APHTSNWDVLYGVFVAFTLKLDACYLAKKELFQWPFGPLMKWLGSIPIDRTTSGHTVN
                     QMIQKFNESKRFVLAIAPEGTRRKVEYWKSGFYHIAEGAHVPLLLGFLDYVSKEGGAG
                     PRIHLTSDIYHDMQTIQDFYVTIKGKNPDQTSPAAIFL"
     gene            996..>1734
                     /locus_tag="VF305_01360"
     CDS             996..>1734
                     /locus_tag="VF305_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX7415812.1"
                     /translation="MDLPVNIVIIFILFLFSGFFSSAEAALLSLTDLHLHKMKLDRYP
                     FLTFVGKLLENPRRLLITIVTSNEAVNISISILAASFFIALLGANGQLVSIFFTTVVL
                     LIVGEAIPKTFGVTYPMQVSAMVSPLMIAISRLEYPAVAALEKLSGFFLKKYGKHTTL
                     DADALMEDEFKTLIDAGSREGVVDESQKELIKRIFALGDKPVTDIMIPRVDIFCLPLN
                     MPVEEIISEVVRKRQERVPIYTDDRDDI"
BASE COUNT          500 a          340 c          356 g          538 t
ORIGIN      
        1 ttaagaaaaa tagcagcggg gcttgtttga tcgggattct tccccttaat agttacatag
       61 aaatcctgaa tagtttgcat gtcgtggtat atatcactgg ttagatgtat gcgcggtccg
      121 gctccgccct ctttgcttac gtaatcaaga aatccgagca gtaggggaac atgggcgcct
      181 tccgctatgt gataaaaacc tgatttccag tattcaactt ttcgtcgggt accttcaggg
      241 gcaatagcca ggacaaatct tttgctctcg ttaaattttt gtatcatctg gtttactgta
      301 tgaccggacg tagtcctgtc gatggggatg ctacccagcc atttcataag cggcccaaaa
      361 ggccattgaa ataactcctt ttttgccaga taacaggcat ccaatttcag agtgaaagcc
      421 acgaaaacac catataatac atcccagttg gacgtatgag gtgcaaaaat aatgacatat
      481 tttgattctt cgggcaactg tccaactgtt ttccagccga gtatctttaa aaagaatagg
      541 gataaaccac gcaaaaataa tttaataacc ggtgtattaa aaacagtgta atgcatgaca
      601 ttggcctccg ccaataagaa tatatcgaat caaactacta ttaattttag tagtaaatat
      661 ttaccataaa acctttatca aataaacaac tgtatgttta tatttttctt tcgattcagc
      721 gcgatgagct ctcgtcgcat ctccaatgca tatacaagga acttcctctt acgggattgg
      781 gaaagataga caagaaagtc ctaagggcag aggttttttg ctaggaattt ctttgggtgg
      841 gagaaactat aagaaaaatg accttttgtg tcgattaata aataaccata aaatgtagta
      901 tgaggtggta atgctcgagg aagggatata tctttttttg cctcaatctg ctagattaca
      961 tactataaat aaatataaat gaggttgttc aataattgga tttaccggta aatatagtaa
     1021 taatatttat tctttttttg ttttccggtt ttttttcatc ggcagaagcc gcattgcttt
     1081 cgcttactga tttgcatctg cataagatga aattggaccg ctatcccttt ttgacctttg
     1141 tcggcaagtt gcttgaaaat ccccgccgcc ttttaattac gattgttacc agtaatgaag
     1201 ctgttaatat cagcatctcc attttggcgg catctttttt tatcgcttta ttgggcgcaa
     1261 acggccaatt ggtctccata ttttttacaa ctgttgtgct attgattgtc ggcgaggcta
     1321 tccctaagac ttttggcgta acttatccga tgcaagtttc ggccatggtt tctcctttga
     1381 tgatcgctat ttcccgtctg gaatatcctg ctgttgccgc attggaaaaa ttatccggct
     1441 tttttttaaa aaaatacggc aaacatacaa cccttgatgc cgacgcttta atggaagatg
     1501 aattcaagac cctgatagat gccggaagcc gtgaaggcgt agtggacgaa tctcaaaaag
     1561 aactgatcaa aagaatattc gcattaggcg ataaaccggt caccgacatt atgatccccc
     1621 gcgtggatat tttttgtctg cccctgaaca tgcctgtgga ggaaattata tcggaggtcg
     1681 ttaggaaaag gcaggaaagg gtgcctatct acacggatga ccgcgacgat atta
//
LOCUS       DASVIM010000067         5322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1220914, whole genome shotgun sequence.
ACCESSION   DASVIM010000067 DASVIM010000000
VERSION     DASVIM010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220914"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /locus_tag="VF305_01365"
     CDS             complement(<1..2157)
                     /locus_tag="VF305_01365"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX7415813.1"
                     /translation="MKEKMKLAAQVQDEEYYSGRIYEKQDFFRYQLAKIFLSRVSIAD
                     EYVETIKKLSTEGLVVYAIKQRSKLNSLIVYELAGRKGLPRPVYCHGMNMTFWQPLPE
                     MLKFFWSSFLRRFGKKMKAWLGKLAYLERIVSEKKSIVIHLGESEFIESRSADGAIIS
                     LINVQKKVDFPIFVVPVLVAYGRRREKEDESLINILFGQAEHTGALRRLITFMRYSSD
                     AFVIPAEPIKLSEYLNKSNDTPREGFVHELRGELIDRIDREKTTVVGPVLKSREELMG
                     IVLGDEKVKKFINDYAAREKKDAAAVEKEARRYLDEIAADYNETFIQLWLKALAWLWN
                     TIYDGLVIDQEGLAKIRNISKKMPFVVIPCHRSHTDYLLFSYVLYKNNLQLPFIAAGN
                     NLSFFPLGYIFRKSTAFFLRRGFRGNEVYAEVLAKYIAILLREGLPIEFFIEGGRSRT
                     GKMVMPKYGLLSMVIQAYQEKYCDNFAAIPVYIGYDRVIEEKSYLEELTGASKSPDNT
                     SEIIKTGKILRKRYGRVYLNIGEPIIMKAYLEAQEKQITEMTLDERQSLYRRIGYEIV
                     LEINKVSVVTPFSLVATVLLSHDRRGISHEELMDVLNEFYEYLSIKKVKFAATFAYRK
                     KAIADALNIFVQSGIISKIETDEDEVEEMQEVVYSLEEEKRLNLEYYKNNILHFFLPL
                     CFVATSMVNSNEDLMSLGRIIEDYKFLKKLLWNEFIF"
     gene            complement(2263..4044)
                     /gene="ptsP"
                     /locus_tag="VF305_01370"
     CDS             complement(2263..4044)
                     /gene="ptsP"
                     /locus_tag="VF305_01370"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX7415814.1"
                     /translation="MTTDQTKKTFVLKGIGVSPGVVIGRAYRFDPLDSQLSFYKLNDS
                     SLIPKEIQRFKKALKESELQLLKVQENLKKTKIAGPLYIIDVHILILRDKKFVNSTIK
                     YIRRLEINAEWAVRLTLDRYEQIFEGLEDSYIRGRISDVQYVSQRVLRNLSGEKKEPI
                     WGISEEIIIIAKDISPADTAQMKLEKVIGFATDTGGRTSHTAIVARDMELPAVVGLDN
                     VTRVVRTGDEIIVDGISGVVVVNPYPEMLKRYEEKKLLYSAARDEYLKYAKLPAVTTD
                     NYTVQIGGNIEFIEEIPSAIVHGAEYIGLYRTEFIYISRQDLPTEEDHFNNYRQVVGA
                     KDLLWSTIRTFDLGGDKFSSNQKPVKELNPQMGLRAIRFCLKEVEIFKTQLRAIWRAG
                     ALGKTKVLFPMISSVEEIREAKKLLEEARQELLTQGVKIASEMEIGAMIEVPAAVEIA
                     DKLAQEVDFFSIGTNDLIQYSLAIDRANERLTYLYEPLHPAVLRLIKRVVDIAHEANI
                     RVAMCGEMAGDPLCALILLGMELDELSMNHLAIPRIKRIIRESSVKESKILLEKALTF
                     STASEVRAYVQDYMMGRFPGEFQNEEA"
     gene            complement(4053..4322)
                     /locus_tag="VF305_01375"
     CDS             complement(4053..4322)
                     /locus_tag="VF305_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX7415815.1"
                     /translation="MTEIRTLTLKNKLGMHARAAAAFVKVAQQFKAEIYLERNGQIAN
                     GKSILGILTLDCPMGGMLTIKAEGVDALKAIEALEILIENKFGEV"
     gene            complement(4319..5071)
                     /locus_tag="VF305_01380"
     CDS             complement(4319..5071)
                     /locus_tag="VF305_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEX7415816.1"
                     /translation="MKKTTLMKIFLRSFFIQTTLNFRRMQNLGFTMAIIPLIREWRLQ
                     QKDAEGMLTRHLQMFNTHPYFSAPVIGSVVRLEEEQGGKGDALEIIALKQSLMGSYAA
                     IGDIFFWGALRPFAAIIAVILAYMGLIIAPIAFLLIYTPAHFWIRLKGFIEGYRLGKN
                     GFEFIRTMDLPRVAVKIRWLSLIILTGSIIWLSYDGYWPFMNTPAVIMKLVALASVLL
                     CLLLIKQGVSQIYIIYGAVVMFLLISWRGILN"
BASE COUNT         1546 a         1220 c          981 g         1575 t
ORIGIN      
        1 aaaaataaat tcattccaca aaagtttttt gagaaattta taatcttcga ttatccgccc
       61 cagtgacatc aaatcttcat tgctattaac catggaagta gcaacaaaac acaaaggcaa
      121 aaagaaatgc aaaatattgt ttttataata ctccaaattg agcctttttt cttcttccaa
      181 cgaataaacc acttcctgca tttcctcgac ttcgtcttca tcggtctcaa tttttgagat
      241 aattcccgac tgcacaaaaa tattgagggc atcggctatc gcttttttcc gataggcaaa
      301 agttgcagca aacttaactt tttttatgga tagatattca tagaattcat ttaaaacatc
      361 cattaattct tcatgagata ttcctcggcg atcatggctc aaaagcactg ttgccacaag
      421 ggagaatggt gtgactacag agaccttatt aatttccaga actatttcgt agccgattct
      481 tcgataaagg ctttgccgtt catctaaagt catctcggtg atttgttttt cttgcgcctc
      541 taaataggcc ttcataataa tcggctcgcc gatattcaaa taaacacggc catagcgctt
      601 gcgcaatatt ttgccggtct taattatctc ggaggtgtta tccggagact tggacgctcc
      661 ggtcaattcc tccaggtatg acttttcttc gatcacgcgg tcgtaaccaa tataaacggg
      721 aatcgcggca aaattatcac aatatttttc ctggtaggcc tgaataacca tggaaagaag
      781 accatatttg ggcattacca tttttccagt ccgactacgg cctccttcga taaaaaattc
      841 tatcggcaac ccttcccgca gcagtatagc aatatacttt gccagcactt cggcataaac
      901 ttcgttgccc ctgaagccgc gccgcagaaa aaaagcggtg gacttgcgaa aaatgtaacc
      961 cagcgggaaa aaggataaat tgttgcccgc ggcaataaaa ggcagttgaa gattattctt
     1021 gtataaaacg taagaaaata aaagataatc agtatgtgaa cgatggcagg gaataaccac
     1081 aaaaggcatc tttttggaaa tatttcttat tttggccaag ccttcctgat caatcaccag
     1141 gccgtcgtaa atagtgttcc acagccaggc aagggccttt agccaaagct gaataaatgt
     1201 ttcattgtaa tcggcggcga tttcatcaag ataccggcgc gcttctttct ccaccgctgc
     1261 cgcgtccttt ttttcccgcg ctgcataatc attgataaat tttttcactt tttcgtcgcc
     1321 gagaacaata cccatgagtt cttcccgcga cttcaaaaca ggtccaacca cggttgtttt
     1381 ctccctgtcg atacggtcaa tcaactcgcc gcgtaattcg tgaacaaacc cttcccgggg
     1441 agtatcatta cttttattta aatattcgga taatttaatc ggttccgcgg ggataacaaa
     1501 agcatcgctg gaatagcgca taaaagttat taaacgacgt aaagctcctg tatgctctgc
     1561 ttgtccaaaa agaatgttaa tcaggctttc gtcctctttt tcacgcctgc gcccgtaagc
     1621 gaccaacacc ggcacgacaa aaataggaaa atcaaccttt ttttgtacat taatcagact
     1681 gatgatcgcg ccatctgccg atctgctttc aatgaattcc gattcaccca aatgaataac
     1741 aatgctcttt ttttcactaa ctattctctc gagataagcg agcttgccca accaagcctt
     1801 cattttcttc ccaaaacggc gcaaaaaaga agaccagaaa aacttgagca tttccggcag
     1861 cggctgccaa aaagtcatgt tcatcccgtg gcaatacacc ggtcgcggaa gcccctttct
     1921 cccggcaagt tcataaacga tgaggctgtt gagtttgctc ctttgcttta tagcgtaaac
     1981 cacaagccct tcagtggaga gttttttaat ggtctcgaca tattcatcgg caatggaaac
     2041 acgtgaaagg aatatttttg ccaattggta ccgaaaaaaa tcctgctttt cgtaaatacg
     2101 tccggagtaa tattcttcat cttgaacttg tgcagctaat ttcatctttt ctttcatctc
     2161 tgtggttttt cctggaaaag gccaagtctc attgaattta aaaacctgcg ggtgacagca
     2221 ttgatttttc gcaggtttga tctagtggtt tttcaacaaa agctaagctt cttcattttg
     2281 aaattcgcca gggaaacgtc ccatcatata atcctgcaca tacgcccgca cttcggaagc
     2341 tgtgctgaag gtaagagctt tttccagcaa tattttagat tccttaacgg atgattccct
     2401 tattattcgt ttaatccgcg gaatggccaa atggttcata cttaattcat ctaattccat
     2461 ccccagaaga atcaacgcgc atagaggatc gccggccatt tcaccgcaca tagccacacg
     2521 aatatttgct tcatgagcta tgtctactac tctctttatc aatctcaaaa cagccggatg
     2581 caaaggttca tacaaatatg ttaaacgttc attggcccgg tcaatagcta aagaatactg
     2641 aatcaaatcg tttgtgccga tgctgaaaaa atctacctcc tgcgccagtt tatcggcaat
     2701 ctcaactgcg gcgggcactt cgatcatcgc ccctatttcc atttccgacg caattttcac
     2761 gccttgcgtc agaagttctt gacgggcttc ttccagcaat ttttttgctt cgcgaatttc
     2821 ctctacgctg gaaatcatcg ggaaaagaac tttggttttt cctaatgcac cggcacgcca
     2881 aatggctctt agttgcgtct tgaatatttc aacttccttc agacaaaaac gaatcgccct
     2941 caatcccatt tgtggattta gttccttgac cggcttttga ttagaagaaa atttatcacc
     3001 gcctaaatca aaagttcgaa ttgttgacca aagtaaatct ttagcgccga cgacctggcg
     3061 gtaattatta aaatggtctt cttccgttgg taaatcctgg cgactaatgt atataaactc
     3121 cgtccggtaa agcccgatgt actctgcgcc atgcacaatt gccgaaggta tttcttcaat
     3181 aaactcgata ttgcccccta tctgcactgt atagttatcg gtggtaactg caggtaattt
     3241 tgcatattta aggtattcgt ccctggcggc tgaatagagg agcttttttt cttcgtatct
     3301 ttttagcatt tccgggtagg gattaacaac gaccaccccg gaaataccat ccacgattat
     3361 ttcatctcct gtgcgtacaa ctcgcgtgac attgtccaag ccgactacag caggcaattc
     3421 catatcgcgg gcaacaatag ctgtgtgcga agtgcggccg ccggtatcgg tggcaaaacc
     3481 aataactttt tccaatttca tttgcgctgt gtccgcagga gaaatatcct tcgcaataat
     3541 gattatctcc tcgctaattc cccagatagg ttcttttttt tcaccggaaa ggttgcgtaa
     3601 aactctttgg ctgacatact ggacatcact gatacggccc cggatataag aatcttctaa
     3661 tccttcaaat atttgttcat aacggtcaag agtcaaacgc accgcccatt cggcgttgat
     3721 ttctaatctg cgtatatatt taattgtact gttgacaaat tttttgtccc ttaaaatcaa
     3781 aatatgaacg tctattatat aaagaggtcc ggcaattttt gtttttttaa ggttttcttg
     3841 aacttttaat agttgaagct ctgactcttt cagggctttt ttaaagcgct gtatttcttt
     3901 gggaatcaac gaagaatcgt taagtttata aaaagaaagc tggctgtcta gtggatcaaa
     3961 tcggtaagcc ctgccgataa caacacctgg agaaacaccg atgcctttaa gaacaaatgt
     4021 tttctttgtt tgatcagttg tcattttatc gcttaaactt ccccaaattt attttcgatc
     4081 aggatttcca gcgcttctat agccttcaat gcgtcaacac cctcggcttt tatcgttagc
     4141 attccgccca ttggacaatc cagagtaagt atacccagga tacttttacc gttagcaatt
     4201 tgcccgttgc gttcgagata aatttccgct ttaaactgtt gagctacttt tacaaaagct
     4261 gccgctgccc gcgcatgcat tcccaattta ttttttaatg ttagggttct aatttccgtc
     4321 aattcaaaat tcctctccag gaaataagaa gaaacattac aacagcgcca taaataatat
     4381 aaatttgcga cacaccttgt ttaatcaata aaagacaaag caaaacagat gccagtgcca
     4441 ctaatttcat aataacagcg ggagtattca taaaaggcca atatccatca tacgacagcc
     4501 aaataatcga acccgttagt ataattaaag atagccagcg aatttttacg gcaacacgag
     4561 gcaaatccat agtgcggata aattcaaagc catttttccc cagccggtaa ccttcgataa
     4621 aaccctttaa tcttatccaa aagtgagccg gtgtataaat taataagaaa gcaataggag
     4681 caatgattaa ccccatatag gccaaaataa cagcaataat cgcggcaaaa ggcctcaggg
     4741 cgccccaaaa aaaaatatcg cctatagcgg cataagaccc catcaagctt tgtttaagcg
     4801 caataatctc taaagcgtca cctttaccgc cctgttcttc ttccaaacgg acaacggagc
     4861 cgatcaccgg cgcagaaaaa tagggatggg tgttaaacat ctgcaaatgt ctggtcagca
     4921 ttccttctgc atccttttgt tgtaaccgcc attcccgaat aagaggaatt atcgccatgg
     4981 taaaacctaa gttttgcatt cgccgaaaat tgagtgttgt ttggataaaa aaagacctca
     5041 aaaaaatttt cattaaagtc gtttttttca cttttttgcg gcctcaaaag taattagctg
     5101 catattttta gtgtctttta ttgcagtgcg tgtcactaac ataaaaggct ggcgaagtca
     5161 atttaatcga ggaataacgt taaatatgcg tgattgttcg actaaaaaat ttcaacgtgt
     5221 aacctcgcgt gccctttagg gtattaggtt atgcacttcg tttttgaaac ttgagtctca
     5281 cgaataaaga gaggactcgc agccagggaa aacaaaataa tc
//
LOCUS       DASVIM010000068         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_368174, whole genome shotgun sequence.
ACCESSION   DASVIM010000068 DASVIM010000000
VERSION     DASVIM010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368174"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VF305_01385"
     CDS             complement(<1..747)
                     /locus_tag="VF305_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7415817.1"
                     /translation="MEKISFTGLLKSQKEFFWSGKTRDLNFRIEALTKLKNIIKAQEE
                     EVLNAIYEDLHKVSLDGYATEISGVYDEIKYALENVRSWAKTEKVETPVFLLPSKSYV
                     QKDPYGVTLIIAPWNYPFQLLMTPLVGAIAAGNTAILKPSEISVSTVAVMEKIINQAF
                     PKEYLHVVQGGAVETQALLDLPVDYIFYTGSAAVGKIVMSAAAKNLTPLTLELGGKSP
                     AIVHEDANLTHAARKIAWGKFINAGQTCIAP"
     gene            1155..1565
                     /locus_tag="VF305_01390"
     CDS             1155..1565
                     /locus_tag="VF305_01390"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEX7415818.1"
                     /translation="MIGVLITTHGNLGNELIKAAELIKGPLNDISHVSLDQTKDVEEI
                     KKEIGNAIKKLDKGRGVLILTDLFGGTPSNISLSFMKEGKVEVLTGVNLPMLLKLSDI
                     KEDMSLREFACFIKEYGQKNISLASEILSKKALG"
     gene            1570..2082
                     /locus_tag="VF305_01395"
     CDS             1570..2082
                     /locus_tag="VF305_01395"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEX7415819.1"
                     /translation="MAQDLNIALVRVDNRLVHGQILEAWVPYIKAKCIIVVDNNISGD
                     VFSETVIRMAVPSEIELIISSVDEFAKNYIFAQDGGKKTIVLFSNIADVCKAFNLGFH
                     FDKLNIGNIYNDEYKICCSPSVFLCDKEIQNILTLLEKAGVVIELKRVPRERAVNIRE
                     ALKDYCNIKP"
     gene            2098..2793
                     /locus_tag="VF305_01400"
     CDS             2098..2793
                     /locus_tag="VF305_01400"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEX7415820.1"
                     /translation="MFTKIILLSFCGGLLCLDRVFIQAMISRPVVIAPLIGLLLGNPY
                     AGLVIGAIVELIWIDHIPVGSYLPPNDSIVAVVATSTAVIAGQKLGGVFPELIALSLL
                     MSIPCGILAKRMDSLIIKSNDSLSDQALEDAKKSNIRAIEKKVYSGLIKVFSFDVFYL
                     LVIQATFIPLVIWVYPKLPATIISTLSFTYYFLPLLGIAVAINSIKLRGAIPVFCAIF
                     LIVAVFLEFFHVL"
     gene            2783..3262
                     /gene="rimI"
                     /locus_tag="VF305_01405"
     CDS             2783..3262
                     /gene="rimI"
                     /locus_tag="VF305_01405"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX7415821.1"
                     /translation="MSYEKAALQVTIDLMGKTDLPEILAIEKESFPLPWTTGMFINEL
                     NTAHAQCLSARINYNGKSIVCAYIIFWIVADEAHLHNLAVKKEYRRQGLAFNLLGVMK
                     EIAKQTGLKAQTLEVRPSNTAAIKLYDKCGFVVKGIRPLYYPETHEDALIMWADVTR"
BASE COUNT          991 a          611 c          738 g         1027 t
ORIGIN      
        1 cggagctata caggtttgtc cggcattgat gaattttccc caggctattt tacgcgccgc
       61 gtgggtaaga ttggcgtctt catgcacaat cgccgggctt ttgccgccca actccagtgt
      121 gaggggagtc aaatttttgg ctgccgcgct catcacgatt ttacctacgg cagcgctgcc
      181 ggtatagaat atgtaatcta caggtaaatc caaaagcgcc tgcgtttcca ctgcgccgcc
      241 ctgcactaca tgcaaatatt ctttaggaaa agcttgatta atgatttttt ccattactgc
      301 caccgtgctt actgaaattt ccgatggttt aagaatcgcc gtgtttccgg ccgcaatcgc
      361 accgaccagc ggagtcataa gaagctgaaa cggataattc cagggagcta ttataagagt
      421 tacaccgtaa ggatcttttt gcacgtaact tttagaaggc aaaagaaaaa ccggagtttc
      481 gactttttcg gtcttagccc aggaacgaac attttccagg gcatatttga tctcgtcata
      541 tacgccggaa atctccgtgg cataaccatc taaggagact ttatggaggt cttcatatat
      601 ggcgtttaac acttcttctt cctgcgcctt gataatgttt ttaagtttag ttagtgcttc
      661 gatgcggaag ttaaggtcac gcgtctttcc cgaccagaag aattcttttt gtgattttaa
      721 tagcccggtg aaggagattt tttccatttt agccttcctt atggttatta cgttggcgga
      781 ttgcttttgg gcaatatatg ctggagtcat tttttagtca agttgtgagc aataagcggt
      841 taaacattct gatggcaatt ttttgttgat aaagagaaaa aatgttatat atgggcgagt
      901 gaggatttat tggccggggc gattttaaga ggcatttccc gcgggtccgg cctggctgac
      961 gctttgcgaa ataaaaaaca tgaaaaagtt tgataatatg gatttattac aataacttac
     1021 aagcctgcga gcatttcatt tttcccttta aatagtccgt ctataaacct tgacaattat
     1081 aatgcaatcc attagcttac cgcattaaat ttaagagcct ggctgaaaag agcttaaagg
     1141 gggataatat aataatgatc ggtgtgctta ttactacgca tggaaacctg ggcaatgagt
     1201 tgattaaagc agcggagtta attaaagggc cgttaaatga tatatcgcat gttagtttag
     1261 atcagacaaa agatgttgaa gagataaaaa aagaaatcgg caatgccata aagaaactgg
     1321 acaagggcag aggagtgttg attcttacag acctttttgg cgggacgcct tcaaatattt
     1381 cgctttcctt tatgaaagaa gggaaagttg aagttcttac cggagttaat ttaccaatgt
     1441 tgctcaaatt gtcggatata aaagaagaca tgtctttgcg ggaatttgcc tgctttatta
     1501 aagaatatgg gcaaaaaaat atttccctgg ctagtgaaat tttaagcaaa aaagctctcg
     1561 ggtaatcaat tggctcaaga tctgaatata gccctggttc gggttgacaa caggcttgtc
     1621 cacggtcaaa tactggaggc atgggtgccg tatattaagg caaagtgcat tatagttgta
     1681 gataacaata tatccgggga tgtctttagt gaaaccgtga tcaggatggc cgtgcccagt
     1741 gaaatcgaat taattatcag ctctgttgat gaatttgcca aaaattatat tttcgctcag
     1801 gatggcggga aaaaaacaat tgttttattt agtaacattg ccgatgtatg caaagctttt
     1861 aatttgggtt tccattttga taagttaaat atcggcaata tttataatga tgaatacaag
     1921 atttgttgct ccccttccgt ctttttatgt gataaagaaa ttcagaatat tctaacgctg
     1981 ttggaaaaag ccggggttgt tattgaatta aaacgagtac cgcgggaaag ggctgttaat
     2041 attagagaag cacttaaaga ttactgcaat ataaagccat aaaatattgg tatttaattg
     2101 tttacaaaaa ttattttatt atctttttgc ggcggcctgc tatgtctcga tcgcgttttt
     2161 attcaggcca tgatttcccg gccggtggta attgctccgc ttatcggact tcttttgggt
     2221 aatccttatg caggattggt aattggagcg attgttgaac tcatttggat tgaccacata
     2281 cccgtaggaa gttatcttcc tcccaatgat tccatagtcg ccgtagtggc aacttcaact
     2341 gctgtaatag ccggtcaaaa attgggaggc gtattcccgg aattaatcgc gctttctcta
     2401 ttaatgtcca ttccttgtgg aattttggcc aagagaatgg atagcctgat tattaaatcc
     2461 aacgacagtc tttccgatca agcgctggaa gacgcgaaaa aaagcaatat tcgcgcgata
     2521 gaaaaaaaag tttattcagg attaattaaa gtattttcat ttgatgtctt ttatctttta
     2581 gtaattcagg caacctttat tcctttagta atctgggtgt atccgaaatt accagcaacg
     2641 attatatcaa cgctttcctt tacatattat tttttaccgc tgttgggcat tgccgtggcc
     2701 attaattcga ttaaactgcg gggagccatc cccgtttttt gcgcgatatt cttaatcgtg
     2761 gccgtatttt tggaattttt ccatgtctta tgaaaaagct gcccttcagg tgacaattga
     2821 tctgatgggg aagacggact tgcccgaaat attggcaatt gaaaaagaat catttccatt
     2881 accatggacg acgggtatgt ttataaatga acttaatacc gcgcatgccc aatgtctttc
     2941 agcacggata aattataacg gaaaaagtat cgtatgtgcg tatattattt tttggatagt
     3001 tgccgatgaa gctcatttgc acaacctcgc agttaaaaaa gagtatagaa ggcaaggtct
     3061 tgcttttaat ctcttaggag taatgaaaga aatagcaaaa caaactggac ttaaggccca
     3121 gactttagag gtcaggccat ccaatacagc agcaataaaa ctttacgata aatgtggttt
     3181 tgtagtaaaa ggaataagac ctctttatta cccggaaaca catgaagatg ccttgattat
     3241 gtgggcggac gtgacccgtt agagaagcgg gtcaaataca aagaatgtgc aatcctaaat
     3301 ggagaaatga aatcaacacg acgcggaatc ggcataatcc tctaacgggg taaagataaa
     3361 aaatggc
//
LOCUS       DASVIM010000069         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_197829, whole genome shotgun sequence.
ACCESSION   DASVIM010000069 DASVIM010000000
VERSION     DASVIM010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197829"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1867)
                     /gene="pheT"
                     /locus_tag="VF305_01410"
     CDS             complement(<1..>1867)
                     /gene="pheT"
                     /locus_tag="VF305_01410"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7415822.1"
                     /translation="GDTVLDIGITPNRADCLSMIGIAREVAAITGKKMKIPAEKVKES
                     AEDINLLTSVRILDPQICPRYTARLINNVKIGPSPIWMKTRLEAAGLRAINNVVDVTN
                     FVMLEMGQPLHAFDFRFLEKGRIIVRKSKEDEEFLSLDGKSHKLSDDTLLICDGVKPV
                     AIGGIMGGLNSEVNDDTQIVLLESAYFNPTSIRRTARKLSMPTDASFRFERGIDPEGV
                     LRALNRAAQLIADISGGVICKNYIDEYPQKIPAIKDITLRLSRIREVIGTPIGGKDVV
                     RILRSIGMLVSEAGKGMYIVTPPTFRVDITREIDLIEEVARLYGYDRIPVTLPNIAIT
                     EIAAVPRMDLEERIRQLLIGSGYSEVVNYSFITPVSADYLCFPENDERRKLVRINNPL
                     VEDQSVMRTTIIFGLLDTLKKNINNGIFDLKIFEMGRVFFSRGLGKLPDEKNMLAGLL
                     AGAMTDDLCGSNTTADFYNIKGCLENILFDLKLNDHRYISANVEPFLHPGKSCGIFLD
                     DVQIGFLGEIHLDVLEKMSLKNAPYVFEINLDLLVAAYLNKCIIYEAISKFPPVMRDV
                     AFVIPEAIEADQMLNVVLGGKEDLLENVVIFDIYNGKGIPEGTKSLGLRFSYRAPD"
BASE COUNT          550 a          438 c          345 g          534 t
ORIGIN      
        1 gatcaggagc acgataagaa aaccgcagcc ccaaactttt cgtcccttct ggaatacctt
       61 tgccattata tatatcaaaa ataaccacat tttcaagcaa atcttctttt ccccctaaaa
      121 caacgttcaa catttgatcg gcttcgatcg cctcaggaat aacaaaggct acatcacgca
      181 tcactggcgg aaactttgat attgcctcgt agataatgca tttgttaaga taagcagcaa
      241 ccaaaagatc taaattaatt tcaaaaacat aaggtgcatt ttttaaactc attttctcca
      301 aaacgtcaag atgtatttcg cctaggaaac cgatttgtac atcatctaaa aaaatgccgc
      361 aggatttgcc tggatgcaaa aaaggctcaa catttgccga tatatatcga tgatcgttta
      421 attttaaatc gaaaagaata ttttcaaggc aacctttaat gttataaaaa tcagcagttg
      481 tattcgagcc acacaagtca tctgtcatcg ctccggccag gagccctgcc agcatatttt
      541 tttcatcggg aagcttacct aatccgcggc taaaaaaaac ccggcccatt tcaaatattt
      601 ttaaatcgaa aatgccgtta ttaatgtttt tcttcaacgt atccagcaga ccaaaaataa
      661 tcgttgtccg cattacagac tggtcttcta ccagtggatt attgatcctc accaacttcc
      721 gtctttcgtc attttccgga aaacacaggt agtcggcaga aactggtgtg atgaagctgt
      781 aattgacaac ttcagaataa ccactgccaa taagcaattg cctgattctt tcttctaaat
      841 ccatccgcgg aacagctgct atttctgtaa tagcaatatt gggaagcgtc accggtatac
      901 gatcatatcc ataaagtcgg gctacttctt caatcaagtc aatttctctg gttatgtcca
      961 cgcgaaatgt cggcggtgta acaatataca tacctttacc ggcttcactt acaagcatgc
     1021 cgatactgcg taatattcga acaacgtctt tccctccgat aggcgtgccg attacttccc
     1081 gaattctgct gaggcgcaag gtaatatctt taatagcggg aatcttttgc gggtattcgt
     1141 cgatataatt tttacaaata acgccgccgg aaatatcagc aatgagctgc gcggcgcggt
     1201 tgagcgctct gaggactccc tccggatcga tgcctctttc aaagcgaaaa gaagcatcgg
     1261 ttggcattga tagttttcgt gctgtgcgcc gaatagaagt gggattaaaa taagcacttt
     1321 ccaataatac aatctgtgta tcatcgttaa cctcggagtt gaggccgccc atgattccac
     1381 cgatagccac gggctttaca ccgtcacaaa tcaataaagt atcatcagaa agcttgtggc
     1441 ttttgccatc gagcgaaagg aattcttcat cttctttgga cttgcggaca atgatccggc
     1501 ctttttccaa aaaacgaaag tcaaaagcgt gcaggggctg tcccatctcc aacataacga
     1561 aattcgttac atcaacaaca ttatttattg cccgcagtcc tgctgcctct aatcttgttt
     1621 tcatccatat cggcgaaggt ccaattttta cattgttaat cagccgcgcc gtgtaacgcg
     1681 ggcaaatttg cggatcaaga attctcactg aagttaataa attaatatct tcggctgatt
     1741 ctttaacttt ttccgcaggt atcttcatct ttttaccggt aattgcagcg acttcgcggg
     1801 caataccgat catactcaaa caatcggcac gattaggagt aatgccgata tctaaaacag
     1861 tatcgcc
//
LOCUS       DASVIM010000070         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1392078, whole genome shotgun sequence.
ACCESSION   DASVIM010000070 DASVIM010000000
VERSION     DASVIM010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392078"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..828
                     /locus_tag="VF305_01415"
     CDS             286..828
                     /locus_tag="VF305_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7415823.1"
                     /translation="MKPIGPLMWEHRLIEKMLASMMRHIDQVEKSKKVNPLIIDMAVD
                     FVRTYADRTHHGKEEEILFRDLAKKNLTPELKKIMQELLDEHVWARKTTGLLVAAKEK
                     YLQGDAEKLTGIIDLARQLGDFYPKHIEKEDKHFFYPCQEYFVKDEQEKMLAEFWEFD
                     RKMIHEKYNKVVEEYSGLSL"
     gene            complement(825..1478)
                     /locus_tag="VF305_01420"
     CDS             complement(825..1478)
                     /locus_tag="VF305_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415824.1"
                     /translation="MTPATQQALAGLRDLSMIKWYAISLLAFVFYVYVREIKEARQTK
                     NWDAVLAGLTIFGVDFFNETWNGWIMYLTQRSAFWTTPGDTALRTLVGWNIEIMFMFL
                     LLGIIYYHTLSKSKKEKILGMPEKWFWAIGYSAFCVFIECLLNIGGLLVWEYPFWNLS
                     LKGIWLIFFLGYFTFFCFAILVISLKKLKTKIMTVGIIYAVPILMNILAIGFLRWKY"
     gene            2096..>2315
                     /locus_tag="VF305_01425"
     CDS             2096..>2315
                     /locus_tag="VF305_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX7415825.1"
                     /translation="MEKIIEKMNNHYIICGGGRIGFLICRELIAGKMPCVVIDNNPEV
                     IQKAQDEGFICFKGDATQDKILIEAGIKR"
BASE COUNT          753 a          412 c          491 g          659 t
ORIGIN      
        1 ggcaagacgt tgcgatgttt tagaaaggcc ggtcttttgc aaagtaaaaa gggcatcttt
       61 atattttaca ttctttgaca tatgagttta atatcgcaaa tgcaacaaag ttgcaataat
      121 tatcgaggat aagttatggc gccaataaaa atatccacga cgaatacaat atttttggct
      181 attgagttga ttcatgctat acataattat caaactggaa aatttaacat actcacatgc
      241 aacaattgcc tcaacaatat aatcgtcagt aaaggaggat agtttatgaa gccaatcgga
      301 ccgttaatgt gggaacaccg gttaattgaa aaaatgctag cttcaatgat gcgccatatt
      361 gatcaggttg aaaagagcaa gaaggttaac cccctgatta ttgatatggc cgttgatttt
      421 gtgcgcacat atgccgaccg gactcaccat ggtaaggaag aggaaattct ctttagagat
      481 ttggccaaga aaaatctcac accggaatta aagaaaatca tgcaggagtt actcgatgag
      541 catgtatggg ctcgcaagac gacaggcttg ctggttgcgg ctaaagaaaa atatttgcag
      601 ggcgatgcag aaaaacttac cgggataata gacctcgcgc ggcaattagg cgatttttac
      661 ccgaagcata tcgaaaaaga agacaagcat ttcttttatc cctgccagga atacttcgtg
      721 aaagacgagc aggaaaaaat gctggccgaa ttttgggaat tcgaccgcaa aatgattcac
      781 gaaaaatata ataaagttgt cgaagaatac tccgggctca gcctttaata tttccagcgc
      841 aaaaatccga tagcgagtat attcatcaaa atcggcacgg cgtaaataat gcccacggtc
      901 atgattttag tttttagctt ttttaagctg atcactaaaa tagcaaagca gaagaaggtg
      961 aagtagccca agaaaaagat cagccagata ccctttaacg ataaattcca gaagggatat
     1021 tcccagacga ggagtccgcc gatattcaac agacattcaa tgaatacgca aaaagcgctg
     1081 tagcctatag cccaaaacca tttttccggc attccgagaa ttttctcttt tttgcttttc
     1141 gaaagagtgt ggtagtagat aatgcccaga agcagaaaca tgaacattat ttcgatattc
     1201 catcccacca gcgtccgcag cgccgtatcg cccggagttg tccaaaaagc ggaacgctgg
     1261 gttaaataca ttatccagcc gttccatgtt tcattaaaga aatcgacgcc aaaaatagtc
     1321 agaccggcca ggacggcatc ccagttttta gtttggcgcg cctcctttat ttctcgaacg
     1381 taaacataaa aaacgaaagc taaaagagag attgcatacc atttaatcat ggaaagatcc
     1441 cttagaccgg ctaaagcttg ttgggttgct ggtgtcatat gacctcctta gtaaatgtta
     1501 ataatgaaaa ttgtatttac tagtgaatca ttctattgtg ctatcactat catgctatcg
     1561 gatatattta attatactat aacattagat aaacaaaaag aaataatttg ccttaatttc
     1621 agtgctggat ttttatataa aatgtgttta aataagcttt tatagggtat aagaaattca
     1681 ttcaccccgt tagagatgta gtaacttgtg gcttattttt taatgaaagt tgcggatgac
     1741 aaatagtaga aggtatataa taatttcaac atctctaacg gggtaaagga gaaaagagct
     1801 tttgatactt accagcaaac tgaaaatcgc ttttttaatt ttgttcaccg tctttatagt
     1861 tggcactacc ggttttcatt ttatcgaagg atggagtttt attgactcct tttatactac
     1921 gattactact ttaacgacgg tggggtatgg tgatttcacg ccgagaacag cggaaggaaa
     1981 aatatttacg gtctttataa ttatttttgg cgtaggcacg atgctgtatt cgttcggcct
     2041 cgtttcggaa accttcattg aaggccgctt acgcagatta ttagggaggg gtaaattgga
     2101 aaaaataatc gaaaaaatga ataatcatta tattatttgt ggcggtggcc gcatcggttt
     2161 tttaatttgc cgcgaactaa ttgcggggaa aatgccgtgt gtggtcatcg ataacaatcc
     2221 ggaggtgatt cagaaggctc aggacgaggg ttttatttgt ttcaagggag atgcaactca
     2281 ggataaaatt ttaattgaag cggggatcaa acggg
//
LOCUS       DASVIM010000071         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_623628, whole genome shotgun sequence.
ACCESSION   DASVIM010000071 DASVIM010000000
VERSION     DASVIM010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623628"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1194)
                     /locus_tag="VF305_01430"
     CDS             complement(199..1194)
                     /locus_tag="VF305_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX7415826.1"
                     /translation="MIKKQKAIKRAKDVLKIEAQSILQLIDKIDSNFSCAVDLIYKSK
                     GRVIITGIGKSGLIGRKIVATLTSTGTQALFLHPVEGIHGDLGIVTKKDILLAISNSG
                     ETHELMPIINSVRYIGAPVIAFTGDMSSTLARTSDIVINVSVKKEACPFGLTPTSSST
                     ATLAMGDALAIALIDKRHFREKDFYKFHPGGSLGTRLKETVGEVMIIGKQIPKVLTGT
                     TAQKAIQEIDRKNVGFVLITDKKNKLLGILTDGDVRRMISKGISFEGKIIDNLMTRKP
                     KTIDENVSLAQTMELMQKEEITSFAVVNEKKELQGYIHLHDILGRGGSVVISLTQ"
     gene            1602..2375
                     /locus_tag="VF305_01435"
     CDS             1602..2375
                     /locus_tag="VF305_01435"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX7415827.1"
                     /translation="MKAVFISDAHLRRANDERYGKLINFFDDIKEGKVSLPADSDKTG
                     RGPVYIDDLYIVGDLFDFWFCRKDKIHPEFMLIINKLIELQKAGIRIHLNEGNHDFFM
                     GEYFRDVLGMEVFEEGANVKLDGLRALIAHGDTTDKTNIKYILFRKFLHGSLFYHLQR
                     FIPASFLWALAGFSSTASKEMTRKDGDTLVEKMLSFALPKFQIEYDAVILGHCHKPVL
                     RHYVVEGKKKTFVTLGDWIRHYSFLYYEDNNFFLGYYRP"
     gene            2431..3276
                     /gene="speB"
                     /locus_tag="VF305_01440"
     CDS             2431..3276
                     /gene="speB"
                     /locus_tag="VF305_01440"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX7415828.1"
                     /translation="MNFGGIYPAHSLRDALFVVVPVPYDLTSTYQPGSRRGPAAIIEA
                     STNMELYDEELKKETYLAGIHTTLPVTIDARGPKNMINVVRKKIAKIATLNKISVMLG
                     GEHSISLGAVQALKEKYPKLKVLQLDAHADLRESYQESPYSHASAARRIAEICPLIQV
                     GIRSMSKEEAEFLPQSKVKSYSADFALEKKDWAELICKDLSGDVFVTIDLDVFDPSIM
                     PSTGTPEPDGLYWRDVLRLLKLVSRSCKIRGFDVVELAPIPGIVAPDFMAAKLIYRIM
                     GYINK"
BASE COUNT          921 a          627 c          726 g         1027 t
ORIGIN      
        1 ttggatgctg gaacgatcac cgatcacaac cggcccaaaa tctgcccgga tggaaacacc
       61 gggtgcaatt aaacattcat gaccgatttt tacgtcacct attaaaacgg cattaggatg
      121 aacaaaagaa tcgactggta cttgcggact tttaccgtca aattcataga gagccataat
      181 tattgtcttt cagcttggtt attgagttag agaaataaca accgacccgc ctctgcctaa
      241 aatgtcatgc agatggatat atccttggag ctcctttttt tcgttgacca cagcaaaaga
      301 tgtaatttct tctttttgca tgagttccat ggtttgcgcc aatgaaacat tttcgtcaat
      361 agttttgggt ttgcgcgtca tcaaattgtc gataattttt ccctcgaaag aaattccttt
      421 actgatcata cggcgcacgt ctccatcagt caaaattccc agaagcttgt tttttttgtc
      481 ggtgattagg acaaatccca catttttacg gtcaatttct tgtatcgcct tttgggcggt
      541 agttcccgta agtactttgg gaatttgttt cccgataatc ataacttctc cgactgtttc
      601 ttttaatctt gtgccgaggc ttccgcccgg atgaaattta tagaaatctt tttctctaaa
      661 atgcctttta tcaattagag ctatagctaa agcatcaccc atcgctaaag tggcagtaga
      721 gctggaagta ggagtaaggc cgaaagggca cgcttctttt tttacggaaa cgttaatgac
      781 gatgtcgctt gttcgagcta gagttgaaga catgtcaccg gtaaaggcga tgacaggagc
      841 tccgatatag cggacggaat taatgatggg cataagttcg tgggtttcac cactgttgga
      901 aatggccagc aaaatgtctt ttttagtgac aatgcctaaa tcgccgtgga tgccttccac
      961 aggatggaga aaaagagctt gcgttcccgt actcgtcagg gtggcgacga tttttcgccc
     1021 gatcaacccg gatttgccga ttcctgtaat gattactctg cccttagatt tgtaaattaa
     1081 atccacagca caagaaaagt tgctgtcaat tttatcgatc aactgcaaga tactttgtgc
     1141 ttctattttt aaaacatctt tagcgcgttt gattgctttt tgttttttta tcatttttcc
     1201 taccttttac tgaagccgta aattttatat attatttatt ggaggctttg atgaaaacta
     1261 gcaaaaaaag ccagacaaaa caagaatcaa ttatgtctta ttcagttagg caattaactt
     1321 tttcagtgaa aagtatttac gggtattaag tctacgcgaa tcgtatcagg ggctttttaa
     1381 cggatgaatt ttaatttggt agcatgtaaa acattattat taataataag ttacaagaaa
     1441 ataacttcat cttccgaaaa ttaatgattc cttgtaagca ggataagaca gctaccaagt
     1501 aacctggttt tcaattattt ttaggggcaa tgcaggtgtt tattgacctt gcaggggtta
     1561 atctgctaaa gcatattgca ttaaaccgat gggattaaag tatgaaggct gtttttattt
     1621 ccgatgctca tttaaggcgc gctaatgacg aaagatacgg aaaactaata aacttcttcg
     1681 atgatattaa agaaggaaag gtcagtctcc cggccgattc tgataaaacc ggtagaggac
     1741 cagtctatat tgatgatctc tatatcgtcg gtgatttgtt tgacttttgg ttttgccgta
     1801 aggataaaat tcatccggaa tttatgctga tcattaataa actgatagaa ttacaaaaag
     1861 ccggtatccg catccatctg aatgaaggaa accatgattt ctttatgggc gaatattttc
     1921 gcgatgtttt ggggatggaa gtatttgaag aaggggcaaa tgtaaaacta gacggcttgc
     1981 gggcattaat tgcacacggc gacacaacgg ataaaaccaa cattaaatat atcttgtttc
     2041 ggaaattttt gcacggctct ctattttatc atcttcagcg ctttattccg gcgtcgtttc
     2101 tctgggcctt ggctggtttt agttctaccg ccagtaaaga aatgactaga aaagatggcg
     2161 atacgctggt tgaaaaaatg ctttcctttg ccttgcctaa atttcagata gagtatgatg
     2221 ctgtaattct cggccattgc cacaagccgg ttttgcgtca ttatgttgtg gagggaaaga
     2281 aaaagacttt tgttacgttg ggcgactgga tcagacacta ctcatttctc tactatgaag
     2341 ataataattt ttttctgggg tattaccggc cgtgattttt ggaaccttgt atagatgttc
     2401 caccattcag tattagtgag gattgaactt atgaattttg gaggaattta tccagcgcat
     2461 tcactgcgcg atgctttgtt tgtcgttgtt cccgttccgt atgatctgac ttccacttat
     2521 cagccgggga gccgccgggg accggcagcc atcatcgaag cttcgaccaa tatggagctc
     2581 tacgatgaag agttaaaaaa agaaacatat ttggccggaa ttcatactac tttaccggtt
     2641 accattgatg cccgcgggcc aaagaatatg attaatgttg tgcgcaaaaa aattgccaag
     2701 attgccactc taaataaaat atcggtaatg ctgggtggag agcacagcat cagtttgggg
     2761 gctgtgcagg cccttaaaga aaaatatcct aaactaaaag tgctgcaact ggatgcccat
     2821 gccgacttgc gggaaagtta tcaggaaagc ccttacagtc acgcctccgc agccagaaga
     2881 attgcggaaa tatgtccgct gattcaggtg ggcattagga gcatgagtaa agaagaagcg
     2941 gagtttttac cgcagagcaa agttaaaagc tatagtgcag attttgcttt agagaagaaa
     3001 gactgggctg aattaatttg taaagacttg agcggtgatg tttttgttac tattgattta
     3061 gatgtttttg atccttccat aatgccttcc acgggtacgc cggagccgga cggtctttac
     3121 tggagggacg ttttgcgttt gttgaagttg gtttcgcgtt cctgtaaaat ccgtggtttt
     3181 gatgtggtag aactagcacc cattcccggt atagttgcgc cggatttcat ggcggcaaag
     3241 cttatttacc gtattatggg ctacattaat aaatagctat ttcctgtcag atcgcctttg
     3301 a
//
LOCUS       DASVIM010000072         2820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_198092, whole genome shotgun sequence.
ACCESSION   DASVIM010000072 DASVIM010000000
VERSION     DASVIM010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198092"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..373)
                     /locus_tag="VF305_01445"
     CDS             complement(92..373)
                     /locus_tag="VF305_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7415829.1"
                     /translation="MNKSGLIETLATKLNLTEKKAIDVVNLVFKGFTDELKKSGRIEI
                     RGFGSFIVKKYGAYTGRNPKTGTKIQVEPKKLPFFKVGKELKDRVDKKK"
     gene            complement(485..1216)
                     /locus_tag="VF305_01450"
     CDS             complement(485..1216)
                     /locus_tag="VF305_01450"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX7415830.1"
                     /translation="MKERLQKIIAAAGVASRRHAEKLITGGRVSVNNIVVTELGVKAD
                     AEKDIIRIDGKTISVEKTKLYIALNKPAGFVTTLHDPQNRPTVVDLLSAVPERVYPVG
                     RLDYDSAGLLLLTNDGDFAQKIQHPRFQMSKVYRVKIQGHLSREEVKQLSKGIKLSDG
                     VFKPENVRIEKINDRSCWFRLTLKGGKNRIIRRGFEAIGHRVALLVREAIGDLKLGNL
                     KEGFWRYLTKKEINQLLSNGANEDR"
     gene            complement(1213..1767)
                     /gene="scpB"
                     /locus_tag="VF305_01455"
     CDS             complement(1213..1767)
                     /gene="scpB"
                     /locus_tag="VF305_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX7415831.1"
                     /translation="MENIKAVIEALVLASEAPLVLEKICLILPEVEKTEIKEALEKLV
                     TEYDERQAGICLQEVAGGFQFRTRPELSNWVKKLKSTKPASLSSAAMETLAIIAYRQP
                     IVKAEIESIRGVDVGAPLKGLLEKKLVRIVGRKDVPGKPIIYGTTKKFLEVFNLKGLS
                     ELPTLRELKELHEQQETSEDFSNS"
     gene            complement(1768..2511)
                     /locus_tag="VF305_01460"
     CDS             complement(1768..2511)
                     /locus_tag="VF305_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX7415832.1"
                     /translation="MNNEQTINYAIKLDIFEGPLDLLLYLIKKNEIDIYNIPVALVTE
                     QYLQYLKMIKSLNLDLAGEYLVMASTLIHIKSKMLLPVPEEPEEEDEKDPRAELVRQL
                     VEYQAFKEAAANLGKRPLLERDVFTRAALMPEEIEKLNADEEELMEVSIFELMEAFHR
                     LVSRIDKQELLEIDLEKISLTDIINDVMERLSYAKNLTFDELLGERKDRRRIVYTFLA
                     LLELIKLRMIKAYQTSVFGVIRIFPAVES"
BASE COUNT          744 a          669 c          519 g          888 t
ORIGIN      
        1 taatttgatt gaactatagc aataaacgag gaaaaggggg cttgaatcaa gtcccctttt
       61 ttattctata tagacaaagg caagggtttt gctatttctt cttatcaact ctatccttca
      121 gttcttttcc tactttaaag aagggtaatt tcttcggctc aacttgaatt ttagtgccgg
      181 ttttaggatt tcttcccgtg taggcgccgt atttctttac tataaaactg ccaaagcccc
      241 taatttcaat acgcccgctt ttttttaact catcggtaaa acccttaaaa acaagattaa
      301 caacgtcaat tgcctttttt tccgtcaagt ttaacttagt agccaaagtt tcaataagac
      361 ctgatttgtt catataccct ccttaagact agttacaacc ctgaatcgcc agatgataat
      421 aatatatgtt tatttccatg tgttagagtt tattatttat tttccggcga atgtcaagaa
      481 aattttacct gtcctcgttt gccccattac tgagtaactg gtttatttct tttttggtca
      541 aataccgcca gaagccttcc ttcagattgc ctagcttcag atcgccgatt gcttcccgca
      601 ccagaagggc tacgcgatga ccgatagctt caaaacctct cctgatgatt ctatttttcc
      661 ccccttttag agtaagccga aaccagcagc tccgatcatt gatcttttct attcggacat
      721 tttccggttt aaaaacacca tcggacaact taattccctt gctcaactgt tttacttctt
      781 ctctggacaa atgtccctga attttgactc tgtatacttt ggacatctgg aaacggggat
      841 gctggatttt ttgtgcgaaa tcgccatcat tagtcaaaag caaaagccct gcggaatcgt
      901 aatcaagcct tcctaccgga taaacccttt ccggcacagc gcttagcaaa tccacaactg
      961 tcggcctatt ttggggatca tggagggtgg taacaaaccc tgccggttta tttaaagcaa
     1021 tataaagttt cgttttttct acagaaatag tttttccgtc gattctaata atatcttttt
     1081 cggcatcagc ctttactccc aactcggtta ccacaatatt attaacgctt acgcgcccac
     1141 cggttattag tttttccgcg tggcgccgtg aggcaacacc ggcagcagcg ataatttttt
     1201 gcaaacgttc tttcatgagt tggaaaaatc ttcgctggtt tcctgctgct cgtgaagttc
     1261 cttcaattcc cgtaaagtcg gcaactccga gagacctttg agattaaaaa cttccaggaa
     1321 tttcttagtg gtcccataaa ttataggttt gccgggcaca tccttacgcc ccacaatgcg
     1381 cacgagcttc ttttccagaa gccccttgag cggagcgccc acatccactc cacgaatact
     1441 ttcaatttca gctttaacaa tgggctgcct ataagcaata atcgccagag tttccattgc
     1501 tgccgaagat aaagaagcgg gctttgtact ctttaacttt tttacccaat tgctaagttc
     1561 gggccgggtc cggaattgaa agcctccggc aacttcctgc aaacaaatac ccgcctgccg
     1621 ttcatcatat tccgtcacaa gtttttcgag ggcttcttta atttccgttt tttcaacttc
     1681 cggaaggatc aggcaaattt tttcgagcac caacggcgcc tcggaagcta aaaccagagc
     1741 ttcaattact gctttaatgt tttccattta tgactccaca gccggaaata tccggatcac
     1801 accaaaaact gaagtttgat aagctttaat catcctgagt ttaattaatt ccagcaaagc
     1861 taaaaaagtg tagactatgc ggcggcgatc ttttctttcc ccaagcaact cgtcaaaagt
     1921 cagatttttt gcgtaagaca accgttccat tacatcgtta attatatcag taagcgatat
     1981 cttttccaga tcaatttcca aaagttcttg tttatcgatt cgggaaacaa gacgatgaaa
     2041 agcctccata agttcaaaaa tacttacttc catgagttct tcctcgtccg catttaattt
     2101 ttcgatctct tccggcatga gcgctgcccg ggtaaacaca tcccgctcca aaagcggtct
     2161 ttttcccagg ttggcggcag cttctttaaa agcctgatac tctacaagct gtctgaccag
     2221 ttcggcgcgg ggatctttct catcttcctc ttccggctct tcgggcacag gcaaaagcat
     2281 cttggatttg atgtgaataa gggttgaagc catgaccaaa tactcaccgg ccaaatcgag
     2341 attgagtgat ttgatcattt tgagatactg aagatactgt tcggtaacta gagctacggg
     2401 aatattatag atgtcaattt cgtttttttt gatcagatac agaagcaagt cgagcggacc
     2461 ttcaaaaata tccagcttaa ttgcatagtt tatcgtttgt tcgttattca taattaatcg
     2521 ggagtgcagc ctgcaaatac cggcttatag tcatagtagc gcgctctaat cattataagg
     2581 tttctcgctt aatccaaaat ggcaactctt ggcatccaga agctcagccc aatgggcaga
     2641 ggacttcact attaaccaat tagatattaa tcgcagcgcg gacttcatcc attgtttgac
     2701 gcgctatctt tgttgctttc ctattgcctt cttctataac cgcgttaatt tcattcaaat
     2761 gatttatgta ataatccatc cgcgcgtgaa tgggctgcat agcttcggca atacgccggg
//
LOCUS       DASVIM010000073         3442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1136498, whole genome shotgun sequence.
ACCESSION   DASVIM010000073 DASVIM010000000
VERSION     DASVIM010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136498"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VF305_01465"
     CDS             <1..630
                     /locus_tag="VF305_01465"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7415833.1"
                     /translation="TCAIDLGCGSGIIPLILAKNFLGVNWVGLEIQEELAALAQKNIQ
                     LNGFNSRIKILTGDARKIKNIFPAQSFDIVTFNAPYRKLSSGRINPQLEKAIARHEIK
                     GSLKDFLKAAKYLLKPAGKVFTIYPAKRLVELISLFRDSAIEPKRMKLVFSDHFSEAE
                     FVLVEGRNGGREELKIEQPLFIYDQDKNCTKDMMSIFSELTRFPAGDDG"
     gene            complement(599..1090)
                     /locus_tag="VF305_01470"
     CDS             complement(599..1090)
                     /locus_tag="VF305_01470"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX7415834.1"
                     /translation="MRKKNYRARLYSIGEILFPILKKRGLAVKLEENALLKLWPKAVG
                     HQIALQTQPDCLKNRTLFVKTTSSVWVQQLHFMKEEILQKLNELAGKLAVKDIRFSIG
                     HKLAQAKTRANISATKKSLLKERDKKMIADCTATLADRELAAIFKRVMKIEISRRRQR
                     ETG"
     gene            complement(1216..2346)
                     /locus_tag="VF305_01475"
     CDS             complement(1216..2346)
                     /locus_tag="VF305_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7415835.1"
                     /translation="MESIGTLAGGIAHDFNNLLMGIQGHASLALLHLDPQNANYEHLK
                     TIETLVMSGADLTKQLLGFARGGKYEVKTVDLNLLIQKTSETIGRTKKEIKIHLNFQD
                     DLWPVDVDHGQIEQVLLNLYVNAWQAMPGGGDLYLETRNVFVEENRFRPFVPKNGHFV
                     KISITDTGVGMDEKTKERIFEPFFSTKEMGRGTGLGLASVYGIIKNHNGFINVYSEKG
                     QGTTFNIYLPESIKRDGKDKENKTDETNEIIKGAGIILLVDDEPMILNVGRKILETLG
                     YTIITAQDGTSAIELYKQSKKSIDLVILDMVMPEMGGGEVFDEIKKINPEVKVLLSSG
                     YSLNGQASRIINRGCVGFIQKPFTIRDISSQLHKIIDKQKPQ"
     gene            complement(2359..3180)
                     /locus_tag="VF305_01480"
     CDS             complement(2359..3180)
                     /locus_tag="VF305_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7415836.1"
                     /translation="METKPNYEDLEQKIKELEQIILRYENTENQYRYLVDSTTDSLYL
                     VDEKCRYIFMNNNHIQRLKLPIEKIIGHSYDEFHSAEQSKEFAEKVKEAFATGNSIKD
                     EHQSIRDGGYFLRTFSPVRESTGTNKIIAVAVVSKNITERRHTEEALYKSEEKYRQLI
                     ENSSEAIFIIANGQIKYANSRTQQMMGYETEELSLILFIDLVISEDRDMVLERQKNSL
                     HGDDSNINYTFRIMNKKGVTHWVETNSIGINWDNHPATLNFMRDMTRQKKTETRL"
     gene            complement(3200..>3442)
                     /locus_tag="VF305_01485"
     CDS             complement(3200..>3442)
                     /locus_tag="VF305_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX7415837.1"
                     /translation="LLQSAYKKSLELAREKGLQSVSFPAISCGIYGYPLEEAARLALK
                     TCIEFARSNTDIKIIRHILFSRPIYEVYSAELKKLV"
BASE COUNT          940 a          678 c          668 g         1156 t
ORIGIN      
        1 acctgcgcca ttgatttagg atgcggcagc ggtattattc ctcttatttt ggctaagaat
       61 ttcctcgggg ttaattgggt tggtttggaa attcaggaag aacttgcagc actggcgcaa
      121 aaaaatattc aactcaatgg cttcaatagt cgcatcaaga tattaaccgg cgatgcgcgc
      181 aaaataaaaa atatttttcc tgcacagtca ttcgacattg taacatttaa tgcgccttat
      241 cgaaaattaa gctccggcag aattaatcct cagctcgaaa aagccattgc ccgtcatgaa
      301 atcaaaggtt ctttaaaaga ttttctaaag gcggcaaaat atttgctcaa accagcggga
      361 aaagtattta ctatttatcc ggcaaaaaga ttagtggaac taatttccct tttccgcgat
      421 agcgccattg aacccaaaag aatgaagctg gtattttccg atcatttttc tgaggcggaa
      481 tttgttttgg tcgaaggcag aaacggcggc cgtgaagagc tgaaaattga acagcctctt
      541 tttatttatg atcaagacaa aaattgcact aaagatatga tgagtatttt tagcgaactt
      601 acccggtttc ccgctggcga cgacggctga tttctatttt cataacccgt ttgaagatag
      661 cggccagttc tcggtcggcc agtgtggccg tgcaatccgc aatcattttt ttgtctcttt
      721 ctttcaataa agattttttt gttgcagata tattcgctct cgtttttgct tgggcaagtt
      781 tgtggccgat tgaaaaacgt atatctttta cagcaagttt tccggcaagt tcattaagtt
      841 tttggagaat ttcttctttc atgaaatgaa gttgttgcac ccataccgag gaagttgttt
      901 tgacaaataa agttctgttt tttaagcaat caggttgcgt ttgcaaggcg atctggtggc
      961 cgacagcttt aggccaaagt ttcagcaggg cattttcttc gagttttaca gctagccccc
     1021 tttttttcag gatcggaaaa agaatttcgc ctattgaata caatctggcc cggtaatttt
     1081 tcttgcgcat gtttttttgt ggcacaattt gaaaaatagg tcaaggaaac ggaaagaaga
     1141 aaacaagatt atgtaataat aacataggcg aaaaattatc accaaaatat attaatcaca
     1201 aattgttatt taattttact ggggtttttg tttatcgatg attttgtgca actgactgga
     1261 gatatcacgg atagtaaatg gtttttgaat aaaaccaacg caaccgcgat taattattcg
     1321 gctggcctga ccgtttaaac tgtaaccgct ggagagtaag actttcactt ccggattgat
     1381 ttttttgatt tcgtcaaaaa cctctcctcc gcccatttcc ggcattacca tatctaagat
     1441 tactaaatct atgctctttt tagactgttt gtataattct attgcggaag taccatcttg
     1501 agccgtgatt attgtatagc ctaaggtttc taatattttc ctgcctacat tcaaaatcat
     1561 gggttcatca tcaacgagaa gaattattcc cgcgcctttg ataatttcat tagtctcatc
     1621 cgttttattt tctttatcct ttccatccct tttaattgat tccggaagat aaatgttgaa
     1681 agttgtgccc tgtccttttt cggagtagac attgataaag ccattgtgat tcttaataat
     1741 tccgtaaaca gaggcgagtc ccaaaccggt tccacgaccc atttctttag tcgaaaaaaa
     1801 tggttcaaaa attctttctt ttgttttttc atccatgcca acgcccgtat cggttattga
     1861 aatttttaca aaatgaccat tcttagggac aaatggtcta aaccgatttt cttcgacaaa
     1921 aacatttctg gtttctaaat aaaggtcacc gccgccgggc atagcttgcc aggcattgac
     1981 ataaagatta agcaacactt gttcaatttg tccatgatca acatcaaccg gccaaaggtc
     2041 gtcttgaaag ttgaggtgga ttttaatttc ttttttcgtg cggccaattg tttctgaagt
     2101 tttttgaatt aacaaattta aatctacagt ctttacctca tatttaccac cgcgggcgaa
     2161 acccaataat tgtttggtta aatctgcgcc gctcatgaca agagtttcta ttgttttcag
     2221 gtgttcataa ttagcatttt ggggatccag gtggagaaga gccaaagaag catgtccctg
     2281 aatgcccatt agcagattgt taaaatcatg ggcgatacct ccggcaagag tgccgatgga
     2341 ttccattttt tgggcttgtt aaagtcttgt ttccgttttt ttttgtctcg tcatatcgcg
     2401 cataaaattt aaagttgccg gatgattatc ccaatttata ccgatactat tagtttccac
     2461 ccaatgtgtt actccttttt tattcattat tctaaaggtg tagttaatgt tggaatcatc
     2521 tccgtgcagg ctattctttt gacgttcgag aaccatgtcg cgatcctcgg aaataactag
     2581 atcaatgaat aagattaatg acagctcctc cgtttcgtag cccatcattt gttgagtgcg
     2641 cgagttggca tattttattt gtccattggc aataataaaa atggcttcgc tggaattttc
     2701 aatgagctga cgatactttt cttccgattt ataaagcgct tcttcggtat gccgcctttc
     2761 cgtaatattc ttggagacaa cggcgacagc aattatctta ttggtacctg tggattccct
     2821 taccgggctg aatgtgcgta agaaataacc gccgtcacga atactttgat gctcatcttt
     2881 aatagaattg cccgtcgcga atgcttcttt aactttttcg gcaaattctt tgctttgttc
     2941 ggcagaatga aactcatcgt atgaatgtcc gataattttt tctatgggca atttcaatct
     3001 ttgaatatgg ttattattca taaaaatata acggcatttc tcatcaacca gatacaaaga
     3061 gtcggtagtt gagtcaacga gatagcgata ttgattctcg gtgttttcat accggagaat
     3121 tatttgctct aattctttta ttttctgttc gagatcttcg taattcggtt tggtttccat
     3181 cttcgcacca tgtgtgtgac taaaccaatt ttttcaattc cgcggaataa acttcataaa
     3241 ttggccggct gaaaagaata tggcgaatga tctttatatc cgtattgctt cgggcgaatt
     3301 caatacaagt cttcagcgcc aaacgagctg cttcttccag aggataccca taaataccgc
     3361 agcttatagc cggaaatgag acgctttgca atcctttttc tcgggcaagt tccaggcttt
     3421 ttttgtaggc gctctgcaga ag
//
LOCUS       DASVIM010000074         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_538674, whole genome shotgun sequence.
ACCESSION   DASVIM010000074 DASVIM010000000
VERSION     DASVIM010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538674"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VF305_01490"
     CDS             <1..530
                     /locus_tag="VF305_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7415838.1"
                     /translation="EVAVISLAGIVYSKSVRVAGDKIDEAIVQYMKRKYSLLIGERTA
                     EITKIEIGCAYPLEESKTCEVKGRDLIAGVPKIIECTSEEIREAINEPLTLIIDAIKD
                     VFEHSPPELAGDIVDRGIVLTGGGALLQNIDTFVREETGLPITITDDPLSAVARGAGM
                     ALDQLDVLKEIAIQV"
     gene            754..1581
                     /gene="mreC"
                     /locus_tag="VF305_01495"
     CDS             754..1581
                     /gene="mreC"
                     /locus_tag="VF305_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX7415839.1"
                     /translation="MFFLRNYKTVIFIVVLLFISLIMLSYNLKHETDKGFFRKIVLEA
                     AAPVQKVLSVSVKSVSDAWMRYIFLVGLEEENKILKKEINELKAELILYQEGYLEAQR
                     LRKLLSLRDNYNYHFVSAGVIGREQAALSKTILINKGTVHGLKVGMPVIAPPGLIGRL
                     IDVSWHASKILLLIDESSNIDAIVQRTRTQGIVCGAGSRGCILKYISKNQDVKKGDVI
                     VSSGIGVVFPKGLLIGIVSHVDQQNSGLFWTINVAPFVDFSKLEEVLVLASEEAESE"
     gene            1584..2087
                     /gene="mreD"
                     /locus_tag="VF305_01500"
     CDS             1584..2087
                     /gene="mreD"
                     /locus_tag="VF305_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX7415840.1"
                     /translation="MLYYLSLPFLSILLVVLQTTIADIIFSGRLVVELTLIAVIYAGF
                     RLDLIRGSILAFILGFVFDCVTGSVLGLFTFVYVVIFLCSFFASFRLGTEKMYIISLF
                     SFICAFLEELIVSLFYYLAFKFNILTNIPFVFLPQALIIGLLAPVFFYLMRWVEVFFY
                     GKTVQPA"
     gene            2062..>2737
                     /locus_tag="VF305_01505"
     CDS             2062..>2737
                     /locus_tag="VF305_01505"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415841.1"
                     /translation="MEKRYNLLSGQEPAEFRQKLKIFIVLLLIALSILLVRLWYLQVI
                     KGEELKQRSESNSVRYRKIQPLRGLIMDNKGHVLVNNRPSFDILYMPGQGKDHEQLME
                     RLKDLYKSKSLELSYDQPFPKTWKPYLPVRLEKNVSMAKVALVETNALDLPGVYVDVS
                     PVRLYMDGEMMAPVVGYTGEISKEELEKGDDENAYGDVSGKHGVEKFFDSYIRGRNGA
                     EVVEVNV"
BASE COUNT          767 a          469 c          620 g          881 t
ORIGIN      
        1 cagaagtcgc cgttatttcc cttgccggca ttgtgtattc gaaatctgtg agggttgccg
       61 gtgataagat tgatgaagca atcgttcaat atatgaagcg gaaatatagt ttacttatcg
      121 gagaaagaac agcagaaatt accaaaattg agattggctg tgcctatccc ttggaagaaa
      181 gtaaaacttg tgaggtaaaa gggcgggatt tgattgcggg agttcctaaa atcatcgaat
      241 gtacttcgga agaaatcagg gaagccatca acgaaccgct tacccttatt atcgatgcaa
      301 ttaaagacgt ttttgaacat tcaccaccgg aacttgccgg ggatattgtc gatcgtggta
      361 ttgttcttac aggtggcgga gctctactgc aaaatattga caccttcgtc agagaagaga
      421 ctggtttgcc tataactatt actgatgatc ctttatctgc cgtagctcgt ggtgcaggca
      481 tggccttaga tcaactcgat gtgctcaaag agattgctat tcaagtataa aatcgtctaa
      541 gggcataata aaatggataa taatttattt tattcattgt tgtttttgta tatctacgaa
      601 attgctgctg tagctgtaag ataagtcagg ttaaaaaagc gctataggat tcataatgtc
      661 ccttgatgga ctggtaaata agttctgcaa accttgtatt ctggcagttg gcgtttgatc
      721 cggcgttatg attattttcc taataggtaa ataatgtttt ttttaaggaa ttacaagact
      781 gttatattca ttgttgttct tcttttcata tcactgatta tgttatccta taatttaaag
      841 catgaaacag ataaaggttt cttcagaaaa attgtattag aggcagctgc acctgtgcaa
      901 aaagttttga gcgtttctgt taaaagtgta agtgatgctt ggatgcgcta tatttttctt
      961 gttggtctcg aagaagaaaa taaaattcta aaaaaggaaa ttaacgagct caaagcggaa
     1021 ctgattttat atcaagaagg ttatctggaa gcgcaaaggc taagaaagct tctatcccta
     1081 agagataatt ataattacca ttttgtttcc gccggggtta tcggcagaga acaggccgcc
     1141 ctatctaaga cgatattgat caataaaggt accgttcatg gcctgaaggt cggcatgccg
     1201 gtcattgcac ccccgggatt aattggccgc ctgatagatg tttcctggca tgcttccaaa
     1261 atattgttac ttattgatga aagcagtaac attgacgcta ttgtgcagcg gacgcggacg
     1321 caaggaatcg tctgtggagc aggctcccgc ggatgcatat tgaaatatat ttctaaaaac
     1381 caggatgtta aaaaaggtga tgtcattgtt tcttccggca taggcgttgt atttcccaag
     1441 ggcttgctaa tcggcatcgt cagccatgta gaccagcaaa attctggatt attttggaca
     1501 attaatgttg ctccgtttgt tgatttttcc aaattggaag aagtattagt cttagcttcc
     1561 gaagaagcgg aaagcgaata ataatgcttt attatttatc gctgccattt ttatcaatat
     1621 tattggtggt gctgcaaaca actattgccg atataatctt ttccggccgt ttggtggtag
     1681 aacttacact aattgccgtc atttatgctg gatttcgtct tgatctaatt agaggatcaa
     1741 tcttagcatt tattctcgga tttgtctttg attgtgttac cggttcggta ctggggttat
     1801 tcacttttgt ttatgtcgtc atttttctat gttcattttt tgcttctttc cggctgggta
     1861 ccgaaaaaat gtatattatc tccttgttta gttttatttg tgcttttctg gaagaactta
     1921 tagtgagttt attttattat ttagctttta aatttaatat attaacaaac ataccttttg
     1981 tttttttacc gcaggcgttg attatcggcc tacttgctcc tgtatttttt tacctgatgc
     2041 gttgggtaga ggtgtttttt tatggaaaaa cggtacaacc tgcttagcgg acaggaacca
     2101 gccgaatttc ggcagaagct taaaatattc atcgttttat tgttgattgc cctttccatt
     2161 ttattggtaa ggctgtggta tttacaggtt atcaaagggg aagaattgaa acaaagatcg
     2221 gaaagcaact ctgtccgtta ccgtaaaatt cagcctttgc gcggtctgat tatggacaac
     2281 aaggggcacg tgctggtgaa taatcgtccc tcctttgata tactttacat gcccggccag
     2341 ggaaaggacc acgagcagtt aatggaaagg ctcaaagacc tctacaaaag taaatcgctg
     2401 gaattgtctt atgaccagcc ttttcctaaa acatggaaac catatttgcc ggtaagattg
     2461 gagaaaaatg tgagcatggc aaaagttgct ttagtggaaa ccaatgctct ggatttgccg
     2521 ggagtctatg tcgatgtttc acctgtacgc ttgtatatgg atggagaaat gatggcgccg
     2581 gttgttgggt atacaggaga aataagcaaa gaggaattgg agaaaggtga tgacgaaaac
     2641 gcctacggtg atgtttcagg aaagcatggc gtagaaaagt tcttcgattc ttacatccgc
     2701 ggccgtaacg gcgcggaagt ggtcgaggta aatgttt
//
LOCUS       DASVIM010000075         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_795942, whole genome shotgun sequence.
ACCESSION   DASVIM010000075 DASVIM010000000
VERSION     DASVIM010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795942"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..576
                     /locus_tag="VF305_01510"
     CDS             211..576
                     /locus_tag="VF305_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415842.1"
                     /translation="MSIVKKIALYIARKEFCRKAIAEHANLSVFKKKPSFRIFIGLVL
                     IAFSYLIGLPAAIVLSIIGASQGNAIIAAVAAPLLYGISWLIFLVAMYLAGPEYGRAL
                     SRWAARVILEKILGDEMKT"
     gene            666..938
                     /locus_tag="VF305_01515"
     CDS             666..938
                     /locus_tag="VF305_01515"
                     /inference="COORDINATES: protein motif:HMM:NF006617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX7415843.1"
                     /translation="MSQLNELIAELKVKIIQTLGLMDVKPENIKDDAPLVGGDTGIDS
                     IDVLELVMMIEKDYGVKIESKELGAKVFASVRALAAHILANQRQKA"
     gene            993..>1621
                     /locus_tag="VF305_01520"
     CDS             993..>1621
                     /locus_tag="VF305_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX7415844.1"
                     /translation="MMKVLLISANTLRVPYPVYPLGLDYVAGSLDSRYSTRIVDLNEF
                     NDLEELGVIIREFSPDFIGVSIRNIDNTDTINSRGFLAEYQNLVRQIRKNSEAKLILG
                     GSGFTVFPVELLHALDADYGITGEGERLPLLLEALQKNAEVKSIPGIVTKETKGLQYE
                     PWNGTIKRGFNPASSHTQFYLSYGGMLNLQTKRGCPFRCSYCTYPYIEG"
BASE COUNT          465 a          292 c          366 g          498 t
ORIGIN      
        1 aaattctatc ggcggaatga tggtaaggtg atatatattc tgatattgtc tttaacggcg
       61 gttgttgatt ttaatcttca accgtctttt tacatctgta agctataatg ttctttattt
      121 atacttgata tataaatttt ccttgaaatt attttccact taaagctatt atcacacact
      181 tttttaaatt cgattgtttg cggggatctt ttgagtatcg taaaaaaaat agcattgtat
      241 attgcccgga aagagttctg ccggaaagca attgctgagc atgcgaacct cagcgtcttt
      301 aaaaagaaac cttcttttcg aatatttatc ggcctggttt tgattgcctt cagttatctt
      361 atcggtctgc cggcagcaat tgttttgagt ataattggtg catcacaggg caatgccata
      421 atagccgctg ttgctgctcc gctgctttat ggtatttcct ggctgatttt tttggtggcc
      481 atgtatttag cgggacctga atatggaagg gctttaagcc gctgggctgc gcgggttatt
      541 ctggaaaaaa ttctcgggga cgagatgaaa acataagctt ctttaaatac gaataatttc
      601 ggaagcataa agataaaaga ctaaaatgcg ctggctagtg ccagattaaa attttgaggt
      661 gttcaatgag tcaacttaat gaattaatag ctgaattaaa ggtgaagatc attcaaactc
      721 tgggcttaat ggatgttaaa ccggaaaata tcaaagatga tgctccgctt gtgggcggcg
      781 atacgggaat tgattccatt gatgtgttgg agttagtgat gatgattgaa aaagactatg
      841 gcgtcaaaat cgagagcaaa gaactgggcg ccaaggtttt tgcatcggtg cgagcattgg
      901 ccgcgcacat tttagcgaat cagcgtcaaa aggcttaagc tttcgcttac gcaaagataa
      961 taaccttata gcgctttttg catttttcct ctatgatgaa agtattgcta atctcggcaa
     1021 acactctgag agttccatac cccgtttatc cgctggggct cgattacgtc gccggttcat
     1081 tggattcccg ttattcgacc agaattgttg atttaaatga atttaatgat ctggaagaat
     1141 tgggggtgat aattcgagag ttttcacccg attttatcgg agtatcaatc agaaatatag
     1201 ataacacgga tacaataaac agccgcggct ttcttgcaga atatcaaaat ttggtgcgtc
     1261 agatcagaaa aaattctgaa gctaagttga ttttaggagg cagtggtttc actgtctttc
     1321 cggttgaact tctacacgcg ctggatgccg attacggcat taccggcgag ggcgaaaggc
     1381 tgcccctgct cttggaagcg ttgcaaaaaa atgctgaagt gaaatcaatt cctggcattg
     1441 taactaaaga aacaaaaggg ttacaatacg aaccatggaa cggtacaata aaaagaggct
     1501 ttaatccggc aagttcacat acacaatttt atttgtccta cgggggaatg ctcaacttgc
     1561 aaaccaagag gggctgccct tttcgttgca gttattgcac atatccctat attgaaggca
     1621 g
//
LOCUS       DASVIM010000076         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1393368, whole genome shotgun sequence.
ACCESSION   DASVIM010000076 DASVIM010000000
VERSION     DASVIM010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393368"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /gene="lpxD"
                     /locus_tag="VF305_01525"
     CDS             complement(<1..844)
                     /gene="lpxD"
                     /locus_tag="VF305_01525"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX7415845.1"
                     /translation="MKITLKEIAKILDGTIIGAEEVIINDIRSIEEANEGDLTFIANK
                     KYFKKLKSTKASAILAPPQTAAEGKNLIIVADPYAVLGKLLALFYPLEHGHSGISPDA
                     YIEDGATVSPEATVFPRAFISKGVKIGKGAVIYPGVYLGRNAAVGENSLIYANVTVYH
                     DCIIGKRVILHSGVVIGSDGFGFASPGVDNAKIPQVGFVQIDDDVEIGANSTVDRATM
                     GKTWIKRNVKIDNLVQIAHNVVIGENSVIAAQVGISGSTKLGKSVIVGGQAGMVGHLN
                     IGDNV"
     gene            complement(860..1381)
                     /locus_tag="VF305_01530"
     CDS             complement(860..1381)
                     /locus_tag="VF305_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX7415846.1"
                     /translation="MKRNIYLIAGLVLLLFVWSSNAFAAEKIGVINIREIMQNSIAGK
                     KAAEDLKSLADKRSAPIKSLENELKKMKDELDKQGSIMTQNARRDKEEAYQKKLRDYQ
                     LLVNDTNTELQKRDQEVFQKLMPEILKAVRVIAEKENYILVIDIATTPVPFHAKEIDF
                     SKKVIEEYNKTKK"
     gene            complement(1449..>2476)
                     /locus_tag="VF305_01535"
     CDS             complement(1449..>2476)
                     /locus_tag="VF305_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX7415847.1"
                     /translation="KMDINIRVKEKNTGMFMVGAGYSATDQALIMAQIVQQNFLGYGQ
                     TLSLKGGISATSSSIDLSFTEPWLFDLPLWCKVDIWKYTQLYDSYTLDTRGAGFTLGY
                     PLWEKITGYIGYKLSFDDIKDINYATASSYIIAQAGERITSAITLTLTRDTTDDYIFP
                     SKGTKSSISVEHAGDPLGGNVNLTKYGASLAAYYPLIWDTVFGARGRIGFLQNNDVDD
                     SKIPIYERYVLGGINSLRGLRYVGPTNPGTSDVIGGTTMLNFNFELVFPLIKDAGMKG
                     VVFYDAGNTWNGGYYIDDLRQTVGLGIRWYSPIGPLRLEYGYVLDRKGLNDDSIGRWE
                     FTIGMFM"
BASE COUNT          688 a          570 c          473 g          745 t
ORIGIN      
        1 ttacattgtc gccaatatta agatgtccga ccatacccgc ctgtccgccg actattacac
       61 ttttgcctag cttagtactt ccggaaattc caacttgggc ggcaatgact gagttttcac
      121 caatcaccac attgtgagca atttgaacaa gattatcaat tttaacattg cgcttaatcc
      181 acgtcttacc cattgtagcg cggtcaacgg tgctattggc gccgatttca acatcgtcat
      241 ctatttgcac aaagccaact tgtggaattt ttgcattatc gacaccaggc gaggcaaaac
      301 caaaaccatc agagccgatg acaacgcccg agtgtaaaat aacccgtttg ccgatgatgc
      361 agtcgtgata aacagtaacg ttggcataaa ttaacgagtt ttcgcctacc gcagcgttac
      421 ggccgaggta aacgccaggg taaataacag ctcccttacc gatctttact cccttactga
      481 taaaggctcg gggaaacact gttgcttcag gagatacggt agcaccatct tcaatataag
      541 cgtcaggact tatgccacta tgaccatgtt ccaacggata aaaaagcgcc aataattttc
      601 ctaaaacggc gtaggggtcg gccactataa tcaggttttt gccctccgcc gctgtctgcg
      661 gcggagcaag aatagccgat gctttggtcg acttcaattt cttaaagtac ttcttattgg
      721 cgataaaagt aagatcacct tcatttgctt cctcaattga ccggatatcg ttgatgatga
      781 cttcctctgc accaataatt gtgccgtcaa gaatcttagc tatttcctta agagttattt
      841 tcatcttaat aaaagttttc tatttttttg tcttattata ttcttcgatc accttcttgc
      901 taaaatcaat ttctttagca tgaaaaggca ccggcgtagt ggctatatcg ataaccaaaa
      961 tgtagttttc cttttcagca ataacacgga cagcctttaa tatttccggc atcagtttct
     1021 gaaacacttc ctgatcacgc ttttggagct ctgtattcgt gtcattgacc agcaattgat
     1081 aatcacgcag tttcttttgg taggcctctt ccttgtctct tcgcgcgttt tgcgtcataa
     1141 tcgagccttg tttgtccaac tcatccttca ttttctttaa ttcgttttct aaggatttaa
     1201 tgggtgccga tctcttatcg gcgaggcttt ttaggtcttc cgcagccttt ttaccggcga
     1261 tagaattttg cattatttcc cgtatgttaa taacaccaat tttttccgca gcaaaagcat
     1321 tagaactcca gacaaaaagc agcaggacca atcctgcaat tagataaata tttcttttca
     1381 ttttaaatct cctctctata ttattagcgg ataaagaaag acggaatctc ggttcatcca
     1441 cttttcggtt acataaacat gccgatggtg aattcccaac ggccgataga atcatcgttt
     1501 aatcctttgc ggtcaagtac gtagccatat tctaacctta aaggaccgat gggtgaatac
     1561 cagcggatac caagtcccac agtttgccgc aaatcatcaa tgtaatagcc gccattccag
     1621 gtatttccgg catcgtaaaa cactactcct ttcatgccgg catctttaat cagaggaaaa
     1681 acaagttcaa aattgaagtt tagcatagtt gtaccgccaa taacatcaga tgttcccgga
     1741 ttagtcgggc cgacgtaacg caaaccgcgc agagaattaa ttccgcctaa aacatatctt
     1801 tcgtaaatag ggatttttga atcgtcgaca tcattgtttt ggagaaatcc aatgcgcccc
     1861 ctcgctccga agacagtgtc ccaaatcaac ggataataag cggctaagct tgctccgtat
     1921 ttagtaagat taacatttcc tcccaaaggg tcgcctgcat gttcaacgga gatacttgat
     1981 ttggttccct tggaaggaaa tatataatca tctgttgtat cgcgtgttaa agttaatgtt
     2041 atagcgctgg taatcctctc gccagcttga gcaataatat aggaagaagc cgtggcgtaa
     2101 ttaatatctt taatatcatc aaaggaaagt ttatagccaa tgtagccggt gattttttcc
     2161 caaagagggt agcccaaagt aaatcctgcg ccacgcgtat ctaatgtgta agaatcatat
     2221 agctgtgtgt acttccagat atccactttg caccaaaggg gaagatcaaa aagccaaggt
     2281 tcagtgaaag ataaatctat gctgctactg gtggcactaa tacctccttt gaggcttaat
     2341 gtttgaccat atcccaagaa attttgttga acgatttgtg ccataatcag ggcctggtcg
     2401 gtggcgctat agccggcgcc caccataaac attcccgtat ttttttcttt gactcgaata
     2461 ttaatatcca ttttac
//
LOCUS       DASVIM010000077         4296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1478874, whole genome shotgun sequence.
ACCESSION   DASVIM010000077 DASVIM010000000
VERSION     DASVIM010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478874"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /gene="pdxT"
                     /locus_tag="VF305_01540"
     CDS             complement(<1..560)
                     /gene="pdxT"
                     /locus_tag="VF305_01540"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX7415848.1"
                     /translation="MALHLIKNKAAAIGVLDLQGGVHEHLDHLHRLGIANKRVKQETD
                     FADLAGLIIPGGESSCISRLLDIFGIKKILLQNYHRGMKIWGTCAGAILLAKKVINDE
                     SCLGLIDMEIERNSFGSQLDSFSSEALIPAVAAEPIPLTFIRAPKITKVGKDVRVLLK
                     IDGYIAAAENENILVTVFHPELTGCLA"
     gene            complement(565..1446)
                     /gene="pdxS"
                     /locus_tag="VF305_01545"
     CDS             complement(565..1446)
                     /gene="pdxS"
                     /locus_tag="VF305_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX7415849.1"
                     /translation="MDAKRHELNIQLAQMLKGGVIMDVTNVEQAKLAEEAGAVAVMAL
                     ERVPADIRKQGGVARMSDPAMIAEIKKAVKIPVMAKARIGHFVEAQILEALRIDYIDE
                     SEVLTPADEECHIDKTKFSIPFVCGARNLGEALRRIAEGAAMIRTKGEAGTGNVVEAV
                     RHMRTMNREIRQLTQMPLEEVTGFAKTNGMPYELALKVKELGRLPVVNFAAGGLATPA
                     DAALMMQLGCDGIFVGSGIFKSSDPAIRARAIVKATAYFNDPKKILEASMGLGEAMPG
                     LEISQIAPDQLLAGRGW"
     gene            complement(1589..2368)
                     /gene="hisF"
                     /locus_tag="VF305_01550"
     CDS             complement(1589..2368)
                     /gene="hisF"
                     /locus_tag="VF305_01550"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX7415850.1"
                     /translation="MLSKRIIPCLDVRDGKLTKGIKFKGNVDIGDPVEAAREYYEQGA
                     DEIVFYDITASSEGRDIMIDVVKRVAQTIFIPFSVGGGIRNLEDMRRVILAGAEKVSV
                     NSAAVTDPRIITQGAQAFGSQCIVLGMDVKKVDVSQKNPSGYEIVIHGGRKFTGIDAL
                     WWAKEAEKLGAGEICLNSIDADGTQNGYELNLTALISKNVNIPVIASGGAGKPEHLAE
                     VLTEGKADAALIASMVHYRTYTIAGIKKYLNEHQIKTRLYW"
     gene            complement(2530..3162)
                     /gene="hisH"
                     /locus_tag="VF305_01555"
     CDS             complement(2530..3162)
                     /gene="hisH"
                     /locus_tag="VF305_01555"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX7415851.1"
                     /translation="MMIAIIDYKAGNITSVARALQNIGETYVVTDDIRKLDNASHVIF
                     PGVGAAGEAMAYLRRKKLDSWLTNWIKTGKPLLGICLGTQIILDYSEENETNCIGLVR
                     GSTKRFPDDLNIACCKLKIPHMGWNNVTLHRDHPVFNDVPKDAEFYFVHSYYPAPADN
                     SLVLGVTDYGITFCSVLATKNLVAVQFHPEKSGRPGLQILRNFCRWRGNA"
     gene            complement(3244..4179)
                     /locus_tag="VF305_01560"
     CDS             complement(3244..4179)
                     /locus_tag="VF305_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418923.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEX7415852.1"
                     /translation="MFFYGIDCKNNKRYYDLKSYWRNLFGCNVHKLSIDAGFTCPNRD
                     GHVAVGGCIYCDGRGSKLRQKGELPSVIAQIQSGKNFYKPHASKFIAYFQTFTNTYAP
                     VAKLQALYAEALSQEDVIGLSIGTRPDCLAPETIELLSEYSKKYHIWLELGLQSIHDK
                     TLQIINRGHNFQQFLDTVHACAGRGINLCVHIIIGLPGESDEDILKTVKTLAGLPING
                     IKIHSLLALEGTALGKMYKNESIKLLTKEKYVSLVADVLEVLPPEMVIQRLTADGYRD
                     IFLAPSWAMNKLDVLNSINKELERRDSYQGICYKE"
BASE COUNT         1116 a         1041 c          887 g         1252 t
ORIGIN      
        1 gcaaggcatc cggttaattc cgggtgaaat accgtaacca gaatattttc gttttccgcc
       61 gccgctatgt agccatcaat ttttaaaagg acccggacat ctttaccaac cttagtgatt
      121 ttcggcgcac ggatgaaagt aagcggaatt ggttctgcag cgacagcggg aatcaacgct
      181 tcactggaga agctatctag ttggctgccg aagctgttgc gctcaatttc catatcgatc
      241 agaccaagac aagactcgtc gttgataact tttttggcta gcaaaatagc tcccgcgcaa
      301 gtaccccaga ttttcatgcc tctgtggtaa ttttgaagta aaattttttt aattccgaag
      361 atatcgagta acctggaaat acaggagctt tcaccgccgg ggataatcag tcccgccaaa
      421 tccgcgaaat cagtttcctg ttttactctc ttattggcga ttcccaatcg gtgcaaatgg
      481 tctaaatgtt catggactcc gccttgtaag tccaaaactc caattgccgc ggctttattc
      541 tttatcagat gcaaggccat atttttacca gccgcgtccg gctaaaagtt ggtctggcgc
      601 aatttgggat atttccaggc cgggcatggc ttcacccaaa cccatcgaag cttccaatat
      661 ctttttcgga tcgttgaaat aagccgttgc cttgactatc gctcgtgctc taattgccgg
      721 atcagaggat ttgaaaatgc cggaaccgac aaaaattcca tcgcagccaa gctgcatcat
      781 taaagcagcg tcagctggag tagcgaggcc acccgcggca aaattaacaa caggaagacg
      841 gcccaactcc ttgactttaa gcgccagttc gtaaggcatg ccgttggttt tagcaaaacc
      901 ggttacctct tccagcggca tctgagttaa ctgacggatt tcgcggttca tggttctcat
      961 gtggcggaca gcctcgacaa cgtttcctgt tcccgcctct cctttagttc ggatcattgc
     1021 cgcgccttcg gctatccggc gcaacgcctc acctaaattg cgcgcgccgc aaacaaaagg
     1081 aatggaaaat tttgttttat caatatggca ttcttcatca gcgggagtca gcacttcgct
     1141 ttcatcaatg tagtcaatcc gcaatgcttc caaaatttgc gcttcgacaa aatgcccgat
     1201 gcgtgctttg gccatgactg gaattttcac agctttttta atttccgcaa tcatggccgg
     1261 atcggacatt ctggccacgc ccccctgctt gcggatatcc gcaggcacgc gctccaacgc
     1321 cattacggct acagcgcccg cctcttcagc aagcttggcc tgttcgacat tggttacatc
     1381 cataatgacg ccgcctttta acatttgcgc caactggata tttaattcat gtctttttgc
     1441 atccacctta aattgcctcc taacttaaca attaaaatat taatacttaa tagcggtttt
     1501 gtatagtgaa atatttgtcc ctattggagt aaatttttct aattctaaaa aaacaacggg
     1561 aatttagtca agcatattca ttatcacact accaataaag ccttgtttta atttgatgtt
     1621 cgttcagata ttttttgatt ccggcaatcg tataggtgcg gtaatgcacc atggaagcaa
     1681 tcagcgccgc gtcggccttt ccttcggtta atacttccgc gaggtgttcg ggttttcctg
     1741 ctcctcccga cgcgataacg ggaatattca catttttgga aataagtgct gttaaattca
     1801 attcatagcc gttttgggta ccgtcggcgt caatggaatt caaacaaatt tcaccggcac
     1861 cgagtttttc cgcttctttc gcccaccaga gcgcatcaat accggtgaac tttcttccgc
     1921 catgaatgac tatttcgtaa ccggaaggat ttttttgaga aacatcaact tttttcacat
     1981 ccatacccag aactatacac tggctgccga atgcctgcgc tccttgagtg ataattcttg
     2041 gatcggttac ggccgcggag ttaacgctga ctttttctgc acccgccaga ataacccggc
     2101 gcatatcttc cagattgcgg atgccaccgc ccacagaaaa tggaatgaat atcgtttgcg
     2161 cgacacgctt gactacgtca atcataatat cgcgtccttc agacgaagct gtaatatcat
     2221 aaaagacaat ttcatcggcg ccctgctcgt aatattcccg tgccgcttcc acaggatcgc
     2281 caatatctac gttgcccttg aatttaatgc ccttggtgag tttgccatca cgcacatcca
     2341 ggcaaggaat gattcttttg ctaagcatga cggcctcgca aaaagtccat atgctgcgct
     2401 gcgctgcgtc cctcgttgtt gcggcgtacg ccaaaagtac gcctcacgcc tcgtgccttg
     2461 cgcgccttgc ctctggcgct ttttgcaatg tcgtccgtga ttacacgtac tttgattttc
     2521 ataacttcat taagcatttc ccctccagcg gcagaaattg cgcaagattt gcaaaccggg
     2581 gcggccgctt ttttccggat gaaattgcac ggcaactaaa ttcttcgtgg cgagaacaga
     2641 gcaaaaggtt attccgtaat cagtaacacc taaaaccagt gagttgtcag ccggagccgg
     2701 atagtaagag tggacgaaat aaaattctgc gtccttaggc acatcattaa ataccggatg
     2761 atcgcggtgc aacgtcacat tgttccagcc catatgagga atttttaatt tacagcaggc
     2821 aatatttaaa tcatccggga aacgctttgt tgaaccccgg acaagtccga tacaattagt
     2881 ctcgttttcc tcactgtagt caagaataat ttgagtgccc agacaaatac ccaacaatgg
     2941 cttaccggtc tttatccagt ttgtaagcca gctatccaat ttctttcggc gcagatatgc
     3001 catcgcttca ccggccgcgc cgactcccgg aaatattaca tggctggcat tatcaagctt
     3061 ccttatatcg tccgtcacca cgtaagtttc gccgatgttt tgtagagcac gtgcaacgct
     3121 cgtaatattt cctgctttat aatcaataat tgctatcatc attcattctt ggttttcttt
     3181 tttctcttac tgggcattta gcaattttta tgctcaaaat acaacagcta atttaaatta
     3241 gccttattct ttatagcaga tgccctggta cgaatccctt cgttctaatt ctttattaat
     3301 cgagtttaat acatctaatt tattcattgc ccaggaaggt gcaagaaaaa tatcgcgata
     3361 gccgtcggca gtcaggcgtt gaatgaccat ttcgggcggt aaaacttcca gaacatcagc
     3421 cactaaagaa acgtattttt ctttagttag aagtttaatt gattcatttt tgtacatttt
     3481 gcccagagcg gttccttcca gagccagcag tgaatgaatt tttataccat tgattggcaa
     3541 gcccgccaga gtctttactg tttttaatat atcttcgtca ctttcacccg gcaagccgat
     3601 aataatgtga acacagagat taataccgcg accggcacag gcatgaacgg tatctaaaaa
     3661 ctgctggaaa ttatgtccgc ggttgatgat ctgtaatgtt ttatcgtgaa tggattgcaa
     3721 tcccagctcc aaccaaatat gatatttttt tgaatattcg cttaataatt ctattgtttc
     3781 cggcgcgagg caatccggcc gagtgccgat ggagaggccg atgacatcct cttgacttaa
     3841 agcttcagca tagagtgcct gcaatttagc cacaggcgcg taagtattcg tgaatgtttg
     3901 gaaataagcg ataaatttgg aagcgtgagg tttataaaaa ttttttccgc tctgaatttg
     3961 tgctataact gagggcagtt cgcccttctg acgcagtttg gaacctcgcc catcacaata
     4021 aatacaacct cccacggcaa catgcccatc acgattaggg caggtaaatc ctgcgtcaat
     4081 ggataatttg tgcacattac agccgaacaa attacgccag taacttttca gatcgtaata
     4141 ccttttattg tttttgcagt ctatgccgta aaaaaacatc aattgaatta accctgagcg
     4201 agttcaacgc gttgatgggt ttattgatag tgtttttact attgattgaa tgcaagtgca
     4261 agtgtttggt aagggagaaa aaagttgtct gataat
//
LOCUS       DASVIM010000078         3804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_370300, whole genome shotgun sequence.
ACCESSION   DASVIM010000078 DASVIM010000000
VERSION     DASVIM010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370300"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2430
                     /locus_tag="VF305_01565"
     CDS             <1..2430
                     /locus_tag="VF305_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7415853.1"
                     /translation="FEGSHAIVMTSDLEPGKLFLALKGSGQCIYIGISSDQYMFSSEL
                     YGLVEVMPNFIKMSGELNVASENSAAGQIFILDQNSSGGLAGVVACYYDGTRINLKES
                     DIQKAEITTRDIDRGDFPHFFLKEISESAGSIKRTLRGKYRIVIKENSLPQVLFNLGE
                     DIIPASIRVGFQNSSIKNIMVIGHGTAAVAGAAVADALERYLKNKGINIMARVASELS
                     GFLLKDDLADTLVIPITQSGTTTDTNRAVAMAKERGAYVISIVNRRQSDITTKSHGVF
                     YTSDGRDIEMSVASTKAFYSQIIAGHVLALFFAQLLGARKDDYIAEELLNLEAAPKLM
                     AQVFARRDQISKSVTKTLAKKFWAIVGSGPNKAAADEIRIKLSELCYKTISSDIVENK
                     KHIDLSAEPLILVCAAGNPGPVVEDIVKDVAIFKAHKSAVVVFAEEGDDRFNKIADSV
                     ISIPASSMPMPVILNTMAGHLWGYYAACTIDKEALIFREFRNRLNLSMAEQVKGNYSL
                     YEGIADVHFRRMINEFYQPFNEQRNEGAFNLLSVKTISDLVLLLKYSAGKLPLEDFRH
                     EFNGDEGFASPLDLLDVTLGRAIDELTRPIDAIRHQAKTVTVGTSRKEKILKGIVFDL
                     LGKIKFTVKNLTYKNILAISRIQPAVSVVRGYTIYDINSLDEQGNPADSSTIAIRGKG
                     GVAKQMASRAETPALLMGTKRTIVSTGHVYIGRGKADGASIIILPLLGENRVVSNLLL
                     LHVDYNELLSLREKKEVLGYRYSDIRNLVNEYNINWDDHYLEKIPLADLFSEPVEVLA
                     GQIKQSAIAGK"
     gene            2634..>3804
                     /locus_tag="VF305_01570"
     CDS             2634..>3804
                     /locus_tag="VF305_01570"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX7415854.1"
                     /translation="MKTKFIFVTGGVLSSLGKGLAAASISALLECSGLKVTNQKLDPY
                     INVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTSTCMGKCNNLTTGQVYFSVITK
                     ERRGDYLGKTVQVIPHITNEIKDYIRQSAEGYDVSIVEIGGTVGDIESLPFLEAIRQF
                     RNEAGKQNAIFIHLTWVPFIKTAGEVKTKPTQHSVKALREIGIQPDILLCRTENFLSE
                     EIKSKIALFCNVEVAAVFTAKDVSCIYEVPLVFHREGLDSKIIDLLNIWTGQPRLDAW
                     EEVVNKFNNPLHEVCVAIVGKYVNLTDSYKSLNEALVHGGIANNCRVKLKFVDSEKIE
                     EEGIGDYLDDVDAVLVPGGFGSRGIEGMILAAQYARENKIPYFGICLGMQMAVVEY"
BASE COUNT         1077 a          722 c          906 g         1099 t
ORIGIN      
        1 tttgagggat ctcatgccat agttatgacc agtgatctgg aaccaggtaa attatttttg
       61 gcgctcaaag gcagcggcca gtgcatttat attgggataa gctccgatca gtatatgttt
      121 tcttcggaac tttatggtct ggtggaagtg atgcctaatt ttataaaaat gagcggcgag
      181 ttaaatgttg ccagtgaaaa ctcagcagcg gggcaaattt ttattttaga ccaaaattct
      241 tccggtggat tggcgggagt tgtcgcttgt tattatgacg gcacaagaat taatttgaaa
      301 gaaagtgaca tacaaaaagc cgaaataacg actcgagaca ttgatcgcgg cgatttccca
      361 cattttttcc tcaaagaaat ctccgaatcc gctggatcga taaagagaac cttgcggggt
      421 aaatatcgta ttgtaattaa ggaaaattct ttaccacaag tcttattcaa tttaggtgaa
      481 gatataattc ctgcttctat tcgcgtaggc ttccaaaata gttctattaa aaatataatg
      541 gttatcggcc atggaacggc agcagttgcc ggagccgccg ttgctgatgc tttggagcgt
      601 tacttaaaaa acaaaggtat caatataatg gccagagtgg cttccgaatt gagcggcttt
      661 ttgctgaagg acgatctggc cgacacctta gtgattccca taactcaatc cggaacaaca
      721 acagatacta atcgtgccgt ggcgatggcc aaagaacgcg gcgcttacgt aatttccatc
      781 gtcaacagaa gacaatcgga tatcacgact aaatcgcatg gagtatttta tacaagcgac
      841 ggacgcgata ttgaaatgtc ggtagcttct acgaaagcct tttactctca aattattgcc
      901 ggccatgttt tagccctttt ctttgcacaa cttttaggtg ctcggaaaga tgattatatt
      961 gccgaggaat tgcttaattt agaggcagcg ccgaaactga tggcgcaagt ctttgctcgc
     1021 agagatcaaa tttctaaatc ggtgacaaaa actctagcca agaaattttg ggcaatagtg
     1081 ggtagcggtc ccaacaaggc agcagccgat gaaattcgaa tcaagctaag tgaactatgt
     1141 tataaaacta tttcttccga tattgtggaa aataaaaaac atatcgattt atcggcagag
     1201 cctttaatct tggtttgtgc tgccggcaat ccgggaccgg ttgtggaaga catcgttaaa
     1261 gatgtagcga tttttaaagc gcacaagtcc gcagttgttg tttttgcgga agaaggggat
     1321 gatcgtttca ataaaattgc cgattccgtg atttccattc ctgcttcatc tatgcctatg
     1381 ccggtgattc tcaatacgat ggcggggcat ttgtggggct attatgctgc ttgcactata
     1441 gataaagaag cgcttatttt tagagaattc agaaaccggc ttaatttgtc tatggctgaa
     1501 caagtgaaag gcaactattc actttacgaa ggaatcgctg atgttcactt ccgccgcatg
     1561 atcaatgaat tttatcaacc tttcaatgaa cagcgtaatg agggtgcctt taatttacta
     1621 agtgtcaaga caatttccga tttggtgctt ttgttaaaat attctgccgg taaattgccc
     1681 ctggaagatt ttcgccatga attcaatggt gatgaaggtt ttgcctcacc acttgatctc
     1741 ttggatgtaa cgctggggag agctatagat gagttgaccc ggccaataga cgcgattcgc
     1801 catcaagcca agacggttac agtaggcaca agccgcaaag aaaaaatact taaaggaata
     1861 gtttttgatc ttctggggaa gattaagttt actgtgaaaa atttaactta caaaaatatt
     1921 ttggctataa gcagaataca gccggcggtt tctgttgtgc ggggatatac gatttatgat
     1981 attaacagtc tcgatgagca aggtaatccc gctgatagtt caactattgc catccgggga
     2041 aaaggcgggg tggccaaaca aatggcttcg cgcgctgaaa ctcccgcatt gctgatggga
     2101 acaaaacgaa cgattgtcag cactggtcac gtttatatag gtcgcggcaa agccgacggc
     2161 gcttctataa taattttgcc attattggga gaaaatagag ttgtcagtaa tttgcttttg
     2221 ctccatgttg attacaatga acttttgtca ttgcgtgaaa agaaggaagt cttgggttat
     2281 agatacagcg acattcgaaa cctggtcaat gaatataata ttaattggga tgaccattat
     2341 ttggaaaaaa taccgctggc tgatttgttc agtgagccgg tcgaagttct ggccgggcaa
     2401 attaagcaaa gcgctattgc cgggaaatga agttctgccg caagtttgca gggggagtgc
     2461 gggagctgtt gcagttacta ataccatttc gctttctttg gctaactttg ttggcagcgt
     2521 cgtcggcttc ctcaacgtat tagtaatacg tctgcggagc cttctccttg ccgcctcgtt
     2581 atcctttgaa agcgaaatgg tataatatac atggagaatg ttgaggaact atcatgaaaa
     2641 ctaaatttat ctttgttacc ggcggtgttc tttcttcgct gggtaaaggg ttggcagcgg
     2701 cctcaatttc agctttactg gaatgcagtg ggctgaaagt taccaatcaa aagctggatc
     2761 catatatcaa tgttgatccc ggaacaatga gcccttttca gcatggtgaa gtttttgtta
     2821 ccgatgacgg tgcggaaact gacctggatc tgggacatta tgaaagattt acctccacct
     2881 gcatggggaa gtgcaacaac cttaccaccg gccaggttta tttttctgtg attactaagg
     2941 aaaggcgcgg tgattattta ggcaagaccg tgcaagtaat tccccacatc accaatgaaa
     3001 tcaaagatta tatccgtcag tcggctgaag gatacgatgt ttctattgtg gaaattggtg
     3061 gcacggtcgg cgatatcgaa agtcttccat ttttggaagc tattcgccaa tttcgtaatg
     3121 aagcgggaaa acaaaatgcc atctttattc atttaacctg ggttcctttt attaaaactg
     3181 ccggcgaggt taaaaccaaa cctacgcagc acagcgtcaa agctctgcgc gaaataggta
     3241 tccagccgga tatacttctt tgccgaacgg agaattttct ttccgaagaa atcaaatcca
     3301 aaattgcgct tttttgtaat gtagaagtgg cggcagtttt tacggctaaa gatgttagtt
     3361 gcatttacga agtgccgctt gtttttcatc gcgaaggtct ggacagcaaa attattgatt
     3421 tgctcaatat ctggactgga cagccgcgtt tggatgcctg ggaagaagtg gtaaacaagt
     3481 tcaataatcc gctgcatgaa gtatgcgtgg ctattgtcgg taaatatgtg aatctcaccg
     3541 attcatacaa aagccttaat gaggcccttg tgcatggagg tattgctaac aattgccgcg
     3601 ttaaattaaa atttgttgat tcggaaaaaa ttgaagaaga gggcatcggc gattatttag
     3661 acgatgttga tgccgtttta gtccccggag gatttggcag ccgcggtatt gaagggatga
     3721 tccttgctgc gcaatatgca cgggaaaaca aaattcctta ttttggaatt tgtttgggga
     3781 tgcaaatggc tgtggtggaa tatg
//
LOCUS       DASVIM010000079         4465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1394370, whole genome shotgun sequence.
ACCESSION   DASVIM010000079 DASVIM010000000
VERSION     DASVIM010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394370"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..243)
                     /gene="rpmD"
                     /locus_tag="VF305_01575"
     CDS             complement(64..243)
                     /gene="rpmD"
                     /locus_tag="VF305_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX7415855.1"
                     /translation="MLKIKKVKSEIGRPEKQRKVLKGMGLIKLNDTVTLADNPQTRGM
                     INKVVHLVSVEESKE"
     gene            complement(291..788)
                     /gene="rpsE"
                     /locus_tag="VF305_01580"
     CDS             complement(291..788)
                     /gene="rpsE"
                     /locus_tag="VF305_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003133760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX7415856.1"
                     /translation="MIKEESLDKKEFKDVELLDRVVAINRTAKVVKGGRRFRFSAVVV
                     VGDGKGMIGTGLGKAHEVPEAIRKGMDMAKKNMVKVAVAGRTIPYEVIGNFGAGKVLL
                     KPASEGTGLIAGGAVRAVLEVAGVHNILTKCLGSHNPHNLVKATIEGLCQLKDPMEIA
                     AQRGK"
     gene            complement(808..1173)
                     /gene="rplR"
                     /locus_tag="VF305_01585"
     CDS             complement(808..1173)
                     /gene="rplR"
                     /locus_tag="VF305_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX7415857.1"
                     /translation="MASKKTLKIREKREKRVRGKIIGTEERPRLSVFRSDKHIYVQAI
                     NDTKASTVATASTLCEELAEKIKGKKKVDVAKEVGRLIAKRLQSLGVDKVVFDRGRFL
                     YHGRVKALAEAARENGLKF"
     gene            complement(1217..1756)
                     /gene="rplF"
                     /locus_tag="VF305_01590"
     CDS             complement(1217..1756)
                     /gene="rplF"
                     /locus_tag="VF305_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX7415858.1"
                     /translation="MSRIGKKPITFPKNVKISQSVGIVKVEGPKGILSSKLPEGITLA
                     IGDGSMVVERKSEERIVRSYHGLARTLIGNMVSGVSVGFEKNLEISGVGYRAEVAGKS
                     LKLALGFSSPVQYEIPKGIDIKVDKQVNIAVSGIDKQLVGRVAAEIRALKKPEPYKGK
                     GIKYAGEYIKRKAGKSASA"
     gene            complement(1792..2193)
                     /gene="rpsH"
                     /locus_tag="VF305_01595"
     CDS             complement(1792..2193)
                     /gene="rpsH"
                     /locus_tag="VF305_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX7415859.1"
                     /translation="MGMTDPIADMLTRIRNANKARFKSVNVYMSQMNVNIAKVLKKAG
                     YINSYDNVKDEKGQQMLKIALKYPDTKRAVITDIKRISKPGRRVYVASDSIPKVLNGY
                     GISVLSTSRGVITDEEARELNVGGEILCKVW"
     gene            complement(2216..2401)
                     /locus_tag="VF305_01600"
     CDS             complement(2216..2401)
                     /locus_tag="VF305_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX7415860.1"
                     /translation="MAKKSLIVKAKRKMKFSVREYNRCPLCGRPRAYYRKFDMCRICL
                     RKLSLQGELPGVIKSSW"
     gene            complement(2428..2967)
                     /gene="rplE"
                     /locus_tag="VF305_01605"
     CDS             complement(2428..2967)
                     /gene="rplE"
                     /locus_tag="VF305_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX7415861.1"
                     /translation="MARLKDYYSKTVVPALIKEFNYKNPMQVPKLGKIVINMGLGAAI
                     ENVKIIDGAVQELAMITGQKPVITKAKKSIATFKLRQGMSIGCSVTLRKEMMYEFFDR
                     LVNVALPKVRDFRGISPNAFDGRGNFSMGLQEQIIFPEIEYDKVEKIKGMNITIVTTA
                     KSDAEARALLKLMGVPFKN"
     gene            complement(2974..3297)
                     /gene="rplX"
                     /locus_tag="VF305_01610"
     CDS             complement(2974..3297)
                     /gene="rplX"
                     /locus_tag="VF305_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX7415862.1"
                     /translation="MSLNKFKKGDMVKVLTGKDKGKTGKILKSIPDKGRIVIEKINII
                     KKHKRPDQKTKGGVVEKEGSVHISKVGLLCNKCNTAVRIKSKILEDGKKVRICSKCND
                     VINVG"
     gene            complement(3317..3685)
                     /gene="rplN"
                     /locus_tag="VF305_01615"
     CDS             complement(3317..3685)
                     /gene="rplN"
                     /locus_tag="VF305_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX7415863.1"
                     /translation="MIQMQTILNVADNSGAKKVACIKVLGGSKRRYASLGDVIVVSVK
                     EAIPNSKVKKGDVMKAVIVRTIKEVKRADGSYLKFDDNSAVLISNQLEPIGTRIFGPV
                     ARELRAKQFMKIISLAPEVL"
     gene            complement(3728..3985)
                     /gene="rpsQ"
                     /locus_tag="VF305_01620"
     CDS             complement(3728..3985)
                     /gene="rpsQ"
                     /locus_tag="VF305_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX7415864.1"
                     /translation="MAVQGNKRTIKGVVISDKMDKTIVIRAERLVKHPVFHKYLRKHM
                     KYKAHDEQNQCKVGDMVLIIEARPLSKEKRWRMLEILEKAK"
     gene            complement(3987..4181)
                     /gene="rpmC"
                     /locus_tag="VF305_01625"
     CDS             complement(3987..4181)
                     /gene="rpmC"
                     /locus_tag="VF305_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX7415865.1"
                     /translation="MKIKEIRDLGINELKQKNGEILEELFRLKIRHASGQLESPAMLG
                     RLRKDIARINTVLKEKEAVI"
     gene            complement(4171..>4465)
                     /gene="rplP"
                     /locus_tag="VF305_01630"
     CDS             complement(4171..>4465)
                     /gene="rplP"
                     /locus_tag="VF305_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX7415866.1"
                     /translation="ARQIEAARIAMTRHVKRGGKIWIRVFPHKSVTKKPAETRMGKGK
                     GAPEGWVAVVKPGSVLYEMEGVTREVAKEAFRLAAHKLPIATKFLARDISDEN"
BASE COUNT         1137 a         1169 c          686 g         1473 t
ORIGIN      
        1 acccactcgc tttctctttt tcgtagcacc ggccggagcc ttaagagaac tcaaatccat
       61 atactactcc ttcgactctt ccacggaaac taaatggaca actttattta tcataccacg
      121 tgtttgcgga ttatcggcta aggttacagt gtcatttaac ttgatcaatc ccattccttt
      181 taagactttt cgttgctttt cgggacggcc gatttcgctt ttcacctttt taatttttaa
      241 cattttaccc acttccagct acctcatcta acatcaaata attgataaga ctacttgccc
      301 ctttgcgcgg caatttccat tggatctttc aactggcaaa gcccctcgat agtagcttta
      361 accaagttat gtggattatg agatccaaga cacttcgtca aaatattatg gacaccggca
      421 acttccaata ctgctcttac agcccctccg gcaatcaaac ccgttccttc agatgccggt
      481 tttaataaaa ctttccccgc accgaagttg cctatgactt cataaggaat cgttcttccc
      541 gcaacggcaa ctttcaccat attttttttg gccatgtcca ttcccttgcg gatagcttcc
      601 ggcacttcgt gagcctttcc taatcccgtt ccaatcatgc ccttgccatc gcctacaaca
      661 acgacagcgc taaaacgaaa ccgccttcct cccttgacca cttttgctgt tctgttaatg
      721 gcaacaactc gatcgaggag ctccacatct ttaaattctt ttttatccaa cgattcttcc
      781 tttatcatcg agtattattt acaaaaatta aaatttaaga ccattttccc gcgcagcttc
      841 tgctaatgcc tttacgcgac catgatagag aaatcttcct ctatcaaaaa cgactttatc
      901 cacacccaaa gactgcaagc gttttgcaat taatctgccc acttcctttg ccacatctac
      961 ttttttcttg cctttgatct tttcagctaa ctcctcgcac aaagtagatg ccgttgctac
     1021 agtactcgcc tttgtatcat tgatggcttg aacataaata tgtttatcac tacggaatac
     1081 ggaaagacga ggtctctcct cagtcccgat gatttttccc cttactcttt tttctctctt
     1141 ttccctgatc tttagtgtct ttttggaagc caatgttcca cctctttatg gcaacataaa
     1201 atttttactt gtttaattaa gcactggccg actttccagc tttgcgcttg atatactcgc
     1261 cagcatactt aatgccctta cctttataag gctctggctt cttcaacgcc ctgatttccg
     1321 ccgcaaccct tccaaccagt tgcttatcaa taccggaaac agctatatta acttgtttgt
     1381 ccaccttaat gtctattccc ttgggaattt cgtattgcac cggactggaa aaacctaaag
     1441 ccaactttaa gctctttcca gcaacctcgg cgcgataacc aacgccggat atttccaagt
     1501 ttttttcaaa accaacgctg acaccactta ccatgtttcc gattaaagtt ctggctaaac
     1561 catgataaga gcgcacaatt ctttcttcag attttctttc taccaccatg ctaccgtcac
     1621 ctattgccag agtaatgcct tccggcaatt tcgaagaaag aattcctttg ggcccttcca
     1681 ctttaacgat gccgacactt tggctgattt taacattttt gggaaaagtt atcggctttt
     1741 tacctattct cgacatgaac aagcgctcct gcttaatatc atttattcaa attaccaaac
     1801 tttacaaaga atttcgccac ctacattcag ctctctggct tcctcatcgg taataacgcc
     1861 gcgggaagtc gaaagaacag aaattccata accatttaga acttttggaa tactgtcaga
     1921 agcaacataa accctgcgtc ccggtttact aattctttta atatctgtaa ttactgcacg
     1981 tttagtgtcg ggatatttca gagcaatctt caacatctgc tgtccctttt catctttcac
     2041 attatcgtag ctgttaatat atccagcttt tttcagtacc ttggcaatgt tgacgttcat
     2101 ctgagacata taaacattga cactcttaaa acgagcctta ttagcattcc taattctagt
     2161 aagcatatcg gcaataggat cagtcatccc catgcttttt ctccttaacc ttaagttacc
     2221 aactcgattt gattacgcca ggaagctcac cttgcaggga aagtttccgc aaacaaattc
     2281 tacacatgtc aaattttctg taatacgctc gcggccggcc gcaaagcggg cagcggttat
     2341 attctctgac cgaaaacttc atctttcttt tcgcttttac aattaacgac ttcttggcca
     2401 caccaaattc ctccgaagct ttcttcctta attcttaaaa ggcacaccca tcagctttag
     2461 taaagctctt gcttcagcat cacttttagc cgtagttacg atggttatat tcattccctt
     2521 gatcttttcc accttatcgt actcaatctc cgggaaaata atttgttcct gcaaacccat
     2581 ggaaaaattt cccctgccat caaaagcatt gggtgatata ccgcgaaagt ctcttacctt
     2641 gggtagggct acattgacca accggtcaaa aaactcatac atcatttctt ttcttaacgt
     2701 tacggagcag ccgatggaca ttccctgcct caacttaaat gtcgctatcg atttcttggc
     2761 cttggttatt accggtttct gcccggtaat catagctaat tcctgaacgg caccatcaat
     2821 gatttttaca ttttcaatgg ctgcaccaag acccatatta attacaatct ttcccaactt
     2881 gggcacttgc atcggatttt tgtaattaaa ctccttaatc agagcaggaa ctactgtttt
     2941 tgaataataa tcctttagtc ttgccatttt cctctaaccc acattgatta cgtcattgca
     3001 tttgctgcag atgcgcactt ttttcccatc ttcaaggatc ttacttttta tcctcacagc
     3061 tgtattgcat ttattacaca acaatccaac tttagatata tgaactgatc cctctttttc
     3121 cactacacca cctttagttt tttgatccgg ccttttatgc ttcttaataa tattaatttt
     3181 ttctataact attcttccct tatcgggaat agatttcagt atcttaccgg ttttcccttt
     3241 atctttaccg gtaagaactt taaccatatc gcccttttta aatttattta aactcatttt
     3301 cttcctcgtc taacttttac agaacctctg gcgccagaga tataattttc ataaactgtt
     3361 tcgctctaag ttcacgagca acaggcccaa aaatccgtgt tccaatgggc tccaattggt
     3421 tggaaattaa aacagccgaa ttgtcatcaa attttagata agagccatcc gcccttttca
     3481 cttcctttat ggtacgcaca atgactgcct tcatcacatc gccttttttt actttagagt
     3541 taggaatcgc ttcctttact gaaacgacga ttacatcccc caaactcgca taacgacgct
     3601 ttgatccacc caacacttta atacaagcaa ccttcttggc gccggaatta tccgcaacat
     3661 ttaatatggt ctgcatttga atcataaaaa aactcccatc aaaacaactt aatcgccaaa
     3721 tagttcgcta ttttgctttc tccaaaattt cgagcatgcg ccagcgtttt tctttgctca
     3781 acggtcgtgc ctcaataatc agaaccatgt ctcccacttt gcactggttt tgttcatcat
     3841 gggccttgta tttcatatgt ttgcgcaaat atttatgaaa aacaggatgc ttcaccaaac
     3901 gttcggctct aatcacgatt gttttatcca ttttatcgct tatcactacg cccttgatcg
     3961 tgcgcttgtt tccttgaaca gccatgttag atcacagcct ccttttcctt cagcaccgtg
     4021 ttgattctgg ctatgtcctt gcggagacgc cccaacattg ccggcgattc cagctgaccg
     4081 gatgcatgcc gaatctttaa tcgaaaaagt tcttcgagaa tttcaccatt tttttgtttg
     4141 agctcgttga taccgagatc tctgatttcc ttaattttca tcagatatat cccttgctaa
     4201 aaacttagtg gcaataggta acttgtgggc ggccagacga aaggcttctt tggccacttc
     4261 cctggttaca ccttccattt catagagaac cgaaccaggt ttaactacgg ctacccatcc
     4321 ttcgggtgct ccctttccct tacccattcg agtttccgcg ggttttttag tcactgattt
     4381 gtgtgggaat accctgatcc aaattttacc accgcgcttc acatgacggg tcatcgcaat
     4441 acgcgcggct tcgatttgtc ttgca
//
LOCUS       DASVIM010000080         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1480418, whole genome shotgun sequence.
ACCESSION   DASVIM010000080 DASVIM010000000
VERSION     DASVIM010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480418"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..841)
                     /locus_tag="VF305_01635"
     CDS             complement(50..841)
                     /locus_tag="VF305_01635"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HEX7415867.1"
                     /translation="METTIYFYTGTGNSLWTAKKLSELLGGTALIPLTLANKGTINTS
                     AKRIGLVFPVHIWGMPSPVIDFVNRLVADPAKYYFAIAVNAGQVAATLIQLKRLMEAK
                     SLNLSSGFSIDLPSNYIPWGGAVSPEAQQKKFNAALIKFSRISASINAKEEIPPEKGP
                     AWQNIFLSLFYRQSFPHVPKMDKSFGVDEKCTSCAICEKICPAQNILITDGKPTWQHH
                     CEQCFACLQWCPEEAIQYGKNTAKKKRYHHPEIKLQDMLVCVPQK"
     gene            complement(851..1318)
                     /locus_tag="VF305_01640"
     CDS             complement(851..1318)
                     /locus_tag="VF305_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX7415868.1"
                     /translation="MTKYLLFLMTLVFVFYGMANADFYTWEDETGATHIADYPPPKAG
                     PAQKVEVHKDTTASPNDEEQVTSSKDTKSKSKKPEVTFYTKNDCSDCDKAREFLKSKN
                     ISFTEYNMDTDKTAAVKRKDIDGSEDVPFAIINRNQVYGFSESVYNRALQLAP"
     gene            1582..>2121
                     /locus_tag="VF305_01645"
     CDS             1582..>2121
                     /locus_tag="VF305_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7415869.1"
                     /translation="MLGRPLSLFKVFGFEIKVDISWLFLAALITWSLASGLFPEYYKD
                     LPPAAYWWMGAAGTVGLFLSIIAHELVHSLVARYYGIPMKGITLFIFGGVAQMEDEPP
                     NPKAEFMMAVVGPLSSFLIGIFSFLLYYLGEKSGWPVTVNGVLAYLAWLNTVLAVFNL
                     IPAFPLDGGRILRSALWNWK"
BASE COUNT          556 a          417 c          452 g          696 t
ORIGIN      
        1 ttccttaaga atcgaagatt ctctgcagct tgccgcggag agcttcaaat catttctgag
       61 gaacgcagac gagcatatct tgcagcttga tttcgggatg atggtagcgt tttttctttg
      121 ctgtattttt accgtattga atcgcttcct cggggcacca ttggagacag gcaaaacatt
      181 gttcgcaatg atgctgccat gttggtttac catcggtgat caatatattt tgggcgggac
      241 agattttctc gcaaatcgca caacttgtgc atttttcgtc aaccccaaag gatttgtcca
      301 tcttagggac atgagggaaa gattggcgat aaaaaagaga aagaaaaata ttttgccagg
      361 ctggaccttt ttcaggaggt atttcttctt tggcgttgat agatgcagaa atccggctaa
      421 attttataag agctgcgtta aattttttct gctgcgcctc tggagaaacg gcgccgcccc
      481 agggaatgta attgctaggc aagtcgatgg agaatcccga agaaagatta agactctttg
      541 cttccattaa tcttttcaat tgaattaaag tggcagcaac ctggccggca ttgacggcaa
      601 ttgcaaagta atattttgcc ggatcagcca ccagacggtt gacaaaatcg attactggcg
      661 aaggcattcc ccagatgtga acggggaaga ccagacctat acgttttgcg ctggtgttaa
      721 tagttccttt attggcaagt gtcagaggaa tcaacgcagt gccgcccaac aactctgaaa
      781 gcttttttgc cgtccaaagg gagtttcccg ttcctgtata aaaataaatt gtagtctcca
      841 tttgtctccc tcagggagca agttgcaaag cccgattgta aacgctttcc gaaaatccat
      901 atacttggtt tctgttgata attgcaaagg ggacatcttc actgccgtca atatcctttc
      961 tttttacagc agcagtctta tccgtgtcca tattgtattc agtaaaagaa atatttttgg
     1021 atttcaggaa ttcacgcgct ttatcgcaat cactgcaatc gtttttggta tagaaggtga
     1081 cttccggttt cttagacttt gatttggtgt ctttggacga tgttacctgt tcctcatcgt
     1141 ttggcgaggc tgtggtatcc ttatgaactt caaccttctg agctggtccg gcctttggag
     1201 gcggataatc tgctatatgc gtggctccgg tttcatcttc ccatgtataa aaatcggcat
     1261 ttgccattcc ataaaaaacg aaaaccaatg tcatcaaaaa cagtagatat tttgtcatca
     1321 atacctcccc cgcattaagt ctttttaaat tagcggcaaa aatattttag atttaaacat
     1381 aaaaaattac cttagtgtta ctattctgta aaaaactaat attttaataa aaatagctta
     1441 aaagcctcat ttttgtcaat ttaactggag gaattatttt atattacatt ttactggatt
     1501 tgtttataaa ttgcaattta atacattgat ttttgtcata tagggattat cttagtaaaa
     1561 aatatttaac agggagttat tatgcttggt cgtccattaa gtctttttaa agtctttggg
     1621 tttgaaataa aagttgatat tagttggctt tttttagcgg ctttaataac ttggtccttg
     1681 gccagcggcc ttttccccga atattacaaa gatctgcctc ctgccgccta ctggtggatg
     1741 ggagccgccg gtacagtcgg acttttttta tcaataattg ctcatgagct ggttcattcc
     1801 cttgtggcca gatactatgg aataccaatg aagggcataa ctctgttcat tttcggcggt
     1861 gtcgcacaga tggaggacga accccctaat cccaaagctg aatttatgat ggctgttgtt
     1921 ggtcctcttt ccagctttct tatcggtatt ttctcgtttt tactctacta tcttggagaa
     1981 aaaagcggct ggccggtgac agtcaatggc gttttagcct atttggcctg gttaaacacg
     2041 gttctggcgg tttttaattt gattcctgct tttccgctgg atggcggaag aatattacgt
     2101 tcagctcttt ggaattggaa a
//
LOCUS       DASVIM010000081         6344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_115162, whole genome shotgun sequence.
ACCESSION   DASVIM010000081 DASVIM010000000
VERSION     DASVIM010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115162"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VF305_01650"
     CDS             <1..711
                     /locus_tag="VF305_01650"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX7415870.1"
                     /translation="NDSGNLQDSGKKGKNTGHLMDRYKKQRSRMVETQIKSRGISDSR
                     VLRAMETVPRHLFIDEGLIDQAYNDNPLPISEKQTISQPYIVALMTQALELNGKERVL
                     EIGTGSGYQTAILASLAERIFSIERIAPLAIKARKILDRLNYYNVAIRVGDGSYGWKE
                     ESPFDAIITTAAAPEIPQYLVEQLAVGGRLVVPIGGRDVQTLYKLTRLIENPQEIRKE
                     DLGGCRFVSLIGESGWKE"
     gene            699..1649
                     /locus_tag="VF305_01655"
     CDS             699..1649
                     /locus_tag="VF305_01655"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7415871.1"
                     /translation="MERIKPVLASGIFILLALLFIISCSGLQVQEAPAKGVYHRVKKG
                     ETVWSIARAYHVKLQDLAEMNNITNLNLIEEGAVIFIPDANQVIDDVMAYVKTMDTAD
                     KITTKIDSPENIKNPVENNEGKEGKTATEAQTIMDKKVVPPMVTSEKLQPVKVAPNEE
                     YQPAEVIPAQKPPSEKIEPKPKVNLPIEEIRIEKRKFIWPVRGSVKTRFGIQPNKTYH
                     NWIKIVSTVGTPVKAAASGAVIFSSRLKNFGETVIIRHEDNFSTVYTHLKKRYVRTDQ
                     NVKKGEAIALVGETDGAGNAYINFEIRLQGKARNPLFFLP"
     gene            1720..2865
                     /locus_tag="VF305_01660"
     CDS             1720..2865
                     /locus_tag="VF305_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7415872.1"
                     /translation="MNNYFEQGPIRPPSEATSLLIRVTRNCPWNKCVFCHTYRGSKFE
                     LRSVEEVKKDIQAMKDIADQIVGLSWKLGEGGHVNRNVLTQIYNSNGFYNDFFQTVAV
                     WLYFGGENVFLQDANSLIIKTPDLLTILDFLREKFPQIKRITSYCRSHTAVRKTVGEF
                     EQLRKAGLSRIHIGMESGCDEVLSLIHKGVTANDHIKAGQNIRQAGIELSEYVMPGLG
                     GAKWTRQHALETARVINAINPDFVRLRTLHVVPGTELDELMRKGEFQPLGDEDILKEI
                     QIFIENLDVQGTKLASDHILNLLEELEGKLPADKPKILAAIKRYFELSVEDRLIFRLG
                     RHSGALRKLDDLADEETYLRLKSIVDRYAVEGGNLEEDMVKIMNNYI"
     gene            3400..5238
                     /gene="pilM"
                     /locus_tag="VF305_01665"
     CDS             3400..5238
                     /gene="pilM"
                     /locus_tag="VF305_01665"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX7415873.1"
                     /translation="MASLNDINSTEKLLNVIRGKNEEPFRTLGKPEVFLSKKKPAKKI
                     KITLPQHLPDKKRYTIGIDIGQEFIRLAKTTKASDGRPILVDQKVIYSHQASKGSPEF
                     NTLLKSSVIAFCGSIADCNIWTMMTAGEVNVYHIKIPHVQKKQLENVIYWTAKKENPI
                     DEKNFIFDFELQGEIIDQGIPKYSVMVYTVPKVEIEKVKTLFSDMGITLAGISIAPFA
                     IQNIFRSKWMSADEETFASLFIGNDFSRIDIYRKENLVMTRGIKTGTNSMMDAITDSV
                     LEKTGNLKLEKDEVKKILFSLGPDSEKLRDTDAGYDLKEEEILEMIFPAWERLARQIE
                     LTLKYYTSSVGYEKVEKLYVSSSMNVYNPVLIYMNDQLGTKTEFFDPFKQQPPSPANE
                     SISISERVSLVPALGLALSDNTRTPNAIFTYREKNRDIRVKRINRGIFSSFAAALIIC
                     IIALVYQGAEVSILSKQRVKLEKELALYNPILSTDKISKIADEVKTQRNISRQYAQRY
                     LGVAAIGEVTALTPQNIRLINFRITTGSGSPKDNTDKTAKETGDGVIIEGVILGESNV
                     LDSLLAQYVMKLESSPMLRQVSVQKSGNITFKKSEVLHFTLSAKIG"
     gene            5244..5780
                     /locus_tag="VF305_01670"
     CDS             5244..5780
                     /locus_tag="VF305_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415874.1"
                     /translation="MKKFNINIPERSLWYLIICGGIILLFVLAGIFPLYQYNSHRINE
                     NKKLKYQIEEQKELGPIYLTLLKIMENKDLRVLPNPKKATIPRVEAGKFQDAFRAIAG
                     KSGLMTISLTPDLTTLAESSTFLLHNAVLKGEFDNFRKMLIGLGAVPYLDRIEEIRIQ
                     QHPDSMEFRMKIWIALGS"
     gene            5797..6294
                     /locus_tag="VF305_01675"
     CDS             5797..6294
                     /locus_tag="VF305_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415875.1"
                     /translation="MTKLNKRQIIILVIAALFVLYAVYEYLIAGPASKKVKTSADPAE
                     INTFVSELTSNLIKDNLAAVDAYIVGRAEADWQKNPFWERESYKNWAAREGAAGGSGA
                     AAKIIYSGYVDSGNKKMAVINGLEYRIGEQLEMEGYVLKNITSSKVLIVNKNTGSEME
                     VSIQE"
BASE COUNT         2082 a         1127 c         1383 g         1752 t
ORIGIN      
        1 aatgattcag gaaatttaca ggattccggc aaaaaaggaa aaaatacagg tcatttaatg
       61 gatagatata aaaagcaacg gtcgcggatg gtggagacac aaataaaatc gcgtggcata
      121 agcgacagcc gggtgttgag ggctatggaa accgttccgc ggcatctttt tattgacgaa
      181 ggtctgattg atcaagcata caatgacaac cctctgccta tcagtgaaaa acaaacgatc
      241 tctcaacctt acattgttgc cctgatgaca caagcactgg aattaaacgg aaaagaaaga
      301 gtcttggaaa tcggtacggg gtccggttat cagacagcaa ttttggcttc tcttgccgaa
      361 cgcatttttt ccattgaacg tattgctcca ttggcgataa aagcgcgtaa aattcttgat
      421 cggctcaact attacaatgt ggccataaga gtgggtgatg gttcttacgg ttggaaagaa
      481 gaatcccctt ttgacgccat tatcaccaca gccgcagcgc cggaaattcc tcaatattta
      541 gtcgaacagc ttgccgttgg cggaagactg gttgttccca ttggtggccg tgatgtgcaa
      601 accttatata agctgacccg ccttatagaa aatccgcaag agataagaaa agaggactta
      661 ggcggatgcc gttttgtcag cctgatcggg gagagtggat ggaaagaata aaacctgtat
      721 tagcgtcagg tatttttatt ttactggctt tgttgttcat tatttcttgt tctggcctac
      781 aggtgcaaga agcgccggca aagggtgttt atcatcgggt gaaaaaaggc gaaacggttt
      841 ggagcattgc ccgcgcttat catgtcaaat tacaagattt agccgaaatg aataatatta
      901 caaatctcaa cttaattgaa gaaggcgcag ttatattcat tcccgatgcc aaccaagtaa
      961 ttgatgatgt tatggcctat gtaaaaacta tggatactgc ggacaagatc acaactaaaa
     1021 tagatagtcc cgagaatatt aaaaaccctg tggaaaataa tgaagggaaa gaaggaaaaa
     1081 ctgcgaccga agcgcagact attatggata aaaaagtggt gccgcctatg gtaacatccg
     1141 aaaaactaca accggtaaaa gttgctccta atgaagaata tcagccagcg gaagttattc
     1201 cggcacaaaa accgcctagc gaaaaaatcg agcccaagcc taaagtaaat ttgcctatag
     1261 aagaaatacg gatagaaaaa aggaagttta tttggccggt gcgcgggagc gtgaaaaccc
     1321 gtttcggcat ccagcccaac aagacttacc ataactggat taaaattgtt tccactgtag
     1381 gaacgccggt aaaggcagcg gcatccggcg ccgttatttt ttcttcccgg ttaaaaaatt
     1441 ttggagaaac agttattatc cgccatgaag acaatttttc cacagtctat acacacctga
     1501 agaaaagata cgtcaggacg gatcaaaatg tcaaaaaagg ggaagcgatt gccctggtag
     1561 gagaaacaga cggggcaggc aatgcctata taaattttga aatccgcttg cagggcaagg
     1621 cgcgcaatcc tctatttttc ctgccttaat ttttacttac tcttgccata aaacaagaag
     1681 tagttataat ataactttat gttgctctaa gggatgaaaa tgaataatta ttttgaacag
     1741 gggccgattc gtccgcccag cgaggcgaca agccttttaa ttcgtgtaac aagaaattgt
     1801 ccgtggaata aatgcgtctt ttgtcatacc taccgtggca gtaagtttga actaagaagc
     1861 gtggaagaag ttaaaaaaga tatccaggca atgaaagata ttgccgatca aattgtcggc
     1921 ctttcctgga aattgggaga aggtggccat gttaatagaa acgttttaac gcagatttac
     1981 aacagcaacg gtttttacaa tgattttttt caaacggtgg cagtgtggct ttactttggc
     2041 ggtgaaaatg tatttttgca ggatgccaac tctctaatta taaagacacc cgatctttta
     2101 actattcttg attttctacg ggaaaagttt ccccaaataa aaagaattac ttcttactgc
     2161 cgttcgcaca cggcagtaag aaagacagta ggtgagtttg aacaattaag aaaagctggt
     2221 ctttcccgca tccacattgg catggaaagc ggctgtgacg aagttttatc tttaattcac
     2281 aaaggtgtta cagccaatga ccatattaaa gccggacaga atatcagaca agcagggatt
     2341 gaactcagtg agtacgtaat gcccggatta ggcggagcaa aatggactcg tcaacatgcc
     2401 ttggagaccg ccagagttat aaacgcaatt aatcctgatt ttgttcgttt acgcacgctg
     2461 catgttgtac cgggcaccga gctggatgaa ttaatgagaa agggtgaatt ccagccgctg
     2521 ggtgatgaag atattcttaa agagatacaa atttttattg aaaatttgga cgttcaggga
     2581 actaagctgg ctagcgatca tattttaaat cttctggaag aattggaagg aaaacttccc
     2641 gcagataagc ccaagatttt agcggcgatt aaaaggtatt ttgaactttc tgttgaagac
     2701 cggcttattt ttcgcctggg ccgacacagc ggcgcattaa gaaaacttga tgatcttgcc
     2761 gacgaagaga catatttaag gttgaaatct atagttgatc gttacgccgt agaaggcggc
     2821 aacttagaag aagatatggt caaaataatg aataattata tttagaaaat gaaaaatatt
     2881 gaagattttg taagtacgtt gaggaagaaa taattttgcg taactgtcct aaattttgca
     2941 gcactcgttg tttattaagg gtggaaggag taatagtgag cgctaaatac tgcactatcc
     3001 taataagaag tgactccgct aaaagctacg ttcataaaaa agttcaaatt gatctttccc
     3061 cagtgcctaa atgcatttgg tgctttttta aatattaaaa gagcttcata cggctgctgt
     3121 ccgtgatgat tgtacaagaa taacttatgc ggaaacattt ttgattattt cgtgtttaat
     3181 cattctcctg gctcaagcaa atatccaatt tggaaattgt ctttttataa gctgcaaaaa
     3241 gttaccggat tatttgtccg gaaaaaccgt tcccatttct cgaaaaaatc aggcatgaaa
     3301 aaataaaaaa cccttataat gtcttgcatt aatttagaaa tattagtaaa ataaccatta
     3361 tttgaaaaaa taaccggttt ttttaaggag aatttatttt tggctagttt aaatgatatc
     3421 aactcaacag aaaaacttct taatgttata cgcggcaaaa atgaagaacc tttcaggaca
     3481 ttgggaaagc cagaagtttt tttgtctaaa aagaagccgg caaaaaaaat aaaaattacc
     3541 ttgcctcagc atcttcccga taaaaaacgt tacactatcg gaattgatat tggccaagaa
     3601 ttcattcgtc tggctaagac aactaaagct tctgacggca ggccgatttt agttgaccaa
     3661 aaagtcatct atagtcatca ggcatctaag ggctcacccg aattcaatac acttttaaaa
     3721 tcatctgtga ttgccttttg tggttcaatt gccgactgta atatctggac gatgatgacg
     3781 gcgggcgagg ttaatgtcta tcacattaaa attccacacg ttcaaaaaaa gcaattggaa
     3841 aatgtgatct attggacagc gaaaaaagaa aatcccattg atgaaaaaaa tttcattttt
     3901 gatttcgaac ttcaaggaga gattattgat caaggaattc ccaaatattc cgttatggtt
     3961 tacacagtgc ccaaagtgga aattgaaaaa gttaaaactt tattttcgga tatggggata
     4021 actcttgccg gaatttcaat tgctcctttc gctattcaaa atatttttcg atcaaagtgg
     4081 atgtcagcgg atgaagaaac ctttgccagt ttgtttatcg gcaatgattt ttcccgcatc
     4141 gacatttata gaaaagaaaa tctggtgatg acccgcggta taaagacggg caccaacagt
     4201 atgatggatg caattaccga ctctgtttta gaaaaaaccg gcaatttaaa gctggaaaag
     4261 gatgaagtaa aaaaaattct tttcagcctt ggccctgatt cagaaaaatt aagagataca
     4321 gatgccggtt acgacttaaa agaagaagaa atattggaaa tgatcttccc agcatgggaa
     4381 agattagccc ggcagattga gctgacatta aaatattaca catcttcggt cggctacgaa
     4441 aaagttgaaa agctctacgt ttcatcgtcg atgaatgttt ataatcctgt tctgatttac
     4501 atgaatgatc agttagggac aaaaacagaa ttttttgatc cttttaaaca acagccgcct
     4561 tcccctgcta acgagtctat aagcatttcc gagagagtgt cattggttcc cgctttgggt
     4621 cttgccttgt cggataatac gcgcacgcct aatgctattt ttacttatcg cgaaaaaaat
     4681 agagacatac gtgtaaaacg gattaataga gggatttttt cttcctttgc ggccgcttta
     4741 attatttgca tcatcgcgct cgtctaccag ggtgcggaag taagtatttt aagcaaacaa
     4801 cgggtaaaat tagaaaaaga attagccctt tataatccga tattatcgac ggataaaatc
     4861 tctaaaatag ctgacgaagt aaagactcaa agaaatattt cccgtcaata tgctcaaagg
     4921 tatctggggg tcgcggcgat tggagaagtt acggctctta caccccaaaa tatacgtttg
     4981 atcaatttta gaataactac gggaagcggt tcgccaaagg ataatactga taaaacagca
     5041 aaagaaacag gtgatggtgt tataatagag ggagttattt taggggaaag caatgtgctg
     5101 gattcattgt tggcgcaata cgtaatgaag ctggagagtt caccaatgtt gcgtcaggta
     5161 tcggtacaaa aaagcggcaa tatcactttc aaaaaaagtg aagtccttca ttttactttg
     5221 agcgctaaaa taggttagct ctgatgaaga aatttaatat taatataccc gaaagaagtc
     5281 tttggtacct aataatttgc ggcgggatta tattgctttt tgtgctcgcg ggaatattcc
     5341 ccttgtatca atacaactcc catcgcatta atgagaataa aaaattaaaa tatcaaatcg
     5401 aagagcaaaa ggagctggga cctatttatt taactttgct taagattatg gagaataaag
     5461 atttgcgggt tttacccaat cccaagaaag caacaattcc ccgtgtagaa gcgggcaagt
     5521 ttcaggatgc tttccgggca atagcgggta aatcggggtt aatgacaata tcgcttacgc
     5581 cggatttgac tacattggcg gaatcttcca ctttcctttt gcacaatgcc gtgctgaagg
     5641 gtgaatttga caattttcga aaaatgttga tcggattggg ggctgttcca tatctggata
     5701 ggattgagga aattcgtatc cagcagcatc ccgattccat ggaatttaga atgaaaattt
     5761 ggatagccct gggcagttaa tatacataag gtatttatga ctaagttaaa caaaagacaa
     5821 attatcattt tagtaattgc agctttgttt gttttatatg cggtttatga atacttaatt
     5881 gccggcccag cgagtaaaaa agttaaaacc agcgccgatc ctgcggaaat taacactttt
     5941 gtgagcgagc tgaccagtaa tttaataaaa gacaatcttg ctgcagttga cgcatacata
     6001 gtcgggcggg cggaagccga ttggcaaaaa aatcctttct gggaaagaga atcatataag
     6061 aactgggcgg ccagggaagg ggccgccggc ggaagcggtg cagcggctaa aataatttat
     6121 tccggttatg ttgattccgg taataagaaa atggccgtca ttaacggttt ggaatatagg
     6181 ataggggaac aattggaaat ggaaggctat gtattaaaaa atattacgtc ttcgaaggtt
     6241 ttgattgtca ataaaaatac gggaagcgaa atggaagttt ccatacaaga ataaaattta
     6301 gcattggagg ttgtcgactt gaaatgctct aaaagtaata aaga
//
LOCUS       DASVIM010000082         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_541058, whole genome shotgun sequence.
ACCESSION   DASVIM010000082 DASVIM010000000
VERSION     DASVIM010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541058"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="VF305_01680"
     CDS             complement(<1..137)
                     /locus_tag="VF305_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX7415876.1"
                     /translation="MKSIKVKGMTCQHCVMAVTKALGALEGIKNVQVDLKSGVATYEE
                     V"
     gene            complement(281..802)
                     /gene="sodC"
                     /locus_tag="VF305_01685"
     CDS             complement(281..802)
                     /gene="sodC"
                     /locus_tag="VF305_01685"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HEX7415877.1"
                     /translation="MRRLIFIVLVVMVSVGTAMANEKEIQVNLVNDQGLGENIGTISV
                     QDSKYGLILTPKLSNLPQGIHGFHIHQNPDCSNMMTGGKPMAASAAGGHFDPLMTGKH
                     EGPYGNGHSGDLPFLYVDKDGKALLPVLAPRLKVSDLKGRSLMIHAGGDNYSDVPEKL
                     GGGGSRLACGAVK"
     gene            1095..1934
                     /locus_tag="VF305_01690"
     CDS             1095..1934
                     /locus_tag="VF305_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7415878.1"
                     /translation="MIIHCWGARGSIPVSGRQYLHYGGNTTCLEIRTKDDEILIVDAG
                     SGIREAGNFLLAAGRHNFTLLLTHAHWDHVMGFPFFRPIYSRKTNLTVWGCPFAQVSL
                     KEMLSRIMAPPNFPVNFDTIRANISYQDMCIDSYKFGSMIITPIALSHPNQGTGYKFE
                     EDGKCFVFLTDNELGFQHEGGLDYQTYLNFSRGADLLFHDAEFNEEEYKQTRGWGHSV
                     YKDALHLALDAGVKKLGLFHHNQERFDDEIDAIVGDCRKIISQSKKDLECFAVSQSME
                     LVL"
     gene            2002..2394
                     /locus_tag="VF305_01695"
     CDS             2002..2394
                     /locus_tag="VF305_01695"
                     /inference="COORDINATES: protein motif:HMM:NF015166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415879.1"
                     /translation="MVIFFHMLLMPLGTLCFITGVGAAVFFRKKRNWLKVHKYFNSSG
                     FMALCAGGIMAFTYVSEKGGKHLNGIHQIVGFIVLVLTLVTLILGFYQFRAENKIAVR
                     TTHHWSGRLSLLLVIINLTLGLILAKII"
     gene            2431..3060
                     /gene="msrA"
                     /locus_tag="VF305_01700"
     CDS             2431..3060
                     /gene="msrA"
                     /locus_tag="VF305_01700"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7415880.1"
                     /translation="MYTYLVSILIFLVMTNPATGAQKDMKTKTLADVCEEITLGGGCF
                     WCLDAIYREIIGVEKVLSGYSGGSVANPTYEEVCTGSTGHAEVVQITFNPQIITAEKL
                     LRIFFATHDPTTLNRQGADVGTQYRSIIFYRNQKQKDVIDKVIGELKKDKVYKNIIVT
                     EIVPFQSFYAAEDYHQDYYSQNREQGYCRVIINPKLEKFKKTFHDNLKK"
     gene            3102..>3344
                     /locus_tag="VF305_01705"
     CDS             3102..>3344
                     /locus_tag="VF305_01705"
                     /inference="COORDINATES: protein motif:HMM:NF020086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415881.1"
                     /translation="MFLPTTASELKKLKWEKLDIILVTGDAYIDSPYIGVAVIGRILS
                     HAGYRVGIIAQPDVKSDVDIRRLGEPALFWGITGGCM"
BASE COUNT          933 a          698 c          682 g         1031 t
ORIGIN      
        1 tttacttcct cataagtcgc cacgccgctt tttaaatcga cctgaacatt ttttatgcct
       61 tccagagcgc cgagcgcttt ggttaccgcc ataacacagt gctgacaggt cattcccttt
      121 actttaatac ttttcatctt atttctctcc ttccgaagga tttttagata gttttaataa
      181 ttttcaggca aatagatttc tatcttcatc tttaatcgtc cactacctga cagcaatgaa
      241 tgtaagaata gataagaatc gtttttatgg tattcactat ttacttgacg gcgccgcagg
      301 ccagtcggct tcctcctccg ccgagcttct ccggcacatc ggaataatta tctccgcctg
      361 catgaatcat caatgatctt ccttttaaat cggatacttt cagacgaggc gctaatacgg
      421 gcaataatgc tttcccatct ttatccacat acagaaaagg caggtctccc gaatgtccat
      481 ttccgtatgg accttcatgt ttaccggtca ttaaaggatc aaaatgtccg cctgccgcgg
      541 aggccgccat cggcttgccc cctgtcatca tgttgctaca atccgggttt tgatggatat
      601 gaaaaccatg gataccttgc ggaagattgc tcaactttgg ggtaagaatc aaaccatact
      661 tgctgtcctg caccgaaatc gtgccgatat tttctcccaa accctgatca ttgaccaaat
      721 taacctgaat ttctttctca ttggccatgg cagtgccgac agaaaccata actacgagca
      781 caataaatat aagtcttctc atttttttgt ccctctttcg tttgatgatg gatgtttccg
      841 ataaaatctt ttccagtata ttactgcaat atctcccagt acacaatggt aattaatagt
      901 acctttattg atggcagata tctatacact accaaataac cggataatct gcccgcctcc
      961 gaagcccgga cggccgcctt tgaaccggag cattcattcg ataaaaatat tttccttact
     1021 ttttcccgcc agcataggca ttacatccta gttgataaaa aatttattct acattaaggt
     1081 aaaaaagggg ataaatgatt attcactgct ggggtgccag aggttccata cccgtttcgg
     1141 gaaggcaata cttgcactat ggcgggaaca ccacttgtct ggaaataagg acaaaagacg
     1201 atgaaattct tatcgttgat gccggttccg gcattcgcga ggcaggaaac tttctcttgg
     1261 ctgctggccg gcataatttc actcttttat taactcatgc ccattgggat cacgtcatgg
     1321 gttttccttt cttcagacca atttattcac gcaaaacgaa tttgaccgtt tggggttgcc
     1381 cgtttgcgca agtttcctta aaagaaatgt tatctcgcat tatggcgcca cctaatttcc
     1441 ctgtcaattt cgatactatc cgcgcaaaca tatcctatca ggacatgtgc attgattctt
     1501 acaagtttgg ttcgatgatc ataacgccta ttgcgttgag tcatcccaat cagggtacgg
     1561 gttataaatt tgaagaagac ggtaagtgtt ttgtgtttct caccgacaac gagctgggct
     1621 ttcagcatga aggtgggctt gattatcaaa cctatcttaa tttttcacgc ggggccgact
     1681 tgctttttca cgatgccgaa tttaatgaag aagaatataa gcaaactcgc ggatggggac
     1741 attctgtata taaagatgct ctgcatctgg cacttgatgc cggcgtgaaa aaattagggt
     1801 tgtttcacca taatcaggaa agattcgatg acgaaattga tgctatagtc ggtgattgcc
     1861 gcaagataat tagccaaagt aaaaaagatc tcgaatgctt tgccgtttcg cagtcgatgg
     1921 aattggtgct ttagttttaa ctttattgcg tctgccaagt tatccgcgct gcaaatgcgc
     1981 ttattaggga gtatctaaaa aatggttatt ttctttcata tgttattaat gcctttggga
     2041 acgctttgtt ttattacagg tgttggtgcg gccgtttttt ttcgaaaaaa aagaaattgg
     2101 ctgaaagtcc ataaatattt caattcatcc ggttttatgg cgctatgcgc gggcgggatt
     2161 atggccttca cttatgtttc ggaaaagggc ggcaaacacc ttaacggaat tcaccaaatt
     2221 gtcggcttta ttgtgcttgt tctaactctt gttactttga tcttgggatt ctatcagttt
     2281 agggccgaaa ataaaatagc agtaagaaca acacatcact ggtcgggcag gttatctttg
     2341 cttttggtta taattaatct gacgttgggg ctaatcctcg ctaaaatcat ttaaaatatt
     2401 aattatgata ttggaatgct aaaaaaaatt gtgtacactt acttagtctc tattttgatc
     2461 tttcttgtta tgaccaatcc ggcaactgga gcgcagaagg atatgaaaac aaaaactttg
     2521 gcagatgttt gtgaggaaat cacgctgggc gggggctgct tttggtgttt ggatgctatt
     2581 tatagagaaa tcataggggt agaaaaggtt ctttccggct actccggagg ttcagttgcc
     2641 aatccgactt atgaagaagt gtgcacagga tctacagggc acgcagaagt tgtgcaaata
     2701 acctttaatc ctcagattat aactgccgaa aaattgctcc ggatattttt cgcgacccat
     2761 gatccgacga ccttaaaccg tcagggagcc gatgtcggca ctcaataccg ttcgattatt
     2821 ttctaccgta atcaaaaaca aaaagatgtt atcgataaag tcatcggcga gcttaaaaaa
     2881 gataaagtct acaaaaatat aattgtaacg gaaatagttc cctttcaatc tttttatgcc
     2941 gctgaagact atcatcagga ttattacagt caaaatagag agcagggtta ttgtcgggtt
     3001 atcatcaacc ctaaattgga aaaatttaaa aaaaccttcc acgataattt aaaaaaataa
     3061 tagttcttaa tagagtatga tttattttac ggtcaaaaaa tatgttcctg cctactactg
     3121 cttcagagtt aaaaaaatta aaatgggaaa aactcgacat aattcttgtc actggcgatg
     3181 cctatatcga tagtccctat ataggagttg ctgttatcgg ccggattctg tcgcatgccg
     3241 ggtatcgcgt aggaattatc gcccagcctg atgttaaaag cgatgtagat attcgccgtc
     3301 tgggagagcc ggcattattc tggggaatca ccggcggctg tatg
//
LOCUS       DASVIM010000083         2607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_371945, whole genome shotgun sequence.
ACCESSION   DASVIM010000083 DASVIM010000000
VERSION     DASVIM010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371945"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..873
                     /locus_tag="VF305_01710"
     CDS             289..873
                     /locus_tag="VF305_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEX7415882.1"
                     /translation="MEDHIIKIFKESSRVKEAFINENLSKLANVIEALTAALKAGNKI
                     LLFGNGGSAADAQHIAAEFVNRFVIERPPLPAIALTTDTSVITSIGNDYDFSEIFSKQ
                     IRAIGQPGDVAWGISTSGNSANVLKGLEMAKKMGLITVAFTGKDGGDVAKIADFPINV
                     SSSSTARIQETHITAGHAICELVDMKLFQKPDLK"
     gene            894..1487
                     /gene="grpE"
                     /locus_tag="VF305_01715"
     CDS             894..1487
                     /gene="grpE"
                     /locus_tag="VF305_01715"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX7415883.1"
                     /translation="MVKIKKEHTAKHGHPGEFNTENIVADDHTQENADLKKILEEKEK
                     EVTANYDKYLRAVAELDNYKKRAAKEKVDTIKYGNEEIIRDILPFVDSLDRAMEHTGG
                     SDVDAFKDGLKLIQEQLLCCLKKHGVEKIESIGLDFDPSFHEAMMQSDSEKHEENKII
                     NELERGYLLNGRLLRPSKVCVCKKINKENDVCKQIAD"
     gene            1563..2607
                     /locus_tag="VF305_01720"
                     /pseudo
     CDS             1563..2607
                     /locus_tag="VF305_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
BASE COUNT          849 a          471 c          622 g          665 t
ORIGIN      
        1 gtaagtaggg cgcatttacc aaacgcgccg tcgttttgcg atatctccaa aattcaccga
       61 actaaagact ttgagctttc ccattgagcc ctgtttttgc caataattaa gtatactgtc
      121 cccggaattc gcccccaatg atgtcaatat tagtatgctt gtgattgaaa tatctattta
      181 ataaagataa taaaattcaa cctcctgccc ttgacaggag aaacacccgt aaatatatta
      241 gtgccgcttt accagagata attttaaaaa gactccggat gggtaagtat ggaagatcac
      301 ataataaaaa tatttaagga aagcagccgc gtcaaagagg cttttattaa tgaaaaccta
      361 agcaagctgg ctaatgttat tgaggcgctg actgccgcgc taaaagccgg taataagatt
      421 ttgctttttg gcaatggtgg ttcggctgca gacgcgcaac atattgctgc agaatttgtg
      481 aatcgcttcg taatcgaacg tccgcctctt ccggctatag ccctgaccac cgatacttcc
      541 gttattacta gcattggaaa cgattacgac ttttccgaaa tattcagcaa gcaaatcagg
      601 gcaataggcc aacctgggga tgtagcttgg ggtattagta ccagcggtaa ttccgccaat
      661 gttttgaaag gtctcgaaat ggccaagaag atgggattaa taactgttgc ttttaccggt
      721 aaagacgggg gggatgttgc gaaaattgct gattttccca ttaatgtttc ctcttccagt
      781 acggcgcgta ttcaggaaac gcatattacg gccggtcatg ctatttgtga attggtggat
      841 atgaaacttt tccaaaagcc tgatttaaaa taataggata tttttcagag gtaatggtga
      901 aaataaaaaa agaacatact gctaaacatg gtcaccccgg tgaatttaat acggaaaata
      961 ttgttgcgga tgaccatact caagaaaatg cggatttaaa gaaaatatta gaagaaaagg
     1021 aaaaagaagt aacggccaat tacgataagt atctgcgtgc agttgccgaa ttggataatt
     1081 acaaaaagcg ggcggctaag gaaaaggttg atacaatcaa atatggtaat gaagaaatta
     1141 tcagagatat tttacccttt gtggacagct tagatagggc aatggaacat actggcggct
     1201 ctgacgttga tgcttttaag gacgggttga aattgattca agaacagctt ttatgctgtc
     1261 ttaaaaagca cggcgtagaa aaaattgaaa gcatcggcct tgattttgac cccagcttcc
     1321 acgaggcgat gatgcagtct gatagcgaaa agcacgaaga aaacaaaata attaacgagc
     1381 tggaaagagg ttatttactc aatggcagac ttttgagacc gtcaaaagtc tgtgtctgta
     1441 aaaagataaa taaagaaaat gatgtatgta aacaaattgc ggattaatga agaataagga
     1501 ggaaaaaaat gggaaagatt attggaattg atttaggaac aaccaattct tgtgtagcca
     1561 taatggaagg gggagaccct gtcgttatcg ccaatcagga aggcaatcgc accacgccgt
     1621 ctattgtggc catcacggaa aacggtgagc ggctggtggg gcagatagcg aaaagacagg
     1681 ccataacaaa ttcggaaaat accgtttatg ccgtaaaaag gcttatcggt agaaaatata
     1741 gttctaaaga agttcagtac gatataaaga ttattcctta caagataacg gaagcttcca
     1801 atggcgatgc gcaggtaact gtgcggggca aaaattattc gccggccgaa atatcggcaa
     1861 tgattttaac gaagatgaaa cagactgcag aagattatct gggtgaaaaa gtaacagacg
     1921 ctgttattac tactccggca tattttaatg actcacagcg tcaggcgaca aaggatgccg
     1981 gccgtgttgc cggattaaat gttttgcgta tcattaatga acctacggca gcagctctgg
     2041 cttatggttt ggataaaaag aaagacgaaa agattgccgt ctttgacttg ggcggcggca
     2101 cttttgatat atctattttg gaattaggtg aaggcgtctt ttaggtcaag tctaccaatg
     2161 gcgatacgca tttaggcggg gaagatttcg atcagcgcgt aatggattat ctggtagcag
     2221 aatttaagaa agatcagggc attgatttgc gcagcgataa gatggcactg cagcgtctga
     2281 aagaagctgc ggaaaaggcg aaaatggagc tttccacggc gatggaaacc gacatcaatc
     2341 tgccgtttgt cactgccgat gcttccggtc ccaaacatat gaatattaag ctatccagag
     2401 ctaaattaga atctcttgtg gaagagttaa tcaacaaagt tgtcggaccc tgccagaccg
     2461 cgattaaaga tgccaattta accaccaaag atatcgacga agttattttg gttggtggaa
     2521 tgaccagaat gccgcgggtc caacaaaagg taaaagaaat tttcggcaag gaaccgcaca
     2581 aaggcgtgaa tccggacgaa gttgtgg
//
LOCUS       DASVIM010000084         3393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1138976, whole genome shotgun sequence.
ACCESSION   DASVIM010000084 DASVIM010000000
VERSION     DASVIM010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138976"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /gene="dctP"
                     /locus_tag="VF305_01725"
     CDS             <1..645
                     /gene="dctP"
                     /locus_tag="VF305_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX7415884.1"
                     /translation="IASGIRSRAPIRSLADLKGKKIRMAGKALGYVLEKAGAAQALTA
                     PGEIAQQLATGAIDAAAFNTPNIDMSIGLAEVTKYNIGPGWNQPSSVGGVMINKDAWN
                     TLPPDLKRIIEIAAYENLLAMATLSEWDNAMALKKWQEKGIQVTKFSARDLAQIEEWI
                     WEFIAAEALKNPDYDKIATSMFQYMKDFSQARDYEAPYAQGRNPVKWPKLPNLK"
     gene            726..1259
                     /locus_tag="VF305_01730"
     CDS             726..1259
                     /locus_tag="VF305_01730"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7415885.1"
                     /translation="MGGDFFVSLSNFFDNINEWSGRIFCWLVVPLTLIIIIEFITRTL
                     NVPTSWTDESSRFVFGAHFMLVAGYGLLYGSHVRIDFFTNRFSQKTQAFISIICYLLL
                     FFPFMFVWFYYGWSYFYSSWSTLEKSVSFWGPILYPIKFVIPLAAFFLILQGLSEVIK
                     SIVTIRILSILAKEVPE"
     gene            1256..2581
                     /locus_tag="VF305_01735"
     CDS             1256..2581
                     /locus_tag="VF305_01735"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7415886.1"
                     /translation="MSIELIALLMLLAVIIFFFLGHPLAFVLGGVGTIFGFFVMGTTF
                     FSTFMDRIFVGIMGNFTLVAITLFIFMGNLLTISGVADRLFESLRYFLGGINGGLGIA
                     VLAVCIVLSACIGIVGASVVTVGLLTGPMLLRYGYQKELTMGMIAAGGTLGLIIPPSI
                     MLLVMGEQAGLPPGSLFAAAIVPGVSLAIMYMLYVRIRCGLNPELGPPIPAEEIMGMS
                     FSGKLADFVIYALLPLALIIGVALAIFFGLAPPTQVAGIGAFLAFILTVIYRRSTLET
                     FLASLYQTARASTMVLIIMVGASCFTGIFLGMGGGTTIVETLKTAGLSKWGVYFVMMI
                     SLVILGCFIDWVGLIFLVFPIFLPIARLMDFNMLWFVIMMAMYLQTSFLAPPFGYALF
                     YLAGMKFEGVTWEHIYTGIFPFLVLQLIGLLIFTSWPALTLWLPSILYG"
     gene            2945..>3393
                     /locus_tag="VF305_01740"
     CDS             2945..>3393
                     /locus_tag="VF305_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX7415887.1"
                     /translation="MDKKKRIVISGLGTLNSVANNIPDFVHALREGVCGIGAIDLFDT
                     TGFRSKNGGQIKNYIARDYIPRDFSIKRMSRADMLSFAATLEALRDAGLYPLQEELKK
                     EMGIVIGGGSGGLLGAESFYSELLKKGVEKARFSKLAAVYCASSADR"
BASE COUNT          859 a          649 c          768 g         1117 t
ORIGIN      
        1 atcgcgtcgg gtatccgttc ccgtgcgccg atcagaagcc ttgccgatct taaaggtaaa
       61 aaaataagga tggccggcaa agcgctgggc tacgtattgg agaaagctgg cgctgctcaa
      121 gctctgacgg cgcccggcga aattgctcag caattggcca ccggagctat tgatgccgcc
      181 gctttcaaca cccctaatat tgatatgagc attggtctgg ctgaagttac taaatacaac
      241 attggtcccg gatggaatca gccctccagc gttggcggag tgatgattaa taaagatgcc
      301 tggaacactc ttccccctga tttgaaaaga attattgaaa ttgccgcata tgagaatctt
      361 ctcgctatgg ctaccctgtc tgaatgggat aatgctatgg ctcttaaaaa gtggcaagaa
      421 aaaggtatcc aagttacaaa attttccgcg agggacttag ctcagataga agagtggata
      481 tgggaattta ttgctgctga agctctgaaa aatcccgatt acgataaaat cgccacctcc
      541 atgttccaat acatgaaaga ttttagccag gcacgagatt acgaagcgcc ttacgcccag
      601 ggacgaaatc cggtgaaatg gcccaaattg cccaatttga agtaaggttt ttaagtaagg
      661 tttgccgcag attaaaaatc tgcggcaaac cttataaata atccacaaga aaaggggagc
      721 ataaaatggg aggggatttc tttgttagtt tatccaattt ttttgacaat atcaatgaat
      781 ggtcgggcag gattttttgt tggcttgtcg taccattgac cttgatcatt atcattgaat
      841 ttattacaag aaccttgaac gtacctacaa gctggacgga tgaatcaagt aggtttgtct
      901 tcggcgctca ttttatgttg gttgccggtt atggtttact ttacggttct catgttcgca
      961 ttgacttctt caccaatcgt ttctctcaaa agacccaagc atttatttca attatttgtt
     1021 atcttttatt gtttttccct tttatgtttg tctggtttta ttatggctgg agctattttt
     1081 attcatcatg gtcaacttta gaaaaatcgg tgagtttttg ggggccaatt ttatatccta
     1141 ttaaattcgt aattccgtta gcggcatttt ttttaatatt gcagggttta tcggaagtaa
     1201 taaaatccat cgtaacaatt cgtatattat ctatattagc taaggaggtg cccgaatgag
     1261 catagagtta attgcgttat taatgcttct ggcggtaatt atctttttct tcctcggaca
     1321 tccgttagct ttcgtcctgg gaggagtagg aactatattc ggcttttttg taatggggac
     1381 aacttttttt agtactttca tggaccgaat tttcgtaggc ataatgggca attttacttt
     1441 ggttgccatt acgttgttta tttttatggg caatttgcta actatttccg gcgtagccga
     1501 tagactcttt gaatctttgc gttattttct aggaggaatc aacggcggtt taggaattgc
     1561 ggttttggcc gtatgcatcg tcctttctgc ctgtattgga atcgtcggcg catccgtagt
     1621 tacagtgggc cttcttaccg gaccgatgct tttacgttat ggttatcaaa aagagttgac
     1681 tatgggcatg attgctgccg gagggacgct gggtcttata atcccgccca gtatcatgct
     1741 ccttgttatg ggtgagcaag ccggtttacc tccaggtagt cttttcgcgg cagctattgt
     1801 cccgggtgtg tcgcttgcta ttatgtatat gctttatgtt cgcatacgtt gcggtctcaa
     1861 tcccgaattg gggccgccaa ttcccgcgga agaaattatg gggatgagtt ttagtggaaa
     1921 attggctgac tttgttattt acgccctgtt gcctttagct ctaattatag gcgtggcgct
     1981 ggcaatattt tttggtttag cccctcccac acaagttgca ggcattggtg cctttcttgc
     2041 tttcatttta accgtaattt atcggcgctc tacattggaa acattcctag cttcccttta
     2101 tcagacagca agagcaagca caatggtttt aataatcatg gtgggagcgt cttgttttac
     2161 cggaatattt ttaggaatgg gtggcggaac aaccatagtc gaaacattga aaactgccgg
     2221 tttaagcaag tggggagttt atttcgttat gatgatttca ctagttattt tggggtgctt
     2281 catcgactgg gtcggcctaa tattccttgt tttccctatt ttccttccca ttgcccggct
     2341 gatggacttt aatatgcttt ggtttgtgat tatgatggcc atgtatctgc aaacatcctt
     2401 tctagctccc ccatttggtt atgctctttt ttatttagcg ggcatgaaat ttgagggtgt
     2461 aacttgggag catatttata ccggtatttt tcctttcctt gttttgcagt tgataggttt
     2521 gttgatcttt acatcttggc ctgcacttac tctttggctg ccatcaattt tatacgggta
     2581 aacggagttt taatattcaa atgcatgagg cgaaagatag cctgcatcag gttatctttc
     2641 gccttcattt taatgcttct aaaatattaa aataaattga cgcacttctc tgcggtcttg
     2701 tttcaaattt atcctcataa aatggatttg ttctcagcct atgatagaat gacaacataa
     2761 gcggatttct aatatcaagt accggttgaa cttataatcg gaaaaataat caagaattag
     2821 aaatttgtta aaattaggat gtttcgctcc atgggcgggg taattcactt gactttttcg
     2881 tccgcccaat ttaagtttca cccaccgaaa ataaagtgcc aaagtcaata ttgatggatt
     2941 cgctatggat aaaaagaaac gtattgttat tagcggctta ggaacactaa actctgtagc
     3001 caataatatt ccggactttg tgcatgcgtt aagggaaggc gtgtgtggca ttggggcaat
     3061 tgatctattt gatacaacgg gatttcgcag caaaaacggc ggtcagataa aaaactatat
     3121 tgcccgcgat tatattcctc gagatttttc tattaagagg atgtctcggg cagatatgct
     3181 ttcttttgct gcaactctgg aagctctgcg tgatgccgga ctttatcctc tacaggaaga
     3241 attaaaaaaa gagatgggga tagtcatcgg tggcggttcc ggcgggcttc tgggagcaga
     3301 aagcttttat agtgaattat tgaaaaaagg ggtggaaaag gcacgatttt ctaaactagc
     3361 ggcagtttat tgtgcatcct ctgccgatcg cat
//
LOCUS       DASVIM010000085         4452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1225024, whole genome shotgun sequence.
ACCESSION   DASVIM010000085 DASVIM010000000
VERSION     DASVIM010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225024"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1304)
                     /gene="proB"
                     /locus_tag="VF305_01745"
     CDS             complement(177..1304)
                     /gene="proB"
                     /locus_tag="VF305_01745"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX7415888.1"
                     /translation="MAHTKKILIKVGSAVLAGADGLDLKIIDSLVKEISDLVRQEYSV
                     VLVTSGAIVSGKHRLNITGKLKSIPEKQAAAAIGQGRLMRVYSKSFEKNGLYVAQILL
                     TLSDLTDRQRYLNIRNTLSTLMEWGVTPIINENDSVAVDEIKFGDNDNLAAMIANIAE
                     ADLFINLTSAGGLYDCNPAEFKKAKLIPLVSEFTDEIESAATGETTSVGMGGMKSKVL
                     SAKKVTAIGIPCVIAPGKRKKVLADILAGKEIGTLFLPMADRLNSKKYWIAFTLHPRG
                     KLVIDDGAKKALLEKGKSLLPSGIVDVEGDFDLGDPVSCVDREGTPLAKGLVNYNSAE
                     IRKIKGLKTSQISQVLGHKDYDEIIHRDNLAITKQNYRSKL"
     gene            complement(1336..2343)
                     /gene="obgE"
                     /locus_tag="VF305_01750"
     CDS             complement(1336..2343)
                     /gene="obgE"
                     /locus_tag="VF305_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX7415889.1"
                     /translation="MKFVDEAKIYIKAGHGGRGCVSFRREKFVPRGGPDGGDGGKGGD
                     VIFRAAKSHHTLLDLKYQQHQIAKNGGHGSGNHRTGRSAEDLEVIVPVGTIIKNFETG
                     EVLADLSELGQTFIVARGGIGGKGNAHFTTSTHQTPRFAQEGMEGEEFTLKLELKLLA
                     DVGILGFPNAGKSTFISRVSAAKPKIADYPFTTLTPHLGVVKYSNSKSFVLADIPGLI
                     SGAHDGSGMGIQFLKHVERTSLLFHIIDISMEPNANAWSNFTTINKELGHYNPELLEK
                     PQIVALNKIDLPYVRENAKKEVALFKKKGIILHLFSAVTGEGTQEILKKIITILNQAE
                     D"
     gene            complement(2575..2829)
                     /gene="rpmA"
                     /locus_tag="VF305_01755"
     CDS             complement(2575..2829)
                     /gene="rpmA"
                     /locus_tag="VF305_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX7415890.1"
                     /translation="MAHKKGQGSSRNGRDSNSQRRGVKVFGGEKINAGSIIIRQVGTK
                     IHPGTNVGMGKDFTLFSLINGVVKFERLDKTRKKVSVYAS"
     gene            complement(2843..3154)
                     /gene="rplU"
                     /locus_tag="VF305_01760"
     CDS             complement(2843..3154)
                     /gene="rplU"
                     /locus_tag="VF305_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX7415891.1"
                     /translation="MYAVIKTGSKQHKVTEGDVLSVEKLTGDKGTEVVFNEVLMISDD
                     KKVKIGKPFVDGAKVIGEIIDQKKGPKIHVYHMIRRKGFHKKTGHRQELTSMKITKIS
                     M"
     gene            complement(3296..>4452)
                     /locus_tag="VF305_01765"
     CDS             complement(3296..>4452)
                     /locus_tag="VF305_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HEX7415892.1"
                     /translation="SLEETYAKQEFIRQRIKNRKLIVKWHDARMSLLEGMFSRGDERL
                     GELLEKAFRKGCRFDGWTELLRFDLWQEALNESGIKPEDFTRKHNTNEILPWNNIDCG
                     VSREFLLQEKQRAEDTVATADCRFSSCQDCGVCDFSTTKNIFSTAEETQSALPSLPIS
                     NIAAGNENKYRFTFTKLGRARFLSHLELSAALIRALRRSSIALAYSAGYHPHPKISFA
                     TATSVGMESKQEYMDIIAQEYLADLNFLKNEINSALPQVIEVIEIRMLSPSEKTLAQA
                     LQGFEYELYLPADIDSDRLSTIKENVDKFLAAPSFSIQRLSNDKTVTKNIRPFIEKIA
                     LDTSGKKVEFTVRQTQEGSARPTDIITHVLGFNTEEGLQIRVVKTRTLLT"
BASE COUNT         1142 a          962 c          937 g         1411 t
ORIGIN      
        1 taagggatat tttatgaaag atggatggct catttacatt gcgcggttcg ccgacaagtt
       61 tgtgccagaa tctgtgcgtt aagctattct tatgttgtga atcaatagtc tccatgatga
      121 atcactcttt ctataagact atgcataaat gattacttta ttatcatttt ttaagcttaa
      181 agtttgcttc tgtaattttg tttagttata gccaaattgt cgcgatggat tatctcatcg
      241 taatctttgt gtcccagaac ctggctgatt tgggaagttt ttaaaccctt gattttgcgg
      301 atttcagcgg aattataatt gacgagacct ttggccagag gtgttccttc gcggtcaaca
      361 cagcttaccg gatcgcccaa atcaaaatct ccttccacat caacaattcc cgaaggaagg
      421 agacttttgc ctttttccag taatgctttc ttggcgccat cgtcaatgac taacttgccg
      481 cgagggtgca gggtaaaagc gatccaatat tttttactgt ttaaacgatc agccatcggc
      541 aggaatagtg tgccgatttc ttttcccgcc agaatatcgg ccaaaacctt ttttctttta
      601 ccgggagcaa tgacgcaggg aatgccgatg gcggtaactt ttttggcgga caaaactttg
      661 cttttcatgc cgcccattcc cactgatgta gtttctccgg tagcggcgga ttcaatttcg
      721 tcggtgaatt cactgaccag tggaataagc ttggcttttt tgaattccgc cggattgcaa
      781 tcataaagtc cgccggcact cgtcaaattt ataaaaagat cggcttcagc tatgttggcg
      841 atcatcgcgg caagattgtc gttatcgccg aattttattt cgtccacggc gacagagtcg
      901 ttttcattga taataggggt aacgccccac tccatcaaag tggaaagagt attgcggata
      961 tttaaatacc tctggcgatc ggtgagatcg gaaagtgtca gcagaatctg cgcaacataa
     1021 aggccgttct tttcaaaaga tttggaatag acgcgcatca atcgtccctg accaatagcg
     1081 gccgcagctt gtttttcagg aatactttta agcttgccgg ttatattcaa acgatgtttt
     1141 cctgaaacta tcgcgccgga agtcaccaac accacggaat attcctgccg caccagatca
     1201 gatatctctt taacaaggga atcaataatt ttgagatcga ggccgtcagc gccggctaaa
     1261 actgccgagc caactttgat aagaattttt tttgtgtgtg ccaatgtctc ttggcgaata
     1321 ttcttcattg ttcttttagt cttcggcttg atttaatata gtgattattt tttttaatat
     1381 ttcctgtgtt ccttctccgg taacagcgga aaaaagatga agtattatcc cctttttttt
     1441 gaatagcgca acttcttttt tggcgttttc tctcacataa ggaaggtcaa tcttattgag
     1501 agcaactatt tgtggctttt ccagcagttc aggattgtaa tgccccagtt ccttattgat
     1561 tgtagtaaag ttagaccagg cattagcatt gggctccatg gaaatatcaa taatgtgaaa
     1621 caggagtgat gttctttcca catgttttaa aaattgaatg cccatgccgg aaccatcatg
     1681 cgcgccggag ataaggccgg gtatatcggc aagaacaaaa cttttgctgt ttgaatattt
     1741 aacaacgccc aagtgtggtg tcagggtggt gaaaggataa tcggcaattt tgggtttggc
     1801 agcggatacg cgggagataa aggttgattt accggcgtta ggaaagccaa ggatgcctac
     1861 atcagccagc aatttcagtt caagtttaag cgtgaattct tcaccttcca tgccctcctg
     1921 ggcaaaacga ggtgtttgat gggtggaggt ggtgaagtga gcattgccct tgccgccgat
     1981 accaccgcgc gcaacaataa aagtttgtcc taactcggac aaatctgcca gcacttcccc
     2041 ggtttcgaaa tttttgataa ttgttcccac cggtacgata acttccagat cttctgcgct
     2101 tctgccggtg cgatggttgc ccgagccgtg gccgccgttt ttggcaattt gatgttgttg
     2161 atattttaaa tccagcagcg tgtgatgact tttcgctgcg cgaaagataa catcaccgcc
     2221 tttgccgcca tcgcccccgt ccgggccgcc gcgtggcaca aatttttctc tccggaaact
     2281 cacacaaccg cggcctccat gcccggcttt gatataaatt tttgcctcat caacaaattt
     2341 cataaatatt gcccgggtag attttctttt gaaataacat taactgtctt cgaaatgtcc
     2401 gtaccacaat cccgcatgcg cgggataatt cggcttgcca atttcaacgt gtgacctcgt
     2461 gtgaccttta ggttattagg ttatacactt cgtttttgaa attggagtct cattaaaata
     2521 aaaaggcgct tggtgtaagc gcctttaaaa tttaaaaata tttagccatc atttttatga
     2581 tgcgtaaaca cttacttttt tcctcgtttt atcgagtctt tcgaatttta ccacgccgtt
     2641 aatcagggaa aaaagagtga aatctttacc catgcccacg tttgtacccg ggtgaatttt
     2701 tgttcccacc tgccgtataa tgatcgatcc ggcgttaatt ttctcaccgc caaagacttt
     2761 tacgccgcgc ctctgagaat tactatctct gccgttgcgg gagcttcctt ggcctttttt
     2821 atgtgccata atttcctccg acttacatcg aaatctttgt tattttcata ctggttagtt
     2881 cttggcgatg tccggtcttc ttatgaaagc ccttacggcg tatcatgtgg taaacgtgaa
     2941 ttttcggacc tttcttttga tcgataatct caccgataac cttggcgcca tccacaaaag
     3001 gtttgccaat ctttactttt ttgtcatcgg aaatcatcag gacttcgtta aaaactactt
     3061 ccgtgccctt gtcaccggtt agcttttcta cggatagaac atcaccttcg gttactttgt
     3121 gttgttttga ccccgttttt attactgcgt acatacactt atccttacat tagaattaag
     3181 attggtgctt atagataatt tgtatccgtt tgtcaataaa atttagttgt taaataaata
     3241 ttaattaata ggtgtggata taaggctaag atatcgattt aatgtaaatt tagtgtcagg
     3301 taagcaatgt cctcgttttg acgacacgaa tttgcaatcc ttcttccgta ttaaatccca
     3361 gaacatgcgt aattatatcg gtaggtctgg ccgagccttc ttgcgtttgc cgtactgtga
     3421 actcaacttt tttgccggag gtatcaagag ctattttttc tataaaaggg cggatatttt
     3481 tcgtgacggt tttatcattg gataaccttt gaatactaaa tgatggcgca gccagaaatt
     3541 tatcaacgtt ttctttaatg gtggacaaac gatcagaatc aatatcggca ggaagatata
     3601 gctcatattc aaatccctgc agcgcttgag cgagggtctt ttcactagga gaaagcatcc
     3661 taatttctat aacttcaatg acttggggca gagcggaatt gatttcgttt ttcaaaaaat
     3721 ttaaatcggc taagtactct tgggcgataa tgtccatgta ttcctgcttg ctttccatac
     3781 ccaccgaggt ggctgtggca aaggatattt taggatgagg gtgatagcct gcggaataag
     3841 caagtgcaat actgctgcgt cgcaaagcac ggattaaagc tgcggatagt tccaaatgtg
     3901 ataaaaagcg agctcgtcct agtttggtaa aggtaaaacg atatttgttc tcattgccgg
     3961 cagcgatatt ggaaattggc aacgatggta aagctgattg agtttcctca gccgtggaaa
     4021 aaatattttt tgtcgtggag aaatcgcaaa ctccacagtc ttgacaactg gaaaatctgc
     4081 aatccgctgt ggccactgta tcttccgctc tttgcttttc ctgcagcaaa aattcacggc
     4141 ttacaccgca gtcaatatta ttccacggca atatttcatt tgtattgtgc ttgcgtgtga
     4201 aatcttccgg tttaataccc gattcattta gtgcttcctg ccagagatca aagcgcaaaa
     4261 gctcagtcca accatcgaaa cggcaacctt tacgaaaggc tttttccaaa agctctccca
     4321 atctttcatc gccccgcgaa aacattcctt ccagtaagct cattcgggca tcatgccatt
     4381 taacgatgag tttgcggttt ttgattcgct ggcgaatgaa ttcttgtttg gcataagtct
     4441 cttccaaaga ta
//
LOCUS       DASVIM010000086         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_883574, whole genome shotgun sequence.
