LOCUS       DASVJL010000001         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_281888, whole genome shotgun sequence.
ACCESSION   DASVJL010000001 DASVJL010000000
VERSION     DASVJL010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281888"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VF357_00005"
     CDS             <1..851
                     /locus_tag="VF357_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7522554.1"
                     /translation="SDSSSPTLTERGLKYFFRVSPHDAIFARDQLQFLKDLEKAKGQK
                     IATIAALYENTEFGANVGKQILKLAPEFGYKVVADISYAANATDVTSEVGRLINAKPD
                     VLMHASYITDAILFTKTFREMGFVPKGLMTFAGYIEPGYLPAVKADGNDIIVRSTFAL
                     DLAKNKPLVGKVNALFKKKYGIDMSENAARSFTAPFVLADAMNRAKTTEAEAVVKALL
                     STNIPGDQVIYPWKGIQFDPQSHQNIHARGILVQIQNQNYVTVWPFESAAADGIWPFP
                     AWKTRK"
     gene            916..>1638
                     /locus_tag="VF357_00010"
     CDS             916..>1638
                     /locus_tag="VF357_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7522555.1"
                     /translation="MSEQLLAQLINGLLIGFMYSLIAVGLTLIWGVMNIVNFAHADFL
                     MIGMFTSFWLYTLYGVDPLLSIPVCAVLLFLLGWIIYKYVVSKVMTGPALAQVVVTFG
                     VSIFIANAAVFLWSPDFRMIEKPLLQGTWILGPVRLSIPKAVASLGSVAVSVALFLFL
                     RKTKTGKAILAVEMNRSSALLMGINTDRVNALSFAIGSSLVGIAGAFLSMYYYIYPQV
                     GGTFGLLSFCIVALGGFGSIEGA"
BASE COUNT          304 a          547 c          482 g          305 t
ORIGIN      
        1 gctccgactc ctcgtctccc accctcaccg agcgaggctt gaagtacttc ttccgggtca
       61 gcccccacga cgcgatcttc gcgcgcgacc agctccagtt cctgaaggac ctcgagaaag
      121 ccaaggggca gaagatcgct acgatcgccg ccctctacga gaacaccgag ttcggcgcca
      181 acgtcggtaa acagatcctg aaactcgcgc cggagttcgg ctacaaggtc gtcgcggaca
      241 tctcctacgc ggcgaacgcc accgacgtga ccagcgaggt cgggcggctc atcaatgcga
      301 agcccgacgt gctcatgcac gcctcctaca tcaccgacgc catcctcttc accaagacct
      361 tccgggagat ggggttcgtc ccgaaagggc tcatgacgtt cgccggctac atcgagccgg
      421 ggtacctgcc ggccgtcaag gcggacggaa acgacatcat cgtacggtcc accttcgccc
      481 tcgacctcgc gaagaacaag ccgctggtcg gaaaggtgaa cgcgctgttc aagaagaagt
      541 acggcatcga catgagcgaa aacgccgccc gcagcttcac cgcccccttc gtcctcgccg
      601 acgcgatgaa ccgcgcgaag accacggagg ccgaggcggt ggtgaaggcg ctcctttcca
      661 cgaacatccc gggcgaccag gtcatctacc cgtggaaggg gatccagttc gacccgcaga
      721 gccaccagaa catccacgcg cgggggatcc tcgtgcagat ccagaaccag aattacgtca
      781 ccgtgtggcc gtttgaatcg gccgccgcag atgggatctg gccgttcccc gcctggaaaa
      841 cgcggaagta acgtttcgcg ggggggggtc gcaggcggaa tccctccccc gccaacattc
      901 ctagaggggc caaccatgtc cgaacaactg ctggcgcagc tcataaacgg cctgctcatc
      961 ggcttcatgt actccctgat cgccgtcggc ctcaccctga tctggggcgt gatgaacatc
     1021 gtcaacttcg cccacgccga cttcctcatg atcggcatgt tcacctcctt ctggctgtac
     1081 accctgtacg gggtggatcc cctcctttcc atcccggtat gcgccgtgct cctcttcctc
     1141 ctcggatgga tcatctacaa gtatgtcgtg agcaaagtga tgacggggcc ggcgctggcg
     1201 caggtagtgg tgacgttcgg cgtctccatc ttcatcgcga acgccgcggt cttcctgtgg
     1261 tcgcccgatt tccggatgat cgagaagccg ctgctgcagg gtacatggat cctgggcccc
     1321 gttcggctct cgatcccgaa ggcggtcgcc tccctcggca gcgtcgcggt gtcggtggcg
     1381 ctcttccttt tcctgagaaa gaccaagacg gggaaggcga tcctggccgt cgagatgaac
     1441 cgcagctcgg ccctgctgat ggggatcaac accgatcggg tgaacgccct gtccttcgcc
     1501 atcggatctt ccctggtggg aatcgcggga gcgttcctgt cgatgtacta ctacatctac
     1561 ccgcaggtgg gcgggacgtt cggcctgctc tccttctgca tcgtggccct cgggggcttc
     1621 gggagcatcg agggggcg
//
LOCUS       DASVJL010000002         1966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_94371, whole genome shotgun sequence.
ACCESSION   DASVJL010000002 DASVJL010000000
VERSION     DASVJL010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94371"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VF357_00015"
     CDS             <1..540
                     /locus_tag="VF357_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522556.1"
                     /translation="SEAIVSDTSADFFRGETFVRVRSKPGVGSRNDVVGISSEIVALL
                     AGSGSPPEEARALDGLPGRVSGSILYQKRAMLGYECLAPGFEAKFSTASSSGHYLLLP
                     PAADGGVGRKGRLARELPAYREVNPTLFNAQIPSGNVWLTQEGGCVIAVAGKISRSLA
                     EPLLSSFARRLQGICRPAP"
     gene            614..1573
                     /locus_tag="VF357_00020"
     CDS             614..1573
                     /locus_tag="VF357_00020"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HEX7522557.1"
                     /translation="MKNRSFVVTAICIFAAMFLFMTVDTFARVGGSRSSGSRGSRSLS
                     TPTRPYSPATPSSTSTSPSTSTSATPTPGATPSQVPPPQTGGFMRSMMGGLAGGFLGS
                     MLFGGLGGMGGGGGLGGILPILLLVGIGYLAYRIYARRRQEQAAWSPRVEGTTPRPYA
                     TPAVVADTAGEDRNKGLEYIRRMDSAFDEKTFGETASDVFFRLQGGWTRRDLSPVSGL
                     LTDEMRATLQADADALKAKGQINRLENISVRNVEITEAWQESGQDYVTVLFQANLLDY
                     TTDEAGKVLSGSDAVPVKFEEFWTFTRPVGPGAWRLSAIQQTS"
BASE COUNT          317 a          621 c          670 g          358 t
ORIGIN      
        1 tcggaggcga tcgtctcgga cacgtcggcc gacttcttcc ggggcgaaac gttcgtgcgc
       61 gtccgttcga agcccggagt cggctcccgg aacgacgtgg tcgggatctc ctcggagatc
      121 gtcgcgcttc tcgccgggag cggttctccg ccggaggagg cgagggcgct ggacggcctg
      181 ccggggaggg tctccggatc gatcctctat cagaaacggg cgatgttggg ctacgagtgc
      241 ctggcccccg gtttcgaggc gaagttctcc accgcctcct cttccggcca ctatctgctc
      301 ctgccgcccg ctgcggacgg cggcgttggg cggaaggggc gccttgcacg ggagctcccg
      361 gcgtaccggg aggtgaatcc gaccttgttc aacgcacaga tcccttccgg caacgtgtgg
      421 ttgacccagg agggcggatg cgtcatcgcc gtggcgggga agatctcccg ttcgctggcc
      481 gagccgctcc tttcgtcctt cgcgcggcgt ttgcagggga tctgccggcc tgccccttga
      541 gtccggacac gttgacgcgg cggggcgccc cgcggtacct tgctcccgat cagcatccga
      601 tccgggaggg accatgaaga accgatcgtt cgtcgtcacg gccatctgta ttttcgccgc
      661 catgttcctg ttcatgaccg tggatacgtt cgcgcgggtc ggggggagcc gttcctccgg
      721 cagccgcgga tcgcggagct tatcgacgcc gacccggccc tactcgccgg cgaccccgtc
      781 gtccacgtcc acgtctcctt cgacgtcgac atccgcgacg ccgaccccgg gggcgacgcc
      841 gtcccaggtt cccccgcccc agacgggcgg attcatgcgc agcatgatgg ggggactggc
      901 gggcgggttc ctcgggagca tgctcttcgg cggactgggc ggcatgggcg gcggcggagg
      961 gctcggcggc atactcccca tcctccttct cgtcggcatc gggtatctgg cgtaccggat
     1021 ctatgcgagg cgccggcagg aacaggcggc ctggtcgcca cgggtcgagg ggacgactcc
     1081 ccggccgtac gcgacaccgg cggtcgtcgc cgacacggcc ggcgaggatc gcaacaaggg
     1141 gctcgagtac atccgccgga tggattccgc cttcgacgag aagacgttcg gggaaacggc
     1201 gtcggatgtc ttcttccggc tgcagggggg gtggacgcgc cgggacctgt cgccggtgtc
     1261 gggtctgctg accgacgaga tgcgcgcgac cctgcaggcg gacgcggacg cgttgaaggc
     1321 gaaggggcag atcaaccgcc tcgagaacat ctccgtgcgc aacgtcgaga tcaccgaggc
     1381 gtggcaggag tcggggcagg attacgtgac cgtgctcttc caggcgaacc tgctggacta
     1441 caccacggac gaggcgggga aggtcctctc ggggagcgat gccgtccccg tcaagttcga
     1501 ggagttctgg accttcaccc ggccggtcgg cccgggggca tggcgcctct ccgcgatcca
     1561 gcagacttcg tagcagcgga atcccccctc cgcaagccag gcggaggggg gatgaaattt
     1621 tcgcatcgtt tccccggaac gacgccggtc gtcgtttctc ccggaccggc ataggctcca
     1681 aatcggggca gctggacatt cgacgtaaat cccgtttttg acacatcttt tcttaattgt
     1741 gatccctcgg ggctcgcgaa gcccccaccg attcctattg cgagtaatta tcgaggcgtt
     1801 cgggacggaa gaagattatt attgaaaatc aattggttgc aatgtgcttc ccagtgaaag
     1861 acataaaccg cgagccggtg gaacgggaat tgctgcctga ggtacgagac gagatttgtt
     1921 tcccccgatc atcccaccgt tcgaggagga gagcgatgcg aaggaa
//
LOCUS       DASVJL010000003         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_94380, whole genome shotgun sequence.
ACCESSION   DASVJL010000003 DASVJL010000000
VERSION     DASVJL010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94380"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /gene="folE"
                     /locus_tag="VF357_00025"
     CDS             <1..399
                     /gene="folE"
                     /locus_tag="VF357_00025"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX7522558.1"
                     /translation="QYDEMVTIKDIDIFSMCEHHMLPFFGKCHVAYMPRKHIVGLSKI
                     ARVVELYARRLQVQERLTQEIATALMDTLQPYGVAVVVEAFHLCMMMRGVEKQNAKAV
                     TSAMLGVFRTRESTRMEFLELIKPNLNIVR"
     gene            complement(618..824)
                     /locus_tag="VF357_00030"
     CDS             complement(618..824)
                     /locus_tag="VF357_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7522559.1"
                     /translation="MPLYEYLCEKCGQQYESYKRLTEEKKEETCPACGGRAVKMGISL
                     STAKGTPSGAGSSCGVRPRRSPFG"
     gene            complement(836..1027)
                     /locus_tag="VF357_00035"
     CDS             complement(836..1027)
                     /locus_tag="VF357_00035"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX7522560.1"
                     /translation="MNVERSIRVIAGTFVLVSLGLGWYVSPWFLLFTVFVGLNLFQSG
                     WTNWCLMEKILIKLGVPER"
     gene            complement(1247..>2474)
                     /locus_tag="VF357_00040"
     CDS             complement(1247..>2474)
                     /locus_tag="VF357_00040"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7522561.1"
                     /translation="EVLRASFGPAQHDCPMGELGEGGKPRVDVEGTLVRADGRIVPVS
                     ASWAFFASETGEVHVFVISFRSFEEIERIAEERKSKFPFRDIVGKTPRIRRIFDLVEV
                     VKDTDSTVLITGESGTGKGLFARAIHELSPRREKPFVKVNAAALTETLLESELFGHVK
                     GAFTGAVADKVGRFEAADGGTIFLDEIGEISPALQVKLLRVLQDHEFERVGSSRTQRA
                     DVRVIAATNRELKEEMRAGRFREDLFYRLNVIPLVVPPLRERREDIPLLVDHVLKSLR
                     KRGLDRVRAVSPEAMRRLMEYPWPGNVRELENVLERGAVCSRGAVLGMEDIADEVREH
                     GRARPKETTAVPDVGLPATREDALHRPTDDAPAGERDLLLRALEEHRWNKGAAAAKLG
                     IDRSTLWRKMKRLGIA"
BASE COUNT          467 a          845 c          736 g          426 t
ORIGIN      
        1 cagtacgacg agatggtgac gatcaaggac atcgacatct tctcgatgtg cgagcaccac
       61 atgctgccgt tcttcgggaa gtgccacgtg gcgtacatgc cgcgcaagca catcgtcggg
      121 ctgtcgaaga tcgcccgcgt cgtcgagctc tacgcccgcc ggctccaggt ccaggagcgg
      181 ctgacgcagg agatcgcgac cgccctcatg gacacgctcc agccgtacgg cgtggcggtg
      241 gtcgtcgagg cgtttcacct gtgcatgatg atgcgcggcg tggagaagca gaacgccaag
      301 gcggtcacct cggcgatgct tggggtgttc cgcacccggg aatcgacgcg gatggagttc
      361 ctggaactga tcaagcccaa cttgaacatt gtgagatagc cggcgcacgg ccggcggtca
      421 gcacctgggg cctcaaccgc cccccgagcc accccgcaac agaaacacga cgaagaccgc
      481 ggcgaacagg atcaacggca tccactcacc ccacatagga acacctcaca gggaaaattt
      541 gcctccatcg ccatgcctgg agggcgatcc cgcgcaagat aaggcgtacc cggcgaccgc
      601 cgggcaatgg atcacgctca tccgaacggc gagcggcgcg ggcggacacc gcaggaggag
      661 ccggccccgg acggggttcc cttcgcggtg gacagggaga tccccatttt caccgcacgg
      721 ccgccgcacg cggggcacgt ctcttccttc ttctcctcgg tcagccgctt gtacgactcg
      781 tactgctgac cgcacttttc acaaagatac tcgtagagag gcatcggtat tccccctatc
      841 tctcgggcac gcctaatttg atcaggatct tttccatcag gcaccagttc gtccaccccg
      901 actggaacag gttgagcccc acgaacacgg tgaacagcag gaaccaggga gaaacgtacc
      961 accccagccc aaggctcacc agcacgaagg tccccgcgat cacccggatc gaccgttcga
     1021 cgttcatcgc atcctcccca tttccacccc cggttggatg tcttcgggag tcttccgttg
     1081 cggaaggtaa atgcatttgt agtgccaacg ataggaaacg aacgttagat acttaagata
     1141 gcgaacggta atacaaataa ggtaataatc gaaaagcatg atggcgcatg ctccttgggg
     1201 acgcacattc cgtttcataa aatgcaacat tttgtatcag cacgggtcag gcaatgccga
     1261 gccgtttcat ctttcgccaa agggtggagc gatcgatccc cagctttgct gcggccgctc
     1321 ccttgttcca ccggtgctcc tcgagcgccc gaagcaggag gtccctctcc cccgccggcg
     1381 catcgtccgt cggccgatgg agagcgtctt cccgggtcgc agggaggccg acgtcgggaa
     1441 cggcagttgt ctccttcggc cgagcgcggc catgctcccg cacctcgtcc gcgatatcct
     1501 ccatcccgag caccgcgccc cgggagcaga cggccccccg ctccagcacg ttctcgagct
     1561 cccgcacgtt gccgggccac ggatactcca tcaggcggcg catcgcctcg ggggagacgg
     1621 cgcgcacccg atcgagcccg cgcttccgga gagacttcag gacgtgatcg accagcaggg
     1681 ggatgtcctc ccggcgctcg cgtagcggcg gaacgacgag ggggatgacg ttcaggcggt
     1741 agaagagatc ctcccggaac cgcccggcac gcatctcttc cttcagctcc cggttcgtgg
     1801 cggcgatcac gcggacgtcc gcccgctggg tcctgctgct cccgacgcgc tcgaactcgt
     1861 ggtcctggag gacgcgcagc agcttcacct ggagggccgg ggagatctcg ccgatctcgt
     1921 cgaggaagat cgtcccgccg tcggccgcct cgaaccgccc caccttgtcc gccacggctc
     1981 cggtgaaggc ccccttcacg tgaccgaaca gctcggactc gagcagcgtt tccgtgaggg
     2041 ccgccgcgtt gaccttgacg aacggcttct ctctccgggg ggacagctcg tggatcgctc
     2101 gggcgaacag ccctttcccg gttccgctct ccccggtgat cagcaccgtg gaatccgtat
     2161 ccttcaccac ctcgacgagg tcgaagatcc ttcggatccg cggcgtcttt ccgacgatgt
     2221 cgcggaacgg gaatttcgac ttccgctcct cggcgatcct ctcgatctcc tcgaagctgc
     2281 ggaaggagat gacgaagacg tgcacctcgc ccgtctcgct cgcaaagaac gcccaactcg
     2341 cggagacggg aacgatgcgc ccgtccgcgc ggacgagggt cccttcgacg tcgacccggg
     2401 gttttccccc ctcgccaagc tcccccatcg ggcaatcgtg ctgcgcgggg ccgaacgagg
     2461 cgcgaaggac ctcc
//
LOCUS       DASVJL010000004         4043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_375791, whole genome shotgun sequence.
ACCESSION   DASVJL010000004 DASVJL010000000
VERSION     DASVJL010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375791"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VF357_00045"
     CDS             complement(<1..305)
                     /locus_tag="VF357_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7522562.1"
                     /translation="MPLSQKELTRALFGRVASKYRCSADHTDMEDLDLLFTGLALDPG
                     HRILDVATGGGHTAAALAGRSGRVVASDLTPSMLREARGLAAEHRAGNVLFTAADAEA
                     "
     gene            381..3596
                     /locus_tag="VF357_00050"
     CDS             381..3596
                     /locus_tag="VF357_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX7522563.1"
                     /translation="MPDRLPAISRLYAGPFPALEERLIGRYFGRAGRDPSLEHVLLVP
                     SNELREHLLKRLASGPGVASVFAGASVMTLYDFAVRLLKHRGIFPAELPPARMAAAVL
                     AAVRDTYASGGGDFEGISATPGFIPALARTLSDLEEGWVGDAELSLTERAAKDRGDAR
                     KAARWAEWRRLRAAVGRKVRAMGGETRRRIFREAVAGFEQPGYPFRATLYGFYDFTRL
                     QWMLVERLLSSGLLDEVYFPGLFDGEGNLRPSFLYAARAWDRLRAAFEGNVEFLADVP
                     SAGIALVRERIFSPTPPAERTPAPFAVLSAPHAEGEMRLAARRVRSWRDAEPGASVVL
                     VARKVTPEMAADWERIAAEYGIGTAGRLDVPLSSVPLVRLLLRMIAVARDDFPRREVI
                     DVLSSPYRRFAGGEGEIAARPDLWDLLSKELLVVSGSDWETRLARPPKRRRAGEEEET
                     EADGRTAQVALLRSEVRALRASLRSFAEARGYAAFAQAVRALLVREFHVVRDDAPEWE
                     RDRRAVEALFAILDDLEAIPDREAPWPGAREGAEGFSALLSAQRLFVGERGGMRKPGA
                     VVLGDSVVLRGVTADRAIVLSVNEDAVPAQLEEDPLLPDDDREEINRGTRQPDLPDAL
                     SLRRRNAAEEKLLFALPAASVREEIAFSVLRADAAGAARRPSRYLLHLLSRFAGPAVF
                     SEEWEAASGAVVERLPRSPFLALEGEGPRSARETALRTWRAGAEPEVVAAGVPWGKVR
                     GILAAWGARSAGEGIFPGPGLRVRLPPWHSASALEELARCPYRYFLRRVVRLDPPEEP
                     EEALAISPAELGEIAHDILRRLGRDAAAGKGWGNVSAAARSEVDRFARENPTGLPGLF
                     RIRCRGVERDVARLAAWERKQETECPGWRVDRVEEAFSLPAAPLLPALRGRVDRIDRG
                     PAGEARVIDYKYRDPARGEIFPDWIRHGLSHQVPVYLAFVASLLPAPSAVSAAFYFLR
                     NEFGVEEAPGWDEIRGEWAAALADWLSLAAAGAFPPLPHHRFTSAGRTSPRYCDSCPF
                     RDHCRVSPVYDGSEIGPGALAARIAREPALRPVADHRPGKE"
     gene            3600..>4043
                     /locus_tag="VF357_00055"
     CDS             3600..>4043
                     /locus_tag="VF357_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7522564.1"
                     /translation="MRENREKAANEFRRDLAVDASAGTGKTSTLVARATNLFLARPDL
                     STDHVLLLTFTDKAAAEMKARVTEGWERLFAATQETDSIDEVGRRAAAWNPLARVPAT
                     VYPDFATLRRRVEEMVDGVGRLSVTTFHSFCARVLRSFPAEAGVDP"
BASE COUNT          489 a         1433 c         1494 g          627 t
ORIGIN      
        1 gcctcggcgt cggcggccgt gaagaggacg ttcccggccc ggtgctctgc ggccagaccc
       61 cgcgcctccc gcagcatgga aggggtgagg tccgacgcca ccacccgtcc ggagcgcccg
      121 gcgagtgccg ccgcggtgtg cccgccgccg gtggccacgt cgaggatccg gtgccccggg
      181 tcgagggcga ggcctgtgaa gaggagatcg agatcctcca tgtccgtgtg gtcggcgctg
      241 cagcggtatt tcgacgccac ccggccgaac agcgcacggg tgagctcctt ctgggacaac
      301 ggcatccgcg cttcctccgc cccctatttt caccctcagg ctcgacgatg acaaggccgt
      361 atgcggggat acaatggggt atgccggacc ggctccccgc catctcgcga ctgtacgccg
      421 gaccgtttcc cgcgctcgag gaacggctga tcgggcgata tttcggccgc gccgggcggg
      481 acccttcgct ggagcacgtt ctcctcgtac cgtccaacga actccgggag cacctcctga
      541 agcgcctggc gtccggtccc ggcgtcgcct ccgtcttcgc cggcgcttcc gtgatgacgc
      601 tgtacgactt cgccgtgcgc ctgctgaagc atcgggggat cttcccggcg gaactcccgc
      661 cggcgcggat ggcggcggcg gtcctcgcgg cggtgcggga tacgtacgct tcggggggcg
      721 gggatttcga ggggatctcc gcgacgcccg gcttcatccc cgccctcgcc cgtacgcttt
      781 ccgacctcga ggagggatgg gtgggggacg cggagctctc gttgacggaa agggcggcga
      841 aggaccgggg agacgcccga aaggcggcgc ggtgggcgga gtggcggaga ctgcgggcgg
      901 ccgtgggccg gaaggtccgg gcgatggggg gagagacgcg acgccggatc ttccgggagg
      961 cggtggcggg gttcgagcag cccggctacc cgttccgagc gaccctgtac ggcttctacg
     1021 atttcacccg gcttcagtgg atgctggtcg agcggctcct ttcttcgggc ctgctggacg
     1081 aggtctattt tcccggtctc ttcgacggcg aggggaacct ccgtccttcg ttcctgtacg
     1141 ccgccagggc gtgggatcgt ctgcgcgccg ccttcgaggg aaacgtggag tttctggccg
     1201 acgtcccttc cgccgggatc gcgttggtgc gcgagcggat cttctccccc accccgcccg
     1261 cggaaaggac gcccgcgccg ttcgccgtcc tctccgctcc ccacgcggaa ggggagatgc
     1321 ggctcgccgc ccgccgggtc cggtcctggc gggacgccga acccggcgcc tccgtcgtcc
     1381 tcgtcgcgag gaaggtgacc ccggaaatgg cggccgattg ggagcggatc gccgcggagt
     1441 acggcatcgg cacggcgggg cgcctcgacg tccccctctc ttccgttcct ctcgtgcgcc
     1501 tccttctgcg gatgatcgcg gtcgcacggg acgacttccc gcgccgggag gtgatcgacg
     1561 tcctctcgtc cccgtatcgt cgtttcgcag ggggggaggg cgagatcgcc gcgcggcccg
     1621 acctgtggga cctcctttcg aaggagctct tggtcgtttc cgggtccgat tgggaaacgc
     1681 gactggcccg gcctccgaag aggcgccgtg cgggggagga agaggaaacg gaggcggacg
     1741 gccgcacggc gcaggtcgcc ctgctccggt ccgaggtgcg cgccttgaga gcctccctgc
     1801 ggtccttcgc ggaggcgcgt gggtacgccg ccttcgccca ggcggtgcgg gcccttctcg
     1861 ttcgagagtt ccacgtcgtt cgggacgatg cccccgagtg ggaacgcgat cgccgggcgg
     1921 tggaggcgct cttcgcgatc ctcgacgacc tggaggcgat ccccgaccgc gaggcgccgt
     1981 ggccgggggc gcgggagggg gcggagggat tttcggccct cctctccgcg cagcggctct
     2041 tcgtcggaga gcgcggagga atgcgcaagc ccggcgcggt cgtcctcggg gactccgtcg
     2101 tcctgcgggg cgtcacggcg gaccgggcga tcgtcctgtc ggtgaacgag gacgcggtgc
     2161 cggcccagct cgaggaggac ccgctcctcc cggacgacga tcgggaggag atcaaccgcg
     2221 ggacgcggca gccggatttg ccggacgcct tgtcgctgcg gcggcgcaac gcagcggagg
     2281 agaagctcct cttcgccctt cccgccgcct ccgtgcggga ggagatcgcc ttctcggtcc
     2341 tgcgcgccga cgcggcgggc gcggcccggc gcccctcccg gtacctgctc cacctgttgt
     2401 cgcgcttcgc cggccccgcg gttttctccg aggagtggga ggccgcctcc ggtgccgtcg
     2461 tcgaacgcct cccccggtct ccgttcctcg ccctcgaggg cgaggggccg cggagcgccc
     2521 gggagacggc gctgcgcaca tggcgcgcgg gagcggagcc ggaggtcgtc gccgccggcg
     2581 tgccgtgggg caaggtgcgc gggatcctcg ccgcgtgggg agcgcggtcc gcgggggagg
     2641 ggatcttccc cggcccgggt ctccgcgtcc gcctcccgcc ctggcacagc gcctccgcgc
     2701 tggaggagct ggcccgctgc ccgtaccggt acttcctgcg gcgcgtcgtc cgcctcgatc
     2761 cgccggagga gcccgaggaa gcgctcgcga tctcccccgc ggaactgggg gagatcgccc
     2821 acgacatatt gcgccgcctc ggtcgggacg cggcggcggg aaaggggtgg gggaacgtct
     2881 cggccgccgc gcgaagcgaa gtggatcgct tcgcccggga gaacccgacc ggcctccccg
     2941 gcctcttccg gatccgctgc cggggcgtgg agcgggacgt tgcgcgcctg gcggcgtggg
     3001 agcggaaaca ggagaccgag tgccccgggt ggcgggtcga ccgggtcgaa gaggcgttct
     3061 cccttcccgc cgccccactc cttccggcgc ttcgcgggcg ggtcgaccgg atcgaccggg
     3121 gccccgcggg ggaagcccgg gtgatcgatt acaagtatcg cgacccggcc cgcggggaga
     3181 tcttcccgga ctggatccgt cacggcctct cccaccaggt ccccgtctat ctggcgttcg
     3241 tggcgtcgct cctccccgcg ccgtccgccg tctcggcggc cttctacttt ctgcgcaacg
     3301 agttcggggt cgaggaggcg ccggggtggg acgagatccg aggggagtgg gccgccgccc
     3361 tcgccgactg gctctccctc gccgccgcgg gtgcgttccc tccgctgccg caccaccggt
     3421 tcaccagcgc cggccggacg tcgccccgat attgcgattc ctgcccgttc cgggatcatt
     3481 gccgcgtctc gcccgtctac gacggctcgg agatcggccc cggcgcgctc gccgcgcgga
     3541 tcgcgcggga accggcgctg cgtccggtgg ccgaccaccg gcccgggaag gagtaggcgg
     3601 tgcgggaaaa cagggaaaaa gcggccaacg agttccggcg cgacctcgcc gtcgacgcat
     3661 ccgcggggac ggggaaaacg tccaccctcg tggcgcgggc caccaacctt ttccttgccc
     3721 gcccggacct ttccaccgac cacgtcctcc tcctgacctt taccgacaag gcggcggcgg
     3781 aaatgaaggc gcgggtcacg gaggggtggg agcggctctt cgcggcgacg caggagaccg
     3841 actccatcga cgaggtcggg cggcgcgcgg cggcgtggaa tccgctcgcc cgcgttcccg
     3901 cgacggtcta tcccgacttc gccacgctgc gtcggcgggt cgaggagatg gtcgacggcg
     3961 tgggccgcct ctccgtgacg acgttccact cgttctgcgc ccgcgttctc cgctccttcc
     4021 cggcggaggc gggggtcgac ccc
//
LOCUS       DASVJL010000005         5038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1501552, whole genome shotgun sequence.
ACCESSION   DASVJL010000005 DASVJL010000000
VERSION     DASVJL010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501552"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VF357_00060"
     CDS             <1..1165
                     /locus_tag="VF357_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7522565.1"
                     /translation="NFRGPHRTFPHLSAAAVLEGTEDPARLKGRIVLLGTTAAGLKEI
                     RTTPLDAALSGVEIQANVLDNLLSGDPIAEPRWARAVRILLVLFPGFLLTGLLARSSA
                     AWGLALIVPSAAAIWLGSWGLLAYRQVFLPPLLPVVTLTIVFTVLASMRFLRADRQVR
                     ERTRKLALTQDAIIQSLAALTETRHHETGSHIQRTRHYIRVLAGRLQAHPGFRNRLDD
                     ATVDLLFRLAPLHDIGKVGVRDLILLKPDRLTPEEYEEMKRHTLYGSETIRLAKRMLG
                     EDAFFQIADDLVLNHHERWDGTGYPNGLSEEAIPLPGRLMAVADAYDAIISPRVYKPA
                     LTHEEAVRGMSEKRGTYFDPDVVDAFLEVNEEFREIAARFAGAGVEPELPPGV"
     gene            complement(1272..2564)
                     /locus_tag="VF357_00065"
     CDS             complement(1272..2564)
                     /locus_tag="VF357_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX7522566.1"
                     /translation="MLKRFPPNYRTDREMRDLQLEGLRWTVGHAWNNSPFYRKRLDES
                     GVSPDSIRSLEDLGRLPFTTADDLRDGYPLPLLAVPTRDILRIHSSSGTTGKRKILCY
                     TRKDIDDWKDMFGRCYEMAGLSREDRVQICVGYGLWTAGAGFQLGAERFGAMAIPSGP
                     GNLELQCNFLIDLQTTVLCCTASMGLLVAEEVHARGLRGRLRLRKVILGAERTSDAML
                     QTIRDLLGVEEVYDIPGLTELYGPGTGLSCRHNVGIHYWSDYYILEILDPETLVPVAP
                     GEIGEMVYTTLRKEAAPLLRYRSRDLTRMIEGPCPCGCILPRHDRILGRSDDMVVFRG
                     VNVYPGQVDEVLSRIGGIGSEYQVLLDRKADGKDYMTVKVERAADAAETDTASVARIV
                     ASEIKRNLMVSCAVDMIPYGTLPRSERKTKRVFDNRPV"
     gene            complement(2580..3128)
                     /locus_tag="VF357_00070"
     CDS             complement(2580..3128)
                     /locus_tag="VF357_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX7522567.1"
                     /translation="MRQQVVLSGVGGQGILFLSRLLAEAAIAGGFPVITSETHGMAQR
                     GGVVVSHLKVGGFDSPLVRTGRADLLLVLKEENVALHREFLADGGTLIINAPVPTEAG
                     PRVRAHAVDADALAISAGAPHAVNLILLGFALARIGGGTAGGFFCEAGEIREALSRRH
                     GGSGPRLAASLSALDLGIAHGT"
     gene            complement(3155..4984)
                     /locus_tag="VF357_00075"
     CDS             complement(3155..4984)
                     /locus_tag="VF357_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7522568.1"
                     /translation="MSDRILMQGNDAIARGLVEYGCAVAASYPGTPASEILSSIDFWR
                     KKCGAAMHVEWAVNEKVAFEIAYTAAIAGLRAAVSMKQVGLNVASDPLFSAAYLGVTA
                     GFLVVSADDPGPHSSQTEQDSRLLAMMAKVPVLDPASPAQAMALVATGFDLSEAFGIP
                     VMLRPTTRVCHACQDVDAPPFRPPAREARFVKDPARWAATPVFRRKLHDRLAEKIAAI
                     AAWPATAPIRVNPEVSPPNAVVASGVAFAHARELFGELGISGRIALYQVIQPYPLHAA
                     FIDHLLDTYAEILVLEESAPVIEMQVADRRRVRGRMTGAVPGTGELLPEIIERLLSRF
                     AGLPAPGGALPAQGGGRRPTLCPGCSHRSSFYAIRKAAPDGIYPSDIGCYTLGINLGA
                     VDTVLCMGAAVSQAAGFDYAHRLSGKQVDIVATIGDSTFYHSGIPPLIDAVVQGARFV
                     LVILDNGTTAMTGNQPTPETGIGAGGETTAAVDMEGLVRACGVRFCRTANPDRLPEFV
                     ALLKEAIVHARERGVAVVIARQPCAMVTRIPGGKEPGAIVITGECTGCRHCVDRFECP
                     ALVFDEAANRVRIDEMICTDCGVCVHVCPVGAIRQESAGEKRP"
BASE COUNT          828 a         1768 c         1657 g          785 t
ORIGIN      
        1 gaactttcgc ggaccccacc ggacctttcc ccacctttcc gccgccgcgg tcctcgaggg
       61 gaccgaggac ccggcgcggt tgaagggaag gatcgtcctg ctcgggacca ccgccgccgg
      121 gctcaaggag atccgcacca cgccgctcga cgccgccctg tccggagtgg aaatccaggc
      181 caacgtcctc gacaacctgc tttcgggaga cccgatcgcg gagccgcgat gggcgcgggc
      241 ggtgaggatc ctcctcgtcc tcttcccggg gttcctgctg acaggcctcc tggcgcggtc
      301 cagcgccgcg tgggggctcg cgctgatcgt gccgtccgcg gctgcgatct ggctcgggtc
      361 ctggggactc ctcgcgtacc ggcaggtctt ccttcccccg ctcctgccgg tggtcacgtt
      421 gacgatcgtc ttcacggtcc tcgcctccat gcggttcctg cgggccgacc ggcaggtccg
      481 cgaacgaacc cgcaaactcg cgctcacaca ggacgccatc atccagagcc tggccgccct
      541 gacggagacc cggcaccatg aaaccgggag ccacatccag cgcacccggc actacatccg
      601 ggtccttgcc ggacgcctgc aggcgcatcc ggggttccgg aaccggctgg acgacgccac
      661 cgtggatctc ctcttccgcc tcgctcccct ccacgacatc ggcaaggtcg gcgtgcgcga
      721 cctgatcctc ctgaagccgg accggttgac gccggaagag tacgaggaga tgaagcggca
      781 cacgttgtac gggtcagaga cgatccggct ggcgaaacgg atgctggggg aagacgcctt
      841 tttccagatc gcggacgatc tcgtcctcaa ccatcacgag cggtgggacg gaacggggta
      901 tccgaacggc ctgagcgagg aagcgatccc gcttccgggg cggctgatgg cggtggccga
      961 cgcctacgac gcgatcatct ccccccgcgt ctacaagccg gcgctgaccc acgaggaggc
     1021 ggtccgcggc atgagcgaaa aacgggggac gtatttcgac cccgacgtgg tggacgcctt
     1081 cctggaagtc aacgaggagt tccgcgagat cgccgcccgc ttcgccggcg ccggcgtgga
     1141 gcccgaactg cccccgggag tctgatccgc tccagcttcg ttgtgccttc atgccgagcc
     1201 ccgggggtac cccagggagt gcaattcgta cgggagtggg gcgccctccc ctgcgttcgt
     1261 cgtactccgg ttcagacggg gcggttgtcg aagacccgtt tcgtcttgcg ctcggagcgc
     1321 gggagggtcc cgtacgggat catgtcgacc gcgcaggaaa ccatcaggtt ccgcttgatc
     1381 tccgacgcga caatgcgggc gaccgacgcg gtgtcggtct cggcagcgtc cgcggcacgc
     1441 tcgaccttga ccgtcatgta atccttcccg tcggccttcc gatcgaggag aacctggtac
     1501 tcgctcccga tccccccgat ccgcgagagg acctcgtcga cctggccggg atatacgttc
     1561 acgccgcgga agacgaccat gtcgtcggaa cgcccgagga tccggtcgtg gcgcgggaga
     1621 atgcatccgc agggacacgg tccctcgatc atgcgggtca ggtcgcgcga gcggtaccgt
     1681 aggagaggcg ccgcctcctt gcgcagcgtc gtgtagacca tctcgccgat ctccccgggc
     1741 gcgacgggga cgagcgtctc cgggtcgagg atctccagga tgtagtagtc ggaccagtag
     1801 tggatcccga cattatggcg gcaggaaagg ccggtcccgg ggccatacag ttccgtcagc
     1861 ccggggatgt cgtacacctc ctcgacgccc agcaggtccc ggatcgtctg gagcatcgca
     1921 tcgctcgtcc gctcggcgcc caggatcact ttcctcaacc ggaggcgtcc ccgcaggccg
     1981 cgcgcgtgga cctcctccgc cacgaggagc cccatcgacg cggtgcagca gagcacggtc
     2041 gtctggaggt cgatcaggaa attgcactgc agctcgagat tcccgggccc ggaagggatc
     2101 gccatcgccc cgaaccgctc cgcccccagc tggaatccgg cccccgccgt ccacagcccg
     2161 tagccgacgc agatctgcac gcggtcctcg cgcgaaagcc ccgccatctc gtagcaccgg
     2221 ccgaacatgt ccttccagtc atcgatgtct ttccgcgtgt agcagaggat cttccgcttc
     2281 cccgtggtcc ccgaggagga atggatccgg aggatgtccc gcgtcgggac cgccaggagc
     2341 ggaagcgggt atccgtcccg caaatcgtcc gcggtcgtga aaggaagcct cccgaggtct
     2401 tcgagcgacc ggatggaatc gggcgatacc ccggattcgt cgaggcgctt ccggtagaag
     2461 ggggagttgt tccacgcatg tccgacggtc cagcggagac cctccagttg gaggtctcgc
     2521 atctcccggt ccgttcggta gttcggtggg aaccgtttca gcatagggag cacttcatat
     2581 cacgtcccat gggcgattcc aagatcgagg gcggaaagcg aagcggcgag ccggggcccg
     2641 ctccccccat gcctccgcga aagcgcctcc cggatctccc ctgcctcgca aaagaagccg
     2701 cccgcggtcc cgccaccgat ccgcgcgaga gcgaatccga gcaggatcag gttgaccgca
     2761 tgcggggctc cggccgaaat ggcgagcgcg tcggcatcga ccgcgtgcgc ccgcacgcgg
     2821 ggaccggctt cggtcgggac cggcgcgttg atgatcaggg tgccgccgtc ggccaggaac
     2881 tcccgatgga gggcgacgtt ctcctccttg aggacgagga gcaggtcggc ccggcccgtc
     2941 cgaaccagtg ggctgtcgaa tccgcccacc ttcaggtggg agacgaccac ccccccccgc
     3001 tgggccatcc cgtgcgtttc ggaggtgatc accgggaatc cgccggcgat cgccgcctcg
     3061 gccaggagac gggagaggaa caggatcccc tgccctccga cgccggacag gacgacttgt
     3121 tggcgcatga gagtggacct ccggacatcc gcgctcatgg acgcttctcc ccggcggact
     3181 cttgccggat cgcgcccacc gggcagacgt gaacgcacac tccgcagtcg gtgcagatca
     3241 tctcgtcgat ccggacccgg ttcgccgcct cgtcgaaaac cagggccggg cattcgaacc
     3301 ggtccacgca atgccggcac ccggtgcact ccccggtgat cacgattgcg cccggctcct
     3361 ttccgccggg gatgcgggtg accatcgcgc acggctgccg ggcgatcacc accgccacgc
     3421 cacgctcgcg ggcatgcacg atcgcctcct tgagcagcgc gacgaattcc gggagacggt
     3481 cggggttcgc cgtccggcag aatcggacgc cgcacgcgcg gacgagcccc tccatgtcga
     3541 ccgccgcggt ggtctcccct cccgcgccga tcccggtttc cggcgtcggc tggttccccg
     3601 tcatcgcggt cgtcccgttg tcgaggatca cgaggacgaa tcgcgccccc tggacgacgg
     3661 cgtcgatcaa gggtgggatc ccggagtggt aaaaggttga atcgccgatt gttgcaacga
     3721 tgtcaacctg tttcccggag agccggtgtg cgtaatcgaa gccggccgcc tggctgaccg
     3781 ccgcccccat gcacaggacc gtgtcgacgg cgccgaggtt gatgcccagt gtgtagcagc
     3841 cgatatcgct cgggtagatg ccgtcgggag ccgccttccg gatggcgtag aagctcgacc
     3901 ggtgcgaaca gcccgggcac aacgtcggcc gtcgtccgcc tccctgcgcg ggaagcgctc
     3961 cgcccggagc aggaagccct gcgaaccggg agaggagccg ctcgatgatc tcggggagca
     4021 gttcccccgt cccgggaacc gccccggtca tccgcccgcg cacgcgacgc cggtccgcaa
     4081 cctgcatctc gatcaccggc gccgactcct cgagcacgag gatttccgcg tacgtatcca
     4141 ggaggtggtc gatgaaggcg gcgtgcagcg ggtacggctg gatcacctgg tacagcgcga
     4201 tcctccccga aatccccagc tcgccgaaca gttcccttgc gtgcgcgaag gcgacgcccg
     4261 aagccaccac ggcattcggc ggcgaaacct ccggattcac ccggatcggg gcggtcgcgg
     4321 gccacgcggc gatcgccgcg atcttttcgg caaggcgatc gtgcagcttc ctgcggaaaa
     4381 cgggggtcgc cgcccaacgg gccggatctt tcacgaaccg ggcctcccgc gccggcggcc
     4441 ggaacggagg cgcgtcgacg tcctggcacg cgtggcagac gcgcgtcgtc ggtcgcagca
     4501 tcacggggat gccgaacgcc tccgagaggt cgaacccggt cgccacgagc gccatcgcct
     4561 gcgcgggaga cgccgggtcg aggaccggca ccttcgccat catcgccagc agccggctgt
     4621 cctgctccgt ctgagaggag tggggacccg ggtcgtccgc gctcacgacc aggaacccgg
     4681 ccgtcactcc gaggtaggcg gcgctgaaca acgggtcgga ggcgacattc aaccccacct
     4741 gcttcatgct gaccgcggcg cgcaacccgg cgatggcggc cgtgtaggcg atctcgaacg
     4801 cgaccttctc gttcaccgcc cattcgacgt gcatcgcagc gccgcatttt ttccgccaga
     4861 aatcgatcga ggagaggatt tcggaggcgg gcgtccccgg ataggaagcg gcgaccgcgc
     4921 agccgtactc gaccaacccg cgcgcgatgg cgtcgttccc ctgcatcagg atgcggtcgc
     4981 tcacgaggaa aactcccgcc ggtctatttc acgaggtccg acaggacgag gacggtcc
//
LOCUS       DASVJL010000006         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1501600, whole genome shotgun sequence.
ACCESSION   DASVJL010000006 DASVJL010000000
VERSION     DASVJL010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501600"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1694)
                     /gene="recN"
                     /locus_tag="VF357_00080"
     CDS             complement(15..1694)
                     /gene="recN"
                     /locus_tag="VF357_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX7522569.1"
                     /translation="MLIELTVRNLAIFEDVRVPFSAGLNVVTGETGAGKSLLVEAIRL
                     ALGEKADPVAVRTGEPEAEVTALFDLSSRDDLRDAWEEAGLPWEEEVVLRRVLPAAGR
                     SRAYLNGRPVAQPVLGALSPLLLTMVGQHSVPELLSRAAALSAVDDFAGAGALASEMR
                     KRYRRVAALRRQVAEAADRGAGARSRTETLDFEIGELSKAALAPGEEEELAADLSLLR
                     NAAKVREALQAADDAIASSENAALVSMAFALARLREAAAVDPRIVETAERLRSAREEV
                     QDLSRELGSLAAGVTEDPERRERVEERLSAIRRLKRKYGKEVPELVSHLDSLRSERES
                     LAGALEEEARLREDLVIEEEGGVAAAKKLSATRRKAAAAMGPAVEKELARVALAGARF
                     RAEVSSREAVPAALSASGIDEAELLFSANPGQELRPLAQTASGGELSRVMLCLRNAAS
                     RGGAGKTLVFDEIDTGIGGQVAERVGARLKSLSVAAQVVCVTHLPQVAAFADHHLQVV
                     KKAAAGTVATGVKPLSKQDRIGELARMISGAEITEKAKAHAKTLIEKAAGE"
BASE COUNT          235 a          684 c          588 g          257 t
ORIGIN      
        1 cctcgctttc cggatcattc ccccgccgcc ttctcgatca gcgtcttcgc gtgcgccttc
       61 gctttttccg tgatctccgc gccggagatc atccgggcga gttcccctat cctatcctgt
      121 ttcgacagcg gtttcacacc ggtggcgacc gttccggccg ccgccttttt cacgacctgg
      181 aggtggtggt cggcgaaggc cgcgacctgt ggcaggtgcg tgacgcagac gacctgcgcg
      241 gcgacggaaa ggcttttcaa ccgcgcaccg acgcgttcgg ccacctggcc cccgatcccc
      301 gtgtcgatct cgtcgaacac gagcgtcttt ccggcgcccc cccgggaggc ggcgttccgc
      361 aggcaaagca tcacgcggga gagctccccg cccgaggcgg tctgcgcgag cggtcgaagc
      421 tcctggccgg ggttcgcgga gaagaggagc tccgcctcgt cgatccccga cgccgaaagc
      481 gccgcgggga ccgcctcgcg ggaggaaacc tccgcccgga aacgcgcccc ggcgagggcg
      541 acccgcgcga gttccttttc caccgccggt cccatcgccg ccgccgcctt ccggcgcgtc
      601 gcggagagtt tcttcgccgc cgcgaccccg ccctcctcct cgatgacgag atcttctcgc
      661 agccgggcct cctcttcgag cgccccggcc agcgactcgc gctccgaccg gagggaatcg
      721 aggtgggata cgagctcggg aacctccttg ccgtacttcc gcttgaggcg gcggatcgcg
      781 ctcagccgct cctcgacgcg ctcgcggcgc tcgggatcct ccgtcacccc ggccgcgagg
      841 gatccgagct cccgtgagag atcctgcacc tcctcgcggg cggagcggag ccgctcggcg
      901 gtttctacga tccgcgggtc gaccgcggcc gcctcccgca ggcgcgcgag ggcgaacgcc
      961 atcgatacga gcgcggcgtt ctccgacgac gcgatcgcgt cgtccgccgc ctggagcgcc
     1021 tcgcgcacct tcgccgcgtt gcgcagcagc gaaagatccg ccgcgagctc ctcctcctcg
     1081 ccgggggcga gcgccgcctt cgacagctcc ccgatctcga agtcgagggt ctcggtccgg
     1141 ctccgggcgc cggccccgcg gtccgccgcc tccgcgacct gccggcgcag cgccgccacg
     1201 cggcgatacc gtttccgcat ctccgaggcg agcgcccccg cgccggcgaa gtcgtcgacg
     1261 gccgacagcg ccgcggcgcg ggacagaagc tccggcacgc tgtgctgccc gaccatcgtg
     1321 aggagaagcg gggagagcgc cccaagcacg ggctgggcca cgggacgccc gttcaggtag
     1381 gcccggctcc gcccggcggc cgggaggacg cgccgcagca cgacctcctc ctcccacggc
     1441 agccccgcct cctcccacgc gtcgcgcagg tcgtcgcggc tggagagatc gaacagggcg
     1501 gtcacctccg cctcgggctc cccggtgcgc accgctacgg gatcggcctt ctcgccgagg
     1561 gcgaggcgga tcgcctcgac caggagggac ttccccgcgc ccgtctcccc cgtgaccacg
     1621 ttcagacccg cggagaacgg gacacggacg tcctcgaaga tggccaggtt gcggacggtg
     1681 agctcgatca gcatcgcggg gggacggtca ccgctctccc cacttcagct tggtgcgcag
     1741 gacggtgaag taatcccgga aggg
//
LOCUS       DASVJL010000007         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_282126, whole genome shotgun sequence.
ACCESSION   DASVJL010000007 DASVJL010000000
VERSION     DASVJL010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282126"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VF357_00085"
     CDS             complement(<1..660)
                     /locus_tag="VF357_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7522570.1"
                     /translation="MRKLLIGVVLAAALAGAAYLYLKKDNGTPNYRTAKVERGDIVDT
                     ITATGNINAVTTVSVGSQISGTIRKLFVDYNSRVRKNEVIAQIDPQLLEASLIQAKGS
                     LENAKGSLEKAKVSVTDTERTYRRNRELLPNGFVAQSDVDAAQTAYDQAVAAQKSAEA
                     GVTQALGALKVAQTNLEYATIRSPVEGTVISRNVDVGQTVAASFQTPTLFSIAQDLTK
                     MQ"
     gene            complement(657..2153)
                     /locus_tag="VF357_00090"
     CDS             complement(657..2153)
                     /locus_tag="VF357_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7522571.1"
                     /translation="MTPLLRALPLLLVLLCAYPFSAAGAETLSLSIGDAVRIAVEKNL
                     GLQVATYSPAIAETGIRKARAIYDPLLSALVDYQGSNRQAAPQSFFVDRQRDFQFDAS
                     ISQLLPSGATTSLSFNNFWSRDNLGLPTSRFAQPQLTLSFSQPLLQGLGREVTERGIT
                     TADDATDSALADWTQQALNTAALARNQYLVLMKTRESLATRQTSLALAREVQAQNEGR
                     VDAGVLAAYQLQDSRLGVLQAQKNLLDAKRAEMDAADQLLTILHLEAGTAVATTPPPP
                     ETSEVSEPDAVRTAMQRRPDIVKARVAVRTAEFNEKVSRNLALPSLALQGSSGITGLD
                     SSYGDAFGDMGSARYPIWSVGLSFSVPIGNNSARADVAASRLIASQARTQLAATEESA
                     TLEVRTALRALTSAQDQLGVTEQGVTAAEVLMDSYLKRQQLGLATTKDVLDVSASLTQ
                     ARENYTAARADYQSAFTNLWKATGELLDRQGILLKGNISASRERKGTP"
     gene            2298..2888
                     /locus_tag="VF357_00095"
     CDS             2298..2888
                     /locus_tag="VF357_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7522572.1"
                     /translation="MRKPLMIAGVMLLCCGVAFGAQTARKGGKPMVILETSLGVIKVE
                     LYPDKAPVSVKNFLAYVKEGHYDGLIFHRVIRDFMVQGGGFTKEMQERGSKHPPIKNE
                     ADNGLKNDRGTLAMARTSVVDSATAQFFINVVNNDFLNHRAKTPQGYGYAVFGKVTEG
                     MDVVDKIRAVPTGNSGMFQDVPLQPVAITKATVVQE"
     gene            complement(2896..3381)
                     /locus_tag="VF357_00100"
     CDS             complement(2896..3381)
                     /locus_tag="VF357_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7522573.1"
                     /translation="MYEKILVPLDGSALAERAIRHAQEIARGTRSEILLLQAVNFPMP
                     VVPEAVLVPDGKWLDEARKEAARYLEGIAAPLREAGMRVRTLLDDRPPADAILHVATR
                     EEVDLIVMSTHGRGGFSRLLMGSVAETVFRATSRTVMLVKPEQPAVVTQHDEELYLHI
                     P"
     gene            3422..4327
                     /locus_tag="VF357_00105"
     CDS             3422..4327
                     /locus_tag="VF357_00105"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7522574.1"
                     /translation="MLILQSLGVLPGESGVRLDRFLRDRFPGVPSRSVRFAIEGGTVR
                     VGGAASPKGRILSEGEIVTVASIAEGSDWLPVPSDLPGASVVYEDSHVAVLCKPHDAH
                     TEPQRPLEAGTLAGYLRKLHPEVEEIAPVPGLTLLTRLDFATSGAVPAALTTQAWVFL
                     QHEREMGAIGKRYLCLVEGDVPESIPIDYAIDSGGGGTVRVRKESADPDPCRWTWIEP
                     VRGVGAGRTLVRATISRGKRHQIRAHLAAAGHPIVGDRRYAAVPPEGPGKARLMLHAE
                     EVTFRHPATGEPFRVVCPPPECFNS"
     gene            complement(4342..>4645)
                     /locus_tag="VF357_00110"
     CDS             complement(4342..>4645)
                     /locus_tag="VF357_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522575.1"
                     /translation="APSSPPGPSVSPRPSNGERVDRPERTQDDNATLAEKLRAKIDAK
                     DRFLRAVDEKQSLATNPLRNRFVSPSDLELYRKYKEELPADRERLKALETRLPPVK"
BASE COUNT          747 a         1459 c         1605 g          834 t
ORIGIN      
        1 ctgcatcttc gtcaggtcct gggcgatgga gaaaagggtc ggggtctgga aactggcggc
       61 caccgtctgc ccgacgtcga cgttgcgaga gatgacggtt ccctcgacag gcgagcggat
      121 cgtggcgtac tcgaggttcg tctgagcgac cttgagcgcc cccagggcct gcgtcacccc
      181 ggcctctgcg cttttttgcg ccgcgactgc ctggtcatag gcggtctgcg ccgcgtcgac
      241 gtccgactgt gcgacgaagc cgttgggaag aagctcccgg ttccggcggt acgtacgctc
      301 cgtgtccgtg acggagacct tcgccttctc cagggagcct tttgcgttct cgaggctccc
      361 cttcgcctgg atgagcgagg cttcgagcag ctgggggtcg atctgggcga tcacctcgtt
      421 ctttcgcacg cgggaattgt agtcgacgaa gagcttccgg atcgtccccg agatctggct
      481 tccgacggaa acggtggtca cggcgttgat gttccccgtc gcggtgatgg tgtcgacgat
      541 atccccccgc tcgaccttcg cggtccggta gttgggcgtg ccgttatcct tcttcaagta
      601 gaggtacgcc gcgccggcga gtgccgccgc caggacgacc ccgatcagga gtttcctcat
      661 ggagttccct tccgttccct ggatgcgcta atgtttccct ttagcaggat cccctgccgg
      721 tcgagcagtt ccccggtggc cttccacagg ttcgtgaacg cggactggta atccgcgcgc
      781 gccgccgtgt agttctcccg cgcctgtgtc aggctggcgg agacgtcaag gacgtccttg
      841 gtggtggcca gccccagctg ttgccgcttc agatacgaat ccatgaggac ctccgcggcc
      901 gtcaccccct gttcggtcac tccgagctgg tcctgcgcag aagtcagggc gcgcagggcg
      961 gtccggacct cgagggtcgc cgactcctcg gtcgccgcga gctgggttcg ggcctggctt
     1021 gcgatcagcc ggctcgccgc gacgtcggcg cgggcggagt tgttcccgat ggggacggag
     1081 aagctcaatc ccaccgacca gatggggtat ctggcgctgc ccatgtcgcc gaacgcgtcc
     1141 ccgtagctgg agtcgagccc cgtgatcccg ctgcttccct gcagcgcaag ggagggaaga
     1201 gcgaggttgc gggatacctt ctcgttgaat tccgcggtgc ggacggcaac gcgcgccttc
     1261 acgatatcgg ggcgccgctg catcgcggtg cgcaccgcgt cgggttcgga cacttccgac
     1321 gtctcgggag gcggcggcgt ggtcgcgacg gccgtgcccg cctcaaggtg cagtattgtg
     1381 agaagctggt cggcggcgtc catctccgcg cgcttcgcgt ccaggaggtt cttctgtgcc
     1441 tgaagtaccc ccagccggga atcctgcaac tggtaggcgg ccagcactcc ggcgtcgaca
     1501 cgcccctcgt tctgcgcctg gacctcgcgg gcgagggcca gggaggtctg ccgcgtcgcg
     1561 agggactccc gcgtcttcat gaggaccaga tactggttcc tcgcaagcgc ggcggtgttc
     1621 agcgcctgct gggtccaatc cgcgagggcg gaatccgtcg cgtcgtccgc cgtggtgatg
     1681 ccgcgctccg tcacctcacg gcccagtccc tggaggagcg gttgggagaa cgacagggtc
     1741 agctgcggct gagcgaaacg ggaggtcgga aggcccaggt tgtccctgga ccagaaattg
     1801 ttgaaagaga gcgacgtcgt ggcgcctgag gggagcaact gcgagatcga cgcgtcgaac
     1861 tggaaatcgc gttgccggtc gacgaagaat gattgcgggg ccgcttgccg gttcgatccc
     1921 tggtagtcaa ccagggccga aaggagcgga tcgtagatcg cgcgcgcctt ccggataccg
     1981 gtttcggcga tggcggggga gtaggtcgcc acctgcagcc ccaggttctt ctccacggct
     2041 atccgcaccg cgtccccgat cgacagcgaa agcgtttcgg cgccggcggc ggagaagggg
     2101 tacgcgcaaa ggagtacgag gagaagcggc agcgcacgca gcaaaggggt cattctggga
     2161 ggcgtcatcc ggtacattat aatcataagg gcgcgggaaa cggcccaaag gtttcatcct
     2221 gcccgtatga acgcttccac cgtgcgggcg attcgaatac aataaaacat caatcacaaa
     2281 ggaatcgggg gtcttcgatg cggaaaccgt tgatgatcgc gggagttatg ctcctctgct
     2341 gcggggtcgc cttcggcgca cagaccgccc gcaaaggagg caagcccatg gtaatcctgg
     2401 aaacaagtct cggcgtgatc aaggttgaat tgtaccccga caaggcgccc gtttccgtga
     2461 agaactttct cgcctacgtc aaggaaggtc actacgacgg gttgatcttt caccgggtga
     2521 tccgggattt catggtccag ggcgggggat tcacaaagga gatgcaggag cgcggatcga
     2581 agcacccgcc gatcaaaaac gaggcggaca acgggctcaa gaacgaccgc ggcacgctgg
     2641 cgatggcccg gacctcggtc gtcgattccg cgacggcgca attcttcatc aacgtggtga
     2701 acaacgactt cctcaaccat cgggcgaaga cccctcaagg gtacggctac gccgtgttcg
     2761 gcaaggtcac ggaggggatg gacgtggtcg acaagatacg tgccgtgccc accgggaact
     2821 ccggcatgtt ccaggacgtt cccttgcagc ccgtcgccat cacgaaggcg acggtcgtgc
     2881 aagagtgagg ggacgctacg gaatatggag gtacagctcc tcgtcgtgtt gtgtcacgac
     2941 ggcgggctgc tcgggcttca cgagcattac cgtccgagag gtcgcccgga acacggtctc
     3001 cgcgacgctt cccatcagca ggcgggagaa cccgccgcgt ccgtgcgtgc tcatgacgat
     3061 caggtcgacc tcctcgcgcg tggccacgtg aaggatcgcg tcggcggggg gccgatcgtc
     3121 cagcagcgtg cggacgcgca tccccgcctc gcgcagcggc gcggcgatcc cttcgaggta
     3181 ccgggcggcc tccttcctcg cctcgtcgag ccatttgccg tcggggacca ggacggcttc
     3241 ggggacgacg ggcatcggga agttgacggc ctgcagcagg aggatttcgc ttcgggtacc
     3301 ccgggcgatc tcctgcgcat gccggatcgc ccgctccgcg agggcggagc cgtccagcgg
     3361 gacgaggatc ttctcgtaca tggcggaccc tcctttcttt gtatacagta tacaccgtga
     3421 gatgctgatc ctccagtcac tcggcgttct ccccggggag tccggggtcc gtctcgaccg
     3481 gtttctgcgg gaccggttcc cgggggtccc ttcgcgaagc gtgcggtttg ccatcgaggg
     3541 gggaacggtc cgggtcggcg gcgccgcctc ccccaagggg cgcatcctca gcgaaggcga
     3601 gatcgtcacc gtggcgtcca tcgccgaggg atcggactgg ctccccgtcc cttccgatct
     3661 ccccggggcg tccgtcgtct atgaagattc ccacgtcgcc gtcctgtgca agccgcatga
     3721 cgcccacacc gagccccagc ggccgctgga agcggggacg cttgccggct acctgaggaa
     3781 gcttcacccg gaggtggagg aaatcgcccc ggtcccgggc ctcacgctgc tgacgcgcct
     3841 cgatttcgcc accagcgggg cggttccggc ggcgctcacg acgcaggcgt gggtatttct
     3901 gcagcacgag cgggagatgg gggcgatcgg gaagcggtac ctttgccttg tggaggggga
     3961 cgtgccggag tcgatcccga tcgactacgc gatcgattcg ggcggggggg ggaccgtccg
     4021 ggtgcggaag gaatcggcgg acccggaccc atgccggtgg acctggatcg agcctgtgag
     4081 gggcgtcggt gccggccgca ccctggtccg ggcgacgatc tcccgcggca agcggcacca
     4141 gatccgggcg cacctcgccg cggccggcca tccgatcgtg ggagaccgcc ggtacgccgc
     4201 cgtcccgccg gaggggcccg ggaaggcgcg gttgatgctg catgcggagg aggtcacgtt
     4261 ccggcacccc gccacggggg aaccgtttcg ggtcgtctgc ccgcccccgg agtgcttcaa
     4321 ttcataaata cggtgctacg gctacttgac gggggggagg cgggtctcga gcgccttcag
     4381 acgctcccgg tcggcgggga gttcctcctt gtacttccgg tagagctcga ggtcggaggg
     4441 ggagacgaag cggttccgta acgggttggt agcgagggac tgcttctcat ccacggcgcg
     4501 aaggaaccgg tccttcgcgt cgatcttcgc gcgaagtttt tccgccagcg tcgcgttatc
     4561 gtcctgcgta cgctccgggc gatcgaccct ctctccgttc gacggcctcg gggagacgga
     4621 cgggcccggc ggcgaggagg gggcg
//
LOCUS       DASVJL010000008         4070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_94897, whole genome shotgun sequence.
ACCESSION   DASVJL010000008 DASVJL010000000
VERSION     DASVJL010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94897"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VF357_00115"
     CDS             complement(<1..759)
                     /locus_tag="VF357_00115"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX7522576.1"
                     /translation="MQKASKLIVVNAAPYETRVATLESGILVELLIERGEDRNSVGNI
                     YNGKVIRVLPGMQAAFVDIGMDKAGFLFAGDFVTPQLEFDTDPADEPVLPEEIGIRPA
                     RFPQDTFVPPIEGLIREGQHLLVQVAKEPLGTKGARITSHITLPGRHLVLLTWSAHIG
                     ISRRIEDPEERDRLSKIVETSRPEGMGAIVRTAAEGRSEAELKADMDYLVRLWETIRK
                     RSESAAAPAMIHRELSLSLRAVRDLFSSESDRIAV"
     gene            complement(764..3256)
                     /locus_tag="VF357_00120"
     CDS             complement(764..3256)
                     /locus_tag="VF357_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HEX7522577.1"
                     /translation="MREIPPSIRKPSRYCGGEVRPPSIPWDDASVRVLLAFPDVYEIG
                     MSHLGILLLREILSARPGTLCDRVFAPWTDYEEHLRAAGLPLASLESGRPAGAFDLLG
                     FSLCYELTYTNVLAMLDLSAIPLLSKDRSEEHPLVVAGGVCTLNPAPVAPFFDALLVG
                     DGEEVVLEIVALVEEWKKEGGSRADLLTRLSRIEGVYVPGISRRVARRVLADLNRSPL
                     LPAPILPAMRVVHDRLSVEISRGCTRGCRFCQAGYAYRPVRERDPLALLRYLQEEAPK
                     TGYDEVGLLSLSAADYSCVDRLITEAMEVLAPSSVSLSLPSLRLDALRENTVRQIRKV
                     RKSGFTLAPEAGTSLLRRTLNKEIPDDDVLKTAEWIFGNGWQTLKLYFMVGLPGETAD
                     DVRAIGALAGRVAAIARRHGKRTSVTVSVSAFVPKPHTPFQWERQIGREEIQERIRLL
                     RDALGRNRHAELKFHSPETSALEGVFSRGDDRLPGVIARAFRNGARFDAWTEAFRPDA
                     WKRAFEEEGVDPLEYLRERDPRDPLPWEFVDAGIDREFLLSEREKARAGETTPDCRAA
                     GCSSCGACPPGLSNITYPGRLGEPVAADEEGERVSHPAATSAQRHVVRLSYAKEGPAK
                     YLSGLEIQSLWGRVFRRAGLPLAYSQGFNPSPKLSLSPALAVGAESDVEFLEAEFTLP
                     VPAGDVPVKLAPHLPAGIRVTFASSVPPGSPRLSDFDIASDWSLRPVPPFSLPEEATP
                     GRAAERLAAFRASDRHPLVLTREDRTSEIDLKPIVQTFGVNPDGISITIIQGTGKGVR
                     PLEAAASLLGVPLSPERFLPRKVSAELIPRRG"
     gene            3334..>4070
                     /locus_tag="VF357_00125"
     CDS             3334..>4070
                     /locus_tag="VF357_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7522578.1"
                     /translation="MRLFGTMGAEASFREEDFFGREEELSALTRAALEGSRGIGSSFM
                     IYGPPNIGKTSLLLKLAKMLGASVGGEGLPRPFPLYFSFSQILSHPLALSQHFLQEFL
                     SQLLRFLGDARPSAFDPAAMCDRLASFGVTGCREVLAAHERCTAEGDGLSALVNALSF
                     PFSASGELFYPVFLFDDFQYTGKLQGLPEGAMLSILRPYIKAGHFPMFLSGSSPGRVT
                     AALKREGLFGTFQMIEVGGLSTDSSVRL"
BASE COUNT          630 a         1392 c         1378 g          670 t
ORIGIN      
        1 gacggcgatc cggtcgctct ccgaggagaa caggtcccgc acggcgcgca gggaaaggga
       61 gagctccctg tggatcatgg cgggagccgc ggcgctttcg ctcctcttcc ggatcgtctc
      121 ccagagccgg acgaggtaat ccatgtcggc cttgagctcc gcctccgacc gcccctcggc
      181 ggccgtgcgc acgatcgcgc ccatcccctc gggacggctc gtctccacga tcttcgacag
      241 ccggtcccgc tcctccgggt cctcgatccg acgggagatc ccgatgtggg ccgaccacgt
      301 cagaagcacc aggtgacgcc ctggtagggt gatgtggctg gtgatcctcg cccctttggt
      361 cccgagcggc tccttggcca cctggacgag caggtgctgc ccctcccgga taagcccctc
      421 gatcggggga acgaacgtgt cctgggggaa gcgtgccggc cggattccga tctcctcggg
      481 caggaccggc tcatcggccg ggtcggtgtc gaactcgagc tgaggcgtga cgaagtcgcc
      541 cgcgaagagg aaccccgcct tgtccatccc gatgtcgacg aaggcggcct gcatccccgg
      601 gaggacgcgg atgacttttc cgttgtagat gttgccgacg gagttccggt cctcgccccg
      661 ctcgatcagc aactccacga ggatcccgga ttcgagcgtc gccacgcgcg tctcgtacgg
      721 cgcggcgttc accacgatca gcttgctggc cttctgcatc gcgctacccg cggcggggga
      781 tcagctccgc cgacaccttt cttggtagga accgttcggg ggacagcggc acccccagaa
      841 gggaggcggc ggcctcgagc ggccggacgc cctttcctgt gccttggatg attgtaatgg
      901 agatgccgtc cgggttcacc ccgaacgttt gaacgatcgg cttcaggtcg atctcggagg
      961 tccggtcctc ccgcgtgagg acgagggggt gccggtccga cgcgcggaag gcggcgaggc
     1021 gctccgcggc ccgccccggc gtggcctctt cgggcaggga aaacggcggg acgggacgca
     1081 gggaccagtc ggaggcgatg tcgaagtccg acagtcgcgg tgagcccggc gggacgctcg
     1141 aagcaaaggt gacgcggatc cccgcgggaa ggtgcggcgc aagttttacc ggcacatccc
     1201 ccgccgggac ggggagggtg aactcggcct cgaggaattc gacgtcgctc tccgccccca
     1261 cggcgagggc gggggagagg gagagtttcg gcgaagggtt gaacccctgg ctgtacgcca
     1321 acggcaggcc ggcccgccgg aagacgcgtc cccacaacga ctggatctcc aggccgctca
     1381 agtatttcgc gggaccctcc ttggcgtagg agaggcggac gacgtggcgc tgcgcgctgg
     1441 tcgccgccgg gtgcgaaaca cgttcgccct cctcgtccgc cgccacgggt tccccgagcc
     1501 gtccggggta cgtgatgttc gacagacccg gggggcacgc cccgcaggag gagcagcccg
     1561 ccgcgcggca atcgggcgtc gtttcccccg cgcgggcctt ctcccgctcg gagaggagaa
     1621 actcccgatc gatcccggcg tccacgaact cccacgggag cggatcccgc gggtcgcgtt
     1681 cccgcaggta ttcgagcggg tcgacccctt cctcctcgaa cgcccgcttc cacgcgtcgg
     1741 gccggaacgc ctccgtccac gcgtcgaacc gcgcgccgtt ccggaacgcc cgtgcaatca
     1801 ctcccggcag ccggtcgtcc ccccgcgaga agactccctc gagcgcggag gtctccgggg
     1861 agtggaactt cagctctgcg tggcggttcc gccccaacgc gtcccggagc aggcgaatcc
     1921 gctcctggat ctcctcgcgg ccgatctggc gctcccactg gaacggcgtg tgcggcttcg
     1981 ggacgaaggc ggagacgctc accgtcacgg aggtgcgctt cccgtggcga cgcgcgatcg
     2041 cggcgacccg gccggccagt gccccgatgg cccggacgtc gtccgccgtt tccccgggca
     2101 ggccgaccat gaagtagagc ttgagggtct gccagccgtt gccgaagatc cactcggccg
     2161 tcttcaggac gtcgtcgtcc gggatctcct tgttgagcgt tcggcgcagc agcgaagtcc
     2221 ccgcttccgg cgccagcgtg aagcccgact tgcgaacctt ccggatctgc cggaccgtgt
     2281 tctcacgcaa cgcgtccagg cgaagcgagg ggagggagag ggatacgctc gaaggcgcga
     2341 gcacctccat cgcctccgtg atgagccggt cgacgcagct gtagtcggcg gcggacagcg
     2401 acaggagccc cacctcgtcg tatcccgtct tgggcgcctc ctcctgaagg taccgaagca
     2461 gggcgagcgg gtcgcgctcc ctcaccggcc gataagcgta tcccgcctgg cagaagcggc
     2521 accccctcgt gcacccccgg gagatctcga cgctcaggcg gtcgtggacc acgcgcatcg
     2581 ccggcaggat cggcgccggc agcagggggg aacggttgag gtccgcaagg acgcgcctcg
     2641 ccacccgccg ggagatcccc gggacgtaca ctccctcgat ccgggagagc cgtgtgagga
     2701 ggtccgcgcg ggatccgccc tcttttttcc attcctcgac gagtgcgacg atctcgagca
     2761 ccacttcctc gccgtccccg acgaggaggg cgtcgaagaa gggggccacc ggagcggggt
     2821 tcaaggtgca cacgcccccg gccaccacga gggggtgctc ctccgatcgg tctttcgaca
     2881 ggagggggat cgcggaaagg tcgagcatcg cgaggacgtt cgtgtaggtc agttcgtagc
     2941 acagggagaa tccgaggagg tcgaacgcac cggcgggtcg ccccgattcg agcgatgcga
     3001 gcggcagccc ggcggcgcgc aggtgctcct cgtagtcggt ccagggggcg aagacgcgat
     3061 cgcaaagggt cccggggcgt gcggagagga tctcgcgcag gagcaggatc ccgaggtgcg
     3121 acatcccgat ctcgtacacg tcgggaaaag cgagcagcac gcgcacgctc gcgtcgtccc
     3181 acgggatgga gggaggacgg acttcgcctc cgcaatatcg cgacggcttc cggatggagg
     3241 gggggatttc gcgcatgtcc ttcagaaaat cccattatat ttttgaaaac gcaacggatt
     3301 cacgttacat tatcccgagg ggggataggg taattgcgtc ttttcgggac catgggggcg
     3361 gaagcgtcct ttcgggaaga agattttttc gggcgcgagg aagaactctc cgccctgacg
     3421 cgcgcggcgt tggaggggag ccggggcatc ggctcctcct tcatgatcta cggacccccc
     3481 aacatcggca agacgtcgct gctcctcaag ctggcgaaga tgctgggggc atccgtcggc
     3541 ggagagggac ttccccgccc gttcccgctc tacttttcct tcagccagat cttgtcgcac
     3601 ccgctggcgc tctcccagca tttcctgcag gagttcctct cccaattgct ccggttcctc
     3661 ggggacgcgc gaccttcggc cttcgacccc gcggcgatgt gcgaccgcct ggcctccttc
     3721 ggcgtcacgg ggtgccggga ggttctcgcc gcccacgagc ggtgcaccgc cgagggggac
     3781 ggcctctccg ctctcgtgaa cgccctctcg ttcccgttct cggcgtcggg ggagctcttc
     3841 tatccggtgt tcctcttcga cgacttccag tatacgggga aactccaggg cttgcccgag
     3901 ggggcgatgc tttccatcct tcgcccctac atcaaggccg gccacttccc gatgttcctc
     3961 tcgggctcct cgcccggccg cgtcaccgcg gccctcaagc gcgaggggct cttcggcacc
     4021 ttccagatga tcgaagtggg cgggctctcg accgactcct ccgtccgcct
//
LOCUS       DASVJL010000009         6730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1182, whole genome shotgun sequence.
ACCESSION   DASVJL010000009 DASVJL010000000
VERSION     DASVJL010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="VF357_00130"
     CDS             <1..1397
                     /locus_tag="VF357_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7522579.1"
                     /translation="DIRTAPLPLLAFLLACAVAPFLPRIGFFLPIVGRGKPGAKGVAL
                     TFDDGPDPEVTPLLLDLLDRHSVSATFFVTGERAARHPSIIRDILSRGHTIGNHSYHH
                     FPFLMLKGIRTLRREIESTQSCLAGYGILPLAFRPPAGITNPLLRRVLLEQGMYCVNY
                     SCRAVDIGNRRIRRLSEKVLKAVSRGDIIALHDVAPRHAGRERLMAEFDALLGGLKEK
                     GLEVVPLDRLTGREVMRRGGSPGEGHPAALFFDGLAPDYDREQFRFPVSIVRKAEYAL
                     FEARLPSLFSTSDRVLEIGAGTGIFTLAIARRCREVTAVDISVSMLEILKGKAAGMGL
                     DNIRTVVGNAETMDLDGSYTVACAFSSLEYLTDHQAFFRRLVDHVEPGGTLYFTTARR
                     SLFRLFAQIGNAFRQGIWLKAHNRREIEAILSPSGFEEIRIGSHLFKSWLSGGILLEA
                     VARRRIVTDTSAGH"
     gene            1412..2617
                     /locus_tag="VF357_00135"
     CDS             1412..2617
                     /locus_tag="VF357_00135"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX7522580.1"
                     /translation="MGTPLPKTLLVIPVHNHAATLRAVALKGLAEGFPVLVVDDGSTD
                     GGLDAVVDLPVARHRFPWKRGKGAAILAAAELAKRSEYDAIVTIDADGRHDPADARRL
                     LEAVSASWPAVAIGARRMEDANAPPSGVFPRDFSNFLVRLECGRALHDSRSGYRLYPV
                     DFLLAGRFLSAGIPFEVEVLVRAGWAGLPLLSVPVPGSSPSAGERISHFHLFLDNLRL
                     FCLHAWLVARSLLPWPHRRRSPREDVGPKTTEFLHPVRFFRRLSREHSGAGELAAAVW
                     VGIFLGALPILPFGTAAIVYVNHRLHLNKLAGVAASNICCVPFVPLACVEAGYFFRHG
                     RFLFAFDRHVLLDEIPLRLWEWLLGALVIGPILGFAGALLTYFAVRSLRANRSPDARP
                     TGNRERMRQ"
     gene            complement(2611..2793)
                     /locus_tag="VF357_00140"
     CDS             complement(2611..2793)
                     /locus_tag="VF357_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522581.1"
                     /translation="MAVSAAVAAGVLSGLFLFVLWAGWGGAAGLLVAAGIFLVWFYRG
                     RRPDLETGKARRDRFH"
     gene            complement(2822..3655)
                     /locus_tag="VF357_00145"
     CDS             complement(2822..3655)
                     /locus_tag="VF357_00145"
                     /inference="COORDINATES: protein motif:HMM:NF023385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7522582.1"
                     /translation="MTTCRNALTVDLEDWYHICGTGQSGDVSTWDAYESRVTRNTAKV
                     LSLLRKHRARATFFVLGYIAEREPELIAEIAKEGHEIATHGHHHRRVFEMSEEEFAED
                     VDRSMEAISSAGGGRVIGYRAPEWSIRPHTMWALRVLRRKGIRYDSSMVPLTRMGDRS
                     FPTAPCRIPTEDGEILEFPLTTVRCFGEHLPFTGGLPLRLMPYFYVLSRIRRMNAGGD
                     AAMVYVHPWEFDAGQPRIELPWSRRIMHYFNLRSTPGKLSGLLVNLRFAPLREVLGVG
                     H"
     gene            complement(3665..4834)
                     /locus_tag="VF357_00150"
     CDS             complement(3665..4834)
                     /locus_tag="VF357_00150"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7522583.1"
                     /translation="MPSSMPAVLLLDGLWNKTVAAARSLGKRGYRVSVGERTRFAPAL
                     FSRYCSRRFLHPSPATSPEAFLDALEKELRVGGYDVVLAMEFGTQVLIARNRPRFEGM
                     ARFPFASADLAIRVQDKGELASFASVHGVECPATFRPEGPGDVHAMASLLPYPLLIKP
                     RHSSGGRGIVRVGTPSQLRKEYPKVHAVHPSPILQECLPPGGAALGVGVLMNFSSEPR
                     ATFAYRRLREFPVSGGPSTLRESVRDEPLCRTTERLLSALQWTGVAMAEFKVDPRDGR
                     PKLLEVNPRFWGSLNHAILSGVDFPHLLCRMAIDGDVPPLEEYGVGVRSRSLIHGDLM
                     HFVRNPRRFHLRPRFFDFSIPDDLLSASDPWPVVGRVATLIPACYDRELRKTMFG"
     gene            complement(4927..5700)
                     /locus_tag="VF357_00155"
     CDS             complement(4927..5700)
                     /locus_tag="VF357_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX7522584.1"
                     /translation="MLLLEVARSASVSRGTLRWIVPALILALCLAPRPNLAEETVPGG
                     KKISGAERDRWFEEVRARQKGITGLSAAVVQRKRDPLLKNEAVSRGKLSFRKPASLRW
                     EVESPERLVVVMDGRTVTTYYPARKEAERRDMQDDFASRSALGFIESGISASLPELER
                     RFHVDLFLSDGQVILQLIPRSKMLSRTIASIRIHEDPVEGRPRRIVVEGARGDRTETT
                     FSGVTLNPEFPPDTFSLRLGPEVRVTEPGEPERNRVDAP"
     gene            complement(5687..>6730)
                     /locus_tag="VF357_00160"
     CDS             complement(5687..>6730)
                     /locus_tag="VF357_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7522585.1"
                     /translation="GGEPTLHPRFLDIVAEARKMGFSHLQAATNGIRFADPEFARRAR
                     DAGLQYLYLQMDGTTDDVFLKIRGKALLDTKLRVIDSARKAGLRVIFVPTIVKGVNDH
                     QIGDLCRLAFDHLDVLSGISFQPMTFIGRYPESEREKLRYTLSDLSREFSRQTGFTHP
                     LEDWFPLNAAVPLVRYAGAVSGNVSVNHACHPHCGLMTLLFVGRNREAVPITRFLDLR
                     GLLGEVERLTGSARTSRVKSLSKIRALQAFYRFFREDRAPDGLTFLRFLKTLDGFAEK
                     KYSWDEKYEGHTYKTFYVLGMHFMDAYNYDLERVSRCGVHYSAPDGRIYPFCTYNSGP
                     VHRHRVENSIASS"
BASE COUNT         1175 a         2282 c         2079 g         1194 t
ORIGIN      
        1 tcgacatccg cacggcgccg ctcccgttgc tcgccttcct cctcgcctgc gccgtcgcgc
       61 cgttcctgcc ccgcatcggc ttcttcctcc cgatcgtcgg cagggggaaa ccgggggcga
      121 agggcgtggc cctcacgttc gacgacgggc cggaccccga ggtcacccct ctcctcctgg
      181 accttctcga caggcactcg gtttcggcga cgttcttcgt gacgggggag cgggcggccc
      241 gtcatccctc catcatccgg gacatcctgt ctcgtggtca cacgatcgga aaccactcct
      301 accaccactt cccgttcctg atgctgaaag ggatccggac actccggcgg gagatcgaat
      361 cgacccaatc ctgcctcgcc gggtacggca tcctccccct ggcgttccgg ccccccgccg
      421 ggatcacgaa tccgctcctc cggcgggtcc tcctagagca ggggatgtat tgcgtcaact
      481 acagctgccg cgccgtcgac atcgggaacc ggcggatccg gcgcctctcc gaaaaagtgc
      541 tgaaggcggt ctcccggggc gacatcatcg cgctccacga cgttgcgccg cgacacgccg
      601 ggagggagcg cctcatggcc gaattcgacg ccctgctcgg cggattgaag gagaagggtc
      661 tggaggtcgt tcccctcgac cgcctgaccg gcagagaggt catgcggagg ggaggatcgc
      721 ccggcgaggg gcatccggcg gcgctttttt tcgacgggtt ggcgcccgac tacgaccgtg
      781 agcagttccg tttccccgtc tcgatcgtcc gcaaggccga atacgcgctc ttcgaagcgc
      841 ggctcccgtc gctcttttcc acgtcggacc gggtgcttga gatcggcgcc ggcaccggca
      901 tcttcaccct ggcgatcgcc cgacgttgcc gggaggtgac ggccgtcgac atctccgtga
      961 gcatgctgga gatattgaaa gggaaagccg ccgggatggg gctcgacaac atccggacgg
     1021 tcgtgggaaa cgcggaaacg atggacctcg atggatccta tacggttgcc tgcgccttct
     1081 cctccctcga atacctaacg gaccatcagg ccttttttcg ccggctcgtg gatcacgtcg
     1141 aaccgggagg aacgctgtat ttcaccaccg cccgacggtc cctgttccgt cttttcgcgc
     1201 agatcggcaa tgcgtttcgg caagggatat ggctgaaagc ccacaaccgg agggaaatcg
     1261 aggccatcct gtccccttcg gggttcgagg agatccggat cggctcccac ctcttcaaat
     1321 cctggttgtc cggagggatc ctcctggaag cggtggcccg ccgccgcatc gtcacggaca
     1381 cctcggccgg ccactgatgt agaatcgccc catgggaacg ccgttaccga agacgttgct
     1441 cgtcatcccg gtccacaacc acgccgcgac cttgcgcgcc gtcgcgctga aaggcctggc
     1501 cgaagggttc cccgttctcg tcgtcgatga cgggagcacg gacggcgggc tcgacgcggt
     1561 ggtcgacctt ccggtcgccc gtcaccggtt cccgtggaaa cgggggaaag gagccgccat
     1621 cctcgccgcg gcggagttgg cgaaacggtc ggaatacgat gcgatcgtga cgatcgatgc
     1681 cgacggccgc cacgacccgg cggacgcccg ccgtctcctg gaagccgtct ccgcttcctg
     1741 gccggccgtc gcgatcggtg cccgccggat ggaagacgcg aacgcccctc cttccggcgt
     1801 gttcccccgc gatttttcga atttcctggt ccggctggaa tgcggccgcg cgcttcacga
     1861 cagccggagc ggctatcggc tgtatccggt cgatttcctc ctcgccggcc gtttcctctc
     1921 cgcgggcatc ccgttcgaag ttgaggtgct ggtccgggcg ggatgggcgg gcttgccgct
     1981 cctctccgtt ccggtcccgg gttcttcccc gtccgccggc gaacggattt cgcatttcca
     2041 cctgttcctc gacaacctgc ggcttttctg cctccacgca tggctcgtgg cccgttccct
     2101 cctgccttgg ccgcaccgcc ggcgatcgcc cagggaggac gttggcccga agacgacgga
     2161 gttccttcat ccggtccgct ttttccggcg gttgagcagg gagcactccg gagcggggga
     2221 actggccgcc gccgtgtggg tcgggatctt tctcggggcg ctcccgatcc tcccgttcgg
     2281 gaccgcggcg atcgtctacg tcaaccaccg ccttcacctg aacaagctgg ccggagtcgc
     2341 cgccagcaac atctgctgtg tccccttcgt cccactcgcc tgcgtcgagg ccggttattt
     2401 cttccggcac ggccgttttc tgttcgcatt cgatcggcac gttctcctcg atgagatccc
     2461 cctccgcctg tgggagtggc tgctgggagc gctcgtcatc ggtccgatcc tcggcttcgc
     2521 aggagcgttg ctcacctact tcgcggtccg atccctgcgg gcgaaccggt ccccggacgc
     2581 ccggccgaca ggaaaccggg agcgaatgcg tcagtgaaat cgatcgcggc gagccttccc
     2641 cgtctccagg tcgggccgcc ttcctctgta gaaccagacg aggaaaatcc ccgcggccac
     2701 caggagaccg gccgccccgc cccatcccgc ccacaaaacg aagagaaaga gcccggaaag
     2761 aacccccgcg gccaccgccg cagaaacggc catcgagatc gaatatcgcg gctcccgaac
     2821 gttaatgacc gaccccgagc acttcgcgaa ggggggcgaa tcgcaggttg acgagcagtc
     2881 cggaaagctt cccgggggtc gaacgcaggt tgaaataatg catgatccgc cggctccatg
     2941 gaagttcgat cctcggctgc cccgcgtcga attcccacgg gtggacgtac accatcgccg
     3001 catccccccc tgcattcatc cggcggatcc tcgagagaac gtagaaatac ggcatgaggc
     3061 gcaacggcaa cccccccgtg aaggggagat gttccccgaa acagcgtacg gtggtcaagg
     3121 ggaactcgag gatctccccg tcctccgtcg gaatccggca gggcgccgtc gggaaggagc
     3181 ggtcccccat tcgcgtcaag gggaccatgc tggagtcgta ccggatccct ttccgccgca
     3241 agacccgaag cgcccacatc gtgtggggcc ggatcgacca ttctggtgcg cggtacccga
     3301 tgacgcgccc cccccctgcg gacgatatcg cttccatcga acggtcgacg tcctccgcga
     3361 actcttcctc ggacatctcg aacaccctcc ggtggtgatg tccgtgggtg gcgatctcgt
     3421 ggccttcctt cgcgatttcg gcgatcaact ccggttcgcg ttcggcgatg tatccgagga
     3481 cgaaaaacgt ggcacgggcc cgatgtttcc gcagaaggga aaggaccttc gccgtgttgc
     3541 gggtcacgcg actctcgtac gcgtcccacg tggagacgtc cccggactgg cccgtgccgc
     3601 agatgtgata ccagtcttcc aggtccacgg tcagcgcgtt ccggcaggtc gtcacccttc
     3661 gctcctatcc gaacatcgtt ttccgcaact cccggtcgta gcacgcaggg atcagggttg
     3721 ccacccgccc caccacgggc cacggatccg atgccgaaag cagatcatcc gggatcgaga
     3781 aatcgaagaa gcgagggcgg aggtggaacc gtcgcgggtt tctcacgaaa tgcatcagat
     3841 ccccatggat cagggatcga ctccgaacgc ccaccccgta ctcctccagg ggaggaacat
     3901 cgccgtcgat cgccatccgg cacaacaggt gcgggaaatc gacgcccgac aggatcgcat
     3961 ggttcagcga tccccagaac cggggattca cctcgaggag cttcggcctg ccgtccctcg
     4021 ggtcaacctt gaactccgcc atcgcaacgc cggtccactg cagcgccgag aggagccgct
     4081 ccgtcgtccg gcagagcggc tcgtcccgta cgctctcccg gagggtgctg gggcccccgc
     4141 ttacggggaa ttcccgcagg cgccggtagg cgaacgttgc gcgtggttcg gaagaaaagt
     4201 tcatcagcac acccaccccc aacgcggccc ctcccggagg cagacactcc tgaaggatcg
     4261 gagacggatg gacggcatgc actttcggat actctttccg gagttgggag ggagttccca
     4321 cccgcacgat ccctcgcccc ccggaggaat gcctgggctt gatgaggagg gggtatggga
     4381 gaagcgaagc catcgcgtga acgtcccccg gcccttccgg acggaacgtg gcgggacact
     4441 cgacgccgtg gaccgatgcg aacgacgcga gctctccctt gtcctggacc cggatcgcga
     4501 ggtccgcgga cgcgaaaggg aaacgcgcca tcccctcgaa gcgaggacgg ttccgggcga
     4561 tcaggacctg ggtgccgaat tccattgcga ggaccacgtc gtatccgccg acgcgcaact
     4621 ccttttcgag cgcatcgaga aaggcttccg gagaggtcgc cggcgacggg tgaaggaacc
     4681 gacgcgagca gtatctcgaa aacagggcgg gcgcgaaccg tgtcctctcc cccaccgaga
     4741 cgcggtaacc ccgtttcccg agagaacggg cagccgccac cgtcttgttc cagaggccgt
     4801 cgaggagcag gacggccggc atcgagctcg gcatggtcat tcccctgggc cgccgcggtc
     4861 catcaaccct tcagtcgatc gggttcgccc gcgcggtttc ggtcacgggc agcgggagga
     4921 acctcgtcat ggcgcatcca cccggttgcg ttccggttcc cccggttccg tgacccggac
     4981 ctcgggtccc agccgcaagg aaaaggtgtc cggaggaaat tccggattga gggtcacccc
     5041 ggaaaacgtc gtctcggtcc gatccccgcg ggctccctcg accacgattc tacgcggcct
     5101 cccctccacc gggtcctcgt ggatccggat cgatgcgatg gtacgggaca acatcttcga
     5161 gcgcgggatc aactggagga tcacctggcc gtcggacagg aaaaggtcga cgtggaatcg
     5221 tcgttccagt tcgggaaggg aggcggagat cccggattcg ataaaaccca gcgcggagcg
     5281 ggacgcgaaa tcgtcctgca tgtccctccg ctcggcctcc tttctcgccg gataataggt
     5341 ggtcaccgtc cggccatcca tcaccacgac caatctttca gggctctcca cttcccatcg
     5401 gagcgaggca ggcttccgga acgacaactt ccccctggag accgcctcgt ttttcaggag
     5461 cgggtcccgc ttccgctgca ccaccgccgc gctcagtccg gtgatcccct tctggcgcgc
     5521 gcggacctcc tcgaaccagc gatccctctc cgctcccgag atcttctttc cgcccgggac
     5581 ggtttcctcc gccaggttcg gccggggggc aaggcatagc gcaaggatca atgcgggaac
     5641 gatccaccgg agggtcccgc gggagaccga tgcggatcgc gcgacctcaa gaagaagcaa
     5701 tcgaattctc tacccgatga cggtgtacag gtcccgagtt gtacgtgcaa aacgggtaga
     5761 ttcttccgtc gggggccgaa taatgaacgc cgcaacgcga cacgcgctcg agatcgtaat
     5821 tgtaggcatc catgaaatgc attccgagga catagaaggt tttatatgtg tgcccttcgt
     5881 acttttcgtc ccagctgtac ttcttttcgg cgaacccgtc gagggttttg agaaagcgga
     5941 gaaacgtcaa cccgtccgga gcccgatcct cccggaaaaa ccggtaaaaa gcctggaggg
     6001 cacggatctt cgacaggctc ttgacgcggc tcgtcctcgc gctccccgtc agccgttcca
     6061 cctctccgag aagtccccgg agatcgagga acctcgtgat cgggacggcc tcgcggttcc
     6121 ggccgacgaa cagcagggtc atcaacccgc aatggggatg gcacgcgtgg ttcaccgaga
     6181 cgttccccga tacggccccc gcgtatcgga ccaggggaac cgcggcgttc aggggaaacc
     6241 aatcctccag ggggtgggtg aacccggtct ggcggctgaa ctcccgggat agatccgata
     6301 gggtgtatcg aagcttctcg cgctccgatt cggggtacct cccgatgaac gtcatcggct
     6361 ggaaggaaat ccccgacagc acgtcgagat ggtcgaaggc caggcggcag aggtccccga
     6421 tctggtggtc gttcaccccc ttcacgatgg tggggacgaa gatgacgcgg agaccggcct
     6481 tccgcgcgga atcgatcacc cggagtttgg tgtccagcaa cgccttcccg cggatcttca
     6541 ggaagacgtc gtcggtcgtg ccgtccatct gcaggtacag gtattggagc ccggcatccc
     6601 gcgctcttcg cgcgaactcc ggatcggcga accggatccc gttggtggcc gcctggaggt
     6661 gggaaaaccc catcttccgc gcttcggcca cgatatcgag aaaccgcggg tgcagcgtgg
     6721 gctcgccgcc
//
LOCUS       DASVJL010000010         6796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_657900, whole genome shotgun sequence.
ACCESSION   DASVJL010000010 DASVJL010000000
VERSION     DASVJL010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657900"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1057)
                     /gene="tig"
                     /locus_tag="VF357_00165"
     CDS             complement(65..1057)
                     /gene="tig"
                     /locus_tag="VF357_00165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX7522586.1"
                     /translation="MKTSVDAVSGVERKITVEIPADEVDRRIEQEFTELRRMVPVRGF
                     RKGKAPMEMVKRMFRDSVEAEVSEHLVKESLTEVVKEKDLKVLSLPGVEGAKVAPGKD
                     FVFSATVEVVPEVDPVGYKGIPVRREKVSVTDEDVSAAIERLREAFAQFHAVEGRGAA
                     ESDLVEFGFTAVVGGETVDRSDSTAVVLSGGMPYGEEFERRMLGVEPGTTRSFEVAYP
                     ADFRNPKYAGKTVAFEVKVGGVREKKLPEMDDAFAKQFGEVSGVDELKVKLRERLAAE
                     GEERSRHNAEEALRKALSERNPFDVPSTLVKRQTIAMMQDTFQRLSSQGWTLRK"
     gene            complement(1104..1185)
                     /locus_tag="VF357_00170"
     tRNA            complement(1104..1185)
                     /locus_tag="VF357_00170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1150..1152),aa:Leu,seq:tag)
     gene            complement(1264..1659)
                     /locus_tag="VF357_00175"
     CDS             complement(1264..1659)
                     /locus_tag="VF357_00175"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7522587.1"
                     /translation="MTFFRRERRAKERYRPLATVVFTVRSGKDPGRVSSPVTGDAQDV
                     SSTGMSVVTPRLAPDGIHIMYDTLMTFRNRIDATIFPEGKPPVRVQGTVIWFRAADAP
                     PGFYIFGMRFDEEAPSLEELRLAARKNPG"
     gene            complement(1656..3389)
                     /locus_tag="VF357_00180"
     CDS             complement(1656..3389)
                     /locus_tag="VF357_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7522588.1"
                     /translation="MEPSRETRTPVLVVNNDPVVRDMLANLLTEEGFHVALAGSGTEA
                     LKIAANAFFDTVLVELFLPDSSGVEFRRRLARVSPKTRVVVLSSFTTIRNSDDILRFG
                     TSDFILDQKEIVELLRATSAVQPVIAKPVSEDEQLRKCLIDTVDILVSLLEVNDPFFG
                     GNSHITMEYARFVAEEMKLDRETVDEIVVGSLLHDIGRVGIKSDILLGKREISESEFK
                     TVRSHCENGAKIIDAVDFPWKIKPIIIHHHERYDGKGYPSGLKGREIPIGARILAVVD
                     AFTAMTAHRPYRSRSLTREEAIQELHKNIGTQFDPEVVELFTSVVGRKFLFRGLGPKP
                     RILMVDDEIDYLMLLKLKLVNEGFDVAAADNAGDALAAMEKDPPDLVVADVMMPGMDG
                     IAMFRKMREANTPWGDTPLIFVSGKNDSQTKVDALHLGAEDFLIKPVDLKELAARIRN
                     IIRRDAKWRKGASGIAQSAGVMGDLRNLGVPDIVQTLHLGLKTACVRVTGNGAEGKIW
                     FENGRIRHAELGSLSGELAFYEMLRWQEGPFVIAHGQATKLRTIEMDEMQLMMEGLRR
                     LDEEGKEDPTG"
     gene            complement(3391..4929)
                     /gene="gpmI"
                     /locus_tag="VF357_00185"
     CDS             complement(3391..4929)
                     /gene="gpmI"
                     /locus_tag="VF357_00185"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX7522589.1"
                     /translation="MKGPFVALIIMDGWGHREEKEGNAVALADTPFFDRIWAGFPRTL
                     IHASEERVGLPAGQMGNSEVGHLNLGTGRVVYQDLVRISKSVRTGDFFRNPVLCAAMD
                     AAREDGKALHLVGLLSDGGVHSLHTHLYALLRMAKERGVPKVCLHPVLDGRDTPPQSG
                     IDHLRALLAKAAEIGAGEVATVVGRYYTMDRDNRWDRVERAYKAMVRGEGTTSGDPVA
                     AVAASYAAGKTDEFIEPVVIVRDGRPVGRIASGDSVIFFNFRADRAREITRALTQETF
                     DRFPRPERLSLAYACMTPYDETFGLPAAFPTQRLDNILARSLAAAGLTNLRIAETEKY
                     AHVTYFFNGGEETVYPGESRVLIPSPSVSTYDLKPEMSAYEVGERAVAEIASGKHALM
                     VLNFANGDMVGHTGVLPAAIQAIEAVDRNLQRVVEKVWEVGGVALVTADHGNAEQMID
                     PKTGGPFTAHTTNLVPLVLADPRAVGTRLREDRALEDLAPTILDLLTLPVPAEMTGTD
                     VREV"
     gene            complement(4931..6214)
                     /gene="eno"
                     /locus_tag="VF357_00190"
     CDS             complement(4931..6214)
                     /gene="eno"
                     /locus_tag="VF357_00190"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX7522590.1"
                     /translation="MTMIVDVHGREVLDSRGNPTVEVEVLLESGAEGAAIVPSGASTG
                     TREAVELRDGDPKRFMGKGVTKAVRNVNRVIAPKLIGYDATEQVLVDRMLIDLDGTEN
                     KGKLGANAILGASIAVARAAAEACGLPLYRYLGGVGGCTLPVPMMNILNGGSHADNNM
                     DIQEFMVMPVGAKSFSEALRMGVETFHNLKKVLKGKGLNTNVGDEGGFAPQLRSNAEA
                     IEVILEAITKAGYKPGRDICVALDSAASEFREKGKYVFRKSDKSKRDSAQLVRFYEEL
                     CRQYPIVSIEDGFSEDDWEGWKMFTDAMGKKIQIVGDDIFVTNPSILRKGIAQGVANS
                     VLIKLNQIGTVTETIEAIEMAKRAGWTAVVSHRSGETEDSTIADLVVGLSTGQIKTGS
                     ASRTDRIAKYNQLLRIEEELGPAARFDGRGVFYNI"
     gene            complement(6248..>6796)
                     /locus_tag="VF357_00195"
     CDS             complement(6248..>6796)
                     /locus_tag="VF357_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7522591.1"
                     /translation="CVRSGSRLSDRFCAPAEPVSGRGVSIPGVAMFTTILLPSDFSGC
                     SAEAARAARRLAECFGSRLIVLHVLDEPAALDPMFRGEVPLELLRGRMEQYAREGIEA
                     FLKAHFDGLPGVETRTASGVPYREIVREARECGAGLIVIGTHGRTGVERVIFGSTAEK
                     VVRMAPCPVLSVREGGKEFVHP"
BASE COUNT         1075 a         2377 c         2115 g         1229 t
ORIGIN      
        1 ctcacgcgca ccatccgctc cgcgttcggc gcgaaccgct cagccatctt atcggcgtca
       61 aggttcattt tcttaaggtc cacccctggg aggagagccg ctgaaaggta tcctgcatca
      121 tcgcgatcgt ctggcgcttg accagcgtgg aggggacgtc gaacgggttg cgctccgaca
      181 acgccttccg cagggcctcc tccgcgttgt ggcgggagcg ctcctcgcct tccgccgcga
      241 gccgctcccg cagcttcacc ttgagttcgt ccaccccgga cacctcgccg aactgcttcg
      301 cgaacgcgtc gtccatctcg gggagcttct tctcgcgcac accgccgacc ttgacctcga
      361 acgccacggt cttccccgcg tacttcgggt tccggaagtc cgcggggtag gcgacctcga
      421 aggaccgcgt cgtcccgggt tccaccccca gcatccgccg ctcgaactct tcgccgtacg
      481 gcatgccgcc cgagagcacg acggccgtcg agtcgctccg atcgaccgtt tccccaccga
      541 ccacggcggt gaacccgaac tccaccaggt ccgattccgc ggcaccgcga ccctccaccg
      601 cgtggaactg cgcgaacgcc tcccgaagcc gctcgatcgc ggccgagacg tcttcgtcgg
      661 tcacgctcac cttttccctc ctcacgggga tccccttgta gccgaccgga tcgacctcgg
      721 ggacgacctc gacggtggcg gagaagacga aatccttccc gggggcgacc ttcgcgccct
      781 cgacgcccgg cagggaaagg accttgaggt ccttttcctt caccacctcg gtgagcgact
      841 ccttcaccag gtgctccgac acctcggcct cgaccgagtc gcggaacatc cgcttcacca
      901 tctccatcgg agccttcccc ttgcgaaacc cccgcaccgg caccatccgg cgaagctccg
      961 tgaactcctg ctcgatccgg cggtccacct cgtccgccgg gatctcgacc gtgatcttcc
     1021 gttcgacgcc ggaaacggcg tccacgctgg ttttcatcgc tctacggttc cccttttcca
     1081 ttcgatgatg ttcgacggca acctggtgcg agaggaggga cttgaaccct cacggttgcc
     1141 caccggatcc taaatccggc gcgtctgcca ttccgccact ctcgccccgt tcgacggcgc
     1201 cgaattatac agtatagacg attcctccgc ccgcctcaac gccggacaat ccgccgacgg
     1261 actttatccg gggtttttcc gggcggcgag ccggagttcc tcgagggacg gcgcctcctc
     1321 gtcgaaccgc atgccgaaga tgtagaatcc cggaggcgcg tccgccgcgc ggaaccagat
     1381 caccgtcccc tgcacccgga caggcggttt cccttccgga aaaatggtgg cgtcgatccg
     1441 gttccggaag gtcatgagcg tgtcgtacat gatgtggatg ccgtccgggg cgagccgggg
     1501 agtgaccacg gacatccccg tggaggagac gtcctgggcg tccccggtga cgggggaaga
     1561 aacccgcccc ggatcctttc cgctgcgcac tgtaaaaacg acggtggcca atggacgata
     1621 ccgctccttc gctcttcgtt cccggcggaa gaaggtcatc cggtcgggtc ttccttcccc
     1681 tcctcgtcga ggcggcgcag cccttccatc atgagctgca tctcgtccat ctcgatcgtg
     1741 cggagtttcg tcgcctgccc gtgcgcgatg acgaacggcc cctcctgcca gcgaagcatc
     1801 tcgtagaagg ccagctcccc cgagaggctc cccagctcgg catgccggat ccgtccgttc
     1861 tcgaaccaga tcttcccttc ggcaccattc ccggtgacgc ggacgcaggc ggtcttcaga
     1921 cccaggtgaa gggtctggac gatgtcgggg acgccgaggt ttctgaggtc ccccatcacc
     1981 cccgcggact gggcgatccc cgacgccccc ttgcgccact tcgcgtcgcg ccggatgatg
     2041 tttcggattc gcgcggccag ctccttcagg tcgaccggct tgatgaggaa atcctcggcg
     2101 ccaaggtgca gcgcgtcgac cttggtctgc gaatcgttct tgccgctgac gaagatgagg
     2161 ggggtgtctc cccagggggt gttcgcctcc cgcatcttgc ggaacatcgc gatcccgtcc
     2221 atccccggca tcatcacgtc ggccacgacc aggtcaggcg ggtccttctc catcgcggcc
     2281 agcgcgtccc ccgcgttgtc cgcggcggcg acgtcgaacc cctcgttcac gagtttcagc
     2341 ttgaggagca tgaggtagtc gatctcgtcg tccaccatca gtatcctcgg cttcggcccg
     2401 agcccgcgga aaaggaactt ccggcccacg acggaggtga acagctccac cacctcgggg
     2461 tcgaactggg tcccgatgtt cttgtggagc tcctggatcg cctcctcgcg cgtgaggctc
     2521 cggctacggt acgggcggtg ggcggtcatc gcggtgaagg cgtcgaccac ggccaggatc
     2581 cgggcgccga tcgggatctc ccgccccttc aggccgctcg gatacccttt cccgtcgtac
     2641 cgctcgtggt ggtggatgat gatcggcttg atcttccacg ggaagtcgac cgcgtcgatg
     2701 atcttcgccc cgttttcgca gtgggaccgt accgtcttga actccgactc cgagatctcc
     2761 cgcttgccga gcaggatgtc gctcttgatg ccgacccggc cgatgtcgtg gaggagcgat
     2821 ccgacaacga tctcgtccac cgtttcccgg tcgagcttca tctcctcggc cacgaaacgg
     2881 gcgtactcca tcgtgatgtg ggagttcccc ccgaagaacg ggtcgttcac ctccagcagg
     2941 ctgaccagga tgtcgaccgt gtcgatcagg catttcctga gctgctcgtc ctcggacacc
     3001 ggcttcgcga tcaccggctg gaccgcggag gtcgcacgaa ggagctcgac gatttccttc
     3061 tggtcgagga tgaagtcgga cgtgccgaac cgaaggatgt cgtcggagtt gcggatcgtt
     3121 gtgaaggagg acaggacgac gacgcgggtc ttcggggaga cgcgggcgag acgccgccgg
     3181 aactcgacgc cggaggaatc gggaaggaac agttcgacga ggacggtgtc gaagaacgcg
     3241 ttcgcggcga tcttcagggc ctccgtgccg gatcccgcga gcgcgacgtg gaacccctcc
     3301 tcggtgagga gattcgccag catgtcccgc acaaccgggt cgttgttgac gaccaggacc
     3361 ggggttcgcg tttcccgcga cggttccacg tcagacctcc cggacatccg tgccggtcat
     3421 ttccgccgga acgggcagcg tcagaaggtc gagaatcgtc ggggcgaggt cctcgagcgc
     3481 gcggtcttcc cggagccggg tgccgacggc ccgcgggtcg gcgagaacga gcggcacgag
     3541 gttcgtcgtg tgcgcggtga agggaccgcc ggtcttcggg tcgatcatct gctcggcgtt
     3601 gccgtggtcg gcggtcacga gcgccacccc tccgacttcc cacactttct ccacgacccg
     3661 ctgcaggttc cggtccaccg cttcgatcgc ctggatcgcc gcagggagaa cgcccgtgtg
     3721 ccccaccata tccccgttgg cgaagttgag gaccatcagg gcgtgctttc ccgaggcgat
     3781 ctcggccacg gcgcgctccc caacctcgta cgcgctcatc tcgggcttga ggtcgtaggt
     3841 ggacacggac ggcgacggga tcaggacccg cgactccccg gggtagaccg tctcctcccc
     3901 gccgttgaag aagtacgtga cgtgggcgta cttttccgtt tcggcgatcc ggaggtttgt
     3961 aagacccgcg gcggccagcg accgcgcgag gatgttgtcg agccgctgcg tggggaaggc
     4021 cgcgggaagc ccgaacgtct cgtcgtaggg ggtcatgcag gcgtaggcga gagaaagccg
     4081 ctcggggcgt gggaagcggt cgaacgtctc ctgcgtcagc gcccgggtga tctcgcgcgc
     4141 acggtcggcc cggaagttga agaagatcac cgagtcgccg gaggcgatcc gtccgaccgg
     4201 ccggccgtcc cgtacgatca ccacgggctc gatgaactcg tcggtcttcc ccgccgcgta
     4261 cgacgcggcg acggcggcca cggggtcgcc ggaggtggtc ccctccccgc ggaccatcgc
     4321 cttgtacgcg cgctcgacgc ggtcccaccg gttgtcgcgg tccatcgtgt agtaccgtcc
     4381 gacgacggtc gccacctcac cggcgccgat ctcggccgcc ttcgccaaca aggcgcggag
     4441 gtggtcgatc ccgctttgcg gcggggtgtc ccgcccatcg agcacgggat ggaggcacac
     4501 cttcgggacg ccgcgctcct tcgccatccg cagcagcgcg tagaggtgtg tgtgaaggga
     4561 gtgcacgccg ccgtcggaga gcaggcccac gaggtgcagc gccttcccgt cttccctcgc
     4621 cgcgtccatg gcggcgcaaa ggacggggtt ccggaagaag tcgccggtcc ggacggactt
     4681 cgagatgcgg acgaggtcct ggtacaccac gcgtcccgtc cccaggttaa ggtgcccgac
     4741 ctcggagttc cccatctgac cggccggaag ccccacgcgc tcctccgagg cgtggatcag
     4801 ggttcgcggg aaccccgccc agatccggtc gaagaaaggc gtgtcggcaa gggcgaccgc
     4861 gttcccttcc ttctcttcgc gatggcccca accgtccatg atgatcagcg cgacgaatgg
     4921 gcctttcatt tcagatatta tagaacactc cccgcccgtc gaaccgggcc gccgggccga
     4981 gttcctcctc gatccggagc aactggttgt acttcgcgat ccggtcggtc cgcgaggctg
     5041 agccggtctt gatctgtccg gtcgagagtc ccacgacgag atcggcgatc gtactgtcct
     5101 cggtttcgcc ggatcgatgg gacacgacgg cggtccaccc cgcgcgcttc gccatctcga
     5161 tcgcctcgat cgtctccgtc acggtgccga tctggttcag cttgatgagg acggagttgg
     5221 ccaccccctg cgcgatcccc ttccgcaaaa tcgagggatt ggtgacgaag atgtcgtccc
     5281 ccacgatctg gatcttcttt cccatcgcat ccgtgaacat cttccagcct tcccagtcgt
     5341 cctcggagaa gccgtcctcg atcgagacga tcgggtactg gcggcacagc tcctcgtaga
     5401 acctgacgag ctgcgcggag tcccgcttcg acttgtcgga tttgcggaat acgtatttcc
     5461 ccttctcgcg gaactcggag gcggcggagt cgagggcgac gcagatgtcc ctcccgggct
     5521 tgtacccggc cttcgtgatc gcctcgagga tcacctcgat cgcctcggcg ttggaccgca
     5581 gctggggggc gaacccaccc tcgtctccga cgttcgtgtt caaccccttc cccttcaaca
     5641 ccttcttcag attgtggaac gtctcgaccc ccatccggag cgcctcggag aagctcttcg
     5701 cccccaccgg catcaccatg aactcctgga tgtccatgtt gttgtcggcg tgggagccgc
     5761 cgttcaggat gttcatcatc gggacgggga gggtgcaccc tccgactccg ccaaggtacc
     5821 ggtacaacgg gaggccgcac gcctccgccg ccgcccgtgc caccgcgatc gaggcgccga
     5881 ggatcgcgtt cgcccccagc ttccccttgt tctcggtgcc gtccaggtcg atcagcatcc
     5941 ggtcgaccag gacctgttcc gtcgcgtcgt atccgatcag tttgggggcg atcacccggt
     6001 tcacgttgcg gaccgccttc gtcacgccct tccccatgaa ccgcttcggg tcgccgtcgc
     6061 gcagctccac ggcctcgcgg gtcccggtcg acgccccgga ggggacgatc gccgcccctt
     6121 ccgcgccgga ctcgagcagc acctcgacct cgacggtcgg gttcccccgg gagtccagaa
     6181 cctcccttcc gtgcacatcg acgatcatcg tcatgtcgtg ttccccctac gtgcgaagtg
     6241 atccgaatta cggatgaacg aattccttgc cgccttcgcg gaccgaaagc acggggcatg
     6301 gcgccatgcg gacgaccttc tcggcggtgc tgccgaagat cacgcgttcc accccggtgc
     6361 ggccgtgcgt cccgatgacg atcagcccgg ccccgcactc ccgggcttcg cggacgatct
     6421 cccggtaggg aacgccggag gccgtccggg tctccactcc cgggagcccg tcgaagtggg
     6481 ccttgaggaa cgcttcgatc ccctcgcggg cgtactgctc catccgcccc cggagcaact
     6541 cgagcgggac ctcgccccgg aacatcgggt cgagcgccgc gggttcgtcg aggacgtgga
     6601 ggacgatcag gcgggatccg aagcactcgg ccaaccggcg cgccgcgcgg gccgcctcgg
     6661 cggaacatcc ggaaaaatcg gaaggcaaca ggatcgtcgt gaacatggcc acccccggaa
     6721 tggaaacgcc ccggccggat accggttcgg ccggggcgca aaatctatcg gagaggcgac
     6781 tgccggaacg gacgca
//
LOCUS       DASVJL010000011         4531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_845863, whole genome shotgun sequence.
ACCESSION   DASVJL010000011 DASVJL010000000
VERSION     DASVJL010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845863"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /gene="nuoB"
                     /locus_tag="VF357_00200"
     CDS             <1..418
                     /gene="nuoB"
                     /locus_tag="VF357_00200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX7522592.1"
                     /translation="SLYPFTFATACCGIEVMGAFGTHYDLSRFGAEVIRFSPRQADVL
                     LVAGTINYKMAPVLKRIYDQMLEPKWVISMGACACSGGFYNNYTVLQGIDKIMPVDVY
                     IPGCPPNPEGIIDAVVQIQKIIETGAPRAAERWPIK"
     gene            697..1173
                     /locus_tag="VF357_00205"
     CDS             697..1173
                     /locus_tag="VF357_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX7522593.1"
                     /translation="MSVVLDRLRARFPADVVSTHSDFGDDTALIRRERIVEILTFLRD
                     DPEMLFDFAMDLTGVDYLGEEPRFEVVYHLYSLEKKRRVRIKVRLHEDDPVIDTAVSV
                     WPGINWYEREAWDMYGIVFHGHPNLKRILLYDAFEGHPLRKDYPKAKRQPTIGPEE"
     gene            1342..2559
                     /gene="nuoD"
                     /locus_tag="VF357_00210"
     CDS             1342..2559
                     /gene="nuoD"
                     /locus_tag="VF357_00210"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX7522594.1"
                     /translation="MAEPVDITTKGGTPDLQAEPMLINIGPSHPATHATLQFRAKLDG
                     ETILDLEPEFGYLHRGFEKESEASTWTQVVPYTDRLNYVSPLMNNVGYAMAVEKLCGI
                     EITERCKYVRVIISELSRIIDHMVCIGTNMVDLGALTNFWYFWKPREEVYFLIEALCG
                     QRLTTSYTRIGGAMADLPEGWTDRALAVCRGVIPECIADVDALLTKNRIFIERTMGAG
                     PITAKDAIALGFTGPCLRAAGVPLDLRKDEPYLVYDRFDFDVPVGEAGDTCDRYMVRM
                     EEMRQSLRIIEQAIAKLPGGPINIDDPRFILPGKAQVYENIEALMNHFKFIMEGVKVP
                     AGEVYSATEAANGELGFYIVSRGGGGPYKIKVRPPCFPLFQGIPHLVKGQMIADLIAV
                     LGSVNIIAGELDR"
     gene            2556..3029
                     /gene="nuoE"
                     /locus_tag="VF357_00215"
     CDS             2556..3029
                     /gene="nuoE"
                     /locus_tag="VF357_00215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX7522595.1"
                     /translation="MSIVFSETGCAEFERLLTRYPDRKAVILPALHLAQKEFGYVSDE
                     AIAYIAGLVGTSSSEIEGVATFYTMYNRKPVGKYHVQICRNISCSLLGAEHLIEHVSK
                     KLGVRPGETTPDGKFTLTKVECLGSCGTAPVMQVNDEYHENLTVESIDRILDQLP"
     gene            3049..4317
                     /gene="nuoF"
                     /locus_tag="VF357_00220"
     CDS             3049..4317
                     /gene="nuoF"
                     /locus_tag="VF357_00220"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX7522596.1"
                     /translation="MEKVLTTHFANERYRRLDTYRQYGGYEALKKALAMDPGQIIDEV
                     KKANLRGRGGAGFPAGVKWGFLPKDLSRPRVLVINADEGEPGTFKDRVVMTRGPHLLI
                     EGIVISCFALRAHACWIYIRGEFVREARIMDEAVAEAYAAGFLGKNILGSGFDLDLTV
                     HRGAGAYICGEETSLINSLEGKRGWPRLKPPFPASVGAFGLPTIVNNVETIADIPWII
                     THGGETFAALGVEKNGGTRLIGVSGAVNRPGVYELSAGVNLKDIIYHHAGGIKDGKAL
                     KAVIPGGSSTPVLRPNEIDVSYDIESMAKIGTMAGSGGVIVIPEGACMVRALSVLMNF
                     YAHESCGQCTPCREGTGWLKKLVGRIEAGEGRQGDVELILDLCDNMMGRTICPLADAA
                     VMPAQSFIWKFREEFDRHIGEQKCPYGNRF"
     gene            4327..>4531
                     /locus_tag="VF357_00225"
     CDS             4327..>4531
                     /locus_tag="VF357_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7522597.1"
                     /translation="MPTLTINGMTVDVPVGTTIINAAEKIGVEIPRYCYHPKLSIAGN
                     CRMCLVEVEKFPKLQTACSTVVTD"
BASE COUNT          860 a         1402 c         1560 g          709 t
ORIGIN      
        1 ctccctctac ccgttcacct tcgccacggc gtgctgcggc atcgaggtga tgggggcgtt
       61 cgggacgcac tacgacctgt cccgcttcgg tgccgaggtc atccgtttct cgccgcgcca
      121 ggcggacgtc ctcctggtgg cggggacgat caactacaag atggcccccg tgctgaagcg
      181 gatctacgac cagatgctcg agccgaagtg ggtgatctcg atgggggcgt gcgcctgctc
      241 cggcggcttc tacaacaact acacggtcct ccaggggatc gacaagatca tgcccgtcga
      301 cgtctacatc cccgggtgcc cgccgaaccc cgaggggatc atcgacgccg ttgttcagat
      361 ccagaagatc atcgagaccg gcgcgccccg cgccgccgaa cgatggccga taaagtaagt
      421 tggatagcag atacgttcat gcatcgagac gagataaggt agcattgctc cgttcatgat
      481 gcctatccgg cggggtacgg acgaagggga ccttcccctg agcgggcgtc tgaagcgagt
      541 gtagccaggg acggcgggaa ggagcggaag cgcagcgcgg gcggccaggg atggccgccg
      601 agcggtagcg aaggggaatg gcccccgagg aagaggggtc cccgcacaaa ggcgggtcga
      661 acggacggac caacgggcaa ggagaggaaa agcggcgtga gcgtggtcct cgacagattg
      721 cgggcgcggt tcccggccga cgtggtgtcg acccactcgg acttcggtga cgacacggcg
      781 ttgatccggc gggagcggat cgtcgagatc ctcacgtttc tgcgggacga cccggagatg
      841 ctgttcgact tcgcgatgga tcttaccggg gtcgactacc tcggggagga gccccggttc
      901 gaggtggtct accacctcta ctcgctggag aagaagcgcc gcgtccggat caaggtgcgc
      961 ctccacgagg acgacccggt gatcgacacg gccgtttcgg tgtggcccgg gatcaactgg
     1021 tacgagcggg aggcgtggga catgtacggc atcgtcttcc acggccaccc gaacctgaag
     1081 aggatcctgc tgtacgatgc gttcgagggg cacccgctgc gcaaggatta cccgaaggcg
     1141 aagcgccagc ccacgatcgg gccggaagag tgagggtcgg tccaggaata agatgttcgg
     1201 atatgggcgt tgagtcgccc ggccggcaag gcacgaggag ggagaatacc gtgagtattc
     1261 gacagacgag caacgcagcc cgccgggatg aatcggcgtc cagatcgcac aggttattcc
     1321 tggacaggcc ctgagggagc gatggcggaa ccggtcgaca tcactacgaa gggcgggacg
     1381 cccgacctgc aggccgagcc gatgctcatc aacatcgggc cgtcccaccc cgcgacgcac
     1441 gcgacgctgc agttccgggc gaagctcgac ggggagacga tcctcgacct ggaaccggag
     1501 ttcggatacc tccaccgcgg cttcgagaag gagtccgagg cctccacctg gacccaggtg
     1561 gtcccgtaca ccgaccggct gaactacgtc tcgccactca tgaacaacgt agggtacgcg
     1621 atggcggtgg agaagctgtg cggcatcgaa atcacggagc ggtgcaagta cgtccgcgtc
     1681 atcatctcgg agctctcccg gatcatcgac cacatggtgt gcatcgggac gaacatggtc
     1741 gacctcggcg cgctgacgaa cttctggtat ttctggaaac cgcgcgagga ggtctacttc
     1801 ctgatcgagg cgctgtgcgg gcagcggctg accacttcgt acacccggat cggcggggcc
     1861 atggcggacc tgcccgaggg gtggaccgac cgggccctcg cggtatgccg cggcgtcatc
     1921 cccgagtgca tcgccgacgt cgacgcgctc ctcacgaaga accggatctt catcgagcgg
     1981 acgatgggcg ccgggccgat cacggcgaag gacgcgatcg ccctgggctt caccggaccg
     2041 tgcctgcggg cggcgggggt ccccctcgac ctgcgaaagg acgagccgta cctcgtgtac
     2101 gaccggttcg acttcgacgt gcccgtaggc gaggcgggcg acacgtgcga ccggtacatg
     2161 gtgcgcatgg aggagatgcg gcagtcgctc cggatcatcg agcaggccat agcaaagctc
     2221 cccggcggcc ccatcaacat cgacgacccc cggttcatcc ttccggggaa ggcgcaggtg
     2281 tacgagaaca tcgaggcgct gatgaatcac ttcaagttca tcatggaggg ggtcaaggtc
     2341 ccggccggcg aagtctactc ggcgaccgag gcggccaacg gcgagctcgg gttctacatc
     2401 gtgagcaggg gcgggggcgg gccatacaag atcaaggtcc ggccgccgtg ctttccgctg
     2461 ttccagggga ttccgcacct cgtgaagggg cagatgatcg cggatctcat cgccgttctg
     2521 ggaagcgtca acatcatcgc gggggagctg gaccggtgag catcgtcttt tccgaaaccg
     2581 ggtgcgcgga gttcgagcgc ctgttgaccc gctaccccga caggaaggcg gtgatcctgc
     2641 ccgcccttca cctggcgcag aaggagttcg ggtacgtctc cgacgaggcg atcgcctaca
     2701 tcgccggact ggtgggcacc tcttcctcgg agatcgaggg agtcgccacc ttctacacga
     2761 tgtacaaccg gaagcccgtg gggaaatacc acgtgcagat ctgccggaac atttcctgct
     2821 ccctcctcgg ggcggagcac ctgatcgaac acgtgtcgaa gaagctcggc gtccggcccg
     2881 gggagacgac cccggacggc aagttcaccc tgacgaaggt ggagtgcctg gggtcctgcg
     2941 gcacggcgcc ggtaatgcag gtcaatgacg agtaccacga gaacctgacc gtcgaatcga
     3001 tcgaccggat cctcgaccaa ctcccatgag gagctgacgc aggcggccat ggaaaaggtc
     3061 ctcaccacgc atttcgccaa cgagcggtac cgccggctgg acacgtaccg gcagtatggc
     3121 ggctacgagg cgttgaaaaa agccttggcg atggaccccg gccagatcat cgacgaggtg
     3181 aagaaggcga acctacgcgg ccgcggcggc gccgggttcc cggcgggggt caagtgggga
     3241 ttcctcccga aagatctctc ccgcccgcgc gtgctggtca tcaacgcgga cgagggggag
     3301 ccggggacct tcaaggaccg ggtcgtcatg acccgcgggc cgcacctcct gatcgagggg
     3361 atcgtcatct cctgcttcgc cctgcgggcc cacgcctgct ggatctacat ccggggcgag
     3421 ttcgtccgcg aggcgaggat catggacgag gcggtcgccg aggcgtacgc ggcggggttc
     3481 ctcgggaaga acatcctggg gtcggggttc gacctcgacc tgacggtcca ccgcggcgcg
     3541 ggcgcctaca tctgcggcga agagacttcc ctgatcaact ccctcgaggg gaagcgcggg
     3601 tggccgaggc tcaagccccc cttcccggcg tcggtggggg ccttcgggct gccgacgatc
     3661 gtgaacaacg tggagaccat cgcggacatc ccctggatca tcacccacgg aggggagacg
     3721 ttcgccgccc tcggcgtgga gaagaacggc gggacgcgcc tgatcggcgt cagcggcgcc
     3781 gtcaaccggc ccggcgtcta cgagctttcg gcgggggtca atctgaagga catcatctac
     3841 caccacgccg ggggcatcaa ggacgggaag gcgctcaagg ccgtcatccc cggcgggtcg
     3901 tccacgcccg tgctgcggcc caacgagatc gacgtgtcct acgacatcga gtcgatggcg
     3961 aagatcggga cgatggccgg gtccggcgga gtgatcgtca tcccggaagg ggcctgcatg
     4021 gtgcgggccc tctcggtcct catgaacttc tacgcccacg agtcgtgcgg gcagtgcact
     4081 ccgtgccgcg aggggacggg gtggctcaag aagctcgtgg ggaggatcga ggcgggagag
     4141 gggcggcagg gcgacgtcga gctgatcctc gacctgtgcg acaacatgat ggggcgcacc
     4201 atctgcccgc tggcggacgc cgcggtgatg ccggcgcaat cgttcatctg gaagttccgc
     4261 gaagagttcg accgccacat cggcgagcag aagtgcccgt acgggaaccg gttctgaaac
     4321 gagacgatgc cgaccctgac catcaatgga atgaccgtgg acgtccccgt cgggacgacg
     4381 atcatcaatg ccgcggagaa gatcggcgtc gagatccccc gctattgtta ccacccgaag
     4441 ctttcgatcg ccgggaactg ccggatgtgc ctcgtggagg tcgagaagtt cccgaagctg
     4501 cagaccgcct gctccacggt ggtcaccgac g
//
LOCUS       DASVJL010000012         3025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_845879, whole genome shotgun sequence.
ACCESSION   DASVJL010000012 DASVJL010000000
VERSION     DASVJL010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845879"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="VF357_00230"
     CDS             complement(<1..1367)
                     /locus_tag="VF357_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX7522598.1"
                     /translation="MEKARADADTVLKELGSQPGGLSEAEADSRLKQVGTNEIAREKR
                     QSALMRLLSNIKNPLVLLLLALGVLSYLTGDLRAMVVIFVMVVLGVVLRFFQETRADN
                     AAEKLKAMVSNTATSVRDGKEAEVSLKMLVPGDIIRLAAGDMVPADVRVLSAKDLFLN
                     QAALTGESLPVEKKAAPALADVQNPLDLPNLCFLGSNVESGSATAVVIHTGDRTYLGS
                     LAASIVGQRELTSFDKGVNKFTWLMIYFIAVMVPAVFLINGLSKHNWLEAFLFAMAVA
                     VGLTPEMLPMIVTVNLSKGAIAMARKKVIVKRLNAIQNFGAMDVLCADKTGTLTQGKI
                     VLEKHLDVHGDPSEEVLHYGYLNSYHHTGLKNLLDEAILDHEELEERLKAKEKYRKID
                     EIPFDFVRRRMSVVVEDETGLNTLICKGAVDEVMRRCTRVNLHAELVAVEPEHDTKRR
                     QIADDL"
     gene            1628..>3025
                     /locus_tag="VF357_00235"
     CDS             1628..>3025
                     /locus_tag="VF357_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7522599.1"
                     /translation="MKVAIIGGVAGGASAAARARRLDETAEIVLFERGEYISFANCGL
                     PYHVGKAIPRRDSLLVMTPKRFNARTGVDVRIRQEVTAIDPAAHTLSILDRATGNTYT
                     ESYDKLILSPGAGPIRPDVPGADDPAVMMLWTLPDMDRIKARVDAGIRRAVVVGGGFL
                     GLELAENLRLRKVEVTLVEMLPQVMPPLDPEMAQPLIAELERNGVKVLLGSTVIGVRR
                     LAERDGSGSPEVVVGLGDGRELPADLVVFTIGVRPNSSLAKSAGLATGERGGIVVNTR
                     LQTSHPDIYAIGDAIQITDALGLSSQIPLAGPANRQGRISADNVFGAEREYKTTFGTS
                     VVKVFDMTAACTGATEKALRKAGVPHEKVYLHPYSHATYYPGARMMHLKLLFTQEGRI
                     LGVQIVGHDGVDKRIDVLATAMQAGLTVYDLQELELAYAPPYGSAKDPVNFAGFVASN
                     LLRGETQVVHADALPV"
BASE COUNT          556 a          942 c          919 g          608 t
ORIGIN      
        1 aggtcgtccg ctatctgcct tcgtttggtg tcgtgctcgg gctcgacagc aaccagctct
       61 gcatggaggt tcacccgagt gcaccggcgc atcacttcat ccaccgcgcc cttgcagatc
      121 agcgtgttca acccggtttc atcttccact accaccgaca tccgtcgccg cacaaagtcg
      181 aaggggatct cgtcgatctt gcggtacttt tctttcgctt tcaggcgttc ttccagctcc
      241 tcgtggtcga ggatcgcttc gtcgagcaga ttcttcagcc cggtatggtg gtagctgttc
      301 aggtagccat agtgcagcac ctcctcgctg ggatcgccgt gcacgtccag gtgcttttcg
      361 agcacgatct ttccctgggt cagggtgccg gtcttgtcgg cgcacagcac gtccatcgcc
      421 ccgaagttct ggatggcgtt caggcgtttg acgatcactt tcttgcgcgc catcgcgatg
      481 gcgcctttgg acaggttgac ggtcacgatc atgggcagca tctcgggtgt cagcccgacg
      541 gccaccgcca tggcgaacag gaaggcttcc agccagttgt gcttgctcag cccgttgatc
      601 aggaagaccg ccggaaccat cactgcgatg aagtagatca tcagccaagt gaacttgttg
      661 acgcccttgt cgaagctggt caactcgcgc tgcccgacga tactggcggc cagcgatcca
      721 aggtaggtcc ggtccccggt atggatgacc accgccgtcg cggagccgct ctccacgttg
      781 gatcccagga agcagaggtt gggcagatcg agaggatttt gaacgtctgc caacgccggg
      841 gcggctttct tctccaccgg cagggattcg ccggtgaggg ccgcctggtt caggaacagg
      901 tctttggcgg aaagcacccg tacatcggct ggcaccatgt caccggctgc cagccggatg
      961 atatcgccgg gcactaacat cttgagcgat acttctgctt ccttgccgtc tcgcaccgac
     1021 gtcgctgtgt tgctgaccat cgccttgagc ttctcggcag cgttatcggc acgcgtctcc
     1081 tggaagaaac gcaacactac gcccaaaacc accatcacga aaatgaccac catcgctctc
     1141 agatccccgg tcaggtagga aagcacaccc agcgccagga gcaggaggac caatggattc
     1201 ttgatgttgc tcaggaggcg catcagggcc gattgccgct tttcgcgggc gatctcgttc
     1261 gtcccgacct gcttcaagcg agaatcggct tccgcctcgc tcagcccgcc tggttgcgat
     1321 ccaagctcct tcaggacggt atcggcatcg gcacgcgcct tttccaatag ttcaacggaa
     1381 gaatgccccc cgtttccacc agggaggttg cctgggcgct gagatgcgtt cagggtcttc
     1441 tttggaatat ccattcacac ctctgctgtg tcctgtatcg tcttgacctt gtcgagatca
     1501 tcgtcaggat ccgttgaagt atactatggg ccgtttccag ccccggcgcg gatcggcaat
     1561 tccgaccttc cggcgtgttc gaatgcctgc aacgccaccg gggaatcacg gtgaaaaggg
     1621 gtacatcatg aaagttgcca tcatcggcgg agtggcgggg ggagccagcg cggcggcaag
     1681 agcgcgtcgg ctggacgaaa ccgcggagat cgtcctgttc gagcggggcg agtacatatc
     1741 gtttgccaac tgcggcctgc cgtatcacgt ggggaaggcc attccaaggc gcgacagcct
     1801 gctggttatg actccaaagc ggttcaatgc ccgcaccggc gtggatgtgc gcatacgcca
     1861 ggaggtcacg gccatagatc ccgcagccca cacgctctcc atcctggacc gggcgaccgg
     1921 caatacgtac acggaaagct acgacaagct gatcctctcc cctggcgcag gcccgatccg
     1981 gcccgacgtc ccgggcgcgg acgacccggc ggtcatgatg ttatggacgc tgccggacat
     2041 ggaccggatc aaggcgcggg tggatgccgg cattcggcgc gccgtcgtgg tggggggcgg
     2101 gtttctgggc ctggagttgg ccgagaacct tcgcctgcgc aaggtcgaag tgaccctggt
     2161 ggagatgctg ccgcaggtca tgcctccact ggaccccgag atggcgcaac ctttgatcgc
     2221 ggagcttgag cgaaacggcg tcaaggtgct gctcgggagc acggtgatcg gcgtccgccg
     2281 cctcgcggaa cgggacggtt ccggatctcc ggaggtcgta gtcggacttg gcgacgggag
     2341 ggaactcccc gcggatctcg tcgtttttac catcggcgtc cggccgaact cgtccctggc
     2401 gaaatcggcc ggccttgcga ccggagagcg gggaggaatc gtcgtcaaca ctcgcctgca
     2461 gacaagccac ccggacattt acgcgatcgg cgacgccatc cagattacgg acgctctcgg
     2521 cctttcgtcg caaatccctc ttgccggccc ggcgaaccgt caggggcgaa tctcggccga
     2581 caatgtcttc ggcgcggagc gtgagtataa aaccacgttc ggcacctctg tggtcaaggt
     2641 gttcgatatg acggcagcct gcaccggcgc tacggaaaag gctctccgga aggcgggcgt
     2701 gccgcacgaa aaggtatacc tccatccata ttcgcacgcc acctattatc cgggcgcgcg
     2761 gatgatgcat ctcaagctgc tattcacgca ggaagggcgg atcctcggcg tccagatcgt
     2821 cggacatgac ggcgtggaca agcggatcga cgtgctcgcc accgcaatgc aggccgggtt
     2881 gaccgtgtac gaccttcagg aattggaact tgcctacgct cccccgtacg gttccgccaa
     2941 ggacccggtc aacttcgccg gattcgtggc gtccaacctt ctgcgcggcg aaacacaggt
     3001 ggtccatgcg gacgctcttc cagtg
//
LOCUS       DASVJL010000013         4083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1408873, whole genome shotgun sequence.
ACCESSION   DASVJL010000013 DASVJL010000000
VERSION     DASVJL010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408873"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VF357_00240"
     CDS             complement(<1..945)
                     /locus_tag="VF357_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522600.1"
                     /translation="MGTHPNAGKPAEPKDLVNVPRLVAAYYTGNPDPSEPSQRVAFGT
                     SGHRGSSLLLSFNEAHILAVTQAICEHRKREGATGPLFLGMDTHALSEPAFRSALEVL
                     GGNGVEARVDRDGGYTPTPVISHAILSHNRGRREGLADGIVITPSHNPPEDGGFKYNP
                     PHGGPADTGDTREIEERANGILRSGPGEVRRLPFERALAAGTTRRHDYVGAYVGDLGA
                     VLDLEAIRGAGIRIGVDPLGGSGAAYWEPIADRYGLNLAVVNPAVDPTFRFMPLDWDG
                     KIRMDCSSPFAMAGLIAMRDRFDVAFGNDTDADRHGIVT"
     gene            complement(974..1720)
                     /gene="wrbA"
                     /locus_tag="VF357_00245"
     CDS             complement(974..1720)
                     /gene="wrbA"
                     /locus_tag="VF357_00245"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX7522601.1"
                     /translation="MPRELVDNLLTSLFLAVNLPGERGILKVEQFGIPVPNRTDTEGE
                     AMKALIVFYSMYGHVYRMAEAVAEGAREVPGAEVTLRRVPETLAAAVLQKMGAIEAQK
                     AFAHVPVCSVEELPSYDAIFFGTPTRFGNMCGQMRQFLDATGQLWSAGALVGKIGSVF
                     ASTATQHGGQESTLLSTHITLLHQGMVVVGLPYAFQGQMRIDEITGGSPYGATTIAGT
                     KGERSPSENELAAARFQGKHVASIAAKLVR"
     gene            1767..>4083
                     /locus_tag="VF357_00250"
     CDS             1767..>4083
                     /locus_tag="VF357_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEX7522602.1"
                     /translation="MTAKSNPFRINASGVPKPTGLYDPQNDHDSCGVGFIARIDGVSL
                     HTVVEQGIRILVNLEHRGALGGDKSTGDGAGLLLEVPDTFFRQVCPGGGLYLPPRGEY
                     AVGMLFLPMDEALAGRCSDTLERIAAAEGCPVLGWREVPVDPSILGDLSGSTRPRIRQ
                     LFLARGPHERDAFERKLYVIRRLTEKKVASWHDVDASQFYISSLSSRTIVYKGLLTGS
                     QLPLFYPDLKNELFMSPYAVVHQRYSTNTLPTWHLAHPFRIAAHNGEINTLRGNINRM
                     RAREANLASPLFGNDIAKLRPVINEAGSDSAIFDNVLELLVMSGRSLPHAIMMMIPEA
                     WGAKFQMSEDKRSFYEYHSAIMEPWDGPAAMVFCDGRYLGATLDRNGLRPARYTVTRD
                     GMIVLASETGVMDIPPDRIVRRGRLQPGKMLLLDLQQKRIVPDNEIKAAISRRKPYRK
                     WVKDNKIELRGMFKPSEIPPEDPETLRRKQHAFGYTEEEIKLIITPMASQGQEAIGSM
                     GDDAALAVLSNRPQSLFAYFKQLFAQVTNPPIDPLREELVMSLNGFIGRERNLLDETP
                     EHCRMLRLIQPILTPEDMLRLRGSTHPDLAAADLDMLFPAGGDGKALETALARLFVQG
                     DKAIRGGATVLVLTDRNMNAGRAPIPSLLATAGLHHHLIRRGLRTQASIVVESGEPRE
                     VIHFALLLGYGANAICPHTVLSTIRELAENEGLEAPLLAVEAVDKYVTSVKKGLLKTF
                     SRMGISTLRSFLGSQIFEAVGLGKELVDGYFT"
BASE COUNT          681 a         1431 c         1307 g          664 t
ORIGIN      
        1 cgtcacgatg ccgtgccggt cggcgtccgt gtcgtttccg aaggcgacgt cgaaccggtc
       61 gcgcatcgcg atcagcccgg ccatcgcgaa cggggaggag cagtccatcc ggatcttccc
      121 gtcccaatcg agcggcatga accggaacgt cggatcgacg gcggggttca ccaccgcgag
      181 attcaagccg taccgatcgg cgatcggttc ccagtacgcc gccccggagc cgcccagagg
      241 gtcgacgccg atccggatgc cggccccgcg gatcgcctcg aggtccagca ccgcgccgag
      301 gtcgccgaca taggcgccga cgtagtcgtg acggcgcgtc gtacccgccg caagggcccg
      361 ctcgaagggg aggcgccgca cctccccggg ccccgaccgc aggatcccgt tggcgcgctc
      421 ctcgatctcc cgggtgtccc ccgtgtccgc cggcccgccg tgcggcgggt tgtacttgaa
      481 tcccccgtct tccggcggat tgtgggacgg ggtgatcacg atcccgtcgg ccagaccctc
      541 cctccgtccc cggttgtgcg agaggatggc gtgggagatc accggcgtcg gcgtgtagcc
      601 tccgtcccgg tccaccctcg cctcgacccc gttccccccc agcacctcga gcgcgctgcg
      661 gaacgccggt tcggaaaggg cgtgggtgtc catcccgaga aagaggggcc ccgtggcccc
      721 ctccctcttc cggtgctcgc agatcgcctg cgtcacggcg aggatgtggg cctcgttgaa
      781 agaaaggagg agggacgagc cgcgatggcc ggacgttccg aacgccaccc gctgcgaagg
      841 ttcggacggg tcgggatttc cggtgtagta ggccgcgacc aaccgcggga cgttgacgag
      901 gtccttcggt tcggccggtt tccctgcgtt cgggtgtgtc cccacgcctc actctccttg
      961 ggtgcgctcg attttaacga accagtttgg cggcgatgga cgccacgtgt ttcccctgga
     1021 accgcgccgc cgccagttcg ttctccgagg gcgaccgctc gcctttcgtg ccggcgatcg
     1081 tggtggcgcc gtacggggag ccgccggtga tctcgtcgat ccgcatctgc ccctggaaag
     1141 cgtacggcag tccgacgacc accatcccct ggtgcaggag ggtgatgtgg gtgctgagga
     1201 gggtcgattc ctggcccccg tgctgcgtgg cggtgcttgc gaagacgctc ccgatctttc
     1261 cgacaagggc cccggccgac caaagttggc ccgtggcgtc gaggaactgg cgcatctgcc
     1321 cgcacatgtt tccgaagcgc gtgggggtcc cgaagaagat cgcgtcatag gagggcaact
     1381 cctccaccga gcagacgggg acgtgggcga acgccttctg cgcctcgatc gcccccattt
     1441 tctgaaggac cgccgccgca agcgtctccg gcacgcgccg cagcgtcacc tccgcccccg
     1501 gcacctcccg agccccttcg gccacagctt ccgccatccg gtagacatgc ccatacatcg
     1561 aatagaacac gatcagtgcc ttcatcgcct ctccctccgt gtcggtccgg tttggaacgg
     1621 ggatcccgaa ttgctccacc tttagaatac cccgttcccc tggaaggttc accgcaagga
     1681 agagcgacgt cagcaggttg tcgacaagtt ctcgtggcat aataatatgt tcgacttgaa
     1741 acacccgtgc tgagagggcg gctgacatga cagcgaagag caacccgttt cgcataaatg
     1801 catccggcgt cccgaaaccg accggcctgt acgatccgca gaacgaccac gacagctgcg
     1861 gcgtcgggtt catcgcccgg atcgacggcg tttccctgca caccgtggtc gagcagggga
     1921 tccggatcct cgtcaacctg gagcaccgcg gcgccctggg gggcgacaag tccaccggcg
     1981 acggcgccgg tctcctccta gaggttcccg atacattctt ccgccaggtg tgcccgggag
     2041 gagggctcta ccttcctccg cgcggcgagt acgccgtggg gatgctcttt ctccccatgg
     2101 acgaagcgct cgccgggcgg tgctccgata ccctcgagcg gatcgccgcc gcggaagggt
     2161 gcccggtcct ggggtggcgc gaagtgccgg tcgacccatc gatcctcggg gatctctccg
     2221 ggtccacccg gccgcggatc cgccagctgt tcctcgcgcg cggaccccac gagcgggacg
     2281 ccttcgagcg gaagctctac gtcatccgcc gcctcaccga gaagaaggtc gcctcctggc
     2341 acgacgtcga cgccagccag ttttacatat ccagcctctc cagcaggacg atcgtctaca
     2401 aggggctcct taccggatcc caactcccgc tgttctaccc ggatctgaaa aacgaactct
     2461 tcatgagccc gtacgccgtg gtccaccagc ggtacagcac gaacacgctt cccacctggc
     2521 acctcgcgca cccgttccgg atcgccgccc acaacgggga gatcaacacc ctgcggggga
     2581 acatcaaccg gatgcgggcc cgggaggcaa acctcgcctc gccgctcttc ggcaacgaca
     2641 tcgcaaagct gcgccccgtc atcaacgagg cggggagcga ctccgccatc ttcgacaacg
     2701 ttctcgagct gctcgtgatg tcgggccggt cgctgccgca cgccatcatg atgatgatcc
     2761 ccgaggcgtg gggcgccaag ttccagatga gcgaggacaa gcggtccttc tacgaatacc
     2821 actccgcgat catggagccg tgggacgggc cggcggcgat ggtcttctgc gacgggcggt
     2881 acctcggcgc caccctcgac cggaacgggc tgcgtcccgc gcggtacacc gtgacccgcg
     2941 acgggatgat cgtcctcgcc tccgagaccg gggtgatgga cattccgccg gaccggatcg
     3001 tccgacgcgg ccggctccag ccggggaaga tgcttctcct cgacctgcag cagaagcgga
     3061 tcgtcccgga caacgagatc aaggcggcca tctcccgccg gaagccgtac cggaagtggg
     3121 tcaaggacaa caagatcgag ctgcggggga tgttcaaacc gtccgagatc cctcccgaag
     3181 acccggagac gctccggcgc aagcagcacg ccttcggcta caccgaggag gagatcaagc
     3241 tcatcatcac tccgatggcg tcccaggggc aggaggcgat cggctcgatg ggggacgacg
     3301 cggcgctggc ggtcctctcg aaccggccgc agtcgctctt cgcctacttc aagcagctct
     3361 tcgcccaagt gaccaacccc ccgatcgacc cgctccgcga ggagctggtg atgtcgctga
     3421 acggcttcat cgggagggag cggaaccttc tggacgagac cccggagcat tgccgcatgc
     3481 tccgcctcat ccagccgatc ctcaccccgg aggacatgct ccggcttcgc ggctcgaccc
     3541 acccggacct cgccgccgcc gacctcgaca tgctcttccc ggcgggcggg gacggcaagg
     3601 ccctcgagac ggcgctggcc aggctcttcg tccagggcga caaggcgatc cgggggggag
     3661 cgaccgttct cgtcctgacc gacaggaaca tgaacgccgg ccgcgcgccg atcccgtcgc
     3721 tgctcgccac cgcggggctg caccatcacc tgatccgcag gggtctgcgg acgcaggcgt
     3781 cgatcgtcgt ggaaagcggc gagccgcggg aggtcatcca cttcgcgctc ctcctcgggt
     3841 acggcgccaa cgcgatctgc ccccacacgg tcctctccac gatccgcgag ctggccgaga
     3901 acgaggggct ggaggctccg ctgctggcgg tagaggcggt ggacaaatac gtcacttcgg
     3961 tgaagaaggg gctcctcaag accttcagcc ggatggggat ctccaccctg cgcagctttc
     4021 tcggctccca gatcttcgag gcggtggggc tggggaagga gctggtggac ggctatttca
     4081 cca
//
LOCUS       DASVJL010000014         4680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1596608, whole genome shotgun sequence.
ACCESSION   DASVJL010000014 DASVJL010000000
VERSION     DASVJL010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596608"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VF357_00255"
     CDS             <1..384
                     /locus_tag="VF357_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522603.1"
                     /translation="SPPGTPSWEIAARMAAAVAMVLLMTALARPLGPHLAGLLVPFPV
                     AATVLTVFTHHYQGGASAIRLLRGLVAGLWTMGIFFLVVAGTVERLGVGASFALAVAA
                     DLLSNGGSLWALRRIRAARASGFSS"
     gene            complement(762..1283)
                     /locus_tag="VF357_00260"
     CDS             complement(762..1283)
                     /locus_tag="VF357_00260"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7522604.1"
                     /translation="METRNRSTLFSGLVVNIEQFEVRIGEKGWHTFQIVRHPGGVGVL
                     PLHDDGTVTLIRQLRPAVGGILLEIPAGRLQPGEDPEACGGREMAEETGLSASKLVPL
                     GYFYPSPGVFDEVIHLFLGTGLSQGNAEPEHYEEIATVRMPIGEALRLAASGEIRDGK
                     TIAALLRAGNVVR"
     gene            complement(1310..2272)
                     /locus_tag="VF357_00265"
     CDS             complement(1310..2272)
                     /locus_tag="VF357_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEX7522605.1"
                     /translation="MKAIRVREFGPPEVMRIVEAPDPSPGPGQVLVRIRAAGVNPVET
                     YIRSGAYARRPALPYTPGHDAAGIVEAVGDGVTGVAAGDRVYTSETVTGAYAELALCN
                     AARVHPLPGNVSYAQGAAVGVPYATAWRALFQRARAVPGETVLVHGASGGVGIAAVQI
                     ARAAGLRVFGTAGTAEGVALVLRERAHAAFDHRSAGYKEEAVAATGGRGFDVILEMLA
                     NVNLPWDLKSLAMGGRVVVIGNRGTVEIDPRDAMAREASVVGMSIFNMPDADRAVVNA
                     ALVAGLENGMLRPVIGRELPLSAAPESHVAVMSPGARGKIVLLP"
     gene            2394..2741
                     /locus_tag="VF357_00270"
     CDS             2394..2741
                     /locus_tag="VF357_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX7522606.1"
                     /translation="MDERNDGVMMGAMLVLVGAILGAGGALLFAPQSGRRTRRDISRY
                     ARKTSKMVEGVAGEVVGSVVGLGDAVEEKAEEFLEKGKDLSKESLEAVLTALHEGQER
                     LGRQRDRLAKLLG"
     gene            3089..4306
                     /gene="rpsA"
                     /locus_tag="VF357_00275"
     CDS             3089..4306
                     /gene="rpsA"
                     /locus_tag="VF357_00275"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX7522607.1"
                     /translation="MKPDEEKKETPGEPGEENFGEMLDRSFVAPTRYEPGRKVSARVV
                     KVSPEWVFLDLGRKGEGVLAAKELMDEAGTIAVKEGDTLEAYFVSSDGSEMLFTTRVA
                     GGAAGSAQLEEASNSGIPVDGVVVKEIKGGYEVRLAGGARAFCPHSQMDLPRSGGQEE
                     RVGKHAAFRITKYGEKGRNIVVSRRALLEEEEERKTREVMATLTEGMVMTGTVTAVRE
                     FGAFVSVGPIEGLLPISEIGWDRVEDIQSVLSVGQEVEVAITRLDWANRRFSFSLKKT
                     LADPWETAVDRFPAGSLHVGKVARLAAFGAFVSLGGGVDGLLHISKLGGGKRIRSAGE
                     VLRAGQEIEVKVDSVDPVKRRVALSLPATESADTPEEEAEDYAKYLAPPPAAPALGSL
                     GEALKAKMERKTK"
     gene            complement(4371..>4680)
                     /locus_tag="VF357_00280"
     CDS             complement(4371..>4680)
                     /locus_tag="VF357_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522608.1"
                     /translation="VYRLTRFIGDAMDENVYFNSPLAYLPALSDPWYLDRYRRSRIVV
                     CVGQGAWEDEMLADTRALQAILVSKGIPAWFDYWGHDVNHDWPWWRRQIPYFLELINL
                     "
BASE COUNT          771 a         1517 c         1652 g          740 t
ORIGIN      
        1 tcgccgcccg ggacgccttc gtgggagatc gccgcacgaa tggcggcggc ggtcgcaatg
       61 gtgctcctca tgaccgccct tgcccggccg ctcgggccgc acctcgcggg gcttctcgtc
      121 ccgtttcccg tcgcggcgac ggtgctcact gtcttcacgc accattacca gggcggggcc
      181 tccgcgattc gcctgctgcg ggggctggtc gccggtctct ggacgatggg gatctttttc
      241 ctggtcgtcg cgggaacggt cgagcggctg ggagtcggcg cctcgttcgc gcttgcggtg
      301 gcggcggacc tgctgagcaa cggcggttcg ctctgggccc tgaggaggat ccgggcagcc
      361 cgcgcctctg gattctcttc ctaattgtcg tagcacatca gggcgctttg atcttcgcgg
      421 gatacccctt cctcgctacg ttccggcagc cgtccctggc tgccttgccg ctcccgccat
      481 ccatggctcc tgcggcactt gcccgtccat gggcatcgca cttggagtgt ttttccctcc
      541 cggcccggct ccgatggcca tggatggcca agtgccgcgg gcgcagggat gcgcaggagc
      601 ggccggaaaa aacacacggc cgctcggaag ggtatcccgc tccgatgcgc ctccggggtg
      661 cgtggacgca gggcgcagcg gagaggagaa gcccccccgc gagcccggag ttccctgcgt
      721 cagaatgttg ccgtatttat gaattggtgc accaagggcc cttaccggac gacgtttccg
      781 gcccgcagca gtgccgcgat cgtcttcccg tcccggatct cccccgatgc cgcgaggcga
      841 agcgcttccc cgatcggcat ccgcaccgtc gcgatctcct cgtaatgctc cggctccgcg
      901 ttcccctgcg acagcccggt gccgaggaac aggtggatca cctcgtcgaa gacgccgggg
      961 gaggggtaga aatagccgag ggggaccagt ttgctggcgg atagccccgt ctcctcggcc
     1021 atctccctgc cgccgcacgc ctccggatcc tcgcccggtt gaagtctgcc cgccgggatc
     1081 tcgaggagga tgccgcctac cgccggacgc agctgccgga tcagcgtgac cgtcccgtcg
     1141 tcgtgcagcg ggaggacgcc gacgccgccc gggtgccgca cgatctggaa ggtgtgccac
     1201 cccttctccc cgatccggac ctcgaactgc tcgatgttca cgaccagccc ggaaaagagc
     1261 gtcgaccggt ttctcgtctc catgcggccc ctttccgctt tgctccaggt caaggaagga
     1321 ggacgatctt tcccctggcg ccgggcgaca tcacggccac gtggctctcc ggcgccgcgg
     1381 acagcggcag ttcccggccg atgacaggcc gaagcatccc gttctcgagc cccgcgacga
     1441 gggcggcgtt gacgacggcc cgatcggcgt ccggcatgtt gaagatggac atcccgacga
     1501 ccgaggcttc ccgcgccatc gcatcccgcg ggtcgatctc cacggttccc cggttgccga
     1561 tcacgacgac ccgcccgccc atcgcgagag acttgagatc ccacggcagg ttcacgttgg
     1621 cgagcatttc caggatgacg tcgaatcccc ggccccctgt cgccgccacg gcctcttcct
     1681 tgtatcccgc cgaacggtgg tcgaaggcgg cgtgcgccct ctcacgcagg accagggcga
     1741 ccccctcggc ggtccctgcc gtgccgaaga cgcgcagtcc cgcggcccgg gcgatctgga
     1801 ccgccgcgat ccccacgccg ccgctcgccc cgtggaccag aaccgtctct cccggtacgg
     1861 ctcgcgctcg ctggaagagg gcgcgccagg cggtggcgta cggtacgccg accgcggccc
     1921 cctgcgcgta cgagacgttg ccgggaagcg ggtggacccg tgcagcgttg cacagcgcaa
     1981 gctccgcgta ggccccggtc accgtctccg aggtgtacac gcggtcgccc gcggcgaccc
     2041 ccgtgacacc gtctcccacc gcctcgacga tgcccgcggc atcgtgcccg ggggtgtagg
     2101 ggagcgccgg gcgtcgggca tacgccccgg agcggatata cgtctccacc gggttcaccc
     2161 ccgcggcccg tatacggacc agcacctggc cgggtcccgg cgacgggtcc ggggcctcca
     2221 cgatgcgcat cacttccggc ggaccgaatt cgcgaacacg gatcgctttc atgtaggttt
     2281 cctcctgcgg taattgtacc cgatgagatg cctcccgccc tcccgctgtt tcgccgggcg
     2341 tctccggggt acactactac gagtgagacg agaaacgata ccggagggga cccatggacg
     2401 agaggaacga cggcgtgatg atgggagcga tgctggtgct cgtaggtgcg attctcgggg
     2461 ccggcggagc cttgctgttc gcgccgcaat ccggacgtag aacgcgcagg gacatctccc
     2521 ggtatgcccg gaagacgagc aagatggtgg aaggcgtggc gggggaagtc gtcgggagcg
     2581 tggtcgggct gggcgacgcc gtggaggaaa aggcggagga attcctcgag aaggggaagg
     2641 atctctccaa ggagtccctg gaggcggtgc tcaccgccct gcacgaggga caggagcggc
     2701 tgggacggca gcgggaccgg ttggcgaaat tgctggggta gcgtctcgca aatagcctga
     2761 gcatcgagag cgtcgatgcg ccgcgccagc gaggcgcgcg agtgaaggcg taccgggaga
     2821 gtacggcgaa cttgccgccc cactcccgga tgaaatacgc tccctggggt accccggaca
     2881 gccgatgcca tgtaccttgt ggtcggcatg tggggcacaa cgaagctgga ggcggcagcg
     2941 acggccatgg atggccaagt gccgcggagg gcatggatgc ccgggagcgg ccggcgctcg
     3001 aatgccaggg tatttgcggg acgctgcccc aaggtagctt ccggtcaaga cgcggaggcg
     3061 gcggagaaaa acaggacggg gggaacgcgt gaagccggac gaggagaaga aggaaacgcc
     3121 gggcgaaccg ggagaggaga atttcgggga gatgctcgac aggagcttcg tcgccccgac
     3181 gcggtacgag cccgggcgga aggtatccgc ccgggtcgtc aaggtgtccc cggagtgggt
     3241 cttcctcgac ctgggtcgga agggagaagg tgtccttgcg gcgaaggagc tgatggacga
     3301 ggcgggcacc atcgccgtca aggaggggga caccctcgag gcgtacttcg tctcctcgga
     3361 cggcagcgag atgctcttta ccacccgcgt cgccgggggg gccgccgggt cggcgcagct
     3421 cgaggaggcc tcgaactcgg ggattcccgt ggacggggtc gtggtgaagg agatcaaggg
     3481 gggatacgaa gtccgcctcg cgggaggggc gagggcgttc tgcccccact cgcagatgga
     3541 ccttccgcgg tccggaggtc aggaggagcg cgtcgggaag cacgccgcgt tccggatcac
     3601 gaagtacggg gaaaagggtc gcaacatcgt cgtctcccgc cgggccttgc tggaagagga
     3661 ggaggagcgg aaaacgcggg aggtgatggc gacgctcacg gaggggatgg tgatgacggg
     3721 gaccgtcacc gcggtccggg agttcggcgc cttcgtctcc gtcggcccga tcgaggggct
     3781 gctccccatt tcggagatcg ggtgggacag ggtggaggat atccagagcg ttctctccgt
     3841 gggacaggag gtcgaggtcg cgatcacgcg cctcgactgg gcgaacaggc gcttctcctt
     3901 cagcctcaag aagaccctcg cggacccgtg ggaaaccgcc gtcgaccgtt tcccggcagg
     3961 gtcactccac gtgggcaagg tcgcccgcct ggccgccttc ggggcctttg tctccctcgg
     4021 cgggggagtc gacggcctgc tccatatatc gaaactcggc gggggaaagc ggatccggag
     4081 cgcgggcgag gttcttcgag cggggcagga gatcgaagtg aaggtggatt cggtcgaccc
     4141 cgtgaagcgt cgcgtcgccc tctcccttcc ggcgacagag agcgcggaca cgcccgaaga
     4201 ggaagcggag gattatgcga agtatctcgc tccgccgccc gccgcccctg ccctcggttc
     4261 cctgggggaa gccctcaagg cgaaaatgga gcggaaaacg aaataaccgg aggcggagcg
     4321 tagcgaggaa gggggtatcc cgcgaagata aacgcctccg gctacatcgg ctacaggttg
     4381 atgagttcga ggaagtacgg tatctgacgg cgccaccatg gccagtcgtg attgacgtcg
     4441 tgcccccagt agtcgaacca tgcgggaatc cccttcgaca cgaggatcgc ctgcagggcg
     4501 cgggtgtccg cgagcatctc gtcctcccag gctccctgcc cgacgcacac cacgatccgg
     4561 ctgcggcggt accggtccag gtaccacggg tcggaaagag ccgggaggta ggcgagaggc
     4621 gagttgaagt agacgttctc gtccatggcg tccccgatga accgggtgag cctgtacacg
//
LOCUS       DASVJL010000015         3759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_96367, whole genome shotgun sequence.
ACCESSION   DASVJL010000015 DASVJL010000000
VERSION     DASVJL010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96367"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VF357_00285"
     CDS             <1..358
                     /locus_tag="VF357_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522609.1"
                     /translation="AAPKGCASVFVETSFPSGARVNVGKEVRKATDGLRRMGVLPHGR
                     KPEVVEPVLLDPAYVVFDHARDAAVRRVREHLREKGVFTAGRYGAWEYYGMEASMADG
                     IRAARDAMHKVRGMSA"
     gene            355..1557
                     /locus_tag="VF357_00290"
     CDS             355..1557
                     /locus_tag="VF357_00290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7522610.1"
                     /translation="MSGTVNRGGTARARVVHVITMLEWGGAQENTLYSVGHLDPGRYD
                     RVLVAGKGGMLDGRARRIPDLRVVLLEDLVREVRPARDVKAFLALRSILREEKRVAGG
                     DPLVVHTHSSKAGILGRVAARAAGADAVVHSIHGFGFHDGQSKPTHALYVGLEKMASR
                     WTDAFVAVSEETIRAGVQEGIFVRERCRLIRSGFDTARFLAGSRAVGRKILGIPEGVP
                     VVGTVAVFKPQKAPLDFVETARRVASEIPDSRFVMVGDGELRGEAERAVAAIGMSDRF
                     SFLGWRPEVPELMAAFDVFLLTSRWEGLPKVVPQALIAGCPVVATAVDGTREIVDHGV
                     DGVLAPPGDVDALARGVSDILAGRLPLDVTRKRERLVREFDQDEMVRAQERLYAEILA
                     GKGLPGWS"
     gene            1554..2303
                     /locus_tag="VF357_00295"
     CDS             1554..2303
                     /locus_tag="VF357_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX7522611.1"
                     /translation="MMAVVAFLVGACIGSFLNVVIHRLPRGESIVSPRSRCPGCGRAI
                     RAWENIPIVSFIVLGGKCAGCGGAISWRYPAVELLTATGFAAIFLLDGPGIPVLRDLL
                     FFSLLVPIAFIDIDHRIIPDELSLGGLAAGLLLSFLPGGEWKRSVAGALLGGGILYAT
                     AFLYEKVRGTEGMGGGDIKLLAMIGAFVGWRGTLATIFFGAFLGVAGGILAMRKGGAG
                     LKTAIPFGPYLCVAALLSRYLGDRFWGMILF"
     gene            complement(2339..>3759)
                     /locus_tag="VF357_00300"
     CDS             complement(2339..>3759)
                     /locus_tag="VF357_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX7522612.1"
                     /translation="DRVRPWDVLKLEAVLESGPVHLPRELDRSALQYYLEFQYVPGPH
                     AIYRDVEKVLPGHIIEIGGEGKITARAYWDLFDHWGKAEGRTRTEGECEEELSALLDS
                     SIRYRMISDVPLGAFLSGGIDSSLVVALMRKTASGPVKTFSIGFRESGYDESPHARAV
                     AAHLGTDHHEKICTPQEAQALVRRIPDAYDEPFADSSAIPTMLVSEFTRQHVTVSLSG
                     DGGDELFCGYPRYAWVRQGNVVRGIPGFLRRPLCSLLSRVPIHKVQRGAESVLYDDPA
                     EMYFHTVGIFERRRLGEIVPGVVDDSHLPYFRTFRDPRCGGIVERAMATDIKTYLVDD
                     ILTKVDRASMAYSLEARVPLLDHRIVEFAARLPTERKVQSGGTKHLLRKILYRHVPRE
                     LIDRPKMGFGIPVNRWLRKELRPLLDEYLGDDRVRREGFLRPEGVEQVVREHLSGRRD
                     HQYRLWALLVFGMWVERYRPTS"
BASE COUNT          565 a         1176 c         1335 g          683 t
ORIGIN      
        1 cgcggcgccg aaaggatgcg cgtccgtgtt cgtggagacg agcttcccct cgggagcacg
       61 ggtaaatgtc gggaaggaag ttcgtaaggc gacggacggc ctgcgacgga tgggagtcct
      121 tccacacggg cggaaacccg aggtcgtgga acctgtcctt ctcgaccccg catacgtcgt
      181 gttcgatcat gcccgggatg cggcggtgcg acgggtgcga gagcatcttc gcgaaaaggg
      241 agtcttcacc gcgggtcggt atggcgcctg ggagtattac ggaatggagg cgtcgatggc
      301 ggatgggatc cgcgccgccc gggatgccat gcacaaggtt cggggaatgt ccgcgtgagc
      361 gggacggtga atcgcggcgg aacggcccgc gcccgcgtgg tccacgtcat cacgatgctc
      421 gagtgggggg gcgcccagga gaacaccttg tacagcgtgg ggcacctcga tcccgggcgg
      481 tacgaccgcg ttctggtcgc cgggaaaggc gggatgctcg acgggcgggc gcgccggatc
      541 cccgatctgc gggtcgtcct gctggaggat cttgtccgcg aggttcgtcc cgctcgcgac
      601 gtgaaggcgt tccttgcatt gcgatcgatc ctccgggagg agaagcgcgt ggccggcgga
      661 gatcccttgg tcgtgcacac ccacagttcc aaggcgggga tcctcggaag ggtggcggcg
      721 cgcgcggcgg gagcggacgc cgtcgttcac tccattcacg ggttcgggtt ccacgatggc
      781 caatcgaagc cgacgcatgc tctctacgtc gggttggaga agatggcgtc ccgctggacg
      841 gacgcgttcg tggcggtgtc ggaggagacc atccgggcgg gcgttcagga ggggatcttc
      901 gttcgggagc ggtgccgcct catccgcagc ggcttcgaca cggcgagatt cctcgcgggg
      961 tcccgcgcgg tgggccggaa gatcctgggg attccggaag gggtcccggt ggtgggaacg
     1021 gtggcggtgt tcaagccgca aaaagccccg ctggatttcg tggagacggc ccggcgcgtg
     1081 gcgtcggaga tccccgattc ccggttcgtg atggtcggcg acggggaatt gcgcggagag
     1141 gcggagcggg ccgtcgccgc gatcgggatg tcggatcgtt tttcgttcct cggctggcgg
     1201 cccgaggtcc cggagttgat ggcggccttc gacgtgttcc ttctcacttc ccggtgggaa
     1261 ggccttccga aggtggtgcc gcaggcgctg atcgccgggt gcccggtggt tgccacggcg
     1321 gtcgacggga cccgggagat cgtcgatcac ggggtggatg gtgtgctcgc ccctccgggg
     1381 gacgtcgatg ccctcgcccg cggcgtctcg gacatcctgg cgggacggct tccgctggat
     1441 gtgacgagaa agcgggagcg cctggtccgg gagttcgatc aggacgagat ggtgcgggcg
     1501 caggagcggc tctacgcgga gatcctcgcg ggaaaggggc ttccgggttg gtcatgatgg
     1561 cggtagtggc gtttctcgtc ggggcgtgca tcggaagttt cctgaacgtg gtgattcatc
     1621 ggttgccgcg aggggagtcg atcgtatcgc cccgatcccg ctgccccggc tgcggcaggg
     1681 cgatccgggc gtgggagaat atcccgatcg tgagcttcat cgtcctcgga gggaaatgcg
     1741 ccgggtgcgg cggggcgatc tcctggcggt acccggcggt cgagctgctc acggcgaccg
     1801 ggttcgccgc gatctttctt ctggacgggc ccgggatccc ggtgttgcgc gacctgctct
     1861 tcttctccct cctcgtgccg atcgccttca tcgacatcga ccaccgaatc atccccgacg
     1921 aactctccct cggtgggctc gccgcgggac tgctcctttc cttcctcccc ggcggggagt
     1981 ggaagaggag cgtcgcgggt gcgctgctgg gaggcggcat cctctacgcg acggccttct
     2041 tgtatgaaaa agtgcggggg accgagggga tgggcggcgg ggacatcaaa ctgttggcga
     2101 tgatcggtgc cttcgtcgga tggcgtggca cgctcgcgac gatcttcttc ggcgcgttcc
     2161 tcggtgtggc gggggggatc ctggcgatgc gaaagggcgg ggcggggctg aagacggcga
     2221 tcccgttcgg gccgtacctg tgcgtcgccg cccttctgtc ccggtatctc ggcgatcggt
     2281 tttgggggat gatcctgttc tgaacggcga ggtattgtcc cgggacggac aggtccggtc
     2341 aggacgtcgg acggtagcgc tccacccaca ttccgaagac cagcagcgcc cacagccggt
     2401 actggtggtc ccggcgcccc gacaggtgct cccggacgac ctgctccacc ccttccggtc
     2461 ggaggaatcc ctccctccgc acccgatcat cgcccaggta ctcatcgagc agcgggcgca
     2521 gttccttgcg cagccaccgg ttgaccggga tgccgaaccc cattttcggg cggtcaatca
     2581 attcccgcgg cacgtgtcgg tagaggatct tccgcaggag atgcttcgtt cccccgcttt
     2641 gcaccttgcg ctccgtgggg agtcgtgccg cgaactccac gatccggtga tcgagcagcg
     2701 gcacccgcgc ctccagcgag tatgccatcg aagcgcggtc gactttcgtc aggatgtcgt
     2761 ccaccaggta cgtcttgatg tcggtggcca tcgcgcgctc gacgatcccc ccgcagcggg
     2821 gatcacgaaa cgtccggaaa tatggaagat gggagtcgtc gaccaccccc gggacgatct
     2881 ccccgaggcg tcttcgctcg aagatcccca cggtgtggaa gtacatctcc gccgggtcgt
     2941 cgtacaggac gctctccgcc ccccgctgca ccttgtggat cgggacgcgg gagaggaggg
     3001 aacacaacgg gcgccgaagg aaccccggaa tcccccgcac cacgtttccc tggcggaccc
     3061 acgcatagcg cgggtacccg cagaacaact catccccccc gtcccccgac aggctgacgg
     3121 tgacgtgttg ccgcgtgaac tccgagacca gcatcgtcgg gatggccgac gagtcggcga
     3181 acggctcgtc gtaggcatcg gggattctcc ggaccagcgc ttgcgcctcc tggggggtgc
     3241 agatcttctc gtggtggtcc gtcccgaggt gcgccgccac cgcccgcgcg tgcggtgact
     3301 cgtcgtatcc gctctcccgg aagccgatgg agaacgtctt cacggggccc gatgcggttt
     3361 tcctcatcag cgccacgacg agggaggagt cgatcccccc ggagaggaac gcccccagcg
     3421 gcacgtcgct gatcatccgg taccggatgg aggagtcgag cagggcggag agttcttcct
     3481 cgcactcccc ctcggtcctc gtacgccctt ccgctttccc ccagtgatcg aacaggtccc
     3541 agtaggcgcg ggctgtgatc ttcccttcgc cgccgatctc gatgatgtgc cccgggagca
     3601 ccttctccac atcccggtag atcgcgtgcg gccctgggac gtactgaaac tcgaggtagt
     3661 actgaagggc ggatcggtcc aattcccgcg gaaggtggac cggtccgctc tccagtactg
     3721 cctcgagttt cagtacgtcc cagggccgca cgcgatcta
//
LOCUS       DASVJL010000016         4065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_2775, whole genome shotgun sequence.
ACCESSION   DASVJL010000016 DASVJL010000000
VERSION     DASVJL010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2775"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VF357_00305"
     CDS             complement(<1..663)
                     /locus_tag="VF357_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX7522613.1"
                     /translation="MNVTYHRWWSRNIGQDMELKVYGHGGKPVVVFPSSGGRFHEYED
                     FGMVEACRSFIDAGAVTLFTLDSVDRQSWLNEGAHPADRARRHNDYDRYVVEEAAPFI
                     RGLFPGATGFLATGCSMGGYHSANFFFRHPDVFDAMIALSGVYNLTRFIGDAMDENIY
                     FNTPLAYLPALSDPWYLDRYRRSRIVVCVGQGAWEDEMVADTRDLQAILASKGIPAWI
                     DFW"
     gene            complement(686..1849)
                     /locus_tag="VF357_00310"
     CDS             complement(686..1849)
                     /locus_tag="VF357_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7522614.1"
                     /translation="MNFVYLSPHFPPNYYRFCVRLREEGVNVLGLADAPYEALRPELR
                     GALSEYYRVDDMNSYDALVRALGHFTHRHGKIDGIDSNSEHWLETEARLRTDFNMAGL
                     QQDRIGIIKRKSAMKEIYRAAGIPVARGAVARTLEEARRLVAGTGYPVIAKPDVGVGA
                     AATFKIRDDGELSSFFDRKPAVDYIVEEFIQGKIFSFDGLTDRDGNLVFFTSHAFSQG
                     IMETVNEDADLFYYSLREIPADLEEAGRRTARAFDVRGRFFHFEFFRADRDGGIVALE
                     VNLRPPGGLTTDMFNYANDIDIYREWACVVARNRFTAEWSRPYHVGFVGRKRGKRYVH
                     GPGEIGAVVGGLIVHYEEIDSVFRAAIGDYGYLVRSKDLEEVLAAARYIQETA"
     gene            2220..2507
                     /locus_tag="VF357_00315"
     CDS             2220..2507
                     /locus_tag="VF357_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX7522615.1"
                     /translation="MRVVNRTRGTVVGDRVRTARTFLSRLVGLLGTAAIADGEGLWIV
                     PCRSVHTLEMRYPIDVAFLDARGALELRSGILAATGTVPGDRLEFEGGGPA"
     gene            2599..3624
                     /gene="hrcA"
                     /locus_tag="VF357_00320"
     CDS             2599..3624
                     /gene="hrcA"
                     /locus_tag="VF357_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX7522616.1"
                     /translation="MDNRTTRVLRHIVEDYIATAEPVGSRTISKKMGQSLSPATIRNI
                     MADLEEAGYLAQPHTSAGRVPTGAGFRYYIDHLLARQSLARGEMDQLHRAAGEGNGPA
                     DELVRQVSRLLSNLAHQASVVVVSSPEQQVLSSVSLMRAGVERILLVTVMRGGWVQHR
                     LIEGEPDLTGDELEKFSAYLNELAEGRTLLQLRARILDELGKEKARYDRVMGRALTMS
                     ARALTDTAPGEVFIEGRANILEQPEFAEDVHRLKRILRAFEEKSVIFRLLDRAMESRA
                     IQVSVGLENAVEELPDISVVASGYRQGASSVGSIGLIGPVRMDYSRVIPLVEYAASLL
                     TTMFEDR"
     gene            3668..>4065
                     /locus_tag="VF357_00325"
     CDS             3668..>4065
                     /locus_tag="VF357_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX7522617.1"
                     /translation="MEDREKESPLVETEAEEIEAGPASSGEPGETVDAATLKEQLAYL
                     AAEFENFRKRAMREREAQAAFGNEQLLRAVLPFLDNLERAMDQTSSSVGALLSGVRMT
                     HDLFLSELRKFGLEQLPAQGGNFDPSLHEA"
BASE COUNT          722 a         1330 c         1341 g          672 t
ORIGIN      
        1 ccagaagtcg atccatgcgg ggatcccctt cgacgcgagg atcgcctgca ggtcgcgggt
       61 gtccgcaacc atctcgtcct cccatgcccc ctgcccgacg cacaccacga tccggctgcg
      121 gcggtaccgg tccaggtacc acgggtcgga aagcgccggc aggtaggcga ggggcgtgtt
      181 gaagtagatg ttctcgtcca tggcatcccc gatgaaccgg gtgaggttgt acacgccgga
      241 cagggcgatc atggcgtcga agacatcggg gtggcggaag aagaagttcg cggagtgata
      301 tccccccatg ctgcaccccg tcgcgagaaa tcccgtcgcg ccggggaaca ggccccggat
      361 gaacggggcg gcctcctcga ccacataccg gtcgtagtcg ttatgccttc gcgcacggtc
      421 cgcgggatgg gccccctcgt tcagccacga ctggcggtcc acgctgtcga gggtgaagag
      481 ggtgacggcg cccgcgtcga tgaaggaccg gcacgcctcg accatcccga aatcctcgta
      541 ctcgtgaaat cgcccgccgg aggaggggaa gacgaccacc ggtttccccc cgtggccgta
      601 caccttgagc tccatgtcct gcccgatgtt ccggctccac catctgtggt acgtgacgtt
      661 catcgaaggt cccctcgtct ccgagttacg cggtttcctg aatgtatcgg gcggcggcca
      721 gcacctcttc gaggtccttc gaccggacca ggtacccgta gtccccgatc gccgcgcgga
      781 agacggagtc gatctcctcg tagtgcacga tgaggccccc gacgaccgca ccgatctccc
      841 cgggaccgtg gacgtaccgc ttcccccgct tccggccgac gaagccgaca tggtacggtc
      901 gggaccactc cgccgtgaag cggttgcggg ccaccacgca cgcccactcc cggtagatgt
      961 cgatgtcgtt ggcgtaattg aacatatccg tggtcaagcc ccccggcggc cgcaggttca
     1021 cctcgagtgc gacgatcccg ccgtcccgat cggcccggaa gaactcgaag tgaaagaacc
     1081 gtccccggac gtcgaaggcc cgcgccgtcc gccgccccgc ctcctccagg tccgccggga
     1141 tctcccggag ggagtagtaa aagaggtcgg cgtcctcgtt gaccgtctcc atgatcccct
     1201 ggctgaaggc atgcgaggtg aagaagacga ggttgccgtc gcggtcggtc aagccgtcga
     1261 aggagaagat tttcccctgg atgaactcct ccacgatgta gtccaccgcc ggcttccggt
     1321 cgaagaagga ggagagctcg ccgtcgtcgc ggatcttgaa ggtcgccgcc gctcccaccc
     1381 ccacgtcggg cttggcgatc accgggtagc cggttccggc gaccagccgt cgcgcctcct
     1441 cgagggtccg ggccaccgcg ccgcgggcga ccgggattcc ggcggcccgg tagatttcct
     1501 tcatggcgga cttccgcttg atgatcccga tccggtcctg ctgaagcccg gccatgttga
     1561 agtccgtccg aaggcgcgcc tcggtctcca gccagtgctc gctgttggag tcgatcccgt
     1621 cgatcttccc gtggcggtgg gtgaagtgcc cgagcgcccg gaccagcgcg tcatacgagt
     1681 tcatgtcgtc cacgcggtaa tattcggaga gcgccccgcg gagctccggc cgcagcgcct
     1741 cgtacggggc gtcggccagc ccgaggacgt tcaccccttc ctcccggagg cgaacgcaga
     1801 agcggtagta gttcggcgga aaatgcgggg agagatagac gaaattcatc gactcatcgt
     1861 cgcaggcccg gcaggaaccg tcaagcagtg acgggtgacc ccgagtgccg cgtttcacaa
     1921 atacggcaac gtaaccggag gcgcatttat ctccgcgtga tactcccttc ctcgctacgt
     1981 tccggcagcc ctccctggct gccttcactg gccgctcccg ccatccatgg ctcccgcggc
     2041 acttggccat ccctggccat cgacgatggt agcggagagg aagccccccc ctacgagctg
     2101 cgcgctggtg gggtaccccg ccgcaggaaa aatggggggc acgagcccgg agtccactgt
     2161 atatggacag gagcacctgg tttgctattc ttgacatcga acaaagcacg gaggattcgg
     2221 tgcgggtcgt caaccggaca cgaggaacgg tcgtcgggga tcgggttcgg acggcgcgaa
     2281 cgttcctgtc gcgccttgtg gggttgctcg gaacggccgc gatcgcggat ggcgaggggt
     2341 tgtggatcgt cccctgccgc agcgtgcaca ccctggagat gcggtacccg attgacgtgg
     2401 cgttcctcga tgcccggggg gcgctggagc tgcgctcggg gatcctcgcc gccaccggca
     2461 ccgtgcccgg cgaccggctg gagttcgaag ggggcggacc ggcttgacac aatcggccga
     2521 cgactgctac attgttcatt agcactctcc ttgcaggagt gctaactcat tcgcggagaa
     2581 ggtgaaatgg cttccgggat ggataatcgc acgacccggg tcctacgcca catcgtcgag
     2641 gattacatcg ccacggcgga gccggtcgga tcaaggacca tctccaagaa gatggggcag
     2701 tccctttcgc ccgcgacgat ccgcaacatc atggcggacc tcgaggaggc cgggtacctc
     2761 gcccagccgc acacctccgc ggggcgcgtc cccacagggg ccgggttccg gtactacatc
     2821 gaccacctcc tggcacggca atccctcgcc cgcggcgaga tggaccagct gcaccgcgca
     2881 gcgggcgaag ggaacggtcc ggcggacgaa ctggtgcggc aggtcagccg cctgctgtcg
     2941 aacctggccc accaggcgag cgtcgtcgtg gtttcctcgc cggaacagca ggtcctcagc
     3001 tccgtgagcc tcatgcgcgc tggagtggag aggatccttc tggtcaccgt gatgcgcgga
     3061 ggatgggtac agcaccgcct gatcgagggc gagccggatc tcaccggaga cgagctcgag
     3121 aagttcagcg catacctcaa cgagctggca gaggggcgga cgctgctgca gctgcgcgcc
     3181 cggatcctcg acgagctcgg caaggagaag gcccggtacg accgcgtgat ggggcgtgcc
     3241 ctcacgatga gcgcccgggc gctcacggac accgccccgg gagaggtctt catcgagggc
     3301 cgcgccaaca tcctcgaaca gcccgagttc gccgaggacg tccaccgcct gaaacggata
     3361 ctgcgtgcct tcgaggagaa gagcgtgatc ttccgcctgc tcgaccgggc gatggagagc
     3421 cgggcgatcc aggtgtccgt cgggctcgag aacgcggtgg aagagcttcc tgacatttcg
     3481 gtagtcgcct cgggataccg acagggcgct tcctccgtgg ggagcatcgg cctcatcggg
     3541 cccgtccgga tggactactc ccgggtgatc ccgctggtcg agtacgccgc gagcctgctc
     3601 accacgatgt tcgaggaccg ttagcccgcc cggatttccc gcgcaatttc accaggagag
     3661 gaaagggatg gaagacaggg agaaagaatc gccgctggtg gaaaccgagg cggaggagat
     3721 cgaagcgggg ccggcgagca gcggggaacc cggggaaacg gtcgatgcgg caacactgaa
     3781 ggaacagctg gcataccttg ccgcggagtt cgaaaatttc cggaagcgcg caatgcggga
     3841 gcgggaggcg caggcggctt tcgggaacga acagctgctg cgcgccgttc tgccgtttct
     3901 cgacaacctc gagcgggcga tggaccagac gagttcctcc gtcggtgctc tcctctccgg
     3961 cgtccggatg acgcacgacc tgtttctctc ggaattgcgc aaattcggtc ttgagcagct
     4021 ccccgcgcaa ggcgggaatt tcgacccgtc cctgcacgag gcgat
//
LOCUS       DASVJL010000017         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1690982, whole genome shotgun sequence.
ACCESSION   DASVJL010000017 DASVJL010000000
VERSION     DASVJL010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690982"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..2032
                     /locus_tag="VF357_00330"
     CDS             197..2032
                     /locus_tag="VF357_00330"
                     /inference="COORDINATES: protein motif:HMM:NF009232.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HEX7522618.1"
                     /translation="MDFAPLDLAPAEIDVRKTPEGGYLLRSPNALAPYEESLGVMFRR
                     WVGESPDRTFLGERDASGTWRTVTWGEAGRLADSVAQALIDRRMDSERPVMILSGNSI
                     DHALLMLGGFIAGVPLVPVSVAYSLLSQDFAKLKSILREVRPALVFVENAEMFSRALK
                     ALDLAGVEVVAGRGSVEGIPVTPFSSFLATVPTSLVAKAFVAVRPGSVAKVLFTSGST
                     GLPKGVINTHGMLCSNQQMLAQIWPFTRATPPVLVDWLPWNHTFGGNHDFNLVLKRGG
                     TLYVDGGKPAPGLVEQTVRNLAEISPTIYFNVPAGFAMLLPFLERNAKLRDSFFRRLQ
                     LVFYAGAALSQDLWQRLEAVSLQATGRKVPMTSSWGSTETAPLATSAHFPIDKAGVIG
                     IPCPGVTIKMVKSGVKYELRVKGPNVTPGYLHRPDLTRAAFDAEGFYRIGDAGTFADP
                     DNPGRGIVFAGRVAEDFKLSTGTWVHVGNLRLAVMAAAAPALQDALVAGHDREAIGIL
                     AWPNLQWLREICRDTGGCRPVEDLVRDKEVLDHLRNSLTEYNVRQQGSATKVGRILLM
                     AEPPSIDANEITDKGYINQGAALERRGDLVTRLYAERPDSGVVVL"
     gene            2085..>2297
                     /locus_tag="VF357_00335"
     CDS             2085..>2297
                     /locus_tag="VF357_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7522619.1"
                     /translation="MKKFLRFLAGAAVLMFAASAGIAADTIKVGFVDTYSGPATTFTN
                     DVLDGFKMAAEKINAKGGVLGRKITYV"
BASE COUNT          372 a          734 c          780 g          411 t
ORIGIN      
        1 tcccgctgcg gtgcctacgc gctttcgggc ggcgcactcg cttcgtgcct ggagctggtt
       61 tcccgccgtt ccgtattccc ggtcagtggg ttgctggagg cgaagcgcct ccttgcggag
      121 gcgtcgctgg aggacggcag atatgcgttc cggtcccatg aaacctgagg aaacggggaa
      181 gataccggtg ggaggaatgg atttcgcgcc gctggatctg gcgccagccg aaatcgacgt
      241 gcggaagacc ccggagggag ggtatctcct ccgttcaccg aatgcgctcg cgccgtacga
      301 ggagagcctg ggggtgatgt tccgccgttg ggtcggggag tcccccgacc ggaccttcct
      361 cggagagcgg gacgcctcgg gtacgtggcg gaccgtgacg tggggggagg ccggccgcct
      421 cgccgattcc gtggcgcagg cgctgatcga tcgccggatg gattccgagc ggccggtaat
      481 gatcctctcc gggaactcga tcgatcacgc cctcctcatg ctcggggggt tcatcgccgg
      541 cgtgccgttg gtcccggtct ccgtggcgta ctcgctgctg agccaggact tcgccaagct
      601 caaatcgatt ttgcgggagg tccgtccggc gctcgtcttc gtcgaaaacg cggagatgtt
      661 ctcccgcgcg ctcaaggcgc tggatcttgc cggtgtcgag gtcgtcgccg gccgcggctc
      721 ggtcgaaggg atccccgtca cccctttttc ctcgttcctc gcgacggtcc caacttcgct
      781 agtcgcgaag gcgttcgtcg cggttcgccc gggatcggtc gccaaggtgc tcttcacttc
      841 cggttcgacc ggcctcccga agggggtgat caacacccac gggatgctct gctccaacca
      901 gcagatgctg gcgcagatat ggccgttcac gcgggctacc ccgcctgtcc tggtcgactg
      961 gctcccgtgg aaccacacct tcggcgggaa ccatgatttc aacctcgtcc tcaagcgcgg
     1021 gggaaccctc tacgtcgacg gtggcaagcc ggcccccggc ctcgtggagc agacggtcag
     1081 gaacctcgcg gagatttccc cgaccatcta cttcaacgtg ccggccggct tcgccatgct
     1141 cctccccttt ctcgagagga acgcgaaatt gcgggacagc tttttccggc ggttgcagct
     1201 cgtcttctac gccggcgccg ccctttcgca ggacctctgg cagcgcctgg aagcggtctc
     1261 cctgcaagct accgggcgga aggtcccgat gacctcctcc tgggggtcga cggaaaccgc
     1321 ccccttggcc acctcggccc acttcccgat cgacaaggcc ggcgtcatcg ggatcccgtg
     1381 ccccggggtg accatcaaga tggtgaaaag cggcgtgaag tacgagctcc gggtgaaggg
     1441 gcccaacgtg acgcccgggt acctgcaccg tccggacctg acgcgcgccg ccttcgatgc
     1501 cgaagggttc tacaggatcg gcgacgccgg gacgttcgcc gacccggaca atcccggccg
     1561 tgggatcgtc ttcgcgggcc gggttgcgga ggatttcaag ctctctaccg ggacctgggt
     1621 gcacgtgggg aacctgcgcc tcgccgtgat ggcggcggcg gcgccggcct tgcaggacgc
     1681 cctggtggca gggcacgacc gggaggcgat cgggatcctg gcctggccga acctccagtg
     1741 gctgcgggag atctgccggg acaccggggg gtgccggccg gtggaggatc tcgtccgcga
     1801 taaagaggtt ctcgaccacc ttcggaactc gcttacggag tacaatgtac ggcagcaagg
     1861 atccgcgaca aaggtcgggc gcatcctgct catggcagaa ccaccatcga tcgacgcgaa
     1921 cgagatcacc gacaagggat acatcaacca gggagccgcg ctggaacggc gcggggatct
     1981 ggtgacgcga ctgtacgcgg agcgtccgga tagcggagtg gttgtcctgt gaccggtccg
     2041 atccggagcc acccacgcga ttattccata agggggagga gacggtgaaa aagttccttc
     2101 ggtttctggc tggcgccgcg gtactgatgt tcgcagcctc ggcggggatt gcggcggaca
     2161 ccatcaaggt cgggttcgtc gacacgtatt ccggcccggc gacgaccttc accaacgacg
     2221 tcctcgacgg gttcaagatg gccgcggaga agatcaacgc gaagggcggc gtgctcggca
     2281 ggaagatcac gtacgtg
//
LOCUS       DASVJL010000018         6333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_377616, whole genome shotgun sequence.
ACCESSION   DASVJL010000018 DASVJL010000000
VERSION     DASVJL010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377616"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VF357_00340"
     CDS             <1..364
                     /locus_tag="VF357_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEX7522620.1"
                     /translation="VERAEPLLTVPPLKPLIDIPRMADIAQEMLKASLDAFVNGDDEL
                     AFATILRDDLVDQLKDQVFRELLTFMMADPTTIPRAMDLILVSRHLERIADHATNICE
                     DVIFMVKGKDVRHQGPLA"
     gene            384..1922
                     /locus_tag="VF357_00345"
     CDS             384..1922
                     /locus_tag="VF357_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX7522621.1"
                     /translation="MARIAAIDMGSNAIRFYVVETGGDSSYRVLENVREPIRLGGDVF
                     LKGTIREENIRKAEAAFRRFRQLLKAHGVQTVRAVATCATREASNADLLLSRLERASG
                     IRVEVISGDEEARLITLAVGKRIPLGKKSALIVDLGGGSVEITFVENGRITLADSHNF
                     GALRLLDMLSSAEEDLRSAGLLLGEYMELVGRKLSRRGNGKKAALFIATGGNIEAIAS
                     IPDVRAEPHPDYPETVRIKAGNLRRLMEELSGMSLKSRMERFGLREDRADVILPACFV
                     YHRVAELNGSEEILVPRVSLKDGVIQEILEQAKGAEHLLDLREQVCVSCREMVARYKL
                     DQRHTEKVTELALSLFDQTEPLHGLGKQERIYLEAGALLHDVGYFISMQKHHKHSQYI
                     IANSEIVGLTQAERLIVAGISRYHRKATPDPAHPEYEALSKKDRATVSSLAAILRIAD
                     ALDKEHNSAIRSVECRLQDGSLLLRAVSRKSCRLEALGVTANAAMFRDHFGVDVKLTI
                     EQEG"
     gene            1928..2629
                     /gene="tmk"
                     /locus_tag="VF357_00350"
     CDS             1928..2629
                     /gene="tmk"
                     /locus_tag="VF357_00350"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7522622.1"
                     /translation="MPEKTIPNTTPGKLIAVEGLDGSGKSTQVYLIKRWLELSGYKVF
                     FTEWNSSEIVRSATRRGKKQNLLTPTTFSLIHCTDFADRYERNILPMLKAGFIVLADR
                     YKFTALARDTVRGCPQEWVEQLYSFAFQPDITFYFSLPLRTALDRILVGRPALKYHEA
                     GMDLGLSPDPVESFKIFQGRILKAYETLARRHRFTRIDSDRPVEAIQEEVRAILRDRI
                     DLAQYKVAVPRGWRL"
     gene            2626..3324
                     /locus_tag="VF357_00355"
     CDS             2626..3324
                     /locus_tag="VF357_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522623.1"
                     /translation="MNQPRTFYGKGIPGVATGDLKGKLVVLEGPDGSGRSTQIAFLTE
                     WLERRGHAVEHVGLKRSNLVSSELAAAKEGNVLSPRTLSLFYATDFADQLENRILPAL
                     RAGFIVLADRYIYTLMARDIVRGADLAWLQSVYSIALVPDLVMYLRVAPQKLMERYIY
                     RGRELDYWESGMDIGYSRSWYDSFIRYQSELHRTFNKLQAIYGFATINGMRVPRAITR
                     DLQTRIEAVISPSG"
     gene            3337..3696
                     /locus_tag="VF357_00360"
     CDS             3337..3696
                     /locus_tag="VF357_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522624.1"
                     /translation="MATAKGKAAAGKAAPAGKKSIAAALARKPSRKVAKDMLVLELRR
                     LRAMFTEIAERYVANAEGEIAAVIEAVEGKSLSAARIEKMLEVVRALSVKPRKGRRKD
                     LTRIETIAASLRKELGE"
     gene            3707..4552
                     /locus_tag="VF357_00365"
     CDS             3707..4552
                     /locus_tag="VF357_00365"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX7522625.1"
                     /translation="MEAAAFLAESGRTRSARFRECLKRAAANPASDGAVHDLRVAIRR
                     ILSWIAVRDALLGPDRRLLKARISLKALMSPLGKLRDAHVKRDWIRSVLPEGDEPSYQ
                     YSVQVASDVLRWEAGVRKRLGAKSTGRLRLPLPKGVGDRGGRIEAATAAPEILGELER
                     EVSKHRKAALDPAHPEALHRMRLAFKKYRYAAELLLPLFPKATEETAKRLHAFQSLLG
                     TIHDCDMILAEAASFRRDILGRKKESALEIAFRAFREKSFGEFRRTAGTTGRLSRLFG
                     TAFRS"
     gene            complement(4658..5140)
                     /locus_tag="VF357_00370"
     CDS             complement(4658..5140)
                     /locus_tag="VF357_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522626.1"
                     /translation="MLSARLIELIQAHAEHLTRDVLKDYATNPRTRHWNVVPGAELEQ
                     RVISTYRNLGNWIGDHREDAVQAEYEEWGRKRYRNGIPLSEVVFAVILLKQHLRKYIR
                     EHGLVEHSRDRQSPLEILPVHLYGIQELNYLVGDFFDKALYHLARGYEMEAQAAQGTR
                     "
     gene            5350..>6333
                     /locus_tag="VF357_00375"
     CDS             5350..>6333
                     /locus_tag="VF357_00375"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7522627.1"
                     /translation="MRYLILGSGPAGIAAAKAARKLDKDAEVLLATEEHAAPYLRPLL
                     PDIVSGERELSGVADPEGKDLAGYEVKLLGGKRARRIDAAKNRMTFSDGSEETYNFLC
                     VATGGRPILPLALMGAPGSFLFLNSLGDAQRVRERAMRSDTTVVYGPGYLGIEAARAM
                     RKLGNEVIWINPGLPRFGNPISGDVETRVTALLRARGVKIHEGTEIADVIDVDGKNFE
                     VMTAGGGTIRCHLIVVATERLPNIGFLEGSGVKAGAGILVDEYLRTNVSNIYAAGDCA
                     EVYDINRRESRINFGWRSAIKQGQLAGENMAGGGKVYIRNAEDYFGLLYGTP"
BASE COUNT         1165 a         2082 c         2069 g         1017 t
ORIGIN      
        1 cgtcgagcgg gcggagccgt tgctgaccgt ccccccgctc aagccgctga tcgacatccc
       61 caggatggcc gacatcgcgc aggagatgct caaggcctcc ctggacgcct tcgtcaacgg
      121 ggacgacgaa ttggcgttcg ctacgatcct tagggacgac ctggtcgacc agctcaagga
      181 ccaggtgttc cgcgagctgc tgaccttcat gatggcggac ccgaccacca tcccccgggc
      241 gatggacctg atcctcgtct cccggcacct cgagcggatc gcggaccacg ccaccaacat
      301 ctgcgaggac gtgatcttca tggtcaaggg gaaggacgtc cggcaccagg ggccgctggc
      361 gtaggaggga aaggaaaccg cgaatggcac gcatcgccgc catcgacatg gggtccaacg
      421 cgatccgttt ctacgtggtg gagacggggg gggactcctc gtaccgcgtc ctggagaacg
      481 tccgggaacc gatccgcctg gggggagacg tcttcctgaa ggggaccatc cgggaagaga
      541 acatccggaa ggccgaggcc gccttccggc gcttccgcca gctcctgaag gcccacgggg
      601 tccagacggt acgtgccgtc gccacctgcg ccacccggga ggcgtccaac gccgacctcc
      661 tgctcagccg cctcgagcgc gcctccggaa tccgcgtgga ggtgatcagc ggcgacgagg
      721 aagcccggct gatcaccctc gccgtcggga agaggatccc cctcgggaag aagagcgccc
      781 tcatcgtcga cctcggcggc ggctcggtgg agatcacctt cgtcgagaac ggccggatca
      841 ccctggccga ctcccacaac ttcggtgcgt tgcgcctgct ggacatgctc tcctccgcgg
      901 aggaggacct ccggagcgcg gggctgctgc tgggcgagta catggagctg gtgggccgga
      961 agctgtcgag gcgcggaaac gggaaaaagg ccgccctgtt catcgccacg gggggaaaca
     1021 tcgaggccat cgcttcgatc cccgacgtgc gcgcggagcc gcaccccgac tacccggaaa
     1081 ccgtgcgcat caaggcgggg aacctccgtc ggctcatgga agagctctcc ggcatgtcgc
     1141 tgaagtcccg gatggagcgg ttcgggctgc gggaagatcg ggccgatgtc atcctccccg
     1201 cttgcttcgt ataccaccgg gtcgcggagc tgaacggctc cgaggagatc ctcgtccccc
     1261 gggtgagcct gaaagacggc gtgatccagg agatcctcga gcaggcaaag ggagccgaac
     1321 acctcctgga cctccgggag caggtttgcg tgtcgtgccg ggagatggtc gcgcggtaca
     1381 agctggacca gaggcacacg gagaaggtca ccgagctggc cctcagcctg ttcgaccaga
     1441 cggaaccgct ccacggcctc gggaagcagg agcggatcta cctcgaggcg ggtgcgctcc
     1501 tgcacgacgt cgggtatttc atcagcatgc agaagcacca caagcacagc cagtacatca
     1561 tcgccaactc cgaaatcgtc gggcttaccc aggcggagcg gctcatcgtc gccgggatct
     1621 cgcgctacca ccggaaggcc acgccggacc cggctcaccc ggagtacgag gcgctttcca
     1681 agaaggaccg ggccaccgtg tcgtccctcg ccgctatcct tcgaatcgcc gacgcactgg
     1741 acaaggagca caactccgct atccggagcg tcgaatgccg cctgcaggac gggtcgctgc
     1801 tcctgcgggc ggtgagccgg aagtcgtgcc gcctggaggc gttgggcgtc accgccaacg
     1861 cggcgatgtt ccgcgaccac ttcggcgtgg acgtgaagct gaccatcgag caggaagggt
     1921 gaccgacatg ccggaaaaga ccatcccgaa caccaccccc ggaaaactga tcgccgtcga
     1981 ggggctcgac ggatccggga agtcgaccca ggtgtacctc attaaacggt ggctggagct
     2041 atccgggtac aaagtcttct tcaccgagtg gaactcctcc gagatcgtcc ggagcgccac
     2101 ccggcgggga aagaaacaga acctgctcac cccgaccacc ttctccctca tccattgcac
     2161 cgacttcgcc gaccggtacg agcggaacat ccttccgatg ctcaaggcgg ggttcatcgt
     2221 cctcgcggac cggtacaagt tcaccgccct cgcacgcgac accgtccggg ggtgcccgca
     2281 ggagtgggtc gaacagctct acagcttcgc gttccagccg gacatcacct tctacttctc
     2341 cctcccgctg cgaacggcgc tggaccggat cctcgtcggt cgtccggcgc tcaaatacca
     2401 cgaggcggga atggacctcg gcctctcccc ggatcccgtg gagagcttca agatcttcca
     2461 ggggaggatc ctcaaggcgt acgaaaccct cgcgcgccgg catcggttca cccggatcga
     2521 ctcggatcgt cccgtggagg cgatccagga ggaagtccgg gcgatcctgc gcgaccggat
     2581 cgacctcgcg caatacaagg tggcggtccc gagggggtgg cggttatgaa ccaaccaagg
     2641 acgttctacg gcaaggggat tccgggagtc gccaccggag acctcaaagg gaagctcgtc
     2701 gtgctcgaag ggccggacgg gtccgggcga agcacgcaga tcgcctttct caccgagtgg
     2761 ctggagcggc gcgggcacgc ggtcgagcac gtcgggctga agcggtccaa cctggtctcc
     2821 agcgagctcg ccgcggccaa ggaaggaaac gttctctccc cccgaaccct gtcgcttttc
     2881 tacgccaccg acttcgccga ccagttggaa aaccggatcc tcccggccct gcgggcgggg
     2941 ttcatcgtcc tcgccgaccg gtacatctat accctgatgg cgcgtgacat cgtacgggga
     3001 gccgaccttg cctggctgca atcggtgtac agcatcgccc tcgtccccga cctggtgatg
     3061 tacctccggg tggctcccca gaagctcatg gaacggtaca tctaccgcgg aagggagctc
     3121 gactactggg agtcggggat ggacatcggg tacagccggt cctggtacga ctcgttcatc
     3181 cgctaccagt cggagcttca caggaccttc aacaagctgc aggccattta cggattcgcg
     3241 accatcaacg gcatgagggt ccctcgcgcc atcacgcgcg acctgcagac ccggatcgag
     3301 gccgtcatct cgccgagcgg atagggagga aatcgtatgg ccaccgccaa agggaaagcg
     3361 gccgcgggga aagcggcccc ggcagggaaa aagagcatcg cggcggcatt ggcgcgcaag
     3421 ccttcccgga aggtggcgaa ggacatgctc gtcctcgagt tgcgccgcct gcgggcgatg
     3481 ttcacggaga tcgcggaacg gtacgtcgcc aacgcggaag gagagatcgc ggccgtgatc
     3541 gaggccgtcg aagggaaatc cctctccgcc gcccgcatcg aaaaaatgct ggaggtcgtt
     3601 cgcgccctct ccgtaaagcc ccggaagggg cggcggaagg atctgacgcg catcgagacc
     3661 atcgccgctt ccctccgcaa ggagcttggg gagtaagggg accccgttgg aggcggccgc
     3721 cttcctcgcg gagtcgggac ggacgcgttc ggcccgtttc cgggagtgcc tgaagcgcgc
     3781 tgccgcaaac ccggcgtcgg acggggccgt ccacgacctg agagtggcga tccgtaggat
     3841 tctctcctgg atcgccgtgc gcgacgccct gctgggcccc gaccggcggc tgctcaaggc
     3901 ccgcatctcc ctcaaggcgc tcatgtcccc gctggggaag ttgcgcgacg cccacgtgaa
     3961 gcgggactgg atccggagcg tgctcccgga aggggacgaa ccgtcctacc aatactccgt
     4021 gcaggtcgcg agcgatgttc tccgatggga ggccggggtc cggaagcgcc tcggggcgaa
     4081 atccaccggg cggcttcgac tcccccttcc caagggggtt ggagaccgcg gggggcggat
     4141 cgaagcggcg accgccgccc cggagattct cggggaactg gaacgcgagg tctcgaagca
     4201 ccgaaaggcg gccctcgacc cggcgcaccc ggaagcgttg caccggatgc ggctcgcctt
     4261 caagaaatac cgctacgccg cggagctcct cctcccgcta tttccgaagg cgacggagga
     4321 aacggcaaag cgcctccacg ccttccagag cctcctcggg acgatccacg attgcgacat
     4381 gatcctcgcc gaagccgcgt cgttccgccg cgacatcctc ggccggaaga aggaatccgc
     4441 gttggagatc gctttccgcg cgttccggga gaagagtttc ggggaattcc ggcggaccgc
     4501 ggggacgacc gggaggcttt cacgactgtt cgggacagcg ttccgatcct gaaaccggat
     4561 tttcttttct ggtggaaacg atcggccggg tgccgtttcc cgcgctatcg cggccgttca
     4621 tgcagaccga gatcttctgt cccaacgcga tccgtatcta ccgggtgcct tgcgccgcct
     4681 gggcttccat ctcgtagccc ctcgccaggt ggtagagcgc cttgtcgaag aaatccccca
     4741 ccaggtaatt cagttcctgg atcccgtaca gatggacggg aaggatctcc agcggggatt
     4801 ggcggtcccg ggagtgctct acgagcccgt gctcgcggat gtatttccgc aggtgctgct
     4861 tgagcaggat cacggcgaac acgacctcgc tcagcgggat cccgttccgg taccgcttgc
     4921 ggccccactc ctcgtactcc gcctgcaccg cgtcttccct gtgatcgccg atccagttcc
     4981 caaggtttct gtaggtgctg ataacgcgct gttcgagctc cgcgccgggc acgacgttcc
     5041 agtggcgcgt ccgcgggttg gtggcgtaat ccttcagcac atcgcgtgtc aggtgctcgg
     5101 cgtgagcctg gatcagttcg ataaggcgcg cagagagcaa ggcgttcgac ctccacgagg
     5161 gaatttcctt ccgtcgccca acctgtggct ggacgaccag gagctttccc cggcattgta
     5221 cccggcccgc aagggtatgc aagcaggatt ccccggcagg aaccgtggac ctgaaactct
     5281 ctttccgttt tgcacctcct actctcaaat ggtagattcg gtatcgaaat tcgactacgg
     5341 agggatccca tgagatacct gatcctcgga agcggcccgg ccggaatcgc cgccgcgaaa
     5401 gccgcacgca agctcgacaa ggatgcggag gtcctcctcg ccaccgagga gcacgccgcg
     5461 ccgtaccttc gtcccctgct ccccgacatc gtttccggcg agcgggaact gtcgggcgtc
     5521 gccgacccgg agggaaagga cctcgcggga tacgaggtga agctcctcgg cggaaagcgc
     5581 gcgcgccgca tcgacgcggc gaagaaccgt atgacctttt ccgacggctc cgaggaaacg
     5641 tacaatttcc tgtgcgtcgc cactgggggc cgaccgatcc tgcccctcgc gctgatgggg
     5701 gcgccgggct ctttcctctt cctcaattcc ctcggggacg cgcagcgggt ccgcgagcgg
     5761 gcgatgcgct ccgacacgac ggtcgtctac ggccccggct acctcgggat cgaggcggcg
     5821 cgggcgatgc gcaagctggg gaacgaggtg atctggatca atccggggct tccccggttc
     5881 gggaatccca tctcgggaga cgtcgagact cgggtcaccg ccctgctccg ggcccggggc
     5941 gtcaagatcc acgaagggac ggagatcgcc gacgtgatcg acgtcgacgg gaagaacttc
     6001 gaggtgatga cggcgggggg cggaacgatc cggtgccacc tgatcgtcgt cgccaccgag
     6061 cgactcccca acatcggctt cctcgagggg agcggcgtga aggcgggcgc gggcatcctg
     6121 gtcgacgagt acctgcgtac caacgtcagc aacatctacg cggcgggcga ctgcgccgag
     6181 gtgtacgaca ttaaccgccg ggagagccgg atcaacttcg ggtggcgcag cgcgatcaag
     6241 cagggacagc tcgccggcga gaacatggcc ggcggcggca aggtctacat caggaacgcc
     6301 gaggattact tcgggcttct gtacgggacc ccg
//
LOCUS       DASVJL010000019         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1034715, whole genome shotgun sequence.
ACCESSION   DASVJL010000019 DASVJL010000000
VERSION     DASVJL010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034715"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..2187)
                     /gene="mnmG"
                     /locus_tag="VF357_00380"
     CDS             complement(307..2187)
                     /gene="mnmG"
                     /locus_tag="VF357_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX7522628.1"
                     /translation="MSGSPYEYPKEYDVIVVGGGHAGCEAALAAARMGCSTLLVTINA
                     DAIGLMSCNPAIGGLAKGHLVREIDALGGEMGRNIDATGIQFRQLNTSKGPAVRSSRA
                     QADKQLYRLRMKKALEATYGLDVKQAIVDAVVVDGGAAVGVDTNLDVRYRGKTVVLCP
                     GTFMKGLVHVGLVSYPAGRAGDFPSMRLSDSLRRLGFSVGRLKTGTTPRVDAKTIDFS
                     KTVRQEGDDRPVPFSFDTAAIRRPQLPCFMTYTNAATHEAIRSGLSRSPLYSGIIKGI
                     GPRYCPSIEDKVVRFSGKERHQVFLEPEGLKTAEYYPNGVSTSLPYDIQVKMLRTIPG
                     LESVEIVRPGYAIEYDFVDPVQLHPSLETKLVKNLFHAGQINGTSGYEEAGAQGIVAG
                     INAALRVNGEPPMVLSRADAYIGVLIDDLTTKGAREPYRMFTSRAEYRLLLREDNADL
                     RLSETGRRVGLLPEERYKRFLAKREGIGVFRKRLEETRVGAGHPLCAAVEKAGDSLLR
                     PGITYAEALRRPGVTGAMLLACDPELRVATREAVEQAELAIKYEGYIRRQEEEAKNLK
                     KYEEMRFPSAFPFETVPGLSAEVRDKLVRVRPDSIGQAQRIPGVTPAAVALLLVVLRR
                     GA"
     gene            complement(2243..>3062)
                     /locus_tag="VF357_00385"
     CDS             complement(2243..>3062)
                     /locus_tag="VF357_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX7522629.1"
                     /translation="PAITSRQLIERVSEIMERLEELLRSYEAGHRFRDGATVVIAGVA
                     NVGKSRLLNRLAGEERAIVTETPGTTRDYLHADVPIGGVPVTVIDTAGLRETADPVER
                     EGVRRSREIVASADLVLFVLDGSRAASTGDRSAYEEVASLPHLLLLNKSDLAAAEEGS
                     GFAGAGKRGAMRLSAKTGEGIGDLVAAVAREVAPAEGAIRAQAPLTRARQRLAVERAM
                     SALSRAKAAVEEGLPLEFPAADVREAAGALAELLGEVAPEEVLDAIFGAFCIGK"
BASE COUNT          487 a         1131 c          925 g          519 t
ORIGIN      
        1 ccgcggcact tgcccgtcca tgggcatcgg ggttccccac gcagtgcccc ccgtttttat
       61 tacagagggg gtaccccggc agcgcgaagc tcgtatcggg gcggcgtctg gagcgcagcg
      121 gagacaggga cggcgggagc gagcggaagc gcaggcgagg agaccatgga cgggcgaccg
      181 ctcccgccat ccatggctcc cgcggcactt gcccgtccat gggcatcggc gatggtagcg
      241 gagtggaagc ccccgcgagc ccgtagtccc ggtttgatgc gttaccgtat atataaactg
      301 atgcacttag gcgccccgcc ggagcacgac gagcaggagg gcgacggctg ccggggtcac
      361 cccgggaatt cgctgcgcct ggccgatgga gtcgggccgg acccggacga gcttgtcccg
      421 gacttcggcg gacaggccgg gaacggtttc gaacgggaac gcggatggga aacgcatctc
      481 ctcgtatttc ttaagatttt ttgcctcctc ttcctgccgc cgaatatacc cttcatactt
      541 gatggccagc tccgcctgct ccaccgcctc ccgggtcgcg acccgcagtt ccgggtcgca
      601 ggcaaggagc atcgcgcccg tcaccccggg ccgccgcaac gcctccgcgt aggtgatgcc
      661 gggccgcagg agcgaatccc ctgccttctc caccgcggcg cacagcgggt gtccggcacc
      721 cacgcgggtt tcctcgagcc gcttgcggaa caccccgatc ccctcccgct tcgcgaggaa
      781 tcgcttatac cgttcctccg ggagcagccc gacccgtcgc cccgtttcgg acaaacgcag
      841 atcggcgttg tcctcccgca gcagcagccg atactccgcc cgggaggtga acatccggta
      901 cggctcccgc gcccccttcg tcgtcaggtc gtcgatcaag accccgatgt aggcgtcggc
      961 acgcgagagg accatcggcg gctcaccgtt cacccgaagc gccgcgttga tccccgccac
     1021 gatcccttgc gctcccgcct cttcgtaccc gctcgtgccg ttgatctgcc cggcatgaaa
     1081 gaggtttttc accagtttcg tttcaaggga ggggtgcagc tgcaccggat cgacgaagtc
     1141 gtactcgatc gcgtacccgg gtcggacgat ctccacggat tccaaccccg ggatggtgcg
     1201 gagcattttc acctggatgt cgtacggcaa gctcgtggag accccgttgg gataatactc
     1261 cgcggttttc agcccctccg gctcgaggaa gacctggtgc cgctccttcc cggagaaccg
     1321 aacgaccttg tcctcgatgg aggggcaata ccgggggccg atccccttga tgattccgga
     1381 atacaggggc gaccgggaaa ggccggaccg gatcgcctcg tgggtcgcgg cgttcgtgta
     1441 cgtcatgaag cacggaagct gcggccgacg gattgccgcc gtgtcgaacg aaaaggggac
     1501 cggccggtcg tccccctcct ggcggacggt cttcgagaag tcgatcgtct tggcgtcgac
     1561 ccggggggtg gtgccggttt tcaggcgccc gacggagaac ccgagccgac gcaacgaatc
     1621 cgacaggcgc atcgacggga aatcccccgc ccttcctgcc gggtagctca ccagccccac
     1681 atggaccaat cctttcatga aggtcccggg acaaaggact acggtctttc cgcggtaccg
     1741 gacgtccagg ttcgtgtcca cccccaccgc cgcgcccccg tccaccacga ccgcgtcgac
     1801 gatcgcctgc ttcacgtcca gcccataggt ggcctccagc gccttcttca tccggagacg
     1861 gtagagctgc ttgtccgcct gggcgcgcga ggagcggacc gccggcccct tgctcgtgtt
     1921 cagctggcgg aactggattc cggtggcgtc gatgttccgc cccatctccc cgcccaacgc
     1981 gtcgatctcc cgcacgaggt gtcccttcgc caatcccccg atcgccgggt tgcacgacat
     2041 gaggccgatg gcgtccgcat tgatcgtcac caggagggtc gagcacccca ttcgcgccgc
     2101 cgccagcgcc gcctcgcagc ccgcgtggcc gcccccgacg acgatgacgt cgtactcctt
     2161 cgggtattcg tacggagacc cgctcatcgc gccccgctca atgttccacg ggaaacattc
     2221 tatatattct atttattatc aactacttgc ctatacagaa cgcgccaaag atcgcgtcga
     2281 ggacctcttc cggggcgacc tcccccagca actccgcgag ggccccggcc gcctcccgga
     2341 cgtccgccgc cgggaactcg agcgggagtc cctcctccac cgccgccttc gcgcgggaga
     2401 gggccgacat cgcccgctcg accgccaacc gctgccgcgc gcgggtcagc ggcgcctgcg
     2461 cccggatcgc accctccgcc ggcgcaacct cccgggcgac cgccgccacg agatccccga
     2521 tcccctcccc cgttttcgcg gagaggcgca tggctcccct ctttcccgct ccggcgaatc
     2581 cgctcccctc ctccgccgcc gccaggtccg acttgttcag gaggagcaga tgcggaagcg
     2641 aagcgacctc ttcgtacgcc gaacggtctc ccgtcgacgc cgcccgactc ccgtccagaa
     2701 cgaagaggac gagatccgcc gacgcgacga tctcccggct tcgccgcacc ccttcccgct
     2761 ctaccgggtc cgccgtctcc cgaagccccg cagtgtcgat cacggtcacc gggacgccgc
     2821 cgatgggaac gtcggcgtgg agataatccc gcgtggtccc cggcgtctcc gtcacgatcg
     2881 cgcgctcctc ccccgccagg cggttgagca gccgcgattt cccgacgttc gcgacaccgg
     2941 cgatcacgac cgtcgcgccg tcccgaaacc ggtgccctgc ctcgtacgac cgcaggagct
     3001 cctccagccg ctccattatt tccgacaccc gttctataag ttgtcggctt gtgatcgcgg
     3061 gg
//
LOCUS       DASVJL010000020         1685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_96947, whole genome shotgun sequence.
ACCESSION   DASVJL010000020 DASVJL010000000
VERSION     DASVJL010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96947"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..774)
                     /locus_tag="VF357_00390"
     CDS             complement(82..774)
                     /locus_tag="VF357_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX7522630.1"
                     /translation="MQTRPAPTSAITLEKASFFPRVYGWMALGLLVSAVASFALLASP
                     AAMKFVFGTKWVFFGLILGELGLVFYLSSRVMSMSSAAAKGTFLVFAGLNGVTLASIF
                     LVYTAGSIASTFMVSAATFGAMSAYGMATKRDLTGFGSFVVMGLFGIVIASVVNLFLK
                     SEAVYWVTTYIGVLVFVGLSAYDTWKLKRMAETSGYGEALANLSILGALTLYLDFVNL
                     FLMLLRLLGRRK"
     gene            complement(886..1392)
                     /locus_tag="VF357_00395"
     CDS             complement(886..1392)
                     /locus_tag="VF357_00395"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HEX7522631.1"
                     /translation="MLTPRFEPASRKNVDRTRDPYIPRKGPLDVGVCPECHAISRKKR
                     WYLDEAEYVSLARSGAVLRRCPACRKIADGFPSGVVTLRGRFLQTHRDEILTIVRNEE
                     RRARETNPLERIMDIRDGGESVEILTTDEKLAQRIGREIRKAYQGTASYKWSEDANLL
                     RVNWARDI"
     gene            complement(1469..>1685)
                     /locus_tag="VF357_00400"
     CDS             complement(1469..>1685)
                     /locus_tag="VF357_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522632.1"
                     /translation="STEKPVEYIFLVLTPAESPDAQVRILGILARVSRNRHLLLKIQS
                     CRTPEEVLSAVQDWEAQQAVSGIEPAP"
BASE COUNT          324 a          555 c          509 g          297 t
ORIGIN      
        1 cgtagaccgc gacaaagcct cggaacttct tggtgaggcg gtgcgtttcc aatatgtagt
       61 cgccgcctga cacccccccc actactttct ccttcccagc aaccggagga gcatcaggaa
      121 gaggttgacg aaatccaggt ataaggtcag cgccccgagg atcgaaaggt tggcgagcgc
      181 ttcgccgtac cccgaggtct ccgccatccg cttcagcttc caggtgtcgt aggccgaaag
      241 cccgacgaag accaggaccc cgatgtaggt ggtgacccag tagaccgcct cgcttttaag
      301 aaaaaggttc accaccgacg cgatgacgat cccgaagagg cccatgacga cgaagctgcc
      361 gaaccccgtc aagtcccgtt tcgtcgccat gccgtacgcg ctcatcgccc cgaacgtcgc
      421 cgccgacacc atgaacgtgg acgcgatcga ccccgccgtg tagaccagga agatggaggc
      481 gagggtgacg ccgttcagcc ccgcgaacac caggaacgtc cccttcgccg ccgccgacga
      541 catcgacatc acccgcgagc tcaggtagaa gaccagcccg agctccccga ggatgaggcc
      601 gaagaagacc catttcgtcc cgaagacgaa cttcatcgcc gcaggggacg cgaggagcgc
      661 gaacgatgcg acggccgata cgagcagccc gagcgccatc cacccgtaca ccctcgggaa
      721 gaaggaagcc ttttccaacg tgatcgccga cgtcggggcc ggccttgtct gcatgttgaa
      781 atccattaca ccctcctccg tgaaacgtgc gatttttcca gcgtcattct cgcacaatcg
      841 cggagatgac gtttgcgcgg gaaccgggct cccgaggttt cctcgtcaga tgtcccgtgc
      901 ccagttcacc cggagaagat tggcgtcctc ggaccacttg taggacgccg tcccttggta
      961 cgccttccgg atctcccgcc cgatgcgctg ggcgagtttc tcgtccgtcg tcagaatctc
     1021 cacgctctcg cccccgtccc ggatatccat gatccgctcg agaggattcg tctcacgggc
     1081 ccggcgttcc tcgttacgga cgatcgtgag gatctcgtcc ctgtgggtct gcaggaacct
     1141 cccgcgcagg gtcaccacgc cggaaggaaa tccgtccgcg atcttccggc acgccgggca
     1201 ccggcggagg accgcgccgg atcgcgcgag ggagacgtac tccgcctcgt ccaggtacca
     1261 acgcttcttc cggctgatgg cgtggcactc cgggcagact ccgacgtcga gggggccttt
     1321 ccgcgggatg taggggtctc tcgtgcggtc gacgttcttg cggctggcgg gttcgaaccg
     1381 gggcgtcaac atggtttcac cctccctgga gcggctggaa agggttggac gcgctgaaac
     1441 cttccatttc catgataccg cagaaagctt acggagcggg ttcgatcccg ctgacggcct
     1501 gctgcgcctc ccaatcctga acggccgaaa gaacctcttc cggggtacgg caagattgga
     1561 tcttgaggag gaggtgccgg ttccgggaga cccgcgcaag gatccccagg atccggacct
     1621 gggcgtcggg agactccgcg ggcgtgagca ccaggaagat gtactccacc ggtttttcgg
     1681 tggag
//
LOCUS       DASVJL010000021         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1409880, whole genome shotgun sequence.
ACCESSION   DASVJL010000021 DASVJL010000000
VERSION     DASVJL010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409880"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VF357_00405"
     CDS             complement(<1..829)
                     /locus_tag="VF357_00405"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX7522633.1"
                     /translation="MSSTVWFTDLSARPGNSLIDKTGMLLRAAGIQERPGRGALTAVK
                     LHFGEKGNTAFIRPIFVRKVVEEIAAAGGRPFLTDTNTLYVGTRSNAVDHLNTAITNG
                     FAWAVAGAPLVVADGLRGERAVRGPIAGKIFQEVAIGAEIAHADAMVVVTHFKGHELT
                     GFGGTLKNLGMGCASRSGKLSEHSSVSPAVDPSGCTGCGTCVAHCPSGAIAVVASKAA
                     ISPDACIGCADCIVVCPEGTVTVNWNEASSTVQQKMVEHALGAVSGKRSSSLFLSFVT
                     "
     gene            926..1414
                     /locus_tag="VF357_00410"
     CDS             926..1414
                     /locus_tag="VF357_00410"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX7522634.1"
                     /translation="MDFKAGDMAVYPAHGVGIVEKIETKCFNDGKRESFYVLRILDTG
                     ITIMIPMGNAEQVGLRAIMDASAVRSVYKILKEREVELEPKPWNRRYRMYMEKLKSGS
                     PFDIAEVLRNLLLLKSGKALSFGERKMLDSARSLLVKEISIAKSVTEEVVETDLRRFL
                     NL"
     gene            1503..>1935
                     /locus_tag="VF357_00415"
     CDS             1503..>1935
                     /locus_tag="VF357_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX7522635.1"
                     /translation="MIGTVVIVVAGGTGRRMGAVVPKQFLSVGGRSLLDRTLSSVSAA
                     SRVDAIVLALPADAAPEAGEAYRGYPKVIAVVKGGAERHDSVRNALAAVPSVATIILV
                     HDAVRPFATPGLFDRCAEEALRHGAAVPVIPVRDTVKTWDKA"
BASE COUNT          336 a          631 c          632 g          336 t
ORIGIN      
        1 gggtgacgaa ggagaggaac agggaagacg accgcttccc cgagacggct ccgagcgcgt
       61 gctcgaccat cttttgctgg accgtcgagg acgcctcgtt ccagttcacg gtcaccgtgc
      121 cctcggggca gacgacgatg cagtccgcgc atccgatgca cgcatccgga gagatcgccg
      181 ccttcgacgc gacgacggcg atcgctcccg acgggcagtg ggcgacgcag gtcccgcacc
      241 cggtgcaccc tgagggatcg acggcgggcg acaccgaaga gtgctcggaa agttttccgg
      301 agcgcgacgc gcaacccatt ccgaggtttt tcagagtgcc cccgaatccg gtgagttcgt
      361 gccccttgaa atgggtcacc acgaccatcg catccgcatg ggcgatctcg gccccgatcg
      421 ccacctcctg gaagatcttc cccgcgatcg gcccccggac ggcgcgttcc ccgcggagac
      481 cgtccgccac gacgaggggc gcgcccgcca cggcccaggc gaaaccgttc gtgatcgcgg
      541 tgttcaggtg atcgaccgcg ttgcttcggg tgccgacgta aagtgtgttc gtgtcggtca
      601 gaaacggcct ccccccggcg gcggcgatct cctcgacgac cttgcggacg aagatcgggc
      661 ggatgaaggc ggtgttcccc ttctccccga agtggagctt gacggcggtc agggcgcccc
      721 gtccgggccg ctcctggatt ccggccgctc gcaggagcat ccccgtcttg tcgatcaggc
      781 tgttccccgg acgcgcggaa agatccgtga accagacggt cgatgacatc gattctcgcc
      841 tcgcgttgac gttaatgaga aaatgcccta taatacactg aaaaaaggga tcctgatcgc
      901 tgggatgaat cctctggagg tccgcatgga tttcaaggca ggggatatgg cagtataccc
      961 cgctcacggg gtcggcatcg tcgaaaagat cgagacgaag tgcttcaacg acggcaagag
     1021 ggaatcgttc tatgtcctga ggatcctcga cacggggatc accatcatga tcccgatggg
     1081 caacgcggaa caggtcgggc ttcgggcgat catggacgcc tcggcggtcc gctccgtcta
     1141 caagatcctg aaggaacgcg aggtggagct cgagccgaag ccgtggaacc ggcggtaccg
     1201 gatgtacatg gaaaagctca agtccggctc tcccttcgac atcgccgagg tgctgcgcaa
     1261 cctgctgctc ctgaagtccg ggaaggccct ctccttcggc gaaaggaaga tgctggactc
     1321 ggcgcgttcc ctgctcgtga aggagatctc gatcgcgaaa tccgtcacgg aggaagtcgt
     1381 tgagacggac cttcgccggt tcctgaacct gtagggcaac gggcatcttc gcggtttctc
     1441 actaactgtc gcggccccga ttccccggga tcggtcggtt cccgatccat tccggaggtg
     1501 tgttgatcgg tacggtggtc atcgtcgtcg ctggcggaac ggggcgccgg atgggcgccg
     1561 tcgtgcccaa gcagtttctc tccgttggcg gccggtctct cctggaccgg acgctctcct
     1621 cggtttccgc cgcctcccgc gtcgacgcga tcgtcctcgc cctccccgcc gacgccgctc
     1681 ccgaagcggg cgaggcgtac cggggatacc cgaaggtgat cgcggtggtc aagggggggg
     1741 ccgagcggca cgactcggtc cgcaacgccc tcgccgccgt gccttcggta gccacgatca
     1801 tcctcgtgca cgacgcggtc cgccccttcg cgacccccgg cctcttcgac cggtgcgcgg
     1861 aagaggccct ccggcacgga gcggcggtcc cggtgatccc cgtccgagac acggtgaaga
     1921 cgtgggacaa ggcgg
//
LOCUS       DASVJL010000022         1842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_190985, whole genome shotgun sequence.
ACCESSION   DASVJL010000022 DASVJL010000000
VERSION     DASVJL010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190985"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /gene="aspS"
                     /locus_tag="VF357_00420"
     CDS             <1..1233
                     /gene="aspS"
                     /locus_tag="VF357_00420"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX7522636.1"
                     /translation="YLVPSRVNPGMFYALPQSPQLFKQILMIAGYDRYAQIVKCFRDE
                     DLRADRQPEFTQIDVEMSFIDRDDIFLMMEGLMARVFHDVLGEEIRSPIPRMTYREAM
                     DRFGVDKPDTRFGLEITDHTALLSKTEFRVFAEVASRGGVIRGITAPGMGESSRSEIA
                     ALEEVAKIGGASGLASFKVTEAGLTSSLAKFFRAEQMASLREAAGAQPGDLILLVADS
                     FDVACSSLGRLRLHLGEKLGMIDASLHHLLWVTDFPLLEWDKEEKRWGAMHHPFTAPL
                     DEDLPLLATDPGKVRAKAYDMVLNGSEIGGGSIRIHRKDVQSRMFDLLNIGPEDARVK
                     FGFLLEALEFGAPPHGGIAFGLDRLAMLFSGAKSLRDVIAFPKTQKATCLMTEAPSGV
                     DLKQLRELSIRVTVPEKK"
     gene            1253..>1842
                     /locus_tag="VF357_00425"
     CDS             1253..>1842
                     /locus_tag="VF357_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX7522637.1"
                     /translation="MLPGELFHPGSVAVVGASRNAEKVGYAVFANLVQAGFRGKIYGV
                     NPGGGELFGHPLYPSIDAIPGPVDLGVFVVPPNAILEGIPRLAAKGMRAGIVISAGFK
                     EIGGAGVALERSLREVATSAGVRVLGPNCLGLIDTHSSLNVSFSRGTPPKGFISFFSQ
                     SGALATAVLDWAIGRNIGFSKFVSLGNKVDISESDIL"
BASE COUNT          293 a          599 c          628 g          322 t
ORIGIN      
        1 tacctggtgc cgtcccgcgt caatccgggg atgttctacg cccttcccca gtccccgcaa
       61 ctgttcaagc agatcctgat gatcgccggg tacgaccggt atgcgcagat cgtcaagtgc
      121 ttccgggacg aggacctgcg cgccgaccgg cagcccgagt tcacgcagat cgacgtcgag
      181 atgtcgttca tcgaccggga cgacatcttc cttatgatgg aagggctcat ggcccgcgtc
      241 ttccacgacg ttctcggcga agagatccgg agccccatac cgcggatgac ctaccgggag
      301 gcgatggacc ggttcggggt cgacaagccc gacacccggt tcgggctgga gatcacggac
      361 cacacggcgc tcctgtcgaa gaccgaattc cgggtcttcg ccgaggtggc gtcgcgcggt
      421 ggcgtgatcc gggggatcac tgcgcccggc atgggcgaat cgagccgctc cgagatcgcc
      481 gcgctcgaag aggtggcgaa gatcgggggg gcctccggcc ttgcctcgtt caaggttacc
      541 gaggcggggc tgacctcctc cctggcgaag tttttccgcg cggagcagat ggcctccctg
      601 cgggaagcgg cgggcgcgca gccgggggac ctgattctcc tggtggccga ctcgttcgac
      661 gtggcgtgct cctccctcgg acgcctccgc ctccacctcg gggaaaagct cgggatgatc
      721 gacgcctccc tgcatcacct gttgtgggtg accgacttcc cgctcctcga gtgggataag
      781 gaggagaaga ggtggggggc gatgcaccac ccgttcacgg cgccgctcga cgaggacctc
      841 ccgctgcttg cgacggatcc cgggaaggtc cgcgccaagg cgtacgacat ggtgctgaac
      901 ggctcggaga tcggcggggg aagcatccgt atccaccgga aggacgtgca gtcacggatg
      961 ttcgatctgc tgaacatcgg accggaggat gcaagagtga agttcgggtt cctcctcgaa
     1021 gcgttggagt tcggcgcgcc gccgcacggc gggatcgcct tcggtctgga ccggctggcg
     1081 atgctcttct cgggcgcgaa atcgcttcgc gacgtgatcg ccttcccgaa gacccagaag
     1141 gcgacatgcc tcatgacgga ggccccgtcc ggggtcgact tgaagcagct gcgcgagctt
     1201 tcgatccggg tgacggtgcc ggagaagaag tagcccgcgc cggagagggg ccatgcttcc
     1261 tggcgaattg ttccatcccg gatcggtcgc cgtcgtcggg gcgtcccgaa acgcggagaa
     1321 agtcgggtac gcggtgttcg ccaacctcgt gcaggccggg ttccggggga agatctacgg
     1381 ggtgaatccc ggcggcggcg agcttttcgg ccacccgctg tacccgtcca tcgacgcgat
     1441 ccccgggccc gtcgacctgg gggtcttcgt cgtcccgccg aacgccatcc tcgaagggat
     1501 cccccggctc gcggcgaagg ggatgcgcgc ggggatcgtc atctccgccg gattcaagga
     1561 gatcggaggg gcgggggtcg ccctcgaacg ttccctgcgc gaggtggcga catccgcggg
     1621 ggtccgtgtg ctcggcccca actgcctcgg cctgatcgac acgcactcct cgctgaacgt
     1681 gtcgttctcc cgcgggacgc ccccgaaggg cttcatctcc ttcttctccc aatccggggc
     1741 gctcgccacc gcggtgctcg actgggccat cggccggaac atcggcttct ccaagttcgt
     1801 gagcctcggg aacaaggtcg acatctcgga gtcggacatc ct
//
LOCUS       DASVJL010000023         3471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_378308, whole genome shotgun sequence.
ACCESSION   DASVJL010000023 DASVJL010000000
VERSION     DASVJL010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378308"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2123)
                     /locus_tag="VF357_00430"
     CDS             complement(<1..2123)
                     /locus_tag="VF357_00430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7522638.1"
                     /translation="MIVQPRPAKEAVGRDVPPGTTGEGSPTGLLPRIAIVRGPNLNQW
                     EMQNYEMLAGSFDLTAYTTTTPNFDISGIRFPVVKVPPHPEHPGYMVGLESVLADADL
                     IYSADTTWMFSYQASLIREKHGKRLVCLQWENIPFAYEESGEMKELKAAVRKRADHFI
                     AVTERAKEALVLEGVDPGRITVIPMGIDTDRFMPDDMLREACRKELGISRDERMVLFT
                     GRMVWEKGVYDFVHAAKLAHSSTGKASVRFVMVGKGPERDAVMARAEEIGIAKWFLFI
                     EGHPYDRMRDLYNAADVFVLPSISTRMWKEQFGMVLAEAMACGTPVVSTTSGSIPEVV
                     GDAGILVPANDPGELAGAIALLCSAYELREELGRKSRTRAVERFDSRTVAKRVGEVFG
                     KVLEGSARPADSPRRRDIAPPPEPSNPRAAADILPVPRADAAPTPVLPDPGEEPRQDA
                     PKDHKYFQQERREIEAMIPAGASRILDIGCGEGMLGRILLQKGAAEVVGIEADLAVAQ
                     KAQENLSRVLQGDIESLVLPFEESYFDCIVLADVLEHLRDPLSTMIKLQRYLSDSGTI
                     VASIPNVRFFDVLRGLAEGRWEYQEFGILDKTHLRFFTKKEIEVLFLQAGLELTGISE
                     NLSPLYDTLPSAHSGDVSFGRVTLHGLTREETKDLFVCQYLIVARKADSEARGRDRRV
                     SAALASGDFESAGSLLEEFLEEHPL"
     gene            complement(2120..>3471)
                     /locus_tag="VF357_00435"
     CDS             complement(2120..>3471)
                     /locus_tag="VF357_00435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7522639.1"
                     /translation="EEAIGEITRRRTAAGYPPLAVFPHAPSVAAFPGYYVSVLEALSG
                     SAAVNLGDRLRYPKFQGDALTVALFDFGYFLTEEIARAVKALGHSVVKVHGRKDESCG
                     DILGRAVETIATRKPDFFLTVNHLGFDEEGVLADLFQSIEMPSAIWYVDSPNLIVRAF
                     PRNVSPFSSIFVWDEGYLDTMKSLGFENVSYLPLGVDETVFRPKSLSSPERKRMGTDL
                     GFVGNSMVGPARDQLAKVPRELHAAVERTANRMNLARNVPYGEAARESMSSDERAVFE
                     SLRESGKTGFEAAVLWRATLLYRLSCLRTLEEFRPCIRGDAGWKGLVNGKFRLGPQLN
                     YYKELPSFYNACTVNFNATSIQMGKAVNQRVFDVPACGAFLLTDHQESIEGLFEVGKE
                     VVTYKDRGEIADLARFYLRNGAARRTIAGKGRERVLAEHTYRHRIGTITQRLREVYG"
BASE COUNT          639 a         1138 c         1033 g          661 t
ORIGIN      
        1 tcgaggggat gctcctcgag gaactcctcc agcaatgacc ccgccgattc gaaatctccc
       61 gatgcgagag cagccgacac gcgacggtcc cgccctctcg cctcggaatc cgccttgcgg
      121 gccacgatca ggtattggca gacgaacagg tctttcgtct cttcccgggt cagtccgtga
      181 agcgtgacgc ggccgaacga gacgtccccg gagtgcgcgg agggaagcgt gtcatagagg
      241 ggagacaggt tttccgatat cccggtcaac tcgagaccgg cctggagaaa caggacctcg
      301 atctcctttt tcgtgaaaaa ccgcaggtgg gtcttgtcga ggataccgaa ctcctggtat
      361 tcccatcgtc cttcggcaag cccgcggagg acgtcgaaga accggacgtt cgggatgctc
      421 gcaacgatcg tccccgaatc ggacaggtat cgctgaagct tgatcatggt ggacagcggg
      481 tccctgagat gctccaagac gtccgcgagc acgatacagt cgaaatagct ttcttcgaaa
      541 gggaggacca gggactcaat atccccctgc aggacgcggg agaggttttc ctgcgctttt
      601 tgagcaaccg cgaggtccgc ttcgatcccc acgacttccg ccgctccctt ttggagaagg
      661 atccgcccga gcatcccttc tccgcagccg atgtccagga tccgcgacgc ccctgcggga
      721 atcatcgcct cgatctctct ccgctcctgt tggaaatatt tgtggtcttt cggggcgtct
      781 tgccggggct cctcaccggg atccggaagg accggcgtcg gtgccgcatc tgctcgtggg
      841 accggcagaa tgtccgccgc ggcgcgggga ttcgagggtt ccggtggggg agcgatgtcc
      901 cggcgccggg gagagtccgc aggacgagcg gaaccctcca ggaccttccc gaacacttct
      961 cccacccgtt tcgccaccgt cctggaatcg aatcgctcga cggcccgcgt ccggctcttt
     1021 cgccccagtt cctcgcgcaa ttcgtaggcg gagcacaaca aggcgatcgc gccggcgagt
     1081 tcccccggat cgttcgcggg aacgaggatc ccggcgtccc ccaccacctc gggaatcgag
     1141 ccggaggtcg tggagacgac gggggtaccg cacgccatcg cttcggcgag gaccatcccg
     1201 aactgttcct tccacatcct cgtggagatg cttggcagaa cgaagacgtc ggcagcgttg
     1261 tacagatcac gcatccggtc gtacgggtgc ccttcgatga agagaaacca cttcgcgatt
     1321 ccgatttcct cggctctggc catcaccgcg tcccgctcgg ggcccttccc caccattacg
     1381 aaccgcaccg aagccttccc cgtcgaggag tgcgcaagct tcgcggcgtg cacgaaatcg
     1441 tagaccccct tttcccacac catccgtccc gtgaagagta ccatgcgctc gtcgcgcgat
     1501 atcccgagct ccttccggca cgcttcccgg agcatgtcgt ccggcatgaa ccgatccgtg
     1561 tcgatcccca tcggaatgac ggtgatccgc cccgggtcca ctccttcgag gacgagggcc
     1621 tccttcgccc gctccgtcac agcgatgaag tggtccgccc gcttgcgtac ggccgccttg
     1681 agctccttca tctccccgga ttcctcgtag gcgaacggaa tgttctccca ctggaggcac
     1741 acgagccgct tcccgtgctt ctcccggatg agcgaagcct ggtatgagaa catccacgtc
     1801 gtatccgcgg agtagatgag gtccgcatcg gccagcacgg actcgagacc gaccatgtag
     1861 ccgggatgtt ccgggtgcgg cggcaccttg actacgggaa accggatacc gctgatgtcg
     1921 aagttcggtg tggtggtagt gtaggccgtc aggtcgaacg accccgcaag catctcgtag
     1981 ttctgcatct cccactggtt gaggttcggg ccccggacga tggcgatccg gggaagcagg
     2041 cccgtcggac tgccttcccc ggtcgtgccg ggtggcacat cccttccaac ggcttccttc
     2101 gccggtctcg gctggactat catccgtata cctccctgag gcgctgggta atcgttccga
     2161 tccggtgcct gtacgtatgc tccgccagga ccctctccct tccctttcct gcgatggtcc
     2221 tccgtgcagc gccattgcga agatagaacc tcgcgagatc cgcgatttcc ccccggtcct
     2281 tataggtgac gacttccttc cctacctcga agagcccctc gatcgactcc tggtggtcgg
     2341 tgaggaggaa ggcgccgcag gcggggacgt cgaacaccct ctggttgacg gccttcccca
     2401 tctggatgct ggtcgcgttg aaattgaccg tgcaggcgtt gtagaaggac ggcagctcct
     2461 tgtaataatt gagctggggg ccgaggcgga acttcccgtt cacgagccct ttccagccgg
     2521 catcgccgcg gatgcagggc cggaactcct cgagcgtccg caggcaggaa agccggtaga
     2581 ggagcgtcgc tcgccacagg acagcggcct cgaacccggt cttgccgctt tcccgcaatg
     2641 attcgaacac cgcccgttca tcggagctca tcgactcccg ggccgcctcc ccatacggga
     2701 cgttccgcgc gaggttcatg cgatttgcgg tcctctcgac cgcggcgtga agttcccgcg
     2761 gaaccttggc aagttgatcg cgtgccggac cgaccatcga attccccacg aacccgagat
     2821 cggtccccat ccgctttctc tccggggagg acaggctttt cgggcgaaag accgtttcgt
     2881 ccacaccaag aggaaggtac gaaacgttct cgaagccgag cgacttcatg gtatccaagt
     2941 acccctcgtc ccagacaaaa atggagctga agggtgaaac attccgggga aaagccctga
     3001 cgatgaggtt ggggctgtcc acataccata ttgcggacgg catctcgatc gactggaaca
     3061 agtccgccag gactccctct tcgtcgaaac ccagatgatt gacggtcagg aagaagtcgg
     3121 gcttgcgcgt ggcgatggtc tcgacggcac gtccgaggat atcgccgcac gactcgtcct
     3181 tcctcccgtg gaccttgacg accgagtgcc cgagggcctt cactgcccgc gcgatctcct
     3241 cggtcaggaa gtagccgaag tcgaaaaggg caaccgtcaa agcgtcccct tggaacttcg
     3301 ggtaccggag cctgtctcca agattgaccg cggcggaacc ggaaagcgcc tccaggaccg
     3361 acacgtaata gccgggaaaa gcggcaaccg atggggcgtg cgggaaaacg gcaagcggcg
     3421 gataccctgc cgcggtccgt cttctcgtga tctcgccgat cgcctcttcg g
//
LOCUS       DASVJL010000024         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_660301, whole genome shotgun sequence.
ACCESSION   DASVJL010000024 DASVJL010000000
VERSION     DASVJL010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660301"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF357_00440"
     CDS             complement(<1..180)
                     /locus_tag="VF357_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522640.1"
                     /translation="MNDSATTDSLLDTFLRLSLIEGFTVDHLRRLRASGAGGFLPGMT
                     PRGVSLLGKAKAALGS"
     gene            complement(259..1350)
                     /locus_tag="VF357_00445"
     CDS             complement(259..1350)
                     /locus_tag="VF357_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX7522641.1"
                     /translation="MKHAAVNMGTFAVVCMLILLPVGAFAQQAPSGTANSSEGIVHTV
                     VEGDTLWDLSAKYLGSPWKWTTIWERNRFITNPHYIYPGILVVIVPPGPREIALGQEP
                     TPASGPAETGAAVVPPMDAVKPAVSSVAPPRVGFLDIKPEDFVRAGEFLKEAPKGIGY
                     IQGGKDPKVGFVEGDTVYLSLRKEIPPGQLLGVYRVRGPIDSPGKRSVSGYVKYLIGV
                     IQAVPNVDGQATARVRHSFEDLSRDDLISEEIPAYTPVRIDPGADGIPSSVITGRLEN
                     KELAQGDFVYLDQGASAGVAEGNVFRLFVPTGEAAGASSSYASGTVLFEVARAVVVRV
                     SPDFSTAYITRGSESFAAGVSARRGIPSK"
BASE COUNT          301 a          554 c          482 g          247 t
ORIGIN      
        1 ggagccgagc gccgccttcg ccttcccgag aagggaaacg ccccgcggcg tcatgccggg
       61 gaggaaaccg ccggccccgg aggcgcggag gcgccggaga tgatcgacgg tgaacccctc
      121 gatcagcgac agtcggagga acgtatcgag gagggaatcg gtggttgcgg aatcattcac
      181 gggggaaccc ctggccggtt tcccccgtta tgattcaaat gtcgtgccgt cgttgaaccg
      241 ccagggacgg cggaaaatct acttcgaggg tatcccccgc cgggccgata cgcccgcggc
      301 gaacgactcc gagccgcggg tgatgtacgc cgtggagaag tccggcgaca cacgaaccac
      361 gaccgccctt gccacctcga acaggaccgt gccggaagcg taagacgacg acgcccctgc
      421 cgcctccccg gtcgggacga acaggcggaa gacgttcccc tccgccacgc ccgccgacgc
      481 gccctggtcg aggtagacaa aatcgccctg cgcaagctcc ttgttctcga gccgcccggt
      541 gatcaccgag gacgggatgc cgtccgctcc cgggtcgatc cgcaccggcg tgtacgccgg
      601 gatctcctcc gagatcaggt cgtcacgcga gaggtcctcg aacgaatgcc gcacccgcgc
      661 ggtcgcctgt ccgtcaacgt tcggcaccgc ctggatcacc ccgatcaggt atttcacata
      721 tccggaaacc gaacgcttcc ccggactatc gatcggtccg cggacccggt agacgcccag
      781 aagctggccc ggcgggatct ccttccggag cgagaggtag accgtgtccc cttcgacgaa
      841 tccgaccttc ggatccttcc ctccctggat gtacccgatc cccttcggcg cctccttcaa
      901 aaactccccg gcacggacga aatcctccgg cttgatgtcg aggaacccca cgcggggagg
      961 ggcaacggac gaaaccgcgg gcttgaccgc atccatgggc ggaacgactg cggcacccgt
     1021 ttcggcgggc ccggaggcgg gcgtcggctc ctgcccgagc gcgatctccc tcggacccgg
     1081 cgggacgatc acgaccagga tgccgggata gatgtagtgc ggattggtga tgaagcggtt
     1141 tcgctcccaa attgtcgtcc atttccacgg ggacccgagg tatttcgcgg aaagatccca
     1201 gagggtgtcc ccctcgacca ccgtgtggac gatcccctcg gacgagttcg ccgttcccga
     1261 cggcgcctgc tgcgcaaacg caccgaccgg gaggaggatc aacatgcaca ccaccgcgaa
     1321 cgtccccatg ttcaccgccg catgcttcat cgtccgcacc ccgtttcccc gcccgaaagg
     1381 cgttatgtac cactcttatc ggtgaaacgt ccgcgcatat tcaagaaaat tacctgccgc
     1441 cccccccgaa gtcaagggga cctttcagca aacgggcgcc cgcaccgcag agcgcacccc
     1501 actcccgtac taaatgcgct cccctgaggt acccccgggc tcgatatgag gggcacgacg
     1561 cagatgacgt ttgttgggcg gaga
//
LOCUS       DASVJL010000025         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1035632, whole genome shotgun sequence.
ACCESSION   DASVJL010000025 DASVJL010000000
VERSION     DASVJL010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035632"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1105
                     /locus_tag="VF357_00450"
     CDS             143..1105
                     /locus_tag="VF357_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7522642.1"
                     /translation="MIRKGPILSIEGLSKSYPTGFWRKPVRVLSDLSCEVRENEIVGF
                     LGPNGAGKTTTIKILNRLAFPDAGKVTLFGGQVRDGADVRRRIGFMPEQPYFYEYLTG
                     GEFLRLCGQLCGMSRADTELHSSELLSRVGLSGARGTAIRKYSKGMMQRLGLAQALLH
                     DPELVILDEPMSGLDPMGRMEVRGIIRDLKAAGKTVFFSSHILSDVEALCDRVIMLHK
                     GRKVAEGRVEELIGAETLYIELVVSPVLPADRLAAAGIPPEAGYAQGALLVLRAPDNN
                     QANQWIATLLREGCSIISCVPMKKGLEEIFMERVGSPAATGAAS"
     gene            1107..1883
                     /locus_tag="VF357_00455"
     CDS             1107..1883
                     /locus_tag="VF357_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7522643.1"
                     /translation="MFRRIFSIAANTFRETIRNKILYAIIAFALFVIGMTFFLADLSV
                     GDFARIIADVGLASIHVFGVIMAVFLGINLVSNEVDRKTIYIILSKPVRRYEFIFGKT
                     LGLSVTLLLTTLAMAAVLFLVHLSYQYGGRAEVGIFIASAGIYMELVLLTCLASLFST
                     FTTPVLSAIFTLSLFLVGHLTKYLYVLGEQSKVGAVRWGSRFLFYLLPNLENFNWKNE
                     VAYGNLKSFSILGWAAGYLVCYAACVLCLSCLLFARKDFK"
     gene            1880..2641
                     /locus_tag="VF357_00460"
     CDS             1880..2641
                     /locus_tag="VF357_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX7522644.1"
                     /translation="MTFIFSLSAAILVGVCIGSFLNVVIHRLPRGESIISPRSRCPDC
                     GRSIRAWENIPIVSFVALGGKCAGCGGAISLRYPAVELLTAAGFGAIFHLDGPGIPLL
                     RDLLFFSLLIPIAFIDIDHRIIPDELSLGGLAAGLLLSFLPGGDWKGSFTGALLGGGI
                     LYATAFLYEKIRGAEGMGGGDIKLLAMIGAFVGWRGTLATIFFGAFLGAVGGMLAMRK
                     GEEGLKTAIPFGPYLCAAALGARYLGGWFWRWILI"
     gene            complement(2634..3065)
                     /locus_tag="VF357_00465"
     CDS             complement(2634..3065)
                     /locus_tag="VF357_00465"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEX7522645.1"
                     /translation="MNLFRFRRISLKPTLAEHRTGRRGFTLIEITIVLLLIGILAIIA
                     VPTYRMVVSKARMTQAKTILGHLTKTEATYFSGNDRYTDNVVLLDFDPVTYDYYRVSV
                     VLDNDAKNYTGTATGIGVMMGDSWTITKDGQPVQADNSAFR"
     gene            complement(3062..>3472)
                     /locus_tag="VF357_00470"
     CDS             complement(3062..>3472)
                     /locus_tag="VF357_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522646.1"
                     /translation="RLLLVLTILFAAGLLLAFAPSYYILATTNDLKSVEPAAAEFLVL
                     HKRFLPAALLSLAGIFIYAIFFSRRITGTLHGINAILQALIEDRDPPVVKLPEDDYFH
                     PTAALLKELSEKMRASRGTTASRPGGNPPPGEAK"
BASE COUNT          577 a         1015 c         1151 g          729 t
ORIGIN      
        1 cgggagatcc gtccgccctc gtccgggagg gaatcctgtc gagcataccg gaggagccca
       61 acggggggag atacctcatg gagccggggg gaaaggtgcg cagcgaccgg gtctcggagc
      121 ggctccgggt gtttcagtcc aaatgatacg caaagggccg atcctctcca tcgaaggtct
      181 gtcgaaatcg tacccgaccg gtttctggcg gaaaccggtt cgcgtgctgt ccgatctttc
      241 ctgcgaggtg cgcgagaacg agatcgtcgg gtttctggga cccaacggcg ccgggaagac
      301 cacgacgata aaaatcctga accgcctcgc gttcccggac gcgggaaagg tgaccctgtt
      361 cggcgggcag gtccgggacg gggccgatgt tcgccgccgc atcggcttca tgccggagca
      421 gccgtacttc tacgaatacc tcaccggcgg ggagttcctt cgcctttgcg ggcaactgtg
      481 tggaatgtcg cgggcggaca ccgaattgca ctcttcggag ctcctctccc gggtcgggct
      541 ttcgggggcc cggggcacgg ccatccggaa atattcgaag gggatgatgc agcggctggg
      601 gttggcccaa gcactgctac acgacccgga gctcgtcatc ctcgacgagc cgatgtccgg
      661 gctggacccg atgggccgga tggaggtccg cgggatcatc cgggatctca aggcggcggg
      721 gaagaccgtg ttcttcagct cccacatcct ctccgacgtg gaggccctct gtgaccgggt
      781 catcatgctg cacaagggac ggaaagtggc ggagggtcgc gtggaggagt tgatcggggc
      841 ggagacgctc tacatcgaac tggtcgtctc gccggttctc ccggcggacc ggctcgcggc
      901 ggcgggaatt cctccggagg ccgggtacgc gcagggggct ctcctcgtgc tgcgggcgcc
      961 ggataataac caagcgaacc aatggatcgc gactcttctg cgtgagggtt gctcaatcat
     1021 ttcctgcgta ccgatgaaga agggccttga ggagatcttc atggagcgtg tcgggagccc
     1081 cgcggcgacg ggggcggcgt cctgacatgt tccgcagaat cttctccatc gccgctaaca
     1141 ctttccggga aacgatccgg aacaagatcc tctacgcgat catcgccttt gccctcttcg
     1201 tcatcggcat gactttcttc ctcgccgacc tgtcggtggg cgacttcgcc cggatcatcg
     1261 ccgacgtggg gctggccagc atccacgtat tcggggtcat catggcagtc ttcctcggga
     1321 tcaacctcgt cagcaacgaa gtcgaccgga agacgatcta catcatcctc tcgaagcccg
     1381 tacggcgcta cgagtttatc ttcgggaaga cgctgggatt aagcgtcacc cttctgctca
     1441 cgacgcttgc gatggcggcg gtcctcttcc tcgtgcacct ttcctaccaa tacggcggga
     1501 gggcggaggt cggcatcttc atcgcctccg cgggtatcta catggagctg gtgctgctca
     1561 cgtgcctggc ctccctcttc tcgacgttca ccacaccggt attgagcgcc atcttcacgc
     1621 tctccctgtt cctcgttggc catctgacga agtatctcta tgtccttggg gaacagtcga
     1681 aagtgggagc ggtgcggtgg gggagccgtt tcctgtttta tctcctgccg aacctcgaga
     1741 acttcaactg gaagaacgag gtggcgtacg ggaatctgaa atcgttctcg atcctcgggt
     1801 gggcggcggg gtatctcgtc tgctacgcgg cgtgtgtcct ctgcctctcg tgtctcctct
     1861 tcgcccgcaa ggatttcaag tgacgtttat cttctccctt tccgctgcga ttctcgtcgg
     1921 ggtgtgcatc ggatcttttc tgaacgtggt gatccaccga ctgccgcgcg gggagtcgat
     1981 tatctccccc cggtctcggt gccccgactg cggccggtcg atccgggcgt gggagaatat
     2041 ccccatcgtc agtttcgttg ccctcggcgg gaaatgcgca ggatgtggag gggcgatctc
     2101 cttgcggtat ccggcggtcg aactgctcac agcggcgggg ttcggggcga tctttcatct
     2161 ggacgggccc gggatcccgc tgctgcgcga cctcctcttc ttctcccttc ttataccgat
     2221 cgccttcatc gacatcgacc accggatcat ccccgatgaa ctctccctcg gcgggctcgc
     2281 cgcgggcctc cttctttctt tcctccccgg aggggattgg aaggggagtt tcacgggtgc
     2341 gctgctgggg ggcgggatcc tttacgccac tgcgttcctg tacgagaaga tccgcggggc
     2401 cgaggggatg ggaggcggcg acatcaagct gctggcgatg atcggcgcgt tcgtggggtg
     2461 gcgcggtacg cttgcgacga tcttcttcgg cgcgttcctc ggtgcggtcg gggggatgct
     2521 ggcgatgcgg aaaggcgagg agggactgaa gacggcaatc ccgtttgggc cctacctgtg
     2581 cgcggccgcc ctcggggcgc ggtatctggg ggggtggttc tggaggtgga tcctcatctg
     2641 aacgccgagt tgtcggcctg gaccggctgc ccgtccttgg tgatcgtcca cgagtccccc
     2701 atcattacgc cgattcccgt tgcggtcccc gtgtaattct tcgcgtcgtt gtcaaggacc
     2761 accgagaccc ggtagtagtc gtacgtcacc gggtcgaaat cgagcagcac gacgttgtcc
     2821 gtgtaccggt cgttgccgct gaagtaggtg gcctctgtct tggtgaggtg gccaagaatg
     2881 gtttttgcct gcgtcatccg cgctttggag acaaccatac gatacgtggg aacggcgatg
     2941 atcgccagga tgccgatgag caggaggacg atcgtgatct cgatcagtgt gaacccgcgc
     3001 ctgccggtgc gatgttcggc aagggtgggc ttcagggaga tccttcggaa ccggaacagg
     3061 ttcatttcgc ctcgccgggc gggggattgc cgccggggcg ggatgcggtg gttccccgcg
     3121 aggcacgcat cttctccgac aattccttta atagtgcagc ggtgggatgg aagtagtcgt
     3181 cttcggggag tttcactacg gggggatccc ggtcttcgat gagcgcctgg aggatcgcgt
     3241 tgattccgtg caacgtcccc gtgatccgtc ggctgaagaa gatcgcataa ataaagatcc
     3301 ctgcaaggga gaggagcgcc gcgggcaaga agcgcttgtg aagcacgagg aactcggcgg
     3361 cggcgggctc gacggatttc agatcgttcg tcgtcgcgag gatgtaatac gacggagcaa
     3421 acgcgaggag gagccccgcc gcgaaaagga tcgttagcac gaggaggagc cg
//
LOCUS       DASVJL010000026         3106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_97922, whole genome shotgun sequence.
ACCESSION   DASVJL010000026 DASVJL010000000
VERSION     DASVJL010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97922"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VF357_00475"
     CDS             complement(<1..583)
                     /locus_tag="VF357_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX7522647.1"
                     /translation="MPDRARGGIAQGVLEELRPIFTTKMVLSAWIPCLVITVLHYRTG
                     AQHPWGHDILRRLYYLPILFATFSGGLRGGVSVSVFASLVYLPHAFTSLLVQDPADAL
                     EKGLEVILYNIVAVVAGLLVDRERREREQQERLATRLGDALAEQRRIEVQLIRAGKLG
                     ALGEMTAGIAHEIKNPLHALKGTAEILRDAVPEG"
     gene            complement(576..1940)
                     /locus_tag="VF357_00480"
     CDS             complement(576..1940)
                     /locus_tag="VF357_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7522648.1"
                     /translation="MSGRILFIDDDPAGREVALFNLRKAGYEVSAAEDGARGLSAFAA
                     APFDLVITDLKMPGIPGMEVLRKVHAGSPDVPVLVITAFGNVETAVAAMKEGAYDFIG
                     KPFHRDQLLHAVEKALERRRLATEVHDLRIRASGVGRDVVSVSAAMRRLLEVADRVAP
                     TDATVLITGESGTGKEVLARRIHVRSLRAEGPFVAVDCAAIPAELLESELFGHARGAF
                     TGAVRDRPGRFRQASGGTLFLDEVAEIPTALQGKLLRALQERVVDSVGGDAPVPVDVR
                     ILAATNRDLGARIRDGSFREDLYYRLNVVELVVPPLRERPDDIPPMVECFVLEFAAGR
                     EITVPAAVVAELSRRPWPGNVRELKNACERMVILCRGDEVSLDDLPPAPGGSGAGTAE
                     DASAAFPPLPAEGLSLVDLEKRVIEGALRMKGGNITQAALFLRIPRHILVYRIGKYGL
                     RRDA"
     gene            2027..>3106
                     /locus_tag="VF357_00485"
     CDS             2027..>3106
                     /locus_tag="VF357_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX7522649.1"
                     /translation="MVFFIGEPTSRKEGNVRIRRFDKKKFLLFLSVLGPGIITASVDN
                     DAGGIATYSIAGAHFGYALLWTLIPITVALIVVQEMVARMGVVTGKTLADLIREKFGV
                     RPTFFLLVALVLANLGNTVAEFAGWASAWEIFGVSKYFSVPVGAAAVWFLVVKGTYRV
                     VEKIFLVACLIFFAYPVAAYLAGPDGAVVARNLVVPDIHVNAAYITLLIGMVGTTIAP
                     WMQFYLQSAVVEKNLQVEEYPLTRIDVIFGCLVTDIVALSIIVACGATLYVKGIHIED
                     AKDAAVALAPLAGKYASTLFAIGLANASLFAASILPLATAYCVCEGMGWESGIDKDFR
                     TAPQFFWLYTGLIVIGGALVLYPRAP"
BASE COUNT          492 a         1070 c         1038 g          506 t
ORIGIN      
        1 ccccttccgg gacggcgtcg cgcaggatct ccgcggtccc cttcagggcg tggagcgggt
       61 tcttgatctc gtgggcgatc cccgccgtca tctcgcccag cgccccgagc ttccccgcgc
      121 ggatcagctg cacttcgatc cgccgctgtt ccgcgagagc gtctccaagc cgggtcgcca
      181 gccgttcctg ctgctcccgc tcgcggcgct cccggtcgac caggaggccc gccacgaccg
      241 ccacgatatt atagaggata acctccaaac ccttctccag cgcgtccgcc gggtcctgca
      301 ccaggaggct cgtgaaggcg tgggggagat agaccaggga ggcgaagacc gaaacggaga
      361 ccccgccccg caagccgccg gagaacgtcg cgaacaggat cggtaggtag tagagccggc
      421 ggaggatgtc gtgaccccat ggatgctgcg ccccggtacg gtaatgcagc acggtgatca
      481 cgaggcacgg gatccacgcg gagagcacca tcttcgtcgt gaagatcggc cgcagctcct
      541 ccagcacccc ttgggcgatt cccccacgcg cgcggtcagg catcgcggcg aagtccgtat
      601 ttcccgatcc ggtagacgag gatgtgccgg gggatccgca ggaacaaagc cgcctgcgtg
      661 atgttccccc ccttcatccg caacgcgccc tcgatcaccc gcttctcgag atccaccagg
      721 gaaagtcctt cggccggaag cggggggaac gcggcggaag cgtcttccgc ggtccccgcg
      781 cccgatccgc ccggcgcggg cgggagatcg tcgagggaaa cctcgtctcc gcgacagagg
      841 atgaccatcc gctcgcacgc gttcttcagc tctcgcacgt tgcccggcca cgggcgcctc
      901 gaaagctcgg cgaccacggc ggcgggaacc gtgatctccc gtccggcggc gaattcgagg
      961 acgaagcatt cgaccatcgg ggggatgtcg tccgggcgct cccgcagcgg cgggacgacg
     1021 agttccacga cgttcaagcg gtaatacagg tcctcccgga aggaaccgtc ccggatccgc
     1081 gcgcccaggt cccggttcgt ggcggccagt atccggacgt ccaccggcac cggcgcgtcg
     1141 ccccccaccg aatcgaccac cctctcctgg agcgcccgaa gaagcttccc ctgcagcgcc
     1201 gtcgggatct cggccacctc gtcgaggaag agcgtcccgc ccgacgcctg gcggaaccgc
     1261 ccggggcggt cgcgcaccgc ccccgtgaag gcgccgcggg cgtggccgaa cagctccgac
     1321 tcgagcagct ccgcggggat cgcggcgcag tcgaccgcca cgaagggacc ctcggcccgg
     1381 agggaccgca cgtggatccg ccgggcgaga acctcctttc ccgtgccgct ctcgccggtg
     1441 atgaggaccg tggcatcggt aggagccacg cggtctgcca cctcgagaag ccgccgcatc
     1501 gccgcggaaa cggagacgac gtcccgcccg acgccggagg cgcggatgcg caggtcgtgg
     1561 acctcggtgg ccagccggcg ccgctcgagc gccttctcca ccgcatgcag gagctggtcc
     1621 cggtgaaacg gcttcccgat gaagtcgtac gccccctcct tcatcgccgc cacggcggtc
     1681 tcgacgtttc cgaaggcggt gatgacgagg acggggacgt cgggcgaccc ggcgtggacc
     1741 ttccggagca cctccatccc ggggatgccg ggcatcttga ggtcggtgat caccaggtcg
     1801 aagggggcgg ccgcgaacgc ggacagcccc cgggccccgt cttccgccgc ggacacttcg
     1861 tacccggcct tccggaggtt gaacagcgcc acctcgcggc ccgcggggtc gtcgtcgatg
     1921 aagaggatgc gcccggacat ggaagcctct cattggcctt tcttctccga ttttacagga
     1981 aagctcccgg gaaatcgcct tttgcggggc ggcggacgcg ggaggtgtgg tatttttcat
     2041 cggagaacca acgtcacgaa aagaggggaa cgtgcgcatc cgccggttcg acaagaagaa
     2101 gttcctgctg ttcctgtcgg tcctcggccc ggggatcatc acggcgtccg tcgacaacga
     2161 cgcaggcggg atcgccacgt actcgatcgc cggggcgcac ttcgggtacg cgctcctttg
     2221 gacgctcatc cccatcacgg tcgcgctgat cgtcgtccag gagatggtcg cgcggatggg
     2281 cgtggtcacg gggaagacgc tggcggacct catccgggag aagttcggcg tccgcccgac
     2341 cttctttctc ctggtggcgc tggtgctcgc caatctcggg aacacggtcg cggagttcgc
     2401 cgggtgggcc tccgcgtggg agatcttcgg cgtcagcaaa tatttttccg tgcccgtcgg
     2461 cgcggcggcc gtctggttcc tcgtggtgaa ggggacgtac cgggtcgtgg agaagatctt
     2521 cctcgtggcg tgcctcatct tcttcgccta tccggtcgcc gcgtacctcg cgggccccga
     2581 cggcgccgtc gtggcgcgca acctggtggt gccggatatt cacgtcaacg ccgcatacat
     2641 caccctcctg atcggaatgg tgggaacgac gatcgccccc tggatgcagt tttacctgca
     2701 gtccgccgtg gtggagaaga acctgcaggt ggaggagtac ccgctgacgc ggatcgacgt
     2761 gatcttcggg tgcctcgtga ccgacatcgt ggccctgtcg atcatcgtgg cgtgcggcgc
     2821 caccttgtac gtaaagggga tccacatcga ggacgccaag gacgccgccg tggcgctggc
     2881 tccgctggcc ggaaaatacg cctccacgct gttcgcgatc gggcttgcga acgcgtcgct
     2941 gttcgccgcc tcgatcctgc cgctggccac cgcctactgc gtgtgcgaag gaatggggtg
     3001 ggagtcgggg atcgacaagg acttccggac ggccccccag ttcttctggc tctacacggg
     3061 gttgatcgtc atcggaggag cgctggtcct ctacccgcga gccccg
//
LOCUS       DASVJL010000027         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1129693, whole genome shotgun sequence.
ACCESSION   DASVJL010000027 DASVJL010000000
VERSION     DASVJL010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129693"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(479..>1798)
                     /locus_tag="VF357_00490"
     CDS             complement(479..>1798)
                     /locus_tag="VF357_00490"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX7522650.1"
                     /translation="ALLENPVPYEQMFESLLAAVRSIAGPRSAGVAGSPPYALISKEI
                     VEVGRGLGLGRIVVDGLRIAAHLLTPPMEGENGATAPAPRSGANLFGDLDASIRVARS
                     LAFPWDIAACLQLLGTVTPDGGSASRPEEERKVLSTAAGLLALVWYRHQALTSLRAGS
                     GGDPEALKSGLRAQAGRLAPSSVVEAYVRVLEQFDAFSGAGKDIVLVGEADPRSSNLV
                     LELKQHGFRVAQADDLAEARKICLRRRPAAIVLRVDESLSAADAFCRFLREEEGDADT
                     VLFAVTQRSEPSFLLNLMDTWFSDVFTLPMNGQVVVARISRALSGREKGAGGPARHGF
                     SATFKDLSFVDLVQALGTGGKNVRMRIEHASGMQADICFRQGRIVFAECGDEKGVDVV
                     YRVICWQDAGNFRIEPIRSFPPENVLVPTDYILLEGSRRLDEHQAGR"
BASE COUNT          311 a          606 c          605 g          276 t
ORIGIN      
        1 cgcagctcgt cgcgttccgt accctcggtc ggttcacggc gaccgcgttt tggggacgcc
       61 cgggacggtt tcggcgaatc gaaccaggtc gacctcgagc gctggctcgc ccttcccacg
      121 ctgctccgat ggtccaatac ccttgatatc tcggagaaga gcaacgggtg gacgtggggg
      181 acggagctct tcctcctcca caagatttcc ccgaaaagcg caatcacctt cgccgggggc
      241 acctcggcgc tcgacccggc ctgcgtggat cgcacagaat taccgcatcc ttgtccggta
      301 ccgccggaac gcctggcgga aatggctctt cctcgagggg gagcccgacg tccactggcc
      361 gaggaaggaa gacgggagcc ggaagccggt gtggggcgcg acgctgcggg cggaaatcct
      421 gttcaccggg gtggggccga atccgccgga cgatgaaggg agcaggcctt gacgggggtc
      481 aacgcccggc ctggtgctcg tcgaggcgac gggatccttc caggaggatg tagtcggtcg
      541 ggaccaacac gttctccggg ggaaacgatc ggatcggctc gatccggaag ttcccggcgt
      601 cttgccagca gatgacccgg tagacgacgt cgacgccttt ctcgtccccg cattccgcaa
      661 aaacgatccg cccctgccgg aaacagatgt cggcctgcat cccgcttgcg tgttcgatcc
      721 gcatgcgaac gttctttccg ccggtcccca gcgcctggac gagatccacg aacgacagat
      781 ccttgaaggt ggcgctgaat ccgtgccttg ccgggccgcc ggctcccttc tcccggccgg
      841 agagcgccct ggagatccgg gcgacgacca cctggccgtt catgggcaag gtgaacacgt
      901 cgctgaacca ggtatccatg aggttcagga ggaacgacgg ctcgctccgt tgcgtcacgg
      961 cgaacagcac cgtgtcggcg tctccctcct cttcgcggag gaaacggcaa aacgcgtcgg
     1021 cggcggacag cgactcgtcg acgcgaagga cgatcgccgc cggcctgcga cgaaggcaga
     1081 tctttcgcgc ctccgcgagg tcgtcggcct gcgcgacccg gaatccgtgc tgcttcagct
     1141 cgaggacgag gttcgacgac cggggatccg cttcgccgac gagaacgatg tccttgccgg
     1201 cgccggagaa ggcgtcgaac tgctcgagca cccggacgta cgcctcgacc acggacgatg
     1261 gcgccaggcg tcccgcttgg gcacggagcc cggacttgag ggcttcggga tcccccccgg
     1321 aaccggcgcg cagggacgtc aacgcctgat gccggtacca gacgagcgca aggaggccgg
     1381 ccgcagtgga caacaccttt ctctcttcct ccggccggga ggcgctgccg ccgtccggag
     1441 tcaccgttcc gaggagttgc aggcaggcag cgatgtccca gggaaacgcg agagaccggg
     1501 cgacccggat cgacgcgtcg agatccccga acagattcgc gcccgaccgg ggggcggggg
     1561 ccgtcgcccc gttctctccc tccatcggcg gggtcagcag gtgcgccgcg atccgaaggc
     1621 cgtcgacgac gatgcggccc agacccaggc cccggccgac ctccacgatc tccttcgaga
     1681 tcagcgcgta cggtggggat ccggcaacac ccgcagagcg tggcccggcg atgctccgga
     1741 ccgccgccag cagggattcg aacatctgtt cgtaggggac cggattctcg aggagagc
//
LOCUS       DASVJL010000028         1779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_98155, whole genome shotgun sequence.
ACCESSION   DASVJL010000028 DASVJL010000000
VERSION     DASVJL010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98155"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="VF357_00495"
     CDS             complement(<1..1528)
                     /locus_tag="VF357_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX7522651.1"
                     /translation="MSREARVGIFVLLGLIVLTFFTFRVSKWGLIAEKGYRLTVDFDS
                     AAGLEPKSDVKMAGVPIGKVEEIQLVGNRARLILRIQQKIRIPIDSVGTIQTQGLLGE
                     KYVEILPGKDVQRNLPAGGQVANTLSPTNLDEMVRKLSAIGDDVKKFTETLSATFGTE
                     EGKKALGDILRDIRATTATLRTVMTGNEQRFDRILANIDRLSADLSDISVSNKQDVRA
                     TIANLRAFSDTLKSETPALVSKLEEMSDRVSGVVGDNRENLKESIQNFKTASARLDNT
                     LDAAGRVMAKIDRGEGTLGMLVNDNTAHGSLTDTLEGINRYVRKTEQLKAFIDYRLEW
                     EAGPSDFKHYVNLRLQPTADKYYLIGVVDDPRGKFSSSTTTVTVNGTPSTISEEKFDN
                     KLKVSALIARRFSGLTVKGGVIESTGGLGLDYELLKNRMTVGADVWDFTRKELSGKSL
                     PPHLKLYGNYDIVKNLFVTGGVDDVLATEKNLRTLFFGFGIKFADEDIKTVLGAVPIK
                     P"
BASE COUNT          283 a          580 c          538 g          378 t
ORIGIN      
        1 acggcttgat gggaacggcc cccaggacgg tcttgatgtc ctcgtcggcg aacttgatcc
       61 cgaaaccgaa aaacagggtc cgcaggttct tctcggtcgc cagcacgtcg tccactccgc
      121 cggtcacgaa aaggtttttg acgatatcat agttgccgta cagcttcaaa tgggggggca
      181 gggacttgcc ggagagttcc ttgcgggtga agtcccagac gtccgccccg accgtcatcc
      241 ggttcttcag aagttcgtag tcgaggccta ggccgccggt ggattcgatc acgccaccct
      301 tcaccgtgag gccggagaat ctcctggcga tcagggcgga gaccttcagc ttgttgtcga
      361 acttctcttc cgaaatggtc gagggcgtgc cgttgacggt gaccgtcgtc gtgctggagc
      421 tgaacttccc gcgcggatcg tccaccactc cgatcaggta atacttgtcc gccgtcggct
      481 ggagccggag gttcacgtag tgcttgaagt cggacggccc cgcctcccac tccaggcggt
      541 agtcgatgaa cgccttcagc tgctccgtct tccggacgta ccggttgatc ccctcgaggg
      601 tgtcggtgag ggagccgtgg gccgtgttgt cgttgacgag cattccgagg gtcccctcgc
      661 cgcggtcgat cttggccatt accctcccgg cggcgtccag cgtgttgtcg aggcgcgcgg
      721 aggcggtctt gaagttctgg atgctctcct tcaggttctc gcggttgtcc ccaacgacgc
      781 cgctcacccg atcgctcatc tcctcgagct tgctcaccag cgccggcgtc tcgcttttca
      841 gcgtgtcgga aaaagcccgc aggttggcga tcgtcgcgcg gacgtcctgc ttgttgctca
      901 cggagatgtc ggaaaggtcc gcggagagcc ggtcgatgtt cgccaggatc cgatcgaagc
      961 gctgttcgtt ccccgtcatg acggtccgca gggtcgctgt ggtggcccgt atatcgcgaa
     1021 ggatgtcccc gagcgccttc ttcccctctt ccgtcccgaa ggtggcggag agcgtttcgg
     1081 tgaacttctt gacatcgtcc ccgatcgccg acagtttcct caccatctcg tcgagattcg
     1141 tggggctcag ggtgttggcg acctgccccc cggccggcaa gttgcgctgc acatccttgc
     1201 ccgggaggat ctcgacgtat ttctcgccga ggaggccctg tgtctggatg gtgcccaccg
     1261 agtcgatcgg gatacggatc ttctgttgga ttcgcaggat caggcgggcg cggtttccga
     1321 ccagttggat ctcctcgacc ttcccgatcg ggaccccggc catcttcacg tccgacttgg
     1381 gctccagccc cgccgccgaa tcgaagtcca ccgtgagccg gtatcccttc tcggcgatca
     1441 acccccactt gctcacacgg aacgtgaaga acgtgaggac gatcagcccc agaaggacga
     1501 agatcccgac gcgggcctcc ctggacatcg acgatgctcc cccctgtaat gtggcccgac
     1561 ggaaacccgc cggccggact tctcgccccg aagatcgggg atactatatc atcggctgac
     1621 gaagcggacg aactcctcgc tctcgtcggt gatggggccg tgcgcgcgcc cctgcacgaa
     1681 ctgcgcgacc accgggttcg acgtcgtccg gatctcctcc ggcgtcccca cctcgatgat
     1741 cttccccttg tacagcatcg cgatgtgatc cgcgatctt
//
LOCUS       DASVJL010000029         6451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1317604, whole genome shotgun sequence.
ACCESSION   DASVJL010000029 DASVJL010000000
VERSION     DASVJL010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317604"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VF357_00500"
     CDS             <1..1325
                     /locus_tag="VF357_00500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7522652.1"
                     /translation="DLPDVPRDVSDGGVDLCEGEAQRLAHTAILFESGRRGKPRISCG
                     KIASPMAPSSAPLYGRDYILLNAVNFLYSLYSVIFIFLPAYMYRLGIREGAIGVLMAT
                     GALVSVLLKPGLGMVVGRGARRSFLSLGAFLAAASTVPWLFVTAPGAHLFVIRVAQGA
                     SFSIFTAASYAYIAASAPPARRAEALGIFGLSFFLPVSVGGWLGEWVIGRAGFHGLFA
                     AGIGIAFLAALFPLAMREPSVRERPSMASLKIFLSRPFLLPNTAGYLFGVAYGSIFTF
                     LPVYLVVRGSESLGAFVFVYALSVIGTRMFGRKMLDRLPREWVSLCALVLMGAGNLLI
                     PAVPGGVGIVLVGAAAGAGHGLLFPALSALVLDRVGEGSCAMAMAMFTAAFDMGLVTG
                     AAAFGFVAERFGYEAMFVTAAAVVGAGTAAFFVLDPAFRKAARPEPAP"
     gene            complement(1291..1995)
                     /locus_tag="VF357_00505"
     CDS             complement(1291..1995)
                     /locus_tag="VF357_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7522653.1"
                     /translation="MTARTVFHGVFLGLVTLGIGLPAIALGMLVPGRSRKGRIFRWVT
                     KSYAMILLPVFGVTVETRGVSRVDRSAPYVFMSNHASHIDSLALAVSIPHPLHWVFKK
                     ELSKIPVFGWVLLSLGQIMVDRRNAMQSRTALSNAAAALAGNNSVLIYVEGTRSKDGK
                     LQPLKKGGFYIALKAGLPIVPVRVSGSHDIVPPGSLRIRPGRVVVEMFDPIPTEGKTE
                     ADVPELMARVRDALLS"
     gene            complement(1992..2750)
                     /gene="nth"
                     /locus_tag="VF357_00510"
     CDS             complement(1992..2750)
                     /gene="nth"
                     /locus_tag="VF357_00510"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX7522654.1"
                     /translation="MAGAARGLSARLRDVSGLLARRFGRPDICAHETDPVRNLVLTIL
                     SQNTTDANRDRAYDRLAKRFPSLPALAAARPSELEEAIRVGGLAKAKAKSILGALARI
                     REERGGYSLDFLRGMPLPEAREYLTSFPGVGVKTANILLLFSFGMPAFPVDTHVLRVT
                     KRLGLVPGSSTLAKAALSLEPHVETGDHGPLHLNLIRLGREVCRPRNPFCAECPLLTV
                     CPEGARNTKGKGRRPSTSFALPGYPRSRKNGGHA"
     gene            complement(2750..3013)
                     /locus_tag="VF357_00515"
     CDS             complement(2750..3013)
                     /locus_tag="VF357_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522655.1"
                     /translation="MSDPSFSGNERKLLRCLLRFYREIGPGATPGLKGLDEEAGLERW
                     EISEAVAVLRAKGLIEYWELQPAVRLTPEGLRAVQCLPEEGNA"
     gene            complement(3010..3315)
                     /locus_tag="VF357_00520"
     CDS             complement(3010..3315)
                     /locus_tag="VF357_00520"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX7522656.1"
                     /translation="MKATLIVLVVLLAMVAAFAVQNPGIIVVRFLAYSGYTSLLMVIV
                     AAFAAGVVSAGLVGLPGFFRRRSEARAAARRIRELEAEVGDRKKAAAPSPPAPGGTP"
     gene            complement(3338..4327)
                     /locus_tag="VF357_00525"
     CDS             complement(3338..4327)
                     /locus_tag="VF357_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7522657.1"
                     /translation="MPETTAGTPENRRLRLRTARFSIGSAITLCLLKFAVGIVSGSLG
                     VLASAFDNVADIFMSSVNLLSIRKAMDPADASHPYGHGKVETLATFFQGGMIALTGAG
                     VVWEAVRRLREGSAPEGIGIDVGILVMIISVVASWFISARIRKGGETTGSTALAADSL
                     HFRTDVYSGSGILFSLVLYKFTGWKWLDPGVALAVGVYIFSAAVPLLKAAMQDLTDHQ
                     LPAETVTRVTAIIEAHRPMVVDWHDLRTRRSGSEMQVDFHVVVCRDHSLEQAHRVADH
                     LEMEIREMLGNAHVVTHIDPCETECPGSHECGRVKESISRLHSSERDTKETVS"
     gene            4483..5121
                     /locus_tag="VF357_00530"
     CDS             4483..5121
                     /locus_tag="VF357_00530"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7522658.1"
                     /translation="MLKITEIFASVQGETSYSGYPFAFVRLTGCNLRCRYCDTTYAYD
                     GGEEFPLEEVVFRVTAFGLTRVCVTGGEPLLQEETSALVAALLDRGQDVLVETNGTLP
                     LSRLDPRAVKIMDVKGPSSGEDRRMLWENFRHLTERDEVKFVISSDEDYRYAKEVAAR
                     YRRERKWGFLLSPAFGFLAPERLAGWMVEDRLDARLQLQLHKLVWGPARRGV"
     gene            5126..5845
                     /gene="queC"
                     /locus_tag="VF357_00535"
     CDS             5126..5845
                     /gene="queC"
                     /locus_tag="VF357_00535"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX7522659.1"
                     /translation="MTTGGKPIGIALVSGGMDSFVMAEFCRRESDLALLHVNYGQRTE
                     AKELLCFHAVAEHLKVPASRRLVADIGYLKRIGGSALTDGGIDIPKADLGREGVPVTY
                     LPFRNAHLVAIAVSWAEVIGAKNIYIGAVAADSSGYPDCRPEFYEAMNETIRRGTKEG
                     SGIVVKAPFVHLLKKDIVLMGKSMGVPFEQSWSCYREGEKACGVCDSCALRLRAFAEA
                     GVLDPLPYETRPDYRRESPAS"
     gene            6007..6213
                     /locus_tag="VF357_00540"
     CDS             6007..6213
                     /locus_tag="VF357_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522660.1"
                     /translation="MANFYENKGDRNVTWEDAGGFNTHLLTELRNAMLSVEDGGKPAG
                     PVQAVVAEWIKRLPAFDFPDDEIG"
BASE COUNT         1028 a         2179 c         2143 g         1101 t
ORIGIN      
        1 aggaccttcc ggacgttccc cgcgatgtct ccgacggtgg cgttgacctg tgcgagggcg
       61 aggcgcagcg gcttgctcat accgccattc tcttcgaaag cggccgcagg gggaagcccc
      121 ggatctcgtg tggtaaaatc gcctctccca tggctccttc ctccgccccc ctctacggcc
      181 gcgactacat cctgctgaac gccgtgaatt tcctctattc cctgtactcg gtcatcttca
      241 tcttccttcc cgcttatatg taccgccttg gaatccgcga gggagcgatc ggcgtgctga
      301 tggcgacggg ggcgctcgtc tcggtcctcc tcaagcccgg gctcgggatg gtggtggggc
      361 ggggggcgcg gcggtctttc ctgtcgctcg gcgcgttcct ggccgccgca tcgacggttc
      421 cgtggctctt cgtgaccgcg ccgggggcgc acctgttcgt cattcgcgtg gcgcaggggg
      481 cgtccttctc catcttcacc gccgcgtcat acgcctacat cgccgcgtcc gcgccgccgg
      541 cgcgccgcgc ggaagcgctg gggatcttcg ggctctcctt cttcctcccc gtgtcggtgg
      601 gcgggtggct cggggagtgg gtgatcggcc gggcggggtt ccatggcctg ttcgccgcgg
      661 ggatcgggat cgcgttcctg gccgccctct tcccgctcgc gatgcgcgaa ccctccgtgc
      721 gggagcgccc gtcgatggca tccctgaaga tcttcctctc ccggcccttc ctcctgccca
      781 acacggcggg gtacctcttc ggggtggcgt acgggtcgat cttcaccttc ctccccgtct
      841 acctcgtcgt gcggggatcg gagtccctcg gggcgtttgt cttcgtctac gccctgtcgg
      901 tcatcgggac gcggatgttc gggcggaaaa tgctcgaccg gctgccgcgg gagtgggtct
      961 ccctttgcgc cctggtcctg atgggcgcgg ggaatctcct gatccctgcg gttcccggcg
     1021 gagtgggcat cgtcctggtg ggcgccgccg cgggggcggg acacggtctc ctcttcccgg
     1081 ccctgtcggc ccttgtgctc gaccgggtgg gggagggctc gtgcgcgatg gcgatggcga
     1141 tgttcactgc cgccttcgac atggggctcg tcaccggcgc ggccgccttc ggcttcgtgg
     1201 ccgaacggtt cgggtacgag gcgatgttcg tcacggccgc cgcggtggta ggcgcgggaa
     1261 ccgccgcctt tttcgtcctg gaccccgcgt tcaggaaagc agcgcgtccc gaacccgcgc
     1321 catgagctcc gggacgtcgg cctccgtctt tccctctgtc ggaatcgggt cgaacatctc
     1381 gaccacgacg cggcccggcc gaatgcggag cgagcccgga gggacgatat cgtggctccc
     1441 cgacacgcgg accgggacga tcggcaatcc cgccttaagc gcgatgtaga agccgccttt
     1501 cttcagcggc tgcagcttcc cgtccttgct gcgcgtccct tccacgtaga tcaggacgga
     1561 gttgttccca gcgagcgccg ccgccgcgtt cgaaagcgcg gttctggact gcatcgcgtt
     1621 ccgccggtcc accatgatct gcccgaggga caggagcacc cacccgaaga ccggaatctt
     1681 cgagagctcc ttcttgaaga cccaatggag cgggtgcggg atcgataccg ccaacgcgag
     1741 ggagtcgatg tggctcgcgt ggttcgacat gaagacgtac ggggcgctcc ggtccacgcg
     1801 ggagacgccg cgcgtttcga cggtgacgcc gaagacgggc aggagaatca tggcgtagct
     1861 cttcgtcacc cagcggaaga ttctcccctt gcggcttcgg ccgggaacca gcatgcccag
     1921 cgcgatcgcc ggcagcccga tccccagggt gaccaggccg agaaacaccc catggaaaac
     1981 ggtgcgggcc gtcacgcgtg ccccccgttc ttcctactgc gggggtaccc cggcagcgcg
     2041 aagctcgtac tggggcggcg ccccttccct ttcgtgttcc gcgccccctc cgggcacacc
     2101 gtcaggagag gacattccgc gcagaacggg ttgcggggcc ggcacacttc ccgcccgaga
     2161 cggatgaggt tgaggtgcag cggtccatgg tccccggttt ccacgtgcgg ctcgagagag
     2221 agggcggcct tcgcaagggt agacgagccg gggaccagcc cgagacgctt cgtcacccgc
     2281 aggacgtggg tgtccacggg gaaggcgggc atgccgaagg agaacaggag caggatgttg
     2341 gcggtcttca cgcccacccc ggggaaggag gtgaggtact ccctcgcctc gggaagcggc
     2401 atcccccgca ggaaatcgag cgagtagccg ccccgctcct ccctgatccg cgccagcgcg
     2461 ccgaggatcg acttcgcctt cgccttggcg agcccaccca cccggatcgc ctcctcgagc
     2521 tccgacggcc gcgcggcggc aagcgcgggg agcgagggaa accgtttcgc caggcgatcg
     2581 tacgcgcggt cgcggttggc gtccgtggtg ttctgcgaga ggatcgtcag gacgaggttg
     2641 cggaccgggt cggtctcgtg ggcacagata tcgggcctgc cgaaccgcct ggcgagcagg
     2701 ccggagacgt cgcgaagacg cgcggaaagc ccgcgggccg cccccgccat caggcgttcc
     2761 cttcttccgg aagacactga accgcccgca gcccctcggg cgtcaggcgc accgcgggct
     2821 gcagctccca atactcgatc agtcccttcg cgcgcagcac ggccaccgcc tcggagatct
     2881 cccaccgctc cagcccggcc tcctcgtcca gccctttcaa tcccggcgtg gcgccgggcc
     2941 cgatctcacg atagaaccgc agcaggcaac ggagcagttt ccgctcgttg ccggaaaagg
     3001 aaggatcgct cacggtgttc ccccgggcgc ggggggcgac ggcgcagcgg cttttttacg
     3061 atccccgacc tcggcctcga gctcccggat ccgtcgcgcc gcggcgcggg cctccgacct
     3121 gcgtcggaag aacccgggaa gccccacgag ccccgccgag accaccccgg cggcgaacgc
     3181 ggccacgatc accatcagca gcgacgtgta gccagaatag gccaggaacc ggacgacgat
     3241 gatgccgggg ttctgcacgg caaacgccgc gaccatcgcc agcaggacca caaggacgat
     3301 gagcgtcgcc ttcatttccc ttcctccttc gctgcgttca ggaaaccgtt tccttcgtgt
     3361 cccgctcgga gctgtgcagc ctggagatgc tttccttcac gcggccgcac tcgtgggaac
     3421 ccgggcactc ggtctcgcac gggtcgatgt gggtcaccac gtgggcgttt cccagcatct
     3481 cccggatctc catctcgagg tggtccgcca cccggtgggc ctgttccagc gaatggtcgc
     3541 ggcacaccac cacgtggaaa tcgacctgca tctccgaccc ggagcgccgc gtccgcagat
     3601 cgtgccagtc caccaccatc ggccggtgcg cctcgatgat cgccgtgacc cgcgtcaccg
     3661 tctccgccgg gagctggtgg tccgtcaggt cctgcatcgc tgctttcagc agcggcacgg
     3721 cggcggaaaa gatgtagacg cccaccgcca gcgcgacccc cgggtccagc cacttccatc
     3781 ccgtgaactt gtacagcacg agggaaaaga ggatcccgct cccggagtag acgtccgtcc
     3841 ggaaatggag cgagtcggcg gcaagggccg tagagcccgt ggtctcgccc cccttccgga
     3901 tccgggcgga gatgaaccag gaggcgacca cggagatgat catcacgagg atccccacgt
     3961 cgatgccgat cccctcgggg gcgcttccct cgcgcaggcg acgcaccgct tcccaaacca
     4021 cacccgcccc ggtgagggcg atcatcccgc cctggaagaa cgtggccagc gtctccacct
     4081 tcccatgccc gtacgggtgg ctcgcgtccg cggggtccat cgccttccgg atcgacagga
     4141 ggttgaccga cgacatgaag atgtccgcca cgttgtcgaa ggccgacgcg aggacgccga
     4201 gggagccgga gacgatcccc accgcgaact tgaggaggca gagggtaatg gcgctcccga
     4261 tggagaaacg ggccgtccga aggcgcagcc ggcgattctc cggagtaccg gccgtggttt
     4321 ccggcaagag ttcgtgtgtc ctttccccgg gcacggcagc gcccggcggg tggatacagg
     4381 gaattggaca ccgtttccgg cgattgcaag gatagcacat cctgccgggt cccatgcggc
     4441 gcgccggccg gggcctgcgc ggccacgtgc tacaatgttc aaatgctgaa gatcacggaa
     4501 atcttcgcca gcgtccaggg ggaaacctcg tattcggggt acccgttcgc cttcgtacgg
     4561 ctcaccgggt gcaacctccg ttgccggtac tgcgacacca cgtacgccta cgacggcggg
     4621 gaggagttcc cgctggaaga ggtcgtcttc cgggtcaccg ccttcgggct gacccgcgtg
     4681 tgcgtcaccg gaggggagcc gctgctccag gaggagacgt ccgccctcgt cgctgcgctg
     4741 ctcgatcgcg ggcaggacgt gctcgtggag acaaacggca cccttcccct ttccaggctc
     4801 gacccccggg cggtgaagat catggacgtg aagggccctt cctccggcga ggaccggagg
     4861 atgctgtggg aaaacttccg ccacctgacg gagcgggacg aggtgaagtt cgtcatctcc
     4921 tccgacgaag attacaggta cgcgaaggaa gtcgcggcac ggtaccgccg cgaaaggaaa
     4981 tggggcttcc tcctttctcc ggcgttcggg ttcctcgcgc ccgagcggct cgccgggtgg
     5041 atggtggagg accgcctcga cgcccggctg caactgcagc tccacaagct cgtttgggga
     5101 ccggcccgga gaggggtgtg aggaggtgac gacgggcggc aagccgatcg ggatcgccct
     5161 ggtcagcggc gggatggaca gcttcgtgat ggcggagttc tgcaggcggg agtcggacct
     5221 cgccctgctc cacgtcaatt acggacagcg caccgaggcg aaggagctct tatgcttcca
     5281 cgcggtcgcg gagcatttga aggtccccgc ctcccggcgt ctcgtcgcgg acatcgggta
     5341 cctgaagcgg atcgggggga gcgccctgac cgacggcggg atcgacatcc cgaaggcgga
     5401 ccttggacgg gagggggtcc ccgtcaccta cctcccgttc cggaacgcgc acctggtcgc
     5461 gatcgccgtt tcgtgggccg aggtgatcgg ggcgaagaac atctacatcg gcgccgtggc
     5521 cgccgactcc tccggctacc cggactgccg ccccgaattc tacgaggcga tgaacgaaac
     5581 gatccggcgg ggtacgaagg aagggagcgg catcgtcgtg aaggccccct tcgtccacct
     5641 gttgaagaag gacatcgtcc tgatgggaaa atcgatgggc gtccccttcg aacagtcctg
     5701 gtcgtgctac cgggaagggg agaaggcgtg cggcgtctgc gattcgtgcg cccttcggtt
     5761 gcgcgccttc gccgaggccg gggtcctcga ccccctgccg tacgagaccc ggcccgacta
     5821 ccgccgcgaa tctcccgcat cgtgatccct gccataaata aatacggaac atgggatttc
     5881 gtctgaacat ccctttccct gttgacaggc gggacgtccg ccgctacagt cttagtccca
     5941 aaaccccccg aacgggacgg atgaatcttt tccgttgccc gtccgtctta gatagcagga
     6001 gagaagatgg cgaatttcta tgaaaacaaa ggcgatcgca acgtcacttg ggaagacgcg
     6061 ggggggttca acacccatct gctgacagag ttgcgcaacg cgatgctctc cgtggaagac
     6121 ggtgggaagc cggcgggtcc cgtgcaggcc gtggtggccg agtggatcaa gcggctgccc
     6181 gctttcgatt tccccgacga cgaaatcggc taagcactaa aggcttcgat tcataaatac
     6241 ggttacatgt gaccgttggg gagtcggact ccgggctcgc gggggcttcc actccgctac
     6301 ggctcgttcc cgacatccct gtctccactt cgctccagac gccgccccga tacgagcttc
     6361 gcgccgccgg ggtacccccg gtgcaggaag aacggggggt actgggtggg gaaccccgat
     6421 ggccatggac ggccaagtgc cgcgggagcc a
//
LOCUS       DASVJL010000030         4998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_192113, whole genome shotgun sequence.
ACCESSION   DASVJL010000030 DASVJL010000000
VERSION     DASVJL010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192113"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /gene="proB"
                     /locus_tag="VF357_00545"
     CDS             complement(<1..1181)
                     /gene="proB"
                     /locus_tag="VF357_00545"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX7522661.1"
                     /translation="MSPERTAAKRGKADSRFPGIRRVVVKLGSGTVTDPEKGLREPTI
                     RALAAQLSSAWTESGVSFVVVTSGAIAAGRKKLGMAERPRTVALKQAAAAVGQTSLMR
                     AYERAFEKRGRHVAQLLLTHEDFEDRERYVNTRNTLLTLLSRGIVPIVNENDTVATEE
                     IRLEGGGGGANDHLATLVTQMIGADLLILLTDSDGLFTKDPHRHPDARRIPVVRDIDD
                     ESIRAAVGRHISAEGTGGMASKIQAARVLSASGVPVVIASGLSRRSVLDALAGKDAGT
                     LILPRAAGKLSSRKMWIAYARHSQGTVLVDDGARKVLLEGGKSLLPAGVIGAQGRFRP
                     GDVVSIADRRGRVFARGIARWGSEQVDRGKGKRSAEVRTLLGPETPAEVVHRDDLTIL
                     P"
     gene            complement(1171..2178)
                     /gene="obgE"
                     /locus_tag="VF357_00550"
     CDS             complement(1171..2178)
                     /gene="obgE"
                     /locus_tag="VF357_00550"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX7522662.1"
                     /translation="MHFIDEATITVRSGDGGRGCVSFRREKFVPRGGPDGGNGGNGGS
                     VIIEVSPSLSTLLDFRYRNLFKAARGQHGMGKNMHGKGAPDRRIAVPPGTIIADADTG
                     EILADLTRPGETLLVAKGGRGGRGNSAFTSSVNQAPDQAEPGRPGRERRLRLELKLIA
                     EIGLIGLPNAGKSTLLSRISRAHPKIADYPFTTLSPVLGVVSHRDEEIVVADLPGLIE
                     GAHRGVGLGHRFLRHAERTEGLIHLLDASRDSDEIVAAYQTIRDEMEKFGPELAARPT
                     LLVFTKMDLTGARENAERALAGIGHPAKEIHFISAATGEGIPPLLDGMLALRKKETHV
                     A"
     gene            complement(2191..2448)
                     /gene="rpmA"
                     /locus_tag="VF357_00555"
     CDS             complement(2191..2448)
                     /gene="rpmA"
                     /locus_tag="VF357_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX7522663.1"
                     /translation="MAHKKGVGSSRNGRDSQSKRLGVKRFGGEAVSAGCIIIRQRGTA
                     IHPGENVGIGKDHTLFALIDGVVQFARMGKDRKRVSVLSAS"
     gene            complement(2474..2788)
                     /gene="rplU"
                     /locus_tag="VF357_00560"
     CDS             complement(2474..2788)
                     /gene="rplU"
                     /locus_tag="VF357_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX7522664.1"
                     /translation="MYAVVRTGGKQLRVSPGDVVNVEKLLVEPGATVELTDVLMVSTD
                     QGTTVGTPTVEGAAVICTAVRDGKGKKITIYKYKRRKGFSKKQGHRQPFTTLSVTEIR
                     VG"
     gene            2967..3962
                     /locus_tag="VF357_00565"
     CDS             2967..3962
                     /locus_tag="VF357_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7522665.1"
                     /translation="MADVVIVGGGPAGILLAHHFREHGIRHKVLGRGKVGQSWRDMRP
                     GMVLLSPGVPGTDWTSLALERPIWSFPGVRRPFPTREDFLCYVEAFARDQHVEVTERT
                     EVVGARRTPDGFAVAVSGGVEIPCRFLVIATGSSSVPRYPEIPGIAGNRRVLHSGDFL
                     NCMAYDRKRVLVIGGANSAAELCIELAGTAHTTMCTRGPMRYFSETGELDHIRGSSES
                     VLKELFRFGILSLREADPVVSLSDGTAAFASGARESFDWIVCATGYRPRWIPVEGGTV
                     EVGEKGYPRISLVGESSVPGLYFSGSLAMFHSRCAFIHGFRNYGEKVFWDIADRL"
     gene            complement(3963..>4998)
                     /locus_tag="VF357_00570"
     CDS             complement(3963..>4998)
                     /locus_tag="VF357_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7522666.1"
                     /translation="GEEVPGNGAGSDLGDVPSKDGGAPAPERLPDLSDVVGQPMARRA
                     LEIAAAGCHAMLLVGPPGCGKTMLAERLPGILPDLSASEALDATRIYGASGEPPWPRP
                     LLRRPFRAPHPSITAAGLLGGGNPPRAGEVSFAHCGVLFLDEFSEFRAEVREALRQPV
                     ESGEIRISRSGHRYRFPCRFLLLAATNPCPCGNAGHPRKVCRCSPPLLDRFSRKFSGP
                     LLDRIDLAVSVLPVAIEAWSGEACGESSAAVRRRVDACRAVQEARYAGRVSRTNGTVR
                     SSTAELLRELTPEAAAFLIRAAERLSLSGRAIGKACRVARTIADLAAERLAGLPHAAE
                     ALQYRLSGFG"
BASE COUNT          745 a         1726 c         1690 g          837 t
ORIGIN      
        1 tgcggcagga tcgtcaggtc gtcgcggtgg acgacctcgg ccggggtctc cggcccgagg
       61 agggtgcgga cctccgcgct gcgctttccc tttccccggt ccacctgttc gctgccccac
      121 cgggcgatcc cccgcgcgaa aacgcgcccc cggcggtcgg caatggagac cacgtccccc
      181 ggacgaaagc gcccctgcgc cccgatcact cccgcgggca ggaggctctt ccccccctcc
      241 aggagcacct tccgcgcgcc gtcgtccacg agaacggtcc cttgggagtg gcgggcgtac
      301 gcgatccaca tcttgcggct tgaaagcttc ccggcggccc gcggcaggat gagtgtgccc
      361 gcgtccttcc ccgccagcgc gtcgaggacc gaccgccggg agagccccga ggcgatgacg
      421 accggtaccc cggacgccga caggaccctg gccgcctgga tcttcgatgc cattcccccg
      481 gttccttccg ccgagatatg ccggccgacg gcggcccgga tcgactcgtc gtcgatgtcc
      541 cgcacgaccg ggatgcggcg ggcgtcgggg tgccggtggg gatctttcgt gaagagaccg
      601 tcgctgtcgg tgagcaggat cagcaggtcc gccccgatca tctgggtcac cagcgtcgcc
      661 aggtggtcgt tcgctcctcc ccctcccccc tccaggcgga tctcctcggt ggccaccgtg
      721 tcgttctcgt tgacgatcgg gacgatcccc cgggagagca gggtgagaag cgtgttcctc
      781 gtgtttacgt accgctcccg gtcctcgaaa tcctcgtggg tgaggagcag ctgcgccacg
      841 tggcgcccgc gcttctcgaa cgcccgctcg taggcccgca tcagggaggt ctgccccacg
      901 gcggcggccg cctgcttgag cgccaccgtc cgggggcgct ccgccatccc cagcttcttc
      961 ctccccgcgg cgatcgcccc ggaggtgacc acgacgaagg aaactcccga ctccgtccat
     1021 gcggaggaga gttgcgcggc gagtgcgcgg atggtcggct cccgcagccc cttctcgggg
     1081 tcggtaaccg tgccgctccc cagtttgacg acgacgcgcc ggatcccggg gaaccgggaa
     1141 tcggccttcc cgcgcttcgc ggccgtccgc tcaggcgaca tgcgtctcct tctttctcag
     1201 ggccagcatc ccgtcgagca ggggagggat cccctccccg gtggccgcgg agatgaaatg
     1261 gatctccttc gcggggtgcc cgatccccgc cagcgcgcgc tccgcgtttt cacgggctcc
     1321 cgtcaggtcc atcttggtga agacgagcaa ggtgggacgt gccgcgagtt cgggaccgaa
     1381 cttctccatt tcgtcgcgga tggtctggta ggcagcgacg atttcgtcgg aatcccgcga
     1441 cgcgtcgagc aggtggatca gcccctccgt ccgttcggcg tgcctcagga agcggtgccc
     1501 cagccccacc ccccggtgcg cgccctcgat caggcccggc aggtccgcga cgacgatctc
     1561 ctcgtcccgg tgggagacca ctccgagaac gggggaaagg gtcgtgaacg ggtagtcagc
     1621 gatcttcggg tgtgcccgcg agatccgcga aaggagcgtc gatttcccgg cgttcggaag
     1681 gccgatcagc ccgatctcgg cgatcagctt gagctcgagc cgcagccgcc gctcccgccc
     1741 ggggcgcccg ggttccgcct ggtcgggggc ctggttcacg gaggaggtga acgcagaatt
     1801 gcctcggccc ccgcgccctc ccttcgcgac caggagcgtt tccccggggc gcgtgagatc
     1861 cgcgaggatc tcgccggtat ccgcgtcggc gatgatcgtc ccgggaggga cggcgatccg
     1921 tcggtcgggg gcgccctttc catgcatgtt ctttcccatg ccgtgctggc cgcgcgccgc
     1981 cttgaacagg ttccggtacc ggaagtcgag cagggtggag aggctcgggc tcacttcgat
     2041 gatgacgctt ccgccgttcc ccccgttgcc gccgtcgggg ccgccgcggg gaacgaactt
     2101 ttcccggcga aaactgacgc agccccgacc accgtccccg gaccggacgg tgatcgtggc
     2161 ttcatcgatg aagtgcatga acgggaggcg ctacgaggcg gagaggacgg aaaccctctt
     2221 gcgatctttt cccatgcggg cgaactgaac cacgccgtcg atcagggcga acagggtatg
     2281 gtccttcccg atcccgacgt tttctcccgg gtggatcgcg gtcccccgct ggcggatgat
     2341 gatgcacccg gcggagacgg cctcgccgcc gaaccgtttc acgccgagcc gcttgctctg
     2401 actgtcccgc ccgtttctcg aactgccgac gcctttttta tgcgccatgg tgtcctggcc
     2461 ccttctccgc gggtcaaccg acccggattt cggtcacgga aagcgtggtg aacggctggc
     2521 ggtgcccctg tttcttcgag aagcccttcc gcctcttgta cttgtagatg gtgatcttct
     2581 tccccttgcc gtccctgacc gccgtgcaga tcacggcggc gccctcgacg gtgggagttc
     2641 cgacggtggt tccctggtcg gtcgatacca tcaacacgtc cgtgagttct acggtcgcgc
     2701 cgggctcgac caaaagcttc tccacgttga cgacatcgcc cggggagacg cggagttgct
     2761 tcccccctgt gcggacaact gcgtacatag gatattgccc tccatgcggt tcgataagaa
     2821 caaactatta tagggaaaga ggtttcacct gtcaacttcg aaaatcgccc catgaaaccg
     2881 gccgggctgt ggaacaaccg gaagttgaag gagatatact acaacggatc aggacgcgag
     2941 ggaatgtcgg gggggaggca ggcatcgtgg cagacgtcgt catcgtcggg ggggggccgg
     3001 ccgggattct cctggcgcac cacttccggg agcatgggat tcgtcacaaa gtcctcggga
     3061 gggggaaggt tggacagtcg tggcgcgaca tgcgccccgg aatggttctt ctctccccgg
     3121 gggtccccgg caccgactgg acctccctgg ccctggagcg gccgatctgg tccttccccg
     3181 gcgtacggcg gccattcccg actcgcgagg atttcctgtg ctacgtggaa gccttcgcgc
     3241 gcgatcagca cgtcgaggtc accgagcgca ccgaggtcgt cggcgcgcgg cggacgccgg
     3301 atgggttcgc cgtcgcggtg agcgggggcg tcgagatccc ctgccggttt ctcgtgatcg
     3361 ccacgggaag ctcctccgtg ccccgctatc cggagattcc ggggatcgcc gggaaccggc
     3421 gcgtgctgca ctcgggcgat ttcctcaact gcatggcgta cgaccgcaag cgggtgctcg
     3481 tgatcggcgg cgcgaactcc gccgcggagc tttgcatcga gctcgccggg accgcgcata
     3541 cgacgatgtg cacccgcggt ccgatgcgct acttcagcga aacgggggag ctggatcata
     3601 tccgcggctc atcggagagc gtcctgaagg agttgttccg cttcgggatc ctctccctgc
     3661 gcgaggcgga cccggtcgta tccctgtcgg acgggacggc ggcattcgcc tccggggcgc
     3721 gggagtcgtt cgactggatc gtgtgcgcca cggggtatcg tccccgatgg atccccgtcg
     3781 agggcggcac agtcgaagtc ggggagaagg ggtacccccg catctccctg gtcggggagt
     3841 ccagcgtacc gggcctctac ttttccggat cgctcgcgat gttccactcc cgctgcgcct
     3901 tcatccacgg cttccgcaac tacggcgaaa aagtgttctg ggacatcgcc gaccggctgt
     3961 aatcacccga atccggacag gcggtactgg agggcctccg cggcgtgggg gagccctgcc
     4021 aagcgctcgg cggcgaggtc ggcaatcgtt cgcgccacgc ggcacgcctt cccgatcgcc
     4081 cttcccgaca gggagagccg ttccgccgcc cgtatgagga atgcggccgc ttccggcgtg
     4141 agctcccgta atagttccgc cgtcgaagag cggacggtcc cgttcgtccg ggaaacgcgc
     4201 cccgcgtacc gcgcctcctg aaccgcccgg caggcgtcga cccggcgccg gaccgccgcg
     4261 gacgattcgc cgcacgcctc gccggaccac gcctcgatcg cgaccggaag cacggagacc
     4321 gcaaggtcga tccggtcgag gaggggcccc gaaaatttcc tcgagaaccg atcgaggagc
     4381 ggcggggagc accggcagac cttccggggg tgacccgcgt tgccgcacgg gcacgggttg
     4441 gtcgccgcca gcagcaggaa ccggcacggg aaccggtacc ggtggcctga tcgcgagatc
     4501 cggatctctc ccgattcgac cggctgccgg agcgcctccc ggacctcggc gcggaattcg
     4561 gagaattcgt cgaggaaaag cacaccgcag tgagcgaagg agacctcgcc cgcccggggc
     4621 gggtttcctc cccccagcaa accggccgcg gtgatcgacg ggtgaggcgc ccggaacggc
     4681 cgtcggagga gagggcgggg ccagggcggt tccccggacg ctccgtagat gcgcgtcgcg
     4741 tcgagggcct ccgacgccga gaggtccggg aggattccgg gcaggcgttc ggcgagcatc
     4801 gtttttccgc atcccggcgg cccaacgagg agcatcgcgt ggcaccccgc cgccgcgatc
     4861 tcgagggccc ggcgcgccat cggttgcccg accacgtccg agagatcggg aaggcgctcg
     4921 ggagcggggg cgccgccgtc cttcgacggg acgtcaccga ggtcggagcc agcgccattg
     4981 ccgggaactt cctcgccc
//
LOCUS       DASVJL010000031         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_567380, whole genome shotgun sequence.
ACCESSION   DASVJL010000031 DASVJL010000000
VERSION     DASVJL010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567380"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VF357_00575"
     CDS             <1..630
                     /locus_tag="VF357_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7522667.1"
                     /translation="PSVSDTEIKPQHLVREIDRLSGGDAIVCSDVGQHQMWGAQFLRF
                     NRPRLWISSGGLGSMGFGLPSAIGAQIANPGRLVFALVGDGGFQMSIPELATVANNSL
                     PIKIVVMNNGYLGMVRQWQELFYNNRLSSVAITGFPDASMLAGAYGFPGRTVEDPGEI
                     GQALDDAVRHPGPYLLNVKVSPLECVYPIVPAGGAINEMVLGPTKTAAV"
     gene            667..1215
                     /gene="ilvN"
                     /locus_tag="VF357_00580"
     CDS             667..1215
                     /gene="ilvN"
                     /locus_tag="VF357_00580"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX7522668.1"
                     /translation="MLQTISLLVENKPGALMRITGVLSARGWNIDSLTVARTLDAAQS
                     RMTIVVDIEPVLRGQVIKQMNKLINVLQATDLTESLAVRREMALVRVRATPENRAGIL
                     RDAEIYGARVVDATAVGFGLECTGPVEKVEEFIDAMKTFGDIDVTRSGIVAVSMESRK
                     LRLAPPIEAAMGVNGPEQRPSC"
     gene            complement(1275..1739)
                     /locus_tag="VF357_00585"
     CDS             complement(1275..1739)
                     /locus_tag="VF357_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522669.1"
                     /translation="MSGCPGSRTMTFPKDDGTEVPSGKVPSQLRQWPIQLHLVSPAAP
                     YFQGADVVLAADCVPFAMGDFHNTYLKGKALAIACPKLDGDQEIYLEKIRGLFEDAKI
                     NSLTVTIMQVPCCRGLLGLAMQALKASSRKVPVKSVIVSLQGEVLQEEWTAA"
     gene            complement(1829..2266)
                     /gene="arfB"
                     /locus_tag="VF357_00590"
     CDS             complement(1829..2266)
                     /gene="arfB"
                     /locus_tag="VF357_00590"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX7522670.1"
                     /translation="MIFAGFRSGGKIVVDEGDIEERFIRSSGPGGQNVNKVATAVQLR
                     FDVRNSRSLTEGVRERLIRLAGKRVTGEGVLVIEARRFRTQERNRQDARDRLIQWLEK
                     AAAPVKPRRKTRPTAGSRERRLEGKQLRSETKRVRKPVSPSDD"
     gene            complement(2263..>2606)
                     /gene="cynS"
                     /locus_tag="VF357_00595"
     CDS             complement(2263..>2606)
                     /gene="cynS"
                     /locus_tag="VF357_00595"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: protein motif:HMM:TIGR00673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEX7522671.1"
                     /translation="ALFYGQAQATAAEARKIVTKLGLGRNRKEVISFLAAFPDKGLGP
                     VVPTDPLIYRLYEIMQVYGYPFKAIVHEKFGDGIMSAVDFTMDVEKEKDPKGDRIKVT
                     MSGKYLPYKIW"
BASE COUNT          446 a          843 c          842 g          475 t
ORIGIN      
        1 ccttccgtgt cggacaccga gatcaagccg cagcacctgg tgcgggagat cgaccgcctc
       61 tccggcggag acgcgatcgt ttgttccgac gtggggcagc accagatgtg gggagcgcag
      121 ttcctgcggt tcaatcggcc ccggctgtgg atcagctccg ggggtctcgg ctcgatgggg
      181 ttcgggctgc cttccgcgat cggcgcgcag atcgccaacc cgggccggct tgtcttcgcc
      241 ctggtggggg acggcggctt ccagatgtcg atccccgagc tggccacggt ggccaataac
      301 agcctgccga tcaagatcgt cgtgatgaac aacgggtacc tcgggatggt tcggcaatgg
      361 caggagctgt tctacaacaa ccggctctcc tccgtggcca tcacgggatt ccccgacgcg
      421 tcgatgctcg ccggggcgta cgggttcccc ggacgcacgg tggaggatcc gggggagatc
      481 gggcaggcgc tggacgacgc ggtccgccac ccgggcccgt acctgctcaa cgtcaaagtg
      541 tcgccgctcg agtgcgtcta cccgatcgtc ccggccggcg gggcgatcaa cgagatggtg
      601 ctggggccga cgaaaacggc ggcagtctag gatcatcggg aaatcccggg ggagggtcga
      661 cgaccgatgc tgcagacgat ttcgctgctg gtggagaaca agccgggggc gctgatgcga
      721 atcaccgggg tgttgagcgc gcgcgggtgg aacatcgaca gcctgacggt cgcgcgaacc
      781 ctcgacgcgg cgcaatcccg aatgacgatc gtggtggaca tcgaaccggt cctgcgcggg
      841 caggtcataa agcagatgaa caaactgatc aacgtgctcc aggccacgga cctgaccgag
      901 tcgctcgccg tccgacgcga gatggcgctg gtgcgtgtgc gggccacccc ggagaaccgc
      961 gcggggatcc tgagggacgc ggagatctat ggggcacggg tggtggacgc gaccgcggtc
     1021 ggcttcggac tggagtgcac cggcccggtc gagaaggtcg aggagttcat cgacgcgatg
     1081 aagaccttcg gggacatcga cgtcacccga tccggcatcg tggccgtgtc gatggagagc
     1141 cggaagctcc ggctggcccc cccgatcgag gcggcgatgg gagtcaacgg ccccgagcaa
     1201 cggcccagct gctgatccgg gacctttccc atcattatcg tattacgagg cgtccgcagt
     1261 aatgaattga agcgctacgc cgcagtccat tcctcctgca gcacctcgcc ctgcaggctc
     1321 acgatcacgg acttgaccgg gacctttcgc gaactcgcct tcagtgcctg catcgccagc
     1381 ccgagcaacc cgcggcagca cgggacctgc atgatcgtca ccgtcagcga gttgatcttc
     1441 gcgtcctcga acagaccgcg gatcttttcg aggtatatct cctggtcccc atcgagcttc
     1501 gggcaggcga tcgcgagggc cttccccttc aggtacgtgt tgtgaaaatc ccccatggcg
     1561 aaggggacgc agtccgccgc cagcaccacg tccgccccct gaaaatacgg ggccgccggt
     1621 gagaccaggt ggagctggat cggccactgg cgaagctggg agggaacctt ccccgacgga
     1681 acctccgtcc cgtcgtcctt cggaaacgtc atcgtcctcg aacccgggca accgctcatc
     1741 gacccacctc cggaaagata tcgtcttaag gaggattatc cgcgaggaga tccttgccgt
     1801 ccttgacgca ggtcaaccgg gtgatccgtc aatcgtcgga aggcgaaacg ggtttgcgca
     1861 cccgcttcgt ttcgctccgc agctgcttcc cctccagccg gcgctccctc gatcccgcgg
     1921 tcggccgggt ctttcttcgg ggcttgacgg gtgcggcggc tttttccagc cattggatca
     1981 agcggtccct cgcgtcctgg cggttccgtt cctgtgtgcg gaaacggcgg gcctcgatga
     2041 ccagcacgcc ctcgcccgtc acccgcttcc cggcaagccg gatcagccgt tcgcgcaccc
     2101 cttcggtcag ggaccgggag ttccgcacgt cgaagcgcaa ctggaccgcc gtcgcgacct
     2161 tgttgacgtt ctgcccgccg ggcccggagg agcggatgaa ccgctcctcg atgtcgccct
     2221 cgtcgacgac gatcttccct ccggatcgga atccggcgaa aatcaccaga tcttgtacgg
     2281 caggtatttc cccgacatgg tcaccttgat gcggtcgccc ttcgggtctt tctctttttc
     2341 cacgtccatc gtgaagtcga cggcgctcat gatgccgtcg ccgaatttct cgtggacgat
     2401 cgccttgaag ggatagccgt acacctgcat gatttcgtac aggcggtaga tgagtgggtc
     2461 cgtgggaacg accgggccca gccccttgtc ggggaaagca gccaggaacg atatgacctc
     2521 cttccggttt cttcccagcc caagcttggt gacgattttc ctggcttcgg cggcagttgc
     2581 ctgcgcctga ccgtagaaca gggccg
//
LOCUS       DASVJL010000032         3837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1036617, whole genome shotgun sequence.
ACCESSION   DASVJL010000032 DASVJL010000000
VERSION     DASVJL010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036617"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VF357_00600"
     CDS             <1..632
                     /locus_tag="VF357_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX7522672.1"
                     /translation="SYYLIMGDKLPMEKVRPVPGAASKGDPQEVMGRDGEDLVRRTLP
                     VSGMTCASCVGRVERALRKVPGVIAASVNLAVQTASVEYLPRVASVADLRAAIEGAGY
                     SVPEVSPGEDPVARQDRTQREEERALLTRLRVGVVLGVPLLLFSHWEMFAGHGTLPFS
                     PFNAALLQFLLATPIQFYVGARFYRGAWVVARHGSTDMNTLVALGTSVA"
     gene            738..2366
                     /locus_tag="VF357_00605"
     CDS             738..2366
                     /locus_tag="VF357_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEX7522673.1"
                     /translation="MGRYFESRARGKTSEAVKKLIGLAPKTARVVRDGAEVDVPLESV
                     VVGERVVVRPGEKVPVDGTVVEGRSAVDESMLTGEPLPVEKVPGSGVTGGAMSLDGRL
                     LFTATRVGKDTVLSRIVAIVREAQGSKPPIGRLADVIASYFVPAVMAAALLTFVAWYF
                     LGPEPRGTYAMVNMISVLIIACPCAMGLATPTSIMVATGRGAELGILVRDGAALETAQ
                     KVDTIVFDKTGTVTKGKPELGEVRLVPGGAFRGEEGMRELLRLAASAESGSGHPLADA
                     VVRGARGRGIDVAAPETFVSVPGQGIRASVSGREIRVGNLAWLEGEGIDPATIGHDVQ
                     EIADAGGSPICVAVDGKGEGVLSVADEVKDGAPEAIRRLREMGLDVVMLTGDNRRTAK
                     AVAEKVGISRVIAEVLPDRKAHEIRTLQAAGRVVAMAGDGINDAPALAQADVGIAMGT
                     GADIAVEAGDLVLMGGELRGVVDAISLSRATLRNIRQNLFWAFAYNVVLIPVAAGVLY
                     PSFRLLLNPVYAAAAMGLSSVTVVANALRLRRFR"
     gene            2380..3564
                     /gene="rnd"
                     /locus_tag="VF357_00610"
     CDS             2380..3564
                     /gene="rnd"
                     /locus_tag="VF357_00610"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX7522674.1"
                     /translation="MEKEPMGRVVTDPAEFRDLVAAMRASGKVGFDTEFVGERTYFPR
                     LCLIQLGTETMSVAVDPLAVGDLSPLDDLLFDASVLKLVHAGWQDLKIIHQRTGRVPA
                     PVFDTQIAAALLGLPAQAAYASVVHAFLEVEVKKGHSCSDWAARPLSPSQLAYALDDV
                     RYLPALHDRMVERLGKEGRLSWIEPEIAALSSPASYETDPLQEYRRVKGWAALDRRRL
                     AVLRALIAWREGEARRRDVPRRRVLADESALAIARSKPANEEALRRVRGLEWKSGGGS
                     SSAVLAMVRDALALPDSLLPEAPPSRPRGNGDRASVVGLLGALVRSRSRVHRVAPEVL
                     TNTDELERLAAGERGGIGVLSGWRLDLLGRELLALLEGRISLVIHRGEVVAEERDATA
                     KR"
     gene            3576..>3837
                     /locus_tag="VF357_00615"
     CDS             3576..>3837
                     /locus_tag="VF357_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX7522675.1"
                     /translation="MRKNASLLVAVLLTGGVLFTQGGCAAGVSAGKEAGAVQSSEASA
                     LLPSVIAGVPFLPQEDDTCGPSSLAMVLGFLGQDVNTQEIVRE"
BASE COUNT          538 a         1206 c         1457 g          636 t
ORIGIN      
        1 catcctatta tctgatcatg ggagacaaac taccgatgga aaaggtgcgc ccggtcccgg
       61 gtgccgcctc caagggtgat ccgcaggagg ttatgggacg ggacggcgag gatcttgtgc
      121 ggaggactct ccccgtttcc gggatgacgt gcgcgtcgtg cgtggggcgg gtcgagcggg
      181 cgcttcggaa ggtcccaggg gttatcgcgg cgtccgtcaa cctggcggtg cagacggcga
      241 gcgtggagta cctgccccgg gtcgcctccg tggccgacct gcgggccgcc atcgaggggg
      301 cggggtattc ggtcccggaa gtgtcgccgg gggaggaccc ggtcgcccgg caggaccgga
      361 cgcagcggga ggaggagcgg gccctcctga cgcggttgcg cgtcggtgtt gtcctgggtg
      421 ttcccctcct ccttttctct cattgggaga tgttcgcggg gcacggcacc ttgccgtttt
      481 cgcccttcaa cgcggcgctg ctgcagttcc tccttgccac gccgatccag ttctacgtcg
      541 gtgcgcgctt ctaccgaggc gcgtgggtcg tcgcccggca cggttccacc gacatgaaca
      601 cgctggtagc gcttggcacc tcggtggcgt aactctacag cttcgttgcc gccttctttc
      661 cttccctggt caccgccgag gggctcgtcg tccacctgta cttcgagacg tccgcggcga
      721 tcatcgtcct catcctcctg ggccgctatt tcgagtcccg ggcgcggggg aagacgtccg
      781 aggcggtgaa gaagctgatc ggcctggcgc cgaagaccgc ccgggtcgtg cgcgacgggg
      841 ccgaggtgga cgtccccctc gaatcggtcg tcgtcgggga gcgcgtggtg gtccggccgg
      901 gggagaaggt gccggtcgac gggacggtcg tggaaggccg ctccgcggtc gacgagtcga
      961 tgctcaccgg ggagccgctc ccggtcgaga aggtgcccgg cagcggcgtg accggcgggg
     1021 cgatgagcct cgacgggcgg ctcctcttca ctgcgacccg cgtggggaag gacaccgttc
     1081 tctcgcggat cgtcgcgatc gtgcgggagg cgcaggggag caagccgccg atcgggcggc
     1141 tcgcggacgt gatcgcgtcg tacttcgtcc ccgcggtgat ggcggcggcg ctcctgacgt
     1201 tcgtcgcgtg gtacttcctc ggacccgagc ctcgagggac gtacgcgatg gtcaacatga
     1261 tctccgtact gatcatcgcc tgcccgtgcg cgatgggact cgccaccccc acctcgatca
     1321 tggtcgccac cgggaggggg gcggagctcg ggatcctcgt gcgcgacggc gccgcgctgg
     1381 agaccgcgca gaaggtggac acgatcgtct tcgacaagac cggcacggtg acgaagggga
     1441 agccggagct gggcgaggtg cggcttgtcc cgggaggcgc gttccgggga gaagagggga
     1501 tgcgggagct tctccgactc gcggcctccg cggagagcgg ctccgggcac ccgctggccg
     1561 atgcggtggt gcgaggggcg aggggtcggg ggatcgacgt cgcggcgccg gaaacgttcg
     1621 tttccgttcc gggacagggg atccgggcat cggtttccgg acgtgagatc cgcgtgggga
     1681 acctcgcctg gctcgaaggg gaggggatcg acccggcgac gatcggtcac gacgtgcagg
     1741 agatcgccga cgcgggcggc agcccgattt gcgtagcggt ggacggcaag ggggagggcg
     1801 tcctctccgt ggccgacgag gtgaaggacg gcgcgccgga ggcgatccgt cgcctgcgtg
     1861 agatgggcct ggacgtcgtc atgctcacgg gggacaaccg gcgcaccgcg aaggccgtcg
     1921 cggaaaaggt cgggatctcc cgggtgatcg cggaggtcct cccggaccgg aaggcgcacg
     1981 agatccgcac gctccaggcg gcgggaaggg tggtcgcgat ggcgggggac gggatcaacg
     2041 acgccccggc gcttgcgcag gccgacgtcg gcatcgccat ggggaccggc gcggacatcg
     2101 ccgtggaggc gggggacctg gtgctgatgg gaggggagct gcggggggtc gttgacgcca
     2161 tctccttaag ccgcgccacc ttgcgcaata tccggcagaa cctgttctgg gcgttcgcct
     2221 ataacgtggt actcattccg gtcgccgcgg gagtactcta tccttcgttc cggctgttgc
     2281 tgaacccggt gtacgccgcc gccgcgatgg ggctctcctc ggtgacggtc gtcgccaacg
     2341 ccctgcgcct gcgtcgattc cgctaggaaa agagggggaa tggagaagga accgatgggg
     2401 cgggtcgtga cggacccggc ggagttccgc gacctggtcg cggcgatgcg cgcatccgga
     2461 aaagtcgggt tcgacaccga gttcgtgggg gagcggacct acttcccccg gctctgcctg
     2521 atccagctcg gcacggagac gatgtccgtc gcggtagacc ccctcgccgt cggcgatctg
     2581 tcgccgctcg acgacctgct gttcgacgcc tcggtcctca agctggtgca cgccgggtgg
     2641 caggatctga agatcatcca ccagcggacc gggcgggtcc ccgcgccggt tttcgacacg
     2701 cagatcgccg cagcgctcct cgggcttccc gcccaggcgg cgtatgcctc ggtagttcat
     2761 gcgttcctcg aggtcgaggt gaagaagggg cactcctgct ccgactgggc cgcccgtccg
     2821 ctctccccgt cgcaactcgc gtatgcactg gacgacgtcc ggtatctccc cgcgctgcac
     2881 gaccgtatgg tcgaacggct ggggaaggaa gggcgcctct cgtggatcga acccgaaatc
     2941 gccgcattgt cctcgcccgc ctcctatgag acggatcccc tgcaggagta ccgccgcgtc
     3001 aaggggtggg cggccctgga ccgtcgtcgc ctggcggtgc tccgggccct gatcgcgtgg
     3061 cgggaggggg aggcgaggcg ccgggatgtc ccccgccgtc gcgtgctggc cgacgagagc
     3121 gcgctggcga tcgcccgcag caagcccgcg aacgaggagg cgctgcgtcg cgtgcgcggg
     3181 ctggagtgga agagcggggg cggctcgtcg tcggccgtcc tggcgatggt gcgggacgcc
     3241 cttgccttgc cggattcgct actcccggag gcccctcctt cacggccccg cggcaacggg
     3301 gaccgcgcct ccgtcgtggg gctcctgggc gcgctcgtgc ggtcccggtc gcgagtgcac
     3361 cgggtcgcgc cggaagtact gacgaatacc gacgaactgg agcggctggc ggccggggag
     3421 cggggaggga tcggggtgct gtcggggtgg cggctcgatc tcctggggag ggaattgctg
     3481 gccctgctcg aggggcggat ctccctcgtg atccatcggg gagaagtggt cgccgaggag
     3541 agggacgcca cggcgaagcg gtgaagggat cctcgatgcg gaaaaatgca tcgcttctgg
     3601 ttgctgtctt gctgacgggc ggcgtcctct tcacgcaggg aggatgcgcg gcgggcgtga
     3661 gcgcggggaa ggaggccgga gccgtccaat cctcggaagc gtcggccttg ctcccctcgg
     3721 tcatcgcagg ggtaccgttc cttccccagg aagacgatac ctgcggtcct tcctccctcg
     3781 cgatggttct cgggttcctg gggcaggatg tcaacacgca ggaaatcgtc cgggaga
//
LOCUS       DASVJL010000033         4653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_286189, whole genome shotgun sequence.
ACCESSION   DASVJL010000033 DASVJL010000000
VERSION     DASVJL010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286189"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VF357_00620"
     CDS             complement(<1..632)
                     /locus_tag="VF357_00620"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522676.1"
                     /translation="MPESHGLRSLPSVASLLASNAARAFLGSHPRAVVVDALREVVAA
                     ARVAVGEERVSLSREAWTDRILSMLPGEIASRESLPMHRVINAAGIVVHTNLGRAPLP
                     GEALAAVLETARGYSNLEFDLAAGERSSRLSHVEGMLRALTGAECAHVVNNNAAAVLL
                     CLAGHARGREVIVSRGELVEIGGSFRIPDIMAESGARLVEVGTTNRTRLS"
     gene            complement(646..1302)
                     /locus_tag="VF357_00625"
     CDS             complement(646..1302)
                     /locus_tag="VF357_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7522677.1"
                     /translation="MNIRIDNHMTLRERIVDTIRNAIVNGQLAPGTRIAEPELADKFG
                     ISRTPIREAFRQLESEGFITVVPRKGAIVASLSPRDIADFYDLKMVLEGYAARCAATT
                     LTESDLTKMETVNRQIEAASEKKDLRRLLDLHNEFHDIFLRSCGNEKLHTLVQNLVMQ
                     FRRFRLILAMPGRIEGSIRQHWEIIEAFRKRDPELAEELVRKNALYGKKILLRELAKA
                     "
     gene            1502..2290
                     /locus_tag="VF357_00630"
     CDS             1502..2290
                     /locus_tag="VF357_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX7522678.1"
                     /translation="MPWVVHSTVPYPLLKEPGTPRMLSDAGVGPEIYLSGATLDAITP
                     IEAETVAETLRNAGIRSLSFHAPFEDVWPGARDEEARRFAVRRLSGAIALAPLFRPAG
                     IVLHGGYYDWLFDFQPEKWFAPARRSFGELAEAAEKAGTDLFVENVFDEIPDHLLRLR
                     EAVGSSRLHFCFDPGHATLFSRLPVQKWAEAFGEGIRLMHVHDNRGRRDDHLPVGEGG
                     INFRGVLLAVLDAGARPILTVEPHRKDHFARSVAGLHAILATIP"
     gene            complement(2566..2922)
                     /locus_tag="VF357_00635"
     CDS             complement(2566..2922)
                     /locus_tag="VF357_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7522679.1"
                     /translation="MSPHKTIRIREEGDTLVVCVGGYLNSLLGEEVEKVVSAKLDDGG
                     RRILLNFQGTRLVNSIGISFVIGVVEKVMEQEGRMAFCELSRINCDLFRVTGLAKYVR
                     SFETEKEALDYLAGNA"
     gene            complement(2922..>4653)
                     /locus_tag="VF357_00640"
     CDS             complement(2922..>4653)
                     /locus_tag="VF357_00640"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7522680.1"
                     /translation="IKAGHFPMFLSGSSPGRVTAALKREGLFGTFQMIEVGGLSTDSS
                     VRLWGHLCERRRIDIPASLLPRASGRLGGIPAYQRMLVEEIFFRSEKVPDAVTLENLY
                     AVSVTEGKLNRYWKEFFENTFPDRAQRGRAIRFLKRVLCDRFPLDTVEGAISLMGVSE
                     GEGEAILSALEFKGLLKADLDQLTFVGDPVLADFLYWAFERGVLGKGTSQVAAAIVQA
                     RLSHTAPEPGQGGDHARRVAVVKELMRKWDLREVPLLLLDFKMFREKFGGKGLLEVVI
                     GMEHEAVRVRLPKVSSVSTGYRASRGGPRFDFDLVAYGFLDSDFSEENLVIWAVDAAV
                     EKNLGARAVEHFENRCRLLALEKRLPHDRLRKWMIINETADPAAVDLASRYGIHLSHP
                     TQLRLFLNLFGLEELEREPEPVRTPGAEAPRVGKTLEYELVLPIKADSEVVAARVAEE
                     VAAFAAVDADTVDRIKMAIIEACINAFEHSASESGKVRLRYLLSPDKIELFVQDDGKG
                     FRSGKTTEESKKNRGWGLKLIRELVDDVEVITGPDGTVVHMVTHLGGDPANPGTVQQG
                     EGGSAPPGER"
BASE COUNT          766 a         1587 c         1493 g          807 t
ORIGIN      
        1 tcggaaaggc gcgtccggtt tgtggtcccc acctctacca ggcgggcgcc gctctccgcc
       61 atgatgtcgg ggatccggaa cgatccgccg atctcgacga gctcgccgcg gctgacgatc
      121 acttcccgcc cccgggcgtg gcccgcgaga cagaggagca ccgcggcggc gttgttgttg
      181 acgacgtggg cgcattcggc gccggtcagg gcgcgaagca tcccctcgac gtgcgagagg
      241 cgggatgatc gttcccccgc cgcgaggtcg aactcgaggt tcgaataccc ccgtgcggtt
      301 tcgaggaccg cggcaagcgc ctctccgggc aacggcgcac gcccgaggtt ggtgtgaacg
      361 acgatcccgg cggcgttgat cacacggtgc atcgggaggg actcccttga cgcgatctct
      421 cccgggagca tcgaaagaat ccggtcggtc cacgcctcgc gcgagagcga aacccgttcc
      481 tcgccgacgg cgacgcgggc ggcggcgacg acctcgcgga gcgcgtccac caccacggcg
      541 cggggatggg atcccaggaa cgcgcgggcg gcgttgcttg ccagcaggga ggcgacggac
      601 ggaaggctgc ggagtccgtg ggattcgggc atcgccgacg gcggttcagg ccttcgccaa
      661 ttccctgaga aggattttct tgccgtagag ggcgttcttg cgcacgagct cctcggcgag
      721 ctccgggtcg cgcttgcgga acgcctcgat gatctcccaa tgctgccgaa tcgacccctc
      781 gattcttccc ggcatggcga ggatcaggcg gaagcgccgg aactgcatga ccaggttctg
      841 cacgagcgtg tggagcttct cgttcccgca cgagcgcagg aagatgtcgt ggaactcgtt
      901 gtgaaggtcg agcaggcggc gaaggtcctt cttctccgac gcggcctcta tctggcggtt
      961 gaccgtctcc atcttcgtca ggtcggactc cgtgagcgtc gtggccgcgc accgagccgc
     1021 gtacccctcg aggaccatct tcaggtcgta gaagtcggcg atgtcccgcg gcgacaggga
     1081 ggcgacgatg gcccccttgc gcggcaccac ggtgatgaat ccttccgact ccaactgacg
     1141 gaacgcctcc cggatcggcg tccggctgat cccgaacttg tcggccagtt ccggttccgc
     1201 gatgcgggta ccgggggcca gttggccgtt gacgatggcg tttcgaatcg tatcaacgat
     1261 ccgttcacga agggtcatgt ggttgtcgat ccggatattc atttttttca atggcttacc
     1321 ccttgctccg aaaaataaac agcattttat gtatacagta tgcataaaaa atgtcaaccc
     1381 tgaaaattgc accggaaatc gcccccccct tcaggggtgc gtcggagggc gtccacttgc
     1441 gcggggaacg gggataagct aatttcgttt tttctccgga aaatccgccg ggaggaatgc
     1501 catgccctgg gtcgtccact ccaccgtccc ctaccccttg ctgaaggagc caggcacgcc
     1561 gcggatgctg tccgacgccg gcgtcgggcc ggagatctac ctatcggggg cgaccctcga
     1621 cgcgatcacc ccgatcgagg cggaaaccgt cgcggagacg ttgcgaaacg cgggaatccg
     1681 ctccctgtcg ttccacgccc cctttgagga cgtgtggccc ggcgcgcgcg acgaggaggc
     1741 gcggcggttc gccgtgcgcc ggctctccgg cgcgatcgca ctcgccccgc tcttccgccc
     1801 cgccggaatc gttctccacg gcgggtatta cgactggctc ttcgacttcc agccggaaaa
     1861 gtggttcgcc ccggcccggc gctcgttcgg agaactcgcc gaagcggcgg agaaggcggg
     1921 aaccgacctg ttcgttgaga acgtcttcga cgagatcccc gaccacctgc tccgcctgcg
     1981 ggaggcggtg ggctcttccc gcctccactt ctgcttcgac ccgggccacg cgacgctctt
     2041 ctcccgtctg ccggtccaga agtgggcgga ggcgttcggg gaggggatcc gtctgatgca
     2101 cgtgcacgac aaccggggac gtcgggacga tcaccttccg gtgggcgagg gggggatcaa
     2161 cttccggggg gtgctgctgg cagtgctgga cgcgggggcc aggccgatcc tcacggtgga
     2221 accgcaccgc aaggaccact tcgcgcgcag cgtggcgggg cttcacgcga tcctcgcgac
     2281 gatcccctag gtgctccatt tcatcgatac ggtaacgcaa acggatgcgc tttgatctcc
     2341 ggggttccgg gacgcagggc gccccagtac gagctccgcg ctgctggggt acccccgcag
     2401 taggaaaaac gggggacaca gcgggggccg ctcccgccat ccatggctcc cgcggcactt
     2461 gcccgtccat gggcatcgac gatggtagcg gagtggaagc cccccgcgag accggagtcc
     2521 taactcctca acggccactt gttaccgtat ttaggagctg gagcactaag cgttcccggc
     2581 gaggtagtcg agcgcctcct tttccgtctc gaaggaacga acgtatttcg ccaggcccgt
     2641 tacgcggaaa aggtcgcagt tgatccggct caactcgcag aaggccatcc ggccttcctg
     2701 ctccatcacc ttttcgacga ctccgatgac gaacgagatc ccgatgctgt tgaccagcct
     2761 cgtaccctgg aagttcagca ggatccggcg gccaccgtcg tcgagcttcg cgctgaccac
     2821 cttctcgacc tcctcgccca gcaggctgtt caggtacccg ccgacgcaca ccaccagggt
     2881 gtctccctcc tcgcggatcc ggatcgtctt gtgcggagac atcagcgctc cccggggggt
     2941 gcggatcccc cctccccttg ctggacggtc ccgggattcg cgggatctcc ccccagatgc
     3001 gtcaccatgt ggacgacggt gccgtccggt cccgtgatga cctcaacgtc gtcgaccagc
     3061 tcccggatca gcttcagtcc ccatccccgg ttcttcttcg actcctccgt cgtcttcccc
     3121 gaccggaacc ccttgccgtc gtcctgcacg aagagttcga tcttgtcggg ggagagcagg
     3181 taccgtagtc ggaccttccc cgactcggag gcgctgtgct cgaaggcgtt gatgcacgcc
     3241 tcgatgatcg ccatcttgat gcggtccacg gtgtcggcgt cgacggcggc gaaagccgcc
     3301 acctcttccg cgacccgcgc cgcgaccacc tcggagtcgg ccttgatcgg caggaccagt
     3361 tcgtactcca gcgtcttccc gacgcgcggc gcctcggcgc cgggggtgcg gacgggctcg
     3421 ggctcccgct ccagttcctc gagcccgaac aggttgagga agagccgcag ctgcgtcggg
     3481 tgggacaggt ggatgccgta ccgcgacgcc aggtcgaccg ccgcagggtc cgccgtctcg
     3541 ttgatgatca tccacttccg gagccggtcg tgcggcagcc tcttctcgag cgccagcagc
     3601 cggcaccggt tttcgaaatg ctcgacggcg cgagcgccga ggttcttctc gaccgccgcg
     3661 tcgaccgccc agatgacgag gttctcctcg gagaagtcgc tgtcgagaaa cccgtacgcg
     3721 accaggtcga agtcgaagcg cggacccccc cgactcgcgc ggtaccccgt cgagacggag
     3781 gagaccttcg gcagccggac ccgcaccgcc tcgtgctcca tcccgatgac gacctcgagc
     3841 agccccttcc ccccgaactt ttcacggaac atcttgaaat cgaggagcag gagcggcacc
     3901 tccctcaggt cccacttccg catcaactcc ttcacgaccg cgacgcgtcg cgcgtggtcg
     3961 cccccctgtc ccggctccgg cgccgtgtgg gagagccgcg cctgcacgat cgccgccgcc
     4021 acctgcgacg tccccttccc gaggacgccg cgctcgaacg cccagtacag gaaatcggcg
     4081 agcaccgggt cccctacgaa cgtgagctga tcgaggtcgg ccttcaggag ccccttgaac
     4141 tcgagggcgg agaggatcgc ttccccctcc ccttcggaaa cccccatcag ggagatcgca
     4201 ccctccaccg tgtcaagcgg gaaccggtcg cacaagacgc gcttcaggaa ccggattgcc
     4261 cgccctcgtt gcgcgcggtc cgggaaggtg ttctcgaaga actccttcca gtaccggttg
     4321 agcttccctt ccgtgacgga gactgcgtac aggttctcga gggtgaccgc gtcggggacc
     4381 ttctcgctcc ggaagaagat ctcctccacg agcatccgct ggtaggcggg gatcccgccg
     4441 agccgcccgg aggcgcgggg aaggagggac gccggaatat cgatacggcg ccgctcgcac
     4501 aggtgccccc acaggcggac ggaggagtcg gtcgagagcc cgcccacttc gatcatctgg
     4561 aaggtgccga agagcccctc gcgcttgagg gccgcggtga cgcggccggg cgaggagccc
     4621 gagaggaaca tcgggaagtg gccggccttg atg
//
LOCUS       DASVJL010000034         3281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1224515, whole genome shotgun sequence.
ACCESSION   DASVJL010000034 DASVJL010000000
VERSION     DASVJL010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224515"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1871
                     /locus_tag="VF357_00645"
     CDS             120..1871
                     /locus_tag="VF357_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7522681.1"
                     /translation="MREFRPDLILEATNVGILCADRQGRILYANDAAAVQLGARKQEL
                     IGRAIDAVSKGAGDAFLEIVRTGKPQAGVKIRTSGGFVIADRNPVFDGGKVIGVVSVF
                     KDISRYEETATELQAYRELAKQLDAVIHSSYDGLYITNGNADTLFCNKAYLRVSGLTA
                     KDVEGKNMREIVRHGTINQSVTLEVLEKRRRVTIMQEFSNGRTAIVTGNPVFDDDGKI
                     ILVVSNVRDITELNELREQVQENRTLAKRYREELKKASLAGVNRDTVVFRSPAMENCI
                     LLAARASDVLSPVLLTGESGVGKGMLARLIHNLGSRKKGPFIHVNCGAIPAGLMESEF
                     FGYEKGAFTGASEEGKPGLFEMADRGTLFLDEIGEIPLPLQVKLLKVLEESEVRRVGG
                     TQPRKLDVRIVAATNRDLQEMTRNRLFREDLFFRLDVFPIRIPPLRERPEDIPPLIDR
                     TLGELNRRYKKGKRVRPDTLDLLVRHPFPGNVRELQNVVERSFILADGKWIVPEHLPT
                     GIRKSSTPPESGTVAHISRGKEGLEQILAGVEREILERLLLECGTTYAMADRLKVNQS
                     TVVRKLRKLGIRAHPAG"
     gene            2038..2367
                     /locus_tag="VF357_00650"
     CDS             2038..2367
                     /locus_tag="VF357_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522682.1"
                     /translation="MGEADVKGARTAGRQDDGGEPDAVFSRGSGVAYRYALRLRDPEE
                     AGTILQILADVAIPLLYLLLLPRDHRSGYRAYIGLASPDVLDLPIRFASRGIMIERGE
                     ETGDRRR"
     gene            2505..>3281
                     /locus_tag="VF357_00655"
     CDS             2505..>3281
                     /locus_tag="VF357_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7522683.1"
                     /translation="MRKQGRKWQAILVIGLVLVMGLADVALAAEKIVIGGMYPMTGRG
                     GRYGLDSVAAAEIAAEEINAKGGVNGRMIDLIFTDDKSNTSYGVKVANRYIKEDKVNF
                     LMGVVSSAVGLAVTEVSKENKIIFVGTDHASTALTMEKFQPYYFRVSNNTYQSAAAAA
                     LYATEKKEWKKYYVIGPDYEYGHRTWEDFWMLLGKKRTDVKMVGQAWPKLYEPDYTPY
                     ITAILNAKPDVLVTTFWGGDTVAFIKQALPYKLFDKMKMFN"
BASE COUNT          699 a          949 c         1094 g          539 t
ORIGIN      
        1 ggctccccgc aggcgcagcg gatcgtgatg ctgcgacagg cggacctgcc ggagaagacc
       61 cggcaggcga agaaggtgat cgggatccgg gacggcggcg gcgaatcgaa ggggcgttga
      121 tgagggaatt ccgcccggac ctcattctcg aggcgaccaa cgtcggaatc ctgtgcgccg
      181 atcgccaggg tcggatcctc tatgccaacg acgccgcggc ggtccagctc ggggccagga
      241 agcaggagct gatcggacgc gcgatcgacg cggtgtccaa gggggccggc gacgctttcc
      301 tcgagatcgt gcggaccgga aaaccgcagg ccggcgtgaa gatccgaacc tccggcggct
      361 tcgtgatcgc ggaccgaaac cccgtattcg acggggggaa agtgatcggc gtcgtcagcg
      421 tattcaagga catctcgcgc tacgaggaaa cggcgacaga gctccaagcg tatcgcgagc
      481 tggccaagca gcttgacgcg gtcatccact cctcctacga cggactctac atcaccaacg
      541 gcaacgccga caccctcttc tgcaacaagg cgtacctccg ggtttccggc ctcacggcga
      601 aagacgtcga ggggaagaac atgagggaaa tcgtgcgcca tgggacgatc aaccagtcgg
      661 tgaccctgga ggttctcgag aaacgccgcc gcgtcacgat catgcaggag ttctccaacg
      721 ggcggaccgc gattgtcacc ggaaacccgg tcttcgacga cgacgggaag atcatcctcg
      781 tcgtgagcaa cgtgcgggac atcacggagc ttaacgaact ccgggaacag gtccaggaga
      841 accggacgct ggcgaagcgg taccgggaag agctgaagaa ggcgagcctg gcgggtgtca
      901 accgcgacac ggtggtgttc cgctccccgg ccatggagaa ctgtatcctc ctcgcggccc
      961 gtgcgagcga tgtgctctcc ccggtgcttc tcacaggaga atccggcgtc ggaaaaggga
     1021 tgctcgcccg attgatccac aacctcggaa gccggaaaaa gggtccgttc atccacgtca
     1081 actgcggagc gatccccgcg gggctgatgg agtcggagtt cttcggctac gaaaaggggg
     1141 ctttcaccgg cgcgtcggag gaggggaagc ccgggctgtt cgaaatggcg gatcggggga
     1201 cccttttcct cgatgaaatc ggggagatcc cccttccgct gcaggtgaag cttctgaagg
     1261 ttctcgagga gagcgaggtc cgccgggtgg ggggaaccca gccgcggaaa ctggacgtgc
     1321 ggatcgtcgc ggcgacgaac cgtgacctgc aggaaatgac ccggaaccgc ctcttccggg
     1381 aggacctgtt cttccggctc gacgtgtttc ccatccgcat ccctcccctg cgcgaacgcc
     1441 cggaagatat ccccccgctg atcgatcgca ccctcggtga attgaaccga agatacaaga
     1501 aggggaaacg ggtgcgaccc gatacgctcg acctgctcgt gcgccaccct ttccctggaa
     1561 acgtccgcga acttcaaaac gtcgtggagc gttcctttat tctcgcggac gggaagtgga
     1621 tcgtgccgga gcacctcccc acggggatcc ggaaaagttc caccccgccg gaatcgggca
     1681 cggtagcgca catctcgcgg gggaaggagg gattggagca gatcctcgcc ggggtggagc
     1741 gggaaatcct cgaaaggctt ctcttggaat gcgggacgac ctacgcgatg gcggaccgat
     1801 tgaaggtcaa ccagtccacc gtcgtacgga aactgcgaaa gcttggaatc cgcgctcatc
     1861 cggcaggatg agcgtccctt gatgcgtcac tgcatcgaac tttcctccgg gcgcttcgcc
     1921 aacgtccagc cggtttcctg aataactctg ttatatcaaa cgatttcatt cggaaccgca
     1981 tcccgggact tatttggaac gatgtttgct gttttccttc catccgtggg aggaaacgtg
     2041 ggagaagccg acgtcaaggg cgctcgaacg gcaggtaggc aggatgacgg cggcgaaccg
     2101 gatgccgttt tctcccgcgg cagcggcgtg gcgtatcggt acgcgctgcg ccttcgggat
     2161 ccggaggagg cgggcacgat cctgcagatc cttgcggatg ttgcgatccc cctgctgtac
     2221 ctgctgctgc ttccccggga tcaccggagc gggtacaggg cgtacatcgg gcttgcttct
     2281 cccgatgtgc tcgatctgcc gatccggttc gcgtcccgcg gcatcatgat cgagcgcggg
     2341 gaggaaacgg gcgacagacg ccgttaatgc gcttacgcat caaacaatgc aataatgcat
     2401 cgccgggacc acgcgttaaa gagagggtcg atgaaggaga agaggatcgg cctgggcaag
     2461 gggaagtgag acagcggaac aaacacggaa gaaggaggga aacgatgcgg aagcaaggaa
     2521 gaaagtggca ggcaatcctg gtgatcggcc tggtcctcgt catgggactg gccgacgtag
     2581 cgctggcggc ggaaaagatc gtcatcggcg ggatgtaccc gatgacgggc cgcggcggac
     2641 ggtacgggct ggactcggtg gcggcggcgg agatcgcggc ggaggagatc aacgcgaagg
     2701 gcggcgtgaa cggccggatg atcgacctca tcttcaccga cgacaagtcc aacacgtcgt
     2761 acggggtgaa ggtggccaac cggtacatca aggaggacaa ggtcaacttc ctgatgggcg
     2821 tggtgagcag cgcggtgggg ctcgcggtca ccgaggtgtc gaaggagaac aagatcatct
     2881 tcgtcgggac cgaccacgcc tccacggcgc tgacgatgga gaagttccag ccgtactact
     2941 tccgcgtgtc gaacaacacg taccagtccg ccgcggccgc cgcgctgtac gcgacggaga
     3001 agaaggagtg gaagaagtat tacgtgatcg gaccggacta cgagtacggg caccggacct
     3061 gggaagattt ctggatgctg ctgggcaaga agcgcacgga cgtgaagatg gtcgggcagg
     3121 cgtggccgaa gctctatgag ccggactaca ccccctacat cactgcgatc ctgaacgcca
     3181 agcccgacgt cctggtcacc accttctggg gcggcgacac cgtggcgttc atcaagcagg
     3241 cgcttccgta caagctgttc gacaagatga agatgttcaa c
//
LOCUS       DASVJL010000035         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1787891, whole genome shotgun sequence.
ACCESSION   DASVJL010000035 DASVJL010000000
VERSION     DASVJL010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787891"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /gene="dapB"
                     /locus_tag="VF357_00660"
     CDS             complement(<1..467)
                     /gene="dapB"
                     /locus_tag="VF357_00660"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX7522684.1"
                     /translation="MPGVVVCGAMGRMGRAILGILKEGPNGFSLSGAVEAEGHPLLSQ
                     DAFEAAGAGRAGVPVTSDFAKAVAAADVAIDFTGPDSSASNAGTAAAAGKPIVIGSTG
                     LGPTHMERIRNAAAKVAIVQSPNMSVGVNLMFKVAADVARVLGEEYDVEIVET"
     gene            complement(478..1350)
                     /gene="dapA"
                     /locus_tag="VF357_00665"
     CDS             complement(478..1350)
                     /gene="dapA"
                     /locus_tag="VF357_00665"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX7522685.1"
                     /translation="MFHGAIVATITPFRNGKLDESALKKLVEFQIRNGTDGIVPCGTT
                     GESATLAYEEHERVIDLVLEAAAGRVPVIAGTGSNNTKEAVLLTRYAKKAGANAALVI
                     TPYYNKPTQAGLIAHFREVAASADIPLILYNVPSRTGVNMTAETVVRLSEVKNIVGVK
                     EASGNLTQVCDILKMTPRTFCVLSGDDGLYFPMLALGAKGVISVVSNVAPREMADLYD
                     AFALGDVARAREIHFRLWPLMKALFIETNPIPAKTALAMMGKIREEFRLPLCGMSDGS
                     RKVLAKVLSDMKIA"
     gene            complement(1368..2198)
                     /gene="dapF"
                     /locus_tag="VF357_00670"
     CDS             complement(1368..2198)
                     /gene="dapF"
                     /locus_tag="VF357_00670"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX7522686.1"
                     /translation="MTRKGIPFSKLNGSGNDFLLIDNHGDAMRGVDRTSFVAKVCDRS
                     RSIGADGVIVIEPSRRADFRWDFYNADGSRAEMCGNGGRCAARFAATRRIAGREMSFE
                     TIAGILRASVRGRRVKLQMTRPRGLAVNRSLTLAGKKFSYSFLDTGVPHAVLFVPDVS
                     RADVEGVGRGIRRHKAFAPRGTNVNFVQSVGDGLLVRTYERGVEGETLACGTGAVASA
                     ILAAVRGLATPPVTVRTSGGETLVIHFDPKRTDFGEVFLEGDTSWSCDGKIFEEAYRY
                     "
     gene            complement(2265..3560)
                     /gene="lysA"
                     /locus_tag="VF357_00675"
     CDS             complement(2265..3560)
                     /gene="lysA"
                     /locus_tag="VF357_00675"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX7522687.1"
                     /translation="MHHFQIRNGEMHCEGVPLRRIARDVGTPVYVYSHATLAHHYRVF
                     DEAFGGIPHIICFSMKSNSNGSVIRTFTGLGSGVDIVSGGELVRALAAGAPPGKIVYS
                     GVGKTVAEIEEALRRGILMFNVESREELETIDAVAGRMRKRAPIAIRVNPDVDPKTHP
                     YISTGLKKNKFGIRIAQAMKDYEWAAGRRHIEIVGVDCHIGSQLTDVAPFVDAAGRVR
                     RLVDRLLRMGLPIRFVDIGGGLGIRYDDELPPDPVTYAAAVAGAFRGLPVTLVLEPGR
                     VLVGNAGVLLTEVLYTKPIPSPAGRGKKHFFIVDAAMNDLARPSLYGSYHAILPVGKA
                     RRGKVTADVVGPICESGDFLAKDRAMPPCRGGDLLAVMSAGAYGFSMSSNYNTRPRAA
                     EVMVSGSRFEVVRLRETVRELYRGERTASFLSRRRTGKG"
     gene            complement(3565..>3767)
                     /locus_tag="VF357_00680"
     CDS             complement(3565..>3767)
                     /locus_tag="VF357_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522688.1"
                     /translation="DARRDGRRGGPGSPVVAPQEVMALARAALALAVLLTMLGSAGCG
                     RKGMPEPRKSESSSTATLIGAR"
BASE COUNT          610 a         1369 c         1163 g          625 t
ORIGIN      
        1 tgcgtctcga cgatctccac gtcgtactcc tcccccagca cccgcgccac gtcggccgcg
       61 accttgaaca tgaggttcac gcccacgctc atgttggggg attggacgat cgccaccttc
      121 gccgccgcgt tccggatccg ctccatgtgg gtcggaccga gccccgtgct cccgatgacg
      181 atcggttttc cggccgcggc cgccgtgccg gcgttgcttg ccgaagagtc gggaccagtg
      241 aagtcgatcg ccacgtccgc ggcggcgacc gccttcgcga agtcactggt gacgggaacc
      301 cccgcccttc cggctccggc cgcctcgaag gcgtcctgcg aaagaagggg atgtccctcc
      361 gcctcgaccg ccccggagag ggagaatccg tttggaccct ccttcaggat gccgaggatc
      421 gccctcccca tccgccccat cgcgccgcag acgacaactc cgggcaaggc cgtcccccta
      481 cgcgatcttc atgtcggaga ggaccttcgc gaggactttc cggctgccat cggacatccc
      541 gcacagcggg agccggaact cttcgcggat cttccccatc atggcaagcg ccgtcttggc
      601 ggggatcgga ttcgtctcga tgaaaagcgc cttcatcagg ggccacaggc gaaagtggat
      661 ctcccgcgct cgcgccacgt cccccagagc gaaggcgtcg tagaggtcgg ccatctccct
      721 cggggcaacg ttggagacga cggaaatgac ccccttggcg ccgagggcga gcatcgggaa
      781 gtagagcccg tcgtctccgg acagcacgca gaaggtcctc ggggtcatct tcaggatgtc
      841 gcagacctgc gtgaggttcc cggaggcctc cttcaccccg acgatgtttt tcacctcgga
      901 gaggcgcacg acggtctccg ccgtcatgtt gaccccggtg cgggacggga cgttgtagag
      961 gatgagcggg atgtccgcgg aggcggccac ctcccggaag tgcgcgatca accccgcctg
     1021 ggtcggcttg ttgtaatacg gggtgatgac gagcgccgcg ttcgcccccg ccttcttcgc
     1081 gtagcgggtc agcaggacgg cttccttcgt attgttcgac cccgttcccg cgatgaccgg
     1141 cacccgcccg gccgccgctt cgagcaccag gtcgatcacc cgctcgtgct cctcgtaggc
     1201 gagcgtagcg gactccccgg tcgtgccgca cggaacgatc ccgtcggtcc cgttccggat
     1261 ctggaactcc accagttttt tcagcgcgga ctcatccagt ttaccgttcc ggaacggcgt
     1321 gatggttgcg acgatggccc cgtggaacat tcggtcgtcc ccccttttca gtagcgatac
     1381 gcctcttcga agatctttcc gtcacacgac caggaggtgt caccctcgag gaagacctcc
     1441 ccgaaatcgg tccgcttcgg gtcgaaatgg atgacgagcg tctcgccgcc gcttgtccgc
     1501 accgtcaccg gcggcgtcgc aagaccgcgg accgccgcca ggatcgcgct ggcgaccgca
     1561 cccgttccgc acgcaagcgt ttccccctcg accccccgct cgtaggtgcg caccagcagg
     1621 ccgtcgccga cggactgcac gaagttgacg ttggtccccc ggggggcgaa cgccttgtgc
     1681 cgccggatcc cgcgcccgac cccctccacg tccgcccgag agacgtccgg gacgaacagg
     1741 acggcgtggg gaacgccggt gtcgagaaac gagtaggaga acttcttccc cgccagcgtc
     1801 agcgaccggt tgaccgccaa cccgcggggc cgcgtcatct ggagcttcac gcgccgcccc
     1861 cgcacggaag cgcggaggat ccccgcgatc gtctcgaagg acatctcccg gcccgcgatc
     1921 cggcgggtcg cggcgaagcg cgccgcgcac cggcccccgt tgccgcacat ctcggcgcgg
     1981 gaaccgtcgg cgttgtagaa gtcccaccgg aagtccgccc gccgcgacgg ctcgatcacg
     2041 atcaccccgt cggcgccgat cgagcgggac cggtcgcaca ccttcgcgac gaaggaagtg
     2101 cggtcgactc ctcgcatcgc gtcaccatgg ttgtcgatca ggagaaagtc gttgccgctc
     2161 ccgttcagct tggaaaaagg gattcccttc cgcgtcatcg tcccgccctc tccgctctcc
     2221 atcttcgaat atcgtaacag aaccgcggga gatcgaggaa gagatcatcc ttttccggtg
     2281 cgccggcggg agaggaacga cgcggtccgc tcgccgcggt acagttcccg taccgtctcc
     2341 cgcaaccgga cgacttcgaa ccggctcccg gataccatca cctcggcggc gcgcggccgc
     2401 gtgttgtagt tcgaggacat ggagaagcca tacgcccccg cgctcatcac cgcgaggaga
     2461 tccccccccc ggcacggcgg catcgcgcgg tccttcgcca gaaaatcccc tgattcgcag
     2521 atagggccga cgacgtccgc cgtcactttt ccgcggcgcg ctttccccac cgggaggatc
     2581 gcgtggtacg agccgtagag cgaggggcgc gccagatcgt tcatggcggc gtcgacgatg
     2641 aagaagtgtt tcttccccct cccggccggg gacgggatcg gcttcgtgta gagcacctcg
     2701 gtgagcagaa cccccgcgtt gccgacgagg acccgacccg gctcgagcac gagcgtgacc
     2761 ggaagacccc ggaacgcccc cgcgaccgcg gccgcgtacg tcaccggatc cggggggagt
     2821 tcgtcgtcgt accggatccc cagccccccc ccgatgtcga cgaagcggat gggcaacccc
     2881 attcggagca ggcggtcgac gaggcggcgg acccgccccg cggcatcgac gaagggggcg
     2941 acgtcggtca actgcgaacc gatgtggcag tccaccccca cgatctcgat gtgacgccgc
     3001 ccggcggccc actcgtaatc cttcatcgct tgcgcgatcc ggatcccgaa cttgttcttc
     3061 ttcagccccg tcgaaatgta cgggtgggtc ttcggatcca cgtcggggtt cacgcggatc
     3121 gcgatgggcg cccgcttccg catccttccg gccactgcgt cgatcgtctc cagctcctcg
     3181 cgcgactcca cgttgaacat gaggatcccg cggcgcagcg cctcctcgat ctccgcgacc
     3241 gtcttcccga cccccgagta gacgatcttc cccggaggcg ccccggcggc gagcgcccgc
     3301 acgagctcgc cccccgagac gatgtcgact ccgctcccca ggccggtgaa ggtccggatg
     3361 acggagccgt tcgagttcga cttcatcgaa aaacagatga tgtgcgggat cccgccgaac
     3421 gcctcgtcga agacacggta gtggtgggca agggtcgcgt ggctgtacac gtacaccggc
     3481 gtccccacgt cccttgcgat tcgccgcagc gggaccccct cgcaatgcat ctctccgttc
     3541 ctgatctgga aatgatgcat cgactcacct cgcaccgatc agagttgccg tcgacgagct
     3601 ttcgcttttc cgaggctcgg gcatcccctt gcgcccgcac cccgcggatc cgagcatcgt
     3661 caacaggacg gccagcgcga gcgctgcgcg cgcgagcgcc atcacttctt gcggagcgac
     3721 gacaggcgat cccggaccgc ctctgcgccc gtcccgccgc gcgtctt
//
LOCUS       DASVJL010000036         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_662598, whole genome shotgun sequence.
ACCESSION   DASVJL010000036 DASVJL010000000
VERSION     DASVJL010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662598"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..717)
                     /locus_tag="VF357_00685"
     CDS             complement(97..717)
                     /locus_tag="VF357_00685"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7522689.1"
                     /translation="MNIRAWILLAAMFALFPSTSPEAASADAPASATIRGACDIAFVA
                     TSTLHDVSGSARCLPFAAVLARDAAGRQVIPSVEVEVPVAGMDTRDKSRDGQMREMFR
                     SERFPRIHAAAQDVDVERLRVEIGKGREGNASIDLLLRIRDVERKVRATASNLKESGE
                     RVTFDLEFPVSLGEFDLKAPSVLGIIRVGDKVSVKATFTLIVSRSP"
     gene            complement(989..>1884)
                     /locus_tag="VF357_00690"
     CDS             complement(989..>1884)
                     /locus_tag="VF357_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7522690.1"
                     /translation="FKPVGLVETARGCPHRCSFCSVWQLYGRSFRERSIGAVVEDFAA
                     AGDSIFVADDLFWNHAERSLELADALRKRGVKKRWILVQTRTDMICRQQGLLEAWRPL
                     AKDFDIFLGLEAATDAGLSRVDKDVPVSESVEAVRIARSLRYGVNGNFLVDPDWGEED
                     FRRLWEFVACHGLQRAGYTVLTPLPGTDYFRKIAPRLAGQPWSNYDMHHVLWEPRLGA
                     RRFFELYAETWRKSILNTSGEKRWTEWMRQIRPAQIPYLARVLWRTQRMMKAGAYLRE
                     HGKTSRAPMPVRSPLGNGASR"
BASE COUNT          345 a          608 c          595 g          336 t
ORIGIN      
        1 cctcctcctg ggcgaaggag aaatcgggga gcagcgtgtc gatccgttgg atccaggcgg
       61 tcattggaac ctccgtctat taaagtagga tgcgtctcaa ggggatcggg aaacgatcag
      121 ggtgaaggtg gccttgacgg agaccttgtc gccgacccgg atgatcccga ggacggaggg
      181 ggctttcaga tcgaactccc cgagcgacac ggggaattcg aggtcgaagg tgacccgttc
      241 gccggactcc ttcaggttgc tcgccgtcgc acggaccttc cgctccacat cccggatccg
      301 gaggagcagg tcgatggagg cattcccctc gcgacccttt ccaatttcca cccggagccg
      361 ttcgacgtct acatcctgcg ctgccgcgtg gattcgcgga aaccgctccg accggaacat
      421 ctcccgcatc tgcccgtcgc gggacttgtc ccgcgtgtcc atccctgcca ccggcacttc
      481 cacttccacg gatgggatga cctgcctgcc cgcggcgtcg cgagcaagga ccgctgcaaa
      541 gggcaggcaa cgggcggagc cggaaacatc gtgcagggtg gacgtggcga cgaatgcgat
      601 gtcgcacgct ccccggatcg tcgccgaagc gggggcgtcc gccgaagcag cctccggtga
      661 ggttgagggg aaaagggcga acatcgccgc cagcagtatc cacgcacgga tattcatccc
      721 gggcctcccc ttctccactt tcattatatt gatgcctcgc ctcatcaatg tgaatacggt
      781 aacggtggcg tccttccctt tgcgtgatcc gccgtccgat gcgccttttc attggatgcg
      841 gaaatcatga aagggagtcc tggaatgacg atctccctcg ccaacctcac cgccctgcga
      901 cagaggatcc ggatcgagga agaggcgttg gcggaaatcg tcgcgtcggg gggggcgagg
      961 tcccgagcta gtgcaatgtc acggtacact agcgcgatgc gccgttgccc aagggggacc
     1021 ggacgggcat cggggcacga ctcgtcttgc cgtgctcgcg caggtaggcc ccggctttca
     1081 tcatcctctg ggtgcgccaa agaacgcgcg caaggtacgg tatctgggcc ggccggatct
     1141 gccgcatcca ctcggtccac cgtttctcgc ccgacgtgtt gaggatcgac ttgcgccaag
     1201 tctccgcgta gagttcgaaa aaccgtctcg ccccgaggcg aggctcccag aggacgtggt
     1261 gcatgtcgta gttggaccag ggctgtcccg cgagtcgggg cgcgatcttg cggaaataat
     1321 cggttcccgg caacggagtg aggacggtgt accccgcccg ctgcaacccg tggcaggcga
     1381 cgaactccca gagccgacgg aaatcctcct ctccccagtc gggatcgacc aggaagttgc
     1441 cgttcacgcc gtatcggagg gaccgcgcga tcctcaccgc ctcgacgctc tccgaaaccg
     1501 gaacgtcctt gtcaacccgg gacaggcccg cgtcggtcgc ggcctccagt ccgaggaaga
     1561 tgtcgaagtc cttcgcgagg ggcctccacg cctccagcag tccttgctgc cggcagatca
     1621 tgtcggtccg ggtttgcacg aggatccagc gttttttcac tccgcgcttc cggagggcgt
     1681 ccgcgagttc gaggctccgt tcggcatggt tccagaagag gtcgtcggcg acgaagatgg
     1741 aatcccccgc cgcggcgaaa tcttcgacga cggccccgat ggaccgttcc cggaaggatc
     1801 gcccgtacag ctgccacacg gaacagaaac tgcaccggtg cggacatccg cgggccgtct
     1861 ccacgagtcc caccggcttg aaga
//
LOCUS       DASVJL010000037         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1225627, whole genome shotgun sequence.
ACCESSION   DASVJL010000037 DASVJL010000000
VERSION     DASVJL010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225627"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VF357_00695"
     CDS             <1..894
                     /locus_tag="VF357_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX7522691.1"
                     /translation="LPVDSLLIGSTGVIGVPLPVKKIVAALPGLAARLSPKGIAGAGE
                     AIRTTDAYPKRGIRKIREGGRTITIGGIAKGAGMIAPNMGTLLAYAFTDAALAPADAR
                     RLLREAADATFNRIVVDGDTSTNDTAALFANGACGLPPLEGKGLAAFREALLSLLLDL
                     ALMVVRDGEGATRVVRLSVTGAVTGADATKAARAVASSPLVKTAVYGADLNWGRVIAV
                     LGRAGIALDPMRISMRFAGETLLRRGMRPDPAAERRAAPKIRTEAYAIDVDLGLGKGC
                     DYAYFSDLSVEYVKLNSGYRS"
     gene            1031..1873
                     /gene="rpsB"
                     /locus_tag="VF357_00700"
     CDS             1031..1873
                     /gene="rpsB"
                     /locus_tag="VF357_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX7522692.1"
                     /translation="MANGQSAVISMKQLLEAGVHFGHQTKRWNPKMKRYIFTARNGIY
                     IIDLQQTVKMFRAAYEAVRSMAAEGKTVLFVGTKKQAQEAVEEEARRAGTPYVNQRWL
                     GGMLTNFTTIRKSLDRLQKLSEIGTDGTAERLPKKEVLQLEKERVKLEKTLGGIRDLK
                     RVPDAMFVVDPSRETIAILEGRRLGIPIVAIVDTNCDPDLIDVVIPGNDDAIRAIKLF
                     LSKMADAILEGKAAYAEKNASRVDKEAEAPEVTVSLISTEGDEEIPIATKPSVATQTP
                     AAAE"
BASE COUNT          368 a          631 c          729 g          278 t
ORIGIN      
        1 ctgccggtcg attcgctgct gatcggctcc accggcgtca tcggggtgcc gctgccggtg
       61 aaaaagatcg tcgcggccct gccgggcctc gccgcccgtc tctccccgaa ggggatcgcc
      121 ggggcggggg aggcgatccg cacgacggat gcctacccga agcgcgggat ccggaagatc
      181 cgcgaggggg ggcgcacgat cacgatcggc gggatcgcga agggcgccgg gatgatcgcc
      241 ccgaacatgg gaacgttgct cgcctacgcg ttcaccgacg cggccctggc tcccgccgac
      301 gcccggcgtc ttctgcggga ggcggccgac gcgacgttca accgcatcgt cgtcgacggc
      361 gacacgagca ccaacgacac cgcggccctc ttcgccaacg gcgcgtgcgg tttgcccccg
      421 ctggaaggga agggcctcgc cgcgttccgg gaggcgctcc tgtcgctgct gctggatctc
      481 gccctgatgg tcgtgcgcga cggcgagggg gcgacccggg tggtccgtct gtcggtcacc
      541 ggcgcggtca ccggcgcgga cgcgacgaag gcggcccgtg cggtcgcttc ctccccgctg
      601 gtgaaaaccg ccgtctacgg cgcagacctg aactggggcc gggtgatcgc ggtgctcggg
      661 agggcgggga tcgcgttgga cccgatgagg atctcgatgc ggttcgccgg ggaaacgctg
      721 ctgcgccggg ggatgcgccc ggacccggcc gcggagcgcc gcgccgcccc gaagatccgg
      781 acggaggcgt acgccatcga cgtcgacctc gggttgggga agggatgcga ctacgcctac
      841 ttctccgatc tgagcgtcga gtatgtgaag ttgaactcgg ggtatagatc gtgatataag
      901 tgcaaggctt agctgcagat tatcacccac gggcgggact ctccccgggg tgccccgtat
      961 cgacggggta aatggggagg ggcggagcgc ccggcggaac aacccctgaa ccgaaggagg
     1021 aaagaaaggg atggcgaacg gacagagcgc ggtgatctcg atgaagcaac tgctcgaggc
     1081 gggggtccac ttcgggcacc agacgaagcg ttggaacccg aagatgaagc ggtacatctt
     1141 cacggcacgc aacgggatct acatcatcga tcttcagcag acggtgaaga tgttccgggc
     1201 cgcctacgag gcggtgcgga gcatggcggc cgaggggaag acggtcctct tcgtcggcac
     1261 gaagaagcag gcgcaggagg cggtggagga ggaggcccgt cgggccggca cgccgtacgt
     1321 caaccagcgc tggctgggcg ggatgctcac caacttcacc acgatccgga agagcctcga
     1381 ccggttgcag aagctttcgg agatcggaac cgacggcacc gccgagcggc tcccgaaaaa
     1441 ggaagtcctt cagctcgaga aggagcgggt caagctcgag aagaccctcg gcgggatccg
     1501 ggatctcaag cgcgtgccgg acgcgatgtt cgtcgtcgac ccgtcgcggg agacgatcgc
     1561 gatcctcgag ggccggcggc tggggatccc catcgtcgcc atcgtggaca ccaactgcga
     1621 ccccgacctg atcgacgtcg tgatccccgg gaacgacgac gccatccgcg cgatcaagct
     1681 gttcctctcc aagatggccg acgcgatcct cgaggggaag gcggcctacg cggagaagaa
     1741 cgcctcccgc gtcgacaagg aggcggaggc gcccgaggtg acggtgtcgc tcatctccac
     1801 cgagggggac gaggaaatcc ccatcgcgac gaagccttcc gtggcgacgc agacccccgc
     1861 cgcggcggaa taattccggg tcccgagcgg cccgaacgat cagggagatc cagggaaatg
     1921 cagatcacat ccgggatggt caaggagctc cgcgagaaga ccggcgcggg gctgatggat
     1981 tgcaaggcgg ccctcacggc gtcgga
//
LOCUS       DASVJL010000038         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_287527, whole genome shotgun sequence.
ACCESSION   DASVJL010000038 DASVJL010000000
VERSION     DASVJL010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287527"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VF357_00705"
     CDS             <1..503
                     /locus_tag="VF357_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX7522693.1"
                     /translation="HAAAKALRETPIMNATVSGESTVLKKDIHIAVAVDTEKGLLVPV
                     LRHADRMSLKEISAAMDDFGARAKSGKISPDELSGGTFSITNPGIKGNLFGTPIINQP
                     QVGVLRLGQIVKRPVVIDADGDDSIVIRPMMYLCCAYDHRVIDGVAGNGFLYRVREIL
                     ESGDFS"
     gene            520..1146
                     /gene="lipB"
                     /locus_tag="VF357_00710"
     CDS             520..1146
                     /gene="lipB"
                     /locus_tag="VF357_00710"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX7522694.1"
                     /translation="MEIVWLGEVGYREALELQLSCLDRRAARRIPDTLLLLTHPHVYT
                     LGRAGDEANLLVPRESLAAEGVPVERVGRGGDITYHGPGQLVGYPIVLMEKPDVHKFV
                     RSIEAALIDAVGAFGVESRRIEGLTGVWAGERKIASIGVGIRKWVTYHGFALNVTTDL
                     SWFRRIHLCGLKGREATSIAEETGNAPTMETVREAVAAACDRHLEGFA"
     gene            1143..>1906
                     /gene="lipA"
                     /locus_tag="VF357_00715"
     CDS             1143..>1906
                     /gene="lipA"
                     /locus_tag="VF357_00715"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX7522695.1"
                     /translation="MIPNRAPRPDWLKVRAPSGKRMEEVSETLARLGLRTVCREARCP
                     NVGECWGAGAATVILLGDVCTRGCAFCNVTAGVPAPPDPAEPELVAKAAAELSWRHVV
                     VTSVTRDDLPDGGAAHFASVVRALRREASEATVELLVPDFAGNVDALRCVVDAAPDIL
                     AHNVETVPRLYPVVRKGAEYGRSLELLRRAAAMRPSLLLKSGIMVGFGETEVEVVAVF
                     RDLFAAGCRSITVGQYLPPSRDHLPLSEYVSPERFDL"
BASE COUNT          301 a          611 c          682 g          312 t
ORIGIN      
        1 tccacgccgc ggcgaaggcg ctgcgtgaga ccccgatcat gaacgccacc gtgtccggag
       61 agagcacggt gttgaaaaag gacattcaca tcgccgtcgc ggtcgacacg gagaaaggcc
      121 tgctcgtccc ggtgctgcgc cacgcggacc ggatgtccct gaaggagatc tcagcggcga
      181 tggacgactt cggcgcgcgg gccaagtcgg ggaagatctc cccggacgaa ctctcggggg
      241 gaaccttcag catcaccaac ccggggatca aggggaacct cttcggcacc ccgatcatca
      301 accagcccca ggtgggggtc ctccggctgg ggcagatcgt gaagcggccc gtggtgatcg
      361 acgcggacgg cgatgactcc atcgtaatcc ggccgatgat gtacctctgc tgcgcatacg
      421 accaccgcgt catcgacggc gtggcgggaa acggctttct gtaccgtgtc cgggagatcc
      481 tcgaatcggg ggatttctcg tagggcgaaa gggcgcaccg tggagatcgt ctggctgggg
      541 gaggttgggt accgggaagc gctcgagctc cagctctcct gcctggaccg cagggcggcc
      601 cgacggatcc cggacacctt gcttcttctc acccacccgc acgtttacac cctgggccgc
      661 gccggagacg aggcgaacct gctcgtcccg agggaatcgc tcgcggcgga gggggtcccg
      721 gtggagcgcg tcgggcgggg cggcgacatc acctatcacg ggccggggca actggtcggc
      781 tacccgatcg tcctgatgga gaagccggac gtccacaagt tcgtccggtc gatcgaggcg
      841 gcgttgatcg acgccgtggg cgcgttcggc gtcgagtccc ggcggatcga ggggctgacg
      901 ggtgtctggg cgggggagcg gaagatcgcc tccatcggcg tcggcatcag gaagtgggtc
      961 acctaccatg gcttcgccct gaacgtgacg accgatcttt cctggttccg gcggatccac
     1021 ctgtgcggcc tgaaggggcg ggaggcgacg tcgatcgccg aggaaacggg gaacgctccg
     1081 acaatggaga cggtacgcga ggcggtggcc gccgcctgcg accgtcatct ggaggggttc
     1141 gcatgatccc gaaccgcgca ccgcgtcccg actggctcaa ggtccgcgct ccttccggaa
     1201 aacggatgga ggaggtgtcg gagacactcg ccaggctcgg tctgcggacg gtgtgccggg
     1261 aggccaggtg ccccaacgtg ggggagtgtt ggggggcagg ggcggcgacc gtgatactgc
     1321 tcggcgacgt gtgcacgcgg ggttgcgcct tctgcaacgt gaccgccggg gtgcccgctc
     1381 cgcccgatcc cgccgaaccg gagcttgtgg cgaaggcggc ggcggagctc tcctggcggc
     1441 acgtggtcgt cacctccgtc acccgcgacg atctgcccga cggcggggcg gcgcacttcg
     1501 cctccgtggt ccgcgcgctg cggcgggaag cgtcggaggc cacggtcgaa cttctggttc
     1561 cggacttcgc cggaaacgtc gacgcgctgc gatgcgtcgt cgacgccgcc cccgacatcc
     1621 tcgcccacaa tgtggagacc gtcccgcgcc tctatccggt ggttcggaaa ggcgcggagt
     1681 atggccgatc cctcgaactt ctccgccgcg ccgccgcgat gcgtccctcc ctgttgctga
     1741 aatccgggat catggtcggg ttcggggaga ccgaagtgga agtggtcgcg gtcttccggg
     1801 acctgttcgc cgcggggtgc cgctcgatca cggtggggca atacctgccg ccttcaaggg
     1861 atcatcttcc gttgtccgaa tacgtctccc cggaacggtt cgacct
//
LOCUS       DASVJL010000039         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_287545, whole genome shotgun sequence.
ACCESSION   DASVJL010000039 DASVJL010000000
VERSION     DASVJL010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287545"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="VF357_00720"
     CDS             <1..1516
                     /locus_tag="VF357_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7522696.1"
                     /translation="IKEEQLTEALRSQSQLGGTLGENLIRLAFLTEEELLTALSEQLG
                     MQHINLAKVEVPAAVQRLVKMETVRLRRLLPIGFEGKRLVVGMVDPTDISALTEVEFQ
                     SGHTTKPVILSASHFELAQAFFQAHGYGDATLKFDAEKEAARHVRVENTLASMLTVLL
                     SWKGQDLHLSAGAIPSIRVDNEIRRLNLPVLKPAEIEQMIYAILTQEQRRYFQENLEL
                     DFAFSLHGVGRFRCNLYRQRNSVAFTARHVSEEVPSAAELGIPDFLRDFGLKNQGLIL
                     ITGPNGHGKSTTLAWLVDMINRDRRSNIITIEDPVEFTHRHKNSNVNQREVGTDTLSF
                     ADGLRHIFRQNPDVIVIGELRDYESISIALTAAETGHLVLGTLHSMNATAAVDRIVDS
                     FPANQQSQVRAQLAESLLLVFSQRLLKRANGSGRVLAWEKMATSLRVRNAIREGKVHQ
                     LRGMMQTNVDEMVSIDWTLADMVAAGKVKYEEAVKFADNLTYLNELLKVRGAFK"
BASE COUNT          319 a          571 c          555 g          264 t
ORIGIN      
        1 catcaaggag gagcagctga ccgaggcgct gcggagccag agccagctcg gggggacgct
       61 gggggagaac ctcatccggc tggcgttcct cacggaggag gagctgctga cggccctctc
      121 cgagcagctg gggatgcagc atatcaacct cgccaaggtc gaagtccccg ccgcggtgca
      181 gcggctggtc aagatggaaa ccgtccgcct gcgccgcctc ctccccatcg ggttcgaggg
      241 gaagcgcctg gtggtcggca tggtcgaccc cacggacata tccgccttga ccgaagtgga
      301 gttccagtcg gggcacacca cgaagccggt catcctgtcc gcttcgcact tcgagctggc
      361 ccaggcattc ttccaggcgc acgggtacgg ggatgccacg ctgaagttcg atgcggagaa
      421 ggaggcggca cggcacgtcc gggtggagaa caccctcgcc tcgatgctca cggtgctcct
      481 ctcgtggaaa gggcaggacc tgcacctgtc cgcgggggcg atcccgtcga tccgcgtcga
      541 caacgagatc cgccggctga acctcccggt cctcaagccc gcggagatcg agcagatgat
      601 ctacgcgatc ctcacgcagg agcagcgccg ctacttccag gaaaatctcg agctcgactt
      661 tgcgttctcc ctccacgggg tcggccgctt ccggtgcaac ctgtaccgtc agcgcaactc
      721 ggtcgccttc accgcgcgcc acgtgtccga ggaggtcccg tcggcggcgg agctcgggat
      781 ccccgacttc ctgcgcgact tcggactgaa aaaccagggg ctgatcctca tcaccggccc
      841 gaacggccac gggaagtcga ccacccttgc gtggctcgtc gacatgatca accgggatcg
      901 gcggtccaat atcatcacga tcgaggatcc cgtcgagttc acccaccggc acaagaactc
      961 caacgtgaac cagcgggagg tggggacaga caccctctcc ttcgcggacg gccttcggca
     1021 catcttccgg cagaatccgg acgtcatcgt gatcggggag ctgcgcgact acgagagcat
     1081 ctcgatcgcc cttaccgccg ccgagacggg acatctggtc ctggggacgc tccactccat
     1141 gaacgccacg gccgccgtgg accggatcgt ggactctttc ccggcgaacc agcagtcgca
     1201 ggtccgcgcg cagctggccg agtcgctgct tctcgtcttc tcccagcgcc tgctcaagcg
     1261 cgccaacggt tcggggcgcg tgctcgcgtg ggaaaagatg gcgacctccc tccgggtccg
     1321 caacgccatc cgggagggaa aggtccacca gctgcggggg atgatgcaaa ccaacgtcga
     1381 cgagatggtg tcgatcgact ggacgctcgc ggacatggtg gcggccggga aggtgaagta
     1441 cgaggaagcg gtgaaattcg ccgacaacct gacctatctg aacgaactcc tcaaggtccg
     1501 cggagcgttc aagtagggga gccgcccgcg ggtacccccg ggcaggaggc gcatgcccga
     1561 tgatcggtaa gaagatccgc ctggagcgga tcatggaccg gaacacccac aggaccgtgc
     1621 tcgttcccat ggaccacggg gtgaccgtcg gcccgatccc cgggctgatc cagatccccc
     1681 cggcggcgaa cctcatcgcc gaggggggg
//
LOCUS       DASVJL010000040         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_194044, whole genome shotgun sequence.
ACCESSION   DASVJL010000040 DASVJL010000000
VERSION     DASVJL010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194044"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VF357_00725"
     CDS             <1..637
                     /locus_tag="VF357_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7522697.1"
                     /translation="VRGPSGGGVHGGLYHSQNPEAWFCHVPGLKVVAPATAYDAKGLL
                     KSAIRDDDPVIYFEHKFLYRRIKEEIPAEEYLVPIGKAALRKEGRDLTVITYGAPVHA
                     VMKAARDMAAEVDIEVIDLRTLLPIDWATVKASVRKTGKVLIVHEARLTGGIGGEVAA
                     RIAQDCFEELDGPVMRLAARDVHTPFAPAMEAYVLPNQEKITEAIRKLAAY"
     gene            657..>1783
                     /locus_tag="VF357_00730"
     CDS             657..>1783
                     /locus_tag="VF357_00730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX7522698.1"
                     /translation="MLIDVVMPQLGESVVEGVVVKWLVEVGVLVAKDQPLLEISTDKV
                     DAEIPSPAAGRVAQILVKPGETVPILAVLAKLETDPAASRPAPEAAPKWDEETEGVPG
                     HMARGSEPPIPAPPRVEPSAPPPPPPPTTGRIRITPVVARMAAEHGLDLSKIPGTGTD
                     GRVTRKDIEGYLASSASGSEVSAEKPKPAEVKAVSVPPTTKAAPPSSAKASEGKPPAA
                     EGDQVVAWTPIRKRIAEHMVRSKQTSPHVHIFAEVDMHRVAAVRAKEKKQGVNLTYLP
                     FVIHAAAKALRETPIMNATVSGESTVLKKDIHIAVAVDTEKGLLVPVLRHADRMSLKE
                     ISAAMDDFGARAKSGKISPDDLSGGTFSITNPGIKGNLFGTP"
BASE COUNT          329 a          578 c          634 g          242 t
ORIGIN      
        1 ggtccggggc ccgtcgggcg gcggggtcca cggcggcctg taccactccc agaacccgga
       61 ggcgtggttc tgccacgtcc ccggcctcaa ggtggtggcc ccggcgacgg cgtacgacgc
      121 gaaggggttg ctgaagtccg ccatacgtga cgacgacccg gtgatctact tcgagcacaa
      181 gttcctctat cggcggatca aggaggagat ccccgcggag gagtacctgg tccccatagg
      241 gaaggcggcg ctgcgcaagg agggacggga cctcaccgtc atcacctacg gcgccccggt
      301 ccacgcggtg atgaaggcgg cacgggacat ggccgcggag gtggacatcg aggtgatcga
      361 cctgcggacc ctcctgccga tcgactgggc gacggtgaag gcttccgtgc ggaagacggg
      421 aaaggtgctg atcgtccacg aggcgcgcct gaccggaggg atcgggggag aggtcgccgc
      481 gcggatcgcg caggattgct tcgaggaact ggacgggccg gtcatgcgcc tggcggcgcg
      541 ggatgtccac accccgttcg cccccgcgat ggaggcgtac gtcctgccga accaggagaa
      601 gatcaccgag gcgatccgga aactcgccgc gtattaaacg caaggagcgt gaagcgatgc
      661 tcatcgacgt cgtgatgccc cagctcgggg aaagcgtggt ggaaggggtc gtggtgaagt
      721 ggctcgtgga ggtgggggtg ttggtcgcca aggaccagcc cctgctcgag atctccacgg
      781 acaaggtgga cgcggagatc ccctcgccgg ccgcggggcg ggtggcgcag atccttgtca
      841 agccgggaga gacggtcccc atcctggcgg tgctggcgaa actcgagacg gacccggcgg
      901 cgtcccggcc cgcgcccgaa gcggcaccga aatgggacga ggaaaccgag ggggtccccg
      961 gccacatggc ccggggttcg gaaccgccga tcccggcgcc gccgcgcgtc gaaccgtccg
     1021 ctcccccgcc tccgccgccc ccgacgaccg gacggatccg gatcactccg gtggtggccc
     1081 ggatggcggc cgagcatggc ctggatctgt caaagatccc gggcaccggc accgacggac
     1141 gggtgacccg gaaagacatc gaggggtatc tcgcctcctc cgcctcgggc tccgaagtgt
     1201 cggcagagaa gcccaagcct gcggaggtca aggccgtctc cgttcctcca acgaccaagg
     1261 ccgcgcctcc ctcctccgcg aaagcttcgg agggcaagcc ccccgcggcc gaaggggacc
     1321 aggtagtggc gtggacgccg atccggaaga ggatcgccga gcacatggtc cggagcaagc
     1381 aaacctcgcc ccacgtccac atcttcgccg aagtggacat gcacagggtg gcggcggttc
     1441 gggccaagga gaaaaagcag ggcgtgaacc taacctacct tcccttcgtg atccacgccg
     1501 cggcgaaggc gctgcgggag accccgatca tgaacgccac cgtgtccgga gagagcacgg
     1561 tgttgaaaaa ggatatccac atcgccgtcg cggtcgatac ggagaaaggc ctgctcgtcc
     1621 cggtgctgcg gcacgcggac cggatgtccc tgaaggagat ctccgctgct atggacgact
     1681 tcggcgcgcg ggcgaagtcg gggaagatct ccccggacga tctctcgggg ggcaccttca
     1741 gcatcaccaa cccggggatc aaggggaacc tcttcggcac ccc
//
LOCUS       DASVJL010000041         5047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_100357, whole genome shotgun sequence.
ACCESSION   DASVJL010000041 DASVJL010000000
VERSION     DASVJL010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100357"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VF357_00735"
     CDS             complement(<1..617)
                     /locus_tag="VF357_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX7522699.1"
                     /translation="MSHADLAAGKVIRMSLLFRVQHLLLTVLLTVLAVTGFALMYHEN
                     SLSQWIIRLEGGVHNRGIVHRIAAILLMANLVYHVFYMFLSREGKPELRQLFIGRQDI
                     DDFLQSLRYNLGMAKEYPRFGKYGYKEKFQYWGASAGIVLISITGIMLWAEEFSMVFF
                     PKVVLDLAIVIHGYQGLLAFAVLFLWHLYNVHLHPSVFPMNPSWIT"
     gene            complement(614..1882)
                     /locus_tag="VF357_00740"
     CDS             complement(614..1882)
                     /locus_tag="VF357_00740"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX7522700.1"
                     /translation="MNRMIRHYLSAVLAATVLLSAGIAAGAATSVSECMDCHGDKTIE
                     KTQPNGQTLSLHVDLQAYRGSVHGSSGCVTCHADAKAPHEKLAKVDCGRCHGDAAKSY
                     AMSSHGKARAKGVTDVPGCHDCHGKHDIRKSKDPAARTFRLNIVETCLRCHKDQAIER
                     KYNLPGQQVMIAYQQSVHGMASRNGGLTGSAVCPDCHGNHTILPGDDPKSATFRQNIP
                     TLCGKCHSGILEKYEKSVHGKGMRAGIAESPVCTDCHGEHTISKVSSLTSPVSARNIP
                     KTCSGCHEKEGISSRYALPKKRFSSYMDSFHGIALEYGMTKAANCSSCHGVHEILPAS
                     DPASSVNKANLPKTCGKCHPNAGPNFAKGDIHVEISPKKAMGVFAVRIFYTLFIGALA
                     ILFALHVGLELYGRWNRRKEKAPGGKEGRS"
     gene            complement(1879..3927)
                     /locus_tag="VF357_00745"
     CDS             complement(1879..3927)
                     /locus_tag="VF357_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX7522701.1"
                     /translation="MMIMRRRTLRSFLTALFALTILLLLAGAASALDNGQCLDCHGDQ
                     GIPGWSPAEKASNVTPGGPKGVPHVGGNFPGMSLYVDPGLYKQSVHADVSCTDCHADI
                     KSVPHSARLKRVDCSGCHSELAAAYAKSRHVLTFSKNPIGNAPACVDCHGAHAVPKSS
                     VSTSPVYFRNIAMTCTRCHGDKTITDNRNIAIPGAAKLYQQSIHNRAIVEMGLNKSAS
                     CIDCHGAHNMKDRRDPSSSINKTNLPATCGRCHFGVYALYKESVHGTALARGVPDAPS
                     CADCHGEHDIRAVSDPNSPVSSAAISQKTCPSCHAAEKLAARYGVPVDKVRGYQQSFH
                     GLSARLGDKTVANCASCHGDHAIYPSSDPRSTVYAKNLPVTCGKCHPGATTNFASGNI
                     HVGPGGTGGRIKYLVEQLYIWMIVVVIGGMIVHNGFDYFRKMQAIYRKRRQWRQPAYE
                     RLNRSERTQHILTLSTFFTLVITGFALKFKWSIPLISDDANVALRGGSHRIAAVLMIA
                     TSLYHVFYAVFTARGRGQVWRMLPRWKDVEDIVGTIRYYIGLADHKPKFDRFSYVEKA
                     EYLALVWGTIVMVVTGFLLWFQTEAMKHIPMWGLDVATLVHFYEAILATLAIIVWHLY
                     YVFLNPDFAPMSFTWLDGKLSRHDMEHEHAIELEEIDAYVRRGETPPPDVTRIDPEEE
                     "
     gene            4490..>5047
                     /locus_tag="VF357_00750"
     CDS             4490..>5047
                     /locus_tag="VF357_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HEX7522702.1"
                     /translation="MDLNVPATFASPASAGLNDGPAHPAGKTLHDHPPQAWAEALLRK
                     HGPMPVSENARTVLERRYLKGGMKGDPLETPEEMACRVAYNIALAEGLYYGALPEVVL
                     RWAEAFYVMMLRLDFLPNSPTLMNAGRELQQLSACFVLPVEDSMESIFEAVKNTALIH
                     KSGGGTGFSFSRLRPHADVVRSTKGV"
BASE COUNT          871 a         1541 c         1711 g          924 t
ORIGIN      
        1 gtgatccacg acggattcat cgggaagacc gaggggtgca ggtggacgtt gtagagatgc
       61 cagaggaaga ggaccgcgaa ggcgaggagc ccctggtagc cgtggatgac gatcgcgagg
      121 tcgaggacga ccttcgggaa gaaaaccatg gagaactctt ccgcccacag catgatgccg
      181 gtgatcgaga tgaggacgat ccccgccgat gcgccccagt actggaactt ctccttgtac
      241 ccgtactttc cgaagcgcgg gtactccttc gccatcccga ggttgtaccg gagcgactgg
      301 aggaagtcgt cgatgtcctg cctgccgatg aagagctggc gcagctccgg cttcccttcc
      361 cgggagagga acatgtagaa gacgtggtac acgaggttcg ccatcagcag gatggcggcg
      421 atccggtgca cgatcccccg gttgtgaacg cccccctcca gccggatgat ccactgggac
      481 agggagttct cgtggtacat cagggcgaat cccgtgacgg cgaggaccgt gagcagcacc
      541 gtcaggagaa ggtgctgtac gcggaagaga agcgacatcc ggatcacttt ccctgccgcg
      601 aggtcggcat ggctcatgat cgcccttcct ttccgcccgg cgctttttct ttccttcggt
      661 tccagcggcc gtacagctcc aggccgacgt gaagagcgaa caggatcgcc agggcgccga
      721 tgaagagcgt gtagaagatc cggacggcaa acacccccat cgccttcttc ggagaaatct
      781 ccacgtggat gtcgcccttc gcgaagttgg gtcccgcgtt ggggtggcac ttcccgcacg
      841 tcttcggcag gttcgccttg ttcaccgagg aggcggggtc ggaggcaggc aagatttcgt
      901 gcacgccgtg acaggaggag cagttggccg ccttcgtcat gccgtactcc agggcgatcc
      961 cgtgaaacga gtccatgtag gaggagaacc gcttcttcgg gagggcgtac cgggaggaga
     1021 ttccctcctt ttcatggcac ccggagcacg tcttggggat gttccgggcg gataccggcg
     1081 acgtcaggct gctgactttc gagatggtgt gctcaccgtg gcaatccgtg cacaccgggg
     1141 actccgcgat ccccgcgcgc atccccttcc cgtgaacgct cttttcgtac ttctcgagaa
     1201 tgcctgaatg acacttgccg cacagggtcg ggatattctg ccggaaggtg gcggacttgg
     1261 gatcgtcgcc gggaaggatg gtgtggttcc cgtggcaatc cgggcagacg gcgctgccgg
     1321 tgaggccgcc attcctcgac gccatcccgt ggacgctctg ctggtaggcg atcatcacct
     1381 gctggcccgg caggttgtac ttccgttcga tcgcctggtc cttgtggcac cgcaggcagg
     1441 tctccacgat gttgaggcgg aaggttcgcg ccgcggggtc cttcgacttg cggatgtcgt
     1501 gcttcccgtg gcagtcgtgg caccccggga cgtccgtgac gcccttcgcc cgcgccttcc
     1561 cgtgggagct catggcgtac gatttcgcgg cgtcgccgtg gcacctgccg cagtcgacct
     1621 tcgcgagctt ttcgtgcggc gccttcgcgt cggcgtggca ggtgacgcaa ccgctcgacc
     1681 cgtggaccga accgcggtac gcctggaggt cgacatggag cgagagcgtc tgcccgttgg
     1741 gctgtgtctt ctcgatcgtc ttgtccccgt ggcagtccat gcactcgctc acgctcgtgg
     1801 cggccccggc ggcaatcccg gccgacaaca gcaccgtggc ggcgaggacc gccgaaagat
     1861 agtggcggat catcctgttc attcctcctc cggatcgatc cgggttacgt cgggcggcgg
     1921 ggtctccccg cggcgcacgt acgcgtcgat ctcctcgagt tcgatggcgt gttcatgctc
     1981 catgtcgtgc cgggagagct tcccgtcgag ccaggtgaag gacatgggcg cgaaatccgg
     2041 gttcaggaac acgtagtaga ggtgccagac gatgattgcc agggtggcca ggatcgcctc
     2101 gtagaagtgg acgagggtgg ccacgtccag cccccacatc gggatatgct tcatcgcctc
     2161 ggtctggaac cagagcagga agccggtgac gaccatcacg atcgtgcccc ataccagcgc
     2221 gagatattcc gccttctcca cgtaactgaa ccggtcgaac ttcggcttgt ggtcggcgag
     2281 gccgatgtag taccggatcg tccccacgat gtcctccacg tccttccacc gcgggagcat
     2341 ccgccagacc tggccgcggc ccctggcggt gaagaccgcg tagaagacgt ggtagaggct
     2401 ggtcgcgatc atgaggacgg cggcgatgcg gtggcttccg ccgcgcagcg cgacgttggc
     2461 gtcgtcggag atcagcggga tcgaccattt gaacttcagg gcgaaccccg tgatcaccag
     2521 cgtgaagaag gtcgaaaggg tcaggatgtg ctgcgtccgc tcggagcggt tgagccgctc
     2581 gtatgcgggt tgccgccatt gccgccgctt gcggtagatc gcctgcatct tccggaaata
     2641 gtcgaaaccg ttgtggacga tcatcccgcc gatcaccacg acgatcatcc agatgtagag
     2701 ctgctccacc aagtacttga tcctgccccc cgtgccgccg gggcccacgt ggatgttccc
     2761 gctggcgaag ttcgtcgtgg cgccggggtg gcatttgccg caggtgacgg gaaggttctt
     2821 cgcgtagacc gtggagcgcg gatcggacga agggtagatc gcgtggtcgc cgtggcaaga
     2881 cgcgcagttc gccacggtct tgtcccccag gcgcgcggag aggccgtgga agctctgctg
     2941 gtacccgcgc accttgtcca ccggcacccc gtaccgggcg gccagcttct ctgcggcgtg
     3001 gcaggaggga caggtcttct gggaaatggc ggcggacgag acgggagagt tcgggtcgga
     3061 gacggcgcgt atgtcgtgct cgccgtggca atccgcgcag cttggggcgt cggggacgcc
     3121 gcgtgccagg gccgtcccgt gcacgctctc tttgtagagg gcgtagactc cgaagtggca
     3181 cctcccgcac gtcgcgggca ggttcgtctt gttgatggac gaggaagggt ctctccggtc
     3241 cttcatgttg tgggcgccgt ggcaatcgat gcagctggcg gacttgttca gccccatctc
     3301 gacgatggcc cggttgtgga tgctttgctg gtacagcttc gccgctcccg ggatggcgat
     3361 gttccggttg tcggtgatcg tcttgtcccc gtggcatcgg gtgcacgtca tggcgatgtt
     3421 ccggaagtag acgggagagg tggacacgga cgacttgggg acggcgtggg cgccgtggca
     3481 gtcgacgcac gcgggcgcgt tgccgatcgg attcttgctg aaggtgagga cgtggcggct
     3541 cttcgcgtag gcggccgcca gctcggagtg gcaaccggag cagtcgactc gcttgagtct
     3601 cgcgctatgg ggcacgctct tgatgtccgc gtggcagtcg gtgcaggata cgtccgcgtg
     3661 cacggactgc ttgtagagcc cgggatcgac gtagagggac atcccgggga aatttccccc
     3721 gacgtgcgga actcccttcg gccctcccgg cgtcacgttg gaggccttct ccgcgggaga
     3781 ccagccggga atcccctgat cgccgtggca gtcgaggcac tgcccgttgt ccagcgcgga
     3841 ggccgcgccg gcaagcagga gaaggatcgt caaggcgaag agcgccgtga ggaaggaccg
     3901 aagcgtacgc ctgcgcatta tcatcatcgc atccacccct tgttcgatgt cgcgggtcac
     3961 agagtcgcgg ggagtcagtc gagcctgttg tagactgtat acatacagca tcgatcctgg
     4021 cggaatcaag gaaaaaatgc ctcccggtcc ggcgacgccc aaggacgggc aagtgccgcg
     4081 ggagccagga atggcgggag cggccgggcg cagggatgca caggagcggc ccggagccag
     4141 gacgcggagg atggggaggt gccagcgagc cggacaggga cgtccggtga gcgtagcgag
     4201 ggtgggtgat cccacggaag gggaggcgga atcggcggaa aaccgtgcca cccgcaagat
     4261 gcctcgcgga acgcggccct gcggagaagc gttcctccgg cgggcgcgaa acatggcgtt
     4321 ttatttccgt ggaaccgtcc gcagaacgtg cagttatcga aatgtttctt tctcccccgc
     4381 tttttcttga cagcccggtg ctggcaccat atcttgtatt cgtcgcgcaa ccaaaccaca
     4441 agatatgcta ttctagcccg atacagcgac catgaaacgg agcgaatcga tggatctgaa
     4501 cgtcccagca accttcgcgt cacccgcatc ggccggcctg aacgacggcc ccgcgcaccc
     4561 cgccggaaag acccttcacg accatccgcc ccaggcgtgg gccgaggccc tcctgcggaa
     4621 acacggtccc atgccggtct cggagaacgc ccgaacggtg ctggaacgtc gctacctcaa
     4681 aggggggatg aagggggacc cgctggaaac ccccgaggag atggcgtgcc gcgtggcgta
     4741 caacatcgcg ctggcggaag gcctatacta cggcgccctc cccgaggtgg tgcttcggtg
     4801 ggcggaggcg ttctacgtga tgatgctccg gctggacttc ctccccaact ccccaacgct
     4861 gatgaacgcg ggccgcgagc ttcagcagct gtcggcgtgc ttcgtgctcc cggtggagga
     4921 ctcgatggag tcgatcttcg aggcggtcaa gaacaccgcc ctcatccaca agagcggcgg
     4981 ggggaccggc ttctccttct cccgcctgcg cccgcacgcc gacgtcgtcc gctcgacgaa
     5041 gggagtc
//
LOCUS       DASVJL010000042         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1038636, whole genome shotgun sequence.
ACCESSION   DASVJL010000042 DASVJL010000000
VERSION     DASVJL010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038636"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..532)
                     /locus_tag="VF357_00755"
     CDS             complement(143..532)
                     /locus_tag="VF357_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7522703.1"
                     /translation="MEDARYRIGLAAARNLDPEGPERLEAALREIAPDLYRHIVEFAF
                     GDILSRPGLDPKTREIATVSALTALGNAQPQLRFHLNAALNVGCTRAELAEVLMQMAV
                     YAGVPAALNALHLAKEVFAERDAKGLS"
     gene            complement(619..>2033)
                     /locus_tag="VF357_00760"
     CDS             complement(619..>2033)
                     /locus_tag="VF357_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX7522704.1"
                     /translation="FMEHDREEPTHRPVTERVGDYREIEELLPEDGIYRQAARCMDCG
                     IPFCHSYGCPVKNRIPDWNDMVYRKNWRKALDLLHATCNLPEITGRICPAPCEAACTL
                     TINLPAVTIRQLELQIVEHGWNEEWIRPEPAGFSTGKRVAVVGSGPAGLPAAQQLARS
                     GHEVVVFEKSDRIGGLLRYGIPDFKLEKWVIDRRLDQMRAEGVVFEKSVNAGVDVSAA
                     YLRRSFDAIVLAAGATAARDLPVPGRDLKGIHFAMEFLTQQNRRNAGDTIPGGEEISA
                     AGKHVVIIGGGDTGSDCIGTSRRQGAATITQIELLPKPPEDRLPTNPWPTWPVVLRTS
                     SSQEEGCERMWSVQTKELLGDQEGVRKLSCVKLDWSAPDAAGRSSFKEIPASAFELRA
                     DLVLLAMGFVHMEHGPLVRDLGVATDRRGNLVVGGECMTNVPGVFGAGDAVMGASLVV
                     RAINLGRLAAAGADRYLVGR"
BASE COUNT          320 a          707 c          652 g          354 t
ORIGIN      
        1 gccgccagga gcggaatgga gatcgtcttg cgcatcggtt ccctccgaaa ggatggtatg
       61 tggtatcgga tccctgaatc ggacccaaca tacatacccg caacgcgaca gggccgtcaa
      121 tcgcttcctg cggagcgacc ggtcaggaaa gccctttcgc atccctttcc gcgaagacct
      181 ccttcgcgag gtggagggca ttgagggcgg cgggaactcc cgcgtagacc gccatctgca
      241 tcaggacttc cgccaattcc gcgcgggtgc acccgacgtt gagggcggcg ttcagatgga
      301 accggagttg cggctgggcg ttgccgaggg ccgtcagcgc ggaaacggtc gcgatctccc
      361 gcgtcttcgg atccagtccg ggccgggaga ggatgtcgcc gaaagcgaac tccacgatgt
      421 gccgatacag gtcgggggcg atctccctca gagccgcctc cagccgttcc ggtccctccg
      481 ggtcgagatt ccttgccgcc gcgagcccga tccgatatct tgcgtcttcc acggttcccc
      541 ctccccgttc gatccgtatg ggtgatcgtc tgccaatgaa aatggagaga ttcccgggag
      601 gagcggagga ttcccgggtc agcgcccgac gaggtagcgg tccgcccctg cagcggccaa
      661 ccggccgaga ttgattgcgc gcaccacgag agaggccccc atcacggcat cccccgcgcc
      721 gaacacgccg gggacgttcg tcatgcactc cccgccgacg accaggttcc cccggcggtc
      781 cgttgcgacg cccaggtccc ggacgagcgg gccgtgctcc atgtggacga accccatcgc
      841 caggagcaca agatccgccc gcagctcgaa cgcggaggcg gggatctcct tgaacgaact
      901 tcttcccgct gcgtccggcg cggaccagtc gagcttcacg cacgaaagtt tccggacccc
      961 ttcttgatcg ccgaggagct ccttcgtctg gacgctccac atccgctcgc acccttcctc
     1021 ctgggaagaa gaggtccgca ggacgaccgg ccaagtgggc cacgggttgg tcggaagccg
     1081 gtcctccgga ggcttcggca atagctcgat ctgtgtgatc gtggcggccc cctgccggcg
     1141 gctggtgccg atgcagtcgg atcccgtgtc cccaccgccg atgatcacca cgtgcttccc
     1201 cgccgcggag atctcttccc ccccggggat cgtgtctccg gcgtttcgcc ggttctgctg
     1261 cgtgaggaat tccatcgcga agtggatccc cttgaggtcc ctccccggga cgggcaggtc
     1321 gcgtgccgcc gtcgccccgg cggccaggac gatcgcgtcg aaggatcggc gcaggtacgc
     1381 cgcggagacg tccacccccg cgttgacgct cttttcgaag accaccccct cggcccgcat
     1441 ctggtcgagt cgccggtcga tgacccactt ctcgagcttg aagtcgggga tgccgtaccg
     1501 caggagcccc ccgatccggt ccgatttctc gaagaccacc acctcgtgcc cgctgcgtgc
     1561 gagctgctgg gcggcgggga gtcccgcggg gcccgacccc acgacggcca cgcgctttcc
     1621 ggtcgaaaag cccgccggtt ccggccggat ccactcttcg ttccaaccgt gctccacgat
     1681 ctggagctcg agttggcgga tagtgaccgc ggggaggttg atcgtcaagg tgcacgcggc
     1741 ctcgcacggg gcgggacaga tccggcccgt gatctccgga aggttgcagg tggcgtgcag
     1801 caggtcgagc gccttccgcc agttcttccg gtagaccatg tcgttccagt cggggatccg
     1861 gttcttcacg gggcagccgt aggagtggca aaacgggatc ccgcaatcca tgcagcgcgc
     1921 cgcctggcga tagatcccgt cctcggggag cagctcctcg atctcgcggt agtccccgac
     1981 ccgctcggtc accggccggt gggtcggttc ctcccgatcg tgttccatga acc
//
LOCUS       DASVJL010000043         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_944437, whole genome shotgun sequence.
ACCESSION   DASVJL010000043 DASVJL010000000
VERSION     DASVJL010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944437"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VF357_00765"
     CDS             complement(<1..209)
                     /locus_tag="VF357_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEX7522705.1"
                     /translation="MVGNADLFVNWARKSSLWYLLFATACCGIELMQAGASRYDMDRF
                     GAVFRATPRQSDLMLVAGTITHKMA"
     gene            complement(287..682)
                     /locus_tag="VF357_00770"
     CDS             complement(287..682)
                     /locus_tag="VF357_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX7522706.1"
                     /translation="MLVEYATVLVFIVLGAVTVALMLGLSRWFAPSAPSAVKLSTYEC
                     GEVPYGSSWVQFNIRFYVVALIFIIFDVEVALLYPWAVVFQRLGLLAFIEAFIFIVIL
                     LAGLAYLWKEGDLEWVRTLQDAPVRKGAK"
     gene            1093..1896
                     /locus_tag="VF357_00775"
     CDS             1093..1896
                     /locus_tag="VF357_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HEX7522707.1"
                     /translation="MVPSLPTSPILLAPAGSLPAVEAALSAGADAVYIGVRSLSRGGR
                     TGLSPEAAGAAVSACRRAGARLHAAINAIPSFRELPAFLASLHRLRDAGIDEVILNDP
                     GVIALARREIPDWPICASVGLSVLNPEDARAYRDLGVDAVVLPSAVRPGEIPPIKKAS
                     GLRIEVFAYCRPEFLLHGKCGLTGYVAEGNGSGTASAKRGGSCRRVCRTLPVPAAVHS
                     LEDELPVWIAAGADVFKIEGRELSPPAVAALVTRFRRKLDAARAPSAGG"
     gene            complement(1882..>2350)
                     /locus_tag="VF357_00780"
     CDS             complement(1882..>2350)
                     /locus_tag="VF357_00780"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7522708.1"
                     /translation="PFDNRSGRSDPDGEMAPSLSHTIEIRVRFGETDPYGVAYFAAIL
                     DYFKRGMDEFLRSRGLSPDLAYRNRKRNYGFPVVATTCRYRAPVRFDDLLMLRTRVVR
                     VEGSGATFGFSLFRTEAGKDILAAEGRISCRSIDASWTPIPLPAELKNILSAR"
BASE COUNT          396 a          796 c          779 g          379 t
ORIGIN      
        1 tccgccatct tgtgggtgat cgtgccggcc accagcatca ggtccgactg ccggggggtg
       61 gcgcggaaga cggccccgaa ccggtccatg tcgtaccgcg acgcgcccgc ctgcatcagc
      121 tcgatgccgc agcacgccgt ggcgaacagg aggtaccaga gcgaggactt gcgcgcccag
      181 ttgacgaaca ggtccgcgtt tcccaccatg acgtgcgcct cgggcccgag ccccggggac
      241 ggggcgctcc cgtgcgcggg gggctggccg tgcgccgggt cgttactcat ttcgcgcctt
      301 tcctgaccgg ggcgtcctgc agggtgcgga cccattcgag gtccccctcc ttccaaaggt
      361 acgccagccc ggcgagcaga atcacgatga agatgaacgc ttcgatgaag gcgaggaggc
      421 ccagccgctg gaagaccacg gcccagggat acaggagcgc cacttcgacg tcgaagatga
      481 tgaagatgag cgccaccacg tagaaacgga tgttgaactg cacccacgag gagccgtacg
      541 gcacctcccc gcactcgtag gtggagagct tcaccgccga gggagcgctc ggggcaaacc
      601 agcgcgagag gccgagcatc agggcgacgg taaccgcccc cagcacgatg aacacgagga
      661 ccgttgcgta ttcgaccagc attgtctctt cttgtcccgc cctgggccgg aagatggcaa
      721 cgaaaccgcc ccacgacgcg ttttatttac ggcacgccga ggggaacggc tacatgcctc
      781 ggggatgcgg cttgcataca atcgacacta ccatactcag gaatacttcc aagtcaatca
      841 aaaaaaaatc ccgtccctcc atcgtgcgcc cgcgtcctac ccgcttctcc gcagggggcg
      901 gccccggtcg cagccggtcg ctgctccccg gctacacccg gcaaacggac tctgcgatca
      961 cctcccggtc ttctgacaat atctatcggt catagaaatt ttctcgcctt gaaatgcccg
     1021 gggtctccgc cgattccgca cgagtgacct gggtatgtcc cctgcccgta acccacaagc
     1081 ctataatgtc gtatggtccc gtcgctcccg acatcgccga tcctcctcgc gccggcgggg
     1141 tctctccccg cggtcgaggc ggccctgtcg gcgggggccg acgccgtcta catcggggtg
     1201 cgatccctct cccgcggagg ccggacaggg ctttccccgg aggcggccgg ggcggcggtt
     1261 tccgcctgcc ggcgggcggg ggcgcggctc cacgcagcga tcaacgccat cccgtcgttc
     1321 cgcgaactcc ccgccttcct ggcgtccctt catcgactcc gggacgcggg gatcgacgag
     1381 gtgatcctga acgatcccgg ggtgatcgcc ctcgcgcgac gggagatccc ggactggccg
     1441 atctgcgcct ccgtcgggct ctccgtcctc aaccccgagg atgcccgggc gtaccgggac
     1501 ctgggggtcg acgccgtcgt cctcccctcg gcggtccggc ccggggagat cccccctata
     1561 aagaaggcgt cggggcttcg gatcgaagtc ttcgcgtact gccgcccgga gttcctcctt
     1621 cacggcaagt gcggattgac gggatatgtt gcagagggga acgggagcgg gacggcttcc
     1681 gcgaaacggg ggggaagttg ccgccgcgtc tgccggaccc tgccggtccc ggcagcggtc
     1741 cactccctcg aggacgagct gccggtatgg atcgccgcgg gggcggacgt cttcaagatc
     1801 gaggggaggg aactttcccc gccggcggtg gcggcgctgg tcacccgctt ccggaggaag
     1861 ctggacgctg cacgcgcccc ctcagcgggc ggataagatg ttttttagtt ccgccggaag
     1921 agggatcggc gtccaggagg cgtcgatgga gcggcaggag atccgcccct cggcggcaag
     1981 gatgtccttc cccgcttccg tgcggaacag ggaaaagccg aacgtggcgc cgcttccttc
     2041 cacccgcacg acgcgagtgc gcagcatcag caggtcgtcg aaccggaccg gggcccggta
     2101 gcggcaggtg gtcgccacga cgggaaaccc gtagttccgc ttccggttcc ggtacgcgag
     2161 gtcgggggag agtcctcggg accggaggaa ctcgtccatc ccgcgcttga agtagtcgag
     2221 gatcgcggcg aagtacgcca cgccgtacgg gtcggtctcg ccgaaccgga cgcggatctc
     2281 gatcgtgtgc gaaagggaag gcgccatctc cccgtcaggg tcgcttctcc ctgaccggtt
     2341 gtcaaacggg
//
LOCUS       DASVJL010000044         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1695155, whole genome shotgun sequence.
ACCESSION   DASVJL010000044 DASVJL010000000
VERSION     DASVJL010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695155"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /gene="lpxC"
                     /locus_tag="VF357_00785"
     CDS             <1..1132
                     /gene="lpxC"
                     /locus_tag="VF357_00785"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX7522709.1"
                     /translation="PGMDGIETLRRMKAAQPETPVIMMSGHATISTAIKATKVGASDF
                     IEKPLELDVVLNAIRRALGTQDVVRSSASGELAGSIDLRSPEGMPELQPLVFGNQTLR
                     GARMPQRTLARSAVLYGQGLHSGKKSGLIFEPLGPNSGIHFVGVSDNRAIPAHLDFVE
                     STGYATTIRLGTTHVATIEHVMSALNAYGVSNLLIKCNGEVPVLDGSSVEFCSLFEEV
                     GFENQIGDWHGILVKEPVRIDAGKASIRLEPCDAFEIDYTLEYPAPVGMQRFVFRLDD
                     PATYRKEIAPARTFGFARDIGLLQRQGLALGGRFDNFVLFGEEGPSNDALRFPDEPVR
                     HKIMDMIGDLYLLGRRLQARVVAHMTGHTQNIAVLKKVRELL"
     gene            1161..2042
                     /locus_tag="VF357_00790"
     CDS             1161..2042
                     /locus_tag="VF357_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7522710.1"
                     /translation="MREIVAEEGSPFLIRWRKTFSGCAAAVLLVLLVPGSRSAAAEMI
                     RISGTGGAIGTMRILGEAFRTANPGIRVEILPGIGSSGSVKAVMAGRLDIGLSARTLS
                     GEERTKGVMETKYARTPFVFCVNSTLKMTGLTPEGIAAIYSGKSDWENGRRIRLVLRP
                     PKDTDIPILKSMSPAMSAAVDIAMRRQAMIVAMTDDDAADAIEDVPGAFGGTTLSLVL
                     SEKRVLRVLSLDGVMPSVQTMADRSYPYGKTFFMVTKNNPSASVRRFIDFVRSPAGAS
                     ILAKNGQEAFREDGVLP"
     gene            2039..>2611
                     /locus_tag="VF357_00795"
     CDS             2039..>2611
                     /locus_tag="VF357_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522711.1"
                     /translation="MKNTGRNIPRLIEGFGGVLLLILGVALPVLYFVNRLELESEHLR
                     TETAIYARLVSSVITRNPDLWMYETTRLEGLLAKGPEDNRVESVRILDEDGTMVMRRE
                     ERLAWPLMTRTHPLMDSDMIVGSLEESTSLRPLIVETSILAMAGTGITALLFFLFRTY
                     PIAALRNALDLLSREKGRAKVTLQSIGDGVI"
BASE COUNT          497 a          794 c          844 g          476 t
ORIGIN      
        1 gccggggatg gacgggatcg agacgctccg gcggatgaag gcggcacaac cggaaacgcc
       61 cgtgatcatg atgagcgggc acgcgacgat ttcgaccgca atcaaggcga ccaaggtcgg
      121 cgcctcggat ttcatcgaaa aaccgctcga actcgatgtc gtgctgaacg cgattcgtcg
      181 ggccctgggg acccaggatg tcgtccggag ctccgcctcg ggcgaactcg ccggttcgat
      241 cgatctccgg tcaccggagg ggatgccgga actgcaaccc ctggtgttcg ggaaccagac
      301 gctgcgcgga gcacgcatgc cgcagaggac tctcgcccgc agcgcggtcc tgtacgggca
      361 ggggcttcac tccgggaaga agagcggcct gatcttcgag ccgctgggtc ccaattcggg
      421 aatccacttc gtcggggtct ccgacaaccg ggcgattccc gcgcaccttg atttcgtcga
      481 gtcgaccgga tatgccacga cgattcgcct cggaaccacc catgtcgcaa ccatcgagca
      541 tgtcatgtcc gcgctcaacg cctacggggt cagcaatctg ctcatcaagt gcaacggcga
      601 ggtccccgtg ctggacggtt cgtccgtgga gttctgttcc ctgttcgagg aagtcggttt
      661 cgagaaccag atcggggact ggcacgggat cctggtgaag gaacctgttc ggatcgacgc
      721 aggcaaggca tcgatccggc ttgaaccatg cgacgccttc gaaatcgact acacgctcga
      781 atatccggcc ccggtaggca tgcagcgctt cgtcttccgt ctcgacgacc ccgcgaccta
      841 tcggaaggag atcgccccgg cgcggacctt cgggttcgcg cgggacatcg ggctattgca
      901 gcggcagggg ttggctctcg gcgggcgctt cgacaacttc gtcctcttcg gggaggaggg
      961 accgagcaac gacgcccttc gattcccgga tgaaccggtg cgtcacaaga tcatggacat
     1021 gatcggggat ctctacctcc tcggccggcg tttgcaggcg agggtggtcg cccacatgac
     1081 gggccacacg cagaacatcg ccgtactgaa gaaagtgcgg gagttgctgt gactcacgat
     1141 acgactctgg gtgtcccctg ttgcgggaaa tcgttgcaga ggaagggagc ccgtttctga
     1201 tccggtggcg gaagacattt tccggttgtg cggccgccgt cctgctggtg ctcctcgtcc
     1261 cggggtcccg aagtgcggcc gcggagatga tccggatctc cggaaccggc ggcgcgatcg
     1321 ggacgatgcg gatcctcggg gaggcgttcc ggacagccaa cccggggatt cgggtcgaaa
     1381 tattgccggg catagggagc agcggaagcg tcaaggccgt catggcggga cgtctggaca
     1441 tcggattgag cgcacgaacg ttgagcggcg aggaacgcac caaaggcgtc atggaaacga
     1501 aatacgcgag gactccattc gtgttctgcg tcaacagcac cctcaagatg accggcctga
     1561 ccccggaagg catcgccgcg atctactccg ggaagagcga ttgggagaac ggaaggcgga
     1621 tccggctggt cctccgtcca ccgaaggata cggatatccc gatcctgaag agcatgtccc
     1681 cggcgatgag cgcggcagtc gacatcgcga tgcgccgcca ggcgatgatc gtggccatga
     1741 cggacgatga tgcggccgac gccatcgaag acgtgccggg cgccttcggg gggacgacgc
     1801 tttcgcttgt cctatccgag aagcgcgtgc tccgcgtcct atcgctggat ggggtaatgc
     1861 cgagcgtcca gaccatggcc gacagatcgt acccttacgg caagacgttc ttcatggtca
     1921 cgaagaataa tccctccgct tcggttcgcc ggttcatcga tttcgtccgc tctcccgcgg
     1981 gagcgtccat ccttgcgaag aacggccagg aagcattccg ggaagacggc gtccttccat
     2041 gaagaatacc gggcggaaca tcccccggct catcgaggga ttcggcgggg ttctgctgct
     2101 gatcctcggg gtggccttgc cggtgctcta cttcgtgaac aggttggagt tggagagtga
     2161 acatctccgg acggaaacgg cgatctatgc ccggctggtg tcctccgtga tcaccaggaa
     2221 tccggacctg tggatgtacg aaacgacccg actcgagggt ctccttgcga aaggacccga
     2281 agacaatcgt gtggaatccg tccgcatcct ggacgaggac gggaccatgg tgatgcggcg
     2341 ggaggagcgt ctcgcctggc cgctcatgac acgaacgcac cccctgatgg attccgacat
     2401 gatcgtggga tccctcgagg agagcacctc ccttcgcccg ctgatcgtcg aaacttccat
     2461 cctggccatg gccggaacgg ggatcaccgc gctcctgttc tttctcttcc ggacttatcc
     2521 gatcgccgcg ctccgcaacg cccttgactt gttatcgcgc gagaaggggc gcgccaaggt
     2581 caccctccaa tcgatcgggg acggtgtcat c
//
LOCUS       DASVJL010000045         3809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_569715, whole genome shotgun sequence.
ACCESSION   DASVJL010000045 DASVJL010000000
VERSION     DASVJL010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569715"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VF357_00800"
     CDS             complement(<1..177)
                     /locus_tag="VF357_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX7522712.1"
                     /translation="MAEVTLIKAVNDALATEMARDPSVCVLGEDVGRDGGVFRATEGL
                     LRRFGPERVIDTPLN"
     gene            complement(182..1252)
                     /locus_tag="VF357_00805"
     CDS             complement(182..1252)
                     /locus_tag="VF357_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX7522713.1"
                     /translation="MTGPEILSVLRPDGTVDPSLDPGLPDPEMLSLYRHMVLLRAVDE
                     KAVALQRSGRIAFFVPSTGQEACSIGAGHAVREGDWIFPAYRDQGVGLMRGYPLAALF
                     GQIVGHTTDTTRGRQMPNHWCDRSIRLVSVSSPVATQLPHAVGAAYAAKVRGERIAVL
                     TTFGDGATSTGDFHAAMNFTGVFRLPVVFFCENNRYAISHPVERQTATGTLAEKGPAY
                     GLPGVRVDGNDVLAVRKAVGDAMDRARAGEGGTLIEALTYRIGPHSTSDDPTVYRSEE
                     EVAAWRGKDPIRRFAAFLKGRGLLEDPAAEEAEAKEIVRSAAEAAMGAPPVPLSSMFE
                     DVYAEIPWHLAEQRDSLLSGKG"
     gene            complement(1249..2481)
                     /locus_tag="VF357_00810"
     CDS             complement(1249..2481)
                     /locus_tag="VF357_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX7522714.1"
                     /translation="MTLLSGIRALDLSLQLPGPFCTMMMADYGADVVKIDEPSPRVRN
                     PFAAEEPGTGPVDRYQNRGKRSVTINLKTEEGKAIFLRLVETADVVVEGFRPGVVRRL
                     GIDYDAVSAVNPMIVYCSISGYGQDGPMRDVPGHDVNYLSYAGVLGLCGRKGDDPTLL
                     PVQVGDVFGGSMMALSGILMALLGRERTGKGGRVDVSMTDGAMASLALPAASWLGGGV
                     PQERGSLPLTGMFPCYEVYRCADGGFLSVGALEAWFWKALTSALGREELAGMQYATGA
                     EGERVKEELRAVFLTLPRDEWVKRLAGIDACVSPVLDLPEALLHPNTASRRMVVDVES
                     PLGGIDRQLGMPIKIDGVLGAPRRAPLLGEHDDEILTGLGYSASLIADLRAKGVIRKR
                     KGVGGQLPSADGRKGETS"
     gene            2527..3297
                     /locus_tag="VF357_00815"
     CDS             2527..3297
                     /locus_tag="VF357_00815"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX7522715.1"
                     /translation="MEKGFPISPSRAIFLADAHLNQEDIHSRNFLALADRAAEEKVPL
                     FLLGDVFDLWFGNAGLTFAFQKPIIEHLRKLRRGGLRLYYVEGNRDFYLKREHEGTTF
                     DVVSEGEMHVAVGKKRLVLSHGDTVNRADFTYRFWKGISKSRFANEAVAHLPPSIILP
                     MAEGIERNLKRSNRRHRGMFPERECREYAMRLFREGVDFAILGHFHQERLSRFSKEES
                     TKVVAVLPSWKDQWRYFYLTAEGAHGFRAFKPDEPLLP"
     gene            complement(3269..3691)
                     /locus_tag="VF357_00820"
     CDS             complement(3269..3691)
                     /locus_tag="VF357_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522716.1"
                     /translation="MGEVFRRIARRYFRAALFYLALGLLLGAGMLWFGNDNFQFLHSH
                     MLLVGTGVFTAYGAGYWWVAGRSDAELPPGVTPTLASAQFFLANVGLPGMLAGSVLPV
                     GLGLDRIGILFGFIEAAAGILFALLLSRALKAAAARRA"
BASE COUNT          663 a         1364 c         1197 g          585 t
ORIGIN      
        1 gttcagcggc gtgtcgatca cccgctccgg cccgaaccgg cggagcaatc cttccgtcgc
       61 ccggaaaacc ccgccgtcgc ggccgacgtc ctccccgagg acgcagacgg acggatcccg
      121 cgccatttcc gtcgcgagcg cgtcgttgac cgccttgatc agcgtcactt cggccatcgc
      181 ctcatccctt tccggagagg agggagtcgc gctgctcggc gaggtgccat ggaatttcgg
      241 cgtagacatc ctcgaacatg ctggacagcg gcacgggggg cgctcccatc gccgcctcgg
      301 ccgcgctgcg tacgatctcc ttcgcctccg cctcctccgc ggccgggtcc tcgagcaggc
      361 cccgcccctt gaggaacgcg gcgaaccgcc ggataggatc ctttcccctc cacgcggcca
      421 cctcctcttc cgaccggtag accgtcgggt cgtcggaggt cgagtgcgga ccgatgcgat
      481 acgtgagcgc ttcgatcaac gtgcccccct ccccggcccg cgcccggtcc atcgcgtctc
      541 cgaccgcctt gcggaccgcc agcacgtcgt tgccgtcgac gcggacaccg ggaagtccgt
      601 acgccgggcc cttctcggcc aacgtcccgg tggccgtctg gcgctccacg ggatgggaga
      661 tggcgtaccg gttgttctcg cagaaaaaga ccaccggcag ccggaagacc ccggtgaagt
      721 tcatcgccgc gtggaaatcc cccgtggagg tcgcgccgtc gccgaaggtg gtgagcacgg
      781 cgatccgctc cccgcgcacc ttcgccgcgt acgccgcccc gacggcatgg ggaagctgcg
      841 tggccaccgg ggaggagacc gacaccaggc ggatggagcg gtcgcaccaa tggttcggca
      901 tctgccgccc ccgcgtcgtg tccgtcgtat gcccgacgat ctgaccgaac agggcggcga
      961 gcgggtaccc ccgcatcagc cccacccctt ggtcccggta cgccgggaag atccagtccc
     1021 cctcgcgcac ggcgtgtccc gccccgatcg aacacgcctc ctggccggtc gacgggacga
     1081 agaaggcgat ccggccggac cgctggaggg cgaccgcctt ctcgtcgacc gcccgcagca
     1141 gcaccatgtg ccggtagaga gacagcatct ccggatcggg caaccccgga tcgagcgaag
     1201 ggtctaccgt tccgtccgga cgaaggaccg acaagatttc cgggcccgtc atgacgtttc
     1261 ccccttccgc ccgtcggcgg aaggcagctg ccccccgaca cctttccgct tccggatcac
     1321 gcccttcgcg cgcaggtcgg cgatcagcga agccgagtac cccagcccgg tcaggatttc
     1381 gtcgtcgtgt tccccgagga gcggcgcccg acgaggcgcc cccaggaccc cgtcgatctt
     1441 gatcggcatc ccgagctggc ggtcgattcc gccgagaggg gactcgacgt cgacgaccat
     1501 gcggcgcgac gccgtgttcg gatgaagcaa cgcctccggg agatccagga cgggagatac
     1561 gcaggcgtcg attccggcaa gccgcttcac ccactcgtcc cgggggaggg tgagaaacac
     1621 ggctcgcagc tcttccttga cgcgttcccc ctccgcgccg gtcgcgtact gcatcccggc
     1681 cagctcctcg cgcccgagcg cggacgtcag cgccttccag aaccaggcct cgagcgcccc
     1741 gacactgagg aagccgccgt ccgcgcaccg gtacacctcg tagcagggga acatcccggt
     1801 caacggcagg gagccgcgct cctgcggcac accgccgccg agccaggagg ccgcaggcaa
     1861 tgcgagggat gccatcgccc cgtccgtcat cgagacgtcc acccgaccgc ccttgccggt
     1921 ccgctcccgc ccaagaagcg ccatgaggat cccggagagc gccatcatgg agccgccgaa
     1981 cacgtccccc acctgcaccg gaagaagcgt cgggtcgtcg cctttcctcc cgcacagtcc
     2041 caacaccccc gcgtaggaca ggtaattgac gtcgtggccc gggacgtccc gcatcgggcc
     2101 gtcctgaccg tacccggaga tggagcagta aacgatcatc gggttgacgg cggagaccgc
     2161 atcgtagtcg atgccgagcc gccggacaac gcccggccgg aacccctcca ccacgacgtc
     2221 ggcggtttca accaggcgca ggaagatcgc cttcccctcc tccgtcttca ggttgatcgt
     2281 cacgctccgc ttcccccggt tctgataccg gtcgaccggt cccgtccccg gctcttcggc
     2341 cgcgaacggg ttgcgcaccc gcgggctcgg ctcgtcgatc ttcacgacgt ccgccccgta
     2401 gtccgccatc atcatcgtgc aaaacggccc cggaagctgg agcgacaggt ccaacgcgcg
     2461 tatcccggag agcagcgtca tcccgaatcc cccctagaaa tctcttgtgc tatcttaata
     2521 tctcctatgg aaaaagggtt tccgatctct ccgagccggg cgatcttcct cgcggacgcc
     2581 cacctgaacc aggaagacat ccacagccgg aacttccttg ccctcgccga ccgggcggcg
     2641 gaagaaaagg tgcccctgtt cctcctcggg gacgtgttcg acctctggtt cggcaacgcc
     2701 gggctcacct tcgccttcca gaaaccgatt atcgaacatt tacggaaatt gcggcggggg
     2761 ggattacgcc tgtactacgt ggaggggaac cgcgacttct acctgaagcg ggaacacgag
     2821 gggacgacgt tcgacgtcgt ttcggaggga gagatgcacg tggcagtcgg gaagaagcgg
     2881 ctggtcctgt ctcacgggga cacggtgaac cgcgcggatt tcacctaccg gttctggaag
     2941 ggaatctcga agagccgctt cgccaacgaa gcggtcgccc acctgcctcc ctcgatcatc
     3001 cttcccatgg cggagggaat cgagcggaac ctgaagcgtt ccaatcggag gcacagggga
     3061 atgtttcctg agagggagtg ccgggaatac gcgatgcgcc tctttcgcga aggggtcgac
     3121 ttcgcaatcc tcggacactt ccaccaggag cggctgagcc gtttttccaa ggaggagtcg
     3181 acgaaggtcg tcgcggtcct gccgtcgtgg aaggaccagt ggcgctactt ctacctcacg
     3241 gccgaggggg cgcacggctt ccgcgcgttc aagcccgacg agccgctgct gccttgaggg
     3301 cgcgggaaag aagaagggcg aagaggatcc ccgcggccgc ttcgatgaag ccgaagagga
     3361 tgccgatccg gtcgagcccg agcccgaccg gcagcaccga acccgccagc atgccgggaa
     3421 gaccgacgtt ggcgaggaaa aactgcgcgc tggccagggt cggggtgacg ccgggcggca
     3481 gttccgcgtc cgaccgcccg gcgacccacc aataccccgc gccgtaggcg gtgaagaccc
     3541 ccgtccccac cagcagcatg tgggagtgaa gaaactggaa gttgtcgttc ccgaaccaga
     3601 gcatcccggc gcccaggaga agcccgaggg cgaggtagaa cagggccgcg cggaaatacc
     3661 tccgcgcaat gcgccggaac acctcgccca cgctacttcg ccgtcccgtt ttcgatggag
     3721 aacgtggacg ccgccagtgc cgggtccgcg tggaacgcca ctttcccgct gcacagcttc
     3781 tcgagccggt tgatgtcgtc ccgcttctg
//
LOCUS       DASVJL010000046         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_757220, whole genome shotgun sequence.
ACCESSION   DASVJL010000046 DASVJL010000000
VERSION     DASVJL010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757220"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..657)
                     /gene="rdgB"
                     /locus_tag="VF357_00825"
     CDS             complement(49..657)
                     /gene="rdgB"
                     /locus_tag="VF357_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX7522717.1"
                     /translation="MRLLIASKNRGKVLEIRALLGLALQKVVEVVTLAEMPAVEQPRE
                     DGKTFAENARIKALHYAKAHKILCIADDSGLAVEPLGGSPGVRSARYAGEGASDAANN
                     AHLLHELEPFARPWKAAYVCVAVTALPQRVVAEATGKMEGEILPAGRGRDGFGYDPLF
                     FVPSLGKTMAELSTEEKNRISHRGQALRALIAEMKVAGLLLS"
     gene            complement(654..1388)
                     /gene="rph"
                     /locus_tag="VF357_00830"
     CDS             complement(654..1388)
                     /gene="rph"
                     /locus_tag="VF357_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX7522718.1"
                     /translation="MTFKRADGRKSLDIRTIGASLGVQKHAEGSVLFTMGDTRVVCAA
                     TIEERVPTFLRGAGKGWVTAEYSMLPRATHTRVAREGRTGKVGGRTHEIQRLVGRSLR
                     AVVDFEALGERTVTIDCDVLEADGGTRTASINGAWIALWQACRRLVAKGVVSRNPVRD
                     HVVAVSVGIVQGRILADLDYSEDSAAEVDMNVVMTGNGRLIEVQGTAEREPFTRERLD
                     AMLSAAAAAGRKILKVQRRFAERGSR"
BASE COUNT          223 a          581 c          459 g          249 t
ORIGIN      
        1 gcaacgatgc accggactcg cggagtgtcg tcgaaccggc cccgggccct acgagagcag
       61 cagccccgcg accttcatct ccgcgatcag cgcccgcagg gcctggccgc ggtggctgat
      121 ccggttcttc tcttccgtcg aaagctccgc catcgtcttt ccgagggaag ggacgaagaa
      181 gagcgggtcg tatccgaagc cgtcgcggcc ccgtcccgcg gggaggatct ccccctccat
      241 ttttcccgtc gcctccgcga ccacccgttg cgggagcgcg gtcaccgcca cgcagacgta
      301 cgccgccttc catgggcgcg cgaacggctc cagctcatgg agcagatgcg cgttgttcgc
      361 cgcgtcggag gccccttccc cggcgtaccg ggcggagcgc acgccgggag accccccgag
      421 cggctccacc gcaagacccg agtcgtcggc gatgcacagg atcttgtgcg ccttggcgta
      481 gtgaagcgcc ttgatccgcg cgttttcggc gaacgtcttc ccgtcctccc gtggctgttc
      541 cacggcgggc atctccgcca gcgtgacgac ctcgacgacc ttctgcagcg cgagcccgag
      601 gagcgcccgg atctccagaa ctttcccccg gttcttcgat gcgatcagaa gcctcatcgc
      661 gacccccgtt ccgcgaagcg acgctgcacc ttgagaatct ttctccccgc ggccgccgcg
      721 gccgagagca tcgcgtcgag ccgctcccgg gtgaacggct ccctctccgc cgtcccctgg
      781 acctcgatca gccggccgtt ccccgtcatc accacgttca tatccacttc cgccgcggag
      841 tcctcggagt agtcgaggtc tgccaggatc cgcccctgga cgattcccac gctgactgcc
      901 accacgtggt cgcgcaccgg attgcgggag accaccccct tggcgaccaa cctccggcac
      961 gcctgccaca gggcgatcca cgccccgttg atggaggcgg tccgcgtccc cccgtcggcc
     1021 tccaggacgt cgcagtcgat cgtcacggtg cgctcgccga gcgcttcgaa gtcgaccaca
     1081 gcccgcagcg accgccccac cagccgctgg atctcatgcg tgcggcctcc cactttcccc
     1141 gttcgacctt cccgggcgac ccgggtgtgc gtcgcgcgcg gaagcatcga gtactcggcg
     1201 gtgacccacc ccttccccgc gccgcgcagg aacgtgggga cccgctcctc gatcgtcgcc
     1261 gcgcacacca cgcgggtgtc ccccatcgtg aaaaggacgg acccttcggc atgcttctgc
     1321 accccgaggg aggcgccgat cgtccgaatg tccagagact tgcgcccgtc ggcgcgcttg
     1381 aatgtcatca ctcctcctgc ccgcggtgca ttataccttg tccagcacct cccgaacgcg
     1441 tcgggcgagg gaggccgggc tgtatggctt cgggaggaac tcgatcccgg gcaggaggac
     1501 gaacgactcg tg
//
LOCUS       DASVJL010000047         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_476011, whole genome shotgun sequence.
ACCESSION   DASVJL010000047 DASVJL010000000
VERSION     DASVJL010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476011"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VF357_00835"
     CDS             <1..543
                     /locus_tag="VF357_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522719.1"
                     /translation="GVPAALGFSEASGIPFEMGLIRNHYVGRTFIEPQQSIRHFGVKI
                     KLNAVRGVVSGKRVAVVDDSIVRGTTGRKIIKMIRAAGAKEVHFRISSPPTCYPCFYG
                     IDTPLRRDLIAATHTREETNTYLTSDSIGYLSMEGLHACVPNGNTTYCDACFSGNYSV
                     PLGTEESGEQLPLFRGSVRV"
     gene            586..1182
                     /gene="pyrE"
                     /locus_tag="VF357_00840"
     CDS             586..1182
                     /gene="pyrE"
                     /locus_tag="VF357_00840"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX7522720.1"
                     /translation="MPSATDRSAVFPPEMTADRRRFLALLREKSYERRKVVLSSGRES
                     DFYIDCRQSTLDAEGAVLTGRLFCAMLERGERPEAVGGITLGADPIVTAVSLTSALRG
                     WPVPAFIIRKEPKKHGTEQWIEGTKNLRPGMRVAILEDVVTTGASTMRAIERAVGAGL
                     IVARVLCLVDRNEGGSEAVAAVGYRVESMFLREDVEHG"
     gene            1175..1804
                     /locus_tag="VF357_00845"
     CDS             1175..1804
                     /locus_tag="VF357_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522721.1"
                     /translation="MADNARRASMAAALLALAAVAAGCGPRSVALYEKVMGSPSYGRV
                     TESATRTREVHDGLDTRFILSSTWLSGPWVSAFAEEYSNIYYLDAERRERVVSHWRGE
                     SEKYVRFFVALYVPEEKGNDLEKPGTLWSLRLVRADEKDFEPVYIHKSSLRTEEISRF
                     FPYSGTWYHAYEVAFPREAGELAGSPRAGSPRLKLVLSGVEGRAVLAWQ"
     gene            complement(1834..>3278)
                     /gene="nadB"
                     /locus_tag="VF357_00850"
     CDS             complement(1834..>3278)
                     /gene="nadB"
                     /locus_tag="VF357_00850"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF006567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX7522722.1"
                     /translation="EDTFESHIEDTHKPGAGLCHADTVDLVVRDAPARIRELIEWGVE
                     FTRQTGKREFDLHREGGHSRRRIFHAKDLTGREVERALLVRARENPRIQIFENHTAIN
                     LITRQKLSSFDHPGVDEVLGAYVLDRKTGAIHTFVADVTVLATGGSGKVYLYTSNPDV
                     ATGDGVALAYRAGATIANMEFVQFHPTCLYHPRAKSFLISEAVRGEGAVLLNRAGKRF
                     MEGAHPMRELAPRDIVARAIDAEMKHTGEDCVYLDIRFKGPAFIRKHFPNIHGTCLSF
                     GIDMTKEPIPVVPAAHYQCGGVRTNLHGETDIQRLFALGECAGTGLHGANRLASNSLI
                     EALVFSHSAVQRASTAYIGKPRPRIDIPKWDPGKAQEPDEAVVVSHNWEEIRRTMWNY
                     VGIVRSNKRLSRALDRIELLKREISEYYWNFKVTGDLLELRNICTVAELIVRCAMQRK
                     ESRGLHTTIDYPYLDDEHGRKDTLVRRTRF"
BASE COUNT          511 a         1078 c         1120 g          569 t
ORIGIN      
        1 ggcgtccccg cggcgctggg attctccgag gcgtccggga tccccttcga gatgggcctc
       61 atccggaacc actacgtagg gcgcaccttc atcgagccgc agcagtcgat ccgtcacttc
      121 ggcgtgaaga tcaagctgaa cgccgtgcgc ggagtcgtct ctgggaaacg ggtcgcggtg
      181 gtggacgact ccatcgtccg ggggacgacc gggcgcaaga tcataaagat gatccgggcg
      241 gccggggcga aagaggtcca cttccgcatc agttcccccc cgacgtgcta cccgtgcttc
      301 tacgggatcg acaccccgct gcggcgggac ctgatcgccg cgacccacac gagggaggag
      361 acgaacacct acctcacctc cgacagcatt ggatacctca gcatggaggg cctccacgcc
      421 tgcgtgccaa acgggaatac gacctactgc gacgcctgct tctcggggaa ctactcggtt
      481 ccgctcggaa cggaagagtc cggggagcag ttgccgctgt tccgcggctc ggtccgggtc
      541 tgacggaccg ggaaaagccg agagatcacc catccggggg aaacgatgcc cagcgcgacc
      601 gatcgatctg ccgtgttccc gccggagatg accgccgacc ggcggcgctt cctggccctc
      661 ctgagggaga aaagctacga gcgccgcaag gtcgtccttt cctcgggacg ggaatccgac
      721 ttctacatcg actgcaggca gagcacgctt gacgcggagg gggcggtcct cacggggcgg
      781 ctcttctgcg cgatgctcga gcggggggag cgcccggagg cggtgggggg gatcaccctc
      841 ggggcggatc cgatcgtgac cgccgtctcc ctcaccagcg ccctgcgggg atggccggtg
      901 cccgcgttca tcatccgcaa ggagcccaag aagcacggaa cggagcagtg gatcgagggg
      961 acgaagaatc ttcgtcccgg gatgcgcgtg gcgatcctgg aggacgtggt gaccaccggc
     1021 gcttcgacga tgcgggcgat cgagcgcgcc gtcggggcgg gactgatcgt ggctcgggtc
     1081 ctttgcctcg tcgaccggaa cgagggcggt tcggaggcgg tggcggcggt cgggtaccgc
     1141 gtcgagtcga tgttccttcg ggaggacgtg gaacatggct gacaacgcgc ggcgcgcgtc
     1201 aatggcggcg gcgctcctgg ctctcgcggc ggtcgcggcc ggctgcggtc cgcggtcggt
     1261 cgccctctac gagaaggtga tgggatcccc ctcctatgga cgggtcacgg agtccgcgac
     1321 ccgcacccgc gaggtccacg acgggctgga cacccgattc atcctgtcct ccacctggct
     1381 ctcgggaccg tgggtctcgg ccttcgcgga agagtattcg aatatctact acctcgacgc
     1441 ggagcggcgg gagcgggtgg tctcccattg gcgcggggaa tcggagaaat acgttcgctt
     1501 cttcgtcgcc ctctacgtcc cggaggagaa ggggaacgac ctggagaaac ccgggacgct
     1561 ctggagcctg cggctcgtgc gggcggacga gaaggatttc gagcccgtct acatccacaa
     1621 gtcctccctg cgtacggagg agatctcccg cttcttcccg tactcgggga cgtggtacca
     1681 cgcgtacgag gtggcgtttc cgcgggaggc gggtgagctc gcggggtcgc cgcgggccgg
     1741 gtccccgcgc ctgaagctgg tcctgtcggg cgtggaaggg cgcgccgtcc tcgcctggca
     1801 gtaaggaggc taacctcctg acccgaagat cgcctaaaac cgggtgcggc ggaccagcgt
     1861 gtccttgcgc ccgtgctcgt cgtccaggta tgggtagtcg atcgtcgtgt ggagcccccg
     1921 gctctccttg cgctgcatgg cgcaccggac gatcagctcc gccactgtgc agatgttgcg
     1981 aagctcgagc aggtcgcccg tcaccttgaa gttccagtaa tactccgaga tctcccgctt
     2041 cagcagctcg atccggtcga gcgcgcggga cagccgcttg ttcgaccgca cgatccccac
     2101 gtagttccac atcgtccggc ggatctcctc ccagttgtgg gagaccacga ccgcctcgtc
     2161 cggttcctgc gccttgcccg ggtcccactt cgggatgtcg atcctgggcc tcggtttgcc
     2221 gatgtacgcg gtagaggcgc gctggaccgc gctgtgcgag aagaccagcg cctcgatcag
     2281 ggagttcgag gcgaggcggt tcgcgccgtg gagccccgtg cccgcgcatt ctcccagggc
     2341 gaagagccgc tggatgtccg tctccccgtg gagattcgtc cgcacgccgc cgcactggta
     2401 gtgcgccgcc gggaccaccg ggatcggctc cttcgtcatg tcgatgccga acgaaaggca
     2461 cgtcccgtgg atgttcggga agtgcttcct gatgaacgcc ggacccttga accggatgtc
     2521 gagatagacg caatcctccc cggtgtgctt catctccgcg tcgatcgccc gcgcgacgat
     2581 gtccctcggg gcgagctccc tcatcgggtg ggcgccttcc atgaaccgct tcccggcgcg
     2641 gttcaggagg accgccccct cgccgcggac cgcctcggag atgaggaacg acttggcgcg
     2701 cgggtggtag aggcaggtgg ggtggaactg gacgaattcc atgttggcga tcgtcgcccc
     2761 cgcgcggtac gccagcgcga ctccgtcccc cgtggcgacg tcggggttgc tcgtgtacag
     2821 gtacaccttt cccgaaccgc cggtggcgag gaccgtcacg tccgccacga aggtgtggat
     2881 cgcgccggtc ttcctgtcca gcacataggc gccgagcacc tcgtcgaccc ccggatggtc
     2941 gaaagaggag agcttctgac gcgtgatgag gttgatcgcc gtgtgattct cgaagatttg
     3001 gatccggggg ttctcccggg cgcggacgag caacgcccgt tccacctctc gcccggtcag
     3061 gtccttcgcg tggaagatcc tccggcgcga gtgtcccccc tcgcggtgga ggtcgaactc
     3121 cctcttgccg gtctgccggg tgaactccac cccccactcg atcagttcgc gtatccgcgc
     3181 cggggcatcc cgcaccacga ggtcgaccgt gtcggcgtgg caaagccccg cccccggctt
     3241 atgggtatcc tcgatgtggg actcgaaggt gtcctcgt
//
LOCUS       DASVJL010000048         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1601399, whole genome shotgun sequence.
ACCESSION   DASVJL010000048 DASVJL010000000
VERSION     DASVJL010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601399"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1580)
                     /locus_tag="VF357_00855"
     CDS             complement(105..1580)
                     /locus_tag="VF357_00855"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX7522723.1"
                     /translation="MTPIPFDPTALVQGLYSILPELVVIATAIAVLLADLVLPERGKK
                     SLCHLGIAGTAVAILAALWLGPGRIDGFSGMIVHDGMGLFITLTILATALLTLMMATG
                     YSEWEGTQKGEFYALLLFSTAGMLFMAKGTDLMIVFLGLETMSIPIYCLVGFHRNRMT
                     SLEGALKYFLLGAFASGFLLYGIALMYSVTGTTKIPVIAGMIGDLRLFGNPVFVVGVG
                     LLLVGFAFKVSLVPFHMWTPDAYQGAPTVVTAFMAAGVKAAGFAALLRVTLLTFPALG
                     PVMTNILWVLAFLTMTVGNLSALLQDNMKRMLAYSSIAHAGYILVGLVSGDVAGGQAA
                     LFYLLVYAFMNLGAFGVAMLLAHKEDDGYDIGNFAGIGFRYPVLGGLLTLFLVSLAGI
                     PPTAGFIGKFYLFSAAVKNGYVGLAVLGVLNSAVSIYYYLRPVVYMYMLPAPAGAPVP
                     RPPRTAFSLALCVSAAAVLVLGIAPRSVLAFAERSILSLLM"
     gene            complement(1588..>2163)
                     /locus_tag="VF357_00860"
     CDS             complement(1588..>2163)
                     /locus_tag="VF357_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX7522724.1"
                     /translation="LQGIEGAILQMVNHGVSTGALFLLVGIIYERRHTRLISEFGGLS
                     KVMPLFALCFMVVTLSSIGVPGTNGFVGEFLILLGAFKVQKWYAVVAATGVIFAAVYM
                     LWMFQRVMFGKVTNEENLRLTDMNGREVAYMLPLLLFILWIGVYPQPFLRRMDASVNA
                     FVTRFEAKKQAALAVSPAGETMLVRYFDVKK"
BASE COUNT          440 a          697 c          733 g          293 t
ORIGIN      
        1 ccacgcgcct cgggtggcac ggacgacagg tgtgcgtcgc acgcaaaccc cggtgcccgg
       61 agtgtggcct cgctgccgtc tgcccgaagc ggggggtgta accgctacat cagcagggag
      121 agaatggaac gctcggcgaa cgcgaggacg gagcggggcg cgatcccgag gaccaggacc
      181 gccgccgcgg agacgcatag ggcgagggag aaggcggtgc gcggcggccg gggaaccggg
      241 gccccggccg gcgcggggag catgtacatg taaacgaccg gccgcaggta gtagtagatc
      301 gacacggcgc tgttcagcac gccgaggacg gccagcccga cgtagccgtt tttcaccgcc
      361 gcgctgaata ggtagaactt cccgatgaat cccgcggtgg gcgggatccc ggccagcgac
      421 acgagaaaga gcgtgagcag gccgccgagc acggggtagc ggaagccgat ccccgcgaag
      481 ttcccgatgt cgtacccgtc gtcctccttg tgcgcgagga gcatcgccac gccgaaggcg
      541 cccaggttca tgaaggcgta caccagcagg tagaagaggg ccgcctgccc gcccgcgacg
      601 tccccggaga ccagccccac gaggatgtac cccgcgtggg cgatggagga ataggcaagc
      661 atccgcttca tgttgtcctg gagcagggcc gacaggtttc ccaccgtcat ggtgaggaac
      721 gcgaggaccc agaggatgtt cgtcatcacc ggtccgagcg ccgggaaggt cagcagcgtc
      781 acgcgcagga gcgccgcgaa gccggccgcc ttcactccgg cggccatgaa cgccgtcacc
      841 accgtgggcg ccccttggta cgcatcgggg gtccacatgt ggaaggggac gagcgacacc
      901 ttgaaggcga acccgacgag gagcagcccc acccccacca cgaacaccgg gttcccgaag
      961 aggcgcagat cgccgatcat ccccgcgatc accgggattt tcgtggtccc ggtcaccgag
     1021 tacatcaggg cgatcccgta gagaaggaac cccgaggcga acgccccgag gaggaaatat
     1081 ttcagcgccc cctcgagcga cgtcatccgg ttccgatgga acccgaccag gcagtagatg
     1141 gggatcgaca tcgtctccag cccgaggaag acgatcatga gatccgtccc cttcgccatg
     1201 aagagcatcc ccgcggtgga gaagaggagc agggcgtaga actccccttt ctgcgtccct
     1261 tcccattcgg agtagccggt ggccatcatc agggtgagca gcgcggtggc caggatcgtc
     1321 agggtgatga acagccccat cccgtcgtgg acgatcattc cggaaaaccc gtcgatccgc
     1381 ccgggaccca gccacagcgc ggccaggatc gcgaccgccg tgcccgcgat cccgagatgg
     1441 cacaaagact tcttcccgcg ctccggaagg accaggtcgg cgagcaggac cgcgatcgcc
     1501 gtggcgatca cgaccagctc ggggaggatg ctgtagagcc cctgtaccag cgcagtcgga
     1561 tcgaagggaa tcggcgtcat cgcgcgctca cttcttcacg tcgaagtaac ggacgagcat
     1621 cgtctccccc gcgggggaga cggcgagcgc cgcctgtttc ttcgcctcga agcgcgtgac
     1681 gaacgcgttc acggaggcgt ccatccgccg caggaacggc tgcgggtaga ccccgatcca
     1741 aaggatgaac agcagcagcg ggagcatgta cgcgacctcc cgcccgttca tgtcggtgag
     1801 gcgaaggttc tcctcgttgg tcaccttgcc gaacatcacc cgctggaaca tccagagcat
     1861 gtagacggcg gcgaagatga ccccggtcgc ggcgacgacg gcgtaccatt tctggacctt
     1921 gaacgccccc agcaggatca ggaactcgcc gacgaagccg ttcgtgccgg ggacgccgat
     1981 cgaggagagg gtgacgacca tgaagcagag ggcgaagagc ggcatcactt tggacaaccc
     2041 cccgaactcg gagatgaggc gggtgtgacg ccgctcgtag atgatcccga cgaggaggaa
     2101 gagcgccccg gtggagacgc cgtggttgac catctggagg atggcgccct cgatcccctg
     2161 cag
//
LOCUS       DASVJL010000049         4649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_288354, whole genome shotgun sequence.
ACCESSION   DASVJL010000049 DASVJL010000000
VERSION     DASVJL010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288354"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VF357_00865"
     CDS             complement(<1..255)
                     /locus_tag="VF357_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7522725.1"
                     /translation="MKTQVRNYKLYIAGKWVSDKESMPVIDKYTGETFANVPLASRET
                     TERAIFAAHEAFPAWSRTPAHKRYKILSNVSRLLEQRQEEI"
     gene            complement(286..1569)
                     /locus_tag="VF357_00870"
     CDS             complement(286..1569)
                     /locus_tag="VF357_00870"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HEX7522726.1"
                     /translation="MGVELETIIPLSMGKLKFGVGATEELGYELKILGARKVLLVTDK
                     GLWDHGLIDKVRKIIEGEKIALAIFDEVRCEPTDRSMRKAIEAAGGADFDAVVALGGG
                     SALDTAKAMNLYGTHPAPLLEYVNAPIGKGKPVPGPLRPLIALPTTAGTGSETTTVIV
                     MDFLDLHVKTGISHQYIRPTVALVDPLNSLSAPAAVTANTGLDVLTHAAESFTIKPYN
                     MRPKTKDPAARPPYIGSNPISDLWSEKAVEWVGTYLRRAYYNNVDVEAKSHMAMAATF
                     AGIGFGNAGVHIPHALGYPIAGMVKGWVPPGYATKEPMVPHGLSVALTAAANFRFTAP
                     TDPGRHARIARLLGVNTEGLSEVEAARKLPDAFNELMRDIGAPNGLSAIGYTEADIPG
                     LVEGGWMQQRLLVGSPRPVTKADLSNIVEQSMRLW"
     gene            complement(1589..2293)
                     /locus_tag="VF357_00875"
     CDS             complement(1589..2293)
                     /locus_tag="VF357_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7522727.1"
                     /translation="MILEMRDVHTFYGTSHVLFGVSLDVDRGEVVCLMGRNGAGKSTT
                     FRTVMGLTPPRAGTVLFQGKDVTRLKVHTKARLGIGYVPEDRQIFPELTVRENLDIGR
                     SSGIRRKDGWNIDRIYGLFPVLEKYDRKPGGQLSGGEQQMLTIARTLMGNPDLVLLDE
                     PTEGLAPVVVIALKEMILRLKEMGTTILLCEQNVKFAVKVSDRVFIIDNGAIRYRNDI
                     EGFRNDEAVQKKYLAV"
     gene            complement(2290..3057)
                     /locus_tag="VF357_00880"
     CDS             complement(2290..3057)
                     /locus_tag="VF357_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7522728.1"
                     /translation="METRPPILEIRRLAKAFGGLKVTNDVSFDVRKGELSAIIGPNGA
                     GKTTLFNLITGKLVPDGGEVLYKGERIDGLHPADIARRGIGRAFQITSIFKERTTLEN
                     VLVAVLSREGRTTRLMRRALSHNREVKESMGLLSMVALADRANMRAGSLPHGDQKRLD
                     IAVALALNPELVLLDEPMAGMSPEERSVTVELIRRIWKERSLTLVFIEHDMDVVFGIS
                     EWIRVLNQGGMLAEGTPAEISRNRDVITAYLGEEIVE"
     gene            complement(3032..4018)
                     /locus_tag="VF357_00885"
     CDS             complement(3032..4018)
                     /locus_tag="VF357_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7522729.1"
                     /translation="MKGPSTAPGNAARGQLLQWAAVLLFFALVPLGRRSFFITMANEV
                     LIMGLFAMGFNLLYGVTGMLSFGQAAYYGAGGYTVGLLLTKGVLPYSAALPLAPVVGA
                     ALALLLGPLCIRLSGVYFTMLTLAFAELVWGVVFKWYGFTGGDNGIQGIPVPKWFHDP
                     LHYYYFTLAIVVAAVAVLRRIVESPFGAVLQSIRENPERTAFLGIRARRYQLAAMVIS
                     GAFSGLAGGLFAGFHRSIGPDMLHWTKSGEVILMSILGGVSSFFGPLVGAGVILFIED
                     MIGKYTEFWEIWIGGIMLAIVIFFPRGVIGTLYQWTHRKRSGERNGNATAHP"
     gene            complement(4015..>4649)
                     /locus_tag="VF357_00890"
     CDS             complement(4015..>4649)
                     /locus_tag="VF357_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7522730.1"
                     /translation="VFLRRIYGRGGEAGFQILLTYSFILIIDDVVKMIWGTDYKSIER
                     PESLSASFTLGDISVPRYDLVVILVGILVAAGMWWIIRKTRFGRNLRAISANREMASC
                     LGVNVPLTLSLVFGLATALGGLSGALSAPTRTVTPGAGIEVIIGSLIVVVIGGLGNFW
                     GAFVGALIIGEVTAFGILFLPQWAILFSYAVMALVLVFRPEGLLARKGSA"
BASE COUNT          780 a         1608 c         1465 g          796 t
ORIGIN      
        1 gatctcctcc tggcgctgct cgaggagccg ggagacgttc gacaggatct tgtacctctt
       61 gtgggccggc gtgcgcgacc aggccgggaa cgcctcgtgg gcggcaaaga tggcgcgctc
      121 cgtcgtttcc cgcgacgcga ggggcacgtt cgcgaacgtc tcgccggtgt acttgtcgat
      181 gaccggcatc gattccttat ccgacaccca cttcccggcg atgtagagct tgtaattccg
      241 gacctgagtc ttcattggaa aactctcctt ttcacggggg gatatctacc aaagcctcat
      301 ggactgctcg acaatgttgg acaggtccgc tttcgtcacc ggccgcgggc tgcccaccag
      361 aagccgctgt tgcatccagc cgccctccac caggccgggg atgtcggcct ccgtgtatcc
      421 tatcgcggaa aggccgttcg gcgcgccgat gtcccgcatc aactcgttga atgcatcggg
      481 tagtttcctg gccgcctcca cctccgacag tccctccgtg ttcaccccga gcagacgcgc
      541 gatccgggcg tgccggcccg gatcggtcgg ggccgtgaaa cggaagttgg ccgcggcggt
      601 cagcgccacc gacagaccgt gggggaccat gggttccttc gtcgcgtacc cgggggggac
      661 ccatcccttg accataccgg cgatcggata ccccagcgcg tgcgggatgt ggactcccgc
      721 gttcccgaag ccgatccccg cgaaggtcgc cgccatggcc atgtggctct tcgcttccac
      781 gtcgacgttg ttgtaatagg cccgccgcag gtaggttccg acccactcga cggccttctc
      841 gctccagaga tcggagatcg ggttggaccc gatgtagggc gggcgcgcgg cagggtcttt
      901 cgtcttcgga cgcatgttgt acggcttgat ggtgaacgat tcggccgcgt gggtcagcac
      961 gtccagtccc gtgttcgccg tcaccgccgc cggcgccgac agggagttca acgggtccac
     1021 gagcgccacc gtggggcgga tgtactggtg ggagatgccg gtctttacgt gcagatccag
     1081 gaagtccatg acgatgacgg tggtcgtctc gctcccggtt cccgcggtcg tcggaagggc
     1141 gatcaacggc ctcagcggtc cgggaacggg cttccccttg ccgatgggcg cgttgacgta
     1201 ttccagcagg ggcgcggggt gcgttccgta caggttcatc gcctttgccg tgtccagcgc
     1261 gctgcctccg cccagcgcga cgacggcatc gaagtccgcg ccgcccgccg cctcgatcgc
     1321 tttccgcatc gaccggtccg tcggctcgca ccggacctcg tcgaagatgg ccagggcgat
     1381 cttctcgcct tcgatgatct tcctgacctt gtcgatcagc ccgtggtccc acagcccctt
     1441 gtcggtcacc agcaggacct tccgcgcccc caggattttc agctcgtatc cgagctcctc
     1501 cgtcgccccg acgccgaatt tcagtttccc catcgacagc gggatgatcg tttccagttc
     1561 cacacccact gcgcgcctcc tcggccgcct acacggccag gtattttttc tgcacggcct
     1621 cgtcgttccg gaagccttcg atgtcgttcc ggtagcggat cgccccgttg tcgatgatga
     1681 acacccggtc ggagaccttc acggcgaact tcacgttctg ctcgcagagg aggatggtgg
     1741 tccccatctc cttcaggcgg aggatcatct ccttgagggc gatgaccacg accggggcca
     1801 gcccctccgt cggctcgtcc agcaggacca ggtcgggatt ccccatgagg gtccgggcga
     1861 tggtgagcat ctgctgctcg ccccccgaga gctgcccccc gggtttccga tcgtacttct
     1921 ccagcacggg gaaaagcccg tagatcctgt cgatgttcca cccgtccttc cggcggatgc
     1981 ccgaggaacg gccgatatcg aggttctccc gcacggtcaa ctccgggaag atctggcggt
     2041 cctccggtac gtagccgatc cccaaccgcg ccttggtgtg gaccttgagg cgggtgacgt
     2101 ccttcccctg gaagaggacg gttcccgcgc gcgggggggt aagccccatc acggtgcgga
     2161 aggtggtgct cttccccgcg ccgttccttc ccatgaggca gaccacctct ccccggtcga
     2221 cgtccaggga gaccccgaag aggacgtggc tggtcccgta gaaggtgtgg acgtcccgca
     2281 tctccaggat cattcgacga tctcctcgcc gaggtatgcc gtgatgacgt cgcggttgcg
     2341 cgaaatctcc gcaggcgtcc cctccgccag catcccgccc tggttgagca cccggatcca
     2401 ctccgagatc ccgaagacga cgtccatgtc gtgctcgatg aacaccaggg tcagcgacct
     2461 ctccttccag atcctgcgga tgagctccac cgtgacggac cgctcctccg gcgacatgcc
     2521 ggccatcggc tcgtccagaa gcaccagctc cgggttcagc gcaagggcca cggcgatgtc
     2581 gagccgtttc tggtccccgt ggggcagtga cccggcccgc atgttcgcac ggtcggcgag
     2641 cgcgaccatc gacagcagcc ccatcgactc cttcacttcg cggttgtgtg acagggcgcg
     2701 gcgcatcagg cgcgtggtcc ggccctcccg cgagaggacg gcgaccagca cgttttccag
     2761 ggtggtgcgc tccttgaaga tgctggtgat ctggaacgcc ctgccgatcc cacgccgcgc
     2821 gatgtcggca ggatggagcc cgtcgatccg ctcccccttg tacagcactt cccctccgtc
     2881 ggggacgagc tttcccgtga tcaggttgaa cagggtggtc ttccccgccc cgttgggccc
     2941 gatgatggcg ctgagctctc ccttccggac gtcgaagctc acgtcgttgg tgaccttcag
     3001 gcccccgaac gccttggcga ggcggcggat ctcaaggatg ggcggtcgcg tttccattcc
     3061 tctccccgct cctcttccga tgcgtccact gatagagcgt cccgatgacc ccccggggga
     3121 agaagatcac gatcgccagc atgatccccc cgatccagat ctcccagaac tcggtgtact
     3181 tgccgatcat gtcctcgatg aagaggatga ccccggcccc caccagcggg ccgaagaagg
     3241 aggagacccc gccgaggatg ctcatcagga tcacctcgcc cgacttggtc cagtgcagca
     3301 tgtccgggcc gatggagcgg tggaaccccg cgaacagccc acccgccagc cccgagaacg
     3361 ccccggagat caccatggcg gcgagctggt accggcgggc gcggatcccg agaaacgcgg
     3421 tccgctccgg gttctcccgg atcgactgga ggaccgcgcc gaagggggat tccacgatcc
     3481 tccggagaac cgcgacggcc gcgacgacga tcgccagggt gaagtagtag tagtgcagcg
     3541 ggtcgtggaa ccacttcggc acggggatcc cctggatccc gttgtccccc ccggtgaagc
     3601 cgtaccactt gaagacgacg ccccagacca gctccgcgaa cgcgagggtg agcatcgtga
     3661 agtagacgcc ggagagccgg atgcacagcg ggcccaggag cagcgccagc gcggcgccga
     3721 cgaccggggc cagcggaagg gcggcggagt acgggaggac ccccttggtg aggagcaggc
     3781 cgaccgtgta cccgcccgcg ccgtagtacg ccgcctggcc gaaggagagc atcccggtga
     3841 cgccgtagag caggttgaac cccatcgcga acagccccat gatcaggacc tcgttcgcca
     3901 tggtgatgaa gaacgaccgg cgccccagcg gcacgagggc gaagaagagc agcaccgcgg
     3961 cccactggag cagctgaccg cgtgcggcgt ttccgggggc ggtggacgga cccttcacgc
     4021 gctccccttt ctcgccagca gcccctcggg acggaacacg aggacgagcg ccatgacggc
     4081 gtagctgaag aggatggccc actgcggcag gaacaggatg ccgaaggcgg tcacctcccc
     4141 gatgatcagc gccccgacga acgcccccca gaagtttccc aggcccccga tgaccaccac
     4201 gatgagcgag ccgatgatga cctcgatccc cgcgcccggc gtcaccgtcc gcgtcggcgc
     4261 ggagagggcg ccggagagcc cgcccagggc ggtcgccagc ccgaagacga ggctcaacgt
     4321 aagcggcacg ttcaccccga ggcacgacgc catctcccgg ttcgccgaga tcgcccgcag
     4381 gttccgcccg aagcgcgtct tccggatgat ccaccacatc cccgcggcca ccaggatccc
     4441 cacgaggatc acgacaaggt cgtaccgcgg gacggaaata tccccgagag tgaaggaggc
     4501 gctcaaggat tccgggcgct cgatcgattt gtagtccgtc ccccagatca tcttcaccac
     4561 gtcgtcgatg atcaggatga agctgtaggt gagcaggatc tggaaccccg cctcccctcc
     4621 ccggccgtag atccggcgaa ggaacacgt
//
LOCUS       DASVJL010000050         3708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1039254, whole genome shotgun sequence.
ACCESSION   DASVJL010000050 DASVJL010000000
VERSION     DASVJL010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039254"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..1346)
                     /gene="prfA"
                     /locus_tag="VF357_00895"
     CDS             complement(279..1346)
                     /gene="prfA"
                     /locus_tag="VF357_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX7522731.1"
                     /translation="MWDKLESVVARMPELERLLSDPSVTGNPREMQRVGRELADLRPV
                     AAAHARYRKIEQELADNRALQDGETDSAMKAMVREEIDRLTSEKDRLAEEIRALLVPK
                     DPNDEKNILIEIRAGAGGDEASAFAGELFRAYGMYADSKRWKVEILSRSETGLGGTKE
                     VIAMIEGRGAYSRLKYESGVHRVQRVPATEAGGRIHTSTVTVAVMPEAEEVDVQIQPD
                     DLRIDVFRSSGPGGQSVNTTDSAVRITHIPTGLVVQCQDEKSQLKNKSKALKILRSRL
                     YDAEMAKRQSERADLRRSQVGTGDRSERIRTYNFPQNRVTDHRIGLTMHQLSAVLDGG
                     FDSFIDALTSQAQVEALRKTG"
     gene            complement(1336..2283)
                     /locus_tag="VF357_00900"
     CDS             complement(1336..2283)
                     /locus_tag="VF357_00900"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX7522732.1"
                     /translation="MPREAKELVLGGQAVIEGVMMKGPLLYAVAVRKANGEIRVRDFP
                     LVESAARKRIAKIPLVRGVVTMAAMLAIGYRALQYSADEAMEDAEGASAAAKDTGAGA
                     KSGGLAMAGAMAMALLLGVGLFFLFPLYATHLLAGLVPALRGSIAFNLADGAIRVLIF
                     VLYIVGITRMKDIRRVFEYHGAEHKVINAYEANADLTPDAVRVYPRLHPRCGTSFLLF
                     VMVISILVFSLIPRESSLLAKALLRLPLIPAIAGISYEVLRLSAKKTRSPWFRALVAP
                     GLWLQRLTTLEPDLPQIEVAIASFRRVSGPPGSEVSLVG"
     gene            complement(2283..2984)
                     /gene="thyX"
                     /locus_tag="VF357_00905"
     CDS             complement(2283..2984)
                     /gene="thyX"
                     /locus_tag="VF357_00905"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX7522733.1"
                     /translation="MNVSLLTCTPDPERVVALAARLCYSPASIGELREEISRRDAMNL
                     VRRVLSMGHASVLEHVTLTYGVEGISRAASHQLVRHRIASYSQQSQRYVAAKFGYVTP
                     PTVAASPGLLAAYERHMAACSRLYAKMMKAGVPPEDARFALPNATETKILITMNAREL
                     HHFFALRTCRRAQWEIREMAKRMLSAARERAPLLFETAGPGCVRGRCPEGKMSCGKPA
                     EVRREIAALGKRVGA"
     gene            complement(3010..3219)
                     /gene="rpmE"
                     /locus_tag="VF357_00910"
     CDS             complement(3010..3219)
                     /gene="rpmE"
                     /locus_tag="VF357_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX7522734.1"
                     /translation="MKENMHPKYVTATVKCACGNTFETKSVTEEIKVAICSNCHPFFT
                     GKQKLIDTAGRIEKFEKKYGKGAKA"
     gene            complement(3243..>3708)
                     /gene="rho"
                     /locus_tag="VF357_00915"
     CDS             complement(3243..>3708)
                     /gene="rho"
                     /locus_tag="VF357_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX7522735.1"
                     /translation="DSITRLARAYNAVVPPSGKVLSGGVDANALQKPKRFFGAARNIE
                     EGGSLTIIATALIETGSRMDEVIFEEFKGTGNNELVLDRKIAEKRIFPAIDINKSGTR
                     KEELLLEKNLLQRVWILRKFLSPLSPAESMEFLLDKISKTKTNKEFIESMNA"
BASE COUNT          574 a         1272 c         1207 g          655 t
ORIGIN      
        1 atcccgctcc gatgcgccac cgtgttcagg gacgcagggc gcagcggggg gcttcctact
       61 tcgcggcgtc tggagcgtag cggagacagg gacgtcggga gcaagcggag gcgcaggcga
      121 ggagaccatg gacggtcgac cgctcctgcg catccatccg cccgcggcac ttggccatcc
      181 ctggccatcg acgatggtag cggagcggaa gcccccccgc gagcccggag tcctgccccc
      241 cgttggtcac atgcaaccgt atttacgaac tggagcactt agccggtctt gcggagcgcc
      301 tcgacctggg cctgggacgt cagggcgtcg atgaaggagt cgaacccgcc gtcgagcacg
      361 gcggagagct ggtgcatcgt cagcccgatc cggtggtcgg tcacgcgatt ctgggggaaa
      421 ttgtaggtgc gaatccgttc gctgcggtcc ccggttccca cctgggagcg gcgcaggtcg
      481 gcccgctcgg actggcgttt cgccatctcg gcgtcgtaga ggcgcgagcg gaggatcttc
      541 aaggccttcg acttgttctt caactgggac ttctcgtcct ggcactggac gacgagcccc
      601 gtgggaatgt gcgtgatgcg taccgcggag tcggtggtgt tgacgctctg gcccccggga
      661 ccggaggagc ggaacacgtc gatccggagg tcgtccggct ggatctggac gtccacctct
      721 tccgcctcgg gcatcaccgc cacggtcacc gtggaggtgt ggatccgtcc ccccgcctcc
      781 gtcgccggca cacgctggac ccggtggacg ccgctttcgt acttcaggcg gctgtacgcc
      841 ccgcgtccct cgatcatggc gatcacttcc ttggtccccc ccagccccgt ctcgctgcgg
      901 gagaggatct cgaccttcca gcgcttggag tcggcgtaca tcccgtaggc gcggaagagt
      961 tctccggcga aggcggacgc ctcgtctccc ccggcgccgg cgcggatctc gatgaggatg
     1021 ttcttttcgt cgttggggtc cttggggacg agcagggcac ggatctcctc cgcgagacga
     1081 tccttttccg aggtcagccg atcgatctcc tcgcggacca tcgccttcat cgcggaatcg
     1141 gtctcgccgt cctggagggc ccggttgtcg gcgagctcct gctcgatctt ccggtaccgc
     1201 gcgtgggccg ccgccaccgg gcgcagatcg gcgagctccc gcccgaccct ctgcatctcc
     1261 cgcgggttcc ccgtgaccga agggtccgac agcagccgct ccagctccgg catccgcgcc
     1321 acgaccgatt cgagcttatc ccacaaggga aacctccgaa cccggcggac ccgacacgcg
     1381 ccggaacgac gcgatcgcca cctcgatctg gggcaagtcc ggctccaggg tggtcagccg
     1441 ttgcagccac agccccggcg cgacgagcgc ccggaaccag ggggaccggg tctttttcgc
     1501 cgacagccgc agcacctcgt aggaaatccc cgcgatcgcg gggatcagcg ggagccgcag
     1561 gagcgccttc gcgagcaggg agctctccct cgggatgagg gagaagacga ggatgctgat
     1621 caccatgacg aacaggagga agctggtgcc gcaccgcggg tgcaggcgcg ggtacacccg
     1681 caccgcatcg ggcgtaaggt ccgcattcgc ctcgtaggcg ttgatcacct tgtgctccgc
     1741 gccgtggtat tcgaagaccc tccgtatgtc cttcatccgc gtgatcccga cgatgtacag
     1801 gacgaagatc agcacccgga tcgccccgtc cgcgaggttg aaggcgatcg acccccggag
     1861 ggcggggacc aggccggcaa ggagatgggt cgcgtacagc ggaaacagga aaaagaggcc
     1921 gacccccagg agaagcgcca tcgccatcgc gcccgccatg gccaatcccc ccgacttcgc
     1981 gcctgccccc gtgtccttcg cggcggccga agcgccttcc gcgtcctcca tcgcctcgtc
     2041 cgccgagtat tgcagggcgc ggtatccgat cgccagcatc gccgccatcg tcaccacccc
     2101 gcgcaccagg gggattttcg cgatccgctt ccgcgccgcc gattccacga gggggaagtc
     2161 ccggacccgg atctccccgt tggccttccg caccgcgacc gcgtatagca gcggcccctt
     2221 catcatcacg ccctcgatga ccgcctggcc gccgaggacg agctccttcg cttcccgggg
     2281 catcaagcgc cgaccctctt cccgagagcc gcgatttccc ggcgcacctc cgccggcttg
     2341 ccgcagctca tcttcccttc cgggcaccgg ccccggacgc agccgggacc cgccgtctcg
     2401 aacagcagcg gcgcccgttc cctcgcggcc gagagcatcc gcttcgccat ctcgcggatc
     2461 tcccactgcg cccgtcggca cgtgcgcagg gcgaagaaat ggtgcaattc acgcgcgttc
     2521 atcgtgatca ggatcttcgt ttccgtcgcg ttgggaaggg cgaagcgcgc gtcctccggc
     2581 gggacgccgg ccttcatcat tttcgcgtag agccgggaac aggccgccat gtggcgctca
     2641 tacgcggcca aaagaccggg ggaggccgcg acggtcgggg gagtgacgta tccgaacttc
     2701 gcggcgacgt accgctggct ctgctgggaa tacgaggcga tacggtggcg gaccagctgg
     2761 tgcgaggcgg cgcgggagat cccctccacg ccgtaggtga gggtcacgtg ctcgaggacg
     2821 gaggcgtgcc ccatcgagag gacccgtcgg acgagattca tcgcgtcccg ccgggagatc
     2881 tcctcccgga gttcaccgat cgacgcggga gagtagcaca gcctcgccgc gagcgcgacg
     2941 acgcgctccg ggtcgggggt gcaagtgagc agcgagacgt tcacggccgg aaggcgcccc
     3001 ccgggggtac taagccttcg cccccttgcc gtacttcttc tcgaacttct cgatccgtcc
     3061 cgcggtgtcg atcagcttct gcttgcccgt gaagaacggg tggcagttgg agcagatggc
     3121 caccttgatc tcttccgtga ccgacttcgt ctcaaaggtg ttcccgcagg cgcacttcac
     3181 cgtcgcggtg acgtacttcg ggtgcatgtt ttccttcatc gttccgtttc ctttccgccg
     3241 tttcaggcgt tcatcgattc gatgaactcc ttgttcgtct tggtcttgga gatcttgtcc
     3301 aggaggaact ccatgctctc cgccggcgac aagggggaga ggaacttccg caggatccag
     3361 acgcgctgga ggaggttctt ctcgagaaga agctcctctt tccgcgtgcc ggacttgttg
     3421 atgtcgatgg ccgggaagat ccgcttctct gcgatcttcc ggtccaggac gagttcgttg
     3481 ttgccggttc ccttgaactc ctcgaagatg acctcgtcca tccggctgcc ggtctcgatg
     3541 agagccgtcg cgatgatggt cagcgaaccc ccctcctcga tgttccgggc cgccccgaag
     3601 aaccgcttcg gcttctggag ggcgttggcg tcgacgcctc cggagaggac cttccccgac
     3661 ggggggacga cggcgttgta ggcgcgcgcc agccgcgtga tgctgtcg
//
LOCUS       DASVJL010000051         2334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_663948, whole genome shotgun sequence.
ACCESSION   DASVJL010000051 DASVJL010000000
VERSION     DASVJL010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663948"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..895
                     /locus_tag="VF357_00920"
     CDS             179..895
                     /locus_tag="VF357_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7522736.1"
                     /translation="MLLTVDHLSVFYGAIQALREVSFTVAKGEIVSLIGANGAGKSTA
                     LRALSGVVRTEAGSIVHDGKSIGGLPSHRIARLGIAHVPEGRGVFANMTVRENLEMGA
                     YTRSSRKEVEESFERVFRLFPRLSERAAQLAGTLSGGEQQMLAIGRGLVQRPDLLLLD
                     EPSMGLSPLLVNEIFRMIEEINKAGTTILLVEQNASMALSIADRAYVLETGEIVLEGN
                     ASDLLEDPKVRAAYLGDDSG"
     gene            984..>2334
                     /locus_tag="VF357_00925"
     CDS             984..>2334
                     /locus_tag="VF357_00925"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7522737.1"
                     /translation="MPYPLTLATMLQRNRLLFGKKEVVGRDFSGIHRYTYADYNIRVA
                     KLANALKRLGVRPGERVATFGWNSHRHLEAYFAVPCMGAVLHTLNIRLFEDQFTFIVN
                     HAEDAVMLVDEDLLPAVEALADKFPTVRAYVIMSDKKIVPPTKLSPVYSYEALLDAEA
                     AEYDWPSLPDENSRAALCYTTATTGKPKGVPYSHRGIYLHALAICAADVLGIREADTV
                     MPVVPMFHANAWGIPFAAAWMGTKQVFPGSRPDAKALLSLIQEEKVTITAGVPTVWMG
                     VLQLMEQEKYDVSSLKAIVCGGSAAPKSLIEKVEKGFGVPFVHAYGLTETYPVVLVSR
                     PKSYMKDLPGDELYAYKGKQGTLVPGLEMRVVTEDGSEVPRDGKSMGELRLRGPWITE
                     EYYRDPERTRETVRDGWLCTGDVVTVDAEGYVQIQDRTRDLIKSGGEWISSIDLENTI
                     "
BASE COUNT          439 a          696 c          787 g          412 t
ORIGIN      
        1 tcgagcacca gatgcgcctg gtgatgggga tctgcgagcg gctgatcgtg atggacttcg
       61 gccaggtgat cgcccgaggt cttcccgggg agatccggcg gaaccatcag gtgatcgagg
      121 cgtacctcgg gaagagggac gacacggacg ggggaggtat caggaagaag gcgagataat
      181 gctactaacc gtcgatcatt taagcgtatt ttacggagcc atccaggcgc tccgggaggt
      241 ctcgttcacc gtagcgaaag gggagatcgt ctccctgatc ggtgcgaacg gggctgggaa
      301 gagcaccgcc ttgcgggcac tttccggcgt cgtccgtacc gaagcgggga gcatcgtaca
      361 cgacggaaaa tcgatcgggg gactcccatc ccaccggatc gcgcgcctgg ggatcgccca
      421 cgttccggag ggtcggggcg tcttcgccaa catgaccgtc cgggagaatc tcgaaatggg
      481 ggcgtacacc cgctcttcca ggaaagaggt ggaggagagc ttcgaacggg tcttccgtct
      541 cttcccacgt ctgtcggaac gcgcggccca gctcgccggg accttgtcgg gaggggagca
      601 gcagatgctc gcgatcggac ggggtctcgt ccagcgaccc gatctgctgc ttctcgatga
      661 gccgtccatg gggttgtctc ccctgctggt gaacgagatc ttccggatga tcgaggagat
      721 caacaaggcg gggacgacga tcctcctcgt cgaacagaac gcttccatgg ccctctccat
      781 cgcagaccgc gcctacgtgc tcgagacggg ggagatcgtg ctcgaaggaa acgcgtccga
      841 tctgctggag gatccgaagg tacgggccgc ttacctggga gacgattcgg gatgagtcat
      901 tccgttgttg acatcgagtc cggttctggg cgaaatgaca ttcgcccatc atttttcgcg
      961 ggctatgccc agggaggtgc cgcatgccgt acccgctgac cctcgccacg atgcttcaac
     1021 ggaaccgtct cctattcggc aagaaagagg tcgtcggccg ggatttctcc ggcatccacc
     1081 gttacacata cgccgattac aacattcggg tcgcgaagct cgccaacgcg ctgaagcggc
     1141 tgggcgttcg gccgggcgag cgggtcgcca ccttcgggtg gaacagccac cgtcatctcg
     1201 aggcgtactt cgccgtcccg tgcatgggcg ccgtgctcca caccctgaac atccgtctct
     1261 tcgaggacca gttcaccttc atcgtcaacc acgcggagga tgccgtgatg ctggtcgacg
     1321 aggacctgct cccggccgtc gaggcgctgg cggacaagtt tccgaccgtt cgcgcctacg
     1381 tgatcatgag cgacaagaag atcgtcccgc cgacgaagct ctccccggtc tactcgtacg
     1441 aggcgctgct cgacgccgag gcggcggagt acgactggcc ttcgctgccc gacgaaaatt
     1501 cgagggcggc gctctgctac accaccgcca cgaccggaaa accgaagggg gtcccgtact
     1561 cccaccgggg gatctacctg cacgccctcg cgatctgcgc ggccgacgtc ctggggatcc
     1621 gggaggcgga caccgtgatg ccggtcgtgc cgatgttcca cgccaacgcc tgggggattc
     1681 cattcgccgc ggcgtggatg gggacgaagc aggtgtttcc aggctcccgg cccgacgcga
     1741 aggcgcttct ttcgttgatc caggaggaga aggtgacgat caccgcgggg gtacccaccg
     1801 tctggatggg ggtcctgcaa ctgatggagc aggagaagta cgacgtgtcg agcctgaagg
     1861 cgatcgtctg cggcgggtcc gccgccccga aatccctcat cgaaaaggtg gagaaggggt
     1921 tcggggtccc cttcgtccac gcctacgggt tgacggaaac gtacccggtc gtgctggtct
     1981 cccgtcccaa atcgtatatg aaggatcttc ccggggacga gttgtacgcg tacaagggga
     2041 agcaggggac gctcgtgccg ggtctcgaga tgcgcgtggt taccgaggac ggctccgagg
     2101 tcccgcggga cgggaagtcg atgggggaac tgcgtctccg tgggccttgg atcacggaag
     2161 agtattaccg cgacccggaa cggacgaggg agacggtccg ggacgggtgg ctgtgcaccg
     2221 gtgacgtcgt gaccgtggac gccgaaggat acgtgcagat ccaggaccgg acgcgcgatc
     2281 tgatcaagag cggcggggag tggatctcct ccatcgatct cgagaacacg atca
//
LOCUS       DASVJL010000052         3386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1320204, whole genome shotgun sequence.
ACCESSION   DASVJL010000052 DASVJL010000000
VERSION     DASVJL010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320204"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..2855
                     /locus_tag="VF357_00930"
     CDS             213..2855
                     /locus_tag="VF357_00930"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7522738.1"
                     /translation="MPNRVHWLLGAVLFAMIAGGPTPSAGEEALVEKYTLGNGLTVVI
                     RPNPSSPVVAVQAWVKAGSTTEMEARAGMSHILEHMAFKGTKRRGPGEIAREVEEVGG
                     EINAYTSFDQTVYHITLSGRFLENALDILADALGNSVFDPEELSREREVILEEVRMNE
                     DNPARVVSNALFREAYKVHPYGRPVIGHVDTIRKTTREDLLAYFHANYVPGNMVLVIA
                     GAVDPKTSRPLIERTFGQLPPGSAPEAPRPVEPPQNETRVVVQEKDARRAYLDMGFHG
                     PSMLDPDVYAWDLLSMILGSGETSRLYRGVKDGKGLVDSVSASSYTPRDPGLLYIGGT
                     LSPEKARPALKEILLETFRMVAAPPEGAELARAKTSTEADFLYSLESQSALARHIGFY
                     ETTLNDAAFEQTYLRKIRAVTADDIRTVAKKYLSPGNLTVSAVFPTGQEGILPVEEVR
                     AIARQAFAEATAAAAKAGKKRNVVKEVLSNGIRVIVRENRAVPVVAVQAGFLAGVRGE
                     PKEKGGVTNLTAGMLVKGTMRHTAREIAEAVENMGADLSGYSGRNSFGLQGKFLQRDF
                     EKGFRLFAESLLEPTFPAEELEKKRIETMGALKQQKDQLTQATFLLFLGAQYGDHPYG
                     RNPLGTENAVLAITPEDLKAYYERWTDPRNMVIAISGDIDAGEAVAAVRKAFGGMPQR
                     PGFAALGALPVPSHDAVMKVEERRDKQQAHFVIGYPGARFTDPDRYALDVLGSALAGM
                     GGRLFVNLRDKKSLAYSVTSFSSEQVDPGFFAFYMGTSADKLDGAIADTLAEIADVKK
                     GGVTREEFERAKKWMIGTYEIGLQSNGSYADKMVYNELYGTGYEETFAAPEKIAAVSF
                     SDVNRLAASVLDPEKYTIAILRGK"
BASE COUNT          620 a         1061 c         1155 g          550 t
ORIGIN      
        1 cacctgccac tccgtgatcg aggacatcct catcttcatc gtcatcgggg ggaacggcct
       61 cgcgatcctc gtcgttcggc tgggcctggc ggtcttgctg accggcctca tggcccgggt
      121 ctggaagccg gcgtgaaccg cttcaccctt cgggatcatc cattgaagaa ggtttcgaag
      181 cgcaggaaac cgccggggga cgaagaatga gcatgccgaa tcgggtgcac tggctgctgg
      241 gcgcggtact gttcgccatg atcgcggggg gaccgactcc ctccgccggg gaggaagcgt
      301 tggtcgaaaa atacacgctc ggaaacgggc tgacggtcgt gatccggccg aatccttcgt
      361 cgccggtcgt ggcggtgcag gcgtgggtca aggcgggaag caccacggaa atggaggccc
      421 gcgcggggat gtcccatatc ctcgagcaca tggcgttcaa ggggacgaaa cgcagggggc
      481 ccggggagat cgccagggag gtcgaggagg tggggggcga gatcaacgcc tacacgagct
      541 tcgaccagac ggtctaccac atcaccctct cggggcggtt cctcgagaac gcgctcgaca
      601 tcctcgccga cgcgctcggg aactcggtct tcgaccccga ggagctttcg cgggaacgcg
      661 aggtgatcct cgaggaggtg cggatgaacg aggacaaccc ggcccgggtc gtctccaacg
      721 cgctcttccg ggaggcgtac aaggtccacc cgtacggacg ccccgtcatc gggcatgtcg
      781 acaccatccg gaagaccacg cgcgaggatc tgctggccta cttccacgcg aactacgttc
      841 ccgggaacat ggtgctggtg atcgcagggg cggtggaccc gaaaacgtcc cggccgctga
      901 tcgagaggac gttcgggcag ctgccccccg gctccgcgcc ggaagccccg aggccggtcg
      961 agccgccgca gaacgagacc cgggtcgtcg tccaggagaa ggacgcgcgg cgcgcctatc
     1021 tcgatatggg gttccacggc ccctcgatgt tggacccgga cgtctacgcc tgggacctgc
     1081 tgtcgatgat cctcggcagc ggggagacgt cgcggctcta ccgaggcgtc aaggacggga
     1141 aggggctggt cgactcggtg agcgcctcct cgtacacccc gagggacccg gggcttctgt
     1201 acatcggagg aaccttgtcg cccgagaagg cgcggccggc cctgaaggag atcctcctcg
     1261 agacgttccg catggttgcc gcccctccgg aaggtgcgga gttggcgcgc gccaagacct
     1321 ccacggaggc cgacttcctc tactccttag agtcgcaatc cgcgctggcc cgccacatcg
     1381 gcttttacga gaccacgttg aacgacgcgg cgttcgagca gacgtacctg cgcaagatcc
     1441 gcgcggtgac cgccgacgac atccggacgg tcgcgaagaa gtacctttcg cccgggaacc
     1501 tgaccgtttc ggccgtcttc cccacggggc aggaggggat tcttcccgtg gaggaggtcc
     1561 gtgccatcgc gcgacaggcg ttcgccgagg cgaccgcggc ggccgcgaag gccgggaaaa
     1621 agcggaacgt ggtgaaggag gtcctctcca acgggatccg ggtgatcgtt cgggagaacc
     1681 gtgccgtccc ggtggtggcg gtgcaggccg ggttcctcgc gggggtgcgg ggcgagccga
     1741 aggaaaaagg cggcgtcacc aacctcaccg cgggaatgct cgtcaagggg acgatgcggc
     1801 acacggcccg ggagatcgcc gaggcggtgg agaacatggg ggcggacctc agcggctact
     1861 cgggccgcaa ctcgttcggg ctgcagggaa agttcctcca gcgcgacttc gaaaaagggt
     1921 ttcgcctgtt cgccgagtcc ctcctcgagc cgaccttccc cgcggaagag ctcgagaaga
     1981 agcgaatcga gacgatgggg gccttgaagc agcaaaagga ccagctgacg caggcgacct
     2041 tcctcctgtt tctcggagcg cagtacggcg accacccgta tggccggaat cccctcggca
     2101 cggagaacgc ggtcctcgca atcacgcccg aggatctgaa agcgtattac gagcggtgga
     2161 cggacccgcg gaacatggtg atcgccatct ccggagacat cgacgccggg gaagcggtcg
     2221 cggcggtccg caaggcgttc ggggggatgc cgcagcggcc ggggttcgcg gcgctggggg
     2281 cgttgccggt cccctcgcac gatgcggtga tgaaggtgga ggagcggcgc gacaagcagc
     2341 aggcgcattt cgtcatcggc taccccgggg cgcggttcac cgatccggac cgatacgcgc
     2401 tcgacgttct gggctcggcg ctcgccggga tgggaggccg gctcttcgtg aacctgaggg
     2461 acaagaagtc gctggcctac tcggttacat cgttttcctc ggagcaggtg gacccgggct
     2521 tcttcgcgtt ctacatggga acgagcgccg acaagctgga cggcgcgatc gccgataccc
     2581 tcgccgagat cgccgacgtg aagaaggggg gcgtgacgcg ggaggagttc gagcgggcga
     2641 agaagtggat gatcggcacc tacgagatcg gcctccagag caacggctcc tacgccgaca
     2701 agatggtcta caacgagctg tacgggacgg gatacgagga gacgttcgcg gctccggaaa
     2761 agatcgcggc ggtcagcttt tcggacgtga accgcctcgc cgcctccgtc ctcgaccccg
     2821 agaagtacac catcgcgatc ctgcggggca aataagcttc caccccggaa ggatccttcc
     2881 ggagatcgat ccatggatgc ggatcaatgc atgacaatct tgccctgcgg cgagtatcat
     2941 cttccctgaa ggttcaggcc gggcatgcgt accgtgcttg atgcgtggac atgatcaccc
     3001 ggtactcctc caaaaacccg cggcggcacg caatgtgcta tttcacgatg aaccaagagg
     3061 atgttgcaac gcccgcaaca tatcgttgcg caagggatag ggtgtcctcc gcaaactaac
     3121 tgctgcgttt cacaacactt aaggaggcga ggatggccca acccgcccgg ttttcttccc
     3181 tttccgaccc ccctgtccgg ggcggcgctg ctcctcctga cgtctctttt ctcgctcctg
     3241 cctgccgcgt cggcgatgga gtcggtcgac ttccgcgaag cggtgaacag ggcgctttcg
     3301 aacaacgcct ttgtctcagc ggcgggagag gatgccgtcg ccgcacgccg cgatgcggac
     3361 gcggcccgcg ggtaccttct cccttc
//
LOCUS       DASVJL010000053         2545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_195122, whole genome shotgun sequence.
ACCESSION   DASVJL010000053 DASVJL010000000
VERSION     DASVJL010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195122"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VF357_00935"
     CDS             complement(<1..748)
                     /locus_tag="VF357_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX7522739.1"
                     /translation="MNMTLTEKILARHAGRKRVAPGELILAKVDLALGNDITSPIAIQ
                     AFRSLGAAAVFDRERIALVPDHFVPNKDIKSAEQVKMMREFAKEFGVTNFFEVGRMGI
                     EHVLLPDEGLVVPGDLVIGADSHTCTYGALCAFSTGVGSTDLAAVMFSGEVWLKVPET
                     MRIVLSGRPAKWVEGKDVILHIIGEIGVDGALYRAMEYAGGGLAHLSMAWRFTMANMA
                     IEAGAKNGIFPFDAATKAYADGRAKRKYEVV"
     gene            complement(729..1514)
                     /gene="pssA"
                     /locus_tag="VF357_00940"
     CDS             complement(729..1514)
                     /gene="pssA"
                     /locus_tag="VF357_00940"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX7522740.1"
                     /translation="MSRGEWRGGDGLRRGVYVLPNLITSGSLFAGFYSIASTYNGNFE
                     KAAIGIVVGAILDGLDGRVARMTHTTTRFGVEYDSLADVISFGVAPAFLAYGWALSAF
                     GRWGWLAAFLYLICGALRLARYNVQITTAEKGKFNGLPIPAAGVFVASMILLYYDLGG
                     SGSFKHFAVLLTIYVLAFLMVSTIKFNAFKDLEPLRRRPFNTLVVFVFLALLVAAEPQ
                     VMIFVFCASYIVSGPVGEVIDAIRRRRTKHTESEKPVHEHDAH"
     gene            complement(1511..2164)
                     /locus_tag="VF357_00945"
     CDS             complement(1511..2164)
                     /locus_tag="VF357_00945"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEX7522741.1"
                     /translation="MSEKKGPMLAPEGWPFVLGGAAFAVVVYLLWPRGIPLAALGLLL
                     SLFSLWFFRNPDRTPPSGTGVVVSPADGRIVYAGESPPGRYALVAGKRVSVFMSPVDV
                     HVNRAPVTGRVASVRYRKGTFHVASVDKASLMNEQVGVAVVTPEGRTVTYVQIAGMLA
                     RRIVCDLKEGDAVRQGQRVGMIRFGSRVDLYLPAEVRLSVAPGDRVRAGESVIGVFP"
     gene            complement(2161..>2545)
                     /locus_tag="VF357_00950"
     CDS             complement(2161..>2545)
                     /locus_tag="VF357_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522742.1"
                     /translation="TLVEAGYSPEMAYFECVHELKLIVDLIYEGGISTMRYSISNTAQ
                     YGDLTRGPRVVSPETKAEMKRILEEIQSGAFAKEWMLENQANRPVFNALTRRGEEHPI
                     EEVGRRLRALMPWMSKGRLVDKTKN"
BASE COUNT          451 a          915 c          774 g          405 t
ORIGIN      
        1 tcacgacctc gtacttccgc ttcgcccttc cgtcggcgta cgccttcgtc gccgcgtcga
       61 acgggaagat cccgtttttc gcccccgcct cgatcgccat gttcgccatg gtgaagcgcc
      121 acgccatcga caggtgcgcg agcccgcccc ccgcgtattc catcgcccgg tagagggccc
      181 cgtccacccc gatctcgccg atgatatgca ggatcacgtc cttcccctcg acccacttcg
      241 cggggcggcc cgaaaggacg atccgcatcg tctcggggac cttgagccac acctcccccg
      301 agaacatcac cgcggcgagg tcggtgctcc ccaccccggt ggagaaggcg cacagggcgc
      361 cataggtgca cgtgtggctg tccgcgccga tcacgaggtc gccgggaacg accaacccct
      421 cgtccgggag caggacgtgc tcgatcccca tccggccgac ctcgaagaag ttcgtgacgc
      481 cgaactcctt cgcgaactcc cgcatcatct tgacctgttc ggcgctcttt atgtccttgt
      541 tcggcacgaa gtggtcgggt accagggcga tccgctcgcg gtcgaacacc gccgcggccc
      601 ccagcgaccg gaacgcctgg atcgcgatcg gactggtgat gtcgttgccg agggcaaggt
      661 cgaccttcgc caggatcagc tctcccggcg cgaccctctt cctcccggcg tgcctggcga
      721 ggattttttc agtgagcgtc atgttcatgg accggcttct cgctttccgt gtgcttggtc
      781 cggcggcgcc ggatcgcgtc gatcacctcg cccaccggtc cggagacgat gtacgaggcg
      841 cagaagacga agatcatcac ctgcggctcg gcggccacca gcagcgccag gaagacgaac
      901 accaccagcg tgttgaacgg gcgccgccgg agcggttcga ggtccttgaa ggcgttgaac
      961 ttgatcgtgc tcaccatgag gaaggcgagc acgtagatcg tcagcagcac ggcaaagtgt
     1021 ttgaaactcc ccgacccgcc aaggtcgtag tagagcagga tcatggaggc gacgaagacc
     1081 cccgccgccg gaatgggcag accgttgaac ttccccttct ccgccgtggt gatctgcacg
     1141 ttgtagcggg ccagacgcag cgccccgcag atgaggtaga ggaacgccgc cagccatccc
     1201 catcgaccga acgcggagag agcccagccg tacgcgagga acgccggagc gacgccgaag
     1261 gagatcacgt ccgccaagga gtcgtactcg acgccgaatc tcgtggtcgt gtgcgtcatc
     1321 cgggcgacgc ggccgtcgag gccgtcgagg atcgcgccca cgacgatccc gatcgccgcc
     1381 ttctcgaagt tgccgttgta cgtcgatgcg atcgagtaga acccggcgaa cagggaaccc
     1441 gacgtgatga ggttcggcag gacgtacacc ccccgccgca ggccgtcccc cccgcgccac
     1501 tccccgcggc tcacggaaag actccgatca cgctctcgcc ggcgcgtacc cggtctccgg
     1561 gtgcgaccga cagccgcacc tccgccggga ggtacaggtc gacccgggag ccgaaacgga
     1621 tcatccccac ccgctgtccc tggcgcaccg catccccctc cttgaggtcg cacacgatcc
     1681 ggcgggcgag catccccgca atctggacat acgtcaccgt gcgcccctcc ggcgtcacga
     1741 ccgctacccc gacctgttcg ttcatgagcg aggccttgtc gacgctcgcg acgtggaacg
     1801 tccccttgcg gtaccggacc gacgccaccc gccccgtcac cggcgcccgg ttgacgtgga
     1861 cgtcgacggg ggacatgaag acgctgaccc tcttgccggc caccaaagca taccgccccg
     1921 gaggggactc ccccgcatag acgattctac catcggccgg agaaacaacg acccccgtgc
     1981 cggaaggcgg cgtccggtcg gggttgcgga agaaccagag cgaaaacagg gacagaagaa
     2041 gcccgagcgc ggcaagaggg atccctctcg gccaaaggag gtacacgacg accgcgaacg
     2101 ccgctccccc gaggacgaag ggccacccct ccggcgcgag catcggaccc ttcttttcgc
     2161 tcaattcttc gtcttgtcga ccaggcgccc cttcgacatc cacggcatca acgcccgaag
     2221 cctgcggccg acctcctcga tcggatgctc ctcgccacgg cgcgtgaggg cgttgaagac
     2281 cgggcggttg gcctggttct ccagcatcca ctccttcgcg aaggcaccgc tctggatctc
     2341 ctcgaggatc cgcttcatct ccgccttcgt ctcgggagag acgacgcggg ggccgcgcgt
     2401 caggtcgccg tactgcgccg tgttgctgat cgagtaccgc atggtggaga tcccgccctc
     2461 gtagatgagg tcgacgatca gcttgagctc gtgcacgcac tcgaagtacg ccatctccgg
     2521 ggagtacccc gcctcgacca gcgtt
//
LOCUS       DASVJL010000054         3038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1320499, whole genome shotgun sequence.
ACCESSION   DASVJL010000054 DASVJL010000000
VERSION     DASVJL010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320499"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VF357_00955"
     CDS             <1..658
                     /locus_tag="VF357_00955"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX7522743.1"
                     /translation="GIRIRIEAFPPLLCVALTFLWMIVMTNGFNLIDVMDGLAAGVAT
                     VSALALAVVFFLQGERMGLILCLSLVGALLGFLRYNRPPAQIYLGDTGSLLIGFLLGG
                     LAIEGDYTARNPYGWFTALAVFAVPLFEIAFVSWLRSRRGASIFSGSRDHFSLRLRRW
                     KLTRGQTVSASCGAAAVAAVVGLLGMGLAPLPSLFAYGSIGLLFLLVAVWLKSIDMGL
                     "
     gene            669..1988
                     /locus_tag="VF357_00960"
     CDS             669..1988
                     /locus_tag="VF357_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7522744.1"
                     /translation="MILVLGGGITGLAAARTLAAAGEDFLVLEAASEPGGWCRSIRSD
                     RYVFDRSGHFLHLSDPRMREWVLDLPGVLWETIARDARVFLRGKITPFPFQANLYGHA
                     PNLIRRCLTDFAVERIRETVSGPVEEKNFEEWLLRRFGRAMCREFFFPYNRKMWRTSL
                     RGMGYGWTSWSVPVPEIADLLAGARGETRTGMGYNPSFLYPRSGGIGALVSALSTGVR
                     DRVRTATRITRLDLRKKVAWSAGEEAFHFDAVLSTIPLPEMARSCVNLPSGVRAAASY
                     LRWVKVLCVNLGVRDPATTHGHWVYVPERTYPFFRAGFLSNVSAAAAPEGCASVFVET
                     SFPSGARVNVREEVRKATGGLRRMGVLPRGRKPEIIEPVLLDPAYVLFDHARDAAVRR
                     VRAHLREKGVFTAGRYGAWDYYGMEASMADGIRAARDAIQRVRGMSA"
     gene            1985..>3038
                     /locus_tag="VF357_00965"
     CDS             1985..>3038
                     /locus_tag="VF357_00965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7522745.1"
                     /translation="MSGTANRGRTSRARVVHVITMLEWGGAQENTLYSVEHLDPGRYD
                     RVLVAGKGGMLDGRARRIPDLRVVLLDDLVREVRPARDAKAFLSLRSILREEKRVAGG
                     DPLVVHTHSSKAGILGRAAARAAGADAVVHSIHGFGFHDGQSKPTHALYVGLERMASR
                     WTDAFVAVSEENIRAGVQEGIFRRARCRLIRSGFDTARFLAGSRPVGREILGIPDGVP
                     VVGTVAVFKPQKAPLDFVETAWRVASEIPGSRFVMVGDGELRGAAERAVALAGMSDRF
                     SFLGWRPEVPDLMAAFDVFLLTSRWEGLPKVVPQALIAGCPVVATAVDGTREVVDSGV
                     DGVLVRPGDVDALARGV"
BASE COUNT          411 a          951 c         1087 g          589 t
ORIGIN      
        1 cggcatccgg atccgcatcg aagcgtttcc gccgctcctg tgcgtcgccc tcaccttcct
       61 gtggatgatc gtgatgacga acggcttcaa cctgatcgac gtgatggacg gccttgccgc
      121 gggagtggcg acggtctccg ccctggcgct ggccgttgta tttttcctcc agggggaacg
      181 gatgggactc atcctctgcc tctccctggt cggcgccctc ctcggcttcc tccggtacaa
      241 ccggcccccg gcgcagatct atctcgggga caccggctcg ctcctgatcg gtttcctcct
      301 cggggggctc gccatcgagg gggattacac ggcgaggaac ccgtacggtt ggttcactgc
      361 ccttgccgtc ttcgccgtcc ccttgttcga gatcgccttc gtctcctggc tgcgatctcg
      421 ccgtggcgcc tccatctttt ccggcagccg ggatcacttc agcctccggc tccggcgttg
      481 gaagttgacc cgcgggcaga cggtttccgc cagctgcggg gccgccgccg tcgccgcggt
      541 ggtggggctg ctgggaatgg gattggcccc tctcccgtcc ctgttcgcct acggatccat
      601 cgggctcctg tttctcctgg ttgcggtatg gttgaaatcg atcgatatgg gactataaag
      661 gtgcggggat gattctcgtc ctcgggggag ggatcaccgg gctggcagcg gcgagaaccc
      721 tggcggccgc cggggaggat ttcctggtcc tcgaggcggc gtccgagccc ggcggatggt
      781 gccgttcgat ccggtccgac cggtacgtgt tcgaccggag cggtcacttc ctccaccttt
      841 ccgacccgcg catgcgggag tgggtcctcg atctcccggg tgttttgtgg gagacgatcg
      901 cgcgcgatgc gcgagtattt ctccggggga agatcacccc gttccccttt caagccaact
      961 tgtacgggca tgcaccgaac ctcatccggc ggtgcctcac cgatttcgcc gtcgaacgga
     1021 tccgggagac cgttagcggg ccggtggaag agaagaattt cgaggagtgg cttctccgga
     1081 gattcgggag ggcgatgtgc cgggagttct tcttccccta caaccggaag atgtggcgca
     1141 cctcccttcg cgggatgggg tacggctgga cctcctggtc ggtccccgtg ccggagatcg
     1201 cggatctgct cgcaggcgcc cgaggcgaga cgaggacggg gatggggtac aacccgtcct
     1261 tcctctaccc ccgttccggc ggcatcggcg ctctcgtctc cgccctttcg acgggggtcc
     1321 gcgaccgggt gcgcacggcg acccggatta cgcggcttga cctgcggaag aaggtggcct
     1381 ggtctgcggg cgaggaggcg ttccacttcg acgcggtcct ttcgacgatc cccctgccgg
     1441 agatggcccg ctcctgcgtg aacctcccct ccggcgttcg ggcggccgcc tcgtacctgc
     1501 ggtgggtgaa ggtcctctgc gtgaacctgg gtgttcgcga ccccgcgacg acgcacgggc
     1561 attgggtcta cgtccccgaa cggacgtacc cgttcttccg ggccggtttt ctctcgaacg
     1621 tatccgccgc cgcggcgccg gaagggtgcg cgtccgtgtt cgtggagacg agcttcccct
     1681 cgggtgcgcg cgtaaacgtc cgggaagagg tccgaaaggc gaccggggga ctgcggcgga
     1741 tgggagtcct tccacgcggc cggaaacccg agatcattga accggttctt ctcgatcccg
     1801 cgtacgtact attcgatcat gcccgggatg cggcggtgcg acgggtgcga gcgcatcttc
     1861 gcgaaaaggg agtcttcacc gcgggtcggt acggcgcatg ggactattac ggaatggagg
     1921 catcgatggc ggatgggatc cgcgccgccc gcgatgccat ccagagggtc cggggaatgt
     1981 cggcgtgagc gggacggcga atcgcggccg aacgtcccgc gcccgcgtgg tccacgtcat
     2041 cacgatgctc gagtgggggg gcgcccagga gaacaccttg tacagcgtgg aacacctcga
     2101 tcccgggcgg tacgaccgcg ttctggtcgc cgggaaaggc gggatgctcg acgggcgggc
     2161 gcgacggatt cccgatctgc gggtcgtcct gctggatgac cttgtccgcg aggttcgtcc
     2221 ggctcgggac gcgaaggcgt tcctttcatt gcgatcgatc ctccgggagg agaagcgcgt
     2281 ggccggcgga gatcccctgg tcgtgcacac ccacagctcc aaggcgggga tcctcggaag
     2341 ggcggcggcg cgtgcggcgg gagcggacgc cgtcgttcac tccattcacg ggttcgggtt
     2401 ccacgatggg caatcgaagc cgacgcatgc cctctatgtc gggttggaga ggatggcgtc
     2461 ccgctggacg gacgcgttcg tcgcggtgtc ggaggagaac atccgggcgg gcgtgcagga
     2521 ggggatcttc cgtcgtgcgc ggtgccgtct gatccgcagc ggattcgaca cggcaagatt
     2581 cctcgcgggg tcccgcccgg tgggccggga gatcctggga attccggacg gggtcccggt
     2641 ggtgggaacg gttgcggtct tcaagccgca aaaagctccg ctggattttg tggaaacggc
     2701 ctggcgcgtg gcgtcggaga tccctggttc ccggtttgtg atggtcggcg acggggaatt
     2761 gcgcggagcg gcggagcggg ccgtcgcctt ggccgggatg tcggatcgct tttcgttcct
     2821 cggctggcgg cccgaggtcc cggacctgat ggcggccttc gacgtgttcc ttctcacctc
     2881 ccggtgggaa ggtcttccga aggtggtgcc gcaggcgctg atcgcaggat gcccggtggt
     2941 ggcgacggcg gtcgacggga cccgggaggt cgtcgattcc ggggtggatg gtgtgctcgt
     3001 ccgaccgggg gacgtcgatg ccctcgcccg cggcgtcg
//
LOCUS       DASVJL010000055         3361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_7663, whole genome shotgun sequence.
ACCESSION   DASVJL010000055 DASVJL010000000
VERSION     DASVJL010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7663"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VF357_00970"
     CDS             complement(<1..390)
                     /locus_tag="VF357_00970"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX7522746.1"
                     /translation="MRWHRSGTAGFREALERAEVRGSAGAEKVSPVVREILRKVREGG
                     DAALAEYTRRFDRFDPGRKGFAVPPRQVDAAWRRVPAALRRSLELAAERIEAFHRRQV
                     EGGFGVSLSGATLGQRVLPVARAGVYVP"
     gene            complement(403..1704)
                     /gene="murA"
                     /locus_tag="VF357_00975"
     CDS             complement(403..1704)
                     /gene="murA"
                     /locus_tag="VF357_00975"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX7522747.1"
                     /translation="MGKTGTGALTLEIFVIKGGRRLSGTCRVSGAKNAVLPAMAASLL
                     AEGPVEVVGAPRLRDIDTFAKLLGILGAEVTHDDGGADGREVLRIAPGDARKAEAPYE
                     LVKTMRASVLVLGPLLARYGHARISLPGGCAIGARPIDQHLRGLELMGAKTSLSEGYI
                     EASADRLSGTAVSFDMKTVTGTENLMMAASLAEGTTVLSNAALEPEVCDLARLLRAMG
                     ADIEGEGTDEIVVRGVRSLHGARHEVMADRIEAATLLLAGAITGGDVTAQGADASSMN
                     AVVEKLRSSGADVDSVPGGVRVRREGPIRSVNVKTAPYPGFPTDVQAQFMAYMCLGDG
                     FSIISETIFENRFMHVAELQRMGARIDVSGNTAAVKGVPALSGAPLMATDLRASASLV
                     LAGLAASGTTEVLRIYHLDRGYEALEHRLSSLGADIARVKE"
     gene            complement(1677..2558)
                     /gene="prmC"
                     /locus_tag="VF357_00980"
     CDS             complement(1677..2558)
                     /gene="prmC"
                     /locus_tag="VF357_00980"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX7522748.1"
                     /translation="MILERFDPMRLSELLMVCRREMAGVRDAAPGEAEILVSAVTGIP
                     RSRLFLSMDDDVGDSEGRLFPLIARRCGGEPLQYVLGAWDFFGREFLLSPETLIPRPE
                     TEGLAERAVAALRRSPTARPLALDVGTGSGAIAVTLAAEVPAACVVAIDISPGALRKV
                     RENAARHGVASRVRPICCDLLSALKCGGRFVVVVSNPPYVAEGEWPLLPPEVRDHEPP
                     GALLAGRDGLSVLRPLVAGAAGLLSPGGELWCEIGASQGEAVSSLPCGSLRPLGVFPD
                     LAGRDRYVGWEKPERGR"
     gene            complement(2968..>3361)
                     /locus_tag="VF357_00985"
     CDS             complement(2968..>3361)
                     /locus_tag="VF357_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX7522749.1"
                     /translation="LREEAGGREIRQGVDSPMGGEDDDPLVVQCQDEKSQLKNKSKAL
                     KILRSRLYDAEMAKRQSERADLRRSQVGTGDRSERIRTYNFPQNRVTDHRIGLTMHQL
                     SSVLDGGFDTFIDALTSQAQVEALRKTG"
BASE COUNT          551 a         1238 c         1060 g          512 t
ORIGIN      
        1 gggcacatac acgcccgcgc gtgcgaccgg caggacccgc tgaccgaggg tcgccccgga
       61 gagggagacc ccgaacccgc cttcgacctg gcgccggtga aacgcctcga tccgctccgc
      121 cgccagctcg agcgaccgac gcaacgccgc cggaacgcgc cgccacgccg cgtcgacctg
      181 ccgcggcgga acggcgaacc ccttcctccc ggggtcgaac cggtcgaacc gtcgcgtgta
      241 ctccgccagc gccgcgtcgc cgccttcccg aacctttcgg aggatttccc tgaccaccgg
      301 tgacaccttc tccgcgccgg cggaaccgcg aacctccgcc ctttctagcg cctcccggaa
      361 tcccgccgtt cccgaccggt gccatcgcat gcgtcccgcc tcctattcct tcacccgggc
      421 gatgtcggcg cccagggagg aaagcctgtg ttccagcgcc tcgtacccgc ggtcgaggtg
      481 gtagatccgg agcacctcgg tcgtccccga cgcggccagt cccgccaaca ccagcgaagc
      541 cgaggcgcga aggtcggtcg ccatcagcgg cgccccggaa agcgccggga cccccttcac
      601 tgcggcggtg tttcccgaaa cgtcgattct cgctcccatc ctctgtagtt cggccacgtg
      661 catgaaccgg ttttcgaaaa tcgtctcgga aatgatgctg aagccgtccc ccaggcacat
      721 gtaggccatg aactgcgcct gcacgtccgt gggaaacccc gggtacggag cggtcttgac
      781 gttcacggag cggatcggcc cctctctgcg gacgcgcact cctcccggga cggagtccac
      841 atcggccccg ctactccgaa gcttttcgac gacggcgttc atcgacgagg cgtccgcgcc
      901 ctgcgccgtc acgtctccgc ccgtgatcgc gccggccagg agcagcgtcg ccgcctcgat
      961 cctgtccgcc atcacctcgt ggcgcgcccc gtggagagag cggacccccc ggaccacgat
     1021 ctcgtcggtt ccttccccct cgatgtcggc ccccatggcg cgcagcagcc gcgcgagatc
     1081 gcacacctcg ggctccagcg ccgcgttcga aaggacggtc gtcccctcgg cgagggaggc
     1141 ggccatcatc aggttctccg tcccggtgac ggttttcatg tcgaaggaga cggcggtgcc
     1201 cgaaagacga tccgccgacg cctcgatgta cccctcggag agagaggtct tcgcccccat
     1261 gagctccagc ccccgcaggt gctggtcgat cgggcgggcg ccgatcgcac atccaccggg
     1321 aagggagatc cgggcatgtc cataccgggc gagaagtggc ccgaggacca ggaccgatgc
     1381 gcgcatcgtc ttcaccaact cgtagggcgc ctcggccttc cgggcatccc cgggcgcgat
     1441 cctcaacacc tcgcgcccgt ccgcgccgcc gtcgtcgtgc gtgacctccg cgccgagaat
     1501 cccgagaagc ttcgcgaacg tgtcgatgtc ccgcagccgc ggcgccccga cgacttctac
     1561 cggtccttcc gcgagcagcg acgccgccat ggccggcagc acggcgtttt tcgctccgga
     1621 gacacggcag gtccccgaaa gccgcctgcc ccccttgatg acgaagattt ccagtgtcag
     1681 cgcccccgtt ccggtttttc ccaaccgacg taccggtccc gccccgcgag gtccggaaag
     1741 acccccagcg gccgcaggga accgcacggc agcgaggaca ccgcctcccc ctgcgaagcg
     1801 ccgatttcgc accaaagctc cccaccgggc gacaggagcc cggcggcgcc tgcgaccagg
     1861 ggccgcagga ccgacaggcc gtcccggccg gcaagaagcg cccccggcgg ctcgtggtcg
     1921 cgcacctcgg gtggcaggag gggccactcc ccctcggcga cgtacggtgg gttcgagacc
     1981 actacaacaa aacggccccc gcatttcaag gcggaaagca ggtcgcagca gatgggtcgg
     2041 acgcgggacg ccacgccgtg gcgcgccgcg ttctcgcgca cctttcgaag ggcgccaggc
     2101 gagatgtcta tggccacgac gcaggcggcg ggaacctccg cggcaagggt caccgcgatc
     2161 gccccgctcc cggtccccac gtcgagcgcg aggggtcgcg ccgtcgggga acgtcgaagg
     2221 gcggccactg cccgttccgc cagcccctcc gtctcgggac gcggaataag cgtttcgggg
     2281 gagagcagga actcccttcc gaagaaatcc caggcgccga gaacgtactg gagcggctcg
     2341 ccgccacagc gacgcgcgat cagcgggaac agccgcccct ccgaatcgcc gacgtcatcg
     2401 tccatcgaaa ggaagagtcg gctccgggga atcccggtga ccgcggagac gaggatctcc
     2461 gcctcgccgg gggcggcgtc ccgtacgccc gccatctccc ggcggcagac catgaggagt
     2521 tccgacaggc gcatgggatc gaacctttcg aggatcaacg cctccggcgc ttttttccac
     2581 ttcgaatata cggcaacaaa tgaccatcaa ggagcaggac tccgggctcg cggggggcct
     2641 tcctcttcgc tacggctcgt cgaccctcca tggtctcctc gcctgcgcct ccgcttgctc
     2701 ccgacgtccc tgtctccgct gcgctccaga cgccgcgaag taggaagccc cccgctgcgc
     2761 cctgcgtccc tgaacacggt ggcgcatcgg agcgggatac cctttccgag cggccgtctg
     2821 tttttcccgg agccaaggaa ggcgagaggg aaaaacagcg gcagtgcagg cagccaggga
     2881 cggctgccgg aacgtagctc ggaagggtgt atcacgcgaa gatcaaagcg cctccggata
     2941 cgtgatcgta tctattaacg gaagcgctca gccggtcttg cggagcgcct cgacctgggc
     3001 ctgggacgtc agggcgtcga tgaaagtgtc gaacccgccg tcgagcaccg aggagagctg
     3061 gtgcatcgtc agcccgatcc ggtggtcggt cacgcgattc tggggaaaat tgtaggtgcg
     3121 gatccgttcg ctgcggtccc ccgtccccac ctgcgaccgg cgcaggtcgg cccgctcgga
     3181 ttggcgtttc gccatttccg cgtcgtagag gcgcgagcgg aggatcttca aggccttcga
     3241 cttgtttttc aactgggact tctcgtcctg gcactggacg acgaggggat cgtcatcctc
     3301 cccacccatc ggggaatcta ctccctgccg gatttctcgg ccgccggctt cctcgcgaag
     3361 g
//
LOCUS       DASVJL010000056         7347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1040156, whole genome shotgun sequence.
ACCESSION   DASVJL010000056 DASVJL010000000
VERSION     DASVJL010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040156"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="gatB"
                     /locus_tag="VF357_00990"
     CDS             complement(<1..842)
                     /gene="gatB"
                     /locus_tag="VF357_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX7522750.1"
                     /translation="MAFETVIGLEVHAQLSTRSKIFCACSTAFGAAPNGNTCPVCTGM
                     PGVLPVLNRKAVEFTIRTALATSCTVQRRSVFARKNYFYPDLPKAYQISQYELPVALG
                     GHVDIEVDGGTKRIGITRIHMEEDAGKLVHEGEFAGAQASLADLNRCSVPLMEVVSEP
                     DMRTPEEGGAYLRRLHDILVYLGVCDGNMEEGSFRCDANVSVRPVGQVAFGTKAELKN
                     MNSFRNVEKALEYEIRRQVELIEDGGKVVQETRLWDANAGVTVSMRRKEEAHDYRYFP
                     DPDLL"
     gene            complement(849..2324)
                     /gene="gatA"
                     /locus_tag="VF357_00995"
     CDS             complement(849..2324)
                     /gene="gatA"
                     /locus_tag="VF357_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX7522751.1"
                     /translation="MANVPVHEWTLLEAARKVREKEISSRELTGVLLARIEALDPKVH
                     AYITVLPEAAAAQAAACDEEQAKGALRGPFHGVPIGLKDIFCTRGVRTTCGSRILHDF
                     IPPYDACVAEKVLAAGAVVPGKHNMDEFAMGSSTETSFFGPTRNPWDLSRIPGGSSGG
                     TAAAVAAAMCFAGVGTDTGGSIRQPASLCGVVGIKPTYGRVSRYGMIAFASSLDQGGP
                     IARTVADAAALLGVIAGYDRRDSTCVDIPVPDYLAAVERPVKGLRVGLPKEYYEGEGL
                     SPEVRAAVEKALKALTDQGAIVVEVSLPHTSFALSTYYLVAPAEASSNLARYDGVRYG
                     HRAGGASGLIEMMSRTRAEGFGDEVKRRIMIGTYALSAGYYDAFYGKAQKVRTLIRGD
                     FTKAFEAADVLLTPTAPTPAFRLGEKTGDPLTMYLSDIYTIPCNLAGIPGISVPCGFS
                     AEGLPIGAQLLSKPFREETLFTAAGAIERALPLRRVAPLEG"
     gene            complement(2344..2634)
                     /gene="gatC"
                     /locus_tag="VF357_01000"
     CDS             complement(2344..2634)
                     /gene="gatC"
                     /locus_tag="VF357_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX7522752.1"
                     /translation="MAITRDEVLHVARLARLSLPEVEAERLREQLSAILDDVKQLDRL
                     DTRDVLPTSHAIETGTPFRDDVVEPFGDREALLANAPDRRNDCFRVPRIIED"
     gene            complement(2739..4418)
                     /locus_tag="VF357_01005"
     CDS             complement(2739..4418)
                     /locus_tag="VF357_01005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7522753.1"
                     /translation="MQKNSEFLSLVFNNIRENIVVVNAENYGILHANQSFLESYGISL
                     EESRKTRCYEVTHKNDRPCHESGEECPVRQAADAGRVSKCLHVHKSKEGECRTTRLAA
                     YPIREADGTVRRVVEISEDITEPVRLQEVIRKKSEYLDNILKNSPDGIIGNDPEGNIF
                     LFNESAKGIFGYTAAQAIGKIKASALYPPGGAREVKEFIYAEDFGGRGRLQGFETKIV
                     TSAGKSTPIRLSCELLHEGGREIGTIGFFHDISGRLALQNHLAESEAKFRTLFETASD
                     AILSIDEDGLILMANRAAKDVFEYPGQEIAGLDVRQLLGSGQEGAWEVLARYASRSES
                     GKYVESSAVSRSGKKIPFHITVSENVSGGKRIYTAIMRDVSQIKAYEEDLQILANTDS
                     LTRLYNRRQLYPILQKELDHAVRKKVPFSVLLIDIDHFKKFNDTYGHAGGDLLLVGFA
                     DKIRSAIRQMDSAFRFGGEEFVVLLPEITGQDAMVPAERFRQLIADSRFPMPPGGQPV
                     SVTVSVGIAGYRNGDTLDDLIRHADLAMYAAKSGGRNRVADYDQLTNPETK"
     gene            4592..5722
                     /locus_tag="VF357_01010"
     CDS             4592..5722
                     /locus_tag="VF357_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX7522754.1"
                     /translation="MATHRFFATTFKGLEEVLAGEIAALGGEDVSASAGSVAFSGDMA
                     LCYRANLWLRSANRVVLLLSEFPAPTPAALYEGASGIPWAELFSADRTIAVDATVRDS
                     GITHSHFAAQKTKDAVVDRFRETVGRRPDVDAKSPEVRIVVRIVRDECAVSLDTSGES
                     LNRRGYRANPSEASLKETLAAGLLLLTGWQGDEPLIDPACGAGTIPIEAALIAGNVAP
                     GSLGRDFGFQRIHGFDRNRWEALLSEAREAARHPVPVRIEGSDISPAAVAGAMKNAIA
                     AKVNERVLFNARSIRNLDPGEGPGILLCNPPYGVRLPGGEEVEAFYREMGEALKKRCR
                     GWTAYLLSGNSAVTRFLGLKASRKFPVINGPIDCRLLKYELY"
     gene            complement(5724..6215)
                     /locus_tag="VF357_01015"
     CDS             complement(5724..6215)
                     /locus_tag="VF357_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522755.1"
                     /translation="MSDERVLPDVSRGDFLKLAGLSALLPLIGSVTPASAAGGKERLY
                     VVTHADDDVDRAALALLLAMVDAKKIGKELPGVTIWFTLPGVKLCRKGAAEKLTSPIF
                     KKFGTLADILGGAVKAGAKLGGCPFCLDFFGIPKSEYVDGLDRKGGDYVAAQVGKADV
                     IWM"
     gene            6437..6847
                     /gene="tsaA"
                     /locus_tag="VF357_01020"
     CDS             6437..6847
                     /gene="tsaA"
                     /locus_tag="VF357_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEX7522756.1"
                     /translation="MAEYIVRPIGVVRSMLKDPADAPKQGALTGQEAEIVVDPAYLPA
                     LEGLDRGRGKIIVMCWMDRADRGRLKVHPQGQEGLPERGVFSTRSPHRPNPVSLHTVT
                     LLSTEGNVLRVRGMDAIDGTPVVDIKIHSPELDG"
     gene            complement(6875..>7347)
                     /locus_tag="VF357_01025"
     CDS             complement(6875..>7347)
                     /locus_tag="VF357_01025"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522757.1"
                     /translation="NPEEGELRPQLLDRFGLCVEISGERDVGFRKSIVERVLLFEGED
                     AGFQEKWDRKDEELQARLVAARAALPRVEVPGEILESIVAVVGELGVAGHRGDITVLK
                     ASKALAAIKGIPSPDEECLSDAFRLALPHRLKEDPFEETASGRKRLDGVLARFG"
BASE COUNT         1253 a         2438 c         2331 g         1325 t
ORIGIN      
        1 agcaggtccg gatcggggaa gtaccggtag tcgtgcgcct cctccttccg gcgcatcgac
       61 acggtgacgc ccgcgttcgc gtcccaaagc cgcgtctcct gcaccacctt ccccccgtcc
      121 tcgatcagct cgacctgccg ccgtatttca tactcgagcg ccttctcgac gttgcggaag
      181 gagttcatgt ttttcagctc ggccttcgtg ccgaacgcca cctgcccgac cggacgaacc
      241 gagacgttcg cgtcgcaccg gaacgaccct tcttccatgt tcccgtcgca caccccgagg
      301 tagacgagga tatcgtgcag gcgccgcagg tacgcgccgc cctcctccgg cgtgcgcatg
      361 tcgggctcgg agacgacctc catcaggggg acagagcatc ggttgagatc cgcgagcgac
      421 gcctgcgcgc cggcgaactc cccttcgtgg acgagcttcc cggcatcctc ctccatgtgg
      481 atccgcgtga tcccgatccg cttcgttccc ccgtcgacct caatgtcgac gtgaccgccg
      541 agggcgaccg gcagctcgta ctgcgagatc tggtacgcct tggggaggtc cgggtagaag
      601 tagttcttcc gggcgaagac gcttctgcgc tgcaccgtgc aggaggtagc gagcgccgtc
      661 cggatcgtga attccaccgc cttgcggttg agcaccggga gcacgcccgg catgccggtg
      721 cacacggggc aggtgttccc gttgggggcg gcgccgaacg ccgtggagca ggcgcagaag
      781 atcttgctcc gcgtggagag ctgggcgtgg acttccagcc cgatgaccgt ctcgaacgcc
      841 atcgcttcct atccctccag cggcgccacg cgccgcaacg gaagggcgcg ctcgatcgcg
      901 ccggcggcgg tgaacagcgt ctcctcccgg aaaggtttcg agagtagctg cgccccgatc
      961 ggaagtccct ccgcggaaaa gccgcaaggg acggagatcc ccgggatacc ggccaggttg
     1021 cacgggatcg tgtagatgtc ggacaggtac atcgtgagcg gatcgccggt cttctccccg
     1081 aggcggaacg ccggcgtcgg ggcggtgggg gtgaggagaa cgtccgccgc ctcgaacgct
     1141 ttcgtgaaat cgccccggat caaggtgcgg accttctgcg ccttcccata gaaggcgtcg
     1201 tagtagcccg cggagagcgc ataggtgccg atcatgatgc ggcgcttcac ttcgtcgccg
     1261 aacccttccg cccgcgtccg cgacatcatc tcgatcaggc ccgaggcgcc tcccgccctg
     1321 tggccgtacc ggacgccgtc gtaccgcgcg aggttggacg acgcctccgc gggagcgacg
     1381 agatagtacg tggagagggc gaacgaagtg tgcggcagcg agacctccac cacgatggcg
     1441 ccctgatcgg taagggcctt caacgctttt tccaccgccg cacggacctc gggggaaagt
     1501 ccctcgccct cgtaatactc tttcgggagg ccgacccgaa gccccttgac cgggcgctcc
     1561 accgcggcga ggtagtccgg caccgggatg tccacgcagg tcgaatcgcg ccggtcgtac
     1621 cccgcgatca cgccaagcag cgccgccgcg tcggcaactg tccgcgcgat cgggccgccc
     1681 tggtcgagcg aagacgcgaa ggcgatcatc ccgtagcgcg acacccggcc atacgtcggc
     1741 ttgatgccca ccacgccgca cagggaggcc ggctggcgga tcgacccgcc ggtatcggtc
     1801 ccgacgccgg cgaagcacat cgcggcggcc accgccgccg ccgttccccc cgaggagccg
     1861 cccgggatgc gcgacaggtc ccacgggttg cgggtgggac cgaagaagga ggtctccgtc
     1921 gaggagccca tcgcgaactc gtccatgttg tgctttcccg gcacgaccgc gccggcggcg
     1981 aggacctttt ccgccacgca ggcgtcgtag ggggggatga agtcgtgcag gatccggctc
     2041 ccacaagtgg tgcgcacgcc gcgggtgcag aagatgtcct tgagcccgat cggaacgccg
     2101 tgaaacgggc cgcggagggc ccccttcgcc tgctcctcgt cgcacgccgc ggcctgcgcc
     2161 gccgcggctt ccgggaggac ggtgatgtac gcgtggacct tcggatcgag cgcctcgatc
     2221 cgcgcgagga gcacgccggt cagctcgcgg gaggagatct ccttctcccg cacctttcgc
     2281 gccgcttcga ggagggtcca ctcgtggacg ggaacgttcg ccatgcggtt tgccggaccc
     2341 ttcctaatct tcgatgatcc gcgggacgcg gaaacagtcg ttccgccggt cgggggcgtt
     2401 cgcgaggagc gcctccctgt ccccgaacgg ttccaccacg tcgtcgcgga agggggtgcc
     2461 cgtctcgatg gcgtgggacg tggggaggac gtcacgggtg tcgagccggt cgagctgctt
     2521 cacatcgtcg aggatggccg agagctgctc gcgaaggcgc tccgcctcca cctccggcag
     2581 cgaaaggcgc gccagtcggg cgacgtgcag cacctcgtcc cgcgtgatcg ccatgggatc
     2641 tcccccggtg gaatgaaaaa atgaatttac cataccgaca gggggggagg caaaacacct
     2701 cgcgtcctct tggcgagtgc gctccgcctc ccgaatctct acttcgtttc cggattggtc
     2761 aactggtcgt agtcggcgac cctgtttctc ccgccgcttt ttgcggcgta catcgcgagg
     2821 tccgcatgcc ggatcagatc gtcgagcgtg tccccgttcc ggtacccggc gatgcctacg
     2881 gagacggtga ccgacaccgg ctggcctccc gggggcatgg gaaaccggct gtcggcgatg
     2941 agttgacgga accgctcggc gggaaccatc gcatcttgcc ccgtgatttc cgggaggaga
     3001 acaacgaact cctccccgcc gaaccggaag gcggaatcca tctgccggat cgccgacctt
     3061 atcttatcgg caaaacccac cagcaacagg tctccccccg cgtggccgta cgtgtcgttg
     3121 aacttcttga agtggtcgat gtcgatcagc agaaccgaga acgggacctt tttcctgacc
     3181 gcatggtcga gctccttctg caggatcgga tagagctgtc gtcggttgta gaggcgggtc
     3241 agactatcgg tgttcgcaag aatctgcaga tcctcctcgt acgccttgat ctgcgacaca
     3301 tcccgcatga tggcggtata gatcctcttt ccaccgctga cgttttcgga aacggtgatg
     3361 tggaagggga tcttcttgcc ggatctcgac acggcggaag attccacgta cttccccgac
     3421 tccgagcgcg aggcgtacct ggcgagaacc tcccacgctc cttcctgccc cgatccaagc
     3481 agttgacgga cgtccaatcc ggcgatttcc tgacccggat actcgaatac gtctttggcc
     3541 gccctgttcg ccatgagaat gagcccatcc tcgtcgatgc tgaggatcgc gtcactggcc
     3601 gtctcgaaca gagtccggaa tttcgcttcc gactccgcca gatggttctg cagcgccaat
     3661 cgaccggaaa tgtcatggaa aaaaccgatc gtcccgatct cccgaccgcc ttcatggagc
     3721 aactcgcagg atagcctgat gggagtcgat ttccccgcgc ttgtcacgat cttcgtttcg
     3781 aaaccctgga gcctgccgcg gccgccgaaa tcttcggcgt agatgaactc cttgacttcc
     3841 ctcgcaccgc cgggcgggta gagcgcagag gctttgattt tcccgatcgc ctgggcggcc
     3901 gtgtatccga aaatcccctt cgcgctctca ttgaagagga atatgttccc ctcggggtcg
     3961 ttcccgatga tcccgtccgg gctgttcttc aagatgttgt cgagatattc cgacttcttc
     4021 cgtatcactt cctgcaacct cactggttcg gtaatgtcct cggatatttc cacgacacgg
     4081 cgaaccgttc cgtcggcctc ccggatcgga tacgccgcaa ggcgggtagt ccggcattcc
     4141 ccttctttac tcttgtggac gtgtaggcac ttggagacgc gtcccgcatc cgctgcctgg
     4201 cggaccgggc actcctcccc ggattcgtgg cacggtcgat cgttcttgtg ggtcacctcg
     4261 taacaacgcg ttttccgact ttcttccagg gaaatgccat acgactcaag aaatgactgg
     4321 ttcgcgtgga gaatcccgta attttccgcg ttcacgacaa cgatgttctc gcggatgttg
     4381 ttgaacacca gcgagagaaa ttcggaattt ttctgcatcc cgcccttttc caaaacatga
     4441 ttttatccgg ccttggttga tctttcggaa atatccgcaa agatcttcac atgatcttgc
     4501 acttttctgc cgatgtccct tattcgccaa ggcgcctgtt gccgcggagc gtgcgacgca
     4561 gataggtttg gattcagagt atcatgctcc aatggcgacg caccggtttt tcgcgacgac
     4621 gttcaagggg ttggaagaag tgctggcggg ggagatcgcg gccctcggcg gggaagacgt
     4681 ttccgcgagc gccggatccg tcgctttctc cggcgacatg gcgctctgct accgcgccaa
     4741 cctgtggctc cggtcggcca accgggtcgt cctgctcctg tcggagttcc ccgcgccgac
     4801 tcccgcggcc ctctacgagg gggcaagcgg gatcccctgg gcggagttgt tctcggcgga
     4861 tcggacgatc gcggtcgacg ccacggtgcg cgactccggc attacccact cccatttcgc
     4921 ggcccagaag acgaaggacg cggtcgtcga ccggttccgc gagacggtcg ggcggcggcc
     4981 ggacgtggac gcgaagtcac cggaggtgcg gatcgtcgtc cggatcgtgc gcgacgagtg
     5041 tgccgtctcg ctcgacacct cgggggagag cctgaaccgg cgcggctacc gggcgaaccc
     5101 gtcggaggcg tcgctcaagg agacgttggc cgcggggctt cttctgctca ccgggtggca
     5161 gggggacgag ccattgatcg acccggcgtg cggcgccggg acgatcccga tcgaggcggc
     5221 gctgatcgcg gggaacgtag cgccggggtc cctggggcgc gacttcgggt tccagcgcat
     5281 ccacgggttc gaccggaacc ggtgggaggc gctcctctcg gaggcgcggg aggcggcccg
     5341 tcatccggtc ccggtgcgaa tcgagggaag cgatatttcc ccggccgcgg tggccggggc
     5401 gatgaagaac gccattgctg caaaagtaaa cgaacgggtt ctatttaacg caagatccat
     5461 ccgcaatctc gatcccgggg aggggccggg gatcctcctg tgcaaccctc cgtatggcgt
     5521 ccgcctcccc ggcggcgaag aggtcgaggc gttctatcgg gagatggggg aggcgctcaa
     5581 gaaacggtgc cgggggtgga cggcgtacct gctctccggc aactccgccg tcacccgctt
     5641 cctcggcctg aaggcgtcac ggaagttccc cgtcataaac ggcccgatcg actgccgcct
     5701 gctgaagtac gaactgtact gaactacatc cagatcacgt cggccttccc cacctgcgcc
     5761 gccacgtaat cgcctccctt gcgatccagc ccgtccacat actccgattt cgggatcccg
     5821 aagaagtcga ggcagaacgg gcaaccgccc agtttcgccc ccgccttcac ggcgccgccg
     5881 aggatgtcgg ccagggtccc gaacttcttg aagatcgggg aggtgagttt ttccgcggcg
     5941 ccttttctgc acagcttcac ccccggaagg gtgaaccaga tcgtgacgcc gggaagctcc
     6001 ttgccgatct tctttgcgtc gaccatcgcc aacagcaggg cgagggccgc gcggtccacg
     6061 tcgtcgtccg cgtgggtcac cacgtagaga cgctcctttc cgcccgccgc ggacgcggga
     6121 gtgaccgaac cgatcagggg aaggagggcg gagaggccgg ccagtttcag gaagtcaccg
     6181 cgggaaacat ccggaagaac gcgttcgtcg gacattggaa gagatctcct ttcgcctcgg
     6241 aaattgggac gacgaatcgt ttcgcgttcc ccgccgaccg gcggagcggc cacccgcgtc
     6301 tcagcgtacg cggcggcgca aaggcatcga atccgccaag gagatcacct cccttccttc
     6361 atcgaatgat tggtgtatct tagcaaatgc accgtggggg ggcatcacgg aagccggggg
     6421 gcgaagggaa gaccggatgg cggagtacat cgttcggccg atcggtgtcg ttcggtcgat
     6481 gttgaaagac ccggcggacg cgccgaagca gggggcgctg accggccagg aggcggagat
     6541 cgtcgtcgat ccggcgtacc ttccggcgct ggaagggctt gaccgcggga gggggaagat
     6601 catcgtgatg tgctggatgg accgggccga ccggggccgc ctgaaggtcc atccgcaagg
     6661 acaggaggga ctgccggagc gcggcgtctt ctcgacccgc tcgccgcacc ggcccaaccc
     6721 ggtttcgctc cacaccgtca cgctgctctc gaccgaaggg aacgtgctgc gcgtccgggg
     6781 tatggacgcg atcgacggca ccccggtggt cgacatcaag atccactccc cggaactgga
     6841 cggctgatcc tttcgcgaac ggagagggga aggactaccc gaaccgggcc agcacgccgt
     6901 cgaggcgctt tcgcccggat gccgtctcct cgaacgggtc ttccttcaat cggtgcggca
     6961 acgccagccg gaaggcgtcg gagaggcact cctcgtcggg cgacgggatc cccttgatcg
     7021 ccgccagcgc cttggacgcc ttcagcaccg tgatgtcccc gcgatgcccg gccacgccca
     7081 gctccccgac cacggccacg atcgattcaa ggatctcccc gggaacctcg acgcggggga
     7141 gggccgcccg ggccgccacc agccgagcct gcagttcctc gtccttgcgg tcccacttct
     7201 cctggaaccc cgcatcttct ccctcgaaca gcaggacacg ctccacgatc gacttccgga
     7261 acccgacgtc ccgctccccg gagatctcca cgcacaaccc gaaccggtcc agcagctgag
     7321 gccgcaactc cccctcttcc ggattca
//
LOCUS       DASVJL010000057         3729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_7889, whole genome shotgun sequence.
ACCESSION   DASVJL010000057 DASVJL010000000
VERSION     DASVJL010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7889"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VF357_01030"
     CDS             complement(<1..339)
                     /locus_tag="VF357_01030"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7522758.1"
                     /translation="MRVLVAGGAGFIGSNFIRYILGAHGDWSVVNVDKLTYAGNLANL
                     ADMAGNPRYRFHRADICDAAEVARIFAEEKPEAVVNFAAETHVDRSIDDPALFLRTNI
                     LGTQVLLDAAR"
     gene            complement(339..1199)
                     /gene="rfbD"
                     /locus_tag="VF357_01035"
     CDS             complement(339..1199)
                     /gene="rfbD"
                     /locus_tag="VF357_01035"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX7522759.1"
                     /translation="MKVLITGGLGLLGKEIARVFEGSAGIRSTDREEWDVTDPAACRR
                     EIAGFRPDVVVHCAAYTAVDRAESDPETARILNVEGTRNVARACRELGAVMVTFGTDY
                     IFDGASSRPYREEDPANPLSVYGKTKWAAEQALREEGGDQLLVRTQWMFGPAGKNFIP
                     TILEKARKGDALRVASDQVGCPTFSRDLAGAVRNLLGVRARGTVHFSSEGETSWFGLA
                     RYVLERCGLPAALLSPARTRDLPYPAPRPAYGVLSKEKYRAVTGVSPRPWEEAVGEYL
                     EMIDQEGGTR"
     gene            complement(1231..1686)
                     /locus_tag="VF357_01040"
     CDS             complement(1231..1686)
                     /locus_tag="VF357_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEX7522760.1"
                     /translation="MIDGVMVKQLRVLPDERGRLMEILREDDEIYMRFGQVYLTTGYP
                     GVVKAWHYHKVQFDHFCVVKGMMKVVLYDSRDGSPTKGEVNEFFLGEHRPIVLRIPPL
                     VYHGFKTISEEEAFLINVSTQTYKYDVPDEFRLPPHDNDIPYDWARKDG"
     gene            complement(1718..3088)
                     /locus_tag="VF357_01045"
     CDS             complement(1718..3088)
                     /locus_tag="VF357_01045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX7522761.1"
                     /translation="MNVAIVGTGYVGLDTGVCLAERGNTVHCVDTNPVVVEKLNAGQV
                     TIYEPGLDDIFLRNLKKGRIAFSGDLEKAVLPAEIIFLCLPTPPGDDGSADLKYILQV
                     ADDLGKIFARNPGAGYKVVVDKSTVPVRTSDKVQAAILKNAGPAFEFNVVSNPEFLRE
                     GFAVEDFLRPERVVIGSSEERAIVALKDLYEPFLQTGSKVIVMDEKSAEVTKYAANAF
                     LALKISYMNDLANFCDAVGADIDRIREGIGSDSRIGPKFLHPGLGYGGSCLPKDVKAL
                     LKSASDAGTPLSVLQAVEDINQEQRKRFFRRMERHFGDKLEGLRFAVWGIAFKPNTDD
                     TREAPVFHLIDGMLNAKASVVVFDPEAMGGARARYGDRIEYAESSYGALQGADVLVIA
                     TEWFEFRKPDFGLMKSLLRQPVIFDGRNIYDPRKMRERGFLYHSIGRKAYEGHAANGT
                     PEPGRS"
     gene            3289..>3729
                     /locus_tag="VF357_01050"
     CDS             3289..>3729
                     /locus_tag="VF357_01050"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX7522762.1"
                     /translation="MTAIPPGTAHAIGVPLPVTVTVEGEVRRPGSYTLPRGATLSTLI
                     VAAGGYTDNADLAAATLKRESAKTVAAPLSHPRLLKGSPADLPLTDGDTLRIPTRTPG
                     APARTRARPAGDEPFTGPNNFGVTGLFETPTARVMEENRYRLGAT"
BASE COUNT          608 a         1370 c         1102 g          649 t
ORIGIN      
        1 gcgcgccgcg tcgagcagca cctgcgtccc caggatgttc gtccgcagga agagcgccgg
       61 gtcgtcgatg ctgcggtcca cgtgcgtctc ggcggcgaaa ttcacgaccg cctcgggctt
      121 ctcctccgcg aagatccgcg ccacctcggc cgcgtcgcag atgtcggccc gatggaagcg
      181 gtaccgggga ttcccggcca tatccgccag gttcgcgagg ttccccgcgt aggtcagctt
      241 gtccacgttc accacggacc aatcgccgtg ggccccgagg atgtaccgta tgaagttcga
      301 gccgatgaaa ccggccccgc ccgcgacgag aacccgcatc agcgcgtgcc cccttcctga
      361 tcgatcatct caaggtactc ccccaccgct tcctcccacg gcctcgggga gacccccgtg
      421 accgcgcgat acttctcctt gctcaaaacg ccatacgccg gccttggcgc cgggtacggc
      481 aagtcccgtg ttcgcgccgg ggacagcagg gcggcaggca acccgcatcg ctccagcacg
      541 taccgggcga ggccgaacca gctcgtctcc ccttcgctcg agaagtgaac cgtcccccgc
      601 gcgcgcacgc cgagcaagtt acggacggcg cctgccaggt ccctcgaaaa cgtcgggcac
      661 ccgacctggt cggacgcgac gcgaagcgca tcccccttcc gtgccttttc gaggatcgtg
      721 gggatgaaat tcttccccgc ggggccgaac atccactggg tccgcaccaa cagttggtcg
      781 cccccctcct cccgaagcgc ctgctcggcc gcccacttcg tctttccgta cacggagagc
      841 gggttcgccg gatcctcttc ccgatacggg cgcgaggaag caccgtcgaa gatgtagtcg
      901 gtgccgaacg tgaccatcac ggccccgagc tctcggcagg cgcgcgcgac gttgcgcgtc
      961 ccctcgacgt tgaggatgcg cgccgtctcc ggatcgctct ccgcccggtc cacggccgtg
     1021 taggccgcgc aatggaccac cacgtccggg cgaaacccgg cgatctcccg ccggcacgcc
     1081 gcggggtccg tcacgtccca ctcttcccgg tcggtcgagc ggatcccggc ggacccttcg
     1141 aagacccgcg cgatctcttt cccgaggagg ccgagcccgc cggtgatcag cactttcacg
     1201 cggtgggccc ccgcgccccg ccctcgacgg tcacccgtcc ttcctcgccc aatcgtaggg
     1261 gatgtcgttg tcgtgcgggg gaagacggaa ctcgtccggc acgtcgtact tgtaagtctg
     1321 cgtggagacg ttgatcagga acgcctcttc ctcgctgatc gtcttgaagc cgtggtagac
     1381 gagcgggggg atccgcagga cgatcggccg gtgctcgccg aggaagaatt cgttcacctc
     1441 ccccttcgtg ggggagccgt cccgggaatc gtacaacacc accttcatca tccccttgac
     1501 cacgcagaag tggtcgaact ggaccttatg gtagtgccac gccttgacga cgcccgggta
     1561 cccggtggtc aggtacacct ggccgaaccg catgtagatc tcgtcgtcct cgcgcaggat
     1621 ctccatcagc cgcccgcgtt cgtccgggag caccctcagc tgcttcacca tcacgccgtc
     1681 gatcatctca ccctcctgac aaacttccgt gggagttcta ggaacgtccc ggttccgggg
     1741 tcccgttcgc cgcgtgcccc tcgtaagcct tgcggccgat cgagtggtag aggaagccgc
     1801 gctcccgcat cttccgcggg tcgtagatgt tgcgcccgtc gaagatgacc ggctgccgca
     1861 acagggactt catcagcccg aaatcgggct tgcggaactc gaaccactcc gtggcgatca
     1921 ccaggacatc cgccccctgc agcgcgccgt acgacgactc cgcgtactcg attctgtccc
     1981 cgtaccgcgc ccgcgcgccc cccatcgcct ccgggtcgaa gaccaccacc gacgccttgg
     2041 cgttcagcat cccgtcgatc agatggaaga ccggcgcctc ccgcgtgtcg tccgtgttcg
     2101 gcttgaacgc gatcccccag acggcgaacc tcagcccctc gagcttgtcg ccgaaatgcc
     2161 gctccatcct cctgaagaag cgcttccgct gctcctggtt gatatcctcc accgcctgca
     2221 ggaccgagag cggcgtcccc gcgtcggacg cgcttttcag caacgccttc acgtctttcg
     2281 ggagacacga cccgccatac cccagccccg ggtggagaaa cttgggcccg atgcgagagt
     2341 ccgagccgat cccctcgcgg atcctgtcga tgtcggcccc cacggcgtcg cagaagttcg
     2401 ccaggtcgtt catgtaggag atcttgaggg cgaggaaggc gttcgccgcg tacttggtca
     2461 cctcggcgct tttttcgtcc ataacgatga ccttgctgcc ggtctgcagg aacggctcat
     2521 aaaggtcctt gagggccacg atcgcccgct cctcgctcga cccgatcacc acgcgctccg
     2581 gccgcaggaa atcctcgacc gcgaacccct cgcgcaggaa ctccgggttc gacacgacgt
     2641 tgaattcgaa cgccgggccc gcattcttaa ggatcgccgc ctgcaccttg tcgctcgttc
     2701 tcaccggcac ggtggatttg tcgaccacca ccttgtaccc cgcgcccggg ttgcgcgcga
     2761 agatctttcc gagatcatcc gccacctgga ggatgtattt caggtccgcg gagccgtcgt
     2821 ccccgggggg cgtgggcagg cacaggaaga tgatctcggc aggcaggacc gccttctcga
     2881 gatcccccga gaacgcgatc cgcccctttt tcaggttgcg caggaagatg tcgtcgagcc
     2941 cgggctcgta gatcgtcacc tgtcccgcgt tcagcttctc gaccactacc gggttcgtgt
     3001 cgacgcagtg cacggtgttc ccccgctctg cgaggcatac cccggtatcc agccccacgt
     3061 atcccgttcc gacgatcgcg acgttcatcc gtcacccccc atcatttcaa atatcttggg
     3121 attccgagtc tcctgatcgg ctgagagtac cttaaacttc agtgcacggg aagtccggaa
     3181 gcgaaccggc cgacaccggg gtatacttta aaaatgccct gcatcctccg aagcgtccgc
     3241 cgccgcctgg gtgcgtggat cgcttcctgt ctcctgctgg gagccgtagt gaccgcgatt
     3301 ccgccgggga ccgcccatgc gatcggggtg ccgctgccgg tcacggtcac cgtggaggga
     3361 gaggtccggc gtcccggcag ctacacgcta ccgcgcggcg cgacgctctc caccctgatc
     3421 gtcgcagccg gcggatatac cgacaacgcg gacctcgcgg cggcgaccct gaagcgggaa
     3481 tccgcgaaaa ccgttgccgc tcccctctcc catccacggt tgctgaaggg aagcccggcg
     3541 gacctgccgc ttacggacgg cgacacgctc cgcatcccga cgcggacacc gggcgccccg
     3601 gccaggacgc gcgcccgccc cgccggcgac gagccgttca ccggcccgaa caacttcggc
     3661 gtgaccggcc ttttcgaaac ccccaccgca cgcgtcatgg aggagaaccg gtaccgcctc
     3721 ggcgccacg
//
LOCUS       DASVJL010000058         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_477299, whole genome shotgun sequence.
ACCESSION   DASVJL010000058 DASVJL010000000
VERSION     DASVJL010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477299"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VF357_01055"
     CDS             <1..886
                     /locus_tag="VF357_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7522763.1"
                     /translation="MIDVIYNPVAGPRVVNRIDQVRSYLSARGRPFRIRETTAPGDAV
                     VMAREAALEGADAVVAVGGDGTMSEVADGLAGSATRLAFVPHGTGNVFASEFSLPESV
                     EECLDLLSSGKTISVRMAKANDRRFVLLASAGFDAEVVERMNPRQKNLLGIAAYVLCG
                     ARHLLRSQPTLWLEFPDRERVEAQAVLVVRGKKYGGNVTIAPDGDIAGETLQVIALLR
                     KGRWSIAKFALDALRGTHAASRHVLIRESPSLFVRSTIPSASQVDGEYLGPLPVRFTV
                     TDALLRVIVPKEFPFPPA"
     gene            complement(900..1127)
                     /locus_tag="VF357_01060"
     CDS             complement(900..1127)
                     /locus_tag="VF357_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522764.1"
                     /translation="MTRMRGSTSKDLGHRAFGTAGGWKTQDEAPGRSGEGIDLVEETL
                     RKPIVLPASRAWKPRAGRIYNFPVRSIPDEK"
     gene            complement(1124..>2252)
                     /locus_tag="VF357_01065"
     CDS             complement(1124..>2252)
                     /locus_tag="VF357_01065"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7522765.1"
                     /translation="QYGSAWRALGNGRRATEPADDGDLPAVSILKPLKGVDDRLLDNL
                     ESFCRLDYPCYEIVFCVQGASDPALRVARKVKEMHPEREIVVVVGDCQKGLNPKVNNM
                     MPGYAAARYPFVLISDSNVAPAPGYLREAMAHFRDPSVGLVSHLVRGIGAKTLGARLE
                     NQHLNTFILPSVSLLDRMFGMPCVVGKSMLMRREDLDAMGGLASVKDYLAEDYVLGET
                     FRKAGKKVVISGSPVDNVNVYRTPRQFLSRHARWNRMRFSIAGAGYFVELFTNPVGLS
                     LLMVAAAPGDAEAWTIVGGVAAAKMAMDYGMHRMLGDRSSPGWILLGPFRDVLAGGLW
                     FSAFFSRNVEWRGRTLRITRDSRLVPVDEAPAMEPATEAMR"
BASE COUNT          347 a          814 c          719 g          372 t
ORIGIN      
        1 aatgatcgac gtgatctaca accccgtcgc cggcccgagg gtcgtgaacc ggatcgacca
       61 ggtccgctcg tacctgtcgg cgcgcggtcg ccccttccgg atccgggaga cgaccgcccc
      121 cggggatgcc gtcgtgatgg ctcgcgaggc ggcgctcgaa ggggccgacg ccgtggtcgc
      181 cgtcggcggg gacggcacga tgagcgaggt cgcggacggg ttggcgggaa gcgccacccg
      241 cctggccttc gtcccccatg ggaccgggaa cgtcttcgcc agcgagtttt ccctccccga
      301 atcggtggaa gagtgcctcg acctgctctc ctccgggaag acgatctccg tccggatggc
      361 gaaggcgaac gaccggcgct tcgtcctgct ggcctccgcg gggttcgacg ccgaggtggt
      421 ggagcggatg aacccccgcc agaagaacct cctcggcatc gccgcctacg tcctgtgcgg
      481 tgcgcggcac ctcctccgct cccagccgac cctctggctc gagttccccg accgcgagcg
      541 ggtcgaggcg caggccgtcc tcgtggtccg cggcaaaaag tacggcggga acgtgacgat
      601 cgcaccggac ggggacatcg cgggggagac gctccaggtg atcgccctcc ttcggaaggg
      661 gcgctggtcg atcgcgaagt tcgccctcga cgccctgcgg gggacgcacg ccgcctcccg
      721 ccacgtcctg atccgggaga gtccgtccct cttcgtccgc agcacgatcc cctccgccag
      781 ccaggtggac ggggagtacc tcggaccgct cccggtgcgg ttcaccgtga ccgacgccct
      841 cctgcgggtc attgtcccga aggagttccc cttccccccg gcttgacaaa ggtacggtgt
      901 tacttttcgt ccgggatgct tctcacggga aagttgtaga tgcggcctgc gcggggcttc
      961 cacgcccgcg acgccggcag gacgatcggc ttgcgcaggg tctcttccac caggtcgatc
     1021 ccctccccgg accgccccgg cgcctcgtcc tgcgttttcc atccccccgc cgtgccgaaa
     1081 gcgcggtgcc cgaggtcttt cgacgtgctt cctcgcattc gggtcatcgc atcgcctccg
     1141 tggcgggttc catcgccggt gcctcgtcca ccggcaccag tctggaatcc cgggtgatcc
     1201 gtagcgtccg cccccgccac tcgacgtttc gcgagaagaa ggcggagaac caaagacccc
     1261 ctgccagcac gtcgcggaag ggaccgagca ggatccaccc cggggaagag cggtccccca
     1321 gcatccgatg catgccgtag tccatcgcca tcttggcggc cgcaacccca ccgacgatcg
     1381 tccatgcctc ggcatccccc ggggccgcgg cgaccatcag cagcgagagt cccaccgggt
     1441 tggtgaacag ctcgacgaaa tatcccgccc ccgcgatcga aaagcgcatc cggttccacc
     1501 gggcatgtcg cgagaggaac tggcgcggcg tgcggtacac gttcacgttg tccacgggcg
     1561 atcccgagat caccaccttc ttcccggcct tccggaacgt ttccccgaga acgtagtcct
     1621 cggcgagata gtctttcacg gaggccagcc cgcccatcgc gtccaggtcc tcgcggcgca
     1681 tgagcatcga cttgccgacg acgcaaggca tgccgaacat ccggtcgagg agggagacgg
     1741 aggggaggat gaacgtgttc aggtgctggt tttccagtcg cgcgcccagc gtcttcgcgc
     1801 cgatcccgcg gaccaggtgg ctcaccagtc ccaccgaggg atcccggaag tgcgccatcg
     1861 cctcccgcag gtaccccggc gcgggcgcca cgttgctgtc gctgatgaga acgaacgggt
     1921 acctcgcagc ggcgtacccc ggcatcatgt tgttcacctt tggattcagc cccttctggc
     1981 aatcccccac caccacgacg atctcccgtt cggggtgcat ctccttcacc ttccgggcca
     2041 cccgcagcgc cgggtcgctc gccccctgga cgcagaagac gatctcgtag cagggatagt
     2101 ccagccggca gaagctctcc aggttgtcga ggagccggtc gtccactccc ttgaggggct
     2161 tgaggatcga cacggccggc aggtctccat cgtcggcagg ctcggttgcg cggcgaccgt
     2221 tcccgagggc tctccacgcg gagccgtatt gc
//
LOCUS       DASVJL010000059         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_664974, whole genome shotgun sequence.
ACCESSION   DASVJL010000059 DASVJL010000000
VERSION     DASVJL010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664974"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..936)
                     /gene="nuoB"
                     /locus_tag="VF357_01070"
     CDS             complement(223..936)
                     /gene="nuoB"
                     /locus_tag="VF357_01070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX7522766.1"
                     /translation="MTVALPAGDLAAPFRGMPVIVEAFRSGNVAETCAKVCATEAIDP
                     ETKTIDLGRCVFCGLCEEACGGEGIRFSRDPRLPYGKREHLVLGGQEFPTLTASPDLR
                     SLLGRSLHLREVSAGGCNGCDLEAQALGNPIFDLQRFGIDFVASPRHADGLLVTGPVS
                     KNMREALVKTYEAIAEPRIVIAIGACAASGGIFRGSYAVEDGVGNILPVDLFIPGCPP
                     HPLTLLYAILGFLGRSPKE"
     gene            complement(987..>1574)
                     /locus_tag="VF357_01075"
     CDS             complement(987..>1574)
                     /locus_tag="VF357_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522767.1"
                     /translation="LMAELGRLRAEVENVGTTMLDASSAVARMEGTGRLLPRTAKTLQ
                     IVGMAARASGRDVDARRDFPSPAYVESPPAKVLLDNGDVMARATVRFMEMRASFDWLS
                     GILAVAVRPGTIAAEGVVPAAARRLVVSVEEGWRGEIVHAAIVDDESRITRYKVVDPS
                     FRNWPGLSFAVQKNVISDFPVCNKSFNLSYSGADL"
BASE COUNT          289 a          524 c          508 g          253 t
ORIGIN      
        1 tagttgaatg tatagtagac agcaaggaat aggcgataac aggacgtaca acgaaattgg
       61 gggaaagacc gcaaacccaa gcaagacgca acaatggggg ccaacggtta aaaccgctga
      121 ccccctcttg gttccatggc attcaggagg ccgacggttc gatcccgttc agctccacca
      181 gaaaaatcaa gggcctgggt cttcggatcc aggcccttat tcttattctt taggggagcg
      241 gccgaggaaa cccagaatcg cgtacagcag ggtgagagga tggggcgggc accccgggat
      301 gaacaggtcg accggcagaa tgtttcccac gccgtcttcg acggcgtagg agccgcggaa
      361 gatcccgccg ctggcggcgc acgcgccgat cgcgatgacg atccggggct ccgcgatcgc
      421 ctcgtacgtc ttgaccagcg cctcccgcat gttcttcgac acggggccgg tgaccagtag
      481 cccgtccgcg tggcgcggtg aggcgacgaa atcgatcccg aaccgttgaa gatcgaagat
      541 cgggttgccg agcgcctgcg cctcgaggtc gcacccgttg catcccccgg cggacacctc
      601 ccgcaggtgg agcgaccgtc cgagaaggga ccggaggtcc ggggaggccg tcagtgtggg
      661 gaattcctga cctccaagca ccaggtgctc ccgcttgccg tacggcagac ggggatcccg
      721 cgagaaccgg atcccctcgc cgccgcacgc ctcctcgcac aggccgcaga agacgcaccg
      781 cccgaggtcg atcgtcttcg tctccgggtc gatcgcctcc gtcgcgcaca cctttgcgca
      841 cgtttcggcg acgttgccgg accggaacgc ctccacgatc accggcatgc cgcggaacgg
      901 cgccgcgagg tctcccgccg gcagggcgac ggtcactttt cccgtgctcg ctcgcttgcg
      961 tatcgattcc agcatgggac tcctctttac aggtccgcgc ccgagtacga caggttgaag
     1021 ctcttgttgc acacggggaa gtcggagatg acgtttttct ggacggcgaa cgacagtccc
     1081 ggccagttgc ggaacgacgg gtccacgacc ttgtaacgcg tgatccgact ctcgtcatcg
     1141 acgatggccg cgtggacgat ctctccgcgc cacccctcct cgacggagac gaccaggcgg
     1201 cgggcggcgg cggggacaac cccttccgcc gcgatcgtgc ccggccgcac cgcaacggcg
     1261 aggatgccgg acagccaatc gaacgaggcg cgcatctcca tgaatcggac ggttgcgcgg
     1321 gccatcacgt caccgttatc gagcaggact tttgccggtg gagattccac gtaggccggg
     1381 gaggggaagt cccgccgcgc gtcgacgtcc cggccggagg cgcgggccgc catgccgacg
     1441 atttggagcg tcttcgcggt tcgcgggagg agacgccccg tcccttccat ccgggcgacg
     1501 gccgacgagg catccagcat cgtcgtcccg acgttctcca cttcggcccg caggcgcccg
     1561 agttccgcca tcag
//
LOCUS       DASVJL010000060         4153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1321440, whole genome shotgun sequence.
ACCESSION   DASVJL010000060 DASVJL010000000
VERSION     DASVJL010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321440"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..585)
                     /locus_tag="VF357_01080"
     CDS             complement(283..585)
                     /locus_tag="VF357_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX7522768.1"
                     /translation="MTRPDPPGEPPPHCVPVEESIDLHAFSPRDVVSVVEEYLEAAAR
                     RRYREVRLIHGKGTGAQRASVRALLARHPLVESYSDAPPEVGGWGATRVTLKPGPK"
     gene            661..2583
                     /gene="selB"
                     /locus_tag="VF357_01085"
     CDS             661..2583
                     /gene="selB"
                     /locus_tag="VF357_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX7522769.1"
                     /translation="MSKTVILGTAGHIDHGKSSLVRALTGTDPDRLKEEKERGITIEL
                     GFARLSLPSGTLAGIVDVPGHERFVRTMVAGAAGVDIVLLVVAADEGVMPQTREHLDI
                     CRLLAVRHGIVVLNKCDKAESEWMDLQEEEIHALVRGTFLEGAPILRVSAATGEGLPE
                     LVSALDRIATGIPGKDSSDLFRLPVDRSFSMKGFGTVVTGTVIGGTVATGEEVAILPG
                     GTITKVRGLQVHGGPVERSSAGTRTAVNLQGVEKESAPRGSVLCRPGTFSPTKSAEVF
                     VEYLPLVPKPLRNRGQISFHAGTFSCVGRILLYGQAEIPPGGSGYGRVLLSEETILSG
                     GDRFILRGFSPLANFGYTVGGGAVLHPKPPARRGAGKAVPEALPRLRSEDPAKRVLAT
                     VEDAGSAGLTPADAAVVAGLGAERTHGILKGLAAKGIITEVHGSSKIWHRSAVSAASS
                     FCGQALTKLHDRNPERGGFPREEIASLFPVPPDPGFLSLALEGNASISRQGELHFLPA
                     RKPKAVELGSPLARKVAEFLRAAGDTAPGRAELLEAVKGVSADPKAVEKVVDGLARSG
                     EIVRVKELLFDGAALRGIQEKLIAFLAKRGEVTVPEFKEMTGLSRKYIIPLLEHFDAT
                     KITLRVGDKRVLRKGR"
     gene            complement(2702..>4153)
                     /locus_tag="VF357_01090"
     CDS             complement(2702..>4153)
                     /locus_tag="VF357_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX7522770.1"
                     /translation="PGQAEYYYPNQQSARLMWYHDHALGTTRLNAYAGLASAYIIRDP
                     IEQALIKNNVIPSREIPLVLQDKTFVDGTDPNYTRGVAGDLWYPYLYESDRWDVGPGG
                     LPLPVPSVVPEFFSDTIVVNGAVYPYVQVEPRHYRFRILNGSQARFYNLQLYYADSSG
                     KEANLSKSGPSMIQIGTEGGFLPFPVALKNRQIGFDPVTGNANRYTLLLAPGERADVI
                     VDFSSCTSGSKLILYSDAPAPFPMGDPLNDYTTVGTSPNTRTLLQFQVVPRVGAKDPP
                     ALSLLEQLAVNGAAGLGAVAGALLPAIEKFDRRQAARVRDLTLNEDFDESGRLIQMLG
                     TNVLPAPGNPGLFGREYVAAPTETPRQGDTEVWRIFNLTGDTHPIHFHLVNVQVLSRR
                     PFDVGNYNGTPVFTGPARSPDANELGWKETVRMNPGEMTEVAMKFDLPKGKFMNNLPL
                     STRTGITGHEYVWHCHILEHEEHDMMRPLVVKP"
BASE COUNT          681 a         1365 c         1398 g          709 t
ORIGIN      
        1 cgtcccgccg ggggagcagc ttccccggtc gcaaccggtc gcggctccga tggccaggga
       61 tggccaagtg ccgcggaagc catggatggt gggagcggcc ccgtctccgc ccagcgaacg
      121 tgccctgcgg tcacctccca gtgttctgac gacatgcatc ggtcatagaa acatcctctc
      181 ctctatatgc acgtggtctc cgccggttcc gcacgagcga ccctccctcc gggaaccccc
      241 ctgcgtcgtg cgctttgccg gggcgggcgg gggcatgcgc cattacttcg gtcccggttt
      301 gagcgtcacc cgcgtcgccc cccacccgcc gacttcgggc ggagcgtccg aatagctctc
      361 gaccagcgga tggcgcgcga ggagcgcgcg aaccgacgcc cgctgggctc ccgttccctt
      421 cccgtggatc agccgcacct cccggtaccg tctccgcgcc gccgcttcga gatactcttc
      481 cacgacggaa accacgtccc gcggcgagaa ggcgtggaga tcgatgctct cctccaccgg
      541 cacgcagtgc ggaggcggtt ccccgggggg atccggccgc gtcacgaccg gtctttccgg
      601 gagtcgtcgc ccatgttcgg catagtaccg caaccgttcg attacgataa gatatctctc
      661 atgtcgaaaa ccgtgatcct cgggacggcc gggcacatcg accacggaaa gagttccctc
      721 gtgcgcgcgc tgaccggcac cgatcccgac cggctgaagg aggagaagga gcgggggatc
      781 acgatcgaac tcgggttcgc ccgcctctcc ctcccgtcgg gaacgctcgc cggcatcgtg
      841 gacgtgccgg gacacgagcg gttcgtccgg acaatggtgg ccggagcggc gggggtcgac
      901 atcgtcctgc tcgtcgtcgc tgcggacgag ggtgtgatgc cgcaaacgcg cgagcatctc
      961 gacatctgcc gccttctcgc ggtgcgccac gggatcgtcg tcctcaacaa gtgcgacaag
     1021 gcggagagcg agtggatgga tctccaggag gaggagatcc acgcactcgt ccggggaacg
     1081 ttcctcgagg gcgcgccgat cctccgggtt tccgccgcca ccggcgaggg gctccccgaa
     1141 ctggtttccg ccctcgaccg gatcgccacc gggatcccgg ggaaggactc ctcggacctg
     1201 ttccgcctcc ccgtggaccg ctccttctcg atgaaggggt tcggcaccgt ggtcactgga
     1261 accgtgatcg gcggaacggt cgcaacgggc gaggaggttg cgatcctccc cggaggaacg
     1321 atcacgaagg tgcgagggct gcaggtccac ggcggtccgg tggaacgatc gtccgcggga
     1381 acccgcaccg cggtcaacct gcagggggtg gagaaggaga gcgccccgcg tggatcggtc
     1441 ctttgccgtc ccgggacgtt ctcccccacg aagtccgcgg aagttttcgt cgagtacctt
     1501 cctctggtcc ccaagccgct gcgcaaccgg ggccagatct ccttccacgc ggggaccttc
     1561 tcctgcgtcg ggaggatcct cctctacggg caggccgaaa tccctccggg aggctcgggg
     1621 tatggccgtg tgctcctctc cgaggagacg atcctgtcgg gcggcgaccg gttcatcctg
     1681 cgggggttct ccccgctggc gaatttcggc tacaccgtcg gtggcggagc cgtgctgcat
     1741 ccgaaacccc ccgcccgaag gggggccggg aaggcggtcc cggaggctct cccccggttg
     1801 cgctcggaag accccgcgaa gcgggtcctg gccacggtgg aggatgccgg atcggcaggc
     1861 ctcactcccg cggacgctgc ggtcgtcgcc ggtcttgggg cggagcggac ccacggaata
     1921 ctgaaagggc tcgccgccaa ggggataatc accgaagtcc acggatcttc aaaaatatgg
     1981 caccgttcag cggtttccgc ggcgtcgtcc ttctgcggcc aggccctgac gaagctgcac
     2041 gaccgcaacc cggaacgggg cgggttcccg cgggaagaga tcgcctccct gtttcccgtc
     2101 ccccccgatc cggggttcct ctccctcgcc ctggagggaa acgcctccat ctcccgccag
     2161 ggagagctcc acttccttcc ggcccgcaag ccgaaagcgg tggaactggg gagcccgctg
     2221 gcccgcaagg tcgcggagtt cctccgcgcg gcgggcgaca ccgcgccggg ccgcgccgag
     2281 ctcctcgagg cggtcaaagg cgtttcggcg gacccgaagg cggtggagaa ggtggtcgac
     2341 ggcctcgccc gctccgggga gatcgtccgc gtcaaggagc tgctcttcga cggggcggcg
     2401 ctgcggggga tccaggagaa gctcatcgcg tttctcgcga agcgcggcga ggtcaccgtg
     2461 ccggagttca aggagatgac ggggctgtcc cggaaataca tcatcccgct cctcgagcac
     2521 ttcgacgcga cgaaaatcac cctgcgggtg ggggacaagc gggtgctgcg gaaaggcagg
     2581 tagcccgccg tcggacgggg cgtcggcaga ggcgtatgtg ccccgacgcc gaagcgccgg
     2641 ggcacatggg acagcatcga taccttccgc gtccttgaga ccggacggag gcgcggtgcg
     2701 tttacggctt cacgaccaac ggccgcatca tgtcgtgctc ttcgtgctcc aggatgtggc
     2761 agtgccacac gtactcgtgc ccggtgatac cggttctggt gcttaacggc aagttgttca
     2821 tgaacttccc cttgggaagg tcaaatttca tggcaacctc ggtcatttcg ccggggttca
     2881 tccggacggt ttccttccaa ccaagctcat tcgcgtccgg ggaacgggcg gggcccgtga
     2941 acaccggggt cccgttgtaa ttccccacgt cgaagggccg gcgagagagt acctggacat
     3001 tgaccaggtg gaaatggatc ggatgggtgt cgccggtcag gttgaagatg cgccagacct
     3061 cggtatcccc ttgcctcggt gtttccgtcg gggccgccac gtattcgcgt ccaaataaac
     3121 ccgggttgcc gggggcaggc aaaacgttgg tccccagcat ctggatcagg cggccggact
     3181 cgtcgaagtc ctcattcaac gtcaggtccc ggactcttgc cgcctgcctg cggtcgaact
     3241 tctcgatcgc agggaggagg gcgccggcca cggcaccaag accggccgcg ccgttcacgg
     3301 ccagctgctc caggagagaa agggccggag gatccttcgc gccgacgcgc gggaccacct
     3361 ggaactgcag gagcgtccgg gtgttcggac tggtccccac cgtcgtatag tcgttgagcg
     3421 ggtcccccat agggaacggt gcgggggcat cgctgtacag gatgagcttc gaacccgacg
     3481 tgcaactgga gaagtctacg atgacgtccg cccgctcccc cggggccagg aggagcgtgt
     3541 accggttggc gttccccgta accgggtcga agccgatctg ccggtttttc agcgccaccg
     3601 ggaacgggag gaaccccccc tccgttccga tctggatcat gctcgggccg gacttcgaga
     3661 gatttgcctc tttgccggag ctgtcggcat aatagagttg caggttgtag aaccgggcct
     3721 gggaaccgtt caggatccgg aagcggtaat gcctgggctc cacttggaca taaggataga
     3781 ccgccccgtt gaccacgatc gtgtcgctga agaattccgg gaccaccgac ggaactggaa
     3841 gtggcaggcc gcccgggccg acatcccacc ggtcggactc gtacaggtac gggtaccaca
     3901 ggtcacccgc gacccccctg gtgtagttgg gatccgtgcc gtcgacgaac gtcttgtcct
     3961 ggaggaccag gggtatttcg cgggaaggaa tgacgttgtt cttgatgagc gcctgctcga
     4021 tcggatcgcg aatgatgtac gcggacgcca gccccgcgta ggcgttcagg cgcgtggtcc
     4081 ccagcgcgtg gtcgtggtac cacatcagcc gggcgctctg ctggttggga tagtagtact
     4141 ccgcctgtcc cgg
//
LOCUS       DASVJL010000061         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_758941, whole genome shotgun sequence.
ACCESSION   DASVJL010000061 DASVJL010000000
VERSION     DASVJL010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758941"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="VF357_01095"
     CDS             complement(<1..1444)
                     /locus_tag="VF357_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7522771.1"
                     /translation="MFPEIRKVAVLGAGVMGSGIAAHLANAGISCLMLDIVPPTLSDD
                     EKKKGLTEKSPAFRNRFALKGLEGIRKSRPSLIYTQKDLTRIAIGNFDDDFAKIAECD
                     WIVEVVVENLAIKRALYAKIEALWKPGIVVSSNTSGISIAQMMEGRGKEFRRHFLVTH
                     FFNPVRYMKLLEMVSGKDTDPAIFAGMAAFGERVLGKGIVYGKDTPNFVGNRVGVFAM
                     MHAMHVMIEDGLTIEEVDKVLGPAMGRPKSAAFGTADLVGLDTLLHVSDNVYANLKDD
                     PDRERFLPPPFVKEMAKRGLLGRKTGAGFFKMEGKGDTKRKYVLDYNALEYRPAEKVS
                     FPSLDAAKGEEETGARIRKVISGDDKAAKYAWKVLSDTLLYAAKRIPEIAADVVNVDN
                     AMKWGFNWSLGPFETWDAIGLSDSVARMKAEGKAIPANIKKMLSSGASSFYRRRDGAP
                     EFFDFASGGYLPVPVSPDIVYLPSLAERKKV"
     gene            1493..>2250
                     /locus_tag="VF357_01100"
     CDS             1493..>2250
                     /locus_tag="VF357_01100"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HEX7522772.1"
                     /translation="MMIHFRGRVKVWENREMRGIYVHIPFCVRKCGYCDFYSIAGGEE
                     ERDGFPALVEREMDLLLRKFPGEASVPTDTVYFGGGTPTVLGPDRLNRLLAAIRSRFP
                     AAEDAEITTEANPGTVTGDDFARLREGGFNRVSLGVQSFRPETLAALGRIHSVEDIRA
                     AYRDARAAGFSSVGIDLIFGNPGQDEAEWQEDLDLAVTFLPDHVSAYALTPEHGTSLH
                     AAIAGGEIALPDDDTVARMYSAARETLRAAGYRH"
BASE COUNT          377 a          756 c          719 g          398 t
ORIGIN      
        1 ccaccttctt ccgctccgca agggacggga ggtaaacgat atcgggcgag acgggaaccg
       61 ggagatatcc gccggaggcg aaatcgaaaa actccggcgc accgtcccgg cggcgataga
      121 aggaggacgc ccccgaggag agcatcttct tgatgttcgc cgggatcgcc ttcccctcgg
      181 ccttcatcct ggccaccgag tcggacaacc cgatcgcgtc ccacgtctcg aaggggccga
      241 ggctccagtt gaatccccac ttcatcgcgt tgtcgacgtt gaccacgtcg gcggcgatct
      301 ccgggatccg cttcgcagcg tagagaagcg tatcggaaag caccttccag gcgtacttcg
      361 ccgccttgtc gtccccggag atcactttcc ggatccgcgc ccccgtctcc tcctcgccct
      421 tcgccgcgtc gagggacggg aaggacacct tctccgccgg gcggtactcc agcgcgttgt
      481 agtcgaggac gtactttcgc ttcgtgtctc ccttcccctc catcttgaag aagccggcgc
      541 cggtcttgcg gcccagcagg ccccgcttcg ccatctcctt cacgaagggg ggcggaagga
      601 accgctcgcg gtcgggatcg tccttgagat tcgcgtacac gttgtcggag acgtggagga
      661 gcgtgtcgag gcccacgagg tcggcggtac cgaaggcggc cgatttgggg cgccccatcg
      721 cggggccaag gaccttgtcc acctcctcga tggtcagccc gtcctcgatc atcacgtgca
      781 tcgcgtgcat catcgcgaag acgccgaccc ggttcccgac gaaattcgga gtgtccttcc
      841 cgtagacgat ccccttcccg aggacccgtt cgccgaaggc ggccatcccc gcgaagatcg
      901 cggggtcggt gtctttcccg gaaaccatct cgagcaactt catgtaccgg acggggttga
      961 agaagtgggt gacgaggaag tggcggcgga actccttccc cctcccttcc atcatctgcg
     1021 cgatcgagat ccccgaggtg ttcgaggaga cgacgatccc cggcttccag agcgcctcga
     1081 tcttcgcgta gagcgcccgc ttgatcgcca ggttctcgac caccacctcg acgatccagt
     1141 cgcactccgc gatcttcgcg aaatcgtcgt cgaaatttcc gatcgcgatc cgcgtcagat
     1201 ccttctgcgt atagatcagg gaaggtcggc tcttccggat cccctccagc cctttcaggg
     1261 cgaagcggtt ccggaatgca gggctcttct ccgtcaaccc cttcttcttc tcgtcgtcgg
     1321 agagggtcgg cgggacgatg tcgagcatca ggcaggagat cccggcgttg gccaggtgcg
     1381 ccgcgatccc ggaccccatc acgccggccc cgagcacggc caccttgcgg atttcaggaa
     1441 acatcggtcc ctacctcctg ttgggtcggt gtcggacgga acgcgagact gaatgatgat
     1501 tcacttcagg ggacgtgtca aggtgtggga gaatcgggag atgcggggga tctatgttca
     1561 cataccgttc tgcgtccgca agtgcgggta ttgcgacttc tactccatcg ccggcggaga
     1621 ggaggagcgc gacgggttcc ccgcgctcgt ggagcgggag atggacctcc tcctgcggaa
     1681 gttccccggc gaagcgagcg tcccgacgga caccgtgtac ttcggcgggg gaacgccgac
     1741 ggtcctcggg ccggaccggc tcaaccggct cctcgccgcg atccggtcgc gctttcccgc
     1801 ggcggaagac gcagagatca cgacggaggc gaacccggga acggtgacgg gcgacgattt
     1861 cgcccgactg cgggagggcg gcttcaaccg cgtgagcctc ggggtccagt cgttccggcc
     1921 ggaaaccctc gccgccctcg gccggatcca ctccgtggag gacatccgcg cggcgtaccg
     1981 ggacgcgcgt gcggcggggt tctcctccgt ggggatcgac ctcattttcg ggaaccccgg
     2041 ccaggacgag gcggaatggc aagaggatct cgacctcgcc gtgacgttcc tgcccgacca
     2101 cgtgtcggcc tacgcgttga cgccggaaca cggcacgtcg ctccacgcgg cgatcgccgg
     2161 cggcgagatc gccctgcccg acgacgacac cgtcgcccgg atgtactcgg cggcgcggga
     2221 gaccttgagg gccgcgggat accgccacta
//
LOCUS       DASVJL010000062         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_383423, whole genome shotgun sequence.
ACCESSION   DASVJL010000062 DASVJL010000000
VERSION     DASVJL010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383423"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VF357_01105"
     CDS             <1..379
                     /locus_tag="VF357_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522773.1"
                     /translation="ASGVFPSKPAVAVLIPATVELAGIRSFPYGNFVPYLGFGVGFMN
                     VEADTGDVSRRDVNRLCLSMAAGARFYATRWIGARIDVRGRATYLGVRSLGEDRGTFD
                     QGRWFTNAELFGGIFLSFGGKRP"
     gene            383..1534
                     /locus_tag="VF357_01110"
     CDS             383..1534
                     /locus_tag="VF357_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522774.1"
                     /translation="MDPVLALGAFGSVCLLCGNLQTPLVESSAIHLGGTFVYSTVTRK
                     VDTSSGVPPAIAFPGENADTTPHGAAISLSWQTPAPKGLGVGTPVFEVTGGVVEAISH
                     TESNSTGPGGQVAVNASGNGRFESFYGVVRIPLGVRQSLEVGLEVPFNRSRDLIVSGV
                     GNFYSDAGRRDLYSFTTNVALGYRFRGRDWEASAAGLCSTTSTQNGTGYAYSNGSGTL
                     WGGEAQILRRFGKLGTSLAAGAQAGHLTFTEGLYPDFVETRYESPLKRLFMRAEVAYP
                     ISTVSLRFSALWLHVEAPYWDAGATLNVETLLHDGGLDFSARSNEVLLRLTAEIPLRK
                     TVMLQLTALVRQGSETVDFTATSVNPGSASLSLTRTGWAAVGGFRAAVP"
BASE COUNT          331 a          675 c          755 g          355 t
ORIGIN      
        1 ggcgtcgggc gtcttccctt cgaaacccgc ggtcgcggtc ctcatcccgg cgactgttga
       61 gctggccggc atccggagct tcccgtacgg aaatttcgtc ccgtacctcg gcttcggcgt
      121 cggtttcatg aacgtggagg ccgacaccgg ggacgtctcc cggcgcgacg tgaaccgcct
      181 ctgtctctcg atggccgccg gggcgcgctt ctacgccacc cgctggatcg gcgcccggat
      241 cgacgtgcgg ggccgcgcca cctacctcgg cgtgcggagc ctcggcgagg accggggaac
      301 gttcgaccag ggccgctggt tcacgaacgc ggagctattc gggggcatct tcctgagctt
      361 cggcgggaag aggccgtaga aaatggatcc cgtactcgca ctgggagcgt tcggctccgt
      421 ttgcctcctc tgtgggaacc tccaaacacc gctcgtcgag tcctcggcga tccaccttgg
      481 gggaaccttc gtctattcga cggtcacccg gaaagtcgac acatcttcgg gcgtgccgcc
      541 ggccatcgcc ttccccggcg agaacgccga cacgaccccc cacggagctg caatctcgct
      601 ctcgtggcag acgccggccc cgaagggact cggcgtcggc acgcccgtct tcgaggtgac
      661 cggtggcgtc gtcgaggcca tctcgcacac cgagagcaac tcgacgggtc cgggcgggca
      721 ggtcgccgtc aacgcgtccg gtaacggccg tttcgaatcg ttctacgggg tcgtccggat
      781 ccccctcggt gtccgtcagt cgctcgaggt ggggctggag gtgccgttca accgctcgag
      841 ggacctgatc gtctccggag tcgggaactt ctactccgat gctgggcggc gggatctcta
      901 ctccttcacg acgaacgtgg cgctcggata ccggttccgg gggagggact gggaggcgtc
      961 cgccgcgggc ctctgctcga cgacctcgac ccagaacggg acgggctacg cgtactcgaa
     1021 cgggagcgga acgctctggg gcggcgaggc ccagatcctc cggcgattcg ggaagctggg
     1081 gacatccctg gccgcggggg cccaggcggg acacctgacg tttaccgagg gcctctaccc
     1141 ggatttcgtg gagacgcggt acgagtctcc gctgaagcga ctgttcatga gggcggaggt
     1201 tgcctatccc atctcgacgg tctctctccg cttctcggca ctctggctcc atgtcgaggc
     1261 tccatattgg gacgcggggg cgacgctcaa cgttgagacg ctgctccacg atgggggact
     1321 cgacttctcc gccaggtcga acgaggtgtt gctgcggctg acggcggaga tcccgctccg
     1381 gaagaccgtg atgctccagc tgacggcgct ggtgcggcag ggcagcgaga cggtggattt
     1441 cactgcgaca tcggtgaacc ccgggtcggc ctcgctttcc ctcacacgga cgggttgggc
     1501 ggcggtcgga ggcttccgag ccgcggtccc gtgaggaatc cgagcagaga cttgcccgct
     1561 tagtccggaa aggtgctcgc gtctcagcgg ggatccgtgg atccggtgcc tctctgtcga
     1621 tccgaggtcc tgcgaagcca gatgggtgca tcttcggtcg gttcgtagtg ccgctcgaaa
     1681 gacatcgaga aaggaaaaag gaaggataaa ggataaagga aaggataagg aaaggagagg
     1741 aactttgatt cgcgcccgca agggggcgag gcttccgagg ggtcgcggaa gggccgtggg
     1801 gcggccgccg cagcggcgcc gcccggccgc aggctcacgg atcgccccgg tgcggtctgc
     1861 gggcgtcatt cgagcaccgt caggcggatc tctgcgcgtc gggccatagc cggagacatg
     1921 cctccgccct gtcgcctgcg ccggggcgct ggcgtcagcc ccggcagtca ggcgggatca
     1981 gggtgaaacg ggaaccgcgg cggctgcggg aggcggagcg cggtcctggg tcgcgcggcg
     2041 agccgaggcg ccaaggtggt cgaggatgcg gcggatgacg cggccctcgg tgatgaaggc
     2101 gaccacccgc atcacg
//
LOCUS       DASVJL010000063         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1791861, whole genome shotgun sequence.
ACCESSION   DASVJL010000063 DASVJL010000000
VERSION     DASVJL010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791861"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF357_01115"
     CDS             <1..216
                     /locus_tag="VF357_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522775.1"
                     /translation="VRFTMKEDIKDNKIRVTFENLTLLLPASKYSAASENLILSQSYI
                     DPIRPKLLGMADEMAQYVQSSKDKDNW"
     gene            complement(449..535)
                     /locus_tag="VF357_01120"
     tRNA            complement(449..535)
                     /locus_tag="VF357_01120"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(499..501),aa:Leu,seq:cag)
     gene            682..>1660
                     /locus_tag="VF357_01125"
     CDS             682..>1660
                     /locus_tag="VF357_01125"
                     /inference="COORDINATES: protein motif:HMM:NF036509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX7522776.1"
                     /translation="MRNQAFWERWLRQRPKTLEEPRASVKDWYREAGVEKGERRAFKA
                     ALKALNPDGVKGKREKKPPRRGKGRLSGEGTPYGAPQDRRGADGGRTVEGRLRFTREG
                     RPIVIPADPETPIVRIPGHSLSGAWPKDRVLVRLERRRAGGLSYGRVERILERGIRTF
                     VGRFAPTGNRFFVRFRDRESDLLLEVDLPPDFSAEPGDLVLAEVTEYPKGEKEGRARV
                     VHALGKSHTMETLFLAVTSAMDLPVTFPGPVLEEAAAVPQTVRLSARGGGVCHGDGAH
                     PRVDQRDLPFVTIDGEDARDFDDAVCIVREGDGFRLFVAIADVAHYVEPG"
BASE COUNT          330 a          489 c          567 g          274 t
ORIGIN      
        1 gtccgcttca ccatgaagga ggatataaaa gacaacaaga tacgtgttac ctttgaaaac
       61 ctgaccctgc tcctcccagc ctcaaaatac agcgcggcat cggagaattt gatcttgagc
      121 cagagttaca ttgatcccat ccggcctaaa ctgctcggta tggcagacga gatggcgcag
      181 tatgttcaat cttcgaagga taaggacaac tggtaggaac cttttatcaa gaagtgacct
      241 ctccccctcg gataggtatg agatgtgttc atgcttttcg ggccgatacg ggggggggtg
      301 ggcacaccgc ttctcgcgga cgctgtgaag cagcaacgtg ggcgccttga cccattggac
      361 gaattttgag acgatccaaa gaaggttccc agcaggagca ggggaccaac cgcttacaca
      421 tcaagtggtt gcccagaggg ggcaggagtg gtgccgaagg ggggatttga acccccacgt
      481 ggttgcccac accagaccct gaatctggcg cgtctgccag ttccgccact tcggcacgca
      541 ttgcgcgaag atagaagagt aacgcaaaat cggccgtcgc ggcaatctga aaaaaaacat
      601 ccagtaaaaa cagtccattc ggaatcgcct ccgctgccgg tatgatggga aaaagatttc
      661 gacgcgaagg agaaaacgcg gatgcgcaac caggcgtttt gggaaaggtg gctccggcaa
      721 cgcccgaaga cgctcgagga gccgcgcgcg tcggtaaagg actggtaccg cgaggctggc
      781 gtggagaagg gcgagcgccg cgccttcaag gccgcgctga aggcgctgaa cccggacggg
      841 gtgaagggga aacgggagaa gaagccgccg cgccggggga agggacggct ctccggtgag
      901 ggcacgccgt acggcgcccc gcaggaccgc cggggagcgg acgggggccg gaccgtcgag
      961 ggacgcctgc gcttcacgcg cgaggggcgg ccgatcgtga ttccggccga cccggagacg
     1021 cccatcgtgc ggatcccggg gcactccctc tccggcgcgt ggcccaagga ccgggtactg
     1081 gtacggctcg aacgccggcg ggccggcggc ctgtcgtatg gacgggtcga gcggatcctc
     1141 gagcggggga tccggacgtt tgtcggccgg ttcgcgccga cggggaaccg cttcttcgtc
     1201 cgattccgcg accgggagtc cgacctgctc ctcgaggtgg acctcccgcc ggatttttcg
     1261 gcggaacccg gggaccttgt cctggcggag gtcaccgaat atcccaaagg ggagaaggag
     1321 gggcgggccc gcgtcgtcca cgccctcggg aagtcgcaca cgatggagac cctcttcctt
     1381 gccgtgacct ccgcgatgga tctccccgtc acgtttcccg gtccggtgct ggaggaggcg
     1441 gcagccgtcc cgcagacggt ccgtctctcc gcccgcggcg gcggagtgtg ccatggggac
     1501 ggagcgcatc cgcgggtcga ccagagggat ctcccgttcg tcacgatcga cggggaggat
     1561 gccagggact tcgacgatgc cgtctgcatc gtccgggagg gagacggttt ccgcctgttc
     1621 gtcgcgatcg ccgatgtcgc ccattacgtc gagccggggt
//
LOCUS       DASVJL010000064         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1791893, whole genome shotgun sequence.
ACCESSION   DASVJL010000064 DASVJL010000000
VERSION     DASVJL010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791893"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VF357_01130"
     CDS             <1..196
                     /locus_tag="VF357_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522777.1"
                     /translation="RWVTYHGFALNVTTDLSWFRRIHLCGLKGREATSIAEETGNAPT
                     MEAVREAVAASCNRHLEGFA"
     gene            193..1041
                     /gene="lipA"
                     /locus_tag="VF357_01135"
     CDS             193..1041
                     /gene="lipA"
                     /locus_tag="VF357_01135"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX7522778.1"
                     /translation="MNPDRAPRPDWLKVRAPSGKRVEEVSGTLARLGLRTVCREARCP
                     NVGECGGAGTATVILLGDVCTRGCAFCNVTAGMPAPPDPSEPERVAKAAAELSWRHVV
                     VTSVTRDDLPDGGAAHFASVVRALRREAPEATVELLVPDFAGNVDALRCVVDAAPDIL
                     AHNVETVPRLYPVVRKGAEYGRSLELLRRAAAMRPSLLLKSGIMLGFGESEGEVVEVF
                     RDLFAAGCRSITVGQYLPPSRDHLPLSEYVSPERFDLFAEAARAVGFDRVLSGPLVRS
                     SYYRAG"
     gene            1095..2345
                     /locus_tag="VF357_01140"
     CDS             1095..2345
                     /locus_tag="VF357_01140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX7522779.1"
                     /translation="MVVKPKPPIRSILHLDLDAFYASVEVLDRPELRGKPVIVGGDER
                     RGVVAAASYEARKFGVRSAMPTATAKRLCPKGIFLPVRMSRYGEMSDAVFAIYRRFSP
                     LVEPLSIDEAFLDVTGCERLFGGAEEAARKIKAAVREETGLTVSAGVAPNKFLAKIAS
                     DLGKPDGLTVVPPGGEQDFLDPLPVGKLWGVGKVTEEALQGRGIRTIGDLRRSSRETL
                     ARSFGAHGEHLHELARGIDDRPVETEREAKSVGHEDTYDHDLRDRGAMRRELLSLADR
                     VSSRLRGGGIKGKTVTLKVKYHDFVQVTRAISLSDPTDDGGTIYRCALDLLLDTEAGA
                     RPVRLLGISVSKLVPAAGASKKDRMEQLGLFGRPRAARPEAPKPVDPGKKEKLNRAVD
                     RIREKFGPGGIVPGALPQEKRRGE"
     gene            complement(2361..>2581)
                     /locus_tag="VF357_01145"
     CDS             complement(2361..>2581)
                     /locus_tag="VF357_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522780.1"
                     /translation="TACKECHDKVRGLHGKGGHPEAACTDCHKPHAWAITGRDTCLAC
                     HSDMKEHNVSKGACVSCHSFREGKPSKK"
BASE COUNT          390 a          843 c          913 g          435 t
ORIGIN      
        1 gaggtgggtc acctaccacg gcttcgccct gaacgtgacg accgatcttt cctggttccg
       61 gcggatccac ctgtgcggcc tcaaggggag ggaagcgacg tcgatcgccg aggagacggg
      121 gaacgctccg acaatggagg cggtacgcga ggcggtggcc gcctcctgca accgtcacct
      181 ggaggggttc gcatgaatcc ggaccgtgcg ccgcgccccg actggctgaa agtccgcgct
      241 ccctcgggga aacgggtgga ggaggtgtcg gggacgctcg ccaggctcgg tctgcggacg
      301 gtgtgccggg aggccaggtg ccccaacgtg ggggagtgcg ggggggcggg gacggcgacc
      361 gtgatcctgt tgggcgacgt gtgcacgcgg ggctgcgcct tctgcaacgt gaccgccggg
      421 atgcccgctc cgcccgatcc ctccgagccg gagcgtgtag cgaaggcggc ggcggagctc
      481 tcctggcggc atgtagtcgt cacctccgtc acccgcgacg acctccccga cggcggggcg
      541 gcccacttcg cctccgtggt ccgcgcgctg cggcgggaag cgccggaggc cacggtcgaa
      601 cttctggttc cggatttcgc cggaaacgtc gacgcgctgc gatgcgtcgt cgacgccgcc
      661 cccgacatcc tcgcccacaa tgtggagacc gtcccgcgcc tctatcctgt ggttcgaaaa
      721 ggcgcggagt atggccgatc cctcgaactt ctccgccgcg ccgccgcgat gcgtccttcc
      781 ctgttgctga aatccgggat catgctcggg ttcggggagt ccgaggggga agtggtggag
      841 gtcttccggg acctgttcgc cgcggggtgc cgctcgatca cggtggggca atacctgccg
      901 ccttcaaggg atcatcttcc gttgtccgaa tacgtctccc cggaacggtt cgacctcttc
      961 gcggaagcgg ctcgcgcggt cggcttcgat cgcgtcctgt ccggccccct cgtccggagc
     1021 tcttactacc gggccgggtg acccctgtct cctttccggc gcggaatttc cacgtacacg
     1081 ctacaatccc cgatatggtc gtcaagccta agcctccgat acgaagcatc ctgcacctgg
     1141 atctcgacgc gttctacgct tcggtggagg tgctggatcg tccggagctc cgtgggaagc
     1201 cggtgatcgt aggcggtgac gagcgccggg gcgtcgtggc ggccgcatcg tacgaggcgc
     1261 gcaaattcgg ggtccgctcg gcgatgccga cggcgacggc gaagcggctc tgcccgaagg
     1321 ggatcttcct gcccgtccgc atgtcccgct acggggagat gtccgacgcg gtcttcgcca
     1381 tctaccgccg attctccccc ctggtcgagc cgctctccat cgacgaggcg ttcctcgacg
     1441 tcacggggtg cgagcggttg ttcggcggcg cggaagaggc ggcgcggaag atcaaggcgg
     1501 cggtgcggga agagacgggg ctgaccgttt ccgccggcgt ggctccgaac aaattcctcg
     1561 cgaagatcgc gtcggatctc ggaaagccgg acggcctcac ggtcgttcct cctggaggag
     1621 agcaggattt cctggaccct cttccggtgg ggaagctgtg gggggtggga aaggtgacgg
     1681 aggaggcgct ccaggggcgg gggatccgga cgatcggaga tcttcgccgc tcgtcgcggg
     1741 agacgctggc gcgatccttc ggtgcgcacg gggagcacct gcacgaactg gcccgcggaa
     1801 tcgacgaccg ccccgtcgag acggaacgcg aggcgaagtc ggtcggccac gaagacacct
     1861 acgaccacga tctgcgcgat cgtggagcga tgcgcaggga gttgctttcg ctcgccgacc
     1921 gggtgtcttc gcgtttgcgc ggcggcggga tcaaggggaa gacggtcacg ctgaaggtga
     1981 agtaccacga ctttgtccag gtgacccggg cgatctccct gtcggacccc accgacgacg
     2041 ggggaacgat ctaccggtgc gcgctcgacc tgttgctcga cacagaagcg ggagcccggc
     2101 ccgtgcggct cctcgggatc tcggtgtcga agcttgtccc cgcggcaggt gcctcgaaga
     2161 aggaccggat ggaacagctc ggcctcttcg gccgcccgcg tgcggcgaga ccggaggcgc
     2221 cgaaacccgt cgaccccggg aaaaaggaaa agctcaaccg cgcggtggat cggatccgcg
     2281 aaaagttcgg ccccgggggg atcgtccccg gcgcccttcc ccaggagaag cgcaggggcg
     2341 aatagccgct cctcctcccg tcacttcttc gacggtttcc cctcccggaa cgagtggcag
     2401 gagacgcacg cccccttcga aacattgtgt tccttcatgt cgctgtggca cgcgaggcac
     2461 gtgtcgcgcc ccgtgatcgc ccaggcgtgc ggtttgtggc aatccgtgca ggcggcctcc
     2521 gggtggcctc ccttgccgtg caggccgcgc accttgtcgt ggcactcctt gcacgcggtg
     2581 a
//
LOCUS       DASVJL010000065         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1322298, whole genome shotgun sequence.
ACCESSION   DASVJL010000065 DASVJL010000000
VERSION     DASVJL010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322298"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..716
                     /gene="pgsA"
                     /locus_tag="VF357_01150"
     CDS             144..716
                     /gene="pgsA"
                     /locus_tag="VF357_01150"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX7522781.1"
                     /translation="MTSDTRLNAPNILTLLRILTIPVVVLFLLPPAGREISFARSVAA
                     FSLFVVATITDLFDGYIARRYHMVTTLGKLLDPVADKLLVCAAMIMLIPPGRVPAWMA
                     VIVVAREIGVTALRGVASSEGVIIAASKLGKAKTLLLNIGVGVLILHYPILGIPVHGV
                     GMVFLSAGLVLTAWSGLDYFFRFVAEIFNR"
     gene            774..848
                     /locus_tag="VF357_01155"
     tRNA            774..848
                     /locus_tag="VF357_01155"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:806..808,aa:Gly,seq:gcc)
     gene            913..2244
                     /locus_tag="VF357_01160"
     CDS             913..2244
                     /locus_tag="VF357_01160"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7522782.1"
                     /translation="MTALSREGRPERIGWYFYDFANSAFSTTVVTVFTGPYLTSVARA
                     AADPAGYIHPLGIPVLAGSFFPYVVSLSVFFQVLLLPLLGAIADYSRRKKPMLFLFAY
                     AGSSATVGLYFLEGTRYLLGGGLFLLANVCFGASVVFYNAWLPEIAPPGDRDSVSSVG
                     WALGYLGGGVLLLLNLILFSRAHDFGLTSGEAVRISLASAGAWWAVFSLVPLATMRRR
                     ETARPLPAGEGFLGTGFRQLRRTFEEAKGHPQLLLFLGAYLLYNDGIQTVIALSSQFG
                     QEELGLSVSTLTSAILMVQFVAFFGALLFNVFAKRVGAKAAVAISLVLWTGTLVYAYA
                     GLRDATGFYAMAACVAVVLGGSQALSRSIFSRMIPVGREAEYFSLYEVSERGTSWLGP
                     LFFGLALQFTGSYRVAILSLAVFFIAGLALLLRVDVARAEAEATPAPSRTP"
BASE COUNT          316 a          779 c          778 g          505 t
ORIGIN      
        1 gaagggaacc tcaccaacac gatcgcggcg tacaacgccg ggccggccgc ggtcagaaaa
       61 tacggcggcg tcccccccta ccaggagacc cgcctctacg ttcgtcgcgt gatgggcctg
      121 taccgggaat attccgccgc cgaatgacca gcgacaccag gctgaacgcg cccaacatac
      181 tgaccttgct gcggatcctg acgatcccgg tggtggtcct cttccttctc cctcccgcgg
      241 gcagggagat ctccttcgcg cgttcggtgg cggcgttctc cctgttcgtc gtcgccacga
      301 tcaccgacct cttcgacggg tacatcgcgc ggcgctacca catggtcacc actcttggga
      361 aactgctcga cccggtggcc gacaagctgc ttgtatgcgc cgcgatgatc atgctcatcc
      421 cgccgggcag ggtcccggcg tggatggcgg tcatcgtggt cgcccgggag atcggtgtca
      481 cggcgctgcg cggggtcgcc tcctcggaag gggtgatcat cgccgcctcg aaattgggga
      541 aggcgaagac cttgctcctg aatatcgggg tcggggtcct catcctccat tacccgatcc
      601 tcgggatccc ggtccacggc gtggggatgg tgtttctgag cgccgggctg gttctcaccg
      661 cgtggtccgg gctcgactac ttcttccgct tcgtggctga aatcttcaat cggtaggttg
      721 gcggcttttc ttgacaagcc gcgcgcggtt cacctacaat cgacgttcgt tacgcgggcg
      781 taactcagtg gtagagtgcg accttgccaa ggtcgaagtc gcgagttcaa atctcgtcgc
      841 ccgctccatc ggatcccaaa aacccccggc gcttcgcgaa gcgaccgggg gtttttcgta
      901 aaggaggtcc cgttgacggc gctttcgcgg gaaggtcgtc ccgagcggat cggttggtac
      961 ttttacgatt tcgcgaactc ggctttctcc acgacggtgg tcaccgtgtt cacggggccg
     1021 tatctgacct ccgtcgcccg cgccgccgcc gaccccgcgg ggtacattca tcctctcggc
     1081 atccccgtgc tcgcggggtc gttcttcccg tacgtcgtct ccctctccgt cttcttccag
     1141 gtactcctct tgccgctgct gggggcgatc gccgactatt cccgacggaa gaaaccgatg
     1201 ctgttcctct tcgcctatgc gggatcctcc gcgacggtgg ggctctattt cctcgaaggg
     1261 acgcggtacc ttctgggtgg ggggctgttc ctgctggcca acgtctgctt cggggcctcg
     1321 gtggtctttt acaacgcctg gctgccggag atcgcgcctc cgggggaccg cgattccgtc
     1381 tcctccgtgg ggtgggcgct cgggtacctc ggaggagggg tcctccttct cctgaacctg
     1441 atcctcttct cccgtgcgca cgacttcggc ctgacgagcg gagaggcggt ccggatcagc
     1501 ctcgcctcgg cgggggcgtg gtgggccgtc ttctccctcg tgcccctcgc cacgatgcgc
     1561 cgccgggaga ccgctcggcc gcttccggcg ggggaggggt tcctcggcac gggattccgg
     1621 caactgcgtc gcacgttcga ggaggcgaag ggccacccgc agctcctgct gttccttggc
     1681 gcctacctgc tttataacga cgggatccag acggtgatcg ccctctcgtc ccagttcggc
     1741 caggaggagc tgggcctctc cgtttcgacg ctgacctcgg cgatcctcat ggtccagttc
     1801 gtcgcttttt tcggggccct gctcttcaac gtgttcgcga agcgggtagg ggcgaaggcg
     1861 gcggtggcga tcagtctggt cctgtggacg gggacgctcg tctacgccta cgcagggctt
     1921 cgggatgcga ccgggttcta cgcgatggcg gcatgcgtgg ccgtcgttct cggcgggagc
     1981 caggcccttt cccgctccat cttctcgcgg atgatcccgg tcgggcggga ggcggagtac
     2041 ttctccctgt acgaggtgag cgagcggggg acgagctggc tggggcccct ctttttcggg
     2101 ctggcgctgc agttcacggg aagctaccgc gtcgcgatcc tctcgctggc ggtcttcttc
     2161 atcgcggggc ttgccctgct gctgcgcgtg gacgtcgcgc gcgccgaggc ggaagctacg
     2221 cctgccccaa gtaggacgcc ttgatccgcg gttcccggag caactctttg gaggggccgg
     2281 agagggcgat cctgcccgtc tcgaggacgt aagtgtaggt ggagaccttc agcgcctgct
     2341 tcacgttctg ctcgacgagc aggatggtgg tcccggcc
//
LOCUS       DASVJL010000066         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1698042, whole genome shotgun sequence.
ACCESSION   DASVJL010000066 DASVJL010000000
VERSION     DASVJL010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698042"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..239
                     /gene="rpmH"
                     /locus_tag="VF357_01165"
     CDS             87..239
                     /gene="rpmH"
                     /locus_tag="VF357_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX7522783.1"
                     /translation="MKRTYQPHNLRRKRTHGFRERMSTPGGRKVISRRRAKGRKRLSV
                     TVSGKK"
     gene            236..646
                     /gene="rnpA"
                     /locus_tag="VF357_01170"
     CDS             236..646
                     /gene="rnpA"
                     /locus_tag="VF357_01170"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX7522784.1"
                     /translation="MTVSPGERPRSFPASERLRTDREFREVVRKGARLHTTHYTVYRD
                     SLGGGKRQVGISAGKRAGGAVARNRAKRLLREFYRMHKGVFPRGTRTAIVVRKPPEGA
                     VLSSVCAELLPALLRRWGGEGDFPCGPDISSSAS"
     gene            613..870
                     /gene="yidD"
                     /locus_tag="VF357_01175"
     CDS             613..870
                     /gene="yidD"
                     /locus_tag="VF357_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX7522785.1"
                     /translation="MRARHLLIGLLRLYQTAVSPSIGNCCRFHPNCSEYMIGSIRLNG
                     AILGALDGIRRIGRCHPFHKGGVDLPRRIQLFGTRAPWKNG"
     gene            855..2519
                     /gene="yidC"
                     /locus_tag="VF357_01180"
     CDS             855..2519
                     /gene="yidC"
                     /locus_tag="VF357_01180"
                     /inference="COORDINATES: protein motif:HMM:NF002353.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX7522786.1"
                     /translation="MEKRMMLAIVLSIAVLLGYQYFYSAPPPPPGVAAGKDNAAVVPA
                     PAAKGGPIPAPAAVAGGLAAKTAASGRTITVKTPLYSAALTTEGGGVSSFLLSEYKDT
                     PGPGGKPLDILGGKPPLPPTLSLYLDEDRPPLPSPLTFSSDAPAEIAVKAGETRSVLL
                     TWESTSGVRVSREYVFHGDKYEFEARVQATNGSREPVAVRPGLELEQVYEGELAGDSY
                     SFHGIVVGSGKSGLKRYDLKDITKGKVEKGPARWLAADAKYFTWILLPEREWTVTRAS
                     LAGETGVRVAAADTAATLQPGDTVRAGARVFAGPKRSALLEATGKDLPDLIDFGWFAV
                     VAKPLVFLMKASNRVTGNYGIDIILLTVLIKILFYPLTQKSIVSMRKMQELGPILKTL
                     KEKYKGDSQRLNQETMNLYKTYKINPLSGCLPMVAQIPVFIALYKGLLVTIELRHAPF
                     FLWVNDLSAPEHLWDIAVAGYTVPIRLLPLLMGISMFVQQKMTPSAGMEPAQQKMMLF
                     MPLIFTFMFWSFPTGLVIYWLVNNVLAIGQQMIYNRKTEAAKAATA"
     gene            2749..>3593
                     /locus_tag="VF357_01185"
     CDS             2749..>3593
                     /locus_tag="VF357_01185"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="HEX7522787.1"
                     /translation="MTREDTTIVALATPGGLGAISVVRLSGPDAFQVAGHLTGLSPPL
                     APPRTLRRCPVRDASGEILDIALAVFFPGPHSFTGEDVVEIHLHGNPVVVETVVRAAC
                     IAGAVPAEPGEFSRRAFHRGKMDLTQAEGLCDLIAARTGAAARSALRQMRGGLREEIV
                     PLRERLLSLLTLQEAAIDFAEEDDVSAITSGQLAERVSEIMGQLEELLRSYESGRRFR
                     DGATVVIAGVANVGKSRLLNRLAGEERAIVTETPGTTRDYRHADVPIGGVPVTVIDTA
                     GLRETA"
BASE COUNT          610 a         1184 c         1194 g          605 t
ORIGIN      
        1 cggttgacac ggtggtccgt aaggcaatag attacatggt tcccgtcctt tgagcgagtc
       61 gtgttgatgg aaggagcgaa attcccatga agcgcacgta ccagccgcac aacctgcggc
      121 ggaaaagaac ccacggcttc cgcgagcgga tgtcgacccc tggcggccgg aaagtcattt
      181 cacggcgccg cgccaagggg cgcaagcgtc tctccgtaac ggtcagcggc aagaagtgac
      241 cgtctccccc ggggaacgtc cgcggagctt ccccgcgagc gagcgcctgc gcaccgaccg
      301 ggagttccgg gaagtggtgc ggaaaggcgc gcgcctgcac acgacgcatt atacggtgta
      361 ccgcgactct ctgggcggtg gcaagcgcca ggtgggcatc tccgccggga agcgcgcggg
      421 aggcgccgtc gcccgcaacc gcgccaagcg gctgttgcgg gaattctacc ggatgcacaa
      481 aggcgtcttc ccgcggggga cccggaccgc gatcgtggtt cggaagcccc ccgagggggc
      541 ggtcctttcc tccgtgtgcg ccgaactgct ccccgcgctt ctacggcgct ggggaggcga
      601 gggagacttt ccgtgcgggc ccgacatctc ctcatcggcc tcctgaggct gtaccagaca
      661 gcggtttccc cttccatcgg caactgctgc aggttccacc ccaactgctc cgagtacatg
      721 atcggatcga tccgcctgaa cggggcgatc ctcggggcgc tggacgggat ccggcggatc
      781 ggccggtgcc atccctttca caagggaggc gtcgacctgc cccggcggat tcaacttttc
      841 ggaacgaggg caccatggaa aaacggatga tgctggcgat cgtgttgtcg atcgccgtcc
      901 tgctcggata ccagtatttc tactccgctc ccccgcctcc tccgggcgtg gcggcgggaa
      961 aggacaacgc ggcggtcgtt cccgcgccgg cggcaaaggg tgggccgatc cccgcgccgg
     1021 cggccgtcgc cggggggctc gcggcaaaga ccgccgcgtc gggccgaacg atcacggtaa
     1081 agacccccct gtactccgcg gccctcacca ccgaaggcgg cggcgtttcc tccttcctgc
     1141 tgtccgagta caaggatacc cccggtcccg gcgggaagcc gctggacatc ctcggcggga
     1201 agccgccgct cccgccgacg ctgtcgctct acctcgacga ggaccgccct cccctgccct
     1261 cccccctgac cttctcctcc gacgcaccgg ccgagatcgc ggtgaaggcg ggagagaccc
     1321 gaagcgtcct gctcacgtgg gaatcgacct ccggggtccg ggtgtcgcgg gaatacgtgt
     1381 tccacgggga caagtacgag ttcgaggccc gcgtccaggc gaccaacggg tcgagggagc
     1441 cggtcgcggt gcgccccggg cttgagctcg agcaggtcta cgagggggag ctggctggag
     1501 actcctactc cttccacggc atcgtcgtcg gatccggcaa gtccggcctg aagcggtacg
     1561 acctgaagga catcaccaag gggaaagtcg aaaagggacc ggcgcgttgg ctcgcggcgg
     1621 acgcgaaata tttcacctgg atcctcctgc ccgagcggga gtggacggtt acgcgcgcgt
     1681 cgctggccgg ggagaccggg gtccgcgtgg cggcggccga cacggcggcg acgttgcagc
     1741 cgggcgacac ggttcgcgcc ggagcccgcg ttttcgcggg gccgaagcgg tccgcgctgc
     1801 tcgaggccac ggggaaggat cttccggatc tgatcgactt cggatggttc gccgtcgtcg
     1861 cgaaaccgct cgtcttcctg atgaaggcga gcaacagggt gacggggaac tacggcatcg
     1921 acatcatcct gctcaccgtc ctgatcaaga tcctcttcta ccccctcacc cagaagtcga
     1981 tcgtctccat gcggaagatg caggagctgg ggccgatcct caagacgctg aaggagaagt
     2041 acaaagggga ctcgcagcgc ctcaaccagg agacgatgaa cctgtacaag acgtacaaga
     2101 tcaatcccct ctccggatgc ctgccgatgg tggcgcagat cccggtgttc atcgcccttt
     2161 acaaggggtt gctggtcacg atcgagctgc ggcacgcgcc gttcttcctc tgggtgaacg
     2221 acctgtccgc ccccgagcac ctctgggaca tcgccgtggc ggggtacacc gttccgatcc
     2281 gcctgctgcc gctcctgatg gggatctcga tgttcgtcca gcagaagatg acgccgtcgg
     2341 cggggatgga gccggcgcag cagaagatga tgctgttcat gcccctcatc ttcaccttca
     2401 tgttctggag cttcccgacc gggcttgtca tctactggct ggtgaacaac gtcctcgcca
     2461 tcgggcagca gatgatctac aaccgcaaga cggaggcggc aaaggccgca acggcgtaat
     2521 cgtccgattc aaaagtacgg caacttaaat ggagggactt tgatcatcgc gtgatacccc
     2581 cttcctcgct acgtttcggc agccgtccct ggctgccttc actggccgct cctgcgcctc
     2641 catgtgtccg cggcacttga ccatccatgg ccatcggcga gaacacgccc gctcggaggg
     2701 ggtatcccgc tccgatgccc ctccggggat gcagcagcaa ataataagat gacccgcgaa
     2761 gacacgacga tcgtcgcgct cgccacaccc ggggggttgg gcgccatctc cgtcgtgcgc
     2821 ctctccggcc cggacgcttt tcaggtagcc ggccatctca ccggtctctc ccctcctctc
     2881 gctcccccga ggacgttgcg acggtgtccc gtccgcgacg cctccggcga gatcctcgac
     2941 attgcgctcg ccgtcttctt ccccggaccg cacagtttca ccggcgagga tgtcgtggag
     3001 atccatcttc acggaaatcc cgtggtggtg gagaccgtcg tccgtgcggc atgcatcgcg
     3061 ggggccgtgc cggcggaacc gggggagttc tcgcggcgag cgtttcaccg ggggaagatg
     3121 gacctgaccc aggcggaggg cctttgcgat ctcatcgcgg cccggacagg ggctgcggcg
     3181 cgctcggcgc tgcggcagat gcggggcggc ctccgggaag agatcgttcc gctgcgggag
     3241 cggcttctct ccctcctcac actccaggag gcggcgatcg acttcgcgga agaggacgat
     3301 gtctcggcga tcacaagcgg acaacttgca gaacgggttt cagaaataat gggtcaactg
     3361 gaggagctcc tgcggtcgta cgagtcgggg cgccggttcc gggacggcgc gacggtcgtg
     3421 atcgccggtg tcgcgaacgt cgggaaatcg cggctgctca accgcttggc gggggaggag
     3481 cgcgcgatcg tgacggagac gccggggacc acgcgggatt atcgccacgc cgacgttccc
     3541 atcggcggcg tcccggtgac cgtgatcgac acggcggggc ttcgggagac ggc
//
LOCUS       DASVJL010000067         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1041541, whole genome shotgun sequence.
ACCESSION   DASVJL010000067 DASVJL010000000
VERSION     DASVJL010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041541"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VF357_01190"
     CDS             <1..610
                     /locus_tag="VF357_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7522788.1"
                     /translation="ISVDVRIVAATNKDLQSRIRAGSFREDLYYRLNVVEVHVPPLRE
                     RPEDVPALVEHFVGELAEGRDLTVPPRVMEELMRRPWPGNVRELKNACERMVILCDGN
                     EVSLEDLPAPSDRMRPEKAPLLGNGGDLPSLPPEGLSLVDLEKKVIESALRIKNGNMT
                     QTAVFLRIPRHVLIYRLEKYGIRRGGSLADDNRGGAEYSSVD"
     gene            765..1271
                     /locus_tag="VF357_01195"
     CDS             765..1271
                     /locus_tag="VF357_01195"
                     /inference="COORDINATES: protein motif:HMM:NF021587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2318 domain-containing protein"
                     /protein_id="HEX7522789.1"
                     /translation="MAKISGKGMAIMVAVVLLVGVGSLAYSDMFSGLFKKSPAESAKA
                     AGVVETADAVKIPLKALDSGKALLLSLGSDGRQVFYFALKSRDGAYRAALDACDVCFR
                     TNRGYRQEGDQMVCNNCGQKFACDKIGEVKGGCNPHPLARGIEGQYLVIKKADIAAGK
                     EYFARKRS"
     gene            1306..2457
                     /locus_tag="VF357_01200"
     CDS             1306..2457
                     /locus_tag="VF357_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7522790.1"
                     /translation="MNIRTLAWKNLLRRKSRAIFTGLGIFLGIATFVAISSITSQMEG
                     AVQDQLDRFGANIVVSPRTEQVSLGFGDIALGGTEVAHSRLTMADKERIASIHHKDRI
                     RSIVPILMTGVDASGKNLVWMGLPAADVAGARPWWKIQGGTGLKHGEVFLGAEAAALL
                     DKGPGGTVTAGDRLFRVAGVLPPTGEKEDGMVIADIADVQALAKKPGGVTYFEVAALC
                     KDCPVEDILAQIGQALPGARVSAIRQVVESRKAAVDQLRRLGFGVSAIVLLIGGLMVL
                     VTVMSGVQERTREIGVLREVGFRKRSIFSLLFWETGWVSLFSSLLGAGTGIAVAYLAS
                     PVFGIEHPAVVFSPAIFGAVAGLSLGLIGAIPPARRAAALSPTEAIRSL"
     gene            2474..>2993
                     /locus_tag="VF357_01205"
     CDS             2474..>2993
                     /locus_tag="VF357_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7522791.1"
                     /translation="MEPVIHLNDVSMVYGCNGTRTTALAGVSFDIRQGEYVVITGESG
                     AGKSTLLTILGGLQVPSGGTVRIGGADLSALLPDGLADFRRGTIGFVFQAYHLLPYLT
                     ARENVMVPMSPERIHPREKVLLADTLLASVGLAGKEDRLPSQMSGGENQRVAIARALV
                     HDPPVLLADEPTG"
BASE COUNT          526 a          884 c         1048 g          535 t
ORIGIN      
        1 catttccgtg gacgtgcgga tcgtcgccgc gacgaacaag gacctgcaga gccgaatccg
       61 ggcagggagc ttccgggagg acctctacta tcgccttaac gtggtcgagg tccacgtgcc
      121 tcccttgcgg gaacggccgg aagacgttcc ggcgctcgtc gagcactttg tcggggaact
      181 ggcggagggc cgggatctca cggtgccgcc ccgggtcatg gaggaactca tgcgccgccc
      241 ctggcccggc aacgtccggg agctgaagaa cgcctgcgag cggatggtga tcctctgcga
      301 tggcaatgaa gtgtccctgg aggacctccc ggcgccttcc gacaggatgc ggccggaaaa
      361 ggcgcccttg ctcggcaacg gcggggacct gccgtccctt ccgccggagg ggctctcact
      421 ggtggacctg gagaagaagg tgatcgagag cgccttgagg atcaagaacg ggaatatgac
      481 ccagacggcc gtcttcctgc gcatccccag acatgtcctg atctaccggc tcgaaaagta
      541 cgggatccgc cgcggaggct ccctggccga cgataaccgg ggaggcgcgg aatactcttc
      601 agtcgattga agaatatcct tcagcaaccc ctctcgaccc cgtccgttgc tttccgttcc
      661 aacatcaaac tgttcgataa caataaggat taaatagttt gctcccgtgg gcacgggtcg
      721 tgcttggtat tacccgtcaa atacttcccg gagggttgca ccgcatggcg aagatcagtg
      781 gcaaaggaat ggcgatcatg gtggcggtgg tattgcttgt gggagttggc tcgcttgcat
      841 attctgatat gtttagtggc ctcttcaaga aatcgccggc ggaaagcgcg aaagcggccg
      901 gggtggtgga gaccgcggac gccgtgaaga tccccctgaa ggctctcgat tccgggaaag
      961 ctctcctcct ctcgctggga tcggatgggc ggcaggtttt ctatttcgcg ctgaaaagcc
     1021 gggacggggc gtaccgggcg gcgctcgacg cctgcgacgt ctgcttccgg acgaaccgcg
     1081 gataccggca ggaaggtgac cagatggtgt gcaacaactg cgggcagaag tttgcctgcg
     1141 acaagatcgg cgaggtgaaa ggcgggtgca acccgcatcc tctcgcacgg gggatcgagg
     1201 ggcagtacct cgtgataaaa aaggccgaca tcgccgcggg caaggaatat ttcgcccgga
     1261 agcggtcatg agcgctcccc gtacatcccg aacggggagt cgttcatgaa cataagaacg
     1321 cttgcgtgga agaacctgct tcggcggaag tcgcgcgcca tcttcacggg cctcgggatc
     1381 ttcctgggga tcgccacgtt cgtcgcgatc tcgtcgatca cctcgcagat ggaaggggcg
     1441 gtccaggacc agttggaccg gttcggcgcg aacatcgtgg tgtccccgcg aacggagcag
     1501 gtatccctcg ggttcggcga catcgctctc ggaggaacgg aggtggcgca ctcccgcctc
     1561 acgatggcgg acaaggagcg catcgcgtcg atccaccaca aggaccggat ccgttccatc
     1621 gtcccgatcc tgatgaccgg cgtggacgca tccggaaaga acctcgtctg gatgggactt
     1681 ccagcagcgg acgttgcggg ggcccgcccg tggtggaaga tccagggggg aacgggactg
     1741 aagcacggag aggtgtttct gggcgccgaa gccgcggcgc tgctcgacaa gggaccgggg
     1801 ggaaccgtga cggccgggga ccgcctcttc cgcgtcgccg gggtgcttcc gccgacgggg
     1861 gagaaagagg acgggatggt catcgccgac atcgcggacg tgcaagcgct ggcgaaaaaa
     1921 ccggggggcg tgacatattt cgaagtggcg gcgctgtgca aggattgccc ggtggaggac
     1981 atcttggcgc agatcgggca ggcgttgccc ggcgcgcggg tgtcggcaat ccgccaggtg
     2041 gtggagagcc ggaaggccgc ggtggaccag cttcggcgcc tcgggttcgg cgtctctgcg
     2101 atcgtgctgc tgatcggggg gctcatggtg ctggtgaccg tgatgagcgg ggtgcaggag
     2161 cggacgcgcg agatcggtgt cctgcgtgag gtcggattcc ggaaacgctc gatcttctcc
     2221 ctcctgttct gggagaccgg ctgggtgtcc ctcttctcct cgctgctcgg ggcggggacg
     2281 ggaatcgccg tcgcgtacct tgcgtcgccg gtgttcggaa tcgagcatcc tgcggtcgtt
     2341 ttctccccgg cgatcttcgg ggccgtcgcc ggcctttccc tcgggttgat cggtgccatc
     2401 ccgccggctc gccgcgccgc ggccctgtcg cctaccgaag cgatccgcag cctttaatac
     2461 aacaggagaa acgatggaac cggtcattca cctgaacgac gtttcgatgg tctacggatg
     2521 caacggaacc cggacgacgg cgctggccgg ggtctctttc gatatacggc agggggagta
     2581 cgtcgtcatc acgggggagt cgggggcggg gaagagtacg ctgctgacga tcctcggggg
     2641 gcttcaggta ccttcgggag ggacggtccg gatcggcggc gcagacctct ccgcgctcct
     2701 gccggacggt ttggccgact tccggcgggg gacgatcggg ttcgtcttcc aagcgtacca
     2761 cctcctcccg tatctgacgg cgcgggaaaa cgtgatggtg cccatgtcgc cggagcggat
     2821 ccacccccgc gagaaagttt tgctggccga caccctgctc gcctccgtcg ggctggccgg
     2881 gaaggaggac cgcctccctt cgcagatgtc cggcggggag aaccagcggg tggcgatcgc
     2941 gcgggcgctg gtccacgatc cccccgtgtt gctggcggac gagccgacgg gga
//
LOCUS       DASVJL010000068         4853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_197187, whole genome shotgun sequence.
ACCESSION   DASVJL010000068 DASVJL010000000
VERSION     DASVJL010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197187"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VF357_01210"
     CDS             complement(<1..954)
                     /locus_tag="VF357_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7522792.1"
                     /translation="MPIDVGPIRAAEFPVTENFLYLNHAGVSPIPARAAEAGIRMLQL
                     ARDEGAYRLRKWEELANETRDRFARIVGASPDEVAFVKNTSEGLSFVAAGFPWKEGDN
                     LVTANVEYPSNVYPWLRLRTRNVEVRMVPAREGRVRKEDLFAACDGKTRLITLSSVEF
                     LNGYRNDLAGIGEYCQKHGIFFCVDAIQSVGVLPMDVKSFGIDALSADGHKWLLSPEG
                     IGAFYISRDVMEMVEPVILGWHSVKNRFDFENYDFHLSPDARRYEPGSMNTVGISSFN
                     ASLELLLSLGMDRIWERVRRLTERVRERAVEEGCIMVSPDHP"
     gene            1015..1827
                     /locus_tag="VF357_01215"
     CDS             1015..1827
                     /locus_tag="VF357_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEX7522793.1"
                     /translation="MDWSGENTLRYSRNILVEELGEAGQERLFASSALVVGAGGLGSP
                     ALLYLAAAGIGRIGVIDDDRVDITNLNRQVIHRADAVGQWKADSAAAALRAFRPDLAV
                     DVYPEALTAANAAALFRRYDAVVDASDNFSTKYLCNDAAVVSRRPLVHAGVLRFGGQM
                     LTVVPGAGPCLRCVIPRIPSRKDSPGCAEAGIIGAAAGVLGAWQAMEAIKLLSGTAPS
                     PGGKLLTIDTLSNEVSCLTVVRDRSCPACGEHPRITEPLSAAEYDHDRMCAG"
     gene            1824..2864
                     /locus_tag="VF357_01220"
     CDS             1824..2864
                     /locus_tag="VF357_01220"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF014610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEX7522794.1"
                     /translation="MNLPATLSPPRKAVVLLSGGLDSTLAARIVQDQGIELHAIHFTS
                     PFCTCSHNGPGDGRGCRSAAQEVAVGMGIPIRTVSKGEEYLEIVKHPRHGRGSAMNPC
                     IDCRIFTLRKAKAFMEEIGASFLVTGEVVGQRPMSQRDDTLRGIEKHSGCAGIILRPL
                     SALHLPPTLPEREGWVDREKMLSITGRSRKEQIRLAKEFKIGDYPCPAGGCMLTDRTF
                     SIKVRDLLDHHPSFGMHEVLLLKAGRHFRVQGMRAIVAKSEEENRRLEALCRGRDTVY
                     VAHSHPGPSVTLLGGSEEERLDLLSRIFTRYGKAGSVGPYEIREISPDGERLVTVREN
                     PDFEEVGRGLLC"
     gene            2848..3735
                     /gene="larE"
                     /locus_tag="VF357_01225"
     CDS             2848..3735
                     /gene="larE"
                     /locus_tag="VF357_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX7522795.1"
                     /translation="MDYSAETWNGAPTPDDLRRYEALLAALRRMGSAVVAFSGGVDSS
                     FLLHAAKEAMGERVLAVTATSPTYPRSEREEAVRLARSWGVRHHLVESNELEIPGFSA
                     NPPDRCYHCKKELFGILAGIARREGYAAVCDGSNADDVRDFRPGRRAAKELSVRSPLL
                     ENGLGKPAIRRLSRSFDLPTADRGSFACLSSRFPYGTRIDEESLRRVEACEEVLRGFG
                     FRQFRVRVHDAVARIEVGIDEIPRLFQPEISEAVHARFRENGFLYVAVDLKGYRTGSM
                     NEGLPAISPGLVAGPPEDL"
     gene            complement(4136..>4853)
                     /locus_tag="VF357_01230"
     CDS             complement(4136..>4853)
                     /locus_tag="VF357_01230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7522796.1"
                     /translation="GEPSVLVRDASQEPGWQDFVGVARTLACVPLHGDGEILGTVTIF
                     DKFPHKTFFPSSFTTEDLATFGKFLKYAEKAVVNAIAYERNDRLKNLDEATSLPTLKY
                     FQERLLHEISRARRFQRRLVLMICEVQSHVPSGSQSRARRADWMMKQVAKAIRASLRE
                     YDIVARISEVKFGMILPEAEDGKISAIPRIKKAIAAEAEEIQRCARDARVEVRFGHAS
                     FPEDGDDHEKLIFKSNILKN"
BASE COUNT          806 a         1619 c         1628 g          800 t
ORIGIN      
        1 gggatggtcg ggagaaacca tgatgcaccc ctcctccacc gccctttcgc ggacccgttc
       61 cgtgagccgg cgcacgcgct cccagatccg gtccatccca agcgacagca ggagttccag
      121 cgaggcgttg aacgacgata tgccgaccgt gttcatgctt cccggttcgt accgccgggc
      181 gtcgggggaa aggtgaaagt cgtaattctc gaagtcgaac ctgttcttca cggagtgcca
      241 cccgaggatc accggctcga ccatctccat gacatcccgg gagatgtaga aggcgccgat
      301 cccctccggc gacaggagcc acttgtggcc gtccgcggag agggcgtcga tgccgaagga
      361 cttcacgtcc atcgggagaa cgcccaccga ctggatcgcg tctacacaga agaagatccc
      421 gtgcttctgg caatactcac caattcccgc caggtcgttc cggtagccgt tcaggaactc
      481 cacggaagag agagtgatca ggcgggtctt cccatcgcag gcggcgaaca gatcctcctt
      541 gcgcaccctg ccctcccgcg cggggaccat ccgcacctcc acattgcgcg tccggagccg
      601 gagccagggg tacacgttgg agggatactc gacatttgcc gtgaccaggt tgtccccttc
      661 cttccacggg aaaccggccg cgacgaagga gagcccctcg gaggtgttct tcacgaaggc
      721 gacctcgtcc ggggaggcgc cgacgatccg cgcgaaccgg tcccgggtct cgttggccag
      781 ctcctcccat ttccgcaggc ggtacgcccc ctcgtcccgg gcaagctgca acatgcggat
      841 cccggcctcg gcggcgcgcg ccgggatcgg cgacacgccc gcgtggttga ggtacaggaa
      901 attctcggtc accggaaatt ccgcggcccg gatcggccca acgtcgatcg gcaaattgtc
      961 cccctccggc cggcctattc cgggcccggc ctggttatac gttataatat ttccatggat
     1021 tggtcagggg aaaacacgct gcgctacagc cggaacatcc tcgtcgagga actgggggag
     1081 gccgggcagg agcggctctt cgcgtcgtcc gcgctcgttg tcggcgccgg gggattgggg
     1141 tcgcccgctc tcctctatct cgccgccgcg ggaatcggac ggatcggggt gatcgacgac
     1201 gaccgggtcg acatcaccaa tctcaaccgc caggtgatcc accgggccga tgccgtgggg
     1261 caatggaagg ccgactccgc ggccgcggcg ctgcgggcgt tccggccgga cctggcggtc
     1321 gacgtgtacc cggaggcgct caccgcggcg aacgccgccg cgctgttccg gaggtacgac
     1381 gcggtcgtgg acgccagcga caacttctcc acgaagtacc tgtgcaacga cgcggcggtg
     1441 gtgagccgcc gaccgctcgt ccacgccggt gtcctgcgat tcggcggcca gatgctcacg
     1501 gtcgtccccg gcgcgggacc ttgcctgcga tgtgtgatcc cccgcatccc ctcgaggaag
     1561 gactcccccg ggtgcgccga ggccggcatc atcggcgcgg cggcaggtgt cctcggcgcg
     1621 tggcaggcga tggaggcgat caagctgctc tcgggaaccg caccgtcgcc cgggggaaaa
     1681 ctgctgacca tcgacacgct ctcgaacgag gtgtcgtgcc tcacggtagt ccgcgaccgg
     1741 tcgtgccccg cgtgcggaga acatccccgg atcacggaac cgctgtcggc cgccgaatac
     1801 gatcatgatc ggatgtgtgc cggatgaacc tccccgccac cctctccccc ccacggaagg
     1861 cggtcgttct cctctcgggc gggctcgaca gcaccctggc ggcccggatc gtccaagacc
     1921 aggggatcga gcttcacgcc atccatttca catcgccgtt ctgcacctgc tctcacaacg
     1981 gtccggggga cgggagggga tgccgttcgg cggcgcagga ggtcgcggtg gggatgggga
     2041 tcccgatccg gaccgtgtcg aagggcgagg agtacctcga aatcgtcaag cacccccggc
     2101 acggccgggg atcggcgatg aacccgtgca tcgactgccg gatcttcacc ctgcgaaagg
     2161 cgaaggcgtt catggaggag atcggggcct ctttcctggt cacaggagaa gtcgtcggac
     2221 agcgaccgat gtcccagcgg gacgacacgc tgcgcggcat cgagaagcac agcggatgcg
     2281 cggggatcat cctgcgcccg ctttccgcgc tccacctccc ccccacgctg ccggagcggg
     2341 aaggctgggt ggaccgggag aagatgctct ccatcaccgg gcggtcccgc aaggagcaga
     2401 tccggctcgc gaaggagttc aagatcggcg actatccgtg ccccgcgggg ggatgcatgc
     2461 tgaccgaccg gaccttctcc atcaaggtgc gggatctcct cgaccatcac ccttctttcg
     2521 ggatgcacga agtcctcctc ctgaaggcgg gacgccattt ccgcgttcag gggatgaggg
     2581 cgatcgtggc caaaagcgag gaggagaacc gccggctcga ggccctctgc cgggggaggg
     2641 acaccgtcta cgttgcgcac agccacccgg gtccttccgt tacgctgctg ggcggaagcg
     2701 aggaggaacg cctcgacctg ctctcccgga tcttcacccg gtacggcaag gcggggtcgg
     2761 tcgggccgta cgagatccgg gagatttccc ccgacgggga gcgactcgta accgtgcgtg
     2821 aaaacccgga cttcgaggag gtagggcgtg gactactctg ctgaaacctg gaacggcgct
     2881 ccgacgccgg acgacctgcg ccgctacgag gcgctgctcg cggcgctccg ccggatggga
     2941 tccgccgtgg tcgccttcag cggcggggtc gacagttcgt ttctcctcca cgccgcgaag
     3001 gaggcgatgg gagagcgggt cctggccgtc accgccacct ccccgacgta cccccgctcc
     3061 gagcgggagg aggctgtgcg gctcgcgcgc tcctggggag tccgtcacca cctcgtcgaa
     3121 tcgaacgagc tggagatccc cggattctcc gcgaaccctc cggaccggtg ctaccactgc
     3181 aaaaaggagc tcttcgggat tcttgccgga atcgcccggc gggaggggta cgcggccgtg
     3241 tgcgacgggt ccaacgccga cgacgtgcgc gacttccgcc cggggcgccg cgccgcgaag
     3301 gagctctccg tccgcagccc gctgctcgag aacggactgg gaaaaccggc gatccgccgc
     3361 ctgagccgaa gcttcgatct ccccacggcg gaccgcggtt cgttcgcatg cctctcctcg
     3421 cgcttcccgt acggaacaag gatcgacgag gagtcgctgc ggcgtgtcga ggcgtgcgag
     3481 gaggtgctgc gggggttcgg cttccgccag ttccgggtac gcgtccacga cgcggtggcg
     3541 cggatcgaag tggggatcga cgagatcccg cgcctcttcc agccggagat ctcggaggcg
     3601 gtccacgcgc ggttccggga gaacggcttt ctctacgtcg ccgtggacct caaggggtac
     3661 cggaccggct cgatgaacga ggggctgccg gcaatttccc cgggcctcgt cgcgggaccg
     3721 cccgaagacc tgtagccgac caataaggga ctccgggctc gtgccccccg tttttcctac
     3781 tgcgggggta ccccggtggc gcggagctcg taccggggcg ggggggcttc ctctctactc
     3841 cgccgattcc gccttccctt ccgtgggatc accctccagg tgcggcgatg ggggcggata
     3901 tcctgctaag caggagtgac gtagcggggg gttcctcgaa gcggcgtctg gagcgaagtg
     3961 gagacaggga tgtcgggaac gagcggaggc gcaggcgagg agaccatgga tggtcgacga
     4021 gccgtagcgg agaggaagcc ccccgggagg aaccctgcgt tcccacgctc cagaggtgca
     4081 atgtagcgag gaagggggta tcccgcggag attaaagcgc ctcccgcatc gcctttcagt
     4141 ttttcaggat gttcgacttg aagatcagct tctcgtggtc gtcgccgtct tccgggaacg
     4201 acgcgtggcc gaaccgcacc tccacgcgcg catcccttgc gcatcgttgg atctcctcgg
     4261 cctcggcggc gatcgccttc ttgatccgcg ggatcgcgct gatcttgccg tcctccgcct
     4321 cgggcaggat catcccgaac ttcacctcgc tgatccgcgc cacgatgtcg tactcgcgaa
     4381 gagaggcgcg gatcgccttc gccacctgct tcatcatcca atccgcgcgc cgcgcgcggg
     4441 attgcgaccc tgacgggacg tgcgactgga cctcgcagat catcaggacg aggcgccgct
     4501 ggaaccgcct cgcccggctg atctcgtgca gcaggcgctc ctggaaatat ttcagggtgg
     4561 gcagcgaggt cgcctcgtcg aggttcttca gccgatcgtt ccgctcgtag gcgatcgcgt
     4621 tgacgaccgc cttctcggcg tacttgagga atttcccgaa cgtggcgagg tcctcggtag
     4681 tgaacgagga gggaaagaac gtcttgtgcg ggaacttgtc gaagatcgtg acggtcccga
     4741 ggatctcccc gtccccgtgg aggggaacgc acgcgagcgt ccgggcgacc ccgacgaagt
     4801 cctgccagcc gggctcttgc gaggcgtcgc gcacgagcac ggacggctcc ccc
//
LOCUS       DASVJL010000069         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_571794, whole genome shotgun sequence.
ACCESSION   DASVJL010000069 DASVJL010000000
VERSION     DASVJL010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571794"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..>1785)
                     /locus_tag="VF357_01235"
     CDS             complement(116..>1785)
                     /locus_tag="VF357_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7522797.1"
                     /translation="FREFEAAFPHEETPDQEQVIREVLSDLSSPRPMDRLVCGDVGYG
                     KTEVAIRAAFQVVMAGKQAAVLVPTTILAEQHYQTFTRRLAGYPVRVENLSRFGTRKN
                     QAEVVKRLANGTVDVVIGTHRLLQRDVAFRNLGLVVIDEEQRFGVAHKERLKRMRAAV
                     DVLTLSATPIPRTLHMAFSGIRDVSLIATPPEDRLSIRTFVVPFSGETIREAVDREIR
                     RGGQVFFVHNRVQTLPAMERYLRELLPDARIAVGHGQMDEETLSVAMDDFASRRADIL
                     LCSAIIEAGLDLPNANTILVNHAHRFGLAQLYQLRGRVGRDRHRAYAYFVVPKDVALT
                     RDATRRLAVLEELTELGSGFRIASHDLEIRGAGNLLGKDQSGQIHQVGYELYTQLLSE
                     AVEEISGKASSQEEEPELELRVPAFLPDDYIDEAGERLEFYRKLSSAKTVDDADEIEM
                     ALLDRFGRLPVPARALCDLARMRAAIRSAGVAELKRGDGSLFLTLSAHSPFDRANLVS
                     WVTRERKTFSFVRGEILAMRLPGQDSTEILAAAKNLLNRFGTGSSIG"
BASE COUNT          291 a          630 c          586 g          278 t
ORIGIN      
        1 tcccgggggc gctcccgcag gcggatgcga caaacgcgaa aatcacgaac agaaccggca
       61 tcttccgcat ggagcggctc tccttggcga aaaagtgtcg aagggatgcg atccgtcatc
      121 ctatgctact gcccgtaccg aaccggttca acaggttttt cgccgccgcc aggatctccg
      181 tggaatcctg gccgggaagg cgcatcgcga ggatctcccc gcgaacgaag gagaaggtct
      241 tccgctcccg cgtcacccag gagaccaggt tcgcccggtc gaacggggag tgggcggaga
      301 gggtgaggaa cagggagccg tccccgcgct tgagctccgc cactcccgcg gagcggatcg
      361 cggcgcgcat ccgcgccagg tcgcacagcg cgcgggcggg gacgggaagc cgaccgaacc
      421 ggtccagcag cgccatctcg atctcgtccg cgtcgtccac ggtcttcgcc gacgacagct
      481 tccggtagaa ctccaggcgc tccccggcct cgtcgatgta gtcgtccgga aggaacgccg
      541 ggacgcgaag ctccagttcc ggctcctcct cctgcgagga ggccttcccc gagatctcct
      601 ccaccgcctc ggagagcagc tgcgtgtaca gctcgtaccc cacctggtgg atctgtcccg
      661 actggtcctt cccgagaagg ttccccgcgc cccggatctc gaggtcgtgg gaggcgatcc
      721 ggaatcccga gccgagctcg gtgagctcct cgagcaccgc cagccgccgg gtggcatccc
      781 tggtgagggc cacgtccttc ggtacgacga agtacgcgta cgcgcggtgg cggtcgcggc
      841 ccacgcggcc ccggagctgg tacagctggg ccagcccgaa ccggtgcgcg tggttcacga
      901 ggatcgtgtt cgcgttggga agatccagcc cggcctcgat gatcgcggag cacaggagga
      961 tgtcggcgcg gcgcgacgcg aagtcgtcca ttgcgaccga aagggtctcc tcgtccatct
     1021 gcccgtgccc cacggcgatc cgcgcgtcgg ggagaagctc gcgaaggtac cgctccatcg
     1081 cgggcagggt ctgcacccgg ttgtggacga agaagacctg cccgccccgg cgaatctccc
     1141 gatccaccgc ctcgcggatc gtctcccccg agaaggggac gacgaaggtg cggatcgaga
     1201 gccggtcctc ggggggggtg gcgatcaaac taacgtcccg gatcccggag aacgccatgt
     1261 gcagcgtgcg cgggatcggc gtggccgaca gcgtcagcac gtcgacggcg gcgcgcatcc
     1321 tcttcaaccg ttccttgtgc gccactccga accgctgctc ctcgtcgatc accacgagcc
     1381 cgaggttcct gaacgccacg tccctctgca gcagccggtg cgttccgatg acgacgtcca
     1441 ccgtgccgtt cgccagcctt ttcaccacct ccgcctggtt cttccgggtg ccgaaccgcg
     1501 acaggttctc cacccgcacg gggtaccccg cgaggcggcg ggtgaacgtc tggtaatgct
     1561 gctcggccag gatggtcgtc ggaaccagca ccgccgcctg ctttcccgcc atcaccacct
     1621 ggaacgccgc gcggatcgcc acctcggtct ttccgtaccc cacgtcgccg cagacgagcc
     1681 ggtccatcgg gcggggggag gagaggtcgg acagcacctc gcggatcacc tgctcctggt
     1741 ccggcgtctc ctcgtggggg aacgccgcct cgaattcccg gaaga
//
LOCUS       DASVJL010000070         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_478682, whole genome shotgun sequence.
ACCESSION   DASVJL010000070 DASVJL010000000
VERSION     DASVJL010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478682"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VF357_01240"
     CDS             <1..257
                     /locus_tag="VF357_01240"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX7522798.1"
                     /translation="LEGMPAVLAGEITRTLTIPTIGIGAGAGCDGQVLVMHDLLGLFD
                     EFRPKFVKRFGELRKPVEDAVGAYAAAVRDGSFPGKEHSF"
     gene            257..1105
                     /gene="panC"
                     /locus_tag="VF357_01245"
     CDS             257..1105
                     /gene="panC"
                     /locus_tag="VF357_01245"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX7522799.1"
                     /translation="MERLRTPAAMRVWADARRAEGARIGLVPTMGYLHDGHVSLVRVA
                     RGAGSEAVAASIFVNPTQFGPGEDFEKYPRDEARDLAMLDAAGVDAVYLPGVRDMYPE
                     GHQTFVEVTGVSRELCGAARPGHFRGVATVVAKLFLAAKPHVAVFGEKDCQQLAVIRA
                     MTRDLDFGVEIVGAPIVREEDGLAMSSRNAYLSGDDRIAARCLSRGLFRAKELFGKGE
                     RDAETLVAAAGAAIEAEPRAKPEYAEGRDPVTLTPLSGRVDSVTILVAAKVGPARLID
                     NITLGK"
     gene            1125..1529
                     /gene="panD"
                     /locus_tag="VF357_01250"
     CDS             1125..1529
                     /gene="panD"
                     /locus_tag="VF357_01250"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX7522800.1"
                     /translation="MIKTMLKCKIHRATVTDAVLHYEGSVTIDKTLMDAAGLVEYEQV
                     HIWNVDNGNRFTTYAIDGEADSGVICLNGAAARQVSKGDLVIIAAFSAYDEKELEHYQ
                     PSLVYVDGKNRITSVMRKIESESQRPRVAAAV"
     gene            1582..>2556
                     /locus_tag="VF357_01255"
     CDS             1582..>2556
                     /locus_tag="VF357_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7522801.1"
                     /translation="MKSGPDRQQGVVYTASRLVAEAGATFSPGAVAVADGSVLLAGAK
                     ADVLRAAPAGFPRMEFPGEVILPGLVNAHTHLQIPRLTDGAGVPLPIPASFVDWILRV
                     IAWRLGSDPASFASNFAAAAAEALSFGTTTVGEIAGPDLSSYGGCPLRARVYAEGIGF
                     APNVSSEVLALVGAAIDRLEKISAENPLVRAGVSPHTLYTVGESLLRSLADLAAARGL
                     PACLHLAESTPETAFLTDGGGEIATRLYPAVGKDVSWFGGLGRTIPAYLAETGILREG
                     VLLVHNVHLARPEIDALRAGGARFILCPRSNAAHGNGAPDVTHFVDAKI"
BASE COUNT          405 a          803 c          945 g          403 t
ORIGIN      
        1 tgctcgaggg gatgccggcc gttctcgccg gggagatcac gcgtacgctt acgatcccga
       61 cgatcgggat cggagctggg gcgggctgcg acgggcaggt gctggtgatg cacgacctgc
      121 tggggctgtt cgacgagttc cgcccgaagt tcgtaaagcg cttcggcgag cttcgaaagc
      181 cggtggagga tgcggtcggg gcgtacgccg ccgcggtgcg cgacgggtcg ttcccgggaa
      241 aggagcattc cttctgatgg agcggcttcg caccccggcg gcgatgcggg tgtgggccga
      301 cgcgcggcgg gcggaagggg cccggatcgg cctggtgccg acgatggggt acctgcacga
      361 cggccacgtc agcctggtgc gggtcgcgag gggggccggg agcgaagcgg tggcggcgtc
      421 gatcttcgtc aacccgacgc agttcggacc gggcgaggat ttcgaaaagt acccccggga
      481 cgaagcgcgg gacctggcga tgctcgatgc ggcgggagtc gatgcggtgt acctccccgg
      541 cgtgagggac atgtacccgg aggggcacca gacgttcgtc gaggtgaccg gtgtttcgcg
      601 ggaactgtgc ggcgcggcgc ggcccggaca cttccgcggc gttgccacgg tggtcgcgaa
      661 gctgttcctc gcggcgaagc cccatgtggc ggtcttcggg gagaaggatt gccagcagtt
      721 ggccgtgatc cgcgcgatga cgcgggacct agacttcggc gtcgagatcg tcggggcgcc
      781 gatcgtccgg gaagaggacg gactggcgat gagctcgcgc aacgcctatc tttcgggcga
      841 cgaccggatc gcggcccggt gcctgtcgcg ggggctcttc cgcgcgaaag agctgttcgg
      901 gaagggagag cgggacgcgg agaccctggt cgcggcggcc ggggcggcga tcgaagcgga
      961 gcctcgggcg aaaccggaat acgcggaagg gcgggacccg gtcacgctca ccccgctttc
     1021 gggccgggtc gactccgtca cgatcctggt ggcggcgaag gtcggccccg cgcggttgat
     1081 cgacaacatc acgctgggaa aataatgaag gggagggacg catcatgata aagaccatgc
     1141 tgaagtgcaa gatccatcgc gcgacggtga ccgatgccgt gctgcactac gaggggagtg
     1201 tgacgatcga caagacgctg atggacgcgg cggggctcgt cgaatacgag caggtgcaca
     1261 tctggaacgt cgacaacggg aatcggttca ccacctacgc catcgatggc gaggcggact
     1321 ccggcgtgat ctgcctgaac ggcgccgccg cccgccaagt gagcaagggc gacctggtga
     1381 tcatcgccgc cttctccgcc tacgacgaaa aggaactcga gcattaccag ccgtcgctgg
     1441 tctacgtgga cgggaagaac cggatcacaa gcgtgatgcg gaagatcgag tccgaaagcc
     1501 agcgcccgcg cgtcgccgcc gcggtctgat cccggacccg ggacgctcct gcatgcgtca
     1561 ggagggcagg atgctgcaac catgaagtcg ggacctgacc ggcagcaggg ggtcgtgtac
     1621 accgcctcgc ggctggtcgc ggaggccgga gcgacgtttt cccccggcgc cgtggccgtg
     1681 gcggacggga gcgtcctgct ggcgggggca aaggcggacg tcctccgggc cgctccggcg
     1741 ggcttcccca ggatggagtt ccccggcgaa gtgatcctcc ccgggctggt caacgcgcac
     1801 acccacctgc agatcccgcg gctcaccgac ggcgcggggg tgccgctgcc aatccccgcc
     1861 tcgttcgtcg actggatcct ccgcgtgatc gcctggaggc tggggtcgga cccggcctcc
     1921 tttgcgtcga atttcgccgc cgcggccgcc gaggcgctct ccttcggcac cacgacggtg
     1981 ggggaaatcg ccgggccgga cctttcctcg tacggcggct gtcccctgcg ggcgcgagtc
     2041 tacgccgaag ggatcggctt cgctccgaat gtctcctccg aggtcctcgc cctggtcggc
     2101 gcggcgatcg accggttgga gaagatctcc gcggaaaacc ccctcgttcg ggcgggagtc
     2161 tctccccaca cgctgtacac ggtcggggaa agccttcttc gctccctcgc ggatctcgcc
     2221 gccgcaaggg ggcttcccgc ctgtcttcat ctggcggagt cgacgccgga gacggcattt
     2281 ctcaccgatg ggggagggga aatagcgacg cgtctttatc cggcggtggg gaaggacgtt
     2341 tcctggttcg gcgggctcgg acggacgatc cccgcgtacc tcgccgagac cggtattttg
     2401 cgcgaagggg tgctgctggt gcataatgtc cacctcgcgc gtccggagat cgacgcactt
     2461 cgcgcgggcg gcgcccgctt catcctttgc ccgaggagca acgcggcgca cggcaacggc
     2521 gccccggacg tcacccattt cgtggacgcg aagatc
//
LOCUS       DASVJL010000071         5258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1416904, whole genome shotgun sequence.
ACCESSION   DASVJL010000071 DASVJL010000000
VERSION     DASVJL010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416904"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /gene="dxs"
                     /locus_tag="VF357_01260"
     CDS             complement(<1..1794)
                     /gene="dxs"
                     /locus_tag="VF357_01260"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX7522802.1"
                     /translation="MTVTDAPRPVAIQSPAELKKIPRDKLPGVAAELRETIVRNIART
                     GGHLASSLGVVELTIAIHYVFDCPQDRIVWDVGHQAYAHKILTGRRDVFPTLRTFGGI
                     SGFPRISESPCDAFGTGHSGTSISAALGMAVARDLRKGKNRVVAVIGDGSLSSGLALE
                     GLNHAGHQKRDLIVILNDNEWSISQNVGAISGYLNRMMTGRLYTSFRKRVETLLKSMP
                     HGAFLARIAKKSEELTKGFIVPGLLFEELGYTYIGPIPGHDLEALIGTLQNLENIEGP
                     VLVHVVTSKGKGYAPAEANPEYFHGVGAFDPETGKGIGKAAAPTYTDVFSDAIVELAR
                     ENPKVVAITAAMCGGTGLTKFREAFPDRFFDVGIAEAHAVTFAAGLAREGKIPVVAIY
                     STFLQRAFDQIIHDVCIQKLPVVFAVDRGGLVGADGATHQGLFDLSFLRQIPEMSLMA
                     PRDEAELVRMLRTAVGAGCPVAIRYPRGSGTGAAIPSGGEAIPWGKGELLEEGKDVLL
                     VGIGSTVLTCLHAAQELRKQGVSAAVVDARFVKPLDADLLLPLVRRIGRVVTVEENLL
                     AGGFGSAVMELLEEHDEHAQRFIRIGVHDRFV"
     gene            complement(1791..2681)
                     /locus_tag="VF357_01265"
     CDS             complement(1791..2681)
                     /locus_tag="VF357_01265"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX7522803.1"
                     /translation="MTPADTIAGLRGLVERSLAGTFASEASRIPSPLREAMEYSLMAG
                     GKRVRPVLLLSAGVAVGGEEGDLLPFACAVEYIHTYSLIHDDLPAMDDDDFRRGKPTS
                     HKVFGEGTAILAGDALLTDAFRVMAQSPLAQADPGRAVRAIADLAQAAGGGGMVGGQQ
                     MDLSAAPGSSTAAEVDEIELRKTAALLASCARMGAILGGGSPAQVDALGKYGTRLGLL
                     FQVTDDILDETGSFEEMGKGIAKDRARGKWTYPVAHGMPAAVERASELERAALDAVST
                     FGAEADPLRELVGMVRNRRK"
     gene            complement(2678..2932)
                     /gene="xseB"
                     /locus_tag="VF357_01270"
     CDS             complement(2678..2932)
                     /gene="xseB"
                     /locus_tag="VF357_01270"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX7522804.1"
                     /translation="MAGKAKEPSFEEALKGLEAVVERLESGEPPLEESIRLFEEGMRL
                     SETCRKRLDEADRKIELLLRKPGGVSRETAEEDDILGKEE"
     gene            complement(3019..3849)
                     /locus_tag="VF357_01275"
     CDS             complement(3019..3849)
                     /locus_tag="VF357_01275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7522805.1"
                     /translation="MIAGAPAIAAPDGTLSIAVSTRTPALGDPVVVEVAANGAVDNLV
                     LRWKRVAWPMRKIAPGRYEGLIGVDLDDPVGPAVVAADGFLDGARVRAEAEVTISPRK
                     FDVQELSLPKGMAEFDNATLVRIRAEEAELSRRFSRVTPPRWRIPFLHPVEDYRPANF
                     GARRVINGDPRMPHSGVDLHLPGGTPVRAIADGRVAFAGEQFFGGRSVVIDHGGGVFS
                     VYYHLKEFSVAEGQEISRGDRVGSVGATGRATGPHLHFSVRVPGGRVDPTRLFALPGR
                     "
     gene            complement(3897..5255)
                     /gene="xseA"
                     /locus_tag="VF357_01280"
     CDS             complement(3897..5255)
                     /gene="xseA"
                     /locus_tag="VF357_01280"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX7522806.1"
                     /translation="MEPDLFAASPSGDVLSVSELTGKIKRLLESSFRTVRVEGEVSNY
                     KRYEASGHRYFSLKDEGAQIRVVLFRGNERNILGEIRDGQTVIVTGSLGVFEKKGEYQ
                     IYARSAEVRGAGNLLVELERRKRRLAEEGLFDAARKRPLPPFPRRLGIVTSLHGAVLR
                     DMVRVARSRFPGVEIVLAPSLVQGEGAAAEIAAALDTLYASGGADVVIVGRGGGSIED
                     LWAFNEEEVVRAIVRSPVPVISAVGHETDFTLADLAADHRAPTPTAAAQMAVPDRVEL
                     LDRVAALSLRARRADARSRETARQEWRIAAGKLSDPRPLLQGKRYAVDALSSSLSELA
                     RSAVRDGRDAVERLSASVRIHSPAAWVSGRRGELAVLLGRARSSADARNHRLRSDLDV
                     LGGKLASLNPTAVLTRGYAIALDRATGKAVRSVSEARPGRALEIRVSDGAFGAVVTEP
                     KT"
BASE COUNT          866 a         1911 c         1677 g          804 t
ORIGIN      
        1 gacgaaccgg tcgtggacgc cgatccggat gaatcgttgg gcatgctcgt cgtgctcctc
       61 gagaagctcc atcaccgcgc tgccgaaccc gcccgcgagg aggttctcct ccacggtgac
      121 gacccgcccg atccggcgga ccaggggcag aaggaggtcg gcgtcgaggg gctttacgaa
      181 ccgggcgtcg accaccgccg cggatacacc ctgtttccgc agctcctgcg cagcgtgcag
      241 gcatgtcaag acggtcgaac cgatccccac gaggagcaca tccttccctt cctcgagaag
      301 ttcgcccttc ccccagggaa tcgcctcgcc cccggagggg atcgccgcac ctgtcccgga
      361 accccgcgga tagcgaatcg ccacgggaca gccggcgcca accgccgtac gcagcatccg
      421 gaccaactcg gcctcgtcgc gtggcgccat gagcgacatt tccgggatct gccgcaggaa
      481 cgacagatcg aacagcccct ggtgcgtggc cccgtcggcc ccgacgagac cgccgcggtc
      541 gacggcgaac accacgggga gcttctgaat gcagacgtcg tggatgatct ggtcgaaagc
      601 gcgctgcaga aaggtcgagt agatcgccac cacggggatc tttccctctc gggcaaggcc
      661 cgcggcgaag gtcaccgcgt gggcttccgc gatccccacg tcgaagaacc ggtcgggaaa
      721 cgcctcgcgg aatttcgtaa ggcccgtccc gccgcacatc gccgcggtga tggcgaccac
      781 cttcgggttc tcccttgcga gctcgacgat cgcgtcggag aagacgtccg tgtaggtcgg
      841 cgccgccgcc ttcccgatcc ccttcccggt ctccgggtcg aaggcgccca ccccgtggaa
      901 atattccggg ttcgcctccg ccggcgcata tcccttcccc ttggacgtca ccacgtggac
      961 aagcacgggc ccttcgatgt tctcgaggtt ctggagcgtt ccgatgaggg cctcgaggtc
     1021 gtgccccggg atcgggccga tgtaggtgta gccgagctcc tcgaacagga ggccggggac
     1081 gatgaacccc ttcgtcaact cctccgactt tttcgcgatc cgcgccagga acgcgccgtg
     1141 gggcatcgat ttcaggagcg tctcgacccg cttccggaac gaagtgtaca gtctcccggt
     1201 catcatccgg ttgaggtatc cggaaatggc gccgacgttc tgggagatcg accactcgtt
     1261 gtcgttgagg atgacgatga gatcgcgctt ctggtggccc gcgtggttca acccttcgag
     1321 ggcgagaccg gaggagaggg agccgtcccc gatgacggcg acgacacggt tctttccctt
     1381 ccggagatcc cgggcgaccg ccatcccgag ggccgcggag atcgacgtgc cggagtgccc
     1441 cgtgccgaag gcgtcgcagg ggctctcgga gatgcgagga aatccggaga tccccccgaa
     1501 ggtccgcagc gtcggaaaga cgtcccgcct ccccgtgagg atcttgtggg cataggcctg
     1561 gtgcccgacg tcccatacga tccggtcctg cgggcagtca aacacgtagt ggatggcgat
     1621 cgtaagttcc accacgccga ggctcgaagc gaggtgcccc ccggtgcgcg cgatgttgcg
     1681 tacgatcgtc tcccgcagtt ccgcggcgac gcccggaagc ttgtcgcgag gtatcttctt
     1741 cagttccgcc ggcgactgga tcgccaccgg ccggggagca tccgtcaccg tcacttcctc
     1801 ctgttccgca ccatgccgac gagttcccgg agcggatcgg cttccgcccc gaacgtggaa
     1861 accgcgtcga gagcggcccg ttcgagctcc gaggcccgct cgaccgcggc cggcatcccg
     1921 tgggccacgg ggtaggtcca cttcccccgc gcccgatcct tggcgatccc cttccccatc
     1981 tcctcgaagc tccccgtctc gtcgagaatg tcgtcggtta cctggaaaag gagacccaac
     2041 cgcgtgccgt acttccccag cgcgtcgacc tgcgccgggg atccgccgcc gaggatcgct
     2101 cccatccgcg cgcaggaggc aaggagcgcg gcggtcttcc gcagctcgat ctcgtcgacc
     2161 tcggcagccg tggaactccc gggcgcggcg gacaggtcca tctgctggcc gcccaccatt
     2221 ccgcccccgc cggccgcctg cgcgagatcg gcgatcgccc gcaccgccct tcccgggtcg
     2281 gcctgcgcca agggggattg cgccatcacc cgaaacgcgt ccgtcagcag cgcgtcgccg
     2341 gccaggatcg ccgtcccctc accgaacacc ttgtgggagg tcggcttccc ccgccggaag
     2401 tcgtcgtcgt ccatcgccgg caagtcgtcg tggatcagcg agtacgtgtg aatgtactcg
     2461 accgcgcagg cgaacggaag aagatctcct tcttctccac ccaccgccac gccggcggac
     2521 aggagcaata ccggccgcac gcgcttcccc cccgccatca gggagtactc catcgcctcc
     2581 cggaggggag acgggatccg ggacgcttcg gaagcgaagg ttccggccag gctcctctcg
     2641 accaacccgc gcagaccggc gatggtgtcg gccggcgtca ctcttccttc cctaagatat
     2701 cgtcttcctc cgcggtctcc cgggagacgc cccccggctt ccgcaggagg agctcgatct
     2761 tccgatcggc ctcgtcgagg cgctttcggc acgtctcgga gaggcgcatc ccctcctcga
     2821 acaggcggat cgactcctcg aggggaggct ctcccgactc gaggcgttcc acgacggctt
     2881 cgagtccctt caacgcctcc tcgaaggaag gctccttcgc tttacccgcc atcgcgcacc
     2941 gcctccgaag cgttcagccg cgcggcccgt gtgcggtcac gtcggtctga agcattgcct
     3001 ttcattatag ttcaccgctc accggcccgg aagggcgaac aggcgcgtgg ggtcgacgcg
     3061 cccgcccgga acccgcacgc tgaaatgcag atgcggcccc gtcgcccgac ccgtggcgcc
     3121 gaccgacccg acccgatccc cccgggagat ttcttgtccc tccgcgacgg agaactcctt
     3181 caggtgatag tagacgctga agaccccgcc gccgtggtcg atcaccaccg atcgcccgcc
     3241 gaaaaactgc tcgcccgcga aggcgacccg accgtcggcg atcgcgcgga ccggcgtccc
     3301 ccccggcagg tggaggtcca cgccggagtg cggcatccgg ggatcgccgt tgatcaccct
     3361 gcgcgcgccg aagttcgcgg gacggtaatc ctccaccgga tgaaggaacg ggatccgcca
     3421 acgtggaggg gtcacccggg agaacctccg ggatagctcc gcctcctccg cacggatccg
     3481 gaccagcgtg gcgttatcga attccgccat ccccttcgga agggacagtt cctgtacgtc
     3541 gaacttccgc ggagagatcg tcacttccgc ctccgctcgg acccgggccc cgtcgaggaa
     3601 cccatccgcg gccaccaccg ccggtcctac cggatcgtcg agatccacgc cgatcaaccc
     3661 ctcgtaccgt cccggggcaa tcttccgcat cggccaggcc acccgcttcc accggaggac
     3721 caggttgtcg acggccccgt tcgccgcaac ctccacgacc acgggatcgc ccaacgccgg
     3781 ggtacgggtc gaaacggcaa tcgacaacgt cccgtccggc gcggcgatcg cgggcgctcc
     3841 ggcgatcata ggtagcccat agcacaaggc aagaatcccc acccttcgta tgccgatcac
     3901 gttttcggct ccgtgacgac cgccccgaac gcgccgtcgg aaacccggat ctccagcgcc
     3961 cggccgggac gggcctccga tacggaacgg accgccttcc cggtggcgcg gtcgagcgcg
     4021 atcgcgtacc ctcgcgtcag cacggcggtc ggattgaggg acgccagctt gccgccgaga
     4081 acatcgaggt cggatcggag gcggtggttc cgcgcgtccg cggaggagcg tgcccggccg
     4141 agaaggaccg cgagctcgcc gcgccttccg gaaacccacg cggcggggga gtggatccgc
     4201 accgaggcgg agaggcgctc gacggcatcc cggccgtccc gtaccgcgga gcgcgcaagt
     4261 tccgagagcg aggacgacag cgcgtcgacg gcgtaccgct tcccctgaag gagggggcgc
     4321 gggtccgaca attttcccgc ggcgatccgc cactcctgac gggccgtttc ccgcgaacgg
     4381 gcatcggccc gacgcgcccg cagggagagg gccgcgaccc ggtcgaggag ctcgacccgg
     4441 tcggggacgg ccatctgcgc ggcggcggtc ggagtgggag cgcggtggtc cgcggcaagg
     4501 tcggccagcg tgaagtcggt ctcgtgtccg accgcgctga tgaccggaac gggggaacgg
     4561 acgatggccc gcacgacctc ctcctcgttg aacgcccaca ggtcctcgat cgaaccgccc
     4621 ccacggccga cgataacgac atccgcgccg ccggaggcgt acagcgtgtc gagcgccgcc
     4681 gcgatctccg ccgccgcgcc ttccccctgg accagcgacg gggcgaggac gatctccact
     4741 ccgggaaagc gggagcgcgc cacccgaacc atgtcgcgca ggacggcgcc gtgcaacgat
     4801 gtcacgatcc cgagtcgccg ggggaacggg ggaagcgggc gcttgcgcgc tgcgtcgaac
     4861 agcccctcct ccgcgaggcg acgcttccgc cgctcgagtt ccacgagaag gttcccggcg
     4921 ccccggacct ctgcggaccg tgcgtagatc tggtactccc ccttcttctc gaacaccccg
     4981 agggagccgg tgacgatcac ggtctgcccg tcgcggattt ccccgagaat gttccgctcg
     5041 tttccgcgga aaaggacgac gcggatctgc gccccctcgt ccttcaggga aaaataccgg
     5101 tgcccggacg cctcgtaccg cttgtagttc gacacctccc cctcgacccg tacggtccgg
     5161 aaggacgact cgaggagccg cttgatcttc ccggtgagct cactgacgga aagaacgtcc
     5221 ccggaaggcg atgccgcgaa aaggtcaggc tccactca
//
LOCUS       DASVJL010000072         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1229761, whole genome shotgun sequence.
ACCESSION   DASVJL010000072 DASVJL010000000
VERSION     DASVJL010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229761"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VF357_01285"
     CDS             complement(<1..242)
                     /locus_tag="VF357_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522807.1"
                     /translation="MLTKRGNGLSRRDFIRIAGGTLATLGLSGALTPKLARALEKAAA
                     GRPKVVWLHFASDTGCTESFIKSDHPSAGELVLDIL"
     gene            complement(467..808)
                     /gene="hypA"
                     /locus_tag="VF357_01290"
     CDS             complement(467..808)
                     /gene="hypA"
                     /locus_tag="VF357_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX7522808.1"
                     /translation="MHELGVASEILEVALSEADRHAAKKVTSIRLRVGVLRAIEPENL
                     SFLFEHLARGTLAEGAFLEIEEEPVRVECEACGVSEAPSFTLECPRCKGAGVSLTGGD
                     SLSILSLDVDA"
     gene            complement(809..1480)
                     /gene="hypB"
                     /locus_tag="VF357_01295"
     CDS             complement(809..1480)
                     /gene="hypB"
                     /locus_tag="VF357_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX7522809.1"
                     /translation="MRSDGNVRIDVERSILQSADEAAAAIRALLAERKIYALNLISSP
                     GSGKTTLIEALLSRFDGKGSIAVVEGDIETEIDAERIRKHGVQVRQINTRSSCHIQPS
                     RLLSVLREMDLSETRLLLVENVGNLVCPAEVSLGEDARVVLLSVTEGDEKPLKYPLVF
                     KTSDLLVITKTDLLPYVTFDVDRVRRTARVANPAVEIFEVSATTGVGMSALLDRIEAL
                     RATKN"
     gene            1610..2074
                     /locus_tag="VF357_01300"
     CDS             1610..2074
                     /locus_tag="VF357_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HEX7522810.1"
                     /translation="MRVIGVGNILLCDEGIGVHVVRELSSRGGMPGVEFVDGGVAGAT
                     LLNLVEGEERVVLVDAVDAPFPPGTVVRMTPDELTGSGTPAWSLHDLNLADTLGMMRL
                     RETLPEMILLGVVPADIETYRLELSDPLAARFGEIVEKVRSEIAAFAVSPRP"
     gene            2086..>2470
                     /locus_tag="VF357_01305"
     CDS             2086..>2470
                     /locus_tag="VF357_01305"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX7522811.1"
                     /translation="MGIAAIEITARGVVQGVGFRPFVHRLASRCGLAGWVENTPGSVV
                     LHIEGDAAALSRFRALFGSEIPPAARVTRLSVHKTAPTGVPGFTIRASRRDGIALSTI
                     PPDIATCPGCLRELGDPADRRHRYPF"
BASE COUNT          414 a          828 c          809 g          419 t
ORIGIN      
        1 ctcaggatgt cgaggaccag ctcccccgca ctcgggtggt ccgacttgat gaacgactcc
       61 gtgcacccgg tatcgctggc gaaatgtagc cagacgacct tgggcctccc cgccgccgcc
      121 ttttcgagcg cccgcgccag cttcggcgtc aacgccccgg acaacccgag cgtcgcgaga
      181 gttccccccg caatccggat gaaatcgcgc cgactgagac cgttcccccg cttcgtgagc
      241 atctgcgcct cctgcacggg cagcttggtg ttccataaaa tctaaaatat agttctattc
      301 ctgtcaagag aattgttctt tacaatcgag aacatataaa taggaatcga tagatggaca
      361 cagtcgacac gaaaatgata accgctgttc tattatttat tacaggtagg cggacagagc
      421 cggatcgtgc ggcatggggc tgcaattttc gtgacgggtt tacggttcag gcgtcgacat
      481 cgagggagag gatcgacagg gagtcgccgc cggtcaacga aacgcccgcc cccttgcagc
      541 gggggcattc caaggtgaag gaaggggcct ccgacacccc gcacgcctcg cattcgaccc
      601 ggaccggttc ctcctcgatt tcgagaaacg ccccttccgc gagtgttccg cgggcgaggt
      661 gttcgaagag gaaggagaga ttctccggct cgatggcgcg caggacgccc acgcgcagcc
      721 ggatcgacgt caccttcttc gccgcatgcc ggtccgcctc ggagagggca acctccagga
      781 tctcgctggc gaccccgagc tcgtgcactt agttcttcgt cgcccgcagg gcctcgatcc
      841 ggtcgaggag ggcggacatc cccacaccgg tggtagcgga gacctcgaag atctccaccg
      901 cggggttggc gacgcgcgcg gtgcggcgga cgcggtccac gtcgaacgtc acgtacggca
      961 gcaggtccgt cttcgtgatc acgagaaggt cggacgtctt gaagacgagc gggtacttga
     1021 gcggcttctc gtccccctcc gtcacgctca gcaggacgac gcgggcgtcc tcccccagcg
     1081 agacttccgc ggggcagacg aggttcccca cgttctccac gagaagaagc cgggtctccg
     1141 acaggtccat ctcccggagg acggacagaa gccgggacgg ctggatgtga caggaagacc
     1201 gggtgttgat ctgccggacc tgaacgccgt gcttccggat cctctccgcg tcgatctcgg
     1261 tctcaatgtc cccctcgacg acggcgatgc tccccttccc gtcgaaccgg gaaaggaggg
     1321 cctcgatcag cgtggttttt ccggaacccg gcgaggagat gaggttgagc gcatagatct
     1381 tccgctcggc gaggagggcg cggatcgccg cggccgcctc gtccgcagat tgcaggatcg
     1441 accgctccac atcgatgcgc acgttgccgt cggatcgcat gcgggtcagg ataccacacc
     1501 ggtcgcgcgg gcccattctg ctacactgag gctttgcgac ggaggggggc gccccactac
     1561 gagcttcgca ctggtggggt acccccgcaa ccgtaatacc gagggggcaa tgcgggtcat
     1621 cggggtcggg aacatccttc tttgcgacga ggggatcggt gtccacgtcg tgcgggagct
     1681 ctcctcccgg ggaggtatgc cgggcgtgga gttcgtggac ggcggggtgg ccggggccac
     1741 cctcctcaac ctggtcgagg gggaggaacg cgtcgtcctg gtggacgcag tcgacgcccc
     1801 gttcccgccg gggacggtcg tgcggatgac ccccgacgaa ctgaccggga gcggcacgcc
     1861 cgcctggtcc ctccacgacc tgaacctcgc cgacacgctc gggatgatgc ggctgcgcga
     1921 gaccttgccg gagatgatcc tcctcggcgt ggtccccgcc gacatcgaaa cgtaccgcct
     1981 cgaactgtcg gatccgctcg cggcgcggtt cggggaaatc gtcgaaaagg tgcggtccga
     2041 gatcgcggcg ttcgcggtct ccccccgccc gtaacgggcc gccccgtggg gatcgcggcg
     2101 atcgagatca ccgcccgcgg cgtggtgcag ggggtgggat tccggccgtt cgtccaccgg
     2161 ctggcttcgc ggtgcgggct cgccgggtgg gtcgagaaca ccccggggag cgtcgtcctc
     2221 catatcgaag gggacgcggc ggcgctctcg cgctttcgcg ccctcttcgg ttcggagatc
     2281 ccccccgcgg cgcgcgtgac ccgtctctcc gtccacaaga ccgcgcccac cggcgtcccg
     2341 gggttcacga tccgggcgag ccgccgcgac gggatcgccc tctccacgat ccccccagac
     2401 atcgcgacgt gccccggatg cctgcgggaa ctcggcgatc ccgcggaccg ccgccaccgg
     2461 tacccgttca
//
LOCUS       DASVJL010000073        11373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_104449, whole genome shotgun sequence.