ACCESSION   DASVIM010000086 DASVIM010000000
VERSION     DASVIM010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883574"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VF305_01770"
     CDS             complement(<1..744)
                     /locus_tag="VF305_01770"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415893.1"
                     /translation="MNFIDSTTNKAAKFFKQLLENHNFPLIFLKNSIFQRWAIGIILC
                     LILAIILAPEIRFSAPKFKQGMIASHDTKADRDFLVEDQKSTEDKKNDVAENIKPVYD
                     YDSKVAANIKTKLAKALTYAAEDYKSSLKEKPQEVSPVDISKSQKGKRSLEANLGVLL
                     SSEEFYVLKEHKFSPELQQKLFRLIFPFYDRKFITNSIFSKAEKEKGITVRNLNTQNE
                     LEIKDISKILNTQEIDAELLRRTNAIFNDA"
     gene            complement(750..1745)
                     /locus_tag="VF305_01775"
     CDS             complement(750..1745)
                     /locus_tag="VF305_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7415894.1"
                     /translation="MQQTIKNDSIEKISFADHHLLKNLCGEHDKHLKLIENLEPVQIK
                     AWGNNISIVGKNGNVRKISVMLQQLYSIMEKGWHIHSSDIDYAHRILSEDVTFELARI
                     FLDTVFISSKKRTITPKSIAQRLYIEYMRNFDMVFAIGPAGTGKTYLAMAMAVSALLE
                     KKVQRIILARPAVEAGERLGFLPGDLAEKVNPYLRPLYDALYDMMDMERATALINKGL
                     IEVAPLAFMRGRTLNDSFVILDEAQNTTSEQMKMFLTRLGFGSKAVITGDVTQVDLPQ
                     ERVSGLIEAERILKNIARMRFVHFSEIDVVRHPLVQDVIKAYAVLDKERKTNSKK"
     gene            complement(1840..3081)
                     /locus_tag="VF305_01780"
     CDS             complement(1840..3081)
                     /locus_tag="VF305_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HEX7415895.1"
                     /translation="MIDADKFKRISRRIDSYREAMIELQTALSAVPAVGPENGGEGEL
                     LKANLLEKRLQELGFKNFRHYDAQDKSVPSGIRPNFITTIEGKNKTGFVWIITHLDIV
                     PPGEIKLWTSDPYKAYVKDSYIYGRGVEDNQQDMVASIFAAKAILDEGIALAKSIGLA
                     FVADEETSSKMGACYMLDRADQPFKKDDLIVVPDSGNREGSLIEVAEKSMLWLSFKTK
                     GKQCHGSNPQLGNNAFVAASGLVTKLASLKKSFPQSDPLFDPQASTFEPTKKEANVGN
                     INTIPGEDVFYMDSRVLPDYQLPDVMAAIKKIVSRMEKKFKVQIEISPVQYLQSPKPT
                     SPDAPIVRALTEAIGTVYGIKAFAGGVGAGTIAAYFRKMDFPVAVWSKNNQTAHQPDE
                     NCSIDNMIGNAKVFAYLFLRE"
     gene            complement(3103..4548)
                     /locus_tag="VF305_01785"
     CDS             complement(3103..4548)
                     /locus_tag="VF305_01785"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7415896.1"
                     /translation="MTRKIINLSTKLTHVLFILFFLAGGATPAALAAGSRFPNPDKIK
                     YLPLAFNLPQVQRTVLENGIVIYILEDHELPIVNINAVIKTGTIHDPEDKEGVAELTA
                     YVMRTGGTAELNSEEVDSQFDFLAASAAISMSLESANVDFSVLNKNLDRGLDLLSQIL
                     IKPAFEQNKFDLAKQLKDEKLRRLKDEPQSLAFREFNRLIYRGDPRGRFPSHKSLANI
                     DREDLIRFHRRFFQPNNIMFAVTGDITRQQAIDKLRHYFGNWKTESMPLQTALPPQKS
                     NAGLYYINKEIPQSTIISGQFAPSKTDPNFYAFTVLDFIAGSGGFPSRIFNAVRNNEG
                     LAYSAGSFYRARPAYGVFGTYAFTKTSSTLKTLSLINTVLDNIKSNTLTPKELAWAKT
                     SINNGFIFSFTSAEKIADLQMKIEYEKLPADFLSTYRNRIESVTLKDVNRVAEKYLDK
                     TKNIVLLLGDTKQFDEPSTKTGQPVLITTED"
BASE COUNT         1197 a          961 c         1027 g         1460 t
ORIGIN      
        1 ggcatcatta aaaatagcgt ttgttcttct taaaagttcc gcatctattt cctgcgtatt
       61 taatatcttg gaaatatctt tgatttccag ttcattttga gtatttaaat ttcttacagt
      121 aatgcctttt tccttttctg ctttgctaaa aatactatta gttataaatt ttctatcata
      181 gaaaggaaaa attaaacgga aaagcttttg ctgtagttcc ggagaaaact tgtgttcttt
      241 taaaacataa aattcttccg aacttaagag aactcctaaa ttcgcttcca agctcctttt
      301 tcctttttgt gatttactta tatcaacggg gcttacttcc tgtggctttt ccttaagtga
      361 acttttgtaa tcttcagcgg cgtaggtgag agctttggct aactttgttt tgatgttggc
      421 ggcgacttta ctgtcgtaat cataaaccgg tttgatattt tctgcgacgt catttttttt
      481 gtcttcggtt gatttttgat cttcgacaag gaagtcgcgg tcggctttag tatcatgaga
      541 ggcaatcatt ccttgtttaa atttcggtgc cgaaaaacga atttccggag ccaggataat
      601 tgccaggatt aagcagagaa ttatgccgat agcccatctt tggaaaatgc tgtttttcag
      661 aaaaataagg ggaaaattat gattctccaa taactgcttg aaaaattttg ccgctttatt
      721 tgtcgttgag tcgataaagt tcataaaatt tatttctttg agttggtttt tctttcctta
      781 tccaaaacgg cataagcttt gatgacatct tgaactaatg ggtgccggac aacgtcaatt
      841 tccgaaaagt gtacaaagcg catacgggca atatttttta ggatccgttc cgcttcgatt
      901 aatccggaaa ctctttcctg gggcaggtca acctgcgtaa catctccggt aattaccgct
      961 ttcgagccaa atccgagcct tgttaaaaac attttcatct gctcggaagt ggtattttgc
     1021 gcctcatcta aaataacaaa agaatcgtta agagttctgc cgcgcataaa agcgagaggt
     1081 gcgacttcga tcaatccttt attgatcagt gcagttgctc tttccatgtc catcatatca
     1141 taaagtgcat catatagggg gcgcagataa ggattcactt tttcagccag gtctcccggt
     1201 aaaaaaccta atctttcacc tgcctctact gcgggacgtg ccagtataat ccgctggact
     1261 ttcttttcca gtaaggctga tacggccata gccatggcca gatatgtttt acccgttccg
     1321 gcgggaccta tggcaaaaac catatcaaaa tttctcatat attcaatata aagtctctgc
     1381 gcaatacttt tgggggtaat tgttcttttt ttggaagaaa taaaaacggt gtcgagaaat
     1441 atacgcgcca attcgaatgt tacatcttcc gaaagaatac gatgagcata gtcaatatcg
     1501 gaggagtgaa tatgccaccc cttttccatt atcgaataaa gctgttgcag cataacgctg
     1561 atcttacgaa cgttaccgtt tttcccgaca atggaaatat tatttcccca tgcctttatc
     1621 tggaccggtt ccagattctc gataagtttt aaatgcttat catgttcacc gcaaagattt
     1681 ttcagcaaat ggtgatcggc aaaagaaatc ttctctatgg aatcattttt aattgtttgc
     1741 tgcaaatagc tatcaacctc taaagttatg gttagtctta ttttgccctg aaattaagcg
     1801 tgagcttcta tcactttgat atgcccaatg caatacaatt tactcccgca aaaacagata
     1861 ggcaaagact ttggcattgc ctatcatgtt atcgatagag cagttttcat ccggctgatg
     1921 agctgtctgg ttgtttttag accaaacagc aaccggaaaa tccatcttac gaaaataagc
     1981 ggcaatggtt ccggcgccga ctccgccggc aaacgctttt attccgtaaa cagtgccgat
     2041 ggcttcggtg agtgcccgga caatgggagc atcgggagag gttggttttg gcgattgcag
     2101 gtattggacc ggagatattt cgatttgcac cttaaatttc ttttccatgc gtgaaacaat
     2161 cttttttatt gccgccataa cgtctggcag ttgataatcg ggaagcacgc ggctgtccat
     2221 ataaaaaaca tcttcaccgg ggatagtgtt gatattgccg acgttggctt ctttttttgt
     2281 cggttcaaaa gtgctcgcct gcggatcaaa aagcggatcg gactggggaa aggatttttt
     2341 taatgaagcc agttttgtaa ccagaccaga ggccgcgaca aacgcgttat tgcccagttg
     2401 cggattgctt ccgtgacatt gcttcccctt tgttttaaaa gaaagccaga gcatgctttt
     2461 ttcggcgacc tctattaatg agccttcccg gtttccggaa tcgggaacaa caatcaaatc
     2521 atctttttta aacggctgat cggcgcgatc cagcatatag caagcgccca ttttgctgga
     2581 tgtttcctcg tcggcaacga aagccaggcc gatggactta gccaaggcga tgccttcatc
     2641 cagaatcgcc ttggcggcaa aaatggaagc gaccatatcc tgctgattgt cttccacgcc
     2701 gcggccgtaa atatagctat cttttacata agccttgtat ggatcgctcg tccaaagttt
     2761 tatttctccg ggaggcacta tatccagatg agttataatc caaacaaatc cggttttgtt
     2821 tttaccttcg atcgtggtta taaaattcgg acggatgccg gaagggacgc ttttatcttg
     2881 ggcgtcatag tgccggaaat ttttaaagcc cagttcctgc aggcgttttt ccaatagatt
     2941 agccttcaat aattcgcctt caccgccgtt ttccggtccc acggcgggaa cggcgctaag
     3001 agccgtttgc agttcgatca ttgcctcgcg gtaggagtcg attctccgag atattcgttt
     3061 aaatttatct gcatcgatca taaaaatctt ttcatgaaaa aactaatctt cggtagttat
     3121 taaaacaggc tgtccggttt tggttgaggg ttcgtcgaac tgtttggtgt cgcctaaaag
     3181 cagaacgatg ttttttgttt tatccagata tttttcggcg actctgttga catctttaag
     3241 agtgacgctt tctattctat tgcggtaagt gctaagaaaa tccgccggta atttttcata
     3301 ttcgattttc atttgcaggt ccgcaatctt ttcggcgctg gtaaaagaaa aaataaagcc
     3361 gttattgatc gatgttttgg cccaggcgag ttcttttggg gttagagtgt tggatttaat
     3421 attatccagc acggtattaa tcaaggaaag ggttttcagc gttgaggatg ttttcgtaaa
     3481 ggcataagtg ccaaaaactc cgtacgcggg tctggcccgg taaaagctcc cggcgctgta
     3541 ggccaggcct tcattattac gcacggcgtt gaaaattcgt gaggggaaac cgccgctgcc
     3601 ggcaataaaa tcgagaacgg taaaggcgta aaaattagga tcggttttgc ttggggcaaa
     3661 ttggccgctg atgattgtgg attgaggtat ttctttgttt atgtaataaa gcccggcatt
     3721 ggatttttgc ggcggcagag ccgtctggag aggcatgctt tcggtcttcc agtttccaaa
     3781 gtaatgtctt aatttatcta tcgcttgctg cctggttata tctccggtta cggcaaacat
     3841 aatattatta ggctggaaaa accgtctgtg aaatctgatt aaatcttcac ggtcgatatt
     3901 ggcgagcgat ttatgggaag ggaagcggcc gcgcgggtcg ccgcgataga taaggcgatt
     3961 gaattcacgg aaagcgaggc tttgcggctc gtctttcagt cttcttagtt tttcatcctt
     4021 tagctgtttg gctaaatcaa acttattttg ttcaaaagcg ggcttgatga gtatttgaga
     4081 cagcagatca agcccccggt ccaggttttt gtttaaaacg gaaaaatcca cattagccga
     4141 ttccagggac atagaaattg ccgcggaggc ggccagaaaa tcgaattggc tgtcgacctc
     4201 ctcgctgttt aactctgccg ttcctccggt tctcatcaca taggcggtta attcggcaac
     4261 tccttctttg tcttccggat catggattgt tccggtttta atgacggcgt tgatattaac
     4321 aatgggcaat tcgtgatctt ccagaatata aatcacaatc ccgttttcta aaacagtgcg
     4381 ctggacctgc ggcaaattaa atgcaagcgg caggtattta attttgtccg gattggggaa
     4441 gcggctaccg gccgctaagg ctgcgggtgt agcgcctcca gccaagaaga ataaaataaa
     4501 aagcacgtgg gtcaattttg ttgatagatt aatgattttt ctggtcataa aagcccgtgt
     4561 ttaatccttc tttttattta atacggctat tgtgcggttt tctttattca gataaaattt
     4621 tgccgccttt tgaatatcga gagcg
//
LOCUS       DASVIM010000087         2755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_799165, whole genome shotgun sequence.
ACCESSION   DASVIM010000087 DASVIM010000000
VERSION     DASVIM010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799165"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VF305_01790"
     CDS             complement(<1..188)
                     /locus_tag="VF305_01790"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX7415897.1"
                     /translation="MQATHKTQLEQFASFGSILMLSAWGFAMVIASFLFLYVGHLLDT
                     LLGTAPNFMLGLFFLAIFL"
     gene            complement(252..527)
                     /locus_tag="VF305_01795"
     CDS             complement(252..527)
                     /locus_tag="VF305_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415898.1"
                     /translation="MKKTIGTMMVTAAVVLLTATFCFAEFAAGQAGNFPYFQLGCLII
                     GGLIVVSLKRKYEKMYTTEAVGVFALYTILMALFTNPVIEAVKTIVS"
     gene            complement(845..>2755)
                     /locus_tag="VF305_01800"
     CDS             complement(845..>2755)
                     /locus_tag="VF305_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX7415899.1"
                     /translation="SGFKTKQILAAPIYNSNQLMGVIQVLNRKISEENFSEEEIGLAL
                     EIAEVLGIAFYNQQRFAHRKTRFDYLVSHDLIKEEELERAWEESQEQKETMENFLMRK
                     YRISKDDIGRAFEEFYHCRFIQYNDKTVIPVDLIKNLKKDYLRRELWVPLERIDGKIH
                     VIVDNPNNIIKRDTIESLLKNKQVKYDVALAEDIIKFINRFYHVSDEEHSFTEILGRL
                     DDEDTTIEEEEGGEMITESDSVIMQLVNKIINDAYGRRASDIHIEPNVDKKNVEIRFR
                     VDGDCALYQTVPFSYRAAVISRIKIMSNLDITVKRIPQDGKIKFRRSTGEEIELRVAT
                     IPTQGGVEDVVMRILAKGEAMPLEDMALLPRNYNELINIISQPYGMILAVGPTGSGKT
                     TTLHAALHHINRPDRKIWTAEDPVEITQYGLRQVQVQPKIGFDFAAAMRSFLRADPDI
                     IMVGEMRDFETAKTGVEASLTGHLVFSTLHTNSAPETIVRLLDMGIDPLNFADALLGI
                     LAQRLVRTLCKKCKEQYHPTKEEYEEIAESYDREAFDKLNIPYNNDFKLYRPKGCDIC
                     DKTGYRGRMGIHELLVGTDDIKRMVQKHETVEVMRNKARSEGMTTLLQDGILKSLQGF
                     TDFKQVRRVCIK"
BASE COUNT          763 a          606 c          558 g          828 t
ORIGIN      
        1 aggaagatgg ctaagaagaa taaacccagc atgaagttag gtgcggtccc caaaagggta
       61 tccagcaggt gacctacgta aaggaacagg aaagaagcaa tcaccatcgc gaatccccag
      121 gccgagagca tcaagatcga accgaaacta gcgaactgct ccaattgtgt tttgtgtgtt
      181 gcttgcattg tccgtccctc cttccttagg cgagccgcct tcacttagca gctcgattca
      241 tctggttcct cttagctgac gatggtcttt accgcttcga tcaccggatt ggtgaaaagc
      301 gccatcaaga tggtgtacag agcgaagaca ccgactgctt ccgtagtata catcttttca
      361 tatttacgct ttaaagaaac cacaatcaaa ccgccgatga ttaagcagcc cagctggaaa
      421 taggggaaat tgcctgcttg acctgccgca aactcggcaa agcagaaagt tgctgttaaa
      481 aggacaaccg ccgccgttac catcattgtt cctattgtct ttttcatgat ctgtttcctc
      541 ctttaagctt atttgccctt atactaatgg aattagcgtg ccaaaaaact aaagttcgct
      601 actaatagtt ataagtaatg gataataata actatttact taataattac taaattatta
      661 atcagaaaat cccttcctct ccgctctctt tttaaataaa atattaaaag ccgttgaacg
      721 aaatgtggaa agagtattca aaaaatgatt ttcgtaaagt tgattaagaa atagagaaat
      781 ttcaaaagtc aaaataagaa ataaagctat gtgaatggaa acagggaatt aaggaaaaat
      841 aaaattattt aatacaaacg cggcgcacct gtttaaaatc ggtaaaaccc tggagggact
      901 ttaaaatacc gtcttgtaaa agtgtcgtca tgccttcgct cctggctttg ttccgcatga
      961 cttccacggt ttcatgcttt tgcaccattc ttttgatatc atcggtgccg actaaaagtt
     1021 catgaatacc cattctgcct ctatatccgg tcttatcgca tatatcacaa cctttcggcc
     1081 ggtaaagctt gaagtcgtta ttgtaaggaa tatttaattt atcgaatgct tcccggtcat
     1141 agctttcggc aatttcttcg tattcttcct tcgtaggatg atattgctcc ttacacttct
     1201 tgcaaagagt tcgtacaagg cgctgggcga gaatgcccaa caaagcatcg gcaaaattga
     1261 gggggtcgat gcccatatcc agtaggcgta caatagtttc cggggcactg ttggtgtgca
     1321 gagtgctgaa caccaggtga ccggtaaggg atgcctcaac gcctgtcttg gctgtttcaa
     1381 aatcacgcat ttcgccgacc atgataatgt caggatcggc gcgcaaaaag gagcgcatag
     1441 cagcggcaaa atcaaagccg atcttcggtt gcacctgtac ttgacgcaaa ccgtattgag
     1501 taatttctac cggatcttcc gctgtccata tttttctgtc tggccggtta atgtggtgca
     1561 aggccgcatg tagcgtagtt gttttaccgg aaccggtagg accaacagct aaaatcatgc
     1621 cgtaaggttg ggaaataata ttaatcagtt cgttgtaatt gcgcggcaat aaagccatat
     1681 cttccagcgg cattgcttcg cctttagcta aaatacgcat aaccacatct tccaccccgc
     1741 cttgggttgg aattgttgcc acacgtaatt caatttcctc tcccgtggaa cgcctgaatt
     1801 taatcttacc gtcctgagga attcttttaa cggttatatc caagttggac atgatcttga
     1861 tacgcgaaat aaccgctgca cggtaactaa aagggactgt ttgataaagg gcacagtctc
     1921 cgtcaacacg gaaccggatc tccacatttt ttttgtcgac attgggttca atatgtatat
     1981 ccgaagcgcg tcgaccataa gcatcattaa taattttatt gacgagctgc atgatgacgc
     2041 tgtctgattc cgtgatcatt tcgccgcctt cctcttcttc tatcgtcgta tcttcatcat
     2101 ccagtcttcc caatatttcc gtaaaagagt gttcctcatc ggagacatgg taaaagcgat
     2161 tgatgaactt aattatgtct tcagctaaag cgacatcata ttttacctgt ttgtttttta
     2221 aaagactttc gatagtgtct ctctttataa tgttgttcgg gttatccacg attacatgaa
     2281 ttttgccgtc gatcctctcc agtggcaccc aaagttcacg gcgcaagtag tcctttttta
     2341 aatttttaat taaatcgacc gggataacgg ttttgtcgtt atattgaata aaacggcagt
     2401 gataaaattc ctcgaaagca cgaccaatat catctttgga tattctatat ttacgcatga
     2461 ggaagttttc cattgtttct ttttgttcct gtgattcttc ccaagctctt tccaactctt
     2521 cttctttaat tagatcgtgg ctgactaaat aatcaaaccg tgttttccgg tgtgcaaaac
     2581 gttgttggtt ataaaaggca atgcccagaa cttcggcaat ttcgagcgct aatcctattt
     2641 cttcttcgga aaaattttcc tcgctgatct ttctgttgag aacctggatg acacccatga
     2701 gttgattgct attgtaaatg ggagcggcaa gaatttgctt ggttttaaag cctga
//
LOCUS       DASVIM010000088         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1225518, whole genome shotgun sequence.
ACCESSION   DASVIM010000088 DASVIM010000000
VERSION     DASVIM010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225518"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1476)
                     /locus_tag="VF305_01805"
     CDS             complement(175..1476)
                     /locus_tag="VF305_01805"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX7415900.1"
                     /translation="MKYDFSIDNLGERLIESPLNVSNFTQDKKRLLFNSYLDNYSPCN
                     DPDGQPLSLELAGPRNKIFFDPDLTKAAIVTCGGLCPGINDVIRSITMTLFYRYGVKD
                     IAGIKYGLRGLNPIYGHELVKLSPEIVDDITSIGGSILSTSRGPQDAVQLVNSLSGLN
                     INILFCIGGNGTIRAAEKITAEITKRNLKIAVVGIPKTIDNDLNLIQRSFGFDTAIEK
                     TVGAIKSAHVEAKGAFNGIGLVKIMGRLSGHIAAHAALAQNDTNFVLIPEVPFDLEGK
                     KGFLKALETRMKSSGHAVVLVAEGAGQNLFQKSASASERDASGNIRLFDIGHFLKSSI
                     EDYFNKIDLEINLKYIEPSYLIRSVPANASDGIYCSSLGQYAVHAGMAGKTGLLVALM
                     KDEYVHLPLNAAVSRRKIDPQDNVWLRVLETTGQPPSMKNR"
     gene            complement(1480..>2346)
                     /locus_tag="VF305_01810"
     CDS             complement(1480..>2346)
                     /locus_tag="VF305_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415901.1"
                     /translation="FTTNSQDSVASHFQYSAPLHGIYCFLQGKQVFFDSLIKKLESTN
                     GLFLNNCEVLSIKKGGLVEVDVIDKHGIISRISAKNLIVSTKWKGMHLLLERKKKINM
                     DDWVRPIKVSHYPFTIHLGIRQKCIPEKMARHVAVISDVNKDIYDNNLIILELCMPQD
                     EKTISLAKNSLTATVFLSNDRVIWSDENLEANAQSIIGRLEYYLHFLKDNIEIFDIEK
                     SIDISKKQRDIINPKYQLRNSFISGFAAKNNKTRFGNVYLTGASLLTDAGFEGEIISG
                     INAVARIAVKRK"
BASE COUNT          683 a          476 c          449 g          738 t
ORIGIN      
        1 aggcgtataa tgaatatacg ttgagcggtg tggtttgcag cgcaacaaag cagatgagcg
       61 ccctttaggg tatgagcgtt ttgaaaaagc cattcggctt tttccggtag tgaatatatt
      121 taagaatgat tgcgcctccc ttgcgggaat gcgcgcccgg tcaacagccc cgtgtcaccg
      181 atttttcatt gaaggaggtt gtcccgttgt ctccaacacg cgcaaccaaa cattgtcctg
      241 aggatcaatt ttacggcgag aaacagctgc atttaaaggt agatgtacat attcatcttt
      301 cattaaggca accaggaggc ctgttttccc cgccatcccg gcatgcactg catattggcc
      361 gagcgaactg caataaatgc catccgaggc attggccgga accgagcgaa tcagataact
      421 aggctcaata tattttagat taatttccag atcaatttta ttgaaataat cctcgatgga
      481 acttttcaaa aaatgtccaa tatcgaacaa gcgaatgttt cccgaagcat ctctttccga
      541 agctgaggca cttttttgga agagattttg tcccgcgcct tccgcaacta aaactacggc
      601 atgaccgctg gatttcatac gcgtctccag agccttcaga aatccttttt taccttcaag
      661 atcaaaaggc acttcgggaa ttaaaacaaa atttgtatca ttttgcgcca gagccgcatg
      721 ggcggcaata tggcctgaaa gtcttcccat tattttaacc aaaccaatgc cgttgaatgc
      781 tcccttggct tctacatgag cgcttttgat agctccaaca gttttttcta ttgccgtgtc
      841 aaaaccaaat gatctttgga ttaaattaag gtcgttatca attgttttcg ggataccgac
      901 aacggcaatt tttaaattcc gctttgttat ttcggcagtt attttttcgg ctgcccgtat
      961 tgtgccattg ccgccgatac aaaaaaggat attaatattt aatccggaaa gcgaatttac
     1021 tagttgaacg gcatcctgcg gtccacgcga agtggaaaga atgcttccgc cgatgctggt
     1081 tatatcatct actatttccg gggataattt gactaattca tgcccataga ttggatttaa
     1141 tccccgcaaa ccatatttaa tgccggctat atctttaacg ccgtaacggt agaagagtgt
     1201 catcgttatt gatctaatga catcgttaat acccggacaa agaccgccac aagtaacaat
     1261 tgccgctttt gtcaggtctg gatcaaaaaa tattttattc cgtggaccgg caagttccag
     1321 ggaaagcggc tgaccatctg gatcgttgca aggtgaataa ttatcaagat aagagttaaa
     1381 taataatctt tttttatctt gcgtaaaatt gctaacgttt agtggcgatt caattaacct
     1441 ctctccgaga ttatcaatgg agaaatcgta tttcatagat catttccttt ttactgcaat
     1501 tctggctaca gcattgatac cggaaataat ttctccttca aaaccagcgt ctgtcaataa
     1561 tgatgcaccg gtgaggtata cgtttccaaa acgggtttta ttatttttcg cggcaaaacc
     1621 ggaaataaaa gagtttcgta attgatattt tggattaata atatcgcgct gttttttgga
     1681 aatatcgatt gatttttcta tatcgaagat ttcaatatta tcttttaaaa aatgcagata
     1741 atattccagg cggccaatta ttgactgagc atttgcttct aagttttcgt cagaccaaat
     1801 aactcgatca ttagaaagaa aaacagtcgc tgttagtgaa ttttttgcca gggagattgt
     1861 tttttcatct tgaggcatgc ataattccaa aatgatcaga ttattatcgt aaatatcttt
     1921 atttacatcg ctaattaccg ctacatgtct ggccattttt tccggaatac atttttggcg
     1981 aatgccgaga tgaattgtaa aaggataatg agaaacttta attggcctga cccagtcatc
     2041 catattgatt ttctttttac gttcaagtaa taaatgcatc cctttccatt tcgtgctgac
     2101 aattaagttc tttgccgaaa ttctagagat aatcccgtgc ttatcgataa catctacttc
     2161 gactaatccg ccttttttaa ttgacaatac ttcgcaatta ttaagaaata gaccatttgt
     2221 agattccaat ttttttatta aagaatcaaa aaatacctgt ttcccctgta aaaaacagta
     2281 aataccgtgc agaggtgccg aatattgaaa atgtgaggca acagaatctt gactgtttgt
     2341 tgtaaa
//
LOCUS       DASVIM010000089         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_971171, whole genome shotgun sequence.
ACCESSION   DASVIM010000089 DASVIM010000000
VERSION     DASVIM010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971171"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1670
                     /locus_tag="VF305_01815"
     CDS             207..1670
                     /locus_tag="VF305_01815"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7415902.1"
                     /translation="MEEIKKSEESAIEPATVPSEISMEGLTPELRAACDRAGWEILTP
                     VQAKSIPYILAGRDMMVQSRTGSGKTGAYLLPIMQKINMQQNAAQALVLVPTRELALQ
                     VSKEAQMLTQGTNLRTAVVYGGVGYNAQLEAFRGGAQLVVGTPGRILDHLLKNTLSLD
                     HLKILIFDEADRMLSMGFYPDMVKIRKYIPSREILSSMFSATFPPQVIRLAAQFLRQP
                     QLLSLSGNQVHIAEIEHIYYVVPGMRKERSLVRIIEIENPASAIIFCNTKDTVHYLSV
                     VLKRFGYDADELSSDLAQSAREKVMARVKRGALRFLVATDVAARGIDIPDLSHVIQYE
                     PPEDPEAYIHRAGRTGRMGSSGVAISLVAEMEKFKLQSIARYYNINMEERQLASDEEL
                     ERVVAERITALLESRLRARDNLQIERMQRFLPLARNLAQADDELALIAMLLDDYYQQS
                     LHAPLVQPLAVTDMTIGQHQKSKQRRSHPENKKERKK"
     gene            1677..>2196
                     /locus_tag="VF305_01820"
     CDS             1677..>2196
                     /locus_tag="VF305_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7415903.1"
                     /translation="MQFNFFRLLKNKKSIHFICFGIFLFLFVSCASTSKEIIPSKTAK
                     IAVVLGAGVSKGFAHIGVLKVLESHKIPIDMIVGTSVGSFVGSLYAYGFNPYELQTIA
                     FAIQKDDVADYIMPDNGFIKGEKLENFVNNKLKYTPIDKFKIPFYAVATNIQTGEEMV
                     FGRGNAGKAVRAS"
BASE COUNT          620 a          446 c          510 g          620 t
ORIGIN      
        1 cagggcttta aaaagcctct taatgaattg caaaatacac tatattaatc tagagcgact
       61 gtccagagat tttattgttt tcgataaatt tctcttggaa aagaatgttt ttacgtttat
      121 aatcaattct tccttgattc ctgcgcaaca ctaaactaaa tgattttaat taattatttt
      181 gttgaggaag agagattttg ttttttatgg aagaaattaa aaagagtgaa gaaagcgcta
      241 ttgaaccggc cactgttccc tccgaaattt ccatggaagg gctcacgccg gaactgcgtg
      301 ccgcttgtga tcgcgcgggc tgggaaattc tcacgccggt acaagccaaa tcaatccctt
      361 acattcttgc gggtcgtgac atgatggttc aatcacgtac cggcagtggt aaaaccggcg
      421 cttacctgtt gcccattatg caaaaaatta atatgcagca aaatgccgcc caggctttag
      481 tgcttgttcc cacccgcgaa cttgccctgc aagtatccaa ggaagcccag atgttgacgc
      541 aagggacaaa tttgagaacg gcggtggttt acggcggagt aggttacaat gcacaactgg
      601 aagcatttcg cggcggcgct caacttgttg tgggcacacc gggacgaatc ttagatcatc
      661 tgctgaagaa tactctttct ctggatcacc tgaaaatatt gattttcgat gaagctgacc
      721 gcatgttatc aatgggattt taccctgata tggtgaaaat tcgtaaatat attccatcgc
      781 gtgaaatact cagcagcatg ttttccgcaa cttttccgcc gcaggtgatt cgtttagcag
      841 ctcaattcct gcgtcaacca caactactga gtttaagcgg caatcaggtg cacattgccg
      901 aaatagagca tatttattat gtcgtgccgg gtatgcgcaa agaaaggtcg ctggtgcgga
      961 ttattgaaat tgaaaacccg gcttccgcta tcatcttttg caataccaaa gacaccgtgc
     1021 attatctttc ggtagtcctg aagcggtttg gctatgatgc cgatgaattg agttccgatc
     1081 tggcccagag cgcgagggaa aaggtcatgg ctcgggtaaa gcgcggcgcc ctgcgttttt
     1141 tagtggcaac tgatgttgcg gccaggggta tcgacattcc cgatctttct cacgtaatcc
     1201 agtatgagcc gccggaggat ccggaggcgt atatccaccg tgccggacgt actggacgga
     1261 tgggatcaag tggtgtggcc atttctttag ttgcggaaat ggaaaaattc aagctgcaaa
     1321 gcattgcccg ttattataat attaatatgg aagagcgcca gcttgccagt gatgaagaat
     1381 tggaaagagt ggttgccgaa cgaataacgg ctcttttgga atcgcgtctg cgcgcccgcg
     1441 ataatcttca aattgagagg atgcaaagat tcttgccttt ggcgcgaaat ttagcgcagg
     1501 cggatgatga actggcgttg attgccatgc ttttggatga ttattatcaa caatcattgc
     1561 atgccccact ggttcaacca ttagctgtaa cagatatgac aattggtcaa catcaaaaat
     1621 caaagcagag aaggtcgcac cccgaaaata aaaaggaaag gaaaaaatag agaattatgc
     1681 agtttaattt tttccggtta cttaaaaata aaaaaagtat tcattttata tgtttcggca
     1741 tttttttatt tttgtttgtt tcctgtgctt caacatcgaa agaaattata ccgtcaaaaa
     1801 cggctaaaat tgctgttgtt ttaggtgccg gtgtgtctaa gggttttgct catatcggcg
     1861 ttttaaaagt tctggaatcg cataaaattc ccattgatat gattgtgggt accagtgtcg
     1921 gttcctttgt cggcagcctg tatgcttacg gtttcaatcc ctacgaattg cagactattg
     1981 cttttgccat tcaaaaagac gatgttgccg attatattat gcccgataat ggttttatca
     2041 aaggagaaaa actcgaaaac ttcgtcaaca ataaattgaa atatacacca atagataaat
     2101 ttaaaattcc cttctacgcc gtagccacaa atattcaaac cggcgaagag atggtattcg
     2161 gacggggaaa tgcagggaag gcagtgcggg caagct
//
LOCUS       DASVIM010000090         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_374600, whole genome shotgun sequence.
ACCESSION   DASVIM010000090 DASVIM010000000
VERSION     DASVIM010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374600"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VF305_01825"
     CDS             <1..403
                     /locus_tag="VF305_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415904.1"
                     /translation="SETGPFLVGSGGANDVASSANETVVTLEQNKNRFVEKVAYITSP
                     GVRVTTVISQLGILEKEAGNNELALTGYFPVNPSASEEESVRSIQEQCGWKLKVKSKL
                     QILPLPSAEELKLMRCFDPKRFFLKGDDSQR"
     gene            1099..>1805
                     /locus_tag="VF305_01830"
     CDS             1099..>1805
                     /locus_tag="VF305_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415905.1"
                     /translation="MENVVILSCARTPIGGYGGALKDIPVYRLGSFVLQEAVKRANVD
                     PVLVEDVIMGTAYQNGECANGARMAVLEAGWPDTVPGIVLDRRCCSGIDAIFFGAMKI
                     QTGNADIIIAGGMDSMSQTELYIPGDIKWGLGGKSHEKYGFMPRGHGALAMWGIPFYD
                     RIQRARVMSQPIARYGELSSMMSWAEAAAKKEKITREEADRWAYRTHQKAIAAQDAGK
                     FAAEIVPVPVGKGKTGEA"
BASE COUNT          551 a          359 c          437 g          458 t
ORIGIN      
        1 atccgaaaca gggccttttt tagtcgggtc aggaggggcc aatgatgtgg cctcgagcgc
       61 caatgaaacg gtcgttactt tggagcaaaa caagaatcgt tttgtggaaa aagtcgccta
      121 tataacgtca ccgggtgtta gagtaaccac cgtaatttct caattgggaa tattggaaaa
      181 ggaagcggga aataacgaac tggcattgac cgggtacttt cctgtaaatc catcggcatc
      241 ggaagaggaa tccgtgagga gcattcagga acaatgtggc tggaaattga aagtcaaaag
      301 caaactgcag attcttccct tgccatccgc agaagaatta aaactcatgc gctgtttcga
      361 tccgaaacgg tttttcctta aaggtgatga ctcacaacgc taggaatata aaacatttac
      421 tatatttgtc catcatcata catccaaatg cattggcggc ttaagattgt cgagtagttt
      481 cttcctcaag acttttagtg atgctggtag atatcgctct gaattgttat cgaacaattt
      541 gtgcagcaat ctgaaaccga agaaaaaaaa gcttaacaaa atttttcagt aattcaatta
      601 attttatcat attgaaaata taaggaaatt acatagcgct attcaagctt ttgtgaaaaa
      661 tatgaaaaat acttgataat gtatcttaaa ttgatatagg tataaaagtt tcatcaagcc
      721 gatatctttt gcacatatta gtgactctcg gtttttaaac gaatttttat attacacatg
      781 tccaaggagc aaatcaccat agacacgtgt aatcttaagg agtttttgaa agaaaaatta
      841 gaattccagg atcgcggtgg tacaggcaag attatagaat tctctgaggc agtacgacaa
      901 tttgtcagtc cggaaatgaa catacaaggt ggaaacgggc tggcgattca agaacaatgc
      961 tcctggaaat cgagctgctg ctaaacttaa tttatacgga aagaagactg gcgaattaaa
     1021 tcgaataaag gttggtatga ttacgctacc gctggaaata aactaataaa ctcgtaaaga
     1081 agagagtgag aaaaatatat ggaaaacgtt gttattctca gttgtgcgcg aactcccatc
     1141 ggcggctacg gtggggcatt aaaagacatt cctgtatacc gtcttggtag ttttgttcta
     1201 caggaagcgg taaaaagggc aaatgttgat ccagttctgg tcgaagatgt gatcatggga
     1261 actgcatatc agaatggtga atgcgccaac ggcgcgcgca tggctgttct cgaagccggc
     1321 tggccggata ctgttccggg gattgtcctt gaccgccgct gttgctcagg catagatgct
     1381 atttttttcg gggccatgaa aatccagacg ggaaacgcag atattattat tgccggcggt
     1441 atggactcga tgagccagac cgaactatat atccccggtg atatcaagtg gggtctcggc
     1501 ggcaaaagcc atgaaaaata cggcttcatg cccagaggac acggcgccct tgccatgtgg
     1561 ggaattccat tttatgatcg tatccagcgt gctcgtgtca tgtcgcagcc cattgctcgt
     1621 tacggcgagc taagctcgat gatgtcctgg gctgaggccg cagccaaaaa agaaaagatc
     1681 acccgcgaag aggccgaccg gtgggcatac cgcacccacc agaaggcgat cgcagctcag
     1741 gatgccggaa aatttgccgc agaaattgtc cccgtgcctg tcggaaaagg caaaaccgga
     1801 gaagc
//
LOCUS       DASVIM010000091         3459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_288666, whole genome shotgun sequence.
ACCESSION   DASVIM010000091 DASVIM010000000
VERSION     DASVIM010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288666"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..494)
                     /locus_tag="VF305_01835"
     CDS             complement(96..494)
                     /locus_tag="VF305_01835"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7415906.1"
                     /translation="MNNISNIKNERSSQNLTKKKIKKKALEMRNQGCLYDLPNEGLDD
                     YFNGSVDRFCDIAEKVRNKKRVLDVGCGQGVLLLILNELGHECHGVDIAEASDNLGER
                     FTQKNIKFQVGNIEVDSLPYTDGFFDAVVC"
     gene            complement(626..1477)
                     /gene="folD"
                     /locus_tag="VF305_01840"
     CDS             complement(626..1477)
                     /gene="folD"
                     /locus_tag="VF305_01840"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX7415907.1"
                     /translation="MAVIIDGNKIAQEIRQAVSKEALALKEKTGVVPGLAVILVGEDP
                     ASKVYVGRKAKACAEAGFLSREFKLPADTSEAKLLGIISELNADKLIHGILVQLPLPK
                     HIHTDKIIAAIDPRKDVDGFHPYNVGCLVSGNPLHVPCTPRGIMELISRTGIELIGKE
                     AVVVGRSNIVGKPIALLLLAQHATVTICHSRTKDLPAVIRRADVLIAAVGKTEMIKAD
                     MVKDGVIVIDVGVNRLENGKLAGDVAFTEVAAKASYITPVPGGVGPMTIAMLMRNTLN
                     AASGITS"
     gene            complement(1562..2092)
                     /locus_tag="VF305_01845"
     CDS             complement(1562..2092)
                     /locus_tag="VF305_01845"
                     /inference="COORDINATES: protein motif:HMM:NF021231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HEX7415908.1"
                     /translation="MKKAFAFVFFVLLWSAPFAWADELKVGEKAPNFSLQDYSGKVYT
                     LDSQEFQGRVISIFYNDPDKKNWNKHVEDALLKDDGLDRKNNYKGLRITNLKASKLPN
                     LIIKQVAKSKQKTTGSVILLDLDYSIIKAWGLKNYSSDVVVLDKERTCRYLYNGRLPP
                     EEVEKLISVIKEYQVK"
     gene            2363..>3459
                     /locus_tag="VF305_01850"
     CDS             2363..>3459
                     /locus_tag="VF305_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7415909.1"
                     /translation="MEESAHNYTNRTVLIFENQRINYDQLNRAVNAVAHLLKQTGVGK
                     GDKVALMLPNIPEFFYCYFAAVKLGAVAVPLNNLSTSYELLYLLGNSDAKVLMTVESA
                     RKRYEEIQDKLSTCKNIITVDNPDKNSTFRKALIAGDFSNPGEIISPDDPAAIIYTSG
                     LTGKSLGAVLTHRNLYSQSHLIHSCVKRTHLDVGLSLIPLFHSFGSTVNMINVIRTGC
                     CVVMMDRFTIEGLFSAIQKEKITYICAVPRLYLGMMFHESAANFDVSSLDVCVTGGAP
                     MPAEFIPAFEEKFGVKVVEGYGLTEASPACSFNMLDRPQKPGSIGMAIPGTKIKIVDE
                     NGRELPRGEIGELIVKGDNVMQGYYKDKAATA"
BASE COUNT          957 a          779 c          674 g         1049 t
ORIGIN      
        1 acacatctat ctctataatt ccgcccttcc tcaatactct atgcagctcc taatatagcc
       61 cagaggtgaa aatgtgtaaa gtgttcgaga acctgtcaac aaacaacggc atcaaaaaaa
      121 ccatcggtat atggaagtga atctacttca atattaccta cttgaaattt gatattcttt
      181 tgcgtaaatc tttcgcctaa attatcagat gcttcggcta tatccacgcc atggcattca
      241 tgaccgagct cattaaggat taacaataga actccctgac cgcagccaac atctaatact
      301 ctttttttat tcctaacttt ctcggcgata tcgcaaaatc gatctacgct gccattaaaa
      361 taatcatcca gtccttcatt gggcaaatca taaaggcaac cttgattacg catctcgaga
      421 gctttttttt taattttttt ctttgtaaga ttttgggatg atcgttcatt ctttatattc
      481 gatatattat tcattaaatt atactccacg atgccaatct ttatgattta gtctatccta
      541 actcaattct gaaacttaat ttcccctaag aaaagcttcg agaggaaaca aaggtgtatg
      601 ctttctactt tttttaatat gactgttaac tagttattcc tgaagcggca ttgagtgtgt
      661 ttctcatcag catagcgatt gtcatcggcc cgacacctcc cggcacaggt gtaatgtatg
      721 acgctttagc tgcgacttcg gtaaaggcta catctccggc cagcttgccg ttttccagac
      781 gattaacgcc cacatcaatg actatcacac catctttaac catgtctgcc ttaatcatct
      841 cagtttttcc cacggcggca attaatacat cggcgcgcct gattacagca ggcaaatctt
      901 tcgtgcgcga atggcagata gtaacagtcg catgttgcgc taaaagcaaa agggctatcg
      961 gctttccgac aatattgctg cgtcccacga ctaccgcttc ttttccgata agttcaattc
     1021 ccgtgcggct gattaactcc ataattcccc tcggtgtaca aggaacatgt aaaggattac
     1081 cggaaaccag acaacccaca ttgtaaggat ggaatccgtc aacgtctttg cgcggatcaa
     1141 tggcggcaat gattttgtcg gtatggatat gtttgggtag agggagctgc accaaaattc
     1201 cgtgaataag tttgtccgca tttaattcgc tgataatgcc caatagcttt gcttcacttg
     1261 tatcggcggg aagcttaaac tcgcgcgaca agaaaccggc ttccgcacaa gccttggctt
     1321 ttctacctac atagactttt gatgcagggt cttctccaac gagaattacg gcaagtcccg
     1381 gcacaactcc tgttttttct ttcaacgcca gagcctcctt gcttaccgct tggcgaattt
     1441 cctgcgctat cttgttacca tcaataatta cggccataaa tcctcctgtt tacaatatta
     1501 aaatttctca ccgtttatca gaaaatactg gtttaacgga aaaaatataa attcagtaag
     1561 tttattttac ttgatattcc ttaatgacgc taattaattt ttccacttct tccggcggaa
     1621 gcctgccgtt gtaaagatag cggcaggtcc tttctttgtc caaaacaaca acatcggaag
     1681 agtaattttt aagtccccac gcctttatta tgctgtaatc caaatcaagc aatataaccg
     1741 agccggtcgt tttctgtttg ctttttgcta cctgtttaat aattaaattg ggtaatttgc
     1801 tggctttgag attagtaata cgtaagccct tataattgtt ttttctatcc agcccatcgt
     1861 ctttgagtag ggcgtcttca acgtgtttgt tccagttttt tttatcggga tcgttataaa
     1921 aaatagaaat gacccgtccc tggaattctt gtgaatcgag tgtataaact ttgcccgaat
     1981 aatcttgcag agagaaattg ggggcctttt cgccgacttt caactcatcc gcccaggcga
     2041 aaggggcgct ccataataaa acaaaaaaaa caaatgcaaa agcctttttc atttgatctt
     2101 ccttttaatc gcccgtaagt caacttagat atgtcgagtt tgaggcaact taatatgagc
     2161 aggttgctga gtttataaaa atcacggcgg attataggat aaaacatcca tacaggcaag
     2221 cgaaaaactg tttttcccaa cgcgggcaaa tacggccccg gctttcatta tcttgttgaa
     2281 aaaatgtgcc ttattgtgct aaacgaattt tcaaatttca aatttcgcgg aggtttttga
     2341 gactattaat ctagcactct tattggaaga atctgcccat aattacacca accgaacggt
     2401 tctgatcttt gagaatcaac gtattaatta tgatcagttg aatcgtgccg tcaacgcggt
     2461 tgcgcatctt ttaaaacaaa caggtgtggg gaaaggcgat aaagttgctc tgatgcttcc
     2521 taacattcct gaattttttt actgttattt tgccgcggta aaactgggtg ctgtcgctgt
     2581 tcccctgaat aatttatcca cgtcttatga gttactatat ctgctgggaa acagcgatgc
     2641 taaagtatta atgacagtgg aatcggcacg aaaaaggtat gaagaaatac aagataagct
     2701 ctccacctgc aaaaacataa tcacggttga taatcccgat aaaaattcta ctttccggaa
     2761 ggcgcttatc gcaggagatt tttccaatcc gggggagata atatctcctg atgatcccgc
     2821 cgctataatt tatacctccg gtcttaccgg caaatcttta ggagcggttc ttacccatcg
     2881 caatctgtac tctcagtctc atttgataca ttcttgtgta aaaagaactc atctggatgt
     2941 aggactttcc ctcatccctt tatttcactc ttttggctca accgtcaaca tgatcaatgt
     3001 tattcgtaca ggatgctgtg tagtcatgat ggatagattc acgatagagg gcctttttag
     3061 tgccattcaa aaagaaaaga tcacgtacat atgtgccgta cctcgtcttt atctgggcat
     3121 gatgtttcac gagagcgcgg ctaatttcga tgtcagctct ctggatgttt gcgttaccgg
     3181 gggcgcgccg atgccggccg aatttattcc cgctttcgaa gaaaaattcg gcgtaaaagt
     3241 tgtggaaggt tatggtttaa cagaagcctc accggcttgt tccttcaata tgctcgacag
     3301 gccgcaaaag ccgggttcca tcggcatggc catccccggc actaaaatta aaattgtcga
     3361 tgaaaacggc cgggaactgc cccgtggtga aatcggcgaa cttatcgtaa aaggcgataa
     3421 cgtcatgcaa ggctattata aggacaaagc ggcaacggc
//
LOCUS       DASVIM010000092         6967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_543741, whole genome shotgun sequence.
ACCESSION   DASVIM010000092 DASVIM010000000
VERSION     DASVIM010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543741"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VF305_01855"
     CDS             <1..251
                     /locus_tag="VF305_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415910.1"
                     /translation="GEFFLRLEKNSKIAVISMEITLKDIKQNKANKRIVFQKKYHDEQ
                     LLTEQSPRGYCQAMSHALRVLSQQITTDVYQAIKTSEN"
     gene            270..668
                     /locus_tag="VF305_01860"
     CDS             270..668
                     /locus_tag="VF305_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX7415911.1"
                     /translation="MKEIDIRTNSRTEMIDITNLVQVAINEEKVKSGICVVFAPHTTA
                     AVTINENADPDVPRDIISALEKTVPKSANYRHTEGNSPAHVKSSLLGASELVLIEDGR
                     MVLGTWQSIFFCEFDGPRQRKVLVSVLANK"
     gene            complement(708..1490)
                     /locus_tag="VF305_01865"
     CDS             complement(708..1490)
                     /locus_tag="VF305_01865"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX7415912.1"
                     /translation="MLQWKIFAVDLARKAGVLLKEKFSQKHDIHYKGEINLVTEADKM
                     SDDLIITAIRQTFPDHGILSEESPAIVGGGKFRWIIDPLDGTTNYAHGYPVFCVSIAL
                     VKEDAIVLGVIYDPLREEMFVAVRGEGAFLNGKKMAVSPTRDISRSLLATGFPYDIRE
                     SKENNLDYFNVMAKEVQAIRRAGAAALDIAYVASGRFDGFWELKLMPWDMAAGCLMVE
                     ESGGVVSGLFGERWSMSSPHVLVSNGLIHEQMIAVFKKTVQK"
     gene            1647..2555
                     /locus_tag="VF305_01870"
     CDS             1647..2555
                     /locus_tag="VF305_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX7415913.1"
                     /translation="MVIEKKPSNNSQDLKATREAQGLSLKDVFRSTRVSVVYLQALEN
                     SDFYLLPAPIYTKNFIKTYARALGIDSKPILTIYEDYLNSLKVLQLLPPEDILEGRHL
                     SARIASYKTYWGIISVLIVIGVVVWLISKQYQPASDIIYSTGRIVAVTPENMEQTVNS
                     PVNAADSINQQIKTSSPLALNEINKKSQTQAQSISAGGKNNAVKPLEEQSSSTVENVQ
                     PRPIANSEEAGVLVIKAIEETWIRIKADQNPSFQVLLKTGEKIERKAATFDMDIGNAG
                     GIKIQFKGKNIENLGNSGQVIHLRLP"
     gene            2588..2773
                     /locus_tag="VF305_01875"
     CDS             2588..2773
                     /locus_tag="VF305_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX7415914.1"
                     /translation="MFGIGMPELLIILVIILIIFGVGKLPEIGSALGKGIRNFKKSMN
                     ENSEISNQAAKKEEEKK"
     gene            complement(2932..3345)
                     /locus_tag="VF305_01880"
     CDS             complement(2932..3345)
                     /locus_tag="VF305_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX7415915.1"
                     /translation="MVNKAILIGRLGKDPEVRYTPDGTMVTNFNLATDEQWKDKNGEK
                     VQKTEWHRIVTFGKLAEICGNYLVKGKLVFVEGRIQTRSWEDKDGVKRFTTEIVAANM
                     QMLDSKGQNKTDESSSQAAPVSPHNDNTPADDVPF"
     gene            3528..5009
                     /gene="lysS"
                     /locus_tag="VF305_01885"
     CDS             3528..5009
                     /gene="lysS"
                     /locus_tag="VF305_01885"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX7415916.1"
                     /translation="MEDNDLLKVRLEKIAALKAAGVDLYPNDVKPQNTTAELFAQFGN
                     SDAEALRQSAKRFSIAGRLMAVRNFGKAAFVKIQDRKGQIQCYVAKDILSADVFFIFK
                     KLDIGDIIYISGKLFRTKTNELTIEAEDLRLLSKAIHPLPEKWHGLTDIETRYRQRHL
                     DLITSPKVKEIFYRRSHIIKLIRQFMDKHDFLEVETPMMQPRAGGAMARPFKTHHNAL
                     DMDFYLRIAPELYLKRLVTGGLERVYEINRNFRNEGISTFHNPEFTMMEFYLSYATYE
                     DLMSYTEELFTFIAMNTFDGLKFTYQGTEIDLTLPWRRISVKDALLQIAGMEPSELDN
                     LPKALAFARKVGCDVKETYPLGKILMAIFDEVVEKKLIQPTFVTHYPVDVSPLSRRSD
                     NDPAVVDRFELYIYGREIANAFSELNDPADQRERFLQQLQEREAGDDEAHEMDEDYIK
                     TLEYAMPPTAGEGIGIDRLVMLFTDSASIRDVILFPLLRTKQE"
     gene            5073..6356
                     /locus_tag="VF305_01890"
     CDS             5073..6356
                     /locus_tag="VF305_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX7415917.1"
                     /translation="MPYEFFISKRYLRAKRKQVFVSIITFISIFGIFLGVAALIIVLA
                     VMNGFEEDLRSKILGIKSHIELTTDMAGPMKNYQEVSERITGMEGVVASTPFIYSQAM
                     IRSDNGVTGVVIRGLDTQSAFKVINLGKIREGKIEYLNKIPDEILKHYKKEDAQAAGI
                     VIGKEMARNLGIFLYDSITIISPVGISTPMGMVPRMKKFVVVGIFESGFYEYDSTLAY
                     LSLKNCQDFLQMGDTVTGIDIKVDDIYKADSIARKIQSKLGFPFWAHNWMQMNKNLFS
                     ALKLEKRVMFIILSLIVLVAAFNIISALIMIVMEKNKDIAILKSMGATSRTIMKIFIY
                     QGLIVGVIGTFSGCVAGLAVALNLQKISLLVERIFHFKILPGDVYYLSELPSKVNYGD
                     VVVIVIGTLLICFLSTIYPSWRASKLDPAEALRYE"
     gene            6381..>6967
                     /locus_tag="VF305_01895"
     CDS             6381..>6967
                     /locus_tag="VF305_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7415918.1"
                     /translation="MIILENLSKTFIKDGNKIEVLRGLNLEIAKGESLAILGVSGAGK
                     STLIHILGTLDHPTEGSLSINGLNVFEWGEKKTARFRNSTIGFVFQFNNLLPEFDALE
                     NTMMPALISGMSKKQAGEKACKLLAEVGLEHRLKHKPGELSGGEQQRVAIARALVMEP
                     GILLADEPTGNLDTETGKKIEDILVNLNESKKITLV"
BASE COUNT         2182 a         1393 c         1395 g         1997 t
ORIGIN      
        1 tgggagaatt tttcctgcgc ctagaaaaaa acagcaagat tgccgtcatc agtatggaga
       61 taacgctaaa agatatcaaa caaaataaag caaataaacg tatagtcttt caaaaaaaat
      121 accacgatga acaattgctg accgaacaat caccgcgtgg gtactgccag gcaatgagcc
      181 atgcgttgag agttctttcc caacaaatta ccaccgatgt ttatcaggcg attaaaacct
      241 ctgaaaatta attttccagc gagaatgtca tgaaagaaat cgatattcgg acgaattcgc
      301 ggacggaaat gattgatatt acgaatttag tccaagtggc gatcaacgag gaaaaagtta
      361 aaagcggcat ttgtgtcgtt tttgcgccgc atacaactgc cgcagtcact atcaatgaaa
      421 atgccgatcc cgacgtgccc cgtgatatca tttctgcctt ggaaaagact gtcccaaaaa
      481 gtgctaatta cagacatacc gagggaaatt ctccggcaca cgtaaaatca tcactgctcg
      541 gtgcttcgga gttagttctc attgaagacg gccggatggt tttaggaacc tggcaatcaa
      601 tatttttttg tgaatttgac ggtccccgac aaaggaaagt cctggtttct gttcttgcca
      661 acaaatagat tttcaaacga tgcgcagcaa aacatttttt cacagcttta cttttgaact
      721 gtctttttaa acactgcaat catttgctca tgaatcaagc cgttggagac aagaacatgt
      781 ggcgaagaca tactccatct ctcgccaaat aagccggaaa caactccgcc ggattcttcc
      841 accatcaggc agccggcagc catatcccag ggcataagct ttaattccca aaagccatcg
      901 aagcggccgg aagctacata agcaatatcc agcgcggcgg ctccggcgcg gcggatagct
      961 tgtacctctt tagccattac attgaaataa tcaagattgt tttctttgct ttcccgaata
     1021 tcatagggaa aacctgtagc cagaagactg cgggaaatat cacgcgtcgg cgaaacagcc
     1081 atcttttttc cgtttaaaaa cgctccttcg ccgcggacag caacaaacat ttcctccctt
     1141 aacggatcgt aaattacacc caaaacaatt gcatcttctt tcactaaggc aatagaaacg
     1201 caaaaaacag gatacccatg agcataattc gttgttccat ctaagggatc gattatccaa
     1261 cgaaactttc caccaccaac tattgccggt gactcttccg ataaaatccc gtggtcgggg
     1321 aatgtctgtc tgatcgccgt gataatcaaa tcatcggaca ttttatctgc ttcggtaacg
     1381 agattaatct cgcctttata atgaatatca tgcttttgac tgaacttttc ctttaataac
     1441 acaccggcct ttcgcgccaa atccactgca aatattttcc attgcaacat ataccaatac
     1501 tcctattata atccttgatc atagtaataa aattcaaaag aaatataaag aaataatgta
     1561 acgataagct tcgttttaaa aagttgcgct acaataaaat taatgctaaa cggacacaaa
     1621 taaatgtcgt tatttttgat ttgtatatgg ttatagaaaa aaaaccaagc aataattcac
     1681 aagacttaaa agcaacaagg gaagctcagg gtctttccct taaagatgta ttccggagca
     1741 cgcgcgtgag cgttgtctat ttgcaagccc tagaaaatag cgatttttat ctgctgccgg
     1801 ccccgatcta cacaaaaaat ttcattaaaa cttacgcccg tgctttaggt attgacagta
     1861 aaccaatact taccatctac gaagattatc ttaactcttt gaaagtttta caattactgc
     1921 caccggaaga tatcctggag ggaaggcatc tttccgcgag aatcgccagc tataaaactt
     1981 attgggggat tatctccgtt ttaatagtta ttggtgttgt ggtctggtta atttctaaac
     2041 aatatcaacc agcttcagat atcatttact ccacggggag aatcgttgct gttaccccgg
     2101 aaaatatgga acaaactgta aattcgccgg ttaatgcggc tgattccata aaccagcaga
     2161 taaaaactag ttccccgctt gcgctaaacg aaattaataa aaaatcacaa actcaggcgc
     2221 aatcaatttc cgccggggga aaaaataatg ccgtaaaacc tttagaagag caaagctcgt
     2281 caacggttga aaatgtgcag cccaggccca tagctaatag tgaagaagcc ggcgtcctgg
     2341 ttattaaagc tatcgaggaa acatggataa gaattaaagc tgatcagaat ccctcgtttc
     2401 aggttttgct gaagaccgga gaaaaaatcg agcgcaaagc agcaactttt gatatggata
     2461 ttggcaacgc cggcggaatt aagatacaat ttaaaggtaa aaatatagaa aacctaggca
     2521 attcgggaca ggtaatccat ttgcggttac cataattttg aagaataaaa attaatgagg
     2581 taatattatg tttggaattg gtatgccgga attactgata atattagtaa ttattctgat
     2641 tatctttggc gtagggaaac tgccggagat cggttcagcg ctgggcaaag gaataagaaa
     2701 ctttaagaaa tcgatgaacg aaaacagcga aatttccaat caagcggcaa aaaaagaaga
     2761 agaaaaaaaa tagcctctac caaataaaaa aacacgattc aacaattcgg gcttttttgc
     2821 actaaaccgc gctcctttta tcttcaggga gaactttata gaaccttgtt ttatgtaatt
     2881 tactttaatc ttaccggcaa ggctcaatga aagtgcagta tccgttgccg gttaaaatgg
     2941 aacatcatct gccggagtat tatcattatg tggacttacg ggtgctgcct gggaagatga
     3001 ttcgtctgtt ttgttctggc ctttggaatc gagcatttgc atattggcag ctacgatttc
     3061 cgtagtgaag cgtttaacac catccttatc ttcccacgaa cgagtttgaa tccgcccttc
     3121 cacgaaaacg agtttgcctt taacgagata gttaccgcat atttcagcta actttccaaa
     3181 agtaactatt ctatgccact ccgttttttg aactttttcc ccgtttttat ctttccactg
     3241 ttcgtccgtg gccaaattga aatttgtaac cattgtgcca tcgggcgtat aacggacttc
     3301 cggatcttta cccagtctgc cgatcaatat tgccttgttt accattttac tctcccccta
     3361 ttgataagtc ttcgctataa tacataacat catcatttgt agcaaactaa ttttgtgtag
     3421 aaaatttaaa ttaaagcatt atttacacta taactattat acaatcatgc ttgactattt
     3481 gaaggaaaaa atatattcaa acacgcttta taaaaagtgg agaaattatg gaagataatg
     3541 atcttttaaa ggttcgcttg gaaaaaattg cggccttaaa agcggccgga gtcgatttat
     3601 atcccaatga cgtgaaaccc caaaacacaa cagcagaatt atttgctcaa tttggcaatt
     3661 ctgatgctga agcgctgaga cagtcagcaa aaagattctc tatagccggc cgtttaatgg
     3721 ccgtgcgtaa ttttggcaaa gcagcgtttg tcaaaattca agaccgcaaa ggccaaattc
     3781 aatgttacgt agccaaagac attcttagcg ccgatgtttt ttttattttc aaaaaactgg
     3841 atattggtga tattatttat atttccggga aactctttcg cacaaaaacc aacgaattga
     3901 ccatagaggc cgaagattta cgcctgttat ccaaggccat ccatcctctt ccagaaaagt
     3961 ggcatggcct taccgacatc gaaacaagat accgccagag gcatcttgat ttgattacca
     4021 gtcccaaggt taaagaaatt ttttaccggc gcagccatat tatcaagctt attcggcagt
     4081 ttatggacaa acatgatttt ctggaagtgg aaacgcccat gatgcagccg cgtgcaggtg
     4141 gtgcaatggc tcgtcccttt aaaactcatc ataacgcgct ggatatggat ttttatctgc
     4201 ggattgctcc tgaactttat ttaaaacgtc tcgttaccgg cggactggaa agagtctatg
     4261 aaattaaccg gaacttccgc aatgaaggca tttccacctt tcataatccc gaatttacaa
     4321 tgatggagtt ttacttaagt tacgccactt atgaagattt aatgtcctat acggaagagt
     4381 tatttacctt tatagctatg aatacattcg atgggcttaa gttcacttat caaggaacag
     4441 aaattgattt aaccctgcct tggcggcgca tctctgtaaa agatgccctg ttacaaatag
     4501 cgggtatgga accttccgaa ttggacaatc tccccaaagc tttagctttt gccaggaaag
     4561 ttgggtgtga tgttaaagag acatatccgt taggaaaaat attaatggcc atttttgacg
     4621 aagttgtcga aaaaaagctt attcaaccta catttgtaac ccattatcca gtagacgtat
     4681 ctcccctttc ccgcagatca gacaatgatc ctgctgttgt tgaccgtttt gagctgtata
     4741 tttacggcag ggaaattgct aatgccttct cggagttaaa cgatcctgcc gatcagagag
     4801 aaagatttct gcagcaatta caggaaagag aagctggtga tgacgaagct catgaaatgg
     4861 acgaagatta catcaaaaca ttggaatacg ctatgccacc tacagccggc gaaggtatcg
     4921 gtattgaccg gctggtaatg ctttttaccg attcagcatc tattagagat gtcattttat
     4981 ttccgctatt gaggacaaaa caggaataaa attgagatat taaccaattt ttttgaaaaa
     5041 ctaaattgcc cacaaatgca ggatatattt taatgcccta tgaatttttt ataagtaaac
     5101 gttatttaag agcaaaacgc aaacaagtat ttgtttcgat catcacgttt atatcaatct
     5161 tcggtatttt tttgggtgtg gccgccttga tcatcgtatt ggctgttatg aatggatttg
     5221 aagaggactt gcgttcaaaa attttaggaa ttaaatctca tattgaattg actacggata
     5281 tggccggccc catgaaaaat tatcaagaag ttagcgaacg aatcaccggc atggaaggag
     5341 ttgtcgcttc cacaccattc atttacagtc aggcaatgat tcgcagcgat aatggcgtta
     5401 ccggtgtagt tatccgcggc ttggatactc aaagcgcttt taaagtaatc aatctgggaa
     5461 aaattcgaga aggaaaaatt gaatatctaa acaaaattcc ggatgaaata ttaaagcact
     5521 acaaaaaaga agatgctcag gcggccggga tcgtcatagg taaagaaatg gccagaaatt
     5581 tgggcatttt tctttacgat tctatcacca ttatttcacc ggtgggtatt tctacaccca
     5641 tgggaatggt tccgcgcatg aaaaaatttg ttgttgtggg tattttcgaa tcagggtttt
     5701 acgaatatga ttcgacgcta gcttatttat cattgaagaa ctgtcaggat ttcttgcaaa
     5761 tgggcgatac tgtcacggga attgatataa aagtcgacga tatttacaaa gcagatagca
     5821 ttgcccgaaa aattcaaagc aaactgggtt ttcccttttg ggcccataac tggatgcaaa
     5881 tgaataaaaa tcttttttcc gctttaaagc tggagaaacg cgtcatgttt attattctga
     5941 gcttaattgt tctggttgcc gcgtttaata taatcagcgc attgatcatg attgtcatgg
     6001 aaaaaaacaa agacattgcc attttaaagt ctatgggggc aacttcccgc accattatga
     6061 aaatatttat ttatcaaggt ctgattgtcg gtgtcattgg cactttttca ggttgtgttg
     6121 ctggacttgc tgttgcttta aatttgcaaa aaatatcgct tcttgtggaa agaatttttc
     6181 attttaagat attacccggc gatgtttatt acttaagtga acttccatct aaggttaact
     6241 acggagacgt tgttgtcatt gttattggta cgctgcttat ttgcttttta tcaacaattt
     6301 atccttcttg gagagcttca aagttagatc cggctgaagc cttgagatac gaataaaatt
     6361 aagatcgcga atgtaaaatc atgattatac tggaaaattt gtcaaaaaca tttattaaag
     6421 acggcaataa aattgaagtt ttgcgtggcc tgaacttaga aattgccaag ggtgaatctt
     6481 tggcaattct tggcgtttcc ggtgccggca aaagtacact cattcatata ctgggcacgt
     6541 tggatcaccc caccgaaggt agtctttcca ttaatggcct gaacgttttt gagtggggag
     6601 agaaaaaaac tgctcgtttc cgtaactcaa cgattggttt cgtttttcaa tttaataatt
     6661 tgttgcctga attcgatgct ttggaaaata ccatgatgcc ggctttaatt agcgggatgt
     6721 ccaagaaaca agccggagaa aaagcttgca aattacttgc tgaagttgga ttagaacacc
     6781 gattaaagca taagccggga gaactttccg ggggagaaca acagcgcgtg gctattgccc
     6841 gggccttagt catggagccg ggaatattac ttgccgacga acctacggga aatcttgaca
     6901 cggaaacggg aaaaaagata gaagacatac tggttaattt aaacgaatca aaaaaaatta
     6961 cccttgt
//
LOCUS       DASVIM010000093         4654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993 k141_34765,
            whole genome shotgun sequence.
ACCESSION   DASVIM010000093 DASVIM010000000
VERSION     DASVIM010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34765"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VF305_01900"
     CDS             complement(<1..461)
                     /locus_tag="VF305_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEX7415919.1"
                     /translation="MNILIFGPNGSGKGTQGAVVQKKYGIPHIETGVIFRQNISQKTP
                     LGEKAKAFIDRGELVPDDITVPMILDRLKENDCKKGWLLDGFPRNLAQAEALWKTLEK
                     EKIKLDYVIEIDLDRETAKKRIMGRRLCQKDNNHPNNIYIDAIKPHEHEGKT"
     gene            complement(883..2304)
                     /locus_tag="VF305_01905"
     CDS             complement(883..2304)
                     /locus_tag="VF305_01905"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7415920.1"
                     /translation="MRKLIQNQYIFFAVAFFILFFRTSLCFAEFTIDDEKKLGKEFYD
                     KLEKNHFLLENKRLNDYITKIGNLVLMQSKKAPFDFHFLIVNSSAINAFATPGGYIYV
                     NKGLINAVENEGELAGVIAHEIGHANARHVASIIEKSQKLSIATLAAIIAGAFLGGGG
                     EATAAIAAFSVAGASSLTLKYQREHEEEADRLGIEYLVSAGYYPAAMVDFLKIIKKYD
                     FFSKSIPSYLRTHPGTDERIFYLDSLILTEYHQSGAKNIVGNFSRMQALLSLNMDDLN
                     SRQKQLKEDLSNKPQNVDLLYALALTEDQLGQTSSALEHYQKALSLSPRDEDVLKNIG
                     LIYLKLGKADLAQGYLLRVVNLNEDNDEVIFALGNTYFALGNYQSALNSYLKLKDKVL
                     DDADINYYIAMAYGKLNNQGESHYYFGLHFKKSKKKESALFHFEEALNYFPQGSERSV
                     IINNEIKELSIDSPQQSKSKSKH"
     gene            2421..3089
                     /gene="cmk"
                     /locus_tag="VF305_01910"
     CDS             2421..3089
                     /gene="cmk"
                     /locus_tag="VF305_01910"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX7415921.1"
                     /translation="MEKKFILTIDGPAGAGKSTVSKALAKKLDYIYLDTGALYRALAY
                     KALKVKISVDNTSALANLCSSTNVVLKNIAGQIKVYVDGEDVEDKIRTEEVGLAASKI
                     STFAVVRDSLLNLQREAGAHGGIVAEGRDMGSVVFPHADYKFYLDADVEERIKRRHKE
                     LLAKGKSIEYEAIQKDMLARDSQDSKRQIAPLKATADAIIIDSTNLSVPEVVEKIIRY
                     ISLK"
     gene            3208..>4654
                     /locus_tag="VF305_01915"
     CDS             3208..>4654
                     /locus_tag="VF305_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX7415922.1"
                     /translation="MVNNNNINLTKERKVEAESSAKGTVIQSSQPKEEDMGFRELYEQ
                     SLNQLQYGDVARGKVVQIKDDVVMVDVGWKTEGFIPIGELKDAQGNINISVGDDIEVF
                     IDKRDSDGNLILSKDKASKLKIWDEIKNACENNIPIKGTVVEKVKGGLSVNIGIFAFL
                     PGSQVDIRPVKDLDKFVGKILDFNVLKYDRKRNNVVLSRRSIVASEKEIEKKDILSSI
                     QEGNVVEGVIKNITDYGIFIDLGGIDGLLHVTDISWGRIAKPAESFHKGEKITVKVLS
                     FDREKERVSLGLKQLTDNPWEHIVEKYPVGSIAEGKVVNLTDYGVFVELEPGVEGLVH
                     ISEMYWTREIKHPSKVLNISEKINVMVLEVNPEAKRVSLSLKQTAPNPWEKLKEKYPV
                     GTTVTGIVRNITNFGVFVGIEEGIDGLVHVSDISWKHRVTHPSEYFKKGQEVEAIVLN
                     VDVENEKFSLGIKQIEKNPWEDLSQKYAPGSV"
BASE COUNT         1494 a          795 c          967 g         1398 t
ORIGIN      
        1 gtttttccct cgtgttcatg aggtttaatc gcatcgatgt agatattgtt gggatggtta
       61 ttatccttct ggcacaggcg gcgtcccata attcgtttct ttgccgtttc acgatcgaga
      121 tcaatttcaa taacgtagtc caatttgatt ttttcttttt ccagtgtctt ccagagagct
      181 tcagcctgag ccaggttgcg ggggaaacca tccagcagcc agcctttttt gcaatcattt
      241 tccttcaggc gatccagaat catgggaacg gtaatatcat caggcacaag ctcgccgcga
      301 tcgataaatg cttttgcttt ttctcccagc ggagtttttt gagaaatatt ttgccggaaa
      361 ataactccgg tttcaatgtg gggtatgcca tatttctttt gtactactgc gccttgagtt
      421 cctttgccac tgccgttggg accaaaaatc aaaatgttca cttttagact cctttcctga
      481 aaaatattaa agttggagtc ttataatgta ttgtgtgctt tttcgcaatg ataaaagaat
      541 aatcgaaaat cccttttaaa atcaaagggg aaatatttta atggcagtaa cctgaagaac
      601 aacttggcga gcaggtggtt gatgagtatg tggaacaatt tgacttttcc ccggcaaata
      661 ccgacattat tttttgacta ttttttgctc cgcattccgg gcaacaaagc tttttttctt
      721 ccgctggtga aaaaattaaa ccttcaaaga catttttaca ctctttgcat ctaaattcgt
      781 aaatcggtat agtttgtttg tcctttaaaa taggcagaga cagcatgaat gtcaaattta
      841 aactctaatc aaattcatac tggcaatatg aaagaacaat tttcagtgct ttgatttgct
      901 ttttgactgt tgaggtgagt caatacttag ttctttaatt tcattattga ttataacgga
      961 tcgttccgaa ccttgtggaa aataattaag agcttcttcg aaatggaaga gggcgctttc
     1021 cttcttcttg gattttttaa aatgtaggcc gaaatagtag tgtgactcac cttgattatt
     1081 gagcttaccg taagccatcg cgatgtaata attaatatca gcatcatcaa gcaccttatc
     1141 tttcaacttt aggtagctat taagagcact ttgataatta cctaaggcaa aataggtatt
     1201 acccaaagca aaaataactt catcgttgtc ttcatttaaa tttacaacac gcagaaggta
     1261 gccctgagca agatcagctt ttcccaattt cagataaatt aagccgatat tttttaaaac
     1321 atcttcatcc cgcggagaca aactcagcgc tttttgatag tgttcgaggg ctgaacttgt
     1381 ttgtccaagt tgatcttcag ttaaagcaag agcatagagc aaatcaacat tctgaggctt
     1441 gttactcaaa tcctctttga gttgtttctg ccgggaattc aaatcgtcca tatttaatga
     1501 aagcaaagct tgcattctgc tgaaattacc cacgatattt tttgctccgc tttgatgata
     1561 ttctgttaaa atcaaactat ctaaatagaa aatgcgttca tcggtacccg gatgcgtacg
     1621 caaataggaa ggtatgcttt tggaaaaaaa atcatatttt ttaatgatct taagaaaatc
     1681 aaccatggca gccggataat aaccagcgct gacaagatat tctattccca ggcggtctgc
     1741 ttcttcttca tgctcccgct gatatttcag cgttagacta ctggcgccgg caacagaaaa
     1801 ggcggcaata gccgcagttg cctcgcctcc gccgcccaga aaagcgccgg caataattgc
     1861 ggctaatgtg gcaatgctta atttttgtga tttttcaata atgctggcaa catggcgggc
     1921 attggcatgg ccgatttcgt gagcaataac tccggccaac tcgccttcat tctccacagc
     1981 gttaattaaa cctttgttaa cgtaaatgta accgccaggc gtggcaaatg cgtttattgc
     2041 tgaactattc acgatcaaaa aatgaaaatc aaagggagct tttttacttt gcattaaaac
     2101 taaattgccg attttggtta tgtagtcatt aagacgttta ttttcaagga gaaaatgatt
     2161 tttttccaat ttatcataaa attctttgcc cagttttttt tcgtcatcaa tagtgaattc
     2221 ggcaaagcaa aggcttgtcc taaaaaataa gataaaaaat gcaacagcga aaaaaatata
     2281 ttgattttga ataagtttgc gcataagcag ttttcgaatt aaatggtata taatcttact
     2341 aataccttct ttataatttt aaggcaagtt ttcctttttt cttaaggtca actttaagat
     2401 tttagcgagg aataacatgt atggaaaaga aatttatttt aactattgat gggcccgccg
     2461 gtgccggcaa aagtacggta agcaaagctt tggcaaaaaa gctggattat atttatctgg
     2521 atactggagc actttatcgg gcgctggcgt ataaagcatt aaaagttaaa atatctgtgg
     2581 ataatacatc agcattggct aatctgtgtt ccagcaccaa cgtggtactg aaaaatattg
     2641 ccggccaaat aaaagtatat gtagatgggg aagatgttga ggataaaata cgcactgaag
     2701 aagtaggttt ggctgcttcc aaaatatcca cttttgccgt cgtccgagac agtttgctta
     2761 atctgcaaag agaagcagga gctcatggcg gtatcgttgc agaaggaaga gacatggggt
     2821 cggtagtatt tccgcacgct gattacaaat tttatcttga tgccgatgta gaagaaagaa
     2881 taaaaagaag gcacaaagaa cttttagcta aaggtaaatc aatcgaatat gaagcaattc
     2941 aaaaagatat gctggcaagg gatagtcagg attcaaaacg gcaaattgca cccctgaaag
     3001 caaccgccga tgcaataata attgactcaa cgaatttgtc cgtgccggaa gtagtagaaa
     3061 aaataatccg gtacatttca ttaaaatgag gcgtgggaaa atattacagt aaagaaaaat
     3121 gtaatggatt tatgttgcta tttaattttc agtatgttaa tttttaacga ttatagagct
     3181 tcataaaaat tagggggtat ttgtttaatg gttaacaata acaacataaa cttaacaaaa
     3241 gaaaggaagg ttgaagcgga atcttcagct aaaggtacag taattcagtc atctcaaccc
     3301 aaagaagaag atatggggtt cagagagcta tacgaacaaa gccttaacca actgcaatat
     3361 ggagatgtcg ccagaggcaa agtggtacaa atcaaagacg atgtggtcat ggttgatgtc
     3421 ggctggaaaa ctgaaggttt tattcccata ggagagctta aagacgcaca agggaatatt
     3481 aatatttccg ttggcgatga tatagaagtg tttattgata agagagattc agacggcaac
     3541 ttgatcttgt caaaagataa ggcatccaaa ttaaaaattt gggatgaaat taaaaatgca
     3601 tgcgaaaata atatcccgat aaaaggtact gttgtcgaaa aagttaaagg gggcctttcc
     3661 gtcaatatag gtattttcgc tttcttgccc ggttctcaag tagatatccg tccagttaaa
     3721 gatttagata aatttgtcgg caaaatatta gattttaacg tgttaaaata tgaccggaag
     3781 cgcaacaatg tagttttgtc gcgccgttcg attgttgctt cagaaaagga aatagaaaag
     3841 aaagatattt taagctctat tcaagaaggc aacgttgtag aaggtgtgat taaaaatatc
     3901 accgattacg gtatttttat tgatttggga ggcattgacg gactgcttca tgtgacggat
     3961 atttcctggg gcagaattgc taaacctgca gagagtttcc ataagggtga aaagattacg
     4021 gtcaaagttc tttcctttga ccgtgaaaag gagagagttt ctttaggtct gaagcagtta
     4081 actgataatc catgggaaca tattgtagaa aaatacccag tcggctcaat tgctgaaggt
     4141 aaagtcgtaa atttgactga ctatggtgtt tttgtggaat tggaacctgg agtcgaagga
     4201 ttagtccata tatcggaaat gtactggaca agagaaatta aacatccgtc gaaggtattg
     4261 aatataagcg agaaaatcaa tgttatggtt ttagaagtaa atccggaagc gaaaagagtt
     4321 tcattaagct taaaacaaac agcgccaaat ccctgggaaa aactaaaaga gaaatatccg
     4381 gtagggacaa ctgttacagg tatagtcaga aatattacta attttggagt ttttgtaggt
     4441 atcgaagaag gaatcgatgg attggtgcat gtgtctgata tttcatggaa gcacagagtg
     4501 acccatccct ctgaatattt taaaaaaggg caggaagttg aagcaatagt attgaatgtg
     4561 gatgtagaaa acgaaaaatt ttccctgggt ataaaacaaa ttgaaaaaaa cccctgggaa
     4621 gatttatccc aaaaatatgc acccggtagt gttg
//
LOCUS       DASVIM010000094         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1655963, whole genome shotgun sequence.
ACCESSION   DASVIM010000094 DASVIM010000000
VERSION     DASVIM010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655963"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VF305_01920"
     CDS             <1..586
                     /locus_tag="VF305_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7415923.1"
                     /translation="IGKENILHSKPQIFVANHQSDFDILIALAYIPVQFRWIAKKELF
                     NIPVFGAAMRSAGYIEIDRQNREKALHSLDEAALRTREGKSVMTFPEGTRSRSGEIKP
                     FKQGPFHLAIKSGVSIVPVSIIGSGQIMPKRSLKISSGKVKLVIDKPIDVKNYTLENR
                     QELIKAVRNVIIKNYDLWREVGTASIKDIESAAT"
     gene            880..>1793
                     /locus_tag="VF305_01925"
     CDS             880..>1793
                     /locus_tag="VF305_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX7415924.1"
                     /translation="MDAKQTDNKGSREIKGVVCLALAVFLLLCLVSYHPQDPSFTRFV
                     AVGKTIHNFTGHVGSYAADSLIRLLGLSSFLLPLIFLFCSFKYFLRPAFFINTSRFLG
                     FFFFALAFAGLLAAILKSGITIYGEALQAGGFIGALIVRLLLNYFNAAGTYIILILVF
                     VITLISMVKFSVVSVTERIFQFALNLITLCKNRLSSFVNFVLGSIEMERKPQSVIEEN
                     SPAAKKAKPKKIEQAHFDFTETIGGSKYQLPPFTLLEEAQHKDARVKRDNLITNSRIL
                     EKKLSDFGVEGRVTEVMPGPVITMYELEP"
BASE COUNT          484 a          382 c          366 g          561 t
ORIGIN      
        1 aatcggcaag gaaaatattc tgcacagcaa accgcaaata ttcgtggcca atcaccaaag
       61 cgatttcgat attttaattg ccttagcata cattcccgtt caattccgct ggattgccaa
      121 aaaagagtta tttaatatcc ccgtatttgg cgcggcgatg agaagtgccg gatatattga
      181 aattgaccgg cagaatcggg aaaaagccct gcacagcctt gatgaagcgg cactgcgcac
      241 ccgcgaagga aaatcagtca tgacttttcc agaaggaaca agaagccgca gcggagaaat
      301 aaaacctttt aagcaaggac cttttcatct cgccattaaa tctggtgtgt ccattgtgcc
      361 tgtttctatt atcggcagcg gccagatcat gcccaaaagg tccctaaaaa taagttccgg
      421 caaagtgaaa ttggttatcg ataaaccaat agacgtgaaa aactatactc ttgaaaatcg
      481 tcaggaactt atcaaggccg tccggaatgt cattattaaa aattatgatt tatggcggga
      541 agtaggcact gcaagcatca aagatataga aagtgcagct acttgaactt ttctaccgct
      601 atagttttgg gtatcgttca aggcttgacc gaactttttc ctgtaagtag ctctgatcat
      661 ttagttatcc tgcaaagtta tttgccggat tttcatcaac ctactgacgg tactgctatc
      721 ccttaaatta ttttttctgg ttatccgcga agcacgattg aaattctttg cttactattg
      781 ctggcttttc ggatctttta ctctgcttgt caaatcgtct tttttctgaa ataatcattg
      841 tcgttgacaa attattttat gtacggcaat ggcaataaaa tggatgcaaa acaaacggat
      901 aataaaggaa gtcgggaaat taaaggcgta gtgtgtctgg ccctggctgt atttctgctt
      961 ttgtgccttg tctcctacca tccacaggat ccttctttta ctcgttttgt tgctgtcggg
     1021 aaaacaattc acaactttac cggccacgtc ggctcctacg cagccgattc tttaatccgc
     1081 cttttgggtt tgagttcctt tcttctgcca ttaatttttc tgttttgctc ttttaaatat
     1141 tttttaagac ctgctttctt tattaatacc agccggtttt tggggttttt cttttttgcc
     1201 cttgcttttg ccggactcct agcggccatt ttaaaaagcg ggatcaccat ttatggagaa
     1261 gcgctgcagg ccggcggctt catcggtgcg ttaatcgtcc ggctgcttct taattatttt
     1321 aatgctgccg gaacttatat cattttaatt ctcgtttttg ttataacgct gatttccatg
     1381 gttaaatttt cagtggtttc tgtgactgaa cgaatttttc agttcgcctt gaatttaatc
     1441 actctttgta aaaatcgcct ctcatccttt gtcaattttg tcttgggcag catagaaatg
     1501 gaaagaaaac cgcaatcggt aatcgaagaa aattcacctg cggcaaaaaa agctaaaccc
     1561 aaaaaaattg aacaagcgca ttttgatttt accgagacaa taggcggcag caaatatcaa
     1621 cttcctccct ttactttatt ggaggaagct cagcataagg atgcccgcgt caagcgggat
     1681 aatctgatca ccaattcgcg tattttggaa aaaaagctat ccgattttgg cgttgagggg
     1741 cgtgttaccg aagtcatgcc cggcccggta atcacgatgt atgagctgga acc
//
LOCUS       DASVIM010000095         1943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_546014, whole genome shotgun sequence.
ACCESSION   DASVIM010000095 DASVIM010000000
VERSION     DASVIM010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546014"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1831)
                     /gene="bamA"
                     /locus_tag="VF305_01930"
     CDS             complement(<1..1831)
                     /gene="bamA"
                     /locus_tag="VF305_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX7415925.1"
                     /translation="MITNKILRFACLTFLFLTLFPLSAAHSQELKKFSVLPFEVYSKG
                     DSAVIKESLYKNLSEELAKEKLIRVISADAFLKGKIKIDKRRAMEIGKSLGADFVIMG
                     SLTKLGETLSIDARIIDVTTANTLPSISAQGKGLVSLGMIAAQLKAEVLIRTGLVQKI
                     AKIEIKGNRKIEASAIIAQIKSKVGNHFFKADITADIKTIFKMGFFLDITAEVISTPE
                     GKVITFIVQEKGLISEIRINGNKALSKDDIQEVLTIKTKQIVDQEKIKADIEKIKVLY
                     DSKGYYNAEITDTVERDGEKDFRVILDIKENDRLYVRTITFEGNEAYSAKELKNMLST
                     SEYGILNFITDSGLLKRDQLRQDINKITAYYLNNGFINAQIGEPEITIDKKGIYIKIQ
                     IKEGKRFKVGKVGISGDLLEKPRVELLSSLKTKEGNNYDRELIMKDMDFLTQACNDEG
                     YANAEVNPQINIREKEQLVDIDFQTKKGELVYISRINITGNTITRDKVIRRQLNIVEG
                     DLYSSSKLKTSYSNLNRLRYFEEVDFQTEKGIDKSKMDINIRVKEKNTGSFMVGAGYS
                     ATDQALIMAQIVQQNFLGYGQTLSLKGGISATSTSIDLSFTEPW"
BASE COUNT          511 a          383 c          332 g          717 t
ORIGIN      
        1 gccaaggttc agtgaaagat aaatctatgc tggtactggt ggcactaata cctcctttga
       61 ggcttaatgt ttgaccatat cccaagaaat tttgttgaac gatttgtgcc atgatcaggg
      121 cctggtcggt ggcgctatag ccggcaccca ccataaagct tcccgtattt ttttctttga
      181 ctcgaatatt aatatccatt ttacttttgt caattccttt ttctgtctgg aaatctactt
      241 cttcaaaata ccgtaggcgg ttcaaattac tgtaactagt ttttaatttg ctgctggagt
      301 ataagtcccc ttcaacgatg ttcaattgac gccggatgac cttatcgcgg gtaatggtat
      361 tgccggtgat gttaatacgg ctaatataaa ccagttcgcc tttttttgtt tgaaaatcta
      421 tatctactaa ttgttctttt tctcgaatat taatttgagg gttaacctcg gcattggcat
      481 aaccttcatc gttgcaagct tgcgttaaaa aatccatgtc cttcatgatt aattcgcggt
      541 cataattgtt accttctttt gttttcagcg atgacaataa ctcgactctg ggtttttcca
      601 gcaaatcgcc ggaaattccc actttgccca ctttaaatct ttttccctct ttaatttgaa
      661 tttttatata aatccctttt ttgtcgatag ttatttctgg ctcgccaatt tgagcgttga
      721 taaaaccatt attgagataa taagcggtta ttttattaat gtcttgtctg agttgatcac
      781 gcttgagcaa tccggaatcg gttataaagt taagaatccc atattctgaa gtgctcagca
      841 tatttttaag ttcttttgct gaataggctt cattaccttc gaatgttatt gttcgaacat
      901 agaggcggtc attttctttg atatccaaaa ttacgcgaaa atctttttct ccgtcccgtt
      961 cgactgtatc tgttatctcg gcattataat aacctttgct atcataaagt acttttattt
     1021 tttctatatc cgctttaatt ttttcctgat caacaatttg cttagtctta attgtcagaa
     1081 cttcttgaat atcatcttta cttaaggctt tatttccgtt aatccgaatt tctgaaatca
     1141 gtcctttttc ctgcacaata aaagtaatga cctttccttc gggtgttgat atcacctctg
     1201 cagttatgtc taagaaaaag cccattttga atatggtttt aatatcagct gttatatctg
     1261 ctttgaaaaa atgattgccg accttacttt taatttgcgc aatgatagcg gaagcttcga
     1321 tctttcggtt acctttgatt tcaattttag ctattttttg aacaagtcca gtgcggatta
     1381 gaacctctgc ttttaattgt gccgctatca taccaaggct aactaaacct ttgccttgag
     1441 cggaaattga aggtaaagta tttgccgtgg tgacatcaat tattcgggca tcaatgctta
     1501 acgtttcacc caatttggtc aagctgccca tgattacaaa gtcagcgcct agagatttgc
     1561 cgatctccat tgcccttcgt ttatcaattt tgattttacc tttaagaaag gcatccgctg
     1621 aaattacccg gataagtttt tcttttgcta attcttcgga caaattttta tatagagatt
     1681 ccttgattac agcgctgtct cctttactgt aaacttcaaa aggcagtaca ctaaattttt
     1741 ttaattcctg agaatgcgct gcggaaaggg gaaaaagcgt caaaaacaaa aaagttaagc
     1801 aggcgaatct aagtatttta tttgtaatca taaatttttc cgtcgcgtag gccgatcctg
     1861 ctcgacatcc tttctgccag caatttgtta tgagttacga caacaagggt aatctttttt
     1921 gtttcattta aattaactag tat
//
LOCUS       DASVIM010000096         3118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1571776, whole genome shotgun sequence.
ACCESSION   DASVIM010000096 DASVIM010000000
VERSION     DASVIM010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571776"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VF305_01935"
     CDS             complement(<1..184)
                     /locus_tag="VF305_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415926.1"
                     /translation="MSSRSSKFLIGLFVITGTLIAAIIIIWVGAADLFMKGSTYVTYF
                     DESVQGLQADSAVKYRG"
     gene            complement(174..968)
                     /locus_tag="VF305_01940"
     CDS             complement(174..968)
                     /locus_tag="VF305_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7415927.1"
                     /translation="MYATENIPIIIVENLTARFGSNIVFEDVSFRVYKGEILVIVGES
                     GCGKSTLLKIMIGLQKPSAGKVLFQGTDITSADEKELNSYRQNIGVLFQSSALFSSMT
                     LKENIALPLQEYTNIDSAMINMIIKMKLGMVNLAGYENHNPAELSGGMKKRAGIARAM
                     ALDPTVLFFDELSAGLDPVTAAELDDLIIKTNEALGTTMVIVTHELESICNIADRVLM
                     LDKAAKGIIAEGRPLELKEKATDPRVRSFFLRKRPVADYQDGFYVK"
     gene            complement(988..2133)
                     /locus_tag="VF305_01945"
     CDS             complement(988..2133)
                     /locus_tag="VF305_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7415928.1"
                     /translation="MDSKQDWPKLYEISYKNEHSHLVIYFKGEFGIKNLKLFTNEINE
                     LVYKQQPQTISIDFSGISYLDSAAALAIIQIKKNAAVKNISCTLVNLNEEAKGIFSVI
                     REQALTQLPYKSKEQQDSFLIQVGEASLDLVRDVINFLTFIGELLTAIFYTIIHPKSL
                     RVKDILFYMKRAGVDGLPIVGLISLLIGLIIAFMSFLQLKQVGANIYVPSLVSFAMVK
                     ELGPIMTAILVAGRSGSAFAAEIGTMVVNEEVDALNTMGFNAIRFLAVPKILATIIVV
                     PILTIYADFFGVIGGMIVGVTGLDLTVYTYVMQSIKTIKVFDVVSSLFKAAVFAGLIA
                     GIGCQRGFQVRAGAQDVGKFTTSAVVSAIFLIVVADSIFAVMFYYLK"
     gene            2354..2548
                     /locus_tag="VF305_01950"
     CDS             2354..2548
                     /locus_tag="VF305_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415929.1"
                     /translation="MSRQSQQKYFEAIHKRYKEAPREEKTIIIDECCATCGYHRKHAI
                     RRLKGVLPRSLFVSLATLAG"
     gene            complement(2580..>3118)
                     /locus_tag="VF305_01955"
     CDS             complement(2580..>3118)
                     /locus_tag="VF305_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX7415930.1"
                     /translation="YNAQKDPIFARFIDTILHQKMSEVCSTTLPQIAIPRIMTHPEYQ
                     NYLIERTRHYEKLSNIAYDILKDVPCLMVNKTNGAFYMTVVFNESALNNHQSLHIKKR
                     EIKEYVQNLVKGSIEHDKRFVYYLLAATGICVVPLTSFFTPLLGFRMTLLDKDEAEFR
                     YVVKTIAEKIVEYIESGK"
BASE COUNT          936 a          660 c          606 g          916 t
ORIGIN      
        1 caccccggta tttaactgcg gaatcggctt gcagtccttg aaccgattca tcaaaatatg
       61 taacgtaggt ggaacctttc atgaataaat cggcagcccc tacccagata atgataatgg
      121 cggcaattag cgtaccggtg atgacaaaga ggccaattaa aaatttggaa cttctacttg
      181 acatagaatc catcctgata atcagcaaca ggcctttttc tgagaaagaa gctgcgtacc
      241 ctggggtctg ttgctttttc ttttaattct agcggcctac cttcagcaat aattccttta
      301 gctgctttat ctaacattaa tactctatcg gcaatattgc aaatagattc taactcatga
      361 gtcacaatta ccatagttgt ccccaatgct tcattagtct taataattag atcatccaat
      421 tcggcggcgg taaccggatc aagcccggcg gatagttcat caaaaaaaag cacggtagga
      481 tcaagcgcca tagcccgggc tatgcccgct cttttcttca tcccgccaga aagctccgcc
      541 ggattatggt tttcatagcc agctagattg accatgccca atttcatttt gataatcata
      601 tttatcatag ctgaatcaat attagtgtat tcttgcaggg gtaaggcaat attttctttc
      661 aatgtcatcg agctgaacaa agcgctggat tgaaaaagca ctccgatatt ttgccggtag
      721 ctgtttagtt ctttttcatc cgccgaagtg atatcggttc cttgaaacaa aactttccct
      781 gcggacggct tttgtagccc aatcattatc tttaaaagag ttgatttgcc acaaccactt
      841 tcgcctacaa taaccaatat ttcccctttg tatacccgaa agctaacgtc ttcaaacaca
      901 atgttgctgc caaagcgggc ggtaaggttt tccacgataa ttatcggtat gttttccgtt
      961 gcgtacaaaa ctcactcctt aaagttttta cttaagataa taaaacatca ctgcaaaaat
     1021 cgaatctgcc actacaataa gaaaaattgc ggagacaacg gcagaggttg taaattttcc
     1081 gacatcctgc gcaccggcgc gcacttgaaa tcctctttgg caaccgatcc cggcaataag
     1141 accggcaaag acagcggcct taaataggct cgagactaca tcaaatactt taatggtttt
     1201 tattgattgc ataacatagg tgtacacagt gaggtcaagg ccggttacac caacaatcat
     1261 gccgccaata acgccgaaaa aatcagcata gattgtcaat atcggaacaa ctattattgt
     1321 cgccagtatt ttgggcacag ccagaaatct aatggcgtta aaacccatcg tatttaaagc
     1381 atcaacttct tcattgacca ccatggtgcc gatttctgcg gcaaaggctg aaccggagcg
     1441 gccggccact agtatagccg tcataatcgg cccaagttcc ttgaccatcg caaagctaac
     1501 caaagaagga acataaatat tggctccgac ttgctttagc tgtaaaaaag acataaaggc
     1561 aatgatcagc ccgatcaata aactgattag cccgacaatc ggcagaccat caactccggc
     1621 gcgtttcatg taaaaaagta tatctttgac tctcaatgat ttcggatgaa taatagtata
     1681 aaatatagca gttaacagct caccgataaa cgttaaaaag ttgataacat cgcggacgag
     1741 atctaaagag gcttctccca cttggatcaa aaagctgtct tgttgttctt tagatttata
     1801 tggcaattga gtcagcgcct gttcacgaat tacactgaaa attccttttg cctcctcatt
     1861 taaattaact agagtacaag aaatgttttt tacagccgca ttctttttga tttgaattat
     1921 cgctaaagct gccgcactat ctaaatagga tatgccggaa aagtctatac tgattgtttg
     1981 cggctgctgt ttataaacca actcatttat ttcattggta aataatttta agttttttat
     2041 cccaaattct cccttgaaat aaataacaag gtgtgagtgc tcgtttttgt aagatatttc
     2101 gtagagtttt ggccaatcct gtttgctatc cataatcact tattgttgtt ctcggccgga
     2161 aagatatatt ctcaaatcaa atatttaaaa tatttttaaa tagttaacgc ttttgccatg
     2221 attttgtcaa tgcccaatta atagactgct aattgcctcc aataaaatgt taccgaagat
     2281 gtcggtaaaa aatggacgtt gactcataaa gcgatgttac cgaagaaaga ttcggaaacg
     2341 gagatataag tatatgagtc gacagtccca acaaaaatat tttgaggcca ttcataagcg
     2401 ctataaagaa gcgccccgtg aagaaaagac tattatcatc gatgaatgtt gtgccacctg
     2461 tggttatcac cggaagcatg cgatccgtcg cttaaaagga gttttgccaa ggtctctatt
     2521 tgttagtctc gctacgctag cgggatagcg aagcgtatgg ggatggagtt taataccatt
     2581 tattttcccg actcaatata ttccacgatt ttttcggcga tggttttgac tacgtatctg
     2641 aattccgctt cgtccttgtc taatagtgtc atgcggaaac caagtagcgg agtaaagaat
     2701 gatgtcagcg gcacgacgca aataccggtt gccgccaaaa gataataaac gaatcttttg
     2761 tcgtgttcga tggagccttt gactaaattt tgcacgtact ccttgatttc ccgcttctta
     2821 atatgaaggc tctggtgatt attaagtgca gactcattaa aaactacggt catgtaaaat
     2881 gcgccgttgg tcttgttcac cattaaacag ggaacatcct ttaaaatatc gtaagcaatg
     2941 ttagaaagtt tctcgtaatg acgcgtgcgt tcaattaaat aattttgata ttcagggtga
     3001 gtcataatcc gcggaatagc gatttggggt agcgtcgtcg aacacacttc cgacattttt
     3061 tggtgtaata tagtatcgat gaaacgcgcg aaaatcggat ctttttgggc attataaa
//
LOCUS       DASVIM010000097         3127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_121284, whole genome shotgun sequence.
ACCESSION   DASVIM010000097 DASVIM010000000
VERSION     DASVIM010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121284"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..978
                     /locus_tag="VF305_01960"
     CDS             337..978
                     /locus_tag="VF305_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEX7415931.1"
                     /translation="MKYYPVFWDIRGKPCVVVGGGEVAARKVKRLLDCGAKVSVVSPE
                     LTLELAALKDADMISHIAREYLSEFIYGSALVIGATDDEKTNSMISHDAREKGIPVNI
                     VDDPQKCDFILPSIVERGDLVIAIGTGGNSPALARHLREELESRYGAEYEIFLQILGR
                     LRSQMTKNAGAGRSWFDSLISSGILELIRKKDWEKVERTVREITGQEVKIDIV"
     gene            965..1795
                     /gene="ccsA"
                     /locus_tag="VF305_01965"
     CDS             965..1795
                     /gene="ccsA"
                     /locus_tag="VF305_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX7415932.1"
                     /translation="MTLFSLEMILFKLALAFCTLCTFGYLLSLLIKRVMLAKVSTWIL
                     TIAFGFLTFNLLTAFINSAGHVNPGSRAFFLFCAWAVAGLYLVFQFKTKTRLLGDFIS
                     PFILLLMIAAAGQETGKSLLPENLQSWLTTLHFVFAIIGEALFVLASCAGAMFIIQNG
                     LLKHKKLGKMSRLLPSLSDLDRINYLCLLWGFPILTIGIIAGAVFAGMAWKPLWPTDP
                     KIIWSLAAWVIYGFLLHQRLAIGWKGSRMAVLSCAVFILFLLSFLAVRFCFTTWHGFI
                     "
     gene            1799..3061
                     /gene="hemA"
                     /locus_tag="VF305_01970"
     CDS             1799..3061
                     /gene="hemA"
                     /locus_tag="VF305_01970"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX7415933.1"
                     /translation="MILLIGLNHKTAPLAVREKIFAGCEEKNNLLSDLLGLSGVQETL
                     YISTCNRVELVCVMDGEEETIHGPKRFLSQSGNLTDQEAAACLYVYRDAEAIRHLFRV
                     AASLDSLVMGETQILGQVKEAYRQALAQNATGLVLNRLMHRSFRTAKRVRTETAIAAN
                     PVSVSFAAVELAKKIFGSLTGKKILLIGAGEMAELTGTHLIGNGAEEITVANRSQAQG
                     TALAERFHGKAILLDDLDAALIDADIVISSTGAASFVVTEELIRHSMHKRKNRLLFLI
                     DIAVPRDIEPTADSIENVYLYNIDNLQDIVDENMKVRKKEALKAEAIVEEEVLHYNNW
                     IKELEAVPTIVSLRAKAEGIVRKELEKSQGFLQKLSEEDRGKVDNLVKAVVNKILHAP
                     VTVMKEESSEVSSQEIVAAVRQLFRLDE"
BASE COUNT          806 a          646 c          806 g          869 t
ORIGIN      
        1 aaagtaaata ttttgatctc tcaaaaaaaa ccaaggagaa atattgatca tattttttga
       61 tttgtgaata ttgcccatag tttgtcattc cgggctcaga gccgggtgaa taacctaaag
      121 gtcacacgta acctaatacc ctaaaaggca cgcgaggtca tacgtaaccc gataccctaa
      181 taacctgaag gttacacgag gtcacgcgtg cgagccgggg atgagaaaag cgccttttta
      241 atgacgaatc cccgggcttg aaatttaccc ctcttcgtga tagtttccat gactcggttt
      301 cggcaacgta actatgccgc ggtagaaagg taaccattga aatattatcc tgttttttgg
      361 gacattcggg gaaaaccttg tgtcgtcgtg ggcggtggtg aagttgccgc aagaaaggtg
      421 aaaaggctac ttgattgcgg cgctaaagta tctgtcgtca gcccggagct aactttggaa
      481 ctggccgctc taaaagatgc tgatatgatc agtcatatag cacgcgaata tttaagtgag
      541 ttcatttatg gatcggcatt ggttattgga gcaacagatg atgaaaaaac gaattcgatg
      601 atatcccacg atgcccgcga gaagggcatt cccgtaaata ttgttgatga tccgcaaaaa
      661 tgtgatttca ttttgccctc gattgtggaa agaggcgatc tggtaattgc tatcggcacc
      721 ggtggcaact ctcctgcgct tgcccgccat ttgcgcgaag aattagagtc tcgctacggc
      781 gctgagtatg aaatatttct ccagattctg gggcgtctgc gttcccaaat gacaaaaaat
      841 gccggtgccg gtagaagctg gtttgattcc ttgatctctt caggaatatt ggaattaatc
      901 cgaaaaaaag attgggaaaa agttgaaagg actgtacggg agataaccgg tcaggaggtg
      961 aaaattgaca ttgtttagtt tagagatgat tttattcaag ctggccctgg ctttttgcac
     1021 tctttgcact tttggctatt tactttctct tttgattaag agagtaatgc tggccaaggt
     1081 ttctacctgg atcttgacca ttgcctttgg cttcttgaca tttaatttac ttactgcctt
     1141 tataaattcg gcaggacatg taaatccggg ttcacgcgct ttctttttgt tttgtgcatg
     1201 ggcggttgcc gggctttatc tggtgttcca atttaaaaca aagacaagat tattgggtga
     1261 tttcatttcc ccgttcattt tattactgat gattgcagct gccggacagg aaacagggaa
     1321 atctttactg ccagagaatt tgcaaagttg gctcaccaca ttgcattttg tttttgctat
     1381 tatcggggaa gcgctttttg ttctggcctc ttgcgccggc gccatgttca tcatccaaaa
     1441 cggtttgctc aagcacaaga agctaggcaa gatgagccgg cttttgccat cgcttagtga
     1501 tttagaccgg attaattatc tttgcctgct gtggggcttc cccattttga caataggcat
     1561 tattgcggga gccgtttttg cgggcatggc ctggaagcct ctctggccta cggacccgaa
     1621 aattatctgg tctttggcgg cctgggttat ttatggattt ctactgcatc agcgtctggc
     1681 cattggctgg aaaggttccc ggatggcggt attatcttgc gcggtgttta tccttttcct
     1741 gttgtccttt ttggctgtga gattttgttt tactacttgg catggtttta tttagaaaat
     1801 gatattatta atcgggttaa atcataaaac ggcgccgctc gctgttcgcg aaaaaatttt
     1861 tgccggttgc gaggagaaaa ataatttatt gtctgatctt ctcgggttgt caggtgtgca
     1921 agagactctc tatatttcca cctgtaaccg ggtcgaactg gtttgtgtga tggacggcga
     1981 ggaggagaca attcatggtc cgaagcgatt tctttcccaa agcggtaatc ttaccgacca
     2041 ggaagctgcc gcttgcctct atgtctatcg tgacgcagaa gcaatacggc atctgtttcg
     2101 ggtggcggca agtctcgatt cgctggtcat gggtgaaaca cagatattag gacaggtcaa
     2161 agaggcttat cggcaggcgc tggcgcaaaa cgcgaccggc cttgtgctca atcgtctgat
     2221 gcaccggtca ttccggacgg ccaaacgggt acgcacggag acggcgatag cggcaaatcc
     2281 agtatcggtc agttttgccg cggtggagct ggctaaaaag atattcggct cgcttaccgg
     2341 caagaaaata ttgctgattg gagcggggga aatggccgaa ctgaccggca cgcacttgat
     2401 cggtaacggc gctgaagaaa taaccgttgc caacagatct caagctcaag ggacagctct
     2461 tgccgaaagg tttcacggga aggcaatttt gcttgatgat cttgacgcag cgttgattga
     2521 tgcagatatt gtgatcagtt ccaccggcgc ggccagcttc gttgtcacgg aagagttaat
     2581 cagacacagt atgcataaac ggaaaaaccg tctgttgttc ttgattgata ttgccgttcc
     2641 ccgtgatatt gaaccgacgg cggatagtat tgaaaatgtc tatctttaca acatcgataa
     2701 cctgcaggac attgtcgatg agaacatgaa ggtccgcaaa aaagaggcgc tcaaggctga
     2761 agcaattgtg gaagaagaag ttctccacta taataattgg attaaggaac tggaagctgt
     2821 tccgaccata gtatcgctgc gcgcaaaggc cgaaggcatt gtgcgaaagg agttggaaaa
     2881 atcacaaggc ttcctgcaga agctaagtga agaagacagg ggaaaagttg acaatctggt
     2941 aaaagcagta gtcaacaaaa tactgcatgc tccggttacg gtgatgaaag aggagagttc
     3001 cgaagtaagt tctcaagaaa ttgttgccgc ggtacggcag cttttccggc tggatgaata
     3061 aatgtagatg cggcagttga agggacatcc catcgctcag tattccctgc aactgtggct
     3121 ctaaagc
//
LOCUS       DASVIM010000098         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_633410, whole genome shotgun sequence.
ACCESSION   DASVIM010000098 DASVIM010000000
VERSION     DASVIM010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633410"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /gene="ispH"
                     /locus_tag="VF305_01975"
     CDS             complement(<1..1710)
                     /gene="ispH"
                     /locus_tag="VF305_01975"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX7415934.1"
                     /translation="MEIKLAKTAGFCMGVRRAVDIVLEIAQHETGRRIYTYGPLIHNP
                     QTIELLKSRGINAINNIDEINERQKAVLIIRAHGIAPAERKKLKESGIKIIDATCPKV
                     GYVQAIIKKHTALDYTVVIVGDKEHPEVDGLLGYTGGRGITLATLDDVAKVPPLSKVC
                     IVAQTTQDSDKYNKIVKKIQELYPQAIVFNTICSSTQQRQAEVIAMAGEMDAMFIVGG
                     KNSANTRRLADLSQKQQTPTFHVEAAAEMKNINLGPYNRIGVSAGTSTPNWIIDRVMD
                     RIAEGQSSKLKKVGWLLNFWIWAIKTDVYSALGAGCLCLACMLLQEIPLNISSIAIAS
                     FFVYAMHVLNRLISRKPLGLIGSFREETYLQHEKSYLYAAVASIALALVLAFINGALP
                     FTFLFIISLAGVLYNMKIMPKGWRFQSLKDLPGSKNIFMATAWGMVAAILPALNNYYS
                     FNGGLIIAFAFTFGVVFVRSAMSDILEIQSDKLIGRETIPVLFGKERTVIVLKIISLM
                     LFVFLLFSYPAGWSSSLSFFLLPCILYIWICLQLCDKKSGFSGAVMEGLLETSYIIAG
                     FCVL"
     gene            complement(1700..2449)
                     /locus_tag="VF305_01980"
     CDS             complement(1700..2449)
                     /locus_tag="VF305_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX7415935.1"
                     /translation="MEKVRLDKLLVASGLAPTIKKAQALILAKAVKVNEIFLDKADTL
                     VPADAEIHIKGADNPYVSRGGLKLKGALEALKLNVEGMVALDVGASTGGFTDCLLQAG
                     ARKVYALDVSYGQLAWRLREDKRVVVIERTNIRYYNGIDLDEKPALATIDASFISLKI
                     IFPAVLRLLQDEAQILALIKPQFEAARGEIGKNGVVKDPAIHDRVVQEISGFCQSLNL
                     EVIGTCASSLLGPAGNREFFILVRKKKLNGN"
BASE COUNT          716 a          586 c          488 g          669 t
ORIGIN      
        1 aagaacacaa aaacctgcaa taatatagct agtttccaga aggccttcca taactgcccc
       61 ggaaaacccc gactttttat cacaaagctg taaacaaatc catatatata atatacaggg
      121 caacaagaaa aagctcaaag atgaagacca acctgccgga taagaaaaaa gaagaaaaac
      181 aaataacatc aatgagataa ttttgaggac gattactgtg cgctctttgc caaatagcac
      241 gggaatcgtt tcccgtccga ttaatttatc actttgaatt tctaaaatat ccgacatcgc
      301 tgagcgaaca aaaacaaccc cgaaagtaaa agcaaaagca atgatcaatc cgccattaaa
      361 ggaataataa ttattcaagg cgggcaaaat ggccgcgacc atcccccagg cagtagccat
      421 aaatatattt ttcgaaccgg gtaaatcttt taaactttgg aagcgccaac ctttgggcat
      481 aattttcata ttgtaaagaa caccggccaa cgaaataata aacaaaaacg taaaggggag
      541 agcaccatta ataaacgcca acacaagagc aagcgcaata gaagccacgg cggcatagag
      601 ataacttttt tcatgctgaa gatatgtttc ctcccggaaa gaaccaatta atcctaacgg
      661 tttacggcta atcagccgat tcaatacatg catcgcgtag acaaaaaacg aagcaatcgc
      721 aatagaagat atattcagcg gtatctcctg cagcagcatg caggccagac aaaggcagcc
      781 agcacccaga gccgaataaa catcagtttt tatcgcccat atccaaaaat tgagcagcca
      841 gccaactttt tttaatttac tgctttgacc ttccgctatc ctgtccatca cccggtctat
      901 aatccagttc ggagtagagg tgcccgccga aaccccgatg cgattgtagg ggcctaaatt
      961 gatatttttc atttctgcag cagcttcgac gtggaaagtt ggtgtttgct gtttttgtga
     1021 aagatcggcc agccttcgag tattggcgct atttttgccg ccgacaataa acatagcatc
     1081 catttccccc gccattgcta taacttccgc ctgcctttgt tgcgttgaag agcaaatagt
     1141 attaaataca atagcctggg gatagagctc ttgaattttt ttaacaatct tgttgtattt
     1201 atccgaatct tgcgttgtct gggcaacaat gcaaactttg ctcaggggag gcactttagc
     1261 tacatcatct aatgtagcta aagtaatgcc ccgaccgccg gtgtaaccca gaagaccgtc
     1321 aacttccgga tgttccttat cgcctacaat cactaccgta taatcaagag cagtatgttt
     1381 tttgatgatg gcttgcacat aaccaacttt ggggcatgtg gcatcaatga tttttatgcc
     1441 actttcttta agtttttttc tttcggcggg ggcaatgccg tgtgcacgaa taatcaggac
     1501 agctttttgc cgttcgttta tttcatcaat gttgttgatt gcattaatcc cgcggctttt
     1561 gagtagttca atggtttgtg gattgtgaat taatgggccg tatgtataaa tgcggcggcc
     1621 ggtttcatgt tgcgctattt ccagcacaat atccaccgcc ctgcgcacac ccatgcaaaa
     1681 tcccgcagtc ttggcgagtt taatttccat ttagcttttt ctttcgcact aaaataaaaa
     1741 attctctgtt acctgccggg cctaaaagag atgaagcaca agttcctatg acttctaaat
     1801 taagagattg acaaaatccg ctaatttctt gcactacacg atcatgaata gcgggatctt
     1861 tgactacacc gttcttgcct atctctcctc ttgccgcctc aaactgcggt ttgattaacg
     1921 ccaaaatttg cgcctcatct tggagcaagc gcagtaccgc aggaaagata attttcagcg
     1981 aaatgaaaga ggcatcaatt gtggctaagg cgggcttttc gtccagatcg atgccattat
     2041 agtagcggat attggttcgt tcgatcacga ccacgcgctt gtcttcgcgt agcctccagg
     2101 ccagttgacc atagctaaca tccagagcgt aaactttccg cgcacctgcc tgtaaaaggc
     2161 aatcggtaaa gcctccggta gaagccccca catcaagggc aaccatacct tcaacattca
     2221 attttaacgc ttccagagct ccttttaatt tcaggccgcc gcggctgacg taaggattat
     2281 ccgcaccttt tatgtgtatc tcagcgtcgg ctggaacaag agtatccgct ttatccagga
     2341 agatttcatt aactttcacc gccttggcta aaatcagcgc ctgtgctttt ttaattgtcg
     2401 gtgccagacc gctcgctacg agcaatttat ctaagcgaac tttttccatt tactcttta
//
LOCUS       DASVIM010000099         3448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1145333, whole genome shotgun sequence.
ACCESSION   DASVIM010000099 DASVIM010000000
VERSION     DASVIM010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145333"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VF305_01985"
     CDS             complement(<1..933)
                     /locus_tag="VF305_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX7415936.1"
                     /translation="MEQVKVLLQDREIPKKWYNIVADLPTPMKPPLHPGTGQPVTPGD
                     LAAIFPMNIIEQEASGKRWIKIPEEVLQKYLLWRPSPLYRAYNFEKMLNCPVKIYYKH
                     EGVSPAGSHKPNAAIPMAYYNKMAGTKRMVTETGAGQWGSALAMATQMFGLECRVYMV
                     KISYEQKPYRRILMNTWGAECISSPSNLTQAGRNVLAEHPDSPGSLGIAISEAIEDAV
                     NGKNARYGLGSVLNHVLLYQTIIGLEAQKQMKKIGVYPDVVMGCCGGGSNFAGISLPF
                     VCDKIHGKKVKIVGAEPSSCPSMTKGPVDYDFGDL"
     gene            complement(1241..2188)
                     /locus_tag="VF305_01990"
     CDS             complement(1241..2188)
                     /locus_tag="VF305_01990"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HEX7415937.1"
                     /translation="MEKIKLGVSSCLLGENVRYDGGHKLDRFLTDTWGEYVEYIPVCP
                     EAECGLGVPREAMHLEGDPAFPRLITTSTRQDMTRRMMTWAHKRVAQLEKEALCGFIF
                     KSDSPSSGRERIKVYNEKGMPVKKGVGIFARIFMEHFPLLPAEDDGRLHDPGLRENFI
                     ERIFALRRWRDILGKKESNGNLVDFHTRHKLLILAHSPKHYQIMGKLVGGQKSIPGQD
                     LYEQYQTLLLESLELKTTLKKNANVLQHMMGYFRQQLSVDEKEELMEVIEYYREGYIP
                     LIVPVTLISHYIRQYDQPYLREQVYLNPHPLELQLRNHV"
     gene            2767..3432
                     /locus_tag="VF305_01995"
     CDS             2767..3432
                     /locus_tag="VF305_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEX7415938.1"
                     /translation="MAKSDAALEEKKLLFPNGAIKESKFLQKGKLEGEYTSYFNNGQI
                     IARLHFVRGKKNGAAESYYNNGQVREKTNFNNDKMDGPYISYYENGHIREQESYKDGK
                     LDGKYQIYYKTGQLKEEENFKNGKFDGEYICYYKSGQIKEKGYFINDKHEDEYKAYFK
                     NGQIKEQATYRNGNLVGKFLTVNENLSDAPETSADSSADIWEEKDVANLLQDLTNFDK
                     KQK"
BASE COUNT         1047 a          715 c          673 g         1013 t
ORIGIN      
        1 caagtcgcca aagtcatagt caacaggccc tttggtcata ctggggcaag aagatggttc
       61 ggcgcccact atctttactt ttttcccgtg aatcttatca cagacaaagg gcagggaaat
      121 tccggcaaag ttgctgccgc cgccgcaaca tcccatgact acatcgggat aaacaccgat
      181 ttttttcatt tgtttttgcg cttccaagcc gattattgtc tgatagagca ggacatgatt
      241 caagacgctg cccaatccgt aacgtgcatt tttaccattc acggcatctt caattgcttc
      301 gctgatggca atacccaggc ttccgggaga atcaggatgc tcggctaaaa catttctacc
      361 cgcctgcgtt aagttgctgg gactggaaat acattcggct ccccaggtat tcataagtat
      421 gcgtcgataa ggcttttgtt catagcttat tttcaccata taaacgcggc attccaaacc
      481 aaacatttgg gtagccatgg ccaatgcgct gccccactgt ccggcgccgg tttccgtaac
      541 cattcttttt gttccggcca tcttattata ataagccata ggaatagcgg cattgggttt
      601 gtgtgagcct gcagggctga caccttcatg tttatagtaa attttaacgg ggcagttaag
      661 cattttttca aaattgtaag cgcggtaaag aggcgatggg cgccagagta aatacttttg
      721 caaaacttct tccggaattt ttatccaacg cttaccagaa gcttcttgct caataatgtt
      781 catcgggaaa attgccgcca aatcaccggg agttaccggt tgaccggtgc cggggtgcaa
      841 aggcggcttc atcggtgtag gcaaatccgc tacgatgtta taccactttt tgggtatttc
      901 acgatcttgc agtagtactt ttacctgttc cataattatt ctcctttcat tgggtttctt
      961 tttacaaacc taaaaaaaat gccatggaat atgtttcttc ccatggcatt gattttagtt
     1021 tccagagtca tgggaaggag cttccctgcc catgctcatt tttccgttcg agcttatggg
     1081 cagacatcta acatctgcca ccaccagtta tttttgaaaa taattactaa cattctcata
     1141 aacgatattt tttttgaatt taagaaagcc gtaacacagc gtataaatag ttgtcaagat
     1201 gaaatttagc atgatcgata acgagattat tttaattatt tcaaacatga ttgcgaagtt
     1261 gaagctctaa cgggtgcgga ttcaggtaaa cttgttccct aaggtacggt tgatcgtact
     1321 ggcgaatgta atgactgata agagtaacag gcacaatcaa aggaatatag ccttcccgat
     1381 aatactcgat tacttccatt agttcctcct tttcatcgac ggataattgt tgcctgaagt
     1441 atcccatcat gtgctggaga acattggcat tttttttcag cgtcgttttc agctcaaggg
     1501 attccagaag cagagtttga tattgctcat agagatcttg cccaggaatg cttttctgtc
     1561 cgcccacaag ctttcccatt atctgataat gtttggggct gtgggcaagg atcaacaatt
     1621 tatgccttgt atgaaaatcc accaagtttc cattactttc tttcttccct aaaatgtctc
     1681 gccatcgtct taacgcaaaa attctttcga tgaaattctc ccgcaggcct ggatcatgta
     1741 agcgaccatc atcttccgct ggcagcaaag gaaaatgttc cataaaaatt cgggcaaata
     1801 ttcctactcc ttttttaacg ggcatccctt tttcattata gacttttatc ctttcccttc
     1861 cactgctggg agaatcgctt ttaaaaataa agccgcaaag agcttctttc tccagctgag
     1921 caacgcgttt atgcgcccaa gtcatcatgc gtcgggtcat atcctgtctg gtactggtcg
     1981 tgattagacg gggaaaagcg ggatcgcctt ccagatgcat agcctctcgt ggaacaccga
     2041 gaccacattc agcttcaggg cagacaggaa tatattctac atattctccc caagtatccg
     2101 taagaaaccg atccagctta tgcccgccat cataacggac attttcaccc aacagacacg
     2161 agctaacgcc tagcttaatc ttttccaatg aagtctccat tgaatactaa atttacagta
     2221 gtcgttccgg gcgaagcgaa aaatcttttt gcttcgctat ttgatttcta tagaaaaccg
     2281 attataaaac aaataaaaat aataacatag gcgaccattt ttaatgctca ccaattaacc
     2341 tgaagcgctt ttcaattacc tgcaaaaaat taacaagtta ttgaaggggg taaaccgcct
     2401 atttattatt aatgaatatg gctgggcatt cctggtcgaa aaatctttat cgcggcggtg
     2461 ccaataatca gggaaataaa gtatccggct gtcaaactct aagagctttt tccccaaaaa
     2521 tattaaatta aatttatcaa caattcagtt ctttttagtt gcattatcat aaatgattgt
     2581 ttaaaaaaag caagggtttg atcatataaa agcaggaggg atatttttat gctgaaaaaa
     2641 gctcttttat attcattatg gaagaaaaaa ccaggcctct ccaatgggac accatgctga
     2701 ccattggatt tggattctta attcattttt taaaaacttt aactttataa ctggaattat
     2761 gtttttatgg ccaaatccga cgctgctcta gaggaaaaaa agttgctttt tcccaatgga
     2821 gctattaaag aaagcaaatt tctccaaaaa ggtaaattag aaggtgaata tacttcttat
     2881 ttcaataatg gtcagataat agcgcgtctg cattttgtcc gtggcaagaa aaacggggca
     2941 gcagaaagtt attataacaa cggccaagta agggaaaaaa caaattttaa taatgacaaa
     3001 atggatggtc cctacatttc ttattacgaa aacgggcaca tcagagagca agagagttac
     3061 aaggatggca agctcgacgg caaatatcaa atttattaca aaaccggcca attgaaggaa
     3121 gaagaaaatt tcaagaatgg caaattcgac ggcgagtata tttgctacta taaaagtgga
     3181 caaattaagg agaagggtta ttttataaac gacaaacacg aggacgaata caaagcttat
     3241 tttaaaaacg gacagataaa agaacaggct acttacagaa atggaaatct tgtgggcaaa
     3301 tttcttactg tcaatgaaaa tttatctgat gcaccggaaa caagcgctga cagttcagca
     3361 gatatttggg aagaaaagga tgtcgctaat ttacttcaag acttaaccaa ttttgacaaa
     3421 aaacagaagt aataatctat ataatttt
//
LOCUS       DASVIM010000100         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_805186, whole genome shotgun sequence.
ACCESSION   DASVIM010000100 DASVIM010000000
VERSION     DASVIM010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805186"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /gene="argS"
                     /locus_tag="VF305_02000"
     CDS             <1..1014
                     /gene="argS"
                     /locus_tag="VF305_02000"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX7415939.1"
                     /translation="KYLTADKEDTVKYFTTIAGERILAEIKDDLQDFGVTFDEYFSEK
                     ELYRNNGVTELLDKLQKKGFVYFDGETLWFKTTDFGDEKDRVVVRKTGEPTYFAADIA
                     YHQNKFARGFEMIIDIWGADHHGYMPRLWAGIQALGHDKNELKIILVQLVNLLRDGVP
                     VAMSTRSGEFVTLREVLEEVGKDAARYNFLMRRSDSHLDFDLEIAKKQSNENPVYYVQ
                     YAHARICSILRMAQERGIALPAYADIDPALLNEPEEKTLIKLMVRYPETIYGAAKTLE
                     PHRITFYLNELAGIFHSYYNKNKVISENVQLTAARLFLVKAISIVIQNTLKILGVDAP
                     EKM"
     gene            1234..>1798
                     /locus_tag="VF305_02005"
     CDS             1234..>1798
                     /locus_tag="VF305_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415940.1"
                     /translation="MASRNIKNFEMKMGKTGLIIVIVGMAALLCTVFLLGVDIGKNID
                     TYPEQIASIPQKVIARIWRPAKLKADQDVPDNKSGQNQPPAKENIDLTFYNTLTSKKG
                     VVKEQPVPDRQKVVETPAEKPVETKPQNEEIGTGDLNVEAQKPSVPVSEKTNEEGKPK
                     AKEAAASIAFHKQKFIIQAASLKEKNKA"
BASE COUNT          549 a          360 c          423 g          466 t
ORIGIN      
        1 aaatatttaa ccgccgataa agaagatacg gtcaaatatt ttaccacaat tgccggagag
       61 agaatcctcg cggaaattaa agatgattta caagacttcg gcgttacttt cgacgaatat
      121 ttcagcgaaa aagaattata cagaaataac ggtgtgactg agcttttaga taaactgcaa
      181 aagaagggat ttgtttattt tgatggcgaa acactctggt ttaaaacaac tgattttggc
      241 gatgaaaaag accgtgttgt tgtccgcaaa accggcgagc cgacttattt tgccgccgat
      301 attgcgtatc atcaaaataa atttgcccgc ggcttcgaaa tgattattga catctggggc
      361 gcggaccatc atggctatat gccgcggctt tgggcgggga ttcaagctct cggtcatgat
      421 aaaaacgagc tgaaaataat tctggtgcaa ttggtaaatt tattgcggga cggtgttcct
      481 gtggctatgt ccacccgttc cggtgaattt gtgactctcc gggaagtcct cgaggaagtc
      541 ggcaaagatg ctgcccgcta caattttctc atgcggcgtt ccgatagcca cctggatttt
      601 gatctggaaa ttgctaaaaa gcaatcaaat gagaatccgg tttattatgt ccaatacgcc
      661 catgcgcgta tttgcagcat actgaggatg gcgcaggagc ggggtattgc cctgcctgct
      721 tatgccgata ttgatcctgc ccttttaaac gagccggaag aaaaaacatt gatcaaatta
      781 atggtgcgtt atccggaaac catttacggc gccgccaaaa ctctggagcc gcatcgtatt
      841 acgttctatc tgaatgaact ggccggaatt tttcacagct actataataa aaacaaggta
      901 atttcggaaa atgttcaatt aacggcggcg cggctattct tggtgaaggc aatcagcatt
      961 gttatccaaa atactttgaa aattttaggt gttgatgcac cggaaaagat gtaaagaatg
     1021 acttcggaaa aagtcccagc atacgccggg tcgcgcatat gcggagtgta tccggcgtat
     1081 gccggatgtg cggcatcctt tgtcattgca gcgtatataa aatacgctgc attcctcagg
     1141 attggcacgc cttgcatctg gagctcgcgt gccctcgtgt cacctttagg tgattagggt
     1201 attttgcaaa gtcttaataa aaggtaagca attatggctt ccagaaacat taaaaacttt
     1261 gaaatgaaga tggggaagac gggattaatc atcgttattg ttggaatggc cgctcttttg
     1321 tgtacggttt ttttgttggg cgttgatatc ggaaaaaata ttgatactta tcccgagcaa
     1381 atagcttcaa ttcctcaaaa agtaatagcg cgtatctggc gtccggctaa acttaaagct
     1441 gaccaggatg tccctgataa caaaagcggg caaaaccaac ctccagccaa agaaaatatt
     1501 gatctgacat tttacaatac tttgaccagt aaaaaaggcg tggttaaaga acagccggtt
     1561 ccggacagac aaaaagttgt ggagactccg gcggaaaaac ccgtagagac gaaacctcaa
     1621 aatgaagaaa ttggtaccgg cgatcttaac gtggaagctc aaaagccgtc agtgccggta
     1681 agtgagaaaa ctaatgagga aggcaaacct aaagcaaaag aagctgcagc gtctattgct
     1741 ttccataaac aaaaattcat aattcaagcc gcttctttga aggagaaaaa taaagcat
//
LOCUS       DASVIM010000101         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1573663, whole genome shotgun sequence.
ACCESSION   DASVIM010000101 DASVIM010000000
VERSION     DASVIM010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573663"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VF305_02010"
     CDS             complement(<1..927)
                     /locus_tag="VF305_02010"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX7415941.1"
                     /translation="MRILLAGARIIDPAQKIDADIDILLEDGKIAKIGADLLKPAKSK
                     DSGKVKIIKLDGMILTPGLIDMHTHLREPGFEYKETVASGVAAAVTGGFTSIACMPNT
                     SPVNDNRSITEFIRRKAVEAALANVYPIGAISKDSSGSQLTEYWDLKDAGVVALSDDG
                     KPVMDAALMRRALEYAYSLGLPVISHCEDINLSGGGLMNEGYYSTILGLRGIPSIAEE
                     AMIARDILIAEFTKTCVHIAHVSTTGSVRLIREAKKRGLKVTAETAPHYFTLTDEALQ
                     DYDTNLKVNPPLRSVQDVKAIKEGLADGTLDVI"
     gene            complement(924..1850)
                     /locus_tag="VF305_02015"
     CDS             complement(924..1850)
                     /locus_tag="VF305_02015"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX7415942.1"
                     /translation="MKLEKKDILGMKDLRVDEINLILETAESFLEISTREIKKVPTLR
                     GKTIINLFYEASTRTRTSFEIAGKRLSADTVNISASTSSVVKGETLIDTARNLEAMNP
                     DVIVIRHSAAGAPHLLASLVKQSIINAGDGAHEHPTQALLDMMTIKEKKGKIAGLKVA
                     IVGDIAHSRVARSNIYGLSKMGAQVFLAGPATMLPRDIEQMGVKIFNKLEDAIVEADV
                     VMMLRIQLERQKQSIFPTLREYSQHYCLNRNNIKLAKKDVIVMHPGPINRGVEISPDI
                     ADDPSYSVILDQVNKGVAVRMALLYLLTGGNE"
     gene            complement(1874..2422)
                     /gene="pyrR"
                     /locus_tag="VF305_02020"
     CDS             complement(1874..2422)
                     /gene="pyrR"
                     /locus_tag="VF305_02020"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX7415943.1"
                     /translation="MAENKNTKIVMDKEGIDRCLTRIAYEILEKNKGVENLVLVGIRT
                     GGVYLAERLQKKISGIETGKIPLGILDITLYRDDISTSNKKPRLGKTKINFSLDNKKV
                     VLIDDVLFTGRTIRAAMDALIDFGRPKVVQLAVLIDRGHRELPIRADFVGKNLPSSLW
                     EAVRVDLMEKNGVDEVVIEEGI"
     gene            2749..>3160
                     /locus_tag="VF305_02025"
     CDS             2749..>3160
                     /locus_tag="VF305_02025"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX7415944.1"
                     /translation="MAVNYEPQVFLFLSRILGKKIVGAMGETLGRVYDLTAEFVEPYP
                     VVTGLIFSSAQKRNPVFLPWKDVVDLAADVSVAINSTRELSTLNLRQGELLLKDDLLD
                     KQVVDTDGAKIRRVNDLQFLKTSHGLHLVHVDVGF"
BASE COUNT          819 a          708 c          672 g          961 t
ORIGIN      
        1 aatgacatcc agcgttccat cagccagacc ttctttaata gcctttacat cctgaacgct
       61 ccttaaaggt ggattgactt ttaagtttgt atcgtagtcc tgaagggctt catcggttaa
      121 ggtaaagtaa tggggggcgg tctccgcggt tactttcaaa cctcgttttt ttgcctcacg
      181 aatcaagcgc acggaaccag ttgtacttac atgcgcgatg tggacgcaag tcttggtgaa
      241 ttcggcaatc aaaatatcgc gcgctatcat tgcttcctcc gcgatgctcg ggatgccgcg
      301 tagccccaaa atcgtggaat agtaaccttc gttcatcaag ccgccgccgg aaaggtttat
      361 atcttcacag tgcgaaataa ccggtaatcc caaagaataa gcatattcca aagccctgcg
      421 cattaacgcg gcatccatta ccggttttcc atcatcagaa agagcaacca cacccgcatc
      481 tttcaaatcc caatattccg ttaattggga accggaagaa tccttactga ttgcgccgat
      541 tgggtaaacg ttggctagcg cggcctctac tgcttttctt ctgataaatt cggtaataga
      601 gcggttatca ttaacgggtg atgtgttagg catacaggca atcgaggtaa aacctccggt
      661 aacagcggcc gcaactccgg aagcaaccgt ttctttgtat tcaaaacccg gttcacgcaa
      721 atgggtgtgc atatcgatta agcctggcgt aagaatcatt ccatccagtt taataatctt
      781 tacttttcct gaatccttgg attttgctgg ctttagaaga tctgcaccaa ttttggcaat
      841 ttttccatcc tccagtaaaa tatctatatc ggcatctatt ttttgggcag gatcgattat
      901 cctcgcaccg gctaataata tcctcattca ttacctccgg tcagcaagta aagcagcgcc
      961 attcttaccg ccacaccttt atttacctga tcgagaatta ccgaataaga tggatcgtcg
     1021 gcgatgtctg gcgatatttc cacgccgcgg ttaatcggtc cgggatgcat gacgataaca
     1081 tctttcttgg ccagcttaat gttgttacgg ttaagacagt aatgctggga atattcgcga
     1141 agcgtcggaa aaatgctttg cttttgcctc tccagttgga tgcgcaacat catcaccaca
     1201 tcggcctcaa caatggcgtc ctccagcttg ttgaaaattt ttacacccat ttgttcaatg
     1261 tcccggggaa gcatagtagc gggaccggcc agaaagactt gggcgcccat tttggataaa
     1321 ccgtatatat tcgaacgggc aacccggctg tgggcaatat cgccgacgat tgccactttc
     1381 aatccggcaa ttttaccttt tttttccttg atcgtcatca tgtccaaaag tgcctgcgtg
     1441 gggtgttcat gagcgccgtc gccggcgttg ataatggatt gcttaaccag tgatgccagt
     1501 aagtgcggag caccggcagc gctgtggcgg atgacgataa catcgggatt catggcctcc
     1561 aaattacgtg ccgtgtcaat gagtgtttct cccttgacca cagaactggt ggaagcggaa
     1621 atgttgactg tatcggcact gagccttttg ccggcaattt caaaggatgt ccgcgttcgt
     1681 gtgctggctt cgtaaaataa gttgatgatt gttttgccgc gcagtgtcgg cactttcttg
     1741 atttcgcggg tggaaatttc cagaaaagat tctgctgttt ccagaataag attaatctca
     1801 tcaactctaa ggtctttcat gccgaggata tctttttttt ctaatttcat aatcaggttc
     1861 tataatttaa tgtttaaatt ccttcttcaa taactacttc atccacaccg tttttttcca
     1921 tgaggtcaac tctcaccgct tcccagaggg aagacggcaa attctttcct acaaaatcgg
     1981 ctctaatcgg aagttcgcgg tggccgcggt cgattaaaac agcgagttgg accactttgg
     2041 gccggccaaa atcgatcaaa gcgtccattg ccgcgcgaat tgtccgcccg gtaaagagga
     2101 cgtcgtcaat caggacaact tttttattgt ccagggaaaa atttattttt gtttttccca
     2161 gacgcggctt tttgttggaa gtggaaatat cgtcacgata aagagttata tccaggatgc
     2221 caaggggaat tttaccagtt tcaatgccgg atattttttt ttgcaacctt tccgccaaat
     2281 atactccgcc ggttctgatt ccgacaagga cgagattttc tacgccttta tttttttcca
     2341 gaatttcgta agcaatccga gtgaggcatc ggtcaattcc ctctttatcc attactattt
     2401 tagtattttt attttccgcc ataattgttt gacctctatt aagaataaat caatgccaaa
     2461 ccattttcat agggactgcc atttttttga agcgttttta acttcctttg aaattgcgca
     2521 ataaaaaaag cctttcccct tgcggagaaa ggctttatat actttttgat tatttccgtc
     2581 atcgctaatt cctttttcaa tctcgccgga ttaaattaaa aggtttcatt ttttacattt
     2641 tgtattttta tacaactaaa cagcttatgt gtcaaggtgt aaaatatatt caaaaaactt
     2701 ttgtttaaaa aaaataaatg atataaggac acaacctgga ggaaaaatat ggcggtaaat
     2761 tacgaacctc aggttttttt atttttgagt agaattctgg ggaaaaaaat cgttggtgca
     2821 atgggcgaaa cacttggccg tgtttatgat ctcactgcgg aatttgttga gccttatccc
     2881 gtggtcacgg gactgatttt cagctctgcc cagaaaagaa accccgtttt tctgccctgg
     2941 aaagatgtgg tcgatttagc tgcagatgta tccgttgcta taaattcaac gcgtgaatta
     3001 tctaccttaa atttgcggca aggcgaactt ttgctgaaag atgatctttt agataaacaa
     3061 gttgttgata ccgacggagc caaaatcaga agggttaacg atctccagtt tttaaaaaca
     3121 agccacggat tgcatttggt ccatgttgat gttggcttcc
//
LOCUS       DASVIM010000102         3959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1488363, whole genome shotgun sequence.
ACCESSION   DASVIM010000102 DASVIM010000000
VERSION     DASVIM010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488363"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1103
                     /gene="queA"
                     /locus_tag="VF305_02030"
     CDS             63..1103
                     /gene="queA"
                     /locus_tag="VF305_02030"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX7415945.1"
                     /translation="MELKDFSYFLPDELIAQHPCQQRDRSRMMLLCRNDEKIEDFHFF
                     QLPDFLQSGDVLVINDSRVIPARLFGKKPTGGILEILLLTKKESKEKYQTWEVLVRPA
                     KRLRENDVIALGNNCEAKVLARISDKKWLLDFFTPDGFEDYLNRFGRAPLPPYIKRKK
                     TVAMDTTNDRERYQTVYAKSPGSIAAPTAGLHFSADVLQALKAKGVQIASITLHVGLG
                     TFLPIEANEIENHVMEAEYYEISADAAHLINNARRVIAVGTTSTRTLESVADKDGYIK
                     AQSGLTNLFIYPGYEFKRVTGLITNFHLPQSSLFLLVCAFAGTSFIKKVYQHAVENHY
                     LFYSYGDCMLIL"
     gene            1115..2227
                     /gene="tgt"
                     /locus_tag="VF305_02035"
     CDS             1115..2227
                     /gene="tgt"
                     /locus_tag="VF305_02035"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:NF013835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX7415946.1"
                     /translation="MPFKFELIKKDNSTSARLGRMITYRGTVNTPAFMPVGTQGTVKS
                     MLPEEIKNCGTEIILANTYHLYLRPGHETIKKLGGLHRFMNWPNPILTDSGGFQVYSL
                     GALRKIQSDGVIFRSHIDGSKHFLTPQKAVEIQEALGSDIMMCLDECTPYPATPKQTQ
                     ESLALTVAWAKCCREAKTNNDQALFGIIQGGTYLDLRRQSVEETVPIGFDGYALGGVS
                     VGEPKEIMYRIADTITPMLPEEKPRYLMGVGTPEDIVYGVSCGIDMFDCVIPTRCARH
                     GLLFTNSEKIVIKNSRWRENNAPLDETCDCYTCKNYSRAYLRHLFVAGEILAMVLNTI
                     HNVRYYMRLMEKIRAAINEENFLEFKNEFLTKKGGI"
     gene            2229..2564
                     /gene="yajC"
                     /locus_tag="VF305_02040"
     CDS             2229..2564
                     /gene="yajC"
                     /locus_tag="VF305_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX7415947.1"
                     /translation="MTSLAYAMGGSPGGSAGGGDWGFIITMVVIFVIFYFLLIRPQQK
                     KQKELKILLGNLAYGDTIMTSGGIHGKITGLADAVITLEIADKVRIKISRSAVASIIQ
                     KAGAAAPKE"
     gene            2583..>3959
                     /gene="secD"
                     /locus_tag="VF305_02045"
     CDS             2583..>3959
                     /gene="secD"
                     /locus_tag="VF305_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX7415948.1"
                     /translation="MIKSLKIRMIITAIVCFVALYFLVPTFITQIPSPWNDYFPKEKI
                     HLGLDLQGGMHLVLEVDTEKALESMVERMANDLKESMMESKVRFRKVEKAKGATISLE
                     LTESAGKTALDKILNDQYPDLEIASSSPREDAQIVHIKIKDKRAIDLKKLTVEHSLET
                     IRNRVDQFGIAEPEIIPEGDNRILIQLPGIKDPERAKNLIGKTALLEFKIVDDEHPLE
                     DALRGNIPEGDIISYGLRADRSTGQKGSVPYLLKNKALLTGASLETAKVQISDRFGEP
                     NVSIKFNSQGATDFDRITGENVRKHLAIVLDGVVHSAPVIQERISGGQAQITGNFTMD
                     EGRDLAIVLRAGALPAPVNILEERTVGPSLGSDSIRQGIMATLIGSLLVILFMIIYYR
                     LSGAVADLALIINIVLVLGVLVAFKATLTLPGIAGLLLTVGMAVDANVLIFERIREEL
                     RTGKTIRLS"
BASE COUNT         1166 a          861 c          876 g         1056 t
ORIGIN      
        1 ttgacataag cattgcaatt tgcttatttg ccccaacttt ttttctatgc gcaaagaggt
       61 atatggaatt aaaagatttt tcatattttc tacccgatga acttattgcc caacaccctt
      121 gccaacaaag ggaccgctca cgcatgatgt tgctttgccg caatgatgaa aaaattgaag
      181 attttcattt ttttcaacta cctgattttt tacaaagcgg tgatgtcctg gtcatcaatg
      241 attcccgtgt tattcccgca agactctttg gcaaaaagcc gaccggaggt attctggaga
      301 tattgctttt aactaagaaa gaaagcaaag aaaaatacca aacatgggaa gtcctggtaa
      361 gacccgctaa gaggctgcgc gaaaacgatg ttatcgctct cggtaataac tgtgaggcca
      421 aagttttagc ccgcatatct gataaaaaat ggctattgga tttttttact ccggatggtt
      481 ttgaagatta tttaaaccgt ttcggacgag cgcctctgcc accttacatc aagcgcaaaa
      541 aaaccgtggc aatggatacg acgaatgacc gggaacgcta tcagacagta tacgcgaaaa
      601 gtcccggttc gattgccgct ccaactgccg ggctacattt ttcggcagat gttcttcaag
      661 cactaaaggc aaaaggggtg caaatcgcat ctataacttt acacgttggc ttagggactt
      721 ttttgccaat cgaagcaaat gaaattgaaa atcatgttat ggaagcagaa tattatgaaa
      781 taagtgcaga tgcagcccac ttaattaaca atgccaggag ggtaattgct gtaggaacaa
      841 cttcgacaag aacattagag agcgttgccg ataaagatgg atatatcaaa gcccaatcag
      901 gccttactaa tctctttatt tatcccggct atgaattcaa aagagtcacc ggactaataa
      961 caaattttca tttgccgcaa tcatccttat ttttgcttgt ttgcgctttt gccggtacaa
     1021 gctttattaa aaaagtatat cagcatgcag tagaaaacca ttatctcttt tacagttatg
     1081 gtgactgcat gctgattctt taggatattt ataaatgcct tttaaattcg aactgatcaa
     1141 gaaagacaat tcaacttctg cccgtttggg taggatgatc acttaccgcg gcacggtcaa
     1201 tacgccggct tttatgccgg taggcacgca agggactgtc aaatctatgc tccccgaaga
     1261 aatcaaaaat tgcggtaccg aaatcatttt ggccaacact taccaccttt atttacggcc
     1321 cggccatgaa acgatcaaaa agctgggtgg tcttcatcgt ttcatgaact ggccgaaccc
     1381 aattctgacc gatagcggcg gttttcaggt ttactctctg ggagcgctgc gtaaaataca
     1441 gtccgacggc gtgatatttc gttcccatat cgacggttcc aaacactttc ttactccgca
     1501 aaaagctgtt gaaattcagg aggcgctggg ctcggatatt atgatgtgcc tcgatgaatg
     1561 taccccctac cctgccacac ccaaacagac tcaagaatca ctggctttaa ccgttgcctg
     1621 ggcaaaatgt tgccgcgagg caaaaacaaa taatgatcag gcactcttcg gtattattca
     1681 gggaggcact tatctggatt tacgcagaca gtcggtcgaa gaaaccgtac ccatcggttt
     1741 tgacgggtat gccctgggcg gagtcagtgt gggggaaccc aaagaaatca tgtatcgtat
     1801 tgccgatacc attacaccca tgttgcctga agaaaaacct cgttatctaa tgggcgttgg
     1861 cactccggag gatattgttt atggagtttc gtgcggaata gacatgtttg actgcgttat
     1921 accgacacgt tgcgcccgtc acggattatt gtttacaaat tccgaaaaga ttgttataaa
     1981 aaattcccgc tggcgggaaa ataatgctcc gcttgatgaa acatgtgatt gctacacttg
     2041 taaaaattac tctcgagcat atttgcggca tctttttgtt gccggtgaga tactggctat
     2101 ggtattaaac actattcaca atgtccgcta ctatatgcgt cttatggaga aaattagagc
     2161 agcaataaat gaagaaaatt ttttagaatt taaaaatgaa tttttgacaa aaaaaggagg
     2221 aatttaaatt gacatcttta gcatacgcga tgggtggtag tccgggtgga tcggccggtg
     2281 gcggcgattg gggtttcatc atcaccatgg tagtaatttt cgtgattttc tattttttac
     2341 taattcgccc tcaacaaaag aagcaaaagg aactcaaaat cctgttaggt aaccttgctt
     2401 acggcgatac gatcatgacc agcggcggga ttcacggcaa aataaccggt ttagccgacg
     2461 cagtaatcac tttagaaatt gccgacaaag taagaattaa aatctcccgt agcgctgtgg
     2521 cgtccatcat tcaaaaagcg ggagcggccg caccaaagga atagcataga aaggggcaat
     2581 tcatgatcaa atctttaaaa atcaggatga taatcacggc aattgtgtgt ttcgttgctc
     2641 tgtatttttt ggtcccgact ttcattaccc agattccctc tccctggaat gattattttc
     2701 ccaaagaaaa aattcatctg gggctggatt tgcaaggtgg aatgcatctt gttttagaag
     2761 ttgatacgga gaaggctcta gaatcaatgg tggaaagaat ggccaatgat cttaaagaat
     2821 caatgatgga aagtaaagta cgtttccgga aagtggaaaa ggcaaaaggc gccacaattt
     2881 ctttggagct gactgaatcc gcaggaaaga ccgctttgga taaaatcctc aatgaccagt
     2941 atcctgattt agaaatagcc tcttcctccc cgcgtgaaga cgcacaaatt gtccatataa
     3001 aaatcaagga taaaagagca atagacctca aaaaactaac cgtcgaacat agtctggaaa
     3061 caattagaaa cagagttgac cagttcggca ttgctgaacc ggaaattatc ccggaaggcg
     3121 acaaccgcat tttaattcaa ttgccgggca ttaaagaccc ggaaagagca aagaacctca
     3181 tcggaaaaac cgcccttctg gaattcaaaa tagttgacga cgaacacccg ctggaagatg
     3241 ctctccgcgg caatattccg gagggagaca taatctctta cggtttgcgt gccgacagat
     3301 caaccggaca gaaaggttcc gtaccatatt tgttgaaaaa caaggcgctt ctaaccggtg
     3361 cctctttgga aaccgccaaa gtgcaaattt ccgaccgttt cggcgaacca aatgtgtcta
     3421 taaaatttaa ttcccaaggt gctaccgact tcgatcggat taccggcgaa aatgttcgca
     3481 aacacttagc tattgtcttg gacggcgtag tccattccgc acccgtcatc caggaaagaa
     3541 tatccggcgg tcaggcgcaa attaccggta acttcaccat ggatgaaggg cgcgacttag
     3601 cgatcgtttt acgcgccggc gcactacccg caccggtcaa tattctggaa gaaagaacgg
     3661 ttggtccatc actcggcagc gactcgatta ggcagggtat tatggccaca ctcatcggct
     3721 cactattggt tattttattc atgatcatct attaccgcct ttcgggagct gtcgcggatt
     3781 tagccctcat tattaacatc gttttagttt tgggcgtact ggtggctttc aaagccacgc
     3841 taacattgcc cggaattgca ggtctgctgc tcactgtcgg tatggcggtt gatgccaacg
     3901 tgctaatttt tgaacgaatc agagaagaac tacggacagg aaaaacgatt cgactgtca
//
LOCUS       DASVIM010000103         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_463998, whole genome shotgun sequence.
ACCESSION   DASVIM010000103 DASVIM010000000
VERSION     DASVIM010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463998"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1086
                     /gene="dusB"
                     /locus_tag="VF305_02050"
     CDS             97..1086
                     /gene="dusB"
                     /locus_tag="VF305_02050"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX7415949.1"
                     /translation="MFAGINSIKGKALLAPMAGISNLPFRLIARSFGCTLAFTEMISA
                     NGLVRKTDKTFEYLRTCVEDKPLGVQIFGADPDIMAQAASIVANNGAELIDINMGCPV
                     KKVIRAGAGAILMKDPNRVGSIIKAVRKAVTIPVTVKIRSGWSGRSINAVEIAHVAEN
                     SGADAITIHARTADQGFSDVADWRVIAEVKKTVNIPVIGNGDIWQPQDAIKMLRETSC
                     DAVMVGRGALGNPWIFKGIKQIISGHKEDYRPELAQRLEIIKKHWEMETEFSGKKVAE
                     RNFRKHLLWYTKGLEGSSRFRQLVGKLRDWESIMDELNEYFWSLQELQTKIMP"
     gene            1176..1406
                     /gene="zapB"
                     /locus_tag="VF305_02055"
     CDS             1176..1406
                     /gene="zapB"
                     /locus_tag="VF305_02055"
                     /inference="COORDINATES: protein motif:HMM:NF017786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HEX7415950.1"
                     /translation="MELGKFKELEDKIKGIVSEKVFLKKQIQILEEALKNKEAEFEGL
                     NGKLKALDEERNSVRARVDSLLDLLADIDLVK"
     gene            1425..1703
                     /locus_tag="VF305_02060"
     CDS             1425..1703
                     /locus_tag="VF305_02060"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX7415951.1"
                     /translation="MKKSYKIRILGQELSVLSDAEDEQVANVVQFVNKKIDEVLLSRD
                     DLKTLSVAILAALNISEEFLKLEVVNKELCDQLESRAEKLIQLIEDAS"
     gene            1714..1911
                     /gene="ssrS"
                     /locus_tag="VF305_02065"
     ncRNA           1714..1911
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VF305_02065"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2127..>2674
                     /locus_tag="VF305_02070"
     CDS             2127..>2674
                     /locus_tag="VF305_02070"
                     /inference="COORDINATES: protein motif:HMM:NF023495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HEX7415952.1"
                     /translation="MFNGSLFMLIIVILVAIIAGTVAGFILKQLLADKKIKSSESLAA
                     RIVEEARKEAENIKKEAILQAKENLLKMKADFDRETKDKKSDLDGLEKRIRSKEENLD
                     KRIDSLAQKEANIEGREKSIISKETAIEEKYRRLDSILEEQKEKLERIAEISAEEAKK
                     LLLQSMEAEAKLDAAAIIRKIE"
BASE COUNT          904 a          444 c          646 g          680 t
ORIGIN      
        1 cctgattata tcgaagtaat gggaattaac acgccggaag aactctctct cgccggacag
       61 tatcttcaaa agaatagaaa atatctggtt taatttgtgt ttgctggcat taattcaata
      121 aaaggcaaag ctctattggc gcctatggcg ggaatttcca atttaccgtt tagattgata
      181 gcgcgttctt tcggctgtac cttagccttt actgaaatga tcagcgccaa cggacttgtc
      241 cggaaaacag acaaaacttt tgaatacttg agaacttgcg tagaggataa acctttaggt
      301 gttcaaattt tcggagctga tccagacata atggctcaag ctgcaagtat tgttgccaat
      361 aacggcgccg aattaataga catcaatatg ggttgcccgg taaaaaaggt aatcagggcc
      421 ggagccggtg caattcttat gaaagatcct aaccgcgtag gcagtattat caaagcagta
      481 agaaaagctg taaccattcc tgtaaccgta aagatacgtt ccggctggag cggccgctca
      541 atcaatgccg tggagattgc gcatgttgct gaaaattccg gagccgacgc aattaccatt
      601 cacgcccgca cggccgatca gggtttcagc gacgtagccg actggagggt cattgctgaa
      661 gtaaaaaaaa cagttaatat tccggtgatc ggcaatggag atatttggca gccgcaggat
      721 gcaataaaaa tgttgcggga aacttcctgc gacgcagtga tggtcggaag aggagcctta
      781 ggcaatccct ggatatttaa agggattaaa cagataattt ccggccataa agaagattat
      841 cggccggaat tagcccagcg tctggaaata attaagaaac attgggagat ggaaaccgaa
      901 tttagcggga aaaaggttgc ggagcggaat tttcgtaaac atcttctttg gtatacgaag
      961 ggattagagg gttctagccg gttcagacaa ttggtaggga aattgaggga ctgggaaagc
     1021 ataatggatg aattaaatga atatttctgg tcgctgcagg agctgcaaac taaaataatg
     1081 ccttgacatt aagatcatta attggcataa attagaaaca aatttaagag cattacctgc
     1141 tagtttttat gtatttggaa ggatagaaat ttacggtgga attgggaaaa tttaaagaac
     1201 tagaggataa aattaaaggt attgtcagtg aaaaagtttt tttaaaaaag caaattcaaa
     1261 tattggagga agcattaaaa aataaagagg cggaatttga aggtttaaac ggtaaattaa
     1321 aggcattaga tgaggaaagg aacagcgtgc gcgcaagggt agattcactg ctcgatttac
     1381 ttgcagatat agatttagta aaataagctt ccagggcgtg agttttgaaa aagagttata
     1441 aaataaggat attgggacag gagctttcgg ttttaagtga tgcagaagat gaacaagtgg
     1501 ccaacgttgt ccaatttgta aataaaaaaa ttgacgaggt tttgctttcc agagatgatc
     1561 tcaaaacctt gagcgttgcc atcctggcag ctttaaacat ttcagaggaa tttttaaaat
     1621 tagaggtagt taataaggaa ctctgcgacc aactggaaag cagagccgaa aaactaattc
     1681 aactgattga ggatgcaagt tagtaaattt tattcgcccc tgcgatgcgc gtgattaaca
     1741 tttgtttttg atccaacatt ttgaatctgg ggcctttacc ttgtttgcgg tgtgcaggtc
     1801 agcctttttt cccgctggtc gggaggcaga aagcctaaaa caaacaatac cgggcgccca
     1861 ctttgtaaaa aggttcaaaa aggtgattaa cacggcatag gcgggggttt ttttatgccc
     1921 ccagtgtcgc aaaatcactt ccgcctttag agaaaaaatc aattaacaat aaatccttcg
     1981 gtgaagtgtt taaaattaaa cgcttactga gagttttaaa tatagctccg ctggagataa
     2041 gttttactgt aaaaaaggcg ataaaattat caagcgaata ggttgaggga cggattttcc
     2101 agaggagcaa gaggaggtaa agttcaatgt ttaatggcag tttattcatg ctgattatag
     2161 ttatattggt ggcaatcatt gccggtactg ttgccggctt tattttaaaa caattgctgg
     2221 ccgacaaaaa gatcaagtca tcggagagct tagcagcgcg gatcgtcgag gaagctagaa
     2281 aagaagcgga gaatatcaaa aaggaggcta tccttcaagc caaagaaaac cttctgaaaa
     2341 tgaaagctga ttttgaccgg gaaacgaaag ataaaaaaag tgatcttgac ggtttggaaa
     2401 agcgcattcg ttccaaagaa gaaaatctgg ataaaaggat tgattcatta gcacaaaaag
     2461 aagcaaatat cgaagggcgt gaaaaatcaa taattagcaa agaaacggcg attgaagaaa
     2521 agtaccgcag attagactct attctggagg aacaaaaaga aaagctggaa agaatagcgg
     2581 aaatttctgc tgaagaagcg aaaaaattat tgctccagtc gatggaagcg gaggccaaac
     2641 ttgatgccgc cgcaataatc cgcaaaatcg aaga
//
LOCUS       DASVIM010000104         3441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1490365, whole genome shotgun sequence.
ACCESSION   DASVIM010000104 DASVIM010000000
VERSION     DASVIM010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490365"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /gene="queE"
                     /locus_tag="VF305_02075"
     CDS             complement(<1..538)
                     /gene="queE"
                     /locus_tag="VF305_02075"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEX7415953.1"
                     /translation="MSYLIKEIFYSIQGEGFHSGRPAVFCRFAGCNLWSGKEVDRKDA
                     LCKFCDTDFMGINGPEGGRFQSAGELAKKVRQLWPEKDLKSAKPFVVCTGGEPLLQLD
                     AQLIAALHEEGLEIAVETNGTIMPPPDIDWICVSPKAGAELLLRSGHELKLVFPQTGA
                     KPEKYDKLDFQYFFLQPMD"
     gene            complement(549..1247)
                     /gene="queC"
                     /locus_tag="VF305_02080"
     CDS             complement(549..1247)
                     /gene="queC"
                     /locus_tag="VF305_02080"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX7415954.1"
                     /translation="MTNRKQKAVVLLSGGIDSTTTLAIAKNMGFAIYALSFRYGQRHE
                     VELKAASRIARHFNIAEHLIVDIDLRRIGGSALTGDIAIPKSRDIKQMGKDIPVTYVP
                     ARNTIFLSYALAWAETIKAFDIFIGVNALDYSGYPDCRPEYIAAYEKMANLATKAGVE
                     GKQRIKINTPLIRMTKAQIIRKGIELGVDYSQTHSCYDPSAEGVACGECDSCLLRLNG
                     FQEAGIKDSIRYKI"
     gene            complement(1272..2051)
                     /locus_tag="VF305_02085"
     CDS             complement(1272..2051)
                     /locus_tag="VF305_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7415955.1"
                     /translation="MLIDSHAHLEMKEFDFDREEVIERAGQAGVDFIVTVGTNLALSR
                     KAVELAEKHENIYATVGIHPHDVAKTDKNTYEALKELTHRQKVVAYGEIGLDFFRNIS
                     PQKEQIDLFGRQLELANELKLPVIIHDREAHEQTLRMVKASGVRCGVFHCFSGDYAMA
                     RQCIDLGFYISIPGVVTFDKAKTIQDVAQRVPLSSLLLETDAPYLAPIPHRGKRNEPS
                     FIIHTAKKVAELKGVSLEEVAQATTKNAKSLFHIEASGNIK"
     gene            complement(2058..2225)
                     /locus_tag="VF305_02090"
     CDS             complement(2058..2225)
                     /locus_tag="VF305_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415956.1"
                     /translation="MSKKVNAELLNLDRRIISRKLLLGEISEKDLQSFLKKIPDVADN
                     AEEVYPDGNEK"
     gene            2427..2957
                     /locus_tag="VF305_02095"
     CDS             2427..2957
                     /locus_tag="VF305_02095"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7415957.1"
                     /translation="MTKLIIFFAVSIIFIKISWPSLFDTGSRKFYRFFAFEFLLLLFL
                     VNSSYWLSKPLSFFHIISWVLLAISLLMAFRSMYHLLNDGKPAGTGNIETTTNLVTSG
                     MYKHIRHPLYSSLLFLSAGLFFRNVSLLATSLFIVIAAFIYATARIEENENSLKFGVD
                     YVFYRKKSKMFVPFLF"
     gene            2989..>3441
                     /locus_tag="VF305_02100"
     CDS             2989..>3441
                     /locus_tag="VF305_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415958.1"
                     /translation="MQLNLKPGKEILLRLGENDIRKSQIHELLPNDFISIEQTQPPIQ
                     NVHLNQIVLFTYLSAAEKPGRFGLEARIQEVTADNRIILHKLNDPAPCDLRVWPRICL
                     DSLPDIHAYCKEKEIHVIDISCRGTHIILPKDDGEEPEIEAVIKMKLVL"
BASE COUNT          945 a          804 c          653 g         1039 t
ORIGIN      
        1 tatccattgg ctgcaaaaaa aagtactgga aatcaagctt atcatatttt tccggcttcg
       61 cacccgtctg cggaaagacc aattttaact catggcctga gcgtaacaac aattcagcac
      121 cggctttggg actgacgcaa atccaatcaa tatcaggcgg cggcatgatt gtgccgttgg
      181 tctccacggc aatttccaaa ccttcttcat gtaacgcagc aatcaactgc gcatccagtt
      241 gcagaagcgg ctcacctcca gtgcagacca caaaaggctt tgctgatttt aaatcttttt
      301 ccggccataa ttgcctcacc ttttttgcta attctcctgc actctgaaaa cgcccgcctt
      361 cgggtccatt aatgcccata aaatccgtat cgcaaaattt acacagggca tctttacggt
      421 caacttcctt tcccgaccat aaattgcacc cggcaaaacg gcaaaagacc gccggacggc
      481 cagaatgaaa accttcccct tggatcgagt aaaatatttc cttgatcaga taactcaaaa
      541 ttgacctttt agattttata cctaatagag tctttaatgc ccgcttcttg aaaaccattt
      601 aaacgtaaaa ggcaggagtc gcattctcca caggcaacgc cttctgccga tggatcatag
      661 cagctgtgtg tttggctata atcaacaccc aactctattc ctttacggat aatttgcgct
      721 ttggtcatcc tgataagcgg cgtattaatt tttattctct gcttaccttc cacgccggcc
      781 tttgtggcca gattggccat cttttcataa gccgcaatat attcgggacg gcaatcggga
      841 tagccgctat aatccagagc attaacaccg ataaatatat caaatgcttt tattgtttcc
      901 gcccaggcca gagcgtaaga aagaaatata gtattgcgtg ccggaacata agttaccgga
      961 atatcttttc ccatttgttt aatgtcgcgg ctcttaggaa tagcaatatc ccctgttaag
     1021 gctgaaccgc cgatgcggcg cagatcaata tcgacaatca gatgctcggc tatgttaaaa
     1081 tgccgggcaa tgcgggatgc tgctttcaat tccacctcat gacgctgacc atacctgaag
     1141 ctcaaggcat agattgcaaa tcccatattt ttggcgatag ctaatgttgt ggtggaatcg
     1201 ataccgccgc ttaataggac aacagctttt tgtttacgat tcgtcatcgc gcaactcttt
     1261 tttgatatgt tctatttaat attgccggaa gcttcaatat ggaaaagact cttggcgttt
     1321 ttggttgtgg cttgggccac ttcctccaaa gacacacctt taagctccgc tactttcttt
     1381 gccgtatgga taataaaaga aggttcgttt cttttaccac ggtgcggaat aggcgcaaga
     1441 taaggcgcgt ccgtttccag aagtagtgaa gaaagcggca cacgctgtgc gacatcctga
     1501 attgtcttgg ccttatcaaa ggtcaccacg ccggggatgg agatgtaaaa tccaaggtct
     1561 atacattgtc tggccatggc ataatcgccg gaaaaacaat gaaagacacc acagcgcaca
     1621 ccggaggcct taaccattct cagggtttgc tcatgggctt cacgatcatg aataatcacc
     1681 ggcagtttca gctcattagc taattccagc tgccggccaa acaaatctat ttgttctttt
     1741 tgaggtgaaa tgttgcggaa aaaatctaac ccaatttcac cataggcaac aactttttgc
     1801 ctatgtgtca gctccttaag agcttcgtag gtgttcttat ctgtttttgc cacatcatgg
     1861 ggatggatac cgactgtcgc gtaaatattt tcatgctttt ccgcaagctc aaccgctttg
     1921 cggcttagcg ccaagtttgt tcccacagtg acaataaaat caacgcctgc ctgcccggca
     1981 cgctcgatca cttcctcacg gtcaaaatcg aattctttca tttccaggtg ggcgtgggaa
     2041 tcaatcaaca tacgcagcta tttttcgttt ccatccggat aaacttcctc agcattatcg
     2101 gcaacgtcgg ggattttttt taaaaagctt tgtaaatctt tttcggaaat ctcaccgagt
     2161 aataacttac gcgaaataat gcgcctatcg aggttgagta attctgcatt aacttttttg
     2221 gacatgttaa gctttctcct tctacactta attgaacgag ccgactacta ttacattttc
     2281 tcaattttat caagtctcaa ggcataaact tcggatcatt tattacggga aataccttga
     2341 caccggacaa cctttccgct aaatattaca atgattttaa agcgattacc ctaatataga
     2401 gcttattttt atttgatagg caacttatga ctaaacttat tatatttttt gcggtttcta
     2461 taatatttat caaaatttcc tggccgtctt tattcgatac cggcagccga aagttttacc
     2521 ggttttttgc ttttgagttc ctcctccttc tatttttagt caatagcagc tattggttga
     2581 gtaaacctct ttcttttttt cacataattt cgtgggtgct tttagccatt tccctgctta
     2641 tggcttttcg cagtatgtat catttactca atgatggcaa acccgccggc accggcaaca
     2701 tcgaaacgac taccaattta gtaacctccg gcatgtataa acatatccgc catcctcttt
     2761 atagttcact tctatttctt agcgcaggat tatttttcag aaatgtttca ctacttgcca
     2821 ctagtttgtt tatcgttatc gcagcattta tttacgccac agcgcgaatc gaggaaaatg
     2881 aaaactcact taaattcgga gttgattatg tcttctaccg caagaaatcg aaaatgtttg
     2941 ttcctttttt attttgaacc accatcaaaa agaggatatt aataactaat gcaattgaac
     3001 cttaagccgg ggaaagaaat attattacgc ttgggtgaaa acgacatccg taaatctcag
     3061 attcatgagc ttttacccaa tgattttatt tctattgaac aaactcagcc acccatacaa
     3121 aatgtgcact tgaaccagat tgtccttttt acttacctgt cagccgcaga aaaaccagga
     3181 cgtttcggtt tggaagcgag aatccaagaa gtcacggcag ataaccggat tatcttgcac
     3241 aaattaaatg acccggctcc ctgtgattta agagtctggc caagaatctg cctcgattca
     3301 cttccggaca tccatgctta ttgtaaggaa aaagaaatcc atgttataga tatatcctgt
     3361 cgcggcactc atattatatt gcctaaggat gatggcgaag aacctgaaat tgaggctgtc
     3421 atcaagatga aattagtttt a
//
LOCUS       DASVIM010000105         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1661444, whole genome shotgun sequence.
ACCESSION   DASVIM010000105 DASVIM010000000
VERSION     DASVIM010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661444"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..940)
                     /locus_tag="VF305_02105"
     CDS             complement(263..940)
                     /locus_tag="VF305_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7415959.1"
                     /translation="MERNFAKLNYYEILDVKPEAAFFEIRHAYNTALQMYQSGSLVSH
                     SFFSQKEREEILALIEKAYLMLANEKERKNYDNELIRLGILKATGKKVAAKKPVSIFD
                     SNRQRDRASMLKNNNAELKAKIAQNQRIREILSQQEISGTDFKAIRNELGVAIEKIAQ
                     ETKIRIDCLNNIEENNIKKLPVAVFLKGFIKSYLKCLCIEPADDICALYMSGLARIGK
                     NNGSGVF"
     gene            complement(1001..>1511)
                     /locus_tag="VF305_02110"
     CDS             complement(1001..>1511)
                     /locus_tag="VF305_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415960.1"
                     /translation="NSIFITTPEPTSIENVYRLVRSIYFRRIRQYFNAADFKALEEEV
                     IQQFGDGSFNKPDCIMQAVKRAYPEKSLQLENDFSSFRFKLVLNQLRKNDNPALGSQI
                     CKIIEKHLGLQVDFAGNVAFDDHVHDIICQRASFLDRYPYTRAAFDLRELGKQIVRST
                     GEQLLIRYS"
BASE COUNT          435 a          318 c          269 g          489 t
ORIGIN      
        1 cgaaaaatat cattatggta ccatggccca caaatgtgat gcccagattg cctccaggcg
       61 ttttgaacgt gtcggattcg aactgttgtt cagccgcaac tgccatagtg gtgcagaata
      121 agaataagct cgtataagca aaaattattt ttatcataat ttccctcctt ttttctgatt
      181 taataaacgc ggattgcttc gtttactttc cctgggcagt cgtttaaaag cgcctatttt
      241 caatccatca ggaactcgaa ttttaaaata ctcccgaccc attatttttc ccaatgcgtg
      301 ccaagccgct catgtaaagg gcacagatat catctgccgg ttcaatacag aggcatttta
      361 agtaggattt aatgaagcct ttgagaaaaa ctgctacagg cagtttctta atattatttt
      421 cctctatgtt gtttagacaa tcgatacgga tttttgtttc ttgagcgatt ttttcaatgg
      481 ctacacccaa ctcatttcta attgccttaa aatccgtgcc gctaatttcc tgttgggaaa
      541 gaatttcacg aatacgttga ttttgagcaa tttttgcttt taattcagcg ttattatttt
      601 tcagcatgct cgccctatcc cgttgtcgat tactatcaaa aatgctaacc ggctttttag
      661 ccgccacttt ctttccggta gctttgagaa ttcccaaccg gataagttcg ttgtcgtagt
      721 ttttcctttc tttttcatta gcgagcataa ggtaagcttt ttctataagg gcaagtattt
      781 cctctctttc tttttgcgaa aaaaaagaat gactaaccag agagcctgac tgatacattt
      841 gcaaagcggt gttataagca tggcgaattt caaaaaatgc cgcctccggc ttaacatcca
      901 gtatttcata ataattcagc ttagcaaaat ttctttccat gcaagacctc aactactata
      961 ttactatatt ttttgtcatc tttagatatg atcgtaaact ttaagaatag cgaatcagca
     1021 attgctcgcc ggtagaacga acaatttgtt tgcctagttc acgcagatca aaagcggctc
     1081 gcgtatatgg ataccggtct aaaaacgatg cacgctgaca aatgatatca tgaacgtggt
     1141 catcaaaagc aacgttaccg gcaaaatcaa cctgtaaccc aagatgtttt tcaataattt
     1201 tgcatatctg cgaacccaaa gcaggattat cattttttcg caattggttg agcaccagtt
     1261 tgaatctaaa agagctgaaa tcattttcca gttgtaaact tttttccggg tacgctcttt
     1321 taaccgcctg catgatgcaa tccggcttat taaaagaacc gtcgccaaat tgttgtataa
     1381 cttcttcttc cagagcttta aaatcagccg cattaaaata ctgacgaatc cggcggaaat
     1441 aaatggaacg aaccagccgg taaacgtttt caatagaagt aggttccggc gttgtaataa
     1501 atatactgtt t
//
LOCUS       DASVIM010000106         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_296299, whole genome shotgun sequence.
ACCESSION   DASVIM010000106 DASVIM010000000
VERSION     DASVIM010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296299"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VF305_02115"
     CDS             <1..775
                     /locus_tag="VF305_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX7415961.1"
                     /translation="TAEEALKLNVIDFVAPDIDKLLAAIDKKEVNLAKGKKKISTKDA
                     IINSKKMGTRQGILAAISDPNISYILLLIGLAGLYFELSTPGAILPGVIGGISLLLAF
                     FGLSTLPVNYTGILLIIFGVILFFAEIKVMSHGLLTVGGVISLVMGSLLLFDTAEPAL
                     RISLQVLIPAVLVVSGFFIVVIWIAIKAQLRKHLTGAEAIVGTETEALTDIANEGKVF
                     LKGQYWKAISEKLVKKGAKVKIVKVEGLSLIVEEIKKEQ"
     gene            802..1566
                     /locus_tag="VF305_02120"
     CDS             802..1566
                     /locus_tag="VF305_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX7415962.1"
                     /translation="MFGAISIPMIVVIVLVFMFLASAIRILNEYERGVIFRLGRVIAT
                     KGPGIIILIPIVDKMVKVDMRTITMEVPPQDVITRDNVSIKVNAVVFYTVLDATAAII
                     KVENYLYATSQQAQTSLRSVCGQVELDEILSEREKINLTLQEILDRSTEPWGIKVDRV
                     EVKQIDLPEEMKRAIAKQAEAERERRAKVINAAGEFQAAQKLVDAAALMETQPMSLQL
                     RYLQTLNQIAAENNSTTVFPIPIALFKPFLKLAEKL"
     gene            1626..1940
                     /locus_tag="VF305_02125"
     CDS             1626..1940
                     /locus_tag="VF305_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415963.1"
                     /translation="MASSKRHVLTGGMILITLGVLIYLAKASIYPFGQTWPILIIVIG
                     ICTVIQRVKDFGGWFITVAGVVFLINEFYGFELSKYSKYILPAILVLLGIFVLLRRKK
                     HD"
BASE COUNT          607 a          342 c          488 g          584 t
ORIGIN      
        1 aactgctgaa gaagctctca agctgaatgt catcgatttt gtcgcaccgg atatagataa
       61 acttttagcg gcaatcgata agaaagaagt aaatctggcc aagggcaaaa agaaaatttc
      121 aacaaaagat gctataatta acagtaaaaa gatgggaaca cgccaaggaa tcctcgcggc
      181 tatttccgat cccaatattt cttatattct tttgcttatc ggtctggcgg gtttgtattt
      241 tgaactctcc acgccgggag caattttacc cggagtaatc ggagggatat cgctgctctt
      301 ggcttttttc ggcctatcca cattgccggt aaattatacg ggaattttgc tgataatttt
      361 cggcgttatt ttattttttg cggagataaa agttatgagt catggcctgc ttactgttgg
      421 tggtgtaatt tctttggtta tgggatcact gcttttattc gatacggcgg agccggcgtt
      481 gcgtatttct ttacaggttt tgattcccgc cgttttagtg gtttccggct tttttattgt
      541 tgttatctgg atagctataa aagcgcaatt acggaaacac ttgaccggcg ccgaagcaat
      601 tgttggaact gaaactgaag cgctgaccga cattgccaat gaagggaaag tatttttaaa
      661 aggacaatac tggaaagcga taagtgaaaa gctggtaaaa aagggagcaa aagtaaaaat
      721 tgtcaaagtt gaaggtttga gcctgatagt ggaagaaata aagaaagaac aataatcata
      781 aattaaagga gggaatttaa aatgtttgga gcaatatcta taccgatgat agttgttatt
      841 gtgctggtat ttatgttttt ggcctcggcg attcgtatct taaatgaata tgaaagaggc
      901 gtcatctttc gtctggggag ggtgattgct acaaagggac ccggcattat tattttgata
      961 cctattgtgg ataaaatggt caaggtggat atgcggacaa ttaccatgga ggttccaccg
     1021 caggatgtca ttacccggga caatgtgtcc attaaagtca acgccgtagt attctacacg
     1081 gttttagatg cgaccgcggc cattatcaag gtggagaatt atctctatgc gacatctcaa
     1141 caggctcaga ccagcttgag aagtgtctgc ggtcaggtgg aactggatga gattttgtca
     1201 gaaagggaga agattaattt aaccttgcag gaaatcctgg atcgcagtac cgagccctgg
     1261 gggattaaag tcgacagggt cgaagtgaaa cagatagatc tgcctgagga gatgaaaagg
     1321 gccattgcca aacaggcaga agcggagaga gaacggcgcg ccaaggtcat caatgctgca
     1381 ggcgaattcc aggccgcaca aaagcttgtc gatgcagcag ctttgatgga aacacagccg
     1441 atgtcgctgc aattgcgtta cttgcagaca ttgaatcaga ttgctgcaga aaacaattcg
     1501 acaacggtat tccccatacc cattgcttta tttaagccat ttttaaaact agcggagaaa
     1561 ttataaatca gagcggcagg agttaagctt ctgccgctta aaataattat gaggaggata
     1621 tgtttatggc tagttctaaa cgtcacgtgc tgaccggagg tatgatttta attactttgg
     1681 gagtattgat ctatttggct aaggcaagta tttatccttt tgggcagact tggccgatat
     1741 tgataattgt cattggaata tgcacagtaa ttcagagagt aaaagatttt ggcggctggt
     1801 tcataactgt tgcgggagta gtattcctca ttaacgaatt ttatggtttt gaactttcca
     1861 agtactcgaa atatatattg cccgcaatac tggtgttact gggaatcttt gttttattaa
     1921 gacgaaaaaa acatgattga ttaacaagat atcaagtaaa tgaattgtca tttgagaatg
     1981 acaaaactat atattgaaga ttaaaagaaa caggattccg t
//
LOCUS       DASVIM010000107         3398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_297100, whole genome shotgun sequence.
ACCESSION   DASVIM010000107 DASVIM010000000
VERSION     DASVIM010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297100"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VF305_02130"
     CDS             complement(<1..750)
                     /locus_tag="VF305_02130"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEX7415964.1"
                     /translation="MAIDEAIFQETIKNKKPPTIRFYSSHPAAVSIGYFQDLRKEVNI
                     EKCNNMGIDIVRRQTGGKAVFHFEEITYSVIARDEEKLFPPDILGSYKVISKCIAQGL
                     AYLGIKANLDENGRDLTKGKLNSYCFSVSSKNELLVGGRKICGSAQVRRKSGFLQQGS
                     LLLSFDSEKTAALLLPTRTPEQLQKLKKSVAALNEELLAPVDVQEVCFKLKEGFINEL
                     GIELVEESLTLTEETLKNKLMEKYSNARWNIE"
     gene            complement(806..1204)
                     /gene="gcvH"
                     /locus_tag="VF305_02135"
     CDS             complement(806..1204)
                     /gene="gcvH"
                     /locus_tag="VF305_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX7415965.1"
                     /translation="MKVFPEDLLYSCEHIWVRIDGYMATIGITDYAQESLGEILSVEF
                     PEIDTYVERDETFGSIEATKAVVDLISPLNGTVVNVNEDITYDIGIINSDPHDTGWLI
                     VLEMDDLEQLADLLVASSYHDFIIQQEDTD"
     gene            complement(1224..2309)
                     /locus_tag="VF305_02140"
     CDS             complement(1224..2309)
                     /locus_tag="VF305_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX7415966.1"
                     /translation="MPQKNKSLPLRISRLQQNFPAYKIDGLFFLNMSNIRYLSGFTGS
                     DGAFLLSSGNAILLVDGRYTTQAKLEVTNVQVIEYNDKIEGIVQAVKELNLKRIGFEA
                     FSTTVETYNQLTKRLRNKVLVPLSDELKLLRAYKDETEILLMKKAADISSAAILSLLA
                     EIKPGCTEKELALQLELNALQSGADQIAFETIFAAGENSALPHAKPTDRKIKKGDFIV
                     IDFGVKYGGYCSDETCTIALGKLTDKQKNVYQIVKHAHDRTLDFIKADISASDTDNCA
                     RSIFGKKYGRYFSHATGHGVGLEVHEAPRLASNSNDILKPQMVVTIEPGLYIPGLWGI
                     RIEDTVLVKKNSCEKLTKMDKELIIIE"
     gene            complement(2293..2955)
                     /locus_tag="VF305_02145"
     CDS             complement(2293..2955)
                     /locus_tag="VF305_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415967.1"
                     /translation="MNFLDEAEKLLQQNMLPQALNLAEERLQSLPVDVDAHVIAGNAL
                     IGMGRVNEARDILREVEEIISGLSLIYDRMGDIYREKGFHQDAAICYEKFIALNPNAE
                     KAREVIEKMALLEQEDHPVTEVDMPDDNGTLKPDFFTVTLAELYIKQGHLQVAAGILK
                     EIIRREPQNLQAVAKLDTVRSAIALKSSAGGKFAESGDPVKTLISWLENIDGLKKHAA
                     EK"
     gene            complement(3090..>3398)
                     /locus_tag="VF305_02150"
     CDS             complement(3090..>3398)
                     /locus_tag="VF305_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7415968.1"
                     /translation="RSALAEYSKALNREPLTTLRPQIEKNIGLIYFEQNNIPEAIRHF
                     KKSLELNSSLFDAQFLLGECYLKSKDNKNAKQAFISVVKLSPQSSFGQRAKTYLQSLK
                     "
BASE COUNT          985 a          746 c          581 g         1086 t
ORIGIN      
        1 ttctatattc caacgagcat tactgtattt ttccattagt ttatttttca gagtttcttc
       61 cgtcagtgtt agagattctt ccaccaattc tatacccagt tcattaataa atccttcttt
      121 tagtttaaaa caaacttctt gcacatcaac cggcgcaaga agctcttcgt tgagcgccgc
      181 aactgatttt tttagttttt gcaattgctc aggtgtacga gtgggcagca acaacgcagc
      241 tgttttttcc gaatcaaaag acaaaagtag tgaaccctgt tgcaaaaatc cactttttct
      301 tctcacttgc gcgctgccgc aaatcttacg tcctccgact aaaagctcat ttttagaaga
      361 aaccgaaaaa cagtaggagt ttaattttcc ctttgtcaaa tccctgccat tttcatccaa
      421 atttgctttt atacctaaat aagccaagcc ttgagcaatg catttactaa taaccttata
      481 gctccctaaa atatccggtg gaaataattt ctcctcatct ctagctataa cagaatacgt
      541 aatttcctca aaatgaaaaa cggctttccc ccctgtttgc cttctgacaa tgtcaatacc
      601 catattattg catttttcaa tatttacttc tttccttaag tcttggaaat aaccaatgga
      661 aactgccgct ggatgtgagc tataaaatct aatcgttgga ggttttttat tttttatggt
      721 ctcctgaaaa atagcctcat ctatggccat attttcaaaa atgttataga atttattatt
      781 taataacctc cataccataa aaattctagt cagtatcttc ttgctggatt ataaaatcat
      841 ggtaactgga agcaacgagg agatccgcca attgttccaa gtcgtccatt tccaggacaa
      901 ttaaccaccc cgtatcatgt ggatcgctat tgattatgcc gatatcatag gtaatatctt
      961 cattaacatt aacaacggta ccattaaggg gtgaaatcaa atccactaca gctttggtag
     1021 cttcgatgga accgaatgtt tcatcgcgtt cgacataagt atcaatttcg ggaaattcaa
     1081 cagataggat ttctcctaaa ctttcttggg cataatcagt aatccctatt gtggccatat
     1141 atccatcaat cctcacccat atgtgttcac aactatagag tagatcttct ggaaatactt
     1201 tcataacagc gcaacccctg tttttattct ataataataa gttctttatc catttttgta
     1261 agcttttcgc aactattttt tttcactagt acagtatcct cgattctaat accccaaagc
     1321 cccggaatat atagtccggg ctcaatggta accaccattt gcggctttaa aatatcattg
     1381 gaattggagg caagacgagg agcttcatga acttccaaac caacaccgtg tcctgtcgca
     1441 tgagaaaaat accggccata tttttttcca aaaatgctcc gtgcacaatt gtcagtatcg
     1501 cttgcagaaa tatctgcctt aataaaatct agagttcgat catgagcatg tttcactatt
     1561 tgataaacat tcttttgctt atctgtcaac ttccccaaag caatggtgca agtttcgtcg
     1621 gaacaatatc ccccgtactt cacgccaaaa tcaataacaa taaaatctcc tttttttatt
     1681 tttctatcag tcggtttagc gtggggaagg gcagaatttt ctcccgccgc aaaaattgtt
     1741 tcgaaagcaa tttgatcggc accggactgg agagcattta actccaattg cagcgccaat
     1801 tctttttctg tacaaccagg tttaatttca gcaaggagtg acagaatagc tgctgaagaa
     1861 atatccgcag cttttttcat taataagatc tcggtttcat ctttataggc tctgagcagt
     1921 ttcagctcat cactcaaagg cacgagcact ttattccgga gacgtttggt taattggtta
     1981 tacgtttcta ccgttgtaga aaatgcttcg aaaccgatac gctttaaatt tagctccttt
     2041 acggcctgaa caattccttc aatcttatcg ttatattcta tgacttggac atttgttact
     2101 tccaatttgg cctgagttgt atatcgaccg tcaacaagca aaattgcatt accggagctt
     2161 aataaaaaag caccgtcact gccggtaaac ccggatagat aacggatgtt actcatgtta
     2221 agaaaaaata aaccatcgat tttgtaggcg ggaaaattct gctgaagtct ggatattcta
     2281 agcggtaagc tcttattttt ctgcggcatg cttctttaac ccgtcgatat tttccagcca
     2341 ggatatcaag gtctttaccg gatcgccgga ttcggcaaac ttcccccctg cggacgattt
     2401 taaagcaatt gctgatctga ccgtatcgag cttggccaca gcttgaagat tctgcggttc
     2461 tctccttatt atttccttga gaattcccgc agccacctgt aaatgacctt gtttaatata
     2521 aagctccgct aaagtaactg taaaaaaatc cggcttgagg gtaccgttat catctggcat
     2581 atcaacttca gttacaggat ggtcttcttg ttcaagaaga gccatttttt caataacttc
     2641 tctcgccttc tcagcgtttg gattcaaggc aataaacttc tcgtaacaaa tagccgcatc
     2701 ttgatgaaat cctttttccc ggtaaatatc acccatccgg tcataaataa gagacaaccc
     2761 cgaaataatt tcctccactt cccgcaaaat atcccgtgct tcattaaccc gtcccatccc
     2821 aataagggcg ttgccagcaa ttacatgggc atcgacatca acaggcaaag actgcagccg
     2881 ttcctctgct aggttaagcg cttggggaag catattctgc tgtaacagct tttctgcctc
     2941 atcaagaaaa ttcatacggg cttcttcgaa attattgctt tccatatttt gagggctaaa
     3001 caattaaaat caaaaggttt agttgattaa acgctaacac ggtgccgatt tcgtgtcaag
     3061 actggaaaga atttaatatt atggatacgt tattttaagg attgcaaata agttttggct
     3121 ctttggccga aagaagattg tggcgataac ttaacaacag agatgaatgc ttgtttggca
     3181 tttttattgt ctttactctt taaataacat tcacccaaca aaaactgagc gtcaaaaagt
     3241 gaagaattta attccaaaga tttcttgaaa tgtcgaatag cctccggaat gttattctgc
     3301 tcgaaataaa taagtccgat atttttttca atttgcggcc gcaaagtggt caagggttct
     3361 ctgttgaggg ctttgctata ttcagctaaa gcacttct
//
LOCUS       DASVIM010000108         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1492208, whole genome shotgun sequence.
ACCESSION   DASVIM010000108 DASVIM010000000
VERSION     DASVIM010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492208"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VF305_02155"
     CDS             <1..342
                     /locus_tag="VF305_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEX7415969.1"
                     /translation="KRVRDYAEVKADGTIDGKIAREALDAFEVDQKGFDQMDRKLLLT
                     LIEKFNGGPVGVENLAAAIGEEKITIEDVYEPYLIQEGYLQRTARGRIACARAYEHFG
                     LKATAGQKELF"
     gene            545..1111
                     /locus_tag="VF305_02160"
     CDS             545..1111
                     /locus_tag="VF305_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEX7415970.1"
                     /translation="MSPGLLWIPTLDYMKLLMHICCGPCTIYPLKELRIQGHEVTGVF
                     YNPNIHPYQEYQKRRQTLDDYADKAFLNVVWTEGYPLEEFLRGIAFKENERCAYCLVD
                     RLKYTAQLAIREKYDGYTTTLLYSKYQKHDLIREIGENLSQQLGIFFYYQDFRTGWAE
                     GVKISKELGMYRQSYCGCIYSEKERFYK"
     gene            1328..>1859
                     /locus_tag="VF305_02165"
     CDS             1328..>1859
                     /locus_tag="VF305_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX7415971.1"
                     /translation="MYLQRTLKKEISCSSIGLHTGRKVNMKIKPAPVDTGVIFIRKDL
                     SNAEPIHADFNKVSDTTLATSLGSNGVTVATVEHILSAFSGMGVDNAIIELDSFEVPI
                     MDGSSLPFVNLLKKVGTKVQEKNKKLLVIKKQVSVKEGDGSAVFLPAEEFKITYEIDF
                     QHAAIGKQSYSMTFSDE"
BASE COUNT          576 a          333 c          421 g          529 t
ORIGIN      
        1 aaacgtgttc gcgactatgc cgaagttaaa gcagatggaa caattgatgg gaaaatcgcg
       61 cgggaggcac tggatgcttt tgaagttgac cagaaaggtt ttgatcagat ggaccgtaaa
      121 ctactcttga cgctgattga aaaatttaac ggtggccctg ttggcgtgga gaaccttgcc
      181 gcggcaattg gcgaggaaaa aataaccatt gaagatgttt atgagccgta tttaatccag
      241 gaaggttatt tgcaaagaac ggcacgggga agaatcgctt gcgctagagc atatgagcac
      301 tttggcctta aggccaccgc aggacagaag gaactttttt aatatcaagt tgctgtcgtt
      361 aaggcgactt ttatttgtga gattcaaatt tcaaaagcga aatgcataac ctaaaggtca
      421 cacgtaacct aataccctaa ccacctaaag gtggcgcgag gtcacacgtt gaaatttgta
      481 agtcgagtcc gcctgcggcg gacctaaagt ggagggtata ataccccacc cctgggggtg
      541 gatcttgagt ccaggcttgc tctggattcc aacgcttgat tatatgaagc tattgatgca
      601 catctgctgc gggccctgca caatttatcc cttgaaagaa ttaagaattc aagggcatga
      661 agtcacaggt gttttttata acccgaatat tcatccctat caggaatatc aaaaacgccg
      721 tcaaactcta gatgattatg cagacaaagc attcttaaac gtcgtttgga cagaaggtta
      781 tccgctggaa gaatttttgc gggggatcgc ttttaaagaa aacgaaagat gtgcatactg
      841 cttagtggat cgcttaaaat atacagccca attagcgatc cgagaaaaat acgatggata
      901 tacaactacg ttgctttaca gtaagtatca aaagcatgat cttattcggg aaatcggaga
      961 aaacttaagc cagcaattgg ggatcttttt ttattatcag gattttcgta ccggatgggc
     1021 ggaaggtgta aaaatatcca aagaactggg catgtaccgc cagtcttatt gtggctgtat
     1081 ttacagtgaa aaggaacgat tctataaata aatcttatta ctttactttg gcttatatct
     1141 tctgttaatt tactgtgtct tttccaacac aggaaaatga aagccattta ttttcatcag
     1201 tgcctatcag tttaaaatca tagtgattac aataacatgg ttttagcagg aaagattaga
     1261 aaatatggta tagtccttgc tgtaaactaa acttattaaa ttcgcacttt tattttggtt
     1321 agtgactatg tatttgcagc gtacattaaa gaaagaaata agttgttcca gcattgggtt
     1381 gcataccggt cggaaagtta atatgaagat aaaaccggcg ccggtggata caggtgtaat
     1441 ttttatccgc aaggatttgt ccaatgccga accaattcat gcggatttta ataaagtatc
     1501 cgatacaacg ttggcaactt cgctgggctc taatggtgtt actgtggcta ccgttgagca
     1561 tattctttct gccttcagcg gtatgggtgt agataatgcg attattgaat tggattcttt
     1621 tgaagttccc attatggatg gtagctcgct gccctttgtg aatctactaa agaaagtggg
     1681 aacaaaggta caagaaaaaa acaagaaact cctggtaata aaaaaacagg tttccgtaaa
     1741 ggaaggcgat ggaagtgctg tgtttctgcc tgccgaagaa tttaaaatca cctatgaaat
     1801 cgatttccag cacgcagcta ttggtaaaca atcttatagt atgacatttt cagatgaaa
//
LOCUS       DASVIM010000109         3613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1662778, whole genome shotgun sequence.
ACCESSION   DASVIM010000109 DASVIM010000000
VERSION     DASVIM010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662778"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="VF305_02170"
     CDS             complement(<1..1500)
                     /locus_tag="VF305_02170"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX7415972.1"
                     /translation="MLRITDILDKTQSYLPPEDLEMIEKAYIYSASVHQGQVRLSGEP
                     YLTHPMEVAGILADMKMDAATIISGLLHDAIEDTLTTAETVEKEFGKDVSFLVSGLTK
                     LSRISFGSQEERQAENFRKMILAMSSDIRILLVRLADRIHNMRTLQYHPEDKRIYIAR
                     ETMDVYAPLANRLGINSMKMELEDLSFQYIAPDAYNELYARVAKTNESRNLYTEEVKR
                     IICTELEKYSIKGEVEGRAKHFYGIYKKMHEQHINFDEVYDLIAFRVILDSDKEKTCY
                     EALSIVHALWKPVPGRFKDYIAMPKANNYQSLHTTVIGPYGERVEIQIRTKAMHEWAE
                     KGIAAHWRYKEGRAYKDNEENQIKKLREVMEIQQEIKDPKEFMSNLKLALFPEEVYVF
                     TPNGDVKAFPKGATPIDFAYSIHTDVGNQCIGAKVNRNIVPLKYELQNGDIVEIITQS
                     SHHPSKDWLKYVVTTRAISKIRNWINAEENKSSLALGRDLLEKEFKRHNL"
     gene            complement(1529..3256)
                     /locus_tag="VF305_02175"
     CDS             complement(1529..3256)
                     /locus_tag="VF305_02175"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX7415973.1"
                     /translation="MRYSEMHLPTGREVPSDAEVISHQLMIRAGMIRKLTSGIYSYLP
                     LGYRVIRKVEQIVREEMNKAGAQEVHLPMVQPAELWQESGRWVHYGKELLRFRDRHER
                     EYCLGPTHEEVITDLVRNDVKTYRQLPRNLYQIQTKFRDEVRPRFGVMRCREFGMKDA
                     YSFDADEAGAQISYEKMFDAYNKIFKRCGLQFRPVEADSGSIGGKYSHEFMVMAESGE
                     DAMVFCEKCSYAANLEKAEVAKPEKKNVQEKDWLPLENSHTPNVRTIEEVSSFLKVKP
                     QSIVKTLIFNADGRALAVLIRGDQEVNEIKVKNYLGAAELELADDEMIKKATGAPRGF
                     AGAVKIKTRVIADYSIMNMINFVTGANKEDCHLKNVNLGRDFDVESFADLRIVEEKDS
                     CPRCGGKIQFARGIEVGHVFKLGTKYSQAMKAVYLDKDGQEKIMIMGCYGIGIGRTVA
                     ACIEQNYDANGIIWPVPLAPFHVIITPVNVNEKDVFDAAENLYISMLAHGIEVIFDDR
                     DERAGVKFKDADLIGIPLRVVIGQKNLSQGNVELKIRKTGENKLYLLQNIVQEVKQLI
                     AQELNPGAQ"
     gene            complement(3256..>3613)
                     /locus_tag="VF305_02180"
     CDS             complement(3256..>3613)
                     /locus_tag="VF305_02180"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX7415974.1"
                     /translation="VHEIPLAYSILRALKIRMVGPEIVSCPTCGRCQIDLFTLTQKIE
                     NALSGRKDYLKVALMGCVVNGPGEAADADIGIAGGHGFGMLFKRGKATKKIAEKNFVP
                     VLLEEINSLLKGKKEV"
BASE COUNT          934 a          900 c          674 g         1105 t
ORIGIN      
        1 taaattatgt cttttaaatt ctttttccag cagatcacgc cccaaagcta agctgctttt
       61 attttcttct gcgttaatcc aatttctgat tttggaaatt gctcttgtag ttactacata
      121 tttcaaccaa tccttactgg ggtgatgact ggattgcgtt atgatttcaa caatatcgcc
      181 attttgcaat tcatatttta atggaacaat atttctattg acttttgctc caatgcattg
      241 attaccgaca tcggtatgaa tactgtaagc aaaatcaatg ggagtagccc ctttgggaaa
      301 agctttgaca tcgccattgg gtgtaaaaac atagacttcc tccggaaata gcgccaattt
      361 caaattggac ataaattcct tcggatcttt tatctcttgc tggatttcca tcacttctct
      421 gagtttttta atctgatttt cttcattgtc cttgtaggca cgcccttcct tataacgcca
      481 atgagcagca atgccttttt ccgcccactc atgcattgct ttagtcctga tctggatttc
      541 caccctttct ccataaggcc cgatgaccgt agtgtgcagc gactgataat tattggcctt
      601 gggcatagcg atgtaatctt tgaatctgcc gggaaccggc ttccacaaag catgcacaat
      661 actcaacgcc tcatagcaag ttttttcttt gtcggaatca agaatcacac ggaatgcaat
      721 gaggtcgtat acttcatcaa aatttatgtg ctgttcgtgc atctttttgt aaatgccgta
      781 aaaatgcttt gccctcccct caacttcgcc tttaatggaa tacttttcca actccgtgca
      841 aattattctt ttgacttctt ctgtataaag gttgcgtgat tcatttgttt tagcgacgcg
      901 tgcatataat tcgttatatg catcgggagc aatatactga aaagacaagt cctctaattc
      961 catcttcatc gagttaattc caagacgatt ggccagaggc gcatatacat ccatagtttc
     1021 tctagcaatg taaattcttt tatcctccgg gtgatactgt aaagtcctca tgttgtgaat
     1081 acggtcagcc aggcgtacca gcaaaatgcg gatatcggaa gacatggcta aaatcatctt
     1141 gcggaaattt tccgcctgtc tttcctcttg agaaccaaaa gaaatacggc tgagcttcgt
     1201 caatccgctt actaaaaaag atacgtcttt gccaaattcc ttctcgactg tttccgctgt
     1261 agttaatgta tcttcaattg catcgtgcaa caatccgctg attatcgtcg cggcatccat
     1321 tttcatatca gccaggatgc cggcaacttc catgggatgg gtaagatatg gttcaccgga
     1381 taaccttacc tgaccctgat gaactgacgc cgaatagata taagctttct cgatcatctc
     1441 caagtcttct ggaggtaagt acgattgggt tttatcgaga atatcagtga tacgtaacat
     1501 taaagttccc agctaatttg acttgcggct attgtgcgcc cggatttaat tcttgcgcta
     1561 tcagttgttt aacttcttgt actatattct gtagcaaata aagcttgttt tcgccggtct
     1621 ttctaatctt cagttcaaca tttccctggg aaagattttt ctgtccgata actactcgga
     1681 gaggaattcc gattaaatcg gcatccttga acttcacacc ggctctttca tcccggtcat
     1741 cgaaaataac ctcaattccg tgagcgagca ttgaaatata aagattttcc gcggcatcga
     1801 agacatcctt ttcgtttaca ttcaccggag ttataatcac atggaatggc gccagaggca
     1861 caggccagat gattccattg gcatcgtaat tttgttcaat gcaggcggca actgttctgc
     1921 caatgccaat tccgtagcaa cccatgatca tgattttttc ctgaccgtct ttatccaaat
     1981 acactgcctt catcgcctga ctgtattttg tacccagctt aaagacatgg cctacttcaa
     2041 ttccccgcgc aaattgaatt ttaccgccgc agcggggaca actatccttt tcttcgacaa
     2101 tacgtaaatc ggcaaaagat tcaacatcaa aatcccggcc gaggttaaca ttcttcaaat
     2161 ggcaatcttc cttattggcg ccggtgacaa aattaatcat gttcattatg gaataatcgg
     2221 caattactct cgttttaatt ttgacggctc cggcaaatcc ccgcggcgcg cccgtcgctt
     2281 ttttaatcat ttcatcatcg gccagttcca gctcggctgc accaagatag tttttgacct
     2341 ttatttcatt tacttcctga tcgccacgaa tgagcacagc taaagcccgg ccatcagcat
     2401 taaagatcaa tgttttaaca atactttgag gttttacctt caaaaaagag ctgacttctt
     2461 cgattgttcg cacgtttggt gtgtgactat tttccagagg cagccagtcc ttctcttgga
     2521 cattcttttt ttcaggcttg gcgacttcag ctttttccag attggccgcg taggaacatt
     2581 tttcgcaaaa taccatggcg tcctcaccgg attcggccat aaccataaat tcatgagaat
     2641 atttgccgcc aatgctgccg gaatcagcct ctaccggacg aaattgcaat ccacaacgct
     2701 tgaagatttt attataagca tcaaacatct tttcgtaact tatttgggca cccgcttcat
     2761 cggcgtcaaa actgtaggcg tctttcatgc cgaattcccg gcaacgcatc acaccaaagc
     2821 gcggacgcac ttcatcgcgg aattttgtct gaatttgata aagattgcgg ggcaattgac
     2881 ggtaggtctt gacgtcattg cggactaaat ctgtaataac ttcttcatga gtcggcccca
     2941 gacagtactc ccgttcatga cgatcgcgaa atcttaaaag ctccttccca taatgtaccc
     3001 acctgccgga ttcctgccaa agctccgccg gctgcaccat aggcaaatga acctcctgcg
     3061 ctccggcctt attcatttct tcccgcacaa tttgttctac cttgcgaata acgcgatagc
     3121 ccaaaggcaa atatgaatat ataccgctgg ttaatttcct gatcatcccc gccctaatca
     3181 tcagctggtg actgataacc tcagcatcgg agggaacttc ccttcccgtg ggcaggtgca
     3241 tttccgaata acgcattaaa cctccttttt ccccttaagc agcgaattta tctcttccaa
     3301 taaaaccggc acaaagtttt tttcggcgat tttttttgtt gctttacccc ttttaaaaag
     3361 catgccgaac ccatggcctc cggcaatgcc gatatccgca tcggcagcct cacccggacc
     3421 attgacaaca cagcccatca aggctacttt caaatagtct tttctgccgc taagcgcgtt
     3481 ttctatcttt tgagtcaacg taaaaagatc aatttgacaa cgtccgcaag taggacagga
     3541 aacaatctcc ggcccgacca ttctaatttt caacgcacgc aaaatgctgt aggccagagg
     3601 aatctcgtga aca
//
LOCUS       DASVIM010000110         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1579135, whole genome shotgun sequence.
ACCESSION   DASVIM010000110 DASVIM010000000
VERSION     DASVIM010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579135"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VF305_02185"
     CDS             complement(<1..207)
                     /locus_tag="VF305_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX7415975.1"
                     /translation="MSKTSDKVKVGLATLGCKVNQYESAVLAEDLHNKDFSLVPFNSR
                     ADVYIINTCTITAFSDFQARQCIRR"
     gene            429..779
                     /locus_tag="VF305_02190"
     CDS             429..779
                     /locus_tag="VF305_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415976.1"
                     /translation="MDAIAGYIDAHPAVLIMIVIFVVILILYFIFKKFIKFVLLLLFI
                     LLAAGGYYYFKDPAKMPEKIKKSVDMMKSGINEVADKSKSFYRDTKELYKKSKEVPGD
                     LNKLLKDSNEKAGK"
     gene            842..1756
                     /locus_tag="VF305_02195"
     CDS             842..1756
                     /locus_tag="VF305_02195"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX7415977.1"
                     /translation="MSNSSKTISWRFFPLLLILIFLVIAFLRTPKPCQEPLTYRIGKV
                     DERFDLSRQEFGVTVGMAAAMWGKPLFRELFREDPNGAIEVNLIYDYRQEASDRLKKL
                     NYKIDRSKNYYEELKLRLENLKVEYEQKNAALANDLNFYNSRVNAFNIEIQSWNRRGG
                     VPESIHGRMMKEKDELNSLLENLHVRQEEIKKLADTINSMVVVINEIASDYNLDLVDH
                     QNVGSTLGKEFGEGLYEYKNGKRSITIYQFDNGYRLVRVLAHEFGHVLGLNHSKSDEA
                     VMYHLIQSDSLVLTADDIAALKGHCKIQ"
     gene            complement(1816..2298)
                     /locus_tag="VF305_02200"
     CDS             complement(1816..2298)
                     /locus_tag="VF305_02200"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7415978.1"
                     /translation="MEKTLVFIHGLEGTAQGTKGQFFHQNFPQMIIEDYIGAFALRMR
                     KLASALTGKTDLIIVGSSYGGLMAVQYARKNEIRVKKLILLAPALNLPEFSHNTHKQL
                     QLPVIIYHGIDDNIVDPYIVKNIALKSFCTLEHHLVEDDHSLHNTFLVLDWRKLLEID
                     "
     gene            2403..>3015
                     /locus_tag="VF305_02205"
     CDS             2403..>3015
                     /locus_tag="VF305_02205"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX7415979.1"
                     /translation="MALISPFKAVRPQRQFVSQVAALPYDVMTREEAQKAVAGNALSF
                     LHVEKSEIDVPDGTPNDDDLIYQTAKRNFAQMREKGILLQDKSPCFYIYRQQMGARVQ
                     TGIVGLVSAREYDAGKIKKHELTRQDKEEDRTRHVNAVNAQTGPVFITYRYRAKLDEI
                     INQALTGNPEYNFTADDGVIHKVWVVDNPKQIQDIKNEFLSVDA"
BASE COUNT          960 a          527 c          642 g          886 t
ORIGIN      
        1 ccggcggata cactggcggg cctgaaaatc ggaaaatgcc gtgatcgtac aggtgttgat
       61 aatataaaca tcagccctag aattgaaggg gaccagagaa aaatctttat tatgtaaatc
      121 ttcagctaag actgccgatt cgtattgatt gactttgcat cctagagtgg ccagtcccac
      181 ttttacttta tccgatgtct tgctcaattc atcccccgac aaaaataaat aatctttggc
      241 taatattatt aatcttataa ataattttgc ctttgataaa aacctgaacg gctgggaaaa
      301 gttcaccgat aatttgctga aatctagtca gtagggcaat gtttgtcaag attaaataat
      361 aatggatttc ataataaaat taagataaat tgaaccaaaa aaagagggaa aatagggagg
      421 tctttataat ggatgcaatt gctggttata tagacgcgca cccggcagtg cttataatga
      481 ttgttatttt tgtcgtaata ttaattctgt attttatttt taaaaagttt atcaaattcg
      541 tattgttatt gttattcatt ttgttggctg cgggtggtta ttactatttt aaagatccag
      601 ctaaaatgcc tgaaaaaatt aaaaaatcag ttgatatgat gaagtccggc atcaatgaag
      661 tggcggacaa aagcaagagc ttttataggg acaccaagga actatacaaa aaaagcaaag
      721 aagttcccgg tgatcttaat aaattgctca aagactcgaa tgaaaaagcg ggaaagtgat
      781 gctgataaat tggtaaagag tcgtagattc gtcaatattc tgattttccc aactctctga
      841 tatgtcgaat tcgtctaaaa caattagctg gcgattcttt cctttgctgc tcatcttaat
      901 ttttctggtt atagcttttc ttcggacgcc taaaccatgt caagagcctt taacataccg
      961 tataggcaag gtagatgaaa gatttgattt aagtcgtcaa gaatttggcg tgacagtggg
     1021 tatggctgct gcaatgtggg gaaaaccact tttccgcgag ctttttcggg aggatcccaa
     1081 cggcgcaatt gaggttaatc ttatttatga ctaccggcaa gaagcctcag atagactcaa
     1141 aaaactcaat tataaaattg atcgctccaa gaattactat gaagaattaa aattacgtct
     1201 ggaaaattta aaagtggaat acgaacagaa aaatgcagcg ttagctaacg atttaaattt
     1261 ttacaactca cgggtaaacg cctttaatat cgaaattcaa tcatggaaca ggcgcggtgg
     1321 agttcctgaa agtatccatg ggcggatgat gaaagaaaaa gatgaattaa attctttgct
     1381 ggaaaatctc catgtccggc aggaggaaat aaaaaaactg gcagacacta tcaacagcat
     1441 ggtagttgtc atcaatgaaa ttgcctctga ctataatctg gatttagttg accatcaaaa
     1501 cgtgggaagc accttgggaa aagagttcgg tgaaggatta tatgaataca agaacggtaa
     1561 acgatcaatt accatttatc agtttgataa cggctacagg ctggtgcggg tgcttgccca
     1621 tgaattcggt cacgtccttg gcttaaacca tagtaaaagc gacgaggctg ttatgtacca
     1681 tttaattcaa tctgattcgc tggtgcttac ggcggatgat atagcggcgc tgaaagggca
     1741 ttgtaaaatt caatgaggcc agttttataa aaaagtttcc ggcgttctat tgtaattatg
     1801 aaatatttct aaccattaat ctatctccag aagcttccgc caatcgagaa ctaggaaagt
     1861 attatgtaag gaatggtcat cctctaccag atgatgttcc agagtacaaa aagattttaa
     1921 ggcaatattt tttacgatgt aaggatcgac aatgttgtcg tcaattccat gatatataat
     1981 aaccgggagc tgcagttgtt tatgggtgtt gtgactaaat tcgggaagat tcaaggccgg
     2041 agcaagtaga atcaattttt ttactcggat ttcgtttttc ctggcgtatt gcacagccat
     2101 taaacctcca tagctcgatc ctactatgat caggtcagtt tttccggtaa gggcactggc
     2161 cagtttacgc atgcgcaggg caaaagctcc aatataatct tcaataatca tctgtggaaa
     2221 attttgatga aaaaattgtc ctttagtgcc ttgcgccgtg ccttccaaac catgaataaa
     2281 aaccaatgtt ttttccatat gcctttttcc ccggatattt tttacaaaaa aatattttta
     2341 ttaattttgc gttgcctgaa ttatgacagt atgttatcag tcccgcaaga ggagatttga
     2401 ttatggcgtt aatttcacca tttaaggctg ttcgtccgca gcgtcaattt gtatcacaag
     2461 tagcggcgct accctatgat gttatgacca gagaagaggc tcaaaaggca gttgccggca
     2521 atgctttgag ttttctgcat gtagaaaaat cggaaattga tgtgcccgat ggcaccccaa
     2581 acgatgacga cctgatttat cagacagcta aacgtaactt tgctcaaatg cgggaaaagg
     2641 ggatattgct gcaagataaa tcgccgtgct tttatatcta tcggcagcaa atgggtgctc
     2701 gggtgcaaac tggaattgtt ggattagtga gcgccaggga gtatgatgcc gggaaaatca
     2761 agaagcatga actgacaagg caagacaaag aagaagatcg tacccgtcat gtgaatgctg
     2821 ttaatgcgca aacaggtccg gtatttatta cttaccgtta tcgagctaaa ctggatgaaa
     2881 ttataaatca agctctgacc ggtaatcctg aatataattt tactgcggat gatggtgtaa
     2941 ttcacaaagt atgggttgtg gataatccaa aacagattca agatatcaaa aatgaatttt
     3001 tatcagttga tgccc
//
LOCUS       DASVIM010000111         3604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_554203, whole genome shotgun sequence.
ACCESSION   DASVIM010000111 DASVIM010000000
VERSION     DASVIM010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554203"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1055)
                     /gene="cydB"
                     /locus_tag="VF305_02210"
     CDS             complement(48..1055)
                     /gene="cydB"
                     /locus_tag="VF305_02210"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX7415980.1"
                     /translation="MENIQNFQILWFILIGILFLGYSLLDGFDLGIGTLLPFLGKKKE
                     EKDILINSIGPVWDGNEVWLITGGGALFAAFPQAYATALSGFYPAILLVLLALIFRAV
                     SQEFRANDEARTGIWEKAFVMGSALAALLFGVVLGDVIYGVPLDNKMEFTGNFFTLLR
                     PVALLFGITGFAAVLLQGASYAALKTEGELQQRSFRAVNILLWVNVIAASSYFTVMTF
                     TFQQLVVNPLFYVAVLLTALGLTGIFFSIKKKNDKAPFWESSFSFIGLWLAAGAAQFP
                     NLIKATNAPDLSITIFNSSTSLQTLKLMSVIALIGMPIVIGYTIFVYRIFKGKTKADH
                     Y"
     gene            complement(1097..2404)
                     /locus_tag="VF305_02215"
     CDS             complement(1097..2404)
                     /locus_tag="VF305_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX7415981.1"
                     /translation="MSALLLARIQFAFTVGFHFLFPAMTLGTALIILITETLYLIKKD
                     EIYKKITDFLAKLLGLIFVIGAATGITMEFSFGMNWSGYSRAVGDIFGPILAAEGIIA
                     FFLESVFIGVLIFARNKVSPRVYWLAVLLVFLGGHLSAFWIVVANSWMQTPAGYQINE
                     AGKIILSNFTQAVFNPSTMIRFYHTVMASWMTCAIMIAGIAGYYVRKGLHGQTARTML
                     KIGIILFAITPLLQLATGHVHAIEVINMQPEKAAAYEGHFVTKKGVPIYIAGFVDEQN
                     ERTYGIYVPNSLSFLYNFDWNSEIKGLKEFPKENWPPVNFVFQTYHIMVGIGFFSIAM
                     GLLGVFLLVTKKIYEAKFYLFILPFLMPLPHIAHETGWIAAEVGRQPWIIYKLMKTAN
                     AASVVVSASNIIFSLLMFFLIYLLLFIMFILLFLKFVKKGPAL"
     gene            2542..3102
                     /locus_tag="VF305_02220"
     CDS             2542..3102
                     /locus_tag="VF305_02220"
                     /inference="COORDINATES: protein motif:HMM:NF045043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415982.1"
                     /translation="MANLIRCKACSYITEEGKIKDVCPACGVPAKMFEPYTDPLSEKR
                     RRILDLHIHPIIVHFPQAFAFTLFALAILFFIAPSPIQNAFYYTMQVISFFLPLVVVL
                     SFLTGLFDGKTRFRKVTTPFLKRKIIIGLIFFITSIALVVVAFTQQLSAISVMVLFTF
                     LTIMAVVCAVSLGLIGGRLVDAKFPG"
     gene            3214..3588
                     /locus_tag="VF305_02225"
     CDS             3214..3588
                     /locus_tag="VF305_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415983.1"
                     /translation="MKRTIGISTFIFGALLVFSMVYCVYSPLAYANQPQDVKLAYDSN
                     AQTLTVTITHKSSFTSFHYVKSVEIEKNGIGIITNTYHSQPDPATFAYNYKISAGKGD
                     TLEVTANCSLSGSKTATLEVGK"
BASE COUNT         1099 a          773 c          697 g         1035 t
ORIGIN      
        1 cataatctcg attcgtcctt acactaaaga tgacacgtcc aaattactta atagtgatca
       61 gccttggttt tacccttaaa aatcctgtaa acaaatattg tataaccaat tactatcggc
      121 attccgataa gagcgatgac actcataagc tttagcgttt gtaaactggt ggagctatta
      181 aaaatagtta tgcttaaatc aggggcattg gttgctttta ttagattagg gaattgcgcg
      241 gctccggcag caagccagag gccgataaac gaaaatgatg attcccagaa aggggctttg
      301 tcatttttct tttttatcga aaagaatatt cccgttagcc ccagcgctgt gagtaatacc
      361 gccacataga ataatggatt tacgacgagc tgctgaaaag taaaagtcat cacagtaaaa
      421 taactgcttg cggctatcac gttgacccac agcaaaatat taactgcgcg aaatgacctc
      481 tgctgtaatt cgccctcagt tttcagcgcc gcataagaag ccccctgcag cagaacagcg
      541 gcaaatccgg tgatgccgaa taaaagcgcc acagggcgca gcagtgtaaa aaaattgcca
      601 gtaaattcca ttttattatc cagcggcact ccatagataa cgtcacccag aactacgcca
      661 aataacagag ccgcaagagc actgcccata acaaaagctt tctcccagat tccggttctg
      721 gcttcgtcat tagcgcggaa ttcctgcgac actgctctaa atatcagtgc cagcaaaaca
      781 agcagtatcg ccggataaaa accagataaa gccgtagcat aggcctgcgg aaaagcagca
      841 aagagagcgc caccgccggt aatcagccaa acttcattgc cgtcccatac aggtcctata
      901 gaatttatta ggatgtcctt ttcctctttt ttcttgccga gaaacggcag gagcgttccg
      961 atccccaggt caaaaccatc cagaagtgaa tagcccagaa aaagtatacc tataagaatg
     1021 aaccataata tttgaaagtt ttgaatattt tccatttcct tgctctttct ctatttttaa
     1081 ccggccattt taccaattat aatgccggac ctttttttac aaattttaag aacaacagga
     1141 taaacataat aaacagcagc aaataaatca ggaaaaacat aagcaaacta aaaatgatat
     1201 tactcgcaga cacaaccact gaagcggcat tcgcggtctt catcagctta taaattatcc
     1261 atggttgtcg gcctacctcc gcggctatcc agcctgtttc gtgggctata tgtggtaacg
     1321 gcatcaagaa aggaagaata aagagataga atttggcctc atagattttt ttggttacaa
     1381 gcaaaaaaac tcccagcaaa cccatggcaa tagaaaaaaa tcctatcccc accattatat
     1441 gataagtttg aaagacaaaa ttgacgggcg gccagttttc tttggggaat tctttcaatc
     1501 cttttatttc cgaattccag tcgaagttgt aaagaaaact caaagaattg ggtacataaa
     1561 ttccataggt tctttcgttt tgttcatcga caaatcccgc gatataaata ggtacgcctt
     1621 ttttcgtcac gaaatgtcct tcgtacgcgg ccgccttttc gggctgcata ttgattacct
     1681 ctatcgcatg cacatggcct gtggccagct gtaaaagcgg agtaatggcg aaaagtatta
     1741 tgccgatttt cagcatcgtc ctggctgttt ggccgtgcag accttttctt acgtaatatc
     1801 cggcaatgcc cgcgatcatt attgcacagg tcatccacga agccattacc gtgtgataaa
     1861 aacggatcat tgttgacgga ttaaaaactg cttgtgtaaa atttgacaaa ataattttcc
     1921 cagcttcatt tatctggtac ccagccggag tttgcatcca ggaattggct accactatcc
     1981 agaaagcgga aagatgtccg cctaaaaata ctaacagcac ggccagccag taaactcttg
     2041 gagaaacttt gtttctggca aaaattaaaa cgccgataaa tactgattcg agaaaaaaag
     2101 cgataatgcc ttcagcagcc agaattggtc cgaagatatc tcctactgct ctggaatagc
     2161 ccgaccagtt cattccaaag gaaaactcca tagtaatgcc tgttgccgcg ccgattacaa
     2221 atatcaatcc gagcagttta gccaggaaat cggtgatctt tttataaatt tcatctttct
     2281 tgatcagata taatgtttcg gttataagta tgatcagggc agtgcccaac gtcatagccg
     2341 gaaaaagaaa atggaagcct acagtaaagg cgaattgaat ccgggcaagg agtaatgcgc
     2401 tcatagacac cttcctatat ttgattgcgt aacattatcg gataaatttc tacttttcaa
     2461 taatataatt ttttaattgt tatttgataa tgaaacatat tattgtataa tctaacaaaa
     2521 attaaatcgt caggagatat tatggcaaac ttgatcaggt gcaaagcgtg cagttatata
     2581 accgaagaag gtaagataaa agatgtatgc ccggcatgcg gagtgccggc gaaaatgttt
     2641 gaaccttata cagacccttt atcggaaaag agacgcagaa ttcttgatct tcatattcat
     2701 cccattattg tgcattttcc tcaagctttc gccttcacac tttttgcctt ggcgatttta
     2761 ttttttattg ccccatctcc tattcaaaat gcattttatt acactatgca ggtgatatca
     2821 tttttcctgc cccttgtcgt tgttttatct tttttaaccg gactatttga cggcaaaacc
     2881 agattccgga aagtgacaac gccttttttg aaaaggaaaa ttattatcgg cttgatcttc
     2941 tttatcacat ccatcgccct tgttgtcgtt gcgtttacac aacaactctc agctatttct
     3001 gtgatggtat tattcacttt tttgacaata atggctgtgg tctgcgctgt ttcgctgggc
     3061 ttgattggtg gcagactggt ggatgcaaaa tttcccggct gaggctttca gtttttaatc
     3121 gctgttttgc cgccattgct tttgtcaatc ttttgcgtta ttgattaacc ttaatatggt
     3181 taaaagaaat catagacgaa aaggagattt aaaatgaaaa gaacaattgg gatttcaact
     3241 tttatttttg gtgcgttgtt ggttttttca atggtttatt gtgtttattc gccgcttgct
     3301 tatgccaatc agccgcagga tgtaaaactt gcctacgatt cgaatgcaca aactttaact
     3361 gtaacaatta cccacaaatc atcattcacc agttttcact atgttaaatc cgtggaaatt
     3421 gagaaaaacg ggattgggat catcacaaat acatatcact cacaacccga tccggcaaca
     3481 tttgcgtata actataagat atctgctggc aagggcgata cactggaggt aactgccaac
     3541 tgtagtcttt cgggaagcaa aacagcaaca cttgaggttg gaaaataaaa atcgctaagc
     3601 gggc
//
LOCUS       DASVIM010000112         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_641001, whole genome shotgun sequence.
ACCESSION   DASVIM010000112 DASVIM010000000
VERSION     DASVIM010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641001"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VF305_02230"
     CDS             complement(<1..296)
                     /locus_tag="VF305_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415984.1"
                     /translation="MSEEKVYIKNGSLIIEGLLSKTSGEKGVVICHPHPLMGGSMYNN
                     VVAAIQGAFAAVNYSTLRFNFRSAGKSTGVYDEGIGEKEDIIAAGKYLEKLGVK"
     gene            complement(343..1311)
                     /locus_tag="VF305_02235"
     CDS             complement(343..1311)
                     /locus_tag="VF305_02235"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX7415985.1"
                     /translation="MLEELVKIHELKKIYTLSSGMFNSQKRIVPAVNNVDLQIFKGET
                     LGLVGESGCGKSTLARLIARLEKPSSGNIYFKGEDIFLYDKKALKAYRRNVQLIFQDP
                     YSSLNPRKSAVSIIKEPLTIHRIGNKNQRQESVAELMEKVGLNKEQANRYPHEFSGGQ
                     RQRIGIARALILQPELIIADEPVSALDVSIQAQILNLLKDLKNDFGLTYLFISHDLNV
                     IRFISDRIAVMYLGKIVELAANSDIYNRPLHPYTRMLLSAVPLCDPQKKKRIMTIKGE
                     ARAEAEEGCSFQNRCPYKMEICVKIEPRLEKFDKQSLCACHRAGSI"
     gene            complement(1506..2504)
                     /locus_tag="VF305_02240"
     CDS             complement(1506..2504)
                     /locus_tag="VF305_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7415986.1"
                     /translation="MPPLLEIKNLMTVFDTARGRIKAVDGVSLGLNSGETLGIVGESG
                     CGKTMLALSIMRLIPANGKIINGEIFFLGQDLLKKSDEEMRALRGSDVAMIFQEPMTS
                     LNPVFRVGEQIAEAIRLHQQLPAKEALALSVEQLREVGIPDPEKRAQDYPHQLSGGMR
                     QRVMIAMAMSCHPKLLLADEPTTALDVTIQAQILDLISDLKKKNQMTVVLISHDLGVI
                     AQEAQKIAVMYAGKIVESATVEQIFVKPLHPYTKGLIDSLPARCVGVDKEQQKYLKTI
                     PGFVPSLYNLAPGCRFYDRCSYADNHCAQEEPLLDEIEQGHFVACWNINDRKTDKI"
     gene            complement(2508..>2973)
                     /locus_tag="VF305_02245"
     CDS             complement(2508..>2973)
                     /locus_tag="VF305_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415987.1"
                     /translation="NTDAEGRLILADALAYASKYKPEAIIDVATLTGACIVALGDDVI
                     GMLGTDDKLKNEINKAAQATGELVWELPLWESYSELIKSDIADYKNSGGRSAGTITAA
                     AFLSKFVGDSPWVHLDIAGPAWSNKDKTYIPKGASGVTVRLLVEFLRNRAGR"
BASE COUNT          784 a          722 c          612 g          855 t
ORIGIN      
        1 tttttcaccc caagtttttc caggtatttg ccggccgcaa taatatcttc cttttcgcca
       61 attccttcat cgtaaacacc ggtgctctta ccggcacttc taaagttaaa tcttaaagtc
      121 gaataattta cggcagcaaa cgcaccctgt atagctgcaa cgacattgtt atacattgaa
      181 ccacccatta agggatgtgg atggcaaatc acaacacctt tttctcctga agttttgctt
      241 aacaatcctt cgattatcaa tgaaccgttt tttatatata ccttttcttc cgacataatc
      301 actcccagac caagttgcat tcaacaggct ccttcgtatc aactaaattg atccggccct
      361 gtggcaagca caaagacttt gtttgtcaaa tttttccagc cgcggttcta tcttcacaca
      421 aatttccatt ttataaggac agcggttttg aaaagagcac ccttcctcag cttcggccct
      481 agcttctcct tttattgtca ttattctttt tttcttttgg ggatcgcaga gtggcactgc
      541 cgaaagcaac atgcgagtat aaggatgaag gggacgatta taaatatcgc tgtttgctgc
      601 cagttcgaca attttaccca agtacattac cgcaatgcga tcggaaatga atcgaataac
      661 attaagatca tgagaaataa ataagtaggt caatccgaaa tcattcttta aatctttgag
      721 caaattaaga atttgcgcct gaatcgatac atccaaagcg gaaaccggct catcggcaat
      781 aatcaattcg ggctggagaa taagcgcccg cgcgattcca atacgctgtc tctgtccgcc
      841 gctgaactca tgcggataac gatttgcttg ctctttgttc agaccgactt tctccatcaa
      901 ttcggccaca gattcctggc gctgattttt attgccgatt cgatgtatgg ttaaaggttc
      961 tttgattatg cttactgccg atttacgggg gttgagagag gaatacggat cctgaaagat
     1021 taattgcacg ttgcggcgat atgcctttaa ggcttttttg tcatataaaa aaatatcttc
     1081 gcctttaaaa taaatattac cggaggaagg tttttctaat ctggctatca ggcgcgccag
     1141 ggtagatttt ccgcaaccgg attcaccgac caaacccaga gtttcgcctt taaaaatctg
     1201 caggtcaaca ttgttgacag caggaacaat tctcttttgg gaattaaaca tgccgctgct
     1261 taaggtatag atttttttta gttcgtgaat tttaaccaat tcttccagca ttttcgactc
     1321 acataaatat tgtcattccc acgaagaggt tgtgttacaa tcctcatttg tcattgctag
     1381 tccgcagcga cggacgtggc aatctttata ttatcatgta cttatgcgat tgcttcgttc
     1441 gcttcgctcc ctcgcaaaga cattatgaca caatctcgaa ggcgggaatg acaaatcagc
     1501 aatagttaaa ttttatccgt tttcctgtca ttaatattcc agcacgcgac aaaatgcccc
     1561 tgttcgatct catctaacag gggttcttcc tgagcgcagt ggttatccgc ataagaacac
     1621 cgatcataaa aacggcagcc gggggcaaga ttgtataaac tgggaacaaa accaggaatg
     1681 gtcttaagat atttctgctg ctctttatcc acacctacgc atcttgccgg aagcgaatcg
     1741 atcaaacctt tggtatatgg gtgcaagggc tttacgaaaa tttgctcgac agtcgcactc
     1801 tccacaattt tacctgcata cattaccgca attttttggg cctcctgagc aataacgccc
     1861 agatcatgcg aaattaagac aaccgtcatt tgatttttct tcttcagatc ggagatcaaa
     1921 tccagaattt gcgcctggat ggtaacatcg agcgccgtgg taggttcatc agcaagcaga
     1981 agctttgggt gacaagacat ggccatggca atcatcacgc gctggcgcat gccgccgctg
     2041 agctgatgcg ggtaatcttg cgcccgcttt tccggatcgg gaattcccac ttcgcgcaac
     2101 tgctccacgc ttaaggccag ggcttctttg gccggaagtt gttggtgcaa ccggatagcc
     2161 tcggcgattt gttcaccaac gcgaaagaca gggttgagcg aagtcatcgg ttcttgaaaa
     2221 atcatggcaa catcactgcc ccgcagggcg cgcatttctt cgtctgattt tttcaataaa
     2281 tcctgaccta aaaagaatat ttcgccatta atgattttgc cattggcagg gatgagccgc
     2341 ataatcgaaa gggcaagcat tgtttttccg caaccggatt cgccgacaat tcccagagtt
     2401 tcaccggaat taaggcccag cgaaactccg tccacagcct taattcttcc tcgagcagta
     2461 tcaaatactg tcattaaatt ttttatttcc aaaagcggcg gcatgttcta cctaccggca
     2521 cggttacgta aaaattccac cagcaatcgg acagtgactc ccgatgcccc tttggggata
     2581 tatgttttat ctttattact ccaggccgga ccggcgatgt ccaaatgcac ccagggtgag
     2641 tctccgacaa atttgcttaa aaatgccgcg gcggtgattg tgccggcaga ccgtccgccg
     2701 ctatttttat aatcggcaat gtcgctcttg ataagttcgg aatagctttc ccacaaaggc
     2761 agctcccata caagttcgcc agtcgcttgg gcggctttat ttatttcatt ttttaattta
     2821 tcgtcagttc ccaacatacc gataacatca tctcccaatg ctacaatgca ggcaccggta
     2881 agagtggcaa catcaatgat tgcctcaggc ttatacttgg acgcgtaagc aagcgcgtcg
     2941 gctaaaataa gccggccttc ggcatccgta ttg
//
LOCUS       DASVIM010000113         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_386028, whole genome shotgun sequence.
ACCESSION   DASVIM010000113 DASVIM010000000
VERSION     DASVIM010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386028"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VF305_02250"
     CDS             complement(<1..476)
                     /locus_tag="VF305_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HEX7415988.1"
                     /translation="MKKISTIDDLKKAGEKGLKSLFPDGVKIVVGMATSGIAAGADKV
                     YAALNEEIKKRKLSYILAKTGSMGMDFIEPLVDVIEKDKPRLSYGKMTPAKVPQLIDQ
                     IIKGNIDKIKPLYRIDEEDIIITNEKYQYLKVKVPEYIKDLPKISELPFYKKQVKIV"
     gene            complement(481..972)
                     /gene="nuoE"
                     /locus_tag="VF305_02255"
     CDS             complement(481..972)
                     /gene="nuoE"
                     /locus_tag="VF305_02255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX7415989.1"
                     /translation="MKKATTTAATTIEAKTKEIVKKHGADKSSLIAVLQDIQEQFNYL
                     PKDALATVNEEMEIPASRIYEVATFYNSFSLNPRGRYLIEVCAGTACHVQGASRLMDK
                     LKRDLEIECGETTKDNMFSLEEVRCLGCCSLAPVVRIGGEIHPYLTQDKIPKILGNYS
                     KQG"
     gene            complement(1014..>1692)
                     /locus_tag="VF305_02260"
     CDS             complement(1014..>1692)
                     /locus_tag="VF305_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7415990.1"
                     /translation="LKALRKKGRIDNRYIKVVGMAGDGGTMDIGLQALSGAMERGHDM
                     LFVCFDNEAYMNTGIQRSSGTPMGASTTTAPAGKISYGQKTWKKNMPEIMVAHNIPYV
                     ATASPSYPLDFIRKVEKAKSVKGPSYIHCFSVCPTGWRSPSHLTIALGRLVVETGIFP
                     LYEVENGKYKISAEMPKKLKPIKDYFKTQGRFRHLGEHEIKMIQDRVNSEYKKLLEKV
                     KCLKAWD"
BASE COUNT          411 a          393 c          342 g          546 t
ORIGIN      
        1 acaattttaa cttgtttttt gtaaaagggg agttcgctaa ttttaggcaa atccttgatg
       61 tattccggca ctttcacttt taagtattga tatttctcat ttgtgatgat gatatcttct
      121 tcatcaatac gataaagcgg cttaatttta tcgatattac ctttgatgat ttgatcaatg
      181 agctgcggca ctttggccgg agtcattttt ccgtaagaaa ggcgcggctt gtctttttca
      241 ataacgtcaa ccagcggttc aataaaatcc atgcccattg aaccggtttt agctaagata
      301 taagaaagtt ttcttttctt aatttcctca ttgagagctg catagacttt gtcggcgccg
      361 gcagcaatgc cgctcgttgc catgccgaca actattttta caccgtcggg aaaaagagac
      421 tttaaacctt tctccccggc ttttttcaaa tcgtcaattg tgctgatttt tttcactttc
      481 tcacccctgt ttgctataat ttcctaatat cttgggaatt ttatcctgtg tgagatatgg
      541 atgaatttcg ccgccaatcc gcacaaccgg cgccaaagaa cagcacccga gacaccgtac
      601 ctcttccagg gaaaacatat tgtcttttgt cgtttctccg cattcgattt ccaaatcccg
      661 cttgagcttg tccattaacc tggatgcccc ctgcacgtga caggcagttc cggcacaaac
      721 ttctattaag taacggccgc ggggattcaa actaaaagaa ttataaaaag tagccacctc
      781 ataaatacgg ctggctggaa tttccatttc ttcattaacg gtggcgagcg cgtctttggg
      841 aagataatta aattgctctt gaatatcctg caaaacagca atcaaggaac tcttgtcagc
      901 tccatgtttc tttactattt cttttgtttt cgcctctatc gtagttgcgg cagtagttgt
      961 ggcttttttc atgcgtaaat cacctcagaa tttttccgga tttccccgct atatcaatcc
     1021 catgctttaa gacatttgac tttttccaaa agttttttgt attcgctatt tactctgtcc
     1081 tgaatcattt tgatttcgtg ttctcccaaa tggcggaagc gtccctgtgt tttgaaataa
     1141 tctttaatcg gctttaattt tttcggcatt tccgcggaaa tcttatactt gccgttttcc
     1201 acttcataca agggaaatat gccggtttcc acaaccaggc gtcccaaagc aatagtcaaa
     1261 tgggaaggag agcgccatcc ggtcggacaa acggagaaac aatggatgta agaagggcct
     1321 ttgacgctct tggctttttc caccttgcga ataaaatcaa gcgggtaact gggcgaggcc
     1381 gttgccacgt aaggaatgtt gtgggcaacc attatttctg gcatattctt tttccaagtc
     1441 ttttgtccgt aactgatttt acctgccggt gcagtcgttg tggaagcgcc cataggagtg
     1501 ccgctggagc gctgaattcc cgtgttcata taagcttcgt tgtcaaaaca gacaaagagc
     1561 atatcgtgtc cgcgttccat cgcccctgaa agcgcttgta gaccgatatc cattgtacct
     1621 ccgtcaccgg ccatgccgac aacttttata tagcgattgt cgatcctgcc ttttttgcgt
     1681 agcgccttga gg
//
LOCUS       DASVIM010000114         3374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_386287, whole genome shotgun sequence.
ACCESSION   DASVIM010000114 DASVIM010000000
VERSION     DASVIM010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386287"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1988)
                     /gene="msbA"
                     /locus_tag="VF305_02265"
     CDS             complement(225..1988)
                     /gene="msbA"
                     /locus_tag="VF305_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEX7415991.1"
                     /translation="MDIFKRLLKLARPYSVRFFFAMICMLIVGALTSSQAVLVKPILD
                     DIFLGKNPDALKWVPLAVIGIFFFKGVANYIQAILMSFIGLRIVTNLRNQLYEQIQKQ
                     SLSFFTQHSTGMLMSRITNDVMSIQAASSEAVTSVIKDTFSIIALVGVIFYTDWKLAI
                     IAMVVFPVTIYPISKFGKKMRKVTTSTLITMGTLSSLLQETISGTRIVKAFGMEKYEN
                     KRFATENDRLFKLNMKIVSVNAISHPLMEFLGSLGIAAVIFYGGYNVLHGNSTPGTFS
                     SFLAAVLMLYEPVKRLTNVNNTINQGIAGAERVFNIIDHVPDFEDKPDAIKLPPICRS
                     IDIENVNFSYEETPVLKNITLSIKAGEVVAFVGMSGGGKTSLVNLIPRFYDVSEGRIV
                     IDGHDIRDVSMQSLRSQIAIVTQQTILFNDSVKNNIAYGDIDRTEEDIINAAKAANAH
                     DFIMKLPEGYDANIGELGTKLSGGEKQRISIARALLKNAPILILDEATSSLDTEAEIE
                     VQDALDNLMKGRTTLVIAHRLSTIRNADRIIALVNGEIVEEGNHETLLAKKGEYFRLY
                     NLQFKDEGNNVENEQLAKDLG"
     gene            complement(1997..3196)
                     /gene="lpxB"
                     /locus_tag="VF305_02270"
     CDS             complement(1997..3196)
                     /gene="lpxB"
                     /locus_tag="VF305_02270"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX7415992.1"
                     /translation="MPTLLTMPPKEINPASAATIADKKTKTIMIVAGEASGDLHGASL
                     VREMLKTDPALNFYGIGGSKLEKMGVKLFANTSTMAVMGLTEVVSKLGSILKIMGMMK
                     KSLDEYRPDLVILIDYPDFNLRLAKAAKKKGIKVFYYISPQVWAWRKNRINQIKKFVD
                     KMAVILPFEVDTYATKGFTVNYVGHPLLDLVKPHYSKQDSRKKISIAENKTTIALLPG
                     SRLSEVTKLLPEMLRAAEILAQKIPNAQFVLPLADTLEEKNISDISKSSPVKIKVIAG
                     LTYDVISSADLAIVASGTATLETGLLGVPMIIIYKVSPFSALIGRMIINVQHIGLVNI
                     IAGKTIVPELIQSDANGQRIASEALAILLNEEKRQEIVAQLADIRAKLGEPGAARRAA
                     QLALDML"
BASE COUNT          929 a          724 c          715 g         1006 t
ORIGIN      
        1 aaccgtcctc ttgcagcatt tgcgccagca taataacgca gggagtttta cctgtgccac
       61 caacggttat attaccgacg ctaatcaccg gacaggaaag tttttcttct ggaagtatct
      121 ggtgatcgta aagccaatta cgaaaattga taatcagacg gtaaggcaaa gaaaggacat
      181 atgcaattat cttgattggg ctgtaacgac tgatacttcc gtcatcatcc caaatccttt
      241 gccaattgtt cattttccac attattccct tcatctttga attgtaaatt gtagagccgg
      301 aaatattcgc cttttttcgc cagcagtgtt tcgtggttgc cttcttcgac aatttctcca
      361 ttgactaagg ctattattct gtcagcattg cggatcgtgg aaagacgatg ggcaataacc
      421 aaagtggtgc gtcctttcat gagattatcc aacgcatcct gtacttcgat ttccgcttcc
      481 gtatccagcg atgatgtagc ctcatcaagg attaaaatgg gagcgttttt gagtagcgcc
      541 cgggcaatgg aaatcctctg tttttctcct ccggaaagtt tggtgcccaa ctcgccgata
      601 ttggcatcat agccctccgg cagtttcatg atgaagtcat gagcgttggc cgccttggcc
      661 gcattgatga tatcttcttc tgtccggtcg atatcgccat aagcaatgtt atttttcaca
      721 gaatcattaa aaagaattgt ctgctgggtg acgatggcaa tttggctgcg cagtgattgc
      781 attgaaacat cgcggatgtc atgaccgtcg atgactatac gcccttctga aacgtcgtaa
      841 aatctgggaa tcaagttgac aagcgatgtt ttcccgcccc cgctcatgcc gacaaaagca
      901 acaacctcgc cagctttaat ggaaagagtg atgtttttta gtaccggtgt ttcttcataa
      961 gaaaagttta cattttcaat atcaatgctg cggcaaatcg gcggcaactt tatcgcatcg
     1021 ggtttgtctt caaaatccgg cacgtggtca ataatgttga agacgcgctc tgcgcccgct
     1081 ataccctggt tgatggtgtt gttgacgtta gtgagtctct ttacaggctc gtagagcatt
     1141 aaaactgcag ccagaaatga ggaaaatgtg cccggagtag aattgccatg cagtacgtta
     1201 taaccaccgt aaaagattac ggcggcaata cccagcgaac ccagaaattc cattagggga
     1261 tgggaaatgg cattgaccga aactatcttc atgtttagtt taaataaacg atcgttttcc
     1321 gtagcaaatc ttttattttc atatttctcc atgccgaaag ctttgacaat gcgggtaccg
     1381 gaaatcgtct cctgtaataa agagctcagc gttcccatgg tgattagtgt ggacgtggtt
     1441 actttacgca tttttttgcc gaatttggaa ataggataaa tggtcacggg aaaaacaacc
     1501 atggcaatga tggccagttt ccaatcggta taaaaaatga cgccgaccag agcaatgatg
     1561 gagaaggtgt ctttaataac acttgttact gcttctgatg aggcggcttg aatggacata
     1621 acatcgttgg taatacgcga catgagcatg cccgttgaat gctgtgtaaa gaaagataat
     1681 gactgcttct ggatctgttc atagagttga ttacgcaaat tagtaacaat gcgtaatcca
     1741 atgaagctca ttaagatagc ttgtatgtaa ttggccactc ctttgaaaaa aaagattcca
     1801 ataacagcga gaggaaccca cttcagcgcg tcaggatttt tacccagaaa gatgtcatcc
     1861 agaataggtt tcaccagcac agcttgtgat gatgtcaggg ctcccacaat gagcatgcaa
     1921 atcatcgcaa agaaaaaacg aacactgtag ggccgggcca gttttaaaag acgtttaaat
     1981 atatccatag ttttgcttat agcatatcca atgccagttg ggcagctctt cttgcggcgc
     2041 cgggctcgcc gagcttggcg cggatgtccg ccagttgtgc gacaatctcc tgcctttttt
     2101 cttcgttcaa taaaatagcc agagcctcgg acgcgatacg ctgaccattg gcatccgact
     2161 gaatcaattc cggaacaatc gttttgccgg caataatgtt gaccagtcct atatgctgga
     2221 cattgataat cattctgccg atcagagccg aaaaaggtga aaccttgtaa atgataatca
     2281 tgggcacgcc cagcaaacca gtttccagcg tcgctgttcc cgatgcaact atggctaaat
     2341 cagcagagga aataacgtcg taggttaagc cggcaatcac tttgattttt acgggagaac
     2401 ttttgcttat atcggatata tttttttctt cgagtgtatc agccagcggc aagacaaatt
     2461 gcgcgttggg tattttttgc gccaagattt cggctgcccg cagcatctcc ggcagtaatt
     2521 tggttacttc cgataaccgg ctgcctggta aaagtgcaat tgtagttttg ttttcggcaa
     2581 tactaatttt ttttctcgaa tcctgttttg aataatgtgg cttgaccaaa tccagtaagg
     2641 gatggccgac ataatttaca gtaaaccctt ttgtcgcata ggtatcgact tcaaaaggaa
     2701 gaataacggc catcttatcc acaaatttct taatctggtt gattctgttt ttgcgccaag
     2761 cccaaacttg cgggctgata taatagaaaa cttttattcc tttcttcttc gcagctttgg
     2821 ctaaacggag attaaaatcg ggataatcaa ttaaaataac aaggtcaggt ctgtattcat
     2881 ccagagattt tttcatcatg cccattattt ttaaaatact tcccaattta gagacaacct
     2941 cggttaatcc cattactgcc atcgtggaag tattggcaaa aagttttact cccatttttt
     3001 ctaacttgct gccgccgatg ccataaaaat ttagtgccgg atcagttttt aacatttccc
     3061 ggacaaggct tgcgccgtgc agatcaccgg aagcttcacc ggcaacaatc atgatagtct
     3121 ttgttttctt gtctgctatg gttgctgctg aggctggatt tatttcttta ggaggcatag
     3181 ttaataacgt cggcaataat tttaatttct tctttataag aaattgtaat ttccaatctc
     3241 tatattactt tgaaatattg agggtataag gcttatacga actaattata acttactaat
     3301 accctgtatt ttatacagat aataattgca cagatgacta acgccaccaa agcctatttt
     3361 tttacttact atat
//
LOCUS       DASVIM010000115         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1153855, whole genome shotgun sequence.
ACCESSION   DASVIM010000115 DASVIM010000000
VERSION     DASVIM010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153855"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VF305_02275"
     CDS             <1..469
                     /locus_tag="VF305_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7415993.1"
                     /translation="AGELLLGKAFPYFFIGLTDVLIAMLLGQLLFGVVMKGNFFLMIA
                     ATSLYLAVALSLGLFISTATKSQLIANQVAILTTYLPSVLLSNFVFPIINMPKFLQLI
                     TYIVPARYYIDILGGIYLRNTGFSYLWPSMAVLMFMFTLLTSLNYLLLKKEGL"
     gene            466..1587
                     /locus_tag="VF305_02280"
     CDS             466..1587
                     /locus_tag="VF305_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7415994.1"
                     /translation="MSWLRIRELVRKEFIQLFRDKRNRITLFVAPFIQMLVFGYVVNY
                     DIRNIHVALVDYSQTYESRKLADAFEGSNIFHITHNLTDDRQMEELLLRGKVDMGIKI
                     GPDFAGIIRKGQTASIQIIADGSMSNMASMRMAYTVTVIDKFNRDTIRELYPQQMSYG
                     KIDARIRTWYNPNLDSQYFFVPGIVAFLIMLISLLLTSIAIIREKEAGTMEQLIVTPL
                     KASELIVGKTIPYIIISLAQLSAIIVVAIYWFEIPLKGSIVLLFFASCLFLLSTLGVG
                     LFISTISATQQQAMMTTFFFIMPFFMLSGFVFPIANMPTFVQWLTYLNPLRYFLVIIR
                     GIFLKGVALEVLWPQFLALAILGGLVFSGAILRFKKRLD"
     gene            1639..2631
                     /locus_tag="VF305_02285"
     CDS             1639..2631
                     /locus_tag="VF305_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415995.1"
                     /translation="MLKVIEDQLVIKKYAKRFAKKFKPLIDEEIKAKIGHQGASFRAK
                     VLWSKSLGTWKFSRTIKGTRYWNAFGVGKPQESSLLSITAEINFPWAGIDRKTGGAFA
                     EDAWGNVFVIHRGKIGGGKKGVGKSLFEHNYRGVWSFMEDGKSVTQVAIIGALNSSRF
                     ALQAAQFIKKIEKLKSTATTSTQTEINFLEITFREDLVGSAPYCIEDEIISACDRDLV
                     IGDLAALLQRRKLKIGNDTERELFVVDPSKNRISHVFEVLTDTKEKSVLAAAAKLLLQ
                     TSASAGNPLPILVLPEDKINKYEQELQRINISVIGFRWEGEKAVFPELEKIEFK"
BASE COUNT          734 a          571 c          573 g          852 t
ORIGIN      
        1 ggccggggaa ttgctgttgg gaaaagcttt cccttatttt tttatcgggc ttaccgacgt
       61 tttaattgcc atgcttttag ggcaactcct ttttggagta gtaatgaaag gcaatttttt
      121 tctgatgatt gccgctacct ccctttattt agccgtggca ctttcgttgg gtttgttcat
      181 atccaccgcg acaaaatcac agctgattgc caatcaagtg gcaattttaa ccacgtattt
      241 gccctctgtt cttctttcca attttgtttt tcctataatt aatatgccta agtttttgca
      301 gcttattacc tatatcgttc cggcaaggta ttacatcgat attttaggcg gtatctatct
      361 gcgcaataca ggattttcct atttgtggcc gagcatggca gttttgatgt ttatgttcac
      421 attgcttaca tcactgaact atttgttgtt gaaaaaggaa ggcctatgag ttggctgcgg
      481 atcagggaat tagtgcgcaa agaattcatt caactttttc gcgataagcg aaaccggata
      541 actctttttg tcgctccttt tatccaaatg ctcgtttttg gctatgtggt caattatgat
      601 attcgtaata ttcacgttgc ccttgtcgat tattcccaga cttatgaaag ccgcaaactc
      661 gctgacgctt tcgaaggcag caacattttt catattaccc ataatttaac cgatgaccgg
      721 caaatggaag aactcctgct gcggggcaaa gttgatatgg gtattaaaat cggcccggat
      781 ttcgcgggca ttatccgcaa aggccaaaca gcatcgattc agattattgc cgacggttca
      841 atgagcaata tggcttccat gcgcatggct tacaccgtca ctgtaatcga taagtttaac
      901 cgcgacacca tccgcgaatt gtacccgcag caaatgagct atggtaagat cgatgcgcgc
      961 attcgcacct ggtataatcc caatctggac agccaatact tcttcgttcc gggcattgtc
     1021 gcttttttaa tcatgctgat ttccttactt ttaacttcca tagccattat tcgggaaaag
     1081 gaagcaggta cgatggagca actaatcgta acaccgctta aagcttccga attaattgtc
     1141 ggtaagacaa ttccttacat aattatttca ctggcgcaat tatcggcgat aattgtcgtt
     1201 gccatctact ggtttgaaat accgttaaaa ggcagtattg tacttttatt ttttgcctcc
     1261 tgtttatttt tgctcagcac tctgggcgtg gggcttttca tctccactat ctccgctact
     1321 cagcagcaag cgatgatgac cactttcttt ttcatcatgc ctttttttat gctcagtgga
     1381 tttgttttcc ccattgccaa catgcccaca tttgtccagt ggctcaccta tctcaatcct
     1441 ttgcgctatt tcctggtcat tatccgtgga attttcctta aaggggttgc cctggaagtg
     1501 ctctggccgc aatttttggc gctggctatt ctgggaggac ttgttttttc cggcgcaata
     1561 ctgcgcttta aaaaacgtct ggattagtat aatattaaag tatcttaatc ttctcaaaat
     1621 tattggcggg ataattttat gcttaaagtc atagaagacc aactagtcat aaaaaaatat
     1681 gccaaacgat tcgccaaaaa attcaaacct cttattgacg aagagataaa agcaaagatc
     1741 gggcatcagg gagcgagctt tcgggcgaaa gttttatggt caaaaagtct cggcacttgg
     1801 aaattttcgc gcaccataaa aggtactagg tattggaatg cctttggtgt aggaaagccg
     1861 caggagtcaa gccttctttc cataaccgcc gaaattaatt ttccgtgggc aggaatagat
     1921 cgaaaaacag ggggagcatt tgctgaggat gcctggggaa atgtctttgt aattcatcgt
     1981 gggaaaatag gcggtgggaa gaagggtgtc ggcaaatctc tttttgagca taactaccgt
     2041 ggcgtctggt catttatgga ggatggaaaa tcagtcaccc aggtggctat aatcggcgcc
     2101 ctcaactcca gccgctttgc cttacaagcc gcccagttta taaaaaaaat cgaaaaacta
     2161 aaatcaactg ctacaacatc aacgcaaact gaaatcaatt ttttggaaat aacttttcgg
     2221 gaagatttag tgggaagcgc gccttattgt attgaggatg aaattatttc cgcgtgtgac
     2281 cgtgatttag tcatcggcga cttggccgct ttacttcaac gccgtaaatt aaaaataggc
     2341 aacgacactg agcgtgaact ttttgtggtc gatccttcta aaaacagaat ttctcacgtt
     2401 tttgaagttt taaccgatac aaaagaaaaa agcgtattgg ctgcagcggc caaattgctt
     2461 ttgcagactt ccgcctctgc aggaaatcca cttcctattt tggttttacc ggaagacaaa
     2521 attaataaat atgaacaaga actacagagg ataaatattt cggttattgg ttttcgttgg
     2581 gagggagaaa aagccgtttt cccggaatta gaaaaaattg agttcaagta atttgaaaaa
     2641 gtatttacta tctgctgccg gcttcaagat tatccatgat ttgccgcata tatttttcta
     2701 taatgacaaa ctgattgtcc tgcgccagca
//
LOCUS       DASVIM010000116         5744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993 k141_44556,
            whole genome shotgun sequence.
ACCESSION   DASVIM010000116 DASVIM010000000
VERSION     DASVIM010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44556"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..459
                     /locus_tag="VF305_02290"
     CDS             256..459
                     /locus_tag="VF305_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7415996.1"
                     /translation="MKKVFAIIISVLFVASFAVVGCKKEEPKPAEAPKVEAPAAQPAP
                     GAPGAPGAPGAPGAPGAPAPAQK"
     gene            809..1192
                     /locus_tag="VF305_02295"
     CDS             809..1192
                     /locus_tag="VF305_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX7415997.1"
                     /translation="MSERNGGLLKGLIIGGLIGAVLGVLYAPKSGKEMREELAGKTDD
                     LLSKAKEEYEKALEKSRIVSETAVKRLEELGSSAKEKVEEAESKVNKFAYQGVKAVPG
                     NKNKLKKAFDVGVATYRKEKNKKLI"
     gene            1210..1599
                     /locus_tag="VF305_02300"
     CDS             1210..1599
                     /locus_tag="VF305_02300"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEX7415998.1"
                     /translation="MYLEICLIILGIAFVLLVIFYIPILMQIWLTSKNITVTLETLNQ
                     RLPTILKNLEEITTNINSSTAAVNREIQNVSNTVDRFHLVVDNIVDDIKNIAPMAGKF
                     RFFQMIKNTIAIAKGVRVFMDVFLHKK"
     gene            1622..2089
                     /locus_tag="VF305_02305"
     CDS             1622..2089
                     /locus_tag="VF305_02305"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HEX7415999.1"
                     /translation="MAMVTFSSKWGKVKVQKKPVLHAAAAEKSVEESVREMKKAQKPA
                     AGEDYREKSLAIHGLVCALCGREFDESKRQLLTVHHKDGNHHNNPADGSNWENLCVYC
                     HEDAHSRGVLAEYLGNNAHGREVSLVYEDSPAGGSSGLLAEKLKKALQKSKSQ"
     gene            complement(2095..2292)
                     /locus_tag="VF305_02310"
     CDS             complement(2095..2292)
                     /locus_tag="VF305_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HEX7416000.1"
                     /translation="MRESYKDFDATELFCPQCRRAVPVRKRLLLILPEGEKYDYNCVY
                     CGTSVGDKLIKEKGNLSVIVR"
     gene            complement(2295..4364)
                     /locus_tag="VF305_02315"
     CDS             complement(2295..4364)
                     /locus_tag="VF305_02315"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX7416001.1"
                     /translation="MPAKKVSNFFNLYNHGFIRAAVCIPELKVADTFFNTQKTIELAR
                     QAVSQKAILAIFPELGLSAYSNEDLFHQDALLQSVREALIKIKMASEKINLIMAVGAP
                     LQVDCRLYNCAVIFYRGKILGVAVKSYLPNYREFYEARQFTPAAAALSSKIDLAGQKD
                     IPFGADIIFKVANINNFNFFLEICEDVWVPIPPSSFAAMAGATVIGDLSASNITIGKS
                     EYRHQLAANQSARLVAAYLYAAAGTGESTTDLAWDGHAMIYENGNLLAESTRFSQQAQ
                     IIYSDIDLDRLAQDRMRLTSFGQNATTHIEKIAAFRRVEFDVDLFSGRLILNREYARF
                     PYIPADPSKRYQRCYEAYNIQVQGLAKRLKASGIVNLVIGVSGGLDSTHALIVAAKTM
                     DLLGLSRSNVKAYTMPGFATTTKTYNNALKLMKALGVEANEIDIKPSCLQMLKDIKHP
                     FAQGKKVFDITFENVQAGQRTAHLFRLANAKNALVVGTGDLSELALGWSTYGIGDHMS
                     HYNVNASVPKTLIQHLIRWVAQTNQFSPEASKTLIDVLETEISPELVPGKIGNQPGQR
                     TEDVIGPYELQDFNNFYITRFGYLPTKVAFLAYRAWRDKTKGLWPDIPLKKRNAYTLK
                     EIKKWLYVYLYRFFKTSQYKRSCVPNGPKVGSGGSLSPRSDYRAPSDSEAEVWLTNWE
                     KIPDTED"
     gene            complement(4369..5136)
                     /gene="folE2"
                     /locus_tag="VF305_02320"
     CDS             complement(4369..5136)
                     /gene="folE2"
                     /locus_tag="VF305_02320"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEX7416002.1"
                     /translation="MIDIQNQKDFRNIDIKKVGVKNIKYPIIVLDRARGTQPVNATIN
                     MYVNLPRHFKGTHMSRFVEVLNEFKGQISIKTFQTILEKTRQKLHAHSAHMEIEFPYF
                     LEKTAPVSKAKSLMEYRCLFSGENSADQTDFLVEIVVPVTTVCPCSREISKFGAHNQR
                     SIVTTKVRFKKFFWIEDIIKIVEKSASGEVYSLLKRVDEKYVTEKAYENPKFVEDVVR
                     SIAVKLDKDDNFIWYCIEAENFESIHNHSAYACVEKG"
     gene            complement(5303..5680)
                     /gene="queD"
                     /locus_tag="VF305_02325"
     CDS             complement(5303..5680)
                     /gene="queD"
                     /locus_tag="VF305_02325"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEX7416003.1"
                     /translation="MYEVTIIKSFSAAHIHAEIGGKCEELHGHNFKVEVTVAAQELNP
                     AGLLIDFRVVKKWLNEILEDMDHKHLNSQPSFAGINPSAENIAKYISGKIQLKAKPAQ
                     VHVVRVKVWESENAAVTYIPEEG"
BASE COUNT         1630 a         1125 c         1298 g         1691 t
ORIGIN      
        1 gtttttacag agtattgcct gggaattctt tatgagcgga aagctgtatc ttctctttag
       61 aataatttaa ttaaagtatt aatattggat gggaattctc ttgctataac actaatgatt
      121 aaaaagaaat ggtgcaattt atacgtattg taaatttgtc cttgacaagg caaataaaaa
      181 gcgtattctt tcacattaaa attggggtat gaaaacccaa ggataagaaa ttaactgaaa
      241 ggaggacaga cgttaatgaa gaaggtattt gctattatta tttcggtgct tttcgttgct
      301 tcttttgcag tagtaggctg caaaaaagaa gagccgaaac ccgcagaggc tcccaaagtt
      361 gaagcccccg ctgctcagcc ggcacctggc gcacccggag cacctggcgc acccggagca
      421 cctggagcac ctggcgcacc ggcacctgca cagaaataat tatttctaat tcataagcag
      481 gacaagttaa ccggatattc gttgttttaa tgaatgtccg gtttttttgt gccaagtatg
      541 gtttaatctt gaaaaatgtg ttagattata gtcaataaaa ttggattgtc tttggtggtt
      601 atatcattaa acttctgtgt ataattagaa atctctggct cggttctttc tcgtcgtggt
      661 ggtaagctat tgactgtgga agtgctattc gaaggagtgt aatgaaaaaa tctttgctta
      721 tgcgggaagt attgccctcc ggatgggaag ttccctgcat gtggatgtaa aatgttaaga
      781 gtctttagaa taatttcggg ggtgttttat gtcggaaaga aatggcggtt tattaaaggg
      841 tctaattatt gggggattga tcggcgcagt tttgggagtt ttatatgcac cgaaaagtgg
      901 taaggaaatg cgagaagagc ttgctggcaa gacagatgat cttctgagca aggccaagga
      961 agaatatgaa aaagccttag aaaaaagcag aatagtatcc gaaacggcag taaaaaggct
     1021 ggaagaattg ggatcatcag caaaagaaaa agttgaagaa gcggaaagta aagtgaataa
     1081 atttgcttat caaggtgtca aagcagttcc aggcaacaaa aacaaattga aaaaggcatt
     1141 tgatgttgga gtagcaactt accgaaaaga aaagaataag aaacttattt aattattgag
     1201 gataaatcca tgtatctgga aatttgtttg ataattttgg gcattgcctt tgttctattg
     1261 gtaatttttt acattcccat tcttatgcaa atatggctca cctccaagaa tattacagta
     1321 actctggaaa ctttaaatca aaggttacct acaatattaa aaaatctgga agaaattacc
     1381 acgaacatta atagttccac tgctgctgta aatcgggaaa ttcaaaatgt ttccaatacg
     1441 gtagacaggt tccatttagt tgtagataat attgtcgatg atatcaaaaa catcgctccg
     1501 atggccggga agtttcgttt ttttcaaatg attaaaaata ctattgcgat tgcaaaagga
     1561 gtgcgcgttt ttatggatgt atttttgcat aagaaataag gatgttccgg gaaggattgt
     1621 aatggcaatg gtaacatttt ccagcaaatg gggaaaggtt aaggttcaga aaaagccggt
     1681 tttacatgct gcagcagcgg aaaaatcagt tgaagaatca gtccgggaaa tgaaaaaggc
     1741 gcaaaaacct gcagctggtg aggactatcg agaaaaatcg ttggcgattc atggtcttgt
     1801 ttgcgccctt tgcggccgtg aatttgatga atccaaaaga cagcttctga ccgtacacca
     1861 caaagacggc aatcaccaca ataatcctgc cgatggcagc aactgggaaa atctttgcgt
     1921 ttattgtcat gaagatgccc atagccgggg tgtattggcg gaatacctgg gtaataatgc
     1981 tcatggccgg gaagtcagct tggtatatga agattcgccg gctggcggca gttccggatt
     2041 actggccgag aagttaaaaa aagccctgca aaaaagtaaa tctcaataaa gtgctcaacg
     2101 tacaattacg cttaaatttc ccttttcttt gatcagtttg tcaccgacgg aagttccgca
     2161 ataaacacaa ttataatcgt atttctctcc ttccggcagg attaacaaca gtctttttct
     2221 tacaggaact gcccgccggc attgagggca aaataattct gtagcatcaa agtctttata
     2281 agattctctc atctttaatc ttcagtatct ggaatttttt cccagtttgt tagccatact
     2341 tcggcttcac tgtcactcgg agcgcgataa tcgctgcgcg gcgaaagcga cccgccggaa
     2401 cctacttttg gcccattggg aacgcaggag cgtttgtact gagaagtttt aaaaaatcta
     2461 tacaaataaa cgtagagcca ttttttaatc tcttttaagg tataagcgtt tctcttcttt
     2521 agggggatgt ccggccaaag tccttttgtt ttatccctcc aggcgcggta tgctagaaac
     2581 gctacttttg tgggtaaata gccaaagcgg gttatgtaaa aattattaaa atcctgcagc
     2641 tcatagggac cgataacatc ttcagttctc tggccgggct ggttgccaat ttttccaggt
     2701 acaagttcgg gcgaaatttc cgtctccagg acatcaataa gagttttgga tgcttccggc
     2761 gagaattgat ttgtttgcgc aacccagcga atcaaatgtt ggataagtgt tttcgggacg
     2821 ctggcattga cattataatg cgacatatga tcgcctatgc cgtatgtact ccagcctaag
     2881 gccagctcac ttaaatctcc cgtacccaca accagcgcgt ttttcgcgtt agccagacga
     2941 aagaggtggg cggtgcgctg accggcttga acattttcga aggttatgtc gaaaactttt
     3001 ttgccctgcg caaagggatg tttaatgtct ttgagcattt gcaagcaaga gggcttgata
     3061 tctatttcat tggcctctac gcctaaggct ttcattaatt ttagagcatt gttataggtt
     3121 tttgttgtag tggcaaagcc cggcatagtg tatgccttaa cattcgagcg ggataaacct
     3181 aaaaggtcca ttgtcttggc ggcgacaatt agagcatggg tagaatcaag gccgccggaa
     3241 acgccgataa ctaaattgac aatacccgaa gcttttaagc gtttcgccaa accctgaact
     3301 tgaatattat aagcttcgta acagcgctgg tagcgcttcg atggatcagc gggaatatac
     3361 gggaaacgag cgtattcgcg gtttaaaata agacggcctg aaaagagatc aacatcaaat
     3421 tcgaccctgc ggaacgcagc aatcttttct atgtgggttg ttgcattctg gccaaatgaa
     3481 gttaagcgca tccgatcttg cgccaaacgg tctaaatcga tatcactata aattatttgg
     3541 gcttgttgtg aaaagcgggt agattctgca agcagattgc cattttcata aatcatggcg
     3601 tggccatccc aggccaggtc ggtagttgat tcaccggtgc cagcggcggc gtaaagatat
     3661 gcggcaacta agcgcgccga ttgattggcg gccagttggt gacgatattc ggatttcccg
     3721 atggtgatgt tggaagcaga cagatcgccg ataacagtgg ctccggccat agcggcaaaa
     3781 ctcgatggtg ggataggaac ccatacatct tcgcaaattt cgaggaaaaa attaaaattg
     3841 ttaatattgg caactttaaa aataatatca gcgccaaaag gtatgtcttt ttgtccggct
     3901 aaatctattt tggaggaaag cgcagcagcg gcaggcgtaa attgtcttgc ctcgtaaaat
     3961 tcgcgataat tgggcagata tgattttaca gcaacaccca aaattttacc gcgataaaaa
     4021 ataacagcac aattatatag ccggcaatct acctgcaggg gcgcgcctac agccataatc
     4081 aagtttatct tttcggaagc cattttaatt tttatcaagg cttcccgaac actttgtagc
     4141 agtgcatcct gatgaaatag atcttcatta gaataagcag ataaacccaa ttccgggaaa
     4201 atagctaaga ttgccttttg ggaaacggct tgcctggcta gttcaatagt tttctgagta
     4261 ttaaaaaaag tgtcggcaac cttgagctcg ggaatgcaaa cagcggcgcg gataaaacca
     4321 tggttatata aattaaaaaa attactcact ttttttgctg gcatgagttt atcctttctc
     4381 tacgcaggcg taagcgctgt ggttatgaat gctttcaaag ttttccgctt caatgcaata
     4441 ccagatgaaa ttatcgtctt tgtctagttt tacggcaata ctgcggacga cgtcttcgac
     4501 aaatttaggg ttttcgtaag ccttttccgt gacgtatttt tcatcaacac gctttaaaag
     4561 agagtatacc tcaccgctgg ccgatttttc cactattttt ataatatctt cgatccaaaa
     4621 aaatttttta aagcgcactt ttgtcgtaac aatactgcgc tggttatgag cgccgaattt
     4681 gctgatttcg cgagaacagg ggcagacggt cgtaacggga accacaattt caaccagaaa
     4741 atcggtttgg tcagcgctat tttctccaga gaaaagacac cggtattcca tgaggctttt
     4801 ggccttggaa acaggcgctg ttttttccag aaagtagggg aattcaattt ccatgtgggc
     4861 ggaatgggca tgcagctttt gcctcgtctt ttctaaaatt gtttgaaagg ttttaatgct
     4921 gatttggcct ttgaattcat tgagcacttc cacaaagcgg ctcatgtgtg tgcccttaaa
     4981 gtggcgcggc aggttgacgt acatatttat ggtggcattg accggctggg tgccgcgggc
     5041 acgatcaagg acaataattg gatatttaat attctttaca ccgacttttt taatatcgat
     5101 attgcggaaa tctttttgat tttgaatgtc aatcatttaa tttaactttt tttaggtatt
     5161 tagccccggc attcgagact gtgtcgtaat cctcatttgt cattgcgcgt ccgccgtggt
     5221 ggattgcttc gttcgctttg ctccctcgca aagacattgc gacacagtct cgttggccgg
     5281 gctacgcttc cgtcttgaac ctttagcctt cctccggtat gtacgttact gctgcgtttt
     5341 ccgattccca aactttaacc cgaacgacat ggacctgtgc cggtttagct tttagttgta
     5401 ttttcccgct aatatattta gctatatttt ccgctgatgg gtttatgccg gcaaatgaag
     5461 gttggctatt gagatgtttg tggtccatat cttccaggat ttcattgagc catttcttca
     5521 cgacgcggaa atcaattaac aaaccggccg ggtttaactc ctgcgccgcc acagttacct
     5581 ctactttaaa attatggccg tgtaattctt cgcatttacc gccaatttcc gcatggatat
     5641 gcgcagcgga aaatgatttt attattgtca cctcatacat cttcaactct ccccaatcgt
     5701 caatatgtga ttactaccat attttactgt gcgaggaaat aatt
//
LOCUS       DASVIM010000117         2866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_472144, whole genome shotgun sequence.
ACCESSION   DASVIM010000117 DASVIM010000000
VERSION     DASVIM010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472144"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /gene="lepB"
                     /locus_tag="VF305_02330"
     CDS             complement(<1..501)
                     /gene="lepB"
                     /locus_tag="VF305_02330"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7416004.1"
                     /translation="MAEDNKSKTKEKTKSKVQEYIEAIIIAILIALVIRTFVIQAFKI
                     PSRSMVPTLLVGDQILVNKFIYGVKIPYLRKTIIPITAPQRGDIVVFIYPNDRSKDFI
                     KRVIGVAGDKIEIKNKKIFINGKEYSDSYGVYSDNLIYPAAIQPRDNFGPVIVPQGSL
                     FVMGDNR"
     gene            complement(516..1028)
                     /locus_tag="VF305_02335"
     CDS             complement(516..1028)
                     /locus_tag="VF305_02335"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEX7416005.1"
                     /translation="MSAFTAAGLTIFILMLFAGIYLILFDLPGTVVIFFAVLLYALFT
                     GFNQVSGKIILFLLFISIIAETIDFFLVMGGALQPVASKKSFWTAALGAILGAFLLTP
                     FFWGPGTWGGFFLGGLAGILTIEFIRQFKLKASYRASNRAIFAMVGRKIAKGFIALFM
                     IALSLSNIYS"
     gene            complement(1030..1938)
                     /gene="secF"
                     /locus_tag="VF305_02340"
     CDS             complement(1030..1938)
                     /gene="secF"
                     /locus_tag="VF305_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX7416006.1"
                     /translation="MELIKPGIHLDFVGKMKYAIIFSAILTIISIASVIIHGGLNLGI
                     DFAGGSIIQVKFQKDMTADKIREALKSTRLENSIIQQFGPNEFLIRTAESFHDQILLE
                     ENVNKPLTAAFNKDYEIRRVEVVGSKVGMDFQRKAIFAVILSWLAMLIYIAWRFEFRY
                     GLGGIIALVHDVTIIIGTISILKMEFTISILAAIFFIIGYSINDTIVVLDRIRENVKL
                     NPRQKLAETINMSINQTLSRTILTSFTVFLTVTALYLFGGPVLHDLAFSLLVGVVFGT
                     YSSVFISCPIVLLFENMQLSKIKRKK"
     gene            complement(1969..>2866)
                     /gene="secD"
                     /locus_tag="VF305_02345"
     CDS             complement(1969..>2866)
                     /gene="secD"
                     /locus_tag="VF305_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX7416007.1"
                     /translation="RADRSTGQKGSVPYLLKNKALLTGASLETAKVQISDRFGEPNVS
                     IKFNSQGAIDFDRITGENVRKHLAIVLDGVVHSAPVIQERISGGQAQITGNFTMDEAR
                     DLAIVLRAGALPAPVNILEERTVGPSLGSDSIRQGIMATLIGSLLVILFMIIYYRLSG
                     TVADLALIINIVLVLGVLAAFKATLTLPGIAGLLLTVGMAVDANVLIFERIREELRTG
                     KTIRLSLDTGYKRAFITIIDTHVTGIVSAIFLIIFGTGPIKGFAVTLIIGLLASLFTA
                     VFVTRVIFDYFTWNFNVKKLSI"
BASE COUNT          902 a          602 c          585 g          777 t
ORIGIN      
        1 gcgattatca cccataacaa aaagcgaacc ctgcggaaca atcaccggac caaaattatc
       61 gcgtggctga attgcagccg gataaattaa attgtcgcta taaacgccat aagaatcaga
      121 atactcttta ccgttgataa atatcttttt gttcttaatt tcgattttat ccccggccac
      181 accgataacg cgtttgataa aatccttgga acgatcattg ggatagataa atactacaat
      241 gtcacctctt tgaggggcgg taattgggat tattgttttt cgtaaataag gtatctttac
      301 accataaata aatttattga ctagaatttg atcgccgaca agcaatgtcg gcaccatgga
      361 acgcgaaggt attttgaacg cttggatgac aaatgtccgg ataacaagag cgatcaaaat
      421 tgcaataata attgcttcaa tatattcttg aacttttgat tttgtttttt cttttgtttt
      481 tgatttatta tcttcagcca tattaactat aacctctaag agtaaatatt ggatagcgaa
      541 agggcgatca taaaaagggc aatgaatcct ttagctatct ttcttccgac catagcaaaa
      601 atagcgcgat tagatgctcg ataagaggct ttcaatttaa attgccggat aaattctatt
      661 gtcaatattc cggcaagacc tcctaaaaaa aatcctcccc aggtgcctgg tccccaaaaa
      721 aacggtgtaa gcaaaaatgc tcccaaaata gctcctaatg ccgcggtcca aaaagatttt
      781 tttgaagcaa ccggttggag tgctccaccc ataaccaaaa agaaatcgat tgtttcggca
      841 attatagaaa taaacagcag aaacaaaatt atttttccgc tgacttgatt gaagccggta
      901 aaaagagcat aaagcaaaac agcaaaaaaa atgacgacgg ttccaggcaa gtcaaataat
      961 attaaataaa tgcctgcgaa caacatcaaa ataaaaatag tcaatcccgc tgcggtgaag
     1021 gctgacaatt tatttcttcc tctttatctt ggaaagttgc atgttttcaa aaagcagtac
     1081 aatcggacag gaaatgaata cggaagaata cgtgccaaac acgacgccga ccagcagcga
     1141 aaaagccaaa tcgtggagta ccggtccacc gaacaaatac agcgcggtca cagttaaaaa
     1201 cactgtaaac gaagttaata tggttctgct taatgtttga ttaatactca tgttgatagt
     1261 ctcggccagt ttttgccgcg gattcaactt tacattttcc cttattctat cgagcacaac
     1321 gatcgtatca ttgatagaat aaccaatgat aaagaatatt gccgcgagaa tggaaatggt
     1381 aaattccatc tttagtatgg aaattgtgcc gatgataata gtgacatcat gaacaagagc
     1441 aattatacca cccagtccat agcggaattc aaagcgccag gctatataaa tgagcatggc
     1501 cagccaggaa agaatgacgg caaaaatggc ttttctctgg aaatccatac ctacttttga
     1561 accgacgact tccacgcgtc gaatttcgta atctttattg aaagccgcgg tcaatggttt
     1621 attcacgttt tcttctaaaa gtatttgatc atggaaagac tccgccgtgc gaatgagaaa
     1681 ctcgttgggg ccgaactgct ggatgatgct gttttccaga cgggttgatt taagagcttc
     1741 cctgatcttg tcggcagtca tatccttctg aaattttact tgaattatag aaccaccagc
     1801 gaaatcaatt ccaaggttta aaccgccatg gatgataaca gatgcaatac tgatgattgt
     1861 caggatagcc gagaaaatta ttgcatattt cattttcccg acgaaatcta agtgaattcc
     1921 tggtttgatt aactccatac tatatctcct gcctgctaat gtatttgttt aaatactcaa
     1981 tttcttgacg ttaaagttcc aggtaaaata atcaaaaatt accctcgtga cgaaaacagc
     2041 ggtgaagagg ctggccagaa gaccgatgat cagcgttacc gcaaatcctt taatcggacc
     2101 ggtgccaaag ataatcagga aaattgctga aacaatgcca gttacatgcg tatcgataat
     2161 ggtaataaaa gctcttttat aaccggtatc caatgagagt cgaatcgttt ttcctgtccg
     2221 cagttcttct ctgattcgtt caaaaatgag cacgttggca tcaaccgcca taccgacagt
     2281 gagcagcaga cctgcaattc caggcaacgt cagcgtggct ttgaaggccg ccagtacgcc
     2341 caaaacaaga acgatgttga taatgagggc caaatccgcg acagttcccg aaagacggta
     2401 atagatgatc atgaataaaa taaccaatag tgagccgatg agtgtggcca taataccttg
     2461 cctaatcgag tcgctgccga gtgatggacc aaccgttctt tcttccagaa tattgaccgg
     2521 tgcgggtagt gcaccggcgc gtaaaacgat cgctaagtcg cgcgcttcat ccatggtgaa
     2581 gttaccggta atttgcgcct gcccgccgga tattctttcc tggatgacgg gtgcggaatg
     2641 gactacgccg tccaagacaa tagctaagtg tttgcgaaca ttttctccgg taatccgatc
     2701 gaagtcgata gcaccttggg aattaaattt tatagacaca tttggttcgc cgaaacggtc
     2761 ggaaatttgc actttggcag tttccaaaga ggcaccggtt agaagcgcct tgtttttcaa
     2821 caaatatggt acggaacctt tctgtccggt tgatctgtcg gcacgc
//
LOCUS       DASVIM010000118         4881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_642987, whole genome shotgun sequence.
ACCESSION   DASVIM010000118 DASVIM010000000
VERSION     DASVIM010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642987"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /gene="argB"
                     /locus_tag="VF305_02350"
     CDS             complement(<1..442)
                     /gene="argB"
                     /locus_tag="VF305_02350"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX7416008.1"
                     /translation="MEHILSSMERADILLEALPYIKRFYNKTIVIKYGGHAMVDNELK
                     DKFAQDIVMMKYIGINPVVVHGGGPQIGSFLKKLGKESKFVQGMRVTDEETMDIVEMV
                     LVGKINKEIVGLINSHGGNAVGLSGKDGNLISAEKYLLSEDKAKH"
     gene            complement(455..1849)
                     /gene="hslU"
                     /locus_tag="VF305_02355"
     CDS             complement(455..1849)
                     /gene="hslU"
                     /locus_tag="VF305_02355"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX7416009.1"
                     /translation="MSNNGLKPREIVERLDRHIIGQADAKRAVAIALRNRWRRQNVSK
                     ELADEISPKNIIMIGPTGVGKTEIARRLAKLDNSPFLKVEASKFTEVGYVGRDVESMI
                     RDLVELSVNMIKSEEQENVRTKAAEIAEEKLLDLLLPPRPAEKMVSDMLANPNDSPEN
                     AQITEEQTQRFDETREKLRKMLKSNKLDNRIVDLEVTEARSGPMIEIFSAAGMDDMGL
                     NIRDMLGNVFPQKKKRKTVKVPEALEILAEEEAQKLIDMDKVTKTAMESVEQSGIIFL
                     DEIDKIVGSDTQHGPDVSREGVQRDLLPIVEGSNVNTRYGMVKTDHILFIAAGAFTAS
                     KPSDLIPELQGRFPIRVELDSLGKEDFIRILTEPNNALVKQYTEMMATEGLKLSFTEE
                     AIASIAEVATLVNERTENIGARRLYTIMEKLLDDISFEAPDMQKKEIIIDAKNVEEKL
                     DDIVEDEDLSRYIL"
     gene            complement(1853..2437)
                     /gene="hslV"
                     /locus_tag="VF305_02360"
     CDS             complement(1853..2437)
                     /gene="hslV"
                     /locus_tag="VF305_02360"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX7416010.1"
                     /translation="MHIKGTTILAVKHNGKISVAGDGQVTLDATILKHGARKVRRLYN
                     NEVIVGFAGATADAFTLFDRFDQKLEQYNGNLLRAAVELTKDWRTDRVLRHLEALMIA
                     VSKDHSLIISGNGDVIESDDDVMAIGSGGPYAQAAARALVRHSNLPSTEIAKEAMRIA
                     AEICIYTNDHITLEEIDLSAETEERKNSKDKGQK"
     gene            complement(2489..3406)
                     /gene="xerC"
                     /locus_tag="VF305_02365"
     CDS             complement(2489..3406)
                     /gene="xerC"
                     /locus_tag="VF305_02365"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX7416011.1"
                     /translation="MENLIQDFQVYLEVERNVSVHTRKAYIADIQEFARFLHSNSFIK
                     KTDEIIKMELETIRAYLGYLYRKKVKKVTVNRKVSSLRAFYKYALRTGKIKNNPAEMI
                     QTSKAEKYMPTFLSVDETFQLLDAPGGKSSLDLRSRAMLELFYSSGLRLSELAGLDVI
                     DIDFNQELVKVRGKGKKERIVPVGRRALQAIKEYLEKTGELRRNMDVNIFKKPLFLNA
                     QGKRITTRSIARIVNEAASKSGIGRKISPHALRHSFATHLLNAGADLRSIQELLGHES
                     LSTTQKYTAVNINRMMEIYDKAHPRAKKT"
     gene            complement(3412..3912)
                     /locus_tag="VF305_02370"
     CDS             complement(3412..3912)
                     /locus_tag="VF305_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX7416012.1"
                     /translation="MLIEMKVSGLTIDPITNTPIVILKDLQESKAIPIWIGLFEASAI
                     ATELEKVVFSRPMTHDLFNECLKTLDVTVNKIVLVDIRNNTFFANIYLSKEGQNFTID
                     ARPSDAIALALRAHAPIFVDETVIEKSRSVDFGIKISDLDKLKEDKIKEFLENLSAED
                     FGKYKM"
     gene            complement(3924..>4881)
                     /gene="miaB"
                     /locus_tag="VF305_02375"
     CDS             complement(3924..>4881)
                     /gene="miaB"
                     /locus_tag="VF305_02375"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX7416013.1"
                     /translation="KITQIGFQKSLNSIGIFAPPEKGAVSVFVTIMQGCNNFCAYCVV
                     PYLRGPEISRSPKDIIEEIKKLADCGIKEVTLLGQNVNSYGKMLANGLNFTALIKKIG
                     KISGIERIRFTTSHPRDLSEELINCFAEEEKLCDHIHLPVQSGSNRVLALMNRGYTVE
                     EYAEKVDHLRKVCPQISITSDIIVGFPGEGKNDCQETIDMMGKIRFDSVFSFKYSERK
                     GTTAQKLEGKIPEDEKLIRLQQLQELQDQHTLEKNTALEGSRQELLVEGKSKNSENDL
                     MGRTRSWKIVNFKGDPGLTGKLVDVKIFKAYLHSLRGKIIDN"
BASE COUNT         1253 a         1174 c          826 g         1628 t
ORIGIN      
        1 tatgtttcgc tttatcctca ctcaaaagat atttttctgc agaaatcaaa ttaccgtcct
       61 taccgctgag acccacagca ttaccgccat gactgttaat caagccgact atctccttat
      121 tgatctttcc aaccaaaacc atttccacga tatccatggt ttcttcatca gtaacccgca
      181 taccctgaac aaattttgat tctttaccga gcttttttaa aaagcttccg atttgcgggc
      241 cgccaccatg aacgactaca ggattaatgc cgatatattt catcatgacg atgtcctggg
      301 caaatttatc ctttaactcg ttatcaacca tggcatggcc gccatattta atgacaattg
      361 ttttattata aaatcgcttg atatagggca gagcctccaa aagaatgtcc gccctttcca
      421 ttgaacttaa aatatgttcc attgtaaact ccgtttatag aatatacctg cttaaatcct
      481 catcttccac tatgtcatct agcttttctt ccacattctt ggcatcaatt attatttctt
      541 ttttttgcat atcgggcgct tcgaaagaaa tatcatccaa taatttttcc attatcgtat
      601 agagtcttcg agcgccgata ttttctgttc tttcgttaac caatgttgcc acttccgcaa
      661 tagaagctat ggcttcctca gtaaatgata attttagtcc ttctgttgcc atcatttccg
      721 tatattgctt aaccaaagca ttattgggtt cggtgagtat tcggataaag tcttctttgc
      781 ccagcgaatc aagctccacg cgaatcggga aacgtccctg caattccgga attagatcgg
      841 aaggtttgga ggcggtaaac gctccggccg ctataaaaag aatgtgatca gttttgacca
      901 ttccataacg ggtattgacg ttggaacctt ccacgatggg gagcaaatct ctttgcacgc
      961 cctcgcgcga cacgtccggt ccatgctgtg tatcactgcc cacaatctta tcaatttcgt
     1021 ctaaaaatat tatccccgat tgctcgacac tttccatggc agttttagtc accttatcca
     1081 tatcgatcaa tttttgcgcc tcttcttccg ccagtatttc caaagcttcc gggactttta
     1141 cggttttcct tttctttttt tgtgggaata catttcctaa catatcccta atattaagtc
     1201 ccatgtcatc cattcctgcg gcggaaaata tttcgatcat aggaccactt ctggcttcgg
     1261 taacttccag atctactatc ctattatcta acttattact tttaagcatt ttccttaatt
     1321 tttcccgcgt ctcatcgaac ctttgcgtct gctcttcggt aatttgagca ttttcaggcg
     1381 agtcattggg attagccagc atatcgctca ccattttttc cgccggcctt ggaggaagca
     1441 agagatcgag taacttttcc tccgcaattt ctgcggcctt agtgcgaaca ttctcctgct
     1501 cttccgattt gatcatgttt acggatagtt caaccaaatc acggatcatt gattcgacgt
     1561 ctcgccctac atagccaact tcggtaaatt tggacgcctc cacttttaaa aaaggtgaat
     1621 tatccaattt ggccaacctg cgggcaattt ctgttttccc cacgccggta ggcccgatca
     1681 tgataatatt ttttggtgaa atttcatcag ccaattcttt cgacacgttt tgcctgcgcc
     1741 atctgttgcg tagtgcaatg gccacagccc gtttggcatc agcctgaccg ataatatgcc
     1801 gatcaagtct ttccactatt tcccgaggtt ttaacccatt attggacata ctttattttt
     1861 gccccttatc tttactattt tttctttctt ctgtttcagc gcttaaatct atctcttcca
     1921 gtgttatatg atcattagta taaatacaaa tttccgccgc tattctcatt gcttcttttg
     1981 ctatttctgt cgaaggcaaa ttcgaatggc gcactagtgc gcgcgccgcc gcttgagcat
     2041 aaggtccgcc ggagccgatg gccataacgt cgtcatcaga ctcaatcaca tctccatttc
     2101 cggaaatgat gagcgagtga tccttgctta cggcaatcat caacgcctct aaatgtctta
     2161 aaacacggtc tgtgcgccaa tctttcgtca actccactgc cgctctcaac aaattgccgt
     2221 tgtattgctc caacttttgg tcaaacctat caaataaagt aaacgcgtct gccgttgctc
     2281 cggcaaaacc tacgatgact tcgttattgt aaaggcggcg aactttgcgg gcaccatgtt
     2341 tcaagatagt cgcatccaac gtaacctggc catcgccggc tacggatatt ttgccattat
     2401 gcttgaccgc caatattgtg gttcccttaa tatgcatttt cctctccctt aaaaaaacca
     2461 ggcattttaa cctatcactg tcttcaaatt atgttttctt tgctcgcgga tgcgctttat
     2521 cgtaaatttc catcatgcgg ttaatattta ccgccgtata tttttgagtt gtagaaaggc
     2581 tttcatgccc tagcaattct tgaatagaac gtaaatccgc gccggcgttt aagagatgcg
     2641 tggcaaaact gtgtcttaag gcatgcggac tgatctttct gccaatgccg cttttactgg
     2701 ctgcctcatt cactatacgg gcaatacttc ttgttgttat tcttttgcct tgagcattta
     2761 agaacaacgg ttttttaaaa atattcacat ccatatttct tcttagctct ccggtttttt
     2821 ccaaatattc tttaatcgct tgcaaagccc ttctgccaac ggggactatt ctttcttttt
     2881 taccttttcc ccgcacttta accaactctt gattaaaatc aatgtctatt acatccagtc
     2941 cggccagttc acttaagcgc aatcccgaag aataaaacaa ttccagcatc gcccggctac
     3001 gtaaatctaa agacgatttt ccccctgggg catcaagaag ttgaaatgtc tcatcaaccg
     3061 acaaaaaagt gggcatatat ttctctgctt tcgatgtttg gatcatttcc gctggattat
     3121 ttttaatttt accagtacgt aatgcatatt tataaaaagc tctcaacgac gatactttac
     3181 ggttaaccgt aacttttttt acctttttgc ggtataggta gcccaaatat gcccgaatag
     3241 tttccagctc cattttaatt atttcatctg tcttttttat aaaactgttg ctgtgtagaa
     3301 aacgggcgaa ttcctgaata tcggcaatat aagcttttcg agtatgcacc gaaacatttc
     3361 gttccacttc caaataaact tgaaaatctt gaatgagatt ttccatactg ttcacatctt
     3421 atatttaccg aaatcttctg ccgaaagatt ttccagaaat tccttaattt tatcttcttt
     3481 tagtttatcc aaatcactta ttttaattcc gaaatccaca ctgcgcgatt tttcaatgac
     3541 tgtttcatcg acaaatatag gcgcatgcgc gcgcagggcc agagcaatag catcgctggg
     3601 acgagcatca attgtaaaat tttgcccttc tttgcttaaa taaatattag caaaaaaagt
     3661 attgttgcga atatccacga gcacgatttt attcactgtc acatctaaag ttttcaaaca
     3721 ttcgttaaac aaatcatggg tcatgggccg ggaaaaaacg accttttcca gttctgtggc
     3781 tatggcgctg gcttcaaaca aacctatcca aattgggatg gctttactct cttgcaagtc
     3841 tttcaatatt acgatgggcg tattggtaat aggatcgatc gtcaatcccg aaactttcat
     3901 ctcaattaac attaccaacc tccttaatta tcaattattt ttcctcttaa cgaatgtaaa
     3961 taagctttga atatttttac atccaccagt ttgccggtta atcccggatc acctttgaaa
     4021 ttaacaattt tccaggagcg cgttcgtccc atcaaatcat tttcagaatt tttgcttttc
     4081 ccttccacca gtaattcttg cctgcttcct tccaaagctg tgtttttctc tagtgtgtgt
     4141 tgatcctgta attcctgcaa ttgctggagt cttatcaatt tctcatcttc aggaattttc
     4201 ccttctaatt tttgcgccgt agtccctttc ctttcagaat acttgaagga aaaaacagaa
     4261 tcaaacctga tttttcccat catgtcaatg gtttcttgac agtcattttt accctctccc
     4321 ggaaatccga caattatgtc ggaggtaata cttatctgtg ggcaaacttt gcgcaaatga
     4381 tcaacttttt cggcgtattc ctcaacagtg taacccctat tcattaatgc caacactcgg
     4441 tttgatcctg attgcaccgg aaggtgaata tggtcgcata atttttcctc ttcggcaaag
     4501 caattaatca attcttcgga aaggtcacgc ggatgcgaag ttgtaaatct gattctttcg
     4561 atgccggaaa ttttgccgat ttttttaatc aaagccgtaa aattcaatcc attagccagc
     4621 attttaccat aggagttaac attttgtccc agcagtgtaa cttctttaat cccgcaatca
     4681 gccagctttt ttatttcttc gatgatatct ttcggcgacc ggctgatttc cggcccacgc
     4741 aaataaggga caacgcaata ggcacaaaaa ttattacaac cttgcattat tgtgacaaag
     4801 acgctgactg cgcccttttc cggtggagca aatatgccta tggaattgag agatttctga
     4861 aaaccaattt gcgtaatctt t
//
LOCUS       DASVIM010000119         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1582639, whole genome shotgun sequence.
ACCESSION   DASVIM010000119 DASVIM010000000
VERSION     DASVIM010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582639"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VF305_02380"
     CDS             <1..510
                     /locus_tag="VF305_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7416014.1"
                     /translation="KPDDAYYKKAEILARLLAQNGFGVITGGGPGIMEAANKGASEAG
                     GKSVGMNIRLPFEQKPNPYANIHLDYKYFFIRKVMFVKYAIAYVILPGGFGTIDELFE
                     ALTLIQTKRIKSFPLILMGSEYWQGLLDWLKKNMLEENKILPADLELIQVVDEPQEVV
                     KIIKKYIIV"
     gene            530..1885
                     /gene="holA"
                     /locus_tag="VF305_02385"
     CDS             530..1885
                     /gene="holA"
                     /locus_tag="VF305_02385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX7416015.1"
                     /translation="MAMEKIFADLKKGVVAPCYLLYGEEEYLINETLDKILDVIVPAA
                     NRDFGLFYLDGENTDIDSLIKYLLTPSLGGSRKVVVVKNTTVFHSRENLADLIQKIRD
                     NIDEYPAKAAKYFLAFLKIAGFSWEDLQDDGWRKITGDEWRRVVEGDSGEDREKWLPR
                     VLAVSSGIGFSGESSTDNTEKLGEILQKGLAAGNCLILTAETIDKRKKLFKIIDQIGK
                     VIYFGKARSETAQKENLIKEAQKLLENYSKRLSTAAWVALGEKTGWELRTSMAELEKL
                     ISFVGQCPLIEENDVEEVVGKTKESSIFDLTTALGEKNQLAALKALKALLDQGIHHLM
                     ILTMIVREIRFLLQARILVDAGKLPAFKNNMEYGWFQKNVYPALGELKDSIANLSGLL
                     FSQHPFVIYNAWRNCGRFSYPGLVNFLDELLELDRAFKSSATDPQLLLESFLIKACAK
                     A"
     gene            complement(1947..2468)
                     /locus_tag="VF305_02390"
     CDS             complement(1947..2468)
                     /locus_tag="VF305_02390"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX7416016.1"
                     /translation="MHILVDGYNLIRQSDVLRRYERYSLEAGRQALISKLIDYERKKG
                     HKITVVFDGGESGSAQEERDRDGNIDIIYSRHGEKADDVIKRIADQTAEETVVVSSDR
                     DIASYITQRGKTALASVEFEVIINKAISASAEGNFTAEKKDKSHDRNYKKKGPARRLP
                     RAQRHAQTKIKKL"
BASE COUNT          779 a          533 c          591 g          810 t
ORIGIN      
        1 aagcctgatg atgcctacta taaaaaagca gaaattctgg cccggctttt agctcaaaat
       61 ggttttggtg taattaccgg cggcggcccc ggcattatgg aagcggcaaa taaaggcgcc
      121 agtgaagccg gtggaaaatc agtaggcatg aatatccgtt tgccgttcga acaaaagccg
      181 aacccctatg ccaatattca tcttgattac aagtacttct ttatccgtaa agtaatgttt
      241 gtcaaatatg ccatagctta cgtgatttta ccgggcggct tcggcaccat tgatgagctt
      301 tttgaagcgc tgactttaat tcagacaaag agaatcaaaa gctttccttt aatcttaatg
      361 ggcagcgagt attggcaggg cctgttggat tggttgaaaa aaaatatgct tgaggaaaat
      421 aaaatccttc ctgcggacct cgaattgatt caagtagttg acgaacctca agaagttgtg
      481 aagatcatta agaaatatat tatagtttga tcccctgatt tagtccttta tggccatgga
      541 aaaaatcttt gctgatttaa aaaaaggtgt tgttgcgcct tgttatcttc tttatggaga
      601 agaagaatat ctaatcaacg aaactctcga taaaattctg gatgttattg tacctgcggc
      661 caaccgggat ttcggtcttt tttatctgga tggcgaaaat accgacattg atagcctgat
      721 taaatatctc ttaacacctt cccttggggg cagcagaaaa gtggttgtgg tgaaaaatac
      781 cactgttttt cattcccgcg aaaatttagc cgatctaatt caaaagatac gcgacaatat
      841 tgatgaatat ccagctaaag cggcaaaata ttttctagca tttttaaaaa tagctggttt
      901 ttcttgggaa gatttacaag acgacggttg gagaaaaatt accggtgatg agtggcgtag
      961 ggttgtggaa ggtgattcgg gagaagaccg cgaaaaatgg ctgccccgtg ttttagctgt
     1021 ttcatccggt attggattta gcggagaatc cagcaccgat aatacggaga aattaggaga
     1081 aattttacaa aaaggtcttg ccgccggaaa ttgtttaatt cttacggctg aaacaattga
     1141 taaaaggaaa aagcttttta aaattatcga ccaaataggt aaagtaattt acttcggtaa
     1201 agctcgttct gaaacagccc aaaaagaaaa tttaatcaag gaagcccaaa aattactgga
     1261 aaattactct aagcggctgt caacagcggc atgggttgct ttaggggaaa aaaccggttg
     1321 ggaattaaga acttctatgg cggaattgga aaagctcatt tcttttgtcg gccaatgtcc
     1381 cctgatcgaa gaaaatgacg tagaagaagt tgtcggcaag acaaaggaat cctctatatt
     1441 tgacttaaca acggctctcg gcgaaaaaaa tcaactggcg gctctgaaag cgctaaaagc
     1501 tctattggat cagggcatac atcatttgat gattctgaca atgatcgtca gagaaattcg
     1561 atttttgttg caggccagaa ttttagtgga tgcgggaaaa ctgccggcct ttaaaaacaa
     1621 tatggaatat ggatggttcc aaaagaatgt ctaccccgcg ctaggtgaac taaaagattc
     1681 tatagccaac cttagcggcc tcctttttag ccaacatcct tttgttattt ataatgcctg
     1741 gcgtaactgt ggtcgttttt cttatcccgg ccttgttaat ttccttgatg aattactgga
     1801 actcgaccgt gcctttaaat cttcggccac tgacccgcaa cttttactgg agagtttttt
     1861 gattaaagca tgcgctaaag cttgacggct ttccgggaac acgtttcgat ctcttcgggc
     1921 ttccgaagca tgtcccagct atcccatcaa agtttcttaa ttttggtttg agcgtgtctt
     1981 tgtgcacgcg gcagtcttct tgccgggccc ttttttttgt agttgcgatc atgactttta
     2041 tctttttttt ctgccgtaaa gttgccctct gctgatgcag agatagcctt atttatgatt
     2101 acttcaaact ccacggaagc tagggcggtt ttgccgcgtt gggtgatata agaggctata
     2161 tcgcgatcgg aagaaacaac gaccgtttcc tcggcggttt gatctgctat tcttttaatg
     2221 acgtcgtcag ctttttcgcc gtggcgcgag taaataatat cgatgtttcc gtcccggtcg
     2281 cgttcttctt gcgccgatcc actttccccg ccatcgaaaa caacggtaat tttgtgcccc
     2341 ttttttcttt catagtcaat tagtttagat atcaatgcct ggcgtcccgc ctcgaggcta
     2401 taccgctcat aacgccgcaa gacgtcggat tggcggatta aattataacc atcaactaaa
     2461 atatgcataa gttgattctg gcatatccaa gctgcccgtt caataaaatt ctgctatttt
     2521 tttctgttcc ggattttttc ttgacttcgg cgcgcttagt ctgttaggtt tcaacgtgga
     2581 aaaaaagtgc gttaaatatt ttatttaata aaaataaata gatataacag gtgagaaaaa
     2641 taattcaata catcgagaag ccgatgacgt aagttaacaa cgataccccc gaaggcaatt
     2701 tagtataagt aaa
//
LOCUS       DASVIM010000120         8798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1412154, whole genome shotgun sequence.
ACCESSION   DASVIM010000120 DASVIM010000000
VERSION     DASVIM010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412154"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1219)
                     /locus_tag="VF305_02395"
     CDS             complement(155..1219)
                     /locus_tag="VF305_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416017.1"
                     /translation="MRTVRGLVNSFKIFIGFFIILLILSGCATKIKVNMLQPAQYHEA
                     SLIKTIAVLPFSGPEGRAFTAEIEGVLAGIGIDDKRYFTLVDRTSLDKTINEMQLSQS
                     GMVDQKTAVKIGKLVGAQGIYTGVVIRNSYDDSPYYENRSTCVQYEQKRDKDGNLYRG
                     TCISWRQDNANCVRRVAKFAVSPKLVDVTTGRILYSRDLSASANSSGCNDTRPAQSGN
                     ELLERAKESVKKDFRRDIAPYYVTKEISLMDSKEGIESAEAREKLKRGLEYADKGRMD
                     NACELWGEARNIAPNSYALLYNLGVCAESRGDLDAALALYKQADKRLGKPDDDITLAL
                     HRIETAIKNRARLKEQLGSN"
     gene            complement(1235..1963)
                     /locus_tag="VF305_02400"
     CDS             complement(1235..1963)
                     /locus_tag="VF305_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEX7416018.1"
                     /translation="MKKIILFALGYLLLGSLTIALAAEKPRIGVLRFTNITRAYWWSG
                     TTATELQDMLTNELASTKAFQILERKELGSVRSEQQLGESGLVDSSMKLKLGKIKGAK
                     YLVKATVSAFEESTSGTDDGILYKSFSLGGEQKKAYIAVNLKVIDTETGEIIDSRTIE
                     ANTTSGGMRVSGPSGLVPGLSGGLSKQEKTPVGKAIRGCIIEITDYLECSLASAKDDE
                     CHQKYSAKETRRREKTKSSIQLDE"
     gene            complement(2021..3151)
                     /locus_tag="VF305_02405"
     CDS             complement(2021..3151)
                     /locus_tag="VF305_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416019.1"
                     /translation="MKKISLLICFLIILCCWINEAFSQEKCVNAEAEAAIINNDIPSA
                     KLEAIARAKWSAIEQVVGTEIKAQSFVQNFTLVEDVIKTKAGGVVKSYKIIDQKNTTD
                     SVNVKINACVEPASAREAVSQLGLNNSIAIFIPARKPGRSGDEFEETNILSETLIGKL
                     TEQSYKVVDVAPTNALTASVIENAVRSGNSMTVRSMMYKFLSNLIIIGKVDYTISTKK
                     GENIGYGLSMPFNNVTVRLTYRIIAKNNKTGNMEILTAGTEQAKGLANNVEDAAAEAM
                     KELAQNLAPAILDKVAQYIKGNTKKVAIKVNGVNDIDTVVEIKGMLQSIVWVSEVEEK
                     QMGDFIVSYPENTLYLANSIKQKGNYKVVDFSPYSLLLDYQK"
     gene            complement(3238..4617)
                     /gene="mnmE"
                     /locus_tag="VF305_02410"
     CDS             complement(3238..4617)
                     /gene="mnmE"
                     /locus_tag="VF305_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX7416020.1"
                     /translation="MLRQDTIAAIATPLGTAGVGIIRISGPAALEIARRIFQPAPGNC
                     LWESHRLYHGDLVSADGKTNLDEVLISFMRKPRSFTGEDVIEINCHSSPLIIQSILAQ
                     LVELGCRLAQPGEFSERAFFNNRLDLSQAEALAAMVAAKSPKAYALGLAQLKGALSLE
                     IENLRLALIDALALLEAAIDFTEDVSAKETPELPPQIDLALERVQLLLSSYRTAKVYT
                     EGINVVIIGKPNVGKSSLLNTLTGKKKAIVTDIPGTTRDLITDTINIRGIPLNLVDTA
                     GIRKPQNSIEKEGIDLVWEKLSNADVVIIMLDGSKPLTKEDKEIIAKNKNSKILVAIN
                     KSDLPSSWEISTIEKLLPPGQKIIKISAKFGTGLEELKNFIVDISGSADEKDTGNVMI
                     TNMRHKLALEKTLTNILTAKNNILNGMSPEFAAFDLREALDNLDEITGRKINDEILDK
                     IFSSFCIGK"
     gene            complement(4654..5445)
                     /gene="jag"
                     /locus_tag="VF305_02415"
     CDS             complement(4654..5445)
                     /gene="jag"
                     /locus_tag="VF305_02415"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX7416021.1"
                     /translation="MSSKTLEIEDKSIDEAIEKACREFSVPREKLNIEIISEDSGGFF
                     GMLSKKAKIRASLLSLDMDFALTENPQAKETKSEVRIKIEPRIKPETQTAPAVSGSNS
                     SLASQAKELLDGILRRMSLECQVTVNETPEKIILNIEGDENGLLIGKRGQNLDALQYI
                     VNKAINKSDSERKMIIVDSGEYWKRREESLLGLAEKIREKVKKTKKPVSLGHMNAHDR
                     RIIHLALEEDELLTTKSRGEGEYRKIVILPARTGNANQAREKKHE"
     gene            complement(5468..7147)
                     /gene="yidC"
                     /locus_tag="VF305_02420"
     CDS             complement(5468..7147)
                     /gene="yidC"
                     /locus_tag="VF305_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX7416022.1"
                     /translation="MDKRTILAVVLSLAVLLIYQMFIAKPPVPEKVAIPVKEAQQVKK
                     ETATKPVFPQTIPAAKKANAKKAVAAREIRVETSRYIAIFSTEGAALKSFRLKGYEKE
                     CVECADDIYPRIKSFFTGANEPAKPKSNELVELIQLKEDMPYPLAVTFPESNVDVAAD
                     SIFEAKISKLDLINAKEKQRLVFSLTYDGKIKVEKTFTFDPGNYTIGLDVKVYNLTPT
                     PLSQIPRLSWYQYVDPKQEDDSYGHEGPVASVAKSIERQQVKKLNKETIWGPNVLWGG
                     FESKYFIASFIPENPSLTSVILNRDARDIVSVSMKGQKEVIPGGQSGNFDYSLYLGPK
                     NYSLLKTLNVSLEDSIDFGSWLKWLAVPFLLILNFLYAFVHNYGIAIIILTTLVKIIF
                     WPLGNMSYKSMKEMQKIQPKVAELKEKYKNDQAKIGQETMALYRAHKVNPLSGCLPMI
                     IQIPVFFGLYKALLYSIELRHSPFFWWIQDLSAKDPYYITPIIMGVTQFIQQKMTPAM
                     GDPMQAKIMLLMPVIFTFFFLNFPAGLVIYWLFNNILSIGQQFYINRKLAS"
     gene            complement(7284..7511)
                     /gene="yidD"
                     /locus_tag="VF305_02425"
     CDS             complement(7284..7511)
                     /gene="yidD"
                     /locus_tag="VF305_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX7416023.1"
                     /translation="MLRTILVNFFVSIIRSYQICLSPFFFAPCCRFYPSCSEYAISAI
                     KLHGPTKGSFMGIKRILRCHPLHPGGYDPVK"
     gene            complement(7515..7862)
                     /gene="rnpA"
                     /locus_tag="VF305_02430"
     CDS             complement(7515..7862)
                     /gene="rnpA"
                     /locus_tag="VF305_02430"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX7416024.1"
                     /translation="MKEQSYRKLERITNRSRFRAIYQQGVWRSSQHFTIITCSNIQGV
                     KRLGITVSKKVGNAVKRNRIKRLIREFFRLNKALFPAEYDVVVMAKRNMPPLTYQEAC
                     KELTELFTRKANI"
     gene            complement(7905..8042)
                     /gene="rpmH"
                     /locus_tag="VF305_02435"
     CDS             complement(7905..8042)
                     /gene="rpmH"
                     /locus_tag="VF305_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX7416025.1"
                     /translation="MKKTLTNKTNTKRKRTHGFLVRMASKSGRQVLSRRRAKGRKRLA
                     V"
     gene            complement(8132..>8798)
                     /locus_tag="VF305_02440"
     CDS             complement(8132..>8798)
                     /locus_tag="VF305_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7416026.1"
                     /translation="SFVGSLYAYGFNPYELQTIAFAIKKDDVADYIIPDNGFIKGERL
                     ENFINSKVKYTTIEKFRIPFYAVATNIQNGEEMVFGRGNTGKAVRASCSIPGIFNPVV
                     IGDKYYVDGGLVNPLAADVARRYGADVVIAVDISSSFETAAPTGTVETIMQAIDIMYS
                     KMAAIQFKNADVVIKPKVGHIGASDFTKRHEAIMEGERAAVEAMPAINQIIAKLKQEG
                     RLP"
BASE COUNT         2291 a         1970 c         1815 g         2722 t
ORIGIN      
        1 taagcagcgt tcccaaacca aaaccggaaa acagcgttaa cgcggatacc agtaaagcga
       61 ccaagcatat aattatataa tccattgtat caatctatta tagttatatt aagaaatagc
      121 tgctgtttga tattgccttg actaaaaaag atcttcagtt gcttcctaat tgctctttga
      181 gtctagcgcg gtttttaatg gcggtttcga tccggtggag agctaacgtt atgtcgtcat
      241 cgggcttgcc taagcgctta tcggcttgtt tgtaaagcgc caaggcggca tcgagatcgc
      301 cgcggctttc cgcgcaaaca cccaggttat aaagcaaggc ataagaatta ggggcgatat
      361 tgcgcgcttc tccccatagt tcgcaggcat tatccatcct gcccttatcc gcatattcca
      421 aaccacgttt aagtttttct ctagcttccg ctgattcgat gccttctttg gaatccatta
      481 aactgatttc cttggtgaca tagtatgggg caatatcgcg ccggaaatct ttcttgaccg
      541 attccttggc cctttctaaa agttcatttc cgctttgtgc cggccgcgta tcattgcagc
      601 cggaagaatt tgccgaagcg gaaagatcac gggaataaag aattctgccg gtggtaacat
      661 cgaccaattt aggcgaaacg gcgaatttgg caacacgcct gacacaatta gcgttatcct
      721 gccgccacga aatacaggtg ccacgatata aattgccatc cttgtctctt ttttgttcgt
      781 actgcacaca agttgaacga ttttcataat aggggctatc gtcatagcta ttccggatga
      841 caacgccggt ataaatgccc tgggcaccga caagtttacc gatctttacc gctgttttct
      901 ggtccaccat gccgctttga gaaagctgca tttcgttgat ggttttatca agagaggtac
      961 ggtcaacaag agtaaagtaa cgtttatcat cgataccgat gcccgctaat accccttcaa
     1021 tttcggcagt gaatgccctg ccttcagggc cgctgaaagg cagtacagct atggttttta
     1081 tcagcgacgc ttcgtgatat tgcgcgggct ggagcatatt gactttgatt tttgtggcac
     1141 aaccggaaag tattaataaa ataataaaga aaccaataaa aatcttaaaa ctattaacta
     1201 atccgcgcac ggtacgcatt ttcccctccc tttattattc gtcgagttga atggacgatt
     1261 tggttttttc ccttctcctg gtttcttttg ccgaatattt ttgatggcat tcatcatctt
     1321 tggcgctggc caaagaacat tccagatagt cggttatttc aataatgcag ccgcgaatgg
     1381 ctttgcccac tggagtcttt tcctgcttac ttaagccgcc ggaaagtccg ggaacaaggc
     1441 cggacgggcc gctgactctc ataccgccgg aagttgtgtt tgcttcaatt gtgcgggaat
     1501 cgataatttc accagtttcc gtatcgatta ctttcagatt aacggcaata taggctttct
     1561 tttgctctcc ccccaaagaa aaactcttgt atagaatacc gtcgtcggta ccgctggtgc
     1621 tttcttcaaa agcggaaaca gtcgccttca ccaagtattt cgctccttta atctttccca
     1681 gcttcaattt catggacgag tcaaccaaac ccgattcgcc gagttgttgt tcgctccgta
     1741 ctgaaccaag ttctttacgc tccagtattt gaaaagcttt ggtggaagcc agttcattgg
     1801 tcagcatatc ttgcaattca gtagcggtgg tacccgacca ccagtaggca cgggtaatgt
     1861 tggtgaagcg taaaactccg atgcgcggtt tttccgcggc taacgctatc gttaaactac
     1921 ctaaaagaag atatcccaaa gcaaataaaa taattttttt catgatctgt gctcttctta
     1981 aaacacctta taccctctaa tcagaaaaaa ctgggccacc ttatttttga taatctaaaa
     2041 gaagagaata aggcgaaaaa tcaacaactt tatagtttcc tttttgtttt atactgttgg
     2101 ctaagtaaag cgtgttttcg ggatagctga cgatgaaatc acccatttgc ttttcttcga
     2161 cttcagaaac ccagacaata ctttgcagca ttcctttaat ttccacgacc gtatctatat
     2221 catttacgcc attaacctta atagcgactt tcttcgtatt gcctttgatg tactgggcta
     2281 ctttatccaa aattgcaggc gccagatttt gcgccaattc tttcattgct tctgcggctg
     2341 catcttccac gttatttgct aatcccttag cctgctcggt acccgctgtc aagatttcca
     2401 tattgccggt cttattgttt ttggcaataa tccggtaagt cagacgcact gtcacattat
     2461 taaaaggcat ggaaaggccg tagccgatat tttctccttt tttagtggaa atcgtgtaat
     2521 caactttacc gatgataatt aagttggata aaaatttata catcatgctg cggacagtca
     2581 tgctattgcc gctgcgcacg gcattttcga ttactgaggc ggtaagcgcg ttagtggggg
     2641 caacatcgac aaccttatag ctttgttcag tgagcttacc gataagtgtt tctgaaagga
     2701 tatttgtttc ttcaaattca tcgccgctgc ggccaggctt gcgtgccgga ataaaaatag
     2761 caatagaatt atttaatcct aactgggaaa cggcctccct ggcgctggcc ggttccacac
     2821 aagcgttaat cttgacattg acactatctg tggtattttt ctgatctatt attttataac
     2881 ttttaactac accgcccgct tttgttttaa taacatcttc gaccagagta aagttttgca
     2941 caaagctttg cgctttgatt tcggtgccaa cgacttgttc gattgccgac catttggcgc
     3001 gggcaatggc ttccaactta gctgaaggaa tatcgttatt aataatagct gcttccgcct
     3061 cggcattaac gcatttttcc tgactaaacg cttcgtttat ccaacaacaa agaatgatca
     3121 aaaagcaaat taacaaggaa atttttttca taatattacc tcattttatg tccttaacta
     3181 taaaaaccat accaaaaata tgatgtaata ctagcagaaa gttacgcgag attcaattta
     3241 cttgccgatg cagaaactag agaatatctt atccaatatt tcatcattaa tttttctgcc
     3301 ggtgatttca tccagattat ctaaggcctc gcgcaggtca aaagcggcaa attccggcga
     3361 catgccgttt aaaatattat tttttgccgt cagaatattc gtaagtgttt tttccagcgc
     3421 cagtttatgg cgcatattgg taatcatcac gttaccggta tctttttcat cggcgctgcc
     3481 tgatatatct acaatgaaat tttttaattc ttccagtccc gtgccgaatt tggccgagat
     3541 tttaataatt ttttgccctg gaggaagtag cttttcaatt gtgcttattt cccaagaaga
     3601 cggcagatca cttttattga tcgcgacaag aattttgcta ttcttattct tggcaataat
     3661 ttctttgtct tccttcgtta aaggtttgct gccgtccaac attataatca ctacatcagc
     3721 attggacagt ttttcccaga ccagatcgat tccttctttt tcgatggagt tttgcggttt
     3781 tctaattccg gcagtatcca ccagatttaa aggaataccg cgaatgttga tggtatccgt
     3841 gattaaatcg cgcgttgtac cggggatatc ggtaacaatc gcctttttct ttcctgtcag
     3901 cgtattgagt aaactcgatt tgcccacatt gggcttgccg atgatgacga cgttgatgcc
     3961 ttcagtatag acttttgccg tgcgatacga tgacaaaagt aattgcacgc gttctaacgc
     4021 cagatcaatt tgcggcggca attccggagt ttctttagcg gatacatctt cggtaaaatc
     4081 aatggccgcc tccagtaagg ccagcgcatc gatcaaggca agtcgcaggt tttctatttc
     4141 caggctcagt gctcccttta actgagctag accaagggca taagcttttg gagatttggc
     4201 ggcaaccatt gcagcgagtg cttccgcttg cgataaatca aggcggttat tgaaaaaagc
     4261 gcgctcagag aattcgccgg gctgggcaag gcggcagccc aattctacca attgtgccaa
     4321 aatagattgt ataatgagcg ggctgctatg gcagtttatt tctataacat cttcccccgt
     4381 gaaagagcgt ggtttgcgca tgaaagatat caaaacttca tcgaggttcg ttttaccgtc
     4441 ggcagagacg agatcgccat gatataaacg atggctttcc cacaaacaat ttcctggggc
     4501 gggctgaaaa atgcggcgcg cgatttcgag agctgcgggt ccgctgatcc ggattatccc
     4561 gactcctgcc gtgcccaaag gagtggcaat agcggctata gtgtcttggc gaagcacagt
     4621 agggcacttc ccctttatat tagaccaaaa aaactattca tgcttttttt ctctggcctg
     4681 gttggcattg cctgttctcg cgggaagaat aacgattttt ctgtattcac cttcgccgcg
     4741 gcttttcgtg gttagcaatt catcttcttc cagcgccaaa tggataatgc gccggtcatg
     4801 ggcgttcatg tggcccagtg ataccggttt cttggtcttt tttacctttt cccttatttt
     4861 ttccgctaaa cccagcagag attcttctct tcttttccag tactctcctg aatccactat
     4921 gatcattttg cgttcgctat cggatttatt gattgcttta ttcactatgt actgtaaggc
     4981 gtcaaggttt tgtccacgtt tgccgataag caaaccgttt tcatcacctt caatgttgag
     5041 aattattttt tccggcgttt cattgacagt cacctggcat tccaacgaca ttcttcgtaa
     5101 aatgccgtcc aataactctt ttgcctgaga ggccagtgaa gagttggaac cggaaactgc
     5161 tggcgctgtt tgcgtttccg gtttgatcct tggttcgatt ttaattctta cttcgctttt
     5221 tgtttccttt gcttgaggat tttcagttaa agcaaaatcc atatccaatg agagcaagga
     5281 agctctgatt tttgcttttt tggaaagcat cccaaaaaac ccgccagagt cttcagaaat
     5341 aatttcaata tttaattttt cccgcggcac gctaaattcg cggcaagctt tttcaatggc
     5401 ttcatcaatt gatttatctt ctatttctaa agttttcgaa ctcatctata aacctcatat
     5461 caatatatca actagctaac tttctattaa tgtaaaattg ttggccgatg cttaaaatat
     5521 tattaaatag ccagtaaatg accagtcccg cggggaaatt taagaagaaa aaagtaaaaa
     5581 ttacgggcat taataacata atctttgcct gcataggatc gcccatagca ggagtcattt
     5641 tctgttgtat aaattgcgtt acgcccatga tgataggagt aatgtaataa gggtctttgg
     5701 ccgacaaatc ctgaatccac caaaaaaacg gtgaatggcg cagttcaatg gaataaagca
     5761 gagctttgta gaggccgaaa aatacaggaa tttgaattat catcggcaag catccgctaa
     5821 gcgggttaac tttatgagcg cggtaaagcg ccatggtttc ctggccgatc ttggcctgat
     5881 catttttgta tttctctttt aactcagcta ccttaggctg aattttttgc atttccttca
     5941 tggacttgta actcatgttg cccaatggcc agaagattat tttaaccaat gtggttaaaa
     6001 tgataatcgc aattccgtag ttatggacaa acgcgtaaag aaaattaagg attaaaagga
     6061 acggcacagc caaccatttc aaccagctgc caaaatcaat ggaatcttct aaactcacat
     6121 tgagtgtctt gagcaaacta taattcttcg gaccgagata cagggaataa tcaaaattac
     6181 cactttgtcc gccggggatc acttcttttt ggcccttcat gctgactgag actatgtcgc
     6241 gggcgtcacg attaaggatg acgctggtta atgagggatt ttccggaata aaggaagcga
     6301 taaaatattt actttcaaag ccgccccaca atacattggg accccatatt gtttctttgt
     6361 tcagcttttt gacttgctgc ctttcaatgc ttttggcaac ggaagcaacc ggaccttcat
     6421 ggccataact gtcatcttct tgtttgggat cgacatactg ataccagcta agccgcggta
     6481 tctggctcag cggtgttgga gtaaggttat atactttgac gtccagcccg attgtgtaat
     6541 taccgggatc aaaagtgaaa gtcttttcaa ctttaatttt gccatcataa gtcagggaaa
     6601 aaactaatcg ttgtttttct ttggcattga ttaagtccag tttagatatt ttggcttcaa
     6661 aaatagaatc ggcagcaacg tcaacgttgg attccggaaa agtgacggcc agcggataag
     6721 gcatatcctc tttaagttga ataagttcta ccagttcatt gctcttgggt ttggccggtt
     6781 cattagcgcc ggtaaagaag cttttaatgc gggggtaaat atcatcagca cattcaacac
     6841 attctttttc atagcctttg agccgaaatg atttcaaggc agcgccttcg gtagaaaaga
     6901 tagcgatgta acgggaagtt tccaccctga tttctctggc tgcaactgcc tttttagcgt
     6961 tagccttttt tgctgccggt attgtttgcg gaaaaaccgg ttttgtagcc gtctcttttt
     7021 taacctgctg tgcttccttg acaggaatag ccaccttctc cggcaccggt ggtttggcaa
     7081 taaacatctg atatattaaa agtacggcaa gtgataggac tacagcaagt attgtccttt
     7141 tatccattga aaacctccct attatttttt taccccgtta gagaaagccc ccaactcctg
     7201 ttggatggtt aatgtcttgt tagacagatg gcgatttaat gccgccatct gaaatcgcaa
     7261 agcccccgtt actctaacgg ggtttactta acgggatcgt aaccgccggg atgcagaggg
     7321 tggcagcgca gaatcctttt tatgcccatg aatgaccctt ttgtcgggcc gtgcaattta
     7381 attgccgaaa tggcatattc ggagcatgaa gggtagaatc ggcaacatgg agcaaaaaag
     7441 aaaggtgaca aacaaatttg gtaggaacga attatggaaa caaaaaaatt taccaaaatt
     7501 gttctgagca taaattatat attggctttc cgcgtgaaga gttctgtcaa ttctttgcat
     7561 gcttcttgat aagtaagggg cggcatattt cttttagcca tgacgacgac atcatactcg
     7621 gcgggaaata aggccttatt gaggcggaaa aattctctga tcagccgttt gattctattt
     7681 ctttttacgg cattccctac ttttttggat acggttattc ccagcctttt aaccccttgg
     7741 atattgctgc aagttatgat tgtaaaatgt tgcgaacttc tccacacccc ctgttggtaa
     7801 atggctctga acctgcttct attagtgatc ctttctaatt tccggtaaga ttgttctttc
     7861 atgcataccg accgaccatc ccttaaggca ctaaaaaccc aaatttatac ggctaaacgt
     7921 tttctacctt tggcccttct gcggcttaaa acttgtctgc cggattttga agccatccgg
     7981 acaagaaaac cgtgggtcct ttttcttttg gtattcgtct tattggtcaa agtctttttc
     8041 ataagtaaaa cccctaaaag ttatagggtc atgcgtaaat cacagacaca tttatttgtc
     8101 aaggcaaaat tggcgagtaa ccgcaactct cttacggcag tcgtccttct tgctttagtt
     8161 tagcgataat ttggttaatt gccggcatgg cttccactgc cgccctttcg ccttccatga
     8221 tggcttcatg tcgtttggtg aaatcgctcg cgcctatgtg ccctactttc ggcttgatta
     8281 ccacatcggc gtttttaaat tgaattgccg ccattttgct gtacattata tcaatcgcct
     8341 gcatgatcgt ttcgaccgtg ccggttggtg cggcggtctc aaaactcgat gatatatcca
     8401 cagctatcac cacgtccgca ccgtacctgc gggctacatc cgcagccaaa ggatttacca
     8461 acccgccatc aacataatat ttatcgccaa taaccacagg attgaaaatt cctggaatcg
     8521 agcaactggc gcgaacagcc ttgccggtat tgccgcgacc gaagaccatt tcctctccgt
     8581 tttggatatt tgtcgctacg gcataaaaag gaatcctgaa tttttctatt gtcgtatatt
     8641 ttactttaga attgatgaag ttttcgagtc tttctccttt gataaaacca ttatcgggta
     8701 tgatataatc ggcaacatcg tcttttttaa tggcaaaagc aatagtctgc aattcgtagg
     8761 gattgaaacc gtaagcatag aggctgccga caaaggaa
//
LOCUS       DASVIM010000121         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_899576, whole genome shotgun sequence.
ACCESSION   DASVIM010000121 DASVIM010000000
VERSION     DASVIM010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899576"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VF305_02445"
     CDS             <1..413
                     /locus_tag="VF305_02445"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416027.1"
                     /translation="EKLNSYCFSVSSKNELLVGGRKICGSAQVRKKGGFLQQGSLLLS
                     FDPIKTAALLLPTRTPEQLQKLKKSVAALNEELLTPVDVQEACFKLKEGFINELGIEL
                     VEESLTLAEETLKNKLMEKYSNARWNIEGKNIFN"
     gene            431..1486
                     /gene="hisC"
                     /locus_tag="VF305_02450"
     CDS             431..1486
                     /gene="hisC"
                     /locus_tag="VF305_02450"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX7416028.1"
                     /translation="MRDIVNKEIFKQSGYKAPELTGAIKMDANENPFSLQEPLKKRFL
                     EEICKVNLNRYPEAGAPKLRDRFAQYYGVDKDMLMLGNGSDELIQILCMTFQGKTCGV
                     LVPIPTFVMYKIIAVNTGHKVMEVPLDKNFDLDIDAFIGKIRATFPSLIFLSYPNSPI
                     GNLFNRDKIETIIKKSAGVVVIDEAYANFSGQTLLPLLKKYDNLVFLKTLSKLGLASM
                     RLGFLIGNRDIIAQLDKVRLPYNVNSLSQVAANFFLNYQNEFSKQVTEIVKERGKLYS
                     GMKKITGIKPYPSRANFIFFSCAFDSDRIYSRLEAAGIIVKNLNTNLTKNCMRVTVGN
                     PKENAAFLNVLKSVISE"
     gene            1632..1823
                     /gene="rpsU"
                     /locus_tag="VF305_02455"
     CDS             1632..1823
                     /gene="rpsU"
                     /locus_tag="VF305_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX7416029.1"
                     /translation="MEVKVIDNDVEKALKILKNKLSKSGLFKELKVRRAYEKPSVKKK
                     RKTIEARRRLAKVQRRRSN"
     gene            1881..>2165
                     /locus_tag="VF305_02460"
     CDS             1881..>2165
                     /locus_tag="VF305_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7416030.1"
                     /translation="MEEKQYLIDSLSTEESWRMFRIMAEFVDAIETLSNVHNAVSIFG
                     SARVKPDDAYYKKAEILARLLAQNGFGVITGGGPGIMEAANKGASEAGGKS"
BASE COUNT          708 a          352 c          461 g          644 t
ORIGIN      
        1 aggaaaaatt aaactcctac tgtttttcgg tttcttctaa aaatgagctt ttagtcggag
       61 gacgtaagat ttgcggtagc gcgcaagtga gaaaaaaagg tggatttttg cagcagggtt
      121 cattactttt gtcttttgat ccgataaaaa cagctgcttt gttactgccc actcgtacgc
      181 ctgagcaatt gcaaaaacta aaaaaatcag ttgccgcgct caatgaagag cttcttacgc
      241 cggttgatgt gcaagaagct tgttttaaac taaaagaagg atttattaat gaactgggta
      301 tagaattggt ggaagaatct ctaacactgg cggaagaaac tctgaaaaat aaattaatgg
      361 aaaaatacag taatgctcgt tggaatatag aaggtaaaaa catttttaat taggttttat
      421 tgccatgatt ttgagggaca tagtaaataa agaaatattt aaacaaagcg gttataaagc
      481 gccggagttg acgggcgcga taaaaatgga cgccaatgag aatcctttca gtttacagga
      541 gcctttaaag aaaaggttct tggaagaaat ttgtaaagta aatttaaacc gctatcctga
      601 agccggcgct cctaaattac gcgatagatt tgctcagtat tacggtgtcg ataaggacat
      661 gctcatgttg ggcaacggat cggatgaact aattcaaatc ctttgtatga cgttccaagg
      721 taagacctgt ggcgtgctgg ttcctattcc tacttttgtc atgtacaaaa tcattgcggt
      781 taatacaggc cataaagtga tggaggtacc tttagataaa aacttcgatc ttgatattga
      841 tgcatttatc ggcaaaatta gggcaacctt cccttctttg atatttttaa gttatcccaa
      901 tagtcctatc ggtaatcttt ttaatcggga caaaatagaa acgataatta agaaatctgc
      961 gggagtagtt gtcatcgacg aagcgtatgc caatttttcc ggtcagactt tgttgcccct
     1021 gctgaaaaaa tacgataatt tggttttttt gaaaacgctc tccaaattgg gattggcctc
     1081 aatgcggctt ggtttcttaa tcggcaatag agatattatc gctcaattgg ataaagtaag
     1141 attaccttat aacgtgaatt cattgtcaca agtagccgct aatttttttc ttaattatca
     1201 aaacgaattt tccaaacaag ttaccgaaat agtcaaagag cgtggaaaac tttactcggg
     1261 catgaaaaaa attaccggaa ttaagcccta tccatcgcgt gcgaatttta ttttttttag
     1321 ttgcgctttt gattccgatc gcatatatag cagattagag gcggctggta ttatcgttaa
     1381 aaatttaaat acaaacctga cgaaaaactg catgagagtt acagtaggaa atcctaaaga
     1441 aaacgcagct tttttaaatg ttttaaaaag tgttatttct gagtaaggag cgtgatttaa
     1501 aattaattga aaattgtgag ggtagtttac ggcacgataa ttattggact ttgccgtact
     1561 gccctttttt tatttctaaa cgattttagg taaaataatt gattaattat aagggataag
     1621 gaggaattaa cttggaagta aaggtaattg acaatgatgt ggaaaaagct ttaaaaatat
     1681 tgaaaaataa actgtcgaaa agcggcttat tcaaggaatt gaaagttcgc cgtgcttatg
     1741 aaaagccttc cgtaaagaaa aagagaaaaa caattgaggc acgccgcaga ctggccaagg
     1801 ttcaaagacg ccgcagtaat taaaggaaaa ttttttattg cattaatcta catattgcag
     1861 ttggggaaca atgttgtcac atggaagaaa aacaatactt aattgattca ctgtccactg
     1921 aagaatcttg gcggatgttt cgtattatgg cggaatttgt tgacgccatc gaaactcttt
     1981 ccaacgtgca taatgctgtc tctattttcg gttcagccag agtaaagcct gatgatgcct
     2041 actataaaaa agcagaaatt ctggcccggc ttttagctca aaatggtttt ggtgtaatta
     2101 ccggcggcgg ccccggcatt atggaagcgg caaataaagg cgccagtgaa gccggtggaa
     2161 aatca
//
LOCUS       DASVIM010000122         4680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1499357, whole genome shotgun sequence.
ACCESSION   DASVIM010000122 DASVIM010000000
VERSION     DASVIM010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499357"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VF305_02465"
     CDS             <1..223
                     /locus_tag="VF305_02465"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX7416031.1"
                     /translation="PASMRVLSACQPIYEELDGWKEDILYAKEMSELPTNARKYLKRL
                     EELAEAKIVLVSVGAGREETIILEDPFHN"
     gene            297..371
                     /locus_tag="VF305_02470"
     tRNA            297..371
                     /locus_tag="VF305_02470"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:329..331,aa:Lys,seq:ttt)
     gene            474..899
                     /locus_tag="VF305_02475"
     CDS             474..899
                     /locus_tag="VF305_02475"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEX7416032.1"
                     /translation="MDEKIRNALLKQVAVEPFAKIFGLQLIDIQEGYAKVEMIFTQDL
                     ENMFGMAHGGALFALIDEAFEVASNSHGTMAVALNMSVNYLASPDKGSKLTAEAKEIN
                     KAKKTAAYDIIVTDDKNNLIAACQALVYRMEKPLPFLED"
     gene            977..1594
                     /locus_tag="VF305_02480"
     CDS             977..1594
                     /locus_tag="VF305_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416033.1"
                     /translation="MKKQLFNVMYLISPAIFVFLCACQTTSSNLSSDPNNYNVNLHLI
                     DYFPKLDAAKYQQFKGKKICMSNIRNNAGNTTNFSYYSKDLKVQYQLSHASNTPIQFI
                     PSYFWYAYQKAFQYAGIEASADCSSANLPELWIIFQSFNDEELQLKITVLKNRETLYE
                     KDLIITMAASSSRNSILLKIRAYEMIDLTITTILDDPGLQAILLQ"
     gene            complement(1718..3583)
                     /locus_tag="VF305_02485"
     CDS             complement(1718..3583)
                     /locus_tag="VF305_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX7416034.1"
                     /translation="MALNPLVDSRDVRFVLFEVLQADKLSKYDKFSGFDKDTYEATLE
                     LAEQLAVKRVYPANAEADKTGAKYDPETKSVTIPEGYKSAMKLYKESGFSGLGNDPEW
                     GGVGMPEVICRAVIDYFSAAAVAFIIYDTLKGGASRLIEKFGSEDLKKTYMEKMVSGQ
                     WGGTMCLTESGAGSDVGALKTKAVKQKDGTYLITGQKTFISGGDNDLYENIIHPVLAR
                     VEGDPPGTPGISIFIVPKYHVNSDGTIGDRNDIITTGIEHKMGIKGSATCSLSFGDND
                     KCVGYILGAERQGMKIMFQMMNEARLDVSQQALGIAGSAYMHAVTYAKNRIQGSDPAK
                     KGDTSSAPIVKHPDVKRMLLWMKAQVEAMRMLTLFAAYSADISRMEKGDAAKEASALL
                     DFLIPLCKAGNTDLVWLITSEAMQVYGGYGYCSEYPVEQLARDCKILAIYEGTNGIQS
                     IDLTMRKLLMNPEMYNYKVFKKRIQETIDKAKGIVDEKYIAAVRDGVGKMDQTIQKLN
                     EYKEQKKMLDILAIATPLQQAMRMLAHAWMHLWSLTLAIPKLKAIAGDISGEKVVNIV
                     KDNSEAAYYYGKVLSGRFYLGAEFKHYSGFLDYITSGEGAVVESFEDIFTGALAQ"
     gene            complement(3730..>4680)
                     /gene="nrdD"
                     /locus_tag="VF305_02490"
     CDS             complement(3730..>4680)
                     /gene="nrdD"
                     /locus_tag="VF305_02490"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEX7416035.1"
                     /translation="DAAVSGELDKPWLMRSAAIQSVTLNLPRLGYKANGDEAKLFSLL
                     KDIAATAVKAHIQKKDFIEKLLSYAEQGPLAVLAMNNDGFPFLRMNRAYYIIGIIGLN
                     ELAQIQLGSQLHQSGEALEFGLRVVEYLQRETKHLSKELGMKFVLEQTPAETTAYRFA
                     RLDLKYFSPAAGHYVKGDIAEGAIYYTNSTHLNVSADVTPLQRVIQEGLFHKYLEGEV
                     ITHLQLGGSNPGKEALSRFVREVFYNSGNRQIDFAPEFTSCLSCDKTAKGLNKNCIYC
                     GSTEVEGIARLTKYFSKISSWNKGKLAELKYRKVSDNFDK"
BASE COUNT         1313 a         1121 c          900 g         1346 t
ORIGIN      
        1 tcctgccagt atgcgcgtac tttcagcgtg tcagccaatt tatgaagaat tagacggctg
       61 gaaggaagat attctctatg cgaaggaaat gagcgaactg cccaccaatg ccaggaaata
      121 tctgaagcgg ctggaagaat tagcggaagc taaaattgtt cttgtatccg taggcgcggg
      181 ccgggaggaa acaataattc tggaagatcc tttccataat taaaatattc tagtttcttc
      241 ttgacacatt cgaaacttgc tgttaaaaga ccacgtttaa aaattatcca taaagtgggc
      301 tgttagctca gcggtagagc agcggacttt taatccgttg gccgagggtt caaatccctc
      361 acggcccacc atttacaaag gcttccgcga ggaagccttt tttttgactc caaccatcac
      421 aataatgtat actctcctca ataaaatttc ctgattttat tgaggagaca atcatggatg
      481 aaaagattag gaacgctcta ctcaaacaag tagcagtcga gcctttcgcc aaaatatttg
      541 ggttacaatt aattgacata caagaaggat atgccaaggt ggaaatgatc tttactcaag
      601 atttggaaaa tatgttcggt atggctcacg gtggagcttt atttgcactt atcgatgaag
      661 cttttgaagt cgcctccaac tctcacggca ctatggctgt ggctttaaac atgagcgtaa
      721 attatctcgc ctcgccggac aaaggttcga aattgactgc cgaagcaaaa gaaatcaata
      781 aagcgaagaa aaccgccgcc tatgatatta tcgtgactga cgataaaaat aatctcattg
      841 ccgcctgtca agcacttgtt tatcgaatgg aaaagccgct tcctttcctc gaagattaag
      901 ccagaagccg tggcattagt ttttatgttt atctataata tatatgcgaa acatcttccc
      961 aagggagggt aaaaaaatga agaaacaatt gttcaatgta atgtatttaa tttccccggc
     1021 tatttttgtt tttctttgcg cctgccagac gacatccagc aatttgagca gtgatcccaa
     1081 taactacaat gtaaatctgc acctaatcga ttacttccca aaattagacg cggcaaaata
     1141 tcaacaattt aaagggaaaa agatctgtat gtccaacatc agaaacaatg ccggcaacac
     1201 aacaaatttc tcttattata gtaaagactt aaaagtccaa tatcaacttt cccatgcgtc
     1261 aaatactccg atacagttta ttcccagcta cttttggtac gcatatcaaa aggcattcca
     1321 gtatgcggga atcgaagcct cagccgattg ttcatctgct aacttgccgg aattgtggat
     1381 tatttttcaa tcattcaatg atgaggagtt acagctaaaa attactgtac ttaaaaaccg
     1441 cgagacacta tacgaaaaag acctgataat tactatggct gcctcttcca gccgtaattc
     1501 aatcttgcta aaaattcggg cgtacgagat gattgatttg acgattacga cgatactcga
     1561 cgatcccgga ttgcaggcga ttcttttgca atagtctgcc caagaatttg tctcaataga
     1621 aaaaatgacc cctgcagcaa atacgctgcc ggggtcattt tcaatacaaa aacttttctt
     1681 tagatagtac cagtcgtgaa caataaatta aaaaatttta ttgtgccaat gctccggtaa
     1741 atatatcctc aaaggattca actacagctc cttctcccga tgttatataa tccaggaagc
     1801 cggaataatg tttgaactca gcgcctagat aaaatctgcc ggacaatact tttccataat
     1861 agtatgccgc ctcagaatta tccttgacaa tatttacaac tttttctcct gatatatcgc
     1921 cggcaatcgc ttttagtttc ggaatagcta atgtcaacga ccacaaatgc atccaggcgt
     1981 gggccagcat cctcatggct tgctgcaagg gagtggcaat cgccaaaata tccagcattt
     2041 ttttctgttc tttatactcg ttgagttttt gtatcgtttg gtccattttt cccacgccat
     2101 cacgaacagc ggctatatat ttttcgtcaa cgatgccttt ggccttatct atggtttcct
     2161 gaatcctctt tttgaagact ttataattat acatttccgg attcatcagc agttttctca
     2221 tggtcagatc tatggattgg atgccgtttg tcccctcgta aatggccaaa atcttacaat
     2281 cgcgcgccaa ttgttcgaca ggatattccg agcaatatcc gtaaccgccg taaacctgca
     2341 tggcttcaga agttatcagc caaaccaagt cagtgtttcc cgctttgcac aagggaataa
     2401 gaaaatcgag cagcgctgat gcttctttgg cagcatcacc cttttccatc cggctgatat
     2461 cggctgaata agcggcaaac agagtaagca ttctcatcgc ttccacctgc gccttcatcc
     2521 atagcaacat tcttttcacg tcaggatgtt ttacaatcgg cgctgaagat gtatctccct
     2581 ttttcgcggg atcggacccc tgtattctgt tttttgcata ggttaccgca tgcatatatg
     2641 cggaaccagc aatacctagc gcctgctgcg acacatccag acgcgcttcg ttcatcatct
     2701 ggaacataat cttcattccc tgccgttctg ctcctaaaat atagcccaca catttatcgt
     2761 tatcgccaaa acttaatgaa caagtagccg accctttaat tcccatcttg tgctcaatgc
     2821 ctgtggttat aatatcattg cgatcgccaa tggttccgtc ggaattgaca tgatatttag
     2881 gcacaatgaa tatcgatatt cccggtgttc ccggaggatc cccttcaact ctggccagaa
     2941 ccgggtgaat aatattctca tatagatcgt tatcaccgcc ggagatgaat gtcttctgtc
     3001 cggtaataag ataagtgccg tctttttgct tgaccgcttt tgtctttaaa gcgccgacat
     3061 cggaaccggc accggactcg gtaagacaca tagtcccacc ccattgaccg gataccatct
     3121 tctccatgta tgtcttcttc aagtcttcag atccgaattt ttcgataagc ctggatgccc
     3181 cacccttcaa cgtatcgtaa atgataaaag caactgcggc ggcggaaaaa taatctatta
     3241 cggcgcggca tatgacctcg ggcatgccca cgccgcccca ttcaggatca tttcccagac
     3301 cggaaaagcc ggattctttg taaagcttca ttgccgactt atatccctca ggaatagtaa
     3361 ccgactttgt ttcgggatca tacttagcac cggtcttatc ggcttcggca ttggcgggat
     3421 aaacccgctt gacggcaagt tgttccgcca gctccagcgt ggcctcatat gtatccttat
     3481 cgaagccgga aaatttatcg tacttactaa gcttgtccgc ttgcaatacc tcaaataaaa
     3541 caaatcttac atctcgtgaa tcaaccaacg gatttaatgc cataacacac tcctcaattt
     3601 atattttttt gcagcattgc catgccgata acatcaccag cctctataat catgaggctg
     3661 cggctaaaat aatccggcaa tttatttctt ttctgtcctg ccgcaagttt ttatcgaacg
     3721 tatactactc tacttatcaa aattatcact tacttttcta tacttgagtt cggcaagttt
     3781 tcctttattc caactggata ttttgctgaa atattttgtc agtcgagcga tgccttccac
     3841 ttccgtagat ccgcaataaa tacaattttt attcaagccc ttcgcagttt tatcgcagga
     3901 aaggcaagat gtgaattccg gggcaaaatc aatttggcga ttgccggaat tgtaaaatac
     3961 ttcccggaca aacctgctta gtgcttcctt acccggattt gagccaccca actgcagatg
     4021 ggtaataacc tcgccttcca aatatttatg aaaaagacct tcctgaatta ctctttgcag
     4081 aggcgtaaca tccgcagaaa cattcaagtg agttgagttt gtataataaa tcgcgccctc
     4141 ggcaatatcg cctttaacat aatgtcccgc cgccggagaa aaatatttta aatccagtct
     4201 ggcgaaacga taagctgttg tttccgccgg cgtttgctcg agcacaaatt tcatccccag
     4261 ctctttactc aggtgtttgg tctcacgctg caagtattca acaactctca gaccaaactc
     4321 aagggcttct ccggattgat gcagctggct gcccagctga atttgtgcca gttcatttaa
     4381 accgataata ccgataatat agtaagcacg gttcattctt aagaagggaa acccatcatt
     4441 gttcatagcc agcaccgcca gcggcccctg ttccgcataa gaaagcagct tttcgataaa
     4501 gtcctttttc tggatatgag cttttactgc cgtagcggca atatctttca acaaactaaa
     4561 tagttttgcc tcatcgccat tggccttgta accaaggcgc ggcaaattca gggttacgct
     4621 ttgaatggcg gccgaacgca tcagccaagg cttatcgagt tccccgctta cggcggcatc
//
LOCUS       DASVIM010000123         3976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1074028, whole genome shotgun sequence.
ACCESSION   DASVIM010000123 DASVIM010000000
VERSION     DASVIM010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074028"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1741)
                     /gene="metG"
                     /locus_tag="VF305_02495"
     CDS             complement(200..1741)
                     /gene="metG"
                     /locus_tag="VF305_02495"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX7416036.1"
                     /translation="MPKSFYITTPIYYVNASPHIGHAYTTIVADVLARYHRMAGEDTF
                     FVTGTDEHGDKIAEAAQKAGVTPQEYADKISAQFRSLWPKLSITNDYFIRTTDTNHIE
                     TVRYILQKVYDAGEIYFGQYEGYYCVGCERFYMEKELVEGKCPDHQTKPEYRKESNYF
                     FRMSKYQDWLINYIKEHPGFIRPERYKNEVLAFLRDPLDDLCISRPKTRLQWGITLPF
                     DENYVTYVWFDALINYVSALDYPQGDKFKKFWPVAQHLIAKDILKPHGIYWPTMLKAA
                     DIEPYQHLNVHGYWNSNQSKMSKSLGNVVRPLDLKDKYGLDAFRYFLLRDMVFGLDSN
                     FSEEAFVKRLNSDLANDLGNLVSRVITMAIKYCDGKVPETSHKKKDSVLHEAALKTVA
                     EVEASFNDMSLHKALIAIWDFINVTNKYIVEKEPWTLGKDPANREKLTAIMYNLLMAL
                     QAIAILISPFMPQAAGKILQQIGVDESKKLALASVYKENVLTAGNPLTRGESLFPRIS
                     RENNS"
     gene            complement(1772..2542)
                     /gene="ricT"
                     /locus_tag="VF305_02500"
     CDS             complement(1772..2542)
                     /gene="ricT"
                     /locus_tag="VF305_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEX7416037.1"
                     /translation="MLTNIIGVKFKKEGRIYSFNATDLILHKDDQVIVNTDNGLSLGV
                     VAVDVKRCQPSELPVNLKKVVRKVTAEDRQRKEENDKLEDEAGKFCLEKIQERGLSMK
                     VINVDCLFDKSKFIFYFTAESRVDFRELVKDLVQRFKTRIELKQIGARQEARITKGIA
                     VCGRMVCCAGILQNLERVTVKMAKEQNMSLNPEKISGLCGRLMCCLAFEHEGYAGQKK
                     CPIATKANGNDAADISDTLLPEDKQTIDLEKMDEENDF"
     gene            complement(2564..3544)
                     /gene="holB"
                     /locus_tag="VF305_02505"
     CDS             complement(2564..3544)
                     /gene="holB"
                     /locus_tag="VF305_02505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX7416038.1"
                     /translation="MSFHDIYGHEKQIGILQKALTQKRIGHVYLFSGIPAVGKKTLAG
                     EFAKALNCEKEDALHDSCGECLSCRKIKQGSHPDVFSVEAEGQFIRINAIREIQEQMK
                     FKPLEARWRVFVIDNADKMNDQAANALLKTLEEPPPANILILVTARPYSMPSTIISRC
                     RHMRFNPLSNAAVAQYLIDRMGLDRHKALLLAGLSGGSLGHAVELNRDDIISYRTGLL
                     KLLSLTQKNDPFSLINFASFLGEGKKEIRQGLNILNTFFRDALVFKETRKIDMLVNQD
                     SSSFISTHAARLSGEQILQNIALVEKAGETIEQNVNKSLTLETMAFKLNY"
     gene            complement(3582..>3976)
                     /gene="tmk"
                     /locus_tag="VF305_02510"
     CDS             complement(3582..>3976)
                     /gene="tmk"
                     /locus_tag="VF305_02510"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7416039.1"
                     /translation="TQDRVVLCDRFSDATYAYQGAGRGLDYEFIKFINEYSAMQLKPD
                     LTLLFDLPVEIGLQRAMERDNRLKDSSFSDRFERENLDFHKRVREGYLNIYKNEPDRC
                     RLIDATRDVDTIQEEVRQYIIDSIGLEN"
BASE COUNT         1099 a          887 c          762 g         1228 t
ORIGIN      
        1 atcggaagcc ttgatgtcat ctttcatttg aaatcgtaat aaaaatcggc ggtctacttt
       61 tccggggaaa atagctaacg aatttttgct cccgcttgtg tttcacggtc gaatatcagc
      121 acggtaaact ttttatcttt atcggtagcc agaagtatac tgcttttcaa tcgattgcag
      181 aggttttctt acctggtgct taactgttat tttctcggct gatgcgcgga aataatgatt
      241 cgccgcgggt caaaggattg cccgcagtta aaacattctc tttatagaca ctagccaaag
      301 ccagcttttt ggattcatcc acgccaattt gctgcaaaat tttccccgcg gcctgcggca
      361 taaaaggaga tatcaaaata gctatcgcct gcaaggccat aagtaaatta tacataatcg
      421 ctgttaattt ttcccggttc gccgggtctt ttcccaaagt ccacggctct ttttccacaa
      481 tgtatttgtt cgtgacattg ataaaatccc aaattgcaat caatgctttg tgcaaggaca
      541 tatcattaaa actagcttcg acttcggcga ctgtttttag ggcagcttcg tgcagcactg
      601 aatctttctt tttatggctc gtttccggca ctttaccatc acagtacttg atggccatgg
      661 tgatgacgcg gctaaccaga ttgcccaaat cattagccag gtctgagttt aatcttttta
      721 caaaagcttc ctcggaaaaa ttggaatcca gtccgaacac catatcgcgc aaaaggaaat
      781 agcgaaaagc gtccaagccg tatttatctt tcaaatcaag tggccggaca acattaccga
      841 ggcttttaga cattttgctt tgatttgaat tccaataacc atgcacattc aagtgctgat
      901 aaggttcaat gtccgccgct ttgagcattg tcggccagta aatgccatgc ggttttaaaa
      961 tatctttggc aatgagatgt tgggccaccg gccagaattt tttaaattta tctccctgag
     1021 gataatccag cgcgctaaca taattaatta aagcgtcaaa ccagacatag gtcacatagt
     1081 tttcatcgaa cggtaaagta atgccccatt gcaggcgtgt cttgggacgg gaaatgcaaa
     1141 gatcgtccaa gggatcgcgt aaaaaggcca acacttcgtt tttgtacctt tccgggcgga
     1201 taaagccggg atgttctttg atgtaattaa tcagccaatc ctgatacttg ctcatccgga
     1261 agaagtaatt gctttcttta cggtattcag gcttagtttg atgatcggga cattttccct
     1321 ccaccaactc cttttccata taaaatcgct cgcaaccaac acaatagtaa ccttcgtatt
     1381 ggccaaaata aatttcacca gcatcataaa ctttttgcaa aatataacgt acagtttcga
     1441 tatgattagt atcggttgtt cgaataaaat aatcgttagt tatagaaagc ttcggccaca
     1501 agctgcgaaa ctgtgcactg attttgtccg catattcttg gggagtcact ccggcttttt
     1561 gcgccgcctc agcgatttta tcaccgtgtt catcagtccc cgtaacaaaa aatgtgtcct
     1621 ctccagccat gcgatgatat ctagccagaa catcggcaac aatagtcgtg taagcatgac
     1681 cgatatgcgg cgaagcatta acgtaataaa ttggtgttgt aatataaaac gatttcggca
     1741 tatttttaaa aagctttccc tttaatcatt attaaaaatc gttttcttca tccatttttt
     1801 ccaaatctat tgtttgtttg tcttccggta aaagcgtatc agatatatcc gctgcatcat
     1861 taccgttagc tttagtagct attggacatt ttttttgccc ggcatatcct tcatgttcga
     1921 aagctagaca gcacatcaag cggccgcaca aacctgaaat cttttccgga ttaagcgaca
     1981 tgttctgctc tttagccatt ttgacggtaa cccgctccaa attttgcaaa attccggcac
     2041 aacaaaccat ccggccgcaa acggctatac ctttagtaat gcgcgcttct tggcgggcgc
     2101 cgatctgttt tagctctatt cgtgtcttga atctttggac gaggtctttg acgagctcac
     2161 ggaagtctac tctactctcc gcagtgaaat aaaaaataaa tttgcttttg tcaaagagac
     2221 aatccacatt gattactttc atggaaagcc ctctttcctg aattttttct aaacaaaact
     2281 tccctgcttc atcttccagc ttatcgtttt cctctttacg ttgcctatct tctgccgtca
     2341 ctttgcggac gacctttttg agatttaccg gtagttctga cggttggcat ctttttacat
     2401 caacagcaac aactcccagc gacagaccat tatccgtatt gacaataacc tgatcatctt
     2461 tatgaagaat aagatcggta gcgttgaaac tatagattcg gccttccttt ttaaacttaa
     2521 cgccaataat gttagtaagc aatcttcatc ctacctgaat tttttaataa tttagcttaa
     2581 atgccattgt ttccaatgtc aatgatttgt tgacattctg ctcgattgtt tcccctgctt
     2641 tttccactag cgcaatattc tgaagaattt gctctccgga taagcgtgcg gcatgagttg
     2701 aaataaaaga agaactgtct tgattgacca acatatcgat ttttcgggtt tccttaaaaa
     2761 ccaaagcatc gcgaaaaaaa gtattaagaa tatttagccc ttgcctaatt tctttttttc
     2821 cctctcccag aaaagatgca aaattaatta agctgaaagg atcattcttt tgtgtaagcg
     2881 aaagcaattt tagaagtccc gtacgatagg aaatgatatc atctctattt aattctaccg
     2941 catgaccaag ggaaccgccg gacaacccag ccaataataa agccttatgc ctatccaagc
     3001 ccattcgatc aataaggtat tgagcgactg ctgcattact tagtgggtta aaacgcatgt
     3061 gccggcaacg tgaaatgatc gtcgatggca tggagtaggg ccgagctgta actaagatta
     3121 atatattggc cggaggcggt tcttccagcg tttttaaaag cgcgtttgct gcttgatcat
     3181 tcattttgtc agcattatca ataacaaaca ctcgccaccg tgcttctagc ggcttaaatt
     3241 tcatttgttc ctgaatttcg cgaatagcat taatgcgaat gaattgaccc tcggcttcga
     3301 cggaaaaaac atcggggtgg ctcccctgct tgattttccg gcaggaaaga cattcaccgc
     3361 aagaatcatg gagagcatct tccttttcac agttgagagc tttggcaaat tcgccggcta
     3421 aagttttttt gcctacggcg gggataccgc taaaaaggta aacgtgccct attcgttttt
     3481 gagttagagc tttttggaga attcctattt gtttttcatg accgtaaata tcgtgaaaag
     3541 acataatcaa tttacaattt aaagggaagc tttccccaaa gttagttttc caagcctata
     3601 gaatcaataa tgtattggcg cacttcttct tgaattgtat caacatcgcg cgttgcatca
     3661 attaagcggc aacggtccgg ttcgttttta taaatattga ggtaaccttc acggactcgc
     3721 ttgtggaaat ccaagttttc tctctcaaat ctatcggaaa aagaggaatc cttcaaacga
     3781 ttatcccttt ccattgctct ctgcagtcca atttcaaccg gcaaatcaaa gagtaaggtt
     3841 aaatcgggct ttaattgcat cgctgaatat tcgttgatga acttgataaa ttcgtagtcg
     3901 aggcctcgcc cggccccttg ataggcataa gttgcatcag aaaaacggtc gcacaagaca
     3961 actctatcct gtgtta
//
LOCUS       DASVIM010000124         4394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_817027, whole genome shotgun sequence.
ACCESSION   DASVIM010000124 DASVIM010000000
VERSION     DASVIM010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817027"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VF305_02515"
     CDS             complement(<1..755)
                     /locus_tag="VF305_02515"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX7416040.1"
                     /translation="MKLGYLLDGVEIIDISGDSSAEVSTICYAADKCTEGSLFVAIAG
                     LKHDGHDFIMDAIARGARFIVHEKDFQFPGQVTAIKVANSRRVLGILAKNYFGNPSAN
                     LTLIAVVGTNGKTTITYLLESIFKAAGFKCGVLGTVNYRYNDNIYPAPNTTPESYELQ
                     KILREMADEGITHVIAEVSSHAIDLRRVDDCDFDLGVFTNLTPEHLDYHLTMENYFQA
                     KKRFFIEVLPQSKKAHPPKMVINGDDRWGQKIL"
     gene            complement(763..2739)
                     /locus_tag="VF305_02520"
     CDS             complement(763..2739)
                     /locus_tag="VF305_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7416041.1"
                     /translation="MAIEAKKWLRFRIVSIFVIFLVLFIALISRAFQLQVLSGQKLKT
                     LAQKQHTSMLQLQPERGVIYDRNGEKLAATIMADSVCADPSKIADPVMASGQIAEILN
                     LDRQTIFKKISEPKNFCWLARRISPDQATSVGNADIEGIFLIKEPKRFYPNGELAAHL
                     LGFVGVDANGLEGLENKYDEVLKGRPEKLAWARDAKGKKLFLRVEKSETKKNEYVDII
                     LTIDNRIQYLVETHLKDAVLSKGAKGGIAIVMDPKTGEILALANEAGFNPNNINGLTP
                     EKWRNRAITDYFDPGSTFKPFLVAGAMEEKIVKESDKFFCENGAFTIADHVIHEAGGK
                     RHGQLAVRDILKYSSNIGSVKIAQKLGKEKFYDYIESFGFGAKTGIDLPGESPGLVRP
                     VQNWARVDTATIAFGQGVSVTAIQLITAVSAIANKGVLMKPYIVRGFADKNNRLIKLY
                     TPTEVRKVISPEVAKRLTNMLTEVVGAADGTGKSARIVNVAVAGKTGTSQKFDFARGV
                     YSSEKVRTSFMGFFPADDPQVAILVILDEPQSDKWGGVAAAPVFKNIGEQILNCFKTN
                     IRETPIFEKDKTNKVQLISTQQALTLQNIASDDESIMPDFIGLTIRDALKKAKAGSIE
                     LKVSGNGWAVHQHPQAGTRLGEERLCNVAFELNN"
     gene            complement(2741..3079)
                     /gene="ftsL"
                     /locus_tag="VF305_02525"
     CDS             complement(2741..3079)
                     /gene="ftsL"
                     /locus_tag="VF305_02525"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX7416042.1"
                     /translation="MVQSAGTQVMPRFREVKKTSFGIKYSTFIVVAIVLMFVALIYVG
                     SHIRMTELEYNIATELNANEHKLEDQKKLKMEYAMLKSPQRIESIARNKLQMSYPDNE
                     QVIVLKSSGE"
     gene            complement(3081..4016)
                     /gene="rsmH"
                     /locus_tag="VF305_02530"
     CDS             complement(3081..4016)
                     /gene="rsmH"
                     /locus_tag="VF305_02530"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX7416043.1"
                     /translation="MNADFYHEPVMVKEVIASLLVNKTGIYVDGTVGGAGHSYAILKE
                     TDAFLVGIDCDDEALQVAEKRLAEFGSRKVLVKANFANLGKVLEGLNIEKVDGVMLDL
                     GVSSHQLDSAQRGFSFNQQALLDMRMDRSLKLSAYDIVNSFAQNELEKIIKFYGEEKM
                     AARIARAISQKRQLSPIKTTAELAAIVASSMPAKLKWQKIHPATRTFQAIRIAVNKEL
                     DNIKPAINDAAEVLKSGGRLCVISFHSLEDRIVKNEFRALAGDCVCPKDMPFCVCQKE
                     AKLKVITRRALTPAAEEIEANPRARSAKLRVAQRV"
     gene            complement(4020..>4394)
                     /locus_tag="VF305_02535"
     CDS             complement(4020..>4394)
                     /locus_tag="VF305_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX7416044.1"
                     /translation="REVFGEKYDNRMVITNWDGYLMVFPYDEWRIIEEKVSHQSILRK
                     EVRAFQRFFMSGAIDCNLDGQGRILIPPALRDYAQLEKDIILAGMIKVIEIWSKERFE
                     DEMKKTGGDIDKFSDFMADLGI"
BASE COUNT         1160 a         1071 c          850 g         1313 t
ORIGIN      
        1 tgcaaaattt tttgccccca tcggtcatcg ccattaataa ccatttttgg cgggtgcgcc
       61 ttcttgcttt gcggcaacac ttcaataaaa aatcgttttt tcgcttgaaa gtaattttcc
      121 attgtcagat gataatcaag atgttcgggt gtaagattgg taaaaacacc caaatcaaag
      181 tcacaatcgt caactcttct taagtcaatc gcgtgagacg aaacttcggc gatgacatgg
      241 gtgatccctt catcggccat ctcccggagt atcttctgca attcataaga ttccggagtg
      301 gtgttcggcg caggatagat attatcattg taacgataat ttactgtgcc taaaacgccg
      361 catttaaaac cggctgcttt gaatatagat tccagaagat aggtaattgt cgttttaccg
      421 tttgtcccga caacggcaat aagcgtcaaa ttggcggaag gattaccaaa atagtttttg
      481 gctaaaatac ccaaaacgcg ccggctgttg gccactttaa ttgccgttac ttgaccggga
      541 aactgaaaat ctttttcgtg aacaataaac cgtgcaccac gggctattgc atccattata
      601 aaatcgtgtc cgtcatgttt taagccggca attgccacaa acagggagcc ctctgtacat
      661 ttgtcagcgg cataacaaat agttgatact tctgccgaag agtctccgga gatatcgatg
      721 atttctactc catcaagcaa ataccccagt ttcatatcat cctcaattat ttagctcgaa
      781 agcaacattg cataagcgtt cctcgcctag tcgtgttccg gcttgcggat gctggtgcac
      841 tgcccaccca ttgcctgata ctttcagctc tattgatccg gcttttgctt ttttcagcgc
      901 atctcttatc gtcaagccta taaagtcagg cataattgat tcatcatcgc tggcaatatt
      961 ttgcagagtc aaagcttgct gtgttgagat cagttgcact ttatttgtct tatctttttc
     1021 aaaaatcggg gtttctcgaa tatttgtttt aaagcaattg agtatttgtt ccccaatatt
     1081 tttaaatacc ggcgctgcgg ccaccccacc ccacttatcg ctttgcggct catcaagaat
     1141 taccaagata gcaacctgtg gatcatcggc cgggaaaaat cccataaaag acgtgcgcac
     1201 tttttccgac gaatatacgc cgcgggcaaa gtcgaatttt tgtgaagtcc ctgttttacc
     1261 ggcaacagcg acattgacaa tgcgcgcact tttaccggta ccatcggcag caccgacaac
     1321 ttccgtcagc atgttagtga ggcgcttcgc cacttcggga gaaataactt tacgcacttc
     1381 tgttggagta tacagcttaa taagacgatt atttttatcg gcaaagcctc gcacgatata
     1441 aggcttcatc aaaactccct tattggcaat ggccgacacg gcggtgatta attgaattgc
     1501 tgtaaccgaa acaccttggc caaaagcaat cgtagcagta tcaactctcg cccagttttg
     1561 caccggcctt actaatcccg gagactcgcc aggcaggtct atacccgtct tcgccccaaa
     1621 accaaagctt tcaatataat catagaactt ttcttttccc agcttttgcg caatttttac
     1681 agaaccgatg ttgctggaat attttaatat atcgcggacg gccagctgcc catgacgctt
     1741 tccacctgct tcatgaataa cgtgatctgc tatggtaaaa gcgccatttt cacagaaaaa
     1801 cttgtctgat tccttaacga tcttttcttc catagcgccg gctaccagaa aaggtttgaa
     1861 ggttgagccc ggatcaaaat aatcggtaat agcgcggttt cgccattttt cgggcgtaag
     1921 cccattgata ttattgggat tgaatcctgc ttcattggcc aacgccaaaa tctctccggt
     1981 tttaggatcc atgacaatgg caattccccc ttttgcgccc ttggagagaa cagcatcctt
     2041 taaatgagtt tccacaagat attgaatccg attatcaatg gttaaaataa tatctacata
     2101 ttcatttttt ttggtctcac tcttttcgac gcgtaaaaaa agttttttac ctttggcatc
     2161 tcgcgcccaa gccagcttct ccggccttcc ctttaagacc tcgtcgtatt tattttccag
     2221 tccttccaac ccgttagcat caacgcctac aaatccgagc aaatgtgcag ccagttcgcc
     2281 attaggataa aagcgtttcg gctctttgat cagaaaaatt ccttcgatgt ccgcatttcc
     2341 cacagaagta gcttgatccg gagaaattct tctggccaac caacaaaagt tttttggctc
     2401 tgatattttt ttaaagattg tttgcctatc caaattgagg atttccgcaa tttgtccgga
     2461 ggccataacc ggatcggcaa ttttggaagg atcggcacaa acagaatcgg ccatgattgt
     2521 cgccgctagc ttttcaccat tacgatcgta aattacgcct ctttccggct gcaactgcaa
     2581 catactggtg tgctgctttt gagctaatgt tttgagtttt tgaccggata aaacctgtaa
     2641 ctgaaaagca cgggatatca aggcaataaa tagcacgagg aaaattacga aaatgctgac
     2701 aatcctgaat cttagccatt ttttcgcttc gatcgccata tcattctccc gaacttttta
     2761 agacaattac ttgctcatta tcaggatagg acatttgtaa tttgtttctc gctatgcttt
     2821 caattctttg cggcgatttg agcatcgcat attccatttt caactttttt tggtcttcca
     2881 atttatgttc attggcattt agttccgtag cgatattata ttccaattct gtcatacgaa
     2941 tatgcgagcc gacatagatc aaagcgacaa acatgagcac tatggcgaca acaataaaag
     3001 tcgaatactt aatgccgaaa gaagtttttt ttacttctcg gaaacggggc ataacttgtg
     3061 ttccagcaga ttgtaccatt ttataccctc tgagctacgc gcaacttagc gctgcgagcg
     3121 cgtggattag cttcgatttc ttctgccgca ggagtcagcg cccttctagt tattactttg
     3181 agctttgctt ctttttgaca gacgcaaaac ggcatatcct tggggcatac acaatctccg
     3241 gccaaagcgc gaaattcatt ctttacaatg cgatcctcca gtgaatggaa agaaatgacg
     3301 caaaggcgac caccggattt tagcacttca gcagcgtcgt taattgccgg ctttatatta
     3361 tccagctctt tattaacggc tattctaatg gcttgaaagg ttcttgtagc ggggtgaatt
     3421 ttttgccatt ttaatttagc gggcatggac gaggcaacta tcgccgctag ctctgcggtt
     3481 gttttaatgg gcgacaactg ccttttttgc gatattgccc tggctattct tgcggccatt
     3541 ttttcttctc cgtaaaactt gataatcttt tccagttcgt tttgcgcgaa gctgttgaca
     3601 atatcgtaag cgctgagttt caagctgcga tccatccgca tgtcgagtag cgcctgttga
     3661 ttaaaactaa agcctctttg agccgaatcc agttgatggg aagaaacgcc taaatccagc
     3721 ataacgccat caactttttc tatattcaaa ccctccagca ctttacctaa attggcaaaa
     3781 ttggctttta ccagaacctt gcgtgaacca aactccgcca atcgcttttc tgccacctgc
     3841 agggcttcgt cgtcacaatc aattcccact aaaaacgcgt ccgtttcttt aagtatcgcg
     3901 taagaatggc ctgcgccccc cacagtgcca tccacataaa ttcccgtttt attaactaaa
     3961 agcgaagcaa taacttcttt gaccattacc ggctcgtgat agaagtcggc attcatggct
     4021 taaattccca gatcagccat gaagtcgctg aacttgtcaa tgtcaccgcc ggtttttttc
     4081 atttcatctt caaaacgttc cttcgaccaa atttcgatta ctttaatcat gcccgctaaa
     4141 attatgtctt tttccagttg cgcataatcg cgcagagccg gcggaattaa aatgcgcccc
     4201 tggccatcca gattacaatc aattgctcca gacatgaaaa aacgttgaaa cgcacgcact
     4261 tctttgcgca aaatagactg gtgtgaaact ttttcttcga tgatgcgcca ctcatcatag
     4321 ggaaaaacca tcaaataacc atcccagtta gtgataacca tccggttgtc atacttctcc
     4381 ccaaagacct cccg
//
LOCUS       DASVIM010000125         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1671090, whole genome shotgun sequence.
ACCESSION   DASVIM010000125 DASVIM010000000
VERSION     DASVIM010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671090"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..659
                     /gene="trxA"
                     /locus_tag="VF305_02540"
     CDS             330..659
                     /gene="trxA"
                     /locus_tag="VF305_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7416045.1"
                     /translation="MSSEFLGIATDNNFEGEVLKSDKPVLIDFWAPWCGPCKAIGPIV
                     EELAGQLKDSIKVMKLNVDENQKTAINYGVRSIPTIILFKDGKVLDTIIGLVPKEKLE
                     DFVKKGL"
     gene            720..1448
                     /locus_tag="VF305_02545"
     CDS             720..1448
                     /locus_tag="VF305_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX7416046.1"
                     /translation="MRLRVVIFALLLLWLFSGCSFFNKREMVKGSPEGLYSSGAVEFQ
                     KSNYKKAREYFLRLKEEYPLHNMAILAEMGIADSYYSDKEYAEAENAYNDFISLHPTN
                     ENVPYAFYQVGMCHYNQIGSIDRDQTEAIKARKELERLVARFPDSKFSTMAGKLIREC
                     KQKMAEQEFYVGQFYFRQNKYQAALARFEKVARDYANVGLDYKIEYFINETKIKIAEE
                     ERLKKVEEEKLKKEVEEKRKTAKK"
BASE COUNT          507 a          288 c          342 g          446 t
ORIGIN      
        1 cgcccttttt taaaagatcg cttcttccca tgattagtgt gctggaaata acgcctaaga
       61 gacccataaa aacctcctcc aattcgcatt cattcgtcta cctttgttgg cttcgtcatc
      121 ggctcctcga cgtataaaaa atacgcctgt gtcgcctctt ccttgccgcc taggtatact
      181 tttgactgca aattggcatt aatttaagtt ttttaatata aaataaatta ccatttagca
      241 atcaaaaact attgccaaat agcgtttgac tttttgtgtt gcgttatgac ataaagatat
      301 gccataaaca gttaaggagg gtgtgactga tgagcagtga atttttaggc atcgccacgg
      361 ataataactt tgaaggtgaa gttctgaaaa gcgacaaacc ggtactcatt gatttctggg
      421 cgccttggtg cggaccatgt aaagctatcg gccctattgt tgaagaactg gccgggcaat
      481 taaaagacag cattaaagtt atgaaattaa atgttgatga aaatcagaaa acggcaatta
      541 attacggcgt gcgcagcata cctaccatta tcctttttaa agacggcaaa gttttagaca
      601 cgatcatcgg ccttgtcccc aaagaaaagc tggaggattt tgtcaagaaa ggcttataaa
      661 tagatagctt accgcgggga agttgtaatt ttatgtattt aaaaatgttc gggaagataa
      721 tgagattaag agtcgttata ttcgctttat tgctgttgtg gctctttagc ggctgttctt
      781 tttttaataa gcgcgaaatg gtgaagggtt ctcctgaagg gctttactcg agcggagctg
      841 tcgaatttca gaaaagcaat tataaaaaag cccgggaata tttcctgcgg ctcaaagaag
      901 aatatccgct gcataatatg gctattttag cggaaatggg gatagctgat tcctattata
      961 gcgataagga atacgcagaa gcggaaaacg cttacaacga ctttatttct ctccatccca
     1021 ctaatgaaaa tgttccttat gccttctatc aggtggggat gtgccattac aaccaaatag
     1081 gatctatcga tcgcgatcag acggaggcga ttaaggccag aaaggaattg gaaaggcttg
     1141 ttgcccgttt tccggatagt aaattttcca ctatggccgg gaagttgatt cgagaatgca
     1201 aacaaaagat ggcagaacaa gaattttatg tagggcagtt ttatttccgt cagaacaagt
     1261 atcaggcagc tcttgcgcgt tttgaaaagg tggcgcgcga ttacgccaac gttggtcttg
     1321 attacaaaat agaatatttc attaatgaaa ccaaaataaa aattgctgag gaagaaagat
     1381 tgaaaaaagt tgaggaggaa aaattaaaga aagaggtgga ggaaaaacgt aaaactgcaa
     1441 aaaaataatg ctaagccgct ttcgttgcgg ctacctttaa atatttttcc agcaaatcga
     1501 taagcagtta gactatctaa acaatcacac accgataatc aaggcaaaaa taaaattcgc
     1561 gcttgcactc atgggtcatt cat
//
LOCUS       DASVIM010000126         4640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1587135, whole genome shotgun sequence.