ACCESSION   DASVJL010000073 DASVJL010000000
VERSION     DASVJL010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11373
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104449"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VF357_01310"
     CDS             complement(<1..570)
                     /locus_tag="VF357_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7522812.1"
                     /translation="MSGNWAKAFSRGSGGRGREARTVVRDIEVAFQVKLREYTTIGIG
                     GAADRLVFPRSAAQVREAVVAEGRSGRTVHVLGAGSNLLVADRGVSATVLCTKKHLSK
                     VVFLSDGAVVVEAGAMLPRLAVLCALSGLSGMESLSGIPGTVGGALSMNAGAYGQSIG
                     ELVEWVEVVDVEGEIHRVEARAIRFGYREA"
     gene            complement(533..1900)
                     /gene="murC"
                     /locus_tag="VF357_01315"
     CDS             complement(533..1900)
                     /gene="murC"
                     /locus_tag="VF357_01315"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX7522813.1"
                     /translation="MYRKGLPIHFVGIGGIGMSGIAELLLNLGYRVSGSDLRRSDTTE
                     RLERLGAAVRTGHSASNVPADGHVVVVSSAVRPDNPEVLEAHRRKIPVIPRAEMLAEL
                     MRMKYGIAIAGTHGKTTTTSMVATVLATAGWDPTAVVGGKLNSLGSNAKLGQGEFLVA
                     EADESDGSFLKLSPTVAVVTNIDPEHLDFYSGIGQIKETFLHFINKVPFYGFSVLCID
                     HPNVQELIPSVEKTYVTYGFSRSADYRAEEVVVSGMTNRFAVFARGERLGEVLLRAPG
                     RHNVSNALAATAVALELRIPFEQVREGLSLYAGVGRRFQVKGEIDGVTVVDDYGHHPV
                     EVRATLAAAREVWPDRRIVLGFQPHRYSRTRALFKEFLSAFQDADVLLVFEVYSAGEE
                     PIEGATGKDLCSAVRDYGHREARYLGKSAGAREAVRATLRPGDIFLTMGAGDVWKLGE
                     SLLPR"
     gene            complement(1893..3002)
                     /locus_tag="VF357_01320"
     CDS             complement(1893..3002)
                     /locus_tag="VF357_01320"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEX7522814.1"
                     /translation="MMIAGGGTGGHVFPGIALADAFLSLCPDGAVSFVGTREGLEAWA
                     VPARGFPIDFVPSGQVRGKGIGALPGALRMALGFFAGVAVLRRRRPDLVFGVGGYASV
                     PVALSAALLKIPLFLQEQNSVPGRSNRFLARRAVRVFAGFPGAVPFFPPGRVEVTGNP
                     VRPEIVAAARERGRSFPPETPFTVLALGGSQGARAINTRVLGMARRAKREGTAIRFLL
                     QTGTREYEAVARSAGDEALPVEPFPFTDRIGDWFPRCHAVLMRGGALSIAEAALFGRP
                     CVLVPYPFAADDHQEGNAREFCASGAGAWLPEGEATEEALWSTLWKLGGDRDARERAG
                     AAAVSFSRPDAAVEAVRAALRLTGNVIGEGVGPNV"
     gene            complement(2944..4125)
                     /gene="ftsW"
                     /locus_tag="VF357_01325"
     CDS             complement(2944..4125)
                     /gene="ftsW"
                     /locus_tag="VF357_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX7522815.1"
                     /translation="MILGKRHENLILALCALSLTVLGMIMIYSATNVMAGSSARYGFD
                     PAYFFKRQVLFLAIGIAFAMGLSRVDHDFYRRRIGWIVGVAFLLLLLVYVPGVRHTAN
                     GASRWINLRLFTFQPSEFAKFALLAFAAWAIDRNGEKPKNEYRAFFPMLAVMGLFVAV
                     ILKEPDFGMAVVIIASFLVIVFIAGFPWKLLAAFAAAGVMGGALLILAKPYRVARIQA
                     FLDPISQARAAGYQVVQSLIAFSNGGLLGAGVGGGKQKLFYLPEMHTDYIFSVIGEEF
                     GFAGVTLVGACFLTMVWVGYRIARRARDPFGMYLAMGVSSVIGIQALMNMMVGLSMLP
                     PKGMVLPFLSYGGSSLILHLAAVGVLMNISLKGADGLATADDDSRGRDRWARLSGDRP
                     C"
     gene            complement(4122..5468)
                     /gene="murD"
                     /locus_tag="VF357_01330"
     CDS             complement(4122..5468)
                     /gene="murD"
                     /locus_tag="VF357_01330"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX7522816.1"
                     /translation="METFAEKPVIVAGAGRSGLLAARLLVGEGARVTLRDDRSRADVE
                     ASLREPIPAGIAFLRGMPGEGEVRGSALLVVSPGVPRERLPLSALAAAGIPVWGELEL
                     GFRRFQGKVAAITGTNGKSTVTTLVGGMASGAFQRVFVGGNLGTPFVAAAGAPWDWGV
                     VEVSSFQLESIGSFRPRVAALLNLTEDHRDRYAAKEAYYEAKMAVFRNQGPSDTAVVN
                     ADDPEVAARLGSIHARLLPFSVSRALEEGAFLSGREMVLRTASGEERYPRAVLKIPGL
                     QNVENALAAIAIARSMEIPPPAVLAELARFPGLPHRVEFVRSVAGVSYYNDSKGTNVG
                     AVLAALDGFPEPVVLIAGGKDKGVDFRPLRAALGRKARAVVLLGEARDRMARELAGAA
                     PITVTGTLAQAVRAAAEVARTGDAVVFSPACSSFDMFRNFEERGEAFRKAVEELPA"
     gene            complement(5471..6547)
                     /gene="mraY"
                     /locus_tag="VF357_01335"
     CDS             complement(5471..6547)
                     /gene="mraY"
                     /locus_tag="VF357_01335"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX7522817.1"
                     /translation="MLYHLLFPLHESYSFFNVFRYITFRTIYAAITALLISFLLGPWL
                     IRVLRSHQIGQTIRLDGPESHLSKEGTPTMGGLLIVLASVIPTLLWANLANHYIWIAV
                     FVTLGFGAIGFVDDYKKVIRKDTKGLSPHRKLIAQFALAAAAAGLIYMDIGIKDTVII
                     PFLKNVRPSLGFLYIPFIVLVIVGASNAVNLTDGLDGLAIGPSIIAAGTYMLFAYLTG
                     NVKIANYLQIQYVPGVGELTIFCGALAGAGLGFLWFNAYPAQLFMGDTGSLALGGAMG
                     TVAVMVKQELVLVLVGGVFVVEALSVIFQVTSYKTRRKRIFRMAPIHHHFELQGWAEP
                     KIIVRFWIISIILALLAISTLKIR"
     gene            complement(6552..7949)
                     /gene="murF"
                     /locus_tag="VF357_01340"
     CDS             complement(6552..7949)
                     /gene="murF"
                     /locus_tag="VF357_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX7522818.1"
                     /translation="MTLPEVIGAIHPLREAGGISPSEPIGAVTADSRYVGPGSVFIAL
                     PGERADGADFVGEAFGKGALAAIVSEAGARKVPGGLSGTKPVFVVRDPVEALGDMARA
                     HRLRHRDIPLVGITGSSGKTTTKEMLFCLLSRFRRVLRNPGNRNNLIGMPLALLELSG
                     EHDAAILEMGSNVPGELARLAGIACPDIAVITNIAPAHLEGFRTMEGVAREKGDLFRA
                     LGGAGTAVVNATDLRVVREAGRCRAEKIHYGVALNDFSGRIVSMSDAGMRIAVQTPAG
                     EFSSFVGIAGEHHLMNALAATAAAFTLGMRPAEMEEGFAGFVSVPGRFHAVPLRGGGL
                     LLDDSYNANPASTEAALRSLASLARGRRTVVVFGDMLELGESSPASHFRIGHLIAALS
                     VDRLFAFGVAAGHAARGAKEGGMSDAAIDHTTDRGRLREAVRGFVAEGDAVLVKGSRG
                     MRLDEVAADIMEVMA"
     gene            complement(7951..9450)
                     /locus_tag="VF357_01345"
     CDS             complement(7951..9450)
                     /locus_tag="VF357_01345"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX7522819.1"
                     /translation="MKLAELLAGIATVPAGPLGEIGIGGISVDSRSVRPGDLFVAVPG
                     ARADGHLFLADAARGGAAAALVERDLPSPPLPVVRVPSTTAALPGVAVAFHGDPSAKL
                     RVTGVTGTNGKTTVTYLMEAILVAAGRVPAVIGTIDYRVAGTFLRKGLTTPFPHELQA
                     VTAEAVALGATDLLMEVSSHSAAQGRIEGVRFDVGIFTNLTRDHLDFHGDMESYFSAK
                     ARLFREYLPAGGKRTGIAVNGDDPYGERLAHECPSATTFGFSRFWDIHPEEMEMTWEG
                     TRVVLATPAGSLALRSPLIGAHNVSNVMAAVAGGLLLSVSPAAVAAGIAAAGSIPGRL
                     EPIPNARGLHVFVDYAHTPDGMDRVLSTLRGLTGGRLVTVFGCGGNRDRGKRPAMGRV
                     AAMRSDVVVVTSDNPRDEDPALILAEIVPGLTSEGFMEAHGQAAWREGFFRVETDRKA
                     AIALALSLAEPGDTVAIVGKGHEDVQIIGDRRLPFDDRQAVRDVLAAGK"
     gene            complement(9447..>11373)
                     /locus_tag="VF357_01350"
     CDS             complement(9447..>11373)
                     /locus_tag="VF357_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEX7522820.1"
                     /translation="EIRDRGAKQYSSTIPLLPKRGVILDRTGTELAVSIATKSIFVQP
                     AKLHDPNKAADLLAPRVSRSASELRKLFAGEKGFVWVRRQMPSTAAEDAVREVKQALC
                     ALDPEAHGKPSAVDGIGTVEEPKRFYPNRELAASLVGFTNLDSEGMEGIELSLNRYLR
                     GERGSLLCERDARGRLIVPATTPVEVNSKGHSVALTIDRNIQHVAESELQTAVEKYNA
                     RGGMAVVLSPGTGEILAMATAPSFNPNASARAPAETRKNRSLTDSFEPGSTFKVFTLA
                     SALELGAVSTTDRFFCENGSYHYAGKVIHDTHRYGWLTASEVVKFSSNIGISKINDRM
                     DGNQFYDMIRAFGFGSRTGIELQGEVPGLAPSRHGFESRIRRATVSFGQGISVTPLQL
                     AAGMAAVVNGGKVMKPYLVREIRDPEGKTVFRGEPRELRRVLSPKTSAQMREILGKVV
                     EEDGTGTQARIKGFLVGGKTGTAQKVEPGSGRYSATKRTASFIGFLPLNDPKLLILVV
                     IDEPRGQVYGGVVAAPAFNQIAVKTAYYLGIQPTETAALAATRPSGPSTSSRVTPVST
                     ARIAGAMVMPDLSGLSMGRVVDIMGGYSVKLSLAGSGVARVQTPAPGAVLVPGTECSV
                     TFTAEPPMKIAAGERAR"
BASE COUNT         1942 a         4108 c         3629 g         1694 t
ORIGIN      
        1 cgcctcccgg tacccgaaac ggatcgcccg cgcttcgacc cggtggatct ccccctcaac
       61 gtcgaccact tccacccact ccaccaactc cccgatgctc tgcccgtagg ccccggcgtt
      121 catcgacagc gcgccgccca ccgttcccgg gatcccggag agcgactcca tccccgaaag
      181 tccggacagg gcacagagca cggccaaccg cgggagcatc gctcccgcct cgacgaccac
      241 cgccccgtcg gagaggaaca ccaccttgga gaggtgcttc ttcgtgcaca gcacggtggc
      301 cgacacgccc ctgtccgcca ccaggaggtt ggatcccgcc cccaggacat ggacggtgcg
      361 acccgaacgg ccctccgcga cgacggcctc ccggacctgt gccgcggacc gcggaaacac
      421 cagccgatcg gcggcgcccc cgatcccgat ggtggtgtac tcccgcagct tcacctggaa
      481 tgcaacctcg atgtcccgca caaccgtcct cgcctccctg cccctcccgc cgctaccgcg
      541 ggagaaggct ttcgcccagt ttccagacat cgccggcccc catcgtcaga aagatgtccc
      601 ccggtcggag ggttgcgcga accgcctccc tcgcccccgc ggatttgccc agataccggg
      661 cctcccggtg tccgtaatcc cggaccgccg agcacaaatc ttttccggtg gcgccctcga
      721 tcggctcctc ccccgcggag tacacctcga aaacgagaag cacgtccgcg tcctggaacg
      781 ccgagaggaa ctccttgaac agcgcccggg tgcgcgagta ccggtgcggt tgaaatccga
      841 ggacgatgcg ccggtcgggc cagacctccc gcgccgcggc aagggtcgcc cggacctcca
      901 ccgggtggtg gccgtaatcg tccaccaccg tgacgccgtc gatctctccc ttcacctgga
      961 agcgccgccc caccccggcg tagagggaca ggccctcgcg cacctgctcg aaggggatcc
     1021 ggagttccag ggcgaccgcc gttgccgcca gtgcgttgct tacgttgtgg cgcccgggcg
     1081 cccggaggag gacctcgccg agccgctcgc ccctcgcgaa gacggcgaac cggttcgtca
     1141 tcccagacac gaccacctct tccgccctgt agtccgcgga acgggagaac ccgtaggtga
     1201 cgtaggtctt ctccacggag gggatcaact cctggacatt ggggtggtcg atgcacaaga
     1261 ccgagaaccc gtaaaagggg accttgttga tgaagtggag gaacgtctcc ttgatctgcc
     1321 cgatccccga gtagaaatcg aggtgctccg ggtcgatgtt ggtgaccacc gccaccgtgg
     1381 gcgagagttt caggaacgag ccgtcgctct cgtccgcctc cgcgacaagg aattccccct
     1441 ggcccagctt cgcgttggat ccgaggctgt tcagctttcc acccacgacc gccgtcgggt
     1501 cccagccggc cgtcgcgagc accgtggcga ccatcgaggt cgtcgtggtc ttcccgtgcg
     1561 ttccggcgat cgcgatgccg tacttcatcc gcatcaactc cgccagcatc tcggcgcgcg
     1621 gtatcacggg gatcttccgg cggtgcgcct cgaggacctc ggggttgtcg ggccggaccg
     1681 ccgaggagac gaccaccacg tggccatccg ccgggacatt cgaggcggag tgccccgtcc
     1741 gcaccgccgc cccgagccgc tcgagccgct ccgtcgtgtc ggagcggcgc aggtcggacc
     1801 ccgacacccg gtacccgagg ttcaggagca gttccgcgat cccgctcatc ccgatgccgc
     1861 cgattccaac gaagtggatg gggagtcctt tcctatacat tcggtccgac cccctccccg
     1921 ataacgttcc cggtcagccg cagcgccgcg cggaccgcct cgacggccgc gtcgggccga
     1981 gagaaggaaa ccgccgccgc tcccgcgcgt tcccgcgcgt cccgatcgcc gccgagcttc
     2041 cacagcgtcg accacagcgc ctcctccgtc gcttcgcctt ccggcagcca cgctccggcg
     2101 ccggaagcgc agaactccct cgcgttcccc tcctggtggt cgtccgccgc gaacgggtac
     2161 ggaacgagca cgcacgggcg cccgaagagc gccgcctccg cgatggagag ggcccccccg
     2221 cgcatcagca cggcatgaca gcgcgggaac caatccccga tccggtcggt gaacggaaac
     2281 ggctccaccg ggagggcctc gtccccggcc gatcgcgcca ccgcctcgta ctcccgcgtc
     2341 cccgtctgca gcaggaaccg gatcgccgtc ccctcccgct tcgctctccg ggccatcccc
     2401 aacacccgcg tgttgatcgc ccgcgccccc tgcgagcctc ccagcgccag caccgtgaac
     2461 ggcgtctccg gcggaaacga acgcccgcgt tcccgcgccg ccgcgacgat ctccgggcgg
     2521 accgggttcc ccgtgacctc gacccgtccc ggcgggaaga aagggaccgc gcccggaaag
     2581 cccgcgaaca cccggaccgc acgccgcgcc aggaaccggt tcgaacggcc gggcacgctg
     2641 ttctgctcct gcaggaacag cgggatcttc aggagcgcgg cggaaagcgc gaccggcacc
     2701 gaggcgtatc cccccacgcc gaagaccagg tccgggcggc gccgcctcaa gaccgccacc
     2761 cccgcaaaaa acccgagcgc catccggagg gcgcccggga gggccccgat ccccttccct
     2821 cgaacctggc cggaggggac gaagtcgatc gggaaccccc tggccggaac ggcccacgcc
     2881 tcgagacctt cccttgtccc gacgaaggag actgcgccgt cgggacaaag ggagagaaac
     2941 gcatcagcaa gggcgatccc cggaaagacg tgcccaccgg tccctccccc ggctatcatc
     3001 atccgcagtg gcaaggccat ccgccccctt cagggatatg ttcatcagaa cgccgaccgc
     3061 cgccaggtgc aggatcaggg aacttccccc gtagctcagg aacggcagca ccatcccctt
     3121 cggggggagc atcgatagcc ccaccatcat gttcatcagc gcctggatgc cgatcacgga
     3181 ggaaactccc atggcgaggt acatgccgaa cgggtcccgc gcccggcgcg cgatccggta
     3241 ccccacccac accatggtga ggaagcatgc gccgaccagt gtcacgccgg caaacccgaa
     3301 ctcctccccg atcaccgaaa aaatgtagtc ggtgtgcatc tccggcaggt agaacagctt
     3361 ctgcttcccc ccgccgacgc ccgccccgag gagacccccg ttggaaaagg cgatcaggga
     3421 ctgcacgacc tggtaccccg ccgcccgggc ctgcgagatc gggtcgagga acgcctgtat
     3481 tcgcgcgacc cggtacggct tggcaaggat gaggagagcg ccgcccatga cgcccgcggc
     3541 ggcgaaggcg gccaggagtt tccaggggaa cccggcgatg aacacgatca cgaggaacga
     3601 cgcgatgatc acgacggcca tcccgaagtc gggctctttc aggatcaccg cgacgaagag
     3661 ccccatcacc gccagcatcg ggaagaacgc gcgatactca ttcttcggtt tctccccgtt
     3721 ccggtcgatg gcccacgcgg cgaaggcaag cagggcgaac ttggcgaact cggagggctg
     3781 gaaggtgaag agacgcaggt tgatccaccg ggaagcgccg ttcgccgtgt gccggacccc
     3841 ggggacgtac accaacagca gaagcaggaa ggccaccccg acgatccacc cgatccgccg
     3901 gcggtagaag tcgtggtcga cgcgcgacag gcccatggcg aacgcgatcc cgatcgccag
     3961 gaacaggacc tggcgcttga agaagtacgc ggggtcgaac ccgtaccggg ccgacgaacc
     4021 cgccatgacg ttcgttgcgc tgtagatcat gatcatgccg agaaccgtga gcgagagggc
     4081 gcacagcgcg aggatcaggt tctcgtggcg tttcccgagg atcatgcggg cagctcttcc
     4141 acggccttgc ggaacgcctc tccgcgctcc tcgaagttcc ggaacatgtc gaagctcgag
     4201 cacgccggcg agaagacgac cgcgtccccc gtcctggcga cttcggccgc ggcgcgcact
     4261 gcctgggcga gcgtgccggt cacggtgatc ggcgccgccc ccgccagctc cctcgccatc
     4321 ctgtccctgg cctccccaag aagcacgacg gcccgggcct tcctgccgag ggcggcccgc
     4381 aggggccgaa agtcgactcc cttgtccttt cctcccgcga tcagcaccac cggctcgggg
     4441 aatccgtcga gggcggcgag gacggcgccc acgttcgtcc ccttggaatc gttgtaatac
     4501 gacactcccg cgaccgatcg gacgaactcc acccgatgcg gcagtcccgg gaatcgcgcg
     4561 agctccgcca gcacggccgg cggaggaatc tccatcgaac gggcgattgc gatcgccgcg
     4621 agggcgttct ccacgttctg cagcccgggg atcttaagga ccgcccgggg atagcgctcc
     4681 tctcccgagg ccgtccggag caccatctcc ctgccggaca ggaaggcgcc ttcctccagg
     4741 gccctcgaca cggagaacgg gaggagccgc gcatggatcg agcccaggcg cgcggccact
     4801 tcggggtcgt cggcattgac gaccgcagtg tcggacggac cctggttccg gaagacagcc
     4861 atcttggcct cgtaatacgc ctccttcgcc gcataccggt cacgatggtc ctctgtgaga
     4921 ttcagcagcg ccgccacccg gggccggaaa cttccgatcg actcgagctg gaagctggag
     4981 acttccacca cgccccagtc ccagggagcc cccgcggcgg cgacgaacgg cgtcccgaga
     5041 ttccctccga cgaacacccg ctggaaggcc ccggaagcca tccccccgac gagggtggtc
     5101 accgtcgact tcccgttggt tcccgtgatc gccgccacct tgccctgaaa cctccggaag
     5161 ccgagctcga gctcccccca taccgggatc cccgccgcgg cgagggccga gaggggcagc
     5221 ctctcccggg gaacgccggg cgacacgacg agcaaggcgc tcccccgaac ctccccttcc
     5281 ccgggcatcc ctcgcaggaa ggcgatcccg gcggggatcg gctcgcgcag cgacgcctcg
     5341 acgtccgcgc gggagcggtc gtcccgcagc gtgacccggg ctccctcgcc gaccagcagg
     5401 cgcgcagcca ggagcccaga ccgtccggcc cccgccacga tcaccggttt ctcggcgaac
     5461 gtttccatgc tcaccggatc ttcagcgtgc tgatcgccag cagcgcgagg atgatcgaga
     5521 tgatccagaa ccggacgatg atcttcggct cggcccaccc ctgcagttcg aagtggtggt
     5581 ggatcggcgc catccggaag atccgtttcc tccgggtctt gtaggaggtc acctggaaga
     5641 tcaccgagag ggcctccact acgaagaccc cccccaccag cacgaggaca agttcctgct
     5701 ttaccatcac cgccaccgtc cccattgcgc cccccagggc gagggaaccc gtgtccccca
     5761 tgaacagctg cgccgggtac gcgttgaacc agaggaaccc gagccccgcc cccgcgagag
     5821 cgccgcagaa gatcgtcaac tctccgacgc ccgggacgta ctggatctgg aggtagttcg
     5881 cgatcttgac gttgccggtg aggtaggcga agagcatgta cgtcccggcg gcgatgatgg
     5941 aggggccgat cgccagaccg tccagcccgt cggtcaggtt caccgcgttg gaggccccca
     6001 cgatcaccag gacgatgaac gggatgtaga gaaacccgag ggaggggcgg acgttcttaa
     6061 ggaaggggat gatcaccgtg tccttaatgc cgatgtccat gtagatgagc ccggcggcgg
     6121 cggcggcaag ggcaaactgg gcgatcagtt tcctgtgcgg gctcagccct ttcgtgtcct
     6181 tccggatgac cttcttgtag tcgtcgacga aaccgatcgc gccgaacccg agcgtcacga
     6241 acacggcgat ccagatgtag tggttcgcaa ggttcgccca taacagcgta ggaatgaccg
     6301 aggccaggac gatcagcaac ccccccatcg tcggcgtccc ctccttggag aggtggctct
     6361 ccgggccgtc cagccggatg gtctgcccga tctggtgact tcggagaacg cggatcagcc
     6421 aggggccgag gagaaaggag atcagcagcg cagtgatcgc ggcgtagatc gtccggaacg
     6481 tgatgtagcg gaacacgttg aagaacgaat agctctcatg gagcggaaat aggaggtgat
     6541 atagcattct cttacgccat gacctccatg atgtctgccg ccacctcgtc gaggcgcatt
     6601 ccccgcgatc ccttcaccag gacggcgtcc ccttccgcga caaatccgcg caccgcctcc
     6661 cggagccgcc cgcggtcggt cgtgtgatcg atcgcggcgt cgctcatccc gccctccttc
     6721 gccccgcgcg cggcatgacc cgcggcgacg ccgaacgcga agagccggtc gacggagaga
     6781 gcggcgatca ggtgaccgat gcggaaatgc gacgcgggag aactctctcc gagttccagc
     6841 atgtcgccga acacgacgac ggtccgtcga ccccgggcca gcgacgcgag ggagcgcagc
     6901 gcggcctctg tggatgcggg attggcgtta tagctgtcgt ccagcaggag cccgccgccc
     6961 cgaaggggca cggcgtgaaa ccggccgggc acggagacga agcccgcaaa cccttcctcc
     7021 atctcagcgg ggcgcatccc gagcgtgaag gcggcggccg tcgccgcgag ggcgttcatc
     7081 aggtggtgct ccccggcgat cccgacgaag gatgagaact cgcccgccgg cgtttgaacg
     7141 gcgattcgca tccccgcgtc ggacatcgag acgatccgtc cggagaagtc gttcagcgcg
     7201 acgccataat ggatcttttc tgcgcggcaa cggcccgcct cgcggacaac gcggaggtcg
     7261 gtcgcgttga ccacggcggt accggcgccg ccaagggccc ggaagaggtc ccccttctcg
     7321 cgcgcgaccc cctccatggt gcgaaaccct tcgagatgcg cgggggcgat gttcgtgatc
     7381 accgcgatgt cggggcaggc gatgccggcc agccgggcaa gttcccccgg gacgttggat
     7441 cccatctcga ggatcgccgc gtcgtgctcc ccggacagct cgagcagcgc caggggcatc
     7501 ccgatcaggt tgttccggtt tccgggattg cgcaacacgc ggcggaaccg cgagaggagg
     7561 cagaacagca tctccttcgt ggtcgtcttc ccggaacttc cggtgattcc caccaggggg
     7621 atgtcccggt ggcgaagccg atgggcccgg gccatgtcgc cgagggcttc cacggggtcc
     7681 cggacgacga ataccggctt cgttccggac agtccgcccg gcaccttccg cgcgcccgcc
     7741 tccgagacga tcgcggcgag cgcgcctttc ccgaacgcct caccgacaaa gtccgcgccg
     7801 tcggcgcgct cccccgggag cgcaatgaag acggaacccg gcccgacgta acggctgtcg
     7861 gcggtgaccg ccccgatcgg ttccgacggg gagatccccc ccgcctcccg caacgggtgg
     7921 atcgccccga tcacttccgg gagcgtcatt ctacttcccc gctgcaagca cgtcccggac
     7981 cgcttgccgg tcgtcgaacg gcaggcgccg atccccgatg atctggacat cctcatgtcc
     8041 tttcccgacg atcgccacag tgtcccccgg ctccgcgaga gagagggcga gagcgatcgc
     8101 cgctttcctg tcggtctcca cgcggaagaa tccctcccgc caggcggcct gtccatgcgc
     8161 ctccatgaac ccctccgacg tcaacccggg gacgatctcc gcgaggatca aagcagggtc
     8221 ttcgtcccgc gggttgtcgg aggtcaccac cacgacgtcg gaccgcatcg ccgccacccg
     8281 gcccatcgcg gggcgcttcc cgcggtcgcg gtttcccccg cagccgaaca cggtgacgag
     8341 ccgccccccc gtcaaacccc gcaaggtcga gagaacccgg tccatcccgt ccggggtgtg
     8401 ggcgtagtcc acgaacacat ggagcccacg cgcgttcggt atcggctcca gccggcccgg
     8461 gatcgatccg gcggcggcga taccggcggc aactgccgcg ggggacacgg agaggaggag
     8521 acctcctgcg acggccgcca tcacgttgga cacgttatgc gccccgatca ggggcgatcg
     8581 cagcgccagg gaaccggccg gcgtggcgag gaccacacgg gtcccttccc acgtcatctc
     8641 catctcctcg gggtgaatgt cccagaagcg ggagaacccg aacgtcgtcg cggagggaca
     8701 ctcatgcgcg agacgctcgc cgtacgggtc atctccattc accgcgatcc cggtgcgttt
     8761 tccgccggcg gggaggtatt cccggaagag tcgcgccttg gcggagaagt acgattccat
     8821 gtcgccgtgg aagtcgagat ggtccctcgt gaggttggtg aagatgccca cgtcgaaccg
     8881 gaccccctcg atccgtcctt gcgcggcgct gtgggaagag acctccatca gcagatcagt
     8941 ggccccgagg gcgacggcct cggccgtcac cgcctggagt tcgtggggga acggcgtggt
     9001 caaccccttc cggaggaacg tgccggccac acggtaatcg atcgtaccga tcaccgcggg
     9061 aacgcggcct gccgccacca ggatcgcctc catcaggtac gtgaccgtcg tcttcccgtt
     9121 ggtccccgtg acgcccgtca cccgcagctt cgcggacgga tcgccgtgga acgccacggc
     9181 cacccccggc agcgccgccg tcgtggaggg aacgcgcacg accggcaggg ggggggaggg
     9241 aagatcgcgc tccaccaggg ccgccgccgc gccgccgcgc gccgcgtcgg cgagaaacag
     9301 gtggccgtcg gcgcgggcgc cgggaacggc aacaaacagg tcgccgggcc gcacggagcg
     9361 cgaatcgacg ctgatccctc cgatcccgat ctcccccagg gggccggcgg gcacggtggc
     9421 gatccccgcc aggagttcgg ccagtttcac cgcgccctct cgccggcggc gatcttcatc
     9481 ggcggctccg ccgtgaacgt cacggagcac tccgtcccgg ggacgaggac ggctccggga
     9541 gccggtgtct gcactcgggc gactccgctt ccggcgagcg agagtttgac cgaataccct
     9601 cccatgatgt cgacgaccct tcccatcgac agcccagaga ggtcgggcat gaccatcgcg
     9661 cccgcgatcc gcgccgtcga caccggcgtc acacggctcg acgtcgacgg tcccgaagga
     9721 cgggtggcgg cgagcgccgc ggtctccgtc ggctggatgc cgagatagta cgccgtcttg
     9781 accgcgatct ggttgaaggc cggggcggcg acgacaccgc cgtatacctg ccctcgcggt
     9841 tcgtcgatca cgaccaggat gagcagcttc ggatcgttca gcgggaggaa gccgatgaaa
     9901 gaagcagtcc gcttcgtcgc ggagtaccgg cccgacccgg gctcgacctt ctgcgccgtc
     9961 cccgttttcc ctccgacgag gaatcccttg atgcgcgcct gcgttcccgt cccgtcctct
    10021 tcgaccacct tcccgaggat ctcgcgcatc tgcgccgagg tcttcgggga gagaacccgg
    10081 cgaagttccc gcggctcacc gcggaacacg gtcttcccct ccgggtcgcg gatctcccgg
    10141 acgaggtacg gtttcatcac ttttccgccg tttaccacgg ccgccatccc ggcagccagt
    10201 tgcagcggcg tgaccgagat cccctggccg aaggagacgg tggcgcggcg gatgcggctt
    10261 tcgaagccat ggcgggacgg cgcgagcccc gggacctccc cctggagctc gatccccgtg
    10321 cgggacccga agccgaaggc ccggatcatg tcgtagaact ggttcccgtc catgcgatcg
    10381 ttgatcttcg aaatgccgat gttgctggag aacttgacca cctcggacgc ggtgagccac
    10441 ccgtatcgat gcgtgtcgtg gatcaccttc ccggcgtagt ggtaggatcc gttctcgcag
    10501 aagaaccggt cagtggtgct caccgctccc agctcgaggg ccgacgcaag ggtgaacacc
    10561 ttgaaggtcg atccgggctc gaagctgtcg gtcagggaac ggttcttccg cgtctccgcg
    10621 ggtgcgcggg cagaagcgtt cgggttgaac gatggcgccg tcgccatcgc gaggatctct
    10681 cccgttccgg gcgagagcac cacggccatg ccgccgcgtg cgttgtactt ctccaccgcc
    10741 gtctggagct cgctctccgc gacgtgctgg atgttccggt cgatcgtgag ggcgaccgag
    10801 tgccccttcg aattgacctc cacgggcgtc gtcgcgggta cgatgaggcg gccccgggcg
    10861 tcccgctcgc agaggaggga cccccgctcg ccgcgcaggt acctgttcag ggagagctcg
    10921 attccctcca tcccctcgct gtccaggttg gtgaacccga cgagggacgc ggccagttcc
    10981 cggttcgggt agaaccgttt cggctcctcg acggtcccga tgccgtcgac cgccgacggc
    11041 ttcccatggg cttcggggtc gagagcgcag agcgcctgct tcacttccct caccgcatcc
    11101 tccgcggcag tggagggcat ctgccgtcgc acccacacga accccttctc accggcgaac
    11161 agcttccgga gctcgctggc ggaacgggat acccgggggg cgaggaggtc ggccgccttg
    11221 ttgggatcgt ggagcttcgc cggctggacg aagatcgatt tcgtggcgat gctcaccgca
    11281 agctcggttc ccgtccggtc caggatcaca ccccgcttgg ggaggagggg gatggtggag
    11341 ctgtactgct tcgcgccgcg gtcacggatc tcc
//
LOCUS       DASVJL010000074         3433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1511400, whole genome shotgun sequence.
ACCESSION   DASVJL010000074 DASVJL010000000
VERSION     DASVJL010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511400"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VF357_01355"
     CDS             complement(<1..1238)
                     /locus_tag="VF357_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7522821.1"
                     /translation="MFLSDLSIKRPIFASVMMLALVTLGAFSYKRLAIDMFPDVEIPV
                     ITIVTKFPGASPESVEREVSKRIEEAVNPIAGVKKVISISRESVSTVMVQFQLEVKLN
                     DGAQEARAKIAAIRGELPEGIEEPIIQKLDFAAAPIVSLAVRSDTLPPKDLTTLVEKK
                     VKRRFENIPGVGKVDLVGSSKREVNVDIDQARLEALGMGVDEVIAGIRSENVNTPLGR
                     LTKNGSEFPLRVSGKPAEVSGFPSMVIGQRNGRPVLLGEVADVVDGIEEPRSLALVNG
                     VPAIALDIQKQSKANTVSVVELVKKEIARLQPELPPGTRIEIVRDASTMIRDSVEDVQ
                     TTLVLGGILTIFIVFCFLNSWRSTVITGLTLPISVISSFIVMNFMGMTLNVMTLMALS
                     LAIGLLIDDAIVVRENIVRHL"
     gene            complement(1242..2396)
                     /locus_tag="VF357_01360"
     CDS             complement(1242..2396)
                     /locus_tag="VF357_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7522822.1"
                     /translation="MNRRFALGAAVLAAFLAAAGCSSKEGIEGAKAASAAKPAVSVDA
                     AKAATGDVTEGIEVTGTLSPKYQAEMKSEYGGVVAQVYVNDWAKVRKGDPLLKVDTRE
                     GEVLLLKARAAHQMAKAGQLEAEAAVARADREYDRAIKLQESGLLTRQGMDDARTQKE
                     AAAARIAAAKAQVAAAGEDVAQATTRLSKAVIRSPFDGTVAERLVSPGDLVGEMQKVV
                     FRLVDNRLLELTVSVPSTEMAALRVGQPVRFSSDAFPGREFDGKVAHINPSVSPGDRS
                     VRVIAEVPNDPEVLKGGLFVKGRIVTGGRKGVVQVPRTALLSWNVAARKGEVFVVDNN
                     VARRRGVTTGAVQGDRVEIPSGLRPGETVVIRGAFLLSDGDAVKVAAAGK"
     gene            complement(2393..3034)
                     /locus_tag="VF357_01365"
     CDS             complement(2393..3034)
                     /locus_tag="VF357_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7522823.1"
                     /translation="MAKRERKEAEAGGEVKERLLSGATGLFASKGYAATTVREIVERA
                     GVTKPVLYYYFRSKEGIYLDLMREPFSKFAALVEETLVPAGSARERLFRLCLMGYDIF
                     VENLDAARVMYSIYYGPPQGAPFIDFDAYHRRFQEAVLQVLREGILDGEFRRVNLQDA
                     MWAVTGAVNVAMEVELCHPSQSLGRDGVRRVLEIVLEGVATDRNVNGEKERKR"
     gene            complement(3179..>3433)
                     /locus_tag="VF357_01370"
     CDS             complement(3179..>3433)
                     /locus_tag="VF357_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEX7522824.1"
                     /translation="AMSPHMEPGEGSVGTLVNGTHTAATPPGMTVTVRVRCVGVEGRR
                     TVWDITAADNVEVIGKGTHERFAVDFAKFNARVAKKAAGV"
BASE COUNT          553 a         1251 c         1049 g          580 t
ORIGIN      
        1 aggtgccgga cgatgttctc ccggaccacg atcgcgtcgt cgatgaggag gccgatggcg
       61 agggacagcg ccatcagcgt catcacgttg agcgtcattc ccatgaagtt catgacgatg
      121 aaggaggaga tgacggagat cgggagggtg agtccggtga tcaccgtgga gcgccacgag
      181 ttgaggaagc agaagacgat gaagatggtc aggatcccgc cgaggaccag ggtcgtctgg
      241 acgtcctcca cggagtcccg gatcatggtc gaggcgtccc ggacgatctc gatcctcgtc
      301 ccggggggaa gctccggctg cagccgggcg atctccttct tcaccagctc gaccacggag
      361 accgtgttcg ccttcgactg cttctggatg tcgagcgcga tcgcggggac gccgttcacc
      421 agcgcaagag accgcggctc ctcgatcccg tcgaccacgt cggccacctc gcccagcaac
      481 acgggccgcc cgttcctctg accgatcacc atcgacggga atccggacac ctccgcgggc
      541 ttccccgaaa cgcgcaaagg gaattcggac ccgttcttcg tcaaccgccc aagcggggtg
      601 ttgacgtttt cggaccggat ccccgcgatc acctcgtcca cgcccatccc gagcgcctcg
      661 aggcgcgcct ggtcgatgtc gacgttgacc tcccgtttcg acgaccccac gaggtcgacc
      721 ttcccgacgc cggggatgtt ctcgaagcgc cgcttgacct ttttctccac caaggtggtg
      781 aggtccttcg gcgggagggt gtcggaccgg accgccaggg atacgatcgg cgccgccgcg
      841 aagtccagct tctggatgat cggctcctcg atcccctccg gcagctcgcc gcggatggcc
      901 gcgatcttgg cccgcgcctc ctgggcgccg tcgttgagct tcacctcgag ctggaactgg
      961 accatgaccg tggagacgct ctcgcgggaa atcgagatga ctttcttcac gccggcgatc
     1021 gggttgaccg cctcctcgat ccgcttgctg acctcgcgct cgacgctctc cggagaggcg
     1081 ccggggaatt tcgtgacgat ggtgatgacg gggatctcca cgtcggggaa catgtcgatc
     1141 gcgagccgct tgtaggaaaa ggcgcccagc gtcacaaggg ccagcatcat gaccgaggcg
     1201 aagatggggc gcttgatcga gaggtcggag agaaacatcg cctatttccc cgccgcggcg
     1261 accttcaccg catcgccgtc ggaaaggagg aacgccccgc ggatgaccac cgtttcgccg
     1321 ggccgcaggc ccgaggggat ctcgacccgg tccccctgga ccgctcccgt cgtcaccccc
     1381 cggcggcggg cgacgttgtt gtcgacgacg aagacctccc ccttccgggc ggccacgttc
     1441 caggacagaa gggccgtccg cgggacctgg acgaccccct tccggccgcc ggtcacgatc
     1501 ctccccttca cgaacaatcc gcccttcagg acctccggat cgttgggcac ctcggcgatc
     1561 acgcgaacgg agcggtcacc ggggctcacc gacgggttga tgtgggcgac ctttccgtcg
     1621 aactccctgc cggggaacgc atccgaggaa aaccgcaccg gctgccccac gcgcagggcg
     1681 gccatctccg tggaaggcac gctcacggtc agttcgagga gccggttgtc cacgaggcgg
     1741 aagaccactt tctgcatctc gcccacgagg tcccccgggc tcaccaaccg ctccgccacc
     1801 gtcccgtcga agggggagcg gatcaccgcc ttggaaaggc gcgtcgtcgc ctgggcgacg
     1861 tcctcccccg cggccgccac ctgggctttg gccgccgcga tccgcgcggc cgccgcctcc
     1921 ttttgcgtcc gggcgtcgtc catcccctgc cgcgtgagca gcccggactc ctgcagcttg
     1981 atcgcccggt cgtactcccg gtcggcgcgc gcgacggccg cctcggcctc cagctgcccg
     2041 gccttcgcca tctggtgcgc cgcccgcgcc ttcaggagga gaacctcccc ctccctcgta
     2101 tccaccttga gcaggggatc ccccttgcgc accttcgccc agtcgttcac gtagacctgc
     2161 gccaccacgc cgccgtactc cgacttcatc tccgcctgat acttcggcga caaggtcccg
     2221 gtgacctcga tcccctcggt gacatccccc gtcgcggcct tcgccgcgtc cacggaaacg
     2281 gcgggcttcg ccgccgacgc ggccttcgcc ccctcaatcc cctccttcga agagcagccg
     2341 gccgccgcga gaaacgcggc gagaacggcg gctccgaggg cgaatctccg gttcatctct
     2401 tcctctcctt ttctccattg acgttccggt ccgtggcgac gccttcgaga acgatctcca
     2461 ggacgcgccg tacgccgtcc cgcccgagac tctgcgacgg gtggcacagc tccacctcca
     2521 tcgccacgtt gaccgcaccg gtgacggccc acatcgcatc ctgcaaattc acccgccgaa
     2581 actcgccgtc caagattcct tcccggagga cctggagcac cgcctcctgg aaacggcggt
     2641 ggtaggcgtc gaagtcgatg aacggcgcgc cctgcggggg cccgtagtag atggagtaca
     2701 tcactctcgc ggcgtcgagg ttctcgacga agatgtcata ccccatgagg cagagccgga
     2761 acagccgctc ccgcgccgag ccggccggca cgagcgtctc ctcgacgagc gcggcgaact
     2821 tgctgaaggg ctcccgcatc aggtcgaggt agatcccctc cttgctgcgg aagtagtagt
     2881 agagtaccgg cttggtcacg cccgcccgct cgacgatctc gcggacggtc gtggccgcgt
     2941 accctttcga ggcgaacagg ccggtcgccc cggacaggag gcgctccttc acctcgccgc
     3001 ccgcctcggc ctctttccgc tcccttttcg ccataccgcc ctcccgccgc cttgcggtcc
     3061 aatatttacc taccggtaag tatacgggcg ggcgcaaccg tgtcaacgaa aaatttaccg
     3121 atcggtaaat aaaaatggcg gttcccacgg gcggatttcc cgggggaagg aaggatcttt
     3181 acacccccgc ggccttcttc gccacgcggg cgttgaactt cgcgaagtcg acggcgaacc
     3241 gctcgtgggt ccccttgccg atgacctcga cgttgtccgc agccgtgatg tcccaaaccg
     3301 tccggcgccc ctccaccccg acgcaccgga cgcggaccgt gacggtcatc ccgggcgggg
     3361 tcgccgcggt gtgcgtaccg ttgacgagcg tcccgacgct cccctccccc ggctccatgt
     3421 gcggcgacat cgc
//
LOCUS       DASVJL010000075         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_292573, whole genome shotgun sequence.
ACCESSION   DASVJL010000075 DASVJL010000000
VERSION     DASVJL010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292573"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VF357_01375"
     CDS             complement(<1..314)
                     /locus_tag="VF357_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522825.1"
                     /translation="MLSATDRSAVFPPEMTADRRRFLALLREKSYERRKVVLSSGRES
                     DFYIDCRQSTLDAEGAILTGRLFCAMLERGERPEAVGGITLGADPIVTAVSLTSSLRG
                     WPV"
     gene            complement(357..1778)
                     /gene="purF"
                     /locus_tag="VF357_01380"
     CDS             complement(357..1778)
                     /gene="purF"
                     /locus_tag="VF357_01380"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX7522826.1"
                     /translation="MSDGWHDECGIFGIHGHPEASNMAYLGLYALQHRGQESAGIAST
                     DGEKIIFHKEMGLVADIFSEEILSRLPGHSAIGHVRYSTTGSSELKNAQPLVVDFESG
                     AIAIAHNGNLVNAQELKRELEVSGSIFQSSMDTEVIVHLIARSRKERIEDRIIDALNQ
                     VRGAYSLLFMTRDKLIGVRDPHGIRPLALGKMRGEGGGTVITSESCALDLIEAEYLRE
                     VEPGEMIVVDVRGTHSHRPFLPAIERFCIFEFVYFARPDSIMGGTCIYEVRKALGRQL
                     AKECPANADVVIPVPDSGVPAALGFSEASGIPFEMGLIRNHYVGRTFIEPQQSIRHFG
                     VKIKLNAVRGVVSGKRVVVVDDSIVRGTTGRKIIKMIRAAGAKEVHFRISSPPTCYPC
                     FYGIDTPERRDLIAATHTREETNTYLTSDSIGYLSLEGLHACVPKGNATYCDACFSGN
                     YSVPLGTEESGEQLPLFRGSVRV"
     gene            complement(1789..>3246)
                     /locus_tag="VF357_01385"
     CDS             complement(1789..>3246)
                     /locus_tag="VF357_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX7522827.1"
                     /translation="DAIVGIQDMGAAGLTSSSFEMASRGGSGFLMDLDRVPQREEGMT
                     PYELMLSESQERMLIVAKKGREDEVREIFEKWDLDVSVIGEVTGDGIGRVRWRGKTVA
                     EIPIAALTDKAPVYDRPAERPEWQDALQRLDPAGLPAPSDLGETWVRLMGGPELADKK
                     WVYRQYDHMIRTNTLILPGSDAAVLRVKGTRKGIALSVDCNSRYCYLDPFVGGMIAVC
                     EAARNVACSGAEPIGLSDCLNFGNPEKPEVMWQFRSAIEGMAKACEALGVPVVSGNVS
                     FYNETMGKGIHPTPAVAMVGLLEDASKRRVQWFSTEGDVILLAGADRLDVHLGGSLYL
                     KEVHGKVAGTPPTVDLAHEKRLQEFLREAVSADLVASAHDLAEGGFACALAESCISGP
                     GNAMGAQVLNPFPEATRPDFAMFSECQGAVLLSCAPAKRDTLLDLARRFRITIKELGI
                     VGGEHVDIEGLARVGVAELRTAWSEGFAAALGLEG"
BASE COUNT          520 a         1151 c         1025 g          550 t
ORIGIN      
        1 accggccatc cccgcaggga gctggtgagc gaaaccgcgg tcacgatcgg atccgccccg
       61 agggtgatcc cccccaccgc ctccggtcgc tccccccgct cgagcatcgc gcagaagagc
      121 cgccccgtga ggatcgcccc ctccgcgtcg agcgtgctct gcctgcagtc gatgtagaag
      181 tcggattccc gtcccgagga aaggacgacc ttgcgtcttt cgtagctttt ctccctcagg
      241 agggccagga aacgccgccg gtcggcggtc atctccggcg ggaacacggc agatcgatcg
      301 gtcgcgctga gcatcgtttc ccccggatgt gtgatctctc ggcttttccc ggtccgtcag
      361 acccggaccg agccgcggaa cagcggcaac tgctccccgg actcttccgt tccgagcgga
      421 accgagtagt tccccgagaa gcaggcgtcg cagtaggtcg cattcccttt cggcacgcag
      481 gcgtggaggc cctccaggct gaggtatcca atgctgtcgg aggtgaggta ggtgttcgtc
      541 tcctccctcg tgtgggtcgc ggcgatcagg tcccgccgct ccggagtgtc gattccgtag
      601 aagcacgggt agcacgtcgg gggggagctg atccggaagt ggacctcttt cgccccggcc
      661 gcccggatca tcttgatgat cttgcgcccg gtcgtccccc ggacgatgga gtcgtccacc
      721 accacgaccc gtttccccga gacgactccg cgcaccgcgt tcagcttgat cttcacgccg
      781 aagtggcgga tcgactgctg cggctcgatg aaggtgcgac ccacgtagtg gttccggatg
      841 aggcccatct cgaaggggat cccggacgcc tcggagaacc ccagcgccgc ggggacgccc
      901 gagtcgggca cggggatgac gacatcggcg ttcgccggac actccttcgc cagctgccgc
      961 ccgagagcct tgcgaacctc gtagatgcac gtgccgccca tgatcgagtc cggacgggcg
     1021 aaatagacga attcgaagat gcagaaccgc tcgatcgccg gaagaaacgg ccggtgggag
     1081 tgcgtccccc ggacgtcgac cacgatcatc tcgcccggtt ccacctcccg caggtactcc
     1141 gcctcgatca ggtcgagcgc acacgactcg gaggtgatga ccgtcccccc cccctctccc
     1201 ctcatcttcc cgagggccag cggccggatc ccgtgggggt cgcgcacccc gatcagcttg
     1261 tcccgcgtca tgaagaggag ggaatacgcg ccgcggacct ggttcagcgc gtcgatgatg
     1321 cgatcctcga tccgctcctt cctggaacgg gcgatgaggt gcacgatgac ctcggtgtcc
     1381 atcgaggact ggaagatgga gccggagacc tcgagctccc gcttgagttc ctgggcgttc
     1441 acgagattgc cgttgtgggc gatggcgatg gcgccggact cgaagtcgac caccagcggc
     1501 tgggcgtttt tcagctccga gctgcccgtc gtggagtacc ggacgtgccc gatcgccgag
     1561 tgccccggca gccgggaaag gatctcctcg gagaagatgt cggcgaccag ccccatctcc
     1621 ttgtggaaga tgatcttttc gccgtcggtg ctggcgatcc ccgcgctctc ctggccccgg
     1681 tgctgcagcg cgtagaggcc caggtacgcc atgttggaag cttcgggatg cccgtggatc
     1741 ccgaagatgc cgcactcgtc gtgccagccg tcggacattc gcgatcccct atccttccag
     1801 gcccagggcc gccgcgaacc cctcgctcca cgccgtccgg agttccgcga cccctaccct
     1861 cgccaagcct tcgatgtcga cgtgctcccc gccgacgata cccagttcct ttattgtaat
     1921 acggaagcgc cgggcgagat cgaggagggt gtcccgtttc gccggcgcgc aggagagcag
     1981 gacggccccc tggcactccg agaacatggc gaaatccggc cgggtcgcct ccgggaacgg
     2041 gttgaggacc tgcgcgccca tcgcattccc ggggcccgat atgcacgact ccgcgagggc
     2101 gcaggcgaag cctccctccg cgaggtcgtg cgccgaggcg acgaggtcgg ccgagacggc
     2161 ctcccgcagg aactcctgga gtcgcttctc gtgggcgagg tcgaccgtcg gcggggttcc
     2221 ggccaccttc ccgtggactt ccttgaggta gagggaaccg cccaggtgaa cgtcgagccg
     2281 atccgccccc gccaggagga tgacgtcccc ctcggtggaa aaccattgga cccgccgctt
     2341 cgacgcgtcc tcgagcagcc cgaccatcgc caccgcggga gtggggtgga tcccctttcc
     2401 catcgtctcg ttgtagaagg agacgtttcc ggaaaccacc gggacgccga gggcctcgca
     2461 cgccttcgcc atcccctcga tcgcggagcg gaactgccac atgacctccg gcttttcggg
     2521 gttgccgaag ttcaggcagt ccgaaagccc gatcggctcc gcgccggagc aggcgacgtt
     2581 gcgggccgcc tcgcacacgg cgatcatccc gccgacgaac ggatccaggt agcagtaccg
     2641 gctgttgcag tcgaccgaca gggcgatccc cttccgcgtc cccttgaccc gcagcacggc
     2701 cgcgtccgac ccggggagaa tgagggtatt cgtccggatc atatggtcgt actggcggta
     2761 gacccacttc ttgtcggcca gctcgggacc gcccatcagg cgcacccacg tctccccgag
     2821 gtccgaaggc gccgggagcc ccgcgggatc gagtctctgg agggcgtcct gccactccgg
     2881 gcgctccgcc ggccggtcgt agacgggcgc cttgtccgtc aacgcggcga tcgggatctc
     2941 cgcaaccgtc ttcccgcgcc accgcacccg gccgatcccg tcccccgtga cctctccgat
     3001 caccgagacg tcgaggtccc acttctcgaa gatctcccgg acctcgtcct cccgtccctt
     3061 cttcgccacg atgagcatcc gctcctggga ctccgagagc atcagctcgt agggggtcat
     3121 tccctcctcg cgttgcggca cccggtcgag gtccatcagg aacccggatc ctccccggga
     3181 agccatctcg aacgaggagg aggtgagacc cgctgcaccc atgtcctgga tcccgacgat
     3241 cgcgtc
//
LOCUS       DASVJL010000076         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_386171, whole genome shotgun sequence.
ACCESSION   DASVJL010000076 DASVJL010000000
VERSION     DASVJL010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386171"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VF357_01390"
     CDS             <1..670
                     /locus_tag="VF357_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522828.1"
                     /translation="KWEGMEKMKYDMAGGAGMLATLRAAAALGIRRNLVAVVPCVENM
                     PSGTAYRPGDVLRMMSGKTVEILSTDAEGRLILADAMTYAVRKYRPKEMLDAATLTGA
                     CIVALGSVNIGMMGNDEGMLSRMKAASAASGEKAWELPLHEEYFEQIKSDIGELKNVG
                     GGEAGTITAGRFLQEFAGGTPWVHFDIAGTAWVEKEKRGYAPGPSGAPVRLLVEYFSS
                     RDGH"
     gene            695..1360
                     /locus_tag="VF357_01395"
     CDS             695..1360
                     /locus_tag="VF357_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522829.1"
                     /translation="MTEMPALDPEGRNDISCPHCTIQIPDDVPDCPHCRQPMPGGTSG
                     KPKDIRELLVPPERFPRLRKYYREHGKWVKVVAPALLAVLALWLALGYLTRVRIVVPS
                     DNTFLIEAELERKDGGRVLLTGKLANRGEDIPDLSLRSIGVIAELLYGDGRTERRRVF
                     PKSPFRGEGALFRGESGAFEIEVPKGVKSVTLRGEIVNLGEDRKFDPVTRRIRRLPAQ
                     RSR"
     gene            2231..3118
                     /gene="hisG"
                     /locus_tag="VF357_01400"
     CDS             2231..3118
                     /gene="hisG"
                     /locus_tag="VF357_01400"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX7522830.1"
                     /translation="MKGKKSNILNIGLPKGSLQESTLHLFRKAGFNINVGSRSYVPTI
                     DDPELSGLLIRAQEMARYVQDGILDMGLTGRDWVLEQNAKVKEVCPLLYARGGLRPVR
                     WVVAVPNDSPIQRIRDLQGKRVATELVQFTRRYLKKKGVEAQVEFSWGATEVKAPRLA
                     DAIVELTETGSSLRANNLRVVETILESTTVLIANREAWRDPWKRDKIGNIALLLQGAL
                     RAEEKVGLKMNVGRGDLDRILKVLPAMQNPTISTLSEAGWFSLEVIVDQKIVRDLAPL
                     LKKEGASGIVEYPLNKVIP"
BASE COUNT          655 a         1150 c         1194 g          566 t
ORIGIN      
        1 gaagtgggag gggatggaga agatgaagta cgacatggcg gggggcgcgg ggatgctggc
       61 gacccttcgg gccgccgccg cccttgggat ccggcggaac ctcgtcgccg tcgtcccctg
      121 cgtggagaac atgccctccg gtaccgcgta tcggcccggc gacgtccttc ggatgatgtc
      181 ggggaagacc gtcgagatcc tctcgaccga cgcggagggc cgtctcatcc tggcggatgc
      241 gatgacgtac gccgtccgga aataccggcc gaaggagatg ctcgacgcgg cgacgctcac
      301 gggcgcgtgc atcgtggcgc tgggaagcgt caacatcggc atgatgggga atgacgaggg
      361 gatgctctcc cggatgaagg cggcatccgc ggcgtcggga gagaaggcgt gggagctgcc
      421 gctgcacgag gagtatttcg agcagatcaa aagcgacatc ggggagttga agaacgtcgg
      481 cggcggcgag gccgggacca tcactgccgg ccgcttcctt caggagttcg cgggcggcac
      541 gccgtgggtc cacttcgaca tcgccggcac cgcctgggtg gagaaggaga agcgcgggta
      601 tgctccgggg ccttcgggcg cgccggtccg cctcctcgta gagtacttct cttcccggga
      661 cgggcactga cggcggggct gcgggagaat cgccatgacg gaaatgccgg cgctcgaccc
      721 ggaaggccgg aacgacatca gctgcccgca ctgcacgatc caaatccccg acgacgtccc
      781 cgactgcccg cattgccggc agcccatgcc gggcgggacc tccggaaagc cgaaggacat
      841 ccgcgaactg ctggttcccc cggaacgctt tccgcgcctc cggaaatatt accgggaaca
      901 cggcaagtgg gtgaaggtcg tagcaccggc tctcctcgcg gtccttgccc tgtggctggc
      961 gctcggatac ctgacgcgtg tgaggatcgt cgttccgtcg gacaacacct ttctgatcga
     1021 ggcggagctg gaacggaagg atggcggcag ggtcctcctg accgggaagc tcgccaatcg
     1081 gggggaagac atcccggatc tctccctgcg ctccatcggg gtgatcgcgg agctcctgta
     1141 cggcgacggg aggacggagc ggaggcgcgt tttcccgaag tccccgttcc gcggtgaagg
     1201 ggcgctcttc cgaggggaat cgggagcgtt cgagatcgag gtgccgaagg gggtgaagtc
     1261 ggtcaccctc cgcggcgaga tcgtgaacct cggagaagac cggaagttcg atcctgtgac
     1321 ccggagaatc cgccgcctcc cggcccaaag aagccggtga tccacccgcg ggtacggggg
     1381 cacgcgggcc gcggatgcgt cctcgcccac tccctcagtg caagaaggtc ctccccggtg
     1441 tccacgtccc gcccgggagg caggaacgag aacggcgccg aaaggatcct gcagcgcgcc
     1501 gccgcgttcc ggagcacccc cgcggtgctc catcggaatc cccgaaacag acgttcgtcc
     1561 ggcgaggaga ggccgatcag atagtatcct ccgtccgccg aagggccaaa gacgaacgac
     1621 gctccgttgg aaagctcccg gaatgcccgg cggaccgtcg ccgcggagag tgaagggcag
     1681 tccgcaccca cgatcaccac gcgacgtgcc ccgtgccgga aagcggtcgc cgctccctcc
     1741 cgcatcctgt ccccgaggtc cccgccgcgc tgcggaaacg gctcgaacgc cgaaaaagga
     1801 ggaccccgaa gggagtccac ggactcgggc ggatcgagga agagatatcg ccggacacag
     1861 gtcaatgcgg acatctctgc cgccgtgtcc ccgagcatgc agccgtacaa gcgcaccgat
     1921 tcgcggtggt cgagcggagg gcaaaggcgg gtcttgaccc ggccggcggc cggtgccttc
     1981 gccatcagta cgaccgcttc ctccattgcc ctcccacctt tccattatcg agggtacccc
     2041 agggagcgta tttcgtccgg gagtgaggtg tccgtccgcc tttccattcg cgtcgaccgc
     2101 gtggcatggc ccattctacc ggaaacctac ccgccgcgat ctcatcggca tttccccccc
     2161 cttgcttgtc aggcgaaccc acgatgtgta taatttcggg tttcaccgtt tccacccgaa
     2221 gaggagcgcc atgaaaggga agaagagcaa catcctgaac ataggtctgc cgaaggggag
     2281 cctgcaggag tccaccctgc acctgttccg caaggccggc ttcaacatca acgtcggctc
     2341 ccgcagttac gtccccacga tcgacgaccc ggaactttcc ggtctcctga tccgcgccca
     2401 ggagatggcg cgttatgtcc aggacggcat cctcgacatg ggcctgaccg gtcgggactg
     2461 ggtgctggag cagaacgcga aagtgaagga ggtttgtccc ctcctctatg cgcggggggg
     2521 cttgcgcccg gtccggtggg tggtggcggt cccgaacgac tcccccatcc agcggatccg
     2581 ggatctgcaa ggaaagcggg tggcgaccga actggtccag ttcacccggc gatacctgaa
     2641 gaaaaagggg gtcgaggccc aggtcgagtt ctcctggggg gccaccgagg tgaaggcgcc
     2701 gcgccttgcc gacgcgatcg tcgagttgac cgagacgggg agcagccttc gggcgaacaa
     2761 cctccgcgtc gtggagacga tcctcgagtc gaccaccgtc ctcatcgcaa accgggaggc
     2821 atggagggat ccgtggaagc gagacaagat cgggaacatc gccctgctcc tccagggcgc
     2881 tctccgcgcg gaggagaagg tcggtctcaa gatgaacgtc ggccgtggcg acctcgaccg
     2941 gatcctcaag gtgctgccgg cgatgcagaa cccgacaatc tccaccttga gcgaagccgg
     3001 ctggttctcc ctcgaggtca tcgtcgacca gaaaatcgtc cgggatctgg cccccctcct
     3061 caagaaggaa ggggcttccg ggatcgtcga atacccgctg aacaaggtca tcccttagtg
     3121 cttcgtttca taattactgc aacattccag tgaaagggac tccaggctcg cgggggtgct
     3181 tccactccgc taccatcgtc gatggccagg gatggccaag tgccgcgggc gcatggatgc
     3241 gcaggagcgg ccgaccgtcc atggtctcct cgcctgcgcc tccgctcgtt cccgacgtcc
     3301 ctgtctccac ttcgctccag acgccgcttc gtggaacccc cccgctgcgc cctgcgtccc
     3361 cgcaccccgg aggcgcatcg gagcgggata ccccttccga gcgggcctgt cctcgccgat
     3421 ggccagggat ggccaagtgc cgcgggagcc atggatggcg ggagcggcca gtgcaggcag
     3481 ccagggatgg ctgccggaac gtagcgagga agggggtatc ccgcggagat caaggcgcct
     3541 ccgtttatga tacagtattt atgaa
//
LOCUS       DASVJL010000077         3259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_668054, whole genome shotgun sequence.
ACCESSION   DASVJL010000077 DASVJL010000000
VERSION     DASVJL010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668054"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VF357_01405"
     CDS             complement(<1..481)
                     /locus_tag="VF357_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7522831.1"
                     /translation="MSKRFGKKVVLDGLELTVPKGKNTVVIGGSGTGKSVLIKCAVGL
                     LRPDAGEIRIDGQDIIRMDERELVRVRRRFGMLFQGAALFDSMDVGENVAFVLRRLKM
                     YPEGQIREVVEEKLSMVGLRDIQRLMPAELSGGMKKRVGLARAIASEPDILLYDEPTT
                     "
     gene            complement(521..1321)
                     /locus_tag="VF357_01410"
     CDS             complement(521..1321)
                     /locus_tag="VF357_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7522832.1"
                     /translation="MTESTPKKKGAVQLAELLGERVYAMVLDLGAIFTLFVQVVSGAV
                     RPPSEIHNIVKQMEEVGIRSMPVVLVTATFTGMVLALQSYAGFQRFGATSFVGSVVAL
                     SITRELGPVFAGLMVSGRVGASMAAELGTMKVTEQIDALVTLATNPVKYLVVPRVVAA
                     TIVLPVLVVFADLLGIVGGYFVSVYLMGANPYVYMAKTYQYLEFKDIYTGLVKASVFG
                     MLIALISCHHGFVAQGGAEGVGRATTRAVVASSMMVLISDYFMTSFMF"
     gene            complement(1314..2429)
                     /gene="alr"
                     /locus_tag="VF357_01415"
     CDS             complement(1314..2429)
                     /gene="alr"
                     /locus_tag="VF357_01415"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX7522833.1"
                     /translation="MARPTVAEIRLSALRHNVQVIRALLPQGVGLLGIVKANAYGHGA
                     LSVARALEADGLRMLGVATVEEGVELREGGIRLPVVVLGGVDPPQAAEAHAHGLSAVL
                     FDLGQIGNLARAAEVEGRPFPVHVKVDTGMGRLGLLPREAMEAAERIASTPALRLEGW
                     MTHLSSADGPAAEDREYTAMQLAAFRGMLPAVRKAFGGGVAVHALNSAGILRFREEPF
                     DLVRPGISMYGCSPLPPGDASPDLRPVMRVVTKVMSLKELPAGHAVSYNRRYRCDGAR
                     RIAVVPIGYADGYRRALTGRARMSVAGHAVPVAGTVCMDHTMIDVTGVPGVSIGTDVE
                     VMGEASMGAEEMARVCGTIPYEILVQVGPRIPRVTVD"
     regulatory      2545..2671
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2729..>3259
                     /locus_tag="VF357_01420"
     CDS             2729..>3259
                     /locus_tag="VF357_01420"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX7522834.1"
                     /translation="MTLMYRARQGEIPPAIALAAQSEHVAPEKLRDLVAVGRVVIPRN
                     RKRREIRPVAIGEGLTVKVNANVGSSRDRADISLEMEKMRAAVAAGADAVMDLSTGGP
                     IDEIRRAILAECPVPIGTVPLYQAAADGNLRGKSWVDLTAVDFFAAIEKQAMEGVDFM
                     TLHCGVTRTSVERLVRE"
BASE COUNT          562 a         1165 c         1038 g          494 t
ORIGIN      
        1 ccgtcgtcgg ctcgtcgtac agaaggatat cgggctcgga ggcgatcgcc cgggccagcc
       61 ccacgcgctt cttcatgccg ccggacagct ccgcgggcat cagccgctgg atgtcgcgca
      121 gcccgaccat cgagagcttc tcctccacca cctcccggat ctggccctcc gggtacattt
      181 taaggcggcg caggacgaag gccacgttct cccccacatc catcgaatcg aacagggccg
      241 ccccctggaa aagcatcccg aacctgcggc ggacccggac cagctcccgc tcgtccatcc
      301 gtatgatgtc ctggccgtcg atccggatct cccccgcgtc cgggcgaagc agccccaccg
      361 cgcacttgat gaggaccgac ttgcccgtgc cgcttccgcc gatgacgacg gtgttcttcc
      421 ccttcggcac ggtcagctcg agaccgtcga ggaccacctt ctttccgaag cgtttactca
      481 attcccggat cgcgatcacg ctggcttcgg ccctttcccg tcagaacatg aacgatgtca
      541 tgaagtaatc cgagatgagc accatcatgg aggacgccac gacagcccgt gtggtcgccc
      601 ggccgacccc ttccgcgccc ccctgcgcca cgaatccgtg gtggcacgag atgagggcga
      661 tcagcatacc gaagaccgat gccttgacca gccccgtgta gatgtccttg aattcgaggt
      721 actggtacgt cttggccatg tacacgtacg ggttggctcc catcaggtaa acggagacga
      781 agtagccgcc gacgatcccc agcagatccg cgaacacgac gagcaccggg aggacgatcg
      841 tcgcggcgac cacgcgcggc accacgaggt acttgaccgg gttggtggcg agtgtgacca
      901 gggcgtcgat ctgctccgtc accttcatcg tccccagctc cgccgccatc gaggcgccga
      961 cgcggccgga gaccatgagg ccggcaaaca ccggccccag ttcccgcgtg atggaaaggg
     1021 cgacgaccga cccgacgaag ctggtcgcgc cgaaccgctg gaacccggcg tagctctgca
     1081 gcgcgaggac catgccggtg aaggtggcgg tgacgagcac aacgggcatc gaccggatcc
     1141 cgacctcttc catctgcttg acgatgttgt ggatctccga cggcggccgc accgccccgg
     1201 acacgacctg gacgaacagg gtgaagatcg cccccaggtc cagtaccatg gcgtagaccc
     1261 gctccccgag gagctccgcg agctggaccg cgcctttctt ctttggcgtg gattcagtca
     1321 acggtcaccc gggggatcct cggcccgacc tggaccagga tctcgtacgg gatggtcccg
     1381 cagacgcggg ccatctcctc cgcgcccatc gacgcctccc ccatcacttc gacgtccgtc
     1441 ccgatcgaca cgcccggcac tccggtaacg tcgatcatcg tgtggtccat gcacaccgtc
     1501 cccgcgacgg ggacggcatg cccggcgacc gacatccggg cacggccggt caacgcgcgg
     1561 cggtagccgt cggcgtaccc tatcgggacg acggcgatcc ggcgggcgcc gtcgcagcgg
     1621 taccgccggt tgtagctcac cgcgtgtccc gcggggagct ccttcaggga catcaccttc
     1681 gtgacgaccc gcatcacggg gcgaagatcc ggcgacgcgt cgccgggagg gaggggggaa
     1741 cagccgtaca tggagatgcc gggccggacg aggtcgaacg gttcttcgcg gaaccggagg
     1801 atccccgcgc tgttcagcgc gtggaccgcg acgccgcccc cgaatgcctt ccgcacggcg
     1861 gggagcatcc cccggaaggc ggccagttgc atcgccgtgt actcgcggtc ctcggccgcc
     1921 ggaccgtccg ccgaggagag gtgcgtcatc cacccttcga gccgcagagc gggggtcgac
     1981 gcgatccgct ccgccgcctc catggcctcc cgcgggagca ggccgagccg ccccatcccg
     2041 gtgtcgacct tgacgtgcac cggaaaggga cgcccttcca cctccgccgc gcgggcgagg
     2101 ttaccgatct gtcccaggtc gaacaggacc gccgaaaggc cgtgagcgtg ggcctccgcc
     2161 gcctgcggcg ggtcgacacc ccccagcacg acgacgggaa ggcggatccc cccctcgcgc
     2221 agctccaccc cttcttccac cgtggccact cccagcatcc gcaacccatc cgcctcgagg
     2281 gcgcgcgcca cggaaagcgc gccgtgcccg taggcgttcg ccttcacgat gccgagaaga
     2341 ccgacgcctt gcgggagcag cgcgcgaatc acttgaacgt tgtgtcggag ggccgagagg
     2401 cggatctccg ccaccgtggg ccgggccaag ccggatcctc ccggggaagc gagataagcg
     2461 ttggagaaag tttaccccac cccctccggg caagtaaagc tcgcgggcac ttgacaccct
     2521 cgcttgccca gccgtatgat tagtggaagc cgccaggggt gcatttcgct gagagcccaa
     2581 gggatacgca ccgtttgcgt cgatccccga gggcaacccg tcgaacctga cccgggtaat
     2641 accggcgcag ggagcgtggt cctcttcgaa ccgtgccgta tccccttggc gcggtttttc
     2701 atcttccgcc cggaaaggat ggtacgccat gacgttgatg tatcgcgcgc ggcaggggga
     2761 gatccctccg gcgatcgccc tcgcggcgca atcggaacat gtggctccgg agaagcttcg
     2821 ggacctggtg gccgtcggga gagtcgtcat cccccgcaac cggaaacgcc gcgagatccg
     2881 ccccgtcgcc atcggcgaag gcctcaccgt caaggtgaac gcaaacgtcg gctcctcccg
     2941 cgaccgcgcc gacatttcgc tggagatgga gaagatgcgc gccgccgtgg cggccggggc
     3001 cgacgcggtg atggacctgt ccacgggagg gccgatcgac gagatccggc gggcgatcct
     3061 cgccgagtgc ccggttccca tcgggaccgt ccccctgtac caggccgcgg ccgacggcaa
     3121 cctgcgggga aaatcgtggg tcgacctcac cgcggtcgac ttcttcgccg cgatcgaaaa
     3181 gcaggccatg gaaggggtcg acttcatgac cctccactgc ggcgtgaccc gcacatcggt
     3241 cgagcggctg gtccgggag
//
LOCUS       DASVJL010000078         2970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1794262, whole genome shotgun sequence.
ACCESSION   DASVJL010000078 DASVJL010000000
VERSION     DASVJL010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794262"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..420
                     /locus_tag="VF357_01425"
     CDS             52..420
                     /locus_tag="VF357_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7522835.1"
                     /translation="MPLSFLGFLAIGAAIGGMSGMFGIGGGAFLVPALVVLCGFDQKM
                     AAGTTLGMLLPPIGIAAFWQYYKADLVNVPAALLLVGGFLAGSYLSAGYAIGLPSLLL
                     KRGFGGLMIATGVIYILTAK"
     gene            447..523
                     /locus_tag="VF357_01430"
     tRNA            447..523
                     /locus_tag="VF357_01430"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:481..483,aa:Pro,seq:cgg)
     gene            complement(628..1401)
                     /locus_tag="VF357_01435"
     CDS             complement(628..1401)
                     /locus_tag="VF357_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEX7522836.1"
                     /translation="MKPFLSACVLAAFLALFLSAPAAAFRKEAGITGDCTECHKLSKE
                     EAGKMLGKIVDNVVAVAPGPFPGVWEVDVVKNGRIFPLYLDYSLKYLFNGQFIRLSDM
                     ANLTGLRYQDLNRVDVASIPVQDAIRIGSKAAKKTVIVLTDPTCPYCVKLHGEIKKAA
                     AKDPDVAFLVMPYPRNPQDMATYRKCQAVVCSRSEKLLDDAYAGKALPEAACKTDAVD
                     KTIRLAERLKIEGTPAMILPDGRMISGYMEAEALLALLR"
     gene            1558..2877
                     /locus_tag="VF357_01440"
     CDS             1558..2877
                     /locus_tag="VF357_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522837.1"
                     /translation="MGRPPTVISFGKWLLPAVVAITLLHPLLPAGTALAADLSLSSKT
                     YLRYYERESAGGNKDTFAPLYEYLSGDAANLGGMPLAFHYYGWGRVDLSDPSGSGRTS
                     GEIGSAYMEYLHPQGNAQAKLGRFFLTEGAAMETIDGAFVKATTPLGLGVSVFGGAPV
                     EYSILNGTRGGSALYGTRVFFVQTGYAEIGASYLRENGTFQEGKDRELFGGDLWLRVA
                     PAVELTAQASYNRSVREMASQRYAVRIVPGATFDISAGYESYTYKGLFQSTLNPVFVF
                     PALDNNDRVRTIFGIVDWAFVPGWTLEVAGKNIRHDKSDPGDANRGEVGVRYTYNDKK
                     DVAGLSATFVSADQTENEYQEYRGFATYSPSKLRLTLDALTQRYKQEIYGKKNQYQVV
                     ATAGYQVLAALHLSGDLTYTQSPNYSKDYAGLLRATLELGTTTGGKK"
BASE COUNT          492 a          947 c          959 g          572 t
ORIGIN      
        1 tcacggtgct ggtctgcggc gagcccctgg ggctatagga gggtccttcc cttgccgctt
       61 tctttcctcg gcttcctcgc gatcggcgcc gcgatcggcg gcatgtccgg catgttcggg
      121 atcggtggcg gcgcctttct cgtcccggcg ttggtcgtcc tttgcggatt cgaccagaag
      181 atggcggccg ggacgacgct ggggatgctt ctccccccga tcggcatcgc cgccttctgg
      241 caatattaca aggcggacct ggtgaacgtc ccggccgcgc tgctgctcgt tgggggcttc
      301 ctggccggct cgtacctgtc cgcggggtac gcgatcggac tgccttcgct cctcctgaag
      361 cggggcttcg ggggactgat gatcgcgacc ggggtgatct atatattgac ggcgaaatag
      421 accgttggta ttatgccgga ttgcgtcggg atgtggctca gcctggtaga gcacctggtt
      481 cgggaccagg gggtcgctgg ttcaaatcca gtcatcccga ccatcatcaa aagaagttgc
      541 gggccgcctt ccccggggcg gccctttttt ctcccctgtc cgattccgtc ctcacggagc
      601 gcgtcccgaa tccgcccccg gtccgcctta ccggagcagc gcgaggagcg cctccgcctc
      661 catgtagccg ctgatcatgc gcccgtccgg gaggatcatc gccggcgtcc cctcgatctt
      721 cagccgctcg gcgagccgga tcgttttgtc caccgcgtcg gtcttgcagg cggcctcggg
      781 aagcgctttc ccggcgtagg cgtcgtcgag cagcttctcg gacctcgagc agaccacggc
      841 ctggcacttc cggtaagtgg ccatgtcttg cgggttccgg gggtagggca tgacgaggaa
      901 cgccacgtcc ggatccttcg ccgccgcttt cttgatctcc ccgtgcagct tcacgcaata
      961 ggggcacgtc gggtcggtca ggacgatcac cgtcttcttc gccgccttgc tcccgatccg
     1021 gatggcgtcc tggaccggga tggaagcgac gtccacgcgg ttgaggtcct ggtaccgcag
     1081 accggtgagg ttcgccatgt cggacaggcg gatgaactgc ccgttgaaga ggtacttcag
     1141 ggaatagtcg aggtagagcg ggaagattct cccgttcttg acgacgtcca cctcccagac
     1201 gccggggaac gggcccggcg cgacggcgac gacattgtcc acgatcttcc cgagcatctt
     1261 ccccgcttct tctttcgata gcttgtggca ctccgtgcag tccccggtga tcccggcctc
     1321 tttccggaac gccgcggcgg gggcggaaag gaacagggcg agaaacgcgg cgaggacgca
     1381 ggcggacagg aacggtttca ttcggggtct ccttgtgcgg ggatcgttac ttcaccccga
     1441 aagaaatgca gatcattttt tgaattatac accggcgcga tgggaaatta ttgttgctac
     1501 ccctccgctg cttaggagag aatgaacatc attccaaatt cttttttggg ggtacgaatg
     1561 ggacgcccgc cgaccgtgat ctctttcggg aaatggctgc tccctgccgt tgtagccatc
     1621 acgctgctcc atcccctcct gccggcgggc acggccctcg cggccgacct ctccctctcc
     1681 tccaagacct acctccggta ttacgagcgg gaatcggcag gcgggaacaa ggacacgttc
     1741 gctccgctgt acgagtacct ctcgggggat gcggcgaacc tgggggggat gcccctcgcc
     1801 tttcactact acgggtgggg gagggtggac ctgtccgacc cgagcggctc cggaaggacc
     1861 tcgggtgaaa tcggcagcgc ctatatggaa tacctgcacc cgcaggggaa cgcccaggcg
     1921 aagctgggcc ggttcttcct cacggaggga gccgcgatgg aaacgatcga cggcgccttc
     1981 gtcaaggcga cgacccccct cgggctgggg gtgtcggtct tcggcggcgc cccggtggag
     2041 tactccattt tgaacggaac caggggggga agcgcccttt acggcacccg ggtcttcttc
     2101 gttcagaccg ggtacgccga gatcggcgcc tcctatctca gggagaacgg gacgttccag
     2161 gaggggaagg accgggagct gttcggcggc gacctgtggc tgcgcgtcgc cccggcggtg
     2221 gaattgaccg cgcaggcgtc gtacaaccgg tcggtccgcg agatggcctc gcagcgctac
     2281 gcggtccgga tcgtgcccgg tgcgaccttc gacatctccg cgggctacga gtcatatacg
     2341 tacaaggggc ttttccagtc cacgctgaac ccggtgttcg tgttccctgc gttggacaac
     2401 aacgacaggg tccggacgat cttcggcatc gtggactggg catttgttcc aggctggacc
     2461 ctggaggtgg cgggaaagaa catccggcac gacaagtcgg atcccgggga cgccaaccgc
     2521 ggggaggtcg gggtgcgcta cacatataac gacaagaagg acgtcgcggg gctctccgcc
     2581 accttcgtat ccgccgacca gacggagaac gagtaccagg aatatcgcgg cttcgccacc
     2641 tattcgccct ccaagctccg gctgaccctg gacgccctca cccagcggta caagcaggag
     2701 atctacggga agaagaacca gtaccaggtg gtcgccacgg cggggtacca ggtcctcgcg
     2761 gcactgcacc tctccggcga cctgacatac acgcaaagcc cgaactacag caaggactac
     2821 gcggggcttc tccgggcgac cctcgagctc gggacgacca cgggaggaaa gaaatgaccc
     2881 gtcgcatgcc tatcccagcc gttctcgggg cggtcctgat cgccctgctc ttcgggtgtt
     2941 ccgcggtctc gcagaccccg tcggtgccgc
//
LOCUS       DASVJL010000079         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1137414, whole genome shotgun sequence.
ACCESSION   DASVJL010000079 DASVJL010000000
VERSION     DASVJL010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137414"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..438)
                     /locus_tag="VF357_01445"
     CDS             complement(40..438)
                     /locus_tag="VF357_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7522838.1"
                     /translation="MEPVGRYKDGQSGTVLLVEDSDVVRDVIAKMLEGGGLSVLQASG
                     GEEALALARRGDAPIDLLLTDIVMPEMSGVELADRLEQEHPAMRILFMTGYAEEAVEN
                     GEILGKHRECIGKPFTQGQITKSVRKILFP"
     gene            651..968
                     /locus_tag="VF357_01450"
     CDS             651..968
                     /locus_tag="VF357_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522839.1"
                     /translation="MPAKSIVSFLVAAFALALFLGPAAAVERTVVGTIVRLDPAGGVL
                     VVRDAAGASWSYKVEADAGIDLSVFRPGDRVKVSIGRATPLNMITAADLLRKGDRVEK
                     APY"
     gene            1005..1133
                     /locus_tag="VF357_01455"
     CDS             1005..1133
                     /locus_tag="VF357_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522840.1"
                     /translation="MSKWVILCPACGEEFKVDEEVVPARCPLCEYEGDFEVVGADD"
     gene            1152..>1705
                     /locus_tag="VF357_01460"
     CDS             1152..>1705
                     /locus_tag="VF357_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522841.1"
                     /translation="MAGPDLLLIHPPAARAAEPPLGTAVLLSHLRQGGATAEAIDANL
                     EAHLYLLDGDRLAAAAGPAPTTALHRAMKNVPRALSFLRSPAAGRSFPRYTTAARHLN
                     TALSAYRGESGRERWTLGDYVHGNLSEFSTLDLSLAASGKAGTVFSGYFRDALLPRVD
                     RMRPRGVAISVNYRHQVLPAFELAG"
BASE COUNT          252 a          584 c          562 g          307 t
ORIGIN      
        1 gcctgacgca gcagcagccg gcggtggcgc gggggctagt taggggaaaa ggattttccg
       61 cacactcttc gtgatctgcc cttgggtgaa cggtttcccg atacactccc tgtgcttccc
      121 gaggatctcc ccgttctcga cggcttcctc ggcgtacccg gtcatgaaaa ggatgcgcat
      181 ggcggggtgt tcctgctcca gccggtcggc cagctccact ccgctcatct ccggcatgac
      241 gatgtcggtc agcaggaggt cgatcggcgc gtcccctcgg cgtgccagcg ccaacgcctc
      301 ctccccgccg gaggcctgga gaacggagag accgccgccc tccagcatct tggcgatcac
      361 gtcgcggacc acgtccgaat cctcaaccag caggaccgtc ccgctctgcc cgtccttgta
      421 ccgtcccact ggctccatcg tgcccgggcc gtcctttccg ggggtgtccc ggcaaaccct
      481 gtcctttggg taatacgata tcttatcgtg gaagcgcacg attcggacca ttgtgcttcg
      541 gtgctccctc cgcaccgtat tgtcacaact cagcccgggg gctgtcctcg catctcccgc
      601 gatacgcggt aagatgaaaa tagtacgacg tccgcttgag gggatggaga atgcccgcga
      661 aaagcatcgt gtccttcctc gtcgcggcct tcgctctggc gctgttcctc ggaccggctg
      721 ccgcggtgga acggacggtc gtggggacga tcgtccggct ggatcccgcg gggggcgtgc
      781 tggtcgtgcg ggatgccgcg ggagcgtcct ggagctacaa ggtcgaggcc gacgccggga
      841 tcgacttgtc ggtattccgc ccgggggacc gggtcaaggt gtcgatcggg cgcgcaactc
      901 cgctcaacat gatcacggcg gcggacctgc tgcgcaaggg ggaccgggtc gagaaggccc
      961 cgtactgacc ggtcgggcgg cgcaacccga aaggaggcgg tttcgtgagc aaatgggtca
     1021 tcctttgccc tgcgtgcggg gaggagttca aggtcgacga ggaagtggtc ccggcgcgct
     1081 gccctctttg cgagtacgag ggggacttcg aggtcgtggg cgcggacgac tgatgtccgc
     1141 cgccgggtga catggccggt ccggacctcc tgctgattca cccgcccgcc gcccgggccg
     1201 cggaaccgcc gctggggacg gccgtgctcc tgtcccacct gcgccaggga ggtgcgacgg
     1261 cggaagcgat cgacgccaac ctcgaggcgc acctttacct gctggacggg gatcggctgg
     1321 cagccgccgc cggccccgcg ccaacgaccg ctctccaccg ggccatgaag aacgtgcccc
     1381 gggcgctctc cttcctccgg tcccccgcgg ccggaagatc gttcccccgc tacaccaccg
     1441 ccgcgcgcca cctgaatacg gcgttgtccg catatcgcgg cgagagcggg cgtgaacgat
     1501 ggacgctcgg cgactacgtg cacggtaatc tctccgagtt ttcgacgctg gacctatctc
     1561 tcgcggcgtc cgggaaagcg ggaacggtct tctccggcta cttccgggac gctctcctcc
     1621 ctcgcgtcga ccggatgcgt ccgcgcggtg tcgcgatctc cgtgaactac cggcaccagg
     1681 tcctccccgc ctttgagctt gcggg
//
LOCUS       DASVJL010000080         4936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_11354, whole genome shotgun sequence.
ACCESSION   DASVJL010000080 DASVJL010000000
VERSION     DASVJL010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11354"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VF357_01465"
     CDS             complement(<1..208)
                     /locus_tag="VF357_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522842.1"
                     /translation="MTTRFLLGLARRIDARPGKIAVAVLLVAAASGGIVARIPVRTNL
                     LEVLPEGNPTIRAFRGFLEDFGMMD"
     gene            complement(209..1000)
                     /locus_tag="VF357_01470"
     CDS             complement(209..1000)
                     /locus_tag="VF357_01470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7522843.1"
                     /translation="MVRKGYPIVRCGGCGLVYVHPRPPKEELPGLYGDYHTRDGGDEA
                     SWNRLMEKVFRDAADRLDLLRNGSGPPRLLDVGCGYGGFVSLMRDRGWDAEGVDPSPM
                     TVAAASAKGIPVRLGTLDEFSRPGAAYNAITMFYVLEHLFDPMSALRKVCALLEPGGV
                     LLVRVPDTTPIVRLLSPFGLGDGLYDPPFHLFDFPPRVLTMMLAEAGFGKIRTFPGHN
                     TTPPRIAPRLATLLFGALARGLFAGSGGRILLPGASKTTIARKPS"
     gene            complement(1086..1373)
                     /locus_tag="VF357_01475"
     CDS             complement(1086..1373)
                     /locus_tag="VF357_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX7522844.1"
                     /translation="MDETKKKIREILSVRLNLSTAVEEIEDDAPLFGPGSLGLDSIDA
                     LELVLGIQKEFGVGIEDRFLAVKVLVSIDTIAEYVKSQSAGNPESAGGLNA"
     gene            complement(1599..3050)
                     /locus_tag="VF357_01480"
     CDS             complement(1599..3050)
                     /locus_tag="VF357_01480"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7522845.1"
                     /translation="MNVLLLRPNPGNERFGLGPFFRVEPLGLEYIGAALRAHGHAPTV
                     ADLRFRPGASSWVRRTRPRVVGISCTHALEYDRVRETALEVRRASPEVFVVVGGHAAA
                     CHPSPLEVDEIDAVCLDDGEEIVPALVNAVGRGTPLETVPMLRLRTRDGWVSTPPSPG
                     RTSLDGVPLPARDLVSRHRNGYHCLLFKPVGLVETARGCPHRCSFCSVWQLYGRSFRE
                     RSIGAVVEDFATAGDSIFVADDLFWNHAERSLELAGALRKRGVKKRWILVQTRTDMIC
                     RQQGLLEAWRPLARDFDIFLGLEAATDAGLSRVDKDVPVSESVEAVRIARSLRYGVTG
                     NFLVDPGWGEEDFRRLWEFVTCHGLQRAGYTILTPLPGTDYFHELAPRLAGQPWSKFD
                     MHHVLWEPRLGARRFFELYAETWRKSILNTSGEKRWVDWMRQIRPAEIPYLARVLWRT
                     QRMMKAGAYLREYGKTDRAPMPVRFPSVTGASR"
     gene            3166..3894
                     /gene="fabG"
                     /locus_tag="VF357_01485"
     CDS             3166..3894
                     /gene="fabG"
                     /locus_tag="VF357_01485"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX7522846.1"
                     /translation="MAQNDIALITGGSKGIGAAIARALARDGFDIWLNYRSDHEAAKE
                     VARQVGELGRTCKLLPFDVADSGSVKCALAPVLETDRPLVLVNNAGFSRDGLLVWMEE
                     PEWKETLAVHLDGFFFVTKMVLFGMLKRRSGRIINIVSTSGQTGVAGQTNYSAAKAGL
                     IGATRSLALEVAKRNILVNAVSPGFIETDMTAGLPLDRILPLIPLGRMGKPEEVAEVV
                     AFLASGKASYITGQVLSVNGGVYL"
     gene            3911..4351
                     /locus_tag="VF357_01490"
     CDS             3911..4351
                     /locus_tag="VF357_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522847.1"
                     /translation="MKPEYALPIAASLLIPHRTPMRLVDTLLSVHEDRGVTESVFPRT
                     SMMADEKGRLDEVAFLEMIAQSYAALKGYTDLVKGKPAGEGFLVGVRHLEVTGRAYAG
                     DRLLTSIRTVTSFGGFAVAEGSVTRGDETIASGIIKLWLVGEGR"
     gene            4348..4734
                     /locus_tag="VF357_01495"
     CDS             4348..4734
                     /locus_tag="VF357_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522848.1"
                     /translation="MNALRRGIRASAAGEIARTGPETVMRRYRFAPGFIGFSGHFPGN
                     PILPAIVQVCAVVSLAEEEGGKALRLEAVRAAKFLSPIRPDEEVSIRYRRRVESGEDL
                     CDATISVAGKTSAAFQLRFAEEESLR"
     gene            4731..>4936
                     /locus_tag="VF357_01500"
     CDS             4731..>4936
                     /locus_tag="VF357_01500"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX7522849.1"
                     /translation="MITPYFTAEAGAPAPLRAALSGRVRFEEVDPLGIVWHGRYASYF
                     EDARVALTDRYGIGYLDLYEKGVVA"
BASE COUNT          931 a         1590 c         1568 g          847 t
ORIGIN      
        1 tatccatcat gccgaaatcc tcgaggaacc cccggaatgc gcggatcgtg ggattccctt
       61 ccggcagaac ctccagcagg tttgtccgga ccgggatgcg cgccacgatc ccgccggacg
      121 cggcggcgac gagcaggacg gcgacagcga tctttcccgg tcgcgcgtcg atcctgcgag
      181 ccaggcccag aagaaaccgg gtcgtcattc aggaaggctt tcgcgcgatg gtggttttac
      241 tcgccccggg gaggaggatc ctgccgccgc ttcccgcaaa caggcctcgg gcaagcgcgc
      301 cgaacaggag agtggccaac cggggcgcga tgcggggggg ggtcgtgttg tggcccggga
      361 atgtccgtat cttcccgaat cccgcctcgg cgagcatcat cgtgagcacg cggggcggaa
      421 aatcgaagag atgaaagggg ggatcgtaca acccgtctcc caacccgaaa ggggaaagca
      481 gacggacgat cggcgttgtg tcgggaaccc gcaccaggag caccccgccc ggttcgagga
      541 gtgcgcatac ctttctcaac gcggacatcg ggtcgaagag gtgttccaac acatagaaca
      601 tggtgatcgc gttgtatgcc gcgcccggac gggaaaattc atcgagggtt cccaatcgga
      661 cgggaatgcc tttcgccgat gcggcggcaa ccgtcatcgg ggacggatcg accccctccg
      721 cgtcccaccc ccgatcccgc atcagcgaga cgaatccacc gtacccgcac cccacgtcca
      781 gaaggcgggg cggaccggaa ccgttcctca aaaggtcgag gcggtcggcc gcatcccgaa
      841 agaccttctc catgaggcgg ttccaactcg cctcgtcccc gccgtcacgg gtatggtaat
      901 ccccgtagag cccgggaagt tcctccttcg gtggcctcgg atggacgtag accagcccgc
      961 atccaccgca acggacgatg gggtatcctt tccggaccac gatgggttgc ggcgcatcct
     1021 ggccgcagag gatgcatgga gtaaaacatg tcttcgacac tttattgaac ccgcatcacc
     1081 gggagctacg cgttgagtcc gcccgccgat tcggggttcc ctgccgattg ggacttcaca
     1141 tattccgcga tggtatcgat ggataccagc accttcaccg ccaggaagcg atcctcgatg
     1201 ccgacgccga actccttctg gatgccgagc accagttcca gcgcgtcgat cgagtcgagt
     1261 ccgaggctgc cgggcccgaa cagtggcgca tcatcttcga tctcctcgac cgccgtggag
     1321 agattcagac gaacggagag gatttcacgg atcttctttt tcgtttcatc cacttccagc
     1381 gcgtttcctc cttctttccc gtcgatgaac ccggggggaa tacatctgcc ggcaattttt
     1441 ttatcataag gcgcgaaacc tctccgggca acggaaaagt ctttccggat gatacccgtc
     1501 gtgccgcaaa cgcgcaatcc gtgcggtctt gcggcagcgg atccggatcg aggagacggc
     1561 gtgggcggaa atcgtcgcgt cggtgggcgg ggctcgggtt agcgcgatgc accggtgacg
     1621 gaagggaacc ggacgggcat cggggcacga tccgtcttcc cgtactcgcg gaggtaggcc
     1681 ccggctttca tcatcctctg ggtgcgccat agaacgcgcg caaggtacgg tatctcggcc
     1741 ggccggatct gccgcatcca gtcgacccac cgtttctcgc ccgacgtgtt gaggatcgac
     1801 ttgcgccagg tctccgcgta gagttcgaaa aaccgtctcg caccgaggcg aggctcccag
     1861 aggacgtggt gcatgtcgaa tttggaccag ggctgccccg cgagtcgagg cgcgagttcg
     1921 tggaaataat ccgttcccgg caacggagtg aggatggtgt accccgcccg ctgcaacccg
     1981 tggcaggtga cgaactccca gagccggcgg aaatcctcct ctccccagcc gggatcgacc
     2041 aggaagttgc cggtcacgcc gtatcgcagg gaccgcgcga tccgtaccgc ctcgacgctc
     2101 tccgaaaccg ggacatcctt gtccacccgg gacaggcccg cgtcggtcgc ggcctccagt
     2161 ccgaggaaaa tatcgaagtc ccgcgccagg ggcctccacg cctccagcag cccttgctgt
     2221 cggcagatca tgtcggtgcg tgtctgcacg aggatccagc gttttttcac tccgcgcttc
     2281 cgaagggccc ccgcgagttc gaggctccgc tccgcgtggt tccagaacag atcgtcggcg
     2341 acaaagatcg aatcccccgc cgtggcgaaa tcttcgacga cggccccgat ggaccgttcc
     2401 cggaaggatc gcccgtacaa ttgccacacg gaacagaaac tgcaacggtg cgggcatcca
     2461 cgggccgtct ccacgagtcc caccggcttg aagaggaggc aatggtagcc gttgcggtgg
     2521 cggctcacca agtcgcgcgc cggcaagggg acgccgtcaa gggatgtccg cccgggagag
     2581 ggcggcgtcg acacccaacc gtcccgtgtc cgaaggcgga gcatgggcac cgtttccagg
     2641 ggggtcccac gcccgacggc attgaccagc gccggaacga tctcctcccc gtcgtcgagg
     2701 cagaccgcgt cgatctcatc gacctcgagc ggggaggggt ggcacgccgc cgcatgcccg
     2761 ccgacgacca cgaaaacctc aggcgacgcc ctccggacct cgagcgcggt ctctcgaacc
     2821 ctgtcgtact ccaaggcgtg cgtgcaggag atgccgacga cccgcggccg cgtccgccgc
     2881 acccaggagg atgcgccggg gcgaaagcgg agatccgcca ccgtcggagc gtggccgtgg
     2941 gcgcgcaacg ccgccccgat gtactcgagg ccaagcggtt caacgcgaaa gaaagggccg
     3001 agaccgaacc gctcgtttcc cgggttcggc ctcaggagca gtacattcat gccgcaccat
     3061 cctgatttcg taacctccaa gggtacacgg ctttcccgat tttgcaccgg aaaaaccttt
     3121 cccccttgac gggagcatgc caattggagg ttaaataatt gccttatggc ccaaaatgac
     3181 atcgccttga tcaccggcgg cagcaagggg atcggggccg ccatcgcccg cgccctggcg
     3241 cgggatggtt tcgacatctg gctcaactat cggtcggacc acgaagcggc gaaggaagtc
     3301 gcccggcagg tcggcgagtt ggggaggacc tgcaaactcc tccccttcga tgtcgccgat
     3361 tccggctcgg tcaaatgcgc cctcgccccg gtgctcgaga cggatcgtcc cctggtcctc
     3421 gtcaacaacg ccggcttcag cagggacggc ctgttggtct ggatggaaga accggaatgg
     3481 aaggaaaccc ttgccgtcca cctggacggg tttttcttcg tcacgaagat ggttttgttc
     3541 ggaatgctga agagaagatc cggacggatc atcaacatcg tctccacatc aggacaaacc
     3601 ggcgtggcgg gtcagacgaa ctactcggcg gccaaagcgg ggctgatcgg ggcaaccagg
     3661 tcgctggccc tggaagtcgc caaaaggaac atcctggtca acgccgtttc ccccggattc
     3721 atcgaaacgg acatgaccgc cggtcttccc ctcgaccgga tcctgccgtt gatcccgttg
     3781 gggcggatgg ggaaaccgga agaagtggcg gaggtggtcg cctttctcgc atcgggaaag
     3841 gcctcctata tcaccgggca ggttttatcg gtaaacggcg gcgtctatct gtaacccccc
     3901 cggccgagac atgaaacccg aatatgcgct tcccattgcg gcttcgctgc tcatcccgca
     3961 ccggacgccg atgcgcctcg tggatacgct gctctccgtg cacgaggacc gcggagtcac
     4021 cgagtccgtc tttccgcgaa ccagcatgat ggcggacgag aagggaaggc tcgacgaggt
     4081 ggcgttcctg gagatgatcg cgcagagtta cgccgcgttg aagggataca cggacctggt
     4141 gaaaggaaaa cccgcggggg aaggcttcct ggtcggagtg cgccacctgg aggtcaccgg
     4201 cagggcgtat gccggcgacc gcctcctgac ctcgatccgg accgtcacgt cgttcggcgg
     4261 cttcgccgtc gccgagggca gcgtcacccg aggggacgaa accattgcgt ccgggatcat
     4321 caagctatgg ctcgtggggg aaggacgatg aatgcgctgc gaagggggat ccgcgcctcg
     4381 gccgcggggg agatcgctcg gaccgggccg gaaacggtca tgcgccgtta ccgcttcgcc
     4441 cccggtttca tcggcttctc aggccatttt ccgggcaatc ccatcctccc cgcgatcgtc
     4501 caggtgtgcg ccgtggtctc cctcgcggag gaagagggcg ggaaggccct gcgccttgag
     4561 gccgtgcggg ccgcgaagtt tctttcaccg atccgccccg acgaggaggt atcgatccgg
     4621 taccgtcggc gcgtcgaatc cggggaagac ctctgcgacg ccacgatctc ggtcgcggga
     4681 aaaacgtccg ccgccttcca actccgattt gccgaggagg agagtctccg atgatcacgc
     4741 cgtattttac cgccgaggca ggagcgcccg ccccgctgcg cgctgcgttg tccggacgcg
     4801 tgcgattcga ggaggtcgac ccgctgggga tcgtctggca cggacggtac gcaagctatt
     4861 tcgaggatgc ccgcgtggcc ctcaccgacc ggtacggcat cggctacctc gatctttacg
     4921 aaaaaggtgt cgtcgc
//
LOCUS       DASVJL010000081         3701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1512760, whole genome shotgun sequence.
ACCESSION   DASVJL010000081 DASVJL010000000
VERSION     DASVJL010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512760"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VF357_01505"
     CDS             <1..815
                     /locus_tag="VF357_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7522850.1"
                     /translation="VLCPGSYRLGNRVFRCWREKGHGAIDMYRAIVQSCDVYFYTLGL
                     KLGPDRLAKLEKDAGLGTITRIDLPGEQKGLVPDTVWKRTVVKERWYDYESVILGIGQ
                     GAIHLTPLEMAVGYATLATGGEVMRPRVVTKVIGKDGKVRTFAQEMLRKLPWDPENVE
                     FIRKALAGVVNDYGTGGSVKLPGIVVGGKTGTAQVASVKGKMIKSEDLPYEIRDHAWF
                     VAFAPVDDPQIVVAAMVEHGGHGASAAGPIVKAVMQEFFRTRPVGPPRKEGA"
     gene            812..1945
                     /gene="rodA"
                     /locus_tag="VF357_01510"
     CDS             812..1945
                     /gene="rodA"
                     /locus_tag="VF357_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX7522851.1"
                     /translation="MSRPSKWARMEWPLLVEALALCAIGLLNVYSGTRVHGVPGTALV
                     TKQLVWILLGVGAFFAVFLMSDGFIEETANGFFLAVLLLLLVVLLAGRVRGGAQRWIA
                     IGAFNFQPSEFAKLAVTVALARYFSGTYRYGGIGLQEVLPAIGLVLAPFLLVVLQPDL
                     GSAGVFLFILAGMAVVACVRWRVLAFFAASAAAAVPALWFFMKEYQRQRVLTFMNPEL
                     DPLGAGYHVIQSKIAVGSGGIIGKGYLQGTQGSLRFLPEQHTDFAFAVFSEEWGFLGS
                     LLLLVLFLLLVYRLFFLTARSQDRFASFACGGIAVFFLTHIVINLAMVCGLFPVVGIP
                     LPFVSYGGSSMLTGMMALGAAANLSRSRFTFQGGGGKGRGIAS"
     gene            complement(1899..2489)
                     /locus_tag="VF357_01515"
     CDS             complement(1899..2489)
                     /locus_tag="VF357_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522852.1"
                     /translation="MRKGASFAALIAGLFLMAGCGPKAIIVGSRPFPAGHPVFSDASG
                     KALETLPAAEGTIRLVFLEFPWCTACADVWRAMRTAAAPFPPGTVRIYRVLFDRETLL
                     SRAEQREVPPLHPAPLPEGDAPEGPGALEITTLTALPGEFRKEFRVSHGPLLLLLDAE
                     GKVERRWIGFSAGMSLELSSEIRKRSRDLSPRPPGT"
     gene            complement(2486..2809)
                     /gene="trxA"
                     /locus_tag="VF357_01520"
     CDS             complement(2486..2809)
                     /gene="trxA"
                     /locus_tag="VF357_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7522853.1"
                     /translation="MAGNILELNSANFQSTVEGGTPVLVDFWAPWCGPCRVIAPILEE
                     VAKEFDGKTRVGKVNVDDSPEIASRFGVRGIPTLILFKDGQIKGQMVGVNPKSNIVSL
                     VQKNL"
     gene            complement(2833..3231)
                     /locus_tag="VF357_01525"
     CDS             complement(2833..3231)
                     /locus_tag="VF357_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX7522854.1"
                     /translation="MEQRIETILSESKTVAVVGISDKPDRPSHGVAKYLQERGYRVIP
                     VNPLLTEVLGEKAYKSLAEIPGRVDLVDVFRKSADVPPIAEEAVRIGARFFWMQEGVE
                     SDRAREILDAAGIPCVMDRCVKKELAKRGK"
     gene            3372..>3701
                     /locus_tag="VF357_01530"
     CDS             3372..>3701
                     /locus_tag="VF357_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7522855.1"
                     /translation="MKESAVRSLKEGSDLRLKMAETMPAEIATAATAIAEAFKAGRKL
                     LLFGNGGSAADAQHIAAEFMNRFLIERPPLPAIALTTDTSVLTSIANDYAFDEIFSKQ
                     VKALGKKG"
BASE COUNT          584 a         1180 c         1247 g          690 t
ORIGIN      
        1 cggtcctctg ccccgggtcg taccgtctcg ggaaccgggt cttccgctgt tggagggaga
       61 aggggcacgg cgccatcgat atgtaccggg cgatcgttca gtcgtgcgac gtctactttt
      121 acacgctcgg gctgaagctg ggccccgacc gtctggcgaa gctggagaag gacgcggggc
      181 tggggacgat cacaaggatc gacctccccg gggagcaaaa ggggctggtc ccggacacgg
      241 tgtggaagcg caccgtggtc aaggagcgat ggtacgatta cgagagcgtg atcctcggga
      301 tcggccaggg ggcgattcac ctcacgcccc tcgagatggc cgtcggctac gcgacgctgg
      361 cgacgggcgg cgaggtgatg cggccccgcg tcgtgacgaa ggtgatcggc aaggacggaa
      421 aggtgcgtac gttcgcccag gagatgctga ggaaactccc atgggacccg gagaacgtgg
      481 aattcatccg aaaggcgttg gccggcgtgg tgaacgacta cggaacgggc gggagtgtga
      541 agcttcccgg gatcgtggtg ggcgggaaga cgggcaccgc ccaggtcgca tcggtgaagg
      601 ggaagatgat caagtccgaa gatcttccgt acgagatccg cgaccacgcg tggttcgtcg
      661 cattcgctcc ggtggacgat ccgcagatcg tcgtggcggc gatggtggag cacggcgggc
      721 acggcgcgtc cgccgccggg ccgatcgtca aggcggtgat gcaggaattc ttccgcaccc
      781 gacccgtcgg cccgccgcga aaggagggcg cgtgagccgt ccgtcgaaat gggcacggat
      841 ggaatggccc ctgctggtcg aagcgctggc cctctgcgcg atcgggctcc tgaacgtcta
      901 ctccggcacg cgggtccacg gcgttcccgg gaccgcgctg gtgaccaagc agctcgtctg
      961 gatccttctc ggggtcggcg cctttttcgc cgtcttcctc atgagcgacg gcttcatcga
     1021 agagaccgcg aacggtttct tcctcgccgt gctccttctc ctattggtgg tgctgctggc
     1081 agggagggtc cgcggtggcg cccagagatg gatcgccatc ggcgcgttca acttccagcc
     1141 gtcggaattc gcgaagctcg ccgtcacggt cgcgctggca cggtacttct cgggaacgta
     1201 ccgatacggg gggatcgggc tgcaggaggt ccttccggcg atcggcctgg tcctcgctcc
     1261 cttcctcctg gtggtgctcc agcccgatct tggaagcgcc ggggtgttcc tcttcatcct
     1321 cgcgggaatg gcggtggtgg cgtgcgtgcg gtggagggtg ctcgcgtttt tcgcggcgtc
     1381 cgccgccgcc gcggttcccg ccctctggtt cttcatgaag gagtaccaga ggcagcgggt
     1441 gctcaccttc atgaaccccg agctcgaccc gctgggggcg gggtatcacg tgatccagtc
     1501 gaagatcgcg gtggggtcgg gcgggatcat cgggaagggg tacctgcagg ggacccaggg
     1561 gtcgctccgg ttcctgccgg agcagcacac cgatttcgcc ttcgccgtct tctccgagga
     1621 gtgggggttc ctcgggtccc tcctgctgct cgtcctgttc ctgctgctgg tctaccgcct
     1681 cttcttcctt accgcccggt cgcaggatcg gttcgcgtcg ttcgcctgcg gggggatcgc
     1741 ggttttcttc ctgacccaca tcgtcatcaa cctggcgatg gtgtgcggcc tcttccccgt
     1801 cgtggggatc ccgctcccgt tcgtgagcta cggcgggtcg tcgatgctga ccgggatgat
     1861 ggcgcttggt gcggcggcga acctgtcccg ttcccggttc acgttccagg ggggcggggg
     1921 gaaaggtcgc gggatcgctt cctgatctcc gaggagagtt ccagggacat ccccgccgag
     1981 aaaccgatcc accgcctctc cacctttcct tcggcgtcga gaaggagcaa caggggcccg
     2041 tggctcacgc ggaactcctt gcggaactcg ccggggagcg cggtgagggt ggtgatctcc
     2101 agcgccccgg gaccctccgg ggcgtcgcct tcggggagag gggcagggtg caatggaggg
     2161 acctcccgct gttcggcgcg ggacagcagc gtttcgcggt cgaacaggac ccggtagatt
     2221 cgcacggtgc cgggaggaaa cggcgccgcg gcggtccgca tcgccctcca gacgtccgcg
     2281 cacgcggtac accacgggaa ctcgagaaag acgagccgga tcgtcccttc ggctgccggg
     2341 agcgtctcaa gggccttccc ggaggcgtcg gagaaaacgg gatgcccggc cgggaagggg
     2401 cgggatccca cgatgatcgc cttgggtccg catcccgcca tcaggaacag acccgcgatc
     2461 agcgccgcga aggaggcgcc ctttctcaaa ggttcttctg caccagcgaa acgatgttgg
     2521 atttcgggtt gacgccgacc atctgtccct tgatctggcc gtccttgaag aggatgagcg
     2581 tcgggatccc ccgcacgccg aaccgcgatg cgatctccgg gctgtcgtcg acgttcacct
     2641 tccccacgcg tgtcttcccg tcgaattcct tcgcgacctc ctcgaggatc ggggcgatga
     2701 cgcggcacgg tccgcaccac ggggcccaga agtcgacgag gacgggggtt ccgccctcca
     2761 ccgtcgactg gaagttcgcg ctgttcagtt cgaggatgtt tccggccatt ccgtccctcc
     2821 ctgcgtcttg ggttatttcc cccgctttgc cagttccttt ttcacgcaac gatccatcac
     2881 gcacgggatc ccggccgcgt ccaggatctc gcgggcgcga tcgctttcca ccccttcctg
     2941 catccagaag aagcgggcgc cgatccgcac ggcttcctcc gcgatcggag gcacgtccgc
     3001 cgatttgcgg aacacgtcga cgaggtcgac ccgcccgggg atctccgcga gcgacttgta
     3061 cgccttctcc ccgagaacct cggtcagaag gggattgacc gggatcacgc ggtatccccg
     3121 ctcctgcagg tacttcgcca cgccatggct gggccgatcc ggcttgtcgg agatcccgac
     3181 gacggcgacc gtcttcgact cggagaggat cgtctcgatc cgctgttcca tcattcctcc
     3241 atcacacttt cagatgcggt tcatgctaca tcggtttttt ccgaatgaaa atagattccc
     3301 ccgcggcgtt ccttgacagc ccgcgagaga aaagattatc gtttccctat tcttgtcgga
     3361 gggagctctc cttgaaggaa tcggccgtgc gttcgttgaa ggaagggtcc gacctgcgcc
     3421 tgaagatggc ggaaacgatg ccggcggaga tcgcgaccgc cgcgacggcg atcgccgagg
     3481 cgttcaaggc gggccggaag ctcctcctct tcggcaacgg cggcagcgcc gcggacgcgc
     3541 agcacattgc cgcggagttc atgaaccggt tcctcatcga gcggccgccc ctgcccgcga
     3601 tcgccctcac cacggacacc tcggttctca ccagcatcgc caacgactac gcgttcgacg
     3661 agatcttcag caagcaggtg aaggccctcg ggaagaaggg g
//
LOCUS       DASVJL010000082         3399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1324681, whole genome shotgun sequence.
ACCESSION   DASVJL010000082 DASVJL010000000
VERSION     DASVJL010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324681"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /gene="prmA"
                     /locus_tag="VF357_01535"
     CDS             <1..900
                     /gene="prmA"
                     /locus_tag="VF357_01535"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX7522856.1"
                     /translation="SVQTRREAVDAVTQFFVAHGSLGTAHDEQLLGAEGDPADPIPPP
                     PKVTRLTAYFPFDTDLHALKQAFLDLLPVLSESFGPGPEAFSDATEITDAGWAEKWKE
                     HFHARKIGRKIVIKPSWETVEEGEREVVLTVDPGQAFGTGTHETTQMCLRMIEDVFDL
                     SPPPRDVLDVGTGTGILGIAAARLGAKRVLAVDTDPVAVDVAAKNAVENGVADVFRAE
                     TTPLSAIPGVFDLILGNLIAEILMDMAPELLRRTTPGGHLVLSGILEEKSGWVVEEFV
                     KNGASLLAESADGQWTALLFRRE"
     gene            914..1657
                     /locus_tag="VF357_01540"
     CDS             914..1657
                     /locus_tag="VF357_01540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX7522857.1"
                     /translation="MPTFFVDRKNISGDTAILSGTEAGHMLRSLRLSVGDSFFAFDED
                     GNRYRMRILEATSRSLRAEILEAFPPEPPPDVAVSLFVGLPKADKMDFILEKATELGV
                     SRVIPFRSSRTIPRLDPTDARKRLSRWERVALAAAKQCGSGRVPEVSGIVPYPEALRR
                     AAEHEGKIVFYEGEGTFSLKRVLSGMGGSRTVALIVGPEGGFSPDEVREAVAAGCRRA
                     GLGSRILRVETAALAVLSMVMYHFEKESA"
     gene            1661..2233
                     /locus_tag="VF357_01545"
     CDS             1661..2233
                     /locus_tag="VF357_01545"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7522858.1"
                     /translation="MASSTVHPHFAGFWSRAAARIIDLLIIIAAFNLIYVVDRIGADA
                     GLWTGMRLDEGSLTGAGFSMANILRGLFFLTFPVFYYVYLHATYGQTFGKMALKIKVV
                     NEDGTPLDYRKAFLRWLGYFLCDLTFYIGYLWAAFDPRKQGLHDKVCKTVVVRTDVPF
                     PLPEAVEPEAPAETPPGEPASLDTEASPPI"
     gene            2252..2327
                     /locus_tag="VF357_01550"
     tRNA            2252..2327
                     /locus_tag="VF357_01550"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2285..2287,aa:Phe,seq:gaa)
     gene            3151..>3399
                     /locus_tag="VF357_01555"
     CDS             3151..>3399
                     /locus_tag="VF357_01555"
                     /inference="COORDINATES: protein motif:HMM:NF015751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HEX7522859.1"
                     /translation="MWQIPIKRTLDRIPGGMMIVPLLFGAVINTFFPTTPKFFGSFTG
                     ALFTGALPILAVFYVCMGASIDVKATPYILKKGGSLFAV"
BASE COUNT          626 a         1060 c         1030 g          683 t
ORIGIN      
        1 tccgtccaga cccgccgcga ggcggtcgac gccgtcaccc agttcttcgt cgcccacggt
       61 tccctcggga cggcccatga cgagcaactg ctcggcgcgg aaggggaccc ggcggacccg
      121 atcccccctc cgccgaaggt gacccgcctg accgcgtatt tcccctttga caccgacctc
      181 cacgccctca aacaggcgtt cctcgatctc ctcccggtcc tttcggagtc gttcgggccc
      241 ggcccggagg cgttcagcga cgccaccgag atcaccgacg cgggttgggc ggaaaagtgg
      301 aaggagcatt tccacgcacg gaagatcggc cggaagatcg tgatcaagcc ttcctgggag
      361 acggtcgagg agggtgagcg cgaggtcgtg ctgacggtcg accccggcca ggccttcgga
      421 accggcaccc acgagaccac gcaaatgtgc ctgcggatga tcgaggatgt gttcgacctc
      481 tccccgcctc cccgtgacgt cctcgacgtg gggacgggaa cggggatcct cgggatcgcc
      541 gcggcgcgcc tcggcgcgaa gcgcgtcctt gcggtcgaca ccgacccggt ggcggtggac
      601 gtcgccgcga agaacgccgt ggagaacggt gtggcggacg tgttccgcgc ggagaccact
      661 cccctatccg cgatccccgg cgtcttcgac ctgatcctgg gaaacctgat cgccgaaatc
      721 ctgatggaca tggccccgga actcctccgg cggacgaccc cgggcggaca cctcgttctc
      781 tccggcatcc ttgaggagaa gagcggctgg gtggtcgagg agttcgtgaa aaacggcgcc
      841 tcccttctcg ccgaatccgc cgacgggcaa tggacggccc tcctcttccg ccgggagtga
      901 tcggggaaac gcgatgccga ccttcttcgt cgaccgaaag aacatctccg gcgacaccgc
      961 catcctctcc gggaccgagg cgggacacat gctccgctcc ctgcgcctct ccgtgggcga
     1021 ctccttcttc gccttcgacg aggatgggaa ccggtaccgg atgcggatcc tcgaggcgac
     1081 gtcgcgctcc ctgcgggccg agatcctcga ggcgttcccg ccggaacctc cgcccgacgt
     1141 cgccgtctcc ctgttcgtgg gactcccgaa agccgacaag atggacttca tcctcgaaaa
     1201 agcgacggag ctgggcgtgt cacgggtgat cccgttccga tcgtcccgca cgatcccccg
     1261 cctcgatccg accgacgccc ggaaacggtt gtcgcgatgg gagcgggtgg cgctggccgc
     1321 cgcgaagcag tgcggttccg gccgcgtccc ggaggtgtcc gggatcgtcc cgtaccccga
     1381 ggcgctgcgc cgcgcggcgg agcacgaagg gaaaatcgtc ttctacgaag gcgaagggac
     1441 gttctcgctg aagagggtcc tctccggaat gggagggtcc cgcaccgtcg ccctgatcgt
     1501 cggccccgag ggcggcttct ccccggacga agtccgggag gcggtcgcgg cggggtgccg
     1561 gcgcgcgggt ctggggagcc ggatccttcg cgtcgagacg gcggcgctcg cggttctctc
     1621 catggtgatg taccatttcg aaaaggagtc cgcgtaaccc atggcgtcgt cgacggtaca
     1681 tccccacttt gccgggttct ggtcgcgcgc cgcggcccgg atcatcgacc tgctcatcat
     1741 catcgcggcg ttcaacctga tctacgtggt ggaccggatc ggtgcggacg cggggctgtg
     1801 gacgggcatg aggctggacg aggggagcct gaccggcgcg gggttctcca tggccaacat
     1861 ccttcgcggc ctcttcttcc tcacgttccc cgttttctac tacgtttatc tccacgcgac
     1921 gtacggacag acgttcggca agatggcgtt gaagatcaag gtggtgaacg aggacgggac
     1981 tcccctcgat taccggaagg cgttccttcg gtggctgggg tacttcctgt gcgatctgac
     2041 cttctacatc gggtacctgt gggccgcctt cgacccccgc aagcaggggc tgcacgacaa
     2101 ggtttgcaaa accgtcgtcg tgcggaccga cgtgccgttc ccgcttcccg aggcggtgga
     2161 gccggaagcc ccggccgaga ctcctccagg agaaccggct tcccttgaca ctgaggcgag
     2221 cccgccgata taatccgacg ttccgccagg tggcccttta gctcagatgg tagagcagag
     2281 gactgaaaat cctcgtgtcc ccagttcaat tctgggaggg gccaccatga aaatcaggga
     2341 gttagccggc gacggctaac tcttttttta tttaccggga ccatatcggg accaaccggc
     2401 ttacccgcgc ccaacaagga agccatacca gccgtgtcat cgtagtcgcc ggcgaaaaag
     2461 ggcgacggcg ctttcgattt cctttcccgg agctcttaga tccgccctcg tgatcattcg
     2521 aggagcgtca aaagcgttca ccggcgcttc tccgtggctt cccaccgaaa gacttccccc
     2581 tcggggtcgc tctgctcgca gggtcctcat cccgattccg ctcagggtag tgttgtaatg
     2641 catgcgatca aatcgttgat aaggatccct ggacgacggc atcagttgct ccgccatcgg
     2701 gggaaaagaa gacttcggta acctgcgccg ttaaagggcc agcggatatc gattggattg
     2761 gagaggagca agcgaaggtg atcgtgagca ggcaagcgag gacaagaata atgcggaaac
     2821 gagggctacc catcagcgat gtccccaaag ttttgatgcg cacattatcg ggtatttttg
     2881 atgtactata aactaataga cggaatcatc ccccgtgagt cttcctcgct tcgaacctcc
     2941 aaccaaggga ggtcctctgt gagataatcc atctccatta tcgttgcttc tctgctggtc
     3001 gttctcgcat tcacgcaact tttatttgct gatgatgtgt ccgttcgagg gtactgtcgg
     3061 gatagcaatg acacattatg cgcaatcaat taagccgctg tgtaaatcat caagataatt
     3121 gagaattact aatttctaaa ggagaagacg atgtggcaga ttcctattaa gcgtacgctt
     3181 gaccgtattc ctggtggcat gatgatcgta ccccttctgt ttggtgcggt cattaacacg
     3241 tttttcccaa ccacacccaa attctttggg tcctttaccg gtgcgttgtt cactggtgca
     3301 ttgccaattc ttgctgtctt ttacgtctgc atgggtgcaa gcatcgacgt caaggcgact
     3361 ccttacattt tgaaaaaagg tgggtccctg tttgccgtc
//
LOCUS       DASVJL010000083         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1138260, whole genome shotgun sequence.
ACCESSION   DASVJL010000083 DASVJL010000000
VERSION     DASVJL010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138260"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VF357_01560"
     CDS             complement(<1..131)
                     /locus_tag="VF357_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7522860.1"
                     /translation="MGLTDVDLEIGAGEFVAVMGPSGSGKSTLMNILGCLDVPTSGT"
     gene            complement(190..1419)
                     /locus_tag="VF357_01565"
     CDS             complement(190..1419)
                     /locus_tag="VF357_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7522861.1"
                     /translation="MKRHWILLACTAVVVSSGAIAYLRISGKIPVDVFRVRLGRVEEI
                     TAAVSAGTVKSSLEAVLSTETGGRVSEVLVREGSTVRRGDVLARIVDSELPRQTEAAL
                     AEERQARDALLQAEAKRQEAAIRVRAERGRAENNLRKAREDHRRTEELFLDGIVSRSD
                     RDTAATLLGNAEEEARYAAAGGSMLQALDREIDVLRGRIEAARARAAALTARGSKLTI
                     TAPFSGVITKKTCEIGESKLPGGALLTLADPSSSYVEAQIDEADAAKVRVGQTARLVP
                     EAFRGEKFTGRVSEVRPTVEASKEVSRANTIAITLTSAPQPLRLGMSVDVEVLTGGKD
                     NVPLIPSAALMERDAKKFVYVVSGGKAVRRDVTVGISNWDRTEILTGVSPGEDVVTSL
                     EIKDLAPGSHVEIRSRR"
BASE COUNT          234 a          576 c          495 g          293 t
ORIGIN      
        1 taggtccccg acgtcgggac gtcgaggcag cccaggatgt tcatgagggt agattttccc
       61 gaaccggagg ggcccatcac ggcgacgaac tccccggcgc cgatctcgag gtccacgtcg
      121 gtcaggccca cgacccgccc ggcgcccgcc tcgtaaagtt tcgtcacccc acgcagtcgg
      181 aggaggggct caccggcggc ttcggatctc aacgtggctc ccgggtgcga ggtctttgat
      241 ctccagcgag gtgaccacgt cctctccggg ggacaccccc gtgagaatct ccgttcggtc
      301 ccagttcgaa atccccaccg tcacgtcgcg tcggacggct ttccctccgg agacgacgta
      361 gacgaacttt ttcgcgtccc gctccatcaa ggcggcggac gggatcagtg gaacgttgtc
      421 tttcccgccc gtgagaacct cgacgtccac cgacattccg agacggagcg gttggggggc
      481 ggaggtgagc gtgatggcga tcgtgttggc ccgcgagacc tctttggacg cttccaccgt
      541 cggtcggacc tcggagaccc gaccggtgaa tttctctccc cggaacgcct cgggcaccaa
      601 ccgggccgtc tgtccgaccc ggaccttggc ggcgtcggcc tcgtcgatct gcgcctccac
      661 gtaggaagac gaaggatcgg cgagggtcag cagcgcccct cccggcaact tgctctcccc
      721 gatctcgcag gtcttcttcg tgatcacgcc ggaaaacggc gccgtgatcg tcagtttcga
      781 cccgcgggcc gtcaaggcgg ctgcccgcgc ccgcgccgcc tcgatcctcc cgcggaggac
      841 gtcgatctcg cggtccaggg cctggagcat tgaccccccg gccgcggcgt accgggcttc
      901 ttcttccgcg ttgccgagca gcgtcgcggc ggtatcccga tcggagcggg agacgattcc
      961 gtccaggaac agttcctccg tccgccggtg gtcctcgcgc gccttccgga ggttgttctc
     1021 cgcccttcct ctttccgcgc ggacccggat ggccgcctcc tgccgcttcg cctccgcctg
     1081 gagaagggcg tcccgcgcct gtctctcctc ggcgagtgca gcctcggttt gccgcggcag
     1141 ctcggagtcg acgatccggg cgaggacgtc gcctcgccgc accgtggaac cttcccggac
     1201 gagcacctcg gacacccggc cccccgtttc ggtggagagg accgcctcga gggacgattt
     1261 cacggttccc gccgagaccg ccgcggtgat ctcctcgacg cgcccgagcc ggacccggaa
     1321 gacgtccacc ggaatcttgc cggagatcct gagatacgcg atcgctccgg acgagacgac
     1381 caccgcagtg catgcaagga ggatccaatg ccgtttcata gggacctaag tgttgcgttt
     1441 tataaatacg gcaacatata actgttgtgt actcggactc cggtctcgcg ggggggcttc
     1501 ctctccgcta cggctcgtcg accgtccatg gcctcctcgc ctgcgcctcc gcttgctccc
     1561 gacatccctg tctccgctgc gctccagacg ccgcttcg
//
LOCUS       DASVJL010000084         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_293875, whole genome shotgun sequence.
ACCESSION   DASVJL010000084 DASVJL010000000
VERSION     DASVJL010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293875"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..417
                     /locus_tag="VF357_01570"
     CDS             142..417
                     /locus_tag="VF357_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522862.1"
                     /translation="MERSRTHAQRRILAGSGAGRALSALLLALYLLLSLPSSAATDNS
                     KAGTYEDRATAEKMSRSRYRDLEVASLVVILVAGGAAILWAVRRRKP"
     gene            419..>1503
                     /locus_tag="VF357_01575"
     CDS             419..>1503
                     /locus_tag="VF357_01575"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7522863.1"
                     /translation="MNRNPLKRILVVDDEPMIVSLLSTILREKGWDVTEAGSGTDGID
                     HLDRARFDVIVTDLVMPGDSGIDLLRAAKEIHPDVEVILMTGYATADTAIEAMRNGAF
                     HYIMKPLKPEEVMNLVEKAYSQRQLQRENQFLKSEIRAGHHVQSVVGDSEPILRLIAT
                     LQGIAGVDSPVLLTGERGTGRGFFARIVHFHSRRSAELCVPVYCAGVPEETLIAELFG
                     GPCAIEDQTSVPHSGKMELANHGTLYLSDFAEAGPEVLGRIDRLLADKAMVPDGGTEP
                     IALDVRIIASSAVPVEELARGGNVPPTLLKSMEPGIVRIPTLREHLEDVPLLLHHFLQ
                     EANRERKKPLRGFTSSALSALETYDWPG"
BASE COUNT          251 a          511 c          506 g          235 t
ORIGIN      
        1 tggggcggag gacacggctt gcccggagtg ccgtgcgacc gtcgtccgcc gggagggata
       61 cggcgtcgat gcgcacggcc tgtccgggaa ccggtgcgcc gcctgcggcg ctcgcctgcg
      121 attcgtggta taaaccaacc gatggaacgt tcgcgcacgc acgcccagcg taggatcctc
      181 gccggttcgg gggcggggag ggctctctcc gctctccttc tcgccctcta cctgctcctc
      241 tccctgccat cctccgccgc gaccgacaac agcaaggcgg gaacgtacga ggaccgcgcg
      301 accgcggaga agatgagccg atcgcgctac cgggacctcg aggtggcctc cctggtcgtc
      361 atcctcgtcg cgggcggggc cgccatcctc tgggcggttc gcaggaggaa accttgacat
      421 gaaccggaat cccttgaagc ggatcctggt cgtcgacgac gagccgatga tcgtctccct
      481 cctctccacg atcctccggg agaagggatg ggacgtcacc gaggcggggt ccggaacgga
      541 cgggatcgac catttggacc gggcccggtt cgacgtgatc gtcaccgacc tcgtcatgcc
      601 gggggacagc ggcatcgacc tgctgcgggc ggccaaggag atccaccccg acgtcgaggt
      661 gatcctcatg acggggtacg ccaccgccga taccgccatc gaggcgatgc gcaacggcgc
      721 cttccactac atcatgaagc cgctgaagcc cgaggaggtg atgaacctcg tcgagaaggc
      781 gtactcccag cggcaactgc agcgggaaaa ccagttcctg aaatcggaaa tccgcgccgg
      841 ccaccatgtt cagtccgtcg tcggcgacag cgaacccatc ctgcggctga tcgccaccct
      901 ccaggggatc gcgggcgtgg acagcccggt gctgctcacc ggggagcgcg gcaccgggcg
      961 cggcttcttc gcccgcatcg tgcacttcca cagccggcgg tccgcggaac tgtgcgtccc
     1021 ggtctactgc gcgggcgttc cggaagagac gctcatcgcg gagctgttcg ggggcccgtg
     1081 cgccatcgaa gatcagacgt ccgtccccca ttccggaaag atggagctcg cgaaccacgg
     1141 cacgctctac ctgtccgact tcgccgaggc gggaccggag gtcctcggga ggatcgaccg
     1201 gctccttgcc gacaaggcga tggtgccgga cggtggtacg gaaccgattg cgctggacgt
     1261 ccggatcatc gcttccagcg cggtccccgt ggaggaactg gcgcgggggg ggaacgttcc
     1321 gccgacgctg ctcaagtcga tggagcccgg gatcgtgcgc atccccacgc tgcgggagca
     1381 cctcgaggac gtgccgctgc tgctgcacca tttcctgcag gaggcgaacc gggagcggaa
     1441 gaaaccgctc cgtgggttca cttcctccgc gctctccgct ctcgaaacgt acgactggcc
     1501 ggg
//
LOCUS       DASVJL010000085         8500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1795575, whole genome shotgun sequence.
ACCESSION   DASVJL010000085 DASVJL010000000
VERSION     DASVJL010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795575"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="VF357_01580"
     CDS             complement(<1..169)
                     /locus_tag="VF357_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX7522864.1"
                     /translation="MNRGEAFPGIVLVGFMGSGKSSVGRELARRFGVPFVDVDEWIES
                     AAGCRIRDLFAR"
     gene            complement(166..1359)
                     /gene="aroC"
                     /locus_tag="VF357_01585"
     CDS             complement(166..1359)
                     /gene="aroC"
                     /locus_tag="VF357_01585"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX7522865.1"
                     /translation="MAHFSFRTAGETHGPGLVTIVDGVPAGLPVRAEEINRELARRQV
                     GYGRGERMQIEKDEVEILSGVRFGKALGSPVALLLRNRDWVNWREKMAQDGDGEGIPK
                     LDTARPGHADLPGILKYGHGDVRNVLERASARETAARVMAGALAKAILRDLGVDIVGH
                     VLSIGMYRVSAGIEGNRIAAARAEGSPLRMADPAVEAEVKRWIDGLKEAGTTAGGVVE
                     VIAGGVPPGLGSYVAWDRRLDGRLGQALMCIPAIKGVEIGGGVGLAGMPGIDVHDEVF
                     PGGNPAALYLGKFLLPFHRDTNRAGGLEGGMTNGEPVVVRAAMKPIPTQSVPLRTVTV
                     DRFVSSTAHRERSDVCAVPAASVVAETMVAIVLADAFLEKFGGDAMRDILYNYSGYLR
                     RICGE"
     gene            complement(1437..3776)
                     /gene="pilQ"
                     /locus_tag="VF357_01590"
     CDS             complement(1437..3776)
                     /gene="pilQ"
                     /locus_tag="VF357_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX7522866.1"
                     /translation="MMTPLRHSGRVLWPRVLRGMAAMALACAILVPVAIAAEPAGPDA
                     AQPAGAPGAKIREVTVSKSPYNTTIQASVDGAIENYNSFKLNDPFRIVVDVWGVGQGT
                     AASEIPVGTPEVKVVKISSMDRKLRMVVETPGDRPMPFIVSAEKGVLVLSVGGGAEEK
                     ITSTERLQDGKAPMKGPVVVGIDLEDLPDASNVVIATAGDPPYQVLKKAGGVTLVFKG
                     AGAEKGLLRRIDARKFDIPVKAITPSGGKKGVTVAVAFAPGSRYTVEKRDGSVVVAFP
                     KGAADGKTNLVARAAPGRAPRMAKADVEPEPLDANAEPASLEGSQSWGFLTGSSDVGR
                     KYHGQRISMDFKDADLTNVFRIIAEVSNLNIITADDVKGKVSLRLVNVPWDQALDIVL
                     RSKSLGASQEGNVLRIAPLSSLRKEEQDRFDAQKQIELSRQEALNRAAEVKASQEAVF
                     DTIPISYGKASEMLVKIKPLASKFGRLDSDDRTNVLIIRDLQQNIAEVRALVATLDTA
                     TPQVLIEARIVEVDTTFSRELGVQWGGSFREGRGATKYGMTGAQDATGAAIPGGAVLA
                     ATTNPFSAAAPVPSYAVNLPAAIGLGSGGGIAFGILRDNLRLDLSLSALEASGKGKII
                     SSPKIVTTDNKEATIEQGTQIPYSTVSASGTNVQFIDATLSLKVTPHITPDGRVAMKL
                     EAKNDSQGDVAPGSTTPSINKKKATTEVLIRDGETTVIGGILQITRNESRSGLPWLSK
                     IPVLGYLFRKDTNSTQNRELLIFITPKILMQEPIQAKAS"
     gene            complement(3776..4345)
                     /locus_tag="VF357_01595"
     CDS             complement(3776..4345)
                     /locus_tag="VF357_01595"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEX7522867.1"
                     /translation="MRPPPVRHVSLAVGILAAVTLFAGGFGCSKEEPAAPQPVVKRQA
                     PKPEAKAPEAKAPEAKAVEAQAKKAEPVAPYDSRGKRDPFVSFIKVEERKRVGIDTKA
                     LPPLQRYDLGELKFVGVIWTKKGALGLVEDAEGKGYSVTVGTRIGRSGGVVSRITGKE
                     ILVKEEFVGNRGEKILKESGIQLTTAGGN"
     gene            complement(4342..4959)
                     /locus_tag="VF357_01600"
     CDS             complement(4342..4959)
                     /locus_tag="VF357_01600"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX7522868.1"
                     /translation="MALKLGDLSKVPPRQKVLLAVLFCGLVISGYYYLYYREASQRIG
                     VMETELAGLQSKIREQQAIAGNLRSFQDEVRRLETQLSFLLEQLPNSAEIPSLLKSVS
                     DLGKESGLDFLRFAPSGEIKKDFYAEIPVSISVNGDYHNFALFSDKVARYPRIVNLSN
                     ITFSSPKPSGDNVVLVTVNCTATTYRFLELQAAATPGAGDQGKKQ"
     gene            complement(4988..5521)
                     /locus_tag="VF357_01605"
     CDS             complement(4988..5521)
                     /locus_tag="VF357_01605"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX7522869.1"
                     /translation="MIRINLVRGKRKKRRELNVGSAWIALPLVVLAGTIYFHTTVTGR
                     ISKLDADIGKANSDIARLKKEIGEVEKFKARKAELQKKVDIISNLQKGRTGPVRYFEA
                     LSAAIPEKCWIDTLGVKDEMVSLSGVALNNYTIANFMTALGQTGRFRDVVLGAAEQTT
                     VAGVKLVRFNLTFQTVN"
     gene            complement(5518..6579)
                     /gene="pilM"
                     /locus_tag="VF357_01610"
     CDS             complement(5518..6579)
                     /gene="pilM"
                     /locus_tag="VF357_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX7522870.1"
                     /translation="MGLFGSKELIGLDIGSSSVKMAHVKAVGAENRLRKFGVFPLPAD
                     AIVDGAIMDHGAVVEGIKTALRDLKIHEKEVAISLSGHSVIIKKVQLPTTTAEELEES
                     IQWEVEQYIPFDIKDVKIDFQVIGPLKDDPSKMDVLLVAAKTDLISDYMSVVKDAGLV
                     PRIVDIDSLAAGNAFELTHPVSDDQVPMVVNVGASFMNINILHAGVPLFTRDVPMGGG
                     MYTTEIQKQVAVSFETAEEYKTGKKDPGERAEKVTEIMKVVSSLLATEAQRSCNFFSA
                     TYPDRLVTKVYLTGGASRSAFLKEMLAEKIGVDVEIFDPFEGLAVDDKAVDPSVVAQL
                     NTSATVSIGLALRNLEDRG"
     gene            complement(6602..6799)
                     /locus_tag="VF357_01615"
     CDS             complement(6602..6799)
                     /locus_tag="VF357_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522871.1"
                     /translation="MPNNVRKVREGLLLSKAELARRAKISVLTIDRVEKGMACRMDTK
                     RKIILSLGLKPSDRDKVFSKE"
     gene            complement(6983..8374)
                     /locus_tag="VF357_01620"
     CDS             complement(6983..8374)
                     /locus_tag="VF357_01620"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7522872.1"
                     /translation="MADRILIVDDEASLRDALARILTAEGYEVRQAGDGKEALGILGA
                     SPFDFLLCDLRMPVMGGLDLLREITARGIPGTVIMMSAFGTVETAVEAMKLGAYDYVS
                     KPFMSDEILLTLRKAAERERLRTENAFLRSEIEKTARPEEILYASGPMEEVVRTAGKV
                     KDYDATVLVTGESGTGKELVARLLHFGGRRKGKPFVAVNCGAIPETLLESELFGHRRG
                     AFTEAKSDRAGLIEEAGGGTLFLDEIGELPLPLQTKLLRFLQEGEIRRLGDTETRKVN
                     VRLVAATARDLEREVAGGRFREDLFYRLNVIRIHVPPLRERRDDIPLLAKHFLSHYCG
                     KYGKREMLLSPDALAAMTAHEWRGNVRELRNLMERCTLLATGTEVTRAGLLAVWKGSV
                     GGGETGPAFEIRVPVSPERPDLKAAVRELERQLIRIALERTGWSRPKAAELLGISHPT
                     LLYKAKEFGIEGG"
BASE COUNT         1388 a         2969 c         2518 g         1625 t
ORIGIN      
        1 cccgggcgaa cagatcccgg atcctgcacc cggccgccga ctcgatccat tcgtccacgt
       61 cgacgaacgg cacgccgaac cgtcgcgcca gctcccggcc gacggagctt ttccccgacc
      121 ccatgaaccc gaccagcacg atccccggaa acgcctcgcc gcggttcatt ccccgcagat
      181 gcgccgaagg tacccggaat aattatagag gatatcccgc atcgcatcgc ccccgaattt
      241 ctcgagaaat gcgtctgcca gaacgatcgc caccatcgtc tccgcgacca cggaggcggc
      301 ggggacggcg cagacgtcgc tgcgctcgcg atgcgcagtc gaggagacga accggtcgac
      361 cgtgacggtc cggagaggga ccgactgcgt cgggatcggc ttcatcgcgg cccggacgac
      421 cacgggttcc ccgttggtca tccccccttc cagccccccg gcacggttcg tgtcccggtg
      481 gaacggcagc agaaatttgc cgagatacag cgccgccggg tttccaccgg ggaacacttc
      541 gtcgtggacg tcgatccccg gcatcccggc cagtcccacc ccgccgccga tctccacccc
      601 cttgatggcg gggatgcaca tcagcgcctg tccgaggcgg ccatcgagac gccgatccca
      661 ggcgacatag gacccgagac ccggaggaac tcctcctgcg atgacctcga cgaccccgcc
      721 ggccgtggtg ccggcctcct tcaacccgtc gatccaccgc ttgacctcgg cctcgactgc
      781 cgggtccgcc atccgtagcg gactcccctc ggcgcgcgcg gccgcgatgc ggtttccctc
      841 gatcccggcc gatacccgat acattccgat cgagagaacg tggccgacga tgtcgactcc
      901 gagatcgcgg aggatcgcct tggcaagcgc accggccatg acccgcgccg cggtctccct
      961 cgcgctggcg cgctccagga cgttccgtac atctccgtgc ccgtatttca ggatgccggg
     1021 aaggtccgcg tgcccggggc gggcggtgtc gagcttcggt atcccttccc cgtccccgtc
     1081 ctgggccatc ttctcccgcc agttgaccca atcgcggttg cgcaggagca acgcgacggg
     1141 gctccccaac gcctttccga aacggacgcc cgagaggatc tcgacctcgt ccttctcgat
     1201 ctgcatccgt tctccgcggc cgtaccccac ctgccgccgg gctaactccc ggttgatctc
     1261 ctccgcccga acgggaaggc ccgccgggac cccgtcgacg atcgtgacca ggcccgggcc
     1321 gtgggtctcg ccagcggtcc ggaacgagaa gtgcgcgatg gcctaccctc cttgaatgaa
     1381 aaaaggcacc cggacggaac cgcccaggtg cccgatggtt gccgcccgga ccgggttcag
     1441 gaagccttgg cctggatcgg ctcctgcatc aggatcttcg gcgtgatgaa gatcagcaac
     1501 tcccgatttt gggtcgagtt cgtgtccttc cggaacagat acccgaggac ggggatcttc
     1561 gagagccatg gcagcccgga tcggctttcg ttacgcgtga tctgcaagat cccgccgatg
     1621 accgtcgtct cgccgtcgcg tatcaggacc tccgtggtcg ccttcttctt gttgatcgaa
     1681 ggggtggttg atccaggagc cacatcgccc tgagagtcgt tcttcgcctc gagtttcatc
     1741 gcgacccgcc cgtcgggcgt gatgtgcggc gtcaccttca ggctaagggt ggcgtcgata
     1801 aactgcacgt tcgttcccga agcggacacg gtggagtacg ggatctgcgt cccctgttcg
     1861 atggtggctt ccttgttgtc ggtcgtcacg atcttcggcg aagagatgat cttccccttc
     1921 ccggacgcct cgagggcgga gagcgaaagg tcgaggcgaa ggttgtccct caggatcccg
     1981 aatgcgatcc cgccaccgga ccccaggccg atcgccgccg ggaggttcac ggcatagctc
     2041 ggaacggggg cggcggcgga gaagggattg gtggtcgccg caaggaccgc ccccccggga
     2101 atggccgcgc cggtcgcgtc ctgcgccccg gtcatcccgt atttggtggc tccgcggccc
     2161 tcccgaaagg aaccgcccca ctggacgccg agttcccggg agaacgtggt gtccacttcg
     2221 acgatgcgcg cctcgatgag aacctgcggt gtcgccgtgt cgagcgtggc aaccagtgcc
     2281 ctcacctcgg cgatgttctg ttgaagatcg cggatgatga gcacgttcgt ccggtcgtcg
     2341 ctatccagtc tcccgaactt cgaggcgagc ggcttgatct tcacgagcat ctcggacgcc
     2401 ttgccgtagc tgatcgggat cgtgtcgaag accgcctcct gcgacgcctt tacctcggcg
     2461 gcccggttca gcgcctcctg acgcgacagc tcgatctgct tctgcgcatc gaaccggtcc
     2521 tgctcctcct tccgaagcga ggaaagcggc gcgatccgga ggacgtttcc ttcctgcgac
     2581 gcgccgagcg acttcgagcg gaggacgatg tcgagcgcct gatcccacgg aacgttgacc
     2641 agccggaggg agaccttccc cttgacgtcg tccgccgtga tgatgttcag gttgctgacc
     2701 tcggcgatga tccggaaaac gttggtgagg tcggcatcct tgaagtccat ggagatccgc
     2761 tgcccgtgat atttccggcc gacgtcggaa ctcccggtga ggaatcccca cgactgcgaa
     2821 ccctcgaggg acgccggttc cgcattcgcg tccaagggct ccggttccac gtccgccttc
     2881 gccatgcgcg gggccctccc cggcgccgcg cgcgcgacca ggttggtctt accgtcggcc
     2941 gcaccctttg gaaacgccac cacgaccgag ccgtccctct tttctacggt gtaccgggaa
     3001 cccggcgcga acgcgacggc gaccgtaacc ccctttttcc cgccggaggg cgtgatcgcc
     3061 ttcacgggga tatcgaactt tctcgcgtcg atccgccgga gcaacccctt ctccgcgccg
     3121 gcgcccttga acacgagcgt cacgcctccc gccttcttca agacctgata gggcgggtcc
     3181 cccgccgtgg cgattacgac gttcgacgcg tcgggcaggt cttcaaggtc gatcccgaca
     3241 acgacgggac ctttcatcgg tgccttcccg tcctggagac gctccgtgct ggtgattttc
     3301 tcttccgcgc cgccaccgac ggacaggacg agcacgccct tctcggcgct gacgatgaac
     3361 ggcatcggcc ggtcgccagg cgtctccacc accatccgca acttccggtc catggaggag
     3421 atcttcacca ccttgacctc gggggtcccc accgggatct cggatgccgc cgtcccctgg
     3481 cccactcccc agacgtccac cacgatccgg aacgggtcgt tcagcttgaa ggagttgtag
     3541 ttctcgatcg ccccgtcgac agacgcctgg atcgtcgtgt tgtaaggcga cttcgatacc
     3601 gtcacctccc tgatcttggc gccgggagcc cccgcgggct gcgcggcgtc cgggccggca
     3661 ggttccgctg cgatcgcgac cggcacgagg atcgcacagg caagcgccat cgccgccatc
     3721 ccccggagga cgcggggcca gagaactctg cccgaatgcc gtaacggggt catcattaat
     3781 ttcctcctgc cgtggtgagc tggattccgc tttccttcaa gattttttcc cctcggttgc
     3841 cgacgaactc ttccttcacg agaatctcct tcccggtgat ccggctgacg acgccgccgg
     3901 agcggccgat ccgcgttccc accgtcacgg agtacccctt cccctcggca tcctccacaa
     3961 ggcccagcgc gccctttttc gtccagatga cgccgacgaa cttcaactcg cccaggtcgt
     4021 accgctgcag gggagggagc gccttcgtgt cgatccccac cctcttgcgc tcctcgacct
     4081 tgatgaacga cacgaacgga tcccgcttcc cgcgggagtc gtagggggcg accggctcgg
     4141 ccttcttcgc ttgcgcctcc accgccttcg cttccggggc cttcgcttcc ggggccttcg
     4201 cctcgggctt cggcgcctgc ctcttcacca ccggctgcgg cgccgccggt tcctccttgg
     4261 agcagccgaa cccgcccgcg aagagggtaa ccgcggcgag gatcccaacg gccagggaga
     4321 catgtcgcac gggagggggc ctcattgttt cttcccctga tcccccgccc cgggagtggc
     4381 ggccgcctgc agttcgagga agcggtaggt cgtggccgtg cagttgacgg tcaccaggac
     4441 gacgttgtct cctgacggtt ttggagagga aaaggtgatg ttggacaggt tcacgatccg
     4501 ggggtagcgc gccaccttgt cggagaagag cgcgaagttg tgatagtcgc cgttgaccga
     4561 gatagatacg gggatttcgg cgtagaaatc cttcttgatc tcgccggacg gagcgaagcg
     4621 aaggaagtcg agccccgatt ccttcccgag atcggaaacg ctcttcagca gggaggggat
     4681 ctccgccgag ttcggaagct gctcgagcag gaacgacagc tgcgtctcga gccgccggac
     4741 ctcgtcctgg aaggaacgca ggttcccggc aatcgcctgc tgctcccgga tcttgctctg
     4801 aaggcccgcc agctcggtct ccatcacgcc gatccgctgc gacgcctccc ggtaatacag
     4861 gtagtagtac cccgagatga cgaggccgca gaacaggacg gcaagcagaa ccttttgccg
     4921 gggcggcacc ttcgaaagat cgccaagttt tagcgccatt ccgtatcctc tcccggtgat
     4981 gggccgttca gttgaccgtc tggaacgtca agttgaacct tacgagtttc actcccgcca
     5041 ccgtcgtctg ctcggcggcc cccagcacga cgtcccggaa gcggccggtc tgcccgaggg
     5101 cggtcatgaa gttcgcgatc gtatagttgt tcaaggcgac gccggaaagg ctgaccattt
     5161 cgtctttcac gcccaacgta tcgatccagc acttctcggg gatcgcggcc gacagcgcct
     5221 cgaagtagcg gacgggcccg gtacgcccct tctggaggtt ggagatgatg tccacctttt
     5281 tctggagctc cgctttccgg gccttgaact tctcaacctc gccgatctct tttttcaggc
     5341 gcgcgatgtc ggagttggcc ttcccgatat ccgcgtcgag cttcgagatt cttcccgtga
     5401 ccgtagtgtg gaagtagatc gtcccggcca ggaccaccag cggcaacgca atccaggcgg
     5461 acccgacgtt cagctccctg cgcttcttcc ttttcccccg gacgagattg atccggatca
     5521 tccgcgatcc tccaggttcc gcagcgcgag accgatcgac accgttgcgg aggtgttgag
     5581 ttgggcaacc acggacggat cgacggcctt gtcgtccacc gccaacccct cgaacggatc
     5641 gaagatctcc acatcgaccc cgattttttc cgccagcatt tccttcagga acgcagacct
     5701 ggacgcccct ccggtcaggt acactttcgt caccaggcgg tcggggtagg tcgcggagaa
     5761 gaagttgcag gaccgttgcg cctcggtcgc aagaaggctg gaaacgacct tcatgatctc
     5821 agtgaccttc tcggcgcgct ccccggggtc cttctttccc gtcttgtact cttccgccgt
     5881 ctcgaagctg acggcgacct gcttctggat ctcggtggtg tacatccctc cgcccatcgg
     5941 gacgtcccgc gtgaagaggg gaactccggc atgcaggatg ttgatgttca tgaacgacgc
     6001 tccgacgttg acgaccatcg gcacctggtc gtcggaaacc gggtgggtaa gctcgaaagc
     6061 gttcccggcg gccagcgagt cgatgtcgac gatccggggg acgaggccgg catccttcac
     6121 gacggacatg tagtcgctga tcagatcggt tttcgccgcc accaggagga cgtccatctt
     6181 ggaagggtcg tccttcagcg gaccgatcac ctggaagtcg atcttgacgt ccttgatgtc
     6241 gaaggggatg tactgctcca cctcccactg gatcgactcc tcgagctcct cggcggtcgt
     6301 cgtgggcagt tgcaccttct tgatgatcac ggagtgcccg gagagggaga tggcgacttc
     6361 cttctcatgg atcttcaaat cccgcaaggc ggtcttgatc ccctcgacga cggcgccgtg
     6421 gtccatgatc gccccgtcga cgatcgcgtc ggccggaagg gggaagaccc cgaacttccg
     6481 cagacggttc tcggcgccca ccgccttgac gtgggccatc ttgacggaac tcgacccgat
     6541 gtccagtcca atgagctctt tactgccgaa cagccccatc gacgcccccg tgatcccgcc
     6601 gctattcctt gctgaacacc ttgtcccggt cggacggctt caagcccagc gacaggatga
     6661 tcttgcgctt cgtgtccatg cggcacgcca tccccttttc cacccggtcg atcgtgagga
     6721 ccgagatctt tgcgcgccgc gccagttccg ccttgcttaa caggagccct tccctcactt
     6781 ttcggacgtt gttgggcaca actttaacat tttctttttc ggatcttact ttttctttat
     6841 cggggctacc cacggttttc gactcctccg acatctccct cgttcgacgc cgaatgacat
     6901 catccgtgaa aaagtatagc ccacgcatat tacgtgtcaa gaaatattca agtgacatcc
     6961 aatgacgcat acttatgcga tattatcccc cctcgatccc gaactccttc gccttgtaca
     7021 ggagcgtcgg gtggctgatc ccgagcagtt cggccgcctt gggccggctc catccggtcc
     7081 gctccagggc gatcctgatc agttggcgct cgagctcccg aaccgccgcc ttgagatccg
     7141 gccgctccgg ggagacgggg acccggatct cgaaggccgg tccggtctcc cccccgccaa
     7201 cgcttccctt ccacaccgcg agcaacccgg cgcgtgtaac ctccgtgccc gtcgccagga
     7261 gtgtacatcg ctccatcaag ttgcgaagtt cgcggacgtt cccccgccac tcgtgggcgg
     7321 tcatcgccgc aagagcgtcc ggcgacagga gcatctcgcg cttcccgtac ttcccgcaat
     7381 agtgggacag gaagtgcttc gcgaggagcg gaatatcgtc gcggcgctcc ctcagggggg
     7441 gcacatggat ccggatcacg ttgaggcgat agaagaggtc ctcgcggaac cgtcccccgg
     7501 ccacctcgcg ctcgaggtcg cgcgccgtcg cggccaccag gcgcacgttg accttccttg
     7561 tctccgtgtc gccgaggcgg cggatctccc cttcctgaag aaaacggagc agcttcgtct
     7621 gcagcggcag ggggagctcc ccgatctcgt caagaaagag cgtgccgccg ccggcctcct
     7681 cgatcaggcc ggcccggtcg gatttcgcct cggtgaaggc tccccggcgg tggccgaaga
     7741 gctcgctctc gagaagcgtt tccgggatcg ccccgcagtt gacggcgacg aacggcttcc
     7801 ctttccgacg ccccccgaag tgaaggagtc gcgccaccag ttccttcccg gtgccgctct
     7861 ccccggtgac cagcaccgtg gcgtcgtaat ccttcacctt gcccgccgtc cggaccacct
     7921 cttccatcgg gccggaggcg tagaggatct cctccgggcg ggcggtcttc tcgatctcgc
     7981 tccgcaggaa ggcgttctcg gtccgaagcc gctcccgctc cgccgccttc cgcagggtga
     8041 gaaggatctc gtcgctcatg aacggcttcg aaacatagtc gtaggcgccc agcttcatcg
     8101 cctcgacggc ggtttccacg gtgccgaacg ccgacatcat gatgacggtc cccgggatcc
     8161 cgcgcgcggt gatctcccgc aggaggtcga gaccgcccat cacgggcatc cgcaggtcgc
     8221 acaggaggaa gtcgaacggc gatgcgccga ggatcccgag cgcttccttc ccgtctcccg
     8281 cctgccggac ctcgtaccct tccgcggtca ggattctcgc cagcgcgtcc cgcagcgacg
     8341 cctcgtcgtc cacgatcagg atccggtcag ccatggcttc tcttttctcc cctgctcccc
     8401 gccgttccgc gggcggacgg cagcaccacg aggaaggtcg cccccttccc cccctcgctc
     8461 tccgcacgga tctccccccc cgccccctcg acgatcgtcc
//
LOCUS       DASVJL010000086         3542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_200942, whole genome shotgun sequence.
ACCESSION   DASVJL010000086 DASVJL010000000
VERSION     DASVJL010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200942"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..2829
                     /locus_tag="VF357_01625"
     CDS             52..2829
                     /locus_tag="VF357_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522873.1"
                     /translation="MTALTRLFDIEDLSLTDARLRVAIPQGDLSVDGLRLSLAPGNGE
                     TRAFGGSGDLSFRGKGSPVFAAKLSARGTVTPEPALTVDLEGTSGHLELPWISGNLSG
                     RTRLRMTRKNLRIEDLTLTLPQGRVNLVPRGAIITEPIRLNVAAGVTLDGRDPLLEVR
                     GLDIGGLLIARGRLSGPILERMSGTLEGEIPRVERVSTYWAPLLPGTLAHMELKGRLP
                     WRLSLSTGITERVLALDLLPPDLVLSGAIAGLECRFGGSVKVAGSLDGWLHGRTTLSG
                     RVQGTGHFDRPPLAVRHFRFDTPLAGAMTAPTLPGWILSAGPGEVLYEGRPLPLGTLA
                     IRGSARPVGNSYRVEGVEIRSGSLGRLTGQVAFHGGNVSGALDEVRLPADNLVSLAQA
                     VSGREWNGWSPTGAIDVAARLETVEAGPRLAATAVLGRIGFTSPAGDIMGQNLAGKVD
                     LDALLIRNPRMNADLVLRGGEALWGTVYVDLAKNPVDLHAGGTRAGPGEYKNIVLEGG
                     LEGFGRLGIEGKALLAGETWRHQGHFVLRDARLGPIFRTFLRDPLVTSHPDLAGLETE
                     GTAGIDLSFSGSGKAAALDGTLRLRSGDLHRGAEPPLLSGLNIDLPISYSFGAANPGV
                     TAPSDAAKWGRLRLEKIRLSGQELGPLDMPAVLVPNHLYLGGTVEASLFGAKLALRRI
                     QVDEPLSPGFRIRMAAKLDDLNLSRIAGKNPMLEGRLGGILDPVTIGQERMDADGELT
                     GDLFGGRMDVRRMMVERPFSAGREIGADVNVDRIDLERFSAALGIGRITGRLSGSITD
                     LRVAYGQPVAFHIKMESVPAKGVSQSVSLKAVNSISLVSTGSALSGMGISLMTTFFRE
                     FPYEKIGFESGLKNDVFTVRGLIHEDGVEYLVKRRFFAGIDVINRNTDNHIGFSDMLE
                     RAKRVTGERSN"
     gene            2868..3482
                     /locus_tag="VF357_01630"
     CDS             2868..3482
                     /locus_tag="VF357_01630"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HEX7522874.1"
                     /translation="MERPAHRIAFSVLAVLALIAGCVTVNVYFPAAQVEKTAQKIVDE
                     VYQEKKEPPAQEPTEKPRSSNDGDTIRGIVRLARFGPAPAFAEEATTVSNAAIRGLKE
                     QIGQRHQELLPFYQQGQVGITRDGFLEVRGTSGLGLPQVAVLKRLVDADNAARRQLYE
                     EVAKALNLKPEQVSQVRKIFAKQWRDKAQSGWTVQSDDGQWGRK"
BASE COUNT          628 a         1109 c         1200 g          605 t
ORIGIN      
        1 ggggtgatta tcgaacgccc tcgcctccgg gagacggaag gcccaccgga cttgacggcg
       61 cttacccgtc tgttcgacat cgaagacctg tcgctgacgg acgcccggct ccgcgtggcc
      121 atcccccaag gagatctttc cgtcgacggg ttgcggttga gcctggcccc ggggaacggg
      181 gagacccgcg ccttcggcgg atccggggat ctctcctttc gcggaaaggg aagccccgtc
      241 ttcgccgcga agctttcggc ccgcgggacc gtcacgcccg agcccgcgct tacggtcgac
      301 ctcgagggga cctcgggtca cctggaactc ccctggattt ccgggaatct ttccggccgt
      361 acccgcctgc gcatgacgcg gaagaatctc cggatcgagg atctgaccct gactctgccg
      421 cagggacggg tgaacctggt accgcgcggg gcgatcatca cggaaccgat ccgcctgaac
      481 gtagcggcgg gggtgacgct cgatggaagg gaccctctcc tggaggtgcg cggattggac
      541 atcggcggcc tgctcatcgc acgggggcga ctgagcggcc cgatcctcga aaggatgtcc
      601 gggacccttg aaggggagat cccgcgggtg gaacgggtga gtacctattg ggctcccctc
      661 ctgcccggta ctcttgcaca catggagttg aagggcagac ttccatggcg cctctccctg
      721 tccaccggaa tcacggaacg ggtcctggcc ctcgatctcc tccccccgga cctggtgctt
      781 tccggggcaa tcgcgggact tgaatgccgc ttcggcggat ccgtcaaggt ggcggggtcc
      841 ctcgatgggt ggcttcacgg caggaccacc ctgagcggtc gggtccaggg aacggggcat
      901 ttcgaccggc cgccgctggc ggtgcgccac tttcgcttcg acactccatt ggccggagcg
      961 atgaccgctc ccaccctgcc ggggtggatc ctctcggcgg ggccgggaga agttctttac
     1021 gaaggccgcc ccctgcccct gggaacgctg gcaatccggg gatccgcaag gccggtcggg
     1081 aattcatacc gtgtggaagg cgtggagatc cgatccgggt ccctcggccg cttgaccggt
     1141 caggttgcct tccacggagg gaacgtcagc ggcgccctgg acgaggtgag actgcccgcg
     1201 gacaacctgg tatccctcgc acaggcggtg agtggaaggg aatggaacgg ctggtcgccc
     1261 acgggcgcga tcgacgtcgc cgcccggctc gaaaccgtgg aggccggtcc ccggttggcg
     1321 gctacggcgg tgctcggacg aatcggcttc acctcgcccg caggcgatat tatgggccag
     1381 aacctggccg gcaaagtcga cctcgatgcc ctcctgatcc gcaatcccag gatgaacgcc
     1441 gacctggtcc tgcgcggcgg cgaggccctc tggggcacgg tgtacgtcga tctcgccaag
     1501 aatccggtgg acctccacgc aggggggacg cgtgccggcc ccggggagta caaaaacatt
     1561 gtcctcgaag gcggcttgga agggttcggt cgcctgggga tcgaggggaa agcgcttctc
     1621 gccggggaaa cctggcgcca ccagggacac tttgttctac gcgatgcgcg gctcgggccg
     1681 atctttcgga cctttctgag ggatccgctg gtcacgtcgc accccgacct ggcggggctc
     1741 gagacggagg ggaccgccgg gatcgacctt tccttctccg gctccgggaa agcggccgcg
     1801 ttggacggaa cgctccggct gcgatcgggc gatctgcacc ggggggcgga accgcctctc
     1861 ctctccgggc tcaacatcga cctgccgatc tcctactcct tcggcgccgc gaacccgggg
     1921 gtcaccgccc cctccgacgc ggcgaagtgg ggccgtttgc gcctggagaa gattcgcctc
     1981 tccggacagg aactggggcc tttggatatg ccggccgtgc tggtgcccaa tcatctctac
     2041 ctcgggggaa ccgtcgaggc gtccctgttc ggagccaaac ttgcactgcg gcgaatccag
     2101 gtggacgaac cgctttcccc gggctttcga atccggatgg cggcgaaact ggacgatctg
     2161 aacctttcga ggatcgccgg aaagaatccg atgctcgagg ggcgcctcgg aggcatcctc
     2221 gatcccgtga ccatcggcca ggaacggatg gacgctgacg gagagctgac cggcgatctg
     2281 ttcggcggcc ggatggacgt ccgccgcatg atggtggagc ggcccttcag cgcggggcgg
     2341 gagatcggcg ccgacgtgaa cgtcgaccgg atcgacctcg aacggttttc cgcagccctg
     2401 ggcatcggac gcatcaccgg ccgcctctcc ggatcgatta cggatttgcg ggtggcctac
     2461 ggccagccgg tggcgttcca tatcaagatg gagtcggtac ccgccaaagg ggtgagccag
     2521 tcggtaagcc tcaaggcggt gaactccatc tcgttggtga gcacgggctc cgcgttgagc
     2581 ggcatgggga tctcactgat gacgacgttt ttccgggaat ttccttacga gaagatcggt
     2641 ttcgaatccg gactgaaaaa cgacgtcttc accgttcggg gtctcatcca cgaagacggt
     2701 gtagagtatc tggtgaagag gcgctttttt gccgggatcg acgtgatcaa ccgcaacacg
     2761 gacaaccaca tcggcttttc ggatatgctg gagcgagcaa aacgcgtgac cggcgaacgc
     2821 tccaattgat gtggatgcac ccatccgacc gtgaaaaggg ggatagcatg gaacgacccg
     2881 cgcatcgcat tgctttttcc gtcctggcgg ttcttgcgct catcgcaggt tgcgtcacgg
     2941 taaacgtcta cttccctgct gcgcaggtgg aaaagacggc acagaagatc gtggacgagg
     3001 tctaccagga aaagaaagaa cctccagcgc aggagccgac ggaaaaaccg cgatcgtcga
     3061 atgatggaga cacgatccgc ggcatcgtgc gacttgcccg cttcgggccc gccccggcct
     3121 tcgccgagga agccacgacc gtaagcaacg cggcgattcg cgggctgaag gagcagatcg
     3181 gccagcggca ccaggagttg ctccccttct atcagcaagg ccaggtgggg atcacccggg
     3241 acggattcct ggaggtccgg gggacgagcg gcctcgggtt gccacaggtg gcggtcctga
     3301 agagactcgt cgacgccgac aacgccgcgc gtcgccaact ctacgaggag gtggcgaaag
     3361 ccctcaacct gaaaccggag caggtgtccc aggtccggaa gatctttgcg aagcagtgga
     3421 gggacaaggc ccagtccggc tggacggtcc agtcggacga cggccagtgg ggacgcaagt
     3481 aacggctcac ggcgggacgg tccggcgccc ccggaaacgc tactgtagtt tggggaggct
     3541 tc
//
LOCUS       DASVJL010000087         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_482206, whole genome shotgun sequence.
ACCESSION   DASVJL010000087 DASVJL010000000
VERSION     DASVJL010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482206"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VF357_01635"
     CDS             complement(<1..585)
                     /locus_tag="VF357_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522875.1"
                     /translation="MTDLAHWLVENTASLYSVNNLTGCRNKTDRRNVTLYASPWEAAL
                     SALEWGKITARLSAHASSEPGRDLCAALFPGTDLDAIRASLEENRDGRRMLSQDGALP
                     LDEAKEIREEVGKAAKGASLSPTELLRIGKTARIGERVRRFFEDRRGRYPRLAHHANG
                     IPPLRDLAEAVEQAIDANGNVLDRASEELGPLRRQ"
     gene            complement(824..1858)
                     /locus_tag="VF357_01640"
     CDS             complement(824..1858)
                     /locus_tag="VF357_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7522876.1"
                     /translation="MGILLPFGLLFLFISGCALSHKEVMAKDQMERAKNAYAEAKANP
                     NVEAYAPLQLQEAGKALQAAEQAKELDDILQLGYLAEKKARVAVTTADGKVSERDIDK
                     LNVENAELIAKKRTLESERAQRETGRAMSETERARRETGRAMSEADRARREAGAATSD
                     AEQARMAAAADAERAAIANRQAEQAKSATEVEAAKAAQAKAEADQLIKELSDLKAQQT
                     ERGIVLTIGDVLFATGKANLSQGANRSVGKLADFLKKNPNRNVLIEGHTDSVGKDDYN
                     LALSRRRADSVKDNLVGDGIEASRITTVGYGKRFPLASNDTNAGKTQNRRVEVIILNE
                     GVKADTQIRR"
     gene            complement(1920..2315)
                     /locus_tag="VF357_01645"
     CDS             complement(1920..2315)
                     /locus_tag="VF357_01645"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEX7522877.1"
                     /translation="MDGKKRRIQQVFIVVTVIGLLFAVGCAAPKTTAQLSGEKIFRAE
                     KAFSDAKEGNASLNAKDALAVAERKLANAKDAFAKQNFDEAANLAEQAAVDAEYARAK
                     ATTQKSLNIAEEIKKNNEAQRQEIQQMPK"
     gene            2547..>2795
                     /locus_tag="VF357_01650"
     CDS             2547..>2795
                     /locus_tag="VF357_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX7522878.1"
                     /translation="MRKPLPLRKGDVVAVFAPAGPVDAGRLARGIARLSAAGFVPEIA
                     DGVLAAEGYLAGGDAHRARQVEWALTLPEARAVMAARGG"
BASE COUNT          431 a          910 c          808 g          646 t
ORIGIN      
        1 ctggcgccgc aagggcccca gttcctccga cgcccggtcg agcacgttcc cgttggcgtc
       61 gatcgcctgc tcgaccgcct cggcgaggtc gcgcagcggc gggatcccgt tcgcgtggtg
      121 ggccagccgc gggtaccttc cacgccggtc ctcgaaaaac cggcggaccc gctccccgat
      181 gcgcgccgtc ttcccgatcc tcagcagttc cgtcggggag agggaggcac ccttggccgc
      241 cttccccacc tcctcgcgga tctccttcgc ctcgtccagc ggaagagcgc catcttgcga
      301 aagcatccgc cgcccgtcgc ggttctcctc gagcgacgcg cggatggcgt cgagatcggt
      361 gccggggaag agcgccgcgc acaggtcgcg ccccggctcc gaggaggcgt gggccgacag
      421 ccgggccgtg atcttccccc attcaagggc cgacaaagcc gcttcccaag gcgatgcgta
      481 caacgtcacg ttcctccgat ccgtcttgtt tcggcatccc gtgaggttgt tgacggagta
      541 aagcgaggcg gtgttctcca ccagccaatg cgcaagatcg gtcacagcct tcgggtggga
      601 aacatcgtgg cgctcgacca gggcccggaa caggatggcg tggaaatatt cctgatgaat
      661 cctgatgcgc cggatgccga gagataattg taaacgatat cgggggaaga aggttactca
      721 cggactcttc ggaacatcca gtacagaggg aaaagggcag ggaaacctcc ccgccctttt
      781 cccttggagc ctcaccccga agatgcgatc ttcaacggga tggttacctc cggatctgtg
      841 tatcggcctt gaccccttcg ttcaggatga tcacttcgac gcgacggttc tgcgtcttac
      901 cggcgttggt gtcgttgctg gccaggggga atctctttcc ataccccacg gtggtgatcc
      961 gacttgcctc gatcccgtcc cccaccaggt tgtcttttac cgaatccgct ctcctccggg
     1021 aaagggccag gttgtagtca tccttcccga cgctgtccgt atgcccctcg atcagcacgt
     1081 tccggttcgg attcttcttt aaaaaatccg ccaacttgcc cacgctcctg ttcgcgccct
     1141 gtgagagatt cgccttgccg gtggcaaaaa gcacgtcccc gatcgtcagg acgatccccc
     1201 gttccgtctg ttgggctttg agatccgaaa gctcttttat gagctggtcg gcttcggcct
     1261 tggcctgggc tgccttcgcc gcttccacct cggtggccga ttttgcctgc tctgcctgcc
     1321 gattcgcaat agcggccctc tccgcatccg cagcagccgc catccgggcc tgctccgcat
     1381 cgctcgttgc cgctcccgct tcccgcctgg cgcggtccgc ctcgctcatt gcccttccgg
     1441 tttcccgccg agcgcgctcc gtctcgctca ttgcccttcc cgtttcccgc tgggcgcgct
     1501 ccgactccag ggtccgtttc tttgcgatca gctcggcatt ttccacgttg agtttatcga
     1561 tgtccctctc cgacaccttg ccatccgcag tggtcacggc aacccgggcc tttttctccg
     1621 cgaggtagcc cagctggaga atgtcgtcca attccttcgc ctgctccgct gcctggaggg
     1681 ccttgcccgc ttcctggagc tggagcggcg cataggcttc cacgttcggg ttggccttgg
     1741 cctccgcata ggcatttttg gcgcgctcca tctggtcctt ggccatcacc tccttgtggg
     1801 agagggcgca accggaaatg aagaggaaaa gcaggccgaa cggaaggagg atccccattc
     1861 cgctttttat ccgatgccat gtcgtatgtt tcatattccg atccctccct gaagagatgt
     1921 tatttgggca tctgctggat ttcctggcgc tgggcttcgt tgtttttctt tatctcttcc
     1981 gcgatattca ggctcttctg ggtcgtggct ttggcccggg cgtactccgc gtcgaccgcc
     2041 gcctgctccg ccaggtttgc agcctcatcg aaattctgct tcgcgaaggc gtccttggcg
     2101 ttcgctaatt tccgttccgc aaccgcaagt gcatccttcg cgttcaggga ggcattgccc
     2161 tccttggcat ccgagaaggc tttttccgct cggaagatct tctcgccgga caattgtgcg
     2221 gtcgttttcg gagcagcgca tccgaccgcg aaaagcagcc cgattaccgt cacgacaatg
     2281 aacacctgct ggattctcct tttcttcccg tccatttggt gctcctttct gttctgtcgg
     2341 ttggaagatt tatttaactg aagaatttcc ggattatgtc aaaacctgca ataataatga
     2401 tcggttaaag ttttacatgt caaggataat tgccgggttc cgttctccgg tctctcgaat
     2461 gtcgcgacga tgcatcgctt tcaggccgat cggcgagttc aactgccgct cttgggatca
     2521 tacgatcatg atagcatgat cgaacgatgc gtaagccgtt gccgctgcga aaaggggacg
     2581 tggtcgccgt cttcgccccc gcggggcccg tcgatgcggg acgccttgcc cgggggatcg
     2641 cccgcctgtc cgcggcgggg ttcgttcccg agatcgccga cggtgtgctc gcggcggagg
     2701 ggtacctcgc cgggggcgac gcgcaccggg cgcgccaggt cgaatgggcg ttgaccctcc
     2761 cagaggcccg ggcggtgatg gcggcgcgcg gcggc
//
LOCUS       DASVJL010000088         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_951515, whole genome shotgun sequence.
ACCESSION   DASVJL010000088 DASVJL010000000
VERSION     DASVJL010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951515"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VF357_01655"
     CDS             complement(<1..1302)
                     /locus_tag="VF357_01655"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX7522879.1"
                     /translation="MTEGKSKRSHWRRGPRKKKVAEDAAGTAASTPPVTEGGDPGSES
                     EATWSPDTGESFAPPAPGFAEAGFDAAPADVPAAPVESPPVEMEAGSALAGEVPAKGR
                     RRPRRRGKKKPGDAPAPESLEGAGEAQPAAEEPRPFGVVGWGGPASPARESFEAPATV
                     EAAPAEGDLPAAKAEGPPGTSAEPGGEPGEPGKRRRRRRRRRRGKGKGPAQGEAGGTT
                     PTEAGDEEPAADDEGALVAGGTEAEEPDAGLEPMNETDEEEEESFPEEGTGAKKVMLI
                     DGLHSEENRVAIVAEGNLDYYEVENQRRKAFKGNIYKAKVVNIAHAIEAAFVEFGGGR
                     HGFMPLNEYCAGALMEHLGSWNEGDKRPPLRPGMEILVQVTKEESTIKGAAVTSYISI
                     AGRYMVMMLGMKRYGISKKITGEKERERIRKQMEKLEYPDHL"
     gene            complement(1299..>1996)
                     /locus_tag="VF357_01660"
     CDS             complement(1299..>1996)
                     /locus_tag="VF357_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEX7522880.1"
                     /translation="IFDLDNTLYPPEVALWRIVDARIEEYVRRKLGTDPETAHRMRKA
                     FLAECGTTLRGLMHHHGVSPGDYLEFVHDVPIPEIVPPRPELREMLSGLPGRCVVFTN
                     GSDNYARRVLDALGVAEMMEGIYGIEFMEYIAKPSPYPYAKLLRVTGARGEDSLFCED
                     SRKNLLPARELGMFTVWVGGNEKESPAHAVVKDVCDLPDVLQGFPPMSRGNGGAARGA
                     PGNGVSRKAGGTE"
BASE COUNT          235 a          782 c          599 g          380 t
ORIGIN      
        1 gaggtggtcg gggtattcga gcttctccat ctgcttccgg atccgctcgc gctccttctc
       61 ccccgtgatc ttcttcgaga tgccgtaccg cttcatcccg agcatcatca ccatgtagcg
      121 gccggcgatg ctgatgtacg acgtgaccgc ggcccccttg atcgtggact cctccttcgt
      181 cacctggacg aggatctcca tgccggggcg cagcggaggc cgcttgtctc cctcgttcca
      241 cgacccgagg tgctccatca gggcgccggc gcaatattcg ttcagcggca tgaagccatg
      301 gcggccgccc ccgaactcga cgaacgccgc ctcgatggcg tgggcgatgt tgacgacctt
      361 cgccttgtag atgttcccct tgaacgcctt ccggcgctgg ttctcgacct cgtagtagtc
      421 gaggttccct tccgcgacga tcgccacccg gttctcctcg gagtgaaggc cgtcgatcag
      481 catcaccttc ttcgcgccgg tcccctcctc gggaaaggac tcttcctctt cctcgtccgt
      541 ctcgttcatc ggctccaggc cggcgtccgg ctcctcggcc tccgtaccgc ccgcgaccag
      601 ggctccctcg tcgtccgccg ccggttcctc gtcaccggcc tccgtcgggg tcgtgccgcc
      661 ggcctcgccc tgcgccggtc ctttcccctt cccccggcgg cgacggcgcc tgcggcgacg
      721 cctcttcccg ggttctcccg gctctccacc cggttccgcc gaggtcccgg gagggccctc
      781 cgccttcgct gccggcagat cgccttccgc cggagccgcc tcgacggtcg ccggggcctc
      841 gaacgattcc cgggcgggcg acgccggtcc accccacccc acgaccccga aaggtcgggg
      901 ctcctccgcc gcgggctgcg cctcgccggc cccttccagc gactccggtg caggcgcgtc
      961 tcccggcttc ttctttcccc gccgccgcgg ccttcgccgt cctttcgccg ggacctctcc
     1021 ggccagcgcg ctccccgcct ccatttccac gggggggctt tccaccggcg cggccgggac
     1081 atcggccggc gccgcgtcga atcccgcctc cgcgaatccc ggtgcgggcg gcgcgaacga
     1141 ttcccccgta tccggcgacc aggtcgcctc gctttccgac cccggatccc ccccttccgt
     1201 gaccggcggg gtcgacgcgg cggttcccgc cgcgtcctcc gcaaccttct tcttccgggg
     1261 tccgcgtcgc caatggcttc gtttcgattt cccttccgtc attccgttcc tcccgctttc
     1321 cgggacaccc cgtttccggg cgcccctcgg gccgcgccgc cgttcccccg ggacatgggg
     1381 gggaaccctt ggagcacgtc cggcaagtcg cacacgtctt tcacgacggc gtgcgccgga
     1441 gactcctttt cgttcccgcc cacccacacg gtgaacatcc ccagttcgcg cgcgggcagc
     1501 aggttcttgc ggctgtcctc gcagaacagg gaatcctcac cgcgggctcc cgtcacccgc
     1561 agcagcttcg cgtacgggta gggcgacggt ttggcgatgt attccatgaa ctcgatcccg
     1621 tagatccctt ccatcatttc ggccacgccc agcgcatcga gcacccggcg ggcgtagtta
     1681 tccgatccgt tggtgaacac gacgcacctg ccggggaggc ccgagagcat ctcccgcagc
     1741 tccggccgcg gggggacgat ctccggaatc ggcacgtcgt ggacgaactc gaggtagtcg
     1801 ccgggggaga cgccgtggtg gtgcatcagc ccccgaagcg tcgtcccgca ttcggcgaga
     1861 aacgccttcc gcatccggtg ggccgtctcg gggtcggtcc cgagcttcct gcggacgtac
     1921 tcctcgatcc gcgcgtcgac gatccgccac agggccactt ccggcgggta gagcgtgttg
     1981 tcgagatcga agatga
//
LOCUS       DASVJL010000089         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_388427, whole genome shotgun sequence.
ACCESSION   DASVJL010000089 DASVJL010000000
VERSION     DASVJL010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388427"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VF357_01665"
     CDS             <1..314
                     /locus_tag="VF357_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7522881.1"
                     /translation="GEPDASGRRRPVPMKGSEFKLPVDTVIVAIGNGANPLVPSSTPG
                     LDTNKWGNILADQETGKTSKKGVFAGGDIVIGAATVILAMGAGRKAAAAMHEYLKTRA
                     W"
     gene            complement(327..1280)
                     /locus_tag="VF357_01670"
     CDS             complement(327..1280)
                     /locus_tag="VF357_01670"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HEX7522882.1"
                     /translation="MPVEILFAAGRVPVDLNNVFIASDLPADFIRGAEADGFPRNSCG
                     WIKGIYGVARRNDFREIVAVTQGDCSFTQALMEVLRYRGVSVVPFAYPFDRDPDFLSR
                     ELAKMAVRFRTTVAEGERWKERLAGVRRLAHEIDRLTWEEGKVTGEENHRWLVACSDF
                     DGDPDDYARRAAAFLVEASARPARNDLVPIAFVGVPPIVSGLHVCFEEAGARAVLNEV
                     QRQFAMPGITGSLVEQYLAYTYPYSFFERLEDIKAEAARREVRGIVHYVQSFCFRQIE
                     DIILREEVGVPVLTLEGDAPGPVDGRTRIRVQAFVEMLQGR"
     gene            complement(1312..1596)
                     /locus_tag="VF357_01675"
     CDS             complement(1312..1596)
                     /locus_tag="VF357_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522883.1"
                     /translation="MDQIIDFLTSKTLAEMASDPRVLFGTVVLFVLAVLFRWKYVLLV
                     LFAIGATMTVLRYTSGGGSSELVVDRSMMLFIGGTIVIALVLIYFLFIRD"
     gene            1680..2474
                     /gene="murI"
                     /locus_tag="VF357_01680"
     CDS             1680..2474
                     /gene="murI"
                     /locus_tag="VF357_01680"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX7522884.1"
                     /translation="MENPVGVFDSGVGGLTVLRELVSALPSERFVYLGDTARVPYGGK
                     SAETVTRYAIEIANHLIRTRDIKLLVVACNTASSLALPVLRKIYKIPVVGMVDPCVRR
                     AAALPEKRTIGVIGTLGTVRSGAYEEALRLSVPSARVRSIPCPLLVSLAEEGWTDNAI
                     TRAVIAEYLAPFLEDPPDALILGCTHYPVLKGPIREYLGGDPVLIDSAEEAARVVDIL
                     LSETQVRRGVAPGAVEFLVTDDPERFARVGKGFFGQELPDVGRVAL"
     gene            2521..>2764
                     /locus_tag="VF357_01685"
     CDS             2521..>2764
                     /locus_tag="VF357_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522885.1"
                     /translation="MGGPQMRMLSGFNHNIRFRGKVYHVQTEDGGKDNPQIITHAFQG
                     GAILDSVRTSYADLLERPNWQSDLKDRMKAQHLEEIR"
BASE COUNT          499 a          897 c          944 g          424 t
ORIGIN      
        1 tgggagagcc cgacgcctcg gggcggcgcc gcccggtacc gatgaagggc tcggagttca
       61 agctaccggt ggacacggtc atcgtcgcca tcgggaacgg cgccaatccg ctggtcccgt
      121 cgtcgacccc agggctcgac acgaacaagt gggggaacat cctcgcggac caggagacgg
      181 ggaagacgag caagaagggg gtcttcgccg ggggggacat cgtcatcggc gcggccaccg
      241 tcatcctcgc gatgggagcc ggccgcaagg ccgccgccgc catgcacgag tatctgaaaa
      301 cgcgagcttg gtaaggcggg gcgcgctcag cgtccttgca gcatttcgac gaacgcctgg
      361 acccggatcc tcgtccggcc gtccaccggg ccgggagcgt cgccctccag cgtgaggacc
      421 ggcaccccga cctcttcccg taagattatg tcctcgatct gccggaagca gaacgactgg
      481 acgtaatgga cgatcccccg cacctcccgc cgcgccgcct ccgccttgat gtcctccagc
      541 cgctcgaaga aggagtaggg ataggtgtag gcaagatact gctcgacgag cgagcccgtg
      601 atccccggca tcgcaaactg ccgctgcacc tcgttcagca cggcccgggc acccgcctcc
      661 tcgaagcaga cgtgcaggcc ggagacgatc ggggggacgc cgacgaaggc gatcggcacg
      721 aggtcgttcc gcgcgggccg cgccgacgcc tcgacgagga acgcggcggc ccggcgcgcg
      781 taatcgtccg ggtcgccgtc gaagtcggag caggcgacca gccaccggtg gttctcctcc
      841 ccggtgacct ttccctcttc ccacgtcagg cggtcgatct cgtgggcgag gcgtcggacg
      901 ccggcgagac gctccttcca ccgttccccc tcggcgaccg tcgtcctgaa gcggacggcc
      961 atcttcgcca gttcccggga gaggaagtcg gggtcgcggt cgaacgggta ggcgaacggc
     1021 accacggaca caccccggta ccgcagcacc tccatcagcg cctgggtgaa gctgcaatcc
     1081 ccctgcgtga ctgcgacgat ttcgcggaaa tcgttcctgc gggccacgcc gtagatcccc
     1141 ttgatccagc cgcagctgtt tcgcgggaac ccgtccgcct cggcgccccg gataaaatcc
     1201 gcggggaggt ccgacgcgat gaacacgttg ttcaggtcga cggggacgcg ccctgccgcg
     1261 aagaggatct cgacgggaat ggtggtggtg aagccgacgc ggtgcgtggg atcagtcccg
     1321 gatgaagagg aagtagatga ggacgagggc gatcacgatc gtcccgccaa tgaacagcat
     1381 catgctccgg tcgacgacca actcggacga gccccccccg ctggtgtacc gaaggacggt
     1441 catcgtcgcg ccgatcgcga acaggacgag gagcacgtat ttccagcgga acaggaccgc
     1501 caggacgaag aggaccacgg tgccgaaaag cacgcgggga tcggaggcca tttccgcaag
     1561 agtcttgctg gtgagaaaat cgattatctg atccacggag ccccctttcg gacgacgctc
     1621 ggttgacatt gatacgatag cgttttacct taaaaaaatc aaaggcttca ggagcaggct
     1681 tggaaaaccc ggtgggcgtg ttcgattccg gcgtaggtgg gttgaccgtc ctgcgggagc
     1741 ttgtgtccgc gcttccgtcg gagcggttcg tgtacctggg tgacacggcc cgcgtgcctt
     1801 acggggggaa gtccgcggaa accgtcacgc ggtacgcgat cgagatcgcg aaccacctga
     1861 tccggacccg cgacatcaaa ctcctcgtgg tggcgtgcaa caccgcctcg tccctcgccc
     1921 ttcccgtcct tcggaagatc tacaagatcc cggtggtggg gatggtggac ccgtgcgtgc
     1981 gacgtgccgc cgccctgccc gaaaagcgga cgatcggcgt catcgggacg ctcgggacgg
     2041 ttcgttccgg ggcgtacgaa gaggcgctgc ggctctccgt tccctcggct cgggtccggt
     2101 ccatcccgtg tccgctgctc gtttcgctgg cggaggaggg gtggaccgac aacgcgatca
     2161 cgcgcgccgt gatcgcggaa tacctcgcgc ctttcctgga agacccgccc gacgcgctga
     2221 tcctcgggtg cacgcattac cccgtgctga aggggccgat ccgggaatac cttggaggcg
     2281 atcccgtcct gatcgactcc gccgaggagg ccgcgcgggt cgtcgacatc ctgctctccg
     2341 agacgcaggt ccgccggggc gtggccccgg gggcggtcga gttcctcgtg acggacgacc
     2401 cggagcggtt cgcgcgggtg ggaaagggat ttttcggcca ggagctgccc gacgtgggcc
     2461 gcgtggcctt gtagcgggtt ccggcgcggc acgaaccgga aggggagagg cgaaggggcg
     2521 atgggcggac cgcagatgag gatgctctcg ggattcaacc acaacatccg gttccgggga
     2581 aaggtctacc acgtccagac ggaggacggg gggaaggaca acccgcagat catcacccac
     2641 gcgttccagg gcggggcgat cctcgacagc gtacgcacgt cctacgcgga cctgctcgag
     2701 cggccgaact ggcagtccga cctcaaggac cggatgaagg cgcagcacct cgaggagatc
     2761 cggc
//
LOCUS       DASVJL010000090         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_764508, whole genome shotgun sequence.
ACCESSION   DASVJL010000090 DASVJL010000000
VERSION     DASVJL010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764508"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VF357_01690"
     CDS             complement(<1..294)
                     /locus_tag="VF357_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX7522886.1"
                     /translation="MPALMGARLPVFATILALLTISVGAVAAEVEKLPPFPVVHGGER
                     TRFAEPGMIAPDFTVFDVEGHPFRLSEEVSRKPVLLLFWSVFCDPCRAEMATLQ"
     gene            complement(330..1424)
                     /locus_tag="VF357_01695"
     CDS             complement(330..1424)
                     /locus_tag="VF357_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522887.1"
                     /translation="MNRLQRKFPMFLLAAGIAGGMFVAGCASKEVRQEAVATVNGDEI
                     KVSELREVLGVPAGVFAVVDIPVERKKEALDQLVAVRLLEQDGRSRGIDNTSEYKEVL
                     QRNDQLVRIKALLRKEIEGKLKVTNEEIKAEIVKVKEANKGISDADAAMRAAKSVSGS
                     RIQKIQEDLIAAAKKETAAAVDPKAVARIGKEENVPDNVVLASVGDEKILYGDVKKIV
                     RGLSPGGGGPHGQPDLSKNPVLVGNVLERDLTMRALAAYAKKQGVDGSEGYKAMRQDM
                     ERFILRSLVADNVATKNAEVTDKEIEAAYAEHSAEMVRDGKKIPLAMVKEQIRAALGN
                     EKGKKVIDVYITELRKKAKITVNDAVLPKV"
     gene            complement(1485..1841)
                     /locus_tag="VF357_01700"
     CDS             complement(1485..1841)
                     /locus_tag="VF357_01700"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HEX7522888.1"
                     /translation="MIVPKRNPEVVWRLEKGLHEIAWEKARKEEEYEDLGVLTLMVKG
                     EIHQLNLVGAEIWTRRNGISALGKISAEVAALFSWEGEEAEEAVLEFLRGIEEKGWVR
                     LVERPDPSSAPGRRAD"
     gene            complement(1854..>2397)
                     /locus_tag="VF357_01705"
     CDS             complement(1854..>2397)
                     /locus_tag="VF357_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX7522889.1"
                     /translation="DAVERFARKNRVEDTLRIYHVEDRKFVRTSIPNALSIKEWDLYR
                     DALEADKIINLPIAKHHSLAGITLGLKNMMGIMGGNRGQIHYRLSECLVDIHRRVPVA
                     LTVIDAGRVLVRNGPTGGNLSDVKVFGKAFASRDVVAADVVAAEKVFRLAPRDVGHIG
                     KAMEAGLGVSSVAQIRLVEG"
BASE COUNT          390 a          870 c          648 g          489 t
ORIGIN      
        1 ctggagggtc gccatttcgg cgcggcacgg atcgcagaaa accgaccaga agagaagaag
       61 gaccggtttc cttgaaacct cctccgagag gcgaaacgga tgcccctcga catcgaagac
      121 cgtaaaatcg ggggcgatca ttcccggctc cgcgaaccgg gttctttccc ccccgtggac
      181 caccgggaag ggcgggagct tctccacctc cgcggccacg gcgccgacag agatcgtaag
      241 gagggcaaga atcgtcgcga acaccggaag gcgtgccccc atcagggcgg gcacggcgct
      301 ccttccctcc cgaccggcgg atcggcagct caaaccttgg ggagaacggc gtcgttcacc
      361 gtgatcttcg ccttcttcct caactcagta atgtacacgt cgatcacctt cttccccttt
      421 tcgttcccca acgccgcccg gatctgctcc ttgaccatgg ccaggggaat tttcttcccg
      481 tcccgcacca tctccgcgga gtgttccgcg taggccgcct cgatctcctt gtccgtgacc
      541 tcggcgtttt tcgtggcgac attgtcggcc accagactcc ggaggatgaa ccgctccata
      601 tcctggcgca tcgccttata tccctcggag ccgtcgaccc cctgcttctt tgcatatgcc
      661 gcaagggccc gcatcgtcag atcccgctcc aggacgtttc cgaccagcac cgggtttttc
      721 gaaaggtcgg gctgcccgtg cggccccccc ccccctggcg acagaccacg gacgatcttc
      781 ttgacgtcac cgtagaggat cttctcatcg ccgaccgagg cgagtacgac gttgtcgggc
      841 acgttctctt ccttcccgat ccgggccacc gccttcgggt ccaccgcggc ggccgtttcc
      901 ttcttcgcgg cggcgatcag gtcttcctgg atcttctgga tccggctccc ggagaccgac
      961 ttcgccgcgc gcatcgcggc gtccgcgtcg gagatcccct tgttcgcctc cttgaccttg
     1021 acgatttccg ccttgatctc ctcgttcgtc accttgagct tcccctcgat ctccttgcgc
     1081 agcaacgcct tgatccggac cagctggtcg ttccgttgga ggacctcctt gtactccgac
     1141 gtgttgtcga ttccgcgaga gcggccatcc tgttccagga gcctcaccgc caccagctgg
     1201 tcaagggctt ccttcttcct ctccaccggg atgtccacca ccgcgaacac gccggccgga
     1261 acgccgagaa cctcacggag ttcgctgacc ttgatctcgt cgccgttcac cgtggcgacc
     1321 gcctcctgcc gtacttcctt gctcgcgcac cccgcgacga acatcccccc ggcgattccc
     1381 gccgcgagga gaaacatcgg gaatttcctt tgcagtcgat tcacttcggt tctcccttcg
     1441 atcatctgaa aatttaaact tataccataa cccggagctg gagttcaatc ggcgcgacgg
     1501 ccgggagccg acgaaggatc cggcctctcc acgagcctca cccacccctt ctcctcgatc
     1561 ccccggagga actcgagcac cgcctcttcc gcctcttccc cttcccacga gaagagcgcc
     1621 gcgacctctg cggagatttt cccgagcgcg ctgatcccgt tccggcgggt ccagatctcg
     1681 gcacccacga ggttcaactg gtggatctct cccttgacca tcagggtgag cacgccaaga
     1741 tcctcgtact cctcctcctt gcgggccttc tcccacgcga tctcatgcag ccccttttcg
     1801 aggcgccaga ccacttcggg gttccgtttc ggcacgatca tcccgaccct ccctcatccc
     1861 tcgacgaggc ggatctgcgc gacggaagaa acccccaggc ccgcctccat cgctttcccg
     1921 atgtgtccga cgtcccgggg cgccaggcgg aagacctttt cggccgcgac tacgtccgcc
     1981 gcgaccacgt cacgggaggc gaatgccttg ccgaacacct taacgtccga gagattcccc
     2041 ccggtggggc cgttccgaac gaggaccctc cccgcgtcga tcacggtgag ggcgaccggg
     2101 acccggcggt ggatgtccac gaggcactcc gacagccggt agtggatctg gccgcggttt
     2161 ccgcccatga ttcccatcat gttcttgagc ccgagcgtga tgccggccag cgagtgatgc
     2221 tttgcgatcg gcaggttgat gatcttgtcc gcttcgagcg cgtcccggta aagatcccac
     2281 tccttgatcg acagcgcgtt cgggatggac gtccggacga acttccggtc ctcgacgtgg
     2341 tagatccgaa gcgtatcctc gacgcgattc ttccgcgcga atcgctcgac ggcgtcc
//
LOCUS       DASVJL010000091         4374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_388773, whole genome shotgun sequence.
ACCESSION   DASVJL010000091 DASVJL010000000
VERSION     DASVJL010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388773"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VF357_01710"
     CDS             complement(<1..843)
                     /locus_tag="VF357_01710"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7522890.1"
                     /translation="MKHRLRILFAAVALLLSLVPSPAVAGSAKVRQGDKQVAARQYTK
                     ALKSFRDAIRKDPKDAEAMTRIGDLHASGQGVPRSDNEAVRWYAKAASLGDAAARFAL
                     AARYASGHGVGKDPAAAAKWCRLAADQGHPGAQSLLGFLYEQGMGVPRDEAESLAWYR
                     KAAEGGDVAAQKSLAWRHLEGVGVPKDLAAAAAWYRKAAEAGDPDAQVRLAGMYRDGI
                     GVPMEPVEAATWYRKAAEKGNAEAFAGYGRALLYGRGVAQNESAAADWIRKAAERGVP
                     EGIFL"
     gene            complement(840..1937)
                     /locus_tag="VF357_01715"
     CDS             complement(840..1937)
                     /locus_tag="VF357_01715"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX7522891.1"
                     /translation="MGSERQVRRLAETLVRHSVKAKKGEIVRISTGELGRPLALEVYR
                     EVLRAGAHPLLSVGFEEANAIFYEEASAEQIAYLPPTKMNEARTIDADIIILAPGNTR
                     NLSHIPPRKMADRRKAIKPISEVLLRRVRWVLTNFPTEALAQETDRSLPEYEKLYYRA
                     VEQDWAAMSRMFTRAKRVLEKADRVRIVGKETDLSFSIKGRTAIPCAGEFNLPDGEIF
                     TAPIETTTEGKIFFEFPAIAGGREVAGIRLTFRKGRVGESSAEKNEGYLKEMLAADRG
                     ASVLGEFGIGSNAGVTSFTRDILLDEKMGGTIHLAVGRSYPESGGRNDSAVHWDMIKD
                     LRAQGDLYLDGKPVLRTGVLFGKTPKGMRKA"
     gene            complement(1959..2549)
                     /locus_tag="VF357_01720"
     CDS             complement(1959..2549)
                     /locus_tag="VF357_01720"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEX7522892.1"
                     /translation="MDFETIRELAKHHKQSFGRILKCEMYKLEDGRLLAITRLHDDFH
                     DMNLAILLSDSYRIEEIAGKMDRIPQPCCETKPLEMLSSLKGIAVLERGGIKKVKQRI
                     PRNMSCTHIYEMLESTFRSIFVGSYSILGQKWDGVLTLEMEENRQLGIQSPVLAGTCY
                     AFNLESADPVVLERARKKVEEAHRKMAAIEAVKRGE"
     gene            complement(2572..3432)
                     /locus_tag="VF357_01725"
     CDS             complement(2572..3432)
                     /locus_tag="VF357_01725"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX7522893.1"
                     /translation="MIRKGPPLGAHVSVAGGVHTAPERGRRIGADVVQIFSKQNTRWM
                     GKALEEEDARALRDESERTGVRVAAIHCAYLINLGSAKETVRTRSLYALEDEASRAAM
                     LDVPYLVMHPGSCGDDPVEEGVSRIASAIRSFGKFPKGVTLLLENTAGQGNSIGRTME
                     QLRELLDAAGNPADVAVCLDSAHLFESGYDIGTAAGWDALFAEMKEKGILPRVRMWHL
                     NGSRTAMGSLVDRHEHIGEGQIDVSAFRRILNHKEFSTLPMVLETPKGEDDEVTMDLR
                     NLAILRDLIA"
     gene            complement(3429..3725)
                     /locus_tag="VF357_01730"
     CDS             complement(3429..3725)
                     /locus_tag="VF357_01730"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX7522894.1"
                     /translation="MSGIRARDFQFLLVVAAVVGLLIVLSMSGKERFIPRTEAHLAAV
                     PIQDTAKADAMCWSCHDGAKPASAEVKKGPPMPKNHPLRKKNCRQCHRLERKKS"
     gene            complement(3730..4218)
                     /locus_tag="VF357_01735"
     CDS             complement(3730..4218)
                     /locus_tag="VF357_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEX7522895.1"
                     /translation="MSEVGEKVMQATRQRAAVYAHLFLVLRKRLGEREAVDLMSEAIY
                     NFGREKSTRNYSEKARSGDLAQAAREFASPDPVKQHQFAPRVVSLTPDEAVIAMSKCP
                     LVDEWRAMGLPDKDVETLCHVAHSVDFGTWEGALRCALCFEGTRGEGKDECVLRVKKA
                     RG"
BASE COUNT          651 a         1572 c         1336 g          815 t
ORIGIN      
        1 gaggaagatc ccttccggga cgccgcgctc cgcggccttc cggatccagt ccgccgccgc
       61 cgactcgttc tgggccacgc cgcggccgta gagaagcgcc cgtccgtacc ccgcgaacgc
      121 ctccgcgttc cccttctccg cggccttccg ataccacgtt gccgcttcga ccggttccat
      181 cgggaccccg atcccgtccc ggtacatccc ggcgagccgc acctgggcgt cgggatcgcc
      241 ggcttccgct gctttccggt accacgccgc ggccgccgcg aggtctttcg ggaccccgac
      301 cccttcgagg tgcctccatg cgagcgactt ctgcgccgcg acgtccccgc cctcggcggc
      361 cttccggtac cacgcgaggg attccgcctc gtcccgcggg acgcccatcc cctgttcgta
      421 cagaaacccg aggagggatt gcgcgcccgg gtgtccctgg tccgccgcca ggcggcacca
      481 tttcgccgcc gccgccgggt ctttcccgac gccgtgaccg ctcgcgtacc gggcggccag
      541 ggcaaagcgc gccgcggcgt ccccgaggga cgccgccttc gcgtaccacc tcacggcctc
      601 gttgtcgctg cggggaactc cctgtcccga cgcgtggaga tccccgatcc gcgtcatcgc
      661 ctccgcgtct ttcgggtctt tcctgatggc gtcacggaaa cttttcaggg ccttcgtgta
      721 ctggcgggcc gcgacctgct tatccccctg ccggaccttc gcgcttccgg cgaccgccgg
      781 ggacggaacg agggaaagaa ggagcgccac ggcggcgaag agaatccgca ggcggtgttt
      841 cacgccttgc gcatcccctt cggcgtcttc ccgaacagga cgcccgtccg caggaccggc
      901 ttcccatcga ggtagagatc gccctgcgcg cgcagatcct tgatcatgtc ccagtgaacg
      961 gcggagtcgt tcctcccccc cgattcgggg tacgaacggc cgaccgccag gtggatcgtc
     1021 ccccccatct tctcgtcgag gaggatgtcg cgggtgaagg aggtcacccc ggcgttcgac
     1081 ccgatgccga actcgcccag aacggacgcc ccgcggtcgg cggcgagcat ctccttcagg
     1141 tacccttcgt tcttctcggc cgaagactcc cccacccgcc ccttgcggaa cgtcaggcgg
     1201 atccccgcca cctcgcgccc gccggcgatc gcggggaatt cgaagaagat cttcccctcc
     1261 gtggtggttt cgatcggcgc ggtgaagatc tcgccgtcgg gcaggttgaa ttcgccggcg
     1321 caggggatcg cggtccgccc cttgatcgag aaggaaaggt cggtttcctt ccccacgatc
     1381 cgcacccggt cggccttctc gagcaccctt ttcgcccggg tgaacatccg cgacatcgcc
     1441 gcccagtcct gctccacagc ccggtaatac agtttctcgt actcgggcaa ggaccggtcc
     1501 gtctcctgcg cgagcgcctc cgtcgggaag ttcgtgagga cccaccggac gcggcgcagc
     1561 agcacctcgg agatcggctt gatcgccttg cggcggtccg ccatcttccg cgggggaatg
     1621 tgggagaggt tccgggtgtt gccgggagcg aggatgatga tgtcggcgtc aatcgtcctc
     1681 gcctcgttca tcttcgtggg cggcaagtag gcgatctgct ccgcggacgc ctcctcgtaa
     1741 aagatcgcgt tcgcctcctc gaaccccacg gagagcaggg ggtgcgcccc cgcccgcaac
     1801 acctcccggt agacctccag cgcgagggga cggccgagtt cgcccgtgga gatccggacg
     1861 atctcccctt ttttcgcctt caccgaatgg cggaccagcg tttccgccag ccgccgcacc
     1921 tgccgctcag agcccatgga tctccctttc cgtttgtttt actcgccccg cttgaccgct
     1981 tcgatcgccg ccatcttccg gtgtgcctcc tcgaccttct tccgggcgcg ttcgaggacg
     2041 acggggtcgg ccgactccag gttgaaggcg tagcaggtgc cggccagcac cggggactgg
     2101 atcccgagct ggcggttctc ctccatctcg agggtgagca ctccgtccca cttctggccg
     2161 aggatgctgt agctccccac gaagatggat cggaacgtcg actccagcat ctcgtagatg
     2221 tgggtgcagc tcatgttccg ggggatccgc tgcttcacct tcttgatccc tccccgctcc
     2281 aggacggcga ttcccttcag ggacgagagc atctcgaggg gcttcgtctc gcagcagggc
     2341 tggggaatgc gatccatctt tcccgcgatc tcctcgatgc gataggaatc ggagaggagg
     2401 atcgcaaggt tcatgtcgtg aaaatcgtcg tgaagacgcg tgatcgccaa caggcggccg
     2461 tcctcgagct tgtacatctc gcacttgagg atgcgcccga agctctgctt gtggtgcttg
     2521 gccagttccc tgatcgtttc gaagtccatc gtctcccctt ttccgccccg gctacgcgat
     2581 gaggtcgcgc agaatcgcca ggttgcgcag atccatcgtg acctcgtcgt cctcgccctt
     2641 cggcgtctcg agcaccatcg gcagcgtcga gaattccttg tggttcagga tccggcgaaa
     2701 ggcggagaca tcgatttgcc cctcgccgat gtgctcatgc cggtccacga ggcttcccat
     2761 cgccgtcctc gatccgttca ggtgccacat ccggacccga gggaggattc ctttctcctt
     2821 catctccgcg aaaagggcgt cccaccccgc cgccgtcccg atgtcgtacc cggactcgaa
     2881 caggtgggcg ctgtccaggc acaccgcaac gtccgcaggg ttcccggcgg catcgagcaa
     2941 ctcccgaagc tgctccatcg tgcggccgat cgagtttccc tgcccggccg tgttttcgag
     3001 gagcagcgtc acccccttcg ggaacttccc gaacgaacgg atcgccgatg cgatgcggga
     3061 gaccccctcc tccaccggat cgtcgccgca cgagccggga tgcatcacga ggtacggcac
     3121 gtcgagcatg gcggcgcgcg acgcctcgtc ctcgagcgcg tagagggaac gggtgcggac
     3181 cgtctccttc gcggacccga gattgatgag gtaagcgcaa tgaatagccg cgacgcggac
     3241 gcccgttcgt tcgctctcgt cccgcaacgc cctcgcgtct tcttcctcga gggccttccc
     3301 catccagcgg gtgttctgct tggagaagat ctggacgacg tcggcgccga tccttctgcc
     3361 ccgctccggc gccgtgtgga cccctcccgc gacggagacg tgcgccccca gcgggggccc
     3421 tttccggatc acgacttctt ccgctccagc cggtggcact gacggcagtt tttcttgcgc
     3481 agcgggtgat ttttcggcat cggggggcct ttcttcacct ccgcggaagc cggcttcgcc
     3541 ccgtcgtggc acgaccagca catcgcgtcg gccttcgcgg tgtcctggat cggcacggcg
     3601 gccaggtgcg cctcggtccg cgggatgaac cgctccttcc ccgacatgga aaggacgatg
     3661 agcaggccta cgaccgcggc cacgacgagc agaaactgga aatccctcgc ccggatcccg
     3721 ctcaccgcgt cagccccgcg ccttcttcac gcggaggacg cactcgtcct tcccctcccc
     3781 gcgggttccc tcgaagcaga gcgcgcaacg caacgcccct tcccacgtcc cgaagtcgac
     3841 ggagtgggcg acatggcaga gcgtttccac atccttgtcc ggcagcccca tcgctcgcca
     3901 ctcgtcgacc agggggcact tcgacatcgc gatcaccgcc tcgtcggggg tgagcgacac
     3961 gacccgcggg gcgaactggt gctgcttcac cggatccgga gacgcgaact ccctggcggc
     4021 ctgcgcaagg tcgccggagc gcgccttctc cgagtagttg cgcgtcgatt tctccctgcc
     4081 gaagttgtag atcgcctcgc tcatcaggtc gaccgcctcc cgctccccga gccgcttgcg
     4141 cagcacgagg aacaggtgcg cgtaaaccgc cgcgcgctgg cgggtcgcct gcatcacctt
     4201 ttcgccgact tccgacatgg gaccccctgg tgtccacctt tcgagcgtca acatttgatt
     4261 gtaagaccgg cgggggcgga ttttcacctg ttttatcggg gaggcgacgg aggcggatat
     4321 tccgatcggg ggaccgcagt gtgacgtctg gagcaaaatg gagacagtga tatc
//
LOCUS       DASVJL010000092         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1140034, whole genome shotgun sequence.
ACCESSION   DASVJL010000092 DASVJL010000000
VERSION     DASVJL010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140034"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VF357_01740"
     CDS             complement(<1..516)
                     /locus_tag="VF357_01740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7522896.1"
                     /translation="MNGEGGRFGNAVSAIGASKWIVAITVMLPTLIEIIDTSVANVAL
                     DHIRGSLSSGIDESAWVLTSYLVSNAIIIPMTGWLARTFGRKRYLTFSVLLFTFASLL
                     CGSSTSLGMLIFFRVLQGIGGGALQPLSQAILLETFPPKEHGMAMAIFGIGAMFGPIA
                     GPLMGGYITDTL"
     gene            complement(527..>1691)
                     /locus_tag="VF357_01745"
     CDS             complement(527..>1691)
                     /locus_tag="VF357_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX7522897.1"
                     /translation="SPAPRNGRKRKAIGLFLLVLAATVIAGFAYWRYRQTHVSTDDAY
                     IDGRIHLISARVQGNVTEVQVKDNQPVRTGDPLLRIDPEPFSVRESGAVSAVAAGAGD
                     AAAARADLVAARSDVTAAIKDLEGAKAQLAQLSAGIEAARARTSLAAARRSQASRDAA
                     RIKQLFERQVVSREAFEKAQTDAEVATAQDDVAKEELRLAEAALPTQEATIAQKGAVI
                     SQRKSVATQREARIRQFEAQVQQRESALAEAKLLLRYTSVLSPADGYVTRKSVEAGQV
                     VSPGQSLLAVAALDNVWVMANFKETDVERIRPGQVVEIRVDTYKGKTFKGTVDSIMAG
                     TGSAFALFPPENASGNYVKVVQRVPVKIVLSRGEDPGHILRIGMSVVPTILVR"
BASE COUNT          260 a          584 c          570 g          277 t
ORIGIN      
        1 gagggtgtcc gtgatgtacc cgcccatcag ggggccggcg atggggccga acatggcgcc
       61 gatgccgaag atggccatcg ccatcccgtg ctccttcggc gggaacgtct cgagcaggat
      121 cgcctgggag agcggctgga gagccccccc gccgattcct tgcaggacgc ggaagaagat
      181 gagcatcccg aggctcgtgg aggagccgca caacagggag gcgaaggtga acaggaggac
      241 ggagaaggtg agataccgct ttcgcccgaa ggtgcgggca agccaccccg tcatcgggat
      301 gatgatggcg ttggagacga ggtacgacgt gagcacccac gccgactcgt cgatcccgga
      361 cgacagggac ccgcggatgt ggtcgagggc gacgttcgcg acgctcgtgt cgatgatctc
      421 gatcagcgtc ggcagcatca ccgtgatcgc gacgatccac ttgctcgcgc cgatcgcgct
      481 gaccgcgttc ccgaaccgcc ccccctcccc gttcatcggg cgtccgtcag cggacgagga
      541 tcgtggggac gacggacatt ccgatccgca ggatgtggcc cggatcctcc ccccgcgaaa
      601 ggacgatctt caccgggacg cgttgcacga ccttcacgta gttccccgag gcgttctccg
      661 gcggaaagag ggcgaaggcg gagccggtcc ccgccatgat gctgtcgacc gttcccttga
      721 acgtctttcc cttgtatgta tccacgcgga tctccactac ctgccccggg cggatccgct
      781 cgacgtccgt ttccttgaag ttcgccatca cccacacgtt gtccagggcc gccacggcca
      841 ggagcgactg gccgggggaa acgacctggc ccgcctcgac gctttttcgg gtgacgtatc
      901 catccgccgg ggagaggacc gaggtgtagc ggaggagcag cttcgcctcc gccagggcgg
      961 actcccgctg ctgaacctgc gcctcgaact gccggatgcg ggcttcgcgc tgggtcgcca
     1021 cggatttccg ctgggagatg accgccccct tctgcgcgat ggtcgcctcc tgcgtcggaa
     1081 gagcggcctc cgcgagccgc agttcctcct tcgccacgtc gtcctgcgcc gtcgcgacct
     1141 ccgcgtcggt ctgggccttc tcgaacgcct cccggctgac cacctgccgc tcgaagagct
     1201 gctttatccg cgcggcgtcc cgggacgcct gggatctccg tgccgcggca agggaggtcc
     1261 ttgcgcgggc cgcctcgatg ccggcagaaa gttgcgcgag ctgcgccttc gccccctcca
     1321 ggtccttgat ggcggccgtt acgtccgacc gggcggcgac gaggtcggcc cgggcggccg
     1381 ccgcatcccc cgctccggcg gcgacggcgg aaacggcccc ggactcccgc acggagaacg
     1441 gctccgggtc gatccgcagg agaggatccc ccgtcctgac gggctggttg tccttcacct
     1501 gaacctccgt cacgttcccc tggacgcgcg cggagatcag gtggatcctt ccgtcgatat
     1561 aggcgtcgtc ggtgctaacg tgggtctgcc ggtaccgcca gtaggcgaac ccggcgatca
     1621 ccgtcgcggc gaggacgagg agaaacaggc cgatcgcctt ccgtttccgg ccgttccggg
     1681 gcgccgggct g
//
LOCUS       DASVJL010000093         3597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1515313, whole genome shotgun sequence.
ACCESSION   DASVJL010000093 DASVJL010000000
VERSION     DASVJL010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515313"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VF357_01750"
     CDS             complement(<1..263)
                     /locus_tag="VF357_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522898.1"
                     /translation="MGEIRARDFQFLLVVVAVIGLLTVLSMTGKERFIPRTEAHLAVA
                     PIQDTAQADAVCWSCHDGAKPASAEVKKGPPMPKNHPLRKKNCR"
     gene            complement(268..756)
                     /locus_tag="VF357_01755"
     CDS             complement(268..756)
                     /locus_tag="VF357_01755"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEX7522899.1"
                     /translation="MSEVGEKVAQATRQRAAVYAHLFLVLRKRLGEREAVDLMSEAIY
                     NYGREKSTRNYSEKARSGDLAQAAREFASPDPVKQHQFAPRVVSLTPDEAVLAMSKCP
                     LVDEWRAMGLPDKDVETLCHVAHSVDFGTWEGALRCALCFEGTRGEGKDECILRVKKA
                     RG"
     gene            complement(992..1378)
                     /locus_tag="VF357_01760"
     CDS             complement(992..1378)
                     /locus_tag="VF357_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522900.1"
                     /translation="MRVHYVLNHGGTPASKLADVEIHFEEGLLAGLKLVGCSVWRSKK
                     GDAPTVLVPSRSYATAGGVRYYELLRSSVDGAASAGGNVEDPAAKLAVRRFKEYVRDE
                     YRKIAALPDDPPVPAERSGGKKTAAR"
     gene            complement(1440..>3597)
                     /gene="priA"
                     /locus_tag="VF357_01765"
     CDS             complement(1440..>3597)
                     /gene="priA"
                     /locus_tag="VF357_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX7522901.1"
                     /translation="PFGEEHRAEVGVRVLIPFGGRKMTGLVTAITDASGLGGRDAKDL
                     LAILDDAPYVSERHLAFLTATTLGCLAPLGETLRAALPRGLPRREAPAAPRMEAFYRP
                     SPFPPEGPMTPKQRLAHEAIREAGGLSSSDLSLRVPGGAEAAKRLAVKGFLQVESRSR
                     PVTLHAASLPDLAGDLSPTPGQEAALARIGVAVASGRHAAFVLHGVTGSGKTEVYLRA
                     VEQVRATGRQAIFLVPEIALTPQLLGRVRSRFRDGVAVLHSGLTPAERSSQWRKIRDG
                     EVFLCVGARSAIFSPFPSVGLVVVDEEHDAAYKQEDGVRYQARDLALLRGRMEDAVVL
                     LGSATPSAESFHRVRTGAATLLSLPDRIGGSGMPEISVVDLKERTDRRGADRYFSTEL
                     ETEIDATLARGEKAMLFLNRRGFAAALTCLECGTTVQCRNCQVALTYHREHEALLCHY
                     CNVMRKEPESCEKCGGHKLAQVGIGTERLLSWVSKRWTEARVARLDSDVTRKRGAYAE
                     VLSGMHRGEVDILVGTQMIAKGHDFPEVTFVGVLLADLSLSFPDFRASERTFQILTQV
                     AGRSGRGDRPGKVLFQTMAPESPAIRRAAEHDYAGFMEEELAAREAMGYPPFGRMLLL
                     RLSGAKQDAAREAADLVTGALSGPMSAHGVRLLGPAPSPIARVKRRFHYQILLVMPPD
                     LPVGDLFPELLRPLRERVRKLGVRLEADVDPYQMMV"
BASE COUNT          543 a         1279 c         1221 g          554 t
ORIGIN      
        1 cggcagtttt tcttgcgcag cgggtgattt ttcggcatcg gggggccttt cttcacctcc
       61 gcggacgccg gcttcgcccc gtcgtggcac gaccagcaca ccgcgtcggc ctgcgcagtg
      121 tcctggatcg gggcgacggc caagtgcgcc tcggtccgcg ggatgaaccg ctccttcccc
      181 gtcatcgaga ggacggtgag cagaccgatg accgcgacca cgacgagcag aaactggaag
      241 tcccttgccc ggatctcgcc catcgcgtca gccccgcgcc ttcttcacgc gaaggatgca
      301 ctcgtccttc ccctccccgc gggttccctc gaagcagagc gcgcagcgca acgccccctc
      361 ccacgtcccg aagtcgacgg agtgggcgac atggcagagc gtttccacat ccttgtccgg
      421 cagccccatc gcccgccact cgtcgaccag ggggcacttc gacatcgcca ataccgcctc
      481 gtcgggggtg agcgacacaa ccctcggggc gaactggtgc tgcttcaccg gatccggaga
      541 cgcgaactcc ctggcggcct gcgcaaggtc gccggagcgc gccttctccg agtagttgcg
      601 cgtcgacttc tccctgccgt agttgtagat cgcctcgctc atcaggtcga ccgcctcccg
      661 ctccccgagc cgcttgcgca gcacgaggaa caggtgcgcg tagaccgccg cgcgctggcg
      721 ggtcgcctgc gccacctttt cgccgacttc cgacatggga ccccctggtg ttcgccgttc
      781 gagcattaac ttttaattat aagtccgggg gggcggtttt cacctgtttt atcgtagtcg
      841 ctacagggct cgcggggggc ttcctctccg ctacggctcg tcgaccctcc atggtctcct
      901 cgcctgcgct gtttctccct ctcgccatcc ctggctccgg ttcgaaacag acgccgctgc
      961 gaggaatccc ccgctgcgcc ctttgtagcg actaccgtgc ggcggttttt ttcccgccgc
     1021 tccgctcggc ggggacgggc ggatcgtcgg gcagcgccgc gatcttgcgg tactcgtcgc
     1081 ggacgtactc cttgaaccgc cggacggcga gcttcgccgc cgggtcctcg acgtttcccc
     1141 ccgcggaggc agcgccgtcg accgaggagc ggagcagctc gtagtagcgg accccaccgg
     1201 cggtggcgta ggagcgggag gggacgagaa cggtcggcgc gtcccccttc ttcgaccgcc
     1261 agacggagca ccccaccagc ttgagtccgg cgaggagccc ctcctcgaag tggatctcga
     1321 cgtcggccag cttcgacgcc ggcgtccccc cgtggttcag aacgtagtgc acccgcatgg
     1381 tcttgccctc ctttgcgcgt tgatgcgcga gaggagttca agcaccgtgc cggggaagct
     1441 cacaccatca tctggtacgg gtcgacgtcg gcctcgaggc gcacgccgag ctttcggacc
     1501 cgctcgcgga ggggacgcag gagctccggg aagagatccc ccaccggaag gtcggggggc
     1561 atcaccagca ggatctgata gtggaagcgg cgcttgaccc gggcgatcgg cgagggggcg
     1621 gggcccagga ggcgaacccc gtgggcggac atcgggccgg aaagcgcacc ggttaccagg
     1681 tcggccgcct cccgcgcggc gtcctgcttc gctcccgaca ggcgaagcag cagcatccgg
     1741 ccgaaggggg ggtaccccat cgcctcccgc gcggcgagct cttcttccat gaatccggcg
     1801 tagtcgtgct ccgccgccct gcgaatggcc gggctctccg gcgccatcgt ctggaagagg
     1861 accttgcccg gccggtcgcc gcgacccgaa cgtcccgcca cctgggtcag gatctggaag
     1921 gtgcgctccg atgcccggaa gtcggggaac gacagggaga ggtcggccag cagcaccccg
     1981 acgaaggtga cttcggggaa gtcgtgtccc ttggcgatca tctgcgtgcc gaccaggatg
     2041 tccacctccc cccggtgcat gccggagagg acctcggcat acgccccgcg cttccgtgtg
     2101 acgtccgagt cgagccgggc gacccgcgcc tccgtccacc gcttcgagac ccaggagagg
     2161 agccgctccg tcccgatccc cacctgcgcg agcttgtgcc cgccgcactt ctcgcacgat
     2221 tcgggctcct tgcgcatcac gttgcagtag tggcacagga gcgcctcgtg ctcccggtgg
     2281 tacgtgagcg ccacctggca attccggcac tgcaccgtgg ttccgcactc gaggcacgtg
     2341 agggcggcgg caaacccgcg ccggttgagg aagagcatcg ccttctcgcc ccgcgcaagg
     2401 gtcgcgtcga tctccgtctc gagctcggtg gagaagtagc gatcggcgcc gcgccggtcc
     2461 gtgcgctcct tgagatcgac cacggagatc tccggcatgc cgctcccgcc gatccggtcg
     2521 gggagggata ggagcgtcgc ggcgccggtc cgcacccggt ggaacgactc ggcggaaggg
     2581 gtggcggagc ccagcaggac gacggcgtcc tccatgcggc cccgcagaag ggcgaggtcc
     2641 cgcgcctggt accggacgcc gtcctcctgc ttgtacgcgg cgtcgtgctc ctcgtccacg
     2701 acgacgagtc ccacggacgg gaagggggag aagatggcgg agcgcgcccc cacgcagagg
     2761 aatacctccc cgtcgcggat cttcctccac tgggaggacc gctccgccgg cgtgagcccg
     2821 ctgtggagca ccgcgacgcc gtcgcggaaa cgggagcgga ccctgccgag cagctggggg
     2881 gtgagggcga tctcggggac gaggaagatc gcctggcggc ccgtggcgcg cacctgctcg
     2941 accgcgcgca ggtagacctc ggtcttcccg gaaccggtca cgccgtgcag cacgaaggcg
     3001 gcgtgccgcc ccgaggcgac ggcgaccccg atccgcgcaa gcgcggcctc ctggccgggc
     3061 gtgggagaga gatcccccgc cagatcggga agggacgcgg catggagggt caccggccgg
     3121 gaccgcgact ccacctgcag aaagcctttt acggcaagac gcttcgccgc ctccgctccc
     3181 cccggaaccc gcaacgaaag gtcggacgaa gagagcccgc cggcttcgcg gatcgcttcg
     3241 tgcgccaggc gctgcttcgg agtcatcgga ccttcggggg gaaacggtga agggcggtag
     3301 aacgcctcca tcctgggcgc agcgggtgct tccctccggg gcagcccgcg agggagagcg
     3361 gcgcgcagcg tctcgcccag cggggcgaga cacccgaggg tcgtcgcggt gaggaacgcg
     3421 aggtgccgct ccgacacgta cggcgcatca tcgaggatcg cgaggagatc cttcgcgtcc
     3481 cgtccgccga ggccggaggc gtcggtgatc gccgtgacca accccgtcat cttccgcccg
     3541 ccgaacggga tgaggacgcg aacgccgacc tccgcccggt gctcttcccc gaagggg
//
LOCUS       DASVJL010000094         3596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_295933, whole genome shotgun sequence.
ACCESSION   DASVJL010000094 DASVJL010000000
VERSION     DASVJL010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295933"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3084
                     /locus_tag="VF357_01770"
     CDS             <1..3084
                     /locus_tag="VF357_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7522902.1"
                     /translation="ISLFVSFTLDPMLSSRWIDPDIERKGKRHLVARILDRFNGWFDR
                     TADVYRSMIAWALGHRKTMLALATLAFFGGLFAFTSLKTEFFTPVDRAEFQINFKSAP
                     DASLVETRDRVEAVLAEIRKIREVRRTFATVGAGDAGTVRDGMVYVKLSEKKERKRRQ
                     DEIQKEVRERLGNIPAIIPAILEVGRLAGEKPFNVAVRGEDIGLLKGYAAALKREIRK
                     IPGIVDLEVTLEHDIPEYRLTVDGKRAADLGVTTGTVVRTVGALIGGQVVSTYEDPDG
                     DAVNVRVRLPVAMRRDPSQVEGVRLAVNRGPEGVALVPLGEVARYSLSDTPSEINRQA
                     LTREVVLSANLDGLPLGEAMNKVKEITGKMPMAPGYHVVFTGEGEDMIETFGYMAEAL
                     VLAVIFVYLILAAQFESFIEPFSIMLSLPLSIVGMAGMLLLTGDTVNIMSLIGLIMLM
                     GLVTKNAILLVDYAKVLQTRGMERAEAVITAGRTRLRPILMTTLAMIFGMLPLALGIG
                     AGAEERAPMARAVVGGLITSTFLTLLVVPVVYTLLDDFAAWVRRRWDGKKVAAVAVVG
                     LLVLLLGVPSPGPPAAAAADSAAVEILTLDDALRAAVVSNRDVGKAMELRNSLEGKYV
                     EERAAALPQFLATAEALRSWDESQAIFGAPPGSNRYTAQVGVSQPLYSSGAVTAGIRA
                     AGKGLATADDRLRIARHAVLRDVYAAFHDILLGRELNRIAIEDQSQKARLLDEARKKY
                     AAGTATDYDVLAAEVALRNAGPEVVRTENTVRTVRERLRFLLGRDRTEVDAKGDLAVA
                     VVDPPNYDMALGTALEHRPELSDLRNRQGVAEELLVIARAGNRPRLDFRGALGWQEID
                     FGVVDLTGKTWSVGLFASWPLFDGFRTRGRVAQAGSDVRTLRIEEAQLLDAIALEVRD
                     AANAVREAGEIVNALAGTVDQADRLLAMAEKGYEYGVKTRLDVDDAQLNRSRALGNLA
                     RARRDYLVAGAALRHAMGTLGDGIVPPKGEKQAFRPAASPVGLVGEVLNGRPVLP"
     gene            complement(3171..3467)
                     /locus_tag="VF357_01775"
     CDS             complement(3171..3467)
                     /locus_tag="VF357_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522903.1"
                     /translation="MQVSKRMAIHGLNISMAVFTAFSAYGLLLYYEGGDDAAKTTTLV
                     TMLSYLAVIAACIAGIGFFGVQLDRRKSEDRREEEARKAAEAERKGPRKPLQRT"
BASE COUNT          585 a         1124 c         1299 g          588 t
ORIGIN      
        1 atctccctct tcgtgtcgtt caccctcgac ccgatgctct cttccaggtg gatcgacccc
       61 gacatcgagc gaaaggggaa acggcatctc gtcgcccgga tcctcgaccg gttcaacggc
      121 tggttcgacc ggacggccga cgtgtatcga tcgatgatcg cgtgggccct cgggcatcgc
      181 aagacgatgc ttgccctcgc gacgcttgcg ttcttcggcg gcctcttcgc gttcacgtcc
      241 ctcaagaccg agttcttcac gccggtggac cgagccgagt tccagatcaa cttcaagtcg
      301 gcgccggatg catctctcgt cgagacgcgg gaccgcgtcg aagccgtcct tgcggaaatc
      361 cgcaagatcc gcgaggtccg ccgcaccttc gcgaccgtcg gggccgggga cgcggggacg
      421 gtccgggacg gcatggtgta cgtgaagctc tccgaaaaga aggagaggaa acggcgtcag
      481 gacgagatac agaaggaggt gcgggagcgg ctggggaata tccccgcgat tatccctgcg
      541 atcctcgagg tgggcaggct cgccggggag aagccgttca acgtagccgt gcgcggggag
      601 gacatcggcc tgctcaaggg atacgcggcg gccctgaaac gcgagatccg gaagatcccg
      661 gggatcgtcg acctcgaggt gaccctggag cacgatatcc cggagtatcg cttgaccgtg
      721 gacgggaagc gggcggcgga cctgggcgtg acgaccggga ccgtcgtgcg caccgtgggc
      781 gcgctgatcg gggggcaggt ggtctcgacc tacgaggacc cggacggcga cgcggtgaac
      841 gttcgcgtcc ggctgcccgt agcgatgcgc cgggacccgt cccaggtgga gggggtccgg
      901 ctggcggtga accgggggcc ggaaggggtt gcccttgtgc cgctgggaga agtggcccgg
      961 tattcgctga gcgacacccc ttccgagatc aaccggcagg cgctgacccg ggaggtcgtc
     1021 ctctcggcga acctcgatgg gttgccgctt ggggaggcga tgaacaaggt caaggagatc
     1081 accggcaaga tgccgatggc tccaggctac cacgtcgtct tcaccgggga gggagaggac
     1141 atgatagaga cgttcggcta catggccgag gcgctggtcc tcgccgtgat cttcgtctac
     1201 ctgattctcg cggcgcaatt cgaatcgttc atcgagccgt tctccatcat gctttcgctt
     1261 cccctgtcga tcgtcgggat ggcggggatg ctgctgctga cgggggacac ggtgaacatc
     1321 atgtccctca tcggcctcat catgctgatg gggctggtga cgaagaacgc gatcctgctc
     1381 gtggattacg cgaaggtgct gcagacgcgg gggatggagc gggccgaggc ggtgatcacg
     1441 gcgggcagga cgaggctgcg gcccatcctg atgacgacgc tggcgatgat cttcgggatg
     1501 ctcccgctgg cgctggggat cggtgcgggg gcggaggagc gggcgccgat ggcgcgggcc
     1561 gtcgtcgggg ggctgataac gtcgaccttc ctcacgctgc tcgtcgtgcc ggtcgtctac
     1621 acgctgctgg acgacttcgc cgcctgggtg cgacggagat gggacgggaa gaaggtcgcg
     1681 gcggtcgccg tcgtcggcct gctggtcctg ctcctcgggg tcccttcccc cggccccccg
     1741 gcggcggccg ccgccgattc cgccgccgtg gagatcctta cgctcgacga cgcccttcgg
     1801 gcggccgtcg tgagcaaccg ggacgtcggg aaggcgatgg agctgcggaa ctccctcgag
     1861 gggaagtacg tcgaggagcg tgcggccgcc cttccgcagt ttctcgcgac tgcggaggcg
     1921 cttcgctcct gggacgaaag ccaggccatt ttcggcgcac ctcccggcag caaccggtat
     1981 accgcccagg tgggcgtttc gcagcccctt tactcttccg gggccgtgac cgccgggatc
     2041 cgggcggcag gcaaaggcct ggcgacggcg gacgaccggc tccggatcgc ccggcacgcc
     2101 gtcctgcggg acgtatacgc cgcgtttcac gacatcctgc tcgggaggga attgaaccgg
     2161 atcgcgatcg aggaccagtc gcagaaggcg cggctcctcg acgaggcgcg gaagaaatac
     2221 gcggccggga ccgccaccga ctacgacgtt ctcgcggcgg aggtggcctt gcggaacgcc
     2281 ggccccgagg tcgtccggac ggagaatacg gtccggaccg tccgcgagcg gctgcgtttt
     2341 cttctcgggc gtgacaggac ggaggtggac gcgaaaggag atctcgcggt cgcggtggtc
     2401 gatcctccga actacgacat ggcgctcggg acggccctgg aacatcgccc cgaactgtcc
     2461 gacctgcgga atcgccaggg agtggcggag gagctgctgg tgatcgcccg cgccgggaac
     2521 cgtccccggc tggacttccg gggcgctctc ggctggcagg agatcgactt cggggtcgtc
     2581 gacctcaccg gaaaaacatg gtccgtcggc ctgttcgcct cctggcctct tttcgacggg
     2641 ttccggaccc gcgggcgcgt cgcccaggcc ggcagcgacg tacggaccct tcggatcgag
     2701 gaggcgcaac tcctggacgc catcgccctc gaagtgcgcg acgcggcgaa cgcggtccgg
     2761 gaagcggggg agatcgtgaa cgccctcgcc ggaacggtgg accaggccga ccggttgctg
     2821 gcgatggcgg agaaagggta cgaatacggc gtgaagaccc gcctcgacgt cgacgacgcc
     2881 cagctcaacc gcagccgggc gctcgggaac cttgcgcggg cccggaggga ctaccttgtg
     2941 gccggggcgg cgctgcggca tgccatggga acgctcggag acgggatcgt cccgccgaag
     3001 ggggagaagc aggcattccg gcctgcagcg tctccggtgg gactggtcgg cgaggtgttg
     3061 aacggccggc ccgtcctccc gtaaccgggg gaacctgccg ccccagtacg agctccgcgc
     3121 tgccggggta cccccgcagt aggacaaacg gggggtacga atccttggaa ctacgtgcgc
     3181 tggaggggtt tgcgggggcc tttccgttcc gcttccgcgg ccttgcgggc ttcctcctcc
     3241 cggcgatctt ccgacttgcg gcggtcgagc tggacgccga agaagccgat cccggcgatg
     3301 caggcggcga tcaccgcgag gtaggaaagc atggtgacca gggtggtggt cttggcggcg
     3361 tcgtcgcccc cttcgtagta gagaaggaga ccgtaggcgg aaaaggcggt gaagacggcc
     3421 atcgagatgt tcagcccgtg gatggccatt cttttggaaa cctgcaacgt cggacctctt
     3481 cctggaagcg atccggtgat tctaccacga accggcaacc cctaccggaa gtgacggttg
     3541 atctttccct tcagcctcaa gaccgcctgc gcgtgaagct ggcagacgcg gggctc
//
LOCUS       DASVJL010000095         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_765227, whole genome shotgun sequence.
ACCESSION   DASVJL010000095 DASVJL010000000
VERSION     DASVJL010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765227"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VF357_01780"
     CDS             <1..239
                     /locus_tag="VF357_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEX7522904.1"
                     /translation="ASSARASSPPKSAGAVIRLFDGRYGPYLTDGVVNATVPKGVTLE
                     SITLETALELLSEKAAKGPSKRPAKRSRAPKRKK"
     gene            complement(352..768)
                     /locus_tag="VF357_01785"
     CDS             complement(352..768)
                     /locus_tag="VF357_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7522905.1"
                     /translation="MEFVVVNLTDGQREEEDMARDTEKKDFAKADEVRVFPKGKVELI
                     TIGGATIGRATLEPGWRWSSSVQPIAKTKSCEAPHFQYHIAGTLKVVMDDGTELVCKP
                     GNVSLLPSGHDAWVVGNENVVIVDFQGMVDYAKKAA"
     gene            complement(843..>1758)
                     /locus_tag="VF357_01790"
     CDS             complement(843..>1758)
                     /locus_tag="VF357_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7522906.1"
                     /translation="RQPMFDVVGIGLNAVDYLVGLPKFPVPGEKLRMSAFAREGGGQV
                     ATALVALSRWGCRCKYVGNLGDDEHGRLSSLLLSREGVDLAHVRTVPGASSQFAVILV
                     EEETGERTILWDRDPRIRVSPEDLPLDDIRRARALLLDGHDVPPAITAARTARAAGVP
                     IVLDAEKVQEGTEELLSLCDHIVAAGHFPGLLLPGASAEDGAREILRRYAPRTSTVTL
                     GPRGAFGCDGRDEIFSPSIRVETVDTTGAGDIFHAGFLYALLGGLPFREILAFANTAA
                     GLSCRGMGGRAAIPTIEAIRAADASPGR"
BASE COUNT          328 a          589 c          524 g          317 t
ORIGIN      
        1 ccgctagcag cgcgagagcc tcgtcccccc cgaagtcggc cggggcggtc attcgattat
       61 tcgacgggcg atacggacca tacctcaccg atggtgtcgt caatgccacg gtgccgaaag
      121 gtgtcacgct cgaatcgatc actctcgaaa ccgccttgga gttattgtcc gagaaggcgg
      181 cgaaggggcc ctcgaaaaga ccggcgaaaa gatcccgtgc ccccaagagg aaaaagtgat
      241 cataatccgg tgagttcacg cccgatggag tgtctcatcc aatgaaatgg aggcggggtc
      301 agaccccgcc tcctgcgtac ccacggctat tccggtggcc accgactcga atcaggcggc
      361 cttctttgcg taatccacca tcccctggaa gtcgacgatc acgacatttt cgtttccgac
      421 cacccaggca tcatgcccgg atggcaggag cgacacattg cctggtttgc acacgagttc
      481 cgttccgtca tccatcacga ctttcagggt ccctgctatg tgatactgga aatggggcgc
      541 ctcacaactc tttgtctttg cgatcggctg aacggacgac gaccaccgcc atcccggttc
      601 caatgtggca cgcccaatgg tcgctccgcc gatcgttatc aactccactt tgcccttggg
      661 gaaaacccgc acttcatcgg ccttggcgaa gtctttcttt tccgtatccc gtgccatatc
      721 ctcctcctcc ctctgtccgt ccgtaaggtt gaccacgacg aattccattg tccttacgga
      781 ttggatgagg tggaggtcct gaagatccat ctcgggagca aataatgatc gcggtgacct
      841 tcctaccggc ccggcgatgc gtcggccgcc cggatcgcct cgatcgtcgg gattgcagcc
      901 cgtcccccca ttccgcggca ggagaggccg gctgcggtgt tggcgaaggc gaggatctcc
      961 cggaagggaa gccctccgag gagggcgtac aggaagccgg cgtggaagat gtctccggcg
     1021 cccgtagtgt ccaccgtctc gacgcggatg gaaggcgaga agatctcatc ccggccgtcg
     1081 catccgaacg ccccgcgggg gccaagcgtc accgtggacg tcctgggtgc ataacggcgc
     1141 aggatctccc gggcgccatc ttccgccgag gccccgggaa ggagcaaccc cgggaagtgt
     1201 cccgcggcca cgatgtgatc gcacagggac agcagttcct ccgtcccctc ctgcaccttt
     1261 tccgcgtcga gaacgatggg gacgcccgcg gcccgggcgg tcctcgccgc ggtaatcgcc
     1321 ggggggacgt cgtgaccgtc gagaaggagc gcccgcgccc gccggatgtc gtcaagcggc
     1381 aggtcttcgg gtgatacccg gatccgcgga tcccggtccc acaggatggt ccgctcaccc
     1441 gtttcttcct ccacgaggat caccgcgaac tgggacgagg cgccgggaac cgtccggacg
     1501 tgggcgaggt cgactccctc ccgcgaaagg agcagggacg aaagccgtcc gtgctcgtcg
     1561 tctcccagat tcccgacata tttgcaccgg cacccccaac gggatagcgc cacgagggcg
     1621 gttgccacct ggccgccgcc ctctcgcgcg aaagcggaca tccgcagctt ctcgcccggc
     1681 accgggaact tcggcaggcc cacaaggtaa tcgaccgcat tgagtccgat cccgacaacg
     1741 tcgaacatcg gttgcctc
//
LOCUS       DASVJL010000096         6104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1608838, whole genome shotgun sequence.
ACCESSION   DASVJL010000096 DASVJL010000000
VERSION     DASVJL010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608838"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VF357_01795"
     CDS             <1..431
                     /locus_tag="VF357_01795"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX7522907.1"
                     /translation="LDRIAIPSANVHPMPVEEEDLDEEARRYGSILRRIAGTPPVLDL
                     VQLGLGEDGHTASLFPGDAALRIVDADVAVSGLYKGRRRMTLTFPAIDRARCILWLVT
                     GSGKIEALGRLRSGDRSIPAGRVRSDRAVLLADKTAEGIG"
     gene            complement(485..1492)
                     /gene="arsS"
                     /locus_tag="VF357_01800"
     CDS             complement(485..1492)
                     /gene="arsS"
                     /locus_tag="VF357_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551585.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEX7522908.1"
                     /translation="MAEGIVSGIRERLPGIGTGAKEAAGVEPFADILVRHGLSLLRDR
                     VHTLQVNTGYLCDLRCRHCHLEAGPGRMEIMSRETMETVVSFVRRFPFQILDITGGAP
                     ELVPDLPFLVEGLAPLAPRLMLRTNLSALRGAARESLLALCVAHRVVLVASFPSTNPS
                     QADALRGAGVTETAIAVLKKLNAAGYGVEGTGLELDLVSNPVGAFLPVSQESAERKFR
                     NDLLRKWGIVFNHLYTFANVPLGRFRTWLLQTENYERYLKTLTDGFNPSAVERLMCRT
                     LLSVSWDGFLYDCDFNLAAGRPSADRMIHVSGVRELPPPGAPIAVGDYCYACTAGSGF
                     T"
     gene            complement(1511..1843)
                     /locus_tag="VF357_01805"
     CDS             complement(1511..1843)
                     /locus_tag="VF357_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HEX7522909.1"
                     /translation="MKTYYDPADLATFGEIGKDAPELGKKFFEYYAEVFKEGELTERE
                     KALIALGVAHAVQCPYCIDAYTRACLEKGSNLGEMTEAVHVAAAIRGGASLVHGVQMR
                     KIAEKLSM"
     gene            complement(1940..2650)
                     /locus_tag="VF357_01810"
     CDS             complement(1940..2650)
                     /locus_tag="VF357_01810"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEX7522910.1"
                     /translation="MKARKILILFVFAAVVAVFHFLDLGKYLTLESLKANRALLADLR
                     GAHTFVFAAVFVLIYILQTAFSLPGAAILSLAAGAIFGVLQGTLFVVSGATVGAILAF
                     LVSRTLLRDWVVRKFGGRMEGIDRGLRENGLSYLLFLRLVPAFPFFLVNLACGVTGLP
                     LRTYAVGTLFGIMPGSLVFVNAGASLAAIESVRQVASPRVLGSFALLGLFALLPTIIK
                     AVKKRREGNPLPFDRRGK"
     gene            complement(2647..4134)
                     /locus_tag="VF357_01815"
     CDS             complement(2647..4134)
                     /locus_tag="VF357_01815"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7522911.1"
                     /translation="MEAIMGAYDYDLGVLGGGAAGLTAAAGAAQFGAKTILVEKAKKL
                     GGDCLHFGCVPSKTLIHTAGVWSLARRAKEFGLPDVELPPVSLAAVMDRVRSVIDTIQ
                     EHDSPERFCKLGAEVRFGSPRFVDDHTVDLGGARLTARAWIIATGSSPSLPPVEGIAE
                     VPYWTNETVFSQTELPGRLLILGGGPIGVEMAQAFRRLGSGVTIIEYGDQVLGREDPD
                     IASILRSRLEAEGVKILTDTKVLKAATANSSVLLRVAPAKGEGEPWTIEGDVLLVAAG
                     RKPDVEGLELSAAGVDFSPRGVPADRRMRTNVPHIYSCGDVNGVFPFTHVAGYEAGIA
                     LTNAVLRLPRKADYTKVPWCTYTDPEVASVGLNEKRAKTAGVEYRTLEVPFRDVDRAL
                     AEGEAGGKIKVLISPSGALLGCQIAGHHAGELIHEWVAAMNGGVKLSSLAGAIHAYPT
                     LSEISKKAAGSYYSEKLFSDRTKKILRFLFGLKGRACTPEEEVSG"
     gene            4251..4787
                     /locus_tag="VF357_01820"
     CDS             4251..4787
                     /locus_tag="VF357_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_241392852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4374..4376,aa:Sec)
                     /transl_table=11
                     /product="SelL-related redox protein"
                     /protein_id="HEX7522912.1"
                     /translation="MSDLTGMPAIPFQLRDSTGRSHLLEEYRGSWLLMVFHRHLGULP
                     CRGHITQLRDREEEFRRRKVRIVVVTFENDFLARAYAEDTSLTWPLLVDDTRETYRAY
                     GMLSASFWDIWGPKTWWAYLKEILKGERMRKSGGDIFQRGGDVLIDPNGIVLLHHIGA
                     GPSDRPPVESILQKIHPA"
     gene            complement(4829..5650)
                     /locus_tag="VF357_01825"
     CDS             complement(4829..5650)
                     /locus_tag="VF357_01825"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX7522913.1"
                     /translation="MRNLLGIIGSPRKMGNCELMVKEIAAAVAGSPKLLMVRLVEKDI
                     RPCKACYRCLTGDCPHQDDFSGVLRAIIEADAVIVAAPAYLRGTHSSLQRFLDRGLQA
                     YRHVDALYGKPAVAVATAGVEDGEGTALLGVENFIRQLGLSLKGRAVVRATFPGDAIV
                     SVEGRSAAHRLAAALVSTDDYTPDGVSCPECKGTYFEFRGMAAIYCLSCGGTGSFSVD
                     GGQVDLAIGPPAHSWRKKEDMASHGQWLIGRREEFLRQRGRLKDAVKPYLGGEFL"
     gene            complement(5656..>6104)
                     /locus_tag="VF357_01830"
     CDS             complement(5656..>6104)
                     /locus_tag="VF357_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7522914.1"
                     /translation="FQRKPAPKEWSAYNCLRHLVDTERSVFPARVGYLLRGEDFPAFH
                     PDEEGKGLGGNITQFELAKEFARLRAGSLAQLSKVGEGDLSRSARHQELGMVTLGEMI
                     HEWAGHDLMHTVQGERAILQPFIDGSGPWKRYFSDHTIPPSPQGGR"
BASE COUNT         1097 a         2077 c         1857 g         1073 t
ORIGIN      
        1 tgctcgatcg catcgcgatc ccctccgcca acgtccatcc catgccggtg gaagaggagg
       61 atctcgacga ggaggctcgc cgctacggat cgattcttcg ccggattgcc ggaacgccgc
      121 cggttctcga cctcgtccag ctcggactgg gggaggatgg ccacacggcc tcccttttcc
      181 cgggcgacgc cgcactccgg atcgtcgacg cggacgtcgc cgtcagcggc ctttacaagg
      241 ggcgaaggcg gatgacgctc acctttccgg cgatcgatcg ggcccggtgc atcctgtggc
      301 tggtcaccgg ttccgggaag atcgaagcgt tggggcggtt gcgctcaggc gaccgatcga
      361 ttccggcggg acgcgttcga agcgaccgtg ccgtcctcct tgcggacaag accgccgagg
      421 gaatcggata accgggcggt gccgcgttgt ccgccggccc ctacggggcc gatatcgcgc
      481 cgcctcaagt gaagccggag cccgcggtgc aggcgtagca gtagtcccct accgcgatcg
      541 gggcgccggg cggaggcagt tcgcggaccc ccgaaacgtg gatcatgcgg tcggcggaag
      601 gtcgcccggc tgccaggttg aaatcgcagt cgtacaggaa cccgtcccag gagacggaaa
      661 ggagcgttcg gcacatgagc ctttccaccg cggaggggtt gaagccgtcc gtcagcgttt
      721 tcagataccg ttcgtagttt tccgtctgca gcagccacgt ccggaaacgt cccagcggta
      781 cgttcgcgaa cgtgtacagg tggttgaaca cgatccccca tttccgcagc aggtcgttcc
      841 ggaatttccg ttcggcggac tcctgggaaa cgggaaggaa cgccccgacc gggttggaga
      901 cgaggtccag ttccagcccg gtcccttcca ctccataccc cgccgcgttc aacttcttca
      961 gcacggcgat tgcggtttcc gtcacccccg cgccacgcag ggcatcggcc tgggacggat
     1021 tcgtcgaagg gaaggaggcg accaggacga cgcggtgggc gacgcagagc gcgagaagcg
     1081 actccctcgc ggcgcccctc aaagcggaaa ggttcgtgcg aagcatcagc cggggcgcaa
     1141 gcggggcaag cccttcgacg agaaaaggaa ggtcgggcac cagttccggc gcgccgccgg
     1201 tgatatcgag gatctggaac gggaatcgcc ggacgaacga gacgactgtc tccatcgtct
     1261 cccgggacat gatctccatc ctgcccggac cggcctcgag gtggcaatgc cggcaacgga
     1321 gatcgcacag gtaccccgtg ttcacctgaa gggtgtggac gcggtcccgg agcagggaaa
     1381 gtccatgacg cacgaggatg tcggcgaacg gctcgacccc tgccgcctcc ttggcgccgg
     1441 tcccgatccc cggcaacctt tccctgatcc ccgacacgat gccttcggcc aattcgattc
     1501 tgtcccttgc ctacatggaa agcttttcgg cgatcttccg catttgcacc ccgtgcacga
     1561 gggacgcgcc tccacggatc gccgccgcca catggaccgc ctcggtcatc tctccgaggt
     1621 tggaaccttt ctccaggcag gcgcgcgtgt aggcgtcgat gcagtagggg cattggaccg
     1681 cgtgcgccac cccgagggcg atcagcgctt tctcccgctc cgtgagctcc ccctccttga
     1741 acacctccgc ataatactcg aaaaacttct tcccgagctc cggcgcgtcc ttcccgatct
     1801 cgccgaacgt cgcgagatcc gcgggatcat aatacgtctt catggatacc ctccttatcg
     1861 tgaccgcccg aggttcgccg gtcgatcgtt tccggtttcc gatccgcccc atgcgaggcc
     1921 cgctttcggg tcataataat cacttccctc tccgatcgaa agggagggga ttcccctccc
     1981 ggcgcttctt cacggccttt atgatcgtgg ggagcagggc gaacaaccca agcagtgcga
     2041 acgatcccag cacccgaggg gatgcgactt ggcgcacact ctcgatcgcc gcaagactcg
     2101 cccccgcgtt cacgaagacc aggctccccg gcatgatccc gaacagcgtc ccaacggcgt
     2161 aggtccgaag cggcagtccc gtgacgccgc acgcgaggtt gaccaggaag aacgggaatg
     2221 ccgggaccag ccgcaggaag agaaggtagg agagcccgtt ttcccgcaac ccgcggtcga
     2281 tcccctccat ccgaccgccg aacttcctca ccacccagtc ccgcagcagc gtccggctga
     2341 cgaggaaggc gaggatggct ccgacggttg cgcccgacac cacgaacagg gtcccctgga
     2401 ggaccccgaa gatcgctccc gcggcaagcg agaggatggc cgcccccggg agggagaagg
     2461 ccgtctggag gatgtagatc aacacgaaga cggcggcgaa cacgaacgta tgcgccccgc
     2521 gcagatccgc aaggagcgcc cggttcgcct tcaggctttc gagcgtcaga tacttcccca
     2581 ggtcgaggaa gtggaacacc gccacgaccg cggcgaatac gaagaggatc aggatcttcc
     2641 gggccttcat ccggacacct cttcctccgg cgtgcacgcc ctccccttca ggccgaagag
     2701 gaagcgcagg atcttcttcg tccggtcgct gaacagcttc tccgagtaat acgacccggc
     2761 ggctttcttc gagatttccg aaagggtcgg gtaggcgtgg atcgctcccg ccaaagagga
     2821 gagcttcacc cccccattca tggcggcgac ccattcgtgg atcagttccc cggcatgatg
     2881 cccggcgatc tggcatccaa gaagcgcccc ggacgggctg atcagcacct tgatcttccc
     2941 tccggcctcc ccttcggcaa gggcgcggtc cacgtctcgg aaaggcactt cgagggtccg
     3001 gtattccacc cccgccgttt tcgcccgctt ctcgttcagc cccacgctcg ccacctcggg
     3061 atcggtgtac gtgcaccacg ggaccttcgt gtagtccgcc ttcctcggca accggagcac
     3121 cgcgttggtc agggcgatcc ccgcctcgta cccggccacg tgggtgaacg ggaagacgcc
     3181 gttcacgtcc ccgcacgaat agatgtgggg aacgttcgtc cgcatccgcc ggtcggccgg
     3241 aaccccgcgg ggagagaagt cgacccccgc cgcggataac tccaatccct ccacgtccgg
     3301 tttccgtccg gccgccacaa ggaggacgtc cccctcgatc gtccacggtt ccccctcccc
     3361 tttcgccggc gcgacgcgca acaggacgga ggaattcgcc gtggccgcct tgaggacctt
     3421 cgtgtcggta aggatcttca ctccctcggc ttccaggcgg cttcggagga tcgaggcgat
     3481 gtccggatcc tcgcgcccga gaacctggtc cccgtattcg atgatggtga cgcccgaccc
     3541 gagacgccgg aacgcctggg ccatctcgac gccgatcggc ccgccgccga ggatcagcag
     3601 gcgtcccggc agctccgtct gcgagaagac cgtctcgttc gtccagtacg gtacctccgc
     3661 gatcccttcg acgggaggca gggagggact cgagccggtg gcgatgatcc atgccctcgc
     3721 cgtcaggcgc gcccccccga ggtcgaccgt atgatcgtcg acgaagcgcg gggatccgaa
     3781 ccggacttcc gcgccgagct tgcagaaccg ttccggtgag tcgtgctcct ggatcgtgtc
     3841 gatcaccgag cggacccggt ccatgacggc ggcgagggac accggcggaa gctccacatc
     3901 cggaagcccg aactccttcg cacggcgggc gagggaccat acgccggccg tatggatcag
     3961 cgtcttcgac gggacgcagc cgaagtggag gcagtcgccc cccagtttct tcgccttttc
     4021 gacgaggatc gtcttcgcgc cgaactgcgc cgcccccgcc gccgccgtga gccccgccgc
     4081 gcctcctccg agaaccccca gatcgtaatc gtacgccccc atgatcgcct ccatcttcac
     4141 ccttcttcac cagcacattg tgtccgaagg gtgccaaggg tccataatcg atatccatgg
     4201 gatgcaacat tttgcgacga gggagcgacg tatgtacgcg gtagtttgcc atgagtgacc
     4261 taaccggaat gcccgccatc cctttccagc tccgggacag taccggccga agtcacctcc
     4321 tcgaggagta ccgtggaagt tggctgttga tggtgtttca tcgccatctc ggctgactcc
     4381 cttgccgggg gcacatcacg cagttgcgtg atcgggaaga ggagtttcgg cggcggaagg
     4441 ttcgaatcgt tgtggtcacg ttcgaaaacg acttcctcgc ccgcgcgtat gccgaggata
     4501 cttcgttgac ctggcccctt ctcgtggacg atacccgcga aacgtacagg gcctacggta
     4561 tgctctccgc ttccttttgg gacatatggg gtccgaaaac ctggtgggct tatctgaagg
     4621 agatcctgaa gggagagaga atgcggaagt caggagggga tatcttccag cgtggcgggg
     4681 atgtcctgat cgatccgaat ggaatcgtcc tcctccatca tatcggcgcc ggcccgtcgg
     4741 atcgtccccc ggtcgagagt atattgcaaa agatccatcc tgcatgaaga ggagtgcgtc
     4801 tcccgcgtct ccctgccgtc tttccgcttc agaggaactc tcccccgagg tacggcttca
     4861 cggcgtcctt caaccggccc ctctggcgga ggaactcctc ccgccggccg atcaaccact
     4921 gcccgtgcga cgccatgtcc tccttcttcc gccaggagtg ggcaggggga ccgatcgcaa
     4981 gatcgacttg tccgccgtcc acggaaaagg atccggtgcc cccgcaggag agacagtaaa
     5041 tggcggccat cccccggaat tcgaaatacg tccccttgca ttcgggacag gagaccccgt
     5101 cgggagtgta gtcgtctgtg gaaacgaggg cggccgccag ccggtgtgcg gcgctcctgc
     5161 cttccacaga aacgatcgcg tctccgggga aggtggcgcg cacaacggct ctccccttca
     5221 gggataaccc cagctggcgg atgaagtttt cgacccccag cagcgccgtc ccctcgccat
     5281 cctcgacgcc cgccgttgcg acggcgaccg ccggttttcc gtacagggcg tccacgtgcc
     5341 ggtacgcctg gaggccgcgg tccagaaacc gctggaggga ggaatgggtg ccccggaggt
     5401 acgccggcgc ggcgacgatc acggcgtccg cttcgatgat tgcgcggagg actccggaaa
     5461 aatcgtcctg gtgggggcaa tccccggtca ggcaccggta acacgccttg cagggacgga
     5521 tatccttttc gacgaggcgc accatcaaga gtttcggaga cccggcgaca gcggcggcga
     5581 tctccttcac catcaactcg cagttcccca tcttccgcgg ggaaccgatg atcccgagca
     5641 gatttctcat ctccgttacc gacctccttg cggcgaaggc gggatggtat ggtccgaaaa
     5701 ataccgtttc caggggccgc tgccgtcgat gaacggctgc aggatcgccc tctcgccctg
     5761 gaccgtgtgc atcaggtcat gccctgccca ctcgtggatc atctccccga gggtcaccat
     5821 tcccaactcc tggtggcgcg ccgagcggct caaatcgcct tccccgacct tcgacaactg
     5881 ggccaggctc cccgcccgca gccgtgcgaa ttccttggcc aattcgaact gggtgatgtt
     5941 acccccgagg cctttcccct cttcatccgg gtggaacgcc gggaaatcct ctccgcgcaa
     6001 cagataaccg acacgggccg ggaacaccga tctctccgtg tccacgaggt gccgtaaaca
     6061 gttgtatgcc gaccactcct tcggcgccgg cttcctctga aaca
//
LOCUS       DASVJL010000097         1861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_202790, whole genome shotgun sequence.
ACCESSION   DASVJL010000097 DASVJL010000000
VERSION     DASVJL010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202790"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VF357_01835"
     CDS             <1..456
                     /locus_tag="VF357_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HEX7522915.1"
                     /translation="ASCNTCHMPKVRNAKGTVYTSHWQTSPRNYLKQTCLTSKCHPAL
                     TEAQANYEIDSVRNFTKGKMRKAEFWLSALIDKIVEGKKAGLPPEVIKEAQEQHQKAH
                     VLWEWWTAENSDGFHNPALARESLTRSVEESRKGIKMIGDALEKKTASK"
     gene            complement(470..1153)
                     /locus_tag="VF357_01840"
     CDS             complement(470..1153)
                     /locus_tag="VF357_01840"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7522916.1"
                     /translation="MRLRSWRRVLPLLVAFLLAGGCSRIPRIIVLSDPLNAAEHVELG
                     VAYERKGELDLARREYERALQKDRKFYRARVNLGNIFLAKKEYGKAREEYLLALELRP
                     GDADATNNLSWAAIFSGERIDEALARMESVVSGPAPPVSPAAGVPQQREARTGAGRDP
                     ILLDTLGVLRMRANHPGTAEEAFAMADALCRQAGTASREGVNVDAPCPDEVRREIKDH
                     REELRRRFP"
     gene            complement(1165..1797)
                     /locus_tag="VF357_01845"
     CDS             complement(1165..1797)
                     /locus_tag="VF357_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEX7522917.1"
                     /translation="MTRSMNTPYVHVTVVKKGFSLIAAALLTGGVLFTQGGCAVGTSA
                     GKGNGAVQSSEAPAPLPSVIAGVPFLPQEDDTCGPSSLAMVLRFLGQNVDTAEIVRET
                     RTEGLKGTLITDLTSAARRRGFEAEVADLDLPRLRERISAGVPVILLVDLGIWSWSRP
                     HYLVAYGWTPEGVVAHSGRERGKVIPFSTLDAQWAKMGRLAIIVRRPGPR"
BASE COUNT          344 a          625 c          599 g          293 t
ORIGIN      
        1 gcgagctgca acacctgcca catgccgaag gtgcgcaacg cgaaggggac ggtgtacacc
       61 tcccactggc agacgagccc gcggaactac ctgaagcaga cgtgcctcac gtcgaagtgc
      121 caccctgccc ttacggaggc gcaggcgaac tacgagatcg actccgtccg gaacttcacc
      181 aaggggaaga tgcgcaaggc ggaattctgg ctctcggccc tgatcgacaa gatcgtcgag
      241 gggaagaaag cggggctgcc gcccgaggtg atcaaggagg cgcaggagca gcaccagaag
      301 gcgcacgttc tctgggagtg gtggacggcc gagaactccg acggctttca caacccggcc
      361 ctggcccgcg agtcgctgac aaggtcggtc gaggagtcgc gcaaggggat caagatgatc
      421 ggcgacgctc tcgagaagaa gaccgcgtcg aagtaacggg ttgagtcccc taggggaatc
      481 gcctccgcaa ttcctcgcgg tgatccttga tctcccggcg cacctcgtcc gggcaaggtg
      541 cgtcgacgtt cacgccctcc ctcgaggcgg taccggcctg ccggcacagc gcgtcggcca
      601 tagcgaaggc ctcctccgcc gtcccggggt ggtttgcgcg catccggaga acacccagcg
      661 tgtccagaag gattgggtca cgccccgccc cagtacgagc ttcgcgctgc tggggtaccc
      721 ccgcagcagg agaaacgggg ggggcaggtc cggagaccac cgattccatc cgggccagcg
      781 cctcgtcgat ccgctctccg gagaagatgg cggcccagga gaggttgttg gtcgcgtcgg
      841 catcccccgg tcgcagttcc agggccagga ggtactcctc gcgggctttt ccgtactcct
      901 tcttcgccag gaaaatattg ccgagattga cgcgggcccg gtagaacttc ctgtccttct
      961 ggagggctcg ttcgtattcc cgccgggcga ggtcgagctc tcccttccgc tcataggcga
     1021 cgccgagttc gacatgctcc gcggcgttca gcggatcgga aaggacgatg atcctcggaa
     1081 tgcgggagca cccgccggcc agcaggaagg cgaccaggag cggcagcacg cgccgccatg
     1141 accgaagacg cacttcttcc ggcattaccg cggtcccggc cggcgaacga tgatcgccag
     1201 ccgccccatc ttcgcccact gcgcatccag cgtggagaac gggatcactt tcccccgctc
     1261 gcgtccggaa tgcgcgacca ccccttccgg agtccagccg tacgccacca ggtaatgggg
     1321 acggctccag ctccagatac cgaggtcgac cagaaggatc acggggacgc ccgccgaaat
     1381 ccgctcccgc agccggggga ggtcgaggtc cgcgacctcc gcctcaaacc cccgccgtcg
     1441 ggcggcactg gtcaggtcgg tgatgagcgt ccccttcagt ccttctgtcc gggtctcccg
     1501 gacgatttcc gccgtatcga cattctgccc caggaatcgg agaaccatcg cgagggagga
     1561 ggggccgcag gtatcgtctt cctgcggaag gaacgggacc cctgcgatga ccgaaggaag
     1621 cggggccggc gcctccgagg attggacggc tccgttccct ttccccgcgc tcgtgcccac
     1681 cgcgcatccc ccctgcgtga agaggacacc gcccgtcagc agggcggcgg cgataaggga
     1741 gaaccctttt ttcaccacgg taacgtggac gtagggcgta ttcatggaac gcgtcacttg
     1801 gtgacgtccc tctcctcgac gaccacgttt ccccgatgga tcacgaggga gatccgccca
     1861 t
//
LOCUS       DASVJL010000098         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_577747, whole genome shotgun sequence.
ACCESSION   DASVJL010000098 DASVJL010000000
VERSION     DASVJL010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577747"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VF357_01850"
     CDS             <1..366
                     /locus_tag="VF357_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7522918.1"
                     /translation="YTWFYRLVVNQAIDRWRKSARSAEVPFDESWLSEDASPPDAFAY
                     PRTPEDLVRDRELGDALQRAVDSLPGYHRAVIVLREVDGMAYDEIAKVLGCSVGTVMS
                     RLHYARGKLKETLKAHREG"
     gene            380..856
                     /locus_tag="VF357_01855"
     CDS             380..856
                     /locus_tag="VF357_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7522919.1"
                     /translation="MDCRTALEKIEAKADGLLPKEAEAELDGHLAGCTACTVEDAAIS
                     ATGPVLRALTGIRAREKGPALDAMWTRVRAGIEESRAARRRSSWITRWAWIPAAVAIA
                     VFALLFYPVRTDRSPFHPSTFDVSVEDVESDSATVALVDKGEDLPRVIWIIEDAKT"
     gene            843..1307
                     /locus_tag="VF357_01860"
     CDS             843..1307
                     /locus_tag="VF357_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522920.1"
                     /translation="MPRPEPGGRAVAPALSFLLLLLLLAAVPSEAANMVTVDVGAVYA
                     SNEGTSIDPDLGAIRGKLRSMFNYTSYRMLDRKRRSLSVGEAGEFELPDRRTMRAIPL
                     PARGDKVRLSIQISEGPRNLLTTTLGLRRGGMVLVGGPSHRAGVLILIISAE"
BASE COUNT          241 a          502 c          558 g          268 t
ORIGIN      
        1 tacacctggt tctaccgcct cgtcgtgaac caggcgatcg accggtggcg gaagtcggcg
       61 cggtcggcgg aggtgccctt tgacgagagc tggctttccg aggatgcctc gcccccggac
      121 gccttcgcgt acccgcggac ccctgaggac cttgtccggg accgtgagct gggggacgcc
      181 ctccagcgcg ccgtcgactc cctccccggg tatcaccggg cggtgatcgt ccttcgggag
      241 gtggatggga tggcgtacga tgagatcgcc aaggtcctcg ggtgttcggt gggaacggtg
      301 atgtcccggc tgcactacgc gcgcgggaaa ctgaaagaga ccctgaaggc gcacagggaa
      361 gggtagggaa gggacgacga tggattgccg gacggctctt gaaaagatcg aggcgaaggc
      421 cgatggcctc ctcccgaagg aggcggaagc ggagcttgac ggccacctgg ccgggtgcac
      481 cgcgtgcacc gtggaagatg ccgcgatctc cgcgacagga cccgtgctgc gcgccttgac
      541 cgggatccgg gcaagagaga aaggtcccgc tctcgacgcg atgtggacgc gggtgcgcgc
      601 cgggatcgag gagagccggg cggcgcggcg gcgctcctcg tggatcacgc ggtgggcgtg
      661 gattcccgcg gccgttgcca ttgcggtgtt tgcgctcctg ttctaccctg tccggacgga
      721 tcggtcgccg tttcacccga gcacgttcga cgtctccgtc gaggacgtgg aatccgattc
      781 cgccacagtg gcgctcgtcg acaagggaga ggaccttccg agggtgatct ggatcatcga
      841 agatgccaag acctgagccg ggcggccggg cggtcgcgcc ggcgctttcc ttcctcctgc
      901 tcctgctgct gctcgccgcc gtcccgtccg aagccgccaa tatggttacc gtcgacgtcg
      961 gggcggtcta cgcctccaac gaggggacct cgatcgatcc ggacctcggg gcgatccggg
     1021 ggaaactccg ttcgatgttc aactacacct cctaccggat gctggaccgc aagcgtcgct
     1081 ccctgtcggt cggggaggcc ggggagttcg agctccccga ccggcgcacg atgcgggcga
     1141 tccctctgcc ggcccggggg gacaaggtcc gcctctccat acagatctcc gaggggccga
     1201 ggaatctcct caccaccacc ctcgggctcc gccggggcgg catggtcctc gtcggtggac
     1261 cgtcccaccg ggccggcgtc ctgatcctca tcatttccgc ggagtagacc cgggaggatc
     1321 cttctccgga tggttgaata tcctccgctc cccggccgtc gaaggattaa gaacgggatg
     1381 gagggagacg accatgaaga aactgatcgc cataatgctg acggtgggac ttctcgcggc
     1441 cccgatggcc gcgttggcgg gcgggcgcgg cggctactcc ggccggggcg gctattccgg
     1501 ccacggcggc tacggtgtgg ggggattctt cgctggactg ctgggagggg ccatcctcgg
     1561 taccgtcct
//
LOCUS       DASVJL010000099         3361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_765833, whole genome shotgun sequence.
ACCESSION   DASVJL010000099 DASVJL010000000
VERSION     DASVJL010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765833"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..132)
                     /locus_tag="VF357_01865"
     tRNA            complement(59..132)
                     /locus_tag="VF357_01865"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(98..100),aa:Gln,seq:ctg)
     gene            complement(311..1699)
                     /gene="gltX"
                     /locus_tag="VF357_01870"
     CDS             complement(311..1699)
                     /gene="gltX"
                     /locus_tag="VF357_01870"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX7522921.1"
                     /translation="MPNVVTRFAPSPTGFLHIGGARTALFNLLYARKNGGKFILRVED
                     TDQERSTPEAVRAILDGMTWLGITWDEGPHFQMERMELYRKEAERLLREGKAYRCVCT
                     KEELDARREEMTARKEKPRYDGRCRDLAPGATEGKPSVLRFKTPRTGRTVVHDLLRGD
                     VVYENAELDDLVFLRTDGSPTYNFVVVIDDASMGITHVLRGDDHLNNTPKQVLLYEAL
                     GYPLPQFGHFPLIHGMEGGKLSKRQDDVSVTAYREKGFLPEAMVNYLVRLGWGHGDQE
                     IFSMVEMQDLFTLEHVGKSPSKFNLDKLLHLNAHYIKMAEPSLLANLLVPFLAKRGID
                     AKPSPWLTAAVRTLLERSRTLEEMAGSAEYYFRAKPADPKAASKFLTPEIAPVLLEIA
                     EAFSALEDFNADLEDALKEVVERREGDLKVHQPIRVALTGGTASPGLFEVMRILGRDE
                     VVRRLKSASSRI"
     gene            complement(1755..2558)
                     /gene="amrB"
                     /locus_tag="VF357_01875"
     CDS             complement(1755..2558)
                     /gene="amrB"
                     /locus_tag="VF357_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX7522922.1"
                     /translation="MKRMPAVSGQFYPGTASGLSRALLTLTREAKEPEPAIGVVAPHA
                     GYVYSGAVAGEVFSSVRVPGRAILLCPNHTGIGEDAAIMSHGSWGMPWGDVPVDEELA
                     ARLESACPFLREDASAHSREHAIEVQLPFLHRFRPDVRIVPVALGRLSLEECRELGES
                     VADAIAGDAERPLLVASSDMSHYVPDAVARKKDRLAIDRMLALDPEGLYATVLTERIS
                     MCGVMPATVVLFAARRLGATSARLIKYATSGDVSREFDQVVGYAGLAFR"
     gene            2654..3196
                     /locus_tag="VF357_01880"
     CDS             2654..3196
                     /locus_tag="VF357_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX7522923.1"
                     /translation="MYIRVSEIPGGGLDLFASRGKASIPRVLEGMDPAPLREVRLVDA
                     DLLLTVEGGDLWIEGSFEAQGEGFCDRCSDPVTLRLGKAFQTILTPKSRDRAVAASVE
                     LHEEDLDVGYYDGTGVETNDILWEQVVLELPRKVVCSEACRGVCPVCGKNRNLEECSC
                     VAGDAPGPFEILKNLKGKKE"
BASE COUNT          585 a         1038 c         1160 g          578 t
ORIGIN      
        1 aacccgcgtt ttcgccgtga gagggcggcg tcctgggcca ctagacgatg ggaccggttg
       61 gctggggagc cagggctcga acctggaaac cgtgggtcag agccacgtgt gataccattt
      121 caccactccc caacggaacg accaaggtat ccgatcggga gcagttgcgt caagcgacaa
      181 tccgaccagc gatggccatg gatggccaag tgccgcgatg cccagggagg ggcaagtgcc
      241 gcgggagcca tggacggcgg gagcggccgg gcgcagggat gcgcaggagc ggccggcgct
      301 catatacagg ttatattcga gatgatgcac ttttgaggcg gcgcaccacc tcgtcccgcc
      361 cgaggatcct catcacctcg aacagccccg gggaggcggt cccgccggtg agggcgacgc
      421 gaatcggctg gtggaccttc aggtccccct ccctgcgttc caccacttct ttcagggcat
      481 cctccagatc ggcgttgaag tcctcgaggg cggaaaacgc ttcggcgatc tcgaggagca
      541 ccggggcgat ctccggcgtg aggaacttgg acgccgcctt cggatcggcc ggtttcgcgc
      601 ggaagtaata ttccgcggat ccggccatct cctcgagggt gcgggacctc tccagcaacg
      661 ttcgcacggc ggcggtgagc cacggagaag gttttgcgtc gatccctcgt ttggcgagga
      721 aggggacgag aaggttcgcg agcagcgaag gttccgccat tttgatgtaa tgggcgttga
      781 ggtggaggag tttgtcgagg ttgaatttcg acggagactt gccgacgtgt tccagggtga
      841 agaggtcctg catctccacc atggagaaaa tctcctggtc gccgtggccc caaccgagac
      901 ggacgaggta gttcaccatc gcttcgggaa ggaacccctt ctcccggtac gccgtcaccg
      961 acacgtcatc ctggcgtttc gagagtttcc ctccttccat cccgtggatc aacggaaagt
     1021 ggccgaactg tggaagaggg tagccgagcg cttcataaag gaggacctgt ttcggtgtgt
     1081 tgttcaggtg gtcgtctccc cgcaggacgt gggtgatccc catcgacgca tcgtcgatca
     1141 cgacgacgaa gttgtacgtc ggcgacccat cggtccgcag gaacaccagg tcgtccagtt
     1201 ccgcgttctc gtagaccacg tcgcctcgga gcaggtcgtg caccaccgtc cgtcccgtgc
     1261 gcggtgtctt gaaacggagg acggaaggct tcccctccgt cgctccaggt gcaaggtcgc
     1321 ggcaccggcc gtcgtaccgg ggcttctcct tccgcgcggt catctcctcg cgccgcgcgt
     1381 cgagctcctc tttcgtgcag acgcaccggt acgccttccc ttcccgcagt aaacgttccg
     1441 cctccttccg gtacagctcc atccgctcca tctggaagtg cggtccctcg tcccaggtga
     1501 tgccgagcca cgtcatcccg tcgaggatcg cccggaccgc ctcgggggtg gaccgctcct
     1561 ggtcggtgtc ctcgacccgc aggatgaatt tgccgccgtt tttgcgcgcg tagaggaggt
     1621 tgaacagggc ggtgcgggcg ccgccgatgt ggaggaatcc cgtggggctg ggggcgaagc
     1681 gcgtcacgac gttcggcatg gaaggtcctc gattcggggg atcggttccg gtggacggga
     1741 cggaaaggag agcctcagcg gaaggcgagc cccgcgtagc cgaccacctg gtcgaactcc
     1801 cggctgacgt cgccggaagt ggcgtatttt atcagacggg ccgacgtcgc gccaaggcgg
     1861 cgggcggcga agaggaccac cgtggccggc atcaccccgc acatcgagat ccgctcggtc
     1921 aggacggtcg cgtagagtcc ctccgggtcg agggcgagca tccggtcgat cgccaaccgg
     1981 tccttctttc gcgcgaccgc gtccggcaca tagtgggaca tgtcggagct ggcgaccagc
     2041 aggggacgct ccgcgtctcc cgcgatcgcg tccgccacgc tctccccgag ctcccggcac
     2101 tcctcgaggg agaggcgccc gagggcgacg gggacgatgc gcacgtccgg ccggaaccgg
     2161 tgaaggaacg ggagctggac ttcgatcgcg tgctcccggg aatgggccga cgcgtcttcc
     2221 cgcaggaagg ggcaggcgga ttcgaggcgg gcggcgagct cctcgtccac ggggacgtcc
     2281 ccccacggca tcccccagga accgtgcgac atgatggcgg catcctctcc gatcccggtg
     2341 tggttcgggc agaggaggat ggcgcggccc ggcacccgga cggaggagaa cacttccccc
     2401 gccacggccc ccgaatagac gtatcccgcg tggggggcca cgaccccgat cgccggctcc
     2461 ggctccttcg cctcccgggt gagcgtcagg agcgcccggg aaaggccgga ggcggtcccg
     2521 gggtagaact gtccggagac ggcgggcatc cgcttcatgc ccatatgata caacggggag
     2581 cggggatttg gcgattgaca gcgggggggg ggttccctat agtatctatt ttactttacg
     2641 aggaaattcg cgcttgtaca tccgggtatc cgagattccc gggggcggac tggatctgtt
     2701 cgcttcccgg ggaaaggcgt cgatcccccg cgttctcgaa gggatggacc ccgcgccgtt
     2761 gcgggaggtg cggctggtcg acgcggacct gctcctgacg gtcgagggcg gcgatctctg
     2821 gatcgagggg tcgttcgaag cccaggggga aggtttttgc gaccgttgtt ccgacccggt
     2881 gacgctccgg ctcggaaagg cgttccagac gatcttgacg ccgaagagcc gggatcgggc
     2941 cgtcgccgcc tcggtcgaac ttcatgagga agacctcgat gtcgggtatt atgacggcac
     3001 gggggtcgag acgaacgaca tcctgtggga gcaggtggtc cttgagctcc cgcggaaggt
     3061 cgtttgttcc gaggcgtgcc gcggggtctg ccccgtgtgc gggaagaacc ggaacctgga
     3121 ggaatgctcc tgcgtcgcgg gggatgcccc gggcccgttc gaaattctca agaacctgaa
     3181 aggaaaaaag gagtaagcca tgccgaatcc aaaacgacgc ggatcgaaat gccgccggga
     3241 caagcggcgc acgcacaaga agctgtccca gccggcggtg agcatctgcc cgcagtgcaa
     3301 ggcgaccaag cgtcctcacg ccgtttgccc gacgtgcggg acctacaagg gtcgggaagt
     3361 c
//
LOCUS       DASVJL010000100         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_484487, whole genome shotgun sequence.
ACCESSION   DASVJL010000100 DASVJL010000000
VERSION     DASVJL010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484487"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VF357_01885"
     CDS             <1..633
                     /locus_tag="VF357_01885"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7522924.1"
                     /translation="GSLGATGRFSEESPLAPNSPYAASKTSADLLVRAYGKTFGLPVL
                     VTRCSNNYGPYQFPEKLIPFFVTLLHEGKPVPVYGDGMNVRDWIHVEDHSRAVDAVLR
                     SGRPGETYNVGGGNERTNIEITKLLIAAMGKDERAMKFVPDRPGHDRRYAIDDAKIRA
                     ELGVVPQVPFEEGLRATVRWYIDNEPWWRSVQSGEYQSFYDRWYVGKDRA"
     gene            complement(659..1606)
                     /locus_tag="VF357_01890"
     CDS             complement(659..1606)
                     /locus_tag="VF357_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7522925.1"
                     /translation="MKYLVTGGAGFIGSNLTGALLASGQSVRVLDNFLTGKRENLAGL
                     AEVHGNAFELIEGDLRDLETVRKAAAGIEFVLHQGALPSVPRSVADPVLSNEINVTGT
                     VNLLVAARDAGVRRVVFAASSSAYGDTPQLPKRESMTPNPMSPYAAQKLAGEYYLRIF
                     YEIYGLETVSLRYFNVFGPRQDPKSTYAAVIPRFLTSVHRGVPPTIYGDGLQTRDFTY
                     IDNVIQANLAACLAPKAACGKVVNIACGERVSLLDILEIVYGLAGKRVPPKFEPGRVG
                     DVRDSLADISLARDLIGYDPKVPFSEGLAKTFDWFRSTP"
     gene            complement(1627..>1957)
                     /locus_tag="VF357_01895"
     CDS             complement(1627..>1957)
                     /locus_tag="VF357_01895"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEX7522926.1"
                     /translation="GAKVLVLGVAYKKDISDVRESPALDILQLLAKKGAHLSYCDPYV
                     PEVREAGMTLAASPFSAATLRKADCVVIVTNHAAFDYKMVAREAKVIVDTRNALKGHS
                     GRKIIKL"
BASE COUNT          325 a          635 c          672 g          325 t
ORIGIN      
        1 ggctcactgg gggcgacagg gcgcttctcc gaagagtcgc cgcttgcgcc caactctccc
       61 tacgccgcca gcaagacgtc ggcggacctg ctggtgcggg cgtacgggaa gacgttcggc
      121 ctgccggtgc tcgtcacccg ctgctcgaac aattacggtc cgtaccagtt tcccgagaag
      181 ctcatcccgt tcttcgtgac gctgctgcac gaggggaagc cggtgccggt gtacggcgac
      241 gggatgaacg tgcgcgactg gatccacgtg gaggaccatt cgcgggcggt ggatgcggtg
      301 ctgcggagcg gtcgccccgg cgagacctac aacgtgggcg ggggaaacga gcggacgaac
      361 atcgagatca cgaagcttct gatcgccgcg atggggaagg acgagcgggc gatgaagttc
      421 gttcccgacc ggcccggtca cgatcggcgg tacgcgatcg acgacgcgaa gatccgggcg
      481 gagctgggcg tcgtcccgca ggtcccgttc gaggaggggc tgcgcgccac ggtgcgctgg
      541 tacatcgaca acgaaccgtg gtggcgttcg gtccagtccg gcgagtacca gtcgttctac
      601 gatcgctggt acgtcggcaa ggaccgcgcc tgaccccgga accccgggaa cgtcccggtt
      661 atggggtcga tcggaaccag tcgaacgtct tcgcgagtcc ctcggagaag ggaaccttcg
      721 gatcgtagcc gatgaggtcc cgggcgaggg agatgtcggc cagcgagtcg cgcacgtccc
      781 ccacacgacc cggttcgaat ttcggcggta cgcgcttccc tgccagccca tagacgatct
      841 cgaggatatc aaggagcgac acccgttccc cgcaggcgat gttgacgacc tttccgcacg
      901 cggccttcgg ggcgagacag gccgcaaggt tggcctggat cacgttgtcg atgtaggtga
      961 agtcgcgcgt ctgaagtccg tcgccgtaga tggtgggggg gacccctcgg tgcaccgagg
     1021 tgaggaaccg ggggatgacg gcggcgtacg tcgacttcgg gtcctgccgc gggccgaaca
     1081 cgttgaagta gcgcagggag acggtctcga gcccgtagat ctcgtagaag atgcgcaggt
     1141 aatattcccc cgccagcttc tgcgccgcgt agggggacat cgggttgggg gtcatcgact
     1201 cccgcttggg aagctggggg gtgtccccgt aggcggagga ggatgcggcg aagaccaccc
     1261 gccggacgcc ggcgtcgcgc gcggcgacga gcaggttcac cgtccccgtc acgttgatct
     1321 cgttggagag gaccgggtcg gccacggagc gtggaaccga ggggagcgcc ccctgatgga
     1381 gaacgaactc gatgcctgcc gccgccttgc ggaccgtttc gaggtcccgc aggtcaccct
     1441 cgatcagttc gaaggcgttg ccatgcacct ccgccaaccc ggcgaggttt tcccgtttcc
     1501 cggtgaggaa gttgtcgagg acccgcacgc tttgccccga cgcgaggagg gcccccgtca
     1561 ggttcgaccc gataaacccc gccccgcccg tcaccagata cttcatcccg cccacctccc
     1621 ccgcggtcag agcttgatga tcttgcggcc gctgtgcccc ttcagcgcgt tgcgggtgtc
     1681 gacgatcacc ttcgcctcgc gggcgaccat tttatagtcg aacgcggcgt ggttcgtcac
     1741 gatcaccacg cagtcggcct tccggagcgt cgcggcggaa aacggcgacg cggcgagcgt
     1801 catccccgcc tcccgcacct cgggcacgta cgggtcgcag tacgacaggt gcgccccctt
     1861 tttcgccagg agctgcagga tgtcgagggc cggcgactcg cgcacgtcgc tgatgtcctt
     1921 cttgtacgcc acgcccagca ccagcacctt cgccccg
//
LOCUS       DASVJL010000101         4427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1047506, whole genome shotgun sequence.
ACCESSION   DASVJL010000101 DASVJL010000000
VERSION     DASVJL010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047506"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1662)
                     /locus_tag="VF357_01900"
     CDS             complement(184..1662)
                     /locus_tag="VF357_01900"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX7522927.1"
                     /translation="MFLGNLASLRYFLPEFAVTATILLLVVLHVVTKSPRSTASGFLS
                     LLGVGAAIFLAGITPPGAGRSIFEGMVAHDGFAVFFKVLTAVATLVVIFMSMDSKDLG
                     GRSQAEYYIFLLSTLLGMFLLSSATDIVMLYLSLELVSIPSYLLAGYLKGQEHSTEAA
                     MKYVVYGATASGVMIYGFSILFGLTGSTQIAEIGRSLAAGKATLPMLLASVMVAVGFG
                     YKIAAVPFHMWSPDVYEGAPTPATAFFSIGPKAAGFAVLVRFYYTVFAAPDPGTGLWR
                     LTSAVDWPFLFAALSALTMTVGNLVAIKQSNVKRLLAYSSIAHAGYMLMGVVLLSSAG
                     IEAILFYLVVYLFMNLGAFYVVVVVSNATRGEEISDFTGLGSRAPFAAVALSIFLFAL
                     TGIPPFSGFIGKVYLFAEVIHRGVYWLAVVAALNSVVSLYYYARIVRAMFLQEPTDAS
                     TIPVPAVSFAMLVLLAAPTLILGVYWEPVARIATDSVRMLAF"
     gene            complement(1664..3193)
                     /locus_tag="VF357_01905"
     CDS             complement(1664..3193)
                     /locus_tag="VF357_01905"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX7522928.1"
                     /translation="MGFVDSHILSLMTFLPILGAAVILCVPKGKDDVVRWIAAAASFL
                     PLVLAVKLWFAYDRAMAGVNVASQYQFVEHYLWIPSINAEYFLGADGISMPLLLLTAL
                     VSFLALIGSFGITKKVKGYMALFLLLETGMMGVFVSLDFFLFYVFWEVMLLPMYFLIG
                     IWGGPRKEYAAIKFFLYTLFGSVLMLIALLALYFNTTNPETGGHTFNLLHYMAQGTHN
                     AWLKGFDVRVVVFLGLFIGFAIKVPLFPFHTWLPDAHVEAPTAISVILAGVLLKMGTY
                     GLMRISFPIFPDVTRWFVVPMAVIGVVNIVYGALCALAQSDLKKMVAYSSVSHMGFVM
                     LGMAALTPLGMAGASMQMFSHGLITAMLFFLVGVVYDRAHHRQIDGFGGLGAVVPIYT
                     GFISFAFFASLGLPGMSGFIAEQMVFLGSFEAFRSIVIVAAIGIVFVAAFHLWSLQRV
                     FLGPLNPKYATMEEINAREMFCLAPLGILVLIIGVWPMPMLNLLNASAVRLVDLVKAV
                     I"
     gene            complement(3213..>4427)
                     /locus_tag="VF357_01910"
     CDS             complement(3213..>4427)
                     /locus_tag="VF357_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX7522929.1"
                     /translation="YIGFITLFIAATIALTQNDIKKVLAYSTVSQLGFMIMGLGVGGY
                     TAGLAHLATHAAFKACLFLGSGSVIHALHTQDLREMGGLRKKMPITFVTFLIATLAIA
                     GVPGFSGFFSKDMILAAALEFGMKNPAHYVLFFGALFTAGMTAFYMFRLVIMAFLGEP
                     KDHHKFDHAHESPPNMWVPLVVLAIFSFSFWFKSPFVEKGWVQTLIAKPATVANIASP
                     GSAAAPPAGHATAISPAPHEAAPAEGGEHDAHIAHMAHSYAMYSSVAIGGLGIFLGFV
                     VYFFGWIDPARVANSVKPLHNFLLNKWYFDELYDKTVIGGSIAFAKFLAWFDLHVVDG
                     LVNLAAQLGVFVSFLVGKFDDYVVDGAVNGVASTTIGGGSVLRRLQTGKLYHYVFVLA
                     GGALVIFLIKAF"
BASE COUNT          959 a         1385 c         1468 g          615 t
ORIGIN      
        1 tcgtacaccc cgtacttgac ccggtcgtac cgcttcgggc ccagcgcctg ggagaggagg
       61 acgaacccca ccgacatcgc cagcgcgatg accagaagca cgagaaccgc gaagtacggg
      121 ttcgagaagt ccacggttgc gaaaaagccg accatgggga gccccctcac gcctttccgt
      181 ggatcagaac gccagcatcc ggacggaatc ggtggcgatc cgcgccaccg gctcccagta
      241 cacgccgagg atcagcgtcg gcgccgcgag caggacgagc atagcgaagg agaccgcggg
      301 aacggggatc gtcgacgcat cggtcggctc ctggaggaac atcgcccgga cgatccgcgc
      361 gtagtaatag agcgacacca cgctgttcag cgcggcgacg accgcgagcc agtatacccc
      421 gcggtggatg acctcggcga acaggtaaac cttcccgatg aagccggaga acggcgggat
      481 cccggtcaag gcgaagagga agatggagag cgcgaccgcc gcgaaggggg cgcggctgcc
      541 gagtcccgtg aagtcggaga tctcctcccc tcgggttgcg ttgctcacca cgaccacgac
      601 gtagaaggcg cccagattca tgaagaggta gaccacgagg tagaacagga tcgcttcgat
      661 ccccgcggaa gacaggagca cgacccccat cagcatgtat ccggcgtggg cgatcgagga
      721 ataggccagg agccgcttca cgttgctttg cttgatcgcg accaggttcc cgacggtcat
      781 cgtcagcgcc gacagcgcgg cgaacaggaa cggccagtcc accgcggagg tcaggcgcca
      841 caggcccgtg ccggggtccg gggcggcgaa caccgtgtag tagaagcgga cgaggacggc
      901 gaaccccgcc gccttcggcc cgatggagaa aaaagcggtc gccggcgtgg gcgccccctc
      961 gtacacgtcg gggctccaca tgtggaacgg caccgcggcg atcttgtacc cgaaccccac
     1021 ggccaccatc acggaggcca gcagcatggg cagcgtcgcc tttccggccg ccaggctccg
     1081 cccgatctcc gcgatctggg tggaaccggt cagcccgaaa aggatggaga acccgtagat
     1141 catcacgccc gacgccgtgg ccccgtacac cacatacttc atcgcggcct cggtggaatg
     1201 ctcctgcccc ttgaggtacc ccgccagcag gtacgagggg atggagacga gctccagcga
     1261 caggtacaac atcacgatgt cggtcgccga ggaaagcagg aacatcccga gcagggtgga
     1321 gagcaggaag atgtaatact ccgcctggct ccggccgcca aggtctttgc tgtccatcga
     1381 catgaagatc accacgaggg tggccacggc cgtcaggacc ttgaagaaga ccgcgaaccc
     1441 gtcgtgggcg accatcccct cgaagatcga ccggccggcc cccggcgggg tgatccccgc
     1501 gaggaagatc gccgccccga ccccgaggag cgacaggaac ccggacgctg tggacctggg
     1561 gctcttcgtg accacgtgga gcacgaccag cagaaggatc gtcgccgtga cggcgaactc
     1621 cggcaggaag taccgcaggc tggccaggtt gcccaggaac atctcagatt accgccttca
     1681 cgaggtcgac caggcgcacc gcggaggcgt tcagcaggtt cagcatcggc atcggccaga
     1741 cgccgatgat cagcacaagg atccccagag gcgcgaggca gaacatctcc cgggcgttga
     1801 tctcctccat cgtggcgtac ttcgggttca gcggtccgag gaaaaccctc tgcagcgacc
     1861 acaggtggaa agcggcgacg aagacgatgc cgatggccgc cacgatcacg atggaccgga
     1921 acgcctcgaa ggagccgagg aagaccatct gctcggcgat gaacccggac atgcccggca
     1981 ggccgagaga agcgaagaag gcgaaggaga tgaagccggt gtagatgggc accacggcgc
     2041 ccagcccccc gaagccgtcg atctgccggt ggtgcgcccg gtcgtagacc acgccgacca
     2101 ggaagaagag catggccgtg atcaggccgt gggaaaacat ctgcatcgac gcccccgcca
     2161 tcccgagggg ggtaagcgcc gccatgccga gcatgacgaa ccccatgtgg ctgacggagg
     2221 agtaggcgac catttttttg aggtcggact gggccagggc gcacagcgcg ccatagacga
     2281 tgttcaccac cccgatcacc gccatcggca cgacgaacca cctcgtcaca tcggggaaga
     2341 tcgggaagga gatccgcatg agcccgtacg tccccatttt cagcagaacg ccggcgagga
     2401 tgacggagat ggcggtgggc gcctcgacgt gggcgtcggg aagccaggtg tggaacggga
     2461 agagcggcac cttgatggcg aagccgatga agaggccgag gaagaccacg acacggacgt
     2521 cgaacccttt cagccaggcg ttgtgcgtcc cctgcgccat gtagtggagc aggttgaagg
     2581 tgtggccgcc cgtctcgggg ttcgtcgtgt tgaagtagag ggccagcagc gcgatgagca
     2641 tcagcacgga gccgaacagg gtgtagagga agaacttgat cgccgcgtac tccttccggg
     2701 ggccgcccca gatgccgatc aggaagtaca tcggcagcag catcacttcc cagaagacgt
     2761 agaagaggaa gaagtcgagg gagacgaaga cgcccatcat cccggtttcg agcaggagga
     2821 agagggccat gtaccccttg accttcttcg tgatcccgaa ggagccgatg agcgcgagga
     2881 acgacacgag ggccgtcagg aggagcagcg gcatcgagat cccgtccgcc ccgaggaagt
     2941 actccgcgtt gatcgacggg atccagaggt aatgctccac gaactggtac tggctggcca
     3001 cgttgacgcc cgccatggcc cggtcgtaag cgaaccagag cttgaccgcc aggaccagcg
     3061 ggaggaagga ggccgcggcg gcgatccacc ggaccacgtc gtccttcccc ttggggacgc
     3121 acaggatcac cgccgctccg aggatcggga gaaacgtcat gagcgacagg atgtgactat
     3181 cgacgaaacc cacgaaagac cacctccatt cttcagaaag ccttgatcag gaaaatgacg
     3241 agagccccgc cggccagcac gaacacgtag tggtacagct tcccggtctg cagccggcgc
     3301 aggaccgatc cgcctccgat cgtcgtgctc gccacgccgt tcacggcacc gtcgaccacg
     3361 taatcgtcga acttcccgac gaggaacgag acgaagaccc cgagctgcgc ggcgaggttc
     3421 accaggccgt ccaccacgtg caggtcgaac caggcgagga acttcgcgaa ggcgatcgag
     3481 ccgccgatca ccgtcttgtc gtacagttcg tcgaaatacc acttgttcag caggaagtta
     3541 tgaagcggct tcacgctgtt cgccacgcgg gccgggtcga tccagccgaa gaagtagacg
     3601 acgaagccga ggaagatgcc gagccccccg atcgctaccg aggagtacat cgcataggaa
     3661 tgcgccatgt gcgcgatgtg ggcgtcgtgt tctcccccct cggcgggggc ggcttcgtga
     3721 ggagccggtg aaatcgccgt ggcgtgtccc gcgggcggcg cggcggcgga cccgggcgag
     3781 gcgatgttcg cgaccgtcgc cggcttcgcg atgagcgtct ggacccaccc cttctcgacg
     3841 aacgggctct tgaaccagaa ggagaacgaa aagatcgcca gcaccacgag cggcacccac
     3901 atgttcgggg gcgactcgtg ggcgtggtcg aacttgtggt gatctttcgg ctcgcccagg
     3961 aaggccatga tcacgaggcg gaacatgtag aaggcggtca tcccggcggt gaacagcgcc
     4021 ccgaagaaga ggacgtagtg cgccgggttc ttcatcccga attcgagcgc cgccgcgagg
     4081 atcatgtcct tgctgaagaa tcccgagaaa cccggcaccc cggcgatcgc cagcgtggcg
     4141 atcaggaaag tgacgaaggt gatgggcatc ttcttccgga gtccgcccat ctcgcgcagg
     4201 tcctgggtgt ggagcgcgtg gatgaccgac cccgacccga gaaagaggca cgccttgaac
     4261 gcggcgtggg tggccagatg ggccagcccc gcggtgtagc cgcccacccc gagccccatg
     4321 atcatgaagc cgagctggga gacggtcgag tacgccagca ccttcttgat gtcgttctgc
     4381 gtcagggcga tcgtcgccgc gatgaacagg gtgatgaacc cgatgta
//
LOCUS       DASVJL010000102         4796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_390357, whole genome shotgun sequence.
ACCESSION   DASVJL010000102 DASVJL010000000
VERSION     DASVJL010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390357"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /locus_tag="VF357_01915"
     CDS             complement(<1..1767)
                     /locus_tag="VF357_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7522930.1"
                     /translation="MDSELLQAMDALRGRADLSIEELVELAGRWTPSVAAGQSRHKVT
                     EVPTGRTLRYYITMGLIDRPLGYEGTRAHYGFRHFLQMLSIKALQARYFPLRKVRSMI
                     TGRPDAELEGVLLRMEGDLREGATPEEKERIEADAETAVHIVVREKKRFGKSLPDTVG
                     RSETMRGQRVKNKALREKITYATDAAELIKNGYKVGMSGFTGSGYPKLVPQALAEHIL
                     REHSKGGKFRISVMTGASTSSELDGVLAMVDGIELRMPYNSDPIAREKINYGKMEYFD
                     FHLGAVAQYVQCGFFGEIDLAIIEVAGITEDGKLIPSTSVGNNQVFIDAAREVILEVN
                     NYHPLELEGMHDIYEPELPPNVKPINILKPSDRIGTPYLSCPAEKIKAIVETNHPDRT
                     TVFKEPDHDSKAISEHILDFLRHEVAKGRIPQNLLPLQSGVGNVANAVLYGLLDSEFE
                     HMTVYTEVIQDGMLALIDAGKVDFASSTAFSVSPDMVEKFNENIRAYKDKILLRPQSI
                     SNHVGVIKRLGVIAMNGCVEFDLYGNVNSTHVNGTRIINGIGGSGDFARNSFISIFTT
                     SSVAGRNGSVSCVVPMVSHVDHC"
     gene            complement(1870..2169)
                     /locus_tag="VF357_01920"
     CDS             complement(1870..2169)
                     /locus_tag="VF357_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX7522931.1"
                     /translation="MKRGEREVTWWVYMIACRGGKIYTGTAIDPEARYEAHRAGKGAA
                     FTRANPPVALLRSVAFESRSEACRAEAALKKLSRDGKRAWARGESSLSKKMFNIK"
     gene            2263..2820
                     /locus_tag="VF357_01925"
     CDS             2263..2820
                     /locus_tag="VF357_01925"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX7522932.1"
                     /translation="MTGTLAAAAVLASFSGLLAWLYNRLVRLRNLSRSSWSDIDVLLK
                     KRHDLVENLVETVKGYAGYEQATLLGVTEARARAVRAAGPADRGKEEAVLGERVSSLF
                     AVAEKYPVLKASGGYLLLQKQLVEIENGIEYARRYYNAVVRDYNTATETFPSNLVAPL
                     FGFRGAEYFQLDSPAERERTDAQLS"
     gene            2804..4606
                     /locus_tag="VF357_01930"
     CDS             2804..4606
                     /locus_tag="VF357_01930"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX7522933.1"
                     /translation="MPNCRETHGPRRSAGREGAARAPTSPYLLPFFLALLLLFLPTPS
                     LAEDFSITSIRSDIEVRDDSSLRITETIDTVFLRQRHGLYRDIPFRYTDELGKTSFTP
                     IRVVSVRDASGNRWKHKVERSGGFVRVRIGDPDKFVEGRQVYVIEYSVENAALPFPDH
                     DELYWNITGNGWDVPIGSVSAHVAVAAGDRSLELRTRCFTGPRGSREQACTASPFRNG
                     ATFGSTRAFGPREGMTIVLGWEKGVVRPASPWRTYAFRLNLSENWVFLVPLLTLAYML
                     VRWYRVGRDPSASGPRVVAYGPPEEGGRPLLPAEVGVLLDERLDPRDITASVVDLAVK
                     GQITIEERMTPGLLFDKTDYVLKKAKGPGAELPPFERLLMERLFREHGDEVSVSDLKQ
                     KFYRDIEDLKKSAFEELERMKLFGANPLAVTQKYQFAGFAVIFAGVGLAMFASKIGLA
                     EGSSPVVASILSGAVVFLFAPHMPVKTVNGVKAMGAILGFEEFLSRTERDRLERMKDA
                     NLFEKCLPYAIALGVSERWAKAFEGIQQEPPRWYVSHGGAGEFRPSAFHHSLDSALSS
                     MTAAMQSSPRSGGSGFSGGGGSGGGGGGGGGGSW"
BASE COUNT          800 a         1565 c         1537 g          894 t
ORIGIN      
        1 gcaatggtcc acgtgggaca ccatcggcac gacgcaggag acgctcccgt tcctccccgc
       61 caccgaggag gtggtgaaga tggagatgaa ggagttccgc gcgaagtcgc cggagccgcc
      121 gatgccgttg atgatccgcg tgccgttgac gtgggtggag ttcacgttcc cgtacaggtc
      181 gaactccacg caaccgttca tcgcgatcac gcccagccgc ttgatgactc ccacgtggtt
      241 gctgatggac tggggccgca ggaggatctt gtccttgtag gcgcggatgt tctcattgaa
      301 cttctccacc atgtccgggg agacggagaa ggccgtggag gaggcgaagt cgaccttccc
      361 ggcgtcgatg agggccagca ttccgtcctg gatgacctcg gtgtagacgg tcatgtgctc
      421 gaactcggag tcgagcagcc cgtacaggac cgcgttcgca acgttcccca cgcccgactg
      481 caggggcagc aggttctgcg ggatccgccc cttggccacc tcgtggcgca ggaaatccag
      541 gatgtgctcc gagatcgcct tgctgtcgtg gtccggctcc ttgaagacgg tcgtccggtc
      601 ggggtggttc gtctcgacga tcgccttgat cttctccgcc ggacaggaca ggtacggcgt
      661 gccgatccgg tcggacggct tgaggatatt gatgggcttc acgttgggcg gcagctcggg
      721 ttcgtagatg tcgtgcatcc cctcgagttc gagcggatgg tagttgttca cctcgagaat
      781 gacttcccgc gccgcgtcga tgaagacctg gttgtttccc acggaagtgg aggggatcag
      841 cttcccgtcc tcggtgatcc cggcgacctc gatgatggcc aggtcgatct cgccgaaaaa
      901 gccgcactgg acgtactgtg ccaccgcccc gaggtgaaag tcgaagtact ccatcttccc
      961 gtaattgatc ttctcccgcg cgatcgggtc ggagttgtac ggcatccgca gctcgatccc
     1021 gtccaccatc gccagcaccc cgtcaagctc gctgctggtg gaggcccccg tcatgacgga
     1081 aatgcggaac ttccccccct tgctgtgctc ccgcaggatg tgctcggcga gcgcctgggg
     1141 gacgagcttg gggtaccccg agcccgtgaa gccgctcatc ccgaccttgt agccgttctt
     1201 gatcaactcc gccgcgtccg tggcgtaggt gatcttctcc cgcaacgcct tgttcttgac
     1261 ccgctgtcct cgcatcgttt cgctcctccc cacggtgtcg ggaagggact tcccgaaccg
     1321 tttcttctcc ctgacgacga tatggacggc cgtctcggcg tccgcctcga tccgttcctt
     1381 ctcctccgga gtcgcaccct cccgcaggtc cccctccatc cgaaggagta cgccctccag
     1441 ttcggcatcg ggtcgccccg tgatcatgga gcggaccttc cgcaagggaa agtaccgcgc
     1501 ctggagcgcc ttgatggaga gcatctggag gaagtggcga aacccgtaat gggcgcgcgt
     1561 accctcgtac ccgagaggac ggtcgatcag ccccatggtg atgtagtacc ggagggtcct
     1621 cccggtgggg acttctgtca ccttgtgacg cgactggccc gccgcgacgg agggagtcca
     1681 tcggccggcc agttcgacca gttcctcgat ggagaggtcg gcccggcccc gcaacgcgtc
     1741 catggcctgg aggagttcgc tgtccatgaa aagcgaattt actgtaaaca ctatataatg
     1801 tcaacagtaa acatcccgca acccaacagc gcaagccggc gcaataaaaa taaacgtttt
     1861 tggaaataac tatttaatat taaacatttt tttacttaag gacgactcgc ccctggccca
     1921 ggcccgtttt ccatcccggg acaatttttt cagcgccgcc tccgcccggc acgcttccga
     1981 ccggctttcg aaggcgacgg accggagcag cgccaccggc gggtttgccc gggtgaacgc
     2041 cgcccctttc ccggcgcgat gcgcctcgta ccgcgcctcg gggtcgatcg ccgtcccggt
     2101 gtagatcttc cccccgcgac acgcgatcat gtacacccac caggtgactt cccgctcccc
     2161 ccgcttcacg gctttatctt cgcacccgcc gggccggaca tatattaaaa tcatgctacc
     2221 cggatccggg acggaaccgg acggaagggg acggagccga caatgaccgg cacgctggca
     2281 gcggcagcgg ttctcgcctc gttctccggc ctcctggcgt ggctctacaa ccggctcgtc
     2341 cggctgcgga atctttcacg ctcctcctgg tcggacatcg acgtcctcct gaagaagcgg
     2401 cacgacctgg tggaaaacct ggtcgaaacc gtgaaggggt acgcagggta cgaacaggcc
     2461 accctgctgg gggtcaccga ggcccgtgcc cgggcggtcc gggccgcggg gccggccgat
     2521 cgggggaagg aggaggccgt tctcggggag cgggtttcgt ccctgttcgc cgtggcggaa
     2581 aagtacccgg tgctcaaggc cagcggaggg tacctcctgc ttcagaagca gctcgtcgag
     2641 atagagaacg gtatcgagta cgcccgccgg tactacaacg ccgtcgtccg ggactacaac
     2701 accgcgacgg agacgttccc ctccaacctg gtggcccccc tgttcggctt ccggggagcg
     2761 gagtacttcc agctcgactc tccggcggag cgcgagagga ccgatgccca attgtcgtga
     2821 gacacacggt ccgcgacggt cggcgggtcg ggagggggcc gcccgggcgc cgacctcccc
     2881 gtacctcctg ccgtttttcc tggcgctgct gctcctgttc ctccccacgc catccctggc
     2941 ggaagatttt tcgatcacgt ccatccggtc cgacatcgaa gtccgggacg actcctccct
     3001 ccggatcacc gagaccatcg acacggtctt cctccggcag cgacacgggc tctaccggga
     3061 catcccgttc cggtacaccg acgaactcgg gaagacttcc ttcacgccga tccgggttgt
     3121 ttcggtgagg gacgcgtccg gcaaccggtg gaagcacaag gtggaacgga gcggggggtt
     3181 cgtcagggtc cggatcgggg atcccgacaa gttcgtggag ggacggcagg tgtacgtcat
     3241 cgaatactcg gtggagaacg cggccctccc ctttccggac cacgacgagc tctactggaa
     3301 catcacgggg aacgggtggg acgtcccgat cgggtcggtc tccgcccacg tcgccgtcgc
     3361 ggccggagac cgctccctgg agctgcgtac ccgctgcttc accggtcccc ggggctcccg
     3421 cgagcaggcg tgcacggctt cccccttccg caacggggcg accttcggtt cgacccgcgc
     3481 gttcgggccc cgggaaggga tgaccatcgt cctcgggtgg gaaaaaggtg tcgtgcggcc
     3541 cgcttccccc tggaggacgt acgccttccg tctcaacctc tccgagaact gggtgttcct
     3601 tgtccctctt ctcacactcg cctacatgct ggtccgctgg taccgggtag ggagggaccc
     3661 gtccgcgagc ggcccgcggg tcgtcgcgta cggtccgccg gaggagggcg gtcgccctct
     3721 cctgcccgcc gaggtcggag tgctcctcga tgagcggctc gaccccaggg acattaccgc
     3781 atccgttgtg gacctcgccg tgaaggggca gatcacgatc gaggagagga tgaccccggg
     3841 gctcctgttc gacaagaccg actacgtcct gaagaaagcg aaggggccgg gcgcggagct
     3901 tccacccttc gagcggctgc tgatggagag gctcttccgg gagcacgggg acgaggtgag
     3961 cgtctccgac ctcaagcaga agttctaccg ggacatcgag gacctgaaga agtccgcgtt
     4021 cgaggagttg gagcgtatga agctcttcgg ggcgaacccg ctggcggtga cgcagaagta
     4081 ccagttcgcg gggttcgccg ttatcttcgc gggggtcgga ctcgcgatgt tcgcctcgaa
     4141 gatcggcctg gcggagggct cctccccggt cgtcgcctcc atcctctccg gggccgtcgt
     4201 gttcctcttc gccccccaca tgccggtcaa gaccgtgaat ggggtgaagg cgatgggggc
     4261 gatccttgga ttcgaggagt tcctctcaag gacggagagg gaccggctgg agcggatgaa
     4321 ggacgccaac ctgttcgaga agtgcctgcc gtacgcgatc gcgctggggg tctccgagcg
     4381 gtgggcgaag gcgttcgagg ggattcagca ggagcctccc cgctggtacg tttcccacgg
     4441 cggggccggc gagttccggc cgagcgcctt ccaccactcc ctggattccg ccctctcctc
     4501 aatgaccgcg gcgatgcaat cctctccccg cagcggcggg agcggctttt cgggaggagg
     4561 gggatccggg ggcggagggg ggggcggagg cggggggagc tggtagacca ccggcgccgc
     4621 cgaaggaaga cccgaacgga aattccgcgg cagaaaattg tccgggtggc gtcgtccccc
     4681 gtagtccctc ctccttgacg tttcgtcccc gcgggactat cttctggatg tttcccaccc
     4741 ccctcatccc atcttcggaa ggagccgccc gatgccggaa ttctcgtacc aggacc
//
LOCUS       DASVJL010000103         3749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_860046, whole genome shotgun sequence.
ACCESSION   DASVJL010000103 DASVJL010000000
VERSION     DASVJL010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860046"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VF357_01935"
     CDS             complement(<1..1052)
                     /locus_tag="VF357_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX7522934.1"
                     /translation="MHPDSFGTFSSKTVGGHAFGIFRLEALEKRHVGKVSRLPFSIKV
                     LLENLLRHEDGATVTADDIRALANWSPKEPSDREIAFRPARVLLQDFTGVPALVDLAA
                     MRDAARRMGGDPKRINPLMPADLVIDHSVQVDRFAAVTAFPDNVAREYGRNGERYAFL
                     RWGKESFRNFRVVPPGTGICHQVNLEHLAPVVFTRKDRSGALAYPDTLVGTDSHTPMI
                     NGLGVVGWGVGGIEAEAAMLGQPISMLIPEVVGFRMTGKLPVGATATDLVLTVVQMLR
                     KKGVVGKFVEFYGKGLSSLSVADRATISNMSPEYGATIGFFPVDRETLAYLLFTGRDR
                     EQVKLVEAYCKAQGLFR"
     gene            1129..1689
                     /locus_tag="VF357_01940"
     CDS             1129..1689
                     /locus_tag="VF357_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEX7522935.1"
                     /translation="MTLLGIAVGLAMDAFAVAIATGITLGAVSVRQTFRLAFHFGLFQ
                     FLMPVIGYLAGMTVERYIKGYDSWLAFVLLGCIGGKMVYDGSWGDGEEGNGGKDPTRG
                     ISLIMLSVATSIDALAVGLSLGVLRNEGIVYPGIVIGIVACTFTAAGLHLGKRLGTVF
                     GKRMEVVGGLVLVAIGVKILFDHYRG"
     gene            1713..2405
                     /locus_tag="VF357_01945"
     CDS             1713..2405
                     /locus_tag="VF357_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX7522936.1"
                     /translation="MNGEWLDILTHLLVATIAGGLIGLERSYHGRPAGFRTHTLVCVA
                     SSLLMLVTMYQAKWFTGAPPDSVRIDPTRMAQGVMTGIGFLGAGVIMREGLTVRGLTT
                     AASIWVTAAIGILAGVGFYSAVLVGSVITIGILTVFRKVENRMPSQIFANTTITFGRD
                     NYMSETEIRELHLKNGFSVANVSYRLMEKGKQLEYRMTIRTMDGGNIEKLSKTLLGLT
                     SIVEFNISMAGD"
     gene            2465..>3749
                     /locus_tag="VF357_01950"
     CDS             2465..>3749
                     /locus_tag="VF357_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522937.1"
                     /translation="MENNALAMRAINTIRFLSVDAVQKAKSGHPGTPMGLAPLSYLLW
                     TNYLRYNPANPDWAGRDRFVLSCGHASLLLYSLLHLTGFDVTLDDLREFRQWGSRTPG
                     HPEARHTPGVEVTTGPLGQGLGNAVGMAMASRMMAQQFNRPGHEIVSHRIVALCSDGD
                     LMEGIASEAASLAGFHRLGNLVAFYDDNRITIEGSTDLAFREDTAGRFRSYGWNVLNV
                     ADGNTDLAGISAAIEVAFSERERPTLVIVRTSIGYGSPNKQDTAGAHGAPLGEAEVVL
                     AKEHLGWPVTPAFLVPDDVLAHFREALARGEKAEKEWRMKFAAYAAAFPALALEWERR
                     MWGDLPEGWADGIPTFSPEAGAIATRSASGKVLNAIAAKVPELVGGSADLSPSTETVI
                     KGGGEFLPDAGPGGRNFHFGVREHGMGAVLNGMARH"
BASE COUNT          652 a         1128 c         1277 g          692 t
ORIGIN      
        1 cggaagagcc cctgcgcctt gcagtacgcc tccacgagct tcacctgctc cctgtcccgg
       61 ccggtgaaga gaaggtaggc gagcgtctcc cggtcgacgg ggaagaagcc gatcgtcgcg
      121 ccgtactccg gcgacatgtt cgagatcgtc gcccggtcgg ccacggagag ggaggagagc
      181 ccctttccgt agaactcgac gaactttccc accacgcctt tcttccgcag catctggacg
      241 acggtgagga cgaggtcggt cgcggtcgcc ccgacgggga gcttcccggt catccggaac
      301 cccaccacct cgggaatgag catcgagatc ggctggccga gcatcgccgc ctcggcttcg
      361 atcccgccca cgccccaccc caccacgccc agtccgttga tcatcggcgt gtgggaatcg
      421 gtccccacga gggtgtccgg gtacgccaac gcgccggagc ggtctttccg cgtgaagacg
      481 acgggggcga ggtgttccag attcacctgg tggcagatcc cggtgccggg cgggacgacg
      541 cggaagttgc ggaacgactc cttcccccac cgcaggaacg cgtatcgctc cccgttgcgc
      601 ccgtactccc tcgccacgtt gtcggggaag gcggtcaccg cggcgaaccg gtccacctgc
      661 acggagtggt cgatcacgag gtccgccggc atcagcgggt tgatccgctt cgggtccccg
      721 cccatccgcc tcgcggcgtc gcgcatcgcc gccagatcga ccagcgcggg gacgccggtg
      781 aaatcctgca ggagcacgcg cgccgggcgg aaggcgatct cgcggtccga cggctctttc
      841 ggcgaccagt tcgcgagcgc ccggatgtcg tccgccgtga ccgtggcgcc gtcctcgtgg
      901 cgcaggagat tttcgaggag gaccttgatg gagaagggga ggcgggagac cttgccgacg
      961 tggcgcttct cgagcgcctc caggcgaaag atcccgaagg cgtgtccgcc gacggtcttg
     1021 ctcgaaaagg ttccgaaact gtccggatgc atggcgtcac gacctcccac gcggtatctt
     1081 tgaccgaatg ctttatggta gcactccggg gaggttccga tcgacacctt gacgcttctc
     1141 ggcatcgcgg tggggctggc catggacgcc ttcgccgtgg cgatcgccac ggggatcacg
     1201 ctgggggcgg tctccgttcg ccagaccttt cgcctcgcct tccacttcgg cctcttccag
     1261 ttcctgatgc cggtgatcgg gtaccttgcg ggaatgaccg tggagcgtta catcaagggg
     1321 tacgactcct ggctcgcctt cgttctgctc ggctgcatcg gcgggaaaat ggtgtatgac
     1381 gggagttggg gagacgggga ggaggggaac ggcgggaagg accccacgcg cgggatatcc
     1441 ctgatcatgc tgtcggtggc gaccagcatc gatgcgctgg cggtgggact cagcctcggc
     1501 gtgcttcgca acgaggggat cgtctacccc gggatcgtga tcgggatcgt ggcgtgcacc
     1561 ttcaccgccg cggggctcca cctcggcaag cgcctgggca ccgtcttcgg caagcggatg
     1621 gaagtcgtcg ggggactcgt cctcgtcgcc atcggagtga agatcctgtt cgaccattac
     1681 cgggggtgac gccacggaaa ggagttcatg gcatgaatgg agaatggctc gatatcctca
     1741 cgcatctgct tgttgcgaca attgcaggtg ggctgatcgg gttggaacgg agttatcatg
     1801 gccgcccggc gggattcagg acacatacgt tggtttgtgt cgcatcaagc ctgctgatgc
     1861 tcgtcacgat gtaccaagcc aagtggttta ccggagcgcc gcccgattcc gttcgaatag
     1921 acccgacgcg aatggcacag ggggtcatga ccgggatagg ctttcttggt gcgggcgtta
     1981 tcatgcggga aggacttacc gtacgggggt tgacgactgc ggcttccatc tgggttacgg
     2041 ctgcaatagg tattcttgcg ggggtaggtt tctacagcgc cgtcctcgtt ggatcggtca
     2101 tcaccatcgg gatattgact gttttccgaa aggtggaaaa caggatgcct tcgcagatat
     2161 ttgcaaatac tacgatcacc ttcggtcggg acaattatat gtccgagacg gaaatcaggg
     2221 aattgcattt aaaaaacggc ttttcggttg ccaatgtaag ctacagattg atggaaaagg
     2281 gtaagcagtt ggaatatcgc atgaccatac gtaccatgga tggcggcaat attgaaaaat
     2341 tgtcgaaaac tctcctgggt ttgacctcga ttgtcgaatt caatatttcc atggcagggg
     2401 attaagcgga ggggatcggc gatggtaaga tgaatcttcg ctgatgcaat caatggaggc
     2461 caccgtggag aacaacgcgc tcgcgatgcg ggcgatcaac accatccggt tcctgtcggt
     2521 cgacgcggtc cagaaggcga agtcggggca tcccggcacg ccgatgggac tggcgccgct
     2581 gtcgtacctg ctgtggacga attatctgcg atacaaccct gcgaaccccg attgggccgg
     2641 gcgcgaccgt ttcgtcctct cctgcggcca cgcctccctg ctcctctact ccctgctcca
     2701 cctcacgggg ttcgacgtga ccctcgacga ccttcgggag ttccggcagt ggggaagcag
     2761 gacccccggg catcccgagg cgaggcacac cccgggcgtc gaggtgacca cgggaccgct
     2821 cgggcaggga ttggggaacg ccgtcgggat ggcgatggcc tcccggatga tggcgcagca
     2881 gttcaaccgc cccgggcacg aaatcgtctc gcatcggatc gtggcgctgt gctccgacgg
     2941 cgacctgatg gaggggattg cctccgaggc ggcgtctctg gccggattcc accggcttgg
     3001 gaacctcgtg gcgttctacg acgacaaccg gatcacgatc gaagggtcca cggacctcgc
     3061 cttccgggag gacacggcgg gtcgcttccg ttcgtacggg tggaacgtgc tgaacgtcgc
     3121 tgacgggaac acggatcttg ccgggatttc ggcggcgatc gaggtggcct tcagtgagag
     3181 ggagcgtccc accctggtga tcgttcggac gagcatcggg tacggcagcc ccaacaagca
     3241 ggacaccgcg ggcgcgcacg gggcgccgct gggggaagcc gaggtcgtcc tcgcgaagga
     3301 acacctcggg tggcccgtca ccccggcctt cctcgtgccc gacgacgttc tcgcccattt
     3361 ccgggaggcg ctggcgcgcg gggagaaggc ggaaaaggag tggcggatga agttcgccgc
     3421 gtacgccgcg gcgttcccgg cgctcgccct cgaatgggag cggcggatgt ggggcgatct
     3481 ccccgagggg tgggcggacg ggatcccgac gttttcgccc gaggccggcg cgatcgccac
     3541 ccggagcgcg tccggcaagg tgctgaacgc gatcgccgcg aaggttcccg agcttgtcgg
     3601 agggtcggcg gacctttccc cttcgacgga gacggtgata aagggcggcg gcgagtttct
     3661 tcccgatgcc gggccgggcg ggcggaattt ccatttcggg gtgcgcgagc acgggatggg
     3721 ggcggtcctt aacggtatgg cgcggcacg
//
LOCUS       DASVJL010000104         1717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_953810, whole genome shotgun sequence.
ACCESSION   DASVJL010000104 DASVJL010000000
VERSION     DASVJL010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953810"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VF357_01955"
     CDS             <1..211
                     /locus_tag="VF357_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522938.1"
                     /translation="QKTKAFVALKPGVTPSEALKKELIEHCKGKIAVYKLPREIEFID
                     AMPRTAVGKLLRRILRQQEIEKQKK"
     gene            complement(301..1422)
                     /locus_tag="VF357_01960"
     CDS             complement(301..1422)
                     /locus_tag="VF357_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7522939.1"
                     /translation="MEETGQAAKEERRPGPDRSPLRTGSTFLSILGLIALLHFAASVF
                     ITLFSAMLLAFALDPLVHLLCVRTRLRRHHASGIVVFLFVAMLYGLFYGTYLRAESFL
                     AEIPAIAEKIRSAPMVVSLANRAEEMDRIFAEAGRRITPSAGASTRPKTTPPVMVRDA
                     ESLTGTLLQGLGSLGGILFALGFIPFLVYFILADREPLTRRTRELFPEEHRATVGEIL
                     LDIERMMRNFLLGNALIALILSAATVLVFLLVGLPYAVVLGILSGSLSIVPYLGLPLA
                     LLPGVVVGLVSFESGGPFLILIAVVTMLHLVAANYLTPKLVGRGVRLNATASTVALLF
                     FGWLWGGMGLLLGIPILAVLKCITDNIPSTRRFGMFLGE"
BASE COUNT          340 a          538 c          598 g          241 t
ORIGIN      
        1 gcagaagacc aaggcgttcg tcgcgctgaa gcccggcgtc acgccgtccg aggcgctgaa
       61 gaaggagctc atcgagcatt gcaaggggaa gatcgccgtc tacaagctgc cgcgggagat
      121 cgagttcatc gacgcgatgc cgcggaccgc ggtcggcaag ctccttcgcc gcatcctgcg
      181 ccagcaggaa atcgagaagc agaagaagta aggcagggga ccgccgcccc ggccggcgcg
      241 atggcgacga gagagcccgg ggacgtgacg cccccgggct ttttttttaa acgggccccg
      301 ttattccccg aggaacatcc cgaaccgccg cgtggacggg atgttgtcgg tgatgcattt
      361 caggacggcc aggatcggga tccccagcaa cagccccatc cccccccaca gccagccgaa
      421 gaagagcagg gccaccgtcg aggcggtcgc gttcagccgc accccccggc ccaccaactt
      481 cggcgtcagg tagttcgccg ccacaaggtg aagcatggtc accacggcga tcaggatcag
      541 aaagggcccc cccgactcga aggagacgag gccgacgacg acgccgggaa gcagcgcgag
      601 cggcaggccg aggtacggga cgatgctcag cgacccgctc aggatcccga gaacgacggc
      661 gtacggaagg cctacgagca ggaagaccag aacggtcgcc gcggagagga tgagggcgat
      721 cagggcgttt ccgaggagga aatttcgcat catccgctcg atgtcgagga ggatctcccc
      781 caccgtcgcg cggtgctcct ccgggaagag ctcccgcgtc cgacgggtga gcggttcccg
      841 gtccgcgagg atgaagtaga cgaggaacgg gatgaagccg agcgcgaaga ggatgccgcc
      901 cagggagccg agcccctgga gcagcgtccc cgtgagcgat tccgcatccc gcaccatgac
      961 cggcggggtg gttttcgggc gcgtggaagc ccccgccgag ggagtgatac ggcggcccgc
     1021 ctcggcgaag atgcggtcca tctcttcggc cctgttcgcg aggctcacga ccatcggcgc
     1081 agagcggatc ttttcggcga tcgcggggat ctccgcgagg aagctttccg cgcgcaggta
     1141 cgtgccgtag aaaagcccgt agagcatcgc gacgaacagg aagaccacga tgccgctggc
     1201 gtgatggcgc cgcaagcggg tccgcacgca gagcaggtgg accagcggat cgagggcgaa
     1261 ggccagcagc atggcggaga acagggtgat gaagacggag gcggcgaagt gcaggagcgc
     1321 gatgaggccg aggatcgaaa ggaaagtcga acccgtccgg aggggactcc ggtccggccc
     1381 cggtcgccgc tcctccttcg cggcctgccc cgtttcctcc atcgcggctt gcgcttcttc
     1441 ggaggacaag ccggaggaca agtcggaggg caagccggag ggcgggtcgg agggcaggcc
     1501 ggagggcaag tcctgtttcg tgccttgcgt cgaggtcatt tgatcacttt accgtttttc
     1561 cgattcctcg cctacacccg tttgacaacc ggtcagggag aagcgaccct gacggggaga
     1621 tggcgccttc cctttcgcac acgatcgaga tccgcgtccg gttcggcgag accgacccgt
     1681 acggcgtggc gtacttcgcc gcgatcctcg actactt
//
LOCUS       DASVJL010000105         4329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_766360, whole genome shotgun sequence.
ACCESSION   DASVJL010000105 DASVJL010000000
VERSION     DASVJL010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766360"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1280
                     /gene="hflX"
                     /locus_tag="VF357_01965"
     CDS             156..1280
                     /gene="hflX"
                     /locus_tag="VF357_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX7522940.1"
                     /translation="MIRESRTERALLVYAHRKRVRGPAAVQHVSEVMDELKDLVLAAG
                     ASVVASRVQSVDSENPATVVGKGTLLRLKQEIETLGANLVVFQNLLKPKQQALLEEEL
                     EVKTLDRREVILDIFARRARTREGKLQVELAQLSFRLGRLAGGRKELSRLGGGIGTRG
                     PGEKKLEEDRRRIRAQIRQLERELNTVRRTRSLHYQRRREIGFPVVALVGYTNAGKST
                     LFNRLTGADVFVADQLFATLDPTARKFRLPGGREAILVDTVGFIHDLPAELRQAFLAT
                     LEGIGEADLLLHVADGSSDAMESNVGSVNAILGELSFREKPIVLLMNKRDRCLPGTPP
                     QDGALWISAKSGEGIPELLTLIERELWFHRPQENASTPPD"
     gene            1235..1837
                     /locus_tag="VF357_01970"
     CDS             1235..1837
                     /locus_tag="VF357_01970"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX7522941.1"
                     /translation="MVPPSARERFDAARLINIANGLTAARVLLVPYFAYLLISERGKA
                     ALLVFAVCGATDALDGLLARWLRQRTLAGALLDPIADKLLMATAFIVLSYVHIAPLRL
                     AIMVISRDIFILVGSFLYLLLFDSSDIRPTGLSKANTAVQILTVIYFLSVAAFPAEAK
                     ALGTGRGSLPDRAVTLICAVTTVASGLQYLYLGIRKLSDA"
     gene            1830..3170
                     /locus_tag="VF357_01975"
     CDS             1830..3170
                     /locus_tag="VF357_01975"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX7522942.1"
                     /translation="MREGAGEGSSRSGVGVEAVTPGSPADRAGIVAGDRILSVSGHSV
                     EDLLDLHFFTSRSRFTLAWRDASGAGRKKEFRPKGETPGIFPEPIRVRRCRNRCIFCF
                     VHQLPKGLRRTLYVKDEDVRLSFLHGQYVTFSDLSEEEAAKIVRYRLSPLYVSIHTTD
                     PELRRRMLGNPRAADVMRVMRRLIRAGIALHGQIVVCPGVNDGAELVRSLRDLSGLRP
                     GLRTVAVVPVGLTSHRAGLPPLRPVTRYQAGETLDLIRALGREFGKGADGEPFAVAAD
                     EYYLMAGRDVPGRRSYGSFAQIENGVGLVRRFRDEASSLFRRKRWTGGATGGTVATGR
                     SASRLVAGFLQEFSTRARARFVAAPVVNHLMGNSVTVTGLLGGNDIAEAVRGHVRGTL
                     YIPSVALRDAGDLFLDGLSPEEVSRRTGAPVVLFEPTPRGFHDAVYPRNRPEYH"
     gene            3218..3294
                     /locus_tag="VF357_01980"
     tRNA            3218..3294
                     /locus_tag="VF357_01980"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3252..3254,aa:Val,seq:gac)
     gene            3400..>4329
                     /locus_tag="VF357_01985"
     CDS             3400..>4329
                     /locus_tag="VF357_01985"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX7522943.1"
                     /translation="MTLLELARKEGKAKVAIAARVDGVVMDLARPLSDGAKVEWVLPA
                     DPDGVEILRHSTAHVMAAAVKELFPRALITIGPAIENGFYYDFDVETPFTPEDLVRIE
                     DRMREIVKADHPFVREEATKEQARVLFPGEPYKEELLAAIPDETVSLYRMGNFLDLCR
                     GPHVPGTGRVGAFHLMNTAGAYWRGDSKNKMLTRIYGIAFASKKELDEHLRILEEIKK
                     RDHRKIGRELDLFSVTDDIGPGLILWHPKGSVVRRVMEDFWRDEHTKAGYDLVFSPHI
                     ARLDLWRISGHTDFYRQAMFSPIDIEGQEYQLKP"
BASE COUNT          724 a         1383 c         1476 g          746 t
ORIGIN      
        1 aacgccggag agaagaaggg gttcctccac gcgatccgtc cgggagagtt cgatgcggtg
       61 atcgccctgc ccgacggttg cggtgtggcg ccgtcgctgg agctggcgcg ggaccttcgc
      121 ggccgcttcg gctacgacgt gctgcgtctc cacggatgat ccgggaatcg cgcacggaga
      181 gggctctcct tgtctacgcc caccggaagc gggtgcgtgg cccggcggcg gtgcagcacg
      241 tctccgaggt gatggacgaa ctgaaagacc tggtcctcgc ggcgggggcg agcgtcgtgg
      301 cgtctcgcgt gcaaagcgtc gactcggaga acccggccac ggtcgtcgga aaggggaccc
      361 tcctccgcct gaagcaggag atcgaaacgc tcggggcgaa cctggtggtc ttccagaacc
      421 tgctcaaacc gaaacagcag gcgcttctcg aagaggagct cgaggtgaag acccttgacc
      481 gccgcgaggt catcctcgac atcttcgccc ggcgcgcgcg cacccgggag gggaagctcc
      541 aggtggaact tgcccaactg tcgttccggc tcgggaggct cgcggggggg cggaaagagt
      601 tgtcgcggct cggcggcggg atcggcaccc ggggaccggg ggagaagaag ctcgaggagg
      661 atcgtcgccg catccgcgca cagatccggc agctcgagcg ggagttgaac acggtgcgca
      721 ggacgcgttc gctccattat cagcggcgcc gggaaatcgg gttccccgta gtcgcgctcg
      781 tcgggtacac caatgcgggg aaatcgaccc tcttcaatcg gctgacgggg gccgacgtct
      841 tcgtggcgga ccagctcttc gccacgctcg acccgaccgc ccggaagttc cggcttcccg
      901 gcggcaggga ggcgatcctc gtcgacacgg tggggttcat ccacgatctt ccggccgaac
      961 tgcggcaggc gttcctcgcg accctcgagg ggatcgggga agcggacctt ctcctccatg
     1021 tcgccgatgg aagctccgac gcgatggaga gcaacgtcgg atcggtgaac gcgatcctcg
     1081 gggaattgtc gtttcgcgag aagccgatcg tcctcctgat gaacaagcgc gaccgctgcc
     1141 tcccggggac gccgcctcag gatggggcgc tgtggatttc cgcgaagtcg ggggagggga
     1201 tcccggaact actcacgctg attgaaaggg aactatggtt ccaccgtccg caagagaacg
     1261 cttcgacgcc gcccgactga tcaacatcgc caacgggctg acggcggcgc gggtcctcct
     1321 ggtcccgtat ttcgcctacc ttctgatctc cgagcggggg aaggcggcgc tgctggtgtt
     1381 cgccgtctgc ggggcgacgg acgcgctcga cggtctgctg gcccgctggc tgcggcaacg
     1441 gacgctggcc ggggcgctcc tcgacccgat cgccgacaag ctgctgatgg cgaccgcctt
     1501 catcgtcctc tcctacgtcc acatcgcccc cctgcggctc gccatcatgg tgatcagcag
     1561 ggacatcttc atcctcgtgg ggagcttcct ctacctcctc ctgttcgatt cgagcgacat
     1621 ccggccgacg gggctcagca aggcgaacac ggcggtccag atcctgacgg tcatctactt
     1681 cctctcggtc gcggcgtttc ccgcggaggc aaaggcgctg ggaacgggaa ggggcagcct
     1741 tcccgaccgg gccgtcacgc tgatctgcgc ggtcacgacg gtcgcctccg ggctccagta
     1801 cctgtacctc ggcatccgga agctctccga tgcgtgaggg agccggagaa ggttcgtcgc
     1861 ggagcggcgt cggcgtcgaa gcggtgacgc ccggatctcc cgcggaccgg gccggtatcg
     1921 tcgccgggga ccggatcctc tccgtgtcgg gccattcggt cgaggacctc ctcgatctcc
     1981 atttcttcac ctcgaggagc cggtttaccc tcgcgtggcg ggacgcgtct ggggccggcc
     2041 ggaaaaagga gtttcgcccg aagggagaga cgccgggaat atttccggag ccgatccgcg
     2101 tacgtcgctg ccgcaaccga tgcatcttct gtttcgtcca ccagctgccg aaggggcttc
     2161 gccgcacctt gtacgtgaag gacgaggacg tccgcctctc ctttcttcac ggacaatacg
     2221 tcaccttttc ggatctttcc gaagaggagg cggcgaagat cgtccggtac cgtctctcgc
     2281 cgctctacgt gtcgatccac acgaccgacc cggaactgcg ccggcggatg ctcgggaacc
     2341 cgcgggcggc cgacgtaatg cgcgtgatgc ggcggctgat ccgcgccggg atcgcccttc
     2401 acggccagat cgtcgtctgc cccggcgtca acgacggcgc ggagctcgtg cgaagcctcc
     2461 gggacctctc cggcctgcga cccgggctcc gcacggtggc ggtggtcccg gtgggactca
     2521 cttcccatcg ggccggcctg ccgccgctgc gcccggtcac gaggtaccag gcgggggaga
     2581 cgctcgatct gatccgcgcg ctgggcaggg aattcgggaa aggggctgac ggggaaccgt
     2641 tcgcggtcgc cgccgacgaa tattacctga tggcgggtag ggacgttccc ggccgccggt
     2701 cgtacggatc gttcgcgcag atcgaaaacg gcgtggggct ggtccgtcgg ttccgggacg
     2761 aagcgtcctc tctgttccgg aggaaacggt ggacgggggg cgccaccggg ggtaccgtcg
     2821 cgactggccg gtccgcgtcg cgccttgtgg ccggttttct tcaggaattc tccacccgcg
     2881 cgagggcgcg gttcgtcgcg gcgccggtcg tcaaccacct gatggggaat agcgtcaccg
     2941 tcaccggact gctcggcgga aacgacatcg cagaggccgt ccgggggcat gtccgcggga
     3001 cgctgtacat cccctccgtc gccttacgag acgcggggga cctgttcctg gacgggcttt
     3061 ctccggagga ggtctcgcgg cggaccgggg cgccggtcgt cctcttcgag ccgacgccca
     3121 ggggattcca cgacgccgtc tacccacgga accggccgga atatcattga catttccttc
     3181 cgcagccggt aaattcagtg atcctgctcg gagggatggg cgattagctc agatggttag
     3241 agtacctgct tgacatgcag ggggtcactg gttcaagtcc agtatcgccc accatttctc
     3301 tccctcgaac ggcgcgcatc acggacggca cgtcaccgtg tcgccagtgc gcggggatat
     3361 tttttttttg ccggaaatcc gcaaggagtc tgcgcaggga tgacgctcct cgagctggcc
     3421 aggaaagaag ggaaggcgaa ggtcgccatc gccgccaggg tggatggcgt tgtgatggat
     3481 ctcgcgcgtc cgctgtccga cggagcgaag gtcgaatggg tccttccggc cgacccggac
     3541 ggggtggaga tcctccggca cagcacggcc cacgtcatgg ccgccgccgt caaggaactc
     3601 ttcccgagag cgctgatcac gatcggcccg gccatcgaaa acgggttcta ctacgacttc
     3661 gacgtcgaga cgccgttcac cccggaggac ctcgtccgga tcgaggatcg gatgcgcgag
     3721 atcgtcaagg ccgatcatcc gttcgttcgt gaagaggcga cgaaggagca ggcgcgcgtc
     3781 ctgttccccg gagaaccgta caaggaggag ctgctcgccg ccatccccga cgagaccgtt
     3841 tccctctacc gtatgggaaa cttcctcgac ctgtgccggg ggccgcacgt gccggggacg
     3901 ggccgcgttg gcgcgttcca tctgatgaat accgccgggg cctactggcg cggagattcg
     3961 aagaacaaga tgctcacgcg aatctacggg atcgccttcg cgtcgaaaaa ggaactcgac
     4021 gagcacctgc ggatcctcga ggaaatcaaa aagcgcgacc atcggaagat cgggcgggag
     4081 ctcgacctgt tcagcgtgac ggacgacatc gggccggggc tcatcctttg gcacccgaag
     4141 ggatcggtcg tccgcagggt catggaagat ttctggcggg atgagcacac gaaggcgggg
     4201 tacgacctgg tcttctcccc gcacatcgcg cgtctcgacc tttggcggat cagcgggcac
     4261 acggatttct accggcaggc gatgttctcc ccgatcgaca tcgaggggca ggaataccag
     4321 ttgaagccg
//
LOCUS       DASVJL010000106         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1142160, whole genome shotgun sequence.
ACCESSION   DASVJL010000106 DASVJL010000000
VERSION     DASVJL010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142160"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VF357_01990"
     CDS             complement(<1..702)
                     /locus_tag="VF357_01990"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522944.1"
                     /translation="MKVAVPREIREGERRVALVPESCRKLVKAGIEVTVESGAGSAAF
                     FPDDAFREAGASVGENAASVLGRADFILKVQAPAPYPSPGAHEVDAMREGAILLCTLM
                     PSRHPDAVEKLAARRITSFATDRIPRITRAQSMDTLSSMANISGYKAVLVAANALPRY
                     FPMLTTAAGTVLPAKVFVIGAGVAGLSAIATARRLGASVEATDTRPVAKEQVESLGAR
                     FAGVDTSEIAQDASGY"
     gene            981..>1620
                     /locus_tag="VF357_01995"
     CDS             981..>1620
                     /locus_tag="VF357_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX7522945.1"
                     /translation="MKGKAWRVAVPVLIAGLLAILPVPDGLTPNAWYYFAIFAAVVAG
                     LILEPIPAAAIGVIGVTLAASLLLVAPPPSKPAKPAADKPAAVVTDNAPKAATAVKAA
                     VTPKDNAGQAPSSAVPAGKPAGPPAAAAPSKGLTPADAIKWALSGFSDSTVWLIFVAY
                     MFAMGYEKVGLGRRLGLGLVEKLGKRTLGLGYAIALFDLVLAPFIPSNTARSG"
BASE COUNT          258 a          550 c          537 g          275 t
ORIGIN      
        1 gtatcccgag gcgtcctggg cgatctccga ggtgtcgacc cccgcgaacc tggcgcccag
       61 gctctccacc tgctccttcg ccacggggcg cgtgtcggtg gcctccacgc tggccccgag
      121 gcgccgcgcg gtggcgatcg ccgagagccc cgccactccc gcgccgatca cgaagacctt
      181 cgcgggaagc acggttccgg cggcggtcgt gagcatgggg aagtacctgg ggagcgcgtt
      241 ggcggcgacc aggacggcct tgtacccgga gatgttcgcc atggaggaga gggtgtccat
      301 cgattgcgcc cgggtgatgc gggggatccg gtccgtggcg aaggaggtga tccggcgggc
      361 cgccagtttc tccaccgcgt cggggtggcg cgagggcatc aaggtgcaaa ggagaatcgc
      421 cccctcgcgc atcgcgtcga cctcgtgcgc gccgggggac gggtaggggg cgggcgcctg
      481 cactttcagg atgaagtccg ccctgcccag caccgatgcg gcgttttccc cgacggaggc
      541 gcccgcctct cggaaggcgt cgtcggggaa aaacgccgcc gaccccgcgc cgctctccac
      601 cgtcacctcg atccccgcct tcacgagttt ccgacacgac tcggggacga gggcgacccg
      661 ccgttccccc tcacggatct cccttggaac cgcgaccttc atggcacccc ccgcgacgcc
      721 gtcggctgaa ggacagtgaa ccgtagatga atatattagc ggagaagcgc tccgcagcgt
      781 attccccccg cggaaatttt acgatggacg tgtcggccgg gacccaaacg gttctttggg
      841 ggacgaaccc tttcctgtat actgtataca caataaaagg tgcccattcg ccttttctgc
      901 accgatggca aggatccttc gcatcgttcc cgcgtgagat cgggggcgga ggccccatca
      961 atcgttggaa gggaggcaga atgaaaggga aagcctggcg agtcgccgtt cccgtcctga
     1021 tcgccggttt gttggcgatt cttccggttc ctgatgggct cacaccgaac gcctggtact
     1081 atttcgcgat cttcgccgcc gtcgtcgccg ggctgatcct cgagccgatc cccgccgcgg
     1141 ccataggggt catcggcgtc actctggccg catcgctcct gctggtcgct ccgcctcctt
     1201 cgaagcccgc gaaacccgcg gcggacaaac cggcggccgt cgttacggac aacgccccga
     1261 aggcggcaac cgccgtaaaa gcggccgtga cgcccaagga taacgccggg caggcaccgt
     1321 cctccgccgt cccggcaggg aagcccgccg gccccccggc ggcggccgcc ccgtcgaaag
     1381 gcctcacccc ggcggatgcg atcaagtggg cgctgagcgg tttctcggac agtacggtat
     1441 ggctcatctt cgtcgcctac atgttcgcga tggggtatga gaaggtcggg ctcggacggc
     1501 gcctcgggct cggcttggtg gaaaagctcg gcaagagaac cttggggctc gggtacgcga
     1561 tcgcgctgtt cgatctcgtg ctggccccct ttatcccttc caacacggcg cgtagcggcg
//
LOCUS       DASVJL010000107         8181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_485407, whole genome shotgun sequence.
ACCESSION   DASVJL010000107 DASVJL010000000
VERSION     DASVJL010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485407"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..885)
                     /locus_tag="VF357_02000"
     CDS             complement(97..885)
                     /locus_tag="VF357_02000"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX7522946.1"
                     /translation="MPLSGKRILVTRSVEQAGELAALVRRAGGIPVLFPTIRLTHPED
                     CGPLDREIGRLSSFDWILFASANAARFFCERASRLGVGSWPGTLRVASVGPGTTKELA
                     ARSVPVHRTAGKHTAEGLIEALLPAGIRGKRFLIPRAEEGREILPDAIEREGGAVVCV
                     VAYRNGLAEKDEAVAGEIVSRPPDVCTFASPSAFRNLFLLLGDGTARSVLSRTRIAVI
                     GEVTARAVERRDFRVDIVPETYTLKGMVDAVQAFFAAHRSADPG"
     gene            complement(890..1828)
                     /gene="hemC"
                     /locus_tag="VF357_02005"
     CDS             complement(890..1828)
                     /gene="hemC"
                     /locus_tag="VF357_02005"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX7522947.1"
                     /translation="MAASDIIRLGSRGSTLALWQAEHVRAEVERHTGRKVEITRIKTT
                     GDMILDVPLSKVGGKGLFVKEIEEALLSNRIDLAVHSMKDVPTDLPDGLEISCITRRE
                     DPRDAFLSVKHARFEDLPVGAHVGTSSLRRQTQLLGLRPDLSIDQLRGNLDSRIRKME
                     EGKYDAILLAAAGLRRLGWDIKIRQYIPADVSIPAIGQGALGIEIRSDDGRTREAVAF
                     LDDRETSLAVRAERGFLKRLEGGCQVPIAAHGTVNGDTVSLSGLIGKPDGSRILRGIR
                     SGSVSDPEAVGVALAGELLSRGGREILDEVYRQAGS"
     gene            complement(1815..3152)
                     /gene="hemA"
                     /locus_tag="VF357_02010"
     CDS             complement(1815..3152)
                     /gene="hemA"
                     /locus_tag="VF357_02010"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF000744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX7522948.1"
                     /translation="MSEIIIVGLNHRTAPVEVRERLAFPADTVGHALRGLRERDGISE
                     GVILSTCNRVEVCVLSETGYKGVEHVKDFLAGFHGVPIGELSDHLYHHLGEEAVRHLF
                     RVSSSLDSMVLGEPQILGQVKDAYGYACEFKTIGPVLDKFFTKAFSVAKRVRTETRVA
                     NSAVSVSYAAVELAKKILGDLQDKVVMLIGAGEMCELAARHLLSAGAKGILVTNRTFE
                     RAVHLAEEFDGTAVRFEELATHLKRADIILSSTGAPHFILQREDVESVIRIRRNRPMF
                     FIDMAVPRDIDPDANEIDNVYVYDIDDLNNVIETNLEERQREAALAEEIVVAEVAGFR
                     RWLDSQQVTPTIVSLRRKFDEVRQAEVAKALSALGTADPKTRKVVESLASSILNKVLH
                     SPIASLKRETDGRSPMEMVTAVREIFDLPEEEAEEAPEAAERAPGRGGNVGSQ"
     gene            complement(3160..3987)
                     /gene="ccsB"
                     /locus_tag="VF357_02015"
     CDS             complement(3160..3987)
                     /gene="ccsB"
                     /locus_tag="VF357_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEX7522949.1"
                     /translation="MHTALLKVTALFYFVGALAYLHFIFTLSERSAKLGRMLLLIGAI
                     LHGAGFVARYFVAGYTPITSLFESLSFSAFAIVCVFLAFELRYHLRVLGAFVAPLAFA
                     FSVSAAFLPGEVRALAPALNSYWLPVHVILLFFGNAVFAVAFGAAIMYLLMERELKHK
                     KLGAIFKRLPSLDVLDDINYRCLTIGFPLLTLGIITGSIWAEYAWGSYWSWDPKEVWS
                     LITWLLYAALLHGRMTVGWRGRKAAILAIVGFCAVLFTFLGVNLLLPGLHTYTNLSG"
     gene            complement(4106..6007)
                     /locus_tag="VF357_02020"
     CDS             complement(4106..6007)
                     /locus_tag="VF357_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEX7522950.1"
                     /translation="MHALSLFLLVLLSTWAWVPSSQATDNAYLAELKGRARSLALHED
                     RYWRILLHVRPGIAGTRSLVDDPRFFLSPSGKTDPAAELSATLDGLFDNTAAPPYFCR
                     FPARYAWLALSLEIDPTRIPRPPPCPEVDNILRTIDGQSAAMVFASGYVNSPASMFGH
                     TFLRIDSRLKNPLLSFAVNYAARTDPSDGGIAFTAKGMFGGYQGFYSVMPYYDKVREY
                     SNIDQRDLWEYSLNLTPDQVRRMLLHLVEMKDIATDYFFFDENCSYELLFLLDAAHPE
                     ARLTETMDRYWVIPMDTVRAVIEAGLVDNVVWRPSRALQIRHARSLLPEREAGLAVEI
                     VDGAAPAGSVLSEPVPDATKAQTLALAALLAQYRIAKKGMEHKVFQERFHAILSARTR
                     LGTVEEVAKPLPVPDPPEDGHRSARARIGGGWRGSEGFLEAGIRPAYHDYTDPPEGYT
                     QGSLIEFLSGAVRWYPETDRIRLSQLDLLRIESLDPFDPIFRKKSWKVRMGFETRDFT
                     EERDALVSFAGCGIGGAIRLREGAVASLLGEVEGDFSRRYHSAYRIGGGATVGITAEP
                     VRGWRLRVEGRGIWGALGETREGPDLSASLRQGFRIGRDLSLTADVSRSVTRRVQRTE
                     GSLSLNRYF"
     gene            complement(6056..6532)
                     /locus_tag="VF357_02025"
     CDS             complement(6056..6532)
                     /locus_tag="VF357_02025"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HEX7522951.1"
                     /translation="MKRLLLVAALVLVLAVPAIAAPPSQMNYGCGLGATVFKDGQAND
                     SLLLQLVATFLNGLCGNGTFGITSGTSDCAPPAKTANIDRLNEFAYRNLDDLARDIAA
                     GKGETVATVADLLSVPVESRPTFYRNLQARFTEIFPSPAVETAHVVEAITTIASQG"
     gene            complement(6613..7491)
                     /locus_tag="VF357_02030"
     CDS             complement(6613..7491)
                     /locus_tag="VF357_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7522952.1"
                     /translation="MTDRLRVLPFLAILALALSAWGCRSPFFYPTRDHVPVPGLENTA
                     RQDVRFASEDGVPLHGWLLSPRDRGSRGTILFLHGNAENISTHVRSVLWLVREGYTVF
                     AFDYRGYGWSGGEAPDIPGVHRDARAALSKILTLPGISPDRLVVFGQSLGAAVAVHTV
                     ATASSARRPRALILDSPFAGYRRIVRDKLSSSIITWPLAWPASWFFDDEYSPERWIGK
                     IAPVPVIVIHGTADRVVPYAHGKLLYDLASDPKGIWTVEGGGHATALRNPEVRGQFLA
                     FLASVLPPEGEGCSSR"
     gene            complement(7488..>8181)
                     /locus_tag="VF357_02035"
     CDS             complement(7488..>8181)
                     /locus_tag="VF357_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522953.1"
                     /translation="MADEEGIPVHRKNSMLVARFYVCLAANRYIDDTQCGYRLYPLSV
                     VESIALHKERYVMETELLLKVGDSGIPIRSLPIPAVYLPDQKTYFRSVPDVAAISVYV
                     ISYIMVKWALELWRPGEVNTYKGPGTGRDRFFLSPAVDRAFESLMVLTCMPLSAMYGI
                     LYFLTRSLFGIRVFEGLKGCGVPVGTVFLSTMLLPLLLVVSIVDLVGNRVGLRPGLTS
                     RFVTKWYPNLWK"
BASE COUNT         1460 a         2704 c         2722 g         1295 t
ORIGIN      
        1 cggaccatcc ggcgcagcgt ctcgttccgc cgcaggcggc ggggccggta ttcggggtat
       61 tgcatcggat ccctcctggt gcggctcggc cgcccgttac ccggggtcgg ccgatcggtg
      121 ggcggcgaag aacgcctgga cggcgtccac catccctttc agcgtatacg tttcgggtac
      181 gatgtccacc cggaaatccc tgcgttccac ggcccgcgcg gtcacctcgc cgatcaccgc
      241 gatgcgcgtc cgggagagca cgctcctcgc cgtcccgtcc ccgaggagaa ggaagaggtt
      301 ccggaacgcc gagggcgacg cgaaggtgca gacgtcggga gggcgggata cgatctcccc
      361 ggccaccgcc tcgtccttct ccgcgagacc gttccggtag gcgacgacgc acaccaccgc
      421 tccgccttcc cgctcgatcg cgtcgggaag gatctccctc ccctcctcgg cgcgcgggat
      481 gaggaaacgc tttccccgga tccccgcggg aaggagcgcc tcgatgagcc cttcggcggt
      541 gtgctttccc gccgtccggt gcaccgggac ggacctcgcg gcgagctcct tcgtcgtccc
      601 cggccccacg ctggccacgc gaagggtccc cggccaggat ccgaccccga gccgggacgc
      661 gcgctcgcag aagaaccggg ccgcgttggc gctcgcgaag aggatccagt cgaaggagga
      721 gaggcggccg atctcccggt cgagagggcc gcaatcctcg ggatgcgtca gccggatcgt
      781 ggggaagagg accggaatgc cgcccgcgcg ccggaccagg gcggccagtt ctccggcctg
      841 ctccacgctg cgtgtgacga ggatccgttt ccccgagagc ggcatcgctt cacgaaccgg
      901 cctgtcggta cacctcgtcg aggatctccc tgccgccccg cgacaggagc tccccggcga
      961 gcgcgacccc gaccgcctcc gggtcggaga cggaaccgct ccggattccg cgcaggatcc
     1021 gcgagccgtc gggcttcccg atcagccccg agagggagac cgtgtccccg ttcaccgttc
     1081 cgtgcgccgc gatggggacc tggcacccgc cttccagccg cttcaggaag ccgcgctccg
     1141 cccggaccgc cagggacgtc tcccggtcgt ccaggaacgc caccgcctcg cgggttcttc
     1201 cgtcgtcgct ccggatctcg atcccgagcg ccccctgtcc gatcgccggg atggaaacgt
     1261 cggcggggat atattgacgg atcttgatgt cccacccgag ccggcgaagt cccgcggcgg
     1321 cgaggaggat cgcgtcatat ttcccctcct ccatcttccg gatgcgcgag tcgaggttcc
     1381 cccgcaattg atcgatcgaa aggtcggggc gcaggcccag cagctgggtc tggcggcgca
     1441 gggagctcgt ccccacatgg gcgccgaccg gaaggtcctc gaaccgcgcg tgcttcacgg
     1501 acaggaaggc gtcgcgcggg tcctcccgtc gcgtgatgca cgagatctcg agcccgtcgg
     1561 ggagatcggt ggggacgtcc ttcatcgagt ggacggcgag gtcgatccgg ttcgacagca
     1621 gcgcctcctc gatctccttg acgaagagcc ccttcccgcc caccttcgac aatgggacgt
     1681 cgaggatcat gtcgccggtc gtcttgattc tggtgatctc cactttccga ccggtgtgcc
     1741 gctctacctc cgcccggacg tgctccgctt gccagagggc gagggtgctc ccccgactgc
     1801 cgagccggat gatgtcactg gctgccaacg tttcctcctc gtccgggggc gcgctccgcc
     1861 gcttccggcg cctcttccgc ttcctcttcc gggaggtcga agatctcccg caccgccgtc
     1921 accatctcca tcgggctgcg gccgtcggtc tcccgcttca gcgacgcgat cggtgagtgc
     1981 agcaccttgt tcaagatcga ggaggcgagc gactcgacca ccttccgcgt cttcgggtcg
     2041 gccgtcccca gcgcggagag cgccttggcg acctccgcct gccgcacctc gtcgaactta
     2101 cgccgaaggg agacgatcgt cggggtgacc tgctgcgaat cgagccagcg gcggaagccc
     2161 gccacctcgg cgacgacgat ctcctcggcc aacgcagcct cgcgctgccg ctcctcgagg
     2221 ttcgtctcga tcacgttgtt caggtcgtcg atgtcgtaga cgtagacgtt gtcgatctcg
     2281 ttggcgtccg ggtcgatgtc gcgcggaacc gccatgtcga tgaagaacat cggccggttc
     2341 ctgcggatcc ggatgaccga ctccacgtct tcccgctgaa ggatgaagtg tggggcgccg
     2401 gtcgaggaga ggatgatgtc ggcgcgcttc aggtgggtgg ccagctcttc gaagcgcacg
     2461 gccgtgccat cgaactcctc ggcgaggtga acggcgcgct cgaaggtccg gttcgtgacg
     2521 aggatcccct tggcgccggc ggagagcagg tgccgcgccg ccagctcgca catctccccc
     2581 gccccgatca gcatcaccac cttgtcctgg aggtcgccca ggatcttctt cgcaagttcg
     2641 acggcggcgt acgagaccga gacggcgctg ttcgcgaccc gcgtttccgt gcggacccgt
     2701 tttgcgaccg agaacgcctt cgtgaagaac ttgtcgagga ccggcccgat cgtcttgaac
     2761 tcgcaggcgt acccgtaggc gtccttcacc tgcccgagga tctgcggctc cccgagcacc
     2821 atcgaatcga ggctgctgga gacgcggaac aggtgccgca ccgcctcttc cccgaggtgg
     2881 tggtagaggt ggtccgacag ctcgccgatc gggacgccgt ggaacccggc gaggaaatcc
     2941 tttacgtgct ccacgccctt gtatcccgtt tccgaaagga cgcagacctc gacgcgattg
     3001 caggtggaga ggatgacccc ctcggagatg ccgtcgcgct cccgcagccc ccggagggcg
     3061 tgccccaccg tgtccgccgg gaacgccagg cgctcccgga cctccaccgg cgcggtgcgg
     3121 tggttcaagc cgacgatgat gatctcgctc atgcgccgat cagcccgaaa ggttcgtgta
     3181 ggtgtgcaga cccggcagga gaaggttcac gccgaggaag gtgaacagga ccgcgcagaa
     3241 cccgacgatg gcgaggatcg ccgccttccg cccgcgccag ccgacggtca tccgaccgtg
     3301 gagcagcgcg gcgtagagca gccacgtgat gagggaccag acctctttcg ggtcccagct
     3361 ccagtaggag ccccaggcgt actccgccca gatcgatccc gtgatgatcc ccagggtgag
     3421 cagcgggaaa ccgatcgtca ggcaccggta gttgatgtcg tcgagcacgt cgagcgacgg
     3481 cagccgcttg aagatcgcgc ccagcttctt gtgcttcagc tccctctcca tcagcaggta
     3541 catgatcgcc gcgccgaacg ccacggcgaa gacggcgttg ccgaagaaaa ggaggatcac
     3601 gtggacgggg agccagtacg agttgagcgc gggggccagc gcccggacct cgccgggcag
     3661 gaacgccgcg gagacgctga acgcgaaggc cagcggcgcc acgaacgcgc cgagcacccg
     3721 gaggtggtac ctgagttcga aggcgagaaa gacgcacacg atcgcgaagg cggagaagga
     3781 gagcgactcg aagaggcttg tgatcggggt gtaccctgcg acgaaatagc gcgcgacgaa
     3841 ccccgccccg tggaggatgg cgccgatcag cagcagcatc cgcccgagct tcgcacttcg
     3901 ttcgctcagc gtgaagatga agtggaggta cgccagcgcc ccgacgaagt agaagagggc
     3961 cgtgaccttg agcagggccg tgtgcatcag gacatattaa accacccgtg gcgcccggaa
     4021 caagcacgga atccggaggc gcccggattc catgaggaat cgccggaccg agacccgccg
     4081 gaagagatgg agacgatcgg gcttgtcaaa agtaccggtt gagggaaaga gacccctcgg
     4141 ttcgctgaac gcggcgtgtc accgaccggg acacgtccgc ggtcaacgac aggtccctgc
     4201 cgatacggaa accttgccgg agggaggccg acaggtcggg cccctcccgg gtctctccca
     4261 acgctcccca gatcccccgt ccctccaccc gcaaccgcca cccccggacc ggctccgccg
     4321 tgatcccgac ggttgcgcct cccccgatcc ggtaggcgct gtggtagcga cgcgagaaat
     4381 ccccttccac ctcccccaga agggaggcga ccgccccctc ccgaagccgt atcgcccctc
     4441 cgatcccgca cccggcgaac gaaacgaggg cgtcccgctc ctcggtgaaa tcccgtgttt
     4501 cgaagcccat gcgaaccttc cacgatttct tccggaagat cgggtcgaac gggtcgaggg
     4561 actcgatccg gaggaggtcg agctgcgaca accggattcg gtcggtctcc gggtaccacc
     4621 ggaccgctcc cgacaggaat tcgatcaatg acccctgggt gtacccttcc gggggatcgg
     4681 tgtagtcgtg gtaggccggg cggattcctg cctccaggaa tccttcgctc ccccgccacc
     4741 cgccgccgat gcgggcccgg gcgcttcgat gcccgtcttc cgggggatcg ggaaccggga
     4801 gaggcttcgc tacctcctcc acggtgccga gccgcgtccg ggcggacagg atcgcgtgga
     4861 accgctcctg gaagaccttg tgctccatcc ccttcttcgc gatccggtac tgcgccagca
     4921 gcgcggcgag ggcgagcgtc tgcgctttcg tagcgtcggg aaccggctcg gagaggaccg
     4981 atccggcggg ggccgccccg tcgacgatct ccacggcaag ccccgcttcg cgttccggta
     5041 gcagggagcg ggcgtggcga atctggagcg ccctggaggg acgccacacg acattgtcga
     5101 caagccccgc ttcgatgacc gcgcggaccg tgtccatcgg gatgacccag taccggtcca
     5161 tcgtttcggt gagccgggcc tccggatgcg ctgcgtcgag gaggaagagc agttcgtacg
     5221 agcagttctc gtcgaagaag aagtaatccg tcgcgatatc cttcatctcg acgaggtgga
     5281 ggagcatgcg gcgcacctgg tcgggggtca ggttcaggga gtactcccag aggtcgcgct
     5341 ggtcgatgtt ggagtactcc cgcaccttgt cgtaataggg catgacggaa tagaaccctt
     5401 ggtatccccc gaacatcccc ttggccgtga aggcgatccc tccgtccgac gggtccgtac
     5461 gggccgcgta gttgaccgcg aaggaaagga gagggttctt cagcctcgaa tcgatccgga
     5521 ggaacgtgtg gccgaacatc gaggcgggag agttcacgta gcccgacgcg aagaccatcg
     5581 cggcggattg gccgtcgatg gtccgcagga tgttgtccac ctcggggcac ggcggcggcc
     5641 gcgggatccg ggtcgggtcg atctcgagcg acagggcgag ccaggcgtac cgggccggga
     5701 agcggcagaa gtaggggggg gcggcggtgt tgtcgaacag cccgtcgagc gtggcggaca
     5761 gttccgccgc cggatcggtc ttccccgacg gggagaggaa gaagcgggga tcgtcgacga
     5821 ggcttcgcgt acccgcgatt cccggccgga cgtgcagaag aatccgccag tagcggtcct
     5881 cgtggagagc gagggatctc gcccgtccct tcagttcggc gagatatgcg ttgtcggtgg
     5941 cctgcgagga tggaacccaa gcccacgttg agagaaggac gagaaggaaa agagaaaggg
     6001 catgcacccg gtggatgcat gccctcgtcg tggtatcgct accgtccaga agcgatcaac
     6061 cctgggacgc gatcgtcgtg atcgcttcga ccacgtgggc ggtctcgacc gccggggacg
     6121 ggaagatctc cgtgaaccgg gcctggaggt tccggtagaa ggtcggccgg gactcgaccg
     6181 ggacgctcag gaggtcggcg accgtggcca ccgtctctcc cttccccgcc gcgatgtcgc
     6241 gggcgaggtc gtcgaggttc cggtaggcga actcgttcaa ccggtcgatg ttggcggtct
     6301 tcgccggagg agcgcaatcc gaggttcccg aggtgatgcc gaacgtcccg ttcccgcaga
     6361 gaccgttcag gaaggtggcg accaactgga gcagcaggct gtcgttggcc tgcccgtcct
     6421 tgaacacggt ggccccgaga ccgcacccgt agttcatctg gctcggcggg gccgcaattg
     6481 caggaacggc gaggacgagc accaacgcgg cgacgagaag aagtctcttc atatccgacc
     6541 ctcctgatgg aatgattact tgcagaacac actaccccgc gggtatttca ctgtcaacca
     6601 cgaacgccct tttcaccggg atgagcagcc ttcgccctcc ggcgggagga ccgaggcgag
     6661 aaacgcgagg aactggcccc gcacctcggg attcctcagg gccgtcgcgt gcccccctcc
     6721 ttccaccgtc cagataccct tcgggtccga cgccagatcg tagagcaact tcccgtgggc
     6781 gtacggcaca acccggtccg cggtcccgtg gatcacgatg acgggaaccg gggcgatctt
     6841 cccgatccac cgttcggggc tgtattcgtc atcgaagaac cagctggcag gccatgccaa
     6901 cggccacgtg atgatcgagg aggaaagttt gtcccggacg atccggcggt agccggcgaa
     6961 ggggctgtcg aggatcaggg cgcgcggacg ccttgccgaa gaggcggttg cgacggtgtg
     7021 gacggcgacg gccgcaccga gggattggcc gaacacgacg agccgatccg gcgagatccc
     7081 tggaagggtg agaattttcg acagcgccgc gcgggcgtcc cggtggacac ccgggatgtc
     7141 cggcgcctcc ccacccgacc agccgtaccc tcgataatcg aaggcgaaga ccgtgtaccc
     7201 ttcccggacg agccaaagga ccgaccggac gtgggtgctg atgttttccg cgttgccgtg
     7261 gaggaagaga atcgtcccgc gggaaccccg gtcgcgcggg gacaggagcc agccgtggag
     7321 cgggacgccg tcctccgacg cgaaccggac atcctgtcgc gcggtgttct ccagtccggg
     7381 aaccggaacg tgatcccgcg tcgggtagaa aaagggggag cggcaccccc acgccgaaag
     7441 ggcgagcgcg aggatcgcaa ggaacgggag aacgcggagg cgatcggtca tttccagaga
     7501 ttcgggtacc atttcgtcac gaaccgggag gtaagtcccg ggcgcagccc gacacggttc
     7561 ccgacgaggt cgacgatgga aacgaccagc aacagcggaa gcagcatcgt cgagagaaac
     7621 accgttccga cagggactcc gcatcccttc aacccctcga acacccggat accgaacagg
     7681 gatcgcgtga ggaaatacag gatgccgtac atggcgctca acggcatgca ggtcagcacc
     7741 atcaggctct cgaacgccct gtccacggcg ggggaaagga aaaaccggtc ccgcccggtg
     7801 ccgggcccct tgtaggtgtt gacctccccc gggcgccaca gctcgagcgc ccacttgacc
     7861 atgatatagg agattacgta gacggaaatg gccgccacat ccggtacgga acggaagtac
     7921 gtcttctgat ccggaagata gacggcaggg atcggcaacg agcggatcgg aattcccgag
     7981 tccccgactt tcaggagaag ttcggtttcc atcacatagc gttccttgtg aagcgcgatg
     8041 gactccacca cggacagcgg gtaaagacga tacccgcatt gggtatcgtc gatgtagcgg
     8101 ttggccgcga ggcagacgta gaatctggcg accagcatcg aatttttccg gtgaacgggg
     8161 atcccttctt catcggccat c
//
LOCUS       DASVJL010000108         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_110710, whole genome shotgun sequence.
ACCESSION   DASVJL010000108 DASVJL010000000
VERSION     DASVJL010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110710"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VF357_02040"
     CDS             complement(<1..388)
                     /locus_tag="VF357_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7522954.1"
                     /translation="MHGKRTHILSYALTLLLSLALASAGVAADKEIRIGAAYPLSGNI
                     AATGLDCLHGAELAVDIINGKYPDLNLPLAKSEGLPNLGGAKLKLIVADTKGEPRNGQ
                     AEAERLVTQEKVVAMIGAYQSSVTKTA"
     gene            complement(458..1192)
                     /locus_tag="VF357_02045"
     CDS             complement(458..1192)
                     /locus_tag="VF357_02045"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX7522955.1"
                     /translation="MAKKVGDNLVRTILDRIFQGKLTDGERLPSIEKMTREYGMSAVS
                     VREAIHKLSMMGLVHIRQGGGTFLDRNIPSIIDILDARKYVEVAAGYLAARNATAEEQ
                     ARLGRIVAAMEEDFRNRDYVAYSKKDLEFHLAIARMSRNVLLSAFLENIQDLLYYLQE
                     RTHMLRGTIEKANRFHPQIAKAIRKGDGKKAQELIAAHIEAVKRAWEAYGRTERSEAI
                     GTPLPRRTGAAAEAKSRLGTVRKKAG"
     gene            complement(1267..1968)
                     /locus_tag="VF357_02050"
     CDS             complement(1267..1968)
                     /locus_tag="VF357_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7522956.1"
                     /translation="MIGGLDFSVVWDNLSYFFIGRYPKGQLGGVGLTLYLAVVSLALS
                     FLGGLVLGLLSVSRNRLLRLGSMAVIQTIRGMPLLMVIFWMFFLLPALLGGGMSAAWT
                     IIVALTLFTSAYMSEIVRAGIVGIPKGQMEAAISTGLSHGQAMIHIILPQALRNMIPS
                     FVNQFVSMIKDTSLAFIVGVTELTQIATQINNRTMLYPTEIFLFIAVIYFIMCFAFTE
                     LSRWLERRLAYRKSI"
BASE COUNT          385 a          674 c          659 g          413 t
ORIGIN      
        1 tcgccgtctt ggtgacggag ctctggtacg ccccgatcat cgccacgacc ttttcctggg
       61 tgaccagccg ctccgcctcg gcctgcccgt tccggggttc ccccttcgtg tcggcgacga
      121 tcagtttcag cttggcgccc ccgaggttgg gaagtccttc cgatttcgcg aggggcaggt
      181 tgaggtcggg gtatttcccg ttgatgatgt ccacggcgag ctccgccccg tgcaggcagt
      241 cgaggcccgt ggcggcgatg ttccccgaca gggggtaggc ggcgccgatc ctgatctcct
      301 tgtccgcggc gactcccgcg gatgccagcg ccagcgacaa cagcagagtc agagcgtacg
      361 acagaatgtg ggttcgtttc ccgtgcatcg tgccctcctt tgtggtgtac tcccgttgat
      421 gttgcacagc cttcccgttc catccctgcg ggatccgcta ccctgccttc ttccgaactg
      481 tcccgagccg cgacttcgcc tcagccgccg cgccggtccg ccgcggtaac ggcgtgccga
      541 tggcctcgct tctctcggtc cggccgtacg cctcccatgc gcgtttgacc gcctcgatat
      601 gcgccgcgat gagctcctgt gctttttttc cgtccccctt ccggatcgct tttgcgatct
      661 gagggtggaa acgattcgcc ttctcgatcg tcccccgcag catgtgggtc cgctcctgca
      721 ggtagtacag gagatcctgg atattctcga ggaaagcgga tagcaggacg ttcctcgaca
      781 tccgcgcgat ggcaaggtgg aattcgaggt ctttcttcga ataggccacg tagtcgcggt
      841 tccggaaatc ctcctccatt gccgccacga tccggccaag ccgggcttgc tcctcggccg
      901 tcgcgtttcg ggcggcgaga taaccggcgg ccacctcaac gtactttcgg gcgtcgagga
      961 tatcgatgat ggagggaatg ttccggtcga ggaaggtacc gccgccctgg cggatgtgga
     1021 cgagccccat catggagagc ttatgaatcg cctcccggac ggagacggcg ctcatcccgt
     1081 actcccgggt cattttctcg atggacggga ggcgctcccc gtcggtgagc ttcccctgga
     1141 agatccggtc caggatggtg cggacgaggt tgtcgccgac cttcttcgcc accggccccc
     1201 cgggagtcat aggatgatcc catcttccga ttcacttcca tggatgtcaa ggggaaacgc
     1261 gaggggtcag atcgacttgc gataggccaa tcgccgctcc agccatcggg acagctccgt
     1321 gaaggcgaaa cacatgatga agtagatgac cgcgatgaac agaaagatct ccgtgggata
     1381 gagcatcgtc cggttgttga tctgggtggc gatctgggtg agctccgtca cccccacgat
     1441 gaacgccagc gaggtgtcct tgatcatcga cacgaactgg ttgacgaagg aagggatcat
     1501 gttccgcagc gcctgtggga ggatgatgtg gatcatcgcc tgcccgtgag acagcccggt
     1561 cgagatcgcc gcctccatct gccccttcgg gatcccaacg attcccgcgc gcacgatctc
     1621 ggacatgtag gcggaggtga acagggtaag cgcgacgatg atcgtccacg ccgcggacat
     1681 cccgcccccc aacagcgccg gcagaaggaa gaacatccag aagatgacca tcagcagcgg
     1741 catcccgcgg atcgtctgga tgaccgccat ggatcccaac cggagcagcc ggttccggga
     1801 gacgctcagc aggcccagta ccagcccgcc gaggaaagag agcgcgaggg ataccacggc
     1861 gaggtaaagg gtgagcccca caccgccgag ctggcccttc gggtaccggc cgatgaagaa
     1921 gtaggacaga ttgtcccaga cgacggaaaa gtcgagcccg ccgatcacgt cattgcccct
     1981 tgaggggctg gaggaccttc ttgtcgtaaa gggtcatcag cccggtgatg gtgaaggaaa
     2041 gtgcaaggta taccagggtc gccgccgaaa acgcctcgaa ccccttgaag gtgtagctct
     2101 ccacctgccg cgcctggtac atcagctccg c
//
LOCUS       DASVJL010000109         5575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1329094, whole genome shotgun sequence.
ACCESSION   DASVJL010000109 DASVJL010000000
VERSION     DASVJL010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329094"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VF357_02055"
     CDS             complement(<1..1031)
                     /locus_tag="VF357_02055"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7522957.1"
                     /translation="MSDRTPNRGSLLALAVLTAALLAVSVIACGKDPSSARAAGRDKA
                     QEPRLVRVVGAAVGRLPRTVTVTGTLAADEELVAGFKVAGRVSEITVDLGSPVRKGQV
                     LARLDPTDFRLRVEQAEAALRQVRAGLGLPRESSAESVDPEKTALVREARAVLEEARL
                     NRDRMVRLWEKDYIARSEVDASLSKLLVAESRYQAAMEEIRNRQELLAERSSGLDLAR
                     QQLADAVLSAPIDGAVRERRASVGEYLAAGAPVFGLVRVHPLRLRVAVPERDASSVRV
                     GQAVRVLLEGDPAVHDGRVARLSPSIQEQNRTLIVEAEVANRDGRLRPGSFARAEIVV
                     EADRNAVMVP"
     gene            1214..1576
                     /locus_tag="VF357_02060"
     CDS             1214..1576
                     /locus_tag="VF357_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7522958.1"
                     /translation="MSARESSLEVRSKIWLEMDGEPVFGQGREDLLRHIRRTGSINAA
                     AKKMGIPYRKAWTYIDAMERRMGFPLVSRKKGGAGGGESTLTPQAIILLEKFHSLKKN
                     FDKGVDRKFAELDFGGSR"
     gene            1581..2318
                     /locus_tag="VF357_02065"
     CDS             1581..2318
                     /locus_tag="VF357_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7522959.1"
                     /translation="MPAPQLILLAVCILAVAVLYSSVGHGGASGYLAVLALFSVAPVA
                     FKPTALILNILVSAVATVHFTRAGHFSWRLFWPFAAASIPFSFVGGYLTVPASVYNPL
                     LGIVLLASAVRLFSREDTEQAESGPPSTTVALGVGAALGFLSGLIGVGGGIFLSPLLL
                     LMNWGNAKEASAVAALFILINSISGLLGHVSSLQAIPSFVPVLAATALCGGITGSFFG
                     SRRLQGTSIVRILSVVLTIAGIKLLLV"
     gene            complement(2339..3136)
                     /gene="extS"
                     /locus_tag="VF357_02070"
     CDS             complement(2339..3136)
                     /gene="extS"
                     /locus_tag="VF357_02070"
                     /inference="COORDINATES: protein motif:HMM:NF040971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon c-type
                     cytochrome lipoprotein ExtS"
                     /protein_id="HEX7522960.1"
                     /translation="MDLRTSLLIIVLPIVLVVASPANGMPPACSSCHPVHFADRGTCI
                     GCHLGNDRTLRKDIAHLDLVPGRLLRYRFPGDPAVERGTQWIDRASCRRCHSIHGTGN
                     RLAGDLDRSAGRNPNKILASILQPVTFMPDFRFLEENAAEVVNALVAGARRDPDRKGE
                     VPSVVRFGDRDERSENVFVKRCGGCHRVLTRRLGGLGKGDAGPNLSGLLSRFYPRTSG
                     EGKAWTVERLRKWLDDPRRARAFARMPPVRMPRKEFTELEKTLRDDE"
     gene            complement(3112..3546)
                     /gene="extQ"
                     /locus_tag="VF357_02075"
     CDS             complement(3112..3546)
                     /gene="extQ"
                     /locus_tag="VF357_02075"
                     /inference="COORDINATES: protein motif:HMM:NF040970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome membrane protein ExtQ"
                     /protein_id="HEX7522961.1"
                     /translation="MKPPRQPDAGYVKSSPRFFRLIAGSGLLFTALVLAGAIFLPAPL
                     LGPADAGAPPNPAKSAWFLLWIQEVVSHGTSLVYPVLLLGLVFLFLPWLPLYGSVPAA
                     SWLPRTQRAVNLLALATFVAIVTLSVIAAVFRGPQWTFVRPF"
     gene            complement(3543..4163)
                     /locus_tag="VF357_02080"
     CDS             complement(3543..4163)
                     /locus_tag="VF357_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX7522962.1"
                     /translation="MSREFIRHLFPRVVLSRNLTIGYTLCLGGLAFTSFLLLGASGIL
                     LACYYQPSPERAYHSILFLESEVYGGLYLRSLHRLASHLFLGLIFLHTLRVLLTGAYQ
                     KPRDFNWLIGCGLLGLGVFAGYTGYLLPMDQLAFWATRTGMELLRALPFGGAIHDILV
                     PDGTGGGLSLLRFYVLHIAILPMGVLTLSYLHFYRVRKNKGVLPYL"
     gene            complement(4177..4569)
                     /locus_tag="VF357_02085"
     CDS             complement(4177..4569)
                     /locus_tag="VF357_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX7522963.1"
                     /translation="MADPRTSRRRFLGALIAFAGALAGLDRFLRPRPRRRKELLAVAL
                     ADLPPDGALVFREARVAVVRDGDGFHAISLVCTHLGCTVAVTPAGILCPCHGSRFDRR
                     GAVLEGPATRPLPLLPFDVRGERLIVTS"
     gene            complement(4524..>5575)
                     /gene="extO"
                     /locus_tag="VF357_02090"
     CDS             complement(4524..>5575)
                     /gene="extO"
                     /locus_tag="VF357_02090"
                     /inference="COORDINATES: protein motif:HMM:NF040968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome iron-sulfur cluster-binding subunit ExtO"
                     /protein_id="HEX7522964.1"
                     /translation="CHRGTAAIFGHVMGTRSREKDFVARSLSAVDKDFFAKNCGSCHI
                     SGCLDCHEGGGHRTARASPSRCLACHKGYYVGADYFGKAPREDSLRYQRGRVANGETF
                     LTMLPDVHAEAGLSCGACHTMRSLVEGKKAGKTCTDCHRWKGSSPEHRIAAHRDRLEC
                     YACHSAWAAQEYGTFFLRITESRSREDFWLKGNDTESEYLRSAYLRRQDAPPLGMNGR
                     GKISPIRPQFIAYYTDLRRDKAEGTENRLLAAEWKAFFPHTIRRGTVLCDGCHDAPRR
                     FLMEDPRDRIYRIREDGMTLESFWDRTGQRVVNGAFLPAESYRRLSARGPDFQRGYLE
                     RWRILVHHVDDSSAR"
BASE COUNT          942 a         1810 c         1884 g          939 t
ORIGIN      
        1 ggaaccatga cggcgttgcg gtcggcctcg accacgatct cggcgcgcgc gaacgagccg
       61 gggcgaagcc tcccgtcccg gttcgccact tcggcttcga cgatcagcgt gcggttctgc
      121 tcctggatgg acgggctcag gcgggccacg cggccgtcgt gcactgcggg gtctccctcc
      181 aacaggaccc ggaccgcctg cccgacccgc acggaggaag cgtcccgttc cggcaccgcc
      241 acccgaagcc ggagcgggtg gacccggacc aggccgaaca ccggagcgcc ggcggccagg
      301 tactcgccca ccgaagcgcg gcgttcccgc acggcgccgt cgatcggcgc gctcagcacc
      361 gcgtcggcca gctgctgtcg ggccaggtcg aggcccgaac tccgttcggc aagcaactcc
      421 tgacggttcc ggatctcttc catggcggcc tggtagcggc tctcggcgac cagcagcttg
      481 gagagggatg cgtcgacctc ggaccgggcg atgtaatctt tttcccagag ccgcaccatg
      541 cgatcccggt tgaggcgcgc ctcctcgagc accgcccggg cctcgcgcac cagcgcggtc
      601 ttctcgggat cgacgctttc ggccgagctt tccctgggga gccccagccc ggcgcgcacc
      661 tggcggaggg cggcttcggc ctgttccacg cgaagtcgga aatccgtcgg gtcgaggcgg
      721 gcgaggacct gccctttcct caccgggctg cccaggtcta ccgtgatttc gctgacgcgg
      781 ccggcgacct tgaagcccgc gacaagctct tcgtcggcgg cgagcgtccc ggtgacggtc
      841 accgtgcgag gaagccgccc caccgccgcg ccgacgacac gcaccaggcg cggctcctgg
      901 gccttgtcgc gccccgccgc gcgcgccgac gaaggatcct tcccgcaggc gatcacggaa
      961 acggcaagca gcgcggccgt gagaacggcg agcgcaagaa gactccccct gttcggagtt
     1021 cgatcggaca tcgttgtagt ttatgccagc atgacgacca caagcaacat tgccggggcg
     1081 tccgggcgcg ggcatcttgt tgaaacagac ggcggaggag gtcgacgatc cgatcctggt
     1141 tttcatattg cgtagcgtta tgcatttttg atataacgct acttgggacg cgacttcctt
     1201 cgttgggggg gctatgagcg ccagggaatc cagtctggag gtgcgctcca agatctggct
     1261 ggagatggac ggagagccgg tgttcggcca gggtcgggag gacctgctgc gccacatccg
     1321 gaggaccgga tcgatcaacg cggctgcgaa gaagatggga atcccgtacc ggaaggcgtg
     1381 gacctacatc gacgcgatgg agagacggat ggggtttccc ctggtgtcgc gcaagaaggg
     1441 cggggccggc gggggggaat cgacgcttac cccacaagcg atcatcctcc tggaaaaatt
     1501 ccactccctg aaaaagaact tcgacaaggg ggtggaccgg aagttcgccg aactggattt
     1561 cggaggatcg aggtgaatcc gtgcctgctc cccagctgat cctgcttgcg gtctgcatcc
     1621 tcgccgtggc ggtcctctac tccagcgtcg gacacggcgg tgcctcgggg tatctcgcgg
     1681 tccttgcatt gttcagcgta gcgcccgtcg ctttcaagcc gaccgcgttg atcctcaata
     1741 tccttgtctc cgcggtcgcc acggtccatt tcactcgcgc cggccatttc tcctggcgcc
     1801 tgttctggcc tttcgccgcc gcgtcgatcc ccttcagctt cgtcggcggc tacctcaccg
     1861 tgcccgcctc tgtctacaac cctcttctgg ggatcgtcct gctggcctct gcggttcgtc
     1921 ttttctcccg ggaggatacc gaacaggccg agtcgggccc gccttcgacc accgtggcgc
     1981 tcggggtcgg tgccgcactc ggtttcctct cggggctgat cggcgtcggg ggcggtatct
     2041 tcctgagccc cctcctgttg ctgatgaatt ggggcaacgc caaggaggcg tcagccgtcg
     2101 ccgcgctgtt catcttgatc aattccatct ccgggcttct cggtcatgtc agcagccttc
     2161 aggcgatacc gtccttcgtg ccggtcctgg ccgctacggc gttgtgtggg ggaatcaccg
     2221 gctctttctt tggaagtcgc cgcctgcaag ggacgagcat cgtgcggatc ctgtccgtcg
     2281 tgctgaccat cgctggaatc aagctcctgc tggtgtaagt tcgcggcgcg tggactcatc
     2341 actcgtcgtc ccgtaaggtc ttttccagtt cggtgaattc tttccggggc atcctcaccg
     2401 gaggcatcct cgcgaaggcc cttgcccgac gcggatcgtc gagccacttc cgtaaccgtt
     2461 ccaccgtcca cgcctttcct tctccggagg tccgggggta gaaccggctc agcaaccccg
     2521 agaggttcgg tcccgcgtcg cctttcccca gcccgccgag acgtcgtgtg agtacacggt
     2581 ggcaccctcc gcaccgcttg acgaagacgt tttcgcttcg ttcgtcgcgg tcgccgaacc
     2641 tcaccaccga agggacctcc cctttccggt ccggatcccg gcgagccccc gccacgagag
     2701 cattcaccac ttccgcggca ttttcctcca aaaaccggaa atcgggcatg aaggtcaccg
     2761 gttgaaggat ggaagcgaga attttattgg gattccggcc ggccgaccgg tcgaggtcgc
     2821 cggcgaggcg gtttccggtg ccgtgaatcg aatgacagcg ccggcagctt gcccggtcga
     2881 tccactgcgt gcccctttcc acggccgggt cgccggggaa gcgataacgg aggagacgac
     2941 cgggaacgag atcaaggtgc gcgatgtctt tccgaagggt ccggtcgttt ccaagatggc
     3001 aaccgatgca cgttccccgg tcggcaaaat ggaccggatg gcaggaactg cacgccggcg
     3061 gcatgccgtt cgcgggcgag gccacgacca gcacgatcgg gaggacgatg attagaaggg
     3121 acgtacgaag gtccactgcg gcccccgaaa aaccgccgcg atgacgctga gcgtgacgat
     3181 cgcgacgaag gtggcgagag cgaggaggtt caccgcccgc tgggtccgcg gcagccagga
     3241 tgccgccggg acagaaccgt acagcggcag ccagggcagg aacaggaaga ccagcccaag
     3301 aagaagtacc ggatagacga gggatgtgcc gtggcttacc acctcctgga tccaaaggag
     3361 gaaccaggcc gactttgccg ggttcggcgg ggccccggcg tccgccggtc ccagaagcgg
     3421 cgccggcaga aagatcgccc cggcgaggac gagagccgtg aacaggaggc cgcttcccgc
     3481 gatgaggcgg aagaagcggg gggaggactt gacgtagccg gcatcgggtt gccgcggtgg
     3541 tttcataggt agggaagcac tcccttgttt tttcgaacgc ggtaaaagtg gaggtacgaa
     3601 agggtgagca cccccatcgg caggatcgcg atatgcaaga cgtaaaaccg gaggagcgac
     3661 aggccccctc ccgtcccgtc cgggaccaga atgtcgtgga ttgccccccc gaagggcagc
     3721 gcccggagca attccatgcc ggtgcgggtc gcccagaagg ccagttgatc catggggagg
     3781 agatagccgg tgtagccggc gaagaccccg agccccagca gtccgcagcc gatcagccag
     3841 ttgaaatccc ggggtttctg gtaggcgccg gtcagcagca ccctcagcgt gtggaggaag
     3901 atcagcccca ggaagaggtg ggaggcgagc cggtgcaggc tgcggaggta gagccctccg
     3961 tacacttcgg actccaggaa gaggatggag tggtaggccc gctcagggga cggctggtag
     4021 taacaggcga ggaggatccc ggaagcgccc aggagaagga aggaggtgaa ggcaagcccc
     4081 cccaggcaga gcgtgtaccc gatggtcagg ttccgcgaaa ggaccacgcg ggggaagaga
     4141 tggcggatga attcccggga catgggttcc tttccttcag ctcgtcacga tcagccgctc
     4201 gccgcgcaca tcgaacggga ggagggggag agggcgggtg gccggcccct ccagcaccgc
     4261 cccccggcgg tcgaagcggc tgccgtggca gggacagagg attccggcag gtgtgacggc
     4321 gacggtgcat cccaagtggg tgcagaccag gctgatggcg tggaagccgt cgccgtcgcg
     4381 gaccaccgcc acccgcgcct cgcggaacac cagggcgccg tcgggcggaa gatccgcgag
     4441 ggcgacggcg agcaactcct tccggcgccg ggggcgaggc cggaggaagc ggtcgagtcc
     4501 cgccagcgcg ccggcgaatg cgatcagcgc gccgaggaat cgtcgacgtg atgtacgagg
     4561 atccgccatc tctcgaggta ccctctctga aagtccggcc cccgtgccga gaggcgccgg
     4621 tacgattccg ccggcaggaa ggcgccgttc accaccctct gaccggttcg gtcccagaac
     4681 gattccagcg tcatgccgtc ctcccggata cggtagatcc ggtccctcgg gtcttccatc
     4741 aggaagcgcc gcggggcgtc gtggcaaccg tcgcacagga ccgtgccgcg gcggatggtg
     4801 tgggggaaga aggccttcca ctcggccgcc agcaggcggt tctccgtccc ctcggccttg
     4861 tcgcgccgga gatcggtata gtacgcgatg aactggggcc ggatcgggct gatcttcccc
     4921 cgcccgttca tccccagcgg aggcgcgtcc tgcctgcgca ggtaggcgct ccgcaggtat
     4981 tcgctctccg tgtcgttccc cttcagccag aaatcctcgc gggaccggct ctccgtgatg
     5041 cgaaggaaga aggtcccgta ctcctgtgca gcccacgcgc tgtggcaggc gtagcactcc
     5101 aggcggtccc gatgggccgc gatccggtgc tccggcgagg atcccttcca ccgatgacag
     5161 tcggtgcacg ttttccctgc cttctttccc tcgacgaggc tccgcatggt gtggcaggcg
     5221 ccgcaggaaa gccctgcctc ggcgtggaca tcggggagca tggtgaggaa ggtctccccg
     5281 ttggcgaccc ttccgcgctg gtagcggaga ctgtcctcgc ggggcgcctt gccgaagtag
     5341 tcggcgccga cgtagtaccc tttgtggcag gcgaggcacc gggaagggga cgcccgggcg
     5401 gtgcggtgcc ctcccccctc atggcagtcg aggcagccgc tgatgtggca cgagccgcag
     5461 ttcttggcga agaagtcctt gtcgaccgcg gagaggcttc gcgccacgaa gtccttttcg
     5521 cggctgcggg tccccatgac gtgcccgaag atcgccgcgg tcccccggtg gcagc
//
LOCUS       DASVJL010000110         3783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1142842, whole genome shotgun sequence.
ACCESSION   DASVJL010000110 DASVJL010000000
VERSION     DASVJL010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142842"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VF357_02095"
     CDS             complement(<1..414)
                     /locus_tag="VF357_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522965.1"
                     /translation="MSGYNGLGMHVFFSIIRSFPGTFRNFVFLSVGVIDTSRFKGVAE
                     IENLSENLRGQLAKYVEFVKGYGYYADARYRVGTDVIEVLQGMAPEVVADFPNVVFFA
                     GQFVSQEENFFNKLLHNQTEFLAQKMLVFAGYPMIV"
     gene            complement(474..1661)
                     /locus_tag="VF357_02100"
     CDS             complement(474..1661)
                     /locus_tag="VF357_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX7522966.1"
                     /translation="MPSGSIYQRIKRILLGAPRDLLDPKIFHNVSLIAFFAWVGLGAD
                     GISSSSYGPEEAYLALGGHTVLALFVAAATVLTVFIISGSYAQIIEAFPSGGGGYIVA
                     SKLLGERAGVVSGSALVIDYVLTITISVAAGADAIFSFLPMPWLAYKFGFIVFILLFL
                     IWINLRGVKESVVALTPIFIAFLFTHIPLVLYAVFRHIGDLPAVATHVSGDLSAASRE
                     IGWLGVGALLLRAYSMGAGTYTGIEAVSNSMQTLREPRVQTGKRAMAYLAFSLAFMAG
                     GIILGYVLNGVAPVPGKTLNAVLFGGLVEGLWHGSGARPLIAFVLFTEAVLLFVAAQT
                     GFLGGPQVLSNMAVDSYMPHRFAHLSERLVTKYGVYFMGGMTFLMLFITGASTTCSSP
                     SPP"
     gene            complement(1755..3731)
                     /locus_tag="VF357_02105"
     CDS             complement(1755..3731)
                     /locus_tag="VF357_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX7522967.1"
                     /translation="MGNDSLYRRMKRVLLGAPRDLFDPKIFHNVSLIAFFAWVGLGAD
                     GISSSSYGPEEAYLALGQHSVLAIFVAVMTVATILIVSGSYSQIIEAFPSGGGGYIVA
                     SKLLGEKAGVVSGSALVIDYILTITMSVAAGTDAFFSILPPRYLHLKLGFVTAVIVIL
                     IWMNLRGIKESVAVFTPIFMLFMLGHIPLLLYAMGRHAADLPIVASEVSRDFQTTLGQ
                     VGWVGLAALLLRAFSMGGGTFTGIEAVSNSMQTLREPRVHTGKKAMLYMAVSLSFLAG
                     GILLCYMLYKVGHVPGKTLNAVLFEKVVGDLWQGTGGQILLAIVLGSETALLFVAAQT
                     GISDGPQVLSNMALDYYVPRRFAHLSERLVRNYGVVFMGGMALLMLYISGGSVQYLVI
                     MYSINVFLTFSLSQFGMILHWWKDRGTEKKWKFGLAMNGIGFLVTGTVLCVTVWFKFP
                     EGGWVTLLITALFVAGSLAIRHHYRSAQESMRRLDDLLLALPPVTVPAAQEPMVRRQA
                     STAVIMVSGYNGLGMHVFFSVVKQFPGMFRNFVFISAGVVDTSVFKGATEVENLGHNL
                     REQLEKYVEFVKGHGYYAEARVLIGTDAIESVTHLAGEVVKDFPNLCVFAGKLVFREE
                     NLLSRLLHNQTAFMAQKRLVFAGLPMIVLPIRVL"
BASE COUNT          766 a         1204 c         1245 g          568 t
ORIGIN      
        1 cacgatcatc gggtaccctg caaatacgag cattttctgc gcgaggaact ccgtctggtt
       61 gtggagcagc ttgttgaaga agttctcctc ctgggagacg aactgcccgg cgaagaagac
      121 gacgttgggg aagtcggcca cgacctcggg cgccatcccc tggagcacct cgatcacatc
      181 cgtgcccacg cggtaccgcg cgtccgcgta gtagccgtac cccttcacga actcgacgta
      241 tttcgcgagc tgcccccgca ggttttccga gaggttctcg atctccgcca cccctttgaa
      301 ccggctggtg tcgatcactc cgaccgacag gaacacgaag ttccggaacg tcccggggaa
      361 ggaccggatg atcgagaaaa aaacgtgcat cccgaggccg ttgtacccgg acacgaggat
      421 ggcggctgtc ggagcctgcc gccggacgat cggctcctgg gcggtcgccg tcgtcaggga
      481 ggggagggcg aggagcaggt cgtcgaggcg ccggtgatga agagcataag gaacgtcatc
      541 ccccccatga agtagacccc gtacttcgtc accagccgct cggagaggtg ggcaaaccgg
      601 tggggcatgt aggagtcgac cgccatgttg gagaggacct gcggcccgcc gaggaatccg
      661 gtctgggccg cgacgaacag cagcaccgcc tcggtgaaaa ggacgaaggc gatcagcggc
      721 cgggcgccgc tcccgtgcca gagaccctcc accagcccgc cgaagagaac ggcgttgagc
      781 gtcttcccgg ggacgggggc gacgccgttc aggacgtagc cgaggatgat cccccccgcc
      841 atgaaggcga gggagaaggc aaggtacgcc atcgcgcgct tccccgtctg cacgcgcggc
      901 tcccgcagcg tctgcatcga gttgctgacc gcctcgatcc cggtgtatgt gccggcgccc
      961 atcgagtacg cccgaagcag cagcgccccg acccccagcc agccgatctc ccgggaggcg
     1021 gcggagaggt cgccggacac gtgggtggcc acggcgggaa ggtcgccgat gtgccggaac
     1081 acggcgtaga ggaccagcgg gatatgggtg aacaggaagg cgatgaagat cggcgtgagg
     1141 gcgacgaccg actccttgac cccccgcagg ttgatccaga tgaggaagag aaggatgaag
     1201 acgatgaacc cgaacttgta ggcgagccac ggcatcggaa ggaagctgaa gatggcgtcc
     1261 gcccccgccg ccaccgagat cgtgatcgtc aggacgtagt cgatgaccag cgccgagccc
     1321 gagaccactc ccgctctctc cccgaggagc ttcgacgcga cgatgtaccc gccgccgccg
     1381 gaggggaacg cttcgatgat ctgcgcgtag ctccccgaga tgatgaagac ggtgagcacc
     1441 gtggccgccg cgacgaagag ggcgaggacg gtgtgccccc cgagggcgag gtacgcctcc
     1501 tccgggccgt acgaggagga ggagatcccg tcggctccca gccccaccca ggcgaagaag
     1561 gcgatcaggg aaacgttgtg gaagatcttc gggtcgagga ggtcccgggg agcgcccagc
     1621 aggatcctct tgatgcgttg atagatggat ccgctcggca tcggtcgcgc cggtctccgt
     1681 tatcctgata cggggctttt gccgggcgaa ttataccatc ggccgctgga tgcgcacgca
     1741 gaaaccgccc tgtgctacag cacgcggatg ggaagaacga tcatcggcag gcccgcgaac
     1801 accagccgtt tctgcgccat gaacgcggtc tggttgtgga ggaggcggga gagcaggttt
     1861 tcctccctga agacgagttt gccggcgaag acacagaggt tggggaagtc cttcaccacc
     1921 tccccggcaa ggtgggtgac ggactcgatc gcatccgtcc ctatcaagac tcgggcctcc
     1981 gcgtagtagc cgtgcccctt cacgaactcc acgtacttct cgagctgctc ccggagattg
     2041 tgccccaggt tctcgacctc cgtcgccccc ttgaacacgc tcgtgtcgac gacccccgcg
     2101 gagatgaaga cgaagttccg gaacatcccg gggaactgct tgacgacgga gaagaagacg
     2161 tgcatcccca ggccgttgta cccggagacc atgatgaccg ccgtggaggc ctgccgccga
     2221 accatcggtt cctgcgccgc ggggaccgtc accgggggaa gcgcgaggag caggtcgtcc
     2281 aggcgccgca tgctctcctg ggcgctccgg tagtggtgcc ggatggcaag actccccgcg
     2341 acgaacaggg cggtgatgag gagggtcacc cagccgccct ccgggaactt gaaccagacg
     2401 gtgacgcaga ggaccgtccc cgtcacaagg aacccgatgc cgttcatggc caggccgaat
     2461 ttccatttct tctccgtccc cctgtccttc caccagtgca ggatcatgcc gaactgggag
     2521 agggagaagg tgagaaagac gttgatcgag tacatgatga cgaggtactg gaccgacccg
     2581 ccggagatat aaagcatcag aagcgccatc ccgcccatga acaccacgcc atagttgcgg
     2641 accaaccgct cggagaggtg cgcgaagcgg cgggggacgt agtaatccag cgccatgttg
     2701 gagaggacct gcgggccgtc gctgatgccg gtctgggccg caacgaacag gagggccgtc
     2761 tccgacccga gcacgatcgc caggaggatc tggccgccgg tcccctgcca gaggtccccg
     2821 acgaccttct cgaagaggac ggcgttcagc gtcttccccg ggacgtgccc gaccttgtac
     2881 agcatgtagc acagaaggat cccgcccgcg aggaacgaca gggagaccgc catgtagagc
     2941 atcgccttct tccccgtgtg gacgcggggc tcccgcagcg tctgcatcga gttcgacacc
     3001 gcctcgatcc cggtgaaggt gccgccgccc atcgagaaag cgcgcagcag cagggcggcc
     3061 agccccaccc atccgacctg tccaagagtg gtctggaaat ccctggacac ctcggacgcg
     3121 acgatcggca ggtccgccgc gtggcgcccc atcgcgtaca ggagcaacgg gatgtgcccg
     3181 agcatgaaca gcatgaagat gggggtgaag acggcgacgg attccttgat cccccgcagg
     3241 ttcatccaga tgaggatgac gatcaccgcg gtgacgaatc cgagcttgag gtgcagatac
     3301 cgtggcggaa ggatgctgaa gaaggcgtct gtccccgccg cgacggacat ggtgatcgtc
     3361 aggatgtagt cgatgaccag cgccgacccg gagaccaccc ccgccttctc cccaagaagc
     3421 ttcgacgcaa cgatgtaccc gccgccgccg gaagggaacg cctcgatgat ctgtgagtaa
     3481 ctgccggaga cgatcaggat cgtcgccacc gtcatcaccg cgacgaagat ggcaagcacc
     3541 gagtgctgcc cgagggcgag gtacgcctcc tccgggccgt acgaggagga ggagatcccg
     3601 tccgcgccca gccccaccca ggcgaagaag gcgatgaggg agacgttgtg gaagatcttc
     3661 gggtcgaaga gatcccgggg ggccccgagc agaacccgct tcatccgccg gtacagagaa
     3721 tcgtttccca tcggtgtcgt catcccctac tcctcttccc ggaacgggac ttcgacgacg
     3781 tcg
//
LOCUS       DASVJL010000111         5174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_204474, whole genome shotgun sequence.
ACCESSION   DASVJL010000111 DASVJL010000000
VERSION     DASVJL010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204474"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            469..795
                     /locus_tag="VF357_02110"
     CDS             469..795
                     /locus_tag="VF357_02110"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7522968.1"
                     /translation="MHGFWNPENIALKVLAPGVTAKIASGEKMMFSLVTLAPDAVVPA
                     HSHPHEQMGLMVSGTMVFTIEGETRVLSGNEMYFVPGGVSHAAKGGPGGAVALDAFSP
                     PREEYR"
     gene            complement(792..2369)
                     /locus_tag="VF357_02115"
     CDS             complement(792..2369)
                     /locus_tag="VF357_02115"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX7522969.1"
                     /translation="MADKVFIFDTTLRDGEQVPGAKLARDQKVEIARQLAVLNVDIIE
                     AGFPASSPGDFESVQAVAKTVRGPVITGLARAVKKDIDTLWDAVKVAKRPRIHTFLGT
                     SDIHIQKKFRADREKILQWCVDTVKYARSLCRDVEFSTEDASRTDFEYLCRTVDAVVK
                     AGATTINIPDTVGYATPMEMADRVRRLKEKVPGLDHAILSMHCHDDLGMSTANTLAGI
                     LAGARQAEVTVNGIGERAGNASLEEVVLAIKTRKDIFEGLTTGVNTKEIAKTSRMVSK
                     LMGLPIQRNKAIVGTNAFAHSSGIHQDGIIKDRSTYEIIRPEDVGVTAHTFTLTARSG
                     RAALKHHITGMGHRLTDAQLAEIYEKFLVLADKKKEVMVEDLEILVQDELFKVPETYK
                     LINLQILSGTKATPMAALKVQRNNEVIEEAATGNGPIDAAYKCIERIVGANFKLIDFG
                     LNAVTSGKDAIGEARVRIRSNGTIFSGGASSTDIIEAAIKAYLSAINRWVAAEAKTKA
                     GKKGAKKKGAVAPIQVP"
     gene            complement(2649..3260)
                     /locus_tag="VF357_02120"
     CDS             complement(2649..3260)
                     /locus_tag="VF357_02120"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX7522970.1"
                     /translation="MYGNVKKSFLTGILILVPLVVSVTLLLWFFQKVDDLFSPVLDGV
                     FRTLIPGTGHVPGTGILAGLVIILLLGTLARNVAGKRLLDALDRLIHRIPGYRTVYST
                     IQQLTNAFSPENTQSFKEVLLVEYPLEGSYALGFRTMTVKEGERRLVVVYIPTNHLYL
                     GEVLFIPEEKAVRLDMSIEQAVRLLVSGGIAAPRELRRMQGGA"
     gene            3302..4363
                     /locus_tag="VF357_02125"
     CDS             3302..4363
                     /locus_tag="VF357_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7522971.1"
                     /translation="MDHAVVMAGGSGTRFWPESRAKRSKQFLDLTGGGPMIRETLLRL
                     FPVIPPERVWVVAGVKDAPHLSHRDLGIPKRHILLEPEGKNTAPAMAYAAAAVAREDP
                     DAVILATPADHAIGHVRAFQAVLRKGLRLARETGRFVTLGVPPTKPSTGYGYIERGKP
                     FPGKVRGAFEVARFAEKPDLPTAKRFLRLGRFDWNSGLFLFGVVTFADRLAKFLPEVD
                     REIRRAFRGDRTGFPKRLARAYRRMPSISVDYGILEKEKGILVLPANVGWSDLGTWHS
                     LHEFLAERGENVTFGDVILSNCRSVLVRTDCGVAAVLGMDDVVVVRSGDAVLVCPRVR
                     SEEVKGVLEEVRRRFPALA"
     gene            complement(4364..4840)
                     /gene="greA"
                     /locus_tag="VF357_02130"
     CDS             complement(4364..4840)
                     /gene="greA"
                     /locus_tag="VF357_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX7522972.1"
                     /translation="MLREVKKKLEEEMQRIEHELRVTLPKEIQTALGQGDLSENAEYE
                     SAKNRQSTIQARFAQIQKRLADLSRIDVSGVPKDRAGLGSEVTVENLESGEEIRYTLV
                     IPELADGNKSFVSMASPVGKALMNRRVGDTVTITIPRGTLEYEVRRVVTLSGDVLE"
     gene            complement(4854..>5174)
                     /locus_tag="VF357_02135"
     CDS             complement(4854..>5174)
                     /locus_tag="VF357_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX7522973.1"
                     /translation="WVIERADLVEAMSEQISQSSLLEKFDAKKLAKADTTQILKAAID
                     REIEANRFYLDMAGRSKKDSAKEMFLSLAKEEELHAKILRAEVDSIGQNGFWCDLQEF
                     TMEQ"
BASE COUNT          838 a         1718 c         1620 g          998 t
ORIGIN      
        1 cccggagtgc ttcaattcat aaaaacggta atacgatcgt gggaactcag actccgggct
       61 cgtgcccccc gttattcctg ctgcgggggt accccaccag cgcgaagctc gtagtggggc
      121 gggggggctt cctctccgct acggctcgtc gtccctccct ggattcctcg cctgcgcttc
      181 cgctcgttct cgacgtccct gtctccactt cgctccagac gccgctccga ggaagccccc
      241 cgctgcgtca ctcctgcgca caggattctc cgcctccatc gccgtacctt atgtgggatc
      301 ccctcccgag cgggcatctg accaggccgg agccaggacg gcgagaggac ggggaggtgc
      361 cagcgaaccg gacagggacg tccggtgagc gtagcgaggg agggtgatcc cacggaaggg
      421 aaggcagaat ccgatgaatg atgcatttat tccaacagga ggtgtcccgt gcacggtttc
      481 tggaaccctg agaacatcgc actaaaggtg ctggcgccgg gggtgacggc gaagatcgcc
      541 tcgggggaga agatgatgtt ctccctcgtc acccttgctc cggatgcggt cgttccggcg
      601 cactcccatc cgcacgagca gatgggattg atggtgtcgg gaacgatggt gttcacgatc
      661 gaaggcgaga caagggtgct gtcgggaaac gagatgtact tcgttccggg tggggtttcg
      721 cacgcggcga aggggggtcc cgggggggcg gtggcgctgg acgccttctc gcccccccgg
      781 gaggagtacc gttaggggac ctggatcggc gccactgcgc cttttttctt ggcgcccttt
      841 ttcccggcct tcgtcttcgc ctccgcggcc acccaccggt tgatcgcgga gaggtacgcc
      901 ttgatcgccg cctcgatgat gtcggtggag ctcgcgccgc cggagaagat cgtcccgttg
      961 gagcggatcc gcacccgcgc ctccccgatc gcgtcctttc ccgacgtgac cgcgttcagc
     1021 ccgaaatcga tcagcttgaa attcgccccg acgatccgtt cgatgcactt gtacgcggcg
     1081 tcgatcggcc cgttccccgt cgccgcctcc tcgatcacct cgttgttccg ctgtaccttc
     1141 agcgccgcca tcggcgtcgc cttcgtcccc gacaggatct ggaggttgat cagcttgtac
     1201 gtctccggga ccttgaacag ctcgtcctgc acgaggatct cgaggtcctc gaccatgacc
     1261 tccttcttct tgtcggccag gacgaggaac ttctcgtaga tctcggccaa ctgggcatcg
     1321 gtcaggcggt gccccatccc ggtgatgtgg tgcttgagtg ccgcgcggcc cgagcgggca
     1381 gtgagcgtga acgtgtgggc cgtcactccc acgtcctccg gccggatgat ctcgtacgtg
     1441 gagcggtcct tgatgattcc gtcctggtgg atgccggagg aatgggcgaa ggcgttcgtc
     1501 cccacgatcg ccttgttccg ctggatcggc agccccatca gcttggacac catccggctc
     1561 gttttcgcga tctccttcgt gttcacgccg gtggtcaacc cctcgaagat gtccttgcgc
     1621 gtcttgatcg ccagcaccac ctcttcgagc gacgcgtttc ccgcgcgctc gccgatcccg
     1681 ttcaccgtca cctcggcctg gcgggcgccg gcgagtattc ccgcgagcgt gttcgccgtg
     1741 gacatcccga ggtcgtcgtg gcaatgcatc gagaggattg cgtggtccag cccgggaacc
     1801 ttctccttga ggcgccgcac ccggtccgcc atctccatcg gtgtggcgta cccgacggtg
     1861 tccggtatgt tgatcgtcgt cgcgcccgct ttcacgaccg catcgacggt ccggcagagg
     1921 tactcgaagt cggtccgcga tgcgtcctcg gtggagaact cgacgtcccg acagagggaa
     1981 cgggcgtact tcaccgtgtc cacgcaccac tgcaagatct tctcccggtc ggcgcggaac
     2041 ttcttctgga tgtggatgtc cgaggtgccg aggaacgtgt ggatccgggg gcgcttcgcc
     2101 actttcaccg cgtcccacag tgtatcgatg tcctttttca ccgcccgcgc gaggccggtg
     2161 atgaccggtc ccctgacggt tttcgcgacc gcctggaccg actcgaagtc gccgggggag
     2221 gaggccggga accccgcctc gatgatgtcc acgttcagca ccgccagctg ccgcgctatc
     2281 tcgaccttct ggtccctggc gagcttcgcc cccgggacct gttccccgtc ccgcaacgtc
     2341 gtgtcgaaga tgaaaacctt gtccgccatc gaatcctcct ttactcttcc ggtttgtggg
     2401 cgggggacac tcctccccaa aattaaaaac ccccggtgac ttgtggtccc cgggggctgg
     2461 aaagaaaaaa ccgcgggaac ctgccggccc cgcggcccga atcgacgtaa atggtgcgct
     2521 acgcgctcag gctgcggtcc ggcccctcca ggggcgaagt agagcgagcc cgagcaaccc
     2581 aatttcctgc ggatatccga tcgcgctcat catgattcga ttagaatacg gggccgtcga
     2641 tctccttgtc aagccccgcc ctgcattctc cgcagctccc gcggcgccgc gatccccccc
     2701 gagacgagca ggcggaccgc ctgttcgatc gacatatcga gccggaccgc tttctcctcg
     2761 gggataaaaa gcacttcgcc cagataaaga tggttcgtcg gaatgtacac gacgaccagc
     2821 cggcgttccc cctccttcac cgtcatcgtc cggaacccga gggcgtacga cccctccagc
     2881 gggtactcca ccaggaggac ctccttgaac gattgcgtat tctcggggga gaaggcgttc
     2941 gtgagttgct ggatggtcga gtagacggtt cgatagccgg gaatccgatg gatcagccgg
     3001 tcgagggcgt cgaggagccg cttccccgcc acgttccggg ccagcgtacc caacagcagg
     3061 atgatgacga gcccggccag gatccccgtg cccggaacgt gacccgtgcc tgggatcagc
     3121 gtccggaaaa ccccgtcgag caccggagaa aagagatcgt cgaccttctg gaagaaccag
     3181 aggaggagcg tcaccgacac cacgaggggg acgaggatca agatcccggt gaggaaactt
     3241 tttttcacgt tgccgtacac gagggtattc tatcgtaaat gcttcccggg agggcgttca
     3301 catggatcac gcggtggtga tggcgggcgg gtcggggacg aggttctggc cggagagccg
     3361 tgcgaagcgc tcgaagcagt ttctggacct caccggcggg ggaccgatga tccgggagac
     3421 cctcctgcgt ctcttccccg tcatcccccc ggaacgcgtc tgggtcgtcg ctggcgtcaa
     3481 ggatgcgccg cacctctccc accgcgacct cggcatcccg aaacggcaca tcctcctgga
     3541 gccggagggg aaaaacaccg cccccgcgat ggcgtacgcg gcggcggccg tcgcccggga
     3601 ggaccccgac gccgtgatcc tggccacccc ggccgaccac gccatcggcc acgtccgggc
     3661 gttccaggcc gtgctccgga aggggcttcg cctggcgcgg gagacggggc ggttcgtaac
     3721 cctcggcgtc ccgccgacca aaccctccac gggatacggg tacatcgagc gggggaaacc
     3781 gttccccgga aaggtccggg gcgcattcga ggtcgcccgg ttcgccgaaa aacccgacct
     3841 tccaacggcg aagcggttcc tccgcttggg ccggttcgac tggaacagcg ggctgttcct
     3901 attcggcgtc gtgacgttcg ccgaccgtct ggcgaaattc ctccccgagg tcgatcgcga
     3961 gatccgtcgc gcattccggg gggaccggac cggttttccg aagcggctcg cacgggcata
     4021 ccgtcggatg ccgtcgatct cggtcgacta cggcatcctg gagaaggaga aggggatcct
     4081 cgtcctcccg gcgaacgtcg ggtggagcga cctcgggacg tggcactcgc ttcacgagtt
     4141 cctcgcggag aggggggaaa acgtcacgtt cggcgacgtc atcctttcca actgccggag
     4201 cgttcttgtg cggacggact gcggcgtggc ggcggtgctg gggatggacg acgtggtggt
     4261 ggtccgaagc ggggacgccg tgctggtttg cccgcgcgtc cgctcggaag aagtgaaggg
     4321 tgtgctggag gaggtgcggc ggcggttccc cgcgctggcg tgactattcg agaacgtccc
     4381 ctgacagcgt gacgacccgc cggacctcgt actccagcgt cccgcgggga atcgtgatcg
     4441 tgaccgtgtc gcccacgcgg cgattcatga gcgccttccc caccggcgac gccatggaga
     4501 cgaacgactt gttcccgtcg gccaactccg ggatcacgag ggtgtaacgg atctcttctc
     4561 cgctctcgag gttttccacg gtgacctcgc tcccgagccc cgcgcggtcc ttcggcacgc
     4621 ccgacacgtc gatgcgggaa aggtcggcca ggcgcttctg gatctgggcg aagcgcgcct
     4681 gaatcgtcga ctgccggttc ttggccgact catactcggc gttctcggag aggtcgccct
     4741 ggcccaacgc cgtctggatc tccttgggaa gggtcactcg cagctcgtgt tcgatccgct
     4801 gcatttcctc ctcgagcttc ttcttcacct cgcgaagcaa cgcgatcccc tacctactgt
     4861 tccatcgtga attcctgcag gtcgcaccag aacccgttct gcccgatcga gtccacctcg
     4921 gcccgcagga tcttcgcgtg cagttcctct tccttcgcca gggagaggaa catctccttc
     4981 gccgaatcct tcttcgagcg tcccgccata tcgaggtaaa accggttcgc ctcgatctcc
     5041 cggtcgatcg cggccttgag gatctgtgtc gtatcggcct tggccagttt cttcgcatcg
     5101 aacttttcga gcaacgatga ctgcgagatc tgctcggaca tcgcctcgac cagatccgcc
     5161 cgctcgatca ccca
//
LOCUS       DASVJL010000112         5772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_204517, whole genome shotgun sequence.
ACCESSION   DASVJL010000112 DASVJL010000000
VERSION     DASVJL010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204517"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VF357_02140"
     CDS             complement(<1..590)
                     /locus_tag="VF357_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7522974.1"
                     /translation="MNGTKSVLSVLLLLPFLAACTGGGPGSAQAPSSVASVQDALVAA
                     VERSVPAVVNIRTVTRFARGTPGSGRRPDGQPPEYLVDLLEENGGFRENSLGSGVIIG
                     EDGLIVTNEHVIRDADEIVVRLSDRSEHRAKVVGADVRTDVAVLRIQPAGKLPVATLG
                     DSSRLKVGEFAIAVGNPFGLESTVTLGVVSATGRSA"
     gene            complement(587..1564)
                     /locus_tag="VF357_02145"
     CDS             complement(587..1564)
                     /locus_tag="VF357_02145"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX7522975.1"
                     /translation="MDVIEGSGNFSPPGETSLTIGNFDGVHLGHRELLRRTVAHARDL
                     RLLAVALTFTPHPVRFFTPKARFYEITSMGEKASRMEKLGIDCLVVETFNGAVGGMGP
                     EEFARTVVHGRLHARFVTVGYDFTFGRNRTGSPGMLRRIGSELGFEVDIVPPLMRGGA
                     IVSSSRIRELLLSGRVREAEELLCRPYAVSGRVIPGAARGRKLGFPTANVEFVQELLP
                     LPGVYVIDAVVGGVVRRGVANVGFNPTFGENSLGVEAHLLDFAGDLYRQDMTVRFRDR
                     IRDERKFKSVEELVRQIEKDVGYARSARLPTDPETDDAAFGGRLADDGQ"
     gene            1636..2769
                     /locus_tag="VF357_02150"
     CDS             1636..2769
                     /locus_tag="VF357_02150"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX7522976.1"
                     /translation="MRITRKYLLGEMTGPFLVGVGSFTVIVLLQQFSRLADLVIAKGV
                     PTPLVGRLLLSLIPPFFEITLPASLLLAVLLGLGRLAADSETTALSAAGVGMLGVALP
                     VLVASVITCAAVLLTGWHGVPWGYRETQRTLARIVSERAGAGASGHVFREITPEVLVY
                     PDRVSPDGQRMSGVFLSFRPTGDDPLLVFAHEGRFVPATGDGVVGLELSDGTIHGESP
                     GKPVYRVASFGRMTFRIPLEASSILGGNDPKGMTLPELSRKIDATGGRGANATYRYHF
                     HRRLSLAVSCISFGLLAIPLGFSLRSRGKSSAVGIAVALFLIYYLFIAAAGAVEKRSG
                     PGMIALLWAPHALGLSLAAWILWRSEHCVTLFPRIFGRDRLRK"
     gene            2766..3842
                     /gene="lptG"
                     /locus_tag="VF357_02155"
     CDS             2766..3842
                     /gene="lptG"
                     /locus_tag="VF357_02155"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX7522977.1"
                     /translation="MTVLSRYLFREFLRAAAACILGFLLLFLLIDFVDHAEKLLQHNA
                     GLREIGWYYLTRVPGIFVLISPVGTMLAVLVAVSLRVRSNELTAIFSSGVSLLRVCVP
                     ILVGCALVSALSLLSSEVLAPAANRHAREIERLRVRPGKVAAQFSGNRYWMRGERGIL
                     SAQVVDGPSRSLRGFQYIEVGPDFLPLRRIESRKARLLSDGAWELADGRERIFGETPS
                     VAAFSLRTYRFPETMDGFIEGETPPEEMTYAQLSGYVGELRRKGFEARGYETDLYAKI
                     AYPLLNVLISMLAIPFALRSPRSGGVWRSIGLGLLMGFTCWVVLSASLSLGRKGMLPA
                     LLAAWLPGVLFAGSGAALFRGVGR"
     gene            complement(3847..5115)
                     /gene="ahcY"
                     /locus_tag="VF357_02160"
     CDS             complement(3847..5115)
                     /gene="ahcY"
                     /locus_tag="VF357_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX7522978.1"
                     /translation="MGPMKGHDVKDLKLAAAGKSRVEWAKKDMPVLRSIGVRFAKEKP
                     LKGVRIAACLHVTTETAALMQVLAAGGAKVALCASNPLSTQDEAAASLVKNDGIAVYA
                     IKGENRKTYYRHILAALAVGPNMTMDDGADLVSMVHSEKKEYLKEIVGGTEETTTGVI
                     RLSAMGEKGVLRYPIIAVNEAKTKHFFDNRYGTGQSTIDGILRATNRLLAGSCFVVAG
                     YGWCGRGLAMRARGMGARVIVTEIDPLPALEALMDGFEVEPMAEAARVGDFFCTVTGN
                     LHVVRREHFERMKDGAIVCNSGHFNVEIDIPALAKMAKSVRTVRPYVEEYVLGDGRAI
                     HVLGEGRLINLAAAEGHPANVMDMSFANQALSAEYMARNHGALSNTVHLVPEAIDREI
                     ARLKLASSGARIDRLTPEQRDYLASWEMGT"
     gene            complement(5336..>5772)
                     /locus_tag="VF357_02165"
     CDS             complement(5336..>5772)
                     /locus_tag="VF357_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HEX7522979.1"
                     /translation="GRKIIVDTYGGYSRHGGGAFSGKDPSKVDRSAAYMARYVAKNVV
                     AAGLARKCEVQLAYAIGVAEPVSVLVETFGTGNVPEERIGRAVMDTFDLRPARIIREL
                     NLLRPIYRKTASLGHFGRELPEFTWEKTDKVRALRKAAGHGA"
BASE COUNT          843 a         2052 c         1817 g         1060 t
ORIGIN      
        1 tccgcgctgc gcccggtcgc gctcaccacg ccgagggtca cggtgctctc gaggccgaac
       61 gggttcccca ccgcgatggc gaactccccg accttcaggc gggaggagtc gcccagcgtc
      121 gccaccggaa gctttccggc cggctggatc cgaagcacgg ccacgtccgt ccggacgtcc
      181 gcgccaacca ccttcgcccg gtgctcgctc cgatccgaaa gacggaccac gatctcgtcc
      241 gcgtcgcgga tgacgtgctc gttggtgacg atcagcccgt cttccccgat gatgaccccc
      301 gacccgaggg agttctcgcg gaatccgccg ttctcctcga gcaggtccac gagatactcc
      361 ggcggctggc cgtccggccg ccgccccgac cccggcgttc cgcgcgcgaa acgggtgacc
      421 gtgcggatgt tgaccaccgc ggggacggaa cgctccacgg cggcgacgag cgcatcctgg
      481 accgaggcga cgctggacgg cgcctgcgcg ctccccggcc cccccccggt gcacgcggcc
      541 aagaacggga ggaggagaag gacggagaga accgactttg ttccgttcat tggccgtcgt
      601 ccgccagccg tccaccgaag gccgcgtcgt ccgtttccgg atccgtcggc agccgtgcgg
      661 atcgcgcata cccgacatcc ttctcgatct ggcgcaccag ttcctccacg ctcttgaact
      721 tccgctcgtc tcggatgcgg tccctgaagc gcaccgtcat atcctgcctg tagagatcgc
      781 ccgcgaagtc gaggaggtgc gcctccaccc cgagagagtt ctctccgaag gtggggttga
      841 acccgacgtt ggccacccct ctgcgcacga cacccccaac gacggcatcg atgacgtata
      901 ctcccggcag cgggaggagt tcctgtacga actcgacgtt tgccgtcgga aacccgagct
      961 ttcgtccccg ggctgcgccg gggatcaccc tgccggaaac cgcatacggg cggcacagca
     1021 gttcctcggc ttcccgcacc cgccccgata gcaggagctc ccggatacga ctggagctca
     1081 cgatcgcgcc cccgcgcatg agcggcggga cgatatccac ctcgaacccg agctcgctcc
     1141 cgatccggcg gagcatcccg ggagaccccg tccggttcct tccgaaggtg aagtcgtatc
     1201 ccaccgtgac gaaacgggcg tgcagccgcc catggacgac cgtccgggcg aactcctccg
     1261 gtcccatccc gccgaccgcc ccgttgaacg tttcgacgac caggcaatcg atcccgagct
     1321 tctccatccg ggacgccttt tcccccatcg aggtgatctc gtagaagcgc gccttggggg
     1381 tgaagaagcg gaccggatgg ggcgtgaagg tgagcgcaac ggccagcagg cgtagatccc
     1441 gtgcgtgcgc cacggtccgc cgcaaaagct cccgatggcc aagatgcacc ccgtcgaaat
     1501 ttccgatcgt cagggaagtc tccccgggag gggagaagtt cccggatccc tcgatgacat
     1561 ccattcggcc cgaccgtctc ctcctcccgg ttccgggctc ctccggcggg gaatgataaa
     1621 gtatgatact acacgatgag aatcacacgg aaatatctgc tcggagaaat gaccggcccg
     1681 ttcctggtcg gtgtgggaag cttcaccgtc atcgtcctgc tccagcaatt ttcgcggctc
     1741 gcggacctgg tgatcgcgaa gggggtgccg acgccccttg tcgggcggct cctcctctcc
     1801 ctcatccctc cgttcttcga gatcaccctt cccgcttccc tgctcctcgc ggtcctgctc
     1861 ggcctggggc ggctcgccgc ggactcggag acgacggcgc tctccgccgc cggcgtgggg
     1921 atgcttggag tcgccctccc cgtgctggtc gccagcgtca tcacttgcgc cgcggtgctg
     1981 ctcaccggat ggcacggggt cccctggggg taccgcgaga cgcagcgcac cctcgcccgg
     2041 atcgtttccg agcgcgccgg cgccggtgcc tccggccacg ttttccgcga gatcaccccc
     2101 gaggtcctgg tgtaccccga ccgggtctcc ccggacgggc agcggatgtc cggcgtcttt
     2161 ctctccttcc gtccgacggg agacgatccg cttctcgtgt tcgcgcacga ggggcgcttc
     2221 gtacccgcca ccggcgacgg tgtggtcggc ctggaattgt ccgacgggac gatccacggc
     2281 gagtcgccgg gaaagccggt gtatcgcgtc gcctccttcg gccggatgac gttccggatc
     2341 cccctcgagg cctcctccat tctcggaggg aacgacccga aagggatgac cctccccgaa
     2401 ctgtcacgga agatcgacgc gaccgggggc agaggagcga acgccaccta ccggtaccat
     2461 tttcaccgcc ggctctccct tgccgtaagc tgcatctcct tcggtctgct cgcgattcct
     2521 ctcgggttct ccctgcgatc tcgtggaaaa tcgtccgccg tcgggatcgc ggtcgccctg
     2581 ttcctgatct actacctgtt catcgccgcg gcgggagcgg tggagaaacg ctccggtccc
     2641 gggatgatcg cccttctctg ggcgccacac gccctcgggc tctccctcgc cgcgtggatc
     2701 ctctggcggt cggagcactg tgtgaccctc ttcccccgca tcttcggcag ggatcggctc
     2761 cgcaaatgac cgtcctctcg cgctacctgt tccgggagtt cctccgcgcc gcggccgcct
     2821 gcatcctcgg cttcctcctc ctgttcctgc tgatcgactt cgtcgaccac gccgaaaaat
     2881 tgctgcagca caacgcgggt ctccgggaga tcgggtggta ctacctcacc cgcgtgccgg
     2941 gcattttcgt gctgatctcc ccggtcggga cgatgctggc cgtgctggtc gccgtctccc
     3001 tgcgcgtacg ctcgaacgag ctgacggcga tcttctccag cggggtgagc cttctccgcg
     3061 tctgcgtgcc gatcctggtc gggtgcgccc tggtgtctgc cctgtcgctg ctctcctccg
     3121 aggtgttggc accggccgcg aaccggcacg cccgggagat cgagcgcctc cgcgtgcggc
     3181 cgggaaaggt ggcggcgcaa ttctcgggga accggtactg gatgcgcggc gagcggggga
     3241 ttctctccgc ccaggtggtg gacggtccgt cccggtcgct gcgggggttc cagtacatcg
     3301 aggtgggccc ggatttcctc ccgcttcgca gaatcgagtc ccggaaggcc cgcctccttt
     3361 ccgatggcgc gtgggagctt gcggacggga gggagcggat cttcggagaa acgccatcgg
     3421 tggcggcgtt ttccctccgg acgtaccggt tcccggaaac gatggacggg ttcatcgagg
     3481 gggaaacccc ccccgaggag atgacctacg cgcagctctc gggatatgtc ggggaactga
     3541 ggcggaaggg gttcgaggcg agaggctacg aaaccgacct gtatgcgaag atcgcctacc
     3601 ccctgctgaa cgtcctcatc agcatgctgg cgatcccctt cgccctgcgc tcgccccggt
     3661 cgggcggcgt ctggcgcagc atcgggctcg gtctcctgat ggggttcacc tgctgggtcg
     3721 tcctgtccgc gtccctctcg ctgggcagga aggggatgct cccggccctt ctcgccgcct
     3781 ggctccccgg ggtcctcttc gccggctccg gagccgcgct gttccgcggc gtgggtcggt
     3841 agtagctcag gtccccattt cccaggaggc gaggtagtcg cgctgctccg gcgtgaggcg
     3901 gtcgatccgg gcgccggagg acgcgagctt gagccgcgcg atctcccggt cgatcgcctc
     3961 ggggaccagg tggacggtgt tcgacagcgc cccgtggttc cgggccatgt actccgccga
     4021 aagcgcctgg tttgcgaagc tcatgtccat cacgttcgcc gggtgccctt ccgcggccgc
     4081 caggttgatc agccgcccct ctccgaggac atggatcgcg cggccgtccc ccagcacgta
     4141 ctcctcgaca tacggcctga ccgtgcgcac cgacttcgcc atcttcgcca gcgcggggat
     4201 gtcgatctcg acgttgaagt ggccggagtt gcatacgatc gccccgtcct tcatccgctc
     4261 gaaatgctcc ctgcgaacca cgtgcaggtt tccggtgacg gtgcagaaga agtccccgac
     4321 cctcgcggcc tcggccatcg gctccacctc gaagccgtcc atcagcgcct cgagcgccgg
     4381 aaggggatcg atctcggtga cgatcacccg ggcgcccatt ccccgggccc gcatcgccag
     4441 tccccgtccg caccagccgt agccggccac gacgaaacag ctccccgcca gcagccggtt
     4501 cgtcgccctg aggatgccgt cgatcgtcga ctgccccgtt ccgtaccggt tatcgaagaa
     4561 gtgcttcgtc ttcgcctcgt tcaccgcgat gatggggtac cgcaggaccc ccttctctcc
     4621 catcgcgctc aaccggatga cgcccgtggt cgtttcctcg gtgcccccga cgatctcctt
     4681 caggtactcc ttcttctccg agtggaccat cgagaccagg tccgccccgt cgtccatcgt
     4741 catgttcggt cccacggcga gggccgcgag aatgtgccgg tagtacgtct tccggttctc
     4801 ccccttgatt gcgtagacgg cgatgccgtc gttcttgacg agggaggcgg cggcctcgtc
     4861 ctgcgtcgaa aggggattgg aggcgcacag ggcgaccttc gccccgcccg ccgccagcac
     4921 ctgcatgagc gccgcggtct ccgtggtcac atggaggcag gcggcgatcc ggacgccttt
     4981 gagcggcttc tctttcgcga accggacccc gatggagcga agcaccggca tgtccttctt
     5041 tgcccactcg acgcggctct tccccgcggc ggcgagcttc agatccttga cgtcgtgccc
     5101 tttcatggga cccataggtt aacccctttc gcttgtatgc acactcggcg cattgtgctt
     5161 cgtctcacaa ttgctctccc aacaacaggg gcgcatcgga gcgggatact ccttccgagc
     5221 gggcgtgtgt tttttccgga ggcaggaagg gctgccggaa cgtagcgagg aagggggtat
     5281 cacgcgaaga tcaaggcgcc cctgtccttg ttgtgttgtc catgaaaacg ggcgcttatg
     5341 cgccgtggcc ggccgccttg cgcagcgcgc ggaccttgtc ggttttctcc caggtgaact
     5401 cgggaagctc gcgcccgaag tgaccgaggg acgccgtctt ccggtagatg gggcgcagca
     5461 ggttcaactc gcggatgatc cgggcgggcc gcaggtcgaa ggtgtccatc accgcgcgcc
     5521 cgatccgctc ctccgggacg tttcccgtgc cgaaggtttc aacgaggacg gacaccggct
     5581 cggcgacgcc gatggcgtag gcgagctgca cttcgcattt gcgcgcaagc cccgcggcaa
     5641 cgacgttctt ggccacgtac cgggccatgt acgccgcgct gcgatcgacc ttggaaggat
     5701 ccttccccga gaacgccccg ccgccgtgcc ggctgtaccc cccgtaggtg tcgacgatga
     5761 tcttccgtcc gg
//
LOCUS       DASVJL010000113         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_861525, whole genome shotgun sequence.
ACCESSION   DASVJL010000113 DASVJL010000000
VERSION     DASVJL010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861525"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VF357_02170"
     CDS             <1..237
                     /locus_tag="VF357_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522980.1"
                     /translation="IGFEMINPTLRGKRDFQHFFQVPVLASIPVIRDTEYEARNSRQR
                     TVVYSGLISFGALVILFLVVFGQKVHNLLHGLFS"
     gene            234..1010
                     /locus_tag="VF357_02175"
     CDS             234..1010
                     /locus_tag="VF357_02175"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX7522981.1"
                     /translation="MIKLPFFRKNGKPPATRRPVDLFAVGKEDILFHEKFKGLRAMVE
                     QKADLHQMRILGITSSVAGEGKTTICAHLGRSLASTGRKKVLLVDLDVRKANLTRGMG
                     AHRSPGLTEYLLGGDTIGQFIRWTHVPNLSLITSGIEVNSPADLLAGEKFKSFLQEIR
                     TMYDMILLDTPPVLPVADTPTIRDLTDGFLFVYRAGFTPYTMLRQAMEELGEKNVLGA
                     ILNGVEVASDSYYRKYYGSYYHRGVEAVIDSAKTSEAIRK"
     gene            1007..>1540
                     /locus_tag="VF357_02180"
     CDS             1007..>1540
                     /locus_tag="VF357_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522982.1"
                     /translation="MKAPFAFLDLLNPIRWFRRNAAFHRIHPTERFRDLIERERSRTD
                     RTGICFSLVVFRLKGRKRDTYQTLYHLTNMLLSQARTSDEIGWFDKTSLAILLIGAEE
                     PGAAMFGQKMTASLPEGVAVPTVSVYPYPNKGGGEGQAAPRKEDVREFGTSAAIPQTP
                     VAEETALPKNAVMPSVFA"
BASE COUNT          309 a          471 c          462 g          298 t
ORIGIN      
        1 atcgggttcg agatgatcaa tcccacgttg cgcgggaagc gggatttcca gcacttcttc
       61 caggtgccgg tgctcgcctc gatcccggtc atccgcgaca cggaatatga agcccgaaat
      121 agccgtcaac gaacggtcgt ctacagtgga ctgatctctt tcggcgcgtt ggtcatcctt
      181 ttcctcgtcg tcttcggtca aaaggtccac aacctccttc acgggctgtt ctcatgatca
      241 agctcccgtt tttccggaag aacggaaagc cgcccgctac tcgccgcccg gtcgacctct
      301 tcgcggtggg gaaagaggac attctcttcc acgagaagtt caaggggctc cgggccatgg
      361 tggaacagaa ggcggacctt caccagatgc ggattcttgg gatcaccagt tcggtcgccg
      421 gcgaggggaa gacgaccatc tgtgcccacc tgggtaggag cctcgcatcg accggccgaa
      481 agaaggtcct gttggtggac ttggacgtcc ggaaggcgaa tctgacccga gggatgggcg
      541 cacatcgctc tccaggcctg accgaatacc tgctcggagg agacacgatc gggcagttca
      601 tccgttggac ccatgttccg aatctgtccc tgatcacttc cggcatcgag gtgaattctc
      661 ctgcggacct gctggcgggg gagaagttca agtcctttct gcaagagatc cgtacaatgt
      721 acgacatgat cctcctcgac actccccccg tccttcccgt cgccgataca ccgacgatcc
      781 gagacctgac ggacggattc ctctttgtgt accgggccgg attcaccccc tacacgatgc
      841 tccgccaggc gatggaggaa ctgggggaga agaacgtgct cggcgccatc ctcaacgggg
      901 tggaggtggc gagcgattcg tattaccgga aatattacgg ctcctattac caccggggcg
      961 tggaggccgt gatcgattcg gcgaaaacca gcgaggcgat ccgaaagtga aggctccgtt
     1021 cgcctttctc gacctcctca acccgatccg ctggttccgg aggaacgcgg cgttccaccg
     1081 gatccacccc acggaacggt tccgtgatct catcgagcgg gagcgttccc gcaccgaccg
     1141 cacggggatc tgcttttcgt tggtggtgtt ccgcctgaag ggaaggaaac gggacacata
     1201 ccaaacgttg taccacctta cgaatatgtt gttgtcccag gcccgtacca gcgacgaaat
     1261 cggctggttc gacaaaacct ccctggcgat cctcctgatc ggggcggagg agcccggcgc
     1321 cgccatgttc gggcagaaga tgaccgcctc gctgcctgag ggagtcgccg tcccgacggt
     1381 ctcggtgtac ccctacccga acaagggggg aggtgaagga caggctgcac cgcgcaaaga
     1441 agacgtgagg gaattcggga caagtgcggc gatcccgcag acccccgtgg ccgaggagac
     1501 cgcccttccg aaaaatgcgg tgatgccgtc ggtcttcgcc
//
LOCUS       DASVJL010000114         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1049108, whole genome shotgun sequence.
ACCESSION   DASVJL010000114 DASVJL010000000
VERSION     DASVJL010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049108"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VF357_02185"
     CDS             <1..381
                     /locus_tag="VF357_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7522983.1"
                     /translation="TRIINGIGGSGDFARNSFISIFTTSSVAGRNGSVSCVVPMVSHV
                     DHCEHDVDVVVTEQGLADLRGTSPKQRAVQVIDKCVHPDYRPMLKDYYQRALYSSPGK
                     HTPHLIDEAFAWHLRFQRTGSMKV"
     gene            432..629
                     /locus_tag="VF357_02190"
     CDS             432..629
                     /locus_tag="VF357_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX7522984.1"
                     /translation="MKRVPRVNKGECISCGVCVDTVPAVFRFDAEDRAEVYDPNGADA
                     AAIQEAIDLCPVSCIYWDEEE"
     gene            626..1279
                     /locus_tag="VF357_02195"
     CDS             626..1279
                     /locus_tag="VF357_02195"
                     /inference="COORDINATES: protein motif:HMM:NF017459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNA1162 family protein"
                     /protein_id="HEX7522985.1"
                     /translation="MTLRSRQRRAAAVSALILVSVLLSASGCGGRKEVRPEPSPVVVP
                     TGPKVAVAPMENKSNDLSASDIIRDAFVEGVARKGYAVVPVAESDRILRETLGISYGG
                     QLPSTTPEEVCKALGVEAVFYGEVQEWSKTTTGIYNSSTVAAAFRMYREDGALQWEGN
                     DRQVRQDVARGGGNLGAGIIAGALGNLLLNPMTPQGRRVGWNIARKLPMGVLDNTSR"
     gene            1324..2286
                     /locus_tag="VF357_02200"
     CDS             1324..2286
                     /locus_tag="VF357_02200"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEX7522986.1"
                     /translation="MRKRLLLGGILFMGIAFAGCATAPPVTGPSTRDSAPAAEYGYRK
                     SEQAAPIVSKGPKKRVAVVKFQDKSAYGRGRLGSAIQDILTTELARSGQFIMVTRQDL
                     DLILDEQDLAKSGTIKAGTGAKSGEVLGVNAIVTGVVSQFGVKQKSATYLVGASKTQT
                     AEATVDVRVVDATTGRIIYAESGNGVYETSSTEVLGIGGRTGYDETIEGKAFRAAVSK
                     FIDNLIGKMAAMEWTGKVASVEGGEVTVNAGKKTGLVIGDRLRVYGEGREVIDPDTKV
                     SLGRRPGAEKGEIEIVDFFGEDAAIGKVLQGRGFAVNDIVRFGK"
     gene            2312..2635
                     /locus_tag="VF357_02205"
     CDS             2312..2635
                     /locus_tag="VF357_02205"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX7522987.1"
                     /translation="MRSVPVLSLVVTLLAAVVLLGGCYPKSQVYGVGNDGGLVFSVNP
                     PEAEVFLDGVGQGTSSMFTEDRYLKVSAGKHVLELRLAGYETYSREIFVSNSLLRIEA
                     GLIKR"
     gene            complement(2660..>3521)
                     /locus_tag="VF357_02210"
     CDS             complement(2660..>3521)
                     /locus_tag="VF357_02210"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7522988.1"
                     /translation="NALLSRSRVLVLSPLFSSDLETILRRALTDADRGLGKPETFLAP
                     EALRHIVAVADGDARVALNALEAAVAIAEHKGLSEVDMGTAEEALRKKALLYDKDGEE
                     HYNLISALHKSLRGSDPDAALYWLARMLSAGEDPLYVARRMIRFASEDIGNAAPGALQ
                     ITLAAAEAYRTLGSPEGELALAQAAVYLATAPKSNRVYTAWLKATRDAETEGTAPVPL
                     HLRNAPTRMMKELGYGKEYKYPHDFADAFVPENYFPETLGRRRYYEPSEAGHEKVIAD
                     RLKAWWSGRK"
BASE COUNT          603 a         1070 c         1282 g          566 t
ORIGIN      
        1 acgcggatca tcaacggcat cggcggctcc ggcgacttcg cgcggaactc cttcatctcc
       61 atcttcacca cctcctcggt ggcggggagg aacgggagcg tctcctgcgt cgtgccgatg
      121 gtgtcccacg tggaccattg cgaacacgac gtggacgtgg tcgtcacgga gcaggggctg
      181 gcggacctgc ggggcacatc gcccaaacag cgcgccgtcc aggtcatcga taaatgcgtc
      241 caccccgact accggccgat gctgaaggat tactaccagc gtgcgctgta cagttccccg
      301 ggaaagcaca cgccgcacct gatcgacgag gcgttcgcgt ggcacctccg gttccagcgg
      361 accgggtcga tgaaagtgtg agggggctat aatgacgtgg gaaacgacgg catgttgtcc
      421 cggagttgaa gatgaagcgg gttccgcgcg tcaataaggg agagtgcatc tcctgcgggg
      481 tgtgcgtcga tacggtgcct gcggtgttcc ggttcgacgc ggaggaccgc gccgaggttt
      541 acgacccgaa cggcgccgac gcggcggcga tccaggaggc gatcgacctt tgcccggtga
      601 gctgcattta ttgggacgag gaggagtgac gctccgttcc cggcaaagac gggcggcggc
      661 cgtatcggcc ctgatcctcg tttcggtcct cctgtccgca tcgggctgcg gggggaggaa
      721 ggaggtccgt ccggagcctt cgccggtggt cgtcccgacg gggccgaaag tggccgtggc
      781 gccgatggag aacaagagca acgatctctc ggcgtccgac atcatccgcg acgcgttcgt
      841 ggagggggtg gcccggaaag ggtatgcggt ggtgccggtg gccgagagcg accggatcct
      901 gcgcgagacg ctggggatca gctacggcgg gcagctcccc tccaccacac cggaggaggt
      961 atgcaaggca ctgggggtcg aggcggtctt ctacggcgaa gtgcaggagt ggagcaagac
     1021 gacgacggga atctataaca gttccaccgt cgcggccgcg ttccggatgt atcgggaaga
     1081 cggggcgctc cagtgggagg ggaacgatcg ccaggttcgc caggacgtcg cccgcggggg
     1141 gggcaacctg ggggccggga tcatcgccgg agcgttgggc aacctgttgc tgaacccgat
     1201 gacgccgcag ggaaggcgcg tcggttggaa catcgcaagg aagcttccga tgggcgtgct
     1261 ggacaacacg tcgcgctgag gagagacaaa ggcgcagtga cacttatcga aaggggtgga
     1321 ccgatgagga aacggcttct gttgggaggg atcctgttca tgggcatcgc gttcgcaggt
     1381 tgcgcaacgg cgccgccggt cacggggccg tccacgcggg actccgcgcc cgccgcggag
     1441 tacgggtacc ggaagtcgga gcaggcggcg ccgatcgtat cgaagggacc gaaaaagcgg
     1501 gtggccgtcg tcaagttcca ggacaagagc gcctacggcc gcggccgcct gggcagtgcg
     1561 atccaggaca tcctcaccac cgagctcgcg cgctccggcc agttcatcat ggtgacgcgg
     1621 caggacctcg acctgattct ggacgagcag gacctggcga agagcgggac gatcaaggcg
     1681 gggacggggg cgaagtccgg cgaggtgctg ggggtcaacg ccatcgtgac cggcgtcgtg
     1741 tcccagttcg gcgtgaagca gaagtcggcc acctatctcg tgggggcgag caaaacgcag
     1801 acggcggagg cgaccgtgga cgtgcgggtg gtcgacgcga cgaccgggcg gatcatctac
     1861 gcggagagcg ggaacggcgt ttacgagacc tcctccacgg aggtgctggg gatcggcggc
     1921 cgaaccggct acgacgagac gatcgagggg aaggcgtttc gtgcggccgt ctccaaattc
     1981 atcgacaacc tgatcgggaa gatggcggcg atggagtgga ccggcaaggt cgcgtcggtg
     2041 gagggcggag aggtcacggt gaacgcgggg aagaagaccg ggctcgtcat cggcgaccgc
     2101 ctccgggtgt atggcgaggg gcgtgaggtg atcgaccccg acacgaaggt gtccctgggc
     2161 cgccgccccg gcgccgagaa gggcgagatc gagatcgtcg acttcttcgg cgaggacgcc
     2221 gcgatcggca aagtgctcca ggggagaggg ttcgccgtca acgacatcgt ccggttcggg
     2281 aaatagggag gcggccatga ggagaacgat catgcgatcc gttccggtcc tgtcgctcgt
     2341 cgtgacgctg cttgccgccg tcgtcctgct cggggggtgc tacccgaaga gccaggtcta
     2401 cggcgtcggc aacgacggcg ggctcgtctt cagcgtgaac ccgccggagg ccgaggtgtt
     2461 cctcgacggc gtggggcagg ggacctcttc gatgttcacg gaagaccggt acctgaaggt
     2521 gtccgcgggg aagcacgtcc tggagctgcg gctcgccggc tacgagacgt actcgaggga
     2581 gatcttcgtg tcgaattcgc tcctccggat cgaggcgggg ctgatcaagc ggtaaaccgg
     2641 aagaggtgcg tggggcgggc tacttcctgc cgctccacca cgccttgagg cggtcggcga
     2701 tcaccttctc gtggcccgcc tcggacggct cgtaatatct gcggcggccg agcgtttcgg
     2761 ggaagtagtt ttccgggacg aaggcgtcgg cgaaatcgtg cgggtatttg tactccttcc
     2821 cgtatccgag ctccttcatc atccgggtgg gggcgttccg caggtgcagc ggcaccgggg
     2881 cggtcccttc cgtttccgcg tccctcgtcg ccttcagcca ggcggtgtag acccggttgc
     2941 tcttcggggc ggtcgcgagg tacacggccg cctgcgccag cgccagttcc ccctcggggc
     3001 tccccagcgt ccggtacgcc tccgccgccg ccagcgtgat ctgcagcgcc cccggcgcgg
     3061 cgttgccgat atcctccgag gcgaaccgga tcatcctgcg ggcgacgtag agcgggtcct
     3121 ctcccgcgga gagcatccgc gcgagccagt agagggcggc gtccgggtcc gacccccgca
     3181 ggctcttgtg gagcgcggag atcaggttgt aatgttcctc cccgtccttg tcgtacaaca
     3241 gcgccttttt ccgaagcgcc tcctccgccg ttcccatgtc gacttcggaa aggcccttgt
     3301 gctccgcgat ggcgaccgcc gcctccagcg cgttcagcgc cacccgggcg tcgccgtccg
     3361 ccaccgccac gatgtgccgc agcgcctccg gggcaaggaa cgtttccggc ttccccaacc
     3421 cccggtccgc gtcggtcagc gcccgccgga ggatcgtttc gaggtccgaa gaaaacaagg
     3481 gagaaaggac cagcacgcgc gagcgggaaa ggagcgcgtt c
//
LOCUS       DASVJL010000115         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1424757, whole genome shotgun sequence.
ACCESSION   DASVJL010000115 DASVJL010000000
VERSION     DASVJL010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424757"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VF357_02215"
     CDS             complement(<1..539)
                     /locus_tag="VF357_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEX7522989.1"
                     /translation="MAVKIGINGFGRIGRNFFRAAYKDPSLQIVAVNDITDAKTLAHL
                     LKYDSVHGRFDASVEVKENAIVVNGKEVQVLSCKDAADLPWGKLGVEIVIESTGRYTD
                     RDGAGKHIAAGAKRVIISAPSRGEDGTFVMGVNEKTFDPAKHFILSNASCTTNCLAPV
                     AKVLLDTFGIERGLMTTIHS"
     gene            679..>1574
                     /locus_tag="VF357_02220"
     CDS             679..>1574
                     /locus_tag="VF357_02220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX7522990.1"
                     /translation="MKRRTIVVTRRLPGVPWDDLAKRFRAGDPPKEGTMPREEFLARA
                     RGASGILCTLADRVDAELMDAAGPSLVVVSNFAVGVNNVDVAGATRRGIRVCNTPDVL
                     TDATADLGFALLLSAARKVSDADRFVRAGGWTGWDPWGFLGVPVAGKTLGIVGMGKIG
                     TAVARRSRGFSMKVLYNNRRRIPPSEEAELAASYRDLDALLAESDFVVLCIPLTPETR
                     GLLSTERLARMKRTAVLVNISRGDVVDEEAVAAALAEGRLFGAGLDVFEKEPRIHPRL
                     LSAPSAVLLPHLGSATGETREA"
BASE COUNT          237 a          490 c          580 g          267 t
ORIGIN      
        1 gagtggatcg tggtcatcag gccgcgctcg atcccgaagg tgtcgagcag caccttggcg
       61 accggggcga ggcagttcgt ggtgcaggag gcgttggaga ggatgaaatg cttcgccggg
      121 tcgaacgtct tctcgttgac ccccatgacg aaggtgccgt cctcccccct cgacggggcg
      181 gagatgatca cgcgcttcgc gcccgccgcg atgtgcttcc ccgcgccgtc ccggtccgtg
      241 tacctgccgg tggactcgat gacgatctcg acccccagct tgccccacgg cagatcggcg
      301 gcgtccttgc acgaaaggac ctggacctcc ttgccgttca ccacgatcgc gttttccttc
      361 acctcgacgg acgcatcgaa gcggccgtgc acggagtcgt acttcaagag gtgggccagc
      421 gtcttcgcgt cggtgatgtc gttgacggcc acgatctgga gcgagggatc cttgtacgcg
      481 gcgcggaaga agttgcggcc gatccgcccg aacccgttga tgccgatttt gacggccata
      541 tcgcgaacct cctgcacggt aataggatga tcctgccgga aagggaagat tataccctct
      601 gcgggccttc tgtcaattat ttcgggtata taatgaaacc ccccggggac ggaagatccc
      661 cgcgggaggg ggcggatcgt gaagcggcgg acgatcgtgg tgacgcggcg cctgccgggg
      721 gtgccgtggg acgacctggc gaagcggttc cgggcggggg acccaccgaa agaggggacg
      781 atgccccggg aggagttcct tgcccgcgcc cggggggcgt cggggatcct ctgcacgctg
      841 gcggaccggg tggacgcgga actgatggac gcggccgggc cctcccttgt cgtcgtgtcg
      901 aacttcgccg tcggggtgaa caatgtcgat gtggccgggg cgacccggcg cgggatccgg
      961 gtctgcaaca cgccggacgt tctgacggac gcgacggccg acctcgggtt cgccttgctc
     1021 ctttccgcgg ccagaaaggt gtcggacgcc gaccggttcg ttcgcgcggg gggctggacc
     1081 gggtgggatc cgtgggggtt cctcggagtc cccgtcgccg ggaagacgct ggggatcgtc
     1141 ggaatgggga agatcggcac cgcggtggcc cgccggtccc gcggcttctc gatgaaggtc
     1201 ctctacaaca accgacgccg gattcctccg tcggaggagg ccgaactcgc ggcgtcgtac
     1261 cgcgatctcg acgcgctgct cgcggagagc gacttcgtcg tgctgtgcat ccccctcacc
     1321 ccggagaccc ggggactgct ctccacggag cgcctcgcga ggatgaaacg gacggcggtc
     1381 ctggtgaaca tctcccgggg agatgtggtg gacgaggagg cggtggcggc ggcgctggcg
     1441 gaggggcggc tgttcggcgc ggggctcgac gtgttcgaga aggagccgcg gatccacccc
     1501 cggctgctgt cggccccttc ggcggtgctc ctgccgcacc tcgggagtgc gaccggggag
     1561 acgcgggagg cgat
//
LOCUS       DASVJL010000116         6657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_298708, whole genome shotgun sequence.
ACCESSION   DASVJL010000116 DASVJL010000000
VERSION     DASVJL010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298708"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /gene="dnaJ"
                     /locus_tag="VF357_02225"
     CDS             <1..793
                     /gene="dnaJ"
                     /locus_tag="VF357_02225"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX7522991.1"
                     /translation="GGGGRSRRGADLRYNLTVTFEEAVFGSEKEIVVPRTGVCRDCSG
                     TGARKGTRPERCGACNGQGHVTVQQGFFAIRRTCGRCGGTGQVVKDPCGSCAGTGHVR
                     ESRTLKVKIPPGVDSGTRLKLRGEGEAGPAGGIGGDLYVVLTVKEHAFFVREGADLFC
                     EVPITFPQAALGATIEVPTLSGKKNLSIPPGTPSGHDFMMRGEGVAALNSGRRGNLVI
                     RVLIEVPRKLTKRQKEILAEYQELSEESPGPISRSFFEKVKEIIG"
     gene            790..2739
                     /locus_tag="VF357_02230"
     CDS             790..2739
                     /locus_tag="VF357_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7522992.1"
                     /translation="MTCCRGILTACLFVFLLPVFSVSAGAAEALRPLGGGADAIPVPR
                     PGARESGRTAPIVPFLQAESEYAAGKTDEALSRFLDLAYSAADDERKGFIWWRVGELL
                     LVRGDLDKALEAADKAVLLSRAPYLSLSAADLKLRIYQRMKWNNEARQMAAYLLDRKF
                     VGVDLPALLALMARMDAGAGKLGSALAFYRRAVAATMDPQASRQLSAERESLIEGATD
                     LSVLRGAAEGEEDPEVRAHLYLAMGNAAIRKGFLGMAAYTFERSARAGGKWSREAAER
                     LFRAEKIIAARPRIVGLVPLSGKLSDIGYAVLTGAEVALGTLPGFERNGATPIIRWVD
                     TAGEPEKARQEFVDAAADRMVLGILGPVTGEEGRSVGAAFTQKSPPTLYLGQKPIFGK
                     PFLYGFGLSPAQEARALLAHLARVGVSDLLLLHPENGYGDGFAAAVAAAAPEAGIRIA
                     KTVSYSPETQDFTEVIKRAVGNATFRRQSRSKEKGKAMKLPLGGIIIADRWDRVFLLA
                     SQLRYYNVYLPLAGFSGWNDPELLRKAVGSVSGAVFPVDYSEVIPGSQGDRFRNAFQE
                     MMRVPPTRFEAMGYDGALFLSEAFALDGGSGRPLGEAMRERIPRLKNYPGVTGSFQFT
                     PAGDLRRKVPLLQVELGNFVPVSTP"
     gene            2775..4757
                     /locus_tag="VF357_02235"
     CDS             2775..4757
                     /locus_tag="VF357_02235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX7522993.1"
                     /translation="MSLSPGGPIAAAMPGFESRPGQLRMALAWAEALETPRVLVAEAA
                     TGIGKTLAYLVPAILSGRKTIISTGTRTLQQQLMENDIPLVRDILQYPFSCTVLKGRG
                     NYLCLRRWKRFRAEPLFEFAREAGYFSAMQAFAETTRTGDVSECIGVPEDARVWGEVN
                     ARSEMCDASTCGETERCHLAAARRRAADADLVVVNHHLFFADLALRERLGPGRGGEEG
                     RFGEVLPRADAVVFDEAHGVEETASVFFGVTVSLGRARELARDVKRSAAKAGGGWDAL
                     LPMAERFRLVAESAFDAVGDGEARFALPAPSADTPFGQKASALLRAGEELALSLSDGA
                     PRGERGPDPGTDRDPLLRRVRAFLDDLGSVLSSDSASAVAWAERRGASVSLHRTPVEV
                     SPVLSGALWGERIPFLLTSATLSVSGDLSYFRERVGLARVDARELIVDNEFDFAGRAL
                     FYVPQGLPDPSDSAFPAAAAEETRKILSCSGGGALVLCTSYRTLSALTEALRGTLPHT
                     LYVQGDAPRGHLLRAFREGEDTVLIGTGTFWEGVDVPGASLRCVVIDKLPFASPSDPV
                     TSARIRLLRDRGADPFMEYQLPEAVLSLRQGVGRLLRRGDDYGVVALLDRRVSTRGYG
                     ELFRSNLPPARWTDDRDEVAAFFRRFHGEKKKEEKG"
     gene            4754..5620
                     /gene="galU"
                     /locus_tag="VF357_02240"
     CDS             4754..5620
                     /gene="galU"
                     /locus_tag="VF357_02240"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX7522994.1"
                     /translation="MIRKAVFPAAGFGTRFLPATKASPKEMLPLVDMPLIQHGVEEAK
                     AAGVRDMIIVTGRGKNAIEDHFDVSFELEAMLMKKGDSALLSSVRRVTDGADFFYVRQ
                     NLPLGLGHAVLRSMDLVGQEPFAVVLSDDVIDAKVPVLKQMIEAYEKYRAGAVLAIQR
                     VPRNAVSRYGIIRGKRIANGVYDVKDMVEKPKPADAPSDLAIIGRYLLPPSIFPALLA
                     TKPGAGGEIQLTDAIRSLIGTERIIGLEFKGVRYDAGTKIGFQMANIAFALKDPEIGP
                     GLRAFLKKEKLT"
     gene            5617..6072
                     /locus_tag="VF357_02245"
     CDS             5617..6072
                     /locus_tag="VF357_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7522995.1"
                     /translation="MSRKIGVSRTRQKSPGEVRAEGSAARRTGHVLLLDLPVDSACQP
                     PSDVVEVDDAVRVLLEIPGVPPASVQVRVLGNRIEVTGEKIPDFPAGETSFLCLERIF
                     GKFQRAFEVRGSVNLGEVSARMANGILVITIPKVAERRGQERRIPVTTG"
     gene            6101..>6657
                     /locus_tag="VF357_02250"
     CDS             6101..>6657
                     /locus_tag="VF357_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX7522996.1"
                     /translation="MTDDAKKTNPPEGTDAPAERTDEGSPPAASVAEPEAETPKGETP
                     SPEKEGGPEIPEVLPLLPVRDIVIFPYMTLPLFVGREGSVAAVDEALAQDRYIFLATQ
                     RDPSVEDPKEVDLYRTGTVAMIMRMLKLPDGRLKILIQGVVKAKIVEFIEAKPAVRVR
                     IDRIVESPMKEGSLEVEALMRASRE"
BASE COUNT         1030 a         2136 c         2414 g         1077 t
ORIGIN      
        1 cggaggaggc ggtcgctccc gccgcggcgc ggatctccgg tacaacctga cggtcacctt
       61 cgaggaggcg gtcttcggct ccgagaagga gatcgtcgtt cccaggacgg gggtgtgccg
      121 cgactgctcg ggaacgggcg cgcggaaggg gacacgaccg gaacgatgcg gggcctgcaa
      181 cggacaaggc cacgtcacgg tgcagcaggg gttcttcgcg atccgccgca cttgcggccg
      241 atgcgggggg acgggccagg tcgtcaagga cccgtgtgga agttgtgccg gcaccggcca
      301 cgtccgggaa agccgcacgc tcaaggtgaa gatccccccc ggggtcgaca gcggcacgcg
      361 cctcaagttg cggggcgagg gggaggccgg gcccgcggga ggaatcggcg gcgacctgta
      421 cgtggtgctt accgtgaagg agcacgcgtt ctttgtccgg gaaggggcag accttttctg
      481 cgaggtgccg atcacctttc cgcaggcggc gttgggggcg acgatcgagg tacccaccct
      541 ttccggaaag aaaaacctct ccatcccgcc cggcacgccg tccggccatg atttcatgat
      601 gcggggggag ggggtcgcag cgctcaactc cggccggcgg ggaaacctcg tgattcgcgt
      661 gctgatcgag gtgcccagga aactaacgaa gcgccagaag gagatcctcg cggagtacca
      721 ggaactgtcc gaggagtccc cgggtcccat ctcccgaagt ttcttcgaga aggtgaagga
      781 gatcatcggg tgacctgctg ccgcgggatc ctaaccgcct gcctgttcgt cttcctgctc
      841 cctgtttttt cggtttccgc cggggccgcc gaggctctcc ggccgctggg tggcggggcc
      901 gacgcgatcc ccgttccccg ccctggggcc cgcgagtccg ggcggacggc gccgatcgtc
      961 cccttccttc aggccgagag cgaatacgcc gcggggaaga ccgacgaggc cctctcccgg
     1021 ttcctcgacc tcgcatactc cgccgcggac gacgagcgga aagggttcat ctggtggcgc
     1081 gtcggggaac tgctgctcgt ccgcggggat ctcgacaagg cgctggaggc ggcggacaag
     1141 gcggtgctcc tctcccgcgc tccgtatctc tccctctcgg ccgccgatct caagctgcgg
     1201 atctaccagc ggatgaagtg gaacaacgag gcgcggcaga tggcggcgta cctcctcgac
     1261 cggaagttcg tgggcgtgga ccttcccgcg ctcctggccc tgatggcccg tatggacgcc
     1321 ggggcgggga agctgggcag cgcgcttgcg ttctaccgcc gcgccgtcgc cgccaccatg
     1381 gatccgcagg catcccggca gctgtcggcc gagcgggaat cgctcatcga gggagcgacg
     1441 gacctctccg tgctccgggg ggccgccgag ggggaggagg atccggaggt tcgcgcccac
     1501 ctgtatctcg cgatggggaa tgcggcgatc cggaaaggat tcctcgggat ggcggcctac
     1561 acgttcgagc gatcggcgcg ggccggaggg aaatggtccc gggaggccgc ggagcgcctg
     1621 ttccgggcgg agaagatcat cgccgcgcgg cccaggatcg tcgggctcgt ccctctctcg
     1681 ggaaaacttt ccgacatcgg gtacgcggtc cttaccgggg cggaagtggc cctcggcacc
     1741 ctccccgggt tcgagcggaa cggcgcgacg ccgatcatcc ggtgggtgga cacggcgggg
     1801 gagcccgaga aggcgcggca ggagttcgtt gacgcagcgg ccgaccggat ggtgctcggc
     1861 atcctcggtc cggtcaccgg agaggagggc cgatcggtcg gcgccgcgtt cacgcagaaa
     1921 tcgccgccca cgctgtatct cggccagaag ccgatcttcg gaaagccgtt cctctacggg
     1981 ttcggactct ctccggcgca ggaggcgcga gcccttctcg cgcacttggc gcgggtaggg
     2041 gtttccgacc tgctcctgct ccacccggag aacggctacg gggatggctt cgccgctgct
     2101 gtggccgcgg ccgccccgga ggcggggatc cggatcgcga agaccgtgtc gtactccccg
     2161 gagacgcagg acttcacaga ggtgatcaag agggcggtcg gcaacgcgac tttccggcgc
     2221 cagtcgcgat cgaaagagaa ggggaaggcg atgaagcttc cgctgggggg gatcatcatc
     2281 gccgaccggt gggaccgggt cttcctcctt gcgtcgcagt tgcgctacta caacgtctat
     2341 ctcccgctgg ccgggttctc cgggtggaac gaccctgaac tgctgaggaa ggcggtcggt
     2401 tccgtttccg gggcagtctt cccggtggac tactccgaag tgatcccggg atcccagggc
     2461 gaccggttcc ggaatgcgtt tcaggagatg atgcgtgttc cccccacccg gttcgaggcg
     2521 atggggtacg acggcgccct gtttctctcc gaggcgtttg cgctcgacgg cggttccgga
     2581 cgtcccctcg gggaagcgat gcgcgagagg attccccggc tgaaaaatta ccccggcgtg
     2641 acgggatcgt tccagttcac cccggcaggg gatctgcgtc ggaaagtccc cctcctgcag
     2701 gtcgagctgg ggaacttcgt ccccgtctcg accccgtaaa gcgcgtgccc ggagatctcg
     2761 cccggaaagt ggggatgtcg ctctcccccg gcggtccgat cgcggcggcg atgcccgggt
     2821 tcgagtcgcg cccggggcag ctccggatgg cgctcgcatg ggcggaggcg cttgaaacgc
     2881 cccgcgttct ggtcgcagag gcggccaccg ggatcggcaa gacgctggcc tacctcgtcc
     2941 ccgcgatcct ctccggccgg aagacgatca tctccaccgg tacgcgcacc ctccagcagc
     3001 aactgatgga aaacgacatt cccctggtgc gggacatcct tcagtacccc ttttcgtgta
     3061 ccgtcctcaa aggccgcggc aactacctct gcctccgccg ctggaagcgg ttccgtgccg
     3121 agccgctctt cgagttcgcc cgggaggccg gctacttctc cgcgatgcag gcgttcgcgg
     3181 agacgacccg gacgggggac gtctccgagt gcataggggt gcccgaggac gcgcgcgtct
     3241 ggggagaggt gaacgcccgc agcgagatgt gcgacgcgtc gacgtgcggc gagaccgagc
     3301 ggtgccatct cgcggcggcc cggcggcggg cggcggacgc cgatctcgtc gtggtgaacc
     3361 accacctgtt cttcgcggac ctcgcgctgc gcgagcggct cggccccggt cgcgggggcg
     3421 aggaggggcg gttcggggag gtgctccccc gcgccgacgc cgtggtgttc gacgaggcgc
     3481 acggggtcga ggagaccgcc tccgtcttct tcggcgtgac ggtctctctc ggtcgtgcac
     3541 gggagctcgc gcgggacgtg aagcgttccg ccgcgaaggc gggcggcggg tgggacgccc
     3601 tcctgccgat ggccgagcgg ttccgtctcg tcgccgaatc ggccttcgac gcggtggggg
     3661 acggggaagc ccgtttcgca ctgcccgcgc cttccgccga taccccgttc gggcagaagg
     3721 cgtcggcact gctccgcgcg ggggaggaac tcgccctgtc gctctcggac ggcgcccccc
     3781 gcggggagag gggtccggat ccggggacgg atcgggaccc cctgcttcgg cgggtccgcg
     3841 ccttcctcga cgatctcggc tcggtcctct cctccgactc cgcctccgcc gtggcgtggg
     3901 cggagcggcg cggcgcgtcc gtctccctgc accggacgcc ggtcgaagtc tccccggtcc
     3961 tttccggagc actctggggg gagcggattc cattcctcct cacctcggcg accctctccg
     4021 tatcgggcga cctgtcgtat ttccgcgagc gggtggggct cgcacgggtt gatgcccggg
     4081 aactcatcgt ggataatgag ttcgacttcg cggggcgggc gctcttctat gtcccgcagg
     4141 gacttcccga cccgtcagac tccgcgtttc cggcggcggc ggcggaagag acgcggaaga
     4201 tcctgtcgtg ctccggcggc ggcgcgctgg tcctgtgcac gagctaccgg acgctctcgg
     4261 cgctgacgga ggcgcttcgg gggacgctgc cgcacacgct gtatgttcag ggggatgcgc
     4321 cgcgggggca tctcctgcgg gcgttccggg agggcgaaga cacggtgctg atcgggacgg
     4381 ggacgttctg ggagggggtc gacgttcccg gcgcctccct gcggtgtgtc gttatcgaca
     4441 agctgccgtt cgcctctccg tccgacccag tgacctcggc ccgcatccgg ctcctgcgcg
     4501 accggggagc ggacccgttt atggagtacc agctccccga ggcggtcctt tcgctccggc
     4561 agggcgtcgg gaggcttctt cgccgcgggg acgattacgg ggtggtggcg ctgctcgacc
     4621 gcagggtttc gacccggggg tacggcgagc tcttccggag caacctcccc ccggcgcggt
     4681 ggacggatga ccgggacgaa gtggcggcgt tcttccgccg gttccacgga gagaagaaaa
     4741 aggaggaaaa gggatgatac ggaaagcggt attccccgcc gcggggttcg gcacgcggtt
     4801 cctgccggcg acgaaggcct ctcccaagga gatgcttccg ctggtggaca tgcccctcat
     4861 ccagcacggg gtcgaggagg cgaaggccgc cggcgtacgg gacatgatca tcgtgaccgg
     4921 acgcggaaag aacgccatcg aggaccactt cgacgtctcc ttcgagctgg aggccatgct
     4981 gatgaaaaag ggggacagcg ccctcctctc ctccgtccgc cgcgtcaccg acggggccga
     5041 cttcttctac gtccgccaga acctcccgct ggggctgggt cacgccgtgc tccggtcgat
     5101 ggacctggtc ggccaggagc cgttcgccgt cgtcctctcg gacgacgtga tcgacgccaa
     5161 ggtccccgtg ttgaagcaga tgatcgaggc gtacgagaag tatcgcgccg gcgcggtcct
     5221 cgcgatccag cgcgtcccga ggaacgcggt ctcccggtac gggatcatcc gggggaagcg
     5281 gatcgcgaat ggggtgtacg atgtgaagga catggtggag aagccgaaac ccgcggacgc
     5341 gccgtcggac ctggcgatca tcgggcgata tctcctcccc ccgtcgatct ttccggctct
     5401 tctggcgacg aagcccgggg cggggggcga gatccagctg accgacgcca tccggtccct
     5461 gatcgggacg gagcggatca tcgggctcga attcaagggg gtccggtatg acgcggggac
     5521 gaagatcggc ttccagatgg cgaacatagc cttcgcgctg aaagacccgg aaatcggccc
     5581 ggggctgcgc gcatttctga aaaaggagaa attgacgtga gcagaaagat cggggtatcc
     5641 cgcacgcgcc agaagtctcc gggggaggtc cgggcggaag ggtcggcggc ccgccggacc
     5701 gggcatgtcc tgctgctcga cctcccggtg gactccgcct gccagccgcc ctcggacgtc
     5761 gtggaagtgg acgacgccgt ccgggtcctg ctggagatcc cgggagtccc gccggcgtcc
     5821 gtccaagtcc gggttctggg gaaccggatc gaggtcaccg gggagaagat cccggatttt
     5881 cccgcggggg aaacctcctt tctctgcctg gaacggatct tcgggaagtt ccagcgggcc
     5941 ttcgaagtga ggggttcggt gaacctcggg gaggtatcgg cgcggatggc gaacgggatc
     6001 ctcgtgatca cgattccgaa ggtcgcggaa cggcggggtc aggagcgccg gatcccggtg
     6061 actaccggat gaggaaaagg acgaaggggg gaacgacgac atgaccgacg atgcgaaaaa
     6121 gacgaatccg ccggagggga cggacgctcc cgcggaacgg accgacgagg ggagtccccc
     6181 ggccgcttcg gtcgcggagc cggaggcgga aaccccgaag ggtgagaccc cttccccgga
     6241 gaaggagggc gggccggaga tccccgaggt gctccctctg ctgccggtgc gcgacatcgt
     6301 catcttcccg tacatgacct tgccgctgtt cgtggggagg gagggttccg tcgctgcggt
     6361 ggacgaggcg ctggcgcagg accggtacat cttcctcgca acgcagaggg acccgtccgt
     6421 ggaagacccg aaggaggtgg acctctatcg cacgggcacg gtggcgatga tcatgcggat
     6481 gctgaagctc cccgacgggc ggctgaagat cctcatccag ggcgtcgtga aggcgaagat
     6541 cgtcgagttc atcgaagcga agcccgcggt gcgcgtccgg atcgaccgga tcgtcgagtc
     6601 cccgatgaaa gaggggtccc tcgaggtgga ggctctgatg cgcgcttccc gggagaa
//
LOCUS       DASVJL010000117         4421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1800466, whole genome shotgun sequence.
ACCESSION   DASVJL010000117 DASVJL010000000
VERSION     DASVJL010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800466"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(381..480)
                     /gene="ffs"
                     /locus_tag="VF357_02255"
     ncRNA           complement(381..480)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF357_02255"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            634..1425
                     /gene="otsB"
                     /locus_tag="VF357_02260"
     CDS             634..1425
                     /gene="otsB"
                     /locus_tag="VF357_02260"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX7522997.1"
                     /translation="MEGKPAGTIGAGIRSLWVFDFDGTLSPIVDDRQAARIHPMCREL
                     LKGLLRMPMQFVVVLSSREIEDLARRVPLPRVILGGASGLEWRLPGGHRIHPGDTFVA
                     RRKKVRGILDPLLSRLSFIPGVDVEDKGWSVAIHHRHVLPEVVAMLEPLLKEIEGTPD
                     VRVYRGPSVVEVQLLCNVNKSFGVRTICRIIGFDPSKDRIRYAGDDENDAVAIRWVLH
                     KGGIAFSVGDAVRVPGARVVENPAALARAVGALAEIPPQPGKGKG"
     gene            complement(1486..2718)
                     /locus_tag="VF357_02265"
     CDS             complement(1486..2718)
                     /locus_tag="VF357_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HEX7522998.1"
                     /translation="MGNENLKILHTDTLSPPRHAFRESVRLLKRNLTPQGIAAASTTP
                     ESAARNYTRVFCRDASISAMGMAVSGDPLLREGAMGGLEFLARHQAENGQIPNFVAPE
                     TGETDFWYLGCIDATLWWLAAVAFWSRHFPEDCVDEKFRGPIDAALRWLRCQEHQKIR
                     LLQQNEASDWADIMPRSGFVLYTNALWYHVKRAYSIAGAEETRADFNALFSPFAAELP
                     EYRRMRRLTRYVREGGKRGDLYLSYVNFSTWGGEGDVFGNLLAVLFGLADEGRANRIL
                     DDLLAAGVDDPNPVRAVCDPIRPSSPQWRIYMGRHRQNTEYQYHNGGAWPFLGGFWVV
                     ALASLGRSPEAADALSRVGRMNSVNGWAFHEWFHGRTGEPRGMRGQSWNAAAYILAER
                     SLERTALGIFLPERLPSL"
     gene            2886..>4421
                     /locus_tag="VF357_02270"
     CDS             2886..>4421
                     /locus_tag="VF357_02270"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX7522999.1"
                     /translation="MTRSRQRVVIENVSPEIDGGRFPVKRTAGQTVRVEADIHTDGQD
                     LIAAFLMYREAGSLIYSETPMIPFGNDRWAGEFRVAAVGRTVYTLQAWVARFLSWQRN
                     LSKKMEAGQEISVDLLVGTALAEDAAKRAKGADRRKLLAFARSFRPEGEARGVPECAA
                     AALDEELGALADRYPDRTLATRYGRELPVVADPERARFSTWYELFPRSCSMEPGRHGT
                     FKDVEKRLPYVASMGFDVLYLPPIHPIGRTNRKGKNNATVARPEDVGSPWAIGAAEGG
                     HKAVHPQLGTVEEFEELVRSARNHGIEIALDIAFQCAPDHPYVTEHPEWFRMRPDGTV
                     QYAENPPKKYEDIIPFDFDTPRWLELWEELKSVVLFWAGRGVRIFRVDNPHTKPFAFW
                     EWLIAEIKREHPDAIFLAEAFTRPKVMYRLAKAGFTQSYTYFTWRNTKPEIEAYFQGL
                     TQTEVREYFRPALWPNTPDILTEQLQYGGRPAFMARLVLAATLGASYGIYGPAFELCE
                     HEAT"
BASE COUNT          779 a         1459 c         1459 g          724 t
ORIGIN      
        1 tgccggagaa caggtaggcg tggtggatct tccccgaggt gatggcgttg gcgagggcgc
       61 gggtgacgtg cccctgcccg acgatctcgt cgaacgtctt cgggcgccac ttcctcgcga
      121 gcgcctcgta ggccatcggt tccgcgtccc tcccaggatg gcgtcgaatc aagcggtgcg
      181 tgtcgatact acggcaacga attgaacgag cgctcccgcg cggcgcgtcc tcgacgcctc
      241 ccccggctgc gccgcctcgg agcgggggct ccgttcgtgg ctcgccgtgc ggtgaacccg
      301 cacggctgcg ctttacctca ctgcgccccc ctcctgcggc ggctccgccg ggtccgcgct
      361 ttcgcgcccg cgggtccgcc ggcggccagg tgtgccgccg gcacacggag atgtgcggct
      421 gccgctgctt ccttccggac ctgacggggt tcgccgactt ccgttgcggc gggcctggcc
      481 gccggcgcac ccgcgcggtg ccaccggccg gtcgattgcg tatcgcaaag aggaatatcg
      541 tacgggaacc gggcccgcat ttcaaggacg gaccattaat acaaaacatt tgtgttaaaa
      601 ataagcaatc atcccaagga ggagggtttc aggatggagg ggaagcccgc tggaacgatc
      661 ggggccggca tccggtccct ctgggtcttc gactttgacg gaaccctctc ccccatcgtg
      721 gacgaccggc aggcggcccg gatccacccg atgtgccggg agctcctgaa aggactcctc
      781 cggatgccca tgcagttcgt cgtcgtcctc tccagccggg agatcgaaga cctcgcgagg
      841 cgcgtcccgt tgccgcgcgt catcctggga ggcgccagcg ggctcgagtg gcgtcttccc
      901 ggagggcatc ggatccatcc cggggacacg ttcgtcgccc gccggaagaa ggtccgcgga
      961 atcctcgacc ccctcctctc caggctttcc ttcatcccgg gggtggatgt tgaggacaag
     1021 ggttggtcgg tcgcgatcca ccaccggcac gtcctgccgg aggtcgtggc catgctggag
     1081 ccgctcctca aggaaatcga gggaacaccg gacgtccggg tgtaccgggg gccctccgtg
     1141 gtcgaggtgc agttgctctg caacgtgaac aaatccttcg gcgtccggac gatctgccgg
     1201 atcatcgggt tcgatccctc gaaagaccgg atacggtacg ccggcgacga cgagaacgac
     1261 gccgtcgcga tacggtgggt cctccacaaa ggggggatcg ccttctccgt cggcgacgcc
     1321 gtgcgcgtcc ccggggcgag ggtagtggag aatcccgccg ctctcgcacg ggcggtcgga
     1381 gctctcgcgg agatcccgcc acaaccgggc aaggggaaag gctgatcggc gttcgggctg
     1441 aagatgggag aaccagcgcg ttgcagacgt cggcgcgcag gccgcctaca gggacggaag
     1501 gcgctcgggc aggaaaattc ccaaggcggt ccgttcgagg gaacgttccg caagaatgta
     1561 cgccgccgcg ttccacgatt gcccccgcat cccgcgcggt tcccccgtcc tgccgtggaa
     1621 ccactcgtgg aacgcccacc cgttgacgct gttcatccgg cccacccggg aaagcgcgtc
     1681 ggcggcctcc ggactcctgc ccagggacgc gagcgccacc acccagaacc ccccgaggaa
     1741 aggccacgcg cccccgttgt ggtactggta ttccgtgttc tgccggtgcc tccccatata
     1801 gatgcgccac tgcgggctgg acgggcggat gggatcgcag acggcccgca cggggttcgg
     1861 gtcatcgacc cccgccgcca ggagatcgtc gaggatccgg ttcgcccttc cttcatccgc
     1921 caggccgaag agcaccgcga gcaggttccc gaagacgtcc ccctctccgc cccacgtgga
     1981 aaagttcacg tagctcaggt acaggtcgcc gcgtttcccg ccttcgcgga cgtaacgcgt
     2041 caagcggcgc atccggcggt attccggcag ttccgcggca aacggcgaga acagcgcatt
     2101 gaagtcggcc cgcgtctcct cggcgccggc gatcgagtac gcgcgtttca cgtggtacca
     2161 caaggcgttg gtgtaaagaa cgaaccccga tcggggcatg atgtcggccc agtcgctggc
     2221 ttcgttctgc tgcagcaggc ggatcttctg gtgctcctgg cagcgaagcc accggagtgc
     2281 ggcgtcgatc ggcccgcgga acttctcatc gacgcaatcc tccggaaagt gccgcgacca
     2341 gaatgcgacg gccgcaagcc accagagggt cgcgtcgatg caccccaggt accagaagtc
     2401 ggtctcgccg gtttccgggg cgacgaaatt cgggatctgc ccgttctccg cctggtggcg
     2461 cgcgagaaat tccagcccgc ccatcgcgcc ttcccggagg agcggatctc cggaaaccgc
     2521 cattcccatc gcggagatgg acgcgtccct gcagaagacg cgcgtgtagt tccgggcggc
     2581 cgattccggc gtggtggagg cggcggcgat cccttgcggg gtgaggttcc gtttcagaag
     2641 acggaccgac tcgcggaatg cgtggcgggg aggggaaagg gtgtcggtgt ggaggatctt
     2701 caggttttcg ttacccactc tcggatttgg tgcgtaacaa acggaaatag ttaagcatac
     2761 ccggccagac aatgccagcg ccaactcccg gaaccggcgc cgggccgggt tgctttgtgt
     2821 cacaggaacc agtataatcg tttgcataca aatcattccg ccccgaacac aatgggagga
     2881 cctctgtgac ccgatcccgg cagcgcgtcg tcatcgaaaa cgtatccccg gaaatcgatg
     2941 ggggtcgttt ccccgtgaaa cgaacggcag gacagaccgt gcgggtcgaa gcggatatcc
     3001 acacggacgg gcaagacctc atcgccgcgt tcctgatgta ccgtgaggcg gggagcctga
     3061 tctattcgga aactccgatg attccttttg gaaacgatcg ctgggccggc gaattccgcg
     3121 tcgccgccgt gggacggacc gtctacaccc ttcaagcctg ggtcgcccga ttcctctcct
     3181 ggcagcggaa cctctccaaa aaaatggaag caggacagga gatctccgtt gacctcctgg
     3241 tgggaaccgc cctcgcggaa gatgccgcga aacgggccaa gggcgcggac cgcaggaaac
     3301 ttctcgcctt cgcgaggtcc ttccgcccgg agggggaagc ccgcggcgtc ccggagtgcg
     3361 cggcagccgc gctggacgag gaacttggcg ccctggcgga ccggtacccg gaccgcaccc
     3421 ttgccaccag gtacggaagg gagttgccgg tggtggcgga tccggaaagg gcccggttca
     3481 gcacgtggta cgaactgttc ccgcggtcgt gctccatgga gccggggcgc cacggcacct
     3541 tcaaggacgt ggaaaagcgt ctcccgtacg tcgcctcgat ggggttcgac gtcctctacc
     3601 tccctccgat ccacccgatc gggcgcacca accgcaaggg gaagaacaac gcaaccgtcg
     3661 cccggccgga ggacgtcgga agcccatggg cgatcggcgc ggccgaaggg gggcacaagg
     3721 ccgtccatcc gcagctcggg acggtcgagg aattcgagga gctggtccgg tccgcccgga
     3781 accacgggat cgagatcgcg ctggacatcg cattccagtg cgctccggac catccgtacg
     3841 tcacggagca ccccgaatgg ttccggatgc gccccgacgg cacggtgcag tacgccgaga
     3901 acccaccgaa aaaatacgag gacatcatcc cattcgattt cgacaccccc cgctggctgg
     3961 aactctggga ggagctgaaa agcgtcgtgc tgttctgggc aggacgcgga gtccggatct
     4021 tccgggtcga caacccgcac accaagccgt tcgcgttctg ggaatggttg atcgccgaga
     4081 tcaagcggga gcaccccgac gcgatcttcc tcgccgaggc gttcacccgc ccgaaggtca
     4141 tgtaccggct ggcgaaggcg ggcttcaccc agtcctacac ctatttcacc tggaggaaca
     4201 cgaagccgga gatcgaggcg tatttccagg gcctgacgca gacggaggtc cgggagtatt
     4261 tccggcccgc cctgtggccg aacacccccg acatcctgac ggagcagttg cagtacgggg
     4321 ggcgtcccgc gttcatggcg cggctggtcc tggccgccac gctcggggcg agctacggga
     4381 tctacgggcc cgccttcgag ctgtgcgaac acgaggccac g
//
LOCUS       DASVJL010000118         9177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1425201, whole genome shotgun sequence.
ACCESSION   DASVJL010000118 DASVJL010000000
VERSION     DASVJL010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425201"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VF357_02275"
     CDS             complement(<1..560)
                     /locus_tag="VF357_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7523000.1"
                     /translation="MKRLALLLLSVVLVPNAFASDPALAKKEGKANFYANITAVEPIM
                     DAFTAAKGVKGVYTRISTTKYLATVLTEFEAGKLEADLLQGPLPILQALKEKGVLAAY
                     RSPAAAGYPGWARQDDTIVQFGIEYVALIYNKNLVKPGDVPTAYKDLANPKWRDKIVM
                     PDPSSHATTISWLVGLKEAGIFANDK"
     gene            803..3436
                     /locus_tag="VF357_02280"
     CDS             803..3436
                     /locus_tag="VF357_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7523001.1"
                     /translation="MTRILFPEGGDTKQALRQQRYLMAAGTAVVEIFLLALCWHQGIF
                     ALIHFLRAVAALTFINVLFFIMFRTGWNRRFTDPSLTLPQIIASFLVTTYLVYFAREA
                     RAIFLLFYLVTFLFGVFQLRPRQLYGIAFVAIASHVSVITLLWRFRPETMDLGIELAQ
                     WIVMAAVIFWFASMGVYTSNLRNKLRSSKVELENALRSVRDSAVDLQSAKERAEKANR
                     TKSEFLANMSHEIRTPMNGILGMTEILLHGDLPSEYRRDVELVHNSGEILLGIINNIL
                     DYSRIEAGKMELDDIPFDLAGVVDEIMKLLSMRAQSKEVELVSQVSPEIPSALRGDPG
                     RLRQILVNLVGNAVKFTERGEVVVRASLDSRDDGAVTVRFSVRDTGIGILPEAHAKIF
                     DSFTQADGSTTRKFGGTGLGLTISRQLVEMMGGTIRVESAPGKGSDFIFTIPLHTMEE
                     ADLRPSIPRPSLKGLKVLVVDDNRTNREILEKQLHDWASQSRGAGSGDEALSLLRVAA
                     AEGVSFDLAILDYHMPDIDGFQLAGLIKSDPSLSGVRLLMLSSVGIRGDGRKARETGI
                     SGYLSKPVRREDLYESIAMVMGIRDPAAEGNLVTRHTVAEKRRKIEGRILLVEDNLVN
                     QEVTLGMLSVLGCEADVAGNGREGLDAITAREYDLVLMDCQMPVMDGYAATRALRVRE
                     KKTGGKHLIVVALTANAMQGDRDICLAAGMDDYLSKPFTIRKLGETVSKWISAERKDS
                     GVPVEAQAELAVPAKPPISPIDQTVLDGIRELEGDGNKGFLERVINLYLSGASRLVEG
                     VLSAAETGDAESLLRAAHTLKSSSANVGATGLSDLCRKIEEKARRGEPVVAGDPLLSK
                     FEREFRSVREALAAILEGTSS"
     gene            complement(3464..4150)
                     /locus_tag="VF357_02285"
     CDS             complement(3464..4150)
                     /locus_tag="VF357_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX7523002.1"
                     /translation="MNLLSVPRPESVIFDFDGILVDSEPMHHRAFTEVLDPLGMGFPW
                     QEYVETYMGFDDRDAFREAFRAKGIDLDDANLAKLVAAKSRAFQDGLRNGVTSYPGAV
                     PMIESLHAAGRPLALCSGALRSDIDPILAQLDVARRFDVIISADDVRRSKPDPESYAL
                     AFAKLSERRATLLTVPGKSVAVEDTPAGIRSAKGAGLRVLAVTNSYGAGELAEADWIT
                     GSLENVRFEG"
     gene            4351..4917
                     /locus_tag="VF357_02290"
     CDS             4351..4917
                     /locus_tag="VF357_02290"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEX7523003.1"
                     /translation="MTVRMRRKFQDGAVAILFLMGMLCPSALATERIEEGDVKAAFVL
                     NFIKFVEWPASAFHAPEDPIMLSVLGRDPTADSLASLDGKMVSGRRVVVRKVSGLSAL
                     ARCHVLFVGASEKAQLEPILGAVQRWPVLTIADFEGFAGRGGTIGFLRRENRVGFEIN
                     EESARKAGLNVSAKLLYLGKTVPGRREG"
     gene            4919..7378
                     /locus_tag="VF357_02295"
     CDS             4919..7378
                     /locus_tag="VF357_02295"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7523004.1"
                     /translation="MIPSLRDAPIRKKLVAIILLTSGLVLAGSSVVFVVNEAVSFRSD
                     ARKALESTASVIGNNSVAAVMFMDAKVADESLSGLRTNESILSAYLLTGDNQILAAYV
                     SPKAAPGGLPLGIGFDDGMRKVSAEELEALRRDAGTWNFRAIDAVSPIMIGGQRVGTV
                     VLRASFQELGNRMERYLLLCVLVLFGAFLGAYLISRRLAPLISGPIVDLARTMRLVSK
                     EQDFSIRCVERGGNEIGTLIEGFNEMLGQIQDRDRKLLAHREELEAEVARRTAELVQA
                     KDAAEAASKAKSEFLAKMSHEIRTPMNGILGMTDILLQEGLQGEQRRSMEIVQRSGET
                     LLEIINDILDFSRIESGRMELDDTPFDLGGIVEEVMELLAERTQSRGIELASQVDPDV
                     PSPLRGDPGRLRQILFNLVGNAIKFTEQGEVIVRVSLGSRRDGTVGVRFSVRDTGIGI
                     PVQAQAKIFDSFTQADGTTTRKFGGTGLGLTISKQLVGMMGGTIRVVSAPGKGAEFIF
                     TIRLREAAKSSLTPPTPRPDLKGLKVLVVDDNRTNREILEKQLHVWGMSSGGAGGGDE
                     ALSILRMASSEGAPFDLAILDYHMPDIDGLQLAGLIKADSSLSGIRLIMLSSVGIRGD
                     GKKARETGVSGYLTKPVRRDVLYESIAAVMGILDPAAEGKLVTRHTVAGTQKKIDGRI
                     LLVEDNLVNQEVTLGMLSVHGCRADVAENGQEALDAITVGEYDLVLMDCQMPVMDGFA
                     ATRALRAREKETGGKHLTVVALTANAMQGDRDSCLAAGMDDYVSKPFTIQRVGDILAK
                     WLPAGGKPAPPGADTTPPEPS"
     gene            7555..>9177
                     /locus_tag="VF357_02300"
     CDS             7555..>9177
                     /locus_tag="VF357_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7523005.1"
                     /translation="MAAASDLTEMSIEELMNINVYSVSRRLQRLQDSAAAVFVITGED
                     IRRSGATSIPEALRMAPGIDVARVDGNKWAITSRGFNSLFANKLLVLIDGRSVYTPLF
                     SGVFWDVQDTLLEDVDRIEVIRGPGAALWGANAVNGIINILTKTAKQSQGGLLTAGAG
                     NVEKGFGGGRVGGKRGDALFYKAYAKYFNRDGGLDARGNEGPDAWNLLQGGFRVEWEP
                     SERDQFNVQGDIYGGKAGQKVTASDLSSPSFTRTFDERSDVSGGNVLGRWTRTLTNGS
                     EYKLQAYYDRTGNQMAETDMTQDTFDVDFQHRFLLGERQEILWGLGARMWQDRIGGGT
                     LVVLGPVRSNDRTFSGFVQDEITLIPQRVRLTLGTKVEYNRNYDFEFQPNARLIWTPT
                     PRQSLWAAVSRAVRTPSRFERNVSFDAGILPAGAPGNPSPAPLVITVRGNPEFDSEKL
                     IAYETGYRLRPTDRLSLDAALFFNKYDKLRSASSGTPFLDQSPFPHLVAPQTLVNEAD
                     GETFGGELAVDWRPIDAWRLQATYAYLRIQLQQ"
BASE COUNT         1722 a         2824 c         2981 g         1650 t
ORIGIN      
        1 tccttgtcgt tcgcgaagat cccggcttcc ttcagaccca cgagccagga gatggtggtg
       61 gcgtgcgagg agggatccgg catgacgatc ttgtcgcgcc atttcgggtt ggccagatcc
      121 ttgtaggccg tggggacgtc cccgggtttg accaggttct tgttgtagat caaggcgaca
      181 tactcgatgc cgaactggac gatggtgtcg tcctgtctcg cccagccggg gtacccggcg
      241 gcggcgggcg acctgtacgc ggcgagcaca cccttctcct tcagcgcctg caggatcggc
      301 agtggaccct ggagcaggtc ggcttccagt ttgccggcct cgaactccgt caacacggtt
      361 gcgagatact tggtagtgga gatgcgcgtg taaacgcctt ttaccccctt ggcggcggta
      421 aacgcgtcca tgatcggctc caccgcggtg atattggcgt agaaattggc ctttccttcc
      481 ttcttcgcca aggcgggatc ggacgcgaaa gcgttcggga ccaggacgac gctcaacaac
      541 agaagagcca gtcttttcat gatcgtttct cctctccctc cgtgaacctg ccgcaccgcc
      601 ccgatttact cacaagggac acaaatatcg cctgccggcg ccccctgtca atcgcaaaca
      661 caagcgtgtc gaagttaagt atttcctgtt tgcgccgatg agttgtaagg gattccgcat
      721 ttttggacca ggagaaaagg tcaggaacgg cacaggggac gaaccggctg cgccggtgct
      781 gcccaaataa gggagatccg gcttgacgag gattttgttt ccagaggggg gggataccaa
      841 gcaggcgctg cgtcagcagc gctatctgat ggcggccggc actgccgttg tggagatatt
      901 cctcttggcg ctgtgctggc accaggggat tttcgccctc atccacttcc tgcgggccgt
      961 cgccgccctc acgttcatca acgtgctttt cttcattatg ttccgcaccg gctggaaccg
     1021 ccgcttcacg gaccccagcc tgaccttgcc gcagatcatt gcctccttcc tggtcacaac
     1081 ctatcttgtt tattttgccc gcgaagcgcg cgcaatattt cttctgttct acctggttac
     1141 tttcctgttc ggggtgttcc aactccgccc ccggcagtta tatggcatcg cattcgtggc
     1201 gattgcgtcc catgtgtcgg tgatcacctt gctgtggcgg ttccgcccgg agaccatgga
     1261 tctaggcatc gaactggcgc aatggatcgt gatggctgcg gtgatatttt ggtttgccag
     1321 catgggcgtc tacacctcca acctgaggaa caagctgcgc agcagtaagg tggaactgga
     1381 aaatgccctc agatccgttc gtgacagcgc cgtggacctg caatcggcaa aagagagggc
     1441 ggagaaagcg aaccggacca agagtgaatt cctcgcgaac atgagccacg agatccgcac
     1501 ccccatgaac ggaatcctgg ggatgacgga gattcttctt catggggacc tgccgtccga
     1561 gtaccgccgg gacgtggaac ttgtgcacaa ctccggggag atactgttgg gtatcatcaa
     1621 caacatcctg gactactccc ggatcgaggc cggcaagatg gaactggacg acatcccgtt
     1681 cgatcttgca ggggtcgtcg atgagataat gaaactcctc tcgatgcgtg cccagtccaa
     1741 ggaggtcgag ctggtttcgc aggtttcccc ggagatcccc tccgccctcc gtggggaccc
     1801 cggccggctc cggcagatcc tggtcaacct ggtgggaaac gccgtcaaat tcacggagag
     1861 gggagaggtc gtcgtccggg cgtccctcga ttcccgggac gacggggcgg tgaccgtgcg
     1921 gttctccgtc cgcgacacgg ggatcggcat tctcccggag gcccatgcga agatcttcga
     1981 ctccttcacg caggccgacg gatccaccac gcggaagttc ggcggcaccg ggctcgggtt
     2041 gaccatctcc cggcaactcg ttgagatgat gggcggaacg atccgggtgg aaagcgcgcc
     2101 cggcaagggg tccgatttca ttttcaccat cccgctccac acgatggagg aggcggacct
     2161 ccggccttcg attccccggc ccagtctcaa gggcctgaag gtgctggtcg tggacgacaa
     2221 ccggacgaac cgcgagattc tcgagaagca gttgcacgat tgggcgagtc aaagccgcgg
     2281 ggccggcagt ggagacgagg cgctctcgct cctgcgcgtg gcggcagcgg agggcgtctc
     2341 cttcgacctg gcgatcctcg actaccacat gcccgacatc gacggttttc aactggccgg
     2401 gttgatcaag tcggaccctt ccctctccgg cgtccgtctc ctgatgctct cctccgtcgg
     2461 gatccgcggg gacggacgga aggcgcggga aaccgggatt tccggatacc tgagcaagcc
     2521 ggtccgacga gaggatctgt acgaaagcat cgccatggtg atggggatcc gggacccggc
     2581 ggcggaagga aacctggtga cccgccacac cgtcgcggaa aagcgcagga agatcgaagg
     2641 gcgaatcctg ctggtcgagg acaaccttgt aaaccaggag gtcaccctgg ggatgctctc
     2701 tgtcctcggg tgcgaagccg atgttgcggg aaacggacgg gaaggcctcg acgcgatcac
     2761 cgcccgggag tacgacctcg tcctgatgga ctgccagatg ccggtcatgg acggctatgc
     2821 ggcaacccgg gccctccgcg tccgcgaaaa gaaaacgggc gggaagcacc tgatcgtggt
     2881 agccctcacg gccaatgcga tgcaggggga cagggatatc tgcctggccg ccgggatgga
     2941 cgattacctc agcaaacctt tcacgatccg gaaactcggg gaaacggtat cgaagtggat
     3001 ctccgcggaa cggaaggact ccggcgtccc cgtcgaagcg caggcggaat tggctgttcc
     3061 ggcgaagccc ccgatctccc cgatcgacca gaccgtactc gacgggatcc gggaactcga
     3121 gggagatggg aacaaagggt tcctcgaacg ggtcatcaac ttgtacctct ccggtgcctc
     3181 ccggctggtg gagggagtcc tttccgcggc cgaaacgggg gatgcggaat cccttcttcg
     3241 ggccgcccat accctgaaat ccagcagcgc gaacgtgggc gcgaccggtc tttcggacct
     3301 ctgccggaag atcgaagaga aggcccggcg gggcgaaccg gtcgtcgccg gggaccctct
     3361 cctgtcgaaa ttcgaaaggg agttccggtc ggtaagggaa gcactggcgg ccatcctgga
     3421 ggggacctcg tcctgacgga gccgatcgga acctctcccc ccgtcatccc tcgaagcgga
     3481 cgttctccag cgaaccggtg atccagtcgg cttcggcgag ttctcccgca ccgtagctgt
     3541 tggtgacggc gagcacccgg agtcccgccc ccttcgcgga ccggatcccg gcgggggtgt
     3601 cctcgacggc gaccgatttt cccgggaccg tcagcaacgt ggcgcgcctc tccgacaatt
     3661 tcgcgaaggc gagcgcatag ctttccgggt cgggcttgct cctgcgcacg tcgtcggccg
     3721 aaatgatcac gtcgaagcgg cgggcgacgt ccagttgcgc gaggatcggg tcgatgtcgg
     3781 agcgaagggc gccgctgcac agggccaggg gccgtcctgc cgcgtgcagg gactcgatca
     3841 tggggacggc tcccgggtag gaggtcaccc cgttgcggag gccgtcctgg aatgcccggg
     3901 acttcgcggc gacgagtttt gcgaggttcg cgtcgtcaag gtcgatcccc ttcgcccgga
     3961 acgcctcccg gaaggcgtcg cggtcgtcga accccatgta ggtctcgacg tactcttgcc
     4021 atgggaaccc catcccgagg ggatcgagaa cctccgtgaa cgcccggtga tgcatcggtt
     4081 cgctgtcgac gagaatcccg tcgaaatcga agatgaccga ttcgggcctt gggaccgaaa
     4141 ggagattcat cggcgcgccg ggattctcat cacaaaagga aatatacccc ggatccgccc
     4201 ctcggaggaa ccgggcgaac ctttcttccg ggatacgatc ggtgaacctc ccgtaaagtt
     4261 ttcccccccg aatgccgata cgtatatgtt ggcacgacga tgggtcggaa tgagaaagac
     4321 gaaggattcc ggagaggttg attgcagcga atgaccgtga ggatgcgccg gaagttccag
     4381 gatggggcag tggcaatcct cttcctcatg gggatgctct gcccgtcggc gctggccacg
     4441 gaaaggatcg aggagggcga cgtaaaggcg gccttcgtcc tcaacttcat caagttcgtg
     4501 gaatggccgg cctccgcctt ccacgccccg gaggacccca tcatgctctc cgtcctcggg
     4561 cgtgatccca ccgcggactc cctcgcgtcc ctcgacggga agatggtatc ggggaggcgg
     4621 gtggttgtcc ggaaggtttc gggtttgtcg gcgctggcgc ggtgccacgt gctcttcgtc
     4681 ggcgcttccg agaaggcgca gctggaacca atccttggcg ccgtccagcg atggcccgtt
     4741 ctgacgatcg ccgatttcga ggggttcgcg ggcaggggcg gaacgatcgg gttcctccgg
     4801 cgggagaacc gcgtcggttt cgagatcaac gaggagtccg cgcggaaggc ggggctgaat
     4861 gtcagcgcga agctcctcta cctcggcaag accgtcccgg gccgcaggga gggatgagat
     4921 gatcccatcg ttgagggacg ctcccatccg gaaaaagctc gttgccatca tcctgctgac
     4981 cagcggcctc gtcctcgccg ggtcctcggt cgtgttcgtg gtcaacgagg cggtgtcctt
     5041 ccgttcggat gcccggaagg ccctcgaatc gaccgcgagc gtaatcggca acaacagcgt
     5101 cgccgccgtc atgttcatgg acgcgaaggt ggcggacgaa tcgctttccg gtctccgcac
     5161 gaacgaatcg atcctctctg cctacctgct cacgggcgac aaccagatcc tggccgccta
     5221 cgtctccccg aaggcggccc cggggggcct tcccctcggg atcgggttcg acgacggcat
     5281 gcggaaggtg tccgccgagg aactggaagc gctccggcgg gatgccggga cctggaattt
     5341 ccgcgccatc gacgcggttt cgcccatcat gatcgggggg cagcgtgtcg gcacggtggt
     5401 tctgcgggcc tcgttccagg agctcgggaa ccgcatggag cggtacctcc tcctctgcgt
     5461 cctggtcctg ttcggcgcct tcctcggcgc gtacctgatc tcccggcgtc ttgcccccct
     5521 catttccggc ccgatcgtgg atctggcccg cacgatgcgc ctggtgtcga aggagcagga
     5581 cttctcgatc cgctgcgtgg agcggggggg aaacgagatc ggaacgctga tcgaagggtt
     5641 caacgaaatg ctcgggcaga tccaggaccg ggacaggaag ctccttgccc accgggagga
     5701 gctcgaggcg gaggtggccc gcaggaccgc cgaactcgtc caggcgaagg acgcggcgga
     5761 agccgccagc aaggcgaaat ccgaattcct tgcgaaaatg agccacgaga tccgcacccc
     5821 gatgaacggg atcctgggga tgacggacat tctcctccag gaaggcctgc agggcgaaca
     5881 acgccggtcc atggagatcg tccagcgctc cggggaaacg ctcctagaga tcatcaacga
     5941 catcctcgat ttctcccgga tcgagtccgg caggatggag ctggacgaca cccccttcga
     6001 tctcggaggg atcgtggagg aagtgatgga gctcctcgcg gagcggaccc agtccagggg
     6061 gatcgagctg gcttctcaag tcgatccgga cgttccatct cctcttcgtg gagaccccgg
     6121 ccggctccgg cagatccttt tcaacctggt cggaaacgcg atcaagttca cggagcaggg
     6181 tgaggttatc gtccgtgtgt ccctcggttc ccggagggac gggacggtgg gagtaaggtt
     6241 ctccgtccgc gacacgggga tcggcatccc tgtgcaggcc caggcgaaaa tcttcgactc
     6301 cttcacccag gcggacggca ccaccacgcg gaaattcggg ggcacggggc tcgggttgac
     6361 catctccaag caactcgtcg gcatgatggg gggaaccatc cgggtcgtga gcgcacccgg
     6421 caagggggcc gaattcattt tcactatccg gctccgggag gcggcgaagt cgagcctcac
     6481 gccgccgacc ccccgcccgg acctcaaagg attgaaggtg cttgtcgtgg acgacaaccg
     6541 gacgaaccgg gagatcctcg agaagcagtt gcacgtctgg ggaatgagca gtggcggagc
     6601 cggcgggggc gacgaggcgc tctcgatcct gcgcatggcc tcatcggaag gggctccctt
     6661 cgatcttgcg atcctcgatt accacatgcc cgacatcgac ggcctgcaac tggcggggct
     6721 gatcaaggcg gactcctccc tctccggcat ccgcctcatc atgctctctt ccgtcgggat
     6781 ccggggggac gggaagaagg cgcgggagac gggggtctcc ggatacctga cgaagccggt
     6841 ccgacgggat gtcctctacg agagcatcgc cgccgtgatg gggatcctgg acccggcggc
     6901 ggaagggaag ctggtgaccc gccacaccgt cgccggaacg cagaagaaga tcgacgggag
     6961 aatcctgctg gtcgaagaca acctcgtgaa ccaggaggtc accctcggga tgctctcggt
     7021 ccacggttgc cgggccgacg tcgccgaaaa cggccaggaa gccctcgatg cgatcaccgt
     7081 cggggagtac gatctggtgt tgatggactg ccagatgccg gtcatggacg gcttcgccgc
     7141 cacccgggcc ctgcgcgccc gcgaaaagga aacggggggg aaacatctga ccgtcgtggc
     7201 gctcacggcc aacgcgatgc agggagacag agactcctgt ttggccgccg gcatggacga
     7261 ctacgtgagc aagccgttca caatccagag agttggagac atactcgcga agtggctgcc
     7321 cgcgggcgga aagcccgccc cgcccggtgc cgacacgacg ccgccggaac cgtcgtgatg
     7381 gaacaaagag gtccaaggag tcccgtcatg gacgaaaggg gaaacgtcga tatgcgaagc
     7441 atcaccgttg ccactgcgga aggagccgga gccaggtcct tgcgatggat cctatatgtg
     7501 gcatgtcttg cggcgatgtc gatcccgggc ggcgcctggg ccccgggggt tgcggtggcc
     7561 gctgcatccg accttaccga gatgagcatc gaggaactga tgaacatcaa tgtttactcc
     7621 gtttcccgga ggctccagcg gctccaggac agcgccgccg ccgtcttcgt gatcaccggg
     7681 gaagatatcc ggcggtccgg cgccacgagc atccccgagg cccttcggat ggccccgggg
     7741 atcgatgtcg cccgcgtcga cggcaacaag tgggccatca cgtcccgcgg attcaacagc
     7801 ctgttcgcca acaagctgct ggtgctgatc gacggccgaa gcgtgtacac gccgctcttt
     7861 tccggcgtgt tctgggacgt tcaggacacc ctgctggagg acgtcgaccg gatcgaggtg
     7921 atccggggac ccggggccgc gctgtggggc gccaacgcgg tcaacggaat catcaatatc
     7981 cttacgaaga cggcgaagca gtcccaaggc ggcctgctga ccgccggggc ggggaacgtg
     8041 gagaagggat tcgggggggg ccgggtcggc gggaagcgcg gagatgccct cttctacaag
     8101 gcctacgcaa aatatttcaa ccgggacgga ggcctggacg cccgcggcaa cgagggcccc
     8161 gatgcgtgga accttcttca gggcggtttt cgcgtggagt gggaaccgtc ggaacgcgac
     8221 cagttcaacg tccagggcga catctacgga gggaaggccg gccagaaggt gacggcctcg
     8281 gatctgtcct ccccctcgtt cacccgaacg ttcgacgaac gatcggacgt ctccggcggc
     8341 aacgtgctcg ggaggtggac ccgcacgctg acgaacggtt ccgaatacaa actgcaggca
     8401 tattacgacc ggacgggcaa tcagatggcg gaaaccgata tgacgcagga taccttcgac
     8461 gtcgatttcc agcaccggtt cctcctcggg gaacggcagg agatcctatg gggactcggg
     8521 gcacggatgt ggcaggaccg catcgggggc ggaaccctgg ttgtcctggg tcccgttcgc
     8581 agcaacgatc gcacgttcag cgggttcgtg caggacgaga tcactctgat ccctcaacgc
     8641 gtccggttga cgctggggac gaaagtcgaa tataaccgga attacgattt cgaattccag
     8701 ccgaatgccc ggctgatctg gacgccgact ccgcgccaat cgttgtgggc ggcggtgtcg
     8761 cgcgcggtcc gtaccccctc gcgtttcgaa cggaacgtct ccttcgatgc cggcattctt
     8821 ccggccggcg cccccgggaa cccgtctccc gcgccgctgg tgataaccgt tcgagggaat
     8881 ccggaattcg attccgaaaa actgatcgcc tatgaaacgg gataccggct gcgcccgacc
     8941 gaccggctct ctcttgacgc ggcgctgttt ttcaacaagt acgataaatt gcgctctgcc
     9001 tcctccggaa ccccgttcct ggaccaatcg cccttccccc accttgttgc cccgcaaacg
     9061 cttgtcaacg aagcggacgg cgaaaccttc ggtggggaac tggcggtcga ctggcgcccg
     9121 atcgatgcgt ggcgcctcca ggcaacctac gcctacctgc ggatccagct gcaacaa
//
LOCUS       DASVJL010000119         2607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_112516, whole genome shotgun sequence.
ACCESSION   DASVJL010000119 DASVJL010000000
VERSION     DASVJL010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112516"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VF357_02305"
     CDS             complement(<1..311)
                     /locus_tag="VF357_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7523006.1"
                     /translation="MKLAKRVGKIQPSPTLAITNKAKQMKAQGIDVVGFGAGEPDFDT
                     PDHIKAVAKKALDDGYTKYTPVPGSPELKDAIIAKLKRDSGLEYKRENIIVSLGAKHS
                     I"
     gene            complement(308..802)
                     /gene="coaD"
                     /locus_tag="VF357_02310"
     CDS             complement(308..802)
                     /gene="coaD"
                     /locus_tag="VF357_02310"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX7523007.1"
                     /translation="MRTVAVYPGSFDPPTNGHLDIIERSSRVFSRVIVAVAVNLRKNA
                     FFTPAERVRMLKRLTSTMGNVEVTSFRGLLVDFARVQEAHILVRGIRAISDFEYEYQM
                     AHMNRKLDQEIDTVIMMTGERFSAISSNIVKEIAQLGGKIDDLVPPLVRDILFSKLKG
                     AKRK"
     gene            complement(799..1395)
                     /gene="rsmD"
                     /locus_tag="VF357_02315"
     CDS             complement(799..1395)
                     /gene="rsmD"
                     /locus_tag="VF357_02315"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX7523008.1"
                     /translation="MRVVAGKWGGRTIRAPRGTSVRPTTDRVREAVFAILGDDVEGTV
                     VLDLFAGTGAMAIESLSRGASGAVLVESSPVALVALRANLAALEAEEAVCLPLDYREA
                     VRRLSAKGRTFDLVFLDPPYGKGLVGRSAGLLSRAGILAPGAVVVAERASRDPGDALP
                     AEWRERADRRYGDTRITLYDIPDPRGQAPPGGQKQEKR"
     gene            complement(1404..2492)
                     /locus_tag="VF357_02320"
     CDS             complement(1404..2492)
                     /locus_tag="VF357_02320"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX7523009.1"
                     /translation="MDYRSIKRFVPENAAKEIPPGNPACPVNGRDVYRALAPHKTIVM
                     ACNIRIPLVLPGIMRAAKELDAMVAFELAKSEGDLKGGYTAMPPEIFVRTIIAAAKRA
                     EFDTPFVIHGDHITVKNSSETEVEGARALIAAEIAAGYTSFAIDASFNEIPDNARITA
                     SLAGPIAERKLGLEVEVGEIKSVGTEAHLSTVDEAVDLLERLTAAGVHPDLLAFNNGS
                     KHGNYLEGEKISIDLDRTGEIYRAIHDRFGVSIAQHGITGTPLHLIGKFAEYGIRKGN
                     VGTQWQNIAHAGLPPELMGRMRDWAKEAGKDIKFATKPFKAEIDAVPASFASMIEKAA
                     YEEAKRLFQAFRAADTAKIVAGALAGQA"
BASE COUNT          414 a          895 c          799 g          499 t
ORIGIN      
        1 tagatcgagt gcttcgcgcc gagggagacg atgatgttct cccgcttgta ttcgaggccg
       61 ctgtcccgct tgagcttggc gatgatggcg tccttgagct ccggcgatcc ggggaccggg
      121 gtgtacttcg tatacccgtc gtccagcgcc ttcttggcga cggccttgat gtggtccggc
      181 gtgtcgaaat cgggctcccc ggccccgaag ccgaccacgt cgatcccctg cgccttcatc
      241 tgcttcgcct tgttggtgat cgccagcgtc ggtgacggct ggatctttcc cactctcttc
      301 gcgagcttca ttttctcttc gctcctttca gcttactgaa caagatgtcc cgcaccaaag
      361 gaggtacgag gtcgtcgatc ttaccaccga gctgggcgat ctccttcacg atgttcgagc
      421 tgatcgcgga gaaacgctcc ccggtcatca tgatcaccgt gtcgatctcc tggtccagtt
      481 tccggttcat gtgcgccatc tggtactcgt actcgaagtc cgagatcgcc cgtatccccc
      541 gcaccaggat gtgcgcctcc tgcacgcggg cgaaatccac cagcaggccg cggaacgacg
      601 tgacctcgac gttccccatc gtggaggtca atcgcttcag catccgcacc cgttccgcgg
      661 gggtgaagaa ggcgtttttc cgaaggttca ccgccaccgc gacaatcacg cgcgagaaga
      721 cccgggagga tcgctcgatg atgtccaggt gtccgttcgt ggggggatcg aacgaaccgg
      781 gatagacggc cacggtcctc atcgtttctc ctgtttctgc ccgcccggcg gcgcctgccc
      841 gcggggatcg gggatgtcgt acaaggtgat ccgggtatct ccgtacctgc ggtcggcacg
      901 ctccctccac tccgccggaa gcgcatcccc cggatcccgg gaggcccgct ccgccaccac
      961 gacggcgccg ggagcgagaa tgccggcgcg ggaaagcagc cccgccgacc ggccgaccag
     1021 ccccttgcca tacggggggt cgaggaacac gagatcgaac gtcctcccct tcgcggagag
     1081 cctccggacc gcctcccggt agtcgagggg gaggcagacc gcctcttccg cctccagcgc
     1141 cgcgagattc gccctcagcg cgacgagcgc caccggtgaa gactccacga gcaccgcccc
     1201 ggacgccccg cgcgagagcg attcgatcgc catcgcgccc gtcccggcga aaaggtcgag
     1261 cacgacggtt ccctccacgt cgtccccgag aatcgcgaac acggcctcgc ggacccggtc
     1321 ggtggtcggc cgcacggagg tcccgcgtgg ggcgcgaatc gttcgccccc cccacttccc
     1381 cgcgacgacg cgcaccttcg gtgctaagcc tgcccggcaa gcgctccggc gacgatcttc
     1441 gccgtatcgg cggcccggaa cgcctggaag agccgcttcg cctcttcgta cgccgccttc
     1501 tcgatcatcg aagcgaacga agcggggacg gcgtcgatct cggccttgaa cggcttggtg
     1561 gcgaacttga tatccttgcc ggcctccttg gcccagtcgc gcatcctccc catcaattcc
     1621 ggggggagcc ccgcgtgggc gatgttctgc cactgggtgc cgacgttccc cttgcggatc
     1681 ccgtattcgg cgaacttccc gataaggtgg agcggcgtcc cggtgatgcc gtgctgggcg
     1741 atggagacgc cgaagcggtc gtggattgcc cgatagatct cgccggtgcg atcgaggtcg
     1801 atggagatct tctccccttc caggtagttg ccgtgcttgg acccgttgtt gaacgccagc
     1861 aggtccggat ggacccccgc cgcggtcagc cgctccagga gatccacggc ctcatcaacg
     1921 gtcgacaggt gcgcctcggt ccccaccgac ttgatctccc ccacctccac ctcgaggccg
     1981 agcttgcgct ccgcgatggg tcccgcgaga ctggcggtga tccgcgcatt gtcgggaatc
     2041 tcgttgaacg acgcgtcgat ggcgaagctc gtatacccgg cggcgatctc cgcggcgatc
     2101 agcgcgcggg ccccctcgac ctcggtttcc gaactgttct tcacggtgat gtggtcgccg
     2161 tggatgacga acggggtgtc gaactccgcc ctcttcgccg ccgcgatgat cgtgcggacg
     2221 aagatctcgg gaggcatcgc cgtgtagccg cccttcaaat ccccttccga cttggccagc
     2281 tcgaacgcga ccatggcgtc cagttccttc gccgccctca tgatccccgg gaggacgagg
     2341 gggatccgga tgttgcacgc catgacgatc gtcttgtggg gcgcgagggc gcgatatacg
     2401 tcgcggccgt tgaccgggca cgccggattt cccgggggga tctcctttgc cgcattttcc
     2461 gggacaaacc gcttgatcga cctgtaatcc atgggcacct ccttcaggct ggccccccca
     2521 ttattggaat tttggacccg tacgtcaagg cggttacgga accggcatgt tagactaagc
     2581 gatctatttc acaaatacgg caacgta
//
LOCUS       DASVJL010000120         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_580426, whole genome shotgun sequence.
ACCESSION   DASVJL010000120 DASVJL010000000
VERSION     DASVJL010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580426"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VF357_02325"
     CDS             complement(<1..262)
                     /locus_tag="VF357_02325"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX7523010.1"
                     /translation="MKGPASYPTLPTILVGVVLTLLGGALSAWSPPLADSIEGKVYDS
                     FLRSAPRHPAPGPIAIVDLDEASLERLGQWPWPRYRIARLLER"
     gene            complement(271..894)
                     /locus_tag="VF357_02330"
     CDS             complement(271..894)
                     /locus_tag="VF357_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7523011.1"
                     /translation="MTRRSFVFPIAILLLAAACATPPKPSPPPSRDIIVLLPDDQGKT
                     GSIVVSSAGVERRLDQPGQAVTVEAGSPPGLPTVMSGQEVLAIAGPALAALPKPPARF
                     ILYFEHDSVCLTMESHALLMTVLDAIRARASVDISVVGHTDTVGKKKYNYALSLRRAR
                     TVASFLLGKGVDPSALHVTSHGKDNPLVPTGDQVHEPRNRRVEITVR"
     gene            complement(926..1393)
                     /locus_tag="VF357_02335"
     CDS             complement(926..1393)
                     /locus_tag="VF357_02335"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX7523012.1"
                     /translation="MSKNTVTAALVLSALCLLALAGTAVAEETIGVVRTASGDATVTR
                     GEKTLPATPGLTLLVGDTLGTSRDGSLGVILRDDSSLSIGPGSRLVLQSFLFSPSEGK
                     FDLVARITRGTMAYLSGLIGKLAPEKARFETPTATIGIRGTRFAVKVGEPSSR"
     gene            complement(1541..>2147)
                     /locus_tag="VF357_02340"
     CDS             complement(1541..>2147)
                     /locus_tag="VF357_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX7523013.1"
                     /translation="IVISNPLDAMVTLCQKITGFPNNRIIGQAGVLDSARFAAFIAWE
                     LGVSVRDVTAMTLGGHGDDMVPLIRYASVAGIPVMELLERKYGNAAKAKEVMEAMVNR
                     TRKAGGEVVALLKTGSAFFSPASAAIAMVESILKDQKRVLPCCVYLNGEFGVKGYFVG
                     VPTVLGAGGVEKIIEFKLNAEEQAMMDKSVAAVKELVGTLK"
BASE COUNT          354 a          687 c          758 g          348 t
ORIGIN      
        1 tccgctcgag gaggcgggcg atccggtacc gcggccacgg ccactggccg aggcgttcga
       61 gactcgcctc gtcgagatcg acgatcgcga tcgggcctgg cgccgggtgt cgcggggcgg
      121 accggagaaa agagtcgtag accttcccct cgatggaatc ggccagcggc ggggaccagg
      181 cggagagggc ccctccgagg agggtgagga cgacgcccac gaggatcgtt ggaagggtgg
      241 ggtagctcgc cgggcctttc accgcgccgg ttaccggacc gtgatctcca ctcggcggtt
      301 ccgcggctcg tggacctggt cgccggtggg gacgagcggg ttatccttcc cgtgggacgt
      361 gacgtggagt gcggagggat cgaccccttt cccgagaagg aaggaggcaa ccgtcctggc
      421 gcgtctcagg gaaagggcgt agttgtactt tttctttccc accgtgtcgg tgtgacccac
      481 cacgctgatg tccaccgagg cgcgggcgcg gatcgcatcc agcactgtca tcagcaatgc
      541 atgcgactcc atcgttaggc agaccgagtc gtgctcgaag tagaggatga accgcgcagg
      601 cggtttcggg agggccgcga gggcggggcc cgcgatcgcg agcacttcct gcccggacat
      661 cacggtgggg agtccgggag gggaccccgc ctcgaccgtt accgcttgcc ccggctggtc
      721 aaggcgccgc tcgacccccg cgctcgagac gacgatggat ccggtcttcc cctggtcgtc
      781 ggggaggaga acgatgatgt cacgcgaggg agggggagac ggcttcggcg gcgtggcgca
      841 ggcggccgcc aggaggagga tcgcgatcgg aaaaacgaac gatcgccggg tcacgcggga
      901 cccggattcc ggggggcgat cgcgttcatc gagaggaagg ttcccccacc ttcacggcga
      961 agcgcgttcc ccggatgccg atggtggccg tgggggtttc gaatcgtgct ttctccgggg
     1021 ccagcttgcc gatcaggccc gagaggtaag ccatcgtccc ccgggtgatg cgggcgacaa
     1081 ggtcgaactt tccctccgag ggcgagaaca ggaagctctg gaggacgaga cggctccccg
     1141 ggccgatgga gagggaggag tcgtcacgga ggatgacgcc gagcgatccg tcccgactgg
     1201 tgccgagggt gtcccccacc aggagggtca accccggggt ggccggaagc gttttttccc
     1261 cgcgggtgac ggtggcgtcg cccgacgcgg ttcgcaccac gccgatcgtc tcctcggcga
     1321 ctgccgtacc ggcaagggcc aggaggcaca gggcgctcag aacgagagcg gcggtcacgg
     1381 tgttcttcga catattcccc ctccggattt ccggcgttac gctcccccca tttatacccc
     1441 tctccccccg gggagtcgag atcctgtcgt tccgggacgg cgccggccgg gaggggggac
     1501 cccgtcccgg ccggcgcgca tcgttcgatg cgtggatacg ctacttgaga gtgccgacca
     1561 attccttcac ggcagcgacc gacttgtcca tcatcgcctg ctcctcggcg ttgagcttga
     1621 actcgatgat cttctcgacc ccgcctgcgc ccaggacggt cggaacgccc acgaagtacc
     1681 ccttcacgcc aaactcgccg ttcaggtaga cacagcacgg caggacccgc ttctggtcct
     1741 tcaggatcga ctcgaccatg gcgatcgccg cagacgccgg ggagaagaag gcggaaccgg
     1801 tcttcagcag ggcgaccact tcgccgccgg ccttccgggt gcggttcacc atcgcctcca
     1861 tgacttcctt cgccttcgcg gcgttgccgt atttccgctc caggagctcc atgacgggga
     1921 tcccggccac ggaggcgtac cggatgaggg gaaccatgtc gtcgccgtga ccgcccaggg
     1981 tcatcgccgt cacgtcccgc acggaaacgc cgagctccca ggcgatgaag gcggcgaagc
     2041 gggccgaatc gaggacgccg gcctgcccga tgatccggtt gttggggaac ccggtgatct
     2101 tctggcagag ggtcaccatc gcgtcgagcg ggttggagat gacgatg
//
LOCUS       DASVJL010000121         9649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1519575, whole genome shotgun sequence.
ACCESSION   DASVJL010000121 DASVJL010000000
VERSION     DASVJL010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519575"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /gene="recG"
                     /locus_tag="VF357_02345"
     CDS             <1..1995
                     /gene="recG"
                     /locus_tag="VF357_02345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX7523014.1"
                     /translation="KDYEDRRKVVPIRELKAGMTVPVRGKILSVQGGGKGFRPAQVLE
                     VVIADGTGRLSAKWFRFHPSLAERFRVGDSVTLCGSARWFRFFPEMHHPEILAEEDAV
                     DPVHSGRIVPVYPEVEGIPPRVLRKIQWEVVQRHASLEIECFPSSILARVGIPPLQES
                     LASIHFPRDDADADRFREFSSPQQRRLIFGELFALQWALALRRAGTEQEEAIPLPWDR
                     EIVDEIKRRLPFDLTGAQRRVVNEILKDLGKPHPMHRLLQGDVGSGKTIVAWIAAMVA
                     WRHGVQAAVMAPTEILAEQHYRRFLERSEGLPVRVALLSAALPTKEREAVRKQIREGE
                     ADIVVGTHALIQESVAFRNLALGVIDEQHRFGVLQRASLRRKGRISPHLLVMTATPIP
                     RTLAITLYGDLDVSILDEMPRGRIPVRTKVVPEDGRRKVFEEIRREIAQGGRVYVVLP
                     LVEESDKVALRDATRTAERLREAFPDVGVGLLHGRMKAAEKEEIMGRFKGGDLQLLVS
                     TTVVEVGVDVPEATVMVVEHSERFGLSQLHQLRGRVGRGSRPSSCFLMTGREKGEEAA
                     ARLSVMEETVDGFRIAEEDLRIRGPGDFAGVRQSGIPDLVFADLVRDASILRLAREIV
                     GELHRDDPGLSSPDHLRIRRFLEMRTTASGGGDPAPSSRD"
     gene            2068..2928
                     /locus_tag="VF357_02350"
     CDS             2068..2928
                     /locus_tag="VF357_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523015.1"
                     /translation="MSGFGLSGFTFCRNAVALGYPLRESIQSILPIVDEFVVALGPSD
                     DGTGELIERIGSPKIRIVETAWDENQMTDARVLSTQATFALSCCRFPWAFHLQADEVI
                     HEADLPGLKALMLQHLENRQALGFMFRFIHFVLDYRSINPWAFRREVRIVRNDGQLRS
                     AGDSCGFARKSDGLYLGKKLLGREVLWTGARVFHYSSVMPRSALEKKISSMSRIRHGD
                     PPAKSLEKITRDGIASLERQYSIMKEYRGSHPSVMQERIAAAERFRPRVNRWLNWRFY
                     LNLFTHGFKG"
     gene            complement(2941..3174)
                     /locus_tag="VF357_02355"
     CDS             complement(2941..3174)
                     /locus_tag="VF357_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523016.1"
                     /translation="MDGTKAIRKKLGNIEGQLKKRVRSVEKDLTTLGKKLEKKETEVK
                     KLKEKLTSKFVKGVKKNVKKAKKTVAKRLSNIL"
     gene            complement(3212..3805)
                     /locus_tag="VF357_02360"
     CDS             complement(3212..3805)
                     /locus_tag="VF357_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HEX7523017.1"
                     /translation="MQNRTAFTVLCCILAATGAGCASSRADFYTLSPSARAAPVTAGY
                     SVAVGPVSVPEIVDRPQIVVRTGPNQVFIDEFHRWGSPLRDDIARGIAGNLSALLGAP
                     QVSVFPHSTSSAAKYRAAVDVMVFDSTPGDAAVLEAVWVVRRAGDGAVRSGRTLVREP
                     VPGVGYAALVSAHCIALEKLSEDIAEAIRGLENEERQ"
     gene            complement(3805..5538)
                     /locus_tag="VF357_02365"
     CDS             complement(3805..5538)
                     /locus_tag="VF357_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX7523018.1"
                     /translation="MSDNSDFTNVPQAESIPKSRARLSIVWIIPILAAAVGVGIAFQQ
                     YMSEGPTILIAFRSGEGVEAGKTSIKYKDIEIGKVTGVNLSEDYSRILITAKMEKSAE
                     GLLVKGARFWIVKPRVTLSGVSGIGTLLSGNYIRFEPGKSTEKGHAFVGLEVPPPATS
                     GLPGREFVLRSDTLGSLGIGSPVYYRRLSVGQVISYGLVENGKSVDIRIFLEAPFDRF
                     VTSNARFWEESGIDVSVGTSGLAVKTESLLSLLVGGIAFETPPSPTGNNPVVDNAVFP
                     LFHDRVTALSPHEVDAERYALYFHGSLRGLSVGAPVDFLGLPVGEVTGVFLAYVPGSQ
                     SIHTRVEIVTYQHRFPVPQGMQGAAAESPTPIKGRLEFMQRQVVEKGLRAQLRSASLI
                     SGQMYVALDYFPDAPKALIDWNRKPPAFPVVPGEMANLQASLKSLLVKLDKVPVEEIG
                     NDARKAIAALDRTLALLEGETLPEAKKTLGTLDRTLQSANRTLERVDGELVPEAKKTL
                     EELRRAVSSAERMLGNMDNTFLGPDAPAQQDLRDAMREISRAAGAVRALADYLERNPG
                     ALIRGKTQEKP"
     gene            complement(5531..6172)
                     /locus_tag="VF357_02370"
     CDS             complement(5531..6172)
                     /locus_tag="VF357_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEX7523019.1"
                     /translation="MPRAPLTGAQAGVVLCGACGLLSQPAGPEEPGNCPRCGEPTVLR
                     HRDPIQRTWALIVAAAICYLPANVLPVMTTSTLKSVVPTTIIGGVVRLYADGSWVLAL
                     IVLVASVMIPLGKLVALSYLLITVQLRSSRSRRERVRLYRMLKAIGRWSMLDVFVATF
                     SVALVQLWPFMSVVPGSGVLFFTAVVILTILAADTFDPRLIWDSGGEKRGEDV"
     gene            complement(6154..6798)
                     /locus_tag="VF357_02375"
     CDS             complement(6154..6798)
                     /locus_tag="VF357_02375"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEX7523020.1"
                     /translation="MPVDIVACPDCDLLQRMPSLPPGGRACCARCGLALVSNKPGSLD
                     RSMALSVAALIAYLLANLEPLMRVSISGRTSSTTILGGVQAMWLQDEKVTAVLVALFV
                     VVAPALDIVFMLAISLASRRPPAPRWVGAGLRWAERSGVWSMVDVMMLGILVSLVKIA
                     SLATVEPGIGIFAVGALVFLLAGMSANFEPREIWPRVRWENGEWPETGRCPAPR"
     gene            7396..8382
                     /locus_tag="VF357_02380"
     CDS             7396..8382
                     /locus_tag="VF357_02380"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7523021.1"
                     /translation="MVRIALGKSDLMVSRMGLGCMGMSEFYGPGDEAESVRTIHRALE
                     LGINFLDTADIYGMGRNEELVGKALRDRRNQAVLATKFGNVRGKDGSWQGVNGKPDYV
                     RSCCEASLRSLGVDVIDLYYQHRVDPETPIEETVGAMADLVRQGKVRYLGLSEAAPVT
                     IRRACAVHPITALQTEYSLWTRDPEAEVLPTCRENGIAFVAYSPLGRGIFSGAIRSLD
                     NLAEGDYRRNSPRFSGENLSHNLSLVDRLTEMATGKKCRPAQLALAWLLSRGQDIFPI
                     PGTKHVNRLEENVGALQVPLTPEEVTRLDTAFPAGAAAGNRYPEQAMKALHR"
     gene            complement(8480..8782)
                     /locus_tag="VF357_02385"
     CDS             complement(8480..8782)
                     /locus_tag="VF357_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HEX7523022.1"
                     /translation="MAIVFVTIAPLGTASSSLSRYVAGVERILRKSGLKHELTAMGSI
                     VEGDLDAILPVLREMHENPFSQGAVRVSTLIKIDDRRDKAASMDAKIRSVKEKLGE"
     gene            8978..>9649
                     /locus_tag="VF357_02390"
     CDS             8978..>9649
                     /locus_tag="VF357_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7523023.1"
                     /translation="MEEFARIQRLPPYVFAVVVDLKSRMRHAGEDIIDLGMGNPDLPT
                     PKHIVDKLVEAVRNPRNHRYSLSRGIPKLRLAICNWYERKYNVDLDPETEAIATIGAK
                     EGLSHLVLATMGPGDIAFVPSPTYPIHTYSVIIAGADVRSISLTSGEGDFVERAERAI
                     KGVWPRPKMMILSFPHNPTTQVVDLDFFKRVVAFAKEHKMLVIHDLAYADLVFDGYKA
                     PSILQV"
BASE COUNT         1793 a         2979 c         3092 g         1785 t
ORIGIN      
        1 aaggattacg aagaccggcg caaggtggtt ccgattcggg aactcaaggc ggggatgacc
       61 gtacccgttc gcgggaagat cctttccgtg caggggggag gaaaggggtt ccgccccgcc
      121 caggtcctcg aggtcgtgat cgccgacggg acggggcgcc tctccgcgaa atggttccgc
      181 ttccatccat ccctcgccga gcggttccgc gtcggcgatt cggtgacgct ctgcggatcc
      241 gcgcggtggt tccgcttctt ccccgaaatg caccacccgg agatcctcgc ggaggaagat
      301 gcggtcgatc cggtccattc cggccggatc gtccccgtct acccggaggt ggaagggatt
      361 ccgccacggg tcctccgcaa gatccagtgg gaggtggtcc agcggcacgc ttcgctggag
      421 atcgaatgtt tcccatccag catcctcgcg cgggtcggga tcccacccct ccaggaatcg
      481 ctcgcttcca tccacttccc gcgcgacgac gccgacgcgg atcgcttccg ggaattctcc
      541 tctccccagc agcggcgcct gatcttcggc gaacttttcg ccctccagtg ggcactggcc
      601 ctccggcggg cgggaacgga gcaggaggag gcgatcccgc ttccgtggga ccgcgaaatc
      661 gtcgatgaga tcaagcgccg gcttccgttc gatttgaccg gcgcccagcg gcgcgtggtc
      721 aacgagatcc tgaaagacct cgggaagccg cacccgatgc accggctctt gcaaggggac
      781 gtgggcagcg gcaagacgat cgtcgcctgg atcgccgcga tggtcgcttg gcggcacggc
      841 gtgcaggccg cggtgatggc gcccacggag atcctggcgg agcagcatta ccggaggttc
      901 ctggaacggt cggagggact accggtccgt gtagcgctcc tatccgccgc acttcccacg
      961 aaggagcgcg aggcagtgcg aaaacagatc cgggagggag aggcggacat cgtcgtgggg
     1021 acgcatgccc tgatccagga gagcgtcgcg ttccgcaact tggcgctggg cgtcatcgac
     1081 gaacagcacc ggttcggggt gcttcagcgc gcctcccttc gcaggaaggg aaggatctcc
     1141 ccgcacctcc tcgtcatgac cgccacgccg ataccgcgga cgctcgcgat tacgctgtac
     1201 ggtgacctcg atgtctccat cctcgacgag atgccccgcg ggaggattcc tgtacgcacg
     1261 aaggtcgtcc cggaggacgg tcggcggaag gtcttcgagg agatccgcag ggagatcgcg
     1321 cagggaggaa gggtgtacgt cgtcctcccg ctggtggaag agtccgacaa ggtcgctctg
     1381 cgagatgcca cccgcaccgc cgaacggctc agggaagcgt tccccgacgt aggagtgggg
     1441 ctgctgcacg gccggatgaa ggcggcggag aaggaagaga tcatggggcg gttcaagggc
     1501 ggcgatttgc aactactcgt ctccaccacg gtcgtcgagg tcggggtgga cgtcccggag
     1561 gctacggtga tggtcgtcga gcactcggag cggttcggtc tctcgcaact tcaccagttg
     1621 cgcggaaggg tcgggcgcgg gagccgccca tcgtcctgct tcctgatgac gggacgggag
     1681 aagggggagg aagcggcggc gcgcttgtcg gtcatggagg aaaccgtcga cggtttccgg
     1741 atcgcggagg aagacctccg gatccggggg ccgggcgact tcgccggcgt gcgccaatcg
     1801 gggatccccg acctcgtatt cgccgacctc gtacgggacg cctcgatcct gcgattggct
     1861 cgagagatcg ttggggaact gcaccgcgac gatccgggcc tgtcgtcgcc tgaccaccta
     1921 cggatccggc ggttcctcga aatgaggacg acggcgtcgg gcggcggaga tcccgctcct
     1981 tcctcccgtg attaatactt aggtttatcg caatctcgta taggattatc tcgcttattc
     2041 cattatcggg aggcatgcgg aaacaggatg tccggtttcg ggttgagcgg atttaccttc
     2101 tgccggaatg ccgttgcgct ggggtaccct ctccgggaat cgatccaatc catcctgccg
     2161 atcgtcgacg aattcgttgt cgccctgggg ccctccgatg acgggaccgg cgagttgatc
     2221 gagagaatcg ggagtcccaa gatacggatc gtcgaaacgg cttgggacga gaatcaaatg
     2281 accgacgcga gggttctgtc gacgcaggca accttcgcac tctcctgctg ccgtttcccc
     2341 tgggcctttc acctccaggc ggacgaagtg atccacgagg cggacctccc cggtctcaag
     2401 gcattgatgc tgcagcacct tgaaaaccgg caggcgctgg ggttcatgtt tcgtttcatc
     2461 catttcgtcc tggattatcg gtcgatcaat ccatgggcct tccgccgcga agtacggatc
     2521 gtccgaaacg atggccaact ccgatctgcg ggggacagtt gcggtttcgc ccggaaatcc
     2581 gacggtctgt acctcgggaa gaagctattg ggaagagaag tcctctggac gggggcgcgg
     2641 gtcttccatt actcctcggt catgccgcga agcgcgttgg agaaaaaaat atcatccatg
     2701 agccgaatcc ggcacgggga tccgccggcg aagtccctgg agaaaattac ccgcgatggc
     2761 attgcgagcc tcgagcgaca atattccatc atgaaagagt accgggggag tcacccctcc
     2821 gtgatgcagg aaaggatcgc cgccgccgaa cggttccgac cgcgtgtcaa ccgctggttg
     2881 aactggcggt tctacttgaa tctcttcacc cacggattca aggggtgagg ccccatccga
     2941 tcacaagata ttggacaatc gcttcgctac cgtcttcttg gcttttttta catttttctt
     3001 tacgcctttc acgaactttg acgtcaactt ctccttgagt ttttttacct cggtttcctt
     3061 tttttccagt tttttgccta gggtcgtgag gtctttctcg accgaacgga cacgtttctt
     3121 gagttgacct tcgatgtttc cgagtttctt ccggatcgct ttcgtgccgt ccatggattc
     3181 ctccttctct tcaatggatg ttcctacttt atcattgccg ttcttcgttt tccaagcccc
     3241 ggatcgcttc ggcgatatcc tcgctcaatt tctccagggc gatgcagtga gcggagacga
     3301 gggcggcata accgacaccg ggtacgggtt cccgcaccaa ggtgcggccc gacctgaccg
     3361 caccgtcccc cgccctgcgg accacccaga cggcttccag tacggctgcg tcgccgggtg
     3421 tggagtcgaa gaccatcacg tctacggcgg cgcggtattt tgcggcggaa gacgtggaat
     3481 gcggaaagac ggacacctgg ggggcgccga ggagggcgga gaggtttccg gcgatccccc
     3541 gggcgatgtc gtcccggagc ggtgatcccc accggtggaa ctcgtcgatg aagacctggt
     3601 tggggcctgt ccgcacaacg atctgcggcc ggtcgacgat ctccggaacg gagaccggtc
     3661 cgacggcgac ggaatagccc gcagtcaccg gtgccgctcg cgccgagggg ctcagagtat
     3721 agaaatccgc ccgggaggag gcgcagcccg ccccggtcgc ggccagaatg caacataaga
     3781 cggtaaaggc tgtccggttt tgcatcaggg cttctcctgg gtcttgccgc gtatgagagc
     3841 gccggggttt ctctcgagat aatcggccag cgcgcggacc gcgccggcgg cgcgggagat
     3901 ttcccgcatg gcgtcccgca gatcctgctg cgccggagca tccggcccca ggaacgtgtt
     3961 gtccatgttc cccagcatgc gttcggcgga ggagaccgcc cgcctcagtt cctcgagggt
     4021 tttcttcgcc tccggaacga gttccccgtc gacgcgttcc agcgtgcggt tcgcgctttg
     4081 caacgtccgg tcgagcgtcc cgagcgtctt tttcgcttcg ggaagggttt ccccttcgag
     4141 gagtgccagc gtccgatcga gggccgcgat cgccttcctc gcatcgttgc cgatctcttc
     4201 gacgggcacc ttgtcgagtt tcacgagaag gctcttgaga ctcgcctgga gattcgccat
     4261 ctcgccaggc accaccggga acgcaggcgg tttccgattc cagtcgatca acgctttcgg
     4321 agcgtccgga aagtagtcga gggcgacgta catttgcccg ctgataaggc tcgcgctgcg
     4381 cagttgagcc cgcagcccct tttcaacgac ctgtcgctgc atgaactcaa gcctcccctt
     4441 tatcggagtg gggctctccg cggccgcccc ctgcattccc tgcggtacgg ggaaacggtg
     4501 ctggtaggtc acgatctcca cgcgcgtgtg gatgctttgc gacccgggga cgtaggcgag
     4561 aaagactccc gtgacttcgc cgaccggcaa gccgaggaaa tcgacggggg cgccgaccga
     4621 tagaccccgc agcgatccgt ggaaatacag cgcataccgt tcggcatcga cctcgtgggg
     4681 ggacagcgcg gtgacccggt cgtggaaaag ggggaagacc gcattatcca cgacgggatt
     4741 gttccccgtc ggagagggag gcgtctcgaa ggcgattccc ccgaccagca gggacagaag
     4801 cgactcggtc ttcacggcga gcccgctggt tcccaccgac acatcgatgc cgctctcttc
     4861 ccagaaacgg gcgttcgatg tcacgaaccg gtcgaagggg gcctccagga agatccggat
     4921 gtccaccgac ttcccgtttt ccaccaggcc gtaggagatc acctgcccca cgctcaggcg
     4981 ccggtaatac accggggagc cgatgcccag cgagcccagg gtatccgatc gcaacacgaa
     5041 ctcccttccc ggcaggccgg aagttgcggg gggagggact tcgagtccga cgaaggcgtg
     5101 tcctttctcc gtggactttc ccggctcgaa gcggatgtag ttcccggaga gcagggtacc
     5161 gatgccggag actccgctca aggtgacgcg cggcttcacg atccagaatc tggcgccttt
     5221 tacgagaagt ccttccgcgc tcttttccat tttcgccgtg atgaggatcc gggagtagtc
     5281 ctccgagagg tttacgcccg tcaccttgcc gatttcgatg tccttgtact tgatggaggt
     5341 tttaccggcc tcgacccctt cgccggaacg gaatgcgatc aggatcgtcg gcccctcgct
     5401 catgtactgc tggaaggcga ttcccacgcc gaccgccgcc gcgaggatcg gaatgatcca
     5461 gacgatggag agcctggcac gacttttggg tatgctctcg gcctgcggga cgttcgtgaa
     5521 atcggagttg tcagacatct tccccccgct tttccccgcc ggaatcccag atcagtcggg
     5581 ggtcgaaagt gtccgccgcc aaaatcgtca ggatgaccac tgcggtgaag aacaacactc
     5641 cgcttccggg aaccaccgac atgaacggcc acaattggac gagtgcgacg gagaacgtcg
     5701 cgacgaacac gtccagcatc gaccaccgcc cgatggcctt cagcatgcgg tacaggcgca
     5761 cccgctcgcg cctactccgg ctcgagcgaa gctgcaccgt gatcagcagg taggagaggg
     5821 cgaccagctt ccccaggggg atcatgacgc tggcgaccag cacgatcaga gccagcaccc
     5881 acgatccgtc cgcgtagaga cggacgactc cgccgatgat ggtggtcggc acgacggact
     5941 tgagcgtact cgtggtcatc accggaagaa cgttggccgg aaggtagcat atcgccgcgg
     6001 cgacgatcag cgcccaggtc cgctggatcg ggtcgcggtg ccggaggacc gtcggttctc
     6061 cgcaccgcgg gcagttcccg ggctcctccg gtccggccgg ctgggaaaga aggccgcacg
     6121 ccccgcacag gacgactccc gcctgcgcac ccgtcagcgg ggcgcggggc atcgacccgt
     6181 ctccggccat tcgccgtttt cccaccgcac ccgcggccag atctcccggg gttcgaagtt
     6241 ggcggacatc cctgcgagca ggaagacgag cgcgccgacc gcgaaaattc cgatgccggg
     6301 ctccaccgtg gcgagcgatg cgatcttgac cagcgagacc aggatgccga gcatcatgac
     6361 atcgaccatc gaccacacgc cggaccgctc cgcccagcgc agcccggcgc ccacccaacg
     6421 tggcgccgga ggccgccggg aggcaagcga tatcgccagc atgaacacga tgtcgagggc
     6481 tggcgcgacg acgacgaaga gcgcgacgag gacggcggtg accttttcgt cctgcagcca
     6541 catcgcctgg acgccgccca ggatcgtggt gctcgacgtc ctgccggaga tcgaaaccct
     6601 catcaggggc tccaggttgg cgaggagata ggcgatcaag gccgctaccg acagcgccat
     6661 cgagcggtcg agggaacccg gtttgttgga cacaagcgcc aagccgcagc gcgcacagca
     6721 ggcccttccg ccgggcggaa gggaaggcat gcgctgcagg aggtcgcagt cggggcaggc
     6781 aacgatgtca acgggcaaag gcgccatccg gtcgtttccc cttcagaaaa tcattgtatc
     6841 cgcgtccgcc tggaaattgg gagggggaaa ttcttctcct ccgcggtttg atatcacgag
     6901 cggagaacgt gatcctgcgg tcctgagagg acgggggcgg agggtgagat cgatgcgggg
     6961 cgctgtacgc ggaatctccc cttgctgccg cggcatcaaa atggaaaatg atccaggagg
     7021 gatatcccgt gaaaacgatc cttgcaactg cccttattct tgcgcttggg atcacgggga
     7081 tctccctgtc gggagcagtt cacgcgcaac gcatggccgt ttcctcgagc cagatcttcg
     7141 tccacggcac accggtgtgc gtgatccagc aggcagggga gatcattgcc ttggtcggaa
     7201 tatgcagtac atccgagggc gaatcgcggg aagaagacct cggaagcggg gggggcttcc
     7261 acggagagga accttccgac ccgcggatgg ggctccctcc ggggcaccca cctgtcgaat
     7321 tccccccgac cttgggggaa ggacgcagga tcctcatatg aggggtatcc atcttctacg
     7381 agcggaggga tggcgatggt gcggatagcg ctcgggaaga gcgatctcat ggtctccagg
     7441 atgggactgg gctgcatggg catgtcggag ttctacggtc cgggcgacga ggcggaatcg
     7501 gtccgcacga tccaccgggc ccttgaactg ggaatcaatt tcctggacac ggcggacatc
     7561 tacggaatgg gacgcaacga ggaactcgtc gggaaggcgc tccgcgaccg tcggaaccag
     7621 gcggtactgg cgacgaaatt cggcaatgtt cgcggaaagg acggaagctg gcagggcgtg
     7681 aacggcaaac ccgactacgt ccgctcatgc tgcgaagcca gcctgcggag cctcggcgtg
     7741 gacgtcatcg acctgtatta ccagcaccgg gtcgatccgg aaacgcccat agaggaaacg
     7801 gtcggggcca tggcggacct ggtgcggcag gggaaagtcc gctatctggg attgtccgaa
     7861 gcggcgcccg tgacaatccg cagggcctgc gccgttcacc cgatcacggc gttgcagacg
     7921 gagtattcgc tctggacccg tgatcccgag gccgaagtgc tcccaacctg ccgcgaaaac
     7981 ggcatcgcct ttgtcgccta cagcccgctg ggcaggggga tcttcagcgg tgcgatccgg
     8041 agcctggaca accttgccga aggggactac cggcgcaact ccccacgttt ttcgggagaa
     8101 aatctgtccc acaacctttc cctggtggac cgtctcacgg agatggccac cgggaagaag
     8161 tgccgtccgg cccagcttgc cctggcgtgg ttgttgtccc ggggacagga catctttccg
     8221 attccgggga ccaagcacgt aaaccgtctg gaggaaaacg tcggggcatt gcaggtgccg
     8281 ctgacgccgg aagaggtcac ccggctcgat acggcgtttc ccgcgggagc tgccgcgggg
     8341 aatcgttacc ccgagcaggc gatgaaggcg ctacaccggt aaccggaatg cggtaggcgg
     8401 gcttcataca gaagccgctg aattccgtcg agagcacggt agcctgaacc gattgccctc
     8461 cgatgccccc gctggttcgc tattctccca acttctcctt cacggagcgg atcttcgcgt
     8521 ccatcgaagc cgccttgtcg cggcggtcgt cgatcttgat cagggtggat acgcgaacgg
     8581 cgccctggct gaacgggttc tcgtgcatct cgcgcaggac cggcaagatc gcgtcgaggt
     8641 ccccctcgac aatcgatccc atcgcggtca gttcatgctt gagtccgctt ttccgaagga
     8701 tccgttcgac gccggcgacg tagcgggaaa ggcttgagct ggcggttccg aggggagcga
     8761 tcgtgacgaa tacgatggcc attccgttcc tcccgcgtat gcatgataga tggatggaaa
     8821 tctaccatgt ttgggaagag cggacaatca tcgcctggcg acgatcccgt gattgttcga
     8881 cggccgggtt cttaccacgc gtacgcatgg cgggcaaatt gacttggacg gcgcgattca
     8941 ttagcatgtt tgattacact tgacgggaga ttccccaatg gaagagttcg cgagaatcca
     9001 gcgtttgcct ccgtacgttt tcgcggtcgt agttgatctc aagtcgcgaa tgcgccacgc
     9061 cggcgaggat atcatcgacc tggggatggg aaaccccgat ctccccacac cgaagcacat
     9121 cgtcgacaag cttgtcgagg cggtccgcaa tccgaggaac caccgttatt cgctttcccg
     9181 cgggatcccg aagctgcggc tggcgatctg caactggtac gagcggaaat acaacgtgga
     9241 tctcgatccg gaaacggagg cgatcgccac aatcggagcg aaggaggggt tgtcccatct
     9301 cgtgctcgcc acgatgggcc cgggggacat cgcgttcgtt cccagcccga cgtacccgat
     9361 ccacacctac tcggtgatca tcgccggggc ggacgttcgg tcgatctccc tcacgagcgg
     9421 ggagggggac ttcgtcgagc gcgcagagcg ggcgatcaag ggggtgtggc cgcggccgaa
     9481 gatgatgatc ctctcctttc cccacaaccc gacgacccag gtggtggacc tcgatttctt
     9541 caagcgcgtc gtcgcgttcg ccaaagagca caagatgctg gtcatccacg atctcgccta
     9601 cgcggacctc gtcttcgacg ggtacaaggc gccctcgatc ctccaggtc
//
LOCUS       DASVJL010000122         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1238029, whole genome shotgun sequence.
ACCESSION   DASVJL010000122 DASVJL010000000
VERSION     DASVJL010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238029"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VF357_02395"
     CDS             <1..550
                     /locus_tag="VF357_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX7523024.1"
                     /translation="QLGMVEMVKEVFAGYKVKKVITQCPHCFNTFRNEYPQFGVDVEV
                     VHHSVLLRDLIAQGKLSPRKANNVALAFHDSCYLGRHNDIYDAPREALAAIPGLAINE
                     MRKNREDGFCCGAGGGGMWMESPGKRINYLRFGQAMATGANAVGSACPYCLMMFDDAI
                     KYNNLDDSVKAKDIAELVADSL"
     gene            703..>1701
                     /locus_tag="VF357_02400"
     CDS             703..>1701
                     /locus_tag="VF357_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX7523025.1"
                     /translation="MDSQTETILSTLLKKRLKTLGYPSLRKFHADRPGLGLSYEVLRQ
                     VVYGGHVPRPETLFRILGSMQFSSSQVRKICGMHYGDYLPISSAPTDASLPSPAQAGA
                     GTPESPSDPGASYPAERSRQENEPSSTPLEEPGEILSRLRASLQRIPVSGNEDFWEMV
                     EALARIAEQKVRRSAGRQAEQPLLFAGEPEAIYQFLVRKNRIPPFLSRGENLTFDFVG
                     GIDYRDRFRGALLGSAVGEALGAVTQGLTPRDVLELFGELDALPVVASARRAAAPHNS
                     LLLSVARSLLPDGVLDPERTADAIARAGRRDDPPGLSGFARNLLERGLPWHEAGENA"
BASE COUNT          304 a          568 c          531 g          298 t
ORIGIN      
        1 ccagttgggg atggtggaaa tggtgaagga ggtgttcgcc gggtacaagg tgaagaaggt
       61 catcacgcag tgcccccact gcttcaacac cttccggaac gagtaccccc agttcggggt
      121 ggacgtcgag gtcgtccacc attccgtcct gctccgcgac ctgatcgccc aggggaagct
      181 ctccccccgg aaggcgaaca acgtggcgct cgccttccac gactcgtgct atctcggccg
      241 gcacaacgac atctacgacg ccccccggga ggcccttgcc gccatcccgg gtctcgcgat
      301 caacgagatg aggaaaaacc gagaggatgg gttctgctgt ggcgccggcg gcggggggat
      361 gtggatggag tccccgggaa agcggatcaa ctaccttcgc ttcggtcagg cgatggccac
      421 gggcgcgaac gccgtcggtt cggcgtgccc ctactgcctc atgatgttcg acgacgcgat
      481 caagtacaac aatctcgacg attcggtgaa ggcgaaagac atcgcggaac tggtggcgga
      541 ttcgctgtag cagtaccttg aacagcacgg agccgggaat tgcaagggaa gttgccatcc
      601 ggcaacttcc cttttttctt ttccaacttc tcttgttgcc atacaatgat gcttgccgaa
      661 acagggcgta aaggaaatcg caagaaaggg aggacggcgg ccatggactc ccaaaccgag
      721 accatcctct cgactcttct gaagaaacgc ctgaaaacct tggggtatcc ctctctccgg
      781 aagttccacg ccgatcgtcc gggccttggc ctgagttacg aggtgttgcg gcaagtcgtc
      841 tacggcggcc atgtcccgag gccggaaacc ttgttccgga tccttggatc gatgcaattc
      901 tcctccagcc aggtccggaa gatctgcgga atgcactacg gggattacct cccgatctct
      961 tccgcaccca cggacgcttc cctcccgtcc cccgcgcagg caggtgccgg gaccccggaa
     1021 tctccgtcgg atccgggcgc atcgtaccct gccgaaagat cccggcagga aaacgaaccg
     1081 tcttccacgc cgctggagga accgggagag atcctctccc gcctccgcgc ctcccttcag
     1141 cgaatcccgg tttccggcaa cgaggatttc tgggagatgg tcgaggcgct ggcgcgaatc
     1201 gcggagcaga aggtccgccg gagcgccggt cgacaggcgg agcagccgct cctgttcgcc
     1261 ggggaacccg aggcgatcta ccagttcctc gtccggaaga accggatccc cccgtttctc
     1321 tcccgcggcg aaaacctcac gttcgatttc gtgggcggga tcgactaccg ggaccgcttc
     1381 cgtggcgccc tgctgggctc cgccgtcggc gaggccctcg gcgccgtcac gcaggggctg
     1441 acccccaggg acgttttgga actgttcggg gagctcgacg ccctgccggt cgtcgcgtcc
     1501 gcccgccggg ccgcggcgcc gcacaactct ctcctgttgt cggtcgccag gtccctcctc
     1561 ccggacggag tcctcgaccc ggagaggacg gccgacgcga tcgcccgcgc gggccgtcgg
     1621 gacgaccctc cgggcctctc ggggttcgcg cgcaacctcc tcgagcgcgg gctcccctgg
     1681 cacgaggcgg gggaaaacgc c
//
LOCUS       DASVJL010000123         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_300197, whole genome shotgun sequence.
ACCESSION   DASVJL010000123 DASVJL010000000
VERSION     DASVJL010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300197"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VF357_02405"
     CDS             complement(<1..864)
                     /locus_tag="VF357_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HEX7523026.1"
                     /translation="MKVLILGVNGFIGHHLTARILKETDWKIYGMDLSAERLGKSASH
                     PRFDFVEGDISINKEWIEYHIKKADVILPLVAIATPKVYVERPLSVFELDFEENLRIV
                     RQVHRYGRRIVFPSTSEVYGMCGDKEFDEENSPLVLGPIAKERWIYSCCKQMLDRVIW
                     AYGRQGLAFTLFRPFNWIGPRLDSIETAKEGSSRVVTQFIADLYLGKPIQVVDGGEQR
                     RCFTYVDDGIAGLMKILANRNGCANGGIFNIGNPANDHSIRELAEMLRKLYAKHPRNR
                     GKKVPPIIEVNS"
     gene            complement(861..1835)
                     /locus_tag="VF357_02410"
     CDS             complement(861..1835)
                     /locus_tag="VF357_02410"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HEX7523027.1"
                     /translation="MRAVVFAYHNMGIAGIRALLDHGFAIPMVLTHEDDPRENRWFGS
                     VAEFCRERGIPVFFPKDVNAPPWPDRIRTASPDLLFSFYYRSMLKKEILEIPPLGAMN
                     LHGSLLPKYRGRAPVNWVLVKGETETGVTLHFMTEKPDAGDIVGQAAVPIAFGDTALT
                     LFGKMEDAASRLLADLLPRIAIGEIPRRPNDLSRGSYFGGRTPEDGRIDWSRPAMEIY
                     NLVRAVTRPYPGAFTELGREKLTVWWAIPLPAESGSVPSPGTIRVAGGLSYAMAGGCA
                     APVSGLRRIVVETGDGWLQLEEIEWGTRTAKGEGIVDLLAGAANGRLS"
     gene            complement(1841..>2240)
                     /locus_tag="VF357_02415"
     CDS             complement(1841..>2240)
                     /locus_tag="VF357_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523028.1"
                     /translation="PALATLFAKRMTEIPVGHAERHAGVSNYNLWRLMNLQFDLLTSF
                     TEFPLRVLLYMGTGMAFLGVSFGVLLVVMRIFYGAAWAANGIFTLFAVLFFFVGALFF
                     ALGIMGQYIGRIYHEVRKRPRFTIRKLHES"
BASE COUNT          391 a          776 c          698 g          375 t
ORIGIN      
        1 cgagttgacc tcgatgatgg gggggacctt cttcccgcgg ttccgggggt gcttcgcgta
       61 cagcttccgg agcatctcgg cgagctcccg gatcgagtgg tcgttcgccg ggttcccgat
      121 gttgaagatc ccgccgttcg cacagccgtt ccggttcgcg aggatcttca tcagccccgc
      181 gatcccgtcg tccacgtagg tgaagcagcg gcgctgctct ccgccgtcca ccacctggat
      241 cggcttgccg aggtacaggt cggcgatgaa ctgcgtgacc acgcgggagg acccctcctt
      301 tgcggtctcg atcgagtcga gccgcggccc gatccagttg aaggggcgga agagggtgaa
      361 agcgagccct tgccgcccgt acgcccagat cacccggtcc agcatctgct tgcagcacga
      421 gtagatccac cgctccttgg cgatcggccc gaggaccagc ggcgagttct cctcgtcgaa
      481 ctccttgtcg ccgcacatcc cgtacacctc ggaagtcgag gggaagacga tccgcctgcc
      541 gtaccggtgc acttgccgga cgatgcgcag gttctcctcg aagtcgagct cgaaaaccga
      601 cagcggccgc tcgacgtaga ccttcggcgt ggcgatcgcc accagcggca ggatcacgtc
      661 ggccttcttt atgtggtact cgatccactc cttgttgatc gagatgtcac cctcgacgaa
      721 gtcgaaccgg ggatggctcg ccgattttcc cagccgctcg gcggaaaggt ccatcccgta
      781 gatcttccag tcggtctcct tcaggatcct cgccgtcagg tgatggccga tgaacccgtt
      841 cactcccagg atcagcacct tcatgaaagc ctcccgttcg cggcgccggc gagaaggtcg
      901 acgatcccct cccccttcgc cgtccgcgtc ccccattcga tttcctccaa ttggagccac
      961 ccgtcccccg tctcgacgac gatccgccgc aggccggaaa ccggagcggc gcatcccccc
     1021 gccattgcgt acgacagccc cccggcaacc cggatcgtcc ccggagacgg cacgctaccc
     1081 gactccgccg gcagggggat cgcccaccac accgtgagtt tttccctccc gagttccgtg
     1141 aacgcccccg ggtagggacg ggtgaccgcg cgaacgaggt tgtagatctc catcgccggt
     1201 cgggaccagt cgatccgccc gtcctcgggc gtgcgcccgc cgaagtagct cccgcgggag
     1261 aggtcgttcg ggcgccgcgg gatctccccg atcgcgatcc ggggcagcag gtcggcgagg
     1321 agcctcgaag cggcatcctc catcttcccg aacagcgtga gcgccgtgtc cccgaaggcg
     1381 atcggcaccg ccgcctggcc cacgatatcg cccgcgtcgg gcttctccgt catgaagtgg
     1441 agggtcaccc cggtttcggt ctcccccttc acgagcaccc agttgaccgg cgcccgtcca
     1501 cggtacttcg gcagcagcga accgtggagg ttcatcgccc caagcggcgg gatctcgagg
     1561 atctcctttt tcaacatcga ccggtagtag aaggagaaga gcaggtcggg cgaagccgta
     1621 cggatccggt ccggccacgg cggcgcgttg acgtccttcg ggaaaaaaac cgggatcccc
     1681 cgctcccggc agaactccgc cacggaaccg aaccaccggt tctccctcgg atcgtcctcg
     1741 tgggtcagga ccatcgggat cgcgaagccg tggtcgagga gggcgcgaat ccctgcgatc
     1801 cccatgttgt ggtacgcgaa gacgaccgcc cgcatcccgc tcagctttcg tggagcttgc
     1861 ggatcgtgaa gcgcgggcgc ttgcggacct cgtggtagat gcggccgatg tactgcccca
     1921 tgatcccgag ggcgaagaag agggccccca cgaagaagaa caggacggcg aacagggtga
     1981 agatgccgtt cgccgcccac gcggccccgt agaagatccg catcacgacc agcagcacgc
     2041 cgaaggagac accgagaaac gccatccccg tgcccatgta gaggagcacg cgcaggggaa
     2101 actccgtgaa cgaggtgagc aggtcgaact ggaggttcat gagcctccac aggttgtagt
     2161 tggacacccc cgcgtgccgc tccgcgtgcc cgaccgggat ctccgtcatc cgcttcgcga
     2221 agagggtcgc caaggcgggg
//
LOCUS       DASVJL010000124         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_487996, whole genome shotgun sequence.
ACCESSION   DASVJL010000124 DASVJL010000000
VERSION     DASVJL010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487996"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="VF357_02420"
     CDS             complement(<1..1328)
                     /locus_tag="VF357_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7523029.1"
                     /translation="MRIGKKILAPGGILFLLLATVFPMKAVAEEVPFPVSRYVESALV
                     DNPSLDSMRERIRMKENAAIKAGALDDPKLRMAILNVPTRSWSFREEDMTGKEITLSQ
                     MFPWPGMLKTRTHMALREKEETEFVLEEMRSMLRSEIKMTYAELASVRKQIEAVHRSQ
                     DVLKEIVGVTQEIYAVGKGSQPDVLRGQVEFGRMREMRIGLENREQVLSVRLNTLAAL
                     PADRPVPPLEDLPEISLPYGQADLVGIYEAERPARKALQARVHRGESSVRMAELAGKP
                     EFEVSFSYMQRDAMPDGTKRSDMVSSMVMMTLPIWRKEKIEPGIREMEAEKRMAGREL
                     ANLDLETSNSIGRSLATMKSRREAAVLYRTTLIPQAEQSFQATAGSYRVGKTDFPMLM
                     DAALAILSFRREYAAMAGDLHMEKARLEAAVGRELDGGTPAPGEMAPPNT"
     gene            complement(1467..1751)
                     /locus_tag="VF357_02425"
     CDS             complement(1467..1751)
                     /locus_tag="VF357_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523030.1"
                     /translation="MEAETGMQGGTGMRDSADTIANEDPPVVEGQRGPTWRGWVLSIL
                     VAVILSVTATLLLGGSGAFRSNRAVAAGSAGSGCGSIAGSDCCPPADAGK"
     gene            complement(1785..>2283)
                     /locus_tag="VF357_02430"
     CDS             complement(1785..>2283)
                     /locus_tag="VF357_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7523031.1"
                     /translation="SALLPDGLADFRRGTIGFVFQAYHLLPYLTARENVMVPMSPERV
                     SPREKAERADALLAAVGLAGKEDRLPSQLSGGESQRVAIARALVHDPPVLLADEPTGN
                     LDSATEEQILDLFSGWHGRGKTVLMVSHNKANLARATRSIRLRDGRVEEDRLLRSFPE
                     ELVAV"
BASE COUNT          374 a          823 c          660 g          426 t
ORIGIN      
        1 ttcgtgttcg gcggcgccat ctctccggga gccggagtcc ctccatcaag ttccctgccc
       61 accgccgcct cgaggcgcgc tttctccata tgcagatccc ctgccatcgc cgcgtactcc
      121 cgtcggaagg agaggatcgc cagggctgcg tccatcagca tcggaaaatc ggtcttcccc
      181 acgcgatacg atccggcggt cgcctggaac gactgctccg cctgcgggat gagcgtggtc
      241 cggtagagga ccgccgcctc acgccggctc ttcatcgtcg cgagcgatcg cccgatcgaa
      301 ttggaggttt cgaggtccag attggcgagc tcccgaccgg ccatcctctt ttcggcctcc
      361 atctcccgga tccccggctc gattttttcc ttccgccaga tcgggagcgt catcatcacc
      421 atcgaggaaa ccatgtcgga gcgcttcgtc ccgtcgggca tggcgtcccg ctgcatgtag
      481 gagaaggaga cctcgaactc gggcttgccg gcgagttccg ccatccgtac cgaggattcc
      541 cctctgtgga cccgggcctg tagagccttg cgggcaggac gctccgcctc gtagatcccc
      601 acaagatcgg cctgtccgta ggggagcgag atctccggaa ggtcctccag cgggggaacc
      661 ggccggtcgg ccggcagggc ggccagcgtg ttgaggcgca ccgagaggac ctgttccctg
      721 ttttcgaggc cgatccgcat ctcccgcatc cggccgaatt ccacctggcc ccgcaggacg
      781 tcgggctggc tccccttgcc gacggcgtag atctcctgcg tgacgcccac gatttccttg
      841 aggacgtcct gggacctgtg cacggcctcg atctgcttac gaaccgaggc caactccgcg
      901 taggtcatct tgatctcgga ccggagcatg ctgcgcatct cttcgaggac gaattccgtc
      961 tcttccttct cccggagcgc catatgcgtc cgggtcttga gcatccccgg ccaggggaac
     1021 atctgggaga gagtgatctc cttccccgtc atgtcctctt cccggaaact ccaactccgg
     1081 gtcgggacgt tcaaaatcgc cattcgcagc ttcgggtcgt cgagcgcccc ggccttgatc
     1141 gcggcgttct ccttcatccg gatccgctcc cgcatggagt ccagggaagg attgtcgacg
     1201 agtgccgatt ccacgtagcg cgacacgggg aagggaacct cttccgcgac cgctttcatg
     1261 ggaaatacgg tcgccaggag gaggaagaga atcccccccg gcgccaagat tttcttgccg
     1321 atccgcatcg ctcgtgcctc catcccgtga tcagcctttc cgcaatccgc ccgggttttt
     1381 cgcttcaccc gtattatcgg actgtggcga acaataaaat ttataaatta agcggttgat
     1441 aaggattcgc gctaaccccc tgccgttcac ttccccgcat ccgccggcgg gcaacagtcg
     1501 cttccggcga tggacccaca tccgctcccg gccgatcccg ccgccaccgc gcgattcgac
     1561 cggaacgcgc cggaaccgcc cagaagcagc gtcgccgtca ccgacaggat gaccgccacg
     1621 agaatggaga gcacccatcc gcgccaggtc gggccgcgct gcccttccac gacgggcgga
     1681 tcctcatttg cgatcgtatc cgccgagtcc cgcattcccg ttcccccctg cattcccgtc
     1741 tccgcttcca tcgccccgtc ctttcttctt ttgaagacct gcgttcagac cgccaccaac
     1801 tcctcgggaa aggaccgcaa caaccgatcc tcctcgaccc gcccgtcgcg cagtcggatg
     1861 gaccgcgtgg cgcgcgcgag gttcgccttg ttgtgcgaca ccatcaggac cgtcttcccc
     1921 cgaccgtgcc acccggagaa gaggtcaagg atctgctctt ccgtcgcgct gtcgaggttc
     1981 cccgtcggct cgtccgccag caacacgggg ggatcgtgga ccagcgcccg cgcgatcgcc
     2041 acccgctggc tctccccgcc cgacagctgc gaaggaaggc ggtcctcctt cccggccagc
     2101 cccaccgcag caagcagggc gtcggcccgt tcagccttct cccggggcga gacccgctcc
     2161 ggggacatgg gcaccatcac gttttcccgc gccgtcagat acgggaggag gtggtacgcc
     2221 tggaagacga acccgatcgt cccccgccgg aagtcggcca aaccgtccgg caggagcgcg
     2281 gag
//
LOCUS       DASVJL010000125        17518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_206558, whole genome shotgun sequence.
ACCESSION   DASVJL010000125 DASVJL010000000
VERSION     DASVJL010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 17518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17518
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206558"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VF357_02435"
     CDS             complement(<1..937)
                     /locus_tag="VF357_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7523032.1"
                     /translation="MNVPVFTGLQKLEFQERPRPVADPGEVLVDVALCGICGSDVHGY
                     LNGIMIPVGTVMGHEAVGTVAETGKGVTGWRAGDRVAIKPIPECGECPYCRMGQFSLC
                     IEAFPRAIGISPANDGAFARTVEVKYPKAMLFRLPDEVTFEQAAVIEPLATSLHGVRM
                     SRFRPGDTAVVIGAGTIGLGVLQFLMLGGAGKVIVLEVSEKKREIARGLGADVVLDPL
                     KEGEKLRETVFGHTHGIGADLVYECAGVPQALQASYSLVKSGGQVLLIGINDKEVSIQ
                     PFFLSLWEVELKGILGYYDEFDHVIEFLRRKRINTE"
     gene            complement(964..1767)
                     /locus_tag="VF357_02440"
     CDS             complement(964..1767)
                     /locus_tag="VF357_02440"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7523033.1"
                     /translation="MGFLKGQAAIVTGGARGLGRGIALALAQEGASVMIADLLADKGK
                     ATAAEVAALGVGSAFLPVDVTKRDQVEGMVAETVKRFGRLDILVNNAGNTTHPAWCWE
                     MSDQDWQSVINTHINGTFYGIKAAAKVMVGRGYGRIVNLSSIAAVHGASSQINYSAAK
                     FAIKGMTYTAAKELGPHGITVNALQPGVIRSDMTAILLNPGEEKTALGTPVRRVGEPE
                     DVANAVKFFVNPASGFITGVVMRVDGGIATKLAAEDDLEAYARVYPGKE"
     gene            complement(1832..2317)
                     /locus_tag="VF357_02445"
     CDS             complement(1832..2317)
                     /locus_tag="VF357_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7523034.1"
                     /translation="MTMDAAQLAGALNVGTIAQVGFVVRNLEKSIREYERVMGIGPFM
                     MVDFRPEKSFVKGRASAIHLKIGIAQLTPALSVELIEVVSGQPYHKDFLEAHGEGVQH
                     LGYLCDDYDGVLAKAKSMGIDVLMWAETDVAGMGHVRGAYLDTLALLGTLIEVIEVKP
                     F"
     gene            complement(2320..3789)
                     /locus_tag="VF357_02450"
     CDS             complement(2320..3789)
                     /locus_tag="VF357_02450"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HEX7523035.1"
                     /translation="MRITKQLGLSKWGLLFVLVVLITSAVTLSAAEYPDPKTYLPTLS
                     AVDKHKAPYDDPRPYLTTFGPKQVLPKDFYAKLSFDVDSMKSLWAELVGFKAPDIVGK
                     IAPEIKPGKYTYKDLAKNPGLKALMWADLYNRIKPGAPPHGGNIPEFEIIPTRQLYWA
                     LPIAEATKKNLGKTKLDAKGYLLGETWAGGVPFPKPSGPFKAQQIMYNIEKRMLAWEL
                     NFYIISHVIGVNSSLNVDFDGGYDVKHVRLAGRTLMEPYGWFDKRAKERNEFKTFMFG
                     FQAPRDVAGVYQQALYSNDQNTADQLMLYIPSMRRVRKMSATDTQDPVMGQDQIYDDN
                     EGWMQKLSATRYPYKYEVLEEREYLVPAATADGAEYISSKGKEFRNVRMERRPLYVVK
                     LTQQDPNYVYGHRIFYVDAETFNFLHIENYNRKGKLYRTWDGNYGWFPEMGMFSWCGS
                     LILMRDHIDLHSGVQQPYQLPAAWGREDMSMKSLSSQGK"
     gene            complement(3826..5376)
                     /locus_tag="VF357_02455"
     CDS             complement(3826..5376)
                     /locus_tag="VF357_02455"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HEX7523036.1"
                     /translation="MEKGRFRWIGSVIVLALLGSQTPSGATEFNLLDKPATLMGYVDQ
                     GVSTSFRNGKSFDNKDGMNSAVFMSLLEGEYKPAPNVKVFMSGKFTADLAYPILSSNS
                     EWKDKEFNKSRDALFVDTGWDKMLNEAYVTWSPGNFFLRVGKQIVGWGETDGFRLMDQ
                     INPVDQRRGLGDVEFENTIIPIWLVRANYYVQPQSSWLQDIGLETVFNPNARFQADRP
                     IDLGNDVAGVWAPDVQVAPGVQLGSSRYTNKAPQDWNRSGMEFGARVKGVVNDMIFSL
                     NYFNGLSNSGQLRSLSGPDITLAPDGTPIAHLHVEERFPRYQMVGATFTSDFPAVSSS
                     ALGGVAPVLRLEGEYVMNNTLSTAGGVVGLPFGSYVKKDEIRYVVGLDWKVKADFLNP
                     KAFFFISGQFYHRHILDFDKDMAPAEVSAGMDLKDFLANIKKDNYVTTLLINTTYFHN
                     KLTPQIFWMRDMTLNGNMFKPQLTYDYSDKWHYTIGALFLEGKVPQKGFEPLKNKDQL
                     FATVKYRF"
     gene            complement(5440..7851)
                     /locus_tag="VF357_02460"
     CDS             complement(5440..7851)
                     /locus_tag="VF357_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX7523037.1"
                     /translation="METKSRFERFCWYLAQHQIKILVLVLAITAVFGVGISRIHTEVI
                     LSEMFPYDHPYLKLMGRFGQVFGGGGSGVVVAVKVKKGDIFNEKTLTKIKNMTSEVEL
                     WDEVYRVLTVSMASNSTKVVNAKGKGEITIESLMFPDVPKTPEAMELLKKNVFSNPAY
                     NGNLVSKDGTACLLVTECKENISYSRIFTLFRGLQQKYSDDNTSIHMVGFPILMGWIY
                     SLTPQMYMVFAFSILAIALVLWLIFDNFPGMISPLANAGILTIWGLGFIGFTGINFSP
                     LLYVLAFLVAARMIGNSHQIAYRYFEELDASKGDRKKASYETMRTMWVPNFAAVAADV
                     AGFAVLFIAKIVLMVHLAIIMSFWMATILFTGLLVPLVCSLVPQEVKSTEWAKESCQL
                     DRKAKFLMRITHFSIGPNTRYITGALILLLTIFCVWETSKLKIGDPTSGSPVFFSNHP
                     YNKDQAVINNTFDASSENFVLYYEGAAGSVYEPVVLNTFESFSRHMAEHLPDIYKSST
                     SILNIVSILNRTFHDGDMLWYQLPLEKEMLTGILGYIRNTVDRGTLGRFMDPTLERAQ
                     ITLYFADHTSDNLLRIRNAAYDFFRTNSMKIGHGEFHLAGGRIGMEIAVNEEMKRSHL
                     LIDLAVYLGILVLCSLCYRSIVAGLMLTIPLLLANSMAATYMSLRDIGLSINTLPIAA
                     IGAGLGVDFAIYLYSRAIEEFPLQHGDWTATIMQSVCTCGKAVVYTGITVILPILTWY
                     IFSDMKFQAEVGFFLSIIMAANVLLALTLHPLMIYIIKPKFISRNFEAEAATAPSASF
                     GLLQE"
     gene            complement(7892..8866)
                     /locus_tag="VF357_02465"
     CDS             complement(7892..8866)
                     /locus_tag="VF357_02465"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX7523038.1"
                     /translation="MSIVFILAALICVPLVSPAFVGQRMTDDLFSVNFGSEKHGWAVG
                     RWGSVLHTADGGKTWEKQGTGVNYTLTSVSFVDEKNGWAVGDQGTIIHTSDGGKTWEK
                     QKSPVTYFLMGVSFATKNQGWIVTERTNILHTEDGGKTWAVQFKDQDFILKSVSFCDE
                     RNGWAVGEYGFVYHTSDGGGTWTHQAGEFGFSDETGEVVGGSYLFDIVAVNPMTAWVV
                     GIDGYVAKTVDAGKTWRKQMTGVPKTHIFGVSAGEKNVLIAGDAVLLRTADGGETWKE
                     VKVEPSIRYGWLYRIGKRGKDGFVAVGQAGWIYGADAGAGEWKRVGTR"
     gene            complement(9163..10278)
                     /locus_tag="VF357_02470"
     CDS             complement(9163..10278)
                     /locus_tag="VF357_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7523039.1"
                     /translation="MLKSQDMLDVIKIVRTALECDQIDDLRKEVLKPLVEAFHVERCN
                     FFLFRQYTSPRLDLGNVITQGIDQKYLDLFRQHYYKMDPFLKAISASETVMTTSSIIS
                     NKDMIRSEYFNDFLKPQCIYKQMVILLKSRGQLCGTLALFRSEHEQDFSRFEKAKGEL
                     ISSSLGGALEKSIFIEKITKSEKIMSSFCMDLPYKGILILDQSIDPVYINEEAMETIS
                     YFVGKNEPSEKSAFALPMELYVQCRQFMESLKLNGETATSREVKLLNASSGKTISANF
                     RMISYFGDSKMCLICMNKNESGVLLSKQLDKLRITRREQEVIDLICEGLKNSEIGEKL
                     FISEYTVENHLRSIYEKMNVKNRINLLRKVMLYTGQR"
     gene            complement(10673..11818)
                     /locus_tag="VF357_02475"
     CDS             complement(10673..11818)
                     /locus_tag="VF357_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX7523040.1"
                     /translation="MGAACVAGVGLIPFGKHVERTLVDMGSEAAYLALEDAGIEPGRV
                     GFGVFASAAAPRLLGEMTSGQNIFWEVGINRIPILNVENACTSGSTAFVLACNAIAAG
                     QVDVALVVGAEKLCIPGVGLLNSGETELDTRLGMVTPAGFAMRAARYIHKFGASAAQL
                     ASVAVKNRGHAVLNPYAHFRKAVTLEEVLSSPVVADPLTRMQCCPMSDGAAAVVLCSQ
                     EEARRRRRAVEVKAAILCTGSYDNPADLDRWETDYRGCAKGYEAAGIGPEDVHVVECH
                     DAFTIAEILHYEALGLCGPGEGANLAVRGETRLGGRIPVNVSGGLIGRGHPPGATGLA
                     QVHEIVTQLRGEAGDRQVRNAKVGLAHCMGGDKDGDTKSFTMIVLAC"
     gene            complement(11818..12279)
                     /locus_tag="VF357_02480"
     CDS             complement(11818..12279)
                     /locus_tag="VF357_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX7523041.1"
                     /translation="MTRQEEPNIEEAVLPPVVMPGILDIPADGGQPRLLGGSCPSCGV
                     RYFPRPSRCRRCLGPVEASTVGAEGTVYSFTVIRIRPPLGLPEPYAIGYVDLKESGLR
                     VFSLLDPQAVEKFRIGIPVRLAVGALGNDARGASCLRPYFTPLVPEPGGVA"
     gene            complement(12331..13929)
                     /locus_tag="VF357_02485"
     CDS             complement(12331..13929)
                     /locus_tag="VF357_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7523042.1"
                     /translation="MGSADRVHARTIPHVLREKVKQFGNRTFFYFREQEFGYRDFDRA
                     SDQVAAGLQSLGIGKGDKVAILMGNRPEFLFLWFGLAKLGAVEVPINTAHRGDILAYM
                     VNKADCRMVVAESACLDPVVRVLKDLPGVERVVVLGQPGEALSALDRPATDYRSVTEN
                     DGRYDAPELLWSDPFAIMFTSGTTGPSKGPVLPQNYALLMGETISRSARYTGEDCLYN
                     ALPLFHGNAQVLSTMPALMSGARMVLAERFSASSFWSEVRRYGCTEFNYIGGILHILL
                     KAEPRQDDVDNPIRVMLGAGCTKDLFETVEKRFDLKILEGYGMSEIGLPLMNTIDDRK
                     PGSIGRPAFGCQVRLVDDADAEAGPGVVGEILVRPSQPYGMLLEYYKMPEQTVEAWRD
                     LWFHTGDYAVKDEEDFHYFVDRKKDALRRRGENISSYEVEKVVNAHAAVLESAAIAVK
                     DGMGEDEVMIVVTPRPGIPLRPEELFGHCRDRMAYFMVPRYVRIMERMPKTSTEKVQK
                     SELRKQGITPDTWDRVKAGLELKR"
     gene            complement(13960..16008)
                     /locus_tag="VF357_02490"
     CDS             complement(13960..16008)
                     /locus_tag="VF357_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX7523043.1"
                     /translation="MSSLAYGRDYEVVQRLRPEPMTAEEAAGQEKIEGIDFEIFRHKM
                     NMIAQEGKETTMKLGASTGMRWGDVAFAIYTAQGDMAVVATGIWFHAVLGQIPVKYIL
                     KHFVNDPSVKVDEGDSFFWNDPFYGGVHGADMGLAVPVFHQGKLVCFVGAVVHTGENG
                     GTDPGGLSNTARSRYDDGLLCPPIKVGERYALREDILTMIGSHVRDPRTMILDIKARL
                     AASRIAERRIQEVLGDKGQEFFIGSLRRILSVTAEAAKKKIALLSDGVYRQPRFLDTV
                     GTESTLVKINLTITKKGHGFFFDFEGSSPMIPDKPLNTYFHGILGLAMVYFCGWFFHD
                     LPANNGLLETIEWHFPEDSLINAKGEVSTSLAPFLQTAFSQGMFLCGARMSYSLDPLR
                     AGAAWYTGFGVPMFGGINQWGEPMADIFAEINSTGCGARPDMDGVDCAGSFFATMSDC
                     GDVETTEEDRPFLYLFRNYFRGSYGHGKYRGGAGSGYCIMPFHVPGVGLGAFGYGSRF
                     PATVGIFGGYAVPPPFVQTVRGSNMKDLLAQGSPSVPVNVEQALQDNGAETGRRELHD
                     SSMRVQPFQNGDTLYLPVGGGAGYGDALERDPEAVVADLRDGMVTPWAARNIYRVAYD
                     DETLRLDREKTRELREEARARRKQRGKPYGEFEKEWLKLRPKDEVIKYYGTYPVSGAI
                     "
     gene            complement(16005..>17518)
                     /locus_tag="VF357_02495"
     CDS             complement(16005..>17518)
                     /locus_tag="VF357_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX7523044.1"
                     /translation="TTAIVNAYIHGIMGEQLGHLVNELRDGGYKRPLLLVHNTGGMKK
                     ASRTRAVLTHNAGPVAGLHGSLTDGARYGQQNVIFTDMGGTSFDIGVITGGRLSTYDF
                     IPVIDRWRTNIPAIEVKSIGAGGGSIAWVNELLGGSLEVGPQSAGSMPGPACYDKGGQ
                     DPTVTDADLVLGYLNPDNYLGGKMLLDPDLSAQVIEKKIAKPLGIGVVEAALRIRRIV
                     DARMGQEVFSEVVVKGLDPRRFDLYACGGAGPTHACGFAPYVGVKRVITSPFSPVFGA
                     FGAYTLPVRQVWEKSKAMKIFRWADQAYSADAEGFNAVVCELKDLALRDFRLEGFSED
                     QVEFRLEIEMRYGMQYNLTKVVSPYLAIHGPNEFKEICDRFTEQYSATYSPEATFPRG
                     GINIECFYLTASVQNRLHKPEALPLRGLAPPDAARADSRKAHWSGGGPSETPVFVFEA
                     LEAGNRLTGPALVEARDTTYVIEPGWRFTLDPYRNGILEPVLAAVRAEEEKKR"
BASE COUNT         3298 a         5632 c         4946 g         3642 t
ORIGIN      
        1 cctccgtgtt gatccgtttt cgcctcaaaa actcgatcac gtggtcgaat tcgtcgtaat
       61 acccgagaat gcccttgagc tccacctccc aaagggagag gaagaacggc tgaatgctca
      121 cctccttgtc gttgattccg atcagcagca cctggccacc gctcttcacc agggagtagc
      181 ttgcctggag cgcctggggt acccccgcac attcgtacac gagatcggcg ccgatcccat
      241 gggtgtgtcc gaagaccgtt tcccgcagct tctccccctc tttcagaggg tccaggacca
      301 cgtcggcacc caacccacgc gcgatctccc tcttcttctc cgagacctcg aggacgatca
      361 ccttcccggc cccccccagc atgaggaact ggagcacacc gagcccgatg gtcccggcgc
      421 cgatcaccac ggccgtatcc ccgggccgga accgggacat ccggacaccg tgcaacgagg
      481 tggcgagggg ctcgatgacc gccgcctgct cgaaggtcac ctcgtccggc aaccggaaga
      541 gcatcgcctt cggatacttc acctccacgg ttcgggcgaa ggccccgtcg ttggcggggg
      601 agatcccgat ggcgcggggg aacgcttcaa tgcacaggga gaattgcccc atccggcaat
      661 aggggcactc cccgcattcg gggatgggct tgatggccac ccggtccccc gcccgccatc
      721 ccgtcacccc tttccccgtc tccgccacgg tccccacggc ttcgtggccc atcaccgttc
      781 cgacggggat catgatcccg ttcaggtacc cgtggacgtc cgaaccgcag atcccgcaca
      841 gggccacgtc gaccagcacc tcgccgggat ccgcgacagg ccttggccgc tcctggaact
      901 ccagcttctg caaacccgtg aacaccggaa cgttcatgga atctcctcta tgaatcgtct
      961 tggtcactcc ttgcccggat agactctcgc gtaggcttcg aggtcgtcct cggccgccag
     1021 cttggtcgcg attcccccgt cgacccgcat cacgactccc gtgatgaatc cggacgccgg
     1081 attgacgaaa aacttcaccg cgttcgccac gtcctcgggt tccccgactc tccggaccgg
     1141 cgtacccagg gcggtctttt cctcccccgg attgagcagg atcgccgtca tgtccgagcg
     1201 gatcaccccg ggctgcaatg cgttcacggt aattccgtgg ggacccagtt ccttcgccgc
     1261 cgtgtacgtc atccccttga tcgcgaactt cgccgcggag tagttgatct gggaggaggc
     1321 gccgtggacc gccgcgatcg acgacaggtt cacgatccgg ccgtacccgc gcccgaccat
     1381 caccttcgcg gcggccttga tgccgtaaaa cgtcccgttg atatgcgtgt tgatcaccga
     1441 ttgccagtcc tggtcgctca tctcccagca ccaggcgggg tgcgtcgtgt tccccgcgtt
     1501 gttgacgagg atgtcgagac ggccgaaccg cttcaccgtc tcggccacca tcccttccac
     1561 ctgatccctc ttcgtgacgt ccaccggaag gaaagcgctt cccactccaa gcgccgccac
     1621 ttccgccgcg gttgccttcc ccttgtcggc cagaaggtcc gcgatcatca cgcttgcccc
     1681 ctcctgcgcc agggcaaggg cgatgcctcg cccgagcccc cttgcgccgc cggtcacgat
     1741 ggcggcctga cccttcaaaa atcccatcga atcctcccat cagaaaatgt tgtccccgcg
     1801 cccggctctt cgaccgggcg cgggggttcc ttcagaatgg tttgacttcg atcacctcga
     1861 tcagcgtccc gagaagagcg agcgtgtcga ggtaggcgcc gcgaacgtgg cccatgccgg
     1921 ccacgtccgt ttccgcccac atgagcacgt cgatccccat gctcttggcc ttcgccagca
     1981 ccccgtcgta gtcgtcgcac aggtacccga gatgctggac cccttctcca tgggcctcca
     2041 gaaaatcttt gtgatacggc tgtccgctca ccacctcgat cagctccacc gacaacgccg
     2101 gcgtgagctg ggcgatgccg atcttcaggt ggatcgcgga agcccgtcct tttacgaagc
     2161 ttttttccgg tcggaaatcg accatcatga aaggcccgat gcccatgacc cgttcgtact
     2221 cccgaatcga tttttcgagg ttgcgaacga cgaagccgac ctgggcgatc gtgcctacgt
     2281 tcaatgcccc tgcaagctgc gctgcgtcca tggtcatccc tactttccct gcgagctgag
     2341 ggacttcatg ctcatgtcct cacggcccca ggccgccggc aactggtaag gctgctggac
     2401 gccggagtgc agatcgatat ggtctctcat caagatgagc gacccgcacc acgagaacat
     2461 tcccatctcc gggaaccacc cgtagttacc gtcccaggtg cggtagagct tccccttccg
     2521 gttgtagttc tcgatgtgaa ggaaattgaa cgtctcggca tcgacgtaga agatcctgtg
     2581 accgtagacg tagttcgggt cctgctgcgt cagtttcacc acgtagagcg gcctgcgctc
     2641 catcctcacg ttccggaact ctttcccctt ggaactgatg tactcggctc catccgccgt
     2701 tgccgccgga accaggtact ccctctcttc cagcacttcg tacttgtacg ggtaccgggt
     2761 cgccgaaagc ttctgcatcc acccttcgtt gtcgtcgtag atctggtcct ggcccatgac
     2821 ggggtcctgc gtatccgtgg ccgacatctt ccggacccgc cgcatggacg ggatgtacag
     2881 catgagctga tccgcggtgt tctggtcatt ggagtaaagg gcttgctggt acacgccggc
     2941 cacgtccctc ggtgcctgga agccgaacat gaaggtcttg aactcgttgc gctccttcgc
     3001 ccgcttgtcg aaccagccgt agggctccat cagggtgcgt cccgcaagcc ggacatgttt
     3061 cacgtcgtac ccgccgtcga agtccacgtt caagctgctg ttgacgccga tgacgtggct
     3121 gatgatatag aaattcagtt cccacgccag catccgcttc tcgatgttgt acatgatctg
     3181 ctgggccttg aaggggcccg aaggcttcgg gaaaggaacg ccgcccgccc aggtttcccc
     3241 caggaggtag cccttggcgt cgagcttcgt cttgcccagg ttcttcttcg tcgcctcggc
     3301 gatcggcagc gcccagtaca gctgccgcgt cgggatgatc tcaaactcgg ggatgttgcc
     3361 cccgtgcggc ggggctcccg gcttgatccg gttgtagagg tccgcccaca tgagcgcctt
     3421 aagccccggg ttcttcgcca gatccttgta ggtatacttc ccgggcttga tctcgggcgc
     3481 gatcttcccg acgatgtccg gagccttgaa tccaacgagt tccgcccaca ggctcttcat
     3541 ggagtcgaca tcgaaggaga gtttggcgta gaaatccttc ggaagaacct gcttcggccc
     3601 gaaggtcgtc aggtacgggc ggggatcgtc atacggggct ttgtgtttgt cgacggcgga
     3661 gagcgtcggc aagtaggttt ttggatcggg atattccgcc gcggacaagg tgaccgccga
     3721 cgtgatcagc acaacaagca caaagagcag cccccatttc gacaggccta actgcttggt
     3781 tatcctcata gcgttctctc ctttgccttg tggtagggtc atgacctaaa acctgtactt
     3841 tacggtggcg aacaactggt ccttgttctt caagggctcg aatccctttt gagggacttt
     3901 cccctccagg aacagcgccc ctatcgtgta atgccacttg tcgctgtaat cgtatgtgag
     3961 ctgtggcttg aacatgtttc cgttcaatgt catgtccctc atccagaaaa tctgcggcgt
     4021 caacttgtta tgaaaatacg ttgtgttgat caataacgtt gtcacatagt tgtctttctt
     4081 tatatttgcc aggaaatcct tcaagtccat ccccgcggaa acctcggcgg gtgccatgtc
     4141 cttatcgaaa tccagaatat ggcgatgata aaactgcccg gagatgaaga agaacgcctt
     4201 cggattcaga aaatccgcct tcactttcca gtccaaccca acgacatacc gtatctcgtc
     4261 cttcttgacg taactcccga acgggagccc gaccacccct cccgcggtgg acaacgtgtt
     4321 gttcatcacg tattcgcctt ccagacgaag gaccggtgcc actcctccca gagcgctgga
     4381 actcaccgcg gggaagtcgc tcgtgaaggt ggcgcccacc atctgatacc gggggaacct
     4441 ctcttccacg tgaaggtggg cgatgggcgt accgtccggg gccagcgtga tatccggtcc
     4501 actcagggac ctgagctgcc cgctattgga gaggccgttg aagtagttca gggagaagat
     4561 catgtcgtta accactccct tcacccgcgc cccgaactcc atccccgacc ggttccaatc
     4621 ctgcggcgcc ttgttggtgt agcgcgaaga gccgagctga accccgggcg ccacctgcac
     4681 gtccggagcc cagacccccg cgacgtcgtt gcccaggtcg atgggccggt ccgcctggaa
     4741 ccgggcgttc gggttaaaga cggtttcgag cccgatgtcc tggagccagc tcgattgggg
     4801 ctgcacgtag tagttcgccc ggacgagcca gatcgggatg atggtattct cgaattccac
     4861 atcccccagg ccccggcgct ggtccaccgg gttgatctgg tccatcaacc ggaacccgtc
     4921 cgtctctccc caacccacga tctgttttcc cacccgcagg aagaagttcc cgggcgacca
     4981 cgtgacgtac gcctcgttca gcatcttgtc ccagcccgtg tcgacgaaga gcgcatcccg
     5041 gctcttgttg aactccttgt ccttccattc actgttactg ctcaggatgg ggtaggccag
     5101 gtccgccgtg aattttcccg acatgaaaac cttcacgttt ggggcgggtt tatattcccc
     5161 ctccagcagc gacatgaaga ccgcggaatt catgccatcc ttgttgtcga agcttttgcc
     5221 attacggaag ctggtcgaca caccctgatc gacgtagccc atcagcgtgg cgggtttgtc
     5281 cagcaggttg aactccgtcg ccccgctcgg ggtttggctc ccaagcaatg ccaagacaat
     5341 caccgatccg atccaccgga accgtccttt ttccatcgcc tcttctcctt ttcctcccgc
     5401 ttgttttttc cgtcctttgt tcccgccccc cacgtccgtt tactcctgca acagcccgaa
     5461 actggcggac ggcgcagtcg ctgcttccgc ctcgaagttc cggctgatga atttcggttt
     5521 tatgatgtag atcatcaatg gatggagcgt cagcgccagg agcacgttgg ccgccatgat
     5581 gatcgacaga aagaagccca cttccgcctg gaacttcatg tcggagaaaa tgtaccaggt
     5641 cagaatgggg aggatgacgg tgatgccggt gtagaccacc gcctttccgc aggtgcagac
     5701 cgactgcatg atcgtcgccg tccagtcccc gtgctgcagg gggaactctt cgatggcgcg
     5761 gctatagaga tagatggcga aatcgacccc caatcccgcg ccgatcgccg cgatcggcaa
     5821 ggtgttaatg gagagcccga tgtcccggag cgacatgtag gtcgccgcca tgctgttggc
     5881 gaggaggagg ggaatggtga gcatgagccc cgcgacgatc gacctgtagc agaggctgca
     5941 cagcaccagg atgccgagat agacggccag atcgatcaga aggtgggagc gcttcatctc
     6001 ctcgttcacg gcgatctcca tcccgatccg gccgcccgcc aggtggaatt ccccgtgccc
     6061 gatcttcatg gaattggtcc tgaaaaaatc gtaggcggcg ttccggatcc ggagcaggtt
     6121 gtccgaggtg tgatccgcga agtacagcgt gatctgcgcc cgttccagcg tcgggtccat
     6181 gaaacgcccc aaggtgccac ggtccacggt attgcggatg taccccagga tcccggtgag
     6241 catctctttc tccagcggca gctggtacca gagcatgtcg ccatcgtgga aggtgcggtt
     6301 caggatgctg acgatgttca ggatcgacgt cgacgacttg tagatatccg gcagatgttc
     6361 cgccatatgg cgtgaaaacg attcgaaggt gttcaatacg accggctcgt agacggagcc
     6421 cgccgctccc tcatagtaga ggacgaagtt ctcggaggac gcatcgaagg tgttattgat
     6481 gacggcctgg tccttgttgt acggatggtt gctgaagaat acgggggagc ccgaagtggg
     6541 atcccctatc ttcaatttgc tcgtctccca gacgcagaag atcgtcagaa gaaggatcaa
     6601 cgcccccgtg atgtagcgcg tgttcgggcc gatggagaaa tgggtgatcc gcatcaggaa
     6661 cttcgccttc cggtcgagct ggcaactctc cttcgcccat tccgtgctct tcacttcctg
     6721 gggaacaagg ctgcagacga gagggacgag aagtcccgtg aagaggatcg tcgccatcca
     6781 gaagctcatg atgatcgcca ggtgaaccat caggacgatc ttggcgatga acagcaccgc
     6841 gaacccggcg acgtccgccg cgacggcggc gaagttgggg acccacatgg ttctcatggt
     6901 ttcataggac gccttcttcc ggtccccctt gctcgcatcg agttcctcga aatagcggta
     6961 ggcgatctgg tgggagttcc cgatcatccg ggccgccacg aggaaggcaa ggacgtagag
     7021 aaggggactg aagttgatgc cggtgaagcc gatgaatccc aggccccaga tcgtgagtat
     7081 tcccgcgttc gccagcgggg agatcatccc ggggaaattg tcgaagatga gccacagcac
     7141 cagcgcgatg gcgaggatgc tgaacgcaaa aaccatgtac atctggggcg taaggctgta
     7201 gatccagccc atcagtatgg ggaagcccac catgtggatc gatgtgttgt catcggagta
     7261 cttctgctgg agtccccgaa agagcgtgaa gatccgcgag taggagatgt tctccttgca
     7321 ctcggtgaca aggaggcagg ccgttccatc cttggagacg aggttgccgt tgtaggcggg
     7381 attggagaag acgttcttct tgagaagttc catggcttcc ggcgttttcg gcacgtccgg
     7441 gaacatgagc gattcgatgg tgatctcgcc cttccccttc gcgttcacca ccttggtgga
     7501 gttgctggcc atcgagaccg tgaggaccct gtagacctcg tcccacagct ccacttcact
     7561 tgtcatgttt ttgatcttcg tgagggtctt ctcgttgaag atatccccct tcttcacctt
     7621 cacggcgacc accacgccgg agccgccgcc gccgaagacc tgcccgaacc ttcccatgag
     7681 cttcaggtag gggtgatcgt agggaaacat ctccgacaga atgacttccg tatgtatcct
     7741 ggagatcccg accccgaaga cggcggtgat ggccagcacg agcacgagga tcttgatctg
     7801 gtgctgcgcc agataccagc agaatcgttc aaacctgctc ttcgtttcca agatcattgc
     7861 tctcctttag gtgttcatta atgacgcgct gctatcgggt ccccacacgc ttccactccc
     7921 ccgcaccggc gtccgcgccg tagatccatc ccgcctgccc gaccgccacg aacccgtcct
     7981 tcccccgttt gccgatccgg tagagccacc cgtaccggat cgacggctcg accttgactt
     8041 ccttccaggt ctccccgcca tcggcggtcc gcagaagaac ggcatccccg gcgatcagca
     8101 cgtttttctc ccccgcggaa acgccgaaga tgtgcgtctt cggcacccct gtcatctgct
     8161 tgcgccatgt cttgccggcg tccaccgtct tcgccacata accgtcgatt cccacgaccc
     8221 acgccgtcat cgggttcacg gcgacgatgt cgaatagata gctcccgccc acaacctctc
     8281 ccgtctcatc cgaaaatccg aactcccccg cctggtgcgt ccaggtcccg ccgccatccg
     8341 acgtgtggta gacgaatccg tactccccca ccgcccaacc gttcctctcg tcgcaaaacg
     8401 acacggattt caggatgaag tcctggtcct tgaattgaac cgcccacgtc tttcccccat
     8461 cctctgtatg aagtatgttg gtgcgctcgg tgacgatcca cccctggttc ttcgtcgcga
     8521 aagagactcc catcaggaaa tacgtgaccg ggcttttctg cttctcccag gtcttgccgc
     8581 cgtccgaggt gtggatgatc gtcccttgat ccccgacggc ccagccgttt ttttcatcca
     8641 cgaaactcac ggacgtgagc gtgtaattga ccccagtgcc ctgcttttcc caggtcttcc
     8701 cgccgtccgc ggtgtggagg acactccccc accgtccgac cgcccacccg tgtttctcgc
     8761 tgccgaagtt gaccgagaac agatcgtccg tcatccgctg ccccacgaat gccggggaaa
     8821 cgaggggaac acagatcaat gccgccagaa tgaaaacgat cgacatgcgt ctcaaggcct
     8881 acctccgtaa gcataagtgt tctcttgccg gtcttccccg aaccggtttc ggaaaacgaa
     8941 cttggcactc cgatcacgac cgtaatttac ggctcttcaa aacaattatt tatttacgaa
     9001 cagatccatt atgaagggcg ccttaaagca tgaccatagc gggaatccgt caattttcat
     9061 ggacatgttg ccgcagatcc agatgcccgc ggtaacctcc cggaggaaag ttactgggga
     9121 acggcggtcg tcacggcaga ccggatcgga aaccggcttc cgctatctct gccccgtata
     9181 aagcattact ttgcggagca agttgatacg gttcttgaca ttcatttttt cgtaaataga
     9241 cctgagatga ttttcaacag tatattcgct gataaacaat ttttcaccga tctcactatt
     9301 tttgagacct tcacagataa gatcaataac ctcctgttca cgccgggtaa tcctcaattt
     9361 atccaactgc tttgacaaaa gcaccccaga ctcattttta ttcatgcaaa tcaggcacat
     9421 cttggaatcc ccgaaataac ttatcatacg aaaatttgct gatatggttt tcccggaact
     9481 cgcattcaac agctttactt ctctgctcgt cgcagtctca ccgtttagtt ttaaactttc
     9541 cataaattgt ctacactgaa catataattc catcggcaaa gcaaacgctg atttttccga
     9601 tggttcattt tttcctacaa aataggatat agtttccatg gcttcttcgt ttatgtaaac
     9661 cggatcaata gattgatcaa gaatcaaaat tcctttatac ggtaaatcca tacaaaaaga
     9721 actcataatt ttttctgact ttgttatttt ttctataaat atagatttct ccagtgctcc
     9781 tccaagggaa gaggagataa gttcaccctt tgccttttcg aaacgggaaa aatcttgctc
     9841 gtgttcggaa cgaaataagg ccaacgtgcc acacaattgt cctcttgatt ttagaagtat
     9901 taccatttgc ttatagatac actgtggctt aagaaaatca ttgaagtact cacttcgtat
     9961 catgtcttta ttggaaataa tactactggt agtcataact gtctcggagg cgcttatggc
    10021 ctttaaaaat ggatccatct tgtaatagtg ttgtcggaag agatccagat atttttggtc
    10081 gatcccctga gttataacgt tgccgaggtc aagccttgga gaggtatatt ggcggaacag
    10141 gaaaaaattg caccgttcga catgaaatgc ctctacaagt ggtttgagta cctctttccg
    10201 taaatcatct atctggtcac attccagcgc agtgcgaacg atcttgatga catctaacat
    10261 gtcctgcgat ttaagcatta acggataacc caccctataa aataaaatgc catttgcatt
    10321 ctaacacagg ccttggtctc gatgtccttg cggagaagtt cgaccacccg tggtggccta
    10381 ggctccttgg gcaattttgg ctttccccgc ggcagcggca tggcccttac cactccgccg
    10441 gtcggcaccc gacgcgtcag tgcgccgatg ttgccgtttt caagcgctcc cttgggaccg
    10501 agggcgacct ctaccgtccg ttcgcgatgc tcggccgtcc gggcgccgtg gcggacctca
    10561 aaagcggcgt ggacgatccg gggacagatc tcaggctcgc cccaatccgg gcgggttcgc
    10621 atattgttac atccgaggtg ccatatggga ctcgcccccc cctgctgttt catcaacaag
    10681 ccaatacgat catcgtgaac gacttcgtgt ccccgtcctt gtcgccgccc atgcagtgag
    10741 cgagcccaac cttcgcgttt cggacctgac ggtcccccgc ctcgcctcga agctgggtca
    10801 cgatttcgtg gacttgagcg agccccgttg cgccgggggg gtgcccccgg ccgatcaacc
    10861 cacccgagac gttcacgggg atccggcctc cgagccgcgt ttccccccgg accgcgagat
    10921 tcgcgccctc tcccgggccg caaagcccca aggcctcgta atgcaggatc tccgcgatgg
    10981 tgaacgcatc gtggcattcg accacgtgga cgtcctccgg cccgatgccg gccgcctcat
    11041 agcccttggc gcagccccgg tagtctgtct cccagcggtc gaggtcggcg ggattgtcat
    11101 agctgccggt gcacagaatc gccgccttca cttcgacagc gcggcgcctt cgcctcgctt
    11161 cctcctgcga gcagagcacc acggccgcgg cgccatcgga catcgggcag cactgcatgc
    11221 gcgtgagcgg atccgccacg accggggacg agaggacctc ctcgagcgtg acggccttcc
    11281 ggaaatgggc gtacgggttc aggacggcgt ggccccggtt cttcacggcc acggacgcca
    11341 gttgcgcggc gctggccccg aacttgtgga tgtatctggc cgcgcgcatc gcaaaccccg
    11401 ccggcgtcac catccccaac cgggtgtcga gctcggtctc tccggagttg aggagcccca
    11461 cccccggtat gcaaagcttt tccgcaccga cgacgagagc cacgtcgacc tgtcccgcgg
    11521 caatggcgtt acaggcgaga acgaaagccg tcgagcccga ggtgcacgca ttctcgacgt
    11581 tcaggatggg gatacgattg atccccactt cccagaagat gttttgcccc gacgtcattt
    11641 ctccgaggag ccgaggggcc gcggcggagg cgaagacgcc aaaccccacg cggcctggct
    11701 cgatgcccgc atcctcgagc gccaggtacg cggcctcgga gcccatgtcg acgagggtcc
    11761 gctcaacgtg cttcccgaag gggatcaacc ccacgcccgc gacgcatgcg gcgcccatca
    11821 cgcaactcct cccggctcag gaaccaaggg tgtgaaatac ggacggagac acgacgctcc
    11881 acgggcgtcg ttccccaacg ctccgacggc caggcgtacc ggaatcccga tgcggaattt
    11941 ctcaacggcc tgcggatcca gaagggagaa gacccgaagg ccgctctcct tcagatccac
    12001 atagccgatg gcatacggct ccggaaggcc caacggcggt cggattcgaa tgacggtgaa
    12061 gctgtagacg gtgccctcgg ctcccaccgt tgacgcttcg accggaccca ggcatctgcg
    12121 gcaccgggat gggcggggga aataccggac cccgcaggag gggcaggagc cgccgaggag
    12181 acgcggttgc ccgccatcgg ccgggatgtc gaggatcccg ggcatcacga cgggcggcaa
    12241 tacggcctcc tcgatgttcg gctcttcctg tctcgtcacc ttcatcctcc agatcgatgg
    12301 cgcttccgag aggcgtcctt cccctcgagg tcagcgcttc agctcaagtc ccgcctttac
    12361 ccggtcccag gtgtccgggg tgatcccctg cttgcgaagc tcggacttct ggaccttttc
    12421 cgtggaagtc ttgggcattc gctccatgat gcgaacgtag cgggggacca tgaagtaggc
    12481 catccggtcc cggcaatgcc cgaagagttc ctccggccgc aatggaatcc cgggcctggg
    12541 ggtcaccacg atcatcacct cgtcctcccc catcccgtcc ttgacggcga tggccgcgga
    12601 ctccaggacc gcggcgtggg cgttcacgac cttctccact tcgtaggagc tgatgttctc
    12661 gccccggcgg cgcaacgcgt ccttcttccg gtcgacgaag tagtggaaat cctcttcgtc
    12721 cttcaccgcg tagtcgcccg tgtggaacca gaggtccctc cacgcctcca cggtctgctc
    12781 cggcatcttg tagtactcga ggagcatccc gtaaggctgg gagggccgga cgaggatctc
    12841 tcccacgacc ccggggcccg cctccgcgtc ggcgtcgtcg acgagcctca cctggcaccc
    12901 gaaggcgggt cggccgatgg agccgggctt ccggtcatcg atcgtgttca tcagcggcag
    12961 gccgatctcg ctcatcccgt agccttccag gatcttcagg tcgaaccgct tctccaccgt
    13021 ctcgaagagg tccttcgtgc agccggctcc cagcatcacg cggatcgggt tgtcgacgtc
    13081 gtcttgcctt ggctccgcct tgaggaggat gtgcaggatc ccgccgatgt agttgaactc
    13141 cgtgcagccg taccttcgca cctcgctcca gaagctcgac gcggagaagc gctcggccag
    13201 gaccattcgc gcgccgctca tgagggcagg catggtcgac aggacctggg cattgccgtg
    13261 aaagagagga agcgcgttgt agaggcaatc ttccccggtg taccgcgcgg accgcgaaat
    13321 ggtctcgccc atgaggaggg catagttctg ggggagtacc gggcccttgc tggggccggt
    13381 ggtccccgag gtgaacatga tggcgaaggg atcggaccag agaagttcgg gcgcgtcgta
    13441 gcggccgtcg ttttccgtga cggagcgata gtccgtcgcc ggccggtcca gggcggacag
    13501 cgcctcgccg ggttgtccga gcaccacgac cctctccacc cccgggaggt ccttcagcac
    13561 ccggaccacg gggtcgaggc atgcggactc cgcgacgacc atgcggcaat cggccttgtt
    13621 caccatgtac gcgagaatgt cgccccggtg cgccgtattg atggggacct ccaccgcccc
    13681 gagcttcgcg agcccgaacc acaggaagag gaactccggc cggttgccca tgaggatcgc
    13741 caccttgtcg cccttgccga tgccgaggga ctggagacct gccgccacct ggtccgaagc
    13801 gcggtcgaaa tcccggtacc cgaactcctg ctccctgaag tagaagaagg tgcggttccc
    13861 gaactgcttc accttttcgc gaagcacgtg ggggatggtc cgtgcatgca cccggtcggc
    13921 cgatcccatg tcctctcctt ccactcctct tccgtcgatt cagatcgcgc cggagaccgg
    13981 ataggtcccg tagtacttga tgacttcgtc cttgggccga agcttcagcc actccttctc
    14041 gaactcgccg tacggttttc ccctctgctt ccgccgggcg cgcgcctctt cccgcagctc
    14101 ccgggtcttc tccctgtcga gccgaagcgt ctcgtcgtcg taggccaccc ggtagatgtt
    14161 tcgggccgcc cagggagtga ccatgccgtc ccgcaggtcc gccaccaccg cttcgggatc
    14221 gcgttccaag gcatccccgt atcccgcgcc gccaccgacg ggaagataaa gagtgtcgcc
    14281 gttctgaaac ggctggaccc gcatgctgct gtcatgaagc tcgcgccgcc cggtctcggc
    14341 gccattatcc tggagcgcct gctccacatt caccggcacc gaagggctcc cctgggcaag
    14401 aaggtccttc atgttgctgc ccctgacggt ctggacgaag gggggcggca cggcgtagcc
    14461 gccgaagatg cccaccgtcg ccgggaacct ggatccgtaa ccgaatgcac ccaggcccac
    14521 cccgggaacg tggaacggca tgatgcagta gccgcttccc gcgccgccgc gatacttgcc
    14581 gtggccgtac gacccgcgaa agtagttccg gaagaggtac aggaacggcc tgtcttcctc
    14641 ggtagtctcc acgtcgccgc aatcgctcat ggtggcgaag aaggaaccgg cgcagtcgac
    14701 accgtccatg tcgggcctgg cgccgcagcc ggtggagttg atctccgcga aaatatcggc
    14761 catcggctcc ccccactggt tgattccccc gaacatcggc acgccgaatc ccgtgtacca
    14821 ggcggccccg gcgcgcagcg gatcgaggct gtagctcatc cgggcgccgc acaggaacat
    14881 gccctgggaa aaggccgtct ggaggaaggg agcgaggctg gtggaaacct cgccctttgc
    14941 gttgatgagg gagtcctcgg ggaagtgcca ctcgatggtc tcaaggaggc cgttgttcgc
    15001 gggcaggtca tggaagaacc atccgcagaa gtacaccatc gccagcccga ggatcccgtg
    15061 aaagtacgta ttgagcggct tgtcgggaat catcggagag cttccttcga agtcgaagaa
    15121 gaagccgtgg cccttcttcg tgatcgtgag attgatcttc accagcgtgc tctccgtgcc
    15181 gacggtgtcg agaaaccggg gctgccggta gacgccgtcg ctcagaaggg cgattttctt
    15241 cttcgccgcc tcggcggtga ccgagagaat cctgcgcaga ctcccgatga agaattcctg
    15301 gcccttgtct ccgaggacct cctgaatccg ccgctccgcg atccgggaag ccgcaaggcg
    15361 ggccttgatg tcgaggatca tcgtcctcgg gtcgcggacg tgggacccga tcatcgtcag
    15421 gatgtcttcc ctcaaggcgt agcgctcccc gaccttgatg ggggggcaga gcagtccgtc
    15481 gtcgtaccgg gagcgagcgg tgttgctcaa cccgcccgga tccgtccccc cgttctcacc
    15541 cgtgtggacc acggcgccca cgaagcagac gagctttccc tggtggaaga cgggcacggc
    15601 caggcccatg tcggcgccgt ggacgccccc gtagaaaggg tcgttccaga agaaggagtc
    15661 cccctcgtcg accttcaccg aggggtcgtt tacgaagtgc ttcagaatgt acttcaccgg
    15721 gatctggccg aggacggcat ggaaccagat ccccgtcgcc accaccgcca tgtctccctg
    15781 ggcggtgtag atggcgaagg ccacatcgcc ccagcgcatg ccggtagacg cgcccagttt
    15841 catggtggtt tctttccctt cctgggcgat catgttcatc ttgtggcgga agatctcgaa
    15901 gtcgatgccc tcgatcttct cctggcccgc cgcctcttcg gcggtcatcg gttccgggcg
    15961 gagccgctgg acgacctcgt aatccctgcc gtacgccagg ctgctcatcg ctttttctcc
    16021 tcttccgccc gaaccgccgc caagaccggc tccaggatcc cgtttcggta ggggtcaagg
    16081 gtgaaacgcc aaccgggttc gatcacatag gttgtatctc gcgcctcgac gagcgccggc
    16141 cccgtgagcc tgttcccggc ctcgagagcc tcgaacacga agaccggcgt ctcgctcggc
    16201 ccgccgccgg accagtgagc cttgcggctg tcggccctcg cggcgtcggg cggcgccaag
    16261 ccccggaggg gaagcgcttc cggcttgtgg agacggtttt gaaccgacgc cgtcaggtag
    16321 aagcactcga tgttgattcc tccccgcggg aaggtcgcct ccggcgagta ggtggcggaa
    16381 tactgttcgg tgaaccgatc gcagatctcc ttgaactcgt tgggcccgtg gatggccaga
    16441 tacggcgaaa cgaccttcgt gaggttgtac tgcatgccgt accgcatctc gatctcgagg
    16501 cggaactcga cctgatcttc gctgaaccct tcgagcctga agtcacgaag cgcaaggtcc
    16561 ttgagctcgc acacgacggc gttgaacccc tcggcgtccg cggagtaggc ctgatcggcc
    16621 cagcggaaga tcttcatcgc cttcgatttc tcccagacct gccgcacagg cagcgtgtac
    16681 gccccaaaag ccccgaagac cggcgagaag ggcgacgtga tgaccctctt cacccccacg
    16741 taaggggcaa acccgcaggc gtgagtcggg ccggcaccgc cgcaggcgta gaggtcaaac
    16801 cggcgcgggt cgaggccctt cacgacgacc tcgctgaaga cctcctgtcc catcctggcg
    16861 tcgacgatcc ggcgtattcg aagggccgcc tcgaccacgc cgatccccaa aggtttggcg
    16921 atcttcttct cgatcacctg tgcgctcagg tccggatcga gcagcatctt tccgcccagg
    16981 tagttgtccg ggttcagata cccgaggacg aggtcggcgt cggtgaccgt cgggtcctga
    17041 ccacccttgt cgtagcaggc gggcccgggc atggagccgg cggattgggg cccgacctcc
    17101 aggctccctc ccaggagctc gttcacccat gcaatcgatc ccccgccggc gccgatcgat
    17161 ttcacctcga tggcggggat gttcgtgcgc catcgatcga tcacggggat gaagtcgtag
    17221 gtgctcaagc gcccccccgt gatcacaccg atgtcgaagg acgtcccgcc catatcggtg
    17281 aagatcacgt tctgttgacc gtatctggct ccatccgtaa gggatccgtg gagaccggcg
    17341 accggtcccg cgttgtgggt cagcacggct cgcgtgcggg atgccttttt catcccgccg
    17401 gtgttgtgga cgagcagcag cggccttttg tagccgccgt cgcgaagctc attcaccagg
    17461 tggccaagct gttcgcccat gattccgtga atgtaggcgt ttacgatcgc ggtggtga
//
LOCUS       DASVJL010000126         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1520121, whole genome shotgun sequence.
ACCESSION   DASVJL010000126 DASVJL010000000
VERSION     DASVJL010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520121"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VF357_02500"
     CDS             complement(<1..621)
                     /locus_tag="VF357_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523045.1"
                     /translation="MLDVRKIDTYYGQSHVLFDVSLSLRQGEIVGLLGRNGAGKTTTM
                     KTVGGMLRPKKGTILFEGEDVTGRKPFQLVRRGICYVPDDRRIFSDLTVDDNLGIVYR
                     RTREWDRERVYDLFPALREIADRRAGVLSGGEKAMLAVGRALMSGPKLLLLDEPTEGL
                     APLIVRALEEQILRLKGAGISILLSEQNLKSSLRLIDRAYIIDNGRI"
     gene            complement(615..1358)
                     /locus_tag="VF357_02505"
     CDS             complement(615..1358)
                     /locus_tag="VF357_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523046.1"
                     /translation="MLRVESVVKSFGGFKAVDRANLHVDAGEIVAVIGPNGAGKTTLF
                     HLLTGHLSPDSGRILFKGKEIGGLSPHVICRRGITRSFQVVNIFPRLSVFENVQIAVL
                     SRERKTFRIFSPVKGMAEEEVHRILRSVGLFDQRDVESDLLSHGDKKVLEIAVALSGN
                     PELLVLDEPTAGMAPEETERCIRLIRKLSEESGLTILFCEHDIALVFEIANRIMVMVR
                     GATVVQGSGEEVRRNSEVQSAYLGESGEC"
     gene            complement(1351..2286)
                     /locus_tag="VF357_02510"
     CDS             complement(1351..2286)
                     /locus_tag="VF357_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7523047.1"
                     /translation="MRRSAPPLLLLLLLATLLALPLVLPRFHTYVAAIVLLTALLATS
                     LNLAIGYTGLYQFGHAVFYGVGAYGTALMLTRSGMPAPVAFLVGPLAAAVLSLVMGLI
                     CIRLSKLYFGMLQISLGSLVWAVVYRWYSFTGGDDGIHGIPLPDAIASPKGSYYCTLI
                     VTTLCVFAMYRIVRSPFGKVIQGIRDNPVRAAAIGVNVKLHQLASLVIAGFFGGVAGS
                     LFVVVENSVFPDMMFWTFSLEVLIMCLLGGMYTFFGPLVGASVIVLLRVFASVHTQYW
                     GLILGTILTLVIIFLPDGVLGVFTRRSLPKEDRGA"
     gene            complement(2297..3193)
                     /locus_tag="VF357_02515"
     CDS             complement(2297..3193)
                     /locus_tag="VF357_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7523048.1"
                     /translation="MDFAANLFSTGTLSQILVGLSRTTILFIVSSGMSLILGVLRIPN
                     VAHGSLYMIGAFAAWTVIHFLGGGLFAFLAAMIVAPLIVAAVGFVLERGFFSYLYERV
                     HLMLLLLTFSFALILGDLVKIVWGPEYRSVTVPAFFQGSAEIFGLPLPWYNLFLLLIG
                     PCIAVFLWLVINRTNLGKIARAAAVDGEMVGAVGINVGWIFAFTFVLGSFLAGLGGAL
                     IAPMVNISLGMDHSLIMETFLIVIIGGLGNMWGALLGSLIFGLTQSLGILVWPQFGIA
                     FPYLAVVAVLVIRPRGLLSSTW"
     gene            complement(3206..>3693)
                     /locus_tag="VF357_02520"
     CDS             complement(3206..>3693)
                     /locus_tag="VF357_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7523049.1"
                     /translation="STGFNKKVPFYQHTAIELSTLMPQGQNAPEGVYGTANYLFYYPD
                     TKENKAFVAEFRKAYGRYPKSGALYGYMTAQFIAKSFEKAGKVDREKFIDAMEGMTLP
                     SPVGNMEMRACDHQVTLPMFFGVTKKSTQYDFLIASDVVSVPGKDYMPSCAEIAKARA
                     K"
BASE COUNT          727 a         1209 c         1171 g          586 t
ORIGIN      
        1 gatcctgccg ttgtcgatga tgtaggcgcg gtcgatgagt cgcagcgacg acttcaggtt
       61 ctgctcggag agaaggatgc tgatccccgc ccccttcagc cgcaggatct gctcctcgag
      121 cgccctgacg atcagcgggg cgaggccttc cgtcggctcg tcgagcagga gcagcttcgg
      181 gccgctcatg agcgcccgcc ccaccgcgag catcgctttt tcgccgccgc tcaagactcc
      241 cgcccgccgg tctgcgatct cccggagcgc cgggaacagg tcgtacaccc tctcccggtc
      301 ccattcgcgg gtcctccggt agacgatgcc aaggttgtcg tcgacggtga ggtcggaaaa
      361 gatccgccgg tcgtcgggca cgtagcagat gccgcggcgg accagttgaa acggcttccg
      421 acccgtcaca tcctcccctt cgaagaggat cgtccccttt ttcggccgga gcatgccgcc
      481 gacggtcttc atcgtcgtcg tcttccccgc accgttgcgt ccgagcaggc cgacgatctc
      541 tccctgccgc agcgacaggg agacgtcgaa caggacatgg ctctggccat aataggtgtc
      601 gatcttacgg acgtctagca ttcgccgctc tcaccgaggt aggcgctctg gacctcgctg
      661 ttgcgccgca cctcctcgcc gctcccctgc accacggtcg cgccccgcac catcaccatg
      721 atccggttcg cgatctcgaa cacgagcgcg atgtcgtgct cgcagaacag gatcgtcaac
      781 ccggactcct cggagagttt ccggatcagg cggatgcacc gctccgtctc ctccggcgcc
      841 atcccggccg tcggctcgtc gaggacgagc agttccgggt ttccgctcag cgcgacggcg
      901 atctcgagga ccttcttgtc cccgtgggag agcaggtcgc tttcgacgtc cctttggtcg
      961 aacaggccca cgctccggag gatccggtgg acctcctcct ccgccatccc cttcaccggc
     1021 gaaaagatcc ggaacgtttt tcgctcccgc gaaaggaccg cgatctggac gttctcgaag
     1081 accgagagcc gcggaaagat gttcaccacc tggaaggagc gggtgatccc cctccggcag
     1141 atgacatggg gggaaagtcc gccgatctcc tttcccttga acaggatccg ccccgaatcg
     1201 ggagaaaggt gtcccgtgag aaggtggaac agcgtcgtct tccccgcgcc gttcggcccg
     1261 atgacggcca cgatctcccc cgcgtcgacg tgcaggttcg ccctgtcgac cgccttgaag
     1321 ccgccgaacg acttgacgac cgattccacc ctaagcaccg cggtcctcct tcggaaggga
     1381 ccgccgggtg aacactccga gcacgccgtc ggggaggaag atgatcacga gcgtgaggat
     1441 cgtgccgagg atcaggcccc agtactgggt gtggacgctc gcgaagaccc ggagcagcac
     1501 gatgacggag gcgccgacga gcggcccgaa gaacgtgtac atccccccca gcaggcacat
     1561 gatcagcacc tcgagggaaa acgtccagaa catcatgtcc ggaaagaccg agttttccac
     1621 gacgacgaac agcgatccgg ccacgccgcc gaaaaacccg gcgatcacga gggacgccag
     1681 ttggtgcaat ttcacgttca cgccgatcgc ggccgcccgg acgggattgt cccggatccc
     1741 ctggatcacc ttgccgaagg gggaccggac gatccggtac atcgcgaaaa cgcagagcgt
     1801 cgtcacgatc agtgtgcagt aataggaccc tttcggagag gcgatcgcgt cgggaagcgg
     1861 aatgccgtgg atcccgtcgt cgcctcccgt gaaggagtac cagcggtaga cgacggccca
     1921 gacgagggat ccgagggaga tctggagcat cccgaagtag agcttcgaca agcggatgca
     1981 gatcaggccc atcaccaggc tcaagaccgc ggcggcgagc gggccgacga ggaacgcgac
     2041 cggggcgggc atcccgctcc gcgtcagcat cagggccgtt ccgtaggcgc cgaccccgta
     2101 gaacacggcg tgaccgaact ggtagaggcc ggtgtagccg atggcgagat tgaggctcgt
     2161 cgcgagcagg gcggtaagca ggacgatcgc cgcgacatag gtgtggaacc gcgggaggac
     2221 cagcggcagc gccaggagcg tcgccagaag gaggagcagg aggagcggcg gggccgatcg
     2281 cctcatcgac gcacccttac catgtcgaac tcaggagccc tcgcggccgg atgacgagga
     2341 cggcgacgac ggcgagatag gggaaagcga tcccgaactg cggccagacg aggataccga
     2401 gggactgcgt gagcccgaag atcaacgaac cgagaagcgc tccccacatg ttcccgagac
     2461 cgccgatgat gacgatgagg aacgtctcca tgatcagcga atgatccatg ccgagcgaga
     2521 tgttgaccat cggggcgatc agcgcgccgc ccagcccggc gaggaaggac ccgagcacga
     2581 aggtgaacgc gaagatccat cccacgttga tccctaccgc gcccaccatc tcgccgtcga
     2641 ccgccgccgc gcgggcgatc ttccccaggt tcgtcctgtt gatgacgagc cagaggaaga
     2701 ccgcgatgca cggcccgatg aggagaagga acaggttgta ccacgggagc ggcagcccga
     2761 agatctccgc cgatccctgg aagaaggccg gcacggtgac ggaacggtat tccggtcccc
     2821 agacgatttt caccaggtcg ccgaggatga gcgcgaagga gaaggtgagg agcaggagca
     2881 tcaggtgcac ccgctcatag aggtaggaga agaacccccg ttccaggacg aatccgaccg
     2941 ccgcgacgat cagcggggcg acgatcatcg ccgcgaggaa ggcgaacagg ccccccccga
     3001 ggaagtggat caccgtccag gccgcgaagg cgccgatcat gtagagcgag ccgtgcgcca
     3061 cgttcgggat ccggagcacg ccgaggatga ggctcatgcc ggacgacacg atgaacagga
     3121 tcgtcgtccg gctgagcccc acgaggatct gggacagcgt cccggtggaa aacaggttcg
     3181 ccgcgaaatc catcgggtgg agcggctact tcgcccgtgc cttcgcgatc tccgcgcagg
     3241 aaggcatgta atccttcccg gggaccgaaa cgacgtcgct cgcgatcagg aagtcgtact
     3301 gcgtcgactt cttcgtcacc ccgaagaaca tcggcagcgt cacctgatgg tcgcacgccc
     3361 gcatctccat gttgccgacc gggctgggaa gcgtcatccc ctccatggcg tcgatgaact
     3421 tctcccggtc gaccttcccg gccttctcga acgatttcgc gatgaactgc gcggtcatgt
     3481 agccgtacag ggcgccgctt ttcggatacc ggccatacgc tttccggaac tccgcgacga
     3541 acgccttgtt ctccttcgtg tccgggtagt agaagaggta gttcgccgtc ccgtacactc
     3601 cttccggcgc gttctgcccc tgcggcatca gcgtcgacag ctcgatcgcc gtgtgctggt
     3661 agaaggggac cttcttgttg aaccccgtcg att
//
LOCUS       DASVJL010000127         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_863973, whole genome shotgun sequence.
ACCESSION   DASVJL010000127 DASVJL010000000
VERSION     DASVJL010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863973"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VF357_02525"
     CDS             complement(<1..530)
                     /locus_tag="VF357_02525"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX7523050.1"
                     /translation="MKLPELTIGRFKAKVPIVQGGMSVRVSTSSLAAAVADCGGIGTI
                     GGSGIPIDELKEDIRKAKRMTNGIVAVNIMFAVKQFMEAVKASIEAGVDMIVTGAGFS
                     RDIFKVGKDHNIPIVSIVSSPEFGKLAERSGADAIVVEAKEAGGHLGTDRPLRELFPE
                     VRKVVKKVPLIAAGGIT"
     gene            complement(616..1605)
                     /locus_tag="VF357_02530"
     CDS             complement(616..1605)
                     /locus_tag="VF357_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7523051.1"
                     /translation="MVSSTAPPLVPDVPVSTSLQTPLRGTPPLRHSCFDTFTGDGMSE
                     RRILALKVDVDTRRGMEEGVPSLLATLDAFHVSATFFLSFGPDNSGKAVYQLLRNPRF
                     LVKMLRTNAPGLYGFRTALYGTLLPAPMIACALPGLCREIEARGHEVEFHAWDHRAWQ
                     DTLPRKGAAWIREWFLRGLAAYEEALGHRPRAFGAPAWLLTEEAVSVLREFPLEYLSC
                     TRAAAPFVLEGTGLVEVPSDLPCLEEVGGGNGVPRILSALDAGGVHVFPVHAEAEGGI
                     WRGAFAEILRGASDRGYAIHPLSRIAADCRGEVLPGRPFRTALLPGRAVPCSV"
     gene            complement(1693..>2165)
                     /locus_tag="VF357_02535"
     CDS             complement(1693..>2165)
                     /locus_tag="VF357_02535"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7523052.1"
                     /translation="IETAKEGSSRVVTQFIADLYLGKPIQVVDGGEQRRCFTYVDDGI
                     AGLMKILANRNGCANGGIFNIGNPANDHSIRELAEMLRELYAKHPRNRGKKVPPIIEV
                     NSKQFYGDGYQDIQTRTPSIRRAKEILGWEPKVPMKTALKKTLDAFLAEADASR"
BASE COUNT          375 a          727 c          698 g          365 t
ORIGIN      
        1 gtgataccgc ccgcggcgat cagggggacc ttcttcacca ccttgcgaac ctcggggaac
       61 agttcccgca gcggccggtc ggttcccagg tgccctcccg cctccttcgc ctccacgacg
      121 atcgcgtccg cgccgctacg ctccgcgagc ttgccgaact ccggggagga gacgatggag
      181 acgatgggga tgttgtgatc cttccccacc ttgaagatgt cgcgggagaa cccggcgccg
      241 gtgacgatca tgtccacccc cgcctcgatc gacgccttga ccgcttccat gaactgcttg
      301 accgcgaaca tgatgttgac ggccacgatg ccgttcgtca tccgcttcgc cttgcggatg
      361 tcctccttca gttcgtcgat cgggatgccg gagccgccga tcgtgccgat cccgccgcag
      421 tcggcaaccg ccgccgccag cgacgacgtg gagacacgga ccgacatccc cccctggacg
      481 atcggtacct ttgccttgaa ccggccgatg gtaagttccg gaagtttcac gatccccctc
      541 caacccgagt atataaactt taattataaa cgataacgct gttttgcatc ttcgaaaatc
      601 gggaatgaac cgaaatcata cggaacaagg gacggcccgt cccggcagga gggccgtccg
      661 aaacgggcgg ccggggagca cctctccgcg gcagtcggcc gcgatccggg agagaggatg
      721 aatcgcgtag ccgcgatcgg acgctccgcg caggatctcc gcgaaggcgc cccgccagat
      781 cccgccttcc gcctccgcat gcaccgggaa gacgtggacc cccccggcgt cgagcgcgga
      841 gaggatccgg ggaaccccgt ttccgccccc gacctcctcg aggcacggca ggtcggaggg
      901 tacctcgaca aggccggttc cctcgaggac gaaaggagcg gcggcccgcg tgcagctcag
      961 gtattcgagc ggaaactccc gcagcacgct caccgcctct tccgtgagca accacgccgg
     1021 cgccccgaaa gcacgcgggc ggtgtccgag ggcctcctcg tacgccgcga gtccccggag
     1081 gaaccactcc cggatccacg cggccccttt cctcgggagg gtatcctgcc atgcccgatg
     1141 gtcccaggcg tggaactcca cctcgtgccc gcgagcctcg atctcccggc aaaggccggg
     1201 aagggcgcag gcgatcatcg gggcgggaag gagcgttccg tacagcgcgg tccggaagcc
     1261 gtacagtccg ggggcgttcg tgcggagcat cttgacgagg aagcgcggat tccgcaggag
     1321 ctggtacacg gcctttcccg agttgtcggg gccgaacgag agaaagaacg tcgcggaaac
     1381 gtgaaacgcg tcgagggtcg cgaggagcga cggcaccccc tcctccatcc cgcgccgcgt
     1441 gtcgacgtcg accttcagcg cgagaatccg acgctcactc atcccatcgc cggtgaacgt
     1501 atcgaagcag gagtgacgca gcgggggggt tccgcgcagc ggcgtctgga gcgaagtgga
     1561 gacagggacg tcgggaacga gcggaggcgc agtcgaggag accatggaag gtcgacgaga
     1621 cgtagtggag cggaagcccc ccgcgaggaa ccctgcgccg tacgatcttt ccgtgtttgc
     1681 ggatcaagcc ctttaccgcg atgcatccgc ctcggcgagg aaggcgtcga gggttttttt
     1741 cagcgccgtc ttcatcggca ccttcggctc ccacccgagg atttccttcg cgcggcggat
     1801 ggagggggtc cgcgtctgga tgtcctggta cccgtcgccg tagaactgct tcgagttgac
     1861 ctcgatgatg ggggggacct tctttccgcg gttccggggg tgcttcgcgt acagctcccg
     1921 gagcatctcg gcgagctccc ggatcgagtg gtcgttcgcc gggttcccga tgttgaagat
     1981 cccgccgttc gcgcagccgt tccggttcgc gaggatcttc atcagccccg cgatcccgtc
     2041 gtccacgtag gtaaagcagc gtcgctgctc tcctccgtcc accacctgga tcggcttgcc
     2101 gaggtacagg tcggcgatga actgcgtgac cacgcgggag ctcccctcct tggcggtctc
     2161 gatcg
//
LOCUS       DASVJL010000128         5499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1613345, whole genome shotgun sequence.
ACCESSION   DASVJL010000128 DASVJL010000000
VERSION     DASVJL010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613345"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1852
                     /gene="lepA"
                     /locus_tag="VF357_02540"
     CDS             <1..1852
                     /gene="lepA"
                     /locus_tag="VF357_02540"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX7523053.1"
                     /translation="HAGVLPRQGEILRNLVDMRIENIRNFSIIAHIDHGKSTLADRLL
                     EITGTLSDREKVDQFLDKMDLERERGITIKAQTVRMHHRAEDGKEYILNLIDTPGHVD
                     FSYEVSRSLSACEGAILVVDASQGVEAQTLANVYMALDLNLEVVPVLNKIDLPNAETE
                     RVRQEIEDVIGLDTSGILAVSAKKGTGVPELLEKVIRVIPPPEGDPDAPTKALIIDSW
                     FDNYAGVVVLARVLDGAIRSGQRVTFMATGNSFEVQKVGVFSPHPEELQALGTGEVGF
                     VIANIKELTETKVGDTITDTRHPTARPLPGFKVVRPMVFAGVYPLASDQYPQLRTAID
                     KLRLNDSSFTAEPESSVALGFGFRCGFLGLLHMEIVQERLEREYDVQLITTAPTVEYR
                     AVKIDGTVLEVDSPARLPEPLELDHIEEPIIHATIHLPAEYLGNVLKLCEERRGVQRQ
                     MKFVSAARVILEYELPLNEIVLDFYDKLKTASRGYASMDYDFLRFQQSNLVRLDILLN
                     GDKVDALSLITHRDNSYPKGRDVTERLRKLIPRQMFEVVIQAAIGGKIIARESVKAIR
                     KNVIAKCYGGDISRKRKLLEKQKEGKKRMKQVGSVEIPQEAFLSILKVKE"
     gene            1916..2590
                     /gene="lepB"
                     /locus_tag="VF357_02545"
     CDS             1916..2590
                     /gene="lepB"
                     /locus_tag="VF357_02545"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7523054.1"
                     /translation="MMSEHGIRATGSKKGKVREYAEAFGVALVIALIVRTLFLQAFKI
                     PSGSMENTLLVGDHIFVNKFVYGYHVPYTKGRILAFTTPKRGDIVVFVFPEDPSKDFI
                     KRVIGTPGDVVEIRQKTVYINGAPLVEEYTRFADGKNSDGGVIRGRDNMPPVRVPAGK
                     LFMMGDNRDRSYDSRFWGFVDMDAVIGRALFLYFSIDWNRGIGWTDVGRYPELVRWDR
                     IGRVLH"
     gene            2784..3344
                     /gene="pyrR"
                     /locus_tag="VF357_02550"
     CDS             2784..3344
                     /gene="pyrR"
                     /locus_tag="VF357_02550"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX7523055.1"
                     /translation="MKKTGMKTLLDGKGVERVLSRLTHEIIEKNKGAGQIVLVGIASG
                     GIPLSQVIRGKIQVIEGLDVPTGFVDITLYRDDLARAGYQARMKRTEIPFSIDDKKVI
                     LVDDVLYTGRTIRAAMDALIDFGRPRNIQLAVLIDRGHRELPIRADFVGRNVPTSRSE
                     TVLVRVEGTPEEWTVLLKEDPSGKGE"
     gene            3348..4280
                     /locus_tag="VF357_02555"
     CDS             3348..4280
                     /locus_tag="VF357_02555"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX7523056.1"
                     /translation="MEWTRRHVLGLSDFHPEEMEFVIDTARSMEEVLTGDIKKVPALR
                     GKTVVNLFFEASTRTRTSFEIAGKRLSADVVNFNASTSSVTKGETLLDTARNIEAMKP
                     NIVVVRHSASGAPLFLSRHLSCSIINAGDGANEHPSQGLLDLYTIMKVKGKISGLKIA
                     IVGDILHSRVVRSDLHSLGRMGAKLWLCGPATLLPREMERTGAVVTSDIREAVRGADV
                     IIMLRIQLERQKTAYFPSLREYSRRFGLNRDVFSLAKDDAVIMHPGPINRGVELSDEL
                     ADCDRSLVLRQVESGVAVRMALLYLLAGGSHVAH"
     gene            4267..>5499
                     /locus_tag="VF357_02560"
     CDS             4267..>5499
                     /locus_tag="VF357_02560"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX7523057.1"
                     /translation="MLLIKGGRVIDPASGRDEIGHLVLEGGKVREFLAGDAPARFQGK
                     VISAEGMWVVPGLIDMHVHLRDPGYEWKEDISTGTRAAVAGGFTSVVCMANTNPVNDS
                     PEVTRHIVEKARREGCANVFPVAAVTRGLEGKEMADFSELADAGAVAFSDDGKPVENS
                     LLLRRAMEYVRPFGYRILSHAEDPVLAGGGAAHEGWIARKLGIPGIPSAAEEVAIARD
                     ILIARQTGGKLHIQHITTRMGVDLLRMARRAGIDVTGETAPQYFTLTDAALEGYDTNA
                     KMNPPLRGEDDRMAILEGIQDGTIDAIACDHAPHEAYLKRCEFAAAANGIIGLQTSLP
                     LSLSLLAGGKVAPARLVELLSVGPARILNLRGKGTLAPGADADVTVIDPDAEWEFALG
                     DVLSKSKNSPFFGWKMRGR"
BASE COUNT         1073 a         1704 c         1769 g          953 t
ORIGIN      
        1 tcacgctggc gtacttccga gacaaggtga aatcctccgg aacctggtag acatgcgaat
       61 agagaacatc cgcaattttt ccatcatcgc ccacatcgac cacgggaagt ccaccctggc
      121 cgaccggctc ctcgagatca ccggcaccct ttcggatcgg gaaaaggtcg accagttcct
      181 ggacaagatg gacctcgaac gggagcgcgg gatcacgatc aaggcccaga ccgtacggat
      241 gcaccaccgc gcggaggacg gaaaggaata catcctcaac ctgatcgaca cccccggcca
      301 cgtcgacttc tcctacgagg tgtcccgcag cctgagcgcc tgcgaagggg cgatcctcgt
      361 cgtggacgcg tcgcagggcg tcgaggcaca gacgctggcc aacgtctaca tggccctcga
      421 cctgaacctc gaggtcgtcc ccgtcctgaa caagatcgat cttccgaacg cggagaccga
      481 acgggtgcgg caggagatcg aagacgtcat cggcctcgac acctccggga tccttgcggt
      541 cagtgcgaag aaggggaccg gcgtcccgga acttctcgag aaggtgatcc gggtgatacc
      601 cccccccgag ggggatccgg acgcgccgac caaggcgctg atcatcgatt cctggttcga
      661 caactacgcc ggcgtggttg ttctcgcccg ggtgctcgac ggcgcgatcc ggtccggtca
      721 gcgggtcacc ttcatggcga cggggaattc gttcgaagtg cagaaggtcg gcgtcttctc
      781 tccccatccg gaggaactgc aggcgctggg gaccggggaa gtcggtttcg tgatcgccaa
      841 catcaaggaa ctgaccgaga cgaaggtggg cgacacgatc accgacacgc gccatcccac
      901 cgccaggccg ctcccgggat tcaaggtcgt ccggccgatg gtgttcgcgg gcgtttaccc
      961 gcttgcctcc gaccaatatc cccagctacg gacggcaatc gacaagctac ggctcaacga
     1021 ctcctcgttc acggcggagc cggagagctc cgtggcgctg ggattcggct tccggtgcgg
     1081 atttctcggg ctgctccaca tggagatcgt ccaggagcgg ctggagcgcg aatacgacgt
     1141 gcagctcatc accacggccc ccaccgtcga gtaccgcgcg gtgaagatcg acggcacggt
     1201 cctggaggtc gatagccccg cccggctccc ggaaccgttg gagctcgacc atatcgagga
     1261 accgatcatc cacgcgacga tccatcttcc ggcggaatac cttggaaacg tcctcaagct
     1321 ttgcgaagag cggcggggcg tgcagcggca gatgaagttc gtttcggcgg cccgggtcat
     1381 cctcgagtac gaactgccgc tgaacgagat cgttctcgac ttctacgaca agctcaagac
     1441 cgcttcccgc gggtacgcct cgatggatta cgattttctc cgatttcagc agtccaacct
     1501 ggtccggctg gacatcctgc tcaacgggga caaggtggac gccctttcgc tcatcacgca
     1561 tcgggacaac tcctacccga aggggcggga cgtcacggag cgcctgcgga aattgatccc
     1621 gcgccagatg ttcgaggtgg tcattcaggc ggcgatcggc ggcaagatca tcgcccggga
     1681 gtcggtcaag gcgatccgga agaacgtcat cgcgaaatgc tacggcggcg acatctcgag
     1741 aaagcggaag ctcctggaga agcagaagga agggaagaag cggatgaagc aggtcggctc
     1801 cgtcgagatc ccccaggaag cgttcctgtc catcctcaag gtgaaggaat gaccctcggt
     1861 tccgtgccta aatcgccgca tttatgaatg gaagtacaaa gggaggatgc gaaggatgat
     1921 gtcggaacat gggattcgcg caacaggttc gaagaagggg aaggtccgtg aatacgccga
     1981 ggcgttcggc gtcgccctgg tcatcgccct gatcgtccgc accctctttc tgcaggcgtt
     2041 caagatcccg tcgggaagca tggagaacac gctcctggtc ggggaccaca tcttcgtcaa
     2101 caagttcgtc tacgggtacc acgtgccgta cacgaaggga cggatcctcg ccttcaccac
     2161 gccgaaacgg ggtgacattg tcgtcttcgt ctttcccgag gacccgagca aggatttcat
     2221 caagcgtgtc atcgggactc cgggggacgt cgtggagatc cggcagaaga cggtctatat
     2281 caacggggcc cctcttgtgg aagagtacac gcgtttcgcg gacgggaaaa actccgacgg
     2341 tggtgtcatt cgcggcaggg acaacatgcc ccccgtgcgg gtgcccgccg gaaaactctt
     2401 catgatgggg gacaaccgcg accggtcgta cgactcccgc ttctgggggt tcgtcgacat
     2461 ggacgccgtc atcggcaggg cgctgttcct ctacttctcc atcgactgga atcgcggcat
     2521 cggatggacg gacgtgggac gctaccccga gctcgtccgc tgggatcgga tcggacgtgt
     2581 tttgcattag tgcccctcct tccctttgcc gttgactcgg gagggggatg agggtagtat
     2641 ctcaaatggc cttttaagct aatcccgtga ggttagcaaa ggatgaagat ttgccgatca
     2701 cgacattgcc tctccgcgac gacggagagg catttttttt tcccccgacg ccggcacacc
     2761 ggccgggaca cgaggcggac cccatgaaga aaaccggcat gaagaccctc ctggacggca
     2821 agggcgtgga gcgcgtactc tcccgcctga cccacgagat tatcgagaag aacaaggggg
     2881 cggggcaaat cgtcctcgtc ggaatcgcct cgggggggat ccccctttcc caggtgatcc
     2941 gcgggaagat ccaggtgatc gaagggctgg acgtccccac cggattcgtc gacatcaccc
     3001 tctaccggga cgatctcgcc cgcgcgggat accaggcgcg catgaagcgg acggagatcc
     3061 cgttctccat cgacgacaag aaggtgatcc tggtagacga cgttctctac acgggccgga
     3121 cgatccgggc ggccatggac gcgctgatcg acttcggtcg cccgcgaaac atccagctgg
     3181 cggtcctcat cgaccggggg caccgggagc tccccatccg tgccgacttc gtggggcgga
     3241 acgtccccac gtcccggtcg gagaccgtcc tggtgcgcgt cgaggggacg ccggaagaat
     3301 ggacggtcct tttgaaggaa gatccgtcag ggaaggggga ataggccatg gaatggactc
     3361 gccggcacgt cctcgggctc tccgacttcc acccggaaga gatggagttc gtcatcgata
     3421 ccgcgcggtc gatggaggag gtgctcacgg gcgacataaa gaaggtcccc gccctgcgcg
     3481 ggaagaccgt ggtcaacctc ttcttcgagg ccagcacgcg gacacgaacc tcgttcgaga
     3541 tcgccgggaa gcgcctctcc gccgacgtgg tgaacttcaa cgcgagcaca tcgagcgtca
     3601 cgaaggggga gacgctcctc gacacggcgc ggaacatcga ggcgatgaag ccgaacatcg
     3661 tcgttgtacg gcactccgcg tcgggcgcgc cccttttcct gtcccgccac ctctcctgct
     3721 cgatcatcaa cgcgggagac ggcgcgaacg aacatccttc ccagggcctg ctcgatctct
     3781 acacgatcat gaaggtgaaa gggaagatct ccggcctcaa gatcgccatc gtgggggata
     3841 tcctgcacag ccgggtcgtc cggtcggacc ttcactccct agggaggatg ggggcgaagc
     3901 tgtggctgtg cggaccggcg accctccttc cccgggagat ggagcggacc ggggcggtcg
     3961 tgacgagcga cattcgcgaa gcggtacggg gagccgatgt catcatcatg ctcaggatcc
     4021 agcttgagag gcaaaaaacc gcttattttc catcattaag ggagtattcc cgcaggtttg
     4081 gcttgaaccg tgacgtcttc tccctcgcga aggatgacgc ggtgatcatg catccgggac
     4141 cgatcaaccg tggagtggag ctgtccgacg aactggccga ctgtgatcgg tccctcgtgc
     4201 tgcggcaggt cgagtccgga gtggctgttc ggatggcgct tctctacctt ctcgctggag
     4261 gttcccatgt tgctcattaa gggggggagg gtgatcgacc cggcgtccgg tcgggacgaa
     4321 atcggtcacc tggtactgga gggcgggaag gtccgcgagt tcctggcggg cgatgccccc
     4381 gcgcgattcc aggggaaggt gatctccgcg gaggggatgt gggtcgtgcc gggcctgatc
     4441 gacatgcacg ttcacctgcg tgatccggga tacgagtgga aagaggacat ctccaccggg
     4501 acacgcgccg ccgtcgccgg cgggttcacc tcggtcgtct gcatggccaa cacgaatccg
     4561 gtcaacgatt ccccggaggt cacccggcac atcgtcgaga aagcgcgtcg ggaagggtgc
     4621 gccaacgtct ttcccgtggc cgcggtgacc cgcgggctcg aaggaaaaga gatggcggac
     4681 ttctctgaac tggccgacgc cggagcggtg gctttttcgg acgacgggaa accggtggag
     4741 aactccctcc tgcttcggcg cgcgatggag tacgtccgtc ccttcggata tcgcatcctc
     4801 tcccacgcgg aggatcccgt cctggccggg ggcggggccg cgcacgaggg atggatcgcc
     4861 cggaagctcg ggatcccggg gatcccctcc gccgcggagg aggtcgccat cgcccgggac
     4921 atcctgatcg cccggcagac cggggggaag ctgcacatcc agcatatcac cacccggatg
     4981 ggggtggacc tcctccggat ggcccgtcgc gccggtatcg acgtcacagg cgagacggct
     5041 cctcaatact tcaccctgac cgacgcggcg ctcgaggggt acgacacgaa cgcgaagatg
     5101 aacccgccgt tgcgcggcga ggacgaccgg atggcgattc tcgaagggat ccaggacggc
     5161 acgatcgacg cgatcgcctg cgaccacgcc ccgcacgagg cgtatctcaa gaggtgcgag
     5221 ttcgccgccg ccgccaacgg catcatcggc ctgcagacct ctcttccgct ctcgctttcc
     5281 ctgctggccg gaggaaaggt cgctccggcg cgtctcgtcg aattgctctc cgtcggaccc
     5341 gcgcggatcc tcaacctgcg cggcaaggga acgctcgccc ccggcgcgga cgccgacgtc
     5401 acggtgatcg accccgatgc ggaatgggaa tttgcgctcg gagacgtgct ctcgaagtcg
     5461 aagaacagtc ccttcttcgg gtggaagatg cgcggccgc
//
LOCUS       DASVJL010000129         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1802465, whole genome shotgun sequence.
ACCESSION   DASVJL010000129 DASVJL010000000
VERSION     DASVJL010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802465"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VF357_02565"
     CDS             <1..889
                     /locus_tag="VF357_02565"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX7523058.1"
                     /translation="DIDTVFMGDIMSVSKIGVLGAGQMGSGIAQVSLMSGLQVVLNDV
                     TEAVLARSRAGIGKGLDILVRKEKITTQEKERALNGLQTTTDPCDFASCDLVVEAATE
                     REELKLSLFRKLDEAVPAGRILATNTSSISITKIAAATNRPAQVIGMHFMNPVPLMKL
                     VEVIRGLQTSTETFDATMALARALGKEPVPANDFPGFIANRILMPMINEAVYALMEGV
                     GEAADIDAIMKMGANHPMGPLALADLIGLDTCLEVMNVLQQGLGDSKYRPCPLLRKHV
                     DAGYLGKKTGRGFYTYEKT"
     gene            900..1682
                     /locus_tag="VF357_02570"
     CDS             900..1682
                     /locus_tag="VF357_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7523059.1"
                     /translation="MNYENLLVDVSERIATVTFNRPKFLNALNPATVRELGAAMEELS
                     ARPDVGAVLLTGAGEKAFIAGADISVMKGFTTIEALDFSLLGQRVLAFIESMSQPVIG
                     VINGFALGGGCEVAMACDLLIAADTARFGQPEVNLGIIPGYGGTQRLPRLVGRNLAKE
                     LVLTGEMISAQRAYEIGLVNKVVPAAELMGAARELAKKILSRGPVAVRTAKMAMNRGL
                     DLDLGNASALEASLFAAGFSTADRVEGIAAFLEKRKANFTGK"
     gene            1729..>2558
                     /locus_tag="VF357_02575"
     CDS             1729..>2558
                     /locus_tag="VF357_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7523060.1"
                     /translation="MVFDLTEEQRMIQDTAREFARREVLPKAAELDENSRFPEELIRQ
                     MAELGFMGIAVPEEYGGAGMDNVCYAIAMEEISRACASTGVIMSVNNSLACDPILKFG
                     SEEIKQEYLVPMASGKKLGCFGLTEPGAGSDAGSQKTTAVRDGDFYVVNGTKNFITNA
                     PQADTCVLFTMTDKAKTHKGITAFVVDMKWEGISLGKHEKKMGIKGSATSSIIFEEVR
                     VPAKNRLGNEGDGFKVAMNTLDGGRIGIASQAIGIARASFEDALSYAKDRKQFGQAIC
                     "
BASE COUNT          511 a          774 c          864 g          409 t
ORIGIN      
        1 tgatatcgat acggttttca tgggggatat aatgagcgtt tcgaagatcg gcgtgctcgg
       61 agccggccag atgggaagcg ggatcgcgca ggtctccctg atgagcggct tgcaggtcgt
      121 cctgaacgat gtgaccgagg cggtccttgc ccggtcccgc gcgggaatcg ggaaaggtct
      181 tgacatcctc gtccgcaagg agaagatcac gacgcaggag aaggaaaggg cgctcaacgg
      241 cctccagacg acgaccgatc cctgcgactt cgcctcctgc gatctcgtcg tggaagcggc
      301 cacggagcgg gaagagctga agctctccct tttccggaag ctcgacgagg cggtccccgc
      361 gggtcggatc ctcgcgacga acacctcctc catctcgatc acgaagatcg ccgccgcgac
      421 gaatcgtccg gcgcaggtga tcgggatgca cttcatgaat ccggttcccc tcatgaaact
      481 cgtcgaggtg atccgggggc tgcagacgtc gacggaaacg ttcgacgcga cgatggcgct
      541 cgcccgcgcg ctcgggaagg agccggtccc cgcgaacgac tttccggggt tcatcgcgaa
      601 ccggatcctc atgccgatga tcaacgaggc ggtctacgcc ctgatggaag gtgtggggga
      661 ggccgccgac atcgacgcga tcatgaagat gggggcgaac cacccgatgg ggccgctggc
      721 gctggcggac ctcatcggac tcgacacctg cctcgaggtg atgaacgtgc tgcagcaggg
      781 attgggagac tcgaagtacc gcccgtgccc gctcctgcgc aagcatgtcg acgccggcta
      841 cctcggaaag aaaacgggcc gcggcttcta cacgtacgaa aagacctgag gaggagccga
      901 tgaattacga aaacctgctg gtcgacgttt cggaacggat cgcgacggtc accttcaatc
      961 gcccgaaatt cctgaacgct ttgaaccccg cgacggttcg ggagctcggc gccgccatgg
     1021 aagagctctc ggcgcggccg gacgtgggag cggtccttct gaccggcgca ggggagaagg
     1081 cgttcatcgc gggggccgac atctcagtga tgaaagggtt tacgacgatc gaggcgcttg
     1141 atttctccct cctcggccag cgagtcctcg ccttcatcga gtcgatgtcc caaccggtca
     1201 tcggcgtgat caacggcttc gcgctgggcg gggggtgcga agtggcaatg gcgtgcgacc
     1261 tgctgatcgc cgcggacacg gcccggttcg gccagcctga ggtgaatctc gggatcatcc
     1321 cgggatacgg gggaacgcag cgcctgccgc gtctggtcgg gcggaacctc gcgaaggagc
     1381 tggtcctcac cggggagatg atctccgcgc agcgggcgta cgagatcggc ctggtgaaca
     1441 aggtcgtccc ggcggcggaa ctgatgggtg cggcgaggga gctcgcgaaa aagatcctct
     1501 cccggggacc ggtcgccgtc cgcaccgcaa agatggcgat gaaccggggg ctcgaccttg
     1561 acctgggcaa cgcaagcgcg ctcgaggcga gcctgttcgc cgccggtttc tccaccgccg
     1621 acagggtgga aggaatcgcc gcgttcctcg aaaaacggaa ggcgaatttc accgggaagt
     1681 gatccctggg gaagtcgccc cactttcaac gacaggggag acgaaacgat ggtgttcgac
     1741 ctgaccgaag agcagcggat gatccaggat acggcgaggg agttcgcccg gagagaggtc
     1801 ctgccgaagg cggccgagct ggacgagaac agccgcttcc ccgaggagct gatccgccag
     1861 atggccgagc tcggcttcat ggggatcgcc gttcccgagg agtacggcgg cgccggaatg
     1921 gacaacgtct gctacgcgat cgccatggag gagatctccc gcgcgtgcgc ttccaccggc
     1981 gtcatcatgt cggtcaacaa ttcgctcgcg tgcgacccga tcctcaagtt cggctccgag
     2041 gagatcaagc aggaatacct cgtgccgatg gcttccggga agaagctcgg ctgcttcgga
     2101 ctcacggaac ccggcgccgg ctccgacgcc ggttcccaga agaccaccgc ggtccgggac
     2161 ggcgatttct acgtcgtcaa cggcaccaag aacttcatca cgaacgcgcc gcaggcggac
     2221 acctgcgtcc tcttcacgat gaccgacaag gcaaaaacgc acaaggggat caccgcgttc
     2281 gtcgtcgaca tgaagtggga gggaatctcg ctcgggaagc acgagaagaa gatggggatc
     2341 aaggggtccg ccacctcttc gatcatcttc gaggaggtcc gcgtcccggc gaaaaaccgg
     2401 ctggggaacg agggggacgg gttcaaggtc gccatgaaca ccctcgacgg agggcggatc
     2461 gggatcgcct cccaggcgat cgggatcgct cgcgcctcgt tcgaagacgc cctctcctac
     2521 gcgaaggacc ggaagcagtt cggccaggcg atctgcga
//
LOCUS       DASVJL010000130         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1427618, whole genome shotgun sequence.
ACCESSION   DASVJL010000130 DASVJL010000000
VERSION     DASVJL010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427618"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VF357_02580"
     CDS             <1..340
                     /locus_tag="VF357_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523061.1"
                     /translation="LSPSIQEQNRTLIVEAEVANRDGRLRPGSFARAEIVVEADRNAV
                     MVPASTVVTFAGLEKVFVVKDGKAVEKRIRTGRRSGDRVEILDGVAAGEQVVADPGNL
                     VGGMPVTVTR"
     gene            348..>2207
                     /locus_tag="VF357_02585"
     CDS             348..>2207
                     /locus_tag="VF357_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7523062.1"
                     /translation="MQKLAEICIRRPIFAAMLILALVVVGGTSYLRLGVDRFPSVDLP
                     TVSIRTQLPGASAEEVETGISKRIEEVVNTVEGIDELRSISGPGASVVIVTFNLNRDI
                     DTAAQDVRDRVATILKDLPKDATPPLVSKFDNDQQPVLTVALAGDRPLRELTELADKV
                     VKIRLERSAGVGEVQIVGGLERAVNVWVEADRLAAFGIPITDVRDAIVRQNADAAGGN
                     VTRGAQESTLRTIGRLADPRAFDDLVVATIGGAPVRIRDIGRTEDGTREQRSTARLNG
                     VPTVVLEVRRQSGANTVAVIEGAKRSLAAAAGELPAGVGLTVVQDQSLYIYSALHEIN
                     VHLILGSILASLVVLAFMRSWRSTVIAAVAIPASVVSTFGMMWVLHFTLNSVTMLALV
                     LMVGIVIDDAIVVLENIFRFVEEKGMPPFEAARAATADIGLAVMATTFSLVVIFIPVS
                     FMSSISGRFLYQFGITAAVAVLVSLLVSFTLTPMMSARLLGGDAARTQGAPHSRGGFY
                     RRIDAGYEGLLALALRHRAAVALISVAVMLSVIPLYGLIRQDYLPANADEGEFNVSVT
                     APQGTSLAGMDGIMNDIVAEIRAVPGVRMVLSTAGGGWIGGVSEGRAYIRLAPH"
BASE COUNT          328 a          740 c          768 g          371 t
ORIGIN      
        1 cctgagcccg tccatccagg agcagaaccg cacgctgatc gtcgaagccg aagtggcgaa
       61 ccgggacggg aggcttcgcc ccggctcgtt cgcgcgcgcc gagatcgtgg tcgaggccga
      121 ccgcaacgcc gtcatggttc cggcctcgac ggtggtgacc ttcgccggcc tcgagaaggt
      181 gttcgtcgtc aaggacggga aggccgtgga aaagcggatc cggaccggcc gccgctccgg
      241 cgatcgggtg gagattctcg atggggtcgc cgccggcgag caggtcgtgg ccgaccccgg
      301 aaacctcgtc ggtgggatgc ctgtcaccgt gacccggtag ctcgttcttg caaaagctcg
      361 ccgaaatctg catccgccgc cccatcttcg cggcgatgct tatcctcgcg ctggtcgtcg
      421 tgggcggtac cagctatctc cgcctcggcg tcgaccggtt cccctcggtg gaccttccca
      481 ccgtctcgat ccgcacccag ctccccggcg cctccgcgga agaggtagag accgggatct
      541 ccaagcggat cgaggaagtg gtcaacaccg tcgaggggat cgacgagctg cggtcgatct
      601 cggggccggg cgcctccgtc gtgatcgtca ccttcaacct caaccgggac atcgacacgg
      661 cggcgcagga cgtgcgggat cgcgtcgcca ccatcctcaa agacctgccg aaggacgcga
      721 cgccgcccct ggtttcgaag ttcgacaacg accagcagcc ggtcctgacg gtggccctgg
      781 cgggcgaccg gcccctccgc gagctgaccg agctggcgga caaggtggtg aagatccgcc
      841 tggagcggtc ggcgggcgtc ggggaggtgc agatcgtcgg cggattggag cgcgcggtca
      901 acgtttgggt ggaggcggac cggctggcag ccttcgggat ccccatcacc gacgtgcgcg
      961 acgcgatcgt ccggcagaat gccgacgccg ccggaggcaa cgtgacccgg ggcgcccagg
     1021 aatcgacgct ccggacgatc ggccgtctgg ccgaccctcg cgccttcgac gacctggtgg
     1081 tcgccacgat cggcggcgcg cccgttcgca tccgggacat cggccgaacg gaggacggga
     1141 ccagggaaca gcgctccacc gcccggttga acggcgtccc cacggtggtc cttgaggtgc
     1201 gacgccagtc gggggccaac accgtcgccg tcatcgaggg cgcgaagcgc agcctcgccg
     1261 ctgccgccgg ggaactgccc gccggggtcg gcctcacggt ggtccaggac cagtccctgt
     1321 atatctactc cgctctgcac gagatcaacg tgcacctgat cctcgggagc atcctggcct
     1381 ccctggtcgt cctggccttc atgcgaagct ggcgatcgac cgtgatcgcc gcggtcgcca
     1441 tcccggcgtc ggtggtctcc accttcggga tgatgtgggt tctccacttc acgttgaaca
     1501 gcgtgacgat gctcgccctt gtcctgatgg tcgggatcgt gatcgacgac gcgatcgtcg
     1561 tcctcgagaa catcttccgg ttcgtggagg agaaggggat gccgccgttc gaggccgcga
     1621 gggccgccac ggccgacatc gggctggccg tgatggcgac gaccttttcg ctggtggtca
     1681 tcttcatccc ggtgtcgttc atgtcgagca tctccggccg cttcctttac cagttcggca
     1741 tcaccgcggc cgtggcggtc ctcgtgagcc tgctcgtctc cttcaccctg acccccatga
     1801 tgagcgcgcg gctgttgggg ggcgatgcgg cgcggacaca gggcgccccg cactcccggg
     1861 gtggattcta tcggcggatc gatgccggat acgaagggtt gcttgccctc gccctccgcc
     1921 accgggcggc agtggcgctg atttcggtgg ccgtcatgct ttcggtcatc cctctgtacg
     1981 gcctgattcg ccaggattac ctcccggcca acgcggacga gggggagttc aacgtgagcg
     2041 tgaccgcccc gcagggcacg agcctggccg gcatggacgg gatcatgaac gacatcgtcg
     2101 ccgagatccg ggccgtgccg ggggtgagga tggtgctttc gaccgccggg ggcgggtgga
     2161 tcggcggggt gagcgagggg agggcgtaca tccggcttgc cccgcac
//
LOCUS       DASVJL010000131         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1521189, whole genome shotgun sequence.
ACCESSION   DASVJL010000131 DASVJL010000000
VERSION     DASVJL010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521189"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /gene="leuB"
                     /locus_tag="VF357_02590"
     CDS             complement(<1..756)
                     /gene="leuB"
                     /locus_tag="VF357_02590"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX7523063.1"
                     /translation="MAAKKICVFPGDGIGPEVMREALAVLRTVEKVSGGHRFETEEAL
                     LGGASYDAHGTPMTPGALALAKASDALLLGAVGGPKWDVLPFDLRPERGLLGLRRELG
                     LFANLRPAVVFAPLLGASPLRRELVEGIDLLVVRELTGGIYFGEPRGESVINGVETGI
                     NTEIYTRPEVERVARVAFDLARKRTRKVTSVDKSNVLESSVLWRRVVSEVGAKEYPEV
                     ALSHMLVDNCAMQLIRNPRQFDVVVTTNLFGDIL"
     gene            complement(756..1253)
                     /gene="leuD"
                     /locus_tag="VF357_02595"
     CDS             complement(756..1253)
                     /gene="leuD"
                     /locus_tag="VF357_02595"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX7523064.1"
                     /translation="MELSGKAWKYGDDVDTDVIIPARYLNTSDPAELASHCMEDIDAS
                     FASKVAPGDFIVAGKNFGCGSSREHAPIAIKASGASAVIARSFARIFYRNAFNMGLPI
                     FEAPDAVDEIEAGDRLAVDMERGSLRNETRGKEYRFTPIPPFMRELLAAGGLLNYIVK
                     RKKGT"
     gene            complement(1265..>1910)
                     /locus_tag="VF357_02600"
     CDS             complement(1265..>1910)
                     /locus_tag="VF357_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX7523065.1"
                     /translation="SMAWRFTMANMAIEAGAKNGIFPFDAATKAYADGRAKRKYEVVT
                     ADPDARYADEISVDLSALEPVVAFPHLPENTRPLSKVGNVPIDQVVVGSCTNGRIEDL
                     RSAAAVIRGRKVHDGVRMIIFPATQEIYLQAMREGLMEAFVTAGAAFSTPTCGPCLGG
                     HMGILAKGERAISTTNRNFVGRMGHPESEVYLSNPAVAAASAVLGRIGGPDELP"
BASE COUNT          300 a          699 c          598 g          313 t
ORIGIN      
        1 gaggatgtcg ccgaagaggt tcgtcgtcac caccacgtcg aactgccgcg ggttgcggat
       61 gagctgcatc gcgcagttgt cgaccagcat gtgggagagg gccacttcgg ggtactcctt
      121 cgcgcccacc tcggacacca ccctgcgcca cagcaccgag gattcgagga cgttggactt
      181 gtccaccgaa gtgactttcc gggtccgctt gcgcgccagg tcgaaggcga cccgggcgac
      241 ccgctcgacc tcgggccggg tatagatctc cgtgttgatt cccgtctcca cgccgttgat
      301 cacggattcg ccgcgcggct cgccgaaata gatcccgccc gtcagctcgc gaacgaccag
      361 gaggtcgatc ccctcgacca gttcccgccg cagcggcgag gcgccgagga ggggcgcgaa
      421 gaccaccgcg ggacgcaggt tggcgaacaa ccccagctcc ctgcgcagtc ccagcagtcc
      481 ccgctccggg cgcaggtcga aggggaggac gtcccacttc ggaccgccca ccgcccccaa
      541 cagcagcgcg tcggatgcct ttgccaaggc cagggcgccc ggagtcatcg gggtcccgtg
      601 ggcgtcgtag gaagcgccgc ccaggagcgc ctcctccgtc tcgaaccgat gccccccgga
      661 aaccttctcc accgtccgca ggacggccaa ggcctcccgc atcacctccg gaccgatccc
      721 gtcgcccgga aaaacgcaga tcttcttcgc cgccatcagg tccccttctt tcttttcacg
      781 atgtagttca gcaaaccccc ggcggccaac agctcccgca tgaacggggg gatcggggtg
      841 aaacgatact ccttccccct tgtctcgttc cgcagggagc cccgttccat gtccacggcg
      901 aggcggtctc cggcctcgat ctcgtcgacc gcgtccgggg cctcgaagat cggcagcccc
      961 atgttgaagg cgttccggta gaagatccgg gcgaacgagc gggcgatgac cgccgacgcg
     1021 ccggacgcct tgatcgcgat cggggcgtgc tcccgcgagg agccgcaccc gaagttcttc
     1081 cccgcgacga tgaagtcgcc cggagccacc ttcgacgcga acgaggcgtc gatgtcctcc
     1141 atgcaatgcg aggcgagctc cgccgggtcg gaggtgttca ggtaccgcgc ggggatgatg
     1201 acgtccgtgt cgacgtcgtc cccgtacttc cacgccttgc cgctcagttc catgtcatgc
     1261 gctcctacgg aagttcgtcc gggccgccga tgcggccgag gaccgcggaa gccgcggcca
     1321 ccgcggggtt ggagaggtac acctcgctct cggggtgccc catccggccg acgaagttcc
     1381 ggttcgtcgt cgagatcgcc cgctctccct tcgccaggat ccccatgtgg ccgcccaggc
     1441 acggcccgca ggtgggggtg gagaaggccg cccccgccgt gacgaacgcc tccatcaggc
     1501 cctcccgcat cgcctgaagg tagatctcct gggtggcggg aaagatgatc atccgcacgc
     1561 cgtcgtggac ctttcgcccc cggatcaccg ccgcggcgct gcgcagatcc tcgatccgtc
     1621 cgttggtgca cgaccccacc acgacctggt cgatcgggac gttccccacc tttgagagcg
     1681 gcctcgtgtt ctccggcagg tgcgggaacg cgaccaccgg ctcgagcgcg gacaggtcga
     1741 cggagatctc gtccgcgtag cgcgcgtccg gatcggccgt cacgacctcg tacttccgct
     1801 tcgcccttcc gtcggcgtac gccttcgtcg ccgcgtcgaa cgggaagatc ccgtttttcg
     1861 cccccgcctc gatcgccatg ttcgccatgg tgaagcgcca cgccatcgac
//
LOCUS       DASVJL010000132         3540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_301534, whole genome shotgun sequence.
ACCESSION   DASVJL010000132 DASVJL010000000
VERSION     DASVJL010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301534"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..2158)
                     /locus_tag="VF357_02605"
     CDS             complement(29..2158)
                     /locus_tag="VF357_02605"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HEX7523066.1"
                     /translation="MPGSAPSRRALLLCLHNHQPVGNFDSVIEGAARDAYLPFLQTLA
                     DFPSVKVTIHFSGFLLRWLAERSPDTFSLLKLLSDRGQAEVLGGGMYEPILALLPERD
                     RLGQIEALAAEVTRFFGKAPEGIWLAERVWEPDLPATLFAAGVKYLPLDDYHFVRAGL
                     SPGELDGIYLTEYNGAPVRVFPGSEQLRYMIPFGDVGETLREIERLTSRAVPYPAAIF
                     ADDGEKFGVWPGTHRSVYGEGWLRRFFGGVAERADWLTTMTFGEYASVAPPRGTVYLP
                     TCSYAEMGEWTLPPGGAARFGDLLYELRSGKMAEIKPFLQGGYFRNFLRKYDESNQLH
                     KRMLWVSERVAAARRERPVRGRAACDFLYRAQSNDVYWHGVFGGLYLNHLREAAYANL
                     LRAEAASDAVLHEEKERWTEAERGDIDRDGGEELLLKTSGLTLLAHAHDGGAVTEISL
                     PRRAVALGHVLTRREEGYHEKFRRAVGSFDGSTSIHDAMVLKDPSVLSALGVDPWQRA
                     SFREAYYGDGDLPEAILSGAAAPRCFTAGREASVTVLRRGSRLTAEFAVPFGGEGIGL
                     HLVKALVLRGRGERFEARFRLENRGAETVSGWFCSEWNLNMLSGNGPDRYYEGMGGAR
                     ALSSSGVAKGGRGFRVVDAWRNVAAGAALDRECVVLREPVETASLSESGAEKIHQGVC
                     LRILFPVRLEPGNHERYSILWSFKSVD"
     gene            complement(2161..2574)
                     /gene="mce"
                     /locus_tag="VF357_02610"
     CDS             complement(2161..2574)
                     /gene="mce"
                     /locus_tag="VF357_02610"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX7523067.1"
                     /translation="MIRRIQHIGIAVRSLQEAIPFYRDVLGLELVGTEEVTDQKIRAA
                     VFRVGESTIEVIESTAPGGPVGKFIERNGEGIHHLCFEVDDAAAALSHAKGKGVRLID
                     ETPRPGVHGMRIGFLHPKATFGVLTEFAQAGDEGH"
     gene            complement(2598..>3540)
                     /locus_tag="VF357_02615"
     CDS             complement(2598..>3540)
                     /locus_tag="VF357_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX7523068.1"
                     /translation="INGFHDTANAIATCISTRALSIRSAILMAAVLNFVGAFVSTQVA
                     TTIGKGIVDPGDVTQIVVLSALAGAIFWDLLTWHYGIPASSSHAIIGGLIGAVVAARG
                     FHPLQWGGISKILIAIVFSPIAGTLFAFLMMVAIYWAFRNYHPSPLNRAFRKLQVFSA
                     AFMAFSHGSNDAQKSMGVITLALVSYGAMPVFHIPVWVIASCATAMALGTALGGWRII
                     KTVGSDFVELQPVHGFCAETSSSAVILTATAMGIPISTTHVITSAILGVGLTQGRKKV
                     NWKVGIRIVWAWILTIPASAAAGYFAFRVLSPFLVKL"
BASE COUNT          624 a         1235 c         1153 g          528 t
ORIGIN      
        1 tggtcccaac ataccgtttt atgattcgtc aatcaacgga tttgaacgac caaagaattg
       61 aatatcgttc atggtttccg ggctccagcc ggacggggaa gaggatccgc aggcagacgc
      121 cctggtgaat cttctccgca cccgactcgg aaagggatgc cgtttccacc ggctcccgca
      181 gcacgacgca ctcccggtcg agggccgccc ctgcggccac gtttcgccag gcatccacga
      241 cgcggaaacc gcgccccccc ttcgcgaccc cggaagagga aagcgcgcgc gccccgccca
      301 ttccttcgta ataccggtcc ggcccgttcc ccgacagcat gttcaggttc cactccgagc
      361 agaaccaccc cgacaccgtc tccgcgcccc ggttttcgag ccggaaacgc gcctcgaatc
      421 gctccccgcg cccgcgaaga accagcgcct tcaccaggtg gagaccgatc ccttcccctc
      481 cgaaagggac cgcgaactct gccgtgaggc ggctcccccg ccgcaggacc gtgacggacg
      541 cctcccggcc cgcggtgaaa cagcgggggg cggccgcccc ggaaaggatc gcctcgggga
      601 gatccccgtc gccgtaatac gcctcccgga aggaagcccg ctgccacgga tcgaccccga
      661 gggcggacag gaccgagggg tctttcaaga ccatcgcgtc gtgaatgctc gtggacccgt
      721 cgaacgaccc gaccgcccgg cgaaacttct cgtggtaccc ttcctcccgc cgcgtcagca
      781 cgtgcccgag cgccaccgcc cgccggggga gggagatctc cgtcaccgcc ccgccgtcat
      841 gggcgtgggc gagcagggtc agccccgacg tcttcaggag cagttcctcc ccgccgtccc
      901 ggtcgatgtc tccgcgctcc gcttccgtcc accgctcctt ctcctcgtgc aggaccgcgt
      961 cggacgccgc ctcggcgcgg aggaggttcg cgtacgccgc ctcgcgcagg tggttgaggt
     1021 agagcccccc gaagacgccg tgccagtaga cgtcgttgct ctgcgccctg tagaggaagt
     1081 cgcatgccgc gcgcccgcgg accggccgct cccgccgcgc cgccgcgacg cgctcgctca
     1141 cccacagcat ccgcttgtgg agctggttcg actcgtcgta cttgcgcagg aaattccgga
     1201 aatatccccc ctgcaggaac ggcttgatct cggccatctt tcccgagcgg agctcataaa
     1261 gcagatcgcc gaacctcgcg gccccgcccg gcggcaaggt ccactcgccc atctcggcgt
     1321 aggagcaggt gggcaggtag accgttccac gcgggggcgc gacggaggcg tactccccga
     1381 acgtcatcgt cgttagccaa tcggcgcgct ccgcgacccc cccgaaaaag cggcgaagcc
     1441 atccttcgcc gtagacactg cggtgcgtcc cgggccagac gccgaatttt tcgccgtcgt
     1501 cggcgaagat cgccgccggg tacggcacgg cacgggaagt cagccgctcg atctcccgca
     1561 acgtttcccc cacgtccccg aaggggatca tgtaccggag ctgctcgctc ccggggaaga
     1621 cgcgaacggg ggcgccgttg tattcggtga ggtagatgcc gtccagctcc ccgggcgaaa
     1681 ggcccgcccg cacgaagtgg tagtcgtcca acggaaggta cttcaccccc gcggcgaaaa
     1741 gcgtggccgg caggtccggc tcccacacgc gctcggcgag ccagatcccc tcgggtgcct
     1801 tcccgaagaa ccgcgtcacc tcggccgcga gcgcctcgat ctgtcccaag cggtcccgtt
     1861 ccggcagcag cgcgaggatc ggctcgtaca tccccccccc cagcacctcg gcctggccgc
     1921 ggtcggacag gagcttcagg agcgaaaacg tatcaggaga ccgttcggcc agccaccgga
     1981 gcaggaagcc ggagaagtgg atcgtgacct tgaccgacgg aaaatcggcg agggtctgga
     2041 ggaacggcag gtacgcgtcg cgggccgcgc cctcgatcac ggagtcgaag ttccccaccg
     2101 gctggtgatt gtggaggcac aggaggagag cgcgccggga cggcgcggag cccggcatgc
     2161 tcaatgtccc tcgtcccccg cctgcgcgaa ctccgtcagc acgccgaagg tcgccttcgg
     2221 gtggaggaag ccgatccgca tcccgtggac ccccgggcgg ggcgtctcgt cgataagccg
     2281 gacccccttc ccctttgcgt gcgacagggc ggcggcggcg tcgtcgacct cgaagcaaag
     2341 gtggtggatc ccctcgccgt tcctctcgat gaactttccc accgggccgc cgggggcggt
     2401 cgattcgatc acctcgatcg tgctctcgcc cacgcggaag acggcggcgc ggatcttctg
     2461 gtccgtcacc tcttcggtcc cgaccagctc cagcccgagg acgtcccggt aaaaggggat
     2521 cgcctcctgg aggctgcgga cggcgatacc gatatgctgg atccggcgga tcatggaaga
     2581 cctccctttc ttacagttta cagtttgacg aggaaagggg agaggacgcg gaaggcgaaa
     2641 tatccggcgg cggcggaggc ggggatcgtc aggatccacg cccagacgat ccggatcccc
     2701 accttccagt tcaccttctt ccgcccctgg gtaagcccca ccccgaggat cgcggaggtg
     2761 atgacgtggg tggtgctgat cgggatcccc atcgcggtcg cggtgaggat gacggccgac
     2821 gacgacgtct ccgcgcagaa gccgtggacc ggctgcagct ccacgaagtc gctcccaacg
     2881 gttttgatga tgcgccaccc gcccaacgcg gtcccgagcg ccatcgccgt ggcgcaggag
     2941 gcgatcaccc acaccgggat gtggaagacc ggcatcgccc cgtaggagac gagggcgagc
     3001 gtgatcacgc ccatcgactt ctgggcgtcg ttcgacccat gggagaaggc catgaaggcg
     3061 gccgagaaga cctgaagctt ccggaaggcg cggttgagcg gcgagggatg atagttccgg
     3121 aaggcccagt agatggcgac catcatgaga aacgcgaaaa gcgttcccgc gatgggggag
     3181 aagacgatgg cgatcaggat cttcgagatc ccgccccact ggagcgggtg gaatccccgc
     3241 gcggcgacca ccgccccgat gagccccccg atgatcgcgt gggaggaaga agccgggatc
     3301 ccgtagtgcc aggtgagcag gtcccagaag atcgccccgg ccagggccga caggaccacg
     3361 atctgggtga cgtctcccgg gtcgacgatc cccttgccga tcgtcgtcgc cacctgggtg
     3421 gagacgaagg cgcccacgaa gttgagcacc gccgccatca ggatggcgct gcggatggag
     3481 agggcgcggg tggagatgca cgtcgcgatg gcgttcgccg tgtcgtggaa gccgttgatg
//
LOCUS       DASVJL010000133         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1146216, whole genome shotgun sequence.
ACCESSION   DASVJL010000133 DASVJL010000000
VERSION     DASVJL010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146216"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1936
                     /gene="glgP"
                     /locus_tag="VF357_02620"
     CDS             <1..1936
                     /gene="glgP"
                     /locus_tag="VF357_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX7523069.1"
                     /translation="DVGRTPLYLLDSNIKANSAWSREITSTLYGGDRDMRIRQEILLG
                     VGGVRALKALGIQATVYHMNEGHSAFLIVERIRDLMASHGLTFAQAREVVLATGVFTT
                     HTPVPAGNERFDPDLLRKYLDPKIRPLGIPWEEFLALGQFGNPQSMEFGMTVFALRSS
                     AFANGVAKLHAETSRAMWKGLWPELPEAEVPIRAITNGIHTRSWLSHEMAELYTRYFG
                     PRFLEKPADHAVWERMEAIPPVELWRVHQSRRERLVFFARKRLKNQLRRQGAGMALQR
                     AAEEALNPEALTIGFCRRFATYKRANLLFRQPERLIRLLTNPDRPVQILFAGKAHPQD
                     LPAKEIIRSVIHFASDPRVRDRLVFLEDYDINVARYMVQGVDVWLNNPRRPLEASGTS
                     GMKAAANGALNVSILDGWWDEGYSPDLGWAIGSGEMYGDQEEQDRVECEALYSLLENE
                     IVPLFHDRDRGGLPRAWIAMMKASIRKLGAYFNTHRMVQEYTETSYLPAHRAGSRLSA
                     NDHSAARELTAWRERTTSAWPGITIRVEETRKHKEMRVGGTVGVAVRVRLGGLTPAEV
                     AVEIRYGFYDAAGQVGMGTILSARHDGRDGDEEIYRVEIPCAGSGRYGFAARVLPRHP
                     DLANALTPLRMTWEEAVPGA"
     gene            complement(1890..2645)
                     /locus_tag="VF357_02625"
     CDS             complement(1890..2645)
                     /locus_tag="VF357_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX7523070.1"
                     /translation="MTPALISVIIPALDEEASIARAIRSCREAGPCEVIVVDGGSRDR
                     TVEIARGRADAVISAPRGRATQMNAGAAVAGGEVLLFLHADTLLPGGSVPAVLGALQD
                     PAVIGGAFGVRLAASHGAGRYVRATLGITGRMIGARAFVSRSYSGDQAIFVRAEAFRT
                     VGGYPEIPLMEDVELSRRMRRTGKTVVLPLRVETSGQRWEAWGPLRTVLFMWRLRIGY
                     LLGRTPSRCAEAYRRGPALKRREPPPPKSSAGG"
     gene            complement(2982..>3406)
                     /locus_tag="VF357_02630"
     CDS             complement(2982..>3406)
                     /locus_tag="VF357_02630"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7523071.1"
                     /translation="GASVSFYLGKYFLHGVARGFLETRVPWLDRKAADEGFSVIFYLR
                     IFWFPFIVLNYAAGATRIRFRDYLLGTVLGLLPPVFIFTYFVGAMRDVLATYRRPADL
                     LTFDLLFPVLLLVFSFFLPTLLKRLRKGREFLPPGGQP"
BASE COUNT          591 a         1146 c         1206 g          463 t
ORIGIN      
        1 ggacgtcggc cgcacccccc tgtacctgct cgacagcaac atcaaggcga actccgcgtg
       61 gtcccgcgag atcacctcca ccctgtacgg gggggaccgg gacatgcgca tccggcagga
      121 gatcctgctc ggcgtcggcg gtgtgcgggc gctgaaggcc ctcggaatcc aggcgaccgt
      181 gtaccacatg aacgaggggc actccgcctt cctcatcgtc gaacggatcc gtgacttgat
      241 ggcgtcgcac ggcctcactt tcgcgcaggc gcgggaagtg gtgctcgcca ccggcgtctt
      301 caccacccac acccccgtcc ccgcggggaa cgagcggttc gatccggacc tgctccggaa
      361 atacctcgat ccgaagatcc ggccgctggg gatcccgtgg gaggagttcc tcgccctcgg
      421 ccagttcggc aatccacaat cgatggagtt cgggatgacg gtgttcgccc tccggtcgtc
      481 ggcgttcgcg aacggcgtgg caaagctcca cgccgagacc tcccgggcga tgtggaaagg
      541 tctgtggccg gagctccccg aagcggaagt gccgatccgc gcgatcacca acgggatcca
      601 cacccgttcc tggctcagcc acgagatggc ggaactgtac acgcggtact tcgggccgcg
      661 gttcctcgag aaacccgcgg accacgcggt gtgggagcgg atggaggcga tcccccccgt
      721 agagctctgg cgggtacacc agtcccgcag ggagcggctc gtcttcttcg cccgcaagcg
      781 gctgaaaaac cagctgcgcc gccaaggtgc cgggatggcg ctccagcggg cggcggagga
      841 ggcgctgaac cccgaggcgc tgacgatcgg cttctgccgg aggttcgcca cgtacaaacg
      901 ggccaacctg ctcttccggc agcccgaacg tttgatccgg ctcctgacga acccggaccg
      961 gccggtgcag atcctcttcg ccggaaaggc gcacccgcag gacctcccgg cgaaggagat
     1021 catccggtcg gtcatccatt tcgcgtcgga cccgcgggtc cgcgaccggc tggtcttcct
     1081 cgaggattac gacatcaacg ttgcgaggta catggtgcag ggggtcgacg tatggttgaa
     1141 caacccgcgg cgccccctgg aagcgtcggg gacgagcggg atgaaggcgg cggccaacgg
     1201 cgcgctgaac gtgtcgatcc tcgacgggtg gtgggacgag gggtactccc ccgacctcgg
     1261 gtgggcgatc gggagcggag agatgtacgg cgaccaggag gagcaagacc gtgtggagtg
     1321 cgaggccctc tacagcctcc tcgagaacga gatcgtcccc ctgttccacg atcgggaccg
     1381 cggcgggctg ccgcgggcct ggatcgcgat gatgaaggcg tcgatccgga agctgggcgc
     1441 gtacttcaac acccaccgga tggtccagga gtacacggag acgtcgtacc ttcccgccca
     1501 tcgcgcgggt tcgcggcttt ccgcgaacga tcattccgcc gcccgggaat tgaccgcgtg
     1561 gcgggagcgc accacgtcgg cgtggccggg gatcacgatc cgtgtggagg aaacgcggaa
     1621 gcacaaggag atgcgcgtgg ggggcacggt gggagtcgcc gtccgcgttc gcctgggggg
     1681 cctcactccc gccgaggtcg ccgtcgagat ccggtacggc ttctacgatg ccgccgggca
     1741 ggtgggcatg gggaccatcc tgtcggcgcg gcacgacggg cgcgatggag acgaggagat
     1801 ctaccgggtg gagatcccgt gcgcgggcag cggccggtac gggttcgccg cgcgggtcct
     1861 tccgcggcac ccggacctcg cgaacgccct cacccccctg cggatgactt gggaggaggc
     1921 ggttcccggc gcttgagggc cggtccgcgg cggtacgctt cggcgcatcg cgacggcgtc
     1981 ctcccgagga ggtagccgat ccggaggcgc cacatgaaca ggacggtccg gagggggccc
     2041 cacgcctccc agcgctggcc cgacgtttcc acgcgcagcg gcagcacgac cgtcttcccc
     2101 gtccggcgca tccgccgcga cagctcgacg tcctccatca gcgggatctc cgggtagccg
     2161 ccgaccgtgc ggaacgcctc cgcccgcacg aagatcgcct ggtccccgct gtacgatcgc
     2221 gatacgaacg cccgcgcccc gatcatccgc cccgtgatcc cgagcgtcgc gcggacgtaa
     2281 cggcctgcgc cgtgagaagc ggccagtcgg acgccgaagg cgccgccgat caccgccggg
     2341 tcttgcagcg cgccgagcac ggcgggcacg gatcccccgg gcagaagggt gtccgcgtgc
     2401 aggaagagga ggacctcccc tccggcgacg gccgcgcccg cgttcatctg cgtcgccctc
     2461 ccccgcggcg ccgagataac ggcgtcggcc cgcccgcgcg cgatctccac cgtgcggtcc
     2521 cggctccccc cgtcgacgac gatcacctcg cacggacccg cctcccggca cgaccggatc
     2581 gcgcgggcga tcgacgcctc ctcgtccagc gcggggatga tcacggaaat cagggcgggc
     2641 gtcatggccg ctcacaccgg caagcgcacc ccactcccgt acggaatgcg ctccctgggg
     2701 taccccgggg tagacatgag gggcacgacg caggggacgt ttgctgggca gaggcgggga
     2761 gcgagtgttt cagttcatcc atacggcgta gcaccgagag cgtcgatgcg ccgcgccggc
     2821 aaggcgcgcg acggaggcgt actgcgaagt acgccgcaag gagcgcaacg cagcaggagt
     2881 ggatgcagcg gcggtcgaat gcaggcggat ggatgaattg aagcactgga ccggatgcga
     2941 ccggggacgc cccctgcgga gaagctctcc tgcagcggga attatggctg cccgcccggc
     3001 ggaaggaact cgcggccctt gcggaggcgc ttcaggaggg tcgggaggaa gaaggagaag
     3061 acgaggagca ggacggggaa gagcaggtcg aacgtcagca ggtccgccgg ccgccggtac
     3121 gtggcgagga catccctcat cgcccctacg aagtaggtga agatgaagac gggcgggagc
     3181 agcccaagca ccgtcccgag gaggtagtcg cggaaccgga tccgcgtcgc cccggcggcg
     3241 tagttgagga cgatgaaggg gaaccagaag atccgcaggt agaagatcac cgagaacccc
     3301 tcgtcggccg ccttccggtc gagccacggc accctggtct cgaggaaccc gcgggcgaca
     3361 ccgtgaagga agtacttccc gaggtagaac gagaccgagg ccccga
//
LOCUS       DASVJL010000134         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1803123, whole genome shotgun sequence.