ACCESSION   DASVIM010000126 DASVIM010000000
VERSION     DASVIM010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587135"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="VF305_02550"
     CDS             <1..1882
                     /locus_tag="VF305_02550"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HEX7416047.1"
                     /translation="EVLLLSTYRKTAKSYIIYRDQHSRLRDITNKMEVDLVDQYLKKA
                     DWKINENSNMDYSLQGLNNYISSEVSKVYWLNEIYSPEIRKAHTDGDFHIHDLSLLSV
                     YCVGWDLFDLLLQGFRGVSGKVESKPANHLRSALGQVVNFFYTLQGEAAGAQAFSNFD
                     TLLAPFIRYDGLTIKEIKQALQEFIFNINVPTRVGFQTPFTNVTLDVTVPKYYADQHV
                     LIGGKPQKETYKEFQEELDLFNKAFLQVMAEGDAKGRVFTFPIPTYSVTREFDWDNPN
                     IQDLWEMTAKYGVPYFSNFINSDMNPEDARSMCCRLRIDNRELEMRGGGLFGSNPLTG
                     SIGVITINMPRIGYLAKSKKDFLQQLEKLMRLAKESLETKRKVLEKFTDGNLYPYTKY
                     YLRNVKERFNEYWKNHFSTIGLVGMNEACLNLLGKNIASANGQAFTKSVLDFMRNKLI
                     EFQKETGNNYNLESTPAEGTSYRLAKIDKERYQDIICASDGKSKDAEPFYTNSTQLPV
                     NYTDDVFEALDLQDEIQTRYTGGTVFHTFAGERVDDPRAVKALVRKICSNYHLPYLTF
                     TPTFSVCPSHGYIKGEQEKCPTCDEACEVYSRVVGYLRPVKQWNKGKQEEFDSRQVFR
                     FQ"
     gene            2095..2784
                     /locus_tag="VF305_02555"
     CDS             2095..2784
                     /locus_tag="VF305_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEX7416048.1"
                     /translation="MKIGGLQKVSLIDYPGVISGTVFLQGCNFRCPYCHNPELVDKKL
                     FGPCLNEKIILDFLNTRKGKLDAVTITGGEPTIQKDLMPFIKRVKKMGFAVKLDSNGS
                     APQVIKELLAEKLLDFIALDIKAPLEKYKRIVQAPVDTQSIKDSIKLILKSKIPHEFR
                     TTVVKSQLGAKDILEIATLISGAQRYALQKFMPTKTLDKKLLKEKAWSDDELEKIKKR
                     LEKEIPSVIVR"
     gene            2856..4448
                     /locus_tag="VF305_02560"
     CDS             2856..4448
                     /locus_tag="VF305_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7416049.1"
                     /translation="MKQKSKKKSDHLTYSDQIKHLTQIGVALSGEKNVGRLLEMILDE
                     ARKLTNANAGTIYIMSDDETELLFAIIQNDSLNVHMGGTGRKIIWSPVKLKNVDGSPN
                     HSNVSAYVAISGEAVNIADVYDAQGFNFEGTKKFDEKSGYRSKSMLVVPLRNHENDII
                     GVLQLLNAQDQVTGDVISFSLESQELTLSLASQAAVVLTNNQLIHELKSLLESFIKII
                     ATAIDEKSHYTGGHIRRVAQLTMTIANKINETKQAPFAAVKFSDDQLQELHMAAWLHD
                     IGKITTPEHIVDKSTKLEKVHDRINDIKTRIEVLKRDYQLVTTTAAKNKSRKSSKIKS
                     IREEIKVLDEDYRFLKKVNNSKEFTKDEVIERIKKIARRKWKVNGKALPLLSKDEIYN
                     LSVRHGTLNEQERNIVNNHASITQKMLSQLPFPKKMRRIASYASAHHEKIDGTGYPIG
                     LAGDKLPLQSRIIALADIFEALIATDRPYKKGKSLAEALKIMESMAKNNHIDADLFEL
                     FLKEKIYADYAKRELALQQIAV"
BASE COUNT         1443 a          923 c         1041 g         1233 t
ORIGIN      
        1 agaagttctc ttactttcca cttatcgcaa aactgctaaa tcttacataa tttatcgtga
       61 tcaacattcc cgcctaaggg atattaccaa taaaatggaa gttgatctgg ttgatcagta
      121 tttaaagaaa gctgattgga agattaatga aaacagtaat atggattatt ctttgcaagg
      181 tttgaataat tacatatctt cagaagtaag caaagtttat tggttaaatg aaatttattc
      241 gccggaaatt cgcaaagcgc atacggatgg cgatttccat atacatgatt taagtttgct
      301 ttccgtttat tgcgtgggct gggatttgtt tgatttgctt ttgcaggggt tccggggggt
      361 atccggcaag gtggaaagca agcctgccaa tcatttgcgc agcgccttag gccaggtggt
      421 taatttcttt tatacgttgc agggagaagc ggccggagca caggcttttt ccaattttga
      481 tacacttctg gcgcctttca tccgttacga tggtttgacc attaaagaga tcaagcaggc
      541 gctgcaggaa tttattttca atattaatgt gcccacgaga gtcggtttcc aaacaccatt
      601 taccaatgta acgttggatg ttaccgttcc caagtattac gccgatcagc atgtgcttat
      661 cggcggtaag ccgcaaaaag aaacttataa ggagtttcag gaggaattag atctcttcaa
      721 taaagcattt ttgcaggtaa tggcggaagg tgatgctaag ggaagagttt ttacatttcc
      781 cattcccaca tacagcgtca ctcgcgaatt tgactgggat aatccaaata tccaggattt
      841 atgggaaatg acggcgaagt atggcgtgcc ttatttttct aatttcatca attccgacat
      901 gaatccggag gatgcccgta gcatgtgctg ccgtctgcgc attgacaatc gggaattaga
      961 aatgagaggc ggcgggcttt tcggctccaa tccgcttacc ggatcaatag gtgtgatcac
     1021 gataaatatg ccgcgcatcg gctatttggc caaatctaaa aaggacttcc tgcaacagtt
     1081 ggaaaaacta atgcggctgg cgaaagagag cttggaaaca aaaaggaaag ttctggaaaa
     1141 gtttaccgat gggaaccttt atccttatac caaatattat ttgcgcaatg tgaaagaaag
     1201 atttaacgaa tactggaaaa accatttttc caccatcggg ctggtcggaa tgaatgaagc
     1261 ttgcttgaat ttattgggca aaaatattgc ttcggcaaat ggtcaggcct ttacaaaaag
     1321 tgttcttgat tttatgagaa acaaactaat cgagtttcaa aaggaaacgg gcaacaacta
     1381 caatctggaa tcaacgccag cagaaggtac atcgtatcgc ttagccaaga ttgataaaga
     1441 aagatatcaa gatattattt gcgccagtga tggtaaatcg aaagatgcgg agccattcta
     1501 cactaattct acacaactgc cggttaatta tactgatgat gtttttgaag cgcttgattt
     1561 gcaggatgaa attcaaacaa gatacaccgg cggcactgtt tttcatacct ttgccggtga
     1621 acgagtggat gatccccggg cggtgaaggc gttggttcgt aaaatctgtt cgaattatca
     1681 tttaccgtat ctcactttta cgcccacttt tagcgtgtgc ccatcacatg ggtatatcaa
     1741 aggtgagcag gaaaagtgtc cgacctgtga cgaggcttgc gaagtctatt cacgcgtagt
     1801 tggctatctg cgtccggtga agcagtggaa caaggggaaa caagaagagt ttgactcgcg
     1861 acaagttttt agatttcagt aacgtataat ctataaaagc tgaatatcat tgtcgggaag
     1921 cccaggcagt ggggagggct ttgatttatt aatatcaaat tgcctttatt cggctacctt
     1981 tgttggcagc gtcgtcggct tcctcaacgt ataaataatg cgcctgcgga gcctcctcct
     2041 tgccgcctcg gtatcctgtt aaatacaaat tgatatcaga gacgaaaagg gaaaatgaag
     2101 atcggtggcc tgcaaaaagt ctcacttatt gattatccgg gtgtcatttc cggtacagtt
     2161 tttttacagg ggtgtaattt ccgttgtccc tattgccaca atcccgaact ggttgataaa
     2221 aaattatttg gcccttgtct aaatgaaaaa ataattctgg attttctaaa cacacgcaaa
     2281 ggaaagctcg acgcagtgac aatcaccggc ggtgagccga ccattcagaa agatttaatg
     2341 cccttcatca agcgcgtcaa aaaaatgggt tttgccgtta agcttgattc caatggctct
     2401 gccccgcaag tgatcaaaga actacttgct gaaaagttgc tcgactttat tgcgctggat
     2461 attaaagcac cgttggaaaa atacaaaagg atcgtgcagg cacctgttga cacacaatca
     2521 ataaaagata gcatcaaact tattctaaaa tcaaaaattc ctcatgaatt tcgcacaaca
     2581 gtggtgaaat cccaactggg ggcaaaagat attctggaaa ttgccacact gatttccggt
     2641 gcccaacgct atgccctaca gaaatttatg cccaccaaaa cactggataa aaagctgctt
     2701 aaagaaaaag cctggtcaga tgatgaatta gaaaaaataa aaaaacgcct tgaaaaagaa
     2761 attccctctg ttatagttag atagttgttg agtaacccga agaggcgatt aacgaaggta
     2821 gcctcaatga agacaaattg actgaaaggt ctgccatgaa acaaaaaagt aaaaagaaga
     2881 gtgaccacct tacctattct gatcaaatca aacatctgac gcaaattggt gtggcgcttt
     2941 ccggtgaaaa aaatgtcggc cgtcttctgg aaatgattct ggatgaagct cgcaaattaa
     3001 caaatgcaaa cgccggaaca atttacataa tgtccgacga tgaaaccgaa cttctttttg
     3061 ccatcattca aaatgattca ctgaatgtgc atatgggcgg gactggcagg aaaataatct
     3121 ggtctcctgt aaagttaaaa aatgttgatg gctctccgaa ccactcaaat gtttcagcat
     3181 acgttgcaat ttccggtgaa gcggtaaata ttgccgatgt ttatgatgct cagggtttta
     3241 atttcgaagg aacgaaaaaa ttcgatgaga aatcaggata ccgttctaaa tcaatgctgg
     3301 ttgttccctt gagaaaccat gaaaacgata tcatcggggt attgcagctt ttaaacgcgc
     3361 aggatcaagt aacgggcgat gtaataagct tttctttgga atcgcaggaa ctgactttat
     3421 ctttggcttc gcaggcggca gtagtcttga ccaataatca actgatacac gaattgaaaa
     3481 gtttattgga atcgtttatc aagattatcg ctacggcaat tgacgaaaaa tcacactata
     3541 ccggcggcca tatccgccgt gtcgcccagt tgactatgac cattgccaat aaaataaatg
     3601 aaacaaagca ggcacctttt gccgctgtaa aattttccga tgatcagcta caggaattgc
     3661 acatggctgc ctggttgcat gatattggca agataacaac tccagagcat attgtggata
     3721 agtcgacaaa actggagaag gttcatgatc gtattaatga tattaagacg cgaatagaag
     3781 ttctcaaaag ggactatcaa ttggtgacga caacagcagc aaaaaataaa agcaggaaat
     3841 cttctaaaat taagagcatt cgggaagaaa taaaagtgct ggatgaggat tatcggtttc
     3901 ttaaaaaagt taataatagc aaggagttta ctaaagacga agtaattgag aggataaaaa
     3961 aaatcgcgag gcggaaatgg aaagtaaatg gaaaagcttt accgctactc tctaaggacg
     4021 aaatttataa tttgagtgtc cgtcacggaa cgctcaatga acaggaaaga aacatagtta
     4081 acaatcatgc ctcgataacg caaaaaatgc tctcccaact cccttttccc aagaagatgc
     4141 gccgaattgc gagttacgcc tccgcgcacc atgaaaaaat tgatggcaca ggctatccga
     4201 taggtcttgc gggggataag cttcccctgc aatcgcggat tattgccctc gccgatattt
     4261 ttgaagcgct tatcgccacg gataggcctt ataagaaagg gaagagtctt gccgaggcgc
     4321 taaaaattat ggagtctatg gctaaaaata atcatattga cgcggatttg tttgagctat
     4381 ttttgaagga aaaaatctac gctgattatg ctaaacgcga gctggcactg cagcaaatcg
     4441 ccgtctgacg acttcgtaaa tcgtccccgg cattcgccgg gctacgcata tgcgaagtat
     4501 atccggcgca tgccggatgc gctgcatccg tagtcgttgc agcgtataac ctaaaggtca
     4561 cacgtaaaaa aatacgcctc actcccccgg cttcgcgcgc cttgcctctg gaacatttta
     4621 cgaagccgtt tcgggtgagt
//
LOCUS       DASVIM010000127         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1502165, whole genome shotgun sequence.
ACCESSION   DASVIM010000127 DASVIM010000000
VERSION     DASVIM010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502165"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VF305_02565"
     CDS             <1..883
                     /locus_tag="VF305_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX7416050.1"
                     /translation="AISRLEYPAVAALEKLSGFFLKKYGKHTTLDAEALMEDELKTLI
                     DAGSREGVVDESQKELIKRIFALGDKPVTDIMIPRVDMFCLPLNMAVEEIISEVVRKR
                     QERVPIYTDDRDDITGILFTKDLLNDARRVKESKGIGKLLGKPYFVPEEKTINGLLHD
                     FQENGTQIAIVVDEYGGVSGMVTLENILEHLFEEAYGNSGLANNGCEEIDENIISVPG
                     KMPMEQLNNILDAQLPKNEFDTIGGFILHLFGKLPERGEEISSSGYLFRIESVGRTRI
                     LKVRIEKQLPTEQIHEK"
     gene            952..2172
                     /locus_tag="VF305_02570"
     CDS             952..2172
                     /locus_tag="VF305_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX7416051.1"
                     /translation="MALFSSDINKMKFLAGRGSSSAGQAVKLLEKPEWFISTTLIGTN
                     LSIIIASTLATGLFISYFGVVRGEQISLIVMLPTLFIMIIVRSVFQHYAETMAIKTAY
                     FIRWSSVVFYPLAYIIAGISRQAVRFSTDQKVKHASYITKEGLKFILGEKTKGSDILK
                     TEREMVSRVFDFSELTADKIMVPISALTALPIGAKIEEAVQVFTSKKYLRIPVYQDTV
                     YNIIGILHYFDLLETLIGNKDNLENVVAATTIVSFLQTDVFYVPETKKASELLIDMQR
                     RHEHMAIVVDEYGGAVGIVTIEDIGEEIVGSIDDEYTTGERLYKKIAPGRYLINGRME
                     MEDLNQILSIKFPDGNYETLGGFLMNRMGKIPQRRESLEYNGITFIIENADQKSVKEV
                     LVIFPAQLDKFAIK"
     gene            2194..>2863
                     /locus_tag="VF305_02575"
     CDS             2194..>2863
                     /locus_tag="VF305_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7416052.1"
                     /translation="MKDKKFCALILAAGKGTRMKSDLAKVLHSLEGKPLLHYSIEAAR
                     RAGAEKIVVIIGHQAQKIREKFYNSGCIFVEQKPQLGTGHAVLQAKDVLAAYQGLTVI
                     LCGDVPLLKPRTITSLVKSHVTAGAAVTVLTTIPAKPQGYGRIVKQEDGSILKIVEER
                     EATDAEKKIGEINTGIYCVNTPFLFSALTKIDNNNNQKEYYLTDIVEIACRDGEIARS
                     HIVLD"
BASE COUNT          887 a          519 c          677 g          780 t
ORIGIN      
        1 cgctatttcc cgtctggaat atcctgctgt tgccgcattg gaaaaattat ccggcttttt
       61 tttaaaaaaa tacggcaaac atacaaccct tgatgccgaa gctttaatgg aagatgaatt
      121 aaagaccctg atagatgccg gaagccgtga aggtgtggtg gatgagtctc aaaaagaact
      181 gatcaaaaga attttcgcat taggcgataa accggtaacc gacattatga tcccccgcgt
      241 ggatatgttt tgtctgcccc tgaacatggc tgtggaggaa attatatcgg aggtcgttag
      301 gaaaaggcag gaaagggtgc ctatctacac ggatgaccgc gacgatatta ccgggatact
      361 tttcaccaag gatttactta atgacgcgcg gagagttaaa gaatcgaagg gcatcgggaa
      421 attgctggga aaaccttatt ttgtgccgga agagaaaact attaacggac ttcttcatga
      481 tttccaggaa aatggaacac aaattgccat tgttgttgat gaatatggag gggtatccgg
      541 catggtaact ctcgaaaata ttctggaaca cttatttgaa gaagcctatg gcaatagcgg
      601 attggcaaat aacggttgcg aagagattga cgaaaatata attagtgttc ccggcaaaat
      661 gccgatggag caattaaata atatactgga tgctcaactg ccgaagaatg aatttgatac
      721 catcggcggt ttcatcttgc acttattcgg aaaactgccg gaaagaggag aggaaatcag
      781 ttccagtggt tatttatttc gcatagaaag tgtaggcagg acaagaatat tgaaagtcag
      841 gatagaaaag caattgccaa cggagcaaat tcacgaaaaa tgaatgtata tttaatcatt
      901 ggcgtaattt ttatttgtct cgtattggaa gctctctagt ccggcggtga gatagctcta
      961 ttttcttcgg atataaataa aatgaaattt ttagccggcc gcggttcttc ttccgccgga
     1021 caggcagtaa agttattaga aaagccggag tggtttattt cgacaacact tatcgggact
     1081 aatctttcca taattatcgc ctccactctg gccacaggac ttttcatttc ttactttgga
     1141 gtagttcgcg gcgaacaaat ttcgcttatt gttatgctgc caactttatt catcatgatc
     1201 attgtccgca gtgtttttca gcattatgcc gaaacaatgg ccattaaaac agcctatttt
     1261 atccgctggt cctcggttgt gttctaccct ttggcgtata tcattgcggg aatttcccgg
     1321 caggctgtac gtttttccac agaccagaaa gtaaagcatg cttcttatat taccaaggaa
     1381 ggtttaaagt tcatattagg cgaaaagact aaaggcagtg atattttaaa aacagaaagg
     1441 gaaatggtca gccgcgtgtt tgatttttcc gaacttaccg cggataagat aatggtcccc
     1501 atttcggcgt tgacggcttt gccgatagga gccaaaattg aagaagcggt tcaagtcttt
     1561 accagtaaaa aatatctgcg tataccggta tatcaggata cggtttataa cattattggt
     1621 attttacatt actttgatct gctggaaact ttgattggta ataaagataa tttggagaat
     1681 gttgttgccg caacaaccat tgtttccttt ttgcagaccg atgtgtttta cgtgccggaa
     1741 acaaaaaaag ccagcgaact tctgatcgat atgcaaagaa gacacgaaca catggctatt
     1801 gttgttgatg aatatggggg cgccgtcggc atcgtaacta tagaagatat cggagaagaa
     1861 atagtcggca gtattgatga tgaatataca actggtgaaa ggttatataa aaaaattgct
     1921 cccggacggt atctgattaa cggccgcatg gaaatggaag atttaaatca gatactatca
     1981 ataaagtttc ctgatggcaa ttatgagaca ttgggaggtt tcttaatgaa tagaatgggc
     2041 aaaattccgc agagaagaga aagcttggag tataacggca ttacatttat tatagaaaat
     2101 gccgatcaga aatccgttaa ggaagtattg gttattttcc cggcgcaatt ggataaattt
     2161 gcgattaaat aatttttgaa atgatggcaa tctgtgaaag ataaaaaatt ttgtgcattg
     2221 attttagcgg cgggtaaggg cacccgcatg aaatcggatt tggccaaagt gctccattct
     2281 ttggaaggaa agcctttgct gcattattcc atagaagctg cccgcagagc cggtgctgag
     2341 aaaattgtgg tcattatcgg tcatcaggca cagaagatac gggagaaatt ttacaatagc
     2401 ggatgcatct ttgtggagca aaaaccgcaa ttaggaacgg ggcacgccgt tttacaagca
     2461 aaggatgtgt tggctgctta tcagggttta actgttattc tctgcggaga tgtgccgctg
     2521 ctaaaaccca ggacaattac atcgctggtg aaaagccatg taaccgcggg tgcagcagtt
     2581 accgtgctta cgacaatacc ggcaaaacct caaggatatg gacgcatagt taagcaggag
     2641 gacgggagta ttctgaaaat agtcgaagag cgcgaggcga cagatgccga aaaaaaaatc
     2701 ggcgaaatca acacgggtat ctactgtgta aatacgccat tcctattttc ggcgctcacg
     2761 aaaatcgaca acaacaataa tcaaaaggaa tactatttga cggacatagt cgaaatagct
     2821 tgccgcgatg gtgaaatagc gagatcacac atagttctcg att
//
LOCUS       DASVIM010000128         5304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1160682, whole genome shotgun sequence.
ACCESSION   DASVIM010000128 DASVIM010000000
VERSION     DASVIM010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160682"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VF305_02580"
     CDS             <1..949
                     /locus_tag="VF305_02580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX7416053.1"
                     /translation="KEMGIVIGGGSGGLLGAESFYSELLQKGVEKARFSKLAAVYCAS
                     SADRIASNFGFAGPKATFMTACSAGATAIGYARDLILNGHASIMLAGGVEPMCRITFA
                     AFNALKSIDPEVCRPFDKKRAGLSLGEAAAIMVLEPLDVAVNRGAKIYGEVLGYGVTC
                     DSFHMTAPDEKASGAVRSMQAALKDSGLSIDDIDYINAHGTATPVNDIMETIAIKEVF
                     GQRAYSIPVSSTKSIHAHTLGAAGALEGIVSVLALKHGFIPPTINYRDCDPQCDLDYV
                     TDGTRKADLRNVLSNSFAFGGNNTTIIFGKYAERGAGNG"
     gene            942..2192
                     /locus_tag="VF305_02585"
     CDS             942..2192
                     /locus_tag="VF305_02585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX7416054.1"
                     /translation="MAKNRRIDGAMRIAVTGIGMVTPLGIGKEEFGCKLFAGDCAIDA
                     IKSFDASSFPSHLGAEVNNFQPRDFISVKNLRRMDKISLMTSAAARLTLDDAGLQVNL
                     ANRDRIGIILGTSFGATDVTVQFAGTLLTEGPALVNPILVPNTVMNAAAGHASIELGF
                     RGINTTITHFAVSAETALAYAVAEILRGTADFVLTGGVDILSKFYYETLTRFHALSPV
                     GEGQERCLPFDEARNGVVAGEGCGIICLEKWESAMERGRKPYCEIKGAGMGSSPTTPT
                     GWPDNPEGIKRTISRALENAGVAAGEIQAIYAAANGGKILDAVEAEAYSGIFNGSKKR
                     PLITSIKGAIGESFSSGGMRACALALSMEKGMLPPTVGLTNPVSPLAFVTGEKIEMSI
                     DHAALAGISFGGTYVYLVFANCDK"
     gene            2204..3727
                     /locus_tag="VF305_02590"
     CDS             2204..3727
                     /locus_tag="VF305_02590"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7416055.1"
                     /translation="MNIGEWISKRAILQPDKPFLMEKDKAYNNRQFNERVNKVAHALG
                     RLGVTKGERVALLMSNCSEFLEIFFACSKTGAIMVPLNFRLAVPEILYILQDSAPSVL
                     IYSSEFASKVQEIKAGGILLKRYMCLGGEKLKDDPVFLEYVNSFPESEPLTEVSVDLN
                     DPLFILYTSGTTGDPKGAVLTHQNTLFGAIHSLLGYGVNRSYKSLVVAPLFHIAALDA
                     AVTPIIYAGGSVMISSFYNASEILKTICQEKINYMFAVPVMFQMMTEAEGWKDADLGH
                     VHFFISGGAPISLPIIKKYQEEKNVSFVQGYGLTETGRLTSLDLEDSISKAGSVGKEV
                     FHVNLRIVDEKGNNLRPNETGEIIVQGPNVFAGYWRKDTAIASAMKDDWFHTGDMGRR
                     DEDGFIYIVGRKMEMIISSGENIYPAEVERALQSLPQIKEAAVVGMPDQKRGEIVGAF
                     IILHKNIQITKNDILTALQGKIAHYKIPQRIFFVDDFPRNQAGKVLKRILKKQLEDN"
     gene            3847..5076
                     /locus_tag="VF305_02595"
     CDS             3847..5076
                     /locus_tag="VF305_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX7416056.1"
                     /translation="MKFKLIYPKWEKLDRQTRFTLPPHGPVVFAATLPDYVDVEFIDE
                     NVQPIDFGEDVDFVGISVMLTAQIKRGWEIADVYRKKGIKVMFGGIATMLHAEETQQH
                     ADAVFLGEAEGRMEKVFEDFKNGKLQKVYNYMQDFPAIETVGTARRSILKRDLYNYRG
                     VQMVDLVHASRGCRFNCYPCCVNFLGGRQFRPRPYDKVIEELASIDNNRLFIVDNSLA
                     QKKEWEIGLFKEMIPLKKKWCCHPIENDDKVLDLAAQAGAWFVYQAIFDSSDFIRDRI
                     KRYHDYGIAVEGSILLGLDYHTEDYIKKLVDFLLEIDLDMAEFTVLTPFPHTRTFDDL
                     HNEKRIISYDWSNYTCGKVVFQPKLLSPEQLQSLYYYAWDKFYEEEPQNYKMFKMLKK
                     VMDKEKLDGTYKQRVRA"
     gene            5110..>5304
                     /locus_tag="VF305_02600"
     CDS             5110..>5304
                     /locus_tag="VF305_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416057.1"
                     /translation="MKKIGKKAAVVIDGGNVSLDDIIAVARGGVSVEISKNSRFVKRM
                     KLTQQMLMESMRKGIAVYGVT"
BASE COUNT         1458 a         1039 c         1361 g         1446 t
ORIGIN      
        1 aaaagagatg gggatagtca taggtggcgg ttccggcggg cttctgggag cagaaagctt
       61 ttatagtgaa ctattgcaga aaggggtgga gaaggcacga ttttctaaac tggcggcagt
      121 ttattgtgca tcctctgccg atcgcatcgc ctccaatttt gggtttgcgg ggccgaaagc
      181 aacttttatg acggcctgct ctgcaggcgc taccgccata ggttatgcgc gggatttgat
      241 cctcaatggc catgcctcca tcatgcttgc cggtggtgtg gaaccgatgt gccggattac
      301 cttcgcagct tttaatgctt taaaatcaat tgatccggaa gtctgccggc cttttgataa
      361 aaaacgcgcc ggcctttctt taggcgaggc agcggccata atggttttgg agcccctgga
      421 tgtagctgtt aatagaggag caaaaattta tggcgaagtt ttgggttatg gtgttacctg
      481 cgattccttc catatgactg cccctgatga aaaggcatcc ggtgctgttc gttccatgca
      541 agcggcgctc aaagacagcg gcctttccat agatgacatt gattatatta atgcgcatgg
      601 tactgctact ccagtcaacg atattatgga aacaatagca attaaggaag tgttcggtca
      661 gcgtgcttat tccattccgg tcagttcgac caaatccatt catgcgcaca cgttaggagc
      721 cgcaggtgct ctggaaggca ttgtttccgt attggcattg aagcatgggt tcatcccgcc
      781 gacaataaat taccgcgatt gcgatcccca gtgcgattta gattacgtga ctgacgggac
      841 aaggaaggct gatttacgca atgttctttc caattccttt gccttcggtg gcaacaacac
      901 tacgataatt tttggcaagt atgccgaaag gggagccggt aatggctaaa aatcggcgaa
      961 tagatggcgc aatgcggatt gctgttaccg gtatcggcat ggtaactcct ttaggcatcg
     1021 gcaaagaaga atttggttgc aaactttttg caggcgactg tgcgattgat gcgattaaat
     1081 cttttgatgc cagttctttt ccttcgcatt taggcgctga agttaataac tttcaaccac
     1141 gggattttat ttcagtgaaa aacctgcgcc ggatggataa aatatccttg atgacaagtg
     1201 ctgctgcgcg tcttaccctt gatgatgccg gcctgcaagt aaatttagcc aatcgcgacc
     1261 gcatcggtat tattttaggg acttctttcg gagcaacgga tgtaaccgtt caatttgcag
     1321 gcactttgct tacggagggt ccggctttgg tcaacccaat tctagtgccg aatacggtaa
     1381 tgaatgcggc agcgggacat gcttcgattg aattgggatt tcgcgggatt aacacaacaa
     1441 taacgcattt tgcggtttca gcagaaactg ctctggctta tgctgtggcc gaaattctgc
     1501 ggggcacggc tgattttgtg ttgactggag gagtggacat tttatctaaa ttttattatg
     1561 aaacgctgac gcggtttcat gccctttctc ccgtgggcga agggcaggag agatgccttc
     1621 catttgatga agctcgtaac ggtgttgtag ccggagaagg ttgtggaata atttgtttgg
     1681 aaaaatggga gtcggccatg gaaagaggca gaaagccata ctgtgaaata aaaggcgctg
     1741 gcatggggtc atcacctacg actcctaccg gctggccgga taaccctgaa ggtataaaac
     1801 gaacaatcag tcgagctctg gaaaatgctg gagttgctgc gggcgagatt caggctattt
     1861 atgcagctgc taacggtggc aagattttag atgccgttga ggccgaagct tactccggga
     1921 tttttaacgg atcgaaaaaa agacctttaa ttacttcgat taagggtgct ataggggaga
     1981 gcttttccag cgggggcatg cgggcatgcg ccttggcctt atccatggaa aaaggtatgt
     2041 tgcccccaac tgtcgggctg acaaatcctg tcagtccgct ggcctttgtt actggagaaa
     2101 aaatagagat gagcatcgat catgcagcgc ttgccggtat ttcttttggc ggtacttacg
     2161 tgtatttagt ttttgctaat tgcgataaat aagggggata aatttgaaca tcggcgagtg
     2221 gatcagcaaa agggcaatac ttcagccgga taaaccgttt ttgatggaaa aagacaaggc
     2281 ttacaacaat aggcagttca acgagcgagt aaataaagta gcgcacgctt taggccgttt
     2341 aggtgtaacc aaaggggagc gcgttgcctt actgatgtct aactgtagcg aatttttaga
     2401 aatatttttt gcgtgcagca aaacgggcgc gattatggtt cctttgaatt tccggctggc
     2461 agtgccggaa attttataca ttcttcaaga ttccgcgcca agcgttttga tttattcttc
     2521 cgagtttgct tccaaggttc aggaaattaa ggctggcggt attctgttga aacgatatat
     2581 gtgcctcgga ggagaaaaac ttaaagacga tcccgtattt ttagagtacg taaatagttt
     2641 tccggaatcg gaacctctaa cggaggtaag tgtcgatttg aacgatcctc tttttatctt
     2701 gtatacttcc ggcacaaccg gtgatccgaa aggtgcggta ttaacccatc aaaataccct
     2761 atttggcgca attcattctc ttttgggcta tggagttaac cggtcttata aatcactggt
     2821 agtcgcgccg ctttttcaca ttgcagccct agacgctgct gtaactccca ttatttatgc
     2881 cgggggcagt gtcatgatta gcagctttta taatgcctca gaaattctca agactatttg
     2941 ccaagaaaag ataaattata tgtttgctgt accggttatg tttcaaatga tgacggaagc
     3001 ggaaggatgg aaggacgccg atctcgggca tgttcatttt tttatttccg gcggcgctcc
     3061 gatttctctg ccgataataa agaaatatca ggaagaaaag aacgttagtt tcgttcaggg
     3121 gtacggactc acggaaacag gacggctcac ttcactagat ctggaagatt caatcagcaa
     3181 agcgggttct gtcggcaaag aagtatttca tgtgaacttg cgcattgttg acgaaaaagg
     3241 aaacaatcta cggccaaacg aaaccggaga aattattgta caggggccga acgtttttgc
     3301 gggttactgg cgtaaagaca ctgcgatcgc atcagctatg aaggatgatt ggtttcatac
     3361 aggcgacatg gggcggcgtg atgaagacgg gttcatttat attgtcggcc gaaagatgga
     3421 aatgattata tcttccggtg aaaatatcta tccggcggaa gtggaaagag ctcttcaatc
     3481 actgccgcag atcaaggaag cggccgtggt gggaatgccc gaccagaaaa gaggtgaaat
     3541 agtcggtgct ttcatcatcc ttcataaaaa tatccaaatt acaaagaatg atattttgac
     3601 cgcgctacaa gggaaaatcg cgcattataa gattccccaa aggatatttt ttgttgatga
     3661 ctttccccgc aatcaagcag ggaaggtctt aaaaaggatt ttaaaaaaac agcttgaaga
     3721 taattaagcg gaagcgtatc gtgcgaagct ctgctgggaa gaaaaggcga gttgggtgga
     3781 ggacaaaaag taattcgctt tattgccaag acaatattgc aataaattaa ttgaaaaaga
     3841 ggaagcatga aatttaagtt gatttatccc aagtgggaaa agctagaccg gcaaacgcgt
     3901 tttacattgc cgccgcatgg accggttgtt tttgccgcta cattgcctga ttacgtggat
     3961 gtggaattta tcgatgaaaa cgtgcagccc attgattttg gtgaggacgt agattttgtc
     4021 ggaatttctg tcatgcttac agctcagatc aaacgcggtt gggaaatcgc cgatgtctac
     4081 cgaaaaaaag gaattaaagt aatgttcgga ggcatcgcca cgatgttgca cgcggaagag
     4141 acacaacagc atgctgatgc cgttttttta ggtgaagcag aaggtcgtat ggaaaaggtg
     4201 tttgaagatt ttaaaaacgg taagttgcag aaggtttata actatatgca ggattttccg
     4261 gctatcgaaa ccgttggaac agcaaggcgc agtatattaa aaagagattt atataattac
     4321 cgcggtgtgc agatggtgga tttggttcat gcatcccgcg gctgccggtt caattgttac
     4381 ccctgctgcg tcaatttctt agggggacgc caatttcgcc cccgccctta cgataaagta
     4441 atcgaagagc tggccagtat cgacaataat aggcttttca ttgttgataa ttcgctggcg
     4501 caaaaaaaag aatgggaaat cgggcttttt aaagaaatga ttccgctgaa aaaaaagtgg
     4561 tgctgccatc cgattgaaaa tgacgataaa gtgctggacc tggcggcgca ggccggcgct
     4621 tggtttgtgt atcaggctat ttttgattcg tccgatttta tccgcgatcg tattaaacgt
     4681 tatcacgatt acggcattgc cgtcgaaggg agcatccttt taggtctcga ttatcacacg
     4741 gaagattaca ttaaaaaact ggttgatttt ttgttggaaa tcgatctcga tatggccgaa
     4801 tttactgtac tcactccttt tccgcatact cgaacgttcg acgatttgca taacgaaaaa
     4861 agaattatca gttacgactg gagcaactat acttgcggga aagttgtttt tcaacccaaa
     4921 ttgctgtccc ctgaacagct acagtctctt tattattatg cctgggataa attttacgag
     4981 gaagaaccac aaaattataa aatgtttaaa atgcttaaaa aagtgatgga taaagaaaaa
     5041 ctagatggta catacaagca gcgcgtgcgc gcttaaagta taatggagat gaaaggagat
     5101 atttggccaa tgaagaaaat tggcaagaag gccgctgtcg ttatcgacgg cggcaatgtt
     5161 agtcttgatg atattattgc cgtagcacga ggcggggtat ctgtggaaat aagcaaaaac
     5221 agccgctttg taaaacggat gaagttgaca cagcaaatgc ttatggaatc gatgcgcaag
     5281 gggattgctg tttatggcgt tacc
//
LOCUS       DASVIM010000129         2962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_561528, whole genome shotgun sequence.
ACCESSION   DASVIM010000129 DASVIM010000000
VERSION     DASVIM010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561528"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..2005
                     /locus_tag="VF305_02605"
     CDS             395..2005
                     /locus_tag="VF305_02605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7416058.1"
                     /translation="MKIKSLGAAREVTGSCFIIETDKARFAVDCGMHQGTDEIEKRNW
                     DVDIYEPARIDFILITHAHIDHSGLLPRIVQKGFRGPIYATEPTGDLVKILLLDSAHI
                     QEVETTWKIKKMQRYGKASNILPLYSTKDAESVGPLIKTEIYNEIFSPGSGIKANFQD
                     AGHILGAAILELFIEENGKSTKLIFSGDIGRRHQLLMKDPITLSEADYLFMESTYGDR
                     NHKGEEDSLNELAEAIQYSYSRGEKVIIPAFAVERTQEMLYSLYLLSRDGRLPKDMPV
                     ILDSPLAIKATEIFRRYRSYLDSETQSLLQNGEDPLDLPQLQFSSTTAESMQINEMKG
                     SAIIISASGMANAGRIKHHLKHNLWRPGASIVFVGFQAEGTPGRRIVDGAKTIRLFGE
                     DISIKAKVWTISGFSAHAGQNHLLDWLGNFLNKKMKIFLVHGEFSAQNQLASLIRERL
                     GFAVTIPEYLEEINLRLGAPIEEFQKPQAAAPEINWESLLDDLGSKISSLSAQQEKLK
                     SLPASKQMEIKNLLQEVDQRMNGIKSDS"
     gene            complement(2018..>2962)
                     /locus_tag="VF305_02610"
     CDS             complement(2018..>2962)
                     /locus_tag="VF305_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX7416059.1"
                     /translation="KKLKQEKRVFIIMRKQLKIINNTLLVFLTFFCVSETWAGTVTDE
                     IGREVNIKRSPQRIVSLAPGITETLYALNLDDKIVGVTTFCNWPPQARQKPRIGGFTN
                     PSIEKIVSLKPDLIIATADGNRKDTVRQLERIGLPVYVTNPSDTDGILKSILHIGEIT
                     NREKDAGKLVEKLQKRLNNITAQIRHKSKPRVFFQIGLEPVITAGKGTLINEVIERAG
                     GINVAGLDTARYPRYSAEGIMAGSPDIILFAPMARDKEFAAGKSYWQKLGGIPAVDNN
                     RIYPIDTDLISRASPRIVDAIEKMALIFHPGIKITKSK"
BASE COUNT          865 a          632 c          654 g          811 t
ORIGIN      
        1 tattctgtta tacattgaca aagtctaaat catcgtatgc ttaaaaaggg aggtttgaac
       61 tatgggtaaa tcaaaagacg tgaagaaaga ttcaaagaaa aaaccaacca aaacagtaaa
      121 agaaaaaaaa gaagccaaaa aattgaagaa ggcagccagg gctagcctgt aaacttgtcg
      181 gaagggtaat tcaaacaact atctcttaat tagaacaaag taaatttttt ctaaaaacgg
      241 aaagccgttt tcgccggtga ctagttttaa gaaatttatt cgttattcct aaatcaataa
      301 ggatagagcg atggggggag tgttttttaa ttccatgggc atttgcctgc aaattggcat
      361 tagttgaggt aaccctcgcc aaaacggagt tttcatgaaa attaaatctc tcggggcagc
      421 aagagaagtt accggctctt gttttattat tgaaacggat aaggcaagat tcgctgttga
      481 ctgcggtatg catcagggta ccgatgaaat agaaaaacgc aactgggatg tcgatatata
      541 cgaaccagcg cgtattgatt ttatcctgat tactcatgcc catattgatc attcgggatt
      601 actcccccgt attgtgcaga aaggatttcg cggccctatt tacgcaaccg agcccacagg
      661 agatctggtg aaaatcttgc tgctcgatag cgcgcatatt caggaagtcg aaacaacatg
      721 gaaaatcaaa aaaatgcagc gttacggtaa agcttccaat attctgccgc tttattcaac
      781 gaaagatgcc gaatccgtcg gcccgttaat taaaaccgaa atctacaatg agatttttag
      841 tcccggaagc gggataaaag ccaactttca ggatgccggc catattttgg gcgcggccat
      901 actggaatta ttcatcgagg aaaacggcaa atccacaaaa ttaattttct ccggcgatat
      961 cggccgtcgc catcagctat tgatgaaaga cccgatcact ctcagcgagg cggattatct
     1021 ttttatggaa tcaacttatg gcgaccgcaa tcacaaaggt gaagaggaca gcttaaatga
     1081 actggccgaa gctattcagt acagttactc cagaggtgaa aaagttatca ttcccgcctt
     1141 tgccgtggaa agaacccagg aaatgcttta ttcgctttat cttctttccc gtgacggaag
     1201 attgcctaaa gacatgcctg taattctgga tagtccgctg gcaatcaaag caacggagat
     1261 attccgccgt taccgttcat atctcgattc cgaaactcag agccttttgc aaaatggcga
     1321 agaccctttg gatctgccgc aactgcagtt ttcttcgaca actgcagagt ccatgcaaat
     1381 taatgaaatg aaaggttcgg caattattat ttccgcgagc ggcatggcca atgccggaag
     1441 gatcaagcat catctgaagc ataatttatg gcgtcctggc gcaagcatcg tttttgtcgg
     1501 tttccaggcg gagggaactc ccggtcgcag gatagtggat ggcgcgaaga caattcgtct
     1561 ctttggtgaa gacattagca taaaggccaa agtatggacc atcagcggtt tttccgccca
     1621 tgccggacag aatcatcttt tggattggct gggtaatttt ctcaataaaa aaatgaagat
     1681 atttttagtc cacggcgaat tttccgcaca aaaccaactg gcctctttaa tcagggaaag
     1741 attgggcttt gctgtgacaa ttcccgaata cctggaagaa ataaatctgc gattgggcgc
     1801 tccaatagaa gaatttcaaa aaccgcaggc ggcagcgccg gaaatcaact gggaatcgct
     1861 tttagatgat ctgggatcaa aaatatccag tctcagcgcc cagcaggaaa agttgaagtc
     1921 attacctgca agcaagcaaa tggaaatcaa aaatttatta caagaggttg atcaaaggat
     1981 gaatggcatt aagtctgatt cctagcgaga cagagcctta ctttgatttc gtaattttta
     2041 tccccggatg aaaaattagc gccatttttt cgattgcatc cacaatgcgc ggcgatgccc
     2101 tgctgatcaa gtcggtatca ataggataaa ttctattatt atctaccgca ggtattcctc
     2161 ctaatttttg ccagtagctt tttcccgccg caaattcttt gtcgcgtgcc atgggtgcaa
     2221 aaagaattat atccggagat cccgccataa tgccctccgc actgtagcgg ggatagcggg
     2281 cggtgtccag tcccgcaaca ttaatgcccc cggctcgctc aattacttca ttgattaacg
     2341 tacctttacc ggccgtaatg acgggctcta aaccaatctg aaaaaaaaca cgcggtttac
     2401 ttttgtggcg tatttgcgcc gtgatattgt ttaatctctt ttgaagtttt tccaccagtt
     2461 ttccggcgtc cttttcccga tttgttattt caccgatatg taaaatactt ttcaggatac
     2521 catcggtatc ggaaggattt gttacataga cgggaaggcc gattcgttcc aattgccgca
     2581 cagtatcttt cctgttgccg tcagcggtgg caatgattaa atcgggtttc agggaaacaa
     2641 tcttttctat ggaaggattg gtaaatccac cgattcgtgg tttctgacgc gcctgcggcg
     2701 gccaattgca gaatgtggtt acgcccacaa ttttatcatc aaggtttagc gcgtaaagag
     2761 tttccgttat tcccggagca agggaaacaa ttcgttgcgg agagcgcttt atatttactt
     2821 cgcggccgat ctcatcggtt actgtaccgg cccaagtttc gcttacgcaa aagaacgtca
     2881 aaaaaacaag taaagtatta ttaataattt ttaattgttt gcgcattatt ataaaaaccc
     2941 gtttttcttg tttcagcttc tt
//
LOCUS       DASVIM010000130         4480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_990838, whole genome shotgun sequence.
ACCESSION   DASVIM010000130 DASVIM010000000
VERSION     DASVIM010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990838"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VF305_02615"
     CDS             <1..440
                     /locus_tag="VF305_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HEX7416060.1"
                     /translation="LYGQPDFVSALMPFDYTNYYCPEMGENLIRRFLAMERLIRPETL
                     PDIKLSTNEIEDKLALDEKRQVNIDPGYLSKPHLILATGKGYAHRPYLRDGIYADLTL
                     IYQGKKFCSLPWTYPDYAEEKQILMLSKIRAKYLLQLKMVETI"
     gene            469..1347
                     /locus_tag="VF305_02620"
     CDS             469..1347
                     /locus_tag="VF305_02620"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX7416061.1"
                     /translation="MIKSMTGYGRAEAVLQGRNIAVEAKSVNHRFLEISLRLPSALFP
                     LEMEFKKKVGERFKRGRIEIFIRLEGEGADVSKVNLNLEIAHNYFDVLNRIKEEFGLQ
                     DTINLKTLTGFRDIFTPPAETQLSPEFLSQIESSMQEALAMLMKMRQEEGLAMYQDME
                     MRLNVIREILVTIKLRAPQVVLEYQKRLTERIKELTAGHALDDIRLAQEVALLAEKSD
                     ITEEIVRLQSHISQFVSLLQSDEAEGKKIDFLLQEMNREINTIGSKSNDFEITRQVIE
                     VKSELSKLREQAQNIE"
     gene            1353..1976
                     /gene="gmk"
                     /locus_tag="VF305_02625"
     CDS             1353..1976
                     /gene="gmk"
                     /locus_tag="VF305_02625"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX7416062.1"
                     /translation="MAAKGLFIVVSAPAGTGKSSICQSLLQSCPEIKFSVSYTSRPPR
                     PNEVNGKDYYFISRKEFQARIKQGEFVEWVENYGHLYGTSGKTMEEFLQNGKDLLLDI
                     EPRGAKKIKQKFKGGVFVFVLPPSRTELLKRLEKRGHETDEAIRARLAQAESELKEIS
                     WYDYIIFNEKLKNAVDQLRSIYIAQKCKKSSLLDKTKQFVKKFNDRI"
     gene            1992..2261
                     /gene="rpoZ"
                     /locus_tag="VF305_02630"
     CDS             1992..2261
                     /gene="rpoZ"
                     /locus_tag="VF305_02630"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX7416063.1"
                     /translation="MARITVEDSLMVAQTRFGLVSLASKRARQLLKGSAPLMESKNRE
                     VVLALREIAAGKIVYAHPEYLKGTKEDFKPIPDNTEFVGDEEYLE"
     gene            2242..3006
                     /gene="pyrF"
                     /locus_tag="VF305_02635"
     CDS             2242..3006
                     /gene="pyrF"
                     /locus_tag="VF305_02635"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX7416064.1"
                     /translation="MKNTSNKNARDKLIFALDAANLEEALSWLELLSGHVGMFKVGKE
                     LFTAVGPKIIESIKQRGQKVFLDLKFHDIPNTVARAAEAAVKMDVDMFNVHASGGGRM
                     IQETVSAAWACADKLGKARPMILAVTVLTSLNSADLAEIGFEKSTNDLVLQLAKVAHA
                     AGASGVVASAQDIAALRTSFGDEFIIVTPGIRSSEEIAKDDQKRTLSAYEAIRASANY
                     IVVGRPIREAKEPLDACRQIVQDIAAGLAAKQFFIE"
     gene            3026..3778
                     /gene="rlmB"
                     /locus_tag="VF305_02640"
     CDS             3026..3778
                     /gene="rlmB"
                     /locus_tag="VF305_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX7416065.1"
                     /translation="MEVIYGINPIKVLLRQQETVLERIVVASGRGGSSIKEIIDLARQ
                     NKISIEFQQRKYLDELAGSPDHQGIIGLRRAFVYADLDVIITKRSKSCNFDLILVLDS
                     IMDPQNLGSIIRTAYCLGANGIVIPEDRAARITAAVIKASAGSAEQLPIARVINLAQT
                     LNYLKDKGFWVFGAEAHDGSNLKEMDFNCPVVLVLGGESKGIRPLVKKKCDFLLTIPM
                     AGSFDSLNVATAAGIIQYEILSQRSQAKAVKK"
     gene            3888..>4480
                     /locus_tag="VF305_02645"
     CDS             3888..>4480
                     /locus_tag="VF305_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7416066.1"
                     /translation="MPVFIWEGTTRKDEVKKGEMEAVDELAARGLLRRQGFKSIEIRK
                     KPKDLAEYLPFLAGRIKEKNVVVFCRIFSTMINAGLPLIQCLDLLAQQEENKAFAKII
                     RTVKEDIEGGTSLTDALKKYPKVFDELFVNLIAAGEAGGILDVILGRLSNYMEKAMKL
                     KSKVKGAMTYPIAVLVISTGVVALLLIKVIPVFQKMF"
BASE COUNT         1396 a          802 c         1078 g         1204 t
ORIGIN      
        1 cactatatgg tcagcccgat ttcgtaagtg cgttaatgcc ctttgattat acgaattact
       61 actgcccgga aatgggcgaa aatcttatcc gtcgcttttt ggcaatggaa agattaatca
      121 ggccagagac attgccggat ataaagcttt cgacaaatga gattgaagat aaattggctt
      181 tggatgagaa gcggcaagtt aatattgatc cgggatattt atctaagccc catttgattt
      241 tagccaccgg caagggttac gctcaccggc cttatttgcg ggatggaatt tatgctgatc
      301 tgacacttat ttaccaaggc aaaaagtttt gttctctgcc gtggacgtat ccagattatg
      361 ctgaagaaaa acaaatttta atgttgagca aaataagggc gaaatattta ttacagctaa
      421 aaatggtgga gactatataa aaagttttgt aatcaaacag gtggataaat gattaaaagt
      481 atgaccggtt atgggcgggc agaggctgtt ttgcagggca gaaatattgc tgtggaggcc
      541 aagtctgtaa atcatcgttt tttggaaata tccctgcggt tgccttcagc gcttttcccg
      601 ctggaaatgg agttcaaaaa gaaggttggt gaaagattca aacgtggtcg catcgaaata
      661 tttatccggc tggaggggga aggcgccgat gtttccaaag ttaatttaaa tttagagatt
      721 gcccataatt attttgatgt tttgaaccgg ataaaagaag aattcggcct gcaagataca
      781 atcaatttaa agacgttgac cgggtttcgc gacattttta ctccaccggc agaaacgcaa
      841 ctgagccctg aatttctgag tcaaatagaa agttctatgc aagaagcgct ggcgatgctg
      901 atgaaaatgc gacaggaaga aggcctcgcc atgtatcagg atatggaaat gaggcttaat
      961 gttattagag aaatactggt aacgatcaag ttgcgcgcgc cgcaggttgt ccttgaatat
     1021 caaaagcgct taactgaaag aatcaaggag ctgacagcgg gccatgcgtt ggacgatatc
     1081 cgcttagccc aggaagttgc tctgctggcg gagaaaagcg atatcaccga agaaatagtg
     1141 cgcctacaga gtcatattag ccagtttgta tcgcttctgc aaagcgatga agctgaaggt
     1201 aaaaaaattg atttcctttt acaggaaatg aaccgcgaaa ttaatacaat tggttcaaaa
     1261 agcaacgatt tcgaaataac tcgtcaggtg attgaagtaa aaagtgaact gagcaaattg
     1321 cgtgagcaag cgcaaaacat cgagtagaat ttatggcggc caaaggatta tttatagttg
     1381 tctcagcgcc agcgggcacg gggaaaagca gtatttgcca atcgctactg caaagttgtc
     1441 ccgaaataaa attttcggtt tcctacactt cccggccgcc gcgccccaat gaagtaaatg
     1501 gtaaagatta ttattttatt tcccgaaagg aatttcaggc gcgtattaag caaggtgagt
     1561 ttgtcgagtg ggtagaaaat tatggccact tgtacgggac ttccgggaaa accatggaag
     1621 agtttctcca aaatggtaaa gacttattgc tggatatcga accgcggggg gccaaaaaga
     1681 taaaacaaaa gtttaagggt ggtgtttttg tttttgtgct tccgccgtcc cgcaccgagc
     1741 ttttaaaacg attggagaaa cgcggtcacg aaaccgatga agctattcgg gccaggcttg
     1801 cccaagcgga aagtgaatta aaagaaattt catggtatga ttatataatt tttaatgaaa
     1861 aattgaaaaa tgcggtcgac caattgcgct ctatctatat tgcccaaaaa tgtaaaaaaa
     1921 gcagtttgct cgataagact aagcaatttg taaaaaaatt caatgatagg atttaggagg
     1981 taattaaata aatggcaaga attacagtag aagattcatt aatggttgcc caaacaaggt
     2041 ttggtctggt atctctggcc tcaaagcgtg cacgccagtt actgaaagga tcggcaccgc
     2101 taatggagag caaaaacaga gaggttgtat tggcactgcg cgaaattgcc gccggcaaga
     2161 tagtttatgc ccatcccgaa tatttaaaag gaacaaaaga agatttcaaa ccaattccgg
     2221 ataataccga gttcgttggc gatgaagaat acctcgaata agaacgcccg tgataaactg
     2281 atctttgctc ttgatgccgc taatttggaa gaagcattgt cgtggttgga acttctgtcc
     2341 gggcatgtcg gcatgtttaa agtgggcaag gaattgttta ccgccgtcgg tcccaaaatt
     2401 attgagagca tcaaacaaag aggccagaag gtttttttag atctcaaatt tcatgatatt
     2461 cctaacactg tcgcccgtgc ggccgaagcc gccgtaaaaa tggacgtgga catgtttaat
     2521 gttcatgcat ctggcggcgg caggatgatt caggaaacag tttctgctgc ctgggcttgt
     2581 gcggataaac ttggtaaagc gaggccgatg attttggctg tcacagtact cactagtttg
     2641 aatagcgctg atttggcgga aattggattt gaaaaaagca ctaatgattt ggttcttcag
     2701 ttggctaaag tggcgcatgc tgcgggcgca tcaggtgtag tagcttcggc acaagatatc
     2761 gctgcactac ggacgagttt cggcgatgaa tttattatcg taaccccagg catacgcagc
     2821 tcagaagaaa tagcaaaaga cgatcaaaaa cgaactctca gcgcctatga ggccataagg
     2881 gcaagcgcca attacattgt tgtgggcagg ccgatacgcg aggccaaaga gccattggac
     2941 gcctgtcgcc aaattgtcca ggacattgct gcaggtctgg cagcaaaaca attttttatt
     3001 gaataataaa tatagaaagt agaatatgga ggtaatttac ggcataaatc ccatcaaagt
     3061 tctccttaga cagcaagaaa cagtactgga aaggattgtt gttgcctccg gccggggcgg
     3121 ttcatctata aaagaaataa tcgatctagc ccggcaaaat aaaatatcga ttgaatttca
     3181 gcaacggaaa tatttggacg aattggcggg aagtcccgac catcaaggga ttattggcct
     3241 gcgtagggcc tttgtttatg cggatttaga tgtaataata acaaaacgca gcaaatcttg
     3301 taattttgat ctaattctag ttctcgatag catcatggat ccacaaaatc tggggtcaat
     3361 aattcgcact gcttattgtc taggcgcaaa cggtatagtg attccggaag acagagctgc
     3421 tcggattacc gctgcagtta ttaaagcttc tgccgggagc gccgagcaat tgcctattgc
     3481 cagagtgatt aatttagcgc agacgcttaa ttatttaaag gataagggat tttgggtttt
     3541 tggagcagaa gctcatgatg gaagtaattt aaaagagatg gattttaatt gccctgtagt
     3601 tttggtatta ggaggagagt caaagggcat caggccatta gtgaaaaaga aatgtgattt
     3661 tttgctaacc attcccatgg cgggaagctt tgattctttg aatgtggcca cggctgcggg
     3721 gattatccaa tatgaaatat tgtctcagcg cagccaagca aaagcagtta aaaaataaat
     3781 aatttacggc ttccattaaa ttaatattta acttgttgaa tttacatgga agatagttta
     3841 taatgttcga acttataagc aacccataaa agaaaggatt tctaaatatg ccggttttta
     3901 tttgggaagg cacaacaaga aaagatgagg taaaaaaagg ggagatggaa gcggttgatg
     3961 aacttgccgc tcgcggcctt ttgcgacggc aaggatttaa gtccatcgaa ataagaaaaa
     4021 agcccaaaga ccttgctgaa tatctgcctt ttttagcagg cagaattaaa gaaaaaaatg
     4081 ttgttgtttt ttgccgaata ttttccacga tgataaatgc aggccttccg cttattcagt
     4141 gtttggacct tttagcccaa caagaagaaa acaaagcttt tgctaaaatt attcggacag
     4201 taaaagaaga tatagagggc ggtaccagtt taaccgatgc cttaaaaaaa tacccgaaag
     4261 tatttgacga attatttgta aacttaattg cagctggtga agccggcggt atcttggatg
     4321 ttattttggg acgcctttcc aattacatgg agaaggcaat gaagttgaag agcaaggtta
     4381 aaggtgcaat gacttatcct atagctgttt tagttatatc cacaggtgtg gttgctttat
     4441 tgctcatcaa ggtcattcct gtctttcaaa aaatgttcga
//
LOCUS       DASVIM010000131         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1502961, whole genome shotgun sequence.
ACCESSION   DASVIM010000131 DASVIM010000000
VERSION     DASVIM010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502961"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VF305_02650"
     CDS             <1..436
                     /locus_tag="VF305_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7416067.1"
                     /translation="MNIFLEYYGQHLLDKTKLHPQAEEVLKNFENITKAILTNKRYNF
                     TLAIAQGLNIAKYFVEIIGADSTPFPKPDRRVIDYLLNKYSVAKENTLIIGDGINDIA
                     VAKNSGILSCAYLNGLGNRQDLLSLNADYYCDDLLEINSLFY"
     gene            611..>1513
                     /gene="tilS"
                     /locus_tag="VF305_02655"
     CDS             611..>1513
                     /gene="tilS"
                     /locus_tag="VF305_02655"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX7416068.1"
                     /translation="MIKKVNQTIEKYNLLEKGERVVVALSGGPDSTALLAVLAPIAQA
                     LDICLIVAHFNHRLRGGESDEDEKFSRDLSEKMGLTFVSGKIAKTGVEKGISPEDYYR
                     QQRYNFLNKVAEDHQAQKIALGHNLQDQAQTVLLNLLRGSGLKGLKGFLPMRDGKFIR
                     PLIEVSRRDIISFLNEAGISSRQDSSNENRRYLRNKIRGELIPYLKEKFNPKIEENLA
                     QTAEILRNEDQFIQQYVAHALKSSFIQRQQNRILLKITYINKLSHAIRWRLLKNILES
                     LNPAKNGVSFIHIKSLENLVKSCES"
BASE COUNT          503 a          266 c          321 g          423 t
ORIGIN      
        1 aatgaatata tttttagaat attatgggca acatctttta gacaagacaa aacttcatcc
       61 gcaagcggaa gaagtcttaa aaaattttga aaatataaca aaagctattc tgacgaacaa
      121 gcgctataat ttcactttgg ccatagcgca agggttaaat atagcaaaat actttgtgga
      181 aataattggc gccgattcca cgccattccc caaaccggat cgccgcgtaa tagactattt
      241 gttaaataaa tactcagtag caaaagaaaa tactctcatc ataggcgatg gtatcaatga
      301 tattgctgta gccaaaaatt ccgggatatt gagctgcgct tatttaaatg gactaggtaa
      361 taggcaggat ttgctaagtc ttaatgccga ttattattgt gatgaccttc tggaaattaa
      421 ttctttattt tattaatgag actcgctgaa aataagcgga gtgaagattg aagcgcaagt
      481 cgaattaatc ccggtatact acattatcgg ggtagcaaag tgtatcggga tagcgtaatg
      541 atatcgttgc ttacagcggt tctaatttta gattcagggc gaacctgaga cacattatca
      601 catatttttt atgataaaaa aagttaatca aaccatagaa aaatataatc tgctggaaaa
      661 aggcgagcgt gtggttgtgg cgctatccgg tgggcctgat tctacggcgc ttttggcagt
      721 tcttgcccca atagcgcagg cattagatat atgcttgatc gttgcccatt tcaatcatag
      781 attgcggggg ggagaatcgg atgaagacga aaaattttcc cgcgatttat cggaaaaaat
      841 gggattgact tttgtctccg ggaaaatagc taaaaccggg gtggagaaag gcatttcgcc
      901 cgaagattat taccgccagc aaagatacaa ctttttaaat aaggtagccg aagatcacca
      961 ggcgcaaaaa attgctttag gacataattt gcaggatcag gcccagactg ttttacttaa
     1021 cctattgcgc gggagcggct tgaaaggact caaaggtttt ttaccgatgc gggatggtaa
     1081 atttatccgc cccctaattg aagtatcgcg ccgggacatt atttcttttt taaacgaagc
     1141 ggggatttca tctcgacaag atagttctaa cgaaaacagg cgatatctgc gcaataaaat
     1201 ccgtggggaa ctgattccgt atttaaaaga aaagtttaat ccgaaaattg aagaaaatct
     1261 ggcgcaaaca gcggaaatac tgcgcaacga agatcaattc atccagcagt atgttgctca
     1321 cgctttaaaa tcatcattca ttcaaaggca gcaaaataga attttgttaa aaataacgta
     1381 cataaacaaa ttatcccatg ctatccggtg gagattgctt aaaaatattt tagaaagttt
     1441 aaatcctgca aagaatgggg tttcctttat ccatataaaa tcgctggaga atttagttaa
     1501 aagttgtgaa tcg
//
LOCUS       DASVIM010000132         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_478747, whole genome shotgun sequence.
ACCESSION   DASVIM010000132 DASVIM010000000
VERSION     DASVIM010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478747"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VF305_02660"
     CDS             complement(<1..322)
                     /locus_tag="VF305_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX7416069.1"
                     /translation="MAEQIKTEENKKFTIPEIIPILSLHNALVFPKMMIPLEIVGNDS
                     SLLVDEAMTKDRLVGLIMAKRELTSEKDHYGLEDFHPIGTCAVILRMAKASDSRTQLL
                     LQGIS"
     gene            complement(369..809)
                     /locus_tag="VF305_02665"
     CDS             complement(369..809)
                     /locus_tag="VF305_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7416070.1"
                     /translation="MNFIKIKFGSNFEEEFQKAVHEVFHLVSPVFKYYESIWRPNIDV
                     YESIDEVIILADLAGLNKEELHIEIDHRKIKIAGVRKAIADLKDARYRLAEIPRGYFE
                     RSIALPEAVDAESAVASYTEGVLMIRINKLAVNKTRRIQIKIEE"
     gene            1084..2901
                     /gene="typA"
                     /locus_tag="VF305_02670"
     CDS             1084..2901
                     /gene="typA"
                     /locus_tag="VF305_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX7416071.1"
                     /translation="MKKDHLRNIAIISHVDHGKTTLLNAMLKQTGVFRVNQAVEDRVM
                     DSLDLEKERGITIMAKNTAVDYNGVKINIVDTPGHADFGGEVERSLNMVDGAILLVDA
                     SEGPLPQTRFVLKKALALHLPIILVINKIDRSDARIDEVINDSYDLFIDLGAEEKQIE
                     FPILYTNAKIGVSHKKIGDDSSDLQPLLQTIIEYIPAPQGNDEDIPQFLVTNLDYDSY
                     IGQIAIGRLQSGKLEMNSNYSLCTKEKIVPGIKFTALYTFHGLTKKAATSVESGDIIA
                     LSGVENVSIGDTISSMTDPRPLPRLQVDEPTVSMMFYVNDSPFAGKEGKYLTSRHLLE
                     RLEKEALRNVAIKIKKLDRTDAFEVCGRGELQMAVLIETMRREGYELMVSRPQVITKE
                     QKGKTLEPVERLFLDIPEEFVGKITEKLSIRKGRMESLVNKGSGRVSMEFLIPSRGLI
                     GFRSQFLTDTKGGGVMNSLLEDYAPWFGPIPQRMTGALVADRSGRITSYASFAMEDRG
                     EMIVEIGTEVYAGMIVGERNRSSDLTVNITKEKKLTNMRASTSDTTLILRPSRILSLD
                     QAIEFIAEDELVEITPKSVRLRKMELDATRRQQKSRKDN"
BASE COUNT          861 a          630 c          652 g          793 t
ORIGIN      
        1 ggctgatgcc ctgtaaaagc agttgcgttc ggctatcgga agccttcgcc attctcaaga
       61 tgaccgcgca agtgcctatc gggtggaaat cttccagacc gtaatggtct ttttcactgg
      121 tcaattctct cttggccata atcaaaccga ccagcctatc cttagtcatg gcttcatcga
      181 caagaagtga agaatcgttc ccaactattt ccaggggaat catcattttg ggaaatacga
      241 gtgcgttatg cagtgaaagt atgggaataa tttccggtat ggtaaatttt ttattttctt
      301 ctgtttttat ttgttcagcc attaaaccct ccggtttatt tatcaaaagt gggagttact
      361 cgtagttatt attcttctat cttaatttga atacggcgcg ttttattgac cgctaattta
      421 tttatcctga tcatgagaac gccttcggtg taggaagcga cagccgattc cgcgtccaca
      481 gcctccggca gagcgatact cctttcaaaa tagccgcgcg gtatttccgc cagacgataa
      541 cgggcgtctt ttaaatcggc aatagccttg cgcactcccg caatttttat ttttctatgg
      601 tcaatttcta tatgcagctc ttccttgttc agtcccgcga gatcagccaa aatgattact
      661 tcgtctattg attcatagac atcaatgtta ggacgccaaa ttgattcgta atatttaaat
      721 actggactaa ccaaatgaaa gacttcatgc accgctttct ggaattcctc ttcaaaattg
      781 ctgccaaact taattttaat gaagttcatt tctctcacct attaaattga tgatttatca
      841 acgattatgg taggaatata gtgctccgcc gccgatttgt aaagactatc tttaaaaata
      901 aagttttcct gtcgaagata tataaaaaag aaagaactgg ctaaataaca atctttttgt
      961 tatgaaagag tcgcttaaat taagaaagag tgttttggcc atcccaagtt aagattggtt
     1021 acatccaagt ctatgtctat caagtaaacc agttaaaaag atgacctgaa aaaattaaaa
     1081 tcgatgaaaa aagatcattt aaggaatatt gccattattt cccatgttga tcatggtaaa
     1141 actacactgc ttaacgccat gttgaaacaa acgggagtat ttcgggttaa tcaagccgtc
     1201 gaagaccgcg tgatggattc gctggatttg gaaaaagagc gcggcatcac catcatggcg
     1261 aaaaataccg cggttgatta caatggcgta aaaataaata ttgtggatac gcccggccat
     1321 gccgatttcg gcggcgaggt ggaacgcagt cttaatatgg ttgacggtgc aattctttta
     1381 gtcgatgcca gcgagggccc tttgccgcaa actcgctttg tattgaaaaa ggctcttgct
     1441 ctgcacctgc ccatcatttt agtcattaat aaaattgacc gctctgatgc gcgcattgac
     1501 gaagtgatca acgattccta cgatcttttt atcgatttag gcgccgagga aaagcaaatc
     1561 gaatttccga tactctatac taatgccaaa atcggagtca gccataaaaa aattggcgat
     1621 gacagctctg acttacagcc gctgctgcaa acaataattg aatacatacc cgcgccgcaa
     1681 ggaaacgatg aagatatccc gcagtttctc gttaccaatc tggattatga ttcttatata
     1741 ggacaaattg ccatcggccg tctgcaaagc ggcaaactgg aaatgaatag caactacagc
     1801 ctttgcacca aagaaaaaat agttccgggt atcaagttca ccgcgcttta cacttttcac
     1861 ggtcttacaa aaaaagcggc aacgagcgtt gaatccgggg atattatcgc tttatccgga
     1921 gtggaaaatg tcagtatcgg tgataccata tcatctatga ccgatccacg cccgctaccc
     1981 cgcctgcagg tggatgagcc gacggtatcc atgatgtttt atgtcaatga tagccctttt
     2041 gccggaaaag aaggaaaata tcttacctcg cgccatttgc tggagaggct ggaaaaagaa
     2101 gccctgagaa atgtcgccat taaaattaaa aagttggaca ggaccgacgc attcgaggtt
     2161 tgtggacgcg gcgaactgca gatggcagtt ttaattgaaa caatgcgccg cgaaggctat
     2221 gaactaatgg tttcacggcc acaggttatt accaaggagc aaaaaggtaa aaccttggaa
     2281 ccggtggaaa ggctttttct ggatataccg gaagaatttg tgggaaaaat tacggaaaaa
     2341 ttatccatca gaaaaggacg aatggagagc cttgtcaata aaggtagcgg gagagtcagt
     2401 atggaatttt tgatcccttc ccgcggatta atcggttttc gcagccagtt tttgaccgat
     2461 accaaaggcg gcggagtcat gaattcgcta ttggaagatt acgctccctg gtttggtccc
     2521 attcctcaac gcatgaccgg agctttagtg gctgaccgtt ccggccgtat tacatcttac
     2581 gcgagctttg cgatggaaga ccgcggggaa atgatcgtgg aaatcggcac ggaagtttac
     2641 gcgggcatga tcgtaggaga gcgcaaccgc agcagcgatc ttactgtaaa cattaccaaa
     2701 gaaaagaagc tgacgaatat gcgggcttcc acatccgata cgacactaat tttgcggccg
     2761 tcgcgaattt tatctctgga tcaggctatc gaatttattg ccgaagatga gctggtggaa
     2821 atcactccta aaagcgtccg cctgagaaaa atggaactcg acgccaccag gcgtcagcag
     2881 aaaagccgca aggacaatta attaagttag gaaacagttg cgtcagttcc ctaaaa
//
LOCUS       DASVIM010000133         7653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1247372, whole genome shotgun sequence.
ACCESSION   DASVIM010000133 DASVIM010000000
VERSION     DASVIM010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7653
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247372"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..472
                     /locus_tag="VF305_02675"
     CDS             272..472
                     /locus_tag="VF305_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416072.1"
                     /translation="MALDEPKKEDEVFEEKGTKYIIDKDIYNQAKPINVDFVSTPRGS
                     GFKLTSSLAPDDGDTCGSSCSC"
     gene            518..1465
                     /locus_tag="VF305_02680"
     CDS             518..1465
                     /locus_tag="VF305_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7416073.1"
                     /translation="MAKDNKIEKLDNAGNLLRQGQYREAINLYEEIHKAYPEEESILL
                     MLAWAHYDSGSAEQAIEYLNILLERELQRKVFTGFAFDELVRIYKQDKNFQKLVEICE
                     RAVKAQPEDAGLLTELGNAYLQLGRAKEASAIYEKLIRIDDDNPAFYCYWGEALFAAG
                     LFPESEKAYLHAGKIDPEQAGNYYFKIAFLFQQAGNHREAKRLLDKCIALSQNNPLYY
                     CSLGDSLIGLGQIKEALAAYETAMQYDNSRVGAYYNRLGHTLIKTNHFSQAVEAFKSA
                     IAHDAAQPYYLSLASAYKAMGLADRADEILREVNKIKQD"
     gene            complement(1462..1977)
                     /locus_tag="VF305_02685"
     CDS             complement(1462..1977)
                     /locus_tag="VF305_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416074.1"
                     /translation="MSEEKSTPAPVESEGIDYSGRPFGYLNLDSETAMLCEHDPVIRD
                     KISKALKSLNYDIVESATFKEALRYLRFHIFNVIVIDEEFDTGVGEVNYVLNYLESLS
                     MPIRRQTFVVLISSTFATMDNMRSYNKSVNLIINKKEIDEAGLIFKKEISENNDFYHV
                     FKENIRKHGKV"
     gene            complement(2023..3207)
                     /locus_tag="VF305_02690"
     CDS             complement(2023..3207)
                     /locus_tag="VF305_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7416075.1"
                     /translation="MRKPEIDYILTKMLESNVTISDLILTVDKPFQVEAAGRLVGVDL
                     EPHFEKLTPFQTEIFALNLINQDHRLIETLIKEGSCDLSYELPGVARFRVNIFSQLGH
                     YSIVLRKLESKVPSCAELALPDAYYRIAEEKNGIVLVTGATGAGKTTSLAAVINEINE
                     KKPVHIITLEDPVEYTHSQKEATINQRELGKDFDTFANGLRAALRQAPKVILVGEMRD
                     RDSVDIALNAAETGHLVLSTLHTIDAGQTINRILGMFPVEEENQIRTRLADSLRWIVC
                     QRLLPKVGGGRVAAFEALGTSLRVKDIILHGESEGKTFFDIIQQGKAFGMITFDNCIV
                     DLYEKDLITGDTAKAYASNRSNVSRGIDAIRSARGEKTTDLDALEVDGSYGKAKSDPW
                     KK"
     gene            complement(3232..4314)
                     /locus_tag="VF305_02695"
     CDS             complement(3232..4314)
                     /locus_tag="VF305_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX7416076.1"
                     /translation="MAKIDAFFQLMHDQNASDLHLVSGQQPAVRIRGEIERIKYDVLT
                     NDALKVLLYEITPEHKIKQFEETGDIDFAYEIPTLARYRANYFQQKYGMGAVFREIPN
                     KISTCVDLGLPAVVAKLATLPRGLVLVTGPTGSGKSTTLAAIIDEANRLRKDHIITIE
                     DPIEFVHKSQGCIINQREVGIHTKSFSAALRGALREDPDIILVGEMRDLETISLAVEA
                     ASTGHLVFATVHTTSAAKTVDRVIEVFPASEQLQIRSTLADGLRAVVSQILFKRIDVK
                     SRCVALEICIATPAVRNLIRESKTYQIPSMMQTGKKYGMQLLDDGIMDLYNRGWIGSD
                     DAYIKANDKARFRPLLKYPPTDFTEA"
     gene            4573..4648
                     /locus_tag="VF305_02700"
     tRNA            4573..4648
                     /locus_tag="VF305_02700"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4606..4608,aa:Ala,seq:cgc)
     gene            complement(4670..5683)
                     /locus_tag="VF305_02705"
     CDS             complement(4670..5683)
                     /locus_tag="VF305_02705"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF002699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEX7416077.1"
                     /translation="MKKKDLLNKTVEHIDIKAINAVEIIKAMQKMSFSARDLATAADI
                     YDRMLKEKNCAIILTLAGSTSAAGCMQVYVDLVKNNMVDAIVASGASIVDMDFFEALG
                     FKHYQGTPFVDDKLLRKLYIDRIYDTFIDEEQLQTCDRTIKKIADKLPAGVYSSREFI
                     REMGKYLLKHAKKKDSLVQAAYEQDVPIFCPAFSDSSAGFGLVLHQYERPESHISIDS
                     VKDFRELTMIKIKAGTSGLLMIGGGVPKNFAQDTVVCAEILGKEVPMHKYAIQITVAD
                     VRDGACSSSTLKEANSWGKVDSSCEQMVYAEATTVLPLMASYAYHKGSWKKRKKWRLA
                     KIF"
     gene            6091..7428
                     /gene="xseA"
                     /locus_tag="VF305_02710"
     CDS             6091..7428
                     /gene="xseA"
                     /locus_tag="VF305_02710"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX7416078.1"
                     /translation="MKEILTVSQLNDNIKFLLEETFGFVWVEGEVGSLRRPQSGHVYF
                     TLKDDKSQIRTVYFRQFGRSKQGIGFELEEGLMVLCRARLSVYPPRGEYQLIVESMEP
                     RGVGSLQKAFEQLKAKLAAEGLFDTSHKKELPFLPDRIGVITSPTGAVIKDILNITKR
                     RYPATDILIAPVRVQGIEADVEIIQALRNLQAYGAVDVIIIARGGGSLEDIAPFNDEA
                     LAREIFHSSIPIVSAVGHETDFTICDFVADLRAPTPSAAAELVVPERIELMTRMNNLK
                     ERLVAGYCRYREDKRDQLTGLQERLKDPRRFLIDFQIHLDDLRERLRAALNHEKQNLY
                     NGLRQLELRLRHQSPTRQINEKKIILKNIQKNIFSNFLYHLTTLKERLQKNSAVLESL
                     SPLSVLHRGYSITRSLASGMIIRQADALSIGENVNIQLARGNFDASVKKISQE"
     gene            7548..>7653
                     /gene="xseB"
                     /locus_tag="VF305_02715"
     CDS             7548..>7653
                     /gene="xseB"
                     /locus_tag="VF305_02715"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX7416079.1"
                     /translation="MAKEKFEDALEKLEDIVRKMEAGDIPLNEALKSFE"
BASE COUNT         2180 a         1580 c         1702 g         2191 t
ORIGIN      
        1 aattaataat aagcgacagg agcttgtaat gtcaatttat ataaaaatca ccatataaaa
       61 ctttttccag aatttaattg acatcttgtt aagtatagct atgttaaata agagatagat
      121 ggaaaaatgt tcatcgacgg aaggaggata tttaatgttt acagttacgg ataaagcgtc
      181 ggaagtaatt aaagattttc ttaaggataa gaatgaaagc tctgctgtga gaattataat
      241 gtctcaaggc tgatccggcc cctctttggg catggctctg gatgagccta agaaggaaga
      301 cgaagttttc gaagaaaaag gcacaaagta tattattgat aaagatattt ataaccaagc
      361 aaagccgatt aatgttgatt ttgtgagtac gccgcgaggg tctggattta aattaacttc
      421 cagccttgct ccggatgatg gcgatacttg cggttcatct tgcagttgct agaacttcgg
      481 ggggagaatt gtctccccct tatttttttg tactatgatg gcgaaagaca ataaaataga
      541 gaaactggat aatgcgggga atctcctccg gcaaggtcaa tatcgcgaag caattaatct
      601 ctatgaagaa attcacaaag catatccgga agaagaatct attttattga tgctggcttg
      661 ggcccattac gatagcggat cagcagagca agctatagaa tacttaaata tactattgga
      721 gcgggagctc cagcgaaaag tttttacagg ttttgctttt gacgaacttg tgcgtattta
      781 taagcaggat aaaaatttcc aaaaattggt ggaaatttgc gagagggcag ttaaggcgca
      841 gccggaagat gccggacttt taactgaatt gggcaatgcc tatcttcaat taggcagagc
      901 taaagaggct agcgctatat atgaaaagct cattagaata gatgacgaca accccgcttt
      961 ttattgctac tggggtgaag ctttgtttgc cgccggttta tttccagaaa gtgaaaaagc
     1021 ctacttgcat gctggcaaaa ttgatccgga gcaggctggt aattattact tcaaaattgc
     1081 ttttctattt cagcaagcgg gaaaccatcg ggaagccaaa agattattgg ataaatgtat
     1141 tgctcttagt caaaataacc cactatatta ttgttcatta ggagatagtc ttattggttt
     1201 gggacaaata aaggaagcct tggcggctta tgagacagcc atgcaatacg ataattccag
     1261 agttggagct tactataatc gtttaggcca tacactcata aagacaaacc atttttcaca
     1321 ggcagttgag gcttttaaat cggccatagc tcacgacgct gcgcagccct actatttaag
     1381 cttggcttct gcttacaagg caatggggct tgctgatcgg gctgatgaaa ttctacgtga
     1441 agtaaacaaa attaaacaag attaaacttt tccatgtttg cgaatatttt ccttaaatac
     1501 atggtaaaaa tcattatttt cggaaatttc ttttttaaaa attaggcctg cttcgtcaat
     1561 ttcttttttg ttgataatta agttgacgct tttattgtag gaacgcatgt tgtccatagt
     1621 tgcaaaagtt gagctgatta aaacaacaaa agtttgccgg cgaataggca tagataaact
     1681 ttctaaatag ttgagaacat aattaacctc cccaacaccg gtgtcgaatt cttcatcaat
     1741 gacaatgaca ttaaaaatgt gaaaacgtaa gtatctaaga gcttccttaa atgtggccga
     1801 ttcaactata tcatagttaa gtgatttcaa tgccttacta atcttatccc tgataacagg
     1861 atcatgttcg caaagcatgg cagtttcact atccagattg agataaccaa atggccgtcc
     1921 cgaataatct attccttctg attcgactgg agccggtgtc gatttttctt cactcatgtt
     1981 aatctcctca tcatctctct caagtcacga aaatgatttt tattattttt tccatggatc
     2041 tgatttagct ttgccatatg agccgtcaac ttccagcgca tccaaatcgg tggttttttc
     2101 accacgggcg cttcttatcg catcgatccc ccggctgaca ttagatctat tcgaggcata
     2161 agctttggcc gtatctccag taatcaaatc tttttcgtac agatcgacaa tgcaattgtc
     2221 aaaggtgatc atgccaaaag cttttccttg ctggataatg tcaaaaaatg ttttgccttc
     2281 tgattcgccg tgcaggatga tgtctttgac acgcaagctg gtgcccagcg cttcgaaggc
     2341 ggcaacacgg ccaccgccaa ctttcggcaa caagcgctga cagacgatcc agcggagaga
     2401 atcggccaga cgcgtgcgta tttgattttc ttcctcaacg ggaaacatgc cgagaatacg
     2461 gttgattgtt tggccggcat caatagtatg caaagtgctc aaaacaagat ggccggtttc
     2521 cgcggcattc aaggcgatat ctacggaatc acggtcgcgc atttcaccga caaggattac
     2581 cttgggcgcc tggcgtagag cggcacgcaa gccattagcg aaagtatcga aatctttacc
     2641 tagctcgcgt tggttaatgg tggcttcctt ttgagaatgc gtgtattcta ccggatcttc
     2701 caatgtaatt atatgcacgg gttttttttc attgatttca ttgataacgg cggcaagcga
     2761 tgtagtttta ccggcacccg ttgctcctgt tactaaaaca attccatttt tttcttcggc
     2821 aatgcggtaa taggcatcgg gtaaggctag ttcagcgcag gaaggaactt ttgattcgag
     2881 ttttcttaag acaatagaat agtgaccaag ttgcgaaaat atatttacac ggaaacgggc
     2941 aactccgggc aactcataag acaaatcgca agacccttct ttaattagtg tttctatcag
     3001 ccgatggtcc tgattaatta agtttagagc aaatatttca gtttgaaacg gtgtaagttt
     3061 ttcgaaatgc ggctctaaat caacaccgac taatcggcct gccgcttcga cctgaaaagg
     3121 tttatccaca gttaagataa gatcggaaat ggttacattg gattccagca tttttgttag
     3181 gatataatct atttccggtt ttctcataag ctatcctcgc ctcgcaataa tttatgcttc
     3241 ggtaaagtct gttggtggat atttcaaaag tggacgaaat ctagctttat cgttcgcctt
     3301 aatataagca tcatcgctgc cgatccaacc gcggttataa agatccataa ttccgtcgtc
     3361 caaaagttgc attccgtatt ttttacccgt ctgcatcatg gaaggaattt gataggtttt
     3421 agattcgcgg atcaaattac gtaccgcggg ggtggcaatg caaatctcca gagcaacaca
     3481 gcgggatttg acgtcgattc ttttgaacaa aatttgcgaa acgaccgcac gcaaaccatc
     3541 agctagcgtg gagcggattt gcaattgttc ggaagcaggg aaaacttcaa ttactctgtc
     3601 gaccgttttt gccgcgctgg ttgtgtgcac cgtggcaaaa accagatggc cggtggaggc
     3661 ggcttccaca gcgagcgaga tggtttccag atcgcgcatt tcgccaacca gaataatatc
     3721 cggatcttca cgcaacgctc cgcgcaatgc cgcactgaaa cttttagtat gaattcccac
     3781 ttcacgttgg ttgatgatac aaccttggct cttatgaaca aattcaatag gatcttcaat
     3841 cgtaataata tggtctttac gtaaacggtt ggcttcatca ataatggcgg ccagtgtcgt
     3901 ggatttgccg ctgcctgtgg gaccagtgac caacacaaga ccacggggca gagtagctaa
     3961 ttttgctact acggcaggta gcccgagatc tacacaagtg gaaattttat tggggatttc
     4021 gcggaaaacg gcgcccatac catatttttg ctgaaagtaa ttggcgcgat agcgcgcgag
     4081 agtggggatt tcataagcaa aatcaatgtc gcccgtttct tcgaactgtt taattttatg
     4141 ttccggtgtg atttcgtaga gaagcacttt caaggcatca ttggtgagga cgtcatattt
     4201 gatccgttca atttcgccgc ggattctaac ggcaggctgt tggccggaaa caaggtgaag
     4261 gtcggaagcg ttctggtcat gcataagctg aaaaaaagcg tcgattttag ccatgtattt
     4321 ttctccttgg tgagatcaac tattttctat cacttttctt tccggaaaag aatcaaataa
     4381 tttatgggtg tgaattttac ggccgcatag agatttttat caaccttatc ttatatcaaa
     4441 ttgtttatga ttataggaaa aactcaatga ttttgtcaag ccccgaatca aattaacaat
     4501 ttcttgacac taagacaaac aataatatat gctcgcgcac gtttttctta ctcgacgaga
     4561 agttgaaaga ttggggatgt agctcagctg ggagagcgcg gcgttcgcaa cgccgaggcc
     4621 aggggttcga tccccctcat ctccaccaag aaaaaaatta cgttcatgtt taaaaaatct
     4681 tggctaatcg ccactttttg cgcttcttcc aacttccttt gtgataagcg taactggcca
     4741 tcagcggcag cacagtagta gcttcagcgt aaaccatttg ctcgcaagat gaatccactt
     4801 taccccagga attggcttct ttgagagtgg aactggagca ggcgccgtca cgaacgtcag
     4861 ccacggttat ttgaatagcg tatttatgca tcggcacttc cttgcccaat atttccgcac
     4921 acacaaccgt atcctgggca aaatttttcg gtacaccgcc gccgatcatc aataagccgc
     4981 tggtgcctgc cttgatttta atcatggtta attcgcgaaa atcttttacg gagtcaatag
     5041 agatatggct ctccggccgt tcatattggt gtagcacaag gccgaagccc gccgagctat
     5101 ccgaaaaagc cggacaaaaa atgggaacat cctgttcgta ggcggcttga actagagaat
     5161 ccttcttctt ggcgtgcttc agtaaatatt ttcccatttc gcgaatgaat tcccgcgaag
     5221 aataaacgcc cgcaggaagc ttatccgcta ttttcttaat cgtgcggtca caagtttgga
     5281 gctgttcctc atcaatgaag gtgtcgtaaa tccgatcaat ataaagttta cgtaataatt
     5341 tatcgtcaac aaagggcgtg ccctgataat gcttgaatcc tagagcttca aaaaaatcca
     5401 tgtcgacaat cgaagcgcca gaggccacaa tagcatcaac catattgttc tttaccaaat
     5461 caacgtaaac ttgcatacag ccggcggcag aggtagaacc ggccagagtt aagataattg
     5521 cgcagttttt ttctttgagc atccggtcgt aaatatcggc agccgtagct aaatcacggg
     5581 cggaaaagga catcttttgc atggctttaa taatttccac ggcgttgatg gctttaatgt
     5641 caatatgttc cactgtttta ttcaaaagat ctttcttctt cattaaatag tagctccctt
     5701 tatattattg catctgatac caattcgctt tctttggcta actttgttgg cagcgtcgtc
     5761 agcttcctca acgtattagt aatacgcctg cggagcctcc tccttgccgc ctcgttatcc
     5821 tttgaaagcg aaagggtata aatcttatta aaacatgggc agggaaaagt caatccattg
     5881 taattaatat aattcccgac agtatgctta agttttatca tttttctctt tgggcaggtg
     5941 aaagctatgg cggagatcca gaaggttttt atattaataa cctatggcga aacattggac
     6001 ccattgatgg caatggagac tcattgttac ttaatttcaa tcttgtttta cctctcaata
     6061 tctggtagtc cacaacgcag gattatgcag atgaaagaaa ttcttacagt ttcgcaactc
     6121 aacgataata ttaaattcct tctggaggaa accttcggtt ttgtttgggt tgaaggcgaa
     6181 gtcgggagct tacgccggcc acagtccggg cacgtttatt ttacgctgaa ggatgacaaa
     6241 agccaaatcc gaacggttta tttccgtcaa tttgggcggt ctaagcaagg gattgggttt
     6301 gagttggaag aaggattaat ggtcttgtgc cgcgcacgac tgagcgttta tccgccgcgc
     6361 ggggaatatc agcttatcgt agaatctatg gaaccgaggg gtgttggatc tctgcagaag
     6421 gcttttgaac aactcaaagc caagcttgcc gccgaaggct tattcgatac gtcccataaa
     6481 aaagagctgc cttttttacc tgatagaatt ggagtaataa cctcgcccac cggcgcggtt
     6541 atcaaagata ttttaaatat aaccaaacgg cgctatccgg cgacggatat attaattgcc
     6601 cccgtgcgag tgcaaggtat tgaggccgat gtcgaaataa ttcaagctct gcgcaatttg
     6661 caggcttacg gtgctgttga tgttatcatc attgctcgtg gcggcggttc cctggaagat
     6721 attgcccctt tcaatgatga ggctttggcg cgtgaaattt tccattcgtc aatacctata
     6781 gtgtccgcgg tcggccatga aactgatttt accatttgtg attttgtggc agacttgcgt
     6841 gcgcccacgc cctctgccgc agcagaattg gttgtgccgg aacggattga attaatgacg
     6901 aggatgaaca accttaaaga gaggctggtt gccggttatt gccggtaccg tgaagataag
     6961 cgggatcaac ttaccggatt acaagaaagg ctcaaagatc cgcgccgctt tttaattgat
     7021 tttcaaattc atctggatga cttgagagaa aggcttagag ccgccttaaa tcatgaaaaa
     7081 caaaatctgt ataatggcct tcgacaactg gagttgcgcc tccggcacca aagcccgaca
     7141 agacaaatca acgaaaaaaa gattatatta aaaaatattc aaaaaaatat attcagtaat
     7201 tttttatatc acttaactac gctcaaagag cggctccaaa agaactcggc tgttttggaa
     7261 tctttgagtc ccctaagcgt tttgcatagg ggttacagca ttacacgcag ccttgccagc
     7321 ggtatgatta tccggcaagc ggatgcccta agtatagggg agaatgttaa tatccaatta
     7381 gcaaggggta acttcgatgc tagcgtaaaa aaaatatctc aggagtaagc cccgttagga
     7441 aagcgggcgc ttggcgattt cagatggcgg catgaaacag ccatctgtgt tacaagattt
     7501 taaccccccc acgggagttg ggggctttct ctaacggggt gaaaattatg gcaaaagaaa
     7561 aatttgaaga tgctttggaa aaacttgaag atatagtaag aaaaatggaa gcaggcgata
     7621 taccgttgaa tgaagccttg aaatcttttg aag
//
LOCUS       DASVIM010000134         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_137989, whole genome shotgun sequence.
ACCESSION   DASVIM010000134 DASVIM010000000
VERSION     DASVIM010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137989"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VF305_02720"
     CDS             <1..737
                     /locus_tag="VF305_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX7416080.1"
                     /translation="FPNHFPDPTVEENLAQLIKLVGSKKADLGIAFDGDADRLGVISD
                     KGEIIWGDKLLLLFSRYILQEKPNSTIIGEVKCSQVLFDDIKKHSGRAIMWKAGHSLI
                     KAKMKEEKAVLGGEMSGHFFFADRYFGYDDAIYAAVRLLEILSQTGQKISSLLSDVPQ
                     TYSTPEIRIDCADDKKAKVVENIKRHFQNNPGLIDIDGARIPFEDGWALVRSSNTQPV
                     IVLRFEASSAKSLQKIRNEVENLLII"
     gene            964..1821
                     /locus_tag="VF305_02725"
     CDS             964..1821
                     /locus_tag="VF305_02725"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEX7416081.1"
                     /translation="MNIARRLLLVLLISVFLFGCSSTKVTTEGKLLDGKGQPIAGITV
                     IFKPVKPTVGPEQLEAKTGADGIFRLSGLISSSEYIITPVSDKWKTNVTMKITTGREG
                     QTMVLTNPIVIRFNLLVNGTIVDTKTGLQWLIYTASDLKAGDVLNTAKNIREGGFSDW
                     RLPSKADILGLQEKSASTTAQSEAESARQICCTWVAEPNSEKVEWEFYVDDGNELWSS
                     SKMPSNDRIIIVRNYSGAPATAQAPTAVVPFPVVVPQPQAAAPPAAVTAPAAAEKKDT
                     TGTAQTGKP"
BASE COUNT          595 a          377 c          414 g          543 t
ORIGIN      
        1 atttccccaa tcatttccct gatcccactg tagaagaaaa tttagcccaa ctgattaaat
       61 tagtgggtag caaaaaggcg gatttaggaa tagcctttga cggcgacgct gatcgtctgg
      121 gcgttatcag cgataagggc gaaataatct ggggcgataa actgctcctt cttttttccc
      181 gctatatcct ccaggaaaaa ccaaattcta caatcattgg cgaagttaaa tgttctcagg
      241 ttttattcga tgatattaaa aaacattccg gccgggcgat aatgtggaaa gcaggacatt
      301 cgctgattaa ggccaagatg aaagaagaga aggccgtatt aggcggagaa atgagcggac
      361 atttcttttt tgccgatcgc tatttcggct atgatgatgc tatttacgcg gcagttaggt
      421 tattggaaat tctctcccaa acaggccaga aaattagttc actgctttcc gatgtgccgc
      481 aaacttactc cacgccggaa atcagaattg attgcgctga cgacaaaaaa gccaaggtcg
      541 tggaaaatat aaaaagacat ttccagaata atcctggtct gattgatatt gatggagcgc
      601 gcattccttt tgaggatgga tgggcactgg tgcgttcttc caatacccag ccggtaattg
      661 ttttgcgttt tgaagcgtct tcggcaaaaa gtttgcaaaa aatccgcaac gaggtggaaa
      721 atctgctcat tatttgatca atttatcata aagaattgat aattttgccc agtcgaataa
      781 acgcaccggc aattttgtct ataatttgtt cccctaaaga agtatttaac tgacatttac
      841 ttttattatt taattatttt tttattgaat attttttatt attatgtaat ctttactcag
      901 aaatggtttg aataaacaac ctaaatattg tcaaaagcaa ctgtaggaca aggagggtta
      961 gttatgaata ttgctcgtcg tttgttactt gttttattga ttagtgtttt tttattcggt
     1021 tgcagtagca ctaaagtcac cactgaagga aagttacttg atggaaaagg acaaccaatc
     1081 gctggaatta ctgttatttt taaaccagtt aaacctacag taggtcctga gcaattagaa
     1141 gcaaaaacgg gagcagatgg aatttttcgt ttatccggac ttatctcttc ttccgaatat
     1201 attattacac cggtatccga taagtggaaa acaaacgtaa caatgaaaat tactaccggc
     1261 agagaagggc aaaccatggt tttaactaac cctatcgtca tccgttttaa cttattggta
     1321 aacggaacta ttgttgatac aaaaactggt ttgcaatggc ttatttacac tgccagtgat
     1381 cttaaggccg gtgatgtatt aaatacagca aaaaatatca gggaaggtgg cttcagcgac
     1441 tggagactcc cctcaaaagc agatatttta ggtctgcaag aaaaatcagc ttctacaact
     1501 gcacagtcgg aagcagaatc tgcccgtcag atatgttgta cttgggtagc tgagcccaat
     1561 tcggaaaagg ttgaatggga attctatgtt gatgacggca atgagctttg gtcatccagt
     1621 aaaatgccgt caaacgacag gatcattatc gtcagaaatt acagcggagc ccccgcaaca
     1681 gcccaggctc cgactgcggt agtaccattt cctgttgttg tccctcaacc gcaagcggca
     1741 gctcctcctg cagcggttac tgcaccggct gcagcagaaa aaaaggacac aactggcaca
     1801 gcacaaaccg gcaaaccgta attgttcgct ttgatgcaaa gaaggcaaca ataaataccg
     1861 aagatttagc cgcattaaaa gccttcgata caaaagtgga attaaagggt ttgtgggggg
     1921 atacaaacc
//
LOCUS       DASVIM010000135         6647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1248167, whole genome shotgun sequence.
ACCESSION   DASVIM010000135 DASVIM010000000
VERSION     DASVIM010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248167"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VF305_02730"
     CDS             complement(<1..308)
                     /locus_tag="VF305_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX7416082.1"
                     /translation="MEKDAASKRISDLRKIIEYHNKRYYQQDDPEISDMEYDSLMREL
                     QELELQYPDGDIASSPTQRVGAAPLAKFSSIKHPSAMLSLANAFDPQEIIDFDKRIKR
                     "
     gene            423..914
                     /locus_tag="VF305_02735"
     CDS             423..914
                     /locus_tag="VF305_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX7416083.1"
                     /translation="MEEKKVKVGVISDTHLTGYDDKLRRCVTEYFSDVDMILHAGDLV
                     DLRVLEIFGGKEVKAVCGNMDNPSVKEKLPEHLLFEVKGFKFVLVHGWGSPRGIEEKI
                     LARFDDIDCIVYGHTHKPANYKKGKVLFFNPGSAVDRHFASSRTIGILEIDKDVAGRI
                     INV"
     gene            933..1418
                     /locus_tag="VF305_02740"
     CDS             933..1418
                     /locus_tag="VF305_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX7416084.1"
                     /translation="METIFAPWRMEYIKGEKTEGCVFCKCSVRCNDYVLYEGRYTFVM
                     MNKYPYVSGHLMIIPHRHTGEYSDLSQEERKEIFNLLETSFKALREAMKPAGFNVGMN
                     IGKAAGAGIEEHIHLHVIPRWEGDTNFMSVVGNVRVIPEDMATTAANLAPLFKKYHQE
                     V"
     gene            1423..1728
                     /locus_tag="VF305_02745"
     CDS             1423..1728
                     /locus_tag="VF305_02745"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX7416085.1"
                     /translation="MRIIYLILTIVFAFFIVTFSQFNSELVTIKYYYINDIDVPVYML
                     IFAALLGGVIITGFWGVVERFRLNRTISRLNKTIRDLRKELRANEAPPIIDGQKMHE"
     gene            1706..2221
                     /locus_tag="VF305_02750"
     CDS             1706..2221
                     /locus_tag="VF305_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEX7416086.1"
                     /translation="MDKKCTNNISSLIFDDQKFITDASLGKLAKWLRLLGYDTLVFPK
                     EAGREMLRLAAAEGRIVLTKRQDMLERQFCGRLFLVAGVDLGNQLNDVISKFSLKINQ
                     QKMFTICLKCNERLYPVTQEEVRNLVPPFVFENCTQYNKCPRCQNVYWAGTHQRNAWQ
                     FMEQHIPCYLP"
     gene            complement(2187..2678)
                     /locus_tag="VF305_02755"
     CDS             complement(2187..2678)
                     /locus_tag="VF305_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX7416087.1"
                     /translation="MICAGIITVSDRSSQGIRKDMSGPALAAVLADADIEVCKSLVIP
                     DEKDQIKEAIKKYADIENLDLILTTGGTGVSPRDITPDATLEVIDKQIPGMAEAMRHQ
                     SMNITPYAMISRAIAGIRGRCLIVNLPGSPKGAKENLAIILPALKHAIEKIKGDNTEC
                     AAP"
     gene            complement(2683..4764)
                     /gene="fusA"
                     /locus_tag="VF305_02760"
     CDS             complement(2683..4764)
                     /gene="fusA"
                     /locus_tag="VF305_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX7416088.1"
                     /translation="MAKYESKSLRNLTVVGHGGTGKTSLCESFLFISGKNERLGRVDD
                     STSSMDYEPEEQKRRVSISSAANFIEWEKHKINIIDTPGDSNFAFDIKCSMSVVENAI
                     IVVDAVGGVEFQTQKVWELADEFKLPRIIFINRMDRVRADFNKALESIKSKFAKKVTP
                     VCVPIGAEDKFQGIIDLIGMKAYLFSDNKGIGKAVDIPAETMDEVKSLRGTMVEDIAE
                     ADDELMNKYLDAGELTPEELKAGLKKGVSSGSLIPVVCGSSIKGIGVSLLLDLVVNSF
                     SSPVDRGPAKGKKPGTDNIEERQPVEDAPFSAIVFKTIADPYAGRLTLFRVYSGKLNP
                     DSSIFNSTKKITEKFGHIFFLEGKNQKQAEILIAGDIAGVAKLKETVTGDTLCHEKSP
                     IIFEKVAVPPPIMSFAIEPKTRGDEDKIASSIHRLTEEDPTIVFSRNTQTKEMILSGM
                     GQVHIEVNIEKMKRKFGVEVNLNTPKVPYKETIKGKTNVQGKYKKQSGGRGQFGDCWI
                     DIEPMPRGGGFVFADKIVGGVIPNNYRPAVEKGIVEAAAKGVLAGYPVVDFKISLTDG
                     SYHTVDSSEMAFKIAGSMAFQKGVMDCQPILLEPIVNIDIEIPEEYLGDVIGDLNSRR
                     GRVLGMDTNGSNQIIKGQVPLAEILKYAPDLRSMTSGRGNFTYTQSYYEEVPAYIAEK
                     IIAESKKEKEE"
     gene            complement(4932..6626)
                     /locus_tag="VF305_02765"
     CDS             complement(4932..6626)
                     /locus_tag="VF305_02765"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416089.1"
                     /translation="MNTIFSLLKPRVLSTINNLRMDNERNSWMRIFMFGALGIVFWAG
                     TFIILYRVLFYFQSVQDFGDILAMKLLSMIIMTFFVLLLFSNMINCLSHLYLSQDLPL
                     LHSLPVSAKDIFLSRWLISTFDSSWMVVAFSLPAFLSYGLIYKASIIFYMIVLLAIIF
                     MCLIASALSGIIVLFGATILPAGRIRTILIILSVFLVLILVLVMRLMRPEQLVNPDSF
                     ASAVLYLNSLQTPNSPFLPTTWITDAIKTALNGKIMGSLFNIALTGSCAFMLIFISNI
                     IAHAAYFMGFSKSQTTPQRLFAPVKYKGLSWESLLNFLPREAKAFAIKEIRTFFRDST
                     QWPQVFMMAALIVIYLYNFSVLPLDKSPIKTIYLQNLLSFLNIGLAAFVLSAIAARFV
                     FPAVSMEGEAFWIVQAAPVSIKTFLWIKFFLYYIPLIILAEILIVVSNFLLQVSPFMM
                     GLSTITMFCLVPAVVAMAIGLGAVYPNFKSENPAQVVTSFGGLLFMILCFGLIALVII
                     LEAGPVYFAFMADVHGKQLSLLQITWLIVSFGLALFLCLAAIFYPLRLGEIKLRIR"
BASE COUNT         1926 a         1342 c         1443 g         1936 t
ORIGIN      
        1 atacgcttta tgcgcttgtc aaaatcaata atttcctgcg ggtcaaaagc gttggcgagg
       61 ctcagcattg cagagggatg ttttatggaa gaaaatttag caagcggcgc cgcgcccacg
      121 cgctgcgtag gggatgaagc aatatctcca tcaggatatt gtaattccag ctcctgtagt
      181 tccctcatta agctgtcgta ttccatatcg gatatttcgg ggtcgtcctg ctggtagtag
      241 cgcttattgt gatattcaat gatttttctt aaatcgctga ttctttttga ggcggcatct
      301 ttttccatat taaaatatta actttactta atactaaagt caaattaaaa gcttttcctc
      361 taaaaccagt aaataaaact tgtttttttt gtgtcttacc gctaataatt cagcagttga
      421 ttatggaaga gaagaaggtc aaagttggcg tcatttccga cactcatctg accggttatg
      481 atgataaatt gagaaggtgc gtcactgagt atttcagtga tgtagatatg attcttcatg
      541 ctggagattt ggttgatctg cgtgttttgg aaattttcgg ggggaaagaa gtcaaagcgg
      601 tatgcggcaa catggataac ccttcagtta aagaaaaatt gcctgagcat ttgctttttg
      661 aagtaaaagg atttaaattt gtcctggttc atggctgggg atctccccgg ggtattgaag
      721 aaaaaatatt agctagattt gatgatatag attgtattgt ttatggacat acgcataagc
      781 cggcaaatta taaaaagggt aaagtgctgt tctttaatcc cggctcggcg gtagatagac
      841 attttgcgtc ttccaggact atcggcatat tagaaattga caaagatgta gcaggtagga
      901 taattaacgt ctagttggtg gttttttaaa atatggaaac tatttttgct ccctggcgca
      961 tggaatatat taaaggcgaa aagactgaag ggtgcgtttt ctgcaaatgt tccgtcaggt
     1021 gcaatgatta tgtcctttat gaaggtagat acacttttgt gatgatgaac aaatatcctt
     1081 acgtaagcgg tcatttgatg attattcctc atcggcatac aggtgaatat tcggatctgt
     1141 ctcaggaaga aagaaaagaa attttcaacc ttttagaaac ttcatttaag gcgcttagag
     1201 aagcgatgaa accagccgga ttcaacgtcg gcatgaatat aggcaaggca gccggagccg
     1261 gtattgagga acatatccat cttcatgtaa ttccacggtg ggaaggggat actaatttta
     1321 tgagtgtggt cggaaatgtg cgtgtaatac cggaggatat ggctacgaca gcggctaatc
     1381 ttgctcctct ctttaaaaaa tatcatcagg aggtatgatg cgatgagaat aatttattta
     1441 attctgacaa tagtatttgc tttttttatt gttacctttt cccaatttaa ttcggagctt
     1501 gtaaccatta aatactatta tatcaatgat attgatgtgc cggtttatat gctcattttt
     1561 gccgctcttc ttgggggagt tataattaca ggtttttggg gcgtagtgga gcgatttcgt
     1621 ctcaatcgca cgattagccg tctgaacaaa actattcgtg atttacgtaa agaactacgc
     1681 gccaatgaag caccaccaat aattgatgga caaaaaatgc acgaataata tttcctcatt
     1741 gatttttgac gatcaaaaat ttatcaccga tgcctcttta ggaaaactgg ctaagtggtt
     1801 gcggctttta ggttacgata cgcttgtgtt tccgaaagaa gccggccggg aaatgctacg
     1861 cttagccgct gctgaaggaa gaattgttct taccaaaagg caagatatgc tggagcgcca
     1921 attttgcggg cgtctatttt tggttgcggg cgttgatctt ggcaaccaac tgaatgatgt
     1981 tatcagtaaa ttttctttaa aaataaatca gcaaaaaatg tttacaatct gccttaagtg
     2041 caatgaaagg ctttatcctg ttacgcaaga ggaagtccgc aatttagtgc ccccgtttgt
     2101 tttcgaaaat tgtactcaat acaataaatg tccccggtgc cagaatgttt attgggcggg
     2161 gactcatcaa cgcaacgcct ggcaatttat ggagcagcac attccgtgtt atctccctta
     2221 attttttcaa tggcgtgttt tagcgccggc agaattatag caagattttc ttttgccccc
     2281 tttgggcttc cgggaagatt aactatcagg cagcgtccgc gaattcccgc tatagcgcgg
     2341 gaaatcatgg catagggcgt gatattcatg ctttgatgcc gcatggcttc ggccatgccg
     2401 gggatttgtt tatcgattac ttccaatgtc gcatcagggg ttatatcgcg gggactgaca
     2461 ccggtgccgc cggtagtcaa aataagatca agattttcta tatccgcgta tttttttatt
     2521 gcttccttta tttgatcttt ttcatccggg atgacgagag atttacagac ttcaatatcc
     2581 gcgtccgcaa ggacggcggc aagggcaggg ccgctcatgt ctttacgaat accctgagaa
     2641 ctgcggtcgc ttaccgttat aattccggcg caaatcatag ttttattctt ctttttcttt
     2701 tttcgactcg gcgataattt tttccgcaat atatgcggga acttcttcat aataggattg
     2761 agtgtaggta aaatttcccc ggccactggt catggaacgc aaatcaggag cgtattttaa
     2821 gatttccgcg agcggtacct ggcctttaat tatttgattg gaaccattgg tgtccatacc
     2881 cagaacccgg ccgcgccggc tgtttaaatc gccaatgaca tcgcccaagt attcttcggg
     2941 tatttctata tcgatattca ctatcggctc cagcagtata ggctgacagt ccatcacgcc
     3001 cttttgaaag gccatggaac cggcgatttt aaaagccatt tccgatgagt ccactgtgtg
     3061 gtaggagccg tccgtcagtg atattttgaa atcaaccacc ggataaccgg ccagaacgcc
     3121 tttcgctgcc gcttccacaa ttcctttttc tactgctggc ctatagttat taggaataac
     3181 gccgccgaca attttatcgg caaacacaaa accgccgcca cgtggcattg gctcgatatc
     3241 aatccagcaa tcgccgaact gtccacgtcc accggattgc ttcttgtatt tgccctggac
     3301 gtttgttttt cctttgatgg tttctttgta gggaacttta ggcgtattca ggttgacttc
     3361 cactccaaat tttcttttca tcttttcaat atttacttct atgtgcacct gtcccatgcc
     3421 ggaaagaatc atttcttttg tttgtgtgtt acgggaaaat actattgtcg gatcctcttc
     3481 cgtgagccga tggatggacg aggcgatctt gtcttcgtca cctcttgttt ttggctcaat
     3541 ggcaaaagac ataatgggtg gcggaacagc taccttttca aagataatcg gagatttttc
     3601 atggcataaa gtgtcgccgg ttaccgtttc ctttagtttg gccacaccgg caatatcacc
     3661 ggcgataagg atttccgctt gtttttgatt tttaccttcc aagaaaaaga tgtgtccgaa
     3721 tttctccgtt atctttttag tggagttaaa tatactggaa tcagggttta acttacccga
     3781 atacacgcgg aataaggtta acctgcctgc gtaggggtcg gcaatcgttt taaaaactat
     3841 cgccgagaaa ggagcgtctt caacaggttg tctttcttca atattgtctg ttcccggctt
     3901 cttgcctttt gccggaccac ggtcaactgg agacgaaaag gagttgacga ctaagtccag
     3961 cagcaaggaa acgccaatgc cttttattga tgatccgcag actaccggaa ttaagcttcc
     4021 cgaagaaaca ccttttttta atccggcctt tagttcttcc ggtgtaagtt cacctgcgtc
     4081 cagatactta ttcattagtt cgtcatcagc ttcagcaata tcttccacca ttgtgccacg
     4141 taagcttttt acttcatcca tcgtttctgc ggggatatcg acggctttac cgattccttt
     4201 gttgtcgcta aataaataag ctttcatgcc gattagatca ataatgccct gaaatttgtc
     4261 ttcagctcct atgggcacac aaaccggggt tacttttttg gcgaatttgc tcttgatact
     4321 ttccagagcc ttattaaagt cggcacgtac acgatccatt ctgttgataa aaataatacg
     4381 gggaagtttg aattcatcgg ctaattccca aactttttgt gtctggaatt caacaccgcc
     4441 gacagcatca acgacgatga tcgcattttc aacaacgctc atcgaacatt ttatatcaaa
     4501 ggcgaaattg gaatcgccgg gagtatcaat aatgttgatt ttgtgttttt cccattcgat
     4561 aaaatttgct gcggaactga ttgatacacg ccgtttttgt tcttccggct cataatccat
     4621 ggatgatgtt gaatcatcaa ctcttcctaa ccgttcgttt ttcccggaaa taaacaaaaa
     4681 agattcacaa agggaggttt taccagttcc accgtggccg acgactgtta aatttctcag
     4741 ggacttggat tcgtactttg ccatgaaagc tcctttcaat atcgcattac tatctttaaa
     4801 aaagcagttt cattggaatg ggcgcttgtt tagcttatcc ggcaagtaaa atcaagacaa
     4861 ttttaatctt taagcgttcc aataactgcc gggtgggatg cgggattagt tgaatattac
     4921 aagggaaaag tttatctgat ccgaagtttt atttcaccga gacgcaaggg gtaaaatatg
     4981 gcagccagac aaagaaagag agcaagacca aaggagacaa tcagccatgt tatttgcagc
     5041 aaagaaagct gttttccgtg gacgtcagcc ataaacgcga aatacaccgg accagcttcc
     5101 agaattataa ccaaggcaat taaaccaaaa caaaggatca tgaaaagcaa tccgccaaaa
     5161 ctagttacaa cctgtgccgg attttccgat ttaaaatttg ggtagacagc gcctaagcca
     5221 atagccatgg ccaccacagc aggaacaaga caaaacattg ttatggtcga tagacccatc
     5281 ataaaaggag aaacttgcag taagaaatta gatacaacaa ttagaatttc cgccaaaatt
     5341 attagaggta tgtaatacaa aaaaaactta atccagagga aggtttttat cgataccggc
     5401 gccgcctgaa cgatccaaaa ggcctctcct tccatactta ctgcgggaaa aacgaaacgc
     5461 gcagcaatgg ccgaaagcac aaaagccgcc agccctatgt tcaagaaaga taataaattt
     5521 tgcaggtaaa tggttttaat cggcgatttg tccaaaggca aaacggaaaa attgtagagg
     5581 taaattacaa ttaaggcggc catcataaat acttgcggcc attgtgtcga atcgcggaaa
     5641 aaagttcgga tttctttgat ggcaaaagct tttgcttcac gcgggagaaa atttaataaa
     5701 ctttcccagc ttaagccttt gtattttacc ggcgcaaaaa ggcgctgcgg cgttgtttgt
     5761 gatttagaga agcccataaa gtatgcagca tgggctataa tgttactgat gaaaataagc
     5821 ataaaggcac agcttccggt aagggcgata ttaaataggg agcccattat tttgccatta
     5881 agggcggttt taatggcatc tgtaatccag gttgtgggta aaaatggcga gttgggcgtt
     5941 tgcagggaat ttaaataaag aacagcagaa gcaaaactat ccggattaac caattgttcc
     6001 ggccgcatta aacgcataac taaaacgaga atcaaaacca ggaaaacgct aagaatgatt
     6061 aggattgtcc tgattcttcc agcaggcagt attgtagccc caaacaaaac tataatgccg
     6121 cttaaagccg aagctatcaa gcacataaaa attattgcca ataaaacgat catataaaaa
     6181 atgatacttg ctttgtaaat aaggccataa gataaaaatg ccggaagact gaaagcaaca
     6241 accatccaag aactgtcaaa ggtactgata agccaccggg aaagaaatat gtctttggcc
     6301 gagactggca gagaatgcaa aagcggtaaa tcctggctta aataaagatg ggaaagacaa
     6361 ttaatcatgt tgctgaagag caaaaggaca aaaaaggtca tgatgatcat cgaaagaagc
     6421 ttcattgcca gtatatcgcc gaagtcctgc acgctttgaa aataaaataa aacccggtag
     6481 agaataatga aggtgcccgc ccaaaaaaca ataccgagtg cgccgaacat aaaaatacgc
     6541 atccaactat ttctttcatt gtccatgcgt agattattga tcgtggaaag cacgcggggt
     6601 tttaataagg aaaaaatcgt attcataaaa aggcattttc cttaatt
//
LOCUS       DASVIM010000136         2478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_138626, whole genome shotgun sequence.
ACCESSION   DASVIM010000136 DASVIM010000000
VERSION     DASVIM010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138626"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..321
                     /locus_tag="VF305_02770"
     CDS             31..321
                     /locus_tag="VF305_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7416090.1"
                     /translation="MLALTQPCANKGVLKQDPHIKIKIKIEAHSDNRGTFERNMYWSQ
                     QRAKAVADYFITKGITAGRIQFKGYGDTRPVADNGTEEGRSRNRRVEIKLNY"
     gene            complement(478..993)
                     /locus_tag="VF305_02775"
     CDS             complement(478..993)
                     /locus_tag="VF305_02775"
                     /inference="COORDINATES: protein motif:HMM:NF021453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2165 domain-containing protein"
                     /protein_id="HEX7416091.1"
                     /translation="MYSTSFLFRLVKMVTVAAIGLMAFIIAFNNITDYYSNYPFVMHV
                     LKMDTIFPGSHMHYRSISNIFVFHASYILIITMEAVMAFCGIKGSWLLFKNIKSSAVV
                     FHASKNWAIAGISIGIVIWFLGFEVIGGEWFAMWQSPTWNGLAAAERMLSFLVLILIL
                     LHFKEKDLNII"
     gene            1269..1631
                     /locus_tag="VF305_02780"
     CDS             1269..1631
                     /locus_tag="VF305_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416092.1"
                     /translation="MKRKLFNAIALCCCFIFLMAILASLDGRWTGVVKTPDGNELNVI
                     YNFKTDGDKLTGTVESPSGVMSIDSGKVAGNTFSFQVTVDGNDYPHKGTIYDDSCGLD
                     IDFGGQLMHTTLVRDTTK"
     gene            2195..>2478
                     /locus_tag="VF305_02785"
     CDS             2195..>2478
                     /locus_tag="VF305_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416093.1"
                     /translation="MPGSFQNNAYGNTFATHKNMYDSGSPKKWFVTSYTGITAGYSSF
                     RGGGASFVAAPISFQLNRRLTNNLYAFAGITAAPTYFNFNRAFTNTSLNK"
BASE COUNT          836 a          436 c          478 g          728 t
ORIGIN      
        1 cttatgtctt aactaaatga cattgagcgg atgcttgcgc taacgcaacc ttgcgctaac
       61 aagggggtat taaaacagga tccccatata aaaataaaaa taaaaattga agctcattcc
      121 gataaccgcg gaacgtttga aagaaatatg tactggagtc aacaaagagc aaaagctgtt
      181 gccgactatt ttattacgaa aggtattaca gcaggcagaa tacagtttaa aggctatggc
      241 gatacacgcc cagttgcaga taatggtact gaagagggaa ggtcaaggaa caggagagtg
      301 gaaatcaaat taaattatta aggaactgcc ggttcaggtt gaatgaaaat tgtagcagta
      361 taaggcggca gatataatta taataagaac agatataagt ttgttcaagc ttataattct
      421 tctgtagctc aatagaagta ttttatccct taggcgagcc tcgcactgcc tgtattatta
      481 aattatatta aggtcctttt ctttgaaatg taataagatc aggatcaaaa caagaaaact
      541 gagcattctt tctgcagcag caagtccatt ccaggtaggg ctttgccaca tcgcaaacca
      601 ttctcctcca ataacctcaa atcctaaaaa ccaaatgaca atgccaatag aaattcctgc
      661 aattgcccag ttttttgaag catgaaatac tactgcactg ctttttatat ttttaaaaag
      721 aagccaactg cctttaatgc cacaaaaagc cattactgct tccatggtaa tgattaaaat
      781 ataggaagca tggaaaacga aaatattact aatgcttctg taatgcatgt ggcttcccgg
      841 gaaaatagta tccattttca gaacatgcat tacaaatgga taattactat agtaatctgt
      901 aatgttatta aaggcaatta taaatgccat tagcccgata gctgctacgg taaccatttt
      961 tacgagccga aataaaaatg atgtagaata catattgcca taatgttttg gaaagaatct
     1021 gcaaatattc cttttaaaaa ataatataag aaaagttgat atagattaga tgagtgttta
     1081 aaggaattgg aaagagtaag gtagaaagaa aatctcagga aaaaaattca gaaatatggg
     1141 atacgaaaca aggcggaagg tatagcaggt gggccaaatt tataaagttg ttatttgttt
     1201 tcaaattatt atgtagatta gcttttaaaa aatattccct gtttattaca tctaaataaa
     1261 attctaaaat gaaaagaaag ttattcaatg ctattgcctt atgctgctgt tttatttttt
     1321 tgatggccat attggcaagc ctggatggca gatggacagg cgttgtaaag acgcctgatg
     1381 gtaacgaact caacgttatt tataatttta aaacagacgg tgataaacta acgggcacag
     1441 tagagtcgcc ctctggtgtg atgtctatag acagcggaaa agttgccggc aatactttct
     1501 ctttccaggt tactgtagac ggcaacgact atccgcataa aggcacaatt tacgatgatt
     1561 catgcggttt ggatattgac tttggcggac aactgatgca tacaacgctg gtgagggata
     1621 caacgaagtg agcggggaga tgaaatgaat ggtaattatt ctttttgtca cagtggtgtt
     1681 ctcccgcaga ggctgcgcca gcttagtgat gacgaccatt tgcaaaaatt taattcagga
     1741 aaaaataata tttctactca aataaattaa aatgaaaaac agggataaaa gcattatcta
     1801 tttaatcctc ggattatatt caataatggt aagctggtat tacaatcatt ctattctcct
     1861 cgtaattatc cattatattt tctggccaat ctatttagta tatgaattgc tcaacggtcg
     1921 tttggctcat aatatgtgga aaataattcc tgaatcttat ttcaattaac tggcacttat
     1981 tgaatgcaga acgttttctt aaaacgaggc ttcatatcta ctcggcacaa ttaaaaattt
     2041 acttgcagat tattttagta ccttaatttc gataggttgt aagaggcaca ggaagctaag
     2101 caaaggctgc gcctcattcc tgcgccaacg gcgggggata atactcttat ttattgtaat
     2161 tgcttttgcc actgccctac aggcacaaac aactttgccg ggcagttttc aaaataacgc
     2221 ttatggcaat acctttgcca ctcacaaaaa tatgtacgac agcggctcac caaaaaaatg
     2281 gtttgtaacg agctataccg gcattactgc aggctatagt tccttccgtg gaggcggtgc
     2341 atcttttgtt gccgctccaa taagctttca gctaaaccgc aggctcacta ataacctgta
     2401 tgcgtttgca ggtataacgg cagccccgac atattttaat tttaaccgtg cctttactaa
     2461 tacaagtctt aataaaat
//
LOCUS       DASVIM010000137         5482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_480584, whole genome shotgun sequence.
ACCESSION   DASVIM010000137 DASVIM010000000
VERSION     DASVIM010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480584"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VF305_02790"
     CDS             <1..243
                     /locus_tag="VF305_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416094.1"
                     /translation="DRPLREKIYKLSIEAPPTDDTMVEKNFTDTIRRIKEKWYKEQKR
                     QVQFKLSQAQESGDKELSNKLLYEKEDLLTKEKELR"
     gene            281..2053
                     /gene="rpoD"
                     /locus_tag="VF305_02795"
     CDS             281..2053
                     /gene="rpoD"
                     /locus_tag="VF305_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX7416095.1"
                     /translation="MVKELQSDEFKKLISLGEEKGFLTYDDVNDMLPPDVTSSDQIDD
                     IIILFGEKNIDIIDTEQGEKLVVKKPAEDVVPVKFPETLALVPGAGKTGDPVKMYLRE
                     MGSVSLLSREGEVEIAKKIEEGARETMSAIFRLSVSIKEVFNIGDKLVTDEIRIKNVV
                     DNVEDEEGFVEEDEHKERVLKLIARIRLFNDRNKVLHIKLQSKTMRSKRRELLNAELE
                     KNTKYIITLCRKVRFSKKQIHRFIARLRHYTEEMERAEKVIAQYKKETGLTVAQLEKA
                     WAKMKKSKQDEKRAAKESRVSIDLLKKSQVAINEAHKKIKQIVREVEIPAATLKAVIK
                     SIEEGETKAEMAQRKLVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEY
                     QRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRYLVQELGREPI
                     PEEIANKMEYPLEKVRKVLKIAKEPISLETPIGEEEDSHLGDFIEDKKIMSPSEATIN
                     MDLAEQTRKILSTLTPREEKVLRMRFGISDRLSSLVEGEKAPVDLIETFMEMSEVIEA
                     PVLKKVKYKSKKYKSKKRAAKKVK"
     gene            2119..2194
                     /locus_tag="VF305_02800"
     tRNA            2119..2194
                     /locus_tag="VF305_02800"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2152..2154,aa:Ile,seq:cat)
     gene            2401..3120
                     /locus_tag="VF305_02805"
     CDS             2401..3120
                     /locus_tag="VF305_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX7416096.1"
                     /translation="MKENLSLLIQLQECDSHLVKLSAKKKNLPGTIDKLDKNFNSFKE
                     GIEQNKIKYDELKSRHTESESKIKKLNEGMVKTKERLLEVKNNKEYQAMLKENEVAES
                     ACGDIETEIISILEELDKLSVLVKKDEETLKRSKDKYEQEKKIIEDDLNTIDTDVVSW
                     EKKRIDLRKNVPDDLLARYEKVKDRNKGVGVISVWKEVCNGCHMNIPPQLYNELQKSN
                     ELLSCPNCNRIIYFQSMEKPL"
     gene            3126..3533
                     /locus_tag="VF305_02810"
     CDS             3126..3533
                     /locus_tag="VF305_02810"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX7416097.1"
                     /translation="MEEKIYKLFSDGACRGNPGVGGAGAVITDARENVVWAGKEYLGQ
                     CTNNIAEYRALILGLKGALAHSYKSLEVYLDSELLTKQINGSYRVKNENLKVLMKEVR
                     SLLTSFESVQVKHVLRSRNSHADKLANLAIDEK"
     gene            complement(3683..>5482)
                     /locus_tag="VF305_02815"
     CDS             complement(3683..>5482)
                     /locus_tag="VF305_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX7416098.1"
                     /translation="EEAFKALGAHSYIAILIGLATAFTVFIIAYAYMRIIEHFPQGGG
                     GYIVSTKTISNSAGVISGSALIVDYMLTITVSLVACGDAIFSFLPVTYQKYKLLFVAF
                     LIFALIIMNLRGVKESVMILAPIFVVFVLTHILLIGYGLFTHVDQVGTVFSQFNSSFH
                     QDITAIGFIGILAIFLRAFSLGGGTYTGIEAVSNGMQIMREPKVQTGKRTMVYMAISL
                     AFTAAGLLICYALFQIKPAEGRTLNAILSSEIFSNWPLGAWIALITILSEGALLFVAA
                     QAGFVDGPRVMANMATDYWLPHRFSMLSERLTMQNGILLMGGASIILLFYTHGSISAL
                     VVMYSINVFLTFSLSQFGMIRFFLHDKEKDKNWKRHIIIHVIGFILCVTILCVTVYEK
                     FGEGGWATLVITSVLIGSCYLIKRHYHKVRQAALQLEDILQKIRPGKNFNNEPLNPKE
                     PTAIMLVSGFNGFGLHTWLSINRKFPHFYKNFIFVSIAEVDQEVFKGTAEMQQLTTSV
                     EDGLKKYVHLVRSYGMAADYRIDTGTDVVEKATELCESLAKEFHKSMVFTGKLIFRQE
                     HLFQRLLHNETAYAIQRRLQWDGIETVILPIRV"
BASE COUNT         1868 a         1025 c         1230 g         1359 t
ORIGIN      
        1 gatagaccct tgcgcgaaaa gatatataaa ttaagtattg aggcaccacc gacagatgat
       61 acaatggtgg aaaaaaactt tactgatact ataagaagaa ttaaagaaaa atggtataag
      121 gaacaaaagc gccaggtcca gttcaagttg agtcaagcac aagaaagtgg tgataaagag
      181 ctgtccaata agcttcttta tgaaaaagaa gacctactga caaaagagaa agaattacgt
      241 taaacaagct gagcaaataa aatatttggg gagatgccaa atggtgaaag aactgcaatc
      301 tgatgaattt aagaaattaa tttcactggg cgaagaaaag ggttttttaa cttacgatga
      361 tgtcaatgac atgttgccgc cggacgtaac ctcttcggat cagattgatg atattatcat
      421 acttttcgga gaaaagaata ttgatatcat tgataccgaa caaggcgaaa agctggtggt
      481 gaaaaagccg gcggaagacg ttgtgcctgt taaatttccg gaaacactgg cgcttgtacc
      541 cggtgcgggc aaaaccggag atccggtgaa aatgtacttg cgcgagatgg gatcggtttc
      601 tcttttgagc cgcgaagggg aagtggaaat agccaaaaaa atcgaagaag gcgcgcgcga
      661 gacaatgtcg gctatcttta ggctatccgt ttcgattaaa gaagtgttca atattggcga
      721 taagttggta accgatgaaa ttcggatcaa gaacgttgtt gacaatgttg aagatgaaga
      781 aggctttgtg gaagaggatg aacacaaaga aagagttcta aaactgatcg ccagaattag
      841 actttttaac gatagaaata aagttttgca cattaaactc caatctaaga ctatgcgcag
      901 taagcgacgc gagttattaa acgccgaact ggagaaaaac acaaaataca tcattacgct
      961 ttgccggaaa gttcgtttca gcaaaaaaca aattcatcga tttatcgccc gtctgcggca
     1021 ttataccgag gaaatggaaa gagccgaaaa agttattgct caatataaaa aggaaaccgg
     1081 tttaaccgtt gcgcaattgg aaaaagcctg ggcgaaaatg aaaaaatcca agcaagatga
     1141 aaaacgcgcg gcaaaagaat ctcgtgtttc cattgatctg ctcaaaaaaa gtcaagtagc
     1201 aataaatgaa gcgcataaaa agataaagca aattgtgaga gaagtggaga taccggcagc
     1261 aactttaaag gcagttatta agtccattga agaaggtgaa actaaagcgg agatggccca
     1321 aagaaagctc gtggaagcaa atttgcggct ggttgtgagt attgccaaga aatacactaa
     1381 ccgcggtttg cagtttcttg atttgattca ggaaggcaat atcggtctga tgaaggccgt
     1441 ggataaattt gagtatcaac gcggctataa attttctact tatgcgacat ggtggatcag
     1501 acaggcgatt acacgcgcta ttgcagatca agcgcggaca attcgaattc ccgtccacat
     1561 gattgaaacg atcaacaaac tgattcgcac atcccgctat ctagtgcagg aactgggtcg
     1621 tgagcccatt cccgaagaaa ttgccaataa aatggaatat ccgctggaga aagtcagaaa
     1681 agtattaaaa attgccaaag aaccgatttc tttggaaact cccatcggcg aggaagaaga
     1741 cagtcattta ggtgatttta tcgaagacaa aaaaatcatg tctccttcgg aagcaacgat
     1801 caatatggac ttggcggagc aaacgcgaaa aatattatca acactgactc cgcgcgagga
     1861 aaaagttttg cgaatgcgct ttggcatcag cgaccggtta agttctttgg tcgaaggaga
     1921 gaaagcgcct gtggatttaa ttgaaacatt tatggagatg tctgaagtta ttgaagcgcc
     1981 ggtgttaaaa aaagtgaagt acaaatcaaa gaagtacaaa tcaaagaaac gtgccgcaaa
     2041 aaaagtaaaa tagtattgac aaggtgtata tttgtgtata tcaaggcaag ccctaataca
     2101 caccttaaaa aaaatggcgg gcccatagct caattggtag agccaccggc tcataaccgg
     2161 taggtccctg gttcgatccc aggtgggccc accaaattat ggaaaacaac aatgataaga
     2221 aaaaatttat ttttctcccc caggagtaga tattcgacag ccattgtttt aactgtcgtg
     2281 gtgagacgaa caatgtcatc attacacggc acagataata aatttatgaa gcagtccact
     2341 taaaaaatgc acccctaaaa ggtgcatttt ttttatccta ataaaagaag gagaagatta
     2401 ttgaaagaga atttatcatt attaatccag cttcaggagt gtgattcgca tttagttaag
     2461 ttgtccgcca agaaaaaaaa tctgccggga acaatagata aactggataa aaactttaac
     2521 tcatttaaag aaggaatcga acaaaacaaa ataaagtatg atgaactcaa atcccgccac
     2581 actgaaagtg aaagcaagat taaaaaactc aacgaaggca tggtaaaaac aaaagaaaga
     2641 ctgctggaag ttaagaacaa taaggaatac caggccatgc tcaaggaaaa cgaggttgcc
     2701 gaatcagcgt gcggcgacat tgaaaccgaa ataatttcga tattggaaga gttggataag
     2761 ctttccgtat tagtgaaaaa agatgaggaa actttgaagc ggagcaagga taaatacgaa
     2821 caggaaaaga aaataatcga ggatgatctg aacacaatag ataccgatgt cgtgagttgg
     2881 gagaagaagc gcattgatct gagaaagaat gttcctgatg atctcttggc tagatacgaa
     2941 aaggttaaag acagaaacaa aggtgttggc gtaatttctg tttggaaaga agtttgcaat
     3001 ggctgccaca tgaatattcc gccgcaactt tataatgaat tgcagaagtc gaatgagtta
     3061 cttagttgtc ccaattgtaa tcggataatt tactttcaaa gtatggaaaa gccgctttag
     3121 tctctatgga agagaaaatt tataaattat tcagcgacgg cgcttgccgt ggcaatcccg
     3181 gtgtcggtgg cgccggcgca gtcataaccg atgcccggga aaatgttgtt tgggcgggca
     3241 aggaatatct aggccaatgc accaataaca ttgctgaata tagagcgctc attttgggat
     3301 taaagggtgc tttggctcac agctataaga gtctggaagt ttatttggat tcagagcttc
     3361 tgaccaaaca aattaatggt tcttaccgcg ttaaaaatga aaatttgaag gtcttgatga
     3421 aagaagtccg cagcctgctg acttcttttg aatctgttca agttaaacac gtcctgcgtt
     3481 cccgcaacag ccatgctgat aaactggcca atctggcaat tgatgaaaaa taattatgga
     3541 gacatcaagg gggaaagagg agcacgtgga atggaccatg gacaaggaga aaggagatag
     3601 taattaattg aggtaaagta gaaaaaataa ctgaatgata ttaacgccgc tttttttcct
     3661 ctttccatta aaattatttg tatcaaacgc gaatcggcaa aatgacggtt tctattccat
     3721 cccattgtaa acggcgttga atcgcgtaag ccgtttcgtt atgaagcagt ctctgaaaca
     3781 aatgttcctg gcggaagatc aatttcccgg tgaaaaccat ggacttatgg aattcttttg
     3841 ccagcgattc acacaactca gtagcttttt ccaccacatc cgtcccggta tcaattctat
     3901 aatctgcggc cattccgtaa gagcggacaa ggtgtacata ttttttaagg ccgtcttcta
     3961 ccgaagtagt taattgttgc atttctgcag ttcccttaaa aacttcctga tcaacttcgg
     4021 caatcgatac aaaaataaag tttttataaa aatgcgggaa ttttcggtta atcgaaagcc
     4081 acgtatgcag gccaaatccg ttgaaaccgc taacaagcat gattgccgtt ggttcctttg
     4141 gattcaatgg ttcgttatta aaatttttgc ccggacgtat cttttgtaga atatcttcca
     4201 gttggagtgc tgcctgtcgg actttatgat aatggcgttt aattaaatag catgaaccta
     4261 tgagaacgga tgttattact aatgtagccc agccgccttc cccaaatttt tcataaacag
     4321 ttacgcaaag tatcgtcacg cagagaataa aaccaattac atgtataatt atgtgtcttt
     4381 tccaattttt atctttttct ttatcatgaa gaaaaaagcg gatcatgccg aactgagaga
     4441 gtgaaaatgt tagaaaaaca ttgatcgagt acatgacaac aagggcggaa atggaaccat
     4501 gtgtataaaa caaaagtata attgatgcgc cccccattaa taagatgcca ttttgcatcg
     4561 tgagccgctc cgagagcatg gagaagcgat gcggaagcca ataatcagtt gccatattgg
     4621 ccataactcg tggcccatca acaaagcctg cttgagcagc tacaaaaagc aacgcgcctt
     4681 cagaaaggat cgttatcagc gcaatccacg ctcccaaagg ccaattgctg aatatttcgc
     4741 tggagagaat cgcgtttagt gttctgcctt cagccggttt tatctgaaaa agtgcataac
     4801 aaatcaataa accggcggct gtgaaggcga gcgatattgc catgtaaacc atcgtcctct
     4861 ttcccgtctg aacttttggc tctcgcatga tctgcattcc gttggataca gcttctattc
     4921 ctgtatatgt cccacctccc agagagaagg cccgcaaaaa aattgccagt atgccgataa
     4981 agccgattgc tgttatatcc tgatggaaac tgctgttaaa ctggctaaag acagtcccaa
     5041 cttgatcgac atgagtaaat agaccataac caatcagcaa aatatgggtc agaacaaaaa
     5101 caacaaatat gggcgcaaga atcatgacgg attccttgac gccccgcaaa ttcatgatga
     5161 tgagtgcaaa aataagaaaa gcgacgaata gcagtttgta tttttgataa gtaaccggca
     5221 gaaaactgaa aatggcatcg ccgcaggcga ccagggaaac cgtgattgtc agcatatagt
     5281 caacgatcaa tgcgcttccc gatattacgc cggcgctatt gctgatggtt tttgtcgaaa
     5341 caatataccc gccgccgcct tgcgggaaat gttcaattat gcgcatgtag gcataagcaa
     5401 taataaaaac agtaaacgcc gtggcgagac cgataagaat tgcaatatag gaatgagcgc
     5461 caagagcctt gaatgcttct tc
//
LOCUS       DASVIM010000138         4043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_310573, whole genome shotgun sequence.
ACCESSION   DASVIM010000138 DASVIM010000000
VERSION     DASVIM010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310573"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VF305_02820"
     CDS             <1..729
                     /locus_tag="VF305_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX7416099.1"
                     /translation="PTGSGKSTSLYAILNSINKPDINIITLEDPVEYRINNIRQAQLN
                     RKAGMTFASGLRSILRQDPDVIMVGEIRDAETAAISVQAAQTGHRVLSTVHTNDAAGA
                     ITRFIDMGIEPFLISSVMLVSFAQRLVRTVCPYCKESYTPPEKALAAFGITTAEAKNA
                     NFQRGKGCYQCKNTGYKGRTGLFEVLVNDEMVQDMIIKKKPSQEIARAAVDAGKLRTL
                     REDAAQKVMQGITTLEEAASAVMT"
     gene            749..1978
                     /locus_tag="VF305_02825"
     CDS             749..1978
                     /locus_tag="VF305_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7416100.1"
                     /translation="MPNYIYQAINENGNTVSGTLETESVEAANSILSSRGLIPLKINQ
                     EQGKSGDNMWSQIISATSTVKVSDLIIFTKQFRSMLGAGVPILRLLQVLEAQTESRGL
                     RKALSTIIQDIRQGSSLSEALEKYPKIFSRLYCAMIKAGEISGNVPGVLDRLIYIIEH
                     ETKVKADIKSALRYPLMVVIALGIAFIVLLTFVVPKFATLFSKAGLVLPWPTQFAMFL
                     YSFLVNYWYLILTVIVIIIFSLVSYFKTSQGRFVRDSFLLELPVIGPLFKKAALSRFA
                     SIFAILQTSGVPVMQSLTILSATIGNEAIARAFEYVRERIEEGAGISTPLKSAKYFTP
                     MVIDMIAIGEESGNIDEMLREITKHYDDEVEYAVKGLSDAIGPILIVGLAAVVGFFAL
                     AIFMPIWDLTKLATQQH"
     gene            1942..3312
                     /locus_tag="VF305_02830"
     CDS             1942..3312
                     /locus_tag="VF305_02830"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7416101.1"
                     /translation="MGSYKIGDTTTLKYKKMSEKKFYVNLYILIPVIYAGISIIGVIV
                     TYQLLSPNEKISPISSLGFVYLIAAIGFFTFFISFVMLRVLLKPFMLFVHKTQKLPIF
                     TNSPLGKQEKITDDLDQINKVFDNVANILSHVEAKELFPHVIGSSASMRNIFTQIIKV
                     APTDSTVLIFGESGTGKELIASSIYEHSLRKGRPFVKINCVAIPEGLLESELFGHEKG
                     SFTGATGQKKGKFEIADGGTIFLDEIGDMPIATQAKLLRVLQEKEFERVGGTKPLRVD
                     VRFIAATNKNLLKIIKEGSFREDLYFRLNVFSISVPALRERREDISIIANYFLESLPK
                     PAKLSTSALQILIGYSWPGNVRELRNVLEQASILSENGVIELQHLPEALLKQNPLSMV
                     NNFNDDIPLDDKLELMEKEIIMAALRKTGGIQVKAANILRINQRSLWHRIKKLNIDIN
                     MLRNLQ"
     gene            3613..4017
                     /locus_tag="VF305_02835"
     CDS             3613..4017
                     /locus_tag="VF305_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7416102.1"
                     /translation="MLRNEKGFTLIEIIAVLVILGILAAVAVPRYLDLMGQARQLAAQ
                     GAIAEVKGRLSSAQAKYMMANSGNAPTAAQLLVYAQDATNGYGANLVNVGTDFTIAPL
                     VSASPIVITVNAVQGTTLSPVVNGNFLTANGT"
BASE COUNT         1222 a          748 c          918 g         1155 t
ORIGIN      
        1 cccacgggaa gcggaaaaag caccagtctt tacgcaatct taaattcgat aaacaaaccg
       61 gacataaata taatcacgct ggaagatcct gtcgaataca ggatcaataa tattcgccag
      121 gcgcagttaa atcgcaaagc agggatgacc tttgccagcg gtttgcgttc aattcttcgt
      181 caggacccgg atgtcattat ggttggtgaa attagagatg cggaaactgc cgcgatatct
      241 gtgcaagcgg cgcaaacggg acatagagtt ttaagcacag tgcataccaa cgatgccgcg
      301 ggagctataa cgcggtttat tgatatgggc attgagcctt tcttgatttc atcggttatg
      361 ctggtatctt ttgcccaaag attagtgcgt actgtttgcc cctattgcaa agaatcttat
      421 actccgccgg aaaaggccct ggctgctttt ggaataacaa cggcagaagc aaaaaacgct
      481 aattttcagc gtggtaaagg ctgttaccag tgcaagaaca cgggttacaa aggaagaacc
      541 ggtctttttg aagttttagt aaatgacgaa atggttcaag acatgattat caagaaaaag
      601 ccgagtcagg aaattgcccg cgcggcggta gatgccggaa agttgagaac cctgagagaa
      661 gatgcggccc aaaaagtaat gcaaggaatt acaaccctgg aagaagcggc ttccgccgtt
      721 atgacttaga ctaaattaag gacgaaaaat gcccaactat atttatcagg caattaacga
      781 aaatgggaat acagtgtcgg ggactttaga aactgagtcc gtggaagcgg caaattccat
      841 attatcttca cgtggcctta taccgttaaa aattaatcag gaacagggta aatccggaga
      901 taatatgtgg tcgcagatta tttccgcgac gagcacagtt aaggtgtcgg acctgatcat
      961 ttttacaaag cagttccgtt ctatgctggg cgccggtgtg ccgatattaa gacttctgca
     1021 ggttcttgag gctcagacag aaagccgggg cttgaggaaa gcattatcaa caattattca
     1081 agatatcagg cagggttcgt ctctttcgga ggcattagag aaatatccca aaatattttc
     1141 acgtctttac tgcgctatga tcaaagcagg agagataagt ggaaatgtgc ccggcgtttt
     1201 agatcgtttg atctacatta tcgaacatga aacaaaagtc aaagcagata ttaagtcggc
     1261 attgagatac ccgctaatgg ttgttattgc tttaggcatt gcctttatcg tacttttaac
     1321 ttttgtcgtg ccaaaatttg ctactctttt tagcaaagcc ggtttagttt taccgtggcc
     1381 aacacaattt gccatgtttt tgtactcttt tctggttaat tactggtatt tgattcttac
     1441 cgtaatagta ataattattt tcagcctggt gtcctatttt aagactagtc agggcaggtt
     1501 tgtccgggat tcttttttat tagaacttcc tgtaattggg ccattattta aaaaggcggc
     1561 attatcgcgt tttgccagta tttttgctat tttgcaaacc agcggcgttc ccgttatgca
     1621 atcattgact attctttcgg caacaattgg taatgaggct attgcgcggg cttttgagta
     1681 tgtccgtgag cgtattgaag aaggtgcggg tatttctaca cccttaaaat cagcaaagta
     1741 ttttacaccg atggttattg atatgatcgc gattggcgaa gaatcaggta atattgatga
     1801 aatgctgcgc gaaattacca aacactatga tgatgaagtt gagtatgcgg tcaaaggtct
     1861 ttcggacgcc ataggcccga ttcttatcgt gggtttagcc gcagttgtcg gcttctttgc
     1921 cctcgccata tttatgccga tatgggatct tacaaaattg gcgacacaac aacattaaaa
     1981 tataaaaaaa tgagcgaaaa aaaattttat gttaatcttt atatattgat cccggtaata
     2041 tatgcgggaa tatcgataat cggcgttatc gtcacgtatc aattgctcag tcccaatgaa
     2101 aaaatcagcc cgatatcctc acttggtttt gtatatttaa tcgccgccat agggtttttt
     2161 acttttttta taagttttgt catgttacgc gttcttttaa aacccttcat gctatttgtt
     2221 cataaaacgc agaagctgcc gatttttacc aattcccctt tgggaaaaca ggaaaaaata
     2281 accgacgatt tagatcagat taataaagtg tttgataatg tggcgaatat tctaagccac
     2341 gttgaagcaa aggaattatt tcctcatgtt attggttcca gcgcctctat gcgtaatatt
     2401 tttacgcaga ttattaaagt tgcgccaacc gattccacgg ttcttatttt cggtgaaagt
     2461 gggaccggca aggaattgat tgcctccagt atttacgagc atagtttgag gaaaggcaga
     2521 ccttttgtga aaattaattg cgtggcaatt ccggaaggat tgctcgaaag cgaacttttc
     2581 ggccatgaaa aaggttcgtt taccggcgct accgggcaaa aaaaaggcaa atttgaaata
     2641 gctgacggtg ggactatttt tttagatgaa attggggata tgcctatagc tacacaggcg
     2701 aaacttttac gggtattaca ggagaaagaa tttgagaggg taggcggaac aaagccgctt
     2761 cgggttgatg tccgctttat tgcggctaca aataaaaatt tattaaagat aatcaaagag
     2821 ggatcattta gagaagacct ttatttccgg ttaaatgttt tttccatctc cgttcctgcg
     2881 ctccgggaga gaagagagga tatttccatc attgccaatt atttcctgga gtctttgccc
     2941 aaaccggcaa aattgtccac ttccgcgctg caaatattaa ttggttactc ttggccggga
     3001 aacgtcaggg agttgagaaa tgttttagag caagcttcca tattgagtga aaacggcgtt
     3061 attgagttgc agcatctgcc ggaagcgcta ttgaagcaaa atccattaag catggttaat
     3121 aattttaatg acgatattcc tctggacgat aagctcgagt taatggagaa agagataatc
     3181 atggcagctt taagaaaaac cggagggatt caagtcaaag cagcaaatat attgagaata
     3241 aaccaaagaa gcctttggca tagaattaag aagctcaata ttgatataaa catgctcagg
     3301 aatctacaat aattgtagat aaaacgacaa tatttgtaat ttagagataa gtacagggga
     3361 ggctttctgt aaaaaaggat tgacaaaaca aaaaagttaa gttaattaaa cgtgttagaa
     3421 aggtataagg tggaaggcag aaattgcata gagccctaat aatggcacgg cacttgcttt
     3481 tagacctaca tacataatta gatctatata cataaatgct ataaatgatt ttcgaaaaaa
     3541 aagcgtgatc aggtaatcaa agaaattttg attggcaatt aacaatttac aattaaacaa
     3601 ggaggaaaga atatgttaag aaatgaaaaa ggttttacat tgattgaaat tattgcagtg
     3661 ttggttattc taggtatttt ggcggcggtg gctgttccca ggtacctcga ccttatgggc
     3721 caggcgcgcc aattggccgc tcagggtgcc attgccgaag tcaaaggcag gctgtcttcc
     3781 gcacaggcca aatacatgat ggccaatagc ggtaacgctc cgaccgcagc acagttgctt
     3841 gtttacgctc aggatgctac caacggatat ggtgccaatc tggtaaatgt tggtaccgac
     3901 ttcaccatag caccattagt aagtgcttcg ccgattgtta tcactgtaaa tgcagttcag
     3961 ggtacaaccc ttagccctgt tgtcaatggt aacttcctca cggcaaatgg aacttaacgg
     4021 tagaggacgg gattaatagt tct
//
LOCUS       DASVIM010000139         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1505930, whole genome shotgun sequence.
ACCESSION   DASVIM010000139 DASVIM010000000
VERSION     DASVIM010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505930"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..139)
                     /locus_tag="VF305_02840"
     tRNA            complement(53..139)
                     /locus_tag="VF305_02840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Leu,seq:tag)
     gene            409..1404
                     /locus_tag="VF305_02845"
     CDS             409..1404
                     /locus_tag="VF305_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7416103.1"
                     /translation="MEAKSISVLLLEDNPGDARLIRELLADTNDKFELQCVDLLSKGL
                     DCLASQTIDAVLLDLGLPDSQGLDTIESVRAKKSSIPIIVLTGLNDETVAIEAVRRGA
                     QDYLIKGSSTGNMLSRVIRYAIERKNTEQELKQSLEKFTRALSGTVKAMAMTVEIRDP
                     YTAGHQRRGADLAYTIAEEMNLAPERVEGVRLAGMIHDIGKISIPAEILSKPSLLSNL
                     EFSLIKIHPQSGYDILKEIDFPWPIAKTVLQHHERINGSGYPADLKNDDILLEAKVLA
                     VADVVEVIASHRPYRPAFEIQKAIDEITQNQGILYDPAVVKSCLRLFQDKEYSFS"
BASE COUNT          475 a          301 c          294 g          458 t
ORIGIN      
        1 tcactgaagc ctatctcctt tgtttttgaa atattataaa tatgcgtgaa cttggtgcga
       61 gagaggggat ttgaacccct atccgcgtag gcgggctgga tcctaagtcc agtgcgtctt
      121 ccaattccgc cactctcgcc tttagatttt cttgaattat atattcatta cgcctgaaaa
      181 tgtcaacgcg gattattttc tgcacacaca aacaaaacta gttaaattaa gctttttgtg
      241 agttcattta tttgataatc ttcttttatg caagaaaaat gtcttaatta atcgttggca
      301 cgtaatgatc aattaatttt ttattctata actatatttt ttgactttac ttaactttca
      361 gggtataaaa aaaaagttaa aataaaaaaa ggattctcca cttcgaaaat ggaagctaaa
      421 tcgatcagcg ttcttttgct cgaagataac cccggtgatg ctagattaat tcgcgaactg
      481 cttgctgata ccaacgataa atttgaactc cagtgtgttg atcttttatc taaaggtttg
      541 gactgtcttg cttcgcaaac aatcgacgct gtgcttttgg atttaggatt gccggatagc
      601 caaggcctcg ataccatcga aagcgtacga gctaaaaaat cttccattcc gataattgta
      661 ttaactggtc ttaatgacga aactgtagcc attgaagctg tgcgccgggg agctcaagac
      721 tacttaatca aaggctcttc cacgggcaac atgttatctc gcgttattcg ttatgctata
      781 gaaaggaaaa ataccgagca ggaattaaag caaagcctag agaaatttac cagagcttta
      841 agcggcacag taaaggcaat ggccatgact gtggaaatta gggatccata cacagcaggt
      901 caccagagac gcggtgctga tttggcttat accatagccg aagaaatgaa tcttgcccca
      961 gaaagagtcg aaggagtccg ccttgccggc atgattcatg acatcggtaa aatttccatt
     1021 ccggctgaaa ttcttagtaa accctcgtta ctttccaact tagagtttag cttgataaaa
     1081 attcaccccc agtcaggata tgatatcctc aaggagattg atttcccctg gcctatcgcc
     1141 aaaaccgttt tacaacatca tgaaagaatc aatggcagtg gttatcctgc cgatttaaaa
     1201 aatgatgata ttttattgga agcaaaagtt cttgccgtcg ctgatgtagt cgaagtaata
     1261 gcatcacacc gtccttatcg ccccgctttt gaaattcaaa aagctataga tgaaatcacc
     1321 cagaatcaag gtattcttta tgatcctgct gttgtgaaaa gttgcttaag gttatttcaa
     1381 gacaaggaat attctttcag ttgactttta tcttatggct aaaaaactaa cctatcaaga
     1441 actattaaaa aaaattgatc aactggaaaa gaagtgtgct caatatgctt ccgtcgatga
     1501 acaatatcgc aaagcggaaa gtgtttat
//
LOCUS       DASVIM010000140         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1080752, whole genome shotgun sequence.
ACCESSION   DASVIM010000140 DASVIM010000000
VERSION     DASVIM010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080752"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VF305_02850"
     CDS             complement(<1..245)
                     /locus_tag="VF305_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416104.1"
                     /translation="MTSKIKKRFWFDFSVWVVIGAIAVMAIVFAVMALAHFQRQKEQA
                     VELLIEKGATLIRSFEAGLRSSLSIKEGRFGLQKLLM"
     gene            678..1178
                     /locus_tag="VF305_02855"
     CDS             678..1178
                     /locus_tag="VF305_02855"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX7416105.1"
                     /translation="MKKLTLVLMIAALGVLLTLPAFAACTGDCQGNCPGGDPGYHRDK
                     IFKKLNLSDEQKTKVEALISENQKAVRPLREKMFDKSVELRRLWLQANPDKDKITAVQ
                     KELRTLRDKKEDRATALRFEIRKVLTPEQNEKLANSRWDRGPGFGPRGGMRGHEEHGS
                     GMGNCQ"
     gene            1268..>1781
                     /locus_tag="VF305_02860"
     CDS             1268..>1781
                     /locus_tag="VF305_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX7416106.1"
                     /translation="MKLNNHIIINEHAAGQKKYWKGLLLLPLITLLILLLFISSNYGE
                     AAEINLIENSYGENKPLAPRILVAYATRAGSTVGVADAIGKKLAAGGAMVDVKPVEKV
                     QSIEGYQSVVLGSAIRFGTPLPEMTAFVHTHKDELHKVPAAYFIVCFILRENTEENRK
                     TANTYLDVLRA"
BASE COUNT          550 a          368 c          368 g          495 t
ORIGIN      
        1 tccattaata atttttgcag cccgaatctt ccctctttta tgcttaggga actgcgcagg
       61 ccggcttcaa atgaccggat cagcgtcgcg cccttctcta ttaaaagttc gactgcctgt
      121 tctttttgcc gctgaaaatg cgccaaagcc attaccgcaa acacaatggc cattacggct
      181 atagcgccaa ttactaccca aacagaaaaa tcaaaccaga atcttttctt aattttactt
      241 gtcatatcca aatctccagt agttgctgga tattctgtat ccagttggat acttttgttg
      301 gataaatatt atccacttat ttccgaaata tttcaatgtg aaattgatac attcgttatt
      361 aatctcgtga ttacggactg ttggcaaata aaagcatcat tggcataccc cttgctctac
      421 aacatgttaa ctgaaacaaa gataaaagag taaggaggtt gattatgaaa aagttaacat
      481 ggacattgat agcatgctta atcgcttttc tattgaacag atattatccc ttcgcttcca
      541 gaaaattaat attggaatat atatttgttg cctatttatc tttcaaattc agcaagttaa
      601 tgaatattga ggctgtttat aaatcacatt gcttagaagc gattaactaa attaaccctc
      661 tggaaaggag attgattatg aaaaagttaa cattagtatt aatgattgct gcactgggag
      721 tacttttaac gctgcctgcg tttgctgcct gtaccggcga ttgccagggt aattgtccgg
      781 gaggcgaccc tggttatcat cgggacaaaa tatttaaaaa attgaatctt tccgatgagc
      841 agaagacaaa agttgaagca ctgatatctg agaatcaaaa agcagtaagg ccgctgaggg
      901 aaaaaatgtt cgacaagtcg gtggaattgc gcagactgtg gctgcaggcc aatcctgata
      961 aggataaaat taccgccgtt caaaaagagt taagaacatt gcgtgataag aaagaagaca
     1021 gagctactgc acttcgtttt gaaattcgca aagtactcac cccggaacaa aacgaaaaac
     1081 tggcaaattc tcgttgggac agaggtcccg gattcggacc gcgcggcggg atgagaggac
     1141 acgaagaaca tggatcagga atgggtaact gtcaatagct gaaagaatgt cccatccctc
     1201 ctttggaagg ggagcatctg ctcccctttt tttttacatt taatcaaaaa tagtttataa
     1261 tataccaatg aaactaaaca atcacataat catcaatgag catgcagccg gtcaaaagaa
     1321 atactggaaa ggcttgttac ttcttccgct aatcactctt ttgattctac ttttattcat
     1381 ctcttctaat tatggcgaag ctgcggagat aaacctgatt gaaaatagct acggcgaaaa
     1441 taaacctctg gcccccagaa tattagttgc ttacgccact cgtgccggtt ctacagtagg
     1501 ggttgccgat gccatcggga aaaaactcgc cgcaggcggg gccatggtag atgttaaacc
     1561 cgtagaaaaa gtgcaaagta tcgaaggtta ccaaagcgtg gttttgggta gcgctattcg
     1621 cttcggcacc cctttgccgg agatgaccgc ttttgttcac acccataaag atgagctgca
     1681 caaagtgcct gccgcgtatt ttatagtttg ttttatttta agagagaata ccgaagaaaa
     1741 ccgcaaaacc gccaatacct atctcgatgt gctacgagcc g
//
LOCUS       DASVIM010000141         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_227900, whole genome shotgun sequence.
ACCESSION   DASVIM010000141 DASVIM010000000
VERSION     DASVIM010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227900"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..417)
                     /locus_tag="VF305_02865"
     CDS             complement(67..417)
                     /locus_tag="VF305_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416107.1"
                     /translation="MKVIVINYSGNVGKSTVTRYLLSPRMNNSKVITIESINSDGTED
                     DVMRGKQFTELLELLAPLDDAVVDVGASNAEDFVYMMGKHQGAHEDFNYFVVPAVKGK
                     KQTTDTITTIKSLV"
     gene            514..1737
                     /locus_tag="VF305_02870"
     CDS             514..1737
                     /locus_tag="VF305_02870"
                     /inference="COORDINATES: protein motif:HMM:NF033519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEX7416108.1"
                     /translation="MIEPYSKDIEAQMQELYGRLPEKNRRLYAGVEALKFPYGGISYI
                     AGLFGCSRDTVSAGIKELAEAETLPKNRNRKAGGGRKLTLEKEPDINEVFLILLKDHT
                     AGDPMDERRKWTNLTCAKIGSLLAEKGFKVSRNIVRKLLKKNDYVKRKALKKKAAGGH
                     VNRNAQFERIAELRDLYTAAGNPVVSVDSKKKKQIGNLFRDGKVYTTETVEVFDHDFP
                     SLAEGVAVPHTLYDIERNEAYVNIGTSRDTSEFSCDSIRHWWYEYGILYYPLATSILM
                     LMDGGGSNSSRHYIFKQDLQALVEEIGIEIRIAHYPPYTSKWNPIEHRVFPHITRELS
                     GMILTSHLFMKELIENTTTKAGLKVFACIFNRVYETGRKVVEGFKESMRILFDKHLGQ
                     WNYVAIPETTISEVA"
     gene            complement(1992..>2103)
                     /locus_tag="VF305_02875"
     CDS             complement(1992..>2103)
                     /locus_tag="VF305_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011145659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX7416109.1"
                     /translation="GYFCATVGQMTEEMIKQYLEHHFEPNPNDEFKMEPP"
BASE COUNT          634 a          403 c          457 g          609 t
ORIGIN      
        1 ttaaaggcgt tttgttacga tttttgtgca tttttgaggg gttttggaaa attaatttct
       61 gaaagtctat accagtgatt ttattgtcgt gatcgtatcg gtggtttgtt tctttccttt
      121 aaccgctggc acgacaaaat aattgaagtc ttcatgtgcc ccctggtgct ttcccatcat
      181 ataaacaaag tcctcggcgt tcgatgcgcc tacgtcaact actgcatcat cgagtggagc
      241 taacaactca agtaattcag taaattgttt gccccgcatc acatcatctt ccgtaccgtc
      301 agaattaata ctttcgatcg tgatcacttt ggaattattc atgcgcggcg acaataagta
      361 tctggtaacg gtactttttc cgacattccc agaataatta atcactatta ctttcattgt
      421 tcttcaccct taggaatgag cataaattaa tttaggcaag ttcagttcaa gaattatact
      481 ttgtgagaaa gaaaccttgt tcaaaattga caaatgatag aaccttactc caaagatatt
      541 gaagcgcaga tgcaagaatt gtatggacgt ctgccggaga aaaatagacg tttatatgca
      601 ggggtcgaag ccttaaagtt tccctacggc ggcatcagtt atatcgccgg actttttggc
      661 tgctcacgag ataccgtttc ggcggggatt aaagaacttg ccgaagcaga aactctgcct
      721 aaaaaccgca ataggaaagc aggtggaggc aggaagctta cgcttgaaaa ggaacctgat
      781 attaatgagg ttttcctgat tttattaaaa gatcatacag caggcgaccc gatggatgaa
      841 aggagaaaat ggacaaattt gacgtgtgcc aaaattggtt ctctattggc tgaaaagggt
      901 tttaaagtaa gtcgcaatat tgtcagaaaa ttattaaaaa agaatgacta tgtgaagcgc
      961 aaggccttga aaaagaaggc ggctggcggg catgtaaacc gcaatgccca gtttgagcgt
     1021 atcgctgaat tgagagattt atatactgct gcgggcaatc ctgtcgtcag cgtggatagc
     1081 aaaaaaaaga aacagatagg caatctgttt cgcgatggta aggtctatac aactgaaact
     1141 gttgaggttt ttgatcatga tttcccttca ttagccgaag gcgtcgccgt gcctcatacg
     1201 ctttatgaca tagagcgcaa cgaagcctat gtcaacattg ggacgagtcg tgacaccagc
     1261 gagttttcct gtgattcgat tcgtcactgg tggtacgaat atggaatcct gtactatcct
     1321 ctggcaacat ccattttaat gttgatggat ggcggcggca gcaactcctc cagacactat
     1381 attttcaaac aggatttaca ggctttagtg gaagagatcg gtattgaaat aaggatagcg
     1441 cattatccgc cgtacacgtc taaatggaat cccattgagc atcgggtatt cccccatatt
     1501 actcgcgaat tatccggcat gattttaacc agccatctat ttatgaaaga gttaatagaa
     1561 aatacaacaa caaaagcagg attaaaagtc tttgcctgta ttttcaacag ggtttatgaa
     1621 actggcagaa aagttgtgga ggggtttaaa gaatcaatgc gaattttatt tgataagcat
     1681 ctggggcaat ggaattatgt tgccattcct gaaacgacaa tttcagaagt tgcctaagtt
     1741 attttatgca cgttcctaag gacaatggac taattgatgg ctttgttttt ttgcattcaa
     1801 aaagcctaaa gcttgtaatt cttgtagaaa aagattttta acaaaaatag cgcgcaatta
     1861 aatttcgtca aagaatagtc aggcgagcta tattacgtca taaataaata actaaacaac
     1921 caccagctaa agctggtggg tttaagttac ggactggaag tcccggatac gcgtcgactt
     1981 aacgacgcgt cttatggagg ctccatctta aattcatcgt tcggattagg ctcaaaatga
     2041 tgctccagat attgtttaat catttcttcc gtcatctgcc caactgtggc gcagaaatat
     2101 ccc
//
LOCUS       DASVIM010000142         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1250783, whole genome shotgun sequence.
ACCESSION   DASVIM010000142 DASVIM010000000
VERSION     DASVIM010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250783"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VF305_02880"
     CDS             complement(<1..783)
                     /locus_tag="VF305_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEX7416110.1"
                     /translation="MSFIQNLLMHIPTPLLGFLMVAIYVALSVTGLIIIRKFYPHYKC
                     KLHNDIAGFIFSTLGVIYAVLLAFTVIITWQDFDKASEVTGNEANCIASLYHDATAFP
                     AGFRAQLKSGLTNYVNAIISDEWQKMAKGQRSPQVQKIQEKLWLLYGSFQPKNETQKI
                     FLMESVEKMNQASEMRRQRIVYAASGIHPLLYFVLITGSFITIAFSMMFGTENLVPHL
                     IMTSFLAVMIALTLFTIIALDYPFTGNVSIKPEVFTNMLSSLM"
     gene            complement(964..1809)
                     /gene="rpoH"
                     /locus_tag="VF305_02885"
     CDS             complement(964..1809)
                     /gene="rpoH"
                     /locus_tag="VF305_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX7416111.1"
                     /translation="MKLPVVVGTLDLYIAEINRFPLLTAEEEFKLAVRLKKHNDMEAA
                     EKLVVSNLRFVVKIAHEYRNYGFKLADLIQEGNIGLIHAVKKFEPYKGYRLISYAVWW
                     IRAYIQNYLIKSWSIVKIGTTQAQRKLFFKLSQAKKQLETLSKKKPEFAEIAESLGVK
                     GSEVAEMDLRMGSRDVSLHEFIGDEGSSSHIDFLAYEGDDQELALIKSEEKSLVRRDI
                     AGALANLSERESYIILNRVMADNPETLQEIGNKFKITRERARQIEKQALKKVQLALPY
                     LKPDK"
BASE COUNT          617 a          454 c          415 g          633 t
ORIGIN      
        1 cattaaagac gacagcatgt tggtgaaaac ctcgggcttg atgctgacgt ttcccgtaaa
       61 cggataatcc agcgcaataa ttgtaaaaag agtaagggca atcattaccg cgagaaatga
      121 tgtcatgatt aaatgcggaa cgaggttttc cgtgccaaac atcatggaaa aggcaatcgt
      181 aataaaactg ccggtgatga gaacaaaata gagaagcgga tgaatgccgg aggcggcata
      241 gacaatgcgc tgcctcctca tttcacttgc ctgattcatt ttttctactg attccattaa
      301 aaaaattttc tgcgtttcat tttttggttg aaaactgccg taaagcagcc acaatttttc
      361 ctgtattttt tgcacttgag gacttctctg tcctttggcc attttctgcc attcgtcgct
      421 gatgatggca tttacataat tagtcagtcc acttttaagc tgggcgcgaa atccggcagg
      481 aaaggccgtg gcatcatgat agagcgacgc tatacaatta gcttcattac ccgtcacttc
      541 acttgcctta tcgaaatcct gccatgtaat gatgaccgta aaggcaagaa ggaccgcgta
      601 aattacacca agagtagaaa aaataaaacc ggcaatatcg ttgtggagtt tgcatttgta
      661 atgtggataa aatttccgga taatgataag gccggttaca gataaagcta cgtagatagc
      721 aaccataaga aaccccaaca acggcgtcgg tatgtgcatg agcaaatttt ggatgaatga
      781 cataattttc tcctcaaaat ttacagctta aatatatatt taagttatta taccgaaaca
      841 tttttcatca acaaaaaggt aagaaatggg gtcagatctt gaaatatcag tttgcaatat
      901 ttcggggaca tcatactagt ttccttttaa aagttaatgc ttacatacta ccattcgtag
      961 cccctattta tccggcttca aatacggcag ggcgagttgc acttttttca gcgcctgctt
     1021 ttcgatttgg cgtgccctct cccgcgttat tttgaacttg ttgcctattt cctgtaaagt
     1081 ttcaggattg tccgccatta cgcgattaag gataatataa ctttctcttt cgctgaggtt
     1141 agccagcgct ccggctatat cacgccggac caaactcttt tcttcacttt taattagtgc
     1201 caattcttga tcatcacctt cgtaagccag aaaatcgata tgcgaactac tgccttcgtc
     1261 accgataaat tcatgcagcg aaacatcccg cgagcccatg cgtaagtcca tttccgccac
     1321 ctcggagcct ttgactccca gagattcggc tatttcggca aactccggct tttttttcga
     1381 aagtgtctcc agttgttttt tcgcctggct caatttgaaa aagagttttc tctgcgcctg
     1441 cgttgtgcct attttaacga tactccagga cttgataaga taattttgaa tgtaggcgcg
     1501 aatccaccat accgcgtaag aaatcagccg gtagcctttg taaggctcaa attttttcac
     1561 ggcgtgaata aggccgatat tcccttcttg aattaaatca gccagcttga agccatagtt
     1621 gcggtattca tgggcaatct tgaccacaaa acgcaaatta gaaactacca gcttttcggc
     1681 agcttccatg tcgttgtgtt ttttcagacg cacagcgagc ttgaattctt cttccgcagt
     1741 caaaagcgga aagcgattaa tttccgcaat atataaatca agagtgccga ctactacagg
     1801 tagtttcaaa gatttgtctc cttttgcatc gatccgctat aactttttat ccaactatga
     1861 ttttccggtc aatatatttt attatgacaa atacttgaca cataaggctt tgatgtttaa
     1921 agtaaataaa acaaattaag gacggttaaa tgaaatcaat aaataagacc atgattgaac
     1981 acgccataaa aattgcgcgc gcggttaaag ctgattcatt tctcgtctgt gttgatatat
     2041 cacctgatat tgccgctctc tcggaggaaa taaagaaaat taccgatgta attattgtta
     2101 cccgcgaaaa tgaagaact
//
LOCUS       DASVIM010000143         6150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1337751, whole genome shotgun sequence.
ACCESSION   DASVIM010000143 DASVIM010000000
VERSION     DASVIM010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337751"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..694
                     /gene="purE"
                     /locus_tag="VF305_02890"
     CDS             170..694
                     /gene="purE"
                     /locus_tag="VF305_02890"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX7416112.1"
                     /translation="MKAKSAKKESILVSVVMGSDSDLPIMTEATKVLEEFDIPHELFL
                     TSAHRTPERTTKFTQSAVGRGVKVIIVGAGAAAHLAGVIAAQTPLPVIGIPIDATSLH
                     GLDALLSTIQMPGGVPVAGMAIGKAGAKNAALFAVRILALEDKKISAKLSAYTGKMAK
                     DVEKKQENISCRKS"
     gene            679..1335
                     /locus_tag="VF305_02895"
     CDS             679..1335
                     /locus_tag="VF305_02895"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX7416113.1"
                     /translation="MPEILKVGADNSEERILTQAAEILANGGIIAYPTETFYGLGADA
                     TNEKAIEKIYTIKGRNSNNPISIIIGYAENLYTLVHNVPLAAQKLMQAFWPGALTIVF
                     QASNKISPLLTAGTGKIGIRLSSHHGTQVIAQRLGRPLTATSANLSGAPECTHASDVA
                     SQIGDKIDAIVDLGPTTDDKGSTIIDVTCDPPVILREGTITRQSIENIFRKKRVLNSN
                     "
     gene            complement(1363..2373)
                     /locus_tag="VF305_02900"
     CDS             complement(1363..2373)
                     /locus_tag="VF305_02900"
                     /inference="COORDINATES: protein motif:HMM:NF025094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX7416114.1"
                     /translation="MIIQCRQCRTKFHFDDALMEEDGVWMRCGSCQHVFFQDNPLIIK
                     PNIDSSLRLEAVFLGESIPAEKSAELSKEGSSVSGRDEDVIRFLDNVMETKNTLNEEV
                     NSKIGTKEGESISEKRHAVPGGSAVIHDLGEKNKKFEQKDTLSKKGFWKTWKVVIWVI
                     LVIIIIPAIIYLIYFKINPQYGDRFTKIVTKIANKYIGTPEPVRPEVVIEQVKLQDIR
                     QRMLNNNVLGQIRVMEGTAVNQADYPISRIVIKGAIVDAYSVVLGERASYAGNILADD
                     ELTNLPEEEILRKLSQPEGLNNSNDKILPNGQIPFMIVFAREPAGVIKTTVTTIGAER
                     LL"
     gene            complement(2390..2914)
                     /gene="hpt"
                     /locus_tag="VF305_02905"
     CDS             complement(2390..2914)
                     /gene="hpt"
                     /locus_tag="VF305_02905"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX7416115.1"
                     /translation="MDEMSKEILFPRAIIQTRVQELASQISGDYAGRELIIIGILKGA
                     FIFMADLIRAMRIPCKVDFVRVASYGAGSESTGKVVMTKDIETSIRGRDILIVEDIVD
                     TGLTLQYLVDWFKERNPQSLKVCAFLDKRKKRKVSFEADYVGFTIDDGFVVGYGLDFS
                     EQYRFLPEIYVIKQ"
     gene            complement(2984..3799)
                     /locus_tag="VF305_02910"
     CDS             complement(2984..3799)
                     /locus_tag="VF305_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416116.1"
                     /translation="MTTDTNFIILCQPDEGKSCGACCGLYNYVDSSRVSLAQRLGSRT
                     KRFRQLVKNREDIEYYAKEMFAGEDFKKRYEVIYCCEYLGFLDSEERKVGCLLHPAQN
                     AGLDLRSVSFYGQELCAGHICPSHHFIPQSQSQILIKIIDDWYLYGLCVTDIDLVTTY
                     FRMLADRIGEELKPEIFDNESLKEIACEFFNWKITWPFRSPGTDRLGKYYFDGSQYMI
                     SYIDYKKFGRDVSPLNTIFLSLSSLFKDAKEIEAAENLLWSNIEKFISTYQCF"
     gene            complement(3949..4110)
                     /locus_tag="VF305_02915"
     CDS             complement(3949..4110)
                     /locus_tag="VF305_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7416117.1"
                     /translation="MKEVNAILLLSCPDRKGLVAKISNFIFQHNGNIVHADQYTSERG
                     QLFFMRVEW"
     gene            4237..4635
                     /locus_tag="VF305_02920"
     CDS             4237..4635
                     /locus_tag="VF305_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416118.1"
                     /translation="MKLIEEINLDNGLKLNIFDLSRPIAADTVKVEIAIQTKIDLKES
                     YFSDRRDYHQVKNIFGDKLAYEHKIERAFVSKDTQDAVRQELVNAFKINSLNYIAAKK
                     FPLKLALAMLKEIKNNPYKYQGRPDSSFEE"
     gene            4670..>6150
                     /locus_tag="VF305_02925"
     CDS             4670..>6150
                     /locus_tag="VF305_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HEX7416119.1"
                     /translation="MTNNFDELLLSSEKPSRYIGAEVNSIRKEKADISFLLAFPDTYE
                     VGMSHLGLQILYSILNEIPYVAAQRCFAVWPDRERQLRLRKLPLTSLESQTPLANFDI
                     IGFSLQYELSYTNVLNMLDLGGIPLARIERKENHPLIIAGGPCCFNPAPLVDFIDAFV
                     IGEGEEVVAEITALIRTCKKQGLPKHDLISRLAQIPGIYVPAIHGKNQIIKKRNPVEL
                     NLWPYPKNPVVPLMQTIHDRIILEIARGCTRGCRFCQAGMLWRPYRERNSSLLLEMAK
                     KLLAATAHDEISLLSLSSGDYSRIEPLIKSLMNQHLSQRVALALPSLRVESLTKTLIE
                     EIRRTRKTSFTLAPEAGTDKMRQIINKGNTAEDLLVTVDKVFAAGWKSIKLYFMLGLP
                     HEEQADLEGIIDLGYQALRAAKNRGQVTISLSTFVPKPHTPFQWERQISLEETYAKQE
                     FIRQRIKNRKLIVKWHDARMGLLEGMFSRGDERLGELLEKAFR"
BASE COUNT         1820 a         1405 c         1227 g         1698 t
ORIGIN      
        1 tctctttcgg gtgaatgata ctctagatat tgcgtgttga tatgtcaagg taaaaactgt
       61 cttgcaatta catttccaat gcgctaattg gacaggcaca attatttatt ccaaagatct
      121 ttttaaggag aaggcgtaag acatttcaaa cattaaaaag gagctgaccg tgaaagccaa
      181 aagcgccaaa aaagaaagta ttctggtaag tgttgttatg ggcagcgatt ccgatttgcc
      241 catcatgacc gaggcgacta aagttctcga agaatttgat atcccgcatg agttgtttct
      301 gacttcagcg caccgcacac cggaacggac tacaaagttt acccaatccg cagtggggcg
      361 cggcgtcaaa gtaatcatcg tcggagcggg agcggcagcg catcttgccg gagtaatagc
      421 cgcgcaaacg cctttgccgg tcatcggcat tcctattgac gctacttctt tacacggtct
      481 cgatgcgctt ctttccacga tacaaatgcc cgggggagtg cctgtagcgg gcatggccat
      541 cggcaaagcc ggcgccaaga atgccgcttt atttgccgtg cgcattcttg ccctggaaga
      601 taaaaaaatc agcgcgaagc tttccgctta tacgggaaag atggccaagg atgtggaaaa
      661 aaagcaggaa aacatttcat gccggaaatc ctgaaagtcg gcgcggataa ttctgaagaa
      721 aggattttaa cccaagctgc agaaatcctg gctaatggtg gaatcattgc ctatccgacg
      781 gaaacttttt atgggttggg cgcggatgca acaaacgaaa aagctatcga aaagatttac
      841 accattaaag gcaggaattc taataatccc atttccataa ttattggtta cgcggaaaat
      901 ctctatacgc tagttcataa tgtgcccctg gccgcccaaa agctgatgca ggcattttgg
      961 ccgggagcgc tgaccatcgt ttttcaggct tcgaataaaa tatcaccgct tctgacagcc
     1021 ggcacgggca agatcggcat acgtctttcc agccatcatg gcacgcaggt aattgcacaa
     1081 aggctcggcc ggcctcttac tgcgacaagc gctaatttat ccggcgcgcc tgaatgcaca
     1141 cacgcatctg atgttgcctc ccaaatcggt gataaaattg atgcgattgt cgatctaggc
     1201 ccaacaaccg acgataaagg ttctacaatt attgatgtta cgtgtgatcc cccggttatc
     1261 ctgcgcgaag gaacaataac caggcaatca attgaaaata tttttaggaa aaaaagggtt
     1321 ttaaacagca attagcacta aatcaagtaa ctaaattgtt ttctaaagaa ggcgctctgc
     1381 gccgattgtt gtcacggttg ttttaataac acctgccggc tcgcgggcaa aaacaatcat
     1441 gaaaggtatt tgtccgttag gcaaaatttt atcgttggaa ttgttgagcc cttccggctg
     1501 ggaaagcttt cttaaaatct cttcctcagg caaattcgta agttcatcat cagccaagat
     1561 atttccggca taactggcac gttcacctaa aactacggag taggcatcaa caatcgcgcc
     1621 cttaattact atccgggaaa tgggataatc ggcttgattt accgctgtgc cttccataac
     1681 gcgaatctga cccagaacat tattattcaa cattcgctgc cgaatgtctt gcaacttgac
     1741 ttgttcgatg acaacttccg gccgtaccgg ttccggcgta ccgatatatt tattagcaat
     1801 tttggtaaca attttggtaa aacggtcacc gtattgtgga ttaattttaa aatagattaa
     1861 atagattata gcgggaatga ttataataac taaaattacc caaataacaa ccttccaagt
     1921 tttccagaaa ccttttttgg ataatgtatc tttttgctca aatttcttat ttttttcgcc
     1981 taaatcatga atcactgcac ttccacccgg aactgcatgt cttttctcgc tgatgctttc
     2041 cccttccttt gttcctattt tcgaatttac ttcttcgttt aaagtatttt ttgtttccat
     2101 gacattatca agaaaacgaa taacatcctc gtcccgtccg gaaacggatg agccttcctt
     2161 cgacaactcc gcactttttt ctgcgggtat ggactctcct aaaaaaacag cctcgagtct
     2221 taacgatgaa tctatgttcg gttttattat cagcggatta tcctgaaaaa aaacgtgctg
     2281 gcaactgccg caacgcatcc acacgccatc ctcttccatc aaagcatcat caaaatgaaa
     2341 ttttgtccgg cattgcctac attgaatgat cattttttct cctcatttgt cactgcttaa
     2401 ttacataaat ctcaggcaaa aagcggtatt gttcactgaa atccagccca taaccaacca
     2461 caaagccatc atcaatggta aagcctacat aatcggcttc aaaggatact tttctttttt
     2521 tacgcttatc caaaaaagcg catactttaa gtgattgggg atttctttcc ttaaaccaat
     2581 caacaagata ttgcaaggtc aggccggtat caactatgtc ttccacaatt aaaatatccc
     2641 ggcctctaat tgaagtttcg atatcttttg tcattactac tttacctgtg ctttcggaac
     2701 ctgctccata acttgctacc ctgacaaaat ctactttaca ggggattctc atcgctcgga
     2761 ttaaatcagc cataaaaatg aatgcgcctt taagaatgcc aatgataatc agctcacggc
     2821 cggcgtaatc gccagatatt tggctggcca actcttgcac ccgtgtttga attatagcgc
     2881 ggggaaaaag gatttctttg gacatttcgt ccatttactg catctcctca aattaatcaa
     2941 tgttctatat tactcaacat aacggctatc tgtcaataaa aaatcaaaaa cactgatatg
     3001 tggaaatgaa cttttcaata tttgaccaaa gcaaattttc cgccgcctca atttccttag
     3061 catctttaaa tagggaagat aagctcaaaa aaattgtatt tagcggcgaa acatctcgtc
     3121 caaatttttt gtagtcaata taactaatca tgtattgcga tccgtcgaag taatatttgc
     3181 ccagccggtc ggttcccggc gaacggaatg gccaggtaat tttccaatta aaaaattcgc
     3241 aggcaatttc ctttaatgac tcgttatcaa atatctccgg tttaagttcc tcaccgattc
     3301 tatcggcgag cattcggaaa tatgttgtga ccaaatcaat atccgtcaca cagaggccat
     3361 aaagatacca gtcatcaatt atttttatca gtatttgaga ttgggactgc ggaataaagt
     3421 gatgactggg gcaaatatgc ccggcacaga gttcctgtcc ataaaacgag acagagcgca
     3481 aatcgaggcc ggcattttgc gccggatgca atagacaacc taccttcctt tcctcgctgt
     3541 ccagaaaacc aaggtattcg caacaataaa ttacctcata tctcttttta aaatcttcgc
     3601 cggcaaacat ttcctttgcg taatattcga tatcttccct gttctttact aactgacgaa
     3661 agcgttttgt gcgcgagcct aaacgctgcg cgagagaaac gcgggaacta tccacataat
     3721 tgtagagacc gcaacaggca ccgcaggatt tgccctcatc cggttggcaa aggatgataa
     3781 aatttgtatc cgttgtcata aacgcctaaa atttttttaa agtgatatta ttaaaacaaa
     3841 gctaattgcg atataattcc aatatgtttg atggcttact caaatacaat agttttcgat
     3901 ccataaacga ggattttgtt ttcccgatgc aggcgcaaag cccttcaatt accattcgac
     3961 cctcataaag aaaagttgtc ctctttctga cgtatactga tcggcgtgaa caatgttgcc
     4021 gttgtgttgg aaaataaaat tagaaatctt cgcgacaagt cccttgcgat cgggacagga
     4081 taaaagtaaa atagcattga cttctttcat ttggtagtct caatttctcc aattttgaat
     4141 ctcgatgtta ttttatagaa ggattggcgt tgcaggtcaa gcgcaaaaat gttaaggaca
     4201 actcccttaa ataaaaaacc ttggaactga acataaatga aattaattga ggaaataaat
     4261 ctggacaatg gacttaagtt aaacattttt gacttatccc gtccaatagc tgcggataca
     4321 gtaaaggtgg aaattgccat tcagactaaa atcgatttaa aagagtctta tttttcagat
     4381 cggcgcgact atcatcaggt aaaaaatata ttcggcgaca agctcgccta cgagcataaa
     4441 atagaaagag cctttgtctc caaagatact caagatgctg ttcgccagga attagttaac
     4501 gcgtttaaaa tcaactctct gaattacatt gccgctaaaa agttccccct aaagctggca
     4561 ctagctatgc tcaaggaaat caaaaacaat ccttacaaat atcaaggtcg tcctgattcg
     4621 tcttttgaag aatagcccac aagctttatt acaaaccgga tagtaaataa tgaccaataa
     4681 tttcgatgaa ctcttactca gctccgaaaa acctagccgc tacatcggcg cggaagtaaa
     4741 ttccatccgc aaagaaaaag ctgacattag ctttctgctt gctttcccgg atacctatga
     4801 agtcggcatg tcgcatctcg gtttgcaaat tttatattcc attttaaacg aaattcccta
     4861 cgtcgcggcc caaagatgct tcgctgtctg gccggataga gaaagacaac tgcgtttgcg
     4921 gaaattgccg ctgacttctc tggaatcaca aacgccgctg gctaatttcg atattattgg
     4981 tttttccctg caatatgaac tttcttacac caacgtactg aacatgctgg atttgggagg
     5041 cataccgctt gcccgcattg agcgtaaaga aaaccatccc ttgataatag ccggtggtcc
     5101 atgctgtttt aatccagcac ctcttgttga ttttattgat gcctttgtca ttggtgaagg
     5161 agaagaagtt gtggcggaaa ttaccgcgtt aatccgcacc tgcaaaaagc aaggcctgcc
     5221 caaacacgat ctgataagcc gactggcaca aatacccggc atttacgtcc ccgctataca
     5281 tggaaaaaat caaatcatta aaaagagaaa tcctgttgaa cttaatctat ggccatatcc
     5341 caagaatcca gttgtcccgc tgatgcaaac cattcatgat cgtataattt tggaaatagc
     5401 ccgcggatgt acccgcggct gccggttttg ccaggccggg atgctctggc gtccttaccg
     5461 cgaaagaaat tcttcattac tcctagaaat ggccaaaaaa ctgctagcgg caaccgctca
     5521 tgacgaaata tcgcttttgt ccttgagttc cggcgattat agccgcatcg aacccttaat
     5581 aaaaagttta atgaaccaac atctttccca acgagtggca ttggctctgc catcgctgcg
     5641 cgtggagtcg ctgaccaaaa cattaattga ggaaataagg cgcacccgca aaaccagctt
     5701 tacactggcg ccggaagcag gaacagataa gatgcgtcag attatcaata agggcaatac
     5761 tgccgaagac ttgcttgtta ctgttgataa agtattcgcc gccggttgga aatcgatcaa
     5821 gctttatttc atgctgggcc tgccccacga agagcaagcc gatttagaag gcattatcga
     5881 cctgggttat caagctctgc gggcagcaaa aaaccgcggt caggttacca tcagcctttc
     5941 caccttcgtt cccaagcctc atactccatt tcaatgggaa agacagatat ctttggaaga
     6001 gacttatgcc aaacaagaat tcattcgcca gcgaatcaaa aaccgcaaac tcatcgttaa
     6061 atggcatgat gcccgaatgg gcttactgga aggaatgttt tcgcggggcg atgaaagatt
     6121 gggagagctt ttggaaaaag cctttcgtaa
//
LOCUS       DASVIM010000144         4568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_313326, whole genome shotgun sequence.
ACCESSION   DASVIM010000144 DASVIM010000000
VERSION     DASVIM010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313326"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VF305_02930"
     CDS             <1..334
                     /locus_tag="VF305_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX7416120.1"
                     /translation="VIFAGGTGNPYFTTDTAAALRAMEIKADIIMKATKVDGVYDSDP
                     IKNKAAIMFKNISYIDVLTKNLKVMDATAISLCRDNLLPIIVFNLQKKGNIRSVICGK
                     KIGTYVGE"
     gene            336..893
                     /gene="frr"
                     /locus_tag="VF305_02935"
     CDS             336..893
                     /gene="frr"
                     /locus_tag="VF305_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX7416121.1"
                     /translation="MKENIFQKLKDEMEKIITSLDKSFGRVRTGRASVSLLDGIKVDY
                     YGVPTPVAQVATLSVPESRLIVIAPWDVSIIGSIEKAIQKSDLGLMPSSDGKVIRLSI
                     PQLTEERRKELVKVVKKMAEEGKIKLRNARRDANEELKDLKKKNNMAEDELFSAQEEV
                     QKLTDRYIEKADKIVAVKEKEIMEI"
     gene            1072..1800
                     /locus_tag="VF305_02940"
     CDS             1072..1800
                     /locus_tag="VF305_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX7416122.1"
                     /translation="MIKIDCNNLPQHIAIIMDGNGRWAKQHTIGRVRGHKKGALAVKT
                     TVRTCREIGIKYLTLFAFSIENWGRPKEEVEALMFLLEEYLTKEISHLQKQGIKLTTI
                     GEIDRLNPSVKEKILQVKESTVKNDKMILNLALSYGGRDDIIYAVKKIIQDNNNGKVN
                     INEINRNNFNRYLYTCGMPDPDLLIRTSGEYRISNFLLWQIAYTELYFTKVLWPDFTK
                     DDLLRAIVSYQKRERRFGLTSEQL"
     gene            1869..2678
                     /locus_tag="VF305_02945"
     CDS             1869..2678
                     /locus_tag="VF305_02945"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX7416123.1"
                     /translation="MINSYLKRWLTGIVLAVVIVVVILFCSAEVLAVVITIFIFGGVW
                     EYNNIVFRKGFFKEKIEGLIFAIVIPLVMFFGNNQLLLAVLAFCILFVFILFLWSVKE
                     STFDILSVAKVIFGMMYIPFLMSHLILLRLLENGIIWVLFVLVLAFAGDITALYVGKY
                     FGKHKLIPLVSPGKTVEGTIGLVLGSTMACLIFGYFLFPKVSLIHIAILAFVGSIIGQ
                     LGDLCESAIKRNYGLKDASSLLPGHGGLLDRMDCLIFITPVVYYYLMYVIG"
     gene            2679..3842
                     /locus_tag="VF305_02950"
     CDS             2679..3842
                     /locus_tag="VF305_02950"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX7416124.1"
                     /translation="MKKITILGSTGSIGSSALDVIEKNPERFQVVALAAGQNVSLLKK
                     QIEKFRPKLVAVSTKKNALKLREALTTKNKVKILYDEKGLQEIASLPAADIIISAISG
                     AAGLMPTLAAIEAGKDVALANKETMVIAGEIVTKRAKKKGVKIIPVDSEHSAIFQCLQ
                     GQPHKNLRRIILTASGGPFLNFTKNELRKVKLHQALKHPRWKMGKKITIDSASLMNKG
                     LEVIEAKWFFNIDISNIDVIVHPQSIVHSLVEFIDGTFLAQMGIPDMKIPIAYALTYP
                     ERIINDLQPLNLVKIVNLEFRQPDIKKFPCLGLAYAAGICGGTTPAVLNAANEIAVSA
                     FIEKKIDFIDLPKIIEKVLVLHNSMKDPSLEEILQADLWARRETKKIIERMTS"
     gene            3839..>4568
                     /gene="rseP"
                     /locus_tag="VF305_02955"
     CDS             3839..>4568
                     /gene="rseP"
                     /locus_tag="VF305_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX7416125.1"
                     /translation="MITIISFVILLGVLIFVHELGHFLAARIAGVGVLKFSLGFGPKI
                     IGKKIGETEYALSWIPLGGFVKLLGESGNEELPPEDEKRSFLKQPTWKRMLIILAGPV
                     FNFLLALVIFIIVFIYGLPNLTANVGEVQKESAAFEAGMMSGDKIITIDGKKVVFWEE
                     IKPIIAEGKGKQVEVTVERGIVKKNILIKPKLSKSKNIFGEEISAYLMGISPAGNMVV
                     ERKNPWEAIIAAVNKTWEISKLTII"
BASE COUNT         1484 a          727 c          991 g         1366 t
ORIGIN      
        1 agtgatattt gccggaggaa ctggcaatcc ttattttacg acggatacag ctgcagcttt
       61 aagggcaatg gaaataaaag cggatattat catgaaggcc acaaaagttg acggagtata
      121 tgatagcgat cccataaaga acaaagcggc tattatgttt aaaaatataa gttatattga
      181 tgtattgacg aaaaacctta aagtaatgga tgcaaccgca atatctcttt gtcgtgacaa
      241 tttgttgcca attattgttt ttaatttaca gaagaaggga aatattcgca gtgtaatatg
      301 cggcaaaaaa attggtactt acgtgggaga atgatatgaa agaaaatatt tttcagaaac
      361 ttaaagatga aatggaaaag attattacaa gtttagataa gtctttcggc agggtaagaa
      421 cgggaagagc atccgtatcg ctcttggatg gaattaaagt tgattattac ggtgttccta
      481 cgccagttgc ccaggtggcc acactttccg tgccggaaag ccgtttaatt gttatagctc
      541 cctgggatgt atctataatc ggctcaatcg agaaagctat tcaaaaatcg gatttaggtt
      601 taatgccgtc aagcgacggc aaagtaatac gtctctctat tccacaatta acagaggaga
      661 ggcgcaaaga gctggttaaa gttgtgaaga aaatggcaga agaaggaaaa atcaagttac
      721 gtaatgcacg cagggatgct aatgaagaat taaaagattt aaagaaaaaa aataatatgg
      781 ctgaagacga acttttcagc gctcaggaag aagttcagaa attaactgac cgctacatcg
      841 aaaaagcgga taagatcgtc gctgtaaaag aaaaagaaat aatggagatt taaactaaat
      901 tttagataaa tagctaaatg gggagtccaa agctccccat ttgtttttct gccaaaactt
      961 cgttcggaga tgaacttttc gtattatttc ttgatatatt tgaatcagat cgagtgttga
     1021 aaagtgttac cctgtctgtt agagtattaa aataatcagg agctggatat agtgataaaa
     1081 attgattgca ataatttgcc gcagcacata gccataatca tggatggtaa tggacgttgg
     1141 gctaaacagc atacaattgg tcgagtccga ggtcataaaa aaggagctct ggcagtaaag
     1201 acaactgtcc gcacctgccg tgaaatcggt ataaaatatc ttaccctatt tgccttttcc
     1261 atagaaaatt ggggacgtcc gaaagaagaa gtggaagctc taatgttctt gctggaagag
     1321 tatttaacca aagaaataag tcatttacag aaacaaggaa taaaactcac aaccattgga
     1381 gaaatagacc gtttaaatcc gtcagtaaaa gaaaaaatct tgcaggtaaa agaaagcaca
     1441 gtaaaaaatg acaagatgat tttgaatttg gccttgagtt acggaggtcg agacgacatt
     1501 atctatgctg taaaaaaaat tattcaagat aataataatg gtaaagtaaa tataaatgag
     1561 attaatagga ataattttaa tcgttattta tatacgtgtg gaatgcccga tcccgatctc
     1621 ttaatccgca ccagtggcga gtatcgcatc agtaattttt tactctggca gatagcttat
     1681 acggaacttt attttacaaa agtcctttgg cctgacttta caaaagatga tcttttaaga
     1741 gcaattgtaa gctatcaaaa acgcgaaagg cgctttggtc tgaccagtga acaattatag
     1801 cgggaaatct tgtggttgat tattaaaatg agcttactta aaattacagg tatgaataaa
     1861 ggttagaaat gataaattcc tatctaaaac gatggttgac aggtatagtt ttagctgtcg
     1921 taatagttgt ggttatttta ttttgttcag cggaagtttt ggctgtcgtt attaccatat
     1981 ttatttttgg tggagtttgg gaatacaata atatcgtttt cagaaaggga tttttcaagg
     2041 aaaaaattga aggtctgatt tttgcaattg tgatcccctt agttatgttc tttggaaata
     2101 atcagctttt gcttgccgta ttggcctttt gtattttgtt tgtatttatt ttatttcttt
     2161 ggtcggttaa agaatcaact tttgatattc tatcagtggc caaggttatt tttggcatga
     2221 tgtatatacc gtttttaatg tctcatctta ttttgctgcg actgttggaa aatggaatta
     2281 tctgggtgtt gtttgttctg gtactcgctt ttgcgggaga cataactgct ttatatgtag
     2341 gtaaatattt cggtaaacat aaattaattc ccttggtcag ccccggtaaa actgttgaag
     2401 gcacgatagg attggtttta ggaagcacta tggcttgctt gatcttcggt tattttttat
     2461 tccccaaagt atcattaatt catatagcca ttcttgcttt tgtgggcagt ataatcggac
     2521 aactgggaga tctttgcgaa tcggcaatca agagaaatta tgggctcaag gatgcaagtt
     2581 cacttttacc cggccatggc ggcctgcttg atcgtatgga ttgcctgatt ttcataaccc
     2641 ccgtcgttta ttattacctt atgtatgtaa ttggctaaat gaagaaaata acgatattag
     2701 gctcaacagg ttcaatcgga tctagcgcgc tggatgttat cgaaaaaaat ccggagcgct
     2761 ttcaggtagt ggcgcttgcc gccggtcaaa atgtaagttt actcaaaaag caaattgaaa
     2821 aattcaggcc aaagctggtt gctgtaagca ctaaaaagaa cgctcttaaa ctgcgcgagg
     2881 ccttaacaac taaaaataaa gttaaaatac tttacgacga aaaaggctta caagaaattg
     2941 cttctttgcc agcggcagac attatcattt ctgctatatc gggagctgct ggtttaatgc
     3001 caacactggc agcaattgaa gcgggtaagg atgtagcttt agccaacaaa gagacaatgg
     3061 tgatagccgg ggaaattgta accaaaagag ctaagaagaa aggtgttaaa attatccccg
     3121 tggatagcga gcacagcgca atatttcaat gcttacaagg acagccgcac aaaaatttac
     3181 ggcggataat attaactgct tcgggtggtc cgttcttgaa ttttactaaa aatgaattga
     3241 gaaaagtaaa actccatcaa gcgctaaaac atccgcgttg gaagatgggc aaaaagataa
     3301 ctattgattc tgcttccctg atgaataaag ggctggaagt gatcgaagct aaatggtttt
     3361 ttaatataga tatcagcaat attgatgtta tagttcatcc gcaaagcata gtgcattctc
     3421 tggtcgagtt tattgacggc acttttttgg cgcaaatggg cattcccgat atgaaaatac
     3481 ctattgctta tgctttaact tatccggaaa ggataattaa tgatcttcaa cctctaaatc
     3541 ttgtgaaaat agttaattta gaatttcgcc agccggatat aaagaagttt ccctgtctgg
     3601 gacttgctta tgcggccggt atttgcggtg gcacgactcc ggcggttttg aatgccgcca
     3661 atgaaatcgc cgtatctgca ttcattgaga agaaaattga ttttattgat ttgcctaaaa
     3721 tcatcgagaa agttcttgtt ctccataatt caatgaagga tccctcctta gaagaaattc
     3781 tacaagcaga tttgtgggcg agaagagaaa ctaaaaagat aatagaaagg atgacatctt
     3841 gattactata atttcctttg taattttact tggtgttctt atttttgtcc atgaactggg
     3901 gcactttctg gctgcgcgaa ttgccggtgt gggcgttctt aaattttctc tcggttttgg
     3961 gccaaagatc attggtaaaa aaattggaga gacggaatat gcactttcct ggattccttt
     4021 aggcggattt gtcaaactcc tgggtgagtc gggtaatgaa gaattaccac cggaagatga
     4081 aaaaaggtca tttttgaaac agcccacatg gaaacgcatg ctaattattc tagccggtcc
     4141 cgtttttaac tttttattag cgctggtaat atttatcatt gttttcatat atggattgcc
     4201 caatctaact gctaacgtgg gtgaagtaca aaaagagtct gcagcttttg aagcaggtat
     4261 gatgagtggc gataagatca ttaccattga tggcaaaaaa gttgtctttt gggaggagat
     4321 aaaaccaata atagctgaag gtaaaggcaa acaagtggaa gttactgtgg agaggggtat
     4381 agtaaagaaa aatattttaa ttaaaccaaa attatccaaa tcaaagaata tttttggcga
     4441 agaaatatct gcgtacctca tgggaatatc tcctgccgga aatatggtag tggaaagaaa
     4501 aaatccctgg gaagcaatca ttgccgcagt aaacaaaact tgggaaatta gcaaactgac
     4561 gattattt
//
LOCUS       DASVIM010000145         8035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_911371, whole genome shotgun sequence.
ACCESSION   DASVIM010000145 DASVIM010000000
VERSION     DASVIM010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911371"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VF305_02960"
     CDS             <1..1031
                     /locus_tag="VF305_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7416126.1"
                     /translation="MTINLQGTIKTFNRAAEEITGFPLQSIENRQIEEFFPEFMPFFS
                     AQKVNIQSKSRIETIIKGEKGIKIHLGCAISPLKDKNEKQIGNILIFQDITEIRLMEE
                     NLEKSKRLALIGEMAAGLAHEMRNPLASITGSIELLKQGLNLEETDKRLMQIILRGKN
                     QLDSFVRDFLLLARPIPLSRELVDINMVAEEVLENIKLSSEWSSKIKIMKVFSDNAAI
                     FANRGQIRQIIHNLVLNAVQAMEKGGVLSVETALVKLDDKKEYAEIKITDTGCGIDQG
                     NLKKIFEPFFTNKDKGTGLGLTIVGRIVEAYSGKIKIESNINIGTSCAVWLPAKDENI
                     QQKNLYNA"
     gene            1051..2415
                     /locus_tag="VF305_02965"
     CDS             1051..2415
                     /locus_tag="VF305_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7416127.1"
                     /translation="MARILVVDDDQGMREFMEIMLTKEGYSVTCAEDPSKAITLCNKK
                     DFDLVITDLRMPKIDGIEFLKTVKDKKPETIVILITAYASGETAVNAMKEGAYDYVEK
                     GGSIEELKKIVRAALVKKGLLTENEQLKKEQVEDIRFGGMIGTSREMVKVFATIKKVA
                     DTPANILILGESGTGKELVARAIHENSPRSKMPFNAINSGGIPENLLESELFGYMKGS
                     FTGAYADKPGLFEMARGGTIFLDEIGELPPVLQVKLLRVVQEKTFRRIGGAEDIKVDV
                     RIISATHQNLEEKVKKGEFREDLYFRLNVIPLHMPPLRERKEDIPLLTKHFIEKYAKE
                     FGKEIRTISSYAMELLMNYAFPGNIRELENIIERGVAMESSNIILPENLVLAANAASG
                     VQNFDVGITDAGIDLNAELEKMEKAMIEKALQKTKGSKTKAAELLHVTFDSLRYRMEK
                     LGVE"
     gene            complement(2501..3775)
                     /locus_tag="VF305_02970"
     CDS             complement(2501..3775)
                     /locus_tag="VF305_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7416128.1"
                     /translation="MNQRFTDLILAAIKQRLSDLHITGGFPLIFRSDGVIHFDKNIKW
                     SHTEIDAMVKEMLSDRQLQMLRKRWSVDFALSFNYVRVRINAFYTTRGLSLAIRLLPE
                     TIPDLTSLNLHPSLSQILELQSGLILVCGSTGSGKSTTIAAIIEEINRTRPAHIITIE
                     DPIEYRFKPKKSFIEQREVGIHVPSFDQGLLDVLRENPDVIFVGELREPDTIRLTLNA
                     AESGHLVFATLHASDAEDAAYRINNCVTGENRDVIRYQFASTLSWLIVQQLTLIKKVG
                     FRVPMLSILRATPSVQSIIRENKLTQLENIMSTSKNDGMFTMKLYEKEFINNRSSFVP
                     PYKSFGMGADSSKEQDYISSLIDPNAVQDVIYMSSVDDAYKTDGASKLTVGDEDEQRY
                     VILEDHDMDLKDLLAQYDESKEEKIEKKRPKK"
     gene            4283..5488
                     /locus_tag="VF305_02975"
     CDS             4283..5488
                     /locus_tag="VF305_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7416129.1"
                     /translation="MGKKINILIAMMAVVFLFTGIVSVQAKTVKVGVAINLTGPASTW
                     GQFHEKGQRDYFRYVNEVKGGVFGNKIDMITVDTAYKVPEAIAAVQKFALQDKVDMIA
                     TWGAGEGLAAKPIVQKYKIPTINYSTSWEILEKPIGYMYLPFGSYKMDCYAVLEYIKT
                     IHKGKDAPKVGLLTYNNAYGRSIHEPSKEYAAKLNINIVAIEEFPPKTVDLNTELLRL
                     QKAGAEYIFMQMLPSAIITAFKSADRINFSPLFLGTWTSTDPDFFKMSSGLIRDRLVI
                     QFPGTLPTDKSAGMKIMKELWKRYKTVDKFDASYWEGVAIGMIMERAFIRAYEKSKDI
                     NPETINAAMESFKDEDFGGLVPKITYSAEDHGASFTARMVKVEEDGTYKPLTNFYIPG
                     KDKIKLLKK"
     gene            5532..6320
                     /locus_tag="VF305_02980"
     CDS             5532..6320
                     /locus_tag="VF305_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7416130.1"
                     /translation="MSSRREEDKKAELEIKNLHLNFGGVTAVKDVDLNVYTGELMAII
                     GPNGAGKNSLLNCITGFYRPQKGKIVFNNKDITNLHTHELVGVGIGRTFQNIELFPGM
                     TVLANMTLARHIHCNYSFIQSCLFSPAVKREEIRHRQILEEIIDFLEMQSIRKKNVGS
                     LPYGMMKRVELGRALALEPRLLILDEPFAGMNLEEKEDMVRFLLELNQVWGLTIILVE
                     HDMSIIMSISNRIMVLNFGETIGEGKPEEISQHPEVIKAYLGDA"
     gene            6313..>8035
                     /locus_tag="VF305_02985"
     CDS             6313..>8035
                     /locus_tag="VF305_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7416131.1"
                     /translation="MLKQYIKKREKGVNNLDSATRKKVYDYNFTLPQILAENAAKYSN
                     EKIAIREKAYGIWQKYSWSDYYCYMRKTAGGFAALNLQRGENVCLIVGNQPEWLFCEI
                     SAHALRATTLNLFTSSVAEELSFSITRVNSPIVVVQDQEQVDKLLEVKEKLSQIKKVI
                     YIDPTGMTSYENNPWLISYAQLLEIGEKFNSENQHFIDEEISKGRPEDIAVMIQTSGT
                     TGIPKLAMLSHRNIIQMSLQWVEATGIKPEENWLSMSPPAWIVDQMWGMGVALISAMT
                     MNFPETAETVLEDFRDIGPSIIITSSRFWEDMASRIRVKISDAGWLKKKLFYWGETVG
                     KAVVEKQAKNISISLPLNILYKLLSFLVYRPLLDRMGCALFRSAFTGGHPISPDVILF
                     FRAIGLNLKQCYGLTEACGIFQIQPDNEVKLETVGKPLPQTEVKIAADQEVLVRSLAN
                     FSGYYNDYESTQAAYDDGWLKTGDAGYIDGDGHLLIIGRKEDIIRNKSGEAFSPDFIE
                     TRLKFSPYIKEAVIFGEGRPFISAMINIDMGNAGNWAEERMIPYTTYMDLSQQSAVEE
                     LILKEVRG"
BASE COUNT         2505 a         1460 c         1837 g         2233 t
ORIGIN      
        1 tcatgaccat taatttacag ggcactatta agacttttaa tcgagcagcg gaagagatta
       61 ccggattccc cctgcagagc atagaaaaca gacaaattga ggaatttttc ccggaattta
      121 tgccattttt ttcagcgcag aaggttaata tacagagcaa aagccgaata gaaactatta
      181 tcaagggaga aaaggggata aaaattcatc tgggctgtgc gatttccccc ttaaaggaca
      241 aaaacgaaaa acaaataggg aatattctga tttttcagga cattacagaa attaggctga
      301 tggaagagaa cctcgaaaag agtaagaggt tggctttaat cggagaaatg gccgcaggat
      361 tggcgcatga aatgagaaat ccgctggctt cgataaccgg ttcaattgag cttttgaaac
      421 agggactgaa cttagaagaa accgataagc gcctaatgca gataatttta cgcggcaaaa
      481 atcaactgga tagttttgta cgggactttc ttctgctggc gcgtcccatt cctttgtcca
      541 gggagctggt tgatataaac atggttgccg aagaagttct cgaaaacata aaattatcca
      601 gtgaatggtc gagtaaaata aaaattatga aagtattttc agataatgcc gctatttttg
      661 ccaatagggg acagatcaga caaattattc acaatcttgt tcttaatgcc gttcaggcaa
      721 tggaaaaagg aggcgtttta tctgtggaaa ctgcattagt aaaattagac gataaaaagg
      781 aatacgctga aattaaaatt acggataccg gctgcggcat tgatcaagga aatttgaaaa
      841 aaatatttga gccttttttt accaataagg ataagggcac cggtttaggt ttaacgatag
      901 tcggccgtat tgttgaagcc tacagtggaa aaataaagat agagagcaat ataaatattg
      961 gtacaagttg cgccgtttgg ctgcctgcaa aagatgaaaa tattcagcaa aaaaatttat
     1021 ataatgcgta agtaaaactg aggtgaggct atggccagaa ttttagttgt tgacgacgat
     1081 cagggcatga gagaatttat ggagataatg ttaacgaaag aaggctatag tgtaacttgc
     1141 gccgaagatc cttccaaagc aatcactctc tgcaataaaa aagactttga tttggttatc
     1201 actgatttga gaatgcctaa aattgacggc attgagtttc ttaaaaccgt taaggataaa
     1261 aaaccggaaa caatagtaat acttattact gcttatgctt cgggggaaac agccgtaaac
     1321 gccatgaaag aaggcgctta tgattatgtg gaaaaaggag gcagtattga agaactgaaa
     1381 aaaatagtgc gcgccgcttt ggtaaaaaaa ggattattaa ccgaaaatga gcagttaaaa
     1441 aaagagcaag tcgaggatat aaggtttggc gggatgattg gaacaagccg ggaaatggtg
     1501 aaagtattcg cgacaattaa aaaagttgcg gacactccgg caaatatttt gatcttagga
     1561 gaaagcggaa caggtaaaga actggtcgcg cgcgcaattc atgaaaactc accgcgttcc
     1621 aaaatgcctt ttaatgccat taacagtggc ggaataccgg aaaatttgct ggagagcgaa
     1681 cttttcggct atatgaaagg ttcgtttact ggcgcttatg ccgataagcc gggacttttt
     1741 gagatggcgc gaggaggcac tatatttctc gatgaaatcg gggaattacc tcctgtactt
     1801 caagtaaaac ttttacgtgt agttcaggaa aaaacctttc ggcgcatcgg cggagctgag
     1861 gatatcaagg ttgatgtacg gataatttcc gctactcatc aaaatttgga agagaaagtc
     1921 aaaaaagggg agtttaggga ggatctttat tttcggctga atgtcattcc tcttcatatg
     1981 ccgccgctga gagaaagaaa agaagacata cctcttctaa cgaagcattt tattgagaaa
     2041 tacgcgaaag aatttggcaa ggaaataaga acaatttctt cttatgcgat ggagttgctg
     2101 atgaattatg cctttcccgg caatatccgt gaattggaaa atatcataga acgcggcgta
     2161 gccatggaga gctccaatat aatcttgcca gaaaatttag tattggccgc taatgccgcc
     2221 tctggcgtgc aaaattttga tgtcgggatt acagatgcgg gcatagactt aaatgcggag
     2281 ttggaaaaaa tggaaaaagc aatgatcgaa aaagctctgc aaaaaactaa aggctccaaa
     2341 acaaaagcag ccgaactatt gcatgtaacc tttgattcgc tacgataccg tatggaaaaa
     2401 ttaggcgtgg aatagatcag tttgcattat gcgggatatt tataggaagc tgtccaaaaa
     2461 gcgtttcgag cagactccac tggttacgca gggtgttagg ctattttttg gggcgctttt
     2521 tttctatttt ctcttctttt gattcgtcat actgggcgag aaggtctttg agatccatgt
     2581 catggtcttc caggattaca tagcgttgct catcttcgtc accaacggtt aattttgagg
     2641 cgccgtcggt tttgtaggcg tcatctacag aggacatata aattacatcc tgaacggcgt
     2701 tgggatcgat aagagaggat atataatctt gttctttaga tgaatcagct cccatgccga
     2761 aactcttgta aggagggaca aacgaggagc gattgttgat gaattctttt tcatatagct
     2821 tcatcgtaaa cataccgtcg tttttgctgg ttgacatgat attttccaat tgagtcaatt
     2881 tattttcgcg aatgatgctc tgcacggaag gcgttgccct aagtatggac agcatgggaa
     2941 ctcgaaaacc aacttttttg ataagggtca gttgttgaac aataagccag gaaagcgttg
     3001 aggcaaactg atagcggata acatcccggt tctcgccggt aacgcagtta ttgatgcgat
     3061 aagccgcatc ttcagcgtct gaggcgtgaa gtgtcgcgaa gacaaggtgt ccggactcgg
     3121 cggcattaag agtaaggcga atggtatccg gttcccttaa ttcccccaca aaaataacat
     3181 caggattttc tcttaataca tccagaagcc cctgatcaaa tgacggtaca tgaatgccta
     3241 cctcgcgctg ttcaataaaa gattttttag gtttaaaacg atattcaata ggatcttcga
     3301 tggttatgat atgcgccggc ctggtgcgat ttatctcctc aattatcgct gcgatggtgg
     3361 ttgatttacc cgagccggtg ctcccacaaa ccagaatcag ccctgattga agctccagaa
     3421 tttggctgag cgagggatgc aggttgagcg aggtgaggtc gggaatcgtt tccggaagca
     3481 ggcgtatagc aagactcaac ccacgggttg tataaaacgc attgattcta acacgcacat
     3541 agttgaaaga aagggcgaaa tcaacagacc atctttttct gagcatttgc agttgcctat
     3601 cgctcagcat ttccttgacc atagcgtcta tttccgtatg cgaccatttg atatttttat
     3661 cgaaatggat gacgccgtcg ctacggaaaa tcaagggaaa tcctccagtg atgtggaggt
     3721 cggaaagcct ttgtttgatg gcggcaagga tcaaatctgt aaaacgctga ttcataaatt
     3781 attcccagat agtactattg ccatcattaa tttaaattat gatacttctg tttgataaaa
     3841 actttctttt attagctata tataataaaa aagttgtctg cgtgtcaaga taattaaact
     3901 atatgatatc gtagttttaa tgttaatatt tattagtata acatggattt ttaattagcc
     3961 ataaacttgc caggtattaa taagtttata atattttaat gattgtcgaa aatgattatt
     4021 tacttttttc ttgacttttt ccgcatattt actctatatg cccatgcaag gcaaggcgta
     4081 aaatgtgtat gattaaaaaa ttgttttcta agcaggcaac ttttccccct ttttttatct
     4141 ttaccgtcat aaaggtctgc ctgaaatgat catgacccat tatcaaggga aatgaaccgt
     4201 gggcagatca atatcagccc acggtttttt ttgttttgaa ggttagaaaa ataaatattt
     4261 ggagttagca agggagggaa aaatgggaaa aaagattaat attttaatag caatgatggc
     4321 tgttgttttc ttgtttaccg gtattgtcag tgtgcaagcc aagacagtaa aagtgggcgt
     4381 tgcgatcaac ttgactgggc cggcttccac ttgggggcag tttcatgaaa aaggccagcg
     4441 agattatttc cgctacgtaa atgaagttaa gggcggagta tttggcaaca aaatcgatat
     4501 gatcactgta gacacggctt acaaagttcc ggaggctatc gctgccgtgc aaaaatttgc
     4561 ccttcaggat aaggtggata tgatcgctac atggggcgct ggtgaaggtt tggccgctaa
     4621 accgattgtt caaaaatata aaataccgac tattaattat tccactagct gggaaatcct
     4681 cgaaaagccg attggctata tgtatcttcc tttcggcagc tataaaatgg attgttatgc
     4741 ggtgttggaa tatattaaaa ctatccacaa aggaaaagac gcgccgaaag taggcctttt
     4801 aacgtacaat aacgcttatg gacgctcaat tcacgaaccc agcaaagagt acgccgcgaa
     4861 acttaacatt aatattgttg ccatcgagga atttccgccg aagaccgttg atttaaacac
     4921 cgaattgctt cgtctgcaaa aagcaggtgc ggaatatatc tttatgcaaa tgttgccttc
     4981 ggcaataata actgccttca agagtgccga ccgcattaat tttagccctc tgtttttagg
     5041 aacatggaca tccactgacc cggatttctt caaaatgagc agcgggctga ttcgtgatag
     5101 gcttgttatt caatttcccg gaacattgcc gacggacaaa tccgctggaa tgaaaataat
     5161 gaaggagtta tggaagcgtt ataaaacagt ggataagttc gatgcttctt attgggaagg
     5221 cgttgccatc ggcatgatta tggagagagc ctttatccgt gcttatgaaa agagcaagga
     5281 tattaatccg gaaacaatca acgccgctat ggaatcattt aaagatgaag attttggcgg
     5341 cttggtgccc aaaataacat atagcgctga agatcatggt gcatcattta ctgcccgcat
     5401 ggtcaaagtt gaagaagatg gaacatataa gccgttgact aatttttata taccgggcaa
     5461 ggacaaaatt aaattgctca aaaagtaatt gaaagatttt catgaaagct tttcgcggcc
     5521 gaccatcgat aatgagcagt cggcgagaag aagataaaaa ggccgagtta gaaattaaaa
     5581 atctgcatct taattttggc ggcgttacag ccgtgaagga tgttgatctt aacgtttata
     5641 ccggcgaatt gatggctatt atcggtccca atggggcggg taaaaacagt ttgcttaact
     5701 gcattacggg gttttaccgc ccccaaaagg gaaagatagt atttaacaac aaggacatta
     5761 ctaatctcca tactcacgag cttgttggag tgggtatagg cagaacattt caaaatattg
     5821 aactatttcc aggaatgacg gtgctcgcca atatgacgct tgcccgccat attcactgta
     5881 attacagctt tattcaatct tgtctttttt ctccggcggt caagagagaa gaaatccgtc
     5941 accggcaaat tttggaagaa ataattgatt ttctggaaat gcaatcaatc cgcaaaaaaa
     6001 atgtgggttc cctgccttat ggaatgatga aaagagttga gttgggaaga gctttggcgc
     6061 tggagccgcg cttgcttatt ttagacgagc cttttgccgg catgaacctg gaagaaaaag
     6121 aagatatggt tcgttttctg ctggaattaa accaagtatg ggggctcacg ataattttag
     6181 ttgagcatga tatgtctatc atcatgagca tttctaatcg tatcatggtc ttgaattttg
     6241 gtgaaactat cggcgaggga aaacctgaag aaataagtca acatccggaa gttatcaaag
     6301 catatcttgg tgatgcttaa gcaatatata aaaaaacggg aaaaaggcgt aaacaatttg
     6361 gatagcgcaa cacgtaaaaa agtttacgac tataatttta cattgccgca aatcctggct
     6421 gaaaatgctg ccaaatatag caatgaaaaa atagcaattc gggaaaaagc atacggcatt
     6481 tggcagaaat atagctggag tgattactat tgctatatgc gaaaaacagc cggtggtttt
     6541 gctgctttga atcttcaacg cggtgaaaat gtttgtctga ttgttggaaa tcaaccggaa
     6601 tggctttttt gtgaaatttc ggcgcatgct cttagagcta ccacactcaa tttattcact
     6661 tcatcagttg ctgaagaatt gtctttttct attacacgcg tcaattctcc catcgtagta
     6721 gttcaggatc aggaacaagt tgataagctc ttagaagtga aagagaagtt atcgcaaatc
     6781 aagaaagtta tatatattga tcccacggga atgacatctt atgaaaataa tccgtggctg
     6841 attagctatg ctcagctttt agaaataggc gaaaaattta attcggaaaa tcagcacttt
     6901 atcgatgaag aaataagtaa aggccgccct gaagatattg cagttatgat tcaaacttcg
     6961 ggaactaccg gcattccaaa attggccatg ctttcccatc gtaatataat acagatgtct
     7021 ctccagtggg tagaagcaac gggaattaag ccggaagaaa actggctatc catgtcgccg
     7081 ccggcctgga ttgtcgatca aatgtggggt atgggtgttg ccttaataag cgcaatgacg
     7141 atgaattttc cggaaaccgc agaaacggta ttggaagatt ttcgcgatat cggtccttct
     7201 ataataatta catcgtcacg cttttgggaa gatatggctt cacgtattag agtcaaaata
     7261 tctgacgccg gttggttgaa gaaaaagtta ttttattggg gggagaccgt gggcaaagcg
     7321 gttgttgaaa aacaggcaaa aaatatttcc atttctttgc cgttaaacat tttatataaa
     7381 cttttgtcat tcttagttta ccggcccctt ttagatcgta tgggctgcgc tctttttcgt
     7441 agtgctttta ccggcggaca ccccataagt cccgatgtaa ttcttttctt ccgcgccatt
     7501 ggcttgaatt taaaacaatg ctatggcctc accgaagcat gtggcatatt tcaaatccag
     7561 ccggacaacg aagtaaaact cgaaactgtg ggaaaacctt tgccgcaaac cgaggtaaaa
     7621 atcgctgccg atcaggaagt attggtaaga tctcttgcta atttttccgg ctattataat
     7681 gattatgagt caacacaagc cgcctatgac gacggatggc tgaaaaccgg cgatgccgga
     7741 tatattgacg gcgacggcca tttattgatt ataggccgca aagaagatat tatccgcaac
     7801 aagagcggcg aggctttttc acctgatttt atcgaaaccc gcttgaagtt cagcccctat
     7861 ataaaggaag ctgtaatttt tggcgaaggc cgtccattta tcagcgccat gatcaacatc
     7921 gatatgggca atgccggcaa ctgggcggag gaacgcatga ttccttacac aacttatatg
     7981 gatctttcac aacaatcggc cgtggaagaa ttaatattaa aagaagtgag aggga
//
LOCUS       DASVIM010000146         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_143462, whole genome shotgun sequence.
ACCESSION   DASVIM010000146 DASVIM010000000
VERSION     DASVIM010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143462"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(369..1688)
                     /locus_tag="VF305_02990"
     CDS             complement(369..1688)
                     /locus_tag="VF305_02990"
                     /inference="COORDINATES: protein motif:HMM:NF033539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX7416132.1"
                     /translation="MEFDISYTSKEITPWGGMVFLKQMLQKTGFRELIDQNTDLPQSG
                     SNRGYKTSTIIEGFITSIWCGANRFLHTEVTRHDSALGKIFDWKNTPGQDTYKRFFSK
                     FTQAKNHNISQYFYSWIFDNFKFDNFTLDIDSSVMTRYGVQQGAKKGYNPAKRGRPSH
                     HPLIAFIADVKLVANMWLRSGDTSSANNFLGFLEDTLSKLKNKTVSLIRLDSGFFQSN
                     ILDYLEFKTMDYVVAAKFSQPIQRLIAASNNWILLDTGIEICEQLYQSDSWTKSRRLV
                     IVRQTLKDRPKATGKQLGLFAEQEIYKNYRYSAYLTNLKLAPAEIWRLYRGRGDAENR
                     IKELKYDFGFDSFNLKDFYATEAALTFAMIAYNLMALFRTFVLQEKTQKTLSTLRYRT
                     FAIGAYFEKLNDKLVLKIALSKKRRAWFSGLWNYAKVFEYPFEFSIA"
BASE COUNT          653 a          387 c          366 g          713 t
ORIGIN      
        1 aaccactaca gtcttcaata atatcctgcc agccttaata cctgctgcat taactttcat
       61 gaaccgtatt attgctatta ctcgcaggaa tgctttatta attatcaaaa gttttttcta
      121 atcgctacca tttgaaaagt aataaaaatg cctttattct cctttaacca atcatatccg
      181 tgggaatttt aattacccat gtgattccca tcaaacgtag ttgagcgagg tatgatttgt
      241 aaaatatttt taggaagaca attgagataa aacgctacca aaagataagg atgataggaa
      301 gttttggtat gctgctgcat tggagtccgg atgataattt atataaagcc acttccattt
      361 aacccaaatt acgcaataga aaattcaaaa ggatactcga agactttagc atagttccaa
      421 agtccactaa accaagcccg tcttttttta ctaagtgcta tttttaatac cagcttgtcg
      481 tttaattttt caaagtaagc tcctattgca aaagttctgt agcgtagtgt tgataatgtt
      541 ttttgagttt tttcctgcaa cacaaatgtt ctaaaaagtg ccattaaatt ataggctatc
      601 atagcaaaag ttaaagctgc ttctgtagca taaaagtctt taagattaaa actgtcaaag
      661 ccaaaatcat atttcagttc tttaattcta ttttctgcat cgcctcttcc tctgtagagt
      721 ctccaaattt ctgcaggtgc aagttttaga ttggttaggt atgcggagta gcgataattt
      781 ttatagattt cttgttcagc aaacaagccc aattgtttgc ctgtggcttt tggcctgtct
      841 tttagtgttt gccttacaat aactaatcgt cttgattttg tccaggaatc gctttggtat
      901 agctgttcac aaatttcaat tcctgtatcc agcaaaatcc aattattact tgccgcaata
      961 agcctttgta tgggctgcga aaactttgct gccactacat aatccattgt tttaaattct
     1021 aaataatcta aaatgtttga ctgaaaaaag ccactgtcta atcttattaa actaacagtt
     1081 ttgtttttta atttggataa agtatcctct aaaaaaccaa gaaaattatt agccgaagat
     1141 gtatcgccac tgcgaagcca catattggct accagtttta catcagcaat aaaagcaatt
     1201 aatggatgat gtgatggcct tcctctttta gctggattgt atcctttttt tgcgccttgc
     1261 tgaaccccat aacgggtcat tactgacgaa tcaatatcta atgtaaaatt atcaaacttg
     1321 aaattatcaa aaatccacga gtaaaaatat tggctgatat tatggttttt agcctgtgta
     1381 aatttgctga aaaaccgttt gtaagtatct tgtccagggg tatttttcca gtcgaatatt
     1441 ttacccaaag cactatcatg acgggtaact tctgtatgta aaaacctatt ggcaccacac
     1501 cagatactgg tgatgaagcc ctcaataatg gtagaggttt tatagcctct gttagaacca
     1561 gattgaggta agtcggtatt ttgatcaata agttctctaa agcctgtttt ctgcaacatt
     1621 tgcttcaaaa aaaccattcc gccccatggc gtaatctctt tactggtata gcttatatca
     1681 aattccattg gtctgtttta tctaatgcta cgcattagaa tgtaaatagc taaagtaatt
     1741 cctttttgct attgcggaat ttgggtttaa cagcgatggc gcaatttata aaagatactg
     1801 taaagaattg agaaacaagc ttgccggata tccggacact ttgtttcata agtttcatag
     1861 acgttgcagg ccacctttat ttacccggga tgttcacaac cagccttggg ttagaagtat
     1921 cagtgcaagg atttttgcag aatgaaaggg ttaattgaag taagtattag ttaattatat
     1981 gacgttaacg gacgccagcg aagggataat ggaagaaatg aattaaatag ctgttatctt
     2041 gtaccactgt gggtgttctc tattgcaatt caaaacattt tactatcccg accactcttg
     2101 cagcgaatag tcaagtcaa
//
LOCUS       DASVIM010000147         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1680260, whole genome shotgun sequence.
ACCESSION   DASVIM010000147 DASVIM010000000
VERSION     DASVIM010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680260"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VF305_02995"
     CDS             <1..647
                     /locus_tag="VF305_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX7416133.1"
                     /translation="NFIDFFIHLDRYLPIIIDSFGIWAYVIVFLVIFCETGLVVTPIL
                     PGDSLLFALGTFAALGYLNIEALFILLSIAAIAGDTVNYTIGHFIGPKVFNYEDSRFF
                     KKEYLRKTHEFYEKHGGKTIIIARFMPIIRTFAPFVAGVGAMRYSRFILYNVAGGIAW
                     ICTFLLGGYFFGNIPAVKNNFTMVVVAIVIVSVMPGFIEYWRQKRYSSHKKVGN"
     gene            669..1514
                     /locus_tag="VF305_03000"
     CDS             669..1514
                     /locus_tag="VF305_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX7416134.1"
                     /translation="MKFIYFAVFLILLFLSAFSAEANNIIKIGGDVTIEEQQKINNVV
                     VVGGQITINGLVENNVVAVGGSIVLTNKAVVRGNVVCIGGVIAKGNGSQVYGDITEIN
                     SSNISSAIASVLSGDWEGWSLIINFIYLCFFAVIFIIALLMAILIPRPLIAIANMIQT
                     NKTKSFFWGFFATLTIPPFFFLLIISIIGITLIPLIFAALLLAFVLGFIGVSALIGNF
                     ILTKIFRKHKKSLVQETILGLILLWSIGWLPYIGWMVKVIAITFGLGGVLLILFNRKH
                     QSATP"
     gene            1772..>2399
                     /locus_tag="VF305_03005"
     CDS             1772..>2399
                     /locus_tag="VF305_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416135.1"
                     /translation="MTVKADQKPIEMDADFPKSLYQRYMQAAAVRSIASFVMWLTALT
                     AYLFGGIHFDNLASVSIAVLYLILMNPPTLWVLKRIKNKNLAEYFSYFISLLEIIGFT
                     AIIHSIGGIEAAFLTPIYAASIAYVGPMEPRKTPFIIAGLSSICFCLLLILEHFGILQ
                     TLKVNPDYYMPWSYQVAIMFVEVPLLFVVAFVSSYTTQLLKKNREKLHR"
BASE COUNT          653 a          471 c          460 g          815 t
ORIGIN      
        1 taaattttat agattttttc attcatcttg accggtatct gccgattatc attgatagct
       61 ttggtatctg ggcttatgtt attgtatttc ttgttatttt ctgtgaaact ggccttgtgg
      121 ttacacctat tttgcccggc gattcactat tatttgcttt gggaactttt gccgcactag
      181 gatatttgaa tattgaagca ttgtttattt tactctctat cgccgccatt gccggagata
      241 cagtcaatta cactatcggc cattttatcg gtccgaaggt ttttaattat gaagacagcc
      301 gctttttcaa aaaggaatat ctccggaaaa cacatgagtt ttatgaaaag catggcggta
      361 aaacaataat tattgcccgt ttcatgccga ttatccgtac ctttgctcct tttgttgccg
      421 gtgtcggcgc tatgaggtat tcccgattca ttttatacaa tgtggccggt ggtattgcgt
      481 ggatttgtac gtttcttttg ggtggttatt tttttggcaa cataccggct gtgaaaaaca
      541 attttaccat ggtcgtcgtt gcgattgtca ttgtttccgt aatgcccgga ttcattgaat
      601 actggcgaca aaaacgttat agttcccata agaaagttgg caactaaaaa atgaggaaaa
      661 gtatcagcat gaaattcatt tattttgccg tttttttgat attgcttttt ctatcggcat
      721 tttccgcaga agcaaataac ataataaaga taggcggcga tgtgaccata gaagagcaac
      781 aaaaaataaa caatgtagtt gttgtcggtg gtcagataac cattaacggc cttgtagaaa
      841 ataatgttgt tgccgtcggt ggatcgattg tgcttaccaa caaagctgtg gtgcgcggca
      901 atgttgtttg cataggcggt gttattgcca aaggaaacgg ctcgcaagtc tacggtgata
      961 ttactgaaat aaattcttcc aatatatcca gtgccattgc ttccgttctc agcggagact
     1021 gggagggctg gtcattaatt attaatttta tttatctgtg cttttttgcc gtcatattta
     1081 tcatcgccct gctaatggcg attttaatcc cgcgcccact gatcgccatc gctaatatga
     1141 tccaaaccaa taaaacaaaa tctttttttt ggggattttt cgcaaccctg acgattcctc
     1201 cttttttctt tttgctgata atttcaatta taggcattac tttaatacca ttaatatttg
     1261 ccgctttatt gctggcattc gtacttggat ttataggcgt aagtgcgctt attggtaatt
     1321 tcatcttaac aaaaatattt cgaaaacata agaaatctct agtgcaagaa accattttag
     1381 gattaatttt actttggtcg ataggctggc taccttatat aggctggatg gtaaaagtga
     1441 tcgctataac atttggcctg ggcggcgttt tgctcattct ctttaaccgc aaacatcaaa
     1501 gcgcaactcc atagggactt ctgaagctcc tgtaaaatcc gctacttcgc aaacctattt
     1561 gccacactta tttaaagtct ctggtttggt gaaaaaaatt gtggtttaat tttcttgaca
     1621 cgttaacgcc tttttcctaa ttaataagga atataaaaca atttcttgtc aaaaacagcc
     1681 tcccgcagat ttctttgata ttgttctata aaaagtaagg caagaattat aaaaatatta
     1741 tagcaaacct caaaaaagcg aaatagtata aatgactgtc aaagctgatc aaaaaccaat
     1801 agaaatggat gccgattttc caaagtcact ttatcaaagg tatatgcagg cagccgcagt
     1861 aagatcaatt gccagttttg ttatgtggct aactgccttg acagcttatt tattcggcgg
     1921 cattcatttt gataatttag ccagcgtgag cattgccgtt ttatacctga tcctgatgaa
     1981 cccgcctacc ctctgggttc tgaaaagaat taaaaataaa aatcttgccg aatacttttc
     2041 ttatttcatc agcttactcg aaattatcgg gtttaccgcc attatccatt ctattggcgg
     2101 cattgaagcc gctttcctga cacccatcta cgcggcctct atcgcctatg taggtcccat
     2161 ggaacctcgc aaaacgcctt tcatcatcgc tggtttaagc agtatttgtt tctgtcttct
     2221 gcttatttta gaacacttcg ggatccttca gactttgaaa gttaatcccg attattatat
     2281 gccctggtca tatcaggttg caataatgtt tgtggaagta cctttgttgt ttgttgtggc
     2341 cttcgtatct tcatacacga ctcaactatt gaagaagaac agagagaaac tgcaccggc
//
LOCUS       DASVIM010000148         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_485494, whole genome shotgun sequence.
ACCESSION   DASVIM010000148 DASVIM010000000
VERSION     DASVIM010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485494"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1390
                     /gene="rdgC"
                     /locus_tag="VF305_03010"
     CDS             320..1390
                     /gene="rdgC"
                     /locus_tag="VF305_03010"
                     /inference="COORDINATES: protein motif:HMM:NF016281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEX7416136.1"
                     /translation="MSLLKGSFTFTRFHVDGQLPQAFLNFVNDRIKANAYREAPKSTQ
                     EKLLGWVSLTDVLDADFAQANYTLGDYLIFSLRIDRKLIPPKLMKIKLLEEERRFLAK
                     NDKKRINKQMAAGIKEKVELELLTKLDAVPSFYDVCWALRQNTVYFSSLADKVADDFV
                     ELFKKTFSLNLKRFLPQENPQLAKNNESAEGVSFIGREFLTWLWFKSEERNGRIALTN
                     SEEVELHFLKRIVLGSGEGEYSQSVVCSGIHAELKEGKEAIRQGKKVKEAGIKLTSNQ
                     NEWEFTFKADTFQFQSLKPPVMDWQETQEDPSGNILERIYLIENAVKTMDSLYESFLQ
                     IRYSPQWEESEMKLLTKWLEHK"
     gene            complement(1396..>1995)
                     /locus_tag="VF305_03015"
     CDS             complement(1396..>1995)
                     /locus_tag="VF305_03015"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HEX7416137.1"
                     /translation="IYWIARLYCETFRLKIENEDEWFNYLEQGGRVLLCGWHQQFFAG
                     IRFFKKYRKYRPSLMSSRSLDGQFAGGIAKRAGCYTVWGSSSRSGGTALREMIHRLRD
                     YRLAAHLLDGPRGPAGIVKVGAIAIAHGAGAVIIPVFVTADRAWYLHSWDRFMIPKPF
                     ARVTITFYPKIELPPVMDKADYENQRKRLEMIMQPCLQR"
BASE COUNT          599 a          435 c          407 g          554 t
ORIGIN      
        1 ccattttgcc ggcaatttcc acgctcaaaa aaactttgac ggctttttct tccagccact
       61 tttttaatga attggtgtac tgaacgattt ttgctttttc aatatgagca acaatgccta
      121 ctttcttgat tttcattgat ttaaacctca gaacatcgga atatgaagct gactaacata
      181 aaaaaaagcc gctgtctatg gataaatatt ttactgatat gtttagaaaa taattgatta
      241 ttcagggcaa ttccccagca taaattcttg acagattcag gtcatataat atacggtgca
      301 cctctgtgag gtgctaatta tgagtttact aaaaggtagt tttacgttca cgcgttttca
      361 tgtagatggt cagttgccgc aggcattttt gaatttcgtc aacgaccgga ttaaagccaa
      421 tgcctatcgc gaggcgccta aatcaactca agaaaaactg ttgggctggg tttctctcac
      481 cgatgtattg gatgccgatt ttgcacaggc taactacacg ttgggcgatt accttatttt
      541 ttctctgcgt attgaccgca agcttattcc acccaaactg atgaaaatca aattgctgga
      601 agaggaaaga cggtttcttg ccaaaaacga taaaaaaagg atcaacaaac aaatggccgc
      661 aggtattaaa gaaaaggtag agttggaact tctgaccaaa cttgatgccg taccttcttt
      721 ttacgatgtt tgctgggcgc tgcgacaaaa cacagtttat ttttcttctc ttgccgacaa
      781 ggtcgcggat gattttgttg agttgtttaa aaaaactttt tccttaaatc ttaaacgctt
      841 tttgccacaa gaaaatccgc aactagcaaa aaacaatgaa tccgcagaag gcgtttcttt
      901 tatcggccgt gaatttctaa cctggctttg gtttaaatcg gaagaacgca acggcaggat
      961 agcgctaacg aacagtgaag aagtcgaact tcatttcttg aaaagaatcg ttttgggatc
     1021 cggtgaaggc gagtattccc agagtgttgt ttgttccgga attcacgccg agctcaaaga
     1081 aggcaaggaa gccatccgcc aggggaaaaa agttaaagaa gcgggaatca aactcacttc
     1141 taatcaaaat gaatgggaat tcaccttcaa agccgatact tttcaattcc aatctttaaa
     1201 accgcccgta atggattggc aagaaacaca ggaggatcct tcgggaaata ttctggagcg
     1261 gatttactta attgaaaatg cggtaaaaac aatggatagc ctttacgaat cttttttgca
     1321 aatccgttat tcgccccaat gggaagaaag tgagatgaaa cttttgacca aatggttgga
     1381 acataagtga aggttctatc gctgcagaca aggctgcata atcatctcca acctttttct
     1441 ctgattttca taatcggctt tgtccattac cggcggcaat tcaattttcg gataaaatgt
     1501 tatcgtaact cgggcgaaag gtttaggaat cataaatcta tcccaactgt gcaaatacca
     1561 agctcgatcg gcagttacaa atacgggaat aatcacagca cctgcgccgt gggcaatagc
     1621 aatggcgcca accttaacaa ttcctgcggg accacgcggc ccatccaaga gatgcgccgc
     1681 cagccggtag tcccttaacc tgtgtatcat ttctctaagg gcagttccgc cactgcggga
     1741 agaagaaccc cagacggtat aacacccggc gcgcttcgca atgccaccgg caaattgccc
     1801 atccaaactt ctggaagaca ttaagctggg tctgtatttt ctatatttct taaaaaagcg
     1861 aattccggca aaaaactgct ggtgccaccc gcaaagaaga acccttccgc cctgttccag
     1921 ataattgaac cattcatctt cgttttctat tttcagacga aacgtctcac aatatagccg
     1981 tgctatccag taaat
//
LOCUS       DASVIM010000149         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1253794, whole genome shotgun sequence.
ACCESSION   DASVIM010000149 DASVIM010000000
VERSION     DASVIM010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253794"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1041
                     /locus_tag="VF305_03020"
     CDS             169..1041
                     /locus_tag="VF305_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7416138.1"
                     /translation="MIKIALILLKYIPLSLRKMIAYLLARFAYWLSLKHRLIALHNLS
                     RSFPEKKLEEIVKIAKKSYQNFSFVAAEFFDIPYLNKDNLHQWITLEGLENYTEACKE
                     GKGVLIFGAHFGNWEIGNAALAIMTQPFIFVYRIFDSPLIESVVTGVRASYGNVSLSK
                     ENAMRPTIRLLKKGITINLLIDQNASWQEGVFVDFFGRKACTTSGLSLLAMHSGAPVL
                     PAFTRSLPDGKYLLEIGKKVKIIHSGNRDNDVLINTQNFTKIIEEQIRKYPEQWFWVH
                     QRWKTKLSQVKQRG"
     gene            1046..>2016
                     /gene="waaF"
                     /locus_tag="VF305_03025"
     CDS             1046..>2016
                     /gene="waaF"
                     /locus_tag="VF305_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX7416139.1"
                     /translation="MKSGNIKKMPQQGINKILIRGTNWIGDAILTLPAVAAIRTTYPD
                     AYIAILVKPLVADVYELFSAVDEVIIYESKLDNPIGVFKLAHKLRKKNFDAAILLQNA
                     IEAAIIAFAAGIPLRAGYNSDGRGLLLTHRVQRTKEIRRVHQTDYYLEMVKALGCVSA
                     DREMHLEIKINRSEAQSILKKFIHGTNKAIIGIAPGATYGPAKRWFPERFAAIADKLC
                     DSYAIQGILLGGKSDWEIAQKVQKLAHTNLINLAGETTLSEAIHLISQCRLFISNDSG
                     LMHIAGALNIPTVAIFGSTNPVTTAPIGNKSTIVRREISCSPCLKET"
BASE COUNT          623 a          423 c          408 g          562 t
ORIGIN      
        1 aacccaaaaa gacggcgtac gcctccagga atttcccgaa tttctaaaca taatttacat
       61 gttgtgcata gaaatgacaa ttattccctc cagggaatta tttgaaaaat ttatcttaaa
      121 taaacttgct gtagcttagg aagcaaaggt tcaataagga tagatttatt gataaaaata
      181 gcattaattt tattaaaata tattccgttg agtttaagaa agatgatcgc ctatctattg
      241 gcaagatttg cttattggct ttctctaaaa catcggctta ttgcccttca taacttaagc
      301 cgttcttttc ccgaaaaaaa gttggaagaa attgttaaaa ttgctaaaaa atcatatcaa
      361 aatttttctt ttgttgccgc cgaatttttc gatattccgt atttaaacaa agataatctg
      421 caccagtgga ttacccttga aggcttggag aactacacgg aagcttgcaa ggaaggaaaa
      481 ggtgtgctta tttttggcgc tcattttggt aactgggaaa taggcaatgc ggctttggct
      541 ataatgacgc agccatttat tttcgtttac cgcatcttcg acagcccttt gatagaaagt
      601 gttgtcaccg gcgtgcgtgc ctcttacggc aatgtttcac tttctaaaga gaatgctatg
      661 cggccgacga ttcgcctgtt gaaaaagggt atcacgataa atctcctcat tgaccaaaac
      721 gcctcctggc aggagggtgt atttgtcgac ttttttggcc gaaaggcttg caccacatcc
      781 ggactatccc tattggctat gcattcagga gcgccggtgc tccccgcctt tacccgcagc
      841 ctgcccgatg gcaaatattt attagagatc ggcaagaaag taaaaattat tcattccgga
      901 aatcgcgata atgatgtatt aatcaataca caaaacttca ctaaaattat tgaagaacag
      961 attcgtaaat atcccgaaca atggttctgg gtacatcagc gctggaagac aaaactctcc
     1021 caggtaaagc aaagaggata aacaattgaa gtctggaaat atcaaaaaaa tgccgcagca
     1081 aggtataaat aaaatattga ttcgcggcac caactggatt ggcgatgcca ttttgacatt
     1141 gccggcagtt gccgccattc gcactacata ccctgacgct tacattgcca tacttgtaaa
     1201 accgttggtg gccgatgttt atgagctttt ttccgctgtg gatgaagtga tcatttatga
     1261 aagcaaatta gataatccca tcggtgtatt taagctcgcg cacaagctca gaaaaaagaa
     1321 ttttgatgcg gcgatacttt tacaaaacgc catcgaagcg gcgataattg ccttcgccgc
     1381 cgggataccg cttcgcgccg gatacaactc ggacggccgc ggccttcttt taacccatcg
     1441 tgtacagcgc actaaagaaa taagaagagt ccaccaaact gattattatc tggaaatggt
     1501 caaggctttg ggttgtgttt ccgcagaccg tgaaatgcac ttagaaataa aaatcaaccg
     1561 ttcagaagcc caaagcattt tgaaaaaatt tatacacgga acaaataaag ctataatcgg
     1621 cattgctccg ggagctacat acggtccggc taaaagatgg tttcccgaaa gatttgccgc
     1681 tatagcagat aagctttgtg atagttacgc tatacaggga attcttctag gcggtaaatc
     1741 agactgggaa atagctcaaa aagtccaaaa actggcgcac acaaatttaa ttaaccttgc
     1801 cggtgaaaca actttgagtg aagctattca tttgatttcc caatgccgtt tatttatcag
     1861 caatgattcc ggactaatgc atatagccgg tgcattgaac attccaactg ttgctatttt
     1921 tggttcaact aaccctgtta ccactgcacc gattggaaat aaatccacca tcgtacgtcg
     1981 agaaatatcc tgcagccctt gcttgaaaga aacatg
//
LOCUS       DASVIM010000150         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1680771, whole genome shotgun sequence.
ACCESSION   DASVIM010000150 DASVIM010000000
VERSION     DASVIM010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680771"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VF305_03030"
     CDS             <1..837
                     /locus_tag="VF305_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX7416140.1"
                     /translation="DPVEICTQAVERAKKEGYEVVIIDTAGRLHIDEVLMKELQNIKE
                     KVNPAEILYVADAMTGQDAVNVGNKFNEIIGIDGVIMTKMDGDARGGAALSLKAVIGK
                     PIKYAGIGEKIDALEVFHPERMASRILGMGDILTLVEKAKATIDEKAALQLEKKIRKN
                     EFTLEDFRNQLVQIRKMGSIKDILGMIPGLGKVKALKDINPDDKELVKVTAIIDSMTL
                     KERSNCRIIDGRRRKRIALGSGTSVQDVNRLLKNYAEIKKMMKTFSPKEGATSLMRNF
                     PF"
     gene            908..1162
                     /gene="rpsP"
                     /locus_tag="VF305_03035"
     CDS             908..1162
                     /gene="rpsP"
                     /locus_tag="VF305_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX7416141.1"
                     /translation="MAVKIRLTRMGAKGKPFYRIVAADTDFPRDGRFLEILGNYDPKK
                     NPAEITVKEERVRDWLAKGAKATLTVANLLKTKGIEARVK"
     gene            1305..1535
                     /locus_tag="VF305_03040"
     CDS             1305..1535
                     /locus_tag="VF305_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX7416142.1"
                     /translation="MKELVKYIAQSLVDTQDKVEVTEIVGEQTSVIELRVAKEDLGKV
                     IGKQGRTAKAIRTILSAASAKIHKRAVLEIIE"
     gene            1542..2042
                     /gene="rimM"
                     /locus_tag="VF305_03045"
     CDS             1542..2042
                     /gene="rimM"
                     /locus_tag="VF305_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX7416143.1"
                     /translation="MNLFAIGEIVKTRGLRGCMKVVSFVDTKDISAELEFIYIQDNSG
                     QKKLYNLRKIDISGKFLFIELDAIDDVDLAKKFVGCKIVIPRKMLKELPEGEYYWRDI
                     IGLDVYTEEGKLLGQIESVFPTGSNDVYVCKGGQREILLPAIADVIKSIDIDRRVMNV
                     KLMEGL"
     gene            2047..2784
                     /gene="trmD"
                     /locus_tag="VF305_03050"
     CDS             2047..2784
                     /gene="trmD"
                     /locus_tag="VF305_03050"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX7416144.1"
                     /translation="MIKFDVLSIFPEMFASPLSFSLLKKAQEKGFLNICLHDIRNWAE
                     DKHKMTDDAPYGGCCGMVMKIEPVEKALAAIKSQGVESLVVLMTPQGETFNQKIAAEL
                     AEKKQIIIICGRYEGVDERIREHLTDREISICDYILTGGELSALVLIDAVSRLIPGVL
                     GNEESTLSESFSPGLLEYPQYTRPAEYNGWGVPEVLVSGNHAEIERWRRYESLKRTYK
                     RRPDLLEKTELSEVDKKNLQKIKAEDI"
     gene            2948..>3466
                     /locus_tag="VF305_03055"
     CDS             2948..>3466
                     /locus_tag="VF305_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX7416145.1"
                     /translation="MLYIALLHYPVYNKDGEVVTTAIANMDIHDIARCASTYDVRGFY
                     IVNPISAQRDLAKDIISHWRDGYGANFNPSRKDAFQLVRLKQNLEQVLTEIEGQTGSV
                     PKTIVTGANLKEELLSFRELKKILRNDDLPYLLIFGTGSGIAKELVNKADFRLEPIIG
                     VKKYNHLAVRSAA"
BASE COUNT         1132 a          578 c          837 g          919 t
ORIGIN      
        1 gacccagtgg aaatttgtac tcaggcagtc gaacgggcca agaaagaagg ctatgaggta
       61 gtcattattg acaccgccgg aagattgcat atcgatgaag ttttgatgaa agaactccaa
      121 aatataaaag aaaaggtcaa tccggcggaa attctttatg ttgctgatgc aatgactgga
      181 caggatgctg ttaatgtagg caataaattc aatgaaatta taggtatcga tggtgtcatc
      241 atgacgaaaa tggatggtga cgcgcgaggc ggggcagcgt tatccctcaa ggcagttatt
      301 ggaaaaccta taaaatatgc gggtataggc gaaaaaattg atgctctgga agtttttcat
      361 ccggaaagaa tggcttcgcg cattttgggt atgggcgata tcctgacttt ggtggaaaaa
      421 gcaaaagcca caattgacga aaaagctgcc ctgcaactgg agaaaaaaat aagaaaaaat
      481 gaatttacac tggaagattt tcgcaatcaa ttggttcaaa tcaggaaaat gggctcaatc
      541 aaagacatct tggggatgat ccccggttta gggaaagtta aggcgctaaa agatatcaat
      601 ccggatgata aagaactggt taaagtaaca gcaattatcg attccatgac gctaaaagaa
      661 cgctctaatt gccggattat cgacgggcgc cggcgaaaaa gaattgccct gggcagcgga
      721 acatcagttc aggatgtaaa tcgtttattg aagaattatg cagaaattaa gaaaatgatg
      781 aaaacattta gtccaaaaga aggtgcgact tcgctgatgc ggaattttcc cttttaatat
      841 gaaaaaaact gtgatagctt aattctttac gtaaaattat attttaagga ggtttcattt
      901 aaaatcaatg gctgttaaaa ttcgactaac tcgtatgggt gcgaaaggca aaccctttta
      961 tcgcatagtg gcagcggata cagattttcc ccgtgatgga agatttttag agattttagg
     1021 caattacgat cccaaaaaaa atccggcgga aattaccgtg aaagaagaac gtgttcgcga
     1081 ctggttggcg aaaggcgcta aagcgacctt aacagtagct aatttattga aaacaaaagg
     1141 tatcgaggct agagttaaat aacggcagct ttgatgtttt gataattagg atagggcagg
     1201 gtgacagttt ttttatatgg ccaggaagcg tttaagccat ggagttattt atcgaaaaaa
     1261 tgatttcccc aaagaagtaa tcaaaatcag tggaggttat ggcgatgaaa gagttggtta
     1321 agtatatagc tcagtctttg gtagatactc aggataaggt ggaagtgact gaaatcgtcg
     1381 gcgagcaaac atcagtgatt gaattgcgtg tggccaagga agatttgggt aaagtaattg
     1441 ggaaacaggg cagaactgcc aaggctatcc ggacaatttt gagcgccgct tccgccaaaa
     1501 ttcataaacg ggcagttcta gaaattattg agtaggggaa aatgaatctc tttgcaattg
     1561 gggagatcgt aaaaactcgc ggtctccgcg gctgcatgaa agtggtatcc tttgtggata
     1621 ctaaagatat ctctgccgaa cttgaattta tttatattca ggataattca gggcaaaaaa
     1681 agctttataa tcttagaaaa atagacattt ccggaaaatt tctgtttatc gaactagacg
     1741 ctattgatga tgtggatttg gcgaaaaagt ttgtcgggtg caagatagta ataccgagaa
     1801 agatgcttaa agaactgccg gagggtgaat attactggag agatatcatc ggtcttgatg
     1861 tctatactga ggagggaaaa cttctcggcc agattgaatc agtctttccc acaggcagca
     1921 atgatgttta tgtctgcaaa ggaggccaaa gagagatact tcttccggca attgccgatg
     1981 taatcaaatc aatagatatt gatcgaagag ttatgaacgt aaaactaatg gaaggacttt
     2041 aaaaaagtga tcaaatttga tgttctttcc atttttcccg agatgtttgc ttctcccctc
     2101 agttttagcc tgttaaaaaa agcccaggaa aaaggttttt taaatatttg cctgcacgat
     2161 atccgtaatt gggctgaaga taagcataaa atgactgatg acgcccctta cggcggctgc
     2221 tgcggtatgg tcatgaaaat agagcctgta gaaaaggcgc tggcggcgat caaaagccag
     2281 ggagttgaat ctctcgttgt tctgatgacg cctcagggag aaacttttaa tcaaaaaatt
     2341 gcggcggagc tggccgaaaa aaaacagata attattattt gcggccgtta tgagggtgtc
     2401 gatgaaagaa taagagaaca tttaaccgat cgggaaatat ctatctgtga ttatatttta
     2461 accggaggcg agctttccgc tttagtttta attgatgccg tctcccggct tattccgggt
     2521 gtattgggca atgaagaatc aacgctgagc gaatcttttt ctcctggcct tttggaatat
     2581 cctcaatata caagaccggc ggaatataac ggttggggag tgccggaagt tttagtttcc
     2641 ggaaaccatg ctgaaattga acgatggcgc aggtatgaat cgctcaaaag gacatataag
     2701 cgccggccgg atttactgga aaaaaccgaa ctttcagagg tagataaaaa aaatctgcag
     2761 aaaataaagg cggaagacat ataaaatatt gattttgaga gccgtttgcg gtagtaaaac
     2821 taacttttat tcaatgacac tcgctgaaaa cgaatgccgt gaagtttaaa acgttagtcg
     2881 aatcgggacg ggaaggttca tacggaaact attttccatt aaaatatata aaaccagaaa
     2941 agcgcttatg ctttacatag ctcttttaca ttatccggta tacaacaaag acggagaagt
     3001 tgtaactacg gctatcgcga atatggatat tcatgatata gccagatgcg caagtaccta
     3061 cgacgtgcgg ggtttttata tagtaaaccc gatatctgcc caacgcgatt tggcgaagga
     3121 tatcatcagc cactggcggg atggttacgg agcgaacttc aatccatcgc gcaaagatgc
     3181 ttttcaactg gttaggctaa agcaaaatct cgaacaggtt ttaacggaaa ttgaagggca
     3241 aacgggatct gttccgaaaa cgattgtaac cggagctaac ttgaaagaag agcttttatc
     3301 atttagagag ctgaaaaaaa tattaagaaa tgatgatttg ccttatcttt taatatttgg
     3361 tacaggttcg ggaattgcta aggaattggt aaataaagcc gatttccgcc tggagccgat
     3421 aataggagtt aaaaagtata atcatcttgc cgttcgttcg gcggcg
//
LOCUS       DASVIM010000151         1903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_912868, whole genome shotgun sequence.
ACCESSION   DASVIM010000151 DASVIM010000000
VERSION     DASVIM010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912868"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1117)
                     /locus_tag="VF305_03060"
     CDS             complement(242..1117)
                     /locus_tag="VF305_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7416146.1"
                     /translation="MPENVLKKEMPDLKMQDVGGAELPYLYYEGEAPQILFVHATGFL
                     PWLWHPVIEELAPPSQVWVPYICNYRECDPEKGGLSWDIIAKDLSVFCRSQQIKKPLF
                     VGHSMGGTVLTIAAASYGIEPSGMILIEPIFLPEEFYSMKATVKDHPLASKSIKRTNH
                     WKNEEEAWAYLKSKSLFSDWDEQVLQLYFKYGMEKQKEGDLKLACTPESEAAMFMGGW
                     SRNPWPLLDKLTCPVLVVEGEKSENKNFIDLKKAVSLLRYGKYKSVDGAGHLIPMQKP
                     KIIAQIIKEFLAEAI"
     gene            complement(1155..1703)
                     /locus_tag="VF305_03065"
     CDS             complement(1155..1703)
                     /locus_tag="VF305_03065"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HEX7416147.1"
                     /translation="MDDFVPRRIFFTKGVGTHKDELHSFERALRDAGIEKCNLVQVSS
                     IFPPRCKMISKTQGLKELIPGAITYCVMSRSCTNEPKRLLAASVGCAIPADKASYGYI
                     SEHHAYGQTEKQAGDYAEDLAAAMLASTLGIDFNVDESWDEKKEIFKISSKIVRTLNV
                     TQSTICRDNQYTTVMAAAVFVF"
BASE COUNT          475 a          454 c          376 g          598 t
ORIGIN      
        1 tgctttgctt tcagaacttt gctgattatc tgcagtaaga gccattaata ggtttcccct
       61 tattggtgta attccacaac caaggtggcc atcgccaacg ctacgctaac catcctacca
      121 agtttcatca tttttatctt cttttgtctg tggatcaaca ggcctcaatc ctgtagccga
      181 ttggccttcg aatattgcga aatgaaattt ggcatattta tcgaaattcg tcaactgtct
      241 cttaaattgc ttccgccaaa aattctttta tgatttgcgc gattatttta ggtttttgca
      301 tggggatgag gtgtccggct ccgtcaactg atttatattt tccatagcgc aataaggata
      361 ccgccttttt caaatcgata aaatttttat tttcgctttt ttctccttct acaaccaaaa
      421 ccggacaagt taatttatcg agcagtggcc agggattcct gctccagccg cccataaaca
      481 tcgcggcctc actttccggc gtgcaggcaa gtttcaaatc tccttctttt tgtttctcca
      541 tgccatattt aaagtatagc tgtagaactt gctcatccca gtcggaaaaa agagattttg
      601 attttagata agcccatgct tcctcttcat ttttccagtg atttgttctt tttatcgatt
      661 tggaagccag tggatgatct ttcacggtgg ctttcatgga gtagaactct tccggcagga
      721 aaatcggctc gattagaatc atgccgctag gctctatacc gtaactagcg gcggcgatgg
      781 tcagtactgt accgcccatg gagtgtccga caaacaaagg ttttttaatc tgctgcgagc
      841 ggcagaaaac ggataaatct ttggcgataa tatcccaact tagaccgcct ttttccggat
      901 cacactcgcg ataattgcag atatacggca cccaaacctg acttggcggc gccaactcct
      961 ctattaccgg atgccagagc cagggcaaaa aacctgtggc atggacaaaa agaatttgcg
     1021 gagcttctcc ttcatagtaa agataaggca actcggctcc gccgacatcc tgcatcttta
     1081 aatccggcat ttcttttttt aaaacatttt caggcataca attattgctg ttgtcaattc
     1141 ctatcagcca atatttaaaa gacgaacaca gccgcggcca ttaccgttgt gtattgatta
     1201 tccctgcaaa ttgttgattg ggttacgttg agggtacgaa caatcttgct gcttatttta
     1261 aaaatctcct ttttttcatc ccagctttcg tccacattga aatcaattcc taaagtggag
     1321 gccagcatgg ctgcagccag atcttccgcg tagtcacccg cttgcttttc ggtctgacca
     1381 tatgcgtgat gctcgcttat atagccataa gatgctttgt ccgcgggaat ggcgcaaccg
     1441 accgatgcgg ccagaagcct tttgggttca ttggtgcagg aacgactcat aacacaatac
     1501 gtaatagcgc cggggattaa ctctttgagt ccctgagtct tagaaatcat tttgcagcgg
     1561 ggcggaaata tgctggatac ctgtaccaaa ttgcattttt ctattccggc atctcttaag
     1621 gcacgttcaa aggaatgcaa ttcatctttg tgcgtgccga ccccttttgt aaaaaaaatt
     1681 cttctgggta caaaatcatc cacttttcaa ctcctttttt tgctgtaagc ggcaaaagat
     1741 tttatttttt taacaagtcc ggctatagac tatcccccgt cggtctgaat aaaagcagta
     1801 ttcgcgtata aatactccac cttttctttg tgcttcagct cttgagccgc cattttcatc
     1861 agcatatctt ttatttctcc gtcaggatga atggctgcca gcc
//
LOCUS       DASVIM010000152         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_658780, whole genome shotgun sequence.
ACCESSION   DASVIM010000152 DASVIM010000000
VERSION     DASVIM010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658780"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VF305_03070"
     CDS             <1..415
                     /locus_tag="VF305_03070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7416148.1"
                     /translation="LGDDCMLAQDVYITDSDWHDLYDRTLSIGNTHEVKIGNNVWIGD
                     SAIVCKGVTIGDNAIIGAGAIVVKDIPANAIAAGNPAAVIRYLDPDKQLKSRSQWMGE
                     PDKLTKFYDSVERVMREGNTLLGWFRTLLFPRKGD"
     gene            420..1991
                     /locus_tag="VF305_03075"
     CDS             420..1991
                     /locus_tag="VF305_03075"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7416149.1"
                     /translation="MRIAIVATPYPLEEIPSPPLGVTYVASAFEAAGAEVRIFDYIVS
                     GYSKEKLKNQLEDFQPDAVGATSVTMNFYEAQQILRDVKNCNPEIVTMMGGPHVSFTA
                     METLRKYPEIDLIAIGEAEDTIAQLSPVLKQQNKWHDIRGIAYRNNDEIVITGKRDFI
                     SDIDQIPMPARHLLPLSRYRALGFPVSIITGRGCPNSCIFCLGRKMVGSKIRRRKVKY
                     VLDEMEQIIGCGFERINIADDLFAVDKDRVQEICSGIKERGLKFSWSAFARVDTVEQE
                     MLDAMADAGCDSVSFGVETGNPEMLKRIKKGIKLEQVGSAVKMCKQAGIIAHASFMVG
                     LPGETKETLVQTDQLARSLNILYGYHYLAPFPGTSVREKVGEYDLEILTDDWAKYDAN
                     DAIVKTSALEPDDIRAFVASYDEEMDTDWQELLQGYHDGTNTPEDNMRVEGHWRMKLT
                     YQVLKGDLIEKYGFIEPNLFQGSKDRALRELCQRIIIGTNADSRVVNNTISDFVNRGY
                     LQTAISDRGCNWRWT"
     gene            complement(2220..>2786)
                     /locus_tag="VF305_03080"
     CDS             complement(2220..>2786)
                     /locus_tag="VF305_03080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7416150.1"
                     /translation="REAHYLYKLTLNASKTGPCLEIGSYCGKSAVYLGTACKENGSIL
                     FSLDHHIGSEEQQPGEEYFDPELLDQETGKIDTLRFFRKTIAEFGLENTVIPIIGHSE
                     IVGRFWKIPLSLIFIDGSHAYESVLNDYKIWAKHLIIGGYLLFHDIFSDPAKGGQAPY
                     MVYQIAVASGSYEEMPMCESLGILKRKK"
BASE COUNT          778 a          578 c          673 g          757 t
ORIGIN      
        1 tttgggtgat gactgcatgc tggcgcagga tgtctatatt acggactcgg actggcatga
       61 tttatatgac cgcacgttat ccatcggaaa cactcatgaa gttaaaatag gtaataatgt
      121 ctggattgga gacagcgcga ttgtttgtaa gggcgtgact attggcgata atgccatcat
      181 cggagcggga gctatcgtgg tgaaagacat tcccgctaat gccattgccg ccggcaatcc
      241 cgcggcggtg ataagatatt tggatccgga caaacagcta aaatcaagat cgcaatggat
      301 gggcgagcct gacaagctta ctaaatttta tgactctgtg gaacgtgtga tgagggaagg
      361 taacacactc ttgggttggt tcagaacgtt actttttcca cgtaaaggag attgattcct
      421 tgcgcattgc tatcgtcgcg acgccctatc cattggaaga aattccctca ccgcctttgg
      481 gtgtaactta tgtcgcctct gcattcgaag ctgccggagc cgaggtacgg atatttgact
      541 atattgtttc cggttattcc aaagaaaaac ttaaaaacca actggaagat tttcagccgg
      601 acgctgtcgg cgcaacatct gtaacgatga atttttatga ggcgcagcaa atcctccggg
      661 atgtaaaaaa ctgtaatccg gaaattgtta cgatgatggg cggaccgcat gtatcattta
      721 cggcaatgga aacgctacga aaatatccgg aaatagactt gattgccata ggcgaggccg
      781 aagataccat cgcacaactt tcaccggtgt taaaacagca aaacaaatgg catgatatcc
      841 gcggcattgc ctaccggaat aatgatgaaa tcgtcattac gggcaaaaga gattttatct
      901 ctgatattga tcaaatacca atgcctgcac gtcatttgtt gcccctttcg cgttaccggg
      961 ccttgggctt tccggtcagc attattaccg gccggggttg tcccaattca tgtatttttt
     1021 gcctgggacg taaaatggtg ggttcgaaaa tccgccgcag gaaagttaag tatgttctgg
     1081 acgaaatgga gcagataatc ggttgtggtt tcgagcgcat caatatcgct gatgatcttt
     1141 ttgccgtcga taaagatcgg gttcaagaaa tatgcagcgg aatcaaagag cggggattaa
     1201 aattcagttg gagcgctttt gctcgtgtgg ataccgtaga gcaagaaatg ttggacgcga
     1261 tggccgatgc aggttgtgat agcgtcagtt tcggggtgga aaccggtaat ccggaaatgc
     1321 ttaaacgaat aaaaaaggga ataaagctgg aacaagtcgg ctcggcagtg aaaatgtgta
     1381 agcaggcggg cataattgcc cacgcgtcat ttatggttgg tctgccgggc gaaacaaaag
     1441 aaactctggt ccaaacagac caattagcgc gaagtttaaa catactttac ggctatcatt
     1501 atttggcgcc gtttcccggc acgagtgtgc gggaaaaagt cggagagtac gatttggaaa
     1561 tattgaccga cgattgggca aagtatgacg ccaatgacgc cattgtaaaa acttccgctc
     1621 tggagccgga tgacatacgc gcatttgttg ccagttatga tgaagaaatg gatactgact
     1681 ggcaagaact tttacaaggt tatcatgacg gaacaaatac gcctgaagat aatatgcggg
     1741 tggaaggcca ttggcgcatg aagctgacat atcaagtatt gaaaggtgat ctcatcgaaa
     1801 aatacggttt tattgagcct aatttatttc aaggttctaa agatagagca ttgcgggaac
     1861 tctgccaaag aataattatc ggcacaaatg cggattcccg ggtagttaat aataccatca
     1921 gtgattttgt taatcgcggt tatctgcaaa cagcaatttc cgatcgtggg tgcaactggc
     1981 gctggactta atttagagct ttgaaaaatc ggctcattcg tgctcagagt cttttttcga
     2041 ccatgtagcc tttctcgctt cgctacaaac gcaaacctct ccgccacggc ggatcagaca
     2101 ttgtgtttgc gcgcgcgaag cttcaaggtc atggtgctcg aaaaaatctt cgattcgctc
     2161 ttcatgagcc atttttcagc tattcgaaga tctagattaa taacatgttg gcttagctat
     2221 tatttttttc gttttaatat ccccagtgat tcgcacatcg gcatttcttc atatgatccc
     2281 gaagctactg caatttgata aaccatataa ggtgcctggc cgcctttggc cggatcggag
     2341 aaaatatcat ggaaaagaag atagccgcca atgattaaat gttttgccca aatcttgtaa
     2401 tcgttaagga cggattcgta agcgtggctt ccgtcaataa aaatcaagct cagaggtatt
     2461 ttccagaatc gtcccactat ttctgagtgg ccgataatcg ggattaccgt attttcaagg
     2521 ccaaattcag caatcgtttt gcggaaaaat cttaaagtat ctatcttccc tgtttcttga
     2581 tccagcaatt cagggtcaaa atattcttcg cccggctgct gttcctccga gcctatatga
     2641 tgatcaaggg aaaatagaat cgatccattt tctttacagg ctgtgcctaa ataaaccgct
     2701 gatttcccac aataactgcc gatttccagg cacgggcctg ttttactggc attcaaagtt
     2761 agtttataaa gataatgagc ttcgcg
//
LOCUS       DASVIM010000153         2755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_914700, whole genome shotgun sequence.
ACCESSION   DASVIM010000153 DASVIM010000000
VERSION     DASVIM010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914700"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VF305_03085"
     CDS             <1..243
                     /locus_tag="VF305_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416151.1"
                     /translation="YVDNNIIFTKEAMDKELSKVTDTFAAFDFAIKRELDSILYYHEI
                     KKLVPATQHAILDQIIDEERKHFALLTEMKKRFRKQ"
     gene            complement(313..1674)
                     /locus_tag="VF305_03090"
     CDS             complement(313..1674)
                     /locus_tag="VF305_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7416152.1"
                     /translation="MHKLSILIVEDGQSQREMLRDFLLSEGSTVAEAENGEIAIKKIL
                     SNHFDLILLDYKMPGMDGLEVLKEIKRINPEIDVVIITAYGTIETAVEAIKVGAIDYI
                     TKPVELDELLILVNRVAERRQLIRENELLREELGKKGVTADKIVYKSPRMAEVINMAG
                     RVAASKASVLIQGESGTGKELLARLIHQLSTRAHKPIIVVNCVALHENLLESELFGHE
                     KGAFTGATFRRIGRFEEADGGTLFLDEIGELPPTVQVKLLRFLQEREFQRLGSNVDIH
                     VDVRVISATNRDLGVQVKEGTFREDLFYRLKVVTMSLPPLRERKDDLPILIDHFIDKF
                     AKENGKNIQGLTAEARDSLLKYDYPGNVRELVNILERAVVIARDEYITTEDLPFKSAS
                     PSRDSGKKSASLLRESVEELEKKLIAESMSKAQDNQTKAAEILGMSERMLRYKLKKYG
                     LKN"
     gene            complement(1705..>2755)
                     /locus_tag="VF305_03095"
     CDS             complement(1705..>2755)
                     /locus_tag="VF305_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7416153.1"
                     /translation="NDQEKLLSSVGRPMPGLKNSDFDFEGILTGKIKIASRKLNYEED
                     KILDLAAPLSLDKKVVGIVRIGLDRGSMDKIIAENRQSIFIFLFFVVIITLLSMWLLY
                     HDQNRHLAGIVEMERRLEKAERLSSLGQLAAGVAHEIRNPLNAISMATQRLKRDFVPL
                     DSNRADDFQNLSSVIRDEIKRLNGIIEEFLAFSKSRRLELHNFSVTEVLQKIVSLIRE
                     EASARNVVIETKWRHTPAIIPMDINKLQQAFLNLIKNAMESMPAEGKIIITVDKEGKK
                     YIVVSIEDTGCGMTAEEIERIFNPEYTTKEKGLGLGIPLASEIIRGHGGDIRVISREG
                     EGTTFEVVLPVKDSA"
BASE COUNT          720 a          661 c          509 g          865 t
ORIGIN      
        1 tacgtggata ataatataat ctttactaaa gaagccatgg ataaagaact gtctaaggtg
       61 accgatacct ttgccgcctt tgattttgcc atcaaacgtg agttggattc aatcctctat
      121 tatcatgaaa tcaagaaatt agtgccggca actcagcatg ccatacttga tcagattatt
      181 gacgaggaaa ggaaacattt tgcgttactt acagaaatga aaaaacgctt caggaaacaa
      241 tgatcagtta tacccactgc tatattgaat tgcctttttt ggttgttctc agtctcggtc
      301 agaaagcatt aattagttct ttaagccgta ttttttcaac ttataccgca acatgcgttc
      361 cgacatacct agaatctcgg cggcttttgt ctggttatcc tgagcctttg acatcgattc
      421 agcaatcaat tttttttcca gctcttctac ggattcccgc aaaaggctgg cagacttctt
      481 gcccgaatcg cgagaagggg atgccgattt aaagggcaag tcctccgttg taatgtattc
      541 atcacgtgca ataaccactg ccctctccag aatatttacc aactcgcgca cgtttcccgg
      601 ataatcatat ttcaaaagtg agtcccgcgc ttcggcggtc aaaccttgaa tatttttccc
      661 gttttctttg gcaaatttat caatgaaatg atcgattaaa ataggcagat catcttttct
      721 ctctcttaac gggggtagtg acatggtcac aacttttaat ctatagaaaa gatcctcacg
      781 aaaagttcct tctttgacct gaactcctaa gtcgcggttt gtcgcgctga tgacccggac
      841 atcaacatgg atatcaacat tactgcctaa acgctgaaat tcgcgttcct gtaaaaaccg
      901 cagcagttta acctgtaccg tcgggggtaa ctctcctatt tcgtcgagaa aaagcgtgcc
      961 gccatcggct tcttcaaatc ggccgatccg gcggaaagtt gcgccggtaa aagcgccctt
     1021 ttcatgaccg aacaattcgc tctccaataa attttcatgc agggcgacac aattaactac
     1081 gatgatcggt ttatgagccc gggtacttaa ttggtgaatc agccgcgcca acagctcctt
     1141 tcccgttccg ctttcccctt gaatcagaac agatgctttg ctcgcggcta cacgacccgc
     1201 catattaatc acttccgcca tacgcgggct tttgtaaact attttatcgg cagtgacacc
     1261 ttttttgcct aattcttccc ttaaaagctc attttcccgg atgagttgtc ttctttccgc
     1321 cacccggttg accagtatta ataattcgtc gagttccacc ggcttggtga tgtaatcaat
     1381 tgctcctact tttatggctt ccactgccgt ttctattgtg ccgtaagcgg taatgataac
     1441 cacatctatt tccggattga tgcgttttat ttctttcagt acctccagtc catccatacc
     1501 gggcattttg taatccagga gaattaagtc aaaatgattg cttaaaattt tttttattgc
     1561 aatttcacca ttttccgctt ccgctacagt gctgccttcg gaaagcaaaa aatcgcgcag
     1621 catttcgcgc tgggattggc catcttcgac gataagaata ctcaatttat gcattgattg
     1681 ttatccctta atcttttaac ggctttacgc tgagtctttc acgggcaaaa caacttcaaa
     1741 tgttgttcct tcgccctccc ggctgataac ccttatatct cctccatggc cgcgtattat
     1801 ttcactggct aaaggtatac ccaagcctaa tcctttttct ttggttgtgt attccggatt
     1861 aaaaattctt tcgatttctt cagccgtcat gccgcagccc gtatcctcaa tactaacaac
     1921 tatatatttt ttcccttctt tatccactgt aattataatt ttaccttccg caggcataga
     1981 ctccatggca tttttaatta aatttagaaa tgcctgttgg agcttattta tgtccattgg
     2041 aattattgcc ggagtatgcc tccattttgt ttcaataaca acgttgcgtg ccgaggcttc
     2101 ttcccgaatc agactgacaa ttttttgtaa aacttctgta acagaaaaat tatgcaactc
     2161 caatctgcga cttttggaaa aagcgagaaa ctcttcaatt attccgttga gacgtttgat
     2221 ctcgtctctg atgacactgg aaagattctg aaaatcatcc gccctattgg aatccagtgg
     2281 aacaaaatcc cttttcaatc tttgtgtggc catgctgatg gcgtttaatg gattacgaat
     2341 ttcatgcgcc acaccggctg ctagctgtcc gagagaggaa aggcgttccg ccttttccaa
     2401 tcgccgttcc atttcaacga tgccggccaa gtgacggttc tggtcatgat aaagcagcca
     2461 catagataat aaggtaatga tgacaacaaa aaataaaaaa ataaaaatac tttggcggtt
     2521 ttcggcaatg attttatcca tcgatccccg gtcgagaccg attctcacta taccgacaac
     2581 ttttttatcc aaagataatg gagcggccaa atccaaaatt ttgtcttctt cgtaattcaa
     2641 cttcctgctg gcaattttta ttttgcccgt taatattccc tcaaaatcga aatcactgtt
     2701 ttttaaacct ggcatcgggc ggccgacact gctgagcagt ttttcctgat cattg
//
LOCUS       DASVIM010000154         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1512790, whole genome shotgun sequence.
ACCESSION   DASVIM010000154 DASVIM010000000
VERSION     DASVIM010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512790"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VF305_03100"
     CDS             complement(<1..308)
                     /locus_tag="VF305_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416154.1"
                     /translation="MRNSIYLLLLVLVFFVAGCASTQDLKAMRSELNQKMEGKLAAMD
                     AELAAVKKNAANLESMRKGQANTAADIADLRDNIQQLRGQVEALKKDFALGEKRGEEY
                     "
     gene            complement(333..854)
                     /gene="pal"
                     /locus_tag="VF305_03105"
     CDS             complement(333..854)
                     /gene="pal"
                     /locus_tag="VF305_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX7416155.1"
                     /translation="MKKNLILIGIFAVLVFGLAIFTGCAEKKTVVTDGSAQEQKVAAA
                     QTADTTKEQPAKVQAAETAVAVNDINFDFDKSNIRPDAREILKTDADVFLKNSASNIV
                     IEGHCDERGTTEYNMALGQRRAQETKKYLVNLGIKESRMKTISYGEERPLDPGHNEDA
                     WARNRRAHFAVTP"
     gene            complement(1099..2424)
                     /gene="tolB"
                     /locus_tag="VF305_03110"
     CDS             complement(1099..2424)
                     /gene="tolB"
                     /locus_tag="VF305_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX7416156.1"
                     /translation="MKKINPVVALLLYFCASLIFSSNVIAKVYIDIDSPTFQQFPIAI
                     CDFGNSAASAAISTDIGITFADEIKKYLAMTGIFNILNKKSFLEEKNPGNTAATENIH
                     FSDWATIGADYLLRGNIIQNNKEIIVECRLFDVTRGEILFNKRYDPKINELKALSRTI
                     AADILFTLTGDEGEFNTKIAFVSKKGSKSDIYAISYDGSELKSITNHQTIVVAPRWSP
                     DGKFLAFTSYKDGYPEVYIRNLKTGTEKKVASSEGLNLCGSFSPDGKKLLLTMSKEDN
                     EELYVLEISTLKLKRLTNNYSIDVSPTWSPDGQRIAFVSNRAGSPQIYIMDADGNNTK
                     RITYEGNYNTSPSWSPRGGRIAYEGLINGNYQIFSVDEEGNNLLQLTFDAARNESPSW
                     SPSGRQIVYSSKTNYKSRICIINANGLNTRVLEENGNDLVMPAWSPRFK"
BASE COUNT          673 a          531 c          515 g          814 t
ORIGIN      
        1 tgatattctt cccccctttt ttcgccgaga gcaaaatctt ttttcagcgc ttctacttgg
       61 ccacgtagtt gctggatatt atcccgcaaa tctgcaatat ccgccgctgt gttggcttgt
      121 cccttgcgca tcgattccag atttgcggcg ttctttttta cggcggctaa ttcggcatcc
      181 atagcggcca gttttccttc cattttctgg tttaattccg aacgcattgc cttcaaatcc
      241 tgagtagaag cgcaaccggc aacaaaaaac accaatacca gtaaaaggag ataaatagaa
      301 tttctcaatt ttttcaccta attaaattac tattatgggg taacagcaaa atgggcgcgt
      361 ctgttcctgg cccaagcgtc ttcgttgtgg cccggatcta agggacgttc ttcgccgtag
      421 ctaattgttt tcattcttga ttctttaatg cccagattga ctagatattt ctttgtttcc
      481 tgagcgcgtc tttgtcccag agccatgttg tattcagtag ttcctctttc atcgcaatgt
      541 ccttcgataa caatgttgga ggcgctattt tttaggaata catcagcatc ggtcttaaga
      601 atttcacggg catcagggcg gatgttggat ttgtcaaaat caaaattaat gtcgttaaca
      661 gcaactgctg tctctgcagc ctgcaccttg gccggctgtt ccttagtagt gtccgctgtt
      721 tgcgcagctg caaccttctg ctcctgagca gagccatctg ttactactgt tttcttttcg
      781 gcgcagccgg taaaaattgc cagaccaaat acaagtacag caaaaattcc tattaagatt
      841 aagttctttt tcattatcat tgttctcctt tcttaatgcg aaaataaagt tgtcataact
      901 aaaaaactgc aaaagttaaa cacaattgac gtattgccgt caataaaaaa ttataccaaa
      961 atcgggacag tttttaacgc gattccctat agttgccttg cttttcacgt tcctgccgaa
     1021 ttcaggtttt atcatagtga tctctttata cggcaaagaa gaaaaaataa caacccccaa
     1081 aagttgggaa tggtgaaatt atttaaatcg gggcgaccag gccggcatta ctaaatcatt
     1141 gccgttttcc tctagcaccc ttgtatttaa accgttagca tttataatgc aaattctgct
     1201 tttataattt gttttggaac tataaacaat ttgcctgccg gaaggtgacc aggagggtga
     1261 ttcgttcctc gcggcatcga aagttaattg taaaagatta ttaccttctt catccacgga
     1321 aaaaatttga taattgccgt tgatcagccc ttcgtaagca atccggccac cgcgcggcga
     1381 ccacgaaggg gaagtattat agttcccttc ataagttatc ctttttgtat tatttccatc
     1441 agcatccatt atataaattt gcggcgagcc ggcgcgattg gaaacaaaag ctattctttg
     1501 gccatccggc gaccaagtag gagaaacatc aatagagtag ttgttggtca ggcgtttcag
     1561 ctttaaggtg ctaatttcca ggacatataa ttcttcgttg tcttccttac tcatagttaa
     1621 caataatttt ttgccatccg gtgagaaaga gccgcacaaa ttaagccctt cagaagaagc
     1681 cacttttttt tccgtaccgg tttttaaatt ccgaatataa acttccggat agccatcttt
     1741 ataagatgta aaggccagaa attttccgtc gggtgaccag cgcggcgcaa ctacaatggt
     1801 ttggtgattg gtaatgcttt tcaactcaga accatcgtag ctgatagcgt aaatgtcaga
     1861 ttttgagcct ttcttggata caaaagcaat tttggtgtta aattcacctt catcgccggt
     1921 aagtgtaaat aagatatcgg cagctatagt tcgcgaaagc gcttttagtt cgttgatttt
     1981 aggatcgtat cttttgttga agagaatttc tccacgggtg acatcgaaga ggcggcactc
     2041 gacaattatc tctttgttgt tttggatgat gttaccccgg agcaaatagt cggcgccgat
     2101 tgttgcccaa tcggagaaat gaatgttttc tgttgccgct gtattgccag ggtttttttc
     2161 ttctagaaag ctttttttgt ttaaaatatt gaaaatcccg gtcattgcta gatatttttt
     2221 gatttcatcg gcaaaagtaa tgcctatatc agttgatatc gcagcgcttg ctgctgaatt
     2281 accgaaatcg caaatcgcga tagggaattg ctggaaggta ggagaatcta tgtcaatata
     2341 aactttggct atgacattgc tgctgaatat cagagatgcg cagaagtata atagcaaagc
     2401 taccactggg tttattttct tcatggaaat gtttttacct ttcttgttct ttagcgcaat
     2461 tctgagggat ggaagcgtat acctatttca atataattgt ccatcattcc ctctggaaat
     2521 ggaggaaacg gca
//
LOCUS       DASVIM010000155         8100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_745211, whole genome shotgun sequence.
ACCESSION   DASVIM010000155 DASVIM010000000
VERSION     DASVIM010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745211"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /gene="truB"
                     /locus_tag="VF305_03115"
     CDS             complement(<1..626)
                     /gene="truB"
                     /locus_tag="VF305_03115"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX7416157.1"
                     /translation="MNGIVIIDKPAGKTSHDIVSEVKKIMDAKKAGHTGTLDPMATGV
                     LPICLNEATKLAQFLSGENKEYLATMLLGVKTDTLDTEGEVTGQSDKFVRDEEIKAAL
                     KQMVGKIRQVPPAYSAVKYCGKPLYKYARKGIFLETAAREVEIYSIVVEDISFPRVTF
                     QIVCSKGTYIRTLCSDVGDLLGCGACLCGLRRLRSGLFSADMAVSLEN"
     gene            complement(630..1583)
                     /locus_tag="VF305_03120"
     CDS             complement(630..1583)
                     /locus_tag="VF305_03120"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX7416158.1"
                     /translation="MSNKIIAAINEGQSFLITAHVRLDGDALGSELALYLMLKDMGKK
                     AIVYNQDDTPEHYRFLPGAQNIVHELGDIGQYDVGVVLDCSNLERVGEKSAQIGKIKT
                     LINIDHHISNGGFCLLSMLDGQASSTGELLYRLMREMRCNMTKDICTNLYAAIITDTG
                     GFRYSSTHRDTLLAAGDLVGNGANPQWISENIYENDSPARLKLLAKVLKTMSLDLENK
                     VGSLFVTQEALLKTGASLDHTEGFIDIPRTVKGIDVSVLYTQLGNKYFKVSLRSKGQV
                     NVEKVARKFGGGGHINAASCRIEGDIEEIKILILEAIKEIK"
     gene            complement(1576..1935)
                     /gene="rbfA"
                     /locus_tag="VF305_03125"
     CDS             complement(1576..1935)
                     /gene="rbfA"
                     /locus_tag="VF305_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX7416159.1"
                     /translation="MKFKRADRVAELIMAEMADIIFKEVKDPRLHGVTITAVKVTDDL
                     RNAKIFFVEMGKDECSETIKSGLAKATGFVRRELAKRLQLRLVPEINFIHDQSFGYGS
                     RIDKLLADIAKQEENNV"
     gene            complement(1925..2263)
                     /locus_tag="VF305_03130"
     CDS             complement(1925..2263)
                     /locus_tag="VF305_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX7416160.1"
                     /translation="MVVGYGIINLRIPESGSLKEKRSVLSKILKRTQNEFNVSIAETG
                     NLDSCKFAEIGFAFVGNDSHYINGKVDHLLRFVDNLRAAEILNSKIEIMVVSSFLEAA
                     NYETGKYDEV"
     gene            complement(2309..4918)
                     /gene="infB"
                     /locus_tag="VF305_03135"
     CDS             complement(2309..4918)
                     /gene="infB"
                     /locus_tag="VF305_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX7416161.1"
                     /translation="MPKKRVYELAKELGLENKELIAHLEKIGITVKAASSSLEDSEVE
                     RVKSELLADEPRQIVEERIKSTVIRRRAVRTPVEAPVEEAPEEKADQPEEDLAGRPTK
                     VSSTQPSAKVGKVKKEELPELPLAGAEAASAHPKKKIPKSEADAATPAIKGKPAAETI
                     GEEKFKQPSIVPSRPPIISKHKITPPETRQGIPPKAPLKPGEKMIIPPAEKPSKKEFE
                     KPRKKGKGPVEVFIEEEKEVPRRKVLEKKIEKKLKKQDEEKEVIFTKWRDEKKVAPTK
                     MKKTEITVPKAIKRRIKVGETIAVGDLAKRMGVKVSEVINKLMSMSVMASINQSIDYD
                     IATLVANEFSFQVEKAEMEFDQSLLKTPTAPENLKPRAPVVTIMGHVDHGKTSLLDVI
                     RQSNVIAGEAGGITQAIGAYHVHINGRDIVFLDTPGHEAFTAMRARGAQVTDIVVLVV
                     AADDGVMGQTIEAINHSKVAGVPIIVAINKIDKPGADPEKIKQSLTEYDLLSEQWGGE
                     TIFCEVSAKKKKGIEELLEMILLQADVMELKADPDRPARGIIIEAKLDRGRGPVATVL
                     IQEGTLKEGDSFVSKTEFGRVRAMIDDQGRRVKEAGPSMPVEVIGFSNVPQTGAEFFC
                     VEDEKKAHDIADYWSRKKREKDLSASSKITLEQLYQKIKEGVKDFNAIIKADVQGSIE
                     AISDALNKLSTDDIKLKIIHSSTGAISETDVMLASAANAIIIGFNVRPDARVVEVAQQ
                     EGVEIKLYDIIYNIIADVRAAMEGLLEPEYKEVVQGRAEVRELFKVPKIGTIAGCYVT
                     DGKIIRNGNLKLVRDSVVVFDGKILSLKRFKDDAKEVLAGFECGISIDGFNDIHVGDV
                     LESYINEQLERKL"
     gene            complement(4956..6221)
                     /gene="nusA"
                     /locus_tag="VF305_03140"
     CDS             complement(4956..6221)
                     /gene="nusA"
                     /locus_tag="VF305_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX7416162.1"
                     /translation="MLPELKRLIEQMGKDRGIDKEIITEALEAAMLTAASKKLGQSVD
                     IEAHYNDEAGEIEVFQFKTVVDKVLDPGTQISKEEARKTLDEGAEPGDSLGIKIETSS
                     FGRIAVQMAKQIIIQRVKDAERDNIYEEYKDRKGELLNGFVQRFEGGSIIVNLGRAEG
                     IVPPAEQIHRETYKRGERIRSYIVDVKKNSKGSQIILSRTHPAFLRALFEVEVPEISE
                     GLIDIVSVAREPGKRGKIAVSAKDKDIDPVGACVGMRGSRVQSVVQELRGEKIDIIPY
                     SNDAAKYVSSALSPAKVNRVFVDDENRTMTVIVPDDQLSLAIGKNGQNVRLAVKLTGW
                     KIDVKSEAMAAVKDEEGQNDLTKITGVGPAMAEKLFNKGVKSIAMVAAAEPELLSSIP
                     GIGEKTARQWIEAAGKILDEEALNNKKAS"
     gene            complement(6302..6772)
                     /gene="rimP"
                     /locus_tag="VF305_03145"
     CDS             complement(6302..6772)
                     /gene="rimP"
                     /locus_tag="VF305_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX7416163.1"
                     /translation="MDYDVKEKIRQLAEPVIADEGMELIHVECIKMHTRWIIRLYLDK
                     ENGVTLGDCTAVSSQLGDIFDIHDLIKGAYTLEVSSPGLDRPISRDQDFIKHKNSRVK
                     IKTSVKIEGMGNFHGILLDYIEENGQKVVLIDISGKTYRIPRREIAKANLADAG"
     gene            complement(6906..6980)
                     /locus_tag="VF305_03150"
     tRNA            complement(6906..6980)
                     /locus_tag="VF305_03150"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6946..6948),aa:Gly,seq:ccc)
     gene            complement(7135..>8100)
                     /locus_tag="VF305_03155"
     CDS             complement(7135..>8100)
                     /locus_tag="VF305_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX7416164.1"
                     /translation="LNAIRNNSITDDDIALFNKRHDSQFTAPSGAFYLSLTSTNDLAD
                     GINEEQLAKLPGIIWKAQGMIDGEFGKEYLPTAIELKLKKSAQIMLLNNDTYGRWING
                     TIGKVVGFKKDDEGGEIITAKLDNGELVEISPYTWKIYRFFLKNDELRSEDVGSFTQY
                     PVRLAFAITIHKSQGKTFENVIIDVGRGTFAHGQMYVALSRCTSLTGIVLKQPLKKSH
                     ILMDWHIVKFLTRTQYDKAEQKCSHEDKLQIIHAAIKEKKNLDILYLKAKDEKSRRTI
                     RPLFVGEMEYSGHPFLGMDAYCLTRKEKRRFNVDRILEVCVSSNK"
BASE COUNT         2025 a         2004 c         1568 g         2503 t
ORIGIN      
        1 taattttcta aagaaacggc catatccgcc gaaaataatc cgctgcgcaa gcggcgcaga
       61 ccacataaac atgccccaca tcccaacaaa tcacctacgt ccgaacatag tgttctgatg
      121 taggttccct tagaacaaac aatctggaaa gtcacccggg gaaaagaaat atcttcaaca
      181 acaatgctat aaatttctac ttctctggct gctgtttcca aaaagattcc cttccgcgcg
      241 tatttataca aaggcttgcc acaatattta actgccgaat aggcgggagg cacctgtcga
      301 attttcccca ccatctgctt aagcgctgct tttatttctt cgtctcggac aaatttatcc
      361 gattggccgg taacctctcc ctctgtatct aatgtatccg tcttgacgcc cagcagcatt
      421 gttgccaggt actctttgtt ctcaccggat aaaaattgcg ccagctttgt cgcctcattt
      481 aagcatatgg gaagaactcc tgtggccatc ggatcgagcg ttccggtatg acctgctttt
      541 ttggcatcca taattttttt cacttcgctc acgatatcat gagaagtttt gccggcaggt
      601 ttatcaataa tcactatacc attcataaat cactttattt ccttgatagc ttcgagaata
      661 agtattttga tttcttcaat atccccttct atacgacaag acgccgcatt aatatgacca
      721 ccgccgccaa acttcctggc cactttttcc acgttgacct gacccttgga tcgtaagcta
      781 accttaaaat atttatttcc caattgagtg taaagaacag atacatcgat gcctttaaca
      841 gttctaggaa tatcgataaa gccttcggta tgatccaggg atgcacctgt cttaagcaaa
      901 gcttcctggg ttacaaataa agaacccacc ttattttcca aatccaaaga catagttttc
      961 agcactttgg ccagcaattt taatcttgcc ggagaatcat tttcataaat attttctgaa
     1021 atccattgag gattagctcc atttcccact aaatcaccag ctgccagcaa tgtatcccgg
     1081 tgcgtgctgg aatagcggaa accgcctgtg tccgtaataa ttgccgcata cagattagtg
     1141 caaatatctt ttgtcatatt acaacgcatt tcgcgcatca gacgataaag tagttccccc
     1201 gttgaactgg cctggccgtc cagcatcgat aacaggcaaa agccgccatt ggaaatatga
     1261 tgatcaatat taatcaaggt cttgatcttt cctatctgcg ccgatttttc ccccacacgt
     1321 tccaaattgc tgcaatcgag aacgacaccg acatcatatt gtccaatatc tcccaattcg
     1381 tgaactatat tttgcgcccc cggtaaaaaa cggtaatgtt caggtgtatc atcttgatta
     1441 taaacaatcg ccttctttcc catatctttt agcataagat aaagcgccag ttccgaacct
     1501 agtgcgtctc catccaatct tacatgggct gtaattaaga aactttgccc ctcattaatc
     1561 gccgcaataa ttttgttaga cattattttc ttcctgcttc gctatatctg ccaacaactt
     1621 atcaatgcgg ctaccatagc caaaagattg gtcatgaata aaatttattt ccggcaccaa
     1681 acgcagctgc agccttttgg ccaactcccg gcgcacaaaa ccggtcgctt tagccaaacc
     1741 tgatttgatt gtctcagaac attcgtcttt gcccatttcc acaaaaaaaa ttttggcatt
     1801 acgcagatca tcagtaacct tcactgccgt tatagtaacc ccgtgcaggc ggggatcctt
     1861 cacttccttg aatataatat cagccatttc ggccatgatt aattcggcca ccctgtccgc
     1921 tcttttaaac ttcatcgtat ttgcctgttt cgtaattggc ggcttccaaa aaactggaaa
     1981 caaccataat ttctattttg ctatttaata tttcggctgc acgcagatta tcgacaaatc
     2041 taagtaaatg atcaaccttt ccattaatat aatgcgaatc attaccaaca aacgcaaaac
     2101 cgatttcggc gaatttgcag ctatcaagat tacctgtttc cgcaatggag acattaaact
     2161 cattctgtgt ccgcttcaaa attttgctca aaacgcttct tttttctttc aaagaaccgc
     2221 tttccggaat tctcaaatta attattccgt agccgacaac cataaagctt cttccgacac
     2281 catacagatt aaaaagaaat ccccattgtt agagtttcct ttctaactgt tcatttatat
     2341 aagattcaag tacgtcacca acatgaatat cgttaaatcc atcaatgctg atgccgcatt
     2401 caaaaccggc gaggacttct ttggcatcgt ctttaaagcg ctttaatgag agaatttttc
     2461 catcgaaaac aaccacgctg tcgcggacaa gtttcagatt gccattgcgt attattttcc
     2521 catcggtcac atagcagccg gcaatggtac cgattttagg caccttgaaa agttcgcgga
     2581 cttccgcccg gccctgaacg acttctttat attccggctc caagagacct tccatggctg
     2641 cccgcacatc ggcaatgata ttataaataa tgtcataaag cttaatttct actccttctt
     2701 gctgggcaac ttcaaccaca cgcgcatcag gacggacatt aaatccgatg atgatagcat
     2761 tggcggcgga agcaagcata acatccgttt cgctgatggc gcctgtggaa ctatgaataa
     2821 ttttgagttt tatatcgtca gtcgaaagtt tattcaaagc gtcggaaatt gcctcaatcg
     2881 atccttgcac gtcggctttg ataatagcat tgaagtcttt gacgccttct tttatttttt
     2941 gataaagttg ttctaaagta attttagaag aagcggaaag gtctttttct cgtttttttc
     3001 ttgaccaata atcggcgatg tcgtgcgcct tcttttcatc ttcgacacag aaaaattccg
     3061 cccctgtttg aggaacattg gaaaaaccga taacctctac cggcatggac ggtccggctt
     3121 ccttaacgcg ccgtccctga tcatcaatca tcgcccgaac gcggccgaac tcagttttag
     3181 agacaaaaga atcgccttct tttagagtgc cttcttgaat taaaaccgtt gccacaggac
     3241 cgcgaccacg atccaatttc gcttcgatta tgataccacg agcagggcga tcgggatcag
     3301 ctttcaattc cataacatcg gcctgtaata gaatcatttc caaaagctct tcaatgcctt
     3361 ttttcttttt agcggaaact tcgcaaaaaa tagtttcgcc gccccattgt tcggataaaa
     3421 gatcgtattc ggtgagggat tgtttgattt tttcggggtc agcaccgggc ttgtcaattt
     3481 tattgatggc cacgataata ggcactccgg ccactttgga gtgattaatg gcttcaatgg
     3541 tttgtcccat aacgccgtcg tcggcagcca caaccagaac gactatatcg gttacctgcg
     3601 ctccccgcgc ccgcattgcc gtaaacgctt cgtggccggg cgtatccaga aaaactatat
     3661 cccggccatt tatatggaca tggtaggcgc cgatagcttg agtaattcct ccggcttctc
     3721 cggcaattac attggactgt ctgatgacgt ctaaaagaga ggttttacca tgatcgacat
     3781 gccccatgat agtaacaaca ggcgcccgcg gtttgagatt ttccggagcc gtcggcgttt
     3841 ttaacaagga ttggtcaaat tccatttccg ccttttccac ttggaaacta aattcattcg
     3901 ccacaagggt ggcaatatca taatctatgg attgattaat gctggccata acactcatag
     3961 acattaattt gttgattact tcgcttacct tgacgcccat tcttttagct aaatcgccga
     4021 cggcgattgt ttctccgacc ttaatgcgcc ttttaattgc cttagggaca gtaatttccg
     4081 tttttttcat tttggtggga gcgacttttt tctcgtcccg ccacttagta aaaattactt
     4141 ccttttcttc atcctgcttt tttagtttct tttctatttt tttctctaaa accttacggc
     4201 gcggaacttc tttttcctct tcgataaata cttctacagg acccttgccc tttttccttg
     4261 gtttttcaaa ttctttcttg ctaggttttt ctgccggagg aataatcatt ttctcgccgg
     4321 gttttagcgg cgctttcggc gggattcctt gtcttgtttc cggtggggtg attttgtgtt
     4381 tagaaataat aggcggcctg gaaggaacta tcgacggctg tttgaatttt tcctccccga
     4441 ttgtttccgc ggccggcttg ccctttattg ccggcgttgc cgcatctgct tcgcttttcg
     4501 gtattttttt cttgggatgg gcagaagctg cctcggctcc ggcaagagga agttcaggca
     4561 gctcttcttt tttaactttg ccaacttttg ccgacggctg agttgaagat acttttgttg
     4621 gcctacctgc caaatcttct tccggttgat ctgctttttc ctctggggct tcttcgactg
     4681 gcgcttctac gggagtgcgg acggcacgac gtcgaattac cgtggatttt attctttcct
     4741 ccacaatttg gcgaggttca tcggctagca attcgctttt tactctttcc acttcgctat
     4801 cttccaaaga actggaagct gccttgaccg taatgccaat tttttccaag tgggcgatca
     4861 gctctttatt ttccaatcca agttccttgg ccaattcata aactcgcttt ttcggcatgc
     4921 gcgaaactcc ctaaattaaa tttccagttc tgaaactatg aagccttttt attgtttagt
     4981 gcttcctcgt ctaaaatctt gcccgccgcc tcaatccatt gccgggctgt tttttcaccg
     5041 atgccgggaa ttgaagataa taattcgggt tcagctgctg caaccatggc aatgctcttg
     5101 acacctttat taaagagttt ctccgccata gccggcccca cccccgtaat ttttgttaaa
     5161 tcattttgtc cttcttcatc tttgaccgcg gccattgctt cgcttttcac atcgattttc
     5221 caaccggtca acttaactgc cagacggaca ttttgcccgt ttttaccaat ggccaaagag
     5281 agttgatcat cgggaacaat taccgtcatc gtccggtttt catcatcaac gaagacacga
     5341 ttaactttag ccggagacag ggcgctgctt acatattttg ccgcatcatt ggagtaagga
     5401 ataatatcaa ttttttcccc gcgcaattcc tgcaccacac tttgaacccg cgagcctctc
     5461 atgccgacac aggcgcctac cggatcaata tccttatcct ttgcactgac ggcaattttt
     5521 ccgcgcttcc ccggctcccg ggcaacgctg acaatatcaa taagcccttc ggaaatttcc
     5581 gggacttcca cttcaaaaag tgctcttaaa aaggccggat gcgtccggga caggattatt
     5641 tgcgatcctt tggaattctt ttttacatca acaatatagg agcggattct ttcaccacgt
     5701 ttataggttt cgcgatgaat ttgctctgcc ggaggcacaa tcccttccgc gcgtcccaga
     5761 ttgacaataa tgctgcctcc ttcaaatctt tggacaaagc cattgagtaa ttcgcctttg
     5821 cggtctttat attcttcgta aatattgtcg cgttccgcgt ctttaactct ttgaataatt
     5881 atttgcttgg ccatttgcac ggcaattcgg ccaaaggaac ttgtttcgat ttttattcct
     5941 aaactgtcgc cgggctcggc gccttcatcg agagtttttc tcgcttcttc tttggagatt
     6001 tgcgttccgg gatcaaggac tttatcgaca acagttttaa attgaaaaac ttcaatttct
     6061 ccggcttcat cgttgtaatg ggcttcgatg tccacgcttt gtcccaattt tttacttgca
     6121 gccgtaagca ttgccgcttc cagggcttcg gtaatgatct ctttatctat acctctgtcc
     6181 ttacccatct gttcgatcag acgtttaagt tctggcaaca tttacaaatt cctccctttt
     6241 cttcgctgag atactgcaga atttcagcct gtcggcatta attccttgct caatatttaa
     6301 tttatccggc atcggcaaga ttggctttgg caatctcccg ccggggaatc cggtaagttt
     6361 ttccggaaat gtcgatcaaa acaactttct ggccgttctc ttcgatgtaa tccaaaagga
     6421 tgccgtgaaa attccccatg ccctcaattt ttacgcttgt ttttattttc actctggagt
     6481 tcttatgttt tataaaatcc tgatcgcgcg atatcggcct gtctaaaccg ggagaggaaa
     6541 cttccagcgt gtaagcgcct ttaatcaagt catgaatgtc aaagatgtca cccaactgac
     6601 tgcttacggc tgtacaatca ccaagcgtta ctccattttc tttatccagg taaagccgga
     6661 tgatccagcg cgtatgcatt ttgatacatt cgacatggat caattccatt ccttcatccg
     6721 cgataaccgg ctcggccaat tgcctgattt tttcctttac atcataatcc ataatggctc
     6781 agaaaataaa aaagtgggca tgggcccact catcaataca acaataatcg atggttcggt
     6841 aatcagctaa cacactatag caggcaaagc aaggaaaaaa tgctgaaacc ggttaagtta
     6901 aaaaatggag cgggcgatgg ggctcgaacc cacgacgtcc agcttgggaa gctgacattc
     6961 taccactgaa ttacgcccgc tcatattaat ccaatttgtg gtcatcggga tactgcgacg
     7021 aagccaacaa aggtagcccg aatgaacgca atttggagtg gaatcttatt caacacgggt
     7081 attttgtcaa ggtgtttttg ctgaatgcgc aagcattagc agcaggggtt tttctcattt
     7141 atttgacgat acgcaaactt ctaatattcg atccacatta aagcggcgtt tttccttacg
     7201 cgtcaggcaa taggcatcca tccccaaaaa aggatgtccg ctgtattcca tctctccgac
     7261 aaacagcggg cggatcgtac ggcgcgattt ttcatctttt gcctttaagt agagaatatc
     7321 taaatttttc ttttctttaa tcgctgcatg aatgatctgc agcttatcct cgtgggagca
     7381 tttttgttct gctttatcgt attgcgtgcg tgtaagaaat tttacaatat gccaatccat
     7441 caaaatatga cttttcttca gcggctgttt cagaactatt ccagtaagcg atgtgcagcg
     7501 cgaaagcgcc acatacatct gtccgtgggc gaaagtgccg cgtcccacat caatgataac
     7561 attttcgaag gttttcccct ggcttttgtg gatagtgatg gcaaaggcca aacggacagg
     7621 atactgggtg aacgacccga catcttcgga acgaagctca tcatttttta aaaagaaacg
     7681 gtagattttc caggtatagg gactaatttc cacgagttcg ccgttatcca atttggcggt
     7741 aattatttct ccgccttcgt catctttttt gaatcccact actttgccga tggtgccgtt
     7801 aatccacctt ccataggtat cattgttcaa aagcattatt tgggcgcttt tttttagttt
     7861 caattcaatt gctgtcggca gatattcttt gccgaattcg ccatcaatca taccttgcgc
     7921 tttccatatt atgccgggaa gcttagccaa ttgttcctcg ttaatgccgt cggctaaatc
     7981 attagtgctt gtcaaagaca gataaaaagc gccggaaggg gcggtaaact gagaatcatg
     8041 gcgcttattg aacagcgcga tatcgtcatc cgtgatggag ttattgcgga tagcgttgag
//
LOCUS       DASVIM010000156         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1514898, whole genome shotgun sequence.
ACCESSION   DASVIM010000156 DASVIM010000000
VERSION     DASVIM010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514898"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VF305_03160"
     CDS             complement(<1..888)
                     /locus_tag="VF305_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX7416165.1"
                     /translation="MKFKKKILCIGAGYVGGPSMVMIAYKCPDCRVTVVDINPERIDA
                     WNSAELPVYEQGLYEIVKKIRGKNLFFAKDIENQIKENDIIFVSVNTPTKTFGADAGM
                     AADLQYWEKTARQILENSQSSKIVIEKSTLPVKTALAMEKILNSTKGKIRFDVLSNPE
                     FMAEGTAIEDLENPDRVLIGSHETRSGLKARQELVDIYSKWVPSEKIITSNIWSAELS
                     KLVANAFLAQRVSSINSISALCEKTDADISEVAKAVGMDSRVGSKFLQAGIGFGGSCF
                     KKDILNLVYLCSFYGLQEVA"
     gene            complement(912..1853)
                     /locus_tag="VF305_03165"
     CDS             complement(912..1853)
                     /locus_tag="VF305_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX7416166.1"
                     /translation="MKRILITGGAGFLGSHLCERLLTEGNDIACLDNFYTGSKDNVAH
                     LLGDPHFEIIRHDIINPVYLEVDEIYNLACPASPVHYQLNPIKTIKTNVIGVINILGI
                     AKRAKAKVLQASTSEIYGDPEVHPQNESYWGKVNPIGIRSCYDEGKRTAECLMMDYRR
                     QNDVNTKIARIFNTYGPRMAINDGRVVSNFIIQALQGNDITVYGDGAHTRSFCYVEDM
                     VDGLILMMNSTDDFYGPVNLGNPGEFTILQLAKLIIKLTKSKSKVQFQPLPADDPKQR
                     KPDITLAKKKLGWTPRIDLEEGLISTIEYFRDALKIS"
     gene            1955..2818
                     /gene="rsmI"
                     /locus_tag="VF305_03170"
     CDS             1955..2818
                     /gene="rsmI"
                     /locus_tag="VF305_03170"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX7416167.1"
                     /translation="MAENNSAKIGILYVVATPIGNLEDITLRAIRILKEVGLVAAEDT
                     RRTRKLLNAYKISTPLISLHEHNEKEKSTLIIAKIKSGMDVAYVSDAGTPCVSDPGHC
                     LVDFAGAENIRVIPIPGPSAVITALSASGFSADSFLFCGFMPARKNKRRKFMESLKDE
                     KRTIVFYESPMRFSATLQDMHDVLGNREIVVARELTKIFEEVKRGKILEFIAGKGQGR
                     TKGEFTIILKGEEYNPPPVADEKIEKRLLALWKNKKLSLRDAVVEVVSQTGLSKKKVY
                     DLAVKFCSENK"
     gene            complement(2949..>3485)
                     /locus_tag="VF305_03175"
     CDS             complement(2949..>3485)
                     /locus_tag="VF305_03175"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX7416168.1"
                     /translation="YDGRGLAAALALGADGISMGSRFMVVQESMVHENFKRLCLQATE
                     QDTLYGNVFDGMDGRVLKTKEAQTMMKRGFPLIEAIKGALLIKRLMNLSFLKFMGVSI
                     EMLRAEEGHPLMVLARQAVGNMKHLKALNEGDVEGGILFAGQCCGGITDILTTKELMG
                     RIISEAEATLAKLNTLKV"
BASE COUNT          952 a          753 c          757 g         1023 t
ORIGIN      
        1 ggctacttcc tgcaaaccat agaagctgca aagataaaca agatttaaga tatccttttt
       61 gaagcaggaa ccgccgaatc ctatcccggc ttggagaaat ttactgccca cacggctgtc
      121 cattcccacg gctttggcaa cttccgaaat atcggcatcg gttttttcac aaagagcgga
      181 aatcgagtta atggatgaaa cacgctgagc cagaaaagcg ttggcaacta atttggagag
      241 ctcggcgctc cagatattag aggtgattat tttttctgag ggaacccatt ttgagtaaat
      301 atctaccaat tcctgacggg cttttaagcc gcttcttgtt tcatgggaac cgataagcac
      361 gcggtcggga ttttccaaat cttcaatagc ggtgccttcg gccataaatt ccggattgga
      421 tagaacatca aatctaattt tgccctttgt tgaatttaga attttttcca tagccagggc
      481 ggttttcacg ggcagagtgc ttttttctat tacgattttc gatgattggg aattttccag
      541 aatttgccgg gctgtttttt cccagtattg taaatcggcg gccataccgg catcggcgcc
      601 gaatgttttc gtcggtgtat tgacgctaac aaaaatgatg tcgttttctt tgatttggtt
      661 ttcgatatct ttggcgaaaa acaaattttt gccgcggatt tttttgacta tttcatagag
      721 cccctgttca taaacgggaa gctctgcaga attccaggcg tcaatacgtt cagggttaat
      781 atccactact gtaacccggc aatcagggca tttataggca atcatgacca tagatggtcc
      841 gccgacgtaa cctgccccga tacagagaat tttcttctta aatttcattt attttgtatt
      901 gcctcagcta attatgagat ttttagagcg tcgcgaaaat attcgattgt gcttattaag
      961 ccttcttcta aatctatcct gggtgtccaa ccgagtttct ttttggcaag ggttatgtcc
     1021 ggcttacgtt gcttaggatc gtcagcaggc agaggctgga attgaacctt ggatttactt
     1081 ttggttaatt taatgattaa ctttgccaat tgcagaatag tgaattcgcc gggattgcct
     1141 aaatttaccg ggccgtaaaa atcgtcagtg gaattcatca tcaggattaa cccatcaacc
     1201 atatcttcaa cataacaaaa cgatctggta tgggcgccgt ctccataaac tgtaatatca
     1261 tttccctgca gggcttggat gataaaattg ctcaccacgc ggccgtcatt gatagccatg
     1321 cgcgggccgt aggtattaaa aatacgggct atttttgtat taacatcatt ttgccgccgg
     1381 tagtccatca tcaggcattc agcagttctt ttcccttcgt cgtaacaact gcgaattcca
     1441 atggggttaa ctttacccca gtaagattcg ttttgcggat gcacttcggg atcaccgtaa
     1501 atttccgatg tggaagcctg caacactttt gcttttgccc ttttagcgat acccaggata
     1561 tttattactc caatgacatt cgttttaatt gttttaatcg gatttaattg ataatgcacg
     1621 ggcgatgccg gacaggccag gttatatatt tcatcaactt ccagataaac ggggttgata
     1681 atgtcgtgac ggattatttc aaaatgagga tcgcccagaa gatgggcaac attgtccttg
     1741 ctgccggtat aaaagttgtc cagacaggca atatcgttgc cttcagtcaa caaccgttca
     1801 caaagatgtg aacctaaaaa tccggcgccg ccggtaatta atatacgttt cataatcaaa
     1861 atttgtcatt cctggtggta atttcgttta agctttgcta ttaaacatga aacaagctgc
     1921 agatgtcaac tataaaaata ttttataagg taatatggcc gaaaacaata gcgccaaaat
     1981 tggaatactc tacgttgttg ccactcctat tggaaatctc gaagatataa ctttgcgggc
     2041 aatccggata ttgaaagaag ttggccttgt tgccgccgaa gatacgcgcc gcaccagaaa
     2101 attacttaat gcttataaaa tatcaacgcc attaatcagc ctgcacgaac acaatgaaaa
     2161 agaaaaaagc acgctcatca ttgccaaaat caagtctgga atggatgtgg cttatgtgtc
     2221 cgatgccggc actccctgtg tgtccgatcc cggtcattgc ctggtagatt ttgctggggc
     2281 tgaaaatatt cgggtaattc ccattccggg gccatccgcg gtaattaccg ctttaagcgc
     2341 ttccgggttt tccgcagata gttttttatt ttgcggattt atgcccgcgc ggaaaaataa
     2401 aagacgaaaa tttatggaat ctctgaaaga tgagaaaaga acgattgttt tttatgaatc
     2461 acccatgagg ttttcagcca cacttcagga tatgcatgat gtgctgggta acagggaaat
     2521 agttgtggcg cgcgagttaa ccaagatatt tgaagaagtt aaacggggta aaatattgga
     2581 gtttattgcc gggaaagggc agggcagaac aaaaggggaa ttcacaatta ttttgaaagg
     2641 tgaagaatat aatcccccgc ctgtcgctga tgaaaaaata gaaaaaagac ttctggcact
     2701 ttggaaaaat aaaaaattat cattgcgcga cgcggtggtt gaagttgtga gtcaaacagg
     2761 tttatccaaa aaaaaggttt atgatcttgc cgttaagttt tgttccgaga ataaataaga
     2821 aaaaacgtaa ctaataaagt aggcgagatg tttgattcag tgcagggata ctgctttctc
     2881 tattttaatg gaaagtgcag gggaaaatcc ctgcactttc catgcttggt catctttctt
     2941 tttctgcgtt atactttcag tgtgtttagt ttagccaaag tcgcttccgc ctcactgata
     3001 atgcggccca taagttcttt tgtagtgagt atatcagtaa tgccgccgca gcactggccg
     3061 gcaaaaagga tgccgccttc cacatcgcct tcgttaagag ccttcaaatg cttcatattt
     3121 cccaccgcct gacgggcaag caccattagc ggatgtccct cttcagccct taacatttca
     3181 atgctgacac ccataaattt caaaaaggat agattcatca agcgtttgat cagaagcgct
     3241 cccttgatgg cttcgatgag cggaaacccc cgtttcatca ttgtttgggc ttcctttgtt
     3301 ttcagcacac ggccgtccat tccgtcaaac acattaccgt aaagcgtgtc ctgctccgtt
     3361 gcctggaggc acaaacgctt gaagttttcg tgcaccatgc tttcctggac taccataaag
     3421 cggctgccca tggaaatgcc gtccgctcct aaagccaatg ccgccgctaa tccccggccg
     3481 tcgta
//
LOCUS       DASVIM010000157         4488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_661718, whole genome shotgun sequence.
ACCESSION   DASVIM010000157 DASVIM010000000
VERSION     DASVIM010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661718"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            446..643
                     /locus_tag="VF305_03180"
     CDS             446..643
                     /locus_tag="VF305_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7416169.1"
                     /translation="MVDNIRKNRVKKLRETIPLSMAELARKAELAPQTIAKMEKGLST
                     RKNSELKVAKALQKPYEEVFP"
     gene            701..967
                     /locus_tag="VF305_03185"
     CDS             701..967
                     /locus_tag="VF305_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX7416170.1"
                     /translation="MKIGRYLVVFLLLMALLITFGNRGLVDNYLMSRRLAQLKSQSNE
                     MTSENNELKNKILLLRNDLTYMEAVARKELGMVKKGDIVYRLAK"
     gene            1015..2094
                     /gene="pilM"
                     /locus_tag="VF305_03190"
     CDS             1015..2094
                     /gene="pilM"
                     /locus_tag="VF305_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX7416171.1"
                     /translation="MADLKEFFGGTKKLVGLDIGSSSLKLAEVLITSHGHVLNRFLQI
                     PLPKGIIIEGVLADAKALSLKIKELFWNSGCRGKGIVTSLSGNSVIVKKVTFAQMDET
                     ELRDLIRDEAGKYLPFDNMDDVNYDFQILGDNDYNPNQMDVIIVAAKKDIVNSYLDAV
                     TTAGLNVTIMDVDSFALETMYEANYEFNNNEIVVIISIGASITNINVIKGGMSIFTRD
                     FTLGGNSITEGLQEKYRLTFEEAEKIKIEGIPNNDQDNLDLRNSILDFAEPICSEIER
                     SIDYFRSTFGGEYIKHVLLSGGSAKIHGLAANLSQKLNVETELINPFLKIGYNKKHVD
                     QKTLESIKPIAAVAIGLGLREIGDK"
     gene            2091..2639
                     /locus_tag="VF305_03195"
     CDS             2091..2639
                     /locus_tag="VF305_03195"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX7416172.1"
                     /translation="MIKINLLPYHEKKKKENVARQISITIGSFVIFILLLAWLQIWIS
                     SSVSALESQVKQAETLLVDLNKKIGDLEKFKNLKKELEQKLGVIKTLEENRLAAVKTL
                     DDLAALVPSKSIWLIKITQKGNGLVIEGTGKDNIAVADFMKTIEKFDPIKSVDLIASK
                     RIEISGITLQQFNFSCIMKKGF"
     gene            2642..3334
                     /gene="pilO"
                     /locus_tag="VF305_03200"
     CDS             2642..3334
                     /gene="pilO"
                     /locus_tag="VF305_03200"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX7416173.1"
                     /translation="MAFTFNDIKKLSPQAKALIIILIIFIIGYFDWFFFLSDAIENKT
                     SLDKKLEEMQGQIKEKEKIAIQINKYMADVESLKENYKIALQKLPDQREIPGLFHSVA
                     LAGKDAGIEFLLFEPKAAVPKTLDKQPSEQKQTGSSKAAPADGKKTPTPQQEPFYEEI
                     PVGVTVVGTFQNILYFFEKVAKLPRIVNISDISMGDRKDVKGRGYVITASCTIKTYMF
                     IDKKEKVSEKTK"
     gene            3331..3855
                     /locus_tag="VF305_03205"
     CDS             3331..3855
                     /locus_tag="VF305_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEX7416174.1"
                     /translation="MKKIKFTIASTFIIIFITAIFAFAAEVKNTLPSITPAPVNVVPA
                     TLQTQIQQPPPLAPTPENTYSYNPLGKPDPFRPFVEVDIVVKKDERKEIASIFPLQRI
                     ETENFRVVGIGGDNIRRVAVVEDATKKYYPLFTGTHIGLHNGKVIEIMHDRVIVEEYE
                     TKNAKRIILKLRKN"
     gene            3869..>4488
                     /locus_tag="VF305_03210"
     CDS             3869..>4488
                     /locus_tag="VF305_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416175.1"
                     /translation="MEKYLFKILPVFIIVLWLFPYAPIASLAAETTDESKATDNKAQA
                     VAAKAGYLENVLLEKFSGKERINLAVSQQPAINIESQTNGKLLVKLENMLAPENLRRP
                     LGEGELNNIISVTPSQQLIEGKQWIYLTIAIKELVPYAIKQEGQNVLIDFNVSSLVEK
                     TPKKVVAKVSAEPAKKSNDVAEIEASKAKPIQKETFKRYTNRLISL"
BASE COUNT         1541 a          719 c          895 g         1333 t
ORIGIN      
        1 tgcagcctct cgcttcgctc ttcataagcc tatttttcaa tatcaacgag aaaaaagtta
       61 ttactgcacc tttgtccagt tattacaaaa ccagataaat tgccaatatc ttgaaggaat
      121 ggatcgaagg cggtaaattt atacttggtg aaccccaaca aatattactc tccgtgcaaa
      181 tttaattata ctttattcat ttattaaagt ttttttgctt tgttcaatcc attagaatta
      241 tgcggcatta tttctaatat cacctaatca ttgacttttg aagttaacta tttatttgct
      301 aattatctat aattattaat aattatttat tctattaaca aagattaatt aatattaagc
      361 aattttaatc attattaaac atttttcttg acttttcttt aaaattagtg ttttttaaca
      421 atcaaatgag gtgatgcttt aatctatggt agataatatt aggaaaaatc gagtaaaaaa
      481 attgcgagag acaattcctc taagcatggc tgagctggcc aggaaggccg aattggcccc
      541 gcaaacaatc gccaaaatgg agaagggctt gtctacaaga aagaattcgg aattgaaagt
      601 agccaaggca ttacagaaac catacgaaga agtattccct tgaaaagcaa ccggtatatt
      661 atggatttat ccgctttgaa tgtaggagga ttgattccca atgaagatag gcaggtatct
      721 tgttgtcttt ttactgttaa tggcactttt gataacgttt ggcaataggg ggcttgttga
      781 taattattta atgagtaggc ggttggcgca attaaaatcg caaagtaatg agatgactag
      841 tgaaaacaat gaattgaaaa ataaaatact tttattgcgc aatgacttga cttacatgga
      901 agcggtagcc cgtaaagaat taggtatggt taaaaaaggg gatattgttt atcggctggc
      961 aaaatgatgg gaaagcatgt ttagcttagt taaaaacaac atgatggtgt tttaatggca
     1021 gacttaaaag aattttttgg gggtactaag aaactggttg gcttggatat cgggtcaagt
     1081 tctttaaaat tggctgaagt cctcattaca tctcatggtc atgttcttaa ccgcttctta
     1141 cagattccct tgcctaaagg cataattatc gaaggcgttt tagctgatgc caaagcgtta
     1201 tccttgaaaa tcaaagaact cttctggaat tccggttgta ggggcaaagg aatagttact
     1261 tctctttcgg gaaattcagt catagtaaaa aaggtaacat ttgcccaaat ggatgaaaca
     1321 gaattacgcg atttgatccg tgatgaagcg ggcaaatatt tgccctttga taatatggac
     1381 gatgttaatt atgattttca gattttgggt gacaatgatt acaatcccaa tcaaatggac
     1441 gtcattattg tggccgcaaa aaaagatata gtcaatagtt atcttgatgc ggtcacaacc
     1501 gccggcctca atgttactat tatggatgta gattcttttg cgctggaaac tatgtatgaa
     1561 gctaattatg aatttaataa taatgaaatt gtggtgatta tcagtatcgg agcgagcatt
     1621 accaatatca acgttattaa aggtggcatg tcgattttca cgagagattt tactttgggt
     1681 ggcaactcta ttactgaagg tttgcaagaa aaatataggt taacttttga ggaagcagaa
     1741 aaaataaaaa ttgaaggcat tcccaataat gatcaggata acttggatct aagaaacagc
     1801 attttggatt ttgccgaacc gatttgttca gaaattgaaa gatccattga ttatttccgc
     1861 tcaacttttg gcggtgaata tattaaacac gtacttctct ccggtggatc ggctaaaatt
     1921 catggattag ccgcaaattt atcacaaaag ctgaatgtgg aaacggaatt aatcaatccg
     1981 tttttaaaaa tcggctataa caaaaagcat gttgatcaaa agacgttaga aagcattaaa
     2041 ccgattgcgg cagttgccat agggttgggt ttgagggaaa taggtgacaa atgattaaga
     2101 ttaatctttt accttaccat gaaaagaaaa agaaggaaaa tgttgcccga cagatatcta
     2161 ttactatagg ttcgtttgtc atttttattc ttttgctcgc ttggctgcaa atttggataa
     2221 gttcttcagt tagcgcctta gaaagtcagg taaaacaggc ggaaactctg ctggttgact
     2281 taaataaaaa aataggagat ttagaaaaat ttaaaaatct taaaaaagaa ttagagcaaa
     2341 aactgggggt aattaaaacc ctggaagaaa atcgtttagc cgcagtaaag actttagatg
     2401 atctggctgc gcttgttccc tcaaaaagca tttggcttat taagataacc caaaaaggta
     2461 acggtctggt cattgaagga actggcaagg acaatatagc tgtagccgat tttatgaaaa
     2521 ctattgaaaa atttgatcca ataaagtcag tggatttgat agcgtcaaaa aggatagaga
     2581 tttccggcat tactctacag cagtttaatt tttcgtgtat catgaaaaaa ggattctaat
     2641 aatggccttc acatttaatg atataaaaaa attgtctcct caggccaagg cgctgattat
     2701 aattttgatt attttcatta ttggttattt cgattggttt ttctttttaa gtgatgccat
     2761 agaaaataaa acttctcttg ataaaaaact ggaagagatg caagggcaaa ttaaagaaaa
     2821 agagaaaatt gctatacaaa ttaataaata catggctgac gttgagtcat taaaagaaaa
     2881 ttacaaaata gctttacaaa aacttcccga ccagcgggaa atccccggcc ttttccattc
     2941 tgtggcattg gcaggtaaag atgcgggtat tgagtttttg ttatttgaac ctaaagctgc
     3001 ggtgcccaaa acgctggaca aacagccctc cgaacagaaa cagacaggca gctctaaggc
     3061 cgcaccggca gatggcaaaa aaactcctac cccacaacaa gagccatttt acgaggaaat
     3121 ccctgtggga gtaacggtgg tgggtacttt tcagaacatc ctttactttt ttgaaaaagt
     3181 ggccaaatta ccgcgaattg tcaacatttc cgatatttcc atgggggata ggaaggatgt
     3241 aaaaggacgg ggatatgtta tcactgcatc ttgtacgatt aagacgtaca tgtttattga
     3301 taaaaaagaa aaagtaagtg aaaaaacaaa atgaaaaaaa taaaatttac cattgccagc
     3361 acctttataa ttatttttat tacagcaatc tttgcttttg ctgctgaagt aaaaaatact
     3421 ttaccttcga ttactcctgc tcctgtaaat gtagtacctg caacattgca aacgcaaatt
     3481 caacagccac cgccacttgc gccaactcca gaaaatactt atagttataa tccattaggc
     3541 aaacccgatc ccttccggcc ttttgttgag gtagatatcg ttgtgaaaaa agatgaaaga
     3601 aaagaaatag cttctatttt tccgttgcaa aggatcgaaa ctgaaaactt cagagtggtg
     3661 ggcatcggcg gtgataatat tcgtcgcgtg gctgttgttg aggatgcaac aaaaaagtat
     3721 tatcccttat ttaccggaac acatattggt ttacataatg gtaaagtaat tgaaattatg
     3781 catgatcgtg tcattgtaga agaatatgaa acaaaaaatg cgaaaaggat aattttaaaa
     3841 ctgcgtaaaa attaaatgag gtaaaaccat ggaaaaatat ttattcaaaa tactccctgt
     3901 ttttataatt gttctttggc ttttcccgta tgctccgata gcttctttag ccgcggaaac
     3961 aacggacgaa agcaaagcaa cggataataa ggcccaggca gttgctgcca aagccggtta
     4021 tttggaaaat gttctattgg agaagttttc cggtaaagag aggattaacc ttgccgtatc
     4081 ccaacaaccg gcaattaata tagagagtca gactaatggc aaattgctgg ttaagttgga
     4141 aaatatgtta gcgcctgaaa atctgcgtcg ccccttagga gaaggtgaat taaacaatat
     4201 tataagcgtg acgccatcgc aacaattaat agaaggcaag caatggattt atttaactat
     4261 tgccattaaa gagcttgtcc cttatgcaat aaaacaagaa ggtcagaatg tgttgattga
     4321 ttttaatgtc tccagtttag tggaaaaaac tccgaaaaaa gtagtggcaa aagttagtgc
     4381 ggaaccggca aaaaaaagta atgatgttgc agaaattgaa gcaagtaagg caaaacctat
     4441 ccagaaggaa acattcaaaa gatatacaaa tcgattgata tctcttga
//
LOCUS       DASVIM010000158         7251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993 k141_64020,
            whole genome shotgun sequence.
ACCESSION   DASVIM010000158 DASVIM010000000
VERSION     DASVIM010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64020"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VF305_03215"
     CDS             <1..433
                     /locus_tag="VF305_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139198277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX7416176.1"
                     /translation="DEKDLRIDTFRAGGKGGQHVNKTDSAVRITHLPTGIVVQCQNER
                     SQHKNKAMAMKYLKSRLYERELQLKNEKISEENKLKKDIAWGSQIRSYVLHPYKMVKD
                     HRTNLENSNANKVLDGDIDEFIQAYLMYTTNHSSPAAGKDS"
     gene            550..2526
                     /locus_tag="VF305_03220"
     CDS             550..2526
                     /locus_tag="VF305_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7416177.1"
                     /translation="MKENITQTHFSEKAKEPVLMIRIKRYCVVYTIVLSLIFALPLFS
                     FALGKKPASTVDKENTIPTTKKADDSLKEESKDDGSKNGNNLPIVNPGELLSSPIKTK
                     QDQEREEKQDKMEKVLDLLKVADNYWEKEDVENTINTLDKAYALILDAGGDVVIARQK
                     DDLRLLISKRLLSVYSAKQTTTNGKASEIPLITNEAVEREIRSFQGVERDFFIAAYQR
                     SGMYRPVIIKELKKARIPEELSWLPFVESGFKIKALSRARALGLWQFIPSTGYKYGLS
                     RDEWVDERMDVEKSTQAAIAYLKELHDMFGDWLTVLAAYNCGEGKVLRVISLQHINYF
                     DRFWDLYNLLPRETSRYVPRFLATLHIIKNPQKYGFDLSNTEKPIPVETVKVNKIMKL
                     EEIASRIEIPDEKLYLINSELRYKMTPDREYDLKIPKESLAKFNLVANEIPESEKPRF
                     LLARTDFIRHRVRSGESLNSIASKYGVPASRIVSYNRLNPQKGLVAGKRLTIPIFKER
                     RYAKGKSRQKNNKIQITSAGRYKVRKGDTLQMIALRSGTTETQIKELNNLQKDTIRSG
                     QILKIFPKENNSNSEEKNDNNRGGTKLVQKSNINMLSASDLDKFGTNKYIVTKGDNLY
                     SISKKNNINVAKLKELNNLSGDGKIIPGQILVVK"
     gene            2721..3587
                     /gene="panC"
                     /locus_tag="VF305_03225"
     CDS             2721..3587
                     /gene="panC"
                     /locus_tag="VF305_03225"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX7416178.1"
                     /translation="MQKMKIIESINEMQSYSESRRLQGGKIAFVPTMGYFHEGHLSLM
                     KEAKKMADCVVVSIYVNPTQFGPQEDFSKYPRNFDRDLKMADSVNVDVIFYPSNDEMY
                     PANYQTYVHVEKITRNLCGISRPGHFRGVTTVCCKLFNIVKPHIAIFGKKDFQQFVAI
                     KSMITDLNLDLQIIGLATVREEGGLAMSSRNVYLKKDERSSALALVGALKLAQKLYAD
                     GERQSSVIIKEAKKLINTNSYTDIEYIKICDTATLDDVDEINDEAVIALAVKVGKTRL
                     IDNSVFGEELKA"
     gene            3614..3970
                     /gene="panD"
                     /locus_tag="VF305_03230"
     CDS             3614..3970
                     /gene="panD"
                     /locus_tag="VF305_03230"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX7416179.1"
                     /translation="MQRFMLKSKIHRAKVTDADLHYEGSISIDEKLMEEANMVTYEKV
                     EIYNVNNGARFSTYIIKGKRNSGTICLNGAAARKVAKGDLIIIATYVMVDDQEAKKWT
                     PKYVLVDSKNKIKKVH"
     gene            4156..5340
                     /gene="metK"
                     /locus_tag="VF305_03235"
     CDS             4156..5340
                     /gene="metK"
                     /locus_tag="VF305_03235"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX7416180.1"
                     /translation="MQITSESVKIGHPDIVSDSIAANIIAAILNEEHKICMDIHCMPH
                     CGLEVFLGKGLCVVGGEVSTRIYINIEKTVRQTVLDIGYCDSALGLNGSSMGILNAII
                     PQSPDINIGSRADLGKHKEIGAGDQGIIYGFACDETPELLPLPYVLVNRMMRAFENCG
                     NPIFAPDGKGQVTVDYDSNWRPKRLATVLMSNAIDYRQVPDGFRSSIEDQARQIALDC
                     LSDWTDDKTKFLFNPTGEWQAVNSCSAADSGVTGRKLVVQLYGGYPGAQLGGGAIVNK
                     TPEKVDCSAAFGSRHVAKNIVAAGLAKKCSVQLAYAIGIAQPISVYVNTFGTGIIPDE
                     KIASIINKIFDLTPKGMIDKFDLLNGDIFKKLPKTFFLDMNYLWEKTDKVDELKKALA
                     IK"
     gene            5431..6855
                     /gene="ahcY"
                     /locus_tag="VF305_03240"
     CDS             5431..6855
                     /gene="ahcY"
                     /locus_tag="VF305_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX7416181.1"
                     /translation="MAFLEINRALKYKVADLALASWGRKEMVMAENEMPGLMALRKKY
                     GRKKPLKGLKIMGSLHMTIQTAMLIETLKELGADLRWASCNIFSTQDHAAAAIAKSGS
                     AAVFAWKGETLEEYWWCTEQALTWPDGSGPDLVVDDGGDATLFIHHGVKIEKNPQLLK
                     DKIDNKEFKIIVSRLEQSFKKNPQRWQKVASRIRGVSEETTTGVHRLYQMQAAGELLF
                     PAINVNDSVTKSKFDNLYGCRESLADGIKRATDIMIAGKVVAICGYGDVGKGCAQSMR
                     GFGARVIITEIDPICALQAAMEGYEVKMLDEVAPIADIFVTATGCCDVITGKHMEMMK
                     DEAIVCNIGHFDSEIDMHYLETSKFCKKENIKPQVDKWKLKSGRSIIILAEGRLVNLG
                     CATGHPSFVMSNSFTNQCLAQIELASKKYKVGVYTLPKKLDEEVARLHLDRLNAKITK
                     LTKKQADYLNISVEGPFKPDYYRY"
     gene            complement(7050..>7251)
                     /locus_tag="VF305_03245"
     CDS             complement(7050..>7251)
                     /locus_tag="VF305_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX7416182.1"
                     /translation="SLRSERSGGVMQSIGIGIFVGFSYFVVHAFSMTLGRSGILPPFL
                     AAWAANIFFSAAATMLLYRART"
BASE COUNT         2448 a         1293 c         1580 g         1930 t
ORIGIN      
        1 tgatgaaaaa gatttacgca ttgatacttt tcgcgcgggt ggaaaaggtg gtcaacatgt
       61 taataaaacc gattcggcgg taagaattac ccacttaccc acgggaatag ttgtgcaatg
      121 tcagaacgaa cgttcccagc acaaaaataa ggcgatggcc atgaagtatt taaaatcaag
      181 gctctatgag agggaactgc aattaaagaa tgaaaagatt agtgaagaaa acaaattgaa
      241 gaaagatatt gcttggggaa gccaaatacg gtcttacgtt ctacatcctt ataaaatggt
      301 taaagatcac cggacgaatc ttgaaaatag caatgccaat aaagtcctag atggtgatat
      361 tgatgagttc attcaagctt atctcatgta tacgacaaac cattcttcac ctgcggctgg
      421 taaagatagt taatgggtaa attaatttat agcatgattg tttcagaagg ctggggatca
      481 gcaataaatt tgaggaaaaa ataaaaagta tgctatatta ataaatagtg aaagatattt
      541 atcaattaaa tgaaagaaaa tattactcag acacactttt cggaaaaagc taaggagccg
      601 gttttaatga taagaataaa aagatactgt gttgtttata cgattgtgct tagtttgata
      661 tttgctctcc ctttgtttag ttttgctctc ggtaaaaaac ctgcaagtac tgtcgataaa
      721 gaaaatacaa ttccaacgac aaaaaaagcg gatgattccc tgaaagaaga aagtaaagat
      781 gatggttcga agaatggaaa caacctgcca attgtaaatc caggtgagct gctttcttct
      841 cctattaaaa caaaacaaga tcaggaaaga gaagaaaagc aagataagat ggaaaaagtg
      901 ctggatttac tgaaagtcgc cgataattac tgggaaaagg aagatgtaga aaatacaata
      961 aatacattag ataaagctta tgcattaatt cttgatgctg gcggagatgt ggttatagcg
     1021 cgacaaaaag atgacctgcg attgttaata tctaaacgtc tgttgtccgt gtactcggcc
     1081 aagcaaacca ccacaaacgg taaggccagc gaaataccgt taataacgaa tgaggcagta
     1141 gaaagggaaa tccgctcttt tcaaggcgta gaacgtgatt tttttattgc ggcttatcag
     1201 cgctccggaa tgtaccgtcc agtcataatt aaagaattaa aaaaagcgag gattccggaa
     1261 gaactttcct ggcttccgtt tgtagaaagc ggtttcaaaa ttaaagctct atcccgggct
     1321 cgtgcgcttg gtttgtggca atttattcca tcaaccggtt ataaatacgg actaagccgc
     1381 gatgaatggg tagatgaacg catggatgtg gaaaaatcta cccaggcggc aattgcttat
     1441 ttaaaagaat tacacgacat gtttggagat tggctcaccg tattggctgc atataattgc
     1501 ggggagggaa aggtgctcag ggtcatatca ctgcaacaca ttaattactt tgatcgtttt
     1561 tgggatctat acaatctatt gccaagagaa acatcccgct atgttcctcg atttctggca
     1621 acattacata taatcaaaaa cccgcagaaa tatggatttg atttgagcaa tacggagaaa
     1681 ccaattccag tggagacggt caaagtcaat aaaataatga aacttgaaga gatcgcgtcg
     1741 agaattgaaa taccggatga aaaattgtac ttaattaatt cggaattgag atataaaatg
     1801 acaccggaca gagaatatga tttaaagatt cctaaagaat ctttagccaa gttcaattta
     1861 gttgctaatg aaatccccga aagtgaaaaa cctcggtttc tattagccag aactgatttt
     1921 atcagacacc gggtaagaag cggcgaatcg cttaattcta ttgccagcaa atatggagtt
     1981 cccgcttcca gaatagtatc ctataataga ttaaatcccc aaaaaggatt ggttgccgga
     2041 aaacgtttga cgattcctat atttaaagaa aggcgttatg caaaaggtaa atcaagacaa
     2101 aaaaataata agatacaaat tacatctgca ggacgatata aagttagaaa gggagacacg
     2161 ctacagatga tcgcgctgcg ttctggtact acagaaactc aaattaaaga gttaaacaac
     2221 ttgcaaaaag atacaatacg atcaggacaa atattaaaaa ttttcccaaa agaaaacaac
     2281 tctaattcag aagaaaagaa tgataacaat agaggaggaa caaagttggt tcagaaatcc
     2341 aacataaata tgttatcggc aagtgatctg gataagtttg gaacgaacaa atatattgtt
     2401 actaagggtg ataatttata cagcatctcc aagaaaaaca atatcaacgt tgctaagcta
     2461 aaggaattaa ataatctgtc cggcgatgga aaaattatcc ccggacagat tttggttgtt
     2521 aaatagataa atcaaaaagg acattcaggg gaaaaattag aaagctttga tgatattgtt
     2581 tgcccccaat tttatttcct tgacaaaacc tattaaatat tgttaatatt tcttcacgct
     2641 tggatccgca aggactggga cggaaggaaa aaataaagga tattataaag cctctcgtct
     2701 tgcgacgtga ggctttttta atgcaaaaaa tgaaaattat tgaatcaata aatgaaatgc
     2761 aatcttattc cgaaagccgc cggcttcaag ggggaaaaat agcatttgtt cccacaatgg
     2821 gttactttca tgaaggccat ctttctctca tgaaagaggc aaaaaagatg gccgattgcg
     2881 tcgtcgtcag tatttatgtg aatccaacac agtttggtcc ccaagaagat ttttccaaat
     2941 atcctcggaa ttttgatcgt gatttgaaaa tggctgatag tgttaatgtg gatgtaatct
     3001 tctatccatc aaatgacgaa atgtatcccg ctaattatca gacttatgtc catgtagaaa
     3061 aaataacccg gaatttatgc ggaatatcac gacctggtca ttttcgcggc gtcaccacag
     3121 tttgctgtaa acttttcaat atcgttaaac cgcacattgc cattttcggc aaaaaagact
     3181 ttcaacaatt tgtcgcgatt aaaagcatga ttacagacct caaccttgat cttcaaataa
     3241 taggtttagc cacggtacgc gaagaaggtg gcctggcgat gagttcgcgt aacgtctatc
     3301 tcaaaaaaga tgaaaggtca tccgccttgg ctttagtggg agctctcaag ttggcgcaaa
     3361 agctttatgc agacggggaa agacaatcgt ctgtaataat taaagaagca aaaaaactaa
     3421 taaatacaaa cagctataca gacattgaat acataaaaat atgtgatacg gccactctcg
     3481 atgatgttga tgagattaat gatgaggcag ttatcgcgtt agccgtaaaa gtagggaaga
     3541 cgcgacttat agataattcc gtttttggcg aggagttaaa agcgtaatat atttagcttc
     3601 aggagaaata actatgcaaa gatttatgtt aaaatcaaaa attcatcgcg ctaaggtaac
     3661 agatgccgac ttacattatg aaggcagtat ttccattgat gaaaaactga tggaagaagc
     3721 aaacatggtg acttatgaaa aagtcgaaat atacaatgtt aataatggcg caagattttc
     3781 aacttatatt ataaaaggaa agcgcaattc cggaacgatt tgtctcaatg gcgccgctgc
     3841 ccgcaaagtg gccaaaggag atttgatcat cattgcgaca tatgtcatgg ttgatgatca
     3901 ggaagcaaaa aaatggacgc caaaatatgt cttggtcgat agtaaaaata agataaaaaa
     3961 agtccactaa atgagcaaca atgctgaaaa ttattaagta tgatctggat atattctagg
     4021 gtatttatcc gtctttttca tttttaaaat caatctttca gaagggataa aaatattgga
     4081 ctccaatagg catgtgtgtt aattaccaac ataattttta tcctaaacat taaataaaca
     4141 taaggagatt caaacatgca gattacttct gagagtgtaa aaataggaca tcccgacata
     4201 gtttccgatt ctattgccgc aaacattatt gcagcaatac ttaacgaaga acataaaata
     4261 tgtatggata tacactgcat gccacactgt ggtctggaag tttttttagg caaaggactt
     4321 tgcgttgttg gcggtgaagt ttccacacga atttacataa atattgaaaa aacagtgcgc
     4381 caaacggtgc tggatattgg ttactgtgat tctgccctcg gccttaacgg cagttcaatg
     4441 ggtatactaa atgcaattat tccgcaatcg cctgatatca acataggaag cagagctgat
     4501 ttaggaaaac acaaagaaat aggagcaggg gatcaaggaa ttatttatgg ttttgcttgt
     4561 gatgagacac cggaactatt gcctctgcct tatgttctgg tgaatagaat gatgcgtgca
     4621 tttgaaaatt gcggtaatcc tatttttgcg ccggatggaa aagggcaggt aactgtggac
     4681 tatgatagca attggcggcc gaaacggtta gcgacagtac tcatgtctaa cgctattgat
     4741 tacaggcagg ttcctgatgg attccgttct tcaattgaag accaggcccg ccaaattgcc
     4801 ttggattgcc taagcgactg gaccgacgat aaaacaaaat tcctttttaa ccccaccggc
     4861 gaatggcagg cagtcaattc gtgttcggct gcagattcgg gagtaacggg acgcaagcta
     4921 gttgtgcagc tctatggtgg ttatccaggc gctcaactgg gtggtggtgc tattgtcaat
     4981 aaaacgccgg aaaaggttga ttgttcagca gctttcggtt cccgacatgt ggccaagaat
     5041 attgttgctg cggggctcgc gaaaaaatgt tcagtgcaac ttgcctatgc aatcggtatt
     5101 gcccagccaa tttccgttta cgtcaataca ttcggtacgg gaatcattcc ggatgagaaa
     5161 attgcctcaa ttataaataa aatatttgat ctaactccca agggtatgat tgataagttt
     5221 gacctgctca atggagatat atttaagaaa ctacctaaga cattcttttt ggacatgaat
     5281 tatctttggg aaaaaacaga taaggttgat gaacttaaaa aagcattggc aattaaataa
     5341 taataaagat ttgtttttac caatagacct caatccgctt ggtgtagtta tattgatctt
     5401 aagaaattgg caactgaact tggaggaaaa atggcttttc tagaaattaa tcgagcttta
     5461 aaatataaag tagcggatct tgcgctggca tcttggggaa gaaaagagat ggtgatggcg
     5521 gaaaatgaga tgcccgggct catggctttg cgtaaaaaat atggacgaaa gaagccccta
     5581 aagggattaa agataatggg tagtctgcat atgactattc agacggccat gctgatcgaa
     5641 acactcaagg aattaggcgc agacctgcgt tgggcttcat gcaatatttt ttccacccag
     5701 gatcatgcgg cggcagctat agccaaatca ggttcagcag ccgtgtttgc ctggaaaggt
     5761 gaaacgctgg aagaatactg gtggtgcaca gaacaggcct tgacctggcc ggacggttcc
     5821 ggacctgatc tggttgtgga tgatggcgga gatgccacat tgtttattca tcacggagtc
     5881 aagatcgaaa aaaatccgca attactgaaa gataaaatcg ataacaagga atttaaaatt
     5941 attgtttctc gtcttgaaca atcattcaag aaaaatccgc agcgctggca aaaagttgcc
     6001 tcccgaattc gcggagtatc ggaagaaact accactggtg ttcatcgtct ttatcaaatg
     6061 caggcggcag gagaactttt attcccggca ataaacgtca atgattctgt tacgaaatca
     6121 aaatttgata acctttatgg ttgccgtgaa tctcttgcag atggcatcaa aagagcaaca
     6181 gatattatga ttgccgggaa agtcgttgct atttgcggtt atggagatgt aggcaagggt
     6241 tgcgcccaat ccatgagggg ttttggagcc cgggtgatta ttacggaaat tgatcccatc
     6301 tgcgctctgc aggcagcaat ggaaggttat gaagttaaaa tgctggatga agtggcaccg
     6361 attgccgata tttttgtcac agccaccggc tgctgcgatg tcattaccgg caaacatatg
     6421 gaaatgatga aagatgaagc catcgtttgt aacattggac attttgatag tgaaatagat
     6481 atgcattatc tggaaaccag caaattctgt aaaaaagaaa atatcaaacc gcaggtagat
     6541 aagtggaagt taaaatcagg ccgatccata ataattcttg cggaaggccg actggtcaat
     6601 ttaggctgtg ccacaggaca tcccagtttc gtgatgagca atagttttac caatcaatgt
     6661 ctggcgcaaa ttgaattagc ctctaagaaa tataaagtcg gcgtttacac tctgcctaaa
     6721 aaattggatg aagaagtcgc ccgtttacac ttagatagat tgaatgcgaa gataaccaag
     6781 ttgacaaaga aacaagcgga ttatctgaac atatctgtcg aagggccgtt taaaccggat
     6841 tactacagat attaaaatca attctaaatc aaagcgctat ctttgttggc agcgccagcc
     6901 cggcgtatgc ttgggtcgtt atcggcttcc tcaacgtata acctgatacc ctaaagggca
     6961 cgcgaggtcg cgcgtgtata atacgcctcg tcgcctccgc ctagccgcct cgatatcacc
     7021 tttgatagaa ttggtataag ttgtaaagtt taagtgcgcg ctctataaag cagcattgtg
     7081 gcagcggcac tgaaaaatat atttgccgcc caagcagcta gaaacggtgg caatattcca
     7141 gagcggccca gagtcatact gaaggcatgg accacaaagt aagaaaatcc aacaaatata
     7201 ccgatgccga tactctgcat aacaccaccg cttctttccg agcgcagaga a
//
LOCUS       DASVIM010000159         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1259190, whole genome shotgun sequence.
ACCESSION   DASVIM010000159 DASVIM010000000
VERSION     DASVIM010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259190"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VF305_03250"
     CDS             <1..431
                     /locus_tag="VF305_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX7416183.1"
                     /translation="AWGMVAAILPALNNYYSFNGGLIIAFAFTFGVVFIRSAMSDILE
                     IQSDKLIGRETIPVLFGKERTVIVLKIISLMLFAFLLFSYPAGWSSSLSFFLLFCILY
                     IWICLQLCDKKSGFSGAVMEGLLETTYIIAGFCVLLWSVF"
     gene            473..1063
                     /locus_tag="VF305_03255"
     CDS             473..1063
                     /locus_tag="VF305_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416184.1"
                     /translation="MGRKLFVKCILMIFVLTMIACSGLRFSQLAPEAKDFHPQRVAIF
                     PADVVNYEEARGVVEKVVASVLIEKKWFTNVIDTENLNRQILANEELRKAIMEYLAKL
                     NTLDFSDSDLSRKIGELAKVDAFLLVSVDAWNYAVEKDKKVAKVSLTMRFYETSTGKV
                     MWKAGHNITESYILLKPDLPKVARDLVRDMVKEMPH"
     gene            1107..1328
                     /locus_tag="VF305_03260"
     CDS             1107..1328
                     /locus_tag="VF305_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX7416185.1"
                     /translation="MKEQVQKAIDLVRPGLQADGGDVELVDVSTDGVVKVKLTGACQG
                     CPMSQMTLKMGIEKIIKQQVPDIKEVISV"
     gene            1387..1566
                     /locus_tag="VF305_03265"
     CDS             1387..1566
                     /locus_tag="VF305_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7416186.1"
                     /translation="MAYVITDECIACGSCEDECPVEAISEGEDKYVIDAKLCTDCGAC
                     ADQCPVEAIVPGEEK"
     gene            1765..>2158
                     /gene="tmk"
                     /locus_tag="VF305_03270"
     CDS             1765..>2158
                     /gene="tmk"
                     /locus_tag="VF305_03270"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7416187.1"
                     /translation="MKKLITFEGIEGSGKSTQMRIVAQYLIEKNVPLLITQEPSGTDI
                     GRKIGGILFNRGHYYMCAETEAFLFCAARAQHIREMVMPALTQDRVVLCDRFSDATYA
                     YQGAGRGLDYEFIKFINEYSAMQLKPDLT"
BASE COUNT          606 a          373 c          513 g          666 t
ORIGIN      
        1 ctgcctgggg gatggtcgcg gccattttgc ccgccttgaa taattattat tcctttaatg
       61 gcggattaat cattgctttt gcttttactt ttggggttgt ttttattcgc tcagcgatgt
      121 cggatatttt ggaaattcaa agtgataaat taataggacg ggaaacgatt cccgtgctgt
      181 ttggcaaaga gcgcacagta atcgtcctca aaattatctc attgatgtta tttgcttttc
      241 ttcttttttc ttatccggca ggttggtctt catctttgag ttttttcttg ttgttctgta
      301 tattatatat atggatttgt ttacagcttt gtgataaaaa atcggggttt tccggggcag
      361 tcatggaagg ccttctggaa accacctata ttattgcagg tttttgtgtt ctcttgtgga
      421 gtgttttctg atatggagac gttctccaag gaagaaggag gttttaaaaa ttatgggaag
      481 aaaattattc gttaaatgta tcttaatgat ttttgtttta acaatgatcg cttgtagcgg
      541 attgcgtttt tcccaattag cgccggaggc aaaagatttt cacccgcaac gagtagctat
      601 tttcccggcg gatgtggtca attatgagga agccagggga gttgtggaaa aagttgtagc
      661 cagtgtgctt atagagaaaa aatggttcac gaatgtgatc gataccgaga atctcaatcg
      721 ccagattctg gctaatgaag aattgcgcaa agcaatcatg gaatatcttg ccaaattgaa
      781 tacacttgat ttttctgatt ccgacttgag caggaaaatt ggagagttag caaaagttga
      841 cgcgttcttg ctagtgtccg ttgatgcatg gaattatgcc gtcgaaaagg ataaaaaagt
      901 ggccaaagtt agtttgacta tgagattcta tgaaacttcc accggcaaag tgatgtggaa
      961 agccggccac aatataacgg aaagttatat actgctcaaa cctgatttgc ctaaagttgc
     1021 ccgcgatttg gttcgcgata tggttaaaga gatgccgcat taagcgggta aaaactatga
     1081 aatttaaacg gttaaggaga ggctagatga aagaacaggt gcaaaaagca attgatcttg
     1141 tcagacccgg actgcaagct gacggcggtg atgtagagct tgtagatgtt tctacggatg
     1201 gcgtcgtcaa agtaaaatta accggcgctt gccaaggttg ccccatgtct cagatgactt
     1261 taaaaatggg aatagaaaaa ataatcaaac agcaagtgcc tgatattaag gaagtcattt
     1321 ccgtttgagc tttccagtag attaagttat tggggaaaat tattgataga aggagattta
     1381 aggaacatgg catatgtaat tactgacgaa tgcatcgctt gcggttcttg tgaagacgaa
     1441 tgtcctgtgg aagcaatttc tgaaggcgaa gataagtatg ttatcgatgc taaactttgt
     1501 accgattgcg gagcttgtgc cgatcaatgt cccgtggagg caattgttcc cggggaagaa
     1561 aaataattta tttatggtaa acttacaaca gaggataaat ccgtcttctt caggaggatt
     1621 tatcctctgt ttcgcccctt aattgcaaac catccttact ttctatcaag tacgtctttt
     1681 tattttattt tagcgagaat cgaattgggg tttttgtgtt ttgtagagaa aattctccaa
     1741 gattaggtgc aggagactga accaatgaaa aaattaatta cttttgaagg gattgagggc
     1801 tcgggaaaat caactcaaat gaggattgtt gctcagtatt taatagagaa aaacgttcct
     1861 ctccttatta ctcaggagcc ctccggaacc gacattggcc gtaaaattgg aggtattctt
     1921 tttaaccgcg gacattatta catgtgtgcc gagacggaag cgtttctgtt ttgcgcggca
     1981 cgggctcagc atatccggga aatggtcatg cccgcgctaa cacaggatag agttgtcttg
     2041 tgcgaccgtt tttctgatgc aacttatgcc tatcaagggg ccgggcgagg cctcgactac
     2101 gaatttatca agttcatcaa cgaatattca gcgatgcaat taaagcccga tttaacct
//
LOCUS       DASVIM010000160         8978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_407931, whole genome shotgun sequence.
ACCESSION   DASVIM010000160 DASVIM010000000
VERSION     DASVIM010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8978
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407931"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..3867
                     /gene="mfd"
                     /locus_tag="VF305_03275"
     CDS             310..3867
                     /gene="mfd"
                     /locus_tag="VF305_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX7416188.1"
                     /translation="MRKIITKKNTEDIEPFQNLLRKINQGTKKVDVGGLTGAARPFLT
                     ASLFARLEQTLLVVCPEEKEAAAFAHDLALLLGEEAVFYYPSLDFLTIDMFALQKEEE
                     LARLEALTRLQTNTRTIVVTSVIALIQKVMPFAQFNHYLQIISIGGTLSRDEFSTHLM
                     SGGYKRVSLVEEKGEFSVRGNIIDVFPPTRGNPLRLEMAGDEIESIRLFDCSSQRSVG
                     STDAFILSPVSGVIINPPTLALAIRNIRRRSDDLSLPREIRNRLVDTLQNGLANSINP
                     IFLPLFYEAYDNNGGFSQNRLSSFFDYLPQNTLVILDNPLAINQAARNAELNIDKLVF
                     KTKNSSKFYLEKESAYIDLANTLAGMDRFAQVSLGGLKLDKDEDNGAVTITFETHQYL
                     YAEEHRAYEIKEDVLLRQTAEKIKLLLSEGMLIFFLCPTAEDLHRMNHLLLSYDLPVQ
                     IRTLQNSVIDCISQWEGNPQLILWEGKISTSFIIPAMKLVFLSEEEIFVKKAPRRRVR
                     PTREGYFLKSFGDLKEGDYAVHTDFGIGVYRGLKKITVGKIENDFLIIEYLDGDKLYI
                     PINSLEKIQRYLGPDGYVPKIDKMGGASWETVKERVKKSVREVAEELVAIYAAREVME
                     RKSFSPPDRIYEEFCSTFEFEETPDQAKAIEDVHLDMDDVKPMDRLICGDAGFGKTEV
                     AIRSAFRAVMDGKQVAVLVPTTILAEQHYQTFVSRLRDFPIRVEVFNRFKSTAEQKKI
                     VQDLRSQKIDIIVGTHRLIQKDVEFKELGLAIIDEEQRFGVAHKEKLKKMRTLVDVLT
                     LSATPIPRTLHLSLVGIRDLSIINTPPEDRVPIKTYVLEFDEDIIKAAVEKELARGGQ
                     VFFVHDRVRSIYSIAHLVQRLVPQAKIGVVHGQMKPAEIENAMAKFVRQECDVLVCTT
                     IIGSGLDIPSANTIIINRAEKFGLAQLYQIRGRVGRANQEAFAYLLLPRGVMLSREAM
                     KRLQVIKELSEPGSGFRIAYNDLEIRGGGNLLGISQSGHICAVGYELYTELMEKTVRE
                     IKGEKIGEEELLPEIQLGVSAFIPEEYVRDVHQRLVLYKRISLAANDEDIEEIKSELQ
                     DCYGTLPSSVVNLLQVISIRNRLKPLSAKKMGYDGKYLYLFFRDTSPVDPARIIALYR
                     KKIKELRFTPDYKLFVPAPALSEKEILTQADLLLKMLAQ"
     gene            3950..4900
                     /locus_tag="VF305_03280"
     CDS             3950..4900
                     /locus_tag="VF305_03280"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7416189.1"
                     /translation="MSSFRVLCVLILFFIVACDKIGIFSRPYVATVNGAKIYLDDYQL
                     RLNQKMSMLSKDISLNQPNYTKRFEEEILDAMITEKIMYLRAQELNISISATELEDKI
                     NEIKKDYGEDFSNLLAQENVRYEQWKEDLKKEMLMQKLIAVDVNARIRVSEDEAEDYF
                     NEHRNNYKAESRVRVAQIVVRDLAKAEEIAARLNAGGDFAKIAAEVSIGPEARRGGDL
                     GFITRQIMPEPLDETIFNLPVNKISPAVQSPYGFHIFKVLEIKPAGIRNFAEIKDNVI
                     ADIRAHKEDAAFISWLEALKIKAVIKKETSVLREKVNRRN"
     gene            4917..5891
                     /locus_tag="VF305_03285"
     CDS             4917..5891
                     /locus_tag="VF305_03285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7416190.1"
                     /translation="MEFYKRVIFIAWALMLMCMPAGAEVMDKIVAVVNDEVITLAEFN
                     EAFEPYRKNIEETYKGDNKEEVIKQTKEAFLKRLIDNILIEQEAKKADGGIIIKDEEV
                     MDVIKDMLAKKKSNMEDLKKNLVREGNSLESLKKDIRNQMMRTRLLRREIKSKVIVSD
                     EEIGEYYNKNRQDYEGKEAVRIKQILLLLPPKADKITREKLKNEAQKLRQRIMNGEPF
                     DLLAVNYSQGPAAAQGGDIGFIGKGAIIPEVEAVVFSLPVEQLSEVIESSVGFHIIKV
                     VDKRGAGLKPIAVVRVEIKEKIENEKLDKKFDEWIASVRAKSHIDIRL"
     gene            5910..8723
                     /gene="uvrA"
                     /locus_tag="VF305_03290"
     CDS             5910..8723
                     /gene="uvrA"
                     /locus_tag="VF305_03290"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7416191.1"
                     /translation="MGAIKIKGASQHNLKNISLDIPRDKLVVITGVSGSGKSSLAFDT
                     IYAEGQRRYVESLSTYARQFIGQMDKPDVESIEGLSPAIAIEQRTASHNPRSTVGTVT
                     EIYDYFRLLYAGIGVCHCYNCGREIKSQTIDSIVENVLSLPANTRFSIMAPLVRGKKG
                     EFQKELKKLQKEGFVRVRIDGEVRDLAEEIILTKTKRHDVDLVVDRLVLKEGVRKRLR
                     DSLEIAVNRSEGLVRIVPASGEEMLFSEKYACPDCTISIPELVPRMFSFNSPYGACPD
                     CNGLGSRMHFALDLVVPDAGLSLREGAIAPWAGRNSLYYHNVLDALSTHYKFDINTPF
                     NKLPEKIQKVILYGSGTESIKFYHDRPDRRYFAHRPFEGAIKQLERRWRDTASGAVRN
                     DLARYIDLSACDTCHGARLKKESLAVTVGGKNIYELCRMSIRECFNFFEQLTLTAQEK
                     IITERILKEIKSRLQFLLNVGMDYLSLERSTLSLSGGESQRIRLATQIGSGLMGVLYV
                     LDEPTVGLHQRDNLRLIDTLKKLRDMGNTVLVVEHDAEMMIACDHIVDMGPGAGANGG
                     EVVFQGSPEEILKSDESLTGRFLSGKESIALPSRRRSITGRQIILEGASENNLKNIDI
                     KIPVGVFTAVTGVSGSGKSTMVVETLYKVLARRLNQHRGAMGKIRRVTDLGDIERAIM
                     INQQPIGRTPRSNPVTYTGIFSFIRDLFANLPEARVRGYKPGRFSFNVKGGRCEACEG
                     NGLIKIEMHFLPDVYVTCDVCHGKRFNQDTLDIKYKDKSIAEVLDMTISQALIFFSNI
                     SPIKSHLQLLYDVGLGYIHLGQSATTLSGGEAQRIKLARELGKRTNSNTLYILDEPTI
                     GLHFADIQRLLDVLKRLVDMGNTIVIIEHNLDVIKCADYIIDLGPEGGPGGGQIIASG
                     TVEKVAGEEKSATGRFLRKILPA"
BASE COUNT         2721 a         1708 c         2142 g         2407 t
ORIGIN      
        1 cctagcgggc gaaaattcac cagaattgtt tttagagctt tttccacaga aatcatatct
       61 gctctccatt taaattatag ttgacagatt atcaaagaca aaatggttgt caacagataa
      121 gaagtgatta agttaaagaa tacttaagtg gacttgaatt aaatcttcat ttgagttaga
      181 aaaaaagatt gattttttaa cgaaaatttt acgggataaa ttttttcgat ttaattcccg
      241 acaaaagaag attaggggaa agccggcaat tgcttggctc ttcgccacaa aaagtttgga
      301 ttattatcag tgagaaaaat tatcacaaaa aaaaatacgg aagatattga gcctttccag
      361 aatctcctcc gaaaaattaa tcagggcacg aaaaaagttg atgttggcgg gcttaccgga
      421 gcagctcggc ccttcttaac agcttctctt tttgcccgcc tggagcaaac cctgcttgtt
      481 gtttgtccgg aagaaaaaga agcggcggct tttgcccacg atctggcctt gcttttaggg
      541 gaagaggctg tattttatta cccctctctt gatttcctga caattgacat gtttgctctc
      601 cagaaggagg aagaattggc ccgtctggaa gcgcttaccc gcctgcaaac aaacaccaga
      661 acaattgtcg ttacttccgt gattgctttg atacaaaagg tgatgccttt tgcgcagttc
      721 aatcattatc tgcaaataat ctctataggc ggaacactaa gcagggacga atttagtaca
      781 cacttgatgt ccggaggtta caaaagagtc agccttgtgg aagaaaaagg ggaattcagc
      841 gtccgtggta acattattga cgtatttcct cccaccagag gaaacccact acgtttggaa
      901 atggcgggcg atgaaatcga gtctatccga ctgtttgatt gttcctctca gcgttcggtc
      961 ggctcaacgg atgctttcat tctatcgccg gtaagcggag taatcatcaa tccgccaacg
     1021 ctggcgctgg ctatccgtaa tatcagacgg cgatccgatg atctctcgct tccccgcgag
     1081 attcgtaacc gtttggtaga tactttgcag aacggtttag ccaattccat caacccgata
     1141 tttttaccgt tgttttatga agcttacgat aataatggag gattttcgca aaatagattg
     1201 tccagttttt ttgattattt gccgcaaaat actttggtaa tattggataa tccgttagcc
     1261 ataaaccaag cagctaggaa tgcggaactt aatatcgaca aattggtgtt caaaacaaaa
     1321 aacagcagca aattttatct ggaaaaagaa agcgcttata ttgatttggc aaatacttta
     1381 gccggtatgg acaggtttgc gcaagtttct cttggaggcc tcaagctgga caaagacgaa
     1441 gataatgggg cagtgactat tacttttgaa actcatcaat atctgtatgc agaagaacat
     1501 cgagcctatg agattaagga agatgttctt ttacggcaga ctgcggaaaa gattaaattg
     1561 ttgctctccg aaggaatgtt gatttttttc ctttgtccaa ctgcagaaga cctgcaccgg
     1621 atgaatcatc tgcttttatc ttatgatttg ccggtgcaga ttagaacttt gcaaaattct
     1681 gtcatagact gcatcagtca gtgggaaggg aaccctcaac ttattttgtg ggaagggaaa
     1741 atttctacta gttttatcat tccagcaatg aagctggttt ttttaagcga agaagaaata
     1801 tttgtcaaaa aagcaccccg ccgccgcgtg cggccaacac gggaaggcta cttccttaag
     1861 tcctttggtg atttaaaaga gggtgattat gccgtccaca ccgattttgg tattggcgtt
     1921 tatcgtggac tgaaaaaaat taccgtgggt aaaatcgaaa acgattttct gattattgaa
     1981 tatttagacg gcgataaact ctacataccg attaattctc tggagaaaat tcaaaggtat
     2041 ctcggacctg atggttatgt gccgaaaatt gataagatgg gcggagcttc ctgggaaacg
     2101 gtaaaagaaa gagtaaaaaa atcagtgcgg gaggttgccg aggaactcgt tgccatttat
     2161 gccgcccgtg aagtgatgga aagaaaatcg ttctcgccgc cggatcggat ttacgaggaa
     2221 ttttgctcca cttttgaatt tgaagaaacg cctgatcagg ccaaagctat tgaagatgtt
     2281 catctggata tggatgatgt aaagccgatg gaccggttaa tatgcggcga cgccggtttc
     2341 ggcaaaacag aagttgctat tcgctcagca ttccgagccg ttatggacgg caaacaggtt
     2401 gccgttcttg tacccacaac aattttggcc gagcaacatt accagacgtt tgtaagcagg
     2461 cttcgtgatt tccccattcg tgtagaggtg ttcaatcgct ttaaaagtac ggccgaacaa
     2521 aagaaaattg tgcaagattt aagaagtcaa aagatagata ttatcgtagg aacacatcgc
     2581 ctgattcaaa aagatgttga atttaaagaa ctgggactgg caataattga tgaagaacag
     2641 cgtttcggcg tggcgcacaa ggaaaaactg aaaaaaatgc gcacacttgt cgatgtactg
     2701 actctttcgg caacaccaat tccgcgcacg cttcatcttt cccttgtagg tatccgggat
     2761 ttgtccatta taaacacacc gccggaggac cgtgtgccca taaaaaccta tgtgctggag
     2821 tttgacgagg atataattaa agccgcagtg gaaaaagagc tggcgcgggg ggggcaagta
     2881 ttttttgttc atgaccgggt gcgctccatt tattcgattg cgcatctggt gcaacgtctg
     2941 gtaccgcagg caaaaattgg ggttgtccat ggccaaatga aaccggcgga aattgaaaat
     3001 gctatggcca aatttgtccg gcaggaatgt gacgttcttg tctgcaccac aattatcggt
     3061 tccggtttgg atattcccag cgccaataca ataattatta atcgcgccga aaagttcgga
     3121 ttggcgcagc tctaccagat tcgtggccgt gtaggccggg ccaatcagga agcttttgcc
     3181 tacctgctat taccccgggg agtaatgctt tcccgcgaag ccatgaaaag actgcaagta
     3241 attaaagaat tatccgagcc aggttcggga tttaggatag cctacaacga tctggaaatc
     3301 cgcgggggag gaaatttatt aggcatttcc cagtccgggc atatttgtgc agttggttac
     3361 gaactttata ccgaattaat ggaaaaaacc gttcgggaaa ttaagggaga aaaaattggc
     3421 gaagaagaat tattgccgga aattcagttg ggagtttccg ctttcatccc tgaagaatat
     3481 gtgcgcgatg ttcatcaacg gttggtacta tacaaaagaa tatctttagc cgcgaatgat
     3541 gaagatattg aggagatcaa aagtgagctg caggattgtt acggaacttt gccatcgtcc
     3601 gtagtaaatt tgttgcaagt gattagcata agaaatcgcc tgaaaccgct ttcggccaaa
     3661 aaaatgggct acgatggcaa gtacttgtat ctttttttcc gggatacatc tcccgttgat
     3721 ccggcaagaa ttattgcgct ttatcgtaag aaaattaagg aattgcgttt tacgccggat
     3781 tacaaattat ttgtgcctgc gccggctctt tccgaaaaag aaatattgac ccaggccgat
     3841 ttgttattaa agatgcttgc ccaatagctg caagcaagat ataaagaaat gttatgtata
     3901 aaaatcgaac tagaattttt tctttaacta aaaaaagatg aggataataa tgagtagttt
     3961 ccgtgtttta tgtgtgctga tacttttttt tattgttgct tgcgataaaa ttggtatctt
     4021 ttcgaggcca tatgtggcga cggttaacgg cgccaagatt tacctcgatg attatcaact
     4081 gaggttaaac caaaaaatgt ctatgctctc caaagatatt tcattaaatc agcctaatta
     4141 tactaaacgc tttgaagaag aaatattaga cgcaatgatt acagaaaaaa tcatgtattt
     4201 acgtgctcaa gagcttaata tctccataag tgcgactgaa ttggaagata aaattaacga
     4261 aattaaaaag gattacggag aagatttttc caacttgctg gcgcaggaaa atgtaagata
     4321 tgaacaatgg aaagaggatt taaaaaaaga aatgcttatg caaaaactta ttgcggtaga
     4381 tgtcaatgcc cgtattaggg tatcggaaga tgaagccgaa gattatttta atgaacaccg
     4441 caataattac aaggcggaat cccgcgtaag ggtcgcccag atagttgttc gagatttagc
     4501 caaagccgaa gaaatagccg cacgattaaa cgcgggagga gattttgcta aaatagcggc
     4561 agaggtatct atcggccccg aagctcgccg cggaggagat ttggggttta tcacccgcca
     4621 gatcatgccg gaacctttag atgaaactat ttttaacttg ccggtcaata aaataagtcc
     4681 ggccgtgcaa agtccttatg gctttcatat tttcaaagtt ttggagatta aaccggcagg
     4741 aattagaaat tttgcggaaa tcaaagataa tgttattgcc gatattcggg cgcataagga
     4801 agatgcggct tttataagct ggcttgaagc attaaagata aaagcagtga tcaaaaagga
     4861 aacttccgta ttgagggaaa aagtaaatag gcgaaattaa aaaaggagga agcgctatgg
     4921 aattttacaa gagggttatt tttattgcct gggctttgat gcttatgtgc atgccggccg
     4981 gcgccgaggt tatggataaa attgttgcgg ttgtaaatga cgaagtaata acgcttgccg
     5041 aatttaatga ggcgtttgag ccttaccgta aaaatatcga agaaacctat aagggcgata
     5101 ataaagagga ggtcattaag cagaccaagg aggctttcct gaagcggctc attgataata
     5161 tattgattga acaggaagca aaaaaagcag acggtggtat tatcatcaaa gatgaagaag
     5221 tcatggatgt gatcaaggat atgctggcga aaaagaaatc caacatggaa gatttgaaaa
     5281 aaaatcttgt ccgtgaaggt aattctcttg aatctttgaa aaaagatata agaaatcaaa
     5341 tgatgcgtac gcggttattg cggcgggaaa ttaaatccaa agtaattgtc agcgatgagg
     5401 aaataggtga gtactataac aaaaacaggc aggactacga aggcaaagaa gcagtcagaa
     5461 ttaaacaaat tttattattg ttacctccca aggcggacaa gatcacgcgg gagaaattga
     5521 aaaatgaggc tcaaaaatta cgccagcgca tcatgaatgg agagcccttt gatttactgg
     5581 ctgtaaacta ttctcagggg ccggcggcag cccaaggtgg tgatattggt tttatcggaa
     5641 aaggggcaat tattccggaa gtagaagcag tggtatttag tcttcccgtc gagcagttaa
     5701 gcgaagtaat tgaatccagt gttggatttc atataattaa ggtagtggat aaaaggggcg
     5761 ccggattgaa accgatcgct gtggtgcggg tagaaataaa agaaaaaatt gaaaacgaaa
     5821 aattggataa gaaatttgac gaatggattg cttctgtccg ggcgaagtcg cacattgata
     5881 taagattgta aaaaataaca aagctgaata tgggcgctat aaaaataaaa ggtgcttccc
     5941 agcacaattt aaaaaatata agtttagaca tacctcgcga taagcttgtt gtcattaccg
     6001 gtgtcagtgg ttccggcaaa tcgtcgctgg cttttgacac gatctatgcc gaaggtcaaa
     6061 ggcgttatgt agagtcgctt tccacttatg cgcgccagtt tattggacaa atggataaac
     6121 cggatgtgga atccattgaa ggcctgtcgc ccgctatcgc tatcgagcaa cgcacagcca
     6181 gccacaatcc tcgttccact gtaggtacgg tcaccgaaat ttacgactac ttccgtctgc
     6241 tttatgccgg cattggtgtt tgccattgtt ataactgcgg gcgggaaatc aaatcgcaaa
     6301 caattgacag tatcgtagaa aatgttctat cgcttcccgc caatacacgc ttcagcatta
     6361 tggccccgct ggtgcgcgga aaaaaaggtg aattccagaa agaattaaaa aaactccaga
     6421 aagaaggctt tgtacgtgtc cggattgatg gagaagtcag agacctggcc gaagagatta
     6481 ttcttaccaa aacaaagcgc cacgatgttg atctcgttgt tgaccgcttg gtattaaagg
     6541 aaggcgtaag aaaaagactg cgtgattctc tggaaattgc cgtaaaccgg tcagaaggac
     6601 tggtgcgcat agttcccgct tccggcgaag aaatgctttt cagtgaaaaa tatgcctgcc
     6661 cggattgcac aataagcatc cccgaacttg tcccccgcat gttttccttc aacagtccct
     6721 atggagcttg tcctgactgc aatggtctgg gctcccgtat gcactttgcg ctggatctgg
     6781 ttgtgccgga tgccggattg tctttgcgcg aaggggcaat tgcgccctgg gcgggaagaa
     6841 attctcttta ctatcataat gttctggatg ctctctccac tcattataag tttgatatta
     6901 atactccgtt taataaatta ccggagaaaa ttcaaaaagt tatcctttat ggatcaggaa
     6961 cggagagcat taaattttat catgaccgtc ctgatagaag gtattttgct catcgcccct
     7021 ttgagggagc catcaaacaa ttggaacgcc gctggcggga tacagcttct ggcgcggtgc
     7081 gcaatgatct ggcgcgctac attgatttga gcgcgtgcga tacctgtcat ggcgctcgtt
     7141 taaaaaagga aagtcttgcc gtaactgtgg ggggaaaaaa tatttatgaa ctctgccgga
     7201 tgtccatccg ggaatgcttt aactttttcg aacaactgac actgacagca caagaaaaaa
     7261 tcataaccga aaggattctc aaggaaatta aaagccgtct gcaattcttg ctcaatgtgg
     7321 gcatggatta tttaagtctt gagcgatcaa cgctatcctt atcgggaggt gaaagccagc
     7381 gtatccggct ggctacgcaa atcggttccg ggttaatggg tgttttgtat gtgttggatg
     7441 agccgacggt cggactgcac cagagggata atctgcgtct gattgatact ttgaaaaaat
     7501 tgcgcgatat gggtaatacc gtgctggttg tggagcacga cgccgaaatg atgattgcct
     7561 gcgatcacat tgtagatatg gggccgggtg ccggagccaa cggcggggaa gtggtgtttc
     7621 aaggttctcc ggaagaaatt ttaaaaagcg atgaatcgct caccggtaga ttcttatccg
     7681 gcaaggaatc catagctttg ccgtcaagaa ggcgctcaat taccggccgg caaattattt
     7741 tagagggagc cagtgaaaac aatctcaaaa atattgatat caaaataccg gtaggagtat
     7801 ttacggctgt aaccggtgta tcgggttccg gtaaaagcac aatggtcgtt gaaactcttt
     7861 ataaagtgct ggcgcgccgc ttaaaccagc atcgcggggc aatgggaaaa atcagaagag
     7921 ttacggattt aggagatatt gaacgggcca tcatgattaa tcaacagccg ataggccgga
     7981 cgccgcgatc caatccggta acttacaccg gaatattttc ttttatccgc gacctatttg
     8041 ccaatcttcc cgaagcccgg gtgcgcggct ataagccggg acggttcagc ttcaacgtga
     8101 aaggcggccg ctgtgaggcg tgtgaaggga atgggttgat taaaattgag atgcattttc
     8161 tgccggatgt ttatgttact tgcgatgtct gccacggcaa acgttttaat caagatacac
     8221 tggatataaa atataaagat aagagcattg ccgaggtttt agacatgacg ataagccagg
     8281 cactaatctt tttttccaac atttcaccga ttaaatcgca cctgcaattg ctctacgatg
     8341 tcgggttggg ttatatccat ttggggcagt cggcaaccac tctttccggc ggcgaagcgc
     8401 aaaggataaa gctggcccgg gagttgggca agagaaccaa cagcaacact ctttatattc
     8461 tggatgaacc gaccattggg ttgcattttg cagatatcca aagattgctg gatgttttaa
     8521 aacgccttgt ggatatgggc aatactatcg tcataatcga acataatctg gatgtaatta
     8581 aatgtgccga ttatattatt gatcttgggc cggaaggcgg gccgggcggc ggtcaaatta
     8641 ttgcgtccgg tacagtggaa aaagtggccg gggaagaaaa atcagcgacc ggccgatttt
     8701 tgagaaaaat tcttcccgct taagagatca aattaataat aaggaatgcc cttcccacga
     8761 aaagccattt tggttcaggt cagaatttta aattggtaat aggaatttca tataattatt
     8821 aattaataat aacaataact taacatgatt atccgcaaga ttcaatattt ttacgtgaaa
     8881 gtaaaatgtt gatgatatat aataaaatgt aaaaacattc ttaataacgt ttccagggta
     8941 tggatgatag aatgacttca gtaccgctaa aacctgta
//
LOCUS       DASVIM010000161         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_321963, whole genome shotgun sequence.