ACCESSION   DASVJL010000134 DASVJL010000000
VERSION     DASVJL010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803123"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VF357_02635"
     CDS             <1..1073
                     /locus_tag="VF357_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523072.1"
                     /translation="PDAFFPLLAKLPMKKTVVVAISKSGGTAETNAQLALAIAALKKA
                     VGRRWRERLILITDPSKGIFRRMADAEGLKSYAVPPNVGGRFSVLSPVGLFPLAAAGV
                     RVERLLAGAAQMEAIFRHTKGTDNPVRFAAAVYAYYLLVKPKAVQVWFTYGAGLSLLA
                     EWWQQLWGESLGKEREGRSPIGQTPARAVGVTDQHSPLQLYQDGPADKMFTFIRWMTG
                     REKGNVPKAGFAPDMAMLGGRPLRDLFDAEFEGTIGALWSAGRPIVRMEIGKRDEEHV
                     GAFLHFWEWVTAIAGTCAGVDPFDQPGVEEGKRISRALMGEKGTEALRADFAEKVSRI
                     RRAEIRIGEDRPGAPTRRKGRK"
     gene            1074..2906
                     /gene="mutL"
                     /locus_tag="VF357_02640"
     CDS             1074..2906
                     /gene="mutL"
                     /locus_tag="VF357_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX7523073.1"
                     /translation="MTSRIRSLPDDVVNRIAAGEVVERPASVLKELLENAVDSGAGSV
                     EADVSGPFPFSLRVTDDGCGMSREEAELAVRRHTTSKIVSADDLERIGSFGFRGEALP
                     SIASVSRVSVVTRPAEERSGTELVTEGGTILSVRDAGAPCGTTVTVSGLFENVPARRK
                     FLKSERTEMSHLWEVFHGVAIPGEGISFRFIDPRGEFAYESRESALDRAKRHAGDDGQ
                     YLVPVDVSSAFFRIFGWAGLPQVSRAGAGGLWFFINGRRFRDRGLYAAVREAYRGILP
                     GDRLPVLYLFVTCSPREVDVNVHPAKTEVRFRYPRDLNELARHAIGGALGEAPTRAAP
                     PFRVWEERTTPGGLRPAGSPPDMSLEGVTGSPFPLPAAGAERARDLPFAERGDEAADG
                     HGWPSAGAGKDAAGFFAPAGDRFFSSLVPIGQVLMTYLVCEEAGGIVLVDQHAADERI
                     VFSRLKDRYLGKRAPTQRWLDSVEVALPGVLSEADERPAVETFLSRTGFFFEPAGGER
                     IRLTGGPAALPGFDARRWWEELCESLRAQETLPKDLFDADRELWRMACHTAVRGGERV
                     TTERARLLLAELDAAMAAHSCPHGRPVWIRISRARLEALFHRTG"
BASE COUNT          465 a         1061 c         1150 g          514 t
ORIGIN      
        1 accccgacgc tttcttcccc ctcctggcga agctcccgat gaagaagacc gttgtggtgg
       61 ccatcagcaa atcggggggg acggcggaga cgaacgccca gctcgcgctc gcgatcgccg
      121 cgttgaagaa agccgtcggg aggcgttgga gggagcggct gattctgatc accgatcctt
      181 cgaaggggat cttccgtcgg atggcggatg cggaggggct gaaaagctac gccgtgccgc
      241 cgaacgtcgg cggccgcttc tccgtcctct cgccggtggg gctgttcccg ctcgccgcgg
      301 cgggggtccg cgtggagcgg cttctcgcgg gggccgcgca gatggaggcg atcttccggc
      361 acacgaaggg gaccgacaac ccggtccggt tcgccgcggc cgtctacgcg tactaccttc
      421 tggtgaagcc gaaggcggtg caggtctggt tcacctacgg ggcggggctt tcgctgctcg
      481 cggagtggtg gcagcagctc tggggagaaa gcctggggaa ggagcgcgag ggccgctctc
      541 ccatcgggca gacccccgcg cgcgcggtcg gcgtcaccga ccagcactcc ccgctccagc
      601 tttaccagga cggccccgcg gacaagatgt tcacctttat ccggtggatg acgggaaggg
      661 aaaaggggaa cgttccgaag gcggggttcg ctccggatat ggcaatgctc ggcggccgac
      721 cgctgcggga tctcttcgac gcggagttcg aggggacgat cggcgcgctt tggagcgcgg
      781 gacgtcccat cgtgcgaatg gagatcggaa agcgcgacga agagcacgtc ggcgcgttcc
      841 tccacttctg ggaatgggtg acggcgatcg cgggtacgtg tgccggggtc gacccgttcg
      901 accagcccgg cgtggaggag gggaagcgga tctcccgcgc gctgatgggg gagaaaggca
      961 cggaggcgct ccgcgcggac ttcgcggaaa aggtctcgag gatccgccgc gccgagatcc
     1021 gcatcgggga ggatcgtccc ggcgccccca cccgccgcaa gggcaggaag tagttgacct
     1081 cccgcatccg gtccctgccg gacgacgtcg tcaaccggat cgccgcaggg gaggtcgtcg
     1141 aacgcccggc gtcggtcctc aaggagttgc tcgaaaacgc cgtcgactcc ggcgcgggga
     1201 gcgtggaggc ggacgtttcg gggcccttcc cgttctccct ccgcgtcacc gacgacgggt
     1261 gcggcatgtc ccgcgaggag gcggagctcg cggtgcgccg gcacacgacg agcaagatcg
     1321 tctcggcgga cgacctggag cggatcggct ccttcggatt ccgcggcgag gcgcttccgt
     1381 cgatcgcatc ggtctcgagg gtctccgtcg tcaccaggcc cgcggaagag cgaagcggga
     1441 cggaacttgt gacggagggg ggaacgatcc tctccgtccg ggacgcgggc gctccctgtg
     1501 ggacgaccgt caccgtctcg gggctcttcg agaacgtccc cgcccgccgg aagttcctga
     1561 aaagcgagcg caccgagatg tcgcacctgt gggaggtgtt tcacggcgta gcgatccccg
     1621 gcgaggggat ctccttccgg ttcatcgatc cccgcgggga gttcgcgtac gagagccgcg
     1681 agtccgccct ggaccgcgcg aagcggcacg cgggcgacga cgggcaatac cttgtccccg
     1741 tcgacgtctc ctccgccttc ttccggatct tcggctgggc gggactcccc caggtttccc
     1801 gcgcgggggc ggggggtctc tggttcttca tcaacggccg gcggtttcgc gaccgcggcc
     1861 tctacgcggc ggttcgggag gcgtaccggg ggatcctgcc cggcgaccgc ctccccgtcc
     1921 tctacctgtt cgtcacctgc agcccgcggg aggtcgacgt caacgtccac ccggcgaaga
     1981 cggaggtccg cttccggtat ccccgcgact tgaacgagct ggcgcgccac gccatcggcg
     2041 gggcgctcgg ggaggcgccg acgcgcgcgg cacctccttt ccgcgtctgg gaggagcgga
     2101 ccacgccggg agggctaagg ccggcgggat cgccgccgga catgtcgctc gagggcgtca
     2161 ccggttcccc cttccccctt cctgcggccg gcgcggaacg cgcgcgggat ctgccgttcg
     2221 ccgaacgggg cgacgaggcg gccgacggcc atggatggcc tagtgccggg gcaggcaagg
     2281 atgccgcggg ttttttcgcc ccggccggtg accggttctt ttcctccctt gtcccgatcg
     2341 ggcaggtgct gatgacgtat ctggtctgcg aagaggccgg cgggatcgtc ctcgtcgacc
     2401 agcacgcggc cgacgagcgg atcgtctttt cgcgcttgaa ggatcggtac ctcgggaaga
     2461 gggcccctac gcagcgatgg ctcgactccg tggaggtagc cctcccgggg gtcctgtcgg
     2521 aggcggacga gcgcccggcc gtggagacgt ttctctcccg gaccggattt ttcttcgagc
     2581 cggcgggggg ggagcggatc cgcctgaccg gcggaccggc cgcgctgccg ggattcgacg
     2641 cgcggcgatg gtgggaggaa ttgtgcgaat cgctgcgcgc acaggagacc ctccccaagg
     2701 acctgttcga cgcggatcgc gagctgtggc ggatggcgtg ccacacggcc gtgcgcggcg
     2761 gcgagcgggt gacgacggag cgcgcccgcc tcctgctcgc ggagctcgac gcggcgatgg
     2821 ccgcccacag ctgtccccac ggccgcccgg tgtggatccg catctcccgg gcccgtctcg
     2881 aagccctctt tcaccgcacg ggataaatta gtgcaactga tcataattgc ggcaacaaac
     2941 gaccggtggg tactcggact ccgggcgcag cggggggctt cctctccgct gcgctccaga
     3001 cgccgcttcg aggacccccc ccgctgcgcc ctgcgtcccg cacttgatcc gttgcggtaa
     3061 taatgaattg aagcacccag ttccgcttat cgtcctctcc ggtccgacgg cctccgggaa
     3121 atcggccctg gccctcgccc tggggcgcga attccccgtg gagatcgtca acgccgattc
     3181 ccttcaggtg
//
LOCUS       DASVJL010000135         5612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_676604, whole genome shotgun sequence.
ACCESSION   DASVJL010000135 DASVJL010000000
VERSION     DASVJL010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676604"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /gene="radA"
                     /locus_tag="VF357_02645"
     CDS             <1..1404
                     /gene="radA"
                     /locus_tag="VF357_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX7523074.1"
                     /translation="RRGGARGGEEVAKVKVEFACTACGTSSPKWVGKCPGCGEWNTLV
                     EEVASSSSPRQRTAGMPDFPTSRPMRLTEVAETASSRTSCGIAEFDRVMGGGVVPASA
                     TLLGGDPGIGKSTLLLQAARGLARHGKPVLYVSGEESEAQVKLRASRLGVTDAGIYLM
                     SETSLERILSATGELSPGTLIVDSIQTVFSSDLPGAPGSVGQVRECAARLAFFGKRAG
                     VSVFLVGHVTKEGAIAGPRVLEHIVDTVLYFEGEKGHPYRILKAVKNRFGSTNEIGVF
                     EMRAEGLTEVADPSGMFLSERSDETSGTLVFPAIEGTRPLLVEVQALVSQSFLAMPRR
                     TTLGVDYNRVVLMCAILEKKAGVSLYNQDVFVNVAGGIRVEEPAADLALCCAVAGSFK
                     DRVIPPGTAVFGEVGLGGEVRAVPMAEMRLNEAAKMGFSRVVLPASNAERLGAKFPLE
                     LLPVRHVKEALSRVAGK"
     gene            1411..1860
                     /locus_tag="VF357_02650"
     CDS             1411..1860
                     /locus_tag="VF357_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX7523075.1"
                     /translation="MGKRYWEDLVEGERFTCRSVEFDREGIVAFGKRYDPLPFHADED
                     AARRSLFGDVIASALHTVSACTRAVVEAHGEIAILSGLSIDEIRTFHPVRPGDLLTVE
                     GRWDGLRRSRSKPDRGIAEVKCRVVNQKGETVMEYGYRYLVACRPKP"
     gene            1905..2468
                     /locus_tag="VF357_02655"
     CDS             1905..2468
                     /locus_tag="VF357_02655"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX7523076.1"
                     /translation="MIRCGWADRGELERAYHDREWGVAVHDDRRLFEFLVLEGAQAGL
                     SWSTVLKKREGYRKAFRNFDVGVVSGFSESDVSMLFSDPGIIRNRLKIRAAIGNAKAF
                     LRVQEEFGCFDNYIWRFVNGNPVRNAWNRMTEIPCSTPESEAMSKDLRNRGFKFVGST
                     ICYAFMQAVGMVNDHVVECFRYRNLSR"
     gene            complement(2443..3054)
                     /locus_tag="VF357_02660"
     CDS             complement(2443..3054)
                     /locus_tag="VF357_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX7523077.1"
                     /translation="MYDRKDAYYRHAKKEGYRSRAAYKLTQIAAKEKAVRSGDRVIDA
                     GAAPGGWSQILLGMVGAKGKIAAVDILPMATLPGENFRFWQRDLTDPALPAELLEFLG
                     GKADAVVSDAAPNTTGSSFTDQARSADLVRAVFGLTRETLREGGTFLAKIFGGAEADA
                     VFREMKPSFADLRRLRPEATRKESFELYFLGKGYKATSRDSCT"
     gene            3117..4112
                     /locus_tag="VF357_02665"
     CDS             3117..4112
                     /locus_tag="VF357_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7523078.1"
                     /translation="MSRRRTTIDIDGVFRYLSRDLRTVERALVSSLASPVPLIHVVGK
                     HITLSGGKRVRPAILLLAADACGYRGPRRTVMSVVTEYMHTATLLHDDVVDTGTVRRG
                     KPSANVVFGNSVSVLVGDFLFARASQLMTDDGDIDVLGIYARTLVHLTEGEVLQLMKA
                     RDGGITEKEYLTVVFCKTASLIAAAAETGAVLAGADPATRKAMFGFGKSIGIAFQLVD
                     DILDFTGTEKSLGKRPLQDLREGKVTLPLLHALRAAGAADRERARCALAKKEPGDREV
                     AFLVGLVARHDGIGYAASRAREYVRRGKRYLAVLPDSPARSALVALSDYVTSRTS"
     regulatory      4209..4369
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4496..5458
                     /locus_tag="VF357_02670"
     CDS             4496..5458
                     /locus_tag="VF357_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX7523079.1"
                     /translation="MGRVRIAVARRFLAGFLPAVALLSSAVDRPAFAADRFPLALKDD
                     RGVTVRLAAPPRRVVSLAPSLTEIVFLLGCEGSLVGVTRFCNVPAAASGLPKIGGVSD
                     PDVERIVALSPDLVLCTTDGNPRDKVRAVEEMGIPCFAVAPQDLDAVFAAIERLGVLL
                     GAADRGRAEAGALRRRARLASSSSSDAEKPAVLFVVSTTPIIAAGEGTFMDELVRLAG
                     GRNAAARFSGRYPRLSVEGLVAARPDVIFVAGMSGVERFPPEVTRWKEVPAFRDGAVI
                     TLDGDLVTRPGPRLVSALERVSAALANWRASRPKAPAADAGGKR"
BASE COUNT          851 a         1829 c         1997 g          935 t
ORIGIN      
        1 cgacgaggtg gcgctcgagg tggcgaagaa gtggcgaagg taaaggtcga gttcgcctgc
       61 acggcgtgcg ggacctcttc tcccaagtgg gtggggaagt gcccggggtg cggggagtgg
      121 aacacgctgg tcgaggaggt cgcctcctcc tcctcgccgc ggcagcgcac cgccggcatg
      181 cccgacttcc ccacctcgcg cccgatgcgc ctcaccgagg tcgcggagac cgcctcgtcg
      241 cggacgtcgt gcgggatcgc cgagttcgac cgggtgatgg ggggcggcgt ggtccccgcc
      301 tcggcgacgc tcctgggcgg cgacccgggg atcgggaagt ccaccctcct cctccaggcg
      361 gcgcggggcc tggcccggca cgggaagccg gtcctgtacg tctcgggcga ggagtcggag
      421 gcgcaggtga aattgcgcgc ctcgcggctg ggcgtgaccg acgccggcat ctacctgatg
      481 tcggaaacgt ccctcgagcg gatcctctcc gccaccggag agctctctcc agggacgctg
      541 atcgtcgact cgatccagac cgtgttctct tcggatcttc ccggggcgcc gggttccgtc
      601 ggccaggtgc gggagtgcgc cgcgcgtctc gccttcttcg ggaagagggc gggggtgtcg
      661 gtcttcctcg tgggacacgt gacgaaggag ggggcgatcg cggggccgcg ggttctcgag
      721 cacatcgtgg acacggtcct ctacttcgag ggggagaagg gacacccgta ccggatcctg
      781 aaggcggtga agaaccggtt cggctcgacg aacgagatcg gcgtcttcga gatgcgggcc
      841 gaggggctga ccgaggtcgc cgacccgtcg gggatgttcc tctccgaacg gtcggatgaa
      901 acgtcgggaa cgctcgtctt cccggcgatc gaggggaccc ggccgctgct ggtcgaggtg
      961 caggcgctcg tttcgcagtc gttcctcgcg atgccgcgcc gcacgacgct gggcgtcgac
     1021 tacaaccgcg tggtcctgat gtgcgccatc ctcgagaaga aggcgggcgt ctccctctac
     1081 aaccaggacg tcttcgtcaa tgtggcggga gggatccggg tcgaggaacc cgcggccgac
     1141 ctcgccctgt gctgcgccgt ggccggaagc ttcaaggacc gggtgatccc gccggggacg
     1201 gcggtgttcg gcgaggtggg actggggggc gaggtgcgcg cggtgccgat ggccgagatg
     1261 cgcctgaacg aagccgccaa gatggggttc tcccgcgtcg ttctgccggc gtccaacgcg
     1321 gagcgcctgg gcgcgaagtt tcccctcgaa ctgctcccgg tgcgccacgt gaaggaagcc
     1381 ctctcccgcg tcgcggggaa gtaagccggc atgggcaaga ggtactggga ggatctggtc
     1441 gagggggagc ggttcacgtg ccggtccgtg gagttcgatc gggaagggat cgtcgcgttc
     1501 gggaagaggt acgacccgct cccgttccac gcagacgaag acgccgcgcg ccggtcgctc
     1561 ttcggcgacg tgatcgcatc cgccctgcac accgtgtccg cctgcacacg agcggtcgtc
     1621 gaagcccacg gagagatcgc catcctctcg ggcctgagca tcgacgagat ccgcacgttc
     1681 cacccggttc gcccgggcga cctcctgacg gtcgaggggc gttgggacgg cctccggcgg
     1741 tcgcgcagca agccggaccg ggggatcgcc gaagtgaagt gccgggtcgt gaaccagaaa
     1801 ggcgagaccg tgatggaata cggatatcgc tacctggttg cctgccgacc gaagccctga
     1861 tggacggcgg acgatcacgg cgatcgacgg gccgggggtg acggatgata cgttgcggtt
     1921 gggccgacag gggcgagctc gaacgggcct atcacgacag ggaatggggg gtagcggttc
     1981 acgacgacag acgcctattc gagtttctgg tcctggaagg ggcgcaggcc ggattgagct
     2041 ggtctaccgt cctcaagaaa cgggaggggt atagaaaagc gtttcggaat ttcgatgtcg
     2101 gtgtcgtttc cggattctcg gagagcgatg tctcgatgtt gttttccgac cccgggatca
     2161 tcagaaacag gctcaagatc cgcgctgcca tcggcaacgc caaggcgttc ctgcgggtac
     2221 aggaagaatt cggatgtttc gacaattaca tctggcggtt cgtcaacggg aaccctgtcc
     2281 ggaatgcatg gaacaggatg acggaaatac cgtgcagcac ccccgagtcc gaggccatga
     2341 gcaaggattt acgcaacagg gggttcaagt tcgttggctc gacgatctgt tacgcattca
     2401 tgcaggcggt tggcatggta aacgatcatg tcgtggaatg tttcaggtac aggaatctct
     2461 cgaggtagct ttgtaccctt tccccaggaa atagagctcg aacgactcct tgcgcgtcgc
     2521 ttcgggacgg aggcggcgga ggtccgcgaa ggacggcttc atttcccgga agactgcgtc
     2581 ggcttccgcg ccgccgaaga tcttggcgag gaaggtcccg ccttcgcgca gtgtctcccg
     2641 cgtaagcccg aagacggccc ggacgaggtc cgcggaccgc gcctggtcgg tgaaggagga
     2701 tccggtcgtg ttgggggcgg cgtccgagac gaccgcgtcg gccttccccc cgaggaattc
     2761 gagaagctcg gccggcaagg cggggtcggt caggtcccgt tgccagaagc ggaagttttc
     2821 cccgggcagc gttgccatcg gaaggatgtc gacggcggcg attttcccct tggcgcccac
     2881 catgccgagg agaatctggc tccagccgcc cggcgcagcc cccgcgtcga tcacccggtc
     2941 cccggagcga accgccttct ccttcgcggc gatctgggta agcttatacg ctgcccggga
     3001 gcgatacccc tcctttttcg catgccggta atatgcgtct ttccggtcgt acatcttctc
     3061 atcgtatcga aaccgccggt agttgacact ccgaaatcct gtgcgatagt gtcaacgtgt
     3121 cccgaaggag aacgaccatc gacatcgacg gcgtcttccg ctacctgagc cgcgacctgc
     3181 gcacggtcga gcgggcgctc gtctccagcc tggcctcccc tgtcccgctc atccacgtcg
     3241 tcggaaagca catcaccctg tccggcggga agcgggtccg ccccgcgatc ctcctcctgg
     3301 ccgcggacgc ctgcggttac cggggcccgc ggcggaccgt gatgtccgtc gtgacggagt
     3361 acatgcacac ggcgacgctc ctgcacgacg acgtggtcga caccgggacg gtgcgccgcg
     3421 gcaaaccgtc ggcaaacgtc gtgttcggca actccgtgag cgtcctcgtt ggcgatttcc
     3481 tcttcgcccg cgcctcccag ttgatgacgg acgacggcga catcgatgtt ctcgggatct
     3541 acgcgcgcac cctggtccac ctcacggaag gggaggtgct tcagttgatg aaggcgcgcg
     3601 acggggggat cacggagaag gagtacctga ccgtcgtctt ctgcaagacc gcgtcgctga
     3661 tcgccgcggc cgccgaaacc ggggcggtgc tcgccggggc cgaccccgcg acgcgcaagg
     3721 cgatgttcgg gttcgggaaa tcgatcggga tcgccttcca gttggtcgac gatatcctcg
     3781 acttcacggg gaccgagaag tcgctcggga agcgccctct gcaggatttg cgggagggga
     3841 aggtgaccct tccgctcctg cacgcgctgc gggcggcggg ggccgcggac cgcgagcggg
     3901 cgcggtgcgc cctcgcgaag aaggagcccg gggaccgcga ggtcgccttc ctcgtcggcc
     3961 tggtggcccg ccacgacggg atcgggtacg cggcctcccg ggcgagggag tacgtccggc
     4021 gcgggaagcg gtacctcgcc gtcctccccg actcgccggc ccggtccgcc ctggtcgccc
     4081 tgtccgacta cgtcacctcc cggaccagct aaccgttcca gtgtagcggt gcgatggaga
     4141 ttccgccttg acgcatgggg cgccgggcgg cagaatggtc acgcagatcg gaacgttccc
     4201 gtcggggcaa gggaagatgg tgcgagtcca tcgctgcccc gcaactgtaa ccggtgacga
     4261 aatccgcaac ggagccactg cccgcgtgac gagagcgcgg gcgggaaggc gcggagagta
     4321 ggggggcggc ccttcggggg ccgccgggta gccggaagcc aggagacctt cccggcgggg
     4381 agagatcccg agacgccttt cgcgggagaa gggccggggc gctttcgccg tcaggacgcg
     4441 cgtttcatcc cccatccgtt tttccgcaag gcatcgggcc gggggaggat ctattttggg
     4501 ccgcgtccgg atcgccgtcg cacgccgttt tctcgcgggg ttcctccccg ccgtcgccct
     4561 cctttcgtcc gcggtcgatc gtcccgcgtt cgcggcggac cggttccccc tcgcgttgaa
     4621 ggacgaccgg ggcgtcaccg tccggctcgc cgcccctccc cgccgcgtcg tctcccttgc
     4681 ccccagcctc accgagatcg tcttcctcct cggttgcgaa ggttccctcg tcggcgtcac
     4741 ccggttttgc aacgttccgg ccgcggcctc cggcctgccg aagatcgggg gcgtgagcga
     4801 tcccgacgta gagcggatcg tcgcgctgtc gccggatctc gtcctgtgca cgacggacgg
     4861 gaatccccgg gacaaggtgc gggccgtcga ggagatgggg atcccgtgct tcgccgtggc
     4921 cccgcaggat ctggatgcgg tcttcgcggc gatcgagcgc ctgggcgtcc tcctcggcgc
     4981 cgcggaccgg ggacgcgccg aggccggggc gcttcgccgc cgcgcgcggc tcgcgagctc
     5041 ctcctccagc gacgcggaaa agcccgcggt gctcttcgtc gtctccacaa cgccgatcat
     5101 cgccgccggc gaaggcacct tcatggacga actggtgcgt ctcgccggcg ggaggaacgc
     5161 cgccgcgcgc ttttccggaa ggtacccgcg gctctccgtg gaagggctgg tcgccgcccg
     5221 gccggacgtg atcttcgtcg cggggatgtc cggcgtcgag cggttcccgc cggaggtcac
     5281 ccgctggaaa gaggtccccg cgttccggga cggcgccgtg atcaccctcg acggggacct
     5341 tgtgacccgc cccggcccgc ggctggtgag cgcgctcgag cgggtatccg cggcccttgc
     5401 aaactggcgg gcgagccgcc cgaaggcgcc ggcagcggac gcggggggga agcggtgacg
     5461 ggcggcggga aaaggagcgg cgcggttttt cccgtgctcg ccgccgcgct ccttggcgcg
     5521 ctggccctct ccgcggccac cgggccggtg tcgatctccc cgcgcgcggc cttcgacgcg
     5581 atcttccggg gaacggacga cactcccgcc cg
//
LOCUS       DASVJL010000136         4836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_208366, whole genome shotgun sequence.
ACCESSION   DASVJL010000136 DASVJL010000000
VERSION     DASVJL010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208366"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /gene="secD"
                     /locus_tag="VF357_02675"
     CDS             <1..1288
                     /gene="secD"
                     /locus_tag="VF357_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX7523080.1"
                     /translation="GDFASRAQATLKEEFPSLDVSLGEVKADGATVIARMKPAEIQAI
                     RTNAVVQGVETIRNRIDQFGVREPQIIAEGEDRIVVQLPGVKDQQRAIELVGKTALLE
                     FKLVDEGASLEEALKGNVPEDDQVLYQKSVDPQSGRATKTSILVKKRALLTGDTIKTA
                     KVNFGNQGGGAHVSLSFDTRGAKVFDRVTAENVKRRLAIVLDDTVYSAPVIQERISGG
                     EAQITGNFTPEEASDLAIVLRAGSLPAPVKVIQNVSIGPSLGLDSIQKGVRAALIGAV
                     LVVLFMAFYYRFAGMVADFALVFNILFLLSGMAAFSATLTLPGIAGIILAIGMAVDSN
                     VLIFERIREEIRAKKSVRAAIDAGYDKAFWTVVDSHVTTLITAVILFQFGTGPIKGFA
                     VTLSMGVAINLFTALVCTKVVFDYLNAKKPMQALSI"
     gene            1313..2230
                     /gene="secF"
                     /locus_tag="VF357_02680"
     CDS             1313..2230
                     /gene="secF"
                     /locus_tag="VF357_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX7523081.1"
                     /translation="MIELVKNTKIDFMGLKKYAFSFSAIIVILGIVGTVQIYRGKANL
                     GIDLAGGTSIQLKFQKPFSMNEIRSLLANAGHGEATLQEVSGENILLVKLRALGKQDE
                     KMVAEPMVNLLRQSLPGNPFVVESVSEIGPAIGHKLRRDAELAMLISALAIIVYLAWR
                     FEFKFGVAAAIATFHDIFSIVGIFWVLGIEMDLLFITALLTIGGYSLTDTVVVFDRIR
                     ENIRLRKKGTFSETINLSVNEVLSRTIVTSMTVFIAVIALLVFGGLVLRNFALALAMG
                     IVVGTYSSVFVASPIVAVWRGEKMIEVKR"
     gene            2244..4007
                     /gene="recJ"
                     /locus_tag="VF357_02685"
     CDS             2244..4007
                     /gene="recJ"
                     /locus_tag="VF357_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX7523082.1"
                     /translation="MKGAAPREWILRSPDPAAVRELSARHGLAPAASKVLVNRGIVEP
                     RDVERYLGGTLADLPDPSLLKDADKAARRIAAAGARGEPLLIYADYDADGATGAACLY
                     LFLKRIFPDLPVRIQQNDRRKDGYGLQTHVLAPAAREGYRLVVTVDCGISDVAAVREA
                     AREGVEVIVTDHHLQGETVPEAFAIVDPMQPECAFPGKELAGVGVVFLLVCALRKAVR
                     EIAGLGLLPEPPLRPYLDLVALGTVADMAALRGGNRLLVREGLREIRKSPRPGIEALF
                     EASGVPYAAATEMDLGFRVGPRLNAAGRVGDATRSSRILVSESRDDAVRLARELNGDN
                     ALRQREEERIVVAVEAALAAAGEIPPAIVLSDPAWNAGVLGIVASKVLERYGRPVVLL
                     QEEDGWAKGSCRSVEGFHIVSALSRLSHLLTRYGGHAQAAGLALPLEHLRAFREGLAE
                     IADRHARETPFVRRRSIDAQLRVAEITPDLLSDLDRLRPFGVGNEEPLFLLPNIRVAA
                     ISRIGAGGRHLRFAVEEDGRRLSGVAFRRAEIPVDAEGRSDLLFAVQENVYRGARSLQ
                     LLLRDARPAGDPVLCGGTPPG"
     gene            4048..>4836
                     /gene="speB"
                     /locus_tag="VF357_02690"
     CDS             4048..>4836
                     /gene="speB"
                     /locus_tag="VF357_02690"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX7523083.1"
                     /translation="MVPYSVTFGGISGRYASWKAATFVVIPFPVDMTTTYMTGTRNGP
                     RAILDASSHMELFDEENKIEPYRAGIFVSTEVPVSLAGPVPALKEVERRVNATLRAGK
                     FPVLLGGEHSGTAGAVVALRKRYEDLTVLQFDAHTDLRDTYLGTPHNHACVGRRIVDA
                     GARLVQVGIRSISEEEDRFLKKSEEVKTFYASEVRDNVADVTKGIVSSLSENVYISID
                     LDVFDPGIMPAVVTPEPGGLSWFEGIDILRDVMRSNRNIVGFDVM"
BASE COUNT          781 a         1562 c         1659 g          834 t
ORIGIN      
        1 gggcgacttc gcctcccgtg cgcaggccac actcaaggag gagtttcctt ccctcgacgt
       61 gagcctgggc gaagtgaagg ccgacggggc gacggtgatc gcgcggatga agcccgcgga
      121 gatccaggcg atccggacga acgcggtggt gcagggggtg gagacgatcc ggaaccggat
      181 cgaccagttc ggtgtccggg aaccccagat catcgcggag ggggaggacc ggatcgtcgt
      241 gcagcttccg ggtgtcaagg accagcagcg cgcgatcgag ctcgtcggga agacggccct
      301 gctcgagttc aagctggtcg acgagggtgc gagcctggaa gaggccctca aggggaacgt
      361 ccccgaggac gaccaggtgc tctaccagaa gtcggtcgac ccgcagagcg gccgggcgac
      421 gaagacctcg atcctcgtga agaagcgggc gctgctcacc ggcgatacga tcaagacggc
      481 gaaggtgaac ttcggcaacc agggcggggg cgcgcacgtc tccctctcct tcgacacacg
      541 gggcgcgaag gtcttcgacc gggtgaccgc cgagaacgtg aagcgccgcc tggcgatcgt
      601 cctcgacgac acggtctact ccgcgcccgt catccaggag cggatctctg gaggcgaggc
      661 gcagatcacc gggaacttca ccccggagga ggcgtccgat ctcgcgatcg tcctgcgggc
      721 cggctcgttg ccggcgccgg tcaaggtgat ccagaacgtc tccatcggac cgtctcttgg
      781 cctcgattcg atacaaaagg gtgtgcgggc ggcgctgatc ggcgccgttc tcgtcgtctt
      841 gttcatggcg ttctactacc ggttcgccgg gatggtggcg gacttcgccc tcgtcttcaa
      901 catcctcttc ctcctgtcgg ggatggccgc cttctcggcg accctcaccc tgcccgggat
      961 cgcggggatc atcctggcga tcgggatggc ggtcgactcg aacgtcctga tcttcgagcg
     1021 gatccgcgag gagatccgtg cgaaaaagtc cgttcgggcg gcgatcgacg caggatacga
     1081 caaggcattc tggaccgtgg tggattccca cgtgaccacg ctgatcacgg cggtgatcct
     1141 cttccagttc ggcaccgggc cgatcaaggg gttcgccgtg acgctctcca tgggcgtggc
     1201 gatcaacctc ttcaccgcgc tcgtgtgcac caaggtcgtg ttcgactatc tcaacgccaa
     1261 aaaaccgatg caggcgctga gcatttaacc gaaacgggga cggaatacga cgatgatcga
     1321 actggtaaaa aacaccaaga tcgacttcat gggtctgaag aaatacgcct tttctttctc
     1381 cgcgattatt gtgatcctcg ggatcgtggg caccgtgcag atctaccgcg ggaaggcgaa
     1441 cctcgggatc gacctggcgg gcgggacgtc gatccagctg aaattccaga agccgttctc
     1501 catgaacgag atccggtctc ttctcgcgaa cgcggggcac ggtgaggcga ccttgcagga
     1561 agtttcgggg gagaacatcc tcctggtcaa gctgcgcgcc ctgggcaagc aggacgaaaa
     1621 gatggtcgcc gaaccgatgg tgaatctcct gcggcaatcc ctcccgggca accccttcgt
     1681 cgtcgagagc gtttcggaga tcgggcctgc catcgggcac aagctgcgcc gggacgccga
     1741 gctggcgatg ctgatttccg cgctggcgat catcgtctac cttgcgtggc ggttcgagtt
     1801 caagttcggc gtcgccgcgg cgatcgccac gttccacgac atcttctcga tcgtcgggat
     1861 cttctgggtt ctcgggatcg agatggacct gctcttcatc accgcgctgc tcacgatcgg
     1921 ggggtactcg ctgaccgaca cggtcgtcgt cttcgaccgg atccgggaga atatccggct
     1981 ccggaagaag gggaccttct ccgagacgat caacctgagc gtcaacgagg tcctcagccg
     2041 gaccatcgtc acctcgatga ccgtcttcat cgccgtcatt gcgctgctgg tcttcggcgg
     2101 gcttgttctg cgcaacttcg cgctggctct cgcgatgggg atcgtggtcg ggacgtactc
     2161 ctcggtcttc gtcgccagcc cgatcgtcgc cgtctggcgc ggcgagaaga tgatcgaagt
     2221 gaagaggtga tcggagcggg ggcttgaaag gggcggctcc gagggagtgg atcctccgct
     2281 cccccgatcc ggccgccgtc cgggagctgt cggcccgcca tggcctggcc cccgccgcat
     2341 cgaaggtgct cgtcaaccgc gggatcgtgg aaccgcgtga cgtcgaacgc tacctcggcg
     2401 ggaccctcgc cgatctcccc gacccttccc tcctcaagga tgccgacaag gccgcgcgcc
     2461 gcatcgcggc cgccggcgcg cgtggggaac cgttgctcat ctacgccgac tacgacgccg
     2521 acggcgccac cggcgccgcc tgcctctacc ttttcctgaa gcggatcttc cccgatctcc
     2581 ccgtacggat ccagcagaac gaccgccgca aggacggcta cggcctgcag acgcacgtgc
     2641 ttgcgcccgc cgcccgggaa gggtatcgcc tcgtcgtcac cgtcgactgc gggatctccg
     2701 atgtcgcggc cgtgcgggag gcggcccggg agggggtcga ggtgatcgtg acggaccatc
     2761 acctccaggg cgaaaccgtt ccggaggcgt tcgccatcgt cgacccgatg cagccggaat
     2821 gcgcgttccc ggggaaggag ctggcgggcg tgggggtcgt cttcctgctc gtgtgcgccc
     2881 tgcgcaaggc ggtgcgggag atcgcgggat tagggcttct gccggagccg ccgctgcggc
     2941 cgtacctcga cctggtcgcc ctgggcacgg tcgccgacat ggcggccctc cggggaggaa
     3001 accggctcct cgtccgggag gggctccggg agatccgcaa gtcgccccga ccggggatcg
     3061 aagcgctgtt cgaggcgtcg ggcgtgccgt acgccgcggc gacggagatg gatctcggat
     3121 tccgggtcgg gccgcgcctg aacgccgcag gccgggtggg agacgcgacg cgcagctccc
     3181 ggatcctcgt gtcggaatcc cgggacgatg ccgtgcgcct tgcccgggaa ctgaacgggg
     3241 acaacgcgct gcggcagcgg gaggaggagc ggatcgtcgt cgccgtggaa gccgccctcg
     3301 cggcggcggg ggagatcccc ccggcgatcg tcctgtcgga ccccgcctgg aacgctgggg
     3361 tgctcgggat cgtcgcctcg aaggtcctcg agcggtacgg ccgcccggtc gtgctgctcc
     3421 aggaggagga cggctgggcc aaggggtcgt gccgcagtgt cgaggggttc cacatcgtgt
     3481 cggccctgtc ccgcctttcc catctgctca cgcggtacgg tggacacgcg caggcggccg
     3541 gcctcgccct tcctctcgag cacctccgcg ccttccgcga gggccttgcg gagatcgccg
     3601 accggcacgc gcgggagacc cccttcgtac ggcgccggtc gatcgatgcg caactgcgcg
     3661 tcgccgagat cactccggac cttctgtccg atctcgaccg ccttcgaccc ttcggcgtcg
     3721 ggaacgagga gccgctgttc ctcctgccga acattcgcgt cgcagcgatc tcccgcatcg
     3781 gggcgggggg acgccatctc cggttcgcgg tggaagagga cgggcggcgt ctttccggcg
     3841 tggcgttccg ccgggcggag atccccgtag atgccgaggg ccgctcggat ctgctcttcg
     3901 ccgtccagga aaacgtctac cgcggcgcgc gttcgctgca gcttctgctg agggacgcga
     3961 ggccggccgg ggacccggta ttgtgcgggg gaacgccgcc ggggtagaat ctgcctcacc
     4021 ccaaccgttt cacaggaggt cttcaccgtg gtcccatact ccgtaacgtt cggagggatt
     4081 tccggcaggt atgcgtcgtg gaaggccgcg accttcgtcg tgatcccgtt cccggtcgac
     4141 atgaccacga cgtacatgac ggggacgcgg aacggcccgc gcgccattct cgacgcctcc
     4201 tctcacatgg agctgttcga cgaggagaac aagatcgagc cgtaccgggc cgggatcttc
     4261 gtctccacgg aggtcccggt ctccctggcg gggccggtcc cggccctgaa ggaagtcgag
     4321 cggagggtga atgccacgct gcgggcgggc aagttcccgg tgctgctcgg cggggagcat
     4381 tccggaacgg cgggagcggt ggtcgcgctg cggaagcggt acgaagactt gaccgtgttg
     4441 caattcgacg cccacacgga ccttcgagac acgtatctcg gtacgccgca caaccacgcg
     4501 tgcgtcggcc ggcggatcgt cgatgcgggg gcccgtctcg tccaggtcgg catccggagc
     4561 atttcggagg aggaggaccg cttcctgaag aagtcggagg aggtgaagac gttctacgcc
     4621 tccgaagtcc gtgacaacgt ggccgacgtc acgaagggga tcgtcagctc cctctcggag
     4681 aatgtctaca tctcgatcga cctcgacgtg ttcgacccgg ggatcatgcc cgccgtcgtc
     4741 acgcccgagc ccggcgggct ctcctggttc gaggggatcg acatcctgcg ggacgtgatg
     4801 cgttcgaacc ggaacatcgt cgggttcgac gtgatg
//
LOCUS       DASVJL010000137         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1614302, whole genome shotgun sequence.
ACCESSION   DASVJL010000137 DASVJL010000000
VERSION     DASVJL010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614302"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..1162
                     /locus_tag="VF357_02695"
     CDS             392..1162
                     /locus_tag="VF357_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HEX7523084.1"
                     /translation="MSASTQAQALNALVFHFKHVVGTPIGDLGEFQRSKRPRKLPVVL
                     SREEVRRLLDAVEDPYRLPTALLYGAGLRLMEALRLRVKDIDLDRRQISVRDGKGQKD
                     RVTVLPERWRERLAAHLVDVRKNHARDMELGYAGTTFWPALERKYPGAPREWAWQYVF
                     PASRLYVESDTGKTRRHHLHETALQRAVRFAARKADIAKPVGCHTLRHCFATHLLESG
                     ADIRTVQELLGHSDVKTTMIYTHVLNRPGLAVRSPEDG"
     gene            complement(1171..1911)
                     /gene="truA"
                     /locus_tag="VF357_02700"
     CDS             complement(1171..1911)
                     /gene="truA"
                     /locus_tag="VF357_02700"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX7523085.1"
                     /translation="MRRVKLAVAYDGTAYAGWQVQPNGPTIQAAVEEVLARILQEPVR
                     VRAAGRTDAGVHAREQVADFADAGVRDLATIVHGGNALLPPDIRILSASAVPETFDAM
                     RHATEKEYRYFLYLSPVASPFLSRYAWHIEKPLDLTAVRQGLSHLVGEHDFTSFRGQG
                     CTANSPVRKVFRAEISPHDVPGLFSIDVAGAGFLRHMVRNIVGTVVNAGKGKHSADHV
                     GEILRARDRSAAGVNAPPHGLFLWRVSY"
     gene            complement(2161..>2434)
                     /locus_tag="VF357_02705"
     CDS             complement(2161..>2434)
                     /locus_tag="VF357_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEX7523086.1"
                     /translation="KKITREKARELIAKFPGCQVMDDPANNVYPMPLFAAGKDDCYVG
                     RIREDDSAENCLNLWVCGDQLRKGAALNAIQIAEVLAQKYLQLAAV"
BASE COUNT          412 a          811 c          811 g          400 t
ORIGIN      
        1 gagcaccccc ggagtcgtgg gccgcgccgt tccataaggc ccttctcttg cgcggtcgtc
       61 ccgaagcacg cgtaaagtgg tacatcgtct gggcgaggcg cttttccgtt ttccttcccg
      121 gcaggtcgct tcacctggca agtcgcgagg acaccgaggg attccttacc acactcgcct
      181 cctcgccagg atcgccgctt ggcaggtgga gcaggcaacg gatgccctga ccatactcct
      241 tggtagcgta ttcggtcaag agtgggccag gacgatccgg attccggccc ctcctccacc
      301 ccccgacatc ccgcttccca agggagacga cccggctgat catttacgat acgcgatccg
      361 ttgccggcgc tattcggtac gtaccgaaca gatgtcggcg tcgacgcaag cgcaggcgct
      421 taacgcgctc gtattccact tcaagcatgt cgtcggaacg ccgatcggcg acctcggaga
      481 gttccaacgt tcgaagcgcc cccggaagtt gccggtcgtc ttaagccggg aggaggtccg
      541 gcgtctgctg gatgccgtcg aggatccata tcgtctcccg acggcgctgc tttatggcgc
      601 gggcctgcgg ctcatggagg cgctgcggtt gcgggtgaag gacatcgacc tggatcgccg
      661 ccagatctcg gtccgggacg ggaaaggaca gaaggaccgt gtgacggtcc ttcccgagcg
      721 gtggcgggaa cggctcgccg cccatcttgt cgatgtccga aagaaccacg cccgggacat
      781 ggaactcgga tacgccggga cgaccttctg gcccgccctt gaacgcaaat accccggcgc
      841 tccccgcgag tgggcctggc aatacgtctt ccctgcgtcg cgactgtatg tcgagtcgga
      901 cacgggaaaa acacggcgac accacctgca cgagacggcc ctgcagcggg ccgtccggtt
      961 cgccgcgcgc aaggcggata tcgcgaagcc cgtcggctgc cacacgctgc ggcactgctt
     1021 cgccacccac cttctcgaat cgggcgcgga catccgcacc gtgcaggaac tgctcgggca
     1081 cagcgatgtc aagacgacga tgatctacac ccacgtcctg aaccggccgg gcctggcggt
     1141 tcgcagcccc gaggacgggt aggcgggccc tcagtacgat acgcgccaga ggaagaggcc
     1201 gtggggaggg gcgttgaccc cggcggcgga ccggtcgcgc gcccgaagga tttctccgac
     1261 gtgatccgcg gaatgcttcc ccttccccgc gttcaccacc gttccgacga tgttgcgcac
     1321 catgtggcga agaaatcccg cgccggccac gtcgatggag aacagccccg ggacgtcgtg
     1381 cggggagatc tccgcccgaa agaccttgcg gaccggcgaa ttcgccgtgc agccttgccc
     1441 acggaacgag gtgaagtcgt gttccccgac caggtgcgac agcccctgcc gcaccgcggt
     1501 caggtcaagg ggcttctcga tgtgccatgc gtaacgggag aggaacgggg aggcgacggg
     1561 agagaggtac aggaaatacc ggtactcctt ctccgtcgcg tggcgcatcg cgtcgaacgt
     1621 ctccggcacg gcggaagcgg agaggatgcg gatgtcggga ggaagaaggg cgtttccgcc
     1681 gtggacgatc gtcgcgaggt cccgcacgcc ggcgtcggcg aaatcggcga cctgctcccg
     1741 ggcgtgcacg cccgcgtcgg tgcggcccgc ggcccgaacg cgcaccggct cctgcaggat
     1801 ccgggcgagc acctcctcca ccgccgcctg gatggtcggc ccgttcggct gaacctgcca
     1861 ccctgcgtac gccgtcccgt cgtacgcgac ggcgagctta acccgccgca ccgtcggcgc
     1921 cttcctcgtt ccatatccgg gactttccga gatcggtcag gaggcgccgc ggagcgacat
     1981 gcccctcccg agcgggtagc gagggacggg gcatggagcg aagaaagagg cgactcctga
     2041 ccgatcggtg gaagtcccgg catacgggcg aggagggggg gaaaaaaccg ggagcggagg
     2101 aagatccccc gctcccggcg tacgttggtt cgttcggggc cacgcgtccc ccggcggcgg
     2161 tcagacggcc gcgagctgga gatatttctg cgcgagaacc tcggcgatct ggatggcgtt
     2221 gagggccgcc cccttccgga gttggtcgcc gcagacccag aggttgaggc agttttcggc
     2281 gctgtcgtcc tcgcggatcc ggccgacgta gcagtcgtcc ttccccgccg cgaagagcgg
     2341 catcgggtag acgttgttcg ccgggtcgtc catcacctgg cagccgggga acttcgcgat
     2401 caattccctc gccttctccc gcgtgatctt cttt
//
LOCUS       DASVJL010000138         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_20747, whole genome shotgun sequence.
ACCESSION   DASVJL010000138 DASVJL010000000
VERSION     DASVJL010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20747"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /gene="rmuC"
                     /locus_tag="VF357_02710"
     CDS             <1..308
                     /gene="rmuC"
                     /locus_tag="VF357_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX7523087.1"
                     /translation="VAYGWRQEQVAENAQEISRLGKEMYDRIGKFAEHFAHIRDGLDK
                     AVFAYNSAVGSYESRVLPGARRLRELRVTSDREEMKPPEIVDRATRPAPEGEDEPTS"
     gene            477..1250
                     /locus_tag="VF357_02715"
     CDS             477..1250
                     /locus_tag="VF357_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HEX7523088.1"
                     /translation="MLFRVAERVGGRALGATRGLGRTGVFLAAAVWNAVVPPYRGRDI
                     LKQIHFFGVKSLFVIVLTAAFTGMVLSLQGYYTLRKFGSEGLLGAAVALSLIRELGPV
                     LTALMVTGRAGSALAAEIGIMRIGEQIDSLETMGIDPVRFLVAPRIVAALVSIPLLTA
                     IFDVVGIYGGYLVGVKLLGLDAGQYFGHMKASVELADVVNGVWKSLAFSFLIVWVCSF
                     KGFFTERGAEGVSRATTSAVVISSVLVLIGDYVLTSVLM"
     gene            1247..>1935
                     /locus_tag="VF357_02720"
     CDS             1247..>1935
                     /locus_tag="VF357_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7523089.1"
                     /translation="MNCRDLSCEQCTVGDPEGPPILRLVGVDKILDGKKVLDHIDLDI
                     PRNRITAIIGLSGAGKSVLLKHMIGLLKPDRGQVLLDGVDLLHLSRKELFETRRRFGM
                     LFQSGALFDSLDVFGNVAFPLREKTKLGEEQIRERVLVLLRKVGLEKAGEKFPDELSG
                     GMVRRVALARAMVMDPEIILFDEPTTGLDPIIRNSILNLICRTFHDHKFTMVMISHDI
                     PDIFNWCHHVVV"
BASE COUNT          331 a          598 c          650 g          356 t
ORIGIN      
        1 ccgtcgccta cggctggcgc caggagcagg tcgcggaaaa cgcccaggag atcagccggc
       61 tggggaagga gatgtacgac cggatcggga agttcgccga gcatttcgcc cacatccgcg
      121 acgggctgga caaggcggtc ttcgcgtaca atagcgccgt ggggtcgtac gagtcgcgcg
      181 tcctcccggg ggcgcgccgg ctccgggagc tccgagtgac ctcggaccgc gaggagatga
      241 agccgccgga aatcgtcgac cgcgccaccc ggcccgcccc tgaaggcgag gacgaaccca
      301 cctcctgatc tggaaagtta tttttccctc gtctttttct ccgcgccgca cttccctcca
      361 tcgacattcg tatccgggat ccttccggcg gatgcgccaa ttcgcaacgg atgggcgccg
      421 gatgcaatct taggagggac aagatcgacc gtcggaatgg aaaaaagggg aagtggatgc
      481 tattccgcgt cgcggaaagg gtcgggggga gggcgcttgg ggcgacgcgc ggcctgggcc
      541 ggacaggggt cttcctcgcc gccgccgttt ggaacgcagt ggtacccccg taccgggggc
      601 gggacatcct gaagcagatc catttcttcg gcgtcaagtc gctcttcgtc atcgtgttga
      661 ccgccgcgtt cacgggtatg gtcctttcgc tgcaaggcta ctacaccctg cggaaattcg
      721 ggtccgaggg actgctgggc gccgcggtcg ccctgtcact gatccgggag ctgggtccgg
      781 tccttaccgc cttgatggtc acgggccgcg ccggctcggc cctggccgcg gagatcggga
      841 tcatgcggat cggcgaacag atcgactctc tggaaacgat ggggatcgac ccggtccggt
      901 tcctcgtggc gcccaggatc gtcgcggccc tggtctcgat ccccctgctc accgcgatct
      961 tcgacgtggt cggaatctac ggggggtatc tcgtcggcgt caagctcctg gggctcgacg
     1021 cgggccagta tttcggacac atgaaggcca gcgtggaact cgccgacgtg gtcaacggcg
     1081 tctggaagtc cctcgccttc tccttcctga tcgtctgggt gtgcagcttc aaggggttct
     1141 ttacggaacg gggggcggag ggagtgagcc gcgcgacgac gtctgcggtc gtcatctcct
     1201 cggtcctggt gctgatcggt gactacgtcc taacgtcggt gctgatgtga actgccgcga
     1261 tctctcctgc gaacagtgta ccgtcgggga tccggaggga cccccgatcc tgcgcctggt
     1321 gggcgtggac aagatcctgg acgggaagaa ggtgctcgac cacatcgacc tggacatccc
     1381 gcggaaccgg atcaccgcga tcatcggcct gagcggggcg gggaagagcg tgctgctcaa
     1441 gcacatgatc ggcctgttga agcccgaccg gggacaggtt ctcctcgacg gcgtggacct
     1501 gctccacctc tcccggaagg agctgttcga gacccgaagg agattcggca tgctcttcca
     1561 gtccggcgcg ctgttcgact ccctggacgt gttcgggaac gtggcgtttc ccctccggga
     1621 gaagacgaag ctgggcgagg agcagatccg ggagcgcgtc ctggtcctcc tccggaaagt
     1681 ggggctggaa aaggcggggg aaaagtttcc cgacgagctc agcgggggga tggtccggcg
     1741 cgtcgcgctg gcgcgggcca tggtgatgga ccccgaaatc atccttttcg acgaacccac
     1801 gaccgggctc gaccccatca tccggaactc catcctcaac ctgatctgca ggacgttcca
     1861 tgaccacaag ttcacgatgg tgatgatcag ccacgacatc ccggacatct tcaactggtg
     1921 ccaccacgtg gtggt
//
LOCUS       DASVJL010000139         8099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1428906, whole genome shotgun sequence.
ACCESSION   DASVJL010000139 DASVJL010000000
VERSION     DASVJL010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428906"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2622
                     /locus_tag="VF357_02725"
     CDS             <1..2622
                     /locus_tag="VF357_02725"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HEX7523090.1"
                     /translation="DAPGDTNYGKLGTGPTGTTWQYYNPDGQSTGFQTGQKARNSGSI
                     AWAKNLAVAATPIDNVQLFVVGVGMLSDGTGSDQPHLRVLRRVLRRMAEQQGTDLTTA
                     EYNSAAQSGDNFDGANIGAGKVFFADNADALLAALQQSFDSILLQSFSFTAPVVPAVR
                     TTDANRLYLASFLPDNPPETFWAGSCSSINLNSDGSVPANLAPATNWEAGDRLTSANR
                     IYSSNSRNVYTSSVSGTTWSRQDFFTGNTVSSYLTSTPSLLGLSTAADVNNVVDNVVM
                     RRLKTKRLGDIYHSTPVIIGSPSKFFNDTGFSTAVGSSPSFRDANATRQRVLYAGAND
                     GMLHAFNAGTFDNTSPPGSYNAGTGEELFAYVPKNLLPSLKNMTVTTTSTHQYMVDSS
                     PKAADVWLDTNGDNLKQTSEWKTVLIAGERKGGRGLFALDVTSPQSGTANYPKVMWEI
                     DNAAVPNLGQTWSEPNIGLVQIREGGVLKDRWVAFVGGGYWYSTTLKNNVSAGATSIN
                     IVDNTGFAPSGPSTLSIGTDNGISYGSTSATTIMGIPSSGSGSITTAHTAGEIVTSSP
                     IGRGFHVIDIKLGKVIWSWNTNMQYPITSSPTAVDINSDGRVNYVYVVDTGNQLWRFN
                     VGIAGTFDNTTNTIVNDNTWTATRIFSPSSPAIAQRSFNKVDVAFDQGFNTWVFFGTG
                     DREKPEDPGTGRIYAIRDDGSAPGTPYNESNLFNLTTVIANTDNTATGTILTGQKGWF
                     ADLPNTNEKMLSDVVMFNNNLFFTTFTSNTTNLCAGGGDARLYGINTGIAQAVAGSSM
                     TAGAGGLLPDSGTARIRSRILAGGGIPSSPVISMSASGGARLYVGTTNTASVLSFAIA
                     APTVFKRIKSWKEYIDQ"
     gene            2892..3596
                     /locus_tag="VF357_02730"
     CDS             2892..3596
                     /locus_tag="VF357_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523091.1"
                     /translation="MGRVHWYINGSEVAIENPQLPGTSYRRGDVVSAGAEIRRKEGEF
                     LLSAPAVTVRNALPDAIAADLSTLVPVAGDTVRVSASGKDPDGDGVTFRYHWYANDNL
                     VVGETSNSFKVMKGMKGMWIQAEVQAFDGIAEGSRLRTPKVRVLNAPPVVDHVEVKGD
                     GTKFTAIVRASDPDGDPLVLLGKSLPYGVTLIGNTLSWDAAAVPPGTEIPVVLRISDG
                     DGGEIEYSFRLSSAQK"
     gene            3640..4715
                     /gene="ispG"
                     /locus_tag="VF357_02735"
                     /gene_synonym="gcpE"
                     /pseudo
     CDS             3640..4715
                     /gene="ispG"
                     /locus_tag="VF357_02735"
                     /gene_synonym="gcpE"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
     gene            4712..6469
                     /locus_tag="VF357_02740"
     CDS             4712..6469
                     /locus_tag="VF357_02740"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX7523092.1"
                     /translation="MIRYSRYLMPTTKETPSDAEVVSHRLMLRAGLLRKVASGIYNYM
                     PAGLRVLRKVERILREEMDGAGALEVLMPAVVPSELWKESGRWDAYGKELLRFKDRAD
                     REFCLGPTHEEVVTDLVRREVRSYRQLPLTLYQIQDKFRDEIRPRFGLMRGREFFMKD
                     AYSFDADEEGAAESYRKMYDAYCRIFRRMGLDFRAVEADTGSIGGSSSHEFMVIADSG
                     EDAIVSCASCSYGANVEKAECFPSVAVTTPMETGGAKEGESKAGSPRKVSTPGKRTIE
                     EVSAFLAIDPATLIKTLLFETSVGDVAILVSGAHEVNEIKVKNHLGAEYVRLAGEERV
                     REMTGAPSGFAGPVGLSVRTIADQSVRSIASGATGANEKDAHLVDVVPGRDFIPEGYA
                     DLRVVREGDPCPRCGGTLRFSRGIEVGHVFRLGTKYSEAMRAVYLDEGGKERVTVMGC
                     YGIGVGRTAAAAIEQNHDDDGIVWPISIAPFEVAVIPVNMKQADVLAAAERVAADLDA
                     RGVEVFFDDREERPGIKFKDADLTGIPVRITLGEKNVTAGFGEIRDRKTGETVRIPLA
                     ELAKEAFALVEKKKAECAP"
     gene            6466..7176
                     /gene="pyrF"
                     /locus_tag="VF357_02745"
     CDS             6466..7176
                     /gene="pyrF"
                     /locus_tag="VF357_02745"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX7523093.1"
                     /translation="MKDRIIVALDTDSPESALATVKALSGEVGMFKVGMELFPRGGPE
                     LVRRIREAGFDVFLDLKFHDIPNTVAGAVRSAAALGVKFTTVHASGGRVMLTAAAEGV
                     RGTGTTILAVTVLTSLDDADLADVGFSLGAAEAVARLAALAVSCGIKGIVCSAKEVAS
                     VRARVGKGVTLVTPGVRMPEDAVGDQKRVVTPAEAIRRGADFLVVGRPITKAEDPAAA
                     ARAIAASMRGATTEGRRL"
     gene            7173..7928
                     /gene="rlmB"
                     /locus_tag="VF357_02750"
     CDS             7173..7928
                     /gene="rlmB"
                     /locus_tag="VF357_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX7523094.1"
                     /translation="MSGSMVGSVWVTGRHPVEELLASATQRARKVLLSDTVSREVRAG
                     FEKRAKDLALPCLTCPRQEWERRTGEHEGGGIAAEIAEYVYAEMEEWVSSLPGTARAF
                     LLDGITDPQNLGAIVRSARAFAFDGVVLPSDRSCPVTGAVFRASAGAAAHVPVLQVTN
                     LVRAIERLQEAGFWLYAAEGAEGTDLSAFAPAKRTAILLGSEEKGIRRLARERCDGVV
                     RIAMAPGAESLNVSVAAGIIAYSIRKSLTSPAG"
     gene            7968..8042
                     /locus_tag="VF357_02755"
     tRNA            7968..8042
                     /locus_tag="VF357_02755"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8000..8002,aa:Thr,seq:tgt)
BASE COUNT         1514 a         2510 c         2739 g         1336 t
ORIGIN      
        1 gacgccccag gggatacaaa ttacggaaaa ctcgggacag ggccgacagg gacgacctgg
       61 cagtattaca atccggacgg gcaatctact ggctttcaaa ctgggcagaa ggccaggaac
      121 tccgggagca tcgcctgggc aaaaaacctg gcagtggcgg cgacccccat cgacaacgtt
      181 caactcttcg tcgtcggcgt ggggatgttg agcgacggca ccgggagcga ccaaccgcac
      241 ctgcgcgttc tccgcagggt cctccgccgc atggcggagc agcaagggac cgaccttacc
      301 accgcggaat acaatagcgc ggcccaatcc ggggacaact tcgacggcgc gaacatcggg
      361 gcggggaagg tgttcttcgc cgataacgcc gacgccctcc tggccgccct ccagcagtcc
      421 ttcgattcga tcctcctgca gtcattctcc ttcaccgccc cggtcgttcc agcggtccgg
      481 accacggacg cgaaccggtt gtacctggcc tccttcctcc ccgacaatcc cccggagacc
      541 ttctgggccg gttcctgctc ctcgatcaac ctgaacagcg acggctcggt gcccgccaac
      601 ctcgcccctg ccaccaattg ggaagccggg gatcgtctga ccagcgcaaa ccgcatctac
      661 agcagcaact cccgcaatgt atatacgtca tccgtatccg gcaccacctg gtcgcgacag
      721 gactttttca ctggcaacac cgtatcgagt tacctcacat ccactccgtc ccttctcgga
      781 ctgtcgacgg ccgccgacgt gaacaatgtc gtcgacaacg tggtgatgag gagattgaaa
      841 accaagcgac tgggggacat ctaccactcg acccccgtca tcatcgggag ccccagcaag
      901 tttttcaacg acaccggctt ctccaccgcc gtggggtctt ccccgagttt ccgggacgca
      961 aacgccacgc gccagagggt cctctatgcc ggcgccaacg acggtatgct ccacgccttc
     1021 aacgcgggga cgttcgataa cacgtccccg cccggatcct acaacgcggg tacaggggag
     1081 gagttgttcg cctacgtccc gaaaaacctt ctcccgagtt taaaaaacat gaccgtcacc
     1141 accacttcga cccatcagta catggtcgac tcctccccga aggccgcgga tgtatggctg
     1201 gatacgaacg gcgacaacct caagcaaacc tccgaatgga agaccgtcct catcgccggg
     1261 gagcggaagg gaggccgggg gctgttcgcc ctcgacgtga cctcgccgca atcgggaaca
     1321 gcgaattacc ccaaggtgat gtgggaaatc gacaatgccg cggttcccaa tctcggtcag
     1381 acgtggtcgg agcccaacat cgggctcgtc cagatccgcg aaggcggcgt gctcaaggac
     1441 cgatgggttg ccttcgtggg gggcggctac tggtacagca cgacactgaa aaacaatgtt
     1501 tcggcaggcg cgacgtccat caacatcgtc gacaacaccg ggttcgctcc gtcgggcccg
     1561 agcacgctct cgatcggaac ggataatggc atttcctatg gctccacctc ggccacgacg
     1621 atcatgggca tcccctccag cggcagcggc agtatcacga ccgcccacac ggcgggagag
     1681 atcgtcacgt cttcccccat cggacgcggc ttccacgtga tcgacatcaa gctcgggaag
     1741 gtgatatgga gctggaacac caacatgcag tacccgatca cctcgtctcc cacggcggta
     1801 gacatcaaca gcgacggacg ggtgaactac gtctacgtgg tggacacggg caaccaactg
     1861 tggcgattca acgtggggat cgccgggacg ttcgacaaca ccaccaatac gatcgttaac
     1921 gataatacat ggaccgctac ccggattttc agcccgtcgt cgcccgccat cgcgcagagg
     1981 tcgttcaaca aggtggacgt ggcgttcgat caggggttca acacctgggt cttcttcgga
     2041 acgggagacc gggagaagcc cgaggatccg gggactggca ggatctacgc gatccgcgac
     2101 gacgggagcg caccgggaac tccatacaac gagtcgaacc tgttcaacct cactacggtg
     2161 atcgccaaca ccgacaatac cgcgacgggg acgatcctga ccggccagaa aggctggttc
     2221 gccgacctcc cgaacaccaa cgagaaaatg ctctccgacg tggtgatgtt caataacaac
     2281 ctgttcttca cgacctttac ctccaatacg acgaaccttt gcgccggggg cggggacgca
     2341 cgactgtacg ggatcaatac cggcattgca caggctgtcg ccgggagcag catgacggcg
     2401 ggggcggggg gcctcctgcc cgactccgga accgcccgga ttcgcagcag gatcctggcc
     2461 ggcggaggaa tccccagcag cccggtgatc tcaatgtcag ccagcggtgg tgcacgcctc
     2521 tacgtgggga cgacgaacac ggcctctgtt ctgtcctttg ccatcgccgc cccaaccgtg
     2581 tttaaaagga tcaagagctg gaaggagtac atcgaccaat gaaccttcgg acggccgctt
     2641 tcatcgcagc cgccgtgctc ctctccgcga tcggttgcgg cggagggggg gggggcggta
     2701 gcccgaaagg aggccaaccg ggatcggtga ttcctccggc gggttccacc ggaccgcaag
     2761 acgcatcgaa aagcgtcgtt tccaacacga tcccgcaagg cttcgccctg cgcctcatcc
     2821 cggagaatcc ggacgtattg tcaggaattc gtgcctccct ttccattccg ggagggaggg
     2881 ataaagcgac gatgggacgg gttcattggt acatcaacgg gagtgaggtg gccatagaga
     2941 atccgcaact gcccgggact tcgtaccggc gtggcgacgt cgtctccgcc ggtgctgaaa
     3001 tccgcagaaa agaaggggag ttccttcttt ccgccccggc ggtcaccgtc cggaacgccc
     3061 tcccggatgc gattgccgcg gacctttcga cgctggtccc ggtagcgggg gacaccgtcc
     3121 gggtttccgc gtcggggaaa gatcccgatg gcgatggggt gaccttccgg taccattggt
     3181 acgcgaacga caatcttgtg gttggggaaa caagtaattc cttcaaggta atgaaaggaa
     3241 tgaaggggat gtggatccag gcggaggttc aggcattcga cgggatcgcg gagggctccc
     3301 ggctgcggac cccgaaggta cgggtgttga acgccccgcc ggtcgtggat catgtggagg
     3361 tcaagggcga cgggacgaag ttcaccgcca ttgtgcgcgc ttcggatccc gacggggacc
     3421 ccctcgtcct ccttgggaaa tccctaccct acggagtgac tctgatcggg aacacattga
     3481 gttgggacgc ggctgccgtg cccccgggga cggaaatccc cgtcgttctc cggatctcgg
     3541 atggagacgg gggagagatc gagtactctt tccggctctc ctcggctcaa aaatagcgtc
     3601 gacgcttaag ccgcttctcg catatagtaa tcccccacca tgaagacacg aagcaagacc
     3661 aagcgcatcc gcgtcggcgg cgtgccggtg ggcgggggcg cccccgtctc cgtccaaagc
     3721 atgacgaaca ccgacacccg cgacgtccgg gcgaccgttg cgcagatccg gtcgctcgcg
     3781 agggaagggt gcgagatcgc gcgcgtcgcc gtgccggacg cgaaggcggc ggcggcgttc
     3841 gggaaaatcc gcgcagggtc gccgatcccg gtcatcgccg acatccactt cgaccaccgg
     3901 ctcgcgatcg cctgcgccga cgcgggggcg gattgcctgc ggatcaaccc cggcaacgtc
     3961 ggtggggaga agaacacgat cgaggtcctg cgggcggcgc gggcgaaccg gatcccggtg
     4021 cggatcggcg tcaacgcggg gtccgtcgag aaagatcttc tcgcgaaaca cggagggccg
     4081 acgccgaaag cgctcgttgc gagcgccgca cgatacctga agattagtga aaaggcgcgc
     4141 ttcacggcgc tcaaattctc cctcaaggcg tccgacgtgg tcacgacgat cgaggcgtac
     4201 cgcctcttct cgcgccggta cgattttccg ctgcacatcg gggtcacgga ggcggggacg
     4261 atcttttcgg ggacgatcaa gtcgtccgtc gggatcggcg ttcttctcgc ggaggggatc
     4321 ggcgatacgt tgcgggtgtc gctcacaggg cctccggagg aagaagtccg cgtcggctgg
     4381 cagatcctga aaagtctcgg gctgcggcat cgggcccgga gttcatctca tgccccacct
     4441 gcggccgggt gtcgatcgat atcgtgagga tcgcgacgga ggtggagcgg cggctgtcgc
     4501 acctgaccgt tcccctgaag gtcgccgtaa tggggtgcgc cgtcaacggt cccggcgagg
     4561 cgcgcgaggc cgacgtgggg gtggcgggcg gcaagggggt agggatcatc ttcgtgaagg
     4621 ggaagatcgt gaagaaggtg aaggagaccg agatcgcggc cgaagtcgtc cgaatcgcgg
     4681 aaatgctggc tgtcgaaaaa aggagaagca agtgatccgg tattcccgct atctgatgcc
     4741 gaccaccaag gagacgccgt ccgacgcgga ggtcgtcagc caccggctga tgctgcgcgc
     4801 ggggctcctc cggaaagtgg cctcggggat ctacaactat atgcccgccg gtctgcgcgt
     4861 cctgcgcaag gtggagcgga tcctgcgcga ggagatggac ggggcggggg cgctggaggt
     4921 gctgatgccc gcggtggtgc cgtccgaact gtggaaggag agcggccggt gggacgccta
     4981 cgggaaggag ttgctccgtt tcaaggaccg ggcggaccgt gagttctgcc tgggccccac
     5041 ccacgaagag gtggtcaccg acctcgtgcg gcgggaagtc cggtcgtacc ggcagctgcc
     5101 gctcaccctc taccagatcc aggacaagtt ccgtgacgag atccgccccc gcttcgggct
     5161 gatgcgtggc cgggagttct tcatgaagga cgcctactcg ttcgacgcgg acgaggaggg
     5221 ggcggcggag tcgtaccgga agatgtacga cgcctattgc cggatcttcc gccggatggg
     5281 actcgatttc cgcgccgtcg aggcggatac cgggagcatc ggcggctcga gctcgcacga
     5341 gttcatggtg atcgccgact ccggcgagga cgcgatcgtc tcctgcgcct cctgttccta
     5401 cggggcgaac gtcgagaagg cggaatgctt tccctccgta gctgtgacca cgcctatgga
     5461 aacgggagga gcgaaggagg gggaatcgaa ggcaggttcg cctcgcaaag tttccacccc
     5521 cggcaagcgg acgatcgagg aggtgtcggc cttcctcgcg atcgatcccg cgacgctcat
     5581 caagacgctc ctgttcgaaa cgtccgtcgg ggacgtggcc atcctcgtct ccggggccca
     5641 cgaagtgaac gagatcaagg tgaagaacca tctcggcgcc gaatacgtcc ggctggccgg
     5701 ggaggagcgc gtgcgggaga tgacgggcgc cccgtcgggg ttcgccgggc ccgtcgggct
     5761 ctccgtgcgt acgatcgcgg accaatcggt gcggtcgatc gcctcggggg ccaccggcgc
     5821 gaacgaaaag gacgcccacc tcgtcgatgt cgtccccggg agggatttca ttccggaagg
     5881 gtacgcggac ctgcgggtgg tgcgcgaggg ggacccgtgc ccgcggtgcg gcgggacgct
     5941 gcggttctcg cgcgggatcg aggtggggca cgtcttccgg ctcggcacga aatacagcga
     6001 ggcgatgcgg gcggtctacc tcgacgaggg cggcaaggag cgggtgaccg tgatggggtg
     6061 ttacggcatc ggcgtcgggc ggaccgcggc ggcggcgatc gagcagaacc acgacgacga
     6121 cgggatcgtc tggccgatct ccatcgcccc gttcgaggtc gccgtcatcc ccgtgaacat
     6181 gaagcaggcc gacgtgttgg cggcagcgga gcgcgtggcg gcggatctcg acgcacgcgg
     6241 cgtggaggtt ttcttcgacg atcgcgagga gcgccccggc atcaagttca aggacgcgga
     6301 tctgacggga atccccgtgc gcatcaccct cggggaaaag aacgtgacgg ccgggttcgg
     6361 cgagatccgc gaccggaaga cgggggagac ggtccggatc ccgctggcgg aactggcgaa
     6421 agaggccttc gcgctggttg agaagaaaaa agcggagtgc gccccgtgaa ggaccggatc
     6481 atcgtcgccc tcgacaccga ctcgcccgag tccgccctgg cgacggtcaa ggccctttcg
     6541 ggcgaggtcg ggatgttcaa ggtggggatg gaactttttc cccggggtgg ccccgaactg
     6601 gtccggcgca tccgcgaggc cgggttcgac gtctttctcg atctgaaatt ccacgacatc
     6661 cccaacacgg tcgcaggcgc cgtccgctcc gccgcggcgc tgggggtgaa attcaccacg
     6721 gtccacgcct cgggcggtcg ggtgatgctc acggcggccg cggaaggcgt tcgcggcacc
     6781 gggacgacca tcctcgccgt gaccgtcctc accagcctgg acgacgcgga tctggcggac
     6841 gtcggattct ccctcggcgc ggcggaggcg gtggcgcgcc tcgccgccct cgcggtttcc
     6901 tgcgggatca aagggatcgt ctgctccgcg aaggaagtgg cgtccgtgcg tgcccgcgtc
     6961 gggaaaggag tcaccctcgt cacccccggc gtgcggatgc cggaggacgc cgtcggggac
     7021 cagaagcggg tggtgacccc tgccgaggcg atccggcggg gcgcggattt cctggtcgtc
     7081 ggacggccga tcacgaaggc ggaggatccg gcggcggcgg cgcgggcgat cgcggcgtcg
     7141 atgcgcggcg ccaccacgga agggagaagg ttatgagcgg ctcgatggtc ggttccgtgt
     7201 gggtgacggg ccggcatccc gtcgaggaac tcctcgcatc ggcgacgcag cgggcccgga
     7261 aggtcctctt gagcgacacg gtctccaggg aggtgcgggc gggattcgag aagcgggcga
     7321 aggatctcgc gctgccgtgc ctcacctgtc cacgccagga atgggagcgg cggacggggg
     7381 agcacgaggg gggcgggatc gccgcggaga tcgcggagta tgtatatgcg gagatggagg
     7441 agtgggtctc ctcgttgccc gggacggcga gggcattcct gctcgacggg attaccgacc
     7501 ctcagaacct cggggcgatc gtgcgcagcg cgcgggcgtt cgccttcgac ggcgtagtcc
     7561 ttccctccga ccggtcgtgc ccggtgaccg gtgcggtctt ccgcgcgtcc gccggcgccg
     7621 ccgcgcacgt accggtgctc caggtgacga accttgtccg cgccatcgaa cgcctgcagg
     7681 aggccgggtt ctggctctac gcggcggaag gagctgaagg gacggatctg tcggcgttcg
     7741 ctcccgcgaa gcgcacggcg atcctcctcg ggagcgagga gaaggggatt cgccggctgg
     7801 cgcgggagcg gtgcgacggc gtcgtccgga tcgcgatggc ccccggggcc gagtccctga
     7861 acgtctccgt cgccgcgggg atcatcgctt attccatcag aaaatcgttg acatctcccg
     7921 ccggatagga tcaaattttc tgtttctgga tgtcggtaga ggtgcgcgcc gacgtagctc
     7981 aacggtagag cgtctgattt gtaatcagat ggttggaggt tcaactcctc tcgtcggctc
     8041 caaccgtgaa gtggaggggt acccgagcgg ccaaaggggg cagactgtaa atctgctgg
//
LOCUS       DASVJL010000140         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_209139, whole genome shotgun sequence.
ACCESSION   DASVJL010000140 DASVJL010000000
VERSION     DASVJL010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209139"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF357_02760"
     CDS             <1..216
                     /locus_tag="VF357_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523095.1"
                     /translation="PEQEQRLVPGTPGWDFLPPRIFPRDGEATQAMVRNAIVYACHHP
                     KWGGRKPSVAFIVEGPYAIPCVQGILP"
     gene            221..1579
                     /locus_tag="VF357_02765"
     CDS             221..1579
                     /locus_tag="VF357_02765"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX7523096.1"
                     /translation="MPPSDKDRLSRCFRAAVEAVDPARLVASALRVEGDAVVLDAAGV
                     RAAIPLSSLWKIYLVGAGKAGRAMGEAALAALGKRVAGGVIAVPRGAEGQSSTVRFAA
                     AGHPVPDIFSLGAAREILSLLEFAGEGDLVIGLVSGGGSAMLSAPVAGITMEEKADTF
                     RHLLRAGADIASFNAVRKHLSEIKGGLLARAAQPATTWALLLSDVPGDDPSVIASGPF
                     SPDPTTFADAIGVLERYGIFYAVPYAVRRHLAEGAAGSLPETPKSDDPAFLGTISALL
                     GANRTAIDAAALRMARERDTVPTAIVLLPDFLHGEARECARSFCARLRKAAEALSPGH
                     AVAMIAGGETTVNVRGRGKGGRNQEFALAAAVELSGEEAMAVLAAGTDGIDGPTDAAG
                     AYADGSTVARASSLGLDPDAHLSRNDAYPFFEALGDLVVTGPTGTNVADLVIGIATTV
                     TR"
     gene            1606..>1910
                     /locus_tag="VF357_02770"
     CDS             1606..>1910
                     /locus_tag="VF357_02770"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7523097.1"
                     /translation="MKNISPETTHVGWIGTGVMGLSMCGHLLAKGYDVTVYSRSKEKA
                     RPLLVKGAKWADSPGAVAASSDVVFSMVGFPADVREVYLGPNGVLASSRAGTIVVDMT
                     "
BASE COUNT          290 a          620 c          685 g          315 t
ORIGIN      
        1 ccggaacagg agcagcggct ggttcccggc accccgggtt gggacttcct gccgccccgc
       61 atcttcccgc gggacgggga ggcgacgcag gcgatggtcc ggaatgccat tgtgtacgca
      121 tgccatcacc cgaaatgggg aggcaggaag ccgtcggtcg cttttatcgt ggaggggccg
      181 tacgcgatcc cttgcgtcca agggatcctc ccctagggag atgcctccct cggacaagga
      241 tcggctgtcc cggtgcttcc gtgccgccgt ggaagcggtc gacccggcgc gcctcgtcgc
      301 atccgcgctg cgcgtggaag gggatgccgt cgtgctcgat gccgcgggcg tcagggcggc
      361 gatccccctt tcgtccctgt ggaagatcta cctcgtcggg gccggaaagg cgggaagggc
      421 gatgggagag gcggctctcg cggcactggg gaaacgcgtc gcgggcgggg tgatcgccgt
      481 gccacgcggc gcggaagggc agtcaagcac ggtgcgattc gcggcggccg ggcacccggt
      541 ccccgacatc ttcagcctcg gggcggcgcg cgagatcctc tcgcttctgg aattcgccgg
      601 ggaaggggac ctcgtgatcg gtctcgtctc gggcggcggt tccgccatgc tgtccgcgcc
      661 ggtcgcaggg atcacgatgg aagagaaggc cgatacgttc cgtcatctcc tgcgtgcggg
      721 ggccgacatc gcgtcgttca acgcggtgcg caagcatctc tccgagatca agggggggct
      781 gctcgccagg gcggcgcagc ctgcgaccac atgggcgctg ctcctgtcgg atgttcccgg
      841 cgacgacccg tccgtgatcg cctccggacc tttttccccc gacccgacga cgtttgcgga
      901 cgcgatcggg gtcctcgagc ggtacggtat cttttacgcg gttccctacg cggtgcgtcg
      961 acaccttgcc gaaggcgcgg ccggctctct ccccgaaacc ccgaaatcgg acgaccccgc
     1021 tttcctcgga accatctcgg cgcttctcgg ggcgaaccgg acggcgatcg acgcggcggc
     1081 gctgcggatg gcgcgggagc gggacacggt ccccaccgcg atcgtcctcc tgcccgattt
     1141 ccttcacgga gaggccaggg agtgcgcccg ttcgttctgt gcccggttgc ggaaagccgc
     1201 ggaggcgctt tccccaggac atgcggtcgc gatgatcgcc ggcggagaga cgacggtgaa
     1261 cgtgcgcggt agaggcaagg gagggcggaa ccaggagttc gcgctcgccg cggcggtgga
     1321 actgtccggc gaggaagcga tggccgtcct ggccgcgggg acggacggta tcgacgggcc
     1381 caccgacgcc gcgggggcgt atgccgacgg atccaccgtt gcgcgcgcct cgtcccttgg
     1441 gctcgacccc gacgcgcatc tgtctcgcaa cgacgcgtac ccgttcttcg aggcgttggg
     1501 agacctggtc gtcaccggac caacgggcac caatgtcgcc gacctggtga tcggtatcgc
     1561 cacgaccgtg acacggtaac cttcaagaca agggggcctg cgaagatgaa gaacatatca
     1621 ccggaaacga cgcacgtcgg gtggatcggt accggggtga tggggctgtc gatgtgcgga
     1681 caccttctcg cgaaggggta cgacgtcacc gtgtactccc gctcgaagga gaaggcccgc
     1741 cccctcctcg tgaagggagc gaaatgggcg gactcccccg gcgccgtcgc ggcatcttcc
     1801 gatgtggtgt tcagcatggt cggctttccc gcggacgtgc gggaggttta cctcggaccg
     1861 aacggcgtgt tggcctcctc cagggccggg accatcgtcg tggacatgac
//
LOCUS       DASVJL010000141         4040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1334008, whole genome shotgun sequence.
ACCESSION   DASVJL010000141 DASVJL010000000
VERSION     DASVJL010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334008"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VF357_02775"
     CDS             <1..500
                     /locus_tag="VF357_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX7523098.1"
                     /translation="TMPYWDERFWSLAKEYPDIEADQFHIDALVARFVTHPQHFDVVV
                     GSNLFGDILSDLGPAIAGSIGIAPSANLNPEKKHPSMFEPVHGSAPDITGKGIANPMG
                     QIWCGAMMLEHLGFPESGDLVMEAMKSVLAAGICTADIGGKAKTAEVTDAICAKIRKI
                     GLKAR"
     gene            524..1609
                     /locus_tag="VF357_02780"
     CDS             524..1609
                     /locus_tag="VF357_02780"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: protein motif:HMM:TIGR02089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX7523099.1"
                     /translation="MRTHKIAVIPADGIGKEVIPAGVRVLQELSERDGAFRFQFKTFP
                     WGSDYYHEHGHMMPADGLDTLRRFDAIFFGAVGHPSIPDHITLWGLRIPICQGFDQYG
                     NYRPARILPGITGPLRGRTGKDLDWIVVRENTEGEYSGVGGRVHKGLPGEVAMEVSVF
                     TRAGVERIMRFAFVLARKRPRRLLTLVTKSNAQRHGMVMWDEIFAEVAREFPEVATDR
                     MLVDAVTTRMVLKPETLDVLVASNLHADILSDLAAALSGSLGIGPTANINPERTYPSM
                     FEPIHGSAFDIMGKGIANPLGTFWTAVLMLEHLGEEVAAANLMKAIEQVTAAGKDLPR
                     DLGGNASTEQVTDAVCARIRKIGRKTR"
     gene            complement(1557..2150)
                     /locus_tag="VF357_02785"
     CDS             complement(1557..2150)
                     /locus_tag="VF357_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HEX7523100.1"
                     /translation="MRPRRFLLKPFPGEGNPAGLTIGGSIGRRADTLSVLYEVRGNLS
                     KVSIPTAAEAPRRKGRLWEETCLELFLGTEDSGGYWEFNLSPAGHWNVYRFTSYRKDM
                     VEEPAITSLPIDVRRDSEALLLAAEIGVGKIVPAEKDLAATVAAVIGTIDGENSHWAP
                     VHPASRPDFHRRDGFALTLPAIGSCARSCGSSRRRHR"
     gene            complement(2160..3317)
                     /locus_tag="VF357_02790"
     CDS             complement(2160..3317)
                     /locus_tag="VF357_02790"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX7523101.1"
                     /translation="MLVEKRFAPLRGRAVGLVCNPTAVDRYLRHAADLLHEARGVRLA
                     ALFGPEHGVRGEIQYMAAARGERDRKIGVPVHSLYGNGAGSLRPAGKALRGLDALIFD
                     IQDVGARYYTYQATMLFCMEAAAHAKLDFFVLDRPNPIGGRHVEGPALRPGFGSFCGV
                     HDVAVRHALTVGELAALYRQERKLDLALTVVPCRGWRRKMFQRDTRLPWVFPSPNMPT
                     PETALVYPGMCLLEGTNLSEGRGTTRPFELFGAPWLDADSLSEALSAERLPGVRFRPV
                     RFVPTWDKHAGKRCHGVEVFVVDREAFRPFRTGVACVAAARAQDPERFRWRREAYEFV
                     EGIPAFDLLCGSPREREAIERGLGWRDLAADWSREERAYARRRALHLRYDT"
     gene            3592..3774
                     /locus_tag="VF357_02795"
     CDS             3592..3774
                     /locus_tag="VF357_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523102.1"
                     /translation="MARLFAVYKVENGTRLLLGGLAECRKSRRKESNKRALVEMAKRK
                     YAGSPTEELRVVLEEE"
BASE COUNT          766 a         1292 c         1318 g          664 t
ORIGIN      
        1 tcacgatgcc gtactgggac gagaggttct ggtccctggc gaaggagtat ccggacatcg
       61 aggccgatca gttccacatc gacgccctcg tcgcgcggtt cgtcacccac ccgcagcatt
      121 tcgacgtcgt ggtgggaagc aacctgttcg gcgacatcct ctccgacctg ggccccgcca
      181 tcgcgggaag catcgggatc gcaccctccg cgaacctgaa cccggaaaag aagcacccct
      241 ccatgttcga gcccgtgcac ggctccgcgc ccgacatcac gggcaagggg atcgccaacc
      301 ccatgggcca gatctggtgc ggcgccatga tgctcgagca cctcgggttc cccgagtcgg
      361 gcgacctcgt catggaggcg atgaagtccg tcctcgcggc agggatctgc acggcggata
      421 tcggagggaa ggcgaaaacc gccgaggtga cggacgccat ctgcgcgaag atccgcaaga
      481 tcgggctcaa ggcccggtag aaggaaacga cggagggaaa ggcatgcgca cccacaagat
      541 cgcagtgatc ccggccgacg gcatcgggaa agaggtcatc cccgccgggg tccgcgttct
      601 ccaggagctc tcggaacgcg acggcgcatt ccgcttccag ttcaagacgt tcccgtgggg
      661 gagcgactac tatcacgagc acggacacat gatgcccgcc gacggcctgg acaccttgcg
      721 ccggttcgac gcgatcttct tcggtgccgt cgggcacccg tccatcccgg accacatcac
      781 gctgtggggg ctgcgcattc ccatctgcca ggggttcgac cagtacggga actaccggcc
      841 cgcgcgcatc cttcccggga tcacggggcc gctccggggg cgcaccggca aggacctgga
      901 ctggatcgtc gtccgggaga atacggaagg ggaatattcc ggcgtcggag gaagggtcca
      961 caagggtctg cccggagagg tggcgatgga ggtgtcggtg ttcacacgtg ccggggtgga
     1021 gcgcatcatg cggttcgcct tcgttctggc gcggaagcgg ccccggcggt tgctgacgct
     1081 ggtgacgaaa tcgaacgcgc agcgccacgg gatggtcatg tgggatgaga tctttgccga
     1141 ggtcgcccgg gagtttccgg aggtggcgac cgaccggatg ctcgtggacg cggtcaccac
     1201 gagaatggtg ctcaagcccg agaccctgga cgtgctcgtc gcctccaacc tgcacgcgga
     1261 catcctctcc gacctcgcgg cggcgctgtc gggaagcctc gggatcggcc cgaccgcgaa
     1321 catcaacccg gaaaggacgt acccgtcgat gttcgaaccg atccacgggt ccgccttcga
     1381 catcatggga aaagggatcg ccaatcccct cgggacgttc tggaccgcgg tccttatgct
     1441 ggagcaccta ggggaggagg tcgccgccgc gaacctcatg aaggccatcg agcaggtcac
     1501 cgccgccggg aaggaccttc ccagggacct gggcgggaac gcctcgacgg agcaggtcac
     1561 cgatgccgtc tgcgcgagga tccgcaagat cgggcgcaag acccgatagc ggggagggtc
     1621 aatgcgaacc cgtcccttcg gtgaaagtcc ggtcgggagg cgggatggac cggcgcccag
     1681 tggctgttct ctccatcgat cgtcccgatc acggcggcga ccgtggccgc aaggtccttt
     1741 tctgccggga cgatcttccc gacgccgatc tccgcggcga gaaggagtgc ctccgaatcc
     1801 cttcggacgt cgatcggcaa tgacgtgatg gccggctcct ccaccatgtc cttccggtat
     1861 gacgtgaacc gatagacgtt ccagtgcccc gccggcgaga ggttgaattc ccaatagccc
     1921 ccggaatcct cggtgccgag aaacagttcg aggcaggttt cctcccacag tcggcccttt
     1981 cttcggggcg cctccgcggc ggtagggatc gacaccttcg acaggttccc ccgcacctcg
     2041 tagaggaccg acaaggtatc cgcgcgacgc ccgatcgatc ccccgatcgt caggccggcc
     2101 gggtttcctt ccccggggaa cggcttcagg agaaatcgcc gcggacgcat cggacgggat
     2161 tacgtatcgt accggaggtg gagcgccctt ctcctggcgt acgcccgctc ctcgcgcgac
     2221 caatccgccg cgaggtcgcg ccaccccagg ccccgttcga tcgcctcccg ctcgcggggc
     2281 gatccgcaca gcaggtcgaa cgccgggatc ccctcgacga attcgtacgc ttctctccgc
     2341 caccggaagc gttccggatc ctgcgcgcgc gccgcggcga cgcacgccac gccggtacgg
     2401 aagggacgaa acgcctcccg gtcgacgacg aagacctcga cgccgtgaca acgctttccg
     2461 gcatgcttgt cccacgtcgg gacgaagcgg accggccgga accggacccc cggcagacgt
     2521 tcggccgaga gcgcttccga aagggagtcg gcgtcgagcc agggcgcgcc gaacagttcg
     2581 aacggcctcg tggtgccccg tccctcgctc aagttcgtcc cttccaggag gcacatcccg
     2641 ggatagacga gcgccgtctc cggcgtcggc atgttgggcg acggaaagac ccacggcagc
     2701 cgggtgtccc gctggaacat tttccgccgc cagccgcggc agggaaccac cgtgagcgcg
     2761 aggtcgagtt tccgctcctg ccggtagagg gccgccagtt cgccgacggt gagggcgtgg
     2821 cggacggcca cgtcgtgcac gccgcaaaaa ctcccgaagc cggggcgaag cgccggcccc
     2881 tccacgtggc ggccgccgat cgggttcggc cggtcgagaa cgaagaagtc gagcttcgca
     2941 tgcgcggcgg cctccatgca gaagagcatg gtggcctggt acgtgtaata gcgcgcgccg
     3001 acgtcctgga tgtcgaagat cagcgcgtcg agcccgcgaa gcgccttgcc cgccggccga
     3061 agcgacccgg cgccgtttcc gtacaacgag tgaaccggaa ctccgatctt ccggtcgcgt
     3121 tcgccgcgcg cggcggccat gtactggatc tccccgcgga cgccgtgttc cgggccgaag
     3181 agggcggcaa gccggacgcc ccgcgcctcg tggagcaggt ccgcggcgtg acgcaggtac
     3241 cggtccactg ccgtggggtt gcagaccaac ccgacggcgc gtccccgcag gggggcgaag
     3301 cgtttttcga cgagcacgtc gaggccggtc cagactttct ccgtgcgacg cccggcgacc
     3361 gggcctgcgt ctcccataac cggtgaagat accacatctc cccggggatt ccgcccctca
     3421 gggaaacctg ttgaaaagat tttgataatt gggtaatccg tttcctgaat ttcatggccg
     3481 cataagcgca tgtacctata aataaaggag atctatttcc ggcatcgatt ttgctttcta
     3541 cttggtgcga cccgggagga gtcgaaacag ggaggagagg aggcgggcga attggcgcgg
     3601 cttttcgcgg tctacaaagt ggaaaacgga acgaggctcc tcctgggcgg cctggcggag
     3661 tgtcgaaaat ccaggcgcaa ggaatccaac aagcgcgctc ttgtggagat ggcaaagcgg
     3721 aaatacgcgg ggtcgcccac cgaggaattg agggtcgtcc tggaagagga gtgacctccc
     3781 tttccgtcgc gtggacggaa tgttcgctcc ccctaccgtg caaggaaaaa atcgaaaaaa
     3841 cgtaatatga tttaagtata cgaaaataaa tgaatatttg tattgtatac ttcatatgtc
     3901 atgaaattta ggaaacggat tacccaatta tcaaaatctt tttaacaggt ttccctgagg
     3961 ggcggaatcc ccggggagat gtggtatctt caccggttat gggagacgca ggcccggtcg
     4021 ccgggcgtcg cacggagaaa
//
LOCUS       DASVJL010000142         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_116054, whole genome shotgun sequence.
ACCESSION   DASVJL010000142 DASVJL010000000
VERSION     DASVJL010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116054"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VF357_02800"
     CDS             complement(<1..486)
                     /locus_tag="VF357_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7523103.1"
                     /translation="MRERNAICAVIPAYNAERTVGAVVRGALSFLTTVIVADDGSTDE
                     TAQVAEDAGALVIREPDNHGKGHCLRLLFAEARNRGYDAVIALDSDGQHDPADIPRFL
                     EAHRKDPGSVIVGSRMADEEGIPVHRKNSMLVARFYVCLAANRYIDDTQCGYRLYPLS
                     VV"
     gene            complement(515..2332)
                     /locus_tag="VF357_02805"
     CDS             complement(515..2332)
                     /locus_tag="VF357_02805"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX7523104.1"
                     /translation="MAGIAAKGSEGSGAGIKVGLAGEYASAAEANGVIWQDMVLSFLS
                     SFVLVLVLVYAAYRPPVAVLCAFVLTLFAALSWTLLFAYLLYGGLNIVTSIVAAMLIG
                     LYVDYMIMVYHRFHGEIRRGRSPLQALETTFSETGKALLSSSATSCVAFFSVVVTSFR
                     GLHELGVVAGFGILFCLLSTFLVMGSLLSWLAKVSPTRIEARRPGGVPTGWAERLVEG
                     RSGTVVAVFSIFLFLSILGSARTRFDAGIEAIGPVDSKVEQVQRVIEEKFGRKGEPLF
                     LVARADGDRRLAADFDALDLQGERWRRAGRVETFSSPATLVPPPRFQKESRRLLSGAG
                     LPGRYDDAGLERAIRREMEAQGMVPGDGLAAYAAGIVNVLAGEGIVDLPGFARSGDPR
                     ATYFFNGSRNAIAAHLSPPGGRWGQGALAAMKEDVLALGPDFALTGPSLIFEEIRSSI
                     VRESTLATFIAFAANWIIVWLHFRRLRDTALVMLPVTAGSLLTVGAMGAIGIPFNFFN
                     VAGIALLFGFGVDYGIYYMQSRRESPAAGAAEALRRTGGGIALCALTTLASCGSIILS
                     HYRGLASIGAVLCLGAVFCLLSTVLLLPSLIDALERPRT"
BASE COUNT          462 a          808 c          723 g          348 t
ORIGIN      
        1 caccacggac agcgggtaaa gacgataccc gcattgggta tcgtcgatgt agcggttggc
       61 cgcgaggcag acgtagaatc tggcgaccag catcgaattt ttccggtgaa cggggatccc
      121 ttcttcatcg gccatcctcg aaccgacgat gacggatccg gggtctttcc gatgggcttc
      181 gagaaaccgc gggatgtccg ccggatcgtg ctgtccatcc gaatccagcg cgatgacggc
      241 gtcgtatccc cggttacggg cttccgcgaa cagcaggcgc agacagtggc ccttcccgtg
      301 gttatccggt tcccggatga ccagcgcacc cgcgtcctcc gcgacctggg cggtttcgtc
      361 ggtagaaccg tcgtcggcaa cgatgaccgt cgtcaggaag gaaagcgccc ctcgtacaac
      421 cgcccccacc gtccgttccg cattgtaggc agggatgacg gcgcatatcg cattgcgctc
      481 cctcattccg catccttaag acccgccgcg ggtttcacgt tcgcgggcgc tcgagcgcat
      541 cgatcaggga aggaagcagc aggaccgtcg agagaaggca gaagaccgcc ccgaggcaca
      601 gcacggcgcc gatggacgcc agcccccggt aatgagagag gatgatgctt ccacaggagg
      661 ccagcgtggt gagcgcgcag agggcgatac cgcccccggt ccggcgaagc gcctccgcgg
      721 ctcccgccgc ggggctttcc ctgcgggact gcatgtaata gatcccatag tcgaccccga
      781 acccgaaaag gagtgcgatc cccgccacgt tgaaaaagtt gaaggggatc ccgatcgctc
      841 ccatggcgcc cacggtgagg agcgatcccg ccgtcaccgg cagcatcacg agcgccgtat
      901 cccgcaggcg acggaagtgc aaccacacga tgatccagtt cgccgcgaag gcgatgaacg
      961 tggcgagggt gctttcccgc acgatcgagg accggatctc ctcgaaaatg agggacgggc
     1021 ccgtcagcgc gaagtccggc cccagggcca gaacgtcttc cttcattgcg gcgagcgccc
     1081 cttgccccca ccgtccgccg ggcggcgaca ggtgtgcggc gatcgcattg cgggacccgt
     1141 tgaagaaata cgtcgcccgg gggtctcccg agcgggcaaa cccgggcagg tcgacgatcc
     1201 cttccccagc gaggacgttg acgatcccgg cggcatacgc cgcaaggccg tcccccggta
     1261 ccatcccctg cgcctccatc tcccgccgga tcgccctctc gagacccgca tcgtcgtacc
     1321 gccccggcag cccggcgccg gacagaagtc tccgcgactc cttctggaaa cggggagggg
     1381 gtaccagcgt cgccggggaa gagaacgttt ccactctccc cgcccgcctc catcgctccc
     1441 cctggagatc gagggcgtcg aaatccgcgg cgagccgccg gtcgccgtcc gcacgcgcca
     1501 cgagaaagag cggttcccct ttcctcccga atttctcctc gatcacgcgc tggacctgct
     1561 ccaccttgct gtccacgggg ccgatcgcct cgatccccgc gtcgaaccgg gtccgcgccg
     1621 atccgaggat cgacaggaag aggaagatcg aaaaaaccgc cacgacggtt ccgctcctcc
     1681 cttcgaccag ccgttccgcc cagcccgtcg gaacgccccc gggtctcctc gcctcgatcc
     1741 gcgtcgggga taccttcgcg agccaggata ggagggatcc catcaccaga aacgtggaca
     1801 ggaggcagaa caggatcccg aatccggcca cgacccccag ttcgtggagc ccgcgaaaac
     1861 tggtcacgac cacggagaag aaggcgacgc aactggtcgc ggaactgctg aggagcgcct
     1921 ttcccgtttc ggagaaggtg gtctccagcg cctgaagggg ggaccttccc ctccggattt
     1981 ccccgtggaa gcgatgatag accatgatca tgtaatcgac gtacaacccg atcagcatcg
     2041 cggcgacgat gctcgtgacg atgttcagac ccccgtacag caggtaagcg aacaggagcg
     2101 tccaggagag ggcggcgaac agcgtcagga cgaaagcgca aaggacggcc acggggggac
     2161 gatacgccgc gtaaacgagc accaggacca ggacgaagga ggagaggaag gaaaggacca
     2221 tgtcctgcca gatcacgccg ttcgcctccg cggcgctcgc gtactcccct gcaagaccga
     2281 ccttgatccc ggccccggac ccttcggagc ccttcgccgc aatcccggcc acctcccgct
     2341 a
//
LOCUS       DASVJL010000143        14102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_302886, whole genome shotgun sequence.
ACCESSION   DASVJL010000143 DASVJL010000000
VERSION     DASVJL010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 14102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14102
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302886"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VF357_02810"
     CDS             <1..611
                     /locus_tag="VF357_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7523105.1"
                     /translation="LPFAEEIGFITAIDEWVLKTAVARVKEWHNAGLPHLRLSVNLSA
                     KQLQKSDLVERIAGILRETGFDPHHLDVEIAESVAMRNLEQTIPKISKLAGMGVGISI
                     DNFGTGYSSTNYLEKLPIQKLKIDKSVIKDIATDSYRRAIISAAIDMARKMKLGVIAV
                     GVETEEQLEFLRSIGCQEMQGFLFSRPLPEEEIRDLIATRER"
     gene            complement(666..3410)
                     /locus_tag="VF357_02815"
     CDS             complement(666..3410)
                     /locus_tag="VF357_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7523106.1"
                     /translation="MVLPAFQCISAIFLFVAAWQSVVFLSTGRLGRVWSFVSLALFLK
                     GLLSVWVIFSSSWFTSFTDLPVVIAEFFISLLLASGFLLTGQWFRFRERIEARFELIA
                     EVERSLVGVLEEKKVLSLVCGILSRARGYRLAWVGAAEADGTVRVECSAGDAVGFLRE
                     VALRWDDTPAGRAPPGTALRTGGTITAREIRGGLPAEWRDGCRRRGLVRCAAARIEQN
                     GFQHKVLVVHAGTSSAFDAVETEALAAMARRVGSAIQGARRHEIFVNAKASYDELLRN
                     QRDGVILVREGKVVRANPAAAEMLGYASPGLLFDADPVSILADPGAVVGLRDELLEPG
                     KGEPRSEWEASLLRVDGSTFPGEIRLTWAPREGRNDSFVPKRRGPLGMIVLRDATERV
                     RVLRELRKERDFSNRMLEISGALILQVSRSGEILLFNRQCEDVTGIAARDALGKKMWD
                     LLTEEPARDLHRDAIRAIVAGRMPPPLETSIVTAHASPRTIAWNHAPLHDGAGMIVSV
                     IVTGIDVTERRLLEKQLIEMQKMEAVGTLAGGIAHDFNNILTGILGSLDLARGFVPPG
                     SPASAPIAEGIKASERAVQLVRQLLDFSRRAPAECRPVNLGNVAREVTALFSQTIDRR
                     IEVTCTIADDLLPAFVDPNQVHQVLMNLCVNARDAIMERLETVEKSGGRPLTGYWIYT
                     RAENVEVDEDYCRIFPYARKGRFIRLSIGDNGSGMDEATQRRVFEPFFTTKKMGRGTG
                     LGLSTVYGIIKQHNGWINLESRTGKGTTFCAYFPEATGLPEERQELPETMPSRTGKEP
                     VLFADDEELIRDLGRQVMEMHGYTVFLAEDGMRAIDLFKSNREAIDLVVLDLTMPQRS
                     GMEVLRAIREIDPGIRVILSSGSPPAEPAPGTTFLPKPYRAEMLAKVVRSALDTPRTA
                     "
     gene            3543..5024
                     /locus_tag="VF357_02820"
     CDS             3543..5024
                     /locus_tag="VF357_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX7523107.1"
                     /translation="MVNESGKLYTVDNIVPQEMRMTNGARSDSIVRGFALSSVFWLVV
                     GLVVGLWLAAEMVFPALNLAPWLAFGRLRVVHTNGLIFGFTIAGIFACSFYMLEKLTR
                     TPLAFPGLAKAQLWLFNIAIALAALSLFAGMNTSKEYAELEWPLDIVVVILWVMFAVN
                     VMGTLVKRREKQMYVSLWFLIACVITVAVVYILNNLATPVSLTKSYSAYAGVNDANVQ
                     WWFGHNAVASVFTFPILAMFYYFLPKSTGLPIYSHRLSIIAFWSLVFGYLWTGAHHLM
                     LTPVPEWIQTVALAFSLFLIAPSWASVINGYNTMNGNWEKMKSNYLVKFFILGITFYG
                     LQTIQGPTQAIRALTSHLHYTEWVVGHVHMGTMGWVTMIITASMYFMMERISGREIHS
                     VKLANVHFWLILVGQLLFTVTLWIAGIVQGAMWKATNPDGSLMYTFLDSVAAMYPYWT
                     VRLVGGLLYFAGITVFAYNLYMTSRTKPKAKSPAAAAAKVQAA"
     gene            5040..6026
                     /gene="ccoO"
                     /locus_tag="VF357_02825"
     CDS             5040..6026
                     /gene="ccoO"
                     /locus_tag="VF357_02825"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF011055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEX7523108.1"
                     /translation="MRFDSLYEKPVLFAIAVVVVISIGTLVTTFIPLFLPSTQPVSPL
                     VKPYTAVETEGRDIYIREGCNNCHTQTVRPLRSEVARYGDYSKPEESAWDRPFLWGSR
                     RTGPDLARVGGKYPDAWHYRHMANPQAMFDKSNMPAYPWLAKAKLDTSLTARKVKILG
                     YGYDDAEVARQLAVFRKTVTAPAYPSAQVRAQVTPVALQGKVTELDALVAYLQKLGRD
                     LKAAQKSVPGAAVAEAAESRNPYAGNHAAEEEGERIFKENCRSCHGEKGAGGFGPKLA
                     TTAHKYGGSDAELFASIAGGRPGGMPTFLPQLGKDRVWKAVAYIRHLERESR"
     gene            6023..6202
                     /locus_tag="VF357_02830"
     CDS             6023..6202
                     /locus_tag="VF357_02830"
                     /inference="COORDINATES: protein motif:HMM:NF017366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEX7523109.1"
                     /translation="MTPRALAYLFFTLLLAGIFAGIIAYYFNRKRYDRVEAPKHRMLA
                     DDDPLPDSTGSRREG"
     gene            6207..6689
                     /locus_tag="VF357_02835"
     CDS             6207..6689
                     /locus_tag="VF357_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7523110.1"
                     /translation="MEEPIGKLEAHNKVPRGFLVLLFGLIAFGVYYIAAYTPGISGWS
                     QYKVLSKEMEAEKANAAAGAAKMNENHYERDEKAVAEGQVIYTGKCAECHGKDLKGAD
                     GSSLIGHLKYGEQEPGMYESIAKGRPGGMPAFETELGRDRIWKVLAYVDSVREHGKKP
                     "
     gene            6955..8013
                     /locus_tag="VF357_02840"
     CDS             6955..8013
                     /locus_tag="VF357_02840"
                     /inference="COORDINATES: protein motif:HMM:NF025121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7523111.1"
                     /translation="MALLLPFLRIRGRSAVRFDLPTLSLHIFGAVVPIDEFYLVLLGV
                     LFLVALTLWVTVVFGRLWCGWLCPQTAIGEIGEWIASALPLRFRSGGKTAMLLPFSAL
                     VSLSLLWYFGPPAEATRGLFRSPILLGFFLAQWAGIYIMVAVMGPRFCKTACPYAMLQ
                     SVLADRETLAVAYDPARAECLRCDRCIRVCPVGIDLRKGGQRECVACAACIDACREVT
                     SRRNLAPFIAYRGTVLRGKAYLFAGGCLAGALVLLAAIWSRPDVRFAVQWEGKAGTPM
                     GNVYRYSVRNDSDRPVALALSVERPARLLDDPEVAVPSRGRVTGTVTVQGGNGTPGEI
                     LITAAGRGFRIVRKAAFP"
     gene            8010..8774
                     /locus_tag="VF357_02845"
     CDS             8010..8774
                     /locus_tag="VF357_02845"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX7523112.1"
                     /translation="MKRLIGGLFAAFALGTGATIYIAHRSWDGLVDRRYSEAGSNEFA
                     EREAEARSGFTATLPDRYRAGENRFRALLSTSAGPLRGARVTLDAMRIEGPRDDRSVT
                     LREEAPGAYAADVFLPLPGQWTFSLSVDADDLHARRRWTAVAVPSDAAGVLRGAAGGQ
                     EVLLSITPWPPIAMREADFTVSLPGYAGKASPYVDLSMAGMEMGRNRVDLSPGPDGRY
                     RGTGVIVRCPSGKHDWEATVTAPGAGKAVFHFAVAD"
     gene            8758..9453
                     /locus_tag="VF357_02850"
     CDS             8758..9453
                     /locus_tag="VF357_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7523113.1"
                     /translation="MPWPTDSLPWILFVTGLVGGAGHCVGMCGPLVAGYAITLRNHAA
                     TLPHLLFHVGRVTTYGIAGGIVGASGSFVRVTAWVAPFQSFLLAGTGVLIALMGLSVG
                     GWLPWARRIEGSGPFHGVLVGVARRAAEAGGPGAAFPLGMATGLLPCGLVYTALLSAA
                     RSGMEGGSPADGFVRGFLAMAAFGAGTFPALFLFGKVVAAAGPRLRGALAKAAAALLV
                     AAGVMFAARAFLR"
     gene            9468..11873
                     /locus_tag="VF357_02855"
     CDS             9468..11873
                     /locus_tag="VF357_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX7523114.1"
                     /translation="MAVCAHCLLEVPEESATRETIDGKETIFCCPGCRAIHSLLRSEG
                     LTGFYARRHGWTPGPPESARVPLDAFDGAVRTSGRQAEADLVISGIRCASCVWLIERY
                     LGGRPGILSTRVNFATGRAGISWNPSETGIGDVVLAIRSLGYTPYPPESLPGGDALRR
                     ETSDLLLRFGTAAFLSMQVMLFTAGLYAGYFQGIDARYAQLFRWLCFLLATPVVFYSG
                     APFFRGALRGARHGAFGMDALVSLGAFSAYGYSTASLFLGGEVYFDTATMILTLILLG
                     RYIEAGARSRAADGISRLVRLAPVMARKAMPGGGTIDAPVASLSPGDLVEIVPGERMP
                     VDGNVIDGNSEADEAMLTGESAPVSKIAGDPVVAGSLNGTGRLLVRVVRTGGETVLSR
                     VVQAVEEAQARKAPIQRTADRMVRFFVPAILLIAAGTVFFGLHGGSLPHAALMAGISV
                     LVIACPCALGLATPLAVLVGTTSAQARGILVRGGDVLEQAARVRCVLFDKTGTLTLGR
                     PRLTDVEGIGIGREDALRLAASLEASSEHAIGRAIADALPASRRLPVHRFRAHPGEGI
                     EGEIEGVRYLLGRPELLERFGIAVDGDASRAYAALSAMRRTAILLSDGSRPLAIFATE
                     DALRPEAAGSVSLLRASGTAVAMVTGDDPGVAARVAGEVGIRDIRARVTPAGKREEVR
                     RARERFGPVLVVGDGVNDALALAEADVGVAMGRGTGLAIHSAGATLMTEDLLRIPAFL
                     ALSRATMRVIRQNLFWAFSYNLVAIPLAVAGKLHPIAAAAFMVGSSLLVVGNSLRLRR
                     GGR"
     gene            11876..12082
                     /gene="ccoS"
                     /locus_tag="VF357_02860"
     CDS             11876..12082
                     /gene="ccoS"
                     /locus_tag="VF357_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEX7523115.1"
                     /translation="MWTTLLLIAVSLALGFAAWLLFLWAVKSHQYDDVERPKHRMLDD
                     DDEVRDTDGESRRRKERTDENRRG"
     gene            12122..12544
                     /locus_tag="VF357_02865"
     CDS             12122..12544
                     /locus_tag="VF357_02865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7523116.1"
                     /translation="MSELPVTYRGVTYPWQCDHMNHMNVMWYVGKFDEATWNLFSMIG
                     LTPSYFREKQCGLAAVQQNITYKRELHPGDVIVIRSEILEVKEKMIRFLHEMRKEETQ
                     EIAATSELISVHIDTSLRKSCPLPKEVIERARGMIAGG"
     gene            complement(12507..12875)
                     /locus_tag="VF357_02870"
     CDS             complement(12507..12875)
                     /locus_tag="VF357_02870"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7523117.1"
                     /translation="MKLLEVKEGYARLSVRVEERFLNAHNIGHGVLVFAAADAANALS
                     VNSVRDEVCAHSSLHILRAAKPGEEVIGESLVLHSDSQATVCEITVTSGEGRLLAKGQ
                     WTTMPRGNVTLPRSFPGRVR"
     gene            complement(12960..>14102)
                     /locus_tag="VF357_02875"
     CDS             complement(12960..>14102)
                     /locus_tag="VF357_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7523118.1"
                     /translation="ATMIQAGLPVLSFGIFAKLLPEFQFEERVGNSLVVREPVGVVAA
                     ITPWNYPLHQIAAKVAPALAAGCTIVLKPSEVAPINAFLLAEIIDAAGLPKGVFNLVT
                     GYGAVVGEALAGHREVDMISFTGSTRAGKRVSELAARSVKRVALELGGKSASILLEDA
                     DLAAAIKGTVSACFLNSGQTCAAHTRMLVPESKYAEAKQLVVAEASKYTVGDPFSETS
                     KLGPLVSSAQRERVRGYIKKGIEEGAELLCGGPEAPDALPTGWYVVPTVFGRVHPDST
                     IAQEEIFGPVLSIIVYKDEEEAVAIANNSMYGLGGGVWSKDEAHAVRVARRIRTGQVD
                     INGGPFNLQAPFGGHKQSGHGREYGRAGLEEFLEYKSLQFKPERKT"
BASE COUNT         2339 a         4626 c         4642 g         2495 t
ORIGIN      
        1 ttcttccttt tgccgaagag ataggattca ttaccgcgat tgacgaatgg gtcctcaaga
       61 ccgcggtcgc ccgggtaaaa gaatggcata acgcaggact cccccatctt cgtctatcgg
      121 ttaacctttc ggcgaaacaa ctccagaaat cagacctcgt ggagcggatc gcagggatac
      181 tgcgtgagac cggattcgat ccgcaccatc tcgatgttga aattgccgag agcgtagcga
      241 tgagaaatct ggaacagacg atccccaaga tcagcaagtt ggccgggatg ggtgtaggca
      301 tttcgatcga caacttcggt accggctatt catcaaccaa ttacctggag aaactgccca
      361 ttcaaaagct taagatcgat aaatccgtca tcaaggatat cgctaccgac tcttaccgca
      421 gagcgattat cagcgcggca atcgatatgg cccggaagat gaaactcggg gttattgccg
      481 tgggagttga gacggaggaa cagctcgaat ttctcaggtc tataggatgt caggagatgc
      541 agggattcct cttcagcagg ccgttacctg aggaagagat ccgggatttg attgcgacca
      601 gggagagatg aggatggaag gaggctggcc gaagcaggat gttcaagttg cttcggccag
      661 cctccttacg ccgtacgagg ggtgtcgagg gcggagcgaa cgaccttcgc aagcatctcg
      721 gcgcgatacg gcttcggaag gaatgtcgtc ccgggagccg gctcggcggg ggggctgccg
      781 ctggagagga tgacccttat gccggggtcg atttcccgga tcgcgcgaag gacctccatc
      841 ccggaccgtt gcggcatcgt gaggtcgagc accacgaggt cgatcgcctc ccggttcgac
      901 ttgaaaagat cgatcgcccg catgccgtcc tcggccagga acaccgtgta cccgtgcatt
      961 tccatcacct ggcggccaag gtcgcggatc agctcctcgt cgtccgcgaa cagcacgggc
     1021 tccttccccg tcctggaagg catggtctcc gggagctcct gcctctcctc cggaaggccc
     1081 gtcgcctcgg ggaagtaggc gcagaaggtc gtcccctttc ccgtgcggct ctccaggttg
     1141 atccacccgt tgtgctgctt gatgatgccg tacacggtgg acagccccag ccccgttccc
     1201 cgccccatct tcttcgtggt gaagaacggc tcgaacaccc gccgctgcgt cgcctcatcc
     1261 atcccggagc cgttgtcgcc gatggagagt cggataaagc ggcctttccg ggcgtacggg
     1321 aagatccggc agtagtcttc gtccacctct acgttctccg cccgggtgta gatccagtac
     1381 cccgtcagcg gccgtccccc cgacttctcg acggtttcga gtctctccat aatggcgtca
     1441 cgggcgttga cgcacaggtt catgaggacc tggtggacct ggttggggtc gacaaaggca
     1501 gggagcaggt cgtccgcgat cgtgcaggtc acctcgatcc ggcggtcgat cgtctgggaa
     1561 aagagggcgg tcacctcccg ggccacgttc ccgagattca cggggcggca ctccgcgggg
     1621 gcgcgccggg agaaatcgag gagctggcga acgagctgca ccgcgcgttc cgaggccttg
     1681 atcccttctg cgatcggggc cgacgccgga gagccgggcg gcacgaaccc gcgggcgagg
     1741 tcgagactgc cgaggatccc cgtaaggata ttgttgaaat cgtgcgcgat ccctccggca
     1801 agtgtcccga cggcttccat tttctgcatc tcgatcagct gcttctcgag caggcgccgt
     1861 tccgtgacgt cgatcccggt cacgatgacg gagacgatca tgccggctcc gtcgtgcagc
     1921 ggggcatggt tccaggcgat ggttcgcggc gaagcgtggg cggtgacgat cgaggtttcg
     1981 agagggggcg gcatgcgccc cgcgacgatc gcccggatgg cgtcgcggtg gaggtcgcgg
     2041 gccggctcct ccgtcagcag gtcccacatc ttctttccga gcgcgtcccg ggcggcgatc
     2101 cccgtcacat cctcgcactg ccggttgaag aggaggatct ccccggacct gctcacctgc
     2161 agtatgagag ccccggagat ctccagcatc cggttcgaga agtcgcgctc cttccggagt
     2221 tcccggagga cccgcaccct ctcggtcgcg tcgcgaagga cgatcatccc gaggggaccg
     2281 cgtcgcttcg ggacgaaaga gtcgttccga ccttcccggg gcgcccaggt gagacggatc
     2341 tcccccggga aggtcgagcc gtccacgcga aggagagagg cctcccactc gctccggggc
     2401 tctcccttgc cgggctccag caattcgtcc cgcaggccga ccaccgcgcc gggatccgcc
     2461 aggatcgaaa ccgggtcggc gtcgaacagc agccccgggg acgcgtaccc gagcatctcg
     2521 gcggcggccg gattggcgcg caccaccttc ccctcgcgca cgagaatgac cccgtcccgc
     2581 tggttccgga gcagttcgtc ataggacgct ttcgcgttaa cgaagatctc atggcgcctg
     2641 gccccctgga tcgcgcttcc cacccggcgg gccatcgcgg cgagcgcctc cgtctcgaca
     2701 gcgtcgaagg cggaggacgt cccggcgtgc acgacgagaa ccttgtgctg gaagccgttt
     2761 tgctcgatcc gggccgcggc gcagcgaacg agaccgcgac gccggcaacc gtcccgccac
     2821 tccgcgggaa gcccgccgcg gatttccctc gcggtgatcg taccgccggt ccgcaaggcg
     2881 gttccgggcg gcgcccgccc tgcgggggtg tcgtcccagc gaagagcgac ctcccggaga
     2941 aaccccacgg cgtcccccgc gctgcactcc acgcggaccg ttccgtcggc ttcggcggct
     3001 cccacccacg cgaggcggta cccgcgggca cgggagagga tgccgcacac cagggagaga
     3061 actttcttct cctccaggac acccaccagg gaacgttcca cctcggcgat cagctcgaac
     3121 cgcgcctcga tgcgctccct gaagcggaac cattggccgg tgaggaggaa gcccgaagcg
     3181 aggagcaggg agatgaagaa ttcggcgatc acgaccggta agtcggtgaa cgaggtgaac
     3241 caggaagagg agaagatcac ccagaccgag agaagcccct tcaggaagag ggcaagggag
     3301 acgaaggacc agacacggcc gagccggccg gtcgaaagaa acacgacgga ctgccacgcg
     3361 gcgacgaaca ggaagatggc cgagatgcac tggaacgcag gaagcaccat ccggttatac
     3421 cactcccctg atttctttgg agaacaactc ccgttatccc tatcggcggg atccgcccgg
     3481 aacttcaggg aatgttccga tcggacggaa ttctaccgga atcggagagc cgccccaccc
     3541 ttatggtgaa cgaatccgga aaattgtaca ctgtcgacaa catcgtccca caggagatgc
     3601 gaatgacgaa cggggcacga agcgactcca tcgtgagggg attcgccctc tcctcggtgt
     3661 tctggctggt ggtcgggctg gtggtcggac tgtggctggc ggccgagatg gtcttcccgg
     3721 cactcaacct tgcgccgtgg ctcgcgttcg ggcgcctgcg cgtggtccac accaacgggc
     3781 tgatcttcgg cttcaccatc gccgggatct tcgcctgcag cttctacatg ctggaaaagc
     3841 tgaccaggac cccgctcgcc tttccgggcc tcgcgaaggc ccagctctgg ctgttcaaca
     3901 tcgccatcgc cttggcggcc ctctccctgt tcgccgggat gaacacctcc aaggagtacg
     3961 cggagctgga gtggcccctc gacatcgtgg tggtgatcct gtgggtgatg ttcgccgtaa
     4021 acgtgatggg aacgctcgtc aagcggcggg agaagcagat gtacgtctcc ctctggttcc
     4081 tgatcgcctg tgtcatcacg gtggcggtcg tctacatcct caacaatctc gcgaccccgg
     4141 tgagcctgac caaatcgtac tcggcgtacg ccggcgtgaa cgacgcgaac gtccagtggt
     4201 ggttcggaca caacgccgtc gcctccgtct tcaccttccc gatcctcgcg atgttctact
     4261 atttcctgcc gaaatccacg gggctgccga tctacagcca ccggctctcc atcatcgcct
     4321 tctggtccct cgtcttcggc tacctctgga cgggggcgca ccacctgatg ctgaccccgg
     4381 tcccggaatg gatccagacc gtggcgctgg cgttcagcct gttcctcatc gccccctcct
     4441 gggcttcggt catcaacggc tacaacacga tgaacgggaa ctgggagaag atgaagtcga
     4501 actacctggt caagttcttc atcctcggca tcacctttta cgggctgcag acgatccagg
     4561 gaccgaccca ggcgatccgg gcgctcacct cccacctcca ttacacggag tgggtggtcg
     4621 gccatgtcca catgggaacg atgggttggg tgacgatgat catcacggcg tcgatgtatt
     4681 tcatgatgga gaggatctcc ggccgggaga tccacagcgt gaagcttgcg aacgtccatt
     4741 tctggctgat cctcgtgggg caactcctgt tcaccgtgac cttgtggatc gcgggcatcg
     4801 tccagggcgc catgtggaag gccaccaacc ccgacggctc cctcatgtac acgttcctcg
     4861 actccgtggc cgcgatgtac ccgtactgga ccgtgcggct cgtcggcggc cttctctact
     4921 tcgccgggat cacggtcttc gcctacaacc tgtacatgac ctcccggacg aagcccaagg
     4981 cgaaatcgcc agccgcggct gcggcgaagg tccaggcggc ctgagcaagg agacgacgga
     5041 tgcgcttcga cagcctctac gaaaaacccg tgttgttcgc catcgcggtg gtggtcgtca
     5101 tctcgatcgg gacgctggtg acgaccttca tccccctctt ccttccgtcg acccagcccg
     5161 tgagccccct ggtcaagccg tacacggccg tcgagacgga agggagggac atctacatcc
     5221 gggaagggtg caacaattgc cacacccaga cggtccgtcc gctgcgttcc gaggtcgccc
     5281 ggtacggcga ctactccaag cccgaggagt cggcctggga ccggccgttc ctctggggtt
     5341 cccgtcgcac ggggccggac ctggcgcgcg tcggcggcaa atacccggac gcgtggcatt
     5401 accgccacat ggcgaacccg caggcgatgt tcgacaagtc gaacatgccg gcgtacccgt
     5461 ggctcgcgaa agcgaagctc gacacctccc tcacggccag gaaggtgaag atcctcggat
     5521 acgggtacga cgacgcggaa gtggcccggc aactcgcggt gttccggaaa accgtgacgg
     5581 ctccggcata cccttccgca caggtccgcg cccaggtgac cccggtcgcc cttcagggga
     5641 aggtcacgga actcgacgcg ctggtggcgt atctccagaa gcttgggcga gacctgaaag
     5701 cggcgcagaa atccgtcccg ggagccgccg tcgccgaggc ggcggagtcg aggaatccgt
     5761 acgccggaaa ccacgcggcg gaggaggaag gcgagaggat cttcaaggag aattgccgaa
     5821 gctgccacgg tgaaaagggt gcgggggggt tcggcccgaa gctggcgacg accgcccaca
     5881 agtacggcgg ttcggacgcg gagctgttcg cctcaatcgc gggagggcgc ccgggaggga
     5941 tgcccacgtt ccttccgcag ctcgggaagg accgggtgtg gaaggcggtc gcctacatac
     6001 gccaccttga aagggaaagc cgatgacgcc gcgggcgctg gcgtacctct tcttcacgct
     6061 gctcctcgcc gggatcttcg ccggaatcat cgcttactat ttcaatcgga agcggtacga
     6121 ccgcgtggaa gccccgaagc accggatgct ggccgacgac gatccgctgc ccgattccac
     6181 gggcagccgc cgggagggat gaaccgatgg aagagccgat cgggaagttg gaggcgcaca
     6241 acaaggtccc tcgcgggttc ctcgtccttc tgttcgggct gatcgccttc ggcgtctact
     6301 acatcgccgc gtacactccg gggatcagcg gctggtcgca gtacaaggtg ctttcgaagg
     6361 agatggaggc ggaaaaggcg aatgccgccg ccggcgccgc gaagatgaac gagaaccact
     6421 acgaacggga tgaaaaggcc gtggcggaag ggcaggtcat ctacaccggg aaatgcgccg
     6481 aatgccacgg aaaagacctg aagggagcgg acggctcttc cctgatcggc cacctgaagt
     6541 acggtgaaca ggagccgggg atgtacgagt cgatcgccaa gggccgcccg ggagggatgc
     6601 ccgcgttcga gacggaactg ggacgggacc ggatctggaa ggtgctggcc tacgtcgact
     6661 cggtccggga gcacgggaag aaaccttaac ccgcatttct caccaggctt ctactgcggc
     6721 ggcggatatg ggcatgtcta cagcgttgca ctcggtcggg ctcctcgacg tagtgtcaac
     6781 tacgcctccg gggccctcgg tcgcgcgcct tgtatccacg cccatctcca ccacctcgct
     6841 acgaaccctg gtgagaaaag cgggttaacg gatcctcccc ccttcccgcc cccgggttac
     6901 actcgttcca tgtggaaaaa ccgccgacgg atcgtcgccc tccttggtgg ggcggtggct
     6961 cttctccttc cgttcctccg gatccgcgga cgcagcgcgg tccggttcga ccttcccacc
     7021 ctctcgctcc acatcttcgg tgcggtcgtc ccgatcgacg agttctatct ggtcctgttg
     7081 ggtgtgctct tcctcgtggc gctcaccctt tgggtgacgg tcgtcttcgg gcgattgtgg
     7141 tgcgggtggc tttgtccgca gacggcgatc ggcgagattg gagagtggat cgcctccgcg
     7201 ctcccgctcc gtttccggtc cggcggaaag acggcgatgc tgctcccctt ctccgccctg
     7261 gtgtccctct cccttctgtg gtacttcggc ccgcccgccg aggcgacccg cggcctcttc
     7321 cggtccccga tcctcctcgg cttcttcctc gcgcagtggg cagggatcta catcatggtg
     7381 gccgtcatgg gcccccgttt ctgcaagacg gcgtgcccgt acgcgatgct ccagagtgtc
     7441 ctcgccgatc gagagacgct cgcggtcgcg tacgacccgg cgcgggccga atgcctccgg
     7501 tgcgaccggt gcatccgggt ctgccccgtg gggatcgacc tccggaaggg cggacagcgg
     7561 gagtgcgtcg cttgcgcggc gtgcatcgac gcctgccggg aggtgacgtc gcgccggaac
     7621 ctggcgccct tcatcgccta ccggggaacg gtcctgcgcg ggaaagcgta cctgttcgcg
     7681 gggggttgcc tcgcgggcgc gctcgtcctc ctcgccgcga tctggagccg tcccgacgtc
     7741 cggttcgccg tgcagtggga agggaaggcc gggacgccga tggggaacgt ctaccggtac
     7801 tccgtgcgga acgactcgga ccggccggtc gccctcgcgc tgtccgtcga gcggccggcg
     7861 cgactcctcg acgatcctga agtcgcggtg ccttcccggg ggcgcgtgac aggcacggtg
     7921 acggtgcagg gggggaacgg gacgccggga gagatcctga tcaccgccgc ggggcggggg
     7981 ttccgcatcg tccggaaggc ggcgttcccg tgaagcggct gatcgggggg ctgttcgccg
     8041 ccttcgcgct cgggacgggg gcgacgatct acatcgccca tcgttcctgg gacgggctcg
     8101 tcgatcggcg gtattccgaa gcggggtcga acgagttcgc cgaacgggaa gcggaggcgc
     8161 gatcggggtt cacggcaacg ctccccgacc ggtaccgcgc cggggagaac cggttccgcg
     8221 ccctcctgtc cacctcggcg gggccgcttc gcggcgcccg cgtcaccctc gacgcgatgc
     8281 ggatcgaggg accccgcgac gatcgctcgg tcacgctccg ggaggaagcg cccggcgcct
     8341 acgccgcaga cgtcttcctc cccctccccg gacagtggac cttttccctc tccgtggacg
     8401 cggatgacct ccacgcccgg cgacgctgga cggccgtcgc agtgccgtcg gacgcggcgg
     8461 gcgtcctgcg gggcgccgcc ggcggacagg aggtcctcct ctcgatcacc ccatggccgc
     8521 caatcgcgat gcgggaggcc gatttcaccg tatcccttcc aggatatgca gggaaagcct
     8581 cgccctacgt cgatctgtcg atggcgggga tggagatggg acggaaccgc gtcgatctct
     8641 cccccgggcc cgacggtcga tatcgcggca cgggagtgat cgtccgatgc ccaagcggga
     8701 agcacgactg ggaagcgacg gtgactgcgc cgggcgccgg gaaggcggtt ttccattttg
     8761 ccgtggccga ctgactccct cccatggatc cttttcgtca ccggactggt gggcggggcc
     8821 ggccattgcg tcgggatgtg cggtccgctg gtcgccgggt acgccatcac gttgcggaac
     8881 catgcagcca cccttccgca ccttctcttc cacgtgggcc gggtgaccac gtacggcatc
     8941 gcggggggaa tcgtcggggc gtcgggctcc ttcgtccgcg tcacggcgtg ggttgccccc
     9001 ttccagtcat tcctcctcgc cgggaccggc gtgctgatcg cgctgatggg gctttcggtc
     9061 ggcggttggc tgccgtgggc gcgacggatc gaggggagcg gccctttcca tggcgtcctc
     9121 gtcggggtcg cgcgacgcgc ggccgaggcg ggcggcccgg gcgccgcctt ccccctcgga
     9181 atggcgacgg gactgctccc gtgcggactg gtctacaccg cgctcctctc ggcggcgcgt
     9241 tccgggatgg agggaggctc cccggccgac gggttcgtcc ggggattcct cgcgatggcc
     9301 gccttcgggg cgggaacgtt tcccgccctg tttcttttcg gcaaagtggt cgcggcggcg
     9361 ggaccgcgtc tgcgcggggc gctggcgaag gccgccgccg cccttctcgt cgctgccggg
     9421 gtgatgttcg ccgcgcgcgc gtttctccgt tgagcgggaa tcctgcaatg gcggtctgcg
     9481 cacactgtct cctcgaggtg ccggaggagt ccgcgacccg ggagacgatc gacgggaagg
     9541 agacgatctt ctgctgcccc gggtgccggg cgatccacag tctcctgcgc tccgaagggt
     9601 tgaccggctt ctacgcccga cgccacgggt ggactcccgg ccctccggag agcgcccggg
     9661 ttccgctcga tgcgttcgac ggggccgtcc gcacctcggg ccgacaggcc gaagcagatc
     9721 tcgtcatctc cgggatccgt tgcgcctcct gcgtctggct catcgagcgg tatctcgggg
     9781 ggcgacccgg cattctttcc acgcgtgtca atttcgccac gggcagggcg ggaatctcct
     9841 ggaatccgtc ggaaaccggg atcggagacg tcgtcctggc gatccgttcc ctcgggtaca
     9901 cgccgtaccc ccccgaatcg ctccccggcg gagacgccct tcgacgggaa acgtcggatc
     9961 tgctgctgcg cttcgggacc gcggcgttcc tatcgatgca ggtgatgctc ttcaccgcgg
    10021 gactctacgc cgggtacttc caggggatcg acgcgcggta cgcgcaactt ttccggtggc
    10081 tttgtttcct cctcgccact ccggtcgtct tctactccgg cgcgccgttc ttccgcggcg
    10141 ccctccgcgg agcgcgccac ggggcgttcg ggatggacgc cctcgtctcc ctcggcgcct
    10201 tctccgcgta cggctacagc accgcctcgc tgttcctcgg gggagaggtg tatttcgaca
    10261 cggcgacgat gatcctgacg ttgatcctcc ttggccggta catcgaggcg ggagcgcggt
    10321 cccgcgcggc cgacgggatc tccaggctcg tgcggctcgc gcccgtgatg gcccggaaag
    10381 cgatgccggg gggcggaacg atcgatgccc ccgtcgcctc cctttctccc ggggatctcg
    10441 tggaaatcgt ccccggggag cggatgccgg tggacgggaa cgtgatcgat ggaaattcgg
    10501 aggcggatga ggcgatgttg accggggagt cggcccccgt gtccaaaatc gcgggggatc
    10561 cggtcgtcgc cgggtcgctg aacgggacgg gacggctcct cgttcgggtc gtccggaccg
    10621 gcggagagac cgtcctgtcc cgcgtggtgc aggcggtgga ggaggcgcag gcgcgcaagg
    10681 cgccgatcca gcggacggcc gatcgcatgg tccgcttctt cgtgccggcg atccttctca
    10741 tcgccgcggg gacggttttc ttcggccttc acggaggatc cctcccccat gccgcgctga
    10801 tggcggggat ctcggtgctg gtgatcgcct gcccatgcgc tctcgggctg gccacgccgc
    10861 tcgccgtcct cgtggggacc acctccgcgc aggcgcgcgg gatcctggtc cgcggggggg
    10921 acgttctcga acaggcggcc agggtgcggt gcgtcctgtt cgacaagacg gggacgctca
    10981 ccctcggccg cccgcggctg accgacgtgg aggggatcgg gatcggacgc gaggacgccc
    11041 tgcggctcgc cgcatccctc gaggcgtcct cggagcacgc gatcggacgc gcgatcgcgg
    11101 atgcgcttcc cgcctcccgg cgcctcccgg tccacaggtt ccgggcgcac ccgggggaag
    11161 ggatcgaagg agagatcgaa ggcgtgcgct acctgctggg ccgcccggaa ctcctcgagc
    11221 gattcgggat cgccgtggac ggggacgcct cccgggcgta cgcggcgctt tcggcgatgc
    11281 gaaggaccgc gatcctcctt tccgacggtt cgaggcccct cgcgatcttc gccacggagg
    11341 atgccctccg gccggaggcg gccgggtccg tttccctcct gcgggcctcc gggaccgccg
    11401 tcgcgatggt cacgggagac gacccgggcg tcgccgcgcg cgtggccggg gaggtgggga
    11461 tccgagacat ccgcgcccgc gtgacaccgg ctgggaagcg cgaggaggtg cgccgcgcgc
    11521 gggaacggtt cgggcccgtc ctcgtcgtcg gcgacggcgt gaacgacgcc ctcgccctcg
    11581 cggaggcgga cgtcggggtc gcgatgggac gtggaacggg gctggcgatc cacagcgccg
    11641 gcgcgacgct gatgacggag gacctgctgc ggatccccgc cttcctcgcg ctgtcccgcg
    11701 ccacgatgcg ggtgatccgg cagaacctgt tctgggcctt ctcctacaac ctggtggcga
    11761 taccgctcgc ggtcgcgggg aagctccacc cgatcgccgc cgcggccttc atggtcggaa
    11821 gctccctcct cgtggtggga aactcgctgc ggctgcggag agggggccgg tagccgtgtg
    11881 gacgacgctt ctgctgatcg ccgtgtcgct ggcgctggga ttcgccgcct ggctgctctt
    11941 cctgtgggcg gtgaagagcc atcagtacga cgacgtggag cgtcccaagc accgcatgct
    12001 cgacgacgac gatgaggtga gggatactga tggggaatcc cgcaggagaa aggagcgaac
    12061 cgatgaaaac cgccgtgggt gacctctccg gccaatctta cttatcgtgg ggggtggaga
    12121 gatgtccgag cttccggtaa cctatcgcgg cgtaacctac ccttggcaat gtgaccatat
    12181 gaaccacatg aacgtcatgt ggtatgtcgg gaaattcgat gaagccacct ggaatctttt
    12241 ttccatgatc gggctcacac catcctattt tcgcgagaag cagtgcggct tggcggcagt
    12301 ccagcagaac atcacatata agcgggagtt gcacccgggg gatgtcatcg tcatccgttc
    12361 ggaaatcctg gaggtaaagg agaagatgat ccgctttctc catgagatga ggaaggagga
    12421 gactcaggag atcgccgcga cctcggaact gatcagcgtg catatagaca ccagcctgcg
    12481 aaaatcctgt ccgcttccga aagaggtcat cgaacgcgcc cggggaatga tcgcgggagg
    12541 gtgacgttcc cgcgcggcat tgtcgtccac tgtcccttgg ccaacagacg cccctccccc
    12601 gacgtgacgg ttatctcgca aaccgttgcc tggctgtcgg agtgaagaac gagggattct
    12661 ccgatcacct cttcacccgg ctttgccgcg cggagaatat gcaggctcga atgggcgcag
    12721 acttcgtcgc gcaccgaatt gaccgacaag gcgttcgcgg cgtcggccgc ggcaaacacc
    12781 agcacgccgt ggccgatatt atgggcgttc aaaaaccgct cctccaccct taccgacagc
    12841 cgcgcgtacc cttccttgac ctccaacaat ttcatcccga agtcccggat cagccgatct
    12901 ccattccata tatcctagat tcgccccaca ggcgatctcc ttcagtacgc tgacccggtt
    12961 caggtcttgc gctccggctt gaactggagg gatttgtatt caaggaactc ctccagcccc
    13021 gcgcggccgt attcccgccc gtggccggat tgcttgtgcc ctccgaaagg tgcctggagg
    13081 ttgaacggcc ccccgttgat atccacctgc ccggtgcgga tgcgccgggc cacccggact
    13141 gcatgggcct cgtccttcga ccacaccccg ccgcccaggc cgtacatcga gttgttggcg
    13201 atggccaccg cctcttcctc gtccttgtag acgatgatcg acagcacggg cccgaagatc
    13261 tcttcctgcg cgatcgtcga atccggatgg acacggccga acaccgtggg cacgacatac
    13321 cagcccgtgg ggagggcgtc cggcgcttcg ggaccgccgc agagcagttc ggccccttcc
    13381 tcgattccct ttttgatata gccgcggacc cgctcccgct gggcgctgga aacaagaggt
    13441 cccagtttgc tggtttccga gaatggatcg ccgacggtgt acttcgaggc ttcggcgacg
    13501 acaagttgct tcgcctcggc gtacttcgac tcgggcacga gcatgcgggt atgggcggcg
    13561 caggtctgcc ccgagttgag gaaacaggcg ctcaccgtcc ctttgatcgc agccgcgagg
    13621 tccgcatcct ccaggaggat cgaggcgctc ttgccgccca gctccagcgc aacacgcttg
    13681 accgatcggg ccgccagctc ggagacgcgt ttcccggcac gtgtggaacc ggtgaacgaa
    13741 atcatgtcca cctcgcggtg accggcgagc gcttccccca ccaccgcgcc atacccggtt
    13801 acgaggttga acaccccctt tggcagaccg gcggcatcga tgatctccgc caggaggaat
    13861 gcgttgatcg gcgccacctc ggacggtttc aatacgatcg tgcaaccggc ggccagcgcg
    13921 ggagcgacct tggcggcgat ctggtgcagc gggtagttcc acggcgtgat ggcggcaacc
    13981 acaccgaccg gctcgcgtac caccagggaa ttgccgaccc gctcttcgaa ctggaactcg
    14041 ggcagcaact tggcgaatat cccgaaactg agaaccggca ggccggcctg aatcatcgtg
    14101 gc
//
LOCUS       DASVJL010000144         3656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_678020, whole genome shotgun sequence.
ACCESSION   DASVJL010000144 DASVJL010000000
VERSION     DASVJL010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678020"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..675)
                     /gene="amrA"
                     /locus_tag="VF357_02880"
     CDS             complement(91..675)
                     /gene="amrA"
                     /locus_tag="VF357_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX7523119.1"
                     /translation="MTEAVGTGPEAYLDESQRKVLLGIARRALDGYLGAGKIPPEEEA
                     RGKLAAPGAAFVTLTKNGRLRGCIGYTEAVAPLFKVVQECAVAAATEDPRFPPVSSNE
                     LPSLRVEISVLTPMVPIRPEEVEVGRHGLMVVQGRKRGLLLPQVPEEWGWDRETFLDQ
                     TCVKAGLPPSAWREGATLRAFTAEVFGEEKKTAT"
     gene            complement(695..1810)
                     /locus_tag="VF357_02885"
     CDS             complement(695..1810)
                     /locus_tag="VF357_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7523120.1"
                     /translation="MGSVPHIPEIMEGASRIRVLVVDDEEFLRSIVRERLEIAGYSVE
                     EASNGSEALGMLESTGPYSVLLTDIRMPVMDGITLLGEWGKKSPGTAGIVMSANAELD
                     TAVHALKMGACDYITKPFNFDVLLITIENALRKKDLEQQLDDYRTNLEKKVKEQTDII
                     NLMYVRSIDAMIKALEAKDFYTRGHSQRVTLYSIAIAEELGVTGQELEDLHRASVLHD
                     LGKIGVREAVLNKAGKLSEEEFLEIVRHPETAVRILEPIPFFRPLLPAILHHHERFDG
                     KGYPSRLVGKNIPLASRIMTIADTFDAMTSTRAYRKALPVTDAIAEIRRCSGTQFDPD
                     IVPAFLACQPRIVIPGDVSLPEGFEETIPASFRPQPS"
     gene            complement(1879..2919)
                     /locus_tag="VF357_02890"
     CDS             complement(1879..2919)
                     /locus_tag="VF357_02890"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX7523121.1"
                     /translation="MDVGIFGNTGSGKSTLFRSLSGVASVSAQGKSAGVCAIKVPDER
                     VDRMAEIFRPKKVTYISIAFHDIDAGETDPLSPQALTELRSIEVLALVVRAFDDDYHP
                     APPGGLDPAAEFRALSSAIVLSDYLVAQKRIERMTKEAKRDAEWTVLHKAIAAFEREI
                     PLRNVDLSVEEERVLSGFRFVSRLPTLLILNVGEGEETAETYHDVTKEAEKAKVPLVR
                     LCAKVEEEIAQLSPAEQADFLKEMGIARSARDQLVRGAFEATRYISFLTVGEDEVRAW
                     NVREGSTAPTAAGKIHSDLEKGFIRAEVISSDDFLRCGSMAKAKTEGRLRLEGKEYVV
                     KDGDIVHVRFNV"
     gene            complement(2935..>3656)
                     /locus_tag="VF357_02895"
     CDS             complement(2935..>3656)
                     /locus_tag="VF357_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX7523122.1"
                     /translation="DDETFGTGGTIARYAATGAEVHLVTATRGEAGMVGDPPVTDREH
                     LGEVRAGELMAAAEILGITKVHFLGFLDGQLESVPREQLVERAVDLIRRVRPHVLVGF
                     GPDGISRHADHKVMCEVALSAFDRSADPTWYPGDGPPWAPVKLYQFEIAQEIFAGWDA
                     PLAGVPRARLTAFIDTSEQVETKIRAFHCHKTQAKDTRRLLERPGYREFARQETYVLA
                     RTRLSGLPLPEDDLLAGIPDE"
BASE COUNT          593 a         1246 c         1159 g          658 t
ORIGIN      
        1 gagttcgtcc ccgccctccc gaggaatgcc caggggaaag tgctcaaggc gcggcttcgc
       61 gatcgccaga cctcccgacg gccccgctga tcacgtcgcc gttttcttct cttcgccgaa
      121 cacctccgcg gtaaacgccc ggagcgtcgc cccttcccgc cacgccgacg gcggcaggcc
      181 ggccttcacg cacgtctggt cgaggaacgt ctcccgatcc cacccccact cctcggggac
      241 ctggggcagc aacagacccc gcttccgacc ctgcacgacc atcaacccgt gcctgccgac
      301 ctcgacctcc tcgggccgga tggggaccat gggggtgagg acggagatct cgacgcgcag
      361 ggaggggagt tcgttcgagg agacgggggg aaagcgcgga tcctcggtgg ccgcggccac
      421 cgcgcactcc tggaccacct tgaagagcgg cgccaccgcc tcggtgtagc cgatgcagcc
      481 gcgcagccgc ccgttcttcg tgagggtgac gaaagcggct ccgggggcgg ccagcttccc
      541 ccgcgcttct tcctcgggtg gaatcttccc cgcgccgagg tacccgtcca gcgccctgcg
      601 ggcgatcccg agcagcactt ttcgctgcga ctcgtccagg tacgcctcgg gtcccgtacc
      661 gacggcctcc gtcatgagat cacctccgcc gagttcagct gggctgcggc cggaacgaag
      721 cgggaatcgt ctcctcgaat ccctccggca gactcacgtc gccggggatg acgatcctcg
      781 gctggcaggc gaggaatgcg ggaacgatgt cggggtcgaa ctgggtcccg gagcagcgcc
      841 ggatctcggc gatcgcgtcg gtgacgggga gcgccttcct gtacgcacgg gtggaggtca
      901 tcgcgtcgaa agtgtcggcg atcgtcatga tgcgggatgc gagagggatg tttttcccca
      961 cgaggcgcga ggggtacccc ttcccgtcga accgttcgtg gtgatgcaga atggcgggga
     1021 gaagagggcg gaagaagggg atcggttcaa ggatccggac ggccgtctcg gggtggcgga
     1081 cgatctccag gaattcctct tccgagagct tcccggcctt gttcaggacc gcttcccgga
     1141 cgccgatctt cccgaggtcg tgcagcaccg acgcccggtg aaggtcttcc agctcctgac
     1201 ccgtcacccc cagttcttcc gcgatggcaa tggagtagag cgtcacacgc tgcgagtggc
     1261 ctcgggtgta gaagtctttc gcctcgagcg ccttgatcat cgcgtcgatg gaccggacgt
     1321 acatcaagtt gatgatgtcg gtctgctctt tcaccttttt ttcgaggttc gtacggtaat
     1381 catcgagctg ctgctcgaga tccttcttgc ggagcgcgtt ctcgatggtg atcagcagga
     1441 cgtcgaagtt gaagggtttc gtgatgtagt cgcacgcccc catcttgagc gcgtggacgg
     1501 cggtgtcgag ctcggcgttg gcggacatta cgatccccgc ggtcccgggg gacttctttc
     1561 cccactcgcc gagaagcgtg atcccgtcca tgacgggcat ccggatgtcg gtcagaagga
     1621 cgctgtacgg accggtgctc tcgagcatgc ccagcgcctc gctcccgttg gacgcctctt
     1681 caacggaata tcccgcgatt tcaaggcgtt cccggacaat ggaacggagg aattcctcgt
     1741 cgtcgaccac gaggacgcgg atccgggacg caccctccat aatttccggg atgtggggga
     1801 cagagcccat gttcccctta tcgccgcgga aaccggaaaa ctttacggaa gaatcgaaaa
     1861 aatcttccac cggcggattc acacgttgaa ccggacgtgg acgatatccc cgtccttgac
     1921 gacgtactcc tttccctcga gccgcagcct cccttccgtc ttggccttcg ccatcgaccc
     1981 gcaccgcagg aaatcgtcgg aggagatgac ctcggcccgg atgaatcctt tttccaggtc
     2041 ggagtggatt ttccccgcgg ccgtcggggc ggtgctcccc tcccgcacgt tccaggcgcg
     2101 tacctcgtcc tcccccactg taaggaagga gatataccgc gtcgcctcga acgccccgcg
     2161 aacgagttgg tcccgtgcgg accgggcgat ccccatctcc ttcaggaagt cggcctgctc
     2221 ggccggcgac agctgggcga tctcttcctc caccttcgcg cacaggcgca ccaggggaac
     2281 cttcgctttc tccgcctcct tcgtcacgtc gtggtacgtc tccgcggtct cctccccttc
     2341 accgacgttc aggatcaaaa gcgtcgggag gcgacttacg aaccggaagc ccgagaggac
     2401 ccgttcctcc tcgaccgaca ggtcgacgtt ccgaagggga atctcccgct cgaacgccgc
     2461 gatcgcctta tgcaggacgg tccactccgc gtcccgcttc gcctccttcg tcatccgctc
     2521 gatccgcttc tgggcgacga gataatcgga gaggacgatg gcggaggaga gtgcgcggaa
     2581 ttccgccgcc gggtccaggc cgccgggagg cgccgggtgg tagtcgtcgt cgaaggcccg
     2641 gacgaccagc gcaaggacct cgatgctccg aagctccgtg agggcctgcg gagagagggg
     2701 atccgtctcc cctgcgtcga tgtcgtggaa cgcgatcgag atgtaggtga ccttcttcgg
     2761 gcggaagatc tccgccatcc ggtcgacccg ctcgtccggg accttgatgg cgcacacccc
     2821 cgcgctcttc ccctgggcgc tcaccgaagc gacgccggaa agcgaacgga acagcgtgct
     2881 cttccccgat cccgtgtttc cgaagatccc gacgtccacc gtgccgcccc ttccttactc
     2941 gtccgggatc cctgccagca ggtcgtcctc gggcaagggt agcccggaaa gccgcgtcct
     3001 ggcaaggacg tacgtttcct ggcgggcgaa ctcccggtag ccgggacgct cgaggagccg
     3061 tcgggtgtcc ttcgcctggg tcttgtggca atggaaggcg cggatcttcg tctccacttg
     3121 ctccgaggtg tcgatgaagg cggtgagtct cgcccgcgga accccggcaa ggggagcgtc
     3181 ccacccggcg aagatttcct gcgcgatctc gaattgatac agcttgacgg gcgcccaggg
     3241 ggggccgtcg cccgggtacc acgtcggatc ggcggagcgg tcgaaggcgg acagggccac
     3301 ctcgcacatg accttgtggt ccgcgtggcg cgatattcca tcggggccga accccacaag
     3361 gacgtgggga cggacccgtc ggatcaggtc gacggcgcgc tccaccagct gctcccgcgg
     3421 cacgctctcc agctgcccgt cgaggaaccc gaggaagtgg accttggtga tcccgaggat
     3481 ctccgccgcc gccatcaact ccccggcgcg cacctcccca aggtgttcac ggtcggtcac
     3541 cgggggatcg ccgaccatcc cggcctcccc ccgggtcgcc gtcaccaggt gcacctccgc
     3601 ccctgtagcg gcatatcgcg cgatcgtccc gccggtgccg aacgtctcgt cgtcgg
//
LOCUS       DASVJL010000145         4270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_866811, whole genome shotgun sequence.
ACCESSION   DASVJL010000145 DASVJL010000000
VERSION     DASVJL010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866811"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1022)
                     /locus_tag="VF357_02900"
     CDS             complement(75..1022)
                     /locus_tag="VF357_02900"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7523123.1"
                     /translation="MTERLRRIDILLIAGAFLLLLPFLKFQRTTDYIIFCVFVLSFDL
                     IYGYMGRLSFGHMLFLGAGAYGATLFAEHVSPNPFLSILFAIVAAAAVGALLGPIVVR
                     TTGAAFALINLAFNQVGHFLVLIAFARYTGGEDGMSAVFSKVGFLDFQDKRVLYGFCL
                     ACLLLTCWGMKRLTTSPFGILLRMIKENETRVKFLGYDTFRYKWAAYTISAGVAGFSG
                     ALSIVNYGYVTPSFIDTNRNVEVIFASLIGGAGSIYGSLVGGVVFMAISNYLPTYIPR
                     WEMFLGFGLLLLVFRFRTGIWGALSALEVFRPRREGEGG"
     gene            complement(1019..1720)
                     /locus_tag="VF357_02905"
     CDS             complement(1019..1720)
                     /locus_tag="VF357_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523124.1"
                     /translation="MSVSLLTVDAVDAAYGSASVLHKVSLRVDPGEMVFIAGRNGAGK
                     TTLLKTIAGFMPPAAGTVRLEGRDLRGIRPENVARLGVRYVFQDKRVFSKLTVRENLE
                     LAAHPSGERMSEVVARMVSIYPAMEKFLDSKAGGLSGGQRQILLIGRALTGNPKILLV
                     DEPTEGLAAGVIDDIFRLLSEMKGRLSMLIVEQNLPVVARLADRVYVMKEGKIFQELA
                     TRAEIEATGALETNL"
     gene            complement(1717..2475)
                     /locus_tag="VF357_02910"
     CDS             complement(1717..2475)
                     /locus_tag="VF357_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523125.1"
                     /translation="MPEEIILETRGVTKRFDGFAAVSGVSYRLQEGESAGIIGPNGAG
                     KTTFFNLLTGMFPPTEGAVLYRGADITRIPAHGRVRRGIVRTFQLVSVFDSLTVRENL
                     VLALIRAGKEQTASARFFLEDAWSGNLSELSAGALGTVGLREKALWKTAELSYGDKRK
                     LEIALALCLRPSILLLDEPFAGLSDGEIVEVLELIHRVRKEFTLVIIEHKISRIVDLV
                     SRLSVMHEGKLIAEGGPTEVLADPLVRQVYWGRP"
     gene            complement(2537..3793)
                     /locus_tag="VF357_02915"
     CDS             complement(2537..3793)
                     /locus_tag="VF357_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7523126.1"
                     /translation="MRLAKRMAMAGIGLAAAWTLLLPAGAGAAAPDKIKVGFMFGMTG
                     AASVIGPVQLDGAKLAVKEINAAGGVKLGGKKVPVEFVVRDDESKPDIAIRRFRELLT
                     EEKVDLIVGQTFAPISGALNKEVKKTPVLYFPVNVVALKMFEKAEMAPSTFALHGSAY
                     AAGYAGAAYIVKNLGYKKIVFFGPAYAFGQDQWAGAKDALKKYGVTAEYLESPVGTSD
                     FTPYLTKVAEMKPDIVMLAHWGVDAINVLKQSYEIGLKKKTKIWFNWMTNVFGSGVPA
                     DALDGVYSLMSWYYDMTGFKDAAVVKAANDFSQKFRKEYGYPPDPYSAMAYLGTIEAM
                     RGIALAQSTDPAAVAAALMKSPRFDTMKGPATWRADHQPTFKYGAFVVVGKGAKERKD
                     PKWDLVKIVDVYSGEDYLPPLSALGY"
     gene            complement(3836..>4270)
                     /locus_tag="VF357_02920"
     CDS             complement(3836..>4270)
                     /locus_tag="VF357_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX7523127.1"
                     /translation="GFPELKKGGPGCDGLRGIEMDMKADWGTGAWLKGTPGPEIIKQL
                     TPKKGDIVVEGKMTLDAFHSTALDYLLRANQIEYVAFTGFHTNWCVESSARSAYDKGY
                     RVHVISDCTATDTAEEQNYAEKWIFPKIGKVMTSGEFLASLG"
BASE COUNT          747 a         1470 c         1306 g          747 t
ORIGIN      
        1 gaacgtcagc gacagcccga tggagaggat gtacaggatc cccccgatcg tgaacccgta
       61 gaccagcgtg gtaatcatcc gccctcccct tcccggcggg ggcggaacac ctcgagggcg
      121 gacaacgctc cccagatccc cgtccggaac cggaacacga gaaggagcag cccgaagccg
      181 aggaacatct cccacctggg aatgtacgtg gggaggtagt tgctgatcgc catgaaaacc
      241 acccccccca ccagggagcc gtagatgctg ccggccccgc cgatgagcga cgcgaagatc
      301 acctccacgt tccggttcgt gtcgatgaag ctgggcgtga cgtacccgta gttcacgatc
      361 gacagcgcgc cggagaaccc ggcgacgccc gcggaaatcg tgtaggcggc ccacttgtac
      421 cggaaggtgt cgtacccgag gaatttgacg cgcgtctcgt tctccttgat catccgcagc
      481 aggatgccga acggggacgt ggtgagccgc ttcattcccc agcacgtgag gaggagacag
      541 gccaggcaga agccgtacaa cacccgcttg tcctggaaat ccaggaaccc gaccttcgag
      601 aagacggccg acatgccatc ctcccctccg gtgtaccggg caaaggcgat cagcacgagg
      661 aaatgcccga cctggttgaa cgccaggttg atgagcgcga atgccgcgcc cgtcgtccgg
      721 acgacgatgg ggcccagcag cgccccgacc gcggcggcgg ccacgatcgc gaacaggatg
      781 gagaggaacg ggttcggaga gacgtgctct gcgaacagcg tcgcgccgta ggcgccggcg
      841 ccgaggaaca gcatgtgccc gaacgaaagg cggcccatgt atccgtagat gaggtcgaag
      901 gagagcacga aaacgcagaa gatgatgtaa tcggtcgtgc gctggaactt gaggaacggg
      961 aggaggagca ggaacgctcc ggcgatcaga aggatgtcga tccgccgcag cctctcggtc
     1021 acaggttcgt ctccagcgcc ccggtcgcct ctatttcggc tcgggtggcg agctcctgaa
     1081 agatcttccc ctccttcatc acgtagaccc ggtcggcgag ccgcgccacc accggcaggt
     1141 tctgttcgac gatcagcatg gacagacgcc ccttcatctc cgaaaggagc cggaagatgt
     1201 cgtcgatcac tcccgccgcc agcccctcgg tgggctcgtc gaccagcagg atcttcgggt
     1261 tccccgtcag cgcgcgcccg atcaggagga tctgtcgctg ccccccgctc aagcctcccg
     1321 ccttgctgtc gaggaacttc tccatcgcgg gatagatcga gaccatccgc gcgacgacct
     1381 cggacatccg ctctccggac gggtgcgccg ccagctcgag gttctcccgg accgtcaatt
     1441 tcgagaagac gcgcttgtcc tggaagacgt accgcacgcc gaggcgcgcg acgttttccg
     1501 gacggatccc ccgcaggtcc cttccctcga ggcgcacggt gccggccgcg gggggcatga
     1561 atcccgcgat cgtcttgagc aacgtcgtct tccccgcgcc gttgcgtccc gcgatgaaaa
     1621 ccatttcgcc ggggtccacg cgaagcgaca ccttgtggag gacgctcgcg ctcccgtacg
     1681 cggcgtcgac ggcgtcgacc gtgagcaggg acacgctcac ggccgccccc agtacacctg
     1741 ccgcaccagc gggtcggcga gcacctcggt gggaccaccc tcggcgatca gtttcccttc
     1801 gtgcatcacc gacagtctcg acaccaggtc cacgatgcgg gagatcttgt gctcgatgat
     1861 gaccagggtg aattccttcc tcaccctgtg gatcaactcg aggacctcca cgatctcccc
     1921 gtccgagagg ccggcgaacg gctcgtccag caggaggatc gagggccgca ggcagagcgc
     1981 gagggcgatc tccagcttcc gcttgtcgcc gtaggaaagc tccgcggtct tccagagcgc
     2041 cttctcccgg agaccgaccg tccccagcgc cccggcggag agctcggaga ggttcccgct
     2101 ccaggcgtcc tcgaggaaaa aacgggcgga cgccgtctgt tccttcccgg cgcggatcag
     2161 ggcgaggacc aggttctccc gcacggtcag gctgtcgaag accgatacga gctggaaggt
     2221 ccggacgatc ccgcggcgca ccctcccgtg ggcgggaatc cgcgtgatgt cggccccccg
     2281 gtacagcacc gccccctcgg tggggggaaa catcccggtc agcaggttga aaaaggtggt
     2341 cttgcccgcc ccgttgggcc cgatgatccc cgccgactcc ccttcctgga ggcggtagct
     2401 cacaccggac accgcggcga acccgtcgaa ccgcttcgtg acgccccggg tttcgagaat
     2461 gatttcttcg ggcaaggaag gctccctccg acggcgggga gggggaggcg ctccctcccc
     2521 ctcccgccaa tgccgtctag taaccgagag cggaaagggg aggaagataa tcctctcccg
     2581 aatacacgtc cacgatcttg acgaggtccc acttgggatc tttccgttcc ttcgctccct
     2641 tgcccaccac cacgaaggcg ccgtacttga acgtcggctg atgatccgcc cgccacgtcg
     2701 cgggcccctt catggtatcg aaccgggggg atttcatcag cgccgccgcc acggcggcag
     2761 ggtcggtgga ctgcgccaga gcgatcccgc gcatcgcctc gatcgttccg aggtacgcca
     2821 tcgcgctgta cggatcgggg gggtagccgt actccttccg gaacttctgg ctgaagtcgt
     2881 tcgcggcctt gaccaccgcg gcgtccttga acccggtcat gtcgtagtac caggacatga
     2941 gggagtagac cccgtccagg gcgtcggcgg ggacgccgct tccgaagacg ttggtcatcc
     3001 agttgaacca gatcttcgtc tttttcttga gcccgatttc gtaggactgc ttgaggacgt
     3061 tgatcgcatc caccccccag tgggcgagca tcacgatatc gggcttcatc tccgcgacct
     3121 tggtgaggta cggggtgaaa tcgctcgtcc cgaccgggga ttcgaggtat tccgccgtga
     3181 cgccgtactt cttcaaggca tccttcgcgc cggcccactg gtcctgcccg aacgcgtagg
     3241 cgggcccgaa gaagacgatc ttcttgtagc cgaggttctt tacgatgtag gcggccccgg
     3301 cgtaccccgc ggcgtacgcg ctgccgtgga gggcgaaggt cgagggcgcc atctccgcct
     3361 tttcgaacat cttgagcgcg accacgttga ccgggaagta gagcacgggc gtcttcttca
     3421 cttccttgtt cagcgcccct gagatcgggg cgaacgtctg cccgacgatc aggtcgacct
     3481 tctcctccgt caggagctcg cggaaccgcc ggatcgcgat gtccggcttt gactcatcgt
     3541 cccggacgac gaactccaca gggacctttt tcccgccgag cttcacgccg cccgccgcgt
     3601 tgatctcctt gacggccagc ttggcgccgt cgagctgcac cgggccgatc accgaggccg
     3661 ctccggtcat gccgaacatg aaccccacct tgatcttgtc gggcgccgcc gctcccgccc
     3721 ccgcgggcag cagcagcgtc catgccgccg ccagcccgat tcccgccatc gccatccttt
     3781 tcgcaagcct catttccctg cctcctcttc tcgccgtctg ccggcggtgc cttccctatc
     3841 cgagggaggc gagaaattcc ccggatgtca tgactttccc gatcttcggg aagatccact
     3901 tctccgcgta gttctgctcc tccgccgtgt cggtcgccgt gcagtccgag atcacgtgaa
     3961 cccggtaccc cttgtcatag gcggaccgtg ccgacgattc gacgcaccag ttggtgtgga
     4021 accccgtgaa cgcgacgtac tcgatctggt tggcccgcag cagatagtcg agcgccgtcg
     4081 aatggaacgc gtcgagcgtc atcttcccct caacgacgat gtcgcccttc ttcggcgtga
     4141 gctgcttgat gatctcgggg cccggggttc ccttcagcca cgcgccggtt ccccagtcgg
     4201 ccttcatgtc catctcgatc ccgcgcagcc cgtcgcaccc gggtccgccc ttcttcagct
     4261 cggggaaccc
//
LOCUS       DASVJL010000146         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_303459, whole genome shotgun sequence.
ACCESSION   DASVJL010000146 DASVJL010000000
VERSION     DASVJL010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303459"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /gene="typA"
                     /locus_tag="VF357_02925"
     CDS             <1..1593
                     /gene="typA"
                     /locus_tag="VF357_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX7523128.1"
                     /translation="NIVDTPGHADFGGEVERTLKMVDAVLLLVDASEGPLPQTRFVLK
                     KALERELPVILVINKIDRPDARIDEVIDEVYDLFIDLDATEDQLDFPVMMTNARAGTA
                     ALRGEAAGRNLQPLFDAILSHVPPPTGDPSATLQFLVTNLEYSDYVGRLAVGRVFEGT
                     LRAGEIISLCGIGGEVKKLKVALLFGYEGLSRTEITEATAGDIVAIAGMEDVTIGDTI
                     SDAEEPKPLPRIAVDEPTVSMVFSINTSPFAGRDGKFVTSRQVRARLEKELLGNVALR
                     IDFSGTDYFAVMGRGELQLVILIEMMRREGFELSVSRPEVVTKTEGGAVVEPVEALFV
                     DVPDAYLGAVTQALGIRRAKMTKMINPGQGRVRIEYRIPSRGLIGFRSEFLTLTRGTG
                     LLNHLFDGYDPWQGPIPERINGALVSDRVGRTTAYAIFHLQSRGTFFIGEGEQVYEGM
                     IVGENTRPVDMDINITKEKKLTNIRAAGTDEALRLVPHRILSLEVALEFINEDEVVEI
                     TPTSIRMRKKILDAGKRPRRKE"
BASE COUNT          316 a          523 c          562 g          274 t
ORIGIN      
        1 aacatcgtgg atacccccgg tcacgccgac ttcgggggcg aggtggagcg gacgctgaaa
       61 atggtcgacg cggtgctgct gctcgtggac gcctccgagg gtcctcttcc gcagacccgc
      121 ttcgtactca agaaagccct cgaacgggag ctccccgtca tcctcgtcat caacaagatc
      181 gaccggcccg acgcgcggat cgacgaggtg atcgacgagg tctatgacct cttcatcgac
      241 ctcgacgcca cggaagacca gctcgatttc ccggtcatga tgacgaacgc ccgcgcgggg
      301 acggcggctc tccgcgggga agcggcggga cggaatctgc agcccctctt cgacgcgatc
      361 ctctcccacg tgcctccccc gaccggcgac ccgtcggcca ccctccagtt cctggtgacc
      421 aacctcgaat acagcgacta cgtcggacgc cttgccgtcg ggcgggtctt cgaggggacg
      481 ctgcgcgccg gggagatcat ctccctgtgc gggatcggcg gggaggtgaa aaaactcaag
      541 gtggcgctgc tcttcgggta cgaggggttg agccgaacgg agatcaccga ggcgaccgcg
      601 ggggatatcg tggcgatcgc ggggatggag gacgtgacga tcggagacac gatctccgac
      661 gcggaagaac cgaagcccct cccccgcatc gccgtggacg agccgacggt gtcgatggtc
      721 ttctcgatca acacctcgcc gttcgccggg agggacggaa agttcgtcac ttcgcgacag
      781 gtgcgcgccc ggctcgagaa ggagctgctc ggcaacgtcg cgctccggat cgacttttcc
      841 gggaccgact acttcgcggt gatgggacgc ggggaactgc agctcgtgat cctcatcgag
      901 atgatgcggc gggaggggtt cgagctctcg gtatcccggc ccgaagtggt gacgaagacg
      961 gaaggcgggg cggtcgtcga accggtcgag gcgctcttcg ttgacgtccc cgacgcgtac
     1021 ctcggggcag tgacgcaggc gttggggatc cggcgggcga agatgacgaa gatgatcaat
     1081 ccgggacagg ggcgcgtgcg gatcgagtac cgcatcccgt cgcgggggct gatcggcttc
     1141 cgttcggaat ttctcaccct cacccgcggc accggtttgc tgaaccacct cttcgacggc
     1201 tacgatccct ggcaggggcc gatccccgag cggatcaacg gtgcgctggt ctccgatcgg
     1261 gtagggcgca ccacggcgta tgccatcttc cacctgcaat cgcgcgggac gttcttcatc
     1321 ggggaggggg agcaggtgta cgaggggatg atcgtgggcg agaacacgcg tcccgttgac
     1381 atggacataa acatcacgaa ggagaagaaa ctcacgaaca tccgcgccgc cgggacggac
     1441 gaggcgttgc ggctcgtgcc gcaccggatc ctctccctcg aggtggccct cgagttcatc
     1501 aacgaggacg aggtggtcga gatcaccccc acatcgatcc gcatgcggaa gaagatcctc
     1561 gacgccggga agcgcccccg ccgcaaggag tgaccagggg tttcgcttca taaataagat
     1621 ccccacatgc aggaacggaa gcgccaaaag ggccggattt tttcttcctc gggac
//
LOCUS       DASVJL010000147         3064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_584911, whole genome shotgun sequence.
ACCESSION   DASVJL010000147 DASVJL010000000
VERSION     DASVJL010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584911"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VF357_02930"
     CDS             complement(<1..325)
                     /locus_tag="VF357_02930"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7523129.1"
                     /translation="MSRTGSRGMLAAWLREVGVDYVLRPSPGVETLDDIREELTDCRR
                     CPLCAGRATVVFGVGNPRARLMFVGEGPGSEEDRLGEPFVGAAGKRLDRWIARIGLHR
                     EDVYIA"
     gene            complement(322..1524)
                     /gene="coaBC"
                     /locus_tag="VF357_02935"
     CDS             complement(322..1524)
                     /gene="coaBC"
                     /locus_tag="VF357_02935"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX7523130.1"
                     /translation="MTTLSGKEIVLGVTGGIAAYKACEIVRALRKEGAGVRVILTASG
                     ARFITPLTLQTLSKNPVSTDLFDLINESDIGHISLAQRANLLMIAPATANILGKIRSG
                     IADDMLSTVAMATAAPVLLAPAMNSQMYASPAMRENVEVLRERGFTFVEPDDGELACG
                     TVGPGRLADTEKIVEMARILLAEKILAGKRVVVGAGPTAEDIDPVRFLTNRSSGKMGF
                     AMAVVARRFGADVTLVAGPTRLPDPPFMRTVRVRSAKEMMGAVAIAAETADVVVMAAA
                     VADFRPESAVSQKIKKETFDGRIPLVPTEDILASLGHSRGNRVLVGFAAETNDLEGNA
                     VDKMRRKNLDAIVANDVSRPEIGFESDSNEVRVYFSDGTALDLAMAAKDAIASAVWRA
                     VCRKFLPG"
     gene            complement(1521..2177)
                     /locus_tag="VF357_02940"
     CDS             complement(1521..2177)
                     /locus_tag="VF357_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7523131.1"
                     /translation="MTDARSLRVLAMEVGPLAENTYIVGHVASGKAVVIDPGDEADEI
                     LRQLGERGWTLDKILLTHGHFDHVGAVAAIKERTGASVHIHPDDAEQLRTASRQGAMF
                     GLRVPDPPPPDVLVREGDTVPLGDVAFRVLHTPGHTPGHVTYVCGDLAFVGDLIFEGS
                     IGRTDLPGGSLDDLLRAVREKIFTLPGETILFPGHGPATTVGDEKRGNPFFTGEDAVG
                     "
     gene            complement(2174..>3064)
                     /locus_tag="VF357_02945"
     CDS             complement(2174..>3064)
                     /locus_tag="VF357_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523132.1"
                     /translation="FVKSVDARVIKERNYNIVLDYSYGSSAVIFPRILGKLGVETVAL
                     NAILDPARITRTQEEFDRGMGHLAAISRSLSADFGAMLDTGGEKIFLIDEKGDILSDA
                     TALQVVCLLACRQAGQGLVGVPVTASRNVEKIAARFGMDVVRTRTLPRSLMETAAGEG
                     VAFAGGGAGGFVFPRFQPAFDGMFAIVKIMELLAAEGRGLSDILREIPPTVLLHRKIP
                     CAWENKGSLMRMLTGYAKGKPSQFIDGVKVFEGEDWALVYPSQDEAYFHLVVESGDGR
                     AAQRLASEYTDLFASWGEKL"
BASE COUNT          471 a         1124 c          983 g          486 t
ORIGIN      
        1 tggcgatgta gacgtcctcc cgatgaaggc cgatccgcgc gatccaccga tcgagccgct
       61 tcccggcggc cccgacgaac ggctccccca ggcggtcctc ctcggacccg ggtccctcgc
      121 cgacgaacat cagccgggcg cgggggttcc cgaccccgaa gacgaccgtg gcgcgccctg
      181 cgcagagcgg acacctgcgg cagtcggtca gctcctcgcg gatgtcgtcg agggtctcaa
      241 cgcccggcga cggccggagg acgtagtcca ccccgacctc ccggagccac gctgcaagca
      301 tcccgcggga accggttcgg ctcatccggg gaggaacttc cggcagaccg cccgccagac
      361 ggcggaggcg atcgcgtcct tggccgccat ggcgaggtcg agcgcggtcc cgtcggagaa
      421 gtagacgcgt acctcgttgc tgtcggactc gaacccgatc tcgggccgtg agacgtcgtt
      481 cgccacgatc gcgtcgagat tcttccggcg catcttgtcg accgcgttcc cctcaaggtc
      541 gttcgtctcc gccgcaaacc cgacgagcac acggttcccc ctggagtgcc cgagggaggc
      601 gaggatgtcc tccgtgggaa ccagcgggat ccgtccgtcg aacgtctcct tcttgatttt
      661 ttgcgacacc gcggactccg ggcggaagtc ggccaccgcc gccgccatca ccaccacgtc
      721 cgccgtttcc gctgcgattg ccaccgcccc catcatctcc ttcgcggacc gcacccggac
      781 cgttctcatg aacgggggat ccggcagccg ggtcggtccc gcgacgaggg tcacgtccgc
      841 gccgaatcgc cgggcgacga ccgccatggc gaaccccatc ttgccgctgg accggttcgt
      901 gaggaaccgg accgggtcga tgtcctccgc cgtgggtccc gcccccacca cgaccctctt
      961 ccccgcgagg atcttctccg ccaggagaat ccgggccatc tcgacgatct tctccgtgtc
     1021 cgccagccgc cccgggccca ccgtgccgca ggcgagctcc ccgtcgtcgg gttccacgaa
     1081 ggtgaacccc cgctcccgga ggacctccac gttttcccgc atcgccgggg aggcgtacat
     1141 ctggctgttc atcgcaggcg cgaggagtac cggcgcggcg gtggccatcg ccacggtcga
     1201 gagcatgtcg tccgcgatgc cgctccggat cttcccgagg atgttcgcgg tggccggtgc
     1261 gatcatcagg aggttcgccc gctgcgcgag ggagatgtgg ccgatgtcgg actcgttgat
     1321 gaggtcgaac aggtcggtag agaccgggtt cttcgaaagg gtctgcaggg tgagcggggt
     1381 gatgaagcgc gccccggagg cggtgaggat cacccggacg cccgcgccct ccttgcgcag
     1441 cgcgcggacg atctcgcacg ccttgtacgc cgcgatcccc ccggtgaccc cgaggacgat
     1501 ctccttgccc gagagcgtgg tcatccgact gcgtcctccc ccgtgaagaa tgggttcccg
     1561 cgcttttcat cgcccacggt cgtggccggc ccgtgccccg gaaaaagaat ggtctccccg
     1621 ggcagcgtga agatcttctc ccggacggcg cgcagcaggt cgtcgaggga gcccccggga
     1681 agatccgtcc ggccgatgga cccctcgaag atgagatcgc ccacgaaggc gaggtccccg
     1741 caaacgtacg tcacgtgccc cggggtgtgc cccggggtgt ggagaacgcg gaacgccacg
     1801 tctcccagcg gaacggtatc gccctcccgc acaagcacgt cggggggcgg agggtcgggg
     1861 acgcgcagcc cgaacatcgc accttgccgg ctcgccgtcc gcagctgttc cgcgtcgtcc
     1921 ggatggatgt ggacgctcgc acccgtgcgc tccttgatgg ccgcgaccgc ccccacatga
     1981 tcgaagtgcc cgtgggtgag aaggatcttg tccagggtcc atccccgctc gcccaactgg
     2041 cgcaggatct cgtcggcctc gtctccgggg tcgatcacga ccgcctttcc gctcgccacg
     2101 tgccccacga tgtaggtgtt ctccgccagc gggccgacct ccatcgccag caccctgagc
     2161 gaccgggcgt cggtcacagc ttctcccccc aggacgcgaa caggtccgtg tattccgagg
     2221 cgagccgctg cgccgcgcgg ccgtccccgg attcgaccac caggtggaag tacgcctcgt
     2281 cctggctcgg gtagaccagc gcccagtcct ccccctcgaa caccttcacc ccgtcgatga
     2341 actggctcgg cttccccttg gcgtacccgg tcagcatccg catgagggac cccttgttct
     2401 cccaggcgca ggggatcttc cggtggagca ggacggtcgg cgggatctcg cggaggatgt
     2461 cggacagtcc cctcccctcg gccgccagca gttccatgat cttcacgatc gcgaacatgc
     2521 cgtcgaacgc cggttggaag cgcgggaaga caaacccgcc cgccccgccc cccgcgaaag
     2581 caaccccctc ccccgccgcc gtttccatca gtgaccgcgg cagcgtccgc gtccggacca
     2641 cgtccatacc gaagcgcgcc gcgatcttct cgacgttccg cgaggcggtg acgggaactc
     2701 ccaccagccc ctggcccgcc tgacggcagg cgaggaggca gaccacctgc agcgccgtcg
     2761 cgtcggagag gatgtccccc ttctcgtcga tcaggaagat cttctcgccg ccggtgtcga
     2821 gcatcgcgcc gaagtcggcg gacagggagc gcgagatcgc cgcgagatgg cccatccccc
     2881 tgtcgaactc ctcctgcgtc cgggtgatgc gggccgggtc gaggatggcg ttcagcgcca
     2941 ccgtctccac cccgagcttt ccgaggatcc gcgggaagat gacggcggag gaaccgtagg
     3001 agtagtcgag cacgatgttg tagttccgct ccttgatcac ccgcgcgtcg acggatttca
     3061 cgaa
//
LOCUS       DASVJL010000148         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_772848, whole genome shotgun sequence.
ACCESSION   DASVJL010000148 DASVJL010000000
VERSION     DASVJL010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772848"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VF357_02950"
     CDS             <1..360
                     /locus_tag="VF357_02950"
                     /inference="COORDINATES: protein motif:HMM:NF019005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030701 - NAD+-dinitrogen-reductase
                     ADP-D-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--dinitrogen-reductase
                     ADP-D-ribosyltransferase"
                     /protein_id="HEX7523133.1"
                     /translation="RTNAVEAQFDLLYEFVQYELRRRHPGVPSFTLYRGVRNLADYRV
                     LENPGGNRAVVRLNNLNSFSSRIEHAWQFGGNVFEADVPAAKIFFRADLLPGALPRGE
                     EEAMVIGGDFEVTVRSY"
     gene            357..1283
                     /gene="draG"
                     /locus_tag="VF357_02955"
     CDS             357..1283
                     /gene="draG"
                     /locus_tag="VF357_02955"
                     /EC_number="3.2.2.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047407 - ADP-ribosyl-[dinitrogen
                     reductase] hydrolase activity [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyl-[dinitrogen reductase] hydrolase"
                     /protein_id="HEX7523134.1"
                     /translation="MSPRRKAPPGAAGIVNRARAALLGGAVGDALGATTEFMTPAQIR
                     DRFGVHREIVGGGWLKLPPGQVTDDTEMTLCVARGIAEPGRWDLTEIADRFARWLSGG
                     PVDVGATCRRGIEEYMEKERLEAPPDEQGAGNGAAMRVAPVALYTLGDEELLSRLAVE
                     QAHITHHHPLSDAACVSVGKMIQRGLLGAPLRDLQTTAEELAAQHPEFRFEGYDGKSS
                     GYVVDTLRTVFDAFFSADNFEDCVVKTVNRGGDADTTGSIAGAIAGARYGLAAIPRRW
                     LQALDGSLRKELHLLAGDLVRKSPLFAGTSPR"
     gene            1312..2205
                     /locus_tag="VF357_02960"
     CDS             1312..2205
                     /locus_tag="VF357_02960"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7523135.1"
                     /translation="MELDPELTAQLKRVLSSLETLLPRPVPRIDWEECVAANWRKRSF
                     SGYLEPVGNVENLHLEDLLGIEEQKRVVEENTRQFLAGLPANNILLWGSRGTGKSSLV
                     RALLHAYAPLGLRIIQVDKDDLVHLPSIVDEVKGLPYKFLLFSDDLSFETGESSYKML
                     KSALDGSVYAPPENVLIYVTSNRRHLVPEYESDNRGAMMADGEIHHGEAVEEKISLSG
                     RFGIWVAFHPFSQDLYIEVVRRWVEKMCGKHGAHLPWTKEARDEAILWSQRKGDRSGR
                     IAYQFAGHWVGKELLRREPVA"
BASE COUNT          419 a          753 c          800 g          373 t
ORIGIN      
        1 cggacgaacg ccgtggaggc ccagttcgac cttctctacg agttcgtcca gtacgaactg
       61 cgacggcggc atccgggagt cccctccttc accttgtacc ggggagtccg caacctcgcg
      121 gattatcggg ttctcgagaa ccccggcggg aaccgcgccg tggtccggtt gaacaacctc
      181 aactccttct cctcccggat cgagcacgcg tggcagttcg ggggaaacgt cttcgaggcc
      241 gacgtccccg ccgccaagat cttcttccgc gcggacctcc ttccgggcgc ccttcccagg
      301 ggcgaggagg aggcgatggt gatcggcggg gatttcgaag tgaccgtgag gagctattga
      361 gcccgcgaag gaaagccccc cccggcgcgg ccgggatcgt gaaccgggcg cgcgccgccc
      421 tcctgggcgg tgccgtgggg gacgcgctgg gcgccacgac cgagttcatg acgccggcgc
      481 agatccggga ccggttcggc gtccaccgcg agatcgtcgg cggcggctgg ctgaaactgc
      541 ctcccggcca ggttaccgac gacacagaga tgacgctgtg cgtcgcccgg gggatcgcgg
      601 agcccggccg ttgggacctc acggagatcg ccgaccggtt cgcccgatgg ctttccggag
      661 gtcccgtcga tgtgggggcc acctgccgtc gcgggatcga ggagtatatg gaaaaagaac
      721 ggcttgaagc gccccccgac gagcaggggg cgggaaacgg cgcggcgatg cgggtcgctc
      781 cggtcgcgct ctacacgctc ggggacgagg aacttctctc ccgcctcgcg gtcgaacagg
      841 cgcatatcac ccaccaccac ccgctgtccg acgcggcgtg cgtgtcggtt ggaaagatga
      901 tccagcgggg ccttctcggc gcgcccctgc gcgacctgca gacgacggcg gaagaattgg
      961 ccgcacaaca cccggagttc cgtttcgagg ggtatgatgg caagtcgtcc ggatacgtgg
     1021 tggacacgct tcggacggtg ttcgacgcct tcttctccgc ggacaacttc gaggattgcg
     1081 tcgtgaaaac ggtgaaccgg gggggcgacg cggacacgac ggggtccatc gccggcgcga
     1141 tcgcgggcgc ccggtacgga ctcgccgcga ttccgcgaag atggctgcag gcgcttgacg
     1201 ggagcctccg caaggaactt cacctcctcg cgggggacct cgtccggaaa tcgcccctgt
     1261 tcgcggggac gtcaccgcgt tgagatcaag atcacgacga caggagtgga catggaactg
     1321 gacccggaac tgaccgcgca actgaaacgg gtgctttcct ccctcgagac gttgctgccg
     1381 cgccccgtgc cccggatcga ctgggaggag tgcgtcgccg cgaactggcg gaagcgctcc
     1441 ttctcggggt acctcgaacc ggtcggaaac gtcgaaaacc tccacctgga ggatctgctc
     1501 ggcatcgagg agcagaaacg ggtggtggag gagaacaccc ggcaattcct cgccggcctc
     1561 cccgcgaaca acatcctcct gtggggttcg cgcggcaccg ggaaatcgtc gctcgtgcgg
     1621 gcgctcctcc atgcgtacgc ccccctgggg cttcggatca tacaggtgga caaggacgac
     1681 ctcgtccacc ttccctccat cgtggacgaa gtgaaagggc tcccctacaa attcctcctc
     1741 ttctccgacg acctctcctt cgagacgggg gagtcgagct acaagatgct gaagagcgcc
     1801 ctcgacggct cggtgtacgc accgccggaa aacgtcctga tctacgtgac gtccaaccgg
     1861 cggcacctgg tccccgagta cgagagcgac aaccgcggcg cgatgatggc cgatggagag
     1921 atccaccatg gggaggccgt ggaggagaag atctcccttt cgggacggtt cggaatctgg
     1981 gtcgccttcc acccgttctc gcaggacctg tacatcgagg tggtacggcg ctgggtggag
     2041 aagatgtgcg gaaaacacgg cgcacacctc ccatggacga aggaggcgcg ggacgaggcg
     2101 atcctatggt cccagcggaa gggggaccgg agcggccgca tcgcttacca gttcgccggc
     2161 cactgggtcg ggaaggagct gctccgccgg gaacccgtcg cgtagcgcct cgcgatataa
     2221 tagagggaga gacgacggtg ggaggtgcgc cgtgccggtc ccgaaggata tcgtgcggat
     2281 ctccgatacg gtatgggaga tacccgtctc ccacaaggcg gggatgctcg taccggcccg
     2341 catct
//
LOCUS       DASVJL010000149         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1805523, whole genome shotgun sequence.
ACCESSION   DASVJL010000149 DASVJL010000000
VERSION     DASVJL010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805523"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /gene="selA"
                     /locus_tag="VF357_02965"
     CDS             <1..1199
                     /gene="selA"
                     /locus_tag="VF357_02965"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX7523136.1"
                     /translation="IASRESLPMRRVINAAGIVVHTNLGRAPLPGEALAAVLETARGY
                     SNLEFDLEAGERSSRLSHVEGMLRALTGAECAHVVNNNAAAVLLCLAGHARGREVIVS
                     RGELVEIGGSFRIPDIMAESGARLVEVGTTNRTRLSDYENAVTGQTALLLKVHPSNFS
                     IHGFTEEVRTADLARLGERAGIPVMEDQGSGALIDLAAHGIPGAVSLRQALSAGPGIV
                     TASGDKLLGGPQAGIILGKEDLVAPLKKHPLSRALRVDKMCLAALSAVLRLYADERRA
                     RDRVPVLRMLLEPEERVRARARRLVRAIRAAGAELSLAIERGDTSPGGGALPDIYLPT
                     ALVAVSHPRIPEAVLEERLRRGTPRVVARVGGGRVLLDLRTVRNDEVPELAAAVSAID
                     RPPYDG"
     gene            1221..1829
                     /locus_tag="VF357_02970"
     CDS             1221..1829
                     /locus_tag="VF357_02970"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX7523137.1"
                     /translation="MGCPMLNSSVLVLNRGYFPVHVTNARRAFCLLYSGLARAINGQY
                     ETFDYPSWSALAVAAGDPAIGVVGKVVRIPRVVVLVAYDRVPRRNVRFSRRNIFVRDR
                     NTCQYCGRPFPSSELNLDHVVPRSQGGKTNWENIVCSCIPCNKRKGGNRPEETGMRLV
                     SAPRTPRWSPEFAFSLRTPIHREWVPFLNVVDFTYWNLELQD"
     gene            1826..2701
                     /gene="aroE"
                     /locus_tag="VF357_02975"
     CDS             1826..2701
                     /gene="aroE"
                     /locus_tag="VF357_02975"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX7523138.1"
                     /translation="MNPQTARGRGAASPSLLFVVGHPISHSLSPAMHNGVIARLGLPL
                     HYVPVDLPPGHLRGFLRIVRAGNFLGGNVTIPYKEEAAALADIRSEAVEVCGAANTLV
                     VRKGKLHAENTDGDGFLDALEAVGWGRRFRRVVLLGAGGAARGIAFALGRAGAREIVL
                     LNRHPRRAERVARLLSGRYPAIVFSGGDLRTETLREEFRGADLIVQCTSLGLQGEWEN
                     FPIKDVQKSSGFADIVYRAGGTNLVRRLKARGVRTVGGLPMLAYQAARSFSLWTGRKV
                     PGEAFRTLAVKALKL"
     gene            2739..>2943
                     /locus_tag="VF357_02980"
     CDS             2739..>2943
                     /locus_tag="VF357_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523139.1"
                     /translation="MNMSVLTNKIGEMLLKGNLISADQLRGALETQEKTHERIGTVLV
                     KAGFIKEEELLAFLGRQFNLPVVD"
BASE COUNT          483 a          981 c          986 g          493 t
ORIGIN      
        1 agatcgcgtc aagggaatcc ctcccgatgc gccgggtgat caacgccgcc gggatcgtcg
       61 tccacaccaa cctcgggcgt gcgccgttgc ccggagaggc gcttgccgcg gtcctcgaaa
      121 ccgcacgggg gtattcgaac ctcgagttcg acctcgaggc gggggaacga tcgtcccgcc
      181 tctcgcacgt cgagggtatg cttcgcgccc tgaccggcgc cgaatgcgcc cacgtcgtca
      241 acaacaacgc cgccgcggtg ctcctctgtc tcgcgggcca tgcccggggg cgggaagtga
      301 tcgtcagccg cggcgagctc gtcgagatcg gcggatcgtt ccggatcccc gacatcatgg
      361 cggagagcgg cgcccgcctg gtagaggtgg ggaccacaaa ccggacgcgc ctttccgatt
      421 acgagaacgc cgtcacgggg cagaccgcgc tcctgctcaa ggtacacccg tccaacttct
      481 ctattcacgg gtttaccgag gaggtccgca ccgccgatct cgcccgtctg ggcgagcgcg
      541 cgggaatccc cgtcatggag gaccagggat cgggggcgtt gatcgacctc gcggcgcacg
      601 gcattcccgg ggcggtgtcc ctgcggcagg ccctgtccgc cggtcccggg atcgtcacgg
      661 cgagcgggga caagctcttg gggggaccgc aggcggggat catcctcgga aaggaagacc
      721 tggtcgcgcc gttgaagaag cacccgctct cccgggcgct ccgggtcgac aagatgtgcc
      781 tcgccgcgct ctcggcggtc cttcgtctct atgcggatga acgccgggcg cgggaccggg
      841 ttcccgtcct ccggatgctc ctcgaacccg aggaacgggt gcgcgccagg gcgcgccgcc
      901 tcgtacgggc gattcgcgct gcgggggcgg aactatccct cgccatcgag cgtggcgata
      961 cctctcccgg cggtggcgcc cttcccgaca tctaccttcc gaccgccctc gtcgccgttt
     1021 cgcacccgcg gattccggaa gcggtcctcg aagagcggtt gcgccggggg acgccccgcg
     1081 tggtcgcccg tgtaggggga gggagggtgc tcctcgatct gcggaccgtc cggaacgatg
     1141 aggttccgga gctagccgcg gccgtgtctg ccattgaccg acccccgtac gatgggtaga
     1201 gtaggagaca caatgaatcc atgggctgcc cgatgctgaa ctccagcgtc ctcgtcctca
     1261 accgggggta cttcccggtc cacgtcacga acgcgcgccg ggccttctgc ctgctgtact
     1321 cggggcttgc ccgggcgatc aacgggcaat acgagacgtt cgactacccg tcgtggagcg
     1381 ccctcgcggt ggccgccggg gatccagcca tcggggtcgt ggggaaggtg gtccggatcc
     1441 cccgagtggt ggtcctcgtc gcgtacgacc gggttccgcg gcgcaacgtc cggttcagcc
     1501 gtcgcaacat cttcgtccgg gaccgcaaca cctgccagta ttgcgggagg ccgttcccca
     1561 gcagcgaact caacctggac cacgtggtgc cccgttccca ggggggaaag accaactggg
     1621 agaacatcgt ctgcagctgc atcccctgca acaagcggaa aggcgggaat cgacccgagg
     1681 agaccgggat gcggctggtc tccgctccga ggacgccccg ctggtcgccg gagttcgcct
     1741 tctccctgcg aactcccatc caccgggaat gggttccgtt cctcaacgtg gtcgacttca
     1801 cgtattggaa cctcgaactc caggattgaa cccgcaaact gcaaggggga ggggcgcggc
     1861 ctccccttcc ctcctcttcg tggtcgggca cccgatctcg cattccctta gccccgcgat
     1921 gcacaacggc gtcatcgccc ggctgggcct tcctctccac tacgttcccg tcgatctccc
     1981 ccccggacat ctccgcgggt tccttcggat cgtacgcgcg gggaacttcc tcggggggaa
     2041 cgtcacgatc ccctacaaag aggaagccgc cgctctggcg gacatccgtt cggaggcggt
     2101 ggaggtctgc ggcgcagcga acaccctggt ggtccggaaa gggaaactcc atgcggagaa
     2161 cacggacggg gacgggttcc tcgacgccct cgaagcggtg gggtggggac gtcgattccg
     2221 ccgggtcgtc ctcctcgggg ccggcggggc ggcccgcgga atcgccttcg ccctcgggag
     2281 ggcgggcgcc cgggagatcg tcctgctgaa ccggcacccc cgcagggcgg aacgcgtcgc
     2341 gcggctcctt tcaggtcggt atcccgcgat cgtcttttcc ggcggtgatc tccggaccga
     2401 aaccctccgg gaggagttcc ggggggcgga cctcatcgta cagtgcacct ccctcggcct
     2461 ccagggggag tgggaaaatt ttccgataaa agatgtacag aaatcgtccg gcttcgccga
     2521 tatagtctac cgggcagggg gaacgaacct ggtccggcgg cttaaggcac ggggcgtaag
     2581 gacggtggga gggctcccga tgctggcata ccaggccgcg cggagcttct ccctttggac
     2641 cgggcggaag gtgccggggg aagcgttccg gacgttggcg gtaaaggctt tgaagttatg
     2701 atacattcga ccgttatccg gatagttcgg aggtaggggt gaacatgtcc gtactgacca
     2761 acaagatcgg cgagatgctc ctgaaaggga acctgatttc cgccgaccaa ctgcgcggag
     2821 ccctcgaaac ccaggagaaa acgcacgaac ggatcggaac cgtcctggtg aaggccggct
     2881 tcatcaagga agaggagctc ctcgccttcc tcggccgtca gttcaacctc cccgtcgtcg
     2941 acc
//
LOCUS       DASVJL010000150         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1055220, whole genome shotgun sequence.
ACCESSION   DASVJL010000150 DASVJL010000000
VERSION     DASVJL010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055220"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VF357_02985"
     CDS             <1..330
                     /locus_tag="VF357_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7523140.1"
                     /translation="QVLTNVVKNAVEAAPERDGKVLVTLSEWDGGVRITVEDNGLGMT
                     AEELGKVFQPFYTNKAQGVGLGMPIVKRLVELHGGEVRIDSGAGRGTTVSVDLPRYPF
                     AGTGGAQ"
     gene            327..1736
                     /locus_tag="VF357_02990"
     CDS             327..1736
                     /locus_tag="VF357_02990"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7523141.1"
                     /translation="MKRKILLVDDEEIFLRPLSRTIVAAGFLVDTAGTATEAREKLKN
                     GEFDLVISDVRMPGVDGIVFTRALREEYGDLPVILMTAYGSIRTAVDAMKAGAQDYLL
                     KPFEPDEILLHIHRILEIQDLRAADRFRTARNQETFDLRKTLVFESPAMQRLQREMTG
                     VLKLDTTILLTGETGTGKQLIARAIHYNGPRRDGPLIEVNCAAIPETLFETELFGHAR
                     GAFTGAVTDRKGMFQLAHGGTLFLDEIGEMPLSLQPKLLTAIQEKKFLRVGGTRQIDV
                     DVRIIAATNKSLEEEVAAGRFRKDLFFRLSVFPIRVPPLRERSEDLPVLGDFFLRRFA
                     RKCGKEIGPLSADDLSALHAYDWPGNVRELENFIERAVIRAAGDRAEIAATLGGSTLE
                     PAGEGSDLPFHPGLPFRDAKERVMSAFEKRYIEDALRRAAGKLTEAARLAGMDNKNFS
                     EKMKRHGVTLENFKMSPPN"
BASE COUNT          331 a          549 c          604 g          283 t
ORIGIN      
        1 caggtgctga cgaacgtcgt gaaaaacgca gtcgaagcgg ccccggagcg ggacggcaag
       61 gtgcttgtca cgctttcgga gtgggacggc ggcgtccgga tcaccgtaga ggacaacggc
      121 ctcgggatga ccgccgagga gctggggaaa gtcttccagc cgttctacac gaacaaggcg
      181 cagggggtgg ggctggggat gccgatcgtg aagcgcctgg tggaactgca cggcggcgag
      241 gtccggatcg actccggggc cggcagggga acgacggtct ccgtggatct gccccggtat
      301 ccgttcgccg gcacgggagg ggcgcaatga agcggaaaat cctcctggtg gacgacgagg
      361 agatcttcct ccgccctctc tcgcgcacga tcgtcgccgc cgggtttctc gtggacacgg
      421 cgggaacggc caccgaggcg cgggagaagc tgaaaaacgg cgagttcgac ctcgtcatca
      481 gcgacgtccg gatgccggga gtggacggaa tcgtcttcac ccgcgcgctg cgggaggagt
      541 acggcgacct cccggtgatc ctgatgaccg cctacggcag catccggacc gccgtcgacg
      601 cgatgaaggc cggggcgcag gattacctgc tgaagccgtt cgagccggac gagatccttc
      661 tccacatcca tcggatcctg gagatccagg acctgcgggc cgcggaccgg ttccggacgg
      721 cgcgcaacca ggagacgttc gacctgcgca agacgctcgt cttcgagagc ccggcgatgc
      781 aacggctcca gcgggagatg acgggcgtac tgaagctgga caccacgatc ctcctgacag
      841 gagagacggg gacggggaag cagttgatcg cacgggcgat ccattacaac ggcccccggc
      901 gcgacggacc gctgatcgag gtcaactgcg ccgccatccc cgaaacgctc ttcgagacgg
      961 agctgttcgg ccacgcccgc ggggcgttca cgggtgccgt caccgaccgg aaggggatgt
     1021 tccagctcgc ccacgggggg acgctcttcc tcgacgagat cggcgagatg ccgctctcgc
     1081 tgcagccgaa gctgctcacg gcgatccagg agaagaagtt cctgcgcgtc gggggaaccc
     1141 ggcagatcga cgtcgatgtg cggatcatcg ccgccacgaa caagtccctc gaggaggagg
     1201 tcgccgcggg gcgcttccgc aaggatctct tcttccgtct ctcggtcttc ccgatccgcg
     1261 ttcccccgct gcgggaacga tccgaagacc tcccggtcct cggagacttc ttccttcggc
     1321 ggttcgcccg caagtgcggg aaggagatcg gccccctgtc ggcggacgac ctgtccgcgc
     1381 ttcacgcgta cgattggccg gggaatgttc gggaactgga gaatttcatc gagcgcgccg
     1441 tcatccgtgc ggcaggagac cgggccgaga tcgcggcgac gctgggcgga tcgaccctgg
     1501 agccggccgg tgaggggagc gatcttccct tccacccggg tcttccgttt cgcgatgcga
     1561 aggagcgcgt gatgtccgcg ttcgaaaagc ggtatatcga ggacgccctg cgccgcgcag
     1621 cggggaaact gaccgaagcc gcccgcctcg ccgggatgga caataagaac ttttcggaga
     1681 agatgaaacg gcacggagtt accctcgaga atttcaaaat gtcccctccc aattaatttc
     1741 agatatgtaa ttattctccc caggttg
//
LOCUS       DASVJL010000151         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_117759, whole genome shotgun sequence.
ACCESSION   DASVJL010000151 DASVJL010000000
VERSION     DASVJL010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117759"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="VF357_02995"
     CDS             <1..1318
                     /locus_tag="VF357_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7523142.1"
                     /translation="ASAMESVDFREAVNRALSNNAFVSAAGEDAVAARRDADAARGYL
                     LPSVRFDEKFVRTTIPAEAFGLKMNQEKLLATDFADVRNFNSPPPRNDFIATLSLEQP
                     LFAPKAYIGYGMAKVEADAKGQDLYRRKEDAIYRVLTAVLDVITARQFVEVAGQGFSD
                     AREHLRIAESLEAAGMGLSSDALRAKVAVASAESAKVTAENRLELARRRLALAMGEPG
                     ARPVDVTGPPPEFPDPGMPGDRETGTIDRADLRASSLRVANAGSNVRLRRSGYLPDVG
                     LSAAYQVDAEDSPLSSDNRSWKVGVGLTWNIFDGMRREAEVGKAVASLRRAQAYHRGM
                     RDQAAFEAAQADLGVKEATLRGEIARAALASAEEGVRLLKSRYENHLGRMVDLLDAQT
                     ALDGARADRIRAENDVRLSRAQRLYASGGLLSFAAPSEEYGKEKNR"
     gene            1315..>2284
                     /locus_tag="VF357_03000"
     CDS             1315..>2284
                     /locus_tag="VF357_03000"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX7523143.1"
                     /translation="MNGVTRLSSFVARSASAAVILSMVAGFSACGEKGKQGTAVSRPV
                     VQGVEVVVVRPVPRETMAEALGTVRARTTAAVAPQVMGRLTGVAVSEGSVVAAGALLA
                     TIDDTTVRAQLSSAEGAVAEAEAAREEVDRAISQAEAGKALAEKTYERFRKLLEGKVV
                     TQQEFDEVDMRRTVAVKDLERAQQKRAQVSAKIAQAKGQADAAKAMLAWTRVTAPFAG
                     AIVEKRADAGSMAIPGVPLFFLEDPRRHRIEVSVSETYLPFLKNGTPVQGILDADPGK
                     PFSAVITEIDPAIDPASRTFTVKADLPAGRARSGQSGKVRFAAGKGT"
BASE COUNT          369 a          714 c          868 g          333 t
ORIGIN      
        1 cgcgtcggcg atggagtcgg tcgacttccg cgaagcggtg aacagggcgc tttcgaacaa
       61 cgcctttgtc tcagcggcgg gagaggatgc cgtcgccgca cgccgcgatg cggacgcggc
      121 ccgcgggtac cttctccctt ccgtccggtt cgatgaaaaa ttcgtccgca cgaccattcc
      181 cgcggaggcg ttcgggctca agatgaacca ggagaagctg ctcgcgacgg atttcgccga
      241 tgtgcgcaac ttcaacagcc ctccgccgcg caacgacttc atcgccaccc tttcgcttga
      301 gcagccgctt ttcgccccga aggcgtacat cgggtacggg atggcgaagg tggaggcgga
      361 cgcgaagggc caggacctct accggcggaa ggaggatgcg atctaccggg tcctcaccgc
      421 ggtcctcgac gtcatcacgg cccggcaatt cgtcgaggtt gcgggccagg ggttctccga
      481 cgcccgggag cacctgcgga tcgcggagag cctcgaggcg gcggggatgg gactctcttc
      541 cgatgctttg agggcgaagg tggcggtcgc ctccgcggaa agcgcgaagg tgacggcgga
      601 gaaccggttg gagctggcgc gacgcagact cgcccttgcg atgggggagc cgggggcgcg
      661 ccctgtggat gtgacgggcc cgcccccgga gttccccgat cccgggatgc ccggtgatag
      721 agagaccgga acgatcgacc gggccgacct gcgcgcctcg tcgctccggg tcgcgaacgc
      781 ggggagcaat gtccggctgc ggcggtcggg atacctgccc gacgtcggcc tttcggcggc
      841 gtatcaggtg gacgccgagg attcccccct ctcctccgac aaccgctcct ggaaggtggg
      901 cgtgggactg acctggaaca ttttcgacgg gatgcgccgc gaggcggagg tggggaaagc
      961 cgtcgcttcg ctccggaggg cgcaggcgta ccaccgcgga atgcgggacc aggcggcgtt
     1021 cgaggcggcg caggcggatc tgggtgtgaa ggaagcgacg cttcgcggag agatcgcccg
     1081 cgccgccctc gcgtcggcgg aggagggcgt gcggctgctc aaatcccggt acgagaacca
     1141 cctcggcagg atggtggacc tcctcgacgc gcagacggcc ctcgacggtg cccgcgccga
     1201 ccggatccgc gctgagaacg acgtgcggct ctcccgcgcg cagcggttgt acgcctccgg
     1261 cgggctgctc tccttcgccg cgccgtcgga ggaatacggg aaggagaaaa accggtgaac
     1321 ggcgtgacga ggttgtcttc cttcgtggcg cgatccgcat cggcggccgt gatcctttcg
     1381 atggtcgcgg ggttttccgc ctgcggagaa aaaggaaagc aggggacggc ggtatcccgc
     1441 cccgtggtgc agggggtgga ggtggtcgtc gtccggccgg tcccgcggga aacgatggcg
     1501 gaagcgctgg ggacggtccg ggcgcggacg accgccgcgg tggctccgca agtgatgggg
     1561 cggctgaccg gcgtcgcggt ttcggaaggt tcggtggtgg cggcgggggc gctcctggcg
     1621 acgatcgacg acacgacggt ccgcgcgcag ctctcctcgg cggaaggggc ggtcgcggaa
     1681 gccgaggcgg cacgcgagga ggtcgaccgg gccatctccc aggcggaggc cgggaaagcc
     1741 ctcgcggaga agacgtacga acggttccgg aagctgctgg aggggaaggt ggtcacgcaa
     1801 caggagttcg acgaggtcga tatgcggcgc accgtggccg tgaaggatct cgagcgggcg
     1861 cagcagaaga gggcgcaggt gagcgcgaag atcgcccagg cgaaagggca ggccgatgcg
     1921 gcgaaggcga tgctcgcctg gacacgggtg accgccccct tcgcgggggc gatcgtcgag
     1981 aagcgggccg acgcggggtc gatggccatc cccggcgttc ccctcttttt cctggaggac
     2041 ccgcgccgtc accgcatcga ggtgtccgtc tccgagacat acctaccgtt tctgaagaat
     2101 ggaaccccgg tccaggggat cctggacgcc gaccccggaa agccgttttc ggccgtcatc
     2161 acggagatcg atccggcgat cgaccccgcg agccgcacct tcacggtgaa ggcggatctc
     2221 ccggcgggcc gggcccggtc gggccagtcc ggcaaggtcc gcttcgccgc gggaaagggg
     2281 acgg
//
LOCUS       DASVJL010000152         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1617170, whole genome shotgun sequence.
ACCESSION   DASVJL010000152 DASVJL010000000
VERSION     DASVJL010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617170"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1235)
                     /locus_tag="VF357_03005"
     CDS             complement(54..1235)
                     /locus_tag="VF357_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7523144.1"
                     /translation="MSLFLLSFFLIYGSMHAYALLKARSALAFAPGTTLSLLLLLGIL
                     LCAPILTFSLSRHGYEEAARIVAYVGYLWMGFLFFFTCLNLSVDLLRLPLLAMGRGGI
                     AATTAGGLAGRPAFLCVAGLAVALSAYAIVEASRIQVVRVRIVTDRLPASVPSLRIAQ
                     ISDLHLGLIHRNGKAREVAAIVAREHPDIFVSTGDLVDGQLDGVAELEEILRGIPAPR
                     GKFAALGNHEYYAGIDRSIAFTRKSGFTVLRDESVTIDDAVRIAAVDDPAAARFGRTG
                     GTDEAALLGNRPDGRFTLLLKHRPQLDPATGGKFDLQLSGHTHDGQIFPFRLLTRLAF
                     PLLGGDHLVPGGGILHVSRGTGTWGPPMRFLAPPEVTIVEIERTPAAPGDVSTPVGLN
                     P"
     gene            1289..1558
                     /locus_tag="VF357_03010"
     CDS             1289..1558
                     /locus_tag="VF357_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7523145.1"
                     /translation="MPTYEYKCPACGEFEKEQRMSAPPLKKCPHCGKAVTRLVGGGGG
                     FVLKGGNWVSKMSSSGESAKKKSDRFMKQTVGEVAEDIAKHSRSH"
     gene            complement(1555..2286)
                     /locus_tag="VF357_03015"
     CDS             complement(1555..2286)
                     /locus_tag="VF357_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX7523146.1"
                     /translation="MCRMIAFSSNEPRDVAPYLASLARFCESGNLVAGWERRPGGNHP
                     NGWGVAWRAGEEIRMVKSGSPAASDPLLFGLRAVTDRFLGHVRYASNPETVCAANSHP
                     FQALGVTLAHNGTFYGKIGAEGDARKVSDTVVFLELLEDRWVERTLEGLSEALRGILS
                     DGELVGEYSAANLLIATGNGLFAFRRYQRNGEYYTLYLKSLDGLTVVASEPLDADPGW
                     RPLADGELVELSQGAPRSALLTLST"
     gene            complement(2510..>2706)
                     /locus_tag="VF357_03020"
     CDS             complement(2510..>2706)
                     /locus_tag="VF357_03020"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7523147.1"
                     /translation="PSKLMTYFPELGRLTEKVIFGSDWPGMPWIRRNMEAIGKLPLPA
                     EGVERILGGNAARLLRIQGT"
BASE COUNT          518 a          859 c          926 g          403 t
ORIGIN      
        1 gggcgaggga cgcaatcgtt cggaaattgt tcacgtgatt gtactccctg tcgtcacgga
       61 ttcaacccga cgggagtgga aacgtccccc ggagcggccg gcgtccgctc aatctccaca
      121 atagtgacct ccggaggggc gaggaaccgc atcgggggcc cccacgttcc cgtgcctcgg
      181 ctcacgtgca ggatcccgcc ccccgggacc aggtggtccc ccccgagcag ggggaaggcc
      241 aggcgggtga ggaggcggaa cggaaagatc tgcccatcgt gggtatgacc cgagagctgc
      301 agatcgaatt tccctcccgt ggccgggtcc agttgagggc ggtgcttgag gaggagtgtg
      361 aaccgtccgt ccgggcgatt tcccaaaagg gcggcttcgt cggtcccgcc ggttcggccg
      421 aaccgtgcgg cggcgggatc gtcgactgct gcgatgcgca ccgcgtcgtc gatcgtcacg
      481 gattcgtccc gaaggaccgt gaagccggac ttccgggtga aggcgatcga acggtcgatc
      541 cccgcgtaat attcgtggtt cccgagggcc gcgaatttcc cccgcggcgc ggggatcccg
      601 cgcaggatct cctccagctc cgcgacgccg tcgagctgtc cgtccacgag gtctcccgtc
      661 gatacgaaga tgtccgggtg ctcccgggcg acgatcgccg cgacctccct ggccttgccg
      721 ttccggtgga tgagaccgag gtggaggtcg gagatctgcg cgatccgaag ggaggggacg
      781 gacgccggga gtcggtccgt gacgatgcgc acacggacga cctggatgcg ggaggcttcg
      841 acgatggcgt acgccgaaag cgcgacggcg aggccggcga cgcagagaaa cgcggggcga
      901 cccgcgagcc cccccgccgt ggtcgcggcg atcccgccgc gccccatcgc caacagagga
      961 aggcggagga ggtcgacgga aaggttcagg caggtgaaaa agaagagaaa ccccatccag
     1021 aggtagccga cgtacgcgac gatccgtgcc gcctcttcgt agccgtggcg gctcaacgaa
     1081 aaggtcagga tcggcgcgca caggagaatt ccgaggagga ggagaagcga gagcgtcgtc
     1141 ccgggagcga aggccagcgc agaccgcgcc ttgagaaggg cgtaggcgtg catggaaccg
     1201 tagatcagga agaacgaaag caggaacagg ctcatggcga atgtgcgata atcagcgggt
     1261 acgaggaccg gtcctgacgg aggagaacgt gcccacctac gagtacaaat gccccgcgtg
     1321 cggggagttc gagaaggagc agcggatgtc cgccccgccg ctcaagaagt gcccccattg
     1381 cggcaaggcg gtgacccggc tggtcggcgg cggcggcggc ttcgtgctga agggcggaaa
     1441 ctgggtctcc aagatgtcct cttcggggga atccgccaag aagaaatccg accggttcat
     1501 gaaacagacc gtgggcgagg tcgccgaaga tatcgcgaag cactcccggt cgcattaggt
     1561 ggacagggtg agcagggcgg agcggggggc gccctgcgaa agttcgacca gttccccgtc
     1621 cgcgagggga cgccacccgg ggtcggcgtc cagcggctcg ctcgcgacga ccgtgagccc
     1681 gtccagggat ttcaggtaca gggtgtaata ttccccgttc ctttggtacc gacggaacgc
     1741 gaaaagcccg ttgccggtgg cgatcagcag gttcgccgcg gaatattccc ccaccagttc
     1801 accgtcggag aggatccctc gaagcgcttc ggataacccc tccagcgtcc gctccaccca
     1861 ccggtcctcg aggagttcga ggaagaccac ggtatcgctg accttgcggg catccccttc
     1921 cgcgccgatc ttcccgtaaa aggtcccgtt gtgggcgagg gtcactccca gcgcctggaa
     1981 cgggtgcgag ttcgccgcgc atacggtttc cggattcgac gcgtatcgga catggccgag
     2041 gaaccggtcg gtcactgcgc gcagaccgaa aagtagcggg tcggacgcgg cgggactccc
     2101 gcttttcacc atccggatct cctcccccgc gcgccaggcg accccccagc cgttcggatg
     2161 gttccctccc ggccgcctct cccagcctgc gacgaggttc cccgattcgc aaaaccgcgc
     2221 aagggaggcg aggtacggcg cgacgtcgcg gggctcgttc gacgaaaacg cgatcatccg
     2281 gcacatgatt acatgatacc cgcttccagc acaccgcaac accccggagg cgcatcgggg
     2341 ggggtccgcg cagcggcgtc tggagcgcag cggagacaag gacgtcggga gcaagcggaa
     2401 gcgcaggcga ggagaccatg gacggtcgac gagccgtagc ggagaggaag ccccccgcga
     2461 gcccagagcc ccctgcgtca gaatgttatc gtatttataa tacgcagcgc tacgtgccct
     2521 gaatacgcag cagccgggcg gcgttgccgc cgaggatgcg ttccacccct tccgccggca
     2581 acggcaactt cccgatcgct tccatgttgc gccggatcca cggcatcccc ggccagtcgc
     2641 tgccgaagat caccttttcc gtgagccggc ccagttcagg aaaatacgtc atcagcttcg
     2701 acgggg
//
LOCUS       DASVJL010000153         7330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_23772, whole genome shotgun sequence.
ACCESSION   DASVJL010000153 DASVJL010000000
VERSION     DASVJL010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23772"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VF357_03025"
     CDS             <1..1136
                     /locus_tag="VF357_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HEX7523148.1"
                     /translation="EQSNSSVTFGDKMILKVFRRVDPGINPELEIGAYLTEVAAFPHV
                     PPVAGSFQYARAHEEPMTLAILMGFVPNQGDAWRYTLDSLGQFFERVLTQSADIAELP
                     CTGPRAVRAGEESVPPKVSELIGSYLLSARQLGERTAEFHLVMASNVGNPSFAPEPFT
                     PFYQRSLYQSMRNLTARTFSLLRKRVRTLPETLREHGREVLDMEARILKRFQGILDRK
                     ITAMRIRIHGDYHLGQVLHAGKEFVLIDFEGEPARSLADRRIKRSPVRDVAGMLRSFH
                     YASYAPLIGDGGGGVRSGDIKSLEPWADFWNRWVSSAFLQGYLAVAREGEFLPRTEED
                     LSFLIDTFLLEKAIYELGYELNNRPDWIKLPLLGILSQFEAPE"
     gene            1133..3283
                     /gene="malQ"
                     /locus_tag="VF357_03030"
     CDS             1133..3283
                     /gene="malQ"
                     /locus_tag="VF357_03030"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX7523149.1"
                     /translation="MTSGSDREIRELRRLARVRGVQTSYFDIRHQRRAPPPETLLAVL
                     RSMGEPLETVRDAPEALGRAESSAWGRVCPPVCVAWDHAPALVELRVPRGAENARVRL
                     LVVLETGEARVFDCDLSLFPVSATGTGPAGRCVAKRIPIPWRLPPGYHTLRVELGKAN
                     SVTTVVSAPPRAWPGPDGNGRRWGVFLPLYALHSRRSLASGDLTDLRSLMDWVEGMGG
                     DAVGTLPLLSTCSPEPFNPSPYSPCSRLAWNEFYLDVTRAPGFGECRAARELLASAEF
                     QAETGRLREAPLVDYRRGTALKRRVLEELAEAFCRKDPAAQGDFRRFVAENRPIAEYA
                     RFRAAWDTIGSPWPTWPSPLREGPLGAGDFDGAAERYHLYVQWALHLQLRDLSSDSRK
                     LLYLDLPLGASYDGYDVWRRRDLFALQASAGAPPDDFFTKGQDWGFPPLHPERIREDG
                     HAYFRACIANQLRYAGILRIDHVMGLHRFFWIPNGAGPREGTYVRYPAEELYAILCLE
                     SHRHRARIVGEDLGTVPPYVRPAMARHGVRRMFVLQYGLMPDSGKALGKIPETSLACV
                     NTHDMPPFAAFLDGLDIEDRVSLGLLDGEEATREKTGRKERTRALTEFFRKRGQLPRT
                     GEDPDGETLLRACLAHLAGSSAEMVIVGLEDLWLERSPQNTPGTSSERPNWVRKSRHP
                     LEEFRDHPAVIRALREIDRIRKGGDPRCDTTWPC"
     gene            3259..5172
                     /gene="glgB"
                     /locus_tag="VF357_03035"
     CDS             3259..5172
                     /gene="glgB"
                     /locus_tag="VF357_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX7523150.1"
                     /translation="MRYDVALLTDQDLYLFNEGNHFRLYEKLGAHSLGSGSDAGTCFA
                     VWAPNAEEVSVMGDFNGWDNRANPLRRRGDSGIWEGFLPGGNVGSAYKYHVISRFGGY
                     RYDKSDPFAFFSQAPPEPGSIVWDLRNEWGDAEWMATRRRRNAADRPMAIYEVHLGSW
                     MRAHEEGNRPLTYRETAPRLASYLTRMNFTHVEFLPVMEHPFYGSWGYQGTGYFAPTS
                     RYGTPQDFMYLVDTLHRHGIGVLLDWVPSHFSKDGHGLNFFDGSHLYEHADPRQGHHP
                     DWDTEIFNYGRKEVQSFLLSSAFFWLDVYHADGLRVDAVASMLYLNYSRKDGEWIPNR
                     YGGRENVDAIAFLRRFNETVYEAFPDIVTIAEESTAWPMVSRPNYVGGLGFGMKWDMG
                     WMHDMLVYMSQDPVYRKFHHNKLTFRGMYAFSENFVLPLSHDEVVHGKGSLLGKMPGD
                     EWRKFANLRTLLGYMYAQPGKKLLFMGGEFGQLREWNHDTSLDWHLAEEPRHAALSRW
                     VEELNRFYRDEPALHELDFDPQGFEWADTRDAEQSVVSLFRKTRGGDDILLAVFNFTP
                     VPRHNYRIGVPRGGFWRECMNSDAKEYGGSGQGNLGGVEAAPIGAQGRLHSLTLVVPP
                     LSALFLKSEGPKA"
     gene            5169..7031
                     /gene="treZ"
                     /locus_tag="VF357_03040"
     CDS             5169..7031
                     /gene="treZ"
                     /locus_tag="VF357_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX7523151.1"
                     /translation="MTDPRTHPLGATPLPGGRCRFLVFAPSARAVEVHLLSPAERVAG
                     LERDVSGYHHAVVDGVNAGALFLYRLDHGVERPDPASRHQPQGVHGPSMVTESGAFAW
                     RDREWRGIPLSSYILYELHVGTYTPEGTFDAVIPRLDSLVELGITAVELMPVAQFPGK
                     RNWGYDGVFPFAVQESYGGPEGLKRLVDACHLRGLAVVLDVVYNHLGPEGNYLSDFGP
                     YFSDRYRTPWGSAVNFDGPFSDEVRRYFQENALCWLREFHVDALRLDAIHGIMDCSAS
                     PFLSELAGAVRDLRREENRMAYLIPESDLNDARIVTPRDEGGYGLDAQWSDDFHHALH
                     TLLTGEREGYYGDFGGIGHMARAFTDGFVYSGQSSEYRRRRHGNPSRHLPAGKFVVFA
                     QNHDQVGNRMRGERLARLVCFESLKLAAGVVLLSPFLPLLFMGEEYGEVAPFLYFVHH
                     GDTDLIEAVRKGRKEEFVAFGWKGEIPDPQDEGTFLRSRPDPALRASGNHARLLELYR
                     ELIRIRKTNPVLSRTNREGMEVSSFEKENALVVLRRNGPALAAAVFHFGDAPATLPLS
                     LPVGRWGKVLDSSDARWGGPGGAAPERPDPRAGSVLAVRPRSFILLSIPNKENR"
     gene            7032..>7330
                     /locus_tag="VF357_03045"
     CDS             7032..>7330
                     /locus_tag="VF357_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523152.1"
                     /translation="MDRYVCIHCHFYQPPRENPWLEAIELQDSAYPFHDWNERISAEC
                     YAANAFSRAHTGDGRIEKIVNNYARISFNFGPTLLSWMEEKSPAVYRAILEGDQE"
BASE COUNT         1205 a         2458 c         2439 g         1228 t
ORIGIN      
        1 ccgagcagag caacagttcg gtgaccttcg gggacaagat gatcctcaag gttttccgcc
       61 gcgtcgaccc ggggatcaac ccggaactgg agatcggggc ctacctgacc gaagtggcgg
      121 ccttccccca tgtccctccc gtcgccggat ccttccagta tgcgagggcg cacgaggaac
      181 cgatgaccct tgcgatcctc atggggttcg tcccgaacca gggggacgcg tggcgataca
      241 ccctggacag cctcgggcag ttcttcgagc gggtcttgac gcagtccgcc gacatcgcgg
      301 aattgccttg cacgggacct cgcgcggtcc gggcgggcga ggaaagcgtt cctccgaaag
      361 taagcgaact catcggttcg tacctcctgt cggcccgcca gctgggagag cggacggcgg
      421 agttccacct tgtcatggcc tcgaacgtgg ggaatccgtc cttcgccccc gagccgttca
      481 cgccgttcta ccagcggtcc ctgtaccagt cgatgcggaa cctcaccgcc cggaccttct
      541 ccctgctgcg gaagcgggtc cggaccttgc cggaaaccct tcgcgagcac gggcgggagg
      601 tactggatat ggaagcgagg atcctgaagc ggttccaggg aatcctcgac cggaagatca
      661 cggcgatgcg gatccggatc cacggcgact accatctcgg acaggtgctc cacgccggaa
      721 aggaattcgt cctcatcgat ttcgaaggag agccggcccg ctccctggcc gaccgccgga
      781 tcaaacggtc gccggtgcgg gacgtggcgg ggatgctccg ttcgttccac tacgcctcct
      841 atgctcccct catcggggat ggcgggggcg gcgtccggtc gggcgacatc aaatccctcg
      901 agccgtgggc cgatttctgg aaccgttggg tgtcctcggc gttccttcag ggatacctcg
      961 ccgtcgccag ggaaggagag ttcctgcccc gtaccgagga ggacctgtcg ttcctgatcg
     1021 acacgtttct tctggaaaaa gccatctatg agctggggta cgagctcaac aaccggcccg
     1081 actggatcaa gctcccgctg ctggggatcc tttcccagtt cgaggcgccg gagtgacctc
     1141 cgggagcgac agggagatcc gcgaactgcg gcgcctggca cgcgttcgcg gggtacagac
     1201 ttcctacttc gacatccgtc accaacggag ggcgccgcct ccggaaacgc tcctcgccgt
     1261 gcttcgctcc atgggggagc cgctggaaac ggtccgggat gcgccggagg cgctgggccg
     1321 ggcggaatcg tccgcgtggg ggcgcgtctg cccgccggtc tgcgtcgcct gggaccacgc
     1381 gcccgccctg gtggagcttc gggtcccccg cggcgccgaa aacgcgcgcg tgcggctcct
     1441 cgtcgtcctg gaaaccgggg aggcgcgggt cttcgactgc gacctgtccc tttttcccgt
     1501 ttccgccacc gggaccgggc ccgcggggag gtgcgttgcg aaaagaatcc cgattccatg
     1561 gagactgccg ccgggatacc acacgctgcg ggtggagctg ggaaaggcga acagcgtgac
     1621 gacggtcgtt tccgccccgc cgcgcgcatg gcccggcccg gacgggaacg gccgaaggtg
     1681 gggggtgttc cttccgctgt acgcgctcca ttcccgcaga agcctcgcca gcggcgacct
     1741 cacggacctg cgctccctca tggactgggt ggaagggatg gggggcgacg cggtgggaac
     1801 gctgccgctg ctttcgacct gctcccccga gccgttcaac cccagtccct attccccctg
     1861 cagtcgtctc gcatggaacg agttctatct cgacgtgacc cgggcgcccg ggttcgggga
     1921 gtgccgcgcg gcaagggaac tgctcgcctc ggcggagttc caggcggaga cgggccggtt
     1981 gcgggaagcg cccctcgtcg attaccggcg cggcacggcg ctgaagcgcc gcgtgctcga
     2041 ggaacttgcg gaagctttct gccggaagga tcccgcggca cagggggatt tccggcgatt
     2101 cgtcgccgag aaccgtccga tcgcggagta cgcccgcttc cgtgcggcct gggacacgat
     2161 cgggtcccca tggccgacct ggccgtcgcc gctccgtgaa gggcctctgg gcgcggggga
     2221 tttcgacggg gcggccgaac ggtaccacct ttacgtgcaa tgggcgcttc acctgcagct
     2281 ccgggacctc tcgtccgact cccggaagct gctgtacctc gaccttcccc tcggagcgag
     2341 ctacgacggc tacgacgtgt ggcggcgtcg ggacctcttc gcgcttcagg cgtccgcggg
     2401 ggcgcccccc gacgatttct tcaccaaggg acaggactgg gggttccctc cgctgcaccc
     2461 cgagcggatc cgggaggacg ggcacgccta tttccgcgcg tgcatcgcga accagctgcg
     2521 ctatgcgggg atccttcgga tcgaccacgt catggggctg caccgcttct tctggattcc
     2581 gaacggcgca ggtccccgcg agggaaccta cgtgcggtat cccgcggagg agctgtacgc
     2641 catattgtgc ctggagtcgc accggcaccg ggcgcggatc gtgggggagg atctcgggac
     2701 ggtccccccg tacgtgcggc cggcgatggc acggcacggg gtgcgccgga tgttcgtcct
     2761 gcagtacggg ctgatgccgg actccggcaa ggccctgggg aagataccgg agacttcgct
     2821 tgcgtgcgta aacacccacg acatgccccc cttcgccgcc ttcctcgatg gcctcgacat
     2881 cgaagaccgc gtgtccctgg gcctcctgga cggagaggag gcaacccggg aaaaaaccgg
     2941 gcggaaggaa cgcacgcggg cgctgacgga gtttttccgg aaaagggggc aattgcctcg
     3001 gaccggagaa gaccccgacg gggaaacgtt gctccgggcc tgcctggccc accttgcggg
     3061 gagcagtgcg gaaatggtga tcgtcggcct ggaggacctg tggctggagc ggtcccccca
     3121 gaacaccccc gggacctctt cggagcgccc aaactgggtc cggaagagtc ggcatcccct
     3181 cgaggagttc cgcgaccatc cggcggtgat ccgcgcgctg cgggagatcg accgaatccg
     3241 aaagggaggt gacccccgtt gcgatacgac gtggccttgc tgaccgatca ggacctctat
     3301 ttattcaacg aggggaacca cttccgcctc tacgaaaagc tcggggccca ttccctcgga
     3361 tcgggttccg acgcgggcac ctgtttcgcc gtgtgggcgc ccaacgcgga ggaggtctcc
     3421 gtgatggggg atttcaacgg gtgggacaac cgcgcgaacc cgctgcggcg acggggggat
     3481 tcgggaatct gggaagggtt cctcccgggc gggaacgtcg ggtcggccta caagtaccat
     3541 gtgatttcgc gtttcggggg gtaccggtac gacaagtccg acccgttcgc gttcttctcc
     3601 caggctcccc cggagcccgg atccatcgtg tgggacctcc ggaacgaatg gggcgatgcc
     3661 gagtggatgg ccacccggcg ccggcggaac gccgcggacc gccccatggc catctacgag
     3721 gtgcaccttg gatcctggat gcgggcacac gaggagggga accgcccgct gacgtaccgg
     3781 gagaccgctc cccgcctcgc ctcctacctg acccggatga atttcaccca cgtggagttc
     3841 ctccccgtga tggaacaccc tttctacggc tcctggggat accaggggac ggggtacttc
     3901 gccccgacaa gcaggtacgg gaccccgcag gacttcatgt acctcgtgga caccctgcac
     3961 cggcacggga tcggcgtcct cctggactgg gtcccctccc acttctccaa ggacgggcat
     4021 gggctcaact tcttcgacgg ctcgcacctc tacgagcacg ccgatccccg ccaggggcac
     4081 cacccggact gggacacgga gatcttcaac tacgggcgca aggaggtgca gagctttctc
     4141 ctcagcagcg ccttcttctg gctggacgtg taccacgcgg acggcctgcg cgtggacgcc
     4201 gtcgcgtcga tgctctacct gaactactcc cggaaggacg gggaatggat ccccaatcgg
     4261 tacggcggcc gggagaacgt cgatgcgatc gctttcctgc ggcgcttcaa cgagacggtg
     4321 tacgaggcgt ttcccgacat cgtgacgatc gccgaggagt ccaccgcctg gcccatggtt
     4381 tcccgtccga actacgtggg gggcctgggc ttcgggatga agtgggacat gggatggatg
     4441 cacgacatgc tggtgtacat gtcccaggac ccggtctacc ggaaattcca ccacaacaag
     4501 ctcacgttcc ggggaatgta cgccttctcg gagaacttcg tgctccccct ttcccacgac
     4561 gaggtcgtgc acgggaaggg gtcgctcctc gggaagatgc ccggcgacga atggaggaag
     4621 ttcgcgaacc ttcgcacctt gctcgggtac atgtacgccc agccggggaa gaagctcctc
     4681 ttcatgggag gggagttcgg gcaattgcgg gagtggaacc acgacacgag cctggactgg
     4741 cacctcgcgg aagaaccgcg gcacgccgcc ctctcccgct gggtggagga gctgaaccgg
     4801 ttctaccggg acgagcccgc cctgcacgaa ctcgatttcg atccgcaagg gttcgagtgg
     4861 gccgacaccc gggacgcgga acagagcgtg gtcagccttt tccggaagac ccgcggaggg
     4921 gacgatatcc tcctggccgt cttcaatttc acccccgtcc cgaggcataa ttaccggatc
     4981 ggcgttcccc gcggcgggtt ctggcgggag tgcatgaaca gcgacgcgaa ggagtacggc
     5041 ggaagcgggc aggggaacct cggcggcgtg gaggcggccc ccatcggcgc ccagggccgg
     5101 ctccactccc tgaccctggt ggtcccgccg ctgtccgccc tgttcctcaa aagcgaagga
     5161 cccaaggcat gaccgaccct cgcacccacc ccctcggggc gacacccctc ccgggcggcc
     5221 gctgccgctt cctcgtgttc gcgccgtcgg cgcgggcggt cgaggttcac ctgctctccc
     5281 cggcggagcg cgtcgccggc ctcgagcgcg acgtgtccgg gtaccaccat gcggtcgtcg
     5341 acggggtgaa tgccggcgcg ctcttcctct accggctgga ccacggggtc gagcgccccg
     5401 accccgcctc gcgccaccag cctcaaggcg tgcacggccc ctccatggtc accgaatcgg
     5461 gagcgttcgc gtggcgggac cgcgagtggc gcgggatccc gctctcttcg tacatactgt
     5521 acgagctgca cgtcggaacg tacacgcccg aaggcacttt cgacgccgtc atcccgcgcc
     5581 tggactccct cgtggagctg gggatcaccg ccgtggagct gatgcccgtg gcgcagttcc
     5641 ccgggaagag aaactgggga tacgacggcg tcttcccgtt cgcggtgcag gagagctacg
     5701 gggggccgga agggctgaag cgcctggtcg acgcatgcca cctgcgcggc ctggccgtcg
     5761 ttttggacgt ggtctacaac catctcgggc cggaggggaa ttacctctcc gacttcggcc
     5821 cctacttcag cgaccggtac cgcacgccgt ggggatccgc cgtgaacttc gacggcccct
     5881 tcagcgacga ggtccggcgg tatttccagg agaacgccct ctgctggctg cgggagttcc
     5941 acgtcgacgc gctgcgcctc gacgcgatcc acggcatcat ggactgcagc gcctcccctt
     6001 tcctctccga gctcgccggg gccgtgaggg accttcggag ggaagagaac cggatggcct
     6061 acctgattcc ggagagcgac ctgaacgatg cccggatcgt cacgccccgg gacgaaggag
     6121 ggtacggcct cgacgcgcaa tggagcgacg acttccacca cgcgctccac acgctcctga
     6181 cgggggaacg ggaagggtac tacggcgact tcggcggcat cgggcacatg gcgcgcgcgt
     6241 tcaccgacgg gttcgtctac tcggggcaat cttcggagta ccgccggcgc cggcacggga
     6301 acccgtcgcg ccacctcccg gcggggaagt tcgtcgtgtt cgcccagaat cacgaccagg
     6361 tggggaaccg gatgcgcggg gagcggctcg cccgcctggt ctgcttcgag tccctgaagc
     6421 tcgccgcggg cgtcgtcctg ctttccccct tcctgcccct cctgttcatg ggggaggaat
     6481 acggcgaggt tgccccgttc ctctacttcg tgcaccatgg cgatacggac ctcatcgagg
     6541 cggtccggaa agggcggaag gaggagttcg tcgccttcgg ctggaaaggc gagatccccg
     6601 atccccagga cgagggtacg ttcctgcgtt cccgtccgga ccctgcgctc cgggcatcgg
     6661 gaaaccatgc gcgactcctg gagctttacc gcgagctgat ccggatccgg aagaccaatc
     6721 cggtcctgtc ccggacgaac cgggaaggca tggaggtgtc ctccttcgaa aaggaaaacg
     6781 cccttgtcgt cctgcgccgc aacgggccgg cgctggccgc ggcggtgttc catttcggag
     6841 acgcgccggc gacccttccc ctgtcccttc ccgtcggccg atggggaaag gttctggatt
     6901 cctccgacgc ccgttggggc ggtcccgggg gagcggcccc cgaacggccg gacccgcgcg
     6961 cgggatccgt tctcgcggtc cgcccgaggt cgttcatcct cctctctatc ccaaacaagg
     7021 agaaccgata gatggaccgt tacgtctgca tccactgcca cttctaccag ccgccgcgcg
     7081 aaaacccctg gctggaggcg atcgaactgc aggactccgc atatcctttc cacgactgga
     7141 acgagcggat ctccgcggaa tgttacgcgg ccaacgcttt ttccagggcc cacaccgggg
     7201 acgggcggat cgagaagatc gtcaacaact acgcccgcat cagcttcaac ttcggtccga
     7261 cgctgctgtc ctggatggag gagaaatccc ccgcggtgta ccgcgccatc ctcgaagggg
     7321 accaggaaag
//
LOCUS       DASVJL010000154         4060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_119086, whole genome shotgun sequence.
ACCESSION   DASVJL010000154 DASVJL010000000
VERSION     DASVJL010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119086"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VF357_03050"
     CDS             <1..739
                     /locus_tag="VF357_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7523153.1"
                     /translation="VEPSRGQVPKEEYELARTGAGFGEVYVSPLHVAMIMSAIASGGA
                     MPRPVLIDRIENRDGKPLYESSPMKWRDTVLPETANAVVRMMVKTVEIGTSHRTFGTP
                     ERTPLLYDMDVAAKTGSLSGWTPSVHFEWFAGVAPVGSPRLALAALVVNDNRWKIKGS
                     YVGKEAFNSYFGYPSTMPPVYAKARATRKWTRPARTAKKGEPTVKAKSKKGGKAGRVR
                     RRPPAKTAEKSVGKPLAVNVSTARTGG"
     gene            753..1739
                     /locus_tag="VF357_03055"
     CDS             753..1739
                     /locus_tag="VF357_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7523154.1"
                     /translation="MEFHRVIGARRSLRAFSQRPVETEKIERMLEAARWSPSCANRQP
                     WRFVVVGADAPSRAAVEEALDAGNAWAKRAPVLIAAGARREDAAVVESRDYFLLDTGM
                     ALMSLLYRAVDQGLLAHPMAGWKEEPLRAALGLPEDFTPAAVVAVGYAGKSGVLDEAA
                     RKKDEKPPARKPLGEVAFRSRWGEPFEATLPSLPGKVYETELQLRFGDTDAMGHVNNA
                     KVLTYLELGRMQFFADVMGVERVEDIRFILAEVSCRYRIPILLHDRVFVRMHITDVNR
                     SSFRFRCDLYDPRDGRVFVEAETVQVMYDYATGRPAPVDADFLDKARDYIGG"
     gene            1740..2663
                     /gene="mtgA"
                     /locus_tag="VF357_03060"
     CDS             1740..2663
                     /gene="mtgA"
                     /locus_tag="VF357_03060"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX7523155.1"
                     /translation="MPRAPRLKMRSASRFRKILAALAVIALVLAAWVVVSILTLPSVA
                     PLAKPGVSLTITVKDWHRKDHLFVVGPRNPRWTPYGAIPAALKKAVVASEDAHFYSHD
                     GVDYDAIQEAIKADWRKGKFVHGGSTITQQLAKNLFLTREKTLIRKVKELVLARRLDD
                     TLSKSRILELYLNVVELGPMVYGIGHAAHYYFGKPPSALTVRECAFLASMLPGPRVYN
                     PYRKMDRVMKRSDRILRRMVASRMISREEYDAAMAEVPNLAGLARKVEKTLETPPPEE
                     KPPEDPPGGGPAIIEGSPTAPPEPGSEESVR"
     gene            complement(2656..3105)
                     /locus_tag="VF357_03065"
     CDS             complement(2656..3105)
                     /locus_tag="VF357_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7523156.1"
                     /translation="MRLSKKTEYALRALIYAAGFPEGTSFQIRDLAEKNGIPKKFLEL
                     ILLELKNAGMLSSRRGVGGGYLLARRPDSIRSSEIVEVFEGPMAARGRQKGSKTEKED
                     SSAAVSRLVEEASAAAAAVFSRSTLADLVREEDDATQRRRRNVMYFI"
     gene            complement(3274..>4060)
                     /locus_tag="VF357_03070"
     CDS             complement(3274..>4060)
                     /locus_tag="VF357_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7523157.1"
                     /translation="HPAGLTILLAALVSAFLARKVLCSWVCPVGGISRALEWVGKKTI
                     WKRRKEETLLPMWADRALSAIKYLLLAFFLYAVVLRMDATAIMKFQRGTYNYAADAKM
                     LLFFTEMTGVTAVTLAVLALLSIVVKNFWCRYLCPYGALLGLVSWISPHRVVRDGQMC
                     IDCKACTRTCPVEIRVHEKPSVWTPECTGCMACVAACPVEDCLTVTRRGKAGWSPYLI
                     PLVGLGTIFLFWGIARVAGYWHSYVPDAQLAEAYRQAKGLLHP"
BASE COUNT          722 a         1310 c         1400 g          628 t
ORIGIN      
        1 ggtggagccg agccgtggac aggtgccgaa ggaagaatac gaactcgccc gcaccggcgc
       61 agggttcggc gaggtgtacg tctccccgct ccacgtggcg atgatcatgt ccgcgatcgc
      121 ttcgggcggc gccatgcccc gccccgtgct gatcgaccgg atcgagaacc gcgacgggaa
      181 gccgctgtac gagtcttccc cgatgaaatg gcgggacacg gtcctcccag agacggcgaa
      241 cgccgtcgtc cggatgatgg tgaaaacggt cgagatcggg acgtcgcacc ggacgttcgg
      301 tacccccgag cggaccccgc tcctgtatga catggacgtt gcggcgaaga ccgggtcgct
      361 ctccgggtgg acgccgagcg tccacttcga atggttcgcc ggggttgcgc ccgtcggatc
      421 gccgcggctg gcgctggcgg ccctcgtggt caacgacaac cggtggaaga tcaagggaag
      481 ctacgtcggg aaggaggcgt tcaactccta cttcgggtac ccatcgacga tgccgcccgt
      541 ttacgcgaag gcgcgtgcga cccggaagtg gacccggccg gcccgcacgg cgaagaaggg
      601 ggagccgacg gtcaaggcga agtcgaagaa gggcgggaaa gcggggaggg tccggaggcg
      661 cccccccgcg aagaccgcgg agaagagcgt agggaagcct ctggccgtga acgtttccac
      721 cgcaaggacc ggcgggtagc gctcccggat cgatggaatt ccaccgcgtc atcggcgcgc
      781 ggcgcagcct gcgcgcgttc tcccaacgtc ccgtcgagac ggaaaagatc gagcggatgc
      841 tcgaagcggc gcgttggtcc ccctcgtgcg ccaaccgcca gccgtggcgg ttcgtcgtgg
      901 tgggggcgga cgcgccgtcc cgcgcggcgg tcgaggaggc cctcgacgcg ggcaacgcct
      961 gggcgaagcg cgctccggtg ctgatcgccg cgggagcgcg gcgggaggac gccgccgtcg
     1021 tcgagtccag ggactacttt ctcctcgaca cgggaatggc gctcatgtcc ctcctctatc
     1081 gcgcggtcga ccaggggttg ctggcccacc cgatggcggg atggaaggag gagccgctac
     1141 gggccgccct cgggcttccg gaggatttca ccccggcggc cgtcgtcgcc gtcggctacg
     1201 ccgggaagag cggggtcctc gacgaggcgg cgcggaagaa ggacgagaag cccccggcgc
     1261 gcaagccgct cggggaggtc gccttccgtt cgcggtgggg ggagccgttc gaggcgacgc
     1321 tgccttccct gcccgggaag gtgtacgaga ccgagttgca gcttcgcttc ggcgacaccg
     1381 acgcgatggg gcacgtgaac aacgcgaagg tcctcaccta tctcgagctg ggccgcatgc
     1441 aattctttgc ggacgtgatg ggagtggagc gggtggagga catccggttc atcctcgccg
     1501 aggtttcctg ccgctaccgg atccccatcc tgcttcacga tcgcgtcttc gtgcggatgc
     1561 acatcaccga cgtcaaccgg agctccttcc ggttccgctg cgacctgtac gatccccggg
     1621 acggccgcgt tttcgtcgag gcggagacgg ttcaggtgat gtacgactac gcgacggggc
     1681 gccctgcccc cgtggacgcc gacttcctcg acaaggcccg ggactacatc ggaggatagg
     1741 tgccccgcgc gccgcgcctg aagatgcgat ccgcgagccg tttccgcaag atccttgccg
     1801 cgctcgccgt tattgcgctc gtcctcgcgg cgtgggtcgt cgtttcgatc ctcacgctcc
     1861 cctcggtcgc gcccctggcg aagccgggcg tttcgttgac gatcaccgtg aaagactggc
     1921 accggaagga tcacctcttc gtggtcgggc cgaggaatcc gcgctggacg ccgtacgggg
     1981 cgatccccgc cgcgctgaag aaggccgtcg tcgcctcgga ggacgcccac ttctactcgc
     2041 acgatggggt ggactacgat gcgatccagg aggcgatcaa ggccgactgg cggaagggga
     2101 agttcgtcca cggcgggagc acgatcacac agcagctggc gaagaacctc ttcctgaccc
     2161 gggagaaaac gttgatccgg aaggtgaagg agctcgtcct tgcgcggcgc ctggatgaca
     2221 ccttatcgaa atcgcggatc ctcgaactgt acctcaacgt ggtggaactc gggccgatgg
     2281 tgtacgggat cggtcacgcg gcgcactact acttcgggaa gcctccctcc gcgctgaccg
     2341 tgcgggaatg cgcgttcctc gcctcgatgc tccccggccc gagagtctac aacccgtacc
     2401 ggaagatgga ccgggtgatg aagcgatccg accggatcct gcggcggatg gtcgcgtcgc
     2461 ggatgatctc gcgggaggag tacgacgcgg cgatggcgga ggtcccgaac ctcgcgggac
     2521 tcgcgcggaa ggtggagaag acgctggaga ccccgccgcc cgaggagaaa ccgcccgagg
     2581 atccccccgg cggaggtccg gcgatcatcg aaggatcccc cacggcgccg ccggaacccg
     2641 gttccgaaga atcggtcaga tgaaatacat cacgttgcgc cgccgccgct gcgtcgcgtc
     2701 gtcctcttcc cggacgaggt cggcgagggt cgatcgcgag aagaccgccg cggcggcggc
     2761 ggacgcctcc tccaccagcc gggaaacggc ggcggaggaa tcctccttct ccgtcttgga
     2821 gcctttctgc cggccccgcg ccgccatcgg cccctcgaac acttcgacga tctcggaaga
     2881 ccggatcgaa tcggggcgtc gcgcgaggag gtacccgccc ccgaccccgc gccggctgga
     2941 gagcatcccg gcgttcttca attcgagcag gatgagttcg aggaacttct tcgggatccc
     3001 gttcttctcc gcgagatccc ggatctggaa ggaggtccct tccgggaagc cggcggcgta
     3061 gatcagcgcg cgcaaagcgt attcggtttt cttggaaagt cgcatgtccc ctccgggctg
     3121 tacacccaca tatcctatca gaaagatagg atatgtggga ccgcctcgga tttcaagcgg
     3181 aaaggggcga aacgcaatgg catgtgcttc agttcataaa acgctaatac gcgacccttg
     3241 aagagaagga ctccggctcg cggggagctg gagtcaggga tgcagaagac ccttggcctg
     3301 gcggtacgcc tcggcgagtt gggcgtcggg aacgtagctg tgccagtacc cggcgacgcg
     3361 ggcaatgccc cagaacagga agatcgtgcc gaggccgacc agcgggatga gatacggcga
     3421 ccatcctgcc ttcccccggc gcgttaccgt gaggcaatct tccacggggc acgccgccac
     3481 gcacgccatg caccccgtgc actcgggagt ccacacggac ggcttctcat ggacgcggat
     3541 ctcgaccggg cacgtccggg tgcacgcctt gcaatcgatg cacatctgac cgtcccgaac
     3601 gacccgatgg ggggagatcc agctgaccag cccgagcaac gccccgtacg ggcagaggta
     3661 gcggcaccag aaattcttca cgacgatcga cagcagcgcg aggaccgcga gggtgaccgc
     3721 cgtgacgccg gtcatctccg tgaagaacag gagcatcttc gcgtcggcgg cgtagttgta
     3781 cgtcccgcgc tggaacttca tgatcgccgt cgcgtccatc ctcaggacga cggcgtagag
     3841 gaagaaggcg aggagaaggt atttgatcgc ggagagcgcc cggtccgccc acatcggcag
     3901 cagggtctcc tctttccggc gcttccagat cgtcttcttc ccgacccatt cgagcgcccg
     3961 ggagatgccg ccgaccgggc agacccacga acagaggacc ttgcgggcga ggaaggcgga
     4021 gaccagcgcg gcgaggagga tcgtgagccc cgcggggtgg
//
LOCUS       DASVJL010000155         4682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1150466, whole genome shotgun sequence.
ACCESSION   DASVJL010000155 DASVJL010000000
VERSION     DASVJL010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150466"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..404)
                     /locus_tag="VF357_03075"
     CDS             complement(111..404)
                     /locus_tag="VF357_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523158.1"
                     /translation="MKYQRNAPKGFEGMVRSALSLHPSTPDGSAKISLRFDPSGTVSG
                     VDIRSDVPELKTALSRVGWEAGPLPAGYQIPCSGLNVNVTVTGPNLSVGVEIL"
     gene            1134..2828
                     /locus_tag="VF357_03080"
     CDS             1134..2828
                     /locus_tag="VF357_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523159.1"
                     /translation="MKKRTIAVTVALLAFLMAGSAFAANTATDPLVPNAAYTITISKL
                     SSVGQLVDIETVGGTTDNNGLLSFTLTRIPTKDEANFIFLTIKNGSGTVVRRGMTPAP
                     PLDDNNATGLNYLSTTQADGMLLAASTNRTDDPIMAAYLLIILRSPNVSPADLANIAG
                     LGGDALTDPDVGFESFLLSNGVDNATLSKMKGCLIYNPDNSAKTLRNFTRSFFDAVAA
                     TDNDAASSAMQKAGGFMAEIFLDAGACAGIDAGQILAAHNAAGDGAQAGGHMSALSAG
                     LMGSINTAMSSFDRKIAIVRISTEYTNALNALNASGAQVTQYLASVTGLMNSSALIDQ
                     QFADYYMDHTAYLAAHPGVTDNDVRNSMNTAYSTGWTQFQTDIAADNASITAMKASFQ
                     SANPSLMLPGDFGTYTDQNGDRRNWPIPQVVLMNWLAASTFDYSARDNTAIPSMMGGW
                     MGACDNNMFWDQGSCVGSGHTWTPGRRDYVGSTGSSVFDSYLGLQEDLNIIQMKRNEI
                     WSTGHEPTGAERAANENLAGSRAQACGARITGSRSGDAITAAQKGAILKLLAPPEM"
     gene            complement(2905..4527)
                     /locus_tag="VF357_03085"
     CDS             complement(2905..4527)
                     /locus_tag="VF357_03085"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX7523160.1"
                     /translation="MPEFSYQDPFPLGKDTTKYRLLTKEHVSSSKFDGKEILKVDPEG
                     LTLLANQAMRDVSFLLRPSHNEKVAAILTDPESSANDRGVAVAMLRNAEVAANFILPF
                     CQDTGTATIVGKKGQQVWTGARDEEYLSKGVFKTYTEENLRYSQTIPLDLYEEINSGT
                     NLPAQIDLYATEGMEYKFLFVAKGGGSANKTYLFQETKALLNPASLEKFLVEKMKTLG
                     TAACPPYHLAFVVGGTSAEAALKTVKLASTGYLDHLPTKGNKGGQAFRDIEMEAKLLA
                     AARKSGYGAQFGGKHFAHDVRVVRMPRHGASCPVAMGVSCSADRNVKAKITKDGIWLE
                     ELDRNPGRLIPAKYRGKHEHGVVKIDLNRPMKEILAELSKYPVTTQLSLSGTIIVGRD
                     IAHAKLKERIDQGKGLPQYIKDHPIYYAGPAKTPKGKPSGSFGPTTAGRMDSYVDLFQ
                     SHGGSLVMIAKGNRSKAVTDACKKHGGFYLGSIGGPAALLAQENIKKVEVLEYPELGM
                     EAIWKIDVVDFPAFILVDDKGNDFYQQIACAG"
BASE COUNT          843 a         1469 c         1506 g          864 t
ORIGIN      
        1 ggtcgagacc gacaactggg ggatcggcgg agagaccgtt tcggcgcgcc ggaaatcggg
       61 acggtgaaaa cgggtcacat cccggatttc gtgcggggat cgctcccccg ttaaaggatt
      121 tcaaccccca cggacagatt cggtccggtg acggtgacgt tcacgttcaa cccggagcag
      181 gggatctgat acccggcggg gagtggtccc gcctcccatc ccacccggct gagcgccgtt
      241 ttcagttcgg gcacgtccga tcggatatcg acgccgctta cggttcctga aggatcgaac
      301 ctcaaggaaa tcttcgcgct cccatccggg gtcgacgggt gcagggaaag tgcggaccgc
      361 accatcccct cgaatccctt cggagcgttt cgttgatact tcaacgttct cgcgaagacc
      421 tcccggatcc aggtcatcct ggcgaaatcg acgttcagcc gcgttcccgg tgcggcggtt
      481 cccccgcggt cgccttccgg actcccaggt ttcggagaag gggcttcgga ggtccccgct
      541 tcctttatcg acgaggattc ctccggtacc gaggaggatt tttttggcga tgctgcgggc
      601 ggaggagcga aaaccggggc gggagaatca acacactcga ccatcatcac gcttcccgcg
      661 gcagggagcc tcccaccgtc gacaaaggtg tgcctggtcc acgatcccgc cagggtgaat
      721 aaccccatgt ggatgatcat ggagagcaga aggcttgtct ggaggcggcg gctcattccc
      781 ggttcttccc tgttcttcgt atcgggtgat cggggaggaa tctttaatcg tgtcggtccc
      841 gtaaaaaacc gttttgttca aggattcccc ttaagttttc gaagagatgg ccgataaggt
      901 taggggaggg gggaatcttg atcccgcctc cttggaggat tcgatgcgga aaatgttttt
      961 ctcgagtttg gcggtgctgg tgatcgtggc gggcatggcc ctgtcgggct gtggcggtgg
     1021 aggcagcagc agtagcagca gcggcagcgc tccctcacct tcttccccca gcgggacggt
     1081 tgaaggcaat gcgaaataat ctgcaaaaat cgatctacgg ggagaggaat caattgaaaa
     1141 agagaacgat cgccgttacg gttgccttgc ttgccttcct gatggcaggg agcgcattcg
     1201 ccgcgaatac cgccacagat ccactggtac cgaacgccgc gtacaccatc accatcagca
     1261 agctctcttc ggtcggacag ttggtggaca tcgagaccgt cggcgggacc accgacaaca
     1321 acgggcttct ttcgttcacg ttgacccgga tcccgaccaa ggacgaagcg aatttcatct
     1381 ttctcacgat caagaatggg agcggcacgg tcgtccgccg ggggatgacg ccggcgcctc
     1441 cgctcgacga caacaacgcc accgggctga actacttgtc cacgacccag gcggacggaa
     1501 tgctcctggc cgcgtcgacg aaccggaccg acgacccgat catggctgcg tacctgctga
     1561 tcatcctccg gtcgcccaac gtatcccctg cggacctggc caacatcgcc ggattggggg
     1621 gggacgcgct caccgatccg gacgtggggt tcgaaagctt cctgttgtcg aacggggtcg
     1681 acaacgcgac gctttccaaa atgaaagggt gcctgatcta caacccggac aacagtgcga
     1741 agacgctgcg gaacttcact cgtagcttct tcgacgcggt cgccgcgacc gacaacgacg
     1801 cggcctcgtc cgcgatgcag aaggcgggcg gcttcatggc cgagatcttc ctcgacgcgg
     1861 gcgcgtgcgc cggcatcgac gcggggcaga tcctcgccgc gcataacgcg gcgggggacg
     1921 gcgcgcaggc gggcggccac atgagcgccc tgtccgccgg cctcatgggc tccatcaaca
     1981 cggccatgag ctccttcgac cggaagatcg ccatcgtccg gatctcgacg gaatacacca
     2041 acgcgctgaa cgcgctgaac gcatcgggtg cgcaggtgac gcagtacctc gcctccgtta
     2101 ccgggctgat gaactcgtcc gccctgatcg atcagcagtt cgcggattac tacatggacc
     2161 acaccgccta tctggcggcg caccccgggg tgacggacaa cgacgtccgg aactccatga
     2221 acacggcgta cagcaccggg tggacgcagt tccagacgga catcgcggcc gacaacgcgt
     2281 cgatcaccgc gatgaaggct tcgttccagt cggcgaaccc gtcgctcatg ctgcccggcg
     2341 acttcggcac ctacacggac cagaacggag accggcggaa ctggcccatc ccgcaggtcg
     2401 tcctgatgaa ctggctggcg gcctcgacct tcgactacag cgcccgggac aacaccgcca
     2461 tcccgtcgat gatggggggg tggatgggag cgtgcgacaa caacatgttc tgggaccagg
     2521 gaagctgcgt gggcagcggc cacacgtgga cccccggccg gcgcgattac gtcgggtcca
     2581 ccggctcctc cgtgttcgat tcctacctgg ggctccagga ggacctgaac atcatccaga
     2641 tgaagcgcaa cgaaatctgg tcgaccggtc atgagcccac gggagcggag cgggcggcga
     2701 acgagaacct cgccggaagc agggcgcagg cctgcggggc gcggatcacc ggcagcaggt
     2761 cgggcgatgc gatcaccgcc gcgcaaaaag gtgcgatcct gaaactgctg gctccgccgg
     2821 agatgtgatc cggaacgaga aacgtgaaac gggtgggaga gcttcctctc ccacccgttt
     2881 cgtcttcggg agtatccccc tgcctcagcc ggcgcaggcg atctgctggt agaagtcgtt
     2941 ccccttgtcg tccaccagga tgaacgcggg gaagtccacg acatcgatct tccagatcgc
     3001 ctccatcccc agctcggggt attcgagcac ctcgaccttc ttgatgttct cctgcgcgag
     3061 cagcgccgcc ggaccgccga tcgaaccgag gtagaacccg ccgtgcttct tgcacgcgtc
     3121 ggtcaccgcc ttgctccggt tccccttcgc gatcatgacg agcgatccgc cgtgggactg
     3181 gaacaggtcc acgtacgagt ccatccgccc cgcggtggtg gggccgaacg agccggacgg
     3241 cttccccttc ggagtcttgg cggggccggc gtagtagatc gggtggtcct tgatgtattg
     3301 cggcagcccc ttcccctggt cgatccgctc cttcagtttc gcgtgggcga tgtcccgccc
     3361 gacgatgatc gtcccggaga gggagagctg cgtggtgacc gggtacttcg acagctccgc
     3421 gaggatctcc ttcatcggcc ggttgaggtc gatcttgacc accccgtgct cgtgcttccc
     3481 gcggtacttc gccgggatca ggcggcccgg gttccggtcg agctcctcga gccagatccc
     3541 gtccttggtg atcttcgcct tcacgttccg gtccgccgaa caggaaacgc ccatcgcgac
     3601 ggggcaggag gcgccgtggc gcggcatgcg gacgacccgc acgtcgtgcg cgaaatgttt
     3661 cccgccgaac tgcgccccgt agccggactt gcgcgcggcg gcgaggagct tcgcctccat
     3721 ctcgatgtcg cggaacgcct ggcccccctt gttccccttc gtcggaagat ggtcgaggta
     3781 gccggtggag gcgagcttga cggttttcag ggccgcctcg gccgacgtcc cgccgacgac
     3841 gaacgcgagg tggtacggcg ggcacgccgc ggtcccgagc gtcttcatct tctcgacgag
     3901 gaacttctcg agcgaggcgg ggttgaggag cgccttggtc tcctggaaaa ggtacgtctt
     3961 gttcgccgac ccgccgccct tggccacgaa gaggaacttg tactccatgc cttccgtggc
     4021 gtagaggtcg atctgggccg ggagattcgt ccccgagttg atctcctcgt acaggtcgag
     4081 ggggatcgtc tgggagtacc ggagattctc ctccgtgtac gtcttgaaga cgcctttgga
     4141 gaggtactcc tcgtcgcggg cgccggtcca cacctgctgc cccttcttcc cgacgatcgt
     4201 cgcggtcccc gtgtcctggc agaacgggag gatgaagttg gcggccacct cggcgttgcg
     4261 cagcatcgcg accgctaccc cgcggtcgtt ggcggacgac tcggggtcgg tcaggatcgc
     4321 ggcgaccttc tcgttgtggg aggggcgcag gaggaaggag acgtcccgca tcgcctggtt
     4381 cgccaggagc gtgagcccct ccgggtcgac cttgaggatc tccttcccgt cgaacttcga
     4441 cgaggaaacg tgctctttcg tgaggagtcg atacttggtg gtgtccttcc ccaaggggaa
     4501 cgggtcctgg tacgagaatt ctggcatcgg gcggctcctt ccgaagatgg gatgaggggg
     4561 gtgggaaaca tccagaagat agtcccgcgg ggacgaaacg tcaaggagaa gggactacgg
     4621 gggacgacgc cacccggaca attttctgcc gcggaatttc cgttcgggtc ttccttcggc
     4681 gg
//
LOCUS       DASVJL010000156        10509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_212035, whole genome shotgun sequence.
ACCESSION   DASVJL010000156 DASVJL010000000
VERSION     DASVJL010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 10509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10509
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212035"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..304
                     /gene="rpmF"
                     /locus_tag="VF357_03090"
     CDS             122..304
                     /gene="rpmF"
                     /locus_tag="VF357_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX7523161.1"
                     /translation="MPNPKRRGSKCRRDKRRTHKKLSQPAVSICPQCKATKRPHAVCP
                     TCGTYKGREVIAKSED"
     gene            321..1325
                     /gene="plsX"
                     /locus_tag="VF357_03095"
     CDS             321..1325
                     /gene="plsX"
                     /locus_tag="VF357_03095"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX7523162.1"
                     /translation="MKIAVDAMGGDHAPREIVRGAVESARSNGLSLILVGQEERIRAE
                     LREIDVSGADIQVLHASEVVEMCEVPGIALRKKRDSSIRVGLRLVADGKASSFVSAGN
                     SGAVMAGGFLILKKINGVDRPAIAATIPTPHGPVVLVDAGANVESKPAHLLQFGYMGE
                     AYSRTILGIPRPRVGVVSIGEEDSKGTDLTRDTCELFRRTGLNFVGNVEGRDFFAGKA
                     DVFVCDGFVGNVAIKTMEGMATALGQFLKEEIGKSLMAKVGAFLAERALRGVKDRLDY
                     EEYGGAPLLGVRGGVFICHGSSSERAIKNGIRAAGSLARCGVDVEIARSIAARGHAAG
                     "
     gene            1369..2346
                     /locus_tag="VF357_03100"
     CDS             1369..2346
                     /locus_tag="VF357_03100"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX7523163.1"
                     /translation="MASKIVGLGMYAPPKELTNLDLEKLVDTNDAWITERTGIRTRHI
                     AEPGTPNSDLCVEAARKALDDAGVDPAELDIVVIGTPTPDMPFPSTACFLQAKIGATR
                     AYGMDLSAACSGFIYSLSVTDALIRAGRGKKALVVGSEILSTVVDYTDRSTCILFGDG
                     AGAAVLTECPEGEGILSCHLHSDGNLWKLIYCPGGGTLHPYSPEMVEQRLRSIRMAGN
                     ETFKHAVLRMVEVSREALDRSGVGIDDVKLFIPHQANLRIIQVVGKRLGIPEERVFVN
                     LERYGNTSAASIPIALAEAKEQGRFAAGDLVLVVAFGGGLTWASALMRM"
     gene            2355..3287
                     /gene="fabD"
                     /locus_tag="VF357_03105"
     CDS             2355..3287
                     /gene="fabD"
                     /locus_tag="VF357_03105"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX7523164.1"
                     /translation="MGIGLLFPGQASQFPGMGKDLHDAYPVARRVFEEASEALSRDMA
                     ALCFLGTEEELRMTENTQPAIFIVSVAAFRVLAAETGVRPACAAGHSLGEYSALVAAG
                     ALPLREAVRVLRSRGKYMQEAVPVGEGAMAAILGLSPSQVEEACRAGAALGVVSPANF
                     NGGGQIVISGEAKAVAAACEAAKAAGAKRALPLAVSAPFHCALMRPAADRLAPELRAI
                     PQGPFAFPVVANVTAAPYGAGDVVADMLIRQITAPVRWEESVIAMRAAGADAFLEVGP
                     GKVLSGLLRRIGKDAATAVFCGPADLDGARGLPA"
     gene            3353..4096
                     /gene="fabG"
                     /locus_tag="VF357_03110"
     CDS             3353..4096
                     /gene="fabG"
                     /locus_tag="VF357_03110"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX7523165.1"
                     /translation="MRLSGKTALVTGASRGIGRAIAVRFAAEGAFVVVNYAGNEAAAG
                     ETLAAIASAGGNAVLSRFDVGDAAQVDAAVKAIVAERGRIDILVNNAGVTRDNLLMRL
                     TEDDFDAVVRTNLKGTFLVTKVVSRQMLRQRCGRIINMSSVVGEMGNAGQSVYAATKA
                     GILGFTKAMARELASREITVNAISPGFITTDMTEKLPEAARKEFSERIPLGRFGAPEE
                     VAELALFLASDAAAYVTGQVVGINGGMYM"
     gene            4142..4375
                     /gene="acpP"
                     /locus_tag="VF357_03115"
     CDS             4142..4375
                     /gene="acpP"
                     /locus_tag="VF357_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7523166.1"
                     /translation="MQVEQRVREIVAEQLERDVNEVTNTASFIDDLGADSLDIVELVM
                     KMEEEFGIEIPDEEAEKIKTVNDVIQYITIHKK"
     gene            4427..5665
                     /gene="fabF"
                     /locus_tag="VF357_03120"
     CDS             4427..5665
                     /gene="fabF"
                     /locus_tag="VF357_03120"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX7523167.1"
                     /translation="MRRVVVTGLGAVTPLGVGVRSTWDAILAGTSGVGPITRFDAKDF
                     STTIAAEVKGFDAEQFIDRKEIKRMDPFIHYAMAAAHMAMEDARLVIDDALAPKVGVY
                     MGSGLGGLSTLERYHKAYMEGGPRKISPFFITMLISNLAPGHIAMRYGAKGPNIATTT
                     ACAASSHATGEGMHAIRSGVCDVVIAGGAEATITPLGLGGFCSMKALSTRNDDPGTAS
                     RPFDKDRDGFIMGEGSAILILEELEHARSRGAKIYAELLGYGASADAYHVTAPAPGGE
                     GAVRAMTAALADAGVPASSIDYINAHGTSTSYNDLYETMAIKTVFGERAKSIAVSSTK
                     SMTGHLLGAAGAIEGMFCSLALQEGVIPPTMNYTTPDPECDLDYVPNALRRQAIRYAM
                     SNSFGFGGTNSVLLFGRFEA"
     gene            5670..7427
                     /gene="rpiB"
                     /locus_tag="VF357_03125"
     CDS             5670..7427
                     /gene="rpiB"
                     /locus_tag="VF357_03125"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX7523168.1"
                     /translation="MVPLTRRVVIASDHAGVEMKRRLREAMAALGISAEDLGTGSVGS
                     VDYPDYAAAVARRVSTGAADVGVLVCGTGIGMSITANKFPGVRAALLYDATAARFSRL
                     HNDANVAVFGARTMSADDAARRLRLFLSEPFEAGRHTRRLEKIRGIEKTIPAGPAPAA
                     NREAPMSFLKETDPEIYDIIRKETERQAYKLELIASENFVSEAVLEATGSVLTNKYAE
                     GYPGKRYYGGCEFVDQAESLAIERAKKIFGAAHVNVQPHAGSQANMAVYFAVMSPGDT
                     MLGMNLSHGGHLTHGSPVNFSGKLYNVVPYGVRGDTETIDYDQVRDLALKHRPKLIVV
                     GASAYPRTIDFPAFRRIADEAGCMVMADIAHIAGMVAVGLHPSPVPYCEFVTTTTHKT
                     PRGPRSGLIMCREEFAKKLDSAIFPGSQGGPLMHVIAAKAVALKEAMTPAFKEYQAQL
                     LRNAAAMAKILLARGYRLVSGGTDTHLILVNLKDTPLTGKEGEGALEHVGITVNKNTV
                     PFETRSPFITSGIRIGTPAVTTRGMKEKEMERIGNLIADVLAAPSDASVQRRVEAEVR
                     ALCDAFPLYASRLGAYAKG"
     gene            7433..7903
                     /locus_tag="VF357_03130"
     CDS             7433..7903
                     /locus_tag="VF357_03130"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEX7523169.1"
                     /translation="MPVTARTRPDWDTYFMDMAKLAARRSSCLRRAVGAVLVKDRRLL
                     ATGYNGVPSGVTHCEVVGCLRERLKVPSGERHELCRGLHAEQNAIIQAAYHGVSIRGA
                     QLYCTNLPCIICAKMLINAGVRRIIYLEGYSDTLTREMLDEVGMELLKLTDPSP"
     gene            7900..8376
                     /gene="nrdR"
                     /locus_tag="VF357_03135"
     CDS             7900..8376
                     /gene="nrdR"
                     /locus_tag="VF357_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX7523170.1"
                     /translation="MKCPRCGHVDNKVVDSRAGKDGDVIRRRRECLSCARRFTTYERI
                     EEELPLVVKRDGRREPYDRQKILSGIRKACEKRPVSYDTIEHLVEALEQEFQSGAEKE
                     ISTIRIGERVMSKLLQVDDVAYVRFASVYRQFKDVSQFVEEIKTLISEPPGRTTKS"
     gene            8373..9473
                     /gene="ribD"
                     /locus_tag="VF357_03140"
     CDS             8373..9473
                     /gene="ribD"
                     /locus_tag="VF357_03140"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX7523171.1"
                     /translation="MTARPEFPGTGFMRQALRLARKGAGRTAPNPAVGAVVVRGGRVV
                     GKGYHHAAGRPHAEIEALRRAGSAAGGADLYVTLEPCDHRGRTGPCTAAILAAGIARV
                     AYAMEDPNPAVSGRGARRLRDAGLVVHRGLMEAEARELNRGFCRWVVSGKPFVTLKLA
                     VSLDGQIAAAGGDSRWITGEAARRRVHRMRSAADAVLVGGETVRRDDPILTARVPGGH
                     DPRRVILTSHPAELVRRKIFRESGGQVIVACPKGVPEHDVRLVQDAGGMVLRLPARGG
                     AVRARDFLAALGAEGVTSLLVEGGGRIAGWLAAEGAIDRYVVFVAPLLLGEGIRAVSG
                     WASRSPSAGKRLAFTSVRRVGPDIEITAEPRP"
     gene            9717..10352
                     /locus_tag="VF357_03145"
     CDS             9717..10352
                     /locus_tag="VF357_03145"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX7523172.1"
                     /translation="MFTGIVEDVGTVASLSPLRAGMAITVATSLPMETIAEGDSVAVS
                     GVCLTVAQKGAGTFTADVSKETLSKTTFGAMRPGSKVNLERSLTLSGRLGGHIVYGHV
                     DGTGVIREIRPLGEARVFHIQADPSIMKFMVYKGAVTVDGVSLTVSAARRDGFELAII
                     PITLERTTLGVVRAGERVNLETDIVGKYVLKSLEGGGGGVTLDFLKDHGYS"
BASE COUNT         1777 a         3367 c         3781 g         1584 t
ORIGIN      
        1 ccgcggggtc tgccccgtgt gcgggaagta ccggaacctg gaggaatgct cctgcgtcgc
       61 gggggatacc ccgggcccgt tcgaaattct caagaacctg aaaggaaaaa aggagtaagc
      121 catgccgaat ccgaaacgac gcggatcgaa atgccgccgg gacaagcggc gcacgcacaa
      181 gaagctgtcc cagccggcgg tgagcatctg cccgcagtgc aaggcgacca agcgccctca
      241 tgccgtttgc ccgacgtgcg ggacctacaa gggtcgggaa gtcatcgcaa aatccgaaga
      301 ctgacaccct cctccacccg atgaaaatcg ccgtcgatgc gatggggggg gaccacgccc
      361 cgcgcgagat cgtgcgcggt gcggttgagt cggctcgttc caacggcctc tccctgatcc
      421 tggtcggcca ggaggagcgg atccgggcgg agttgcgcga gatcgacgtt tccggcgccg
      481 acatccaggt actgcacgcc tccgaagtcg tggagatgtg cgaagtcccc ggcatcgctc
      541 tccggaagaa acgggactcg tcgatccgcg tcggacttcg gctggtcgcc gacgggaagg
      601 cgtcctcctt cgtcagcgcg ggaaactccg gcgcggtgat ggcggggggg ttcctgatcc
      661 tcaagaagat caacggcgtg gaccgccccg cgatcgcggc gaccatcccc acgccccacg
      721 gcccggtggt ccttgtcgac gcgggcgcga acgtcgagtc caagccggcc catctcctgc
      781 agttcgggta catgggcgag gcgtactccc ggacgatcct ggggatcccg cggccgcgcg
      841 tcggggtcgt cagcatcggc gaggaggatt cgaaagggac ggacctcacg cgggatacgt
      901 gcgaactgtt ccggcgcacc ggcctcaatt tcgtggggaa cgtcgagggg cgggactttt
      961 tcgccgggaa ggccgatgtc ttcgtctgcg acggctttgt cgggaacgtc gcgatcaaga
     1021 cgatggaagg gatggcgacg gcccttggcc agttcctgaa agaggagatc ggcaagtccc
     1081 tcatggcgaa ggtgggcgcc ttcctcgcgg agcgcgcgct acggggggtc aaggaccggc
     1141 tggactacga ggagtacggt ggggctccgc tcctgggggt tcggggcgga gtcttcatct
     1201 gtcacggatc gtccagcgag cgggcgatca agaacgggat ccgggccgcc ggctccctgg
     1261 cgcgctgcgg tgtggacgtc gagatcgcac ggtccatcgc ggcgcggggg cacgccgccg
     1321 gttgaccgcc ggcgatgccg taaagcgatc acgaggggag gggaagcgtt ggcatcgaaa
     1381 atcgtcgggc tggggatgta cgccccgccg aaagagttga ccaatctcga cctcgaaaag
     1441 ctggtcgaca cgaacgacgc gtggatcacg gagcgcaccg ggatccggac ccgccacatc
     1501 gcggagccgg ggacgccgaa ttccgacctt tgcgtcgagg cggcccggaa agcgctcgat
     1561 gacgcgggcg tcgacccggc ggaactcgac atcgtggtca tcgggacccc gacgcccgac
     1621 atgccgttcc cgtccaccgc gtgcttcctc caggcgaaga tcggggcgac gcgcgcctac
     1681 ggcatggacc tctccgccgc ctgttcgggg ttcatctatt ccctgtccgt gaccgacgcg
     1741 ctgatccggg cgggccgggg gaagaaggcc ctcgtggtcg gctccgagat tctttccacc
     1801 gtggtcgatt acaccgaccg gtccacctgc atcctcttcg gcgacggcgc gggggcggcc
     1861 gttctcaccg aatgcccgga aggggagggg atcctaagct gccacctgca ctccgacggc
     1921 aatctctgga agttgatcta ttgcccgggg ggcggcacgc tgcatccgta ctcccccgag
     1981 atggtggaac agcggctccg ctccatccgg atggcgggga atgaaacgtt caagcacgcc
     2041 gtcctgcgaa tggtcgaggt ttcccgcgag gcgctggatc ggagcggggt cggcatcgac
     2101 gacgtgaaac tcttcattcc gcaccaggcg aacctgcgca tcatccaggt cgtcgggaag
     2161 cggctcggga tccccgagga gcgcgtcttc gtcaacctcg agcggtacgg gaacacctcc
     2221 gccgcctcca tcccgatcgc gctcgcggag gccaaggaac agggacggtt cgccgccggg
     2281 gacctcgtgc tcgtcgtcgc gttcgggggg gggctcacct gggcctccgc cctgatgcgg
     2341 atgtaggggg gggcatgggg atcgggctcc tctttccggg gcaggcgtcg cagttccccg
     2401 ggatgggaaa ggatcttcac gacgcgtatc ccgtcgcccg gcgggtcttc gaggaggcgt
     2461 ccgaggcgct ctcgcgggac atggcggccc tttgttttct tggaaccgag gaagagctcc
     2521 ggatgaccga gaacacgcag ccggcgatct tcatcgtgag cgtggccgcc ttccgggtcc
     2581 ttgcggccga aaccggggtc cggcctgcct gcgcggcggg acattccctc ggggaatatt
     2641 ccgcgcttgt cgcggccggc gcgctccccc tgagggaggc ggtccgggtc ttgcggtcgc
     2701 gcgggaaata catgcaggaa gccgtcccgg tgggggaggg cgcgatggcg gcgatcctcg
     2761 ggctatcccc ttcgcaggtc gaggaggcgt gccgtgcggg cgcggcgctg ggcgtcgtct
     2821 cgccggcgaa cttcaacggc ggcggacaga tcgtgatctc gggggaggcg aaggcggtgg
     2881 cggcggcgtg cgaggcggcg aaggccgccg gcgcgaagcg ggcccttcct ctcgccgtga
     2941 gcgccccgtt ccattgcgcc ttgatgcggc ccgcggcgga ccggcttgcg cccgagctgc
     3001 gggcgatccc gcaggggccg ttcgcgttcc cggtggtggc caacgtgacg gcggctccgt
     3061 acggggcggg cgacgtcgtc gccgacatgc tgatccgcca gatcaccgct ccggttcgct
     3121 gggaggaatc ggtcatcgcg atgcgggccg cgggggccga cgcgttcctg gaggtggggc
     3181 cggggaaggt cctttcgggg ctccttcgcc ggatcgggaa ggacgcggcg acggccgtct
     3241 tctgcggccc ggccgacctc gacggggcgc ggggtttgcc cgcctgacgt tttccgctgg
     3301 agccgcggga ccgggacaag gtaagaatac ggggtaagat aggaggaggg ggatgcggct
     3361 gtcggggaag acggcgctgg tgaccggcgc ctcccggggg atcgggcgcg ccatcgccgt
     3421 gcggttcgcc gcggagggcg ccttcgtcgt ggtgaattac gcggggaacg aggcggcggc
     3481 cggcgaaacc ctcgcggcga tcgcctcggc gggggggaac gccgttctct cgcggttcga
     3541 cgtcggagac gccgcccagg tcgacgccgc ggtcaaggcg atcgtcgcgg agcggggccg
     3601 gatcgacatc ctcgtgaaca acgccggagt cacccgggac aacctgctga tgcggctgac
     3661 tgaggatgat ttcgacgccg tcgtgcggac gaacctgaaa gggacgttcc tcgtcacgaa
     3721 ggtcgtctcc cggcagatgc tccggcagcg gtgcgggcgg atcatcaaca tgagctcggt
     3781 ggtgggcgag atggggaacg cgggacagtc ggtctacgcc gcgaccaagg cggggatcct
     3841 cggcttcacc aaggcgatgg cccgggagct cgcttcccgg gaaatcacgg tgaacgcgat
     3901 ctccccgggg ttcatcacga ccgacatgac cgaaaagctg cccgaggcgg cgcgcaagga
     3961 gttttcggaa cggatccccc tcgggcgctt cggcgccccg gaggaggtcg ccgagctcgc
     4021 gctgttcctg gcgtccgacg cggcggcgta cgtgaccggg caggtggtgg ggatcaacgg
     4081 cgggatgtac atgtagcgca taccggacga acgcacacca aggggaaagg gaggaaatgg
     4141 aatgcaggta gaacagaggg ttcgcgagat cgtggcggag caactggaac gggacgtgaa
     4201 cgaggtcacc aacacggcgt cgttcatcga cgacctgggg gcggactccc tcgacatcgt
     4261 cgagctggtg atgaagatgg aagaagagtt cgggatcgag atccccgacg aggaagccga
     4321 gaagatcaag accgtcaacg acgtgatcca gtacatcacg attcacaaga aatagcggcg
     4381 agctccggca ggccccgatc gcaggggcgc gggcggaagg aacgacatgc gcagagtggt
     4441 cgtcacggga ctgggggcgg tgacccccct tggcgtgggt gtccggagca cgtgggacgc
     4501 gattctcgcc ggcacgtcgg gagtggggcc gatcacgcgg ttcgacgcga aggatttttc
     4561 cacgaccatc gcggccgagg tgaaagggtt cgacgccgag cagttcatcg acaggaaaga
     4621 gatcaagcgg atggacccgt tcatccacta cgccatggcg gcggcgcaca tggcgatgga
     4681 ggacgcgagg ctggtgatcg acgacgcgct ggcgccgaag gtgggcgtct acatggggag
     4741 cgggctgggc gggctttcca ccctcgagcg gtaccacaag gcgtacatgg agggcgggcc
     4801 gaggaagatc agccccttct tcatcacgat gctcatctcc aacctcgccc ccgggcacat
     4861 cgcgatgcgg tacggcgcca aggggccgaa catcgccacg acgacggcgt gcgcggcctc
     4921 cagtcacgcg accggcgagg ggatgcacgc gatccgcagc ggggtgtgcg acgtggtgat
     4981 cgcgggaggc gccgaggcga cgatcacccc gctcgggctg ggggggttct gctccatgaa
     5041 agccctgtcc acccggaacg acgatcccgg gaccgcttcg cgccccttcg acaaggatcg
     5101 ggacggcttt atcatgggag aagggtcggc gatcctgatc ctcgaggaac tggagcatgc
     5161 ccggagccgg ggggcgaaga tctacgcgga gttgctcggg tacggagcgt cggccgacgc
     5221 ctaccatgtc acggccccgg ctcccggcgg cgagggagcg gtgcgcgcga tgacggcggc
     5281 cctggccgac gccggggtcc ccgcatcgtc gatagactac atcaacgcgc acggcacttc
     5341 gacgtcctac aacgatctgt acgaaacgat ggcgatcaag accgtcttcg gcgagcgggc
     5401 gaagtcgatc gccgtcagtt cgaccaagtc gatgacgggg catctcctcg gcgccgcggg
     5461 ggcgatcgaa gggatgttct gctccctcgc gctccaggag ggggtgatcc cccccacgat
     5521 gaactacacg acgccggacc ccgagtgcga cctcgactac gttcccaacg cgttgcgccg
     5581 ccaggcgatc cggtacgcca tgtccaactc gttcggcttc ggcggaacga actcggtcct
     5641 gctgttcggc cgtttcgagg cgtagccccg tggtgcccct cacgcgacgc gtcgtcatcg
     5701 cctccgatca cgcgggcgtg gaaatgaagc ggcgtctgcg ggaagcgatg gcagccctcg
     5761 gaatttccgc ggaggacctc gggaccggct ccgtgggatc ggtggactat ccggactacg
     5821 ccgcggcggt cgcccggcgg gtctccaccg gggctgcgga tgtcggcgtg ctcgtctgcg
     5881 ggaccggcat cgggatgtcg atcacggcca acaagttccc cggggttcgc gccgcgctcc
     5941 tctacgacgc cacggcggcg cggttctccc gcctgcacaa cgacgccaac gtggccgtct
     6001 tcggcgcgcg cacgatgtcc gcggacgacg cggcgcggcg gctgcgcctc ttcctgtcgg
     6061 agccgttcga ggcgggcagg cacacccggc ggctcgaaaa gatccgcggc atcgagaaga
     6121 ccatcccggc cggccccgcg ccggccgcaa accgagaggc gcccatgtcc ttcctgaaag
     6181 agaccgaccc cgagatctac gacatcatcc gcaaggagac ggagcggcag gcgtacaagc
     6241 tcgaactgat cgcctccgag aacttcgtga gcgaggcggt cctcgaggcc accgggtccg
     6301 tgctgacgaa caagtacgcc gaggggtacc cggggaagcg gtattacggc gggtgcgagt
     6361 tcgtggacca ggccgaatcg ctggcgatcg agcgcgcgaa gaagatcttc ggcgcggcgc
     6421 acgtcaacgt gcagccccac gccggctccc aggcgaacat ggccgtctac ttcgccgtga
     6481 tgagcccggg cgacacgatg ctgggaatga acctctccca cggcggccac ctgacccacg
     6541 gaagccccgt gaacttctcg gggaagctct acaacgtggt tccctacggc gtgcggggag
     6601 acaccgagac gatcgactac gaccaggtcc gcgacctcgc cctgaagcat cggccgaagc
     6661 tgatcgtcgt gggcgcctcc gcctaccccc ggacgatcga cttcccggcc ttccgcagga
     6721 tcgccgacga ggccggctgc atggtgatgg ccgacatcgc gcacatcgcg gggatggtcg
     6781 ccgtcgggct gcaccccagc ccggttccat actgcgaatt cgtcacgacc acgacgcaca
     6841 agacgccgcg cggcccccgc tccgggctga tcatgtgccg ggaggagttc gcgaagaagc
     6901 tcgattccgc catcttcccg gggagccagg gcggccccct gatgcacgtg atcgcggcga
     6961 aggccgtggc gctgaaggaa gcgatgaccc ccgccttcaa ggagtaccag gcgcaactcc
     7021 tccgcaacgc ggcggcgatg gcgaagatcc tcctcgcccg gggataccgg ctcgtctccg
     7081 gcggaacgga cacccacctg atactggtca acctgaaaga caccccgctt acgggaaaag
     7141 agggggaagg ggcgctggag cacgtgggga tcacggtcaa caagaacacg gtccccttcg
     7201 agacccgaag cccgttcatc accagcggga tccgcatcgg gacgcccgcg gtcaccaccc
     7261 gcgggatgaa ggaaaaagag atggaacgga tcggcaacct gatcgccgac gtgctcgccg
     7321 cgccctcgga cgcctccgtg cagaggcggg tggaggcgga ggtccgcgca ctgtgcgacg
     7381 ccttccctct ctacgcctcg cgtctcgggg cgtacgcgaa agggtgagcc cgttgccggt
     7441 aacggcgcgg acccgtcccg actgggacac ctacttcatg gacatggcga agctcgccgc
     7501 caggcgttcg tcgtgcctgc gtcgggcggt cggcgcggtc ctcgtgaagg accgtcggct
     7561 tctggcgacg ggctacaacg gggttccgtc cggggtcacg cattgcgagg tcgtgggatg
     7621 cctccgcgag cggctcaagg tcccctcggg ggagcgccac gagctgtgcc ggggcctgca
     7681 cgccgagcag aacgcgatca tccaggccgc gtaccacggc gtctcgatcc ggggcgccca
     7741 actgtactgc acgaaccttc cctgcatcat ctgcgcgaag atgctgatca acgccggcgt
     7801 ccgtcggatc atctatctcg aggggtacag cgacaccctg acgcgggaga tgctcgacga
     7861 ggtcgggatg gagctcctga agctcacgga tccctcccca tgaaatgccc ccggtgcggc
     7921 cacgtggaca acaaggtggt cgattcccgg gcgggcaagg acggcgacgt catacggcgc
     7981 aggagggaat gcctctcctg cgcgcggcgg ttcaccacct acgagcggat cgaggaggag
     8041 ctccccctcg tcgtgaagcg ggacggtcgg cgcgagccgt acgaccgcca gaagatcctc
     8101 tccggcatcc gcaaggcgtg cgaaaagcgg cccgtcagct acgacaccat cgaacacctc
     8161 gtggaagccc tcgagcagga attccagtcc ggcgccgaga aggagatctc cacgatccgg
     8221 atcggcgagc gggtgatgtc gaagttgctc caggtggacg acgtggcgta cgtccggttc
     8281 gcctccgtgt accggcagtt caaggacgtg agccagttcg tcgaggagat caagaccctc
     8341 atttccgagc cccccggccg aacgacgaag tcatgaccgc ccgaccggag ttccccggca
     8401 ccgggttcat gcggcaggcg cttcggctcg cccggaaagg ggcggggcgg accgcgccca
     8461 acccggcggt cggcgcggtg gtcgtgcggg gagggcgcgt cgtcgggaaa ggatatcacc
     8521 acgccgcggg gcgtccccac gcagagatcg aggcgctgcg gcgggcgggg agcgcggcgg
     8581 ggggagcgga cctgtacgtg acgctcgagc cgtgcgacca tcgcggacgc acgggaccat
     8641 gcaccgcggc gatcctcgcg gcggggatcg cccgggtggc gtacgcgatg gaggacccca
     8701 acccggcggt ttccggccgc ggcgcgaggc ggctgcgcga cgccggcctc gtcgtgcacc
     8761 gcgggttgat ggaggccgag gcccgggaac tcaaccgcgg gttctgccgc tgggtcgtct
     8821 cggggaagcc gttcgtcacg ctgaagctgg ccgtctccct cgacgggcag atcgcggccg
     8881 ccggcggcga ttcccggtgg atcacgggcg aggcggcgcg ccggcgcgtc caccggatgc
     8941 gttcggcagc cgacgccgtg ctcgtggggg gcgaaacggt acgccgggac gatccgatcc
     9001 tgaccgcgcg cgttcccggg gggcacgatc cccggagggt gattctcaca tcgcaccccg
     9061 cggagcttgt gcgccggaag atctttcgcg aatcgggggg ccaggtgatc gtcgcctgcc
     9121 cgaaaggtgt cccggagcat gacgttcgct tggtgcagga cgcggggggc atggtgctcc
     9181 gcctcccggc gcgcggcggc gccgtgcgtg cgcgcgattt cctggccgca ttgggggcgg
     9241 aaggcgtgac ctccctcctg gtcgaagggg gcggccggat cgccggctgg ctcgcggcgg
     9301 agggtgcgat cgaccggtac gtcgtcttcg tcgcacccct gctcctgggc gaagggatcc
     9361 gtgccgtgtc gggctgggcg agccgatcgc cgtccgccgg gaaacgcctc gccttcacct
     9421 cggtccgccg cgtcggcccg gacatcgaaa tcaccgccga gccgcgcccg tagggaggca
     9481 aatgctttct cattgtttga aatgtacaac gcagggttcc tcgcgggggg gctgcctctc
     9541 cgctacggct cgtcgaccct ccatggtctc ctcgccacgc cgccctacgg aaccccgatg
     9601 cccatggacg ggcaagtgcc gcgggagcca tggatggcgg gagcggcccc gctgcgtcac
     9661 tcctgcgtag caacgcgcct cgttccgaac acagtatcgg gtataaagga gcgctaatgt
     9721 tcaccggtat cgtcgaggat gtggggacgg tcgcctccct gtcgccgctg cgcgcgggga
     9781 tggcgatcac ggtggcgacg agcctgccga tggagacgat cgcggagggg gattccgtcg
     9841 ccgtctcggg cgtctgcctg accgtggcgc agaagggggc ggggacgttc accgcggacg
     9901 tctcgaagga gacgctctcg aagacgacgt tcggcgcgat gcgccccggg tcaaaggtca
     9961 acctggaacg gtcgctcacc ttgtcggggc ggctgggggg acatatcgtc tacggccacg
    10021 tggacgggac cggcgtgatc cgggaaatcc gcccgctggg ggaagcccgg gtattccata
    10081 tccaggccga tccttctata atgaagttca tggtttacaa gggggccgtg accgtcgacg
    10141 gcgtcagcct gacggtcagc gccgcccgcc gggacggatt cgaactggcc atcatcccga
    10201 tcaccctcga acgaacgacc cttggggtcg tgcgggcggg ggagcgggtc aacctcgaaa
    10261 ccgacatcgt cgggaaatac gtcctcaaga gcctcgaagg cggcgggggc ggcgtcacgc
    10321 tggatttcct gaaggatcac gggtattcgt gagaaaggcg aagacgggat gccgctttcg
    10381 aagatcgagg atgccatcgc ggacatccgc gacgggaaga tggtcatcct cgtggacgac
    10441 gaggaccggg agaacgaggg cgacctgacc ctcgccgcgg agttcgtcac ccccgaggcg
    10501 atcaacttc
//
LOCUS       DASVJL010000157         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1618274, whole genome shotgun sequence.
ACCESSION   DASVJL010000157 DASVJL010000000
VERSION     DASVJL010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618274"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1925)
                     /locus_tag="VF357_03150"
     CDS             complement(114..1925)
                     /locus_tag="VF357_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX7523173.1"
                     /translation="MKQAMAKDTDEICQRFGNDRTRMMDIVLEVQARFGCVSHEAMEQ
                     IARAVNTHRVEVESLVTFYSFLSVKPQGQVVIRLCSDIVDWMKGYEDVADAMSKELGV
                     TMGETTPDGAITLTHTPCIGMSDQAPAALVNDVVVTHLTPERGRQLIRDLRRHLDPQQ
                     LVTMVGDGNNAHPLVRSMVRNNIRMRGPVHFAEFESGSALQKALALEPEAVIREIKES
                     RLRGRGGAGFPAGLKWEMARNAPGRNRYILCNADEGEPGTFKDRVLLTERADMMLEGM
                     TTAGYAVGSDTGIIYLRGEYRYLQTFLESAIEERRRQGLLGTDILGKKGFLFDIRIQL
                     GAGAYICGEETALISSCEGLRGDPRNRPPFPPQNGYLGCPTIVNNVETFCCASRILLE
                     GASWFAAMGSPRTTGTKVLSISGDCRKPGVYEVPFGITAQELVEMTEAENAGSMLIGG
                     PSRQMIGSERFSGRICYDDLATGGAIMIFNRARNKLEIAEHYLDFFIEESCGHCTPCR
                     VGLILLKRAVHKVVVGAGEPSDLTDMRDLSTFIIRTSRCGLGQAAPNPVMGALENFGE
                     LYAGLVAERKPGLLPEFDLSQAVVDAELRAGRQSKYV"
BASE COUNT          337 a          744 c          677 g          415 t
ORIGIN      
        1 ggatgtagat tcccgcccct tcggcggcct tcaggatggt ctgccccttt tcggccgcaa
       61 cggatttccc gtcgatcgtc aaggtgacgc tgctcatcgc tagcctccag gggtcagacg
      121 tatttcgact gccggccggc acgcagctcg gcgtccacta cggcctggga gaggtcgaac
      181 tccggcagca gtcccggctt gcgctccgcc acaagcccgg cgtagagctc gccgaaattc
      241 tcgagcgctc ccatcacggg gttgggggcg gcctggccga gaccgcagcg gcttgtccgg
      301 atgatgaagg tcgacaggtc gcgcatgtcc gtcaggtcgg acggctcacc cgcacccacg
      361 accaccttgt gcaccgccct cttgaggaga atcagcccga cccggcacgg ggtgcagtgc
      421 ccgcagctct cctcgatgaa gaaatcgagg taatgttccg cgatctccag cttgttgcgg
      481 gcgcggttga agatcatgat cgccccgccg gtcgccaggt cgtcgtagca gatccggccg
      541 ctgaaacgtt ccgacccgat catctggcgg ctcgggcctc cgatcagcat ggagcccgcg
      601 ttttccgcct cggtcatctc gaccagctcc tgggcggtga tgccgaaagg cacttcgtag
      661 acgccgggct tcctgcagtc gccggagata ctgagtacct tggtcccggt ggtccggggg
      721 gatcccatcg cggcgaacca ggaggcgccc tcgagcagga tgcgcgaagc gcagcagaag
      781 gtctcgacgt tgttcacgat ggtcgggcac ccgaggtacc cattctgcgg agggaacggc
      841 ggacggttcc ggggatcgcc ccgcaaaccc tcgcacgaac tgatgagcgc ggtctcctcg
      901 ccgcagatgt aggcgccggc gccgagctgg atccggatgt cgaagaggaa cccttttttc
      961 cccaggatat cggttccgag gagcccctgc cgcctccgct cctcgatcgc gctttccaga
     1021 aacgtctgga ggtaccggta ttcgccgcgc aggtagatga tccccgtgtc gcttcccacc
     1081 gcatacccgg cggtcgtcat cccttccagc atcatgtcgg cccgctcggt gaggaggacc
     1141 cggtccttga acgttcccgg ctcgccttcg tccgcgttgc acaggatgta ccgattccgg
     1201 cccggcgcgt tgcgcgccat ttcccacttc aggccggcgg ggaacccggc cccgccgcgt
     1261 ccgcgcagcc gcgattcctt gatttcccgg atcaccgctt cgggctcgag cgcgagggcc
     1321 ttttgcaggg cgctccccga ctcgaattcg gcgaagtgca cgggtccgcg catccggatg
     1381 ttgttccgca ccatcgagcg caccagggga tgggcgttgt tgccgtcccc caccatcgtg
     1441 acgagctgct gcggatcgag gtgccgcctg aggtcgcgga tcagctgccg cccccgctcg
     1501 ggagtgagat gcgtcaccac cacgtcgttt accagcgccg ccggggcctg gtcgctcatg
     1561 ccgatacacg gcgtgtgggt cagggtgatc gcgccgtcgg gcgtcgtctc ccccatggtg
     1621 acgccgagct ccttggacat cgcgtcggcg acgtcctcgt agcccttcat ccagtccacg
     1681 atgtcgctgc agagccggat gacgacctgg ccttggggtt tcacggacag gaacgaatag
     1741 aacgtcacca ggctttcgac ttccacccgg tgcgtgttga ccgcgcgggc gatctgctcc
     1801 atggcttcgt gcgaaacgca gccgaaccgg gcctgcacct cgaggacgat gtccatcatg
     1861 cgggtccggt cgttcccgaa cctctggcag atctcgtcgg tatccttcgc catggcctgt
     1921 ttcaattccg cctccccagg atggactgac gactcgcccg cctcgcatgg gacccgcttg
     1981 tgataccata tactgcatac ggtctgacga ccttaccaaa tggtgggccg ccccccctgc
     2041 gctgtcaatg gatattatcg ccgggaggtg ctagcggatc attgcggaac acagcccggg
     2101 gtcacatggt cccttgtccg ccctgaccac ggcgaagcag ccttcccggc ctctttccgg
     2161 cgcgtttcag gcg
//
LOCUS       DASVJL010000158         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1432474, whole genome shotgun sequence.
ACCESSION   DASVJL010000158 DASVJL010000000
VERSION     DASVJL010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432474"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VF357_03155"
     CDS             complement(<1..416)
                     /locus_tag="VF357_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7523174.1"
                     /translation="MTKAFFDHISTTPLDPRVFEAMRPYFLDQYGNPSSHIHDQGQAA
                     LRAVDQARAEVAALIGAKAGEIVFTSGATESNNLAVLGAAEGAGGGKRHVVVSEVEHF
                     SVMNALVPLRNAGYEVTILPVDRNGVVDPDAVRKALR"
     gene            complement(439..636)
                     /locus_tag="VF357_03160"
     CDS             complement(439..636)
                     /locus_tag="VF357_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX7523175.1"
                     /translation="MANWSMEEALRMALRLEEENFVEYEKSAAEATIPGVKSMFLFLA
                     GEERHHMELIKKKMEQFDVKP"
     gene            839..1408
                     /locus_tag="VF357_03165"
     CDS             839..1408
                     /locus_tag="VF357_03165"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7523176.1"
                     /translation="MKRGTFSHKGIGALPLILALVVVLAGAFLLYWNRQPGPEAGKTG
                     PETATSPSGSRTAPPFSLKDTNGNIYSSTQLAGKPVVINFFATWCPPCKAEIPGFVEV
                     YDKLRSEGFEMVGISLDTDTRGDLPGFLMSNKIGYRILFGDLATAQAYGGVSALPTTF
                     FVGKDGEIKNVHVGYMDKDAFDKEVRKLL"
     gene            complement(1496..1666)
                     /locus_tag="VF357_03170"
     CDS             complement(1496..1666)
                     /locus_tag="VF357_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7523177.1"
                     /translation="MAYKITEECIACGVCAPECPVQCIAEGDPIYTIDASLCTDCAAC
                     AGVCPSAACVPA"
     gene            complement(1695..1913)
                     /locus_tag="VF357_03175"
     CDS             complement(1695..1913)
                     /locus_tag="VF357_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX7523178.1"
                     /translation="MRPEVEKVLESIRPALQADGGDVELVDIDGGVVKVRLMGACGGC
                     PMATMTLKGGIEAALKERIPAVERVESV"
     gene            complement(2007..>2535)
                     /locus_tag="VF357_03180"
     CDS             complement(2007..>2535)
                     /locus_tag="VF357_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HEX7523179.1"
                     /translation="LRDGERLVGILSERDIKQASPSPATALEIREIYYLLDKVKVKQI
                     MTRRPYTISSSAPIEEAAMIIREKKIGCLPVVDDGKLIGILTETDIIDSFIEAMGVNG
                     PGYRIEIALANKPGMLFEVLKLLKDFDANIVSVATSPHDDPGRKVFVLRIEARNYKVV
                     KAAIKKSGFDLLSAD"
BASE COUNT          448 a          820 c          765 g          502 t
ORIGIN      
        1 cgaagcgcct tccggacggc gtcggggtcg accacgccgt tccggtccac gggaagaatg
       61 gtcacctcgt accccgcgtt gcgcagcggg accaatgcgt tcatcacgga gaagtgctcg
      121 acttccgaca cgacgacgtg ccgcttcccg ccgcccgcac cctccgccgc gcccaggacg
      181 gcaagattgt tcgactcggt ggctcccgag gtgaagacga tctctcccgc cttggccccg
      241 atcagcgccg cgacctcggc cctcgcctgg tcgaccgccc gcaatgccgc ctgcccctgg
      301 tcgtggatgt gggaggacgg gttgccgtac tgatcgagga agtacggacg catcgcctcg
      361 aagacgcggg gatcgagggg ggtggtcgat atatggtcga aaaaggcctt cgtcatcgga
      421 tcgtccgcgc tctccgggtc acggcttgac gtcgaactgc tccatctttt tctttatgag
      481 ttccatgtgg tggcgctcct ccccggcaag aaagaggaac attgacttca cgccggggat
      541 ggtggcctcg gcggcgcttt tctcgtactc cacgaagttc tcctcctcca ggcgcagcgc
      601 cattcgcaac gcctcttcca tggaccagtt cgccatctcg ccctcctccg ctggccgttc
      661 tccgtgtttg atttccgcga aggcgattcc gtttcaatcg gagtctcccc ggatcctcat
      721 catagcatgg cgggcggcgg tggacgcccc gccagttggt cgcaccgaag caggcgagaa
      781 tcggatttgc tatcatgtaa aactaaactg attaatatcg ggacatggga gacgtggaat
      841 gaaaagaggt acgttttcgc acaaagggat cggggcgttg ccattgatcc tggcgctggt
      901 cgtggtgctg gcgggggcct tcctgctgta ctggaatcgg cagccgggac cggaggccgg
      961 gaagaccggg ccggaaacgg cgacatcgcc ttccggatcg aggaccgctc ctccgttctc
     1021 cctcaaggat acgaacggga acatctactc ctccacccaa ctcgcgggaa agccggtggt
     1081 catcaacttc ttcgcgacct ggtgcccccc ctgcaaggcc gagatccccg ggttcgtcga
     1141 ggtgtacgac aagctccgat cggaggggtt cgaaatggtc ggcatttcgc tcgacaccga
     1201 cacgcggggg gacctgccgg gattcctgat gagcaacaag atcggataca ggatcctgtt
     1261 cggggacttg gccaccgccc aggcatacgg cggggtctcc gccctcccga ccaccttctt
     1321 cgtggggaag gacggggaga tcaagaatgt ccacgtcggc tacatggaca aggacgcctt
     1381 cgacaaggaa gtgcggaagc tattgtagga atggaaaggg gggcgaaaag gtgttctttc
     1441 gcccccctct gatgccggcg tccgccggcc aggtttcgcc ccggtgacgt tcgcgttacg
     1501 ccgggacgca cgccgcggaa gggcacaccc ccgcgcacgc cgcacaatcc gtgcacaagc
     1561 tggcgtcgat cgtgtagatt gggtccccct ccgcgatgca ctggacgggg cactccggcg
     1621 cgcatacgcc gcaggcgatg cactcctccg tgattttgta cgccatgaaa cctccctccg
     1681 aatgagttag tgggttatac ggattccacc cgttcgacgg ccgggatccg ctccttgaga
     1741 gccgcctcga tgccaccctt caacgtcatg gtggccatcg ggcaaccacc gcatgcgccc
     1801 ataaggcgca ccttcaccac accgccgtcg atgtcgacga gttccacgtc tcccccatcg
     1861 gcctgaaggg cggggcggat gctttccaac actttctcga cttctggtct cacgggtaca
     1921 cctcctggat ttcctgctgc ctcgtcatca ctccgccatc atgaacatag gatgccgggg
     1981 ggcgttccgc cgttccttga cgcgggtcaa tcggcggaaa gaagatcgaa ccccgacttt
     2041 ttgatcgcgg ccttcaccac cttgtagttc ctggcctcga tccgcaggac aaacaccttt
     2101 cgccccggat cgtcgtgcgg cgaggtggcc accgacacga tgtttgcgtc gaaatccttc
     2161 aggagtttca gcacctcgaa gagcatcccg ggcttgttcg ccagcgcgat ttcgatccgg
     2221 taccccgggc cgttgacgcc catcgcctcg atgaaactgt cgatgatgtc ggtctccgtg
     2281 aggatcccga tgagtttccc gtcgtccacc acgggcaggc agccgatctt cttctcccgg
     2341 atgatcatgg ccgcctcctc gatcggggcc gaggaggaga tggtgtacgg ccgccgcgtc
     2401 atgatctgct tcaccttgac cttgtcgaga aggtagtaga tctcccggat ctcgagcgcg
     2461 gtcgccgggg aaggggacgc ctgcttgatg tcccgctccg agaggatgcc gacgagccgc
     2521 tcgccgtctc tcagg
//
LOCUS       DASVJL010000159         3670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1526378, whole genome shotgun sequence.
ACCESSION   DASVJL010000159 DASVJL010000000
VERSION     DASVJL010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526378"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1721
                     /locus_tag="VF357_03185"
     CDS             <1..1721
                     /locus_tag="VF357_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7523180.1"
                     /translation="SHTDANFERCVEIALENHEHVTLAVGELLPGIAYLVRRLLENAS
                     NEGFLQRAFHRHVPPVDLLAGPESHVKAPRDREEEGLKPFSNEPVADFTIRETRAAFR
                     NALAGVENRMRESYPAVIGGKEYREGEPIVSVDPARPREVVGTACGITRELADHAVRA
                     AREAQKGWLRRSPGDRAAVLFRAASVARRRRGELAAWQVREVGKSWLEADADVAEAID
                     YLEYYGREMIRLGKAARLGDCPGEENLYLYRPRGVGLVIAPWNFPLAISVGMASAALV
                     TGNAVLYKPSSLSPVNGWLAFSLLREGGAPDGALNFIPGKGDAVGDRLVGHPEVDFVL
                     FTGSRAVGLRIVEKAGRTEPGQKSVKRAVVEMGGKNAILIDADADLDQAIPAVISSAF
                     EYQGQKCPACSRVIVHADCYDRFLARLCEAVKGLTVGSPEDPANGIGPVIDAVSKERI
                     QATIELGRREGKVAAEIPVPGEGYYVSPTVLTDLPPDSKILREEIFGPVLAVIRAGDI
                     EEAVGMANSSDYALTGGLFSRSPATIRRVGETFEVGNLYLLQFMEPRVITENTMRRGF
                     SPDVLP"
     gene            complement(1749..2861)
                     /locus_tag="VF357_03190"
     CDS             complement(1749..2861)
                     /locus_tag="VF357_03190"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7523181.1"
                     /translation="MSCLVMCIVLSASAIVAWGDRGLIVDRFAAKEFAQLPDGVRFPE
                     GIAANPDTGELFVATFDFSGNNKLLRFKSNGKLVAQKDFGASPLLGLAFNLADGKVYI
                     CNTGDLVGLQSKIQRIPADFHEGTPIEDVALLPHIGAPPDRTVGNPDGSQDVIHFGNN
                     AAAPNGMAFHSNGSLFVSDSFQGAIFQIENVDLCPGSCPVTTFVHDALLATTGFPPFG
                     ANGIAFSTDEKSLFIANTGDDRILRMDPGTKKVEVFAESINGADGIAFDSSGHLWVAA
                     NQADEVVVLNGNGRVVATLGDFLGIREDGAPRGLLFPASIVFSKDSAFVTNLALPLTP
                     AVGDEPEEDVRVYTISRIKVPESFVNHRGRGPGDHH"
     gene            complement(3013..>3670)
                     /locus_tag="VF357_03195"
     CDS             complement(3013..>3670)
                     /locus_tag="VF357_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7523182.1"
                     /translation="FDMTAACTGATEKALRKAGVPHEKVYLHPYSHATYYPGARMMHL
                     KLLFTQEGRILGAQIVGHDGVDKRIDVLATAMQAGLTVYDLQELELAYAPPYGSAKDP
                     VNFAGFVASNLLRGETQVVHADALPVDSLLLDVREPAEHEAGALPGSRLMPLGKIRGR
                     LSELPRDRDIVVYCAVGIRGYIAERILRQRGFRAWNLSGGYTTWKQFNPGEEAVQVTG
                     "
BASE COUNT          672 a         1136 c         1179 g          683 t
ORIGIN      
        1 aatcgcacac cgacgcgaat ttcgagcggt gcgtggagat cgccctggaa aaccacgagc
       61 acgtgactct tgccgtcggg gaacttttgc ccggaatcgc gtacctggtg cggcgcctgc
      121 tggaaaacgc ttcgaacgaa gggttcctcc agagggcgtt tcaccgccat gttcctccgg
      181 tagacctcct cgcggggccg gaaagccatg tgaaggcgcc gcgcgacagg gaagaggaag
      241 gattgaagcc gttttccaac gagccggtcg ccgatttcac gatccgggaa acccgtgccg
      301 ccttccgcaa tgccctcgcg ggggtggaga accggatgcg ggagagttat cccgcggtca
      361 tcggcggaaa ggagtatcgg gagggggaac cgatcgtctc cgtcgacccg gcccgcccgc
      421 gggaagtcgt ggggacagcc tgcggcatca cgcgggagct cgccgatcac gcggtgcggg
      481 ccgcccgcga ggcgcaaaaa ggatggttgc gacgttctcc cggggaccgg gcggcggtcc
      541 tgttccgtgc cgcttccgtg gcgcgccggc ggagagggga gcttgcggca tggcaggtgc
      601 gcgaagtcgg gaagagctgg ctggaggcgg acgccgacgt ggccgaagcc atcgactacc
      661 tggaatacta cggccgggag atgatccgcc ttggcaaggc cgcacggttg ggcgactgcc
      721 ccggggaaga gaatctctac ctgtaccgtc cgcggggcgt gggtcttgtc atcgcgccgt
      781 ggaattttcc tctcgccatc tcggtcggga tggcctccgc ggcgctggtc accgggaacg
      841 ccgtcctcta caagccatcg agcctctctc ccgtcaacgg ctggctcgcg ttctccctgc
      901 tgcgggaagg cggagcgccc gacggcgctc tgaatttcat ccccggaaag ggtgacgccg
      961 tgggggatcg cctcgtcggc catccggagg tcgatttcgt cctcttcacc gggtcccggg
     1021 ccgtgggcct gcggatcgtg gagaaggcgg gacggaccga accgggacag aagtcggtca
     1081 agcgggccgt cgtggagatg gggggcaaga acgcgatcct gatcgatgcg gatgccgacc
     1141 tggaccaggc gattccggcg gtcatctcct ccgcgttcga gtaccaggga cagaaatgcc
     1201 ccgcctgctc ccgggtgatc gtccacgcgg attgctacga ccgcttcctg gcccggctat
     1261 gcgaggcggt caaggggctg acggtgggct cgccggagga ccccgccaac gggatcggcc
     1321 ccgtgatcga cgccgtctcg aaggagagga tccaggcgac catcgaactc gggaggcgcg
     1381 aggggaaggt ggccgcggaa ataccggttc ccggcgaagg gtattacgta tccccgacag
     1441 tcctcacgga tcttcccccg gattcgaaga tcttgaggga ggaaattttc gggccggtgc
     1501 tcgccgtcat ccgtgccggg gatatcgagg aggccgtagg catggcgaac tcctccgact
     1561 atgcattgac gggcgggctt ttctccagga gcccggcgac gatccgacgc gtcggggaga
     1621 cgttcgaggt cggcaacctt tatctcctgc agttcatgga gccgagggtc atcacggaaa
     1681 acacgatgcg aagggggttt tccccggacg tcctgccttg atcattcctg gtggttccgg
     1741 gaaatgttct aatggtggtc ccccggtccc cgtccccgat gattcacgaa agactccggc
     1801 accttgatcc gggaaatcgt atatacgcgg acatcctcct cgggttcgtc ccccacagcg
     1861 ggagtcagcg gaagagcgag gttcgtcaca aacgcgctgt cctttgaaaa gacgatactg
     1921 gctgggaaga ggaggccccg gggcgcacca tcctcccgga tgccgaggaa gtcgccgagg
     1981 gttgcgacca cccgcccatt cccgttcagc accacgacct cgtccgcctg gtttgcagcg
     2041 acccagaggt gtccggaaga gtcgaaagcg attccgtcag ccccgttgat gctttcggca
     2101 aatacctcca cctttttcgt cccgggatcc atacggagaa tccggtcgtc cccggtgttg
     2161 gcaatgaaaa ggctcttctc gtcggtgctg aaagcgatcc cgtttgcccc aaagggggga
     2221 aatcccgttg tcgccaacag cgcgtcgtgc acgaacgtgg tgaccggaca actccccggg
     2281 caaaggtcga cgttctcgat ctgaaagatc gctccctgga acgaatcgga gacaaacaag
     2341 gagccgttgc tgtggaaagc catcccgttc ggtgcggcgg cattgttgcc gaagtggatc
     2401 acatcctggc ttccatccgg gttccccacg gtccggtctg gcggggcgcc aatgtgcgga
     2461 aggagggcca cgtcctcgat cggggtgcct tcatgaaaat ccgcggggat gcgctggatc
     2521 ttcgattgga gtcccaccag gtcccccgtg ttgcagatgt acaccttgcc gtccgccagg
     2581 ttgaatgcaa gacccagcaa cggtgacgcc ccgaaatcct tttgcgcaac cagctttccg
     2641 ttcgatttga atcgaaggag cttgttgttc ccactgaaat cgaaagtggc cacgaagagt
     2701 tccccggtat cgggattggc tgctatccct tcggggaacc gtacgccatc gggaagctgc
     2761 gcgaactcct tggcggcgaa ccggtccacg atgagccctc tgtcacccca tgcaacgatt
     2821 gcagaagccg acaggacaat gcacataacc aggcaagaca tcgtctttcg catgttctcc
     2881 cccctctcgg ataataaggt tcacccactt cgatccgttc gacgcaccgc gctttcctct
     2941 ttgattattg tgatggtgcg ctatcgtcgc aggtttcacc ttgaagagca cctcccggga
     3001 gtcgaacaag cctcagccgg tgacctgcac cgcctcctcc ccggggttga actgcttcca
     3061 cgtcgtgtag ccgccgctca ggttccatgc ccggaaccct cgctgcctca ggattctctc
     3121 cgcgatatat ccccggatgc ccacggcgca gtagacgacg atatcgcggt cccgcgggag
     3181 ttcggagaga cgcccacgga tcttccccag cggcatgagc ctggagccgg gaagcgcgcc
     3241 tgcttcgtgt tccgccggct cgcggacgtc gagcaacaga gagtccactg gaagagcgtc
     3301 cgcatggacc acctgtgttt cgccgcgcag aaggttggac gccacgaatc cggcgaagtt
     3361 gaccgggtcc ttggcggaac cgtacggggg agcgtaggca agctccaatt cctgaaggtc
     3421 gtacacggtc aacccggctt gcattgcggt ggcgagcacg tcgatccgct tgtccacgcc
     3481 gtcatgtccg acgatctggg cgccgaggat ccgcccttcc tgcgtgaata gcagcttgag
     3541 atgcatcatc cgcgcgcccg gataataggt ggcgtgcgaa tacggatgga ggtatacctt
     3601 ttcgtgcggc acgcccgcct tccggagagc cttttccgta gcgccggtgc aggctgccgt
     3661 catatcgaac
//
LOCUS       DASVJL010000160         6086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1244737, whole genome shotgun sequence.
ACCESSION   DASVJL010000160 DASVJL010000000
VERSION     DASVJL010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244737"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VF357_03200"
     CDS             complement(<1..924)
                     /locus_tag="VF357_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX7523183.1"
                     /translation="MSVAPIAITGMGCICAAGWNLPDSMEALFRGERHPAPPVRFTSS
                     HPVRYPVFEAADFEEPPDLLRTSAFGLHAAREAVADAGLDRKTLGALRVGVCVGTTVG
                     TVLSEETFCRGYLAGHVPDMTPIRRILRSNPADVIAREFDLTGPRQTVVTACASSTDA
                     VGIAGSWIRADLCDAAIAGGADELGRITYIGFISLMITDDSPCKPFDQHRKGLNLGEG
                     AAMLVLASERILRRSGMRARSFLLGYGSAGDAYHLTAPQPDGAGLRSAISEAMATSGV
                     SSTSLAFVNAHGTGTPDNDRVESRVLHDMLPG"
     gene            complement(921..2375)
                     /locus_tag="VF357_03205"
     CDS             complement(921..2375)
                     /locus_tag="VF357_03205"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7523184.1"
                     /translation="MLNPGRVLLVHPLGYRADAASSDISRIANIMPPLGLASIAASLE
                     RRGIRATIVDCYARPGSDRVIRDHLLAERPAFLGLSCTTSSFLDGIRIAEAARAALPG
                     IRTVFGGPHVSALKEALFPRFPAMDFAVVGEGEDAMGELIESGGEGPGNIPGILFREG
                     REGIARFSGFRDREIELDTLPFPAYEKLDGFPDSYKLPIFNYPRVPNTSCISSRGCPY
                     SCSYCDRSVFRRGFRFNSAEYLYEHLRCLKERFGIRHVNFYDDQFTFHRMRVEAFSGM
                     MIDRPLGMTFNCAVRAEHVDPELLRRMKAAGCWMVSLGIETGDERLLAEHRQHADLDR
                     IAGTIRMIKKAGIRTKGLLMMGLPGETEASIRRSMEYVFSLPIDDFNLTKFTPFPGSP
                     IYETIRELGDFDEDWEKMDCMHFQFVTKGMTAERLEECYRDFYRSHFKRPSVLIGYAA
                     MLWRSPDSWLRFARNMGDFLKFTRTNRRLMDEPG"
     gene            complement(2422..3687)
                     /locus_tag="VF357_03210"
     CDS             complement(2422..3687)
                     /locus_tag="VF357_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523185.1"
                     /translation="MKMTLVYPSWRKLDRQTEFHLPPHGPVVFAATVPPEVSLTFVDE
                     NLQEIDFGAACDLVGISVMLTCQLPRAFEIARRYRERGVPVLFGGIAAMLHAEEVAAH
                     ADSIFLGEAEGRFAGVLDDFSAGRLKKTYDYMNSPPDMALIGTARREILSRDLYNYRG
                     VQMLDLVHASRGCRFNCYPCCSAFLGGKTFRPRPIDRVIEEMEKIRNNRLFIVDNSLA
                     QDRQWLIDLFTAMAPLKKKWVSHPILDDDRVLKLAADAGAWYVYQAVIDTSDLIRNRI
                     RRLKDHGIAVEGTILLGTDEQDEDSIRRLVDFLLEIGLDVAEFTIMTPFPHSPLRTRI
                     AAENRMLSDDWGEYTADRVVFRPRRMSPEKLQEMYHYAWDAFYSGGGHQIKMAELFAK
                     VIRREMEDGTYYRYNPRRERSFPKPADRP"
     gene            complement(3684..4406)
                     /locus_tag="VF357_03215"
     CDS             complement(3684..4406)
                     /locus_tag="VF357_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX7523186.1"
                     /translation="MTGSVVAGIGWVTSQGPGRGRKAREFSMTPGPIPPVTREDVFPE
                     PNLRFGRLPQYSRVGLAAVAFALRDAGMEHWETKRSAGVVASTRAGCLATDIEYYNTV
                     LPVGGGMPSPNLFSSTLPTCFLGDAAIQFGLTGPALIVNDPAEDALGGVRMALESLSW
                     GECGTMLAGHIDLPAEPEIPGIRSPYAGGLFMVLKNSRDSAASGYGALDLSEGGAVRH
                     EGIEIADMNELVRACLAFPRPS"
     gene            complement(4403..5533)
                     /locus_tag="VF357_03220"
     CDS             complement(4403..5533)
                     /locus_tag="VF357_03220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX7523187.1"
                     /translation="MNEVLITATASVTGLGPDLDTTWRRLLAGETSVRPIRRFDAGRY
                     PARNASWIEGLESRGNRSLLYSLLERLLDGFGPLPQGCRLVTATTKGCIDTFERVRKG
                     LPGDVADIPVSGPLTWLASRLGLNGPGVNISCACASSTMAVARAAAMIESGRADAVLV
                     VCMDLVSEFVFSGFSSLQALDPAGCRPFDRNRNGLSLGEGGAALLMMSAESARGKGIR
                     GIGSVVGWGAANDATHVTAPARDGCGLIQAVRRAMAKASIGPDDISAINAHGTGTVYN
                     DAMEITAFEAVVGSRRIPLNSLKGAIGHTLGAAGGIEVAVGLRSLADRTLPPTAGFGE
                     PMEQCGFLVSPLPQPIGGRYLLSTNSGFGGINVALILKGNPE"
     gene            complement(5530..5991)
                     /locus_tag="VF357_03225"
     CDS             complement(5530..5991)
                     /locus_tag="VF357_03225"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX7523188.1"
                     /translation="MITPYFTTEEGAPAPLRAALSRRVRFEEVDPLGIVWHGRYASYF
                     EDARVALTDRYGIGYLDLYEKGVVAPIRKFHVDYFLSLRFRETFTVEAVLHWTEVPRL
                     NMEFTIRNEAREVTTTGYTVQMMLDAGRNLLMLPPPFYREFLDRWRKGKLA"
BASE COUNT         1073 a         1931 c         2033 g         1049 t
ORIGIN      
        1 gccgggcagc atgtcgtgga ggacacgact ctcgacacgg tcgttgtcgg gcgtgccggt
       61 accgtgggcg ttgacgaacg ccaaggaagt ggaggaaacg ccgctcgtcg ccatggcttc
      121 cgagatcgcg cttcgcagcc ccgcgccatc gggctgcggg gcggtcaggt ggtaggcgtc
      181 gcccgccgat ccgtagccga gcaggaagga acgcgcgcgc attccgctcc gccggaggat
      241 ccgctccgag gcaaggacga gcatcgccgc cccttccccg aggttcaatc ccttgcggtg
      301 ctgatcgaac ggcttgcacg gggaatcgtc ggtgatcatg agggagatga atccgatgta
      361 ggtgattctt ccaagttcgt cggcgccgcc cgcgatcgcc gcatcgcaga ggtctgcccg
      421 gatccacgag ccggcgattc ccaccgcgtc ggtgctggac gcgcaggcgg tcaccacggt
      481 ctggcggggg cccgtgaggt cgaattcccg ggcgatgaca tccgccggat tgctccggag
      541 gatccgccgg atgggcgtca tgtccggaac gtgtccggcc aggtacccgc ggcagaaggt
      601 ctcctcgctc aggacggtgc cgacggtcgt tcccacgcag actcccaccc gcaacgcgcc
      661 cagtgttttc cggtcgagcc cggcgtcggc gaccgcttcc cgggccgcgt gcagtccgaa
      721 ggcgctggtc cggaggagat ccgggggttc ctcgaagtcc gctgcctcga acacggggta
      781 gcggaccggg tggctgctgg tgaagcgaac cggcggcgcc gggtgacgtt cgccgcggaa
      841 gagcgcttcc atggaatcgg gcaggttcca cccggccgcg cagatgcagc ccatgcccgt
      901 gatggcgatc ggggcgacgc tcatccgggt tcatccatga ggcggcggtt ggtgcgggtg
      961 aatttaagaa aatcgcccat gttccgcgcg aatcggagcc agctgtcggg cgatcgccac
     1021 agcatggcgg catatccaat caacacactg ggcctcttga agtgggaacg ataaaagtcc
     1081 cggtaacatt cctcgagccg ctccgcggtc atccccttcg tgacgaactg gaaatgcatg
     1141 caatccatct tttcccaatc ctcgtcgaaa tcgcccagct cgcggatcgt ctcgtagatc
     1201 ggcgagcccg ggaagggggt gaacttggtc aggttgaagt cgtcgatcgg gagcgaaaaa
     1261 acgtactcca tgcttcgccg gatgctcgcc tccgtctcgc ccggcagccc catcatgagc
     1321 agtcccttcg tccggatccc ggcctttttt atcatccgga tcgtcccggc gatgcggtcc
     1381 aggtccgcat gctgccggtg ctcggccaaa agccgctcgt cgcccgtttc gatcccgagg
     1441 ctgaccatcc agcagccggc cgccttcatg cgtcggagca actccgggtc cacgtgctcg
     1501 gcccggacgg cgcagttgaa ggtcatcccg aggggacggt cgatcatcat cccgctaaac
     1561 gcttcgaccc gcatccggtg gaaggtgaac tggtcgtcgt aaaagttgac atgccggatg
     1621 ccgaaccgtt ccttcaggca acgcaggtgc tcgtagagat attcggcgga gttgaaccgg
     1681 aaaccccggc ggaagaccga gcggtcgcaa tagctgcagg aataaggaca gccccggctg
     1741 gagatgcaac tcgtgttcgg cactcgcggg tagttgaaga tgggaagctt gtacgagtcg
     1801 gggaacccgt cgagtttctc gtacgccggg aaggggaggg tgtccagttc gatctcccgg
     1861 tcccggaagc cggagaaccg ggcgatccct tccctgcctt cccggaagag gatccccggg
     1921 atgttcccgg gaccctcgcc cccggactcg atcagttccc ccatcgcatc ttccccttcc
     1981 ccgaccacgg cgaagtccat cgccgggaag cgtggaaaga gcgcctcctt cagggcggaa
     2041 acgtggggac ctccgaagac ggtacggatg ccgggcagcg ccgcacgggc cgcctccgcg
     2101 atccggattc cgtccagaaa gctcgatgtg gtgcagctga ggccgaggaa ggcgggacgc
     2161 tccgcgagga ggtggtcgcg gatcacgcgg tcggaaccgg ggcgggcgta gcagtcgacg
     2221 atggtggccc ggatgccccg tcgttcgagg ctggccgcga tgctcgccag cccgaggggc
     2281 ggcatgatgt tggcgatccg cgatatgtct gaggaagcgg cgtcggcgcg atacccgagg
     2341 ggatggacca gcaggacccg tccgggattc agcatcccgg atccttcgga gagacgttgg
     2401 acgatacgag gagattccgc ttcatgggcg atccgccggt tttgggaagg acctttcccg
     2461 acggggattg taacggtaat aggtaccgtc ttccatctcc cgccggatca ccttcgcgaa
     2521 aagctcggcc atcttgatct ggtgaccgcc gcccgaatag aaagcgtccc aggcgtagtg
     2581 gtacatctcc tggagtttct ccggcgacat cctgcggggg cggaacacca cccggtcggc
     2641 ggtgtactct ccccagtcgt cgctcaacat ccggttttcc gcggcgatcc gcgtccggag
     2701 gggggaatgc ggaaacggcg tcatgatggt gaactccgcc acgtccagcc ctatctcgag
     2761 caggaagtcc acgagccgcc ggatcgaatc ctcgtcctgt tcgtcggtgc cgaggaggat
     2821 cgtgccttcg acggcgatcc cgtggtcttt caacctccgg atccggtttc ggatcaggtc
     2881 ggaggtgtcg atcaccgcct ggtagacgta ccaggccccg gcgtcggccg ccagcttgag
     2941 gacccggtcg tcgtcgagga tcggatggga gacccatttc ttcttcaacg gcgccatggc
     3001 cgtgaacagg tcgatcagcc actggcggtc ctgggccagg gagttgtcga cgatgaacag
     3061 acggttgttc cggatctttt ccatctcctc gatcacgcga tcgatcgggc gtgggcggaa
     3121 ggttttccct cccaggaagg cgctgcagca ggggtagcag ttgaagcggc atccgcggga
     3181 ggcgtgaacc aggtcgagca tctggactcc ccggtagttg tacaggtcgc gcgagaggat
     3241 ctcccgtcgg gcggttccga tcagcgccat gtccgggggg gagttcatgt agtcataggt
     3301 ttttttcagc cggcccgcag agaagtcgtc caggaccccg gcgaagcggc cctccgcttc
     3361 cccgaggaag atcgaatcgg cgtgcgcggc gacctcctcg gcatggagca tggcggcgat
     3421 gccgccgaag agaaccggga cgccccgttc cctgtatcgt ctcgcgattt cgaaggcccg
     3481 cggcagctgg caggtcagca tgacggagat cccgaccagg tcgcaggcgg cgccgaaatc
     3541 gatctcctgg aggttctcgt cgacgaacgt cagcgacacc tcgggcggca cggtggcggc
     3601 gaagacgacg gggccgtgcg gagggaggtg aaattccgtc tgccggtcca gcttgcgcca
     3661 ggaaggatag acaagcgtca tcttcatgaa ggtctcggaa aggcaaggca tgcgcgaaca
     3721 agctcgttca tgtcggcgat ctcgattcct tcgtgtcgaa cggcgcctcc ttcggacagg
     3781 tcgagcgccc cgtagccgct tgcggccgaa tcccgggaat tcttcagcac catgaagagc
     3841 ccgcccgcgt acggggagcg gatccccggg atctccggtt cggccgggag atcgatatgc
     3901 cccgccagca tcgtcccgca ctccccccag gagaggctct cgagcgccat ccgcaccccg
     3961 cccagggcgt cttcggcggg atcgttgacg atgagtgccg ggcccgtcag gccgaactgg
     4021 atcgccgcgt ccccgaggaa gcaggtagga agggtggagg agaacaggtt ggggcttggc
     4081 attccgccgc ccacagggag gacggtattg taatactcga tatccgtggc gaggcacccc
     4141 gcccgggtcg aggccacgac gcccgcggaa cgtttcgttt cccaatgttc catcccggcg
     4201 tcgcgcaggg cgaacgcgac ggcggcgagt cccaccctgg aatattgcgg aagtcgcccg
     4261 aaccggaggt tcggctcggg gaagacatct tccctggtca cgggggggat cgggcccggg
     4321 gtcatcgaaa actcccgcgc cttccggccc cggcccggcc cctgcgaagt cacccagccg
     4381 atccctgcga cgacggaacc ggtcactcgg gattcccctt caggatgagg gcgacgttga
     4441 tcccgccgaa gccggaattg gtgctcagca ggtatcggcc tccgatcggt tgcgggaggg
     4501 ggcttacgag gaagccgcac tgctccatag gctccccgaa cccggcggtg ggcggaagcg
     4561 tgcgatcggc cagcgaccgc aggcccacgg ctacttcgat tccacccgca gcgcccaagg
     4621 tgtgtccgat ggctcccttg agcgagttca gcggaatgcg tcgggatccg acgacggctt
     4681 cgaatgccgt tatctccatt gcatcattat aaaccgttcc cgttccatgg gcgttgatgg
     4741 ccgatatgtc gtccggtccg atcgacgcct tcgccatcgc ccggcggacg gcctgaatca
     4801 ggccgcaacc gtcgcgggcg ggggcggtga cgtgggtggc gtcgttggcc gctccccagc
     4861 cgacgaccga accgatcccg cggatccctt tcccgcgggc cgactccgcg ctcatcatca
     4921 gcagggccgc tcctccttcc ccgagggaaa ggccgttccg gttccggtcg aaggggcggc
     4981 aaccggcggg atccagcgcc tgaagcgaac tgaagcccga aaaaacgaat tcgctcacca
     5041 ggtccatgca gacgacgagc accgcatcgg cgcgaccgga ctcgatcatg gcggcggccc
     5101 gggcaacggc catcgtcgag gaggcgcatg cacagctgat attgacgccc ggaccgttca
     5161 accccagcct tgacgcgagc cacgtcagcg gcccggagac cggaatgtcg gcaacatcgc
     5221 cgggtaggcc cttccgcacc cgttcgaagg tgtcgatgca acccttggtc gtggccgtca
     5281 cgaggcggca accctgcggc aggggaccga aaccgtccag caaccgctcg agaagcgaat
     5341 acagaaggga acggttcccg cgggattcca gaccctcgat ccaggaggcg ttccgtgcgg
     5401 gatagcgacc cgcgtcgaac cgccggatcg gccggacgga tgtctctccg gcaaggagtc
     5461 tccgccaggt cgtatccagg tcggggccga gcccggtgac cgaggccgtt gcggtgataa
     5521 ggacctcgtt caagcgagct ttcctttccg ccatcggtcg aggaattcgc ggtagaaggg
     5581 ggggggcagc ataaggagat tcctgccggc gtcgagcatc atctggaccg tgtatccggt
     5641 cgtagtcacc tcccgcgctt cgtttcggat ggtaaactcc atgttcaggc gggggacttc
     5701 cgtccagtgg agcacggcct cgacggtgaa ggtttcccgg aaacggaggg agaggaagta
     5761 gtcgacgtgg aacttccgga tcggggcgac gacccctttt tcgtataaat cgaggtagcc
     5821 gatgccgtac cggtcggtga gcgccacgcg ggcatcctcg aaatagctcg cgtaccttcc
     5881 gtgccagacg atcccgagcg ggtcgacctc ctcgaaccgc acgcgtctgg acaacgctgc
     5941 gcgcagcggg gcgggcgctc cttcctcggt ggtgaaatac ggcgtgatca tcggggagtc
     6001 tctccctccg ccagttcgag ttggaaggcg gagaccgttt tcccggctac cgagagcgtc
     6061 gcgtcgcaga tgtccattcc ggaatc
//
LOCUS       DASVJL010000161         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1337860, whole genome shotgun sequence.
ACCESSION   DASVJL010000161 DASVJL010000000
VERSION     DASVJL010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337860"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VF357_03230"
     CDS             complement(<1..903)
                     /locus_tag="VF357_03230"
                     /inference="COORDINATES: protein motif:HMM:NF040244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX7523189.1"
                     /translation="MSEAVALSELPVSRILSALLSRGGEHGELFFEEARSLTVLMEDG
                     RIERVVSGTDAGIGVRLLFRGKTYYAYTNDRSAESLLALSNDLSRYAEGVGVAATLPA
                     SARSARGPTVVRVPPAARGIGEKVALVREVDRIAREFSREVRQVRATWSESLRRIEVA
                     AHPGVFVREEQTYCVLAVQAVAGDDGGLTMGYESAGGTEGLEFLDGGVPSLLARTAAG
                     RAVRALHAAKLPGGRMQVVLSSEAGGTMVHEAVGHGLEADLARRGMSVYKEKLGERIG
                     SPLVSIADDATVPGKRGSYGIDDEG"
     gene            974..>1557
                     /locus_tag="VF357_03235"
     CDS             974..>1557
                     /locus_tag="VF357_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7523190.1"
                     /translation="MRILVATGGLALLLFLALAGASNGYADPIGPRYDARAYAYYLAG
                     YLDSREGNLDGALGSYRKALKYAGDEPDILYEIANIHVKKGRLPEARAELEKALAADE
                     GHTRSRYLLAGILAASGEREKALAEYGRVLKEDPENDDAYLHIATLHAERGEFSQAEE
                     VLGALIARVPDSHLAYYYRGKILAARKKFDEALT"
BASE COUNT          263 a          547 c          502 g          245 t
ORIGIN      
        1 cccttcgtcg tcgatcccgt acgagccccg cttcccgggg acggtggcgt cgtcggcgat
       61 ggagaccagc gggctcccga tccgctcccc aagtttctcc ttgtagaccg acattccccg
      121 ccgggcgagg tccgcctcca ggccgtgccc gaccgcctcg tgaaccatcg tccctcccgc
      181 ttcggaggag aggacgacct gcatccgccc gccggggagc ttcgccgcat gaagcgcccg
      241 gaccgctctc ccggcggccg tgcgggccag cagcgaagga acccccccgt cgagaaactc
      301 gagcccttcg gtcccgcccg ccgattcata tcccatcgtc agcccgccgt cgtcgcccgc
      361 cacggcctgg acggcgagga cgcagtacgt ctgttcctcg cggacgaaga cgcccgggtg
      421 ggcggcaacc tcgatgcgac gcagcgactc cgaccaggtc gcccggacct gccgcacctc
      481 ccgggagaat tccctcgcga tccggtcgac ctcccgcacc agcgcgacct tctcgccgat
      541 cccccgcgcg gccggcggca cacgtacgac ggtcggcccg cgggccgacc gtgccgacgc
      601 gggaagcgtc gcggcgacgc cgaccccttc cgcgtagcgg gagaggtcat tcgaaagggc
      661 gaggagggat tcggccgacc ggtcgttcgt gtaggcgtaa tacgtttttc cgcggaagag
      721 gaggcgaacg ccgatcccgg cgtcggttcc cgaaacgacc cgctcgatcc ggccatcttc
      781 catgaggacg gtgagggacc gggcttcctc gaaaaacagt tccccgtgct ccccgccgcg
      841 ggacagcagc gcggagagga ttctcgaaac gggcaattcg gagagcgcaa cggcctcgga
      901 catcggtcct cctcgcggtt tcccgggtag tgagtatata atacaaacgg ttgatctttt
      961 ctcggaggaa cggatgcgaa tcctcgtcgc cacggggggc ttggccctcc ttctttttct
     1021 cgccctggca ggggcgtcca acgggtatgc cgacccgatc ggcccccggt acgatgcccg
     1081 cgcctacgct tactatctcg ccggatatct cgattcccgt gaggggaacc tcgacggcgc
     1141 tctcgggtcg tacaggaagg cgctgaagta cgccggggac gagcccgaca ttctctacga
     1201 gatcgccaac atccacgtga agaaggggcg gctccccgag gctcgggcgg aactggaaaa
     1261 ggctctggcg gcggacgagg ggcacacgcg gtcgcggtat cttctggcgg gaatcctcgc
     1321 cgcgtcgggc gaacgcgaaa aagcccttgc cgagtacggc cgcgtcttga aggaggaccc
     1381 ggagaacgat gatgcgtacc tacacatcgc caccctccac gcggagcggg gggagttttc
     1441 ccaggccgag gaggtcctcg gcgcactgat cgctcgggtc cccgattccc accttgcgta
     1501 ctattaccgg gggaagatcc tcgcggcccg gaaaaagttc gacgaggccc tgaccga
//
LOCUS       DASVJL010000162         4252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1808176, whole genome shotgun sequence.
ACCESSION   DASVJL010000162 DASVJL010000000
VERSION     DASVJL010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808176"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VF357_03240"
     CDS             complement(<1..587)
                     /locus_tag="VF357_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7523191.1"
                     /translation="MRVALVRYRYSPHGGAERYLDTLCAGLSAAGAEVRILCASWDGA
                     SAGQVGVERVSVPRKPASLRLFLFARAIRAWAANHPGWLLYSLERVPGVEVYRAGDGV
                     HAEWLLRKRALRPGSWPLDYLRPLNIAYLYLERAMFGSPVLRLVIANSNRGREEIVRR
                     FRIPEENIRVVRNGIDIARFLLDRKADARERFRKR"
     gene            complement(589..1710)
                     /locus_tag="VF357_03245"
     CDS             complement(589..1710)
                     /locus_tag="VF357_03245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7523192.1"
                     /translation="MLYLIVDLPLGGAEIHLLNLVRHLDRDRYSPVVLCIGKNGPIGE
                     EIAREGVPVTELGLLQKGGPDRRIAKILREFLRREKISLVHSHLYHANVYGRLAAFRE
                     RIPAVCTIHNTYARPKMHRRLVNRWLARRTARIIAVSGPVRDDILRYDGVDPSRVVVI
                     PNGVDPERFDLPVTRLEAREKLGIPMGRFLIGTVGRLEEQKGLSYLLEAIRILRGGGK
                     DAFLLVVGSGREEARLQEQASRDGIGDAVFFLGARRDVAELYRAMDVFALSSLWEGGP
                     ITLIEAMASGLPVVATPVGIVPEVVQDGVNGFLVPLRDPAMLAEALWRVREDPAMAEA
                     VGREGRVTVRDRYTHRHLAEKVMAVYEEVLSGNPPAGGQ"
     gene            complement(1796..2539)
                     /locus_tag="VF357_03250"
     CDS             complement(1796..2539)
                     /locus_tag="VF357_03250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7523193.1"
                     /translation="MRRIDWGYQETHVAPLEFELADRVIVQSEFGHRSFVEEAFPADR
                     LVTLPRAVDLGEFTPGTEKEGRRFRVLFVGMVCLRKGFLDLARAWKELALPGGELWVV
                     GQVHDEVIPLLGPYRENPTIRFIGHTKGGAADYYANADAFVLPSIIEGSAKTTYEAMS
                     AALPVITTVNAGSVVRDNVDGFIVPIRDPDSIRDKLLFLYENRERAREMGTSGRNRVG
                     EFSWEIYENRLISLYRDLPHRYAAPFSGS"
     gene            complement(2999..3685)
                     /locus_tag="VF357_03255"
     CDS             complement(2999..3685)
                     /locus_tag="VF357_03255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7523194.1"
                     /translation="MTSIPAVPPDVLDAARGFQADQRWHQFALGCLPPPFGNRKLLDV
                     GCGGGGVAARAAGIGWEVTGIDISPKNVEALRQRGFGAEVVDLNGDFPFECASFSCVT
                     FIEVAEHLVRAEHAFHEISRVLSPGGKVLFTTPNNASYRRRLKALKGHPPDDEGYHLR
                     FWVKKQLQSKWEAAGLLLEVGASYGYLPFVDTLTLHKARTGKRRVFRIGTGWEALFAD
                     RFVWLLKKSS"
     gene            complement(3780..>4252)
                     /locus_tag="VF357_03260"
     CDS             complement(3780..>4252)
                     /locus_tag="VF357_03260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7523195.1"
                     /translation="SPVCALVVGEGPQRERIAAGIAGKDLAVAVRMTGYRRDIPEIVA
                     ASDVIVLPSDRFEGVPQVILQSLAMGRAVVASPIGGIPEVVHHEKTGLLCPAGDPSAL
                     ADAIARLLAEPGLRERLGAAGRELVLSRYSSTAMCERTEGFYRYLGSVKEFRAT"
BASE COUNT          867 a         1385 c         1239 g          761 t
ORIGIN      
        1 aaccgcttcc ggaagcgctc cctcgcatcg gccttccggt cgaggagaaa tcgagcgatg
       61 tcgatcccat tccggaccac gcggatattc tcctcgggta tcctgaatcg gcggacgatc
      121 tcctcccgcc cccggttcga attggcaatg acgaggcgaa gcacggggga gccgaacatc
      181 gcccgctcga gatagagata ggcaatgttg agcggacgca ggtagtcgag tggccaactt
      241 cccggacgca aggctcgctt gcggagaagc cattcggcat gaacaccgtc ccccgcccgg
      301 tagacttcca ccccgggaac gcgttcaagg gaatagagga gccatcccgg atggttcgcc
      361 gcccaagcgc ggatggcgcg ggcaaacagg aaaagacgaa gtgaagccgg cttacgggga
      421 acggaaactc gctccacgcc tacctgtccg gcgctcgcac catcccacga ggcacagaga
      481 attcggacct ccgcacctgc ggcggaaagc cctgcgcaga gagtgtccag gtatcgctcg
      541 gcccctccgt ggggggagta cctgtaacgg acaagagcga ctcgcacgtt attgtccgcc
      601 ggccggagga ttgcccgaga ggacctcctc gtagaccgcc atcacctttt cggcaaggtg
      661 gcgatgggtg taccgatccc gcaccgtcac cctgccttcc cgaccaaccg cctccgccat
      721 ggcgggatct tcacgcaccc tccagagagc ttccgcgagc atggccggat cccgtagagg
      781 aacgaggaac ccgttcacgc cgtcctggac aacctccggc acgatcccga ccggcgtcgc
      841 gacgaccggc agacccgatg ccatcgcctc gataagagta atgggacctc cctcccagag
      901 cgaggaaagg gcaaatacgt ccatcgcccg gtacaattcc gcaacatccc ttcgcgctcc
      961 caggaagaag accgcgtctc cgatgccgtc ccggctggcc tgctcctgca gtcgcgcctc
     1021 ttctctcccg ctaccgacca ccagaaggaa tgcgtccttc cctcctcccc tgagtattcg
     1081 aatggcctcg agcaaatatg aaagtccttt ctgctcttcg agacggccca cggtcccgat
     1141 gagaaagcgc cccattggaa taccgagctt ttcccgcgcc tcaaggcggg tcaccggaag
     1201 gtcgaacctc tccggatcta ccccgttggg aatgacgacc acacgggagg gatcgacgcc
     1261 gtcgtaccgg aggatgtcat cgcggacggg tccggacaca gcgatgatcc gcgccgtcct
     1321 tcgggcaagc caccggttga cgagcctacg gtgcatcttc gggcgggcat aggtgttatg
     1381 gatcgtgcag acggcgggaa ttctctcccg gaatgcggcc aaccggccgt aaacgtttgc
     1441 gtggtataga tgggaatgca caagggatat cttttccctg cggaggaatt cccgcagtat
     1501 cttcgcaatt ctccggtcgg gtcccccctt ttgaagcaat cccaattccg tgaccggaac
     1561 gccttccctt gcgatctcct cgccgatggg gccgtttttc cctatgcaga ggactaccgg
     1621 tgaataacga tccctgtcca gatgcctgac caggttcagc aggtggatct ccgcgcctcc
     1681 caagggcaag tcgacgatca gatagagcac tcgggccggt ctcccgccac ctccggccaa
     1741 agatctgtca ccgttcaatc cccgcccccc agcgccgtta gtttcccgga atacatcatg
     1801 acccggaaaa aggtgcggca taccgatgtg gaaggtctcg gtaaagcgaa atcaaccgat
     1861 tttcgtagat ttcccaggaa aactctccca cccgattccg gcccgaggtc cccatctctc
     1921 ttgcgcgctc ccggttttcg tagagaaaca gcaacttgtc ccggatcgag tccgggtccc
     1981 gaattggcac gataaaaccg tccacgttat cccgaaccac ggaaccggcg ttgacggtcg
     2041 ttatgaccgg gagggcagcc gacatggctt cataggtcgt ctttgcgctc ccttcaatga
     2101 tgctcggaag gacaaatgca tcggcattgg catagtagtc ggctgcgcct cccttggtgt
     2161 gtccgatgaa ccggatcgtt ggattttcac ggtaaggtcc aaggagaggg atcacctcgt
     2221 cgtgcacctg gcctacgacc catagctctc caccggggag agccaattcc ttccacgccc
     2281 gtgccagatc caggaatcct ttccgcagac agaccatacc cacaaagagc acgcggaatc
     2341 gtcgcccctc tttctccgtc cctggggtga actccccgag gtctaccgcc cggggcaagg
     2401 tgacaagccg gtcagcaggg aacgcctctt caacgaagga tcggtggccg aattccgact
     2461 ggacgatgac ccggtcagca agttcaaact ccaagggggc cacgtgggtt tcctggtatc
     2521 cccaatcgat tcgccgcaac cagcgtggag ccccctccat cgggaaggga acattccatc
     2581 gctcgtattc ctcccgcaac aaacgccggg tggtaaccgg gtgcggcccg ggtctttcca
     2641 ggacagtgta agccccgagc cgcttggcat cgcggaaaga tcggaggcat tcgttcgtcc
     2701 atccgtggaa gaccgtgcag cctcggcgtt tgacgtaccg ggccgctgcc gcatcgagag
     2761 ccatccgttt gagtgtgtaa taatatctgg ccttcagaaa ggaaagcgct ttcgccggct
     2821 ggaatcggat ggggcggatc aggtttcccg ggatctccga ttgacggttc ccgtagcaga
     2881 tgaccgcatc cagtgccccg gaacgatagg ccgctcgggc ggtgtggaac aggacattcg
     2941 agattcccgt tcctccgacc tttccgaagc acgatatgac aattcccatg ggccctcgtc
     3001 aggacgattt tttcagaagc cagacgaatc ggtcggcgaa gagggcttcc caacctgtcc
     3061 cgatgcggaa gacacgccgt ttcccggtcc tcgccttgtg gagagtcagc gtatcgacga
     3121 aaggaaggta cccatagctg gcgcccacct ccaaaaggag gccagccgcc tcccacttgc
     3181 tctggagctg cttcttcacc cagaaacgta aatggtaccc ctcgtcatcc ggggggtgcc
     3241 ccttgagcgc cttgagacgt cttcgatacg aagcgttgtt cggtgtcgtg aagaggacct
     3301 tcccccccgg agaaaggaca cgcgagatct cgtggaaagc atgctccgcc cggaccaggt
     3361 gctcggccac ctcgatgaag gtgacgcaac tgaacgacgc gcactcgaag ggaaagtcgc
     3421 cgttcaggtc gaccacttcc gcaccgaacc ccctctggcg cagggcctcg acattcttcg
     3481 gcgatatgtc gatgcccgtg acctcccatc caatccctgc ggcccgtgcg gcgacgccac
     3541 ccccaccgca gccgacatcg agcaacttcc ggttgccgaa tgggggggga agacacccga
     3601 gagcgaactg gtgccatcgc tggtcggcct ggaagcctcg tgcggcatcg aggacgtctg
     3661 gcggaaccgc tggtatggag gtcatcgttg gagaaacctc gaatggaagt acggatgccg
     3721 gttcattaaa tcagtaatgg gcgaagaatg gtatggcgga gccgcccgga aaaacccggt
     3781 caagtcgccc tgaactcctt caccgatccc aaatatctgt agaacccctc cgtcctctcg
     3841 cacatcgccg ttgacgagta gcgggagagg acgagctcac ggcccgccgc cccgaggcgt
     3901 tcccggagtc ccggttcggc gagaaggcgc gcgatcgcat ccgcgagggc ggacgggtcc
     3961 ccggccgggc agagaagccc ggtcttttca tgatggacca cttccgggat accgccgatg
     4021 ggcgacgcga cgacggcgcg gcccatcgcc agcgattgca ggatgacctg cggcactccc
     4081 tcgaaccggt cggagggcag gacgatcacg tcggaggccg cgacgatctc cgggatatcc
     4141 cgccgataac cggtcatccg gacggcgaca gccagatcct tcccggcgat cccggcggcg
     4201 atccgttcac gctgcgggcc ttcgcccacg accagagcgc acacggggga gc
//
LOCUS       DASVJL010000163         5049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1618883, whole genome shotgun sequence.