ACCESSION   DASVIM010000161 DASVIM010000000
VERSION     DASVIM010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321963"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VF305_03295"
     CDS             complement(<1..442)
                     /locus_tag="VF305_03295"
                     /inference="COORDINATES: protein motif:HMM:NF025743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HEX7416192.1"
                     /translation="MSKPTPAEPVKLVFSVFATAGAQLSETINMLSALYGQPDFVSAL
                     MPFDYTNYYLPEMGENLIRRFLAMEKLIRPETLPDINLSTNEIENKLALDEKRQVNID
                     PGYLSKPHLVLATGKGYAHRPYLRDGIYADLTLIYQGKKFCSLPW"
     gene            complement(439..1428)
                     /gene="rfaE1"
                     /locus_tag="VF305_03300"
     CDS             complement(439..1428)
                     /gene="rfaE1"
                     /locus_tag="VF305_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX7416193.1"
                     /translation="MQKILTKKRALEIIANFSQTGVLVVGDVMVDHFIWGKVSRISPE
                     APVPVVDVQKDSILLGGCANVLNSIYSMGGKVYVAGVIGVDNIGKRLLMELRERKIET
                     NGIVEEKGRPTTLKTRIVAHGQQMVRFDKESRKPIPQASINKILEYVKSLREKIGAIV
                     VSDYSKGVISKELIEGIKKIVKNSKIFICLDPKQSNFSVYEGVHVITPNHHEAQRAAD
                     MEITNANDLQRLSETLLKKYDFQAMLITRGEEGMSLFEKGRKIVHTYFPAQAKEVYDV
                     TGAGDTVIGVLALGLAAKASMKEATCLANQAAGIVVGKVGTATVSQEELIEVL"
BASE COUNT          489 a          421 c          317 g          531 t
ORIGIN      
        1 tccacggcag agaacaaaac tttttgcctt ggtaaataag tgtcagatca gcataaattc
       61 catcccgcaa ataaggccgg tgagcgtaac ccttgccagt ggctaacact aaatggggct
      121 tggataaata tcccggatca atattaactt gccgcttctc atccaacgcc aatttatttt
      181 caatctcatt tgtcgaaaga tttatatccg gcaatgtctc cggcctgatt aatttttcca
      241 ttgccaaaaa gcgacggata agattttcgc ccatttccgg gaggtagtaa ttcgtataat
      301 caaagggcat taacgcactt acgaaatcgg gctgaccata tagtgcggac agcatgttta
      361 ttgtttcact caattgcgct cctgccgtgg caaaaacgct aaataccaac tttactggct
      421 cagccggagt cggtttgctc ataatacttc tattaattct tcctgcgaaa cggtggccgt
      481 accgactttg ccgacgacaa tacccgcagc ctgattagcc aaacatgttg cctctttcat
      541 actggctttt gcggccagac caagggcaag cacgccgatg actgtatcgc cggcaccagt
      601 tacatcataa acttctttgg cctgcgctgg aaaataggta tgaacaattt tccggccttt
      661 ttcaaaaagg ctcatcccct cttctcctcg cgtgataagc attgcctgaa agtcatactt
      721 ttttaaaaga gtttcactta atcgctgaag atcattagca ttggttattt ccatgtcggc
      781 ggcacgctgt gcttcatgat ggttgggagt aattacatgc accccctcgt atacggaaaa
      841 attgctttgc ttcggatcaa ggcagataaa tatttttgaa ttttttacta ttttttttat
      901 cccttcgata agctccttag aaataactcc tttgctgtaa tcagaaacaa caatagcacc
      961 tatcttctcg cgcagtgatt ttacatattc cagaatttta ttgatgctgg cttgaggaat
     1021 cggttttctg ctttctttat caaagcgcac catttgctga ccgtgcgcaa caattctcgt
     1081 tttcagagta gtcggtcttc ccttttcctc aacgataccg tttgtttcaa ttttccgctc
     1141 ccgcagttcc ataagcaatc ttttcccaat attatccaca ccaatgacac ctgcaacata
     1201 aactttaccg cccatcgaat agatgctgtt cagcacattg gcgcaaccgc ccagcaaaat
     1261 gctgtctttt tggacatcaa ccacgggcac gggcgcttca ggtgaaatac gtgaaacctt
     1321 tccccaaatg aaatggtcaa ccattacatc tccgacaaca aggacacccg tctgggaaaa
     1381 attggcaata atttctaatg cccttttttt cgttagaatc ttctgcacgt ttttaaaaca
     1441 actcctggga aatttaaccc taaaaagaaa aaagcgcttg ccgcgcttac attaacaaaa
     1501 agcgtcgtaa tgttattacc aagaccggta tttgtcaaat attttctatc atataaaggg
     1561 ataccgtcta aatcctccaa attatcggtc ggaaatcaag tttaacgatt atcttgacac
     1621 ctaagcgtca tcttcattat acttgcagtc gcaccccaat aaccaatttc ttatcaaggt
     1681 caatatcttg aaatagattt ctttaaaata tcgtaaagga gtcttctatg ttaaatgttg
     1741 agcaatttcg atgtggcg
//
LOCUS       DASVIM010000162         3120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_151326, whole genome shotgun sequence.
ACCESSION   DASVIM010000162 DASVIM010000000
VERSION     DASVIM010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151326"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..979)
                     /locus_tag="VF305_03305"
     CDS             complement(38..979)
                     /locus_tag="VF305_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7416194.1"
                     /translation="MKKFSKKTIFLTIAVVVIIIAAVLYFAAPAYLYNKAIAGLRKDA
                     GLTIKSVNIPDFKIVYAEGGTGDTIIMLHGFGGNKDNWPRFAKYFTPNYRVIIPDLPG
                     FGDSSKPQDAKYNIMSQVERLNLFVKELKLTKFNIVGNSMGGSIAGNYAVAYPEMVKT
                     LALFDSAGVESPQKSGRALLLEKGINPLVAKDVKGYDRLLEIVFNKPPQLPSIIKRYL
                     AKQAIKDAPLNEKIYNEITNTDINALESKLNKITAPTLIVWGDSDKVIDVSSVPIFEK
                     NIKGSKSAIIKECGHLPMMEKPQETASIYQDFLKGKN"
     gene            complement(1153..1521)
                     /locus_tag="VF305_03310"
     CDS             complement(1153..1521)
                     /locus_tag="VF305_03310"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7416195.1"
                     /translation="MKINKINFEEKFAKLPNEDYAVRIIAKMNNYEFKIVKRKDDFIW
                     HKHEDTDETFIILDGTLVMNFRDRIVEVNKGEMIVIPKGIEHKPSSSNGYKALLIEPE
                     GVPNTGNVKSDLTIKKIEWI"
     gene            1900..>3120
                     /locus_tag="VF305_03315"
     CDS             1900..>3120
                     /locus_tag="VF305_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7416196.1"
                     /translation="MRAKKKVYAPGECYDYQLLIKHILETPLIYAPHREIVYRDKVRL
                     TYKDLNERIHRLASGLERLGVKNGDTVCVFDYDSHRYLESFFAIPMMGAILHTQNWRF
                     SPEQILYTMNHAQDKVVLIHMDFLPILEAVWDKLTSVKKVVLIADNSQKPHSKIPFDG
                     EYEEMLAQAAPFYDFPDFDEKTRATTFYTTGTTGLPKGVYFSHRQLVLHTQAGLISQG
                     AFESISRFRSNDVYMPLTPMFHVHAWGVPYGATLLGVKQIYPGRYEPEMILKLIEKEK
                     VTYSHCVPTILQMILSSPVAKKTDLSSWKVIIGGASLTKGLALSAMEKGIEIYASYGM
                     SETCPLLSRATLKYWMLNKPKEELVDTLIKTGLPAPFVYMRLFDPMGREVPHDGVSMG
                     EVCVRTPWLTEGYHN"
BASE COUNT          907 a          671 c          587 g          955 t
ORIGIN      
        1 cttttaatat acctcaaccc gatgggcggg tactgcgcta atttttcccc tttaaaaaat
       61 cctgatatat tgaagctgtt tcctgcggtt tttccatcat gggaagatga ccgcattctt
      121 ttataatagc tgatttagaa ccttttatat ttttctcaaa aatcggaaca gaggatacgt
      181 caataacttt gtccgaatct ccccatacta ttagtgtggg ggccgtaatt ttatttaatt
      241 tgctctccag tgcgttaata tctgtattcg taatttcatt gtatattttt tcgttaagag
      301 gcgcgtcttt tattgcttgt ttcgctaaat atctttttat tatggaaggc aattgaggtg
      361 gtttgttaaa aacgatttct aacaatctat catatccttt tacatcttta gcaacaagag
      421 gattaatgcc tttttccaaa agcaaagcgc gtccgctttt ttgcggagat tcaacgcctg
      481 cggaatcgaa tagtgcgaga gttttaacca tctccgggta agccactgca taattaccgg
      541 cgatactgcc gcccatagaa tttccgacta tattaaattt tgtcagtttc aattctttta
      601 cgaacaaatt caatctttcc acctgagaca tgatgttgta tttcgcgtcc tgcggtttgg
      661 aactgtcacc gaaaccggga agatcgggaa ttattacccg ataattgggc gtaaagtatt
      721 tcgcgaacct cggccagttg tctttgtttc cgccaaagcc gtgaagcata attattgtgt
      781 cgccggttcc gccctctgcg taaacaattt taaaatcagg aatgttcacc gatttaatcg
      841 tcagcccggc gtctttgcgc aagccggcaa ttgctttatt gtataaatat gccggcgcgg
      901 caaaataaag caccgccgca ataattatta caacaactgc aatcgtcaaa aaaattgttt
      961 tcttcgaaaa ctttttcatt tagaaacctc ccagtaatta tttattcaaa atatttgaca
     1021 agaactttct ttgggtagaa agaaatatat tgaaaaaacc cttcaatgtc aaagagaaat
     1081 aatgaccaca ccaattgccc cgaatagata aaatgttacc agggggtaga taaaaagcgc
     1141 tctattccct aattaaatcc attcaatttt ctttatggtc aaatcacttt tcacatttcc
     1201 ggtattgggc acgccttccg gctcgattaa aagagcttta tatccattgc ttgatgacgg
     1261 cttatgttca atacctttag ggatcacaat catttcacct ttattgactt ctacaatacg
     1321 atcccgaaag ttcataacca atgtaccatc caaaattatg aaagtctcat cggtatcttc
     1381 atgtttatgc caaataaagt cgtctttacg ttttactatc ttgaattcgt agttgttcat
     1441 cttcgcgata attctcaccg cgtaatcttc gttcggaagc ttagcgaact tttcctcaaa
     1501 gttaatcttg ttaattttca tattctcttt cctgttaaca ttgttcatat gtctctttat
     1561 aaaaacctaa acactgcaaa ttatgcggta taacaccata gaaaatacat gaaacaatta
     1621 accgtaactt gataatatat atttattatc aataaagtta ataactgttc aactggatat
     1681 tatatagatc tgtataatat cctcaaatat gataaattgt gttgaaatat tatctatatt
     1741 tttcttgacc tttgtcaata atatttatca aaatcattgt ccacagtaaa attattttga
     1801 catccaaaaa ctgttcacct atgctcagtt cattgtcaaa aattaataac aaactgcaac
     1861 atcaataagt ggaattttta ttttagggag aagaagtcca tgagggcgaa gaagaaagta
     1921 tacgcaccgg gcgaatgcta cgattatcag ttacttatca agcatatcct ggaaacaccg
     1981 ctgatttatg ctccccatcg ggaaattgtt tatcgggata aggtaagact tacatataaa
     2041 gacttgaatg aacgtattca ccggctggct tccggcctgg aaaggctggg cgtaaaaaac
     2101 ggagatactg tgtgcgtctt cgattatgac agccaccggt atctggaatc tttttttgcc
     2161 atccccatga tgggagcaat cctgcacact cagaactggc gtttttcccc ggaacaaatt
     2221 ctttacacca tgaaccatgc acaagacaaa gtggttttga ttcacatgga cttcctaccc
     2281 atactggaag ctgtctggga taaactgacg agtgtaaaaa aggtggtttt aattgcagac
     2341 aacagccaaa aaccacactc caagatacct tttgacggcg aatacgaaga aatgctggct
     2401 caagcggcgc cattttatga ttttcccgat ttcgatgaaa aaacgagggc aacgacattc
     2461 tacacgacag gaaccacggg gttgcccaaa ggagtatatt tttcccaccg gcaacttgtc
     2521 ctgcatacac aggccggcct gataagccag ggagcattcg aatccatcag ccgcttcaga
     2581 tcgaatgatg tttatatgcc tcttacgccg atgttccatg tccacgcctg gggtgtgcct
     2641 tatggggcaa ccctgctcgg cgtcaaacaa atctatcccg gtcggtatga accggaaatg
     2701 atcttgaaac tgattgagaa ggaaaaagtg acttattcgc attgcgtccc cactattctc
     2761 cagatgattt tgagctctcc cgtcgcgaag aaaaccgatt tatcctcatg gaaggtcatt
     2821 attggtggtg ccagtctgac caaaggactg gccctttctg ccatggaaaa gggaatcgaa
     2881 atatatgctt cctatggtat gtccgaaacc tgtccccttc tttccagggc caccctcaaa
     2941 tactggatgt tgaataaacc aaaagaagaa cttgtggata ctctcatcaa aaccggtttg
     3001 ccggcacctt tcgtatatat gcgtctcttt gatccaatgg gaagagaggt gccccatgat
     3061 ggcgtctcga tgggtgaggt ctgcgtccgg acaccatggc tgactgaggg ctatcataac
//
LOCUS       DASVIM010000163         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_747736, whole genome shotgun sequence.
ACCESSION   DASVIM010000163 DASVIM010000000
VERSION     DASVIM010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747736"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1222)
                     /locus_tag="VF305_03320"
     CDS             complement(80..1222)
                     /locus_tag="VF305_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7416197.1"
                     /translation="MLKNIFRVIFALLFTVLIAGTVFAAPPIKIGALFSVSGPAAFLG
                     EPERNTAQMLVNEINKAGGINGRQLELIVYDTQGDATKAVQAVNKLIKDDKVVAIIGP
                     STTGDSMAVIPIIEKAEIPLISCAAGIKITDPVKKWVFKTAQNDALAVARIFEYLKKQ
                     KINKIAVLTVSDGFGSSGREQLVLQAKESGFQIISDETYGPKDMDMTAQLTKIRGSNA
                     QAIICWGTNPGPAIVARNVKQLGIKLPLFMSHGISSKKFIELAGDAAEGIILPSGRVI
                     VANQMPNKDPQKKALLSYVNSYQKLYKVEGDHFGGHAWDAMMLLKGAMEKGGDSSAAI
                     RDQLEKTTKFAGIGGTFSYSPQDHAGLNKDAFVLVQIREGDWKLIK"
     gene            complement(1573..>1806)
                     /locus_tag="VF305_03325"
     CDS             complement(1573..>1806)
                     /locus_tag="VF305_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7416198.1"
                     /translation="WNQDRSIWMVISDTGCGIPQESLSKIFEPFFTTKDVGKGTGLGL
                     SITYEIIQRHKGDITVKSKVGRGTTFTVRIPVV"
BASE COUNT          480 a          414 c          340 g          572 t
ORIGIN      
        1 tttcgataaa tattaatgcg gcgcaggcat catcatcgcc cgcaccgctt ataaagttta
       61 actaatttat atcacttcat catttaatca atttccagtc gccttctctg atttgcacca
      121 gaacaaaagc gtctttattt aatccggcat ggtcttgcgg agaataactg aaggttccgc
      181 cgataccggc aaattttgtt gtcttttcca gttgatcacg aatagccgct gaagagtcgc
      241 cgcctttttc catagcgcct ttgagcagca tcatcgcgtc ccaggcatgc ccgccaaaat
      301 gatctccttc aactttgtaa agcttttgat aactatttac atagctaagc agcgcctttt
      361 tttgcgggtc tttattgggc atttgattgg caacaatgac acgaccggaa ggaagaataa
      421 ttccctcagc ggcatcaccg gcaagttcaa taaatttttt ggaggaaata ccatggctca
      481 taaacaaagg gagttttatg cccagttgtt ttacatttct ggcgacaatg gctggtccgg
      541 ggttggtgcc ccagcaaata atggcctgcg cattactacc gcgaattttt gtcaattgag
      601 cggtcatgtc catatctttg gggccgtaag tttcatcgga aattatttga aacccggatt
      661 ctttggcttg taaaaccaat tgctctcttc ctgatgaacc aaagccatcc gacacagtca
      721 gaacagctat tttattaatt ttttgtttct ttagatactc aaatatcctg gctacagcca
      781 gcgcatcatt ttgtgcggtt ttaaaaaccc atttctttac agggtcggta atcttaattc
      841 ccgcggcgca ggaaataagc ggtatttcgg ctttttcgat tatgggaatt acagccatgc
      901 tatcaccggt cgtgctgggg ccgataatag caaccacctt atcgtcttta atcaatttgt
      961 tgactgcctg cacagctttt gtggcatcac cctgagtatc ataaactata agctccagtt
     1021 gacgcccgtt tattccaccg gctttgttta tttcgttcac cagcatctgc gctgtattgc
     1081 gttccggttc acccaaaaag gctgccggtc cggagacaga gaatagcgct ccgatcttga
     1141 tgggcggagc ggcaaagact gttccggcta ttaaaacagt aaacagtaaa gcaaaaatta
     1201 ctctgaaaat attttttaac ataaacacct ccctttaaaa tatttgcccc cttataaatt
     1261 tatgaaatta aaaaagccac ggatattttt taatctgccc gtggcttgtg tacgatctat
     1321 tttcgagtcg tctcagcgat gagcagatcg agacaggaca aggtaataat agtaataacc
     1381 ataatggtta gtgtccaatg tgtcgtttac cttgtgttcg ttcctgctaa ccatttgctg
     1441 tagactcctt aatatttttg ctaatttgta aatcatagat aaaacttcat gtcaaacgaa
     1501 aaatgacata ttatgatttc tccgttggaa aacatccgga ttaattccca gtagttcatt
     1561 catgttattt tgctatacaa ccggtattct taccgtaaat gtcgtccctc ttcctacttt
     1621 acttttaacc gtaatatccc ctttatgtct ctggataatt tcataggtaa tgctaagccc
     1681 tagacctgtt cccttaccca catccttagt cgtgaagaag ggttcaaata ttttactaag
     1741 actctcctgc ggaattccac aacctgtatc ggatatcacc atccagattg atctgtcttg
     1801 attcca
//
LOCUS       DASVIM010000164         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_834482, whole genome shotgun sequence.
ACCESSION   DASVIM010000164 DASVIM010000000
VERSION     DASVIM010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834482"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF305_03330"
     CDS             complement(<1..215)
                     /locus_tag="VF305_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416199.1"
                     /translation="MKLRSKLFIVTSALLTSFTLLALSADCRNVLAQTPSDAVKVTFV
                     EIGSVNCIPCKAMQPIMKAVEEEYKGQV"
     gene            complement(243..1292)
                     /locus_tag="VF305_03335"
     CDS             complement(243..1292)
                     /locus_tag="VF305_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX7416200.1"
                     /translation="MTECCTTDNGNKKKINYYIPFLLIPFTVLIYIFLEQGVDYLVFR
                     ILQLDKSAHLTEAIRFFLYDTPKVFILLTVIVFVVGIIRSFFSPEKTRKALEGKPLFI
                     GNIMASTLGIVTPFCTCSAIPLFIGFVESGIPLGVTFSFLVAAPMINEVALVLLFGLF
                     GWKTALLYASTGLLIAIVSGWVIGKLKVERFVEEWVYEIKAGDFRMEERKVTFNDRIQ
                     AGHTAVVDIVSKVWIYIVAGIAVGAAVHGYVPENFMASIMGKEAWWSVPLAVLIGVPL
                     YSNAAGIIPILQALLEKGASLGTALAFMMSVIGLSLPEMIILKKVIKLPLIIIFVSVV
                     ATGIMIVGFLFNLIY"
     gene            complement(1332..1568)
                     /locus_tag="VF305_03340"
     CDS             complement(1332..1568)
                     /locus_tag="VF305_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7416201.1"
                     /translation="MKIEILGTGCATCNKLEDTVRLAIKEMGIDAQIDHVKDIKKIMT
                     YGVMTAPALVIDGKVKVAGKLPSLAEMKQLIGDK"
     gene            complement(1587..1919)
                     /locus_tag="VF305_03345"
     CDS             complement(1587..1919)
                     /locus_tag="VF305_03345"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7416202.1"
                     /translation="MKSLVKTQHEARAKIIKAMAHPSRLFIIEELNKKERCVNELTSM
                     IGADASTVSKHLSVLKNAGLVADEKRGNSIFYSLRCPCIMQFMSCLEDVLSSNAKQHI
                     EILKYCKR"
     gene            complement(2018..>2706)
                     /locus_tag="VF305_03350"
     CDS             complement(2018..>2706)
                     /locus_tag="VF305_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HEX7416203.1"
                     /translation="IKDILPRENDYLIGYANLADLLPAKYKSCVYAIVLARRLNDRII
                     DAIIDGPNIEYYTHYNEVNLELAKVAHGLADTIRLQGSKSLVIEPTIQDKDLDKNFFK
                     TLRFDFSHKMAATRAGLGWIGKTALFISGKYGPRVRLVTVLTDYPLSYCTEPINESQC
                     GSCDLCVRHCPAQAANGKPWNIHVDRNEFFDPFACRKKALELSYQNFNKEISICGKCI
                     VVCPIGKIQR"
BASE COUNT          788 a          587 c          564 g          767 t
ORIGIN      
        1 acctgtccct tgtattcctc ttccacagct ttcatgatcg gctgcatagc cttgcaggga
       61 atgcagttga ctgaaccgat ttcaacgaag gtcactttaa ctgcatctga tggtgtttga
      121 gcaaggacat tccggcaatc agccgaaagc gcgagtaatg taaatgatgt aagcagagcc
      181 gaagttacaa taaataattt tgaacgcagt ttcatttcca ataatctcct taatattgaa
      241 ttttaataga ttagattaaa cagaaatcct acaatcataa tgcctgtggc aacgacactg
      301 acaaaaatta tgatcagggg gagcttaatt actttcttca agatgatcat ttccggaagt
      361 gaaagaccga tcaccgacat cataaaggcc agcgcggtgc ccagggatgc gcccttttcg
      421 agcagtgcct ggagaatcgg gatgatgccc gccgcgttgg aatacagggg aacgccgata
      481 agcaccgcca gaggaacaga ccaccacgct tccttgccca taattgaggc cataaaattt
      541 tccggcacat agccgtgaac ggcagcgccc acagcaatgc ctgccacaat gtaaatccag
      601 accttggaaa ctatatcgac aacggcagta tggcccgcct gaattctatc gttaaaagtt
      661 acttttcttt cttccattcg aaaatcaccg gctttaattt cataaaccca ttcttcgacg
      721 aagcgctcta ctttaagttt gccaatcacc cagccggaaa cgatagcaat aagcaatccc
      781 gtggaagcat aaagcagagc agtcttccag ccaaaaaggc caaagagcaa gaccagcgcg
      841 acttcattaa tcatcggagc ggcgacaaga aaagagaagg tgacgcccag tggaataccg
      901 ctttcgacaa agccgatgaa aaggggaatt gccgaacagg tacaaaacgg tgttacgata
      961 ccaagagtgc tggccatgat attaccgata aatagcggct ttccctccag agcttttctg
     1021 gttttttccg gcgaaaaaaa agagcggata attcccacaa caaaaacaat aacggtaaga
     1081 aggataaaca ctttgggggt gtcatagaga aaaaatctga ttgcttccgt cagatgtgct
     1141 gatttatcca actgtaatat tctaaagaca agataatcaa ctccctgctc taaaaatata
     1201 tagatcagaa ctgtaaaagg aatcagcaaa aaaggaatat aataatttat ttttttcttg
     1261 ttgccattat ctgtagtgca gcactcagtc attttgacta ttccttattt attcagtttt
     1321 atgaatagag tctatttatc gccaatcagt tgtttcattt ccgcaagaga tgggagttta
     1381 ccggcaactt taacttttcc gtcaatcacg agagcgggag ctgtcattac gccgtaggtc
     1441 ataattttct ttatgtcctt gacatgatct atctgcgcgt caatacccat ctccttaatg
     1501 gcaagacgaa ctgtatcttc aagtttgtta caggttgcac aaccagtacc taatatttct
     1561 atcttcatag ctttagctcc ttaaacttat cttttacagt attttaaaat ttcgatgtgc
     1621 tgtttggcgt tggaagaaag cacatcctcc aaacagctca taaattgcat aatacagggg
     1681 cagcgcaggc tatagaaaat agagttgcct cttttttcat cggcaacaag ccccgcattt
     1741 ttcaaaacgc tcaagtgttt ggaaaccgtt gaagcatccg ctccgatcat agaggtgagt
     1801 tcattaacac aacgttcttt tttgttgagc tcctcaataa taaatagtct gctgggatgc
     1861 gccatagctt taatgatttt tgcccgcgct tcatgctggg tttttactaa acttttcatt
     1921 ttaacttccc caagtatcta tttggtaata tagccaaata gccatggatg tcaacaatta
     1981 tttttattga taatatgtta tcactgctta acctttatta tctttggatt ttccctatcg
     2041 ggcatacgac aatgcatttg ccgcatatac tgatttcttt attaaaattt tgataagaaa
     2101 gttcaagcgc ttttttccgg caggcaaatg gatcgaagaa ttcattacgg tcaacgtgga
     2161 tattccaggg cttgccgttg gccgcctgcg ccgggcagtg ccggacacaa agatcacagg
     2221 agccgcattg gctttcgtta attggttcgg tgcagtagga taaggggtaa tcggtaagca
     2281 ctgtaaccag acgcacgcga gggccgtatt ttccggagat aaaaagagct gttttgccga
     2341 tccagcccaa accggcgcgt gttgccgcca ttttatgaga aaagtcgaag cgcaaggtct
     2401 tgaaaaaatt tttatcgagg tctttgtcct ggattgttgg ttcaatgacc aatgatttac
     2461 tgccctgcag acgtattgta tctgccaagc catgcgcaac ttttgcaagc tcaaggttta
     2521 cttcgttata atgagtatag tattcgatgt tcggaccatc aatgatagca tcaattatcc
     2581 ggtcgttgag cctgcgggct aaaacaattg catagacgca agatttgtat ttcgcaggca
     2641 ataggtcggc tagattggca taaccgatga ggtaatcatt ttctctgggt agaatatctt
     2701 taatta
//
LOCUS       DASVIM010000165         4146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1261968, whole genome shotgun sequence.
ACCESSION   DASVIM010000165 DASVIM010000000
VERSION     DASVIM010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261968"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VF305_03355"
     CDS             <1..425
                     /locus_tag="VF305_03355"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX7416204.1"
                     /translation="ACGAHTIMVGGLFAGTEESPGETVLFQGRSYKVYRGMGSLEAMK
                     LGSRDRYYQDDIDDIEGPSKLVPEGIEGRVPFRGSLSASILQLIGGLKAGMGYVGCKT
                     IPELMEKARFVRITQAGLRESHVHDVIITKEAPNYWLD"
     gene            648..4112
                     /locus_tag="VF305_03360"
     CDS             648..4112
                     /locus_tag="VF305_03360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX7416205.1"
                     /translation="MKHSDFVHLHVHTQYSLLDGMIRLEDLFQKAREYKMPAIAITDH
                     GNMFGAIDFYQQAYKQGIKPIIGCELYVAPRSRLDKSYSIGETARHLIVLVKNMQGYK
                     NLMKLTTAGYLEGFYYRPRVDKEILKEYSEGLIASSACLHGEINDLILKGKMDEAKKA
                     ASSYQAIFGEENFFLELMENGIPEQKIANKGLIEISKELSIPLVATNDCHYLNREHAE
                     AHDVLLCIQTGKTIEDNDRMRLVTDQFYFRSPEEMHELFSHTPEALSNTVSVAERCNL
                     SLEFGKFYLPNFEIKNPEETLNDYLERKAREGLQKLMPDILKDQKESEPEIREKYEKR
                     LRDELEIIKAMGFAGYFLIVSDFVKHAKHNNIPVGPGRGSAAGSLVAYALKITTIDPI
                     RYALFFERFLNPNRITMPDIDIDFCQEGRDEIIKYVTEKYGKDKVSQIITFGKMQAKA
                     VIRDVGRALNIPYSEVDKIAKLVPNVLNITLDGAIKTEPRLAQEAKNNPQVAKLLSLS
                     RILEGLNRHASTHAAGVVISDVPLVERVPLCIPKDEIVSQYPMNDIQTVGLTKFDFLG
                     LKTLTVIKNAVNFIKESNNIELDINNLPLNDKQTYELLMRGETDGVFQLESDGMKDIM
                     KNLKPDRIQDVIALIALYRPGPMKMVPEFISRKQGKTKISYELPELEAILKETYGIIV
                     YQEQVMQIANVIGNYTMAEADTLRKLMGKKQFSVIKEKEKPKFLEGAKKQKINENKAK
                     VIWDQMETFGEYGFNKSHSTAYAMITYQTAYLKANYPVAFMAALLTSEKDNRDKIIRY
                     MSSCKEMGINILPPDINESFKDFSISGENIRFGLAAVKNIGHAAIDSIISAREKGKFS
                     SFMDFLTRTDLRKTNKRVIESLIKCGAFDSLGYKRRQLMQYYEEAMDTAQRRQKETQS
                     SQSSFFDELDSGSSIKNGIKSNEIPDVSEWDQKELLSVEKEALGFYISGHPLLRFADR
                     LKLVTNSDSSNLNTKKDKDTVTIAGVISSLAEKQTRRKDIMCNVTLEDLQGSVNIIFW
                     ADTYKKYYSVLHEDEPIVIQGIIDIGDESLKIIAQEAVALSKALENPYKQVRFMVDAD
                     KVSSEIIISLNETIKKYHGNHDGYLHIINGKSETIIYLGDQMRLDINEKLKKEADSIL
                     GEGATIYS"
BASE COUNT         1323 a          810 c          923 g         1090 t
ORIGIN      
        1 ttgcctgcgg cgcacacact ataatggtcg gcggattgtt tgccggcacg gaagaaagcc
       61 ccggagaaac tgttttgttt caaggacgaa gctacaaagt ttaccgtggc atgggttcac
      121 tcgaagcgat gaaactgggt agccgcgatc gttattatca agatgatata gatgatatag
      181 aaggcccatc gaagctagta ccggagggaa ttgagggcag ggtgcctttc cgcggttcac
      241 tttcggcgag tattctccag cttatcggcg gattaaaggc ggggatgggt tatgtcggtt
      301 gtaaaacaat cccggaattg atggaaaagg ctcgctttgt ccgaattacc caggccggct
      361 tgagagaaag ccacgttcat gatgtaatca ttaccaaaga agcgcctaat tactggctgg
      421 attagaagtt cagttcttta ttaatatatt cctctttatt gccactgatg aaatatacca
      481 tttctaaatc aaagatgata tcgaggcggc aaggaggagg cgacgacgca tataacctga
      541 aggtcacgcg ttgtacatac gttgaggaag ccgacgacgc cccaggcatg cgccgggctt
      601 tgttgccaac aaagatagcg ctttgattta gaattggaat tataattatg aaacactcag
      661 atttcgtaca ccttcatgtt catacccaat acagtcttct ggacggaatg attcgcctcg
      721 aagatctttt ccagaaagcg cgcgaatata agatgccggc tatagctatt accgaccatg
      781 gcaatatgtt cggggcgatt gatttttatc agcaggccta caagcagggg ataaagccga
      841 taatcggctg tgaattatat gtagcgcctc gtagccgcct ggacaaaagc tactctatcg
      901 gtgaaacggc aaggcatttg attgttctgg taaaaaacat gcagggctat aaaaatctta
      961 tgaagctgac aaccgccggc tatctggaag gtttttacta caggccgaga gtggataaag
     1021 aaatactaaa agaatattcc gaaggcctta tcgcttccag cgcttgtctg catggtgaaa
     1081 tcaacgattt aatccttaaa ggcaagatgg atgaagctaa aaaagcggcg agttcctatc
     1141 aggcaatttt tggtgaagag aatttctttt tggaacttat ggaaaatggt atcccggaac
     1201 aaaaaattgc caataaaggt ttgattgaaa tcagcaaaga attatccatt ccccttgtag
     1261 ccactaatga ttgtcattac ttaaaccgtg aacatgccga ggctcatgat gttttgctct
     1321 gtatccaaac cggcaagact atcgaagata acgaccgcat gcggttagtc acagatcaat
     1381 tttattttcg ttcacccgaa gaaatgcacg agcttttttc ccatactccc gaagctttat
     1441 ccaatacggt gagtgttgcc gaaagatgta atctttcctt ggaattcggc aagttttatc
     1501 tgccgaattt tgagattaaa aatccggaag aaactttgaa tgattacttg gaacgcaagg
     1561 cgagggaagg gcttcaaaag ctcatgccgg atattttaaa agatcaaaaa gaaagcgagc
     1621 ctgaaatccg ggaaaaatat gaaaaacgtt tgcgcgatga gttggaaata atcaaggcaa
     1681 tgggttttgc gggatatttt ctaatcgttt ccgattttgt taaacacgcc aagcacaata
     1741 atattcccgt agggccggga cgcggctcgg cggcaggctc tcttgtggcc tatgctttaa
     1801 aaatcaccac tatcgatccc atccgttatg ctttgttttt tgaacgattt ttaaatccca
     1861 accgtatcac catgcccgat atcgatatcg atttttgcca ggagggcagg gatgaaatta
     1921 ttaagtacgt aaccgaaaaa tacggcaaag ataaagtttc ccaaattatc acctttggaa
     1981 aaatgcaggc caaggcggtt atacgcgatg tcgggagagc cctgaatatt ccctatagcg
     2041 aagtggataa aattgccaaa ttagttccta atgttttgaa tattaccctt gatggagcga
     2101 taaaaacgga accaaggctt gcccaagagg ccaaaaataa tcctcaagtg gccaaacttt
     2161 tatctttatc gagaattttg gaaggtttga accgccacgc gtccactcac gctgccggag
     2221 ttgttatatc cgatgtgcct ctggtggaaa gagttcctct ttgcatacct aaggatgaaa
     2281 ttgtttccca atatccaatg aatgacattc aaaccgttgg cttgaccaaa tttgatttcc
     2341 tgggattaaa aacattgacc gtcattaaaa atgcggttaa ttttataaaa gaatcaaaca
     2401 atatcgaatt agatattaat aacttacctc ttaatgataa acaaacatat gaactcttaa
     2461 tgcgaggcga aacagacggc gtgttccaat tggaaagcga cggcatgaaa gatattatga
     2521 aaaatttaaa gccggaccgt atccaagatg tcattgcttt gattgctctg taccgtccgg
     2581 gccccatgaa aatggttccg gaatttatca gccgtaagca gggaaaaaca aaaattagct
     2641 atgagcttcc tgaattggaa gcgattctca aggaaacata cggaataatt gtttatcagg
     2701 aacaggttat gcagattgcc aacgtaatcg gcaattacac gatggccgaa gccgacacct
     2761 tgcgcaagct gatgggtaaa aaacaatttt ccgtgataaa ggaaaaggag aaacccaagt
     2821 ttctcgaagg cgcgaaaaag caaaaaatca atgaaaacaa agcaaaagtc atctgggatc
     2881 agatggagac gtttggcgaa tacggtttta acaaatccca tagcaccgcc tacgccatga
     2941 tcacgtatca gacggcttat ctcaaggcaa attacccggt cgctttcatg gctgctttgc
     3001 tcaccagtga aaaagataac cgcgataaaa taataaggta catgagttct tgtaaagaaa
     3061 tgggcataaa tattctgccg ccggatataa atgaatcatt taaagatttt agcatatcag
     3121 gagaaaatat ccgttttggt ctggctgccg ttaaaaatat cggccatgcc gccattgatt
     3181 cgattatttc cgcccgggaa aaaggcaaat tttcatcatt tatggatttt ttaacacgca
     3241 cggatttgcg caagacaaat aaaagagtta tcgaaagttt aattaaatgc ggcgctttcg
     3301 attctttagg ctacaaaaga cgccaactca tgcaatatta tgaagaggca atggatacgg
     3361 cacagcgcag gcaaaaagaa actcaaagct cacagtcgag tttttttgat gagttagatt
     3421 ccggttcttc cataaaaaat ggaataaaat caaatgaaat acctgatgtt tccgaatggg
     3481 atcagaagga actcttatct gtcgaaaagg aggcattggg cttttatatc tccggccatc
     3541 ccttactgcg ttttgcggat cgtctaaaat tggttacaaa cagtgattcc agtaacttaa
     3601 acaccaaaaa ggacaaggat accgttacaa ttgccggagt aatcagcagt ttggccgaaa
     3661 agcaaaccag gagaaaagat ataatgtgta acgttaccct ggaggattta caaggttccg
     3721 taaatattat attttgggca gacacctata agaaatatta ctccgtattg catgaagatg
     3781 agccgattgt tattcaggga attattgata ttggggatga gtcactaaag atcattgccc
     3841 aggaggcagt agcgctttct aaagcactgg aaaatcctta caaacaagta cgcttcatgg
     3901 ttgacgcgga taaagtttct tctgaaatta tcatatccct gaacgaaaca ataaaaaaat
     3961 atcatggtaa tcacgacggt tacctgcata ttatcaacgg gaaaagcgaa acaataatat
     4021 atttagggga tcaaatgcgc ctcgatatta atgagaagtt gaaaaaagaa gcggatagta
     4081 tattagggga gggcgctaca atttactctt aataccattg tcattgcgag cctagtgaag
     4141 caatct
//
LOCUS       DASVIM010000166         1710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1176855, whole genome shotgun sequence.
ACCESSION   DASVIM010000166 DASVIM010000000
VERSION     DASVIM010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176855"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="VF305_03365"
     CDS             complement(<1..1500)
                     /locus_tag="VF305_03365"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7416206.1"
                     /translation="MVNKTKKLLEKIIYCLTITVLALIYGNCYASVEILNVRHWTAPD
                     HTRIVLDVDNETVWDVKESENLLVLNFKDASLNKSIPAEIILNKPGIKKLVFYHGDKD
                     GVKIELILDKYRKAEVFKLKKFQDRPNRIVVDIILEQGSKEGKITDMFSELKQKKIIV
                     IDPGHGGEDPGAVGKRRVYEKNIVLLISREIKKTINKMPGYHAVLTRDGDYYVSFSKR
                     LQTAKDLGASLFVSIHADAARNHKAKGSSVYCLSTGAASNEAAKLLAKNENLSDIFGG
                     VPGGESSNESSQIIMNMFQTNTINLSKIYAGILMKHLDMVNYLKYQSVQEAPFRVLKL
                     PDIPAVLIETAYISNPQEESLLKKSSFRKTLAIAISSSIREYLSGTAAIPQSIDAFEE
                     DNKTGKANKKQNDDEITTIYYKIKRGDTLFSVAGYFNTKVAVLLKLNNLKLKDQLFVG
                     RKILVPANKTGDNEFAVSGQEKKYSNRKKPSRIYKVKKGETLFSLAKHNS"
BASE COUNT          476 a          359 c          297 g          578 t
ORIGIN      
        1 ggaattatgt ttggccaaag aaaaaagcgt ttctcctttt ttcaccttat aaatccttga
       61 aggctttttc cgattactat atttcttctc ttgacctgat acggcaaatt cattatctcc
      121 tgttttattc gccggcacca atattttccg gccgacaaaa agctgatctt ttaattttaa
      181 gttattaagc ttcaaaagca ccgcaacttt tgtattaaaa taccccgcca ctgaaaaaag
      241 cgtatcacca cgttttattt tataatagat tgtagttatc tcatcatcat tttgcttttt
      301 gtttgcttta ccggttttat tatcttcttc aaatgcatca atactttgag gaatggcagc
      361 agtgcctgat aaatactctc ttattgagga ggatatagct atcgccagcg tctttcgaaa
      421 actacttttt tttaacaaac tttcctcttg aggattggat atgtatgctg tctcgattaa
      481 taccgccgga atatccggca atttcaacac gcggaaaggt gcttcctgaa cgctttgata
      541 ttttaaataa ttgaccatat caaggtgctt cattaaaatg ccggcataaa ttttcgacaa
      601 attaatcgta tttgtttgaa acatgttcat tataatctga gaggattcat tgctgctttc
      661 gccgcccggt acaccaccga agatgtcgga taagttttca tttttggcca gcagttttgc
      721 ggcttcatta ctagcagcac cggtggagag gcaatatact gagctacctt tggccttatg
      781 gtttctggcc gcatctgcat gaatgctgac aaataggctg gcgcccaaat ccttagctgt
      841 ttgcagccgc ttgctaaaag aaacataata gtcaccgtca cgcgttaaga ctgcgtggta
      901 tccaggcatt ttattaatgg tttttttgat ttcacggcta attagaagca caatattttt
      961 ttcataaact cttctttttc ctactgctcc cggatcttca ccaccatgcc cgggatcaat
     1021 aacaattatt tttttctgtt tgagttcgga gaacatgtcc gttatttttc cttctttaga
     1081 accttgttcc aaaataatat ccacaacaat ccggttaggc ctatcctgaa acttttttaa
     1141 cttaaagact tccgctttcc ggtatttatc caaaattaat tcaattttta caccgtcttt
     1201 atcaccatga taaaaaacaa gctttttaat ccccggttta tttaagatga tttcagccgg
     1261 tattgattta tttaaggagg catccttaaa atttaaaacg agcaggtttt cgctttcctt
     1321 aacatcccaa acggtttcgt tatccacgtc cagaacaatt ctcgtatgat caggcgccgt
     1381 ccagtgacga acattcaata tttctacgga cgcataacaa ttaccatata tcaaagccag
     1441 tactgtaata gtcaaacaat aaataatttt ctctaaaagc tttttcgttt tattcaccat
     1501 ggcttaaaat agcaaattta ttcagaaact gcaactgctc gatagaaaag atggggttaa
     1561 aaatggattt ggccgcgaag tcttggctct tcatctttac cgaaatcata tatttcattg
     1621 aacattgggc ttaggtagtg tagaaactca aggcgtgggc gattagctca gttggataga
     1681 gtgctggcct ccgaagccag atgccgcgag
//
LOCUS       DASVIM010000167         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_835633, whole genome shotgun sequence.
ACCESSION   DASVIM010000167 DASVIM010000000
VERSION     DASVIM010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835633"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /gene="rplP"
                     /locus_tag="VF305_03370"
     CDS             complement(<1..398)
                     /gene="rplP"
                     /locus_tag="VF305_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX7416207.1"
                     /translation="MLQPKRTKHRKMQKGRMKGDTKRGASIAFGTFGLKALEEVWLTN
                     RQIESARQALTRHMKREGNVWIRIFPDKAITKKPAEVRMGKGKGNPEYFAAVVQPGRI
                     IFEADGVSEQIAKEAFELAAQKLPIATKFVV"
     gene            complement(720..1346)
                     /gene="rpsC"
                     /locus_tag="VF305_03375"
                     /pseudo
     CDS             complement(720..1346)
                     /gene="rpsC"
                     /locus_tag="VF305_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(1575..1883)
                     /gene="rplV"
                     /locus_tag="VF305_03380"
                     /pseudo
     CDS             complement(1575..1883)
                     /gene="rplV"
                     /locus_tag="VF305_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
BASE COUNT          452 a          467 c          274 g          690 t
ORIGIN      
        1 acaacaaatt ttgtagcaat aggtaatttt tgagctgcta attcaaaagc ttcttttgca
       61 atctgctctg aaacaccatc tgcttcgaaa ataattctgc caggttgcac aacagcggca
      121 aaatattcag gattcccttt acccttaccc atccttactt cagcaggctt tttcgtaatt
      181 gccttatcag gaaatattct tatccaaaca tttccttcac gtttcatatg acgggttaat
      241 gcctgccttg ctgattctat ttgcctgtta gttaaccaaa cttcttcaag agcttttaag
      301 ccaaacgttc caaaggctat tgaagcgcca cgcttagtat cacctttcat acggcctttc
      361 tgcatctttc tatgcttcgt tctttttggc tgtaacataa attaatagtt gaattattaa
      421 attgttgaac ctgcctatcg gcagacaggt gttaaaaaat attttacctc aaaacatttt
      481 caaaaaaatt atcttcttcc tccgcctccg cctcttctat ctccgcctcc acctccacct
      541 ccacctgctc ttctatctcc gcctccgccg cctcttctat ctccgcctcc cgcgccgcgc
      601 ctgtctcccc tgtctccacc cccacgtctg tcgctaaatc cgcctcttct atcgccctgc
      661 atatcactat caggtttccc accacctgca attatatttg gattaaggtc cctcttagcg
      721 agaacttcac ccttacaaat ccatactttc actcctattt ttccataaac cgtttgtgca
      781 aatacatttg cataatcaat atccatacgt aatgtatgta atggagtacg gccttgtttt
      841 atttcttcgc tgcgtgcaat ttcagcccct cctaatcttc ctcctactct tactttaatt
      901 ccttcagcac ccattcttaa agcagaagca attgacattt taatcgctct tttataattg
      961 atacgatttt caatttgtct tgcaattgta tctgcaacaa tatttgcatc aagttcagga
     1021 cgacgtatct caagtatatt tatctggaca tcggccttac cggtaagttt ttttaattct
     1081 tctttaatgc gatcaacttc cccgccacct ttaccaatta taattcccgg cttcgatgtg
     1141 tgaatagtta taattaattt attaagtgta cgctcaataa caatctttgc tatcccgcct
     1201 ttgttaatac gggcatttag ataagttctt attttatcat cttcaatcaa ctttttcgca
     1261 tagtctttag ggtgagcaaa ccagttgcta tcccatccac ggattattcc taacctggca
     1321 ccaatcggat ttgttttttg acccatattc agtttttatt tttttgtttt ttttgctgca
     1381 gaattctttt cagttttttc ttcagcagaa ttttcttttt tttttgaggt tttagttgtt
     1441 gtttcttttt ttgcggttgc ttttttagta actgcttttt ttggcgcaac aactttctgt
     1501 tctgcctgta ccggaacttc ttctactatt ggttcagctt tagctaaagt cttttttact
     1561 gaaggaactc ctttatgatt tttactatca acaataatag taacatgatt gctgcgttta
     1621 cgaaccctat atccacgtcc ttgcggagcg gggcgcatac gtttaataac tcgtccgcca
     1681 tctaccatga tagtcttcac aataaggccg gcatcttcca ctttttcatc ggtattcttt
     1741 tgctcccagt tattaatagc gctttttaat aatttataca ttggcacagc cggatgcttg
     1801 ggattgaatt gcaaaatacc taaagctttt tcaacttcaa gtccacgaat caaatctgcc
     1861 agcaaacgca ttttgcgggg aga
//
LOCUS       DASVIM010000168         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_921434, whole genome shotgun sequence.
ACCESSION   DASVIM010000168 DASVIM010000000
VERSION     DASVIM010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921434"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..335
                     /locus_tag="VF305_03385"
     CDS             147..335
                     /locus_tag="VF305_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416208.1"
                     /translation="MPNIMPEGENIKKAIKWISASLEEKDNQLLPVLIEKALFKFDLS
                     PKDADFLTGFFGKHKTEK"
     gene            complement(465..1520)
                     /locus_tag="VF305_03390"
     CDS             complement(465..1520)
                     /locus_tag="VF305_03390"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7416209.1"
                     /translation="MMENNELAPVISRRSESTMSIILNRPSVLNSLNVEMIDIITGYL
                     ESAFADEKCRVILFYGSGNKGFCAGGDVKELAQKGKEKAYDEVSAFFKKEYALDLMIH
                     NSPKPIIVIADGITMGGGLGIAAGASIVIATERTRMGMPETKIGFFPDVGATGWMFTK
                     CPKGYPEYLGLTGYDMSGIECVRLGFATHFTNSKNIPKLIKTLEKYDFEKIADKELSQ
                     SIMGEISRFINFNIPTNQDMDEWVATYFSGKSDLNDILVSLTKCGLQQKLCDGVFASI
                     AERSPTALVLTLKLLRHNEGRPLPEVFAADFKAAMFLTSHHDYIEGIRARLIDRDDKP
                     RWIPDKIDQVDLTNFKL"
BASE COUNT          580 a          405 c          327 g          575 t
ORIGIN      
        1 cgaagtcaaa tatagcataa atgtaaattt tttaatcgaa cttataatgg cagtatccaa
       61 aaataaccaa aataggtata gttattgaat tacctatata tatagatcat gcagaaatat
      121 ttaattatca ggaggttccc atcattatgc ccaacataat gcccgaagga gaaaatataa
      181 aaaaagcgat taaatggatt tccgccagct tagaggaaaa agacaatcag cttttgccgg
      241 ttttgataga aaaagctctc ttcaaatttg acttgtctcc aaaagatgcg gattttttaa
      301 caggtttctt cggcaagcac aaaactgaaa aataaaattg aagctctccg cgacaagccg
      361 cggagaatct tcgattctta aggaataata tcattcagta ttcgctcact caccccgcag
      421 caaactacgg ggtttgcgct cgctgccgaa ttcaagaagc tgtattataa tttaaaatta
      481 gttagatcaa cctgatcaat tttatccggt atccaacggg gcttatcatc cctatcgata
      541 agcctcgccc ttataccttc gatataatca tgatggctgg tcaaaaacat agccgcttta
      601 aaatcggccg caaacacctc cggcaaaggt cttccctcat tatgccgcag taattttaaa
      661 gttaaaacaa gcgctgtcgg agacctctcg gcaatactgg caaaaactcc atcgcaaagt
      721 ttttgctgca agccgcattt ggttaaggaa actaaaatat catttaaatc tgattttcct
      781 gaaaaatacg ttgccaccca ctcatccata tcttgattgg tgggaatatt aaaatttata
      841 aatcttgata tttcccccat gatactttgt gacaattctt tatccgctat tttttcgaag
      901 tcatattttt ccaacgtttt aatcaattta ggaatatttt ttgaatttgt aaaatgcgta
      961 gcgaaaccaa gacgaacgca ttcgatcccc gacatatcat aaccggtgag acccaagtat
     1021 tccggataac ctttaggaca ttttgtaaac atccagccgg tcgcgcctac gtccgggaaa
     1081 aagccgattt ttgtttccgg catgcccatc cgtgtccgtt ctgtggctat aacgatactc
     1141 gcaccagcgg caatacccaa tcccccgccc attgtaatac cgtcggcaat tacaataatt
     1201 ggtttcggag aattatgaat catcagatca agtgcatatt cttttttaaa aaacgcgctt
     1261 acttcatcat atgctttttc ctttcctttt tgggcaagtt ctttgacatc tcctccggca
     1321 caaaatccct tattgccact accgtaaaaa aggatgaccc tgcatttttc gtcagcaaat
     1381 gcactctcca aatagccagt gataatatcg atcatttcta cattgaggct atttaagacg
     1441 ctgggacgat taaggattat tgacatcgtt gattctgaac gacgagaaat aaccggcgcc
     1501 aattcgttat tttccatcat tttcccacca aatcttagct atttgattta aagcttttta
     1561 aacgcatttg cgtctgaata aagaattata tatattttga ttataagcgt ttctattatc
     1621 ccggtcaatg ctcgcgtatt aatttgttcc actaattcgg ttaaagaatt taaggggatt
     1681 aaaaggcgac tggaaagtgg aaattcgcga tgctgccgga actttcctca aggatgttat
     1741 attcaaggtg gaataagtct gaacatagtt cttcccgcct cagacgaggc gggaagcgtt
     1801 ttttaaaact tattttcatc atccaggcag aagcaactat ttcttcagca aatctctaat
     1861 ctcgacaagt aacgactgat cttttgt
//
LOCUS       DASVIM010000169         6053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_240607, whole genome shotgun sequence.
ACCESSION   DASVIM010000169 DASVIM010000000
VERSION     DASVIM010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240607"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1822)
                     /locus_tag="VF305_03395"
     CDS             complement(233..1822)
                     /locus_tag="VF305_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7416210.1"
                     /translation="MMSKNKLPSDCDVIVIGAGVAGLTATALLTKSGLKVVTVEEQLK
                     PGGYFMGFKRKGFTFDSSIQWLNQCKDGGFIYNVFNFIGSDYPRCNNMNRIRRYKGES
                     TDYLLTCEPHKLRDQLIKDFPDETEGISKFFEDCKKNGNHFDILKNRMRAIETMSVFE
                     KMVLGIKMLWWVIPVWKILRISAEKGLDKYFKNEAIKKIFCCEEKFMSIIMPICWAYA
                     GDFQSPPKGGAEVFINWLCKKIEDTSSQIILNSKVEKVLIENKKIIGVTLADGHTIHS
                     KYVIAACDVENLYERMLPTGTVPGKLLNRLREADLYYSSVTLYIGLDCDASAIGIQEE
                     LTCVTVDNITRKEHFTGDPHKSSLIVQAPSVRDKTLAPKGKSTLTIQCAAWIDYKNFW
                     QTDKNLERGIAYKNFKQQFADILITRLEKVFNVNIKDHIEVLEIATPITYWRYTKNRD
                     GSIMGASPTDKNIKNKLAHYYTPVKNLFLGGHWAEYGGGMPIAVKAASNVSLLILKEI
                     KKQEFKKLRDAMDGRNKNIKT"
     gene            complement(2304..3584)
                     /locus_tag="VF305_03400"
     CDS             complement(2304..3584)
                     /locus_tag="VF305_03400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7416211.1"
                     /translation="MSFQKFELHEKILAGVQALGYKTPTPIQLQAIPPIMKGRDVMGL
                     AQTGTGKTAAFVLPILQRLLQTQRGGIRALIIAPTRELSEQTNEAIEQLGRKTKLRST
                     TIYGGVSINNQINRLRGNVEIISACPGRLLDHIERGTIDLSHVEVLVIDEADHMFDMG
                     FLPDVRRIVKHLPPKRQTLLFSATMPNDVRSLALDLLQDPINIQIGHSAPVMTVAHRF
                     YSVQMGIKTALLKDILEKTEMESVLVFTRTKSRAKSLAQQLDRSGHKVISLHGNLTQQ
                     KRKQALDGFRDGRYEIMVATDIAARGIDVTRISHVINYDMPSTADAYTHRIGRTGRAS
                     KTGDAFTFVTGDDIGLAHSLKRILGEKLQYHSVDGLTTEIQTSPSKQNTIDMKSNKRR
                     GSFARHTQTRRKPQQRSFQNANRSSWSHRSQSAA"
     gene            complement(3942..4667)
                     /locus_tag="VF305_03405"
     CDS             complement(3942..4667)
                     /locus_tag="VF305_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416212.1"
                     /translation="MTLLHNQHLVLMPGLDGTGLSFEPFIPLLPADAKITVVRYPIDK
                     LLSFEETVKCAAAQIPAGNPPIVIAESFSGPVAIQMIASGRIKAKALILCATCAKSPR
                     PIVWRIIRFLHLPLLIRPDMPRRFFKIIIGDDKLIAELMPLWKKVHAGVPAHVMDHRL
                     KIINRVDVTKWLKKFPVPCCYLQATNDRVIPSACLADFESHIPDLVIKRIKAPHFILQ
                     AQPQACLDAIEEFISLTSQLMQK"
     gene            4976..5611
                     /locus_tag="VF305_03410"
     CDS             4976..5611
                     /locus_tag="VF305_03410"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7416213.1"
                     /translation="MIKKEIDIEKLNKTAEKILIAARKTFAEYGYSGTHVDEIAQRAG
                     VNKATLYYQIGDKDTLYANVIHQVIGNVAQGIAQAVAKVDDPEEKLKAYIAFIADAVD
                     KNPEMPPIMMREIASGGLNLPRVVVEDISSVLTILISILDEGRKKGIFMEAVPFLIHM
                     MILGTILFYKGASPIKDRQVWLPADIKARDKKLKGSLGEEVARMVLKAIKK"
     gene            5617..5766
                     /locus_tag="VF305_03415"
     CDS             5617..5766
                     /locus_tag="VF305_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416214.1"
                     /translation="MRLEKIPGISRASIRKVRFVIPALHTPGKHPAGIQRETGCRFKS
                     GMTMI"
     gene            5807..>6053
                     /locus_tag="VF305_03420"
     CDS             5807..>6053
                     /locus_tag="VF305_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX7416215.1"
                     /translation="MKKKIIIAVFIALFVGVALFVYFGQKSNRAKELFYSGTIETTQA
                     KLSFQVPGRVAEVNVQEGQSVKKDQIVAQLDRVEFEAR"
BASE COUNT         1655 a         1260 c         1305 g         1833 t
ORIGIN      
        1 taccaacttt gataaaaatg caggttttca agtagtaatc attaatgctg ttttctcggt
       61 atttatgata ttgttttatg gttatgtgag ggctggagcc aacggatgta tggttgaaat
      121 aatgtggcac cgccgggaaa ccaggtggca acggagaaaa caaacatcaa cctgacgtag
      181 cgggcgaaac ctgtctgctc gactcaaaca agggaatttg cctcgcttaa atttaagttt
      241 taatgttttt atttctgcca tccatagcat cccgaagttt tttaaactcc tgttttttaa
      301 tttcctttaa aattaagagg ctcacatttg atgctgcttt tactgcaatt ggcattccgc
      361 cgccatattc agcccagtgc ccgcccagaa ataaattttt tactggagta tagtaatggg
      421 ccaatttatt ttttatattt ttatcagttg ggcttgcacc cataatgctt ccatcccgat
      481 ttttagtata tctccaatac gtgattgggg ttgcgatttc cagtacttcg atatgatcct
      541 ttatgttaac attgaacact ttttctaatc tggtaatcag aatatcagca aattgttgtt
      601 taaaattttt gtaggcaatt ccgcgttcta aatttttatc tgtctgccag aaatttttat
      661 aatcaatcca tgcggcacat tgaattgtta gcgtggattt ccctttcggg gcaagtgttt
      721 tatcccttac agaaggagcc tgaactatga gtgaactttt gtgcggatca cctgtaaaat
      781 gctcttttct tgttatatta tcaactgtaa cgcatgtaag ttcttcttgt attccgatag
      841 cagatgcatc acaatccagt ccaatatata aggtcacact tgaataatac agatctgctt
      901 ctctcaaacg attgagtaat ttacccggca ccgtaccggt gggcagcatt ctctcataaa
      961 ggttttctac atcgcatgcc gctattacgt atttggaatg aatagtatga ccatctgcga
     1021 gagttacacc aattattttt ttgttttcaa taagtacttt ttcgactttg ctatttagaa
     1081 ttatttgaga tgatgtgtct tctatttttt tacataacca gttaataaaa acttccgctc
     1141 ctccttttgg gggtgattga aaatctcctg cataagccca acaaattggc ataatgatgg
     1201 acatgaattt ttcttcgcag cagaatattt tttttatagc ttcatttttg aaatatttat
     1261 ccagtccttt ttctgcggat attcttaaaa ttttccatac aggtattacc caccagagca
     1321 tttttatccc caaaaccatc ttttcaaaaa ccgacatagt ctctatggca cgcatgcggt
     1381 tctttaaaat atcaaagtgg ttgccatttt ttttacagtc ttcaaaaaat tttgatattc
     1441 cttctgtttc atcaggaaaa tcttttatga gttgatctct gagtttatgt ggttcgcatg
     1501 tcagcagata gtcagtggat tctcctttat atctgcgtat gcggttcata ttattacatc
     1561 tggggtaatc agagccgata aagttaaaga cattataaat aaaaccacca tctttgcatt
     1621 gattgagcca ttgtattgag ctatcaaaag taaatccttt tctcttaaat cccataaaat
     1681 atccacccgg ctttaactgt tcctctactg tcactacttt aagaccggat tttgtcagaa
     1741 gggcagtggc ggtcaggccg gcaacaccgg ctccaataac tattacatca caatcagatg
     1801 gtaatttatt tttagacatc atattgggtt catccggtgc atgcttgctt ttgacttgaa
     1861 aagaatggac acacgactcc ataaagaaat tggaaacgat ttctttttag gggagggaaa
     1921 gtatgtccac gaaccttttg tatcacggtt ttgtctggtt ggctatgaat atgttcgcac
     1981 acgatgggaa tattggggac ggctgtaatt aattgacaac ttccgctaac ccacaccaat
     2041 cctagctttc cggccgtacc tgacgacatg attttttctt caaaggaatt atttgctggt
     2101 catagcgaag gtttgatatc actaaataat cattttaagg gtaataaact cttcaattgc
     2161 attgaggcac gtttgtggtt gcgattgcag aataaaatgc gcagctttaa tccattacct
     2221 ttttttggga cgagtggttt ggtctctata taggcgaata aacctgtaag catcgtatca
     2281 ggatacttac aggtttattt ttcttaagct gcagattgcg atcgatgtga ccacgatgac
     2341 cgattagcat tctggaagga acgctgctgc ggttttcttc tggtttgcgt atggcgggca
     2401 aatgatcctc ttcgcttgtt ggatttcatg tcaatcgtat tttgcttaga tggactggtc
     2461 tgtatttcgg tagttaatcc atccacgcta tggtattgca gtttttctcc gagtattctt
     2521 ttcagggaat gcgcgagacc aatgtcgtct cctgtcacaa atgtaaaagc gtcaccggtt
     2581 ttcgaggcgc gtcccgttct gccgatgcga tgagtataag catctgcggt agaaggcata
     2641 tcataattaa tgacatgtga tatccgtgtc acgtcaatac cgcgggcagc aatatcggtg
     2701 gcaaccatga tctcataacg gccgtcacgg aaaccatcca gcgcttgttt gcgcttctgc
     2761 tgggtgagat ttccatgcag cgaaatgact ttgtgtcccg atctatccag ctgctgggcc
     2821 aaactttttg cccttgattt tgtccgtgta aagaccagca ctgattccat ttcggtcttt
     2881 tcaagaatat ctttgagcaa tgctgtttta atgcccatct ggaccgaata aaatctatga
     2941 gcaaccgtca ttaccggtgc tgagtggccg atttgaatat tgattggatc ctgtagaaga
     3001 tccaaggcca ggcttcttac atcgttgggc atcgttgcgg aaaaaagcaa ggtttgacgc
     3061 tttgggggaa gatgcttgac gattcttcgg acatccggca gaaagcccat gtcaaacatg
     3121 tgatccgcct catcgattac cagcacctct acatgggaca aatcaatggt gccacgttcg
     3181 atgtgatcaa gtaaacggcc ggggcatgcg gagataattt ccacattacc gcgcagtcta
     3241 ttaatctggt tattaatgct tactccacca taaatagttg tactgcggag cttcgtttta
     3301 cgtcccagct gttcgatcgc ttcgttggtt tgctcgctca actcgcgcgt cggggctatg
     3361 atcagggcgc ggataccacc tcgctgcgtt tgcaggagac gttgtaaaat cggcagcaca
     3421 aaggctgcgg tttttcctgt tccggtctgg gccagtccca taacatcacg acctttcatg
     3481 atcggtggga ttgcctgtaa ttgaattggt gttggcgttt tgtaaccaag cgcttgcact
     3541 ccggcaagta ttttttcatg taactcaaac ttctgaaaac tcatttttta tcctttatct
     3601 tttgtattta ccagctattg gtatatttga acccatctcg aaccgttaag gctggtaaag
     3661 acaagacatt tcacacgctt gtttggcgtc tgaaatgcag gccacgaaca cggcattaaa
     3721 tttggattct atttagatta gcgggatttt cctgcgcaag tttctaaaaa gaaaggtggg
     3781 aaggcaaata actatcgcta tcaattgcgt actctttaca cgcataactg cccaatagca
     3841 aggaatatct ttataccaaa tcgccttcat tacttttaga aaatcaggca acaccgtact
     3901 tgtgccccct ctccttatcc taaaaagaat tatcacaaaa actatttttg cattaactga
     3961 cttgtgaggc tgataaactc ttcaatcgca tcaaggcagg cctgcggttg ggcctgaaga
     4021 ataaaatgcg gagccttaat cctttttatt actaaatcgg gaatgtggct ttcaaaatcc
     4081 gccaggcagg cagatggaat tacccgatcg tttgtcgcct gcaagtaaca acagggtaca
     4141 ggaaactttt tcagccactt tgttacatct actcgattga tgattttgag ccgatgatcc
     4201 atgacatgcg ccggaacgcc agcatgcacc ttcttccata gaggcataag ctcggcaatg
     4261 agcttatcat ctccgatgat gattttaaaa aaacgcctgg gcatatctgg tctgatgagc
     4321 aagggtaggt gcaaaaaacg tataatacgc cacacaatcg ggcgcggaga cttggcgcaa
     4381 gtagcgcaca gaataagagc ttttgccttg atgcggccgg aggcgatcat ttgaatggcg
     4441 acaggtcccg aaaaagattc ggcgataacg atgggaggat tgcccgcggg aatttgcgct
     4501 gccgcacatt tcacagtttc ttcaaacgat aggagtttat cgattggata gcggacaacc
     4561 gtaatcttcg cgtccgccgg aagaaggggt atgaatggtt caaaagataa tcccgtgccg
     4621 tccagaccgg gcataagaac tagatgttga ttgtgtagca gtgtcattat tttgcctcgg
     4681 attaaataca tatactttat aactttgtat tgcaataacc atgcggcaaa atccgcttga
     4741 cattttccgc caaatattta actatttggt taaaccagac ggttaaatga caacttcgta
     4801 aagaatctta tatgctgcgt gacgcttcat ctttccccgg cttcgccggg gcaggctacc
     4861 tgcgatccgc cacggcggac ctcattcctc gtgattcgtg tgcctggcct ctgaaaattt
     4921 ttacgaagtc gtcgtatata tactgggtgg aaatgacaaa acatgtgggt aaaaattgat
     4981 caaaaaagaa attgatatag aaaaacttaa taagaccgcc gagaaaattt taattgcggc
     5041 gcggaagact tttgccgagt atggttacag cggaacgcat gtggatgaaa ttgcgcagcg
     5101 cgccggtgtg aacaaagcaa ctctctatta tcaaatcggc gataaagata ccctctacgc
     5161 taatgtcatc catcaggtta ttggtaatgt cgctcagggt attgcccagg ctgtagccaa
     5221 agtcgatgat ccggaagaaa aactcaaagc atatatagca tttattgccg atgccgtaga
     5281 caaaaatccc gaaatgccgc cgatcatgat gcgggagatt gcctccggcg gcttaaactt
     5341 accgcgagta gtcgtagaag acatatcttc ggttttaact atcttgataa gtattctcga
     5401 cgaaggaaga aagaaaggga tattcatgga agcggttcct ttccttattc acatgatgat
     5461 tttaggaact attctttttt ataaaggagc ctctccgatc aaagaccggc aagtatggtt
     5521 gcctgccgat atcaaagccc gcgacaaaaa attaaaaggc agtctgggtg aagaggtcgc
     5581 caggatggtg ttgaaggcaa taaaaaagta gagtggatga gactggaaaa aattcccggc
     5641 ataagccggg caagtatacg caaagtacgt tttgtcattc cggccctgca tacgccaggt
     5701 aaacatccag caggaatcca gagagaaact ggatgccggt tcaagtccgg catgacgatg
     5761 atttaaaatt tattgcagga ttaataatta aaagggagaa ttagtagtga aaaagaaaat
     5821 tatcattgcc gtatttatag ctctctttgt aggtgtggcg ctgtttgttt acttcggtca
     5881 aaaaagcaat cgtgcaaaag agctttttta ttccggcaca attgaaacga cacaggctaa
     5941 attgtctttc caggtacccg gacgcgtcgc tgaagtcaat gttcaggaag ggcaaagcgt
     6001 taaaaaagat cagattgtcg cccaactcga ccgcgtggaa tttgaagcgc gtt
//
LOCUS       DASVIM010000170         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_495256, whole genome shotgun sequence.
ACCESSION   DASVIM010000170 DASVIM010000000
VERSION     DASVIM010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495256"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VF305_03425"
     CDS             <1..1329
                     /locus_tag="VF305_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7416216.1"
                     /translation="FMKKLINKWPFLVIILLGLFALVGPYHDSRVSALDKSVYKDIKT
                     FSEVFDMVKKNYVDEVDSATLIQGAINGMIRSLDPHSAFMTPEIYKELEVETQGRFGG
                     IGIEITIVKDVLTVVSPIEDTPAFIAGIKTGDQIIRIDGKSTKDITIMEAVKKLRGPK
                     DTKVTITIMRENMAKPKDFILTRAIIQVKSVKAKTFDDNIGYIRIASFHERTSDDLHK
                     ALREVSEKINPMKGLVLDLRNDPGGLLIQAIEVSDMFLKSGVIVSTRGRTKNMETKTM
                     ARNDDNEISCPIVVLVNEGTASAAEIVAGALQDNGRALVIGTQTFGKASVQTVIPLED
                     GSALKLTTARYYTPKGRSIQAEGIKPDIVVKYVRPSEDAENNTAWWEDRVREKDLKGH
                     IKPAKEDGTKLDENQAKDVKDPSDLAHDNQLKTAIDILKSWDIMKKNLGS"
     gene            1364..2323
                     /locus_tag="VF305_03430"
     CDS             1364..2323
                     /locus_tag="VF305_03430"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEX7416217.1"
                     /translation="MRLFKRLFTVSVIFLIVVLIAAIIYVLQFKEEPSVIKEPEKQTK
                     QIEPQKTITPKEGKAKLVKEKPLPLKTAPEKEKKKIVISKIGKVPLPLRQVAIIIDDI
                     GYDLGPVKELLKINADLTFAILPLCTHTREAAEMLHRAQRETLLHLPMEPDSYPREKP
                     GNGALFTDMNDEELVFQLEKDLAAVPYISGVNNHMGSKFMGDEKKLSLIFSKLKKKNL
                     FFIDSRTSANTKAFAAAEKVGLPVAARKIFLDNSRDYNEIYNNLINITKNNGDVSPMI
                     IIGHPYPETIQAINDAIKVMQEKGVSIVPVSQIIKAKASNRTS"
     gene            2375..>3194
                     /locus_tag="VF305_03435"
     CDS             2375..>3194
                     /locus_tag="VF305_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7416218.1"
                     /translation="MQKKRTYLVFITILVFINLCGCSYLPAVRVPSASSEESSADATF
                     HYSLGVLLRLDGKIEEATSELEQAVAADPGSSYLTNELVSLYTEKGDLHRAISLGEET
                     LSKNPDNIELHLIMGGLYLNLREYRKAIKEHEKVIELDPKNLIAHLYLAMIYAEEKNF
                     DKSEETFKKMLKIDPDNVMGIYYYGKVLAEMKRFEDAEKMYKKAMSLKPYFESVLFDL
                     AALYEKQKKLDSAIEVYRNYVEINPARINLRVKIGELLIKEKKYEQAQKEFQEVL"
BASE COUNT         1056 a          567 c          707 g          864 t
ORIGIN      
        1 tttatgaaaa aattaataaa taagtggccg tttttagtta ttattctcct cgggttattt
       61 gccctggtag ggccttatca tgatagtagg gtttccgcgc tggataaaag tgtctataaa
      121 gacattaaga cgttcagcga agttttcgac atggtaaaga aaaactatgt tgacgaggtg
      181 gattcagcaa cgttaattca aggcgcaatc aacggaatga tcaggtcact ggaccctcac
      241 agcgcgttta tgacgccgga gatatataaa gagttggaag tggagacaca ggggcgtttc
      301 ggaggcattg gtattgaaat tacaatagta aaggatgttt tgaccgtcgt ttcccctatt
      361 gaagatactc ccgcatttat tgccggaata aaaacgggcg accaaattat aagaatcgat
      421 ggcaagtcca caaaagatat taccatcatg gaagcagtaa aaaaattgcg cgggcctaag
      481 gacacaaagg tcacgatcac gattatgcgg gaaaatatgg caaaacccaa ggatttcatc
      541 ctgactcgtg ctataattca ggtcaagagt gttaaagcca agacctttga cgataacatc
      601 ggttatatcc ggatagcttc tttccatgag aggacgtccg atgatttgca caaagcgttg
      661 cgggaagtta gcgaaaagat taatccgatg aagggattgg ttctggattt gagaaatgat
      721 cctggtggat tgttgattca agcaatcgaa gtctctgata tgttccttaa atcgggagtt
      781 attgtttcta ctcgcggacg aacaaaaaac atggagacaa agacaatggc gagaaatgac
      841 gataatgaaa taagttgtcc tattgttgtt ttggttaatg aaggtacagc cagcgccgcg
      901 gagattgttg ctggagccct gcaggacaat ggccgggctt tggttatcgg aacgcaaact
      961 tttggtaaag catcggtgca gaccgttatc ccgctggaag atggttccgc gcttaagctg
     1021 acgaccgcac gttattacac gccaaaggga cgatcaattc aggcggaagg catcaagccg
     1081 gatattgttg ttaaatacgt aaggccgtcg gaagatgcgg aaaataatac tgcctggtgg
     1141 gaagaccggg ttagagaaaa agatctcaaa ggtcatataa aaccggctaa agaagatggc
     1201 accaaacttg atgaaaatca ggccaaggat gtaaaggatc cttctgattt agcccacgat
     1261 aatcaactca agacggcaat tgatatctta aagagctggg atatcatgaa aaagaatctg
     1321 ggaagctaaa ttgtttatag aaagtgcaaa attatagtta tttatgagat tatttaaacg
     1381 cctttttacg gtatcggtaa tatttttaat cgttgtatta attgctgcca tcatttatgt
     1441 gctgcaattt aaagaagaac catcggtaat taaagagccg gagaagcaga caaaacaaat
     1501 agaaccgcag aaaacaatta cacctaaaga gggcaaagct aagcttgtaa aagaaaaacc
     1561 cttgccctta aagactgcac cggaaaaaga aaagaaaaaa atagttattt ccaaaattgg
     1621 aaaagttccc ctgccgctaa gacaagtagc aattattatt gacgacattg gttatgacct
     1681 ggggccggta aaagaattac tgaaaattaa cgccgattta acatttgcta ttttgcctct
     1741 ctgcactcat acccgcgagg cagcggaaat gcttcatcga gctcagcggg aaacactttt
     1801 acatctgcct atggagccag attcttatcc gcgtgaaaag ccgggtaacg gagctctttt
     1861 tacggatatg aatgatgaag aactagtatt tcagttggaa aaagatcttg cggctgtgcc
     1921 ttacatctct ggggtcaata accatatggg ttcaaaattc atgggggatg aaaaaaagct
     1981 tagtttaatt tttagtaagt taaagaaaaa gaatttgttt tttatagatt cgcgcacgtc
     2041 agctaatact aaagcttttg ctgctgccga aaaagttggc ttgccggtag ctgcgcgtaa
     2101 aatattcctt gataatagtc gagactacaa tgaaatttat aataatttga ttaatattac
     2161 taaaaataac ggcgatgttt caccgatgat tattattggc catccttatc cggaaacaat
     2221 tcaggcaatc aacgatgcta ttaaagttat gcaggaaaaa ggagtatcga ttgttccggt
     2281 atcgcagata attaaagcaa aagcctccaa tagaacttca taattttgtc agaaaaaaag
     2341 gaaaaataaa attaaacaaa gctggttgtt cactatgcaa aaaaaacgaa cttatctagt
     2401 attcataacg attttagttt ttattaattt atgcggctgc agttatttgc cggcggttcg
     2461 tgttccctct gcatcttctg aagaaagttc agcagatgcg acctttcact attccttggg
     2521 tgttctttta cgtctggacg gcaaaattga ggaagcaact tctgagttgg aacaggcagt
     2581 tgccgcagat cccggctctt cttatcttac gaatgaactt gtttctcttt atacggaaaa
     2641 gggcgatctc catcgcgcca tttctctggg tgaagaaact ctttctaaaa atcctgataa
     2701 tatcgaactc catctgatta tgggcggttt ataccttaat ctccgcgaat atagaaaggc
     2761 catcaaagaa cacgaaaaag taattgaact ggatcctaaa aatcttattg cccaccttta
     2821 tttagcgatg atatacgccg aagaaaagaa ttttgataaa tctgaagaaa cctttaaaaa
     2881 aatgctgaaa attgatcctg acaatgtcat gggcatctac tattacggca aagtattggc
     2941 ggaaatgaag cggtttgaag atgcagagaa aatgtacaaa aaagctatgt ctttaaagcc
     3001 ttattttgaa tcggtcttgt ttgacttggc cgcgctctat gaaaagcaaa agaagctgga
     3061 ctcggccatt gaagtttacc gcaattacgt ggagatcaat cctgcacgga taaatttgcg
     3121 agttaaaata ggagaacttc taattaaaga aaaaaaatat gagcaagcgc aaaaggaatt
     3181 ccaagaagta ttga
//
LOCUS       DASVIM010000171         3618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_495398, whole genome shotgun sequence.
ACCESSION   DASVIM010000171 DASVIM010000000
VERSION     DASVIM010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495398"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..619)
                     /gene="dut"
                     /locus_tag="VF305_03440"
     CDS             complement(176..619)
                     /gene="dut"
                     /locus_tag="VF305_03440"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX7416219.1"
                     /translation="MEKIKVCIQKLDGNNDIPLPRYMTNQAAGMDIFAAVTEEEIILP
                     GQRKKIPTGIKIALPKGYEAQIRPRSGLAINQGITLLNSPGTIDADYRGEIALIAINL
                     GEEPFVVKRGMRLAQMVVQKIFQVDWVETAELTNTSRGNGGFGHT"
     gene            complement(749..2869)
                     /locus_tag="VF305_03445"
     CDS             complement(749..2869)
                     /locus_tag="VF305_03445"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX7416220.1"
                     /translation="MSNIFSADFAGRNISLKTNYVAGQADGSMLIYYGDTVVLVTAVS
                     LKSAREGVDFLPLTVDYQEKTFAAGKIPGGFFKREGRPNEREILTSRIIDRAIRPLFP
                     KGYFYETQVVAMVLSVDKENDSDVTAMIGASATLEMSNIPFKGPIAGVRVGRINGEFV
                     CNASADKMQESELNIFLVGRKVTPSKSGKPYDVELVMMEGEAKEVEESIIIEAIKFGL
                     EAIRPAIDVQDKMREAVGKEKRPLEKVVPDEELVARVSAEALAGLKEGYSMPRKLERY
                     GKLGSVREAVIKNIGGDDALLCKKIAAIVEELERRILRDMIIQNKKRIDGRSSTDIRP
                     ISAEVGVLPRAHGSALFNRGETQALVALTLGTSSDAQRMDYIGGEEMRSFLLHYNFPP
                     YCVGEAKPLRSPGRREIGHGALARKALVPVLPSSEVFPYTIRIVSEILSSNGSSSMAT
                     VCGGILSLMDAGVPVKDIVAGIAMGLLKEGDKVVILSDILGDEDHAGDMDFKVCGTEK
                     GVTAMQIDIKIDGLTEDILSKALTQAKEGRIFIIGKLRQTIAAPRPDISLYAPRITTV
                     KVKEDQVRNVIGSGGKNIRQIISETGVTIDVEDDGTVTIASSDAEAAARAVAMVKWLT
                     EEAEVGKIYRGTVKKIVDFGAFVEILPGTEGLLHISQIAKERINKVTDVLKEGDEVMV
                     KVLEMDKQGKIRLSRKDALGAEVK"
     gene            complement(3005..3271)
                     /gene="rpsO"
                     /locus_tag="VF305_03450"
     CDS             complement(3005..3271)
                     /gene="rpsO"
                     /locus_tag="VF305_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX7416221.1"
                     /translation="MLTLEKRKTLIADYRLHEKDTGSPEVQIALLSARIEYLTEHFKA
                     HKKDHHSRRGLLKLVGQRRRLLNYIKDTDVERYRNIIKRLGLRK"
     gene            complement(3319..>3618)
                     /locus_tag="VF305_03455"
     CDS             complement(3319..>3618)
                     /locus_tag="VF305_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416222.1"
                     /translation="KCELLAKMLPMTKSLPLLTGIEVEEDFANKLRDGMQPSVETMQL
                     HVLPFLEAGDMIKFVSRDGSLVAVAEMVAPVNKFSEFDGKRQAAKIIRVFNNISN"
BASE COUNT          921 a          913 c          669 g         1115 t
ORIGIN      
        1 gacagcacgc ccatctttga ttttttcgtt aatttcctga aatgtttttt tgactttgaa
       61 ttttttcatg ctcgaacttt ttcttatccc gtatatagtt acatcatttt catatcattg
      121 tttaaggcca attgcaaaat caaagacgat tatacgcata cggttaagct taattttagg
      181 tatggccaaa tccgccgttt cctctggacg tattggttaa ttcggcggtt tcgacccaat
      241 ccacttgaaa aattttctgt acgaccattt gcgctaaacg catgcccctt ttaacaacaa
      301 agggctcttc tccaagattg atggcaatca aggcaatttc tccgcgataa tcagcatcga
      361 ttgtgcccgg cgaattgagc aatgttatcc cctgatttat agccagtccg ctacgaggtc
      421 ttatttgcgc ttcgtaacct ttgggtaagg ctatcttaat gcctgtgggg atttttttcc
      481 gttgaccggg cagaataatc tcttcttcag ttacagccgc aaatatatcc attccggcag
      541 cctgattagt catgtatcga ggcaaaggga tgtcgttatt accatctagt ttttgaatgc
      601 atacctttat tttttccatt atttttccaa ttaaaaatca agttagcaac atacaaacat
      661 ttttttacgg tgagaactgt tctgtatcca tttagttatg gacacagaac agctgttttt
      721 gaaaggttat cgttaatctc gcttcaactt atttgacttc cgcccctagt gcatctttcc
      781 ggctaagcct tatcttgccc tgcttgtcca tttccagaac tttcaccatt acttcatcgc
      841 cttcttttaa tacatcggta accttattga ttctttcttt ggcgatttgc gatatatgca
      901 gcaagccttc ggtcccgggt aaaatctcta caaaggcacc gaaatcaaca attttcttta
      961 ctgtgccacg gtaaattttc ccgacttcag cttcctcggt gagccatttg accatagcta
     1021 cagcgcgggc tgccgcttcg gcatcgctgg aagcaattgt taccgtgccg tcatcttcaa
     1081 cgtcaatggt gacccctgtt tcactaataa tctgccggat attcttaccc cctgacccaa
     1141 taacattgcg tacctggtct tccttgactt tgaccgttgt aatacgagga gcgtaaagag
     1201 aaatatccgg acgcggcgcc gctatcgtct gccgcaattt accaattata aaaattcggc
     1261 cttcttttgc ttgggtcaga gctttgctta aaatatcttc ggtcagacca tcaattttaa
     1321 tgtctatttg catggccgta acgccttttt cagttccaca gaccttgaaa tccatatcac
     1381 cggcatgatc ctcgtcacca aggatgtccg ataaaatgac gactttatcc ccttctttta
     1441 aaagacccat ggcaatgccc gcaacaatgt ctttcaccgg cacaccggca tccattaagg
     1501 agagaattcc gccgcaaacc gtagccattg aagacgaacc attagatgaa agaatttcgg
     1561 atacaatcct gatggtgtag ggaaatactt cggaagaagg caaaaccggt accaaggctt
     1621 tcctcgccaa agcgccgtgg ccaatttctc ttctgccggg gctgcgcaaa ggtttggctt
     1681 cgcccacaca atagggcggg aaattgtaat gtaaaaggaa tgacctcatt tcttcgccgc
     1741 ctatataatc catgcgctgt gcatcagaag atgttcccaa agtaagggca accaacgctt
     1801 gcgtctctcc gcgattaaat aatgcggacc catgggcgcg aggcaaaact cctacttcgg
     1861 cgctaatcgg acgaatatca gtagatgatc taccatcaat tcttttctta ttttggatga
     1921 tcatgtcgcg caaaatgcgc ctctccaact cttcaacaat tgcggcaatt ttcttacaca
     1981 gcaaagcatc atcgccgccg atattcttga tcacggcttc ccttacggaa ccaagtttgc
     2041 cgtagcgttc caatttacgg ggcatgctat agccttcttt taggccagcc aaagcctcgg
     2101 cgctaacgcg agcaacaagt tcttcatcag gaaccacttt ctctagcggc cttttttctt
     2161 tgccgactgc ttcgcgcatt ttgtcctgta catcgattgc cggacgaatt gcttccaaac
     2221 caaatttgat ggcctcaatt attatacttt cttctacctc tttggcctcg ccttccatca
     2281 tgaccaattc aacatcataa ggtttaccgg atttacttgg cgttactttt ctgcccacca
     2341 ggaaaatatt caattcgctt tcctgcattt tatccgccga ggcattgcag acgaactcgc
     2401 cattaattct ccccacgcgc acgccggcaa tcggcccctt aaacgggata ttggacattt
     2461 ccaaagtggc tgatgctcca atcatggccg taacatcaga atcattttcc ttgtccacgg
     2521 aaagcaccat agccactact tgtgtttcat aaaaatatcc tttggggaac aaaggacgta
     2581 ttgcccggtc gatgatgcgg gatgttaaaa tttcgcgttc attggggcgt ccttctcttt
     2641 taaaaaatcc tcccggaatt ttcccggcag caaaagtctt ttcctgataa tctacagtta
     2701 aaggcagaaa atcgactcct tctcttgcac tctttaaaga tactgccgtc actaaaacaa
     2761 ctgtatcgcc ataataaatc agcattgaac catcagcctg accggcaacg taattcgttt
     2821 tcagggaaat attgcgaccg gcaaaatccg cactaaatat attactcatt tacttttcct
     2881 ccacattctt tctaggtgct ggcaacaaga agttcaaggg cgaaacgcaa tagcctactt
     2941 ccgtccgcag ataaatttac gcagccaatt agtaaactat tgccttccgc ccttgaatta
     3001 ttatttattt cctgagtccc agacgtttaa ttatatttct gtatcgctcg acgtcagtat
     3061 ctttaatgta attaagaagt ctccttcttt gaccgactaa tttcagcagg ccgcggcgtg
     3121 aatgatgatc ctttttatgc gctttaaagt gctccgtcag atattctatt ctggcgctca
     3181 aaagggcaat ctggacttcg ggtgagcccg tatccttttc atgaagacga taatctgcaa
     3241 ttaatgtttt cctcttttcc aaagttaaca cactattccc tccctataat taattcgttg
     3301 tttgttttaa attttctttc aattactaat attgttaaac accctgataa ttttggctgc
     3361 ctgccttttt ccatcgaatt cagaaaattt attcaccggc gccaccattt ccgccacagc
     3421 aaccagtgaa ccatcgcggc tgacaaactt tatcatatct ccggcttcaa gaaaaggaag
     3481 aacatgcagt tgcatagttt ccacggaagg ctgcattccg tctcgtaatt tattggcaaa
     3541 gtcttcttcg acctcaattc cagtcaaaag cggcagtgat tttgtcatcg gcagcatctt
     3601 tgccagcagt tcgcattt
//
LOCUS       DASVIM010000172         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1690771, whole genome shotgun sequence.
ACCESSION   DASVIM010000172 DASVIM010000000
VERSION     DASVIM010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690771"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..574
                     /locus_tag="VF305_03460"
     CDS             77..574
                     /locus_tag="VF305_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416223.1"
                     /translation="MTKLNKRQIIILVIAALVFLYAVYEYLIAGPASKKVKTSADPAE
                     INTFVSDLTSNLIKDNLAAVDTYIVGRAEADWQKNPFWERESYKAWAAREGAAGGSGA
                     SAKIIYSGYVDSGNKKMAVINGLEYRIGEQLEMEGYVLKSITPSKVLIVNKNTGSEVE
                     VSIQE"
     gene            599..1498
                     /locus_tag="VF305_03465"
     CDS             599..1498
                     /locus_tag="VF305_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX7416224.1"
                     /translation="MKCSKSNKDIVMLGFSFFVLIFFLGCADGLNSTKKDPFFDKWTT
                     QAENSQGHSPSAEPKNINVADLTAKQSSSAALAGSAMKKLPSKKINLTMRQAELKTVL
                     RAMAKSVDLNLMVKNDLKGEISVDFRGVPWDQAFTGLLRTYGLSYVWEGNIILVKTVE
                     DIEQDLKQKMQLRDIQWVEPLLQPVVVNIDYADPKKLMETLQDLLTKDKDGKPRGSVK
                     VDEHTNSLVISAMSNDLEKMLRIIEKLDRPTPQILIKANIVETTKEIARDLGIMWGGE
                     AEYRRLYRHGRRHACELTANDSS"
BASE COUNT          477 a          277 c          395 g          396 t
ORIGIN      
        1 cagcagcatc ccgattccat ggaatttaga atgaaaattt ggatagccct gggcagttaa
       61 tatacataag gtatttatga ctaagttaaa caaaagacaa attatcattt tagtaattgc
      121 agctttggtt tttttatatg cggtttatga atacttaatt gccggcccag cgagtaaaaa
      181 agttaaaacc agcgccgatc ctgcggaaat taacactttt gtgagcgatc tgaccagtaa
      241 tttaataaaa gacaatctcg ctgcagttga cacatacata gtcgggcggg cggaagccga
      301 ttggcaaaaa aatcctttct gggaaaggga atcatataag gcatgggcgg ccagagaagg
      361 ggcagccggc ggaagcggtg catcggctaa aataatttat tccggttatg ttgattccgg
      421 taataagaaa atggccgtca ttaacggttt ggaatatagg ataggggagc aattggaaat
      481 ggaaggctat gtattaaaaa gtattacgcc ttcgaaggtt ttgattgtca ataaaaatac
      541 gggaagcgaa gtggaagttt ccatacaaga ataaaattta gcattggagg ttgtcgactt
      601 gaaatgctct aaaagtaata aagacatcgt aatgttgggt tttagttttt tcgtgctgat
      661 atttttctta ggctgtgccg atgggctaaa tagcacgaaa aaagatcctt tttttgataa
      721 atggacaaca caggcggaga attctcaagg ccactctcct tctgccgagc ctaaaaatat
      781 taatgtagcg gatttaacgg caaagcaatc ttcttccgcg gctttggcgg gatcggcgat
      841 gaaaaaactg ccttccaaga aaattaatct caccatgcgc caggcggagc taaagaccgt
      901 gctgagggca atggccaaat cggttgattt gaaccttatg gtaaaaaatg accttaaagg
      961 tgaaatcagt gttgatttcc ggggcgttcc ctgggaccag gcctttaccg gattattgcg
     1021 cacttatggt ttatcttatg tctgggaggg caatataatt ctggtgaaga ctgtcgaaga
     1081 tatcgaacag gatttaaaac aaaaaatgca attgcgggat attcaatggg tggagccgct
     1141 tttacagccg gtagtggtaa acatcgatta cgccgatccc aagaaattaa tggagacgct
     1201 gcaggatttg ctgacgaaag acaaggatgg aaaaccgcgc ggttcggtta aagttgacga
     1261 gcacaccaac tctctggtta tttccgccat gagtaatgat ctggagaaga tgctgcggat
     1321 tattgaaaaa ttagatagac caacgcccca gattttaatt aaagccaata tagtggaaac
     1381 aacgaaggaa attgcccggg atttaggaat tatgtggggg ggggaagcag aatacaggcg
     1441 attataccgc catgggcgga ggcacgcgtg cgagctcact gccaacgact ccagttaatc
     1501 cgacagcagg tgggcccgtt gcaatgggag gatcgtcagg atttg
//
LOCUS       DASVIM010000173         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_922434, whole genome shotgun sequence.
ACCESSION   DASVIM010000173 DASVIM010000000
VERSION     DASVIM010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922434"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /gene="purB"
                     /locus_tag="VF305_03470"
     CDS             complement(<1..974)
                     /gene="purB"
                     /locus_tag="VF305_03470"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX7416225.1"
                     /translation="MTKIWSPENRYQKWLDVEIMACEAMAKLGKIPESALKNIKEKVS
                     INVKRIDEIEKTTKHDVIAFLTSLTEKVGEDGRFIHMGLTSSDILDTSLAILLKESAE
                     ILLEDIDQLLVVLKKKALEHKNTLMIGRSHGIHAEPVTFGLKMALWYQEMQRNHRRLV
                     NAKESVSYGKISGAVGTFSFVEPFVEEYVCRKLALKPAPVSSQIVQRDRHAEFFTTLA
                     IIASSVDKFAQEIRLLQRTEVREAEEYFSAGQKGSSAMPHKRNPVLSENLSGLARLVR
                     SYALAALENVALWHERDISHSSVERVIGPDATIVLDFMLGGFTGMMDKL"
     gene            1198..1860
                     /locus_tag="VF305_03475"
     CDS             1198..1860
                     /locus_tag="VF305_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX7416226.1"
                     /translation="MSKAIAETGKYSLIEEIVNSIIHGTGILISLAGLVLLIVFSSLY
                     GKASHIVSSTIFGITLVLLYTASTLYHSFREPQIKHVFRIIDHSCIYALIAGTYTPFM
                     LVVVRGVLGWTMFAIVWSLTILGILFKIFFIHRFKIFSTLAYILMGWLVVFAIKPIFQ
                     TLPGGAIVWLVSGGLAYTGGTIFYAWKKLPFNHAIWHLFVLTGSICHFFAVLFYVVPL
                     KT"
     gene            2160..>3079
                     /locus_tag="VF305_03480"
     CDS             2160..>3079
                     /locus_tag="VF305_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX7416227.1"
                     /translation="MKKYLQLIFCLTIILLATGSLWAKDKYTVTVLPFSLHSAENIDY
                     VKQGIGDMLSTRISVPNKIEVTGKDVVLTELKKYKTKEMTLVEVYDLGKRLKSDYVVW
                     GSISKIGSSISIDGKLVDIAAGKADIGIFTQSQSLDEVIPKINDFSQRIVQHILGVTP
                     PSAPAVAAPAPTVTRPTAPGLSRESQIITGMKTGKRGTLTSAINTEFINAPDPLNRRG
                     FWMSQKLPTEFKGMDIGDVNNDGLNEVVVIDSHNVYIYQKTANELKLLEKIAGKSSDN
                     YIAVDVADINRDGVKQIFVTSLNHTTLDSFV"
BASE COUNT          904 a          640 c          570 g          965 t
ORIGIN      
        1 agcttatcca tcatcccggt aaaccctccc aacataaaat ccaaaacaat tgtagcatcc
       61 ggtccgataa ctctttctac tgaagaatga cttatatccc tttcatgcca aagggctaca
      121 ttttccagtg cggccaacgc gtaagatctg accagcctgg ctaaaccgga aagattttct
      181 gaaaggacgg gattgcgttt atgcggcata gccgatgaac ccttttgccc cgcggaaaaa
      241 tattcttccg cctctcgaac ctccgtccgc tgaagcaacc tgatctcctg cgcaaattta
      301 tccacggaag aggcgattat cgccagagtg gtaaaaaatt cggcgtgtcg atcacgctga
      361 acaatttgcg atgataccgg tgccggtttc aaagcaagtt tgcggcatac atattcttcc
      421 acaaatggct ccacaaaaga aaaagtgcct accgctcccg atattttccc gtagcttacg
      481 gattctttgg cattaaccag acgccggtga ttgcgctgca tttcctgata ccacaaggcc
      541 atcttcaacc cgaaagtaac cggctcggcg tgaatgccgt gcgaacggcc gatcatcaag
      601 gtatttttat gttccagagc tttctttttt aagacaacta aaagttgatc gatatcttcc
      661 agcaaaattt ccgccgattc tttcaacaag atggcgagcg atgtatccaa aatatcggaa
      721 gaagtaagac ccatgtgaat gaaacggccg tcttcaccta ctttttccgt taaagaggtc
      781 aaaaaagcta tgacatcgtg ctttgtcgtt ttttctattt catcaatacg tttgacatta
      841 atagaaactt tctcttttat atttttgagc gcactttccg gaattttgcc cagcttagcc
      901 atcgcttcgc acgccataat ttccacatca agccactttt gatatctatt ttccggactc
      961 caaatttttg tcattttttc tcgcgaatat ctggaaatca cttactcctc ctcaagaata
     1021 tttatagtta tcgcaagttg gttaactttc ttctctccat ttttttgtag attgatatta
     1081 ttatgatttt gattagtacg tcaagaccta ttaataatcc ggccggtcaa ttaaaaattg
     1141 acaaattata cacgaaagca tattttaata aaataaaatt ggaagacggg tgactgcatg
     1201 tccaaagcta tagcggaaac tggaaaatac agtctgatag aagaaattgt taatagcatt
     1261 attcacggga ccgggattct aatcagcctg gccggacttg ttcttttaat cgttttttcc
     1321 agcttgtacg gtaaggccag ccatattgtc agttctacaa tttttggtat tacattagtc
     1381 ttactttaca cggcttcaac actctatcat agtttccgag aaccacaaat taaacacgtc
     1441 tttagaatta tcgaccattc ttgtatttac gctttaattg cgggaactta tactcctttt
     1501 atgctggtcg tggttcgcgg ggttttgggc tggactatgt ttgctatcgt ttggtctctg
     1561 accattttgg gaattttatt taaaattttt tttattcatc ggtttaaaat tttttccacg
     1621 cttgcttata ttctaatggg ctggctggtt gtttttgcca tcaagcctat ttttcagaca
     1681 ctccccggag gcgctattgt ctggcttgtt tccggcggac tagcttatac ggggggcact
     1741 atcttttatg cctggaaaaa acttcccttt aatcatgcca tctggcattt atttgtactt
     1801 accggcagta tctgccattt tttcgctgtt ctcttttacg tcgtaccatt gaaaacttaa
     1861 ataaagcaca tacaaatgat tctaaaaaga agttactttt tttacgccga cgtttttgcg
     1921 aaaataggat caaagctgaa agtttataaa agctgcctta tcctgattta ataaagacag
     1981 gaagataacg cttatcgact aatgttcaac ttttcgaaag ctcaatcatt tattaccgtt
     2041 tctataaaga acaaataaca aattgaaatt gaacttccta aatcattctt gtattatcat
     2101 ctttaaaatg ttagaagttt atggattcaa acgttagcat tccgaacagg gagataaata
     2161 tgaaaaaata tttgcaatta atattttgtt tgacaatcat tttgctggct accggttcac
     2221 tttgggcaaa agataaatac actgtaacag ttttaccgtt ttctttacat agtgccgaaa
     2281 atatcgatta tgtaaaacag ggaatcgggg atatgctctc tacacgtatt tccgttccta
     2341 ataaaattga agtcaccggt aaagatgttg ttcttacaga actcaaaaaa tataaaacaa
     2401 aagagatgac tttagttgag gtatatgatc tcggcaaaag attaaagtct gattatgtag
     2461 tttggggaag tatctctaaa attggcagca gtatcagcat cgatggaaaa ttagttgata
     2521 ttgccgccgg taaagcagac atcggcattt tcactcaatc gcaaagtctc gatgaagtga
     2581 ttccaaaaat taacgacttt tcccaacgaa ttgtgcaaca tattctcggg gtgacaccgc
     2641 catcagcgcc ggctgttgcc gcccccgctc cgacggttac tcgcccaaca gcacctggat
     2701 tatcccgtga atctcaaata attacaggca tgaaaacggg aaagagaggt actctgactt
     2761 ctgcaatcaa tactgaattt attaacgctc ctgatcctct taatcgcagg ggattctgga
     2821 tgagtcaaaa attacctact gaatttaaag gtatggatat aggagacgta aataacgacg
     2881 gtttaaacga agtagtggtt attgacagtc ataatgtata catataccaa aaaacagcca
     2941 atgaattgaa actattggag aaaatagctg gaaaatcctc tgataattat attgccgtgg
     3001 atgtggcaga tatcaacaga gacggtgtaa agcaaatatt cgtcacttct ctcaatcata
     3061 ccactcttga ttcttttgt
//
LOCUS       DASVIM010000174         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_411845, whole genome shotgun sequence.
ACCESSION   DASVIM010000174 DASVIM010000000
VERSION     DASVIM010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411845"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..680
                     /gene="raiA"
                     /locus_tag="VF305_03485"
     CDS             147..680
                     /gene="raiA"
                     /locus_tag="VF305_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX7416228.1"
                     /translation="MKISVTFRNGEGEKWQKVYAEERIQKLKKYLDTPAEAHIIVSME
                     KFRNFAEINLSSNGWNINAKEEAKDMHLAVDGCIEKIEKQLKKQREKIREHKPRSIRH
                     GKEKSADGEETEESTANKIAETRKVVLKPMSLDEAVMEIEGTNAHFIIYRDSSSENVS
                     LVYRRDDGNYALIETNS"
     gene            745..1212
                     /locus_tag="VF305_03490"
     CDS             745..1212
                     /locus_tag="VF305_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX7416229.1"
                     /translation="MQLDQIFKIEFIKGDLLAKTKTEALTELVNTVVQGGLKLDSSLA
                     IEVLQQRENLGSTGIGDGVAIPHGKISGLDELVVAFGRSKEGIAYDSLDGKPVHIFFL
                     LLAPENSAGQHLKVLAKISKMLKTVNFRKKLLEAKSERDLYKIIIDQDEVCIY"
     gene            1231..1386
                     /locus_tag="VF305_03495"
     CDS             1231..1386
                     /locus_tag="VF305_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416230.1"
                     /translation="MKNKLTLEDIFQQSSVKPGIKEIYSPAGLKKKIFAVKINNYYYH
                     LLSMNII"
     gene            1420..1881
                     /locus_tag="VF305_03500"
     CDS             1420..1881
                     /locus_tag="VF305_03500"
                     /inference="COORDINATES: protein motif:HMM:NF019118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416231.1"
                     /translation="MQEMINFHGVALEFNQMGILITGPSGIGKTTSALRVVQEGHYWI
                     ADDIVVVKRNQKGELVVRGHAKIKKLLHTKKTGIVPVKEILDSNRIKNQTNLAGIIEV
                     RNACISGCSSVTGEIEILGTKLPCLQIRIPSPGYFNKNLLKKAILQLQKDS"
     gene            1882..2748
                     /gene="rapZ"
                     /locus_tag="VF305_03505"
     CDS             1882..2748
                     /gene="rapZ"
                     /locus_tag="VF305_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX7416232.1"
                     /translation="MKNLRVVIITGLSGSGKSTALRALEDIGFFCVDNLPVVLLPKFL
                     AITTLSSPEIKQVALVMDLRERSFLDKYQRIFTRLKEKGYKIEILFIEASDDSLLHRF
                     SETRRIHPVSEKGPIMEGILKEREKLSSLKKMANKVIDTTSANVHQLKDTVQRHFLPS
                     SGHKKMVINVTSFGYRYGLPADADLVFDVRFLPNPYFVENLKNYDGHHVDVKDYVLQN
                     KESKEFLEKILDLMILLIPLYEKEGKVRLNVALGCTGGKHRSVVMANELGSYFSTMKY
                     IVNLNHRDINKS"
     gene            complement(2797..4122)
                     /locus_tag="VF305_03510"
     CDS             complement(2797..4122)
                     /locus_tag="VF305_03510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7416233.1"
                     /translation="MKKLSLYIKVSIAILIVAVLCWLYFGNYLEREKPIIKFNQEVKF
                     IGKQKIIEITFSDSKSGLSRLTAEIIQDNKGQILIDKIIPARGKEQEILPVNIDSVAL
                     KLHDGAAIINITAKDHSLFKNQTILSQEVIIDTIPPQITLFKAINNINQGGTCFIAYR
                     TSKPTPTTGVYVNDYFTPGYTLLFDNKPTVVTYFAIPINATKTKTKITVFARDDAGNQ
                     LNIALPCTIKGKKFRTDKMNLSENFLQQKMPEFQAMVPALKDKALLDVFIYVNSQMRN
                     ENFLTIQAICQKSTPKKLWEGTFLRMRKAAPMALFGDKRYYLSAGKVVAESLHVGVDL
                     ASTAQAAIEAANNGIVVFAGTLGIYGNAVIIDHGLGLFSLYGHLSSIETTVGKNVQEK
                     EIIGRSGMSGLAGGDHLHFSIIVGGQFVIPQEWWDPHWIKDNINKKMLF"
     gene            4419..>4645
                     /locus_tag="VF305_03515"
     CDS             4419..>4645
                     /locus_tag="VF305_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX7416234.1"
                     /translation="MLKGKKIVLGITGGIAAYKAAELTRALIKEGAQVKVIMTKCAAE
                     FITPLTLQTLSQNQVYTDMFLSADKYDMAHIA"
BASE COUNT         1510 a          795 c          950 g         1390 t
ORIGIN      
        1 taaaagagaa atgatgtaaa attgtgattg acttttagtt ttccgatcaa tatatgactg
       61 gcctctattg ccagtccggt ggtaagtttc aaaagtccag cttacttacg tgtcgaacag
      121 attaacctaa tcagggagga acaacgatga agatttcggt aactttcaga aacggtgaag
      181 gtgaaaaatg gcaaaaggtt tatgcggaag aaagaattca gaagctgaaa aaatatttgg
      241 atacgcctgc cgaagcacat attatcgttt ccatggagaa attcaggaat tttgcagaaa
      301 tcaatttgag ctctaatggt tggaatataa atgcaaaaga agaagccaaa gacatgcatc
      361 ttgcggtcga tggctgtata gaaaaaattg aaaagcaact taaaaaacaa agggaaaaaa
      421 tcagggagca taagcccaga agtattcgcc atggcaagga gaaatcagct gacggggaag
      481 aaacggaaga atcaacggca aacaaaattg cggaaacgcg taaagttgta ttgaagccaa
      541 tgtctcttga tgaagcggtg atggaaattg aaggaaccaa tgcccacttt attatttatc
      601 gagattcttc ttctgaaaac gtaagcttgg tttatcgtcg cgatgatgga aattatgcgc
      661 tgatcgagac aaatagctaa acggctggcg tctaaaacga gcttatgcat aaaattatga
      721 taaaagcatg aaaaggcgtg ttaaatgcaa ctagatcaaa tttttaaaat tgaatttatt
      781 aaaggcgatt tattggccaa aactaaaacc gaagctttaa cggagttagt taataccgta
      841 gttcaaggcg gccttaaact tgattcttct ttggctattg aagttttaca acaaagagaa
      901 aatcttggtt ctacgggaat aggtgacgga gtggcaattc ctcatggaaa aatatctggg
      961 cttgatgagc ttgttgtcgc ttttggacgt agtaaagaag gtattgctta tgattcatta
     1021 gatggcaagc ccgtgcatat ttttttcttg cttttggcgc cggaaaattc agccggtcag
     1081 catttaaaag ttttggccaa gatttccaaa atgttgaaaa cggttaattt ccgtaaaaag
     1141 cttttggaag caaaatccga acgcgatttg tataaaataa ttattgatca agatgaggtc
     1201 tgcatatatt aaactttaaa ttggacatcc atgaaaaata aattaactct ggaagatatt
     1261 tttcagcaga gttcagtaaa gccaggcatc aaagaaatct attctccggc aggattaaaa
     1321 aagaaaatat ttgcagtcaa aatcaataat tattattatc atctgctttc aatgaacatt
     1381 atttaaatga catgttaatg aggattaatc tgtgaaaaga tgcaagaaat gataaatttt
     1441 cacggcgttg ctcttgaatt caaccaaatg gggatattaa ttactggccc tagcggaatt
     1501 ggaaaaacaa cttcagcctt gcgtgtggtt caagagggac attactggat tgctgatgat
     1561 atcgttgttg tcaaaagaaa tcaaaaaggg gaattagttg tccggggcca tgcgaaaatt
     1621 aaaaaattat tgcatacgaa gaaaacagga attgttcccg taaaagaaat attggattct
     1681 aatagaatta aaaatcaaac aaaccttgcg ggaatcatag aagttagaaa tgcctgtata
     1741 tctggatgct cttcggttac tggagaaata gagattctgg ggaccaaatt gccttgtttg
     1801 cagataagaa ttccctctcc tggctatttt aataaaaacc tgctaaaaaa agccatcctg
     1861 cagttacaaa aggatagtta aatgaaaaac ctgcgagtcg taattattac cggtctttcc
     1921 ggttccggta aaagtacagc cttgagggca ttggaagata ttgggttttt ttgtgtggat
     1981 aacttgccgg ttgttttact accgaaattt cttgctataa ccacgctttc atcgccggaa
     2041 ataaaacaag tagccctggt catggatttg cgggaaagaa gctttctcga caagtaccag
     2101 cgaatattta cacgccttaa agaaaagggt tataaaattg agattctctt cattgaagcc
     2161 agcgacgatt ctcttttgca tcgctttagc gagaccagac gtattcatcc tgtctccgaa
     2221 aaaggaccga tcatggaagg catcctgaag gaaagagaaa aattatcgtc cttaaaaaaa
     2281 atggcgaaca aagttatcga tacaacttcc gctaatgttc accaattaaa agatacagtc
     2341 cagcggcatt ttctgccctc ttcgggacat aaaaaaatgg tcattaacgt aacttctttt
     2401 ggctatcgtt atggcttacc cgctgatgcc gatctcgttt ttgatgtaag gtttttacct
     2461 aatccttatt ttgttgaaaa tttaaaaaat tatgacggcc atcatgtcga tgtgaaagac
     2521 tacgttttac aaaataagga aagtaaagaa tttttggaga aaatattaga tttaatgatt
     2581 ttactcatcc ccctctatga aaaagaaggt aaggtgcgtt taaatgttgc cctgggctgt
     2641 acaggcggaa aacaccgttc cgtagttatg gccaatgaat taggttcgta tttttcgacg
     2701 atgaagtata tagtcaattt aaatcatcgc gacatcaaca aaagctaacc ggacgcttta
     2761 taaataaccc tatctactgt tgctgttgtt aatatatcag aagagcattt ttttattgat
     2821 attatcttta atccagtgcg gatcccacca ttcttgcgga ataacaaatt gtccaccgac
     2881 tatgatgctg aaatgtaaat gatctcctcc cgcaagcccg gacatgccgg aacgaccgat
     2941 tatttctttt tcctgtacgt ttttaccaac ggtagtttcg atagaagata aatgtccata
     3001 gaggctaaat agccctaaac catgatcaat aataacggcg tttccgtaaa tacctaaagt
     3061 tcccgcgaag acaacaatgc cgttgttagc tgcttcaata gccgcctgag cggttgaagc
     3121 caaatcgact ccgacgtgaa gggattcagc aacaacttta cccgcagata aataatatct
     3181 cttgtcacca aaaagtgcca tgggggcagc tttacgcatg cgcaaaaaag ttccttccca
     3241 aagttttttt ggtgtggact tttggcaaat agcctgaatg gtcagaaaat tttcattacg
     3301 catttgagaa ttgacataga taaaaacatc caaaagtgcc ttatctttta gcgctggaac
     3361 cattgcttga aactccggca ttttttgttg cagaaagttt tcgcttaagt tcattttatc
     3421 cgtgcgaaat tttttccctt taattgtaca agggagtgca atgtttaatt gatttcccgc
     3481 gtcatcgcgg gcaaagacgg ttattttcgt ttttgttttg gttgcattaa tcggtatagc
     3541 aaaataggtt acaacagttg gcttgttatc aaacaataag gtgtagccag gtgtgaaata
     3601 atcattaaca taaacgcctg tcgtaggagt gggtttggat gtgcggtaag cgatgaaaca
     3661 tgtgccacct tgatttatat tgttgattgc tttgaacaga gtaatctgcg gcggtattgt
     3721 atcaattatt acttcttgag ataggatggt ttgattctta aaaagagagt gatctttagc
     3781 tgtgatattg atgatcgccg caccatcgtg gagtttaagc gcgactgaat ctatatttac
     3841 gggtagaatt tcctgttctt tacctctggc aggaattatt ttatcaatta aaatttgacc
     3901 tttattatcc tgaattattt ctgcggttaa acgggaaaga ccgcttttgc tgtcggaaaa
     3961 agtgatctca attatttttt gtttaccaat gaatttaact tcctggttaa actttattat
     4021 aggtttttct ctttccaaat aattgccaaa ataaagccag caaagcacgg cgactattaa
     4081 aattgcgatg gaaactttga tatataaact taattttttc ataataatct cctcaagcta
     4141 tgagataaaa cagctgcgcg gcttataatg gcttttagca tcttttgcaa ggcaattttc
     4201 ctttttcacc tgcataaaaa aaatttgaag aatcctgcgc attaagacgc ggtaagctga
     4261 tacagctttt ttcttgtaaa agggtaaact atttaatatt caacgtaaaa ttataagaaa
     4321 aagctttacg tgatagagct aattttaagg tagttatacc ttgaacttgt gataaaacgc
     4381 tgttatatta ttttaaaatc aaacagagag acaagattat gcttaaggga aagaaaatag
     4441 ttttgggaat aacgggtggg attgccgcct acaaagcggc ggaattaact cgcgccctca
     4501 tcaaggaagg cgcgcaggta aaagtcatca tgacgaagtg tgctgccgaa tttattacac
     4561 ctttaactct gcaaactctt tcgcaaaatc aagtttatac ggatatgttt ctttcggcgg
     4621 ataaatacga tatggcccac attgc
//
LOCUS       DASVIM010000175         3347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_837431, whole genome shotgun sequence.
ACCESSION   DASVIM010000175 DASVIM010000000
VERSION     DASVIM010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837431"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VF305_03520"
     CDS             <1..629
                     /locus_tag="VF305_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7416235.1"
                     /translation="MFEGMGGQLPGPTAMVIAASEFAQNYWWVILGVLVAIAIAFNRF
                     YKTEKGRWIVDSLSLKAPIFGLLLKKVAVAKFSRTLSTMMSSGVPILEGLNIVSKTSG
                     NRVVENALLKTRQSISEGRSIAEPLAETGIFPPMVVQMISVGEATGALDSMLNKIADF
                     YDDEVDAAVTAMTTLLEPVMMVFLGGIVGAMIIAMYLPIFKMAALAGG"
     gene            complement(666..742)
                     /locus_tag="VF305_03525"
     tRNA            complement(666..742)
                     /locus_tag="VF305_03525"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(706..708),aa:Pro,seq:cgg)
     gene            1138..1554
                     /locus_tag="VF305_03530"
     CDS             1138..1554
                     /locus_tag="VF305_03530"
                     /inference="COORDINATES: protein motif:HMM:NF018391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX7416236.1"
                     /translation="MIIYDLKCENGHKFEGWFNDQQAWISQNSQKIVSCPVCNSSNVE
                     IVPSSITIMGKDLRAAGKLQEKELSPAKALQMFHHYIDKNFEDVGNKFAEVALKIHYG
                     EEEKRNIKGTTTIQEENNLKEEGVQFIKIPVLKMDS"
     gene            1603..2424
                     /gene="amrB"
                     /locus_tag="VF305_03535"
     CDS             1603..2424
                     /gene="amrB"
                     /locus_tag="VF305_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX7416237.1"
                     /translation="MDTLRKSAIAGSWYPGEPSILRRDIENYFNSVPDLELEGEIVGL
                     IAPHAGYVYSGQVAAYAYKLICGKKYDAVIVIGPSHRIAFSGVSIYSKGGYETPLGIV
                     PIDESLAKEVKDLSKIIEDIPAAHLQEHSVEIQLPFLQVSLGDFSFVPLVMGDQGANT
                     CQELAETIYRVTRGKKLLIVGSSDLSHFHNYNAATKLDAVVLQHLKDADATGLLKSLA
                     GEATEACGGGPMVVAMLVANKMGANKARLLKYANSGDVTGDKSSVVGYAAAVYYI"
     gene            2610..3158
                     /gene="amrA"
                     /locus_tag="VF305_03540"
     CDS             2610..3158
                     /gene="amrA"
                     /locus_tag="VF305_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX7416238.1"
                     /translation="MELTTKEKSTLLDIVKKTIAAKVNNKELPKLTVDSETLKEKRGA
                     FVTLKKRGHLRGCIGYIKAVKPLWETVQEMAVAAAFHDPRFPSLMPDEVRDLTFEVSV
                     LSPLKRIKDINEIEVGKHGLYIVRGYNSGLLLPQVAAEYKWDRESFLNETCYKAGLPA
                     QAWRDEETEIYTFSADYFGDTD"
BASE COUNT          977 a          557 c          799 g         1014 t
ORIGIN      
        1 aaatgttcga gggtatgggc ggacaacttc ccgggcctac ggcgatggta attgcggcta
       61 gtgaatttgc acaaaattat tggtgggtta tacttggtgt attagttgct attgctattg
      121 ctttcaacag gttttacaaa acggaaaaag ggcgctggat agtggattct ctatcgctta
      181 aggcgccgat cttcggccta cttttgaaaa aggtagctgt ggccaaattt tcgcgtacgc
      241 tttcgacaat gatgagttcc ggcgtgccca ttttggaagg attgaatatt gtcagtaaaa
      301 cttccggtaa tagagttgtc gaaaatgctt tgttaaaaac acgccagagt attagcgagg
      361 gacggtctat agcggagccg ctggccgaaa cgggtatttt tccgcccatg gttgtgcaaa
      421 tgatttctgt gggagaggcg acgggcgccc ttgattctat gctcaataaa attgctgact
      481 tttatgacga tgaagttgat gccgcggtaa ctgccatgac aacgcttttg gagcctgtaa
      541 tgatggtttt cttaggggga atcgttggcg ctatgattat cgcgatgtat ctgccgatct
      601 ttaaaatggc agctcttgca ggtggttaag actgtgcaag attcgcagct tttaagatag
      661 aactatggtc gggatggcgc gatttgaacg cgcgacccct gcgtcccgaa cgcagtgccc
      721 taccaaactg ggccacatcc cgacacggta cggacgatat aaaatttatt tctaaatgtc
      781 cacatttttt tattcaattt agatattgcc gttaataaat ctcagcattg gtattgcgct
      841 gtccgtcagt tagtgccgcc gttattcaat aatttgaaag cttttaaaaa caacttcgcc
      901 actgctcaaa atatttttcg tgtgtgatag gggaaattag gatcgtgacc aatatcaagc
      961 agatattgcg atcactggat agacggaaat tactagagta gtagcttgta aactattttt
     1021 cagttgttat gaaaatctct aaatttatat atatggcata atatacacgt gtaattgtct
     1081 tttatttaat ggcgtgctag aataatgtta atttattgtg gagagggtat taatcaagtg
     1141 attatttatg atttaaaatg tgaaaacgga cataaattcg aaggttggtt taatgaccaa
     1201 caggcatgga tatcacaaaa ttctcagaag atagtttctt gccctgtatg taatagttcg
     1261 aatgtggaaa tagttccttc ttcaattacg attatgggaa aagatttgcg ggcggcaggt
     1321 aagcttcagg aaaaggaact ttctccagcc aaagcgttac aaatgtttca tcattatatt
     1381 gataaaaact tcgaagatgt gggcaataaa tttgccgaag tcgccttaaa gatacattac
     1441 ggtgaagaag aaaaacgcaa tatcaaaggg acaactacga tacaagagga aaacaactta
     1501 aaggaagaag gcgttcagtt cattaaaatt cctgtgttaa aaatggatag ctaatccatt
     1561 ttttatctcg gatacttttt aggtaatata gggggtatcg atatggatac actgagaaaa
     1621 tcagcgatag ccggttcttg gtatcccgga gaaccttcca ttttgagaag agatattgag
     1681 aattatttca attcggtacc cgatttagaa ttggaagggg agattgtagg cttaattgcc
     1741 ccgcatgccg gttatgtgta ttcaggacag gtggcggcat acgcttataa gcttatttgc
     1801 gggaaaaaat atgatgcggt gattgtgatt ggcccgagtc atcgtatcgc cttttccgga
     1861 gtgtcgattt acagtaaggg tggttatgaa acgcccttgg gcattgttcc tattgatgaa
     1921 tcgttggcca aagaagttaa ggatttaagt aaaataattg aggatatacc ggcggcgcat
     1981 ctgcaggaac attctgtaga aattcaactt ccttttttgc aagtgtctct gggtgatttt
     2041 tcatttgtac ctttggtgat gggagatcag ggtgcaaata cctgccagga acttgctgag
     2101 acaatttatc gggtgacacg cggtaagaaa ctattaattg tgggtagttc cgatttatct
     2161 catttccata attacaacgc ggcgactaag ttagatgctg ttgttttaca gcatctaaaa
     2221 gatgccgatg cgaccgggct tttgaaaagt ttagccggtg aagccactga ggcatgcggt
     2281 ggagggccaa tggtagtggc gatgctagta gctaataaga tgggtgcaaa taaagcccgg
     2341 cttttaaaat atgcaaattc cggagatgta accggagata agagttcggt tgtcggatac
     2401 gcggcagcag tttattacat ataatgacgg ttttataaaa agtccatctg ctaagttgcg
     2461 ctgtatcatt acggattacg tctgaacttt gccttattct atgggtttta cgctcattgt
     2521 tactggagct ttttacaaag ttgctcagtt tgtgggaatt taggattttt ttgtctccat
     2581 aaaatgatta gagttaataa gaggggaaaa tggaattaac aacaaaggaa aaatcaactc
     2641 ttctggatat tgtaaaaaaa actattgcgg ctaaagtcaa caacaaggaa ttaccgaaat
     2701 taactgtgga ttcggaaacg ctcaaagaga agagaggcgc atttgtgacc ctgaaaaaac
     2761 gtggccattt gcgtggttgc atcggctaca taaaggcagt taaaccgctt tgggaaactg
     2821 tccaggaaat ggctgtggcg gcagcttttc atgatccacg gtttccatcg ttgatgcccg
     2881 atgaggttcg agatttaact tttgaagtat ccgtattgag ccctttaaag cgtattaaag
     2941 atattaatga aattgaagtg ggaaagcacg gtttatacat tgtccgcggt tacaattccg
     3001 gtttattatt gccacaagtc gcagccgaat ataaatggga cagagaatct tttttaaatg
     3061 agacttgtta caaggccggt ctgccagctc aggcttggag ggatgaagaa acggaaattt
     3121 ataccttttc ggcagattat tttggcgata cagattagct gattgttaat cattttacct
     3181 attacgctat aggagcgtcg gttatattta tggcaatctt gtatttattt aacggtcaga
     3241 tcattgattg gtgtatgaaa gatagattga tgcaggaaat ttattttaaa agatgcttaa
     3301 tcaaacttta gggcgaaaaa aaattaaatc cgaaatttat atatttc
//
LOCUS       DASVIM010000176         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_412307, whole genome shotgun sequence.
ACCESSION   DASVIM010000176 DASVIM010000000
VERSION     DASVIM010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412307"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VF305_03545"
     CDS             <1..657
                     /locus_tag="VF305_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7416239.1"
                     /translation="LVGVSGGADSFALLDLLDSPMIFTPRFSLLAVNIDMGCDISYSG
                     YNALEKYLQGNNYRYVMEKTDIGLLAHSDYNRKNPCFLCSRLRRKRIFEIAAAEGCNK
                     IAFAHHRDDIIETLLINMFYGREISTMMPNQKIFGGKLHIIRPLAYLQEELIKKYQSE
                     RFFPTAENNCPTSKKSRRMYVKNLLNELARENKDIRDNIFKAMSHVKPDYLLPLSKKK
                     "
     gene            689..1165
                     /gene="smpB"
                     /locus_tag="VF305_03550"
     CDS             689..1165
                     /gene="smpB"
                     /locus_tag="VF305_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX7416240.1"
                     /translation="MVKENTAQKLIASNKTARRNYDITDTYEAGMVLTGTEVKALRAG
                     KANLKDSYALVNNEEVFLRELHISHYDHGNRYNHDPLRARKLLLHKREIRKLYGKSRE
                     KGLALIPLKLYFKNGKVKVEIGIGKGKKLYDRREDIKKREERRDIARDFKSKLIKV"
     gene            1234..2148
                     /locus_tag="VF305_03555"
     CDS             1234..2148
                     /locus_tag="VF305_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX7416241.1"
                     /translation="MKCRKIRKVIKSKPTLEGAGVHLKRAFGFSEVPLLDPFLLLDDF
                     RSGNPGHYLKGFPWHPHRGIETITYMLGGNVEHGDSMGNKGVIGPGDVQWMTAGSGII
                     HQEMPKGDKTGYMGGFQLWANLPAAHKMMPPRYRDVKVSQIPEISTKDGVKIKIICGE
                     IEGTKGPVHDIVTDPFYCDVTIPAKTEFTYPTKPGHTVFAYIIEGKASFCKESNPFVY
                     EVEGVNYFDIHRDAYLTNGNLVLFDDGEKVVVSTKDEPVRFLLISGKPIGEPVAWYGP
                     IVMNTQQELQIAFEEYDNGTFIKHKRLS"
BASE COUNT          725 a          440 c          523 g          562 t
ORIGIN      
        1 ctggtcggcg tatccggagg ggcagacagc tttgcgctct tggatttact ggattcaccg
       61 atgatattca ctccgaggtt ttcccttctg gccgtcaata ttgatatggg ttgcgacatt
      121 tcttacagcg gctacaatgc actggaaaaa tatttacagg gaaataatta ccgctatgtg
      181 atggagaaaa cggatattgg tcttttggcg catagtgatt ataataggaa aaatccttgt
      241 tttctatgct cgcggctacg ccggaaaaga atttttgaaa tcgccgccgc tgaaggctgc
      301 aacaaaattg ccttcgctca tcaccgcgat gacatcatcg aaacattgct gattaacatg
      361 ttctatggcc gtgaaatcag cacaatgatg cctaatcaga agattttcgg cggtaaactg
      421 catatcatcc ggcccctggc ctatctgcag gaggaactta ttaaaaaata tcaaagtgag
      481 cggtttttcc cgaccgcgga aaataattgc ccgaccagca agaaatcaag aagaatgtat
      541 gtgaaaaacc ttttaaatga actggctcgc gaaaataaag atattcggga taatattttt
      601 aaagccatga gtcatgtaaa accggattat ttactgcctt tatcaaaaaa gaaatagcaa
      661 tattcgtatt taaatgtaaa aattaaccat ggtcaaagaa aatacagcac aaaaattgat
      721 tgcctccaac aagactgctc gccgcaacta cgacataaca gatacttacg aggccggtat
      781 ggtcctgacc ggcacagagg tcaaggcgct gcgcgccggc aaggcgaact tgaaagacag
      841 ttacgctctg gtcaataacg aagaagtatt cctgcgcgaa ttgcatatca gccattatga
      901 tcacggtaac cgctataatc atgatccgct gcgggcgcgc aaactcctgc tgcataagag
      961 ggaaatcagg aaactctacg gcaaatcgcg ggagaaaggt ttggcgttga ttccactaaa
     1021 gctttatttt aaaaacggca aagttaaggt ggaaattgga ataggcaaag ggaagaaatt
     1081 gtacgaccgc cgtgaggata tcaagaaacg cgaagaacgc cgtgacattg cccgtgactt
     1141 caaatcaaag ctgataaaag tttaacaaga aaacaaaatt aaatggattg aggaatatta
     1201 aagactaaaa attgatataa aaggggcatg atgatgaaat gcagaaagat acggaaagtc
     1261 atcaaaagca aaccaactct ggaaggagcc ggtgttcatt taaagcgggc tttcggtttt
     1321 agtgaagttc cgctgttgga tccgtttttg ttactggacg attttcgatc cggcaatccc
     1381 ggccattacc ttaaaggctt cccctggcat ccgcaccggg ggattgaaac aattacttat
     1441 atgctgggag gaaatgtcga acatggagac agcatgggca ataaaggtgt gatcggtccc
     1501 ggagatgtgc aatggatgac agccgggagc ggcatcatcc accaggaaat gcccaaaggc
     1561 gataaaacag gctatatggg aggatttcag ctttgggcta atcttccggc agcgcataag
     1621 atgatgccgc cgcgttatcg tgacgtaaaa gtgagccaaa tcccggaaat ttccacaaag
     1681 gacggtgtaa aaatcaaaat catttgcggc gaaattgagg gaacaaaagg acctgtccat
     1741 gatatcgtta ccgatccttt ctattgtgat gttacgattc cagctaaaac ggaatttact
     1801 tatccgacca aaccagggca cacagttttt gcctatatta tcgaaggcaa ggctagtttc
     1861 tgtaaggaaa gtaatccgtt tgtctatgaa gtcgaagggg taaactattt cgacatccat
     1921 agagacgcgt atttaacaaa cggaaatctt gttttattcg atgatggaga aaaggtagtt
     1981 gtttccacaa aagacgaacc tgttcgtttt cttttaatat ccggtaaacc catcggtgaa
     2041 ccggttgcct ggtatggccc tattgtaatg aacacacagc aagaattgca gattgccttt
     2101 gaagaatacg ataacggcac gtttataaaa cataaaagac taagttaaag taggcagaaa
     2161 atttcggatg cggctagcaa agttattgaa attacggtgt taatatttat agaaaagata
     2221 ctgaaaatcc tccttttggt aaggggaaaa
//
LOCUS       DASVIM010000177         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_751764, whole genome shotgun sequence.
ACCESSION   DASVIM010000177 DASVIM010000000
VERSION     DASVIM010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751764"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /gene="murC"
                     /locus_tag="VF305_03560"
     CDS             complement(<1..1051)
                     /gene="murC"
                     /locus_tag="VF305_03560"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX7416242.1"
                     /translation="MRVDRETSLMQRKVKCIHFVGIGGIGMSGIAEVLINLGYKISGS
                     DAQQSETTVRLQKLGAAVALGHAAKNIGHADVVVTSTAVKADNPEVIEAHRKNTPVIP
                     RAEMLAELLKMKFSVAVSGSHGKTTTTSMISTILALGGLDPTMVIGGKLASIGSNARL
                     GDGEIIVAEADESDGSFLKLSPTIAVITNIDREHLDFYPGITEIKEAFCKFANIVPFY
                     GCTVLCSDNLHVREIAPLIKRKMITYGIEQSADYSAREIKFLADKTIYNLSYKGEPQG
                     EIGLVVPGMFNVYNSLAAIAVCRELDLSLPTIKKGLLGFSGVQRRLEIKGKIADIIVV
                     DDYGHHPTEIKETLTA"
     gene            complement(1092..2177)
                     /gene="murG"
                     /locus_tag="VF305_03565"
     CDS             complement(1092..2177)
                     /gene="murG"
                     /locus_tag="VF305_03565"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX7416243.1"
                     /translation="MHIVIAGGGTGGHLFPGIAIAEEFLRRDDKAEVIFIGTKKGIES
                     RLLGPLGYELREIDIEGVKGRGFKALVRGVYQIPLSMWQARRILKKFGPHIVIGVGGY
                     VSGPAVLTAHFMNIPTVIAEQNAVPGITNRILGKFTNKIFVTYTQTQKYFPQDKVILS
                     GNPVRAAFAAGRSKAKEKKDFWQLLIFGGSQGAEAINKAVINMLPQLQNMKNKVRVVH
                     QTGARQFEKVKKTYKQFGIQVKVLPFIVDMAGAYAAADLIICRAGATSLAEITAAGKA
                     AILIPYPWAANDHQTKNAQAMAKAGAAVVIKENELTSSKLFGVVKNLLNNEKKLEDMK
                     EKSAQLGNINAAVNIVDACMELLSKKN"
     gene            complement(2252..3361)
                     /gene="ftsW"
                     /locus_tag="VF305_03570"
     CDS             complement(2252..3361)
                     /gene="ftsW"
                     /locus_tag="VF305_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX7416244.1"
                     /translation="MDAAVKNENKLPDIILLLVTLILVTVGTAMIYSSSSIIALERFK
                     DGQYFLKKQILFVFLGLISMVLMTKIPYTQLRKWAYPGMLLSLLLLAILLIPNVGIKR
                     GGATRWLNMGVFSFQVTEMVKISMVVFLAHLLARKAHQLKKFSKGILLPMVITSVVIL
                     LVLLEPDFGTAAIIAAILILMLCIAGSRVTHILLLMAAFIPVGVLLVLYKGYRVARLT
                     AFLDPWKDASNTGFQIIQSLLSFGSGGTFGVGIGDGMQKLFYLPEPHTDFILSIIAEE
                     SGFIGVTIVVLMFAIFAFRGFMIALKAPDLFGTLLAVGLTMVIALESFINIAGVMGLI
                     PLKGLVLPFLSYGGTAFIMSMTAVGILLNISTQTA"
BASE COUNT          978 a          859 c          610 g          974 t
ORIGIN      
        1 cagcagtcag tgtctctttt atttccgtcg gatggtgtcc ataatcatcg acaacaatta
       61 tatccgctat ctttcctttt atttccaaac gtctctgcac accggaaaag ccaagcaatc
      121 cttttttgat ggtcggcaaa ctcaaatcca attctcgaca taccgcaatt gccgccagag
      181 aattatatac attgaacatc cccggcacga ccaagccgat ttctccctgg ggctctcctt
      241 tataactaag attgtaaata gttttgtctg ccagaaattt tatctcccgg gcactgtagt
      301 ccgcagactg ctcaatgcca taagttatca tcttgcgttt gatcaacgga gcaatttccc
      361 gcacatgcag attgtcgctg caaaggaccg tgcaaccata aaagggaaca atattggcaa
      421 acttgcaaaa cgcctctttg atttccgtaa tgcccggata aaaatccaaa tgttcgcggt
      481 cgatattcgt aattaccgca atggtgggcg ataattttaa aaatgagccg tcgctttcat
      541 ccgcttccgc cacaatgatt tcaccatcac ctaatcgggc gttgctgccg atgcttgcca
      601 gttttccccc gatgaccatt gtcggatcta aaccgcccag tgcaagaatc gtcgaaatca
      661 tcgatgtagt cgtagttttg ccgtggctgc cggagacggc aacagaaaat ttcatcttga
      721 gaagttccgc cagcatttcc gctctcggaa ttaccggagt atttttccgg tgcgcttcta
      781 taacttctgg attgtccgct tttactgctg tagaagtaac aacgacatcg gcatgaccga
      841 tatttttcgc cgcatgccct aaagctaccg cagccccaag cttctgcaag cgcaccgttg
      901 tttccgactg ctgagcgtcg gaaccgctga tcttgtagcc gagattaatt agaacttcgg
      961 caatcccgct catgccgatg cccccaatcc cgacaaaatg aatgcatttt actttgcgtt
     1021 gcattaagct ggtttctcta tctactctca tttctcaaca ttctctttcc atttttacta
     1081 acaataaatc cttaattttt cttgcttagt aattccatgc aggcatcaac tatatttacc
     1141 gctgcgttta tatttccaag ttgggccgat ttttctttca tatcctccaa ttttttttca
     1201 ttattaagca aatttttaac cacaccgaat aacttgctgc tggtaagctc gttctcttta
     1261 attacgacgg ccgcaccggc cttggccata gcttgggcgt tttttgtttg atgatcatta
     1321 gccgcccaag gataaggaat taaaattgca gctttacccg cagcggtaat ttcggcaagc
     1381 gaggtggcac cagcgcgaca aatgattaaa tctgcagcgg cataagctcc ggccatatca
     1441 acaatgaaag gcaaaacctt cacctgaatg ccgaattgtt tatacgtctt cttcactttt
     1501 tcaaattgcc ttgctcccgt ctgatgcacg acacgaacct tatttttcat attctgcagt
     1561 tgcggcaaca tattgatgac ggccttattg attgcctccg ctccttgaga tccaccaaaa
     1621 ataagcagtt gccaaaaatc tttcttttct tttgctttgc tgcgtcccgc ggcaaatgca
     1681 gcccgcactg gattgccgct taaaatcact ttgtcctgcg gaaaatattt ttgggtttgt
     1741 gtatatgtca cgaatatctt atttgtaaac ttacccaaaa ttctgttggt aataccaggc
     1801 acagcatttt gttccgctat tactgtgggt atattcataa aatgggctgt aagtactgcc
     1861 ggacctgaaa cataaccgcc tacaccaata acaatatgcg gtccgaattt tttcaaaata
     1921 cgcctcgctt gccacatgct cagcggaatc tgataaacac ctcttaccaa tgccttgaac
     1981 ccccgtcctt taactccttc aatgtcaatt tctctgagct cataaccaag tgggccaagc
     2041 agtctgcttt caattccttt ttttgttcca ataaaaataa cttccgcttt atcatctctt
     2101 cgcaaaaatt cctcggcaat ggcaatgccc ggaaacaagt gtccgcctgt tcctcctccc
     2161 gcaattacga tgtgcatctt ttgctccaga caaaattaaa aaagtttaat ttctctttca
     2221 gcaaatacct atacgttcct cgatctttca tttatgcagt ctgagtggaa atattcaata
     2281 aaatacccac agccgtcata ctcatgataa atgccgttcc gccataactc aaaaaaggca
     2341 gcaccaaacc tttcagtggt ataagcccca ttactccggc aatattgatg aatgattcca
     2401 aagcaattac catggtcaat ccaacggcca gcaatgtccc aaaaaggtcg ggcgccttta
     2461 gcgcaatcat aaagccacgg aaggcaaaaa tagcaaacat gagaaccaca atagtcactc
     2521 cgataaagcc gctttcttcg gcgatgatag aaagaataaa atcggtatgt ggctcaggca
     2581 aataaaataa tttttgcatg ccgtcaccga ttcccactcc aaaagtcccc ccggaaccaa
     2641 aagacagtag cgattgaata atttgaaaac cggtattgct ggcatctttc catggatcca
     2701 aaaatgccgt caaacgtgca accctatatc ccttgtacag aaccaacaat accccgacag
     2761 gaatgaaagc ggccattaaa agtaaaatat gcgttactcg cgaccctgct atgcacagca
     2821 ttagaatcaa aattgcagca ataattgcag ctgtgccaaa atccggctct aataaaacca
     2881 gcaaaatgac gactgaggta atcaccatcg gaaggagaat ccctttgctg aattttttta
     2941 gttgatgtgc ctttcgggcc aataagtgtg caaggaaaac caccattgaa attttaacca
     3001 tttctgttac ctgaaaagaa aacacaccca tattcagcca gcgtgtggct ccaccgcgtt
     3061 taattcccac attcggtatc agcagtatag ccaatagtaa tagagataat aacatccccg
     3121 gataagccca ttttcttaat tgtgtataag gaatcttagt catgagcacc atagaaatta
     3181 agcctaaaaa gacaaaaagg atttgctttt ttaaaaaata ttggccgtcc ttaaatcgct
     3241 ccaaggcaat aatagagctg gacgaataaa tcatcgccgt gcctacagtc accaaaatca
     3301 aagtaactaa aagcagaata atgtccggca atttattttc atttttgact gcggcatcca
     3361 tctaaagatt cctcactatc tctttaaaaa aatcaccccg ttctttataa ttggcaaatt
     3421 c
//
LOCUS       DASVIM010000178         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_751875, whole genome shotgun sequence.
ACCESSION   DASVIM010000178 DASVIM010000000
VERSION     DASVIM010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751875"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="VF305_03575"
     CDS             complement(<1..1406)
                     /locus_tag="VF305_03575"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX7416245.1"
                     /translation="MKLHPQLMNPKFYLMIFSDGIIFVIALVAAYLFRFEFSFNHVNI
                     QQIQNLIIWIVPLKLIIFLSSGLYRGMWRYTSIRDFWLLARASLLSTLLIMAIILYLN
                     RFVGYSRAVFIVDGFFTFLLTGGVRMIIRSYYAVYGSPKIIPSPLHKINLKKVLIIGA
                     GAAGEKILREIIENYQLQYRVVGFIDDDPEKQGRSIHGIRVIGNVEKIEQILKKEDVQ
                     EILIAMPSASGDQIRHVVEACRDCNVSYKILPGMGELIDGRVNIKVLRDINYEDLLGR
                     PPVHLNIKGIRNYLDGKTVLITGCGGSIGSELCRQVIKFQPRSVILLDVSEINLFHIQ
                     MEMQNERSYSKCEAILGHVQDKNLMNAIFKKYKPQVVFHAAAYKHVPMQEKNPWEAVF
                     NNIIGSRVTMDMSIKHHVERFVLVSTDKAVRPTNVMGASKRVTELIMQSAQGNDTRLM
                     AVRFGNVVGSSGSVIPIFR"
     gene            complement(1408..1968)
                     /locus_tag="VF305_03580"
     CDS             complement(1408..1968)
                     /locus_tag="VF305_03580"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019112620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7416246.1"
                     /translation="MKRVFDFIFALLLLVILFIPVLIVAIAIKLSSPGPVIYWSDRVG
                     KNNKIFSMPKFRTMCIDTPAVATHLLQNVEHYLAPLGSFLRRTSLDELPQLWSILRGN
                     MSFVGPRPALFNQDDLIKLRTEKEISKLQPGITGWAQINGRDDIPIPIKVLYDEYYMK
                     NQSFFFDLKILWNTFFKVIKKEGVVH"
     gene            complement(1965..>2307)
                     /locus_tag="VF305_03585"
     CDS             complement(1965..>2307)
                     /locus_tag="VF305_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416247.1"
                     /translation="LDNLIDVIMTCLTHPKANGKTYLVSDGEDRSTPELIRLMAVALG
                     KPGRLFYLPLFILRLGGWITGNSQTIARLTGSLMVDSSKIRRELGWKPFFSVEEGITE
                     TARFYKKECKT"
BASE COUNT          721 a          520 c          424 g          642 t
ORIGIN      
        1 cggaaaatcg gaattaccga accggaagaa ccgacaacat taccaaacct aacggccatt
       61 aagcgcgtat cattgccttg cgcggattgc atgatcaatt cggtaacccg cttgcttgct
      121 cccatgacat ttgtgggacg cacagcctta tccgtagata ccagaacaaa ccgttcgaca
      181 tgatgtttaa ttgacatatc catggtaaca cggctgccga tgatgttatt gaaaaccgcc
      241 tcccagggat tcttttcctg cataggcaca tgtttataag cggcagcgtg aaaaacaacc
      301 tgaggtttat attttttaaa aatagcattc attagatttt tatcttgtac atgtcccagt
      361 attgcctcac acttgctgta tgatctttca ttttgcattt ccatttgaat atggaacaaa
      421 tttatttcgc tgacatcaag caagataaca gaacgaggct gaaattttat tacctgccgg
      481 cataattcgg aaccgatcga tccgccacag ccggtaatta aaaccgtttt gccatcaagg
      541 taattgcgaa tacctttaat attcaagtgc accggcggcc gccccaaaag gtcttcgtaa
      601 ttaatatccc ggagcacctt tatattaacc ctgccgtcaa tgagctcgcc catccccggc
      661 aatattttat aggaaacatt acaatcccgg caagcttcga caacatgtct gatttgatcg
      721 ccactggcgg aaggcatagc aatgagtatt tcctgcacat cttccttttt gagaatctgc
      781 tctatttttt caacattgcc gataacacga atgccatgaa ttgatcttcc ttgcttttcc
      841 ggatcgtcat caatgaatcc aacaactcgg tattgcaatt gatagttttc aataatttcc
      901 cttaatattt tttcaccagc ggcgcctgct ccaataatca gcactttctt taaattaatt
      961 ttatgaagcg gacttgggat gattttagga ctgccataaa cggcatagta ggaacgaata
     1021 atcatgcgga ctccaccggt aagcaaaaac gtaaagaaac cgtcaacgat aaaaacagcg
     1081 cgggaatagc cgacaaacct gtttaggtaa aggataatgg ccataatcaa cagtgtggat
     1141 aagaggctcg cacgcgctaa gagccaaaaa tcccttatgc tggtataacg ccacataccg
     1201 cgatagagcc cggaggaaag aaatataatt aacttcaggg gaacaatcca gatgatcagg
     1261 ttttgaattt gctgaatatt tacgtgatta aaagaaaact caaagcgaaa caaataggca
     1321 gccacaaggg caattacaaa aatgattcca tcgctgaata tcatcaaata aaacttggga
     1381 ttcattagtt ggggatgtaa cttcatatta atgaacaacg ccttcctttt taatcacctt
     1441 gaaaaaagta ttccataaaa ttttcaggtc aaagaagaaa gattgatttt tcatataata
     1501 ctcatcatac aacactttaa tgggaattgg aatatcatct ctgccgttga tttgtgccca
     1561 tccggtaatg cccggctgca atttggaaat ttctttttcc gttcttaatt ttatcaaatc
     1621 atcttgattg aaaagcgccg gccgcggccc cacaaagctc atgtttcccc ggagtatgct
     1681 ccacaactgg ggtaactcat caagactggt tctacgcaag aatgacccaa gcggagctaa
     1741 ataatgctca acattttgca aaagatgcgt tgctactgcc ggagtatcta tgcacattgt
     1801 acgaaattta ggcatggaaa aaattttatt gtttttaccc actcgatccg accagtaaat
     1861 aaccggtccc ggcgaagata atttgatggc gatagcaaca atcaagacag gaataaacag
     1921 aatcaccagc aaaagtaacg caaaaataaa atcaaaaact cttttcatgt cttgcattct
     1981 ttcttataaa aacgagcagt ttccgttatt ccttcttcca ccgaaaaaaa cggtttccat
     2041 ccaagttcac gtcttatttt tgatgaatca accattagag aacctgtcag cctggctatt
     2101 gtctgtgaat tcccggttat ccaaccacca aggcgcaata taaacaaagg cagataaaat
     2161 aatcttcccg gtttaccaag cgctacggcc atgagcctga tcaattccgg cgtagatcta
     2221 tcttcaccat cactcacaag atatgttttc ccgtttgctt ttggatgtgt taggcacgtc
     2281 ataataacat caataaggtt atccaaa
//
LOCUS       DASVIM010000179         4218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1096283, whole genome shotgun sequence.
ACCESSION   DASVIM010000179 DASVIM010000000
VERSION     DASVIM010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096283"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1132)
                     /locus_tag="VF305_03590"
     CDS             complement(143..1132)
                     /locus_tag="VF305_03590"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX7416248.1"
                     /translation="MNMRILITGHLPENVISPLKEKYQVEMNQEDRPLDRQAIISGVK
                     DKHGLLPMLSDSINGEVLNCAPHLKMIANFGVGYNNIDVRAATARGIMVSNTPGVLTD
                     ATAELAFALILAVSRRIVEGDRMVREGRFKCWAPMLFLGREVTGKTLGIIGMGKIGKA
                     VAARARAFDMRILYHNRKRIDVEGEKALMAEYVDLKKLLIESDFVSLHVPLTEETKHL
                     IGRSELALMKPTAFLINTSRGPVIDEKALVEVLRARKIGGAGLDVYENEPSLTPGLTE
                     LDNVILLPHVGSGTLETRIKIGTLAVENLIAGLEGRKPPNLVNPEVLKMRKDI"
     gene            1366..2307
                     /gene="prmA"
                     /locus_tag="VF305_03595"
     CDS             1366..2307
                     /gene="prmA"
                     /locus_tag="VF305_03595"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX7416249.1"
                     /translation="MAEKEPEKWLKIEISAPAELMDALGNFLTETGAQGVFQESSAPQ
                     HTIEDFPEPVIEETLKAYFPQDARCEKRIAAVQKYINSLREIFPDFGKLSFTTEIIND
                     LDWGEQWKKYFKPIRVSNNIIVKPTWERYSPSSRDIVIEIDPGMAFGTGQHASTRMCI
                     EAIEDIMKDRSIKSWKVLDAGCGTGILGITAAKLGAQDVICVDIDKKAAEIARENAVI
                     NQVDGRLRIINRDVTTINEPRNLIIANLTAKLLLQLRPHLTQLLLPEGYMIISGIIEQ
                     DTKNIEEHFSVASFIQSKVITEKEWVCYVLRKKSATV"
     gene            2360..3133
                     /locus_tag="VF305_03600"
     CDS             2360..3133
                     /locus_tag="VF305_03600"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX7416250.1"
                     /translation="MTIPRIYSPSALNKEKLCELGPDNYKYLKQVLRLRKGDKIPVFD
                     GFGREFTAVIKNFSSNGVEVELRKRIPTARKGIKITLGQAIPKANKMDLIVKSAAELG
                     ADVIIPFAAARSISRIASEKAPAKVARWQKIVREAARCSRASQITEVTPVVSFAALIA
                     QAGDQSFKMIFWEEESQKSIKDALTDKKLRLINDFFVIVGPEGGFSKDEIIKAKEAGF
                     ISVSLGKQILKVETAAAAIISIIQYEKGIFSNRSKEVNK"
     gene            3130..3732
                     /locus_tag="VF305_03605"
     CDS             3130..3732
                     /locus_tag="VF305_03605"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7416251.1"
                     /translation="MTIYKYAGFWRRLVAYTIDGTIIFFTFIVLFTIVSLAFFSGAIS
                     GDSRAWVADLANPTRFSSIIIFIWIFCISINIAYFTYFHGTTGRTPGKMLLGLQVLSA
                     GGTSISFGTAFLRSVGYFVSSIFYLGFIWVAFDKRKQGWHDKIADTVVIIREQKNQTA
                     GISIPDHSPILPTPQHEGEQVEAFKEPMTQPAKTPGSNNS"
     gene            3832..3907
                     /locus_tag="VF305_03610"
     tRNA            3832..3907
                     /locus_tag="VF305_03610"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3865..3867,aa:Phe,seq:gaa)
     gene            3988..>4218
                     /locus_tag="VF305_03615"
     CDS             3988..>4218
                     /locus_tag="VF305_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416252.1"
                     /translation="MTKRKMKIMLVNPPNCGRSIPEEEYGITSLKQLFKGEPFNLEVL
                     AGPLHGHEVMIFDLKCESEEAFWPAFDAFAPDV"
BASE COUNT         1190 a          958 c          924 g         1146 t
ORIGIN      
        1 aatgtcattg gccgcacctc ataatctttt ccaatttgtt ttccggatgg agagcttctt
       61 ccattctctt aaaaccgagt gggcaggttt tgaggattac aaaactcgat cagaagaaca
      121 aaaaacctcc gatgccgggg tgtcaaatgt cctttcgcat ttttagaact tcgggattga
      181 caaggttggg gggctttcgg ccttccagac cggcaattaa attttccacg gcaagcgttc
      241 ctatttttat acgcgtttcc agagtgccgc tcccaacatg aggaagaagg atcacattgt
      301 ccaactccgt cagaccggga gtaagggaag gctcattttc atagacgtcc agacctgcgc
      361 cgccaatttt ccttgctctt agaacttcca ccagggcttt ctcatcaatc accggccccc
      421 gggatgtatt gatgagaaag gctgtgggct tcatgagtgc caattcactt cttccgataa
      481 ggtgtttagt ttcttctgta agaggcacat gaagggaaac aaagtcagat tctatcagca
      541 gttttttcaa atcgacgtat tccgccatca gggctttttc tccttcgaca tcaattcttt
      601 ttcgattatg atacagaatg cgcatgtcaa atgccctggc ccttgcggca acagctttcc
      661 caattttacc catgccgata attcccagtg tcttacctgt tacttcacgg cctaaaaaaa
      721 gcatcggcgc ccagcattta aaacggcctt ctcttaccat cctgtcacct tcgacaatcc
      781 tccgggaaac ggcaagtatc agggcgaatg caagttccgc cgtcgcatcg gtcaataccc
      841 cgggtgtgtt ggaaaccatg atgccccgtg ccgtggctgc ccgaacatcg atattattgt
      901 aaccgacgcc gaagttggca atcattttta aatgaggagc gcaattcagc acttccccgt
      961 taatggaatc gctgagcatc ggcagaagcc catgtttgtc ttttacacct gaaataatcg
     1021 cttgacgatc cagtggacga tcttcttgat tcatttccac ctggtatttt tctttaagag
     1081 gtgaaatgac attttccgga agatggcctg ttatcaatat ccgcatgttc atgattatta
     1141 ccgattctgt taattatttt tttgcagcat attttataca atatttattg attattcaaa
     1201 agggggattt gcgaaaatga aaatttgttt tatatatgtg gtggttgttt tattgttttt
     1261 tgcagggttt gctgattttg aatgttctcc ggctcttttt tacgctgctt gaaatattac
     1321 cccaaagaag ctatatccat cttcaacatt ggcagaggat tattcatggc agaaaaagaa
     1381 ccggaaaaat ggctaaaaat tgaaatatcg gctcccgctg aacttatgga cgcgctggga
     1441 aatttcctca ctgaaacagg ggcgcagggc gtattccagg aatcctcagc accgcagcat
     1501 accatcgagg attttcctga acctgtaatt gaagaaacac tcaaagccta tttcccgcaa
     1561 gacgcgcgtt gcgaaaaacg aatagctgct gtgcaaaaat atattaacag tctccgggaa
     1621 atattccccg atttcggaaa actatcgttt acaaccgaaa tcatcaacga ccttgattgg
     1681 ggcgaacaat ggaaaaaata tttcaagccc atacgggtaa gcaacaatat catcgtcaaa
     1741 ccgacatggg aaagatattc cccttccagc cgtgacatcg tcattgaaat tgatccgggc
     1801 atggctttcg gtaccggcca acatgcttcc accagaatgt gcatagaagc aatcgaagac
     1861 atcatgaaag accgttcgat taaatcatgg aaagtgctgg acgccggttg cggcaccggc
     1921 atactcggca ttactgccgc caaattgggg gcgcaagatg ttatctgtgt agatattgat
     1981 aaaaaagcgg cggaaatcgc ccgcgaaaac gcggtaatca atcaggttga cggccgcttg
     2041 cgcattatca atcgcgatgt cacaacaatt aatgaaccgc gcaatttaat catcgccaac
     2101 cttaccgcca aattgctctt gcaacttcgc ccgcatctga ctcaattgct tttaccggag
     2161 ggatatatga ttatctccgg catcattgaa caagatacta aaaatatcga agaacatttt
     2221 tccgtcgcgt cgtttatcca aagcaaagtg atcacagaaa aagaatgggt ttgttacgtg
     2281 ctcagaaaaa aatcggcaac tgtttaatag tgaaactttt taaacttaaa cgcctttatt
     2341 aaacaaagga aagttagctt tgaccatccc cagaatttac agcccgagtg ccttgaataa
     2401 agaaaaatta tgcgaactgg ggccagacaa ttacaaatat ttaaaacaag tcttgcgctt
     2461 aagaaaaggc gataagatcc ctgtctttga tggttttggc cgtgagttta cggctgtaat
     2521 taaaaatttt tcttctaacg gggtcgaggt ggaacttaga aaaaggattc caactgcccg
     2581 caaaggaata aaaatcactt tggggcaggc catccccaaa gccaataaaa tggatttaat
     2641 tgttaagagt gccgctgaat tgggcgccga tgtaattatc ccttttgccg cggcgcgttc
     2701 gataagccgg attgcttccg aaaaggctcc agcaaaagtt gcacgctggc aaaagatagt
     2761 tcgggaagca gcgcgttgca gccgcgcgag ccaaataaca gaagtaactc ctgttgtatc
     2821 tttcgctgcc ctgattgcgc aggctggtga ccaatcattc aagatgattt tttgggaaga
     2881 agaatcgcaa aaaagcatca aggacgcact cacggataaa aagctgcgcc tgataaatga
     2941 tttctttgtt attgtcgggc cggaaggcgg tttttccaaa gatgaaatta tcaaggcaaa
     3001 agaagcgggc tttatttctg taagtctcgg caaacaaatt ttaaaagtgg aaacagcagc
     3061 agcagcgata atttccataa tccaatacga aaaaggaatc tttagcaata gatcaaagga
     3121 ggttaacaaa tgaccattta taaatatgct ggtttttggc ggcgtctggt agcatacact
     3181 attgacggca caatcatctt ttttactttt attgtcttat tcacaatcgt gtcgctggca
     3241 tttttctccg gtgctatttc cggagatagc cgcgcctggg ttgccgattt ggccaaccct
     3301 acacgttttt cttcaattat aatttttata tggatatttt gtatctccat caatattgcc
     3361 tattttacct actttcacgg cactacaggc cgcacaccgg gtaaaatgct tcttggcttg
     3421 caggtccttt ccgccggcgg cacatctatc tccttcggca ccgctttttt aaggtcggta
     3481 ggttattttg tctcgagtat attttacctc ggttttatct gggttgcctt tgacaaaaga
     3541 aaacagggtt ggcacgataa gatcgccgat accgtcgtaa taatcaggga gcagaaaaat
     3601 caaactgccg gcatatcaat tcccgatcat tctccaattt tgccgacccc gcaacatgaa
     3661 ggagaacaag ttgaagcctt taaggaacca atgacccaac cggctaaaac accgggaagc
     3721 aataatagct aaatagaggg cagacgggcg caagcggtca aaaaagacct tgacaaaaga
     3781 aatcatattt tatagacgat tgcagccttt gccaggttaa attaatccgg cgggtcggta
     3841 gctcagtcgg tagagcatcg gactgaaaat ccgagtgtcg gcagttcaat tctgccctga
     3901 cccaccatga aactgcaggc cctccctaaa aaggagggct tttttagtcc attatctttt
     3961 aaggtgaggg acctaataag tgtgtccatg acaaaacgaa aaatgaaaat catgctggtc
     4021 aatcctccta actgcgggcg cagcattccc gaggaggaat acggtattac ctcgcttaag
     4081 caactcttca agggagaacc gttcaatctg gaagtcctcg ccggtccttt gcacgggcat
     4141 gaggtaatga tcttcgatct caagtgcgag agcgaagagg ctttttggcc tgccttcgac
     4201 gcgtttgcac cagacgtt
//
LOCUS       DASVIM010000180         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1436030, whole genome shotgun sequence.
ACCESSION   DASVIM010000180 DASVIM010000000
VERSION     DASVIM010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436030"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /gene="tilS"
                     /locus_tag="VF305_03620"
     CDS             <1..708
                     /gene="tilS"
                     /locus_tag="VF305_03620"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF020741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX7416253.1"
                     /translation="RSEDEFIRQHVAHALKSSFIQRQQNRILLKIAYINKLSQAIRWR
                     LLKNILESLNPAKNGVSFIHIKSLDNLVQSCESGKRVALPRGIEARREYDNLILERKK
                     VCSKQIEYEYTMNITGSLYVKERKFTIRSELTTKENVDFSSKNNVYLDLDKIRQPIIL
                     RNRRDGDWFQPLGMQGRQKIKAFFIDHKIPLYKRNEIMLVVDELSVICIENMHFSDRV
                     KITAETKNILKLEIMES"
     gene            1015..>2813
                     /gene="ftsH"
                     /locus_tag="VF305_03625"
     CDS             1015..>2813
                     /gene="ftsH"
                     /locus_tag="VF305_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX7416254.1"
                     /translation="MNQKNIALVLTVLLLSALIWQLFNQPKTAAKDLIYSEMIAFLDK
                     GEISEVTIQGENITGKLANGNVFKTYAPKDDKLVTQFREKGVKITAKPPEESPWYMTL
                     LINFFPIILIIGLWIFMMRQMQSGGGKAMAFGKSKARLITDKSRKVTFADVAGIDEAK
                     AELEEIIDFLKDPKKYTRLGGRIPKGLLLIGPPGTGKTLLARAIAGEAEVPFLSISGS
                     DFVEMFVGVGASRVRDLFSQAKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQ
                     LLVEMDGFESNEGVILISATNRPDVLDPALLRPGRFDRQVIVPLPDVKGREKIFEVHA
                     RKAPIAEDVDFAILARGTPGTSGADIENLINEAVLNAARDNKEKVNMSDFEFAKDKIL
                     MGTERKSMVISDQEKRDTSYHESGHTLVARLIPGTDPIHKVTIIPRGRALGLTQQLPI
                     DEKHTYPQEYLENNIAILLGGRAAEEIILHDFTTGAGNDIERATNLARKMVCEWGMSN
                     KMGPLSYGKKEEQIFLGREFATNRDYSEETAKNIDMEVVSIVKQNYEKAKKLLTDHIE
                     ILHKIAAELLEKEVLNGVEIDALIGNALPNQQQA"
BASE COUNT          886 a          524 c          646 g          757 t
ORIGIN      
        1 cgttcggaag atgaattcat ccggcagcat gttgctcacg ctttaaaatc atcattcatt
       61 caaaggcagc aaaatcgaat tttgttaaaa atagcataca taaacaaatt atcccaggct
      121 atccggtgga gattgcttaa aaatatttta gaaagtttaa atcctgcaaa gaatggggtt
      181 tcctttatcc atataaaatc gctggataat ttggttcaaa gttgcgaatc ggggaaaaga
      241 gttgctcttc ctcggggaat tgaagccaga cgcgagtatg ataatttaat actggagaga
      301 aagaaagtct gctcaaaaca aatagaatat gaatatacta tgaatattac cggctctctt
      361 tatgttaaag aaagaaagtt tactatccgc tcagaactta caacaaaaga aaatgttgat
      421 tttagcagta aaaataatgt ttatttagat ttagataaaa tccggcaacc tataatattg
      481 cgcaatagaa gagatggcga ctggtttcag cccttaggta tgcaaggacg gcaaaaaatt
      541 aaagctttct tcattgatca taagataccg ctgtataaac gtaatgaaat tatgctggtt
      601 gtcgatgaac tctccgttat ctgtatagaa aacatgcatt ttagcgatcg ggttaaaata
      661 accgcagaaa cgaaaaatat tcttaagctg gaaattatgg aatcataaat tctgccttta
      721 ataaaaccat tagaaattat ttgagttatg ttagatatta ttataaaggt catactagtt
      781 cgcttgtaat taccacctta caggcggtaa ttaccggtaa ggtaaatttt tgatttgata
      841 tgcgctcccc ttgcgggatg cgccaccggt cttaggctaa ctttgttggc agcgtcgtcg
      901 gcctcctcaa cgtatgtaaa atacgcctgt ggaacctcct ccttgctgct tcgttatcct
      961 ttgaaatcga aatagtatat cttgatgtta taattaattt taaggagaaa aattttgaac
     1021 caaaagaata tcgctctggt acttactgtg ctgctcttgt ctgcactaat atggcagctc
     1081 tttaatcaac cgaaaacagc cgccaaagac ttaatttata gtgagatgat cgcctttctg
     1141 gataaaggcg agatcagtga agttaccatt caaggggaaa atattaccgg taagttggcc
     1201 aacggtaatg tttttaagac atacgctccc aaagacgata aactggtgac acaatttaga
     1261 gaaaagggcg taaagattac ggccaaaccg ccggaagagt cgccttggta tatgactctt
     1321 ttgattaatt ttttccccat tatcttgata atcggtttat ggatctttat gatgcgccag
     1381 atgcaatccg gtggcggcaa agcaatggcc ttcggcaaaa gcaaggcgcg tttgattacc
     1441 gataaatcta ggaaagttac ttttgccgat gtcgccggta ttgatgaggc caaggccgag
     1501 ttggaagaaa ttatcgattt tttaaaggat cccaagaaat atacaaggct gggcggaaga
     1561 attcctaaag gactgttgct tattggcccg cccggaactg gcaaaactct tttagcccgg
     1621 gcaattgcgg gcgaagctga agtgcctttt ttgagcatca gcggctctga ttttgtggaa
     1681 atgtttgtcg gtgtcggagc ttcacgcgtc cgcgatttat tcagccaggc caaaaagaat
     1741 gcaccctgta taatttttat tgatgaaatt gatgcggttg gccgtcatcg cggcgcaggc
     1801 cttgggggag ggcacgatga aagggagcaa actcttaatc agttgcttgt ggagatggac
     1861 ggttttgaat ccaatgaagg cgtgatactt atttccgcta ccaaccgtcc cgatgtcctc
     1921 gacccggcat tgctgcgtcc gggcaggttt gataggcaag ttattgtgcc gctgccggat
     1981 gtaaaaggca gagaaaaaat ctttgaagtc cacgcccgca aagcgcctat agctgaggat
     2041 gttgattttg caattttggc tcggggaacg cccggaactt ccggagccga tatagaaaac
     2101 cttattaacg aagcagtgct caatgccgcc cgggataata aagaaaaagt caatatgagc
     2161 gatttcgagt tcgctaagga taaaatttta atgggaacgg aaagaaaaag tatggtgata
     2221 agcgaccaag agaagcgtga cacctcttat catgaatcag gccataccct ggtagcgcgt
     2281 ttgataccgg gaaccgatcc gatccataag gttacaatca ttcccagagg cagggctttg
     2341 ggtttaaccc agcagttgcc aattgatgaa aaacatacct atccccaaga atatttagaa
     2401 aataacatcg ctattttgtt aggcggacga gctgccgaag aaataatttt acatgatttt
     2461 accacgggag cgggcaatga cattgaaagg gcgaccaatc tagccaggaa aatggtctgc
     2521 gaatggggta tgagcaataa aatggggcca ttgagttacg gaaaaaaaga agagcaaatc
     2581 tttttggggc gggaatttgc cactaatagg gattacagcg aagaaacggc aaagaatatt
     2641 gatatggaag ttgtatctat tgtcaaacaa aactacgaaa aggcaaaaaa gcttctaacc
     2701 gaccatattg aaatactgca caagatagct gctgaactac tggaaaaaga agttttaaac
     2761 ggagtggaaa tagatgcctt gattggcaat gctttgccga atcagcaaca ggc
//
LOCUS       DASVIM010000181         3663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_497171, whole genome shotgun sequence.
ACCESSION   DASVIM010000181 DASVIM010000000
VERSION     DASVIM010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497171"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /gene="dnaA"
                     /locus_tag="VF305_03630"
     CDS             complement(<1..1113)
                     /gene="dnaA"
                     /locus_tag="VF305_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX7416255.1"
                     /translation="MEAVWDKTSKMIQEKLSKQNFDTWIKPITIIAMEDKCVQLSVPN
                     KFFKDWLVDNYFSTIKQSLQNVVGIEVDVEFVLSKNKEQGTEGATTYTTHVKNVSPSA
                     APKGKNYFFLNNNYTFDRFVVGPCNQFAHAASIAVAKQPAKNYNPLFIYGGSGLGKTH
                     LLNATGLLTTATHPELNVMYVSAEEFMNEMINSIRYDRMPKFREKYRNISCLLIDDIH
                     FLAGKERTQEEFFHTFNTLHDSGKQIVVTSDKFPKDIPNLEGRLRSRFEWGLIADIQP
                     PEIETKIAIIEKKMQENKLDLPSNVAHYIASNVESNIRELEGFLVRISAYSSLTGREI
                     NLDLVKEVLRNLVKRNEKGEVSIEEIIKTVAGILNVK"
     gene            complement(1174..2028)
                     /locus_tag="VF305_03635"
     CDS             complement(1174..2028)
                     /locus_tag="VF305_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7416256.1"
                     /translation="MAQKNVLGKGLGAIFPDLLNNLDKKTRVFSCGIEELRPNRYQPR
                     KNFNDDNQKQLIASVKNSGIIQPIIVRKFDKGYEIIAGERRWRAAQAAGLKDVPIVIR
                     EATDMEAAELSLIENIQREELNPLEEANAYVTLMEKFKLSQEDISSRVGKDRSTIANT
                     VRLLKLPAKVKAALVEKKISSGHARCLLACGSSEEQIAALDLILKKGLNVRETERLIQ
                     NIKKPPASKKQGSKDRFLAALEKSLATKFIAKVHIKGSPQKGTIEIRYTSMDELNRLT
                     GLILDDLK"
     gene            complement(2029..2796)
                     /locus_tag="VF305_03640"
     CDS             complement(2029..2796)
                     /locus_tag="VF305_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7416257.1"
                     /translation="MNKIISIANQKGGVGKTTTAINLSSSLAAAEKKTLLIDGDSQGN
                     STSGMGIESNSFQEANLYHAMIGSAPLEKVILPTAIPHLDIIPATQDLIGVEVEFIHI
                     ENREKRLRNLLKSMERQYDFILIDCPPSLGFMTVNALVAADCLIVPLQCEYFALEGLG
                     YLLNTVKLVKAGLNPELALGGILLTMFDPRNLLSHRVAEDVKKHFGDKVFKTMIPRNV
                     RLSESPSHGLPIILYDIKSRGAVAYMELAQEIIEGGI"
     gene            complement(2869..3537)
                     /gene="rsmG"
                     /locus_tag="VF305_03645"
     CDS             complement(2869..3537)
                     /gene="rsmG"
                     /locus_tag="VF305_03645"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX7416258.1"
                     /translation="MLLLHQNAQRLGIALSDIQLDQFDTYQRELLKWNAKINLISEKS
                     SHEINNRHFLDSLTALQFIDHHDTRVVDIGSGAGFPGIPLKIACPDLQLYLLESNRKK
                     VSFLKHIIRLLSLTTTFVLHNRAESLIKESTWKDFFDIVISRAAFKLPEFLSLGAFFL
                     APQGKLIALKGRDVDSEFSACVKSATASYFDELFQYDINSPLRDTPHKIIVGKRIKKD
                     KKAF"
BASE COUNT         1043 a          743 c          701 g         1176 t
ORIGIN      
        1 ctttacattt aatattccag caactgtttt aattatttct tcaatactta cctctccttt
       61 ttcattgcgc ttaactaaat ttcttaatac ttcctttaca aggtccaaat taatttctct
      121 acccgtgaga gatgaataag cacttattct gactaaaaaa ccttcaagtt cgcgaatgtt
      181 ggactctaca ttagaggcga tataatgggc aacattgctg ggtagatcaa gtttattctc
      241 ttgcatcttt ttttcaataa ttgcgatttt tgtttctatt tccggcggtt gaatatctgc
      301 aatcagcccc cattcgaagc gggaccgaag ccggccctct aagttgggta tatctttagg
      361 aaacttatcg ctggtgacca ctatctgttt tccggaatca tgtaatgtat taaaagtatg
      421 gaaaaattcc tcttgagttc tttcttttcc cgccaaaaaa tgaatatcat ctattaatag
      481 acagctgata ttgcggtatt tttccctaaa tttaggcatc cggtcataac ggatggagtt
      541 tatcatttca ttcataaatt cttcggcgga aacatacata acatttagtt ccggatgagt
      601 tgcagtagta agcagtccag tggcgttaag caagtgagtt tttcctaagc ccgatccgcc
      661 ataaatgaaa agaggattat aatttttagc cggttgttta gctacggcaa tggaagccgc
      721 gtgggcaaat tgattacacg gaccgaccac aaacctgtca aaggtataat tattattaag
      781 aaaaaagtaa tttttacctt taggggccgc tgaaggtgaa acatttttca cgtgagtcgt
      841 gtatgttgtc gcaccttctg tcccttgttc tttatttttc gagaggacaa actcgacatc
      901 aacttcaata cctacaacat tttgcaatga ttgtttaatc gtggagaaat aattgtctac
      961 aagccaatct ttgaaaaatt tattaggtac tgataattgc acacatttgt cttccatggc
     1021 aataattgtg attggtttta tccatgtatc aaaattttgc ttacttagct tttcctgaat
     1081 cattttactg gttttatccc agactgcttc cacaatacaa tcctttataa acaggcttat
     1141 caacaggtgt tgacaagcca tgaagttgaa gtatcatttc aaatcgtcga gaatcaatcc
     1201 tgttaatcga ttcaattcat ccatggaagt ataccgaatt tcgattgttc ctttttgggg
     1261 tgagccctta atgtgcacct tagctataaa ttttgttgcc aacgactttt ccaaagcggc
     1321 caaaaagcgg tctttagaac cttgtttttt actagctggc ggctttttta tgttttgtat
     1381 taatcgttcc gtttcccgaa cgtttaatcc ttttttcaaa attaaatcca gcgccgcaat
     1441 ttgttcttcg gaggagccac aagccaataa acagcgggca tggcctgaag aaatcttctt
     1501 ttctaccaga gctgctttga ctttagccgg taatttcaaa agacggacgg tattggcaat
     1561 tgtcgaacga tctttaccaa cgcgagaaga aatgtcttct tgagaaagtt taaatttctc
     1621 cattaacgtt acataggcgt ttgcttcttc cagcggattt aattcttccc gctgaatgtt
     1681 ttcaatcagc gagagttccg ccgcttccat atctgtcgct tcacgaatga caatgggtac
     1741 atcttttaaa ccggcagcct gagctgcccg ccagcggcgc tccccggcaa tgatttcata
     1801 ccccttatca aatttgcgga caataattgg ttgaataata ccgctgtttt taactgaagc
     1861 aataagttgc ttttggttgt cgtcattaaa gttttttcgg ggttgatagc ggtttgggcg
     1921 caactcttca ataccacaag aaaaaacacg tgttttcttg tctagattat tcaataaatc
     1981 cggaaaaatg gcaccgagcc ctttacctaa tacatttttt tgtgccattt atatcccccc
     2041 ttcaattatt tcttgagcca gttccatata agcaacggcc ccgcgcgatt taatgtcata
     2101 aagaataatt ggtaagccgt ggctggggct ttccgaaagc cggacattgc gcgggatcat
     2161 cgttttaaaa actttatcac caaaatgttt ttttacatct tcagcgacac gatgcgatag
     2221 taaattacgc ggatcaaaca tagttagcaa aataccgcct aaggctaatt ccggatttaa
     2281 tccggctttg accagcttca ccgtgttgag taaatatcct aatccttcca gagcaaaata
     2341 ctcgcactgc agaggaacaa tcaaacagtc ggcagcgaca agggcattga ccgtcataaa
     2401 gcctagtgat gggggacagt caattaatat aaagtcatat tgcctttcca tagattttaa
     2461 caaattgcgc agacgctttt ccctgttctc aatgtggatg aactcaacct caacaccaat
     2521 taaatcctga gtcgcgggaa ttatatccag atgaggtata gctgtgggca gaataacttt
     2581 ttctaagggc gctgagccga tcattgcatg atataaatta gcttcttgaa aagagttact
     2641 ctctatgccc atgccgctgg ttgaattacc ctgcgaatca ccatcaatga gcaaagtttt
     2701 tttttccgca gcggccagag acgaagacag attaatcgcg gtagttgttt tgccgacccc
     2761 tcctttttga ttggctatgc ttataatttt attcattttt ttatttgatg tcttcctgga
     2821 aattcttttg aaaaaactgc cgtaagaagt agcataaaaa aatttttttt agaaggcttt
     2881 tttatctttt tttatccttt tccccacaat aattttatgc ggagtatccc gcaggggcga
     2941 atttatatca tattgaaata attcatcaaa ataagatgct gtagcgcttt tgacacaggc
     3001 ggaaaattca ctatcgacat cacggccttt taaggcgatc aatttacctt gtggagcaag
     3061 aaaaaaagcg cccagcgata ggaattcggg tagtttaaag gcggcacggg aaattactat
     3121 atcgaaaaaa tctttccaag tactctcctt tattaagctt tcagcgcggt tatgaagaac
     3181 aaaagttgtg gtaagggaaa gcaggcggat aatatgcttt aaaaaggaaa cttttttgcg
     3241 attgctttcc agaagataga gctgcaagtc cggacaggcg atttttaagg gaattcccgg
     3301 aaatccggcg ccgcttccta tatcaactac ccgggtgtca tgatggtcaa tgaattgcaa
     3361 cgccgttaat gaatccaaaa aatggcggtt attgatttca tgggaagatt tttccgaaat
     3421 taaatttatt ttggcattcc atttcagcaa ttctcgttga taggtatcaa attgatctaa
     3481 ctgaatatcg ctcaatgcaa tacctagccg ctgcgcattt tgatgaagca aaagcatttc
     3541 cttatccatg atcagggaat aacaaaaaat atgtgccgcc gcaaacgata aattatccgt
     3601 gcatttcttt tattacagct ttatagagca ggtagtgtat ttttaggagg gcaaatgttg
     3661 tcg
//
LOCUS       DASVIM010000182         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_413653, whole genome shotgun sequence.
ACCESSION   DASVIM010000182 DASVIM010000000
VERSION     DASVIM010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413653"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /gene="serS"
                     /locus_tag="VF305_03650"
     CDS             complement(<1..1199)
                     /gene="serS"
                     /locus_tag="VF305_03650"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX7416259.1"
                     /translation="MLDIKYLRQNIEFVRKKMDERKQPIDFDRFLILDAKRRDILLEV
                     ETLRNERNDASKKIGEFKKKKADASTLIAKMSEVSAKIKELDENLRITEEELNNFMLM
                     IPNIQHESVPVGQGSEDNPILRSWGKKPAFAFEPKAHWDIGESLNILDFARGAKIAGA
                     RFTLYRGLGAALERALINFMLDLHTGEHGYTEVLTPFMVNRESMTGTGQLPKFAEDLF
                     KIEGMDYFLIPTAEVPVTNIFRDEILEENKLPVNYVAYSPCFRSEAGSYGKDTRGLIR
                     QHQFNKVEMVKFVKPEDSYDELEKLTVNAEEVLKRLGIHHRTVTLCSADLGFSSAKTY
                     DVEAWMPGQNVYREISSCSNFEYFQARRASIRFRREENGKVEFVHTLNGSGLAVGRTV
                     VTILENY"
     gene            complement(1300..>2824)
                     /gene="murJ"
                     /locus_tag="VF305_03655"
     CDS             complement(1300..>2824)
                     /gene="murJ"
                     /locus_tag="VF305_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX7416260.1"
                     /translation="ISTMASRIFGYIRDMVVASFFGASWLTDAFWVAFRIPNLLRRLL
                     GEGSLTVSFVPVFTEYLQKKSRQEALELVSIAFTLLSIILVIISILGVIFSPFIVTLM
                     APGFIKEPGQFALTVYLNRLMFPYIFFISLVALCMGILNSFRHFAAPALSPVILNISM
                     ILAALFLRNYFEEPITALAVGVMIGGVLQLAMQWPFLVKYGVKFKFKFNFRHPGIKKI
                     GLLMFPAFFGAAVYQIDVLVGMILASLLPSGSVSYLFYADRIMELPLGVFAVAIGTAS
                     LPSFSEHVSKGNFEELKSSLSFSLRLMLFVTIPSMVALMALHFPVISVLFQRGAFNVQ
                     AVISTSQALFCYALGLWAFSLVRVIVPAFYSLQDAKWPLKAAIIAVVFNVIASLILMN
                     PLKHSGLALANSLASMVNVIILSIVLKKKIGTFLDRNFYLSTGKIIISSLVMLGAIIL
                     VDFFLPWNTDAHFKARLIYLVVSIAVGGSAFFICSYLLKSPEIQSIVRLLKKRFSRQ"
BASE COUNT          897 a          670 c          576 g          681 t
ORIGIN      
        1 tgataattct ctaaaatcgt caccacagtt ctacccacgg ccaggccaga accgtttaat
       61 gtgtgaacaa attccacctt accgttttct tcccggcgaa aacggatcga agcccggcga
      121 gcctgaaaat attcaaagtt actacaggaa gagatttcgc gatatacatt ttgccctggc
      181 atccaggctt ccacatcata ggttttagcg gatgaaaacc ccaaatccgc agagcatagg
      241 gttaccgtac gatgatgaat tcccaatctc ttgagaactt cttccgcatt gacggttaat
      301 ttttccagtt catcataaga atcttccggc ttgacaaact tgaccatttc caccttgtta
      361 aactggtgct ggcgaattaa gccccgcgta tccttgccgt aagagccggc ctccgaacgg
      421 aaacaaggcg aataagccac ataattaacc ggcagcttgt tttcctccag tatttcatca
      481 cggaaaatgt tcgtaacggg aacttccgcg gtcggaatca aaaaataatc cattccttca
      541 attttaaaaa ggtcctcggc aaatttcggc aactggccgg taccggtcat actttcccgg
      601 ttaaccatga atggcgtcag cacttcggta taaccgtgct cacctgtatg taagtcgagc
      661 atgaaattga tcaatgcccg ctccaacgcg gcgcctaatc cccggtataa agtaaaccgc
      721 gctccggcta tttttgcccc gcgcgcaaaa tccaaaatat tcaggctctc tccgatatcc
      781 cagtgagctt tcggctcaaa agcaaaggcc ggcttttttc cccaactgcg caggatagga
      841 ttgtcttccg agccttggcc aacgggcacc gattcatgtt gaatattagg aatcatgagc
      901 atgaaattat tgagctcttc ttcggtgatt cttaagttct catcaagctc cttgatttta
      961 gccgaaactt cactcatctt ggcaatcaat gtggaggcat cggctttctt tttcttgaac
     1021 tcgccgattt ttttggaagc gtcattacgc tcgttgcgca gtgtttctac ctccaataaa
     1081 atatctcttc tttttgcatc tagaataagg aagcggtcga aatcaatcgg ttgtttcctt
     1141 tcgtccatct ttttgcggac aaactcgata ttttgcctta aatatttaat atccagcatg
     1201 tattcccctg ccaaatctac ctaaacaaac aattataaag acgaaagcac ctatcacttt
     1261 gagcgggtga agtcaatgat tttgtccgcc aaatcaatac tattggcggc taaatctctt
     1321 ttttaacagt cggacaatgc tctgaatttc ggggcttttc aaaagatatg agcagataaa
     1381 aaatgcagac ccgcccacgg caatcgaaac aacaaggtaa atcagccgcg ccttaaagtg
     1441 tgcgtccgtg ttccagggca aaaagaaatc aaccagtatt attgctccca acataactaa
     1501 cgaagaaata attatcttgc cggtagataa ataaaagtta cgatctaaaa atgtcccgat
     1561 ttttttcttt aaaacgatgc tcaaaataat tacattgacc atcgaagcca atgagttggc
     1621 cagcgccagg ccactgtgtt tcaggggatt cataagaata aggctggcaa taacgttaaa
     1681 gactacagca ataatggccg ctttcagtgg ccatttggca tcttgcaaag aataaaaagc
     1741 gggaacaata acacggacta acgaaaatgc ccacaaaccc aaagcataac aaaataatgc
     1801 ttgcgaggtg gaaattacgg cttggacatt aaaagcaccc cgttgaaata aaactgaaat
     1861 aaccggaaaa tgcaaagcca ttaaggccac catcgaggga attgtcacaa aaagcatgag
     1921 ccgcagagaa aaagaaaggc ttgattttaa ttcttcaaaa tttcccttcg agacgtgctc
     1981 ggaaaagctc ggcagcgatg cagtcccgat agcgacggcg aaaacgccca aaggcaattc
     2041 cataatgcga tcggcataaa aaagataaga cacactgccg ctgggcaaaa gcgacgcgag
     2101 gatcatgccg acaagaacat caatctgata tactgctgcg ccaaaaaatg ccggaaacat
     2161 caaaaggccg atcttcttaa tccccggatg acgaaaattg aacttaaatt taaattttac
     2221 tccgtattta actaaaaagg gccactgcat tgctaactgc agaacgccgc cgatcatcac
     2281 accaacagcg agtgctgtaa tcggctcttc aaaataattg cgcaaaaaca gcgccgccaa
     2341 aatcatagag atattcaaaa ttaccggtga taaggccggg gcggcaaaat gccggaaaga
     2401 attcaaaata cccatgcaca aggccactag cgaaatgaag aagatgtagg gaaacatcag
     2461 gcggttcaaa tagacagtca gggcaaattg gccgggttct ttaataaatc ccggcgccat
     2521 tagagtaact atgaacggtg aaaaaataac gccaagaatt gagataatca ccaaaataat
     2581 ggatagaagg gtaaaggcaa tagataccaa ctccagagct tcttgccttg attttttttg
     2641 gaggtattcg gtaaaaacgg gaacaaaaga aacagtgagc gatccctcgc cgagcaaccg
     2701 tcttaaaagg ttgggaatac gaaaggcaac ccaaaaagcg tctgtcaacc agctggcgcc
     2761 aaaaaaactc gcgaccacca tatcgcgaat atagccaaat atccggctcg ccatcgttga
     2821 tatc
//
LOCUS       DASVIM010000183         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1522675, whole genome shotgun sequence.
ACCESSION   DASVIM010000183 DASVIM010000000
VERSION     DASVIM010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522675"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VF305_03660"
     CDS             <1..731
                     /locus_tag="VF305_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7416261.1"
                     /translation="NNQKSFVQQLYLRKSLIFMAAIKIKGISIETIIFDFDGTLAKLN
                     IDFKLIRNAVLKLISSYGIKRNYLQTDFVLEMINTSKEILSKSSPLQAEIFANKAIQM
                     IEAMEIEAAINGELFPQTKELFTRLKLSGISTGVITRNCEKAIKTVFPDILSYCEVVV
                     CRNIVNNVKPHPEHLNKALQMLNSLAENTLMIGDHPLDIQTGRNAGTFTAGVLTGHFS
                     KSDFLNAGADLVLSQASDLLNMIN"
     gene            997..2892
                     /locus_tag="VF305_03665"
     CDS             997..2892
                     /locus_tag="VF305_03665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7416262.1"
                     /translation="MNLENSQKYLAACKKAYPEKFASENEIFNHIRRGDHIFVASGCG
                     EPQYLVQAMTSYVESHPKAFFDAEIIHVYSLGIAPYTDAKYKANFRHNSFFIGNNTRD
                     PINKGMADYTPISLSHVPSLLYSGVIRIDVALIQTSLPDDHGYFSLGVSVDIVKAATE
                     KAAIVIAQVNANMPRIHGDSFLHIKDVDFIIPYDEPLLELTGAKTSETIQRIGKNVAR
                     LVQDGDTIQVGFGSLPNAIMANLYNKNNLGVHTELLSDGLVDLIKAGVIDNSRKTIDR
                     GKTTAAFSMGKKATYDFLNDNPSIAFRTIDYTNNPLVIAQIENMVAINSALEIDLTGQ
                     ATSESIGSIFYSGVGGHQDFMRGALLSKNGKTVLAMKATSRDDTISRIVPALKEQAGV
                     TLNRSDVRYIVTEYGIAYLHGKNIRERAMELISIAHPKFRPWLIEEAKKRGLIFKDQA
                     FIPGKHGEYPAELETFRTTKTGLQIFIRPVKISDEPLLQDFFYSLSDTSLHKRFLSFR
                     QDMPHERLQDFVVIDYTKEVLLLVITGDQESQLIAAVGQYGIDENSHTAEVAFAVRDD
                     QQNRGIGFELLKYLTYLARRQGLLGFTAEVLVDNKPMLHVFEKGGFDMKKRSDSGVYD
                     LMLKFKI"
     gene            2918..>3115
                     /locus_tag="VF305_03670"
     CDS             2918..>3115
                     /locus_tag="VF305_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEX7416263.1"
                     /translation="MNPNVFREYDVRGVVDKDLNEEFVFNLGRAIGTYAARHKIKNMT
                     IGRDCRLSSNDYHHFLIKGLNH"
BASE COUNT          963 a          643 c          590 g          919 t
ORIGIN      
        1 agaacaatca aaaaagtttt gtccaacagt tatatctcag gaaatcgctg atctttatgg
       61 ctgcaataaa aattaaaggt atttccatag aaactataat cttcgacttt gatggaactc
      121 tggcaaaatt aaatattgat tttaaactga tccggaatgc tgttttgaaa cttatttctt
      181 cttacggcat caagcgaaat tatctgcaaa cagattttgt tttagaaatg attaacacat
      241 ccaaggaaat attaagcaag agttcccccc tccaagctga aatatttgct aataaagcaa
      301 tccaaatgat tgaagctatg gaaatagaag cagctatcaa tggcgaatta tttccccaaa
      361 caaaagaatt attcacccgt cttaaattat ccggaatttc tacgggagtg ataaccagaa
      421 attgcgagaa ggccataaaa actgtattcc ccgatatctt atcttattgc gaggtggttg
      481 tctgtcgcaa tatcgtgaat aacgttaaac ctcatcctga acatttaaat aaagccctgc
      541 agatgctaaa cagcttggcc gaaaatactt tgatgattgg tgatcatcct ttagatattc
      601 aaaccggtcg taatgcggga acgtttacag cgggtgtact caccggacac ttcagtaaat
      661 ctgatttttt gaatgccgga gctgatctag ttttatccca agccagcgat ctattaaata
      721 tgataaatta attttcgcct aaacccggat gagttgagct tcttcagcac taattcccgt
      781 tagttttatt ttctgccaac ttaccgttat tttttgcatt atctttattt gacaaaaagt
      841 atcattttaa cctattatta aattaaagat atcgattctc gtcggcaaac ctgccataga
      901 gaatctctta aatgttgatt taatttcatt tttaatctaa atactaactg tgaatagtat
      961 cgcctattat aatttaatta attagctaag acgattatga atttagaaaa ctctcaaaaa
     1021 tatcttgccg cctgtaagaa agcgtaccct gaaaaattcg cttccgaaaa tgaaattttt
     1081 aatcacattc gccgcggcga ccatattttt gttgcctccg gatgcggaga accgcaatat
     1141 cttgttcaag caatgaccag ctatgtcgaa tctcatccca aagccttttt tgatgcagaa
     1201 attatccatg tctattcttt aggcattgct ccctatactg acgccaaata taaagcaaac
     1261 ttccgccata actctttttt tatcggtaac aatactcgcg atcccattaa caaaggtatg
     1321 gctgattata cccctatttc tctttcgcat gttccttcat tattatatag tggtgtaatt
     1381 cgcattgacg tagctctaat acaaacttct ctgcccgatg atcatggcta ctttagcctt
     1441 ggcgtcagcg tggacatagt aaaagccgct acggaaaagg cggcaattgt tattgctcaa
     1501 gtaaacgcca atatgccccg aattcacggt gatagttttc ttcatattaa agatgtagat
     1561 tttattattc cctatgatga gccgctcttg gaactaacag gcgcaaaaac aagcgaaacc
     1621 attcaaagaa tcggtaaaaa cgtagcgcgc ttagtgcagg atggcgacac cattcaagtg
     1681 ggattcggca gcctgcccaa tgccataatg gccaatttgt ataataaaaa taatctgggc
     1741 gttcatacag aacttttaag cgatggtctg gtggatttaa tcaaagccgg tgttatcgac
     1801 aattccagaa aaaccatcga ccgcggaaaa acgactgccg ctttcagcat ggggaaaaaa
     1861 gccacttatg atttcctcaa tgacaatcct tccatagctt tccggacaat tgactacacg
     1921 aataatccgt tagtcattgc ccaaatagaa aatatggtcg ctattaatag cgctttggaa
     1981 atagacctca ccgggcaggc cacttcggaa tcgattggca gcatttttta tagcggcgtc
     2041 ggcgggcatc aagatttcat gagaggagca ttactttcca aaaacggcaa gaccgttctc
     2101 gctatgaaag ctacttctcg tgacgacact atttcccgca tcgttccggc tctgaaagaa
     2161 caagcgggag tgactctcaa ccgtagtgat gtccgctata tagtaacgga atatggcatt
     2221 gcgtacttgc atggaaaaaa catccgggaa cgcgctatgg aattaatttc tatagctcac
     2281 cctaagttcc ggccatggct gattgaagaa gccaaaaaac gtggcttgat ttttaaagat
     2341 caagctttta ttcccggaaa acacggtgaa tatcccgcag agttggaaac gttccgtacc
     2401 acaaaaacgg gattacaaat attcatcaga ccggttaaaa taagtgatga acctcttctg
     2461 caagattttt tttattccct gtccgatacg agtttgcaca aacggttcct ctcttttcgt
     2521 caagacatgc cgcatgaaag actgcaggat tttgttgtta ttgattacac aaaagaagtt
     2581 ctcctgttag ttatcaccgg agatcaagaa agccagctca tagccgcagt cgggcaatat
     2641 ggtatagacg aaaacagcca tactgctgaa gttgcgtttg ctgtgcgcga cgatcagcaa
     2701 aaccgcggta tcggctttga attgttaaaa tacctcactt atcttgccag aagacaggga
     2761 ttacttggtt tcactgcgga agttcttgtc gataataagc ctatgcttca tgttttcgaa
     2821 aaaggcggat ttgacatgaa aaaaagatct gatagcggcg tttacgattt aatgcttaag
     2881 tttaaaatat aacttatctt agaaagattt atatttgatg aatcccaatg tatttcgtga
     2941 atatgacgtt agaggtgttg tggacaaaga cttaaacgaa gaatttgtct tcaaccttgg
     3001 tcgggctatc ggcacttatg ccgcccggca caaaataaaa aacatgacca tcggacggga
     3061 ttgccgttta agctctaatg attatcacca tttcttaatc aaaggcctta atcac
//
LOCUS       DASVIM010000184         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1522900, whole genome shotgun sequence.
ACCESSION   DASVIM010000184 DASVIM010000000
VERSION     DASVIM010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522900"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1753
                     /locus_tag="VF305_03675"
     CDS             <1..1753
                     /locus_tag="VF305_03675"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX7416264.1"
                     /translation="EKEKGITVRNLNTQKELEIKDISKILNTQEIDAALLKRTNAIFN
                     DDDYATRSVIFSLAKKLIEPNLTFNKETTEKKKLAAMEEVKPVLFQVQTNEMIVREGE
                     KIGYLELAKLDAFFKSTGNNKFSSVAVLAGIFFTALFLSLVLYFWRTRNWLKPSIRSN
                     VDLLVFGLIALLQIFFIKTGIFISLAINRAFPSISTGACYFAIPFALGAMIIAVLMNR
                     NVALIISVFTSFLIAFLFDEKIIFPLFSFLGSIAASYQIVRSRQRSAFLRVGIFLGII
                     NMAAIACINLLSGNLLNDLFLRLVMGFAGGILTGILVAGITPVFESIFGFITDIKLLE
                     LANLNQPLFQKMIIEAPGTYHHSIIVASLVEAAAEAIGANALLAKVSAYYHDIGKLTK
                     PQYFIENQPNNENKHDKLSPKMSSLIIISHVKDGCELSSEVKLGQQIINIIREHHGTS
                     IVSYFYDKAKKNKDESIRSLSESDFRYPGPKPQTKEAGLVMLGDVIEASSRTLSNPTP
                     ARIHSLVRERIERIYMDGQLDECELTLRNLNTIAETFTKILTGIFHHRVDYPESAHKE
                     TNGKKEIDELTDPKQSA"
     gene            1722..2180
                     /gene="ybeY"
                     /locus_tag="VF305_03680"
     CDS             1722..2180
                     /gene="ybeY"
                     /locus_tag="VF305_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX7416265.1"
                     /translation="MNLQIQNNQRKIKIDRRKIRSTIFKILKILDCADKEISLSFVDD
                     EKIKQLNKQYLGKDKATNVLSFSLREDEYGNINPQILVDIVISADTAQKDAIQGNLTI
                     AQEIDFLIIHGLLHLLGYNHENTTKKETNIMRQKEKKLFNLVNGNDIKLI"
     gene            2333..3085
                     /locus_tag="VF305_03685"
     CDS             2333..3085
                     /locus_tag="VF305_03685"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX7416266.1"
                     /translation="MELSLKNKVVLITGASRGIGQAAAIGLAKAGADLAIASRKLPDL
                     EKVAAEIKKLGRKCVPIAAHVGRIEEINSLVKKTLDEFGKIDILVNNAATNPTMASAM
                     DTDERAWDSIMNLNLKGLFFLSQTVARTMKEKGGGKIINIASIAGISPDILAVYSISK
                     AGVIMATKVMAQQWGQYNIRVNTIAPGLTKTKFSEALWGNPDILNIAMSRTPLARPAE
                     PEEMVGAIIFLASDASSYITGQVIAVDGGTTI"
     gene            3306..>3554
                     /locus_tag="VF305_03690"
     CDS             3306..>3554
                     /locus_tag="VF305_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX7416267.1"
                     /translation="MASDAAYADIYKYVDNEGVLHLTNVPSIPRAKYVLILREKRILF
                     QHNIDINKYDQIIIKAADKFKINSALIKAIIKAESNFNH"
BASE COUNT         1134 a          729 c          693 g          998 t
ORIGIN      
        1 agaaaaggaa aaaggcatta ctgtaagaaa tttaaatact caaaaagaac tagagattaa
       61 agatatttcc aagatattaa atactcagga aatagatgcg gctcttttaa aaagaacaaa
      121 cgctattttt aatgatgacg actatgctac cagaagcgtt atcttttcgc tggccaaaaa
      181 attaatcgag ccgaatctta cttttaataa agaaacaaca gagaagaaaa agcttgccgc
      241 catggaagaa gtaaagccgg tacttttcca agtgcaaaca aatgaaatga ttgtgcggga
      301 aggagaaaaa atcggctact tggagttagc taagttagat gccttcttta aatccaccgg
      361 aaataataaa ttttccagcg ttgcggtact ggcgggaatt ttcttcacag ctctctttct
      421 ttccttagtt ttgtacttct ggcgcaccag aaactggctt aaaccttcaa tacggtccaa
      481 tgtcgatttg cttgtttttg gccttattgc cctattacaa atatttttta ttaaaacagg
      541 aatattcatt tctctggcta tcaatagagc atttccatct atatcaaccg gtgcctgtta
      601 ttttgctata ccctttgcgc tgggagcaat gattatagct gttttaatga atagaaatgt
      661 tgccttaatt ataagcgtgt tcacatcttt cttgattgct tttctttttg atgaaaaaat
      721 tattttccct cttttctcct ttttaggatc aattgccgcg tcttatcaaa ttgtccgcag
      781 ccgtcagcgt tccgcttttt taagagtcgg catctttttg ggaattataa atatggcggc
      841 gattgcttgc atcaaccttc tttcgggaaa tttgctcaat gatttgtttt tacggctggt
      901 gatgggattt gccggaggaa ttcttactgg aatccttgtc gccggaatca ctcctgtttt
      961 tgaaagcatc ttcggcttca taaccgatat taagctgctg gaactggcca atctcaatca
     1021 acctcttttt caaaaaatga ttatcgaagc tcccggaact tatcaccata gcattatcgt
     1081 ggcctcgctg gttgaagctg ctgctgaagc tatcggcgct aatgccttgc tggccaaagt
     1141 cagcgcttat tatcacgata ttggtaaact gacaaaacct caatacttta ttgaaaatca
     1201 acctaataac gaaaataagc acgataaact ttcgccgaaa atgagcagcc tgattataat
     1261 ctctcacgtg aaagatggtt gcgaattatc ctcggaagtt aaactgggac agcaaattat
     1321 aaacataatt cgcgagcatc acggcacaag catcgtcagt tacttttacg acaaggctaa
     1381 aaaaaacaag gatgaatcaa tccgttcttt gtcggaaagc gattttcgtt atccgggtcc
     1441 aaaaccgcaa acaaaagaag ccgggctggt aatgctgggg gatgttattg aagcttcgtc
     1501 acgcaccctt tctaatccga caccggcacg aatccattcc ctggtgcggg aaagaattga
     1561 acgtatctat atggacgggc agcttgacga atgcgagttg actttaagaa atctcaatac
     1621 aatagcggaa acctttacga agattcttac cggaattttc caccaccgtg tcgattaccc
     1681 tgaatctgcg cacaaagaaa caaacggcaa aaaagaaatt gatgaactta cagatccaaa
     1741 acaatcagcg taaaattaag attgatcggc gtaaaattcg cagtacgatc ttcaaaatct
     1801 taaagattct cgattgcgcg gataaagaaa tcagcctttc ttttgttgac gacgaaaaaa
     1861 ttaaacaatt aaacaaacaa tatctgggaa aagataaggc aaccaacgtt ctttcttttt
     1921 ctttgcggga agatgaatac ggcaacatca atccccagat tttagtagac attgttattt
     1981 ccgcagatac tgcccaaaaa gacgccatcc aaggcaatct tactattgcc caagaaattg
     2041 attttctcat cattcatggc ctcttacatc tgttgggcta caatcatgaa aatacgacta
     2101 aaaaagaaac aaacataatg cggcaaaaag agaaaaagtt gtttaatctg gtaaacggca
     2161 acgatataaa gctaatttga taaagcctct tgtctgcctg ctcttttctt cccttgacag
     2221 gtatttttat ttttcttata catatcgcga tccaaaacga gccattacta ctaacaatat
     2281 aaaccaaatt atgaagctgc aaaaactgac tacttacagg aggtaacaaa caatggaatt
     2341 atctttaaaa aataaagttg ttctaattac cggagccagc cgtggaatcg gtcaggcggc
     2401 ggcaataggt ttagccaaag ccggcgccga tctggccata gccagccgca aacttcccga
     2461 tctggaaaaa gtagcggcag aaataaaaaa attaggaagg aaatgcgtgc ctatagccgc
     2521 ccatgtcggc aggatagaag aaataaactc tctggttaag aaaacgttgg atgagttcgg
     2581 gaaaatcgac atcctggtaa ataatgccgc cacaaatccc accatggctt cggctatgga
     2641 cacagacgag cgcgcctggg attcaattat gaacctgaac cttaaagggc tattcttctt
     2701 gagtcagacg gtagccagga caatgaagga aaaaggcgga ggcaaaatta tcaatattgc
     2761 ttctatcgcc ggaatttctc ccgatatatt ggccgtttac tccataagta aagcgggggt
     2821 gatcatggcc acaaaggtaa tggcccaaca atggggtcaa tataatatcc gggttaacac
     2881 catcgccccg ggtttaacca aaaccaaatt cagcgaagca ctttggggaa atcctgacat
     2941 tcttaatatc gccatgagca gaactccgct ggcgcggccg gccgaacccg aggaaatggt
     3001 cggggccatc atttttctgg cttccgacgc atcgagttat ataacagggc aggttattgc
     3061 cgtagacggc ggaactacta tctagcacca atgatgattt gtccagcggt gttttccaga
     3121 taatattgcc attgcattga tgtaattaca ttacacattt taatcagttt ttgttgaaaa
     3181 aaaggcttga agtaaaattg ccgataaagt ataatgtaac catgcaaaat gattcaaaca
     3241 tatacttttg cggcgaaaat ttgttcctgc aaaccgttat tacggtttta gctgtattca
     3301 taattatggc aagtgatgct gcctatgccg acatttacaa atacgtggat aatgaaggcg
     3361 ttctacattt gactaacgta ccttcaattc ctcgcgctaa atatgttctc attctcagag
     3421 agaaacgaat tcttttccag cacaatattg atataaacaa atacgatcaa attattatca
     3481 aagctgccga taaatttaaa attaattcgg ccttaattaa ggcgatcatc aaagcagaat
     3541 ctaactttaa tcat
//
LOCUS       DASVIM010000185         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1011133, whole genome shotgun sequence.
ACCESSION   DASVIM010000185 DASVIM010000000
VERSION     DASVIM010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011133"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1333)
                     /locus_tag="VF305_03695"
     CDS             complement(134..1333)
                     /locus_tag="VF305_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7416268.1"
                     /translation="MDLSLDENQLMYLDTVRKLVKNGITPYILEMEKAHKFPWVIINK
                     AWGMGIINLCIPESIKGFEIDVISSALIIEELSYGDTGISTSAMCNDLANVVIGQHGT
                     DEQKELFLRPFVEKPLLASFCLTEPNAGSDNSMMTTFISKKDDGSYILNGSKCFITNA
                     SYASQYTVLCKVGKPTSNFMACVIVPVEPVTAVDIPDIGQATREINLPTGGKIIIGKP
                     EDKLGQRLSNTASVTFEDVIIQPNQIIGDRRFGFKYIVDVLDYARPMVAAIGLGLARR
                     ALTVTMEYTRERKQFGQRICDLPVAREMLTTMWKKVELAELALLKAVCKIKEKANDAG
                     IYASLAKNTAAEAAIFCSTEGLHLHGGYGFTTEYEISKLARDAHIIDIYEGVREVQNM
                     IIGREIV"
     gene            complement(1437..>2078)
                     /locus_tag="VF305_03700"
     CDS             complement(1437..>2078)
                     /locus_tag="VF305_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416269.1"
                     /translation="ATGMDLHKLLVLGEHLENIAPKYNHHLANHKLSIIDNGVLAHQI
                     PGGMISNLTGQLKEMNALDRLGEIYKEVVQTRKDMGYPPLVTPVSQIIGAQAVLNVLF
                     GRYVRVSNQLKDLVLGLYGKTPVPIDSELTKKILKNYKHGQEPVSGRPADYLEPEIEA
                     AKKLIGNLARNDEDVLAYILYPATMEKFLRTKYGLDSPVSAGVKNFPKKKVAA"
BASE COUNT          577 a          493 c          384 g          624 t
ORIGIN      
        1 gtccggcgat tttggatacc cacacgctcc aaaatccatt cgttatttgt gccgatatcc
       61 aaatcttcta aaaatttgtt cgtaattata ttttccggat tgaaatgtcc catggagtga
      121 agataaagca tttctaaaca atctcccgcc cgataatcat attttgcact tcgcgcacac
      181 cctcataaat atcaataata tgggcgtcgc gcgcgagctt ggaaatttcg tattccgtgg
      241 taaaaccata accgccgtgc aaatgaagac cttccgttga acaaaatata gccgcttctg
      301 cggccgtgtt tttcgcgagc gatgcataga ttccggcatc gtttgctttt tccttaatct
      361 tacagacagc tttcagaaga gcaagttcag ccaattctac ttttttccac atagtggtta
      421 acatttctct ggccacgggt agatcgcaga tcctctgacc aaattgttta cgttcgcgcg
      481 tgtattccat cgtgactgta agcgccctcc tggccaaacc caaacctatt gcggccacca
      541 tcggccgggc ataatccaaa acgtcgacaa tatacttaaa accgaatcgc ctatccccaa
      601 taatctgatt aggttgaata ataacatcct caaaagtgac gctggctgtg ttcgaaagtc
      661 tttgtcctaa tttatcttcc ggcttgccga taattatctt tccgcctgtc ggcagattaa
      721 tttccctggt tgcctgtccg atatcgggaa tgtcaacagc agtgactggc tccaccggaa
      781 caatgacgca agccataaaa ttgcttgttg gttttcccac tttacaaaga actgtgtatt
      841 gtgaagcata agaagcgtta gtaataaagc acttggaacc atttaaaatg taagaaccgt
      901 catccttctt gctgataaac gttgtcatca ttgaattatc cgaaccggca ttaggctccg
      961 ttaaacaaaa agaagccaaa agcggcttct cgacaaaggg ccgaagaaat aattcttttt
     1021 gctcatccgt cccatgttgg ccaattacga cattagccaa atcattgcac atggcggaag
     1081 tggaaattcc tgtatcacca taggaaagtt cttcgatgat caaagccgaa gaaatcacgt
     1141 caatctcaaa acctttgata gattccggaa tacacaaatt gataatgccc attccccaag
     1201 ctttgttaat aatcacccaa ggaaatttat gagctttttc catctccaaa atgtaaggcg
     1261 ttattccatt ttttaccaat ttcctcacag tgtctaaata catgagttga ttctcatcaa
     1321 gactcaaatc cataaaccaa acctttcgtc tctctatttt ttaggcataa atttataagg
     1381 aaactctctt tcttgagtca tttcttcaaa aaagggtact ccttattatt gctgctttat
     1441 gcggcaactt tttttttcgg gaaatttttc actcccgcac ttaccggcga atcaagccca
     1501 tatttcgttc gcaggaattt ttccatagtt gcgggatata agatatatgc tagcacatct
     1561 tcatcgttgc gggcaagatt accaataagt ttctttgccg cttcaatttc cggttccaga
     1621 taatcagccg gccttccgga aaccggctct tgtccgtgct tataattctt caatatcttt
     1681 ttagtcaatt ccgaatcaat cggcaccgga gttttaccgt acaatcccaa aaccagatct
     1741 ttaagctggt tagatactcg aacataacgg ccaaaaagaa catttaatac ggcctgcgct
     1801 ccaatgattt gcgataccgg tgtaaccagg ggcggatagc ccatgtcttt gcgtgtttga
     1861 actacttctt tgtaaatttc acccaatcga tctaaggcgt tcatttcttt gagttgacca
     1921 gtcaaattag aaatcatgcc gccgggaatt tgatgggcaa gaactccgtt atcgataatg
     1981 gagagcttat gattcgccag atggtgatta tatttcggag caatattttc caggtgttca
     2041 cccagcacca gcagtttgtg taaatccatt ccggtcgc
//
LOCUS       DASVIM010000186         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_840270, whole genome shotgun sequence.
ACCESSION   DASVIM010000186 DASVIM010000000
VERSION     DASVIM010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840270"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1352)
                     /gene="coaBC"
                     /locus_tag="VF305_03705"
     CDS             complement(141..1352)
                     /gene="coaBC"
                     /locus_tag="VF305_03705"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX7416270.1"
                     /translation="MLKGKKIVLGITGGIAAYKAAELTRALIKEGAQIKVIMTKCAAE
                     FITPLTLQTLSQNQVYTDMFLSADKYDMAHIALAEFADAFVIAPATGNIIGKIASGIA
                     DDLLSTTIMAAAKPTLICPAMNDKMLANPIVQENINKLKNCGYVVMDSAVGELACKTM
                     GVGRLTEIPEIVEEIERLLTPQNLAYEKILITAGPTEEPLDPVRFITNLSSGKMGYAL
                     ARVAHRHGAQVTLITGPTNLSLPSVENIIKVRTAQEMYKAVIENYKKATVIIKAAAVA
                     DYRPKVIASEKIKKDDKPRAIELERNQDIIAEIGKNKKNIVLVGFAMETKDLLANARE
                     KLKKKNMDLIVANSLREEGAGFQTDTNKITIIDREGDVQSLPVMTKIEAAEKILEKVA
                     GLLKKKGGSKK"
BASE COUNT          460 a          372 c          296 g          602 t
ORIGIN      
        1 tactgcggac aatgatttct tccattccaa tccaacagct aagctgtttt gattcaatat
       61 ttgtcctttg agtgccttaa caacaaagag caattctttc tgcaattgtt cgggtgttga
      121 ttgaaaaaaa gatttttcca ttacttttta ctgccgcctt tctttttcag caatcctgca
      181 accttttcca gaattttttc ggcggcttct attttggtca ttaccggtaa agattggaca
      241 tccccttccc ggtcaataat cgttatttta ttagtatcag tttgaaagcc agctccctct
      301 tcccttaagc tattggccac gattaaatcc atatttttct tttttaattt ttcccgggca
      361 ttagccagaa gatcttttgt ttccatcgca aaaccgacaa gtacaatatt ttttttgttc
      421 ttgccaattt cagctataat atcctgattt ctttcgagct caattgcgcg aggtttatcg
      481 tcttttttta ttttttccga agcaattact ttcggcctgt aatctgcaac tgccgctgcc
      541 ttgattatca ctgtcgcctt tttgtaattt tcaattacag ctttatacat ctcctgcgca
      601 gtgcgcacct tgataatatt ttccactgaa ggcaaagata aatttgtcgg gccggtaatt
      661 aaagtaactt gcgcaccgtg cctgtgagca actctggcca atgcataacc catttttcca
      721 gatgaaaggt tggtaataaa acgaaccgga tccagcggct cttctgtagg accggctgtg
      781 attaatatct tttcgtaagc tagattttgt ggagtcaaaa gtctttcaat ttcttctact
      841 atttctggta tttccgtcag acgtcctacc cccatggtct tacaagccaa ctcgcctact
      901 gcactatcca ttacaacata accacaattt ttcagtttat ttatattttc ctgaacaatg
      961 gggttagcca acatcttgtc gttcattgcc ggacaaatca acgtcggttt agcggcagcc
     1021 ataattgtgg tggaaagcaa atcgtcagcg attcctgagg caattttccc gattatattt
     1081 cccgttgccg gcgcgataac aaaagcatcg gcaaattccg ccaaggcaat gtgggccata
     1141 tcgtatttat ccgccgaaag aaacatatcc gtataaactt gattttgcga aagagtttgc
     1201 agagttaaag gtgtaataaa ttcggcagca cacttcgtca tgatgacttt tatctgcgca
     1261 ccttccttga tgagggcgcg agttaattcc gccgctttgt aggcggcaat cccacccgtt
     1321 attcccaaaa ctattttctt tcccttaagc ataatcttgt ctctatgttt gattttaaaa
     1381 taatataaca gcgttttatc acaagttcaa ggtataacta ccttaaaatt agatctatca
     1441 cgtaaagttt tttcttataa ttttacgttg aatattaaat agtttaccat tttacaagaa
     1501 aaaagctgta tcagcttaca gcgtcttaat gcgcaggatt cttcaaattt tttttatgca
     1561 ggtgaaaaag gaaaattacc ttgcaaaaga tgctaaaagc cattataagc cgcgcagctg
     1621 ttttatctca tagctttagg agcttattat gaataaatta agtttatata tcaaagtttc
     1681 catcgcaatt ttaatagtcg ccgtgctttg ctggctttat tttggcaatt
//
LOCUS       DASVIM010000187         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1098758, whole genome shotgun sequence.
ACCESSION   DASVIM010000187 DASVIM010000000
VERSION     DASVIM010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098758"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..144
                     /locus_tag="VF305_03710"
     CDS             67..144
                     /locus_tag="VF305_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416271.1"
                     /translation="MERLNKEFRRRTRPMEIMAGEAACY"
     gene            426..1550
                     /gene="dprA"
                     /locus_tag="VF305_03715"
     CDS             426..1550
                     /gene="dprA"
                     /locus_tag="VF305_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX7416272.1"
                     /translation="MNYDILKYWIALKSIAGIGNITFPALVDRFGSLPAIFAAAVSHL
                     RETPGISKETATAIAGFKDWDKIKHDLELLDKNEINIITYQDELYPVKLMNIYDRPAF
                     LYVRVNLNKDDINIAVVGSRQASTYGKYTTEKISRELALKGLTIVSGMARGLDSAAHR
                     GALTAHGRTIAVLGSGLDVIYPPENKKLYADIIQNGAVISEFPLGTPPRSANFPARNR
                     IISGMSYGVVIVEAGEKSGSLITARLALEQGREVFAVPGSIDAAGSRGTNKLIKQGAK
                     LIENVDDILEEILPQLENIKSLKLSSASNQKKNAVKKNEKINVIEKQIIDFISVEQIH
                     VDELISSTGLSSADILSAMTTLELKGIVQQHPGKFFSLKQ"
     gene            1612..>1933
                     /locus_tag="VF305_03720"
     CDS             1612..>1933
                     /locus_tag="VF305_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX7416273.1"
                     /translation="MANSLVVVESPTKVKTIKKFLGSDFNVVASMGHIKDLPKSVLGI
                     DLENNFEPTYNVIEAKKKTIDELKKAAKNAENIYLAPDPDREGEAIAWHISEVVNAKN
                     KNIYR"
BASE COUNT          615 a          381 c          407 g          530 t
ORIGIN      
        1 gattcttgct taacattttt tgactcccct gaggaagagt ggatatcatt aaggacgacc
       61 aatattattg aaagattgaa taaggagttt agaagaagaa ctcgaccgat ggagataatg
      121 gccggcgagg cggcttgtta ttaggatact ggcttttata gctataaaga tagaattaca
      181 ttggcggtca aactctgtga gaaaagtgcg taagaacctg tctttcttta aagaattggc
      241 ctatgacaat tttacacaaa aaaattgaca gtacacttat ccctctactt tcattgtgct
      301 aaaaggacta tcgcattcaa aatacttgac atacctgcta aacaagatgt aacttccgcc
      361 taatttttca tgaagaccgc atccacaggg cggagcttgt tggttgagct cctgatcaaa
      421 caattatgaa ttacgatatt ttaaaatatt ggattgcgct aaaatctatc gcgggcatcg
      481 gcaatattac ttttccggca ttagtggata ggtttggctc tctacccgct atttttgccg
      541 cagcggtttc acatttaaga gaaaccccgg gaatttccaa agaaaccgcc acagctattg
      601 ccggttttaa agattgggat aaaattaaac atgatcttga actcctggat aaaaatgaaa
      661 ttaatataat tacttatcaa gacgaacttt atcccgtaaa actcatgaat atttatgacc
      721 gtcccgcttt tctttatgtc cgggtcaatc tgaataaaga tgatataaat attgccgtag
      781 tcggttccag acaggccagc acttatggta aatacacaac ggaaaaaatc agccgcgaac
      841 ttgccctcaa aggtttaact atagtcagcg gcatggcccg gggccttgat tccgcagcac
      901 accgcggagc cctaactgca catggcagaa ctatcgccgt tctgggcagc ggactggacg
      961 ttatttatcc tccggaaaat aaaaaattat atgctgatat cattcaaaac ggtgcggtta
     1021 tttctgaatt tcctctgggc acgccgccgc gctcagccaa tttcccggcg cgtaatcgta
     1081 tcatcagcgg catgtcctat ggcgtcgtaa tagtcgaagc gggagaaaaa agtggttccc
     1141 tcattactgc gaggcttgct ttggaacagg ggcgagaagt ctttgccgtc cccggtagta
     1201 tcgatgccgc tggttctcgt ggaacaaata aattgattaa acagggagca aaattaattg
     1261 aaaatgtcga tgatatttta gaagaaattt tgccccagct cgaaaacata aagtcgttaa
     1321 aactatcatc agcttctaat cagaaaaaaa acgctgttaa gaaaaatgaa aaaataaatg
     1381 ttattgaaaa acagattatt gattttatct cggttgaaca aattcatgtt gatgaactta
     1441 tttcttcgac cggcttatcg tctgccgaca ttttaagcgc aatgacgaca ttggaattaa
     1501 aaggaatcgt tcagcagcat ccgggaaagt ttttttccct gaaacaataa aatgtcttta
     1561 caaattcaac tctgtcattt atagggtgcg gcgaaataaa ggagagcggt tatggcaaat
     1621 tctctggtag tggtggaatc acccacaaaa gttaaaacga ttaaaaaatt tctgggctct
     1681 gattttaatg ttgtcgcttc tatgggacat attaaagatc tgccgaaaag cgttttgggc
     1741 attgatctgg agaataattt cgagccgaca tataacgtaa ttgaagccaa gaagaaaaca
     1801 attgatgagt taaaaaaagc cgccaaaaac gcggaaaaca tttacctggc tcctgacccc
     1861 gaccgcgaag gagaagccat cgcctggcat atctcggaag tagtcaatgc gaagaataaa
     1921 aatatatatc gcg
//
LOCUS       DASVIM010000188         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_501081, whole genome shotgun sequence.
ACCESSION   DASVIM010000188 DASVIM010000000
VERSION     DASVIM010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501081"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1060
                     /locus_tag="VF305_03725"
     CDS             179..1060
                     /locus_tag="VF305_03725"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX7416274.1"
                     /translation="MNQGMQKTNFPKLNLITRGKVRDIYSIKDYLLIVATDRISAFDV
                     IMPNPIPGKGIILNRMSAFWFGQTEDIIGNHLISINPADFPAECASYRDALEGRSMLV
                     KKATPLPVECIVRGYLSGSGWKDYQHNKTVSGIKLPDGLKESSQIPEPIFTPTTKAPE
                     GEHDSAIARKEMEDIIGADIADKIIKVSLAIYERAAKIAKKAGIIIADTKMEFGLIGK
                     ELILIDELLTPDSSRFWPADDYQEGRPQKSFDKQFLRDYLLSINWNQKPPAPELPPEI
                     IENTRRKYEEALKRLVK"
     gene            1149..1547
                     /locus_tag="VF305_03730"
     CDS             1149..1547
                     /locus_tag="VF305_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416275.1"
                     /translation="MSSIKLQDYRLKQKILYIDKANPDVLQHYGDLFLEAGNISDALE
                     FYQKANNYSGMQKIKNIAFDSGDVMLFQQAAKALNLELKPIDWEGIGQKAIELKKYSF
                     AQHALEKANNEELVNSLKKIRQKEETEKTA"
     gene            1544..>2178
                     /locus_tag="VF305_03735"
     CDS             1544..>2178
                     /locus_tag="VF305_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7416276.1"
                     /translation="MTKKDYYEILGVIRTASEDEIKKNYRKIAMQCHPDKNPGDKKAE
                     ERFKEAAEAYEVLSDKQKRETYDHYGHEGLNNTGFRGFNGFDDIFSHFSDIFEDVFGY
                     GNVRSQSRSSARAGADLRYDLKISFLDAAFGLTTTIELEKLATCHKCHGTGAASGTAP
                     ETCRTCQGRGQVIQSSGFFTISSTCPHCNGHGKFITKPCGSCRGMGKEQVS"
BASE COUNT          709 a          472 c          449 g          548 t
ORIGIN      
        1 aacagattta cccggcgggt cctggcaggt aatgtataaa tccattcagg agaggctttt
       61 ctccctgccg gatgatacca aaataatgcc cggtcataat tacggccgta cgccaacttc
      121 cactataggt catgaaaaaa agaataatcc atcagtccga taaaggagaa aatcccttat
      181 gaatcaaggc atgcaaaaaa ctaatttccc caaattaaat cttattaccc gcggtaaagt
      241 gcgcgatatt tattccataa aagattattt gctcattgtc gctaccgatc gcatttcagc
      301 attcgatgtt atcatgccta atcccattcc cggtaaaggc ataattctaa accgcatgtc
      361 cgccttttgg ttcggacaga cggaagatat aattggcaat caccttatat cgattaatcc
      421 tgctgatttt cccgcagagt gcgcttccta ccgcgacgct ttggaaggca gaagcatgct
      481 ggttaaaaaa gctaccccgc taccggtcga gtgtattgtc cgcggttatt tgagcggttc
      541 aggctggaaa gattatcagc acaataaaac agtttccggc atcaaattgc ccgatggact
      601 aaaagaatca tcccagatac ccgaaccgat ttttacgccg accaccaagg cgccggaagg
      661 cgaacatgat tccgccattg cccgaaagga aatggaagat attatcggtg cagatattgc
      721 cgataaaatt atcaaagtca gcttagccat ttatgaacgc gctgcaaaaa tcgccaagaa
      781 agccggcatt atcattgcag acactaaaat ggaattcggt ctgatcggca aggaattaat
      841 cctgattgat gaattgctca ctcctgattc ttcccgtttc tggcctgccg atgattacca
      901 agaaggccgt ccgcaaaaaa gtttcgataa gcaattctta agagattatc ttctttccat
      961 taattggaat caaaagccgc ccgcaccgga actgccaccg gaaattattg aaaatacgag
     1021 gagaaagtac gaagaagcct taaaacgttt ggtaaaatga tttatttccg gccattgact
     1081 tagacaaaat cattattaaa ttatttagag tttatctata aagatatcac tcttttcagg
     1141 aggcactctt gagtagtata aaactacagg actaccgttt aaaacaaaaa atattgtaca
     1201 ttgacaaagc taatccggat gtgcttcagc attatggcga tttgttttta gaagcgggta
     1261 acatttccga cgcgctcgaa ttttatcaaa aggcaaataa ctatagcgga atgcagaaaa
     1321 ttaaaaatat cgcctttgac agtggcgatg tcatgctttt ccagcaagct gccaaagccc
     1381 tcaatttgga attaaaacct attgactggg aaggtatcgg acaaaaagct atcgagttaa
     1441 aaaaatattc atttgcccaa cacgccttgg agaaagcaaa taatgaagaa ctggtcaatt
     1501 cactcaagaa aataagacaa aaagaggaaa ccgaaaaaac cgcatgacaa aaaaggatta
     1561 ttatgaaatt ctaggcgtta ttagaactgc ctcagaagac gaaataaaaa agaattaccg
     1621 taaaattgca atgcaatgtc atcccgataa aaacccgggg gacaaaaaag cggaagaaag
     1681 attcaaagaa gcggctgaag cttatgaagt attaagtgac aaacaaaaaa gggaaacata
     1741 tgaccattat gggcatgaag gtttaaacaa cacggggttt agaggcttca atggcttcga
     1801 tgatatcttt tcccatttca gtgacatttt cgaggatgtt ttcggatatg gcaatgtaag
     1861 gagtcagtcg cgttcatctg cgcgtgccgg cgccgattta cgctatgatc taaaaatttc
     1921 tttcctcgat gccgcctttg ggcttaccac tacaattgaa ttggaaaaac tagctacctg
     1981 ccataaatgt cacggcaccg gagccgcttc gggaacagct cccgaaacat gtcgcacttg
     2041 tcaggggcgc ggccaggtaa tccaatccag cggttttttt acaatcagtt ccacctgccc
     2101 acattgcaac ggacatggca aatttatcac caaaccctgc ggctcttgcc ggggtatggg
     2161 caaagaacaa gtctctaa
//
LOCUS       DASVIM010000189         3509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_672405, whole genome shotgun sequence.
ACCESSION   DASVIM010000189 DASVIM010000000
VERSION     DASVIM010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672405"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VF305_03740"
     CDS             complement(<1..619)
                     /locus_tag="VF305_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7416277.1"
                     /translation="MKIAVLNGSPKGQISVTMQYVHFLQQKFPETEFKIFDIALKINK
                     LEKDNKAFQEIIAEISAADAVLWAFPLYFLLVCSQYKRFIELIFERKETSAFRGKHAA
                     ILTTSINFFDHTAHNYVNAICDDLQMNFVGSYSAEMNDLLKSQERQRLRTFGELFIKA
                     IKEGAPSIRNHKPLITREYVYEPAQPAPVETGGKKVLIVVDDMDKS"
     gene            complement(616..1284)
                     /locus_tag="VF305_03745"
     CDS             complement(616..1284)
                     /locus_tag="VF305_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX7416278.1"
                     /translation="MDLREPANAGKSRDSRFLKKILTSAEIEFVQTAENPDATLWSLW
                     ACKETAYKVIKKSCVDAAFLPRQWQIIFNKSESKCIDGKMIIPGKDGVYIRLFSNSHY
                     THCIGTDCLWALDKLICNVDVLPEEKDGENIDPSLFSRKCLGQNLAKFFCLNFQDIEI
                     TRVKMNGALQPPHVCVGGRKTDMDVSLSHDGQFVAYAFYPDCAIINKIKKTSVKIRNH
                     KKIL"
     gene            complement(1208..2032)
                     /locus_tag="VF305_03750"
     CDS             complement(1208..2032)
                     /locus_tag="VF305_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7416279.1"
                     /translation="MNKKSKYFLHLQNFLGRIAIIFIAPFYFFIVKILFYRIQNLKEI
                     RRQCEGEFARHKGSWIICANHLTMIDSFILGYAIFSLGRHITGYKKLPWNLPERSNFQ
                     SNLILATLCYLGKCIPIDRGGSREKIKKTLGKCVYLLRSGHNIMIFPEGRRSRTGRIN
                     KESFSYGVGRFVNDVEDCKVMCIYLRGNKQDNYSVIPAWGEKFSVQIEVFSPERVEES
                     GLRVQREYATQIIERLARMEERYFALRRERYRGFKRAGERREKQGFALSKENPHKC"
     gene            complement(2037..2285)
                     /locus_tag="VF305_03755"
     CDS             complement(2037..2285)
                     /locus_tag="VF305_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7416280.1"
                     /translation="MDEKKIFEGMVNILKAYTKDQALLAKATMDTHILNDLKVNSARL
                     VDVIIKCEDVYGISVDDDEADKIRTIGDAVKIIKQKLG"
     gene            complement(2310..>3509)
                     /locus_tag="VF305_03760"
     CDS             complement(2310..>3509)
                     /locus_tag="VF305_03760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX7416281.1"
                     /translation="REGRSGIRFIPLLEQLKFGCQIAGIPENFDAIRKNYFDREKLLS
                     INDNIGFASVSAVDAWRDAGFNLPDSNDDTVDWDTGVIAGSGIGGMDTIAQTIVPLVN
                     EGKVRRLGSRVVEQVMGSGTSARISGLIGAGNQVTSNSSACSTGNEAVIDALWRIRMG
                     LAKRMVAGGSEGATPYIWGGFDAMRVLCRKFNDNPTAGSRPMSATACGFVPGSGGAML
                     VLEELESALARGARIYAEIIGGYVNCGGQRMGGSMTAPNPEGVKKCIRGAVADAGINP
                     AEVDAINGHLTATYADPHEVRNWAEALERTPDNFPYINSTKSLIGHCLGAAGAIECVV
                     SVLQIYKGFIHPSINCEDVHPEIAEYAPKIPQKCLEFPKLRIVAKAGFGFGDVNSCII
                     FKKWDKN"
BASE COUNT         1011 a          843 c          640 g         1015 t
ORIGIN      
        1 aacttttatc catatcatca acaacgatta atactttttt accgcctgtt tccacaggag
       61 ccggttgggc aggctcataa acatactcac gcgtgatgag cggtttatga tttcttatcg
      121 aaggcgcgcc ctctttaatc gccttaataa ataattcgcc aaaagttctg agcctttgcc
      181 tctcttgaga ttttagcaga tcattcattt ctgccgaata ggaaccaaca aaattcattt
      241 gtaaatcatc gcaaatggca ttgacataat tgtgggcggt atggtcaaaa aagttgattg
      301 atgtcgtaag aatcgcggcg tgtttcccgc ggaaagctga ggtctccttt ctttcaaaaa
      361 ttaattcgat aaatctttta tactgagaac aaacaaggag aaaataaaga gggaatgccc
      421 agagaacggc atcggcagcg gatatttccg cgataatttc ctgaaaagct ttgttatctt
      481 tttccagttt attaattttt agcgcgatat cgaagatctt aaattcggtt tctggaaact
      541 tttgctgcag aaaatgaaca tattgcatcg ttacgctgat ctgacctttt ggcgaaccgt
      601 ttaaaactgc aatcttcata atattttttt atgatttcta attttaaccg atgttttttt
      661 aattttattg attatagcac aatcaggata aaaagcataa gcaacgaatt ggccatcatg
      721 acttaagctg acgtccatat cggttttcct gccgccgaca caaacgtggg gcggttgaag
      781 cgctccattc atttttactc tagtaatttc tatatcttgg aaattcaggc aaaagaattt
      841 agccaagttt tgtccaaggc atttacgcga aaataatgat ggatcaatat tttccccgtc
      901 tttttcttca ggcaaaacat caacattaca gattaattta tccaaagccc aaagacaatc
      961 ggtgccaata caatgggtat agtgggaatt ggaaaagaga cggatataaa ccccgtcttt
     1021 ccccggaata atcattttac catcaataca ttttgattcg gacttattga aaataatctg
     1081 ccactggcgc ggtaaaaaag cagcgtcaac gcaagatttt tttatcacct tatacgcagt
     1141 ttctttacaa gcccaaagcg accaaagcgt cgcatcagga ttttccgcag tttgtacaaa
     1201 ttcaatttca gcacttgtga ggattttctt tagaaagcgc gaatccctgc ttttccctgc
     1261 gttcgccggc tctcttaaat ccacgatatc gttcccgacg taaggcaaaa tatctctcct
     1321 ccattcgcgc gagccgttca attatttgcg tggcatattc tcgttgcact cgcagtccac
     1381 tttcctcgac acgttccggc gaaaacactt ctatttgcac tgaaaatttt tctccccaag
     1441 caggaataac actataatta tcctgcttat ttccccgcag ataaatacac atcactttgc
     1501 aatcttccac atcgttgacg aaacgtccca cgccataaga aaaactttct ttattaattc
     1561 ttccggtgcg ggaacgcctg ccttcaggaa aaatcatgat gttatggccg ctacgcagca
     1621 ggtaaacaca cttacccaaa gtcttcttta tcttttcccg ggatccgccg cgatcaatgg
     1681 ggatacattt gcccagatag cacaatgttg caagaataag attactttgg aaattgcttc
     1741 tttcgggtaa attccaaggc agcttcttgt aaccggttat atgccggcct aagctgaaaa
     1801 tggcataacc taaaataaaa gagtcaatca tcgtcaaatg attagcgcaa ataatccagg
     1861 atcccttgtg ccgggcaaat tcaccctcgc actggcgacg aatttctttc aaattctgta
     1921 ttcgataaaa taaaatttta acaataaaaa aataaaaagg cgcgataaat attatcgcaa
     1981 ttcgtcctaa aaaattctgt aaatgaagaa aatatttaga ctttttattc atttttttac
     2041 ccaagttttt gtttaattat tttcacggca tcgccaatag tgcgaatttt atccgcttca
     2101 tcgtcatcaa cgctgatacc gtaaacatct tcacacttaa tgatcacatc taccagacgg
     2161 gcggaattta ctttcaaatc atttaaaata tgcgtatcca ttgtcgcctt agccaaaagc
     2221 gcctggtcct ttgtataagc ttttaaaatg ttgaccattc cttcaaaaat ttttttctca
     2281 tccataaaac tattctcctt attgaaaaat taatttttat cccacttttt aaatatgata
     2341 cagctattga catcgccaaa accaaaaccg gctttggcga caattctcaa ctttggaaat
     2401 tctaagcatt tttgcggaat cttcggagca tattcggcaa tttccggatg tacatcctca
     2461 caattaatag aaggatgtat gaaaccttta taaatttgca gcacacttac tacgcattca
     2521 attgcgccgg ctgcacccaa acaatgccca atcaacgatt tcgtggagtt aatgtaaggg
     2581 aagttatcgg gagttcgttc cagtgcctct gcccaattcc ttacttcatg aggatcggca
     2641 tatgttgcag ttaaatggcc gttaatggca tcaacttcgg ccggattaat accggcatcg
     2701 gccactgcac cacgaataca cttcttcacc ccttcaggat taggtgcagt catggaacct
     2761 cccatccgct ggccgccaca attaacatag ccgccaatta tttctgcata aattctggcc
     2821 cctctggcca aggcactttc gagttcttcc agcaccaaca tggcaccgcc ggaaccggga
     2881 acgaagccgc aagcagtggc actcatcggg cgggagccgg ctgttggatt atcattaaat
     2941 ttacgacaaa gtacacgcat ggcatcaaag cctccccaga tgtaaggcgt ggcaccttcg
     3001 gaaccgcctg ctaccatgcg tttggccaat cccatcctta ttctccagag ggcatcgatt
     3061 acagcctcat taccggtgct gcacgctgaa gaattagacg tcacttgatt gccggcaccg
     3121 atcaaaccgg aaatgcgggc gctggtgccg ctgcccatca cctgttcgac aacacggctc
     3181 cccaaacgtc tcaccttgcc ttcgttaacc aggggaacaa ttgtctgggc aatcgtatcc
     3241 atgccaccga ttcctgagcc ggcaataact cctgtgtccc aatctaccgt atcgtcattg
     3301 ctatccggaa gattaaagcc cgcatccctc caagcatcca ccgccgagac cgaagcgaag
     3361 ccgatattat cattgatgga taaaagcttt tcacgatcaa aataattttt acgaatagcg
     3421 tcaaaatttt ccggtattcc cgcaatttga cacccaaatt tcaattgctc caaaagggga
     3481 atgaaacgaa taccggatcg gccttccct
//
LOCUS       DASVIM010000190         3929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_756158, whole genome shotgun sequence.
ACCESSION   DASVIM010000190 DASVIM010000000
VERSION     DASVIM010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756158"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VF305_03765"
     CDS             complement(<1..293)
                     /locus_tag="VF305_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416282.1"
                     /translation="MKKLFKSARLSLFFSLFLILLILVNALYASSGEGGSNEGNKWVE
                     FAWKTFDFVVLAGFLYWLLAAKVKEFFVGRRKNIKESLENTKQQKAEAEKKYR"
     gene            complement(290..724)
                     /locus_tag="VF305_03770"
     CDS             complement(290..724)
                     /locus_tag="VF305_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEX7416283.1"
                     /translation="MVSVIPDYTLLIQIVTFLILIYILNLLLYKPILAIIDRRKKQLE
                     ELENEIKLFKESVDKRVAEYEEKLKQAKANATELKKEIIKEGDNQAKSIVDAVRNEIP
                     LMTQEFQQKMDKEIQAARQILESQSRKLSLEIAEKVLGRSVQ"
     gene            complement(1049..1438)
                     /locus_tag="VF305_03775"
     CDS             complement(1049..1438)
                     /locus_tag="VF305_03775"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX7416284.1"
                     /translation="MWEKKQEVEDIKAFLGQGAEFIGKLIFNGSVRIDGNFQGEIYGQ
                     GSLVIGEGALVKANIAVKSIYIGGEVQGSIEAKEKIDIQSTGKFLGDVRTPVFIIEEG
                     AFFDGKSLMAEAVEKKMNTQQEDLSGS"
     gene            complement(1536..2651)
                     /gene="rodA"
                     /locus_tag="VF305_03780"
     CDS             complement(1536..2651)
                     /gene="rodA"
                     /locus_tag="VF305_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX7416285.1"
                     /translation="MIFLKFDRRIFYHFDWTLLALVLSICAIGILNIYSAGFSVGERQ
                     IPVYLKQTQWIALGIIFMTITFLIDYRTINQFAYIIYGISVAFLVLVFLHGSIMHGSQ
                     RWITLGIFSFQPSELIKLTIIIALARYFDDHKSPEPYKLKELFVPLLIVIIPFVLILR
                     QPDLGTALTLIIVFTSIVFFIGVNWKSVIITLSGGLILIPVGWFFLKNYQKERLITFL
                     NPENDPLGAGYHIIQSMIAVGSGGIFGKGFLNGSQTQLKFLPEQQTDFVFSVFAEEWG
                     FIGGLLLIIMFIALILWGLKIALHARDSLGTIIAFGIIALISWEVFINIGMVLGILPV
                     VGIPLPFLSYGGSAMVCLMTAIGLLMNISVRRFILQR"
     gene            complement(2692..>3929)
                     /gene="mrdA"
                     /locus_tag="VF305_03785"
     CDS             complement(2692..>3929)
                     /gene="mrdA"
                     /locus_tag="VF305_03785"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX7416286.1"
                     /translation="EVVEVNVYDKESKKLKRIDPISGYNATLTIDFDLQKAAWQAFEG
                     KAGAAVVLNPRDGSVLAMISSPSFDPNLFNSGISHDQWDKLQNNPLAPLSNKAISGQY
                     PPGSTYKLIMATAALEEGLITPQTKFSCNGFFKMGNRIFRCWKQHGHGQISLHQAIVE
                     SCDVYFYNVGKMLGVDKIAEYAKLLGLGAATGIDLPNEKNGLIPTKDWKLTWMKEPWQ
                     PGETISISIGQGFDLVTPLQLASAYSAFANGGTLWRPHLIKRIESTDGKIYKEFLPEK
                     KGELGLSKKTMEIINKALWGVVNEPGGTGHAARILNADVCGKTGTAQVVGLPGNEKAR
                     NAKKIGIFQRDHALFVCYAPLKNPEIVVAVILENAGHGGATAAPIARKILEAYFEGKK
                     NDKQPQVIAQSAPVNKVTR"
BASE COUNT         1168 a          857 c          683 g         1221 t
ORIGIN      
        1 tcccggtatt ttttctcagc ttcggctttt tgctgttttg tattttccag cgattcttta
       61 atgttttttc gccgcccgac aaaaaactct tttacttttg ccgccaagag ccaataaagg
      121 aaaccagcca gcacgacaaa atcaaatgtc ttccaggcga attcaaccca tttatttccc
      181 tcattgctgc caccctcgcc gctagaggca taaagagcgt ttactaaaat taaaagaatc
      241 aagaacaaag aaaaaaataa agacagccgt gctgatttga acaatttttt cattgaacgc
      301 ttctccccaa taccttttca gcaatctcta aagacaactt ccgggattgg ctctctaaaa
      361 tttgccgtgc cgcttggatt tctttatcca tcttttgctg aaattcctgc gtcatcagag
      421 ggatttcgtt acgcacggca tctacaatgc ttttagcctg attatcccct tctttgatta
      481 tttctttttt taattcggta gcatttgcct tggcttgttt gagtttttcc tcatattcgg
      541 caactctttt atctaccgat tctttaaaaa gcttgatttc attttccagt tcttcgagtt
      601 gttttttgcg gcggtcaatt atggcgagaa tcggtttata aagaagaaga ttaagaatat
      661 aaattaaaat taggaacgta actatttgaa tcaaaagcgt atagtcagga atgacactaa
      721 ccattgttta cctcatgctt ttttacgaaa aaagccgagt acaaaacatc ccttttgttt
      781 actcggcagt tttcattaat atgcataacc tcaaatgtat ggaacttttt gctcaagtgg
      841 tttttgaata tgtattaggt aaagcatagg gagcttttac ccttgatagc caaaaccgcg
      901 ggttattaat cataaccttg tttgcttgtc aagttttttc tgaccggcag tctaatttct
      961 cataaatata gattatagca ataataacgg gtaaaagcga ctagggagga tgtaaacgaa
     1021 aatcctctta caagtccgaa tttgttagtt acgatcctga taaatcttct tgctgagtat
     1081 tcattttctt ctccactgct tcggccatga gtgatttacc gtcaaaaaac gcgccttctt
     1141 ctataataaa taccggtgtg cggacatcgc ccaagaactt gcctgtggat tggatatcga
     1201 ttttttcttt tgcttcaata ctgccctgca cttcaccgcc tatgtaaata cttttaaccg
     1261 caatgtttgc tttaaccagc gcgccttcac caataactaa tgatccttgt ccgtaaattt
     1321 ctccttgaaa attgccatcg atgcggacgg atccgttaaa gataagttta ccaataaact
     1381 ctgctccctg gcctaaaaaa gccttaatat cttcgacttc ttgctttttt tcccacatat
     1441 cattctcctt atggtttttt gcaaattata ttgatcaatc tagctttgtc ggcaacgctt
     1501 tcagttgcca tatatttcta attggatatt aattactagc gctgcaaaat aaacctacgc
     1561 acactgatat tcattaataa gcctattgct gtcattaaac aaaccatcgc tgacccaccg
     1621 taacttaaaa aaggaagcgg aatgccaaca accggcaata tccctaaaac cattcctatg
     1681 ttgataaaaa cttcccacga aataagggca attattccaa acgctataat cgtgcctaat
     1741 gaatctctgg cgtgcaaggc aatttttagc ccccaaagaa taagtgctat aaacattata
     1801 atcaaaagta gcccgccaat aaaaccccat tcctccgcaa aaacggaaaa gacaaaatca
     1861 gtttgctgtt ccggcaaaaa ttttaattgg gtttgtgatc catttaaaaa acccttgcca
     1921 aaaattcctc ccgaacctac ggcaatcatt gattggataa tgtgataacc tgctcctaat
     1981 ggatcatttt cgggattaag aaatgtaatt aatctttctt tttgataatt ttttaaaaaa
     2041 aaccaaccaa cgggaattag tatcaagccg cccgataaag tgatgattac cgatttccaa
     2101 ttcactccaa taaaaaaaac aatggaagtg aaaacaataa ttaaggttag ggctgtaccc
     2161 aaatccggct gccttaagat gagtacaaag ggaataataa caattaataa aggcacaaat
     2221 agttctttta atttgtaagg ctcaggggat ttgtgatcat caaaataacg cgctaaggca
     2281 attattattg tcagttttat taattcggaa ggttgaaaag aaaaaatgcc cagagtaatc
     2341 catctttgtg aaccgtgcat tatagaacca tgaagaaata caagcactaa aaatgcaact
     2401 gaaataccgt aaattatgta agcaaactga ttgattgttc ggtaatcgat tagaaaagta
     2461 attgtcatga agattatgcc cagtgcgatc cactgagttt gtttcaggta aacgggaatt
     2521 tgtctttcgc caacgctaaa gcccgcagaa taaatattta aaatgccgat cgcacaaatg
     2581 gagaggacga gcgctagaag cgtccagtcg aaatgataga aaatgcgtcg atcaaatttt
     2641 aaaaaaatca tctgtcttcc ttagtcagga taatcaccaa tcattataaa atcagcgggt
     2701 tactttattt accggcgcac tttgcgctat aacttgtggc tgcttatcat ttttctttcc
     2761 ttcaaaatat gcttccaata ttttgcgggc gatcggagcg gcagtggcac cgccatggcc
     2821 ggcattttcc aaaatcactg cgacaactat ctccggattt tttagtggcg cataacaaac
     2881 aaataaagca tgatctcttt gaaaaatgcc gatttttttc gcgttacggg ctttttcatt
     2941 tccgggtaat ccgacaactt gtgcagtgcc cgttttaccg cagacgtcgg catttaatat
     3001 cctggcggca tgaccagtcc cccccggctc attaacgaca ccccacaaag ccttatttat
     3061 aatctccatt gtttttttac ttaaccccag ctctcccttt ttttccggca aaaactcttt
     3121 atatattttt ccgtccgttg actcgatgcg ctttatcaaa tgcggccgcc ataaagtacc
     3181 gccgttggca aaagcactat aggcactagc caattggagc ggtgtaacca aatcaaatcc
     3241 ttgaccgata gatatggata tagtttcacc aggttgccag ggttctttca tccatgtcaa
     3301 cttccaatct tttgtcggta tcagtccatt tttttcattg ggtaaatcaa tacccgtggc
     3361 tgcccctaac cccaaaagct ttgcatactc ggctatttta tccactccca acatttttcc
     3421 tacattataa aaatatacat cgcatgattc aacaatagcc tggtgaaggc tgatttgacc
     3481 gtgcccgtgt tgtttccagc atcgaaaaat tctattaccc atcttaaaaa aaccgttaca
     3541 agagaatttt gtttgaggag taataagtcc ttcttctaga gctgcagttg ccataatgag
     3601 cttgtaagtt gaaccgggag gatattgccc tgaaattgct ttattcgaca agggagccaa
     3661 cggattgttc tgcagtttat cccattggtc atgggaaatt ccactgttga acaagttggg
     3721 atcgaaagaa ggggaactaa tcatggccag tactgaacca tcgcgtggat tcaaaacaac
     3781 ggccgcacct gctttccctt caaatgcctg ccaagctgcc ttctgtaaat caaaatcgat
     3841 tgttaatgta gcattataac cggaaatggg atcgatccgt tttaattttt tactttcttt
     3901 gtcataaaca tttacctcga ccacttccg
//
LOCUS       DASVIM010000191         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993 k141_75753,
            whole genome shotgun sequence.
ACCESSION   DASVIM010000191 DASVIM010000000
VERSION     DASVIM010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75753"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..467)
                     /locus_tag="VF305_03790"
     CDS             complement(267..467)
                     /locus_tag="VF305_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7416287.1"
                     /translation="MSEGKVKWFNERKGFGFIEQEGGSDVFVHFSAIQASGFKTLNEG
                     QNVSFDVKQGKKGPEAENVKAV"
     gene            complement(729..974)
                     /locus_tag="VF305_03795"
     CDS             complement(729..974)
                     /locus_tag="VF305_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7416288.1"
                     /translation="MRSGSVRLNPGQDVGLHSTKQNEEMLVILDGQGEVELEGQALLK
                     ISDGQVTYVPPETRHNVRNTGSVPLKYVFIVSGAINS"
     gene            complement(949..1170)
                     /locus_tag="VF305_03800"
     CDS             complement(949..1170)
                     /locus_tag="VF305_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416289.1"
                     /translation="MIETIIEVKPVFFIVTDLTPKCPAHDGQEPQQAKAVADSRIPFS
                     MHASKQFEQIHRIPETTPDDYYAFRLSAA"
     gene            1221..2435
                     /gene="larC"
                     /locus_tag="VF305_03805"
     CDS             1221..2435
                     /gene="larC"
                     /locus_tag="VF305_03805"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX7416290.1"
                     /translation="MKILYYDCFAGISGDMNLGAMLDLGVDQKYLLKELGKLPLDSYK
                     IKVCKNKRGGITGTKVDVIVPPQKKTSSRHTLPKRTYRDITQMIKQSNLSANVKKISL
                     DIFTRLARAEGKIHGHKIDDVHFHEVGAIDSIVDIVGAAICLDYLKVDKVISSPIQVG
                     SGIINCSHGTLPVPAPATAEILQGIPIKTGLVPFEATTPTGAAIIASTAMSFTDRIDF
                     TPQKIGYGLGSKDSLIPNVLRLFLGEISTNAQKADDPETGEAVVIECNMDDMNPELYD
                     TLMDCLFSAGAHDVFFTPIIMKKSRPAVTVSVLCDVGKQKKIEEVFWLNSSTFGLRSY
                     KVAKSMLRREIVIVKTRHGKITVKNGYLNSRIIKSKPEYEDCKRLAKEKAVPIQDIYE
                     SIRLTKRTKNEH"
     gene            2425..>2925
                     /locus_tag="VF305_03810"
     CDS             2425..>2925
                     /locus_tag="VF305_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-amino-4-imidazole-
                     carboxylate carboxylase"
                     /protein_id="HEX7416291.1"
                     /translation="MNTDALKVLLENIRDGKIDVKAGISRLQNLSYIDLGCAKLDTHR
                     ELRVGYPEVIFCSGKTKEQIQTIIQAMMAGEANILATRASEENYAAVKKVCPAAVYHP
                     LARIITLERKKLPLPESYIAIVTAGTSDLPVAEEAAVTAEIFGNRVERIVDVGVAGVH
                     RLFDQLD"
BASE COUNT          873 a          632 c          632 g          788 t
ORIGIN      
        1 ttaccgtatg gaataattaa gaataaccgt ttacaaaagc aagaaggcag taactcaaat
       61 cttttatttt tgcaagttat attcagagga aagaaataaa atggaaactg aaatcgcgtc
      121 ggagtttgcc ccgtgcattc ggtgcggaac tgacgatagg aaggataatg tttgctggtt
      181 ggtttttatt taatatggtg agattataaa aaaagcactc ccgcgcaatt ttcatgtaca
      241 ggagtgcatc tttttttcag taacgcttat acagctttaa cgttttctgc ttccgggcct
      301 tttttgccct gcttaacatc gaagcttaca ttctgacctt cattcaacgt cttgaaaccg
      361 ctggcctgaa tagcactgaa atgaacgaaa acatcggaac caccttcttg ttcgatgaaa
      421 ccaaagcctt tgcgttcatt aaaccacttt actttacctt ctgacatagc ttgaatcctc
      481 ctttcttaaa tttccaagtc gaattggcac catgacagaa ctaaaaaacc gccaagacta
      541 tgaaagaaca tggcggctta cctttgtact tcaaaatgtc taaattcaac tctaacctgt
      601 atccttactt atcaaaatga tacatggata caaaactggt aaaaacctta cgcgtaatat
      661 caagtaaagt caagacatat ttaaatttat ttgtatggaa ataaagttaa attggggaat
      721 gttaaatatc aagaattaat tgccccagac acaatgaaga catatttcag gggtactgac
      781 cccgtattgc gcacattatg ccgggtctcc ggtggtacat aagtcacctg gccatctgat
      841 atttttagaa gggcctggcc ttcaagctcc acctctccct gaccgtccag aatcacaagc
      901 atttcttcat tttgtttggt cgaatgcagg ccaacatcct gaccgggatt aagccgcact
      961 gagcctgaac gcatagtaat cgtccggggt agtttcagga attctgtgta tttgttcaaa
     1021 ttgtttggaa gcatgcatgg aaaagggaat ccggctgtca gctaccgctt tagcttgttg
     1081 gggctcttga ccatcatgag ccggacattt cggcgtcaga tcggtcacta tgaagaaaac
     1141 gggcttgact tcaatgatag tttcgatcat attttgtaaa taacccgatt acttatgaat
     1201 gcagtgaagg gaatgatctg atgaaaatat tatactatga ttgttttgcc ggtataagcg
     1261 gtgatatgaa tctgggtgcg atgcttgatc tgggtgttga tcaaaaatat ttattgaaag
     1321 aactcggcaa actgcccctc gattcctata aaatcaaagt atgcaaaaat aaacgcgggg
     1381 gtattaccgg cacaaaagtt gatgtgattg ttccacctca gaaaaaaact tcttcccggc
     1441 atactctgcc aaaacgcaca taccgggata ttacacaaat gatcaagcaa agcaatttat
     1501 ccgccaatgt caaaaagatc agccttgata tttttacccg cctagctcgt gcggaaggaa
     1561 aaatccacgg acataaaatt gatgacgtgc actttcatga agtaggcgca atcgattcga
     1621 ttgtagacat agtcggagca gcaatctgcc tggactattt aaaggtcgac aaagttatct
     1681 cgtcacccat acaggtcggt tccggcataa ttaattgcag ccacggcaca ttgcctgttc
     1741 cggcgccagc cacggcggaa attcttcagg gtatccccat taaaaccggc cttgtgcctt
     1801 ttgaggccac aacgcccacg ggagccgcga ttattgcctc aacggcaatg tcttttacag
     1861 atagaattga ttttacgccg cagaaaatcg gttacggcct gggcagtaaa gattcactta
     1921 tccccaatgt cctgcgctta tttttgggcg aaatatccac taatgctcag aaagcggacg
     1981 atccggaaac aggcgaagct gtagtgatcg aatgcaatat ggatgacatg aatccggagc
     2041 tctatgacac gcttatggat tgcctttttt ccgccggagc gcatgacgta ttcttcacgc
     2101 cgattattat gaaaaaatcc cgtccggcag tgactgtttc tgtgctttgt gatgttggaa
     2161 aacaaaaaaa gattgaagaa gtcttctggc tgaattcatc caccttcggc ctgcgttcat
     2221 acaaagttgc caagtctatg ctgcggcggg aaatagtaat agtaaaaacc aggcatggca
     2281 aaattacagt aaagaatggc tacttaaata gccgcataat caagtccaag ccggaatatg
     2341 aagattgcaa gagactggca aaagaaaagg ctgttcccat ccaagatatt tatgaaagta
     2401 tccgcctgac taaaaggaca aaaaatgaac actgatgcat taaaggttct cttggaaaat
     2461 atccgcgacg gaaaaatcga tgtcaaagct ggcatctcca ggttgcagaa tctgtcttac
     2521 attgatttgg gttgtgccaa actcgatacg caccgcgagt tgcgagtcgg ttacccggag
     2581 gtcatcttct gctcgggcaa aaccaaagaa cagattcaaa ctatcatcca ggccatgatg
     2641 gccggagagg ccaacattct cgccacacgc gccagcgaag aaaattatgc tgcagtcaaa
     2701 aaagtttgtc ctgctgccgt gtatcatcca cttgcccgga taattacgct ggaaagaaaa
     2761 aaactgcctc tgcccgaaag ttatatagct attgtaacag caggaacatc tgatttgccc
     2821 gttgccgaag aagcagccgt tactgccgaa attttcggta atcgtgtgga aagaattgtg
     2881 gatgtcggtg tggctggcgt tcaccgttta tttgaccagt tggat
//
LOCUS       DASVIM010000192         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1102377, whole genome shotgun sequence.
ACCESSION   DASVIM010000192 DASVIM010000000
VERSION     DASVIM010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102377"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VF305_03815"
     CDS             complement(<1..1181)
                     /locus_tag="VF305_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX7416292.1"
                     /translation="MYQPGFAEFEKLAQKGNLIPVYREILADVETPVSVLMKLQNKDH
                     VYLLESVEGGEKWGRYSFLGTDAGIVFKVHGDKVIIEEKGRTTTREHKGNPLSGLRDL
                     LNRYKPVAIPGLPRFYGGAVGYLGYDMVRYFEKLPPSPPDDLNLDEAVFVVSDSMMIF
                     DNVRHTIKVVACAYTEETDSLEDSYAASCRKIEAMIETIAKAASHKTAAATGNEVNFE
                     SNMKPDQYRSVIEKAKEYIVAGDIIQVVLSQRFETDCRSNPIDLYRALRFVNPSPYLF
                     FLKLDDLTLIGSSPEVMVRKEDDTVELRPIAGTRRRGKTEQEDRALSDELLSDEKERA
                     EHVMLVDLGRNDLGRIAQTGSVQVNQYMVVEKYSHVMHLVSNVRAQLAKGKDAFDVLA
                     AT"
     gene            1580..3040
                     /locus_tag="VF305_03820"
     CDS             1580..3040
                     /locus_tag="VF305_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HEX7416293.1"
                     /translation="MDLLNFIFTVIKSIRFQDILDIAIVTLMIFALFTWFKTRASRFV
                     LIGILLLGGVYISARFFQLYLTTIVLQAFFAILLFVLVVIFQEDLRSFFEKLAMLGNI
                     GKRYRPLSELEKAAEVIAQTTGNLAKKHIGALIVLQGNDPLGRHINGGTSLDGLVSEP
                     LLESLFDPHSIGHDGAIIINGNRIRMFGCHLPLSIHTSKYGNIGLRHTAALGLAERSD
                     ALCIVVSEETGTISTAYQETLTKINNAATLHNILEEFYSRNAPARKSHNILPWFKENT
                     REKIIALAMACILWVAFGYQREIVRREFIIPIEYKNVPQNWRIDEPQVTEAKVILQGP
                     QQAYNLLDERSLKLSLDLSPLAGEKHEYALTKDVVNAPSNLSITEIRPAKIYVYAFKL
                     IARNLPVNINTQNVLHSNLSLQKVSVFPSHVRVLVDSRMGQEQIKIETEPVDLQKIFY
                     TTSLDTKIVLPAGVSFPEGKSPIARVTIRVKKKTAP"
     gene            3048..3311
                     /locus_tag="VF305_03825"
     CDS             3048..3311
                     /locus_tag="VF305_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416294.1"
                     /translation="MRAKFQKRSKKAGLSPGSLIHIGSRYAEESKITFIRYDETFYGE
                     KHLDSLADINNVNVFQEKEVALAMVNMLHQIKKCAQSIITLLK"
     gene            3324..>3569
                     /locus_tag="VF305_03830"
     CDS             3324..>3569
                     /locus_tag="VF305_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX7416295.1"
                     /translation="MQVFEASDIVEVAIRIEENGINFYKFAEQIAKQEEAKKLFAHLA
                     QAEVAHKKTFEGIFAKMEKYNPPESYAGEYSAYLHAYV"
BASE COUNT         1023 a          819 c          725 g         1002 t
ORIGIN      
        1 gtcgctgcca gaacatcaaa cgcgtctttt cctttggcta actgcgcgcg cacgttggac
       61 accagatgca tcacgtgtga gtatttttcc acaaccatat attgattcac ctgaacgctg
      121 ccggtttgag caatcctgcc cagatcgtta cgtcctaaat ccaccagcat cacatgctcc
      181 gcccgttctt tttcatcaga gagtaattca tcggaaagag cgcggtcttc ctgctctgtt
      241 tttcctcttc ttctagttcc ggcaatcgga cgcaattcca cagtatcatc ttctttgcgt
      301 accattactt ccggcgaaga accgatcagg gtaagatcat ccagcttgag aaaaaacaaa
      361 taaggagagg gattaacgaa acgcagcgcc cgatacagat cgatggggtt ggaacggcaa
      421 tcggtctcaa agcgttgcga gaggaccacc tgaatgatgt ctcccgcaac aatgtattct
      481 ttggctttct caatgaccga ccggtattga tcaggcttca tattggattc aaagttaact
      541 tcatttcccg tagcggctgc tgtcttatga gacgccgctt tcgcaatcgt ttcaatcatc
      601 gcctcaatct tccggcagga agcggcataa ctgtcttcca gtgaatctgt ctcctctgta
      661 taggcgcagg cgactacttt gatcgtatgg cgcacgttat caaaaatcat catggaatca
      721 ctgacaacaa aaacagcttc gtccagattc aggtcgtccg gcgggctggg cggaagtttt
      781 tcaaagtaac gcaccatatc atagcccaga taacctacag cgccgccgta aaaccggggc
      841 aggccgggaa tagcaacagg cttatagcga ttgagcaaat cacgaagccc actcagcgga
      901 ttgcccttat gctcgcgggt cgtggttctt cccttctcct cgataatgac tttatcgcca
      961 tggactttaa acacaatccc ggcatccgtc cctaaaaaag aataccggcc ccacttctcg
     1021 cctccttcca cactttccag aagataaacg tggtctttgt tctggagttt cattaaaacg
     1081 gaaaccggcg tttccacatc ggctagtatt tcccgataga ccggaattag attccctttt
     1141 tgagctaact tttcaaactc tgcaaaaccg ggctgataca taattttctc cttaacattc
     1201 acaaaaaaag gccgtgattt ggtcacggcc tttgaatgtg ctggaaataa aaaaagccgt
     1261 gaagatcccg gaggctctcc acggctttga ctttaattta aaagatcagc ggcggaaaat
     1321 cctccctgtg aggttccacc accaccaatt attaagagct aatattttaa ttatttgtgc
     1381 cataaatact ttccacttct tagttcgtgc ctttatagca atatgggaaa ctcctgtcaa
     1441 caattttttc agaaataaaa ttatgcatta atttacagga gaacatttaa agttagtttt
     1501 ttaatcttac ttgacactgg ccaagttctt tctatataag ttctattaag tttattaaag
     1561 agcaccctgg aaaaagttta tggatttatt aaattttatc ttcaccgtta ttaaaagcat
     1621 cagatttcag gatattttgg acatagcaat cgttaccctg atgatcttcg ctcttttcac
     1681 ctggttcaaa acaagggctt cccgcttcgt tcttatcggt atactccttc ttggcggcgt
     1741 ttatatttcc gcccggttct ttcaacttta tctcaccact atcgtcctgc aagcattttt
     1801 cgccatactg ctttttgttt tagtcgttat ctttcaggaa gacttgcgca gttttttcga
     1861 aaaactggcc atgctgggaa atatcggaaa aagataccgg ccgctgtccg agttggaaaa
     1921 agcagctgag gtaatcgcgc aaacgacagg taatttggct aaaaaacaca ttggcgcttt
     1981 aattgtcctg caaggtaatg atcctctggg aaggcatatc aacggcggaa caagtttaga
     2041 cgggcttgtt agtgaacctc tcttagaaag cctctttgat ccccattcca tcggccatga
     2101 cggagcaatc ataattaacg gtaatcgtat caggatgttc ggttgtcatc tacctctttc
     2161 catacatacc tcgaagtacg gcaatatagg attgcgccat actgctgcac ttggccttgc
     2221 ggagcgctca gatgcgcttt gcattgtggt ttctgaagaa actggtacaa tttcaacagc
     2281 ttatcaagaa acactcacta aaattaataa tgcggccact cttcacaata tattggaaga
     2341 attttatagc cgcaatgctc ctgctagaaa atcacacaac atcttgccgt ggtttaaaga
     2401 aaatacgcgg gaaaaaatta ttgccttagc tatggcttgc attttatggg ttgctttcgg
     2461 ctaccagagg gaaattgtgc gccgcgagtt catcattccc attgaatata aaaatgtccc
     2521 gcaaaattgg cggattgatg aaccgcaagt aaccgaagcc aaagtcatcc ttcagggacc
     2581 acaacaagcc tataatctac ttgatgaacg ttcgctgaaa ttgtctctgg atttatctcc
     2641 actggccggg gaaaaacatg aatatgcatt gaccaaggat gtggtcaatg cgccttcaaa
     2701 tttatccatt acggaaattc gccccgcaaa gatttacgtt tatgctttta agctcattgc
     2761 ccggaatttg cctgtaaata tcaacacaca aaacgtttta cacagtaatc tgtctttgca
     2821 aaaagtttca gtttttccct ctcacgtgcg tgttttagtc gattcccgta tgggacagga
     2881 acaaattaaa atcgaaacag aaccggttga cctgcaaaaa atattttata caacatccct
     2941 tgatacaaaa attgtactgc ccgccggagt ttcttttccg gaaggaaagt cacctattgc
     3001 ccgcgtgact atccgggtaa aaaagaaaac agctccgtaa ggtaaatatg cgcgctaaat
     3061 tccagaaaag atcgaaaaaa gccggacttt ctcccggcag cctaattcat attggcagca
     3121 ggtatgccga agaatcaaaa attactttca tccgatatga tgaaacgttt tatggggaaa
     3181 aacatttaga ctcgctggca gacattaata atgttaatgt atttcaagaa aaagaagtgg
     3241 ctctagcgat ggtaaatatg ttgcatcaga taaaaaaatg tgctcaatcc atcattacac
     3301 tattaaaata ggaggcgctt ggtatgcagg tttttgaagc aagtgatata gttgaggttg
     3361 ccatcaggat tgaagaaaac ggcattaact tttataaatt tgccgaacag atcgccaaac
     3421 aggaagaagc aaaaaaactt tttgcccatc tggcgcaggc agaagtcgct cataaaaaaa
     3481 cttttgaagg catctttgcg aagatggaaa aatacaatcc gccggagagc tatgcggggg
     3541 aatatagcgc ttatttgcat gcctacgtg
//
LOCUS       DASVIM010000193         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_586206, whole genome shotgun sequence.
ACCESSION   DASVIM010000193 DASVIM010000000
VERSION     DASVIM010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586206"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2283
                     /gene="gyrB"
                     /locus_tag="VF305_03835"
     CDS             <1..2283
                     /gene="gyrB"
                     /locus_tag="VF305_03835"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX7416296.1"
                     /translation="VVDNSIDEAAGGYCDTITVKIRVDNSIVVDDNGRGIPVDIHKTE
                     GVSAAEVVMTKLHAGGKFSNDSYKISGGLHGVGVSVVNALSQMCELEVRRDGNVYIQT
                     YTRGLPNGPLEMVGKTRGKGTKVTFLPDSEIFEDTEFSFDVISNRLRELAFLNSGVKI
                     TLTDERHDKKNEFFYKGGIISFVEYINRNKKVLHKNPIYIEGAKEDCSVEVALQYNET
                     YLENIFAFANSINTTEGGTHLIGLRSALTRVFNKYATSNKLIKDGKESLRGEDLREGL
                     ACVISVKLKNPQFEGQTKTKLGNSEVRGLVEGIVYEKIGSYLEENPSIAKQILNKCLD
                     AARAREAARRARELTRRKSALEVGSLPGKLADCQEKDPALSEIYLVEGDSAGGSAKQG
                     RDRKNQAILPLRGKVLNVEKARFDKMLQNEEIKIIVTALGTGIGTEEYDVSKLRYHKV
                     IIMTDADVDGSHIRTLLLTFFFRQMRELIERGYLFIAQPPLFKVVDKKHEFYIHNEEQ
                     MRNYILESGVEKINLVMDDGSRFPATGNKLLSLIKKIMRIEDFLDRFEKEQRDRHLLR
                     CLAGDPSLKDESFKSEKVLQPIAKRTLKAMKESVIDYVIEQDQDHGGYKIVFNLKKNG
                     QTLTTSMDRETFRSPQFSDIKTLLDQVSVVGEPPYRDVGEDKAEPVILESSKALVEHI
                     INIGKKGLTVQRYKGLGEMNPEQLWETTMNPEKRTLLQVSVDDAVLADEIFTTLMGDQ
                     VEPRRDFIYKYALDVSNLDI"
BASE COUNT          782 a          463 c          611 g          644 t
ORIGIN      
        1 gttgtcgata acagtattga tgaagcagcg ggcggctact gtgatacgat aacggtaaaa
       61 attagagtag ataatagtat tgttgtggat gataacggac ggggaattcc tgtcgatatt
      121 cataaaacag aaggtgtatc ggcggcagaa gttgttatga ccaagttgca tgccggcggt
      181 aaattttcca acgactccta taaaatttcg ggcgggttac atggggttgg tgtttcggtc
      241 gttaatgccc tttctcaaat gtgtgaattg gaagtgcggc gggatggcaa tgtttatatt
      301 cagacttaca cacgtggtct acctaatggt ccattggaaa tggtcggaaa aactcgcggc
      361 aaaggaacaa aagtaacgtt tttacctgac agcgaaattt tcgaagatac tgaatttagc
      421 tttgatgtta tttctaatcg cttacgagaa ttagcttttt taaattccgg tgtcaaaata
      481 actttaaccg acgagcgtca tgataagaaa aacgaattct tttataaagg cgggattatt
      541 tcttttgttg aatacattaa ccgcaataaa aaagttttac ataaaaatcc aatttatatt
      601 gagggtgcaa aggaagactg ttccgtagaa gttgccctgc aatataatga aacttatctg
      661 gaaaatattt ttgcttttgc caatagcatt aacacaactg aaggcggaac tcatttaatc
      721 ggtcttagat cggcgcttac tcgtgttttt aacaagtacg ccacaagcaa caaattgatt
      781 aaagacggca aagaatctct gcgcggcgaa gatttacggg aaggattggc ttgcgttatc
      841 agcgtcaaat taaagaatcc gcaatttgaa ggacaaacaa aaacaaagct gggcaacagc
      901 gaagtacgtg gtttggtgga aggcatcgtt tatgaaaaaa tcggcagtta tctggaagaa
      961 aacccttcta ttgccaagca aattctcaat aaatgtttgg acgcagcccg cgctcgagag
     1021 gcggcacgcc gtgcccgtga attgaccagg cgcaaaagcg cgctcgaagt aggctccttg
     1081 ccgggcaaat tagccgattg tcaggaaaag gatccggcgc ttagcgaaat ctatttggtg
     1141 gaaggtgatt ctgccggtgg ttcggccaag cagggacgcg atagaaagaa tcaggcgatt
     1201 ttacccctgc gcggtaaagt attgaatgtg gaaaaggcgc gtttcgacaa aatgctgcaa
     1261 aacgaagaaa ttaaaattat agttaccgct ttgggcacgg gcatcggtac cgaagaatat
     1321 gacgtaagca aactgcggta tcataaggta attatcatga ctgatgccga tgtcgatggc
     1381 tctcatattc gaacgttgct tttgactttt ttcttccgtc agatgcggga gctgatcgaa
     1441 agaggatatt tattcatcgc tcagccgccg ttgtttaaag tggtggataa aaagcatgaa
     1501 ttctatattc acaatgaaga acaaatgaga aattatattc tggaaagcgg agtggagaaa
     1561 attaatttgg tcatggatga tggcagccgc tttcccgcaa ccggcaataa attgttgagc
     1621 ttgatcaaaa agattatgcg gatagaggac tttttggacc gttttgaaaa ggagcagcgc
     1681 gacagacatc ttttacgttg cttggccggg gatccttccc tgaaagatga aagctttaaa
     1741 agcgaaaaag tattgcaacc gatcgcgaaa agaactctca aagccatgaa ggagagcgtg
     1801 atagactatg tcattgaaca ggaccaggac cacggaggct ataagattgt ttttaatctt
     1861 aagaaaaacg gacaaaccct gaccacttct atggatcgcg aaacatttcg ctcgccgcaa
     1921 ttttccgata taaaaacatt gctggatcag gtttccgtgg taggggaacc gccctatcgc
     1981 gatgtcggtg aggacaaggc cgaacctgtt attctggaaa gttctaaagc gttggtggaa
     2041 cacatcatta acatcggcaa gaaagggctg accgtccagc gctacaaagg attgggcgaa
     2101 atgaatcccg aacagctttg ggaaacgacc atgaatccgg aaaaaagaac tctactgcaa
     2161 gtcagtgtgg atgacgctgt tttggcagat gaaatattta cgacactgat gggagatcag
     2221 gtagaacctc gccgtgattt tatttataaa tatgctcttg atgtttccaa tttagacatt
     2281 taaacccgac gatcaaatac acaataaatg agccgttaaa taaccgctta tgtttagaga
     2341 ggatgaagca aaatggaaaa agatatccac cataagtcaa ctctggtgaa tatagaagat
     2401 gaaatgaaaa aatcgtacat ggattacgcc atgagcgtaa ttatcgggag agctatcccc
     2461 gatgtccgcg acggcttgaa accggtgcac cggcggattt
//
LOCUS       DASVIM010000194         2334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_929859, whole genome shotgun sequence.
ACCESSION   DASVIM010000194 DASVIM010000000
VERSION     DASVIM010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929859"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..935
                     /gene="sppA"
                     /locus_tag="VF305_03840"
     CDS             39..935
                     /gene="sppA"
                     /locus_tag="VF305_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX7416297.1"
                     /translation="MRKHPVIFGMLILLAMAVLFYIFFYKVGSHSSKSKRFSLNDKIG
                     VVSVDGPISDSIKITEQLDEFGKDDSIIAVILRVDSPGGGVAASQEIYDAVLELKKKK
                     KVVASMGSIAASGGLLVACAADKIVANPGTITGSISAIMQFANFEGLLKKIGLKSSVV
                     KSGKYKDIGSPLRDMTPEEKKIIQDLVDDIYNQFVDVVARERKLSRAKVIQIADGRVF
                     SGRKAKEYGLVDDLGDMGYAAKLASKLAGKDGKYDLVYPRKKRASIFDYLIESAANQL
                     RESLKEKIESWSGVSYLYYPAR"
     gene            951..1235
                     /locus_tag="VF305_03845"
     CDS             951..1235
                     /locus_tag="VF305_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7416298.1"
                     /translation="MTKNDLIKKLQEMLKYYSHKDLTYAVNIIFDSMIKAIRSEERIE
                     IRGFGNFTIRERKPRLGRNPKSGAEVQLAKRKVPFFKTGKELREMVNKKK"
     gene            1232..2008
                     /locus_tag="VF305_03850"
     CDS             1232..2008
                     /locus_tag="VF305_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7416299.1"
                     /translation="MSYKTIIIEKKNGYAVVKLNRPHEMNAISKEMRQELYKVFVDFE
                     GDAEVRAIVITGGEYVFSAGMDIKEMSNLSSEEGDEFLLSMMKYLKKIYSCKKPVIAA
                     VGGIALGGGFNLVTVCDLVVASESAIFCHPELKFGFNPFFYPLSQIVGITKAKEIVML
                     GEPIGANESLKIGLVNKVAPPEKFMEVAENMAEMLAKRSAKAVEELKNLCSIVPRMDK
                     MAALDIESSICALLFARDERKVQMNEVLSLEKSRKKKQKQ"
     gene            complement(2095..>2334)
                     /locus_tag="VF305_03855"
     CDS             complement(2095..>2334)
                     /locus_tag="VF305_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015506105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX7416300.1"
                     /translation="EVCTDSTCEWRLKNESFLNCTWVACNYGPFTLEEVGDMMGVTRE
                     RIRQIEAKALKKLQHKKRRDQLKDFAAPGNDWDNL"
BASE COUNT          765 a          388 c          524 g          657 t
ORIGIN      
        1 aagtaaaaat taacgaccag cagaaggatg aataaaacat gcgaaaacat ccggtaattt
       61 ttggaatgtt aatacttctg gcaatggcgg ttttgtttta tatttttttc tataaagtcg
      121 gttcgcattc cagcaaaagc aaaagatttt ctttaaatga caaaattgga gttgtcagtg
      181 tggatggtcc aatttctgat tccataaaaa taacggaaca attagacgag ttcggcaaag
      241 acgattctat cattgccgtt attttgcggg ttgattctcc cggcggaggg gtagccgctt
      301 ctcaagaaat ttacgatgcc gttttggaac ttaagaaaaa gaaaaaagtg gttgcatcta
      361 tgggttcaat cgccgcatca ggcggattgc ttgtggcctg cgcagcagat aagattgtag
      421 ctaaccccgg tactattacc ggcagtatct cggcaattat gcaatttgcc aattttgaag
      481 gattgctcaa aaaaatcggt ctaaaatctt cagtagttaa aagcggcaag tataaagata
      541 tcggttcgcc cctgagagat atgactcctg aagagaaaaa aatcatccaa gatttggttg
      601 atgacattta taaccagttt gttgatgtag ttgcaagaga aaggaagctt tctcgtgcga
      661 aagttataca aattgctgat ggtcgcgtgt tttccggcag gaaagcgaag gaatatgggc
      721 tagttgatga tcttggcgac atgggatatg cagcaaaact ggccagcaag ttagcgggaa
      781 aagacggaaa atatgatttg gtttatcccc gtaaaaagcg cgcatcgatc tttgattatt
      841 taatagaaag tgcggccaat caactgagag aatccttgaa ggaaaaaata gaaagttgga
      901 gtggcgtaag ttatttatat tatcctgcca ggtaaacggt aaaagttatt atgaccaaaa
      961 atgatttaat caaaaaatta caggaaatgt tgaaatatta ttcccataaa gatttaactt
     1021 atgctgtaaa tataatattt gattcgatga ttaaggcaat aaggagtgaa gagcgtatag
     1081 aaataagagg atttggcaat tttacaatta gagaaagaaa accgcgtctg ggacgaaatc
     1141 caaaatcagg cgccgaagtg caactggcaa aaagaaaagt tccttttttc aaaacaggga
     1201 aagaactgcg cgagatggta aataaaaaga aatgagctat aaaacaataa ttatcgaaaa
     1261 gaaaaatggc tacgccgttg ttaaattaaa ccgtccccat gagatgaacg cgatttccaa
     1321 agaaatgcgt caagaattgt ataaagtatt tgttgatttt gagggtgacg cagaagttag
     1381 agcaatagtt attaccggtg gagaatatgt attttcagcc ggcatggata taaaagaaat
     1441 gtccaacctg tccagtgagg aaggtgatga atttctttta tcaatgatga agtatttgaa
     1501 aaagatatat tcgtgcaaaa aaccggtgat tgcagctgta ggcggtattg ctttaggcgg
     1561 cggttttaat ttggttactg tttgcgatct tgtcgttgcc tccgaaagtg cgatattttg
     1621 ccatccggag ttaaaatttg gatttaaccc ttttttttac ccactgagtc agattgtcgg
     1681 tataaccaaa gcaaaagaaa tagtaatgct gggagaacct attggagcca atgaatcctt
     1741 aaaaataggt ttggtcaata aagtcgcgcc tccggaaaaa tttatggaag tggcggaaaa
     1801 tatggctgaa atgttagcga aacgttcggc caaagccgtc gaagaattaa aaaatctttg
     1861 ctcgattgtg ccgcgtatgg ataaaatggc cgcgctggat attgaatcgt cgatatgcgc
     1921 attgcttttt gcccgtgatg aacgcaaagt tcaaatgaac gaggtgttgt ctttggaaaa
     1981 atcacgtaag aaaaagcaaa aacaatgaga ctcaaatttc aaaagcgaag cgcattgaaa
     2041 tttgtaagtc gaattatccc gcgagtgtaa cgaagcggga tcaataacac caatctataa
     2101 attatcccag tcatttcccg gtgcggcaaa gtctttaagc tgatctctcc tctttttgtg
     2161 ttggagtttc tttaaggctt ttgcttcaat ttgccggatt ctttcacggg taactcccat
     2221 catgtcgcct acttcttcca atgtaaatgg cccataatta caggcgaccc atgtgcagtt
     2281 taaaaaagac tcgtttttta aacgccattc acaagtactg tcagtgcata cctc
//
LOCUS       DASVIM010000195         5180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1698183, whole genome shotgun sequence.
ACCESSION   DASVIM010000195 DASVIM010000000
VERSION     DASVIM010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698183"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VF305_03860"
     CDS             complement(<1..480)
                     /locus_tag="VF305_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX7416301.1"
                     /translation="MNLTAAGKTDQGLARSNNEDNYYVDVKSGLVMVADGMGGHSAGE
                     IASKLAINVISDYFQGENKFVRNYDQSYGPATNKLNSALCLANLAVFEAAQSSPQLKG
                     MGTTIAAALMIGNKLSIAHIGDSRVYLVRSDNIEQLTDDHSIVNEQVKRDVITKEEAV
                     "
     gene            766..1227
                     /locus_tag="VF305_03865"
     CDS             766..1227
                     /locus_tag="VF305_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7416302.1"
                     /translation="MCRADKEINDYQEIADIMKKAIVCRISLVDGDSPYIVPVNFAIH
                     DNHLYFHSAMEGKKIDILRKNNKICFEMDVNTEIIKGNEPCCWGMKYLSVIGFGQAYF
                     LEKSNEKKKALNVLMEKYAGVGSFSYPEAALKKVLVIDIRIEKISGKKSHI"
     gene            complement(1250..2515)
                     /locus_tag="VF305_03870"
     CDS             complement(1250..2515)
                     /locus_tag="VF305_03870"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX7416303.1"
                     /translation="MAEKLLPFTKKQIGKIIEKYPTPFHLYDEKAIRENARDFKKAFA
                     WNDGFKEFFAVKATPNPYLLKILHQEGFGVDCSSLAELVMAQKTGITGEEIMFSSNNT
                     PAAEFVKARELNATINLDDLTHIPYLEKHAGLPELICLRYNPGPLKKGNIIIGHPEEA
                     KYGFTREQLFEGYKICKAKGIKRFGIHTMVASNELDAAYHVETAEILFQLVADISRQL
                     KIRFEFVNLSGGVGIPYRPEQQRIDLAAMSRGIKEKYEKIIVAHGLAPIKIFRECGRY
                     ITGPYGYLISRVLHIKDTYKKFAGLDASMANLMRPAIYGAYHHITVIGKENMPHNFVY
                     DVTGSLCENNDKFAIDRPLPKLETEDIIVIHDTGAHGHSMGFNYNGKLRSAELLLREN
                     GTVVQIRRAETIDDYLATLDFDGLTNFAV"
     gene            complement(2543..4030)
                     /locus_tag="VF305_03875"
     CDS             complement(2543..4030)
                     /locus_tag="VF305_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HEX7416304.1"
                     /translation="MNILLIYPRYPVTFWSFKYILKYISKKAAFPPLGLLTVAAMLPK
                     KWNLQLIDLNIQKPKQKDWEWADYVMISAMLVQKDSVREILENCKQLKKKVVAGGPLF
                     TSKSEDYIHLVDHLILNEAECTLPQFLADLENGTPQKVYRSNSFPSLQNTPLPRWDLV
                     KTKHYATLMIQFSRGCPFNCEFCDVTTLFGHNPRTKTKGQFLQELQTVYDTGWRGALF
                     IVDDNFIGNKKAIKAMLPEVIEWMKARRYPFEMLTETSINIADDDELIQLMTQAGFQS
                     IFIGLETPNEESLKECSKNQNYKRDMTAAIKKLQAAGLQVFGGYIVGFDNDDESIFAQ
                     QIKFIQETGVVTAMVGLLNALPNTKLWHRLKKENRLADNCSGDNTDGSINFIPRMDKA
                     KLIAGYNKIVKTIYSPRHYYQRVNEFLKTYKPIPIIRKNKYSLRRLKALLKSVFYIGI
                     LGNGASQWYYWKLMLKLLLLYRKSFAEAMTLMVYGYHFRKIAKKL"
     gene            complement(4069..5070)
                     /locus_tag="VF305_03880"
     CDS             complement(4069..5070)
                     /locus_tag="VF305_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX7416305.1"
                     /translation="MLDSTGFVIFLVFIALVFDFLNGFHDSANSISTVVSTRVLSPKY
                     AVLWAAFFNFIAAFFIGTEVANTIGRGIIHLDIVDNLLILSALGGAIIWNIVTWYYGL
                     PSSSSHALIGGLIGAGIAKAGTSTLVWSGIIKTTAFIVLSPTIGLILGYFFMVLSLNL
                     YRNSNMARSDKLYRKLQLVSAAVYSLGHGMNDAQKTMGIIAMALYSKGLLGATFSIPF
                     WIVIACYSVIALGTMFGGWRIVKTMGTKITKLRPIGGFSAEAAAACAIIGASIAGIPV
                     STTHTITGAIVGVGATTRLTAVRWGVAGNIVWAWLLTIPVSALISAIIYLALKYFSG"
BASE COUNT         1545 a         1049 c         1063 g         1523 t
ORIGIN      
        1 taccgcttct tctttagtga ttacatcgcg tttaacttgt tcattaacaa ttgagtgatc
       61 atcggtcaac tgttcgatat tgtccgacct aactaaataa acgcggctgt cgccaatgtg
      121 ggcgatgctc aatttgttgc caatcattag cgctgcggca atagttgttc ccatcccctt
      181 cagttgggga gagctttgcg ccgcttcaaa aacagctaaa ttggccaaac acagcgcgga
      241 attaagctta tttgttgccg gaccgtaaga ttgatcataa ttgcgaacaa acttattttc
      301 cccttgaaaa taatcgctaa tgacattaat agctaattta ctggctattt ctcccgcaga
      361 atgaccgccc attccatcag ctaccattac aagtccagac ttaacatcta cataataatt
      421 atcttcgtta ttggaccgag ccagtccttg atcggtttta ccagcggcgg ttaaattcaa
      481 tttatgcatc ctcatcaaga cactcaactt ttttgaagta aattcaattt aaagatgcta
      541 ttaaagcatc catgaattct ttgcctgttt ggtatctcgc ttccgcgtct ttagctagta
      601 atttttccac aaatcgtcga ctttttaaat ttcttcatgt aaattccttt ttaaggttac
      661 acttaaaaag ctcaaacaat tttaaaacga tataaagtat ttttatcata actaatgaga
      721 atatagcaag atttcttgtg aatattttta tcataaggag gggaaatgtg cagagccgat
      781 aaagaaatta atgattatca ggaaatcgcc gatataatga agaaagccat tgtatgcaga
      841 atttctttgg ttgatggcga tagtccttat attgttccgg taaattttgc tatacatgat
      901 aatcatcttt attttcattc agctatggaa ggaaagaaaa tagatatcct ccggaagaat
      961 aataaaatat gtttcgaaat ggatgttaac acggagatta taaaaggtaa cgaaccttgt
     1021 tgttggggta tgaaatattt atctgtaatt ggctttggtc aggcttattt tctggagaag
     1081 tctaatgaaa aaaagaaggc tttaaatgtt cttatggaaa aatatgccgg cgtgggttct
     1141 ttttcttatc cagaggcagc tttgaaaaag gttcttgtta ttgacatcag aatcgaaaaa
     1201 atttccggca agaaatctca catatagtag agctttttta cgttatttat tacactgcaa
     1261 agttagttaa gccgtcaaag tctagagtag ccagatagtc atcaatggtt tcggcgcggc
     1321 ggatttgcac aactgtacca ttttctctga gcagtaattc tgctgaacgg agcttgccgt
     1381 tgtaattgaa gcccatggag tgaccatgcg ctcctgtatc atgaattaca ataatatcct
     1441 ctgtttccag tttgggcaga ggacggtcaa tggcaaattt atcgttgttt tcacagagtg
     1501 atcccgttac atcataaaca aaattatgcg gcatattttc ttttcctata acggtgatgt
     1561 gatgatacgc gccgtaaata gcgggacgca ttaagttcgc catgctggcg tcaaggccgg
     1621 caaacttctt ataagtatcc ttaatatgca ggacacggga aataagataa ccataaggac
     1681 ccgtaatata acggccgcat tccctaaaga ttttaatcgg cgcgaggcca tgagcaacaa
     1741 ttattttttc atatttttct ttgataccgc ggctcatcgc ggcaagatcg atacgctgtt
     1801 gttccgggcg gtagggaatt cccacacctc cgctcaaatt tacaaactca aaccgtattt
     1861 ttaattgccg ggaaatatcg gcaacaagtt gaaacaatat ttctgctgtt tctacatgat
     1921 acgcggcgtc caattcgttg gaagcaacca tcgtatgaat gccaaaacgt ttaattcctt
     1981 ttgccttgca tattttataa ccttcaaaga gctgctcacg agtaaagcca tatttagctt
     2041 cttccggatg gccgataata atattgccct tctttagcgg tccgggatta tagcgcaagc
     2101 aaatcagctc cggcagtccg gcatgttttt ctaaataagg aatgtgtgta agatcatcca
     2161 aattgatcgt tgcgtttaat tctctggctt tgacgaattc cgccgccggc gtgttattgg
     2221 aagaaaacat tatctcttcg ccggtaatac ccgttttttg agccatgact aattctgcaa
     2281 gcgagctgca gtcgactcca aagccctcct gatgaagaat ttttagcaaa taaggattag
     2341 gagttgcttt aacagcgaaa aattctttaa agccgtcgtt ccaggcaaaa gcttttttaa
     2401 aatcacgcgc gttttcacgg atagcctttt catcataaag atggaagggt gtcggatatt
     2461 tctcaattat ttttccaatt tgttttttcg taaagggcag taacttttcc gccataatcg
     2521 aatctacctg ttcgttaaaa aattacaatt ttttggctat tttacgaaaa tggtaaccat
     2581 aaaccattaa tgtcatagct tcggcgaaag acttgcgata aagaagcagc aatttcaaca
     2641 taagtttcca atagtaccac tgagatgcgc cgttgcctaa aatgccaata taaaaaacag
     2701 atttaagcaa tgctttcaga cgtcgcaggg aatatttgtt tttcctgatt attggtatcg
     2761 gcttatatgt tttgagaaat tcattgacac gttgataata atggcgaggg ctgtaaattg
     2821 tcttcacaat tttgttatag cccgcaatga gtttcgcttt atccatgcgg ggaataaaat
     2881 taatgctgcc atcggtattg tctcccgaac aattgtccgc gagacgattt tcctttttta
     2941 ggcggtgcca taattttgtg ttgggtaaag cgtttaataa gcccaccata gcagtaacga
     3001 cgcccgtttc ctggataaac ttaatctgct gcgcaaaaat actttcatca tcattatcga
     3061 agccaacaat atagccgcca aaaacttgta gtcccgcggc ctgcaatttt ttaatggcgg
     3121 cagtcatatc acgtttgtag ttttgatttt tggaacattc ctttaagctt tcttcattgg
     3181 gtgtttccag accgatgaat atgctttgga aaccggcttg agtcatgagt tgaatcagtt
     3241 cgtcgtcatc ggcgatgtta atggatgttt cggtgagcat ctcaaaagga tatctacggg
     3301 ctttcatcca ttcaatcacc tcgggcagca tggctttaat agcttttttg ttaccgatga
     3361 agttgtcatc gacaataaat agagctcccc gccagcctgt atcgtaaacg gtttgcaatt
     3421 cctgcaagaa ctgtccttta gtttttgtgc gcgggttatg gccgaataag gttgtaacat
     3481 cgcaaaactc acagttaaag ggacagccac gggaaaactg gatcatcaaa gtagcatagt
     3541 gttttgtttt taccagatcc cagcgcggta gcggtgtatt ttgcaatgaa gggaaactgt
     3601 tggagcggta aactttttgg ggagtgccgt tttccaaatc agccaaaaat tgaggcagtg
     3661 tgcattctgc ttcatttaaa atcaggtggt ccacaagatg aatatagtct tcggacttac
     3721 tggtaaacaa tggtccgccg gcaacaacct ttttcttcag ctgtttacaa ttttcgagga
     3781 tttccctgac cgaatctttt tgaaccagca ttgcgctgat catcacgtaa tctgcccatt
     3841 cccaatcctt ttgctttggc ttttgaatgt ttagatcaat caattgcagg ttccattttt
     3901 tcggcagcat agctgcgacg gtaagtaagc ccaaaggcgg gaaagcggcc ttcttggata
     3961 tgtacttaag gatatattta aaactccaga aagtaaccgg atagcgggga taaatcaata
     4021 aaatattcat attcaacctt taattaggga aggttcaaag aaaaaatgtt agccggaaaa
     4081 atattttaaa gcaagataaa taatggcgga aattagagcg gaaacaggaa tagtcaaaag
     4141 ccatgcccaa acaatatttc cggcaacgcc ccagcgcacg gcggtaagtc ttgtggtggc
     4201 gccgacgccg actatggcac cggtaatcgt atgggttgtg ctgacgggta ttccggcgat
     4261 agaggcgcca ataatagcac aggcagcggc agcttcagcg ctgaacccgc cgatgggacg
     4321 gagtttggtg attttagtac ccatagtttt aacgatgcgc cagccgccaa acattgttcc
     4381 caaggcaata acactgtaac aggcaataac aatccaaaaa ggaatagaaa aggtagcacc
     4441 caaaaggccc ttactataaa gggccatagc aataatcccc atggtttttt gtgcatcgtt
     4501 catgccgtgc cccaatgaat agactgctgc ggaaacgagc tgcaattttc tgtaaagttt
     4561 atcagagcga gccatattgg aattgcgata taaattgagc gaaagaacca taaaaaaata
     4621 gcctaaaatt aaaccaatag tgggtgaaag aacgatgaaa gcagttgttt tgataatgcc
     4681 ggaccagact agcgtactgg tacctgcttt ggcaataccg gctccgataa gaccgccaat
     4741 caaggcatga gaggaactgc tggggagtcc ataataccaa gtaacaatat tccaaatgat
     4801 ggccccacca agagcagaca aaattaacag gttatccacg atgtctaaat ggattatacc
     4861 tcggccgata gtattggcaa cctcagttcc aatgaagaaa gcggcgataa aattaaagaa
     4921 cgcagcccaa agcacagcat atttaggaga aagaacacgg gtggaaacaa cagtagatat
     4981 cgaatttgcc gaatcatgaa acccgttgag aaaatcaaaa accagcgcaa tgaaaacaag
     5041 aaatattaca aaacccgtcg aatcaagcat gttttaggac gatcccctcc acaatatttg
     5101 atacatcttc acacacatca attgcttctt cgatgcgttc aaaaatttct ttccatttta
     5161 aaagttcgat ggcatctttt
//
LOCUS       DASVIM010000196        12149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_163103, whole genome shotgun sequence.
ACCESSION   DASVIM010000196 DASVIM010000000
VERSION     DASVIM010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 12149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12149
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163103"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..643
                     /locus_tag="VF305_03885"
     CDS             107..643
                     /locus_tag="VF305_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416306.1"
                     /translation="MKHKVGKSSMSVILFLAGACMLALALSPSIASAKEFTYDKKPAF
                     SITYPSDWSDNPDNAAQYNLLFSAKGAAGVPVFNVQIRDIPAGATIADAGKLFKKLIL
                     DPEQNQDCKIISDKQTTLKDGTKANETLLKYKYQGWLPLQAVVIAVFKDNKWIRVDIH
                     QAASEDPELDVLHTLKFK"
     gene            complement(953..1027)
                     /locus_tag="VF305_03890"
     tRNA            complement(953..1027)
                     /locus_tag="VF305_03890"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(992..994),aa:Glu,seq:ctc)
     gene            complement(1029..1102)
                     /locus_tag="VF305_03895"
     tRNA            complement(1029..1102)
                     /locus_tag="VF305_03895"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1068..1070),aa:Gln,seq:ctg)
     gene            complement(1200..2600)
                     /gene="gltX"
                     /locus_tag="VF305_03900"
     CDS             complement(1200..2600)
                     /gene="gltX"
                     /locus_tag="VF305_03900"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX7416307.1"
                     /translation="MEKIRTRFAPSPTGFLHIGGARTALFNWLFTRHHGGEFVLRIED
                     TDQVRSTDESTKAILDAMTWLGLNWDEGPYFQAKRVDLHREMVQKLINEEKAYYCICK
                     PEELEAKRKQALAAGKKPKYDGTCRDKKLGKSVDSVVRFRGPNAGATIVEDLVKGNIS
                     FNNEELDDLIIERGDGYPTYNFAVVVDDALMNITHVIRGDDHVNNTQRQILLYQALGF
                     TVPKFAHVSMILGADKARLSKRHGATSAMAYKEMGFLPEALVNYLVRLGWAHGDQEIF
                     SLKELIKEFSLQAVGKSPAVFNPEKLLWLNAHYIKEAKPERIFEEMKPLWPESAGLAK
                     PELVRKVIADLQPRSKTLVELAAAADFYFVEDIQYESEAAQKFLTPDIGAYLKEIAAG
                     LPSLTNFSKEGIENFLQIFVQEREIKLKVIAQPLRVALTGKTVSPGIDEVMVTLGKNR
                     VIKRIQDAIAYIEAHN"
     gene            2767..3309
                     /locus_tag="VF305_03905"
     CDS             2767..3309
                     /locus_tag="VF305_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX7416308.1"
                     /translation="MSNSLKINVVLLPEEGQNFVFSEDGAWFKECFQNSKRFDFTLRK
                     VDVNCLITKTSGTVFIKGNLSATIDVCCSRCLEETNLPIGGDFAYTLVPAKAETEEDL
                     ELKAEELEISHYDGDFIDLTPIICEQIILQIPIKALCNEDCKGLCPQCGINLNASSCN
                     CHLDFVDNRMAVLKNFKVKN"
     gene            3329..3514
                     /gene="rpmF"
                     /locus_tag="VF305_03910"
     CDS             3329..3514
                     /gene="rpmF"
                     /locus_tag="VF305_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX7416309.1"
                     /translation="MPNPVKRHSKTRRNQRRANDFLKPPSLSLCPQCNEPKLPHRLCP
                     TCETYRGKDYKKPEKNA"
     gene            3535..4476
                     /gene="fabD"
                     /locus_tag="VF305_03915"
     CDS             3535..4476
                     /gene="fabD"
                     /locus_tag="VF305_03915"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX7416310.1"
                     /translation="MAKVALVFPGQGTQHVGMGSSLLAEFAVVRDVFDRASSVLGINM
                     RKLCLEGPQKVLDLTVNTQIAVLTLSIAIYSVFKEKTAIKPMVMAGHSLGEYTALYAA
                     DAIGFEDIFSVVQARAQYHQDAVPAGEGAMAAIIGLDDDIVESVCSEVRNSSAQVAVA
                     IHNTSQQIVVSGHTAAVEKAMLIAKQKGAQTVVKLPISAPCHCSLLQKAADMLSEKLA
                     QVEFHNPTTPVIPNCNPDVFYTKENARKLLLDQIVLPVKWRQTVEKMAAMGVETIVEI
                     GPKRTLCGLIKRINKNIQLMSVEDTGSLYKTVEILSA"
     gene            4570..4806
                     /gene="acpP"
                     /locus_tag="VF305_03920"
     CDS             4570..4806
                     /gene="acpP"
                     /locus_tag="VF305_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7416311.1"
                     /translation="MTLEEKVIKLVMEQLDVTREECVVDASFIDDLGADSLDLVELIM
                     EMEEVFGVEISDEELEHIRSIKNVIDFLKKKGIN"
     gene            4830..6071
                     /gene="fabF"
                     /locus_tag="VF305_03925"
     CDS             4830..6071
                     /gene="fabF"
                     /locus_tag="VF305_03925"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX7416312.1"
                     /translation="MKRRVVVTGLGALTPLGNSVRESWESAIAGKSGIGPITKFDSAP
                     FKSRIAGEIKNFDPLQFVEKQEVRRYDDFILYAIAASEMAMTDAKLTISPEISSRVGV
                     IIGSAIGGLATIESEIKVLFASGPRKISPFTVPAILANLGSGHVSIRFGAKGPINCAV
                     TACASGTSAIGDAYKTIAYGDADAMITGGVEAAITPLTVAGFCAMRALSARNDDPQKA
                     SRPFDKNRDGFVIGEGCGIIILEELSFARKRGARIYAELAGYGCTSDAFHMAAPPPGH
                     EGAARSMQVAIKDAGLNTTDIDYINAHGTSTPLNDLYETQAIKSIFGGHAQKLMVSST
                     KSMTGHMLGATGGVEAIFAVKAIQEGIIPPTINLDNPDPECDLDYVPNVARHKIINTA
                     MSNTFGFGGVNAVLVFKKYIV"
     gene            6104..7357
                     /gene="glyA"
                     /locus_tag="VF305_03930"
     CDS             6104..7357
                     /gene="glyA"
                     /locus_tag="VF305_03930"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX7416313.1"
                     /translation="MSFLEKVDPEINQAIDLETRRQAGNIELIAAENFVSEAVLEAQG
                     CIMTNKYAEGYPGNRYYGGCEFVDIAENLAIERCKKLFGADHVNVQPHSGVQANMAVY
                     FAAMQPGDTVLGMNLSHGGHLSHGTPANFSGIFYKIVSYGVSRDTETIDFSEVEKIAK
                     KHKPKMIIVGASAYPRTLDFQRFRKIADEVGAVIMADIAHIAGLVATGLHPTPVPLCE
                     YVTSTTHKTLRGPRGGFIMCKEAYAKTLNSRVFPGSQGGPLMHIIAAKAVAFKEALAP
                     EFKEYQRQIIKNAKAMADELKNQGFRLVSGGTDNHLMLVDLIAKGVTGIEAQEALDRA
                     AITVNKNGIPFDTKGPQVTSGIRIGTPAVTTRGMKENEMRQIASYIADVIKNINNEQK
                     IQAVAQKVKTLCAQFPLYQRRIKSL"
     gene            7528..8013
                     /locus_tag="VF305_03935"
     CDS             7528..8013
                     /locus_tag="VF305_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEX7416314.1"
                     /translation="MAKRKASWANLPEKINKRPDWDAYFLDIVEIVSKRSTCLRRAVG
                     AGLVRDRRILATGYNGAPSKLKHCLDIGCLREKLKVPSGERHELCRGLHAEQNAIIQA
                     ALHGVSAKESTLYCTNHPCVICAKMIINAGVVRIVIRDGYHDKLAAQMLKEAGISVKQ
                     L"
     gene            8039..8500
                     /gene="nrdR"
                     /locus_tag="VF305_03940"
     CDS             8039..8500
                     /gene="nrdR"
                     /locus_tag="VF305_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX7416315.1"
                     /translation="MKCPFCGHAENKVIDSRISKDGKAIRRRRECLDCQRRFTTYEFV
                     EDILPMVVKKDGRRESFDRMKIRNGVTKACEKRPISMESIEKIVENVEQACQEFQGEE
                     IPSTVIGEKVMNELKTLDGVAYVRFASVYRQFRDVGEFMSELKDLLSKGKK"
     gene            8505..9143
                     /locus_tag="VF305_03945"
     CDS             8505..9143
                     /locus_tag="VF305_03945"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX7416316.1"
                     /translation="MFTGIIEGLGKIKRLTMKGADAVLEIEAAIDLNDVSIGDSIAIN
                     GACLTVTARNQNIFNADVSAETLGKTTLKLLQAGAKVNLEKSLRVGGFIGGHFVLGHV
                     DATGRILSQTQKSGSLILAVEIDDNLSRYIVEKGSVAIDGVSLTVNKLEKGRFYVNII
                     PYTAENTTLVAKKVGDLVNIETDVLGKYVEKLLHSPRGIDKDFLAQHGFLNK"
     gene            9157..10359
                     /locus_tag="VF305_03950"
     CDS             9157..10359
                     /locus_tag="VF305_03950"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX7416317.1"
                     /translation="MAISTIIEAIDDIRSGKMIILVDDEDRENEGDLCIAAQFVSPEA
                     INFMARYGRGLICLTLSEGLADKLNLKPMVQDNKARFGTAFTISIEARNGVTTGISAA
                     DRATTIMAAVNPEAKPEDLVSPGHVFPIRARRGGVLVRTGQTEGSVDLCRLAGLTPAG
                     VICEIMKDDGAMARMPDLEVFAQEHNLKIVTIADLIDYRMQNESLIRRVAQATIPTCY
                     GGDFNIIVYENDVDQMQHIALVKGKFTEDDEVLVRVHSECLTGDVFASQRCDCGDQLH
                     CAMKMVEQEGKGVIVYMRQEGRGIGLANKIKAYALQEEGKDTVEANIALGFKPDLRDY
                     GIGAQILVDLGVRKMRLMTNNPKKIVGLEGYGIEITKRVSIEMDTNENNIEYMKTKQR
                     KMGHLLKF"
     gene            10575..11039
                     /gene="ribE"
                     /locus_tag="VF305_03955"
     CDS             10575..11039
                     /gene="ribE"
                     /locus_tag="VF305_03955"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX7416318.1"
                     /translation="MPKIIEGKIVAKGMKFGIVASRFNDFICSRLIDGAVDALMRAGA
                     EEKDIAIYKVPGSFELPLTAKKLAKSSRFDAVICLGAIIRGATPHFEYISAEVSKGIA
                     SVGLETEVPVVFGVLTTDTIEQAIERAGTKSGNKGADAAMTAIEMVDLFKKI"
     gene            11122..11577
                     /gene="nusB"
                     /locus_tag="VF305_03960"
     CDS             11122..11577
                     /gene="nusB"
                     /locus_tag="VF305_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX7416319.1"
                     /translation="MHGRRKAREIALQVLYSLNFVNIDVEKALDLFWGNFVAPKTAKE
                     FAATLVQGTWKHKEELDKLIAGCSDNWSLGRMSKVDISILRLAVFEFLYCDDIPPKVT
                     LNEAIDLGKTFGSENSGSFINGILDALNLKLNKENATNLLDKISRPSKT"
     gene            11588..12100
                     /locus_tag="VF305_03965"
     CDS             11588..12100
                     /locus_tag="VF305_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7416320.1"
                     /translation="MGIFADEKPPRGIGGFIDWRLNGLISREIKQGRISGEFMEKTII
                     PFPQRIGTEFLLLFGLGNLAAINYDRIYNAAYLIAGAVDGMSLPSFAFDLHGEGRCDL
                     ATSGIVEAMLTGFYDFLSADKKKISNMAACIVTSPSHLQEVSLGIKHFHANCKDKGSV
                     DVCALENSFA"
BASE COUNT         3450 a         2559 c         2929 g         3211 t
ORIGIN      
        1 tcttgtaatt ttatcttctt gacacacaag tgactccttc atatactcgg cccatcaaaa
       61 gtgaatagag tcgctgaact attataaaaa aaacaggagg taacttatga aacacaaagt
      121 aggcaaaagt agtatgtcgg taatattgtt tctggcaggg gcatgtatgt tggcattggc
      181 actttctcca tctatagctt cggcgaaaga gttcacctac gataagaagc cggctttctc
      241 cattacctat cccagcgatt ggagtgataa tcccgacaac gctgcccaat ataatttatt
      301 gtttagcgca aaaggtgctg ccggcgttcc cgtttttaat gtgcagattc gtgatattcc
      361 tgctggtgct actattgccg acgccgggaa actattcaag aagctcatcc tggacccaga
      421 acaaaaccaa gattgtaaaa tcatctcgga taagcaaaca actctgaagg acggaaccaa
      481 agccaatgaa acactactga aatataaata tcaaggctgg ctgcctttgc aggcggtcgt
      541 tattgcagtc tttaaggaca acaagtggat acgtgttgat attcaccagg cagcaagcga
      601 agacccggaa ttggatgttc tccacacatt gaaatttaag taatattcga ttaaaagcgt
      661 gaccctgtgg acttatagga gttcaacaag gtttatggga acgattttat agggagagga
      721 accgttttta gaaattcgtc tttccctaat tattgataat tttccggcgg ttggagctta
      781 atctccaacc gcttttttat ttacctggaa aatttagtta atagtcccct ttttctttta
      841 tatatatcca acagagataa gcacacaatt aagtgctgcc tcgcttctcg atcacgtatc
      901 gatatgtatt cgtcgctgtg cggggaatga tagggctaaa caaaaatgat tatggtagtc
      961 ccacgggggc ttgaaccccg gtttccggcg tgagaggcca gcgtcctaac cactagacga
     1021 tgggaccatg gctgggggac caggattcga acctgggtag cagggttcag agccctgagt
     1081 cctaccgcta gacgatcccc cagtaaagtt gagggtgagt atctattcaa accgattaca
     1141 aaagtcaagg cgaaattgaa gctgccacag gctatgggca atccggcagg caggccaatt
     1201 tagttgtgtg cttcaatata ggctatggcg tcttgaattc tcttgatcac gcgattttta
     1261 cctaaagtaa ccatgacctc atcaatgccg gggctgacgg ttttgccggt gagagcaacg
     1321 cgcagtggct gagcaataac cttcagtttg atttcccgct cttgaacaaa tatctgcaaa
     1381 aaattttcaa ttccttcttt gctgaaattt gtaagcgagg gaagtcccgc ggcaatctct
     1441 tttagatagg cgccaatgtc gggggtaaga aatttttgtg ccgcttccga ttcatactga
     1501 atatcttcaa caaaataaaa atcagcagcg gcagccagtt caactaaagt ttttgaccgc
     1561 ggctgcaaat cggcaataac ttttcttact aactccggct tggcaaggcc tgcggattcc
     1621 ggccacaaag gcttcatttc ttcaaagata cgctcaggtt tggcttcctt tatataatga
     1681 gcattgagcc agagtagctt ttcgggattg aaaaccgccg gcgatttgcc caccgcttgc
     1741 agagaaaact ctttaattaa ttcctttaga gaaaaaattt cctgatcccc atgcgcccag
     1801 ccgaggcgca ccagataatt aaccaatgct tccggcaaga aacccatttc cttataagcc
     1861 attgccgaag tcgctccgtg gcgtttactt aggcgcgctt tgtcggctcc cagaatcatg
     1921 gaaacatggg caaatttcgg aacggtaaaa cccagcgcct gataaagcaa aatctgacgc
     1981 tgtgtgttgt tgacatggtc atcgccgcgg attacatggg tgatgttcat cagcgcgtca
     2041 tcgacgacca cggcaaagtt gtaggtcgga tagccgtcgc cacgctcgat gatcaaatcg
     2101 tcaagctctt cgttattaaa gctaatgttt cctttaacca gatcttcgac aattgtcgca
     2161 cccgcatttg ggccgcgaaa gcgcacaaca ctgtccaccg atttacccaa ttttttatcg
     2221 cggcaagtgc cgtcgtattt aggcttttta cctgccgcca gagcctgttt tctttttgct
     2281 tccagttctt ccggcttgca gatgcaatag taggctttct cttcattgat taatttttgt
     2341 accatttccc ggtgcaaatc cacacgctta gcctgaaaat acggcccttc atcccaattt
     2401 aggcccagcc aggtcatggc atcgagaatt gcctttgtgg attcgtcggt agagcgtacc
     2461 tgatcggtgt cctcgatccg caagacaaat tcaccgccgt gatgacgggt gaaaagccag
     2521 ttaaaaagag ctgttcgcgc accgccgatg tgcaaaaaac cggttggcga aggagcaaag
     2581 cgagtcctaa ttttttccat tgttttattt cccttatttt ttatagcttt ataggattga
     2641 gatcaatctt ttgtcaagcc gcaattcctg aaaagcgtca aattatcctt gacaaggaaa
     2701 tgaatttata atttacttcc ccgcttaatg cggttggcga aattttgata aggcagcgaa
     2761 gttttaatgt ccaattcttt gaaaattaac gtagttcttc ttcccgagga agggcaaaac
     2821 tttgtttttt ccgaagacgg cgcctggttt aaggagtgtt tccaaaatag caaaagattt
     2881 gattttactc tgcgcaaggt ggacgtaaat tgtctgataa caaaaacttc cggcactgtt
     2941 tttattaaag gtaacctttc cgcgacaatt gatgtttgtt gcagccgctg tttggaagaa
     3001 acaaacctgc ccatcggtgg tgatttcgct tatacgttag ttcccgcaaa agccgaaacc
     3061 gaagaagatc tggagctcaa agcggaggaa ttggaaataa gtcattatga tggcgatttt
     3121 attgacctca cgcctattat ctgtgagcaa ataattttgc aaataccgat taaggcgctt
     3181 tgcaacgaag actgtaaggg tttgtgtccg caatgcggca ttaatttgaa cgcttcttcc
     3241 tgcaattgtc atttggattt tgtcgataat cgtatggctg tattaaaaaa ttttaaagtg
     3301 aaaaattaat cgtaaaaaag gataatttat gccaaatcca gtaaagagac attctaaaac
     3361 gaggcgaaat caaagaaggg cgaatgattt cttgaaaccg ccttcattgt ctttatgccc
     3421 acaatgcaat gagccgaaat tacctcatcg cctctgtccc acatgcgaaa cttatagagg
     3481 caaggattac aaaaaaccgg aaaagaatgc ctaatatcag aaacgagtat aacaatggct
     3541 aaagtggcgc ttgtttttcc cgggcaaggt acgcagcatg taggtatggg cagttcgtta
     3601 ttggcggaat ttgcagttgt aagagatgta ttcgaccgcg ctagttctgt cctgggcatt
     3661 aacatgcgta aactttgctt ggaaggacca caaaaagtcc tggacttaac ggtcaacaca
     3721 caaattgccg ttttgacgtt aagtatcgca atctatagcg tctttaagga aaaaacagcg
     3781 attaaaccca tggtgatggc cggccacagt ctcggcgaat atacagcttt atatgctgct
     3841 gatgctattg gttttgaaga tatcttctcc gtggttcaag cgcgtgccca atatcatcag
     3901 gacgctgttc ccgcaggcga aggcgccatg gctgccataa tcgggcttga tgatgatata
     3961 gttgaatccg tgtgctctga agtccgcaat tccagcgctc aagttgcggt ggcgattcac
     4021 aatacgtcgc agcaaattgt tgtttccggc catacggctg ctgtagaaaa agcaatgctg
     4081 atagcaaagc aaaaaggagc ccagacagtt gttaaactgc cgattagcgc tccctgccat
     4141 tgcagtttgc tgcaaaaagc ggcggatatg ctctccgaga aactggccca ggtcgaattc
     4201 cataacccga ctacgccggt tatccctaat tgtaatccgg atgttttcta tactaaagaa
     4261 aatgcacgca agcttctgtt ggatcaaatc gttttgccgg tgaaatggcg gcaaacagtg
     4321 gagaagatgg ccgcaatggg cgtggagaca attgttgaaa ttggtccgaa aagaacgctt
     4381 tgtggcttaa tcaaaaggat taataaaaat attcagttaa tgagcgtgga ggatacaggt
     4441 tccctatata aaaccgtgga gatactttcc gcatagaaaa aaataaggaa ttattttaca
     4501 aaaacaggta aaaggaatat catttcgctc aaatttttag ggcaataata gactggagga
     4561 ggattttgca tgacattaga agaaaaagtc attaaacttg tgatggaaca attggatgtc
     4621 acccgggaag agtgcgttgt ggatgcttct tttattgatg acttaggagc ggattcactg
     4681 gatcttgttg agctaatcat ggagatggaa gaagtttttg gcgtagagat atctgatgaa
     4741 gagttggaac acattcgttc aattaaaaac gtaattgatt ttcttaagaa aaaaggcatc
     4801 aattagttta tagtgcagga ttttagccaa tgaaaaggcg tgtcgttgta acaggcctcg
     4861 gggcattgac ccctttggga aattccgttc gggaatcttg ggaaagtgcc attgcgggaa
     4921 aatccggtat cggcccgatt accaagttcg atagcgctcc ttttaaatcc agaattgccg
     4981 gcgagataaa gaatttcgat ccgctgcaat ttgtggagaa gcaggaagtt cgccgctacg
     5041 acgattttat tttatatgcc atagccgctt cagaaatggc tatgaccgac gcgaaactga
     5101 caatcagtcc ggaaatttcc agccgcgttg gtgtaataat tggctctgct attggcggct
     5161 tggccacaat agaaagcgaa ataaaagtac tgtttgcctc cggccctcgc aagatttctc
     5221 cttttaccgt tccggcaatt ctggccaatt taggctccgg tcacgtctcc attcgctttg
     5281 gagccaaagg ccccataaat tgcgccgtta ccgcctgcgc ttccggaaca tcggcgatcg
     5341 gcgacgctta taaaaccatt gcctatggag atgccgatgc catgattacc ggcggtgtcg
     5401 aagcggcaat cactcctctt actgtggccg gtttttgcgc catgcgggcg ctatcggcga
     5461 gaaacgatga cccgcaaaaa gccagccgtc catttgataa aaatcgtgat ggttttgtta
     5521 ttggagaggg ctgcggtata attattttgg aagaattatc gtttgcccgg aaaagaggcg
     5581 cgcgtattta cgcggaactc gccgggtatg gatgcacatc cgacgctttt catatggccg
     5641 ctccgcctcc cggacacgaa ggtgccgccc gcagtatgca ggtcgcaata aaagatgccg
     5701 gcttgaatac gactgatatt gattacatca acgcccacgg cacatcaacg ccgctaaatg
     5761 atttatatga aacccaagcc atcaaatcca tttttggggg gcacgcccaa aagttgatgg
     5821 tcagctccac aaagtccatg accggccaca tgctcggtgc gacaggggga gtggaagcta
     5881 ttttcgccgt taaagctata caggaaggca ttattccgcc gacaattaat ctggataatc
     5941 ctgatcccga atgcgatctt gattacgtac ccaatgtggc gcggcataaa ataataaaca
     6001 cagccatgtc caatactttt ggttttggcg gagtcaacgc tgttttagta tttaaaaaat
     6061 atatcgtctg agaagataaa gtgcgaagga ggaatctgtt ttcatgtctt ttttggaaaa
     6121 agttgatccg gaaattaacc aggccattga cttagaaaca cgccggcaag cgggaaatat
     6181 tgaactgatc gctgcagaga attttgtcag tgaagcggta ttagaggcgc agggctgtat
     6241 aatgaccaac aagtacgctg agggttatcc cggtaatcgt tattatggcg gctgcgaatt
     6301 tgtcgatatt gcagaaaatt tagcgatcga acgctgcaaa aagctttttg gagctgatca
     6361 tgtcaacgtc cagccccatt ccggcgtaca ggcgaatatg gccgtatatt tcgcggctat
     6421 gcaaccgggt gacactgttc tgggaatgaa cttgtcgcat ggcgggcatt tgtctcatgg
     6481 aaccccggct aatttttccg gcatatttta taaaattgtc tcttacggcg taagcagaga
     6541 tacggaaaca atcgattttt ccgaagtgga aaaaatagcc aagaaacata aacccaaaat
     6601 gattattgtc ggcgccagcg cttatccgcg gacgcttgac tttcagagat tccgcaaaat
     6661 tgccgatgaa gtcggcgcgg taattatggc cgacatcgcc catattgcag gacttgttgc
     6721 cacaggattg catccaacgc ctgtacctct ttgcgaatac gtaacttcca cgacacataa
     6781 aaccttgcgt ggaccgcgcg gcggatttat tatgtgcaaa gaagcttatg ccaaaacctt
     6841 aaacagcagg gttttcccgg gctctcaggg cggaccgctg atgcatatca tcgcggccaa
     6901 ggctgttgct tttaaggaag cgcttgcgcc tgaatttaaa gaataccagc ggcaaattat
     6961 taaaaatgcc aaagcgatgg cggacgaatt aaagaatcag ggtttccgtc ttgtttccgg
     7021 cgggacagac aatcatctga tgctcgttga tttgattgcc aagggtgtta ccggcataga
     7081 agcccaggaa gctcttgacc gggcggctat taccgtcaat aaaaacggca ttcctttcga
     7141 tacgaaagga ccgcaagtaa ccagcggaat cagaatcggc acgcctgctg tcaccacaag
     7201 aggcatgaag gaaaacgaga tgcgccaaat tgcttcctat attgccgatg taattaaaaa
     7261 tatcaacaat gagcagaaaa ttcaggccgt agcccaaaag gttaaaactc tttgcgccca
     7321 gttccctctt tatcaacgga gaattaaaag tctgtaaaaa ggcttatacc aattctaaat
     7381 caaagacgat atcgaggcgg caaggaggaa gcgacgaagc gtattacaca tacgttgagg
     7441 aagccgacga cgctgccaac aaagatagcg ctttgattta gaattggtat aagacgaaaa
     7501 attttagctt cgcgaaagca tgtaactatg gccaaaagga aagcatcttg ggcaaacttg
     7561 ccggagaaaa ttaataaaag gcccgattgg gatgcttact ttctggatat tgtagaaatt
     7621 gtatccaaac gcagtacgtg tctgcgccgg gctgtggggg ctggattggt acgtgatcgc
     7681 cggatactgg ctaccgggta taacggcgct ccttctaaac ttaaacattg cttggatatt
     7741 ggctgtctgc gcgaaaagct caaggtgcct tccggtgagc gccacgagtt atgccgcggc
     7801 ctgcacgcgg aacaaaatgc cattattcaa gcggcattgc atggagtaag cgccaaagaa
     7861 tcgacgctgt attgtaccaa tcatccctgc gttatttgcg ctaagatgat catcaatgcc
     7921 ggcgttgtgc gcatagttat ccgcgatggt tatcacgaca agttggctgc acagatgctc
     7981 aaggaagcag gaattagcgt aaaacaactc taagttaaga aattatggtg acacaagcat
     8041 gaagtgtccg ttttgcggcc atgcggaaaa caaagttatt gattcgcgaa taagcaaaga
     8101 cggcaaagcc atccgccgcc gccgggaatg cttggattgc caaaggcgct ttaccactta
     8161 tgagtttgtc gaagatatcc tgccaatggt cgtaaaaaag gacggccgcc gcgagtcgtt
     8221 tgaccgtatg aaaatccgca atggagtgac gaaggcttgt gaaaagcgtc cgattagcat
     8281 ggagtcgata gaaaaaatcg tggaaaatgt ggagcaggcc tgccaggaat ttcaaggcga
     8341 agaaattcct tcgaccgtta tcggagaaaa agttatgaat gaactcaaga ctctcgatgg
     8401 tgttgcttat gtgcgctttg cctcggtcta ccggcaattt cgcgatgttg gggaattcat
     8461 gtccgaacta aaagatttat taagtaaggg taaaaaataa ttttatgttt accggaatta
     8521 tagaaggatt gggcaaaatt aagcgcctga caatgaaagg cgccgatgcc gttttagaaa
     8581 ttgaagccgc aatcgactta aatgatgtgt ccataggcga tagcattgcg ataaacggag
     8641 cttgtcttac cgtcaccgcc agaaatcaaa atatttttaa cgctgatgtt tccgccgaaa
     8701 cgctcggtaa aacaacgctc aaacttttac aggcaggcgc taaagtcaat ttggaaaaat
     8761 cattgcgggt aggcggtttc ataggcggcc attttgtttt aggacatgtt gacgccaccg
     8821 gcagaattct ttcccaaacg caaaagtccg gctcgcttat tttggctgtg gaaatagatg
     8881 ataatctttc gcgctatatt gtagaaaagg gttcggtggc catcgacggc gtaagcctta
     8941 ctgtaaacaa acttgaaaaa ggccggtttt atgttaatat aatcccttac acagcagaaa
     9001 atacaacttt agtggcaaaa aaagtagggg atttagtcaa cattgaaacg gatgttttgg
     9061 gcaagtatgt ggaaaagtta ttgcattctc cccgaggaat agataaggat ttcctggcgc
     9121 agcacggttt tttaaataag taaggataaa taaacaatgg caataagcac tattatagaa
     9181 gcaatagatg atattcgcag cgggaaaatg ataattctcg ttgatgacga agatcgggaa
     9241 aatgaaggcg atctttgtat agcggcacag tttgtttcgc cggaagcgat taatttcatg
     9301 gcccgctatg gccgtggttt gatatgtttg actcttagcg aaggacttgc cgacaaactt
     9361 aacctaaaac caatggttca ggataataag gcgcgttttg gaacggcgtt tacaatttct
     9421 atcgaagcgc gaaacggcgt caccaccggc atatccgccg ccgacagggc aacaacaatt
     9481 atggcggccg ttaatcccga ggcgaagccg gaagatttag tcagcccggg tcacgtattt
     9541 ccgatccgtg cccgccgggg cggtgtttta gtgcgcacgg gacagactga agggtcggtg
     9601 gatttatgcc gcctggccgg tttgacgccg gccggtgtca tttgtgaaat catgaaagat
     9661 gatggcgcaa tggcgcggat gccggatttg gaagttttcg cccaggagca caacctaaag
     9721 atcgtcacta ttgccgatct tattgattac cggatgcaaa atgaatcgct tatcagaagg
     9781 gtggcccagg caacaattcc tacctgctac ggtggcgatt tcaatattat cgtttacgaa
     9841 aatgacgttg accaaatgca gcacattgct ttggttaaag gcaagtttac agaggatgat
     9901 gaagtgctgg tgcgcgtgca ttcggaatgt ttaaccggcg atgtttttgc ctctcaaaga
     9961 tgcgactgcg gcgatcagct ccactgcgcg atgaaaatgg tagagcaaga gggaaaaggc
    10021 gttatcgttt atatgcggca ggaaggacgg ggaatcggcc tggctaacaa aatcaaggct
    10081 tacgctttgc aagaagaagg caaagatacg gttgaagcca atatcgcgct gggttttaaa
    10141 ccggacttaa gggattacgg tatcggggcg cagattcttg ttgatctggg agtgcgcaaa
    10201 atgcgattga tgaccaataa ccctaagaaa attgtcggac tggaaggtta cggaatcgag
    10261 atcactaaac gagtttccat tgaaatggac acgaatgaaa acaacataga gtatatgaag
    10321 acaaaacaaa ggaagatggg gcatcttttg aaattctagg aagcacagaa agcatttgtc
    10381 tttttgcgca agtaaaagaa tcccgctggg cttaagcttg cgggataaat tcaacttaca
    10441 agtttcagcg tgtgaccttt aggttactcg cttcgtttct gaaacttgag gttcattaat
    10501 ttgactaaaa tttttgcttc gcttagcttg caaaaattaa gtctcattaa cacttacaag
    10561 gatggtaata aatcatgccg aaaataattg aaggaaaaat tgtcgccaag ggaatgaagt
    10621 ttggcattgt ggccagccgt tttaacgatt ttatttgcag cagattaatc gacggagccg
    10681 tcgacgcatt gatgagagct ggcgcggagg aaaaagatat tgctatctat aaagtgcccg
    10741 gttcttttga attaccgctc acggcgaaaa aattggcaaa aagctcccgc tttgacgcgg
    10801 taatttgtct gggggcaata attcgcggcg cgaccccgca tttcgagtat atcagcgccg
    10861 aagtatccaa aggcattgcc agcgtcggtc tcgaaacaga agttcctgtg gttttcggcg
    10921 tattgacaac ggacaccatc gaacaagcaa tcgaacgcgc gggaacaaag tcgggaaata
    10981 aaggcgccga tgcggcgatg acggcaatag aaatggttga tttattcaaa aagatttaat
    11041 accaaattgg cattatttta ttgcgaagag atcaaaaacc aacttggtaa tctaaattag
    11101 ttctttgagg ttcgagttta aatgcacggg cggagaaagg cacgggaaat tgccttacag
    11161 gttctttaca gccttaattt tgttaatatt gatgtggaaa aggctttaga tcttttctgg
    11221 ggtaattttg ttgctccaaa aacagcgaaa gaatttgccg cgactttggt acaaggaaca
    11281 tggaaacaca aagaggaact tgataagctt attgccggtt gttcggataa ctggtcgctg
    11341 ggacggatgt ccaaagtcga cataagcatt ttacgtttgg ctgtatttga gttcctctac
    11401 tgtgatgata ttccgccgaa agttaccttg aatgaagcca ttgacctggg aaagactttt
    11461 ggttcggaaa attccgggtc ttttattaac ggtatattag atgcgcttaa tctaaaatta
    11521 aataaggaaa atgcaactaa ccttctcgac aaaatttcca gaccttccaa aacataaatg
    11581 tttagcgttg ggtatttttg cagatgaaaa gcctccgcgc gggattggcg gcttcattga
    11641 ttggcggctc aacggtttaa tttcgcggga aattaaacaa ggaaggatca gtggcgaatt
    11701 tatggaaaag actattattc cctttcctca gcgcattgga acggaatttt tattgctttt
    11761 cggcttaggg aatcttgcgg caattaatta cgatagaatt tacaatgccg cttacctcat
    11821 tgcgggagcc gttgatggta tgtctctccc tagctttgct tttgaccttc atggagaagg
    11881 ccgctgtgat ctagccacct ccggtattgt ggaagcaatg ctcaccggct tttatgattt
    11941 tctatccgca gacaaaaaaa agatctccaa tatggctgcc tgtatcgtta cttctccttc
    12001 ccatcttcaa gaagtatcgc taggcattaa acacttccat gccaactgca aggataaagg
    12061 ctctgtggat gtctgtgcgt tagaaaatag ttttgcctga ggggctgttg aaaaacgctc
    12121 atctgctgcg ttgcgctgca gaccgtacc
//
LOCUS       DASVIM010000197         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1359991, whole genome shotgun sequence.
ACCESSION   DASVIM010000197 DASVIM010000000
VERSION     DASVIM010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359991"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VF305_03970"
     CDS             complement(<1..429)
                     /locus_tag="VF305_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7416321.1"
                     /translation="MFDYIWGKFSNDLAIDLGTANTLVYVKGKGIVLDEPSVVAVHQD
                     ARGMKKVLAVGNEAKAMLGRTPGNIIAIRPMSDGVIADFDVTEAMLKHFILSVHNRKS
                     LVRPRIIISVPSGITQVERRAVREAVESAGAREIYMIEEPM"
     gene            627..2222
                     /locus_tag="VF305_03975"
     CDS             627..2222
                     /locus_tag="VF305_03975"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX7416322.1"
                     /translation="MLRFLRKHARGWFMIAIIVIIIIVFILYFGSGRGSRTANAIAII
                     DGKTISEAEFHNEYGKMLDIARLRLGEKLTPEILKKMDLKQTAYNNLLNRQIIIAKAA
                     DLKVQISDEELRNLIMSSPALQTDGVFDERKYRQILRYNKFTAEDFEALQKIDLIANK
                     IEAIVREGIKISDQEIYDLYAFQNQKINVNFVQISGKDVKKKIIPAQAELEDFLKSNS
                     NLFRIAEQIKIKYIFFAGDSFSPDISESDISDYYSRNRDKYRTKDGKQLSLADAKNAI
                     AKELKKTRGMQSAYVEAKKAHDIIYQEDNFDVYGNKNNLKIHNVEFFPLNKPPQEFTS
                     IKDFAAVFLDLQKNEISKVLTAGNGYYLLKVIDKKPAYLPKLKDIENEVKRRFMESEM
                     QILAGKEAQSILDRTRSGDSLNKIAGENGLKINETGLFQPGNTIPKLGFNQNAAEVLF
                     QLSLKKPYAEKPLFINNAYVILKLKDVSKLDRKDFESKKDLYKKVLISIKRDEAMQAW
                     LEGNKAAMIREKRINIKKEAKDL"
     gene            complement(2269..2344)
                     /locus_tag="VF305_03980"
     tRNA            complement(2269..2344)
                     /locus_tag="VF305_03980"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2309..2311),aa:Ala,seq:ggc)
     gene            2451..2537
                     /locus_tag="VF305_03985"
     tRNA            2451..2537
                     /locus_tag="VF305_03985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2485..2487,aa:Leu,seq:caa)
BASE COUNT          860 a          509 c          511 g          725 t
ORIGIN      
        1 catcggctct tcaatcatgt aaatttctct ggcgccggct gattccactg cctcgcgtac
       61 ggcacgcctt tccacttggg ttattccaga cggtacggaa ataataattc ggggacgcac
      121 caaagacttg cggttatgga cagataaaat gaaatgcttc agcatcgctt cagtgacatc
      181 gaaatcggca atgacgccgt cgctcatcgg cctaatggca ataatattgc caggggtacg
      241 ccccagcatg gctttagctt cattacccac ggcaagcacc tttttcattc ctcttgcgtc
      301 ttgatgaaca gctacaacag aaggctcgtc caaaactatt cctttaccct tgacataaac
      361 cagtgtattg gctgtaccaa gatctatggc cagatcgttg gaaaatttcc cccaaatata
      421 atcgaacagc atttatcctc ctgtcttttc gtaatttaag ataatgactt cccctcgggg
      481 tttattttct atacggttat accgtatttt tttaccctaa tcaacgcctt tttaaattat
      541 gtttgcattt tgcaaatgac tgtaatagta aatcaaaggt ttcggttgct attggacaac
      601 tttattatcc ttttcgaggt gatttcatgc tgagattttt acgtaaacac gcacgtggtt
      661 ggttcatgat tgcaattata gtaatcatta ttattgtctt tattctttac ttcggttctg
      721 gcagaggcag tcgaaccgca aatgcaatag ccataattga cggaaaaaca attagcgaag
      781 ccgaatttca caacgaatat ggaaaaatgt tggatatagc caggctgcgc ttaggcgaaa
      841 aacttactcc ggaaatactc aagaaaatgg acctcaaaca aacggcatat aataatttgc
      901 tgaaccggca gattatcatt gctaaagcag ctgatttaaa ggttcaaatt tccgacgaag
      961 aattaagaaa tttgatcatg tcctcaccgg cattgcaaac agacggcgta tttgatgaac
     1021 gcaaatatcg acaaatactc cgctataata aatttactgc cgaagatttc gaagctctcc
     1081 aaaaaatcga tctaattgca aataaaattg aagcgattgt gcgggaaggc attaaaattt
     1141 ctgatcagga aatttacgac ttgtacgctt ttcagaacca aaaaattaat gttaattttg
     1201 tgcaaatatc cggaaaagat gtcaaaaaga aaattatacc ggcccaagcc gaattggaag
     1261 attttctaaa aagtaatagt aatttattcc gtattgccga acagattaaa attaaataca
     1321 tattttttgc aggcgattct ttttctccag atataagcga atctgatatt agtgactatt
     1381 acagccgcaa cagagataag tatagaacga aggatggcaa acaattatca ttggctgatg
     1441 cgaaaaatgc catagctaaa gaattaaaga aaacaagggg aatgcaaagc gcttatgtgg
     1501 aagcgaaaaa ggcccatgac attatttatc aagaagataa ttttgatgtt tatggcaata
     1561 aaaacaattt aaaaattcat aatgtggaat ttttcccact caataagcca ccgcaagaat
     1621 ttacctccat taaagatttt gcagctgtgt ttttggattt gcagaaaaac gaaatcagta
     1681 aagtccttac tgccggaaat ggctattatc ttttaaaagt tatcgataaa aagcccgctt
     1741 acttgccgaa actgaaagac atagagaatg aagtcaagcg ccgttttatg gaaagtgaaa
     1801 tgcagattct tgccggaaaa gaagcacagt caattttaga tcgtacgagg tcaggagatt
     1861 ctctcaataa aatcgccgga gaaaacggtt taaaaattaa cgaaacaggt ctctttcaac
     1921 caggaaatac tattcccaag ttgggcttta atcagaatgc ggctgaagtt ctttttcagc
     1981 tctcgctgaa gaaaccatat gcggaaaagc cgcttttcat caataatgct tatgtcatat
     2041 taaagctcaa ggatgtaagc aagctggata ggaaagattt tgaatcaaaa aaagacctgt
     2101 acaaaaaggt attgatttcc attaaaagag atgaagctat gcaagcatgg ctggagggca
     2161 ataaagcggc catgattagg gagaaaagaa ttaacattaa aaaggaagca aaagatttat
     2221 aataagaaaa aaagggatga gccaagaaaa acccttccct ttaattattg gtggagctga
     2281 gggggatcga acccctgacc tcatgactgc cagtcatgcg ctctcccagc tgagctacaa
     2341 ccccagtaaa ctactgatta attgtgcagt ccacttaaca taggcattaa ttgcttgtca
     2401 acagatttta gcttgacatt ttgtgacccg actatataat cgctgcccgt gccggagtgg
     2461 tgaaactggt agacgcaggg gactcaaaat cccccgttcg caaggacatc tcggttcgat
     2521 tccgagctcc ggcaccataa taacatcaag ggattgcgca tttataaaca aatgaaaagc
     2581 ccttttattt tttctgctta ttgct
//
LOCUS       DASVIM010000198         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_932277, whole genome shotgun sequence.
ACCESSION   DASVIM010000198 DASVIM010000000
VERSION     DASVIM010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932277"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /gene="lptC"
                     /locus_tag="VF305_03990"
     CDS             <1..343
                     /gene="lptC"
                     /locus_tag="VF305_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX7416323.1"
                     /translation="ATYYKKQNLALFDKVQIKLTMSDGEVYLMTGDKGQILTDKKDLE
                     IKGNVVITSAMGDKFSTDYLNFNDAEKKFYTDAPVTMENKRMKIKGVGLTLFIDKGQL
                     NLSSMVKAKIK"
     gene            356..919
                     /gene="lptA"
                     /locus_tag="VF305_03995"
     CDS             356..919
                     /gene="lptA"
                     /locus_tag="VF305_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEX7416324.1"
                     /translation="MIDRKIYLFVVLFVALLVPASLYAQDSSIVKEDIIKKNEPIEIV
                     SDRMDAFNEKKLVVFSGNAIAKQGNTILKSDKLLLYYKKDPAKKDKIGTKEIEGTGDL
                     EKIEANGNVSLTQGGRIATGDEATYFQDSGQVIMIGNAVLREGKNVIKGDRVIVFINE
                     NRGIVESNTKKQVKAIIYPQEKKEEKK"
     gene            966..1676
                     /gene="lptB"
                     /locus_tag="VF305_04000"
     CDS             966..1676
                     /gene="lptB"
                     /locus_tag="VF305_04000"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX7416325.1"
                     /translation="MAKGLTKIYNNRKVVNQIDLSISPGEVVGLLGPNGAGKTTTFYM
                     IVGLIKPDDGEIFLNGEDISGYPMYQRARKGLNYLPQEPSIFRKLTVEENILAILETL
                     DLREEERREKLQELLGELDLTGLAKNPAYCLSGGERRRVEITRALVTSPKYILLDEPF
                     AGIDPLAVADIQKIIEKLKKKGIGIIVSDHNVRETLSCCDRAYIVNEGAILVEGPPDK
                     IAHSELARKFYFGEDFNL"
     gene            1704..>2535
                     /locus_tag="VF305_04005"
     CDS             1704..>2535
                     /locus_tag="VF305_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEX7416326.1"
                     /translation="MAFELKQSLKLSQQLIMTPQLQQAIKLLQLSRLELVETINQELE
                     ENPLLEEASSDEGNEEEVHAQAEEDIQSIEREDTTKAVERTEELTGEGDGRSEFDWDG
                     YLEDYRPVGVTYGRKDTEAPSWDNVLTESQSLTKYLMWQMKLSSFTEDERRVCTQIVG
                     NLDQNGYLCATVPEIAQLENVSEEFVEAVLKRVQEFDPPGIAARNLQECLLIQARMFG
                     VKNAIIEIIIRDYLKDLELKNYVHIARKLKVPIRDVEIAVLLIGNMDPKPGSIYSEEK
                     I"
BASE COUNT          881 a          426 c          562 g          666 t
ORIGIN      
        1 cgctacatat tacaaaaaac aaaatcttgc tttatttgat aaggttcaaa taaagttaac
       61 aatgtctgac ggtgaagttt atttaatgac gggcgataaa ggccagatac ttacggataa
      121 aaaggacctt gaaataaaag gtaatgtggt aatcacttcc gctatgggtg acaagttttc
      181 tactgattat ttgaatttta acgatgccga aaaaaagttt tataccgacg cgccggtaac
      241 catggaaaat aagcgcatga aaataaaagg tgtggggctg acgctcttca ttgataaagg
      301 acaactaaac ctttcctcca tggtaaaagc aaaaataaaa taactgagaa atgaaatgat
      361 tgatagaaaa atatatttgt ttgtcgtctt atttgttgcc ctgctggtgc ctgccagcct
      421 gtatgctcag gatagtagca tcgttaaaga agatataatt aaaaagaatg aacctattga
      481 aattgtttcc gatcgcatgg atgcttttaa tgaaaagaaa ctggtagtat tttccggtaa
      541 tgcaatagcc aaacaaggca acactatatt gaaatcggac aagttacttt tgtattataa
      601 aaaagacccg gctaaaaaag ataaaatagg cacaaaagaa attgaaggaa ccggggatct
      661 ggagaaaata gaagcaaatg gaaatgtttc gttaacgcaa ggaggaagaa ttgctaccgg
      721 agatgaagca acatattttc aagatagcgg tcaggttatt atgatcggca acgctgtatt
      781 gcgcgaaggc aaaaacgtaa ttaaaggaga tagagtaata gtttttatca atgagaatag
      841 aggaatagtg gaaagtaata cgaaaaaaca agtaaaggcg ataatctatc cccaagaaaa
      901 aaaagaagaa aagaaataat tggagagcat ttagcattta gcgctataaa attatgagta
      961 atttattggc aaaagggtta accaaaattt acaataaccg aaaagtcgtc aatcagattg
     1021 acttatcgat ttcgccgggc gaagtagtcg gtttacttgg ccccaatggt gccggcaaaa
     1081 caaccacttt ttatatgatt gttggattga ttaagccgga tgacggagaa atttttttaa
     1141 acggtgaaga tattagcggg tatcctatgt atcaaagagc gcgcaaaggt ttaaattacc
     1201 tgccgcaaga accgtcaatt ttccgtaagc tcactgtaga agaaaatatc ttggctatct
     1261 tagaaaccct cgatttacgg gaagaagaaa gaagagaaaa acttcaggag cttcttggag
     1321 aacttgatct aaccgggctg gcgaaaaatc ctgcttattg tctctccgga ggcgaaagaa
     1381 gaagagtaga gatcacgcgg gcattagtta cgtctcccaa atacatcctt ttggatgaac
     1441 cttttgccgg cattgatccg ctggcagttg cggatatcca aaaaataatc gaaaaattaa
     1501 aaaaaaaggg aatcggaata attgtttccg atcacaatgt gcgcgaaaca ctttcctgtt
     1561 gtgatcgtgc ttacattgtc aatgaagggg cgatacttgt tgaaggcccc ccggataaaa
     1621 ttgctcacag cgagctggct cgaaaattct attttggtga agattttaat ttataataaa
     1681 ttcgcaattt atgattgttt attatggcgt ttgagttaaa acaaagttta aaactgtccc
     1741 aacagttgat tatgacaccc caactgcaac aggcaataaa attgttgcag ttatctcgac
     1801 tggaacttgt cgaaacaatc aatcaggagc tggaagaaaa tcctcttttg gaagaagcat
     1861 cctcggacga aggaaacgaa gaagaagttc acgcccaagc cgaagaagac attcaatcga
     1921 ttgaacgcga agatacaaca aaagctgttg aacgcacgga agaactcacc ggtgaaggcg
     1981 acggccgctc agaatttgac tgggatggct atctggaaga ttaccgtccg gtaggtgtaa
     2041 cttatggccg caaagatacg gaagctcctt cctgggataa tgttcttact gaaagtcaat
     2101 cgctgacaaa atatttaatg tggcagatga agctttcatc ttttacagaa gatgaaaggc
     2161 gtgtctgcac acaaatcgtc ggtaatcttg accaaaatgg ttatttatgc gcgacggtgc
     2221 cggaaattgc ccagctggaa aatgtcagtg aagaatttgt tgaagctgtt ctgaaaaggg
     2281 ttcaagaatt tgatccgccg ggtatagctg cccgtaattt gcaggaatgt ctgttaatcc
     2341 aagcgcgaat gtttggagta aaaaatgcga ttattgaaat tatcattaga gactacctta
     2401 aagatctgga actgaaaaat tatgttcata ttgcccgtaa attaaaagtt cctatacgcg
     2461 atgtagaaat tgctgtgtta ctaatcggca acatggatcc aaagcccggc agtatttatt
     2521 ccgaagagaa gattc
//
LOCUS       DASVIM010000199         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_847590, whole genome shotgun sequence.
ACCESSION   DASVIM010000199 DASVIM010000000
VERSION     DASVIM010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847590"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VF305_04010"
     CDS             <1..606
                     /locus_tag="VF305_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7416327.1"
                     /translation="VFITASVIFGSLLIGIVFQLLKDLGLTQFIGNVLMGLIVTELSP
                     FFTVLLITLRSGSAINAEMAIMKVNREIKTLETFHIDVVNYLLIPRIINGIISLVLLS
                     TLFSIVLMSSGILFSRIIFGMSIDVYSNILLNSANFSDIVIALVKCACFGFFITLIPI
                     RFGLRASHELTSIPIVVSRGMVSVFTAIMIIEVLSLITKLI"
     gene            762..1064
                     /locus_tag="VF305_04015"
     CDS             762..1064
                     /locus_tag="VF305_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416328.1"
                     /translation="MPRGEAEHIAINALQRLGLGDIAFKRNPDLNNEERFCAMLLRAS
                     MVRDALIIIDRPFKIIPHLKDVDYIFQALKKIEDFYISCHIYDYEWVAEKYGALCQ"
     gene            1061..1822
                     /locus_tag="VF305_04020"
     CDS             1061..1822
                     /locus_tag="VF305_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX7416329.1"
                     /translation="MSREYKVGLFIICTAIVIFLALLYLAREKGFFTTVHTFTLSSKS
                     GEGFTEGMPVVFSGFNIGKVHALELNDKGIVLIKIRIPQRHVKWIRTDSTFILYRPLI
                     GAARIVVTTDNLNSKPLPENKIPEVGIVNDINDTIEKVQPLIEKIIPIAENLERLSRK
                     IDKMADKTDEQVFGKDGTLPQINKILKDIIGKLEKLNKTVDNINKISKDASEGMNDFH
                     MLRSDIDDTVKAIDDVAKKLDAILSKKKEPEVKVP"
     gene            1819..>2188
                     /locus_tag="VF305_04025"
     CDS             1819..>2188
                     /locus_tag="VF305_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416330.1"
                     /translation="MRKILFILLLVFFCACGSSSNQIPQWKDTASTQLENYKKNFLMD
                     KEDAIEPHFAKAKKAVAGSNDLNLFAIAYLTKYALHTAALEDFDDSEFIRIDKLQSIA
                     SNSAYYNFLKGNFAVVKDNLL"
BASE COUNT          666 a          396 c          448 g          678 t
ORIGIN      
        1 gtatttatca cggcatccgt gatattcggg tcactgctca tcggcatagt ttttcaactg
       61 ttaaaggatt tagggcttac tcaatttatt ggcaatgttc tgatgggatt aatcgttact
      121 gaactttccc cttttttcac agttcttttg attacattac gttccggttc tgccattaac
      181 gcggaaatgg ctataatgaa ggtcaataga gaaataaaaa cattggaaac attccatatc
      241 gacgtagtca actatttgct tataccgcgg ataattaacg gcattatatc tttggtctta
      301 ttgagcactc ttttttccat tgtcctgatg agcagcggca ttttgttttc acggataatt
      361 ttcggcatga gtatcgatgt atattcaaat attctgttaa attccgccaa tttttctgat
      421 atagtcatag ctctggtgaa atgtgcttgt tttggatttt tcattaccct gattccgatt
      481 cgcttcggcc tgcgggcttc gcatgaattg acaagcatac ccatcgtagt atcccgcggc
      541 atggtaagtg tttttacggc aattatgatc attgaggtgc tgtcattaat aacaaaatta
      601 atttaaagtt ttttgaaagc gagaaagctc tggatacagg gctgggaaag tttctggaaa
      661 ccaaccggag gcaagcggcg cctgtttcca ttgatatacc ccttatttcc aatcaggaag
      721 tttggctgaa catcgctttg attaaacaat atcatgagaa catgcccaga ggagaggccg
      781 agcatattgc cataaacgca ttacagcggc tcggtttggg ggatattgcc tttaagagaa
      841 accctgattt gaataacgaa gagcggtttt gtgctatgct gctcagggcc tctatggtac
      901 gagatgcttt gataattatt gaccggcctt ttaaaattat cccacacctt aaagatgttg
      961 attatatttt tcaggcgcta aaaaaaattg aagattttta tataagctgt catatatatg
     1021 actatgaatg ggtagcagaa aaatatggtg cgttatgtca atgagccgcg aatataaagt
     1081 cggacttttt ataatttgca cagcgatagt cattttcctt gctctgcttt atctggccag
     1141 ggaaaagggt ttttttacca cagttcatac ttttacactt tcatctaagt cgggtgaagg
     1201 atttaccgaa ggtatgccgg ttgtgttttc gggatttaat atcggcaagg ttcacgccct
     1261 cgaactcaat gataagggta tcgttctcat taaaattaga attcctcaac gccatgttaa
     1321 gtggattaga acagatagca cttttatact ttatcgtcct ttgattggcg ctgcccgcat
     1381 tgttgtgaca acggataact taaatagtaa gccgctaccg gaaaataaaa ttccggaagt
     1441 agggattgtc aatgacatca atgatacaat agagaaagtg cagccgttga ttgaaaaaat
     1501 aatcccgata gcggaaaatt tggaacgcct tagcagaaaa attgataaaa tggcagataa
     1561 aaccgacgag caagtattcg gtaaagatgg aacactgccc cagattaata aaattctcaa
     1621 agatattatt ggcaagctgg aaaaattaaa taagactgta gataacataa ataaaatcag
     1681 taaagatgct tctgagggca tgaatgattt ccatatgctg cgttcggata ttgacgatac
     1741 agttaaagct attgacgatg tggccaaaaa acttgacgcg attctttcca agaaaaaaga
     1801 accggaggtt aaagtgccat gagaaaaatt ttatttatac tgcttttggt atttttttgt
     1861 gcctgtggat caagctcaaa tcaaattccc caatggaaag atacagcttc cacgcaactg
     1921 gaaaattata aaaagaattt tctgatggac aaagaagatg caatagagcc tcattttgcc
     1981 aaagcaaaaa aagctgttgc cggcagcaac gatttgaatc tctttgctat agcttattta
     2041 accaaatatg ccttgcacac ggccgcactg gaagattttg acgacagcga atttatccgg
     2101 atcgataagc tccaatccat tgcttctaat tccgcttatt ataatttttt aaaaggaaac
     2161 tttgctgtcg taaaagataa tttactgc
//
LOCUS       DASVIM010000200         4672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1018517, whole genome shotgun sequence.
ACCESSION   DASVIM010000200 DASVIM010000000
VERSION     DASVIM010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018517"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VF305_04030"
     CDS             complement(<1..1012)
                     /locus_tag="VF305_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX7416331.1"
                     /translation="MLNRPLSTGYRGAIYPVNPRIKEISGLRSYRSILDIPKPVDLVI
                     MTIPARMTPDAMKECVKKGVKGAILITAGFSEVGEKGRAIEDEVVRIAKEGGIRLVGP
                     NCMGILSSSGRLNLSFELMPRTGPISFISQSGTFGVFLSEIANFKGYGLSKFVSIGNQ
                     ADLTATDYLEYLAEDEQTKVIVFYMEGFKDGNRFFELAREVVKKKPILIFKAGRTEAG
                     TRAAMSHTASLAGSDAIFDALCRQAGLIRTDEAMQAFDMAEALTTLPLPPGRRVAILG
                     SGGMGVVTADACASLGLEVPRLKRETIQCLKKIMPPHAPLPTNPIDFAGSARTAIDEA
                     SVV"
     gene            1331..1597
                     /locus_tag="VF305_04035"
     CDS             1331..1597
                     /locus_tag="VF305_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416332.1"
                     /translation="MKIFRQSFLGKSIKSITFPAFSERIMNLVEFLKWIADDIKKFVM
                     ENPGNRLERLDGSPIFKEPLIGFAAGSDFLMVLPLPASFQLDLT"
     gene            1629..3149
                     /locus_tag="VF305_04040"
     CDS             1629..3149
                     /locus_tag="VF305_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7416333.1"
                     /translation="MDTFTLAATSFIIALSLMITGKKDKLHSSFAGLCAAVFISQVGL
                     FLRNIFATGFWIYVEYLGILAIAPFSLRFFRQLTRNKSLLSRGPFIIFILISLSGAVF
                     LFTPLSKLAYLNAAILFYTFFALALCYIALMRYVNKLPLSTEKKRLGYLLVACPIALL
                     VSSINLFSYLGFNVPAINGLIISALLYLILLIIAYPQLHELHDFFARALVIFISTLAG
                     AIIFYLAALFFSNELPSFTSGMMAAFLIAISISPLKMILKKGFSHFYPDSKDVFTSLY
                     EFDEKLEREKALMLAEMAPVFAHEIRNPLGSIKGAAQYLKSEASTEEQQELFNVIIEE
                     ANRLNAVVTQFLDYARPYKLDLKGQNINMIIQKAISIIAANQLAENIAIVRELNHNLP
                     DVEVDEQQFIQVILNIALNAIESMPQSGTLTFRTSNIESGAEGAISITIRDTGSGISK
                     EEIKNIFKPFFTTKKRGVGLGLAICQRIIKEHGGIIRVKSIPAQGTVFLIKLNAAG"
     gene            3204..4568
                     /locus_tag="VF305_04045"
     CDS             3204..4568
                     /locus_tag="VF305_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7416334.1"
                     /translation="MNKILIVDDELNMRLVLSAMLKKEGYEVASASDGREALQILKSS
                     KIAVVVTDLKMPDIDGMELLNHISEQYPEIPVIMITAHGTVATAVEALKKGALDYITK
                     PFDIDELKNVISKAMKTRTLKESELFLPPEEIERTGIIGTSKRTLEIFDAIKRVAPTT
                     TTIMITGETGTGKELVADAIHYNSPRKKNPLIKINCAAIAETLMESELFGYEKGAYTG
                     AAITKPGKFELAHKGTLFLDEVGEIPRDMQVKLLRVIQEQEFERVGGLRTIKVDVRII
                     TATNQNLLQQVQAGNFREDLYYRLNVFPIDVPPLRERKEDIQPLVDYFMDKFNKKLEL
                     SVEVDSKVKEMLLRYEWPGNIRELENLIERMMLLAKDNLITSQEVPAEFKAALDKIIT
                     APVDDGKKPFKDYMRDHVENVERQMIIKCLEESGGNVTNAAKQLGLSRKGLQLKMIKY
                     NLRK"
BASE COUNT         1326 a         1077 c         1009 g         1260 t
ORIGIN      
        1 ccacgactga ggcctcatca atggcggttc gagcgcttcc cgcaaaatcg atcgggttag
       61 tcggcagggg agcatgagga ggcattatct tcttcagaca ctgaatagtt tcccttttca
      121 ggcgggggac ttccagccct aaggaggcgc aggcgtctgc ggtgaccaca cccatgccgc
      181 cgcttcccaa tatagctacc cgccttcccg ggggaagagg caaggtagtc agcgcttcgg
      241 ccatatcaaa ggcctgcata gcctcatctg ttctgataag accagcctgt cggcataggg
      301 catcgaatat tgcatcggat ccggcaaggg atgcagtatg agacatagcg gctctggtcc
      361 ccgcttcagt gcgccctgct ttgaaaatca gtatcggctt tttcttgacc acttctctcg
      421 ccagttcgaa gaagcggttg ccgtccttga aaccctccat ataaaataca ataaccttgg
      481 tttgctcatc ctcggccaga tactccaggt aatcggtagc ggtaaggtcc gcctggttcc
      541 cgatgctcac gaacttgctg agcccgtacc ctttgaaatt ggcaatctcg gagagaaaaa
      601 cgccgaaggt gccgctctga gagataaagg aaataggtcc tgtacggggc atcaactcaa
      661 atgacaggtt aagacgtccg ctggaactca atatccccat gcagttgggc ccaacgagtc
      721 gaatgccccc ttccttggca atccggacta cttcatcttc tatggccctg cccttttccc
      781 ccacctcgga aaagccggca gttatgagga tagcgccctt gacccccttt ttcacgcact
      841 ctttcattgc atccggcgtc attcgagccg ggatagtcat aatcaccaga tccaccggtt
      901 tggggatatc cagaatactc cgataggatc gaagtcctga tatttctttt atcctcggat
      961 tcacgggata tattgcgccc cggtatcctg tactcaaggg tctgttcagc atcctccctc
     1021 cccatttccg tggtgagtca gatgcgccga ttatggcaat ggattctggt ttaaatatgg
     1081 gatcaagttg ctcagtaata ctcttcaaaa gttcctccgt tgaatttgtt aaatcaaatg
     1141 cagattttta atttcctgta agtagacagt atagctaaca tctacaagtg caaaaattcg
     1201 gttaagcatt tcataatagt tattaaaaat aaagggctat aaatcaaatc ttatatcggc
     1261 ttgtttcccg gttggaaata ccccccctca gaatcatgcg gacaacgcac atcaacaaca
     1321 gcaaaaatat atgaaaatat ttcgccagag tttcttaggg aaaagcataa agtccattac
     1381 ctttccagca ttttctgaac ggataatgaa tttagtagaa tttttaaaat ggattgctga
     1441 tgatattaaa aaatttgtaa tggagaaccc gggcaacagg ctagagcgcc tggatggttc
     1501 tcccattttt aaggaaccgc ttatcggatt tgcggctggt tctgatttct taatggtatt
     1561 gcctctgccg gcaagcttcc agcttgactt aacctaacat tattaatatc atcctggtga
     1621 gggtaattat ggatacattt actttagccg caaccagttt tatcattgct ctttcgctca
     1681 tgattaccgg caagaaggat aagctgcatt catcttttgc cggcctttgc gccgccgtct
     1741 ttatttcgca agtggggctt tttctgcgca atatctttgc taccggattt tggatatatg
     1801 tcgaatatct gggaattctg gcaattgctc ccttctctct gcggtttttc cgccagctaa
     1861 cccgtaacaa atctctcctt tcacggggac cgtttattat tttcatctta ataagtttgt
     1921 cgggtgccgt tttccttttt acaccattat ctaaattggc gtatttgaat gccgccatat
     1981 tattttacac ctttttcgct ctggcacttt gctatatcgc cctgatgcga tatgtaaata
     2041 aattaccact cagcaccgaa aaaaaacgtt tggggtactt gctcgttgct tgccccattg
     2101 ctttgctcgt aagcagtatt aatctattca gttatttagg attcaatgta ccggcaataa
     2161 acggcctgat catatcagcc ttgctttatt taattttgct gatcattgct tatccgcagc
     2221 tccatgaact gcatgacttt ttcgcccggg cgctggtaat ttttatcagt actcttgccg
     2281 gcgcaattat tttttatctg gcagccttat ttttcagcaa cgaactgcct tcttttacca
     2341 gtggaatgat ggcagcattt ttaatcgcca tttccatttc gccactaaaa atgattttga
     2401 aaaaaggttt cagccacttt taccccgaca gcaaagatgt tttcacttct ctttatgaat
     2461 ttgacgaaaa gctggaaagg gaaaaggcct tgatgctggc ggaaatggcg ccggtttttg
     2521 ctcacgaaat ccgcaatccg ctgggttcga tcaaaggtgc agcccagtat ttgaaatcgg
     2581 aagcatccac cgaagaacag caagaactct tcaacgtcat tattgaagaa gccaacagac
     2641 tcaatgccgt tgtcactcag tttcttgatt atgcccggcc atacaaactc gatcttaaag
     2701 gtcaaaatat caacatgatt attcaaaagg ctatctccat catcgcggcc aatcagctgg
     2761 cggaaaatat tgctattgtc cgggagttga atcacaacct gcccgatgta gaagtagatg
     2821 aacagcaatt tatacaggtt attttaaata tcgctctcaa cgcgatcgaa tcgatgccgc
     2881 agagcggcac tttaactttt cgcacatcca acatcgaatc cggagccgaa ggagcaataa
     2941 gcataacgat tcgcgatacc ggaagcggaa taagcaaaga ggaaataaaa aatattttca
     3001 agccattttt cacaaccaag aaaagaggtg ttggacttgg cctggctatt tgccaaagaa
     3061 tcatcaaaga gcacggcgga atcatccggg taaaatcaat tcctgcgcaa ggaactgtat
     3121 tccttatcaa gctcaatgca gccggctaaa ctttaaacac aatccaactt gatttaaatg
     3181 ctaaagcata ataattgtaa accatgaata aaattttaat cgttgacgat gaattaaata
     3241 tgcggctggt gttgtctgcc atgctcaaaa aagaaggcta tgaagttgct tccgcctccg
     3301 acggacggga agctctgcaa attttaaaat caagtaaaat agccgtggtt gtaaccgatt
     3361 tgaaaatgcc ggatattgac ggcatggaac tgctcaacca catttctgaa cagtatcccg
     3421 aaattcccgt gattatgatt accgcccacg gcactgttgc tacagctgtt gaagctctaa
     3481 agaaaggcgc gctggattat attaccaaac cgtttgacat cgatgagcta aaaaacgtca
     3541 tttccaaagc tatgaaaacg cgcactctaa aagaaagcga acttttcttg ccgccggaag
     3601 aaattgaacg caccggtatt atcggcacaa gcaaacgaac gctggagata tttgacgcca
     3661 tcaaacgggt ggctccgacg acaaccacaa tcatgattac cggagaaacc ggcaccggca
     3721 aagagctggt agccgacgct attcactaca actctccacg caaaaaaaat ccgctcatta
     3781 aaatcaactg cgccgcgatc gccgaaacgc tgatggaaag cgaactattc ggttatgaaa
     3841 aaggcgccta caccggcgcc gctattacga agccggggaa attcgaattg gctcataaag
     3901 ggactttgtt tcttgacgag gtgggagaaa ttccgcgcga tatgcaggta aaactcttac
     3961 gcgttattca ggagcaggaa tttgaacgag tgggcggatt gcggacgata aaagtagacg
     4021 tgcgtatcat taccgccacc aatcaaaatc ttttgcagca ggtgcaggcg ggaaattttc
     4081 gtgaagattt atactatcgt ctcaatgttt ttcctattga cgtcccaccg ctgagggaaa
     4141 gaaaagaaga tatccaacct ctggttgatt attttatgga taaatttaac aaaaagctgg
     4201 agctttcggt tgaggtggac agcaaagtaa aagaaatgct tttgcgctat gaatggccgg
     4261 gcaacatccg tgaattggaa aacctcatcg aaagaatgat gctgctggca aaggacaatc
     4321 tgattacatc ccaggaagtc cccgctgaat tcaaagctgc tttggataaa atcataacgg
     4381 cgcctgtaga tgacggcaaa aaacctttta aagattatat gcgcgatcac gtggaaaatg
     4441 ttgaaaggca gatgattatc aagtgcctcg aagaatccgg cggtaatgtt accaacgcag
     4501 ctaaacaact ggggctaagc cgcaagggat tgcagttgaa gatgatcaaa tacaacctgc
     4561 gcaaatgatt aaaattcatt attctgacaa aattagaata gtttattgac atcagttaat
     4621 tcggcgtgct aacgttttaa aaaaaattaa ggagaacgct atgaacatat at
//
LOCUS       DASVIM010000201         5560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1445566, whole genome shotgun sequence.