ACCESSION   DASVJL010000163 DASVJL010000000
VERSION     DASVJL010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618883"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VF357_03265"
     CDS             <1..1180
                     /locus_tag="VF357_03265"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX7523196.1"
                     /translation="RRGVPTLSMPQAVARFFIGERESIVVAGTHGKTTATSLAAWSLF
                     DLGADPSFLVGGVPKNFPVSYRLGQGRHFVIEGDEYDTAYFDKGPKFLHYRPRVVLLT
                     SVEFDHADIYRDLDHVRESFRKLAAILPPDGLLVACADYPDVVAVAREARCPVIFYAT
                     RDGALPGGQDETSWAVRGTGESGGMTGFRMEGAGRALDFRFPLPGLHNAANAAGVAIV
                     LMHLGFVPSVIARTFERFAGIRRRQEVVGEFGGILVVDDFAHHPTAVRETIRAVRARY
                     PGRRIVAVFEPRSNTSRRKVFQREFTAALSGADEVILAGVFGVEKIPPEERLSPEEVA
                     AELRASGRPAQVILEVNGIVSRLSETCRAGDLVLVMSNGGFGGIQEKLQNALPANDDR
                     "
     gene            1258..2895
                     /locus_tag="VF357_03270"
     CDS             1258..2895
                     /locus_tag="VF357_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX7523197.1"
                     /translation="MRNIPGRHAAPVVLGAVLLVSCVLASLVPASAAGYPRRIAIAPF
                     ASLTKEDIGGTVSVLPRLLASRLMALAGADVLLLPAGGRSPEEAAREAKYPLLLQGTI
                     SKLGKGYSIDASVTDLSTGRSAGAFFTAAATEDDIIAQLGVLSGEIAEKLFDIQGAIR
                     AVSPAPTAAAPAPVPAAPLAIGGVPSTPSPQASPAAASPPAPAPTTLAGGWSPSSVKG
                     VGQSDKILDALYGVVTVDTDAEGNALVAAYGKTTLHLYRVKGTEILPFTRISKPLDHH
                     ILNVYAMDIDGDGSREILVTDLVNETVESFVLKKKGDTYLEVAGGIHYYLAVLPDWMG
                     KPVLVGQYQGIETPFQGKIVTLRWDGKGFAEGEKLPQNTNILPLASGLPGVSSARFGK
                     EWRLIYTDANSNLRVLDAGGKSQYKSSGQYGSTPDLFEWGPILQLEGHRKWYPVRNAA
                     RVAAGGGEAPTVLIPEIKQSVLGTVSSTRLVLLQWNGGEFVEKAGSQGSGRYLTGADF
                     LSPDGFGKGGKIVASTIEQSGSAFTEKISRLLLFQAE"
     gene            3142..4614
                     /locus_tag="VF357_03275"
     CDS             3142..4614
                     /locus_tag="VF357_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523198.1"
                     /translation="MRKGLIVLLVAVVVVAFALPAMADMTPTNLSVSGFYRSHAFLSN
                     FHDAYSKPSLRTGDPGDEQQTNAFVEQRLRVRFAFGTENVQAIWFLESDNLWGDVAGS
                     SKQFTTDTTGLGAARNSGGALGADRINTETKNVYVWFKVPDTSVEVAVGLQNQTDSYA
                     GVIYGGADMAGIFVNGKLEPVTYKFGWAKLYENDTKKADDMTLYVAEVDLSPVKEVKL
                     GLNFYFLQDDTGKDRTVVFSSTSTGVELPLDPGVNTFKSRVYMPGVDATFKAGPATIS
                     GFAMYQFGKIEAVRTLKKDVDINAYMLDARADLNVGPGKFFVEGLYLSGGDDPATKYK
                     APITLATREASPGGNSAYSRTNMEIMLSSPDTINVSQCLIGCSGGETSTDPGNGGRGI
                     WHVAAGYQQKVTDKLSARVNAGYLSATKTTKADGLAGVKGKDMATEVNARIDYNIAKG
                     LDVGVVGAYAFIGDFYNSNAPGSHDVKDAWTSYARINYAY"
     gene            4775..>5049
                     /locus_tag="VF357_03280"
     CDS             4775..>5049
                     /locus_tag="VF357_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX7523199.1"
                     /translation="MFENLSEKFQGVLKKLRGHGRISEGNVEGALNEVRLALLEADVN
                     YKVVKDFVAAIKEKALGAEVLASLSPDQHFIKIVHEEMTRMMGAQAQ"
BASE COUNT          937 a         1550 c         1667 g          895 t
ORIGIN      
        1 ccggcgcggc gtgccgacgc tgtcgatgcc ccaggcggtc gcgcgcttct tcatcgggga
       61 gcgggaatcg atcgtcgtgg cggggacgca cgggaagacg acggccacgt cgctcgccgc
      121 ctggtccctc ttcgacctcg gagccgaccc gtcgttcctc gtgggcgggg tcccgaagaa
      181 cttccccgtg agctaccggc tcgggcaggg gcggcacttc gtgatcgagg gggacgagta
      241 cgacaccgcg tacttcgaca aggggcccaa attcctccat taccgccccc gcgtcgtcct
      301 gctcacgagc gtcgagttcg accacgccga catctaccgg gacctggacc atgtgcggga
      361 gtcgttccgc aaactggcgg cgatcctccc ccccgacggc ctgctcgtgg cgtgcgccga
      421 ctacccggac gtcgtcgcgg tggcgcggga ggcgcgctgc ccggtgatct tctacgcgac
      481 gcgggacggt gcgcttccgg ggggacaaga tgaaacgtcg tgggcggtcc gagggaccgg
      541 agagtccggc gggatgaccg gtttccgaat ggagggggcg gggcgcgccc tcgacttccg
      601 cttccctctg ccggggctgc acaacgcggc caacgcggcg ggcgtcgcga tcgtcctgat
      661 gcacctcggg ttcgtccctt cggtgatcgc ccggacgttc gagcggttcg cggggatccg
      721 tcggcggcag gaggtggtcg gcgagttcgg cgggatcctc gtggtcgacg acttcgcgca
      781 ccatccgacg gcggtccggg agacgatccg ggcggtccgc gcccggtacc cgggccgtcg
      841 gatcgtggcg gtcttcgagc cccggtcgaa caccagccgc cggaaggtct ttcagcggga
      901 gttcaccgcg gccctttccg gggcggacga ggtgatcctc gccggggtgt tcggggtgga
      961 aaagatcccg cccgaggagc ggctttcccc ggaggaggtg gcggcggagt tgcgcgcgtc
     1021 cgggcgcccc gcgcaggtca tcctcgaggt aaacgggatc gtctcccgcc tctcggaaac
     1081 gtgccgggcg ggcgacctcg tcctcgtcat gtcgaacggc ggcttcggcg gcatccagga
     1141 aaaattgcaa aacgcactcc ctgcgaacga cgatcggtaa tcggaggatc ttgtcatcgg
     1201 aaagccgttt ttcggttttc attgacggag tccttctcct ctgttagatt tcggacaatg
     1261 cgcaacattc ccggtcgaca cgcggctccc gtcgttcttg gcgccgtcct tctcgtatcc
     1321 tgcgttctgg cgtccctcgt cccggcatcc gcggccgggt atcctcgccg catcgccatc
     1381 gccccgttcg cgtccctgac gaaggaggat atcgggggga cggtttccgt cctgcctcgc
     1441 ctgctggctt cccgcctgat ggcgctggcc ggagccgatg tcctgctgct tcccgcaggg
     1501 gggagatccc ccgaagaggc ggcccgggag gcgaagtacc ccctcctgct ccaggggacg
     1561 atctccaagc tcgggaaagg gtacagcatc gatgcctcgg tgaccgacct gtccaccggc
     1621 aggagcgccg gagcgttctt cacggcggcg gcgaccgaag acgacatcat cgcgcaactg
     1681 ggcgtgctct ccggcgagat cgccgaaaaa ctcttcgaca tccaaggggc gatccgggcc
     1741 gtgtccccgg ctcccaccgc cgcggccccg gccccggttc cggccgctcc gcttgcgatc
     1801 ggcggggtcc cgtcgactcc ttccccgcag gcctccccgg cggccgcttc accccccgct
     1861 ccggccccga ccacgctggc cggcgggtgg agcccgtctt ccgtaaaggg cgtggggcag
     1921 tccgacaaga tcctcgacgc attgtacggt gtcgtcacgg tggacacgga cgccgagggg
     1981 aacgccctgg tggccgccta cggaaagacg accctccacc tgtatcgggt gaagggaacg
     2041 gaaatcctcc cgtttacgcg catcagcaag cccctggatc accatattct gaacgtctat
     2101 gccatggaca tcgatgggga cggctcgagg gagatcctcg tcaccgatct cgtgaacgag
     2161 acggtggagt cgttcgtcct gaagaaaaaa ggggacacgt acctggaagt ggcgggaggg
     2221 attcattact acctcgccgt actcccggac tggatgggga agccggtact ggtcggtcag
     2281 taccagggga tcgagacccc gttccagggg aagatcgtga cccttcggtg ggacgggaag
     2341 gggttcgccg aaggggaaaa acttccccag aacacgaaca tcctcccgct ggcgtcgggc
     2401 cttccgggtg tctcgtcggc ccggttcggg aaggagtggc ggctgatcta cacggacgcg
     2461 aattcgaacc tccgggtcct ggatgccggg gggaaaagcc aatataaatc gtccggtcag
     2521 tacgggtcga ccccggatct cttcgagtgg ggtcccatcc tccagcttga gggccacagg
     2581 aagtggtatc cagtgcggaa cgcggcacgc gtggccgccg gcggggggga ggcccccacg
     2641 gtcctgattc cggaaatcaa gcagagcgta ttgggcacgg tttcgtccac gcgcctggtt
     2701 ctcctccaat ggaacggggg ggaattcgtg gagaaggcgg gtagccaggg cagcggccgc
     2761 tacctcacgg gggccgattt cctctccccg gacggtttcg gaaaaggggg gaagatcgtg
     2821 gcctcgacga tcgagcaatc cggatcggca ttcaccgaaa aaatcagccg gttgctttta
     2881 ttccaggcgg aatagcgcgg acagcgtatc cagcgcgtgg aaaaaccttg acaatgcgtt
     2941 ggggggatgg tattcaggtc aaaaaaaaac aaggttttct ttctttaaag ctttggcata
     3001 cttgaggtgc tcagggcgca agaggggcaa gagataagac ttttacggga ggaggtggtg
     3061 gtgaagggga gatcgaaagg acggaatgtt tttcctgctg tgcagtggaa gaataggaac
     3121 tcatctctaa ggaggggaat catgcgtaaa ggtttgatcg ttttactggt ggctgtcgtg
     3181 gtggtggcgt tcgccttgcc ggcgatggcg gatatgaccc cgacgaacct cagtgtcagc
     3241 gggttctacc gctctcatgc gtttctgtcc aatttccatg atgcctacag caagccaagc
     3301 cttagaaccg gcgatccggg agacgagcaa cagaccaacg cgttcgtcga gcagcgcctt
     3361 cgtgtgaggt ttgccttcgg taccgagaac gtgcaggcca tctggttcct tgagagcgac
     3421 aatctctggg gcgacgtggc cggcagctct aagcaattca cgaccgacac gacggggttg
     3481 ggcgcggccc ggaactccgg cggggcgctc ggcgccgacc gtatcaacac cgagacgaag
     3541 aacgtctacg tctggttcaa ggtccccgac acctccgtgg aagtcgcggt gggtctccag
     3601 aaccagaccg actcctacgc gggcgtcatc tacggcggcg cggacatggc gggaatcttc
     3661 gtcaacggga agctcgagcc tgtgacctac aaattcggct gggcgaagct ctacgagaac
     3721 gacacgaaga aggctgacga catgaccctg tacgtcgccg aggtggatct ctccccggtg
     3781 aaggaagtga agctgggatt gaatttctac ttcctccagg acgacacagg gaaggatagg
     3841 accgttgtct tttctagtac cagtaccggt gtcgaactgc ccctcgatcc tggcgtgaac
     3901 accttcaagt ccagggtcta catgcccggc gtcgacgcga ccttcaaggc cggtccggcc
     3961 accatcagcg ggttcgccat gtaccagttc ggaaagatcg aagcggtgag aaccctcaag
     4021 aaggacgtcg acatcaacgc ctacatgctg gatgcgcgcg ccgacctgaa cgtcggcccc
     4081 ggcaagttct tcgtcgaggg gctctacctg agcggcggcg acgatccggc caccaagtac
     4141 aaggcgccca tcacgctggc cacccgggag gcgtctcctg gcgggaactc cgcctacagc
     4201 cgtacgaaca tggaaattat gctctcgagc cccgacacca tcaacgtctc ccagtgcctc
     4261 atcggctgct ccggcggcga gacgagcacc gacccgggca acggcgggcg cgggatctgg
     4321 cacgtcgcgg ccgggtacca gcagaaggta accgacaagt tgtcggcccg ggtcaacgcc
     4381 ggatatctgt ccgccaccaa gacgacgaaa gccgacggcc tcgccggggt gaagggcaag
     4441 gacatggcta ccgaggtcaa cgcgagaatc gactacaaca tcgccaaggg tttggatgtg
     4501 ggcgtggtcg gcgcctacgc gttcatcggc gatttctaca attccaatgc tcctggcagt
     4561 catgatgtca aggatgcctg gacctcctac gcccggatca actacgcgta ctaggcgaag
     4621 cgggaacgga atcctcacga acggcccccg ggggaaaccc cgggggctct tttcttctgt
     4681 aaccactcca ttattgaatt gacggaagcg atgcaaatcc cgtagtgtgc cagtttcgat
     4741 cgcatcccga tgcgcaagga gatccggttc gacgatgttc gagaacctgt cggaaaagtt
     4801 ccagggcgtc ctcaaaaagc tgcgcgggca cgggcgcatc tccgagggga acgtcgaggg
     4861 ggcgctgaac gaggtccgcc tggcgctgct ggaagccgac gtcaactaca aggtcgtgaa
     4921 ggatttcgtc gcggcgatca aggagaaggc gctgggggcc gaggtgctcg cctccctttc
     4981 ccccgaccag cacttcatca agatcgtcca cgaagagatg acccggatga tgggggcgca
     5041 ggcgcagga
//
LOCUS       DASVJL010000164         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_587942, whole genome shotgun sequence.
ACCESSION   DASVJL010000164 DASVJL010000000
VERSION     DASVJL010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587942"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..810)
                     /locus_tag="VF357_03285"
     CDS             complement(31..810)
                     /locus_tag="VF357_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7523200.1"
                     /translation="MVRRDKSNYIIQSVAHALDVLEEFRDETEELGVTELSKKLKLHK
                     NNVFRILATLQSRNYIEQNRANDNYRLGIKCLELGQTFIHQRGLLKQARPILQELAET
                     TGETSYISILRGNEVVYLDAVETSSTVRVVSRVGLHMPIHATAAGKALVSYDSDDEFR
                     KLFSGELPRFTKATRTDVEEFIKEVALVRECGYATDLEEFEEGLRCIGAPVRDYTRKV
                     VGAISVSGPAHRLSDERIATVIGPAIDRAGKALSARLGFRE"
     gene            complement(935..1504)
                     /locus_tag="VF357_03290"
     CDS             complement(935..1504)
                     /locus_tag="VF357_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7523201.1"
                     /translation="MKRSGILLRFALLLALAAALSFPGCKKKQPPAPVGEGESIRLNV
                     AADIENYKEILRKDPNNLQALIGIGNLYFDTNQDLLAIENYRKALAMDPTNTNVRTDM
                     AVCYRRSGNPGQAVEELKKAISTTPRHAQSRYNLGVILIQDMHDVEGGIKAWEALLEN
                     VPDYPYRDNLKAEIAKMRSVQGSGKQVYK"
     gene            complement(1501..1986)
                     /gene="folK"
                     /locus_tag="VF357_03295"
     CDS             complement(1501..1986)
                     /gene="folK"
                     /locus_tag="VF357_03295"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX7523202.1"
                     /translation="MTARREAVLLLGSNQGRRVRRIRDAVDRLSRETELLAVSRMYAS
                     EPFGRSHQPWFLNLAVRAATGQSPWGLLALVKRLELEAGRRGGARWGPRTLDVDILLL
                     GDAVVDLPGLVIPHASMAQRRFCLLPAAEVAPGAVVPTYGRTVTQMLNSCEDPLEVIV
                     L"
     gene            complement(2002..>2459)
                     /locus_tag="VF357_03300"
     CDS             complement(2002..>2459)
                     /locus_tag="VF357_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7523203.1"
                     /translation="QRVDYEAELAVVIGRVAKNVKEDAAAAHILGYTCINDVTARDLQ
                     LKDVQFARAKGFDTFAPLGPWIVTDFDPTEASVRCLVNGEVQQDGNTREMGASVYRLV
                     AYISSIMTLFPGDVIATGTPPGIGPLHVGDVVTVDVGGIGALTNRVVAP"
BASE COUNT          426 a          791 c          777 g          465 t
ORIGIN      
        1 ccttccctca acgggcatgt caaggcgggg ctattcccgg aaaccgaggc gcgccgagag
       61 ggcctttccc gcccgatcga tcgccgggcc gatcacggtg gcgatccttt catcggaaag
      121 ccggtgcgcc ggaccggaaa cactgatcgc gccgaccacc ttgcgggtgt agtcgcgcac
      181 gggggcgccg atgcagcgca gcccctcctc aaactcctca agatcggtgg catagccaca
      241 ctcccggacg agcgcgacct ccttgatgaa ttcttcgaca tcggtccggg tggccttcgt
      301 gaagcgggga agttcgccgg agaacagctt ccgaaactcg tcgtcggagt cgtaggagac
      361 cagggccttc cccgcggcgg tggcgtggat cggcatgtgc aggccgacgc gggagacgac
      421 gcggaccgtg gaggaggttt ccaccgcatc gaggtagacc acctcgttcc cccgaaggat
      481 ggagatgtag ctcgtctccc ccgtggtctc ggcaagctcc tgcagaatcg gccttgcctg
      541 tttcagcaac ccgcgctggt ggatgaaggt ctggcccagt tcgagacact tgatcccgag
      601 gcggtagttg tcgttggcgc ggttctgttc gatgtagttg cgcgactgga gcgtggccag
      661 gatgcggaag acgttgtttt tatggagttt cagcttcttg ctgagttccg tcacccccag
      721 ctcttccgtt tcgtcccgga actcctccag aacatcgagg gcgtgcgcga ccgactggat
      781 gatgtaattc gatttgtctc gacggaccat ggaaatcccc cggtgggtgg aatcgtttca
      841 taacaaaaca atgttagata tatttgatcg gaatgcgcat gtcaacagaa agatagaacc
      901 agattatatt cttcgaatct ccttgtgcgc atgcctactt gtacacctgc ttgccggatc
      961 cctgcaccga gcgcatcttc gcgatttcgg ccttcaggtt atcccgatac ggatagtccg
     1021 gcacattttc gaggagcgct tcccacgcct tgatcccacc ctcgacatcg tgcatgtcct
     1081 ggataagaat caccccgagg ttgtagcggg actgcgcgtg ccggggggtg gtggagatgg
     1141 ccttcttgag ttcctcgacc gcctggccgg ggttcccgct gcggcggtag cagaccgcca
     1201 tgtcggttcg cacgttcgtg ttcgtggggt ccatcgcgag cgccttccgg tagttctcga
     1261 tcgcgagaag atcctgattc gtgtcgaagt agaggttccc gatcccgatc agcgcctgca
     1321 ggttgttcgg atccttccgc aggatctcct tgtaattctc gatatccgcg gcgacgttca
     1381 gccggatcga ctctccctcc cccaccgggg cgggcggctg cttcttcttg cacccgggaa
     1441 acgaaagggc cgccgcaaga gccaacagga gcgcgaaacg gaggagaatg ccgcttctct
     1501 tcataacacg atcacctcga gaggatcttc gcacgagttc agcatctgcg tcacggtgcg
     1561 gccgtacgtg ggaacaaccg cgccgggggc cacttcggcg gcgggcagaa ggcagaatcg
     1621 gcgctgcgcc atcgacgcgt gcggaatcac gagccccggc aagtcgacca cggcgtcccc
     1681 cagaaggagg atgtcgacgt ccagcgtccg tggaccccac cgggctccgc cgcggcggcc
     1741 cgcctccagc tcgagccgct tcacgagcgc gagcagtccc cacggggatt gccccgtcgc
     1801 cgcgcggacc gccaggttga ggaaccacgg ctggtgtgac cgcccgaacg gctcgctggc
     1861 atacatccgg gagacggcga gcagttcggt ttcgcgggag agccggtcca cggcgtcgcg
     1921 gatccgccgg acgcgcctcc cctggttgct tccgaggagg agaaccgcct cgcgccgcgc
     1981 ggtcatccgc cccccccttg attacggcgc gacgacccgg ttggtgaggg cgccgatccc
     2041 gcccacatcg acggtaacga catcccccac atggagcggc ccgatgccgg gaggcgttcc
     2101 ggtcgcgatc acgtccccgg ggaacagcgt catgatggag gagatgtacg ctaccagccg
     2161 gtagacggac gcacccatct cccgcgtgtt gccgtcctgt tggacctcgc cgttcacgag
     2221 gcagcggacg gacgcttcgg tcgggtcgaa atcggtaacg atccacggcc cgagcggggc
     2281 gaacgtgtcg aaccccttgg cgcgggcaaa ctgcacgtcc ttcagctgga ggtcgcgcgc
     2341 ggtcacgtcg ttgatgcagg tgtagccgag gatgtgcgcg gcggcggcgt cctccttcac
     2401 gttcttcgcc acccggccga tcaccaccgc gagctccgcc tcgtaatcca cgcgctgcg
//
LOCUS       DASVJL010000165         5307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_120188, whole genome shotgun sequence.
ACCESSION   DASVJL010000165 DASVJL010000000
VERSION     DASVJL010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120188"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VF357_03305"
     CDS             <1..506
                     /locus_tag="VF357_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523204.1"
                     /translation="DFLFYAVIVDFSLELVDFIHRVYQSEEEIKILSEMVMSKLFGSL
                     IIIQVLLGMLVPLLLISLTKIFKKRFHLNEDLRKMVYFVAVILIQFGIFATRWNVVIG
                     GQMFSKSFRGLTTYKMGFGGLEGLLYAIVLLALPIVILAVLVKVLPPWKEMDGEPASE
                     GVEVAGS"
     gene            650..979
                     /locus_tag="VF357_03310"
     CDS             650..979
                     /locus_tag="VF357_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7523205.1"
                     /translation="MSGADTRRQSQSLRNQALSLLRQAVVHLQYAPGAVVSEAELVVQ
                     VGLGRTPVREAVARLEQEGLVTVLPRKGIQITPVTLVDLQALCELRMELEGWGHGWPR
                     SVVPRSN"
     gene            985..1197
                     /locus_tag="VF357_03315"
     CDS             985..1197
                     /locus_tag="VF357_03315"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX7523206.1"
                     /translation="MQGLFAEAPVLIAANDLSQLLELDRDFHMLLARCTKNTYLEETL
                     SRLYNNSLRYCFAPKCSRRMRGRCGR"
     gene            1197..1481
                     /locus_tag="VF357_03320"
     CDS             1197..1481
                     /locus_tag="VF357_03320"
                     /inference="COORDINATES: protein motif:HMM:NF022463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 family protein"
                     /protein_id="HEX7523207.1"
                     /translation="MDRWDTLQLRSYVSDADGGGRRLYQEGTAGNLMTLTNLRAALEA
                     DTGADPRACLLLSGTISMAGDMLCAARFEMELVDPATGAALRHAYTVEPF"
     gene            complement(1517..2299)
                     /locus_tag="VF357_03325"
     CDS             complement(1517..2299)
                     /locus_tag="VF357_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7523208.1"
                     /translation="MRKTRIGGLILLVLLWAGHAFAWSFAVCGDSRDDRDGIFPQILS
                     AVDTSDMEFLLHTGDMVNKDSPGEWNLYRDATARFRKPLYVVIGNHEFYGGGTPEKFA
                     ERFGLPGASWSFTHKDAHFAIVDNAKGTFPDNTLAWLDRDLAAHPKGKDGITTLIVAM
                     HIPPGPGGIRPHGTRYDYEERSAKLLAILKRHGVAAILSGHEHMQYVEDWEGVLLLIS
                     GGAGAPLAPFQRYGYYRIDVENGRVRETFQPVKPIKKSSLPN"
     gene            complement(2314..3546)
                     /locus_tag="VF357_03330"
     CDS             complement(2314..3546)
                     /locus_tag="VF357_03330"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7523209.1"
                     /translation="MVPPGNGAVASTFRGLRHRNFRLWFFGQLTSLVGTWMQTIAQNW
                     LVYQLTGSARDLGLVNFVGAIPLVPLTLYAGAIADRFEKRKVIFWCQAAMMALAFLLA
                     ALCWTGTVRFWHVLLLAFLLGAVQALDTPARQAFVVDLVGKEDLSNAIALNSGAFHAA
                     RVLGPAAAGLLITFSGVAGAFFINGASFLAVLFGLFLMDVALIRSTGAGDESAKDLLG
                     GVRYLRKERLPRAVVVLISLSALFAMPYMVLIPIYAREIFGRGADGYGVLMSAAGVGA
                     VLGSLYSASPRVGAHKGAAITAGSLTFPFLLLAFAFCRSYHASILLLVGVGYTFVLQN
                     APANSLLQELVPDHLRGRVMAIYVSLFLGLLRVGSLLLGGLAAVTSAPTALAALAAAG
                     VLVGLWVRFRYPELHSAP"
     gene            complement(3547..>5307)
                     /gene="glgB"
                     /locus_tag="VF357_03335"
     CDS             complement(3547..>5307)
                     /gene="glgB"
                     /locus_tag="VF357_03335"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX7523210.1"
                     /translation="AERVSVVCNFNHWDGRVHPMRNRGESGIWEIFLPGVVEEEVYKY
                     EIRSRGTGELLAKTDPFGFRFELPPRTGTIVCALEGHEWGDASWLESRARRNILEAPM
                     AVYEVHLGSWKRKEGEGGPYLSYRELADDLIPYVKEMGFTHIELLPVAEHPFDGSWGY
                     QVLGYFAPTSRHGRPAEFMEFVDRCHREGIGVIVDWVPAHFPRDAHGLARFDGTHLYE
                     HADPRRGEQKDWGTLIFNYGRREVANFLLSNALFWMDKYHIDGLRVDAVASMLYLDYS
                     RKPGQWVPNVYGGNENLEAIAFLKRMNELVYERHPGAITVAEESTAWPAVSRPTYLGG
                     LGFTHKWNMGWMHDMLSFIEQEPIHRKFHFGQLTFALLYAFHENFVLPFSHDEVVHLK
                     RSMLDKMPGDLWGKFANLRLLYAYMYAHPGKKLLFMGEEFAQWEEWNHDRSLSWELLR
                     WDTHQGVQRFVRDLNRLYREVPALHEVDFRPEGFEWIDFRDVDNSVVSFLRRGKNPDD
                     VVVCVFNFTPVPREKYRVGVPWDGRYREALNSDAAAYGGSNAGNGGTVAAESIPWMGH
                     PHSVSLTLPPLGALFLLRER"
BASE COUNT          940 a         1677 c         1779 g          911 t
ORIGIN      
        1 tcgacttcct cttctacgcc gtcatcgtcg acttctccct cgaactcgtc gacttcatcc
       61 accgggtgta ccagagcgag gaggagatca agatcctctc cgagatggtg atgagcaagc
      121 tgttcgggag cctcatcatc atccaggtcc tgctggggat gctggttccc ctgctgctca
      181 tctccctcac caagatcttc aagaagcgct tccacctgaa cgaggacctg cgaaagatgg
      241 tctacttcgt cgccgtgatc ctcatccagt tcgggatctt cgccacgcgg tggaacgtcg
      301 tcatcggggg ccagatgttc tcgaagagct tccgcgggct caccacgtac aagatggggt
      361 tcggggggct cgaagggctt ctttacgcga tcgttctgct tgccctgccg atcgtcatcc
      421 tggccgttct ggtgaaggtc ctgccgccgt ggaaagagat ggacggcgaa ccggcctccg
      481 aaggggtgga ggtcgcgggc tcctgagccg gctgccgcag ggccggcctc ttttctcgag
      541 acgtgaatag agcgggagtt tcttcggtat aggcatccct tgctgcagcg gttttgttga
      601 tatataagat gcatatctta tggatatcaa tcttgccgac cacaagggaa tgtctggagc
      661 cgatacgcgc cgacagtccc aatccctccg aaaccaggcc ctgtccctac tgcgtcaggc
      721 ggtagtgcac ttgcagtatg ctcctggcgc ggtagtgagc gaagcggagc tggtggtaca
      781 ggtcgggctc ggcaggaccc ccgttcgcga ggcggtggcg cgtctggaac aggaaggtct
      841 cgtgaccgtc cttccgcgca aggggattca gatcacgccg gtaaccttgg tggacctgca
      901 ggcactttgc gagctgcgca tggaactgga aggatggggg cacggctggc cgcggagcgt
      961 cgtaccccgg agcaactgag ggtgatgcag gggctgtttg cggaggcgcc ggtgctgatt
     1021 gccgccaacg acctgtcgca gttgctggaa ctggaccggg atttccacat gctgttggcc
     1081 cgttgcacca agaacacata tctggaagag acgctctccc gcctctacaa caacagtttg
     1141 cgctattgct ttgccccaaa gtgctcgcgc cgcatgcgtg gccggtgcgg caggtgatgg
     1201 atcggtggga tactttgcag ttgcgcagct atgtgagcga tgccgatggc ggtgggcgcc
     1261 gcctgtacca ggaggggacc gccggcaacc tcatgacgct tacgaacctg cgcgcggcgt
     1321 tggaggcgga caccggcgcc gatccccggg cctgcctgct cctgtcggga acgatatcga
     1381 tggcggggga tatgctgtgc gctgcgcgct ttgagatgga attggtcgat cctgccaccg
     1441 gtgccgcttt gcggcatgcc tacaccgtgg aaccatttta aagggtgccg gaaacatacc
     1501 ggttgcacga ggagcgttag ttcggcaaag agcttttctt gatgggcttg acgggctgga
     1561 acgtctccct cacccttccg ttctcgacgt cgatccggta gtagccgtac ctctggaacg
     1621 gcgcgagtgg cgcgccggcg cccccggaga tcagcagcag gaccccttcc caatcctcca
     1681 catattgcat gtgctcgtga ccgctcagga tggcggccac gccgtgccgc ttcaggatcg
     1741 cgagcagctt cgcgctccgc tcctcgtagt cgtaccgggt cccgtggggc cggatccctc
     1801 ccggacccgg ggggatgtgc atcgccacga tgagggtcgt gatcccgtcc tttcccttcg
     1861 ggtgggcggc gaggtcgcgg tcgagccacg cgagggtatt gtccgggaac gtccccttcg
     1921 cgttgtccac gatcgcgaag tgggcgtcct tgtgggtgaa ggaccaggag gcgcccggga
     1981 ggccgaacct ctccgcgaac ttctccggtg tcccgcctcc atagaactcg tggttcccga
     2041 tcacaacgta cagcggtttt cggaaccgtg ccgtcgcatc gcgatacagg ttccactcac
     2101 cgggcgagtc tttgttgacc atgtcccccg tgtgaagcag aaactccata tcggaggtgt
     2161 ccacggcgga gaggatctgc ggaaagatcc cgtcccgatc gtcccggctg tcgccgcaca
     2221 ccgcgaacga ccatgcgaag gcatggcccg cccaaagtag gacgagcagg atcaggccgc
     2281 cgatccgtgt ctttcgcatg cggcgattat acctcacggg gcgctgtgga gctccgggta
     2341 ccggaagcgg acccacaagc cgacgagcac gcccgcggcg gcgagggcgg cgagggcggt
     2401 gggggcggag gtcaccgcgg ccaacccccc gaggagaagg ctcccgaccc ggaggaggcc
     2461 gaggaatagg gagacgtaga tcgccatcac ccgcccgcgc agatgatccg gcaccagctc
     2521 ctgcaagagg gaattcgcgg gggcgttctg cagcacgaag gtgtacccga ccccgaccag
     2581 gagcaggatg gaggcgtggt agctccggca gaaggcgaac gccagcagca ggaagggaaa
     2641 cgtcaggcta cccgccgtga tggccgcccc cttgtgcgcg ccgacgcggg gcgacgcgga
     2701 gtagagcgac ccgaggaccg cgccgacccc cgcggcggac atgagaaccc cgtacccatc
     2761 ggccccgcgt ccgaagatct cccgcgcgta gatggggatc agcaccatgt acggcatggc
     2821 aaaaagggcc gacagggaga tgaggacgac gacggcccgc ggcaggcgct ccttccggag
     2881 gtaccgcacc cctccgagaa ggtccttcgc ggattcgtcc ccggcgccgg tgctgcggat
     2941 gagcgccacg tccatcagga acaggccgaa gaggacggcg aggaaactcg ccccgttgat
     3001 gaagaaggcg cccgcgacgc ctgagaaggt gatgagcagc ccggccgcgg cggggccgag
     3061 gacgcgcgcg gcgtgaaatg ccccggagtt gagggcgatg gcgttcgaca ggtcctcctt
     3121 ccccaccaga tcgacgacga aggcttgccg ggcgggggtg tccagcgcct gcacggcccc
     3181 gaggaggaac gcgaggagga ggacgtgcca gaagcgcacc gttcccgtcc agcagagggc
     3241 ggcgagcagg aacgcgaggg ccatcatcgc ggcctggcac cagaagatca ccttgcgctt
     3301 ctcgaagcgg tccgcgatcg caccggcgta gagggtgaga ggaacgaggg ggatcgcccc
     3361 gacgaagttc acgagcccga ggtcccgcgc ggagccggtg agctggtaca cgagccagtt
     3421 ctgggcgatc gtctgcatcc aggtgcccac gagggaagtg agctgtccga agaaccagag
     3481 gcggaagttg cggtggcgca gcccccggaa cgtcgaggcg accgcgccgt tcccgggcgg
     3541 gaccacttat ctctcgcgca ggaggaagag cgcgcccagt gggggaagcg tgagggagac
     3601 ggagtgggga tgccccatcc acggtatgct ctccgccgcg acggtgccgc cgttcccggc
     3661 gttgctcccg ccgtaggcgg cggcgtcgct gttcagcgcc tcgcggtacc gtccgtccca
     3721 ggggacgccg acgcggtact tctcgcgagg aaccggagtg aagttgaaga cgcacaccac
     3781 gacgtcgtcg gggttctttc cccgacgcag gaacgagacg acgctgttgt ccacgtcgcg
     3841 gaagtcgatc cactcgaacc cctcggggcg gaaatcgacc tcgtggagcg cggggacttc
     3901 ccggtagagg cggttcaggt cacggacgaa ccgctggacc ccctggtggg tgtcccaccg
     3961 cagcagctcc cacgagagcg accggtcgtg gttccactcc tcccactggg cgaactcctc
     4021 ccccatgaac aggagcttct ttcccgggtg ggcgtacatg taggcgtaga ggaggcggag
     4081 gttcgcgaac ttcccccaca ggtcgcccgg catcttgtcg agcatcgagc gcttcaggtg
     4141 aaccacctcg tcgtgagaga aggggaggac gaagttctcg tggaaggcgt acaggagggc
     4201 gaaggtgagc tggccgaagt ggaacttccg gtggatcggt tcctgctcaa tgaaggagag
     4261 catgtcgtgc atccagccca tgttccactt gtgggtgaac cccaggccac cgaggtacgt
     4321 cggccgcgac acggccggcc aggcggtcga ctcctccgcc accgtgatcg cgccggggtg
     4381 ccgctcgtat accagttcgt tcatccgctt gagaaaggcg atcgcctcga ggttctcgtt
     4441 ccctccgtaa acgttcggga cccactggcc cggtttccgg gagtagtcga ggtagagcat
     4501 cgacgccacg gcgtccaccc gcaggccgtc gatgtggtac ttgtccatcc agaagagggc
     4561 gttcgacagg aggaagttcg ccacctcccg gcgcccatag ttgaagatga gtgttcccca
     4621 gtccttctgc tcccctcgcc gcgggtcggc gtgctcgtag aggtgggtcc cgtcgaaccg
     4681 cgccagcccg tgtgcgtcgc ggggaaagtg ggccggcacc cagtcgacga tcactccgat
     4741 cccttcccgg tggcaccggt cgacgaactc catgaactcc gccgggcggc cgtggcgcga
     4801 cgtcggtgcg aagtacccga gcacctggta tccccacgac ccgtcgaacg gatgctccgc
     4861 caccgggagg agctcgatgt gggtgaatcc catctccttg acgtagggaa tgagatcgtc
     4921 ggccagttcc cggtacgaca ggtacggccc cccctcgccc tccttccgtt tccaggagcc
     4981 aaggtggacc tcgtacaccg ccatgggcgc ctcgaggatg tttcgtcgcg cgcggctctc
     5041 cagccaggac gcgtcccccc actcgtgccc ttcgagggcg cagacgatcg tccccgtccg
     5101 cggcgggagc tcgaaccgga agccgaacgg gtcggtcttg gcgagcagtt cgcccgtgcc
     5161 cctggagcgg atctcgtact tgtagacctc ctcctcgacg acgccgggga ggaagatctc
     5221 ccagatcccg gactctcccc ggttgcgcat cgggtgcacg cggccgtccc aatggttgaa
     5281 attgcagacg acggagacgc gctccgc
//
LOCUS       DASVJL010000166         7641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_682177, whole genome shotgun sequence.
ACCESSION   DASVJL010000166 DASVJL010000000
VERSION     DASVJL010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682177"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VF357_03340"
     CDS             <1..666
                     /locus_tag="VF357_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7523211.1"
                     /translation="PTTQVVDLDFFKRVVAFAKEHKMLVIHDLAYADLVFDGYKAPSI
                     LQVPGAKDVAVEMYSMSKGYSMPGWRVGFVVGNKRMVGALTRIKSYLDYGMFQAIQVA
                     ATVALNGPHRVVDEAVEIYRKRRDCLVEGFARIGWEFEKPRGTMFVWAPIPEPFREMG
                     SVEFSKLLLTKAKVAVSPGIGFGEHGEGFVRFALVENEHRIRQAIRGVKGMLQSPPKV
                     KSK"
     gene            663..1982
                     /locus_tag="VF357_03345"
     CDS             663..1982
                     /locus_tag="VF357_03345"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX7523212.1"
                     /translation="MTVSPREIGVGVVGFGTVGTGTVKLLLENAELLRKRVGIPIRLV
                     RVADRDVEKDRGVRLPEGVFIEDGMRVARDPDVHILVELVGGTGAAKDFLLEAIRNGK
                     SVVTANKALLAECGPEIVKAVQAAGVDIGFEASVGGGIPIIRTLREGLAANRIRAIFG
                     IINGTCNYILSRMTREGKPFAEVLADAQAAGLAEEDPSFDVDGIDTAHKLAILVWLAT
                     GGHVPPKEIFVQGIREIDQDDISFAKEFGYTIKLLAIAKENGAGIEARVHPTMIPSHS
                     PLATVDGSYNAIYVKGNFVGSSLSYGQGAGMLPTASAVVSDVIEIARNLRRGCAGRIP
                     PGGFFLADPSARADLAPFDQVHSEYYLKFRVVDKPGVLSRIAGVLGSHAISIASVLQK
                     GQGEVSVPIFIVTHRAKENDMRAALAEVDRLPDILDRTRMIRIENNL"
     gene            1999..3054
                     /gene="thrC"
                     /locus_tag="VF357_03350"
     CDS             1999..3054
                     /gene="thrC"
                     /locus_tag="VF357_03350"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX7523213.1"
                     /translation="MHWKGIIRHYGRFFTSVPEEAVVTLLEGNTPLIPAPALARRIAP
                     GTELYLKYEGLNPTGSFKDRGMTMAVSKAKADGSDSVICASTGNTSASAAAYAARAGM
                     KAFVLIPEGKIALGKLSQAMIHGAQVLQVLGNFDEALSLVKEISAKYPVTLVNSLNPY
                     RIEGQKSAAFEIVDALGDAPDYHILPVGNAGNITAYWAGYKEYRAAGKSKRCPVMLGW
                     QAEGAAPIVRGAPVAKPETVATAIRIGNPASWKQAEAARDESGGIIRMVSDAEILEAY
                     KMVAETEGVFCEPASAASIAGVIKLGTEGFFRAGQRLVCTLTGHGLKDPDNAIAQSVA
                     PVTIPPVLGDVLRILGF"
     gene            3066..4271
                     /locus_tag="VF357_03355"
     CDS             3066..4271
                     /locus_tag="VF357_03355"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR02535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HEX7523214.1"
                     /translation="MGGKYIILIGDGMADWPIPAIGNRTPLEAAEKPNMDFMAANGAV
                     GMVQVVPEDMYPGSDVSNLSILGYDPAVVYTGRSPLEAASIGISLGPDDVAVRCNLVA
                     LKNDGADSEMEDFSAGHISTSEAAELLASLQEAVADKGVRFHTGVSYRHLMVWPGGCD
                     GVKTTPPHDIHGKKITPYLPQGDGAEFLLTIMEISRELFSDHPVNRKRAAAGKLPGNA
                     VWLWGQGKAPRIPTFEEKFGLTGSVVAAVDLIKGIGIYAGLEVVSVPGATGYTDTNYG
                     GKAEYALRELERKDFVLIHVEAPDEAGHNGSVEEKIRAIERIDREMLTPLLARVREKG
                     DLRILLLPDHPTPVAIRTHAQEPVPFVFYPAPPGLSTTPAKRYTEADGRDSGQFLPAG
                     TRLIEYLLA"
     gene            4307..4702
                     /locus_tag="VF357_03360"
     CDS             4307..4702
                     /locus_tag="VF357_03360"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7523215.1"
                     /translation="MIFGDTDAAGIVYYGNYLRWFEVGRAELMRQKGFSYLVTMERGV
                     LLPVIEASALYHAPARYDDVLRIHAEIRGVRGVRLTFGYRIEREDGISLVTGHTVHAF
                     TGRDGKPVRPPAEFRALSLSNNISQGKGA"
     gene            4705..5661
                     /gene="glpX"
                     /locus_tag="VF357_03365"
     CDS             4705..5661
                     /gene="glpX"
                     /locus_tag="VF357_03365"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX7523216.1"
                     /translation="MERNLAMEVVRVTEAAALAAARLMGRGDNIAADQAAVTAMRKAL
                     SYVQFKGRVVIGEGERDEAPMLYIGEEVGASENPKVDIAVDPLEGTNIVSAGGYNAIA
                     VIAIAEEGGFLHAPDTYMQKLAVGPKARGVIDITATPTENLRRIAKAQKAYVEDLCVV
                     ILDRPRHQDLIREVREAGARIKLIGDGDVAGAIATSKEDSGVDVLMGTGGAPEGVLAA
                     AALKCMGGDFQGVLKFRNKEEIERAKVMGVTDLNRVYALNDLASSDVMFAATGVTFGD
                     FLKGVRFFSGGAFTQSVVMRSRSRTTRVIDTTHYFEFKPKYE"
     gene            5665..5889
                     /locus_tag="VF357_03370"
     CDS             5665..5889
                     /locus_tag="VF357_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7523217.1"
                     /translation="MEEIVTATGRIEIDFERCKACELCVPACPKGCIGMGTAINPQGY
                     GSAVFERPDDCTGCAICAETCPDACILVWR"
     gene            5886..6965
                     /locus_tag="VF357_03375"
     CDS             5886..6965
                     /locus_tag="VF357_03375"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HEX7523218.1"
                     /translation="MSALAPSTSKRILTKGNEAICLGAVAAGCRHYYGYPITPQNDIP
                     EYMAAHLPAIGGTFLQAESEVATINFILGTSASGKRVMTSSSGPGISLMQEGLSYMAG
                     SELPAVIVNISRSGPGLGGIAPSQGDYFQAVKGGGHGGYRLIVLAPHSVQEMYALTML
                     AFDLADKYRTPAMILGDAIVGQMKEPFVASPYDPASTVEKPWALTGCAGRERQNVKSL
                     HLKDNAIEVHNWKLHEKYERIRKDEARAESYMLDGATIAIVAFGTAARVSKTAIQWAR
                     NEGIAAGMLRPITLFPFPQQEVFELARTVDVLLVIEMNTGQMVEDVRQCTPFHDRIAF
                     LGKPCVMPTPEEILDRIRLLAGRKG"
     gene            6962..>7641
                     /locus_tag="VF357_03380"
     CDS             6962..>7641
                     /locus_tag="VF357_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7523219.1"
                     /translation="MSPPVKVGFTKAVYTRPRSLVDVKSHFCPGCHHGTIHKIVAECI
                     DRYGLRERTIGVACVGCAVFLYDYIDVDVVESPHGRAPAVATGVKRAQPDKFVFTYQG
                     DGDLAAIGTSEIIHAANRGENLSVIFVNNTTYGMTGGQMAPTTMLGQKTSTTPYGRDF
                     RSDGYPIRMAELLAGLEGTAYSARVAVSTPAQIRKAKESLRKAFEMQIGGMGFSIVEF
                     LSTCPTNWG"
BASE COUNT         1422 a         2402 c         2578 g         1239 t
ORIGIN      
        1 ccgacgaccc aggtggtgga cctcgatttc ttcaagcgcg tcgtcgcgtt cgccaaagag
       61 cacaagatgc tggtcatcca cgatctcgcc tacgcggacc tcgtcttcga cgggtacaag
      121 gcgccctcga tcctccaggt ccccggggcc aaggacgttg ccgtggagat gtattccatg
      181 tcgaagggat actccatgcc cggctggcgc gtcggcttcg tcgtcggaaa caaacggatg
      241 gtcggagcgc tgacccggat aaagagctac ctcgactacg gaatgttcca ggcgatccag
      301 gtcgccgcca cggtggcgct caacgggccg caccgcgtgg tggacgaggc cgtcgagatt
      361 tatcggaagc gcagggattg cctcgtcgag ggattcgccc gcatcgggtg ggagttcgag
      421 aagccgaggg gaacgatgtt cgtgtgggcg cccattcccg aaccgttccg cgaaatggga
      481 tccgtcgagt tttcgaagct tctcctgacg aaggccaagg tcgcggtttc gccgggaatc
      541 ggcttcgggg aacatgggga agggttcgtc cggttcgcgc tggtggagaa cgagcaccgg
      601 atacggcagg cgatccgcgg cgtaaagggg atgctccagt cgccgccgaa ggtcaagtcg
      661 aaatgaccgt ttccccccgg gagatcgggg tcggcgtcgt cggtttcggc accgtcggaa
      721 ccggcacggt caaacttctc ctcgagaacg cggaactcct ccgcaagcgc gtcgggatcc
      781 cgatccggct cgtccgggtc gcggaccggg acgtggagaa ggatcggggc gttcgactgc
      841 ccgagggcgt cttcatcgag gacggcatgc gggtcgcccg ggaccccgat gtccatatcc
      901 tcgtcgaact tgtcggcggc acgggcgccg cgaaagactt cctgctcgag gcgatccgca
      961 acggaaaatc ggtggtgacg gcgaacaagg ccctgctcgc ggagtgcggt ccggagatcg
     1021 tcaaggccgt ccaggccgcc ggcgtcgaca tcgggttcga ggcgagcgtc ggcggaggga
     1081 tcccgatcat ccggacgctg cgggaaggtt tggccgcgaa ccggatccgg gcgatcttcg
     1141 ggatcatcaa cgggacgtgc aactacatcc tttcgcgcat gaccagggag gggaagccgt
     1201 tcgccgaggt cctcgccgat gcgcaggccg ccgggctcgc cgaggaagac ccgtcgttcg
     1261 acgtcgacgg gatcgacacg gcgcacaagt tggcgatcct cgtctggctc gccaccggcg
     1321 ggcacgtccc ccccaaggag attttcgtcc aggggatccg ggagatcgac caggacgaca
     1381 tctctttcgc gaaggagttc gggtacacga tcaagctgct ggcgatcgcg aaggagaacg
     1441 gcgcggggat cgaggcgcgg gtccatccga cgatgatccc gtcccattcc cccctggcca
     1501 ccgtcgacgg ctcctataac gcgatctacg tgaaggggaa cttcgtcggt tcctccctgt
     1561 cgtacgggca gggtgcgggg atgttaccga cggcgtcggc ggtggtgagc gacgtgatcg
     1621 agatcgcgcg gaatcttcgc cggggatgcg cgggccggat acctcccgga gggttcttcc
     1681 tcgccgatcc gtcggcccgg gcggacctgg cgccgttcga ccaggtccac agcgagtact
     1741 acctgaaatt ccgggtggtg gacaagcccg gcgttctctc tagaatcgcg ggggtgctgg
     1801 ggagccacgc gatcagcatc gcctccgttc tccagaaggg acagggggag gtctcggttc
     1861 ccattttcat cgtcacccac cgggcgaagg agaacgacat gcgggcggcc ctcgccgagg
     1921 tggaccgtct ccccgatatc ctcgaccgga cgcgaatgat ccggatcgaa aacaatctct
     1981 aaaaggaacc gaggcgaaat gcactggaaa gggatcatcc gccattacgg gcggttcttc
     2041 acctccgttc ccgaggaggc cgtcgtcacg ctgttggagg ggaacactcc cctcataccg
     2101 gcccccgcgc ttgcccgccg gatcgctccg ggaaccgagc tttacctcaa gtacgagggg
     2161 ctgaacccga ccggttcctt caaggaccgc gggatgacga tggccgtctc aaaggcgaag
     2221 gcggacggtt ccgattcggt catctgcgcg tctaccggaa acacgtccgc ctccgcggcc
     2281 gcctacgccg cacgtgccgg aatgaaggca ttcgtcctca tccccgaggg gaagatcgcg
     2341 ttggggaaac tctcccaggc gatgatccac ggggcgcagg tactgcaggt gctgggcaac
     2401 ttcgacgagg ccctttctct cgtcaaggaa atctcggcaa agtatcccgt gacgctggtg
     2461 aactcgctga acccgtacag gatcgagggg cagaagtcgg ccgctttcga gatcgtcgac
     2521 gccctcggag acgcgcccga ctaccacatc ctcccggtgg gaaacgcggg aaacatcacc
     2581 gcgtactggg ccggctacaa ggagtatcgg gccgccggga agtcgaagcg ctgtcccgtg
     2641 atgctcggct ggcaggcgga aggggcggcc ccgatcgtcc gtggagcccc tgtcgcgaag
     2701 cccgagacgg tcgccaccgc catccggatc ggcaaccccg catcgtggaa gcaggccgaa
     2761 gccgcgcgcg acgagtccgg ggggatcatc cgcatggtga gtgacgcgga gatcctggag
     2821 gcgtacaaga tggtcgccga gacggaaggg gtcttctgcg agcccgcgtc ggccgcctcg
     2881 atcgccggcg tgataaaatt ggggaccgaa ggatttttcc gcgcggggca gcgtctcgtt
     2941 tgcacgctga ccgggcacgg cctgaaggac ccggacaacg ccatcgcgca gtcggtcgcg
     3001 cccgtgacga tccccccggt actcggggac gtcctcagga tcctcggatt ctgaaggaga
     3061 cgacgatggg tgggaaatac atcatcctga tcggggacgg catggccgac tggccgattc
     3121 ctgcgatcgg caaccgcacg ccgctcgagg ccgcggagaa accgaacatg gacttcatgg
     3181 cggccaacgg cgccgtcggg atggtccagg tcgtaccgga agatatgtac ccgggcagcg
     3241 acgtgtcgaa cctgagcatc ctggggtacg accccgccgt cgtctacacg gggcgctctc
     3301 cgctcgaggc ggcctccatc ggcatctccc tcggaccgga cgacgtcgcg gtccgctgca
     3361 atctggtggc gctgaaaaac gacggggccg actccgagat ggaggatttc tccgccgggc
     3421 atatctccac ctccgaggcg gcggagctcc tcgcctccct ccaggaggct gtcgccgaca
     3481 agggggtccg gtttcacacg ggcgtgtcgt atcgccacct gatggtttgg cccggggggt
     3541 gcgacggcgt gaagacaacc cccccgcacg acatccatgg aaagaagatc accccctacc
     3601 tcccccaggg ggacggcgcg gaatttctcc tgacgatcat ggagatctcc cgcgaactct
     3661 tctccgacca cccggtgaac cggaagaggg cggcggcggg gaagcttccg ggcaacgcgg
     3721 tctggctctg ggggcagggg aaggcccccc gcatccccac gttcgaggaa aaattcggct
     3781 tgaccgggtc cgtggtcgcc gcggtggacc tcatcaaggg gatcgggatc tacgcgggcc
     3841 tcgaagtggt ctccgttccg ggagccaccg gatacaccga cacgaattac ggggggaagg
     3901 cggaatacgc ccttcgcgag ctcgagcgga aggacttcgt cctgatccac gtggaggccc
     3961 ccgacgaagc gggacacaac gggagcgtcg aagagaagat ccgcgcgatc gaacgaatcg
     4021 accgggagat gctcaccccg ctcctcgcga gggtgcggga gaaaggggat ctccggattc
     4081 tccttctgcc ggaccatccg acccccgtcg ccatccggac ccacgcgcag gagccggtcc
     4141 cgttcgtctt ctacccggcg cccccgggat tgtccacgac cccggcaaag cggtatacgg
     4201 aagcggacgg ccgcgattcc gggcagttcc tcccggcggg tacccgcctg atagaatacc
     4261 tgctcgcctg acgggagccc tcgtggagca tgtcgccacc gttagggtga tcttcggtga
     4321 tacggatgcc gccggcatcg tctattacgg aaattacctt cgctggttcg aagtcggccg
     4381 ggcggagctg atgcggcaaa aaggattttc gtacctcgtc acgatggaac ggggggtact
     4441 cctccccgtc atcgaggcga gcgctcttta ccacgcaccc gcgcggtacg acgacgtgct
     4501 tcggatccac gcggagatcc ggggcgtccg gggtgtgcgc ctgacgttcg ggtaccggat
     4561 cgagcgagaa gacgggattt ccctggtgac ggggcatacc gtgcacgctt tcacgggacg
     4621 ggacgggaag ccggtgcggc ctcccgcgga gttccgcgcg ctgtcgcttt cgaacaacat
     4681 ttcccaggga aagggggcct gacgatggag cggaacctgg caatggaggt cgtccgtgta
     4741 accgaggcgg cggcgctcgc cgcggcgcgg ttgatgggcc gcggggacaa catcgctgcg
     4801 gaccaggctg cggtcaccgc gatgcggaaa gcgctgagct acgtccagtt caaggggcgc
     4861 gtcgtcatcg gggaagggga gcgggacgaa gcccccatgc tctacatcgg ggaggaggtc
     4921 ggcgcgtcgg aaaatccgaa ggtcgacatc gccgtcgatc ctctcgaggg gacgaacatc
     4981 gtgtcggccg gcgggtacaa cgccatcgcc gtgatcgcca tcgcggaaga gggcgggttt
     5041 cttcatgccc ccgacacata catgcagaag ctcgcggtgg gaccgaaggc acgaggcgtg
     5101 atcgacatca cggcgacccc gacggagaac ctccgcagga tcgccaaggc gcagaaggcc
     5161 tacgtcgagg acctttgcgt ggtgatcctc gaccggccgc ggcaccagga tctgatcaga
     5221 gaggtccggg aagccggggc ccggatcaag ctgatcgggg acggcgacgt ggcgggcgcg
     5281 atcgctacat cgaaagagga ttccggggta gacgtcctca tggggacggg cggtgcgccg
     5341 gaaggggtcc tggccgccgc ggcgctgaaa tgcatgggcg gcgatttcca gggcgtcctc
     5401 aagttccgga acaaggaaga aatcgagcgc gccaaggtga tgggggtcac cgacctgaac
     5461 cgcgtctacg ccctcaacga cctggcgagc agcgacgtga tgttcgccgc caccggggtc
     5521 accttcggcg atttcctgaa aggggtccgg ttcttcagcg gcggcgcgtt cacccagtcc
     5581 gtcgtcatgc gctcccgctc ccgcaccaca cgcgtcatcg acacgaccca ctacttcgaa
     5641 ttcaaaccga agtacgaata ggcattggag gagatcgtta cggcgacggg gcggatcgag
     5701 atcgatttcg agcggtgcaa ggcgtgcgaa ctttgcgttc ccgcttgccc gaaggggtgc
     5761 atcgggatgg gcacggccat caacccgcag gggtacgggt ccgccgtctt cgagcggcct
     5821 gacgactgca cggggtgcgc gatctgcgcc gagacgtgcc ccgacgcgtg catcctggtg
     5881 tggcgatgag cgcgctggcg ccgtccacgt cgaagcgaat cctcaccaag gggaacgagg
     5941 cgatctgcct cggggccgtc gcagccgggt gccgtcacta ttacggctac ccgatcactc
     6001 cacaaaacga catccccgaa tacatggcgg cgcacctgcc ggcaatcggc ggaacgtttc
     6061 tccaggcgga gagcgaagtg gcgacgatca acttcatcct cggaacctcc gcctccggga
     6121 agcgggtgat gacgtcctcc tcgggccccg ggatctccct catgcaggaa ggactctcct
     6181 acatggcggg atccgaacta cccgcggtga tcgtcaacat ttccaggtcg ggtccgggcc
     6241 tcggcggcat cgccccctcc cagggggatt acttccaggc ggtcaagggc ggcggccacg
     6301 gggggtaccg gctgatcgtc ctggcgccgc actcggtcca ggagatgtat gccctcacca
     6361 tgctcgcctt cgaccttgcg gacaaatatc gcactcctgc catgatcctc ggagacgcca
     6421 tcgtcggcca gatgaaggaa cccttcgtcg cttctccgta cgatccggcg tcgacggtgg
     6481 agaaaccgtg ggcgctgacc ggttgcgcgg gacgcgagcg acaaaacgtg aaatcccttc
     6541 acctgaaaga caacgccatc gaggtacaca actggaagct gcacgaaaag tacgagcgga
     6601 tccgcaagga cgaggcgagg gcggagtcgt acatgctcga cggcgccacg atcgcgatcg
     6661 tcgcgttcgg gaccgcggcc cgcgtgagca aaaccgccat ccagtgggcc cgaaacgagg
     6721 ggatcgccgc cgggatgctg cgtccgatca cgctcttccc gtttccacag caggaggtgt
     6781 tcgaactggc gaggacggtc gacgtcctgc tcgtcatcga aatgaacacc gggcagatgg
     6841 tggaggacgt gcgtcaatgc acgccgtttc acgatcgaat cgcgtttctc gggaagccgt
     6901 gcgtcatgcc cacccccgag gagatcctcg accgcatacg actgctggca gggaggaagg
     6961 gatgagcccc cccgtgaagg tcgggttcac gaaagcggtt tacacgcggc cccggagcct
     7021 ggtcgacgtc aagagccact tctgtccggg ctgccaccac gggacgatcc acaagatcgt
     7081 cgcggagtgc atcgaccggt acggtctccg ggagaggacg atcggagtcg cttgcgtcgg
     7141 gtgcgccgtt ttcctgtacg actacatcga cgtggacgtg gtcgagtcgc cgcacgggcg
     7201 cgccccggcg gtggcgaccg gcgtcaagcg ggcgcagccg gacaagttcg tcttcaccta
     7261 ccagggggac ggtgacctgg cggcgatcgg aacgtcggag atcattcacg cagcgaaccg
     7321 cggggagaac ctctccgtga tcttcgtgaa caacacgaca tacggaatga ccgggggtca
     7381 gatggcgccc accaccatgc tcgggcagaa gacctccacc acaccgtacg ggcgcgattt
     7441 ccgaagcgac ggctatccga tcaggatggc cgaactcttg gccggcctcg agggaacggc
     7501 ctattccgcc cgggtcgcgg tctccacccc cgcccagatc cggaaggcga aggagtccct
     7561 ccggaaggcg ttcgagatgc agatcggcgg gatggggttc tcgatcgtcg agttcctctc
     7621 cacctgcccc acgaactggg g
//
LOCUS       DASVJL010000167         4350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_588418, whole genome shotgun sequence.
ACCESSION   DASVJL010000167 DASVJL010000000
VERSION     DASVJL010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588418"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1200
                     /locus_tag="VF357_03385"
     CDS             172..1200
                     /locus_tag="VF357_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7523220.1"
                     /translation="MFDRLLGLFSHDLAIDLGTATTLVYVKGEGIICSEPSAVAVHRD
                     ARGTKKVLAVGIEAKRMIGRTPGNIVAIRPIKDGVIADFEVTEAMLRYFIRKAHKART
                     LVRPRIIICVPYGCTEVERRAVRESAQSAGAREVYLIEEPLAAAIGAGLPITEPSGSM
                     IVDIGGGTTEVAVISLGGIVKSQSVRVAGDKMDEAILQYVKRKYNLLIGEPTAEHIKV
                     AIGNAFPGFSESTEIKGLDMVSGVPKALTLHSDEVREALSEPVRIIVDAVKSVFEETP
                     PELAGDIYDRGIVLAGGGALLRNLDALLREETGLPVTVAEDPLSCVVLGSGKALDELD
                     LLRQVALH"
     gene            1249..2094
                     /gene="mreC"
                     /locus_tag="VF357_03390"
     CDS             1249..2094
                     /gene="mreC"
                     /locus_tag="VF357_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX7523221.1"
                     /translation="MRSFFRNWWRLLVAVALLVAAIQVFVHPSAALERAEPVRAAGVV
                     LFRPFYSAADFLRGGLSGVWNRYVALVGVSRENDRLRKEVTDLREQLHATRDAVLENR
                     RLKELLGYSQTVERRTLGARVVGHDVSPWFRAIFLGTGSEAGIETGMSVVTPYGAIGR
                     IHKVHPGLSEVLLVTDGRFAADVMVERSRVRAIAEGVGGSFCRLKYVSPAQDVAVGDR
                     IVFSGFDGSMPKGVLLGTVVSVDRPKESLFQKVKVQCAVNFEDVEEVMVILSRPSVPF
                     RAVKD"
     gene            2091..2576
                     /locus_tag="VF357_03395"
     CDS             2091..2576
                     /locus_tag="VF357_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523222.1"
                     /translation="MKHFLVLLALSYLGASLCVWWLSGFVPWFLLPDFPFLAVVFSGL
                     FVPGIAGFLCALPPALFREITASAPSWSMFLGSMALYFASREVGRRFLLRSEPSLLAA
                     VVSLLAAESFSVVLLLSLSGARPFSFLWGAQEAVRIAWTSLIAVPLFLDLSVRWRRVK
                     E"
     gene            2573..>4350
                     /gene="mrdA"
                     /locus_tag="VF357_03400"
     CDS             2573..>4350
                     /gene="mrdA"
                     /locus_tag="VF357_03400"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX7523223.1"
                     /translation="MTGRIRKREQDPDITRRSRLLLYVAFGAFALLLGRLYWLQVVES
                     GRYRNLSENNRLRLRTVRAPRGLILDRKGRAIAETQGSFDLVCTPVDVKDLEAEIGLL
                     AEIVEFDVDDNEVRGKIRSAKRSNPYSSITVARDLRFEQVSVIEFNRESLPGFSVLVE
                     AKRSYPYGTAFAHVLGYVGEASPEEMEKSEDDSLAMGDLVGKYGLERLMDDDLRGVNG
                     GRKVEVDAAGRDQRLVEEVPSQAGGAVHTFLDADLQVTAQEALGTRAGAVIALAPKTG
                     EVLAFYSGPAFDPNVFARGIHKADWQALSADPRKPMQNKGLQGTYAPGSTIKPFLALA
                     ALEEKVQEKGKPVLCPGSYRLGNRVFRCWKEKGHGAVDMYRAIVQSCDVYFYTLGLKL
                     GPDRLAKLEKDAGLGTITGIDLPGERKGLVPDTAWKRTVSKDRWQGYESVILGIGQGA
                     VHLTPLEMAVGYATLATGGEVMRPRVVSKVIGKDGKVRAHAPEMLRKLPWNPENVEFI
                     RKALAGVVNDYGTGGGVKLPGIVVGGKTGTAQVVSVKGKMIKSEDLPYEIRDHAWFIA
                     FAPVDDPQIVVAAMVEHGGHGASAAAP"
BASE COUNT          669 a         1377 c         1543 g          761 t
ORIGIN      
        1 tcgatggaaa tgatagtgtt gttgaacgtg ccattctaat gccgcgcgag gtccgatatc
       61 aacgggattc cggcgggttc cccttcggat tgcaatgagg gtcgggggac tgctaagata
      121 ccttgatatg tttccgtgga catcgaacat gcgtctggag gatgcgggat gattttcgac
      181 cggctgctcg ggctgttttc acatgatctg gcgatcgacc tcggcaccgc gacgacgctg
      241 gtgtacgtga agggggaggg gatcatctgc tccgagccct cggccgtggc ggtccaccgg
      301 gacgcccggg ggaccaagaa ggtgctcgcc gtcgggatcg aggcgaagcg gatgatcggc
      361 cgcacgccgg ggaacatcgt ggcgatccgc cccatcaagg acggcgtcat cgccgatttc
      421 gaggtcaccg aggcgatgct ccgatacttc atccggaagg cccacaaagc ccgtaccctc
      481 gtgcggcccc gcatcatcat ctgcgtcccc tacggctgca ccgaggtgga gcggcgcgcc
      541 gtccgtgagt ccgcgcagag cgcgggcgcc cgggaggtgt atctcatcga ggagccgctg
      601 gcggcagcga tcggggcggg gctccccatc accgaaccgt ccggcagcat gatcgtcgac
      661 atcggcggag ggacgaccga ggtggcggtc atctccctcg gggggatcgt caagagccag
      721 tcggtgcggg tggccggcga caagatggac gaggcgatcc tccagtacgt gaagcggaag
      781 tacaacctgc tgatcggcga gccgacggcg gaacatatca aggtggcgat cgggaacgcg
      841 ttccctggct tctcggaatc cacggagatc aagggactcg acatggtctc cggggtgccc
      901 aaggcgctca ccctccactc ggacgaggtg cgcgaggcgc tgtccgaacc ggtccggatc
      961 atcgtcgacg ccgtcaagag cgttttcgag gaaaccccgc cggaactggc gggagacatc
     1021 tacgaccgcg gaatcgtgct cgccggcggc ggggcgctgc tgcgcaacct cgacgccctt
     1081 ctgcgggaag aaacagggct ccccgtcacg gtggcggagg accccctctc ctgcgtggtg
     1141 ctcgggtccg gcaaggcgct cgacgagctc gacctgctcc ggcaggtggc cctgcattaa
     1201 ccgatcgggg cggaccaagg tccgctccaa ggtctccaag cggtatctat gcgatccttc
     1261 ttccggaatt ggtggcggct cctcgttgcc gtggcgctcc tggttgcggc gatccaggtc
     1321 ttcgtccacc cttcggccgc ccttgagcgc gccgagccgg tgcgcgccgc cggtgtcgtc
     1381 ctcttccgtc ccttctactc cgccgcggac ttcctgcgcg gcggcctttc cggcgtgtgg
     1441 aaccggtacg tcgccctggt gggcgtgtcc agggagaacg atcggcttcg gaaggaggtg
     1501 acggatctcc gggagcagct ccacgcgacc cgggacgccg tcctcgagaa ccgtcggttg
     1561 aaggagctct tgggctactc gcagaccgtc gaacggcgga ccctcggggc gcgcgtggtc
     1621 gggcacgacg tctctccctg gttccgggcg attttcctcg gcacgggctc ggaggcgggg
     1681 atcgagaccg ggatgtccgt ggtcacaccc tacggcgcga tcgggcggat ccacaaggtc
     1741 cacccggggc tttccgaggt gctgctcgtc acggacggca ggtttgcggc cgacgtgatg
     1801 gtcgagcgca gccgggtccg cgccatcgcg gaaggggtgg gcgggagctt ctgccggctg
     1861 aagtacgttt cgccggcgca ggacgtcgcg gtgggggacc ggatcgtctt ctcggggttc
     1921 gatgggagca tgcccaaagg ggttctcctc ggcaccgttg tcagcgtcga ccgtccgaag
     1981 gagagcctct tccagaaggt gaaggtccag tgcgcggtga acttcgagga cgtcgaagag
     2041 gtgatggtga tcctctcccg cccctccgtg ccgttccggg cggtaaaaga ttgaaacatt
     2101 ttctcgtgct gttggctctg tcgtatctgg gcgcctccct ttgcgtctgg tggctctccg
     2161 ggttcgtccc ttggttcctg ctccccgact tcccgtttct cgcggtcgtt ttttcggggc
     2221 tcttcgttcc ggggatcgcg ggattcctgt gcgctcttcc ccccgcgctc ttccgcgaga
     2281 tcacggcgtc cgcgccttcc tggtcgatgt tcctcggatc gatggccctc tacttcgcct
     2341 cgcgggaggt cggccgccga ttcctcctgc gtagcgaacc gtcgctcctc gctgccgtcg
     2401 tgtccctgct ggcggccgag tcgttctccg tcgtccttct cctttccctt tccggggccc
     2461 gccccttctc cttcctgtgg ggggcgcagg aggccgtccg tatcgcctgg acctccctga
     2521 tcgccgtccc cctcttcctg gacctgtcgg tccgctggcg aagggtgaag gaatgaccgg
     2581 ccggatccgc aagcgggagc aggaccccga catcacccgc cgatcgcgtc ttctcctcta
     2641 tgtggccttc ggggcgttcg cgctcctcct cggtcggctc tactggctcc aggtggtgga
     2701 gtccggccgg taccggaatc tctcggagaa caaccgcctc cgcctccgaa ccgtccgcgc
     2761 gccgcgggga ctgatcctcg accggaaggg gagggcgatc gcggagacgc agggctcctt
     2821 cgatctcgtc tgcaccccgg tggacgtcaa ggacctcgag gcggagatcg gccttctcgc
     2881 cgagatcgtg gagttcgacg tcgacgacaa cgaggtgcgc ggcaagatcc ggtcggcgaa
     2941 gcggtcgaac ccctacagca gcatcaccgt ggcgcgggac ctccggttcg agcaggtctc
     3001 ggtcatcgag ttcaaccggg agagcctccc cggcttctcc gtcctggtcg aggcgaaacg
     3061 aagctacccc tacggaacgg ccttcgcgca cgtcctcggg tacgtgggcg aggcgagtcc
     3121 ggaggagatg gaaaagtcgg aagacgactc gctggcgatg ggggacctgg tcggcaagta
     3181 cggtctggag aggctgatgg acgacgacct gcgcggggtg aacggcggtc ggaaggtgga
     3241 ggtggacgcg gccgggagag accagcggct cgtcgaggag gtgccttccc aggccggcgg
     3301 cgccgtgcac accttcctgg acgcggacct ccaggtgacg gcccaggagg cgcttggaac
     3361 ccgggcgggg gccgtgatcg ccctcgcgcc gaaaaccggg gaggtcctcg cgttttattc
     3421 cggccccgcg ttcgacccga acgtgttcgc gcgggggatc cacaaggccg actggcaggc
     3481 gctgagcgcc gacccgcgca agccgatgca gaacaagggc ctgcagggga cgtacgcccc
     3541 gggctccacc atcaagccgt tcctcgcgtt ggcggccctg gaggagaagg tgcaggagaa
     3601 ggggaagccg gtcctctgcc ccgggtcgta ccgtctcggg aaccgggtct tccgctgttg
     3661 gaaagagaag gggcacggcg ccgtcgacat gtaccgggcg atcgtgcagt cgtgcgacgt
     3721 ctacttctac acgctcgggc tgaagctggg ccccgaccgt ctggcgaagc tggagaagga
     3781 cgcggggctg gggacgatca cggggatcga cctccccggg gagcggaagg ggctggtccc
     3841 ggacacggcg tggaagcgca ccgtgagcaa ggaccggtgg cagggctatg agagcgtgat
     3901 cctcgggatc ggccaggggg cggtccacct cactcccctc gagatggccg tcggctacgc
     3961 gacgctggcg acgggcggcg aagtgatgcg tccccgcgtc gtgtcgaagg tgatcgggaa
     4021 ggacgggaag gtacgggcgc acgcccccga gatgctccgg aagctcccat ggaacccgga
     4081 gaacgtggag ttcatccgaa aggcgctggc cggcgtggta aacgactacg gcacgggcgg
     4141 gggtgtgaag ctcccgggga tcgtggtggg cgggaagacg ggcaccgcgc aggtcgtctc
     4201 ggtgaagggg aagatgatca agtccgagga tcttccgtac gagatccgcg accacgcgtg
     4261 gttcatcgcg ttcgccccgg tggacgaccc gcagatcgtc gtggcggcga tggtggagca
     4321 cggcgggcac ggcgcatccg ccgccgcgcc
//
LOCUS       DASVJL010000168         8088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_963999, whole genome shotgun sequence.
ACCESSION   DASVJL010000168 DASVJL010000000
VERSION     DASVJL010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963999"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VF357_03405"
     CDS             complement(<1..390)
                     /locus_tag="VF357_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523224.1"
                     /translation="MEVIDPPESFLRWINDFRWDEEERFSFRSTIRATLRLPRLKKRW
                     KLVFSGETRGDPNAITPEDPGNPGLDVGSRVRTGSTELVYDILRTTPSTLDVGGRGPG
                     EDPTRRLRPHAAPARAARRVPYPRSVHG"
     gene            complement(595..1032)
                     /locus_tag="VF357_03410"
     CDS             complement(595..1032)
                     /locus_tag="VF357_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523225.1"
                     /translation="MNRFGKGVALCVSIGALAFGNAASGGNEGAKAPATSEQIKVEKA
                     KGADAVNVAETYEKAEKLDKKTVVVRGKVVKVSKGIMGKNWVHLRDGSGDPGKGTNNL
                     VVTTQDVPKVGDVVTAKGILSKDKDFGAGYKYKVIVEEATVKQ"
     gene            1203..1790
                     /locus_tag="VF357_03415"
     CDS             1203..1790
                     /locus_tag="VF357_03415"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX7523226.1"
                     /translation="MRVVAFNGSARKGGNTTLLLRHVFAELEKGGIDTELVELAGKRI
                     HGCTACYKCFANKDRRCAVKGDAVNDCIEKIDGADGILLGSPTYFTDVTAEMKALIDR
                     AGMVGRANGNMYRRKAGAAVVAVRRTGAIHAFDTMNHFFLIGEMIIPGSSYWNIGVGR
                     EIGDVEKDEEGVQTMRTLGENMAWLMKALAAAQRG"
     gene            1906..4563
                     /locus_tag="VF357_03420"
     CDS             1906..4563
                     /locus_tag="VF357_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX7523227.1"
                     /translation="MTPRKKSGKAKKPNEAPVSTAPEVQGGTATVGAGFPVVGIGASA
                     GGLAAIEAFFAAMPSDKEIGTAFVVVQHLSPDHKSILIDLVKRYTRMQVFQVEDGIEL
                     QPDCVYIIPPNRDMALLDGKLHLLDPGAPRGLRLPIDFFFRSLAQDRQEQAICIVLSG
                     TGTDGTLGLKAVKEEGGMAMAQAPESAAYDGMPRSAILTGLVDYVLPPEKMPERLFAY
                     VDHAFGGRQKPVVSHGPAATDLLQKVFVLLRAQTGHDFSLYKRNTIQRRVERRMAIAQ
                     IDRLEEYVRYLRETPLEADALFGELLIRVTSFFRDPEAFESLREHVIAPFFSKTSDGI
                     GRIWVPGCSTGEEAYSIAILIREQRDQRKKAFQVQIFATDIDIASIEKARVGVYPESI
                     AADVSSERLERFFVKKESSYRISKGIRDMVVFAKQDFIKDPPFSRLDLISCRNLLIYL
                     SGEAQKKILPMFHYALNQGGHLLLGNSETVGEFTKQFTLVDKKWKIYRQAGEASSRHV
                     TGPYLPPLEGAVPVMWPRSVRDPAHPAAVRDLAEQALLEGYIPASVLINPALEVLYIH
                     GRTGKYLEPAPGNAGLNLLDMAREGLRMELAAAARKVLALKTPVRCDGLQVKSNGDTS
                     VVNLIVQPVTKPEAARGLLMVIFEDVTPGPGPPVQEASEPVNDQEQRLATVERELRAK
                     EEYLQATIEELQTSNEELKSINEEAQSANEELQSANEELETSREELQSVNEELLTVNN
                     ELQTKIEELSQASNDMNNLLAGTNIGTIFVDHQKRIKRFTPAATQISNLIKTDIGRPV
                     GDIVSQLVGYDQLMEDTQAVLDTLIPKVAEVQSRSGQWYQMKIQPYRTVQNVIEGAVL
                     TFVEITREKELLEALRESERRNRETGGKR"
     gene            4560..6704
                     /locus_tag="VF357_03425"
     CDS             4560..6704
                     /locus_tag="VF357_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7523228.1"
                     /translation="MIKKGASRLDPSDLRRRAEERLRGKPAEPASISAEETRRLLHEF
                     RVHQIQLVMQNDELRKVQEELEGSRVKYFDLYDMAPVGYFTLSEKGSILEANLRAAQL
                     LGVEVNRLVKQPLTRYIVPEDQDIYYLHRKRLLEKGTREVCDLRMRRQDGSPFWARFE
                     TSTARDGEDGPLVWRLALSDMTDRHRVEEELKASKERLVEAQIVARLGYYGLDVATGT
                     WTGSNILDEVFGIGDDYRRDVEGWSLLVHPEDRQSMLDHFHNDVLGKKQPFDREYRIV
                     RPSDQCVRWVHGLGKVEYDSSGKPVKMNGTIQDITEYKKVEEEKRKLERHVQQAQKLE
                     SLGVLAGGIAHDFHNLLTVVLGHAELALNEISPMSPAHENLTAITTAARRAADLSLQM
                     LAYAGKAAFTVERVGLRELVEEMGHLLETAVSKKAILHLRLERGLPPIEADPSQIRQI
                     VMNLLINASEAIGDRDGVITVSVGATYCDAEYLQKTELHDDLAPGRYVHLEVTDTGHG
                     MDAETQSRIFEPFFSTKFTGRGLGLAAVQGIVRSHKGTIKVYSEPGKGTTFKVLFPAL
                     TEGGGDTRHPTESSPLADWRGKGTILLVEDEESLIALGARMLEHLGFTVLTAADGLQA
                     VELYRERGKEIDLVLMDLTMPHMDGAKAFSELRRLNPDVRVVLASGYTHEEVASRFAG
                     KDLSGVLQKPYTLARLRELLAGLLPEPPDGEE"
     gene            complement(6708..7013)
                     /locus_tag="VF357_03430"
     CDS             complement(6708..7013)
                     /locus_tag="VF357_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX7523229.1"
                     /translation="MAGGEIDLHGLTVAEALSRFATHYNARLRAGDTGPIRVIHGYGS
                     SGRGGDLRTALRELLSRHAGRLEFVAGETYFNNPGVTVIYPKHPLPAPSPILRTGRR"
     gene            complement(7021..7959)
                     /locus_tag="VF357_03435"
     CDS             complement(7021..7959)
                     /locus_tag="VF357_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX7523230.1"
                     /translation="MRKTISIPLLAALLFGVAGTPSFAFNQPPLNLGLTDILDGALPG
                     PGTYFTEYIQAYQSDRFKDHDGNTIPGDPKIANVLSMNQFVHVYKHKILGGHLGADLL
                     LPVVAISGSGTVGSPGPPVSTNPAVLGDLVVGPFLQWFDTKLLGRPLFQRVELDFTLP
                     TGQYDRKYLINPGSNLWTIEPYYAFTWFLTPKFSTSWRIHYTYNTKNNDPFAALGAND
                     LQPGQAFHFNYSFEYEFFKNFRGAVAGYYLKQISNDKVDGNPQTDTKEQVFAVGPAVS
                     WAASPNFFLGLKTQWETSAENRPEGNRTTFRMTYKF"
BASE COUNT         1574 a         2390 c         2694 g         1430 t
ORIGIN      
        1 gccgtgaacc gaccgagggt acggaacgcg acgagctgcg cgtgctggag ccgcgtgcgg
       61 acgaaggcgt cgggtgggat cttcacccgg accccggccc cccacgtcga gggtggaggg
      121 cgtggtccga aggatgtcgt agaccagctc cgtggaaccg gtccggaccc tgctccccac
      181 gtccagaccg gggtttccgg gatcttccgg ggtgatggcg ttcggatcgc cccgggtttc
      241 ccccgagaag accagcttcc accgtttctt gagcctcgga aggcgcaggg tggcgcgaat
      301 cgtactgcgg aaggagaagc gctcctcttc gtcccaccgg aaatcgttta tccaccgcag
      361 gaacgactcc ggaggatcga tgacctccat ccgtttgtcg ccgaagaatt ggtcgaacca
      421 tacgacggtt tcgaacaggt ccctctcgac ccggtcgcga gcgatgcgcc ggttgccaag
      481 gaaaggagga acgccgccgc caatgccggg aaaaccaacc tgcggcggcg catgctcttc
      541 ggcggcggta tcatcttcct ccgcggggca cggtgccgat ggaggcggcg gtcgttactg
      601 ctttacggtc gcctcctcga cgatcacctt gtacttgtac ccggccccga aatccttgtc
      661 cttggaaagg atacctttcg ccgtcaccac gtccccgacc ttcggaacgt cctgcgtggt
      721 gaccaccagg ttattcgttc ccttccccgg atccccgctg ccgtcgcgga ggtggaccca
      781 gttctttccc atgatcccct tggagacttt gacgaccttt ccccggacga cgacggtctt
      841 cttgtccagt ttctccgctt tttcataggt ttcggccacg ttaaccgcgt ccgctccctt
      901 cgccttttcc accttgatct gttcggaggt ggctggcgcc ttcgcgcctt cgttcccgcc
      961 gctcgccgca ttcccgaacg ccaacgcccc gatggaaacg caaagagcga cccctttacc
     1021 gaaacgattc atccgcatcc cctttccggg tttttccgga tatcttacca ccctcgtccc
     1081 ggccttgtct gccggaggtg ccggtcgggg cgggacgaga agattatccc gttctccgct
     1141 aaactatgcg cgggggcggt cttgtcccga gggccgccga agattcgaag gggggagtcc
     1201 cgatgcgcgt ggtggcgttc aatggcagcg cccggaaggg cgggaacacg accttgctcc
     1261 ttcggcacgt gttcgcggaa ctcgagaagg gggggatcga tacggagctc gtggaactgg
     1321 ccggaaagag gatccacggg tgcaccgcct gctacaaatg cttcgcgaac aaggaccgcc
     1381 ggtgcgcggt gaagggggac gcggtgaacg actgcataga gaagatcgac ggcgccgacg
     1441 gcatcctgct gggatccccc acctacttca ccgacgtcac cgcggagatg aaggcgctga
     1501 tcgaccgggc ggggatggtc gggcgggcca atggaaacat gtaccggcgg aaagccgggg
     1561 cggccgtggt ggcggtgcgc cgcaccggcg cgatccacgc cttcgacacg atgaaccact
     1621 tctttctcat cggggagatg atcatccccg ggtcgagcta ctggaacatc ggggtcggtc
     1681 gagagatcgg cgacgtggag aaggacgagg aaggggtgca gacgatgcgc accctcgggg
     1741 agaacatggc gtggctgatg aaggcgcttg cggcggccca acggggataa tatccgcagg
     1801 gatcgggcga tctcgtcgtc atcgatcatg tttccctttt gccgctgttc gccgtaatat
     1861 aacaatattc ggattgcgga ccccgcgctt taaggcgagg cgatcatgac ccccagaaaa
     1921 aaaagcggga aggcgaagaa gccgaacgag gctcccgttt ccacggcacc cgaggtgcag
     1981 ggcggaactg cgaccgtcgg agccgggttt cccgtcgtcg gcatcggcgc gtccgcaggg
     2041 gggctggcgg ccatcgaagc gttctttgcc gccatgccga gcgacaagga gatcgggacg
     2101 gcctttgtcg tggtgcagca cctctccccc gaccacaaga gcatcctgat cgacctggtc
     2161 aagcggtaca ccagaatgca ggtcttccag gtggaggatg ggatcgagct gcagcccgac
     2221 tgcgtctaca tcattcctcc caatcgggat atggcgttac tggacggcaa actgcatctg
     2281 ctggacccag gtgcgccgcg tggactccgc cttccgatcg atttcttctt tcgctcgctg
     2341 gcgcaggacc ggcaggagca ggcgatctgc atcgtcctct cgggcaccgg caccgacggc
     2401 accctcggac tcaaggcggt caaggaagaa ggcggcatgg cgatggccca ggctccggag
     2461 tcggctgcgt acgacgggat gccgcgcagc gccatcctca ccggattggt ggactatgtc
     2521 cttccgccgg agaaaatgcc ggagcggttg ttcgcgtacg tggatcacgc cttcggaggc
     2581 cggcagaaac cggtcgtatc tcacggtccc gccgccaccg acctccttca gaaggtcttc
     2641 gttctgctgc gcgcccagac gggtcacgat ttttcgctgt acaaacggaa caccatccag
     2701 cgccgcgtcg aacggcgcat ggccatcgcc cagatcgacc ggctcgagga gtatgtccgc
     2761 tatctgcggg aaactccgtt ggaggcggac gcgctttttg gggaactgct gatccgcgtc
     2821 accagctttt ttcgcgaccc ggaagcgttc gagtcgcttc gagagcacgt catcgccccc
     2881 ttcttctcca agacctccga tgggatcggg cggatctggg tgcccggctg ttccaccggc
     2941 gaagaggcgt actcgatcgc catcctgatc cgggaacaac gggaccaaag gaaaaaggcc
     3001 ttccaggttc agatcttcgc gaccgatatc gacatcgcat cgatcgaaaa agcgcgcgtc
     3061 ggggtctacc cggaaagcat cgccgcggat gtatcgtccg aacgactgga gcgcttcttc
     3121 gtcaagaagg aaagttccta ccggatcagc aaaggtatcc gcgacatggt ggtgttcgcc
     3181 aagcaggatt tcatcaaaga ccctcctttt tccaggctcg atctgatcag ttgccggaac
     3241 cttctgatct atttgagcgg cgaggcgcag aaaaagatcc tgccgatgtt ccattacgcg
     3301 ttgaaccagg gcggacacct cctgttgggg aactcggaaa cggtcggcga attcacgaaa
     3361 cagttcaccc tggtcgacaa aaagtggaag atctatcgac aggcagggga ggcgtcgtcc
     3421 cgccacgtta ccggccccta cctgccgccc ctggagggag cggttccggt catgtggccc
     3481 cgttccgtgc gcgatccggc gcacccggcc gcggtccgcg acctggcgga acaggcgttg
     3541 ctcgaagggt atatccccgc gagcgttctc atcaatcctg cgctcgaggt gctgtacatc
     3601 catggccgca ccggcaaata cctggaaccg gcgccgggaa atgccggttt gaacctgctg
     3661 gacatggcgc gcgagggatt acggatggaa ttggccgccg cggcgcgcaa agttctcgcc
     3721 ctgaaaacgc ccgtccgctg cgacgggttg caggtcaagt cgaacgggga cacctccgtc
     3781 gtgaatctca tcgtccaacc ggtgacgaaa ccggaggcgg cgcgcggctt gctgatggtc
     3841 atcttcgagg acgtgacccc tggacctggt ccgccggtgc aagaggcgtc cgaacccgtc
     3901 aacgatcagg aacagcgcct cgccacggtg gaacgggaac tgcgtgcaaa ggaagagtac
     3961 ctgcaggcca ccatcgagga actgcagacc tccaacgaag aattaaagtc catcaatgaa
     4021 gaggcgcaat cggcgaacga agagttgcaa tccgccaacg aggaactgga gacttccagg
     4081 gaagagttgc agtcggtcaa cgaggagttg ttgaccgtca acaacgaact gcagaccaag
     4141 atcgaggagt tgtcccaggc gagcaacgac atgaacaacc tgctggcggg cacgaacatc
     4201 gggacgatct tcgtggatca ccagaagcgc atcaagcggt ttacgccggc ggcgacgcaa
     4261 atcagcaacc tcataaagac cgacatcggg cgtcccgtgg gcgacatcgt ctcgcagttg
     4321 gtcggctacg accaactgat ggaggacacg caggcggtgc tggatacgct gatccccaag
     4381 gtggcggaag tgcagagcag gagcgggcag tggtaccaga tgaaaatcca gccctatcgt
     4441 acggtccaga acgtcatcga gggagcggtg ctgaccttcg tcgaaatcac gagggagaag
     4501 gaattgctgg aggcgttgcg cgagagtgag cgccggaaca gggaaacggg aggcaagcga
     4561 tgattaagaa gggcgcgagc cggttggacc cttccgacct gcggcgtcgg gcggaggaac
     4621 ggttgcgggg aaaacccgcc gagccggcct ccatctcggc ggaagaaacc cggcggctac
     4681 tccacgaatt tcgggtgcac cagatccagc tggtgatgca gaacgacgag ttgcgcaagg
     4741 tgcaagagga actcgaaggg tcgcgcgtca agtacttcga cctgtacgac atggcgccgg
     4801 tggggtattt caccctcagc gagaagggat cgatcctcga ggcgaatctc agggccgccc
     4861 agttgctggg cgtggaagtg aatcggttgg tcaagcagcc gctgacccgc tatatcgttc
     4921 cggaggatca ggacatctac tacctgcacc gcaaacgcct tcttgaaaag ggcacgcggg
     4981 aggtgtgcga tctgaggatg cggcgtcagg acggctcccc gttctgggcg cggttcgaga
     5041 cgagcaccgc tcgggatggt gaagatgggc cgcttgtgtg gcgtctcgcg ttgagcgaca
     5101 tgaccgatcg tcatcgtgtg gaagaggagt taaaggccag caaggagcgg ttggtcgagg
     5161 cgcagatcgt cgcacggctt ggatactacg gcctcgatgt ggccacaggg acgtggaccg
     5221 gttcgaacat tctcgacgag gttttcggca tcggggacga ttaccggcgg gacgtcgaag
     5281 ggtggtctct tctcgtccac ccggaggatc ggcaatccat gctggatcac ttccacaacg
     5341 atgtcttggg gaagaagcaa ccgttcgacc gcgagtatcg gatcgtcaga ccgtcggatc
     5401 aatgcgtccg ttgggtccac gggctgggca aggtcgagta cgattccagc ggcaagcccg
     5461 taaaaatgaa cggaacgatc caggacatca cggagtacaa gaaggtggag gaggagaagc
     5521 ggaagctgga gcggcatgtg cagcaggcgc agaaactgga aagcctgggc gtcctggccg
     5581 gcgggatcgc gcacgatttc cacaatctcc tcacggtcgt tctggggcac gccgagctgg
     5641 ccctcaatga aatctctccg atgtcccccg cccacgagaa cctcaccgcg atcacgaccg
     5701 cggcgcggcg tgccgccgat ctgtccctcc agatgctggc atacgccggc aaggcggcct
     5761 tcacggtgga gcgggtgggg ctgcgggagc tggtcgagga gatggggcac ctgctcgaaa
     5821 ccgcggtctc gaagaaggcg atcctgcacc tgcgccttga gcgcggtctt ccgccgatcg
     5881 aggcggaccc gagccagatc cggcagatcg tgatgaacct gctcatcaac gcgtcggaag
     5941 cgatcggcga ccgggacggg gtgattaccg tgtcggtggg cgcgacctat tgcgacgcgg
     6001 agtacctgca aaagacggaa ctgcacgacg acctcgcccc ggggcggtac gtgcacctgg
     6061 aggtgaccga caccggccac gggatggacg ccgagacgca atcgcgcatc ttcgagccgt
     6121 tcttctccac gaagttcacg ggtcgggggc tgggactggc cgcggtgcag gggatcgtcc
     6181 gctcgcacaa ggggacgatc aaggtgtaca gcgaaccggg gaaggggacg acgttcaagg
     6241 tcctctttcc ggcactgacg gaaggggggg gggatacccg ccatcccacg gagtcttccc
     6301 cactggccga ttggcgtgga aagggaacga tcctgctggt ggaagacgag gagagcctga
     6361 tcgccctcgg ggcgcggatg ctggagcacc tgggtttcac cgtgctgacc gcggcggacg
     6421 ggctgcaggc ggtggagctt tatcgggaac gggggaaaga gatcgacctg gtgctgatgg
     6481 acctgacgat gccgcacatg gacggcgcga aggcgttcag tgagctgcgc cggctgaacc
     6541 cggacgtccg tgtcgttctt gccagcgggt acacccacga agaggtggcg tcccgcttcg
     6601 cggggaagga cctttcaggg gtattgcaga aaccgtacac cctcgccagg ctgcgggaat
     6661 tgctcgccgg gctgctgccg gaacctccgg acggggagga gtgacgtcta ccggcgtccc
     6721 gttcggagga tcggggaagg cgcggggagc gggtgcttcg ggtagatgac cgtcacgccg
     6781 gggttgttga agtacgtctc gccggcgacg aattcgaggc ggccggcgtg gcgggagagc
     6841 agctcacgga gcgcggtccg caggtcgccg ccgcggcccg aagagccgta gccgtggatc
     6901 acgcggatcg gaccggtgtc ccccgcccgc agccgcgcgt tgtaatgggt ggcgaagcgg
     6961 gagagcgctt ccgccacggt cagcccgtgc aggtcgatct ccccgcccgc catcgggcga
     7021 tcagaacttg tacgtcatcc ggaacgtggt ccggtttccc tcgggacggt tttccgcgga
     7081 ggtctcccac tgggtcttca ggcccaggaa gaagttcggg gacgcggccc aggagaccgc
     7141 cggtccgacg gcgaagacct gctccttcgt gtccgtctgc ggattgccgt cgaccttgtc
     7201 gttggagatc tgcttcaggt agtaccccgc caccgctccc cggaaattct tgaagaactc
     7261 gtattcgaac gagtagttga aatggaacgc ctgccccggc tggaggtcgt tcgctcccag
     7321 agcggcgaag ggatcgttgt tcttcgtgtt gtaggtgtag tggatccgcc aggaggtgga
     7381 gaacttcgga gtgaggaacc aggtgaaggc gtaatacggt tcgatcgtcc agaggttgga
     7441 cccggggttg atcaggtact tcctgtcgta ctggccggtc ggcagggtga agtccagttc
     7501 caccctctgg aacaaggggc ggccgaggag cttcgtgtcg aaccactgga ggaaggggcc
     7561 gacgaccaga tcgccgagga ccgcggggtt ggtcgacacc ggtgggccgg gggagccgac
     7621 ggttcccgag ccgctgattg ccaccaccgg caggaggagg tccgcaccga ggtgtccccc
     7681 caggatcttg tgcttgtaga catggacgaa ctggttcatt gaaagcacgt tggcgatctt
     7741 cgggtccccg gggatcgtgt ttccgtcatg gtccttgaat ctgtccgact ggtacgcctg
     7801 gatgtactcg gtgaagtagg tcccggggcc gggaagggct ccgtcgagga tgtccgtcag
     7861 gccgaggttc agcggcggct gattgaacgc gaacgatggg gtgccggcga caccgaagag
     7921 cagggccgcc aggagcggaa tggagatcgt cttgcgcatc gggtccctcc gaacggatgg
     7981 atcggttccg gatccctgaa tcggactcaa catacatacc cgcaacccgg cagggccgtc
     8041 aaccgattcc tgcggagcga ctggtcagga aagcccgttc gcatcccg
//
LOCUS       DASVJL010000169         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_588552, whole genome shotgun sequence.
ACCESSION   DASVJL010000169 DASVJL010000000
VERSION     DASVJL010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588552"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VF357_03440"
     CDS             <1..214
                     /locus_tag="VF357_03440"
                     /inference="COORDINATES: protein motif:HMM:NF017317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEX7523231.1"
                     /translation="FGGGPVGVETISASVSEERDTIEDVYEPFLIQRGFLKRTPRGRV
                     ATPAAWKHLGIAVPKTSAQDSLFGET"
     gene            211..774
                     /locus_tag="VF357_03445"
     CDS             211..774
                     /locus_tag="VF357_03445"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEX7523232.1"
                     /translation="MTPVRVLIALFVLSLLGFPPGSSLAAPPAPGISDVSGTIHGGEA
                     RVSFTLRNAFTPEMVEALKSGIEITFKTTVEVERVYRNWFNRRMGHVQYTRSVRYDAL
                     AGVYRLHRDDGDELLPDVLAALERMTRFEVAVPVAGDVEKGKSYRASVHARLDKVGLS
                     EPLRSIFFFSSLWDIETGWSRGALQTP"
     gene            771..>1722
                     /locus_tag="VF357_03450"
     CDS             771..>1722
                     /locus_tag="VF357_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523233.1"
                     /translation="MNAPPAGQISPSPGAEPAKRRRERWAIGIVAFLVAVLTFLEAHL
                     AAVGGAVTFSSNIIIFALINLNVIFVVLLIFLVTRNVFKILLDRRRHLLGAKLRSRLV
                     LIFICFSLIPTMLLFIAATNITTTSIKSWIGGRVGQALTGAIEIARERLEEDARALTP
                     AAERAAAAVSDTVDPASFPRVLKSARSLAPAGTGLLFFEKGAEVARDGDLPPRVGAEI
                     LNRVKGMKEAADGKGSGTLIGDKFAAAWRRTPGGVIVVAVRPLLPAEAARILEITRAY
                     DEYHQVRLLDDPIRASYVGILILITLLIVFAASWMGIYLAR"
BASE COUNT          262 a          602 c          549 g          309 t
ORIGIN      
        1 gttcggggga ggccccgtcg gcgtggagac gatctccgcc tccgtcagcg aggagcggga
       61 cacgatcgag gatgtctacg agccgtttct catccagcgg ggtttcctca agcgcactcc
      121 gcggggaagg gtggccaccc cagccgcgtg gaagcatctc gggatcgcgg tcccgaagac
      181 gtccgcccag gattcgttgt tcggagagac gtgacccccg tgcgcgtcct gattgccctt
      241 ttcgtcctgt ctctcctcgg gttccctccc gggagctccc ttgccgcgcc gcccgccccc
      301 gggatctcgg atgtttccgg gacgattcat ggcggcgagg cccgggtcag cttcaccctc
      361 cggaacgcgt ttacccccga aatggtcgaa gcgttgaagt ccgggatcga gatcaccttc
      421 aagacgaccg tcgaggtcga gcgcgtctat agaaactggt tcaatcggcg gatggggcat
      481 gtccagtaca cccgctccgt ccggtacgac gccctggcag gcgtctaccg gcttcaccgc
      541 gacgacggtg acgagctgct gccggacgtc cttgcggctc ttgaacggat gacccggttc
      601 gaggtggccg tcccggtggc cggagacgtg gaaaagggaa aatcgtaccg ggcaagcgtc
      661 cacgcccggc tggacaaggt ggggctttcc gagccgcttc gatccatctt cttcttctcc
      721 tccctgtggg acatcgagac cggctggtcc cgcggggccc tccagactcc gtgaacgccc
      781 ctccggccgg acagatttcc ccttcgccgg gagcggagcc cgcgaagcgg cgcagggagc
      841 ggtgggccat cgggatcgtc gcgttccttg tcgcggtcct gacgttcctc gaggcccacc
      901 tggcggcggt cggcggagcg gtcacgttct ccagcaacat catcatcttc gcgctgatca
      961 acctgaacgt catcttcgtc gtcctcctga tcttcctcgt cacgcggaac gtcttcaaga
     1021 tcctcctgga ccggcgacgg cacctcctgg gagcgaagct ccggtcccgg ctcgtcctca
     1081 tcttcatctg cttctccctc atcccgacga tgctcctctt catcgccgcg acgaacatca
     1141 cgacgaccag catcaagtcg tggatcggcg gcagggtggg acaggccctc accggagcca
     1201 tcgagatcgc cagggaacgc ctggaggagg acgcgcgggc cctcaccccg gccgcggagc
     1261 gcgccgctgc ggcggtgtcg gacacggtcg accccgcttc cttcccgcgg gttctcaaat
     1321 cggcccggag cctggccccc gccggtaccg gcctgctctt cttcgagaag ggcgccgaag
     1381 tcgcccgcga cggcgatctc ccgccgcggg tcggggcgga gatcctcaat cgggtcaagg
     1441 ggatgaagga ggccgcggac ggaaaggggt cggggaccct gatcggggac aagttcgccg
     1501 ccgcctggcg tcgcaccccc ggcggcgtca tcgtcgtggc ggttcgacct ctcctccccg
     1561 ccgaggccgc gcgaatcctc gaaatcacaa gggcgtacga cgagtaccac caggtgcggc
     1621 tcctggatga cccgatccgg gccagctacg tcggcatcct catcctcatc acgctcctga
     1681 tcgtcttcgc cgcctcctgg atggggatct accttgcacg gc
//
LOCUS       DASVJL010000170         8233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_213671, whole genome shotgun sequence.
ACCESSION   DASVJL010000170 DASVJL010000000
VERSION     DASVJL010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213671"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VF357_03455"
     CDS             complement(<1..334)
                     /locus_tag="VF357_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX7523234.1"
                     /translation="MDNDALLDAFLLHLKTERRLSGNTLSAYGADLHRFSVFLADRGI
                     DARKFLRPDFLDHLTALRDGGLSARSTARHVSTLRSFFRFLVREGVLAASPVSEVKAP
                     RIGRPLPKY"
     gene            complement(334..834)
                     /locus_tag="VF357_03460"
     CDS             complement(334..834)
                     /locus_tag="VF357_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7523235.1"
                     /translation="MKVGILSDSHDHRGAAEGALLLFRAEGVGMVFHLGDVCSPVVLA
                     GYADPAIPLRGVFGNNDSDRDGLQEATGGAFRQGPRIETVNGRKILLAHSYDQLRGEL
                     SGQGRFDLVLFGHTHRPLTMRVGKALVVNPGESCGLMRGKSTCAIVDLATMEPRIVEI
                     PLPGTG"
     gene            complement(845..3520)
                     /gene="glnD"
                     /locus_tag="VF357_03465"
     CDS             complement(845..3520)
                     /gene="glnD"
                     /locus_tag="VF357_03465"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX7523236.1"
                     /translation="MPLPSLSQDLLRTDLPDRDFLDSLKGFLAGTHIAVREEQRQSLC
                     GGAEACRKISGAMDAVLSVLHDRARARFRASAPDMSYRLAVVAVGGYGRRELCPHSDV
                     DLLFLHGYKIDRFVEAMTEWMLYPLWDLGLEVGHSVRNTKETIRLAGADDSIRTALLD
                     HRLVAGDDSLYGESTRELDRFLYFQGGDKFIGKKIGEMRSRHAKVGSTVYLLEPNVKE
                     GRGGLRDLQTAVWGARIKYKCDDLEELRKKGVVGARTVEAIRHVLDYLLRVRNELHYL
                     QGKKADVLGFEVQERLAERFGYRPVGANQAVERFMRAYYLHAASAARLSDEVIEEVGR
                     FLPEEGSRKPFFFLKRKLIGDGGILYKGQLQVKDRASFEKEPIRILEFFRSMQKTRSV
                     LSPQARRNIQLALPAVGTAFREDREAASLFLEILADPVHLRETLLAMNESRFLARYIP
                     EFAPLYCKVQRDIYHIYTVDIHSIRCASVLAEIETSASRTGDEEEFHRIHATVRNPGL
                     FHLAILFHDIGKGKGQGHSRIGAEIVARIGARWGLEEQDVSDLVFLVEQHLLMANVSQ
                     RRDLHDIELILSFADTVGTASRLDQLYLLTYADMKEVAPEVWTQWKAMLLAELYEKGR
                     NVLDHGALKRPFEERAHRRRQQVREFLGGEPRETTDRFLARVDDRYLLATPDGRFVEH
                     CRTLAGFDGKTPVIMVIDLPESGTTEFLVVCPDERGLFAKIAGTLSANSMNILNASIA
                     TTVDAVALDTFYANYLGKSLQGDPKKERVIAELSRVLRGETTVEALMADPRAGKFVRD
                     RVPRYRPTRVVFDNTVSSRCTVVDIFTYDRIGLLYDITSTLTSLGIDIALSKISTKAD
                     QVADVFYIVDRDGGKISASDRQEEIRQALLASIGA"
     gene            complement(3521..4876)
                     /locus_tag="VF357_03470"
     CDS             complement(3521..4876)
                     /locus_tag="VF357_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7523237.1"
                     /translation="MTKLRRHLCAAFLAVFLCAAGLPFDAPPAADAAPGVSDIRAWTN
                     EIYTRVAIDTGEEVSWQANTVRADPSRGLPPRIFIDIRAAGIRDEILRQPVVVHNGLL
                     RQVRAGRFDRDTVRVVIDLERESTYRVFALSGPFRIIVDIDGEGEIPALPAPPDAVVP
                     PGPVAAGPSPEDPTPAPPPVTPPVFPPSPSTGPVVPSSPPAIPPSVESPGAAPSYPSP
                     TRKHRVRVMIDPGHGGKDPGATGPTGLKEKDVVLAIGRKIREKLSRSGEFDVRMTRDD
                     DVFIPLEERTAMANKGGVDLFVSLHINASRNRRTEGFSTYVLSRGASNREDLELAARE
                     NGVPVAKLQGVKFIIDDMFTGARKNESLRLAKTVNDAVVRHVSTRYPGAQSLGLKQAP
                     FYVLVGARMTAVLVEASFISNLREESRLRDPSCLDGIADGVVEAVRYYGQNGILAHSD
                     Y"
     gene            complement(4873..7461)
                     /gene="mutS"
                     /locus_tag="VF357_03475"
     CDS             complement(4873..7461)
                     /gene="mutS"
                     /locus_tag="VF357_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX7523238.1"
                     /translation="MSLPLTPAMRQYVEIKSRYRDCILFFRMGDFYEMFFEDALRASR
                     LLDIALTSRDKEANIPMCGVPHHARNAYLSKLIRQGCKVAICEQIEEPGQKGIFRREV
                     TEVVTPGLVFNEECLDARGNNFLAAVRFVAPFACAALDATTGEFFHETCDTEEALADA
                     LFRIAPAEFVALEGEGNPTTTRGKRLLEGKLLTLLSPSAVAVFPPPPEIGGIPPTDHP
                     SGGVVRAVLYYLFLHQPTALSEIVRVTEREGRRYLAMDETAVRTLEIFSTMSGERKGS
                     LLWAVDRTRTPMGARMLRAWLAAPLLDVERIGERHDAVGELLEAHAIRKSLSPPFDAM
                     GDLSRLASRLAQDRSGPRDVAALRDHLAAIPAIKIALGEPSAMRLRSVKGLLGDHAPV
                     VDRISSALVDPPPAGYKEGGVFRPGYDTQVDELTHLLTHGKEMLAEMEARERQRTGIG
                     GLKVGYNRVFGYYIEVTRAHLEKVPADYIRKQTLANAERYITPELKEFEGRVLRAQEG
                     RAAREEELFLALRDSLKGALPAVYAAADGIAELDALLSFAELAAENGYGRPRVNGGRE
                     ILIENGRHPVVEKILGRHAFVPNDCVLSPEGTRLAVLTGPNMAGKSTYIRQAALIVLL
                     AHVGSFVPADRAEIGLVDRIFTRIGASDDLSRGDSTFMVEMRETARILGGVTDRTLVV
                     LDEVGRGTSTYDGLSIAWAVAEHLHDSPARPKVLFATHFHELTDIVSTCANARNFHVA
                     VREWQGEIIFLRRIDEGSASKSYGIQVARLAGLPASVVDRARDILKNLESAEYNEYGL
                     PTLAGARVVTESANAQMELFSRRARGDENAVLDQIRRCEPERLSPLDALMRLAEWKER
                     LGKGST"
     gene            complement(7496..7969)
                     /locus_tag="VF357_03480"
     CDS             complement(7496..7969)
                     /locus_tag="VF357_03480"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX7523239.1"
                     /translation="MEYLRQAGGGGGTAPCVFCVRDGDLEDPERLVVGLFPDTVAILN
                     RYPYNNGHVLIAPRRHVANLWDLPSEELHELFSLVSIGTRALAVEYRTEGMNIGMNLG
                     KAAGAGIVDHLHVHLVPRWAGDTNFMTPVQETRVLPESLLETRRRLSAVFGLLRP"
     gene            complement(7999..>8233)
                     /locus_tag="VF357_03485"
     CDS             complement(7999..>8233)
                     /locus_tag="VF357_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7523240.1"
                     /translation="NLTKKECEVIVDTVFLNMKDALHRGEKIEIRGFGSFTVRTRRAK
                     EGRNPKTGEKVAIPEKRIPFFKVGKELREMVNG"
BASE COUNT         1306 a         2800 c         2814 g         1313 t
ORIGIN      
        1 ggtatttcgg caggggtcgc ccgatccgcg gcgccttgac ctccgagacg ggagacgcgg
       61 cgaggacccc ctcgcgcacg aggaaccgga agaaggagcg cagcgtcgag acgtgccggg
      121 cggtggaacg ggccgaaagc cccccgtccc ggagcgccgt gaggtggtcg aggaagtcgg
      181 ggcggaggaa cttcctcgcg tcgatcccgc ggtccgcgag gaagacggag aagcggtgca
      241 ggtcggcgcc gtacgccgat agcgtgttcc ccgagagacg ccgctccgtc ttcagatgga
      301 gaagaaaggc gtcgagaagg gcgtcgttgt ccatcagccg gtccctggga gggggatctc
      361 cacgatccgc ggctccatcg tggccaggtc gacgatcgcg cacgtgctct tcccccgcat
      421 gaggccgcac gactcccccg ggttcaccac cagcgccttt cccacccgca tcgtgagcgg
      481 ccggtgcgtg tggccgaaga ggacgagatc gaaccgtccc tgcccggaga gctccccccg
      541 cagctggtcg taggaatgcg cgaggaggat cttccgcccg ttgaccgttt cgatccgcgg
      601 cccctgccgg aacgcccccc cggtcgcctc ctgcagcccg tcgcgatcgg agtcgttgtt
      661 gccgaagacc ccgcgcagcg ggatcgccgg atcggcgtac cccgcaagga cgaccggaga
      721 gcagacatcc ccgagatgga acaccatccc caccccctcg gcgcgaaaaa ggaggagcgc
      781 gccttccgcg gcgccccggt ggtcgtggga gtctgagagg atccccacct tcacggcgaa
      841 atcactaggc gccgatggac gcgaggagcg cctgacggat ctcctcctgg cgatcggaag
      901 ccgagatctt ccccccgtcc cggtcgacga tgtagaagac gtcggccacc tggtccgctt
      961 tcgtggagat tttggagagc gcgatgtcga tccccagcga cgtcagcgtg ctcgtgatgt
     1021 cgtagaggag cccgatccgg tcgtaggtga agatgtcgac cacggtgcag cgcgaggaga
     1081 ccgtgttgtc gaaaacgacc cgggtgggcc ggtaccgggg gacacggtcc cggacgaact
     1141 tccccgcccg cgggtcggcc atgagcgcct ccacggtggt ctccccgcga aggacgcgcg
     1201 aaagttccgc gatgacgcgc tccttcttcg ggtccccctg cagggacttt ccgagatagt
     1261 tggcgtagaa cgtgtccagc gccacagcgt ccacggtggt ggcgatcgag gcgttgagga
     1321 tgttcatgct gttggccgac agcgtccccg cgatcttcgc gaagaggccg cgctcgtcgg
     1381 ggcagacgac gaggaactcc gtggtccccg attccggcag gtcgatcacc atgatgacgg
     1441 gcgtcttccc gtcgaacccc gcgagggtcc ggcaatgctc cacgaaccgg ccatcgggag
     1501 ttgccaggag ataccgatcg tccacgcggg cgaggaagcg atccgtcgtc tcccgaggct
     1561 ccccgcccag aaactcgcgc acctgctggc gccgccggtg cgcccgctcc tcgaacgggc
     1621 gctttagtgc cccatggtcc aggacgttgc gccccttctc gtacagctcg gccaggagca
     1681 tcgccttcca ctgggtccag acctccggcg ccacctcttt catgtccgcg taggtgagca
     1741 ggtagagctg gtccagccgg gaggccgttc ccacggtgtc ggcgaaggag aggatcaatt
     1801 cgatgtcgtg caggtcccgc cgctgcgaaa cgttggccat cagcaggtgc tgctccacga
     1861 ggaagaccag gtccgatacg tcctgttcct cgagtcccca ccgggcgccg atccgcgcca
     1921 cgatctccgc gccgatcctg ctgtggccct gccccttccc cttcccgatg tcgtggaaca
     1981 ggatcgcgag gtggaacagc ccggggttcc gcacggtcgc gtggatccgg tggaactcct
     2041 cctcatcccc cgtgcgcgaa gcggacgtct cgatctccgc caggacgctc gcacagcgga
     2101 tggagtggat gtccaccgtg tagatgtgat agatgtcccg ctgcaccttg cagtagagcg
     2161 gcgcgaactc ggggatgtac cgggcgagga accggctctc gttcatggcg aggagggtct
     2221 ctctcaagtg aaccgggtcg gcgagaatct cgaggaagag gctcgccgcc tcccggtcct
     2281 cgcggaacgc ggtccccacg gcgggcaacg ccagctggat gttccgccgg gcctgcgggg
     2341 agaggaccga tctcgtcttc tgcatcgacc ggaagaactc gaggatccgg atcggctcct
     2401 tctcgaagga ggcgcggtcc ttgacctgca gctgcccctt gtagaggatc cccccatcgc
     2461 cgatgagctt ccgcttcagg aagaagaacg gctttcggct cccctcctcc ggcaggaacc
     2521 gccccacctc ctcgatgacc tcgtcggaga gccgggccgc ggaggccgcg tggaggtagt
     2581 acgcccgcat gaaccgctcg acggcctggt tcgccccgac cggccggtag ccgaaccgct
     2641 ccgccaaccg ctcctgcacc tcgaagccga ggacgtcggc tttcttcccc tgcaggtagt
     2701 ggagctcgtt tcgcacccgg agaaggtagt cgaggacgtg ccggatcgcc tcgacggtcc
     2761 gggctcccac cacgcccttc ttccgcaact cctccaggtc gtcgcacttg tacttgatcc
     2821 gcgcccccca taccgcggtc tgcagatcgc gcagcccccc gcgcccctcc ttgacgttcg
     2881 gctccagcag gtacaccgtc gagccgacct tggcgtgccg cgagcgcatc tccccgatct
     2941 tcttcccgat gaacttgtcg ccgccctgga agtagaggaa ccggtccagc tcccgggtcg
     3001 actcgccgta cagggagtcg tcccccgcga cgagccggtg gtcgaggagc gccgtgcgga
     3061 tcgagtcgtc cgcgccggcg agccggatcg tctccttggt gtttcggacg ctgtgcccca
     3121 cctcgaggcc caggtcccac agcgggtaga gcatccactc ggtcattgcc tcgacgaagc
     3181 ggtcgatctt gtagccgtga aggaaaagaa ggtccacgtc ggagtggggg cacagctcgc
     3241 gccgcccgta gccgcccacc gctacgaccg ccaaccggta cgacatgtcc ggtgccgagg
     3301 cgcggaatcg cgcgcgggcg cggtcgtgga gaaccgacag gacggcgtcc atggcgccgg
     3361 agatctttcg gcacgcctcg gccccgccgc agaggctttg ccgctgctcc tcgcgaaccg
     3421 cgatgtgggt gccggcgagg aaccccttca agctgtcgag gaagtccctg tcgggaaggt
     3481 cggtccgcag aaggtcctgg gaaagggatg gaagaggcac tcagtaatcc gagtgggcga
     3541 ggatcccgtt ctgcccgtag tagcggaccg cctccaccac gccgtcggcg atcccgtcga
     3601 ggcacgaggg atcgcgcagg cgggactcct cgcggaggtt gctgatgaag gaggcctcga
     3661 ccaggacggc cgtcatccgg gcgcccacga gcacgtagaa cggggcctgc ttcagcccga
     3721 ggctctgcgc ccccgggtaa cgggtggaga cgtggcggac gacggcgtcg ttcaccgtct
     3781 ttgccaggcg cagcgactcg ttcttgcgcg ccccggtgaa catgtcgtcg atgatgaact
     3841 tgaccccctg gagcttggcg accggcacgc cgttctcccg ggcggccagt tcgaggtctt
     3901 cccggttcga cgcgccgcgg gagaggacgt aggtggagaa cccctccgtc ctccggttcc
     3961 ggctggcgtt gatgtggagg gagacgaaga ggtcgacgcc tcccttgttc gccatcgccg
     4021 tgcgctcctc cagcgggatg aagacgtcgt cgtcgcgggt catccgcacg tcgaactccc
     4081 cggagcgcga cagcttctcg cggatcttcc ggccgatcgc caacaccacg tccttctctt
     4141 tcagcccggt cggaccggtc gccccagggt ccttcccgcc gtggcccggg tcgatcatca
     4201 cccgcacgcg gtgcttccgg gtcggggagg ggtaggaagg ggcggcgccc ggcgattcca
     4261 ccgacggcgg gatcgccggg ggcgaggaag gaaccaccgg accggtggac ggcgaagggg
     4321 ggaaaacggg cggtgtcacc gggggcggag ccggagtcgg atcctccggt gacggacccg
     4381 cggcgacggg accgggggga acgacggcgt ccgggggcgc cgggagagcg gggatctccc
     4441 cttccccgtc gatatccacg atgatccgga acgggccgga gagcgcgaag acgcggtagg
     4501 tgctctcccg ctcgaggtcg atcacgacgc gcaccgtatc ccggtcgaac cggcccgccc
     4561 ggacttgccg aagcaggccg ttgtggacaa cgaccggttg acggaggatc tcgtcccgga
     4621 tgccggccgc gcggatgtcg atgaagatcc ggggaggaag accgcgcgac ggatcggcgc
     4681 ggacggtgtt cgcctgccag gagacctctt cccccgtgtc gatcgccacg cgggtgtaga
     4741 tctcgttcgt ccaggcccgg atgtcggaga cgccgggagc ggcgtcggcg gccggcgggg
     4801 cgtcgaacgg aagacctgcg gcgcagagga agacggcgag gaacgccgcg cagaggtgtc
     4861 gccgcaactt tgtcaagtcg accctttccc gagcctctcc ttccattccg cgaggcgcat
     4921 caacgcgtcc agcggggaga gccgctccgg ttcgcaccgg cggatctggt ccaggaccgc
     4981 gttctcgtcc ccccgggcgc gccgggagaa cagctccatc tgggcgttgg cggattccgt
     5041 cacgacccgc gcgcccgcca gcgtcggaag cccgtactca ttatactcgg cggattcgag
     5101 gtttttcaaa atatcccggg cccggtccac cacggaggcc ggcaggccgg ccagccgcgc
     5161 cacctggatc ccgtacgact tgcttgcgct cccctcgtcg atccgccgca ggaagatgat
     5221 ctccccctgc cactcgcgca ccgccacgtg gaagttccgc gcgttcgcgc acgtcgacac
     5281 gatgtcggtc aactcgtgga agtgggtggc gaagaggacc tttgggcggg ccggggagtc
     5341 gtggaggtgc tccgccaccg cccaggcgat gctcaacccg tcgtaggtgc tggtcccgcg
     5401 ccccacctcg tccagaacca ccagcgtccg gtcggttacg ccgccgagga tccgcgccgt
     5461 ctcccgcatc tccaccatga aggtgctgtc cccccgggag agatcgtcgg aggcgccgat
     5521 gcgcgtgaag atccggtcca cgaggccgat ctccgcgcgg tccgcgggga cgaaggagcc
     5581 gacgtgcgcc agcagcacga tgagggccgc ctggcggata taggtggatt tccccgccat
     5641 gttcgggccg gtgaggacgg cgaggcgcgt cccttcgggc gagaggacgc agtcgttcgg
     5701 gacgaaggcg tggcgcccga ggatcttttc caccaccggg tggcgcccgt tctcgatcag
     5761 gatctccctt ccgccgttca ctcgcggacg cccgtagccg ttctccgccg cgagttccgc
     5821 gaacgagagg agcgcgtcga gctcggcgat cccgtccgcc gccgcgtaga ccgccgggag
     5881 ggctcctttc aacgaatccc gcagggcgag gaagagctcc tcctcccggg cggcgcggcc
     5941 ctcctgcgcg cgcagcaccc gcccctcgaa ctccttgagc tccggggtga tgtaccgctc
     6001 cgcgttcgcc agcgtctgct tgcggatgta gtcggcgggg accttttcga ggtgggcccg
     6061 ggtcacttcg atgtagtagc cgaagacgcg gttgtagccg accttgaggc cgccgatccc
     6121 tgttcgctga cgctcgcgcg cctccatctc cgccagcatc tccttgccgt gggtcaggag
     6181 atgcgtcagc tcgtcgacct gggtgtcgta ccccgggcgg aagacgcccc cctccttgta
     6241 tcccgcgggc ggcggatcga ccagcgccga ggagatccgg tcgaccaccg gggcgtggtc
     6301 cccgagaagt cccttcaccg accggaggcg catcgcggac ggctccccga gggcaatctt
     6361 gatcgcgggg atcgccgcaa ggtggtcgcg cagcgccgcc acgtcccggg ggccggaacg
     6421 gtcctgcgcc aggcgggagg cgagccgcga gagatccccc atcgcgtcga acggcggaga
     6481 aagggatttg cggatcgcgt gcgcctcgag gagctcaccc accgcgtcgt gccgctcccc
     6541 gatccgctcg acgtcgagca gcggcgcggc gagccaggcg cgcagcatgc gggcgcccat
     6601 cggggtccgc gtgcggtcca ccgcccagag gaggctcccc ttcctctccc ccgacatggt
     6661 ggagaagatc tccagcgtcc gcaccgcggt ctcgtccatc gcgaggtagc gccgcccttc
     6721 gcgctccgtc acccgcacga tctcggagag ggcggtcggc tggtgcagga acaggtagta
     6781 gaggaccgcc cggacgacgc cccccgacgg gtggtccgtg ggggggatcc cgccgatctc
     6841 ggggggaggc ggaaaaaccg cgacggcgga cggagagagg agcgtgagca gcttcccctc
     6901 gaggagccgc ttcccgcggg tggtggtcgg gttcccctcc ccctcgagcg cgacgaactc
     6961 cgcgggcgcg atccggaaca gggcgtccgc cagcgcctcc tcggtgtcgc acgtctcgtg
     7021 gaaaaactcg ccggtggtgg cgtcgagcgc ggcgcaggcg aacggggcga cgaaccggac
     7081 agcggcgagg aagttgtttc cccgggcgtc gaggcactcc tcgttgaaga ccagtcccgg
     7141 ggtgaccacc tcggtcacct cgcggcggaa gatccccttc tggccgggct cctcgatctg
     7201 ctcgcagatg gccaccttgc atccctgccg gatcagcttc gacaggtagg cgttgcgcgc
     7261 gtggtgcggc accccgcaca tcgggatgtt cgcttccttg tcgcgcgagg tcagggcgat
     7321 gtcgaggaga cgggaggcgc gcagggcatc ctcgaagaac atctcgtaga aatcgcccat
     7381 gcggaagaag aggatgcagt cccggtagcg ggacttgatc tccacgtact gccgcatcgc
     7441 gggggtcagc ggaaggctca tatcggatcg gggacgggga aggcccggcg tccggctacg
     7501 ggcgtaacag gccgaagacc gccgatagac ggcggcgggt ttcgaggagc gactcgggca
     7561 ggacgcgggt ttcctggacc ggggtcatga aattggtgtc gcccgcccag cggggaacga
     7621 ggtggacgtg gaggtggtcg acgatcccgg cgccggcggc cttgcccagg ttcatcccga
     7681 tgttcatccc ttccgttcga tattctaccg caagtgcccg tgtaccaatg gaaacaaggg
     7741 agaagagttc gtgcagctcc tcactcggca ggtcccacag gttcgccacg tgtcgacggg
     7801 gcgcgatcag cacatgtccg ttgttgtagg ggtagcggtt caggatcgcc acggtgtccg
     7861 ggaacagccc gacgacgagc cgctccgggt cttcgaggtc gccgtcccgg acacagaaaa
     7921 cgcacggggc cgtccccccg cccccgcccg cctggcggag atattccatc cgccacgggg
     7981 agaagatccg gtccaccgtc agccgttgac catttcccgc agttccttgc cgaccttgaa
     8041 gaaggggatc cgcttctcgg ggatggcgac cttctctccc gtcttcgggt tgcggccctc
     8101 cttcgcccgc cgggtccgca cggtgaagct cccgaacccg cggatctcga tcttttcgcc
     8161 acgatggagg gcgtccttca tgttgaggaa tacggtgtcg acgatgacct cacactcctt
     8221 cttggtcagg ttc
//
LOCUS       DASVJL010000171         1949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_401542, whole genome shotgun sequence.
ACCESSION   DASVJL010000171 DASVJL010000000
VERSION     DASVJL010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401542"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VF357_03490"
     CDS             complement(<1..1281)
                     /locus_tag="VF357_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7523241.1"
                     /translation="MNAPESPRHLGIAGRIAAAFIGSRLTPLFIIASVLLGVGAVLLL
                     PREEEPQIVVPMVDVFVQMPGASANEVENRVTRPMEKLLWEIPGVEYVYSTTSPGQSM
                     AIVRFKVGEDEEKSIVRLNQKMSANFDLIPPGATPPLVKPRSIDDVPILAVTLSSARH
                     DPFYLRRIASLLDDQVKSVPDVSEVRIIGGQRRQVRVLLDPVRMAGRGVAPAALVGML
                     RQANRQLPSGSIVTGNREILVETGGFLRTAEDVGQVVAGVMGGRPVYLRDVAQILDGP
                     EEPADYVFLGPGPAARASDPAADPAVTLSVAKRKGTNAIEVADKVLAKIETLKGTLVP
                     GDVTLTVTRNYGETAAEKSNELLLHMLIAVASVSILIWITLGLREAGIVATAIPVTLA
                     LTLTVFYLTGYTLNRVTLFALIFSIGILVDDAIVV"
     gene            complement(1278..>1949)
                     /locus_tag="VF357_03495"
     CDS             complement(1278..>1949)
                     /locus_tag="VF357_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7523242.1"
                     /translation="VAVKELERAQQKRAQVSAKIAQAKGQADAAKAMLAWTRVTAPFA
                     GVIVEKRADAGSMAIPGVPLFVLEDPRRQRIEMFVSETYLPLLGKGTPVQVVLDADPG
                     KPFSAVVTEIVPAIDPASRTFTLKADLPAGLARSGQAGKVRFAAGRGTVLAVPKRAIT
                     RAGGSDGVFTVGALDNVARLSMITLGAEFDDRVEVLSGIEDGARVALSPIDKLSDGAR
                     VEIRR"
BASE COUNT          314 a          690 c          655 g          290 t
ORIGIN      
        1 cacgacgatc gcgtcgtcca cgagaattcc gatggagaag atgagcgcga agagcgtcac
       61 ccggttcagc gtgtacccgg tgaggtagaa gacggtgagg gtgagggcga gggtcacggg
      121 gatggccgtc gcgacgatcc ctgcctcccg caacccaagg gtgatccaga tgaggatcga
      181 caccgaggcg acggcgatca gcatgtgaag gaggagctcg ttcgacttct ccgcggccgt
      241 ctcgccgtag ttccgcgtga ccgtcagggt gacgtcgccc gggacgaggg tccctttcag
      301 cgtctcgatc ttcgcgagga ccttgtccgc cacttcgatc gcgttggtcc ccttccgctt
      361 cgcgacggag agggtcaccg cggggtccgc cgcggggtcg ctcgcgcgcg ccgccggccc
      421 cggcccgagg aagacgtagt cggccggctc ctccggtccg tccaggatct gcgccacgtc
      481 ccgaagatag acgggccgcc cccccatgac gcctgccacc acctggccca cgtcctcggc
      541 cgttcgcagg aacccgcccg tctcgacgag gatctcccgg tttccggtga cgatgctccc
      601 cgagggaagc tggcggttcg cctgccgcag catcccgacg agagcggccg gggcgacgcc
      661 gcgccctgcc atccggaccg gatcgaggag cacccgcacc tggcgacgct gaccgccaat
      721 gatcctcacc tccgagacgt cggggaccga tttcacctgg tcgtcgagca gcgaagcgat
      781 ccgccgcagg tagaacgggt cgtggcgggc ggaggagagc gtgaccgcga ggatcgggac
      841 gtcgtcgatg gaacgcggct tgaccagggg aggagtcgcg ccggggggaa tcaggtcgaa
      901 attggcggac atcttctggt tcaggcggac gatgctcttc tcctcgtcct cgcccacctt
      961 gaaccggacg atcgccatcg actgcccggg cgacgtcgtg gagtagacgt actccacccc
     1021 cgggatctcc cacagaagct tctccatcgg ccgggtgacc cgattctcca cctcgttcgc
     1081 cgaggcgccg ggcatctgca caaagacgtc gaccatcggc accacgatct gcggctcctc
     1141 ctcccgcggg agaagaagca cggcgcccac gccgagcagg accgacgcga tgatgaacag
     1201 gggcgtgagt cgggacccga tgaaggcggc cgcgatccgc ccggcgatcc cgagatgacg
     1261 gggcgactcc ggggcgttca ccggcggatc tccacccgcg ccccgtcgga gagcttgtcg
     1321 atcggcgaca gggcgacccg cgccccgtct tcgatgccgg agaggacttc cacccggtcg
     1381 tcgaactccg cgccaagggt gatcatcgag aggcgcgcca cgttgtccag ggcgccgacc
     1441 gtgaacacgc cgtcggagcc tcccgcacgg gtgatggccc gtttcgggac cgcgagtacc
     1501 gtccccctcc ctgcggcgaa gcgcaccttg ccggcctggc ccgaccgggc caggcccgcc
     1561 ggaagatccg ccttcagggt aaaggtgcgg ctcgccgggt cgatcgcggg aacgatctcc
     1621 gtgacgacgg ccgaaaacgg cttcccgggg tcggcgtcca ggaccacctg gaccggcgtc
     1681 cccttcccga ggagcgggag atacgtctcc gagacgaaca tctcgatgcg ctggcggcgc
     1741 ggatcctcca ggacgaagag gggaacgccg ggaatggcca tcgaccccgc gtcggcccgc
     1801 ttctcgacga tcacccccgc gaagggggcg gtcacccgtg tccaggcgag catcgccttc
     1861 gccgcatcgg cctggccttt cgcctgggcg atcttcgcgc tcacctgcgc cctcttctgc
     1921 tgcgcccgct cgagttcctt caccgccac
//
LOCUS       DASVJL010000172         6236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_401621, whole genome shotgun sequence.
ACCESSION   DASVJL010000172 DASVJL010000000
VERSION     DASVJL010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401621"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VF357_03500"
     CDS             complement(<1..600)
                     /locus_tag="VF357_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7523243.1"
                     /translation="MQTFVDIAEVAPPRRLSARRSSGNPFLLRENDLELRLASSRSER
                     IEVQRLRFEVFTLELQLGLTSSLASGLDQDVHDGYCDHLLVVDTDRDCLVGTYRLLSF
                     DHVPSFGFYSESEFNLTNVKRSGLRLLELGRSCVALEYRDGRMISLLFRGIAEYLRRT
                     NADALMGCASIHGTDLPELASIQEMLCRRFMADPELRVTP"
     gene            834..2135
                     /locus_tag="VF357_03505"
     CDS             834..2135
                     /locus_tag="VF357_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX7523244.1"
                     /translation="MKRRTGPPPGLRKFLWGVATSAFQMEGAPRCDWTHWKVRDEGDR
                     QARLHGVGHLLRTDEDLALLPEIGVNAYRFSVEWSRVVPRRGEWDRKAMDRYVRIAAA
                     LRETGIEPVVTLHHFTNPSWLAKGTSWEDPSTVEEFLRFAERAVRSLRAHARVFITFN
                     EPNVFILGGYLRGIMPPGRKSIKGSFAAYANVFRAHGTLYDMIHTEGRERASVGVAHS
                     MVAFQPASEDSTMDKWAVKVAHAIYNMGLIETFRTGTLSVRLPSLFEEETAVGVRDKL
                     DFLGVNYYFRMFLRLSPLSMKGPEYFWEDRSKRGLTETGWEVYPKGFEDVLRAAALAE
                     VPLVVTENGTAETDDRRKIAFMKDHLRVVLRLMREGIDLRGYFWWSLMDNYEWLEGLR
                     PRFGLYRVDFETLERTPTASSAWFARWVKRRRALEGTGSSF"
     gene            2193..2903
                     /locus_tag="VF357_03510"
     CDS             2193..2903
                     /locus_tag="VF357_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523245.1"
                     /translation="MSMLLCDFHIHTTWSDGSVDLPEVVDIYGQAGFDVISITDHVYN
                     SDGTIGSWAERLNKTIAEETFPRYMETLERETERAMTRYGMLLIPGVEITKDYLSEEE
                     SAHVLLLDLRRYVSADLGWEEIFREARGQGALVVACHPHHTDHEIHDTLYFWNHRQKY
                     ASYFDAWEVANRTDLFSVVSLSNYPILANGDFHKANHLYSWKTLLPSLKNVEAVKECI
                     RDNRGVAITMFRNGTARE"
     gene            2941..4119
                     /locus_tag="VF357_03515"
     CDS             2941..4119
                     /locus_tag="VF357_03515"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7523246.1"
                     /translation="MNLLLEFPVAASLAACLLQYGSAWKALGKGRGAIDLADDGDLPA
                     VSILKPLKGVDDRLLDNLESFCRLDYPRYEIVFCVQGTSDPALRVARRVKETHPEREI
                     VVVVGDCQEGLNPKVNNMMPGYAAARYPFLLISDSNVAPAPGYLREAMAHFRDPSVGL
                     VSHLVRGIGAKTLGARLENQHLNTFILPSVSLLDRMFGMPCVVGKSMIMRRADLDAMG
                     GLSSVKDYLAEDYVLGEMFRKAGKKVVISGSPVDNVNVYRTLRQFLSRHARWNRMRFS
                     IAGAGYFAELFTNPVGLSLLMVAAAPGDAEAWTVAGGVAAAKMAMDYGMHRMLGDRVS
                     PGWVLLGPFRDVLAGGLWFSAFFSRNVEWRGRTLRITRGSRLVPVGEPAMEPATEAVR
                     "
     gene            4116..4343
                     /locus_tag="VF357_03520"
     CDS             4116..4343
                     /locus_tag="VF357_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523247.1"
                     /translation="MTRMRGSTSKDLGRRAFGTAGGWETSCDTPERSGTVIDLGEETL
                     RKPMALPISQAWKPRAGRIYNFPVRSFPDEK"
     gene            complement(4357..5241)
                     /locus_tag="VF357_03525"
     CDS             complement(4357..5241)
                     /locus_tag="VF357_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7523248.1"
                     /translation="MIDVIYNPVAGPRIVNRIDRVRSYLSARGLPFRIRETAAPGDAV
                     VMAREAALEGADAVVAVGGDGTMNEVADGLAGSATRLAFVPHGTGNVFAREFSLPKSV
                     EGCLDLLSSGKTISVRMAKANDRRFVLLASAGFDAEVVERMNPRQKNLLGIAAYALCG
                     ARHLLRSQPTLWLEFPDRERVEAQAVIVVRGKKYGGNVTIAPEGDIARDTLQVIALLR
                     KGRWSIAKFALDALRGKHTASRHVLVRESLSLFVRSAIPSACQVDGEYLGPLPVRFTV
                     TDALLRVVVPKGFPFPPA"
     gene            complement(5295..5456)
                     /locus_tag="VF357_03530"
     CDS             complement(5295..5456)
                     /locus_tag="VF357_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523249.1"
                     /translation="MVRQGKKIPLSAVREQVRASLRNDKQRQALDDYIAELKKKARIT
                     IDNAVLSKV"
     gene            complement(5475..>6236)
                     /locus_tag="VF357_03535"
     CDS             complement(5475..>6236)
                     /locus_tag="VF357_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523250.1"
                     /translation="AWMGDPIGSIAMTYIPVDKKKKALDKLIVGWLLAQDARSRGMDN
                     TPEFREFFQRSERRVRVYALFRKEIGAKLKTDDKAVKAEIARIQETNKKISAEDAAQR
                     ASFRISEGQVRKIQEELIATAKKETGATVNQATMGNIAKGEGVPDNAVIASAGEEKIL
                     YADVKKILEGRSPTGESQGRQDLSKNPEVIAKVVDQELNVLVLSAYARKQGIEGSDEY
                     KMTRQEEERNILRTLVGQATVRKEVTITDKEVENA"
BASE COUNT         1076 a         1941 c         2046 g         1173 t
ORIGIN      
        1 gggagtgacc cgtagttctg ggtcggccat gaaccgccgg cagagcattt cctggatcga
       61 ggccagttcc ggaagatcgg tcccgtggat gctcgcgcac cccatcaatg cgtcggcgtt
      121 ggtccggcgc aagtactccg cgattccccg gaacagcagg gagatcattc tcccgtcccg
      181 gtattccagc gccacgcagc ttcgccccag ctccagcagg cgcagccccg accgcttcac
      241 gttcgtcagg ttgaactcgg attcggagta gaagccgaac gacggcacgt gatcgaagga
      301 aaggaggcgg tacgttccca cgaggcaatc gcgatcggtg tcgacgacga ggaggtggtc
      361 gcagtagccg tcgtgcacgt cctggtccag accgctggcc agggaggagg tcagcccgag
      421 ctgaagttcc agggtgaaga cctcgaagcg gagcctctgg acctcgatcc gttcggaccg
      481 gcttgatgca aggcggagct ccagatcgtt ttcccggagc aggaacgggt ttcccgacga
      541 tcggcgggca gagagtcgcc ttgggggagc gacctccgcg atgtcgacaa atgtttgcat
      601 ggcattccct ccttgggatc cccgcgcgtt ccacccggtg gcgggtcgtt gttggataag
      661 cggagaatac cggcatggta tgaagatcgc attacggccg ggtaatgatc gtgcgtcggg
      721 gaatcgccgg tgggaggttt aacctcctct taatacgaaa atgaaacgcg ccggagtagg
      781 atgatcctct ggatacggtc gtcgtatcgg ccggcccatg gagggacgcg gtgttgaaga
      841 ggagaacggg ccccccgccc gggttgagga agtttctgtg gggagtggcg acctccgcgt
      901 tccagatgga aggcgcgccg cggtgcgact ggacccactg gaaggtgagg gacgagggcg
      961 accggcaggc gaggctgcac ggcgtggggc atctcttgcg cacggacgag gacctggctc
     1021 tcctgccgga aatcggcgtc aacgcctacc ggttttccgt ggagtggagc cgggtggtcc
     1081 cccgccgcgg ggagtgggac cggaaggcga tggaccgcta cgtccggatt gcggccgcgc
     1141 ttcgggagac cgggatcgag ccggtggtca ccctgcacca tttcaccaac ccctcgtggc
     1201 ttgcgaaggg gacctcctgg gaggatccgt ccacggtgga agagttcctc cggttcgcgg
     1261 agcgggcggt acggtccctc cgtgcgcacg cgcgggtctt catcaccttc aacgagccga
     1321 acgtcttcat tctcggcggg tacctccgcg gaatcatgcc gccagggaga aagagcatca
     1381 agggaagctt cgctgcgtac gcgaacgtct tccgcgccca cggcacgctc tacgacatga
     1441 tccacacgga gggacgggag cgggcgtcgg tgggcgtggc gcacagcatg gtcgcctttc
     1501 agccggcctc ggaagactcc acgatggaca agtgggcggt caaagtcgcc cacgcgatct
     1561 acaacatggg cctgatcgag acgtttcgca ccggcacgct ttccgtccgg cttccctccc
     1621 tcttcgagga ggaaacggcc gtgggtgtcc gcgacaagct ggatttcctc ggggtgaact
     1681 actacttccg gatgttcctg cgcctgtccc ccctgagcat gaagggaccg gaatacttct
     1741 gggaggatcg ctcgaagcgc ggtctcacgg agaccgggtg ggaggtctat ccgaaagggt
     1801 tcgaagacgt gctgcgggcc gccgccctgg ccgaggtccc ccttgtcgtc acggaaaacg
     1861 gcaccgcgga aaccgacgac cgccgcaaga tcgccttcat gaaggaccat cttcgggtcg
     1921 tcctccggct gatgcgggag gggatcgacc tccggggata tttctggtgg tccctgatgg
     1981 acaactacga gtggctcgag gggttgaggc cccggttcgg gttgtaccgt gtcgacttcg
     2041 agactttgga gcgcactccc accgcctcct ccgcctggtt cgcccgttgg gtgaagcgga
     2101 gaagagcgct ggaggggacc ggcagctcct tttaacatcc acttcacacg cgcttcatcc
     2161 ccccgtagtc ccaccccgtt atattcctcg tcatgtccat gctgttgtgc gacttccaca
     2221 tccacaccac ctggtcggac ggatcggtcg atctcccgga agtggtggac atctacgggc
     2281 aggcgggatt cgacgtcatc tccatcaccg accatgtcta caacagtgac ggcacgatcg
     2341 gctcgtgggc cgagcgattg aacaagacga tcgcggagga gacctttccg cgctacatgg
     2401 agaccctcga gcgtgagacc gaacgggcga tgacgcggta cggcatgctg ctgatccccg
     2461 gggtggagat cacgaaagat tacctttccg aggaagagtc tgcccatgtc ctgctcctgg
     2521 acctgaggag gtacgtctcc gccgacctgg gctgggagga gatctttcgc gaagcgcggg
     2581 ggcagggggc gctcgtggtg gcatgccacc cccatcatac ggaccacgag atccacgaca
     2641 ccctctactt ctggaaccac cggcagaagt acgcctcgta tttcgacgca tgggaagtgg
     2701 cgaatcggac cgacctgttc tccgtggtca gcctgagcaa ctacccgatc ctcgccaacg
     2761 gcgacttcca caaggcgaat cacctttatt cctggaagac gctcctgccg agcctgaaga
     2821 acgtcgaggc ggtgaaggag tgcatccggg acaaccgggg ggtggcgatc accatgttcc
     2881 gcaacgggac ggcgagggag tgagggagag cacgatcttg cacccgcaag ggaggttgcg
     2941 atgaacctgc tgctcgaatt tccggtagcc gcgtcgcttg cggcgtgcct cctgcaatac
     3001 ggctccgcgt ggaaagccct cgggaagggt cgcggggcga tcgaccttgc cgacgatgga
     3061 gacttgccgg ccgtttcgat cctcaagcct ctcaaggggg tggacgaccg gctcctcgac
     3121 aacctggaga gcttctgccg gctggactat ccccgctacg agatcgtctt ctgcgtccag
     3181 gggacgagcg acccggcgct gcgggtggcc cggagggtga aggagacgca ccccgaacgg
     3241 gagatcgtcg tggtggtggg tgattgccag gagggcttga atcccaaggt gaacaacatg
     3301 atgccggggt acgccgccgc gaggtacccg ttccttctca tcagcgacag caacgtggcg
     3361 cccgcgccgg ggtacctgcg ggaggcgatg gcacacttcc gggacccctc ggtgggactg
     3421 gtgagccacc tggtccgcgg aatcggcgcg aagacgctgg gggcgcgact ggaaaaccag
     3481 cacctgaaca cgttcatcct cccctccgtc tccctcctcg accggatgtt cggcatgcct
     3541 tgcgtcgtcg gcaagtcgat gatcatgcgc cgcgcggacc tggacgcgat gggcgggttg
     3601 tcctccgtga aagattatct cgccgaggat tacgttctcg gggagatgtt ccggaaggcc
     3661 gggaagaagg tggtgatctc gggatcaccc gtcgacaacg tgaacgtgta ccgcaccctg
     3721 cgtcagttcc tctcgcgaca tgcccggtgg aaccggatgc gcttttcgat cgcgggagcg
     3781 ggatatttcg ccgagctgtt caccaacccg gtgggacttt cgctgctgat ggtcgccgcg
     3841 gctccggggg atgccgaggc gtggacagtc gccggcgggg tcgcggccgc caagatggcg
     3901 atggattacg gcatgcatcg gatgctgggg gaccgcgttt ccccggggtg ggttcttctc
     3961 ggccccttcc gcgacgtgct ggccggaggg ctatggttct ccgccttctt ctcgcgaaac
     4021 gtcgagtggc gcggaagaac gttacggatt acccgcgggt cccggttggt gcccgtgggc
     4081 gagccggcga tggaacccgc tacggaggcg gtgcgatgac ccgcatgcga ggaagcacgt
     4141 cgaaagacct cggacgccgc gctttcggta cggcgggtgg atgggaaacg tcctgcgata
     4201 cgccggagcg gtccgggacg gtcatcgacc tgggggaaga gaccctgcgc aagccgatgg
     4261 ccctgccgat atcgcaggcg tggaagcccc gcgcaggccg catctacaac tttcccgtga
     4321 gaagcttccc ggacgaaaag taacgccgta cctttgtcac gccgggggga aggggaaccc
     4381 cttcgggacg acgacccgca ggagggcgtc ggtcaccgtg aaccggaccg ggagcggccc
     4441 gaggtactcg ccgtccacct ggcaggcgga cgggatcgcg ctgcggacga agagggaaag
     4501 gctctcccgg accaggacgt ggcgggaggc ggtgtgcttc ccgcgcaagg cgtcgagggc
     4561 gaacttcgcg atcgaccagc gcccctttcg caggagggcg atcacctgga gcgtatcccg
     4621 cgcgatgtcc ccttccggcg cgatcgtcac gttcccgccg tactttttcc cgcggacgac
     4681 gatgaccgcc tgcgcctcga cccgctcgcg gtcggggaac tcgagccaga gggtcggctg
     4741 ggagcggagg aggtgccgcg cgccgcacag ggcgtaggcg gcgatcccga gaaggttctt
     4801 ctggcgtgga ttcatccgtt ccaccacctc cgcatcgaac cccgcggagg ccagcaggac
     4861 gaagcgccgg tcgttcgcct tcgccatccg gacggagatc gtcttcccgg aggagagcag
     4921 gtcgaggcac ccttccacgg attttgggag ggaaaattcg cgggcgaaga cgttcccggt
     4981 cccgtggggg acgaaggcca ggcgggtggc gcttcccgcc aacccgtccg cgacctcgtt
     5041 catcgtaccg tccccgccga cggcgaccac ggcgtcggcc ccttcgagcg ccgcctcgcg
     5101 cgccatcacg acggcatccc cgggggcggc cgtctcccgg atccggaagg ggagaccgcg
     5161 cgccgacagg tacgagcgga cccggtcgat ccggttcacg atcctggggc cggcgacggg
     5221 attgtagatc acgtcgatca ttctaaccga ttataggata aatcgggcga aaccgtaccg
     5281 aacgcttctc cgggttacac cttggacagg acggcgttgt cgatcgtgat cctggccttc
     5341 ttcttcagtt cggcgatata gtcatcgagt gcctggcgtt gtttgtcgtt ccggagggac
     5401 gcccggacct gctccctgac ggcggagagg ggaatcttct tcccttgccg taccatttcc
     5461 ctggagcgct ccgcttaggc gttctcgacc tccttatccg ttatcgtaac ctccttgcgg
     5521 acggttgctt ggccaaccag cgtcctgaga atgttccgct cctcctcttg acgtgtcatc
     5581 ttgtattcgt cggaaccttc gatcccctgt tttcttgcgt acgccgaaag caccagtacg
     5641 ttcaactcct ggtcgacgac cttcgcgatc acctcggggt tcttcgaaag gtcctgccgg
     5701 ccttgggatt ccccggtcgg cgacctcccc tcgaggatct tcttgacgtc agcgtagagg
     5761 attttctcct ccccggcgga ggcgatgacg gcgttgtccg ggacaccctc ccccttcgcg
     5821 atgttcccca tcgtcgcctg gttgacggtg gccccggtct ccttcttcgc cgtggcgatc
     5881 aactcctcct ggatcttgcg gacctgtccc tcggaaatcc taaacgatgc gcgttgcgcg
     5941 gcgtcctctg cggagatctt cttgttcgtc tcctgtattc tggcaatctc cgccttgact
     6001 gccttgtcgt cggttttcag cttcgccccg atttccttcc ggaaaagggc atagacccgg
     6061 acccggcgct cgcttctttg gaaaaattcc ctgaactccg gcgtattgtc catgccccgg
     6121 gagcgggcgt cctgcgccag aagccaccca acgatcagtt tatcgagtgc ctttttcttt
     6181 ttgtccaccg ggatgtacgt catagcgatc gacccgatgg gatcacccat ccacgc
//
LOCUS       DASVJL010000173         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_682543, whole genome shotgun sequence.
ACCESSION   DASVJL010000173 DASVJL010000000
VERSION     DASVJL010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682543"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VF357_03540"
     CDS             complement(<1..995)
                     /locus_tag="VF357_03540"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX7523251.1"
                     /translation="MSVPQDIRRISDTVWEIPVSHKAGMLVPARIFATETLLSDFDDG
                     VFEQITNVATLPGIVGHACCMPDGHWGYGFPIGGLAAMDPDHGVISPGGIGFDINCGM
                     RLCLTNLTYGEVQPRLRNLVDRMAGRVPAGVGAEGTVRLSRDEFRDAVEQGARWAVKR
                     GFGWKEDLERTEEGGCLPGADASRVSEKAVDRGYRQIGTLGSGNHYLEIQVARAENFF
                     DQETAGIFGIVLPDQVVVMFHCGSRGFGHQVATDYLRVFVAAMRGKYGIHVPDRELAC
                     APFRSPEGQDYFAAMKCAVNMSFVNRQVILHRIREVFSEVFGKDPKDLGMRQVYDV"
BASE COUNT          277 a          498 c          491 g          254 t
ORIGIN      
        1 acgtcgtaga cctggcgcat cccgaggtcc ttcgggtctt tcccgaacac ctcggagaac
       61 acctcgcgga tccggtggag gatgacctgg cggttgacga aggacatgtt gacggcgcac
      121 ttcatcgcag caaagtagtc ctgcccctcc ggggagcgga aaggggcgca cgccagctcc
      181 cgatcgggga catggatgcc atacttcccc cgcatcgccg cgacgaagac ccggaggtaa
      241 tcggtcgcca cctgatggcc gaacccccgg ctgccgcagt ggaacatcac gaccacctgg
      301 tcggggagta cgatgccgaa gattcctgcg gtttcctgat cgaagaagtt ttcggccctg
      361 gcgacctgga tctccaggta gtggttcccc gaccccaagg tgccgatctg ccggtacccc
      421 cggtcgaccg ctttctcgct cacccgcgag gcgtcggcgc cgggaaggca ccccccttcc
      481 tcggtgcgct cgagatcctc cttccagccg aacccgcgct tcaccgccca ccgggccccc
      541 tgttccacgg catcgcgaaa ctcgtcccgg gagagccgga cggtcccctc ggcgcccacg
      601 ccggcgggaa cgcgcccggc catgcggtcc acgaggttcc ggaggcgcgg ctggacctcc
      661 ccgtacgtca agttcgtgag gcagagccgc atcccgcagt tgatgtcgaa gccgattcca
      721 ccgggggaga tcaccccgtg atcggggtcc atcgcggcga ggccgccgat ggggaaaccg
      781 tatccccaat ggccatcggg catgcagcag gcgtgaccca cgatgcccgg gagggtggcg
      841 acgttcgtga tctgttcgaa gacgccgtcg tcgaaatccg aaaggagcgt ctccgtggcg
      901 aagatgcggg ccggtacgag catccccgcc ttgtgggaga cggggatctc ccagaccgta
      961 tcggaaatcc gccggatatc ctgcggcacg gacacggggc gcctccctcc gtcgactttc
     1021 tattcattat atcccgacgc ataccggcct gcttcggagg cggcttcatc ttcgcgtgat
     1081 accccttcct cgctacgttc cggcagccgt ccctggctgc cttcactgta taaaatggga
     1141 gcacgtagtg cacgacacag gcgtgtccct tacggacagg atatccgcca tcccttgccg
     1201 taccttccgt gggatcccct cccgagcggg catctgacct tggcggagcc aggacggcgg
     1261 ggggcaaggg aggtgccagc gaaccggaca gggacgtccg gtgagcgtag tgagggaggg
     1321 tgatcccatg gaagggaagg cagaatcggc ggaatttaaa ggaagccccc gccccagtac
     1381 gagctccgcg ccgctggggt acccccgctg taggaaaaac ggggggaagg agcccggagt
     1441 ctgggtaacc aacgggcata tgttgccgga ttgatgaatt gaagcactga ggcgaggtag
     1501 ccgtggctct ggaccggatc
//
LOCUS       DASVJL010000174         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_495915, whole genome shotgun sequence.
ACCESSION   DASVJL010000174 DASVJL010000000
VERSION     DASVJL010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495915"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VF357_03545"
     CDS             complement(<1..534)
                     /locus_tag="VF357_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEX7523252.1"
                     /translation="MADPGFDTVLRSVAGRGGGDAELCVTRMRDRRYEAREGRLDGIA
                     LADTVALGLRVFRAGRMGFSYGFRGDAADLARMVEEALFCAEASDPDAAYGLPDAAGP
                     ATDLPLYDPSVETVPEGEKGEFVRILEAHALARDPRMKRVRAAALRETVATVSLFHSR
                     GFAATRRESLCSAHVETV"
     gene            complement(527..997)
                     /locus_tag="VF357_03550"
     CDS             complement(527..997)
                     /locus_tag="VF357_03550"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX7523253.1"
                     /translation="MIRKARMSDVKGIHQLIAEYAQKGDMLPRSLADIYENLRDYFVF
                     LGDDGELVGSAAIHIMWEDLAEVRSLAVREGKMRRGVGTQLVESCISEAIVLGIGRVF
                     ALTYKPEFFEKLGFHVVDKAELPQKIWTDCLKCSKFPDCDEVALVADFSGRRHG"
     gene            complement(994..>1674)
                     /locus_tag="VF357_03555"
     CDS             complement(994..>1674)
                     /locus_tag="VF357_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523254.1"
                     /translation="SLAGALEEEARLRKELLKEEEGGVAAAKKLSATRRKAAAAMGPA
                     VEKELARVALAGARFRAEVSSREAVPAALSASGIDEAELLFSANPGQEVRPLAQTASG
                     GELSRVMLSLRNAASRGGAGKTLVFDEIDTGIGGQVAERVGARLKSLSVAAQVVCVTH
                     LPQVAAFADHHLQVVKKAAAGTVATGVKPLSKQDRIGELARMISGAEITEKAKAHAKT
                     LIEKAAGE"
BASE COUNT          261 a          646 c          503 g          264 t
ORIGIN      
        1 caccgtctcc acgtgcgccg aacagaggga ctcccgcctc gtcgccgcga acccccggga
       61 atggaacagg gacacggtgg cgaccgtctc gcgcagcgcc gccgcgcgca cgcgcttcat
      121 ccggggatcc cgggcgaggg cgtgcgcctc gagaatgcgg acgaactccc ctttctcccc
      181 ttccggaacc gtttcgacgg aggggtcgta gagcggcaga tccgtcgccg gtcccgccgc
      241 gtccggcaag ccgtacgcgg catccgggtc ggaagcttcc gcgcagaaaa gcgcctcttc
      301 caccatccgg gccaggtccg cggcgtcacc ccgaaacccg taggagaacc ccatccgccc
      361 ggcgcgaaag acgcgcaacc cgagggccac ggtgtccgcg agcgcgatcc cgtcgagccg
      421 cccttcgcgc gcctcgtacc ggcgatcccg catgcgcgtc acgcacaact ccgcgtcgcc
      481 ccctccccgc cccgcaacgg agcgcaggac ggtatcgaac ccgggatcag ccatgccgcc
      541 tccccgagaa gtcggcgacc agcgcaactt catcgcagtc ggggaacttg gagcacttca
      601 ggcaatcggt ccagatcttc tgcgggagtt cggccttgtc cacgacgtga aacccgagct
      661 tctcgaagaa ctccggcttg tacgtcagcg cgaagacgcg cccgatcccg agcacgatcg
      721 cctcggagat gcacgactcc accagctgcg tcccgacgcc gcggcgcatc ttcccttcgc
      781 gaacggcgag ggagcgcacc tccgccaagt cctcccacat gatgtggatc gccgcggagc
      841 cgaccagttc cccgtcgtcc cccaggaaga cgaagtaatc ccgcaggttt tcgtagatgt
      901 cggccaggga ccgcggcagc atgtcgccct tctgcgcgta ttcggcgatc aactggtgga
      961 tccccttgac gtccgacatc ctcgctttcc ggatcattcc cccgccgcct tctcgatcag
     1021 cgtcttcgcg tgcgccttcg ctttttccgt gatctccgcg ccggagatca tccgggccaa
     1081 ttctcctatc ctatcctgtt tcgacagcgg tttcacgccg gtggcgaccg ttcccgccgc
     1141 cgccttcttc acgacctgga gatggtggtc ggcgaaggcc gcgacctgcg gcaggtgcgt
     1201 gacgcagacg acctgcgcgg cgacggaaag gcttttcaac cgcgccccga cgcgttcggc
     1261 cacctggccc ccgatccccg tatcgatctc gtcgaacacg agcgtctttc cggcgccccc
     1321 ccgggaagcg gcgttccgca gggaaagcat cacgcgggag agctccccgc ccgaggcggt
     1381 ctgcgcgagc ggtcgaactt cctggcccgg gttcgcggag aagaggagct ccgcctcgtc
     1441 gatccccgac gccgaaagcg ccgcggggac cgcctcgcgg gaggaaacct ccgcccggaa
     1501 tcgcgccccg gcgagggcga cccgcgcgag ctccttttcc accgccggtc ccatcgccgc
     1561 cgccgccttc cggcgcgtcg cggagagttt cttcgccgcc gcgaccccgc cctcctcttc
     1621 cttgaggagc tcttttcgaa gccgcgcctc ctcttcaagc gccccggcca gcga
//
LOCUS       DASVJL010000175         8104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_964745, whole genome shotgun sequence.
ACCESSION   DASVJL010000175 DASVJL010000000
VERSION     DASVJL010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964745"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VF357_03560"
     CDS             <1..455
                     /locus_tag="VF357_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX7523255.1"
                     /translation="YGGSDQDALRFSVSHSNRRALYDINRGREIGVDLEKIRPEFSFE
                     EISDKYFSAREAAFLRTRPIHDRKEIFFTYWTLKEAFMKGTGKGFSLPLNRFEITHDP
                     PKVVSLQGDSEAAARWSLRNMHLEPGYASALAVEGPDLPIRCWKLQLR"
     gene            472..1128
                     /gene="tenA"
                     /locus_tag="VF357_03565"
     CDS             472..1128
                     /gene="tenA"
                     /locus_tag="VF357_03565"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEX7523256.1"
                     /translation="MHSFYREMREKTDPLWEAIFHHPFVKGIGDGTLSRDRFEFYLKQ
                     DYVYLIDFSRVFALASAKSWTLADMGTFADLLHATLNTEMELHRKTCAAFGIAEQELA
                     KTRKSPLTSAYTDLLVRTAYEGGLSDILAVLLPCACGYVEIGKRLKSEGLPEDRFYRD
                     WIDTYSSPEFQEFADWLIGRMNEHAADAPAQRKEYWHRLYESSARYEYLFFDMSWKKE
                     "
     gene            1143..2180
                     /gene="thiL"
                     /locus_tag="VF357_03570"
     CDS             1143..2180
                     /gene="thiL"
                     /locus_tag="VF357_03570"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX7523257.1"
                     /translation="MGKLRKSVGEIGEFPLIDRIRNILPVSRNKDLLVDIGDDTAVVR
                     MDERRALLLTCDIQVEGRHFRFDRTTPYRVGRRAMAVNLSDIASMGGKPTYALVSLGL
                     PADFPVESYDRLFEGMRDELLPHRAYIIGGNLARTKDGLIVDVTLLGEADLSRVLTRS
                     GARVGDRIFVTGHLGASGAGFQALKAYGKKVPGKYKEMVNRHILPVPRVDLGRRISRS
                     GVASAMIDLSDGVAGDLYHVCTRSGVGAEIHEDLLPLPERIGEIAVKSGKSVLELALH
                     SGEDYELLFTVPPGVSARRIRSLPGGSGIPVTEIGRIVKREEGYCLVDSRGKKRPLTP
                     AGWDHFRRTRG"
     gene            2197..2994
                     /gene="thiD"
                     /locus_tag="VF357_03575"
     CDS             2197..2994
                     /gene="thiD"
                     /locus_tag="VF357_03575"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX7523258.1"
                     /translation="MKRALTIAGSDSGGGAGIQADLKTFMAFGVHGMSAITALTAQNT
                     VGVQGIFEVSPEFVWKQIESVMTDIGADAAKTGMLSNARIVKTVAEAIRTFRIPNLVV
                     DPVMVAKSGDSLLAEDARQAIRDELLPLATVITPNVFEAEALLERRIEDLDSMRNAAQ
                     ELKKFGCRWVVIKGGSLDIESRAVDVVCDGTEPILLASPRMASRNTQGTGCTFASAIA
                     AGLAKGLSPLEAIKRAKEYITEAIRSGPPIGSGHGPANHLTGVHSKW"
     gene            3099..3683
                     /locus_tag="VF357_03580"
     CDS             3099..3683
                     /locus_tag="VF357_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7523259.1"
                     /translation="MRRVVYDSLKCTACKTCELQCSVSHSLSKDLLAAIFESPPPLPR
                     MSVAWTPGVNVPVKCAHCETAPCLLGCPMGAIRRDRGSDAVLIDEDRCIGCFSCVLLC
                     PYGAVRLSFDRKRAYKCDGCADRISEGLEPACASSCPTGALAWREVDEVAREKTALAA
                     VRDAAALARGGELAAGEGSPLGTILKMREEMARG"
     gene            3676..5607
                     /gene="cooS"
                     /locus_tag="VF357_03585"
     CDS             3676..5607
                     /gene="cooS"
                     /locus_tag="VF357_03585"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HEX7523260.1"
                     /translation="MDKTIERTAYANPGDIELTRKAADDNVELVTDRLRKMMPECGFG
                     ELGTCCVMCYMGPCRIDPFGQGTRTGVCGATPDVIVARNFLRSATGGASSHAGHAREL
                     AILLLEIAEGKAPGYKIREEGKLLSLADKLGVPVDGRPVNAVAADVARKALEDFGRQD
                     GEPMNWIRARASRMEYAKWEKLGLIVSNPHNEIETAMHRTSMGNDADPLNLWVATLRM
                     GMVDNFAGLMLATDLSDVIFGIPTPKVVTANYSVLKEGEVNIACHGHNPILASKVAEW
                     ADRLEGEAKAAGAGRVNVVGICCIGNELVTRYGIPYAGHEAQTELFLVTGAIDAMVVD
                     MQCIWPSLPAIAKCYKTRFITTVPFVRHPEAIHVDFRPEEADERAQEIVRHAIAAYKE
                     RKAAGIRPRIPNVSSKLLTGISVESLVSVLSTVDAKDPLKPVIDAIATGDILGAVGMV
                     GCPNPKLRDGAMSEKMAEGLLRNNVLIVTTGCVNHILAQGGFLSSDATEKYAGPRLKK
                     VLTAIGQAAGLGGPLPPVLHMGSCVDNSRIYDLLAALAGKLGVEISQLPVAGSAPEFI
                     TEKAISIGSFFLAAGILVHLAPAPRVFGSPFAVSLLTEKLPGLNGGKVLVAATPDAAV
                     SGILAHLREKRQALGLPQA"
     gene            5627..6871
                     /locus_tag="VF357_03590"
     CDS             5627..6871
                     /locus_tag="VF357_03590"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7523261.1"
                     /translation="MERVLIVGNGYAGARASEVLGSLGGFEVLHVSDEAYPAYCRHLL
                     PGLAAGERAVEDLFLPPVNGNGRGSGARSGIAVSRLLPNEKRALLSNGEEVLFDHALI
                     ATGARVFVPSSLRHIFGRCGNVIAMRQMKEALSLRRILEGGKRRVAIIGAGRIGVLLA
                     EALKRAGVSISLIDIAPEILPTMLHGGVAARLRPHLEAEGDLSVLAGSSVASAETEGE
                     TVRALHLSDGTVVPCDAVVLATGVVPNTEFLEGEGSDRTDGLPVDARMETSAPGIYAA
                     GDVIRFQTATGKTAPGQLILNARLQAEVAARNIAGERATCPPLFVGNVVKIGPVIGAV
                     AGEVDGTGTEDYRVGGSFLRVTLEGGAMTGFQFVGLPEDLRGLVPGVLKRFEATWVRE
                     ALATCGGPGFSPRVLAGGGAWV"
     gene            6862..7626
                     /locus_tag="VF357_03595"
     CDS             6862..7626
                     /locus_tag="VF357_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX7523262.1"
                     /translation="MGLKVAVAGKGGAGKTTVCALLAGVLSRAGHRILLVDLDSDPNL
                     SSALGFPADAAQPLVQRKELIAERTGSTGEPGGMFLLNPRVSDIAEKFALKRTEDVFL
                     LPVGTIEMAGEGCFCPQAAFVKALVRRLILDEKESLLLDLEAGLESFGRSVVEGLDLL
                     LIVVEPGMRSLDTARRILAMASSLGVRAIRVVANKVRPENLETLRVRMRENGVRLDLT
                     LPYREEFARRDLDGAGIFDFTEPTFEADALRLLQAV"
     gene            complement(7654..>8104)
                     /locus_tag="VF357_03600"
     CDS             complement(7654..>8104)
                     /locus_tag="VF357_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX7523263.1"
                     /translation="CWLLLRGIKTLSVRMTRAQENAEALARFLSEHPAVGKIYFPGLP
                     NHPRRELHFSQASGPGSIISFDLKREKAVPAFLSALKTILLAESLGGVESLITHPSTM
                     THADIPLEEQAAVGLTPSLIRLSVGIEDRGELQADLSQALRKVLPSR"
BASE COUNT         1427 a         2479 c         2803 g         1395 t
ORIGIN      
        1 tgtatggtgg atccgatcag gatgccctcc ggtttagcgt ctcgcattcc aaccggcgcg
       61 ccctgtatga catcaaccgg ggacgggaaa tcggtgttga tcttgaaaaa attcgtccgg
      121 aattttcgtt tgaggagatc agcgataaat atttttccgc acgggaagcc gcctttctcc
      181 gaacgcgccc tatacatgat cggaaagaaa tcttttttac ctattggacg ctgaaagaag
      241 cgttcatgaa gggaacgggc aaagggttct cgcttccgtt gaatcggttc gaaataacgc
      301 atgatcctcc gaaagtggta agcctccagg gagattctga agcggctgca cgatggtccc
      361 tgcggaatat gcatcttgag cctggatatg cctcggccct tgccgtggag gggcccgatt
      421 taccaattcg atgttggaag ctgcaacttc gctgagacgg ggcatgaact gatgcattcg
      481 ttttaccggg aaatgcggga aaagaccgat ccgttgtggg aggcgatttt ccatcacccc
      541 tttgtgaaag ggatcggaga cggcaccctg tcgcgggacc ggttcgagtt ctatctcaag
      601 caggattacg tgtacctgat cgatttcagc cgggtcttcg cgctggccag cgcgaaatcc
      661 tggacgctgg cggacatggg aaccttcgcc gacttgctgc acgccacgct gaacacggaa
      721 atggagctcc atcggaaaac atgcgccgcc ttcgggatcg cggagcagga actggcaaag
      781 acccgcaaaa gtccgctcac gtccgcctac accgatcttc tggtgcggac cgcttatgag
      841 ggcggattat ccgacatatt ggcggtgttg ctgccgtgcg cctgcgggta cgtggagatc
      901 gggaagaggc tcaaatcgga ggggcttccg gaggaccggt tttaccggga ttggatcgac
      961 acctactcct ccccggaatt tcaggaattt gccgattggc tcatcggcag gatgaatgaa
     1021 catgccgccg acgctcccgc gcaacgcaag gagtactggc atcgactgta cgaatcgagc
     1081 gcccgctacg aatacctgtt cttcgacatg agctggaaaa aggagtaggg agggcagggg
     1141 cgttggggaa acttcggaaa agcgtgggag agatcgggga gttccccctg atcgaccgga
     1201 tcaggaacat ccttcccgta tcccggaaca aggatctgct ggtcgacatc ggcgacgaca
     1261 ccgcggtggt ccggatggac gaacgtcggg cgctgctgtt gacctgcgac atccaggtgg
     1321 aaggccgcca cttccggttc gatcgaacaa cgccctaccg tgtgggtcgc cgcgccatgg
     1381 cggtgaatct cagcgacatc gcctccatgg gagggaagcc cacgtacgcg ttggtttcgc
     1441 tggggctccc tgcggatttt cccgtggaaa gttacgaccg gttgttcgaa gggatgcggg
     1501 acgaattgct gccccaccgg gcctacatca tcggggggaa cctggcccgg accaaagacg
     1561 gcctgatcgt ggacgtcacg ctgttgggcg aggccgatct ttcccgcgtg ctcacccgca
     1621 gtggggcgcg ggtcggcgac cgcatcttcg tgaccgggca tctgggggcc tccggcgcgg
     1681 gtttccaggc gctgaaggcg tacgggaaaa aagtccccgg aaaatacaag gagatggtga
     1741 atcgccacat cttgccggtc cctcgggtgg atctgggacg gcggatctcc cggtcgggcg
     1801 tcgcctcggc catgatcgac ctgagcgatg gagtggccgg cgacctgtac cacgtgtgca
     1861 cccggagcgg ggtgggggcg gaaatccacg aagatttact gccgctgccg gaacgtatcg
     1921 gagagatcgc ggtgaaatcc ggcaagtcgg tcctggaact tgccttgcac agcggggagg
     1981 attacgaact gctcttcacc gttccccccg gggtttcggc gaggaggatt cgatccctcc
     2041 ccgggggttc ggggattccg gtcacggaaa tcgggaggat cgtgaagcgg gaggaagggt
     2101 actgcctggt ggactcccgc gggaagaaaa gaccgctgac tccggccggc tgggaccatt
     2161 tccgacggac gcggggatga aggagggaga agcggaatga aaagggcgct gaccatcgcc
     2221 ggctccgaca gcgggggagg cgccggcatc caggcggacc tgaagacgtt catggccttc
     2281 ggcgttcacg ggatgtccgc catcaccgcc ctcacggccc agaacacggt gggcgtgcag
     2341 gggatcttcg aggtttcccc cgaattcgtc tggaagcaga tcgaaagcgt catgaccgac
     2401 atcggggccg atgccgccaa gaccgggatg ctgtccaatg cgcggatcgt aaagacggtg
     2461 gcggaagcga tccggacctt ccgcatcccc aacctggtcg tcgacccggt gatggtcgcc
     2521 aagagcggag actccctcct ggcggaggat gcccgccagg cgatccgcga cgagctgctg
     2581 ccgctggcca cggtcatcac tcccaacgtg ttcgaggccg aagcgcttct ggagcgcagg
     2641 atcgaagatc tggactccat gcggaacgcg gcgcaggaac tgaaaaaatt cgggtgtcga
     2701 tgggtggtca tcaagggagg aagcctggat atcgaatccc gggcggtcga cgtggtctgc
     2761 gacgggacgg aaccgattct gctggcgtcg ccccggatgg catccaggaa cacccagggc
     2821 accggctgca cgttcgcctc cgccatagcg gccgggctcg ccaaggggct ttccccgctg
     2881 gaagccatca agcgcgccaa ggagtacatc actgaagcga tccgaagcgg acctccgatc
     2941 ggaagcggcc atggtcccgc caaccatctg acgggcgtgc acagcaaatg gtgaggcggc
     3001 ttgacaaggg agtgcgccgg gagaagactg tttcgggtac acagtggctg ccgcgcatcg
     3061 cgctttcttc gcttccactt cgacgggaag gagtgcccat gcgaagagtc gtttacgact
     3121 ccttgaagtg caccgcctgc aagacgtgcg aactccagtg ttccgtttcc cactccctct
     3181 cgaaagacct gctcgccgcc atcttcgaat cgccgccgcc gctcccccgc atgtccgtgg
     3241 catggacgcc gggggtgaac gtgccggtca agtgcgcgca ctgcgaaacc gcgccgtgcc
     3301 tcctcggctg tcccatgggc gccattcgga gggaccgtgg aagcgacgcc gtcctgatcg
     3361 acgaggaccg ttgcatcggg tgcttctcct gcgttctcct ctgcccgtac ggggcggttc
     3421 gcctctcgtt cgaccggaag cgcgcgtaca agtgcgacgg ttgcgcggac cggatctccg
     3481 aaggcctgga accggcgtgc gcctcctcct gtccgaccgg ggccctcgcc tggcgggaag
     3541 tcgatgaggt ggcgcgggag aagaccgcgc tggccgcggt gcgggatgcc gcggccctgg
     3601 cgcgcggcgg cgaactggcg gccggggagg gttcccccct cggcacgatc ctcaaaatga
     3661 gggaggagat ggcccgtgga taagacgatc gaacggaccg catacgcgaa ccccggcgac
     3721 atcgaactga cccgcaaggc ggcggacgac aacgtcgaac tcgtcacgga ccgccttcgg
     3781 aagatgatgc ccgagtgcgg gttcggcgag ctggggacgt gctgcgtaat gtgctacatg
     3841 gggccgtgcc ggatcgaccc gttcggccag gggacccgca cgggcgtttg cggggcgacc
     3901 cccgacgtca tcgtggcgcg gaacttcctc cggtccgcca cgggaggggc ctcttcccac
     3961 gcgggccacg cgcgggagct ggcgatcctg cttctcgaga tcgccgaggg gaaggcgccc
     4021 gggtacaaga tccgggagga gggaaaactc ctttctctcg cggataagct cggggtgccc
     4081 gtcgacgggc ggccggtcaa cgcggtggcg gcggacgtcg cccggaaggc gctcgaggat
     4141 ttcggccgac aggacgggga gccgatgaac tggatccgcg cccgggcttc caggatggag
     4201 tacgcgaagt gggaaaaatt gggactcatc gtctccaacc cccacaacga aatcgagacg
     4261 gcgatgcatc gtacgtcgat ggggaacgat gcggacccgc tgaacctgtg ggtcgccacc
     4321 ctccggatgg ggatggtcga caacttcgcg gggctgatgc tggccaccga tctctccgac
     4381 gtcatcttcg ggatccccac gcccaaagtg gtcacggcga actattcggt tctgaaggaa
     4441 ggggaggtga acatcgcctg ccatgggcac aatccgatcc tcgcctcgaa ggtggcggaa
     4501 tgggccgacc ggctcgaggg ggaggcgaag gcggccggcg ccggacgggt gaacgtggtg
     4561 gggatctgct gcatcgggaa cgagctggtg acgcggtacg gcatccccta cgccgggcac
     4621 gaggcgcaga cggagctctt cctcgtcaca ggagccattg atgcgatggt ggtcgacatg
     4681 cagtgcatct ggccgtccct gcccgcgatc gcgaaatgct acaagacccg tttcatcaca
     4741 accgtcccct tcgtccggca tccggaggcg atccacgtcg atttccggcc cgaggaggcg
     4801 gacgagcgcg cgcaggagat cgtccgccat gccatcgcgg cgtacaagga acggaaggcg
     4861 gccgggatcc gtccccggat ccccaacgtt tcttcgaagc tactgacggg catctccgtc
     4921 gagtcgctgg tctccgtcct ctccaccgtg gacgcgaagg acccgctgaa gccggtcatc
     4981 gacgcgatcg cgaccgggga tatcctgggg gcggtgggga tggtcgggtg cccgaacccg
     5041 aagctgcggg acggagcgat gtccgagaag atggccgagg ggctgctccg gaacaacgtc
     5101 ctcatcgtga ccaccgggtg cgtgaaccat atcctcgcgc agggcgggtt cctgtcgagc
     5161 gacgccaccg aaaagtacgc cgggccgagg ttgaagaagg tgctgactgc gatcgggcaa
     5221 gcggcgggac tggggggtcc cctgccgccg gtcctccaca tggggtcgtg cgtcgacaac
     5281 tcgaggatct acgatctgct cgccgcgctg gcagggaaac tgggcgtcga gatcagccag
     5341 ctcccggtgg ccggctccgc gccggagttc atcaccgaga aggcgatctc catcgggagc
     5401 ttcttcctgg ccgccgggat cctggttcac ctggcgcccg cgccgagggt ctttgggagc
     5461 cccttcgccg tctcgctcct gacggagaag ctcccgggac tgaacggggg gaaggtgctc
     5521 gtggccgcga cgcccgatgc cgcggtctcg gggatcctcg cccatctccg ggagaagcgg
     5581 caggcgctgg gcctccccca ggcttgaccg gagcgggaga aaccggatgg aacgggttct
     5641 gatcgtcggg aacggctacg ccggcgcccg cgcgtcggag gtcctcggtt ccctcggggg
     5701 cttcgaggtg ctccacgtct ccgacgaggc gtatcccgcc tattgccgac acctccttcc
     5761 cgggcttgcg gcgggcgagc gggccgtgga ggacctgttc ctcccgccgg tgaacggaaa
     5821 cggtcgaggc tccggcgccc ggtccgggat cgcggtctcc cgcctcttgc cgaacgagaa
     5881 gagggcgctc ctttcgaacg gggaggaggt tctcttcgat catgcgctga tcgcgacggg
     5941 ggcgcgggtc ttcgtccctt cgagcctccg gcacattttc ggaagatgcg gcaacgtgat
     6001 cgccatgcgg cagatgaagg aagcgttgtc gctgcgccgg atcctcgaag gagggaaacg
     6061 gcgggtcgcg atcatcgggg cggggaggat cggcgtcctc ctcgcggagg cgctcaaacg
     6121 ggccggggtg tccatttccc tgatcgacat cgcccccgag atccttccca caatgctcca
     6181 cggcggcgtc gcggcgcggc tgcggcccca cctcgaggcg gagggagact tgtccgtgct
     6241 tgcggggagc tccgttgcga gcgcggagac ggaaggggaa acggtccgtg ccctgcacct
     6301 ctccgacgga accgtcgtgc cgtgcgacgc cgtggtcctg gccaccggcg tggtccccaa
     6361 tacggagttc ctcgaagggg agggatccga ccggaccgac gggctcccag tggacgcccg
     6421 gatggagacg tcggcgcccg ggatctacgc cgcgggggac gtgatccgct ttcagaccgc
     6481 gacggggaaa acggcgccgg gccagttgat cctgaacgcg cgcctccagg ccgaagtggc
     6541 cgcacggaac atcgcggggg agcgggcgac atgcccgccg ctgttcgtgg ggaacgtcgt
     6601 gaagatcggc ccggtgatcg gcgccgtggc cggcgaggtc gatggaacgg ggaccgagga
     6661 ttaccgtgtc gggggcagct tcctccgcgt cacgctggaa gggggtgcga tgacggggtt
     6721 ccagttcgtc ggccttcccg aggaccttcg ggggctggtc cccggggtcc tcaagcgctt
     6781 cgaggcgacg tgggtccggg aggcccttgc cacctgcggc ggtccgggat tttccccacg
     6841 ggtgcttgca gggggaggcg cgtgggtctg aaggtcgccg tcgcgggaaa ggggggcgca
     6901 ggcaagacga ccgtctgcgc gctcctggcc ggggtcctct cccgcgcggg gcaccggatc
     6961 ctgctggtgg atctcgattc cgatccgaac ctatcctccg ccctcggatt ccccgccgac
     7021 gcggcccagc cgctcgtcca acgcaaggag ttgatcgcgg aacggaccgg gtccacgggg
     7081 gagcccgggg gaatgtttct cctcaacccc cgggtgtccg atatcgcgga gaagttcgcc
     7141 ctgaagcgga cggaggacgt cttcctcctt cccgtcggga ccatcgagat ggccggggag
     7201 ggatgctttt gtccgcaggc ggcgttcgtc aaggcgctcg tccggcggct cattctcgac
     7261 gaaaaggagt ccctcctcct cgacctcgag gcggggctcg aatcgttcgg ccgctcggtc
     7321 gtcgaggggc tcgatctcct tttgatcgta gtcgagccgg ggatgcgctc cctggacacg
     7381 gcgcggagga tcctcgcgat ggcctcctcc ctcggggtgc gcgcgatccg ggtcgtcgcg
     7441 aacaaggtcc gcccggaaaa tctcgaaacc ctccgggtcc ggatgcgcga gaacggcgtc
     7501 cgcctggatc tgacgcttcc gtaccgcgag gagtttgcgc gcagggacct cgatggagcc
     7561 gggatcttcg acttcacgga gccgacgttc gaggcggatg cccttcggct gctccaggcc
     7621 gtctgatcct ccccttcgca tcccgaaaaa ccctcagcgg cttggcagga ctttccgcaa
     7681 ggcttgggag aggtcggcct gcaattcccc ccggtcttcg attcccacgg agagccggat
     7741 cagggaaggg gtgagcccga ccgcggcctg ctcctcgagg ggaatgtccg cgtgggtcat
     7801 cgtcgaagga tgcgtgatga gcgattcgac cccgccgagg ctctccgcaa gaaggatggt
     7861 tttcaaggcg ctcaggaagg ccgggaccgc cttctcccgc ttcaggtcga acgagatgat
     7921 cgagccgggc cccgaggcct gggagaaatg cagctccctc cgggggtggt tcggcaggcc
     7981 cgggaaatag attttcccga cggcggggtg ttccgacagg aaccttgcca gggcttccgc
     8041 gttctcctgg gcgcgcgtca tcctgacgga caaggtcttg atcccccgca ggaggagcca
     8101 gcag
//
LOCUS       DASVJL010000176         6863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_683040, whole genome shotgun sequence.
ACCESSION   DASVJL010000176 DASVJL010000000
VERSION     DASVJL010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683040"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VF357_03605"
     CDS             complement(<1..524)
                     /locus_tag="VF357_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX7523264.1"
                     /translation="MPEAYICDAVRTPIGRYAGCLSAIRTDDLAATPVVELMKRNPSV
                     DWGGVNDVMYGCTNQAGEDNRNVARMAGLLAGLPPEVPGVTFNRLCGSGMDAVGSAAR
                     AIRVGEAELIVAGGVESMSRAPYVMNKGAAPFDRGVEMHDSTIGWRFVNRRMKERYGV
                     ESMASTAENVADHFR"
     gene            complement(559..2880)
                     /locus_tag="VF357_03610"
     CDS             complement(559..2880)
                     /locus_tag="VF357_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7523265.1"
                     /translation="MIREKVARLSARMHDGPQYPSQGAVLFVDLERREHFSKYLPVGV
                     LRSFLSGRGANMFLLHNLLLDGREPLDPQIPMIFGSGVFTGTLPSAARGNLTSVAPDS
                     DAILDSNCGDFFPAFLKLNGYDHLVLYGQAAGWTLLELSGKEVRTHDATPYLGMDNTD
                     FTRAVEMDFSCTERKDMAMARITRAGENLVLCSGIMGGPKAIYARCGTGAKMGSLRLK
                     AVMILGRGEPPDLPPAYKENNRDLARKILSTSVVKYALKKVGTPFLYKPSRVLGAIGT
                     KNNQETSWYDDLDADNFDVYRPGMDGCYQCPIRCRPLNDLTPEGKGGWGAGALKGVTG
                     NAGYDEGQAGVEHRHEKSYRGIRGDGTFDRHDKGDGPDYVTLGKMGPMIGIREPEQVL
                     RLNNILNDLGLDSASTGSAIAWAMELYQRGIITAKETGGLDLTWGNYEVIEKLLYMTS
                     RREGFGDVIADSARAVERGRYPEEALKYRMAVKGLFQSDPHDARIIKGFALGLAVSTR
                     GMDHLRNRPTLEINAKINDDREFKTALYGGTVAPEPTSYEGKEHAVATCEKMFAVGDA
                     VGICRFATKLFNSPSTADYKDFALQLKELTGEEFTPAQLDEIGRNITGIERLINARLG
                     LTERDDTLPDRWFEEEITAGPFKGEKIDRAAFEALKIRYYDLLGLNGAGVPELEWHRR
                     LAEAITGFAVQVTLPEGIPGAPEGAVIVDQPVSDVAGLWEALKKLLPHAARKLDDSSL
                     IVSVNGTMVLSNEDATPVRGGDEVTVMRIIAGG"
     gene            complement(2877..3818)
                     /locus_tag="VF357_03615"
     CDS             complement(2877..3818)
                     /locus_tag="VF357_03615"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX7523266.1"
                     /translation="MGKSSYQPKRFAVVGAGPVGAIVAAFLAKGGCDVTLCDVVPSLL
                     EPALDPGILVEGTDTLQVKVARTTTRVDDLVGDPPDVVIVAVKATALPLIASTLEGFA
                     AGGRYVVSWQNGIDTERVLAKHLGAEFVMRAVVNLGCVPVGPAHVRIAFHHRPHYIQE
                     LDPRSMDAAVGISEVFTACGLDTLHTDQIQHMVWRKAVLNACMNPICAVTGKTMAEVI
                     NDPILFHLVDALIKEGVSVARANEFRLGSNFYPYCIDYIRNAGHHKPSMLQDIEAGRR
                     TEVDYINGKIVEYGAQAGVPAPYNTMIRGLVKALEPK"
     gene            complement(3822..4262)
                     /locus_tag="VF357_03620"
     CDS             complement(3822..4262)
                     /locus_tag="VF357_03620"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523267.1"
                     /translation="MKLKQLSIFLENSPGRLYEATHALGEAGINLRSLCISDSADFGV
                     LRILVSDVAKARRVIMEKQLPARIDDVVAVEIDDTPGSLARILDPFKEKRVNVEYMYA
                     LAGASSGKAVMVFRFSDNDKAIEILRANKIRILDAEAFGMIEGK"
     gene            complement(4307..5653)
                     /locus_tag="VF357_03625"
     CDS             complement(4307..5653)
                     /locus_tag="VF357_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX7523268.1"
                     /translation="MAWSKEETIGREEMQRIQLGKWQKMVSYVHAKNPVYRKKMDEAG
                     VKPGEIRSLDDVRRLPFTTKIEIRDYYPFGLFTAAKEDIVEFHATSGTTGKMVVIGYT
                     RNDVELWTEVMARACTGAGITKNDIVQNIYGYGLFTGGLGTHYGAIRVGASVIPISGG
                     NTQKQIMLMQDFGSTVVTSTPSFLMRLHEVGMEMGVDLKKLKLRIGLLGAEPWSETMR
                     QSIDEKFGIKACDMYGLSEMIGPGVSFECHEMRNGLHVNEDYFYPEIIHPDTCEVLPY
                     GEEGELVITNFANEGQPLMRYRTRDISRLTVGPCACGRTLVKMQRVTGRTDDMLIIRG
                     VNFFPSQIESIIMKRWGVSPHYLVVVERPGAMDEVEVKIEVNDEFMKKAAADVLAGSE
                     KDILGDVATAKKKKGNLQRDIKDMIGITVKVTLVAPGTIPRSEGKAKRVDDRRPKG"
     gene            complement(5705..6571)
                     /locus_tag="VF357_03630"
     CDS             complement(5705..6571)
                     /locus_tag="VF357_03630"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7523269.1"
                     /translation="MNVEEIRTVGVVGAGSMGTGIIQVAAQSGFEVKAVDVDDAACAR
                     AAKAIAKILERLVAKEKITADQMKETLGRISFSTDVNTLKDVPFIFEAVFEAVAVKKE
                     FFAKLDAVAWDDTVYATNTSSISITEMASLVKRPAKFVGMHFFNPVPVMKLVEVIPAL
                     QTEQATIDLALAMAKKLGKTAITCKDTPGFVVNRLLVPYMLDAVRLLEAGVASAEDID
                     TAMKLGTGMPMGPFELMDYTGVEISYYVGEIFHDYTKEARFAPPGLLRNMVKAGYLGR
                     KTGKGFYDYPAK"
     gene            complement(6612..>6863)
                     /locus_tag="VF357_03635"
     CDS             complement(6612..>6863)
                     /locus_tag="VF357_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7523270.1"
                     /translation="LVDRLIEPEIFMEEVMKFAEQLASGAGRAMGYIKAAVYEGIDLP
                     MDQALAVERKYSLENLGTHDAKEGLVAFAEKRKPNFLSR"
BASE COUNT         1069 a         2302 c         2128 g         1364 t
ORIGIN      
        1 cggaaatggt ccgccacgtt ctccgccgtc gacgccatcg actcgacccc gtaccgctcc
       61 ttcatccgcc ggttcacgaa gcgccacccg atcgtgctgt cgtgcatctc gaccccccgg
      121 tcgaacggag cggccccttt attcatcacg taaggggcgc gggacatgct ctcgaccccg
      181 cccgcgacga tcaactccgc ctcccccacg cggatcgcgc gggccgcact cccgaccgcg
      241 tccatcccgg agccgcacag gcggttgaac gtcacccccg ggacctccgg cgggaggccg
      301 gcgaggagcc ccgccatccg cgccacgttc cgattatcct ctcccgcctg gttcgtgcac
      361 ccgtacatca cgtcgtttac ccctccccaa tcgacggagg ggttccgctt catgagctcc
      421 acgaccggtg tcgcggcgag gtcgtcggtc cggatcgccg acaggcagcc ggcgtacctt
      481 ccgatcgggg tgcgcaccgc gtcacagata taggcctcgg gcatatgcgt cctcctgact
      541 ggattcgatc gccggcgctc accctcccgc gatgatgcgc atgacggtga cctcgtcgcc
      601 gccgcggaca ggggtcgcgt cttcgttcga aaggaccatg gtgccgttga cggaaacgat
      661 cagggagctg tcgtccagtt tccgtgccgc gtggggaagc agcttcttca gcgcctccca
      721 aagtccggcg acgtcggaca cgggctgatc gacgatgacc gccccttccg gggctcccgg
      781 gatcccctcg ggaagcgtga cctggacggc gaatcccgtg atcgcctccg cgagccggcg
      841 gtgccactcg agctccggga cgccggcccc gttcaggccg aggaggtcgt agtaacggat
      901 cttcagcgcc tcgaacgcgg cccggtcgat cttctcccct ttgaacggcc ccgcggtgat
      961 ctcctcctcg aaccatcggt cggggagcgt gtcgtccctc tccgtcagtc cgaggcgggc
     1021 attgatcagg cgctcgatcc cggtgatgtt ccgtccgatc tcgtccaact gcgcaggggt
     1081 gaactcctcc cccgtcagct ccttcagctg cagggcgaag tccttgtagt cggccgtcga
     1141 gggggagttg aacagcttcg tcgcgaagcg gcagatgccg acggcgtccc cgaccgcgaa
     1201 catcttttcg cacgtggcga cggcgtgctc cttcccttcg tagctcgtcg gctcgggggc
     1261 gaccgttccg ccgtagaggg cggtcttgaa ttcgcggtcg tcgttgatct tcgcgttgat
     1321 ctcaagggtg ggccggttcc ggagatgatc catgccgcgg gtcgagacgg cgaggccgag
     1381 ggcgaaccct ttgatgatgc gcgcgtcgtg cggatcggac tggaacagcc ccttcacggc
     1441 catccggtac ttcagcgcct cttccggata ccttccccgc tccaccgccc gggcggagtc
     1501 ggcgatcacg tcgccgaatc cttcccgccg ggacgtcatg tacaggagct tttcgatgac
     1561 ctcgtagttc ccccaggtca ggtcgagccc gccggtctcc ttcgccgtga tgatgccgcg
     1621 ctggtagagc tccatcgccc aggcgatcgc gcttcccgtg ctggccgagt ccagcccgag
     1681 atcgttgaga atgttgttca gacgcaggac ctgttccggc tcccggatcc cgatcatcgg
     1741 gcccatcttg cccaaggtga cgtaatcggg gccgtccccc ttgtcgtggc ggtcgaaggt
     1801 tccgtcgccc cggatccccc ggtagctctt ctcatgacga tgctcgaccc ccgcctggcc
     1861 ttcgtcgtac ccggcgttcc cggtcactcc cttgagagcg cccgcgcccc accccccctt
     1921 cccttccggg gtgaggtcgt tcaacgggcg gcagcggatc ggacactggt agcagccgtc
     1981 catgccgggc cggtagacgt cgaagttgtc ggcgtcgagg tcgtcgtacc agcttgtctc
     2041 ctggttgttt ttcgtcccta tcgctccgag gacgcggctg ggcttgtaga gaaaaggggt
     2101 gcccaccttt ttcagggcgt acttgacgac gctggtcgac aggatcttcc gcgcgagatc
     2161 ccggttgttc tccttgtagg ccggaggtag gtcgggcggc tctccccgac caagaatcat
     2221 cacggctttc agccggagag agcccatctt cgcgccggtg ccgcaccggg cgtagatcgc
     2281 cttcggcccc cccatgatcc cggagcacag gaccaggttc tcgccggcgc gcgtaatgcg
     2341 ggccatcgcc atgtccttcc gttcagtgca ggagaaatcc atttcgacgg cccgggtgaa
     2401 gtcggtgttg tccatcccga gatagggagt ggcgtcgtgg gtgcgaacct ccttgccgga
     2461 gagctcgagg agggtccatc cggcggcctg tccgtagagg acaaggtggt cgtacccgtt
     2521 cagcttgaga aacgcgggga agaaatcccc gcagttgctg tcgaggatcg cgtcgctgtc
     2581 gggagcgacg ctggtgaggt tgccgcgggc ggccgagggg agcgtcccgg tgaagacgcc
     2641 gctgccgaaa atcatcggta tctgcgggtc gagcggctcc ctcccgtcga gcaggaggtt
     2701 atggaggagg aacatgttgg cgccgcggcc ggagaggaac gaccgcagca ccccgacggg
     2761 aaggtacttc gagaaatgct cccgccgctc gaggtcgacg aacagcaccg ctccctgcga
     2821 cggatactgc gggccgtcgt gcatccgcgc cgagagccgc gcaacttttt cccggatcat
     2881 ttcggttcga gcgccttgac gaggccgcgg atcatcgtgt tgtacggggc ggggacgccg
     2941 gcctgtgcgc cgtactcgac gatcttgcca ttgatgtagt cgacctcggt ccgccggccc
     3001 gcctcgatgt cctggagcat ggacggcttg tggtggccgg cgttccggat atagtcgatg
     3061 cagtacggat agaagttcga gccgagccgg aactcgttcg cccttgcgac ggagaccccc
     3121 tccttgatga gggcatcgac caggtggaag aggatcggat cgttgatgac ctcggccatc
     3181 gtcttcccgg tgacggcgca gatcgggttc atgcaggcgt tcagcaccgc cttgcgccag
     3241 accatgtgct ggatctggtc ggtgtggagg gtgtcgagcc cgcacgcggt gaagacttcg
     3301 gagatcccga cggcggcgtc catcgaccgg ggatcgagct cctggatgta gtggggacga
     3361 tggtggaagg cgatccggac gtgggccggc cccaccggga cgcagccgag gttgacgacg
     3421 gcccgcatga cgaattcggc gcccaggtgt ttcgcgagga cgcgctcggt gtcgatgccg
     3481 ttctgccagc tgacgacgta cctccccccg gcggcgaacc cctcgagggt ggaggcgatc
     3541 agcgggaggg cggtggcctt taccgccacg atcacgacgt cgggaggatc cccgaccagg
     3601 tcgtcgacgc gtgtggtcgt cctggccacc ttcacctgga gggtgtcggt cccctcgacg
     3661 aggatgccgg gatcgagggc cggttcgagg agcgacggca ccacgtcgca aagggtgaca
     3721 tcgcaccctc ccttcgcgag gaaagcggcg acgatcgcgc cgaccgggcc ggcgccgacg
     3781 accgcgaacc gcttcggctg gtagctgctc tttcccatcg gctacttgcc ctcgatcatt
     3841 ccaaacgcct cggcatcgag tatccggatc ttgttcgccc gaaggatttc gatcgccttg
     3901 tcgttgtcgc tgaaccggaa gaccatcacc gccttcccgg acgaggcgcc cgccagggcg
     3961 tacatgtatt cgacgttcac cctcttttcc ttgaacgggt caaggatccg ggccagggaa
     4021 ccgggagtgt catcgatctc caccgcgacg acgtcgtcga tgcgcgccgg cagctgtttt
     4081 tccatgatga cccttcgggc cttcgccacg tcggacacga ggatccgcag gactccgaag
     4141 tccgcgctgt cgctgatgca aagggaccgc aggttgatcc cggcctcgcc cagcgcatgg
     4201 gtcgcctcgt acagccgccc cggcgagttc tccaggaaga tcgacagctg cttcaacttc
     4261 atcggaacct cccgtgcgta acgtaatgcg ctccgctgag cgcgcgttaa cctttcgggc
     4321 gccggtcgtc gacgcgcttc gccttcccct cgctgcgcgg gatcgtcccg ggcgcgacga
     4381 gcgtcacctt cacggtgatc ccgatcatgt ccttgatgtc gcgctggagg ttccccttct
     4441 tcttcttcgc ggtcgccacg tcgcccagga tgtccttctc gctccccgcg aggacgtcgg
     4501 cggcggcttt cttcatgaac tcgtcgttca cctcgatctt gacctcgacc tcgtccatcg
     4561 ctcccggacg ctcgacgacg accaggtaat gcggcgacac gccccaccgc ttcatgatga
     4621 tcgactcgat ctgggacggg aagaagttca cgccgcggat gatcagcatg tcgtcggtcc
     4681 ggcccgtcac ccgctgcatc ttgacgaggg tccgtccgca ggcgcacggg ccgacggtca
     4741 gccgcgagat gtcccgggtg cggtaccgca tcagcggctg gccttcgttg gcgaagttcg
     4801 tgatgacgag ctccccttcc tcaccgtatg gaagcacctc gcaggtgtcg ggatggatga
     4861 tctcggggta gaagtaatcc tcgttgacgt ggagaccgtt tcgcatctca tggcactcga
     4921 acgagacccc cgggccgatc atctcggaga ggccgtacat gtcgcacgcc ttgatcccga
     4981 atttctcgtc gatcgactgc cgcatcgtct cgctccacgg ctcggcgccg agcaggccga
     5041 tccggagctt gagctttttc aggtcgaccc ccatctccat cccgacctcg tggagtcgca
     5101 tgaggaacga cggggtggag gtgacgaccg tgctgccgaa atcctgcatc agcatgatct
     5161 gcttctgcgt gttccccccg gagatcggga tcaccgacgc ccccacccgg atcgccccgt
     5221 aatgggtccc gaggcccccg gtgaagagtc cgtagccgta gatgttctgg acgatgtcgt
     5281 ttttcgtgat gccggccccg gtgcaggccc gggccatcac ctcggtccac agctccacgt
     5341 cgttccgcgt gtatccgatg acgaccattt tcccggtcgt tccggacgtg gcgtggaact
     5401 cgacgatgtc ctccttggcg gcggtgaaga ggccgaacgg atagtagtcg cggatctcga
     5461 tcttcgtggt gaacggcagt ctccggacgt cgtccagcga gcggatctcc cccggcttca
     5521 ccccggcttc gtccatcttc ttacggtaga cggggttctt cgcgtggacg taggaaacca
     5581 tcttctgcca cttcccaagc tggatccgct gcatctcctc gcgaccgatc gtctcttcct
     5641 tgctccaggc catggcgtcg tctcttcctt tcgctcggtg ggttgcgaat tcgtcggaaa
     5701 ccgcctactt cgccggatag tcgtagaaac ctttgccggt cttccgcccg aggtacccgg
     5761 ccttcaccat gttccgcagc agcccgggag gggcgaaccg ggcctccttc gtgtaatcgt
     5821 ggaagatctc cccaacataa taggagattt ccacgccggt gtagtccatg agttcgaagg
     5881 gacccatcgg catcccggtc ccaagcttca tcgccgtgtc gatatcctcg gccgacgcga
     5941 cgcccgcctc gagaagacgg accgcgtcga gcatgtacgg cacgagaagc cggttcacga
     6001 cgaacccggg ggtgtccttg caggtgatcg cggtcttgcc cagcttcttc gccatcgcca
     6061 gcgccaggtc gatggtcgcc tgttcggtct gcagggcggg gatcacctcg accagcttca
     6121 tcaccggaac cgggttgaag aagtgcatgc cgacgaactt cgcggggcgc ttcacgaggg
     6181 aggccatctc ggtgatcgag atggaggagg tgttcgtcgc gtagacggtg tcgtcccacg
     6241 ccacggcgtc gagtttcgcg aaaaactcct tcttcacggc gaccgcctcg aagacggcct
     6301 cgaagatgaa gggaacgtct ttcagcgtgt tcacgtcggt ggaaaacgag atcctcccga
     6361 gggtctcctt catctggtcc gcagtgatct tctccttggc gaccagccgt tcgaggatct
     6421 ttgcgatcgc cttcgccgcc cttgcgcagg ccgcgtcgtc gacgtccacc gccttgactt
     6481 cgaagcccga ctgggccgcg acctggatga tccccgtccc catgctgccc gcgcccacga
     6541 cccctacggt cctgatctcc tcgacattca tcgcgcgtcc tccgttggaa tggtatacgt
     6601 cccttttccg gttatctgct caggaaattc ggcttccgtt tttcggcgaa ggcgacgagc
     6661 ccttccttcg cgtcgtgggt cccgaggttt tcgaggctgt atttccgttc gaccgccagc
     6721 gcctggtcca tgggaaggtc gatcccctcg tacaccgccg ccttgatgta ccccatcgcc
     6781 ctccctgctc cggacgccaa ctgttccgcg aatttcatca cttcttccat gaaaatctcc
     6841 ggctcgatca gccggtcgac gag
//
LOCUS       DASVJL010000177         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1528350, whole genome shotgun sequence.
ACCESSION   DASVJL010000177 DASVJL010000000
VERSION     DASVJL010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528350"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VF357_03640"
     CDS             <1..518
                     /locus_tag="VF357_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7523271.1"
                     /translation="ADGLEVLRAIRSDDTISRTPVVMLTAKGDETDRVIGLELGADDY
                     IPKPFSPREVVARIKAILRRSRPGAREPEPATLTYRELRMDVARHEVRCQGKPVSLTS
                     KEFRILQALLSSSGRVLSRDAILSKVWGEDTHVIDRTVDVHIAKLRQKIPFLVKDIET
                     VKDVGYKLRES"
     gene            525..2333
                     /locus_tag="VF357_03645"
     CDS             525..2333
                     /locus_tag="VF357_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7523272.1"
                     /translation="MARRLSIAAKFFLTHLVIAGIALLIAGAVGFFLVRNLVMADADE
                     NLLARARIVAETFRPLLADSPQNRERIAGEGDRLGKEIGARITVVLPDGAVVADSTVG
                     AAGVPGMENHAHHPEIRDALSGTTGVSLRRSITVREEQRYAALPITIGGTIVGAARAS
                     VPVATLTRRLWQITTIIWGTGFAALLLILGGTAVMARRITIPLAEMQAAAKEMGAGNL
                     GRRVQIRTGDELEEMANAMNRMASHLTDTIGQLDAGKARLETLLANLDDGVIVIAADR
                     SVRMMNRDAGKIFDASETMGAGRPYPEVIRHPEALAFIDGWIKGEEPKPRDVSILTRH
                     GSRMVRCSGTTVRYLGESNADVLLTLRDVTEERRLAQVKSDFVSNASHELRTPLTNIR
                     GYLEAIQDAVREGTPPAPSFVAVAHANALRMERLIDDLLELSRAESTGVPLEWEEVPL
                     QDFLSRVAEFHRLPAERAGKTLVVVAGEGTLRADVPKLALAMSNLVDNAIKYGKEGGR
                     VTVSGKIEPSGCTLEVKDDGPGIPPEDIPRIFERFYRVDKGRSRELGGTGLGLSIVKH
                     IVESHEGILQAESRLGVGTRFVIRIPGTPGSLSTPP"
BASE COUNT          431 a          803 c          823 g          360 t
ORIGIN      
        1 aagccgacgg gctggaagtg cttcgggcaa tccggtcgga cgatacgatc agcagaacgc
       61 cggtggtgat gctcaccgcg aagggggacg agacggacag ggtcatcggg ctggaactgg
      121 gcgcggacga ctacatcccc aaaccgttca gccccaggga ggtcgtggcc cgcatcaagg
      181 cgatcctgcg ccgcagtcgt cccggagccc gggaacccga gccggcaacg ttgacttacc
      241 gggaactgcg gatggacgtg gcccgtcacg aggtccgatg ccaggggaag ccggtctccc
      301 tcacttccaa ggagttccgc atcctccagg cgctcctctc ctcttcgggg cgagtcctct
      361 cccgcgatgc gatcctctcg aaggtctggg gggaggacac ccacgtgatc gaccgcacgg
      421 tcgacgtcca catcgcgaaa cttcgccaga agatcccctt cctggtcaag gacatcgaga
      481 cggtcaagga cgtcggctac aaactgcggg aaagctgacc ccgcttggcc cgccgcctct
      541 ccatcgccgc gaaattcttc ctcacgcacc tcgtgatcgc cgggatcgcg cttttgatcg
      601 ccggcgccgt aggcttcttc ctcgtgcgaa atctcgtgat ggcggacgcg gacgagaacc
      661 tgctcgcccg ggcgcgcatc gtcgccgaga cattccggcc gttgcttgcg gactccccgc
      721 agaacaggga gcgaatcgcc ggggaagggg accgcctcgg gaaggagatc ggcgcgcgca
      781 ttaccgtcgt cctgcccgac ggggccgtgg tcgccgattc caccgtgggg gcggccggtg
      841 tgccgggcat ggagaaccac gcgcaccacc ccgagatccg cgacgccctc tcggggacga
      901 cgggggtatc tctccgacga agcatcacgg tccgggagga acaacggtac gcggccctcc
      961 cgatcacgat cgggggcacg atcgtcgggg cggcccgcgc ctcggtcccc gtcgcgaccc
     1021 tcacccggcg cctgtggcag atcaccacga tcatctgggg aacgggattc gccgcgctcc
     1081 tcctcatcct ggggggaacc gccgtcatgg cgcggaggat cacgattcct ttggcggaga
     1141 tgcaggcggc ggcgaaggag atgggggccg gcaatcttgg gcgtcgcgtc cagatccgga
     1201 cgggcgacga actcgaagag atggcgaacg caatgaacag gatggcgtcg cacctcaccg
     1261 acacgatcgg gcagctggac gcgggcaaag cgcgcctgga gacgctgctc gcgaacctcg
     1321 acgacggggt catcgtcatc gccgcggacc ggtcggtccg gatgatgaac cgggacgccg
     1381 ggaagatctt cgacgcctcg gagacgatgg gcgcgggacg cccgtacccg gaagtgatcc
     1441 gccatcccga ggcgctcgcc ttcatcgacg ggtggatcaa gggagaggaa ccgaagccgc
     1501 gggacgtttc catcctgaca cgccatggaa gccgaatggt ccgatgttcc ggtacgacgg
     1561 tgcggtacct cggggaatcg aacgcggatg tcctcctcac ccttcgggac gtcaccgagg
     1621 agcggcggtt ggcgcaggtc aagagcgact tcgtgtcgaa tgcctctcac gagctgcgca
     1681 cgccgctgac gaacatccgt ggatacctcg aggcgatcca ggacgcggtc cgggaaggta
     1741 caccgcccgc cccgtccttc gtcgccgtgg cccacgcgaa cgccttgcgg atggagcgcc
     1801 tcatcgacga cctgctggag ctctcccggg ccgagtccac cggtgtccct ctggagtggg
     1861 aggaggtacc cctgcaggac tttctctccc gcgtcgccga gttccaccgc ctgcccgcgg
     1921 agcgggccgg gaaaacgctg gtggtggtcg cgggcgaggg aaccttgcgc gccgatgtcc
     1981 cgaagctggc cctcgccatg tcgaacctgg tggacaacgc catcaagtac gggaaagaag
     2041 gcgggcgagt caccgtgtcc gggaagatcg agccctcggg atgcactctg gaggtcaagg
     2101 acgacgggcc cggcatccca ccggaggaca ttccgcgcat cttcgagcgg ttctaccggg
     2161 tggacaaggg aaggtcgcgg gaactgggcg gcaccgggct cggcctgtcc atcgtgaaac
     2221 atatcgtcga gtcgcacgag gggatcctcc aggcggagag ccgcctcggg gtcggaacgc
     2281 gattcgtcat ccggatcccg gggacccccg ggtccctctc cacacccccg tgaggctgta
     2341 cctcgtccgc cacgcggagg cgatcgaacg gtccgggacg acaccggacg cctcccggta
     2401 cctgacgacg aaaggcc
//
LOCUS       DASVJL010000178         6607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_26743, whole genome shotgun sequence.
ACCESSION   DASVJL010000178 DASVJL010000000
VERSION     DASVJL010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26743"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VF357_03650"
     CDS             complement(<1..353)
                     /locus_tag="VF357_03650"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEX7523273.1"
                     /translation="MAEVKPFRGIHYDVKRVGDLTRVVAPPYDVISPEEQDALLRRHP
                     RNIVWIDFGKAKEGDGPGSDKYARAAALFSEWQAEGTLVRDPLPALYYYEQEFTIPGK
                     GTFVRKGFLGALKLSP"
     gene            complement(358..1104)
                     /locus_tag="VF357_03655"
     CDS             complement(358..1104)
                     /locus_tag="VF357_03655"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX7523274.1"
                     /translation="MTRLILLDAGRAGRSVTPNPRFPAEMISSLRAGGAVIFPTDTLY
                     GLGVDPCSETGLNRLLAAKRRDRGKPIPLLLSEVGQVDRWARHVPPAAVRLMDRFWPG
                     ALTLVLPADPGVHPAVTGGGDTVGLRIPDHPVPRALAALLSGAVTGTSANRSGNPGAW
                     GSAEEIVREFTGAVDWVLWGGAPVRASRCRGTPAVGRTGGKAPSGNARREGAGNSPGS
                     TVVRMIDDQPVLLREGVLPFCDIIEFLHRG"
     gene            complement(1187..1678)
                     /gene="purE"
                     /locus_tag="VF357_03660"
     CDS             complement(1187..1678)
                     /gene="purE"
                     /locus_tag="VF357_03660"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX7523275.1"
                     /translation="MMAGKVLILMGSVSDAETMRAAAEVLSGYGIPCEMTVASAHRSP
                     GRTQALARGAEKEGFSVIIAGAGAAAHLAGCVAAETVLPVIGVPLAGSGLGGFDALLS
                     TVQMPAGVPVATVAVGKAGAENAGHLAAQILSLSSPKLRAKIRAGRKAMADAVEEAAK
                     RLP"
     gene            complement(1675..2961)
                     /gene="purD"
                     /locus_tag="VF357_03665"
     CDS             complement(1675..2961)
                     /gene="purD"
                     /locus_tag="VF357_03665"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX7523276.1"
                     /translation="MGISVLIVGGGGREHALAWKIAKSPLVSKIYAAPGNPGIARHAE
                     CVPLATDDLDGLRNFAVSKRIDLTVVGPEAPLAAGLTDLLTKEGLLVCGPDRAGAQME
                     GSKVFMKTILRKYGIPTASFKVFDEYDEAEQYLLTHWLPVVVKADGLAAGKGVGVAQT
                     YEEGIAFLKDVMERRVFGAAGERVVIEECLSGEEASYIVFTDGDKCVPLPSSQDHKRI
                     GDNDTGPNTGGMGAYSPAPVVTPDVETRVHREIFEPLLAGLRAEGIVFRGILYAGLMI
                     ERGIPRVLEFNVRFGDPEAQPLFLRLKSDLVPLLLQCAQGKITDAKMEVDPRPAVCVV
                     MSSGGYPGKYEKGRPVGGIEEAEKEEGVVVFHAGTAMKEDRLVNHGGRVLGVTAIGDT
                     LMEAIARAYRAVDKIHWEGAYWRTDIGRKAFVRRNG"
     gene            complement(2986..4548)
                     /gene="purH"
                     /locus_tag="VF357_03670"
     CDS             complement(2986..4548)
                     /gene="purH"
                     /locus_tag="VF357_03670"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX7523277.1"
                     /translation="MARIHRAIVSVSDKTGVAQFCTALSRLGVELYASGGTAKLLREK
                     KVPVHLIEEYTGFPEMLDGRVKTLNPKIHGGLLALRGNPAHMKTIGEHGIVPFDMLVG
                     NLYPFEATIARPGCTREEAIENIDIGGPSMLRSAAKNSQSVAVVCDPADYPLVLAQLK
                     KNAGNLDEETMRELGRKAFALTARYDAAISNYLGAGPGGVEPFPVTFTAQWRKLQGLR
                     YGENPHQASSFYADTTLPDEPTLGGAEQLQGKELSYNNIVDIDAALQLALEFAIPAAV
                     IIKHTNPSGVGVSKRRLLDAFKKARECDPISAYGGVIGFNRPVNSETAREIANTFFEA
                     IIAPSYDKEARKILSAKKNLRVLATGGAFRWSGARTFEMKRVSGGLLLQTGDRHVLDP
                     KDLKVVTKRRPSPDELEAMLFAWKVCKHVKSNAIVYATKDRTVGVGAGQMSRVDSAKI
                     AVMKAQHPTKGTVVASDAFFPFRDGLDVAAEAGATAVIQPGGSVRDAETIAAADEHGM
                     AMVFTGVRHFRH"
     gene            complement(4606..6135)
                     /locus_tag="VF357_03675"
     CDS             complement(4606..6135)
                     /locus_tag="VF357_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX7523278.1"
                     /translation="MSREARVGIFVLLGLIVLTFFTFRVSKWGLIAEKGYRLTVDFDS
                     AAGLEPKSDVKMAGVRIGKVEEIQLVGNRARLVLRIQQGIRIPIDSVASIQTQGLLGE
                     KYVEILPGKDAQRNLPAGGQVANTLSPTNLDEMVRKLSAIGDDVKKFTESLSATFGTE
                     EGKKALGDILRDVRATTASLRTVVQGNEHAFQRIVSNIDRLTADLSDISVSNKQDVRA
                     TIANLRALSDTLKRETPSLVRKLEEMSDRVSGVVGDNRENLKESIQNLKTASARLDNT
                     LDAAGKVMAKIDRGEGTLGKLVNDNTAHTSLTATLDGINRYVRKTEQLKTFIDYHLEW
                     QQGPSEFKHYVNLRLQPTADKYYTIGVVDDPRGKFSSSTSTVTVNGVPTTYSEEKYDN
                     KLKVSALIARKFSGLTVKGGVMESTGGLGLDYELLKNRLTVGADAWDFTRKDLSSKAL
                     PPHLKLYGNYDIVKNFFVTGGVDDVLATEKNLRTLFFGFGIKFADEDIKTVLGAVPIK
                     P"
     gene            complement(6217..>6607)
                     /locus_tag="VF357_03680"
     CDS             complement(6217..>6607)
                     /locus_tag="VF357_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7523279.1"
                     /translation="IQRLMPAELSGGMKKRVGLARAIASEPDILLYDEPTTGLDPIMA
                     DVINDLIISLRETLGVTSITITHDMASAYKIADQIAMLYKGKIIEGGTPEQIRTTSNP
                     VVAQFVQGRAHGPITDESEEFVRFVSR"
BASE COUNT         1017 a         2334 c         2069 g         1187 t
ORIGIN      
        1 ggcgataatt tcagcgcccc gaggaaccct ttccgcacga aggtcccctt tccgggaatc
       61 gtgaactcct gctcatagta gtagagggcc ggaagcgggt cgcggaccag cgtcccctcg
      121 gcctgccatt cgctgaaaag agccgccgcc cgggcgtact tgtccgatcc cggaccgtcc
      181 ccctccttcg ccttcccgaa atcgatccac acgatgttgc ggggatggcg gcggaggaga
      241 gcatcctgct cctccggaga gatgacgtcg tacggcggcg ccaccacgcg ggtcaagtct
      301 cccacgcgct tcacgtcgta atggatcccc cggaacggtt tcacttcggc catggcgtca
      361 ccctctgtgc aggaattcga tgatgtcgca aaacgggaga accccctccc tcaacaggac
      421 cggttggtcg tcgatcatcc gcaccacggt ggacccgggg gagttcccgg cgccttcccg
      481 gcgtgcgttc ccggaaggcg ctttgccccc cgttcttcct actgcggggg taccccggca
      541 gcgcgaagcc cgtactgggg cgccccccca caagacccag tcgaccgccc cggtgaattc
      601 ccggacgatc tcctcggcgg acccccacgc accgggattc ccggaccggt tggccgatgt
      661 gcccgtgacg gcgccggaga gcagggccgc cagcgcacgc gggacggggt ggtcggggat
      721 acgcagtccc accgtgtctc ccccgccggt cacggccgga tggacacccg gatccgccgg
      781 gagcaccagc gtcagggcac cgggccagaa ccggtccatg aggcggacag cggcgggcgg
      841 aacatgccgc gcccaacggt cgacctgtcc cacctccgac aggagcagcg ggatcggttt
      901 cccccggtcc cgcctcttgg cggcgagcaa ccggttgagc cccgtttccg agcacgggtc
      961 cacgccaagc ccgtacaacg tgtcggtggg aaaaatcacc gcccctccgg cgcgcagcga
     1021 agagatcatc tccgcgggga agcgcggatt cggagtcacc gacctgccgg cacgaccggc
     1081 gtcgaggagg atcaggcgcg tcatgccggg cgcccccctc gcggagcgcc acgcctccgt
     1141 ccgtccgcga gggacgacca tggaaggcgt ccctcgggcg cgcaggtcat ggaagtcgct
     1201 tcgctgcctc ttcgacggcg tccgccatcg ccttccgtcc ggcccggatc ttcgcgcgaa
     1261 gcttcgggga cgaaagggaa aggatctgcg ctgcgaggtg ccccgcgttc tccgcccccg
     1321 cctttcccac ggcaacggtc gccaccggca cacccgcggg catctggacg gtcgagagca
     1381 gggcgtcaaa cccgccaaga cccgacccgg ccagcggcac cccgatcacc gggagcaccg
     1441 tctcggcggc cacgcacccg gcgaggtgcg ccgcggctcc cgccccggcg atgatcaccg
     1501 agaacccttc cttctccgcc ccacgggcca gcgcctgggt ccggcccggc gagcgatgcg
     1561 cggaggcgac ggtcatttca caagggatcc cgtagcccga caacacctcg gccgccgccc
     1621 gcatcgtctc cgcatcggag acgcttccca taaggatcag gaccttcccc gccatcatcc
     1681 gttcctccgc acgaacgcct tccgcccgat gtcggtgcgc cagtaggcgc cttcccagtg
     1741 gatcttgtcc acggcgcggt acgcgcgggc gatcgcctcc atcagcgtgt cgccgatcgc
     1801 ggtgacgccg agaacgcgcc ccccgtggtt caccaggcga tcctccttca tcgccgtgcc
     1861 ggcgtggaac accacgacgc cctcctcttt ctccgcctcc tcgattcccc cgacggggcg
     1921 ccccttctcg tacttccccg ggtagccgcc ggacgacatc acgacgcaga ccgccggcct
     1981 tggatcgacc tccatcttcg cgtccgtgat ctttccctgc gcacactgca atagcagggg
     2041 gacgagatcg cttttcaggc ggaggaagag aggctgcgcc tcggggtcgc cgaagcggac
     2101 gttgaactcg aggacccggg ggatcccccg ttcgatcatg agccccgcgt agaggatgcc
     2161 gcggaagacg atcccctcgg cacgaaggcc cgccagcagc ggctcgaaaa tctcccggtg
     2221 cacccgcgtc tcgacgtcgg gcgtcacgac gggtgcgggg gaatacgccc ccatcccgcc
     2281 ggtgttgggt ccggtatcgt tgtccccgat cctcttgtga tcctgcgagg aaggcagcgg
     2341 gacacactta tccccatcgg tgaagacgat gtacgacgcc tcctcccccg acaggcactc
     2401 ctcgatcacg acgcgctccc ccgcggcgcc gaacacccgt cgctccatca cgtccttcag
     2461 gaacgcgatc ccctcctcgt acgtctgtgc gacgccgacg cccttccccg ccgcgagccc
     2521 gtccgccttg accaccaccg gcagccagtg cgtcagcagg tactgctccg cctcgtcgta
     2581 ctcgtcgaaa accttgaagg aagccgtcgg gatgccgtac ttccggagga tcgtcttcat
     2641 gaagaccttg gacccctcca tctgcgctcc cgcgcggtcc gggccgcaga cgaggaggcc
     2701 ctccttcgtg agcaggtcgg tcagccccgc cgccagaggg gcctcggggc cgaccacggt
     2761 gaggtcgatc ctcttcgaca ccgcgaaatt ccgcaacccg tcgaggtcat ccgtcgccag
     2821 ggggacgcac tccgcgtgcc gcgcgatacc ggggttcccg ggcgcggcgt agatcttgga
     2881 cacgaggggg cttttcgcga tcttccaggc cagggcgtgc tcgcggccgc cgccaccgac
     2941 gatgaggacg gaaattccca ttccatcttc ctccgcttcg aagggctaat gccggaagtg
     3001 ccgcactccg gtgaacacca tcgccatccc gtgctcgtcg gccgcggcga tcgtttccgc
     3061 atcccggacc gacccgcccg gctggatcac agccgtggcc cccgcctcgg cggccacgtc
     3121 gaggccgtcg cggaacggga agaaggcgtc ggaggcgacc acggtcccct tcgtcggatg
     3181 ctgcgccttc atcaccgcga ttttcgccga gtcgacccgg ctcatctgcc ccgctcccac
     3241 gccgacggtg cggtccttcg tcgcgtagac gatcgcgttc gattttacgt gcttgcatac
     3301 cttccacgcg aacagcatcg cctcgagttc gtccggggag ggcctccgct tcgtcaccac
     3361 cttcaggtct ttcgggtcga gcacgtgccg gtcgcccgtc tgcaggagga gcccgccgct
     3421 gacccgcttc atctcgaacg tccgggcgcc cgaccaccgg aacgcgccgc ccgtggccag
     3481 gacgcggagg ttcttctttg ccgagaggat cttccgcgcc tccttgtcgt aggagggggc
     3541 gatgatcgcc tcgaagaagg tgttcgcgat ttcccgggcg gtctcggagt tcaccggtcg
     3601 gttgaagccg atcacgcctc cgtaggcgga gatggggtcg cactcgcgcg ccttcttgaa
     3661 ggcgtccagg aggcggcgct tcgagacccc cacgccggag ggattcgtgt gcttgatgat
     3721 gaccgcggcg gggatggcga attcgagggc gagctgcagc gccgcgtcga tgtcgacgat
     3781 gttgttgtag gagagctcct tcccctgcag ctgctccgcg cctcccagcg tcggctcgtc
     3841 gggcagcgtg gtgtctgcgt agaacgacga cgcttggtgc gggttctcgc cgtaccggag
     3901 cccctgaagc ttccgccact gggccgtgaa ggtcacgggg aacggctcca ccccccccgg
     3961 ccccgcgccg aggtagttcg agatcgccgc gtcgtaccgt gcggtcagcg cgaacgcctt
     4021 ccgtccgagc tcccgcatcg tctcctcgtc gaggtttccc gcgttcttct tcaactgcgc
     4081 gagaaccagg gggtagtccg cggggtcgca caccaccgcg accgactggg agtttttcgc
     4141 ggcggaccgc agcatcgaag gcccgccgat gtcgatgttt tcgatcgcct cctcccgcgt
     4201 gcagccgggg cgcgcgatcg tcgcctcgaa ggggtaaagg ttcccgacca gcatgtcgaa
     4261 cgggacgatc ccgtgctcgc cgatcgtctt catgtgcgcc ggattcccgc gcaaggcgag
     4321 gagcccgccg tggatcttcg ggttcaaggt tttcacccgc ccgtccagca tctcggggaa
     4381 cccggtgtac tcctcgatga ggtgcaccgg gaccttcttc tcccgcagca gtttcgccgt
     4441 gccccccgag gcgtacagct cgacgccgag ccgtgagagc gcggtgcaga actgcgccac
     4501 gccggtcttg tcggatacgc tgacgattgc ccgatggatt ctcgccatgg cctgggatcc
     4561 ttccttaggt atcgcgttgc ggtaattacg gatcggagtg cgtcgtcacg gcttgatggg
     4621 aacggccccc aggacggtct tgatgtcctc gtcggcgaac ttgatcccga aaccgaaaaa
     4681 cagggtccgc aggttcttct cggtcgccag cacgtcgtcc actccgccgg tcacgaaaaa
     4741 gtttttgacg atatcatagt tgccgtacag cttcaaatgg gggggcaggg ccttgctgga
     4801 gaggtccttg cgggtgaagt cccaggcgtc cgccccgacc gtcagccggt ttttcagaag
     4861 ttcgtagtcc agtcccaggc cgccggtgga ttccatcacg ccgcccttca ccgtgaggcc
     4921 ggagaatttc ctggcgatca gggcggagac cttcagcttg ttgtcgtact tctcttccga
     4981 atatgtcgtg ggcacgccgt tgacggtgac cgtcgatgtg ctggagctga acttcccgcg
     5041 cgggtcgtcc accactccga tcgtgtaata cttgtccgcc gtcggctgga gccggaggtt
     5101 cacgtagtgc ttgaactcgg acggcccctg ctgccactcc aggtggtagt cgatgaacgt
     5161 cttcaactgc tccgtcttcc ggacgtaccg gttgatcccg tcgagagtgg cggtgaggga
     5221 ggtgtgggcc gtgttgtcgt tgacgagctt cccgagggtc ccctcgccgc ggtcgatctt
     5281 ggccatcacc ttcccggcgg cgtccagcgt gttgtcgagg cgcgcggagg cggtcttgag
     5341 gttctggatg ctctccttca ggttctcgcg gttgtccccc acgacgccgc tcacccgatc
     5401 gctcatctcc tcgagcttgc gcaccagcga cggcgtctct ctcttcagcg tgtcggataa
     5461 agcccggagg ttggcgatcg tcgcgcggac gtcctgcttg ttgctcacgg agatgtcgga
     5521 aaggtccgcg gtgagccggt cgatgttcga cacgatccgc tggaaggcgt gttcgttccc
     5581 ctgaacgacg gtccgcaggg acgcggtggt ggcccggaca tcgcgaagga tgtccccgag
     5641 cgccttcttc ccctcctccg tcccgaaggt ggcggagagc gattcggtga acttcttgac
     5701 gtcgtccccg atcgccgaca gtttcctcac catctcgtcg agattcgtgg ggctcagggt
     5761 gttggcgacc tgcccccccg ccggcaagtt gcgttgcgcg tccttgcctg ggaggatctc
     5821 gacgtatttc tcgccgagga ggccctgcgt ctggatggag gccaccgagt cgatcgggat
     5881 acggatcccc tgttggattc gcaggaccag gcgggcgcgg tttccgacca gttggatctc
     5941 ctcgaccttc ccgatccgga ccccggccat tttcacgtcc gacttgggct ccagccccgc
     6001 cgccgaatcg aagtccacgg tgagccggta tcccttctcg gcgatcaacc cccacttgct
     6061 cacacggaac gtgaagaacg tgaggacgat cagccccaga agaacgaaga tcccgacccg
     6121 ggcctccctg gacatcgacg atgctccccc ctgaactgcg gcccgaccgg acctcgccgg
     6181 ccggactcct cgccccgaaa gatcggggat actatatcat cggctgacga agcggacgaa
     6241 ctcctcgctc tcgtcggtga tggggccgtg cgcgcgaccc tgcacgaact gcgcgaccac
     6301 cgggttcgac gtcgtccgga tctgctccgg cgtccccccc tcgatgatct tccccttgta
     6361 cagcatcgcg atctgatccg cgatcttgta ggcggaggcc atgtcgtggg tgatcgtgat
     6421 cgaggtcacc ccgagcgtct cccgcagcga gatgatcagg tcgttgatga cgtcggccat
     6481 aatcgggtcc agccccgtcg tcggctcgtc gtacagcagg atatcgggct cggaggcgat
     6541 cgcccgggcc agccccacgc gcttcttcat gccgccggac agctccgcgg gcatcagccg
     6601 ctggatg
//
LOCUS       DASVJL010000179         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1620812, whole genome shotgun sequence.
ACCESSION   DASVJL010000179 DASVJL010000000
VERSION     DASVJL010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620812"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..419
                     /gene="zapB"
                     /locus_tag="VF357_03685"
     CDS             186..419
                     /gene="zapB"
                     /locus_tag="VF357_03685"
                     /inference="COORDINATES: protein motif:HMM:NF017786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HEX7523280.1"
                     /translation="MPKMGEESFALIEKKITDLIQIVTALKKEKENLAGELVRKDGEV
                     RELTRKLAELSRERVDVKDRVDKILSRLDTIEL"
     gene            451..774
                     /locus_tag="VF357_03690"
     CDS             451..774
                     /locus_tag="VF357_03690"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX7523281.1"
                     /translation="MGNRIDVNIAGYALTVRTDRSEEHMARLAETLNGRVREIQKQGD
                     TANYLNVIMLAAMELADEVLTFEDRFREIKEQVEALRREREDLKTRVDRKSRDLLARL
                     DDALK"
     gene            794..974
                     /gene="ssrS"
                     /locus_tag="VF357_03695"
     ncRNA           794..974
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VF357_03695"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1184..>1618
                     /locus_tag="VF357_03700"
     CDS             1184..>1618
                     /locus_tag="VF357_03700"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX7523282.1"
                     /translation="MLVLERKEILRKEGRIRLRERAESPYSAEIGDRAQEHFLREFPP
                     LAGKTAALYCAMAGEVPTERIRHAYLAAGARLYYPRVTGRRTLAFYPHREGDGWKTGP
                     YGIPEPSNPAGGEPRLSGWDIIVVPGLAFDRRGNRLGHGFGYY"
BASE COUNT          334 a          458 c          564 g          262 t
ORIGIN      
        1 cgatccgtta catcggcgtc ggtgaaaagg ccggcgacct gcgttcgttc gacgcccggg
       61 acttcgccga ggcgctgttc tgacctcccc ccgcattcgc aattgaatcc gggcgatcga
      121 cggggtatcc ttcggaagga ccgccgaaat catcgtccgg cggggcggga tcacacatcg
      181 aggagatgcc gaagatgggc gaagagtcgt tcgcgctgat cgagaagaag atcaccgacc
      241 tgatccagat cgtgaccgcg ctgaaaaagg agaaggaaaa ccttgcggga gagctcgtgc
      301 gcaaggacgg ggaggtgagg gagttgaccc gaaaactggc cgaactttcg cgggagcgcg
      361 tcgacgtgaa ggaccgggtg gacaagatcc tctcccgact ggacaccatt gagttatagg
      421 ggcgaggcgg tataataaga aggaaagagg atggggaacc ggatcgacgt caacatcgcc
      481 gggtatgctt tgaccgtccg gaccgaccgg tcggaggagc acatggcgcg cctggccgag
      541 acgctgaacg gaagggtccg ggagatccag aaacagggcg acaccgcgaa ctacctgaac
      601 gtcatcatgc tcgcggcgat ggaacttgcc gacgaggtcc tgacgttcga ggaccggttc
      661 cgggaaataa aggagcaggt cgaggcgctg cggagggaaa gagaagacct gaagacccgg
      721 gtggaccgga aaagccggga cctcctggcg aggctcgacg acgcgttgaa ataggctggc
      781 aggaaattcg tcggggcccc tgccgtgtgc gtgatcgttg gcagagtttg agccaacacg
      841 tagagaatgg gatcccttcc gggcggtggt gagcatcccc ttacgccggg gaagcctgaa
      901 gcggcccaac cgggaaccca cctggtgaaa gccaggttca actcgaccgg cgacacggca
      961 ttcgcggggg cactttagac ggaacccggg cgagaaacgc ccgtttgcat agatcagggc
     1021 ggctataacc gttgttgcgg cgacgttctt atcgcggttc aatgcggcgg caatttttgt
     1081 tcgaataagg gtacccggcg tcacaggccg aaacgtttcc gcagcgcgag cgcagcaacc
     1141 ccgcggtttt cgcctcccgt ccacctccgt tgaaaggcgt ccgatgctcg tccttgagcg
     1201 caaggagatc cttcgcaagg aaggccgtat ccggcttcga gagcgggcgg agtccccgta
     1261 ctcggcggag atcggtgacc gtgcgcagga acactttctc cgggagtttc caccgctggc
     1321 ggggaagacc gcggccctgt actgcgcgat ggccggtgag gtccctaccg aacggatccg
     1381 gcacgcgtat cttgcggcgg gggcccggct ctactacccc cgtgtcacgg ggaggcgaac
     1441 gctcgccttc tacccgcacc gggaagggga cggatggaaa acggggccgt acgggatccc
     1501 cgagccgtcg aacccggcgg gcggcgagcc gcggctgtcg gggtgggaca tcatcgtggt
     1561 tccgggcctc gccttcgacc ggcgggggaa tcgtcttgga cacgggttcg ggtattac
//
LOCUS       DASVJL010000180         3454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1339846, whole genome shotgun sequence.
ACCESSION   DASVJL010000180 DASVJL010000000
VERSION     DASVJL010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339846"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VF357_03705"
     CDS             <1..359
                     /locus_tag="VF357_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7523283.1"
                     /translation="GQRLIEEGNVRLAGEIVRPSTRLKGGEPMTVVIPPPVPPHLLRE
                     MELRGGVEVTFVETSSPAWDGGVREGDVLLSINRETVRGLDEYRKALSRLPQGRPAFA
                     LVYRDGVQLYVALKSR"
     gene            370..636
                     /locus_tag="VF357_03710"
     CDS             370..636
                     /locus_tag="VF357_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523284.1"
                     /translation="MGMVKDTDVARRIARAVVSDIALYNAKKVEEGVRNDTLFDLLKE
                     EIEEGKGYYQSRVDPEIAKSTNYFDQALVDVLLKPAGHIPSKIW"
     gene            638..1603
                     /locus_tag="VF357_03715"
     CDS             638..1603
                     /locus_tag="VF357_03715"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7523285.1"
                     /translation="MPNGPRGTHRLTVPADRAGERLDRYLPEAIPGISRARGQRLIEE
                     GNVRLAGEIVRPSTRLKGGEPMTVVVPPPVPLDMMAEDLPLHVLYEDSHLLVVDKPAG
                     MVVHPAPGHPRGTLVNALLGRSDTLSGIGGVSRPGIVHRLDRDTSGILVVARTDAAHG
                     TLSARFAAHAMDRKYRGIVFGGPPADRGTVSTRIGRHPFHRKKMAVLSAGGREAVTRY
                     RRLETFGPFSLLEFGLETGRTHQVRVHCAHLGCPIVGDDVYGRSRKIPLGKGTSAGTV
                     TVSRFLLHAFHLAFPHPVTGEPLSFTIPDPPEFAAFRDAVTEAYG"
     gene            1637..2365
                     /locus_tag="VF357_03720"
     CDS             1637..2365
                     /locus_tag="VF357_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEX7523286.1"
                     /translation="MNPLPHFEQSPLLRSVPGVYHAFLGVDPVEGRGRRERLREVFSI
                     AAETVGTLKQVHSASVLSFEPGDGDVPGGWKREGDALWTVSPGTGVGVHTADCVPILL
                     AHRKIRVAAAVHAGWRGLAAGVVEETVHVLAGRFGNAAVGGFVAVAGPCARGCCYEVG
                     EETAEALHGLPGEASLLRKGKGPGKWTADLQGIALAALRGVGIPAGQSEAAGPCTICS
                     PRFHSFRREKSTTSRQLSFLYIRD"
     gene            2532..>3454
                     /gene="rho"
                     /locus_tag="VF357_03725"
     CDS             2532..>3454
                     /gene="rho"
                     /locus_tag="VF357_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX7523287.1"
                     /translation="MNLKELKGMRIGDLTEIARKMNVDGAAGLKKQELIFAILQSQTD
                     QEVIVSGEGVLEVLPDGYGFLRAPDSNYLPGPDDIYVSPSQIRRFGLRTGDTVSGQIR
                     APKGDERYFALLKVEKVNYEDPEISKDKILFDNLTPLYPQEKFTMEYAHDNYATRIID
                     LIAPIGKGQRALITSPPRAGKTVILQNIANGLTKNHPEAVLIVLLIDERPEEVTDMQR
                     SVVGEVISSTFDEPAQRHVQVAEMVLEKAKRLVEHKKDVVILLDSITRLARAYNAVVP
                     PSGKVLSGGVDANALQKPKRFFGAARNIEEGG"
BASE COUNT          599 a         1114 c         1181 g          560 t
ORIGIN      
        1 gcggtcagcg gttgatcgag gaggggaacg tccggctggc gggagagatc gttcgtccgt
       61 cgacacgcct gaagggcggg gagccgatga ccgtcgtcat ccctccgccc gttccgcctc
      121 acctgctgcg ggagatggag cttcgcggag gcgtcgaagt gacgtttgtc gagacctcca
      181 gcccggcgtg ggacggcggc gtccgggaag gggatgtcct tctctccatc aaccgggaaa
      241 cggttcgcgg cctcgacgaa taccggaagg ccttgtcgcg actcccccag gggcggccgg
      301 ccttcgccct ggtctaccgg gacggtgtcc agctttatgt ggcgctgaaa agcaggtaag
      361 gaggagccga tgggaatggt gaaggacacg gacgtggccc ggaggatcgc ccgggcggtg
      421 gtctcggaca tcgcgctgta caacgcgaag aaggtcgagg agggagtccg gaacgacacc
      481 ctgttcgatc tcctgaagga ggagatcgaa gagggaaagg gatactacca gagccgtgtc
      541 gaccccgaga tcgcgaagag cacgaattat ttcgaccagg ccctggtgga tgtcctgctc
      601 aagccggccg gccacatccc ctcgaagatc tggtagcgtg ccgaacggtc cgcgcgggac
      661 gcaccggctg accgttccgg ccgaccgggc gggggaacgc ctcgaccggt atcttccgga
      721 ggcgatcccc gggatctccc gggcgcgagg gcagcggctg atcgaggagg ggaacgtccg
      781 gctggcggga gagatcgtgc gtccgtcgac ccgtctgaag ggcggggaac cgatgaccgt
      841 cgtcgtccct ccgcccgttc cgctcgacat gatggcggag gatcttcccc tccacgtgtt
      901 gtatgaagat tcccacctgc tggtcgtcga taaacctgcc ggcatggtgg ttcaccccgc
      961 gccggggcac ccacgcggaa cgctcgtgaa cgcgctcctt gggcgctccg acaccttgtc
     1021 cgggatcggg ggcgtttccc gcccggggat cgtccaccga ctcgatcggg acacctccgg
     1081 aattctcgtg gtcgcgagga ccgacgccgc ccacggaacg ctgtccgccc ggtttgccgc
     1141 ccacgcgatg gaccggaagt accgtgggat cgtcttcgga ggcccgcctg cggacagggg
     1201 gaccgtgtcg acgcggatcg ggaggcaccc cttccaccgg aaaaagatgg cggtgctgtc
     1261 ggcgggggga cgggaggccg tcacccggta ccggaggctc gaaacgttcg gccccttctc
     1321 ccttctcgag ttcgggctgg agacgggacg cacccaccag gtgcgggtgc actgcgcaca
     1381 tctcggctgt ccgatcgtcg gggacgacgt gtatggaagg tcgcggaaga tccccctcgg
     1441 gaaggggacg tccgcgggga cggtgaccgt ctcccgcttt ctcctgcacg ccttccacct
     1501 ggcgttcccg cacccggtta ccggggagcc tctttccttc accatccccg acccgccgga
     1561 gttcgccgct tttcgcgacg cggtcacaga agcctatggg tgaaccggga ggaaggaaag
     1621 gagtgtcccc cgcccattga acccccttcc acacttcgag cagtctccac tgctgcgttc
     1681 cgtgccgggg gtctaccacg ccttcctcgg cgtggatccg gtggagggac gggggcgccg
     1741 cgaacggctc cgggaggtct tctccattgc cgcggaaacg gtcgggacgc tgaagcaggt
     1801 ccactcggcg tccgttcttt ccttcgaacc aggagacggg gatgtccccg gcgggtggaa
     1861 gcgggaggga gacgccctct ggacggtgag ccccgggacc ggggtggggg tccacacggc
     1921 ggattgcgtt ccgatccttc tcgcccaccg gaagatccgc gtagccgcgg ccgtccacgc
     1981 ggggtggcgg gggctcgccg ccggggtcgt cgaggaaacg gtccacgtcc ttgcggggcg
     2041 attcgggaac gccgccgtgg gaggattcgt cgccgtcgcg ggcccctgcg cgcggggatg
     2101 ctgctacgag gtcggggagg aaacggcgga ggcactccac ggtctgccgg gggaggcttc
     2161 gctcctgagg aaggggaagg ggccggggaa gtggaccgcc gacctccagg ggatcgccct
     2221 cgcggcgctc cggggcgtcg ggattcccgc cggacaatcg gaggcggcgg gaccctgtac
     2281 gatctgctcc ccccgcttcc actccttccg ccgcgaaaaa tcgacgacct cccggcagtt
     2341 gagttttctc tacatcaggg attgacgggg gggtttttct aaggtagtct tatggcttcc
     2401 ggaccagatc agcgcacatt cggcgataat tcactgccct ttggagtgcc ttccgagcgt
     2461 ttttccacca cgttgaagac gttggttgcg atttgccgca ggagaatacc catgttcccg
     2521 ggagagcgtg catgaacctg aaagagctga aaggaatgcg gatcggcgat ctgaccgaga
     2581 tcgccaggaa gatgaacgtg gacggggccg ccggcctgaa aaagcaggaa ctcatcttcg
     2641 ccatcctcca gtcccagacc gaccaggagg tgatcgtctc cggagagggg gtcctcgagg
     2701 ttctccccga cggctacggg ttcctccgcg cccccgactc caactatctc ccgggccccg
     2761 acgacatcta cgtctccccc tcgcagatcc gccgcttcgg cctgcgcacg ggggacaccg
     2821 tcagtggaca gatccgtgcg cccaaggggg acgagcggta cttcgccctc ctgaaggtcg
     2881 agaaggtcaa ctacgaagat cccgaaatca gcaaggacaa gatcctcttc gacaacctga
     2941 cgcccctcta cccgcaggaa aagttcacga tggagtacgc gcacgacaac tacgccaccc
     3001 ggatcatcga cctcatcgcg ccgatcggca aggggcagcg cgcgctgatc acctccccgc
     3061 cgcgggcggg gaagacggtc atcctccaga acatcgcgaa cggcctcacc aagaaccacc
     3121 ccgaggccgt cctcatcgtg ctgctcatcg acgagcggcc cgaggaggtg accgacatgc
     3181 agcgcagcgt cgtgggcgag gtcatctcct ccacgttcga cgagccggcg cagcgtcacg
     3241 tccaggtagc ggagatggtc ctggagaagg cgaagcgctt ggtggagcac aagaaggacg
     3301 tggtcatcct gctcgacagc atcacgcggc tggcgcgcgc ctacaacgcc gtcgtccccc
     3361 cgtcggggaa ggtcctctcc ggaggcgtcg acgccaacgc cctccagaag ccgaagcggt
     3421 tcttcggggc ggcccggaac atcgaggagg gggg
//
LOCUS       DASVJL010000181         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1621129, whole genome shotgun sequence.
ACCESSION   DASVJL010000181 DASVJL010000000
VERSION     DASVJL010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621129"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VF357_03730"
     CDS             complement(<1..207)
                     /locus_tag="VF357_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX7523288.1"
                     /translation="MSHGNFILREPEYEALLWALRKLLVDASAKVVFLVDKDGTLLAS
                     AGDAVGFDTTSLASLAAGNIAATGG"
     gene            complement(252..1541)
                     /locus_tag="VF357_03735"
     CDS             complement(252..1541)
                     /locus_tag="VF357_03735"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7523289.1"
                     /translation="MDPRVPLLLFGSFTVLLFLNFPIAAALGISSVLSVLAFDLAPLQ
                     LIPQQLFSATDSWTLLAVPFFILAGNLLAETAISERLIRLAMVIVGQFPAGLAMVAVI
                     VSIFFAGISGSGPADTAALGAILIPGMVKAGYDRRSASALIAAGGGIGIIIPPSIALL
                     IYGVVANASVSKLFIAGILPGMLVGLSLFVVSYLLFGKDPNIRPEKRGSLREIGSAFK
                     DAFWGLMAPLIILGGIYGGIFTATEAATVAVVYGVIVGFFIHRDLKLADLPEILVDTG
                     VTTGVVMLLVCTASVFAWVLNTSGVAAAAAAGLLHLATGKLVGLLLINAILLVAGCFM
                     DAISIFYVFLPILLPVVNALGIDLVHFGVVMTVNMAIGQITPPVGVNLFVACRIARIS
                     MKEISRAVLPMVVAQAVALLVISFWPDLSLFLTRFMR"
     gene            complement(1545..>1872)
                     /locus_tag="VF357_03740"
     CDS             complement(1545..>1872)
                     /locus_tag="VF357_03740"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7523290.1"
                     /translation="LGSSAAVKRKAHLGLSMVYDAFPANFRPAVDVLILLGTGAFFSA
                     TAWYGWKVVVLQMENRMMASLGYPSWIVGLAVPVGSVLFVVRGVETWWRHRQGAPETA
                     PGTPGI"
BASE COUNT          406 a          606 c          634 g          226 t
ORIGIN      
        1 acccccggtg gcggcgatgt ttcccgcggc gagggaggcc agggacgtcg tgtcgaaccc
       61 gacggcatcc ccggcggaag cgaggagggt cccgtccttg tcgaccagga agacgacctt
      121 cgcggaggcg tccaccagca atttccgaag ggcccaaaga agcgcctcgt actccggctc
      181 ccggaggata aaattcccgt gactcatagg cgaaaagata ccacacacat ccccaaagaa
      241 aaagctgccc cctaccgcat gaaccgggtg aggaagaggg agaggtccgg ccagaaactg
      301 atgacgagaa gcgccacggc ctgggcgacg accatcggga ggacggcccg ggagatctcc
      361 ttcatggaaa tgcgggcgat cctgcacgcg acgaagaggt tgaccccgac cgggggcgtg
      421 atctggccga tcgccatgtt caccgtcatc acgaccccga aatggaccag gtcgatcccc
      481 agtgcgttca cgaccggcag aaggatcggc aggaagacgt agaagatcga gatcgcgtcc
      541 atgaagcagc ccgccaccag cagaatcgcg ttgatcagca gcagccccac cagcttcccg
      601 gtcgccagat gcagcagccc cgcggcggcc gcggcggcga cgcccgaggt gttgagcacc
      661 caggcgaaga ccgaggcggt gcacacaaga agcatcacca ccccggtggt gacccccgta
      721 tccacgagga tctccggaag atcggcgagc ttcaggtccc ggtggatgaa gaagccgacg
      781 attacgccgt agaccaccgc cacggtggcg gcctccgtgg cggtgaagat gccgccgtag
      841 atccctccga ggatgatgag gggggccatg agcccccaga aagcatcctt gaacgcggat
      901 ccgatctccc gaagggaccc ccgcttttcc ggccggatgt tcgggtcctt cccgaagagc
      961 aggtacgaga cgacaaacag cgacaagccg acgagcatgc cggggaggat tccggcgatg
     1021 aacagcttcg acacggaggc gttggccacc accccataga tcagcagggc gatggaaggg
     1081 gggatgatga tcccgatgcc tcccccggcc gctatgaggg ccgacgcgga gcgccggtcg
     1141 tacccggcct tcaccatgcc cgggatgagg atggccccca gcgccgccgt gtcggcggga
     1201 ccggagccgg agatgccggc gaagaagatg gagacgatca ccgcgaccat ggccagcccg
     1261 gccgggaact gccccacgat gaccatggcc agccggatca ggcgctccga gatcgccgtt
     1321 tccgcgagga ggttccccgc gaggatgaag aacggcaccg cgaggagggt ccacgagtcg
     1381 gtcgcgctga agagctgctg ggggatgagc tgcagcgggg ccaggtcaaa ggccagcacg
     1441 gagaggacgc tcgagatgcc gagcgcggcg gcgatcggga agttcaggaa cagcaggacg
     1501 gtaaaactcc cgaacaggag cagcggaaca cgcgggtcca tcggtcagat gccgggcgtg
     1561 ccgggcgccg tctccggcgc gccctgccgg tgacgccacc acgtctcgac gccccggacg
     1621 acgaagagaa ccgacccgac gggcacggcc agcccgacga tccagctcgg gtaccccaac
     1681 gacgccatca tccggttctc catctggagg acgaccacct tccacccgta ccaggcggtc
     1741 gcggagaaga aggcgccggt cccgaggagg atcaggacgt ccaccgccgg acggaagttc
     1801 gcggggaacg catcgtagac catggagagc ccgaggtggg ccttccgttt caccgccgcc
     1861 gaggagccga ga
//
LOCUS       DASVJL010000182         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1247180, whole genome shotgun sequence.
ACCESSION   DASVJL010000182 DASVJL010000000
VERSION     DASVJL010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247180"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1061
                     /locus_tag="VF357_03745"
     CDS             90..1061
                     /locus_tag="VF357_03745"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7523291.1"
                     /translation="MPLNVFVTGAAGFIGSHVAEALLARGDRVYGLDNFDPFYDRSVK
                     EANLSALFGRPGFSFLEGDIRDASALARWGEGIRPDALIHLAAKAGVRPSVADPVGYA
                     DVNIHGTIRMLEWARDRKVPKVLFASSSSVYGGNTKVPFCEDDFVDHPVSPYAATKKA
                     GELLCHTYCHLYGMNVAALRFFTVYGPRQRPEMAIHKFTRRILEGKEIDLYGDGSSRR
                     DYTYIEDIVSGVLGALTAPPGYRVYNLGESATISLSDLVALIEKACGKAAVRRYKTPQ
                     PGDVPVTYADISRARKEIGYDPRTPIERGVTLFVDWYRRRADTEARR"
     gene            1079..>1571
                     /locus_tag="VF357_03750"
     CDS             1079..>1571
                     /locus_tag="VF357_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523292.1"
                     /translation="MGDGIRFRFDFSLVGESNLRGGDGIAAPDFRKMMALVDDAVEKL
                     AVKHRRGEIGFPDLPFLEKEARAISRDAAALRAKCTHLLVLGIGGSALGTKAVHEAVG
                     GAGRPGMALSVGDNVDPDAFFPLLAKLPMKKTVVVAISKSGGTAETNAQLALAIAALK
                     KAVG"
BASE COUNT          270 a          515 c          527 g          259 t
ORIGIN      
        1 cgcttcaagg actccttttt ggaactttgc tattctggga taatattgga ggagaaacgg
       61 cggaatgttc caatccggga ggttcgagcg tgcccctgaa cgtgttcgtc accggcgctg
      121 ccggcttcat cggatcgcac gtagcggaag cgctcctcgc ccgcggagac cgggtctacg
      181 gcctcgataa cttcgacccg ttctacgacc ggtccgtcaa ggaggcgaac ctctccgccc
      241 ttttcgggcg cccgggcttc tcctttctcg agggggacat ccgcgacgcc tccgccctcg
      301 cccgctgggg ggaggggatc cgtcccgacg ccctgatcca ccttgccgcg aaggccgggg
      361 tgcgaccgtc ggtcgccgat ccggtcggct acgcggacgt gaacatccac ggcacgatcc
      421 ggatgctcga gtgggcccgg gatcgaaagg tcccgaaggt cctcttcgcc tcctcctcgt
      481 ccgtctacgg aggcaacacg aaggttccct tctgcgagga cgacttcgtc gatcacccgg
      541 tgagccccta cgcggcgacg aagaaggcgg gggagcttct ctgccacacg tattgccacc
      601 tgtacgggat gaacgtcgcg gccctgcgct tcttcaccgt gtacggcccc cgccagcgcc
      661 ccgagatggc gatccacaag ttcacccgga ggatcctgga ggggaaggag atcgacctgt
      721 acggcgacgg ctcctcccgt agggattaca cctatatcga ggacatcgtc tccggggttc
      781 tcggcgcgct gaccgccccc ccggggtacc gggtctacaa cctcggggag tcggccacga
      841 tctccctttc ggacctggtc gcgctgatcg aaaaggcgtg cgggaaggcc gccgtgcggc
      901 gctacaagac gccgcagccc ggggacgtcc ccgtgacgta cgcggacatc tcccgcgcga
      961 ggaaagagat cggctacgac ccgcgcacgc cgatcgagcg cggcgttacg cttttcgtcg
     1021 actggtatcg ccggcgggcg gacacggaag cacgcaggta agaaggaggg agggcggcat
     1081 gggcgacggg atccggttcc gcttcgattt ctccctcgtg ggagagagca acctccgcgg
     1141 aggggacggg atcgccgcgc ccgacttccg gaagatgatg gcgctggtcg acgatgcagt
     1201 ggaaaaactc gcggtgaaac atcgacgcgg ggagatcggc ttccccgacc tgccgttcct
     1261 cgagaaggag gcccgggcga tctcccggga cgcggcagcc cttcgcgcga aatgcaccca
     1321 cctcctcgtc ctcggcatcg gcggttcggc gctgggaacg aaagcggtgc acgaggcggt
     1381 cggaggcgcg ggacgccccg ggatggcgct atccgtgggg gacaacgtcg accccgacgc
     1441 tttcttcccc ctcctggcga agctcccgat gaagaagacc gttgtggtgg ccatcagcaa
     1501 atcggggggg acggcggaga cgaacgccca gctcgcgctc gcgatcgccg cgttgaagaa
     1561 agccgtcggg a
//
LOCUS       DASVJL010000183         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_965756, whole genome shotgun sequence.
ACCESSION   DASVJL010000183 DASVJL010000000
VERSION     DASVJL010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965756"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VF357_03755"
     CDS             complement(<1..242)
                     /locus_tag="VF357_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7523293.1"
                     /translation="MSFGTLYVVATPLGNLEDITFRAVRILKEAPVIACEDTRRTVKL
                     LNRYEIRTPMVVFHEYNKARAGAGILRRLREGESVAL"
     gene            complement(252..1553)
                     /locus_tag="VF357_03760"
     CDS             complement(252..1553)
                     /locus_tag="VF357_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX7523294.1"
                     /translation="MYWEPDKECMDREELEQLQLERLQSTLNRVYAHVPFYRKKFDAL
                     GIAPEEIGSLSDLSRLPFTTKEDVRGNYPYGLFAVPLREVVRIHASSGSSETSSVVGY
                     TRNDIMMWSKLVARILVAGGVTKDDVVQVSFRYGLFTGGFGLHYGAERLGASVIPASS
                     GNTSRQIQIMKDFKTTALVSTPSYAMLVADTIREMGISVSALSLKYGLFGGEPWSEKM
                     RQEIQDALGIVATDNYGLSEVIGPGVSGECLERNGLHINEDHFLVEVIDPKTYQPVPP
                     GQTGELVITTLTKEAFPMIRYRTRDLTSLQKGDCPCGRTGRRMSRLAGRTDEMLIIRG
                     MKVSPAKIESLLFEIEGNEPNYRIMIDRKGAMDEVTVLVEAAGEAPFGQERRHVGTTV
                     ETIRKRLTHELGVTVDVKLVEKKALEPVDGKMMRVIDLRKL"
     gene            complement(1570..>1772)
                     /locus_tag="VF357_03765"
     CDS             complement(1570..>1772)
                     /locus_tag="VF357_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523295.1"
                     /translation="VTRLRGEGSTVLLVEQNAKAALGIADRGYVLETGRIIMEGTAED
                     LLLNREVQRAYLGRDLDAEGTM"
BASE COUNT          278 a          632 c          523 g          339 t
ORIGIN      
        1 agcgccacgc tctccccttc gcggaggcgc cgcaggatcc ccgcccccgc ccgcgccttg
       61 ttgtattcgt ggaagacgac catcggcgtg cggatctcgt agcggttcag cagtttcacc
      121 gttcggcgcg tgtcctcgca ggcgatcacc ggcgcctcct tgaggatgcg cacggcgcgg
      181 aaggtgatgt cctccaggtt ccctagcggt gtcgccacga cgtacagcgt cccgaaactc
      241 acatcgaccc cctacagttt tctcagatca atgacgcgca tcatcttccc gtcgaccggc
      301 tccagggctt tcttctcgac gagcttcacg tccaccgtca cccccagttc gtgggtcagc
      361 cgcttccgga tcgtctccac cgtcgtcccg acgtgccggc gttcctgccc gaacggcgcc
      421 tccccggcgg cctcgacgag gaccgtgacc tcgtccatcg cccccttccg gtcgatcatg
      481 atccggtagt tcggctcatt cccctcgatc tcgaagagca gggactcgat cttggcgggg
      541 gacaccttca tcccgcggat gatcagcatc tcgtccgtcc tgcccgccag gcggctcatc
      601 cggcgccccg tccgcccgca cgggcaatcc cccttctgga ggctggtgag atcccgggtc
      661 cggtaccgga tcatcgggaa cgcctccttg gtcaacgtcg tgatgacgag ctcccctgtc
      721 tggccgggcg gcacgggttg ataggttttc gggtcgatga cctcgacgag gaagtggtcc
      781 tcgttgatgt gcagtccgtt gcgctcgagg cactcgccgg agacccccgg cccgatgact
      841 tccgagagcc cgtaattgtc cgtggcgacg atcccgaggg cgtcctggat ctcctggcgc
      901 atcttctcgg accatggctc ccctccgaag aggccgtact tgagggagag cgccgaaacg
      961 gagatcccca tctcgcggat cgtgtcggcg accagcatcg cgtaggaagg ggtggacacc
     1021 agcgccgtcg ttttgaagtc cttcatgatc tggatctggc gggaggtgtt tccgctggac
     1081 gccgggatca ccgaggcgcc gagccgctcc gcgccgtagt gcagcccgaa cccccccgtg
     1141 aacaacccgt accggaacga gacctgaacg acgtcgtcct tggtcacccc cccggcgacg
     1201 aggatgcggg ccaccagctt gctccacatc atgatgtcgt tgcgcgtgta gccgacgacc
     1261 gacgacgtct ccgacgagcc ggacgatgcg tggatgcgga cgacctcccg aagcgggacc
     1321 gcgaacagcc cgtaggggta attccccctc acgtcctcct tcgtcgtgaa cggcagccgg
     1381 gagaggtcgg agagcgaccc gatctcctcc ggggcgatcc cgagcgcatc gaacttcttc
     1441 cggtagaacg ggacgtgcgc gtacacgcgg ttgagcgtcg actgcagccg ctcgagctgg
     1501 agctgctcga gctcctcccg atccatgcac tccttgtccg gttcccagta cattccgtcc
     1561 tccgatccgt tacatcgttc cttccgcatc cagatcccgg ccaaggtagg cccgttgcac
     1621 ctcgcggttc aagagcaggt cttccgccgt cccttccatg ataatcctgc cggtttcaag
     1681 aacatacccg cggtccgcga tgcccagcgc ggccttcgcg ttctgttcga cgagcagaac
     1741 cgtgctgccc tccccccgga gcctcgtgac ga
//
LOCUS       DASVJL010000184         9450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1340477, whole genome shotgun sequence.
ACCESSION   DASVJL010000184 DASVJL010000000
VERSION     DASVJL010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9450
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340477"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3756)
                     /locus_tag="VF357_03770"
     CDS             complement(<1..3756)
                     /locus_tag="VF357_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7523296.1"
                     /translation="MSDPFTRQEDSWPLGLPPSLGNPLPQRMLALTIHRDEYGPPSRS
                     VRMESLPAPRLKPNEAKRVLVAILATGPNFNTNFAALGLPVPVFGKGDTASVHVPGSD
                     ALGIVVDAGPGVSAVKVGQAVILDSWTGRNIRGYETHDGFNAQFAVVDEERAVPVTGA
                     LRNLTPERLAAMLLTYGTAYRAVAERLSVRPGDAVLVMGGGKGTSFPGAQIAKSLGAR
                     VLLVGSNHELARDLISRGMADAFVDRRSVPKEAFGVIPAGMPYAEWEQRTDPFRRAVF
                     EANGGKPVDKIFEHTGGENFPLLISVLSPGGSLAFFGATGKGLKGEYKETFFHDGRRF
                     VMDARWVWMRQKQVLFRRAKPAAIFDELGLPPGRRVLVWGADRSAREFVAAARARTAE
                     VVVIASATREKRGIAKLSRMGVPPTHILDRDGFSLPEDMPDPLTPEGGLNPEYASGFT
                     RPAQALGKALWKVFGPRVSPDVVVDRVDQDTLHFSTFVARDFDEKDVFPCGAVVLRGK
                     SDLSIRGSHMYRASQAADVIRLLSAGKIAMDQEDLEITDLPGLPRLQQMMLDGTMRKP
                     KGVALVQANRQGRTVADYEEAFRGEIVQAADPAARKFVDVRMAGDVAVVTLTRPDSLN
                     ALSEALLSQFAAVVREAGILGTIGGRTVEAIVLNGAGRSFVAGADVREFHGKTAEVVD
                     ALAWKNISVFTELENLALPVVALVDGFALGGGNELAMSAHYRIVTENALLGQPEVKLG
                     IIPGYGGMQRLPRLVGPSKAADICINGEPVDGSTAVSLGWADEFVPASAALPRAVAAA
                     RDFAAGKRPVPRRDWDGLAGGWMEELARLFARPEVKAILEAPTPGKESAGNLRAARLA
                     AARDALLAMKHGFEHGFEEGLRNDARMFGAVAASPSGQEWVGRFLEKDAAQSSFLTIL
                     PPAEPDGKKPPPITKKAPGDRPITFVEIGEIAAILRKKMKSGHVTPLPKKILNEVISL
                     LKDRPSPAILAEIAPEISGNAGARIASLLSRGKISVRVVDLGPDEGFRRENARPACEV
                     LLVEKDGEILWEGKKRFRTEPGTFPRVFLPKAFLEQNASRPHVVYQAIVHPILEWVFG
                     YPHMIAVLCESAYNATPPEGAGSLSDLNRYIAEGAGRDSDFPYFDRILVDAYEPEDFR
                     KEELSNFFGGDEAKVGTVLARAREMGVRYRELVAEIREGVLGEIAARQVSAGRAALDE
                     GDAERALLLLRNLVLSPETPSSYRKEAANGIAVAIRSYALGADPAFEG"
     gene            4175..4375
                     /locus_tag="VF357_03775"
     CDS             4175..4375
                     /locus_tag="VF357_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7523297.1"
                     /translation="MAFGTVKWFNDAKGFGFITEEGGEDIFVHFSEIKGEGFRTLAEG
                     QRVEFEVTSGPKGKKAANVRKA"
     gene            4445..5554
                     /gene="ald"
                     /locus_tag="VF357_03780"
     CDS             4445..5554
                     /gene="ald"
                     /locus_tag="VF357_03780"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX7523298.1"
                     /translation="MRIGVPRETKPGENRVAITPLAVRALRDFGTEVVVETGAGTVSG
                     FEDREYLESGAAIVSKASEAWSADLVVKVKEPLAPEFPFLSESSALFAFLHLAALPGL
                     TGELLARRVTAVAYETVTSAGALPILRPMSEVAGILSIQVGARGLEKGCGGRGILLSG
                     AEGSPPGDVIVLGAGVAGRNAARIAARIGAIVTILDVNPEKLALAKRECGGNVRTIRS
                     TAEAVNDAVREADLVVSTVLVAGERAPLLVPRAMVRGMRTGAVVVDIAIDQGGSIETS
                     RPTTHEAPYFIDERVVHYCVTNMPAAVPRTSTRGLVAATLPYLLSFARQGVAGALRLD
                     EGLLAGLNTCGGHVTCEGVAKAFGKPFLPPRDAIR"
     gene            complement(5487..6152)
                     /gene="coaE"
                     /locus_tag="VF357_03785"
     CDS             complement(5487..6152)
                     /gene="coaE"
                     /locus_tag="VF357_03785"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX7523299.1"
                     /translation="MRVFGLTGNIGSGKSTVAAMLREAGIPVLDADRISKEVTAPGGR
                     AYDAVVRAFGKAIVRDDGTIDRKRLGEIVFSDPASRERLERITHPAILEAMKEAISGI
                     EREGHRAAVVEATLIHESGKTGLFEAVISVACDRETAISRLAARDGMSRSQAEARLRA
                     QRDADRKAGASDYVIDNSASPEETSRQVLALARVLLENGVTGSHPSGGGTASRTPSPP
                     PRR"
     gene            6255..6575
                     /locus_tag="VF357_03790"
     CDS             6255..6575
                     /locus_tag="VF357_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX7523300.1"
                     /translation="MAETTVKGFFEGLSDKLNAKPEKVAGMNCVYQFRVTEAAWNVKL
                     ADGKVAVAEGEVPSPNCTITMAEADFLDLVSGKLNGQMAFLTGKLKVAGDMGLALKLG
                     SFIG"
     gene            6587..7372
                     /locus_tag="VF357_03795"
     CDS             6587..7372
                     /locus_tag="VF357_03795"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX7523301.1"
                     /translation="MRAGGSDPVREAARLLAGRRNAVVLTGAGISVESGIPSFRGAQG
                     MWAKYDPMEYATLHAFMQSPRKVWEMLSEMVSVCGFASPNAAHKGLAALEGMGFVRAV
                     ITQNVDGLHQAAGSRRVIEYHGNLDELICVYCWKRYPTRERWTPGVVPLCDCGEILKP
                     NVVLFGEPIPWMPQEQAEEEARTCGVLVVVGTSAQVSPACDIPRVAKESGAAVVEINP
                     EPTSLTASVTDIHLRGSASEILRRLLKELSPGDEAPASAPSGH"
     gene            7507..8013
                     /locus_tag="VF357_03800"
     CDS             7507..8013
                     /locus_tag="VF357_03800"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX7523302.1"
                     /translation="MKDRRGFTLIEIMIVIAIVGIGVTIAFSNLQLWVYKYNFSGFQR
                     EVLSCIKRARTLSVSSQLQHRVIFNLDNEAVTLERGNAGVNSNVWTSVSGGVMRQAGT
                     QIGSVTPTPGAATTSGTFALIFDPSSEVDSSRTAAVTQTDIGLVGKNPGDVATIRVFG
                     WTGKARIQ"
     gene            8010..8468
                     /locus_tag="VF357_03805"
     CDS             8010..8468
                     /locus_tag="VF357_03805"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7523303.1"
                     /translation="MTTGMRHAARGEEGFSLIEVLIALTILAVGLLSLALLQVTAIKG
                     NAGASKSTIATDLAQTKLELFRRDAWIDIANSTNYTTFGGGTIPIYASVPADAGDNVI
                     VRGTNFYRIWRVTPNATNSLKTITVWSCWQDDQGNWHSVMLVTQRANVGS"
     gene            8471..9403
                     /locus_tag="VF357_03810"
     CDS             8471..9403
                     /locus_tag="VF357_03810"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEX7523304.1"
                     /translation="MRRNGTAGFSLIELMAAIVILGIAMTAVFSTFLFQQQSYTTQVR
                     VAEMQQNVRDAAEMISRDIRMCTYGIPSNVHIGGNDNEITSRYLTHVNSTSKPDEIYI
                     LYRYDGDAAYPPFDNVNLAAGANTVTLTSAAGFAAGDRVIVYDASNADLFLVTAVSGA
                     TLTLRGAAGATYSGASVPPARLARVRYVRYFIDNTTDSAHPTLMLDKRNGKPAQPLAD
                     DIEDLQFQYNMDMNNNGVISVPDGDLVDNNVAGTPQLVRQVHVYLLARSRIREKGWVE
                     TGNRNMADHLSGVANDGYRRRRTEMVLDVRNSSF"
BASE COUNT         1646 a         3009 c         3123 g         1672 t
ORIGIN      
        1 cccctcgaat gcggggtcgg ctcccagggc atacgatcga atggccacgg cgatcccgtt
       61 cgccgcctcc ttccggtacg acgacggcgt ttccggagag aggaccaggt tgcgcaggag
      121 aaggagagca cgctcggcgt ccccctcgtc gagagccgcg cgacccgcgg atacctgcct
      181 cgcggcgatc tccccgagga ctccctcgcg gatctccgcg accagttcac ggtatcggac
      241 gcccatctcc cgtgcccgcg caaggaccgt ccccaccttc gcctcgtcac cgccaaagaa
      301 gttcgagagt tcctctttcc tgaaatcttc cggttcgtac gcgtccacca ggatccggtc
      361 gaagtagggg aagtcggaat ctcgccccgc cccttccgcg atgtaccggt tcaggtcgct
      421 caagcttccc gccccttccg gcggggtcgc gttatacgcc gactcgcaga gcactgcgat
      481 catgtgcggg tagccgaaca cccattcgag gatcgggtgg acgatcgcct ggtacaccac
      541 atggggtcgg gaggcgtttt gttcgaggaa tgccttcggg agaaacacgc gcgggaacgt
      601 ccccggttcg gtccggaacc gcttcttccc ctcccagagg atctccccgt ctttctcgac
      661 gaggaggact tcgcatgccg gacgggcgtt ttcgcgacgg aacccttcgt ccggcccgag
      721 atcgaccacc cgcacggaga tcttccccct cgagaggagc gaggcgatcc gcgcgccggc
      781 gtttccggag atttccggag cgatctccgc caggatcgcc ggggacgggc gatccttgag
      841 gagcgagatc acctcgttca gtatcttttt cggcaacggc gttacatggc cggacttcat
      901 ctttttcctg aggatcgcgg caatctcccc gatctcgacg aaggtgatgg ggcggtctcc
      961 gggagccttc ttcgtgatcg gcggcggttt cttcccgtcc ggttcggccg gcgggaggat
     1021 tgtcaggaac gacgattggg cggcatcttt ttcgaggaaa cggccgaccc attcctggcc
     1081 ggagggcgag gcggcgacgg cgccgaacat cctggcgtcg ttgcgcaatc cctcctcgaa
     1141 cccgtgctcg aaaccgtgct tcatcgccag cagggcgtcc cgcgccgccg caagccgcgc
     1201 cgcgcgcaag tttcccgcgg actcctttcc aggagtcggg gcttcgagga tcgccttcac
     1261 ttcgggacgg gcgaagaggc gcgcaagttc ttccatccag ccgccggcca gaccgtccca
     1321 gtcccggcga ggcacggggc gctttcctgc ggcgaagtcg cgcgcggcgg cgaccgcgcg
     1381 cgggagggcc gcggaagccg gtacgaattc gtcggcccac ccgagcgaca ccgccgtgct
     1441 cccgtccacc ggttccccgt tgatgcagat gtccgccgct ttcgaggggc cgaccagacg
     1501 ggggaggcgc tgcataccgc cgtatcccgg gatgatcccc agtttcacct ccggctgtcc
     1561 gaggagggcg ttctcggtca cgatccggta atgcgcgctc atggcgagtt cattgccgcc
     1621 tcccagcgcg aagccgtcta caagggccac gacggggagg gccaggttct cgagctccgt
     1681 gaagacggag atgttcttcc acgcgagggc gtccacgact tccgcggttt tcccgtggaa
     1741 ctccctgaca tccgcgccgg cgacgaagga gcgcccggcc ccgttgagga cgatcgcctc
     1801 gacggtccga ccgccgatcg tgccgagaat ccccgcctcc cggacgacgg cggcgaattg
     1861 agaaaggagt gcctcgctca aggcgttgag cgaatcggga cgggtaaggg tgacgaccgc
     1921 cacatcgccg gccatccgca catcgacgaa tttccgggcc gccggatcgg ccgcctggac
     1981 gatttctccc cggaacgctt cctcgtagtc ggctaccgtc cgcccctggc ggttcgcctg
     2041 cacgagggcg acccccttcg gcttccgcat cgtcccgtcc agcatcatct gctgcaggcg
     2101 gggaaggccc ggaaggtccg taatctcgag gtcttcctgg tccatcgcga tctttcccgc
     2161 ggacaggaga cggatcacat cggcggcctg ggaagcccgg tacatgtggg agcccctgat
     2221 ggagaggtcg ctctttcccc gaaggacgac cgccccgcaa gggaagacgt ccttttcgtc
     2281 gaaatcccgc gcgacgaagg tgctgaagtg gagcgtgtcc tggtcgacgc ggtccacgac
     2341 gacgtcgggg gagacgcgag gaccgaacac tttccatagc gctttcccca gcgcctgcgc
     2401 gggacgggtg aagccggatg cgtattcggg gttgaggcca ccctccggag tcagcgggtc
     2461 cggcatatct tcggggaggg agaagccgtc gcggtcgagg atgtgggtcg gcggcactcc
     2521 catccgggaa agtttggcga tcccgcgctt ctcccgcgtt gcggaggcga tcacgacgac
     2581 ctccgcggtg cgtgcccgcg ccgccgcgac gaactccctc gcggaacggt ccgctcccca
     2641 gacgagcacc cgcctcccgg ggggcagtcc gagttcgtcg aagatcgcgg caggtttcgc
     2701 ccgccggaag aggacctgct tctgccgcat ccacacccag cgggcgtcca tgacgaaacg
     2761 gcgcccgtcg tggaagaacg tctccttgta ttcgcctttc aaccctttcc cggtggctcc
     2821 gaagaaggcg agcgatccgc cgggggagag gacggatatg agcaatggga aattttcccc
     2881 gccggtgtgt tcgaagatct tgtccaccgg ctttccgccg tttgcctcga agacggccct
     2941 ccggaacgga tcggtccgtt gctcccactc cgcgtacggc atccccgcgg ggatcacgcc
     3001 gaacgcctct ttcgggaccg agcggcggtc cacgaaggcg tcggccatcc cccgcgagat
     3061 cagatcccgc gccagttcgt ggttggagcc gacaaggagg acccgcgcgc cgagggattt
     3121 cgcgatctgg gctcccggga aactcgtccc ctttccgcct cccatcacga ggaccgcgtc
     3181 cccgggccgc accgagagcc gctccgccac cgcgcggtat gcggtcccat acgtcaggag
     3241 catggcggca agcctctccg gtgtgagatt ccgcaaagcc cctgtcaccg gcaccgcgcg
     3301 ctcctcgtcc acgacggcga actgggcgtt gaacccgtcg tgcgtctcgt atccccggat
     3361 gttccggccg gtccacgagt cgaggatgac cgcctggccg accttgaccg ccgaaacgcc
     3421 cgggccggcg tccaccacga tcccgagggc gtcgctcccc ggtacatgca cggacgccgt
     3481 gtcgcctttc ccgaacaccg gtaccggcaa cccgagtgcg gcgaagttcg tattgaaatt
     3541 ggggccggtc gccaggatcg ccacgaggac gcgcttcgcc tcgttcggtt tcaggcgcgg
     3601 tgcgggaaga gactccatgc gaacggaccg ggacggaggg ccgtattcgt cacggtggat
     3661 cgtgagcgcc agcatccgct gcggaagggg attccccagc gacggaggga gaccgagggg
     3721 ccaggaatct tcttgccgtg tgaatggatc ggacatgtcg gaatcctcct gctcataggt
     3781 gagaagcggg aatccttcag cgggacaagg gttcgtctgg ttcgatgcgc tttttcccac
     3841 gaggatttca tcgatcaaac agtattttgc gcttttcgga aaatgcccgc ggcggaaaga
     3901 tttcttagcg gcccgtttcg cgtattgacc ccggtcgcgt ttcgcgtata cgatgagggc
     3961 cgaaacggtg agacgtgcgt tgtggtcacc cgcaggatcg acccacgcga atttcttcac
     4021 caacggattc ggaatccggc acagcggaag cgcgcatcgg cgctcttcct gctgcggagt
     4081 gggggaaagc tccccggccg gccgaacgca gggggcggcg taacgctcga agattcacgg
     4141 acggccccac ggggccagca gaggagtcgt aggcatggca ttcggcacgg taaaatggtt
     4201 caacgacgcg aaggggttcg gctttatcac ggaagagggc ggggaggata tcttcgtcca
     4261 cttcagcgag atcaagggag aggggttccg gaccctcgcc gaggggcagc gggtggagtt
     4321 cgaggtcacc tcaggcccga aggggaaaaa ggcggcaaac gtccgcaagg cctgaacgat
     4381 acccgtcccc gccccgtgaa cgcggggcgg ggacggtgac atttccgcgg gggcgtcacg
     4441 aacgatgcgc atcggtgtcc cgcgagagac caagcccggg gagaaccggg tcgccattac
     4501 accattggcg gtccgggcac ttcgcgattt cggcactgag gtcgtcgtgg agaccggcgc
     4561 cgggacggtc agcggattcg aggaccgtga atacctcgaa tccggcgccg cgatcgtctc
     4621 gaaagcgtcg gaggcttggt ccgccgacct cgttgtgaag gtcaaggaac cgcttgcccc
     4681 cgagtttcct ttcctgtcgg aatcatccgc tctcttcgcc ttcctgcacc ttgccgcttt
     4741 accggggctc acgggagaac ttctggcccg tcgcgtaacg gccgttgcgt acgagaccgt
     4801 gacgtccgcc ggcgcgttgc cgatccttcg accgatgagc gaggtggcgg gaatcctttc
     4861 catccaggtt ggggcgaggg ggcttgaaaa ggggtgcggc gggcgcggga tactgctctc
     4921 cggggccgag ggatcgccgc cgggggatgt gatcgtgctg ggggcggggg ttgccgggcg
     4981 caacgccgcg cggatcgccg ccaggatcgg cgcgatcgtg acgattctcg acgtgaaccc
     5041 cgaaaaactg gcattggcga aacgtgaatg cgggggaaac gtgcgcacga tccggtcgac
     5101 ggcagaggcg gtaaacgatg cggtccggga ggccgatctc gtcgtttcga ccgtgctggt
     5161 ggcgggggag cgggcgccgc tcctcgttcc ccgggcgatg gtccgcggga tgcggaccgg
     5221 ggcggtggtg gtggatatcg ccatcgacca gggtgggtcg atcgaaactt cccgcccgac
     5281 gacacacgag gccccctact ttatagatga acgggtcgtc cactactgcg tgacgaatat
     5341 gcccgctgcc gtgccgcgca cctccacgcg ggggctcgtc gccgccacgc tgccatatct
     5401 tctgtcgttc gcgcggcaag gcgttgccgg cgcgttgcga ttggacgaag gccttctcgc
     5461 ggggctcaac acctgcggag ggcacgtcac ctgcgagggg gtggcgaagg cgttcgggaa
     5521 gccgttcctc cccccgaggg atgcgatccg gtaactccat tttccagcag cacccttgcc
     5581 agtgcgagga cctggcggct cgtttcttcc gggctcgccg agttgtcgat cacgtaatcg
     5641 gaagctccgg cctttcggtc agcgtccctt tgcgcacgga gcctcgcctc ggcctgactg
     5701 cgcgacatcc cgtcgcgggc ggcgagtcgt gaaatcgccg tttcccggtc gcacgcgacc
     5761 gagatcaccg cttcgaacag ccccgttttc cccgattcgt ggatgagggt cgcctccaca
     5821 acggcggcac gatgtccctc ccgttcgatc ccggagatcg cttccttcat cgcttcgagg
     5881 atggcgggat gggtgatcct ttccagtcgt tcgcgcgacg cggggtcgga gaagacgatc
     5941 tctcccaggc gcttcctgtc gatcgttccg tcgtcccgaa cgatcgcctt gccgaacgcc
     6001 cggacgacgg cgtcgtatgc ccgtcctccc ggggcggtca cttccttcga gatccggtcg
     6061 gcatcgagga ccgggatccc cgcctcgcgc agcatcgcgg ccaccgtgct tttccccgag
     6121 ccgatgtttc ccgtcaaacc gaatacccgc atgccgggga gtataccatc cgcgttgaca
     6181 acccgcccgg ggtcggtgta cgttcgagcg gggatgaacg gcggttcatt tcatccgaaa
     6241 tccagggggg gcagatggcg gagaccacgg tgaagggatt cttcgagggg ttatcggaca
     6301 agttgaacgc gaaaccggaa aaagtcgccg ggatgaattg cgtttaccag ttccgcgtta
     6361 ccgaagccgc ctggaacgtg aagttggcgg acgggaaggt tgccgtcgcg gaaggggagg
     6421 tgccttcccc gaactgcacc atcacgatgg cggaggccga tttcctcgac ctcgtttccg
     6481 ggaagttgaa cgggcagatg gcgttcctga ccgggaagtt gaaggtcgcg ggcgacatgg
     6541 ggctggcgct caaactgggg tcgttcatag ggtagcagga gttcccatgc gcgcgggcgg
     6601 gagcgacccg gtccgagagg cggcccgcct cctcgccggg cgtcgaaacg ccgtcgtcct
     6661 gacgggcgcg gggatctcgg tggagagcgg gatcccgtcg ttccgcgggg cgcaggggat
     6721 gtgggcgaag tacgacccga tggagtacgc cacccttcac gccttcatgc aatccccccg
     6781 caaggtatgg gagatgcttt cggagatggt ctccgtctgc ggctttgctt cgcccaatgc
     6841 ggcgcacaaa gggttggccg ccctcgaagg gatggggttc gttcgggctg tcatcacaca
     6901 gaacgtggac ggtctgcacc aggcggcggg ttcccggcgg gtgatcgaat accatgggaa
     6961 cctcgacgag ttgatctgcg tgtactgctg gaagcggtac ccgacccggg agcggtggac
     7021 gccgggggtc gttcccctgt gcgactgcgg cgagatcctc aagcccaatg tggtcctgtt
     7081 cggcgagccg atcccgtgga tgcctcagga acaggccgag gaagaggccc ggacctgcgg
     7141 cgtcctcgtg gtggtcggta cctcagcgca agtttccccc gcctgcgaca tcccccgggt
     7201 cgcgaaggag tccggcgccg cggtggtcga gatcaacccg gaaccgacgt cgctcaccgc
     7261 atccgtgacc gacatccacc tgcgggggag cgcttccgag atcctccggc ggctgctgaa
     7321 agagctctcc ccgggggacg aagcgcccgc atccgcacct tccggacatt aacttattta
     7381 atgtacatgt aaatattttt cgcggaattg ccgataacgt ttgccgggat gatgaaatat
     7441 cggcaactta cacgcaaacg tgtcgtggca cgggcattgc tttctccatg cccgggagga
     7501 tacggaatga aagatcgtag aggattcacg ctgatcgaga tcatgatcgt gatcgcgatc
     7561 gttgggatcg gcgttacgat cgccttctcc aacctccagt tatgggtcta caagtacaac
     7621 ttctcggggt tccaacggga agttctctcc tgtatcaagc gggcgcggac actatcggtc
     7681 tcatcccagc ttcagcacag ggtcatcttt aacctcgaca acgaggcggt gacgctggag
     7741 cggggaaatg ccggggtcaa ctcgaatgtc tggacgtccg tgtcaggtgg cgtgatgaga
     7801 caggccggga cccagatcgg cagcgtcacc cctacgccgg gggctgcaac aacgtcaggc
     7861 accttcgcac tgatcttcga tccctcgagt gaggtggaca gctcaaggac cgctgcggtg
     7921 acgcagacgg atatcggctt ggtggggaaa aaccccgggg acgtggcgac gatccgcgtg
     7981 ttcggatgga ccgggaaggc gaggatccag tgaccacggg gatgaggcac gccgctcgag
     8041 gcgaggaagg gttctccctg atcgaggttc tgatcgcatt gacgattctc gcggtggggc
     8101 tgctgtcgct ggcgctcctg caggtcaccg cgatcaaggg gaatgcgggc gcgtcgaagt
     8161 ccaccatcgc gacggacctg gcgcagacga agttggagct ttttcggcgg gacgcctgga
     8221 tcgatatcgc caattccacg aactacacga cgtttggcgg agggacgatc ccgatctacg
     8281 cgagcgtgcc cgccgacgcg ggggacaacg tcatcgtccg cgggacgaat ttctaccgga
     8341 tctggcgcgt gacaccgaac gccacgaact cgctgaaaac catcaccgtc tggtcctgct
     8401 ggcaggatga ccagggaaat tggcacagcg tgatgctggt cacccagcgg gcgaacgtgg
     8461 ggtcgtgacg atgcgaagga acggaacggc agggttctcc ctcatcgagc tcatggcggc
     8521 gatcgtgatc ctgggcatcg cgatgaccgc cgtgttcagc accttcctct tccagcagca
     8581 gtcatacacg acccaggtcc gggtggcgga gatgcagcag aacgtccgcg acgcggcgga
     8641 gatgatttcc cgggacatcc gcatgtgcac ctacgggatc ccctccaacg tccatatcgg
     8701 cggcaacgac aacgaaatca cctcccggta cctcacgcat gtcaacagca cctccaagcc
     8761 ggacgagatc tacatcctgt accggtacga cggcgacgcg gcgtaccctc ccttcgacaa
     8821 cgtcaatctg gccgcggggg ccaatacggt gacgctgacc agcgcggcgg gattcgccgc
     8881 cggggatcgg gtcatcgtct atgacgcgtc gaacgccgac ctgttcctgg tgaccgcggt
     8941 gtcgggcgcc acgctgaccc tccggggggc ggcgggcgcc acctacagcg gcgcgagcgt
     9001 cccgcccgct cggctcgccc gggtccggta tgtgcggtat tttatcgaca atacgaccga
     9061 ttccgcgcat ccgacgctca tgctcgacaa acggaacggg aaaccggccc agcccctggc
     9121 ggacgacatc gaagaccttc agttccagta caacatggac atgaacaaca acggtgtcat
     9181 cagcgtcccc gatggagatc tggtggacaa caacgtggcg ggtacccccc agctcgtccg
     9241 ccaggtgcac gtctacctgc tcgcccgctc gcggatcagg gagaaggggt gggtggagac
     9301 ggggaaccgc aacatggccg atcacctctc cggggtcgcc aacgacggct accgccgccg
     9361 gaggaccgag atggtgttgg acgtgaggaa ttcgagcttc tgacgggaga ggaccatgat
     9421 ttccgatcgc acaatacggc gggggcgtct
//
LOCUS       DASVJL010000185         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_590369, whole genome shotgun sequence.
ACCESSION   DASVJL010000185 DASVJL010000000
VERSION     DASVJL010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590369"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1158
                     /locus_tag="VF357_03815"
     CDS             118..1158
                     /locus_tag="VF357_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523305.1"
                     /translation="MTTFPGRFACTGVGSLPHLDPRAAVAQILSRFREIPYWPQLPRR
                     TPLEHMYPQFAGALPGASVVGERLTMKSGEALLPDAEAFYETFLSGDLSPFAVPAERA
                     AGLHALLEAGVGPFPAVKGQVTGPVSFGLMVCDREKKPVFYDPVGRDVLLKYLLRVAQ
                     WQASMLRRLSGTVILALDEPYLASVGSAIVSLPREEVIAALDEIFDGLPGVLCGIHCC
                     ANTDWGLVLASKVAYLSFDAYEYADSLLLYPEEISAFLARGGMLAFGVVPTGREAIEA
                     ETPESLADRMEGILDRYASRGISREAAVRASVITPACGLGTLPEESAERALRLTVELS
                     ALLRTRYGKSPP"
     gene            1155..1907
                     /locus_tag="VF357_03820"
     CDS             1155..1907
                     /locus_tag="VF357_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7523306.1"
                     /translation="MTLRIAVAGKGGVGKTTCSALLLRALALSGVRPLLAVDADPNAN
                     LHQLLSLPLPEGLGSLREEQRAAGVGAVPLTDRIASEVERRISEGDAVDLIAMGRGEG
                     PGCYCYVNNLLREALDRIEGGYRAVVMDNEAGMEHLSRRSVRRIDHLILVGDPSRRGT
                     SAVAALRDLAAELALPVGKTWLLLNRPYGDPGRAAPEDLGLPLFAALPHDPRLPSWEN
                     EGRSFLDLPPGESPAADAVADALRTCLLGSAA"
BASE COUNT          268 a          763 c          658 g          332 t
ORIGIN      
        1 gctgtaccac gacctctcct ccgatcccgg cttcatggac ctgtaccagc gcggactctt
       61 cctcccccac accgacgagg agaggtaccg ctccgtgctc gccatggagg caaaccgttg
      121 accacgttcc ccggacgctt cgcgtgcacg ggcgtgggat cccttccgca cctcgatccc
      181 cgcgccgccg tggcccaaat cctgtcccgc ttccgggaga tcccgtactg gccccagctc
      241 ccgcgccgga cccccctcga gcacatgtac ccgcaattcg ccggggccct ccccggggcc
      301 tcggtcgtcg gagagcgcct gacgatgaag agcggggagg cgcttcttcc tgacgcggag
      361 gcgttctacg agacgttcct ctccggcgac ctctccccct tcgccgtccc cgcggagcgc
      421 gccgcggggc tgcacgcgct gctggaagcg ggcgtcggtc cctttcccgc ggtgaagggc
      481 caggtgaccg gacccgtctc cttcgggctc atggtgtgcg accgggagaa aaaaccggtc
      541 ttctacgacc ccgtgggccg ggacgtcctg ctcaagtacc tgctccgcgt cgcccagtgg
      601 caggcttcga tgcttcgccg cctctccggg accgtgatcc tcgcgctgga cgagccgtac
      661 ctcgcttccg tgggctccgc catcgtctcg ctcccgcggg aggaggtgat cgccgccctc
      721 gacgagatct tcgacgggct gcccggcgtc ctgtgcggga tccattgctg cgcgaacacc
      781 gactgggggc tggtcctggc ctcgaaggtc gcctacctct cctttgacgc ctacgaatac
      841 gccgactcgc tcctcctcta cccggaggag atctccgcgt tcctcgcccg cggcggcatg
      901 ctcgccttcg gagtcgttcc cacggggcgc gaggcgatcg aggccgagac gccggaatcg
      961 ctcgccgacc ggatggaggg gattctcgac cggtacgcct cccgggggat ttcacgggaa
     1021 gcggcggtcc gcgcctccgt gatcaccccc gcgtgcggcc tcggcacctt gcccgaggaa
     1081 tccgcggagc gcgcgctgcg gctgaccgtg gaactctcgg ccctgctgcg gacccggtac
     1141 gggaagagcc ccccgtgacg ctgcggatcg ccgtcgccgg caagggaggg gtcggcaaga
     1201 ccacctgctc cgccctgctg ctgcgcgcgc tggccttatc gggggttcgt ccccttctcg
     1261 ccgtggacgc cgaccccaac gcgaacctgc accagctcct ctccctgccc ctccccgaag
     1321 ggctcggttc cctccgggag gaacagcggg cggcgggcgt cggtgcggtc cccctcacgg
     1381 accggatcgc ctccgaggtc gagcggcgga tatccgaagg ggatgcggtg gatctcatcg
     1441 cgatggggcg cggcgagggg ccgggatgtt actgctacgt gaacaacctg cttcgcgaag
     1501 cgctcgaccg gatcgagggc ggctaccggg ccgtcgtgat ggacaacgag gcggggatgg
     1561 aacacctctc ccgccggtcc gtgcggcgga tcgatcatct catcctggtg ggcgaccctt
     1621 cccggcgcgg cacctccgcc gtggcggccc tgcgggacct ggccgcggag ctcgccctgc
     1681 ccgtcgggaa aacgtggctt ctcctgaacc gcccgtacgg cgaccccggc cgcgcggcgc
     1741 cggaggatct cggactccct cttttcgcgg cgctccccca tgatccacga cttccctcct
     1801 gggaaaacga ggggaggtcg ttcctcgacc ttccccccgg ggagtccccc gcggcggacg
     1861 ccgtcgcgga cgctctccgg acctgcctcc tcggatccgc ggcgtgaagc tccttttcgc
     1921 aacccagggg aagtacggcg agcggatcgc ggaatacatc gcggcgaacc gcccgcacgg
     1981 atgggagatc ctccgcctgc cccttccacg gagccttccc a
//
LOCUS       DASVJL010000186         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1060337, whole genome shotgun sequence.
ACCESSION   DASVJL010000186 DASVJL010000000
VERSION     DASVJL010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060337"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VF357_03825"
     CDS             complement(<1..1001)
                     /locus_tag="VF357_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7523307.1"
                     /translation="MRTLAAAVLLSLILGGCTVGPDYRRPDVETPRSWRIEEKDARDV
                     ADTAWWEQMNDPVLNDLVVAALRENKDVKIAAARVEEFSGRLSVSRGALLPQVGAGAS
                     AGRERLSNKGLPPIPATSATLGNLYQASAGATWEIDLFGRLRRATEAARADLLATEEG
                     KRATILSLVGSVVGEYVNLRDLDKQLEIARRTVQSREESYRIFRLRFQGGVISELELN
                     QAKSEYEQALATVPALERAVAQQEHALSALLGRNPGAIPRGAAIDHLGQPTVPAGLPS
                     DLLERRPDIRQAEQELIAANARIGVARAQYFPSISLTGALGGTSTDLSALFGGPART"
     gene            complement(1003..>1682)
                     /locus_tag="VF357_03830"
     CDS             complement(1003..>1682)
                     /locus_tag="VF357_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7523308.1"
                     /translation="INAAAAPGYSSGQAMAALEEVFAQTMPTDMGFDYLGMSFQEQKA
                     REGVPAAAVFGLSLLFVFLILAALYESWSLPFSVLLGTPIAVFGALAALMLRGMEFNI
                     YGQIGLIVLIGLAAKNAILIVEFAKKEYEGGKDPAEAALSGAKLRLRPILMTSFAFIL
                     GCVPLVAASGSGAVARRVMGTGVVGGMLAATCIAVFIIPMTFYVVEKLVHRASRGKTS
                     RRAGEGG"
BASE COUNT          273 a          578 c          582 g          249 t
ORIGIN      
        1 caggtgcgcg ccggtccgcc gaacagcgcc gaaagatccg tgctcgtccc cccgagcgcg
       61 ccggtgaggg agatgctcgg gaagtattgc gcccgggcga cgccgatccg cgcgttggcc
      121 gcgatgagct cctgttcggc ctgccggatg tccggccgcc gttccaggag atcggaaggg
      181 agcccggcgg gcacggtggg ctgtcccagg tggtcgatcg ccgctccgcg cgggatggcg
      241 cccgggttgc ggcccaggag cgcgctcagg gcgtgctcct gctgcgcgac ggccctttcg
      301 agggcgggaa cggtagcaag cgcctgctcg tattccgact ttgcctggtt cagctcgagc
      361 tcggaaatga cccccccctg gaagcggagc cggaaaatcc ggtaggactc ctcgcgggac
      421 tgcacggtcc gccgtgcgat ctcgagttgt ttgtcgagat cgcgcaggtt cacgtactcg
      481 ccgacgacgg agccgaccag ggagaggatc gtcgcccgct ttccctcctc ggtcgcgagg
      541 aggtcggcgc gtgccgcctc cgtggcgcgc cgcagccgtc cgaagaggtc gatttcccag
      601 gtggcgccgg cggacgcctg gtagagattt cccagggtcg cggaggtggc cgggatgggg
      661 ggcaacccct tattgctgag acgctcccga ccggcgctgg cgcccgcacc gacctgcggg
      721 agcaaggcgc cgcgcgaaac ggacagccgt ccggagaact cctcgacccg ggcggcagcg
      781 atcttgacgt ccttgttctc ccggagggcg gcgacgacga gatcgttcag caccgggtcg
      841 ttcatctgct cccaccaggc ggtgtcggcg acgtcccggg catccttttc ctcgatgcgc
      901 caggatcgcg gggtctcgac gtcgggacga cggtaatcgg gcccgacggt gcatccgccg
      961 aggatgagag aaaggaggac cgccgccgca agcgtacgca tgtcacccac cctcccccgc
     1021 acggcgggag gttttccccc gcgaagcgcg gtgcacgagt ttctccacga cgtagaacgt
     1081 catcgggatg atgaacacgg cgatgcaggt cgccgcgagc atgccgccga ccacgccggt
     1141 tcccatcacc cgccgcgcga cggcgcccga cccgctggcg gcgacgaggg gaacgcaacc
     1201 gaggatgaag gcgaagctcg tcatcaggat cggccgcaac cgcagcttcg cgccggagag
     1261 cgccgcctcc gccgggtcct tcccgccctc gtactccttc ttcgcgaact cgacgatcag
     1321 gatggcgttc ttcgccgcga ggccgatcag cacgatgagc ccgatctggc cgtagatgtt
     1381 gaactccatc ccacggagca tgagagccgc gagggcgccg aagacggcga tgggggtccc
     1441 caggagcacg ctgaacggca gcgaccagct ctcatacagc gcggcgagaa tcaggaatac
     1501 gaagaggagc gacaggccga aaacggcggc cgcgggcacc ccttcccgcg ccttctgctc
     1561 ctggaacgac atcccgaggt agtcgaagcc catgtcggtc ggcatggtct gagcgaagac
     1621 ctcctccagc gccgccatcg cctgccccga gctgtagccc ggcgcggccg ccgcgttgat
     1681 ct
//
LOCUS       DASVJL010000187         2574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1340758, whole genome shotgun sequence.
ACCESSION   DASVJL010000187 DASVJL010000000
VERSION     DASVJL010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340758"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1810)
                     /gene="msbA"
                     /locus_tag="VF357_03835"
     CDS             complement(47..1810)
                     /gene="msbA"
                     /locus_tag="VF357_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEX7523309.1"
                     /translation="MSLYRRMLEYTRPYLPRLLLAMLVMVCVSALSGSTAFLVKPILD
                     DIFIRKDAVLLTYIPLLVLAIYLVRGVLEFTQSYLMAGVGQRVVRDIRDHLYRHMQSL
                     SLSFYLRHPTGVLMSRVLNDVGLMQSAITDAATGLVKDIFTAAFLVFVVFYRDWKLAL
                     IALVVFPLAFWPIARFGRKLRRTSVKAQEITGGLSSHLQETISGAKLVKSFGAETYET
                     ERFATRNYELFRLSMKVVKVQAMTSPLSEMFAGVGAAAVIYYGGYSVVNGHSTPGNFF
                     SFLTALFMLYEPVKRLSRINNVVQQGLAAGGRVFEVIDTLPEVREVEGAGTLAPIRKE
                     IAFDRVDFRYVPGGEAVLKEIDFRVPAGTMVAIVGSSGAGKTTLVDLIPRFYDPQEGA
                     IRIDGIDVRSVKLASLREQIGIVSQHTILFNDTVRNNIAYGMPGAPMEKVVEAARRAN
                     AHPFIERMPEGYGTVVGEQGLRLSGGERQRLAIARALLKDAPLLILDEATSALDTESE
                     HVVQEALDALMRGRTTFVIAHRLSTVRNADVIFVVEDGRIVERGGHEELLSRESRYRS
                     FYLKQFEERKTDLPDGEAGRG"
     gene            complement(1807..>2574)
                     /locus_tag="VF357_03840"
     CDS             complement(1807..>2574)
                     /locus_tag="VF357_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523310.1"
                     /translation="GRAKTLARFTKGAVVLFPFEVEFLRASGVNAVFAGHPILDEIAP
                     FLDMPPDPERFGIPPGKRGVGLLPGSRPGEVAAHLPILLAAARLLHQRFPDLHFALPV
                     ARPSLREAIAREVAASGLPVALVDEARQLLFRGLEAAMAVSGTVTLELALLGTPAVIV
                     YRTSWLSYQVGRRLARVDCVGLPNIASGEKFLPELIQDDCTPPRIAGAVGEILADPAL
                     RARLRAKGLSLRERLRGPGPTEAVVSMLAKEAAGAWA"
BASE COUNT          441 a          870 c          867 g          396 t
ORIGIN      
        1 cttctcccca ctggtcatat ggcaccgtat gtgtggattg aagcacttag cccctgcccg
       61 cttcgccatc cgggagatcg gttttccgct cctcgaactg cttcaggtag aaggaccggt
      121 agcgggactc ccgtgagaga agttcctcgt gccccccccg ctcgacgatg cgcccgtcct
      181 cgacgacgaa gatcacgtcc gcattgcgga ccgtggaaag ccggtgggcg atgacgaagg
      241 tcgtgcggcc gcgcatgagg gcgtcgagcg cctcctgcac gacgtgctcc gattcggtgt
      301 cgagcgcgga agtggcctcg tcgaggatga gcagcggtgc gtccttcagg agcgcccggg
      361 cgatcgcgag ccgctggcgc tcccccccgg acagccgcag cccctgttcc ccgacgaccg
      421 tcccgtatcc ttcgggcatc cgctcgatga acggatgcgc gtttgcgcgg cgtgccgcct
      481 cgacgacctt ctccatcggg gcgccgggca tcccgtaggc gatgttgttc cggaccgtat
      541 cgttgaagag gatcgtgtgc tggctgacga tcccgatctg ctctcgaagg gaagcgagct
      601 tgacgctccg gacgtcgatc ccgtcgatgc ggatcgcgcc ctcctgcgga tcgtagaacc
      661 gcgggatcag gtcgacgagc gtggtcttcc ccgccccgct ggagccgacg atggcgacca
      721 tcgttccggc cgggacccgg aagtcgatct ccttcaggac cgcttccccc cccgggacgt
      781 accggaaatc gacgcgatcg aaggcgatct ccttccggat cggcgcgagc gttcccgcgc
      841 cctccacctc gcgcacctcc ggcagcgtgt cgatcacctc gaagacgcgg cccccggcgg
      901 cgagcccctg ctggacgacg ttgttgatcc gggagagccg cttcaccggc tcgtagagca
      961 tgaacagcgc ggtgaggaag gagaagaagt tccccggcgt gctgtggccg ttcacgacgc
     1021 tgtagccgcc gtaatagatg acggccgccg ctccgacgcc ggcgaacatc tcggagagcg
     1081 gggaggtcat cgcctgcacc ttcacgacct tcatgctgag ccggaacagc tcgtagttcc
     1141 gcgtggcgaa tcgctccgtc tcgtacgttt cggccccgaa cgacttcacc agcttcgccc
     1201 cgctgatcgt ctcctgcaga tgggacgaga gccctcccgt gatctcctgg gccttgacgc
     1261 tcgtgcgccg cagctttcgg ccgaaccggg cgatcggcca gaaggcgagc ggaaagacga
     1321 ccagggcgat cagcgcgagc ttccagtcgc gatagaagac gacgaacacg aggaacgccg
     1381 cggtgaagat gtccttgacg aggccggttg cggcgtcggt gattgcgctc tgcatcaggc
     1441 cgacgtcgtt caggacgcgg gacatcagca ccccggtggg gtgccgcagg taaaacgata
     1501 gcgacagcga ctgcatgtgg cggtacagat gatcgcggat gtcccgcacc acccgctgcc
     1561 ctacccccgc catcaggtag ctctgtgtga actcgagcac gccgcggacg aggtagatcg
     1621 ccagcaccag gagcgggata tacgtcagca ggacggcgtc cttccggatg aagatgtcgt
     1681 cgaggatcgg cttcacgagg aaggcggtcg agccgctgag ggccgacacg cagaccatca
     1741 cgagcatcgc gaggagaagc ctcggaagat acggccgggt gtactcgagc atccgccggt
     1801 agaggctcat gcccatgctc ccgccgcctc cttcgccagc atcgaaacaa ccgcctccgt
     1861 ggggcccgga ccgcggagcc gttcccgcag cgagagtccc ttcgcgcgca ggcgcgcccg
     1921 aagcgccgga tcggccagga tctccccgac ggcgccggcg atccgcggcg gggtgcagtc
     1981 gtcctggatc aattccggca ggaacttctc tcccgaggcg atattgggga ggccgacgca
     2041 gtcgaccctc gccagccgac ggccgacctg gtagctcagc caggaggtcc ggtagacgat
     2101 gacggccggc gtcccgagca gggcaagctc gagagtgacg gttcccgaaa cggccatcgc
     2161 ggcttccagc ccgcgaaaga ggagttgccg cgcctcgtcc accagcgcga ccggaagccc
     2221 cgaagccgcg acctcgcgcg cgatcgcctc ccgcagcgac ggccgcgcga cgggcagggc
     2281 gaagtggagg tcgggaaacc gttgatggag gaggcgcgcg gcggcgagca ggatcggtag
     2341 atgcgcggcg acttctcccg gccggcttcc ggggagcagg ccgaccccgc gcttccccgg
     2401 cgggatcccg aaccgttccg ggtcgggagg catgtccagg aacggggcga tctcgtcgag
     2461 gatcggatgc ccggcgaaga cggcgttgac gccgctcgcg cgaaggaact ccacctcgaa
     2521 ggggaacagg acgaccgcgc ccttcgtgaa ccgggcgagg gtcttcgccc ggcc
//
LOCUS       DASVJL010000188         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1811316, whole genome shotgun sequence.
ACCESSION   DASVJL010000188 DASVJL010000000
VERSION     DASVJL010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811316"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VF357_03845"
     CDS             complement(<1..293)
                     /locus_tag="VF357_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7523311.1"
                     /translation="MRYLRLPQGASSRAGPGRPDRRRRGDDRAADRRGGGHGVKETFY
                     ITTPIYYVNDVPHIGHAYTTIACDALARWHRMLGKRVFFLTGTDEHGEKVQKTA"
     gene            complement(176..940)
                     /gene="ricT"
                     /locus_tag="VF357_03850"
     CDS             complement(176..940)
                     /gene="ricT"
                     /locus_tag="VF357_03850"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEX7523312.1"
                     /translation="MDIAGIRLRGVCKTFHFDCTGVALQRGDYAVVQTERGASLGQVI
                     RRIDGHTPKGDKPTVGKVLRVASAEDMRAHQENARREAEAEAFCTARIAERGLPMKLV
                     RAEYLLDRSKVVCYFTADGRIDFRELVKDLAHELRTRIEMRQIGVRDEARAVGGVGPC
                     GKELCCATFLRDFEPITVKMAKDQKLSLNPAKLSGVCGRLMCCLIYEHDSYARQKGCV
                     TCASPKGPPPAPAPAAQTDDEEEMTARLTDEEEGTA"
     gene            complement(950..1930)
                     /gene="holB"
                     /locus_tag="VF357_03855"
     CDS             complement(950..1930)
                     /gene="holB"
                     /locus_tag="VF357_03855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX7523313.1"
                     /translation="MVEPLSAVIGQERAVALLRRYIGAGAVPQGLLFSGEEGLGKEKT
                     ARAFAAALLCRKPGKDGACGSCHDCRLLASGAHPNFLFITPETQFLRIDEIRALREEL
                     SLKAFSDRPRVAILCPADRMTPQAANALLKTLEEPPAGAHLILVAHRTSVLMPTIVSR
                     CQRIPFFPLSAGAVAEILSRHPDVGGEHPRPVIVLAAKVSGGSPGRALSLLPELAEER
                     EAWLALLTKLTPAAAVKLAESWKGEGDTPGRLAAPLSLVRDLSLLSSGGGADIMNEDL
                     REPLASAAVHPPPNGWDTAFRELLSISRMPPQPQKRLMLEAFFFGLHGKG"
     gene            complement(1924..2553)
                     /gene="tmk"
                     /locus_tag="VF357_03860"
     CDS             complement(1924..2553)
                     /gene="tmk"
                     /locus_tag="VF357_03860"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7523314.1"
                     /translation="MNKAHFVTFEGIEGSGKTTQLRRLSAHLAAKGVLHLATREPGGT
                     PLSDEIRALVLVPREETVSPETELLLYEAARAQHVRGLILPALLSGRVVLCDRFCDAT
                     TAYQAHARGLDAEQVERLNRFAAGGLVPGLTLLFDLPPEDGFARVKGRGAAKDRLERE
                     SLDFHRAVREGYLRLAGRDPGRVVRIDAAVPEDEVFRSVLRTVAQRFGW"
     gene            complement(2630..>3119)
                     /locus_tag="VF357_03865"
     CDS             complement(2630..>3119)
                     /locus_tag="VF357_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HEX7523315.1"
                     /translation="IGVTKEIKTSCGKLYVTINRDEKGIFEVFNQMGKAGGCAASQSE
                     AIGRLASLALRSGVQPGMIVKQLKGISCHLPSWGGNGGKILSCADAVSKAIEWYLENF
                     DAMFPGFPKPVAETARTTAKKASLPAEAEEIARGACPDCGSQIERQEGCLKCRSCGWS
                     EC"
BASE COUNT          467 a         1051 c         1092 g          509 t
ORIGIN      
        1 gcggtcttct gcaccttctc cccgtgctcg tccgttcccg tgaggaagaa gacccgcttc
       61 cccagcatcc ggtgccagcg cgcgagggcg tcgcaggcga tcgtcgtgta ggcgtgcccg
      121 atgtggggga cgtcgttgac gtagtagatc ggggtcgtga tgtagaacgt ctccttcacg
      181 ccgtgccctc ctcctcgtcg gtcagccgcg cggtcatctc ctcttcgtcg tcggtctggg
      241 cggccggggc cggcgcggga ggaggcccct tgggggaggc gcaggtaacg caccccttct
      301 ggcgggcgta cgaatcgtgt tcgtagatca ggcagcacat cagccgcccg cagaccccgg
      361 agagcttcgc cgggttgagc gacagtttct ggtccttcgc catcttgacc gtgatcggct
      421 cgaagtcgcg caggaaggtc gcgcagcaaa gctccttgcc gcacgggccg accccgccga
      481 ccgcgcgcgc ctcgtcgcgc accccgatct ggcgcatctc gatgcgcgtg cgaagttcgt
      541 gggccaggtc cttgaccaat tcccggaagt cgatccggcc gtccgccgtg aaatagcaga
      601 ccaccttgct ccggtcgagg aggtactcgg cccgaaccag tttcatcggg agcccccgct
      661 ccgcgatgcg ggcggtgcaa aacgcttccg cctcggcttc ccggcgggca ttctcctgat
      721 gggcgcgcat gtcctccgcc gaggctaccc ggaggacctt gccgacggtg ggcttatccc
      781 ccttcggcgt gtgaccgtcg atccggcgga tcacctggcc cagcgatgct ccccgctccg
      841 tctggacgac ggcgtaatcg ccgcgctgca gggccacccc cgtgcaatcg aagtgaaaag
      901 tcttgcagac gccacgaagg cggataccgg cgatgtccat tccctgcgct catcctttcc
      961 cgtgcaatcc gaagaaaaac gcctccagca tcagccgctt ctgcggctgc ggaggcatgc
     1021 gggagatcga caggagctcc cggaacgccg tgtcccaccc gttggggggg gggtggacgg
     1081 ccgcggacgc gaggggctcc cgcagatctt cgttcattat atccgctccg cccccggaag
     1141 ataagaggga cagatcccgg acgagcgaga gcggggcggc caggcgtccc ggggtgtccc
     1201 cctccccctt ccagctctcc gcgagtttca ccgccgccgc cggggtgagc ttggtgagga
     1261 gcgcgagcca cgcttcccgc tcctccgcga gctctggcag gagcgagagg gcacgccccg
     1321 ggcttccccc ggagaccttg gccgccagca cgatcacggg ccggggatgc tccccgccca
     1381 cgtcggggtg gcgggaaagg atctccgcaa cagcgccggc ggagaggggg aagaagggga
     1441 ttctctggca ccgggagacg atcgtgggca tcaggaccga ggtcctgtgg gcgacgagga
     1501 tcagatgcgc gccggccggc ggctcttcga gcgttttcag cagggcattg gccgcctgcg
     1561 gcgtcatccg gtcggcgggg caaaggattg cgacgcgggg gcggtcggag aacgccttga
     1621 gggagagttc ctcccgcagc gcgcggatct cgtcgatccg gaggaactgc gtttccggcg
     1681 tgatgaaaag gaagttcgga tgggcgccgg acgcgagcag ccggcagtcg tggcacgatc
     1741 cgcacgcccc gtccttcccg ggcttccggc aaaggagggc ggcggcgaac gcccgggcgg
     1801 tcttctcctt ccccaatcct tcctcgccgg agaagagaag tccctggggg accgcccccg
     1861 caccgatgta tcggcgcagg agggcgacgg cccgttcctg gccgatgacc gcggagaggg
     1921 gttctaccat ccgaaccgct gcgccaccgt ccggaggacg cttcggaaca cctcatcttc
     1981 cgggacggcg gcgtcgatcc ggacgacccg gccggggtcc cggccggcga ggcgcaggta
     2041 cccctcgcgc acggcgcggt ggaaatcgag ggattcgcgc tccagccggt cctttgctgc
     2101 gccgcgcccc ttgacgcggg cgaacccgtc ctcgggcggc aggtcgaaca ggagcgtcag
     2161 gccggggacg aggccgcccg cggcgaaccg gttcagccgc tccacctgtt ccgcgtcgag
     2221 cccccgcgcg tgcgcctggt acgccgtcgt cgcgtcgcag aatcggtcgc acaggacgac
     2281 ccggccggac aggagcgccg gaaggatgag cccgcggaca tgctgggccc gcgccgcctc
     2341 gtagaggaga agctcggttt cgggggagac ggtttcctcg cgggggacga gcacgagtgc
     2401 gcggatctcg tccgaaaggg gggtgccgcc gggctcgcgg gtggcgaggt gaaggacccc
     2461 cttcgcggcg aggtgcgcgg agaggcgccg gagctgggtc gtcttgccgg agccctcgat
     2521 gccttcgaag gttacgaagt gggctttgtt catgttcggt cgctcaaccg cggtcagcgg
     2581 gacactcctg gttctatcct gggaggaagg gatagagtgt cctgcgccac taacactcgc
     2641 tccagccgca ggagcggcac ttgaggcacc cctcctgccg ctcgatctgg ctgccgcagt
     2701 cggggcacgc gccgcgggcg atctcctccg cctcggcggg gagggacgcc ttcttcgccg
     2761 tggtccgggc ggtctcggcg acgggcttcg ggaagccggg gaacatcgca tcgaagtttt
     2821 cgaggtacca ttcgatcgcc ttcgacacgg cgtcggcgca ggaaaggatt ttcccgccgt
     2881 tgccgcccca ggaggggagg tggcaggaga ttcccttcaa ctgcttgacg atcatcccgg
     2941 gctgcacgcc ggatcgaagc gcgagggagg cgaggcgtcc gatcgcctcc gattgcgacg
     3001 cggcgcagcc gccggccttc cccatctggt tgaacacctc gaagatcccc ttctcgtccc
     3061 ggttgatcgt gacgtagagc ttcccgcagc tggtcttgat ctccttcgtg acgccgatc
//
LOCUS       DASVJL010000189         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_590896, whole genome shotgun sequence.
ACCESSION   DASVJL010000189 DASVJL010000000
VERSION     DASVJL010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590896"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /gene="hisB"
                     /locus_tag="VF357_03870"
     CDS             <1..746
                     /gene="hisB"
                     /locus_tag="VF357_03870"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX7523316.1"
                     /translation="SLPRREILRESLSRAEGFLTRSLRESVGIVNRLAPEHLSIVTRD
                     PWKGEAVMSREGQVERTTKETRIRLSLRLSGDGSGKIGTGIPFLDHMLTLFSRHGLFD
                     LTIAAKGDVEVDFHHVVEDVGICLGEAFRRALGDMKGIRRYGHAVVPMIEALAAVTVD
                     VSARPHLVYNSPLGNEKVGTFDVELVEEFLRAFAQSSGVCLHVNVAYGSNAHHTVEAV
                     FKARGRAMSAAVELDPRVKGVPSTKGVLG"
     gene            760..1413
                     /gene="hisH"
                     /locus_tag="VF357_03875"
     CDS             760..1413
                     /gene="hisH"
                     /locus_tag="VF357_03875"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX7523317.1"
                     /translation="MTRGSAIGVVDYGMGNLRSVSKALESLGFPTLVSGDPKVLSSCR
                     GIVFPGVGAFRDCMGNVVRQGLLPFLREYLASDRPFLGICVGMQLLFSGSEEFGRHEG
                     IGFFRGNVVRFPGDMPARDGGVLKVPHMGWNEVEVVGDPPALRGIPSGTYFYFVHSYY
                     AAASVPVDVVCRSTYGIPFAAAVSRGNRLAVQFHPEKSQSAGLRLLGNFGRLCAEAA"
     gene            1425..>1939
                     /locus_tag="VF357_03880"
     CDS             1425..>1939
                     /locus_tag="VF357_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX7523318.1"
                     /translation="MPFQVIPAIDIQGGKAVRLRQGRVEDATVFSDSPLEVARRFVAA
                     GASILHVVDLDGAFLGKPVNTEIICRIASGAGVPIQVGGGVRNYDSASRYFAAGVFRV
                     ILGTSIVRSPEEVTRITRAYPGKVAAGIDARDGRVAIRGWVEVTGVIAVELAHQIEEG
                     GVSCFIYTDIAR"
BASE COUNT          282 a          590 c          709 g          358 t
ORIGIN      
        1 gttctcttcc gcggcgcgag atcctgcggg agtctctttc ccgcgccgag ggatttctga
       61 cacgctccct ccgggagtcg gtcgggatcg tcaaccggct tgcgccggag cacctgagca
      121 tcgtgacccg ggacccgtgg aagggggaag cggtgatgag ccgggaaggc caggtcgagc
      181 gtaccacgaa ggagacccgg atcaggctct ccctgcggct ttcgggggat gggtcgggaa
      241 agatcggcac ggggattccc ttcctcgacc atatgctgac gctgttctcg cgtcacgggc
      301 tgttcgacct gacgattgcg gcgaaggggg acgtcgaagt cgacttccac cacgtggtgg
      361 aggacgtggg aatctgcctc ggggaggcgt tccgtcgggc cctcggggac atgaagggga
      421 tccgcaggta cgggcacgcg gtcgtgccga tgatcgaggc actggcggcg gtgacggtgg
      481 acgtctccgc gcgaccgcac ctggtctaca actcccccct cgggaacgag aaggtaggaa
      541 cgttcgacgt cgagctcgtg gaagagttcc ttcgggcgtt cgcccagtcg tcgggcgtct
      601 gcctgcacgt gaatgtcgcg tacggctcca acgcgcacca caccgtcgag gcggtgttca
      661 aggcgcgcgg gcgtgccatg tcggcggcgg tggagctcga tccgcgcgtc aagggcgtcc
      721 cctccacgaa gggggttctc gggtgagagg gagcgcttca tgacgcgggg atccgcaatc
      781 ggggtggtgg attacgggat ggggaacctg cgcagcgtga gcaaggccct cgagtccctc
      841 ggtttcccga ccctggtcag cggcgatccg aaggtgcttt cctcctgccg tgggatcgtt
      901 ttccccgggg tgggggcgtt ccgcgactgc atggggaacg tcgtccgcca ggggttgctg
      961 ccgtttcttc gggaatacct cgcctcggac cggccgttcc tcggcatctg cgtggggatg
     1021 cagctcctct tctccgggag cgaggagttc ggtcgccacg aggggatcgg cttcttccgc
     1081 gggaacgtgg tccggttccc cggcgacatg ccggcccggg acgggggtgt gctcaaggtg
     1141 ccgcacatgg ggtggaacga ggtggaagtt gtcggcgatc ctccggccct ccgcggaatc
     1201 ccttccggaa cgtacttcta cttcgtgcat tcgtattacg ccgccgcttc ggtcccggtg
     1261 gacgtggtgt gccgttcgac gtatgggatt ccgttcgccg ccgcggtgag tcggggaaac
     1321 cgcctcgccg tccagttcca ccccgagaag agccagtccg cgggtctgcg gctcctcggg
     1381 aacttcggcc gcctttgcgc ggaagccgca taagaggata catcgtgccg ttccaggtca
     1441 ttccggcgat cgacatccag ggtgggaagg cggtccggtt gcgccaggga cgcgtcgagg
     1501 atgccacggt attctcggac tccccgctgg aagtggcccg ccggttcgtc gccgcggggg
     1561 cgtccatcct ccacgtggtg gatctggacg gggcgtttct cggcaaaccg gtaaacacgg
     1621 agatcatctg ccgcatcgcc tccggggcgg gcgtgccgat ccaggtggga ggcggcgtgc
     1681 ggaattatga ttcggcgtcc cggtacttcg ccgcgggcgt gttccgagtg atcttgggga
     1741 cctcgatcgt gcgcagcccc gaggaggtca cccggatcac ccgcgcctac cccggaaagg
     1801 tggcggcggg gatcgacgcg cgcgacggtc gcgtggcgat ccgcggatgg gtggaggtga
     1861 cgggggtcat cgcagtggag ctcgcgcacc agatcgagga gggcggcgtc tcctgcttca
     1921 tctacaccga catcgctcg
//
LOCUS       DASVJL010000190         4396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1529931, whole genome shotgun sequence.
ACCESSION   DASVJL010000190 DASVJL010000000
VERSION     DASVJL010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529931"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VF357_03885"
     CDS             complement(<1..530)
                     /locus_tag="VF357_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7523319.1"
                     /translation="MDVFETMKSRQSIRKFRKDPVPREDILRMAEAASWAPTAGNSQN
                     FRFIAVQERETIHRMRGIVDEIVSRFTGTEVAGEGKATYQNLFAFAPAVICVIGTPYE
                     SATDRALREKDPERYKARRFQVNPGLQGISAGITQFILAACALGYGTCWMTGPLIAKQ
                     ELESALSVRHPEELLA"
     gene            complement(623..976)
                     /locus_tag="VF357_03890"
     CDS             complement(623..976)
                     /locus_tag="VF357_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523320.1"
                     /translation="MPRCVIITFAAVAALLSCAGCIVTTSTYDVKVREVEALRDAYAS
                     SNREASRLAGEVEALSKEVAGQKAVLQELSTRADECEGALARGKRESADRDRESAELR
                     KKTGTKEDAPARPTE"
     gene            1081..2751
                     /locus_tag="VF357_03895"
     CDS             1081..2751
                     /locus_tag="VF357_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7523321.1"
                     /translation="MPSAPDDLFEQFLALGYVAPDDLKEMKERSSALGIPLAEATLLA
                     DRLHSDARAWILSESLGIPFLEVEPDSISLDLAHLFPEALARENRIVPIAREGDRVTV
                     AVTDPFCHGAFAVLEEMTGLSLRMVVCPGRTIGGILARFYPDPSGFEPYDLGEGSISR
                     EEAEKWLSEGGKNRVCRQILLHAASRGMSGVRMSPVGRDVVIEGRSEEKHVRLLSFPL
                     RFRKPLLDAFLELAGVPGGSDLPQETVFHLESGTGVVAFQASFLRGLSGPEVIVKILP
                     DLRAGIPLDSVGFNPGQLEITEKVLRQGNGLFLVSSPGPEGVSTTLFSMLREAYRPGL
                     RVITVEERHRFRNEGYIQLDRRQAVERYSGDWSRLAESLEPDILMIEHLPDPAALADL
                     LHLAQGGPLVLCGIRRFNFDRALRTLLTLDVDPFILAHVMRLVLHQRLVKLLCMECRR
                     PVPAKPSLRMVGERYRGELERIVADALFYLPSGCPKCGGTGFSGRMALIELIPFTPGV
                     QNIVASEGMLEEKLSRLMEEDLYSAAQSVQEMLSRGMVTYEDVAPFFR"
     gene            2812..>4396
                     /locus_tag="VF357_03900"
     CDS             2812..>4396
                     /locus_tag="VF357_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7523322.1"
                     /translation="MKETTLHRMYLNRIAEGGDSVRYMVPGDHGYTPVTYRQVGDAAR
                     EIGLGLMALGLSRGDRVAILATTRLEWCLADIGSTLGGFVTVPIYPSNLPDQVEYILA
                     HSRARAVFVEDEPQYNKVAGVRSRLPHLSSVLMMTGGVEGKEGAMTLSAVRERGKAHG
                     SSNPGALEARAEEILPTDDLTIIYTSGTTGPPKGVVTRHSNYAFNVTSALKAVNVPRG
                     SMFLQFLPLAHSLGRLEHFLTFDAMAVSSFARSLQTVAEDLAMVRPEIMVSVPRLYEK
                     FYARVLAKVEEDGGLKKAIFEWALGVGREASRCRQQGGEPQGLLALKNGIADRLVFRK
                     IRARMGGRLRFFISGGAPLSREIAEFLHAIGVLILEGYGLTETSTVTTVNRLERYKFG
                     TVGKALPGTELRIAADGEILVRGSHIFREYFNDPAATREAIEPDGWFHTGDIGALDEE
                     GFLRITDRKKDIIVTSGGKNIAPQNLENLLKNDRYISQAFVFGDRKKYLTALLTLAPE
                     EIVVWAARQGIPNRSVEALA"
BASE COUNT          705 a         1459 c         1438 g          794 t
ORIGIN      
        1 atcgccagca gctcttcggg atgccggacg gagagtgccg attcgagctc ctgcttcgcg
       61 atcagcggac cggtcatcca gcaggtgccg taccccagcg cacaggccgc caggatgaac
      121 tgggtgatcc cggcggaaat gccctgaagc ccggggttca cctggaaacg acgcgccttg
      181 taccgctcgg ggtccttctc gcggagcgcc ctgtccgtcg ccgattcgta gggagtgccg
      241 ataacacaga tcacggcggg ggcgaaggcg aacaagttct ggtaggtcgc cttcccctcc
      301 ccggcgacct cggtcccggt gaaccgcgag acgatctcgt cgacgattcc ccgcatccgg
      361 tggatcgtct cccgttcctg gaccgcgatg aaccggaagt tctgcgaatt tcccgccgtg
      421 ggggcccaag aggcggcctc cgccatccgc aggatgtctt ccctcggaac cgggtccttc
      481 cggaacttcc ggatgctttg ccggcttttc atagtctcga agacgtccat tcgattcctc
      541 ctgttgctcg gtcgggtcat tccaagcccg agtaccccag ggagcgtact tcgtacggga
      601 gtggggcggc cggttattta tactactccg tcggtcgcgc gggggcgtct tccttcgtcc
      661 ctgtcttttt ccggagctcc gcggattccc ggtcgcggtc ggccgattcc cgcttccccc
      721 gcgccagtgc cccttcgcac tcgtccgccc gggtggaaag ctcctggagg acggcttttt
      781 gtccggcgac ctccttcgag agggcctcga cctcaccggc cagcctcgat gcttcccggt
      841 tcgacgaggc gtacgcatcc cggagggcct cgacctcgcg caccttcacg tcgtaggtcg
      901 acgtcgttac gatgcagccg gcgcaggaaa gtagcgcggc cactgcggca aacgtgatga
      961 tgacgcaacg gggcaaaggc ggttctccgt ggaaggtagt atccgctacg ggcaataatg
     1021 attttacttt ccggcacctt tttaataaag ttgaaaataa acacgggagg gctacgcccc
     1081 gtgccatccg cccctgacga tctcttcgaa caattcctcg cgctcggcta cgtcgcgccc
     1141 gacgatctga aggagatgaa ggagcgttcc tcggcccttg ggatcccgct ggccgaggcc
     1201 accctccttg cggaccggtt gcactccgat gcgcgcgcat ggattctctc ggaatccctc
     1261 gggatcccgt ttctcgaggt cgagcccgac tccatttccc tcgatctcgc ccacctgttc
     1321 cccgaagcgc tggcccgcga gaaccggatc gttccgatcg cccgggaagg ggaccgggtg
     1381 acggtggccg tgaccgaccc gttttgccac ggggcattcg cggtcctcga agagatgacc
     1441 gggctttccc tgcgcatggt ggtgtgcccc gggaggacga tcggagggat ccttgcccgc
     1501 ttctaccccg acccgtccgg cttcgagccg tacgatctcg gggagggctc gatttcccgg
     1561 gaggaggccg agaagtggct ctcggagggg gggaagaatc gtgtttgcag gcagatcctg
     1621 ctccacgcgg cgtcccgcgg gatgtcgggc gtccggatgt cccccgtcgg ccgcgacgtg
     1681 gtgatcgaag gccgctcgga ggagaaacac gtgcgcctgc tctccttccc cctccggttc
     1741 cggaaaccgc tcctcgacgc gttcctcgaa cttgcgggcg ttccgggcgg gtcggacctt
     1801 ccccaggaga cggtcttcca cctcgagtcc gggaccgggg tcgtcgcctt ccaggcgagt
     1861 ttcctgcggg gtctctccgg ccccgaggtg atcgttaaga ttctccccga cctgcgggcg
     1921 gggatccccc tcgattccgt cggcttcaac cccggccagc tcgagatcac cgaaaaggtc
     1981 ctgcgccagg gaaacgggct cttcctcgtg tcttcccccg ggcccgaggg ggtctccacc
     2041 accttgttct ccatgctccg cgaggcgtac cggcccggcc ttcgtgtaat caccgtcgag
     2101 gagcgccacc ggttccggaa cgaggggtat atccagctcg accggcggca ggcggtggag
     2161 cggtattccg gcgactggtc ccgcctggcg gagtcccttg aacccgacat cctgatgatc
     2221 gagcacctgc ccgatccggc ggccctcgcc gacctgcttc acctcgcgca aggcgggccc
     2281 cttgtgctgt gcggcatccg gaggttcaac ttcgaccggg cgcttcgcac gcttctgacc
     2341 ctagacgtgg acccgttcat cctcgcgcac gtcatgcggc tcgtcctgca ccagcggctg
     2401 gtgaagctgc tctgcatgga atgccgtcgg ccggtccccg cgaagccctc cttgcggatg
     2461 gtgggggagc ggtaccgtgg cgagctggag cggatcgtcg cggacgcctt gttctacctt
     2521 ccctccgggt gtccgaagtg cggtgggacg ggcttctccg gccggatggc gctcatcgaa
     2581 ctgatcccct tcacgccggg ggtgcagaac atcgtcgcgt cggagggtat gctcgaagag
     2641 aagctgtcgc ggctcatgga ggaggattta tactccgccg cgcaatcggt gcaggagatg
     2701 ctcagccgcg ggatggtaac ctacgaggac gtagcgccgt tcttccgctg aaaagcggtc
     2761 ggaacggagt acggtcgagc tcgggaagat aaatggaagg cgaggggtgc gatgaaggag
     2821 acgactcttc accggatgta tctgaaccgg atcgccgaag ggggagattc ggtccggtac
     2881 atggtcccgg gcgatcacgg ctacacgccg gtgacgtacc ggcaggtcgg cgacgccgcg
     2941 agggagatcg gtctcgggct gatggcgctc ggcctgtcgc ggggggaccg cgtggcgatc
     3001 ctcgcaacga ctcgtctgga atggtgtctt gcggacatcg ggtcgactct cggcggattc
     3061 gtcacggtgc cgatctaccc ctcgaacctc cccgaccagg tggaatatat cctggcccac
     3121 tcgcgcgcgc gggccgtatt cgtcgaggac gagccgcagt acaacaaggt cgcgggggtc
     3181 cggtcccgcc tgcctcacct ctcgagcgtc ctgatgatga ccggcggcgt ggaagggaag
     3241 gaaggcgcca tgaccctctc cgcggtgagg gagcggggga aagcgcacgg ctcctcgaac
     3301 cccggggccc tcgaggcgcg ggcggaggag atcctcccca cggatgacct cacgatcatc
     3361 tacacctccg gcaccaccgg ccccccgaaa ggggtcgtga cgcgccacag caactacgcc
     3421 ttcaacgtga cctcggcgct gaaagcggtc aacgtccccc ggggatcgat gttcctccag
     3481 ttcctccccc tggcgcactc cctcggacgc ctggagcact tccttacctt cgacgcgatg
     3541 gccgtctcct ccttcgcccg ttccctccag acggtcgcgg aggatctcgc gatggtccgc
     3601 ccggaaatca tggtgagcgt accccggctc tacgagaagt tctacgcccg cgtgctggcg
     3661 aaggtcgagg aggacggggg gctgaagaag gcgatcttcg agtgggccct aggggtcggc
     3721 cgcgaggcgt cgcggtgccg ccagcagggc ggagagccgc aggggctcct cgccttgaaa
     3781 aacgggatcg cggaccggct ggtcttccgg aagatccggg cgcggatggg cggaaggctg
     3841 cgcttcttca tctcgggggg ggcgcccctg tcccgggaga tcgccgagtt cctgcacgcc
     3901 atcggcgttc tcatcctcga ggggtacggg ctcacggaga cctccacggt aacgacggtc
     3961 aaccgcctcg agcggtacaa gttcggcacc gtggggaagg cgctccccgg cacggagctc
     4021 cgcatcgccg cggacggcga gatcctcgtg cgtgggtcgc acatcttccg cgagtatttc
     4081 aacgaccccg ccgccacccg ggaggcgatc gaaccggacg gctggttcca cacgggggac
     4141 atcggggccc tcgacgagga ggggttcctc cgcatcaccg accggaaaaa ggacatcatc
     4201 gtcacctccg ggggaaagaa catcgccccc cagaacctgg agaatctcct caagaacgac
     4261 cggtacatca gccaggcgtt cgtgttcggc gaccggaaga agtatctgac cgcgctcctc
     4321 acgctcgccc ccgaggagat cgtcgtctgg gcggcccggc aggggatccc gaatcgcagc
     4381 gtcgaggcgc tggcga
//
LOCUS       DASVJL010000191         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1530063, whole genome shotgun sequence.
ACCESSION   DASVJL010000191 DASVJL010000000
VERSION     DASVJL010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530063"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VF357_03905"
     CDS             complement(<1..302)
                     /locus_tag="VF357_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX7523323.1"
                     /translation="MGVFRKLFIANRGEIACRAIRPARELGIPVVVGYSDCDALSLHV
                     KLADEAVRLGPSPASMSYLDIEAVLRAAKDSGCDAVFPGYGFLAENPDFAEAVEREG"
     gene            complement(313..1209)
                     /locus_tag="VF357_03910"
     CDS             complement(313..1209)
                     /locus_tag="VF357_03910"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX7523324.1"
                     /translation="MSQTTVVETSLSGLKLLGRGKVRDIYDVDGQLLLVASDRLSAFD
                     VVMPDGIPGKGKVLTQISAFWFRMLEDIVPNHMISIDVDAFPPEARVHAETLRGRSML
                     CRKAKPFPVECVVRGYLSGSGWAEYRQKGEVCGIPLAKGLRESDRLPEPVFTPATKEE
                     KGKHDENISFERMVRIVGKETAEKVRSIVVGLYEKAAAYALSKGIIIADTKFELGTVD
                     DRLILIDEALTPDSSRFWPVAGYQPGGPQKSFDKQFVRDYLLTLPWNKTAPGPRLPAD
                     VVEKTALKYREALTILTGKDIG"
     gene            complement(1287..3134)
                     /locus_tag="VF357_03915"
     CDS             complement(1287..3134)
                     /locus_tag="VF357_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523325.1"
                     /translation="MRHGQDDFFMDDHVGTRGIDRRLLARLLRYVLPHRRLLAAALAA
                     LLCGTACQLAGPYLIKVLIDRNVMTGQLSGMGRLVLLYLAALVGAMGFVYLQMYAVSV
                     LGQRVILALRREMFARMQRLPVVFFDRTPTGRLMTRLTSDVEALQELLSSGLVSTIGD
                     VAVLAGTAAILLWMDPGLALVTFAVLPVLILFVELLKKRIREANREMRRKLARMNAFL
                     QEHVTGVAVVNAFGMEGKSVRRFDALNEEYAAESVRLTNLYSVYFPGVDMLASIAVAM
                     LLWRGGVGHLSGTVTFGTLVAFLEYARRFYDPIRDMSDKYNILQSALASSERIFRLLD
                     VEISPEYAEAVPEPAPDARPSPSPIPSGSDAVPAVEFRDVWFSYPRREGGGTAGGEEG
                     PPVLRGVTFVLEEGQTGAIVGATGAGKTTILSLLCRFYEIRRGKILLFGRDIREIPRE
                     ELRGSLALVLQDTFLFSGTVRENVAAGGEAVGAALSAIGVERFTREWGEGLATDVGER
                     GVRLSTGQRQMVSFARALAREPRVLLLDEATSSVDPVTEGRIQEAMAGILPGRTALVV
                     AHRLSTVLSADRIIVMHKGKVRESGTHGELMAAGGIYRRLHALQFEEGG"
     gene            complement(3131..>3344)
                     /locus_tag="VF357_03920"
     CDS             complement(3131..>3344)
                     /locus_tag="VF357_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523326.1"
                     /translation="FEGILAEKGERTVLFSTHRMASLSRCDRILVLAAGRIVEEGTHD
                     ALFSLRGAYFDLYSRQMLARELEESS"
BASE COUNT          584 a         1191 c         1057 g          512 t
ORIGIN      
        1 ccctcccgct ccaccgcctc cgcgaagtcg gggttctccg cgaggaaccc ataccccggg
       61 aagaccgcgt cgcagccgga gtccttcgcc gcgcgcagga ccgcttcgat gtcgaggtag
      121 ctcatcgagg cgggggacgg ccccaggcgc accgcctcgt cggcgagttt cacgtgcagg
      181 gacagggcgt cgcagtcgga ataccccacc accacgggga tccccagctc ccgagccggc
      241 cggatggccc ggcaggcgat ctccccccgg tttgcgatga agagtttccg gaagacgccc
      301 atgcgcttcg gcctacccga tgtcctttcc ggtgaggatc gtaagcgcct cgcggtattt
      361 cagggcggtc ttttccacca cgtccgccgg caggcgcggc cccggcgccg tcttgttcca
      421 cggcagggtc agcaggtagt cgcggacgaa ctgtttgtcg aagctcttct gcgggccacc
      481 gggctggtag ccggcgacgg gccagaaccg ggaggaatcg ggggtgagcg cctcgtcgat
      541 caggatcagc cggtcgtcca ccgtccccag ctcgaatttc gtgtcggcga tgatgatccc
      601 cttcgaaagc gcgtaggcgg cggccttttc gtacaacccg acgacgatgg agcgcacctt
      661 ctcggccgtc tcctttccca cgatccgcac catccgctcg aaggagatgt tttcatcgtg
      721 tttccccttc tcctccttcg tggccggagt gaaaacaggt tccgggagcc gatccgattc
      781 ccgcaaacct ttggcaagcg ggatgccgca cacttccccc ttctgcctgt attccgccca
      841 ccccgagccg gagaggtacc cccggacgac gcactccacc gggaacggct tcgccttccg
      901 gcacagcatg gagcggccgc gcaacgtttc cgcgtgcacg cgggcctccg gcgggaacgc
      961 gtcgacgtcg atggagatca tgtggttcgg gacgatgtct tcgagcatgc ggaaccagaa
     1021 cgcggagatc tgggtcagta ctttcccctt ccccgggatc ccgtccggca tcaccacgtc
     1081 gaacgcggag agccggtcgg aggccaccag cagcaactgc ccgtccacat cgtatatgtc
     1141 gcggaccttc ccgcggccca gcagtttcaa cccggaaaga ctcgtctcga ccaccgtggt
     1201 ctggctcacg ttgcggacct ccaagaaggt gtggttgaaa ttttgcaact ataccacacc
     1261 accgaactcc ccgggacagc caccggtcac ccgccctcct cgaactgcag ggcgtgcagc
     1321 cgccggtaga tcccgcccgc ggccatcagc tcgccgtggg tccccgactc ccgcaccttc
     1381 cccttgtgca tcacgatgat ccggtcggcg gacaggaccg tcgacagccg gtgggcgacg
     1441 acgagggcgg tccgcccggg gaggatcccc gccatcgctt cctggatcct tccctccgtc
     1501 acgggatcga cgctcgacgt cgcctcgtcc agcagaagga cgcgcggctc ccgcgccagc
     1561 gcgcgggcga acgacaccat ctggcgctgg cccgtcgaca gccgtacccc ccgctcccct
     1621 acatccgtcg ccagtccctc tccccattcc cgtgtgaacc gttccacgcc gatcgccgag
     1681 agcgcggccc cgaccgcctc gccgccggcc gcgacattct cccgcaccgt cccggagaaa
     1741 agaaacgtgt cctggaggac gagggcgagc gacccgcgaa gctcctcccg agggatctcg
     1801 cggatgtccc gcccgaacag aaggatcttc ccgcggcgga tctcgtagaa gcggcacagc
     1861 agggagagga tcgtcgtctt ccccgcgccc gtcgccccca cgatcgcccc ggtctgccct
     1921 tcctcgagga cgaaagtgac cccgcgcagg accggcggtc cttcctcccc cccggcggtc
     1981 cccccgccct cgcgccgcgg gtaggagaac cagacgtccc tgaactcgac cgcggggacg
     2041 gcgtcggacc cggaagggat cggggacggc gatggccggg cgtcgggcgc gggttcgggc
     2101 acggcttccg cgtattcggg agagatctcc acgtcgagga gccggaagat ccgctccgag
     2161 gaggccagcg cggattgcag gatgttgtac ttgtcgctca tgtccctgat cggatcgtag
     2221 aaccttcggg cgtattcgag gaacgccacg agcgtcccga aggtgaccgt gcccgagaga
     2281 tggccgacgc cgccgcgcca taaaagcatc gccaccgcga tcgaggcgag catatccacg
     2341 cccgggaagt agacggagta gaggttggtg agccggacac tttccgcggc gtactcctcg
     2401 ttcagggcgt cgaaccgcct caccgacttc ccctccatcc cgaacgcgtt gaccaccgcg
     2461 acccccgtaa cgtgctcctg gaggaaagcg ttcatccgcg cgagcttccg ccgcatctcc
     2521 cggttcgcct cgcggatccg ctttttcagc agctcgacga acagaatgag gacgggaagg
     2581 acggcgaagg tgacgagcgc aaggcccggg tccatccaga gcaggatcgc ggccgtaccc
     2641 gccagcaccg caacgtcgcc gatcgtcgat acgagaccgg aggaaaggag ctcctggagc
     2701 gcctcgacgt cggaggtcag gcgcgtcatg aggcgcccgg tcggcgtgcg atcgaagaag
     2761 acgacgggga gccgctgcat ccgcgcgaac atctcccggc gcagagcgag gatcacccgc
     2821 tgcccgagga ccgacacggc gtacatctgc aggtagacga atcccatcgc gccgacgagg
     2881 gccgcgagat acaggaggac aaggcgcccc atccccgaaa gttgaccggt catcacgttc
     2941 cggtcgatga ggaccttgat gaggtacggc cccgccagct ggcaggcggt cccgcagagt
     3001 agtgccgcca gcgccgccgc caggagacgc cggtggggaa gaacataccg caggagacgc
     3061 gcgaggagcc gccggtcgat cccccgggtt cccacgtggt cgtccatgaa gaaatcgtcc
     3121 tgtccgtgcc tcacgacgac tcctccagct cccgggcgag catctggcgg gagtacaagt
     3181 cgaagtaggc gccgcgcagg gagaaaaggg cgtcgtgcgt cccctcctcg acgatccggc
     3241 cggccgccag cacgagaatc cggtcgcacc gggagaggga ggccatccgg tgcgtgctga
     3301 aaaggaccgt ccgctccccc ttttccgcga ggatcccctc gaag
//
LOCUS       DASVJL010000192         9125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_28141, whole genome shotgun sequence.