ACCESSION   DASVIM010000201 DASVIM010000000
VERSION     DASVIM010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445566"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /gene="proC"
                     /locus_tag="VF305_04050"
     CDS             <1..618
                     /gene="proC"
                     /locus_tag="VF305_04050"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX7416335.1"
                     /translation="IIIIAVKPQNMAATLTEFSSVIDTSKVVISIAAGIATNFIETSL
                     AKGARVLRVMPNTPALVGEGAAAVARGSCAKVDDVNLTRAIFDAVGISVEVNENLMDA
                     VTGLSGSGPAYCFMIIEALIDAGVKMGLPHDLAAKLAAQTMLGAARLCLQSDKQPAQL
                     REMVTSPGGTTAAGLKVLEEGNIRATIISAVEAATKRSKELASGK"
     gene            complement(901..2727)
                     /locus_tag="VF305_04055"
     CDS             complement(901..2727)
                     /locus_tag="VF305_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX7416336.1"
                     /translation="MRHTMLVNAVHKEQMRMATVNEKGRLIEFNIEMAVREPITGNIY
                     KGKVLKVERGLQAAFVDYGGGKDGFLPLRDVSPEYLTELENGQAGGKAVLKAGQEIIV
                     QAVREVIGNKGALLTSYISIPGRYLVLLPNKPGGGISRKIESEEDRKKIKNLMEQIKV
                     PEDMGFIVRTAGINRTKQEILRDYQLLMRLWKEIYKKASSINAPLLIYQETDFGVRSL
                     RDYLTPEIDEILVDNQDTFREMRAYLKAVAPRHLRIIKHYKEKTPIFDRFQLEEQIRV
                     IYQERVEIKSGGYIIINPTEAMITIDVNSGRGSNKKNIEETAFKTNIEACEEIARQLR
                     LRDLGGLIVIDFIDMIDKKHIAEVEKSFKKALSIDRARIQLSRISKFGMLELSRQKKQ
                     STIQEISYTNCPYCKGTGLRPSLEYAALGAFRKIESEVVKGIYSELRIILPHEIASYI
                     LNQKRSELLNLESVFGLTLHISGSTEMSWDKLDIQQIIKEQVVEKPAKQEELPLETAE
                     NPVLKDEQKEPVKKKGRRRPRHKKRRPTEIKTGDSLPANIPPIEAPVTPAGENLPVAL
                     NPPVESIPLIIAPVEPKEIIVAPEPSEPAIPISETGKKEGNV"
     gene            complement(2826..3431)
                     /locus_tag="VF305_04060"
     CDS             complement(2826..3431)
                     /locus_tag="VF305_04060"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX7416337.1"
                     /translation="MLSIHSQNPQMRLIKIAVDVLRQGGVIIYPTDTVYGLGCDLSNK
                     KGIERIYELKKRNKKKPLSFVCSDLKHISQYAKVTDYAYKTMKRLLPGPYTFILEASR
                     LVPKIILPKRPTIGIRVPDNQICLALIRELGQPIISTSVKTAEGEDLGNPYVIEEHFG
                     RIIDLIIDGGNIIPEPSSVIGLVDDNVEIIRIGKGDVSAFL"
     gene            complement(3673..5256)
                     /gene="cimA"
                     /locus_tag="VF305_04065"
     CDS             complement(3673..5256)
                     /gene="cimA"
                     /locus_tag="VF305_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX7416338.1"
                     /translation="MTRNQILIYDTTLRDGTQGEQTNFSAEEKLRIAQRFDETHFHYI
                     EGGWPGSNPKDVRFFEMAKKVKFKNSRLTAFGSTRRPHTKPEACPNLKALVKAGTPAC
                     ALVGKSWDLHVTEILKIDLDENLAMITDSIEYLKSMGKEVLFDAEHFFDGYKNNPRYT
                     TKVVKTALAAGADMIILCDTNGGSMPHEISAIVKKISSSVPLDIAGIHTHNDCGLAVA
                     NSIVAVQEGVKMVQGTINGYGERCGNADLISVVANLQLKMGRHCLPTESIQQLTNLSH
                     YVSDVANVPPLNSRPFVGRSAFAHKGGVHVSAVVKNALAYEHIQPELVGNHRRVLVSD
                     LAGKSNVEYKAKELGIDLAKNDALSKKIVHEVKLMEDKGFQFDAADGSLSVLIKKAIG
                     EFVEPFTLESFNVVTSRSEDKPCLSQATIKISVDGEEELTAAEGNGPVNALDHALRKA
                     LTKFYPQIKEMHLVDFKVRTLEGSEGTAASVRVLLDSTDGEDLWSTIGVSENIIEASW
                     QALVDSIEYKLSKDKNNNK"
BASE COUNT         1429 a         1262 c         1188 g         1681 t
ORIGIN      
        1 ataatcatta tcgcagtcaa accgcaaaac atggccgcaa cattaacgga attcagctca
       61 gttatagata cttcaaaagt cgtgatttct atcgctgccg gaatcgcaac caattttatt
      121 gaaacatctt tggccaaagg cgcccgggtt ttgagagtaa tgcccaacac ccccgcgctg
      181 gtaggggagg gagcggcggc ggtggccagg ggaagttgtg ctaaggttga tgatgtcaac
      241 cttacgcgcg ctattttcga tgccgtggga atcagcgtcg aagttaatga aaatctgatg
      301 gatgccgtga ccggactctc cggtagcgga cccgcttatt gttttatgat catagaggcg
      361 cttatagacg ccggagtaaa aatggggttg ccgcatgacc tggcggcaaa actggcagca
      421 caaacaatgc tgggagccgc ccgtctctgc ctgcaaagcg ataagcaacc agcgcaacta
      481 agggagatgg tcacatcgcc gggaggcact actgccgccg gattgaaggt gctggaagaa
      541 ggaaatattc gcgcgacaat tatttccgct gtggaagcgg cgaccaagcg atctaaagaa
      601 ctggccagcg gcaaataaat aattccaatt ctaaatcaaa gcgctatctt tgttggcgtc
      661 gttgtcggct caatatcatc tttgatttag gattggtata ataggtagaa gccagaaatt
      721 tatgctgaat tattttttac ccggcaattc taatacaacg ccaatcactg cgccgattat
      781 tgctctagat aagccgtatt ttagcacaag ttcgtttaag aacttccttg gggatatata
      841 aacgccagtt atcagattct cggtgtgcgt cgtaattcag aacaaaaaat ttaaattttc
      901 ctaaacattt ccttcttttt tcccggtttc actgatggga attgccggtt cgctaggttc
      961 tggagcaaca attatttctt ttggttcaac cggtgcgatg ataagcggaa ttgactctac
     1021 cggaggattt agcgcaaccg gtaaattttc gccggcaggg gtcaccggag cttctatcgg
     1081 cggtatattt gccggcagtg agtcacccgt ttttatttct gttggtcttc tttttttatg
     1141 gcgaggtctc cggcgtccct tcttttttac aggctctttt tgctcatctt ttaataccgg
     1201 attttcggct gtttccagag gaagttcttc ctgtttggcc ggcttttcca caacttgttc
     1261 cttgatgatt tgctgtatgt ccagtttgtc ccaggacatc tccgtactgc ccgaaatatg
     1321 caaggtgagg ccgaaaaccg actccaaatt taaaagctcg cttctttttt gattgagaat
     1381 gtagctggca atttcatgcg gcaaaattat tcttaactcg gaataaatgc ctttgaccac
     1441 ttcggattca attttgcgaa acgctcccag agcggcatat tccaaagatg gacgcagacc
     1501 ggtgcctttg cagtaagggc agttggtgta acttatttcc tgaattgttg actgtttctt
     1561 ttgccgcgat aattcgagca ttccgaattt agaaatacgt gaaagctgga ttcttgctct
     1621 gtcaatgctc agcgcttttt tgaacgattt ttctacttcg gcaatatgtt ttttatcgat
     1681 catgtcaata aaatcaatga cgatcaaccc gcctaaatcc cgtaagcgca attgccgggc
     1741 aatttcttcg caggcttcaa tattggtctt gaaggcggtt tcctcaatat tttttttgtt
     1801 ggaaccgcgg ccggagttca catcaattgt aatcattgct tccgtcggat tgattataat
     1861 gtacccgccg gatttgattt ccactcgctc ctgataaata acgcgtattt gttcttccaa
     1921 ttggaatcgg tcaaaaatag gagtcttttc tttgtaatgt ttgataattc ttaaatgacg
     1981 aggagccacg gctttcaaat aggcgcgcat ctcacgaaat gtatcctggt tgtcaaccag
     2041 aatttcgtct atttccggcg ttaagtaatc gcgcaggctg cgcacgccaa agtcggtctc
     2101 ttgataaata agtagaggcg cattaatact actagccttc ttatatattt ctttccaaag
     2161 gcgcatcagg agctggtaat cgcggagaat ttcttgcttg gtgcggttga tcccggctgt
     2221 ccgcacgata aatcccatat cttcgggaac tttaatttgc tccatgagat tttttatttt
     2281 tttccggtct tcttcacttt ctatcttccg tgatatgcca ccgccgggct tgttgggaag
     2341 caggactaaa tatctgccgg gaatagaaat ataggaagtt aaaagagcgc ctttattgcc
     2401 gattacttct cttacagctt gaacaatgat ctcctgacct gcctttagca cagctttgcc
     2461 gccggcttgg ccgttttcca gttcggttaa atattcgggg ctcacatccc taaggggaag
     2521 aaacccatcc ttgcctccgc cgtaatcgac aaaggccgct tgcaggccgc gttcaacttt
     2581 cagcaccttg cctttgtaga tattgccggt aatgggttct cttaccgcca tttcaatgtt
     2641 gaattcaatg agcctgcctt tttcattaac ggtagccata cgcatctgtt ctttgtgaac
     2701 agcgttgact agcatagtgt gtctcataaa tgtccttttt ggccggctgc tatttttgtg
     2761 cgtcctcgca cacagataga cttcttagtg tctattcgcc aaatagacgg cgcgaccggt
     2821 tttatttata gaaaggcgct cacgtcgcct ttgccaattc ttataatttc aacattgtca
     2881 tcaacaaggc cgatgacgct cgatggttcg ggtataatat taccgccgtc aataatcaaa
     2941 tcgataatcc ggccaaagtg ttcttctatt acgtaaggat tgcccaaatc ctctccctcc
     3001 gccgttttta cactggtgct gattatcggt tgtcccagtt ctctgatcaa ggccaggcaa
     3061 atttggttat ccggcactcg aattcctatt gtcggccttt tcggtaaaat tattttaggc
     3121 accaaacgcg atgcttccag aataaaagtg taaggccccg gcaaaagccg cttcatcgtt
     3181 ttgtaagcat agtcggtaac tttggcgtat tggctgatat gtttcaaatc cgagcaaaca
     3241 aaactcaacg gctttttttt atttcttttt tttaactcgt aaattctttc aatccctttc
     3301 ttattggata aatcacagcc caaaccgtaa accgtgtcgg taggataaat tattaccccg
     3361 ccctgacgca agacatccac agctatctta atgagacgca tttgcggatt ttggctgtgg
     3421 atagataaca tagcttaatt cccaattatt ttatgccact tttcgggaaa tgtatatcaa
     3481 gaattttgaa cttaagcaat ggaagcttct tcggagagca gggataaaat cagtacgtgg
     3541 aaacagagtt tcatacaatt aaggatatcg aggcaacccg gcatgcccgg catacgccgg
     3601 gtaaactgcc gggggaacct gcccggtggg ctttgtcagc aaagatagcc gaacgaaagc
     3661 ggcttgctat gattatttat tgttgttctt gtccttgcta agtttgtatt cgatactatc
     3721 aacaagagcc tgccaactgg cttcaataat attttcggaa acaccgatgg tactccatag
     3781 atcttctccg tccgtggaat ccaaaagtac ccgaacactc gcggctgtgc cttccgaacc
     3841 ttccagcgtg cgcactttaa aatcaaccaa atgcatctct ttaatttgcg gataaaattt
     3901 tgtgagcgct ttgcgcagtg cgtgatcgag ggcattcacc ggaccgtttc cctcggcggc
     3961 tgtcaattct tcctcaccat caaccgatat tttaatagtg gcttgggaaa gacaaggttt
     4021 gtcttccgag cgtgaagtaa cgacattaaa actttccaga gtaaaaggct caacaaactc
     4081 gccaatcgcc tttttaatca aaacggaaag cgagccgtcg gcagcgtcaa attgaaatcc
     4141 tttgtcctcc ataagcttga cttcatgcac tattttttta ctaagagcat catttttagc
     4201 gaggtcaatg cccaattctt tggctttgta ttcaacattg cttttacccg ctaaatcgga
     4261 aaccaggacg cgccggtggt tgccgacaag ttccggttgt atatgctcgt aagccagggc
     4321 atttttaact acggcactaa cgtgaacacc acccttatgc gcaaaggcgc tacgccccac
     4381 aaacggtcgc gaatttagcg gcggaacatt agcgacatcg ctcacataat gagaaagatt
     4441 ggttaactgc tgaatggatt ccgtcggcaa acaatgtctg cccattttaa gttgtaaatt
     4501 ggcaacaacg gaaatcaagt cagcattgcc gcatctttct ccgtaaccat tgatagtgcc
     4561 ctgaaccatt ttaacacctt cctgcacggc gacaatggaa ttggccacgg ccaggccgca
     4621 atcattatgc gtatgaatac cggcaatgtc caaaggaacg gaagaagaaa ttttttttac
     4681 gatagcacta atttcatgag gcatggaacc gccgttcgta tcacaaagaa taatcatatc
     4741 cgcaccggca gccagcgccg ttttcacaac tttagtcgtg taacggggat tatttttata
     4801 gccgtcgaaa aaatgctcgg cgtcaaaaag aacttctttg cccatcgatt tcagatattc
     4861 aatggaatcg gtaatcatgg ctaaattttc atcaaggtct atttttaaaa tttccgtcac
     4921 gtgcaaatcc catgattttc cgactagcgc acaagcagga gtgccggctt tgacaagagc
     4981 cttcaaatta gggcatgctt ccggttttgt atgcggacgg cgggtagagc cgaatgccgt
     5041 caaccgtgaa tttttaaatt taactttttt tgccatttcg aaaaagcgca cgtctttagg
     5101 attggaaccc ggccagcccc cttcgatgta atggaaatgt gtttcatcaa agcgctgcgc
     5161 tatgcgcagt ttttcttccg ccgaaaaatt ggtttgttct ccctgtgtcc catctcttaa
     5221 agttgtatcg taaatcaaaa tttgatttct tgtcattttg tcctcacttt caaaaaaaat
     5281 ccgctgccgg gttttcctgg cagcggattt cgagtttgtt tgattataaa ttgctactat
     5341 tccacgacca agcctcttat ggggaagcta attatgatga tgctaattat aatttcacta
     5401 ataatgatcg tgttcatata aaatttttgc ttcgctttcg tttgtaaaaa ttaagtctca
     5461 ttaataaata tttaattttt ctgtataata attttttaat agttttttgt caatagaaaa
     5521 taatttcttt aatcaaatat gtccaccagt aaagaagatt
//
LOCUS       DASVIM010000202         5326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1701967, whole genome shotgun sequence.
ACCESSION   DASVIM010000202 DASVIM010000000
VERSION     DASVIM010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701967"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VF305_04070"
     CDS             <1..445
                     /locus_tag="VF305_04070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7416339.1"
                     /translation="EKSFNELMIFLREQRSLLAVTPNIWPVKGWVTSEFGIRESPFTS
                     GVEFHKGLDIATRFGKEVVAPADGLVIVVSELTQDGNLIKIDHGHGLVTAFAHLSKML
                     VKQGLRVKRGDVIGYVGDTGRSTGSHLHYAVFVNKVPVNPRRYLR"
     gene            542..3061
                     /gene="secA"
                     /locus_tag="VF305_04075"
     CDS             542..3061
                     /gene="secA"
                     /locus_tag="VF305_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX7416340.1"
                     /translation="MLGNILKKIVGTKNDRELKRLSLLLDEVNNFEPTMMSLNDEELR
                     EKSAYFKEKLKNGLTLDDILTEAFAVAREASRRTLLMRPFDVQVIGGIVLHEGTIAEM
                     KTGEGKTLAATMPLYLNALEGKGCHVVTVNDYLAKRDSEWMGPIYHFLGLSVGVIVHG
                     MDDDERREAYNADITYGTNNEFGFDYLRDNMKFSLEDYVQREFNYAIVDEVDSILIDE
                     ARTPLIISGPSEESTDKYYKINQIIPRLKKDIDYTIDEKSRTVVLTEEGVSHIEEYLN
                     VQNLYEPRNIEIVHHVSQALRAHTLFKRDVDYLVKDGEVIIVDEFTGRVMPGRRYSDG
                     LHQALEAKEKVKIERENQTLASITFQNYFRMYKKLAGMTGTADTEAAEFKKIYNLDVV
                     VVPTNMPMIRKDNNDLIYKTEKEKIKAVIEEVKALNKAKRPVLIGTISIEKSELLSKH
                     LTRAGVKHHVLNAKNHEREAEIVAQAGQPGMVTISTNMAGRGTDIRLGEGVAEIGGLH
                     ILGTERHESRRIDNQLRGRSGRQGDGGSSRFYLSLQDDLLRIFGADRISSIMDRIGIE
                     EDQPIEHKYISRAIENAQKRVEGQNFDIRKHLLDYDDVMNRQRKVIYEQRKKVLRGDN
                     LWSDIQEMVEEIVDDLLIEYIDEKGHADDLNFKGLDDAIFKQFNLKLNLANSDQTASA
                     QPIREVIIENALNLLREKEKNFGKELMNYLMKVIMLQAIDDHWKEHLLAMDHLREGIG
                     LRGYAQKDPVREYQREGYEMFMEMIFHIKMDTLEKLCLVKIQREEEVEEIRQKQKQDY
                     IMSRGEDVPTVQTAKREGVKVGRNEPCPCGSGKKYKKCCGA"
     gene            3086..4285
                     /gene="argJ"
                     /locus_tag="VF305_04080"
     CDS             3086..4285
                     /gene="argJ"
                     /locus_tag="VF305_04080"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX7416341.1"
                     /translation="MLKKTNRISVPGFLANGISVGIKGSERKDLALIYSTIPAKVAAV
                     FTKNSFKAAPVLINAGRVKKGIAQAIITNSGCANALTGKEGYKDALAVSAALSTQLKI
                     RDELVLIGSTGIIGEKLPVKKITGGIGKLVKGLNEFGIEDAETAMMTTDKYPKIAIRK
                     GIVGAKDITICGIAKGAGMIEPNMATLLTYVITDALIDSKALNTVFHQAIDATFNSIS
                     VDGCMSTNDMAIILANGLAGNNPLERAQARLQRFRDMLCDVLKELCQAVVQDGEGATK
                     FISVIVEGAKSKSDARRAAYAIANSNLVKTAFFGEDPNWGRIIAALGSSGIPLEKEKV
                     SLSIGNMTVYSQDSPVNINLNKLKEIFQKDRIDVRVGLGGGDKSYCVYTSDLSYDYVK
                     INAEYSS"
     gene            4464..5291
                     /gene="rpsB"
                     /locus_tag="VF305_04085"
     CDS             4464..5291
                     /gene="rpsB"
                     /locus_tag="VF305_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX7416342.1"
                     /translation="MSAISMKLLLEAGVHFGHQTNKWNPKMKQYIFGARNNIYIIDLQ
                     QTVTMFQTAYNFVVDTVSQGKEILFVGTKKQSQESIKEEAIRCGMPYVNQRWLGGMLT
                     NFFTIKKSIDRLNTLTIMFSDESIKAFPKKEITKLQKEKEQLENVLGGIRNMKSHPAA
                     VFIVDPNREHITVNEAKKLKIPIVAIVDTNCDPDDIDYVIPGNDDAIRAIKLFASKFA
                     DAVLEGKKRFEEKLNAESNKEAGTVVETKSVETDIPESIEMEGKTADSEEYRDESHT"
BASE COUNT         1764 a          900 c         1230 g         1432 t
ORIGIN      
        1 agaaaagagt tttaacgagt taatgatttt tttacgagaa caaaggtctc ttcttgctgt
       61 tacacctaat atatggccgg ttaaaggatg ggtaacatca gaatttggaa taagagaatc
      121 acctttcact tcaggagttg aatttcataa aggattagat atagcaacta gattcggcaa
      181 agaagtagtt gccccggcgg atggacttgt aattgtggtt tccgagctta cgcaagatgg
      241 taacttaatt aaaatcgacc atggtcatgg cttggtcact gcttttgccc atttgtcaaa
      301 gatgcttgta aaacaagggc taagagtcaa aagaggtgac gtaatcggct atgttggcga
      361 taccggccga agcacaggtt cgcatcttca ctacgctgtt tttgtcaata aggttccggt
      421 caatcctaga agatacttga gataatcggt tgataggaaa cacaaaccga aataatcgcc
      481 gtatagtgaa aatgattaaa ttcaccggta aagttaccgg ttttttttta gaaggtaatt
      541 aatgctggga aatatactga aaaaaattgt ggggacaaaa aatgatcgag aattgaagcg
      601 attgtcattg cttttagacg aggtcaataa ttttgaacca acgatgatgt ctttgaacga
      661 tgaagaatta cgggagaaat ctgcatattt taaggaaaaa ttgaagaatg gtttaactct
      721 tgatgatatt ctgaccgaag cttttgctgt agctagagaa gcttcacgcc gcactttgtt
      781 gatgcgccca tttgatgttc aggtaatcgg tggaattgtt ttacatgaag gcactattgc
      841 cgagatgaaa acaggtgaag gaaagacact ggctgcaacc atgccgctat atcttaatgc
      901 gctggaagga aaaggctgcc atgttgttac cgtaaatgac tatctggcca aaagagactc
      961 agaatggatg gggccgattt atcactttct gggtttaagt gtaggcgtta ttgtccatgg
     1021 tatggatgat gatgaaagaa gagaagcata taacgctgac ataacttacg gcactaataa
     1081 cgaatttggt tttgattatc ttcgtgacaa tatgaagttt agtctggagg attacgttca
     1141 acgtgaattc aattatgcta tcgttgacga agtagacagc attctgattg atgaagcccg
     1201 gactccatta attatctccg gtccgtccga agaatcgacg gataaatact ataaaataaa
     1261 ccaaataatt ccgagactga aaaaagatat agattacacg attgatgaaa agagccgtac
     1321 ggttgtttta acggaagaag gcgtgtctca tattgaagaa tatttaaatg tacaaaatct
     1381 ttatgaaccg cgaaacatag aaattgttca tcacgttagt caagcgctcc gcgctcatac
     1441 tttatttaag cgggatgtgg attatctcgt caaagatggg gaagttatta ttgttgacga
     1501 attcaccgga agggtcatgc ctggcaggcg ttacagtgat ggtttacatc aggccttaga
     1561 agcaaaggaa aaggtaaaaa ttgaaagaga aaatcaaact ctggcttcaa tcacatttca
     1621 aaattatttt agaatgtaca agaaactggc cggtatgacg ggaaccgccg atacagaagc
     1681 ggcagaattt aagaaaattt acaatttgga tgtcgttgtc gtaccgacca atatgccgat
     1741 gattcgcaaa gataataatg atcttattta caaaactgaa aaagaaaaga ttaaggctgt
     1801 tatcgaagaa gtcaaggctc tgaataaggc gaaaaggccg gtgctgattg gaactatttc
     1861 catcgaaaaa tcagaactct taagtaaaca tcttactcgt gccggtgtta agcaccatgt
     1921 tctgaatgcg aaaaatcatg aacgggaagc ggaaatagtt gcccaggccg gtcagccggg
     1981 tatggttact atatccacta atatggccgg tcgcgggacg gatattcgtt taggcgaagg
     2041 ggttgccgaa ataggcggcc tgcatatttt aggcaccgaa cgtcacgaaa gccgccgtat
     2101 cgacaatcaa ttaagaggcc gctccggccg gcaaggcgat ggcggttctt cccgctttta
     2161 tctttccttg caggatgatt tgttgcgtat attcggggca gatagaatat cgtcgattat
     2221 ggacagaatc ggtattgagg aagatcagcc tattgaacat aaatatattt ctcgtgctat
     2281 tgaaaatgcc caaaagcgtg tagaaggaca aaactttgat atccgtaagc atcttttaga
     2341 ttacgatgat gtgatgaaca gacaacggaa agtaatctat gaacaacgta aaaaagtatt
     2401 gcgtggagat aacttatggt cagacattca agaaatggtt gaagaaattg tcgatgacct
     2461 tttaatcgaa tacatagatg aaaaaggtca tgccgatgac cttaatttta aaggacttga
     2521 tgacgccata tttaagcaat tcaatttaaa gcttaatctg gctaattcag accaaacagc
     2581 ttccgctcag cctatccgag aagttataat tgaaaatgcc ctaaatcttt tacgagaaaa
     2641 agaaaaaaat ttcggcaaag aattaatgaa ttacttgatg aaagtaatca tgcttcaggc
     2701 aattgatgat cactggaaag aacatttatt ggctatggat cacttgcgag aaggaattgg
     2761 tctgcgtggc tacgcccaaa aggatcctgt tagggaatat cagcgtgaag ggtacgaaat
     2821 gtttatggaa atgattttcc atattaaaat ggatacattg gagaaattat gtttggttaa
     2881 aatacagaga gaggaggagg tagaagaaat acgacagaaa caaaaacaag attacattat
     2941 gagccgtgga gaagatgtgc caactgtaca aacagctaag cgtgaaggag tcaaggttgg
     3001 aagaaatgaa ccttgccctt gtggaagcgg taaaaaatat aaaaaatgtt gtggcgctta
     3061 agttatttaa agaggagtaa gctcaatgct taaaaaaaca aatagaatta gcgtccccgg
     3121 ttttctagcc aatggaattt ccgtaggaat taaaggctcg gaacgaaaag acctcgcctt
     3181 aatttattcc actataccgg cgaaagtagc ggctgtattt acaaaaaatt cttttaaagc
     3241 tgcaccagta ttgattaatg ctggacgtgt taaaaaagga atagcccagg cgataattac
     3301 caatagtggc tgtgccaacg ccttaaccgg aaaagaaggc tataaggacg ctttggctgt
     3361 ttcagctgca ttgtctacgc agttgaaaat aagggatgag cttgtattga ttggttcgac
     3421 aggaatcatc ggagaaaagc ttcctgtcaa aaagataact ggtggcatcg gcaagctagt
     3481 caaaggatta aatgagtttg gtattgaaga tgcggaaacg gcgatgatga cgactgataa
     3541 atatcccaaa attgctatcc ggaaaggaat cgtcggagca aaagacatta ccatttgtgg
     3601 tatagccaag ggtgccggca tgattgaacc caatatggcc acattgctaa cttatgtgat
     3661 aaccgacgct ttaattgatt ccaaggctct aaatactgta ttccatcagg cgattgatgc
     3721 taccttcaac tccattagcg ttgacggatg tatgagtacc aatgatatgg cgattatttt
     3781 agctaacgga ctggccggca ataatccctt ggaaagagcg caagcacgtt tgcagcgttt
     3841 tcgtgacatg ttatgcgatg ttttaaagga actctgccag gcagttgtgc aagatgggga
     3901 aggggcgaca aaatttattt ccgtcatagt ggaaggcgct aaatcaaagt cggatgcgcg
     3961 gcgtgccgct tatgctattg ctaattctaa cttggtcaaa acggcgtttt ttggtgaaga
     4021 ccctaattgg ggaagaataa tcgctgccct tggttcgtcc ggaatacctc tggaaaagga
     4081 aaaagttagc ttatccattg gaaatatgac ggtatattcc caagattcac cggttaatat
     4141 taatttaaac aaattaaaag aaatatttca aaaagatcgg attgatgtgc gcgttggatt
     4201 aggagggggc gataaatcat attgcgttta tacatcggat ctttcttatg attacgtgaa
     4261 gatcaatgcc gagtactcga gttaaacggg ggaaagatta attttattgc cttaaaaccg
     4321 tgatttagct tgatcattta gattatttat gttaattgcc gccaaatttc acacatcctt
     4381 ggattgcccg gcatgttgct tttaattaag tgtgccggac aagttgaagg ggatggagga
     4441 aaaaaacaaa tgaggagaaa aatatgtcag caatttcaat gaaactttta ctggaagcgg
     4501 gtgtccattt cgggcatcag accaacaaat ggaaccccaa gatgaagcag tacatttttg
     4561 gggcgagaaa caacatttac attattgatc tacaacaaac cgtcaccatg tttcaaacag
     4621 cctataactt cgttgttgat accgtcagcc aaggcaaaga gattcttttt gtcggaacta
     4681 aaaagcaatc gcaggaatct attaaagaag aggctatacg ttgcggcatg ccgtacgtga
     4741 atcagcgctg gctcggtggt atgttgacta atttctttac aatcaaaaaa agcatcgatc
     4801 gtttaaacac attaaccatc atgttttctg acgaatccat taaggcattc cccaaaaaag
     4861 aaattacaaa attgcaaaaa gaaaaggaac aactagaaaa tgttttaggt gggattagaa
     4921 atatgaaatc tcatccggca gctgtgttta ttgttgatcc taaccgtgaa cacataacag
     4981 ttaatgaggc gaaaaaatta aaaattccta tagtagcgat agttgatact aattgcgatc
     5041 ctgacgatat cgattacgtt ataccaggaa atgatgatgc catcagggct attaaactat
     5101 ttgcctctaa atttgccgat gcggtgttgg agggaaagaa acgctttgaa gaaaagctaa
     5161 atgcggaatc aaataaagaa gcagggacgg tcgtggaaac caagtccgtg gaaaccgaca
     5221 ttccggaaag catagaaatg gaaggtaaga ctgcagattc ggaagaatat cgtgatgaat
     5281 cacatacata actagaaacg atcttgaatg tattttaagg agggaa
//
LOCUS       DASVIM010000203         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1531803, whole genome shotgun sequence.
ACCESSION   DASVIM010000203 DASVIM010000000
VERSION     DASVIM010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531803"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..923
                     /locus_tag="VF305_04090"
     CDS             168..923
                     /locus_tag="VF305_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416343.1"
                     /translation="MPSSWKLVSPEGPPVIIRGLERDGITPDLTTGAYYEYNLKRTFI
                     LLNHKGRQVFISISKQIDKSDVGKKGVILGNDDDWNYYYSGVPGSTKTGLGWIKSYIY
                     DFFSVGVYVESGASSSMVRSGVFQWIRAGWSGINFVQTAHIIKGMKRNAKNLKTILES
                     PNLPAPNQIVSTYQRLSALPQNDLIEKYTALQQARQSLAVLSGKSEKNEVKKQDSYAN
                     TLKEQIIGELMLEYFKITLGKSSLLGKKIVLGY"
     gene            1153..1812
                     /locus_tag="VF305_04095"
     CDS             1153..1812
                     /locus_tag="VF305_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7416344.1"
                     /translation="MGETILAIDDEKTNREFYFGLLKYKGYNVLKAENGEDALEILRN
                     ASVDLVLLDVMMPGISGFDVLKSIRENKKLQSLPVIIITALADKENRLKGLQLGADDF
                     IAKPFDVDELVVKIGSQIKLAFLRNQIFEKGKLVNIVDRLDEGIIITDSKFMPIVTNF
                     KAKNILGMKEEPENIITHFKKIFNKDIYPNQNSNYFIVKSQEVDKPGIFSLNVHLSRT
                     K"
     gene            1947..>2094
                     /locus_tag="VF305_04100"
     CDS             1947..>2094
                     /locus_tag="VF305_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7416345.1"
                     /translation="MSKAKLPEYDYDTVIIGGGPAGYTAGLYAARAGLKAMLVEGAAT
                     VSQIT"
BASE COUNT          675 a          407 c          429 g          583 t
ORIGIN      
        1 tccccaaact tcaggaagcc cctggagcct attatgaatt cgataccagg atcaacttct
       61 ccagtttttt gaagtattcc tatagtagcc aaattccatt agctctcacg agcccagctt
      121 ctctgcggta ttccctatgg accggcctcc caggggaagc acaaaaaatg cccagtagct
      181 ggaaattagt ttcgccagaa ggtccgcctg tcatcattcg cggattggaa cgcgacggca
      241 tcactccaga cctcactacc ggcgcctact atgaatacaa tctaaaaaga actttcattc
      301 ttctcaacca taagggccgg caggtgttta tttccatttc caaacagatt gataaatccg
      361 acgtcggtaa aaagggtgtc atcctgggga acgatgacga ttggaattac tattattcgg
      421 gtgtgcccgg ctcgaccaaa acaggcttgg gttggattaa atcctacatc tatgactttt
      481 tttcggtggg agtctatgtt gagtcaggcg cttcgtcatc catggtcaga agcggcgttt
      541 ttcaatggat tcgggctggc tggtccggga tcaattttgt ccaaacggcg catatcatca
      601 agggaatgaa acgaaatgcc aaaaatttaa aaacaatcct ggaatcgcca aacttgcccg
      661 cgccaaacca gatagtctcg acttatcaac ggctttcagc cttaccccaa aatgatttaa
      721 ttgaaaaata tactgccttg cagcaggctc gacagtccct ggccgttctg agcggtaaga
      781 gcgaaaaaaa tgaagtaaaa aaacaagact cctatgcgaa cacccttaaa gaacaaatca
      841 ttggagaatt gatgctggaa tatttcaaga ttaccctggg caagtcgtcc ttgttaggaa
      901 aaaaaatagt tttgggttat taattaatca gtttttcttt aagtcagtaa cttgggggac
      961 ccggtgcgtg ctctccgata atcccgaaaa aacaagcacc caaacacacc cctacattat
     1021 aaatatcaaa gtataacacc ctcatgtcgt tgttattatt ccattgacat gcaagtgagt
     1081 tcttcttata attgctccta acaaattgaa aaaacaaata tatactctta taagacccgg
     1141 aggcatttag gaatgggaga aactattctg gctatcgatg atgaaaaaac gaacagggaa
     1201 ttctattttg ggcttcttaa atataaaggg tataatgttt taaaagcaga aaatggggaa
     1261 gatgccctgg aaatattgag aaatgcgtca gttgatttag ttttgttgga tgttatgatg
     1321 ccgggaatat ccggttttga tgttttgaag agtatacgcg agaataagaa actgcagtct
     1381 ttacctgtaa ttattattac agccttggct gataaggaaa atcgccttaa aggcctgcaa
     1441 ttgggcgctg atgattttat cgcaaaacct ttcgatgttg atgaattagt cgttaaaata
     1501 ggctctcaaa ttaaactcgc ttttttaaga aatcaaatat ttgaaaaagg taaattagtg
     1561 aatatcgttg ataggcttga cgagggaata ataattacgg acagcaagtt tatgcctatt
     1621 gttactaatt ttaaagccaa aaatatttta ggaatgaaag aggaacctga aaatataata
     1681 acccatttta aaaaaatttt taataaagat atatatccta atcaaaacag taattatttt
     1741 attgtaaaaa gccaggaagt tgacaagcca ggcatatttt ctttaaatgt tcatctgagc
     1801 cgtacaaaat agaataaaaa aagagtttgt cagataggca attataagga tagtaatttc
     1861 aatcttctaa caccttcact ttagacttta caaaatcatc tcttcctgat tatagtgaga
     1921 aaagaacgtc tagggagaca ctttctgtga gcaaagcaaa attgcctgaa tacgattacg
     1981 atacagttat tattgggggc ggaccggcag gctatactgc cggcttgtat gccgcccgag
     2041 ccgggcttaa agcaatgctc gtggaaggcg cggcaacggt aagtcaaata acca
//
LOCUS       DASVIM010000204         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_590852, whole genome shotgun sequence.
ACCESSION   DASVIM010000204 DASVIM010000000
VERSION     DASVIM010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590852"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VF305_04105"
     CDS             complement(<1..1392)
                     /locus_tag="VF305_04105"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX7416346.1"
                     /translation="MKQRIKKIFQITPLKITLFVILLALVLFFFDFRFLRFMELKALD
                     LRMASRGALSPGGETVIAVVDNESIKEIGRWPWSRATLAKLVDKLKAAGAKAVGFDMV
                     FSEPDYNSSLRSIDEISADMKKQGINGNVISILNKKRENADTDMVLAKSIARAQNVTL
                     GYFVYIGNKDAFKLSGKDISGRANRIKNSRYQIINSSEQDIDDSRIENVYAPEPNIKI
                     ISDAARNSGYFNTMPDSDGSMRWSPLVLRFQNNYYSSLAISVLMQYLDWPMLSLNMES
                     YGVEGIKLGNINIPTDDRGRLLINYPGPAKTFPHYSINDILKGKIPAEKLNNKIVLVG
                     VTATAVYDLRVTPFGNVYPGVEIHASVVDNILHQNFLKYSSLIRFIDIIVIIAFGLIM
                     SLIIVRLRAVKGLIAALIIIAAFVAANIFMFSRYNVWLNIVYPVLTMIVIYFGITIYR
                     YIKEEREKKKIRGA"
     gene            1686..1970
                     /locus_tag="VF305_04110"
     CDS             1686..1970
                     /locus_tag="VF305_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416347.1"
                     /translation="MSKVKTGDVLSCEVCGLTVVIDEECGCATAGVICCEEPMLNKGP
                     AAPKKKARAATAKKKKAITKKAKTAKKKKVAKKKPAKKPPAKKKATKKRK"
     gene            2134..>2470
                     /locus_tag="VF305_04115"
     CDS             2134..>2470
                     /locus_tag="VF305_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX7416348.1"
                     /translation="MCGRFVLISNLGQIAEEFNLTDMYSSVPFGDIHPGKTAACVIAE
                     DKKNRLLILPWGFSPPWSKQATKSGLLINARAETLAEKPTFREAFQKRRCLIAADGFY
                     EWSKEKNQFY"
BASE COUNT          774 a          505 c          514 g          677 t
ORIGIN      
        1 cgcgcctcga attttctttt tttcccgttc ttcctttatg taacggtaaa tcgtgatgcc
       61 aaaataaatg acaatcatcg tcaaaacagg ataaacaatg ttcagccaaa cattgtacct
      121 tgaaaacatg aagatatttg ccgccacgaa tgccgcaatg ataataagcg cagcaatcaa
      181 tcctttaacc gcccgcaagc ggacaatgat taggctcatt atcagaccaa aggcaataat
      241 aacgatgatg tcaataaatc ttatcaggga ggaatatttt aaaaaattct gatgcaaaat
      301 attatccacg acgctggcat gaatttccac cccgggatag acgttgccaa acggggttac
      361 gcgtaaatca taaacggcag tagccgtgac acccaccagc acaattttgt tgttaagttt
      421 ttcggccggt atttttcctt taagtatgtc attgatcgag taatgcggaa aagtttttgc
      481 cggccccggg taattgatca aaagccttcc ccgatcatca gtgggaatat tgatattgcc
      541 gagtttaatg ccttctacgc cataactttc catatttagc gacaacatcg gccagtccaa
      601 atattgcata agcactgaaa tggccagtga agaataataa ttattctgga atctaagaac
      661 taaaggcgac cagcgcatag agccgtcaga atcgggcatg gtattgaaat aaccgctgtt
      721 ccgggcagca tcagaaataa ttttaatatt gggctcgggc gcataaacat tttctatgcg
      781 cgagtcatcg atatcttgtt ccgatgagtt gattatctga tagcgcgaat ttttaattcg
      841 attggctctg ccggaaatat ctttccccga taatttaaaa gcgtctttgt ttcctatata
      901 gacaaagtat ccaagggtga cattttgagc gcgggcaatt gatttggcca ataccatatc
      961 tgtgtccgca ttttcgcgct tcttgtttaa aatggaaatt acgttgccgt taatgccttg
     1021 ctttttcata tcggcagata tttcgtctat cgatctgagg ctggagttgt aatcaggctc
     1081 agaaaacacc atatcgaacc caaccgcctt tgctccggca gcttttaatt tatcgaccaa
     1141 tttagcaaga gtagcccggg accagggcca acgtcctatc tctttaatgc tctcgttatc
     1201 tacaactgcg atcactgttt cgccccctgg agacagggct ccgcgcgagg ccatgcgcaa
     1261 atccagagct ttgagttcca taaaacgaag gaagcgaaaa tcaaaaaaaa agagaacgag
     1321 tgccagcaaa atcacaaaga gcgttatttt taaaggcgtg atctggaaaa ttttcttgat
     1381 tctttgcttc atggcttgcc cttccgttta actccaaagc gcacttaaac gaatatcttt
     1441 tacgattatt aacggagatt gtttctttcc agggtgtaaa attttatctc catcagaaca
     1501 tgcttttcac gagataatac caaaccgtcg atgaaagtca agctgtcagc tgtctatgtt
     1561 tttcaaaaaa atcggccgga aacgttcaat ctatgcttga aataattatt tttttgatta
     1621 gaataaaaca aataatgctg gtagggtaaa tatctaaacc gctcaacaac gaggaggaaa
     1681 ggcaaatgtc gaaagtaaaa actggagatg ttctgagttg tgaagtatgt ggacttaccg
     1741 ttgtgataga cgaagaatgc ggatgcgcta cggccggagt tatttgctgt gaagaaccga
     1801 tgctcaataa agggccggcc gcgccgaaaa agaaagcgcg cgctgcaaca gcgaagaaaa
     1861 aaaaggccat aacgaaaaaa gcaaaaactg caaagaaaaa gaaagtggca aaaaagaaac
     1921 ccgcaaaaaa acctccggca aaaaagaagg caactaagaa aagaaaataa ttagcccgtg
     1981 cagtgtcaat tctctgtttt ttcttataga gccccttagc ggggctttat aaggtgaatt
     2041 tcttacgaaa gcggtaacta ttacattgat atttccggca ggcagtcctt tatatttttt
     2101 ctaaaacaaa tatttctatc attgaggaaa attatgtgtg gaagatttgt tttaatcagt
     2161 aatttggggc aaattgctga ggaatttaat ttaaccgata tgtattcttc tgtgcctttc
     2221 ggtgatattc accccggcaa gacagccgcc tgcgtaattg ccgaagataa aaagaatcgt
     2281 ttgctgattt tgccctgggg attttcgccg ccatggtcaa agcaggcaac aaaatccgga
     2341 ttgctgatca atgcccgcgc agaaacttta gctgaaaaac caaccttcag agaagctttt
     2401 caaaagagaa ggtgcctgat agctgccgat ggattttatg aatggtccaa agaaaaaaac
     2461 caattttatt
//
LOCUS       DASVIM010000205         3437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_507769, whole genome shotgun sequence.
ACCESSION   DASVIM010000205 DASVIM010000000
VERSION     DASVIM010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507769"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VF305_04120"
     CDS             complement(<1..909)
                     /locus_tag="VF305_04120"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416349.1"
                     /translation="MYVNVALNIPADKLFTYAVPTNLQQEAQIGKRVFVPFGRRKRTG
                     FIVAINVPCSLTNIKSVMEILDDEALFGNADLDFYQWIATYFMYPLGKTLAELIPAGP
                     EKKDFLWITPLDLPADISVSPAQEKLLTLLQQYPQGISLHALTEESGLKNISAIAQNL
                     HLSGLLRIEEKNKKHLAVRSEKIVSLVDEKIAGTKLTGRQELVVEYLQKHGSMAIGNL
                     IKKANTSSSVINNLHLKGIIKFTASEVIRRASLQPTISRAEEKITLNNEQKLALQEIS
                     QNLGKNIFTPVLLHGVTGSGKTEVYLN"
     gene            complement(1018..1398)
                     /gene="dksA"
                     /locus_tag="VF305_04125"
     CDS             complement(1018..1398)
                     /gene="dksA"
                     /locus_tag="VF305_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX7416350.1"
                     /translation="MEKQTTLKPEKMEEFRLMLIQKINELLSEAGKTVSEMTTGKENF
                     PDPNDRASLESDRNFELRIRDRERKLIMKMQEAIKRIDDGIFGICEVCGGPISEKRLT
                     ARPVTTLCIDCKTKQEKIEKLKGE"
     gene            complement(1548..2033)
                     /gene="rfaE2"
                     /locus_tag="VF305_04130"
     CDS             complement(1548..2033)
                     /gene="rfaE2"
                     /locus_tag="VF305_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX7416351.1"
                     /translation="MNKILERNVLKNKLEELRREGKKIAFTNGCFDILHVGHIRYLRE
                     AKKTADVLVLALNSDSSVRAIKGEKRPLMSEEERAEILAALEFIDFVTIFQELTPLEL
                     INYLKPDILIKGGDWPEEKVIGREEIKKWGGRVAIIPEVEGKSTTNIVEKIKKLYCSN
                     K"
     gene            complement(2058..2792)
                     /locus_tag="VF305_04135"
     CDS             complement(2058..2792)
                     /locus_tag="VF305_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX7416352.1"
                     /translation="MILIEALNNISDVIFPPQCLGCAEILHPQSGQLFCPACKEKIKF
                     ITGNLCPICGRAYFDSPAESHLCGNCLGNKPYFSCARAVASYETLILEAIHQFKYGRN
                     LSIGSALASFLADFSFPDFEFQVSSLIVPVPLHIKKLRERGFNQSLILANALGEKQRI
                     DVNFSLLKRRKFTLTQTGLNRTEREKNVKGAFAVSDKKKVAGKNVILIDDVYTTGATI
                     NECAKTLIKAGAKKVAVLTLARVLQS"
     gene            complement(2789..3184)
                     /gene="rsfS"
                     /locus_tag="VF305_04140"
     CDS             complement(2789..3184)
                     /gene="rsfS"
                     /locus_tag="VF305_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX7416353.1"
                     /translation="MPAKKNESKKRLLLCINATLQKKAKDIVVLNLKEISSFTDYMLI
                     CSGTSERQVQAISAAIREFLKKEGIKPLGIEGEANGQWILLDYDDVVISIFHEPLRAF
                     YAIENLWDAPRMNIDGNATEIKRLNKGLT"
BASE COUNT          943 a          709 c          690 g         1095 t
ORIGIN      
        1 gtttaaataa acttctgttt taccgctgcc ggtgacgcca tgaagtaaaa ccggtgtaaa
       61 aatattcttt cccagattct gagaaatttc ctggagggcc agtttttgct cattgttaag
      121 agtgatcttt tcttcggcgc ggctgattgt tggctgaagg gaagctctgc ggattacttc
      181 gctggcggta aatttgatga ttcctttaag atgaagatta ttgatcacgg agctggaggt
      241 attggccttt ttgatcaagt tgccgatagc catggagccg tgtttttgca gatattcgac
      301 cactagttcc tgtctgccgg ttagttttgt tccggcaatt ttttcatcca caagagaaac
      361 gattttttca ctgcgcacag ccagatgttt cttgttcttt tcttctatcc gcaaaagccc
      421 cgacagatga agattttgcg cgatagcaga aatgtttttc aagccgcttt cttcggtaag
      481 agcgtgcaaa gaaataccct gcggatattg ctgcaataat gtaagaagtt tttcctgtgc
      541 tggcgaaacc gaaatatcgg caggcagatc caggggcgtg atccagagaa aatctttttt
      601 ttcgggacct gcgggaatca gctcggctaa agttttaccc agcggataca taaaataagt
      661 agcaatccac tgataaaaat caagatcagc attgccaaaa agtgcctcgt catcaagtat
      721 ttccataacc gattttatat tggtcaggct acagggaaca ttaatggcga caatgaaacc
      781 ggtgcgtttt ctgcggccaa aggggacaaa gacccgtttg ccgatttggg cttcctgctg
      841 caggtttgtc gggactgcat aagtgaatag tttatctgcc ggaatgttga gggcgacatt
      901 tacatacata tgaccttgtt atcatcttta gttttaaaat ggaattattt cccggtagtt
      961 taagccaaaa aaaacgcccc aataccggag cgtttttagg aatttatgat tttgatttta
     1021 ttcacctttg agcttctcga ttttttcctg ctttgttttg caatcgatgc aaagagtggt
     1081 aacgggtctg gctgtcaatc ttttttcgga aatcggccca ccgcaaactt cgcagatgcc
     1141 aaaaataccg tcatcaattc ttttgattgc ttcctgcatt ttcatgatga gcttgcgttc
     1201 tctatccctg atgcgtaatt caaaattccg gtctgattcc agagacgctc tgtcattggg
     1261 atcagggaag ttttctttgc cggtggtcat ttcggaaaca gtttttccag cttcgctcag
     1321 caactcattg attttttgaa tgagcattaa tctaaactct tccattttct ccggcttcaa
     1381 agtcgtttgt ttttccaata ccgctactcc ttgtgtatgt gatgttgagt ttgtcgctct
     1441 tattacctaa tggtgtaatg tttgtaaaga aaaaaaatta caccaacaat atctaaagat
     1501 catattctcc aagcacaaag ggatttaagc aaaattaact aagtgattta tttattagaa
     1561 caataaagct tttttatctt ttccacgatg tttgttgttg atttgccttc aacttcgggg
     1621 attattgcca cccggcctcc ccattttttt atttcttccc gcccaatgac cttttcttcc
     1681 ggccagtcgc cccctttgat aagaatgtcc ggcttcagat aattaattag ttccagcggt
     1741 gttaactcct ggaatatagt aacaaaatca ataaattcaa gtgcagcaag gatttccgct
     1801 ctttcttcct cgctcatcag cggccttttt tctcctttga ttgcccgaac ggaagaatcg
     1861 ctgttgagag ctaagaccaa aacatcagca gttttcttcg cttcgcgtaa ataacggata
     1921 tgaccgacgt gtaaaatatc aaagcagcca ttggtaaaag cgatcttttt gccttccctg
     1981 cgcagttctt ccaatttgtt tttgagcaca tttctttcta aaattttgtt cattcccacc
     2041 tcttgttgtt gaaccaatta actttgaaga actctggcta aagttaaaac agcaactttt
     2101 tttgctccgg ctttaattaa tgttttggca cactcattga tagtggctcc ggtagtgtaa
     2161 acatcatcaa tgaggataac atttttaccg gcaacttttt tcttgtcact tactgcaaag
     2221 gctcccttta catttttttc tctttcggtt ctgtttaaac cggtttgagt taaagtaaat
     2281 ttacgccgtt ttagcaagga aaaatttacg tcaatccgct gtttttcccc cagggcattt
     2341 gccaaaatca gagattgatt aaagccgcgc tctcgcaact tttttatatg caggggcacg
     2401 ggaacgatga gagaggaaac ttgaaattca aaatcaggaa atgaaaaatc tgccaggaaa
     2461 gaggccagtg cggagccgat ggatagattg cggccatatt tgaactgatg gattgcctcc
     2521 aaaataagag tttcataact agcgacggcg cgggcacaag aaaagtaggg cttatttccc
     2581 aggcaatttc cgcataaatg gctttcggcc ggactgtcga aataagctct gccgcaaata
     2641 ggacaaagat tgccggttat aaatttaatt ttttccttgc aggccggaca aaacaattgt
     2701 ccgctctgcg gatgcaaaat ttcagcacag cccaaacact ggggcggaaa aataacatcg
     2761 ctgatattat ttaaggcttc aattaatatc atgtcaaccc cttatttaac cgctttattt
     2821 ctgtggcatt tccgtcgata ttcatacgag gggcatccca taaattttca atagcataaa
     2881 aagctctaag aggttcatga aagatagaaa tcacgacgtc atcgtaatca agcaaaatcc
     2941 actgaccatt ggcctcgcct tcgataccca gaggtttaat tccctctttc tttaaaaatt
     3001 cccgaatcgc ggcggagata gcctgaactt gccgttcgga tgtcccgctg caaataagca
     3061 tataatctgt aaaagaagaa atttctttta gattcaaaac aacaatatct tttgcttttt
     3121 tctgtaaagt tgcgttgatg cacaaaagaa gacgcttctt ggattcgttt ttttttgccg
     3181 gcaaataaac ctcccattaa acattggatt tttacaacat atgccacaaa taataaaagt
     3241 tgacaatgtt gcatttaatc ttgattgccg gccctggata atgccggcat attcggcaca
     3301 atgtgatgtt cttttttagc tggtgagaaa gtggtaattc aaataatcaa taataagaag
     3361 tgtctgttgt agaaaaaaag tggctatgat aagtgaattt acatcatcaa tgcggttaag
     3421 tcatgtaaag ggttttt
//
LOCUS       DASVIM010000206         5810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_762663, whole genome shotgun sequence.
ACCESSION   DASVIM010000206 DASVIM010000000
VERSION     DASVIM010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762663"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..243)
                     /locus_tag="VF305_04145"
     CDS             complement(64..243)
                     /locus_tag="VF305_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416354.1"
                     /translation="MEKLPGGKVPERADFLAYLDDQEIRITNWHKAVELSAPLGEDFI
                     ELVAGGKIKDVVEKL"
     gene            complement(286..1131)
                     /locus_tag="VF305_04150"
     CDS             complement(286..1131)
                     /locus_tag="VF305_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416355.1"
                     /translation="MNRIRIKAGKRAYKIIKDGGFKFDSVSTYFGPAVGPRWLLASGF
                     DLTLLKGEFLGRSRAVNLVGSSAGAWRFAAWLQPEASQCYQKLLDAYINTRYFRKDNP
                     SSVLEKFTGLVNEYLEDDALPFALTNKKYRLTIITARARGLLASEIQWLQKLGLAACY
                     LFNRISRNNIYHFAERIVFHNSSMPPSFCLQPQFRGRYVRINEINFKYAVMASGAIPL
                     VVAGVHNIYGAPRGIYRDGGLIDYHLTHQFAAKENETVLFFHHQERIIPGWLDKNLIH
                     RVPDE"
     gene            1326..2582
                     /locus_tag="VF305_04155"
     CDS             1326..2582
                     /locus_tag="VF305_04155"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX7416356.1"
                     /translation="MDIKDSIKQIAQDAVLAARTLSRVTSAAKNNALLQMADELARNC
                     DFLLAENAKDVSKAEQAGLSPALIDRLTLEKATIESMAQGLREVAALPDPVGKITSMW
                     RRPNGLLVGRMRIPLGVIGIIYESRPNVTVDASALCLKSGNAVILRGGSEAINSNLAI
                     ASILQEVLRKSSLPAAAIQVIPLTDREAVSAMLQLDEYIDLIIPRGGEELIRAVVAQS
                     KIPVIKHYKGVCHIFVDAQADMEMAVNICFNAKVQRPGVCNAVETLLVHKDIAREFLP
                     QVADKLQKAGVVLRGCAKTKSILKDIEAATEDDWYAEYLDLILAVRVVENIDEAIAHI
                     EKYGSLHTESIITNDYNNSQRFLNEVNSSTVLVNASTRFSDGFELGLGAEIGISTTKL
                     HAFGPMGLEELTTTKFIIYGNGQVRT"
     gene            2579..3229
                     /gene="nadD"
                     /locus_tag="VF305_04160"
     CDS             2579..3229
                     /gene="nadD"
                     /locus_tag="VF305_04160"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX7416357.1"
                     /translation="MKWGLLGGTFDPIHFGHLRGAEEMLGIFNLNRIIFIPSSRPPHK
                     LEAEITSFYHREQMIRLAIEGNVNFSFSEVENLRAGKSYSVETVEYILNKYMEDLELY
                     FIVGQDAFQAVTTWKDWEKLLLLCNFAVMTRPGYENMKLDGILPKDFAARFTYDEKID
                     GFRGPTGHTIYSRHVSFLDISSSRMREMIKADKSIRYLTPDKVRQYIFKNSLYKKL"
     gene            3336..4181
                     /locus_tag="VF305_04165"
     CDS             3336..4181
                     /locus_tag="VF305_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7416358.1"
                     /translation="MAKIFKKLFILNLVIALFIFCSTPSMAENAAPVENSSAIKLGIT
                     YYHFDYKEDLVMPAKSTEYGWLPGVYLDYTFKKKSSIYTKVFLSYAGDNITYDGSNTA
                     GQPVKYTYETARMLKFEANIGYAIPIGKDFLLIPYLGYGYQYWERGEAQYLAAFDTFS
                     YQEIYKWHYIPVGIKADYNITKKLNIAASAAANFMFYGQMKAYLSEIPVGYPDMDFTL
                     GNRVGFYAEIPVTYKFTNNIGISVTPWYEYSSFGESDVNVYGFYEPSSKTNKYGVNVG
                     VLFSF"
     gene            4891..5271
                     /locus_tag="VF305_04170"
     CDS             4891..5271
                     /locus_tag="VF305_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7416359.1"
                     /translation="MPKKILIADDDQNTVSFLVSLLKANKYEVVVAFDGMQTIAVAHK
                     EKPDLILLDIMMPVRSGINIFENLKMSALTATTPVIFITGVANPEIKQKALEMGAADY
                     IVKPFETEYLLSKVRKALGEKNRV"
     gene            complement(5471..>5810)
                     /locus_tag="VF305_04175"
     CDS             complement(5471..>5810)
                     /locus_tag="VF305_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX7416360.1"
                     /translation="GIAPAYAVPLAMKRAGLKLSAMDVMECNEAFAVQNLAVVKELEK
                     QTGEKVNMDKWNPNGGAIAFGHPNGASGARLCIFTMKELIRKGGKYGVFSSCCGGGLG
                     VATVIENLQK"
BASE COUNT         1753 a         1221 c         1223 g         1613 t
ORIGIN      
        1 gtgagcttta gatttatcca aaaaaatcat ggccaggcaa aaatgtattt agagataatt
       61 cgcttacagt ttttctacga catccttaat cttgccgccc gccaccagtt cgataaagtc
      121 ttcgcccagc ggcgcagata actccacagc tttgtgccaa tttgtaatcc ttatttcctg
      181 atcatcaagg taggcaagaa aatcagcgcg ctcgggaact tttccacccg gaagtttttc
      241 cacaaaactt tgtgtgggga ataccatcaa gacattactt aatgtttact catcgggtac
      301 acggtgtatc agatttttat cgagccagcc cggaatgatc cgttcctgat gatgaaagaa
      361 aagtactgtt tcgttttctt tagcggcaaa ctggtgtgta agatgataat cgatgagacc
      421 gccatcgcgg taaatgccgc gcggagcgcc ataaatgtta tgcacaccgg caacgacaag
      481 agggatagcg cctgaagcca tgaccgcata cttgaaattg atttcattga ttcttacgta
      541 gcggccgcgg aactgcggct gtaaacaaaa ggacggcggc atggaactat tatgaaatac
      601 gattctctcc gcaaaatggt agatattatt acggctgatg cgattaaata aatagcaagc
      661 ggccaaacct aatttctgta accactgaat ttctgaagcg agaagaccgc gtgcccgggc
      721 agtaattatg gttaaacggt attttttatt cgtcaaagca aagggcagcg cgtcgtcctc
      781 taaatattca ttgacaagac cggtaaattt ctccaacacg ctggaaggat tatcttttcg
      841 gaagtatctt gtatttatat aggcatctaa aagtttttgg taacattggg aagcttccgg
      901 ttgtaaccag gcggcaaagc gccatgcacc cgcggaggaa ccaactaaat tcacagccct
      961 ggaacggcct aaaaattcac ctttcaagag agttaaatca aagccgctgg caagcagcca
     1021 gcgtggccct actgccggcc caaaataagt tgaaacagaa tcaaatttaa atccgccatc
     1081 tttgataatt ttgtaagctc gcttaccagc tttaattctt atcctattca tatttatttt
     1141 ctatcccggc aaaggtcttg acacaaaaat tattgtcctg taaattaaga caactattga
     1201 ttattggtaa atttcttata tcctgtttat aaagttaccc aaccctcatt aagtgaggcg
     1261 gcttttatca gtaattagaa aaaatgcaag taaatttatt ttttagactt ttccaaggat
     1321 aagtcatgga tataaaagat tcaataaaac aaatcgccca agacgcggta ctggcagcgc
     1381 gaactctttc tcgcgtaaca tcagcagcga aaaacaatgc cctcttgcaa atggcggatg
     1441 aacttgcccg caattgtgat tttcttctgg cggaaaacgc caaggacgtt tccaaggctg
     1501 aacaagcagg cctttcccct gccctgattg accgccttac tctggaaaaa gccacaattg
     1561 aatcaatggc tcaaggacta cgggaagtag ccgcgcttcc cgatcctgta ggcaagataa
     1621 cttccatgtg gcgccgtccc aacggtcttt tggtaggcag gatgcgtatt cctttaggag
     1681 ttatcggcat catttatgaa tcgcgcccca atgttacggt tgatgcctcc gccctttgcc
     1741 tcaaatcggg aaacgcggta attttacgcg gcggttccga agcaattaat tccaatttag
     1801 ccatcgcctc catcctgcaa gaagttttgc gcaaaagctc cttgcccgca gcagcaattc
     1861 aagtaattcc cctgactgac cgtgaagccg tatcggcgat gctacagttg gatgaatata
     1921 ttgatttgat aattcctcgc ggcggggaag aattgattcg cgccgtggtt gcccaatcca
     1981 aaatacccgt aatcaaacac tacaaggggg tctgtcatat ctttgttgac gcgcaggccg
     2041 atatggagat ggctgtaaat atttgcttca acgccaaagt tcagcgcccg ggcgtttgca
     2101 atgccgtgga aaccttactg gtacacaaag acatcgctcg tgagtttttg ccacaggtag
     2161 ccgataaatt gcaaaaagca ggtgtcgtcc tcagaggatg cgcaaaaaca aaatcaatat
     2221 taaaggatat tgaagctgca accgaggatg actggtatgc agaatatctc gatttaattc
     2281 tggccgtaag agttgtggag aatattgatg aagccatcgc tcatattgag aagtatggtt
     2341 ctttgcatac ggagtcaata ataacgaacg actataacaa ttctcaacgc tttttaaacg
     2401 aggtcaattc ttcaaccgtg ctggttaatg cctccactcg tttcagtgat ggttttgagt
     2461 tggggttagg cgcggaaatt ggcatttcga caacaaagct tcatgccttt ggaccaatgg
     2521 ggctcgaaga attaacaaca acaaaattta taatttacgg caacgggcag gtgcgaacat
     2581 gaaatgggga cttttgggag gaacttttga tccgattcat ttcggacatt tacggggcgc
     2641 ggaagaaatg ctgggaatct ttaatttaaa ccgcatcatt tttatcccat catcgcggcc
     2701 tccgcataag ttagaagccg aaattacttc tttttaccat cgcgagcaaa tgattcgtct
     2761 ggcaattgaa ggcaatgtta atttttcttt ttccgaagtg gaaaatctac gcgccggcaa
     2821 atcttattcc gtggaaacgg tggaatatat tctcaacaaa tatatggaag atttggagct
     2881 atactttatt gttgggcaag atgcttttca agcggttacg acatggaaag attgggagaa
     2941 acttctgctg ttgtgcaatt ttgccgtaat gacacgtccc ggatatgaaa atatgaaatt
     3001 ggacggcatc ttaccgaaag actttgccgc acgatttact tacgatgaaa aaattgacgg
     3061 atttagaggt ccgaccggtc acaccatata ttcccgtcat gtaagctttt tggacatttc
     3121 ttcatcccgg atgagagaaa tgatcaaagc agacaaatct ataagatatc ttacgccgga
     3181 taaagttcgc caatacatct tcaaaaattc gctctacaaa aaattatgag gaaataattt
     3241 gacaagccta aggttgtatg ttttttaata cgtaagtgta cctgaaagca aattacctca
     3301 tatagatggt aaaaatgaaa aaggatggaa atgttatggc aaaaatattt aaaaaacttt
     3361 ttattttaaa tttagtaata gcacttttta ttttttgcag taccccgtca atggcggaaa
     3421 acgcagcacc ggttgaaaat tcgtcagcca tcaagctggg tataacttat tatcattttg
     3481 actataaaga agatttagtc atgcccgcca aaagtacgga atacggatgg ttgccgggcg
     3541 tttatttaga ttatactttc aaaaagaagt cctcaatata tacaaaggtc tttttaagtt
     3601 atgcgggtga taatataacc tatgatggat caaatacggc cggtcagccg gtaaaatata
     3661 cgtatgaaac cgccaggatg ctcaaatttg aagcaaatat tggatacgcc atacccatag
     3721 gcaaggattt tctgttaatt ccttatctgg gctacggtta ccagtactgg gagcgaggtg
     3781 aagcccagta ccttgcagcg ttcgacactt tcagttatca agaaatatat aaatggcact
     3841 acattcccgt tggaatcaaa gccgattaca atataactaa aaaattgaat atagccgcct
     3901 ctgccgcggc caatttcatg ttttacggcc aaatgaaagc ttacttatca gaaataccag
     3961 tcggctatcc cgacatggat ttcactttag gcaaccgcgt ggggttctac gcggaaattc
     4021 ccgtgaccta taaattcacc aataatattg gaatttcggt aacaccatgg tacgaataca
     4081 gttcatttgg agagagtgat gtaaatgtgt atggctttta tgagccgtca agcaaaacca
     4141 ataagtacgg cgttaatgta ggagttctct tttcattcta ggatattaca gctttcttgt
     4201 atggcaagta atttcccgga taggcggtat gactaccgaa ttaattaagt aataatcttt
     4261 aaaagctaca aaaaaaggcc atggagcaaa tcccatggcc tttgcagata cagttgaatt
     4321 tttaagccat ttctccaccc ccgcggaata caacaaccat catcttctac aacgtccaga
     4381 ctcccagcga tcctttgaag cttttcctga aaattgccaa gaaactctat acccccgtct
     4441 catctgctac cttctactga tgacttttca accggacatt ttatgtgctt ctgacaatta
     4501 ttttttctta tgttgacggg ggtttttatt ttgttataat cttaataatg aaagattaaa
     4561 tctttaaggt aagccctcgt aggaaaattt atctatgacc taagcaataa acattaggac
     4621 atccccaagc tgccagagct gctgtaaact atcctaaccc aaaaggctac ctttgataac
     4681 tattaggtaa aacatgattt ttctactatg ggcagacgta tcatgctttt gaatgcccgg
     4741 caaatttgag aaaagaaagg gataggggct ctagcataat tagctatcct gacaatggca
     4801 tgacgactga agattttagc cttaagataa cgaagatcgc aaatgaagct atgaaagatt
     4861 aattattgaa ataggaagga gcaacaaatt atgccgaaaa aaatactgat tgcagatgat
     4921 gatcaaaata ctgtatcatt tcttgtttct ttactgaagg ctaataagta tgaggttgtt
     4981 gtggcttttg atggaatgca gaccatagca gtagcccata aagaaaaacc cgatttaatt
     5041 cttttagata tcatgatgcc cgtacgtagc ggcataaata tttttgagaa tttaaaaatg
     5101 tccgccttga ccgccacaac ccctgtaatt tttattaccg gtgtcgctaa tccagagata
     5161 aagcagaaag cgctggagat gggtgcggca gattacattg taaagccgtt cgaaactgaa
     5221 tatctgctaa gtaaagtaag aaaagctctg ggcgagaaga atcgcgtgta attccgggga
     5281 tacttaatta ttgacgggag aagagatcgt cacgcagatg aatcgcgata gcggcaggtg
     5341 catactttac cgttgtagaa attaacatct ccgtggctga caccgccacg gagatattgg
     5401 aaacacacac attttgtaac gctaatgttt taataagcgc aaaaataatt aataactcca
     5461 ttgcaatttt ttatttctgt aaattctcaa taaccgtcgc tacacccaag ccaccaccgc
     5521 agcaagaact aaaaacacca tacttaccgc ccttgcggat gagttctttc atggtgaaga
     5581 tgcaaaggcg ggctcccgag gctccgttgg gatgaccaaa ggcaatggct ccgccgttgg
     5641 gattccattt gtccatatta accttttccc cggtctgttt ttcgagttct ttgacaaccg
     5701 ccaggttctg cacggcaaag gcctcattgc attccataac gtccatggcg gaaagtttca
     5761 agccggctcg cttcatggcc agaggaacgg cataagcagg agcaatcccc
//
LOCUS       DASVIM010000207         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1191511, whole genome shotgun sequence.
ACCESSION   DASVIM010000207 DASVIM010000000
VERSION     DASVIM010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191511"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2063)
                     /gene="leuS"
                     /locus_tag="VF305_04180"
     CDS             complement(<1..2063)
                     /gene="leuS"
                     /locus_tag="VF305_04180"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX7416361.1"
                     /translation="MNIKYDPRQIEEKWQTKWDKEKTFKVTEDPQREKYYLLEMFPYP
                     SGKIHIGHVRNYTIGDVVARYKRMKGYNVLHPMGWDAFGLPAENAAIEHKIHPSQWTN
                     DNIAHMKMQLKRMGFSYDWDREISTCEPQYYRWEQLFFLWMYEKGLAYKKRSTVNYCS
                     KCETVLANEQVEGGLCWRCGTEVNEKTLDQWFFQITAYIEELLDYCDKLPGWPEKVLT
                     MQKNWIGKSYGCEVDFPLIGSEGAIKIFTTRQDTLFGATFMLIAAELPLVMELSKGKA
                     IEKDVQQFVEKVKKQDRLMRTSEYYEKEGIFLEAYCENPLTGKKMPVYATNFVLADYG
                     TGCVMAVPTHDQRDFEFAKKFNLPLIVVIQPKGKKLDAEKMTEAYTEEGILVNSGKFD
                     GMENLQALESIADYLESIGRGKRMIQYRLRDWGISRQRYWGAPIPMINCKKCGIVPVK
                     ESDLPIVLPRDVVLSGAGGSPLAALKEFVQTSCPKCGGTAERETDTMDTFVESSWYFD
                     RFCSPDMDKKPGLDRKKLDYWMPVDQYIGGVEHAIMHLLYARFYTKMLRDFGVIGVDE
                     PFVNLLTQGMVCKETTKCPEHGYLFPEQAAEEKCHICQKQVTIGKTEKMSKSLKNVID
                     PDYLIETYGAYTARIFCLFAAPPEKDLEWSDQGVEGSFRFLNRLWRIFAEYLDDIKKV
                     KPLSG"
     gene            complement(2169..>2609)
                     /locus_tag="VF305_04185"
     CDS             complement(2169..>2609)
                     /locus_tag="VF305_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7416362.1"
                     /translation="IEPCLIHRDLQSQNIMLKNGKPVLIDFQGMRLGNFFYDLGSLIC
                     DPYVVFTNEERKELISFYYQLAKPTFGEKEFEIYFREASAQRLMQALGAYGFLGLKKN
                     KTEFLRHVENGVKNLIFVTSHLTTLSTLHELIKKCADKMSLQML"
BASE COUNT          655 a          615 c          574 g          765 t
ORIGIN      
        1 ctgccggaaa gcggctttac tttcttgatg tcgtctaaat attccgcaaa tatgcgccac
       61 aggcggttca agaaacggaa tgatccttcc actccctgat cactccattc caaatccttt
      121 tccggaggag ccgcaaaaag gcaaaaaatg cgggctgtat aagcgccgta agtttcgata
      181 agatagtccg ggtcgataac gttcttcagc gatttggaca tcttttccgt tttgccgata
      241 gttacttgtt tttggcagat gtggcacttt tcttccgctg cctgttcggg gaaaaggtat
      301 ccatgttccg ggcatttggt tgtttctttg caaaccattc cctgggtgag caggtttacg
      361 aatggttcgt ccacaccgat aacaccaaaa tcgcgcagca tcttggtata aaaacgggcg
      421 tagagcaaat gcatgatggc gtgttcaacg ccaccgatgt actgatcaac cggcatccaa
      481 taatccagct ttttacggtc cagaccgggt tttttatcca tatccggact gcagaagcgg
      541 tcgaaatacc aggaagattc cacaaaggta tccatcgtat ccgtttctct ttcggccgtg
      601 ccgccgcatt tcggacaaga agtttgaaca aactctttta gagccgccag cggtgatccg
      661 cctgcaccgc tcaatacaac atcacgcggc agaactatgg gcaaatctga ttctttaacc
      721 ggaacgatgc cgcatttttt acagttaatc atgggaatgg gcgcgcccca gtagcgctga
      781 cgcgaaattc cccaatcgcg caggcggtat tggatcattc gtttgccgcg gccgatcgac
      841 tctaaataat cggcaatgga ttccagagcc tggagatttt ccatgccgtc aaatttaccg
      901 gaattgacta aaattccttc ctccgtgtaa gcttcggtca ttttttcagc gtcgagtttc
      961 ttgcccttcg gctgaataac gacaatcaag ggcaggttga atttcttggc aaattcgaaa
     1021 tcgcgctggt cgtgagtggg aacagccata acacagccgg tgccgtaatc ggctaataca
     1081 aaattagtgg cgtaaacggg catctttttt ccggtcaagg gattttcgca atatgcctcg
     1141 aggaagatgc cttccttttc ataatactcg gaagtgcgca tcagccggtc ttgctttttt
     1201 actttctcca caaattgttg gacgtctttt tcgatggctt tgcctttgga caattccata
     1261 accagtggca gctccgcggc aatgagcatg aaggttgcgc caaaaagagt atcctggcgc
     1321 gtggtgaata tttttattgc tccttcagag ccgataagcg ggaaatccac ttcacagccg
     1381 taactttttc cgatccagtt tttctgcatg gtgagaactt tttccggcca gccggggagc
     1441 ttatcgcagt agtccagcag ctcctcgatg taggcggtga tctggaaaaa ccattgatct
     1501 aatgtttttt cattcacttc cgtgccgcag cgccagcata agccgccttc cacctgttcg
     1561 ttagccagaa cagtttcgca tttgctgcag taattgacag tggagcgttt cttgtaggcc
     1621 aggccttttt catacatcca gagaaaaaat aactgttccc agcggtaata ttgcggttcg
     1681 caagttgata tttccctgtc ccagtcgtag ctaaagccca ttcttttgag ctgcatcttc
     1741 atgtgggcga tattgtcgtt cgtccactgg gaagggtgaa ttttatgttc aatagcggcg
     1801 ttttccgcgg gcaggccgaa ggcatcccaa cccatcggat gtaaaacgtt gtaacctttc
     1861 atgcgtttgt agcgggcaac tacatcgccg atggtataat tgcgtacatg tcctatatga
     1921 attttaccgg aagggtaggg aaacatttcc agcaaataat atttttccct ttgcggatct
     1981 tccgtgactt taaaagtttt ttctttatcc cacttcgttt gccatttttc ttcaatctgc
     2041 cggggatcat acttaatatt catattgtgc tccagagtga ataatcgatg atttgtctaa
     2101 catatcgctt gagtgcttgc aatgcctttg tcattttctg ctcaattatt ttaaagatga
     2161 aaaaatattt agagcatctg gagggacatc ttatctgcgc atttcttgat cagttcatga
     2221 agcgtcgaaa gtgtcgttaa atgcgatgtg acaaagatta gattcttgac cccgttttct
     2281 acatgccgca aaaattcagt tttattcttc ttcaaaccta aaaagccata agctcccaga
     2341 gcttgcatga gccgctgtgc cgaagcctcc cggaaataaa tttcgaattc tttttcgcca
     2401 aaagttggtt ttgccaattg ataataaaaa gaaattagtt cctttctttc ttcattggtg
     2461 aaaactacat acggatcgca gataagcgaa cccaaatcat aaaagaaatt tcccaaacgc
     2521 attccctgaa aatcaatcaa cacgggctta ccgttcttga gcataatatt ctgcgattgt
     2581 aaatcacgat ggataaggca gggctctat
//
LOCUS       DASVIM010000208         4459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1447014, whole genome shotgun sequence.
ACCESSION   DASVIM010000208 DASVIM010000000
VERSION     DASVIM010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447014"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..739
                     /gene="rplM"
                     /locus_tag="VF305_04190"
                     /pseudo
     CDS             308..739
                     /gene="rplM"
                     /locus_tag="VF305_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
     gene            790..1185
                     /gene="rpsI"
                     /locus_tag="VF305_04195"
     CDS             790..1185
                     /gene="rpsI"
                     /locus_tag="VF305_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX7416363.1"
                     /translation="MSQEQRYYATGKRKSSIARVYMKAGNGNIVVNKRNYDEYFTRPS
                     LKMVIKQPFEITGKKDQFDLYVNVSGGGVAGQAGAVKHGISKALLEYDAELRPILKKA
                     GFLTRDARVKERKKYGQPGARKRFQFSKR"
     gene            1236..2273
                     /gene="argC"
                     /locus_tag="VF305_04200"
     CDS             1236..2273
                     /gene="argC"
                     /locus_tag="VF305_04200"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX7416364.1"
                     /translation="MVKVAIFGASGYTGQELARILLSHPQTELVAATSRRFAGMPVAE
                     VFPALSGLTSLKYQNATPKEIADVSDVVFLALPHGVSMDIAPVFIQAGKKVIDLSADY
                     RLRDLATYEKWYVKHSSAGILKYAVYGIPELYRQEIKKTKLVANPGCYPTSIILGLAP
                     ALKNNLLDTSTIIADSASGVSGAGREPQTDLLFCEVEGGFKAYKVGKHRHTPEIEQEL
                     NSLAGEKFAISFTPHLLPVKRGILSTIYASLKKEITLPELHSLYSSFYAAEKFVRLCP
                     AGAYPNISSVCGSNYCDIGLALDPRTKRVIIMAAIDNLIKGAAGQAVQNMNIICGFEE
                     EAGLEAVPLYP"
     gene            2273..3775
                     /gene="cysS"
                     /locus_tag="VF305_04205"
     CDS             2273..3775
                     /gene="cysS"
                     /locus_tag="VF305_04205"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX7416365.1"
                     /translation="MKVSLKNMTQKIFNTATRKKEVLQPIKEGAVGIYVCGITAYDVC
                     HVGHARAAVVFDVVVRYLRARGYQVTYVKNFTDIDDKIIDRAKKEGVSITEISERYIK
                     LHNEDMAALGVVTPTVTPKATEHMEDMIALISILVEKGYAYCVDGDVYFAINNYDQYA
                     KLSGRNLEEMIAGARVDVNDKKKNPLDFALWKKSKEGEPFWESPWGKGRPGWHIECSA
                     MSRRYLGETFDIHGGGEDLIFPHHENEIAQSQAATGKPLANYWMHNGFVKINSEKMSK
                     SLGNIFPVREILKNYQPEVLRLFMLQSHYRSPVDYSENSLAEARSGLVRCYTTLQLLK
                     EVPGNLVVDTTPITGKYSVKEENYISKFKHLADKFNAALDDDFNTAQALGYVFDAVRL
                     TNNFVADEKNMPTSNKSKILAAAKKVFDHFGTVLGIFQDDAEHFFQSDKEMESRKRGL
                     NIEKIENLIKERQTARTAKDWTKADAIRQELAGLHVVLKDTANTTSWIIE"
     gene            4132..>4459
                     /locus_tag="VF305_04210"
     CDS             4132..>4459
                     /locus_tag="VF305_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HEX7416366.1"
                     /translation="MKKLANKWPFLVIILLGLFVLVGPYHDSKVSALDKSVYKDIKTF
                     NEVFDMVKKNYVDEVDSATLIQGAINGMIRSLDPHSAFMTPEIYKELEVETQGRFGGI
                     GIEITIL"
BASE COUNT         1338 a          806 c         1049 g         1266 t
ORIGIN      
        1 gatctgttca attccagaac agtagaaaat acagtgggat tgacttatca tcagcaatgc
       61 tggagtttgg gagcagatgt tacccaaacg gatactgata ctcgggtctt gctaaaaata
      121 tcactagccg gattaggtag atggggactt tagcccactt ttttgtttac cacattttat
      181 atagtatttt agtaacacca tataaaattg gccagtaaat gcaattattt ttcaaatatt
      241 ttacttgaca agcaagctga aattacataa cttacttttt tgaatttttt accaaagtgg
      301 tgtttttatt atgaagacat atatggcgaa ggcggaagcg gcgaacaaag agtggtacat
      361 tgtggatgct tccggtaaag tattgggaag gttggctagt gaaatagcgc tccgtttgcg
      421 gggaaagcat aaagctattt atactccgca tgttgatacg ggtgatttta tcattgtggt
      481 taacgctgaa aagatttcac tcacgggcaa aaagttgacc gataagatat attattctta
      541 ttccggttat cctggcgggt tgagagaaac tgctgccggt aaaatgcttg ctgaaaaacc
      601 ggagaactta atccgcatag ctgttgccgg tatgctgccc aaaaataatc tagggtgaag
      661 gatgctcaaa aaactaaaag tttatgccgg caatgctcat cctcatgaag ctcagtgtcc
      721 gcatgcttta tccgtttaag ttacgttaag ctggagatga tctgatcgtt ttttaatatg
      781 ggagaagtta tgtcgcaaga acaacgttat tatgcaactg gtaaaagaaa aagctctatt
      841 gcccgcgttt atatgaaagc gggtaatggt aatattgtag ttaataagag aaactatgat
      901 gaatatttta ctcgtcccag tttaaaaatg gttatcaagc agccctttga aattacaggg
      961 aaaaaagatc agtttgattt atatgttaat gtaagcggcg gtggggtagc cggtcaagct
     1021 ggggcggtaa aacatgggat ttctaaagct ctcttggaat atgatgccga gcttcgccca
     1081 attctgaaga aagcgggctt tttaaccaga gatgcccgcg tcaaggaaag aaagaaatac
     1141 gggcagccgg gagcaaggaa aagatttcaa ttctccaagc gttaatttca acaagggggg
     1201 cttcaggcct cccttttttt tggaggatta ttattatggt aaaagtagca atatttggag
     1261 ctagtggata taccggtcag gaattggcaa ggattctgct ttcgcatccg caaacggaat
     1321 tagttgccgc aacatcacgg cggtttgccg gaatgccggt agcggaagta tttccggcgc
     1381 tatccggttt aacatcacta aaatatcaaa atgcaacgcc taaagagata gctgatgtaa
     1441 gcgatgttgt ttttctggcg ctccctcatg gcgtttccat ggatattgct cctgtcttta
     1501 ttcaggccgg gaaaaaagta attgatttaa gtgccgatta tcgcttacgg gatttggcga
     1561 catacgagaa gtggtatgtc aagcacagca gcgccggtat tttaaagtat gcagtttacg
     1621 gaattcccga actttaccgg caggaaataa agaaaaccaa acttgttgcc aatccgggct
     1681 gctatccgac cagtattatc cttggtctgg cgcccgcgtt gaaaaataat cttctggata
     1741 cgtccacaat tattgctgat tctgcttccg gcgtgagtgg ggcgggacgg gaaccacaaa
     1801 ccgatttgct tttttgtgaa gtggaaggtg gttttaaagc atacaaagtc ggcaaacaca
     1861 ggcatactcc cgaaattgaa caggaattga actctctggc tggtgaaaaa ttcgctattt
     1921 cttttacgcc gcatttattg cccgtaaaac ggggcatttt gagcacaatt tatgcgtcac
     1981 tgaagaaaga gattacttta ccggagctgc attcccttta ttcctcattt tatgctgcag
     2041 aaaaatttgt gcggctttgt cccgccggag cgtatccgaa tatttcttca gtttgtggat
     2101 caaattattg tgacataggt ctggctcttg atccgcggac aaaacgggtc attattatgg
     2161 cagcaattga taatttaatt aaaggcgccg cggggcaggc cgtgcaaaat atgaatatca
     2221 tctgcggttt tgaagaagaa gctggtttag aagccgtacc gctctatccc tgatgaaagt
     2281 aagtttaaaa aacatgactc agaaaatatt taatacggcg acaagaaaaa aagaagtctt
     2341 acagcctata aaagaaggag ctgtcggtat ttatgtttgc gggattacag cttacgatgt
     2401 ttgccatgtc ggtcatgccc gcgccgcggt ggtgtttgac gtggtggtcc ggtatttacg
     2461 agcgcgcggc tatcaggtga cctatgtcaa aaactttacc gatattgacg ataaaattat
     2521 cgatcgcgcc aaaaaagaag gcgtcagtat tactgaaata tcggaacgtt acatcaagtt
     2581 gcacaatgaa gatatggcgg cgctgggcgt tgtaactcct acggtaaccc cgaaggccac
     2641 cgagcacatg gaagatatga ttgctctgat cagcattctc gtggagaaag gatacgctta
     2701 ttgcgtggat ggggacgtat attttgcgat aaataattac gaccaatatg ctaagctttc
     2761 cggacgtaat ttggaagaaa tgatcgccgg cgcgcgcgtt gatgtcaatg acaaaaaaaa
     2821 gaatcctttg gattttgcgc tctggaaaaa aagtaaggaa ggcgaaccat tctgggaaag
     2881 cccgtggggc aaaggacgcc ctggctggca tatagaatgc tctgctatga gtagacgtta
     2941 tttgggggaa acattcgata ttcatggcgg tggcgaagat ttaattttcc cacatcatga
     3001 aaatgaaatt gctcaatcgc aggccgcgac agggaaacca ctggctaact actggatgca
     3061 taatggtttt gttaaaatca attcggaaaa aatgtctaag tctttgggta atatctttcc
     3121 tgtcagagaa atcttaaaaa attatcagcc ggaagtattg cgcttattta tgctgcagag
     3181 tcattaccgc agtccggttg attattcgga aaattcttta gccgaggcaa gatcaggact
     3241 ggtcagatgt tatacgactt tacaactcct gaaagaagta ccgggcaatt tggttgttga
     3301 cacaacaccg attacaggga aatattcagt aaaagaagaa aattacataa gtaaatttaa
     3361 acatttagcg gataaattta atgcggcgct ggatgacgat tttaacaccg ctcaagcttt
     3421 gggttatgtt tttgatgcgg tacggctcac aaataatttt gttgctgatg aaaaaaatat
     3481 gcctacttct aataaatcta aaattttagc ggcggcaaag aaagtatttg accattttgg
     3541 tacagtttta gggatttttc aggatgatgc ggaacatttt ttccaatccg acaaggaaat
     3601 ggagtcacgt aaaagaggat taaatattga gaaaattgag aatttgatca aggaaaggca
     3661 aactgcccgc acggcgaaag attggactaa agcagatgcc atcagacagg agttggcagg
     3721 gcttcatgtt gtcctgaaag acaccgcaaa tacaaccagt tggataatcg aataattaat
     3781 accacttggc ttgtaagtta ccactttgca agtggtaatt accggtaagg taaaaactta
     3841 tttattatac gctcccctgc gggacgctta accggtcttt ggctaatttt gtttggctgc
     3901 gtcgtcggct tcctcaacgt ataacctgaa ggtcactcgt gtaaaatacg cctcgtcgcc
     3961 tccttcttgc cgcttcgtta tccttaatta aagcgaaatg gtggtataag catcagtata
     4021 atttgatctg atttaagctc ttcatgtttt ctcaatattt ccgaaatata tggaaaatat
     4081 caataaaaat gatataataa taaaattatc ttctgaattg agaggaattt tatgaaaaaa
     4141 ttagcaaata agtggccgtt tttggttatt attctcctcg ggttattcgt cctggtggga
     4201 ccttatcatg atagcaaggt ttccgcgctg gataaaagtg tctataaaga cattaagacg
     4261 ttcaatgaag ttttcgacat ggtaaagaaa aactatgttg acgaggtgga ttcagcaacg
     4321 ttaattcaag gtgcaatcaa cggaatgatc aggtcattgg accctcacag cgcgtttatg
     4381 acgccggaga tatataaaga gctggaagtg gaaacacagg ggcgtttcgg gggcattggt
     4441 attgaaatta caatattaa
//
LOCUS       DASVIM010000209         6289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1532709, whole genome shotgun sequence.
ACCESSION   DASVIM010000209 DASVIM010000000
VERSION     DASVIM010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532709"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VF305_04215"
     CDS             complement(<1..399)
                     /locus_tag="VF305_04215"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HEX7416367.1"
                     /translation="MSYTRNKSWYLLIGLLLAVLSGIMLFGFTQAASAEGRGFGPHEF
                     MDSRYHHEHAYPVRGHFVDALPRGHHVAVYGRSRYHFYGGVWYRPYGRRFAVVAPPFG
                     LIVPFLPLYYTTLWVGGIPYYYANEVYYTQT"
     gene            complement(442..729)
                     /locus_tag="VF305_04220"
     CDS             complement(442..729)
                     /locus_tag="VF305_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416368.1"
                     /translation="MPPPGHDTAILAIAGAVLGALIAGPRHAGAGALIGAGSGAVAGA
                     VSDATRQQYAQQMEEAYANRDQALDTRYEGKAREFRRAMSACLEGRGYSVQ"
     gene            complement(1367..2377)
                     /gene="amrS"
                     /locus_tag="VF305_04225"
     CDS             complement(1367..2377)
                     /gene="amrS"
                     /locus_tag="VF305_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX7416369.1"
                     /translation="MIKEAMLYEKLDDKRVHCNLCAHRCTIKPDRRGICGVRENKDGV
                     LYTLVYGTLIAENIDPIEKKPFFHVYPGSLSYSIATVGCNFSCEFCQNHEISQMPRST
                     LMITGKDILPAEIVAQAKKSGAKTIAYTYTEPTIYFELAYDTAKIAHENGLKNVFVTN
                     GFMTPVAIDTIAPYLAAANVDLKSFSDDFYKKQCGARLKPVLESLKKMKELGIWVEIT
                     TLLIPSLNDSDKELKDIAQFIAGLGKETPWHISRFHPQFKMHSLPVTPVASLHRAVET
                     GKQSGLEYVYSGNVPGDEGENTNCFHCGNRLIERHGYKIISINLTGNKCSNCGTELEG
                     IF"
     gene            complement(2374..3156)
                     /locus_tag="VF305_04230"
     CDS             complement(2374..3156)
                     /locus_tag="VF305_04230"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7416370.1"
                     /translation="MVFDQRAANLYDERLKTSLGQYIFSRQKELILELVAPRAGERML
                     DVGCGTGNHLQVFREKWCSVTGIDSSTAMLEIARKKMGTHIDLQLGKAEDLPFSDDEF
                     DIVTMINALEVAQNPKKAIAEAIRVCRGRIFIGFLNNCSFVGTKQMLKKIFGFPISEK
                     IRFFGIEEIKEMVGSIIAPSEIKWGSVIYFPTIVYDIATELEELVPHAKNPLGAFVGL
                     TFPVKYTYRTAQSPIMESFKLNAEARRTAPEAVRGMLQKADK"
     gene            complement(3304..4368)
                     /gene="moaA"
                     /locus_tag="VF305_04235"
     CDS             complement(3304..4368)
                     /gene="moaA"
                     /locus_tag="VF305_04235"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX7416371.1"
                     /translation="MSSCIVVTIMLFVFKILKVSAAETMHDKFDREINYLRVSITDRC
                     NLHCRYCRPKEGISLQGHDDILRYEEIIRIVSIAVKLGLIKVRITGGEPLVRRGFIEF
                     ITALKNINGLQDISLTTNGILLEEFAQNIFDSGIRRINVSVDSLNKDKYFRITNGGNL
                     EAVLRGIAKAEKVGFSPIKINTVAIKGFNDDEVLAFAQLAADKPFQVRFIELMPVGQA
                     NSDYREDYLPAMQLMKEISGRYELEKIKDKKNRSDGPAKIFKIKGGRGEIGFINPVSS
                     HFCSTCNRLRLTSDGKLRACLLNEEEVDLKKALRENWSDTELERLIREAILLKPRQHD
                     LACTERHLKKCHRDMSVIGG"
     gene            4481..4960
                     /gene="greA"
                     /locus_tag="VF305_04240"
     CDS             4481..4960
                     /gene="greA"
                     /locus_tag="VF305_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX7416372.1"
                     /translation="MEKIPITKAGFEKLKKDLEKLKSVSIPENIRDIEVARAHGDLSE
                     NAEYSAAKERQSFLYGKVQELENNLATSNVINLKGLTNEKVVFGCYVSMEDTDSGEKI
                     KYRLVGPYESDINQNKISVTSPIGKAVIGKCVGDEINVKTPGGQRNLEVVKISLDEN"
     gene            complement(5048..5452)
                     /locus_tag="VF305_04245"
     CDS             complement(5048..5452)
                     /locus_tag="VF305_04245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX7416373.1"
                     /translation="MADELMLEIVTPEKMAFSGKVEEVTIPGTEGEFGVLRGHEAFLS
                     SVDTGELNFTKDSKKTHYAVNTGYAEVTANKVTILIETAERSDHIDKDRARRARENAE
                     ARLEKIAKDNVEYDKARAALMRAIVRLNVAEK"
     gene            complement(5473..>6289)
                     /gene="atpD"
                     /locus_tag="VF305_04250"
     CDS             complement(5473..>6289)
                     /gene="atpD"
                     /locus_tag="VF305_04250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7416374.1"
                     /translation="EMKQSGVLKQAALIYGQMTEPPGARARVALTGLTAAEYFRDEEG
                     QDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATDLGELQERITSTTKGSITA
                     VQCVYVPADDLTDPAPATTFAHLDGTVVLSRPIAELGIYPAVDPLDSTSRILDPLVLG
                     QEHYQVARQVQVTLQKYKDLQDIIAILGMDELSEADKLTVSRARKIQRFLSQPFFVAA
                     QFTGTEGKFVSVADTVRGFKEILDGKHDDLPEQAFWLVGGIEEAVEKAKKISQ"
BASE COUNT         1682 a         1501 c         1313 g         1793 t
ORIGIN      
        1 ggtttgagta taatatactt cattggcata atagtaaggt ataccgccta cccagagagt
       61 cgtgtagtac agtggcagaa agggaactat aagaccgaac gggggtgcga cgacagcgaa
      121 gcgccggccg tacgggcggt accaaacacc tccgtagaag tggtatcgtg agcggccata
      181 aaccgccaca tggtgacccc tcggcagcgc atcaacgaaa tgaccgcgaa ccggataagc
      241 atgttcatgg tgataacgtg aatccatgaa ttcatgaggg ccgaatccgc gcccttccgc
      301 acttgctgcc tgcgtaaacc cgaaaagcat gatccccgac aacactgcca gcagaagccc
      361 gatcaaaaga taccaactct tattacgagt ataggacatg gttattacct ccataaaatc
      421 aaatgtagct attagtcttc cttattgcac actatagccg cggccttcca ggcaagccga
      481 catcgcccga cggaattcgc gcgcttttcc ctcatagcgg gtgtccagcg cctgatcgcg
      541 attggcataa gcttcttcca tctgctgagc atactgttgc ctggtcgcat ctgatacggc
      601 gccggcaaca gccccgcttc ctgcaccgat caatgcgccg gccccggcgt gcctcggtcc
      661 ggctattaac gcgcccagta ccgcaccggc gattgccaga atggcggtat cgtgacctgg
      721 aggaggcatt ggcaccactt tgacgcgacg ttcgggcgga atcgatgact gactcggatc
      781 gaagccggtc tgttgcatcg cccaattgta acattcatag tggtctcgcg actgctgctc
      841 tttcgtctgg ccctctttcg ggtaaaaata gatctgggta agaggagtcc ttggggctgg
      901 gcttgcgctt ccgacctcgc ggtaagtcgc agggtaacac gcagtaagtg ttaggattaa
      961 taagaaaagc aaaagccgaa ggcttgctct tgataaccgc cgtttctgta tatgttttcc
     1021 tgtcgtcatc ttccagattg acatcatcat tatttatgcc ctccttcgta gttaaacacc
     1081 agagaaatga tcatcacaaa aggatgattt tcccccggtg tttattattc tttctctttc
     1141 atgagtttta aatctatctt agataataat taaaagcaag gggtgtgcca gtcctatcat
     1201 tttaaagtgc aacatattga attaatattg atattatagt tttatagaaa tcagtaagct
     1261 tcctttgaag acataaatat gggtaataag tatccaactt tagtagccac ttgtagacaa
     1321 aatatccacc cgcgatagtg gcagtatttg ataagattat ttgttcttag aaaatgcctt
     1381 ccaattccgt accgcagttg gagcatttat ttccggtcag attgatgctg atgattttat
     1441 aaccatggcg ttcgattaaa cgattgccgc aatggaaaca atttgtgttt tctccttcat
     1501 ctccgggtac attgccgctg taaacgtatt ccaatccaga ctgttttcca gtttccactg
     1561 cccgatgcag tgaggccaca ggggttaccg gcaagctgtg cattttgaac tgcggatgaa
     1621 agcggctgat atgccagggg gtttcttttc ccaatccggc aatgaattgc gcgatgtctt
     1681 ttaattcttt atcgctatcg ttcagagagg gaataagtaa ggtagttatt tccacccaaa
     1741 ttcccagctc cttcattttt ttcagactct ccaaaactgg tttgagcctt gccccgcatt
     1801 gtttcttata aaaatcatcg ctaaacgatt tcaaatccac attggcggcg gccaaatatg
     1861 gggcaatagt atcaatcgct acgggagtca taaagccgtt ggtgacaaac acatttttta
     1921 aaccattttc atgggcaatt ttagcggtat catatgccaa ttcaaaataa attgtcggtt
     1981 cagtgtaagt ataggcgatt gttttcgcgc cgcttttttt cgcttgggct acaatttctg
     2041 caggcagaat atctttgccg gttatcatga gcgtagagcg cggcatctgc gaaatttcat
     2101 ggttctgaca aaattcacaa ctgaaattgc atccgacggt ggcaatagaa taggaaaggg
     2161 agcccggata aacatggaaa aaaggttttt tttcgatagg gtcgatattt tcggcaatca
     2221 gcgttccgta aaccagtgta taaagaacgc cgtccttatt ttcccttact ccacagatgc
     2281 cccgcctgtc cggtttaatt gtgcatctat gtgcgcataa attacagtgc acgcgcttat
     2341 catcgagctt ttcataaagc atcgcctctt tgatcattta tcggccttct gcaacatgcc
     2401 cctaacagct tcaggagctg ttcttcgggc ttcggcattt agcttaaatg attccataat
     2461 cgggctttgc gctgtacgat aagtgtattt cacagggaat gtaagcccga caaacgcacc
     2521 gagcggattt ttcgcatgag gaaccagctc ttctagctcg gtagcaatat cataaactat
     2581 ggtcggaaaa tagataacac tgccccactt tatttctgat ggggcaatta tgcttcccac
     2641 catttctttt atttcctcaa taccgaaaaa acgtattttt tcggagatgg gaaatccgaa
     2701 aatttttttc aacatttgtt tagttccgac aaaagaacaa ttgtttaaaa atccgataaa
     2761 aatccggccg cggcaaacac ggattgcctc ggcgatggcc tttttagggt tctgtgcaac
     2821 ctcgagggca ttaatcatag taacaatatc aaactcatcg tcggaaaaag gcaggtcttc
     2881 ggccttaccc agctgcaagt caatatgggt gcccattttt ttccgggcaa tttccagcat
     2941 tgcggtggag gaatcaatcc ccgtcaccga acaccatttt tcgcggaaaa cctgcaggtg
     3001 attgccggtg ccacacccca cgtccagcat cctctcgccc gcgcggggag caaccaactc
     3061 caaaataagc tctttttgcc gggaaaagat gtattgcccg agggatgttt ttaatctttc
     3121 atcatacaaa ttggctgctc tttgatcgaa aaccatttaa cctatccttg aatcctaaac
     3181 atttacctca aattcgatat cgatttccct ggcggttcta ccaaccagca tctccatttt
     3241 taccaccgtt ataataaact atactccgtt ttttataaat gtatcttctt tatttctttt
     3301 ttttcaacca ccaattaccg acatgtcacg gtggcatttt ttcaaatgtc tttccgtaca
     3361 ggccagatca tgttgcctgg gttttaacaa tatcgcttcc cggataagcc tttccagttc
     3421 tgtatcgctc caattttccc gcaaagcctt ttttaagtca acttcttctt cattaagtag
     3481 acatgctctt aatttcccgt cagaagtaag acgcaaacga ttgcaggtcg aacaaaaatg
     3541 gctgctcacc ggattaataa aaccaatttc gccgcgccct ccttttattt taaaaatttt
     3601 tgccgggccg tctgatctgt ttttcttatc ttttatcttc tctaactcgt agcgaccgct
     3661 gatttctttc attaattgca ttgccggcag ataatcttcg cgataatctg agtttgcctg
     3721 ccccacgggc ataagttcaa tgaaacgcac ttgaaaaggc ttatccgcag ccaattgggc
     3781 aaaagcaagc acttcatcgt cattgaaacc ttttatcgct actgtgttaa tttttatcgg
     3841 cgagaaacca accttttcag ccttggcgat tccgcgcaaa acggcctcta aattcccgcc
     3901 attcgtgatg cggaagtact tatctttgtt cagtgaatcg acgctgacgt taatacggcg
     3961 gataccggaa tcaaaaatat tttgggcaaa ttcttccaac aggataccgt tggttgtgag
     4021 gctgatgtct tgtaagccgt tgatgttttt taaagctgta atgaactcaa taaagccgcg
     4081 gcgcacgagc ggttcgccac cggttattcg aaccttgatc aatcctagct ttacggcaat
     4141 ggaaacaata cgaattattt cttcataccg gagaatatca tcgtggcctt gcaaagaaat
     4201 tccctctttg ggccggcaat acctgcaatg caaattgcac ctgtcggtaa tggaaacgcg
     4261 caggtaattt atttcccggt caaatttatc atgcatagtt tcagcagcgg agacttttaa
     4321 aattttgaaa acaaataaca taatcgtaac aacaatacaa cttgacatca aatgtttctt
     4381 gacagggtgc ctattctcta ttaaacaaaa attagcaatt cccctgcctg ttgttttgaa
     4441 taaatcatgt cagagcaagg caaaataatc tggagttatt atggaaaaaa taccgattac
     4501 taaagcaggt tttgaaaaat taaaaaagga tttggaaaaa ttaaaaagcg tatccattcc
     4561 tgaaaatatt cgcgatattg aagttgcccg cgcgcacggt gatctttccg aaaacgccga
     4621 atactccgca gccaaggaac ggcaatcttt tctgtacgga aaggtgcagg aactggaaaa
     4681 caatttagct acttctaacg ttattaattt aaaaggctta actaacgaaa aggttgtttt
     4741 cggctgctat gtcagcatgg aagatacgga cagcggtgaa aagatcaaat accgactcgt
     4801 tggcccttat gaatcggata ttaatcaaaa taagatttcc gtaacctcgc ctatcggaaa
     4861 agcggtaatt gggaaatgcg ttggcgatga aatcaacgtt aagacacctg gcggccaacg
     4921 gaatctggaa gtagtcaaaa tttcgcttga tgagaattag ctagtaattg ctggttagta
     4981 tcaatactta aataaaaggc cgcacattta ataaatgcac ggccttttat ttttgacttt
     5041 aataatttta tttttcagcg acgtttaaac gtacgattgc ccgcatcaat gcagcgcgtg
     5101 ctttatcgta ttcgacatta tctttggcta tcttctccaa gcgggcttcc gcattttccc
     5161 ttgctcttct ggctctatct ttatcaatat gatcggacct ttcggctgtt tcgattaaaa
     5221 ttgtaacttt attggcagtg acttccgcat aaccggtatt gaccgcataa tgggtctttt
     5281 tgctgtcttt agtgaaattc aactcgccag tgtctacgga actcaaaaat gcttcgtggc
     5341 cgcgcaacac accgaattca ccttccgtac ccggaatggt aacttcttcc accttgcccg
     5401 aaaaggccat tttttccggc gttactattt ccagcatcaa ttcatccgcc atttacttcc
     5461 tccaacttaa aattattggg aaatcttctt ggccttttcc acagcttctt caattccacc
     5521 gaccagccag aaagcttgtt cgggcaaatc atcgtgtttg ccatccaaaa tttccttgaa
     5581 acctctgacc gtatcggcaa ccgacacaaa ttttccttcc gtcccggtaa attgcgcagc
     5641 aacaaaaaaa ggctgcgaca agaatctttg aatctttcta gccctgctta cagttagctt
     5701 gtcggcttcg gagagttcat ccatacccag aatggcgata atatcctgta aatccttata
     5761 tttttggaga gttacctgta cttgccgcgc tacctgataa tgttcttgtc ctaaaacgag
     5821 aggatccaaa atacgcgacg tcgaatccaa cggatcgaca gccggataaa taccaagttc
     5881 tgcaatcgga cgcgacaaaa cgaccgttcc atcaagatgc gcaaaagttg tagcgggcgc
     5941 cggatcggtc aagtcgtccg caggcacgta aacgcactgg acagccgtaa tagaaccttt
     6001 ggttgtcgaa gtgattcttt cctgcagttc gcccaggtct gtagccaacg tcggctggta
     6061 cccgactgcc gaaggcatgc gtcctaaaag agcggaaact tccgaaccgg cctgcgtaaa
     6121 gcggaaaata ttatcaatga ataaaagcac gtcctggcct tcctcgtcgc ggaaatattc
     6181 cgctgccgtc aatccagtga gggccactct ggctcgggct ccgggcggtt cggtcatctg
     6241 accgtaaatc aaagctgctt gtttaagtac gcccgactgt ttcatttcc
//
LOCUS       DASVIM010000210         3363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_424555, whole genome shotgun sequence.
ACCESSION   DASVIM010000210 DASVIM010000000
VERSION     DASVIM010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424555"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VF305_04255"
     CDS             complement(<1..617)
                     /locus_tag="VF305_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7416375.1"
                     /translation="MLSKKWRITVLALFTVALFPHPVSAQEPLTLEASVEIALKNSIV
                     INIAKEGSKGATAQKREAITGCLPKFSTSYNYTRLNEAPFSRFQGLPPGPLSGLNGME
                     IPVGTVNNYNWVIEAKQPLFAGGGILANYQASIIGEDTALLEEKAQYQDVVQEVKIAY
                     FNILRAQRIQDAARQSVEMLSAHRDVAENYFKVGMIPKNDLLHAEV"
     gene            complement(607..1227)
                     /locus_tag="VF305_04260"
     CDS             complement(607..1227)
                     /locus_tag="VF305_04260"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7416376.1"
                     /translation="MERLERKEREFNTRRADILEQAERIFAAKGFYNVTMAEIAGASG
                     FSIGSLYQFFEGKENLYTTMVGEKLDLMYAGIREEVNATKDITDKIAALIGAHFRFVE
                     KNTDFCRIFLRGENDALSEMMTSLRRKLIDDYLQHITFIENLMKSGIKKGFLRPLVPR
                     EMAGLLFGVIRSTAIDWMLLPTKESLNLKKDRIMEIFLEGVKKHAQ"
     gene            1571..3070
                     /gene="glpK"
                     /locus_tag="VF305_04265"
     CDS             1571..3070
                     /gene="glpK"
                     /locus_tag="VF305_04265"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX7416377.1"
                     /translation="MKKLYILALDQGTTSSRAILYDRDGKVVKITQKEFTQIYPKAGW
                     VEHDPMEIWGSQSGVVSEILATTGIASEEIAAIGITNQRETTVVWEKQTGKPVYNAIV
                     WQCRRTSAICDELKARGLENYIRENTGLVLDAYFSGTKVKWILDNVEGARQKAKNGEL
                     LFGNIDTWLIWNLTRGKVYVTDYSNASRTMLYNIKDLKWDEQILKELDIPASMLPEVK
                     PSSAVYGNTDARIFGAEIPIAGDAGDQQAALFGQACYSPGMAKNTYGTGCFMLMLTGE
                     KLVHSKNGLLSTIAWGVNNKVEYALEGSIFIAGAAVQWLRDSLKVIYDAKDSEYFATK
                     VDDSLGVYVVPAFVGLGAPYWDMYARGAILGLTRGTTRNHIIRATLESIAYQTRDVLE
                     LMHSECGIELRELRVDGGACANNFLMQFQADILGVPVERPEIIETTALGAAYLAGLAV
                     GFWKDQSMIAERRKVNRKFNPIMSEDKRERLYTNWKKAVKRAMHWEEEE"
BASE COUNT          974 a          740 c          781 g          868 t
ORIGIN      
        1 acttcggcat gcagtaaatc atttttggga atcataccga ccttgaaata attttcggca
       61 acatcccgat gcgcgctaag catctcgacc gactgacgcg ccgcatcctg aatcctctgc
      121 gcccgcaaaa tgttgaaata agcaatcttt acttcctgca caacgtcttg atactgggct
      181 ttttcttcca agagagcggt gtcttcacca attatacttg cctgataatt ggccaaaatt
      241 ccgcctccgg caaaaagcgg ctgctttgct tcaatgaccc agttatagtt atttactgtg
      301 cctacgggaa tttccattcc attcaagcca gaaagaggcc ccggaggaag gccctgaaat
      361 ctggaaaacg gcgcctcatt caatcgggta taattataag atgtgctgaa cttgggcaaa
      421 catccggtaa tcgcttccct tttctgggca gtagcgccct tggacccttc tttggcaata
      481 tttataacaa tactattttt caaggctatt tcaacgctcg cctctaaggt taaaggctct
      541 tgtgcggaaa ctggatgcgg gaaaagagca actgtaaaaa gtgctagaac agttatgcgc
      601 cattttttac tgagcatgct tttttacccc ctctaaaaat atttccataa tacgatcttt
      661 tttgaggttt aatgattctt tcgtaggcaa taacatccag tcaatcgctg tagacctaat
      721 taccccaaat aacaatccag ccatttcacg aggcacaagt ggccgtaaaa agcccttctt
      781 tatgccgctt ttcattaggt tctcgataaa agttatatgc tgcaaataat catcaattaa
      841 tttccgtcgc aatgaagtca tcatttccga taaagcatca ttctccccgc gcaggaatat
      901 gcggcaaaaa tctgtgtttt tttcgacaaa ccggaaatgc gcgccaatca gcgcggcaat
      961 tttatcggtg atatcttttg tcgcgtttac ctcttctctg attccggcat acatcaaatc
     1021 gagcttttcg ccaaccatag ttgtgtaaag attctctttg ccttcaaaga attgataaag
     1081 cgacccaatg gaaaaacccg acgcaccggc tatttccgcc attgttacat tataaaatcc
     1141 tttagcagcg aatattcttt ccgcctgctc cagaatatcc gctcgacgcg tattaaactc
     1201 gcgctctttt cgttccaacc tttccattaa ccaggccttt ctatttttga acaatcattc
     1261 aatatttgaa tgacgattca gattatagaa tatttattat ttgtcaagtg gaaattttta
     1321 gtttttctcc aaaggcgaaa aaatttaaat gtcatttgtt actttgcttt acttgaggat
     1381 gaattccaaa cgtgctatag ggcaattaag ggagtaaaag ggcaaattta taaatttgat
     1441 atttgggaat tactgcacac actaaaaaaa gtaattccag aagcaatttt catttggcaa
     1501 atacaaaacg ttatgtaata ctaatcttaa taaaagcatt tatatttact ctaacattag
     1561 gagtgcgaat atgaaaaaat tatatatatt ggcccttgat cagggcacta ccagttcgcg
     1621 cgccattctg tacgaccgcg atggaaaagt cgtaaaaatt acccaaaaag aatttactca
     1681 aatctaccct aaagccggct gggtggaaca tgatcctatg gaaatctggg gatctcagag
     1741 cggcgtggtg agcgaaatcc tggcaacgac tggtattgcg tccgaagaga tagcggccat
     1801 cggcatcacc aaccagcggg aaacaacggt tgtatgggaa aaacaaacgg ggaagccagt
     1861 ttataatgca attgtgtggc agtgccggcg cacctctgcc atatgcgatg aactcaaagc
     1921 ccggggcctg gaaaattata tccgggagaa caccgggtta gtgctggacg cctatttttc
     1981 cggcaccaaa gtcaagtgga tactggataa cgtggaagga gccagacaga aggcaaaaaa
     2041 cggcgaactg ctttttggca atattgacac ctggttgatc tggaacctga cgcgggggaa
     2101 agtttacgtt acggattata gcaatgcttc acgcacgatg ctttacaata tcaaggacct
     2161 taagtgggat gagcaaatct taaaagaact cgacataccg gcttccatgc tgccggaagt
     2221 aaagccatcc agcgcggttt acggcaatac ggatgcccgg atatttggag cggagatacc
     2281 cattgccgga gatgcaggag atcagcaggc ggcgctgttt ggacaggcct gctactctcc
     2341 cggaatggct aagaacacct acggcacggg ctgtttcatg ctgatgctaa ccggcgaaaa
     2401 gctggttcat tcgaaaaacg gacttctttc gaccattgcc tggggagtga acaacaaagt
     2461 tgaatatgcc ctggagggca gcatttttat tgccggtgcc gcggtgcaat ggctgcgtga
     2521 cagcctgaaa gtaatttatg atgcgaaaga cagcgaatat tttgccacaa aagttgacga
     2581 ctccttaggt gtttacgttg ttcctgcctt tgtgggactg ggtgctcctt actgggacat
     2641 gtatgcccgg ggcgctattt taggtttgac cagagggaca acgagaaatc acatcatccg
     2701 cgccactttg gagtccatcg cctatcaaac ccgggatgtt ctggaactga tgcacagcga
     2761 atgcggcatt gagctgcgcg agctgcgcgt ggacggcggg gcctgcgcca ataatttcct
     2821 gatgcaattc caagcggaca ttcttggtgt tcccgtggag cggccggaga tcattgaaac
     2881 aacggcttta ggggcggcct atctggccgg cctggcggtc ggtttctgga aagatcaatc
     2941 gatgatcgcc gaacggcgga aggtaaatcg aaaattcaac cctatcatga gtgaagataa
     3001 aagagagagg ctctatacca actggaaaaa agcagttaaa cgagccatgc attgggagga
     3061 agaagaataa caaaacatgc ttaaaactga cgttacaaag gttcaccagg aaaaagattg
     3121 acacagaaag aagaaagcat ggaaaaatac aggggaaaga tttttatgaa gcagtcccat
     3181 caacttttca taaatcggag gaaattgcgg aaatgaagaa gaagatcgcg gtaataggtt
     3241 cgggtaattt cggcacggcg gttgcgcggc acgtggcacg gaatgtcgcc caaaaaaaag
     3301 gtttgcaaaa tctatggcgc aagcaggtcg atatgtgggt tatggaggaa acgctagatg
     3361 acg
//
LOCUS       DASVIM010000211         3271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_508729, whole genome shotgun sequence.
ACCESSION   DASVIM010000211 DASVIM010000000
VERSION     DASVIM010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508729"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VF305_04270"
     CDS             complement(<1..400)
                     /locus_tag="VF305_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416378.1"
                     /translation="MKRNIYVFAGIFVMSLFLSSCMVAEDKYMKKSEEADNLSKEVAA
                     QKEKNKALSDENTALKESTSKLEKKSKEVEATSNTYKELLQEMKGEIAQGQVTITELK
                     GKLTLDVVDKILFDSGESAVKEEGLAVLKRV"
     gene            complement(596..1531)
                     /locus_tag="VF305_04275"
     CDS             complement(596..1531)
                     /locus_tag="VF305_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX7416379.1"
                     /translation="MRKWQFFVFSLFLSLCFINSSLAREYTVKTGDSLKSISKESGIS
                     VNEIKEANNLTKTILRPNQVLIIPEKSVAVSTAGPKPKVKRAVYYTVKNGDTLSGISR
                     KTGVSVKQIMALNNIRPKSLRLGRKLVLANSTLLLENGNTESDKDLLHNEDEEDAAKT
                     GELADLEKENQAHEELLGKWHNADERQLFVKVATGFLGAPYRLGGSSLKGIDCSSFVR
                     KIYQIFDIDLPRNARAQSKVGMSIDREELVEGDLVFFRTRRPLGHVGIYIGNNEFVHA
                     SSRRKVVRIDSMDTPYFQKRFQRAVRIKGLDNKGG"
     gene            1806..2555
                     /gene="mtgA"
                     /locus_tag="VF305_04280"
     CDS             1806..2555
                     /gene="mtgA"
                     /locus_tag="VF305_04280"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX7416380.1"
                     /translation="MKLWKKIFFIALFLTVTFFIVDIGRYFFYPNVAYLKKHNPGKTA
                     FMEYREKTWQQKKLKKEIRKIWVPISEVSPYVMKAVIISEDSKFWSHEGFDFEAMQKA
                     LEKDIKKNKIQAGGSTISQQLAKNLYLSPDKDFIRKLKEAILTWRIENQLSKRRILEL
                     YLNVVEWGDGVFGIEAAAQKHYGKSAAQLNAREAARLASILPNPRRYKPDDMSRYVED
                     QSERIYQIMVRQGIVIPEYDEVITEKNGNSH"
     gene            complement(2885..3034)
                     /locus_tag="VF305_04285"
     CDS             complement(2885..3034)
                     /locus_tag="VF305_04285"
                     /inference="COORDINATES: protein motif:HMM:NF022739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEX7416381.1"
                     /translation="MTLNLSIAPLLALIAGIIILIVPKMLNYVVAIYLIAIGILGILG
                     YGHYR"
BASE COUNT          978 a          636 c          652 g         1005 t
ORIGIN      
        1 ccactctttt gagcaccgcg agaccttctt ctttcaccgc tgattcaccg gaatcaaaaa
       61 ggattttatc cacgacatct aaagtgagct tgcctttaag ttcggtgatg gtaacttgtc
      121 cctgagcaat ttcgcccttc atttcctgga gcaattcttt ataagtattg cttgttgctt
      181 cgacttcctt tgattttttt tctaatttgg aggtgctttc cttaagagcg gtattttcat
      241 cagagagcgc tttatttttt tctttttggg cggctacttc cttggacaaa ttatcagctt
      301 cttctgattt cttcatatat ttgtcttccg caaccatgca actgctgaga aatagggaca
      361 taacaaaaat accagcaaaa acgtaaatat ttcttttcat gtaattgctc cttatttttt
      421 agtacaaatt attattctat cactaaaaag ccgggaacag accaggctaa tagatatgaa
      481 ttcatataat aaacttgctg gataatcaac tggcaaaaac cttccgggag aaaaattaaa
      541 aaagggcgga ttgttccagc cctttttaat aaaataaaat tatggtggcc cgcctttacc
      601 cccccttgtt atccagccct tttattctga cggcgcgctg aaatcttttt tgaaaatagg
      661 gagtatccat gctgtcaatg cgaacaactt ttcttctaga ggacgcgtgg acaaattcat
      721 tattgccgat gtaaattccc acatgtccca gaggcctgcg tgtacggaaa aaaacgagat
      781 cgccttccac cagctcctcg cggtcgatgc tcatgccgac tttggattgc gcgcgggcgt
      841 tacgcggtaa atctatatcg aaaatttgat aaatcttgcg gacgaaagag gaacagtcaa
      901 tacctttgag gctcgagccg cctagtctgt atggcgcgcc caggaagccg gttgccactt
      961 taacaaataa ttgtctttca tcggcgttgt gccattttcc taataattct tcgtgcgctt
     1021 gattttcttt ttccaaatcg gctaactcac cggttttcgc ggcgtcttct tcgtcttcgt
     1081 tgtgcagtaa atctttgtcg ctttctgtat ttccattttc taagagcaga gttgagttgg
     1141 ccagaactaa tttccggcca aggcgtaaac ttttcgggcg gatattgtta agagccatta
     1201 tttgttttac ggaaactcct gtttttcggg agattccaga cagtgtatct ccatttttga
     1261 cagtgtaata aacagcccgt ttaaccttcg gtttcggccc ggcagtagaa actgcaacgc
     1321 tcttttcggg aatgattaaa acctggtttg gtcgaagtat agttttcgta aggttattgg
     1381 cttctttgat ttcatttaca ctgatgccgg attctttgga tatgctcttt aaactgtcac
     1441 cggtttttac cgtgtattct ctggccaatg aactgttgat aaaacacaat gacaaaaaca
     1501 gggaaaatac aaaaaactgc cattttctca ttaaatacct ccttttagcg tgcggctcac
     1561 cctttgttaa aggtacacag atgtctacag gtgactcata agtgccggaa tgtaaggggt
     1621 aatttactgc aaagagaacg aagattattt gccgacaacg tgcctttaac ttcgagactg
     1681 ttacatacta aaaataaaat tatctgtcaa atttattttg caatctgtga taaaagggta
     1741 taggaaacgg cactcaaaaa tttaaagttt attgaaagct cgttaaaatt acggcaagga
     1801 tgattatgaa gctttggaaa aagatttttt ttatagcgtt gtttttaacc gtaacttttt
     1861 ttatcgttga tatagggcgc tatttttttt atcccaatgt ggcttatctg aaaaaacaca
     1921 accccgggaa aactgctttt atggaataca gggaaaaaac ctggcagcaa aaaaagttaa
     1981 agaaggaaat tagaaaaatc tgggtgccaa tttctgaagt atctccttat gtgatgaagg
     2041 ctgtaattat ctcggaagac agtaaattct ggtcgcatga aggatttgat tttgaagcaa
     2101 tgcaaaaagc gctggaaaaa gatattaaga aaaataaaat tcaagcgggc ggcagtacta
     2161 tttcccagca actggcgaag aatttatatc tttctccgga taaggacttt attagaaaac
     2221 tcaaggaagc tatacttacc tggcggatag aaaatcaatt aagcaaaaga agaattctcg
     2281 aactttattt gaacgtagtc gaatggggcg atggagtttt cggcattgaa gcagcggcgc
     2341 aaaagcatta tggcaaatcc gctgctcaat taaatgcccg tgaggcggca agactggcct
     2401 caattctacc caaccctcgc cgttacaagc cggacgatat gtcgagatat gtggaagatc
     2461 aatcggaaag aatttatcag attatggtgc gccagggcat agttattcct gaatatgatg
     2521 aggttattac agaaaaaaac ggcaatagtc actgatgaga attgtggaag aaatttgcta
     2581 atattttttt aaaaaaaatt agtaaagata ttccggctat gaaaataaaa tatgctatat
     2641 atatttgtct tattatgttt ttaatcggat gtgaagcaat tgtaaatcgg atggccttct
     2701 ttcccacggt aaccaaatcc gcggtagtgg aaaatatacc cgcgggcgtg aaagaagttt
     2761 atatgactac tgatgataat gtatgtttgt agaccgcttc gacttttttc ttataagcgc
     2821 aggaagcatt agaaacaatg cttcctgcgc tggacactag ctcgaaggtg aaatattcgc
     2881 ttttttatct gtagtggcca tagcccagaa ttcccagaat tccaatggcg atcagataaa
     2941 tagcgacaac ataattcaac atcttgggta cgattagaat aataataccg gcgataagcg
     3001 ccaacaacgg ggcaatacta agatttagag tcatgatttc atcctttctt cataaaaaag
     3061 attagaaaac tctccgaatt gtaccacgac aattccctcg acgcgggaca attccaacca
     3121 ttactattgc gcgatgtctc tttgataaaa acttgagtca agtcgcaatg aacatataaa
     3181 aactatgctt acgtgtcaag ataataagct aaactatttt taatcctgat ccttgttcaa
     3241 tattaagctg ttagcagtcc caagtttaaa c
//
LOCUS       DASVIM010000212         3772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1703826, whole genome shotgun sequence.
ACCESSION   DASVIM010000212 DASVIM010000000
VERSION     DASVIM010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703826"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /gene="dapA"
                     /locus_tag="VF305_04290"
     CDS             <1..724
                     /gene="dapA"
                     /locus_tag="VF305_04290"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX7416382.1"
                     /translation="SHEEHDRVIEITIDAVKKRVPVIAGTGSNSTAEALRLTKHAYEA
                     GADGALIVCPYYNRPTQEGLYQHFKTIAESVPISVIPYNIPSRTGVNLMPEMVAKLAK
                     IKNIVGIKEASGSIKQMSDVINLCGDDFAVLSGDDIFTLPLMVMGGAGVISVISNVAP
                     ADMAGLVDAFAAGDIARAKALHHKMSELIDALFIEVNPIPVKAALALMGKIKYEYRLP
                     LCKMAEANFEKLKKVMVNYALI"
     gene            763..1575
                     /gene="dapB"
                     /locus_tag="VF305_04295"
     CDS             763..1575
                     /gene="dapB"
                     /locus_tag="VF305_04295"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX7416383.1"
                     /translation="MVKAVVTGAGGRMGGRIISLISMIEEIKVVAAVEVAGHPIIGRD
                     VGQGLGLGRTGVSACDKLTSCIEQADVVIDFTNHEASLNYLKIAGEKNRAIVIGSTGF
                     TAVEMKKVKELAKNTRCVLAPNMSVGINVMLKVLEYCAGILNDDYDVEIVEAHHHLKK
                     DAPSGTAMQMAQVIADKLGRDLEKVGVYTRRGLIGERTKKEIGIQTIRAGDIVGEHTV
                     IFGGIGERLEFIHRAHSRDNFAKGAIRAAKWIVNQENGLYDMQDVLGLRDKK"
     gene            1592..2086
                     /gene="folK"
                     /locus_tag="VF305_04300"
     CDS             1592..2086
                     /gene="folK"
                     /locus_tag="VF305_04300"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX7416384.1"
                     /translation="MNGIICYIGIGSNLGNALQNCKCAIESLSQIEGILITRASSFYK
                     TEPVGIENQNYFINAVVEITTTLCARNLLQALQNIEKSMGRMREVKGGPRIIDLDLLF
                     YGQDVIREVDLIAPHPEIQKRRFVLEPLNEIASYFIHPAFGVSIRGLKDRLNDNKIVE
                     MIKE"
     gene            2389..3036
                     /gene="fsa"
                     /locus_tag="VF305_04305"
     CDS             2389..3036
                     /gene="fsa"
                     /locus_tag="VF305_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX7416385.1"
                     /translation="MKFFIDTANIEEIKEGLNLGMVDGVTTNPSLIAKEKKGFDVVVK
                     DILKNVKGPVSLEVVSLEAKAMFAEGKKLVQLGDNVVIKVPLCTEGLKATKMFAEAGI
                     DVNQTLIFSPLQALMAAKAGARYVSPFVGRLDDIAHDGMELVEQILTIYDNYGYETEV
                     IVASIRHPRHVLDAALMGADIATIPFKVIAQLVQHPLTEKGIETFLEDWKKVPNK"
     gene            3064..3618
                     /gene="pgsA"
                     /locus_tag="VF305_04310"
     CDS             3064..3618
                     /gene="pgsA"
                     /locus_tag="VF305_04310"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX7416386.1"
                     /translation="MIIKREIFNLPNIITLIRISVVPFLFILLSSPGEFWSLILAVIF
                     LLASFTDFIDGYVARKYQLVTTLGKFLDPIADKLIINTAMILMIPIGRIPAWIVAITI
                     MRDFLVDWIRSIASCEGIFIQASSLGKQKTFAQIIAVAALIAHYSIVGVDVHVVGIVI
                     LYLAFLLTIYSGADYFIKFYRRYL"
BASE COUNT         1127 a          657 c          868 g         1120 t
ORIGIN      
        1 ttcccatgaa gaacacgatc gggtcattga aattaccatt gatgccgtaa aaaagagagt
       61 gccggttatt gccggtacgg gatcaaacag cactgccgag gctttgcgtt tgaccaaaca
      121 tgcttatgaa gccggtgccg atggagcgct catcgtctgc ccatattata accgtcctac
      181 tcaagaggga ctttatcagc attttaaaac tattgccgaa agtgttccta tttcggtaat
      241 accatataat atacccagcc gtaccggtgt gaatctcatg cccgaaatgg tcgccaaatt
      301 ggcgaaaata aaaaatatcg tcggcataaa agaggcttcc ggctccatca agcaaatgag
      361 tgatgttatt aatctctgcg gtgatgattt tgctgtctta tcaggtgacg atatttttac
      421 tttgccgctg atggtgatgg gcggcgcagg tgttatttcc gttatctcga atgtggcacc
      481 ggcagatatg gccggattag ttgatgcctt tgctgctggc gatatagcca gggcgaaagc
      541 tctccatcat aagatgagcg agttaattga cgcgcttttt atcgaagtca atcccattcc
      601 cgttaaagcg gctttggcgc tgatgggtaa aataaagtat gaatataggt tacctctttg
      661 taaaatggcg gaggctaatt tcgaaaagct aaaaaaggtt atggtcaatt atgccctgat
      721 ttaattatct tttctcaata aataattaaa ggagatggtt gtatggttaa agccgtcgtt
      781 accggagctg gtggcagaat gggaggcaga attattagtc tcatttccat gatagaggag
      841 atcaaagtcg tagctgcggt ggaagtagca gggcatccca ttattggtcg tgacgttggc
      901 caaggactcg gcttgggtag aacaggtgtt tcagcttgcg ataaattaac tagttgcatt
      961 gagcaggccg atgttgttat tgattttaca aatcatgaag cttctctcaa ttatttaaaa
     1021 atagcgggtg aaaaaaatcg cgcgattgtg atcggttcca caggatttac tgccgtcgaa
     1081 atgaaaaaag tcaaggaatt ggcaaaaaat acacgttgtg tgttggcccc caatatgagc
     1141 gttggcataa acgttatgct gaaagtattg gaatactgtg ccgggattct taatgatgat
     1201 tatgatgtgg aaattgttga agctcatcat catttaaaaa aagacgctcc cagtggcacg
     1261 gcgatgcaaa tggctcaagt tattgccgac aaactggggc gtgatctgga aaaagtagga
     1321 gtttatacac gccgggggtt gattggtgag cggactaaaa aggaaatagg catacaaaca
     1381 attcgtgccg gtgatatcgt cggtgaacac acggtaattt ttggcggaat aggggagcgg
     1441 ctggaattta ttcatcgcgc ccacagccgc gataattttg ctaaaggcgc aattcgtgcg
     1501 gctaagtgga ttgtcaatca ggaaaatggc ttatacgata tgcaggatgt tttaggatta
     1561 agggataaaa aataaaagtg gtttttaagc tttgaatggc attatctgct atattgggat
     1621 tggttctaat ttgggcaatg ctttgcaaaa ttgtaaatgc gcaattgaaa gcctttccca
     1681 gatagaagga attctgataa caagagcttc gtctttctat aaaactgaac ctgtagggat
     1741 tgagaatcaa aattatttta taaatgccgt tgtcgagata acgacaacgc tttgtgcccg
     1801 caacctctta caggcgttac aaaacatcga aaagtccatg ggccggatga gggaagttaa
     1861 aggaggccca aggattattg atcttgatct ccttttttac gggcaggatg tgattcgaga
     1921 ggttgattta atcgctccgc atccggaaat tcaaaaacgg cgctttgttc tggaacctct
     1981 gaatgaaatc gcttcttatt ttattcatcc tgcttttggt gtttctatcc gcgggctaaa
     2041 agataggctg aacgataata aaattgtcga aatgatcaaa gaataaagag tcaaccgaca
     2101 ggagattatg tttatccgtt ttttggtatt tataatatta atttatttgc tgtataagat
     2161 atttaaaact atccggcaat taaaattggc aaaagttaat aattatcaat ttaaatcatc
     2221 tccagcgagg ggagaagact tggttgaaga tactgcttgt cacacttaca tacctttaag
     2281 tcaggcctat agaaaagaaa tatccggcaa cactcattat ttttgcagta agcgatgttg
     2341 tgaaaaatat gaattagaaa aaaatatgta aatacattag ggggagttat gaagtttttt
     2401 attgatactg ctaacatcga agaaataaaa gaaggtctta atttaggaat ggtggacggt
     2461 gttacaacca atccatcgct gattgccaaa gagaaaaaag gttttgacgt tgtcgttaaa
     2521 gatatattaa aaaatgtaaa aggaccggtg agcctggaag tcgttagcct ggaggcgaag
     2581 gccatgtttg ccgaaggcaa gaagctggta cagttgggag ataacgttgt cattaaagtg
     2641 cctctttgca ctgaaggatt aaaggcaaca aaaatgtttg ccgaggcggg tattgatgta
     2701 aatcagacct taatattttc tcctttgcag gcgttaatgg cggcaaaagc gggagcacgt
     2761 tatgtaagcc cgtttgtcgg ccgtcttgac gatattgctc acgatggtat ggaactggtg
     2821 gaacaaattc taactattta cgataactat ggttatgaaa cagaagtcat tgtagccagc
     2881 atccgccatc cacggcatgt tttagatgct gctttaatgg gcgcagatat agccacgatt
     2941 ccctttaaag taatagctca attggttcaa catccattaa cggaaaaagg gattgaaacg
     3001 tttttagaag actggaaaaa agtaccaaat aagtaaaaat aattctcact caggctataa
     3061 acaatgatca taaaacgtga aatcttcaat ctgcccaaca taataacgtt gataagaatt
     3121 agtgttgttc cgtttctttt cattttgctt tcatcccccg gagaattctg gagcctgatt
     3181 ttggcggtaa tattccttct tgcttcattt accgatttta tcgatggata tgtggccaga
     3241 aagtatcagc tggttacaac gctgggtaaa tttcttgatc ctatagccga taaactaata
     3301 attaacacag ccatgatatt gatgattccc attggccgca taccggcatg gattgtggcc
     3361 ataacaatta tgcgcgattt ccttgttgac tggatacgga gtattgcttc ctgtgaagga
     3421 atttttattc aagccagcag tttagggaag caaaagactt ttgcgcaaat catcgcggtg
     3481 gcagcgttaa tagcccatta ttcgattgtt ggcgttgatg tccacgttgt ggggatagtg
     3541 attctctatt tagcttttct gctcacgatt tattctggag ctgattattt tattaaattt
     3601 taccgccgtt atttatgaaa agaatcttca tggctagcag gattaacctt cattattaaa
     3661 agatgatttg cacataactt caatcaagct aggatattat ttgctgtggt ttcttctccc
     3721 aacaaaaata tttttaaaat actcggtgca ggtcatattt ttattgacag aa
//
LOCUS       DASVIM010000213         5744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1447897, whole genome shotgun sequence.
ACCESSION   DASVIM010000213 DASVIM010000000
VERSION     DASVIM010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447897"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VF305_04315"
     CDS             complement(<1..670)
                     /locus_tag="VF305_04315"
                     /inference="COORDINATES: protein motif:HMM:NF040068.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416387.1"
                     /translation="MSWKLKKKYNILLEQETGYVKKVWGTYNTVCLAYPNYYRTAMAN
                     LGFQTVYKIFNEQSSFLCERVFLPAPDDEAEYVSGAAEMVSLESQKSIAEFDILAFSL
                     SFENDYPHILKMLGLARIPLLSRERREDFPLVIGGGIALTLNPEPLADFFDLFILGEA
                     EEILPQFTRYFEEARRLGHDRQRLLKDLQKQIQGVYVPSLYEVKYSSGSKIKTIVSRE
                     AGLPE"
     gene            complement(735..1886)
                     /gene="ftsZ"
                     /locus_tag="VF305_04320"
     CDS             complement(735..1886)
                     /gene="ftsZ"
                     /locus_tag="VF305_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX7416388.1"
                     /translation="MFELTEVCSDNSAKIKVIGVGGGGGNAVNTMISYSLCGVDFISA
                     NTDAQALGASSSPIKIQLGMEITKGLGAGSNPEIGRQSALESRDEIRPYLEGSDMIFI
                     TAGLGGGTGTGAAPIIAEIARECSVLTVAVVTKPFQFEGKKRNIQAEEGIAQLRDAVD
                     TLIVVPNQRLLSLGGRSLSLLEAFKKADDILYQAVKGISDLILVPGLINLDFADVKNI
                     MSNMGMAIMGTGSAGGENRAVEAAQRAISSPLLEDNTIQGARGILLNITGGPDMSLYE
                     VNEASSLIQAEAHEDANIIFGTVIDENMKDEIRITVIATGFGNMVKKKESPANVAHLA
                     GFRREDLATPAFMRKEKTRDHANVVTLGINEENENIDMEIPTFLRRQAD"
     gene            complement(1902..3002)
                     /gene="ftsA"
                     /locus_tag="VF305_04325"
     CDS             complement(1902..3002)
                     /gene="ftsA"
                     /locus_tag="VF305_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX7416389.1"
                     /translation="MRKGIIVNIDSMAESIKMAVEEAEHMSGCRINSVYVGISGSHIK
                     GQNSLGIVSIKGREVDEDDVQRAIEASRAIAIPVDREIIHILPQNYVVDGQDGIKDPI
                     GMSGVRLEAKVHIVTGSTSAIQNIVKSVNRVGLDIDDIVLEQLAASEAVLSVDEKDLG
                     AVLIDIGGSNTAIAIFSEGSIKHTAILPVGGNYITSDIATGLRTPFNEAEKIKIKNGC
                     ALTSMIPKEETIRVPSVGGREDREVSRQILGRIVEPRMDEILNLAYKEIVRSGFEDLL
                     AAGVVLTGGTSLLPGISELAEQIFDMPARKGCPIGVGGLSDVVNSPVYATGVGLIVYS
                     GKQLSGDSIYRKTSSIFGNMTRTIKKWFSEFF"
     gene            complement(3176..4021)
                     /locus_tag="VF305_04330"
     CDS             complement(3176..4021)
                     /locus_tag="VF305_04330"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX7416390.1"
                     /translation="MPKRLKKSYGAKKYRIRRRFGSVLIEFLSAVGILISIVALSSLF
                     IYGYGFLLSSPYFEIKETSVRGLKELTEKDILTAAEIKPAQNLLAVNTDAVMRRVAVD
                     PWIKHVYVGRELPNRLVLEVQERNPLALIKQAGDFYLMDSEGFAFKKLGKSDEVDLPI
                     LTGSNEQDKTKSKLFLSMVDLLKTISASRQYAYLGTISEVNMDDIFGLSLLTDTGFYL
                     KFGMDGFENKLKSLNLVLADLEKRGMKNGYLCIDLCDESKITVQRRNAPGRMDTGKKG
                     KQYRI"
     gene            complement(4021..4944)
                     /gene="murB"
                     /locus_tag="VF305_04335"
     CDS             complement(4021..4944)
                     /gene="murB"
                     /locus_tag="VF305_04335"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX7416391.1"
                     /translation="MADINNIQKALGDIDAGEILYDEPMSRHASLSVGGKADALVFVE
                     NEGQMIQVVRKLKEKNMNFLPVGNLTNIIVRDGGYRGTILLMKGLKEISYLCTPEGRH
                     YISAQAGAALASVLSRALAEELTGLEFCAGIPGSVGGAVWMNAGAYGKEMKDVIETVS
                     LLDAGGEKKTLRREEISFGYRRSGLPPDIIILNAQFKLEKGERTKIKEKINEIMQGRQ
                     EKHPLDLPNAGSIFKNMPGQPAGKLIEEMGMKGANVGDAQISLKHANFIVNRGKAKAS
                     DVLALIELIQSKAKKEKGVILEPEVIIIGED"
     gene            complement(4944..>5744)
                     /locus_tag="VF305_04340"
     CDS             complement(4944..>5744)
                     /locus_tag="VF305_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX7416392.1"
                     /translation="KEAFCKFANIVPFYGCTVLCSDNLHVREIAPLIKRKMITYGIEQ
                     SADYSAQEIKFLADKTIYNLSYKGEPQGEIGLVVPGMFNVYNSLAAIAVCRELDLSLP
                     TIKKGLLGFSGVQRRLEIKGKVSDIIVVDDYGHHPTEIKETLTAARQVWKGRLIVVFQ
                     PHRFTRTKALFDEFTMAFKDADILILNDIYPASEEPIPGINSAALWAAIKQTGHPHVE
                     YIANTKETIAFLLNTARPKDTVITLGAGNIYKVGETFLKELAAKETRK"
BASE COUNT         1504 a         1461 c         1077 g         1702 t
ORIGIN      
        1 tttcaggcaa tccggcctca cgcgacacta tcgtcttgat tttgctaccg gatgaatatt
       61 ttacttcgta aagactgggg acataaacac cttgtatttg tttctgcagg tctttgagta
      121 atctctggcg atcatgacca aggcggcgtg cttcttcaaa atagcgggta aattgcggca
      181 gtatctcttc ggcctcaccc aagataaata aatcaaaaaa atcggccagc ggctcaggat
      241 tgagcgtcaa ggcgatgccg ccaccgataa ccaaaggaaa atcttcccta cgctctcttg
      301 ataaaagagg tatacgggcg agtcccaaca tttttaaaat atgagggtaa tcgttttcaa
      361 aggataaaga aaaagccaaa atgtcaaatt cggcaattga cttttggctc tccagactaa
      421 ccatttctgc cgcgccggaa acgtattcgg cttcgtcatc cggcgccggc agaaatactc
      481 tctcacaaag gaaggaggat tgctcattaa atattttata aactgtttgg aagcccaaat
      541 tcgccatagc ggtccggtaa taattaggat aggccaggca aactgtattg tatgtgcccc
      601 aaactttttt tacatagcct gtctcctgct ctaaaaggat gttatatttt tttttcagtt
      661 tccaactcat ggttttatac tggcctgtaa ccaactaaat ttaattagcc ggaaaaccac
      721 taatatactc attcctaatc agcttgcctg cgcaaaaacg taggaatctc catatcaatg
      781 ttctcgtttt cctcgtttat gcccaaggtc acaacattgg catgatcacg ggttttctcc
      841 ttgcgcataa aagcaggtgt tgctaagtct tcccgccgaa aaccagccag atgcgcaaca
      901 tttgccggtg attccttttt cttcaccata tttccaaagc ccgtggcaat gacagtaatg
      961 cgtatttcgt ctttcatatt ttcatcaatg accgtaccaa aaatgatatt ggcatcttca
     1021 tgcgcttcgg cctgaatcaa tgatgaagcc tcattaactt cataaagact catgtccggt
     1081 ccgccggtga tgttgagcaa aattccgcgc gcaccctgga tagtattgtc ttccagaaga
     1141 ggagatgaaa tcgctcgttg cgctgcttcc actgcgcggt tttcgcctcc cgcggagcca
     1201 gtgcccatta tggccatacc catattgctc atgatatttt ttacatcagc gaaatcgaga
     1261 ttgatcaggc ctggaactaa aatcaaatcg gagattccct tgaccgcctg ataaagaata
     1321 tcgtccgctt ttttgaatgc ctccaaaagc gacaaactgc ggccgcctaa actcaaaagc
     1381 cgctgattag gaacaacaat gagtgtatct acagcgtccc gcaactgagc gataccttct
     1441 tcagcctgga tgttgcgctt ctttccttca aattgaaagg gcttggtaac cacagccaca
     1501 gtcaagacac tgcattctcg ggcaatttcc gcgataatcg gcgccgcacc cgttcccgtt
     1561 cctccgccca gaccagcggt gataaaaatc atatccgagc cttccaggta cggccgaatt
     1621 tcatcgcggg attccagtgc cgattgtctg ccgatttccg gattggaacc agccccgaga
     1681 ccttttgtga tctccatacc aagctggatt ttgattggtg aactcgacgc gccgagagcc
     1741 tgcgcgtccg tatttgcgct aatgaaatca accccacaca gagagtatga gatcatagta
     1801 ttgacagcgt ttccgccgcc accgcctaca ccgatcactt tgattttcgc agaattatca
     1861 ctgcatactt ccgtcaattc aaacatgggc atcccttcct ttcaaaaaaa ttctgaaaac
     1921 cattttttta ttgttctggt catattgccg aatatactac ttgtctttct gtatatagag
     1981 tcgccggata attgcttacc gctataaacg attaaaccaa ctcctgtagc atacacaggc
     2041 gagttcacaa cgtcagataa gcctccgaca cctataggac atcctttacg cgccggcatg
     2101 tcaaagattt gctcagcgag ctcactgatg ccgggtaaaa gagaagttcc accggtcaaa
     2161 acaactccgg ctgccagtaa atcttcaaaa ccggaacgga caatttcctt gtaggcaaga
     2221 tttaaaattt catccattct cggctcgact atccgcccca atatctgacg cgatacttcg
     2281 cggtcttccc tgccgcccac actgggcact ctaattgttt cttccttagg aatcatagaa
     2341 gtcaaggcac acccgttttt aattttaatt ttttccgctt cattgaaagg agtgcgcaaa
     2401 ccggtggcaa tatcagaagt aatatagtta ccgccaacag gcagtatggc tgtatgtttg
     2461 atgcttcctt cagaaaaaat cgcaattgca gtattggacc cgccaatatc aatcaaaacc
     2521 gctcctaagt ctttttcatc aacgcttaac acagcttcac tggcagccaa ttgctccaga
     2581 acgatgtcat caatatccaa acctacgcgg ttgaccgatt ttacaatgtt ttggatagcg
     2641 gaggtggatc cggtaacaat gtgcacttta gcctccagcc ggacgcccga catccctatc
     2701 ggatctttga tgccatcctg gccgtcaact acataatttt gaggaagaat gtgtataatt
     2761 tctctgtcca cgggaatggc aatggcgcgg gaagcttcaa tcgctctttg cacatcgtct
     2821 tcatccactt ccctgccttt aatggagact atgcccaaag aattttgtcc tttaatatgg
     2881 ctgccggaaa ttcctacata aaccgaattg atgcggcatc cggacatgtg ttccgcttct
     2941 tccaccgcca tttttattga ttccgccatg ctatcgatgt tgacaatgat accttttctc
     3001 atttctttgg cgggggatgt ccctatgtga taatatcaat gcctgtttcg gtaacctcgc
     3061 caacaatggc cgtggtcttt gtcgttccta tatcgagccc aactagaaca tcgttttttc
     3121 tacccatcag tattttcacc ccttttttaa tttcactgcc cgcaggttta tcttcctata
     3181 tcctatattg tttacctttt ttgcccgtat ccatccttcc cggagcattt ctgcgttgca
     3241 ctgtaatttt tgattcatca cataaatcaa tgcacaaata accgtttttc attcctctct
     3301 tttctaaatc agccaaaact aaatttaaac ttttcagttt gttttcaaaa ccatccatac
     3361 caaacttcaa gtaaaatcct gtatctgtca gcaaggaaag tccgaatatg tcatccatgt
     3421 taacttcgga aattgtgccc aaataagcgt attgacgcga agctgatatt gttttcaaca
     3481 agtcaaccat gcttaaaaag agcttggatt tagttttgtc ttgctcattg cttccggtaa
     3541 gtatcggaag atccacttca tcgcttttac ccagtttttt aaacgcaaaa ccttcactat
     3601 ccatcagata aaaatcaccg gcctgtttga tcaacgccag cggatttctt tcctgcacct
     3661 ccagaacaag tctgttgggc agttctctgc cgacataaac atgttttatc catgggtcta
     3721 cggcaacccg gcgcataact gcatcggtat tgacggctaa cagattttgc gcaggcttta
     3781 tctctgctgc tgtaagaatg tctttttccg ttagttcctt taaaccgcgc acagaagttt
     3841 cttttatttc aaaatacgga ctgcttaaaa gaaaaccata accataaata aataaagagc
     3901 tgagagcgac tatggaaatt agtatgccca cagcgcttaa gaattcaatc agcacactgc
     3961 cgaaccgccg gcggatacgg tactttttcg ctccatagct ttttttcaat ctcttcggca
     4021 ttaatcttct ccgataatta taacttcagg ctccaaaatt acgccttttt cttttttagc
     4081 cttagactga ataagctcaa tcagcgctaa aacatcagaa gccttagcct tgcctctgtt
     4141 aactataaag ttggcgtgtt tcaatgatat ttgtgcatct cccacattcg cacctttcat
     4201 acccatctct tcaataagtt taccggctgg ctgtcccggc atatttttaa aaatagaacc
     4261 ggcattaggt aaatctaatg gatgcttttc ctggcgccct tgcataattt catttatttt
     4321 ttccttaatt tttgtgcgct cacctttttc cagtttaaat tgagcattca gtattattat
     4381 atccggcggc aagccggacc ttctgtagcc aaaagatatt tcttcccgcc ttagagtttt
     4441 cttctcgcct cctgcgtcaa gtaatgatac tgtctcgata acatccttca tttcttttcc
     4501 ataagctccg gcattcatcc atactgctcc gccaacgctg ccgggaatac ccgcgcaaaa
     4561 ttcgagacca gtcagttctt ccgccagcgc gcggctgagt actgaagcca gcgcggcgcc
     4621 ggcctgagct gaaatataat gccggccctc gggcgtacaa agataactga tctctttcag
     4681 gcctttcata agtaaaattg ttccacggta accgccatca cgaacaatta tattcgtcaa
     4741 gtttcccacc ggcagaaaat tcatattctt ttctttaagt ttccttacaa cctgaatcat
     4801 ttgaccttca ttttctacaa atactaaggc gtctgctttg ccgcccacag aaagcgacgc
     4861 gtggcgagac atgggttcat catataaaat ttcccccgcg tcaatatcgc ccagagcttt
     4921 ctgaatgtta ttaatatccg ccattatttt cttgtttcct ttgccgctaa ttcctttaaa
     4981 aaagtttccc ctactttata aatattacct gcgcccaatg taataactgt atctttcggt
     5041 cttgccgtgt ttaaaagaaa tgctattgtt tcttttgtgt tcgcgatata ttccacgtgt
     5101 ggatgacccg tttgtttgat tgccgcccat aacgcggcgg agttaattcc gggaataggt
     5161 tcttcactgg ctggatatat atcgttcaga atcagaatgt ccgcgtcctt aaacgccata
     5221 gtgaattcat caaaaagagc tttggttctt gtaaaacgat gcggctgaaa aacgacaatt
     5281 agcctaccct tccaaacctg ccgggcagcg gtcagtgtct cttttatttc cgtcggatgg
     5341 tgtccgtaat catcgacaac aattatatcc gatacctttc cttttatttc cagacgtctc
     5401 tgcacaccgg aaaagccaag caatcctttt ttgatggtcg gcaaactcaa atccaattct
     5461 cgacataccg caattgctgc cagggaatta tatacattga acatccccgg cacgaccaag
     5521 ccgatttctc cctgaggctc tcctttataa ctaagattgt aaatagtttt gtctgccaga
     5581 aattttatct cctgggcact gtagtcagca gactgctcaa tgccataagt tatcatcttg
     5641 cgtttgatca acggagcaat ttcccgcaca tgcagattgt cgctgcaaag gaccgtgcaa
     5701 ccataaaagg gaacaatatt ggcaaacttg caaaacgcct cttt
//
LOCUS       DASVIM010000214         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_509274, whole genome shotgun sequence.
ACCESSION   DASVIM010000214 DASVIM010000000
VERSION     DASVIM010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509274"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VF305_04345"
     CDS             <1..680
                     /locus_tag="VF305_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7416393.1"
                     /translation="CGADNFSYLIYGKTQALAIDGGAWEDILSFLDHHSLVLAVVTNT
                     HNHFDHTSGNDHLFKATRAQFLNFTDLTDNKKIHIDGETICVYRTPGHTDDSVCFHTG
                     DILITGDTLFNGTIGNCFSGDQKSFYLSIKRLMALPDETKIYAGHDYVKTSLAFARHL
                     EPENKDIERFWNSYDPDFVHSTMADERLINPYLRFNEKPIVNLLKENNLPYATEWERW
                     ESLMSIE"
     gene            899..2551
                     /locus_tag="VF305_04350"
     CDS             899..2551
                     /locus_tag="VF305_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX7416394.1"
                     /translation="MGNALLKPIKLGIYELPNRIFMAPMTRSRADNPDNAATPLIAEY
                     YAQRASAGLIISEGSQISQQAVGTINTPGIYTPAQIEGWKLATRAVHDKNGYIFCQLW
                     HVGRMSHPDFHSGKLPVAPSAINPQDKVFTPNGFKDTVKPQALTVTEIHDITKDFRKA
                     AENAITAGFDGVEIHSANGYLFHQFFSNCSNIRKDEYGGSRENKARFFFETLVVVGQA
                     IGFDKVGVRLNPSAHGFFGITIDKDTIPTFEYIVERLNDYHNLAYAHFTEPFLRVDDV
                     PYAVKEIAKHFRPRYKGKMIINSGFRYGSGIRVIEEGYADAVAFGKPFISNPDLVERI
                     RSIVDWARWDDQTFYTTGPKGYTDYPPISGMSQSHAQSSAPDANNDFKIDQPSVSLTT
                     PNEDEKTARIKVGDKFGDFSLKNHKDAVIDTAALTGKKILLSFHPLAWTDVCARQMQS
                     LESNFESFQNLNTVPLGLSVDSVPCKKAWAEKLGIEKTDLLADFWPHGGLALRLGIFI
                     DRFGFSERANIIFDKDRRAIFVKIYPLKELPDVKELLEFLKK"
     gene            2805..>3406
                     /locus_tag="VF305_04355"
     CDS             2805..>3406
                     /locus_tag="VF305_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7416395.1"
                     /translation="MIRSALLVTLGVAITAFISFWCVIFSIFPNADNKIHKVANLWGK
                     ILLLICNTKVEVIGKENILHSKPQIFVANHQSDFDILIALAYIPVQFRWIAKKEIFNI
                     PVFGAAMRSAGYIEIDRQNREKALQSLDEAALRIREGKSVMTFPEGTRSRSGEIKPFK
                     QGTFHLAIKSGVSIVPVSIIGSGQIMPKRSLKISSGKVKLV"
BASE COUNT         1004 a          786 c          697 g          919 t
ORIGIN      
        1 gatgtggcgc tgataatttt tcctatctca tctatggaaa aacacaggcg ctggctatag
       61 acggcggagc atgggaggac atcctctctt ttcttgacca tcatagtctt gtgctagcgg
      121 tcgtaaccaa tacgcataat cactttgacc atacatcggg caacgaccac ctattcaagg
      181 cgacacgcgc acaattctta aattttactg atcttaccga taataaaaaa attcatatcg
      241 atggggaaac aatttgtgtt taccggactc ccgggcatac ggacgactcc gtttgttttc
      301 acacaggcga tatcctgatc accggcgaca ccctcttcaa cggaaccatc ggcaactgtt
      361 tttccggcga ccagaagagt ttctatctgt ctataaaacg cctgatggcc ttgccggatg
      421 aaacgaaaat ttatgccggg catgactacg tcaaaacctc cctggctttc gccaggcatc
      481 tggaaccgga aaataaggat atcgaacgct tctggaactc ctacgatccc gattttgtcc
      541 attccactat ggccgatgaa cgcctgatca atccctatct gcgatttaac gaaaagccta
      601 tcgtaaatct cctcaaagaa aataatcttc cctacgccac ggaatgggaa cgatgggagt
      661 cgttgatgtc tattgaataa tctgcccgta tttttttaat aagtatataa tatcccaaac
      721 attaagacga acgattttat tgattatctt gacataagac agtcatcatc gttatattaa
      781 attaaataat agactattgc ttatttaaca aaagtgattt ttaacaggag accggctccg
      841 gcacaagtaa atgccccaca taacatcttg aaatgaattg taaataagga gaaagataat
      901 gggaaatgca cttttgaaac cgataaagct gggaatatac gaacttccca accggatatt
      961 tatggctcct atgactcgaa gccgtgccga taatcccgat aatgccgcaa caccgctgat
     1021 tgctgaatac tatgcccaac gcgcctccgc cggtctgatc atcagtgaag gctcccagat
     1081 ttctcaacag gcagtgggca ccatcaatac gccggggatt tacaccccgg cacaaattga
     1141 aggctggaag ctggccactc gggccgtcca cgataaaaac ggttacatct tctgtcaact
     1201 ctggcatgta ggtcgcatgt ctcatcccga ttttcatagc ggcaagcttc ctgtggctcc
     1261 atcggcaata aatccccagg ataaggtttt cacccccaat ggtttcaaag atacggtaaa
     1321 accacaggcc cttactgtta ctgaaatcca tgatattacc aaagatttca gaaaggctgc
     1381 cgaaaacgcc atcactgccg ggtttgacgg agtggagatt cattccgcca acgggtatct
     1441 ttttcaccag ttcttctcca actgctccaa tatccggaag gatgaatacg gcggctctcg
     1501 cgaaaataag gcacgttttt tcttcgaaac attagttgtt gtcggtcagg ctatcggttt
     1561 cgataaagta ggtgtccgcc taaatccctc cgcccacggt ttttttggta ttaccatcga
     1621 taaggatacc attcccacct ttgaatatat tgtcgagcga ttaaacgatt accataattt
     1681 agcctacgcc catttcactg agccctttct acgggtggat gacgttccct atgccgtaaa
     1741 ggaaatcgct aagcatttcc gcccgcgcta taagggtaaa atgattataa acagcggttt
     1801 ccggtatgga agcggtatcc gtgttattga agaaggttat gccgatgctg tcgctttcgg
     1861 caagccattc atctccaatc ctgaccttgt ggaacgcatc cgcagcatag ttgattgggc
     1921 tagatgggat gatcagacct tttacacaac cggacccaaa ggttatacgg attacccccc
     1981 tatttctgga atgagccaat ctcacgcaca aagctctgcg ccggacgcca acaatgactt
     2041 caagatagac caaccatcag tttcattgac aactccgaat gaggatgaga aaacggccag
     2101 gataaaagtc ggagataaat ttggagattt ttctttaaag aaccacaaag acgcggttat
     2161 cgatacggcg gcattaacag gcaaaaaaat acttctttca tttcatcccc ttgcttggac
     2221 agatgtctgc gcccggcaaa tgcagtcact ggaaagtaat tttgaaagct tccaaaattt
     2281 aaataccgtt cccttggggc tttctgttga ttccgtgccc tgcaaaaagg cctgggcgga
     2341 aaaactcggc atcgaaaaaa cggatttatt ggccgatttt tggccgcacg gaggccttgc
     2401 cttgcgtctg gggatattta tcgaccggtt cggtttttcc gagcgagcca acataatttt
     2461 cgataaagac agaagagcga tattcgtcaa aatttatcct ttgaaggaat taccggatgt
     2521 aaaagaactt ctggagttcc tgaaaaaata ggtcttttag cgttttgaaa ataccgggtt
     2581 catttcaact aacgaataaa aaaacaacta aattttttat tttttttgac cggaccatag
     2641 acctcttaaa aaaatcaatg cctacaggcc tttatcatca tctgcgcgtc attacagcat
     2701 aaaggtaaaa atattaataa gtttttatat ataattcatt attgacttca gccacattat
     2761 caagtaaaaa ttacaaatca atcagcctga aggaaaggac gttcatgatc cgttcggcgc
     2821 ttttagttac attaggtgtt gccataaccg catttatctc cttttggtgt gtgatttttt
     2881 caatttttcc caatgccgac aacaaaattc ataaggtagc caatctctgg ggtaaaattt
     2941 tacttttaat atgcaataca aaagtggaag taatcggcaa ggaaaatatt ctgcacagca
     3001 aaccgcaaat attcgtggcc aatcaccaaa gcgatttcga tattttaatt gccttagcat
     3061 acattcccgt tcaattccgc tggattgcca aaaaagagat atttaatatc cccgtatttg
     3121 gcgcggcgat gagaagtgcc ggatatattg aaattgaccg gcagaatcgt gaaaaagccc
     3181 tgcaaagcct tgatgaagcg gcactgcgca tccgcgaagg aaaatcagtc atgacttttc
     3241 cagaaggaac aagaagccgc agtggagaaa taaaaccttt taagcaagga acctttcatc
     3301 tcgccattaa atctggtgtg tccattgtgc ctgtttctat tatcggcagc ggccagatca
     3361 tgcccaaaag gtccctaaaa ataagttccg gcaaagtgaa attggt
//
LOCUS       DASVIM010000215         4259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_510234, whole genome shotgun sequence.
ACCESSION   DASVIM010000215 DASVIM010000000
VERSION     DASVIM010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510234"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..2701
                     /locus_tag="VF305_04360"
     CDS             194..2701
                     /locus_tag="VF305_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX7416396.1"
                     /translation="MRYSLQNKIILTLLILFLVGISGCSSMLGYMPAPPDSMVKIELK
                     DHYDVDIPKQYVNQLKWASVMSDWAIPYIFMNVGLHFESVDDELRSIHFFDRAITEFR
                     KRKDVYGEGTVTSRKVIAFYKFGKIQDAYNAIKEAEKNWPDAPLNAFVFQNYGHYYLM
                     NGDYNKALKHFRQSFKSNQNFRDDFNLLMLRRDTELEYGMAVFLAGYIPAKSPKLTLS
                     DFGDVIFHLNQVIVLDKEIGKTKIDQFTPDIVFHTIESNVYNFLGLAYIINGQLPEAL
                     KNLDTSIKLARKADFCQGEIKSIFFRNQVYLLEKNITEGQKAAQQLNEIADKYHLPFY
                     QIWAKFILSRYYQGFGDTSKAINLLKDAMAIMEKQRGELAINMLKEIFPFNWQALYEA
                     LIELLAEEGDYKGALETAERAKAWGIVDLLAGKNIGKNSAEAELIKQDKNYINEMADG
                     YKKVLAAAGDDFAWKKALDKMEKAENAHRDVIIKIKAQNEELYSLLFVESLNTDEMRQ
                     LLDKNTTLFSYYITDKVLYIWAMNKDRVHLERIKITREEVAKLISSFIAAITTKDKNQ
                     AESLSEKVYSIFLKPVIPFVSGDRIGFIPHGPLHYLPFAAMSYKGQYLVEGFSIFYLP
                     NAGVLKYVLKKKPAQGTNVLAFANPDLGDKQLDLPYAEAEVESIRKIIPQAIIFRREE
                     VTETKAKAMIGSYDIIHFAVHGLFVEDAPIKSGLLLTPAAQDDGYLTAAEIFKLQFRG
                     RMVVISACKKASGISSTGEGIVALNRSFLYAGSPSVVTTIWNVDDNSTATFMDIFYRN
                     LEKNESIADSLRVTQNEMIRLGYTPFDWAAFILTGKY"
     gene            2811..3860
                     /gene="rfbB"
                     /locus_tag="VF305_04365"
     CDS             2811..3860
                     /gene="rfbB"
                     /locus_tag="VF305_04365"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX7416397.1"
                     /translation="MNLMVTGGCGFIGSNFIRYLIGNPGFSGRIINVDKLTYAGNPEN
                     LVGIASDFAPRYIFQKVDICDAAKLEEVFLQYDVDAICHFAAESHVDRSIKNPENFIQ
                     TNIIGTFNLLEIAKARGDKFKLFHHVSTDEVYGSLGAAGFFSEDAPYRPNSPYSASKA
                     ASDHLVRAYYKTFGMPATISNCSNNYGPYQFPEKLIPLIVLHALEGKPLPVYGDGKNV
                     RDWLYVEDHCDAIWRIMTSGRTGETYNVGGNAEMENILIVQMICDILDEIKPAADRKP
                     RRRLIAFVRDRPGHDRRYAIDFAKLSRELGWSPQESFNSGLRKTLNWYLNQKKWVENI
                     KTGAYQTWIKEQYGS"
     gene            4076..>4259
                     /locus_tag="VF305_04370"
     CDS             4076..>4259
                     /locus_tag="VF305_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416398.1"
                     /translation="MRKKLQGKNFSSSKSYLMESEDEAVRLDIKTDPEALCKQAIWCG
                     VKPGMRILDGGCGPGKT"
BASE COUNT         1308 a          809 c          940 g         1202 t
ORIGIN      
        1 gtttaaaaaa catatgtttt cgggcaattt ttcaaatttt ttagaaagat aaaattattt
       61 tccttatgtt agaaaaatat tttagattat acctctctat ttttaaatgt tttaaattaa
      121 tagttgaaaa agacttgcaa gtaaaccgat aaaagtatta aaaagatcaa ataaatattg
      181 ataagatgag cctatgagat attcattaca aaataaaatt atcttaactc tcttaatatt
      241 atttttggtt ggaatttccg gctgcagcag catgctcggg tatatgcctg cgccgccgga
      301 ttcaatggtt aaaatcgaac taaaagacca ttatgatgtc gatataccta aacaatatgt
      361 caatcagctc aaatgggcga gcgtcatgtc ggattgggct attccctata tttttatgaa
      421 tgtgggactg cattttgaat ccgttgacga tgaattgcgc agcatccatt tttttgaccg
      481 ggctataact gaattccgca aacgcaaaga cgtctacgga gaagggacag taaccagccg
      541 gaaagtaatt gctttctata aattcggcaa aattcaggat gcttataacg ccattaaaga
      601 agcggagaaa aactggcctg acgctccgct caatgctttc gttttccaaa attacggcca
      661 ttattatttg atgaatggtg attataataa agcgctcaaa catttccggc agtcttttaa
      721 gtccaaccag aactttcgcg atgattttaa tttgctcatg ttgcggcgcg acactgaatt
      781 ggaatatgga atggctgtat ttctggccgg gtacattccg gcaaagtccc caaaattgac
      841 gttgtcggat tttggcgacg tcatcttcca tttaaatcag gttattgttt tagacaaaga
      901 aattggaaag acaaaaatcg accagtttac tccggatatt gttttccaca ctatagaatc
      961 caatgtatat aactttttag gcttggccta tattatcaat ggccagcttc ccgaggcgtt
     1021 aaagaacctc gacacctcca taaaattagc caggaaagct gatttttgcc aaggtgaaat
     1081 aaaaagtatt ttctttcgca atcaagttta cctgctggag aaaaatataa ccgaagggca
     1141 aaaggctgcc cagcagttaa atgaaattgc tgataaatac catcttcctt tttaccagat
     1201 ttgggcaaag tttattctat ctcgctatta tcagggattt ggtgacacgt ctaaagctat
     1261 aaatttgctc aaggacgcca tggccatcat ggaaaagcag cgcggcgaat tggcaattaa
     1321 catgctcaaa gaaatattcc cgtttaactg gcaggcgctc tacgaagccc taatcgaact
     1381 tttagcggaa gaaggtgatt acaaaggtgc gttggaaacg gcggaaagag caaaagcgtg
     1441 ggggatagtt gatttgctgg cgggaaaaaa cattggaaaa aattccgccg aagccgaatt
     1501 aatcaagcag gataaaaatt atatcaatga aatggcggat ggctacaaaa aagtgctggc
     1561 ggcagccggg gacgattttg cctggaaaaa ggcgctggat aaaatggaaa aggccgagaa
     1621 tgcgcatcgc gatgtaatta tcaaaatcaa agcgcaaaac gaagaacttt attcgctgct
     1681 ttttgttgag tcgctgaaca cagacgaaat gcgtcaattg ctggacaaaa atacaactct
     1741 ctttagctat tatattacag ataaagtgct ttatatttgg gcaatgaaca aagatcgagt
     1801 gcatctggaa agaatcaaaa tcaccaggga agaagtggct aaactgattt cttcttttat
     1861 tgcggcaatt accaccaaag ataagaatca agccgagtct ttatccgaaa aggtttacag
     1921 tatcttcctg aaaccggtga ttccttttgt gtcgggcgac cggatcggct tcattccgca
     1981 cggccctctt cattatttgc cttttgcggc aatgagctat aagggacaat atctcgtaga
     2041 gggcttttcc atattttact tgccgaacgc aggcgtgcta aaatatgtgc tgaagaaaaa
     2101 accggcacaa ggcacaaatg tgttggcctt tgctaatcct gatttgggcg acaaacaact
     2161 tgatttgcct tacgccgagg cagaagtgga aagtattaga aaaatcattc cgcaggcaat
     2221 tatttttcgc agggaagaag tgacggaaac caaagcgaaa gcaatgatcg gcagctatga
     2281 tataatccac ttcgctgtgc atggcctttt tgtcgaagat gcgcccataa aatctggttt
     2341 gttgttgact cctgccgcgc aagatgacgg ttatctcact gctgccgaaa tatttaagct
     2401 tcaatttaga ggacgcatgg tggtgataag cgcctgtaaa aaggcttcgg gaatttcttc
     2461 cacaggcgaa gggatcgtgg cgctcaacag atcttttctc tatgcgggca gtccatccgt
     2521 tgtgacgacc atctggaacg ttgacgacaa ttccacggcg acctttatgg atatttttta
     2581 tagaaatctg gaaaaaaatg aaagtattgc cgattcatta agagtaaccc aaaatgaaat
     2641 gatccgcctt ggctacacgc cctttgattg ggcggcattt atattaacag gcaaatattg
     2701 atttctattg agcatgataa aatatttgct tgagatttcg ctatagaata aagaaaaggg
     2761 aaaagtatta tttaaagatg aagacaaaaa acaaaagcaa ggttggattc atgaatttaa
     2821 tggtaacagg cggttgtgga tttatcggca gtaattttat ccgctattta atcggcaatc
     2881 cgggcttttc cggcagaatc atcaatgtgg acaaattaac ttatgcgggg aatccggaaa
     2941 acttggttgg cattgcctct gattttgcgc caagatatat tttccaaaag gtcgatattt
     3001 gtgatgccgc caaactcgaa gaagtatttc tccaatatga tgttgatgcc atttgccatt
     3061 ttgccgctga atcgcatgtt gaccgctcga taaaaaatcc ggaaaatttt atccaaacaa
     3121 acataatcgg aacttttaac cttttggaaa tagctaaagc ccgcggggat aaatttaagc
     3181 tctttcacca tgtcagcacc gacgaggttt acggcagtct cggagccgca ggctttttta
     3241 gcgaagatgc gccgtatcgt cccaatagcc cttattcggc atccaaggcg gcctccgatc
     3301 atttggtgcg cgcttattat aaaacttttg gaatgcctgc gacaatttcc aattgttcta
     3361 ataattatgg cccttatcag tttccggaaa agctcattcc attgattgtt ctccatgcac
     3421 tggaggggaa gccgctgccg gtttatggcg acgggaaaaa tgttcgggat tggctttatg
     3481 tcgaagacca ttgcgacgcc atctggagaa ttatgacttc cggaaggacc ggagaaacat
     3541 acaatgtggg cggcaatgcg gaaatggaaa atattttaat cgtgcaaatg atttgtgata
     3601 ttctggatga gatcaaaccc gcagcggata gaaagccgcg gcgccggttg attgctttcg
     3661 ttcgggatcg tcccggccat gatcgtcgtt acgctattga ttttgccaaa ttaagtagag
     3721 aactgggttg gtctccacag gaatcattta attccggttt gcgaaaaact ctgaattggt
     3781 atttgaacca aaaaaagtgg gtggagaata ttaaaaccgg cgcttatcag acatggatta
     3841 aagaacaata cggctcttga gaaaaagccg ttacccatga tttaccaccg ttactttctg
     3901 caccataacc taaaaaaagc ttcaaatcac ttgtttttat ttaattaatt tgctaaaaga
     3961 agcgaagact cgctaaagca aaggaaaaga gctgttgtga aagttgctat atttagtcac
     4021 tcataattca tcgctgttga ttattaatgt tttattcttt ctataggagt tgctaatgcg
     4081 aaaaaagctg caagggaaaa acttttcctc atccaaatca tatctcatgg aaagtgaaga
     4141 cgaagctgta cggctagaca ttaaaaccga tccggaggct ttgtgtaagc aggctatttg
     4201 gtgcggagtg aagcccggga tgcgtatttt ggatggcggc tgtggtccgg gaaaaacaa
//
LOCUS       DASVIM010000216         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_765118, whole genome shotgun sequence.
ACCESSION   DASVIM010000216 DASVIM010000000
VERSION     DASVIM010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765118"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /gene="purM"
                     /locus_tag="VF305_04375"
     CDS             <1..981
                     /gene="purM"
                     /locus_tag="VF305_04375"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX7416399.1"
                     /translation="IKPLIKTTARKEVISGIGGFGGLFHLDSAKLKSPIFVSSTDGVG
                     TKLRIAQLVDKHDTIGIDLVAMSVNDVVVQGAEPLFFLDYLATGKIELEKSVQIIEGI
                     IQGCNQAGCTLLGGETAEMPGSYKTGEYDLAGFCVGVVESDKLIDGSSISVNDRVIGL
                     ASSGLHSNGFSLARKVLLGKGKLGINDTVAGLNKTIGLELLEPTRIYVKSLLNLFKSF
                     NIKGSVHITGGGFYDNIPRIIPNSCRCVINKKSWDVPPIFNVIREMGNIDEKEMFRVF
                     NMGIGMMIIVAEKESQEVLDQLEMLALGEKAYLIGSVEKREDNQDQVCFY"
     gene            1001..1666
                     /gene="purN"
                     /locus_tag="VF305_04380"
     CDS             1001..1666
                     /gene="purN"
                     /locus_tag="VF305_04380"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX7416400.1"
                     /translation="MVDLLKLGVLISGSGSNLQSIIDHIERGSLKAVIKIVISNNPDA
                     YGITRAIKHGISVVALKQEDFKTKQDFDLELIRILKSNDVELVILAGFMRILTSAFLK
                     AFPQKIMNIHPALLPSFPGTHGQKQALEYGVKFSGCTVHFVDEGVDTGPIIIQGVVPV
                     NDDDTEETLTARILKEEHRIYPRAIQLFAEGKIEIKGRKVKIKAAEKTSFHSLDNPSL
                     TNF"
     gene            1715..>2211
                     /locus_tag="VF305_04385"
     CDS             1715..>2211
                     /locus_tag="VF305_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7416401.1"
                     /translation="MMYDLIVIGEDLSSHVAAAVASHCGLNTALVAESGIGGKCLIGD
                     FAFNIDPTPLTGFGANQTCLSLLAQLDIPPIEGESLLLNPAYQIILPEHRLDFFSDKE
                     SLVKEMVREFPELAVEINSFYDAVVKNSDIFGKWLHDHPLIQPRSIKDYYNYFKLMPY
                     LIKYK"
BASE COUNT          674 a          412 c          475 g          650 t
ORIGIN      
        1 attaagcctt taattaaaac aactgcacgc aaagaagtta tttcgggaat cggcggtttt
       61 ggcgggcttt tccatctgga ttcggcaaaa ctaaaaagtc cgatatttgt ttcttccacc
      121 gacggtgtgg gcacgaaact gcgaattgct caattagtag ataagcatga tactatcggg
      181 attgatcttg tagcgatgtc ggtcaatgac gttgttgttc agggcgctga gccattattt
      241 tttctcgatt atcttgccac aggtaaaatc gaattagaaa aaagcgtgca aattatcgag
      301 ggcattattc aaggttgcaa tcaagccggc tgcactcttc tgggcggaga aaccgctgaa
      361 atgcccggtt cttataaaac cggtgaatac gatcttgccg gcttttgtgt cggtgtagtg
      421 gaatccgata aactaattga cggctcttca atcagcgtca atgaccgggt gatcgggctt
      481 gcctccagcg gcttgcacag taacggattt tctttagccc gaaaagttct gctgggaaaa
      541 ggcaaattag gtattaacga cacagttgcc ggtttgaaca aaacaatcgg gctggaactt
      601 ttagaaccga cacgaatcta tgttaaatct cttcttaatc tttttaaaag ctttaacatt
      661 aaaggcagtg tccacattac cggaggtgga ttttacgaca atattccgcg aataattcct
      721 aattcctgcc ggtgcgtgat taacaaaaag agttgggatg taccgcccat ttttaatgtt
      781 attcgggaaa tgggtaatat cgacgaaaaa gaaatgtttc gtgtttttaa catgggcata
      841 ggaatgatga tcatcgttgc ggaaaaagaa tctcaggaag ttcttgatca gttggaaatg
      901 ctggcgctgg gtgaaaaggc ctacttaatt ggctctgtag aaaaaagaga agataatcag
      961 gatcaagttt gtttttatta aaattaggat caaataattc atggttgatt tattgaaatt
     1021 gggagtgtta atctccggca gcggctccaa tctgcagtca attattgatc atattgagag
     1081 aggttcatta aaagcggtta tcaaaattgt tatcagtaac aatcccgatg cttacggaat
     1141 caccagagca ataaagcacg gcatttcagt tgttgcctta aaacaagaag actttaaaac
     1201 caaacaagac tttgatttag aattaataag gattttaaaa agcaatgacg tagagttggt
     1261 tattttagcc ggcttcatgc gtattcttac ttcagccttt ctgaaagctt tcccccaaaa
     1321 aataatgaat atccaccctg ctctgctgcc atcttttccc ggaacacacg gacagaaaca
     1381 ggctttggaa tacggagtca aattctccgg ctgcacagtc cattttgttg atgaaggtgt
     1441 tgatacagga ccgatcatta ttcagggtgt tgtacccgtg aacgatgacg atacagaaga
     1501 aactttaacg gcaagaatat tgaaagaaga acatcggatt tatccgagag cgattcagct
     1561 ttttgctgaa ggcaaaattg aaataaaagg acgtaaggta aagattaaag ctgcagaaaa
     1621 aacatctttc cattctttag ataacccgtc tttaactaac ttttgagaat tatgaatatt
     1681 gcagcagatg tttgcttatg aggaaataaa ttaattgatg tacgatctaa ttgtcatagg
     1741 tgaagatctt tcttcccatg ttgccgcggc agttgcttcc cactgtggcc ttaatacagc
     1801 gctggtagcg gaaagcggca ttggcggaaa atgtttgatt ggtgattttg cttttaacat
     1861 tgatcccaca cctttaaccg gttttggcgc aaatcaaaca tgtctttccc ttttagccca
     1921 actggatatc cctccaatcg aaggggaaag ccttttattg aatcctgctt accagataat
     1981 actacctgaa caccggctgg attttttcag cgataaagaa tcactggtca aagaaatggt
     2041 tagagaattt ccagaactag ctgtggaaat aaattccttt tatgatgctg ttgtaaaaaa
     2101 ctcggacatc tttggtaaat ggctgcatga tcatcccttg atccagccca gaagcataaa
     2161 agattactac aactacttta agctaatgcc ttatttaata aaatataagt t
//
LOCUS       DASVIM010000217         3462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Smithellaceae bacterium isolate SMAG_U2993
            k141_1023591, whole genome shotgun sequence.
ACCESSION   DASVIM010000217 DASVIM010000000
VERSION     DASVIM010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959625
            Sequence Read Archive: SRR10489818
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithellaceae bacterium (soil metagenome)
  ORGANISM  Smithellaceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 881
            CDSs (total)                      :: 860
            Genes (coding)                    :: 855
            CDSs (with protein)               :: 855
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Smithellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023591"
                     /isolate="SMAG_U2993"
                     /isolation_source="soil"
                     /db_xref="taxon:2964540"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            472..1620
                     /locus_tag="VF305_04390"
     CDS             472..1620
                     /locus_tag="VF305_04390"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7416402.1"
                     /translation="MQIQRIAEIETLKRLVKNNPVVAILGPRQCGKTTLSHQFSSQWP
                     YAATFFDLENPRDVQRLDEPLLALENTGGLVVIDEIQRAPDLFPVLRVLADRNKKTKY
                     LILGSASRDLIRQSSETLAGRISYMEIGGFSSQLVGVSKTEKLWIRGSFPRSFLATSE
                     ASSYKWRLDFISTFLERDIPQLGINIPAKSLGRFWKMLAHYHGQVFNASEIGRSLGVS
                     DHTAQRYLDLLSGTFMVRQLRPWYSNTKKRIVKRPKIYFRDSGILHALFALENKNDVL
                     LHPKLGSSWEGFALEEAVKLAQLREDEVFFWSVHAAAEIDLVFQKKGRLYGIEVKYAQ
                     APNITQSMRSAMAELSLSHLWIIYPGRESYPLDRHVTVISMADLNKIA"
     gene            1821..2183
                     /locus_tag="VF305_04395"
     CDS             1821..2183
                     /locus_tag="VF305_04395"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7416403.1"
                     /translation="MESILKLQADICKIFANDKRLEMINLLKDKEMSNSELMQKTGLS
                     KVTMTQHMNVLKSKGVIAVRREGVQLYYRIANPKIMQACNLMKEVLIEQLQEKEKMAS
                     RLVKASNKLKARIASRKE"
     gene            2341..2760
                     /locus_tag="VF305_04400"
     CDS             2341..2760
                     /locus_tag="VF305_04400"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7416404.1"
                     /translation="MSKSLMEYFNKQPRLGVLSTSNKAGEVNSAYFGSPRMIDESTMI
                     VALGKNKTLANLQENPNACYMIMEPGKSIFEWKGVRIYLKMTRCETSGDNVDMMKKAI
                     SEKAGEAAAKMIHAAVTFSVASARPLADFNQGWEKSI"
     gene            2835..3089
                     /locus_tag="VF305_04405"
     CDS             2835..3089
                     /locus_tag="VF305_04405"
                     /inference="COORDINATES: protein motif:HMM:NF037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HEX7416405.1"
                     /translation="MGIADIIGFTAAVICSLAMIPQVVKIYQTKETRDLSLGAFAALG
                     TGLFLWLVYGLLIHSVPVIFGNAVGLSLVLYVIIMKLIHG"
BASE COUNT         1025 a          701 c          793 g          943 t
ORIGIN      
        1 gaaattaatt gcctgtatat ttggagcgta atcggggacg gttctattga aattccttat
       61 cagtcctggt tgtgcggtgg tggaaagcag aagaattgag cccgcaatcc cgattagctt
      121 cgcaacggga tcaattccca atagcctttg gctattggat accgctgtat gctttgcata
      181 caggataatt ccacttgcgc ttcgaacttc gctatgctcg ttcttagcga gtgtcattaa
      241 ttcgactaac aaactttaat acgccaatag tgacctttag gttattagct aagtttctga
      301 aattttggac tcactacttt gctatgctcg ttttaccacg gcgttacacg cctggtataa
      361 ttcgtccagc aaacttcagt gcactttgtt tctgaagctt gagtctcatt atcagcgagt
      421 ctcattgaaa aatcgacttg acatatcgat atttcatggt agtctctagc catgcaaatt
      481 caaagaatcg cagagattga aacattaaaa aggcttgtta agaataatcc tgttgttgcc
      541 attttaggtc ccaggcagtg cggcaagaca acgctatccc atcagttttc ttcgcaatgg
      601 ccttatgcgg cgactttctt cgatctggaa aacccacggg atgttcagcg gctcgacgaa
      661 ccactgctgg cgctggaaaa caccggtggt ctggtcgtga tcgatgaaat ccaaagagca
      721 cctgatttgt ttcccgtgct tcgcgtgctt gccgaccgca acaagaagac taaatatctc
      781 attctgggaa gtgcatcacg tgatctaatc agacaatcat cggaaacact ggctggtcgc
      841 atcagttata tggaaatagg cggtttttct tcgcagctcg tgggtgtgtc aaaaacggaa
      901 aagttgtgga tcaggggttc tttccccagg tcttttttag cgacgagtga agcatcctct
      961 tataaatggc gactggattt tatttccaca tttttagaac gggatatacc gcagttggga
     1021 attaatattc ctgccaaatc attaggccgc ttctggaaaa tgcttgcgca ttatcatggt
     1081 caggtattca acgcctctga aatcggcagg agccttggtg tttccgatca tactgcgcaa
     1141 cgatatctgg atttactctc cggaaccttt atggtccgtc aattacggcc atggtattcc
     1201 aatacaaaaa agagaattgt caaaagaccg aaaatttatt ttcgtgattc cggaatactg
     1261 catgccttat tcgcattaga aaataaaaat gatgttctgt tgcaccctaa actcggctct
     1321 tcctgggaag gttttgcact ggaagaggcg gtaaaattag cgcagctcag agaagatgag
     1381 gttttcttct ggagtgttca tgcagccgcc gagattgatc tggtatttca aaaaaaaggg
     1441 cggctctacg gaattgaggt taaatacgcg caggcgccga acattacgca gtccatgcgt
     1501 tccgccatgg ctgagttgtc actgagccat ctatggatca tttacccggg cagagaatct
     1561 tatccactgg atcgccatgt tacggtgatc tcaatggcgg atttaaataa gatagcgtga
     1621 aatgaatgac actggtctct ttggattagt tatcagtcac gactgtgctg tgatggaaag
     1681 gagggaattg aactcgcaat cccgataagc tacgcttacg ggatcaattc gactttgaaa
     1741 tttttctgca ctttgtttga aaaattttag tctcattgaa aataatgggg ataagtacaa
     1801 ttttgaaact aggaggataa atggaaagca ttcttaaact gcaagctgac atctgtaaaa
     1861 tattcgccaa tgacaaacgt ctggaaatga tcaatttgct aaaggacaaa gaaatgtcca
     1921 acagcgaatt aatgcaaaag actggtctga gtaaggtgac tatgactcag cacatgaatg
     1981 ttttaaagtc gaaaggcgtc attgcggtac gacgtgaagg tgttcagtta tattaccgga
     2041 tcgccaatcc taaaatcatg caggcctgca acctgatgaa agaagtttta atcgaacagc
     2101 tccaggaaaa agaaaagatg gcttccagac tggttaaagc ttcaaataaa cttaaagcac
     2161 gaatagcaag cagaaaggaa taagcgggac gcccttaagt ttttaagtta ctatcccaat
     2221 cgctgctaat caaacgtaag cgggggtggt tctatttaaa tagtgcaaga aaacaaaaaa
     2281 gacctgttac aataaactac aattaattag caaaaataat cgctaaggga ggaaaggatt
     2341 atgtctaaaa gtttaatgga atatttcaac aaacaacccc gtctcggagt gctcagcaca
     2401 agcaacaaag caggagaggt caattcggcc tatttcggat cgccgcgcat gattgacgaa
     2461 tcaacgatga ttgtggccct gggcaaaaat aaaactttag ccaaccttca ggaaaatccc
     2521 aatgcctgct atatgatcat ggagcccggt aaaagtatat ttgaatggaa aggagttcgc
     2581 atctatctga agatgaccag gtgcgagaca tcaggcgaca acgttgatat gatgaaaaaa
     2641 gcgataagtg aaaaggccgg agaagcagct gccaagatga ttcatgcggc agtaacgttt
     2701 tcggttgcca gtgccagacc gcttgccgat ttcaatcagg gttgggagaa gtctatctaa
     2761 taatcagtgg acataaatca aattccgggg acataacact taattattaa cgcatcggga
     2821 aaagcgaaat ttttatgggc atagctgata taattggatt cacggcggcg gtcatctgtt
     2881 cactcgccat gataccccag gtcgtcaaga tataccagac aaaggaaacc cgcgatctat
     2941 cgctgggcgc cttcgcggct ctgggaaccg gcctctttct ctggttagtt tatggccttt
     3001 tgatacactc ggtcccggtc attttcggca atgcggtggg tttgagtctt gttctgtatg
     3061 tcatcatcat gaagctcata catggataga aagaaataaa gagataatcg tgaacggttc
     3121 aattgaaggg acacgtccgg catacgccgg atttcatatc ttgttaaacg tgaagccgaa
     3181 gcccggcatt cagcgggcaa agcgccaggg gataacaaaa tagaaatatc aaatatcttg
     3241 caaaatttct ctaaatatat ataattaaca aaaatgttaa ttatgtgtcc ttagtagaaa
     3301 tatgaccaat ttacaggttt tgagcagaaa agattatttt accgttgagg atgtctcgca
     3361 gagtttcggc cttaagccgg catccgcgcg cgttttttgc agccgttatg tccggcaggg
     3421 actacttgtc aggttaaaaa ataattttta tacaacaacg tg
//