ACCESSION   DASVJL010000192 DASVJL010000000
VERSION     DASVJL010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28141"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VF357_03925"
     CDS             <1..1117
                     /locus_tag="VF357_03925"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX7523327.1"
                     /translation="LFSEWQAEGTLVRDPLPALYYYEQEFTIPGKGTFVRKGFLGALK
                     LSPFGEGVVFPHERTLAKPKADRLALMRVTDAHMSPIFGLYSDPGDTVLKDLRAGMAA
                     APDLVAVDDLGVKHRVWTVTQPKAILGAVETMADKGVFIADGHHRYETALAFREEMRA
                     RHGVNPDAAYEHVLMFLCNMDDEGIVILPTHRGIYSLPDFSAAGFLAKVRANLPVETR
                     DGSPGDAMRAVEAAGRDRKAIGWSSGDNRFHMVTFPDLSVFCDRHLSKFPPQLRTLDV
                     VLLHGYLFEQLLGISPEAVTAGQCVKYYKDPAKATADLSSRAIQAAFFLNPVSVDEFR
                     DVSLSGHVLPQKSTFFYPKIGTGLLIFPVAADDRVPG"
     gene            1550..2275
                     /locus_tag="VF357_03930"
     CDS             1550..2275
                     /locus_tag="VF357_03930"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7523328.1"
                     /translation="MNLGIDPGRLIIRQPERGYRFSIDSVILAGFAAPLCRGAVLDLG
                     TGCGVLLLLLSRLTPGMLAGTGVELQEDLLDFARRNFRDNCPDGRLFAVPGDFRREIP
                     GIEPGSFDLVVSNPPYGRAGHGRRNPDPGKESARHEVTCALPELFAAASRYLSAAGRF
                     AFILPYPRLSEIEPCAAKEGLRVEFLRVVYPRDGAPPSRGLCCTVRGGSGTPRLLPPL
                     FLHEGQEKYCTEVERICRLFRAG"
     gene            complement(2280..2726)
                     /locus_tag="VF357_03935"
     CDS             complement(2280..2726)
                     /locus_tag="VF357_03935"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX7523329.1"
                     /translation="MRRKDAVPLSSILDAFLESLRIPHVAFLVSLRKKWTEIAGPLVS
                     RNAIPLSLRNGVLTIVVQNHAWAQELRMSKTTLIGKIRETVGERIPVSDIRFTVGSLA
                     SVEKAEAAPREEPPFPAGPDPEGLSAVADPETRESLRALHRRSGGR"
     gene            complement(2723..3010)
                     /locus_tag="VF357_03940"
     CDS             complement(2723..3010)
                     /locus_tag="VF357_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523330.1"
                     /translation="MTASAFPLLPQAPWALMALIGGAALTVNIPLGYAREGFRRFSVG
                     WFVCVHLSVPLIAWMRLSNHVSAWGIPAFVACAVLGQIAGGKVRRIRRGRP"
     gene            3091..3408
                     /locus_tag="VF357_03945"
     CDS             3091..3408
                     /locus_tag="VF357_03945"
                     /inference="COORDINATES: protein motif:HMM:NF023251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HEX7523331.1"
                     /translation="MHLEWYHTAERKNEGVELTRATEPVLILIFRGTHQVLSAEKRLK
                     GGGVPLRLIPVPRRLTSDCGLAIRIPLDRRDRAREILSGARLLPVSAHLPRKGGEYDP
                     VDL"
     gene            3620..5557
                     /locus_tag="VF357_03950"
     CDS             3620..5557
                     /locus_tag="VF357_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7523332.1"
                     /translation="MRNVLIAAGFLLLFASIPSFGVAADIPSPATAGGAAVNPDGTKD
                     GKSPGSAPVYAAIPPPAAVTPGVTAAVPAASVTKVPPAPAPSVPAQNLPAPAAVPAVS
                     LVAPPSEKAPDRVLSPGEVDLQVKKNLEVDPEEGEGMGEAFFASASMEVEKGKGGAFS
                     GITSPIGKFINYFQTRGRKKFELYLSRSGKYVGMMQKILVRYGLPEDLVYVALIESGF
                     STKAYSVAKAAGPWQFISETGRRYGLRIDWWTDERRDAEKSTHAAASYLRDLYGMFDS
                     WPLAAAAYNAGEGKIQRAVTRYKSDDFSELIRHGYLKQETKDYVPKMLAALTIAKDPD
                     KYGFGDVAYEAPLNLRTVTVPGGTDLATVARLLEVPVEAISDWNPELRRFCTPPNRER
                     YDLRLSVDAALLAEERMEEIRTQAKITFLQHNVRRGETLQALADRYKTTVPVLKELNG
                     LKRDSLGRTARLVIPVTGLMETETVPGTEVSPEHLTMAHMRVEEGSRRAHVKGGRRPA
                     EPAGDAVTVRKGDTLARLAKRHGVRTKDLASANGLKPTSKLKVGSDLVLPEPAGAEES
                     RTAQTAAPKVRGGTKSSASTGAAQDVRKRTMRYKVHKGDTLDQIARVYGVTADRLAER
                     NRMKKGKLLRQGDVLVIPLES"
     gene            5637..6719
                     /locus_tag="VF357_03955"
     CDS             5637..6719
                     /locus_tag="VF357_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7523333.1"
                     /translation="MGEAILRAGNPGRSGHALSIRSARDLFVARERLAELFGCVDSSR
                     FAFAENATVALNQAIKGVLRPGDHVVTTSVEHNSVMRPLRRMEEAGVRVTVVPAGEDG
                     VTEARDVIAAFRKATRLVVIVHASNVSGALQPVDAVVAAAHRRGILTLIDAAQTAGSL
                     PIDLSSLPVDLLAASGHKGLLGPQGTGFLFVREGVPIVPLIEGGTGSRSESDRQPEFF
                     PDALESGTQNSVGVAGLAVSLAWILRKGVGAIRRKEVILVDQLLHGLSRIPGVTVYGP
                     EDPARRGAAVSFRVEGMAPAEVGARLEKRSGVLVRAGLHCSPNGHRALGTFPAGTVRV
                     SPGPFTKRAEIATFLSALRKIRDPSA"
     gene            complement(6652..7176)
                     /gene="rfaE2"
                     /locus_tag="VF357_03960"
     CDS             complement(6652..7176)
                     /gene="rfaE2"
                     /locus_tag="VF357_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX7523334.1"
                     /translation="MIPRRSVQTLCARLRSEGKRIVFTNGCFDLLHAGHAQYLRRAAA
                     LGDVLLVGLNSDASVRRLKGEGRPLQRATDRAYLLASLSCVSYVTIFPEDTPARLIGE
                     VIPHVLVKGGDWKEKEIVGSDVVRAHGGTVKTIRFLPGRSTTSILARAADIRRKGPGS
                     SEGRKGTSRSPRAS"
     gene            complement(7173..7979)
                     /locus_tag="VF357_03965"
     CDS             complement(7173..7979)
                     /locus_tag="VF357_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HEX7523335.1"
                     /translation="MPVSPFLAAALQFRVDIADVPANRERAVRLIEEAAARGARLCVL
                     PEMWSTGFAEERLLPLSRTTPEVLHELRSLAARLKVVVAGSLPERVGRGVYNTLYVVN
                     ATGVVTGEYRKAHLFSPSGENRWFRRGVSAEVVPTDAGTVGPLICYDLRFPELSRKYF
                     LDGAGVLCVSSQWPSVRRDHWRILTVARAVESQAYVVAANAVGPSGPFRYRGDSVIVS
                     PDGERLASVGEEEGMALATIDPAVVLETRRRIPCLADRNTRAYRKTRRPA"
     gene            complement(8005..8712)
                     /locus_tag="VF357_03970"
     CDS             complement(8005..8712)
                     /locus_tag="VF357_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX7523336.1"
                     /translation="MAQWIGELASLRDLLPAASPDLSPSAIARWSAHTEAVCPRCGAF
                     EGSGPGPHPSPCGLCAAAPPPFDAARSLFAYAGDVRAAIVATKYAGRPFPVEAVALRL
                     HETLVGRWRDLIPDGTPPTVVPVPAHPWKYFRRGFNLPALIASRLSRRIGFPFDPLAL
                     SRTRERIPQARLSGILRHENVEGAFRVPRGRKVPPAILLLDDVYTSGATADSCARALK
                     SAGVDSIVVVTVARAVP"
     gene            complement(8768..>9125)
                     /locus_tag="VF357_03975"
     CDS             complement(8768..>9125)
                     /locus_tag="VF357_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523337.1"
                     /translation="EFPSDYDVNLFMGKFFLRLEMIDEGLEQLLKAETLGPERESVNI
                     LLAEAFRRRGRYESASLHYQRAFGYKRRYLIPFRCSSCDSSTIKWTARCPSCGIWNGY
                     AIDHGNREYAVSATLR"
BASE COUNT         1527 a         2964 c         3147 g         1487 t
ORIGIN      
        1 tcttttcagc gaatggcagg ccgaggggac gctggtccgc gacccgcttc cggccctcta
       61 ctactatgag caggagttca cgattcccgg aaaggggacc ttcgtgcgga aagggttcct
      121 cggggcgctg aaattatcgc cgttcggcga gggggtagtc tttccccacg agcggaccct
      181 tgcgaagccc aaggcggacc gtctcgccct gatgcgggtg acggacgccc acatgagccc
      241 catcttcggg ctctactccg accccgggga cacggtcctg aaagatctgc gcgcggggat
      301 ggccgccgct cccgacctgg ttgcggtcga cgatctcggc gtgaagcatc gcgtctggac
      361 cgtcacgcag ccgaaggcga tcctcggcgc cgtcgaaacg atggcggaca agggggtgtt
      421 catcgccgac gggcatcatc ggtacgagac cgctctcgcc tttcgtgagg agatgcgcgc
      481 cagacatggc gtgaacccgg acgcggcgta cgagcacgtg ctgatgttcc tctgcaacat
      541 ggacgacgag gggatcgtca tcctccccac ccatcgggga atctactccc tgccggattt
      601 ctcggccgcc ggcttcctcg cgaaggtgcg cgcgaacctg ccggtcgaaa cccgcgacgg
      661 gtcgcccggg gatgcgatgc gggcggtgga ggcggcgggc cgcgaccgga aggcgatcgg
      721 atggagttcc ggcgacaacc ggtttcacat ggtcacgttc cccgacctga gcgtgttctg
      781 cgaccggcac ctctcgaagt tccctccgca gttgcgcacg ctcgacgtcg tccttctgca
      841 cggctacctg ttcgaacagc tgttggggat ctctcccgag gccgtgacgg cggggcaatg
      901 cgtcaagtat tacaaggacc ccgcgaaggc gaccgcggat ctctcttcca gggcgatcca
      961 ggccgccttc ttcctgaatc ccgtgtcggt cgacgagttc cgcgacgttt ccctctccgg
     1021 gcatgtcctc ccgcagaagt cgaccttctt ctacccgaag atcgggacgg ggctgctcat
     1081 cttccccgtg gcggcggacg accgcgtacc ggggtaaccg ccgcgatttc caagtgcttc
     1141 gtttcatata tacgggaaca agtgagcatt ggggggtagg actccgggct cgtgcccccc
     1201 gtttttccta cggcgggggt accccggcag cgcggagctc gtaccggggc ggggggcttc
     1261 ctctatattc caccgattcc gccttccctt ccgtgggatc accctccctc gcttcgctca
     1321 ccggacgtcc ctgtccggtt cgctggcacc tcccttggct cccgccgtcc tggctccgcc
     1381 aaggtcagat gcccgctagg gaggggatcc cactccaggt gcggcgaggg atgctggata
     1441 ttctgttcgt aagggagggc tgccggaacg tagcgaagaa gggggtatca cgcggagata
     1501 aaatcgcctc cgtttacgtt accgtattta taagggggag cactaagtga tgaacctcgg
     1561 gattgatccg ggccggctga tcatccgcca gccggagcgg gggtaccgct tctcgatcga
     1621 ctcggtgatt ctcgcggggt tcgccgcccc gctctgccgc ggcgccgtcc tcgatctggg
     1681 gacagggtgc ggggtcctgc tgctgctcct gtcgcgcctc acccccggga tgcttgccgg
     1741 taccggcgtg gagctacagg aagacctgct cgatttcgcg cgccggaatt tccgcgacaa
     1801 ttgcccggac gggaggctgt tcgccgtgcc tggggatttt cgtagggaga tccccgggat
     1861 cgagcccggt tcgttcgacc tggtggtgtc gaatccgccg tacggccggg cgggacacgg
     1921 gcgtcgaaat ccggatccgg ggaaggagtc ggcgcggcac gaggtgacct gcgcgcttcc
     1981 ggagctcttc gcggcggcgt cccggtatct ttccgcggcc ggccggtttg ccttcatttt
     2041 gccgtacccg cgcctttccg agatcgaacc gtgcgccgcg aaggaggggt tgcgtgtgga
     2101 gttcctgcgt gtggtgtacc cgcgggacgg cgcgccgccg tcccgcggac tctgctgcac
     2161 ggtacggggc ggaagcggaa ctccccgtct cctccctccg ctgttccttc atgaggggca
     2221 ggagaaatat tgcacggagg tggagcggat ctgccgcctc ttccgcgcgg ggtagagtct
     2281 cacctccccc cggaacggcg gtggagggcg cgcaggcttt cgcgcgtctc cgggtcggct
     2341 acggcggaca gtccttccgg atcgggaccg gcggggaacg ggggctcctc gcgcggcgcg
     2401 gcctccgcct tctcgaccga agcgagggaa cccacggtga accggatgtc gctcaccggg
     2461 atcctttctc ccacagtctc ccggatcttc ccgatcagcg tggtcttgct catccggagt
     2521 tcctgcgccc acgcatgatt ctgcacgacg atcgtgagga cgccgttccg caacgacagg
     2581 gggatcgcgt tccgcgagac cagcggaccg gcgatctccg tccacttttt ccgaagcgaa
     2641 acgaggaagg cgacatgcgg gatccgcagc gactcgagga acgcgtcgag gatcgacgag
     2701 aggggaaccg cgtctttccg tctcacggac gcccccgacg gatccggcgg accttccccc
     2761 ccgcgatctg ccccagcacg gcgcacgcga cgaaggcagg gatcccccaa gcgctcacgt
     2821 gattggagag ccgcatccat gcgatgagag ggacggagag gtggacgcag acgaaccacc
     2881 cgaccgagaa tctccggaac ccctcccggg cgtaccccaa ggggatgttc acggtgagcg
     2941 cggcgccccc gatgagagcc atcagggccc agggcgcctg cggcaggaga gggaaggcag
     3001 aagcggtcat cttctgatta taaccgcgtc cgggggatga agcgacggcg cggatgcccg
     3061 catcggcgag cccacgacgg agagtcggcg atgcatcttg agtggtacca tacggcggag
     3121 cggaaaaacg aaggagtcga attgacccgc gcgacggaac cggttctgat cctcatcttc
     3181 cggggcaccc accaggtcct gtcggcggag aagcggctga aggggggtgg agtgccgttg
     3241 cgactgatcc cggtcccccg acgcctcaca tccgattgcg ggctcgcgat ccggatcccc
     3301 ctcgaccggc gcgaccgcgc ccgggagatc ctgtcgggag cacggctgct ccccgtttcc
     3361 gcccaccttc cccgcaaggg cggggagtac gaccccgtgg acctctgaga atcgccggaa
     3421 gaggtaaagt tctctctcgt tcggccgata agatagaacg gggccgacgg agttgccgcg
     3481 ccccacgagt ccgacggccg gttcccccaa gggcggtcct ccgagtccga agatgagtca
     3541 gactattgac attggtgaaa tcatcaaata aaatgcgcgt ttatattata tccagggggg
     3601 tttctttgag ggaagagtcg tgaggaacgt cctcatagcg gcgggattcc tcctgctgtt
     3661 cgcgtcgatc ccgtcttttg gggtggctgc ggacattcct tcgccggcga cggcaggggg
     3721 agcggcggtc aatcccgatg gcacgaagga cgggaaatcg ccgggctccg cgcctgtata
     3781 tgcagccatt cctcctccgg cggcggtcac gcccggcgtg acggcggcgg ttcccgccgc
     3841 ttccgttacc aaggttcccc cggctccagc cccgtccgtt cccgcgcaga atctccccgc
     3901 cccggccgcc gtccccgcgg tttctctcgt cgcgcctcct tcggaaaagg cccccgaccg
     3961 ggtcctctcc cccggcgagg tcgacctgca ggtgaagaaa aacctcgagg tcgatccgga
     4021 agagggagag gggatggggg aagcgttctt cgcctccgcc tcgatggagg tggagaaagg
     4081 gaaggggggg gccttctccg ggatcaccag cccgatcggg aagttcatca actatttcca
     4141 gacccgggga cgcaagaagt tcgaactgta cctgtcccgc tcggggaagt acgtcggtat
     4201 gatgcagaag atcctggtcc gctacggcct ccccgaggac ctcgtctacg tcgcgctgat
     4261 cgaaagcggg ttctctacca aggcgtactc cgtggcgaag gcggccgggc cctggcagtt
     4321 catctcggaa accggccgcc ggtacggcct ccgtatcgac tggtggaccg acgagcggcg
     4381 agatgccgag aagtcgacgc acgccgccgc ttcctacctt cgcgatctct acgggatgtt
     4441 cgactcctgg ccgctcgccg ccgccgcgta caacgccggc gaagggaaga tccagagggc
     4501 ggtcacccga tacaagtccg acgatttttc cgagctcatc cgtcacggct acctgaagca
     4561 ggagacgaag gattacgtcc cgaagatgct ggcggcgctg accatcgcca aggatcccga
     4621 caaatacgga ttcggcgacg tcgcctacga ggcgccgctg aatctgcgaa ccgtgacagt
     4681 gccgggtggg accgacctgg cgacggtggc ccgtcttctc gaggtaccgg tggaggcgat
     4741 cagcgactgg aacccggagc ttcggcggtt ctgcaccccg ccgaaccggg agcgatacga
     4801 cctccggctc tcggtcgacg cggcgctgct tgccgaggag cggatggagg agattcgcac
     4861 ccaggcgaag atcaccttcc tccagcacaa cgtccgcagg ggggaaacgc tgcaggcgct
     4921 ggccgatcga tacaagacca cggtcccggt cctcaaggag ttgaacgggc tgaagcggga
     4981 ctccctcggg cgcaccgcgc gcctcgtgat cccggtgacc gggttgatgg agacggagac
     5041 ggttcccggg accgaggttt caccggaaca tctcacgatg gcgcacatgc gggtggagga
     5101 gggaagccga agggcgcacg tcaagggagg gcggcgtcct gctgaaccgg cgggggatgc
     5161 cgttaccgtg cggaaggggg acacgctggc gcgcctcgcg aagaggcacg gggtccggac
     5221 gaaggatctc gcgagcgcga acgggttgaa gccgacatcg aaactgaagg tgggttcgga
     5281 cctcgtcctg ccggaacccg ccggcgcgga ggaatcgcgc acggcgcaaa cggcggcccc
     5341 gaaggtgcgg ggggggacga aatcctccgc ctccaccggc gcggcgcagg acgtccggaa
     5401 gcggaccatg cgctacaagg tgcacaaggg ggacaccctc gaccagatcg cgcgcgtcta
     5461 cggcgtcacg gccgaccgtc tcgcggaacg gaaccggatg aaaaaaggga aactcctccg
     5521 ccagggcgac gttctcgtca tcccgctgga gtcctgatct gcccggggcc gtcccgtaca
     5581 tatacctcga caacgcggcg acctccctgc caaaaccgcc cggtgtggcg aaggccgtgg
     5641 gggaagccat cctgcgggcc gggaaccccg gtcgctccgg acacgccctc tccatccgct
     5701 ccgcccgcga cctgttcgtc gcgcgggagc gtctcgccga gctgttcggg tgcgtcgaca
     5761 gttcccgctt cgctttcgcg gaaaacgcca ccgtcgccct gaaccaggcg atcaaggggg
     5821 tactccggcc gggcgaccat gtggtgacca cctcagtgga acacaactcg gtgatgcgcc
     5881 ccctgcgccg gatggaggag gcgggcgttc gcgtcacggt cgtgcccgcg ggggaagacg
     5941 gggtgacgga ggcgcgggac gtgatcgccg cgttccggaa ggcgacgcgc ctggtcgtga
     6001 tcgtgcacgc ctcgaacgtg tcgggggcgc tgcagccggt ggacgccgtt gtcgcggcag
     6061 cgcaccgccg cgggatcctg acgctgatcg acgccgccca gacggcggga tccctgccga
     6121 tcgacctttc ctccctgccg gtggacctgc tggccgcctc cggtcacaag gggcttctcg
     6181 ggccgcaggg aaccgggttt ctcttcgtcc gggagggggt gccgatcgtt ccgctgatcg
     6241 agggggggac ggggagccgc tccgagtcgg accgccagcc ggaattcttt cccgacgccc
     6301 tcgagtccgg gacgcagaac agcgtggggg tggccggtct cgccgtctct cttgcgtgga
     6361 tcctgcgaaa gggggtcggg gcgatccgcc gcaaggaggt catcctcgtc gaccaactgc
     6421 tccacggact gtcgaggatc ccgggagtga cggtctacgg gccggaggat ccggcgcgcc
     6481 ggggggcggc ggtgtcgttc cgggtggagg ggatggcccc ggcggaagtg ggggcgcggc
     6541 tggagaagcg gagcggcgtg ctggtgcggg cggggctgca ctgctccccg aacggtcacc
     6601 gcgccctcgg aaccttccct gccgggaccg tgcgcgtgag tccgggcccc ttcacgaagc
     6661 gcgcggagat cgcgacgttc ctttccgccc ttcggaagat ccgggaccct tccgcctgat
     6721 atcggcggcc cgcgcgagga tcgaggtcgt cgaccggccg ggcaggaacc ggatcgtctt
     6781 cacggtgccg ccgtgcgcgc ggaccacgtc cgaccccacg atctcctttt ccttccagtc
     6841 gccgcccttg acgagaacgt gcgggatcac ctccccgatc aaccgcgccg gggtgtcctc
     6901 ggggaaaatc gtcacgtagg aaacgcagga aagcgaggcg agaagatacg cccggtccgt
     6961 ggccctctgc aacggccgtc cttccccctt caggcggcgg acggaggcgt cgctgttcaa
     7021 ccccaccagg agaacgtccc ccagcgccgc cgcgcgtcga agatattgcg cgtgccctgc
     7081 gtgcaggaga tcgaagcagc cgttggtgaa tacgatccgc ttcccctcgc tccggaggcg
     7141 ggcgcagagg gtctgcacgc tccggcgggg gatcacgcgg gccgacgggt cttccggtat
     7201 gcccgggtgt tccggtccgc gaggcatggg atcctccttc gggtttccag gacgacggcc
     7261 gggtcgatcg tcgccagggc cattccttcc tcctcaccga ccgacgccag gcgttcgccg
     7321 tcgggggaga cgatcaccga gtcgcccctg tagcggaacg ggcccgacgg tccgacggcg
     7381 ttcgccgcga cgacgtacgc ctggctttcg accgcccggg cgaccgtcag gatccgccaa
     7441 tggtcacgcc ggacggaagg ccactgggac gacacgcaca gcactcccgc cccgtcgagg
     7501 aaatatttcc gggaaagttc cggaaaccgg agatcgtagc agatgagggg tcccacggtc
     7561 cccgcgtccg tgggaacgac ttccgcggag acccccctcc ggaaccacct gttctccccc
     7621 gagggggaga acaggtgggc cttccggtac tcgccggtga cgacgcccgt ggcgttgacg
     7681 acgtagaggg tgttgtacac tccgcgtccg acccgttccg gaagggaccc cgcgacgact
     7741 accttgagtc gcgcggcaag cgatcggagt tcgtggagga cctccggggt cgtccgggag
     7801 agcgggagaa ggcgttcctc cgcgaatccc gtgctccaca tttccgggag cacgcaaagg
     7861 cgtgcgcccc gcgccgccgc ctcttcgatg aggcgaaccg cacgctcccg gtttgcggga
     7921 acgtcggcta tatcgacgcg gaactgcagt gcggcggcga ggaacggcga aacgggcatc
     7981 ggggctccgt gcggcgcatc gggatcaggg gacggcgcgc gcgaccgtca cgaccactat
     8041 agagtcgacg ccggcgcttt tcaaggcgcg ggcgcacgaa tccgccgttg cgccggaggt
     8101 gtacacgtcg tcgagcagaa ggatcgccgg cggaaccttg cggcctcgcg gtacgcggaa
     8161 agccccctcg acgttttcgt gccggaggat cccggaaagc cgcgcttgcg gaatccgctc
     8221 cctggttctc gaaagcgcca gtggatcgaa cgggaaaccg atgcgtctcg aaagtcggga
     8281 agcgatcagc gcagggaggt tgaatccccg ccggaaatat ttccagggat gggccggtac
     8341 cggaaccacg gtcggaggag tcccgtccgg gatcaggtcc ctccatcgcc ccacgagggt
     8401 ctcgtggagg cgcagggcca cagcttcgac cgggaacggg cgacccgcat atttcgtcgc
     8461 gacgatcgcg gcgcgaacgt ctcccgcgta ggcgaagagg gagcgcgcgg cgtcgaacgg
     8521 gggaggggcg gcggcgcaga ggccgcaggg ggacggatga ggaccgggtc cggaaccttc
     8581 gaatgcgccg caccgggggc acacagcttc ggtgtgggcg gaccaccggg cgatggcgga
     8641 gggcgaaagg tcgggggacg cggccggcag gaggtcacgg agcgacgcga gttcaccgat
     8701 ccattgcgcc aacgcgaagc gcgcccgctt ccgacggcgg caaggttgcg cggcgacggg
     8761 cggaggttca gcggagggtg gcggagacgg cgtactcccg gtttccgtgg tcgatggcgt
     8821 acccgttcca tatcccgcag gaggggcacc gtgcggtcca cttgatggtg gaggaatcgc
     8881 aggaggaaca ccggaagggg atgaggtatc tccgcttgta accgaaggcc cgctggtagt
     8941 gcagactcgc cgattcatac cgcccccttc gccggaacgc ttcagcgagc aggatgttca
     9001 ccgactcgcg ctccgggccc agggtttcgg ctttcaacaa ctgttccagt ccctcgtcga
     9061 tcatctcaag ccgcaggaaa aactttccca tgaagaggtt gacgtcgtag tcggacggga
     9121 attcc
//
LOCUS       DASVJL010000193         5162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_310101, whole genome shotgun sequence.
ACCESSION   DASVJL010000193 DASVJL010000000
VERSION     DASVJL010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310101"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2278
                     /locus_tag="VF357_03980"
     CDS             <1..2278
                     /locus_tag="VF357_03980"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEX7523338.1"
                     /translation="HTGDGRIEKIVNNYARISFNFGPTLLSWMEEKSPAVYRAILEGD
                     QESRKTFSGHGSALAQGYNHIILPLANARDKDTQVRWGIRDFTHRFGRPPEGMWLPET
                     AVDLETLEVLAREGIRFTILAPHQGAQVREKGAEDWNDVSGGRIDPTIPYELDLPSGR
                     KISIFFYDGPISQGVAFERLLDNGEFLANRLLGAFSDQRPWPQQLVHIATDGETYGHH
                     HAHGEMALTHAMRLFDANPEIRVTNYGEHLEKFPPTHEVRILDDSSWSCVHGVERWRS
                     DCGCNSGGRPGWNQEWRGPLRDALDWLRDTIAPLFEEEGRKVFKDPWAAREEYISVIL
                     DRSDASVDEFLGRHAAGELTSEQKTRALELMEIQRHAMLMYTSCGWFFDELSGIETVQ
                     VLQYAGRVVQLSGEVFGDAVETGFLERLERAKSNVPEQKDGRALYDKFVKPATVDLHK
                     VAAHYAVSALFENFGERSRIFCYDVEREDFRVQTAGRTKLLLGKARVTSEITRKSAFM
                     SFGLLHLGDHNVSGGIRDFRDEEAYETLTREIGDVFKTADLPEVIRTVDKHFGLETYS
                     LKLLFRDELRKILHIILDQTLSETEGSHRQIYQQHATLMQFLSSLSIPLPDPLYASAK
                     VALNTDLRRAVSGESLDASVINGLLEEGRSIGIPVDSAGLGFALQKRVEAIADDFREK
                     PDDLDLLRRFEEAVGTANALPIEVLLWGAQNTYDELLKTAYPGFLAKSVNGDEQAALW
                     VGRFRSVGKQLSFLVPKD"
     gene            2282..5161
                     /gene="treY"
                     /locus_tag="VF357_03985"
     CDS             2282..5161
                     /gene="treY"
                     /locus_tag="VF357_03985"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX7523339.1"
                     /translation="MTALRIPAATYRLQFHRRFRVEHATGLAPYLDALGITDLYASPI
                     FQARRGSSHGYDVTDPTRLNVELGTEENFESLVSALKERGMGLLLDIVPNHMAASVEN
                     RWWTDVLESGQGSPFSGFFDIDWHSPKKALTNKVLLPILGGPYGRMIEEQEISLRLEK
                     GGFTVHCSSVKLPVSILSYPRILSLRLPSLEETYGPDHPSFRELWDLITTIEHLPKAA
                     EEDAEAARERHASEEGIKERLLRLYTERHEIREHIDETLRMVNGRKGDPASFDPLDRI
                     LSEQHYWLSFWRLANEEINYRRFFAVSELVSLRVEDPRVFDASHDLILRLAREGKITG
                     LRVDHVDGLYDPHGYLVRLMQRLTGSEDENPSPGFYVLVEKILGRDEALTPAWPIHGT
                     TGYDFMNALNGVFLNAGGVKTLDEIYARFTGTTPDFRETVYRSKRLMMDTLFAGEMHS
                     LGQHLGRLAEQDRYARDLPRKELRAAVIEVTACFPVYRTYARSPELSARDVRYIAKAL
                     KEAQQRSTEASTPVFDFLRRVLLQEIPPSLAGNDREDWLRFLMRWQQFTGPIMAKGYE
                     DTSLYIYNRLVSMNEVGGDPSSTGISVSAFHDRGKIMASRWPHSMNATSTHDTKRSED
                     VRARINVLSEIPEEWEKRLLQWSRQNAGKKRIVNGIATPDPGEEILLYQTLLGVWPLA
                     EEELPGLKSRVESYMVKAMREAKVYTRWIRPNIAHEGAVKEFVASLLGDLSPDTNLFL
                     ADFLPFQRKVAHYGAINGLAQLLLKIASPGVPDIYQGTEIWDFSLVDPDNRRPVDYPH
                     RTGLLNELMTLERQGLSSLARDLLSSWGDGRVKLFVTYKALSFRRERKELFQDGAYVP
                     LAVTGGVKEHALAFARKRGDAWGIAAVPRLVTRLAPPGEFPIGLGVWEARAAIRLPKE
                     APDGWRNVFTGEILHASGGDGAKLLHLHAVFHDFPVALLEGLPEP"
BASE COUNT          954 a         1705 c         1671 g          832 t
ORIGIN      
        1 ccacaccggg gacgggcgga tcgagaagat cgtcaacaac tacgcccgca tcagcttcaa
       61 cttcggtccg acgctgctgt cctggatgga ggagaaatcc cccgcggtgt accgcgccat
      121 cctcgaaggg gaccaggaaa gccgcaaaac gttctccgga cacggctccg ccctggcgca
      181 agggtacaac cacatcatcc ttccccttgc caacgcgcgg gacaaggata cgcaggtccg
      241 ctgggggatc cgggatttca cccaccgctt cggccggcct ccggagggga tgtggctccc
      301 ggagaccgcc gtggaccttg aaaccctcga ggtcctggcc cgggagggga tccggttcac
      361 gatcctcgcc ccccaccagg gggcccaggt gcgggagaag ggggccgaag actggaacga
      421 cgtcagcggc ggccggatcg atcccacgat cccgtacgag ctcgatcttc cctcggggcg
      481 gaagatctcc atcttcttct acgacggacc catctcccag ggggtcgcct tcgagcggct
      541 tctcgacaac ggggagttcc tcgccaaccg cctgctgggg gcattctccg accagcgccc
      601 gtggccgcag cagttggtgc atatcgccac cgacggcgag acgtacggcc accaccacgc
      661 ccacggagag atggcgctta cgcatgcgat gcgccttttc gacgcgaacc cggagatccg
      721 cgtcacaaac tacggagagc acctggagaa attcccgccc acccacgaag tgcggatcct
      781 cgacgacagt tcctggagct gcgtccacgg cgtggagcgg tggcgaagcg actgcggatg
      841 caactccggc ggccgccctg ggtggaacca ggagtggcgg ggaccgctgc gggatgcgct
      901 ggactggctc cgggacacca tcgcccccct gttcgaagag gaagggagaa aggtcttcaa
      961 ggacccttgg gccgcccggg aagagtacat ctcggtgatc ctcgaccggt ccgacgcctc
     1021 cgtcgacgaa tttctcggaa ggcacgccgc cggggagctg acgtcggagc agaagacccg
     1081 ggcgctcgag ctgatggaga tccagcggca cgccatgctc atgtacacga gctgcgggtg
     1141 gttcttcgac gagctctcgg ggatcgaaac ggtgcaggta ctccagtacg ccggccgcgt
     1201 cgtacagctt tccggggagg tcttcggcga cgccgtcgaa accggtttcc tggagcggct
     1261 cgagcgggca aaaagcaacg tgccggaaca aaaggacgga cgcgccctgt acgacaagtt
     1321 cgtaaagccg gccacggtgg acctgcacaa ggtcgcggcc cactacgcgg tgagcgccct
     1381 cttcgagaat ttcggcgaac gctcccggat cttctgctac gacgtcgagc gcgaggactt
     1441 ccgcgtccag acggcgggca ggacgaagct cctcctgggg aaggcgcggg tcacctcgga
     1501 gatcacgcgg aaatcggcat tcatgtcgtt cggcctcctg cacctggggg atcacaacgt
     1561 gagcggaggg atccgcgact tccgggacga ggaggcgtac gagaccctca cccgggagat
     1621 cggcgacgtc ttcaagaccg ccgacctccc ggaggtgatc cggaccgtgg acaagcattt
     1681 cgggctggaa acctattcgc tgaaactgct gttccgggac gagctgcgga agattctgca
     1741 cataatcctc gaccagaccc tctccgagac ggaagggagc caccggcaga tctaccagca
     1801 gcacgccact ctgatgcagt tcctttcgag cctgagcatc ccccttcccg atcccctgta
     1861 cgcctccgcg aaggtcgcgc tcaacacgga tctgcgccgt gccgtttcgg gagagagcct
     1921 cgacgcgtcc gtcatcaacg ggctcctgga agagggccgc agcatcggca tcccggtcga
     1981 ttcggcgggc ctcgggttcg ccctgcagaa gcgggtcgag gcgatcgcgg acgacttccg
     2041 ggagaaaccc gacgatctcg acctgctccg ccgattcgaa gaggcggtgg gaacagccaa
     2101 cgcgctcccc atcgaagtgc tcctctgggg cgcccagaac acctacgacg aactgctgaa
     2161 gaccgcctac cccggtttcc tggcgaagtc cgtcaacggc gacgaacagg cggctctctg
     2221 ggtcggtcgt ttccgttccg tcggaaagca actttctttc ctcgtcccaa aggattgacc
     2281 cgtgacggcg ctccggattc cggcagccac ctaccggctc cagttccacc ggcggttccg
     2341 ggtcgaacac gccaccggcc tggcgccgta cctggacgct ctgggcatca ccgacctcta
     2401 cgcttcgccg atcttccagg cgcgccgggg aagttcgcac gggtacgacg tgaccgaccc
     2461 cacccggctc aacgtcgagc tggggaccga ggagaatttc gaatcgctcg tgagcgcgct
     2521 gaaggagcgc ggcatggggc tgctgctgga catcgtcccc aatcacatgg ccgccagcgt
     2581 cgagaaccga tggtggacgg acgtcctgga gagcggacag gggtcgccgt tctcgggatt
     2641 cttcgacatc gactggcact cccccaagaa ggcgctgacc aacaaggtcc ttctccccat
     2701 cctcggcgga ccgtacggcc ggatgatcga ggagcaggag atctccctgc ggctggaaaa
     2761 gggcggcttc accgttcact gctcctccgt gaagcttccg gtgtccatcc tgtcgtaccc
     2821 gcggatcctt tcccttcggc ttccgtcgct cgaggagacc tacgggccgg accacccgtc
     2881 gttccgcgag ctctgggacc tgatcacgac gatcgaacac ctcccgaagg ccgccgagga
     2941 ggatgcggag gcggcccggg agcggcacgc gtccgaagag gggatcaagg agcggctcct
     3001 gcgtctgtac acggaacgcc acgagatccg ggagcacatc gacgaaactt tgcggatggt
     3061 gaacgggcgg aaaggcgatc ccgccagctt cgatcccctc gaccgcatcc tctccgagca
     3121 gcactactgg ctgtccttct ggcggctggc caacgaggag atcaactacc ggcggttctt
     3181 cgcggtcagc gaactggtca gcctccgggt cgaggacccc cgggtcttcg acgcctccca
     3241 cgacctgatc ctgcggctgg cgcgggaggg gaagatcacc ggcctgcgcg tggaccacgt
     3301 cgacggcctt tacgatccgc acgggtatct cgtccggctc atgcagcggc tgacggggtc
     3361 ggaggacgaa aacccttccc cgggattcta cgtcctggtc gaaaagatcc tcgggaggga
     3421 cgaggcgctt acgcccgcct ggccgatcca tggaacgacg ggatacgact tcatgaacgc
     3481 gctgaacggg gtcttcctca acgccggggg cgtcaagacc ctggacgaga tctacgcgcg
     3541 gttcaccggc accacgccgg atttccggga gacggtgtac cggagcaaga gactgatgat
     3601 ggacaccttg ttcgcggggg aaatgcactc gctgggccag cacctcgggc ggctggcgga
     3661 gcaggaccgg tacgcgcggg accttccccg gaaagagctt cgcgccgcgg tgatcgaggt
     3721 cacggcctgc ttccccgtct accggacgta cgcccggagt cccgagctgt ccgcccggga
     3781 cgtccggtat atagcgaaag ccctgaaaga ggcgcagcag cgcagcaccg aggccagcac
     3841 ccccgtgttc gacttcctga ggcgggtcct gctgcaggag atccccccgt ccctcgccgg
     3901 gaacgaccgg gaggattggc ttcgtttcct gatgcgctgg cagcagttca cgggcccgat
     3961 catggcgaag gggtacgagg acacctccct gtacatctac aaccggctcg tctcgatgaa
     4021 cgaggtcggc ggcgacccct cctccaccgg gatctccgta agcgcgttcc acgaccgcgg
     4081 caaaatcatg gcctcccgct ggccccattc catgaacgcc acgtcgaccc acgacaccaa
     4141 gagaagcgag gatgtccggg cgcggatcaa cgtcctctcg gagatcccgg aggaatggga
     4201 gaagcgtctc ctccagtgga gccggcagaa cgccggaaag aaacggatcg tcaacggaat
     4261 cgcgacgccg gacccgggcg aggagatcct cctctaccag acgcttctgg gagtgtggcc
     4321 ccttgcggag gaggaactcc cggggctgaa atcccgggtc gagagctaca tggtgaaagc
     4381 gatgcgggag gccaaggtct acacacgatg gatacgaccc aacatcgccc atgaaggcgc
     4441 ggtgaaggag ttcgtcgcct ccctgctggg ggatctctcc cccgacacca acttgttcct
     4501 ggccgatttc ctccccttcc agcgaaaggt cgcccattac ggtgcgatca acggcctggc
     4561 ccagctcctg ttgaagatcg cctctccggg agttccggac atctaccagg ggacggaaat
     4621 ctgggacttc tcgctggtgg acccggacaa ccgccggccg gtggattacc cgcaccgtac
     4681 cggcctgctc aacgagttga tgaccctgga gcgccagggg ctctcctccc tcgcccggga
     4741 tctcctctcc tcctgggggg acgggcgggt aaagcttttc gtgacgtaca aggcgctctc
     4801 tttccggcgg gagcggaagg agctgttcca ggacggcgcc tacgtgcccc tggccgtgac
     4861 gggcggggtg aaggagcacg cgctggcgtt cgcgcgaaaa cggggcgatg catgggggat
     4921 cgcggcggtc ccgcgcctcg tgacccgcct ggcgccgccg ggggaattcc ccatcggcct
     4981 cggggtctgg gaagcgcgag ccgccatccg gctgccgaag gaggctccgg acggatggcg
     5041 taacgttttc acgggggaga tccttcacgc ttccgggggg gacggagcga agctgcttca
     5101 cctccacgcg gtgttccacg acttcccggt ggcgcttctt gagggcctcc cggagccgtg
     5161 aa
//
LOCUS       DASVJL010000194         3192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_874003, whole genome shotgun sequence.
ACCESSION   DASVJL010000194 DASVJL010000000
VERSION     DASVJL010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874003"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VF357_03990"
     CDS             <1..234
                     /locus_tag="VF357_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX7523340.1"
                     /translation="GLHRAIMLRAISIISRSGGVTNEFTFTGGVAKNEAAVRELGKLV
                     KENYGDMTINISPDSIYTGALGGANFALRAVVN"
     gene            complement(342..1253)
                     /locus_tag="VF357_03995"
     CDS             complement(342..1253)
                     /locus_tag="VF357_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX7523341.1"
                     /translation="MKAMILAAGLGTRLRPLSLEIPKPVIPVLGQPLCGHAMSFLREH
                     GAESFLLNLHYGPETVRKKVTAWAAGRFPVEFTHEPEILGTGGGIGNAREYLRGETFV
                     TANSDAVARFPLGEAFARHRANGALATLVLFPDRWKRYTPVCVREDGRIAGFGGVAPA
                     GGFEGFYTGYLIAEPEMLDPIPRGRPSCIVRDTLVPLIATGAPICAFMTEGTFLDFGT
                     PEDYLRGTLALLAKRGGDKGPHSFAHPRASIGEGAIVGPDAVIEEGASVGAGATVRRA
                     ILWPGAVVPPGALVENGILTPRGFVPA"
     gene            complement(1250..2338)
                     /locus_tag="VF357_04000"
     CDS             complement(1250..2338)
                     /locus_tag="VF357_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7523342.1"
                     /translation="MTEGIDLAALRAGLAALFPATDPMSAKVSELAGDASTRRYYRVR
                     NAPGATLPSVVVMRYPDDLSPETELPFLNVHRYLTAAGVPVPVVYRFDPKANLLFLED
                     AGDTMLEDAVRDHGVRGCLPLYEQCVEILVRIQSEGTSALDGKAIPRGLSFDVAKFTG
                     EIDFFFLHAVREYGGIRLSDREERAIVDLILPFLEQLSAFPRVLAHRDYHSRNVMVLG
                     SAKTPGHRNLRVLDFQDARMGNVFYDLASLLRDSYVTLPENAVENLRYAWRNAATAEL
                     RGAAGDPGAFAWRFDLAALQRNVKAIGTFGNQAHNRGKRIYLRFILPTVVHLRRNFER
                     NPPMRALAGKLLPILSALAEKAAAESPS"
     gene            complement(2335..>3192)
                     /locus_tag="VF357_04005"
     CDS             complement(2335..>3192)
                     /locus_tag="VF357_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX7523343.1"
                     /translation="RKDSLNVSLSTHLFAFHDLDEAIFPLFPRYGFSLAEIWAMPPHF
                     PSGDFPAADGVARRMAEHGIRVASVHAPLYPDIRTYKKDRWYSLSSVDEAHRLESVAV
                     TARTAGWLASNGGGTVVLHTSFPAGQWYPHRWGAFLSSMNELLDAVPAGVRFAVENTP
                     VDSGQVSVILDIVDRYPADRVGVCLDIGHAHIEENALSAVCAAGPRLIHVHASDNHGE
                     KDDHLVPGKGEIPWNGVTKALRETGFDGSFTVELRDYTRGENPAYKDFDQILSECRTS
                     LDRIFRDPP"
BASE COUNT          538 a         1045 c         1124 g          485 t
ORIGIN      
        1 gggctgcacc gggcgatcat gctgcgcgcg atctccatca tctcgcgctc cggcggggtc
       61 acgaacgagt tcaccttcac cgggggggtc gcgaagaatg aagcggccgt ccgcgagctg
      121 ggcaagctgg tgaaggagaa ctacggcgac atgaccatca acatcagccc cgattcgatc
      181 tacacggggg ccctcggggg cgcgaacttc gccctgcggg ccgtcgtcaa ctagcggagt
      241 cgccgcagga gggggggcga agtgaggtaa agcgcagccg tgcaggttca tcgcgcggcg
      301 agccacgaac ggagccccgc cctccgaggc gacgcagccg actaagcggg gacgaacccg
      361 cgcggcgtga ggatcccgtt ttcgacgagc gccccgggcg gcacgacggc gccgggccag
      421 aggatcgcgc ggcgaacggt ggcgccggcc cccacggagg cgccctcctc gatgaccgcg
      481 tcgggtccga cgatcgcgcc ttctccgatc gatgcccggg ggtgcgcgaa ggagtgcggt
      541 cccttgtcgc ctccgcgctt cgcgaggagg gcgagcgttc cgcgcaggta atcttccggc
      601 gtcccgaagt cgaggaaagt cccctccgtc atgaacgcgc agatcggcgc ccccgtcgcg
      661 atcagtggga ccagggtgtc ccggacgatg cacgacggcc ggccgcgcgg aatcgggtcg
      721 agcatctcag gctccgcgat caggtaaccg gtgtagaacc cctcgaaccc cccggcgggg
      781 gcgacgccgc cgaaccccgc gatccgcccg tcctcccgca cgcacacggg ggtgtaccgc
      841 ttccatcggt ccgggaagag gacgagggtg gccagcgccc cgttcgcacg atggcgcgcg
      901 aaggcctccc cgagcgggaa ccgcgcgacc gcgtcggaat tggcggtcac gaacgtctct
      961 ccgcgcaggt actccctcgc gtttccgatc ccgccgccgg tcccgaggat ctccggctcg
     1021 tgcgtgaact ccaccgggaa ccggcctgcc gcccacgccg tcaccttctt ccggaccgtc
     1081 tcggggccgt agtgcagatt gaggaggaac gactccgcgc cgtgctcgcg gaggaacgac
     1141 atcgcgtggc cgcacagcgg ttggcccagc acgggtatga caggtttcgg gatctcgagg
     1201 gagaggggac gcaggcgcgt tccaagcccc gcggcgagga tcatcgcctt catgacgggc
     1261 tctccgccgc cgccttttcg gccagcgccg acaagatcgg gaggagcttt ccggcgaggg
     1321 cgcgcatcgg cgggttccgt tcgaagtttc gccgcaggtg gacgaccgtg ggcaggatga
     1381 agcgaaggta gatccgcttc cctcggttgt gcgcctggtt gccgaaggtc cctatcgcct
     1441 tcacgttgcg ctgaagcgcg gccagatcga acctccaggc gaaggcgccg gggtcccccg
     1501 ctgcgccgcg cagctccgcc gtggcggcgt tccgccaggc gtagcgaaga ttctcgaccg
     1561 cgttctccgg caaggtgacg taggaatcgc gcaggagcga ggccaggtcg tagaagacgt
     1621 tccccatccg ggcgtcctgg aagtcgagga cccgcaggtt ccggtggccg ggagtcttcg
     1681 cagatccgag caccatcacg ttgcggctgt ggtaatcccg gtgagcgagc acacgcggga
     1741 aggcggacaa ctgttcgagg aacggaagga tgagatccac gatcgcccgc tcttcccggt
     1801 cggacaaccg gatcccgccg tattcccgca cggcgtgcag gaagaagaag tcgatctccc
     1861 cggtgaattt cgcgacgtcg aaggagaggc cccgtgggat cgccttgccg tcgagcgcgc
     1921 tggtaccctc ggactggatc cggaccagga tctcgacgca ctgctcgtag agcggcaggc
     1981 agccgcgcac cccatggtcg cggaccgcgt cctccagcat cgtatccccc gcgtcttcga
     2041 ggaagagaag gttcgctttc gggtcgaacc ggtacacgac cgggaccggg actccggcgg
     2101 cggtcaggta ccggtggacg ttcaggaagg gtagctccgt ctccggcgat aaatcgtccg
     2161 ggtaccgcat cacgacgacg gaggggagcg tggcgccggg ggcgttccgg acgcggtagt
     2221 aacgccgtgt ggaggcgtcc cccgcgagtt cggacacctt ggccgacatc ggatccgttg
     2281 cgggaaacag ggcggctagg ccggcgcgca gcgccgcgag gtcgattcct tcggtcaagg
     2341 gggatcccgg aagatccggt cgagagaggt gcggcactcg gaaagaatct ggtcgaagtc
     2401 cttgtatgcc gggttctccc cgcgggtgta atcccgaagt tccacggtga aggagccatc
     2461 gaagccggtt tcccgcaacg ccttcgtcac gccgttccag gggatctctc cttttcccgg
     2521 gacgagatgg tcgtcctttt ccccatggtt gtccgaggcg tggacgtgga tcaggcgggg
     2581 gcccgctgcg cacacggcgg aaagcgcgtt ctcctcgatg tgcgcatgtc cgatgtccag
     2641 gcagacgccc acccggtccg ctggataccg gtctacgatg tcgaggatga cgctcacctg
     2701 gccggagtcg accggggtgt tctcgaccgc gaaccggacg ccggcgggga ccgcgtcgag
     2761 tagttcgttc atcgaggaga gaaaggcccc ccaacggtgc gggtaccatt gtccggccgg
     2821 aaacgaggtg tgcaggacca ccgttccccc gccgttgctc gcgagccacc cggcggttcg
     2881 ggcggtcacg gcgacggact cgagccggtg cgcctcgtcc acggaggaga gggagtacca
     2941 ccggtccttc ttgtacgtcc gaatgtccgg atacaggggg gcgtgaacgc tcgccacccg
     3001 gatgccgtgc tccgccatcc gccgcgcgac cccgtcggcc gccggaaagt cgccggacgg
     3061 gaagtggggg ggcatcgccc agatctccgc gagggagaag ccgtaccggg gaaagagcgg
     3121 aaaaatcgcc tcgtccaggt catggaaggc gaacaggtgc gtcgacaggg aaacgttcaa
     3181 ggagtccttc ct
//
LOCUS       DASVJL010000195         1807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_591561, whole genome shotgun sequence.
ACCESSION   DASVJL010000195 DASVJL010000000
VERSION     DASVJL010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591561"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VF357_04010"
     CDS             <1..492
                     /locus_tag="VF357_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7523344.1"
                     /translation="AEKGNAEAFAGYGRALLYGRGVAQNESAAADWIRKAAERGVPEG
                     IFLRGVLHAYGRGEPKDGAEALDEYRRAAEEGYVDGQLQIAGMYLTGRGAKQSDNTAA
                     AWYLRAADAGSPDAQVMLGWMHEVGRGVPKDLGESVRWFRLAAAQGNTVARDHLKYLE
                     TLH"
     gene            complement(497..>1807)
                     /locus_tag="VF357_04015"
     CDS             complement(497..>1807)
                     /locus_tag="VF357_04015"
                     /inference="COORDINATES: protein motif:HMM:NF044721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523345.1"
                     /translation="VILDVVYNHTGEGNELGPTFSFKGIDNPSYYVLTGGPHDPARYY
                     MNWTGCGNSFNLSTPQTIRLVMDSLRYWVETMHVDGFRFDLASVLGREGGMYQRCASF
                     FDAVSQDPVLQKTKLIAEPWDLGSYEVGNFPVDWSEWNGRFRDTVRRFVKGDGGQLRD
                     LGFRLTGSADLYADDGRSAYNSVNFVTCHDGFTLRDLVSFDGKHNEANLEGGRDGTDD
                     NNSWNCGAEGETDDPEIVRLRRRLARNHACLLLFSSGTPMILGGDEFLRTQGGNNNAY
                     CQDNPISWFDWGEVERNADMVAFFRKAIAFTKKYTILQRRKFFTGTDRDGNAVPDIQW
                     IGEDLGPPRWDDPEARVLCYLLDGSEEPSEAGPYFLFLALNGEFRQREVMVPPLPSGL
                     RWRRAIDTSLAAGEDFVDDGAELLPDPADRYLVNPRTTVVLLGK"
BASE COUNT          292 a          600 c          617 g          298 t
ORIGIN      
        1 gcggagaagg ggaacgcgga ggcgttcgcg gggtacggac gggcgcttct ctacggccgc
       61 ggcgtggccc agaacgagtc ggcggcggcg gactggatcc ggaaggccgc ggagcgcggc
      121 gtcccggaag ggatcttcct ccggggtgtc ctccacgcct acgggcgggg cgagccgaag
      181 gacggagcgg aggcgctcga cgagtaccgg cgcgcggcgg aggaggggta cgtcgacgga
      241 cagctgcaga tcgccgggat gtacctcacg gggcgcggag cgaagcaaag cgacaacacg
      301 gccgccgcct ggtacctgcg cgcggcggat gcggggagtc cggacgccca ggtgatgctc
      361 gggtggatgc acgaggtcgg ccggggcgtt ccgaaggacc ttggcgagtc ggtccgctgg
      421 ttccgcctcg cggcggccca ggggaacacc gtcgcccgcg accatctgaa gtacctcgag
      481 acccttcact gactgtttat ttccccagca gtaccaccgt ggttcttgga ttgacgagat
      541 atcggtcggc cggatccggc aggagttccg cgccgtcgtc cacgaagtct tctcccgccg
      601 cgaggctcgt gtcgatcgcg cgccgccatc ggagtcccga tgggagcggc ggcaccatca
      661 cctctcgctg ccggaactcc ccgttcaggg cgaggaagag gaagtacggc cccgcctccg
      721 acggctcctc gctcccgtcg aggagatagc acaggacccg cgcttccgga tcgtcccagc
      781 ggggaggccc gaggtcctcg ccgatccact ggatgtcggg gactgcgttc ccgtcccggt
      841 cggtgccggt gaagaacttg cgccgctgga ggatcgtgta tttcttcgtg aacgcgatcg
      901 ccttccggaa gaaggcgacc atgtctgcgt tccgctcgac ctccccccag tcgaaccagg
      961 agatcgggtt gtcctggcaa taggcgttgt tgttcccccc ctgcgtccgg aggaattcgt
     1021 cgcccccgag gatcatcggg gtcccggagg agaagagcag caggcaggcg tggttcctcg
     1081 ccagccgccg ccgcagccgc acaatctccg ggtcgtccgt ctccccttcc gcgccgcagt
     1141 tccaggagtt gttgtcgtcc gtgccgtccc ggcctccctc gaggttcgcc tcgttgtgct
     1201 ttccgtcgaa ggagaccagg tcgcgcaggg tgaacccgtc gtggcaggtg acgaagttga
     1261 cgctgttgta ggcggaacgt ccgtcgtcgg cgtacagatc ggccgacccc gtcagccgga
     1321 atccgaggtc ccgaagctgc ccgccgtccc ccttcacgaa acggcgcacc gtgtcgcgga
     1381 accggccgtt ccactccgac cagtccacgg ggaagttccc cacctcgtac gacccaaggt
     1441 cccacggttc ggcgatgagc ttggtcttct gcaacaccgg gtcctgggag acggcgtcga
     1501 agaaggaggc gcaccgctgg tacatcccgc cctcgcgccc cagcacggag gcgagatcga
     1561 accggaaccc gtcgacgtgc atcgtttcga cccagtaccg caacgaatcc atcaccagcc
     1621 ggatcgtctg gggggtcgac aggttgaagg agttgccgca acccgtccag ttcatgtagt
     1681 agcgcgccgg gtcgtggggc cccccggtca gcacgtaata cgacggattg tcgatcccct
     1741 tgaaggagaa ggtggggccc agctcgtttc cctcccccgt gtggttgtac accacgtcga
     1801 ggatcac
//
LOCUS       DASVJL010000196         1719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1155313, whole genome shotgun sequence.
ACCESSION   DASVJL010000196 DASVJL010000000
VERSION     DASVJL010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155313"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VF357_04020"
     CDS             complement(<1..577)
                     /locus_tag="VF357_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523346.1"
                     /translation="MKTTLRKKDLDLLVGARHWDPFSVLGPHVVEEGGKRFVSVRVIL
                     PRAREVQVMRDTGATRRASMIRIHPDGVFEAHFPLDTEIFPYRLEITVGDGYRWTQHD
                     PYAFGTVLTDFDIHLLSEGSHLAQYEKLGSHVVDIGGIRGTTFSVWAPNAERVSVVCN
                     FNHWDGRVHPMRNRGESGIWEIFLPGVVEEEV"
     gene            complement(645..1265)
                     /locus_tag="VF357_04025"
     CDS             complement(645..1265)
                     /locus_tag="VF357_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HEX7523347.1"
                     /translation="MRKIVSLAAIAALFMSILSGCAAQNEGLREHQGAAVGAGTGAVG
                     GAVVGGLLGGRRGAVAGGLLGALAGGLLGNYHDEREKNLADTRRDYTEYNAARGTRLK
                     IERVRTNPPEAVPGDTVEIQLTYAVLTPREDVLVPVRENREILFNGSKVGEASVDIER
                     EGGTWRSVVPITLPPNARPGNYRVVASVESRGGGKDIEEITFRVHR"
     gene            complement(1338..>1719)
                     /locus_tag="VF357_04030"
     CDS             complement(1338..>1719)
                     /locus_tag="VF357_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523348.1"
                     /translation="DVRVSMATDLGQNENGLFERFLDSDLILIEANYDEEMLNRSPRL
                     DRGRVGSNVGHFSNAQAGKFLVRVLQESRKAPQAVVLCHLSADHNTPELARRTVREIL
                     SGHRFGDVPVHAARRGGPTRKFTV"
BASE COUNT          261 a          598 c          565 g          295 t
ORIGIN      
        1 agacctcctc ctcgacgacg ccggggagga agatctccca gatcccggac tctccccggt
       61 tgcgcatcgg gtgcacgcgg ccgtcccaat ggttgaaatt gcagacgacg gagacgcgct
      121 ccgcgttggg cgcccacacc gagaacgtcg ttccccggat cccgccgatg tcgacgacat
      181 ggctccccag cttctcgtac tgcgccaggt ggctcccttc ggagaggaga tggatgtcga
      241 agtcggtgag gaccgttccg aacgcgtagg ggtcgtgctg cgtccaccgg tagccgtcgc
      301 cgacggtgat ctcgagacgg tacgggaaga tctccgtgtc gagcgggaag tgcgcctcga
      361 acaccccgtc ggggtggatg cggatcatgg aggcgcgccg cgttgcgcca gtgtcgcgca
      421 tcacctggac ctcgcgggcc cgcggcagga tcacccgcac ggaaacgaaa cgcttccctc
      481 cttcctccac gacatggggg ccgaggacgg agaaggggtc ccagtgacgg gcgcccacga
      541 ggaggtcgag gtccttcttc cggagggtgg tcttcatcgc acctcaattg tagcggaaac
      601 ggaaggagaa ggggccgccg cggtggccgg ccccgttgaa tgcatcaccg gtgtacgcgg
      661 aacgtgatct cctcgatgtc cttccctcct ccgcgcgact ccaccgaggc gacgacacgg
      721 tagttccccg gacgggcgtt cgggggtagc gtgatgggga cgacggagcg ccaggtgcct
      781 ccctcgcgct cgatgtcgac cgaggcctcc cccaccttgc tgccgttgaa gaggatctcg
      841 cggttctccc gtacggggac caacacgtct tcccgcgggg tcaggacggc gtaggtcaac
      901 tggatctcga ccgtgtcgcc cgggacggcc tcgggggggt tggtccgcac ccgctcgatc
      961 ttgaggcgcg ttcccctggc cgcgttgtac tccgtgtagt cgcgccgcgt atccgcgaga
     1021 tttttctccc gctcgtcgtg gtagttgccg agcagcccgc cggcaagcgc gccgagcagc
     1081 ccgccggcga ccgcgccgcg ccttccgccg agcagccctc cgaccaccgc tccgcccacc
     1141 gccccggtcc cggcgccgac cgccgccccc tgatgctccc gcaacccctc gttttgcgcg
     1201 gcgcatccgg acaggatcga catgaacagg gcggcgatcg ccgcgaggct cacgattttg
     1261 cgcataaaca ccctcccgcg atttctcgcc actttttgac gcatttggcg ctcgccgtat
     1321 tcaaaggcaa accggcgtca gacggtgaac ttccgcgttg gtcctcccct gcgggccgcg
     1381 tggacgggaa cgtcgccgaa gcggtggccg gagaggatct cccgaacggt gcggcgggcc
     1441 agttccgggg tgttgtgatc cgcgctcaag tggcacagga cgacggcctg cggcgccttc
     1501 cggctctcct ggaggacccg caccaggaat ttcccggcct gcgcgttcga gaagtgcccc
     1561 acgttcgacc ccacccgtcc ccggtcgagc cgggggctcc ggttcagcat ctcttcgtcg
     1621 tagttcgcct cgatcaggat caggtcgctg tcgaggaacc gctcgaagag gccgttctcg
     1681 ttctggccga ggtcggtcgc catcgacacg cggacgtcc
//
LOCUS       DASVJL010000197         1519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1436903, whole genome shotgun sequence.
ACCESSION   DASVJL010000197 DASVJL010000000
VERSION     DASVJL010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436903"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1346
                     /locus_tag="VF357_04035"
     CDS             255..1346
                     /locus_tag="VF357_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7523349.1"
                     /translation="MDRRSVAAILGVILWSCVGGVSAGAGTSTLLSPASARESSLLSA
                     ILRHSATGSRDLLGLPVDPSLPEREISRLADRLRPEVVGAIDGGQIVSAFRRVLLVEE
                     RFTYDRSSADPGNYLLESVIARRKGNCLGLSLLFLSLADRLGIPFHGVYAPSHCFVRY
                     EGNGVRVNVEFAENGASWEDDRYRRVFGISPGRPYLYSLSPTEMLGVFLKTLGAGYSR
                     KGREEEALRLYDEAERLYPESPDVHYNAGVSLQKLGRPDEAAGKYRRALALDPEMAAA
                     RDNLSILLAGMGRYDDAIAEARRAVELEPWNAATRGNLAAAYCGCGRFDEGIREYRTA
                     VEIDPGNRRLQAGLKRAYISRGSHREVAP"
BASE COUNT          237 a          468 c          533 g          281 t
ORIGIN      
        1 gacggaggaa aacggggggt ggctgaatat ggacgtggaa gtacagaatt cgcacccgga
       61 gctttggggc ctccccaaca tccagagcgg cggcggcagc tagtagcgta gcaggacgtg
      121 cttgtcgcaa gggaacaacg acgccgggaa gaatctttcc cggcgtcgtt tcctttggtg
      181 gtgcgtccga cgggggcaca aggtaagatg gaaaggaggt aggtcccggg gggtgccccg
      241 ttggaggcag gtgggtggat cgtcgatccg tcgcagcaat ccttggggtg atcttgtggt
      301 cgtgtgtcgg cggcgtgtcc gccggcgccg ggacttccac ccttctttcg cccgcctccg
      361 cgagagagtc ttccttatta tctgccatcc ttcgccattc cgccacgggg agccgcgacc
      421 ttctcggtct cccggtcgat ccgtcgctgc ctgaacggga aataagccga ctcgcggatc
      481 gcctgcgccc cgaggtggtc ggtgccatcg acgggggtca gatcgtcagc gccttccgtc
      541 gcgtccttct cgtcgaggaa cgattcacct acgacaggtc gtccgcggac ccggggaatt
      601 atcttctcga atccgtcatc gcccgcagaa aagggaactg cctcggactg tcgttgctct
      661 tcctttctct tgcggatcgg ctcggcatcc ctttccatgg ggtgtacgcc ccgtcccatt
      721 gtttcgtccg gtacgagggg aacggcgtcc gcgttaacgt ggagttcgcg gaaaatggcg
      781 cttcttggga ggatgaccgg taccggcggg tgttcgggat ctcacccggg aggccctatc
      841 tctattctct ttcccccacc gagatgctcg gcgtgttcct gaagacgttg ggggcggggt
      901 actcccggaa ggggcgggag gaggaagcgc tccggctgta cgacgaggcg gagcgcctct
      961 acccggagtc gcccgacgtg cactataacg ccggggtctc cctgcagaaa ttggggcgtc
     1021 ccgacgaggc ggccgggaag tatcgccggg cattggccct tgacccggag atggcggccg
     1081 cccgggacaa cctgagcatt ctcctggccg ggatggggcg gtacgacgat gcgatcgcgg
     1141 aggcccgccg ggccgtggag ctcgagccgt ggaacgccgc cacgcggggg aacctcgccg
     1201 ccgcctactg cggctgcggg cggttcgacg aggggatccg cgagtaccgg acggcggtgg
     1261 agatcgatcc cgggaaccgg aggctgcagg cggggctcaa acgggcgtac atctcgcgcg
     1321 ggtcgcaccg ggaggttgcg ccttagcgca cctacgggtc ggtcagcgcc cgcacgacgg
     1381 cggagatgtc ccggtcgccg aatcccttgt ccctcgcgat tgcgaaaagg ccgcaggcgg
     1441 cctgggtcac gggtgtcggg atccccatcg ccttccccgt ctcaacggcc agccgcaggt
     1501 ccttatgcgc gtgcttcag
//
LOCUS       DASVJL010000198         6352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_592335, whole genome shotgun sequence.
ACCESSION   DASVJL010000198 DASVJL010000000
VERSION     DASVJL010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592335"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="VF357_04040"
     CDS             <1..1584
                     /locus_tag="VF357_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7523350.1"
                     /translation="LRHRAAVALISVAVMLSVIPLYGLIRQDYLPANADEGEFNVSVT
                     APQGTSLAGMDGIMNDIVAEIRAVPGVRMVLSTAGGGWIGGVSEGRAYVQLAPHKERV
                     FSLTRLVKSTAGGHPLAAFRGNRSQLQVMQEVRRRLRKYRDLRVAVRNITGFNIGGGS
                     FDIDFVLRGPDLKALSTYAESLRDRQESLGLLDADTTLRLDKPELRVEIDRARAADLR
                     VDPERVGTAVRLLVGGDEEVSRFKDPSNDEDYKVALRLREEDRADPATIGRLLVSREG
                     GGVAQLESVARIERVPSASRIDRLDRQREVRLRASVAPGFAMADRLEALRSAVAAMNL
                     PAAYTTRVSGRGRELERTFVEFVWAFALSIVFMYMILASQFENLVHPCTILLSIPLCV
                     PFALLSIWLTGNTLNLYSALGILVLFGVVKKNAILQIDHMNNLRAGGMDRESAILQGN
                     RDRLRPILMTTLTFVAGMLPLAVGSGPGAEERRVIAVVVIGGQTLSLLLTLLATPVVY
                     SLLDDLGLVFRRRKGFAIR"
     gene            1645..2049
                     /locus_tag="VF357_04045"
     CDS             1645..2049
                     /locus_tag="VF357_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523351.1"
                     /translation="MEPSKIVARYCDGRTLKGTTQNFFPNRPIFHVNRPGGTGPGDVV
                     EVSLEDLKAIFFVRDFTGDTKHVERKQLAAGERTQGRLIEVTCKDGEVIVGTTTGYDP
                     KRPGFFLFPIDPSANNLRVFLVTSAVSTARFL"
     gene            2280..3554
                     /locus_tag="VF357_04050"
     CDS             2280..3554
                     /locus_tag="VF357_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7523352.1"
                     /translation="MKRSRTPEFVSGPPVRPFVSLFLLLLIATVSLPACRNAATSATL
                     PPTVDVLQVLRRDVPIFQEWIGTADGMVNATIRAQVTGYLIRQNYKEGDFIRNGQVLF
                     EIDPRTFQAALAQAEAQESQAAGQLAQAEAQHTNTRANLERVRPLADQNAVSKKDLDD
                     AIGAEGSTQAAVVAARANVAAARAAVEKARLDLGFTKITSPIAGIAGIAKAQVGNLVG
                     PAQGGELTTVSKIDPIKVYFTTSEQAYIVSMRQFSTETTAFERGKELPYDLILADGSL
                     FPHKGKFYAVDRQVDDRTGTLRLAVLFPNPGNLIRPGQFARVRMAAGMKKGALLVPQR
                     AVSELQGSYQVAVVGPDNRTEIRTVKAAERIGALWVIDEGIKPGERVVVEGIQKVRQG
                     APVTPKPLSPELRAKLEALPNAEGRTVPTVAK"
     gene            3560..>6352
                     /locus_tag="VF357_04055"
     CDS             3560..>6352
                     /locus_tag="VF357_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7523353.1"
                     /translation="MYKFFINRPIVAIVIAVITVIVGLVSMAGLPVAQYPNIVPPEIR
                     LSTVYVGADAQTVEQSVATPIEQQMSGVDGMNYIYSLNANDGSLRMIVDFDVKTDPNT
                     DHVLSQMRASQAESQLPIDVRNYGVTVQKSPAAPLVIYALSSPKGTYDGTFLANYAYI
                     NLNDQLTRIKGIGSVTIFGAGQYAMRCWVRPDILAKLNITVTEIVDALQKQNAVNPAG
                     QVGGEPIPPGQEFTYAIRAQGRLVAPEEFGRIVLRANPDGSLVRLRDVARIELGAQDY
                     GINGRFNGKPAALVALYQTPGSNALEAVEGSRKAMARIKASFPSDLDYSVALDTTLSV
                     TEGIREIVETLFIAMVLVIIVVFVFLQGWRATLIPAVAVPVSLIGTFAFFPLLGFSIN
                     PIALMGMVVAIGLVVDDAIVVVEAVERHIEEGMAPREATLAAMEQVAGPVMAIALVLA
                     AVFLPTIFIPGITGRLFQQFAVTIAISVLLSAFNALTLSPALSALLLRPRKATGGPLG
                     AFYDRFNRMFGRATDGYVRVSRGVIRKAAIGMAGLAAIALVSGLLGKGIPGGFLPEED
                     QGYLYAGIQLPLAASLQRTQEATRQVEKIILDTPGVRGCTTINGFNLLSQVRNTYSGF
                     FFIALKPWSERKRPEEQAAGIQARINRELGRLPQGVAFSFSPPAIQGIGTSGGVTFML
                     EDRAGRDIGVLAENTEKFIAAARKRPELARVTTTLLPSVPQVFVAVDRAKVLSEGVDL
                     GEVYRTLQSFMGGLFVNYFNRFGRQWQVYVQAEGEYRTDAAKLGQFYVRNSHGDAVPL
                     SALTRVERREGPEFIMRYNLYRSAQINAAATPGYSSGQAMAALEEVFAQTMPSDMGFD
                     YLGMSFQEQKAREGVPAVAVFGLSLLFVFLILAALYESWSLPFSVLLGTPIAVFGALA
                     ALMLRGMEFNIYGQIGL"
BASE COUNT         1092 a         2091 c         2084 g         1085 t
ORIGIN      
        1 ctccgccacc gggcggcagt ggcgctgatt tcggtggccg tcatgctttc ggtcatccct
       61 ctgtacggcc tgattcgcca ggattacctc ccggccaacg cggacgaggg ggagttcaac
      121 gtgagcgtga ccgccccgca aggtacgagc ctggccggca tggacgggat catgaacgac
      181 atcgtcgccg agatccgggc cgtgccgggg gtgaggatgg tgctttcgac cgccgggggc
      241 gggtggatcg gcggggtgag cgaggggagg gcgtatgtcc agcttgcccc gcacaaggag
      301 cgcgtcttct cgctcacccg gctggtgaag agcaccgcgg gcgggcaccc cctcgccgcg
      361 ttccgcggga accgttccca gcttcaagtg atgcaggagg tgcgtcggag gctccgcaag
      421 tatcgcgatc ttcgcgtcgc ggtccggaac atcaccggat tcaacatcgg tggcggaagc
      481 ttcgatatcg acttcgttct caggggaccc gatctcaagg cgctttcgac atacgcggag
      541 agcctgcgcg accgccagga atccctcggg cttcttgacg ccgacacgac gctccggctc
      601 gacaagcccg agctgcgggt cgagatcgac cgggcccgcg ccgcggatct tcgcgtcgac
      661 ccggagaggg tcgggacggc ggtcaggctc ctggtcggcg gagacgagga ggtctcgcgc
      721 ttcaaggatc cttccaacga cgaggactac aaagtggcgc tccggctccg cgaggaggac
      781 cgggcggacc ccgcgacgat cgggcggctc ctggtctcgc gggagggcgg aggcgtggcg
      841 cagctcgaaa gcgtggcgcg gatcgagcgg gtgccgagcg cctcgaggat cgaccgtctg
      901 gaccggcagc gcgaggttcg cctccgggcc tcggtggcgc ccgggttcgc gatggccgac
      961 cggctggagg cgctgcgttc cgcggtggcc gcgatgaacc tgcccgccgc ctacaccacg
     1021 cgcgtctccg ggcgggggcg ggagctcgag cggacgtttg tcgagttcgt ctgggccttc
     1081 gccctgtcga tcgtcttcat gtacatgatc ctcgcctccc agttcgagaa cctggtccac
     1141 ccctgcacca tcctgctctc gatcccgctc tgcgtcccct tcgccctgct gtcgatctgg
     1201 ctcacgggga acactttgaa cctctactcg gcgctcggga tcctcgtcct gttcggcgtg
     1261 gtcaagaaaa acgccattct ccagatcgac catatgaaca acctgcgggc gggagggatg
     1321 gaccgggagt cggcgatcct ccaggggaac cgggaccggc ttcggccgat cctcatgacg
     1381 acgctcacct tcgtggccgg catgctcccg ctggccgtcg gctccggccc gggcgccgag
     1441 gagcgacgcg tgatcgccgt cgttgtcatc ggcggccaga cgctctcgct cctgctcacg
     1501 ctgctcgcga caccggtcgt ctactcgctg ctggacgacc tggggcttgt cttccggcgg
     1561 cgcaagggat ttgccatccg ttgatatttt tgatggaatg aatcgaagtc aggtcgcttc
     1621 gaaccgaagc gctgggggga ccccatggaa ccatcgaaaa tcgtcgccag gtattgcgac
     1681 gggagaacct tgaagggcac cacgcagaat tttttcccca acagaccgat ttttcacgtc
     1741 aaccgcccgg gagggaccgg tcctggagac gttgtcgagg tgagcctcga ggacctgaag
     1801 gcgatcttct tcgtccggga tttcacgggg gacacgaagc atgtcgaaag aaaacagttg
     1861 gctgccggag aacggacgca gggccggctg atcgaggtca cctgcaagga cggcgaggtg
     1921 atcgtgggga cgacgaccgg ctacgacccg aagcgaccag gctttttcct gttcccgatc
     1981 gacccgtcgg ccaacaacct gcgggtcttc ctggtgacca gcgccgtcag caccgcccgg
     2041 ttcctctgaa aagaatcgag aaataaacga atatcaatct gttaccgcac ccttgtcttt
     2101 ccgcaaccgg gaagataatc tcccttgttg tgccggtaaa atctaaaacc ttattttatt
     2161 tctcgttgaa gtagcgtaga attcgtcggg ccgttcatct gccaggaagc gtgtggcgtc
     2221 cgggatgaat cgaatcggta actaggatca ataaggatgg taaggttggg agggaaacca
     2281 tgaagagatc cagaaccccc gaatttgtat ccggccctcc cgtgcgaccc ttcgtctcct
     2341 tgtttctcct cctcctcatt gcgacggtat ccttgcccgc ttgccggaat gccgctacga
     2401 gcgcaacgct gccgccgacg gtggacgtcc tgcaggtcct ccggcgggac gtcccgatct
     2461 tccaggagtg gatcggcacg gcggacggca tggtcaacgc caccatacgg gctcaggtca
     2521 cggggtacct catccgccag aactacaagg aaggcgactt catccggaac gggcaggtcc
     2581 tgttcgaaat cgacccgcgg accttccagg ccgcgctggc gcaggccgaa gcccaggaga
     2641 gccaggcggc gggccaactc gcgcaggcgg aggcgcagca caccaacacc cgggccaacc
     2701 tcgagcgggt caggccgctg gccgaccaga acgcggtgag caagaaggat ctcgatgacg
     2761 ccatcggcgc cgagggatcg acgcaggcgg ccgtcgtcgc cgcgcgcgcc aacgtcgccg
     2821 ccgcgcgggc ggcggtggag aaggcgcggc tcgacctcgg cttcacgaag atcacctccc
     2881 ccatcgccgg cattgccggc atcgccaagg cccaggtcgg gaacctcgtc ggcccggcgc
     2941 agggagggga gctgacgacc gtctcgaaga tcgaccccat caaggtctat ttcaccacca
     3001 gcgaacaggc gtacatcgtc tccatgaggc agttctcgac ggaaacgacg gcgttcgagc
     3061 gcggcaagga gctcccgtac gatctgattc tcgcggacgg gtcgctcttc ccccacaagg
     3121 ggaagttcta cgccgtcgac cggcaggtcg acgacaggac gggcacactc cggctggcgg
     3181 tcctgttccc gaacccgggg aacctgatcc ggcccgggca gttcgcccgc gtccgcatgg
     3241 cggcgggaat gaagaagggc gctctcctcg ttccccagag ggcggtgtcc gagctccagg
     3301 gcagttacca ggtggcagtg gtcggccccg acaaccggac ggagatccgc acggtcaagg
     3361 ccgcggagcg catcggagcc ctgtgggtga tcgacgaagg gataaagccc ggggaacgcg
     3421 tcgtggtcga ggggatccag aaggtaaggc agggggcccc ggtcactccg aaaccgcttt
     3481 cgcccgagct tcgggcgaaa cttgaagcac tcccgaacgc cgaaggacga accgtcccga
     3541 ccgtggcgaa gtagcctcct tgtacaaatt cttcatcaac cgcccgatcg tggcgatcgt
     3601 gatcgcggtc atcacggtca tcgtcggcct ggtatcgatg gcggggctgc cggtggcgca
     3661 gtacccgaac atcgttcccc cggagatccg gctgagcacg gtttacgtgg gcgccgacgc
     3721 gcagaccgtc gagcagtcgg tcgccacccc catcgagcag cagatgagcg gcgtggacgg
     3781 catgaactat atctattccc tcaatgccaa cgacggttcc ctgcggatga tcgtcgactt
     3841 cgacgtgaaa accgacccga acaccgacca cgtcctgtcc cagatgcgcg catcccaggc
     3901 ggagtcgcag ctgccgatcg acgtgagaaa ttacggcgtc accgtgcaga agtcccctgc
     3961 ggcgccactc gtcatctacg ccctctcctc tcccaagggg acctacgacg gcaccttcct
     4021 ggccaactac gcctacatca acctgaacga ccaactgacc cggatcaagg ggatcggcag
     4081 cgtcacgatc ttcggcgccg gccagtacgc catgcgctgc tgggtgcgcc cggacattct
     4141 cgccaagctc aacatcacgg tcaccgaaat cgtcgacgcg ctccagaagc agaacgccgt
     4201 caaccccgcc ggacaggtcg gtggcgaacc gatccccccc gggcaggagt tcacctacgc
     4261 gatccgggcc caagggcggc tggtcgctcc ggaggagttc ggccggatcg tcctgcgagc
     4321 caatcccgac ggctcgctcg tgcggttgcg ggacgtcgcg cggatcgagc tgggcgccca
     4381 ggactacggc atcaacggac ggttcaacgg gaagccggcg gcgctcgtcg cgctctacca
     4441 gactcccggc tcgaacgccc tggaggcggt cgaggggagc aggaaagcga tggcccggat
     4501 caaggcgtcg tttccgtccg acctcgacta ctctgtcgca ctcgacacca ccctcagcgt
     4561 caccgagggg atccgggaga tcgtcgagac gctgttcatc gcgatggtgc tcgtgatcat
     4621 cgtggtgttc gtcttcctcc aggggtggcg cgccacgctg atccccgccg tcgccgtgcc
     4681 ggtctcgctc atcggaacct tcgccttctt ccccctcctc gggttttcca tcaacccgat
     4741 cgccctgatg gggatggtcg tggccatcgg cctggtggtg gacgacgcca tcgtggtcgt
     4801 ggaggcggtg gagcgccaca tcgaggaggg gatggcccca cgggaggcga ccctcgcggc
     4861 gatggaacag gtcgccggcc cggtgatggc catcgcgctc gtcctggcgg cggtcttcct
     4921 ccccaccatc ttcatccccg gcatcacggg gcggctcttc cagcagttcg ccgtgaccat
     4981 cgccatctcg gtgctccttt cggcgttcaa cgccttgacg ctgagccctg cgctctcggc
     5041 gctgctcctg cggccgcgaa aggcgacggg gggcccgctg ggcgcttttt acgatcgatt
     5101 caaccgcatg ttcggtcgcg ccaccgacgg gtacgtccgc gtcagccggg gcgtcatccg
     5161 caaggcggcg atcgggatgg cggggctcgc cgcgatcgcg ctggtttccg gcctcctcgg
     5221 aaaggggatc ccgggcgggt tcctgccgga agaggatcag gggtacctgt acgccggcat
     5281 ccagctcccc ttagccgcct cgctccagcg gacccaggag gccacgcgcc aggtggagaa
     5341 gatcatcctc gacacccccg gcgtcagggg ctgcaccacg atcaacggct tcaaccttct
     5401 cagccaagtg cgcaatacct acagcggctt cttcttcatc gcgctgaagc cgtggtccga
     5461 acggaagcgc cccgaggaac aggccgcggg gatccaggcc cggatcaacc gcgagctggg
     5521 ccggcttccg cagggagtcg ccttctcctt ctccccaccg gccatccagg ggatcggaac
     5581 ctcgggcggc gtgaccttca tgctggagga ccgcgccggc cgggacatcg gcgtcctggc
     5641 ggagaacacg gagaagttca tcgccgccgc ccggaagcgc ccggagctgg cgagggtgac
     5701 caccactctc ctgcccagcg tgccgcaggt gttcgtggcc gtggacaggg ccaaggtgtt
     5761 gagcgaggga gtggacttgg gcgaggtgta ccgcacgctc cagtcgttca tgggaggtct
     5821 gttcgtcaac tatttcaacc ggttcggacg ccagtggcag gtgtatgtcc aggcggaggg
     5881 ggagtaccgg acggacgccg ccaagctcgg ccagttctac gtgcgcaaca gccatggcga
     5941 cgccgtgccg ctgtcggcgc tgacgcgggt cgaacggcgc gaggggccgg aattcatcat
     6001 gcgctacaac ctctaccggt cggcccagat caacgccgcc gccacgccgg ggtacagctc
     6061 ggggcaggcg atggcggcgt tggaggaggt cttcgcgcag accatgccgt ccgacatggg
     6121 cttcgactat ctcggaatgt cgttccagga gcagaaggcg cgcgaagggg tgccggcggt
     6181 cgccgtcttc ggcctgtcgc tcctcttcgt gttcctgatt ctcgcggcgc tgtacgagag
     6241 ctggtcgctg ccgttcagcg tgctcctggg aacccccatc gccgtcttcg gcgccctcgc
     6301 ggccctcatg ctccgtggga tggagttcaa catctacggc cagatcgggc tc
//
LOCUS       DASVJL010000199         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_874842, whole genome shotgun sequence.
ACCESSION   DASVJL010000199 DASVJL010000000
VERSION     DASVJL010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874842"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VF357_04060"
     CDS             complement(<1..353)
                     /locus_tag="VF357_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX7523354.1"
                     /translation="MIERYTRPEMAAIWQDENRFRIWLDIEILAMEAMVRQGWIPADA
                     LARVRKKASFDVARINEIEKKVKHDVIAFLTSVAEHIGDDSRFLHVGMTSSDVLDTAF
                     AVQMRQALGLLIREA"
     gene            complement(366..800)
                     /gene="dut"
                     /locus_tag="VF357_04065"
     CDS             complement(366..800)
                     /gene="dut"
                     /locus_tag="VF357_04065"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX7523355.1"
                     /translation="MIRVRLLRGGDDPLPAYQTAGSAGMDLKADVDGEIVIPPMGRAK
                     IPTGIALSMPTGVEGQVRPRSRLAIRHGVTCLNAPGTIDSDYRGEVCVILANFGEEPF
                     PVRRGDRVAQIVFSRTLRARLEVVPDLEETPRGEGGFGHTGR"
     gene            complement(820..>1616)
                     /locus_tag="VF357_04070"
     CDS             complement(820..>1616)
                     /locus_tag="VF357_04070"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7523356.1"
                     /translation="HPLGFPVQGTAESVGRFDRDRLKGYFDDRFRRTGIVVTAVGNLR
                     HAAVAEAFERALSSLPLGESLVPFPPTAPVRGTFLKRRPIEQVHLCLGAPGVSRRSER
                     IYAMDVLNAILGGSSSSRLFQQVREERGLAYSVGSSLSAYADAGVLDIYAGTGRENAA
                     EVLSVAGDVVDALQRGGVTDDEVAFGKELIKGNTLLSLENTGYRMSCLAMNEMFLSRL
                     EPPEAILERVDAVTPEEVRALASEVLRRDRFTLAAVGDLPDTGLSF"
BASE COUNT          254 a          601 c          511 g          250 t
ORIGIN      
        1 tccgcctcgc ggatcaggag cccaagggcc tgccgcatct ggacggcgaa ggcggtgtcc
       61 agaacgtcgg agctcgtcat ccccacgtgg aggaagcggg agtcgtcgcc gatatgttcg
      121 gccacggagg tcaggaaggc gatgacgtcg tgcttgacct ttttctcgat ctcgttgatc
      181 cgggcaacgt cgaaggacgc ctttttccgg acccgcgcga gggcgtccgc ggggatccac
      241 ccctggcgga ccatcgcctc catggcgagg atctcgatgt cgagccagat ccggaaccgg
      301 ttttcgtcct gccagatggc cgccatctcc ggccgcgtgt accgctcgat cacctcgtca
      361 ccccctcatc tccccgtgtg cccgaagccg ccctcgcccc gcggggtttc ttcgaggtcc
      421 ggcaccacct cgaggcgcgc ccgcagcgtc cgggaaaaga cgatctgcgc gacccggtcc
      481 ccgcgccgga cggggaacgg ttcctccccg aagttcgcga ggatcacgca cacctcgccg
      541 cggtagtcgg agtcgatcgt ccccggcgcg ttgaggcacg tcacgccgtg gcggatcgcc
      601 aggcggctgc gcggccgcac ctgaccctcg acccccgtcg gcatcgacag ggcgatcccc
      661 gtcgggatct tcgccctgcc catcggcggg atgacgatct cgccgtcgac atcggctttc
      721 aagtccattc cggccgaccc ggccgtctgg tacgcgggga gcggatcgtc tccaccgcgc
      781 agcaaccgca cccggatcat gcattccgcg tccatcgcgc taaaaagata gtcccgtgtc
      841 gggcaggtca ccgacggccg ccagcgtgaa cctgtccctg cggagcacct ccgacgcgag
      901 cgcgcgcact tcctccgggg tcaccgcgtc cacgcgttcg aggatcgcct ccgggggttc
      961 caggcgggag aggaacatct cgttcatcgc aaggcacgac atccggtacc ccgtgttttc
     1021 cagcgaaagc agggtgtttc ccttgatgag ctcctttccg aacgcgacct cgtcgtccgt
     1081 gaccccgccc cgctggaggg catccaccac gtcgcccgcg acggagagca cctcggcggc
     1141 gttctcgcgc cccgtgccgg cgtagatgtc gaggaccccg gcgtccgcgt aggcggacag
     1201 ggaagacccg acggagtacg ccaacccgcg ctcctcgcgg acctgctgga agaggcggct
     1261 gctggaactg cccccgagga tcgcgttcag cacgtccatc gcgtaaatcc gctcgcttcg
     1321 ccgcgaaacg ccgggagcgc ccagacacag gtgcacctgc tcgatgggcc tccgcttgag
     1381 gaacgtcccc cgcaccggcg ccgtgggagg aaagggaacc agcgactccc cgagcgggag
     1441 cgaggagagc gcgcgttcga acgcttccgc caccgccgcg tgccggaggt ttcccaccgc
     1501 cgtcaccacg atccccgtcc gccggaaccg gtcgtcgaaa taccccttga gccggtcccg
     1561 gtcgaaccgc cccacgctct ccgcggtccc ctgcacggga aaccccagcg ggtgcc
//
LOCUS       DASVJL010000200         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1532240, whole genome shotgun sequence.
ACCESSION   DASVJL010000200 DASVJL010000000
VERSION     DASVJL010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532240"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1161
                     /locus_tag="VF357_04075"
     CDS             295..1161
                     /locus_tag="VF357_04075"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX7523357.1"
                     /translation="MGKLERKIGKIRKEVFSNLTRWQITQLARHPNRPYLLDYVNLCF
                     KNFVELHGDRAFRDDPPLVCGFAELDGHRVVLIGQQKGRNTNEKIHRNFGMANPEGYR
                     KALRVMKMAEKFRLPVITFIDTPGAFPGIGAEERGQSEAIARNLLEMSHLKTPIVVAV
                     IGEGGSGGALAIGVGDEILMMEYSVYSVISPEGCASILWRDTAKAEVAAEMMKITAPD
                     LKKFGVIDRIIPEPLGGAHRDHKAAGDALKAALLETLPPLQSMPIPQLLERRYRKFRE
                     MGAMKRKAGGPA"
     gene            1158..2231
                     /gene="pheA"
                     /locus_tag="VF357_04080"
     CDS             1158..2231
                     /gene="pheA"
                     /locus_tag="VF357_04080"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX7523358.1"
                     /translation="MTQDRLKELRVEIDATDDRILELLNRRAKAAIEVGLIKKERNLN
                     VYVPEREVEILRRLTAVNEGPFPNDGLKAIYREIISASLSLEKPLSVAFLGPKATFTH
                     LACLKHFGESAEYVPQINLSGVFDAVDRNAADFGVVPIENSSEGIVSNTLDMFVDYHL
                     LICGEILVEVVHDLMNVTGDIDHVRKVYSHPHAIAQCRGWLERNLREVPVFDVESTAR
                     AAELAADDPAAAAIAGEAAAKIYGLMAIRKRIQDNTNNFTRFIILGKQASVPTGDDKT
                     SILFSGRDEVGALYLMLQPFAKHNVNLTKIESRPVKTKAWEYLFFLDMSGHLTDTPVA
                     QALEDLKERAQFIKILGSYPRAI"
BASE COUNT          468 a          754 c          738 g          367 t
ORIGIN      
        1 cgcaacggaa atgccgttgt tgatcgccaa gctcgcccca ccggaagcgg cgacaacgaa
       61 attgcccgat cgattgaagg taacatcgcc aacggtgcgg ctcacggtgt ccaccacagc
      121 ggtcgctccc gcgcccgcgc cagcgaagat cgcaccggca cctccatcgg ggatgctctg
      181 tggatcccaa ctggttgagg atgaccagtt gccgcttgga ttcgcgttcg tccaaaccgc
      241 ctcgagcagc tgaagcgcct cgacgacggg acggacaagg gcgtcaagga agagatcggg
      301 aagctggaac ggaagatcgg gaagatccgc aaggaggtct tctccaacct cacgcgatgg
      361 cagatcaccc agctcgcgcg ccacccgaat cggccgtacc tgctcgacta cgtcaacctc
      421 tgcttcaaga atttcgtcga actgcacggg gaccgggcgt tccgcgacga tccgcccctg
      481 gtgtgcggtt tcgccgagct cgatgggcat cgggtcgtcc tcatcggaca gcagaagggg
      541 cgcaacacca acgagaagat tcatcgcaat ttcggcatgg cgaatccgga ggggtaccgc
      601 aaggctctcc gcgtcatgaa gatggcggag aagttccgcc tccccgtcat caccttcatc
      661 gataccccgg gggccttccc cgggatcggc gccgaggagc gcgggcagtc cgaggcgatc
      721 gcgcgcaacc tcctggagat gtcccacctc aaaaccccga tcgtcgtggc cgtcatcggc
      781 gaggggggga gcggcggggc gctggccatc ggcgtggggg acgagatcct gatgatggaa
      841 tattccgtct actccgtcat ctcccccgag ggatgcgcct cgatcctctg gcgcgacacg
      901 gccaaggccg aggttgcggc ggagatgatg aagatcacgg ccccggacct gaagaagttc
      961 ggggtcatcg accggatcat cccggagccg ctgggcggcg cgcaccgcga ccacaaggcg
     1021 gccggagacg cgttgaaggc ggcgctgctg gagacgcttc ctccgctcca gtccatgccg
     1081 atcccgcagc tcctcgagcg gcgctaccgg aagttccggg agatgggggc gatgaagcgg
     1141 aaagccgggg gaccggcttg acgcaggacc gcctgaagga gctccgcgtc gaaatcgacg
     1201 ccaccgacga ccggatcctc gagctgctga accgccgggc gaaggcggcg atcgaggtcg
     1261 ggttgatcaa gaaggagcgc aacctcaacg tctacgtccc cgagcgggag gtggagattc
     1321 tccggcgcct gaccgccgtg aacgaggggc cctttcccaa cgacgggttg aaggcgatct
     1381 accgggagat catctccgcg tccctctccc tcgaaaagcc gctctccgtg gcgttcctgg
     1441 gccccaaggc caccttcacc cacctcgcgt gcctgaagca tttcggcgag agcgccgagt
     1501 acgttcccca gatcaatctc tccggggtgt tcgacgcggt cgaccggaac gcggccgact
     1561 tcggcgttgt gccgatcgag aacagcagcg agggaatcgt cagcaacacc ctggacatgt
     1621 tcgtcgatta ccacctgctc atctgcgggg agatcctcgt cgaggtggtg cacgacctca
     1681 tgaacgtcac gggcgacatc gaccacgtga ggaaggtcta ctcccatccg cacgccatcg
     1741 cccagtgccg cggatggctc gagcggaacc ttcgcgaagt ccccgtcttc gacgtcgaga
     1801 gcacggcgcg cgccgcggag ctcgcggcgg acgacccggc ggcggcggcg atcgccgggg
     1861 aggcggcggc caagatctac gggctgatgg cgatcaggaa gcggatccag gacaacacga
     1921 acaacttcac ccggttcatc atcctcggaa agcaggcgtc ggtcccaacg ggggacgaca
     1981 agacctcgat cctcttctcc ggccgggacg aggtcggggc gctctacctg atgctgcagc
     2041 cgttcgcaaa gcacaacgtg aacctgacca agatcgagtc gcgccccgta aagacgaagg
     2101 cgtgggaata cctcttcttc ctcgacatgt cgggccacct caccgatacg ccggtcgcgc
     2161 aggcgctcga ggacctcaag gagcgggccc agttcatcaa gatcctgggc tcctaccccc
     2221 gggcgatctg aatgcccttt cttcattcgt tcaaagggct cgcgcccttg aacgaattgt
     2281 gcaattgaac gaacaagaaa ccgggaaagg aacacgcaca tgatcat
//
LOCUS       DASVJL010000201         6481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_218323, whole genome shotgun sequence.
ACCESSION   DASVJL010000201 DASVJL010000000
VERSION     DASVJL010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218323"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /gene="rfaQ"
                     /locus_tag="VF357_04085"
     CDS             complement(<1..918)
                     /gene="rfaQ"
                     /locus_tag="VF357_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HEX7523359.1"
                     /translation="MGGSLPGRDLRSIAVFMLRHIGDTLLSTPAFHALRSAFPFARIL
                     AVVNEGTQEMLEGNPDIDGILVFHRRRRNAGGVGRWREEADLVRSLRAFEAEVAVNLT
                     EGDRGAILSVISGARFRVGVTPNRKGFLGKEILFTHLCGPHDRYRHAVLRDLDVVAAA
                     GIPPADLRLRFSISDEDRAKAVRILREAGIAAGRPFAVVQPTSRWTFKCWTEDGMAGV
                     ISHLADLGFAPVVTSGPAPVEVAQAERIRERAGGRGISLAGRLSLKELGAVIGSARLF
                     VGVDSAPMHVSAAVGTPTVALFGPTGAFNW"
     gene            complement(908..1654)
                     /locus_tag="VF357_04090"
     CDS             complement(908..1654)
                     /locus_tag="VF357_04090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7523360.1"
                     /translation="MKITAIVITKNEEGNVGDCLASIPFADEVIVVDSGSDDRTEEIC
                     RRDPRVRWFREPWKGFGPQKNSALDKAESDWILSIDADERVTPELAKEIASLPLSESG
                     VDGYRIPRRSFFGKRWVRHGGWYPDHTIRLWRRAAGRFVDRSVHEVVRVEGKVGTLRG
                     DLLHHTYMDTTDFVGRMNRYSTLGAGELRKKGVRCTTFDLLFRPPFTFFRMYVLRLGF
                     LDGALGFRLAVLYAMYTFLKYAKLRDADGG"
     gene            complement(1651..2754)
                     /locus_tag="VF357_04095"
     CDS             complement(1651..2754)
                     /locus_tag="VF357_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX7523361.1"
                     /translation="MPDVRRILIVKLSAMGDVVHALPAVTYLRKAAPSAEIHWAVDTR
                     FAELLEGNPGIAKVVSLPIKEWKGRLGSPSTWREAGNAAEALRGGMYDLAIDIQGNIK
                     SGLVTYLSGAPLRYGFPREVARERQNLWFTNRRPPAVEEDRHVTQKILRVASAPFGGT
                     FATEELRGEVVNTAAETEAASLLLAGTLPGASPRLAVHPGTTWNTKRMGAAFWADTVR
                     IVRDAFPGLGVFLSWGTEKEREDCVAIRYLAGEGVALLPRLGYKALAAAYRACGHMIG
                     PDTGPLHLAAVVGAKTVSVFRGSDGRYAAPVGPDHRFFQAPLPCTACQIKGDNVCPRD
                     AECRATIKAAEVAAAMAEFLAGSGAAPAGGKAP"
     gene            complement(2747..3805)
                     /gene="waaF"
                     /locus_tag="VF357_04100"
     CDS             complement(2747..3805)
                     /gene="waaF"
                     /locus_tag="VF357_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX7523362.1"
                     /translation="MGFNDPPALLLVRATNWLGDAVMTTPALAAVREGFPDARIVLLA
                     RPLVADLLRHHPDVDEVMIYERPGRHEGALGRLRLAGELRRRRFDGALLLPNAFDAAL
                     IAFLGRIPERAGYPTDGRRILLTLPVPLTPGILERHEVEYYLCLLDGLGIPRPVPTVL
                     KLAVTEEEREGMATRLASLGIERGAPIVTINPGATYGSAKRWYPDRFAAVAEALSAEW
                     SARVVVVGSTAEAPLAGEIEAAMRNPPVNLAGKTTVRELMALLSLSSFLVTNDSGPMH
                     VGAALGVPLVAIFGPTDWRRTSPWTSLAKVVRVEIDCSPCKRRVCDRGHECMLGVTPE
                     MVIEAARQLLPGGPRAGA"
     gene            3901..3988
                     /locus_tag="VF357_04105"
     tRNA            3901..3988
                     /locus_tag="VF357_04105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3935..3937,aa:Ser,seq:tga)
     gene            4026..4118
                     /locus_tag="VF357_04110"
     tRNA            4026..4118
                     /locus_tag="VF357_04110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4061..4063,aa:Ser,seq:gct)
     gene            4165..4258
                     /locus_tag="VF357_04115"
     tRNA            4165..4258
                     /locus_tag="VF357_04115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4200..4202,aa:Ser,seq:gga)
     gene            4326..4847
                     /locus_tag="VF357_04120"
     CDS             4326..4847
                     /locus_tag="VF357_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523363.1"
                     /translation="MKKFTLLVAVLMMVAFAGAGCKKQQPPPPPMPPKGAPGQPGMPG
                     APHGEAGAPPVEKKVVVPDAVKGAWKAVKIEVEFKEKKSKKAFTIPLNSEFKVPDSDL
                     TLKVGNFLPHFTMAADQITSGSNNLENPAAQIEVFQGGKEVFHGWLFAKYPAVHPFTH
                     DKYGVGLLEGIKK"
     gene            complement(4899..5684)
                     /locus_tag="VF357_04125"
     CDS             complement(4899..5684)
                     /locus_tag="VF357_04125"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX7523364.1"
                     /translation="MGNRELLGFAEELARGGGGILRKSYGRQQTIHFKGEINLVTDVD
                     RESEGYILGRIRERFPDHGLLSEESPERRSPSPYRWIVDPLDGTTNYAHGYPCFCVSV
                     AVEHEGAVVAGAVYDPLLDESFTAAKGEGAFRNGERIAVSKIAELRKSLLATGFAYDV
                     NTSTDNNFDYFRAFVLTGQAIRRDGSAALDLCYLACGRFDGFWELKLKPWDTAAGILV
                     LHEAGGGSTRLDGGPYDIHQPDLLASNGLIHAQMVEVLRKAKK"
     gene            5794..5955
                     /locus_tag="VF357_04130"
     CDS             5794..5955
                     /locus_tag="VF357_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523365.1"
                     /translation="MRRFAKPLLLPLCVLLLLSSCASTKAAKEWKPGDTVICPHCGRE
                     FPLPEKLGK"
     gene            6050..>6481
                     /locus_tag="VF357_04135"
     CDS             6050..>6481
                     /locus_tag="VF357_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523366.1"
                     /translation="MKPTRAAIAAATLFCSVLIGARGETLAGHPLGTEDAGTQGKGNV
                     EAEFNLERQNGNAGTRILSLGNIITMGIAPKIDLAVAYAYDFTKAEDGTKSRAMGPVE
                     ATLKKVFIEGKDRLPTFGVKAGFSLPTSEGDQAALLATAIAQ"
BASE COUNT         1161 a         2270 c         2016 g         1034 t
ORIGIN      
        1 ccagttgaac gccccggtcg gtccgaagag cgcgaccgtc ggcgtgccga ccgcggccga
       61 gacgtgcatc ggcgccgagt cgacgccgac gaacagccgc gccgagccga tcaccgcgcc
      121 cagttccttg agcgacaggc ggcccgccag ggaaatgccc ctcccccccg cccgttcccg
      181 gatccgttcc gcctgcgcca cctccacggg cgcggggccc gacgttacca cgggagcgaa
      241 ccccagatcg gcgagatggg agatcactcc cgccatgccg tcctccgtcc agcatttgaa
      301 ggtccaccgg gaggtcggct ggacgacggc gaaggggcga cccgccgcga tccctgcctc
      361 ccggaggatc cggaccgcct tggcccgatc ctcgtcggag atggagaagc gcaggcgaag
      421 atccgccggc gggattcccg ccgcggccac gacatccaga tccctcaaga cggcgtgccg
      481 ataccgatcg tgaggtccgc acaggtgggt aaaaaggatc tccttcccga ggaacccttt
      541 ccggttggga gtgaccccga cacggaaccg cgctcccgag atcaccgaga ggatcgcgcc
      601 ccggtctccc tcggtaaggt tcacggccac ctcggcctcg aacgcccgca acgaccggac
      661 caggtccgcc tcctccctcc accgcccgac cccccccgcg ttcctgcggc gccggtggaa
      721 tacgaggatt ccgtcgatgt ccggattccc ttcgagcatc tcctgcgttc cttcgttgac
      781 cacggccagg atccgggcga agggaaacgc cgagcgcagc gcgtggaagg cgggcgtcga
      841 aaggagcgtg tcgccgatgt gccggagcat gaagaccgcg atcgagcgga ggtcccgccc
      901 cgggaggcta ccccccatcg gcgtctctca acttggcgta tttgaggaaa gtgtacatgg
      961 cgtagagaac cgccagacgg aacccgagcg ccccgtcgag gaatcccagg cggagtacgt
     1021 acatccggaa gaaagtgaac gggggccgga agagaaggtc gaacgtcgtg caccggaccc
     1081 ctttcttccg aagctctccg gctccgagag tggagtagcg gttcatccgt ccgacgaagt
     1141 cggttgtgtc catgtaggtg tggtgcagca gatcgccccg gagagtaccc acctttcctt
     1201 ctacccggac cacctcgtgc accgagcggt ccacgaagcg gccggccgcc cgccgccaca
     1261 gccggatcgt gtggtcggga taccatcccc cgtggcgaac ccatcgcttc ccgaagaaac
     1321 ttcttctggg gatccggtac ccgtccactc cggattcgga aagcggcagg gaggcgatct
     1381 ccttcgccag ctccggtgtg acgcgctcgt cggcgtcgat gctcaagatc cagtcggatt
     1441 ccgccttgtc gagagcgctg ttcttctgcg ggccgaatcc cttccacggc tcgcggaacc
     1501 agcgcaccct cggatccctg cggcagatct cctccgtccg gtcgtcgctg cccgagtcga
     1561 cgacgatcac ttcgtcggcg aacgggatcg acgccagaca gtcgccgaca ttcccttcct
     1621 cgttcttcgt gatgacgatg gcggtgatct tcatggagcc tttccaccgg ccggcgcagc
     1681 gcccgatccc gcgaggaact ccgccatcgc cgccgcgacc tccgccgcct tgatggtggc
     1741 gcggcactcc gcatcccgcg ggcacacgtt gtcccccttg atctggcacg ccgtgcacgg
     1801 cagcggcgcc tggaagaagc gatggtccgg ccccaccggc gccgcgtacc gtccgtcgct
     1861 cccccggaag accgaaacgg tcttcgctcc gacgaccgcc gcgaggtgca gcggccccgt
     1921 gtccggaccg atcatgtgac cgcacgcccg gtatgccgcc gccagcgcct tgtatccgag
     1981 gcgcggcagc agcgccacac cttcgcccgc gagatatcgg atcgcgacgc aatcctcccg
     2041 ctccttctcc gttccccacg aaaggaacac ccccaggccg gggaaggcgt cccgtacgat
     2101 tcgcacggta tccgcccaga aagcggcgcc catccgcttc gtgttccacg tggtgccggg
     2161 atggacggcc aggcgcggag aggcaccggg cagcgtcccg gcgagaagca gcgaagccgc
     2221 ttccgtctcc gccgcggtgt tgacgacctc cccgcggagt tcctccgtcg cgaacgtccc
     2281 gccgaacggg gcgctggcca cgcgcaggat cttctgcgtg acgtgccggt cctcttccac
     2341 cgccggcgga cggcggttcg tgaaccagag attctgtcgt tcccgcgcga cctcccgggg
     2401 aaatccgtac ctcagcgggg cgccggaaag gtacgtcacc aggccgctct tgatgttccc
     2461 ctggatgtcg atggcgaggt catacatccc cccgcgcagc gcttccgcgg cattccccgc
     2521 ttcccgccac gtggagggag atccaaggcg ccccttccac tccttgatcg gcagggaaac
     2581 caccttcgcg attcccgggt tcccctcgag aagctccgcg aaccgggtgt cgacggccca
     2641 gtggatctcc gccgacggag ccgccttgcg aagatacgtc accgccggga gcgcgtgcac
     2701 cacgtccccc atcgcggaga gcttgacgat caggatcctg cggacatcag gcaccggcgc
     2761 gaggcccccc cggcagcagc tgccgcgcgg cttcgatcac catttccggg gtcaccccga
     2821 gcatgcactc gtgtccccgg tcgcagaccc gccgcttgca cggcgagcag tcgatctcca
     2881 cccggacgac cttcgccagc gaggtccacg gggaggtgcg ccgccagtcg gtgggcccga
     2941 agatcgccac gagcgggacg ccgagggcgg ccccgacgtg catcgggccg gagtcgttgg
     3001 tgaccagaaa ggaggagagg gagagaagcg ccatcagttc ccgcaccgtc gtcttcccgg
     3061 cgaggttgac cggggggttg cgcatcgcgg cttcgatctc gccggccagc ggggcctccg
     3121 cggtcgatcc cacgacgacg acccgcgcac tccactcggc ggatagggcc tccgcgacgg
     3181 cggcgaaccg gtccgggtac caccgcttcg cggaaccgta cgtcgcgccc ggattgatcg
     3241 tcacgatcgg cgcaccccgc tctatgccga gggacgcaag acgcgtcgcc atcccctccc
     3301 tctcttcctc cgtcacggcg agtttcaaca ccgtcggcac gggtcgaggg atccccagcc
     3361 cgtcgagcag gcacagataa tattccacct cgtggcgttc gaggatccct ggggtcaacg
     3421 gaacgggcag ggtaaggagg atccgccgtc catcggtggg ataccccgcc cgttccggga
     3481 tccggccgag aaaggcgatc aacgccgcgt cgaacgcgtt tggcagaagc aaggccccgt
     3541 cgaagcggcg acggcgcagt tccccggcca gccggagccg accgagggcc ccctcgtgac
     3601 gcccgggacg ctcgtagatc atcacctcgt ccacgtccgg atgatggcga agcagatccg
     3661 ccacgagagg ccgcgcgagc aggacgatcc gggcgtcagg aaagccttcg cgaacggctg
     3721 ccaacgcggg tgtcgtcatc accgcgtcgc ccagccagtt cgtcgcccgg accagcaaca
     3781 acgccggtgg atcgttgaat cccatgattc aatatcatac catcggcgtt tgcggtcgtc
     3841 ccggaccccc tttttttgct ccctcgcggc gataggatct ggtataaata catgtttcct
     3901 ggagggatgg ccgagcggct gaaggcggcg gtcttgaaaa ccgctgggcg gcgacgcccc
     3961 gcaggttcga atcctgctcc ctccgccagc cttgggattg ccgcggcaga gagtttgttt
     4021 tgtacggaga ggtggccgag ccggctgaag gcaaccgcct gctaagcggt tgtacgggtt
     4081 aaaccgtacc gagggttcga atccctccct ctccgccatt atcaatgatc tttggaaccg
     4141 gtttttcgtg aggattgtcc gtacggagag gtgtccgagc tggccgaagg aacgcgactg
     4201 gaaatcgcgt aggcggctta tacccgcctc gagggttcga atccctccct ctccgccatt
     4261 tcccaacgat tcgaatcacc gcgtgcgcgg cagcacgcgc caaatcgaag gaggaacaag
     4321 caacgatgaa aaaattcacg cttctggtcg ccgttctcat gatggtcgct ttcgcgggcg
     4381 ccggttgcaa gaagcagcaa ccgcccccgc cgccgatgcc tcccaagggc gcgcccggtc
     4441 agcccggcat gcccggcgct ccgcacggcg aggcaggggc acccccggtc gagaagaagg
     4501 tcgtcgtccc cgacgccgtc aagggggcat ggaaagccgt caagatcgaa gtggaattca
     4561 aggagaagaa gagcaaaaag gcgttcacca tccccttgaa ctccgagttc aaggtccccg
     4621 actccgacct cacgctgaag gtcgggaact tcctgccgca cttcacgatg gcggccgacc
     4681 agatcacctc cgggtcgaac aacctcgaga atccggcggc acaaatcgag gtgttccagg
     4741 gagggaagga ggtcttccac gggtggctgt tcgccaaata ccccgcggtg cacccgttca
     4801 cgcacgacaa gtacggggtc gggcttctcg aagggatcaa gaagtaacgc gcgtaccacc
     4861 cgacggcgca ggggggacgg ccttccgggc cgccccccct attttttcgc cttccgcagc
     4921 acctccacca tctgcgcgtg gatgaggccg ttggaggcga gcaggtccgg ctgatggatg
     4981 tcgtacggcc cgccgtcgag ccgggtcgag cccccccccg cctcgtgcag gacgaggatc
     5041 cccgccgccg tgtcccacgg cttcagcttc aactcccaga atccgtcgaa ccgcccgcac
     5101 gccagatagc aaaggtccag cgccgcggaa ccgtcccggc ggatcgcctg cccggtcaac
     5161 acgaacgcac gaaagtagtc gaagttgttg tccgtggacg tgttcacgtc gtaggcaaag
     5221 ccggtggcca ggagcgattt ccgaagttcc gcgatcttcg agacggcgat ccgttccccg
     5281 ttccggaacg ccccctctcc tttcgccgcc gtgaacgatt cgtcgagcag gggatcgtac
     5341 accgcgcccg ccacgaccgc gccttcatgc tccacggcca cggagacgca gaagcacggg
     5401 tagccgtgcg cgtaattcgt tgtcccgtcg aggggatcga cgatccaccg gtacggcgac
     5461 ggggaacgcc gctcggggct ctcctccgac agcaacccgt gatccggaaa ccgttcccgg
     5521 atccgcccga ggatataccc ttccgattca cggtccacgt cggtcaccag gttgatctct
     5581 cccttgaagt ggatcgtctg ctggcgcccg taacttttcc ggaggatccc gccgcccccg
     5641 cgggccagtt cctcggcgaa ccccagcagt tcccggtttc ccatcgcatc cccccttttt
     5701 ccgcctgatg gaaccagcgc cgattataat atgcggatcg cgccgcgccc cgaaaagatg
     5761 catgagggaa gaacggcgca gggagacctt atgatgagac gattcgccaa accgttgctc
     5821 ctgccgctct gcgtcctgct cctcctttcc tcgtgcgcct cgacgaaggc cgcgaaggaa
     5881 tggaagccgg gcgacaccgt gatctgcccc cactgcggcc gcgagttccc gctcccggag
     5941 aagctcggca agtgatttcg cccggggccc accttgtgtt gaaaggaggc cgatccgctt
     6001 ggcaggcgta agtggcatca gaatccggaa gggaaagggc actctcggca tgaaaccgac
     6061 cagggcggct attgctgccg ccacgctttt ctgcagcgtc ctgatcgggg cacggggaga
     6121 gaccctcgcc ggtcaccctc tcggcacaga agacgcggga acacagggaa aagggaacgt
     6181 cgaagccgag ttcaacctcg agcggcagaa cggcaacgcc gggacgagga tcctctccct
     6241 cggaaacatc atcacgatgg ggatcgcccc gaaaatcgac ctcgccgtcg cctatgccta
     6301 cgacttcacg aaagcggaag acggcacgaa gtcccgggcg atgggtcccg tcgaggcgac
     6361 ccttaagaag gtcttcatcg aagggaagga ccggctcccg acgttcgggg tcaaggcggg
     6421 attctcgctc ccgacctccg aaggggatca ggccgcgctt ctggcgaccg ccatcgccca
     6481 a
//
LOCUS       DASVJL010000202         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_968951, whole genome shotgun sequence.
ACCESSION   DASVJL010000202 DASVJL010000000
VERSION     DASVJL010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968951"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VF357_04140"
     CDS             complement(<1..358)
                     /locus_tag="VF357_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7523367.1"
                     /translation="MLVGKRMTRNPKTVSPDDPLSFAAEILREHRFHHLPVVEGGRLV
                     GILSDTDLRNASFAAMPAEGEGGPAGDRPVREAMRTEVWSVIPDDSVEDALLILTREK
                     FGALPVLSGDRLVGIIT"
     gene            complement(623..>1721)
                     /gene="gdhA"
                     /locus_tag="VF357_04145"
     CDS             complement(623..>1721)
                     /gene="gdhA"
                     /locus_tag="VF357_04145"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEX7523368.1"
                     /translation="QFNSALGPYKGGLRFHPSVYLGIIKFLGFEQIFKNSLTGLPIGG
                     AKGGSDFDPKGKSDNEVMRFCQSFMTELWRIIGEHTDVPAGDIGVGGREIGYLFGQYK
                     RLTNKFEAGVLTGKGLVYGGSQVRTEATGYGCTYFVEEMLKGKKNGFKGKKAVVSGSG
                     NVAIYTLEKIHQLGGKVIAMSDSNGVIVDEKGCNLETIKQLKEVERRRIKDYCDYHRH
                     AKFLPGGNIWEVPCDVAMPSATQNEITGKDAKKLVKNGCIAVGEGANMPTTPDGVQVF
                     LSAGVLFGPAKAANAGGVATSALEMQQNAGREAWGFEETDAKLHQIMRNVYQICSEAA
                     DEFGSPGNFVVGANIAGFIKVARAMSAHGLV"
BASE COUNT          267 a          601 c          526 g          327 t
ORIGIN      
        1 tcgtgatgat ccccaccagg cggtccccgg agagcaccgg gagggcgccg aacttttccc
       61 gggtgaggat gaggagcgcg tcctcgaccg aatcgtcggg gatgacggac cagacctccg
      121 tccgcatcgc ctcccggacc ggccggtccc ccgccggccc cccttcccct tccgcgggca
      181 tcgcggcgaa cgaggcgttc cgcaggtccg tgtcggacag gatcccgacc agtctcccgc
      241 cctcgaccac cggaagatgg tggaaccggt gttcccgaag gatctccgcg gcgaacgaga
      301 gcgggtcgtc cggagaaacc gtcttcgggt tccgcgtcat ccgttttccg acgagcatcg
      361 gggggtcctc cgttttcatg ccgatcggcc cgagacggcc gggaaaggac gatccgcatc
      421 caatgtaacg catcccccgg gagatgtcca agtggcccca tgccgggcaa ggaatatggc
      481 atcggttatc gggggtaccg cagggagcgt actttgcaag ggagtggggc tgcatgggtg
      541 caagggaaat ctcccggaag gaaccggcgg ggaggcagag ccccccccgc cgggattgcg
      601 ttttcgcgta cgatccgcgc ggctacacca ggccgtgcgc tgacatcgcc cgggctacct
      661 tgatgaaccc ggcgatgttg gcgcccacca cgaagttccc gggggaaccg aactcgtcgg
      721 ccgcctcgga gcagatctgg tagacgttgc gcatgatctg gtggagcttg gcgtccgtct
      781 cctcgaaccc ccacgcctcg cgccccgcgt tctgctgcat ctcgagcgcc gaggtggcca
      841 cgccgcccgc gttggccgcc ttggcgggtc cgaagagcac acccgcggag aggaacacct
      901 gcacgccgtc cggcgtcgtc ggcatgttcg ccccttcgcc cacggcgatg cagccgtttt
      961 tcaccaactt cttcgcgtct ttcccggtga tctcgttctg ggtggcggag ggcatcgcca
     1021 catcgcacgg cacttcccag atgtttccgc cggggaggaa cttggcgtgc ctgtggtaat
     1081 cgcagtagtc cttgatccgg cgccgttcca cttccttcag ctgcttgatc gtctcgaggt
     1141 tgcatccctt ctcgtccacg atcacgccgt tgctgtcgct catggcgatc accttgccgc
     1201 caagctggtg gatcttctcc agcgtgtaga tcgccacgtt gcccgacccc gacacgacgg
     1261 ccttcttccc cttgaatccg ttcttcttcc ctttcagcat ctcctcgacg aagtaggtgc
     1321 agccgtagcc ggtcgcctcg gtgcgcacct ggcttccgcc gtagaccaac cccttccccg
     1381 tgaggacccc cgcctcgaac ttgttcgtca ggcgcttgta ctgcccgaac aggtagccga
     1441 tctcgcggcc gcccaccccg atgtcgcccg cggggacgtc cgtgtgctcg ccgatgatcc
     1501 gccacagctc cgtcatgaag ctctggcaga atcgcatcac ctcgttgtcc gacttcccct
     1561 tcggatcgaa gtccgatccc cccttggcgc cgccgatcgg cagcccggtc agggagttct
     1621 tgaagatctg ctcgaacccg aggaacttga tgatcccgag gtagaccgag gggtggaacc
     1681 ggagcccccc cttgtacggc ccgagggcgc tgttgaactg c
//
LOCUS       DASVJL010000203         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1063317, whole genome shotgun sequence.
ACCESSION   DASVJL010000203 DASVJL010000000
VERSION     DASVJL010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063317"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..760)
                     /locus_tag="VF357_04150"
     CDS             complement(155..760)
                     /locus_tag="VF357_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX7523369.1"
                     /translation="MGDYSATVVDPTDNVTFWTLQEYAQTLDPVTGASRWGTWWGKFH
                     PSDVLAPAGLTATADNAAQVILRWTDLATNETGYVVDRKVGATGTFGVLTSLPPNSVT
                     YTDNVGLVAGTTYLYRVGAVGSTGTSYSLEVPITIPRPPSSSGGGGGGGCLSITRSGG
                     EMSIGTSLFSVGLLLLPALAPGLRRFSRRRERDVPFRHPLC"
     gene            complement(715..2307)
                     /locus_tag="VF357_04155"
     CDS             complement(715..2307)
                     /locus_tag="VF357_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523370.1"
                     /translation="MDPGNSFHEHRVVRLLLRWMVPAAVILLAAASSGDSVGSEPTTS
                     TGVQVTMRVPFVRESIDEIRALAPLRERTVPENRAIPFHRIPRRSGTTGHAPLPFAAP
                     GLAAPFLASSPAPSAPVLDSSFAGLGNPPHSEGDLTPPDTMGAAGPNHLVSLLNSDFG
                     VFDKTTGAVRQKISLESFWKGGTIGTAAGQPANFPFDTKVLYDPSSDRFFAVTLGGTG
                     SPDSWILVAASATPDPTGSWNKWAIDADLDNNVQQFNNWADFPGFGLDGNNLYVSTNM
                     FSNSDSFQYGKVWVVPKAQLLSLSATANITWTELRDPPGSSFNMQPAHTFGAAPAEYF
                     LFEGSSSQLLMARIDNTSGTPVWHAPTGVNVAAYNSSDRLPGAPQLGLGADNTIDTTD
                     TRLLNVVYRNGSLWTVQTVQSPATTKTEVAWYQINPDTGAVPTQGRISDPARWYYYPS
                     IGVNRDNIAAIGFSGSSPTEHAGGYYTIIRQPDGAADPVSLLKAGEAPYYKTGKTLGI
                     PGGGTENRWGITAPRWSIRPTT"
     gene            complement(2319..>2996)
                     /locus_tag="VF357_04160"
     CDS             complement(2319..>2996)
                     /locus_tag="VF357_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523371.1"
                     /translation="LLAVFLPLVSGCLFGEKRRTFSPPDEELLRAKADPRLRKEAETG
                     GNESFAAIAVYRNDVFLHPSEALDQLSLTVLNEMGRTAILLLSPGQIVPLLKDPSLQK
                     AAWIGPQGLLARLDPSLELDLLTGFGAGTEDRDVTLLLRFVDVGGAGEERTVVDAGFR
                     VDTRAGPNWIATGPMSGLPKLLESDRITYIEKADPADKRENTAIHSHRIPRTGNDNGI
                     SIPPGDD"
BASE COUNT          513 a          930 c         1014 g          539 t
ORIGIN      
        1 ggcaactgga ggtcggcctc gtgccccctg ccgatcttga cctctttcgg ccccagccgc
       61 acctcgaccg tcatcccgtt cgaaatggta acggaaagcc tcgcgcccgg atcgcccacg
      121 atcatctccc ccctccaact cgtttcgcct tttcttagca aagggggtgc cggaacggga
      181 cgtctcgctc ccgtcgacgg gaaaaacgcc gcaatccggg agcgagtgcg gggaggagga
      241 ggagcccgac ggagaagagc gacgtgccga tcgacatctc gcctccggat cgggtgatgg
      301 aaaggcatcc gccgccaccg ccgcctccgg aagacgaggg cggtctcggg atcgtgatcg
      361 ggacctcaag ggaatacgac gtgccggtgg aaccgaccgc gccgacccga tagaggtacg
      421 tcgtaccggc aacgagcccc acgttgtccg tgtacgtgac actgttcggg ggcagggagg
      481 taaggactcc gaaggtcccc gtcgccccga ccttccgatc gacgacatag ccggtttcgt
      541 tcgtggcgag atcggtccag cggaggatga cctgggcggc gttgtccgcc gtggcggtca
      601 gcccggccgg cgcgaggacg tcggagggat ggaattttcc ccaccaggtt ccccatcgtg
      661 atgctccagt aaccggatca agagtctgag cgtactcctg aagggtccag aacgtcacgt
      721 tgtcggtcgg atcgaccacc gtggcgctgt aatcccccaa cgattctccg taccccctcc
      781 ggggatcccc agcgtctttc cggtcttgta gtagggggcc tcgccggcct tcagcaggga
      841 aaccgggtcg gccgctccgt ccggctgccg gatgatggtg taataccccc ctgcgtgttc
      901 tgtcggggag gatccgctga atccgatcgc cgcgatgttg tcccggttga caccgatgga
      961 aggatagtag taccagcggg ccgggtcgct gatccgtccc tgtgtcggaa ccgcccccgt
     1021 gtcggggttg atttgatacc aggcgacttc cgtcttggtc gtggccgggc tctgcacggt
     1081 ctggacggtc cacaaggaac cgttccggta caccacgttc aagaggcggg tgtccgtcgt
     1141 gtcgatcgta ttgtcggcgc cgagtccgag ttgcggggcg cccggcaggc ggtccgagga
     1201 attatatgct gcgacgttca cgccggtcgg cgcgtgccag accggggtgc cggacgtgtt
     1261 gtcgatccgg gccatgagaa gttggctcga ggatccctcg aagaggaagt attcggcggg
     1321 cgcagccccg aaggtgtggg cgggctgcat gttgaagctg cttcccggcg ggtcccggag
     1381 ctcggtccag gtgatgttgg cggtggccga aagcgaaagc agctgcgcct tggggacgac
     1441 ccagaccttc ccgtattgga aggaatccga gttgctgaac atgttggtgc tgacgtacag
     1501 gttgttcccg tccagtccga atcccgggaa atccgcccag ttgttgaact gctgcacgtt
     1561 gttgtccagg tccgcgtcga tggcccactt gttccatgag cccgtgggat cgggcgtggc
     1621 ggaggcggcc accaggatcc aggagtccgg agagcccgta ccacccagcg tcaccgcgaa
     1681 gaaccggtcg ctggacgggt cgtagagcac cttggtgtcg aacgggaaat tcgccggttg
     1741 acccgccgcg gttccgatgg tccccccctt ccagaacgat tcgagggaaa tcttttgacg
     1801 caccgccccc gtggttttgt cgaacacccc gaagtcgctg ttcaggaggc tcacgagatg
     1861 gttggggccc gccgcgccca tcgtgtcggg gggggtcagg tccccttcgg agtgaggggg
     1921 gttcccaagg cccgcgaagc tgctgtcgag cacgggcgcc gagggggcgg gagacgaagc
     1981 aaggaatggg gccgcaagcc ctggcgctgc aaacggcaac ggtgcatgtc ccgtcgtacc
     2041 ggatcgcctt ggaattcgat gaaaggggat ggcccggttc tccggtaccg ttcgctcccg
     2101 caagggggcg agcgcacgga tctcgtcgat cgattcccgg acgaacggga cacgcatcgt
     2161 gacttgcact ccggtcgacg tcgtcggctc ggatcccacg gaatcgccgg aagacgcggc
     2221 ggcgaggagg atcacggcgg cgggaaccat ccaccgaagg agcaggcgta caacgcggtg
     2281 ttcatggaaa gaatttccag ggtccattct gtcctccctc aatcgtcccc cggggggatc
     2341 gatatgccgt tgtcattccc ggtccgggga atcctgtggg aatggatcgc cgtattctcc
     2401 cttttgtccg ccggatccgc tttttcgatg taggtgattc gatcgctttc cagaagcttc
     2461 gggagccccg acatcgggcc ggtggcgatc cagttcgggc cggccctggt gtcgacgcgg
     2521 aatccggcgt cgacgacggt ccgctcctct cccgccccgc cgacgtccac gaaccggagc
     2581 aggagagtca cgtcacggtc ttcggtcccc gcgccgaagc cggtgagaag atcgagctcc
     2641 agcgacgggt caagccgggc caggagcccc tgcggtccga tccatgccgc cttttgcagg
     2701 gaagggtcct tgaggagggg aacgatctgc ccgggagaca gcagcaggat ggcggtgcgg
     2761 cccatctcgt tcaggaccgt cagcgacaat tggtcgagag cctccgacgg atggaggaag
     2821 acgtcgttgc ggtagaccgc gatcgccgcg aacgactcgt tcccgcccgt ctccgcctcc
     2881 ttgcgcaacc gcgggtcggc cttggcgcgg agaagttctt cgtccggggg agagaaggtc
     2941 cgtcgcttct ccccgaacag gcacccggag accaacggca ggaacaccgc gagcag
//
LOCUS       DASVJL010000204         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_969111, whole genome shotgun sequence.
ACCESSION   DASVJL010000204 DASVJL010000000
VERSION     DASVJL010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969111"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VF357_04165"
     CDS             complement(<1..423)
                     /locus_tag="VF357_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7523372.1"
                     /translation="MFVARRMKKKIVTAAPSTSLFEAQRQMREHTIRQVPVVSEDGTL
                     LGIVSDRDIRAAVLPADLVPGFTAGEAEKFMKNTPVERVMTRKVVTATLTDTLEDAIV
                     LLHDFRVNALPVVDAAGKIAGIITRTDVLEAFIDALGVG"
     gene            complement(448..1398)
                     /locus_tag="VF357_04170"
     CDS             complement(448..1398)
                     /locus_tag="VF357_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX7523373.1"
                     /translation="MKRVAWIYHPDYLLHTPSFEHPESPERLVAITDHLGGAGLIDRM
                     VPVTPEYPADADILPVHDREYLNRLENACRRGDITLDSEDTYLNRHSFNIALLSAGGA
                     IAGAKAVAEGTVDSAFCAIRPPGHHAGRDVGMGFCLLNNAAVAARYLQARHGVGKVLI
                     VDWDVHHGNGTQNIFLEDPTVFYFSIHEHPTFLYPGTGRRWEIGKGKGEGATLNAPMA
                     PGAGDAEYRTTFEQTLEPAVERFRPEIILVSAGFDAHRDDPLADLQVTEEGFRFMTRH
                     VLALADRFCGGKVVSILEGGYETSSLTSCIEIHVRELLGE"
BASE COUNT          230 a          512 c          512 g          274 t
ORIGIN      
        1 gcccacgccg agggcgtcga tgaacgcctc cagcacgtcg gtccgggtga tgatcccggc
       61 gattttcccc gcggcgtcca cgaccggcaa ggcgttcacc cggaagtcgt gcagcagaac
      121 gatcgcgtct tccagcgtgt cggtgagcgt ggcggtgacg acctttcggg tcatcacccg
      181 ctccacgggg gtgttcttca tgaacttctc cgcctccccg gccgtgaatc ccggcaccag
      241 gtccgcgggg agcaccgcgg cccgaatgtc ccgatcggac acgatcccca acagggtgcc
      301 gtcctccgag accaccggga cctgacgtat ggtgtgttcc cgcatctggc gctgcgcctc
      361 gaagagggac gtggacgggg ccgccgtcac gatctttttc ttcattctgc gtgcgacgaa
      421 cattggatca ccctccgtgg gccgggatca ttcgccgaga agctcccgga cgtgaatttc
      481 gatgcaggac gtgagcgagg aggtttcata ccctccctcg aggatcgaga cgaccttccc
      541 tccgcagaac cggtcggcca gcgcgagcac gtggcgggtc atgaagcgga acccctcctc
      601 cgtcacctgg aggtccgcca gcgggtcgtc gcggtgggcg tcgaagcccg ccgagacgag
      661 gatgatctcc ggccggaacc gctccacggc cggctcgagc gtctgttcga aggtcgtccg
      721 gtactccgcg tcccccgcgc cgggggccat cggggcgttg agcgtcgcgc cttccccctt
      781 ccccttgccg atctcccacc ggcggccggt gccggggtag aggaaggtgg ggtgctcgtg
      841 gatgctgaaa tagaagaccg tcgggtcctc gaggaagatg ttctgcgtgc cgttgccatg
      901 gtggacgtcc cagtcgacga tgaggacctt tcccacaccg tgcctcgcct ggaggtaccg
      961 ggccgcgacg gccgcgttgt tgaggaggca gaaccccatc cccacgtccc gtcccgcgtg
     1021 gtgccccgga ggccggatgg cgcagaaggc gctgtcgacc gtcccttccg ccaccgcctt
     1081 cgcccccgcg atcgcccctc ccgccgacag gagcgcgatg ttgaaggagt gccggttgag
     1141 gtaggtgtcc tccgagtcga gggtgatgtc gccgcgccgg caggcgttct ccagcctgtt
     1201 cagatattcc cggtcgtgga cggggaggat atccgcgtct gccggatact ccggcgtcac
     1261 cgggaccatc cggtcgatca ggccggctcc cccgagatga tcggtgatgg cgaccaggcg
     1321 ctccggggac tccgggtgtt cgaatgatgg cgtgtggagg aggtagtcgg ggtggtagat
     1381 ccaggccact cgcttcatga gcgctcccta cgcaccccgt cactatatca gattcgggct
     1441 atcggaacgt catgatctcc gacgccggct tgcgcggcgg tttcgggggc gccgcgtccg
     1501 gtttcccaac ggcgatgatc gccagcag
//
LOCUS       DASVJL010000205         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_500758, whole genome shotgun sequence.
ACCESSION   DASVJL010000205 DASVJL010000000
VERSION     DASVJL010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500758"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..461)
                     /locus_tag="VF357_04175"
     CDS             complement(138..461)
                     /locus_tag="VF357_04175"
                     /inference="COORDINATES: protein motif:HMM:NF017426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HEX7523374.1"
                     /translation="MDLRFSERAQGLLAEIHGENPSDTLVILIGGGCCENTAPILMKN
                     FRVGRSDQLLGEMDGIKVYAAAEMYPLVSATPSIIDVTDGRGGGSFSLEIPRGVRLTL
                     RPLPG"
     gene            complement(478..1953)
                     /locus_tag="VF357_04180"
     CDS             complement(478..1953)
                     /locus_tag="VF357_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7523375.1"
                     /translation="MGTVVQKVYDLFIGGKWTKSGSGKTIDVLNPATGDLLTRVPAGD
                     TRDVDAAVAAAREAFVTWGKTSAVERQGILLEIANRIQKRARDYAELETMNVGKPIRE
                     TMNIDIPAAIDHYRYFAGVLRNIQGVTQSIDPTMLHLTLREPLGVVGHILPWNFPLLL
                     AAWKLAPSLAAGNTVVMKPAEQTPLTLLELANDLRDVLPPGVLNVVTGYGPDAGAPLA
                     SHPGIRKVAFTGETTTGRLILQYASENLVPATMELGGKSPHIIFPDASVERAIEGVLI
                     GVFFNQGEVCSAGSRLFVHDAIYDSFVPKLVAKVKGIKVGDPMKEDTQLGAIVSREQM
                     EKVLSYVEIGKKEGATLLCGGTRPSDPALSKGFFVSPTIFGGVRNKMRIAQEEIFGPV
                     VSVIRWNDYDAMIAEANDTPYGLASGLWTENLPLAINTAKALQAGTVWINSYNSLYAG
                     APFGGYKKSGFGRECAFETLQYYTQTKSIFVSTAEKPMGLY"
     gene            2269..>2710
                     /locus_tag="VF357_04185"
     CDS             2269..>2710
                     /locus_tag="VF357_04185"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7523376.1"
                     /translation="MKAAVCESFGKPLVLSEVSAPSPGPAELLIRVSACGVCHSDLHL
                     IDGEWRQWGTRPPFIPGHEVTGVVEEAGPEARGFAPGDRVGVPWMQFACGECAPCLSG
                     AEMLCAAQRGTGVTVDGGYAEFVKAPAAFVHRIPEPLDLVDSAPL"
BASE COUNT          478 a          839 c          848 g          545 t
ORIGIN      
        1 gggagatgcc tgaacggaag gatgctgttt atcaatctgc tggacatgga gttcctggac
       61 atggagttcg atgacgaccc gatcgtgtcc ggggccgcgc ctggggacac cggatgaccc
      121 gatccccacg gtttcgtcta ccccggaaga ggtcgtagcg taagccggac gccgcggggg
      181 atctccagcg agaagctgcc ccctccccgc ccgtccgtca cgtcgatgat cgaaggcgtg
      241 gcggagacaa gggggtacat ctccgccgcg gcgtatacct tgatcccgtc catctccccg
      301 agaagttggt cgcttcgtcc cacgcggaag ttcttcatca ggatgggagc ggtgttctcg
      361 cagcagccac ccccgatgag gatcacgagg gtatccgacg ggttttcgcc gtggatctcc
      421 gccagaagtc cctgcgctct ttccgagaag cgaagatcca cggtcaagcc ccccttccta
      481 ataaaggccc atcggcttct ccgccgtcga gacgaagatg ctctttgtct gggtatagta
      541 ttgcagcgtt tcgaaggcgc actctcttcc gaaaccgctt ttcttgtacc cgccgaacgg
      601 cgccccggcg tagaggctgt tgtaactgtt gatccaaacg gtccccgcct gcaacgcttt
      661 tgcggtattt attgcaaggg gaagattctc cgtccagagc cccgacgcga gcccgtaggg
      721 ggtgtcgttg gcctccgcga tcatggcgtc gtaatcgttc caccggatca cggaaaccac
      781 ggggccgaag atctcctcct gcgcgatccg catcttgtta cggacgcctc cgaagatcgt
      841 cgggctgacg aagaatcctt tggagagggc cgggtcggac ggccgcgtcc cgccgcagag
      901 aagggtcgca ccctccttct ttccgatctc cacgtacgag aggacctttt ccatctgctc
      961 cctggagacg atcgctccca gttgggtatc ctccttcatc gggtcgccga ccttgatccc
     1021 cttgaccttg gcgaccagtt ttggaacgaa ggagtcgtag atggcatcgt gcacgaacag
     1081 ccgggatccg gcggagcaga cctccccctg gttgaagaag acgccgatca gcaccccctc
     1141 gatggcccgc tcgacgctcg cgtccgggaa gatgatgtgc gggctctttc cgcccagctc
     1201 catggtggcg gggacgaggt tctccgaggc gtactgcagg atgagccgcc ccgtcgtggt
     1261 ctcgcccgtg aaggccactt tccgtatccc cgggtggctc gcaaggggag cgcccgcatc
     1321 gggcccgtac ccggtgacga cgttcaggac ccccggcggg aggacgtccc gcaggtcgtt
     1381 ggcaagttcg agcagggtga ggggggtctg ctccgccggc ttcatcacca cggtgttccc
     1441 ggccgccagc gagggcgcga gtttccatgc cgcgagcagg agggggaaat tccacgggag
     1501 gatgtgcccg accacgccga gcggttcccg aagggtgagg tgcagcatgg tcgggtcgat
     1561 ggattgggta accccctgga tgttccgcag cacaccggcg aaataccggt aatggtcgat
     1621 cgccgccggg atgtcgatgt tcatcgtttc gcggatcggc ttcccgacgt tcatcgtttc
     1681 cagctccgcg tagtcccgtg cccgcttctg gatccggttg gcgatctcca gcaggatccc
     1741 ctgtcgctcg acggcggagg tcttccccca ggtgacgaac gcctccctgg cggccgcgac
     1801 cgccgcgtcg acgtcccggg tatcccctgc cggcacgcgg gtcagtaggt ctcccgtcgc
     1861 cgggttcaat acgtcgatgg tttttcccga gccggatttc gtccactttc ctccgatgaa
     1921 aagatcgtat actttctgta cgacagtccc catggaatcc ctccttgcag gaaatgataa
     1981 gaaaaaggta ccgcaatata catgcctaca ttacggtgtt ccctaagcaa gctgaagcaa
     2041 gagcatctat tggataaaaa agctttttct gcaaaggttc ccgttataac gaacaaaacg
     2101 attgtcgggg agatcttccc tggcgcaacg gtgggacgga attctaattt ttgggatttg
     2161 gttctcgtag ccgataatgt agcgaaccag cctggtggag agtatccttc aatcgttgaa
     2221 tcatcatggt cttcgagtca tgaggcagcc ggacggagat atcttccgat gaaagccgcc
     2281 gtctgcgagt cgttcgggaa gccgctcgtc ctctcggagg tttccgcgcc ttcccccggc
     2341 cccgccgaac ttctgatccg cgtgagcgcg tgcggagtct gccacagcga cctccacctg
     2401 atcgacgggg agtggagaca gtggggaacg aggcccccgt tcatcccggg gcacgaagtg
     2461 accggggtcg tcgaggaggc cggtcccgaa gcgagagggt tcgcgcccgg ggaccgggtg
     2521 ggcgtcccgt ggatgcagtt cgcgtgcggg gagtgcgcac cgtgtctctc cggagccgag
     2581 atgctctgcg cggcgcaaag ggggaccggg gtcaccgtgg acggcggcta cgcggagttc
     2641 gtgaaggcgc ccgcggcgtt cgttcaccgg atcccggaac cgctcgacct cgtcgattcc
     2701 gccccgctcc
//
LOCUS       DASVJL010000206         3880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_687656, whole genome shotgun sequence.
ACCESSION   DASVJL010000206 DASVJL010000000
VERSION     DASVJL010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687656"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VF357_04190"
     CDS             complement(<1..333)
                     /locus_tag="VF357_04190"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX7523377.1"
                     /translation="MGRNAPDGLSLRELTNRIFLSAGLILAGIGIAVATGAANVSLLP
                     GAMCGAAIACGNFFLIRKILEKAFSGGGTVSRGFVVQYTLKFLGLIGVVYLVVRYGGF
                     DLLGFLLGL"
     gene            complement(323..589)
                     /locus_tag="VF357_04195"
     CDS             complement(323..589)
                     /locus_tag="VF357_04195"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX7523378.1"
                     /translation="MTDRQDRKAFFRELAKYSALGLEMALSVVIGMGIGYYLDRWLGT
                     GPWLTVVWIALGFAAGVRSLYRAVVRSGKDLERDEEQRRKPGGP"
     gene            complement(681..1973)
                     /gene="hemL"
                     /locus_tag="VF357_04200"
     CDS             complement(681..1973)
                     /gene="hemL"
                     /locus_tag="VF357_04200"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX7523379.1"
                     /translation="MARLTTNTSSKLFALAKTMIPGGVNSPVRAFGSVGGTPLFIESA
                     KGSTVRDVDGNTFIDYVGSWGPMILGHAPPAVVSAIKAAAGRGTSYGAPTPGEVGLAR
                     LIRKAFPSVEKVRLVSSGTEAVMSAVRLARGYTGRDKIIKFEGGYHGHADSMLVKAGS
                     GVLTFGQPDSPGVPAELAKLTLTAAFNDIASVRALLAKNKRQVAAIFVEPIPGNMGVV
                     PPEPGFLEELRTLAKKHGALLVFDEVISGFRVAFGGAQELFGVVPDLTILGKIIGGGL
                     PVGAFGGRKEIMDALSPVGPVYQAGTLSGNPLAVAAGIAALTELSRRGAYKELNPKAD
                     YLAEGMAKAFAASGVPTWTNRVGSMGTTFFQKGPVTDYASAKRSDTKRYATWFHGMLS
                     RGVYIAPSQFEAGFVSLAHTKRDLDRTIAAAKAVLATL"
     gene            2219..2617
                     /locus_tag="VF357_04205"
     CDS             2219..2617
                     /locus_tag="VF357_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523380.1"
                     /translation="MSVEDKERIETRFGPLWSGKTEIAFRGGVRTLRDVKRALDLTGE
                     DVMAIDLHELPGETFAFRYYDGDDRRIVVLVCDTEGEILEEFRAHIAEWLGELYYESG
                     ALAYDADAMLHLLRKKSEGAGMSPGEADRC"
     gene            2611..2985
                     /locus_tag="VF357_04210"
     CDS             2611..2985
                     /locus_tag="VF357_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523381.1"
                     /translation="MLTCRKTEVRESAAQWHIDALVGAPGGDLFPCFVAVVSSYCTVQ
                     APNTKDGEAIFGDVAGALGAPPSSPPQVVKGGSCGGEEEDGEFPFTGSMISATWEFPK
                     ERRDAVLASFRGLFGLTDGPSR"
     gene            2982..>3880
                     /locus_tag="VF357_04215"
     CDS             2982..>3880
                     /locus_tag="VF357_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523382.1"
                     /translation="MKSYVDLVRQRLEDRGNNIVGNLERFMEPQLRFTMRIFGDCLDE
                     ETRGTMFSGYSEHLTEQELRAFAADFVPAYTRYAIAELEEKKKGGERHEPPFLTQEEY
                     QEMAVREKWPRITEHMDGVTPLQLRREVARAAMLFLPGMLSDPGFNEGVLEFSLYFDL
                     LQRLRSVSETRLRKAAGEIASRIAAAVAATSEEVRKALLREIRTTAATTAGLPADPET
                     LLGPPMEKYPREVPAEFRLRELKNTLATMKLKDLRLSALVHLDLLTVEETRRIVLPFL
                     AKYPSFYEMPSKGLRELILAVAAG"
BASE COUNT          675 a         1288 c         1277 g          640 t
ORIGIN      
        1 gagcccgagc agaaatccca acaggtcgaa cccgccgtac cggacgacga ggtagaccac
       61 cccgatcagc ccgaggaact tcagcgtgta ctggacgacg aaccccctgg agaccgtccc
      121 gccccccgag aacgccttct ccaggatctt ccttataagg aagaagttcc cgcacgcgat
      181 ggcagcgccg cacatcgctc cggggagcaa cgagacgttc gccgctcccg ttgcgaccgc
      241 gatcccgatc cccgcgagga tcagccccgc cgaaaggaag atccggttcg tcagctcccg
      301 caacgacaac ccgtccgggg cgttacggcc caccgggctt cctccgctgc tcctcgtccc
      361 gctccaggtc tttcccggac cgcacgacgg cgcggtagag gctgcgcacc ccggcggcga
      421 acccgagggc gatccagacg accgtcagcc acggcccggt cccgagccac cggtcgagat
      481 agtacccgat ccccatgccg atcacgaccg acagggccat ctcgagcccg agggcgctgt
      541 acttcgccag ttcccggaag aacgcctttc ggtcctgtcg atcggtcatg agggcaaacg
      601 ccggatattt atcacaggat ccgggaacgc gtcaatacaa aaccccggag cggcgggcac
      661 ctattccctt ccttccatgg ttatagggtg gccaggacgg ctttggcggc ggcgatcgtc
      721 cggtcgaggt cccgcttcgt gtgcgccagg gagacgaacc ccgcctcgaa ctgggacggc
      781 gcgatataga ccccgcggga gagcattccg tggaaccagg tcgcgtaccg tttcgtgtcg
      841 ctccgcttcg ccgaggcgta gtccgtcacc gggccctttt gaaagaaggt cgtccccatc
      901 gagcccacgc gattcgtcca ggtcgggacg ccggaggcgg cgaacgcctt cgccatcccc
      961 tccgcgaggt aatccgcctt cgggttcagt tccttgtagg cccccctgcg ggaaagttcc
     1021 gtgagcgcgg cgatccccgc cgccaccgcg agcggatttc cggatagggt ccccgcctgg
     1081 tagaccgggc cgaccggcga gagggcgtcc atgatctcct tccgcccgcc gaaggccccc
     1141 accgggaggc cgccgccgat gatcttgccg agaatcgtga ggtcggggac gacgccgaac
     1201 agttcctgcg cgccgccgaa cgcgacgcgg aacccggaga tcacttcgtc gaagacgagg
     1261 agcgccccgt gcttcttcgc aagcgtccgc agttcctcca ggaagccggg ttccgggggg
     1321 accactccca tgttgccggg aatcggctcc acgaagatcg ccgcgacctg cctcttgttc
     1381 tttgcgagga gcgcccgcac ggaggcgatg tcattgaagg cggcggtcaa ggtgagcttc
     1441 gccagctccg ccgggacgcc gggggagtcg ggctgtccga aggtgagaac ccccgagccg
     1501 gctttcacca gcatcgagtc ggcatggccg tggtacccgc cctcgaactt gatgatcttg
     1561 tcccgccccg tatacccccg cgccagccgc accgcgctca tcaccgcctc ggttccggag
     1621 gagacgaggc ggaccttctc gacggagggg aatgccttgc ggatcaggcg cgccagcccg
     1681 acctcgcccg gcgtgggagc cccgtagctc gttccccgcc ctgcggccgc cttgatggcg
     1741 gagacgaccg cggggggagc gtgtccgagg atcatcggcc cccacgaacc gacgtagtcg
     1801 atgaatgtgt tcccgtccac gtcgcggacg gtggagcctt tcgcgctctc gatgaacagg
     1861 ggggttcccc cgacggaccc gaacgcgcgg acgggggagt tgacaccgcc cgggatcatc
     1921 gttttcgcaa gggcgaagag cttgctcgag gtgttcgtcg tcaggcgggc cacgttcgct
     1981 ccggcctcct tcgagggatg ggattcatgg caagatgtgg aattttagcc ccccaggatt
     2041 ggagtgtcaa ggtaaacagc ggtatttccg gaggatttac cgaattctca aaacaccatt
     2101 atagaaccgc ttgacacggc gggcacccag ggagtacaaa ggtagccttc cccccgcttc
     2161 cgcagcttca tgttttattt ggtgtcgtcc caaggggcgg ggtgaaaggg gtatccgcgt
     2221 gagcgtcgag gacaaggaac ggattgaaac gcgttttggc ccgttgtggt ccgggaagac
     2281 cgagatcgcc ttccgtggcg gggtgcgaac gctccgtgac gtaaaacgcg cgctcgacct
     2341 gacgggcgag gacgtgatgg ccatcgacct gcacgaattg ccgggggaga cgttcgcctt
     2401 ccgctattat gacggcgacg accgccggat cgtggtcctg gtctgcgaca cggaagggga
     2461 gatcctcgag gagttccggg cgcacatcgc ggaatggctg ggggaactct actacgagag
     2521 cggcgccctg gcctacgatg cggatgcgat gctccacctc ctccgaaaaa agagcgaggg
     2581 ggccggaatg tcccccggag aagcggatcg atgctgacct gccggaagac ggaggtgcgg
     2641 gagtccgccg cccagtggca catcgatgcg ctggtggggg cgccgggggg cgacctgttt
     2701 ccctgtttcg tcgccgtcgt ctcgtcgtat tgcaccgttc aggcccccaa cacgaaggat
     2761 ggcgaggcga tcttcgggga cgtggccggg gcgctgggcg ctcccccatc ctctccaccg
     2821 caggtggtca agggcggctc gtgcggcggg gaggaggaag acggggagtt ccccttcacg
     2881 gggtcgatga tctccgccac ctgggaattc ccgaaggagc gcagggacgc cgtcctcgcc
     2941 tccttccgcg ggctcttcgg cctgacggac gggccgtcca gatgaagtcg tatgtcgatc
     3001 tggtgcggca gcgcctcgaa gaccggggga acaacatcgt cgggaacctc gaacgtttca
     3061 tggaaccgca gttgcggttc acgatgcgga tcttcggcga ctgcctcgac gaggagaccc
     3121 gcggcacgat gttctccggg tacagcgagc acctgaccga acaggaactg cgcgcgttcg
     3181 ccgccgattt cgtccccgcg tacacccgat acgcgatcgc cgagcttgag gagaagaaga
     3241 agggcggcga gcgccacgag ccccccttcc tcacccagga ggagtaccag gagatggcgg
     3301 tccgggaaaa gtggccccgg atcacggaac atatggacgg cgtgacgcct ctccagttgc
     3361 gccgcgaggt cgcgcgcgcg gcgatgctgt tccttcccgg catgctctcc gaccccggct
     3421 tcaacgaggg ggtcctcgag ttctccctct atttcgacct tctccagcgg ctgcgctccg
     3481 tgtccgaaac gcggctgcgg aaagcggccg gggagatcgc ctcgcggatc gcggccgccg
     3541 tcgcggcgac ctccgaagag gtacggaagg cgctgctccg ggaaatccgc acgacggcgg
     3601 cgacgacggc gggactgccc gccgacccgg agacgctcct gggtcccccg atggagaagt
     3661 acccgcggga ggtcccggcg gagttccgcc ttcgggagct gaagaacacc ctcgccacga
     3721 tgaagctgaa ggaccttcgc ctctccgcgc tggtccacct cgacctgctg accgtcgagg
     3781 agacccggcg gatcgttctc cctttccttg cgaagtaccc ttccttttat gaaatgccct
     3841 cgaaggggct gcgggagctg atcctggccg tcgccgcggg
//
LOCUS       DASVJL010000207         2643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_593996, whole genome shotgun sequence.
ACCESSION   DASVJL010000207 DASVJL010000000
VERSION     DASVJL010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593996"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /gene="nagZ"
                     /locus_tag="VF357_04220"
     CDS             complement(<1..1122)
                     /gene="nagZ"
                     /locus_tag="VF357_04220"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX7523383.1"
                     /translation="MLKFHSMKHGDTIASPLWVGFDGRSLPASLSRWLAGGQVSGVVL
                     YSRNIESPAQVRGLCREIRSAAGRGNPLPLIAVDQEGGRVARFKDPPFTWFPPARACS
                     LFCCRNEALAEAVGAATAAELRAVGIDVNFAPVLDVDSNPANPVIGDRAFSEDPQAAA
                     SLGIAFAKGSLSRGILPVGKHFPGHGDTSADSHEELPVVRAGRQTLVRRELLPFRRAA
                     RAGFPALMTAHVLYPALDRALPATLSRKILHDLLRKRFRFGGAVISDALEMKAIADRY
                     GLGEAALLAVTAGCDVVLVCKGENEQAETIDRLSREVTDRPAFRRAVAAAAVRSGRLR
                     AWAAAKERCRPAPRAVGAARHRLLSSLLREAWESTGRTSP"
     gene            complement(1116..1973)
                     /locus_tag="VF357_04225"
     CDS             complement(1116..1973)
                     /locus_tag="VF357_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7523384.1"
                     /translation="MSEGSGFLALLRRTARHRGAAVGMVLLVLFLLAAVFAPVIAPGD
                     PLAQNLDEGLSRPSTSHWLGQDKFGRDVLSRLIHGARLSLAVGLGTVGISLLIGLAAG
                     SLAGFLGGAADRIFTGACDVLLAFPGILLAIGIAAVRGPSFGNVLFALSVLGWVGYAR
                     VIRAQVLSVKTRGFVESARAVGSSPARLLLRHILPNAISPILVEATFGIARAIVAEAG
                     LSFLGLGVAPPAASWGSMIGEGRHFLFIAPHLVTAPGAAILLTVMAFNFLGDGLRDAL
                     DVTTTTTPC"
     gene            complement(1966..>2643)
                     /locus_tag="VF357_04230"
     CDS             complement(1966..>2643)
                     /locus_tag="VF357_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7523385.1"
                     /translation="RSREPVLGEIARRIPATVLLAATSITVAVFVALPLGVLAAMRRG
                     KAADYLSGFAAMLSLSIPNFLLGPLLVLLFSVQLGLLPVSGYGAGRPLVLPAVTLGTG
                     MAALLTRMVRATLLEELRKEYVTAATARGLSYRSAVTFHALRNALVPVVTALGLSFGA
                     VLAGSVITETIFSWPGIGRLLIQAIDARDYPLVQGCVLVIALSTVAVNLATDILCARL
                     DPRIRYE"
BASE COUNT          471 a          893 c          956 g          323 t
ORIGIN      
        1 cggagatgtt cgtccggtac tttcccatgc ctcccgcagg agggaagaaa gcagtcgatg
       61 ccgtgccgcc ccaaccgccc gcggcgccgg ccgacaacgt tctttcgccg ccgcccacgc
      121 ccgaagccgt cccgatcgaa ccgccgccgc cgccaccgcc cgccggaacg ccggtcggtc
      181 ggtaacctcc cgggacaggc ggtcgatcgt ctcggcctgt tcgttttccc ccttgcagac
      241 gagcacgacg tcgcatccgg ccgtcactgc gaggagcgcc gcctccccga gcccgtagcg
      301 atccgcgatc gctttcatct ccagcgcgtc ggagatgacc gctccgccaa accgaaaccg
      361 tttccgcaac aggtcgtgaa ggatcttccg ggacagtgtt gccggcaggg cgcggtcgag
      421 cgccgggtac aacacgtggg cggtcatgag cgccgggaac cccgcgcgtg cggcgcggcg
      481 gaaggggagc agttcgcggc gaacgagcgt ttgtcgtccc gcccggacga ccggcagctc
      541 ttcatgggag tcggcggacg tgtcgccgtg gcccgggaaa tgtttcccga ccggcaggat
      601 cccccgggag agcgatccct tcgcaaaggc gatcccgaga gaggccgcgg cctgcgggtc
      661 ttcggagaag gcgcggtccc cgatcaccgg gtttgccggg ttgctgtcca cgtcgaggac
      721 cggggcgaag ttcacgtcga tccccaccgc gcggagctcg gcggcggtcg ccgcgccgac
      781 ggcttccgcc aacgcctcgt tccggcaaca gaagagggag caggctcggg ccgggggaaa
      841 ccaggtaaac ggggggtcct tgaaccgcgc cacgcggcct ccctcctggt cgacagcgat
      901 caatgggagg ggattgccgc ggcccgcggc ggatcggatc tcgcggcaca atccgcgcac
      961 ctgggcgggg ctctcgatgt tgcgggagta gaggacgacc ccgctgacct gcccgcctgc
     1021 gagccatcgc gacagtgagg cgggaagcga cctcccgtcg aagccgaccc acagggggga
     1081 ggcgatggtg tctccatgct tcatggaatg gaactttagc atggggttgt ggttgtggtc
     1141 acgtcgaggg cgtcacggag cccgtccccc aggaagttga acgccatgac ggtgaggagg
     1201 atcgccgccc ccggagcggt cacgaggtgc ggcgcgatga acaggaagtg gcgcccctcg
     1261 ccgatcatcg atccccacga cgccgccggc ggcgcgacgc ccagtccaag gaaagagagc
     1321 cccgcctccg cgacgatggc gcgggcgatc ccgaacgtcg cctccacgag gatgggggag
     1381 atcgcgttgg ggaggatgtg acgcaggagc aggcgcgccg gggagctgcc cacggcgcgg
     1441 gccgactcga cgaaccccct cgttttcacg gagagcacct gcgcgcggat cacccgggcg
     1501 taccccaccc accccaggac cgaaagcgcg aacaggacgt tcccgaacga ggggccgcgt
     1561 acggcggcga tcccgatggc gagcaggatc cccgggaacg ccagcaggac gtcgcaggcg
     1621 ccggtgaaga tccggtcggc ggcgccgccg aggaagccgg cgagggagcc ggcggccaag
     1681 ccgatgagga gcgagatacc gaccgttccc aatcccacgg cgagggagag gcgggctccg
     1741 tggatcagcc gggaaaggac gtcgcgcccg aacttgtcct gccccagcca atgggatgtg
     1801 gaaggccgag acagcccctc gtcgagattt tgtgcaagtg ggtcgcccgg cgcgatcacc
     1861 ggcgcgaaga cggcggcgag gaggaacagg acaaggagga ccatcccgac ggcggcgccc
     1921 cggtgccggg ccgtgcggcg caggagcgcg aggaaaccgg atccttcact catagcggat
     1981 tctcgggtcg agacgtgcgc acagaatgtc cgttgccagg ttcaccgcca cggtggagag
     2041 ggcgatgacg aggacgcacc cttgtaccag cgggtagtcc cgcgcgtcga tcgcctggat
     2101 caggaggcgg ccgatcccgg gccaggagaa gatcgtctcc gtgatgacgc tccccgcaag
     2161 caccgcgccg aacgacagcc ccagcgccgt gacgacgggg acaagcgcgt tgcgcagcgc
     2221 gtggaaggtg acggcggacc ggtacgacag accccgcgcg gtcgcggccg tcacgtactc
     2281 cttccggagc tcctcaagca gcgtcgcccg gaccatccgc gtcagcaggg cggccatccc
     2341 cgtcccgagc gtgacggcgg gcagcaccag cggacgaccc gcgccgtacc ccgacacggg
     2401 gaggagccca agctgcacgg agaagaggag cacgagcagc ggtccgagga gaaagttcgg
     2461 gatggacagg gagagcatcg ccgcgaaccc cgacaggtag tccgcggcct tcccgcgccg
     2521 catcgccgcc agaaccccga gcggaagggc gacgaagacc gccacggtga tggacgttgc
     2581 ggcgagaagg acggtggcgg gaatccggcg ggcgatctcg ccgaggaccg gctcgcggct
     2641 gcg
//
LOCUS       DASVJL010000208         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_126451, whole genome shotgun sequence.
ACCESSION   DASVJL010000208 DASVJL010000000
VERSION     DASVJL010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126451"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /gene="scpB"
                     /locus_tag="VF357_04235"
     CDS             complement(<1..761)
                     /gene="scpB"
                     /locus_tag="VF357_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX7523386.1"
                     /translation="MEETPKPNPPTATDPPASPDPPTMPPAPGEPISVDELLRPASEA
                     ETEPPGPDADLPRAASVLESLLLVSTEPIPIEKARQLLGGLNRAQVREVLALLRAKYP
                     ADSSGILVEEVAKGFQFRTNPANQEHVRRLFDVKPPRFSRAALETLAIIAYKQPLTRQ
                     EIEQIRGVDCAGSLKTLMDRRLVKVMGKKDVPGKPFLFGTSREFMETFSLGSLSELPS
                     MREIEDFLAASTGTPVEEGRPTMHLPAIPDEGIEQG"
     gene            complement(721..1548)
                     /locus_tag="VF357_04240"
     CDS             complement(721..1548)
                     /locus_tag="VF357_04240"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX7523387.1"
                     /translation="MSGSDDKPDLARRDGDASASPVGDVPLRLDVFEGPLDILLHLVR
                     DQKLDINDIPIAKITEQYLAYLDLMQALNLEVAGEFLVMASTLVHIKSKSLLPRHGDE
                     EEEEDPEVMRQELSRRLIEYERFKEAAARLGERPVLGRDVFVRDFLGEEIPGEELVIT
                     ELSMADLITAFKDALSRMPADAAAEFSIERLTIADAIAFLLDRLKDEGSIRFEDIVTH
                     CQSRNEIVSFFLGILELVRLKTIRVYQANPLGLISIVPAVRETENGRDTETESADGD"
     gene            complement(1535..>2497)
                     /gene="trpS"
                     /locus_tag="VF357_04245"
     CDS             complement(1535..>2497)
                     /gene="trpS"
                     /locus_tag="VF357_04245"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX7523388.1"
                     /translation="SGMRPSGQLHLGHYLGVLVNWKKLQEENDCFFFVADWHALTTEY
                     DRTSIIRESIDDMVIDWLASGIDPQKATIFIQSHVPEHAELHLLLSMITPLPWLERNP
                     TYKEQLREQTSRDLQTYGFLGYPVLQAADILMYNATLVPVGIDQVPHLELTREIARRF
                     NFLYKECLTIPEAYLTETSKMMGTDNRKMSKSYGNAILLSDTSEEVWAKVKPMVTDPA
                     RQRRTDCGNPDICNIFSYHKIFSDEATIAKVDVGCRTAGIGCIECKKWMFEGMEKVLA
                     PVREKRLQVVESGVSVRDILADGTRRAREVAGAKMAEVRDAVRI"
BASE COUNT          392 a          794 c          856 g          455 t
ORIGIN      
        1 ccctgctcga ttccctcgtc agggatcgcc ggcagatgca tcgtcggccg gccttcctcg
       61 accggcgtgc cggtggaggc ggcgaggaaa tcctcgatct cccgcatcga cggcagctcc
      121 gagagggaac cgaggctgaa cgtctccatg aactcccgcg aggtgccaaa gaggaacggc
      181 ttgccgggga cgtctttctt ccccatcacc ttcacgagcc ggcggtccat cagggtcttc
      241 aacgacccgg cgcaatccac gcctcggatc tgctcgatct cctgccgggt cagcggctgc
      301 ttgtaggcga tgatcgccag cgtctccagc gcggcgcggg agaagcgcgg cggcttcacg
      361 tcgaagagac ggcgcacgtg ctcctggttc gccgggttcg tgcggaactg gaaccccttt
      421 gccacctctt cgacgaggat ccccgaggag tccgcggggt atttcgcgcg gaggagggcg
      481 agcacctccc gcacctgggc gcggttgagg ccgcccagca gctggcgcgc cttttcgatg
      541 gggatcggct cggtggaaac gaggaggagg ctctccagga ccgaggcggc gcgcggaaga
      601 tcggcgtcgg gccccggcgg ctccgtctcc gcctcggagg cggggcgcag cagctcgtca
      661 acgctgatcg gctccccggg cgccggcggc atcgtcggag ggtcggggga cgccggcgga
      721 tcagtcgccg tcggcggatt cggtttcggt gtctcttcca ttttcggtct cccggacggc
      781 gggtacgatg ctgatcagcc ccagcgggtt cgcctggtac acgcgaatcg tcttgaggcg
      841 gaccagctcg aggatgccca gaaaaaagga tacgatctcg ttcctcgact ggcaatgcgt
      901 tacgatgtcc tcgaaccgga tggagccttc gtccttcagg cgatcgagaa gaaaagcgat
      961 ggcgtcggcg atggtgagcc gctcgatgga gaactccgcg gcggcgtcgg cgggcatccg
     1021 cgacagcgcg tccttgaagg ccgtgatgag gtccgccatc gacagctcgg tgatgacgag
     1081 ctcctcgccc gggatctcct ccccgagaaa atcgcgaacg aagacgtccc gccccagcac
     1141 cggacgctcg cccaggcgcg ccgccgcctc cttgaaccgc tcgtactcga tcagccgccg
     1201 ggacagctcc tggcgcatga cctcggggtc ttcctcctcc tcctcgtccc cgtggcgagg
     1261 cagcagcgac ttcgacttga tgtggacaag ggtggaagcc atcaccagga actcgcccgc
     1321 gacctcgagg ttcagcgcct gcatcaggtc gaggtaggcc aggtactgct cggtgatctt
     1381 cgcgatcggg atgtcgttga tgtcgagctt ctggtcgcgg accaggtgca gcaggatgtc
     1441 gagggggccc tcgaaaacgt cgaggcgcag cgggacgtcc ccgacgggac tcgcggaagc
     1501 gtcgccgtcg cgccgggcga gatcgggctt gtcgtcagat ccggacagcg tcgcggacct
     1561 ccgccatttt ggcgcccgcc acctcccgcg cgcggcgcgt gccgtcggcc aggatgtcgc
     1621 gcacggagac gccgctttcc acgacctgca ggcgcttttc gcggacgggg gcgaggacct
     1681 tttccatccc ttcgaacatc cacttcttgc actcgatgca tccgatgccg gcggtgcggc
     1741 agccgacgtc caccttggcg atcgtcgcct cgtcggaaaa gatcttgtgg taggagaaaa
     1801 tgttgcagat gtccgggttc ccgcagtcgg tgcgccgctg gcgggccggg tcggtgacca
     1861 tcggcttcac cttggcccac acctcttccg acgtgtcgga gaggaggatc gcgttgccgt
     1921 agctcttgct catcttccgg ttgtcggtcc ccatcatctt cgacgtttcg gtcaggtacg
     1981 cctcggggat cgtcaggcac tccttgtaca ggaagttgaa ccgtcgggcg atctcgcggg
     2041 tgagctcgag gtgcgggacc tggtcgatgc cgacggggac gagcgtggcg ttatacatca
     2101 ggatgtccgc cgcctggagg accgggtaac cgaggaagcc gtacgtctgg aggtcgcggg
     2161 aggtctgctc ccggagctgc tccttgtacg tcgggttgcg ctccagccaa ggcagcggcg
     2221 tgatcatgga gaggagcagg tgcagctcgg cgtgctcggg gacgtggctc tggatgaaga
     2281 tcgtcgcctt ttgcgggtcg atcccggagg ccagccagtc gatgaccatg tcgtcgatgc
     2341 tctcgcggat gatggaggtg cggtcgtact cggtggtcaa ggcgtgccag tcggcgacga
     2401 agaagaagca gtcgttctcc tcctggagct tcttccagtt gaccaggacc ccgaggtagt
     2461 gccccaggtg aagctgaccg ctcggccgca tgccgct
//
LOCUS       DASVJL010000209         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1815194, whole genome shotgun sequence.
ACCESSION   DASVJL010000209 DASVJL010000000
VERSION     DASVJL010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815194"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /gene="recO"
                     /locus_tag="VF357_04250"
     CDS             complement(<1..563)
                     /gene="recO"
                     /locus_tag="VF357_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX7523389.1"
                     /translation="MIRPSFHSSPAFLARAIDLGEADRRLTFFTRDAGVLVTVGKSAW
                     RSRKRFGGALQRYVLLDIAWTERPGRMAVLSSASVNRSFWGIVEDWERVRHADHLLEI
                     ASELFPQAGPKPRAFEVLLRGMRSIADGEPPPQTARRAEAEFLAIGGWGPDLSGCRKC
                     KEKEGRSYRFLDSEGGVLCEACSLRAGA"
     gene            complement(560..1426)
                     /locus_tag="VF357_04255"
     CDS             complement(560..1426)
                     /locus_tag="VF357_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX7523390.1"
                     /translation="MTPGPAGSAWKERRESMGKTIDQVSAELRIGNRYLAGIEEGDFG
                     DFPERVFSTGFIRSYAKYLSQDPGPVLVEYERSIGNRDDSEMAAQLRFGWVERDRERG
                     SRRTTYTVAAGAVLLVGVILAWVTLRTERRPLPPPSPPPAVALPSPPAVENAAKTGDN
                     AAKTGDNAAVAAPSGVDNMAEPVLSQTQTAPTPVEPASSVAAVGGTGPLVGPFQLSLE
                     ASEQAWVMYSFDDGDPIDVTLYAGDKISIQAKRRITLKLGNAGGVAGTLNGRRLPPFG
                     ERGQVRKFAFGQ"
     gene            complement(1423..>1816)
                     /locus_tag="VF357_04260"
     CDS             complement(1423..>1816)
                     /locus_tag="VF357_04260"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7523391.1"
                     /translation="AAANVMYTTPERAYFNLGEAYRVKGDPANAEGAYRRALRANEQY
                     APAYIALSAVLGGQGKWEDAASILSRCVVVLPDYAPGWMELGRAYLRLSRPAEAVKAF
                     DRVLAVSSDPEMRKQAAGYVTLLGSEKR"
BASE COUNT          288 a          667 c          587 g          274 t
ORIGIN      
        1 gcccccgccc ggagcgaaca ggcctcgcac aagacccccc cctccgaatc gagaaaccgg
       61 tacgaacgcc cctccttctc cttgcatttc cggcacccgg acaggtcggg cccccacccg
      121 ccgatcgcga ggaattcggc ctcggccctt cgagcggtct gcggcggcgg ctccccgtcc
      181 gcgatggaac gcatcccccg caggaggacc tcgaacgccc ggggcttggg ccccgcctgg
      241 gggaaaagct ccgacgcgat ctcgagcagg tggtccgcgt gtcgcacacg ctcccaatcc
      301 tccacgatcc cccagaaact ccggttcacg gaggcggagg aaaggacggc cattcgcccc
      361 ggacgctccg tccacgcgat gtcgagaagg acgtaccgct gcagcgcgcc gccgaaccgt
      421 ttccggctgc gccacgcgga ttttccgacg gtcacaagaa ctcccgcgtc ccgggtgaag
      481 aacgtgaggc ggcggtcggc ctccccgagg tcgatggcgc gcgcgaggaa agcgggagag
      541 gagtggaagg aagggcggat cactggccga aggcgaactt ccggacctgc ccccgttccc
      601 cgaacggcgg cagccgccgg ccgttcagcg ttcccgccac gccccccgcg ttgccgagtt
      661 tcagggtgat ccgtctcttc gcctggatgc tgatcttgtc gccggcgtac agcgtcacgt
      721 cgatcgggtc gccgtcgtcg aaactgtaca tcacccacgc ctgctcgctc gcctcgaggg
      781 acagctggaa ggggccgacg agggggccgg tgccgcccac cgccgccacc gaagaggccg
      841 gttccacagg tgtcggcgcg gtctgtgtct gggaaaggac cggctccgcc atgttgtcga
      901 cgccggaagg agcggcgacc gccgcgttgt cgcccgtctt cgccgcgttg tcgcccgtct
      961 tcgccgcgtt ctccaccgcc ggtggggaag gaagcgcgac agcgggaggc ggcgacggcg
     1021 ggggcaacgg acggcgctcg gtgcggaggg tcacccaggc gaggatcacc ccgacgagga
     1081 gaacggcacc ggcggcaacg gtgtacgtcg tccggcggct cccccgctcc cggtcccgct
     1141 ccacccaccc gaatcgcagc tgcgccgcca tttcactgtc gtcacggttc ccgatcgacc
     1201 gctcgtactc gacgaggacg ggccccggat cctgcgagag gtatttggcg tacgagcgga
     1261 tgaagccggt ggagaatacg cgctccggga aatccccgaa gtccccctcc tcgattcccg
     1321 cgagataccg attgccgatc cggagctcgg cggaaacctg gtcgatcgtc ttccccattg
     1381 actcccggcg ttccttccag gcggaaccag cgggccccgg cgtcatcgct tctccgatcc
     1441 gaggagcgtc acataaccgg ctgcctgttt cctcatttcg gggtcgctcg aaaccgcgag
     1501 aaccctgtcg aacgccttca ccgcctccgc cggtcgcgag agccggaggt aggcgcgccc
     1561 cagttccatc cagcccgggg cgtagtcggg aaggaccacc acgcaccggc tgaggatgga
     1621 cgccgcatcc tcccacttcc cctgcccgcc gaggacggcg gaaagcgcta tataggcggg
     1681 ggcgtactgc tcgttcgccc gcagcgcccg ccggtacgcc ccttctgcgt tcgccggatc
     1741 ccccttgacg cgatacgcct ccccgaggtt gaaatacgcc cgttccggag tcgtgtacat
     1801 cacgttcgcg gcggcc
//
LOCUS       DASVJL010000210         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_407356, whole genome shotgun sequence.
ACCESSION   DASVJL010000210 DASVJL010000000
VERSION     DASVJL010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407356"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..657)
                     /locus_tag="VF357_04265"
     CDS             complement(145..657)
                     /locus_tag="VF357_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX7523392.1"
                     /translation="MLVLWLFGGMLAFSPLLDSPASADIYRWEDESGVIHFTDDLSAV
                     PAKYRKKAREIQKTPPEAGKPSVSTMGAPSSPPGPSSSPRPSNGETLNRPERTQDDDA
                     TLAENLRAKIDAKDRFLRAVDEKQSLATNPLRNRIVSPSDLELYRKYKEELPGDRERL
                     KALESRLPHK"
     gene            740..928
                     /locus_tag="VF357_04270"
     CDS             740..928
                     /locus_tag="VF357_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523393.1"
                     /translation="MGGKAAVKAWILLFLAIFSWPFLGVLDLPTTFLGVPSFPAAVFL
                     FWGVLVYALYAASRRAGE"
     gene            944..>2014
                     /locus_tag="VF357_04275"
     CDS             944..>2014
                     /locus_tag="VF357_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523394.1"
                     /translation="MPETLFDGSFVLAVVTGYLLLLFAIGYLGERKFDALRARGLDRY
                     VYVFSAGVYCTSWTFYGCIGNAAQYGPSFLALFIGPSVFALSWWTVLRKLVRFCRTHN
                     VTSVPDLLSVRYGGTPWLGALATLFLVIGSVPYISLQLRAVAISYDTIVGSPGGGLAA
                     AVDPMLPVTLFLAAFALFFGGRYLDFTRPQGGVLTAVATESVVKLVILLAAGAFVCYG
                     VFDGVGEIYSRALAHPEWKKLTGLSDPAGNGYARWTAYFAIAVIDVFLLPRQFHVFVV
                     QNGDESHIKTAMWMFPLYLLLINLFIIPIAFGGLVLGLPAAAGDKFILAIPMLAGNRA
                     MSLIVFLGGFSAATAMVVVSSVA"
BASE COUNT          274 a          660 c          658 g          422 t
ORIGIN      
        1 cccggagtgc ttcaattcat aaaaacggta atacgatcgt gggaactcag actccgggct
       61 cgtgcccccc gttattcctg ctgcgggggt accccaccag cgcgaagctc gtagtggggc
      121 gggggggctt cctctccgct aaggctactt gtgggggagg cgggactcga gcgccttcag
      181 gcgctcccgg tcgccgggaa gctcttcctt gtacttccgg tacagctcga ggtccgaggg
      241 ggagacaatc cggttccgta acgggttggt cgcgagggac tgcttctcat ccacggcgcg
      301 aaggaaccgg tccttcgcgt cgatcttcgc gcgaagattt tccgccagcg tcgcgtcatc
      361 gtcctgcgta cgctctgggc gattgagcgt ctctccgttc gacggcctcg gggaggagga
      421 cgggcccggc ggcgaggagg gggcgcccat cgtggagacg gacggctttc cggcctcggg
      481 cggtgttttc tggatctcgc gggccttctt cctgtatttt gcggggacgg ccgagaggtc
      541 gtcggtgaag tggatcacgc cgctttcgtc ctcccaccgg tagatgtcgg cggaggcggg
      601 agagtcgagg agcggagaga aggcaagcat ccccccgaac agccaaagaa ccagcatcgc
      661 cgctccagaa ggacgtttct tccgattcat cgtctgatag tacaataagt gatccatcat
      721 gggcaacgga ggggttcgat tgggcgggaa agcggccgtg aaggcgtgga tcctcctgtt
      781 cctcgccatt ttctcgtggc ccttcctcgg cgtcctcgac cttcccacga ccttcctcgg
      841 cgtcccgtcg tttccagccg cggtcttcct cttctggggg gtcctcgtgt acgccctcta
      901 cgccgccagc cgccgcgcgg gggagtaggc gagtgaagcc ctgatgccgg aaacgctgtt
      961 cgatgggagc ttcgtccttg ccgtcgtgac ggggtacctg ctcctgctgt tcgcgatcgg
     1021 gtacctcggg gagaggaagt tcgacgccct gcgcgcgcgc gggctcgacc ggtacgtgta
     1081 tgttttttcc gccggtgtct actgcacctc ctggacgttc tacggctgca tcgggaacgc
     1141 cgcgcagtac ggcccttcct tcctcgccct cttcatcggc ccatcggtct tcgccctgtc
     1201 gtggtggacg gtgttgcgga agctggtccg gttctgccgc acccacaacg tgacgtcggt
     1261 ccccgacctc ctgagcgtgc ggtacggcgg gactccgtgg ctgggggcgc tcgcgaccct
     1321 cttcctcgtg atcggcagcg tgccgtacat ctccctgcag ctgcgcgccg tcgccatctc
     1381 ctacgacacg atcgtcgggt cccccggagg cggcctggcc gccgcggtcg acccgatgct
     1441 ccccgtcaca ctgttcctcg ccgcgttcgc cctcttcttc ggtgggcgct atctcgactt
     1501 cacccgcccg cagggaggcg tcctcacggc ggtggcgacc gaatccgtgg tcaagctcgt
     1561 gatcctcctc gcggcgggag cattcgtctg ctacggggtg ttcgacgggg tgggggagat
     1621 ctactcccgg gccctggccc acccggaatg gaagaaactg acggggttgt cggacccggc
     1681 ggggaacggc tacgcccggt ggacggccta tttcgccatc gccgtgatcg acgtattcct
     1741 gctgccgcgc cagttccacg tgttcgtcgt gcagaacggc gacgagtcgc acatcaagac
     1801 ggcgatgtgg atgttcccgt tgtacctgct cctcatcaac ctcttcatca tcccgatcgc
     1861 gttcgggggg ctggtcctgg gccttcccgc cgccgcgggc gacaagttca tcctcgccat
     1921 cccgatgctc gcggggaacc gggcgatgag cctgatcgtc ttcctcggcg gattttccgc
     1981 cgccacggcg atggtcgtcg tctcctcggt ggcg
//
LOCUS       DASVJL010000211         5611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1626183, whole genome shotgun sequence.
ACCESSION   DASVJL010000211 DASVJL010000000
VERSION     DASVJL010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626183"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VF357_04280"
     CDS             complement(<1..723)
                     /locus_tag="VF357_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEX7523395.1"
                     /translation="MAGLDFSELLRRYREALGQAQSSQGKTIPGGGYMGPKPGLIKVV
                     LVVIVVFLVFSGSLVIIGPGQRGVVINFGAVSDQVWDEGLHFKVPIYQRVEKMDVRVQ
                     KEQTDAEAASKDLQDTRSTIAVNFNIIPDKAGWVFQHIGRAYNERVIDPVTQEVVKAV
                     TARYTAVELITNREKIRTEIKALLKTRLLDYHISVVDVSIVNFKFSAQFAQAIENKQT
                     AEQMALKASRDLDRIKIEAQQKI"
     gene            1081..2337
                     /locus_tag="VF357_04285"
     CDS             1081..2337
                     /locus_tag="VF357_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7523396.1"
                     /translation="MGHIRNGNGVYGRLQKRLDRFPIGAPPASALYEILKRLYTEEEA
                     EIAARMPIRFTDLAGIARRTKKSEDVLRPILHRMADKGLVMDFEHRGKFTYILSPTVI
                     GFFEFAFMRVRSDLPQKEIAEHMVAYVHDEPDFARAVFAGKTQAGRTLIHEDAVDPAD
                     RTQILDHERATHIVKEAKAWAVSLCYCRHIQEHEGKACGKPTEVCTTLNSAADYVIRH
                     GLGRRISREEALDIFARTRTEGLVHIGDNVRRRPAYVCHCCGCCCAMLSAINRFKMFD
                     AVVTSPFEASFDPAKCNGCGVCAKKCPVSAIHIDGDEKGKKAALNPGICLGCGVCKPT
                     CGRGALSMAARKERVLVPETAWQRAVVMAIERGKFQNLLFDDFDRLDHAALRAVTRIV
                     VGLPPVKKALLSQQVQSRFFRALAGG"
     gene            2387..3091
                     /locus_tag="VF357_04290"
     CDS             2387..3091
                     /locus_tag="VF357_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7523397.1"
                     /translation="MKTDEFGPGETWRVFRIMSEFVEGFETLKDLGPAITIFGSARTT
                     RGEGSYKATLKVAKMLAQRGFAIISGGGPGIMEAANRGARMGKGVSVGLNIKLPVEQK
                     PNGYQDVSLTFRHFFARKVMFVKYASGYVIMPGGFGTLDEFFESLTLLQTGKIRRFPV
                     VLMGRKYWEGLIRWMEQTLVEEGTISAADLNLFYLADHPEDAVEYIVKFQREMIRPTG
                     ERRKPSPVPSPVSQHE"
     gene            complement(3147..3950)
                     /locus_tag="VF357_04295"
     CDS             complement(3147..3950)
                     /locus_tag="VF357_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7523398.1"
                     /translation="MPYTDAPRFRMYYEEHGSGFPLLLINGLGSDHLEWLHQLPAFEA
                     RFRVLVFDNRGTGMPDVPPGPYSSTQMADDAASLLRALGIPRAHVLGVSLGGMIAQEV
                     ALRHPDLVEGLVLGCTGPGGELSIRPSPEAMAAFALAKGEDPEAKLRRMIPFLYSDAC
                     IRERPEEIEMFVRRRLDHPTPPEGYLAQLFAAVSHDASSRLEKIRAKTLVITGDADRL
                     VNWENSLRLAGRIPGATLVVLPGAPHRLFAETADAFNREVLRFLHHGVV"
     gene            3998..>5611
                     /locus_tag="VF357_04300"
     CDS             3998..>5611
                     /locus_tag="VF357_04300"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HEX7523399.1"
                     /translation="MDAFLLKQVIAELAVDIPGALVSKVYQPGEKEIILDLWGRGEKR
                     LLLSADPELCRIHLTTRKTPNPPSPPRFCQFLRKHLEGMRIAGFSVAPYDRSVRIDFV
                     SSRPDAEHAKTSLYAELFGRHANLIYVDSDGTILEPLRTVSDEESRIREVVPGIPYRP
                     LPKPARVFLPDVTRDDAARIFGSGWKGIPKALQENVAGIGREVAHEAATRGQESPDAL
                     YKVLRDLVRRYEESDFSPGIGTLSDGKRRILPFPCPAAGFADFFPFPTANAAADAFYA
                     EVAQAREIAVLRQQVKSRIAALLKKERHKLENVGGDEERLVEGLQGTARGETLKENLG
                     ALRKGMDSFRNIPLDPAKTPVENMNRFFRLARKAKGATEIVRKRKREVAESVYYLESL
                     EDQLEAARMRDEVIAVRQELSSSFPSRVKPSAKKKSARKDVPRPILPQVEKVEFRGYA
                     ILVGRNNVGNDRIVKELSAPDDLWLHAQGIPGSHVLVKRLAGKEVPREVIEEAARLAV
                     YHSKAKGSRNVPVFLAEARHVSKFKGAKPG"
BASE COUNT         1066 a         1773 c         1844 g          928 t
ORIGIN      
        1 gatcttctgc tgcgcctcga tcttgatccg gtcgaggtcc cggctcgcct tgagcgccat
       61 ctgctcggcg gtctgcttgt tctcgatcgc ctgggcgaac tgggcgctga acttgaagtt
      121 gacgatggag acgtccacca ccgagatgtg gtaatccagc agccgcgtct tcagcagggc
      181 cttgatctcc gtgcggatct tctcccggtt cgtgatcagc tcgacggccg tgtaccgtgc
      241 cgtgaccgcc ttcacgacct cctgggtcac cgggtcgatg acacgctcgt tgtacgcgcg
      301 cccgatatgc tggaagaccc atcccgcctt gtcggggatg atattgaagt tgaccgcgat
      361 cgtggagcgg gtgtcctgca ggtccttgga ggccgcctcc gcatccgtct gctccttctg
      421 gacccgcacg tccatcttct cgaccctttg gtagatgggg accttgaagt ggagaccttc
      481 gtcccacacc tggtcggaaa ccgccccgaa gttgatgacg accccgcgct ggccgggccc
      541 gatgatcacg agcgatccgg agaaaaccag gaagacgacg atcacgacca ggacgacctt
      601 gatcaggccg ggtttcggac ccatgtaccc acctcccggg atggtctttc cttgagaaga
      661 ttgcgcctgt ccgagcgctt ccctgtagcg gcgaagaagc tcggagaaat cgaggcctgc
      721 catgcagaca ctcccttccg cgacgccgac cggtccatcg gaccgtcggt gccgccgttc
      781 cccgaaactg taccttagga ggctagccga aacaacttga acatcttgca tcccgtcttc
      841 gggccatctt tgaccggtct tcaatcgcca aatcctcgac gcagctttaa ctacgcctgc
      901 ggtttggctc aatcatcccg gccaaatcta acccaaatcc gggcgcaata ttgtccaact
      961 tgtttcggct agcctcctta acgtccctgt gctgtccaat gggtcatctg ctatcctgtg
     1021 ggggccggaa cggaggcctg gcatcgcagg gggcgcaacg gttttcggca gggaggaacc
     1081 atggggcaca tccgcaacgg gaacggggtg tacggacggc tgcagaagcg gctggaccgg
     1141 ttccccatcg gcgcgccgcc cgcatcggcg ctctacgaga tcctgaaacg cctctacacc
     1201 gaggaggaag cggagatcgc cgcccgcatg ccgatccggt tcacggacct ggccggcatc
     1261 gcccgacgca cgaagaaatc cgaggacgtg ttgcggccga tcctccaccg gatggcggac
     1321 aagggcctgg tgatggactt cgagcaccgg gggaaattca cctacatcct ctcccccacc
     1381 gtgatcggct tcttcgagtt cgccttcatg cgggttcgaa gcgacctgcc gcagaaggag
     1441 atcgcggagc acatggtggc gtatgtccat gacgaacccg acttcgcgcg cgccgtcttc
     1501 gccggaaaaa cgcaggcggg tcggacgctc atccacgaag acgcggtgga tccggcggac
     1561 cggacgcaga tcctcgacca cgaacgggcg acccacatcg tgaaggaggc gaaggcgtgg
     1621 gcggtctccc tgtgctactg ccgccacatc caggagcacg aggggaaggc atgcggaaaa
     1681 ccgacggagg tgtgcaccac gttgaattcc gcggcggact atgtgatccg ccacggactg
     1741 gggcgacgga tctccaggga ggaggcgctc gacatcttcg cgcggactcg cacggaaggg
     1801 ctggtgcaca tcggggacaa cgtgaggcgc cgtcccgcgt atgtctgcca ctgctgcggc
     1861 tgctgctgcg ccatgctttc cgccatcaac cggttcaaga tgttcgacgc cgtcgtcacc
     1921 tcgcctttcg aggcgtcatt cgatccggcc aaatgcaacg ggtgcggggt gtgcgcgaaa
     1981 aagtgccccg tgtccgcgat acatatcgat ggggacgaaa agggaaagaa agcggccctg
     2041 aatcccggga tctgcctcgg gtgcggggtc tgcaaaccaa cgtgcgggcg gggggcgctc
     2101 tccatggcgg cgcgcaagga acgtgtactc gtcccggaga ccgcctggca gcgcgcggtg
     2161 gtgatggcga tcgagcgggg gaagttccag aacctgctgt tcgacgactt tgaccggctc
     2221 gaccacgccg ccctccgcgc ggtgacgcgg atcgtcgtcg gcctcccccc ggtgaaaaaa
     2281 gcgcttctgt cgcagcaggt gcagtcgcgc ttcttccggg ccctcgcggg cggctgaaga
     2341 ggtaccaacg gatagcgcgg gattccagga acaaacagga ggaacgatga agacggacga
     2401 gttcggtccc ggcgaaacgt ggcgcgtctt ccggatcatg agcgaattcg tggaggggtt
     2461 cgaaacgctc aaggacctgg gcccggcgat aacgatcttc ggcagcgcac ggacgacaag
     2521 gggcgaggga tcgtacaagg ccaccctcaa agtcgcgaaa atgttggcac agcgagggtt
     2581 cgccatcatc tccggcggcg gcccgggaat catggaggcg gcgaaccggg gagcgcggat
     2641 gggcaagggc gtctcggtgg ggctcaacat caagctcccc gtggaacaga agccgaacgg
     2701 gtatcaggac gtgtcgctta ccttccggca cttcttcgcc cgcaaggtga tgttcgtgaa
     2761 atacgcttcc gggtacgtca tcatgccggg gggattcgga acgctggacg aattcttcga
     2821 gtcgctgacc ctcctccaga cgggaaagat ccgccgattc cccgtcgtgc tgatggggcg
     2881 gaaatattgg gagggtctga tccgctggat ggagcagacg ctggtcgaag aggggacgat
     2941 ctccgcggca gacctgaacc tgttctacct ggcggatcac cccgaagatg cggtggagta
     3001 catcgtcaag ttccaacgcg aaatgatccg gcccacgggg gagcgcagga agccgagccc
     3061 ggtgccgtcc ccggtatcgc agcacgaata gtccgggggg aacacgggcg gaaaggaaga
     3121 aacaccgtag cgaagaagat cgtcgtctac accaccccgt ggtgcaggaa ccggaggact
     3181 tcccggttga aggcgtcggc ggtctcggcg aagaggcgat ggggggcgcc gggcagcacg
     3241 accagcgtcg ctcccggaat ccgtcccgcg agccgaaggg aattctccca attcacgagg
     3301 cggtccgcgt ctcccgtgat gacgagcgtt ttcgcccgga tcttctcgag ccgcgatgac
     3361 gcgtcgtggg acaccgcggc gaagagttgg gcaaggtacc cctccggcgg cgtcgggtgg
     3421 tcaagtcgcc tccggacgaa catctcgatc tcctcgggcc gctcacggat gcacgcgtcg
     3481 gagtagagaa acgggatcat ccggcgcaac ttcgcttccg ggtcctcccc cttcgcaagg
     3541 gcgaacgccg ccatcgcctc cggggacggc cgaatcgaga gctcccctcc cggcccggtg
     3601 caccccagca ccaacccctc caccagatcc ggatgccgca gcgcaacctc ctgcgcgatc
     3661 atccctccca gggaaacgcc gaggacatgg gcccggggga tgccgagagc ccgcagcagc
     3721 gacgccgcgt cgtccgccat ctgcgtcgac gaatacggcc cgggcggaac gtcgggcatc
     3781 ccggttccac ggttatcgaa aacgagcacc cggaagcgcg cctcgaaggc gggaagctgg
     3841 tggagccact cgaggtgatc gctccccaac ccgttgatca gcagcagggg gaaaccgctc
     3901 ccgtgctctt cgtaatacat ccggaaccgg ggagcgtcgg tgtacggcat gtcggaggtc
     3961 ctcccttcga cattatagcc gccttataat ggggtcgatg gacgcctttc tcctcaaaca
     4021 ggtgatcgcg gagctcgcgg tggacattcc cggagcgttg gtctcgaagg tttaccagcc
     4081 gggggagaag gagatcatcc tcgacctgtg gggccggggg gagaagcggc tgctcctgtc
     4141 cgccgacccg gagctgtgcc gcatccacct aacgacgcgc aagaccccga acccgccgtc
     4201 gccgccccgc ttctgccagt tcctccggaa gcaccttgag gggatgcgga tcgcggggtt
     4261 ttccgtcgcc ccgtacgatc gctccgtgcg gatcgacttt gtctcgagcc gcccggacgc
     4321 ggagcacgcg aagacatccc tgtacgccga gctgttcggc cgccacgcca acctgatcta
     4381 cgtggacagc gacgggacga tcctcgaacc gctgcgtacg gtatccgacg aggagagccg
     4441 gatccgggaa gtcgtccccg ggatcccgta tcgtccgttg ccaaagcccg cgcgggtctt
     4501 cctgcccgat gtgacgcggg acgacgccgc gcggatcttc gggagcgggt ggaagggaat
     4561 cccgaaggcg ctgcaggaaa acgtggctgg catcgggcgg gaagtcgcgc acgaggcggc
     4621 gacgcgggga caggaaagcc ccgatgcgct gtacaaagtc ttgcgcgacc ttgtccgccg
     4681 gtacgaggag agcgacttct cccccgggat cgggacactc tccgacggga agcggcggat
     4741 cctccccttc ccttgcccgg cggcggggtt cgccgacttc tttcctttcc cgaccgccaa
     4801 cgcggcggcc gacgcttttt acgcggaagt cgcacaggcg cgggaaatcg ccgtcctccg
     4861 acagcaggtc aaatcgcgga tcgccgcgct cctgaagaag gagcgccaca agctggaaaa
     4921 tgtcggaggc gacgaggaac ggctcgtcga ggggctgcag gggaccgcgc gcggagagac
     4981 gctgaaggag aacctcgggg cgctgaggaa ggggatggat tccttccgga acatcccgct
     5041 cgaccccgca aagacccccg tggagaacat gaaccggttc ttccgcctcg cccggaaggc
     5101 gaagggggcg acggagatcg ttcggaagcg gaagcgcgag gtggccgaat ccgtctacta
     5161 cctcgagtcg ctcgaggacc agcttgaggc cgcgcggatg cgggacgaag tgatcgccgt
     5221 gcggcaggaa ctctcttcct ccttcccgtc gagggtaaaa ccgtccgcga aaaagaagag
     5281 cgctcgaaag gacgtgcccc gtcccatcct cccgcaggtg gagaaggtcg agtttcgcgg
     5341 ctacgcgatc ctggtggggc gcaacaacgt cgggaacgac aggatcgtca aggagctctc
     5401 cgcccccgac gacctgtggc tccacgcgca ggggatcccg ggaagccatg tgctggtgaa
     5461 gcgcctcgcc gggaaagagg ttccccgcga agtgatcgag gaggcggcac ggctggcggt
     5521 ttaccacagc aaagcgaagg ggtcgcgcaa cgtcccggtc ttcctcgcgg aggcccgtca
     5581 cgtctccaag ttcaaggggg ccaaaccggg c
//
LOCUS       DASVJL010000212         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_502226, whole genome shotgun sequence.
ACCESSION   DASVJL010000212 DASVJL010000000
VERSION     DASVJL010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502226"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VF357_04305"
     CDS             <1..913
                     /locus_tag="VF357_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7523400.1"
                     /translation="VASFMLINAILGVSIYVTLSTGQLSLGNAGFMGIGAYTAALLST
                     QHQVPIPVGILAGSLLAGAAGILVGIPSLRLSGVYLAIATLGFGEVLRAVLINWESLT
                     GGAVGIAAIPQMGRVILAWASDRGFSPGSLGLQNNQFISLTVFLILLLCTILTVAFFL
                     RLTRSRVGRAYAAIRLDESAAAAAGIPITYYKVLAFSQGALLSGLAGALFAHSTSFVS
                     PADFTYHRAVEILVFAVFGGSEQIFGPVFGAAFLTVVPEALRSISDYRYILYGLLLVL
                     MMIFRPQGVIDPALVRRFRRTGKGSPA"
     gene            910..1692
                     /locus_tag="VF357_04310"
     CDS             910..1692
                     /locus_tag="VF357_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523401.1"
                     /translation="MSSLLSLRKIGKSFGGLSALSDISFDVPSGEIVGIIGPNGAGKT
                     TLFNVVTAVFPPTSGEVVFDGERISGFPSHAITKRGVTRTFQNIRLFSTMTVEENVMV
                     GRHCRTGAGVWRGVLRTRGERREEQGIRGKTRELLDLVGLSGADRDRTAGSLPYGHQR
                     RLEIARALAAEPRLLLLDEPVAGMNDAETQEVFGLIKRVQSLGVTILLIEHDMSLVMK
                     ACDRLVVFNFGRKIAEGTPAEIRDDPLVIEAYLGAAEGNADA"
     gene            1685..2392
                     /locus_tag="VF357_04315"
     CDS             1685..2392
                     /locus_tag="VF357_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523402.1"
                     /translation="MLEARGIETYYGNIPALRGISIDVPVGSVVAIIGTNGAGKTTLM
                     KTIAGVLRPRSGTVSFLGEEITGLPSHKIVRRGVALVPEGRAILSRMTVRENLEMGAF
                     TRRDAKKAVQDMDRVMERFPVLKARAGQLGGSLSGGEQQMLAIARALMSAPKLLLLDE
                     PTLGLAPLVVTDIFAIIREINATGTTILLVEQNVKQALKVSSYTYVLETGKIVLSGPS
                     KELLQEPRIKASYLGQG"
     gene            complement(2364..>2701)
                     /locus_tag="VF357_04320"
     CDS             complement(2364..>2701)
                     /locus_tag="VF357_04320"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7523403.1"
                     /translation="YAGLRDATGFYAMAACVAVVLGGSQALSRSLFSRMIPFGQEAEY
                     FSMYEVSERGTSWLGPLFFGLALQFTGSYRVAILSLAVFFIAGLALLLRVDVARAEAE
                     ATLGRGRTP"
BASE COUNT          434 a          917 c          895 g          455 t
ORIGIN      
        1 ggtggcgtcg ttcatgctca tcaacgccat cctcggcgtg tccatctacg tcaccctttc
       61 caccgggcag ctctcgcttg ggaacgccgg gttcatgggc atcggagcgt acaccgccgc
      121 ccttctctcc acgcagcacc aagtcccgat ccccgtgggg atccttgccg ggagcctcct
      181 cgccggcgcg gcggggatcc tggtcggcat accgtccctg cggctctccg gcgtctacct
      241 cgccatcgct acgctcggat tcggggaggt cctccgggcc gttctcatca actgggaatc
      301 gctgaccggc ggcgcggtgg ggatcgcggc gatcccgcag atggggcgcg tgatcctcgc
      361 ctgggcgagc gaccgggggt tctcccccgg atcgctgggg ctgcaaaaca accagttcat
      421 cagccttacg gtgttcctga tccttctcct ctgcacgatc ctgacggtcg ccttcttcct
      481 gcgcctcacc cgctcccggg tcggccgcgc gtacgccgcc atccggctgg acgaaagcgc
      541 cgcggcggcg gcggggattc ccatcacgta ctacaaggta ctggcttttt cccagggagc
      601 gctgctgtcc gggttggccg gggcgctgtt cgcccactcg acgtcgttcg tgagccctgc
      661 cgacttcacc taccaccggg cggtggagat tctcgttttc gccgtcttcg gcgggagcga
      721 gcagatcttc ggtcccgtct tcggcgccgc cttcctcacc gtcgtccccg aagctcttcg
      781 ctcgatcagc gactaccggt acatcctcta cggcctcctg ctcgttttga tgatgatctt
      841 ccggccgcag ggggtgatcg accccgcact ggtgcgacga ttccgccgaa ccggaaaggg
      901 gtcgcccgcg tgagcagcct cctttccctc cggaagatcg gaaaatcgtt cggggggcta
      961 tcggccctct ccgacatctc cttcgacgtc ccctcggggg agatcgtcgg catcatcggc
     1021 ccgaacggcg ccggaaagac gacactcttc aacgtggtca ccgccgtctt cccgccgacc
     1081 tcgggggaag tcgtgttcga cggggagcgg atcagcgggt tcccttctca cgcgatcacg
     1141 aagcgtggcg tcactcggac gttccagaac atccgcctct tctccaccat gaccgtcgag
     1201 gaaaacgtca tggtagggag acattgccgc accggggccg gcgtttggag aggcgtgctg
     1261 cgcacccggg gggagcggcg ggaggaacag gggatccgcg ggaaaacacg ggagctgctc
     1321 gacctcgtcg gactttccgg ggccgaccgt gaccggaccg cggggagcct cccctacggc
     1381 caccagcggc ggctcgagat cgcccgggcg ctggcggcgg agccgcgcct gctcctgctc
     1441 gacgagccgg tcgcggggat gaacgacgcg gagacgcagg aggtgttcgg gctcatcaag
     1501 cgggtgcagt cgctgggcgt tacgatcctg ctgatcgagc acgacatgtc gcttgtgatg
     1561 aaggcatgcg accggctggt ggtgttcaac ttcggccgga agatcgcgga agggacgccg
     1621 gcggagatcc gggacgaccc gctggtgatc gaggcgtacc tcggcgccgc ggaagggaac
     1681 gccgatgctt gaggcccggg gcatcgagac gtattacggc aacatccccg ccttgagggg
     1741 gatctccatc gacgtccccg tgggaagcgt cgtcgccatc atcgggacga acggggcggg
     1801 aaagaccacg ctgatgaaga cgatcgcggg ggtccttcga ccgcgctccg gcacggtttc
     1861 cttcctcgga gaggagatca ccggcctccc ctcccacaag atcgtgcggc gcggcgtcgc
     1921 gctggtcccg gaggggcggg cgattctctc caggatgacg gtccgcgaga acctcgagat
     1981 gggggcgttc acgcggcgcg acgcgaagaa ggcggtgcag gacatggatc gcgtgatgga
     2041 gcggttcccc gtcctcaagg cacgggcagg gcagcttggc gggtcgctct cgggcggcga
     2101 gcagcagatg ctggcgatcg ctcgcgcgct gatgtccgcg ccgaaactcc tgctgctgga
     2161 cgagcccacc ctcggtctcg cgccgctcgt ggtgaccgac atcttcgcca tcatccgcga
     2221 gatcaacgcg accgggacca ccatcctgct cgtcgagcag aacgtgaagc aggcgctgaa
     2281 ggtctcttcc tacacctacg tcctcgagac gggcaagatc gtcctctccg gcccctccaa
     2341 agagctgctt caggagccgc ggatcaaggc gtcctacctc ggccaagggt agcctccgcc
     2401 tcggcgcgcg cgacgtccac gcgcagcagg agggcgagtc ccgcgatgaa gaagaccgca
     2461 agcgagagga tcgcgacgcg gtagcttccg gtgaactgca gcgccagccc gaaaaagagg
     2521 ggccccagcc agctcgtccc ccgctcgctc acctcgtaca tggagaagta ctccgcctcc
     2581 tggccgaacg ggatcatccg cgagaagagg gaccgggaaa gggcctggct ccctccgagg
     2641 acgacggcca cgcacgccgc catcgcgtag aacccggtcg catcccgaag cccggcgtat
     2701 g
//
LOCUS       DASVJL010000213         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_595187, whole genome shotgun sequence.
ACCESSION   DASVJL010000213 DASVJL010000000
VERSION     DASVJL010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595187"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1538)
                     /gene="pheT"
                     /locus_tag="VF357_04325"
     CDS             complement(<1..>1538)
                     /gene="pheT"
                     /locus_tag="VF357_04325"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7523404.1"
                     /translation="DTAPGKPLADALGLSDWLLEVEITPNRGDCLSVLGVAREIASIT
                     GEKVVLPDVSFPEEGEPIGELVRIDVSDPDLCTRYTARAISGVTIAPSPDWLRRRLTL
                     CGIRPINNIVDVTNYLLLEVGQPMHAFDLDRLRGARIDVRAPKEMLSFTTLDGSEREI
                     DPGMLLILDGEGPVAVAGVMGGANSEVVDGTTRVVFESAHFLPSSIRRTAKRLGLSSE
                     ASYRFERGVDPAGTVYAADRAVSLLSRFAPLSVAKGVIDVGGENVKPRTVPFRPERAT
                     RIMGKVYAPEACMEIFGRLGFPIEDNGTGMWNVTVPTHRFDIEREIDLVEEVARLSGY
                     DSIPTTYPESKAPEFSGDDRFVDVQERAFDFLRGRGFSQAVNFSFVAGRTWERLGAFL
                     GFDPADAIRLVNPISDDTTLMRPHLLTGLLSNVADNVRRFIDDVRLYEGGKAFGKSYV
                     EGHFEEARLGMILCGKRLPGDWSGADTPADFFDMKGVVHPLLLQLCASPVHVVSTRLR
                     PFFE"
BASE COUNT          277 a          522 c          490 g          249 t
ORIGIN      
        1 tcctcgaaaa acggccggag gcgcgtggag acgacgtgaa ccggggatgc gcaaagttgc
       61 agcagcagcg gatgcacgac ccctttcatg tcgaagaagt ccgcgggagt gtccgcaccg
      121 gaccagtcgc ccggaagccg cttcccgcag aggatcatcc cgagccgggc ctcctcgaag
      181 tgcccctcga cgtacgactt cccgaacgcc tttcccccct cgtagagccg gacgtcgtcg
      241 atgaaacgac gtacgttgtc cgccacgttc gacaggagcc cggtcaacag gtgggggcgc
      301 atcaaggtcg tatcgtccga gatcgggttc acgaggcgga tcgcgtccgc cgggtcgaat
      361 ccgagaaacg cccccagccg ctcccaggta cgacccgcga cgaaggagaa gttcaccgcc
      421 tgtgaaaacc cgcgtcctcg caggaagtcg aaagcccgtt cctggacgtc gacgaaccgg
      481 tcatccccgg aaaattccgg cgccttcgat tcgggatacg tcgtgggaat ggagtcgtac
      541 ccggacagcc gggcaacctc ctccaccagg tcgatctccc gctcgatgtc gaaccggtgc
      601 gtggggaccg tgacgttcca cattcccgtt ccgttatcct cgatcgggaa cccgaggcgc
      661 ccgaagatct ccatgcacgc ttcgggtgcg tataccttac ccatgatccg ggtcgcgcgt
      721 tccggacgga acggcaccgt tcgcggcttt acgttctctc cgccgacgtc gatcactccc
      781 ttcgcgacgg acaggggggc aaaccgggaa aggagcgaca ccgcccggtc cgcggcgtag
      841 acggttcccg ccgggtcgac tccccgctcg aagcggtacg aggcttcgct cgacagtccc
      901 agccgcttcg ccgtccttcg gatcgaggag ggcaagaagt gggcgctctc gaagacgacg
      961 cgcgtcgttc cgtcgacgac ctcgctgttg gccccgccca tcacgccggc gacggccacc
     1021 ggaccttccc cgtccaggat gaggagcatc cctggatcga tctccctctc cgacccgtcc
     1081 agggtcgtaa agctcagcat ttctttcggg gcccgcacat cgatcctcgc cccgcgcagg
     1141 cggtcgaggt cgaaggcgtg catcggctgg cccacttcga gcaacaggta gttggtgacg
     1201 tcgacgatgt tgtttatcgg acggatcccg cacagggtga ggcgccgccg caaccagtcc
     1261 ggggaagggg cgatggtcac gccggagatc gcccgggcgg tgtaccgggt gcacagatcc
     1321 ggatcggaga cgtcgatccg gaccaactcc ccgatcggct ccccctcttc cgggaaggag
     1381 acgtcgggaa gaacgacctt ctctccggtg atggaggcga tctcgcgcgc cacgccgagc
     1441 acgctcaggc agtcgccccg gttcggcgtg atctcgacct cgaggagcca gtcggaaagg
     1501 ccgagcgcgt cggcaagcgg cttaccggga gcggtgtc
//
LOCUS       DASVJL010000214         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_877832, whole genome shotgun sequence.
ACCESSION   DASVJL010000214 DASVJL010000000
VERSION     DASVJL010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877832"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="VF357_04330"
     CDS             complement(<1..1366)
                     /locus_tag="VF357_04330"
                     /inference="COORDINATES: protein motif:HMM:NF039222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX7523405.1"
                     /translation="MLPGELFHPGSVAVVGASRNPEKVGYGVFANLVQAGFQGKIYGV
                     NPGGGELFGHPLYTSIEAIPGPVDLGVFVVPPNAILEGIPRLAAKGMRAGIVISAGFK
                     EIGGAGVALERSLREAATSAGVRVLGPNCLGLIDTHTSLNASFSRGTPPKGFISFFSQ
                     SGALCTAVLDWAIGRNVGFSKFVSLGNKADVSESDILEFLADDPSTRVILGYVESIDD
                     GRRFLRAARNVTPRKPVIIVKAGATAAGARAASSHTGSLAGSDHAYAAAFRQGGVLRA
                     GTVEDLFDLALGFAMQPLPRGDRLLIVTNAGGPGILAADTAERLGIPLAGVSASLRER
                     ILPAVPPTASLANPVDIIGDARADRYRSVLSALRDEPSVDAVLVLLTPQSMTEPEETA
                     RATVSAFAGSDKTVLASFLGEATVASSRRILSDGGVPNYAVPERAVQTLSAMLRYSRI
                     RSADR"
     gene            complement(1386..>1604)
                     /locus_tag="VF357_04335"
     CDS             complement(1386..>1604)
                     /locus_tag="VF357_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX7523406.1"
                     /translation="LEALEFGAPPHGGIAFGLDRLAMLFSGAKSLRDVIAFPKTQKAT
                     CLMTEAPSGVDLKQLRELSIRVTAPEKK"
BASE COUNT          254 a          547 c          576 g          227 t
ORIGIN      
        1 gccggtccgc agagcggatg cgggagtatc gaagcatcgc ggaaagggtc tgcaccgccc
       61 gctcgggaac cgcgtagttg gggacgccgc cgtcgctgag gatccggcgc gacgaggcca
      121 ccgtggcctc cccgaggaag gaggcgagca cggtcttgtc ggagccggcg aaggcggaaa
      181 cggtcgccct cgcggtctcc tccggctccg tcatcgactg cggcgtcagc aggacgagga
      241 cagcgtcgac cgacggctcg tcccggagcg ccgagaggac actcctgtac cggtccgcgc
      301 gggcgtcccc gatgatgtcc accgggttcg ccaggctcgc cgtgggcggc actgcgggaa
      361 ggatccgctc ccgaagggag gcggagaccc cggcgagggg aatgccgagc cgctccgccg
      421 tgtccgccgc gaggatcccc ggaccacccg cgttcgtcac gatcaggagc cggtcccccc
      481 gggggagcgg ctgcatcgcg aatccgaggg ccaggtcgaa gagatcctcg accgtccccg
      541 cccggagcac cccgccctgc cggaacgccg ccgcgtaggc gtggtcggag ccggcgagac
      601 tgccggtgtg ggaagaggcg gcgcgggcgc ccgctgcggt ggctcccgcc ttcacgatga
      661 tgaccggctt gcggggcgtc acattccggg ccgcgcgcag gaaccggcgg ccgtcgtcga
      721 tgctctcgac gtacccgagg atgacccggg tcgacggatc gtcggcgagg aactcgagga
      781 tgtccgactc cgagacgtcg gccttgttcc cgaggctcac gaacttggag aagccgacgt
      841 tccggccgat ggcccagtcg agcaccgcgg tgcagagcgc cccggattgg gagaagaagg
      901 agatgaatcc cttggggggc gtcccgcggg agaacgaggc gttcagcgag gtgtgcgtgt
      961 cgatcaggcc gaggcagttg gggcccagca cccggacccc cgcggatgtc gccgcctcgc
     1021 gcagggatcg ttcgagggcg acccccgccc ctccgatctc cttgaatccg gcggagatga
     1081 cgatacccgc gcgcatcccc ttcgccgcga gccgggggat cccttcgagg atggcgttcg
     1141 gcgggacgac gaagacgccc aggtcgacgg gcccggggat cgcttcgatg gaagtgtaca
     1201 gcgggtggcc gaaaagttct ccgccgccgg gattcactcc gtagatcttc ccctggaacc
     1261 cggcctgcac gaggttggcg aacaccccgt acccgacttt ctccgggttt cgggacgccc
     1321 cgacgacggc gaccgatccg ggatggaaca gttcgccagg gagcatggcc cctctccggt
     1381 tcgggctact tcttttccgg cgccgtcacc cggatcgaaa gctcgcgcag ctgcttcaag
     1441 tcgaccccgg agggagcctc cgtcatgagg catgtcgcct tctgggtctt cgggaaggcg
     1501 atcacgtcgc gaagcgattt cgcgcccgag aagagcatcg ccagccggtc cagcccgaag
     1561 gcgatcccgc cgtgcggcgg cgcgccgaat tccagcgctt ccag
//
LOCUS       DASVJL010000215         4254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1252428, whole genome shotgun sequence.
ACCESSION   DASVJL010000215 DASVJL010000000
VERSION     DASVJL010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252428"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..977)
                     /locus_tag="VF357_04340"
     CDS             complement(24..977)
                     /locus_tag="VF357_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7523407.1"
                     /translation="MSGQPVSNPAVDGARAGLLDREGALAKLMIAPAILYIVAMIGVP
                     FVLAVLYSLSDATTGDPTLKFVGLRNFAAALEDPVFRLSLWNTVFFTLVSQCIVVLLS
                     RLLATALLKTFRGKWLVRFLIMLPWTAPISLGTIGWLWMLDSIFSPIDWVLRYVGLLG
                     APGALLGAGTNLYYLGIPGLAISSIILVDVWRILPLATVIQLAGLSSIPKDLLEAAEV
                     DGASPWRRTLMITIPLTLPITSIAFLFGVVFTFTDLVVVYVLTRGGPVHMTQVLSSWT
                     FFKGIEGGDLAQGAAISLFMFPVLAAVAILILRTARRTEVV"
     gene            complement(974..2011)
                     /locus_tag="VF357_04345"
     CDS             complement(974..2011)
                     /locus_tag="VF357_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523408.1"
                     /translation="MTMGRVEIRGISKLFGTVRAVDHIDLATNEGEFLVLLGPSGCGK
                     TTLLRMIAGIEEPTEGDMIIDGRVVTNLPPRMRNIAMVFQSYALYPHLTVYKNIAFPL
                     RARRVEKEAVRKKVEWAAGMFKIGRLLDRKPRELSGGERQRVALARAMVREPSVFLLD
                     EPLSNLDALLRTSAREELRQFQRRVGVTTIYVTHDQVEAMGLGDRIVVMNQGKIRQIG
                     TPMEIYLWPADTFVATFVGSPPMNLLEQGDTLLGFRPETFLPADAVDGSEGVVTYPFE
                     VTYLEYLGAERLVYGNVGEKSHARHVVSRLPGSLTLPLEAGKTYPFAAALRDLRRFDK
                     KSGLYKEGVPS"
     gene            complement(2087..3508)
                     /locus_tag="VF357_04350"
     CDS             complement(2087..3508)
                     /locus_tag="VF357_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7523409.1"
                     /translation="MAKRSLLTRRDFIKAAGIGAVTMGVAPTIFIPRQAHGASKELKI
                     LVWSHFVPRFDKDWYDGYVQKWGEANGVKVTVDHINLAEIPSRTAAEISTGQGHDLIE
                     WIAPPSQFEPSVLDLGDVVKEAEKRYGKQHPLCRRSSYNPNSKKFYCFIPGWTIDPGN
                     YRKSLWEKAGKESGPDTWEDLVTYGGKIKKDQGIQLGIGLSQELDSNMAARALLWSYD
                     SSIQDTKENVVLNNPKTLEAVIYMARLFKESMVPEVFAWTAVSNNQALIAGRASYILN
                     SISAYRSAQKQVPEIAKDIYFTPALKGPRGTRYSCEHVIYAYVVPKYSKNVDSAKKFL
                     LDLVGNYDQAMYASELYNSPAFFDAPIPSGGRGYAAVKGAKKVRDLHNAWFSEDPFAL
                     PGEDKGKLLFLKDAEKWCTNLGHPGPANPAEGEVFATFVLPNMMANAARGLSAETSIE
                     QATLLTKTIFAKWRKKGLIGGKA"
     gene            4048..>4254
                     /locus_tag="VF357_04355"
     CDS             4048..>4254
                     /locus_tag="VF357_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7523410.1"
                     /translation="MKKVLIGALVFGMIATQAFAADTIKIAITGPFSGGSAPMGASMR
                     DGAKLAIAEINAAGGISVGGKKMKF"
BASE COUNT          756 a         1324 c         1398 g          776 t
ORIGIN      
        1 cgcgagtttc cgcacgttcg ccatcagacc acctccgtcc gtcgcgctgt ccgcagaatc
       61 aggatcgcga ccgccgcgag cacggggaac atgaacaggg agatggccgc cccctgggcg
      121 aggtccccgc cctcgatccc cttgaagaac gtccaggagg agagcacctg ggtcatgtgc
      181 accggcccgc cgcgggtcag tacgtagacg acgaccagat cggtgaaggt gaagacgacg
      241 ccgaagagga aggcgatgct cgtgatggga agggtgagcg ggatcgtgat catcagggtc
      301 cggcgccacg gggaggctcc gtccacctcg gcggcctcca ggaggtcctt ggggatggag
      361 ctcaaccccg ccagttggat gaccgtcgca agggggagga tgcgccacac gtcgacgagg
      421 atgatcgagg agatcgccag cccggggatc cccaggtagt agaggttcgt ccccgctccc
      481 aggagggcgc ccggggctcc gagaagtccg acgtagcgaa ggacccagtc gatggggctg
      541 aagatcgagt cgagcatcca gagccagccg atcgtcccga gggagatcgg cgccgtccac
      601 gggagcatga tgaggaaccg gacgagccac ttcccccgga acgtcttgag gagggcggtg
      661 gcgagcagcc gggacagaag gacgacgatg cactgggaga ccagtgtgaa gaagacggtg
      721 ttccacaggg atagccggaa gacgggatcc tccagcgccg cggcgaagtt ccgcaggccg
      781 acgaacttga gagtggggtc cccggtggtg gcgtcgctca ggctgtacag gaccgccagg
      841 acgaagggga cgccgatcat cgccacgatg tagaggatcg cgggggcgat catcagcttc
      901 gcgagggcgc cttcccggtc aaggagtccc gcccgcgcgc cgtcgaccgc cgggttcgaa
      961 accggctgcc cgctcacgac ggcacacctt ccttgtagag cccgctcttc ttgtcgaagc
     1021 ggcgcagatc cctcaaggcc gccgcgaagg ggtacgtctt cccggcctcc agcggcaggg
     1081 tgagggaccc cgggaggcgg gagacgacgt ggcgggcgtg ggacttctcc ccgacgttgc
     1141 cgtacacgag ccgctcggcc cccagatact cgaggtacgt cacctcgaag gggtaggtga
     1201 cgaccccctc cgacccatcg acggcgtcgg cgggaaggaa cgtctccggg cggaagccaa
     1261 ggagggtgtc accctgctcg aggagattca tcggagggga cccgacgaag gtcgccacga
     1321 aggtatccgc gggccacagg tagatctcca tcggcgtacc gatctgtcgg atcttcccct
     1381 ggttcatcac cacgatccgg tcccccagcc ccatcgcctc gacctggtcg tgggtgacgt
     1441 agatggtggt gacccccacc cgccgctgga actgccggag ctcctcccgc gccgaggtgc
     1501 gaaggagggc gtccaggttg gacagcggct cgtccagcag gaagacgctg ggctcccgga
     1561 ccatggcgcg ggccagcgcc acgcgctgcc gctccccccc ggagagctcc cgcggcttcc
     1621 ggtcgagcag gcgcccgatc ttgaacattc ccgccgccca ctcgaccttt ttccggaccg
     1681 cctccttttc cactctccgg gcgcgcaacg gaaaggcgat gttcttgtac accgtcaggt
     1741 ggggatagag ggcgtagctc tgaaagacca tcgcgatgtt ccgcatccgg ggcggcaggt
     1801 tcgtgaccac ccgtccgtcg atgatcatgt ctccctcggt cggctcctcg atcccggcga
     1861 tcatccgcaa gagggtggtc tttccacacc ccgaggggcc caggaggacc aggaactccc
     1921 cctcgttcgt cgcgaggtcg atgtggtcga cggcccgcac ggttccgaac agtttcgata
     1981 tgccacggat ttccacacgc cccatggtca tcgctgcctt ccgtgttttg ttggaatgcg
     2041 ctcgggagga cggcggcatc ccgccgtcct cccgggccga agcggactag gctttccccc
     2101 cgatcagccc cttcttgcgc cacttggcga agatggtctt ggtcaggagc gtggcctgct
     2161 ctatcgacgt ctcggccgat aggccacggg cggcgtttgc catcatgttc ggcaggacga
     2221 aggtcgcgaa gacctctccc tccgccgggt tcgccgggcc ggggtgcccg aggttggtgc
     2281 accacttctc ggcgtccttg agaaagagca gctttccctt gtcctccccc ggtagggcga
     2341 acggatcctc cgagaaccag gcgttgtgga ggtcgcgaac cttcttcgcc cccttgacgg
     2401 ccgcgtagcc ccggcccccg gaggggatcg gggcgtcgaa gaaggcgggg gagttgtaaa
     2461 gctcgctcgc gtacatcgcc tggtcgtaat tcccgaccag gtcgaggagg aactttttcg
     2521 cgctgtcgac gttcttcgaa tacttcggca cgacataggc gtagatcacg tgctcgcagc
     2581 tgtaccgcgt cccgcgaggc cccttgaggg cgggagtgaa gtagatgtcc ttggcgatct
     2641 cgggaacctg tttctgcgcg gagcggtatg ccgagatgga gttgaggatg taggatgcac
     2701 ggccggcgat gagcgcctgg ttgttggaga cggcggtcca ggcgaacacc tcggggacca
     2761 tagactcctt gaacagccgg gccatgtaga tgaccgcctc cagcgtcttc gggttgttca
     2821 ggaccacgtt ctccttcgtg tcctggatgc tgctgtcgta cgaccacagc agcgcgcggg
     2881 ccgccatgtt cgagtcgagc tcctgggaca ggccgatacc gagctggatc ccctggtcct
     2941 tcttgatctt cccgccgtac gtgaccaggt cctcccacgt atccggcccg ctctctttcc
     3001 cggccttctc ccagaggctc ttccggtagt tgccggggtc gatcgtccag cccgggatga
     3061 agcagtagaa cttcttcgag ttcgggttgt aggagctcct gcggcagaga ggatgctgtt
     3121 tcccgtaccg cttctcggcc tccttgacga cgtcgccaag gtcgaggacg ctcggctcga
     3181 actgggaggg gggtgcgatc cactcgatca ggtcatgtcc ctggccggtg gagatctcgg
     3241 cagccgtgcg ggacgggatc tcggcgaggt tgatgtggtc caccgtgacc ttgacgccgt
     3301 tggcctcgcc ccatttctgc acgtagccgt cgtaccaatc cttgtcgaac ctgggaacga
     3361 agtgcgacca gacgaggatc ttcagctctt tcgaggcgcc atgggcctgc ctcgggatga
     3421 agatcgtcgg agcgaccccc atcgtgaccg cgccgatccc cgccgccttg atgaaatcgc
     3481 gtcgggtcag taaactccgt tttgccattt tccgctcccc ttttccggcg tgcggtgccc
     3541 gcgtcgccgg attgcccagt tcggtccctg aaaacttgct ccgtgcggtg tattccccga
     3601 ttgaacgcct gatatatcgc tttgatgcgt gcggcaatgc atatttaatc accggtacga
     3661 cggatggtca agaatccaaa gcgagtttaa aacaggttta tagcggcggc agatggcccc
     3721 atgtcggcgg gatcaaccga taaccaatgt ggaaaccggt cctcgatcgg tccggagtga
     3781 aagaaggggg aagaccgaag cggctttcca cccatccctt caggacggcc ccttccgggg
     3841 agctggcgtc ctgcatccag ccctgcagga accgcagata gatgttttta acgggcccgg
     3901 ctcccgagag ggaaggactc cttcctctgt gattccaatt gaattcttgc cgatgccgtg
     3961 taaaaatgtg cgacattgtg gtcgcgggac tggcccggtg accgaaggga ttgtggggtt
     4021 attcaaccgc gataggagga gggatacatg aaaaaggttt taatcggagc gctcgtgttc
     4081 ggcatgatcg ccacccaggc tttcgcagca gataccatca agatcgcgat caccggtccg
     4141 ttttccggag gctcggcgcc catgggggcg tccatgcggg acggcgcgaa actggcgatc
     4201 gcggagatca acgcggccgg cgggatcagc gtgggcggca agaagatgaa gttc
//
LOCUS       DASVJL010000216         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_970845, whole genome shotgun sequence.
ACCESSION   DASVJL010000216 DASVJL010000000
VERSION     DASVJL010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970845"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VF357_04360"
     CDS             complement(<1..392)
                     /locus_tag="VF357_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523411.1"
                     /translation="MTKLKRLLMNVPVLGFLALLALAAPVFAAETLGTPALPPGVTPE
                     QAEAVRQAIQSGAPIPPEVQKALEAHPELKNQLPPDVKQKVEEKLTEKKSGAESAAKT
                     PASETSAEAFGLLPAYDWKTSQYVGRLFL"
     gene            complement(475..924)
                     /locus_tag="VF357_04365"
     CDS             complement(475..924)
                     /locus_tag="VF357_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7523412.1"
                     /translation="MTVGTTHGERIVVPDASVILKWIDRFPGEKDRDRADALLDAWLS
                     GRMEIVVPPLWAFEVANVLGREDPLGAGPIMEELLGYRFEEADPTPELCRAAFGLMKD
                     FRVTFYDAVYHAVALLRKGVFVTADEAYLRKVGRRGGVALLRDFNPV"
     gene            complement(921..1166)
                     /locus_tag="VF357_04370"
     CDS             complement(921..1166)
                     /locus_tag="VF357_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523413.1"
                     /translation="MPTSARKLNFMIRNEIARELEALVPAGERSRTVNDALAKELLAI
                     RRRMITLRLRAARGKGPALGTEKIVAALRRDRGRDTK"
     gene            complement(1252..>1512)
                     /locus_tag="VF357_04375"
     CDS             complement(1252..>1512)
                     /locus_tag="VF357_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7523414.1"
                     /translation="LTAERRRRLTRAQTFQCLEMLRSLPVVVDESTSSRAMGDILSLA
                     RDQNLSVYDAAYLELSIREALPLATRDRTLAGAAKRCGVPLK"
BASE COUNT          260 a          505 c          487 g          260 t
ORIGIN      
        1 aggaagaggc ggccgacgta ttgcgaggtc ttccagtcgt acgccgggag cagcccgaac
       61 gcctcagccg acgtctcgga cgcgggggtc ttcgcggcgg attcggcccc ggacttcttt
      121 tcggtgagct tctcctcgac cttctgcttc acgtcggggg ggagctggtt cttgagctcc
      181 gggtgcgcct cgagcgcctt ctgcacctcg ggggggatcg gcgcgccgga ctggatcgcc
      241 tgccgcaccg cctcggcctg ctccggggtg acgccggggg gaagggccgg ggtgcccaag
      301 gtttcggccg cgaagaccgg ggccgcgagc gccaggagcg caaggaaccc cagaaccggg
      361 acgttcatga gaagtcgctt gagtttcgtc atcgtttgcc ttccgaaggg ggtatcggtc
      421 gtctgtacgg gggaaacgat cctgcccaca tcctatccat gcgatgagaa ccggtcaaac
      481 cgggttgaaa tcccggagca gggcgacccc gccccgacgg ccgaccttgc ggaggtacgc
      541 ttcatcggcg gtcacgaaaa cccctttccg gagaagcgcc acggcatgat aaacggcgtc
      601 gtaaaacgtg acacggaaat ccttcatcag cccgaaggcc gcacggcaca actccggggt
      661 cggatccgcc tcctcgaacc ggtacccgag cagctcttcc atgatcggac cggcgcccag
      721 gggatcctcg cgcccaagga cattggcgac ctcgaacgcc cacagagggg gcacgacgat
      781 ttccatacgt ccgctcagcc aggcatccag aagggcgtcg gcgcggtccc ggtccttctc
      841 cccgggaaag cggtcgatcc atttcaggat caccgaggcg tccggaacca cgatccgttc
      901 gccgtgcgtc gtccccaccg tcacttcgtg tccctgcccc tgtctctccg gagggcggcg
      961 acgatcttct ccgtaccgag ggcgggcccc ttcccgcggg ccgcccggag gcgcaaggtg
     1021 atcatcctcc gacggatggc aaggagctcc ttcgcaagag cgtcgttgac ggtccggctg
     1081 cgctcgccgg cgggaaccag cgcttcgagt tcccgggcga tctcgttccg gatcatgaaa
     1141 ttcagtttcc gcgcggaagt cggcatcggg tcatctcccg ggatggggta acatcattac
     1201 ccctccatcc tacctattcg gcggcaacct gtcaacgcgc accctccatc ttcatttcag
     1261 gggaactccg cagcgtttcg cggcacccgc gagggtacgg tctcgggtcg caagggggag
     1321 cgcctcgcgg atcgacagtt ccaggtacgc agcgtcatat acggagaggt tctgatccct
     1381 ggccagcgac aggatatcgc ccatcgcccg ggaagacgtg ctttcgtcca cgacgacggg
     1441 aagggaacgc agcatttcca ggcactggaa cgtctgggca cgcgtgagcc gccgacggcg
     1501 ctccgccgtc ag
//
LOCUS       DASVJL010000217         5473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1816471, whole genome shotgun sequence.
ACCESSION   DASVJL010000217 DASVJL010000000
VERSION     DASVJL010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816471"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VF357_04380"
     CDS             complement(<1..453)
                     /locus_tag="VF357_04380"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523415.1"
                     /translation="MTDPSKRPAPADTGELVPEGGLLAKIPIGYSRRHLLLPCRTSSG
                     EIVLLSGGKPEAREAIDEMRFLAGSTRVVCAPENEVLARIDAAYERAHSPENEIVEGL
                     PGEWDLDPTAAIEETRDLLESPDEAPVIRFVHAVLFRAIRERSSDIHFE"
     gene            complement(450..2855)
                     /gene="gspD"
                     /locus_tag="VF357_04385"
     CDS             complement(450..2855)
                     /gene="gspD"
                     /locus_tag="VF357_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX7523416.1"
                     /translation="MRIPVLLIAGLIALGPAAHAAETPSATEAPPAAVTSPPPAPSPA
                     GQDNAAPGATTDVVGAPPVPAPAAAPQASPPPAPPPVVQENVAPGTIGGVVPPPVPAP
                     AGAPLAPPGPAPSAAPAAAPAPSMVPVAAPAPSMVPVVAPAPSMVPVTQSPTYLSIDF
                     TDVELPVLIKFMSEQTKKNFIFDERVQGKITIISPRRVTLDEAYDVFLYVLQAKGFTT
                     VSQGNTIKIIAAREARQDSIHTGVSKDTAPAEFITRLIPLQFLEVTEVVPLVTPLVSK
                     DGMVSAFGSSNTLMLIDSRANIDRIVRILEEVDNVGMTGILRIYPLSFAIAADAVKTL
                     TSIYIEGGPAGAPAAAGRARGVSPSRRGAGAKFLADVRTNSVIVFAGREMQEDVADLL
                     KQIDIPASGGSGKINVYYLENADAEEVSKVLASLSEKRAGASTTPPPPGARTAAPAVT
                     GAAVVSAELEGGVKVTPDKATNSLIIVASTNDYETLLGVIKKLDIRRKQVYVEAVIME
                     ITINKGRDLGVEFRGAVETGSGDKKGAIIGGTNFDFAGNVNQLFGSLATGNPLVFAGS
                     GLIAGGIAGSVTLPDGTKIPALTAVLRAAQSNNNINILSSPHLLTLDNKEAEIVVGSN
                     VPFITSQARDSTNLANTINTVERKDVGITLRMTPHIHESDFVSLDLFQESSAIAPDSL
                     QNANTVGPTTTKRSAKTSVLVKSGDTVVLGGMMQETFTNNTSQIPLLGDIPLLGALFR
                     FKSISRQKTNLLIMLTPRIIRDPGQMLDRSAERQRRMTEEFDRAKRDVERTFPDPKPG
                     ENR"
     gene            complement(2870..3784)
                     /gene="gspC"
                     /locus_tag="VF357_04390"
     CDS             complement(2870..3784)
                     /gene="gspC"
                     /locus_tag="VF357_04390"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HEX7523417.1"
                     /translation="MRKPVQLAVLGISIVVAAVSLGMTFAKYLSLGAYAPKAATGPAA
                     APQAPVVPTMPPEQWTNLFAPAQGMKLPSRLPGTNAKSASQAPRTAFVLVGTIDSSSS
                     SARRAILWANGMKLPKAFREKEEVEPGAVLSSVERDKVWLTRGGEREMLEILPVGSKA
                     RASLSPPVGELKSGVPGTPQGAGTSSTPAGDIRVTRLADNRFSIDEAGVTQLTTNINQ
                     YMTQVRLIPFFEGNKSAGYRIAAIRPGTTFEQLGFQGGDVLQQVNGLDLSSPEKLYTI
                     FQNLKDEKKVSVNILRQGQKNTLMYEIR"
     gene            complement(3861..4571)
                     /locus_tag="VF357_04395"
     CDS             complement(3861..4571)
                     /locus_tag="VF357_04395"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX7523418.1"
                     /translation="MRKPVYLAILGISIAVAAASLGVTVTRYLGLRAYSPKEMAQASV
                     PPAGPNVPATPPEQWNNLFAPGDGMKMASRLPTMSGKTASQAPRTRFVLVGTIVSSAP
                     SYSRAILWANGMKEPKAFREKEEVEPGAFLASVERDKAWITRGNEREKLEILPVGSPE
                     RATATAAPTVAPPVAAEASLPAVPAGTSALPGVAPATRPTPVTTQAVGASNPSMDPED
                     QESARPQERGRRSRGIRR"
     gene            4716..5426
                     /locus_tag="VF357_04400"
     CDS             4716..5426
                     /locus_tag="VF357_04400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7523419.1"
                     /translation="MRQLAAAVLCVALTLSSSVYAADAQTLKSDRDKVGYSIGMDIGS
                     NLKNQQIEIDPEALSIGLKDSYRGGKTALTEEEAQRTIKTFQQQMAVKQQEMRKKLAE
                     KNRLEGEKFLAENARKEGVKTLPSGLQYKVLKPGTGKSPKAIDSVKVNVKGNLLDGTV
                     IESIYEGEEWMPGDPVSFEVSTAIAGWKEALPLMKEGAKWQLVVPPNLAYGERGDGRN
                     IGPEATLIYELELLSIEK"
BASE COUNT          893 a         1684 c         1943 g          953 t
ORIGIN      
        1 ctcgaagtgg atgtcgctgg accgctcccg gatcgcacgg aagaggacgg cgtggacgaa
       61 gcggatgacc ggcgcctcgt cgggcgactc gagaaggtcc cgcgtctcct cgatggcggc
      121 cgtcgggtcg aggtcccatt ctccgggaag cccctccacg atctcgttct ccggggagtg
      181 cgcccgctcg tacgcggcat cgatccgggc gagcacctcg ttctccggcg cgcagacgac
      241 ccgggtggat ccggcgagga accgcatctc gtcgatcgcc tcccgggcct ccggctttcc
      301 ccccgacagg aggacgatct ccccggacga ggtccggcag gggaggagca ggtgcctgcg
      361 ggagtacccg atcgggatct tcgcgagcag gcccccctcc gggacgagct cgcccgtgtc
      421 ggccggcgcc ggccgtttcg acggatcggt catcgattct ccccgggctt cgggtcgggg
      481 aacgtcctct cgacgtccct tttcgcccgg tcgaactcct cggtcatcct cctttgccgc
      541 tccgccgacc tgtccagcat ctgccccggg tcgcggatga tccggggagt gagcatgatg
      601 aggaggttcg tcttctggcg cgatatggac ttgaacctga agagagcccc gaggagcggg
      661 atgtccccga ggagggggat ctggctggtg ttgttcgtga acgtctcctg catcatcccg
      721 cccagcacca ccgtgtcccc gctctttacc agcacgctcg tctttgcgga ccgcttcgtc
      781 gtggtggggc ccaccgtgtt cgcattctgc agggaatccg gcgcgatggc ggacgactcc
      841 tggaagagat cgaggctcac gaagtcgctc tcgtggatat ggggcgtcat gcgcagcgtg
      901 atgccgacgt ccttccgttc gaccgtgttg atcgtgttgg cgaggttcgt cgagtcccgg
      961 gcctggctcg tgatgaaggg gacgttcgag ccgacgacga tctcggcctc cttgttgtcg
     1021 agggtcagca ggtgcgggga ggagaggatg ttgatgttgt tgttcgattg ggccgcccgc
     1081 agcaccgcgg tgagggcggg gatcttcgtc ccgtcgggaa gggtcacgct tcccgcgatc
     1141 cccccggcga tcaggccgga ccccgcgaag acgagcgggt tgccggtggc cagcgacccg
     1201 aacagctggt tcacgtttcc cgcgaagtcg aagttggtcc caccgatgat cgcgcccttt
     1261 ttgtcgccgc tcccggtttc gacggcgccg cggaactcca cgccgagatc cctccccttg
     1321 ttgatcgtga tctccatgat caccgcctcg acgtagacct gcttgcggcg gatgtcgagc
     1381 ttcttgatga ctcccagcag cgtctcgtag tcgttcgtcg atgcgacgat gatcagggaa
     1441 ttggtggcct tgtcgggggt gaccttcacc ccgccctcga gctcggcgga gacgacggcc
     1501 gccccggtca ccgccggggc ggcggtccgc gcgcccggag gggggggcgt cgtcgaggcg
     1561 ccggcacgct tctccgagag ggaggcgagg accttcgaca cctcttcggc gtccgcgttt
     1621 tcgaggtagt agacgttgat ctttccgctg ccgccggagg cggggatgtc gatctgtttc
     1681 agcaggtccg ccacgtcttc ctgcatctcg cgccccgcga acacgatcac gctgttggtg
     1741 cggacatccg ccaggaactt cgccccggcg ccgcggcggg aagggctgac cccgcgggcg
     1801 cgtcctgccg cggccggcgc gccggcgggg cctccctcga tgtagatcga ggtcagggtc
     1861 ttcacggcat cggccgcgat ggcgaaggac agggggtaga tccgaaggat gccggtcatc
     1921 cccacgttgt cgacctcttc gaggattctg acgatccggt cgatattggc gcgcgagtcg
     1981 atcagcatca gcgtgttgga ggaaccgaac gcggagacca tcccgtcctt ggaaaccagc
     2041 ggcgtaacca gcgggacgac ctcggtgacc tcgaggaact gcagcgggat gaggcgggtg
     2101 atgaactccg cgggggcggt atctttcgaa acgccggtgt ggatggagtc ctggcgcgct
     2161 tcccgcgcgg cgatgatctt gatcgtgttc ccctgcgaaa cggtggtgaa ccccttcgcc
     2221 tgcagcacgt agaggaagac gtcgtacgcc tcgtccagcg ttacccggcg gggggagatg
     2281 atcgtgatct tcccctgcac ccgctcgtcg aagatgaagt tcttcttcgt ctgctcgctc
     2341 atgaacttga tgaggaccgg cagctcgacg tcggtgaagt cgatcgacag gtacgtgggg
     2401 gattgggtca cgggaaccat ggatggggcg ggagccacca cgggaaccat ggatggggcg
     2461 ggagccgcca cgggaaccat ggatggggcg ggagccgccg cgggcgccgc ggagggtgcg
     2521 ggccccgggg gcgccagggg agcgccggcc ggcgccggaa ccggcggcgg cacgacgccg
     2581 ccgatcgtgc cgggagcgac gttctcctgc acgacggggg gaggtgcggg cggcggagac
     2641 gcctgggggg cggcggccgg cgccggaacc gggggcgccc cgacgacgtc cgtcgtggcc
     2701 ccgggagcgg cgttgtcctg cccggcgggg gagggtgcgg gcggcggaga cgtcacggcg
     2761 gccgggggcg cctcggtcgc cgaaggtgtc tcggccgcgt gcgcggcggg accgagggcg
     2821 atcaggccgg cgatcagcag gacaggaatt cgcatgggat tctcccggat catcggattt
     2881 cgtacatcag tgtgttcttt tgcccttgac gaaggatgtt caccgacacc tttttttcgt
     2941 ctttcaggtt ctggaagatg gtgtagagct tctcggggga ggaaaggtcc agcccgttca
     3001 cctgctggag gacgtcccct ccctggaacc cgagctgttc gaaggtggtg ccggggcgga
     3061 tggcggcgat ccggtagccg gccgatttgt tcccttcgaa aaacgggatc aggcggacct
     3121 gggtcatgta ttggttgatg ttggtggtca attgcgtcac acccgcctcg tcgatggaga
     3181 accggttgtc cgcgaggcgg gtcacgcgga tgtctccggc cggcgtggag gaggtcccgg
     3241 ccccctgcgg tgtgccggga accccgctct tcagctcccc gacgggcggg gaaagagagg
     3301 cgcgcgcctt gctgcccacg gggaggattt cgagcatctc gcgctcgccg ccccgggtga
     3361 gccacacctt gtcccgctcg acggaggaaa gaaccgcccc gggctcgacc tcctcctttt
     3421 cacggaacgc tttcggcagc ttcatcccgt tcgcccacag gatcgcacgg cgggcggaag
     3481 aactggagga gtcgatggtc cccacgagga cgaacgcggt gcgcggcgcc tgggaagcgg
     3541 atttcgcgtt cgtcccgggg agccgcgacg gcaatttcat cccctgcgcg ggggcgaaga
     3601 gattggtcca ctgctccggc ggcatcgttg ggacgaccgg cgcctggggg gcggcggcgg
     3661 gccccgtcgc cgccttcggg gcatatgcgc ccaaggaaag gtacttcgca aacgtcatgc
     3721 cgagggaaac cgccgccacg acgatggaaa tccccaatac cgccaattgt accggttttc
     3781 gcatccactc tccggaagat cggatataac tctatatatt ggacgcatcc cggggagttc
     3841 gtcaacagcg ccgcgtgccg tcaccgacgg atcccacggg atcgcctgcc ccgttcctgc
     3901 gggcgggcgg actcctgatc ctccgggtcc atggaaggat tggaggcgcc caccgcctgc
     3961 gtcgtgacgg gtgtcggtcg cgttgcgggc gcgaccccgg gaagagcgga cgtgcccgcg
     4021 gggacggccg gaagggacgc ctcggccgcc acggggggag cgaccgtcgg ggccgccgtc
     4081 gccgtagcgc gttcggggct tcccacgggc aggatctcca gcttttcccg ctcgttgccg
     4141 cgcgtgatcc acgccttgtc gcgctcgacg gaggcgagga acgccccggg ctcgacctcc
     4201 tccttttccc ggaacgcctt cggctccttc atgccgttcg cccagaggat cgcccggctg
     4261 taggaagggg cggaggatac gatcgtcccc acgagaacga aacgggtacg cggcgcctgg
     4321 gaagccgtct tcccgctcat cgtggggagc cgggacgcca tcttcattcc atccccgggg
     4381 gcgaagaggt tgttccactg ctccggcggt gtggcgggga cgttgggtcc ggcggggggc
     4441 acggacgcct gcgccatttc cttcggggag tacgcgcgca ggccgaggta gcgcgtcacg
     4501 gtgacgccga gcgaagcggc cgccacggcg atggaaatgc ccaatatggc caggtagacc
     4561 ggcttccgca ttccttcccc gggaaataag atttgtgtcc ctatattgac cgcatttccc
     4621 ggatcccgtc aaccgtgctc cgaggatcaa tttctcctcg gggaacgtgt aagatgatcg
     4681 ggcgaccact ttttcttctg aaaaggagag ggcagatgag gcaacttgcg gctgcggtgc
     4741 tgtgcgtggc gctgacgctc tccagctcgg tgtacgccgc ggatgcgcag actctcaaga
     4801 gtgacaggga caaggtcggc tacagcatcg ggatggacat cgggtccaac ctgaagaacc
     4861 agcagatcga aatcgacccg gaggcgctgt cgatagggtt gaaggacagt taccgcggcg
     4921 ggaagacggc tcttaccgag gaagaggcgc agcgcacgat caagaccttt cagcagcaga
     4981 tggcggtaaa acagcaggag atgcggaaaa aactcgcgga gaagaaccgg ctggaagggg
     5041 agaagttcct ggcggagaac gccaggaagg aaggggtaaa gacgttgccg agcgggttgc
     5101 agtacaaggt gctcaagccc ggcaccggga aaagccccaa ggcgatcgat agcgtcaagg
     5161 tgaacgtaaa aggaaatctc ctcgacggga cggtgatcga gtccatttac gaaggcgaag
     5221 aatggatgcc gggcgacccg gtttccttcg aggtctccac ggcgatcgcg ggctggaagg
     5281 aggcgctgcc gttgatgaag gaaggcgcca agtggcagct cgtcgtcccg ccgaaccttg
     5341 cgtacgggga gcggggggac ggccgcaata tcgggccgga ggccacgctg atctacgagt
     5401 tggagctgct ctcgatcgag aaatgacccg gacgccgaag ggccaggcac ggatcctggc
     5461 ccttcgggat ttc
//
LOCUS       DASVJL010000218         4871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_783675, whole genome shotgun sequence.
ACCESSION   DASVJL010000218 DASVJL010000000
VERSION     DASVJL010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783675"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..792
                     /locus_tag="VF357_04405"
     CDS             142..792
                     /locus_tag="VF357_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEX7523420.1"
                     /translation="MPERFAVVGTGGHAKVVVDALLASGHEVLGFYDDNPVLLGAEPV
                     PGVKVLGDTLELPGGLEKGKGMVILAVGENRIRCRLSRRLSVPYGVACAPSAVLGSGV
                     RIGKGSMILPSATVNIDTVIGAHVILNTSCSVDHDCAVGDFVHIGPGAHLGGGVVVGE
                     GTFLGLGSSVIPGIRIGRWSVIGAGAVVTKDIPDNCTAVGAPAKVIKTREEGWHLG"
     gene            789..1925
                     /locus_tag="VF357_04410"
     CDS             789..1925
                     /locus_tag="VF357_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7523421.1"
                     /translation="MTGPPLPKRIYLSPPHMGPAERELLLDAFDSNWIAPLGPHVDAF
                     EREFCETVGVSYAAALSSGTAVLHLALLMLGVGPGDEVPVSTFTFAASANVVKYLGAT
                     PVFLDSDRETWNMDPGLLEEELSACAQRGKLPKAVIAVDLYGQSADYDRLQAACGRYG
                     VPMVEDAAEALGATYKGKPAGSFGVMAAFSFNGNKIITTSGGGMLVSSDRSLVDRARH
                     LATQARDPAPHYQHSMIGYNYRMSNLLAAVGRGQLRVLKDRISRKREIFRYYRKALGD
                     LPGISFMPTAGYGEPNYWLTCILIDPARFGATRENIRLALEKENIESRPLWKPMHLQP
                     AFKDCRVRDGSVSEGIFRDGLCLPSGTALTDVQLERVAGIIRAV"
     gene            2041..3984
                     /locus_tag="VF357_04415"
     CDS             2041..3984
                     /locus_tag="VF357_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX7523422.1"
                     /translation="MKFFLTLGIFILANGLAYLIRFDFVVPANMVVVMRRTIPILVIA
                     KALGFLASGQFRGWWRYVSIRDVFPIAAGSALGTVLFAVGVWWFWGPFYVPRSIYLLD
                     WGITMGLVLGARYLIRMGREGLGRRGQVKERRVLIVGAGAAGQMIAREIVGNVSLGME
                     QVGFIDDDREKIGTRIHGVKVMGGHNQIAAICRKYDVNEVIIALPSASASIVRHIVEH
                     CREVSATFRILPGIGDLIDGKVSVHALRNLDLEDLLGRDPVALDAELLERHITGRTVM
                     VTGAAGSIGSELCRQIARLSPGRLILFEIAESPLFFLEFEMRTQFPTVALVPVIGDVR
                     DRGRVEEIVRAHRPAIIFHAAAYKHVPMMEIHPVEAVKTNVLGTRIVAEAAAKFGVER
                     FVLVSTDKVVRPTNVMGATMRLAELVIHNIANRQGPTVFVAVRFGNVLGSVGSVIPIF
                     RRQLSTTGKLTVTHKDASRYFMLIPEAAGLILQAGAMGEGGEVFVLDMGEPVKIVNLA
                     KNLIRLSGKEEGVDAEIVFTGLRPGEKLHEELIVEGEDVSGTVHPKVMKLIGSEKMPP
                     SWSKQLEELIVCAITGERCSVVEKLDALVKGYRPDYGFHGVDVPVSKTASRSRAPVGP
                     RPPLQVRPLDPPLSCPSPVLARF"
     gene            4085..4357
                     /locus_tag="VF357_04420"
     CDS             4085..4357
                     /locus_tag="VF357_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HEX7523423.1"
                     /translation="MDPKWFLVKTKALNEPRVHKRLTGAGYEVLFPKISRKSKRHRGL
                     FEMRPLFPTYLDAIFVEEMPDRERVVLLLDAVSSYKLTIEKDSLER"
     gene            4614..>4871
                     /locus_tag="VF357_04425"
     CDS             4614..>4871
                     /locus_tag="VF357_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523424.1"
                     /translation="MSLLWGSFRNVPVLGFVLAALALFAAPAHAADLFLTGEREIYLA
                     VDKLNAMGQLPGFLANTRPYSVAAVRAALDKNASSSPGIGFD"
BASE COUNT          869 a         1430 c         1649 g          923 t
ORIGIN      
        1 acaaccggtc gttcgcgctg gatgtgaaga tacttgcgat gaccgcctgg aaagtccttg
       61 ggcagaaggg gatcagcgct gagggagatg ccacgatgcc ggagttcatg gggactgcct
      121 ctaccagagg gaaatccgaa aatgcctgag cgtttcgcgg tcgtcggtac cggcggccac
      181 gccaaggtgg tggtggatgc gttgctcgct tccgggcatg aagtgctggg gttctatgac
      241 gataatccgg tcttgctggg ggcggaaccg gtaccgggcg tgaaggtgtt gggtgacacg
      301 ttggagttgc cgggggggct ggaaaagggg aaaggcatgg tcatcctggc ggtcggcgag
      361 aaccgtatac gttgccggtt gtcgcggcgg ctttccgttc cgtacggtgt tgcgtgcgct
      421 ccctccgcgg tgctcggtag cggcgtccgg atcggaaaag gaagcatgat ccttccgtct
      481 gcgacggtga acatcgacac cgttatcgga gcacatgtga tcctgaacac gtcctgttcc
      541 gttgatcacg actgcgcggt cggggatttc gttcacatcg ggccgggggc tcatctggga
      601 gggggagtgg ttgtggggga gggaactttc ctcggccttg gatcgagcgt gatcccggga
      661 attcggatcg gccgctggtc cgtgatcgga gccggtgccg tcgtcacgaa ggatattccc
      721 gacaattgca ccgccgtcgg ggcccccgca aaggtcatca agacccgcga agaaggatgg
      781 cacctggggt gacgggcccg ccgctgccga agcggatcta cctctccccc ccgcacatgg
      841 gaccggccga gcgggagctg ctgctcgatg ccttcgattc gaactggatc gcaccgttgg
      901 ggccccacgt ggacgccttc gagcgggaat tctgcgagac cgtcggggta tcctacgccg
      961 cggccctctc cagcgggacg gcggttctgc accttgcact gctgatgttg ggcgtcggcc
     1021 ccggggacga ggtcccggtg tctacgttta cgttcgcggc ctccgcgaac gtcgtgaagt
     1081 acctcggggc aaccccggtc ttcctcgact cggaccggga gacctggaac atggacccgg
     1141 ggctcctgga ggaggaactt tcggcatgcg cgcaacgggg gaaactcccg aaagcggtca
     1201 tcgccgtgga tctgtacgga cagtccgcgg actacgaccg gctccaggct gcctgtgggc
     1261 gatacggggt accgatggtg gaagacgcgg ccgaggcact cggggccacc tataagggga
     1321 aacctgcggg gtctttcggc gtgatggcgg cgttctcctt caatgggaac aagatcataa
     1381 ccacatcggg tggggggatg ctcgtctcgt cggatcggtc gctggtcgac cgggcccggc
     1441 acctggcgac gcaagccagg gaccccgcgc cgcattatca gcacagtatg atagggtata
     1501 actaccggat gagcaacctg ctggccgccg tgggccgggg acagttgcgg gtgttgaagg
     1561 acaggatttc ccgaaagcgg gagatcttcc gttattatcg gaaggccctc ggcgatcttc
     1621 ccggcatctc cttcatgccg accgcagggt atggtgagcc gaactattgg ctgacctgta
     1681 tcctgatcga tccggcccgt ttcggagcga cgagggaaaa catccgcctc gcgctggaaa
     1741 aggagaacat cgaatcccgg ccgctgtgga agccgatgca cctgcagccc gccttcaagg
     1801 attgcagagt gagagacgga tcggtatccg aagggatctt ccgggacggc ctgtgtcttc
     1861 ccagcggaac ggcgttgaca gatgtgcaac ttgagcgggt tgcagggatc attcgtgccg
     1921 tttaaagtag atagatcgat ggaatatcgg atctcgtaaa ggagccgtat gaatcttcgc
     1981 gaaagaatcg cccggtggtt ttccccgttt ctggatgtcg cgatccggag ccggacgacg
     2041 gtgaaattct tccttaccct cgggatcttc atcttggcga acggcctcgc ctatctgatc
     2101 cggttcgatt tcgtcgtacc ggcgaacatg gtcgtcgtga tgcggcggac gatcccgatc
     2161 ctggtgatcg cgaaggcgct cgggttcctc gcttccgggc agttccgcgg ctggtggcga
     2221 tatgtgtcca tcagggacgt gttcccgatt gcggccggtt ccgcactggg cacggtcctc
     2281 ttcgcggtgg gcgtctggtg gttctggggg ccgttctatg tgccgaggtc gatctacctg
     2341 ctggattggg ggatcacgat ggggctggtc ctcggcgccc ggtacctcat ccggatggga
     2401 agggaagggc tgggaaggag agggcaggtg aaggagcgac gcgtcctgat cgtcggagcg
     2461 ggcgcggccg ggcagatgat cgcaagggag atcgtcggga acgtctctct ggggatggag
     2521 caggtcgggt tcatcgacga cgaccgggag aagatcggta cgcggatcca cggcgtgaaa
     2581 gtgatggggg ggcacaacca gatcgccgcc atctgcagga aatacgatgt caacgaggtc
     2641 atcatcgcgc tcccgtccgc ttcggcgtcg attgtgcgtc acatcgtgga gcattgccgc
     2701 gaggtgtccg ccacgttccg gatcctgccc ggcatcggcg acctgatcga cggcaaggtg
     2761 agcgtccacg ccctgcggaa cctggacctc gaggacttgc ttgggcggga cccggtggcg
     2821 ctggatgccg aattgcttga gcggcacatc acggggcgca cggtgatggt gaccggggcg
     2881 gcggggtcga tcggttcgga gctgtgccgc cagatcgcgc ggctgtcgcc ggggcggctg
     2941 atcctgttcg agatcgccga gagcccgctg ttctttctcg agtttgagat gcggacgcaa
     3001 ttcccgaccg tggccctggt tccggtgatc ggcgacgtcc gcgaccgggg gagggtcgag
     3061 gagatcgtcc gcgcgcaccg tcccgcgatc atcttccacg cggcggcgta caagcacgtg
     3121 ccgatgatgg agatccaccc cgtggaggcg gtgaagacga acgtgctggg gacccggatc
     3181 gtggcggagg cggccgcgaa gttcggcgtg gagcgattcg ttctcgtctc cacggacaag
     3241 gtggtgcgcc cgacgaacgt gatgggggcg acgatgcggc tggcggagct cgtgatccac
     3301 aacatcgcca accggcaggg gccgacggtg ttcgtcgccg tccggttcgg caacgtcctt
     3361 ggcagcgtgg ggagcgtgat cccgatcttc cgcaggcaac tgtcgacgac ggggaagctg
     3421 accgtcaccc acaaggatgc gtcgcgctac ttcatgctga tccccgaggc ggctgggctg
     3481 atcctccagg cgggcgcgat gggggagggc ggcgaggtgt tcgtcctcga catgggcgaa
     3541 ccggtgaaga tcgtcaacct ggcgaagaac ctgatccgcc tgagcgggaa agaggagggc
     3601 gtggacgccg agatcgtgtt cacggggctg cgcccgggcg agaagctgca cgaggagctg
     3661 atcgtcgagg gcgaagacgt gagcggcacc gtccacccga aggtgatgaa gctgatcggg
     3721 agcgagaaga tgcccccctc atggtcgaag cagctcgagg agttgatcgt atgcgctatc
     3781 accggggagc ggtgcagcgt cgtcgagaag ctggacgccc tggtgaaagg gtatcgcccg
     3841 gactacggat tccacggcgt cgacgttccc gtctccaaga cagcttcgcg aagccgagct
     3901 cccgtcggac cccgaccccc gcttcaagtc cgtccactag atcccccact ctcctgcccc
     3961 tcccccgtac ttgcacggtt ttgatatgat tcacgtataa tccggcgtac gccccatttc
     4021 aacgagacgt gcatcctgtg cgcgtgcgga tgtcccacac ggcgatgtcc gcgggagggt
     4081 gtgcttggac ccgaagtggt tcctcgtcaa gacgaaggcg ctcaatgaac cacgcgtcca
     4141 caagcgcctg accggcgccg gctacgaagt tctcttcccg aaaatcagca ggaaatcgaa
     4201 gcgccaccgg ggcctcttcg agatgcgccc cctcttcccg acctacctcg acgcgatctt
     4261 cgtggaggag atgcccgacc gcgagcgcgt ggtcctgctc ctcgacgccg tgtcaagcta
     4321 caaacttact atcgagaaag attctttgga acggtaaatt atcaatgttt cccgtgcttc
     4381 agggattcgg gaggatatat aatatgaagg atattcaccg tttcatcccg ggaatggaaa
     4441 cagagactcg cgccccgcag aaattggccg tatccccagc ggttttcggt gtttcccgag
     4501 gccgcatggg cggtagcctg cccccacccc agaaccggga cgttcctaaa actcccacag
     4561 aacggggaca cacattgccc gatgggaaat gccgcaggag aacgaaacca ggagtgtccc
     4621 tcctttgggg gagttttagg aacgtcccgg ttctggggtt cgtccttgcg gccctcgccc
     4681 tgttcgccgc gcccgcccac gctgccgacc tcttcctcac cggcgagcgg gagatctacc
     4741 ttgccgtcga caagttgaac gcgatgggcc agctccccgg cttcctcgcc aacacgcgcc
     4801 cgtactccgt cgccgccgtc cgcgccgccc tcgacaagaa cgcctcctcc tccccaggca
     4861 tcggcttcga c
//
LOCUS       DASVJL010000219         4179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_314777, whole genome shotgun sequence.
ACCESSION   DASVJL010000219 DASVJL010000000
VERSION     DASVJL010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314777"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VF357_04430"
     CDS             <1..405
                     /locus_tag="VF357_04430"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEX7523425.1"
                     /translation="RYDGGHKRAASLIGTLGPFVEWVRVCPEVDCGLPVPREAMRLVW
                     DPKHPHLVTNRTGIDHTERMERWAKARLEELSPLDLCGYICKKDSPSCGMEPVGAFTR
                     MFSERFPHLPVEEEGRLADPLRCKRFLQRVKP"
     gene            complement(395..>4179)
                     /locus_tag="VF357_04435"
     CDS             complement(395..>4179)
                     /locus_tag="VF357_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7523426.1"
                     /translation="GNDGLHWVFEKGFVLRLLSGMAEGTLDEGIPALLACRLLRDASF
                     PDEKLSIERQFSAAVKGTLEAYRFYQSLTDKTRKQMAEMYERHPPGDPLFRLFASLET
                     EGNPARASHMIRNMTARTHSYRHVRYPDTALAGKVVVITGGGTGMGRSLALEAARRGG
                     NVVITGRRHAPLLETKADMDDLIRHLGLTNQTLLVQGDVSDPKYVGEMFEQIEKEFGR
                     IDVLYNNAGVSGPVVFGSAYEEGHFDEYREAVNVHLTGSWMASLEAARIMEGQPGGGT
                     IVMVGTFYSESIHHHVLHAYPGRLPYTSAQSAKLALGDYLAWALAGKKITVLSLNPSA
                     VSTERIQRGSGVFDKGSKARARIGRNVSPEALERDTLERTVGHAFVDPRDFAALALEV
                     VEGPFRRTIGGIRLPMGGVTYEQPPGVFPSPAVLSRYPDLVGKVALLVVGEALSGDVP
                     LIESSAAALARAGAAVILSGGRPEILEPIALKINARGGEGTATVSPVNLSHPAQVQEL
                     FDGLPRVDLLLYFTGSVDWKRPLTNLPHDEWTARVDRFGLIPRFLCWQAERRMDRDGT
                     DGTIVLVGPDLSGAPTIRERNLVQVFQAMLRPAVATEAMERALMRKAQAEGTSPGPVA
                     NVNIGLVLPGRTDGRNRQARPEATAASVLWFLDDGKRVSGAVLLPDEQNAIAALPPDP
                     VEIPGSASGKVAVVTGGIRNLGKVISLRLAAERATVVIGSRYPPTGSRDAGEAEKARG
                     ELASADDVLTRMRRSGGRALWVHTDVAQPGSVRALLSEARNRFGRIDLLVNNAGAGGN
                     FSRVGDVMREHRENFSAVLAANFLGPWEAIVAAREILRTQPGGGSIVNISTHYADHPY
                     LFRTIYTVSKILLKALTKASRAELAADGISVTDVAPTLIAGPRMEWVMRNYATKFSAG
                     FDDFPGLSPAARKVLTEAFLRSFDGAVSAVAREGAALSFLAALRSQKMPKGGRERIES
                     WYGRIGEWFRSTVPAAPPGNEEVAEAVLFAAKDGRFLENPFLAITTLPPFSSFPPSPV
                     PGRVISGGETGTIVSTGDAGTLHRRLHEALSGKGVRVTTLTDAGLPDGQARISRPDAY
                     PPAKGARGRAQETQQRTLDLSDPRVVEPWLDNALAGAPPPTFVVLIVGVMAGGKGVLD
                     GSGEDRLRFLEDVQKTVTLFAESARAVRDGGHLVVVGPPETIGEGPLMLSALRQTVRT
                     FLAEQHFLPAVKTVRLSLLAGPVPGAERDTEHEVIAILEGAQPPRVEPIPVGYVRA"
BASE COUNT          672 a         1407 c         1419 g          681 t
ORIGIN      
        1 cgctacgacg gcgggcacaa gcgcgctgcc tccctgatcg gtacactggg gcccttcgtc
       61 gagtgggttc gggtctgccc ggaggtcgac tgcgggcttc ccgtcccgcg cgaggcgatg
      121 cgactcgtct gggatccgaa gcacccgcat ctggtcacga acaggaccgg gatcgatcac
      181 acggaacgga tggagcggtg ggcgaaggcc cggctcgaag aactgtctcc cctcgatctg
      241 tgcgggtaca tctgcaagaa ggactcaccc agctgcggga tggaaccggt gggggccttc
      301 acgcgcatgt tcagcgagcg gttcccccat cttccggtgg aggaggaggg acgccttgcc
      361 gacccgctgc ggtgcaagag gttcctccag cgagtcaagc cctgacgtat ccgaccggga
      421 tcggttcgac ccgggggggc tgcgctcctt cgagaattgc gatcacctcg tgctccgtat
      481 cccgctccgc ccccgggacg ggtccggcga ggagggagag gcgaaccgtt ttcaccgccg
      541 ggaggaaatg ctgctcggcg aggaacgtcc ggacggtctg gcgcagggcg gacagcatca
      601 acggtccctc cccgatcgtt tccggaggtc cgacgacgac gaggtgaccg ccgtcccgca
      661 cggcgcgcgc ggactcggcg aacagggtga ccgtcttttg gacgtcttcg aggaaccgca
      721 ggcggtcttc cccggaccca tcgaggaccc cttttcctcc cgccatgacg ccgacgatca
      781 ggacgacgaa cgtcggcgga ggcgcgccgg cgagcgcatt gtcgagccac ggctcgacga
      841 cccggggatc ggacaggtcc agggtgcgtt gttgcgtttc ctgagcgcgg ccccgggccc
      901 cctttgccgg aggatacgcg tcgggccggc tgatccgcgc ctgaccgtca ggcagcccgg
      961 catccgtgag cgtcgtcacg cggaccccct ttccggagag cgcctcgtgc aggcggcggt
     1021 gcagcgttcc ggcatctccg gtagacacga tcgtacccgt ttcgcctcct gagatgaccc
     1081 tgccgggcac cggcgacggc gggaacgagg agaagggggg cagggtggtg atcgcgagga
     1141 acggattttc gaggaaccgc ccgtccttgg cggcgaacaa caccgcctcc gccacctcct
     1201 cgttccccgg cggggccgcg ggaaccgtgg agcggaacca ctccccgatc cggccgtacc
     1261 acgactcgat ccgctcgcgc ccgcctttcg gcatcttctg cgaccggagg gctgcgagga
     1321 acgacaacgc ggcaccttcc cgcgccacag cggaaacggc gccgtcgaac gatcgaagga
     1381 aggcctccgt caggaccttg cgggctgcgg gggaaagacc ggggaaatca tcgaaaccgg
     1441 cggagaactt cgtcgcgtag ttccgcatca cccactccat cctcggaccg gcgatgagcg
     1501 tcggcgcgac gtcggtgacg gagattccgt cggccgccag ttccgcgcgc gaggcctttg
     1561 tcagcgcctt caataaaatt ttcgataccg tgtagatcgt ccggaagagg tacgggtgat
     1621 cggcgtagtg ggtcgagatg ttgacgatcg agcccccgcc aggctgggta cgcagtattt
     1681 cccgcgcggc gacgatcgcc tcccacggcc ccagaaagtt cgcggcgagg accgcggaaa
     1741 aattctcgcg gtgttcccgc atcacatccc cgacccgaga gaagttaccc cccgcgccgg
     1801 cgttgttcac cagcaggtcg atgcggccga accggtttct cgcctccgag agcaacgcgc
     1861 gcacgcttcc tggctgcgcg acgtccgtgt gcacccacag tgcgcgaccg ccggagcgcc
     1921 gcatccgcgt caggacgtcg tcggccgagg caagctctcc tcgagccttt tcggcttccc
     1981 cggcatcccg cgaacccgtt ggtggatacc ggcttccgat gacgaccgtc gccctctccg
     2041 ccgccagtcg aagggagatc accttgccaa gattccggat cccaccggtg acgaccgcca
     2101 ccttccccga tgccgagccg ggaatttcca cggggtccgg gggcagtgcc gcgatggcat
     2161 tctgctcgtc gggcaggaga acggctcccg agacgcgctt gccgtcgtcg aggaaccaga
     2221 ggacggaggc tgcggtcgct tcgggccggg cctgccggtt cctgccatcg gtgcgccccg
     2281 gaaggacgag cccgatgttc acgttcgcca cggggccggg ggaggttcct tcggcttgcg
     2341 cttttcgcat cagcgcgcgt tccatcgcct cggtcgccac cgcggggcga agcatcgcct
     2401 ggaagacctg cacgaggttc cgctcccgga tcgtcggggc acccgagaga tccggcccca
     2461 ccaggacgat cgtgccgtcc gtcccgtccc ggtccatccg gcgctcggcc tgccagcaaa
     2521 ggaaccgggg gatcaacccg aaacggtcga cgcgcgcggt ccattcgtca tgcggcagat
     2581 tcgtcagcgg ccgtttccag tccacgctcc cggtaaagta caagaggagg tcgacccgcg
     2641 gcaggccgtc gaacagttcc tgcacctgcg cggggtggga gaggttgaca ggggagaccg
     2701 tggcggtccc ttctcctccg cgcgcgttga tcttcagcgc gatcggctcg aggatttcag
     2761 gccggccgcc ggagaggatc acggcggcgc cggcccgcgc aagtgcggcc gcggatgact
     2821 cgatcagggg gacgtctccg gaaagggctt ctccgaccac cagcagcgcg acctttccga
     2881 ccaggtcggg gtaccgcgag agcacggcgg gggagggaaa gacgccgggt ggctgctcgt
     2941 aggtgacgcc gcccatcggg aggcggatcc cgccgatcgt cctccggaaa gggccttcga
     3001 cgacctcgag cgcgagcgcg gcgaagtccc gggggtcgac gaaggcgtgc cccacggtcc
     3061 gttccagggt gtcgcgctcc agcgcttccg gggaaacgtt gcgcccgatt ctggcgcgcg
     3121 ctttcgatcc cttgtcgaag acgccgctgc cccgctggat ccgctccgtg gagaccgccg
     3181 acgggttgag ggagagtacc gtgatctttt ttcccgcgag ggcccacgca aggtaatcgc
     3241 cgagggcgag tttggcggat tgcgcggagg tgtacgggag acgcccgggg taggcgtgca
     3301 ggacgtggtg gtggatgctc tcgctgtaaa aggttcccac catgacgatc gtcccgccgc
     3361 ccggttgccc ctccatgatc ctcgccgcct cgagcgacgc catccaggaa ccggtgaggt
     3421 ggacgttgac cgcctcccgg tactcgtcga aatggccctc ctcgtacgcc gagccgaaca
     3481 ccaccgggcc cgagacgccg gcgttgttgt acaggacgtc gatgcggccg aactccttct
     3541 cgatctgctc gaacatctcc cctacgtatt tcggatcgga cacgtccccc tgtacgagaa
     3601 gggtctggtt cgtgagaccg aggtggcgga tgaggtcgtc catgtcggcc ttcgtttcga
     3661 ggagcggggc gtgccgccga ccggtgatca ccacgttccc gccgcgtcgc gccgcttcga
     3721 gggcgaggct gcgccccatc cccgtgccgc cgccggtgat cacgaccacc tttcccgcga
     3781 gggcggtgtc cgggtaccgg acgtggcggt acgagtgggt tcgcgccgtc atgttccgga
     3841 tcatgtggct ggcgcgggcg ggattcccct ccgtctccag cgacgcgaaa aggcggaaca
     3901 acggatcgcc cggaggatgc cgctcgtaca tttccgccat ctgcttccgc gtcttatcgg
     3961 tgagcgactg gtagaaacgg tacgcctcga gcgtcccctt gacggcggcc gagaattgcc
     4021 gctcgatcga gagcttctcg tccgggaagg aggcgtcccg caggagacgg catgcgagga
     4081 gcgcgggaat cccttcgtcg agagtgccct cggccatccc ggagagaagc cggagaacga
     4141 atcccttctc gaacacccag tgaaggccgt cgttcccat
//
LOCUS       DASVJL010000220         3446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1065750, whole genome shotgun sequence.
ACCESSION   DASVJL010000220 DASVJL010000000
VERSION     DASVJL010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065750"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1330
                     /locus_tag="VF357_04440"
     CDS             131..1330
                     /locus_tag="VF357_04440"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7523427.1"
                     /translation="MSGPLSGVRVLDLSRVLAGPFCTMILGDLGAEVIKVERPEGGDD
                     TREWGPPFAAGESAYYLCVNRNKKSVALDLKSPKGIAAVRALSARSDVVLENFRVGVA
                     DQLGLGYEALAKGNPGLVYCSITGYGQTGPYRDLPGYDFILQAMSGLMSITGEKSGDP
                     MKLGVATVDLTTGLYAAVAILAALRRRDSTGVGQRIDLALMDSALSWLANVGENYLVS
                     AEVPGRFGNGHATIVPYQVFRSKDGYLAVGIGNDRQYRKFCEIAGAPGLSSDERFSTN
                     PARVRNRETLVPLLERVFATRPTAEWIERLWNEGIPAGPINTVDRALEDPGTLARGMV
                     VEMPHPKAGSVRLIGSPMKFSATPVEYRQPPPLLGEHTEEVLRDVLGMPAEEIRQLVG
                     TPEPGRS"
     gene            complement(1360..2244)
                     /locus_tag="VF357_04445"
     CDS             complement(1360..2244)
                     /locus_tag="VF357_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7523428.1"
                     /translation="MITRLTEKIWIVEGGKGGRYPYAHSLYVADGGGILVDAGSDPAE
                     IERLRKENGIAAVVMTHYHEDHITFLSRLPDTPMWSSAADAPALESLETLLAWYGVLG
                     NEWDAFYRKLFDEKFPFAPRKVARRIADGEELRFGDARAVAVVAPGHTPGHLCLHFPD
                     DGILFLGDYDLTAFGPWYGDAPCGIDAFRKSARRLAAIAAERFVVSHEGPVHRGPIAE
                     KMDAYLAVIDRREEALRDYVREPRTRAEIIARRLIYGPGRDGPWFDYGEWALLSKHLD
                     GMLARGEAVLRDGAYVLP"
     gene            2362..2781
                     /locus_tag="VF357_04450"
     CDS             2362..2781
                     /locus_tag="VF357_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523429.1"
                     /translation="MKKILLLPFCLSREAQEMAESLAAAEGYVVVVARSTARALAEVR
                     RHAGPPGSGAPVRIVGVVCDGRAKKVWAGLVLLKARQWGKRVLRRRVRRIELARVAIT
                     GGTKSLFGRRQCHVGRNELDTFGLRRALRGGDTFMTV"
     gene            complement(2768..>3446)
                     /locus_tag="VF357_04455"
     CDS             complement(2768..>3446)
                     /locus_tag="VF357_04455"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX7523430.1"
                     /translation="TLRWVFLVSAGVAAIAVVLSREAVLLVYQRGAFTIDDTMRTASA
                     LSAFCLGIPFWCAQAIVSRGFFAMRDTWTPTLVGTGAWIVALPVYYLLQQSYGVFGLA
                     LASTAGIFLYAGALYGILMRRTVGRRGVPEVIEYGKLALAAALAGGTGYLLLGASSRF
                     LAWETVFGSMIRLAGGASAIGGIYFLLAFLFHSGTARTIHRREDLLHPPGSIPDGGSD
                     GNGVTPS"
BASE COUNT          557 a         1141 c         1234 g          514 t
ORIGIN      
        1 atgtgcaacc tcgagtcggt cgacacctac gaggggacgt acgagatcca caccctcgtg
       61 atggggaaag acgtgaccgg catcgatgcc gtgtcgtgag aaggaagatc gtgcgcggcg
      121 gaggattgcg atgagcggcc cattgtcggg ggtccgcgtc ctcgacctgt cgcgggtcct
      181 ggcgggtccc ttctgcacga tgatcctcgg ggacctcggg gccgaggtga tcaaggtcga
      241 acggcccgag gggggggacg acacgcgcga gtggggcccc cccttcgccg ccggggaatc
      301 cgcgtactac ctgtgcgtca accggaacaa gaagagcgtc gccctggacc tcaagtcccc
      361 gaaggggatc gcggccgtgc gggcgctttc cgcccgcagc gacgtcgtgc tcgagaactt
      421 ccgggtgggg gtggcggacc agctggggct cggctacgag gcgctcgcga aagggaatcc
      481 cggtctcgtc tactgcagca tcacggggta cggacagacc ggtccgtacc gcgatcttcc
      541 ggggtacgac ttcatcctcc aggcgatgag cgggctgatg agcatcacgg gggaaaaaag
      601 cggagatccg atgaagctgg gcgtggcgac ggtcgacctg accacggggt tgtacgccgc
      661 ggtggcgatc ctggcggcct tgcgccgtcg cgattccacc ggggtggggc agcgaatcga
      721 cctcgccttg atggactcgg ccctctcgtg gctcgcgaac gtgggggaga attacctcgt
      781 ttccgccgag gttccgggac ggttcggcaa cgggcacgcc accattgttc cgtaccaggt
      841 gttccgctcc aaggacggtt acctcgccgt cgggatcggc aacgacaggc agtaccggaa
      901 gttctgcgag atcgccggcg cgccggggct ttcctccgac gaacggtttt cgacgaatcc
      961 ggcgcgggtc cgcaacaggg aaacgctcgt ccccctgctg gagcgcgtgt tcgcgacgcg
     1021 gccgaccgcc gagtggatcg aacgtctctg gaacgagggg atcccggcgg ggccgatcaa
     1081 cacggtggac cgggcgctgg aggacccggg cacccttgcg cgggggatgg tggtcgagat
     1141 gccgcacccc aaggccggtt cggtgcggct gatcggctcc ccgatgaaat tttccgcgac
     1201 ccccgtggag tatcgccagc cgccgccgct gctcggagag cacacggaag aggtgcttcg
     1261 cgacgtcctc gggatgcccg cggaggagat ccggcaactc gtcggaaccc cggaaccggg
     1321 acgttcctag aaccccagga acatcccggt tccggggttc tacggcagga cgtaggcgcc
     1381 gtcgcggaga accgcctccc cgcgggcgag catcccgtcg aggtgcttgg agaggagcgc
     1441 ccactcgccg tagtcgaacc agggaccgtc ccggccgggg ccgtagatca ggcgccgcgc
     1501 gatgatctcc gcgcgggtgc gcggctcccg tacatagtcc cggagcgcct cctcccgccg
     1561 gtcgatcacg gcgaggtacg cgtccatctt ctccgcaatc gggccgcggt ggaccggccc
     1621 ctcgtgggag accacgaacc gttccgcggc gatcgcggcg aggcggcggg cggacttccg
     1681 gaaggcgtcg atgccgcacg gggcgtcgcc gtaccacggg ccgaacgccg tcaggtcgta
     1741 gtcgccgagg aagaggatcc cgtcgtcggg gaaatgcagg cacagatgcc cgggcgtgtg
     1801 gccgggggcg acgacggcga ccgcgcgcgc atcgccgaag cgcagttcct ccccgtccgc
     1861 gatccttcgc gcgactttcc tcggggcgaa ggggaatttc tcgtcgaaga gtttccggta
     1921 gaaggcgtcc cactcgttcc cgaggacgcc gtaccacgcc agcagcgtct cgagcgattc
     1981 gagggccggg gcgtccgcgg ccgaggacca catcggcgtg tctggcaaac gggagaggaa
     2041 tgtgatgtgg tcctcatggt agtgggtcat cacgaccgcc gcgatcccgt tctcctttcg
     2101 cagccgctcg atctccgccg gatcggagcc ggcgtccacg aggatgccgc ccccgtccgc
     2161 cacgtacagg gagtgcgcgt acgggtaccg cccgcccttc ccgccctcca cgatccatat
     2221 cttctccgtc agccgcgtga tcatcctcgc cttccgtcac cgtccaccgt gggaacgttt
     2281 tatcgcaagc ggggcctgcc cccgcgagga accctgcgcc gaggagtccc ccgcgccgcg
     2341 catccgcgat atgatgtccg cgtgaagaag atcctgctgc tcccgttctg cctttctcgc
     2401 gaggcgcagg aaatggcgga atccctcgcc gcggcggaag ggtacgtcgt cgtggtggcg
     2461 cgctcgacgg cccgggcgct ggcggaggtg cgcaggcacg ccgggcctcc cgggagcggc
     2521 gccccggtgc ggattgtcgg cgtcgtgtgc gacgggagag cgaagaaagt gtgggcgggc
     2581 cttgtgctgc tgaaagcgcg gcagtggggg aaacgggtct tgcggcggcg cgtccgccgg
     2641 atcgaactgg cccgggtggc gatcacgggc ggcacgaagt cgctgttcgg tcggcgtcaa
     2701 tgccatgtgg gccgcaatga gctcgatacc ttcgggctgc gccgcgcact gcggggcggc
     2761 gacaccttca tgacggtgtg accccgtttc cgtcgctccc cccgtcggga atcgaccctg
     2821 gcgggtggag caggtcctcg cgccgatgga tggtccgggc cgttccgctg tggaacagga
     2881 aggcgaggag gaagtagatc ccgccaatcg ccgacgctcc gccggccagc cggatcatcg
     2941 acccgaacac ggtttcccac gcgaggaacc gcgaagacgc gcccagcaag aggtatccgg
     3001 tccctccggc gagcgccgcg gccagcgcca gcttcccgta ctcgatcacc tcgggcactc
     3061 cccgccgccc caccgtccgc cgcatcagga tcccgtagag cgctcccgcg tagaggaaga
     3121 tccccgccgt gctggcgagc gcgaggccga agacgccgta cgactgctgc aggaggtagt
     3181 acacggggag ggcgacgatc cacgcaccgg ttcccacgag cgtcggggtc caggtgtccc
     3241 gcatcgcgaa gaatccgcgg gagacgatcg cctgggcgca ccagaagggg atcccgaggc
     3301 agaaggcgga cagggcggag gcggtgcgca tcgtgtcgtc gatcgtgaac gccccgcgct
     3361 ggtagacgag gaggaccgcc tcccgcgaca ggacgacggc gatggccgcc accccggcgg
     3421 agacgaggaa gacccaccgc agggtg
//
LOCUS       DASVJL010000221         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_878491, whole genome shotgun sequence.
ACCESSION   DASVJL010000221 DASVJL010000000
VERSION     DASVJL010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878491"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..668)
                     /locus_tag="VF357_04460"
     CDS             complement(132..668)
                     /locus_tag="VF357_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523431.1"
                     /translation="MLWLAVGVLATIAVPGGAVFGGRADPVLLADMDGGGSDMNIDLA
                     VRQVTVTPVRAHVGDVVHVDVVIENKAEGSRTTSAELLVNGKVVGSQLFTWGKSSGER
                     LYRLSFDWDTRRVTPGEYRIGAQAFVWEDSSPFDNRLDVTQPVVIAPVDAGFPGGEAA
                     GGSATETDPRFGKSRVGG"
     gene            857..1702
                     /locus_tag="VF357_04465"
     CDS             857..1702
                     /locus_tag="VF357_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX7523432.1"
                     /translation="MLAFAPSAGAADPLRVLTSFLPMEIFTRNVVGDTPGVTVESMLP
                     ASMGCPHDYALTPGDMKKISSADLFVANGLGMEEFLGEPVRRANPRIRIVETARTVLP
                     IHGEHGLGDINPHTWVSPRNAILQVREIEKALSAARPVSARAFRWNADAYVSRLSALA
                     AEFEAAAKTFRRRNIVTFHNVFDYLARDFGLTVVGEIETAPGQEPSAGEIRNLSRTIR
                     ERKVPVVFSEPQYSPKLAEALAREAGVPVRVLDPVATGSPVLTAYEDAMLRNLSTLKE
                     ALSAR"
     gene            1699..>2246
                     /locus_tag="VF357_04470"
     CDS             1699..>2246
                     /locus_tag="VF357_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX7523433.1"
                     /translation="MTASEHIHTLEIRNLTVRKGGVEILSGIDANLRCGEVTALVGPN
                     GAGKTTLLLAILGQVPYTGEIRFCCAAEHGQGAPRIGYVPQRFPLDPDAPITVLDFFA
                     LSAQRRPVFLGASRRTREAARESLERAGVAHLLQSPMGRLSGGELQRVLFALALRDAP
                     DILLLDEPVSGVDVAGEELFCD"
BASE COUNT          397 a          794 c          674 g          381 t
ORIGIN      
        1 tccgtataga acgccatcct acctctctta tcgactataa caggaataat cttgagtgct
       61 taaaaaaaat caccccgcgt ccgaacgaaa tccggatcga gcgcgggggg ttgcaggtaa
      121 aaaaaacgac gttatccgcc gacgcgcgac ttcccgaacc gcggatccgt ctctgtcgca
      181 ctgccgcccg cggcttcccc tccggggaat ccggcgtcga caggcgcgat caccaccggt
      241 tgcgtcacgt ccaaccggtt atcgaagggc gacgaatcct cccagacgaa ggcctgggca
      301 ccgatccggt actcgccggg ggtgacccgc cgggtgtccc agtcgaacga caaccggtac
      361 agccgttctc cggaggactt cccccaagtg aagagctgcg accccaccac cttcccattg
      421 accagcagct cggcgctcgt ggtccgactc ccctcggcct tgttctcgat caccacatcg
      481 acgtggacaa catcccccac gtgggcgcgc accggggtga ccgtcacctg gcggaccgcc
      541 agatcgatat tcatgtcact gcctccccca tccatatccg ccagcagaac cggatccgcc
      601 cttcccccga aaaccgctcc cccgggaacg gcgatcgtcg ccagaacccc gaccgcaagc
      661 caaagcatcc gcctcgatcc cagcatcgcc ctttcctcct gacgggtttt tccgtatcct
      721 gcttcgttgg atgcacggat cggggagaaa gttgtgcccc cgaaattgcg aataccatgg
      781 gaatgcaata tattccatac ggaagcatcg agaaaggaat ccgatcctgg ttgccgctgg
      841 tcttttcatc gccttcatgc tcgccttcgc cccatccgcc ggcgccgccg acccattgcg
      901 cgtcctgacc tccttcctgc cgatggagat cttcacccgg aacgtcgtcg gagacacgcc
      961 gggcgtcacc gtcgaatcga tgctcccggc atcgatggga tgcccgcacg actacgccct
     1021 cacccccggc gacatgaaga agatctcctc cgcagatctc ttcgtcgcga acgggctcgg
     1081 gatggaggag ttcctcggag agccggtccg gcgggcgaac ccgaggatcc ggatcgtgga
     1141 aaccgcccgc accgtcctcc cgatccacgg cgagcacggt ctcggggaca tcaacccgca
     1201 cacatgggtc tccccccgga atgcgatcct ccaggtgcgg gagatcgaga aggcgctctc
     1261 cgcggcgagg ccagtcagcg cgcgcgcctt ccggtggaac gccgatgcgt atgtttcccg
     1321 gctgtcggcg ctggcggcgg agttcgaggc ggcggcgaag acgttccgac ggaggaacat
     1381 cgtcaccttc cataacgttt tcgactatct cgcaagggac ttcggcctga ccgtggtcgg
     1441 ggagatcgag acggcgccgg ggcaggagcc gtctgcggga gagatccgga acctgtcgcg
     1501 tacgatccgg gaaaggaagg tcccggtggt cttctccgaa cctcaatact cccccaagct
     1561 cgccgaggcg ctggcgaggg aggcgggggt ccccgtgcgg gtgctggacc ccgtcgcaac
     1621 cggttcccct gtcctcacgg cctacgaaga cgcgatgctg cggaatcttt cgacgctcaa
     1681 ggaagcgctg tcggcccgat gaccgcgagc gaacatatcc acacgctcga gatccgcaac
     1741 ctgaccgtcc ggaagggagg ggtggagatc ctttcgggga tcgacgcaaa cctccggtgc
     1801 ggggaggtca ccgcccttgt cggccccaac ggcgccggaa agaccaccct gctcctcgcg
     1861 atcctgggcc aagtgccgta cacgggagag atccgtttct gctgcgcggc ggagcacggc
     1921 cagggggccc ctcgcatcgg gtacgtgccg cagcgctttc ccctggaccc ggacgcgccg
     1981 atcacggtac tcgacttctt cgccctctcc gcccagcggc gccccgtctt cctcggcgcc
     2041 tcccgccgca ctcgggaggc cgcccgggag agcctcgagc gggccggcgt cgcccacctg
     2101 ctccaaagcc ccatgggtcg gctttccggc ggggagcttc agcgggtgct gttcgccctc
     2161 gcgctgaggg acgcccccga catcctgctg ctcgacgagc cggtctccgg cgtcgacgtc
     2221 gcgggggagg agctgttctg cgactt
//
LOCUS       DASVJL010000222         4119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1253254, whole genome shotgun sequence.
ACCESSION   DASVJL010000222 DASVJL010000000
VERSION     DASVJL010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253254"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VF357_04475"
     CDS             <1..551
                     /locus_tag="VF357_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX7523434.1"
                     /translation="TDDIIMAGFGGQGILMIGNLLALTAMEEGKHVTYFPAYGVEMRG
                     GTANCTVVVSDEEIGSPVVGRPKGLLIMNGPSMEKFLPMLAPGGDLIINSSLVEQRQV
                     TRNDIRLLPVPADDIARNKIGSRQMASMVALGAYVARSGIVKLSTVFDCLPKVISKKY
                     EKFIPLNVNALKEGAALAGFHA"
     gene            532..1188
                     /locus_tag="VF357_04480"
     CDS             532..1188
                     /locus_tag="VF357_04480"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7523435.1"
                     /translation="MPDFTRDETMDDVLSEMAKESGEPERPAGDPEVPVGEHVRTFRE
                     TLGMTVQQFAERTGFSAALLTQIENRMVSPSLGTLVKIANTFGTTVSSFIGGKEEREF
                     SIVRKEDRTTVSRVGLKEGGKSTYTYESLGAGKAGRKMEPFLVRLQPLSESATARSAH
                     DGEEFLYVLSGRVTVCLENLSDVLEEGDSVYYNSTIPHHVHSADEREALILAVIHAGT
                     "
     gene            1190..1837
                     /locus_tag="VF357_04485"
     CDS             1190..1837
                     /locus_tag="VF357_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523436.1"
                     /translation="MPVRGRVVLEGIRFAAGVASDPSGVVTLWKTATGGKAVGCLPAT
                     PVPELLHAAVLLPVALESPEDLSVFYGQIDAWLVGEDPSPFPAPSVAIPRFAFPRVPP
                     ASVEEALDRIEALAEWVGDLSGSPASEGGLWKSIRAYATRRSLLDTLEERFARETAFL
                     TPEECRDIVRAGIFLPAEAHSRLLSSILGIGHEPAAIAAEEEKGDPLIVLAKRLI"
     gene            1913..2350
                     /locus_tag="VF357_04490"
     CDS             1913..2350
                     /locus_tag="VF357_04490"
                     /inference="COORDINATES: protein motif:HMM:NF020839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7523437.1"
                     /translation="MARDSEGTYAIGEISRLVELSQRTIRYYEEIGLLHSVRRIENGK
                     RVYTDNDVRRLKFINRLKVLGLSLAEMVELEKIYRKQRNNREILPKLLQILDERAVQI
                     DERVAQLVSLKKEIREYQQRLRNKVLQDATDEEPGTTGKEMRS"
     gene            2347..3525
                     /locus_tag="VF357_04495"
     CDS             2347..3525
                     /locus_tag="VF357_04495"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX7523438.1"
                     /translation="MKDVVITGAARTAIGSLMGGLSDLPAPRLGAVTIAEAVSRSGIR
                     KEDVEQVIMGNVLSAGMGQAPARQAGIYAGIPVSAGALTINKMCGSGLKAVMLAAQSV
                     LTEEFDVLVAGGMESMSQAPYLLKKARSGYRLGNDTLYDHMIVDGLWDVYNDLHMGNC
                     AETLAKEHKITREEQDAFAASSYTRALSAIKNGKFTDEIVPVPVPQRKGDPVPFLVDE
                     EPGRGNVPKLPTLRSAFEKNGTVTAGNASTINDGAAALVVMSSDAAKRLGAKPLARIV
                     GYATASLEPVWFTIAPVDAIRTLLQKTGVAKEKVGLFEINEAFAGVAIAAIRGLSLDP
                     ERVNVNGGAVALGHPVGCSGARILTTLLYAMADRGERYGIASLCIGGGEAVAVMVEKL
                     "
     gene            3588..>4119
                     /locus_tag="VF357_04500"
     CDS             3588..>4119
                     /locus_tag="VF357_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX7523439.1"
                     /translation="MSVSKIGILGAGQMGSGIAQVSLMSGLQVVLNDVTEAVLARSRA
                     GIGKGLDILVRKEKISTQEKERALNGLQTTTDPCDFASCDLVVEAATEREELKLSLFR
                     KLDEVVPAGRILATSTSSISITKIAAATKRPAQVIGMHFMNPVPLMKLVEVIPGLQTS
                     PETFEATMSFARALGKE"
BASE COUNT          797 a         1271 c         1346 g          705 t
ORIGIN      
        1 tgacggacga catcatcatg gccgggttcg gcggccaggg gatcctgatg atcgggaacc
       61 tgctggccct cacggcgatg gaagagggga aacacgtcac ctacttccca gcgtacgggg
      121 tggagatgcg gggcggcacg gcgaactgta cggtggtcgt ctccgacgag gaaatcggct
      181 ctcccgtggt tggacgcccg aagggccttc tcatcatgaa cggtccgtcg atggagaagt
      241 tcctccccat gctcgcgccg ggcggggacc ttatcatcaa ctcctccctg gtggagcaac
      301 ggcaggtgac ccggaacgac atacgacttc tccctgtccc cgcggacgac atcgcccgca
      361 acaagatcgg aagccggcag atggcatcga tggtcgccct cggggcatac gtcgcccggt
      421 ccggcatcgt gaagctatcg acggtgttcg attgcctccc gaaggtcatc tcgaagaagt
      481 acgaaaaatt cattcccttg aatgtcaacg cactgaagga aggagccgcc cttgccggat
      541 ttcacgcgtg acgagacgat ggacgacgtc ctgtccgaga tggccaagga gtcgggggaa
      601 ccggaaaggc cggcgggaga tcccgaggtc ccggtggggg agcacgtgcg gaccttccgg
      661 gagacgctgg ggatgaccgt gcagcagttc gccgagagga ccggattctc cgccgcgctt
      721 ctcacgcaga tcgagaaccg gatggtctct ccatccctcg ggacgctcgt gaagatcgcc
      781 aacaccttcg gaaccaccgt ttcctcgttc atcggcggaa aggaagagag ggagttctcg
      841 atcgtccgga aggaagaccg caccaccgtc tcgcgcgtcg gcctcaagga aggggggaag
      901 tccacataca cctatgaatc cctcggagcc ggaaaggcgg ggcggaaaat ggaaccgttc
      961 ctcgttcgcc ttcagccgct ctccgagtcc gccacggcgc gtagcgccca cgacggggag
     1021 gagtttctct atgtcctttc cgggagggtg acggtgtgcc tcgagaacct ctccgacgtt
     1081 ctcgaggaag gggacagcgt ctattacaat tccacgatcc cgcaccatgt ccattccgcg
     1141 gacgaacggg aagcgctcat cctcgcggtg atccacgcag ggacgtaacg tgccggtccg
     1201 cgggcgggtc gtcctggaag ggatccggtt cgctgcgggc gtcgcctccg acccctccgg
     1261 cgttgtgacc ctctggaaaa ccgccaccgg cggaaaagcc gtcggctgcc tcccggcgac
     1321 tcccgtcccc gagctcctgc acgccgccgt cctgttaccg gtcgccctgg aatccccgga
     1381 ggatctttcc gtgttctacg gacagatcga cgcgtggctg gtcggagagg atccctctcc
     1441 gtttcccgct ccctcggtag cgatcccacg cttcgcattc cctcgcgtgc cgccggcgag
     1501 tgtcgaagag gcgttggacc ggatcgaggc gttggcggag tgggtgggcg acttgtcggg
     1561 ctccccggca tccgaggggg ggctctggaa atcgatccgc gcatatgcga cccggcggtc
     1621 gctcctggat acgctcgaag aacgattcgc ccgggagacc gcgttcctga ctccggaaga
     1681 gtgccgcgac atcgtccgcg ccgggatctt ccttccagcc gaggcgcact ctcgcctcct
     1741 ctcctcgatc ctcggcatcg gccatgaacc cgccgctatc gcggcggaag aggagaaggg
     1801 cgatccgctg atcgtactcg cgaaacgcct gatctgatcc cggggaaaat tgcggttgac
     1861 acgacggggc ggcccgggta ccatcttgcc agttaacgta aggtggtgaa gcatggcaag
     1921 agatagcgaa ggaacgtacg cgatcgggga gatcagccgc ctggtcgaac tgtcgcagcg
     1981 cacgatccgt tactacgagg agatcgggct tcttcacagc gtccgccgga tcgagaacgg
     2041 caagcgcgtc tacacggaca acgacgtccg ccgcctgaaa ttcatcaacc gcctgaaggt
     2101 cctcggtctc tccctcgcgg agatggtcga actcgaaaaa atctaccgga agcagcgcaa
     2161 caaccgggag atcctgccga agcttctcca gatcctcgac gagcgcgccg ttcagatcga
     2221 tgaacgtgtc gcccagctcg tctcgctgaa gaaagagatc cgggaatacc agcagcgcct
     2281 gcgcaacaag gtcctgcagg acgcaaccga tgaagaaccg ggaacgaccg gaaaggagat
     2341 gcgctcatga aggatgtcgt catcaccggg gccgcaagga ccgcaatcgg gtcgctgatg
     2401 gggggactgt ccgatcttcc ggccccccgg ctcggcgccg tcacgattgc ggaggccgtt
     2461 tcacgaagcg ggattcgcaa ggaggatgtg gagcaggtga tcatgggaaa cgtcctctcc
     2521 gcggggatgg gacaggcgcc cgcccgccag gccgggatct acgccggcat cccggtgtcg
     2581 gccggggcgc tcaccatcaa caagatgtgc gggtccggcc tcaaggccgt catgctcgcg
     2641 gcccagtccg ttctcacgga agagttcgat gttctggtcg ccggcgggat ggagtcgatg
     2701 agccaggcgc cgtacctgct gaaaaaggca cgttccggat accggctggg aaacgacacc
     2761 ctctacgacc acatgatcgt cgacggcctt tgggacgtct acaacgacct ccacatgggg
     2821 aattgcgctg agaccctggc gaaggaacac aagatcacgc gcgaggaaca ggacgcgttc
     2881 gcggcttcct cgtacacgcg cgccctttcc gccatcaaga acgggaaatt cactgatgag
     2941 atcgtcccgg tcccggtacc gcagcgcaag ggagacccgg tgccgttcct ggtggacgag
     3001 gagcctggac gcgggaacgt gccgaagttg cccactctcc ggtccgcttt cgagaaaaac
     3061 ggcaccgtca ccgccgggaa cgcctcgacg atcaacgacg gggcggcggc cctggtcgtg
     3121 atgtcctcgg acgccgcgaa acgtctcggg gcgaagccgt tggcgcggat cgtcgggtac
     3181 gcgacggcat ccctcgagcc ggtctggttc accatagcgc cggtggacgc gatccggaca
     3241 cttcttcaga aaaccggcgt cgcgaaggag aaggtcgggc tcttcgagat caacgaggcg
     3301 tttgcggggg tggcgatcgc cgccattcgg gggctgtcgc tggatccgga gcgcgtgaac
     3361 gtgaatggcg gcgcggtggc gctggggcac cccgtcggct gctcgggggc gcggattctc
     3421 accacgctgc tgtacgccat ggccgaccgg ggggaacggt acggaatcgc ctccctttgc
     3481 atcggggggg gagaggctgt cgccgtgatg gtcgagaagc tatagataaa ctttcaagta
     3541 tgtatatcaa ctaaccgtta attattatgt tttctttggg ggaaacagtg agtgtttcga
     3601 agatcggcat cctcggggcc ggccagatgg ggagcgggat cgcgcaggtc tccctgatga
     3661 gcggcttgca ggtcgtcctg aacgatgtga ccgaggcggt cctcgcccgg tcccgcgcgg
     3721 gaatcgggaa aggtctcgac atcctcgtcc gcaaggaaaa gatctcgacg caggagaagg
     3781 aacgggcgct caacggcctc cagacgacga ccgatccctg cgacttcgcc tcctgcgatc
     3841 tcgtcgtgga agcggccacg gaacgggaag aactgaagct ctccctcttc cggaagctcg
     3901 acgaggtggt tccggcgggg cggatcctcg caacgagcac ctcctccatc tcgatcacga
     3961 agatcgccgc cgcgacgaag cgtccggcgc aggtgatcgg gatgcacttc atgaatccgg
     4021 tccctctcat gaaactcgtc gaggtgatcc cggggctgca gacgtcgccg gaaacgttcg
     4081 aagcgacgat gtcgttcgcc cgtgcgctcg gcaaggagc
//
LOCUS       DASVJL010000223        10233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_878940, whole genome shotgun sequence.
ACCESSION   DASVJL010000223 DASVJL010000000
VERSION     DASVJL010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10233
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878940"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VF357_04505"
     CDS             complement(<1..256)
                     /locus_tag="VF357_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523440.1"
                     /translation="MITHRVLYEIVSRALGPTLMAMERWRSRLRGRRGFRAEGPFGPP
                     LESTRRLKELTALHYLTGRFADGAVPVAWVTSGFPVEMLRP"
     gene            complement(253..1353)
                     /locus_tag="VF357_04510"
     CDS             complement(253..1353)
                     /locus_tag="VF357_04510"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEX7523441.1"
                     /translation="MMMRDATISARKKLGHKTVVVLPVHYPRELFTAMDVHTMELWGP
                     PGPPRGPDAGRIQAYACAVVRNALAFLASGGADAADAVLFPHTCDSIQGLATLAPDFG
                     GWGKPALRYLHPKGEDGPAARAFVRAELAHLADELARLTGRPLATSRLAEAIELHARI
                     DRLRGELLSRRAFLGMGDHALYRLLRRGEFLWPGDHLAELAGAARGLGEERVQRGVPL
                     MVTGYVPEPMTLFDSLASAGAFVAADDYAAVGRRVPLSPPAGTGDPLDTLAAKAFTMP
                     PCPTRGGDQDVRMEYLETLYRQSGAAGLVVHVQKFCEPELFDVPAIRRRFARIGAPLL
                     YLEGELETQLSGQFVTRLEAFVEMVHAGRKAP"
     gene            1478..2578
                     /locus_tag="VF357_04515"
     CDS             1478..2578
                     /locus_tag="VF357_04515"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEX7523442.1"
                     /translation="MKNVVRQTVFLFAVILLCAAGFAHSAGVDVKEGEWELSTESSMT
                     MGAMSMPPTAGKSTYCLTREDLVPKSEKDKDCKMVKQKVVGNTVSWRIECKKSEGEGE
                     ITYRGATYKGNFRMKMMEDGQSMTMNMKLAGKYLGPCAKGQKSGPTGETAKQVAKGQE
                     MAAQAKQQQAEMQAEQAANAKKGEAFFKRSVVPAEERGACAQEGFGRTPECEGKTGDL
                     NLKPGEYEITVERGSRIGTTYTPVETERKTVCLTDDGPVPEVLGAGRPPHVKRGKETI
                     TWTDAAGGRMTKGGILYLGNSLEGALTSTMDLGAGQGSLSVTKVAGRRIGDGNCIGGR
                     DYSAKKQGGDSAIGEKKILKDPVKGIRNLFGL"
     gene            2586..3419
                     /locus_tag="VF357_04520"
     CDS             2586..3419
                     /locus_tag="VF357_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7523443.1"
                     /translation="MPYPVRTALLLAFCLGIVAAAARVEAAGDPFGNVAAAYLVKVDG
                     REVWARNPGKRLPPGSLAKMMTALLVLELADLRGVATVSREASRETGTRLGLAPGDRM
                     TVLELLAATLIGSANDACHVLADHVAGSETKFVIRMNARARELGLSDTRFANACGHDA
                     PGMHSTARDLARLAETAMGNPVFAKMAGLKDGWISTVGAARAFSLENKNLLIGRYRGA
                     IGVKTGFTGSAGKCLVALAERRGKRVLLVLLNAPDRWWKAEEILDAAFAHGAGASSAR
                     P"
     gene            3416..4759
                     /locus_tag="VF357_04525"
     CDS             3416..4759
                     /locus_tag="VF357_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7523444.1"
                     /translation="MKRLSPFLLLAGAVAVVYLNSFRGVFQFDDYNVIVDNGGVHTWG
                     AFLAGLPRGIRPLLKFTYTLNWTSGLGLFGFHLVNVAFHAVNAVMLYFLASRIGGPSV
                     SRFTALLAALLFAVHPVQTEAVTYISGRSVSLMAFFYLGSLLAYLRGRERGSRYLLYL
                     ASPILFLLAVASKEVALTLPFALILCEAARRERDGWKETLRAQAVHWGILVALAVLFV
                     AHAGYGRLLEACFNIRGPAANLLTQAHGIGYLLSRLVMPHALNIDPDLPVFSGWAPGL
                     IPEALLLAALLAAGIFGLIIRFPAGFGIFWFFLHLAPTNSFIPRLDVANERQLYLASW
                     GLFLGVAAVVDLLREKGGARWLTAVAAVLVLALGVLTVSRNMVYRSEVALWEDAARKS
                     PGKARGWNNLGYAYQQAARFRDAEAAYLRALRIDPGYALARGNLRGLKARSDSVR"
     gene            complement(4851..5069)
                     /locus_tag="VF357_04530"
     CDS             complement(4851..5069)
                     /locus_tag="VF357_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523445.1"
                     /translation="MEGILFDIEMFASPVTVLLGLVLLAIGAGGILLGYMADEEASGK
                     RIFWAESPFTDVSEGETPVESVKYLRAA"
     gene            complement(5197..5469)
                     /locus_tag="VF357_04535"
     CDS             complement(5197..5469)
                     /locus_tag="VF357_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523446.1"
                     /translation="MKWIREILPRGNEAAFASGLLLLYGIVLFLSPDPEWTIWAGQMA
                     MGVLYVPDPPTMQGIAQGDAVRVMLGTGALGSLAVAALLALRIKTS"
     gene            5601..6269
                     /gene="folE"
                     /locus_tag="VF357_04540"
     CDS             5601..6269
                     /gene="folE"
                     /locus_tag="VF357_04540"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX7523447.1"
                     /translation="MSPDHEHETRECLSNMGANRRHVPADRMRQFEGYMAEILSAFGM
                     DPATPATVDTPRRFLQAMFDSTDGYEGDPKLITVFDTECRGGPDCRLSQVVEGPIQFF
                     SLCEHHALPFYGRAYVGYIAHEQIIGLSKLTRLVRLFAKRFTVQERIGQQIADSLDAM
                     LHPHGVAVYLDAHHLCTQMRGVRETAPLTRTTFWRGNYENDPSLRTEFFVACGLHRFE
                     PGSR"
     gene            6284..7132
                     /locus_tag="VF357_04545"
     CDS             6284..7132
                     /locus_tag="VF357_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7523448.1"
                     /translation="MTPLAPLESLLDGIRGDDIPLPAELATLYGPLRFPPRKGRPYVI
                     GNFVTTMDGVASLSAPGLAGGGPISGSNPHDRMVMGLLRSVADAVIVGAGTLRSVPNH
                     LWTAEHICPPLSDSYRQLRIASGKKASPLNVIVTARGEIDLDLPVFRSGAVKVLIVTN
                     PGGARRFRERDLPASVVIGEVGEGGAISARDVLSAVGRILSGEILLVEGGPRLMGDFF
                     AGKCLDELFLTIAPQVAGRDGSAERPGFVAGKRFAPERPLWGTLAGVKRAGSHLFLRY
                     SFATED"
     gene            7199..8455
                     /locus_tag="VF357_04550"
     CDS             7199..8455
                     /locus_tag="VF357_04550"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX7523449.1"
                     /translation="MDGLQHAAFDYFLRAVNPANGLVADTSREDSPASIAVVGFALAS
                     YPAAVERGWMTRADAVARSLAAMRFFHGSDQSGSPGATGYKGFYYHFLNLRTGDRVWR
                     SELSMIDTALLVAGALTASMFFRANSPDEIELRELVDAVYRRIDWRWAQGGTVTIRQG
                     WKPECGFLHYGWEGYSEAIVLYALALGSPTHPPEGDCYHAWTATYQWENLYGYDVLYA
                     GPLFVHQFSHAWIDLRGLRDRFMREKRDDYFENSRRSTYVQREYAQRNPREFAGYDDH
                     CWGLSAGDGPSDESPDVAKEPRRLFGYAARGVPYGPDDGTLSCSSVLASLPFAPEIVL
                     PAVRNMIGRYPEMLAGQQFASGFNPTLSGPDRRAWVSPGQYGLDQGIVMMMIENHRTQ
                     LIWRLMRDCPHVTTGLRRAGFRGGWL"
     gene            8482..9945
                     /locus_tag="VF357_04555"
     CDS             8482..9945
                     /locus_tag="VF357_04555"
                     /inference="COORDINATES: protein motif:HMM:NF004991.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEX7523450.1"
                     /translation="MQQDQQPSDGHHPQPADRYDLVVVGAGPAGLGAATAATALGARV
                     ALIERDVPGGTCLNTGCVPSKAIIRTSRLYAEMRDAERYGAQIPAEIRVDFPAVMQRM
                     RRIRARTVRPVSVHRLTAAGIDVFLGGARFTGTDTLAVDGTKLRFEKAMVATGSRPEI
                     PAIPGLVDADYLTNENVFDLTELPRRLLVVGGGPLGCELAQAFCRLGAKTIIAQYPPL
                     FLPKEERDAAQILSAAFARDGIEVRLNTRPINAHTEDGQKRVDLISDDYRSTVFVDRI
                     LAGAGRLPNVEGMDLEAAGVVYDPVNGIRVDDFLRTSNARIYAAGDACLEHKYTHTAD
                     AAARIVVQNALCRGRQRLSALVIPWCTYTDPEIAHVGLYVRQAYEQDLPVKTFTIPMH
                     DVTRAITDDEEVGFVKIHVKERTDRILGATIVARHAGEMINEITLAMVAGIGLRTLSR
                     VIHAYPTQAEAITKAADACSRTRSRPTIRSLLRRWLA"
BASE COUNT         1737 a         3341 c         3376 g         1779 t
ORIGIN      
        1 gcgggcgaag catctccacc gggaagccgc tggtgaccca ggcgaccggc accgcgccgt
       61 cggcgaagcg gcccgtgaga tagtggaggg ccgtcagctc cttgagcctg cgcgttgact
      121 cgagcggcgg tccgaacggg ccctccgcac gaaagcccct gcgcccgcgc aggcggctcc
      181 gccagcgttc catcgccatg agtgtcgggc cgagcgcacg cgacacgatt tcgtacagga
      241 cccggtgggt gatcacgggg ccttcctccc ggcgtgcacc atctccacga acgcttccag
      301 gcgggtgacg aactggccgg agagctgagt ctccagctcg ccctccaggt agagcagcgg
      361 cgcgccgatc cgggcgaagc ggcggcggat cgccgggaca tcgaagagtt ccggctcgca
      421 gaatttctgc acgtggacga cgagcccggc ggcccccgat tgccggtaga gggtctccag
      481 gtactccatc cggacgtcct ggtcgcctcc ccgggtcggg cacggcggca tcgtgaacgc
      541 cttggccgca agcgtgtcga gcggatcgcc ggtgcccgcc gggggggaca gcgggacgcg
      601 ccgtccgacc gcggcgtaat cgtctgcggc gacgaacgct ccggccgacg ccagggagtc
      661 gaagagggtc atcggctcgg gtacgtagcc ggtgaccatc agcggcacgc cgcgctggac
      721 acgctcctcg ccgagcccac gcgcggctcc cgcaagctcc gccaggtggt cgccgggcca
      781 caggaactcc ccgcggcgca ggagccggta cagcgcatgg tcccccatgc cgaggaacgc
      841 gcggcgggag aggagctccc cgcgaagccg gtcgatccgg gcgtgcagtt caatcgcctc
      901 cgcgagccgg gaggtcgcca gcggccggcc ggtcagccgc gcgagctcgt cggccaggtg
      961 agcaagctcg gcgcgaacga acgcccgggc ggcggggccg tcctcgccct tggggtgaag
     1021 atagcgcagg gcaggtttcc cccatccgcc gaagtcgggc gccagggtgg ccagcccctg
     1081 gatcgaatcg caggtgtgcg ggaagagaac ggcgtcggcg gcgtcggccc cgcccgaggc
     1141 gaggaacgcc aatgcgttgc ggacgaccgc gcaggcgtac gcctggatgc ggccggcgtc
     1201 cggaccgcga gggggacccg gcggtcccca cagctccatc gtgtggacgt ccatcgcggt
     1261 gaaaagctcc cgtggatagt ggaccggcag gacgaccacg gtcttgtggc cgagcttctt
     1321 ccgggcggag atcgtggcgt cgcgcatcat catctactct ggcatgtccc tccgcggtgg
     1381 gcaatgaaga ccctgacgaa catggatgcc accccgcaac gatatggtag gcttttcagg
     1441 gtgagtggct gggagtaacc atttaggagg atcgatcatg aagaacgtcg tccggcaaac
     1501 cgttttcctg ttcgccgtca ttctcctttg cgcggcggga ttcgcgcact cggcgggcgt
     1561 cgatgtgaag gaaggggagt gggagctctc caccgagtcg tccatgacga tgggggcgat
     1621 gtcgatgccg cccacggccg gcaaatcgac gtactgtctc acccgggaag atcttgttcc
     1681 gaagtcggaa aaggacaagg attgcaagat ggtgaaacag aaagtcgtcg ggaacacggt
     1741 ctcttggcgc atcgagtgca agaagagcga gggggaaggt gagatcacgt accggggggc
     1801 cacctacaag ggaaatttcc ggatgaagat gatggaagac gggcagtcga tgacgatgaa
     1861 catgaagctt gcgggtaaat atctcggccc ctgtgccaag ggtcagaagt cgggcccgac
     1921 cggggagacg gcgaagcagg tggccaaggg acaggagatg gcggcccagg cgaagcagca
     1981 gcaggcggag atgcaggcgg aacaggcggc caacgcgaaa aagggcgagg cgttcttcaa
     2041 gcggagcgtc gttccggcgg aggagcgcgg cgcgtgcgcc caggaggggt tcgggagaac
     2101 gccggaatgc gaggggaaga ccggcgatct caacctgaaa ccgggcgagt acgagatcac
     2161 cgtcgaacgt ggatcgcgca tcgggaccac ctacacgccg gtcgagacgg aacggaaaac
     2221 cgtgtgcctc accgacgatg ggccggtccc cgaggtactg ggcgcgggtc gtcctcccca
     2281 tgtgaaacgg ggcaaggaga cgatcacgtg gacggacgcc gccggtggac ggatgacgaa
     2341 gggcgggatc ctctacctgg ggaactccct cgaaggcgcc ctgacgagca cgatggacct
     2401 cggcgcaggc caaggatcct taagcgtcac gaaagtcgcc ggccgcagga tcggggatgg
     2461 aaattgcatc gggggccgcg actattcggc gaagaaacag ggaggggact ccgccatcgg
     2521 cgagaaaaag atattgaagg accccgtcaa gggaatccgg aatctcttcg gactctgacg
     2581 acggcgtgcc gtacccggtc cggacggcgc tgcttctggc cttctgcctc gggatcgtcg
     2641 cggcggccgc gagggtggag gcggccgggg atccgttcgg aaacgtcgcg gcggcgtacc
     2701 tcgtgaaggt ggacggcagg gaggtctggg cccggaatcc cgggaaacgc cttcctcccg
     2761 gcagcctcgc gaagatgatg acggccctcc tcgtcctcga gctggccgac cttcgaggcg
     2821 tggccacggt ttcccgggag gcgagccgcg agaccgggac gcgtctgggt cttgcgcccg
     2881 gcgaccggat gaccgtgctc gagctcctcg ccgccaccct catcggatcg gcgaacgacg
     2941 cctgccacgt cctcgcggac cacgttgccg ggagcgagac gaagttcgtg atacggatga
     3001 acgcccgtgc gagggaactg ggcctttccg atacccgctt cgcgaacgcc tgcgggcacg
     3061 acgcccccgg aatgcactcc acggcacggg acctcgctcg cctggcggaa acggcgatgg
     3121 ggaaccccgt gttcgcaaag atggcgggcc tcaaggacgg ctggatctcc acggtcggcg
     3181 cggcgagagc gttctccctc gagaacaaga acctgttgat cggccgctac cggggtgcga
     3241 tcggggtgaa gaccggcttc acggggtcgg ccggcaaatg cctcgtggcc ctcgcggagc
     3301 ggcggggaaa acgggttctt ctcgtactgc tgaacgcgcc cgaccggtgg tggaaggcgg
     3361 aggagatcct cgacgccgcc ttcgcccatg gagccggagc ctcctccgcc cgtccgtgaa
     3421 acgcctttcc ccgtttctgc tccttgcagg ggccgtcgcc gttgtctatc tcaactcctt
     3481 ccggggcgtc ttccagttcg acgactacaa cgtcatcgtc gataacggcg gcgtgcacac
     3541 ctggggcgct ttccttgccg gacttcctcg cggaatccgg ccgctcctga agttcaccta
     3601 caccctgaac tggacctcgg ggctcgggtt gttcggcttc cacctcgtga acgtggcgtt
     3661 ccacgcggtg aatgccgtca tgctctactt tctcgcgtcg aggatcggtg gcccatcggt
     3721 ctcccgcttc accgctctgc tcgccgcgct tctgttcgcc gtccatcccg tacagacgga
     3781 ggcggtgacg tacatcagcg ggcggtccgt ctcgctgatg gcgttcttct acctcggaag
     3841 cctcctcgcc tacctgcggg ggcgggagcg ggggagccga tatctcctct acttggcgtc
     3901 gccgatcctc tttcttctcg cggtcgcctc gaaagaggtg gccctcaccc ttccgttcgc
     3961 gctgatcctt tgcgaagcgg cccggcggga gagggacgga tggaaggaga cgcttcgcgc
     4021 ccaggcggtc cactggggta ttctcgtggc tctcgccgtt ctcttcgtcg cccacgccgg
     4081 ctatgggcga ttgctcgagg cgtgcttcaa catccgggga ccggcggcca acctgctcac
     4141 gcaggcccac gggatcggat atcttctgtc tcgtctggtg atgccgcacg ccctgaacat
     4201 cgaccccgac ctgcccgtct tctccgggtg ggcgcctggc ctgattccgg aagccctcct
     4261 tcttgcggcg ctccttgccg cggggatctt cgggctgatc atacggttcc cggcggggtt
     4321 cggcatcttc tggttctttc tacatctcgc tccgacgaac tccttcatcc cccgtctcga
     4381 cgtggcgaac gaacgccaac tgtacctcgc ctcctgggga ctctttctgg gcgtggcggc
     4441 ggtggtggat cttctgcggg agaagggggg cgcgcggtgg ctgacggcgg tcgccgccgt
     4501 cctggttctc gctctcggcg tgctcaccgt gtcccggaac atggtatacc gcagcgaggt
     4561 cgcattgtgg gaggacgcgg cgcggaaatc cccggggaag gcgaggggat ggaacaatct
     4621 cgggtacgca taccagcagg cggcccgctt ccgcgatgcc gaggcggcgt atctgcgggc
     4681 gctccgcatc gatcccgggt atgcgctcgc ccggggaaac ctcaggggac tcaaggcgag
     4741 atcggattcc gttcgctgac gaaattgtgt cgcgggtgcg ccggcttcaa tcgaaaaaag
     4801 gggattgcgc tcttccgcaa tcccccggcc gggacggttc cgtgacggca tcacgccgcc
     4861 cggagatact tcacgctttc caccggcgtc tcgccttcgc tcacgtcggt gaacggggat
     4921 tccgcccaga aaatccgttt cccgctcgcc tcctcgtcgg ccatgtagcc caggagaata
     4981 ccgcctgctc cgatcgcgag cagcaccagc cccaacagta cggtgaccgg cgatgcgaac
     5041 atctcgatgt caaacaggat gccttccatc gtgctcacct ccttctcttt ccatctgtcc
     5101 ccacgatact tccgcgaggg aaacggttct gtgcgacctt tcacaatgcc ccatccatct
     5161 cctccgccgg aaggggccga tatgcgagac gccccatcat gacgttttga tccgcagcgc
     5221 caggagtgcc gccaccgcga ggcttcccaa cgcccctgtc cccaacatga cccgcacggc
     5281 atcgccttgc gcgatcccct gcatcgtcgg cggatcaggt acgtacagaa cgcccatcgc
     5341 catctgtccc gcccatatcg tccactccgg gtctggggag agaaagagca cgatcccgta
     5401 gagcaggagc agccccgatg cgaacgccgc ctcgtttccc cttgggagga tctcccggat
     5461 ccacttcatg gcggccacct ccttccttct gaccgttaga taacatggag gggagagaac
     5521 gtttgtgaaa ggtcaaacag atccgatatt ttccttggag ggcgttaaaa taagattacc
     5581 tttcgttcca aggagcgccg atgagtccgg accatgagca tgagacccgg gaatgtttgt
     5641 cgaacatggg cgcgaaccgc cgccatgttc cggcggatcg gatgcggcag ttcgaaggct
     5701 acatggcgga aatcctctcg gccttcggga tggatccggc cacgccggcc acggttgata
     5761 ccccgcgacg gtttctccag gcgatgttcg acagcaccga cgggtacgag ggggatccga
     5821 aactgatcac ggtgttcgat accgagtgcc gcggagggcc ggactgccgg ctcagccagg
     5881 ttgtggaagg gccgattcag ttcttcagcc tctgcgaaca ccacgcgctt cctttttacg
     5941 gccgcgcgta cgtagggtac atcgcccacg agcagatcat cggcctctcc aagctgacga
     6001 ggctggtgcg cctgttcgcg aaacggttca ccgtgcaaga gcggatcggc cagcagatcg
     6061 ccgactccct ggacgccatg cttcatccgc acggcgttgc ggtgtacctg gacgcgcacc
     6121 acctctgcac ccagatgcgc ggggttcggg agaccgctcc gctcacgcgc acgacgttct
     6181 ggcgcgggaa ttacgaaaac gacccgtcgc tgcggaccga atttttcgtc gcatgcggcc
     6241 tgcatcgctt cgagcccgga tcccggtaga aagggggcgt gcgatgactc cccttgcgcc
     6301 cctggaatcc cttcttgatg ggatccgcgg agacgatatc cctctgcccg ccgagctggc
     6361 gacgctctac ggccccctcc gatttcctcc acggaaaggc cgtccgtacg tcatcggcaa
     6421 cttcgtcacc acgatggacg gcgttgcatc cctctccgct cccggtttgg cgggaggtgg
     6481 ccccatcagt ggttccaacc ctcacgaccg gatggtcatg ggactgttgc gatccgtcgc
     6541 ggatgccgtg atcgtcggcg ccgggacact tcgctccgtc ccgaaccacc tctggaccgc
     6601 ggagcatatc tgtccgccgc tatccgactc ataccggcag ctgcggatcg catcagggaa
     6661 aaaggcctcc ccgttgaacg tcatcgtcac cgcgaggggt gagatcgacc tggaccttcc
     6721 cgtcttccgg tccggggcgg tgaaggtgct gatcgttacg aatccggggg gcgcccgccg
     6781 gtttcgcgaa cgcgaccttc ccgcctccgt cgtcatcggg gaagtagggg aggggggcgc
     6841 gatcagcgcg cgggatgttc tttccgccgt cggccgtatc ctctccggcg aaatcctcct
     6901 tgtcgaaggc ggaccgcggc tgatgggaga tttcttcgcc gggaaatgcc tggatgagct
     6961 ttttttgacg atcgctccgc aggttgccgg gcgggacggg tccgccgagc gtcccggatt
     7021 cgtcgccggg aagcgatttg caccagagcg tcccctgtgg ggaacgcttg ccggcgtgaa
     7081 acgtgccggc agccatctgt ttttaaggta ttcgtttgcg acggaggatt gatccccggt
     7141 gggacccttt cgcgcggttc ccgtggctga ggtgacagcg cccccggacg gggtcctgtt
     7201 ggatggactg cagcacgcgg cgttcgacta cttcctgcgg gccgtgaatc cggcgaacgg
     7261 tctcgtcgcc gacacctcgc gagaggattc acccgcgagc atcgcggtgg tgggctttgc
     7321 gctggcgagc tatcccgctg ccgtggagcg cggttggatg acgcgggccg acgcggtcgc
     7381 gcgcagcctc gccgcgatgc gcttcttcca cggcagcgat cagagcggca gccccggggc
     7441 gaccggttac aaaggtttct actaccactt cctgaacctg cgcaccggcg accgcgtctg
     7501 gcgctcggag ctatcgatga tcgacaccgc gctgctcgtc gccggcgcgc tgactgcgag
     7561 catgttcttc cgcgcgaaca gtcccgacga aatcgagctg cgcgagctgg tcgatgcggt
     7621 gtaccggcgc atcgactggc gctgggcgca aggcggtacg gtcacgatcc gacagggttg
     7681 gaagcccgag tgcggatttt tgcactatgg atgggaaggc tacagcgagg caatcgtgct
     7741 atacgcgctc gctctgggct cgccgactca cccgcccgaa ggcgactgct accacgcatg
     7801 gaccgcgaca taccagtggg agaacctgta cggttacgat gtcctgtacg cggggccact
     7861 cttcgttcat cagttctcgc acgcgtggat cgatcttcgt ggcctccggg atcgcttcat
     7921 gcgcgagaag cgcgacgact acttcgagaa cagccggcgc tcgacctacg tccagcgcga
     7981 gtacgcgcag cgtaacccac gcgagttcgc cgggtacgac gaccactgct ggggcctctc
     8041 ggcaggtgat ggtccgagcg acgagtcgcc cgacgtggcc aaagagccgc gccgtctgtt
     8101 cggctacgcc gcccgggggg tgccgtacgg accggacgac ggaaccctgt cctgttcctc
     8161 ggtgctggcg tcgttgccgt ttgcccccga gatcgtcctg cctgcggtac gcaacatgat
     8221 cgggcgctat cccgagatgc tggccggtca gcagttcgcc agcggcttca atccgactct
     8281 ctccggcccg gacaggcgcg cgtgggtctc gcctggccag tatggactcg accaaggcat
     8341 cgtgatgatg atgatcgaaa accaccgcac gcagctgatc tggcgcctga tgcgggattg
     8401 cccccatgtc accaccggac tgcggcgggc cgggtttcgc ggcggttggc tgtaagaccc
     8461 tttcaaggac ggatcccgac gatgcagcaa gaccagcaac cctccgacgg ccaccatccg
     8521 cagcccgcgg accgatacga cctggtggtc gtcggcgccg ggccggcggg gctcggtgcg
     8581 gcgaccgcgg cgaccgccct gggcgccagg gtcgcgctca tcgagcgcga tgtgcccggc
     8641 ggcacttgcc tgaataccgg atgcgtaccg tcaaaggcca tcattcgcac gtcgcggctg
     8701 tacgccgaga tgcgtgacgc cgagcgctat ggcgcgcaga tcccggccga gatccgcgtt
     8761 gactttcctg ccgtgatgca gcggatgcgc cgaatccgcg ctcggaccgt ccgacccgtt
     8821 tcggtccacc ggttgaccgc ggccggcatc gatgtgttct tgggcggcgc tcgcttcacg
     8881 ggaaccgata cgctggccgt ggacggcacg aaactgcgct tcgagaaggc gatggtcgcc
     8941 accggttcgc gtcccgagat ccccgccatc ccgggtctgg tcgacgcgga ctacctgacc
     9001 aacgagaacg tgttcgacct gaccgagttg ccgcgccgtc tgttggtggt cggcggcggt
     9061 cccctcggct gtgagctggc gcaggcgttc tgtcgactgg gcgccaagac aatcatcgcc
     9121 cagtaccctc cgctgttcct gcccaaggaa gagcgcgatg ccgcgcagat cctgtccgct
     9181 gcctttgcgc gcgacggcat cgaggtgcgg ttgaatacca ggcccatcaa cgcccacacc
     9241 gaagatggcc agaagcgggt cgacctgatc agtgacgact atcggagcac ggtcttcgtc
     9301 gaccggatcc tcgccggcgc tggccggctg ccgaacgtcg aaggcatgga tctggaagcc
     9361 gcgggggtgg tctacgatcc cgtgaacggc atccgggtcg acgacttcct acggacgagc
     9421 aatgcgcgca tctacgccgc cggcgacgcc tgcctggagc acaagtacac ccacaccgcc
     9481 gacgccgccg cgcgcatcgt ggtccagaac gcgctgtgtc gtggccgcca gcgactgagc
     9541 gccctggtga ttccctggtg cacctacacc gatccggaga tcgcccatgt cggcctctac
     9601 gtccggcagg cttacgaaca ggacctcccc gtgaagacct tcaccatccc gatgcacgac
     9661 gtgacgcgcg cgatcaccga cgatgaggag gtcggcttcg tgaagatcca cgtcaaggag
     9721 aggaccgatc gcatcctcgg ggcgacgatc gtcgcccggc acgccggcga gatgatcaac
     9781 gagatcaccc tggccatggt cgcggggatc ggtctgcgga ctctctcgcg ggtcatccac
     9841 gcctatccga cccaggcgga agcgatcacg aaggccgcgg acgcctgcag ccgtacacgt
     9901 tcgaggccga ccatccgatc gctgctgcga cgctggctgg cgtgatcatg atgccgtttt
     9961 catctttcgg cttcggcagg gaagttgtgc aaatcccccc cgaagatgag agtgcttaaa
    10021 gggcccgtgg aaccgtcccg gccgggggaa tgcgctcttc cgcaatcccc cggccgggat
    10081 gaccggcgat gcgaacatct cgatgtcaaa caggatgcct tccatcgtgc tcacctcctt
    10141 ctctttccat ctgtccccac gatactcccg ggagggaaac ggttctgtgc gacctttcac
    10201 aatgccctcg gcgcccggga acgaaactcc aag
//
LOCUS       DASVJL010000224         7325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_879010, whole genome shotgun sequence.
ACCESSION   DASVJL010000224 DASVJL010000000
VERSION     DASVJL010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879010"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VF357_04560"
     CDS             <1..277
                     /locus_tag="VF357_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX7523451.1"
                     /translation="LVVALVVTAAIHAVGILLVTALLVIPAAAARNLARGAAAALWVS
                     VGVSALSGFAGIVASWYLDTATGATVVLAAATCFAVTALYRVTRPRE"
     gene            375..947
                     /locus_tag="VF357_04565"
     CDS             375..947
                     /locus_tag="VF357_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX7523452.1"
                     /translation="MKKLKGSRTEHNLMAAFAGESQARNRYTFYAGIASKEGHEGVAL
                     IFLETAANEMMHAKRYFELLEGGDVEITAGYPSKIGSTAVNLEAAAAGEHEEWTNLYP
                     TFGKIAEEEGFAAPAAIFRRVAEVEVEHEKRYLRLLDRVKNGTLYKREKPIRWKCEKC
                     GRIHEGTEAPERCPTCGHPQGWFIPAEANY"
     gene            complement(1039..1341)
                     /locus_tag="VF357_04570"
     CDS             complement(1039..1341)
                     /locus_tag="VF357_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX7523453.1"
                     /translation="MSDERCCNGSGGFLLAFLAGGLVGAGLALLYAPVSGREARERIG
                     GLAGDLKKKTDEWTSDAKKKVEGFIDEERSVIKAAYDAGREAMVREKARFENPNPE"
     gene            complement(1338..1736)
                     /locus_tag="VF357_04575"
     CDS             complement(1338..1736)
                     /locus_tag="VF357_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523454.1"
                     /translation="MDAQTIFFIALAVAVVALVVVVSVALLSIRRNVDRIAQRFDESL
                     RQFEMTAEDVRKTNAAVREILSDIDRGVSNVTHFTEGVRALRGTVDVATNVFAHAVSP
                     ALVTAASVLAGLKAATYHILDRFVRKEERK"
     gene            1895..2491
                     /locus_tag="VF357_04580"
     CDS             1895..2491
                     /locus_tag="VF357_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7523455.1"
                     /translation="MERPGGKGQPADLLVEGFRKGAPGAFEAIVRAHQDRVYSFCARM
                     LSDREDALDAAQEVFLSAWRNLSEFRGEAALSTWLLRIAANRCLNRIRRRKSLSGREA
                     PWPEPSGEVDEGFVFQPPGKETDRPDRLAETCEMGEILTEALSRLDPGSRWMVLLSDV
                     EGFSYEEIAALADVPVGTVKSRLHRARMAMRRLLSPVT"
     gene            2509..3504
                     /locus_tag="VF357_04585"
     CDS             2509..3504
                     /locus_tag="VF357_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7523456.1"
                     /translation="MDCMQARNLLSEYQDGTLDAAAAAALDAHLRGCGECTGRVGSLL
                     AVRQLLRGLPPDPAPPELLARVLAAVEAEDRGAGADSSPEGTDASRPFLSRFRIPLEA
                     AAVVLLFASAYWYQRTPTPVAHPPFGLSSGMPSETGKVPSSRPMAAKEDAAKSSPSGI
                     RLTRGNPKTAKEDAPAAPRPRPWTAASLPSVPALRASTDSEPIVPVAPSSGPMVDPAP
                     AGAPAFDMRRMPYGRDIAVDVNPESLEGAEDRIAEATLRLGGIVERIEREREPARKGA
                     EGTIRVILPEAEAAGFLEELRRIGNVPQENLSAAIDLPAGPRPGTVAYAVHIRVR"
     gene            complement(3547..4059)
                     /locus_tag="VF357_04590"
     CDS             complement(3547..4059)
                     /locus_tag="VF357_04590"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7523457.1"
                     /translation="MHRDPSKQEYLPHSSGCFLCGEENRSGVRARFFVEGNEVLGRVI
                     LPLHLNGYKNVAHGGVVSALLDETMGWAATVFGKTHPMYVTGELTVKFLSPVPVGAEI
                     EVRSRLVGDAGRLAYCEGEIHCDGKVCARAKGKFVPMSPEGTAEVIPYLRFEGCRRFR
                     AIFDAYRGGK"
     gene            complement(4146..6806)
                     /gene="ppdK"
                     /locus_tag="VF357_04595"
     CDS             complement(4146..6806)
                     /gene="ppdK"
                     /locus_tag="VF357_04595"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX7523458.1"
                     /translation="MAKRAKKYVYYFGNGKADGTGKMKDLLGGKGAGLANMTNLKIPV
                     PAGFTITTEACNTYFESRKKYPPGMWEQVLANLKKVETAMGMKFGDAKNPLLVSVRSG
                     SKFSMPGMMDTILNLGLNDTTLKALADKAGDRFAFDTYRRLITMFGSTAMGINRQLFE
                     KALEGLKERKGVRLDTELATEDLRELVGIFKGIYEKETGKAFPADPLQQLKLAINAVF
                     SSWFSDRAVTYRKLNNIANDLGTACNVQAMVFGNMGENSGTGVGFTRDPSTGQKKFFA
                     EYLTNAQGEDVVAGIRTPLHIDELKKKQPAVYNELEKIYRKLEYHYKDMLDIEFTVQD
                     GKLYMLQTRVGKRTAAASLKIAIDMVQEKLIDKKTAVMRIDPVQIEWLMHPSFDPKAS
                     VQVIGKGLPASPGAAVGKVVFSAEDAEAWAAKGENVVLVRTETSPEDIGGMHAAQGIL
                     TARGGMTSHAAVVARGMGKCCVAGCNALHIDEGTKTLILGSRVLKEGDFISLNGTTGE
                     VILGLVPLVPADLSGDFHLILKWADEIRELGIRANADTPADARMALKFGAEGIGLVRT
                     EHMFFEGDRVQAVREMILADDTEGRKKALAKILPMQREDFIGIFKVMNGLPVTIRLLD
                     PPLHEFLPKTQEELEDIARDLGVPIQKLQARNKALHEFNPMLGHRGCRLGITYPEIFE
                     MQVQAIMEAACHVVKQKVKAVPEIMIPLIADVNELRVMREMTVRIAEEVQKRTKVRVR
                     YEVGTMIEIARAALLAHEIAPEADFYSFGTNDLTQTTYGLSRDDAGRFLPYYVEKGIF
                     EHDPFITIDRKGVGMLMKMAIDNSRKIKKGMKMGICGEQVDPLSVEFCYEIGLTYVSG
                     SPYRLPVARLAAAQATLKKKMKKGLSKASM"
     gene            complement(7076..>7325)
                     /locus_tag="VF357_04600"
     CDS             complement(7076..>7325)
                     /locus_tag="VF357_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523459.1"
                     /translation="MDKYPIIITIGAAVLGKVAGEMVITDPWIHKTFLPSRATEIAVM
                     IFFTVGVVVVGKLLLRRKAGKEPELSVVPEPASLTEKK"
BASE COUNT         1243 a         2428 c         2303 g         1351 t
ORIGIN      
        1 gctggtcgtc gccctcgtgg tgaccgcggc gatccacgcc gtgggaatcc tcctggtcac
       61 cgccctgctc gtcatccccg ccgccgccgc ccgcaacctc gcccgggggg ccgccgcggc
      121 cctctgggtc tccgtgggag tctccgcgct gtcgggattt gccgggatcg tcgcctcctg
      181 gtatctcgac accgccaccg gtgcgaccgt cgtcctggcc gccgcgacct gtttcgccgt
      241 caccgccctg taccgggtca cccgcccgag ggaataatac ggccgtcgat catttccgtt
      301 gcgccatcgc aggacatctc tctataattg tcgacaatcg ttccctttaa cattccaacc
      361 ggatcggagg acacatgaaa aagctgaagg gatcgcgcac ggagcacaac ctgatggccg
      421 cgttcgctgg cgagtcgcag gcgcggaacc ggtacacctt ctacgcgggg atcgcatcga
      481 aggaggggca cgagggagtc gccttgatct tcctcgagac ggcggcaaac gagatgatgc
      541 acgccaagcg gtacttcgaa cttctcgagg ggggggacgt ggagatcacg gcggggtacc
      601 cgagcaagat cgggagcacc gccgtgaacc tcgaggccgc cgccgccggt gagcacgagg
      661 agtggacgaa cctctacccg accttcggga aaatcgccga ggaggaaggg ttcgccgccc
      721 ccgccgcgat ctttcgccgg gtcgccgagg tcgaggtgga gcacgagaag cggtacctgc
      781 ggctcctcga ccgcgtgaag aacggcacgc tctacaagcg ggagaagccg atccggtgga
      841 agtgcgagaa atgcggccgg atccacgagg ggaccgaggc gccggagcgc tgccccacct
      901 gcggacatcc gcaggggtgg ttcatccccg ccgaggcgaa ctactgatcc atggatccag
      961 ggcggcaacc cgggtgcgcc ggggttgccg cccttgcttc ctccaagggg agccccggcc
     1021 cccgggaact gccgtcgctc actccgggtt cgggttctcg aagcgggcct tctcccgcac
     1081 cattgcctcg cggccggcat cgtacgccgc cttgatcacg gaccgctcct cgtcgatgaa
     1141 cccctccacc ttcttcttcg cgtcgctggt ccactcatcg gtcttcttct tgagatcccc
     1201 cgccagcccg ccgatccgct cccgggcctc gcgtccggaa acgggggcat acagcagggc
     1261 aagccccgcg ccgaccaacc cgccggcaag gaacgccagc aggaatccgc cggaaccgtt
     1321 acagcacctt tcgtcgctca tttcctctcc tcctttcgga cgaaccgatc gagaatgtgg
     1381 tacgtcgccg ccttgaggcc cgcgaggacc gaagcggcgg tcaccagggc gggggacacc
     1441 gcgtgggcga acacattcgt cgccacgtcc accgtcccgc gaagagcgcg gaccccctcg
     1501 gtgaagtgcg tcacgttgga taccccccgg tcgatgtcgg aaaggatttc ccggacggcc
     1561 gcgttcgtct tgcgcacgtc ctcggcggtc atttcgaact gccggagcga ctcgtcgaac
     1621 cgctgcgcga tccgatccac gttccttcgg atcgacagaa gcgcgaccga cacgaccacc
     1681 acgagcgcaa ccacggcgac cgcgagggcg atgaagaaga tcgtctgtgc gtccaagggc
     1741 tacctcccgt gcagatcggt gggatcgggg acgccccccg acccgtctct aaaatacctt
     1801 aaaacggtag gggaccgcaa tcggtcctgc ggaggagtaa aattggagga gggaacagga
     1861 tcgcgagggc gcgtgtcgaa caaggaggat gcatttggag aggccgggag gaaagggaca
     1921 acccgccgac ctgctcgtgg aggggttccg gaaaggggcg ccgggagcct tcgaagcgat
     1981 cgttcgggcg caccaggatc gggtctactc cttctgcgcc cggatgctct cggaccggga
     2041 ggacgcgctc gacgcggcgc aggaagtatt tctttccgcc tggcgaaacc tctcggaatt
     2101 ccggggggaa gcggccttgt cgacgtggct gttgcggatc gccgcgaatc gctgcctgaa
     2161 ccggatccgg cgccgcaaat ccctttcggg gcgcgaggct ccctggccgg agccgtccgg
     2221 ggaggtggat gaaggattcg tctttcagcc gccgggcaag gagaccgacc ggccggaccg
     2281 gctcgcggag acttgcgaga tgggagagat cctgacggaa gcgctgtcac ggctcgatcc
     2341 gggctcccgg tggatggtgc tcctctcgga tgtggaggga ttctcgtacg aggaaatcgc
     2401 cgccctcgcg gatgtcccgg tgggtaccgt aaagtcgcgg ctgcatcgcg cgcggatggc
     2461 gatgcgtcgg ctgctttcgc cggtgacgtg aagagaagaa tggacacgat ggattgcatg
     2521 caagcgcgaa acctgctgtc cgagtaccag gacggcaccc tggacgccgc agccgcggcg
     2581 gcgctggacg cccaccttcg aggatgcggc gaatgcaccg gccgcgtcgg ttccctcctc
     2641 gcggtgcggc aactcttgcg ggggctgcct cccgatccgg cgcccccgga actgctcgca
     2701 agggtcctgg cggccgtcga ggcggaggac cggggcgccg gtgcggattc ctcgcccgaa
     2761 gggacggatg cgtcgaggcc atttctttcc cggttccgga tccccctcga ggcggccgcg
     2821 gtcgtactgt tgttcgcctc cgcctactgg taccagcgaa ctcctacgcc tgtcgcccac
     2881 cctccgttcg gcctctcctc cggcatgccc tccgaaaccg gcaaggtacc ctcttcccgc
     2941 ccaatggcgg cgaaggagga cgcggccaag agctcgccat ccgggatccg acttacacgt
     3001 gggaacccga agacggcaaa ggaggatgcc cctgccgctc cgagaccccg gccctggacc
     3061 gcggcgagcc tcccgtcggt gccggcgctg cgtgcgagca cggactccga gccgatcgta
     3121 ccggtcgccc cgtcctccgg accgatggtc gatcccgctc ccgcgggagc accggcgttc
     3181 gacatgaggc ggatgccgta tggacgagac atcgccgtgg acgtgaaccc ggagagcctt
     3241 gaaggggcgg aggatcggat cgccgaggcg acgttgcgtt tgggcggcat cgtcgaacgg
     3301 atcgaacggg aacgcgaacc ggcgaggaaa ggcgcggagg gcacgatccg ggtgatcctg
     3361 ccggaagccg aggcggccgg gttcctcgag gaactgcggc ggatcggaaa tgtgccgcaa
     3421 gagaacctgt cggcggcgat cgaccttccc gcgggcccgc gccccggcac cgtcgcctac
     3481 gcggtccaca tccgcgtcag ataaaatcga ccccccggga agacgcgacg attccgggca
     3541 aatccatcac ttcccccccc gatatgcgtc gaagatcgcc cggaagcggc ggcatccctc
     3601 gaaccggagg tacgggatca cttccgccgt cccctccggg ctcatcggga cgaactttcc
     3661 cttggcgcgg gcgcacacct tcccgtcgca atggatctcc ccctcgcagt aggcgagcct
     3721 ccccgcatcc ccgacgagcc ggctgcggac ctcgatctcg gcaccgaccg gcacggggga
     3781 aaggaacttc accgtaagct cgcccgtgac gtacatgggg tgcgttttgc cgaacacggt
     3841 ggcggcccac cccatcgtct cgtcgaggag cgcggagacg accccgccgt gggcgacgtt
     3901 cttgtacccg ttgagatgga gggggaggat caccctgcca agaacctcgt ttccctccac
     3961 gaagaaccgc gcgcgcacgc cggagcggtt ctcttccccg cagagaaaac atcccgagga
     4021 gtgcggcagg tactcctgct tggacgggtc ccgatgcatc ccgtcatctt atcagaggtg
     4081 cgcccgcagg aaacggaaga gggctccctc ccatgcaggg agggagccct ccggttgcga
     4141 ggacgttaca tcgatgcctt ggacagcccc tttttcatct ttttcttgag cgtcgcctgc
     4201 gccgcggcaa ggcgcgcgac cggcagccgg tacggcgagc cgctcacgta ggtgagcccg
     4261 atctcatagc agaattcgac cgataacgga tccacctgtt ccccgcagat ccccatcttc
     4321 atccccttct tgatcttccg gctgttgtcg atcgccatct tcatcagcat gccgactccc
     4381 ttgcggtcga tggtgatgaa cgggtcgtgc tcgaagatcc ccttctcgac gtagtagggc
     4441 aggaaccttc ccgcgtcgtc ccgggaaagg ccataggtcg tctgggtcag gtcgttggtg
     4501 ccgaaggagt agaaatcggc ctccggcgcg atctcgtggg ccagcagcgc cgcccgtgcg
     4561 atctcgatca tggttcccac ttcgtaccgg acacggacct tggtgcgctt ctggacctct
     4621 tcggcgatcc gaacggtcat ctcgcgcatc acgcgcagct cgttcacgtc ggcgatgaga
     4681 gggatcatga tctcggggac ggctttcact ttctgtttca ccacgtgaca ggcggcttcc
     4741 atgatggcct ggacctgcat ctcgaagatt tccggatagg tgatgccgag acggcatccc
     4801 ctgtggccca gcatggggtt gaattcatgc agcgccttgt tccgcgcctg cagcttctgg
     4861 atcggcacgc ccaggtcgcg ggcgatatct tcgagctcct cctgggtctt cgggaggaac
     4921 tcgtgcagcg gcgggtcgag gagccggatg gtcaccggca gcccgttcat caccttgaag
     4981 atgccgatga aatcctcccg ctgcatcggg aggatcttcg cgagggcctt tttgcgccct
     5041 tccgtatcgt cggccaggat catctcgcgc accgcctgca cccggtcgcc ctcgaagaac
     5101 atgtgctccg tgcggacgag cccgatcccc tcggcgccga acttgagcgc catgcgggcg
     5161 tcggccggcg tgtcggcatt ggcacggatt ccgagctccc ggatctcgtc ggcccacttc
     5221 agaatcaggt ggaaatcgcc gctgagatcg gcgggaacga gggggaccag cccgaggatg
     5281 acttcccccg tggtgccgtt cagggagatg aaatcgccct ccttcaggac gcgcgatccg
     5341 aggatcaacg tcttcgttcc ctcatcgatg tggagcgcgt tgcaccccgc gacgcagcat
     5401 ttgcccatgc ccctcgccac cacggccgcg tgggacgtca tcccgccccg ggccgtgagg
     5461 atcccctggg ccgcgtgcat tccgccgata tcctccgggg aggtctcggt ccgtaccagg
     5521 accacgttct ctcccttggc ggcccacgct tccgcgtctt ccgccgagaa aacgactttt
     5581 ccgaccgcgg cgccgggcga cgccggcagt cctttcccga tgacctgtac ggacgccttc
     5641 ggatcgaagg agggatgcat gagccattcg atctgcacgg ggtcgattct catgaccgcc
     5701 gtcttcttgt cgatcagctt ctcctggacc atgtcgatgg cgattttcag ggacgcggcg
     5761 gcggtccgtt tcccgacgcg ggtctgaagc atgtagagct ttccatcctg gacggtgaat
     5821 tcgatgtcca gcatgtcttt atagtggtat tccagtttcc ggtagatctt ttcgagctcg
     5881 ttgtacacgg cgggctgctt cttcttcagc tcgtcgatgt gaaggggggt ccggatgccg
     5941 gccacgacgt cctcgccctg cgcgttcgtg agatattcgg caaagaactt cttctggccg
     6001 gtggaggggt ccctggtgaa accgacgccg gttcccgaat tctcgcccat gttcccgaag
     6061 accatcgcct ggacgttgca cgcggtcccg agatcgttcg cgatgttatt gagcttccgg
     6121 tacgtgaccg cccggtcgga gaaccaggag ctgaacacgg cgttgatggc cagcttgagc
     6181 tgctggagcg ggtccgccgg aaaagccttt cccgtctcct tctcgtagat gcctttgaag
     6241 attccgacca gttcccgaag atcttccgtc gccagctccg tatcgagacg gacgcccttc
     6301 ctttccttca acccttccag cgccttttcg aacaactggc ggttgatccc catggcggtg
     6361 cttccgaaca tggtgatcag ccgccggtag gtgtcgaacg cgaaccggtc gccggccttg
     6421 tcggcgaggg ccttgagggt cgtgtcgttg agcccaaggt tgagaatcgt gtccatcatc
     6481 ccgggcatgg aaaatttcga gccggaacgg acggacacga ggagggggtt tttcgcgtcc
     6541 ccgaacttca tgcccatcgc cgtctccacc ttcttcaggt tggcgagcac ctgttcccac
     6601 atgccggggg gatacttctt cctgctctcg aagtacgtgt tgcacgcctc cgtggtgatg
     6661 gtgaaccctg cgggcaccgg gatcttcagg tttgtcatgt tggccagccc cgcccctttc
     6721 ccgcccagca ggtccttcat cttcccggtt ccgtccgcct tcccgttccc gaaataataa
     6781 acatactttt tcgcgcgttt cgccatcaga gacctccgaa aggcgtggtt gatttgcaac
     6841 tgcccccgaa aagacaaacc cacgggcggg atgcggttcc ccgtcgctga ccggccatca
     6901 ttataatgaa ataattgccc ggaattcaag gcgttcacgc cctgaagaaa gaaaggcccc
     6961 gacccggtcg tgccgcctgc cgctccctgg cgtcatctcc cgaaaggtcg gggcctgtcc
     7021 atgccgtgcg gatcgatcgt tacaacgcgc gcgccaccgt cacggtggcc atgcgttact
     7081 tcttttcggt aaggctggca ggttccggaa ccaccgacag ctccggctcc ttgccggctt
     7141 tccgccgcaa caggagtttt cccaccacga cgaccccgac ggtgaagaag atcatcacgg
     7201 cgatttccgt ggcgcgcgac gggaggaacg tcttgtggat ccagggatcc gtgatgacca
     7261 tctcgccggc caccttgccc aggaccgcgg ctccgatcgt gatgatgatc gggtatttgt
     7321 ccatc
//
LOCUS       DASVJL010000225         3825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1160687, whole genome shotgun sequence.
ACCESSION   DASVJL010000225 DASVJL010000000
VERSION     DASVJL010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160687"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VF357_04605"
     CDS             <1..379
                     /locus_tag="VF357_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX7523460.1"
                     /translation="QPFPGPGLAVRIIGPVTEERLRILREADLIVDAEIRAAGLYESI
                     WQSFAVLLPIKTVGVMGDFRTYENVAAVRAVHSQDGMTADWVRLPYDVLQRISTRIIN
                     GVKGINRVAYDISSKPPGTIEWE"
     gene            376..>3825
                     /gene="dnaE"
                     /locus_tag="VF357_04610"
     CDS             376..>3825
                     /gene="dnaE"
                     /locus_tag="VF357_04610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX7523461.1"
                     /translation="MKSFIHLHLHSEYSLLDGTIQFKPLIERVKKLKMPAVAVTDHGG
                     MMGTIGFYEEALKGGVKPILGTEIYVAPESRTERKVAPSGEYAYHLILLAENEIGYGN
                     LLRLSSLAHTEGFYYKPRVDKELLRRYSEGLIATSACLQGEIPFAMGTEGEAKALEVL
                     EEYKSIFTDGRFYIEIQDNGLPDQAKMNPLLIKLARRTGTPLVATNDCHYLNPGDDKL
                     QEILLCLQTGKTISDPTRMRFGSDQFYVKTAEEFERAFGHAAPDALANTLAIAERCNV
                     KIEVGVNKLPEIALPPGVTADGKLREIATEGLDRRLEERSKREGDLPQALVEEYRRRL
                     AYELSVIEKTGFASYFLIVADFIGWAKDAKIPVGPGRGSAAGSLVAFCVRITEVDSIR
                     YKLLFERFLNPERVSLPDIDCDFCKNRREEVIAYVQRKYGEENVAQLITFGTWKPRGA
                     VRDVGRVLEMPYAEVDRIAKMIPADLKMTVEDALKAEPRFRGMIDANPKIADLFRFAG
                     EIEGRNRHAGTHASAVVIANRPITDYCPLYRQATGEITTQYGMDPIARVGLVKFDFLG
                     LRTLTAIDDTLKLLKEIRGIEIDPNALELTDTETYEVLGRGDTVGVFQAESAGFTQLV
                     KNLKPDQFNHLIDMVALHRPGPLQSGMAADFVERRHGRRKVEFLLPQLKEILGDTYGV
                     IVYQEQVMEIAKALGGFTLGEADVLRKAMGKKDDALMDRQKAHFLEGAKGNGIPDAKA
                     AAIFDLMAQFGGYGFNKSHSAAYALLAYQTAYLKTHYPVEYFSALMTSESGDTDKIIR
                     YIAYCREKGIPILPPDVNESRYAFFPSDRAIRFGLSAIKGLGASAIDAILEARRESPF
                     RSVRDLLSRVDLRKVNKRAVESLIKSGALDSLDPDRGKVFGDLPALIEEAQAEVRRRE
                     SGQFALFGETSGGSPRKRGRKGEAAAPSWSRPERLTFEKETLGFYITGHPMDSFAGEI
                     ALYANTTTGKLSLLKHDAEVRIGGLVTGLKEKMTRRGDKMANWTLEDLEGTVDVVVFP
                     RQLPECREALGSPDPVFLVGRISIEEQGMKIHTEEVFRMENVRERLAKSVHFHLLLDR
                     MSPGDIAELRQTILRNAGEKKGFLHAIRPGEFDAVIALPDGCGVAPSLELARDLRGRF
                     GYDV"
BASE COUNT          746 a         1184 c         1280 g          615 t
ORIGIN      
        1 ccagccgttt cccgggccgg gtctcgcggt ccgcatcatc gggccggtca ccgaagagcg
       61 cctgcgcatc ctgcgcgagg cggacctgat cgtcgacgcg gagatccggg cggcggggct
      121 ctacgagtcg atctggcaat cgttcgccgt ccttctcccg atcaagacgg tcggcgtgat
      181 gggagatttc cgaacctatg agaacgtcgc cgccgtccgc gcggtccaca gccaggacgg
      241 gatgaccgcc gattgggtcc ggctcccgta cgacgtgctg cagcgaatct ccacgcggat
      301 catcaacggg gtgaagggga tcaaccgcgt cgcatacgac atctcctcga agcccccggg
      361 aaccatcgag tgggaatgaa gagcttcatc cacctccacc tccattcgga atacagcctc
      421 ctcgacggga cgatccagtt caagccgctg atcgagcgtg tgaagaagct gaagatgccc
      481 gctgtcgccg tcaccgatca cggcgggatg atggggacga tcggattcta cgaggaggcg
      541 ttgaagggcg gcgtgaaacc gatcctcggg acggagatct acgtcgcccc ggaatcccgc
      601 acggagcgga aagtcgctcc ctcgggcgaa tacgcctatc acctgatcct gctggcggaa
      661 aacgagatcg ggtacgggaa cctcctgcgg ctctcctccc tcgcccacac ggaagggttc
      721 tattacaagc cgagggtcga caaggaactg ctgcggagat actccgaagg gctgatcgcg
      781 acgtcggcat gcctgcaggg cgagatcccg ttcgcgatgg ggaccgaggg cgaggcgaag
      841 gcgctcgagg tgctggaaga gtacaagtcg atcttcaccg acgggcgctt ctacatcgag
      901 atccaggaca acggtcttcc ggaccaggcg aagatgaacc cgctcctcat caagcttgcg
      961 cgccggaccg gaaccccgct ggtcgcgacg aacgactgcc actacctcaa ccccggggac
     1021 gacaaactcc aggagatcct tctttgcctg cagacgggga agacgatctc ggatccgacc
     1081 cggatgcggt tcggctcgga ccagttctac gtgaagaccg ccgaagagtt cgagcgggcg
     1141 ttcgggcacg ccgccccgga cgctcttgcg aacacgctgg cgatcgcgga gcggtgcaac
     1201 gtgaagatcg aggtcggggt aaacaagctc cccgagatcg ccttgccccc gggggtcacc
     1261 gcggacggga agttgcggga gattgcgaca gagggtctcg accggcggct cgaggagcgg
     1321 tcgaaacggg agggggatct gccgcaggct ctcgtcgagg agtatcgtcg ccgcctcgcg
     1381 tacgagctct ccgtcatcga gaagaccggg ttcgcgagtt acttcctgat cgtcgccgac
     1441 ttcatcggtt gggcgaagga tgcaaagatc ccggtggggc caggccgcgg cagcgcggcg
     1501 ggaagcctgg ttgcgttctg cgtccggatc accgaggttg attcgatccg gtacaagctc
     1561 ctcttcgaac ggttcctgaa ccccgagcgg gtgagcctcc ccgacatcga ttgcgacttc
     1621 tgcaagaacc ggcgcgagga agtgatcgcc tacgtccaga ggaagtacgg cgaggagaac
     1681 gtcgcccagc tcatcacgtt cgggacgtgg aagccccggg gcgccgttcg cgacgtcgga
     1741 agggtcctcg agatgccgta cgccgaggtc gaccggatcg caaagatgat ccctgccgac
     1801 ctcaagatga cggtggaaga cgcgttgaag gcggaaccgc gtttccgggg aatgatcgac
     1861 gcgaacccga agatcgcaga cctctttcgc ttcgcggggg agatcgaggg gcgcaatcgg
     1921 cacgccggga cccacgcctc ggcggtggtg atcgccaacc ggccgatcac cgactattgt
     1981 cccctctacc ggcaggccac gggcgagatc acaacacagt acgggatgga cccgatcgca
     2041 cgggtggggc tcgtcaagtt cgacttcctc ggcctgcgca cgctgaccgc catcgacgac
     2101 acgctgaagc ttctaaagga aatccgcggg atcgagatcg acccgaacgc cctcgagctc
     2161 acggacacgg aaacgtacga ggtgctgggg cgcggcgaca cggtgggggt cttccaggcg
     2221 gaaagcgcgg ggttcaccca gctcgtcaag aacctcaagc ccgaccagtt caatcacctg
     2281 atcgacatgg tcgccctgca ccgccccggg cctctccaga gcggaatggc agccgatttc
     2341 gtggagcggc ggcacgggag gcggaaggtg gagttcctcc ttcctcaact gaaggagatc
     2401 ctcggggaca cgtatggcgt catcgtctac caggaacagg tgatggagat cgccaaggcg
     2461 ctgggcgggt tcacgcttgg cgaggctgac gttctccgga aggcgatggg gaaaaaggac
     2521 gacgccctga tggaccggca gaaggcccac ttcctggagg gagcgaaggg gaacggaatc
     2581 cccgatgcga aggcggccgc gatcttcgac ctgatggcgc agttcggcgg gtacggtttc
     2641 aacaagtccc acagcgccgc gtacgccctg ctggcctacc agaccgcgta cctcaagacg
     2701 cattaccccg tggaatattt cagcgccctg atgacctccg agtccgggga taccgacaag
     2761 atcatccggt acatcgcgta ttgccgggag aaggggatcc cgatcctccc gccggacgtg
     2821 aacgaatcgc gatacgcctt ttttccctcc gatcgggcga tccgcttcgg cctttccgcc
     2881 atcaagggac tcggggcttc ggcgatcgac gccattctcg aggcgcgcag ggaaagcccc
     2941 ttccgctccg tccgcgacct cctctcacgg gtcgacctgc gcaaggtgaa caagcgtgcg
     3001 gtggagagcc tgatcaagtc cggggccctc gactcactcg acccggatcg cggcaaggtg
     3061 ttcggggatc tcccggccct catcgaggag gcgcaggcgg aggtccgaag gcgcgagtcc
     3121 ggccagttcg cgcttttcgg ggaaacgtcc ggggggagcc cccggaagcg gggacggaaa
     3181 ggggaggccg cggcgccttc gtggtcccgc ccggagcggc tcaccttcga gaaggagacg
     3241 ttaggcttct acatcacagg gcacccgatg gactccttcg ccggggagat cgcgctgtac
     3301 gcgaacacaa ccaccggcaa gctctccttg ctgaagcacg acgccgaggt ccggatcggc
     3361 ggtctcgtca cagggctgaa ggaaaaaatg acccggcggg gtgacaagat ggccaactgg
     3421 acgctggagg atctcgaggg gacggtggac gtcgtcgtct tcccgaggca gctaccggag
     3481 tgccgcgagg cgttggggag cccggacccc gtctttctcg tcggcaggat cagcatcgag
     3541 gagcagggaa tgaaaatcca cacggaggag gttttccgga tggagaacgt ccgggaacgt
     3601 ctcgcgaaat cggtgcactt ccatctcctg ctggaccgga tgagccccgg ggacatcgcc
     3661 gaacttcggc agacgatcct tcgcaacgcc ggagagaaga aggggttcct ccacgcgatc
     3721 cgtccgggag agttcgatgc ggtgatcgcc ctgcccgacg gttgcggtgt ggcgccgtcg
     3781 ctggagctgg cgcgggacct tcgcggccgc ttcggctacg acgtg
//
LOCUS       DASVJL010000226         6362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_410023, whole genome shotgun sequence.
ACCESSION   DASVJL010000226 DASVJL010000000
VERSION     DASVJL010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410023"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VF357_04615"
     CDS             complement(<1..699)
                     /locus_tag="VF357_04615"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX7523462.1"
                     /translation="MASFVSRANWAEISPAAREALKIRILDSLGCAYGAIAGEPVRML
                     RKQVDEFGGRPLCTLIGGGHSAPDRAAFYNGALVRYLDYNDSYLAKGETCHPSDSLGA
                     VLAAAEYAGIPGKEFLTALAVAYQVQCRLSDVAPVRARGFDHVTHGAIAAAAGTAKAL
                     RLDPAKIANAVAIAGTANNALRVTRTGELSHWKGLAAPNAGAAGLQAAFLAMRGVTGP
                     REMFEGVKGWMQVVS"
     gene            complement(759..1664)
                     /locus_tag="VF357_04620"
     CDS             complement(759..1664)
                     /locus_tag="VF357_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX7523463.1"
                     /translation="MEHDTERSFGFLHINERGGKPRRTGVTEIRGPYYSPFGKRALLD
                     IFETMGAYVDVLKFAGGSFVLMPESAVRELIDTCHSHDVLVSTGGFIERVLTMGPEAV
                     DGYLEECKRLGFDIVEVSSGFISVPDDDLVRLVEKVRNLDMLPKPEVGIQFGAGGASS
                     VAELDAEGTSDPSRAIRQAKRYLEAGAHLIMIESEGITEQVKSWRTDVPARFINELGL
                     DKVMFEAADPEVFTWYVKSYGPEVNLFVDHSQIVQLECLRSGIWGTKSTWGRVVTYKG
                     SGKSVTAGMSPVRHIATRPKARGPV"
     gene            1971..2975
                     /locus_tag="VF357_04625"
     CDS             1971..2975
                     /locus_tag="VF357_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7523464.1"
                     /translation="MTKVRDRRRISRVTVEELLGEMHRSVDGLGEDGVSRADLKLLCR
                     ALKELRHAFRFFDGLRERPKVTVFGSARLPKGSPAYRQAESFGRAMARSGWFVVTGAG
                     EGIMEAAHIGAGREHSIGVNILLPFEQSENTVMERNPNLIHTKYFFTRKLLFLKESSG
                     VVLFPGGFGTMDEAFEVLTLLQTGKTHLFPVVLVDDPRGAYWATWRRFLVDNLLETGM
                     ISAEDFSLFRITSSVEEAVREIHGFYRVFHSMRYAGRDLVIRIREHLAPDTLASLNRD
                     FPDLLSEGSISQGKALPEEADEPEIADLPRIVFRFNSGRQGRLRQLIDQLNGLVDDDH
                     "
     gene            complement(3398..3472)
                     /locus_tag="VF357_04630"
     tRNA            complement(3398..3472)
                     /locus_tag="VF357_04630"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3438..3440),aa:Val,seq:cac)
     gene            complement(3550..4317)
                     /locus_tag="VF357_04635"
     CDS             complement(3550..4317)
                     /locus_tag="VF357_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523465.1"
                     /translation="MRRILQVVAVTAGVLLMAGSAFAASGFEVGARGAYWFPKLTGSA
                     QTNATGDTRFDFKDTLGVKDENIPYGEAFLRFGNTTLRVGYTQIKFDGNKELTETVVF
                     NGSTFSATDNVISKLDMKMIDGEVQYDFLRPDVGIAGFNIGLILKVKYVDGKVELRSA
                     SQGATLKDFKAPIPMIGAAAGVGFLKDMVRVDARAAGIAYSGNHLYDADAYASFAPLP
                     FFRIQGGYRYIDLKIDKDDTLASFKLKGPYLGAQLSF"
     gene            4401..4496
                     /locus_tag="VF357_04640"
     tRNA            4401..4496
                     /locus_tag="VF357_04640"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4436..4438,aa:Sec,seq:tca)
     gene            complement(4629..5231)
                     /locus_tag="VF357_04645"
     CDS             complement(4629..5231)
                     /locus_tag="VF357_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEX7523466.1"
                     /translation="MRLLCDIPFGTEALVHICCAPDASYGIRALRDRFDVTGFFYNPN
                     IDSGEEFRKRLLATLELREKDPFSLVVGAGGEEAWEDAVRGMEGEPERGRRCEACVRL
                     RLRETARKAAELAMPAFGTVLTVSPKKDAAMVNRIGREEGEHSGIRFVEADLKKGDGY
                     LKSVRASKELGLYRQSYCGCRYSYREPSEKTGGSVRESPR"
     gene            5424..6125
                     /locus_tag="VF357_04650"
     CDS             5424..6125
                     /locus_tag="VF357_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7523467.1"
                     /translation="MFNVPLLSILGVHEMGHYTAARRHKVEVTPPFFLPAPSFIGTFG
                     AFIRIRSAIPHRNALFDVGIAGPLAGAMVAIPVLMIGLGMSEVRAAGPGGLPLGESLI
                     FKAAAWVALGPVPKGYDVILHPVAFAGWIGLFVTMLNLIPAGQLDGGHIAYALFGDRY
                     AETARLVPYALLLMGILWVGWLVWAGLLFILGTRHPRTVVDEVPLSAGRKALGGIAVL
                     LFLLCFSLNPFPVGR"
     gene            6178..>6362
                     /locus_tag="VF357_04655"
     CDS             6178..>6362
                     /locus_tag="VF357_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523468.1"
                     /translation="MYALCVFGLLVPWMTREAVDAVSTARGGGPPGEGRLLRAVGLMV
                     LFSILYAFFRFLSRRSL"
BASE COUNT         1061 a         2101 c         1955 g         1245 t
ORIGIN      
        1 tgaaacgact tgcatccacc ccttgacgcc ctcgaacatc tcccgcgggc cggtgacgcc
       61 ccgcatggca aggaaggcgg cctgcaggcc ggcggccccg gcgttcggag cggcaagccc
      121 tttccagtgc gacagttctc ccgtgcgggt gacacgcaac gcgttgttcg ccgtgcctgc
      181 gatggccacg gcgttcgcga tctttgcggg atcgaggcgc agcgctttcg ccgtgcccgc
      241 ggcggcggcg atcgccccgt gggtcacatg gtcgaacccg cgcgcccgga ccggggccac
      301 gtcggagagg cggcactgga cctggtacgc caccgccagc gcggtgagga actccttccc
      361 cgggatcccg gcgtattccg ccgccgccag caccgccccg aggctgtcgc tggggtggca
      421 cgtttccccc ttcgctaggt agctgtcgtt gtagtccagg tatctgacca gcgctccgtt
      481 gtagaaggcg gcccggtccg gcgcggaatg cccgcctccg atcagtgtgc atagcggcct
      541 tcccccgaac tcgtccacct gtttccgcag catccgcacg ggttcccctg cgatcgcccc
      601 gtacgcgcag ccgagggaat cgaggatgcg aatcttcaac gcctcccggg cggccgggga
      661 gatctccgcc cagttcgccc tggacacgaa cgacgccaat ctctccgcct gcgtccgggg
      721 ttcgtcgaga tgccgggcgg ccgcgccacg gtacggtgtc atactgggcc cctggccttc
      781 gggcgggtcg cgatgtgccg cacgggggac atcccggcgg tcacggattt cccgctcccc
      841 ttgtaggtca ccacgcggcc ccacgtgctc ttcgtccccc agattcccga ccgcaggcat
      901 tcgagctgga cgatctggga gtggtccacg aagaggttga cctccggacc gtacgacttc
      961 acgtaccagg tgaacacctc cgggtcggcc gcctcaaaca tcaccttgtc cagcccgagt
     1021 tcgttgatga accgggccgg cacgtccgtg cgccacgact tgacctgctc cgtgatcccc
     1081 tccgattcga tcatgatgag atgcgccccc gcctccaggt accgtttcgc ctggcggatc
     1141 gcgcgggacg ggtcggaggt cccctccgcg tccagttccg ccaccgagct ggctcctccg
     1201 gcgccgaact gtatgccgac ctcgggcttc gggagcatgt ccaggttccg taccttctcc
     1261 acgaggcgga cgaggtcgtc gtcggggacc gagatgaacc cgctggacac ttccacgatg
     1321 tcgaacccca gtcgtttgca ctcctcgagg tacccgtcca cggcttcggg ccccatcgtc
     1381 aggacgcgct cgatgaaccc tccggtggac acaaggacgt cgtgcgaatg acaggtgtcg
     1441 atcagctcgc gcacggcgct ctccggcatc aggacgaagg aacccccggc gaatttgagc
     1501 acgtcgacgt aggcccccat cgtttcaaag atgtccagta gtgcccgctt tccaaagggg
     1561 gagtagtacg gcccccggat ctccgtgaca ccggtccggc gcggcttgcc gccccgctca
     1621 tttatgtgaa gaaaaccgaa actcctttcg gtatcgtgct ccatggaaat ccctccttcc
     1681 caacccggtt tcttctcgtc cctctctata agatggaacc ggcacaacgg gtcgtttcgc
     1741 cagggacaga cttcccggtg cgatgccgga gattgcagag tcgacggaag gggcgttggt
     1801 gcggttcccg aagcagaccc gtgaggcgga accctccaag taggcaaccg gagacgtttt
     1861 cggttcatca aaagccgttg ggtggaaaat cctgtaaaag ggggttgccc ccaccttttc
     1921 tttcgcagca gtgcttccgg ccagcgaacc gggaagaagc ggagaggcgc atgacgaagg
     1981 tccgcgacag gcgaagaata tcccgggtga ccgtagagga actcctcggc gagatgcaca
     2041 ggagcgtcga cgggttgggg gaagacggag taagccgggc ggatctgaaa ctcctctgcc
     2101 gcgccctgaa ggaacttcgc cacgccttcc ggttcttcga cgggctccgg gaacgcccaa
     2161 aggtgacggt gttcgggtcg gcgcgcctgc caaagggctc gcccgcgtac cggcaggccg
     2221 agtcgttcgg cagggcgatg gcgcggtcgg ggtggttcgt cgtcaccggc gccggcgagg
     2281 ggatcatgga ggcggcccac atcggagccg gtcgggagca ctcgatcggt gtgaacatcc
     2341 tgctcccgtt cgagcagtcc gaaaacacgg tcatggagag gaacccgaat ctcatccata
     2401 cgaaatattt cttcaccagg aagctgctct ttctcaagga gtcgagcggc gtcgtcctct
     2461 ttccgggagg attcgggacg atggacgagg cgttcgaggt gttgaccctc ctccaaacgg
     2521 ggaagaccca ccttttcccc gtcgtgctcg ttgacgatcc ccgtggggcc tattgggcga
     2581 cgtggcgccg gttcctcgtg gacaacttgc tggaaacggg aatgatctcc gccgaagatt
     2641 tctccctgtt ccggatcacc tcgagcgtgg aagaagccgt ccgggagatc cacggctttt
     2701 atcgggtgtt ccacagcatg cggtacgcgg gccgggacct ggtgattcgt atccgggagc
     2761 atctcgcccc cgacacgctc gcctccctga acagagattt cccggatctc ctctcggagg
     2821 gatcgatctc ccagggaaaa gcccttcccg aggaggcgga cgagccggag atcgccgatc
     2881 tgccacggat cgttttccgg ttcaacagcg gcagacaagg ccggcttcgc cagttgatcg
     2941 accaactcaa tggactggtc gacgatgatc actgaccgaa accgatgaaa ttgtcgaggg
     3001 tcgatgtgga aatgggtagt gactggggaa acccgcgaag ttagggaggc ctaagagggg
     3061 acaaggcggc tgaccttcgc cgccatgatg aagtcgtttt cgtgcaagcc ccgaatcgag
     3121 tgggtctgga accgtaccgt gcagtaaccc cagccgacgg acagggatcc cttttccctt
     3181 gttcaggaag gcacccttgt cctatcgcct gtcttgtgga gactcgaact ctccagtagc
     3241 aatgatttat aaatcgggag gcatcggagg gagtggcgat ggtataccgc gaacgctccc
     3301 gccggtggcg gggaatcttg tgtcaaggtc gtgtgacaag tagcaaatgt gcaagaaaaa
     3361 gggctgacgg ggaaacccgc cagcccttgt ctttccttgg tgggcgctac aggacttgaa
     3421 cctgtgactt ccaccgtgtg aaggtggcac tctaccgctg agttaagcgc cccgagagac
     3481 tcctattgta ccttcctgtc ttcccggttc aacggttttt tctcgcggtc ttcccccggg
     3541 ggctgcggtt cagaacgaga gctgcgctcc gaggtacggt cctttcagct tgaaagaggc
     3601 cagcgtgtcg tccttgtcga tcttcaggtc gatgtaccgg tatcctccct ggatccggaa
     3661 gaacggcagg ggggcgaacg aggcgtacgc gtccgcgtcg tacaggtggt tcccggagta
     3721 ggcgatccct gccgcgcggg cgtcgacccg caccatgtcc ttgaggaatc cgacccccgc
     3781 cgccgcgccg atcatcggga tcggcgcctt gaagtccttg agggtcgccc cctgggatgc
     3841 gctgcgcagc tccaccttcc cgtccacgta tttcaccttc aggatcagcc cgatgttgaa
     3901 accggcgata ccgacgtcgg gacggaggaa gtcgtactgc acctccccgt cgatcatctt
     3961 catgtcgagc ttggagatca cgttgtccgt cgcggaaaag gtcgacccgt tgaatacgac
     4021 ggtctcggtc aactccttgt tcccgtcgaa cttgatctgg gtgtacccca cccggagcgt
     4081 cgtgttcccg aaccgcagga acgcctctcc gtacgggata ttctcgtcct tcactcccaa
     4141 ggtgtccttg aaatcgaatc gggtgtcgcc ggtcgcgttt gtctgcgcgc tgccggtcag
     4201 tttcgggaac cagtacgccc cgcgggctcc cacctcgaac cccgaagccg caaacgcgct
     4261 tcccgccatg agcaggacac ccgccgtcac cgccacaacc tggagaatcc gtcgcatcac
     4321 actctccttt ctccatccgt gaaaatcggt acttggtgta ttatcggcag gaagggcacg
     4381 gaaccttacg cccaatggac ggaagcggat ggggcgctgg tggccccccc ggacttcaaa
     4441 tccggtcggg aggcggaaac gtctccggtg ggttcgattc cctcacgctt ccgccatgct
     4501 tcctcccaat ggatgcccgg ggaatcgaag cgagaagccc gccgacgagc aggaggaaaa
     4561 gcgatccggt ccgtagcgcg cgtcagtgcg acaagccggc cctcaaatag cgagcgggcg
     4621 aacgcgagct aacgtgggga ttccctcaca cttccgccag tcttttccga gggttccctg
     4681 taggagtacc tgcacccgca gtaactctgg cgatatagcc ccagttcctt gctggcgcgg
     4741 acgcttttca gatacccgtc cccctttttg aggtccgcct cgacgaaccg gatcccggag
     4801 tgctccccct cttcgcgtcc aatgcggttt accatcgcgg cgtccttctt cgggctgacg
     4861 gtcagcaccg tgccgaaggc gggcatcgcg agctccgccg ctttcctcgc cgtttcccgc
     4921 agccgcagcc ggacgcacgc ctcgcaacgg cgtccccgct ctggctcgcc ctccatcccc
     4981 cggacggcat cttcccacgc ctcctcaccg ccggcgccga ccaccaggga gaaggggtct
     5041 ttctcccgca gctcgagggt cgcgaggagc cgtttccgga attcttcccc ggaatcgatg
     5101 tttgggttat aaaagaagcc ggtgacgtcg aaccggtccc gcaacgcgcg aatgccgtaa
     5161 gaggcgtcgg gggcgcagca gatgtggacc agcgcttccg tgccgaacgg gatgtcgcaa
     5221 aggagtcgca cgagggaacc cgcccccctt gtcggtgatc ttacagcgga tagtacaccg
     5281 aatttttccc cgaaggggat ggagagattc caacggtacc gatcctgctg cttctggcca
     5341 ccttcgtcac gacactgttc gcggggtcgt tcctcgccgg ggggaccccg ttcgttcgcc
     5401 ccgcggatct ccttctcggc ctcatgttca acgtcccgtt gctgtcgatc ctcggggtcc
     5461 acgagatggg gcactacacg gcggcgcggc ggcacaaggt cgaggtgacc ccccccttct
     5521 tccttcccgc gccatcgttc atcggcacgt tcggcgcctt catacggatc cggtcggcca
     5581 taccgcaccg caacgccctt ttcgacgtgg ggatcgcggg gcctttggcc ggggccatgg
     5641 tggcaattcc cgtcctgatg atcggcttgg gcatgtccga agtgcgtgcg gccgggcctg
     5701 gagggctccc cctcggagag tcgctgatct tcaaggccgc cgcatgggtc gcgcttggcc
     5761 cggtcccgaa ggggtatgac gtgatccttc atcccgtcgc ctttgccggc tggatcggtc
     5821 tcttcgtcac gatgcttaac ctcattcccg ccgggcagct cgacggtggg cacatcgcct
     5881 acgccctctt tggggaccga tacgcggaaa cggcgcgcct cgtcccgtac gccctccttc
     5941 ttatggggat cctgtgggtc gggtggctgg tgtgggcagg ccttcttttt atcctcggga
     6001 cgcgacaccc gcggaccgtg gtcgacgagg tgccgctttc ggcgggacgg aaagctctcg
     6061 gcgggatcgc tgtcctcctg ttcctcctct gtttctccct taatccgttc ccggtcggca
     6121 ggtaagacca ttcgcgagct gctcaccctg cgcccgcact ttgccgcaca ccggcgggtg
     6181 tacgccctct gcgtcttcgg cctcctcgtc ccctggatga cccgggaggc agtcgacgcc
     6241 gtctccaccg cgcggggagg cggaccgccc ggcgagggcc gcctgctccg cgccgtcggg
     6301 ctcatggtcc tcttctccat cctgtacgcc ttcttccggt tcctttcgcg ccggtccctg
     6361 tt
//
LOCUS       DASVJL010000227         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_34402, whole genome shotgun sequence.
ACCESSION   DASVJL010000227 DASVJL010000000
VERSION     DASVJL010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34402"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1623)
                     /locus_tag="VF357_04660"
     CDS             complement(<1..>1623)
                     /locus_tag="VF357_04660"
                     /inference="COORDINATES: protein motif:HMM:NF021504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HEX7523469.1"
                     /translation="VDTIFEIGGQDSKFIGLENGVVTDFAMNDACAAGTGSFLEEQAE
                     RLGIRIKGEFAEMALSSSAPVRMGERCTVFMEQDLNNYLYRGARKNDLVAGLAYSVVM
                     NYLNRVVRGRKIGETIYFQGGTAYNDSVAAAFSQVLGRKIIVPPHNGVIGAIGMALLA
                     RDKVRATKEATTFRGFDLSKVDYRRREFVCKGCSNYCDMQEILIDDSRTYWGDKCSEK
                     YRKPARTGTKPVIDDLVARRIEWMDRLIEETPSSGPRGTVGFPRAMYFHERFPFWKGV
                     LTRMGFGVKVSPRTDKAIAREGFERTVAEPCYPIQVAHGHVGALLVSGVDFLFLPNVI
                     NLETTHTHTASQFCPWGQTLPFVVAAVPKWEKELRQKLLSPTVRFRDEEKYIVEDLLD
                     CFGPLGVSRKEIREGIRDGYREQAKLGQFLEVAGAEAIERVEKAGRDAIVLLGRPYNL
                     YDRDVNLNIPAKLRDQYGGNVIPIDFLPVDDIDIRDVNDNMFWNYGRKIIAAAKWCRN
                     RPKFHMIYITNFKCGPDSFVRHFITRASGSPYL"
BASE COUNT          269 a          552 c          499 g          303 t
ORIGIN      
        1 aggtacgggg acccggacgc cctcgtgatg aagtgtcgca cgaaggagtc ggggccgcac
       61 ttgaaattcg tgatgtagat catgtggaat ttcggccggt tccggcacca tttcgccgcg
      121 gcgatgatct tgcgcccgta attccagaac atgttgtcgt tcacgtcgcg gatgtcgatg
      181 tcgtccaccg ggaggaagtc gatcgggatg acgttgccgc cgtactggtc ccgaagcttc
      241 gccgggatgt tcaggttgac gtcccggtcg tacagattgt acggacgccc cagcaggacg
      301 atcgcgtccc tccccgcctt ctcgacccgc tcgatcgcct ccgcccccgc gacctcgagg
      361 aactggccga gcttggcctg ctcgcggtac ccgtcgcgga tcccctcccg gatctccttc
      421 cgggatacgc ccagcggccc gaaacagtcg agcaggtcct caacgatgta cttctcttcg
      481 tcgcggaacc ggacggtggg cgagagcagc ttctgccgca gctctttctc ccatttgggg
      541 acggcggcaa cgacgaacgg cagcgtctgc ccccagggac agaattgcga tgccgtgtgg
      601 gtgtgggtcg tctccaagtt gatcacgttc ggcaggaaga ggaagtcgac accggagacc
      661 agcagcgctc ccacgtgccc gtgggccacc tggatcgggt agcacggctc ggcgacggtc
      721 cgctcgaacc cctcgcgggc gatcgcctta tcggtgcgcg gcgacacctt gacaccgaac
      781 cccatcctcg taaggacccc cttccagaac gggaaccgct cgtggaaata catcgcccgt
      841 gggaacccga cggtcccgcg cgggccgctc gaaggagtct cctcgatgag ccggtccatc
      901 cattcgatcc tgcgcgccac caggtcgtcg atcaccggct tcgtgccggt gcgggccggt
      961 ttgcggtact tctcggagca cttgtcgccc cagtaggtgc ggctgtcgtc gatcaggatc
     1021 tcctgcatgt cgcagtagtt gctgcacccc ttgcagacga actcccgccg ccggtagtcg
     1081 accttcgaga gatcgaagcc gcggaacgtc gtggcctcct tcgtcgcccg gaccttgtcc
     1141 cgggcgagca gcgccatccc gatcgccccg atcaccccgt tgtgcggcgg aacgatgatc
     1201 ttgcggccca gcacctggga gaatgcggcg gcgaccgaat cgttgtacgc cgtccccccc
     1261 tggaagtaga tcgtctcgcc gatcttccgg ccgcgcacca cgcggttcag gtaattcatg
     1321 acgaccgagt acgcaagacc cgcaacgagg tcgttcttcc gggcgccccg gtagagatag
     1381 ttgttcaggt cctgctccat gaacacggtg caccgctccc ccatgcggac cggggcgctc
     1441 gaggagaggg ccatctcggc gaactctccc ttgatccgga ttccgagccg ctccgcctgc
     1501 tcctcgagga acgacccggt cccggcggcg cacgcgtcgt tcatcgcgaa atcggtgacc
     1561 acgccgtttt cgaggccgat gaacttggag tcctgcccgc cgatctcgaa gatcgtgtcg
     1621 acc
//
LOCUS       DASVJL010000228         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1442360, whole genome shotgun sequence.
ACCESSION   DASVJL010000228 DASVJL010000000
VERSION     DASVJL010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442360"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1962)
                     /locus_tag="VF357_04665"
     CDS             complement(181..1962)
                     /locus_tag="VF357_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7523470.1"
                     /translation="MTAGRVDRGGEFLMTGAAGKRIFTPRDFPPDRRQIAWTAEEFVR
                     KEVLPDLDRMERRDFALVVAKMRRCAELGLFLADVPEEYGGLDLDKATSMLIAEKMGP
                     SGSFSITYSGQTGIGSLPLVYYGTQAQKERYLGLLTSGEWIGAYCLTEPDCGSDPLSA
                     RTTARLSEDGRHFLLNGVKQFITNAAFADLFTVFAKIDGKRFSAFLVEKRFPGVSVGP
                     EEEKMGLKGSSTAQVILEDAKVPVENLLGEAGKGHKIAFNVLNIGRLKLAATVTGMAK
                     GAFAEAAVYAGQRRQFGRVIGEFGAIREKLADMAAAIFSCESVVYRIAGLLDDGIAAL
                     DRSRADYMDLYRKAIEEYAGECAIAKVHCTEALAYVADEAVQVHGGYGYIREYPVERY
                     YRDERINRIFEGTNEINRILIPTVLFRRGEGGEFDLREKVAEAGARAREKKKERPAGN
                     GPFGEELLFGDGLKQTFLAVLGASWEARESQEVLLALADMIIEIFAMETVALRAEKDY
                     AAAGAREKDLLGAVVKVALFSGAGRFQTAASRCGAYALSGGALASCLELVSRRSVFPV
                     GGLLEAKRLLAEASLEDGRYVFRSHET"
BASE COUNT          379 a          783 c          633 g          395 t
ORIGIN      
        1 tcgggggact ccccgaccca acggcggaac atcaccccca ggctctcctc gtacggctcg
       61 agcgccttcg gggaacggag gagataccct ccctccgggg tcttctgcac ctcgatttca
      121 gcgggcgcca gatccagcgg cccgaaatcc gttcctccca ccggtacctt ccccttttcc
      181 tcaggtttca tgggaccgga acacgtatct gccatcctcc agagacgcct ccgcaaggag
      241 gcgcttcgcc tccagcagcc cgccgaccgg gaatacggaa cggcgggaaa ccaactccag
      301 gcacgaagcg agcgcgccgc ccgaaagcgc gtaggcgccg cagcgggacg cagccgtctg
      361 gaaccggccc gcgccggaaa agagcgcgac cttgacgacc gcgcccagga gatccttctc
      421 cctcgcaccc gcggcggcgt aatccttttc ggcccggagg gcgaccgtct ccatggcgaa
      481 gatctcgatg atcatgtcgg ccagcgcgag cagcacttcc tggctttccc tcgcctccca
      541 cgacgcaccc aggaccgcca ggaacgtctg cttcaggccg tcgccgaaga gaagttcctc
      601 cccgaaaggg ccattccctg ccggcctctc cttttttttc tccctcgcgc gggcgccggc
      661 ttcggccacc ttttcacgaa ggtcgaactc tcccccttcg cccctccgga acagcacggt
      721 gggtatgaga atgcggttga tctcgttggt cccctcgaag atccggttga tccgttcgtc
      781 cctgtagtac cgctcgaccg ggtactcgcg gatgtacccg tagccgccgt gcacctggac
      841 cgcctcgtcg gccacgtacg ccagcgcttc cgtgcagtgg accttggcga tggcgcactc
      901 ccctgcgtac tcctcgatcg ctttccggta caggtccatg taatcggccc gggagcggtc
      961 cagcgccgcg atcccgtcgt ccagcagccc ggcaatgcgg tagacgaccg actcgcacga
     1021 gaagatcgcc gccgccatgt ccgccagctt ctcccggatg gcgccgaatt caccgatgac
     1081 ccggccgaac tgcctccgct gccctgcgta cacggcagcc tcggcgaacg cgcctttggc
     1141 cattccggtc accgtggcgg caagcttcag ccgcccgatg ttcaggacgt tgaatgcgat
     1201 cttgtgcccc ttcccggcct cccccagcag gttttcgacc ggcacctttg cgtcttccag
     1261 gatgacctgc gccgtggagg agcctttcag ccccatcttc tcttcctccg ggccgacgga
     1321 tacccccggg aaccgcttct ccacgaggaa cgcggagaac cgctttccat cgatcttcgc
     1381 gaacaccgtg aacagatcgg cgaatgcggc gttggtgatg aactgcttca ccccgttcag
     1441 aaggaagtgg cggccgtcct ccgataatct cgccgtggtc ctggcgctca gcgggtcgct
     1501 cccgcagtcg ggctcggtga ggcagtatgc cccgatccat tccccggagg taagcaagcc
     1561 gagataccgt tctttctggg cctgcgtccc gtagtagacg agcggcagcg acccgatgcc
     1621 ggtctgtccg ctgtaggtga tggagaacga accggacggc cccatcttct cggcgatcag
     1681 catgctggta gccttgtcaa gatcgaggcc tccgtactcc tcgggaacgt ccgcgaggaa
     1741 cagcccgagt tcggcgcacc ggcgcatctt cgcgacaacc aacgcgaagt cccgccgctc
     1801 catccggtcg aggtccggga ggacctcctt ccgcacgaac tcttcggcgg tccacgcgat
     1861 ctgccgccgg tccggcggga aatcacgggg ggtgaagatc ctcttcccgg cagcgccggt
     1921 catcaggaac tccccgcccc tgtcaaccct ccccgccgtc accaccagcc ccctattgtg
     1981 gaattggaca ttgcgtgcgg tattgtggta ttaaggtacc atatggcaat gaaagaacca
     2041 atcctcatac ggaacggcgt gcccacggac ctcgacgcca tcatcgagat cgacgacgtc
     2101 ggtcccaagg aggagaaacc ggcttactgg cgcgggatct tcgcgcatta cgggagcggc
     2161 gagaagaagg accggatctt cctggtcgcg
//
LOCUS       DASVJL010000229         1932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_879853, whole genome shotgun sequence.
ACCESSION   DASVJL010000229 DASVJL010000000
VERSION     DASVJL010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879853"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VF357_04670"
     CDS             <1..472
                     /locus_tag="VF357_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX7523471.1"
                     /translation="RGPVEKALSDLGEMLRSEGVTALFFPEDDAAAREIAEALVPFGP
                     FNLTTGAEVRGGSTAVTAGFRAAEAAIAETGTIVETSAGVKSLLPGLIADVHVSIVPA
                     AVVVARMEEALAAFTADPPRNISFLSGPSKTADIEQTLTIGAHGPKKAIALLLT"
     gene            complement(537..1499)
                     /locus_tag="VF357_04675"
     CDS             complement(537..1499)
                     /locus_tag="VF357_04675"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX7523472.1"
                     /translation="MFLRGAHLDILQSVFPVFLVVATGVVLRRYRFLDGGFIDAANSL
                     VYYILLPALLFHEIGGTDFRQAFSGPLVVGGYAATLTTFLLAFLVSRALGLGPSETGA
                     FVQGSFRANLAYVGLPIVFNAVGPAGLGKAGIFLGLVVPLLNGLAIVALMAPHGAGKE
                     EGNATTASHIGREILTNPIILACLAGVAWSVLKLPLPGMIDRTLRLLTPATLPLSLLC
                     LGGSFSFERARKGFAVAALATFLKVGVLTGIGIALYRWMGLSGEDMRIGAIMLGCPTA
                     VVTYVMAARLRGDTDLAGTIVIASTAASAVTITGWLFLLRAFGW"
     gene            complement(1582..>1932)
                     /locus_tag="VF357_04680"
     CDS             complement(1582..>1932)
                     /locus_tag="VF357_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7523473.1"
                     /translation="RNFNMRVRSVVLIVLSVMLLAFAIGCSKQEPPPPAAPAAPAAVA
                     PAASAAPAADGKSLFEAKCSVCHGLDRPTARKETKEKWASIVKEMQGKKADWISDAEA
                     SNIVDYLTAQYGKK"
BASE COUNT          345 a          651 c          670 g          266 t
ORIGIN      
        1 ccgcgggccg gtggaaaagg cgttgtccga cctgggggaa atgctgcgct cggagggagt
       61 gaccgccctc ttcttcccgg aagacgacgc ggcggcgcgg gagatcgcgg aggcgctcgt
      121 cccgttcggg ccgttcaacc ttacgacggg ggcggaggtc cgcgggggga gcacggcggt
      181 caccgccggt ttccgcgcgg cggaggccgc gatcgccgaa acggggacga tcgtcgagac
      241 cagcgcgggg gtgaaatcgc tcctcccggg cctgatcgcg gacgtccacg tgtcgatcgt
      301 gcccgcagcc gtggtggtcg cccggatgga ggaggcgctg gcggctttca ccgccgaccc
      361 gccccggaac atctcgttcc tctccggccc gagcaaaacc gccgacatcg agcagactct
      421 taccatcggc gcccacgggc cgaagaaggc gatcgcactg ctgctgacgt aagacaaccc
      481 ctcctgcgga gtgacgtagc ggggtggaag cccccgcgag gaacccgcgg gaggggttac
      541 cacccgaacg cgcggagtag gaatagccac ccggtgattg tgacggcgga ggcggcggtc
      601 gaggcgatga cgatcgtccc ggcgaggtcc gtgtcgcccc gcaggcgcgc cgccatcacg
      661 taggtgacga ccgcggtggg gcacccgagc atgatcgccc cgatccgcat atcctccccc
      721 gacaacccca tccaccggta aagggcgatc ccgatccccg tcaggacgcc caccttcagg
      781 aatgtcgcga gcgccgcgac cgcgaatcct ttccgtgcgc gctcgaacga gaaggatccc
      841 ccgaggcaca gcagcgacag cggcaaggtg gccggcgtga gcaaccggag ggtccggtcg
      901 atcatcccgg gcaggggaag tttcagcacg ctccaggcga ccccggccag gcacgcgagg
      961 atgatggggt tggtcaagat ctcccgcccg atgtgcgacg ccgtggtcgc attcccctcc
     1021 tctttccccg cgccgtgagg ggccatcagc gccacgatcg ccagaccgtt cagcagcgga
     1081 acgaccagtc cgaggaagat ccccgccttc cccagccccg cgggccccac cgcattgaag
     1141 acgatcggca gccctacgta ggcgaggttg gcgcggaacg acccctggac gaacgccccc
     1201 gtttcggagg ggccgagccc aagggcgcgg gaaacgagga aggcgagcag gaaggtcgta
     1261 agcgtggcgg cgtacccccc gaccacgagg gggccgctga acgcctgccg gaaatcggtg
     1321 ccgccgatct cgtggaagag gagcgccggg agcaggatgt agtagacgag ggagttcgcc
     1381 gcgtcgatga acccgccgtc gaggaaccgg tagcggcgca ggacgacccc cgtcgccacc
     1441 acgaggaaca ccgggaagac cgactgcagg atgtccagat gcgccccccg aagaaaaatc
     1501 gggcggtccc ctgcgcctga gaccgcccgt tcgcgctacg ccgggaaggg tgttcgagcc
     1561 gcccgtcccc tgtcgatcgg attacttctt gccgtactgc gcggtgaggt agtcgacgat
     1621 gttcgacgct tcggcgtcgg agatccagtc cgccttcttc ccctgcatct ccttgacgat
     1681 gctcgcccac ttctccttcg tctcctttcg ggccgtcggc cggtcaagac cgtggcagac
     1741 gctgcacttc gcttcgaaga gggacttgcc gtctgcggca ggagccgcgg aagccgccgg
     1801 ggccaccgcc gcgggagccg cgggagccgc aggaggggga ggttcctgct tggagcagcc
     1861 gatggcgaag gcgagcagca tcacggataa gacaatcagc accacggagc gaacgcgcat
     1921 gttgaaattc ct
//
LOCUS       DASVJL010000230         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1442515, whole genome shotgun sequence.
ACCESSION   DASVJL010000230 DASVJL010000000
VERSION     DASVJL010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442515"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..>1636)
                     /gene="metG"
                     /locus_tag="VF357_04685"
     CDS             complement(171..>1636)
                     /gene="metG"
                     /locus_tag="VF357_04685"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX7523474.1"
                     /translation="TTIACDALARWHRMLGKRVFFLTGTDEHGEKVQKTAAAKGLSPR
                     ELADQVVTNFQGLTPALTISNTGFIRTTEPRHYASVQDLFRKSLANGDIYLGDYEGWY
                     CVPDEAYWTALQLVDGNCPACGRFVEMRKEPSYFFRLSKYQEPLLDYYRKHPAFIRPE
                     SRRNEVVAFVEGGLNDLSVSRTSLSWGIPVPEAPGHVIYVWYDALTNYITGLGYPSPT
                     ADFGTFWPADIHMVGKDILRFHAVYWPAFLMSAGIAPPLGVFAHGWWTVEGQKMSKSL
                     GNVVDPYEMVRKYGADAFRYFLLREVSFGLDGDFSEKELIKRANSELADKLGNLLNRS
                     LGMLGKYFAGVVPEPGPAEPEDAVLSACGRNSLPAVAAAMEEVAFHKALGAIIELVTK
                     GNEYVQSTQPWALAKDPGKRVRLGTVLYNALEASRVAALLMAPFTPTAAQKLWEALVP
                     GGKSLENVRIDRDGAWGGLAAGATLPNTCIVFPKIEV"
BASE COUNT          260 a          555 c          544 g          277 t
ORIGIN      
        1 atacggcccg ctcgccgctc tccgggtcga tgccgaccgt ggcgatccgg gtgacccccg
       61 cgtcgcgcgc ccggcgcacc acctcctcct ccgcgtcgta gagcggcgaa aggtcgaggt
      121 gcgcgtgagt gtcgaagaac atctggttgg gccgagggcc tacggacgac tcaaacttcg
      181 atcttcggga aaacgatgca cgtgttcggg agggtggcgc ccgccgcgag ccctccccac
      241 gcgccgtccc ggtcgatccg tacgttttcc agggatttgc ccccgggaac gagggcttcc
      301 cacagcttct gcgccgcggt cggggtgaac ggggccatca gcagcgcggc cacccgcgac
      361 gcctcgaggg cgttgtagag aaccgtcccc agccggacgc gctttcccgg gtccttcgcc
      421 agcgcccacg gctgcgtcga ctgcacgtat tcgttcccct tcgtcacgag ttcgatgatc
      481 gccccgagcg ccttgtggaa cgccacctcc tccatcgccg cagccacggc cggcaagctg
      541 ttgcgcccgc aggcggacag gaccgcgtcc tcgggttcgg cgggccccgg ctcggggacg
      601 acgccggcga aatatttccc gagcatcccg agcgatcggt tgagcaggtt gcccagcttg
      661 tcggcgagct ccgaatttgc gcgcttgatc agctccttct ccgagaagtc gccgtccagt
      721 ccgaaggaga cttcgcgcag caggaagtaa cggaacgcgt ccgccccgta tttccggacc
      781 atctcgtacg ggtcgacgac gttccccagc gatttgctca tcttttgccc ctcgacggtc
      841 caccatccgt gggcgaacac cccgagcggc ggcgcgatcc cggcggacat gaggaacgcg
      901 ggccagtaga cggcgtggaa ccggaggatg tctttcccca ccatgtggat gtcggcgggc
      961 cagaacgtcc cgaaatccgc cgtcggggaa gggtacccca gcccggtgat gtaattggtg
     1021 agggcgtcgt accagacgta aatcacgtgt cccggcgcct cgggcaccgg gatcccccag
     1081 gagagcgacg tgcgggagac ggacaggtcg ttcaggcccc cctcgacgaa ggcgaccacc
     1141 tcgttgcggc ggctctccgg ccggatgaag gccgggtgtt tccggtagta gtcgaggagc
     1201 ggctcctggt acttcgacag gcggaaaaag taggaaggct ccttccgcat ctccacgaag
     1261 cggccgcacg ccgggcaatt cccgtccaca agctgcagcg ccgtccagta cgcctcgtcg
     1321 gggacgcagt accacccctc gtagtcgccc aggtagatgt ccccgttcgc gagcgacttg
     1381 cgaaagaggt cctgcacgga ggcgtagtgc cgcggctccg tggtgcggat gaagccagtg
     1441 ttgctgatcg tcagggccgg ggtgaggccc tggaagttgg tcacgacctg gtcggcgagt
     1501 tcgcgggggg agaggccttt cgccgccgcg gtcttctgca ccttctcccc gtgctcgtcc
     1561 gttcccgtga ggaagaagac ccgcttcccc agcatccggt gccagcgcgc gagggcgtcg
     1621 caggcgatcg tcgtgt
//
LOCUS       DASVJL010000231         3962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_316636, whole genome shotgun sequence.
ACCESSION   DASVJL010000231 DASVJL010000000
VERSION     DASVJL010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316636"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VF357_04690"
     CDS             complement(<1..607)
                     /locus_tag="VF357_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523475.1"
                     /translation="MLKVENLSAGYGEVHVLERVSFQVRREEMTCILGPNGAGKTTLL
                     RSIIGLLPPTAGRIVFLEEEIQGKRTHAVVRKGMTMIPEGRRLWPPLTVRENLEMGAY
                     GEKEGEIVRERLERVFALFPHLRSRKGQFCGTLSGGEQQMVAIGRGLMGAPRLLLMDE
                     PSLGLAPLVVREMFEAIRKVVLAGTTVLLVEQNTQVALSASR"
     gene            complement(600..1388)
                     /locus_tag="VF357_04695"
     CDS             complement(600..1388)
                     /locus_tag="VF357_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523476.1"
                     /translation="MTEPASAGTAASLVGPRGKILLDVSNLSKDFGGLRAVSALSFRA
                     REGEFVGVIGPNGAGKTTLFALLSGFLPATGGEIRFRGVLTGALRTDQLCHLGMARTF
                     QVVRPFPEMTVFENVKVAAVFGKKERPIAAEIRREAEEALACTGLSSVAALPARSLNL
                     PQRKRLEMARALATRPKVLLLDEVLAGLNPREVEDAIPFVRSLNAERGITVLMIEHNL
                     RAIMGLSHRILVLNFGQLIFDGAPEDAVKDPGVVQAYLGERHDA"
     gene            complement(1385..2494)
                     /locus_tag="VF357_04700"
     CDS             complement(1385..2494)
                     /locus_tag="VF357_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7523477.1"
                     /translation="MFRDTLRKFVDKELEPISEKVEEEDRIPDETVAKMAELGLFGLA
                     IPADYGGFGMSVLGQCVAMEELSRTNACFRTRIGTNNGIGSMGIVIAGTEAQRRKFLP
                     KLATGEMIGAFALTEPNAGSDASAIRTTAVRKGDKFILNGMKHFITNGDIADVSTVMA
                     ITDKGKGTKGISAFLVEKGFKGFSVGTIERKMGFRGSHTCELVFDDCEVPAENLLGRE
                     GEGFKIAMKILDKGRLTMGACALGSLEKLIDLSRDYAAMRVTFGEPIINRQAIQWMLV
                     NMAIDAYAVRNAVYNAAWKMDRGDRVTKEAAMVKVLATEAAWRASDSAVQIHGGMGYM
                     KGMAVERFYRDLRLLRIYEGTSEILRMVISREMTR"
     gene            complement(2851..>3962)
                     /locus_tag="VF357_04705"
     CDS             complement(2851..>3962)
                     /locus_tag="VF357_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7523478.1"
                     /translation="HRYEKAMTALLMLALAVPAFGQSPEEDARRYMVRGMAAVEMAKT
                     EDDLSNAAVEFKKAIEIAPNMAAAWYNLASVQSKIGQLKEAIESYQKYLALTPKAEDT
                     QRVKDEITKLEYRMEQTGRLKSRGGTWVAEDGTFFQVASDGNRMTLVTGEYYLTKEEA
                     ESRSLGISIPVGGPVRVQYNLLLQGNRITGSRSRGAIKTDACTIPEDSNEVTGELREA
                     DHMMTLRYPFMKYEARTVFTVFSLKNEACAGVEAVEKKEAEKKFFGPLPQGGIGVILG
                     GIHSYFPGGFSRMVFGWSGHLTIDQIEADSPAYAAGLRKGDEILAIDESPVINLSGSD
                     AIRRLRGEPGTEVALSVKHKNGETATVRMLRMKLN"
BASE COUNT          709 a         1314 c         1124 g          815 t
ORIGIN      
        1 aacgggaggc ggacagggcc acctgggtgt tctgctccac aaggagcaca gtggtcccgg
       61 ccaggacgac cttgcggatc gcctcgaaca tctcccggac gaccagggga gcaagcccca
      121 gcgagggctc gtccatcagg aggagccggg gcgcccccat caggccgcgt ccgatcgcga
      181 ccatctgctg ttcgccgccg gagagagtcc cgcagaactg gccctttcgg gaccgcaggt
      241 gggggaacag ggcgaagacg cgctcgagcc gctcgcgcac gatctccccc tccttctccc
      301 cgtaggcccc catttcgagg ttttcccgaa cggtcagcgg aggccacagc cgccgaccct
      361 cgggaatcat cgtcatcccc ttccggacga cggcgtgcgt cctctttccc tggatctctt
      421 cttcgagaaa gacgatcctc cccgccgtag gaggcaaaag cccgatgatg gatcgcagca
      481 gggtcgtctt ccccgctccg ttgggaccga ggatgcaggt catctcctcc cgacgaactt
      541 ggaaggacac ccgttcgagg acgtggacct ccccgtaccc cgccgagagg ttctccacct
      601 taagcatcgt ggcgctcccc gaggtacgcc tggaccacgc ccgggtcctt caccgcgtct
      661 tccggggcac cgtcgaagat cagttggccg aaattgagaa ccaggatccg gtgcgagagc
      721 cccatgatgg cgcgaaggtt gtgctcgatc atcagaaccg tgatcccccg ctcggcgtta
      781 agggaccgga cgaagggaat ggcgtcctcg acctccctcg ggttgaggcc ggcgagcacc
      841 tcgtcgagga gaagaacctt cggccgggtc gccagggccc gcgccatctc cagtcgcttt
      901 cgctgcggga gattcaacga ccgggcgggg agtgccgcca cggaggagag ccccgtgcat
      961 gcgagggcct cctcggcctc tctccggatc tccgcggcga tgggccgctc ctttttcccg
     1021 aagaccgcag ctaccttgac gttctcgaag acggtcattt ccgggaaggg acggaccacc
     1081 tggaacgtcc gggccattcc caggtgacag agttggtcgg tccgtaaagc tcccgtgagt
     1141 acgccgcgga accggatctc cccgccggtt gccgggagaa aaccgctcaa cagggcgaag
     1201 agggtcgtct tccctgcgcc gttgggcccg atgaccccga cgaactcacc ttcccgggcc
     1261 cggaaggaga gtgcggagac cgcgcgcaga ccaccgaaat ccttggagag gttggacaca
     1321 tcgaggagaa ttttcccccg ggggcccaca agggatgctg ccgtgcccgc cgaggcgggt
     1381 tccgtcaccg ggtcatctcc cgtgagatga ccatccggag gatttccgaa gttccttcgt
     1441 agatccggag gagccggagg tcgcggtaga atcgctccac ggccatccct ttcatgtagc
     1501 ccattccgcc gtggatctgg acagcgctgt ccgatgcgcg ccaagccgcc tccgtggcta
     1561 atactttgac catcgccgcc tccttggtca cacggtcgcc gcggtccatc ttccacgccg
     1621 cgttgtaaac ggcgttccgc accgcgtagg cgtcgatcgc catattcacg agcatccact
     1681 ggatcgcctg ccggttgatg atcggttcgc cgaacgtcac ccgcatggcc gcgtaatccc
     1741 tggagaggtc gatgagcttc tcgagggacc ccagggcaca cgcccccatc gtgagccgcc
     1801 ccttgtcgag gatcttcatg gcgatcttga acccctcgcc ctcgcggccg aggaggttct
     1861 ccgccggcac ctcgcaatcg tcgaagacca gctcgcaggt gtgggagccg cggaacccca
     1921 tcttccgttc gatcgtcccc accgagaacc ccttgaatcc cttctccacg agaaaggccg
     1981 agatcccctt tgtccccttc cccttgtcgg tgatggccat caccgtggag acgtccgcga
     2041 tgtccccgtt ggtgatgaag tgcttcatcc cgttgaggat aaacttgtcg cctttgcgca
     2101 ccgccgtggt gcggatcgcc gatgcgtccg aacccgcgtt cggctccgtg agggcgaatg
     2161 caccgatcat ctcgcccgtg gcgagcttcg gcaggaactt ccgccgttgc gcttccgtac
     2221 cggcgatgac gatccccatc gagccgatcc cgttgttcgt cccgatgcgg gtccggaaac
     2281 aggcgttcgt ccgcgacaac tcctccatcg cgacgcactg gccgaggacg ctcatgccga
     2341 acccgccgta gtcggccggg attgccagtc cgaagagccc cagttccgcc atcttggcca
     2401 ccgtctcgtc cgggatgcga tcctcctcct ccaccttctc ggagatcggc tccagttcct
     2461 tgtcgacaaa tttccggagc gtatctcgga acatcacaag ctcttcaggc aaggtgaaat
     2521 ccaccgcgca tcctccagtg ggacatggaa tgggaattcc agcatctctt caatcggagg
     2581 gattataccc tcccgtgtct attatcggca tcgtgaattt caaggctgtg aagtaaattg
     2641 tgaagcagac cctgcctacc aggtacgaac aggtacaaag aagggggagc cggtctcccg
     2701 tttccccctt ttctttcggc ctatttccta ctcctgccta atgacttctg tgctttctgg
     2761 tccgtctcaa aatcccgtgt ccgcaaggac gtgagggttc aagtccctcc tccggcacca
     2821 tttacaataa agaggagcgt cgggtccggc tcagttcagt ttcatccgaa gcatgcgcac
     2881 cgttgcggtt tccccgttct tatgcttgac cgaaagagcg acctccgtcc ccggctcccc
     2941 cctcaaccgg cgaatggcgt cgctgccgga aaggttgatg acgggggatt cgtcgatggc
     3001 gaggatctca tcccccttcc gaagcccggc ggcgtaagcc ggtgaatctg cctcgatctg
     3061 gtcgatcgtc aaatggccgg accatccgaa caccatcctg gaaaaccctc cagggaagta
     3121 cgaatgaatc cccccgagga tgacaccgat gcccccttgc ggcagggggc cgaagaactt
     3181 cttttccgcc tccttcttct cgacagcttc gactcccgcg caggcttcat ttttcaagct
     3241 gaatactgtg aacacagtac gggcttcgta tttcatgaag ggatatcgca gggtcatcat
     3301 atggtcggct tcgcgcaatt cgccggtgac ctcgttactg tcttcaggaa tggtgcatgc
     3361 gtcggtcttg attgctccac gactccgtga accggtgatc ctgttcccct ggagcaggag
     3421 gttgtattgc acgcgaacag gtccgccaac tggtatcgaa atccctaacg atctcgattc
     3481 ggcttcttcc tttgtgaggt aatattcacc ggttaccagc gtcatccgat tcccgtcaga
     3541 ggccacttgg aagaatgtcc cgtcttcggc cacccaggta ccgccgcgcg acttgagtct
     3601 ccccgtttgc tccatccgat actcgagttt ggtgatctcg tccttgactc gttgggtgtc
     3661 ctccgccttc ggcgtcaatg cgagatattt ctggtaactt tcgatggctt ccttcaactg
     3721 cccgatcttc gactggacgg atgcgaggtt gtaccaggcc gccgccatgt tcggcgcgat
     3781 ttcgatcgct ttcttgaact cgacggcggc attcgacagg tcatcctccg tcttggccat
     3841 ttcgaccgcc gccatcccgc gcaccatata tctgcgggcg tcttcctccg ggctctggcc
     3901 gaaagcggga accgccagcg ccaacatcag caatgccgtc atcgcctttt cgtatcgatg
     3961 ag
//
LOCUS       DASVJL010000232         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1348618, whole genome shotgun sequence.
ACCESSION   DASVJL010000232 DASVJL010000000
VERSION     DASVJL010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348618"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VF357_04710"
     CDS             complement(<1..847)
                     /locus_tag="VF357_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX7523479.1"
                     /translation="MLLGNLGSLPYFLPELAVTATILLLVVLHVVSKRKTTSAPAFLS
                     LLGVGAAILLSGVSPAGSGKAIFEGMAAYDAFAVFFKVLIAFATLVVILMSMNSEELA
                     GRSKAEYFIFLLSTLLGMFLLSSATDIVMLYLSLELVSIPSYLLAGYLKGKEHSTEAA
                     MKYVVFGATASGVMIYGFSLLFGMTGSTQIAEIGRALAAGKAALPTVLAAVMVAVGFG
                     YKIAAVPFHMWSPDVYEGAPTPATAFFSVAPKAAGFAVLVRFYYTVFAGPDAAAGMWR
                     ITSGMD"
     gene            complement(849..>1593)
                     /locus_tag="VF357_04715"
     CDS             complement(849..>1593)
                     /locus_tag="VF357_04715"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX7523480.1"
                     /translation="SVILAGVLLKMGAYGLMRISFPIFPDVTRWFAMPMAVIGVVNIV
                     YGALCALAQSDLKKMVAYSSVSHMGFVLLGMAALTPLGMAGASMQMFSHGLITAMLFF
                     LVGVVYDRAHHRQIDGFGGLGAVVPIYTGFISFAFFASLGLPGMSGFIAEQMVFLGSF
                     EAFRPIVIVAAVGIVFVAAFHLWSLQRVFLGPLNPKYATMEEINAREIFCLAPLGILV
                     LIIGVWPMPMLNLLNASAVRLVDLVKAVI"
BASE COUNT          334 a          502 c          549 g          208 t
ORIGIN      
        1 agtccatccc ggaggtgatc cgccacatcc ccgccgcggc gtccgggccg gcgaagaccg
       61 tgtagtagaa gcggacgagc accgcgaacc ccgccgcctt cggggcgacc gagaagaagg
      121 ccgtcgccgg cgtgggcgcc ccttcgtaca cgtcggggct ccacatgtgg aacgggacgg
      181 cggcgatctt gtagccgaac cccacggcga ccatcacggc ggccaggacc gtgggcaggg
      241 ccgccttgcc cgccgcgagc gcccgcccga tctccgcgat ctgcgtggag ccggtcatgc
      301 cgaagagaag ggagaagccg tagatcatca cacccgacgc cgtcgcgccg aacacgacgt
      361 acttcatcgc cgcctcggtg gagtgttcct ttcccttgag gtacccggcc agcaggtacg
      421 acgggatgga gacgagctcc agcgacaggt agagcatcac gatgtcggtt gcgctggaga
      481 ggaggaacat cccgaggagg gtggacagga ggaagatgaa gtattccgcc ttgctgcggc
      541 cggccagctc ttcgctgttc atcgacatga ggatgacgac gagggtcgcg aaggcgatca
      601 ggaccttgaa gaagacggcg aaggcgtcgt acgccgccat cccctcgaag atcgccttcc
      661 cgctccccgc gggcgagacc cccgagagga ggatcgcggc gcccaccccg aggagggaga
      721 gaaacgcggg ggcggaggtc gtcttccgct tcgatacgac gtgcaggacg acgagcagaa
      781 ggatcgtcgc cgtgacggcg agctccggca ggaagtacgg caggctgccg aggttgccga
      841 ggagcatatc agatcaccgc cttcacgagg tcgaccaggc gcaccgcgga ggcgttcagc
      901 aggttcagca tcggcatcgg ccagacgccg atgatcagca cgaggatccc gagaggcgcc
      961 aggcagaaga tctcccgggc gttgatctcc tccatggtgg cgtacttcgg gttcagcgga
     1021 ccgaggaaaa ccctctgcag cgaccacagg tggaaagcgg cgacgaagac gatgccgacc
     1081 gccgccacga tcacgatggg ccggaacgcc tcgaaggagc cgagaaatac catctgctcg
     1141 gcgatgaacc cggacatccc cggaagaccg agggaggcga agaaggcgaa ggagatgaat
     1201 ccagtgtata tgggcaccac ggcgcccagc cccccgaagc cgtcgatctg gcggtggtgg
     1261 gcccggtcgt agaccacgcc gaccaggaag aagagcatgg ccgtgatcag gccgtgggag
     1321 aacatctgca tcgatgcgcc cgccatcccg aggggggtga gcgccgccat gccgagcagg
     1381 acgaacccca tgtggctgac ggaggagtag gcgaccattt tcttgaggtc cgactgcgcc
     1441 agggcgcaca gcgcgccgta gacgatgttc accaccccga tcacggccat cggcatggcg
     1501 aaccatctcg tcacgtcggg gaagatcggg aaggagatcc gcatcaggcc gtacgccccc
     1561 atcttcagca ggacgcccgc gaggatgacg gag
//
LOCUS       DASVJL010000233         1685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_35427, whole genome shotgun sequence.
ACCESSION   DASVJL010000233 DASVJL010000000
VERSION     DASVJL010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35427"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..882)
                     /locus_tag="VF357_04720"
     CDS             complement(49..882)
                     /locus_tag="VF357_04720"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX7523481.1"
                     /translation="MKKALLVGINRYPDPRNELKGCVNDVRQMAETLKSRYGFPGDGN
                     MRILTDARATTKAILDGLAWLTAGASPGDSLVFHYSGHGSQVPDRNGDETTDRLDEIL
                     CPYDLDWDHPLTDDDLAAACADVPQGALLTVILDCCHSGTGLRDFAPDRHRFLPHPET
                     MHQTSPRPRRPARRFGVSVTRTNAVLIAACRDDQTSADAWIDGGYHGAHTYHLCRTLG
                     NGTRDLTYRALVSSTGTALSRAGFDQVPQLEGPDRLLVDPVFRLRLPLPKIAGFDIVS
                     N"
     gene            complement(1181..1597)
                     /locus_tag="VF357_04725"
                     /pseudo
     CDS             complement(1181..1597)
                     /locus_tag="VF357_04725"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
BASE COUNT          263 a          486 c          663 g          273 t
ORIGIN      
        1 cgcacgcgca cgggatgcga gtttcattgg aatggggcgt acgccggatt aatttgaaac
       61 aatatcaaag ccggcaatct tcggcaaggg aaggcggagg cggaacaccg gatccacgag
      121 caggcggtcg ggcccctcga gctgggggac ctggtcgaag ccggcgcggg agagggcggt
      181 gccggtggag gaaacgaggg cgcggtaggt gagatcgcgg gttccattgc cgagggtgcg
      241 gcaaaggtgg tacgtgtgtg cgccgtggta gccgccgtcg atccaggcgt cggccgaggt
      301 ctggtcgtcc cggcaggcgg cgatcagcac tgcgttggtg cgggtgacgc tgacgccgaa
      361 ccggcgggcg ggtcggcgtg gacgcggcga agtttggtgc atcgtctccg ggtgcgggag
      421 gaagcggtgg cggtcggggg cgaagtcgcg caggccggtg ccggagtggc agcagtcgag
      481 gatgacggtg aggagggctc cctgcgggac gtcggcgcag gcggcggcga ggtcgtcgtc
      541 cgtgagcggg tgatcccagt cgaggtcgta cgggcagagg atctcgtcga gccggtcggt
      601 cgtctcgtcg ccgtttcggt cgggcacctg ggagccgtgg ccggaatagt ggaagacgag
      661 ggagtcgccc ggggaggccc ccgcggtgag ccaggcgagc ccgtcgagga tcgccttcgt
      721 ggtggcgcgg gcgtcggtga ggatgcgcat gttcccgtcg ccggggaagc cgtaccggct
      781 tttcagcgtt tccgccatct ggcggacatc gttgacgcac cccttcagct cgttgcgcgg
      841 atccgggtac cggttgatgc cgaccagaag cgctttcttc atcgtcgctc cctccctggg
      901 ccgcagcgcg gcttgttgcg gggaggaggg agcaaggggc gggccggtcg cgtgcgaggg
      961 cgctcgggtg ggcacggcgg ggcggcggga ccggaaaacg tgcgtgcggg aaggagttgc
     1021 gggagtgaaa ccggaccgag agagcgtgcg gagaccggag gggcgcgcgg cgagaggccg
     1081 gttatcgaac cttgtgtccg ggaactggtc ggtggcccgc ggctacgacg agccccccct
     1141 gccccgttgg cccgccttca acgtcggacc agccgcgcca tcaccccgcg gcgagcaact
     1201 cccggaagaa ggcgacggtg cgggtgagac cgtcgcgcag cgggatcgtc ggctcccagt
     1261 gaagcttcgc cttcgccagg gtgatgtccg ggcaacggcg gaccgggtcg tcttgcggaa
     1321 gcggtttgaa aacgatctcc gacttggagc cggtgagctc cttgaccatc gtcgccagct
     1381 cgaggatcgt gaactcgccg gggttcccca ggtttaccgg ccccactatc tcgtcctggt
     1441 ccatcatcgc gatcatcccg gtgacaaggt cgtccacgta gcagaaggag cgcgactggc
     1501 tccccgttcc gtacagcgtg atcggctccc ccttcagcgc ctggacgatg aagttcgaca
     1561 ccacgcggcc gtcgttcggg tgcatccgcg ggccgtacgt gttgaagagg agacatcagg
     1621 gcctgcaggg atgctggaaa tggatgccag cggctagttc gtgatcgtcg ccttgagagg
     1681 ccgca
//
LOCUS       DASVJL010000234         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1349013, whole genome shotgun sequence.
ACCESSION   DASVJL010000234 DASVJL010000000
VERSION     DASVJL010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349013"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF357_04730"
     CDS             complement(<1..252)
                     /locus_tag="VF357_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX7523482.1"
                     /translation="MNVAILADIHANFDALSAVAAELAFRRPDRIYHLGDLVGYNAQP
                     EECIRWAMANTAAGVSGNHDAVACGRATGRLFHAAARKAA"
     gene            complement(249..1541)
                     /locus_tag="VF357_04735"
     CDS             complement(249..1541)
                     /locus_tag="VF357_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523483.1"
                     /translation="MSGSPLSGKTVYFPAMSEAGVRAIAAAFRSVGIDAKSMPPSDEE
                     TMVLGGRHSSGEECLPLKVTLGDALKLLVRGEIAPDRMAIFMPTSDGPCRFGQYGPYI
                     KAVLTEMGFDGVTVVSPTCEDGYSGVGEHADELLRTAWRAMIAGELLTKLLLRIRPYE
                     LEKGASDAAYQAALSDLCVTVESRGKNPKERMARLKESLVRGRRMMRDVPADFSDPRP
                     LIGIVGEIFCRLNTFSNDDVVRKVEEAGGECWISDISEWLWYTNAAHLHLLKRFRKHL
                     TFEMFGARLKWHFQHKDERELHSVFGDDFRGYEEADDIRDVLDLARPYLVPEGTLGEM
                     LLSVAKTVYLHRKGADGVLDINPFSCMNGIVSEAIYPRLSRDCDGLPVRVFYFDGTRA
                     DRRYELDIFMDLVREYSARKRTVRALPLGLGRAAAVNA"
BASE COUNT          262 a          570 c          541 g          271 t
ORIGIN      
        1 agccgccttc cgcgccgcgg cgtggaagag ccggcccgtg gcccggccgc acgccacggc
       61 gtcgtggttc ccggatacgc ccgccgccgt gttcgccatc gcccaacgga tgcactcttc
      121 cggctgggcg ttgtatccga cgaggtcccc gaggtgatag atccggtccg gccggcggaa
      181 ggcgagctcc gccgcaaccg ccgagagggc gtcgaagttc gcgtggatgt ccgcgaggat
      241 ggcgacgttc aagcgttgac ggccgcagcc ctccccagcc cgagcgggag cgcccggaca
      301 gtccgtttcc tggccgaata ttcccggacg aggtccatga agatgtcgag ctcgtaccgc
      361 cggtcggccc gcgtcccgtc gaagtagaag acccggacgg gcaggccgtc gcaatcgcgc
      421 gagaggcgcg ggtagatcgc ctcggacacg atcccgttca tgcaggagaa cgggttgatg
      481 tccagcaccc cgtcggcccc cttccggtgc aggtagaccg tcttcgcgac cgaaaggagc
      541 atctccccca gcgttccttc gggaacgaga tacggccgcg cgaggtcgag cacgtcgcgg
      601 atgtcgtcgg cctcctcgta gccgcggaaa tcgtcaccga agacggagtg aagttcccgt
      661 tcgtccttgt gctggaagtg ccacttcagg cgcgcgccga acatctcgaa ggtcaggtgc
      721 ttccggaagc gcttcagcag gtggaggtgg gcggcgttgg tgtaccagag ccattcgctg
      781 atgtcggaga tccagcactc tccccccgcc tcctcgacct tgcggacgac gtcgtcgttg
      841 ctgaacgtgt tgaggcggca gaagatctcc cccacgatcc cgatcagcgg ccgcggatcg
      901 gagaagtccg ccgggacgtc gcgcatcatc ctccgtcccc ggacgaggga ttccttgagg
      961 cgagccatcc gctccttcgg gttcttcccg cgggattcga ccgtgacgca gaggtcggac
     1021 agcgccgcct ggtacgccgc gtcggacgcc cccttttcca gttcgtaggg ccggatccga
     1081 aggagaagct ttgtcagcag ctcgccggcg atcatcgccc tccacgccgt gcgaagcagt
     1141 tcgtcggcat gctccccgac cccgctatac ccgtcctcgc acgtcgggga gacgaccgtg
     1201 acgccgtcga accccatctc cgtcaggacc gccttgatgt aggggccgta ctggccgaac
     1261 cggcacggtc cgtccgaggt gggcatgaaa atcgccattc gatcgggggc gatctcgccg
     1321 cgcaccagga gcttcagcgc gtcccccagc gttaccttga ggggaaggca ctcctctccc
     1381 gaggagtggc gccccccgag gaccatcgtc tcctcgtcgg agggcggcat cgatttcgcg
     1441 tcgatcccca cggagcggaa cgcggctgcg atggcgcgga ccccggcctc cgacatcgcg
     1501 ggaaagtaga ccgtcttccc ggacagcggc gaaccgctca ctgctgcgcc catgggcgga
     1561 gcaccccctt gctgtcgagg taggcctcgc accgggtcac gtacccggcg tcgttgccgt
     1621 ggccgtcgaa ctgcagcgtc aggt
//
LOCUS       DASVJL010000235         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_317773, whole genome shotgun sequence.
ACCESSION   DASVJL010000235 DASVJL010000000
VERSION     DASVJL010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317773"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VF357_04740"
     CDS             complement(<1..247)
                     /locus_tag="VF357_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523484.1"
                     /translation="MKPGVPILLGLLILSPAAGRAAEGYQVYESTAASVNGEVLFVSD
                     VVREACFLRCAAMPGTVGEILSPRGVRDRLVLDTLALQ"
     gene            complement(244..1068)
                     /gene="nadC"
                     /locus_tag="VF357_04745"
     CDS             complement(244..1068)
                     /gene="nadC"
                     /locus_tag="VF357_04745"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX7523485.1"
                     /translation="MISRHLYEEIVRAALREDAPFGDPFGEAFQGEASGLFLAGGDGV
                     LCGGPVASEVFRQVDPSVSVSFSPEGSRVARGDRVGKASGPAGSLLRAERVALNFLQR
                     LSGVATATSLYASRIAAHGTKLLDTRKTTPLLRVLEKYAVRVGGGTNHRFSLSDGILI
                     KENGIRAAGGIAPAVAAARSAAHHLLRVEVEAETIPDVEAAVAAGADAVLLDNMPPSM
                     VREAVARFGGIVFLEASGGIHLGNVEEYARTGVAAIAVGAITHSAPALDISFELST"
     gene            complement(1065..>1839)
                     /locus_tag="VF357_04750"
     CDS             complement(1065..>1839)
                     /locus_tag="VF357_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7523486.1"
                     /translation="EEYRFNEASSAFYQFVWHEFCDWYLEMIKPVLSPEAGEVERQVQ
                     RAVLIRIYETILALGHPFIPFLTEELWHALPGKRGLLYDRPYPVVEPGATDENVEDEM
                     AHLMEVIRAVRNIRSELNVPPGKKVEVRLKGQMEVQTFLRDHEEIVRRLARADRVTYV
                     DPDYIPVKDATAVVNDIEVCLPLEGLIDFGQEAKRLAKEVEKAKAEYARVAGQLGNAR
                     FTAKAPPDIVDALRDREKTLEQKIAKLGKNFELVNRYLA"
BASE COUNT          296 a          657 c          605 g          281 t
ORIGIN      
        1 cctggagcgc cagtgtgtcg aggaccaggc gatcccgcac cccacggggt gacaggatct
       61 cccccacggt cccgggcatc gccgcgcacc ggaggaagca cgcctcccgc acgacgtcgg
      121 agacgaagag cacctcgccg ttcaccgacg cggcggtgga ctcgtagacc tggtacccct
      181 cggccgcgcg ccccgccgcg ggagaaagga tcaggaggcc caggaggatg ggaacgccgg
      241 gtttcacgtc gacagttcga aggagatgtc gagggcgggg gcggagtggg tgatcgcacc
      301 gacggcgatg gcggccaccc ccgttcgggc gtactcctcg acgttcccga ggtggatccc
      361 cccggacgcc tcgaggaaga cgatcccgcc gaagcgcgcg accgcctccc ggaccatcga
      421 cggcggcatg ttgtcgagga ggaccgcgtc ggcccccgcc gcgaccgccg cctccacgtc
      481 cggaatcgtc tccgcctcga cctcgacgcg gagcaggtgg tgcgccgccg aacgcgcggc
      541 ggcgaccgcc ggggcgatcc ccccggccgc gcggatcccg ttctccttga tcaggatccc
      601 gtccgaaagg gaaaaccggt ggttcgtccc gccccccacc cgaacggcgt acttctcgag
      661 gactcgaagc agcggcgtcg ttttccgcgt atcgagcagc ttcgtgccgt gggcggcgat
      721 ccgcgaagcg tagagggacg tcgcggtggc cacgcccgag agccgctgga ggaagttgag
      781 ggccacccgc tccgctctca agagagagcc ggcaggaccg gacgctttcc cgacccggtc
      841 gccgcgggcc acgcggctcc cttcggggga gaaggaaacg gaaacggacg ggtcgacctg
      901 gcggaacacc tccgacgcca ccggcccgcc gcacagcacc ccgtcacccc ccgccaggaa
      961 aaggcccgac gcctccccct ggaacgcctc tccgaaagga tcgccgaacg gggcgtcctc
     1021 tcgcagagcc gcgcggacga tctcctcata caggtgccgg gagatcacgc caggtatcgg
     1081 ttcaccagct cgaagttctt cccgagcttg gcgatcttct gttccagcgt tttctcgcgg
     1141 tctcgaaggg cgtcgacgat gtcggggggg gccttggcgg tgaaccgcgc gttccccaac
     1201 tgcccggcga ctcgggcgta ttccgccttc gccttctcca cctccttggc cagacgcttc
     1261 gcttcctgcc cgaagtcgat gagaccctcc agggggagac agacctcgat gtcgttcacc
     1321 accgccgtcg cgtccttcac agggatgtag tcggggtcga cgtacgtcac ccggtcggcc
     1381 cgggcgaggc gccggacgat ctcctcgtgg tcccgcagga acgtctgcac ctccatctgg
     1441 cccttcaggc gaacttcgac cttcttccca ggcgggacgt tcagctcgct gcggatgttt
     1501 cgaaccgccc ggatgacctc catcaggtgc gccatctcgt cctcgacgtt ctcgtcggtc
     1561 gcgccgggtt cgacgacggg gtacggccgg tcgtacagca gcccccgctt ccccggcagg
     1621 gcgtgccaca gctcctcggt gaggaagggg atgaacggat ggccgagggc caggatcgtt
     1681 tcgtagatcc ggatcaggac ggcccgctgc acctgccgct ccacctcgcc cgcctcgggg
     1741 gatagcacgg gtttgatcat ttcgaggtac cagtcgcaga actcatgcca gacgaactgg
     1801 tagaaggcgg agctcgcctc gttgaaccgg tactcctcg
//
LOCUS       DASVJL010000236         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1349208, whole genome shotgun sequence.
ACCESSION   DASVJL010000236 DASVJL010000000
VERSION     DASVJL010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349208"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VF357_04755"
     CDS             complement(<1..885)
                     /locus_tag="VF357_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX7523487.1"
                     /translation="MESVERTEADRNGRGYLSERGWRSWAFTLDHKRIGVMYLWTTIF
                     FFLVGGVFALLLRLELLSPQARYFSNHTNNIFFTLHGSMMIFLFIIPAIPSGLGNFFI
                     PLQIGARDVAFPRLNLAAYWVFAAGFLVVIGSLVAPMDTGWTFYTPYSAKSAAAVTTL
                     SLGLFLIGMSSILTGLNFIVTIHKLRAPGMTWHRMPLFLWGMYATSIIQVIATPVVGV
                     TFLLLAAERVLGVGFYDPSKGGDPVLFQHFFWFYSHPVVYVMILPAMGIISEVIPVFS
                     RKPIFGYNAIAYSSLAIAF"
     gene            complement(899..1849)
                     /gene="coxB"
                     /locus_tag="VF357_04760"
     CDS             complement(899..1849)
                     /gene="coxB"
                     /locus_tag="VF357_04760"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX7523488.1"
                     /translation="MDNVLAFGAASSQAGRVDALFVMILVIGVFFFFLTQGILIYFAV
                     KYRRRRPDRDNDTPAITGNPLLEFLWILIPSLVVLVIFYYGWRVYTDLRIPTAGATEV
                     HVNARQWMYEIKYPDGRTAINEIRVPAGKPVKFILSASDVLHGFYLPDFRVKMDMIPG
                     RVTTLWVQPDRPGSYQIFCTVYCGTGHSNMLARLIVMPPREYAEWVEHGGRKGGEAGG
                     KEPLHERGERVVKGAGCLNCHAVEGKEKIGPNFRGLYGSKVPLEGGKSVTADEEYLRE
                     SIVEPGAKVVKGYPNVMPTFKASMLPDDVRAAVEYLKTLK"
     gene            complement(1849..2652)
                     /locus_tag="VF357_04765"
     CDS             complement(1849..2652)
                     /locus_tag="VF357_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7523489.1"
                     /translation="MLFAFLLLLPCVALAADPQDAILQEVGVDEKPGAAIPSDLPFTD
                     ASGKPVRLADYLGDGPVLLTLNYYTCPMLCPLTFRSLAATMAQLKGFSLARDYRVVTV
                     SIDPDEIPEIARAKANETHALMPGVREADARWPFLYGSAGSIRRLTESVGYRYKKVGS
                     EFAHPAVMIVLSPGGTVSRYLYGVEIDPRDLKLALIEASEGKIGASSAGNALLMYCFR
                     YDPVGKRYMLYALNIMKAAGAVTVVLLAALLIVLWRRHERATKPRGEGE"
BASE COUNT          532 a          895 c          905 g          463 t
ORIGIN      
        1 gaaggcgatc gccagcgacg agtaggcgat ggcgttgtac ccgaagatcg gcttgcggga
       61 aaagaccggg atcacctcgg atatgatccc catcgccggg aggatcatca cgtagacgac
      121 ggggtgcgaa tagaaccaga agaagtgctg gaagaggacc gggtcgcccc ccttggacgg
      181 gtcgtagaac cccaccccga gcacgcgctc cgcggccagc aggaggaacg tgactccgac
      241 caccggcgtg gcgatcacct ggatgatgct cgtggcgtac atcccccaga ggaagagcgg
      301 catccggtgc catgtcattc ccggggcccg caatttgtgg atcgtgacga tgaagttcag
      361 gcccgtgagg atcgacgaca tgccgatgag gaagagcccg agggagaggg tcgtcaccgc
      421 ggcggccgac ttcgccgagt acggcgtgta gaaggtccac cccgtgtcca tcggggccac
      481 cagcgacccg atgaccacga ggaacccggc ggcgaagacc cagtaggccg cgaggttcag
      541 ccgcgggaag gcgacgtcgc gggctccgat ttggagaggg atgaagaagt tcccgagccc
      601 ggaggggatc gccgggatga tgaagagaaa gatcatcatc gacccgtgga gggtgaagaa
      661 gatgttgttg gtgtggttgc tgaaatatct tgcctggggg gagagcaact ccagccgcag
      721 cagcagggcg aacacgccgc cgaccaggaa gaagaagatc gtcgtccaga ggtacatcac
      781 cccgatccgc ttgtggtcga gggtgaacgc ccaggaacgc cacccccgct ccgacaggta
      841 ccccctgccg ttccggtccg cttcggtccg ctctacgctt tccatcccat tcaccgcctc
      901 acttcaacgt cttcaggtac tcgacggccg cacggacgtc gtccggcagc atcgaggcct
      961 tgaacgtcgg catcacgttg gggtacccct tcacgacctt ggcgcccggc tccacgatcg
     1021 actcccgcag gtactcctcg tcggccgtca cgctcttccc cccttcgagg gggaccttcg
     1081 aaccgtagag cccgcggaag ttggggccga tcttctcctt cccctcgacg gcgtggcagt
     1141 tgaggcagcc ggctcccttc acgacgcgct ctccccgctc gtgaagaggc tctttccccc
     1201 ccgcctcccc ccctttgcgc cccccatgct ccacccactc ggcgtattcg cgcgggggca
     1261 tcacgatcaa ccgggccagc atgttcgagt gtccggtccc gcagtacacc gtacagaaga
     1321 tttgatagct gcccggccgg tccggttgca cccaaagggt ggtcaccctg ccggggatca
     1381 tgtccatctt cacccggaaa tccggcaggt agaatccgtg gaggacgtcg gacgcggaga
     1441 ggatgaactt caccggcttg cccgccggga cgcggatctc gttgatcgcc gtccgtccgt
     1501 cggggtattt gatctcgtac atccactgcc gcgcgttgac gtgcacctcc gtcgccccgg
     1561 cggtggggat ccgcaggtcg gtgtagaccc gccagccgta gtagaagatt accaatacga
     1621 cgagggacgg gatcaggatc cacaggaact cgagcaaggg gttgcccgtg atggcggggg
     1681 tgtcgttgtc ccggtccggg cggcgacggc ggtacttcac ggcgaaatag atcagtattc
     1741 cttgggtgag aaaaaagaag aatacgccga tgacgaggat catcacgaag agcgcgtcga
     1801 cccggcccgc ctggctggag gccgcaccga acgcaaggac gttgtccatc actccccttc
     1861 accccgtggt ttcgtcgccc tttcgtgacg ccgccagaga acgatgagaa gcgccgcgag
     1921 aagaaccacc gtgaccgcgc cagccgcctt catgatgttg agtgcgtaca gcatgtacct
     1981 cttcccgacc gggtcgtacc ggaaacagta cattagaagc gcgtttcccg cggaagatgc
     2041 gccgatcttt ccttccgacg cctcgatcag ggcgagtttc aggtcccggg gatcgatctc
     2101 gaccccgtac agataccggg agaccgtgcc gccgggggaa aggacgatca tcacggccgg
     2161 atgggcgaac tccgagccga ctttcttgta gcggtaaccg acgctctccg tcagccggcg
     2221 gatcgatccg gcgctcccgt acaggaacgg ccaccgtgcg tccgcttccc gcacgcccgg
     2281 catcaacgcg tgcgtctcgt tcgccttcgc gcgggcgatc tccgggatct cgtccgggtc
     2341 gatgctcacc gtgacgacgc ggtagtcccg cgcaagggag aatcccttca actgcgccat
     2401 cgtggcggcg aggctccgga acgtcagagg gcagagcatc gggcaggtgt agtagttgag
     2461 ggtgaggagg accgggccgt cgccgagata gtcggccagt cggaccggtt tcccggacgc
     2521 gtcggtgaac ggaaggtcgc tcggaatcgc cgcgccgggc ttctcgtcca ctccgacctc
     2581 ctgcaagatc gcatcctgcg ggtcggccgc gagggcaacg cagggaagga gcagcaggaa
     2641 ggcgaacaac acgacggcga gacgccttat ccatcctggc cgggacccgt tcacgggaga
     2701 acgagcggct ggtcgggcga ggggacctgc gcgagccacc cgagcttctc tttcagcgtc
     2761 ccggcgaatt cgagggagac ggactcctcg ccgtg
//
LOCUS       DASVJL010000237         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_131325, whole genome shotgun sequence.
ACCESSION   DASVJL010000237 DASVJL010000000
VERSION     DASVJL010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131325"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..564
                     /locus_tag="VF357_04770"
     CDS             31..564
                     /locus_tag="VF357_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX7523490.1"
                     /translation="MKKGPSRAKESAVMKGLKQALRNEIDGAEFYRMAAGSARRDSVR
                     QMFRFLMEEEERHREAIVNQMQRLAEGKGMKFERSASGKKALAKFKSPLLTDDLVKEG
                     RQVEGEVAALSIGMTLEKRAIAQFIVLRKTVAGDAPAEKVFSDLVLWEQDHLDVLTRQ
                     YDQMREIFWEEARFWPF"
     gene            601..1110
                     /gene="tadA"
                     /locus_tag="VF357_04775"
     CDS             601..1110
                     /gene="tadA"
                     /locus_tag="VF357_04775"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX7523491.1"
                     /translation="MSSSSRRVRGPGVAEPAREAWMRAALREAEKGAAAGEIPVGAVV
                     ISAEGKILARAHNRSVTANDPTAHAEILALRGAARRTGNYRLTGCRLVVTLEPCPMCA
                     GAAVVARVEEIIYGTPDPKGGAVSTLYHIASDPRLNHRAAVTSGVLAEECAALLTSFF
                     RSRRSAPPR"
     gene            complement(1124..2545)
                     /gene="cls"
                     /locus_tag="VF357_04780"
     CDS             complement(1124..2545)
                     /gene="cls"
                     /locus_tag="VF357_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEX7523492.1"
                     /translation="MILALNIAGYLFAFFLIPRVILERRHPSATLAWMLGIALLPLIG
                     IPLYFLIGVHRVRRHIRAKIAAVAPVASSLSHRLRPEELPTDVGERCDRVLLAAGTPP
                     PTEGNRVTFLRGGDEAYEAVLSLIGSARDHLHAQFFILDVDPVGRRFIQALAARARAG
                     IRVRLLLDAVGSWRALRRTVRPLRDAGGEVAAFMPAFPLHRRWSAHLRNHRKLLIADG
                     RKAFTGGMNIGKKYMGPKVAKEQWRDLAVVIAGPALPDLQALFLDDWAFATGETLPPG
                     DLFPPPARFEGGNPPPCALQIATSGPDRNTRPIYEGVFAVFAAARRRLWIETPYFVPD
                     DGIGAALRNAALRGVDVRLIVPGTSDLRIVSLAGRSYFDEMMEAGVRIHLYRPTNLHS
                     KVLVVDDDVGVIGSPNVDMRSFFLNFELGVFLYGRPQIEALADGFLKDLERSEPVDPV
                     RFARRPRSLQLLEDTCRIFSPLF"
     gene            2643..2735
                     /locus_tag="VF357_04785"
     tRNA            2643..2735
                     /locus_tag="VF357_04785"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2678..2680,aa:Ser,seq:cga)
BASE COUNT          487 a          907 c          976 g          409 t
ORIGIN      
        1 catcttaatg caggaggagg atccccccaa atgaaaaagg gtccctcgcg agcgaaggag
       61 agcgccgtca tgaaggggct gaagcaggcg ttgcgcaacg agatcgacgg cgcggagttc
      121 taccggatgg cggcgggaag cgcccgacgc gactcggtcc gccagatgtt ccggttcctg
      181 atggaggagg aggagcgcca ccgcgaggcg atcgtgaacc agatgcagcg gctggccgag
      241 ggaaaaggga tgaaattcga gcggagcgcg tccgggaaga aggcgctcgc gaagttcaaa
      301 agtcccctgc tcaccgacga cctcgtgaag gagggccggc aggtggaggg ggaggtggcg
      361 gcgctctcca tcgggatgac gctggagaaa cgggcgatcg cccagttcat cgtcctgcgc
      421 aaaacggtcg ccggcgatgc cccggcggaa aaggtcttct ccgacctcgt cctatgggag
      481 caggaccacc tggacgtcct gacgcgtcaa tacgaccaga tgcgggagat cttctgggag
      541 gaggcgcgct tctggccgtt ctaagtgtgg gcgagcggac gccgggggag cggccaccca
      601 atgagtagtt cctcccggcg cgtacgtggt ccgggggtcg cggaacctgc gagggaggcg
      661 tggatgcggg ccgcgctccg ggaagcggaa aagggggccg ccgccggcga gatccccgtg
      721 ggcgcggtcg tgatctctgc ggaagggaag atcctcgcgc gggcgcacaa ccggtccgtc
      781 accgcgaacg accccaccgc ccatgccgag atcctcgcct tgcgcggagc ggcacgcagg
      841 accggcaact accggctgac cggctgccgc ctcgtcgtga cgctcgagcc gtgcccgatg
      901 tgcgccggtg ccgcggtggt cgcgcgggtg gaggagatca tctacggcac gcccgacccg
      961 aaggggggcg ccgtctccac cctctatcac atcgcgtccg atccgcgcct caatcaccgc
     1021 gccgccgtca cttccggcgt cctcgcggag gagtgcgccg ccctcctcac gtccttcttc
     1081 cgctcgcgcc gctctgcgcc gccccgataa gagatccgcg gggtcagaac aggggggaga
     1141 agatccggca ggtgtcctcg agcaactgca gggagcgcgg ccgccgcgcg aaccggacgg
     1201 ggtcgaccgg ctccgacctc tcgaggtcct tgaggaaccc gtccgccagc gcctcgatct
     1261 gcgggcggcc gtagaggaac accccgagct cgaaattcag gaagaagctc cgcatgtcga
     1321 cgttgggtga tccgatcacg ccgacgtcat cgtcgaccac gagcaccttg gaatgcaggt
     1381 tcgtcgggcg gtagagatgg atccgtacgc ccgcctccat catctcgtcg aagtacgacc
     1441 tcccggccag cgacacgatc cgcaggtcgg acgttcccgg gacgatcagg cggacgtcca
     1501 ccccccgcaa ggcggcgttg cgaagggccg cgccgatgcc gtcgtccggg acgaagtacg
     1561 gtgtctcgat ccagagccga cgccgggccg ccgcgaacac cgcgaagacc ccttcgtaga
     1621 tggggcgagt attcctgtcc ggccccgagg tggcgatttg gagggcgcac gggggcgggt
     1681 tccccccctc gaaccgggcg ggtggaggga aaagatctcc cggggggagc gtttccccgg
     1741 tggcgaacgc ccaatcgtcg aggaagagcg cctgcaggtc ggggagagcc ggacccgcga
     1801 tcaccacggc gaggtctctc cattgttcct tcgctacttt cggccccatg tacttctttc
     1861 cgatgttcat tcctcccgtg aacgctttcc gtccgtccgc gatcagcagt ttccggtggt
     1921 tccgcaggtg ggcggaccac cggcgatgga gggggaatgc cggcatgaag gccgccacct
     1981 ccccgcccgc gtcccgcagc ggccgcacgg ttcttcgcag cgcccgccac gacccgacgg
     2041 catcgagcag cagccggacg cggatccctg cccgcgcccg cgcggcgagc gcctggatga
     2101 acctccgccc gacggggtcg acgtcgagga tgaaaaactg tgcgtgcaga tggtcccgcg
     2161 cggatccgat cagggagagg acggcctcgt atgcctcgtc cccgccgcga aggaaggtga
     2221 cgcggttccc ttccgtcggt ggcggcgtcc ccgccgcgag gaggacccgg tcgcaccgct
     2281 ccccgacatc ggtggggagc tcctcgggcc gaagtcggtg ggacagggaa gaggcgaccg
     2341 gcgcgacggc ggcgatcttg gcgcggatgt gccggcggac ccggtggacg ccgatcagga
     2401 aatagagggg gatgccgatc agcggcagga gtgcgatgcc gagcatccac gccagggtcg
     2461 ccgaagggtg gcgccgctcg aggatgacgc gggggatcag gaagaacgcg aagaggtacc
     2521 cggcgatgtt cagtgccagg atcacgttct tccagatgcc tggaattgcc gtcaccgtcg
     2581 catcgccccc gccatgcttg aaggagaacc cttcgcccga tataataccg acttccccgc
     2641 gcggaggggt accgaagtgg ccgtaacggg gtcgactcga aatcgacttg gcggtgcgag
     2701 ccgccacgtg ggttcgaatc ccaccccctc cgccactctt acgaactcct ttgatttggg
     2761 tgggattcga acccgcaca
//
LOCUS       DASVJL010000238         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_412061, whole genome shotgun sequence.
ACCESSION   DASVJL010000238 DASVJL010000000
VERSION     DASVJL010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412061"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1211)
                     /gene="rseP"
                     /locus_tag="VF357_04790"
     CDS             complement(147..1211)
                     /gene="rseP"
                     /locus_tag="VF357_04790"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX7523493.1"
                     /translation="MIYFLSFILVLGVLIFVHEFGHFIVARKLGVGVTKFSFGFGPKL
                     AGFRRGETEYLLSAVPLGGYVKLVGESEGEEVPPEQLSRSFQQKPVWVKMAIVAAGPV
                     GNLVFAILVFWVVFLGGVPSLTTRIGDIAPDTPAARAGLLKGDVVLRIDGATVATWED
                     LALKIRAGSSGRPLRITVKRDGSETTIPVAPEMREGRTVFGEKVSEPKIGIVAGQEVV
                     YRRIGPGAAFLRAGQETGKLIYLTVMTVVKLVTRVLPSETLGGPILIAQIAGDQARQG
                     VSAFAYFLGLLSVNLGILNLLPIPILDGGHLLFFSVEGLMRKPIPPQVRTLAHQVGLA
                     LILTLTALVFYNDIARLLSR"
     gene            complement(1208..>1764)
                     /locus_tag="VF357_04795"
     CDS             complement(1208..>1764)
                     /locus_tag="VF357_04795"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF024684.4"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX7523494.1"
                     /translation="VDSATLMNKGLEVIEATWLFGLPPSRIDVVIHPQSVVHSMVEFR
                     DGSVMAQMGVPDMRIPIGYAFSHPGRLPLELSRLRPHRMEGLIFERPDRKRFPALRIA
                     YAAAETGGSAPAVLSGANEEAVLAFLSGQIAFTDIVRIVDRVLSAWSAPFTARSLQEV
                     LAADEQARREARNELSRHRRHLPS"
BASE COUNT          315 a          594 c          601 g          254 t
ORIGIN      
        1 cggcgaagag cgcttccacc gcggagatgt acgacccgga cgcccgcggg cccggaagga
       61 gaaaatcctc ggcccgaacc tcgccgccgg agaccagcgc cacgctcccg ccgggcgtcg
      121 ccgtctcgag cgccaggatc aacggatcaa cgggacagga gccgggcgat gtcgttgtag
      181 aagacgagcg ccgtcagggt gaggatgagc gcgagcccca cctggtgggc cagcgtgcgc
      241 acctgcgggg ggatcggttt gcgcatcaga ccctcgaccg agaagaagag caggtgcccg
      301 ccgtcgagga tcgggatcgg aagcaggttg aggatgccga ggttgacgct cagcaggccg
      361 aggaagtagg cgaacgcgga gactccctgg cgggcctggt cccccgcgat ctgtgcgatg
      421 aggatcggcc cccccagcgt ctcggacggg agaacgcggg tgacgagttt caccaccgtc
      481 atcacggtga ggtagatgag cttcccggtc tcctgccccg cgcggaggaa ggccgctccc
      541 ggcccgatcc tgcggtagac cacttcctgc cccgcgacga tcccgatctt cggttcggag
      601 accttttccc cgaagacggt gcgcccctcc cgcatctcgg gggccacggg aatcgtcgtt
      661 tccgacccgt cgcgtttcac cgtgatgcgg agaggcctcc cggagctccc cgcgcggatc
      721 ttcaacgcga gatcctccca ggtggccacc gtcgcgccgt cgatccggag caccacgtcc
      781 cctttcagga gcccagcgcg ggcggccggg gtgtcgggcg cgatgtcgcc gatgcgcgtg
      841 gtcagggacg gaacgcctcc gaggaagacg acccagaaaa cgaggatggc gaagacgagg
      901 ttccccaccg gtccggccgc cacgatcgcc atcttcaccc agaccggttt ctgctggaag
      961 gagcgcgaaa gctgttcggg cgggacctct tccccctcgc tctccccgac gagcttcacg
     1021 tacccgccga ggggaaccgc ggagaggagg tattcggttt cgccccgccg gaatccggcc
     1081 agcttcggtc cgaacccgaa cgagaacttg gtgactccga ccccgagttt ccgggccacg
     1141 atgaagtggc cgaactcgtg gacgaaaatg agaacgccga ggacgaggat gaacgagagg
     1201 aaatagatca cgatggaagg tgtctcctgt ggcgggatag ttcgttccgc gcctcgcggc
     1261 gcgcctgctc gtcggcggcg agcacttcct gaagcgatcg ggccgtgaac ggcgccgacc
     1321 acgccgacag cacccggtcg acgatgcgga cgatgtcggt gaacgcgatt tgccccgaca
     1381 ggaacgccag gaccgcctcc tcgttggcgc cgctcaagac cgccggcgcc gaacctcccg
     1441 tctccgcggc ggcgtaggcg atccgcagcg ccgggaaccg cttccggtcg ggccgctcga
     1501 agatcaaccc ctccattcga tgcggccgca ggcgggacag ttccagcggg agccgcccgg
     1561 ggtgcgagaa ggcgtagccg atggggatcc gcatgtcggg gacccccatc tgtgccatca
     1621 cgctcccgtc gcggaactcc accatcgagt ggaccaccga ttggggatgt atcacaacgt
     1681 cgatccgcga gggggggagg ccgaacagcc acgtcgcctc gatcacctcg agccccttgt
     1741 tcatgagggt ggcggagtcc acgg
//
LOCUS       DASVJL010000239         4994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_599302, whole genome shotgun sequence.
ACCESSION   DASVJL010000239 DASVJL010000000
VERSION     DASVJL010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599302"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..926)
                     /locus_tag="VF357_04800"
     CDS             complement(3..926)
                     /locus_tag="VF357_04800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7523495.1"
                     /translation="MISVVVPVYNEAKNLLLLMDRLEAALRKTGQSYEIIYVDDGSRD
                     NSLEVLKGFVGRTGVRVLELTRNYGQHSAIISGFSIVRGEIVITIDADLQNPPEEIPA
                     IVRTMEEGDFEVVGTVREMRKDSLFRKTPSRIVNAMTRRITGIRMSDWGCMLRGYRRE
                     VVDRMVESQEYSTFIPALATLYAKRMTEIPVGHAERHAGVSNYNLWKLLNLQFDLLTS
                     FTEFPLRVLLYMGTGMAFLGVSFGVLLVVMRIFYGAAWALNGIFTLFAVLFFFVGALF
                     FALGIMGQYIGRIYHEVRKRPRFTIRKLHES"
     gene            complement(923..2098)
                     /locus_tag="VF357_04805"
     CDS             complement(923..2098)
                     /locus_tag="VF357_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX7523496.1"
                     /translation="MKIRDDFLPLSRPALGEEEVAAVAESLRSGWITSGPRVAELEGI
                     FRETTGAPHAVAVTSATAGLHIVLSALGIGPGDEVATPSMTFASTVNQIVLRGAVPVF
                     ADVDYGTLQVRPDALAARITPRTRAVIPVHFAGAPADLDPIRAAADRAGIPVIEDAAH
                     AVGTVYKGVPAGGPPRPGTPGNIAIFSFHPIKNITTAEGGMVTCYDDALAVRLRLLRF
                     HGIERDAWKRYGKGGTPHYDIREPGYKYNLTDLHATIGVVQMRKVAALNARRAELAKR
                     YFAGLSGVPGIDLPEAVQYAHAHSWHLFIVKITGMDRDTVIGALAERNVGVGLHFPPC
                     HLLSYVRERFGTREGDLPDTERAGKGIVSLPLFPGMSDADVDYVCEAIREILPGGRP"
     gene            complement(2134..3762)
                     /locus_tag="VF357_04810"
     CDS             complement(2134..3762)
                     /locus_tag="VF357_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX7523497.1"
                     /translation="MSRRAIALFFLSVAVIYYTGLGTLPLLEPDEGRYAEIPREMLAR
                     GDFVTPHLNGVVYLEKPPLFYWGNAISFALFGESEFSARLFTATVSIAGVLLTYWMGA
                     VLGGPRAGLFSAMVLCTSLYHYVIGRINTLDMTLAVTMIVAIFPAFLYLSGKRRSRRY
                     LHLAYAGAALAFLAKGLIGIVFPAAILLLWMLLSKRHREIGKAISLPGLALFLAIALP
                     WVLLVQRANPDFLWFFFVREHFLRFTTKMHQRYQPFWFFFPIVIAGLLPWLVFARRAV
                     RAAWDARETYLLAEDRRFLFCWVLFIFLFFSFSDSKLITYAAPLFPPLAVLFGRALDL
                     WVDTEDGSVRCRTPLALAAILAAGIVLFPPFTRHVIEPSRWVPLCALPAALILAWGVM
                     PLFIRRLSAERVVYLSFLLLALFLTSLNRPAAEYLGNYKSVKMLSSVLSASLREGDVV
                     AQYQTYRQGLPFYTKRRSVLVNEVGELEFGAERAADRKAFFLDDAAFLALWNSPARVF
                     CVGRSDKLPLFREKFPGHRLLYRSDEGILIVNRL"
     gene            3909..4409
                     /locus_tag="VF357_04815"
     CDS             3909..4409
                     /locus_tag="VF357_04815"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEX7523498.1"
                     /translation="MRHLAAIVVALLAAAWVLSPPFPDAAAADIVAAPTGVTIPKGYR
                     NWQVVAPSQRDDKDEIRVILGNNIALKAFRAKRLPFPDGAILAKLAWKRVKSTEFDNT
                     FVPGEPQRIEFMEKNAKKYAATGGWGFGRFVNGAPADEKEHGTCWPCHEANVKGNDFV
                     FTRYAR"
     gene            4432..>4994
                     /locus_tag="VF357_04820"
     CDS             4432..>4994
                     /locus_tag="VF357_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7523499.1"
                     /translation="MGFHVTPLRSGSSGNLTLVEHAGTVLLVDAGLPSQRGLVAALLE
                     ADRAWDDVDALLVSHLHSDHVNGSAVGCCARFEIPIHIHRKNLDGFSKKVLSRSPGSC
                     PVRPFLDGETFSIGAIGIEPFRVPHDARGVTCGFALTSASRDVRVSMATDLGQNENGL
                     FERFLDSDLILIEANYDEEMLNRSPRL"
BASE COUNT          914 a         1590 c         1731 g          759 t
ORIGIN      
        1 gctcagcttt cgtggagctt gcggatcgtg aagcgcgggc gcttgcggac ctcgtggtag
       61 atgcggccga tgtactgccc catgatcccg agggcgaaga agagggctcc cacgaagaag
      121 aacaggacgg cgaacagggt aaagatgccg ttcaacgccc acgcggcccc gtagaagatc
      181 cgcatcacga ccagcagcac gccgaaggag acaccgagaa aggccatccc cgttcccata
      241 tagaggagca cgcgcagcgg gaactccgtg aacgaggtga gcaggtcgaa ctggaggttc
      301 aggagcttcc acaggttgta gttggacacc cccgcgtgcc gttccgcatg tcccacgggg
      361 atctccgtca tccgtttcgc atagagggtc gccagggcgg ggatgaacgt ggagtactcc
      421 tggctctcca ccatccggtc caccacttcg cgtcggtacc cgcgcagcat gcacccccag
      481 tcgctcatgc ggatccccgt gatccggcgg gtcatcgcgt tcacgatccg ggaaggagtc
      541 ttccggaaaa gggagtcttt ccgcatctcc cggaccgtcc ccacgacttc gaagtctcct
      601 tcttccatcg tgcgcacgat ggcggggatc tcctcgggag ggttctgcag gtccgcgtcg
      661 atcgtgatca cgatctcccc ccggacgatg gaaaatcccg agatgatcgc cgagtgctgc
      721 ccgtaattcc gggtaagttc gaggacccgg acgccggtgc gcccgacgaa ccctttcagc
      781 acctcgaggg agttatcccg gctgccgtcg tcgacgtaga tgatctcgta cgactgcccc
      841 gtcttccgca gcgccgcctc gagccggtcc atcaggagga ggaggttctt cgcctcgttg
      901 tagacgggga cgacgacgga gatcatgggc ggcctcccgg aaggatctcc cggatcgcct
      961 cgcagacgta atcgacgtcg gcgtcggaca ttcccgggaa cagggggagc gagacgatcc
     1021 ccttccccgc ccgttcggtg tcgggcaggt cgccctcccg cgttccgaac cgttcccgga
     1081 cgtacgagag gaggtggcat ggggggaagt ggagtcccac gccgacgttg cgctccgcga
     1141 gggctccgat gacggtgtcc cggtccatcc ccgtgatctt cacgatgaac aggtgccacg
     1201 agtgggcgtg cgcgtactgg acggcctccg ggagatcgat ccccgggacg ccggagagtc
     1261 cggcgaagta ccgttttgcg agctcggccc ggcgggcgtt cagcgccgcg accttgcgca
     1321 tctggaccac cccgatcgtt gcgtggagat ccgtcaggtt gtacttgtac cccggttccc
     1381 ggatgtcgta atgcggggtg cccccctttc cgtaccgctt ccacgcgtcc cgctcgatcc
     1441 cgtggaatcg gagcagccgg aggcgcacgg cgagggcgtc gtcgtaacag gtgaccatcc
     1501 ccccctcggc cgtcgtgatg ttcttgatcg ggtggaagga gaagatcgcg atgtttcccg
     1561 gggtgccggg acgcggaggg ccgccggcgg ggacgccctt gtacacggtg cccacggcgt
     1621 gcgcggcatc ctcgatgacg gggatcccgg cgcggtcggc cgccgcgcgg atcgggtcga
     1681 ggtcggccgg cgcgccggcg aaatggacgg ggatgaccgc ccgcgtgcgg ggggtgatcc
     1741 ttgccgcgag cgcgtcgggc cgcacctgga gcgtcccgta gtcgacgtcg gcgaagacgg
     1801 gcaccgcccc gcgaaggacg atctggttga cggtggaggc gaacgtcatc gagggggtgg
     1861 ccacctcgtc ccccgggccg atccccaggg ccgagaggac gatgtggagc ccggcggtgg
     1921 cggaagtgac cgcgacggcg tgcggcgccc cggtggtttc gcggaagatc ccttccagtt
     1981 cggcgactct cggtccgctc gtgatccagc cggagcgcag cgactccgcg accgccgcca
     2041 cctcttcctc gcccagggcg ggacgggaca acggcaggaa atcgtcacgg atcttcaagg
     2101 gcgcgtccgt tcctcacggc gtggtggggt gggtcagagc cggttcacga tgaggatacc
     2161 ctcatcggac cggtagagca accggtggcc ggggaacttc tcccgaaaca gcggaagctt
     2221 gtcgctgcgg cccacgcaga agacgcgggc cggcgaattc cacagggcga ggaacgcggc
     2281 gtcgtcgagg aagaatgctt tccggtccgc cgcccgttcc gccccgaact cgagctcccc
     2341 cacctcgttc acgagaacgg atcgccgctt cgtgtagaag ggaagcccct ggcgatacgt
     2401 ctggtactgc gccacgacgt cgccctcgcg cagggaggcg gagaggacgg aggagagcat
     2461 tttcacggac ttgtagtttc cgagatattc cgccgccggt cggttgaggg aggtgaggaa
     2521 cagggcgagg agcaggaacg acaggtagac gacccgctcc gccgagagcc ggcggatgaa
     2581 cagcggcatc acgccccacg caaggatcag agccgcgggg agggcgcaca gcggaaccca
     2641 ccgggacggt tcgatcacat gccgggtgaa tggcggaaac agcacgatcc cggcggcgag
     2701 gatcgccgcc agggcgaggg gggtccgaca gcggacgctc ccgtcctccg tgtcgaccca
     2761 caggtcgagg gcgcgcccga agaggacggc cagcggaggg aacaacggcg ccgcgtaggt
     2821 gatgagcttc gagtcggaga aggagaagaa gaggaagatg aagaggaccc agcagaagag
     2881 gaaccgccga tcctccgcaa gcaggtaggt ctcccgagca tcccacgcgg cgcgaacggc
     2941 gcggcgagcg aacaccagcc acggaagaag ccccgcgatc acgatgggga agaagaacca
     3001 gaagggctgg taacgttggt gcatcttcgt cgtgaagcgc agaaagtgct cccgtacgaa
     3061 gaagaaccac aggaagtccg ggttcgcccg ctgtacgagg aggacccacg gcagggcgat
     3121 cgcgaggaac agggcgagtc cggggaggga gatcgccttt ccgatctccc ggtgacgctt
     3181 cgacagcagc atccagagca ggaggatcgc cgcggggaag acgatcccga tcaatccctt
     3241 cgcgaggaac gcgagggcgg cgcccgcgta cgcgaggtgc aggtaccttc ggctccgccg
     3301 cttccccgac agatagagga aggcggggaa gatcgcgacg atcatcgtga cggcgagcgt
     3361 catgtcgagg gtgttgatcc gcccgatgac gtagtggtag agggaggtgc aaagcaccat
     3421 ggcggagaag aggccggccc ggggaccccc gagaaccgcc cccatccagt acgtgaggag
     3481 cacccccgcg atcgaaacgg tggcggtgaa gagccgcgcc gagaactcgc tctctccgaa
     3541 gagggcgaag gaaatcgcgt tcccccagta gaagagcggt ggtttctcga ggtacaccac
     3601 gccgttcagg tgcggggtga cgaagtcgcc ccgggcgagc atctccctcg ggatctccgc
     3661 gtaccgcccc tcgtcgggct cgaggagggg gagggttccc agcccggtgt agtagatgac
     3721 cgcgacggag aggaagaaaa gggcgatggc gcgacggctc aaggggcgtc tccggagtgc
     3781 gctattggcg gcatgcccgg gattatactc catcctccgg cggacgaatc cgccgatccg
     3841 ggggccgcgg ggtgcggtct ggtataatgt ccggcaacgt tcgaacccga tcctatgagg
     3901 agaatgccat gcgtcacctc gccgctatcg tcgttgccct gctcgccgcc gcgtgggttc
     3961 tgtccccccc cttccccgac gccgccgccg cggatatcgt tgccgccccg accggggtga
     4021 cgatcccgaa ggggtaccgc aactggcagg tcgtcgcccc gtcccagcgc gacgacaagg
     4081 acgagatccg ggtgatcctc ggcaacaaca tcgcgctgaa ggcctttcgg gcgaagcggc
     4141 tccccttccc ggacggcgcg atcctggcga agctggcgtg gaaacgggtg aagtcgaccg
     4201 agttcgacaa caccttcgtc cccggcgagc cgcagcggat cgagttcatg gagaagaacg
     4261 cgaagaagta cgccgccacc ggcgggtggg ggttcggccg attcgtcaac ggtgcgccgg
     4321 cggacgagaa ggagcacggc acctgctggc cctgccacga ggcgaacgtc aaggggaacg
     4381 atttcgtctt cacgaggtat gcccggtaaa gggagcgagg cggttccggc catgggattc
     4441 cacgtcacgc cgctgcgcag cggaagttcc gggaacctga cgctcgtgga gcacgcgggg
     4501 accgtcctgc tggtcgacgc ggggttgccg tcccaacgag gtctcgttgc cgcgctcttg
     4561 gaggccgacc gcgcctggga cgacgtggac gcgctgctgg tctcccacct ccacagcgac
     4621 catgtgaacg gctccgcggt tggatgctgc gcccggttcg agattccgat ccacatccac
     4681 cggaaaaacc tggacggctt ctcgaagaag gttctctccc ggtcccccgg ttcctgccct
     4741 gtccgcccct tcctggacgg agagacattc tcgatcgggg cgatcgggat cgagccgttc
     4801 cgcgttcccc acgacgcacg gggagtcacc tgcggtttcg ccctcacctc ggcctcccgg
     4861 gacgtccgcg tgtcgatggc gaccgacctc ggccagaacg agaacggcct cttcgagcgg
     4921 ttcctcgaca gcgacctgat cctgatcgag gcgaactacg acgaagagat gctgaaccgg
     4981 agcccccggc tcga
//
LOCUS       DASVJL010000240        13962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_319295, whole genome shotgun sequence.
ACCESSION   DASVJL010000240 DASVJL010000000
VERSION     DASVJL010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 13962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13962
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319295"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VF357_04825"
     CDS             <1..591
                     /locus_tag="VF357_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxxxCH/CxxCH domain-containing protein"
                     /protein_id="HEX7523500.1"
                     /translation="YPNAAGAHAAHIALSSAGTPIACDTCHNGLGTNTLNHYNRANAR
                     PGKNTLRIPPGDVAFLATYSAKTGASSFDNSASLICSNVSCPGGQPTPNWQTGTIDVP
                     NACLSCHISGTTQYNSYFSGEHDRHIGRFGLSATTCKRCHDVAKVNVSGHFQNLATPA
                     FEQTARGTILSSVRYIGNTCNPQAGGLKGCHNSRKW"
     gene            672..1106
                     /locus_tag="VF357_04830"
     CDS             672..1106
                     /locus_tag="VF357_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523501.1"
                     /translation="MRGPHLRSTGKSVIAILCSVCFLTMSLGSVSHVVAADAWETWPK
                     KTAEPVIEQKPATDANGAAKAWEMRPEKTAEPVIEQNPATDANGAAKAGEAAGKEPAK
                     GRSYGTIGWIALGIATVIGIALAAGGGGGGGGGDVVTNPGHH"
     gene            complement(1316..2842)
                     /locus_tag="VF357_04835"
     CDS             complement(1316..2842)
                     /locus_tag="VF357_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX7523502.1"
                     /translation="MVSDPFSGIFSLVDFSYTLISKLAVLSVTFLFFLAVHCLNVFFD
                     KGLSPSKVKATIKIALLGFVILLLDTQVKAAPPYGNILGDALSLIVMVCTANLLTYLV
                     VDIYLYFRMGRQVPSYKRDLTTTLVFIFFAMAALRLIFRIDLTSILTTTTVLTAVVAF
                     AMQTTLANFVSGFYVQNDDNLRSNTWVSLLEHDITGEIVNVGFRYTTIRTLDNHKIML
                     PNNYIMQNVVRVLGTRGGGEKSAVHLKVGLGYDLPPEKAIDMMSRILSQESHIVKVPG
                     PVVIVNNFLDSSIEYDLKYFLDDYSSNDITRGSVLKKIWYAVAREGYSIPYPHREIIS
                     KSPQEPFPEEKRTVLAALQRTEILQSLGEEEIRRLSERVRVRVFGPGEVVVRQNDGGE
                     SLFIVRQGSLDVHIDDAPVGTLREGEIFGEMSLLTGEKRKATVTAASEVRLVEISKED
                     IDPVIRANPQLLEMLSVILARREESNIERRKSAEIQRTGTAKDAFLEKLRTFFGLSAG
                     "
     gene            complement(2937..5087)
                     /locus_tag="VF357_04840"
     CDS             complement(2937..5087)
                     /locus_tag="VF357_04840"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7523503.1"
                     /translation="MNLPASGKKFLEGASVAIASTVLASLLWAAGGLDRWELRTWDWR
                     VSRMSRPAPSTDRVRLVLLDQQSLDWAQKENGLSWPWPREVYGAVIGYLQRAGAKSIA
                     FDVLYTEPSAYGVADDVALGNAIAGSKGFVGALFLGEKTGSVTSWPGAVAAKPPRIRG
                     LDEWLSEGGNRGGVTASRGLFPVPEITGRAAALADVQHNPDSDGVYRRVRLFRVFDGK
                     PVPSLALACFLAAFPDSPIEIRRGSILIAGRTVPIDEEGNAILRFRGPSGTTYRQYSA
                     ASVIQSELRIQAGEDPVIRDPDAFRGRHVIFGFSAPGLYDLRPAPVGGVFPGSEIHAT
                     ALDDLLEGDFMAEVAPRTTVAVTFLLALLAAVSVAFSRNAWRSLLFFAVCLPLPVLLS
                     LFAYSRGYWLPLVVQEAAVSLSLLGALAVNYATEGKQKRYIKNAFRQYLSPDVIEQLI
                     AHPERLKLGGERRTLSIFFSDLQGFTGIAEGLEPEALTALLNEYLSAMTDILQEEGGT
                     VDKYVGDAIIAFWNAPLEQLDHAERAARAAVRCQEKLSELRPSLRKKIGKDLYMRIGL
                     NTGFAVVGNMGSHSRFDYTVLGDAVNLSSRLEGINKQFGTFLLSSEATRKALPETFPA
                     REISRVAVVGRKEPVRVFELFSTKDFAACEREHAVFADGLGAFYEGNFSAALESFLSI
                     EGTDPPAAAYARKCRALMEHPPERWDGVWVMTEK"
     gene            complement(5093..6010)
                     /locus_tag="VF357_04845"
     CDS             complement(5093..6010)
                     /locus_tag="VF357_04845"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7523504.1"
                     /translation="MKTFRGATAIAFLLAGLAGWSGCAAMEQVAQVGTSVAKSQGVVT
                     EEQAKSITRSTTAIAKTQEDFTPEQEYFIGRSVGAVIVNRYKPYPNAPANSYLNLLGQ
                     TLAQASDRPETFGGYCFLILDSDEINAFAAPGGLIFVSRGMLRCCRSEDAVAAVLAHE
                     IAHIQLRHGIQSIEKSRQTQALAILAAEGAKTFGKKEIADLTRTFEGSINDITSTMIN
                     NGYSRSFEKEADASAVRTLSSTGYNPNALVAMLREMEKRLKPGGLDFAKTHPSPGSRI
                     KDIESLVGQAALAEPSARHERFRKAMGTI"
     gene            complement(6007..6507)
                     /locus_tag="VF357_04850"
     CDS             complement(6007..6507)
                     /locus_tag="VF357_04850"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX7523505.1"
                     /translation="MKRHFSALPVFLLVFLMASTTAADAMKMMSVQVKQGEVRVTPYF
                     LGKIVATLSYGDRVEVLGSKESWFHVSFKGKGVTGWMHASALSEKRILLKAGGADAQV
                     GASSGELALAGKGFNADVEAEFKARNRNIDFSWIDRMQAMKVPPERIAAFLQEGGLVP
                     VQGGGK"
     gene            6651..7418
                     /locus_tag="VF357_04855"
     CDS             6651..7418
                     /locus_tag="VF357_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7523506.1"
                     /translation="MIIKVLGAAGSETPGHNCPAFLVDGKFLLDAGTIALALNIREER
                     TLRWIFLSHAHLDHIKGIPFLLDNLVSRSTGNTITVLGGKDVLGDLKKNIFNNRIWPD
                     YTRIPSPGQPSLRYKTIGTTRPCKIDGYTVTMVRVDHTVPAYGYIVEDPGKTAIAYTG
                     DTGPTARFWKRMADHDVKVLITETSFPNRMEKIAMASKHLTPALLGKEIEKMSRPPQR
                     IRIVHLKPQFLQEIESEIRALGHDNIGFLKDGEVIRI"
     gene            complement(7749..8381)
                     /locus_tag="VF357_04860"
     CDS             complement(7749..8381)
                     /locus_tag="VF357_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX7523507.1"
                     /translation="MMKRLAGKTALILLSFLAGMSITAMGLEKPDAFAAGLSVKSLAF
                     GDAESSSEVYIITDWFCPACRIAEPEMLKGARKAMQQAKVIFVDYPIHPETFNYIPYN
                     LSFIVREKEKYLPIREAMATLARKTKEPTPEDVQGAVSPLGVKYVPLNYADVLAGMQY
                     FTTLVKKFKVPGTPSVVVLDSRTGKTKTLYGTSEITSDNIMKTLAEVSGK"
     gene            complement(8943..9251)
                     /locus_tag="VF357_04865"
     CDS             complement(8943..9251)
                     /locus_tag="VF357_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523508.1"
                     /translation="MTRRSWVAATALCLAVVTAAIGPVPQDAIAGILAMSRASGSSLA
                     QEAGPATFRATMDGSQTFHEIVSARYPMWMIMLLAILLLLIWLIYQTWTGWKALIFEK
                     "
     gene            complement(9317..10525)
                     /locus_tag="VF357_04870"
     CDS             complement(9317..10525)
                     /locus_tag="VF357_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEX7523509.1"
                     /translation="MNRWFRVAGGVGLNLCLGAAYVYSVFLTPLHREFGWTRSELSVA
                     FTLSIAFIAAGVLIGGRWQDRRGPAAVSLAGALLFSAGIFLSRYTDSLAWLYGCYGVV
                     VGLGSGIGYTCPLSVGMKWFPERRGTVTGMMVMGYGAGGAVIAPLAGYLIQRFGWRDT
                     FGILGAGFLAVTVAGSMFLRNPPEGWHPPGWSPPAAEKGAVRGGRDFLPREMVSTGAF
                     RRMWVAYAFGTAAGLMVIGHLAAFALGAGFSTRDSALSVGVLSVGNGCGRIASGWMSD
                     HIGRVRTLTLVMSATALLLFLAPGVSTVPLLFAAVFLIGYCYGSQLAVFPSTTADYFG
                     MRNLGNNYGLLLTAWGVAGIIGPMAGGWIFDATHGYAAAFRIAALLAVAAALVVATAK
                     APPPEEERKS"
     gene            complement(10554..11246)
                     /locus_tag="VF357_04875"
     CDS             complement(10554..11246)
                     /locus_tag="VF357_04875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7523510.1"
                     /translation="MKRRLAMAVCAAVAFSGAAFAADAPELKGDKEKISYSIGMDIGG
                     NLKRGSIEVDPDVLAKGLKDSYGGGKTLLTEDQARQAIADFQKTQMAKMAETMEKLAE
                     KNKADGEKFLAENAKKEGVKSLPSGLQYKETTPGKGKSPKASDTVTVQYRGTLIDGTE
                     FDSSYKRGQPATFQVSGVISGWTEALQLMKEGAKWQLFIPSNLAYGEKGAGRDIGPNA
                     TLIFEVELISVK"
     gene            11453..12526
                     /locus_tag="VF357_04880"
     CDS             11453..12526
                     /locus_tag="VF357_04880"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX7523511.1"
                     /translation="MQISVTQAPENRRRQKPKSSEKLGFGKHFSDHMFLMDYSQGTGW
                     RDARIVPYGPLSLAPSAMVLHYAQEIFEGMKAYYARDGAVCLFRPDRNAERMNRSATR
                     MCLPEIPVEDQLAAIRALVRLDREWIPRGEGASLYIRPTMIGTEEGLGVRPSAEVLFF
                     IITGPVGAYYARGFDPVRILVEEQYVRAAKGGVGEAKTGGNYAASLLSAKNAKALGFD
                     QVLWLDAERRCFVEEVGTMNIFFVLRGELVTPPLSGSILPGVTRDTVLTLAQEWKIPV
                     AERPVEIDEVLKGAADGTLSEAFGSGTAAVISPVGSFSCRRQEVAVNGGKAGELAARF
                     FREITGMQYGEIEDRHRWIHRVE"
     gene            complement(12535..12816)
                     /locus_tag="VF357_04885"
     CDS             complement(12535..12816)
                     /locus_tag="VF357_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX7523512.1"
                     /translation="MSIGDGPSATVSVRVVPRSSKEGVAGFEGGVVRIRLNAPPVEGK
                     ANDALVRFLAKAVGVPRSRITLITGERGRSKIVRVAGVTRKALMAALGL"
     gene            complement(12813..13112)
                     /locus_tag="VF357_04890"
     CDS             complement(12813..13112)
                     /locus_tag="VF357_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX7523513.1"
                     /translation="MFVAKNLLEALATIIDYALWAYMWAFILRAVLSWVSPDPYNPIV
                     RALYAVTEPVLSRLRRRLPLFAGSIDFAPIVAVLVIIFLQRFVVRTLFDLALRMP"
     gene            complement(13129..>13962)
                     /gene="proC"
                     /locus_tag="VF357_04895"
     CDS             complement(13129..>13962)
                     /gene="proC"
                     /locus_tag="VF357_04895"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX7523514.1"
                     /translation="SEADRGGADIMALGFLGAGNMGEAMIRGLLSAKLRSPEQVVVYD
                     AAPGRGEELLRRFGIAVARSVEVLLRSCDTVVVAVKPQVLSSVLSGISRDAARGKTFV
                     SIVAGVKIALFEKALGEGAKIVRTMPNTPAQVGMGSTGIYFPPDVDAATRQEVLALFS
                     SFGTVAEMPREELLDAVTALSGSGPAYAFLFLEALADGAVRAGMGRAEASLLAASTLE
                     GAARMVRETGKHPAELKDLVMSPGGTTAAGVAALERGAFRATVMDAVLSAWQRCRELS
                     G"
BASE COUNT         2601 a         4551 c         4410 g         2400 t
ORIGIN      
        1 tacccgaacg cggcgggcgc tcatgccgcg cacatcgccc tgagcagcgc ggggaccccg
       61 atcgcctgcg atacttgcca caacgggctg gggaccaaca ccttgaacca ttacaaccgc
      121 gcgaacgcgc ggccggggaa gaacaccctc cgcattcctc ccggggacgt ggcgtttctg
      181 gcaacgtaca gcgccaagac aggagcgtct tccttcgaca attccgcctc gctcatctgt
      241 tcgaacgtga gctgccctgg aggacaaccc acgccgaact ggcagaccgg gacgatcgac
      301 gtccccaacg cgtgccttag ctgtcacatc tccggcacga cccagtacaa cagctacttc
      361 tcgggagagc acgacaggca catcggcagg ttcgggctca gcgccacgac gtgcaaacgg
      421 tgccacgacg tggcgaaggt gaacgtgtcc ggccacttcc agaacctggc cacgcctgca
      481 ttcgaacaaa cggcccgggg gacgatcctc tccagcgtcc ggtacatcgg caacacctgc
      541 aatccgcagg ccggaggact caagggttgt cacaactcga gaaagtggtg acccggaagt
      601 gttcgcggac agggaccagg catcgacgta acgtttcccc gatgatatga cgtttccaaa
      661 gggggcttga catgcgcgga ccccatctcc ggtctacggg taaatccgtc attgcgatcc
      721 tttgctccgt ctgcttcctc accatgagcc tcgggtccgt ttcccacgtg gtcgctgccg
      781 acgcttggga aacgtggccg aaaaagacgg ccgagccggt tatagaacag aaaccggcca
      841 cggacgcgaa cggggcggcc aaggcctggg aaatgcggcc ggaaaagacg gccgagccgg
      901 ttatagagca gaacccggcc acggacgcga acggggcggc caaggccggg gaagcggcgg
      961 ggaaggaacc ggcgaaggga agatcctacg gaacgattgg ctggatcgcc cttgggatcg
     1021 ccacggtcat cgggatcgcg ctcgccgccg gcggaggcgg aggtggaggc ggaggcgacg
     1081 tggtgacgaa cccaggccac cattgagagc aggcagctcg cccatctgtt gatccgcata
     1141 ccatttccgc ttgcccttgg cgttctcttt gcgggattga tgttcaattc gtactggacg
     1201 tgagggttcg accccctcct ccaacaccaa ggcaatccgc agggttctgg ttaaccgaat
     1261 tgaacgataa agtgttcctg cggacaggcc cacgaggcag gcgcactcgt cctcgtcaac
     1321 cggcggaaag gccgaagaac gtcctcagct tttccaggaa cgcgtccttc gcggtgccgg
     1381 tccgctgaat ctccgccgat ttccttctct cgatgttcga ttcctccctg cgcgccagga
     1441 tgacgctcag catctccagg agttgtggat tcgcgcggat cacgggatcg atgtcctcct
     1501 tcgagatttc caccaggcgt acctcgctcg cggcggtcac cgtggccttc ctcttctccc
     1561 cggtgaggag cgacatctcc ccgaagattt ccccttcccg caacgtcccg accggggcgt
     1621 cgtcgatgtg gacgtccagc gatccctgcc ggacgatgaa gagcgattcc cctccgtcgt
     1681 tctgacgcac caccacttcg ccgggcccga agacccggac ccggacccgt tccgacaacc
     1741 tccggatctc ttcttcgccg agggactgca ggatttccgt ccgctggaga gcggcaagga
     1801 cggtccgttt ctcctccggg aacggttcct gtggggactt ggagatgatc tcccggtggg
     1861 gatacgggat gctgtacccc tccctcgcga cggcgtacca gatctttttc agcacactgc
     1921 cgcgggtgat gtcgttggac gagtagtcgt cgaggaagta cttcaggtcg tattcgatgc
     1981 tgctgtcgag gaaattgttc acgatgacga ccggccccgg caccttcacg atgtgacttt
     2041 cctgcgagag gatccggctc atcatgtcga tggccttttc gggcggcagg tcgtagccga
     2101 gccccacctt cagatgcacc gccgactttt cccccccgcc ccgggtgccc aggacccgga
     2161 cgacgttctg catgatgtag ttgttgggaa gcatgatctt gtggttgtcc aacgtcctga
     2221 tggtggtgta ccggaaacct acgttgacga tttccccggt gatatcgtgc tccagcagcg
     2281 agacccaggt gttgctgcgc agattgtcgt cgttctgaac gtaaaaaccg gagacgaagt
     2341 tggcaagggt cgtctgcatg gcgaacgcca ccacggccgt caacaccgtc gtcgtcgtca
     2401 ggatcgaggt gaggtcgatc cggaagatga gtctcaatgc cgccatggcg aagaagatga
     2461 ataccagggt ggtcgtcagg tcccgtttgt aggaagggac ctgccggccc atccggaagt
     2521 agaggtagat gtcgacgacg agatacgtca ggaggttcgc cgtgcacacc atcacgatga
     2581 ggctcagcgc gtccccgagg atgtttccgt acgggggggc ggccttgacc tgcgtgtcca
     2641 gaagaaggat gacgaagccc agcagtgcga tcttgatggt cgccttgacc ttggacggcg
     2701 acaacccctt gtcgaaaaac acgttcaggc agtgcacggc gaggaagaac aggaaggtga
     2761 cggaaaggac ggcgagcttg ctgatcaggg tgtagctgaa atccaccagg gagaatatcc
     2821 cgctgaacgg atcggagacc atgacccctc ggtcctttcg ggcaggaaaa ggttcacgac
     2881 gatatacggt agccccggaa acgggaaagg tcaatcgccg ggggatctgg cgggagctac
     2941 ttctcggtca tgacccacac gccatcccag cgttccggag gatgctccat cagcgcccgg
     3001 cacttccgcg cgtaggcggc cgcgggagga tcggtcccct cgatcgaaag aaacgattcg
     3061 agggccgcgg aaaaattccc ctcgtaaaac gcgccgaggc cgtcggcgaa gaccgcgtgt
     3121 tccctttcgc aggccgcgaa atctttcgtg gaaaacagct cgaagacccg caccggttcc
     3181 ttccttccca cgacggcgac ccgggagatc tcccgggcgg ggaacgtctc cggcaacgcc
     3241 ttccgcgtgg cttccgacga aaggaggaac gtgccgaact gcttgttgat gccctcgagc
     3301 cgcgaagaca ggttgacggc gtcgccgagg accgtgtagt cgaaacggct gtgggatccc
     3361 atgttgccga cgacggcgaa accggtgttg aggccgatcc gcatgtacag gtccttgccg
     3421 atctttttcc ggaggctggg gcgcagctcc gaaagcttct cctggcagcg caccgcggcc
     3481 cgggcggccc gttcggcgtg gtcgagctgt tcgagagggg cgttccagaa ggcgatgatg
     3541 gcgtcgccga cgtacttgtc gacggtcccg ccctcttcct gaaggatgtc cgtcatcgcc
     3601 gacagatact cgttgagcag cgcggtcagc gcttccggtt cgagcccttc ggcgatgccg
     3661 gtgaatccct ggaggtcgga gaagaagatc gagagggtgc gccgttcgcc gcccagtttc
     3721 agccgctccg gatgcgcgat gagctgctcg atgacgtcgg ggctcaggta ctgccggaag
     3781 gcgttcttga tgtaccgctt ctgtttccct tccgtggcgt aattcacggc cagggcgccg
     3841 agcagcgaca gggaaaccgc cgcctcctgg acgaccagtg gaagccagta tccgcgcgag
     3901 taggcgaaca gggagagcag caccggcagg gggaggcaga ccgcgaagaa gagcaggctc
     3961 ctccaggcgt tccgggaaaa cgcgaccgag acggcggcaa gcagcgccag caggaacgtc
     4021 acggcgacgg tcgtccgcgg cgcgacctcc gccatgaaat ccccttccag caggtcgtcg
     4081 agcgccgtcg cgtggatctc ggaacccggg aatacccctc cgacgggcgc cggccgcagg
     4141 tcgtagagcc ccggggcgct gaacccgaag atgacgtggc ggccccggaa cgcgtccggg
     4201 tcccggatga ccggatcctc cccggcctgg atccgcagct cggactggat caccgatgcg
     4261 gcgctgtact gccggtacgt cgtaccggaa gggccgcgga agcggagaat ggcgtttcct
     4321 tcctcatcga tcgggaccgt cctcccggcg atgaggatcg atccccgccg gatctcgatg
     4381 gggctgtcgg ggaaggcggc caggaagcac gccagcgcga gggaagggac cggtttcccg
     4441 tcgaagaccc ggaacagccg cacccgccgg tagaccccgt cggaatccgg attgtgctgg
     4501 acgtcggcga gggccgcggc ccggccggtg atttccggaa cggggaacag cccgcgggac
     4561 gccgtgacgc ccccgcggtt ccctccctcg gacagccatt cgtccagccc acggatccgc
     4621 ggcggcttcg cggcgacggc ccccggccag gaggtgacgc tgccggtctt ctcgccgagg
     4681 aacagcgccc ccacgaatcc cttgctcccc gcgatggcgt tcccgagggc gacgtcgtcc
     4741 gccacgccgt acgcggacgg ctccgtgtac aggacgtcga aggcgatgct tttcgccccg
     4801 gcgcgctgca gatacccgat gacggcgccg tagacttccc tgggccacgg ccacgacagg
     4861 ccgttctcct tctgggccca atcgaggctt tgctggtcga ggaggacgag tcggacccgg
     4921 tcggtggagg gggccggccg ggacatgcgg ctcacgcgcc agtcccaggt gcggagttcc
     4981 caccggtcca gaccgccggc cgcccacaga agcgacgcca ggacggtgga ggcgatggcc
     5041 accgacgccc cttcgaggaa ctttttcccg gaagcgggca gattcacact cctcagatgg
     5101 ttcccatggc cttgcggaat ctctcatgcc gggcggaggg ctcggcgagg gccgcttggc
     5161 cgacaaggga ttcgatgtct ttgatccggc tgccgggaga aggatgcgtc ttggcgaaat
     5221 cgagcccgcc gggcttcagc cgtttctcca tctcgcggag catcgcgacc agggcgttcg
     5281 ggttgtagcc ggtgctggaa agcgtccgga ccgcggaggc gtcggcctcc ttctcgaagc
     5341 tgcgggaata cccgttgttg atcatggtgg aggtgatgtc gttgatcgag ccctcgaacg
     5401 tcctggtcaa gtcggcgatc tccttcttgc cgaacgtctt ggcgccttcg gccgccagga
     5461 tggccagcgc ctgcgtctgc cggctctttt cgatcgactg gatcccgtgc cggagctgga
     5521 tgtgcgcgat ctcgtgcgcc agcacggcgg cgaccgcatc ttcgctcctg cagcagcgca
     5581 gcatgccgcg ggagacgaag atcaacccgc cgggcgccgc gaaggcgttg atctcgtcgg
     5641 aatcgagaat caggaaacag tagccgccga acgtctccgg ccggtcggac gcctgcgcga
     5701 gggtctgccc gaggaggttc agatagctgt tggccggggc gttcgggtac ggcttgtacc
     5761 ggttgacgat gaccgccccc acggacctgc cgatgaaata ttcctgttcc ggggtaaagt
     5821 cctcctgcgt ttttgcgatc gcggtcgtgc tccgggtgat cgatttcgcc tgctcttccg
     5881 ttacgacccc ttgcgatttg gccacggacg tgccgacctg cgccacctgt tccatggctg
     5941 cgcatcccga ccatccggcc aggccggcga ggaggaacgc gatggcggtc gcgccgcgga
     6001 acgtcttcat tttccacctc cctgcacggg gaccagtccg ccttcctgga ggaacgcggc
     6061 gatcctttcc ggcgggacct tcatcgcctg catccggtcg atccaggaga agtcgatgtt
     6121 cctgttcctg gccttgaatt cggcctccac gtcggcgtta aaccccttgc cggccagcgc
     6181 cagttctccg ctggaggcgc ctacctgcgc atccgctcct cccgccttga ggaggatcct
     6241 cttttcgctc aatgcggagg cgtgcatcca tccggtaacg cctttcccct tgaaggagac
     6301 atggaaccag ctttccttgc ttccgaggac ttcgacgcga tcgccgtaag acaaggtcgc
     6361 gacgattttt ccgaggaagt agggagtcac cctcacctcc ccctgtttca cctggacgct
     6421 catcatcttc atggcgtctg ccgcggtcgt ggaagccatc aggaagacga ggaggaaaac
     6481 cggcaatgcg gaaaaatgcc ttttcatctt tgtccctcca tgaaccgtgg tacgcatgat
     6541 gaataaatta ccggtagcgt cgatcggagt ccagcgtttc ggctcaggcg gccttgaccg
     6601 gccatccccc cgaggctatt aatggagccg gggacaggag gtgctgcccg atgatcatca
     6661 aggttttggg cgccgcgggc tccgaaacgc ccgggcacaa ctgcccggcg ttcctggtcg
     6721 acgggaagtt cctcctggac gcggggacga ttgccctcgc cctgaacatc cgggaggagc
     6781 gcactctgcg ctggatcttc ctctcccacg cccacctcga tcacatcaag gggatcccct
     6841 tcctcctgga caacctggtg agtcgaagca cggggaacac catcaccgtc ctcggcggga
     6901 aagacgttct cggcgacctg aagaaaaaca tcttcaacaa caggatctgg cccgattaca
     6961 ccaggatccc atcgcccggg cagccgtcgc tgaggtacaa gaccatcggc accacccgtc
     7021 cgtgcaagat cgacggctac acggtcacga tggtgagggt cgatcacacc gttccggcgt
     7081 acgggtacat cgtcgaggat ccgggaaaaa cggcgatcgc ctacacaggc gacaccgggc
     7141 cgaccgcccg tttctggaag aggatggcgg accacgatgt gaaggttctg attacggaaa
     7201 cctcgttccc caaccgcatg gaaaaaatcg cgatggcgag caagcacctt acccccgccc
     7261 tcctcgggaa agagatcgag aagatgtccc ggccaccgca acggatccgc atcgtgcatc
     7321 tcaagcccca gttccttcag gagatcgagt cggagatccg ggcgctcgga cacgacaaca
     7381 tcgggttcct gaaggacggg gaagtgatcc ggatctaagt gcttcagttc ataaatacgg
     7441 taacgcaccg agagggtcga tgcgccgcct ccggcaaggc gtcgcgaccg aggcgtactg
     7501 ggcaagtacg gtgagggagc cgccccattc ccgggcaaag tgcactccct tggggtaccc
     7561 cctgacagcc gacaccgtat tgcttgctcg gcatgagggg cacaactccg ccggggaagg
     7621 cagcgatggc catggatggc caagtgccgc ggagggcatg gatgcccggg agcggccggc
     7681 actcgaatgc taccgtagtt atgaattgaa gcactaaggc gcttatcccg ccgccgaagc
     7741 ggcaccattt actttcccga gacctccgcg agggtcttca tgatgttgtc cgaagtgatc
     7801 tcggatgtgc cgtacaaggt ctttgtcttc ccggtacggg aatccagcac aaccacggaa
     7861 ggggtgcccg gcaccttgaa cttcttgacg agggtggtga aatactgcat gccggccaga
     7921 acgtccgcgt aattcaacgg gacatacttc acgccgaggg gagaaaccgc cccctggaca
     7981 tcctccggcg tgggctcctt cgttttccgc gcaagggtcg ccatggcttc ccggatcggg
     8041 agatatttct ccttctcacg gacgatgaag gacaagttgt agggaatgta gttgaaggtt
     8101 tccgggtgga tcgggtaatc cacgaaaatc accttcgcct gctgcatggc ttttcgggcg
     8161 cccttcagca tctccggctc ggcgatgcgg cacgcggggc agaaccagtc ggtaatgatg
     8221 tagacctccg agctgctttc cgcatccccg aaggcgaggg acttcaccga caggccggcc
     8281 gcaaacgcgt cgggtttctc caggcccatc gcggttatgc tcatgccggc gaggaacgag
     8341 agaagaatca acgccgtctt gcccgcgagc cgtttcatca tctgattcct ttctccgcca
     8401 cgaattcgga ttgcgacccc gggcaatcgc tccagggcga tcagcacgca cgctgttacg
     8461 accgagaggg cgactccgac gcacagcggg caccacttcc caataacata cttttgtatc
     8521 cagatgagaa cgaattccga gccgagtgcg ccggagagca gaaaggagat caaaaacccg
     8581 gagaatcgga atcggttcct tgtgagaaaa gcgaggccgc acaatgtgaa atatcctacc
     8641 ccgagagggg aaagcaggag tccgaaaagg cggtacagat gcgtttcctc gcaaccaccg
     8701 gagcagatcc cgagccagga catgacggaa agaatccacg ctgcggcaaa cggcaccccg
     8761 atccaatacc cgcgggtcga tttccccgag cccgtcgaag cggatgcatc catcccgatt
     8821 cctttccctt ctttctcctg ccgcccgaaa gctcaggtcg ggaattattg tatctcccgg
     8881 cagcggaacc cgtgacgggt tttcacctgt cgaagtcaat acggttgttg ttccctacgg
     8941 aactactttt caaaaatcaa tgccttccac ccggtccagg tttggtagat cagccagatg
     9001 agaagcagga gaatggcgag gagcatgatc atccacatcg ggtaccgcgc ggagacgatc
     9061 tcatggaacg tctgcgagcc atccatcgtc gcccggaacg tcgcggggcc cgcctcctgg
     9121 gccagcgacg acccggacgc gcgcgacatc gctaatattc cggcgatcgc gtcctgggga
     9181 acgggaccga tcgcggccgt cacgacggcc aggcagagag cggttgccgc gacccaggat
     9241 ctccgcgtca cccgtcctct tcgtgtccgg aaacctgaat ttttccctgg cacgcggtcg
     9301 tcgaatcttt gtgaactcat gatttccgtt cctcttctgg cgggggagcc ttggccgtcg
     9361 cgaccaccaa ggcggcggcg accgcaagga gggccgcgat ccggaacgca gcggcatagc
     9421 cgtgggtggc gtcgaagatc catccccccg ccatcgggcc gatgatcccg gcgacccccc
     9481 atgccgtcag caggaggccg tagttgttcc cgaggttccg catcccgaaa taatcggccg
     9541 tcgtcgacgg gaagacggcg agctgcgagc cgtagcaata tccgatgagg aagacggcgg
     9601 caaagagcag cggaacggtg gaaaccccgg gggcgaggaa gaggagcagg gcggtcgcac
     9661 tcatcaccag ggtgagcgtc cggacgcgcc cgatgtgatc cgacatccac ccggaggcga
     9721 tccgcccgca cccgttcccc accgaaagca cccccacgga gagggcggaa tcgcgggtgg
     9781 agaatcccgc ccccagcgcg aaggcggcga gatgcccgat caccatcagt cccgccgcgg
     9841 tgccgaaggc gtaggcgacc cacatccggc ggaaggcgcc ggtgcttacc atctcccgcg
     9901 ggaggaaatc ccggccgccc cgcaccgccc ccttctccgc cgcaggagga ctccatcccg
     9961 gagggtgcca cccttccggc gggttccgca ggaacatcga gccggccacc gtcaccgcca
    10021 ggaaccccgc gcctaggatg ccgaaggtat cgcgccaccc gaagcgctgg atcaggtacc
    10081 ccgccagcgg ggcgatcacc gcgccccccg cgccgtatcc catcaccatc attcccgtga
    10141 ccgtcccccg ccgctcgggg aaccacttca ttccgacgga aagggggcac gtgtagccga
    10201 tgccgctgcc cagccccacg acgacgccgt agcagccgta taaccatgcc agcgagtctg
    10261 tgtagcggga gaggaagatc ccggcggaga agagaagcgc gccggcgaga gagacggcgg
    10321 cgggaccccg gcggtcctgc caacgccccc cgatcagcac gcccgcggcg atgaaggcga
    10381 tcgagagggt gaacgcgacg gagagctccg agcgcgtcca gccgaactcg cgatggaggg
    10441 gggtgagaaa cacgctgtag acgtacgcgg cgccgaggca aaggttcaag cccacgccgc
    10501 cggcgacgcg gaaccagcgg ttcactccgc ccccctcccg cgttcgaacc gggtcacttg
    10561 accgagatca gctccacctc gaagatcagc gtggcgttcg ggccgatgtc gcggcccgct
    10621 cccttctcgc cgtacgcaag attcgaaggg atgaaaagct gccacttcgc cccttccttc
    10681 atcaactgga gcgcctcggt ccagccggaa atcaccccgg agacctggaa cgtcgccggc
    10741 tgaccgcgct tataggaact gtcgaactcc gtcccgtcga tcagcgtccc cctgtactgg
    10801 accgtcaccg tgtcggaggc cttcgggctt ttccccttgc cgggcgtggt ttccttgtac
    10861 tggagtccgc tcgggaggga cttcaccccc tccttcttcg cgttctccgc caggaacttc
    10921 tccccgtccg ccttgttctt ctccgcgagt ttttccatcg tctccgccat cttcgccatc
    10981 tgcgtcttct gaaagtccgc aattgcctgg cgcgcctgat cttcggtgag aagggttttc
    11041 ccgccgccgt agctgtcctt caaccctttc gccaacacgt ccgggtccac ttcgatcgat
    11101 ccccgcttca ggtttccccc gatgtccatc ccgatgctgt agctgatctt ctccttgtcg
    11161 cctttcagct ccggggcatc cgcggcgaac gccgcccccg agaacgcaac cgcggcgcat
    11221 accgccatcg caagccgtct cttcatcctt gccgctcctt tccgtcgaaa accggatctg
    11281 tcgtcccgtt ccgtaatttt acacgccggc ccgccgaacc gaaagcggat tcacccgtac
    11341 cggagcgtct ttccggatcg ggaggtttgc tgtataactg attttcgcgc gctttgtctt
    11401 tccacgcgcg ccgaaaggaa cgctgcccag ggagggcggg aagggagagg ggatgcagat
    11461 ttcggtgacg caggcgccgg aaaaccgcag gaggcagaag ccgaagagca gcgagaagct
    11521 cgggttcgga aaacatttct ccgaccacat gttcctgatg gactattcgc agggaacggg
    11581 gtggcgcgac gcccgtatcg tcccgtacgg tccgctgtcg ctcgccccct ccgccatggt
    11641 gctgcattac gcccaggaga tcttcgaggg gatgaaggcg tactacgcgc gggatggcgc
    11701 cgtctgcctc ttccgtccag accggaacgc ggagcgcatg aaccggtcgg cgacacgcat
    11761 gtgtttgccg gagatcccgg tcgaagacca gctcgccgcc atccgggccc tggtgcggct
    11821 cgaccgggaa tggatcccga ggggcgaggg ggcctccctt tacatccgtc cgacgatgat
    11881 cgggaccgaa gaggggctgg gggtccgccc gtcggcggag gtcctcttct tcatcatcac
    11941 cggcccggtg ggggcgtact acgcgcgggg gttcgacccc gtccgcatcc tcgtcgagga
    12001 gcagtacgtg cgcgccgcca aagggggtgt gggagaagcg aaaacgggcg gaaattacgc
    12061 agcgagtctt ctttcggcga agaacgcgaa ggcgctgggt ttcgaccagg tcctatggct
    12121 tgacgccgag cggcggtgct tcgtggaaga ggtggggacg atgaacatct tcttcgtgct
    12181 ccgcggcgaa ctggtcaccc ccccgctctc cggttcgatc ctccccgggg tgacgcggga
    12241 caccgtcctc acgctcgcgc aggagtggaa gatcccggtg gcggagcgcc ccgtggagat
    12301 cgacgaggtc ctgaaggggg ccgcggacgg aacgctctcg gaggcgttcg gctccgggac
    12361 ggcggcggtc atctccccgg tcggctcctt ctcctgtcgc aggcaggaag tcgccgtgaa
    12421 cggggggaag gcgggggaac tcgccgcccg tttcttccgg gagatcaccg gaatgcagta
    12481 cggggagatc gaggaccgcc accggtggat ccaccgggtg gagtgagggg ggatctataa
    12541 gccgagggcg gccatcaagg ccttgcgggt gacgccggca acgcggacga tcttgctccg
    12601 ccccctctcc cccgtgatca gcgtgatgcg gctcctggga acgcccacgg ctttcgcgag
    12661 gaaacggacg agggcgtcgt tggccttccc ctcgaccggg ggggcgttga ggcggatccg
    12721 cacgacgcct ccctcgaacc cggccacccc ctccttcgag gagcgcggga ccacgcggac
    12781 cgacacggtt gcggaggggc cgtccccgat gctcacggca tccggagggc gaggtcgaac
    12841 agcgtccgca cgacgaaccg ctgcaggaag atgatgacga ggaccgcgac gatcggcgcg
    12901 aagtcgatgg agccggcgaa cagcgggagt ctccggcgca ggcgggagag gaccggttcg
    12961 gtcaccgcgt agagggcgcg aacgatcgga ttgtacggat cggggctgac ccacgacagc
    13021 acggcccgaa ggatgaacgc ccacatgtag gcccacagcg catagtcgat aatcgtcgcc
    13081 agcgcttcga gcagattctt cgccacgaac atcccttccc tccccctttc agcccgaaag
    13141 ctcgcggcac cgctgccacg cggacagcac cgcgtccatc accgtcgccc ggaaggcccc
    13201 gcgctccagc gcggccaccc cggcggccgt cgtccccccc ggtgacatca ccaggtcctt
    13261 cagctcggcg gggtgcttcc ctgtctcacg gaccatccgt gccgctccct cgagggtcga
    13321 ggccgcgagg agcgaggcct cggcccgccc catccccgcc cgcaccgcgc cgtccgccag
    13381 cgcttcgagg aagaggaacg cgtaggcggg accggaaccg gagagcgcgg tcaccgcgtc
    13441 gagcagctcc tcgcggggca tctcggccac cgtcccgaag gaggagaaca gggcgaggac
    13501 ctcctgtcgg gtcgcggcgt cgacgtcggg cgggaagtag atcccggtgc ttcccatgcc
    13561 gacctgcgcc ggcgtgttgg gcatcgtccg cacgatcttc gcgccttccc cgagcgcctt
    13621 ctcgaagagg gcgatcttca cgcccgccac gatggagacg aacgtcttcc cccgcgccgc
    13681 gtcccgggag atcccggaca ggacggacga caggacctgc ggtttcacgg cgaccaccac
    13741 cgtgtcgcag gagcgcaaca ggacctcgac ggaacgggcc acggcgatcc cgaaacggcg
    13801 cagcagctcc tccccccgac ccggggccgc gtcgtagacc acgacctgct ccggggagcg
    13861 cagtttcgcg gagagcaagc cgcggatcat cgcctccccc atgtttccag caccgagaaa
    13921 tccgagcgcc atgatatcag ctcctcccct gtctgcttcc ga
//
LOCUS       DASVJL010000241         5020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1631311, whole genome shotgun sequence.
ACCESSION   DASVJL010000241 DASVJL010000000
VERSION     DASVJL010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631311"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VF357_04900"
     CDS             <1..385
                     /locus_tag="VF357_04900"
                     /inference="COORDINATES: protein motif:HMM:NF021915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523515.1"
                     /translation="AVAEVAALAEVRRRFRPEFLNRVDEKIVFRALTSDDVRKVLALR
                     IAELSESLLADHGVALEVDADAAEWLAAEGYRPEYGVRELSRAVDRWIRAPIGAMSAE
                     GELARRAASGTPLTVRKTTEGLRVE"
     gene            542..3118
                     /locus_tag="VF357_04905"
     CDS             542..3118
                     /locus_tag="VF357_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7523516.1"
                     /translation="MKFWQLLVQGQFRHAWEILSRRPDSEHEQALVRTVISFFATIYL
                     SFSVYQDGYLDVQESRLLWMCVLYVLFSVVLFIFITIDPKISPVRRGLGIFGDLSTTS
                     IGIAAAGGAGAPLYVVLLWVIFGNGFRYGTRYLFAASAFGTFAFGAAIHFNAYWQNKL
                     PLGIGLLVGLVVLPIYISSLLKKLTIVGKRAEEANRAKNRFLANMSHEMRTPLNGIIG
                     MLDLLKGTPLSTEQEELTKTIDDSAHTLLFLMQDVLELSKIEAGKVSVEVADFDLYAL
                     VKHTVAIVEPQARSKGLATFLRIPPNVPFLLRGDPLLLRQVLLNLLGNSLKFTEKGEG
                     GVLVTLESETQRRATLRFEVADTGIGIPVEAQRRIFERFTQADESITRRFGGTGLGTT
                     ISKEIVEMMGGTIGVRSEPGRGSTFWFTVEMAKQSRQEDEAVPAAALVDRRALVLSSE
                     PEVAESLRSYLSGWGVHVTTVDRSAQAFARIVSSSNAGTPFDFVLAVKQGLDMDTAGF
                     AHAVRSDSTILESRLILVTQGEDEGKPVAQDGYSAALPAPVDKRILFNALHFSSAGTM
                     ADDPSVGVLAEKYRRKLVGGRKLRILVAEDNRTNQMVIGKILERAGHSFKIVTNGEEV
                     LDAMKEETFDIALLDLHMPVMGGIEAAKLSRFMLPGSSRMPIVALTADATPEARAECK
                     EAGMDACLTKPIDTRMLFDLFETLVPGGGAVRRSPEEGAPEAEETAAAAASETSEKIP
                     CLDPRYLRELSDLGGSSDFVVRLAWTFLKGSKEKVRGLERALADRDVEKVRELAHALK
                     GNSGQIGALSLMGACERFSGIGAGELERNGETYLEEVKEQLSRARTALDEYLGTRNSA
                     VS"
     gene            complement(3141..4079)
                     /locus_tag="VF357_04910"
     CDS             complement(3141..4079)
                     /locus_tag="VF357_04910"
                     /inference="COORDINATES: protein motif:HMM:NF006452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX7523517.1"
                     /translation="MSATLPSLDVGNHSYENMVVRFDIAQGIMWTYFDTRPRPCVTKD
                     LLDEYLECQRLVAQADRTALETGNDCPVRFLVLASKTPGIYSLGGDLHLFKRCIETRD
                     RETLRQYALACIEALHLMANNLFLPLTTISLAQGDALGGGFEAALSCSVIIAERKARF
                     GFPEVLFNLFPGMGAYSFLARRIGMVKAEQIILSGSVFTAEQFHEMGVVDILVEDGGG
                     EGAVHTYVEKHKRRPNAYESVYRARQRYHAVTREELEDIADFWVDAAMRVRAKDLRIM
                     EMLVRSQDRLRSGGPAPVSPLVEGTVVSSSRWSKNA"
     gene            4390..4809
                     /locus_tag="VF357_04915"
     CDS             4390..4809
                     /locus_tag="VF357_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7523518.1"
                     /translation="MKETLKVGLEHVHTYRVPENKTVPHLYPEAKAFQEMPKVFATGY
                     MVGLMEWACIEAMAPHMEPGEGSVGTLVNVTHTAATPPGMTVTVRVRCVGVEGRRSVW
                     DIEAKDDVEVIGRGTHERFAVDFAKFNARVAKKAAGA"
BASE COUNT          939 a         1471 c         1669 g          941 t
ORIGIN      
        1 ggcggtggcg gaagtcgcgg cgcttgcgga ggtccggcgc cggttccgtc cggagttcct
       61 caatcgcgtg gacgagaaga tcgtcttccg ggcgctcact tccgacgacg tccggaaggt
      121 gttggcgctc cggatcgccg agctgtcgga gagcctcctc gccgaccacg gggtggcgct
      181 cgaagtggac gcggacgcgg cggagtggct ggccgcggaa ggataccggc cggagtacgg
      241 agtccgggaa ctttcccggg ccgtggaccg ctggatccgc gcgccgatcg gggcgatgag
      301 cgccgagggg gaactcgcgc gcagggccgc ctcgggcacg cccttgacgg tccgaaagac
      361 gacggaaggc ctccgggtcg agtagcatcc cttttcctga cccggaccgc cgtttccgat
      421 tccatcgatt gccgtatcga tccggcccct tgctcatttt ccggtaaagt ttttcgattc
      481 ccttgccgaa taggatccat agcggcgaaa aacgggttgc gcgcgcgggg agggggggat
      541 cgtgaagttc tggcagttgc tggtacaggg acagtttcgg cacgcatggg agattctctc
      601 ccgtcggccg gactccgagc acgaacaggc gctggtacgg acggttatct ctttctttgc
      661 caccatttat ctgtcctttt ccgtgtacca ggacggatac cttgatgtgc aggagagccg
      721 cctcctctgg atgtgcgtac tctacgttct cttctccgtc gtcctgttca tctttatcac
      781 catcgatccc aagatctccc ccgtccgtcg aggtctgggg atctttggtg atctgagcac
      841 cacgtcgatc ggaattgccg ccgcgggagg cgccggggcg ccactgtatg tcgttctatt
      901 gtgggtgatc ttcggaaacg gattccgata cggcacaaga tatctgtttg cggcctcggc
      961 cttcggcacg tttgccttcg gggcagcgat ccacttcaac gcgtactggc agaacaaact
     1021 tcccttgggg atcggactgc tggtgggcct cgttgtgctt cccatctata tatcgtctct
     1081 cctgaagaag ctcacgatcg tcgggaagcg cgccgaggag gcaaaccggg cgaagaaccg
     1141 gttcctcgcg aacatgagcc acgagatgcg gactcccctc aacgggatca tcgggatgct
     1201 ggacctgttg aaggggacgc cgctttcgac ggaacaggag gaactgacga agacgatcga
     1261 cgactcggcg cacaccctcc tgttcctgat gcaggatgtc ctcgagctct cgaagatcga
     1321 ggccgggaaa gtctccgtcg aggtggcgga cttcgacctg tacgcgttgg tgaagcacac
     1381 ggtcgccatc gtcgagcccc aggcgcggtc caaggggctc gccaccttcc tgcgcattcc
     1441 gcccaacgtg ccgtttctcc tgcgcgggga tccgcttctg ctccggcagg tgctgttgaa
     1501 cctcctcggg aactccttga agttcaccga aaagggcgag gggggggttc tcgtcaccct
     1561 cgaatcggag acgcagaggc gcgcgacgtt gcggttcgag gtggcggaca ccggcatcgg
     1621 gatccccgtc gaggcgcagc ggcgcatctt cgagcggttc acccaggcgg acgagtcgat
     1681 cacccggcgg ttcggcggga cggggctggg aacgacgatt tcgaaggaga tcgttgagat
     1741 gatgggtggg acgatcgggg tccggagcga accgggccgg ggcagcacct tctggttcac
     1801 cgtggagatg gcgaagcaga gcaggcagga ggatgaggcg gtgcccgcgg cggcgctcgt
     1861 ggatcggcgc gccctcgtcc tctcgtcgga acccgaagtg gccgaatccc tccgttcgta
     1921 cctttcgggg tggggagtcc acgtcaccac ggtggaccgg tccgcccagg cgttcgcccg
     1981 gatcgtcagc tcttcgaacg cgggaacccc cttcgatttc gttctggccg tcaagcaggg
     2041 tctggacatg gacacggccg ggttcgccca cgccgtccgt tccgattcca cgatcctcga
     2101 gagccggctc atcctcgtga cccaggggga agacgagggg aagcctgtcg cgcaggacgg
     2161 ctacagcgcc gccctgccgg cgccggtgga caagaggata ctcttcaacg ccctgcattt
     2221 ttcgagcgcc gggaccatgg cggacgatcc ctcggtcggg gtcctcgcgg agaagtaccg
     2281 gcggaagctc gtgggcggtc ggaagctgcg catcctggtg gccgaagaca accggacgaa
     2341 ccagatggtg atcggcaaga tcctcgagcg ggcagggcat agcttcaaga tcgtgacgaa
     2401 cggggaagag gttctcgatg ccatgaagga ggagacgttc gacatcgcgc tccttgatct
     2461 ccacatgccg gtgatggggg gcatcgaggc ggcgaagctg tcccggttca tgttgccggg
     2521 ttcttcgcgg atgccgatcg tggcgctcac ggcggacgcg acgccggagg cgcgggcgga
     2581 gtgcaaggag gcggggatgg acgcgtgcct caccaagccc atcgacacgc ggatgctgtt
     2641 cgacctcttc gaaacgctgg ttcccggtgg gggcgccgtg cggaggagcc cggaggaggg
     2701 cgccccggaa gcggaggaga cggcggcggc agcggcatcc gagacgtcgg agaaaatccc
     2761 atgcctcgac ccacggtatc tccgcgagtt atcggacctc ggcggaagca gcgacttcgt
     2821 ggtccgcctg gcgtggacct ttctcaaggg atcgaaggag aaggtgcgag ggctcgagcg
     2881 cgccttggcc gatcgtgacg tcgaaaaggt ccgcgagctg gcgcacgcgt tgaaggggaa
     2941 ctcggggcag atcggcgcgc tctcgctgat gggggcatgc gagcgatttt ccggcatcgg
     3001 cgcgggggag ctggaacgaa acggggaaac ctacctcgaa gaggtgaagg aacaactctc
     3061 ccgtgcccgg acggcgctgg acgaatacct gggaacaagg aactccgcgg tttcctgaga
     3121 attccgtccg agggaacggg tcaggcgttc tttgaccatc ggcttgatga gacaacggtc
     3181 ccttcaacca agggactgac cggggccggc ccgccggagc ggagacggtc ttgggacctc
     3241 acgagcatct ccatgatccg gagatccttc gctctgacgc gcatggcggc gtcgacccag
     3301 aagtcggcga tatcctcgag ttcctcgcgg gttaccgcgt ggtaacgttg ccgcgccctg
     3361 tagacggatt cataagcgtt tgggcggcgc ttgtgtttct ccacataagt gtggacggcg
     3421 ccctccccac ccccatcctc gacgagaata tcgacgaccc ccatctcgtg gaattgctcg
     3481 gcggtaaaaa cggagccgct caggatgatt tgctcggctt tgaccatccc gatgcgccgg
     3541 gcgagaaaac tgtaggcccc catcccgggg aagaggttga acagaacctc cgggaagccg
     3601 aaccgggcct ttctctcggc gatgatcacg gaacaggaca gcgctgcctc gaagcctccg
     3661 cccaaggcgt ccccctgggc caatgagatc gtcgtcaacg gaaggaacag attgttcgcc
     3721 attagatgga gcgcctcgat acacgccaat gcgtactgcc tcagggtttc ccggtcccgg
     3781 gtttcgatgc accgcttgaa caggtggagg tcccccccga gactgtagat gcccggtgtc
     3841 ttcgacgcaa ggacgaggaa tcggaccggg cagtcgttcc cggtctcaag agcggtcctg
     3901 tcggcctggg ccacgaggcg ctggcattcg aggtactcgt cgagcagatc cttggtgacg
     3961 cagggcctcg gccgggtgtc aaaatacgtc cacatgatcc cctgcgcgat atcgaaccgg
     4021 acaaccatat tctcgtagga atggttcccc acatcaagac tgggtagtgt ggcgctcatc
     4081 ggtgacactc cctgtcaaac aaaagttgta ggggtcgttg tacagcagaa attaaggtta
     4141 tgcaatataa atgatatata accctttaaa tcgcgctata ttttttttat ttctctctat
     4201 atacgcttga gagtagcatg cggttctgga aaagtaaata aaacaaattc gatttgccta
     4261 taagtggaaa tgattttcct ttctccgttc ttctctctcc gccccattgt aacctggggg
     4321 cggaaaggct atctttcccg gatacgaaat cgccgctgtc cccgcgatac accgccgaag
     4381 gagggcctca tgaaagagac actgaaggtc gggctggagc atgtgcacac gtatcgtgtt
     4441 ccggagaaca agacggttcc ccacctctac cccgaggcga aggcgttcca ggagatgccg
     4501 aaggtgttcg ctaccggcta tatggtcggg ctcatggagt gggcatgcat cgaggcgatg
     4561 gcgccgcaca tggagccggg ggaggggagc gtcgggacgc tcgtcaacgt gacgcacacc
     4621 gcggcgaccc cgcccgggat gaccgtcacg gtccgcgtcc ggtgcgtcgg ggtggagggg
     4681 cgccggtcgg tgtgggacat cgaggcgaag gacgacgtcg aggtcatcgg caggggaacc
     4741 cacgagcggt tcgccgtcga tttcgcgaag ttcaacgccc gcgtcgcgaa gaaagccgcg
     4801 ggggcgtaaa tcccgggccg gagcgcggga tcgcccccct ccgggagatc cttcctcccc
     4861 ccgggaaacc tcccggggga gccgcaactt tatttaccga tcggtaaatt ttccgttgac
     4921 acggttgcgc ccgcccgtat acttaccggt aggtaaatat tggaccgcaa ggcggcggga
     4981 gggcggtatg gcgaaaaggg agcggaaaga ggccgaggcg
//
LOCUS       DASVJL010000242         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_319345, whole genome shotgun sequence.
ACCESSION   DASVJL010000242 DASVJL010000000
VERSION     DASVJL010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319345"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VF357_04920"
     CDS             <1..902
                     /locus_tag="VF357_04920"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7523519.1"
                     /translation="PALLVEGYPERFSLGLLTSSGSLGLLFPPCLPVILYAVVAGIAV
                     DKMFLGGFLPGILLTLLMAGWAVRGGVRAGARRTPFSWRELGAATWDAKWDLLLPVIV
                     LVGIFGGVATAVEAAAMTVLYAIGTEVFVHRDAGFRRLLSEAGTETSRLVGGFLLLLA
                     AATGLTGYMVDAGVPQILFEWVRSTIDSRFTFLLVLNVFLLLVGAFMHIFSAIVIVVP
                     IIAPLAASYGVHPVHLGIIFLANLELGFLMPPVGMNLFMSAYRFEKPFGEVCRAVLPF
                     LLLLLAGVLAITYVPWLSTALLP"
     gene            complement(880..2412)
                     /locus_tag="VF357_04925"
     CDS             complement(880..2412)
                     /locus_tag="VF357_04925"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX7523520.1"
                     /translation="MTLAPAPLPIAKGKKEIALLPGMANRHGLIAGATGTGKTVTLRV
                     LAERFSAIGVPVFLADVKGDLSGIPRPGGNNPKVAERVTQLKLDGFEYHGYPVTFWDL
                     FGEQGHPVRATVSEMGPLLFGRILNLNDIQCGVLTLVFKIADDSGMLLLDLKDLRSMA
                     QFAGDNAEQFRTSYGNIAAASVGAIQRGLLMLEEQGGDRFFGEPALDLQDLLQTDPAG
                     RGMINILAASRLMTSPRLYSTFLLFLLSEMFEQLPEVGDPQKPGLVFFFDEAHLLFTD
                     APKPLLEKIEQVVRLIRSKGVGVYFITQNPMDIPERVLGQLGNRVQHALRAFSPNEQK
                     AVKAAAETFRANPKLDVVKAITELGVGEALVSVLDAKGTPSVVERAFILPPRSRLAPL
                     SPAERAQVVRDSVLHGHYENAVDRESAYEKLKEKASQRQAEVSAGGGSGRSLEMRAGP
                     RVAVPGRCADRGAGQERGPRHRQPDRAPDHPGGVGLHPRRQALTGAQRKIQRQAKAAG
                     RC"
     gene            2551..3111
                     /locus_tag="VF357_04930"
     CDS             2551..3111
                     /locus_tag="VF357_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523521.1"
                     /translation="MLKKEDCVLYSGGAVGAEAEFGAQAERYGIEEINFTYESRDIAR
                     SRGVRVLSREELARGDVSLTYVSRLLNRSYTDAPTIRKVLQTIWYQVNSGHEVYVIGA
                     VLPDGTVKGGTGWGAEFAKLCNKPIFVFDQGKNGWVTWKDDRWVEPAGGQAIRIGNPC
                     FAGTGTRFLEENGRKAIQELFARSFA"
     gene            3124..>3455
                     /locus_tag="VF357_04935"
     CDS             3124..>3455
                     /locus_tag="VF357_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7523522.1"
                     /translation="MKQATADIRNVAIIAHVDHGKTTLVDAMLRQSGIFSAHAAVVDR
                     VMDSLDLEREKGITIMAKNTAVIYRGVKINIVDTPGHADFGGEVERTLKMVDAVLLLV
                     DASEGPLP"
BASE COUNT          528 a         1158 c         1136 g          633 t
ORIGIN      
        1 tgcccgcgct cctcgtggag gggtacccgg agaggttctc cctcggcctt ctcacctcgt
       61 ccgggtccct cgggctcctg ttccccccct gcctgccggt gatcctgtac gcggtcgtgg
      121 ccgggatcgc ggtggacaag atgttcctcg gcgggtttct ccccgggatc ctccttacat
      181 tgctcatggc gggctgggcc gtccggggag gggtgcgcgc gggggcgcgg cggacaccgt
      241 tctcctggag ggaactgggc gcggcgacgt gggatgcgaa gtgggacctt ctcctccccg
      301 tgatcgtgct ggtgggcatt ttcggggggg tcgcgaccgc cgtcgaggcg gcggcgatga
      361 cggtcctcta cgccatcggc accgaggtgt tcgtgcaccg cgacgcgggg ttccggcggc
      421 ttctgtccga agcggggacc gaaacttccc ggctcgtcgg ggggttcctc ctcctcctcg
      481 cggcggccac ggggcttacg ggttacatgg tcgacgccgg ggtcccccag atcctcttcg
      541 agtgggtccg ctcgacgatc gactcccggt tcaccttcct cctcgtcctg aacgtgttcc
      601 tgctcctcgt cggcgccttc atgcacatct tctcggcgat cgtgatcgtc gttccgatca
      661 tcgctccgct cgccgcgtcg tacggcgtgc accccgtgca cctcgggatc atcttcctcg
      721 cgaacctcga gctcgggttc ctgatgcccc cggtagggat gaacctcttc atgtccgcct
      781 atcgcttcga aaagccgttc ggcgaggtgt gccgcgcggt cctccccttc ctgctcctgc
      841 tgctggcggg cgtcctcgcc atcacctacg tcccctggct cagcaccgcc ctgctgcctt
      901 agcctgcctc tgaatcttcc gctgtgcgcc ggtcagcgct tgccgccgag gatggagccc
      961 aacacccccc ggatgatctg gcgcccgatc tggctgccga tggcgtgggc cgcgctcttg
     1021 gccagcgccc cgatcagcac accggcctgg gaccgcgact cgggggcccg cccgcatttc
     1081 gaggctccgc ccggaacccc cccccgcgct tacctccgcc tgccgctgcg acgccttctc
     1141 cttcagcttc tcgtaggctg actcccgatc caccgcgttc tcgtaatgtc cgtggaggac
     1201 cgaatcgcgg acgacctgcg cccgttccgc gggcgagagg ggagcaagcc ggctccgggg
     1261 cggcaggatg aaggcgcgct ccaccacgga cggcgtccct ttcgcgtcca ggacggagac
     1321 gagcgcctcc ccgacgccga gttcggtgat cgccttcacg acatcgagct tcggattcgc
     1381 ccggaacgtt tcggcggcgg ccttgaccgc cttctgctcg ttgggggaaa acgcccgcaa
     1441 ggcgtgctgc acccggtttc ccagctggcc gaggacccgt tcggggatgt ccatggggtt
     1501 ctgggtgatg aaatacaccc ccacgccttt cgagcggatg agccgcacca cctgctcgat
     1561 cttttcgagg agcggcttcg gcgcgtccgt gaagagcagg tgtgcctcgt cgaagaagaa
     1621 gacgaggccg ggtttttgcg ggtctcccac ctcggggagt tgctcgaaca tctcggagag
     1681 aagaaataac aggaaggtgg agtagagcct gggagaggtc atgagccggg atgcggcgag
     1741 gatgttgatc atcccgcggc cggccggatc ggtctgaagg aggtcctgca ggtcaagtgc
     1801 gggctcgccg aagaaccggt cccctccctg ttcctccagc atgaggagcc cccgctggat
     1861 cgcaccgacg ctcgcggcgg caatgttgcc gtacgaggtc cggaactgct cggcgttgtc
     1921 cccggcgaac tgcgccatgg accgcaggtc cttgaggtcc agcagcagca tgccgctgtc
     1981 gtccgcgatc ttgaagacga gggtcagcac gccgcactgg atgtcgttca ggttgaggat
     2041 ccgcccaaag aggagaggtc ccatctccga gacggtggcg cgcacggggt gcccctgctc
     2101 cccgaagagg tcccagaagg tgacgggata tccgtggtat tcgaacccgt ccagtttgag
     2161 ttgagtcacc cgttcggcga ctttcggatt gttgcctccc ggccgcggaa tcccggacag
     2221 gtcccccttc acgtccgcca ggaagaccgg aacgccgatt gcgctgaacc gctccgccag
     2281 cacacgtagc gtgaccgtct tgccggtgcc cgtggcgccg gcgatcaggc cgtggcggtt
     2341 tgccatcccg ggaagcagcg cgatctcctt ctttcccttg gcgatcggga gcggcgccgg
     2401 tgcgagcgtc atcggtcaac ctcctggtgt gttatggagc gaacgacgga tcctgcatat
     2461 tacatcacag tgggcgttcg aatttccgcg agcccgccgc cttcgtggta tagtggtgcc
     2521 gattcaccca cccaccccaa ggagtgtgcg atgctcaaga aggaggattg cgtcctctac
     2581 agcggcggtg ccgtcggcgc cgaggcggag ttcggcgcgc aggcggaacg gtacgggatc
     2641 gaggagatca atttcacgta tgaaagccgg gacatcgccc gctcccgcgg cgtccgcgtc
     2701 ctctcccggg aggagttggc ccggggggac gtgagcctca cctacgtgtc gcgcctcctg
     2761 aaccggagct acaccgacgc ccccacgatc cggaaggtcc tgcagacgat ctggtaccag
     2821 gtcaattccg gccacgaggt ctacgtgatc ggggcggtcc tccccgacgg gacggtcaag
     2881 ggcggcaccg gttggggggc cgagttcgcg aagctgtgca acaagccgat cttcgtcttc
     2941 gaccagggga agaacgggtg ggtcacctgg aaggacgacc ggtgggtcga accggcgggc
     3001 gggcaggcga tccgcatcgg caatccctgt tttgccggga cgggaacccg cttcctcgaa
     3061 gagaacggcc gaaaagcgat ccaggagcta tttgcgcgct ccttcgcgta aagccccctc
     3121 ctcatgaagc aggccaccgc cgacatccgg aacgtcgcga tcatcgccca cgtcgaccac
     3181 gggaagacca ccctggtcga cgcgatgctg cggcagagcg gcatcttcag cgcccacgcg
     3241 gccgtcgtcg accgggtgat ggactccctc gatctcgagc gggagaaggg gatcacgatc
     3301 atggcgaaga acaccgccgt gatctaccgg ggcgtcaaga tcaacatcgt ggataccccc
     3361 ggtcacgccg acttcggcgg cgaggtggag cgaacgctga aaatggtcga cgcggttctg
     3421 ctgctcgtgg acgcctccga gggccctctt ccgca
//
LOCUS       DASVJL010000243         9256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_507906, whole genome shotgun sequence.
ACCESSION   DASVJL010000243 DASVJL010000000
VERSION     DASVJL010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507906"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VF357_04940"
     CDS             <1..479
                     /locus_tag="VF357_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523523.1"
                     /translation="VPVSPAPQVPPSGNPRTGEALFLGRIAFRNGGAPCFACHNVSGA
                     GPLGGGTLGPDLTGAYTNFGEVGISSILATLPFPTMKPIFDSRPLTPEEREDLKAFFR
                     QSAGHPPGTPRAAIGALAAGGWIVLLLLTQATWRGRLRGVRRTLPTKAAGTGGGGE"
     gene            476..4093
                     /locus_tag="VF357_04945"
     CDS             476..4093
                     /locus_tag="VF357_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEX7523524.1"
                     /translation="MSWIRDLVKPEARLWEEFYRNRWQHDRVVRSTHGVNCTGGCSWK
                     VYVKDGIITWEMQETDYPLLEAGLPGYEPRGCQRGISFSWYIYSPLRIKYPYLRGTLA
                     ELWRAAKDAHSDPVAAWGSIVEDEEKRRSYQQARGKGGFRRIPWEEALEIVAASSLYT
                     AKRYGPDRVVGFSPIPAMSMCSYASGTRFLQLFGGVVLSFYDWYADFPPASPEVWGEK
                     TDVAESADWYNSKLIAIVGTNLSMTRTPDVHFAAEARNNGSRLVVLSPDFSQVSKYAD
                     WWLPVNAGMDGAFWMAVDHVLLKEFYADRQVPYFLDYLKRYSDAPFLVALEESAEGCR
                     PGRFLRADALPRYRNAENAGWKFLVFDETSGAPKMPLGSIGFRWQEEKGKWNLEMKDG
                     LDGAEIRPLLTFLESREAVLPVTFSGFEDGKTFRRGVPVRYVETERGNVPVTTVFDLL
                     MARFGVGRGLEGEFPADYNDGEALYTPAWQEPYTGVGRETVIRFAREWASTAEKTGGK
                     CTILIGSGANHWYHANLNYRAGITALVLCGCVGVNGGGLNHYTGQEKVTPEASWSTLA
                     FAKDWGKPSRLQNAPSFHYVHSDQWRYDLGVADLPPGESPGVPGRKHPVEYLADSVRM
                     GWLPFTPQFNRNPAELVREAEAAGAKSDAEIVGWVVEQLRSGNLRFSVEDPDAPENWP
                     RIWLIWRGNALLASAKGHEYFLKHYLGTHHSSVADETASGTAGDVNRREPAPRGKLDL
                     VVDVNFRMDTSALYSDVILPAATWYEKDDLNTTDLHSFIHPLSAAVPPCWESRTDWDI
                     FKTIAKKTSELARLHGPEPFREIVASPLLHDSPDEIAQPEVKRWYEGECEPVPGKTMP
                     HLAVVERDYANLYDRFVSLGDSLRENGMEEKGIHWPVADLYDELLESAPVREWNGRNY
                     PSLEEVVDAANVILHLAPETNGEAAYTAFRAQERIVGLPLADLAEKQRGTRYRFADLV
                     AQPRRVLTSPCWSGITNEGRPYSAYCINVERRVPWRTLTGRQHFYLDHEWYLAFGENL
                     PAFKSKVDLESSGNLAKSRAEGKALVLHYLTPHGKWHMHTTFSDNHRMLTLSRGMEPF
                     WMNDRDAAEIGVADNDWVEAYNDNGVVVTRAVVSARVPRGTGIYYHAPERTISVPRSP
                     LRNNRRAGGHNSLTQTRLKPVLMTGGYAQLCYAFNAWGPPGPDRDTYVYVHKLEGKPR
                     W"
     gene            4113..5600
                     /gene="narH"
                     /locus_tag="VF357_04950"
     CDS             4113..5600
                     /gene="narH"
                     /locus_tag="VF357_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEX7523525.1"
                     /translation="MNIRAQVSMVFHLDKCIGCHTCSIACKNIWTDRRGTEYMWWNNV
                     ETKPGTGYPTLWEDQEKYHGGWVKEAGTVRLKLHSRKSGLANLFHNPFLPTMDDYYEP
                     WTYRYEDLLNAPKGEDQPTARPVSMITGKPIDIEAGPNWDDDLGGSQIYASNDPNLAA
                     ATPEERRQLFALERIVFFYLPRICNHCLNPGCVAACPAGAVYKRGEDGIVLVSQEKCR
                     AWRMCISGCPYKKVYYNWETGKSEKCILCYPRQETGQAPACFHSCVGRIRYLGMLLYD
                     ADRIERTAGRPDREMAEAQREMILDPSDPEILRKAREDGVHEAILEAAVKSPVYRYLK
                     EWKLALPLHAGFGTLPMLFYVPPLLPEAASVREGRYDPGNDLFGSIEKARLPVHYLAS
                     LFSGGDEEAIKAIYRKLIAVRIYRRSKTVGDIPEEELERALTGAGIGAGEAEAIYRMT
                     SLAPFEERHVIPPFLREMAIEQTTDPGVHKTETGVGTLRPPKRGL"
     gene            5601..6161
                     /gene="narJ"
                     /locus_tag="VF357_04955"
     CDS             5601..6161
                     /gene="narJ"
                     /locus_tag="VF357_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEX7523526.1"
                     /translation="MEDAFEARYRLFADLLSCPTPSLPARAREGASLLASVDGDAADL
                     VDRFRDFLEETPPGRLEEIHTGVFDLQPVCCPYVGYHLFGEHYRRGIFMARLREHYRD
                     HSFPEDKELPDHIVVLLRYLAGRHDEETDRDLVTECLLPAAEKMVKGFTDGTNPYRGI
                     LEALVRVLRREAASSANADPRKEVDR"
     gene            6158..6832
                     /gene="narI"
                     /locus_tag="VF357_04960"
     CDS             6158..6832
                     /gene="narI"
                     /locus_tag="VF357_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEX7523527.1"
                     /translation="MTDLFLFVVFPYVALALAVAMGVHRYVADRFSFSSLSSQFLENR
                     ALFWGSVPWHYGIVSILVAHLLAALFPGSWGSLLGPPGRLYFLEVTGMALGLFTLVGL
                     LLLIARRAGNARIRSVTTVMDGILLADLALQVATGLYIAYSYRWGSLWYLHTAVPWLS
                     SLARLQPVTAGIAPLPPVVRFHLFNAFIVVGLFPFTRLVHIFTVPIEYLWRPWQVVLW
                     NRRPPP"
     gene            6829..7752
                     /locus_tag="VF357_04965"
     CDS             6829..7752
                     /locus_tag="VF357_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523528.1"
                     /translation="MNRFAFSIDPIPPFRLDLTAWTLRRRPGNIVDRWDGKTYRRALV
                     VGSHPLEVEVKQTGPPEAPRLKVRATSAVPFPGEGSAVRTIVERILGARIDLGGFYRF
                     AAGDPALGPVVTRFRGAKPPRFPTLFETLTNAIACQQLSLTVGILLLNRMTEACGAAI
                     PGSDPAVHAFPSPEDVAALAPETLRSLGFNRQKARALLELAGVVAGKRIDLDGIASLE
                     DEAAVDRLQELRGVGRWTAEYVLLRGLGRIHVFPGDDVGARNHLLRWLGKRKTLGYEA
                     VRSALSRWKPYAGLVYFHLLLKGLSEAGRFP"
     gene            8390..>9256
                     /gene="sufB"
                     /locus_tag="VF357_04970"
     CDS             8390..>9256
                     /gene="sufB"
                     /locus_tag="VF357_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX7523529.1"
                     /translation="MSDSTRDVKERLNREYRWGFVTPMEEDSVPKGLNEGIIRTISAR
                     KNEPEFLLEQRLKAYRHWLTMTEPKWSNVHYPPIDYQDIVFYSAPRTNKAKPTSLDEV
                     DPVLLNTYEKLGIPLRERELLAGVAVDAIFDSVSVATTHQKELAEQGIIFCSFSEAVR
                     SHPGLVKKYLGSVVPHTDNFFASLNSAVFSDGSFVYIPPGVRCPMELSTYFRINAADT
                     GQFERTLIVADAGAYVSYLEGCTAPRRDENQLHAAVVELIALENAEIKYSTVQNWYPG
                     DKEGKGGIYNFVT"
BASE COUNT         1695 a         2987 c         3014 g         1560 t
ORIGIN      
        1 cggtcccggt ttcgcccgct ccgcaggttc ctccatcggg gaaccctcga accggcgaag
       61 cgctcttcct ggggaggatc gccttccgga acgggggggc gccgtgcttt gcgtgtcaca
      121 acgtttccgg ggcaggcccc ctgggcggcg gcaccctcgg gccggacctc accggcgcct
      181 acacgaattt cggcgaggtc gggatctcct ccattttagc gaccctcccg ttccccacca
      241 tgaagccgat cttcgattcc cgcccgctga ccccagaaga gcgagaagat ctgaaggcgt
      301 ttttccgtca atcggccggc caccctccgg gaaccccgcg ggcggcgatc ggtgcgctgg
      361 cggcgggagg atggatcgta ctgttgctgc tcacgcaggc gacctggcgc ggccgacttc
      421 gaggggtccg caggacactg ccgacaaaag ccgcggggac ggggggaggc ggggaatgag
      481 ctggatccgg gacctggtca agccggaagc gaggctctgg gaggagttct accggaaccg
      541 gtggcagcac gatcgcgtgg tgcgcagcac ccacggagtc aactgcaccg gcggttgttc
      601 gtggaaggtg tacgtgaagg acggcatcat cacctgggag atgcaagaga ccgactaccc
      661 gcttctcgag gccggccttc ccggctacga gccccgcggc tgtcagcgtg gcatctcctt
      721 ctcctggtac atctacagcc cgctccggat caagtatccc tatctccggg gaacgctcgc
      781 ggagctttgg cgcgcggcga aagacgcgca ttccgatccg gtagccgcat ggggatccat
      841 cgtggaggac gaggagaaac ggcgcagcta ccagcaggca cgcgggaaag gcggtttccg
      901 ccggattcct tgggaggagg ccctcgagat cgtcgccgcc tcctccctct acacggcgaa
      961 aaggtacggg ccggaccggg tggtcgggtt ctcccctatc ccggccatgt cgatgtgcag
     1021 ctacgcttcc ggcacgaggt tcctgcaact gttcggcggc gtcgtcctga gcttctacga
     1081 ctggtacgcc gacttccccc cggcttcccc ggaagtctgg ggggagaaga ccgacgtcgc
     1141 cgaaagcgcc gattggtaca acagcaaact gatcgcgatc gtggggacca acctcagcat
     1201 gacccgcacc ccggacgtcc acttcgccgc ggaagcacgc aacaacggga gccggctcgt
     1261 ggtcctctcc ccggacttca gccaggtctc gaaatacgcg gactggtggc tccccgtgaa
     1321 cgccgggatg gacggcgcct tctggatggc ggtcgaccat gtccttttga aggaattcta
     1381 cgcggaccgg caagtcccgt attttctcga ttacctgaag cgctattcgg acgcgccttt
     1441 cctcgtggca ctcgaagagt cggcggaagg gtgccgcccc ggccgattcc tgcgggcgga
     1501 cgctctcccc cggtaccgga acgcggaaaa cgcggggtgg aaattcctcg ttttcgacga
     1561 aacctccggc gcccccaaga tgcccctggg gtcgatcggc ttccgctggc aggaagagaa
     1621 ggggaagtgg aacctcgaga tgaaggacgg cctggacggt gcggagatcc ggccgctcct
     1681 cacgttcctc gagagccggg aggcggtgct gccggtcacc ttctcgggat tcgaggacgg
     1741 aaaaaccttt cggcggggcg tgccggtccg gtacgtcgaa acggagcggg ggaacgttcc
     1801 ggtgacgacg gtcttcgacc tcctgatggc gcgattcggc gtcgggcgcg ggctggaagg
     1861 ggaattcccg gccgattaca acgacgggga ggcgctgtac acgcccgcct ggcaggagcc
     1921 gtacaccggc gtgggacgcg agacggtcat ccggttcgcc agggaatggg cgtcgaccgc
     1981 ggaaaagacc gggggaaaat gcacgatcct catcggctcc ggtgcgaacc actggtatca
     2041 cgccaacctg aattaccggg ccgggatcac ggccctggtc ctgtgcggct gcgtgggggt
     2101 gaacggcggc gggctcaacc attacaccgg ccaggagaag gtcactcccg aagcctcctg
     2161 gtcgactctc gcgttcgcga aggactgggg aaagccgtcg aggctccaga acgccccctc
     2221 gttccattac gtccacagcg accagtggcg gtacgatctg ggcgtagcgg atctccctcc
     2281 cggagaatcg ccgggcgtgc cgggccggaa gcaccccgtg gagtacctgg cggactcggt
     2341 ccgcatggga tggctcccgt tcaccccgca gttcaatcgc aatcccgcgg agctggtgag
     2401 ggaagcggag gcggcggggg cgaaaagcga cgcggagatc gtcgggtggg tggtcgaaca
     2461 gctccgaagc ggaaatctcc gcttctcggt ggaggacccg gacgccccgg aaaactggcc
     2521 ccggatctgg ctgatctggc ggggaaacgc gctcctggcg agcgcgaagg gacacgaata
     2581 tttcctcaag cattacctcg gcacgcatca cagctccgta gcggacgaga ccgcgtccgg
     2641 cacggccggg gacgtgaacc ggagggagcc ggctccccgt ggaaagctgg atctcgttgt
     2701 cgacgtcaat ttccgcatgg acacttcggc gctctactcc gacgtgatcc ttcccgccgc
     2761 cacctggtac gagaaggacg acctgaacac gacggacctt cactccttca ttcacccgct
     2821 gtccgccgcg gttcccccgt gctgggaatc ccgcaccgac tgggacatct tcaagacgat
     2881 cgcgaaaaag acgagcgagc tggcgcgcct ccacggcccg gaaccgtttc gggagatcgt
     2941 cgcctccccc ctgttgcacg actctccgga cgagatcgcc cagcccgagg tgaaacgatg
     3001 gtatgagggc gagtgcgaac cggtcccggg gaagacgatg ccccatctcg cggtggtcga
     3061 acgcgactac gcgaacctgt acgaccgctt cgtctccctc ggggattccc tcagggaaaa
     3121 cgggatggag gaaaaaggga tccattggcc cgtggcggac ctctacgacg agctgctcga
     3181 gagcgccccc gtacgcgagt ggaacggccg gaattacccc tccctcgagg aggtcgtcga
     3241 cgccgcgaac gtgatcctcc acctcgcccc ggaaacgaac ggggaagcgg cctacacggc
     3301 tttccgggcc caggagcgga tcgtggggct gccgctcgcc gacctggccg aaaaacagcg
     3361 cggcacccgg taccggttcg ccgacctggt tgcccagccg cggagggtgc tcaccagccc
     3421 ctgctggtcg gggatcacca acgaagggag gccgtactcc gcctattgca tcaatgtgga
     3481 acggcgggtt ccctggcgga ccctcaccgg gcgccagcac ttctacctcg accacgaatg
     3541 gtacctcgcc ttcggcgaaa acctccccgc cttcaaatcc aaggtggacc tggaaagctc
     3601 cggcaacctc gcgaagagcc gggcggaggg aaaggcgctg gtcctccact acctcacccc
     3661 ccacgggaag tggcacatgc acaccacgtt ttccgacaat caccggatgc tcaccctctc
     3721 ccggggcatg gagccgttct ggatgaacga ccgggacgcc gcggagatcg gggtggccga
     3781 taacgactgg gtcgaagcgt acaacgacaa cggggtcgtc gtgacgaggg ccgtggtaag
     3841 cgcgcgcgtg ccgcgcggaa ccggaatcta ctaccatgcc ccggaacgca ccatttccgt
     3901 cccccggtcc cctttgagaa acaaccggag ggcggggggg cacaacagcc tgacccagac
     3961 gcggctgaaa ccggtcctca tgacgggcgg ctacgcccag ctctgctacg ctttcaacgc
     4021 ctggggtccg ccgggccccg accgggacac gtatgtttat gtccacaagc tcgaggggaa
     4081 acctcgatgg tagcgggtga aggaggacgc cggtgaacat tcgcgcgcag gtctcgatgg
     4141 tgttccacct ggacaagtgc atcggctgcc acacctgcag catcgcctgc aagaacatct
     4201 ggaccgaccg ccggggcacg gaatacatgt ggtggaacaa tgtggaaacg aagcccggca
     4261 ccggataccc caccctctgg gaggaccagg agaagtacca tggggggtgg gtgaaagagg
     4321 ccggaacggt ccggctgaag ctccacagcc gtaagagcgg gctggcgaac ctcttccaca
     4381 accccttcct ccccaccatg gacgactatt acgaaccgtg gacgtaccgg tacgaagacc
     4441 tcctgaacgc cccgaagggg gaggatcagc cgaccgcccg ccccgtctcc atgatcacgg
     4501 gaaaaccgat cgacatcgag gcgggaccga actgggacga cgacctgggc ggctcccaga
     4561 tatacgcgtc caacgatccg aacctggccg cggccacccc cgaggagcgc cgccagctct
     4621 tcgcgctgga gcggatcgtc ttcttctatc tccctcggat ctgcaatcac tgcctcaacc
     4681 ccggctgcgt ggctgcctgc cccgccgggg cggtctacaa gcgcggcgag gacggcatcg
     4741 tgctcgtgag ccaggagaag tgccgggcgt ggcgaatgtg catctcggga tgcccctaca
     4801 agaaggtcta ttacaactgg gagacgggaa aatcggaaaa gtgcattctc tgctatccgc
     4861 ggcaggagac cggccaggcc ccggcctgct tccactcctg cgtggggcgg atccgatacc
     4921 tggggatgct cctttacgac gcggaccgga tcgaacggac ggcgggaagg ccggaccggg
     4981 agatggcgga agcgcagagg gagatgatcc tcgacccgtc ggacccggag atcctccgga
     5041 aagcgcgcga ggacggcgtc cacgaggcaa tcctcgaggc cgcggtgaaa tcgccggtct
     5101 accggtacct gaaggagtgg aagctggcgc tgccgcttca cgccgggttc ggcaccctcc
     5161 ccatgctttt ctacgtcccg ccgcttctcc cggaggcagc ttccgtgcgg gagggccggt
     5221 acgatcccgg aaacgacctt ttcggctcca tcgagaaggc gcgcctgccg gtccactacc
     5281 tggcgtccct cttctccggc ggcgacgagg aggcgatcaa ggcgatctac cggaagctga
     5341 tcgcggtgcg gatttaccgc cggtcgaaaa cggtgggaga catcccggag gaagagctgg
     5401 aacgggccct cacgggagcc gggatcggcg ccggagaagc cgaggcgatc taccggatga
     5461 cctccctcgc ccccttcgag gagcggcacg tgatcccgcc cttcctgcgg gagatggcca
     5521 tcgagcagac gaccgacccg ggggtccaca agacggaaac gggggtgggc accctccgtc
     5581 ccccgaaacg ggggttgtga atggaggacg ctttcgaggc ccgttaccgg ctgtttgcgg
     5641 atctcctgtc ctgcccgacg ccgtccctcc cggcccgtgc ccgggaggga gcttcccttc
     5701 tcgcgtccgt ggacggtgac gccgcggacc tcgtggaccg gttccgtgac ttcctggagg
     5761 agaccccgcc gggccgactc gaggaaatcc acaccggcgt cttcgacctg cagccggtct
     5821 gctgtccgta cgtcggatac catctcttcg gggagcatta ccgccgcggg atcttcatgg
     5881 cccggctgcg ggaacattac cgcgaccatt ccttcccgga ggacaaggaa ctcccggatc
     5941 acatcgtcgt cctgcttcgc tacctggccg ggcgccacga cgaggagacg gaccgcgacc
     6001 tcgtgacgga gtgcctgctc ccggcggccg agaagatggt caagggattc acggacggga
     6061 cgaaccccta ccgggggatc ctcgaggctc tcgtccgggt cctgcgcagg gaggcggcat
     6121 cgagcgcgaa cgcggatccg cggaaggagg tcgaccgatg accgacctgt tcctgttcgt
     6181 cgttttccct tatgtggccc tggcgctggc cgtcgcgatg ggggtgcacc ggtacgttgc
     6241 cgaccggttt tccttctcga gcctctcttc gcaatttctg gagaaccggg ccctcttctg
     6301 gggttcggtt ccctggcact acgggatcgt atcgatcctg gttgcgcacc tcctggccgc
     6361 cctctttccc ggatcgtggg gatccctcct cggaccgccg ggccgccttt attttctgga
     6421 ggtgaccggg atggcgctgg ggctcttcac gctcgtcggc ctgctcctgc tcattgcccg
     6481 acgcgcggga aacgccagga tccgctccgt cacgacagtg atggacggga tccttctcgc
     6541 ggacctcgcc ctgcaggtcg ccaccgggct ttacatcgcc tattcctacc gctggggctc
     6601 tctctggtac ctgcacacgg cggtcccctg gctgtcctcc ctggcgcggc tgcaaccggt
     6661 aaccgccgga atcgcgcccc tcccgccggt cgtccggttc cacctgttca acgccttcat
     6721 cgtcgtgggc ctgttcccgt tcacccggct ggtgcacatc ttcacggtcc cgatcgagta
     6781 cctgtggcgc ccctggcagg tggttctctg gaaccggcgg ccgccccctt gaaccgcttc
     6841 gcgttctcga tcgatcccat accccctttt cggctcgacc tgacggcgtg gacgttgcgc
     6901 cggcgccccg gcaacatcgt ggaccgttgg gacggaaaga cgtatcggcg cgcgctggtg
     6961 gtgggaagcc atcccctgga agtggaagtg aaacaaaccg gtccccccga ggcgccgcgc
     7021 ctgaaggtcc gggcgacaag cgccgtgccg ttccccggcg aaggatccgc cgtgaggact
     7081 atcgttgagc gcatactggg agcacggatc gacctgggag ggttttaccg gttcgcggcg
     7141 ggcgatcccg cgctgggacc ggttgtcaca cggttccgcg gggcaaaacc tccccgtttc
     7201 ccgaccctgt tcgagacgct gacgaacgcc atcgcctgcc agcagctttc cctcacggtg
     7261 ggaatcctcc tgttaaaccg gatgaccgaa gcgtgcgggg ccgcgattcc cggttcggat
     7321 ccggccgtgc acgcgttccc gagcccggag gatgtcgcgg cgctggcgcc ggagacgctt
     7381 cggagcctgg ggttcaaccg gcagaaggcc cgggcgcttc tcgaactggc gggcgtcgtc
     7441 gcagggaaac ggatcgacct cgacggcatc gcctccctgg aagacgaagc agcggttgac
     7501 cggctgcagg aactccgggg agtggggcgc tggacggccg agtacgtcct gctgcggggc
     7561 ctggggcgaa tccacgtctt cccgggcgac gacgtgggag cgcgcaacca cctcttgcgc
     7621 tggttgggaa agaggaagac cctgggttac gaagccgtcc gcagcgcgct ttcccgatgg
     7681 aagccgtacg cgggcctcgt ctacttccat cttctcctca agggactctc ggaagcgggc
     7741 cggtttccct gacgttgggc cgtccccggg aagcgttcct tctcctctca ggaggaatcc
     7801 ccgaacaatc aacggaaggg agattgacat cggaagataa ataggactag aatggtcctg
     7861 tatctgggag gggatgagag aatgcttcgc gtgagcaagt tggcggatta cagcaccttg
     7921 gcgatcagct acctggcgtt ggagcccggg gaaggccgta ccgcgaagga aattgccgtt
     7981 gcgatccgcg tggcggcgcc cacggtgagc aaaatcctaa aaatattggc gcggaaagga
     8041 ctgctggttt ctctccgcgg cgccgggggc ggctaccgtc tggccctccg tcgcgaacag
     8101 atatccgtgg cccgggtgat tgaggctgtg gaaggaccgt tcgcattcac cgaatgcagc
     8161 ggagcgccgt gccactgcat acgcgagccg aattgcccca ttcggagcaa ttggcaacgt
     8221 atcaaccggg acgtgcggaa ggtgctcgaa agtgtaactc tcgcggacat ggtccggccc
     8281 gcacgatccc gcccggatcc gaccgtcccg tcccaaaccc gttccatgac gaagcggact
     8341 caagcgacaa gagccgggcg ggtttcggcg ccgacaaacc gaggcggcaa tgagtgattc
     8401 gacaagagac gtcaaagagc ggttgaatcg ggaataccgg tggggatttg tcaccccgat
     8461 ggaggaggac tccgtcccca agggcctcaa cgaaggcatc atccgcacga tctcggccag
     8521 gaaaaacgaa ccggaatttc tgctcgagca gcgcctgaaa gcctaccgtc attggctgac
     8581 gatgacggag ccgaaatggt cgaatgtgca ttatcccccc atcgactacc aggacatcgt
     8641 cttctattcg gcgcccagga cgaacaaagc gaagcccacg agcctggacg aagtagaccc
     8701 ggtcctgctc aatacctacg agaagctggg cattccgctc agggaacggg aactgttggc
     8761 cggggtggcg gtggacgcca tattcgacag cgtgtcggtc gctacgactc atcaaaaaga
     8821 gctggccgaa caagggatca tcttttgctc cttttcggaa gcggtgcgga gccatcccgg
     8881 tctggtgaag aagtacctcg ggtcggttgt accccacacc gacaatttct tcgcgtcgct
     8941 gaactccgcc gtgttcagcg atgggtcgtt cgtctatatt cctcccggtg tgcgctgccc
     9001 catggagctt tccacctact tccgcatcaa tgcggcggac accggccagt tcgagcgtac
     9061 cctgatcgtc gccgacgcag gggcttacgt gagttacctc gaaggttgca cggcgccgag
     9121 gcgggacgag aaccagctcc atgccgcggt agtggaactg atcgcgctgg agaatgcgga
     9181 gataaaatac tccacggtgc agaactggta cccaggggac aaggaaggga aaggcggcat
     9241 ttacaatttc gtcacc
//
LOCUS       DASVJL010000244         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_883354, whole genome shotgun sequence.
ACCESSION   DASVJL010000244 DASVJL010000000
VERSION     DASVJL010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883354"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VF357_04975"
     CDS             complement(<1..769)
                     /locus_tag="VF357_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7523530.1"
                     /translation="MLFLKSRRREQEEVLSAHQKELSGLKRDLQARDDEAGELRNKIE
                     GMKAEIAARERSAAESVQRVKKYSDHVENLHVLAKKLTSINNLEEIYRFVADACHDVF
                     QFDRVNILIADETNMMRCVETRGNLNEPIEKIMVPIHQDAGALYWAYAESKVIVLDLG
                     TKVAPKEIPQKYYMKKPCSEIKAFRSTSCIVGALLGRTKPVGVFAIDKKLKKLRVTED
                     DISLVNLLQDIASYAIQNIQTVNELKVHQSELYDLIRI"
     gene            988..2157
                     /locus_tag="VF357_04980"
     CDS             988..2157
                     /locus_tag="VF357_04980"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEX7523531.1"
                     /translation="MKLLVLNSGSSSIKYRLFQMESMAVLRAGAVDEIGEAGASAVRD
                     HREGFTRVMSDLSDSGAIREASDLFGIGHRVVHGGERFREPTRVDAGVLEAIREMIPF
                     APLQNPGDLLGIEVALATFPGVPEVAVFDTAFHQTMPPRAFRYALPRDLYDAHRVRRY
                     GFHGTSHAHVARRAAELLGKPTGSLNLITLHLGNGASAAAILEGKSVDTSMGMTPLEG
                     LIMGTRCGDLDPAVPFFLGTVTGKNPEELLALLNEESGLKGICGANDMREVHRRTAAG
                     EPSAELAIDMYSYRIRKYIGAYTAVLGRVDALVFTGGIGENDAEVRRRACEGMEKLGI
                     AVDEAKNGSPSSVPREIQREGMPVKVLVIPTNEELEIALQTFACIRNNVAPEEPG"
     gene            complement(2167..>3017)
                     /locus_tag="VF357_04985"
     CDS             complement(2167..>3017)
                     /locus_tag="VF357_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523532.1"
                     /translation="MPLDWDGKIRMDCSSPFAMAGLIAMRDRFDVAFGNDTDADRHGI
                     VTRTSGLLNPNHYLSVAIDHLFRRRGGWRSDAGIGKTVVSSGMIDRVAARLSRRLHEV
                     PVGFKWFVQGLSDGTLGFGGEESAGASFLREDGTAWSTDKDGLILGLLAAEMMAITGR
                     DPGELYEELTREFGAPVYERIDAPATKEQKAALSKLSPSQVTADTLAGERIEAMLTAA
                     PGNGAAIGGLKVVTANGWFAARPSGTEDVYKLYAESFVGADHLRQIQEEARDLIARVF
                     SSPGRG"
BASE COUNT          503 a          996 c          955 g          563 t
ORIGIN      
        1 tgatgcggat gagatcgtag agttcgctct ggtggacctt cagttcattt acggtctgga
       61 tgttctggat ggcataggat gcgatgtcct gaagcaggtt gacaagactg atgtcgtcct
      121 cggtcacgcg gagcttcttg agcttcttgt cgatcgcgaa aacaccgacc ggtttcgtcc
      181 tccccaggag cgcgccgacg atgcacgacg tggatcggaa cgccttgatc tccgaacacg
      241 gcttcttcat gtaatacttc tgcggaattt ccttcggagc caccttggtt ccaaggtcga
      301 ggacgatgac cttgctttcg gcgtaggccc agtacagcgc gccggcgtcc tggtggatgg
      361 gaaccatgat cttctcgatc ggttcgttca gattgccgcg cgtctcgacg cagcgcatca
      421 tgttggtctc atcggcaatc aggatgttca cccggtcgaa ctggaacacg tcgtggcagg
      481 cgtccgccac gaagcgatag atctcttcga ggttgttgat ggaggtgagt ttcttcgcga
      541 ggacgtgcag gttctccacg tggtccgagt attttttgac gcgctgcacg gactccgccg
      601 cggagcgctc ccgggcggcg atctccgcct tcatcccttc gatcttgttc cggagctcac
      661 cggcctcgtc gtcccgggcc tgcaggtccc ttttcaggcc cgacagttcc ttttggtgcg
      721 cggaaaggac ttcctcctgc tcccgccttc gactcttaag aaataacatg ccgcacctct
      781 tgaatcttgc cattcttatc ggcgcatttc ttatcggcag gatcggcggg aactttaacc
      841 gtgtttgaaa atttctccgg tcgttcgatc cctgcgccac ccttcaggaa acggattcgc
      901 cccgccggaa ccaggcgacg gtcgcaggag gtatactttc cactggcatc gggcatctcc
      961 ccaaccggtg tacgggaggg aaccgcattg aagctcctgg tcctgaactc cgggagttcg
     1021 tcgatcaagt accggctctt ccaaatggaa tcgatggccg tgctgcgcgc cggcgccgtg
     1081 gacgagatcg gcgaggccgg cgcgtccgca gtccgggatc accgggaagg gttcacgcgc
     1141 gtgatgtccg acctttcgga ttcgggtgcg atccgggaag cgtccgacct cttcggcatc
     1201 gggcaccggg tcgtccacgg cggcgagcgt ttccgggagc cgacgcgggt ggacgccggg
     1261 gtcctcgagg cgatccggga gatgatcccc ttcgccccgc tgcaaaaccc cggcgacctc
     1321 ctggggatcg aggtcgccct cgcgacgttc ccgggggtgc cggaggtggc ggtcttcgac
     1381 accgcctttc accagacgat gcctccccgc gccttccgct acgccctccc ccgcgatctt
     1441 tacgacgccc atcgcgtgcg gcgctacggc ttccacggga cctcgcacgc ccacgtcgcc
     1501 cggcgcgccg ccgaactcct cggcaaaccg acgggatccc tgaacctgat caccctccat
     1561 ctggggaacg gcgcgagcgc ggccgcgatc ctggagggga agagcgtcga cacttcgatg
     1621 gggatgaccc cgctggaggg actgatcatg gggacccgct gcggcgacct ggaccccgcg
     1681 gtacccttct tcctcgggac ggtgaccggg aaaaatcccg aggaactcct ggccctgctg
     1741 aacgaggaga gcgggctgaa agggatctgc ggggcgaacg acatgcggga agtgcaccgg
     1801 aggaccgcgg cgggggagcc atccgcggag cttgcgatcg atatgtacag ttaccggatc
     1861 aggaaatata tcggcgccta caccgccgtc cttggccggg tcgacgcgct ggtgttcacc
     1921 ggggggatcg gcgagaacga cgccgaggtg cggcggcggg cgtgcgaagg gatggagaag
     1981 ctcgggatcg ccgtcgacga agcgaaaaac ggttcgccct cttcggtccc ccgggagatc
     2041 cagcgggaag ggatgccggt caaggtcctc gtcatcccga cgaacgagga gctcgagatc
     2101 gccctgcaga ccttcgcgtg tatcaggaat aacgtcgcgc cggaggaacc gggatgaagt
     2161 ggaaccttac ccccgacccg gggaggagaa gacccgcgca atgaggtccc tggcctcctc
     2221 ctggatttgc cgcaggtggt ccgcgccgac gaagctctcg gcgtacagct tgtagacgtc
     2281 ctcggtcccc gaggggcggg cggcgaacca gccgttcgcg gtgaccacct tcaggccccc
     2341 gatcgcagcg ccgttcccgg gggccgccgt gagcatcgcc tcgatccgct cccctgccaa
     2401 cgtatccgcg gtcacctgag aaggcgacag cttcgacagc gcggctttct gctccttcgt
     2461 cgcgggggcg tcgatccgct cgtacacggg cgcgccgaat tcccgggtca gctcctcgta
     2521 cagctcgccc gggtccctcc cggtgatcgc catcatctcc gccgcgagca gcccgaggat
     2581 cagcccgtcc ttgtccgtgc tccacgccgt tccgtcctcc cgcaggaacg aggcccccgc
     2641 gctctcctcg ccgccgaacc cgagggtgcc gtccgacagc ccctggacga accatttgaa
     2701 ccccaccggc acctcgtgca gccggcgcga caggcgtgcc gccacgcgat cgatcatccc
     2761 gctgctcacc acggtcttcc cgatcccggc atcgcttcgc cacccgcctc gccgccggaa
     2821 gagatggtcg atcgccacgg agaggtaatg gttggggttg agaagtccgg aagtacgcgt
     2881 cacgatgccg tgccggtcgg cgtccgtgtc gtttccgaag gcgacgtcga accggtcgcg
     2941 catcgcgatc agcccggcca tcgcgaacgg ggaggagcag tccatccgga tcttcccgtc
     3001 ccaatcgagc ggcatga
//
LOCUS       DASVJL010000245         9689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_976237, whole genome shotgun sequence.
ACCESSION   DASVJL010000245 DASVJL010000000
VERSION     DASVJL010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9689
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976237"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VF357_04990"
     CDS             <1..212
                     /locus_tag="VF357_04990"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523533.1"
                     /translation="VTRGVVTESGSEALVEGARDEVLRAIEEIGVDARTDTAEMQTAI
                     RRVVRRFFNKRIDRKPMIVPVLLEL"
     gene            215..2362
                     /locus_tag="VF357_04995"
     CDS             215..2362
                     /locus_tag="VF357_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX7523534.1"
                     /translation="MADIGKIRRETVAVLFLAAGVVLSVALVSFHQMDPSLSTASSPG
                     AEVRNWAGWGGAIFSDLLLQLFGVGAVGFPLLCLLLAYWTYRGEGILGKWARTAGGLL
                     AVCSVLGILSFFTGHVSVMGQDVFLPGVVGDLLGVHFLGRVAGTAGGLILLVALLLFS
                     LMLVTGLPLSGLPALVRKDSSPEKVRERIKEKLAPHETWGEERQTPEAREEAAPPRVV
                     ARRPVPEEAAPPRTAGKAFILPPLDLLEPSKGVEEGVDEETLRENAQALLTKLAEHGI
                     DGQITEIRTGPLVTMYEFRPAPGIKANRVSAMADDLALAMRCESVRVVPNIPGKGVMG
                     FEIPNSRRAPISLRELLGCPAYASAVPALSLAMGKDIFGDPVVRDLGKMPHLLIAGAT
                     GSGKSVALHTMILSILFRATPDEVRLILVDPKMLELSLYDGIPHLYHPVVTQPRDAAQ
                     VLKWAVGEMRGRYQLMMENGVRHIDAFNQFVEKRLRSGGRSKAEGEGDDLVKLPYIVI
                     LIDELADLMMTSASRREVEDSITQLTQMARAAGIHLIFATQRPSVDVLTGVIKANFPS
                     RVSFKVISQFDSRTILDQSGAETLLGFGDMLFLQPGVGGIVRVHCPYVGEGEIQRVVE
                     HLKAQGPPVYDSAITAPPSTEDPDPSRDEMFDAAVEEVVRAGRASVSFLQRRLKIGFN
                     RAARIVEEMERQGIVGPAEGGKQREVYVTRKEE"
     gene            2520..3209
                     /locus_tag="VF357_05000"
     CDS             2520..3209
                     /locus_tag="VF357_05000"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX7523535.1"
                     /translation="MMHSRSESHSACALLLACALLLLPLSVSRAAGTESAGEALLRRV
                     GQRYAAAHTLSAKFRQEIPLQNVGIVRKASGTVYFGRPLKMRWDYEGSEAQLFLADGR
                     YFYFRPAGSSQVIRRGVDEKGLGGKIPLLLLFGKGEITDLFRVDAADPRNGGEETVLH
                     LSPLADGAPEVRRIDLVVGTGDALIREIHVFDRLGGENHIYLTDVTVNPSLPSDLFRF
                     RKPAGVSVVDG"
     gene            3227..4591
                     /gene="rimO"
                     /locus_tag="VF357_05005"
     CDS             3227..4591
                     /gene="rimO"
                     /locus_tag="VF357_05005"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX7523536.1"
                     /translation="MPTVRIHNLGCGKNAVDAEVMAGLLSEGGFLVVPGGRADAAVLN
                     TCGFVRAAKEESIEAILSLAAEKRRGRIRRLIVAGCMARRYREELPELLPEVDLFLGP
                     GDIPDLPGRLSSMLLEEGPSPGGSAQRSLSGGGALPDEAYGHRLPEAGTGSAFLKILE
                     GCDNRCAYCAIPAIRGPLRSRDRESLLSEARLLVRRGARELNLIGQDITAYGLDRGER
                     GGLVSLVRALCAVRGVRWVRLLYLYPSRVDDGIVDLLRSEEKVCRYLDIPVQHIAPGI
                     LRRMGRTYGPDAIYRMLDRLRTGVPGLFLRTSLIVGFPGETRAAFDRLLRFVDETRWD
                     YLGVFPYSREEGTPAFRMPSQVPERTKEERARRVRDAQADLLAARNASRIGETLDVLV
                     EKTGARGKAVGRHRGQAPEVDGSVILSGFDGKPGSIVRARVTGAKEWDLRGVVVRSRV
                     SESD"
     gene            4654..5052
                     /locus_tag="VF357_05010"
     CDS             4654..5052
                     /locus_tag="VF357_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX7523537.1"
                     /translation="MKTIKVMLLKKREDLVLEIARRSKASTESSVQDIGDIVDSVSEE
                     RTRELDLILTDREKRKLAQIDDALDRIEESTYGLCEECGVKIPKARLKVLPFAKYCVE
                     CQEKNEREEKYTREESEDGIRKVPVADVEE"
     gene            complement(5145..6167)
                     /gene="mltG"
                     /locus_tag="VF357_05015"
     CDS             complement(5145..6167)
                     /gene="mltG"
                     /locus_tag="VF357_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX7523538.1"
                     /translation="MNAAADRSRRIGWGVALAVLGVALLYVFFLFDTPPTGNWEGKLV
                     LVPKGSPLPGMLGILQEDGILPHPLAFRTLVLLTNTGRRLHYGEYAFTTPPSAFEVWR
                     RLTRGDVIKYEVTVPPGANLYDIAKLLEGEKLVAAEAFLATVASPAVLRRLEIPGESA
                     EGYLFPDGYIFVKPVTPEEILETMVRQFRRKIPPDAEKRAGEAGLSLHQVVTIASIIE
                     KETGVEGEKPIVSAVIRRRLALDMPLQMDPTVIYGVKRFDGTVTRKDLRTTGPYNTYL
                     NRGLPPGPIANPGLASLAAALNPSKTEYLYFVSRNDGSHMFSRTLPEHLRAVERFRRA
                     AREDEG"
     gene            complement(6167..6601)
                     /gene="ruvX"
                     /locus_tag="VF357_05020"
     CDS             complement(6167..6601)
                     /gene="ruvX"
                     /locus_tag="VF357_05020"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX7523539.1"
                     /translation="MGDVVTGERVLGLDYGSRRIGVAVSDPLGLTVQPLPPIRREGDK
                     KDIVALARLAAEMGVTSVVLGLPLLLNGDEGPAAVRARTFGERLQAETSLPVTMWDER
                     LTSVQSERHLIASGVRREDRKGIRDSLSAMFLLQAALDSRRR"
     gene            6773..7351
                     /locus_tag="VF357_05025"
     CDS             6773..7351
                     /locus_tag="VF357_05025"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7523540.1"
                     /translation="MIERIEEVLGKLLSDPSAAVREAASGSMDRTLAKRSLEESRSRI
                     LGGTVEEKLRAIYAAAELGGSEGVSLLLQALSDRDAEIRGAAVRALSPFPSPGVIKSL
                     WEMLPRERGVVLGNLIETLGASGRRELAPHVEKFLDHPEPEVRAKAVKAYSRLCDGPG
                     WEKILPRAGDTNETVRAAVAEALGGWTSSPRS"
     gene            complement(7359..8747)
                     /locus_tag="VF357_05030"
     CDS             complement(7359..8747)
                     /locus_tag="VF357_05030"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX7523541.1"
                     /translation="MDRRQMESAIAGVVRYLGQSLKNRGLYPATHPLVLTPVEKCHME
                     LTPFFADRSELALTVSDGTLVLEGVPIFQLTSSLELFMARLGAIGLPAVIFERGVSVE
                     DIELFVRFLHETKEQGLPIPEIKERLARWGVTHIRVTATDEEEKDDFTLAREIYGNAL
                     NVVVRALKDVRYGKTPDGAESDRAVREMSGMVSRNRDAMMALTLIKNFDEYTYNHSVN
                     VSVFSLAMAEALGLPEAERIGIGVAGLLHDVGKTQLALDLIRKPGTLTVEEFEEIKKH
                     PEEGFAILGKMTHIQESTRAVVREHHMRFDRTGYPRPEPEYRMNPHSFVIAVADCHDA
                     LTTMRSYQKARTPQQTFEIMRKLAGKSLDPDLVELMERSLGIYPVGTMVRLSTMEVGV
                     VTGTLDGEKGEPKVAILFDRSGKPLATPQGVDLKEFDPSSGRPRRMILGIVNPLMHPP
                     VSMIGILQAVSG"
     gene            complement(8763..>9689)
                     /locus_tag="VF357_05035"
     CDS             complement(8763..>9689)
                     /locus_tag="VF357_05035"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7523542.1"
                     /translation="RFLLAERAERKIEFSTQAMTIFIRHIEFPPGGSDEIAGLARLGI
                     KEVASEELVAHYIGLLKKRGARGREETDAVLVALEERSVGPLLQALAEEEDLLVRKAI
                     VEIVTRIGRIAVPTILENLTDSRWYMVRNMITVLGTLGMPDLAPHVAATLPHPDLRVK
                     KEAIKALSRIPHPSAVATLCDLSFFPEETVALTATAALSSKKETEAVVALYRRAAAKL
                     FLYPNYRLAHEAIDSLRTIGTDEAVTALEEILALRALWSTKKFRAMKFHALRSISKIK
                     GERSKEVLERARRSADRSLRIEAERIIARRVP"
BASE COUNT         1534 a         3172 c         3310 g         1673 t
ORIGIN      
        1 tcgtcacacg cggcgtcgtg accgaaagcg gttccgaggc gctcgtcgag ggggcgcggg
       61 acgaggtgct ccgcgcgatc gaggagatcg gggtcgacgc gcggaccgac acggcggaga
      121 tgcagaccgc gatccggcgg gtggtgcgcc gcttcttcaa caagcgcatc gatcggaaac
      181 cgatgatcgt ccccgtgctc ctggagcttt gaccttggcc gatatcggaa agatccgtcg
      241 ggaaaccgtg gcggtccttt tcctcgccgc gggggtcgtc ctctccgtcg cgctggtgtc
      301 gtttcaccag atggatccct ccctctccac cgcaagctcc ccgggagccg aggtgcggaa
      361 ctgggccggc tggggcgggg cgatcttttc ggacctgctc ctccagctgt tcggcgtcgg
      421 cgccgtcgga ttccccctcc tgtgccttct cctcgcctac tggacgtacc ggggcgaagg
      481 aatcctcggg aaatgggcgc gcaccgccgg ggggctcctc gcggtctgct ccgtcctcgg
      541 gatcctcagt ttcttcacgg gacatgtctc cgtcatgggg caggacgtct tcctgccggg
      601 ggtcgtcggc gacctgctcg gggtccattt cctcgggagg gtggcgggca ccgcgggcgg
      661 gctcatcctg ctggtcgccc tcctcctgtt ctctctgatg ctggtcaccg gtcttccatt
      721 gagcggcctg ccggcgctgg tccgcaagga ttcgtcgccg gaaaaggtcc gcgagaggat
      781 caaggagaag ctcgccccgc acgagacgtg gggggaggag cgccagacgc cggaagccag
      841 ggaagaagcc gcccccccgc gggtcgtagc gcggaggccc gtccccgagg aggccgcgcc
      901 cccccggact gccgggaagg cgtttatcct gccaccgctc gatctgctcg aaccgtccaa
      961 gggcgtcgag gagggggtcg acgaggagac gctgcgggag aacgcacagg cgcttctgac
     1021 caaactcgcg gagcacggca tcgacggcca gatcaccgag attcgaaccg gccccctggt
     1081 caccatgtac gagttccgac ccgcgccggg aatcaaggcg aaccgggtat cggcgatggc
     1141 ggacgacctt gcgctggcga tgcggtgcga gtcggtgcgc gtggtaccca acatccccgg
     1201 aaagggggtc atgggattcg agattcccaa cagccgcagg gccccgatct cccttcggga
     1261 gctgctcggg tgccccgcct acgcctccgc cgtccccgcg ctgtcgctgg caatggggaa
     1321 ggacatcttc ggcgatccgg tggtcaggga cctcggaaag atgccccacc tcctgatcgc
     1381 cggcgccacc gggtcgggaa agagcgtggc gctccacaca atgatcctgt cgatcctctt
     1441 ccgcgcgacg cccgacgagg tccggctcat cctcgtcgac ccgaagatgc tcgagctgtc
     1501 gctctacgac gggatcccgc acctgtatca cccggtggtt acccagcctc gcgacgcggc
     1561 ccaggttcta aagtgggcgg tgggggagat gcgcgggcgg taccagttga tgatggaaaa
     1621 cggagtccgc cacatcgacg ccttcaacca gttcgtcgag aagcggctcc gctcgggcgg
     1681 caggtcgaag gcggaagggg agggcgacga ccttgtgaag ctcccctaca tcgtcatcct
     1741 catcgacgaa ctcgccgacc tgatgatgac ctccgcgtcc cgccgggagg tggaggattc
     1801 gatcactcag ctcacgcaga tggcgcgggc ggcggggatc cacctgattt tcgcgaccca
     1861 gcggccctcc gtggacgtgc tgacgggcgt gatcaaggcg aacttcccgt ctcgggtctc
     1921 cttcaaggtg atctcccagt tcgactcccg gacgatcctc gaccaatcgg gggcggagac
     1981 gctcctcggg ttcggggaca tgctcttcct ccagcccggc gtcggcggga tcgtgcgggt
     2041 gcactgcccc tacgtcgggg agggcgagat ccagcgcgtc gtggagcatt tgaaggccca
     2101 gggcccgccg gtctacgact ccgcgatcac ggcccccccg tccacggagg atcccgaccc
     2161 gtcgcgcgac gagatgttcg acgccgccgt cgaggaagtc gtccgcgccg gccgcgcctc
     2221 cgtctccttt ctccagcgcc gcctcaagat cgggttcaac cgcgcggcgc ggatcgtcga
     2281 ggagatggag cggcagggga tcgtcgggcc cgccgagggg ggaaaacagc gcgaggtcta
     2341 cgtcacccgg aaagaggagt gaaccccctg gcggccgacc gcacagcgga gcgcacccca
     2401 ctctcgtacg aaatgcgctc cctggggtac cccttggcaa gacatgaggg gcacgacgca
     2461 gggttccgaa gatcccggga aggactcatg acgctgatat ggagcatact gaacccatga
     2521 tgatgcactc ccggtccgaa tcgcattccg catgcgcgct cctgctcgcc tgcgcgcttc
     2581 tcctgctgcc tctgtcggtg agccgggcgg cagggacgga aagcgccggt gaggcgctcc
     2641 tgcggcgggt cggccagcgg tacgccgccg cgcacaccct gtccgcgaag ttccggcagg
     2701 agattccgct ccagaacgtc gggatcgtac ggaaggcgtc cggaaccgtg tacttcggcc
     2761 ggccgctcaa gatgcggtgg gactacgagg gctccgaggc gcagctgttc ctcgccgacg
     2821 gccggtactt ttacttccgc ccggcgggat cgtcgcaggt gatccgccgc ggcgtggacg
     2881 agaaggggct cggcgggaag atcccgttgc tgctgctgtt cggcaaaggg gagatcacgg
     2941 acctctttcg cgtggacgcc gccgacccgc gcaacggcgg cgaggagacg gtcctgcacc
     3001 tttctcccct ggccgacggg gctcccgagg tccgtcggat cgacctcgtc gtcggtacgg
     3061 gggacgcgct catccgcgag attcacgtct tcgaccggct gggcggggag aaccacatct
     3121 accttaccga cgtgacggtc aatccttccc ttccctccga tcttttccgg tttcggaagc
     3181 cggccggggt ctccgtggtg gacgggtgac gcgggctgcg cggaaggtgc cgacggttcg
     3241 aatccataac ctcgggtgcg gaaaaaacgc cgtggatgca gaggtcatgg caggcctcct
     3301 gtcggagggg ggatttctcg tcgtgcccgg cggccgggct gacgccgcgg ttctgaacac
     3361 ctgcgggttc gtccgggccg ccaaggaaga gtcgatcgaa gcgatcctct ccctggcggc
     3421 ggagaagcgg cggggacgga tacggcgcct gatcgtcgcc ggttgcatgg cgcggcggta
     3481 tcgggaggag ctcccggaac ttctccccga agtcgacctg ttcctcggcc ccggcgacat
     3541 cccggatctc ccgggccgcc tctcctcgat gctgttggag gaaggaccgt cgccgggcgg
     3601 ttccgcgcag cgctccctgt cgggtggcgg ggcgcttccc gacgaagcgt acggccatcg
     3661 tctccccgag gccggcaccg ggtcggcgtt cctgaaaatc ctggaagggt gcgacaaccg
     3721 gtgcgcctac tgcgcgatcc cggcgattcg gggccccctg cggagccggg accgggagtc
     3781 gcttctctcg gaggcgaggc tcctggtgcg ccggggcgcc cgggagctga acctcatcgg
     3841 gcaggacatc accgcgtacg gcctggaccg cggggagagg ggggggctcg tctccctggt
     3901 gcgcgccctc tgcgccgtcc ggggcgtccg ttgggtccgc ctcctctatc tttacccctc
     3961 ccgcgtcgac gacgggatcg tcgatctcct gcgatcggag gagaaggtat gccggtacct
     4021 ggacattccc gtccagcaca tcgccccggg gatcctgcgc cggatggggc ggacgtacgg
     4081 gcccgatgcc atttaccgga tgctcgaccg gctccggacc ggggttcccg gcctcttcct
     4141 gcggacgtcc ctgatcgtcg gtttccccgg ggagacgcgg gccgccttcg accgcctgct
     4201 ccgtttcgtc gatgaaaccc ggtgggacta cctcggcgtc ttcccctact cgcgggagga
     4261 ggggaccccc gcgttccgga tgccgtcgca ggtcccggag cggacgaagg aagagcgcgc
     4321 acgccgggtg cgcgacgccc aggccgacct cctcgccgca cgcaacgcgt cgcggatcgg
     4381 ggaaaccctc gatgtgctcg tggaaaaaac cggcgcccgc gggaaggccg tcggccgcca
     4441 ccggggccag gcgccggagg tcgacggctc cgtgatcctt tccggattcg acgggaaacc
     4501 gggctccatc gtccgcgccc gtgtgaccgg tgcgaaggag tgggatttgc gcggggtcgt
     4561 cgtgcgctcc cgggtttccg aatctgattg actccggtcg gctcccgggt atactaattc
     4621 aattttattt acctggcaga ggcgggcccc gacatgaaga cgatcaaggt gatgttgctg
     4681 aagaagcggg aggatctcgt tctggaaatc gccaggcgct ccaaggcgag cacggaatct
     4741 tcggtgcagg acatcggcga tatcgtcgac tcggtgtccg aggagcggac ccgcgagctg
     4801 gatctgattc tgaccgaccg tgagaagaga aagctggccc agatcgacga cgctctcgac
     4861 cggatcgagg aaagcaccta tgggctttgc gaggagtgcg gcgtcaagat cccgaaggcg
     4921 cgtctcaagg tgctgccgtt cgcgaaatat tgcgtggagt gccaggagaa gaacgagcgg
     4981 gaggagaagt acacccgcga ggaatcggag gacgggatcc ggaaagtccc ggtggcggac
     5041 gtcgaggagt agtgcccctc atgccgatca aggaataacg tatcggctgt cggggtaccc
     5101 cagggagtgg atatcgtccg ggagtggggc gggacgccct cgctttatcc ctcgtcctcc
     5161 cgcgcggcgc ggcggaaccg ctccacggcg cggagatgct ccggaagcgt ccgcgagaac
     5221 atgtgggacc cgtcgttcct cgacacgaag tagaggtact ccgtcttcga cggattcagg
     5281 gcggcggcga gcgacgcgag ccccgggttg gcgatcggcc ccgggggcag cccccggttc
     5341 aggtaggtgt tgtacggtcc cgtcgtccga aggtccttcc gcgtcaccgt cccgtcgaat
     5401 cgcttcaccc cgtagatcac cgtcgggtcc atctggagcg gcatgtcgag ggccaggcgt
     5461 ctccggatga ccgccgacac gatcggcttc tccccctcca ccccggtctc cttctcgatg
     5521 atggaggcga tcgtcacgac ctggtgcagg gaaaggcccg cctcccccgc ccgcttttcc
     5581 gcatccgggg gaattttcct gcggaactgc cgcaccatgg tttcgaggat ctcctccggc
     5641 gtgacgggct tcacgaagat gtatccgtcg gggaagagat acccctctgc gctctccccc
     5701 gggatctcca gtcgccggag aacggcgggc gaggcgaccg tggcgaggaa cgcctccgcc
     5761 gcgaccagct tttccccttc cagcagcttc gcgatgtcgt agaggttcgc tcccgggggc
     5821 accgtcacct cgtacttgat gacgtccccg cgggtcagtc tccgccatac ctcgaaggcc
     5881 gacgggggcg tcgtgaaggc gtactcgccg tagtgaagcc gccgcccggt gttcgtgagg
     5941 agcaccagcg tgcggaatgc cagcgggtgg gggaggatcc cgtcctcctg gaggattccg
     6001 agcatcccgg gaagcgggct gcccttcggg acgagcacca gtttcccctc ccagtttccg
     6061 gtcgggggcg tgtcgaagag gaagaagacg tacaggagcg ccacccccag gactgcgagg
     6121 gcgacgcccc agccgatgcg ccgggaacgg tcagcggcgg cgttcattac cgtcgccgcg
     6181 agtccagggc ggcctggagc aggaacatgg ccgagaggct gtcccggatc cccttccggt
     6241 cctccctccg cacgccggac gcgatgaggt gccgttcgga ctggactgat gtcagccgct
     6301 cgtcccacat cgtcaccggc agggaggtct ccgcctgtaa tcgttcgccg aaggtcctcg
     6361 cccgtaccgc cgcgggtccc tcgtcgccat tgaggagaag ggggaggccg agcaccaccg
     6421 acgtcacccc catttccgcc gcgaggcggg cgagggcgac gatatccttt ttatcccctt
     6481 ccctccggat cggggggagc ggttgtacgg tcaacccgag aggatcggat acggccaccc
     6541 cgatccggcg gctgccgtaa tccagcccga gaaccctctc cccggtcacg acatctccca
     6601 tggttccctc caaagtgagt gtacgttccg cccgccgcca tcgtcaactg gccggattga
     6661 cgaaccgatt cccctccggt aagactagtg ttgcgagacg caacactgga aggtacgggg
     6721 atcggggaca gtcgtccctg cggcactcga gaaaaggcgg gggaaacgga aaatgatcga
     6781 gcggatcgag gaggtgttgg gcaagctgct gtcggaccct tccgcggcgg tgcgggaagc
     6841 cgcgtccggg tcgatggacc gaaccctcgc gaagcggtcg ttggaggagt cccggtccag
     6901 gatactcggc ggcaccgtcg aggagaagct ccgtgcgatc tacgccgccg cggaattggg
     6961 cggatccgaa ggggtttccc ttctcctgca ggcgctttcg gaccgggacg cggagatccg
     7021 cggggcggcc gtgcgggcgt tgtccccgtt cccctcgccg ggcgtcatca agtccctgtg
     7081 ggagatgctc ccgcgggagc ggggagtggt gctcgggaac ctcatcgaga cgctgggcgc
     7141 ttccggacgg agggaactcg cccctcatgt cgaaaagttc ctcgaccacc ccgaaccgga
     7201 ggtccgggcg aaggcggtga aggcgtactc ccgcttgtgc gacggcccgg ggtgggaaaa
     7261 gatcctgccg cgcgccgggg acacgaacga aaccgttcgc gccgcggtcg cggaggcgct
     7321 ggggggctgg acgtcctccc cgcgctcctg agcaggcgtt accccgaaac ggcctgcagg
     7381 attccgatca tcgataccgg cgggtgcatc agcgggttga cgatcccgag gatcatccgg
     7441 cggggcctcc ccgaggacgg gtcgaattcc ttcaggtcca ctccctgcgg ggtggccagc
     7501 ggcttccccg accggtcgaa caaaatcgcc actttcggct cgcccttttc cccgtccagg
     7561 gttccggtca cgaccccgac ttccatcgtg ctcagccgta ccatggtgcc cacggggtag
     7621 atcccgaggg accgctccat cagctccaca agatccggat cgagcgattt ccccgccagc
     7681 ttccgcatga tctcgaacgt ctgctgcggt gtccgcgcct tctggtagga gcgcatcgtc
     7741 gtcagcgcgt cgtggcagtc ggcgaccgcg atgacgaagg aatgcgggtt catccggtac
     7801 tccggctcgg gtcgcgggta gccggtgcgg tcgaaccgca tgtggtgttc cctcacgacg
     7861 gcgcgcgtcg attcctggat gtgggtcatc ttcccgagga tggcgaaccc ttcctcgggg
     7921 tgcttcttga tctcctcgaa ctcctcgacc gtcaaagttc cgggtttccg gatgaggtcg
     7981 agggcgagct gcgtctttcc gacgtcgtgg agcaggccgg cgaccccgat accgatccgt
     8041 tccgcctcgg gcagtccgag ggcctcggcc atggccaggg agaacacgga gacgttcacc
     8101 gaatggttgt aggtatattc gtcgaagttc ttgatcaggg tcagggccat catggcgtcg
     8161 cggttccgcg agaccatccc gctcatctcc ctgaccgccc ggtcggattc cgcgccgtcg
     8221 ggcgttttcc cgtaccgcac gtccttcagg gcccggacca cgacattcag ggcatttccg
     8281 taaatctccc gggcgagggt gaagtcgtcc ttctcctctt cgtcggtcgc ggtgacccgg
     8341 atgtgggtga ccccccagcg ggcgaggcgc tccttgatct ccgggatcgg cagcccctgc
     8401 tccttcgtct cgtgcaggaa ccggacgaac agctcgatgt cttcgaccga gacgccgcgc
     8461 tcgaagatga cggcggggag cccgatggcc ccgagccgcg ccatgaagag ctcgagggac
     8521 gaggtcaact ggaagatggg gaccccctcg aggacaaggg tcccgtccga gacggtgagg
     8581 gccagctccg accggtcggc gaagaacgga gtgagctcca tgtggcactt ttccacgggc
     8641 gtcaggacca ggggatgcgt ggcagggtac agcccccgat ttttcagcga ttgtcccagg
     8701 taacggacga ccccggcgat cgcgctctcc atctgtcttc ggtccacgtt gtctcccctc
     8761 tcttacggta cgcgtcttgc gatgattcgc tcggcctcga tccggagcga ccggtcggcg
     8821 gaacgccggg cccgctcgag aacctccttc gaccgctctc ccttgatctt tgagatgctc
     8881 cggagggcgt ggaatttcat cgcccggaac tttttcgtgg accacagggc gcgcagggcg
     8941 aggatctctt cgagggcggt gaccgcctcg tcggtgccga tcgtccggag ggagtcgatc
     9001 gcctcgtgag ccagcctgta gttcgggtag aggaagagtt tggccgccgc ccggcggtac
     9061 agggcgacga ccgcctccgt ttcctttttc gatgacaggg cggcggtggc ggtgagagcc
     9121 accgtttcct cggggaaaaa ggagagatcg cagagggtgg cgacggcgga agggtggggg
     9181 atcctcgaga gcgccttgat cgcctccttc ttcacacgca ggtcgggatg gggaagcgtg
     9241 gcggcgacgt gcggcgcgag gtcgggcatc ccgagggttc cgaggacggt gatcatgttt
     9301 cgcaccatgt accagcggga gtccgtcagg ttctccagga tcgtggggac cgcgatcctc
     9361 ccgatccgcg tcacgatctc gacgatcgcc ttccggacga gcaggtcctc ctcctcggcc
     9421 agggcctgga gcagcggccc gacggagcgc tcttccagcg cgacgagcac ggcatcggtc
     9481 tcctcgcggc cacgggctcc gcgcttcttg agcaggccga tgtaatgcgc caccagttct
     9541 tcggaggcca cctccttgat cccgaggcgg gcgagtccgg cgatctcgtc gcttcccccg
     9601 ggaggaaact ctatgtgccg gatgaagatc gtcatcgcct gcgtcgagaa ttcgatcttc
     9661 cgctcggcgc gttccgcgag gaggaaccg
//
LOCUS       DASVJL010000246         2850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1165027, whole genome shotgun sequence.
ACCESSION   DASVJL010000246 DASVJL010000000
VERSION     DASVJL010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165027"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1793)
                     /locus_tag="VF357_05040"
     CDS             complement(213..1793)
                     /locus_tag="VF357_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7523543.1"
                     /translation="MKSQRPPVSIPNEPLTFPLRRAAREYPDKIAVITPEAERKEYSY
                     RWLEEASSRLAASLAGLGVSPGDRVGLWLKNSVEYILSFYGILKAGGIIVPMSTHYGE
                     REVLHQLEVTGAIGIVSSDDRYAQAAGLSEARAGLRFRVADGEGAAPPGTVRFAGLLR
                     GNDLLDRSMGIDPAETLAVLPFSSGTTGHPKGVMLTHTNLLSNLHQIRQAHEVREGDL
                     FMNQLPFFHIYGMTVLMGCSILAGATQVLASRFRPVDEFLALFERYRPTLFLTVPLIL
                     QEFCHHPKVPGMDWSRMRYVNTGGAPMSPELQERFRQLTGVTVIQGYGLTESSPTTHV
                     VPLDRIRVGTIGTPISLTEDRIADPETGRELPPGEVGELWVRGPQVMKGYYQDPEATA
                     RTLVDGWLRTGDLARRDEEGYVVIVDRLKELIKTNGFQVAPAEIEHILVGHPDVRDAA
                     VIGVQHPDHGEVPVAFVVRKEGAAVTGEELIDFAAKGLAKYKRLSRVMFVGAIPRSAS
                     GKILRRVLKESPPVLGQV"
     gene            complement(1790..2779)
                     /locus_tag="VF357_05045"
     CDS             complement(1790..2779)
                     /locus_tag="VF357_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7523544.1"
                     /translation="MGRPRGKAIPEYVAGAVVFAAVTVLPFALSFNPYYLSIFVAALI
                     LGSVSMAWNLLAGVCGQVSFGHAAFFGVGAYASAILVMKAGWNPYLAMVAAGGIGVGA
                     ALLIGLPAFRLRGPYFALAILGFAEVMKLLALNLTGLTEGSQGIFNIPPPPPIHLGGM
                     TIDFYVSRLTNYYFAALLMLGVYLSVYFLRRSDTGLAMFAVHGDEETAAGIGVPVVRT
                     KALALSVSAFFTALMGAFYAHYVHFLSPDSAFDGAWSVMPIVGSLFGGMGTLIGPSVG
                     ALVITGLDEFVFKKFFETGHKLFFGLLLAVVIVWAPSGLLGKRVGKSSLGGKG"
BASE COUNT          543 a          932 c          939 g          436 t
ORIGIN      
        1 gaagggctgg cgacgttccg ttgtcagccc tttttcattg ccttggtgct caagagggac
       61 ttgaacgggc gaggatcgcc gagcgattag cgaggaagag cgcgacgcac gggagcgcgg
      121 aagatccgag ccccgccgca aaggccgatg gcctggctgg ccgaggctca ggcgggcaag
      181 tccctcctcc ggcaccaatt aaaaacgcgc agttacacct gcccgagtac cggcgggctc
      241 tccttcaaaa ccctccggag gatcttgccg gaggcgctgc gcgggattgc ccccacgaac
      301 atcacccggg agaggcgctt gtacttcgcc aaccctttcg ccgcgaaatc gatcagttcc
      361 tcccctgtga cggccgctcc ctccttgcgt accacaaaag ccacgggaac ctcgccgtga
      421 tcggggtgct gcaccccgat gaccgccgca tcgcggacgt cggggtgccc tacgaggatg
      481 tgctcgatct ccgcaggcgc cacctggaat ccgttcgttt tgatgagctc ttttaggcgg
      541 tcgacgatga cgacatatcc ctcctcgtcc ctgcgggcca ggtcgccggt gcgcagccag
      601 ccgtccacca gcgttcgggc cgtcgcttcc gggtcttgat aatacccctt catcacctgg
      661 gggccgcgga cccagagctc ccccacctct cccggcggca gctcccgccc cgtctccggg
      721 tcggcgatcc ggtcctccgt aagcgagatc ggcgtcccga tggtccctac cctaatccgg
      781 tcgaggggga ctacatgcgt cgtgggggaa gactccgtca ggccgtaccc ctggatgacg
      841 gtaacgccgg tgagctgccg gaaccgctcc tggagctccg gggacatcgg cgccccgccc
      901 gtgttcacgt accgcattcg actccagtcc atcccgggga ctttcgggtg gtggcagaac
      961 tcctgcagga tcagggggac agtgaggaac agggtcggcc gatatctctc gaaaagggcg
     1021 agaaactcgt ccaccggccg gaagcggctc gccagcacct gtgtcgcccc cgccaggatg
     1081 gagcacccca taaggacggt catgccgtag atgtggaaga aggggagctg gttcatgaag
     1141 aggtcccctt cccgcacttc gtgggcctgg cggatctggt gcaggttgct caggagattg
     1201 gtgtgcgtca gcatcacccc cttcggatga ccggtcgtcc cgctggagaa cgggaggacg
     1261 gccaaggttt ccgcgggatc gatccccatg gagcgatcca ggaggtcgtt tccccggagc
     1321 aaccccgcga accggaccgt tcccggcggg gccgccccct cgccgtcggc gacgcggaac
     1381 cgcagccccg cgcgggcttc ggagagaccg gccgcctggg cgtaccggtc gtcggagctc
     1441 acgattccga tcgccccggt gacttcgagt tggtgaagga cctcccgctc gccgtaatgg
     1501 gtgctcatcg gaacgatgat cccgcccgcc ttgaggatcc cgtagaagga gaggatgtac
     1561 tcgacgctgt tcttcagcca cagaccgacc cggtccccgg gggaaactcc cagcccggcg
     1621 agggaagccg ccagccggga cgacgcctcc tcgagccacc ggtacgagta ctccttcctc
     1681 tccgcctccg gggtgatcac ggcgatcttg tcggggtatt cccgcgcggc ccgtcgcagg
     1741 gggaaggtga ggggctcgtt cgggatcgat acgggggggc gttgtgactt catcctttcc
     1801 ctccaagcga agatttccct acccgctttc cgagcaggcc cgagggggcc cagacgatca
     1861 ccaccgccag gaggagaccg aagaagagct tgtgccccgt ctcgaagaac ttcttgaaga
     1921 cgaactcgtc cagaccggtg atgacgaggg caccgacgga gggaccgatg agcgtcccca
     1981 tccccccgaa gagagagccc acgatcggca tcacggacca tgcgccgtcg aacgccgagt
     2041 cgggagacag aaagtgaacg tagtgggcgt agaaggcccc catcagcgcc gtgaagaagg
     2101 ccgagacgga gagtgcgagc gccttcgtcc gaaccacggg caccccgatc cccgcagcgg
     2161 tttcctcgtc cccatggacg gcgaacatcg ccaggccggt gtcggagcgg cgcaagaaat
     2221 agacggaaag gtagacgccg agcatcagaa gggccgcgaa gtagtagttc gtgagccgcg
     2281 agacgtagaa atcgatcgtc atccccccca ggtggatcgg cggaggcgga gggatgttga
     2341 agatcccctg ggacccttcc gtcagccccg tgaggttgag cgcaaggagt ttcatcacct
     2401 cggcgaatcc aaggatcgcc aaggcgaagt acgggccgcg gagacggaag gcggggaggc
     2461 cgatcaggag agccgcgccg acgccgattc cccctgcggc gaccatcgcc aggtagggat
     2521 tccagccggc cttcatgacg aggatggccg aggcgtacgc gccgaccccg aaaaaggcgg
     2581 cgtggccgaa cgacacctgg ccgcagacgc ccgccagcag gttccacgcc atggagacgg
     2641 atccgaggat cagagcggcg acgaaaatgc tcaggtagta cgggttgaac gacagcgcga
     2701 agggtaggac cgtcaccgcg gcgaagacca cggcgccggc gacgtattcg gggatggcct
     2761 tcccgcgggg ccggcccacg ctcagatcct ccttccgaac agcccggaag gccgaaggag
     2821 gaggatcccc aggaacagga ggaatccgaa
//
LOCUS       DASVJL010000247         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_413830, whole genome shotgun sequence.
ACCESSION   DASVJL010000247 DASVJL010000000
VERSION     DASVJL010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413830"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1196
                     /locus_tag="VF357_05050"
     CDS             219..1196
                     /locus_tag="VF357_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX7523545.1"
                     /translation="MKQGREKLVRLYRLMFLTRRTEETANQLFLEGKIRTMGHWSTGQ
                     EAAGVGVGAALRSDDCLFPTHRAWPEFIGKGMSPGSIVAEFIGKGTGCAKGKGGLHIS
                     SAEHGIVGLVGSLGSDFPMAVGAALSAKMRRTDQVSCVYFGEGTATQADFHPAMQMAM
                     LWNLPVIFACVNNQYVELVHYREVICFENMLDLAQGYHMNAIPVPDGNDVAAVFDAMS
                     EAVARARRGEGPSMLEIKTYRVGTHFTGDAGAYMPQEEVAAWKARDPIARCRARLLAE
                     RALNEGELLSLEKEVEAEVREAIRFAMESPHPSVEEAFSDVYVTREVVR"
     gene            1193..2164
                     /locus_tag="VF357_05055"
     CDS             1193..2164
                     /locus_tag="VF357_05055"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX7523546.1"
                     /translation="MSRKILYVQAIGEAFAEEMARDERVFIMGIDVTRAVFGMTAGLV
                     DRFGKERVRNTPICESGIVGTALGAALTGMRPCAEIMFSDFTYVAMDQIATQMGSWRY
                     MTGGQFKAPMVVYTLDGGGMGLGYNHSGCTESVFQGSPGLTVVAASDAYMAKGLLKAA
                     IRSDDPVLFFGHKALLGQSGEVPDEDYILPLTKARIVREGGDVTVVAHHLMLHHALGV
                     AAALEKEGVSVEVIDPVCISPLDRETILASVRKTGRLVTVEEGRRAGGVGAEISAVVS
                     EEAFFDLDAPIKRVGAPPIPVPGSAFLEQSCYLPGPEDIRKAITSIL"
     gene            2208..>2988
                     /locus_tag="VF357_05060"
     CDS             2208..>2988
                     /locus_tag="VF357_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7523547.1"
                     /translation="MVTGVYVPRWGVTMEQATIAAWLVKEDEPVVKGQDLVELETEKI
                     VNVVQAPADGVLRRILVPKGETAPVGALLGVISEAGEAFDLEALRGADAQVQTAGPGE
                     RLSRTGRPAEKNQRGRVRASPPARKAAEELGLDLAVLEGTGPEGSVSRDDVERAIAAA
                     VARSLDEGDKTVEGVSLHYVSVGGEKTGLKTSPTPVVLVHGIAGSTLLWQANLSAIGA
                     GHKVFAFDLPGHGRSGKSAGPCSIDLFTRSLLGILDACGLEK"
BASE COUNT          542 a          888 c         1082 g          476 t
ORIGIN      
        1 gaaaacggat caacacggag gtgatgatct ccgacaccat cgatctggcg gacattgaag
       61 agagagggtt ccggcggctc ctcgcctccc gggatgacgt gaagatcgtg gtgaagcctt
      121 aaggtggcat cgcccctgac ctgaccgccg gggccggacc aaactacaac aacggtagac
      181 cccggaaccc gcgggggacc acggaaagga tggcatcgat gaagcaaggg agagaaaagc
      241 tcgttcgcct gtaccgcctc atgttcctca cccggcggac ggaagagacg gcgaaccaac
      301 tgttcctgga ggggaagatc cgaacgatgg gccactggtc cacggggcag gaggcggccg
      361 gggttggggt tggggcggcc ttgcggtccg atgactgtct tttccccacc caccgtgcgt
      421 ggccggaatt catcgggaag gggatgtcgc ccggaagcat cgtcgcggag ttcatcggaa
      481 agggaacggg gtgcgccaag gggaaggggg ggctccacat atcgagcgcg gaacacggga
      541 tcgtggggct ggtgggaagc ctcggctccg atttccccat ggccgtgggg gctgccctct
      601 ccgccaagat gcggcgaacc gaccaggtct cctgcgtcta cttcggagag ggaacggcca
      661 cccaggccga tttccacccg gccatgcaga tggcgatgct ctggaacctg ccggtgatct
      721 tcgcgtgcgt gaacaaccag tatgtggagc ttgtccacta ccgcgaggtc atctgtttcg
      781 aaaacatgct cgacctggca cagggctacc acatgaacgc gatcccggtg ccggacggca
      841 acgacgtggc ggcggttttc gatgccatga gcgaagcggt cgcccgggcc cggcgggggg
      901 aggggccgag catgctggag atcaagacgt accgcgtcgg gacccacttc accggggatg
      961 cgggcgccta catgcctcag gaggaagttg ccgcctggaa ggcccgggac ccgatcgcgc
     1021 ggtgccgtgc caggctcctg gcggaaagag ccctgaacga gggagaactc ctctccctgg
     1081 agaaggaagt cgaggcggag gtgcgggaag ccatccggtt cgcaatggaa agcccccatc
     1141 cctcggtgga agaggcgttc tccgatgtct atgtgacccg ggaggtggtg cgatgagccg
     1201 caagattctt tatgtccagg cgatcggcga ggcgtttgcg gaggagatgg ccagagacga
     1261 gcgggtcttt atcatgggca tcgatgtcac gcgggcggtc ttcgggatga cggcgggcct
     1321 ggtggatcgt ttcggcaagg agcgggtccg gaacaccccg atctgcgagt ccgggatcgt
     1381 cgggacggcc ctgggcgcgg cgctgacggg gatgaggccg tgcgccgaga tcatgttcag
     1441 cgatttcacg tacgtggcca tggaccagat cgccacccag atggggagct ggcggtacat
     1501 gacgggaggc cagttcaagg ctcccatggt cgtctacacc ctggacggcg gggggatggg
     1561 tctcgggtac aaccactccg gttgcaccga atcggtgttc cagggcagcc ccggactcac
     1621 cgtcgtggcg gcctccgacg cttacatggc aaaggggctt ttgaaggcgg cgatccgcag
     1681 cgacgacccg gtgctcttct tcggccacaa ggcgctgttg ggccaatccg gggaggttcc
     1741 ggacgaggac tacattttgc cgcttaccaa ggcccggatc gtccgggagg gaggcgacgt
     1801 cacggtcgtt gcgcatcatc tgatgctcca ccatgccctc ggcgtcgcgg cggccctgga
     1861 gaaagagggc gtgtcggtgg aggtgatcga cccggtctgc atctccccac tcgaccggga
     1921 gacgatcctc gcctcggttc gaaagaccgg gcggctcgtc acggtcgaag aggggcggcg
     1981 ggcgggcggc gtcggcgccg aaatatccgc ggtggtcagc gaggaggcgt tcttcgacct
     2041 ggacgcgccc atcaagcgcg tgggagcgcc acccatcccg gtgccgggga gcgctttcct
     2101 ggaacagagc tgttacctgc ccggccccga agatatacgg aaggcgatca cctcgatcct
     2161 gtgaggttac cctgcccggt gcatgggcca aacaaggagc gttgaagatg gttaccggcg
     2221 tgtacgtgcc ccgctggggc gtaacgatgg agcaggccac gattgccgct tggctcgtga
     2281 aggaagacga gccggtcgtc aaaggccagg acctggtgga gttggagacc gagaagatcg
     2341 tgaacgtcgt gcaggccccg gcggacgggg tcctgcgccg tatcctcgtc ccgaaagggg
     2401 aaaccgctcc ggtgggagct ctcctcggcg tgatctcgga ggcgggcgag gcgttcgatc
     2461 tggaggccct tcgaggcgcg gacgcgcagg tccaaacggc aggccccggg gagcggcttt
     2521 cgcggacggg ccgccccgcg gagaagaatc agcggggtcg ggtccgggca tcaccgccgg
     2581 cccggaaggc tgccgaggaa ctcggcctcg acctcgcggt tctggaaggc acgggaccgg
     2641 aggggagcgt gagccgcgac gacgtggagc gcgcgatcgc ggcggccgtg gcgcggagcc
     2701 tggacgaagg cgacaagacc gtcgaggggg tgtccctgca ttacgtgtcc gtcggaggcg
     2761 aaaagaccgg tctcaagacg tcgcccaccc ccgtcgtcct cgtccacggc atcgcgggca
     2821 gcaccctcct ctggcaagcc aacctgtcgg ccatcggcgc gggacacaag gtgttcgctt
     2881 tcgacctgcc gggtcacggg cgctccggaa agtctgcggg tccctgcagc atcgatctct
     2941 tcacgaggtc gctcctgggc atcctggacg cctgcggact cgagaagg
//
LOCUS       DASVJL010000248         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1165134, whole genome shotgun sequence.
ACCESSION   DASVJL010000248 DASVJL010000000
VERSION     DASVJL010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165134"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /gene="rplS"
                     /locus_tag="VF357_05065"
     CDS             complement(<1..297)
                     /gene="rplS"
                     /locus_tag="VF357_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX7523548.1"
                     /translation="MNLLKDIESRQQRKDLPKFSVGDTVRVYSRIKEGEKERVQYFEG
                     IVIGFHRNAVSTTFKVRKESYGVGVELTYPIHSPLLEKIEVKKRGDVRRAKLFYL"
     gene            complement(373..1065)
                     /gene="trmD"
                     /locus_tag="VF357_05070"
     CDS             complement(373..1065)
                     /gene="trmD"
                     /locus_tag="VF357_05070"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX7523549.1"
                     /translation="MRIDVLTLFPGMFPGPLSHSILGRAREAGLLAVEVHDLRSYAEG
                     KHRVTDEPPFGGGGGMVMKPEPIFAGVEALRAAHGPGRVLLLSPAGSLLTPEVAARLA
                     KEDHLVLLCGRYEGVDQRVADHLADEELSIGDYVLSGGELPALVLIDAVARFLPGVLG
                     DPAAPHNDSFSHGILEAPHYTRPRDFRGWTVPDVLLSGDHAAVAAWRKAEGERRTGRN
                     RPDLLKKKLTDP"
     gene            complement(1067..1573)
                     /gene="rimM"
                     /locus_tag="VF357_05075"
     CDS             complement(1067..1573)
                     /gene="rimM"
                     /locus_tag="VF357_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX7523550.1"
                     /translation="MDIGRVTGLHGVRGKVKVAAFSGDPEGVLAAKTLRLSGGRGTAS
                     GAVGDYEVVTAQRAGGCAVFSLKGIETIEAAEPLVKAVVSVRYDELPSLPEDEFYWID
                     AVGCLVVDETGAPLGEVVAVEPGAAYDYLVVRRPGGEDAYLPVVAAFLRKVDTAARRV
                     VASPPEGW"
     gene            complement(1606..1890)
                     /gene="rpsP"
                     /locus_tag="VF357_05080"
     CDS             complement(1606..1890)
                     /gene="rpsP"
                     /locus_tag="VF357_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX7523551.1"
                     /translation="MAVKIRLARTGRKKMAFYRVVVADSKMPRDGRCIAYVGTYAPRE
                     NPAKIVIDEAVTLKWLSKGALPTETVKSLLKKSGAWKKFQETKASKPATA"
     gene            complement(1942..>2952)
                     /gene="ffh"
                     /locus_tag="VF357_05085"
     CDS             complement(1942..>2952)
                     /gene="ffh"
                     /locus_tag="VF357_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX7523552.1"
                     /translation="GSGKTTTCGKLALYLQKKKRTPFLVPADVYRPAAIEQLKVLGRQ
                     LEIPVFDSRPDADPVEICREALKYAELNGYDTVLLDTAGRLHIDAPLMEELSRIKSAV
                     DPGDILLVADAMTGQDAVNVAKAFHEKLTLTGVVLTKADGDARGGAALSIRAVTGAPV
                     KFVGTGEKLDALEPFHPERMASRILGMGDILTFVEKAQDQVDEKQAKELERKLRKNEF
                     TLEDFRDQLLRIKKMGSMEEILGMIPGLGAKAKELKGMAPDETELKRVVAIIDSMTAQ
                     ERRNARILNGSRRKRIAAGSGTTVQDVNRLMKNFQQAEAMIQRMTKGGMRGMGRNLPF
                     SR"
BASE COUNT          459 a         1055 c          884 g          554 t
ORIGIN      
        1 caggtagaag agcttcgcgc ggcggacatc gcccctcttc ttcacctcga tcttttcgag
       61 gaggggcgag tggatcgggt aggtgagctc cacgccgacg ccgtacgact ccttgcggac
      121 cttgaaggtg gtggagacgg cgttccgatg gaagccgatc acgatcccct cgaaatactg
      181 gacgcgctcc ttctcgcctt ccttgattcg cgagtagacc cggacggtgt cccccacgct
      241 gaatttcggg agatccttgc gctgctgccg ggattcgata tccttgagga gattcatggc
      301 cctatcctgt cctttccttg catgcggttg cgggtgatga gatgaagaag acctgacaat
      361 atattccaac cgctatgggt ctgtcaattt tttcttcagc aggtccgggc ggttccgccc
      421 cgtccggcgc tccccttccg ccttgcgcca cgccgcgacg gcggcatggt cccccgacag
      481 gagaacgtcg gggacggtcc atccccggaa gtcgcgcggc cgcgtgtagt ggggcgcttc
      541 caggatcccg tgcgaaaacg agtcgttgtg cggcgccgcc gggtccccca gcacccccgg
      601 caggaaccgg gccacggcgt cgatcagcac cagcgccggc agttccccgc cggagaggac
      661 ataatccccg atggagagct cttcgtcggc caagtggtcc gccacccgct ggtccacccc
      721 ttcgtaccgg ccgcagagga ggaccagatg atcctccttc gccagccgcg cggcgacctc
      781 cggcgtcagg agggagcccg cgggggaaag gaggaggacc ctccccggcc cgtgggccgc
      841 gcggagcgcc tcgaccccgg cgaagatcgg ctcgggtttc atcaccatcc cgccgccgcc
      901 gccgaagggg ggctcgtcgg tcacccgatg cttcccctcc gcataggaac gcaggtcgtg
      961 cacttccacc gcgagaagcc ccgcttccct cgcccgtccg aggatgctgt gggaaagcgg
     1021 ccccggaaac atcccgggga aaagcgtcag gacgtcgatc cgcacgtcac cacccctcgg
     1081 gcggagaggc gaccacccgc cgcgccgccg tgtcgacctt gcgaagaaag gctgccacca
     1141 cggggaggta cgcgtcctcc cctcccggcc gccggacgac caggtagtcg taggctgccc
     1201 ccggctccac cgccaccacc tcgcccaaag gagcgcccgt ctcgtccacc acgaggcagc
     1261 cgactgcatc gatccagtag aattcgtctt cggggaggga ggggagctcg tcgtaccgca
     1321 cggagacgac cgccttcaca agcggttccg ccgcctcgat ggtttcgatc cccttcaacg
     1381 agaaaacggc gcagccgccc gcgcgctgcg ccgtcaccac ttcgtagtcg ccgaccgccc
     1441 cggacgccgt cccgcggcct ccggagagcc gcaacgtctt ggcggccagg accccctcgg
     1501 gatcccccga gaacgccgcc accttgacct tgccgcgaac gccgtggagg ccggttacac
     1561 ggccgatatc caataatcct tcccggccag gaaggcccct catggtcaag ccgtggcggg
     1621 tttgctcgcc ttggtctcct ggaacttttt ccaggcgccg gactttttca gaaggctctt
     1681 gaccgtttcg gtcggcagcg cgcccttcga gagccatttg agggtcaccg cctcgtcgat
     1741 cacgatcttc gccgggttct cccggggagc gtacgtcccg acgtacgcga tgcagcgccc
     1801 gtcgcggggc atcttcgagt ccgccaccac cacgcggtag aaagccatct tttttcggcc
     1861 tgtccgcgcc aaacgaatct ttaccgccat gaaccgatcc tcctgctttt cattggaagt
     1921 ctcgctactt taccacattc cctaccggga aaagggaagg ttcctcccca tgccgcgcat
     1981 cccgcccttc gtcatccgct ggatcatcgc ctccgcctgc tggaaattct tcatcaggcg
     2041 gttcacgtcc tgcacggtcg ttccgctccc cgcggcgatc cgcttccggc ggctgccgtt
     2101 gaggatccgc gcgtttcgcc gctcctgcgc cgtcatcgag tcgatgatgg cgacgacccg
     2161 cttcaactcc gtctcgtcgg gcgccatccc tttcagctcc ttcgccttcg cgccaagacc
     2221 ggggatcatc ccgaggatct cttccatcga ccccattttt ttgatccgca gcagctggtc
     2281 ccggaaatcc tcgagcgtga actcgttctt ccggagcttc cgctcgagtt ccttcgcctg
     2341 tttttcgtcg acttggtcct gcgccttctc gacgaaggtg aggatgtcgc ccatccccag
     2401 gatacgggag gccatcctct ccgggtggaa cggttcgagg gcgtcgagct tctcccccgt
     2461 tccgacgaac ttcaccgggg ctccggtcac cgcacggatc gagagggccg ccccgccgcg
     2521 ggcgtcgccg tccgccttcg tcagcaccac gccggtaagc gtcagctttt cgtggaacgc
     2581 cttcgccacg ttcacggcgt cctgcccggt catcgcgtcg gccaccagga ggatgtcccc
     2641 cggatcgacc gccgacttga tccgggagag ttcctccatc agcggcgcgt cgatgtgaag
     2701 gcgccccgcc gtatcgagga ggaccgtgtc gtatccgttc agttcggcgt atttcaatgc
     2761 ctccctgcag atctccaccg ggtcggcgtc ggggcgggag tcgaacaccg ggatctccag
     2821 ctgccgcccg aggaccttca gctgctcgat ggcggccggg cggtagacgt cggcggggac
     2881 caggaacggg gtccgcttct tcttctggag atacagggcg agtttgccgc acgtggtcgt
     2941 cttccccgat cc
//
LOCUS       DASVJL010000249         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1446162, whole genome shotgun sequence.
ACCESSION   DASVJL010000249 DASVJL010000000
VERSION     DASVJL010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446162"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VF357_05090"
     CDS             <1..835
                     /locus_tag="VF357_05090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX7523553.1"
                     /translation="RGASGILCTLADRVDAELMDAAGPSLVVVSNFAVGVNNVDVAGA
                     TRRGIRICNTPDVLTDATADLGFALLLSAARKVSDADRFVRAGGWTGWDPWGFLGVPV
                     AGKTLGIVGMGKIGAAVARRSRGFSMKVLYHNRRRIPPSEEAGLGASYRDLDALLAES
                     DFVVLCVPLTPETRGLISTERLGKMKRTAVLVNISRGDVVDEEAVAAALAEGRLFGAG
                     LDVFEKEPRVHPRMLSAPSAVLLPHLGSATGETREAMGRLATENLLAVLDGREPPCPV
                     N"
     gene            complement(828..1343)
                     /locus_tag="VF357_05095"
     CDS             complement(828..1343)
                     /locus_tag="VF357_05095"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEX7523554.1"
                     /translation="MRIVKRWVLLLLFGALCLPVSSCRPLLKEVFKAPKVRLVDIGVA
                     GNPFVSRGPVEAILHLAVNNPNSYALTVAGVAYSATVGTRQVADGERTEEIRIEPSGE
                     TVVKVPVRLQTDVFVAALREVLDARSVSYEFTGSVSVVAPVVGVVRVPFSRTGTIDPM
                     DIFRRKGIGFN"
     gene            1330..>1538
                     /locus_tag="VF357_05100"
     CDS             1330..>1538
                     /locus_tag="VF357_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523555.1"
                     /translation="MTIRIFDVRVDPSLPERSLPARIAMIIGVPIDEVQRFSVVRRGY
                     DARRKGDVRRVYTVECSLGSPAMEEA"
BASE COUNT          232 a          505 c          569 g          232 t
ORIGIN      
        1 ccggggggcg tcggggatcc tctgcacgct ggcggaccgg gtggacgcgg aactgatgga
       61 cgcggccggg ccctcccttg tcgtagtgtc gaacttcgcc gtcggggtga acaacgtcga
      121 cgtggccggg gcgacccggc gcgggatccg gatctgcaac acgccggacg tcctgacgga
      181 cgcgacggcc gacctcgggt tcgccctgct cctttccgcg gccagaaagg tgtcggacgc
      241 cgaccggttc gttcgcgcgg ggggctggac cgggtgggac ccgtgggggt tcctcggtgt
      301 ccccgtcgcc ggaaagacgc tggggatcgt cggaatgggg aagatcggcg ccgcggtggc
      361 ccgccggtcc cgcggcttct ccatgaaggt cctctaccac aaccgtcgcc ggattcctcc
      421 gtcggaggag gccggactcg gggcgtcgta tcgcgatctc gacgcgctgc tcgccgagag
      481 cgacttcgtc gtactgtgcg tccccctcac cccggagacc cgcgggctga tctccacgga
      541 gcgcctcgga aagatgaaac ggacggcggt cctggtgaac atctcccggg gagatgtggt
      601 ggacgaggag gcggtggcgg cggcgctggc ggaggggcgg ctgttcggcg cggggctcga
      661 cgtgttcgag aaggagccgc gggttcaccc gcggatgctg tcggcccctt cggcggtgct
      721 cctgccgcac cttgggagcg cgaccgggga gacgcgggag gcgatgggtc ggctggcgac
      781 ggagaacctg ctcgccgtgc tcgacgggcg agagccgccg tgccccgtca attgaagccg
      841 atccccttcc ggcggaagat gtccatgggg tcgatcgtcc cggtccggga gaaggggacc
      901 cggaccacgc cgacgaccgg ggcgaccacg ctcaccgaac cggtgaactc gtaggagacc
      961 gaccgggcat cgagcacctc gcgcagcgcg gcgacgaaga cgtcggtctg cagccgcacg
     1021 ggcaccttga ccaccgtttc cccggagggc tcgatgcgaa tctcctcggt ccgctccccg
     1081 tcggccactt gccgggttcc caccgtcgcg gaataggcga cgccggccac cgtcagggcg
     1141 taggaattcg ggttgttcac cgcgagatga aggatcgcct cgaccgggcc ccgggacacg
     1201 aacgggttgc cggccacccc gatgtcgacg agccgcacct tcggggcctt gaagacctct
     1261 ttcaggaggg ggcggcacga ggagacggga aggcacaacg ctccgaacag caggaggagg
     1321 acccaccgct tgacgatccg catcttcgac gtccgtgtcg atccgtcgct tccggaacga
     1381 tctctgcccg cgcgcatagc gatgattata ggcgttccga tcgacgaggt gcagcggttt
     1441 tccgtggtcc ggcgggggta cgacgcccgc cggaaaggcg acgtgcgccg cgtctacacg
     1501 gtggagtgct ccctcggctc ccccgcgatg gaggaggc
//
LOCUS       DASVJL010000250         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_133419, whole genome shotgun sequence.
ACCESSION   DASVJL010000250 DASVJL010000000
VERSION     DASVJL010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133419"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VF357_05105"
     CDS             <1..337
                     /locus_tag="VF357_05105"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX7523556.1"
                     /translation="SLAGRKVVLSKEGYERQSVPADALAVGPAFRAELQPLIGTVEAI
                     QAIPWAKVYLGNRLLGETPLTAVRLPAGEQRLRFVNEPLGVDQVRIIVVRPGANPKVI
                     VPITGAGGR"
     gene            complement(303..569)
                     /locus_tag="VF357_05110"
     CDS             complement(303..569)
                     /locus_tag="VF357_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523557.1"
                     /translation="MERRYGTWIWAVLITVPLLLCVAAPGFVEAGSLRRGGPALESGG
                     GKSIRLSLKISDAKKRKREVSFYKSVARQAKIRPTGLPPPLSGR"
     gene            complement(575..1393)
                     /locus_tag="VF357_05115"
     CDS             complement(575..1393)
                     /locus_tag="VF357_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7523558.1"
                     /translation="MRARDFFLIGAPMLLGGSILVALLQRAWKGRSIACAMQAYRSAH
                     DRLTEGLVAKRLLRFPGVCLVSSLRHWAFAELFALLAFLAIVSADLSASGLAQATAAA
                     ALLGSGIAWISLRGAVRETLRSVQRDLPVACFLFSLLLESGMGASSALQETSGSIPEG
                     ALARELGELVRSRSLGVPRGESIERSRRRVPVEDYRLFLSHVLQGERLGIGLSRSLRE
                     LSTKMLENQGHRAETIAQEAAVKMLFPLVVFIFPAVFLIILSPVILGLWDRIAG"
     gene            complement(1390..2241)
                     /locus_tag="VF357_05120"
     CDS             complement(1390..2241)
                     /locus_tag="VF357_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7523559.1"
                     /translation="MGLTLAEAGIFVLGAALIARFLLRAALPQARTRFASAATRHSND
                     LREVFVHLPPSRIAAALLASATVLAGASLAATRSVAVAAASGTAPVLFAGLLIRWYRD
                     RRKRSILFQLPTLLDLLSGHVRAGHSLSESLAETVPLLPAGIREEMAWVLQQNRLGTT
                     LPEALAHWEERIRSEETSLFVRPLRAAIPGGGNIVDLLERTRDILRLRMRTTEKLRSM
                     TAQARLQALVLTLLPPAFAVALSKVDPGFFPNLLGTPQGKAIMAIAFSLQVLGWVTIR
                     KILSVRP"
     gene            complement(2232..3455)
                     /locus_tag="VF357_05125"
     CDS             complement(2232..3455)
                     /locus_tag="VF357_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX7523560.1"
                     /translation="MNGDIRRTLHQAVLSRLDARKTGLSFSEDVGRWRDRAEEYLQAE
                     MSGLSLGDAERDALRREILDEIFAFGPITPLLSDPAVSEIMVNGYESIYVERGGLVAR
                     HGGSFLSEESLRATIDRMVSKVNRRLDESSPYVDARLPDGSRINAIIPPVCLTGACLT
                     IRKFRKEAFSLEELVRIGSVAQDAADYLGEAVRERRNVIVSGGTGSGKTTLLNALSQF
                     IPEEERIVTIEDAAEIRLQKPHVIRLEAKPANIEGSGAVTIRDLVRNSLRMRPDRIIV
                     GECRGGEALDMLQAMNTGHDGSITTGHANTPRDMLRRLETMVLLCGVEIPIRAVREQI
                     ASAIDIIVHAGRIAGGKRAVTSITEITGMNESQILLQELFRWSKGNAGNDGAGRLVAT
                     GTPSRFLRGGDDTWD"
BASE COUNT          649 a         1208 c         1206 g          588 t
ORIGIN      
        1 ctccctcgcg ggccggaagg tcgtcctttc gaaggagggg tacgaacgac aatccgttcc
       61 ggcggacgcg ttggcggtgg gaccggcatt ccgcgccgaa ctgcagcccc tgatcgggac
      121 ggtggaggcg atccaggcga tcccgtgggc gaaagtctac ctcgggaacc gtctgctcgg
      181 ggagaccccg ctgaccgccg tgcgcctgcc ggcgggagag cagcggctcc gcttcgtgaa
      241 cgagccgctt ggggtcgatc aggtgaggat catcgtcgtc cgtccgggcg caaacccgaa
      301 agtcatcgtc ccgataacgg gggcgggagg ccggtaggcc ggatcttcgc ctgccgcgcc
      361 acgctcttgt agaaggaaac ctcccgtttc ctctttttcg cgtcggagat cttgagcgac
      421 agccggatgg attttccgcc tccggattcg agggcaggcc cccctctccg cagggatccc
      481 gcttccacga atccaggtgc cgccacgcac agaaggagcg gcacggtgat cagcaccgcc
      541 catatccatg tcccatatct gcgttccatg aggttcaccc cgcgattctg tcccacaggc
      601 caaggatcac cggggacagg atgatgagaa acacggcggg aaagatgaaa acgaccaaag
      661 ggaacagcat cttcaccgcc gcctcctggg cgatcgtctc cgcccggtgc ccctggtttt
      721 ccagcatctt cgtggagagt tcccgcaggc tccgcgacag tccgatcccg agccgctccc
      781 cctggagtac atggctgagg aacagacggt aatcctcgac gggcacacgc cgccgcgatc
      841 gctcgatcga ttctccccgg ggaacgccga gcgagcggga ccggaccaac tctcccagtt
      901 ccctggcgag ggccccctcg gggatcgagc ccgacgtttc ctgcagcgcc gaagaggcgc
      961 ccattcccga ttcgagcagg agggagaaga ggaagcacgc taccgggagg tcgcgctgca
     1021 ccgagcgaag cgtttcccgc acagctccgc gcaaggagat ccaggcgatg ccggagccca
     1081 ggagcgcggc ggcggccgtc gcttgcgcca acccggacgc ggaaaggtcg gccgaaacaa
     1141 tggcgaggaa ggcgagcagc gcgaagagct ccgcgaacgc ccagtgcctg agggaggaga
     1201 ccaggcaaac cccggggaat cgaaggagtc gtttcgcgac cagtccctcc gtcagccggt
     1261 catgggcgga ccggtacgcc tgcatggcgc acgcgatcga acgccccttc cacgcgcgct
     1321 gaaggagcgc gacgagaatg gagccgccca ggagcatcgg cgcgccgatg aggaagaagt
     1381 cccgcgccct catggccgca ccgacaggat ttttcgtatg gtgacccagc ccaggacctg
     1441 gagggagaac gcgatcgcca tgatcgcttt tccctgcggt gtgccgagga ggttcgggaa
     1501 gaacccggga tcgaccttcg aaagggcgac cgcgaatgcc ggtggcagga gcgtaaggac
     1561 caacgcctgc aatcgggcct gggccgtcat gctccgaagc ttttccgtcg tccgcatccg
     1621 gaggcgaagg atgtcccggg tgcgctcgag caggtccacg atgtttcccc ctcccgggat
     1681 cgccgcccgc aggggacgca cgaagaggga ggtctcttcg gaacggatgc gttcctccca
     1741 atgggccagg gcctccggaa gcgtcgtgcc caggcgattc tgctgcagga cccacgccat
     1801 ctcttcccga attccggcgg gcagcagggg aacggtttcc gcgagggact ccgacaagct
     1861 gtgccccgcc cgcacgtgcc cggacaaaag atcgaggagc gtcggcaact ggaagaggat
     1921 ggaccgtttt cgtcggtcgc ggtaccaccg gatcagaagg ccggcgaaga gaaccggcgc
     1981 ggtccccgat gccgccgcga ccgcaacgga ccgagtcgcc gcgagcgacg cccccgcgag
     2041 aacggtcgcg gaagcgagca gagccgcggc gatacgggac ggcggcagat ggacgaacac
     2101 ttcgcgcaag tcgttggaat gacgggttgc cgccgaggcg aacctcgtgc gcgcctgcgg
     2161 cagcgcggct cgaaggagaa accgggcgat cagagcggcg ccaaggacga agatccccgc
     2221 ctccgcgagc gtcagtccca cgtgtcgtct ccaccgcgga ggaaccggga aggggtgcct
     2281 gttgcgacaa ggcgccccgc accgtcgttt cccgcgttcc ccttcgacca tcggaacagt
     2341 tcctgaagca ggatctgcga ttcgttcatc ccggtgatct cggtgatcga cgtgaccgcc
     2401 ctttttccgc cggcgatccg cccggcgtga acgatgatgt cgatggcgga ggcgatctgc
     2461 tcccggaccg ctcggatcgg tatttccacc ccgcatagca ggaccatcgt ctccaggcgg
     2521 cgcaacatgt cccggggggt gttcgcgtgt cccgtggtga tcgagccgtc atggccggtg
     2581 ttcatcgcct gcagcatgtc gagcgcctcc cccccgcggc actccccgac gatgatccgg
     2641 tcggggcgca ttcgaaggga gttgcggacg aggtcccgga tggtgacggc ccccgaccct
     2701 tctatgttgg ccggcttcgc ctcgaggcgg atcacgtggg gcttctgaag acggatctcc
     2761 gccgcatcct cgatcgtgac gatccgctct tcctcgggga tgaactggga caatgcgttc
     2821 aggagggtgg tcttcccgga gccggtccca ccggagacga tgacgttgcg ccgttcccgg
     2881 acagcctctc ccaggtaatc cgcggcgtcc tgggccaccg acccgatgcg gacgagttcc
     2941 tcgagggaga acgcttcctt ccggaatttg cggatcgtca ggcacgcccc ggtgaggcaa
     3001 accggcggta tgatcgcgtt gatgcgtgac ccgtccggga gccgggcgtc gacgtagggg
     3061 gaggattcgt cgaggcgccg gttgaccttg gagaccatcc ggtcgatcgt cgcgcgaagc
     3121 gattcctccg agagaaacga gcctccgtgg cgggccacca acccgcctcg ctccacgtag
     3181 atcgactcgt atccgttcac catgatctcg gagacggcgg ggtcggagag caggggagtg
     3241 atcggcccga aggcgaagat ctcgtcgagg atctcccgcc gcaaggcgtc ccgttccgcg
     3301 tcccccagcg acagtccgga catctccgct tgcaggtact cctcggcgcg gtcccgccag
     3361 cggccgacat cctccgaaaa cgacaggccc gtcttccttg cgtcgaggcg cgacagtaca
     3421 gcctggtgca gtgtgcgtcg gatgtcaccg ttcatacaga tacctcctaa tgggtgtggt
     3481 gcttcggtgc tcacgggggg cttccactcc actatgttcg cgatcttcgc ccgctcactg
     3541 cgattccgct cgacgcaacc cttgcctcgc gccatacgct gcctcacctt gaaatccgtc
     3601 ccgggggggt aatcccttca gcgtgctgta cggggcgctg cgtggaaccc c
//
LOCUS       DASVJL010000251        10815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1727917, whole genome shotgun sequence.
ACCESSION   DASVJL010000251 DASVJL010000000
VERSION     DASVJL010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727917"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /gene="trpD"
                     /locus_tag="VF357_05130"
     CDS             <1..636
                     /gene="trpD"
                     /locus_tag="VF357_05130"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX7523561.1"
                     /translation="DLNADPVLVQRALDEVGITFMFAPRFHAAMKYAIGPRREIAIRT
                     IFNILGPISNPAGVRRQVVGVFSEELGDTYARVLAETGHLHAFVVHGTDGLDEVSLAA
                     PTIVWEVREGKVKRFLFDPRPFGFEYVPLAALRGGDAAANATILLGVLSGDPGPARQA
                     VLVNAAFATVAGGAAEDLREGVRAATRSIDSGAAMERLRGFLAILGKREAR"
     gene            654..1406
                     /locus_tag="VF357_05135"
     CDS             654..1406
                     /locus_tag="VF357_05135"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX7523562.1"
                     /translation="MSGVREELSARKALVSIADLLRTASMRGPAKDLLRTIPEAPGII
                     AEIKRASPSRGWIRRDLDAVATAAAYLAGGAWAVSVLTEGRSFGGSLTDLSDVRAAFP
                     EATLLRKDFVLDAYMLAESRAHGADLVLLMVSVLGEKTGEMAALAFSHRLEPLVEARD
                     AAEIAIAARSGARLIGINNRNLDTLTVDLSTGARLLPLLPAGVYPVVESGISTADQIR
                     DFHRDGARLFLIGESLAGSKDPADTIRGYVGK"
     gene            1450..2808
                     /locus_tag="VF357_05140"
     CDS             1450..2808
                     /locus_tag="VF357_05140"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX7523563.1"
                     /translation="MKVKFLLKDSEIPKTWYNVIADMPNPPAAVLHPGTGKPVSPDDL
                     APLFPMPLIEQEVSSQREIPIPEAVRDIYTLWRPTPMFRALRLEEAVGTKSKIYYKYE
                     GVSPAGSHKPNTSIPQAYYNKMAGRKRIATETGAGQWGSAMALAGSFFGLEVKVYMVK
                     VSYNQKPYRRMLMETWGAKVVPSPSRDTNSGRAILAADPDCPGSLGIAISEAVEDAAT
                     REDTSYALGSVLNHVCLHQTVIGLEAKEQMKLAGDYPDVVIGCCGGGSNLAGIGFPFL
                     RDKIAGKRNPRIIAVEPSSCPTLTKGIYAYDFGDTAKLTPMIKMYTLGHDFVPEGIHA
                     GGLRYHGDSALVSQLRHEGLLEAQAYGQIAVFESAMTFARTEGILPAPESSHAIHSAI
                     LEAKRADEEGKRKTILFNLSGHGFFDLTAYDDYLNGRLKDFDYPEEKIAEALHRLPKV
                     EG"
     gene            2819..3481
                     /locus_tag="VF357_05145"
     CDS             2819..3481
                     /locus_tag="VF357_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX7523564.1"
                     /translation="MFRIKICGVTRPEDATHAVACGAEAIGINFFQGSPRFVSAVLAR
                     EIVEAVAGRAEIVGVFVNEAPETIVALCGRLGIRRVQLHGDEPPGDASRIPLWRMRVV
                     HADRTPDLPALLAYPCEAFLLDAGGKGVYGGTGRNLAWGELADRFPGVVSTRSPGGPR
                     KPWFLAGGLTPSNIERAIIEARPCGVDVASGVESSPGRKDPGKVKTFIERAKAGFRIA
                     ET"
     gene            3468..4685
                     /gene="trpB"
                     /locus_tag="VF357_05150"
     CDS             3468..4685
                     /gene="trpB"
                     /locus_tag="VF357_05150"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX7523565.1"
                     /translation="MPKRRGNRTPDPAGHFGPFGGRYVAETLMSALIELEKAYREAWR
                     DRSFHRELDGLLKSYAGRPTPLYFAERLTEKAGGARIHIKREDLAHTGSHKINNTLGQ
                     GLLARRMGKRRIIAETGAGQHGVATATVCARMGIPCEIYMGEEDVRRQSHNVFRMRLL
                     GARVNPVTSGSRTLKDAMNEALRDWVTNVRTTYYLIGSTAGPHPYPVMVRDFQSVIGR
                     EALPQFRKAEGKLPTAVVACVGGGSNAMGIFTSFLRYPRVRLYGVEAGGLGLSSGKHG
                     ATLSRGKVGVLHGSKSFLLQDADGQVLEAHSISAGLDYPGVGPEHAWLKETGRAEYVS
                     VTDAQALSGFTLLSRYEGIQPALESSHAVAFAYRLAKAMRPSETVLVNLSGRGDKDMG
                     ILMELGESGRDGD"
     gene            4672..5466
                     /gene="trpA"
                     /locus_tag="VF357_05155"
     CDS             4672..5466
                     /gene="trpA"
                     /locus_tag="VF357_05155"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX7523566.1"
                     /translation="MTAIDAAFRRLRASGERGLVVYLTAGDPTPAASLRYLRAAADAG
                     ADIIEVGVPFSDPTADGPTIEAASRRALSSGMNVAGALELVRRFRRTHATPIVLFGYF
                     NPFLRYGWPSLCKEAREAGVDGFLVVDLPFEEREEALPSIRKAELDWIPLAAPTSGMA
                     RVRAVDRAGSGFLYLISVTGVTGVRKNLPSEMAAWTRKVAKATRLPVAVGFGISSPAM
                     AAAATRHADAAVVGSACVKIVERNGRGPRGPAELSRFVRSLKKALR"
     gene            5474..6322
                     /gene="accD"
                     /locus_tag="VF357_05160"
     CDS             5474..6322
                     /gene="accD"
                     /locus_tag="VF357_05160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX7523567.1"
                     /translation="MAIRLFRKKDESDRKIKIPEGMWIKCDACLEIIYKPEVERNLNV
                     CPKCVYHFRIPARERIRAVIDEGTFVEFGEEMESVDVLNFTDTKKYVDRLKEAKKKTG
                     RKEAVITGTGKIQGVEVALAVLDFEFQGGSMGSVVGEKIAITAEVALESRLPLIIFSA
                     SGGARMQEGTLSLMQMAKTSAALARLSDARLPFISVLTDPTTGGVAASFAMLGDVIIS
                     EPGALVGFAGPRVIEQTILQKLPEGFQRAEFLLEHGMVDMIVERSRLKATLSMILRYL
                     SGSGDR"
     gene            6319..7602
                     /locus_tag="VF357_05165"
     CDS             6319..7602
                     /locus_tag="VF357_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX7523568.1"
                     /translation="MIGGTGPSGPEIVRPGLSRILSAFSRSGHPERAFRILHVAGTNG
                     KGSTASFAYDVLRRLPLGPIGLYTSPHLIAPEERVRIDGKMISPRALRDGFRVAERLS
                     PAGNPLTWFEKMTWSATDWFRRKGVRIAVMETGLGGRWDATSACRPAVSVITTVGYDH
                     REWLGKTLGSIAAEKAGILREGIPLVTGRLRPAARAVVLQRARRLGCSAWELGRTFDW
                     REHRDGTIAVELPGLDLDRLRVRRIGGFQRDNAAVALAASWLLASGEGIGPAAFVAAA
                     TKALPASRWPGRWSPLPLRKNAGAWVDGGHNPEAASALAREISGVPPWVGGKRLVALW
                     SMLADKDARGYLRNLGRHLDGIVTYPLSHPRGAEKEALAEVCSKQGIACRLAGDFPEG
                     WRIARRWAGKGGVVLVCGSLAAAGDAYRYRVGFVP"
     gene            7623..9749
                     /gene="lptD"
                     /locus_tag="VF357_05170"
     CDS             7623..9749
                     /gene="lptD"
                     /locus_tag="VF357_05170"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX7523569.1"
                     /translation="MGLLLIAVGVSDVSAAARFPLPSTLKDFSKPGIRLDAPVHMTAD
                     TLSYDEETGVAVAEGNVEMVFGTRTMRADRVRYDSTTGEADLTGKVRYKDAGEEFSFD
                     RITINLDSETGVLYNGTIRISSSNYLISSREIKKTGKSTFLIEKGMITTCPCDPEPDW
                     KFEVRRARVALDQYAVGRDITFHIRGVPVLWLPWGAFPVKMSRQSGLLFPNFSSNRSR
                     GYALQVPYYQVINRWSDATVTLDVMSRRGYRPEAEYRFVLNPESEGAVRATMFRDRVE
                     DRSRGRYYGENVFRSGPFTANAILEVPTDPRYYLDLVDNNALVAARFARSEGFVSATG
                     AHSEQAVSILWNKNLQEIPGPDNTAQRVPEYTMTLLPEKIPVAGIDLSGDISATRFQR
                     RDGFDELRARGFGEVSRAIPLYRSFTLVPYLFADVLGTRFEQSPANGPSDHERVVPGG
                     GATLSADARKDIPERDLVHSAGASAGFRYVPKIHQDDLPITDRWSRLAPQSQFLFTLS
                     QRLLGVEDSAAPKEFLSFHIEWAYDLGGSEASGSPYVDPLAPYVRALRDQVDKGSGRP
                     PDKNNAPSDIYAKVGFNPIERWRFQGEALFDPVGSTFTIGAVSGEYKKDEDHRVLAEY
                     RLSRNLAEDVRGTFAWRLLRWLRLQAQADYSLRNGYLSEGGVGVSVFPRSDCWHVGFT
                     VDRKTQPDDTSIKLTFGLKGIGSVGN"
     gene            9790..10728
                     /locus_tag="VF357_05175"
     CDS             9790..10728
                     /locus_tag="VF357_05175"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7523570.1"
                     /translation="MRIMVTGGAGFIASHVAQAYVEAGHEVLVLDNLSSGKKENVPQG
                     ARFVFGDAGSETAVEAIRTFRPEVLCHHAAQINVRKSVADPVFDAKENIVSTLVLLEA
                     AREHGVRKVIFSSSGGAGYGEQEYFPADEKHPLKPVSPYGVAKVSVELYLQYYRVQFG
                     LEYTALRYSNVYGPRQDPHGEAGVVAIFCERLLKGQAALVNGDGLQTRDYVYVGDVVR
                     ANLAALARGDGLSVNIGTGIETDVNTLFRTLRDLSGSRQEEIHGPAMPGEQRRSVIEN
                     GMAFNELGWYPDISLNEGLALTLAYFRDKVKSSGTW"
BASE COUNT         1890 a         3356 c         3741 g         1828 t
ORIGIN      
        1 gatctgaacg cggaccccgt gttggtgcag cgcgccctcg acgaggtcgg gatcaccttc
       61 atgttcgctc ccaggttcca cgccgcgatg aaatacgcca tcggcccgcg acgggagatc
      121 gccatccgga cgatcttcaa catcctcggc ccgatcagca atcccgccgg cgtgcgacgg
      181 caggtcgtcg gcgtgttcag cgaggaactc ggggacacgt acgcacgggt tctcgcggaa
      241 accggccacc tgcacgcgtt cgtggttcac ggaaccgacg gactggacga ggtctccctc
      301 gccgcgccta cgatcgtctg ggaggtgcgg gaggggaagg tgaagcgttt cctcttcgac
      361 ccgcggccat tcgggttcga gtatgtcccc ctggccgccc ttcgcggagg ggatgccgcc
      421 gcgaacgcca cgatcctcct tggggttctc tcgggcgacc cgggtccggc gcggcaggcc
      481 gtgctcgtga acgccgcctt cgcaacggtc gccggcgggg ccgcggagga cctgcgggag
      541 ggggtgcggg cggcgacccg ctcgatcgac tcgggcgcgg cgatggaacg gcttcgggga
      601 ttcctcgcca tcctcgggaa gagggaagca cgctgaccca cctcgaacgc attctgtcgg
      661 gcgtccgcga ggaactttcc gcgcggaagg cgctcgtttc gatcgcggac cttcttcgga
      721 cggcttcgat gcgcggacct gcgaaggatc tcctccggac gatcccggaa gccccgggga
      781 tcatcgccga gatcaagcgc gcctctccat cgaggggatg gatccgtcgc gatctcgacg
      841 ccgtcgcgac cgctgccgcc tatctcgcgg gtggcgcttg ggcggtctcc gtactgacgg
      901 agggccgcag cttcggcggg tcgctcacgg acctttcgga cgtccgtgcc gcgttcccgg
      961 aggcgacgct tcttcggaag gatttcgtgc tggacgccta catgctggcg gagtcccggg
     1021 cgcacggcgc cgatctggtc ctgctcatgg tctccgtcct cggggagaag acgggggaga
     1081 tggcggcgct ggccttctcg caccgtctcg aaccgctggt ggaagcgcgc gacgcggcgg
     1141 agatcgcgat cgccgcccgg tccggggcgc ggctgatcgg tatcaacaac cggaacctgg
     1201 acactttaac cgtggacctg tccacggggg cgaggctgct cccccttctt ccggccgggg
     1261 tgtacccggt cgtggaaagc gggatctcga cggcggacca gattcgggac ttccacaggg
     1321 acggcgcccg cctcttcctg atcggggagt ccctggccgg gagcaaagac cccgctgaca
     1381 cgatccgcgg gtatgtggga aaatgaacgg agatccgatg atcgaccaag gcgcaaagga
     1441 gacgacacga tgaaagtgaa gtttctcctc aaggactcgg agataccgaa gacatggtac
     1501 aacgtcattg cggacatgcc gaaccctccg gcggcggtgc tgcacccagg taccggcaag
     1561 ccggtgagcc cggacgatct cgcccctcta ttccccatgc ccctgatcga gcaggaagtg
     1621 tcgtcgcaaa gggagatccc gatccctgag gcggtccggg acatctacac cctctggagg
     1681 ccgacaccga tgttccgcgc ccttcgcctc gaggaggcgg tggggacgaa gtcgaagatc
     1741 tactacaaat acgagggcgt cagtcccgcg gggagccaca agccgaacac ctccatcccc
     1801 caggcgtatt acaacaagat ggcggggcga aagaggatag cgacggagac gggggccggg
     1861 cagtggggtt ccgccatggc cctcgccggc tcgttcttcg gcctcgaggt gaaggtgtac
     1921 atggtgaagg tcagctacaa ccagaagccg taccgccgca tgctcatgga aacctggggg
     1981 gcgaaggtcg tgccgtcccc cagccgggac accaactcgg gacgagccat ccttgccgcg
     2041 gatcccgact gtcccggatc cctcggcatc gcgatcagcg aggcggtcga ggacgccgcg
     2101 acgcgtgaag acaccagcta cgccctcggg tccgtcctca accacgtctg tctccaccag
     2161 acggtgatcg gcctcgaggc gaaggaacag atgaagctgg ccggggatta ccccgacgtg
     2221 gtcatcgggt gctgcggcgg agggagcaac ctcgccggga tcggcttccc cttcctgcgg
     2281 gacaagatcg cggggaaaag gaatccacga atcatcgccg tcgagccgtc ctcctgcccg
     2341 acgctcacca aggggatcta cgcctacgat ttcggggaca ccgcgaaact cacgccgatg
     2401 atcaagatgt acaccctcgg acacgatttc gttcccgagg ggatccacgc gggggggctc
     2461 cggtaccacg gggactcggc gctggtcagc cagcttcgtc acgagggtct gctcgaggcc
     2521 caggcgtacg gtcagatcgc cgtcttcgag agcgcgatga ccttcgcccg gacggagggg
     2581 atcctgccgg cgcccgagtc gtcccacgcc atccactcgg cgatcctcga ggcgaagcgt
     2641 gcggacgagg aggggaagag gaagaccatc ctcttcaact tgagcggaca cgggttcttc
     2701 gacctgaccg cctacgacga ctacctcaac gggcgcttga aggatttcga ctatccggag
     2761 gagaagatcg cggaggcgct tcaccgcctc cccaaggtcg agggatagcg tctcccggat
     2821 gttccgcata aagatctgcg gggtgacccg tccggaggac gctacgcacg ccgtcgcgtg
     2881 cggggcggaa gcgatcggga tcaacttctt ccaggggtcg ccgcggttcg tttctgccgt
     2941 tctcgcccgg gagatcgtgg aggcggtggc gggtcgggcg gagatcgtcg gtgtgttcgt
     3001 gaacgaggcc ccggagacga tcgttgcgct gtgcgggcgg ctcgggatcc gccgggttca
     3061 gcttcacggg gacgagcctc ccggggacgc ctcccgcatc ccgctctgga ggatgagggt
     3121 ggtccacgca gatcgtaccc cggatcttcc ggcgctcctc gcgtatccgt gcgaggcgtt
     3181 cctgctcgac gccggtggaa agggcgtcta cggcgggacg ggtcggaacc tggcgtgggg
     3241 agagctggcg gaccggttcc cgggggtcgt gagtacgcgg agtccgggag gtccgcgaaa
     3301 accatggttt cttgcgggtg ggctcacacc ttcgaacatc gagcgtgcga tcatcgaggc
     3361 gcgcccgtgc ggagtagatg tcgcctccgg cgtggaatcg tcgccgggac ggaaagaccc
     3421 gggcaaggta aaaacgttca tcgaacgcgc gaaagcgggg tttcgcattg ccgaaacgta
     3481 gggggaacag gacgccggat ccggcgggcc acttcggccc cttcggggga aggtacgtcg
     3541 ccgagacgct gatgtccgcc ctgattgaac tggaaaaggc gtaccgggag gcgtggcgcg
     3601 accgctcctt ccatcgggag ctcgacggcc tcctgaagag ttacgccggc cggccgaccc
     3661 cgctgtattt cgcggagcgg ctgacggaaa aggcgggagg cgcccggatc catatcaagc
     3721 gggaggacct cgcccacacc gggtcgcaca agatcaacaa cacgctgggg caggggctgc
     3781 tggcgcgcag gatggggaag cggcggatca tcgccgagac gggcgcgggc cagcatggcg
     3841 tcgcaacggc caccgtctgt gccaggatgg ggatcccgtg cgagatctac atgggagaag
     3901 aggacgtccg gcgacagtcc cacaacgtct tccggatgcg cctgctcgga gcccgcgtca
     3961 acccggtgac ctcggggagc cggacgctga aggacgccat gaacgaggcg cttcgcgact
     4021 gggtgacgaa cgtccggacc acctactacc tgatcgggtc cacggcgggg ccccacccgt
     4081 acccggtgat ggtacgggat ttccagtcgg tgatcgggcg ggaagcgctg ccgcaattcc
     4141 ggaaagcgga agggaaattg ccgaccgccg tcgtggcgtg cgtggggggt gggagcaacg
     4201 cgatggggat cttcacctcc tttctgcggt atccccgggt gcggctctac ggcgtggagg
     4261 cgggaggtct cggcctctct tccgggaaac acggggccac actctcccgc gggaaggtcg
     4321 gcgtgctcca cgggagcaaa tcgtttcttc tacaggatgc ggacggacag gttctcgagg
     4381 cccattcgat ctccgcgggg ttggactacc ccggggtggg tccggaacac gcgtggttga
     4441 aggagaccgg cagggcggaa tatgtctccg tgacggacgc acaggcgctc tcgggcttca
     4501 ccctgctctc ccggtacgag gggatccagc cggcgctcga gtcgtcccac gccgtcgcgt
     4561 tcgcgtaccg cttggcgaaa gcgatgcggc cgtccgagac cgtcctggtg aatctctccg
     4621 ggaggggcga caaggacatg gggatcctga tggaattggg ggagagcggt cgtgacggcg
     4681 attgacgccg cgttccgacg cctccgcgcg tccggagaga ggggactcgt ggtctacctg
     4741 accgcgggag acccgacgcc ggcggcgtct ctccgatacc tccgggcggc agcggacgcc
     4801 ggggccgata tcatcgaggt aggcgttccc ttttcggatc ccacggcgga tggtcccacg
     4861 atcgaagccg cctcgcggcg ggcgctgtcg tcggggatga atgtcgcggg ggccctcgag
     4921 cttgtgcgga gattccgccg cacgcacgca acgccgatcg tgctcttcgg atatttcaac
     4981 ccttttctcc ggtacggctg gccctccctg tgcaaagaag ctcgggaggc gggtgtcgac
     5041 gggttcctgg tggtcgacct gcctttcgag gagagggaag aggcgctgcc gtcgatccgg
     5101 aaggcggagc tcgactggat ccccctggcc gcccccacga gcgggatggc gcgagtccgg
     5161 gcggtcgacc gcgccggctc cgggttcctg tacctgatct ccgtgaccgg tgtgaccggg
     5221 gtccggaaaa acctcccttc ggagatggcg gcgtggacca ggaaggtcgc gaaggcgacg
     5281 cggctgccgg tggccgtcgg attcggcatc tcctcgccgg cgatggcggc ggccgccacc
     5341 cggcacgccg acgcggcggt tgtcgggagc gcctgcgtca agatcgtcga aaggaacggc
     5401 cgcggtcccc gggggcccgc ggagctttcc cggttcgtcc gatccctcaa aaaggcactg
     5461 aggtgacatg gccatggcga ttcgactctt ccggaaaaag gacgagagcg acaggaagat
     5521 caagatcccc gaggggatgt ggatcaagtg cgacgcctgc ctcgagatca tctacaagcc
     5581 cgaggtggaa cggaacctga acgtctgccc caaatgcgtc taccacttcc ggatccccgc
     5641 ccgggagcgg atccgcgccg tgatcgacga ggggaccttc gtcgagttcg gcgaggagat
     5701 ggagtccgtg gacgtcctga acttcacgga cacgaagaag tatgtcgatc gcctgaagga
     5761 ggcgaagaag aagacggggc gcaaggaagc ggtcatcacg gggaccggga agatccaggg
     5821 ggtcgaggtg gccctggcgg tcctcgattt cgagttccag ggcggttcga tgggaagcgt
     5881 cgtcggggag aagatcgcca tcacggcgga agtcgccctc gaatcccgcc ttccactgat
     5941 catcttcagc gcatccgggg gggcgcgcat gcaggagggg accctctcgc tgatgcagat
     6001 ggccaaaacg agcgcggccc tcgcacgtct ctccgacgca cgacttcctt tcatcagcgt
     6061 gctgaccgat ccaaccaccg gcggggtggc ggcgagcttc gcgatgctgg gggacgtcat
     6121 catctccgag ccgggggcgt tggtcgggtt cgcggggccg cgggtgatcg agcagacgat
     6181 cctgcagaaa ctgccggagg ggttccagcg cgccgagttt ctcctcgaac acgggatggt
     6241 ggatatgatc gtggaacgta gccggctcaa ggcgacgctt tcgatgatcc tgcgctactt
     6301 gtcgggttcc ggggatcgat gatcggggga accggacctt ccggcccgga aatcgtccgc
     6361 cccgggctgt cccggatcct gtcggcgttt tcccggtccg ggcatccgga aagggcattc
     6421 cgcatcctcc acgttgcggg gaccaacggc aaggggtcca ccgcctcttt cgcgtacgac
     6481 gttcttcgcc gtttgccgct gggccccatc ggcctgtaca cctccccaca ccttatcgcg
     6541 ccggaagagc gggtccgcat cgacgggaaa atgatctctc cacgcgctct tcgtgacggt
     6601 ttccgcgttg cggagcgtct ctctcctgcc gggaatccac tgacctggtt cgagaagatg
     6661 acctggtccg ccaccgactg gttccggcgc aagggggtcc ggatcgcagt catggagacg
     6721 gggctcggcg gtcgatggga cgcgacgagc gcgtgccgcc cggcggtctc ggtcatcacg
     6781 accgtcggat acgaccaccg ggagtggctc gggaaaacgc tcggatcgat cgccgcggaa
     6841 aaggcgggga tccttcgaga gggcatcccg ctcgttaccg gacggttgcg gcctgccgcc
     6901 cgcgccgtcg tcctgcagcg cgcacggcgg ctagggtgca gcgcctggga actcggccgc
     6961 actttcgact ggagggagca tcgggacggg acgatcgcgg tggagcttcc cggcctcgat
     7021 ctcgatcggt tgcgggtaag gagaatcggc gggttccagc gcgataacgc cgcggtcgcg
     7081 ctggccgcct cgtggctcct cgcctccggc gaggggatcg ggcccgccgc cttcgtcgcg
     7141 gcggcaacaa aggcgctccc tgcctcgcga tggcccggtc gatggtctcc gctcccgcta
     7201 cgaaagaacg ccggggcgtg ggtggacggc gggcacaacc cggaggcggc gtccgcgctg
     7261 gcgcgggaga tctcgggggt tcccccctgg gtcgggggga aacggctggt tgccctatgg
     7321 agcatgcttg ccgacaagga cgccaggggg tacctgcgga atctcggacg acacctcgac
     7381 ggaatcgtga cgtatccgct atcgcatccg cgcggggcgg aaaaggaggc actcgcggaa
     7441 gtgtgctcga aacaggggat tgcctgccgc ctcgcggggg acttcccgga aggatggcgg
     7501 atcgcgcgtc ggtgggccgg gaagggaggc gtcgtgctcg tgtgcggttc gctcgccgcc
     7561 gcaggggacg cttaccggta ccgcgtcggt ttcgtacctt gaatcctctc caacgcctcg
     7621 tcatgggtct tctgctgatc gccgtgggcg tctcggacgt ttccgcggcg gcgcgttttc
     7681 cccttccctc caccttgaag gacttctcga aaccggggat tcgtctcgac gccccggttc
     7741 acatgaccgc cgacacgctt tcctacgacg aggagaccgg ggtcgccgtg gcggagggga
     7801 acgtcgaaat ggtgttcgga acgcggacga tgcgtgccga ccgggtccga tacgattcga
     7861 cgaccgggga ggcggacttg accgggaagg tgcggtacaa agacgcggga gaagagttct
     7921 cgttcgaccg gatcacgatc aatctcgact cggagacggg tgtcctctac aacggcacca
     7981 tccggatcag cagcagcaat tatctcatct cgagccggga gatcaagaag accggaaaaa
     8041 gtacgttcct gatcgaaaaa gggatgatca ccacttgccc gtgcgaccca gagccggact
     8101 ggaagttcga ggtgcgccgg gcgcgggttg ctctcgacca gtacgcagtc gggagagata
     8161 tcacgttcca catccgggga gttcccgtcc tgtggctgcc gtggggggcg tttccggtca
     8221 agatgtcgcg tcagagcggg ctcctgttcc cgaacttctc ctccaaccgg tccagaggat
     8281 atgcgctgca ggtgccgtac taccaggtga tcaaccgctg gagcgacgcc acagtgacgc
     8341 tcgatgtgat gagccgccgc ggataccgtc cggaagccga gtaccggttc gtattgaatc
     8401 cggagtcgga gggggcggtt cgcgcgacga tgttccggga ccgggtcgaa gatcggagcc
     8461 gggggcgcta ttacggggaa aacgttttcc gttccggccc cttcaccgcg aacgcgatcc
     8521 tggaagtgcc taccgatccc cggtactacc tcgacctggt cgacaataac gctctcgtgg
     8581 cggcgcgctt cgcacgatcg gaagggttcg tgtccgccac gggagcgcat tcggaacaag
     8641 cggtatcgat cctgtggaac aagaaccttc aggagattcc cgggccggac aacaccgcgc
     8701 agcgggtgcc ggagtacacg atgacgctcc tgcccgaaaa gatccctgtc gcggggatcg
     8761 atctttccgg agatatctcc gcaacccggt ttcagcgcag ggatggtttc gacgaacttc
     8821 gcgcaagggg gttcggggag gtttcacggg cgattccgct gtaccgttcc ttcaccctgg
     8881 tgccgtacct ctttgcggac gttctcggca cccggttcga acagtctccg gcgaatggcc
     8941 catccgatca cgagcgcgtc gtgcccggag gcggggcgac gctttcggcg gatgcccgca
     9001 aggatatccc ggagagggat ctcgtacatt cggccggggc ctcggcgggg ttccgttacg
     9061 ttccgaagat ccaccaggac gatcttccca tcaccgaccg atggtcccgt ctggcgcccc
     9121 agagccagtt tctcttcacg ctttcacagc gccttctcgg ggtggaggac agcgcggcgc
     9181 cgaaggaatt cctgtcgttc cacatcgagt gggcctatga tctcggaggg agcgaggcgt
     9241 cggggagccc gtacgtcgat ccgctcgccc cttatgtacg ggcgctgcgg gaccaggtcg
     9301 acaagggatc gggtcgtcct ccggataaga acaatgcccc ctcggacatc tacgcaaagg
     9361 tcgggttcaa ccccatcgag cggtggcgat tccagggcga ggcgctcttc gatccggtgg
     9421 ggagcacctt cacgatcggt gcggtcagcg gggaatataa aaaagacgag gatcaccggg
     9481 tcctcgccga gtaccggctt tcccgcaacc tggcggaaga cgtacgggga acattcgcgt
     9541 ggcgactgct ccgctggctc cgactgcagg cgcaggcgga ctactccttg aggaacggct
     9601 acctttccga ggggggcgtg ggggtttcgg tgtttccgcg aagcgactgc tggcacgtcg
     9661 gtttcaccgt cgatcggaag acgcagccgg acgacacgag cataaaattg acgttcgggc
     9721 tcaaggggat cggttccgtg ggaaactgaa cgtcggcaac ggacaatatc gaggtcgaag
     9781 ggagaatcga tgcggatcat ggtcaccggg ggggcgggtt tcatcgcatc ccatgtcgcg
     9841 caggcgtacg tcgaagcggg tcatgaggtt ctggtgctgg acaacctttc ctccgggaaa
     9901 aaggagaacg ttccccaagg cgctcggttc gtcttcggag acgcgggatc ggaaaccgcc
     9961 gtcgaggcga tccggacctt ccggccggag gtcctctgcc accacgcggc ccagatcaac
    10021 gtgcgcaagt ccgtggcgga tcccgtcttc gacgcgaagg agaacatcgt gagcacgctc
    10081 gttctccttg aggcggcgcg ggagcatggg gtccggaagg tgatcttctc ctcctccgga
    10141 ggcgccgggt acggcgagca ggagtacttc cccgcggacg aaaagcaccc gctgaagccc
    10201 gtttccccat acggggtagc gaaggtgtcc gtggaactct acctccagta ctaccgtgtc
    10261 cagttcgggc tggaatacac ggcgttgcgg tactcgaacg tgtacgggcc gcgacaggat
    10321 ccccacggag aggccggcgt ggtcgccatc ttctgcgagc ggctgctgaa ggggcaggcc
    10381 gccctcgtca acggcgacgg attgcagacc cgcgattacg tatacgtagg ggatgtcgtg
    10441 cgcgccaatc tcgcggcgct cgctcgcggg gacgggctct ccgtaaacat cggcaccggg
    10501 atcgagaccg acgtgaacac cctcttccgg acgctgcggg acctctccgg gagccgccag
    10561 gaggagatcc acgggccggc gatgccgggg gagcagcgcc ggtcggtcat cgaaaacggg
    10621 atggccttca acgaactggg gtggtatcct gatatttcgt tgaacgaagg gctcgccctc
    10681 acgctggcgt acttccgaga caaggtgaaa tcctccggaa cctggtagac atgcgaatag
    10741 agaacatccg caatttttcc atcatcgccc acatcgacca cgggaagtcc accctggccg
    10801 accggctcct cgaga
//
LOCUS       DASVJL010000252         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_226837, whole genome shotgun sequence.
ACCESSION   DASVJL010000252 DASVJL010000000
VERSION     DASVJL010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226837"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VF357_05180"
     CDS             complement(<1..672)
                     /locus_tag="VF357_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7523571.1"
                     /translation="MDPKKDYYRILGVPEGASAEEIRKAFRRLAKKHHPDVNPGNKAA
                     ESRFKEANEAHEVLSDRKKREEYDAIRQGGFAGGFQGAGPFGGGGHAPGGFRAESFDF
                     GDLFGDALRGGGARFSHPGRGSDIRVELSVDFLDMVRGAVREIRYPRPRVCGQCGGTG
                     RAGRRGCPACYGRGVTDSEERVKIRIPAGARDGATIRVPSKGEERAAPGESGDLQVEL
                     RMLAHP"
     gene            complement(740..1192)
                     /locus_tag="VF357_05185"
     CDS             complement(740..1192)
                     /locus_tag="VF357_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7523572.1"
                     /translation="MAIVRFWDPMKELSSRQNRMNRVFEETFGSPLYRGEQPGVGTWS
                     PAVDIFETGDEIIVKAEVPGLAKDQVRVEVENGVLTLHGERKFEREVKEENYHRVERT
                     YGAFHRSFSLPGSVDAERVRAEMKDGILEVHLGKREQAKPKQINVAVK"
     gene            complement(1259..>2854)
                     /gene="dnaK"
                     /locus_tag="VF357_05190"
     CDS             complement(1259..>2854)
                     /gene="dnaK"
                     /locus_tag="VF357_05190"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX7523573.1"
                     /translation="GKEYTPQQVSAMILGKLKKAAETYLGEEVKQAVITVPAYFNDSQ
                     RQATKDAGTIAGLEVLRIINEPTAAALAYGLDRKKNELIAVFDFGGGTFDISILEVGD
                     NVVEVKSTNGDTHLGGDNVDQRLIEWIIAEFLKDQGIDLSKDRTVLQRLKEGAEKAKI
                     ELSSVMETEISLPFITADASGPKHLQLKLSRAKFEQMVQDILDRTMKPCEMAVRDAGV
                     PVGKIDEVVLVGGSTRIPKVVEMVKTFFGKDPHQGVNPDEVVAAGAAVQAGVLGGTVK
                     DLLLLDVTPLSLGIETLGGVMTKLIERNTTIPVRKSEVFTTASDSQPSVEIHVLQGER
                     EMAGANRTLGRFHLDGIPAAPRGVPQVEVTFDIDANGILHVNAKDKGTQKEQKITITA
                     STGLDKNDIDKMVKEAELHAEEDRKRKAAIEARNHLDSLVYNTEKTLKEHRDKVPAET
                     AAKVEAALAEAKEALKSEDEAVLKAATEKLIHESHALAEHMYKQASSSQGDGAAGGGS
                     APGEGKAPEGDVVDAEYEDPAKK"
BASE COUNT          375 a         1080 c          839 g          560 t
ORIGIN      
        1 cggatgcgcc agcatgcgca actcgacctg caggtcgccg ctttcccccg gagccgcccg
       61 ctcctctccc ttcgaaggga cgcggatcgt ggcgccgtcc ctcgctcccg cggggatccg
      121 gatcttgacg cgctcctcgg aatcggtcac cccgcgtccg tagcatgcag ggcacccccg
      181 ccgcccggcg cggccggttc ctccgcactg cccgcaaacg cggggccggg gataccggat
      241 ctcgcggacc gccccccgta ccatgtcgag gaagtctacg gacagctcga cccggatgtc
      301 gctcccccgg ccggggtggg agaacctcgc tcccccgccg cgaagcgcat cgccgaaaag
      361 atccccgaag tcgaacgact ccgcccggaa ccccccgggg gcgtgtcccc cgccgccgaa
      421 cggtccggcg ccttgaaatc ctcccgcgaa ccctccctgg cggatcgcgt cgtactcttc
      481 ccgcttcttc cggtcgctca ggacttcgtg ggcctcgttc gcctccttga agcgcgactc
      541 ggccgccttg ttgccggggt tgacgtccgg atggtgcttc ttcgccagcc gccggaacgc
      601 cttccggatc tcctccgccg aggcgccttc cgggaccccc aggatccggt aatagtcctt
      661 cttcgggtcc atcagcccct cccgacggcg ccgggttccg cggggcgaac cccctgcgga
      721 acccgaccga tgcgcggcgt cactttacgg caacgttgat ctgcttcggc ttcgcctgct
      781 cccgcttccc gaggtgcacc tcgaggatgc cgtccttcat ctcggcgcgg accctttcgg
      841 cgtccacgga gcctggaagg gagaaggacc ggtgaaaggc gccgtaggtg cgctccaccc
      901 ggtggtagtt ctcctccttg acctcccgct cgaacttccg ctccccgtgc agcgtcagca
      961 cgccgttctc tacctcgacg cgaacctggt ccttcgcaag gcccgggacc tcggccttga
     1021 cgatgatctc gtcgccggtt tcgaagatgt ccacggccgg agaccacgtc ccgacccccg
     1081 gctgctcgcc gcgatacagc ggcgacccga aggtctcctc gaacacccgg ttcatccggt
     1141 tctgccgcga cgacaactcc ttcatcgggt cccaaaaccg cacgatcgcc atggtcctcc
     1201 tcctctcccc cgtcgccggc tggccgccgc tacgggtcgc gttgtttata taacccgcct
     1261 acttcttcgc gggatcctcg tattcggcat cgacgacgtc cccctcggga gccttcccct
     1321 caccgggagc gcttcctccc ccggccgccc cgtcgccctg cgaggaagac gcctgcttgt
     1381 acatgtgctc cgcgagggca tgagactcgt ggatcagctt ttccgtagcg gccttgagca
     1441 cggcctcgtc ctccgacttc aaggcctcct tcgcctccgc gagcgcggcc tccaccttgg
     1501 ccgccgtctc cgccgggacc ttgtccctgt gctccttcag cgtcttctcc gtgttgtaca
     1561 ccagcgaatc gaggtggttg cgcgcctcga tcgccgcctt ccgtttccgg tcttcctccg
     1621 cgtgcagctc ggcctccttc accatcttgt cgatatcgtt cttgtcgagc ccggtcgaag
     1681 cggtgatcgt gatcttctgc tccttctgcg ttcccttgtc cttcgcgttc acgtgcagga
     1741 tgccgttcgc gtcgatgtcg aaggtcacct cgacctgcgg gacgccgcgc ggcgccgccg
     1801 ggatcccgtc gaggtggaac cgtccgagcg tccggttggc accggccatc tcgcgctccc
     1861 cctgaaggac gtggatctcg acgctcggct ggctgtccga ggcggtcgtg aagacctcgc
     1921 tcttgcgcac ggggatcgtc gtgttccgct cgatcagctt cgtcatcacg ccccccagcg
     1981 tctcgatccc cagcgacagc ggggtcacgt cgaggaggag caggtccttc accgtcccgc
     2041 cgagcacgcc cgcctgcacc gccgcgcccg ccgcgacgac ctcgtcgggg ttgacgccct
     2101 ggtggggatc cttgccgaag aacgtcttca ccatctccac gaccttcggg atgcgggtcg
     2161 agccgcccac gaggacgacc tcgtcgatct tgccgacggg gacgcccgcg tcgcgcaccg
     2221 ccatctcgca cggcttcatc gtccggtcga ggatgtcctg gaccatctgc tcgaacttcg
     2281 cccgggaaag cttcagctgc aggtgcttcg gccccgacgc gtccgcggtg atgaacggca
     2341 ggctgatctc ggtctccatc acggaggaga gctcgatctt cgccttctcc gccccttcct
     2401 tcagccgctg aagaacggtg cggtccttcg acaggtcgat tccctggtcc ttcaggaact
     2461 cggcgatgat ccattcgatc agccgctggt cgacgttgtc tccgcccagg tgggtgtccc
     2521 cgttcgtcga cttcacctcg acgacgttgt cccccacctc gaggatggag atgtcgaagg
     2581 tgccgccgcc gaagtcgaag acggcgatca gctcgttctt cttccggtcg aggccgtacg
     2641 caagcgccgc cgccgtgggc tcgttgatga tccggagcac ctcgaggccg gcgatcgtcc
     2701 cggcgtcctt ggtcgcctgc cgctgggagt cgttgaagta ggccggaacg gtgatgaccg
     2761 cctgcttcac ctcctcgccc aggtacgtct ccgccgcctt cttgagcttc ccgaggatca
     2821 tcgcggagac ctgctgcggg gtgtactcct tccc
//
LOCUS       DASVJL010000253         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1446954, whole genome shotgun sequence.
ACCESSION   DASVJL010000253 DASVJL010000000
VERSION     DASVJL010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446954"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VF357_05195"
     CDS             complement(<1..1104)
                     /locus_tag="VF357_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7523574.1"
                     /translation="MIPARLLPVALLLAVMLPGAAAAQEGEDLPRIFLEKKVYSDTAG
                     GKQSFFERHTVEKGDTLWKILERKEVLTPDRYAERIKGFRRANPKVADPSRLTPGQEI
                     LVPSGGRPEEKDDGRTVGYTVKRGDSLSKILSSRDVPLRQRKKYLDAIREINPSVTDV
                     DRIVAGKTLHLPTEGYFTEAKPPAEPEKTMVAMEPVKAGIAPEPAAPGQSAVPAKGPK
                     PEKIASPEVGLSAVTPLAKATPAPEVPPAPVLTRDIAAGPGQDALAAGKPEAELLVPK
                     PVSPTASVAETGKAKPAEEAVIPPARSPYRGLLSDLFLAIGEKWIDRGTMYLPLPSGG
                     EAVLQLSDFPVVRFSGGTEALIDFRGGIPPRVAD"
     gene            complement(1101..1886)
                     /locus_tag="VF357_05200"
     CDS             complement(1101..1886)
                     /locus_tag="VF357_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523575.1"
                     /translation="MPTTIYDLNRMSEVERSALLSLLVPARLFSMFSIDPRTFRNPSG
                     IECVKFTCPDEMPFFQIDVRRAPADKDAAYFLDVSTSAFGQMEISFIIVNDPDGERFG
                     IDRDENGHETYFGTARRNVPEELRAMEAGLAPGQVRRGLRMMSEMVACWDAFFGRLGY
                     KFYFLEPLGYNSAILYERAGFQYLKGKEKMVWTDREFLPGGLLRERLDGSTPFRRPSL
                     ARTVRGRAWAIHDGILDEPWESPKMYKTVGVHAGIDTFTGEKY"
     gene            complement(1896..>2520)
                     /locus_tag="VF357_05205"
     CDS             complement(1896..>2520)
                     /locus_tag="VF357_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7523576.1"
                     /translation="FGTVGKALPGTEIRIAADGEILVRGSHIFREYFNDPAATREAIE
                     PDGWFHTGDIGALDEEGFLRITDRKKDIIVTSGGKNIAPQNLENLLKNDRYISQAFVF
                     GDRKKYLTALLTLAPEEIVVWAARQGIPDRSVEALAKHPEVLQLMRERVNEVNRGLAS
                     FEQVKKFALLETDFSQETGELTPTLKVRRKVVVEKYGRILDDLYGKD"
BASE COUNT          404 a          880 c          785 g          451 t
ORIGIN      
        1 atccgcaacg cggggcggga tgccgccgcg gaaatcgatg agcgcctccg ttcccccgga
       61 gaatcgcacg acggggaaat cggacagctg aagcaccgcc tcgcccccgg aagggagggg
      121 aaggtacatc gttccccgat cgatccactt ttcgccgatc gccaggaaga ggtccgacag
      181 gagcccccgg tacggagacc gcgccggcgg gatgacggcc tcctccgcgg gcttcgcctt
      241 ccccgtctcc gcgaccgacg cggtcgggga gacgggcttg ggaaccagca gctccgcctc
      301 cggcttcccg gcggccaagg cgtcctggcc cggaccggcg gcgatatcgc gcgtcaacac
      361 cggcgcggga ggaacctccg gtgcgggcgt agccttcgcc aggggcgtaa cggccgagag
      421 cccgacctcc ggcgacgcga ttttctccgg cttcgggcct ttcgccggca cggcagattg
      481 ccccggcgcg gcaggttccg gggcgattcc cgccttcacc ggttccatgg cgaccatcgt
      541 cttttccggc tccgccggcg gcttcgcctc cgtgaagtac ccttctgtcg gaagatggag
      601 cgtcttcccg gcgacgatcc gatccacgtc cgtgaccgac ggattgatct cccggatcgc
      661 gtcgaggtac ttcttcctct gtcggagcgg aacgtcgcgg gaggagagga tcttcgaaag
      721 agagtctccc cgctttaccg tgtagccgac cgtccttccg tcgtctttct cctccggtct
      781 tccgccggac ggaacgagga tctcctggcc cggtgtaagg cgcgacgggt cggcgacctt
      841 cggattggcc cgccggaatc ccttgatgcg ttccgcgtac cggtccgggg tgaggacctc
      901 cttccgctcg aggatcttcc agagcgtgtc ccccttctcc accgtgtgcc tttcgaagaa
      961 gctctgcttc ccgcccgccg tgtcggaata gaccttcttc tcgaggaaga tccggggaag
     1021 atcctccccc tcctgggccg ccgccgcgcc cggcagcatc acggcaagaa ggagggcgac
     1081 aggaagaagg cgcgccggaa tcaatatttc tccccggtaa aggtgtcgat gcccgcgtgc
     1141 acgccgaccg tcttatacat cttggggctt tcccacggct cgtcgaggat cccgtcgtgg
     1201 atcgcccacg cccgcccgcg gacggtccgc gcgagcgaag gccgccggaa cggcgtgctc
     1261 ccgtcgagcc gctcgcgcag cagtccgccg ggaaggaact cccggtcggt ccataccatt
     1321 ttttccttcc ccttcaggta ctggaacccc gcgcgctcgt acaaaatcgc gctgttgtac
     1381 cccagcggct cgagaaagta gaacttgtac cccagccggc cgaagaaggc gtcccagcac
     1441 gccaccatct ccgacatcat ccgcaagcca cggcgcacct gccccggcgc cagtcccgcc
     1501 tccatcgccc ggagctcctc cggcacgttc cgccgcgcgg tcccgaagta cgtctcgtgg
     1561 ccgttttcgt cccggtcgat cccgaaccgc tccccgtcgg gatcgttgac gatgatgaag
     1621 gagatctcca tctgcccgaa cgccgaggtc gacacgtcga gaaagtatgc cgcgtccttg
     1681 tccgcgggag ccctccggac gtcgatctgg aagaacggca tctcgtccgg gcaggtgaac
     1741 ttcacgcact cgatccccga tggattccgg aacgtccgcg ggtcgatcga gaacatcgaa
     1801 aagaggcgcg ccggtacgag gagcgacaag agcgcggacc gctccacctc cgacatacgg
     1861 ttgagatcat agatcgtggt gggcaacggt cccggtcaat ccttcccgta gagatcgtcg
     1921 agaatccgtc cgtacttctc gaccaccacc ttgcggcgga ccttgagcgt gggcgtcagt
     1981 tcgccggttt cctgggagaa gtccgtttcc agcagcgcga acttcttcac ctgctcgaag
     2041 gaggccagcc cccggttgac ctcgttgacc cgctcgcgca tcaactgcag cacctcggga
     2101 tgcttcgcca gcgcctcgac gctgcgatcc gggatcccct gccgggccgc ccagacgacg
     2161 atctcctcgg gggcgagcgt gaggagcgcg gtcagatact tcttccggtc gccgaatacg
     2221 aacgcctggc tgatgtaccg gtcgttcttg aggagattct ccaggttctg gggggcgatg
     2281 ttctttcccc ccgaagtgac gatgatgtcc ttcttccggt cggtgatgcg gaggaacccc
     2341 tcctcgtcga gggccccgat gtcccccgtg tggaaccagc cgtccggttc gatcgcctcc
     2401 cgggtggcgg cggggtcgtt gaaatactcg cggaagatgt gcgacccccg cacgaggatc
     2461 tcgccgtccg cggcgatgcg gatctccgtg ccggggagcg ccttccccac ggtgccgaac
//
LOCUS       DASVJL010000254         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1541038, whole genome shotgun sequence.
ACCESSION   DASVJL010000254 DASVJL010000000
VERSION     DASVJL010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541038"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VF357_05210"
     CDS             <1..754
                     /locus_tag="VF357_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7523577.1"
                     /translation="MLIDINPKDYDYSSLRFCTGGGEALTAKTYLDWKEKFGLEIYEG
                     LGTTEMMFVFISAAVTKKVKPGPIGTACPGYDVRVVNESFEQVKPGEVGKMIVRGPTG
                     TIYWKDNEKQKSAVRDGWCLAGDVVTMDEDGYVQFLSREDDLIKSSGYRIGPEEIEEA
                     LVMHPSVADAGVVGVPDPVIGQKTKAFVALKPGVTPSEALKKELIEHCKGKIAVYKLP
                     REIEFIDAMPRTAVGKLLRRILRQQEIEKQKK"
     gene            complement(841..>1827)
                     /locus_tag="VF357_05215"
     CDS             complement(841..>1827)
                     /locus_tag="VF357_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7523578.1"
                     /translation="TLFSAMLLAFALEPLVHLLCARTLLRRHHASGIVVFLFVAMLYG
                     LFYATYLRAESFFAEIPAIAEKIRSAPMVEAIVNRAEEVNRVFAEAGRRIVPPAAVSE
                     RPKPVPPFVVRDPESFTGTLLQGVGSLGGVFFALGFIPFLVYFILADREPLTRRTREL
                     FPEEHRETVGEILLDIEWMMQKFLVGNALIALFLSVATVLVYLLVGLPYPVFLGILSG
                     ILSTVPYLGLPLALLPGVVVGLVSFESGGPFLALIALVTVLHIVAANYLTPKLVGRGV
                     RLNATASTVALLFFGWLWGAMGMVLGIPILAVLKCIFDNVPSTRRIGLFLGE"
BASE COUNT          374 a          572 c          637 g          244 t
ORIGIN      
        1 gatgctgatc gacatcaacc cgaaagacta cgactactcc tccctccggt tctgcaccgg
       61 cggcggcgag gcgctcaccg ccaagacgta cctcgactgg aaggagaagt tcgggctgga
      121 gatctacgag gggctcggca cgaccgagat gatgttcgtg ttcatctccg cggcggtcac
      181 gaagaaggtc aagcccggcc cgatcgggac ggcgtgcccc ggctacgatg tgcgcgtggt
      241 gaacgagagc ttcgagcagg tcaagcccgg cgaggtcggc aagatgatcg ttcgcgggcc
      301 cacggggacg atctactgga aggacaacga gaagcagaag tcggcggtcc gcgacggctg
      361 gtgcctggcg ggcgacgtgg tcacgatgga cgaggacggg tacgtccagt tcctctcccg
      421 cgaggacgac ctgatcaagt cgtccggcta ccggatcggt cccgaggaga tcgaggaggc
      481 gttggtgatg cacccgtccg tcgccgacgc gggcgtggtc ggcgttcccg acccggtcat
      541 cgggcagaag accaaggcgt tcgtcgcgct gaagcccggc gtcacgccgt ccgaggcgct
      601 gaagaaggag ctcatcgagc attgcaaggg gaagatcgcc gtctacaagc tgccgcggga
      661 aatcgagttc atcgacgcga tgccgcggac cgcggtcggc aagctcctgc ggcgcatcct
      721 gcgccagcag gaaatcgaga agcagaagaa gtaaggcagg ggactgccgc gcccgtcggc
      781 gcggcggcgg caggagggcc cgggggcgtc acgccccggg cttttttttg acgtgccccg
      841 ttattccccg aggaacagcc cgatccgccg cgtggacggg acgttgtcga agatgcattt
      901 caggacggcc aggatcggga tccccagcac catccccatc gccccccaca gccagccgaa
      961 gaagagcagg gccaccgtcg aggcggtcgc gttcagccgc acccctctgc ccaccagctt
     1021 cggcgtcagg tagttcgccg ccacgatgtg aagcacggtc accaaggcga tcagggccag
     1081 gaagggcccc cccgactcga aggagacgag gccgacgacg acgcccggca gcagcgcgag
     1141 cggcaggccg aggtacggga cggtgctgag aatcccgctc aggatcccca aaaacaccgg
     1201 gtacggaagc cccacgagca ggtacaccag gacggtcgcc acggagagaa agagggcgat
     1261 caatgcgttg ccgacgagga atttctgcat catccactcg atgtcgagga ggatctcccc
     1321 caccgtctcg cggtgctcct ccgggaagag ttcccgcgtc cgccgggtga gcggttcacg
     1381 gtccgccagg atgaagtaga cgaggaaggg gatgaagccg agggcgaaga aaacgccgcc
     1441 cagggaaccg accccctgga gcagcgtccc cgtgaacgat tccggatccc gaacgacgaa
     1501 cggcgggacg ggtttcgggc gctcggaaac cgccgccggg ggaacgatgc gccggcccgc
     1561 ctcggcgaag acgcggttca cctcctcggc cctgttcaca atggcctcga ccatcggcgc
     1621 ggagcggatc ttttcggcga tcgcggggat ctccgcgaaa aagctctccg cgcgcaggta
     1681 cgtggcatag aaaagcccgt agagcatcgc gacgaacagg aagaccacga tgccgctggc
     1741 atgatggcgc cgcaagaggg tccgcgcgca gagcaggtgg accagcggtt cgagggcgaa
     1801 ggccagcagc atggcggaga acagggt
//
LOCUS       DASVJL010000255        12862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1541241, whole genome shotgun sequence.
ACCESSION   DASVJL010000255 DASVJL010000000
VERSION     DASVJL010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 12862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12862
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541241"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..942)
                     /gene="panB"
                     /locus_tag="VF357_05220"
     CDS             complement(148..942)
                     /gene="panB"
                     /locus_tag="VF357_05220"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX7523579.1"
                     /translation="MQKTNILDLARMKAEGAKIVMITAYDALFARIFDDAGVDVILVG
                     DSLGMVVLGHPDTLNVTMEDMVRHTEAAARGRKRAFLAADMPFLSYQACVADAIRKAG
                     RLIQAGAEAVKLEGGRIAAETIRAIAAADIPVMGHIGLTPQSVHRMGGYRVQGKTDPQ
                     RERLLDDAAAVQEAGAFSVVLEGIPAALAAEITRTLSIPTIGIGAGVGCDGQVLVMHD
                     LLGLYDEFRPKFVKRFGELRKPVEDAVGAYAAAVRDGSFPGKEHSF"
     gene            complement(1091..1741)
                     /locus_tag="VF357_05225"
     CDS             complement(1091..1741)
                     /locus_tag="VF357_05225"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX7523580.1"
                     /translation="MTEPKTPRYIAIEGPIGVGKSSLAKILAQKYASRLVKEEIAGNP
                     FLERFYENPRKFAFQTQLFFLLSRYRQQRELAQGDLFEAGLVCDYILAKDKIFALINL
                     EDDEVSLYESIYKLLVSTLPKPDLVIYLQARPEVLLSRVRKRGIAYERNISLDYLRTL
                     SDAYNEYFFHYNETPLLVVNTSEIDFVESPRDLEHLVREVKSVKRGTQHYIPLGSR"
     gene            complement(1991..3541)
                     /locus_tag="VF357_05230"
     CDS             complement(1991..3541)
                     /locus_tag="VF357_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7523581.1"
                     /translation="MKTIASKLSVPLVAILIVGIAVMMFIINYSLKKSYEEDAVTSLE
                     KTQYLMFTAINAVMKNSEGDSIKKGIKDITDKNDGNIKIIPSKEVFDGNNNEDENQKT
                     VRERKVKKIINSSESIKGIFPYIAKEECLDCHTVSVGTVLGVVEIKIDQKENNTRLRK
                     LQLLFSLLCVGGLILLIVVFSTLITFFIKKPLIKIKKEMIDKIIAKNLSFSLTELTND
                     ELGDLGRNLFEMKESQREVIKKINVVSGEVKKTVEYLQTASTEISEGAKHAASEINSV
                     ATAGEEMSTTSCDIAQNCHRVAEASQLAVKTAIEGGEILKETIGSMGQISDSVSSTAN
                     SVRSLGSRSKQSGAIVGTIEEIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRALA
                     ERTTVATREIGEMINTIQKETQIAVKKMEEGVSVVREGTEKAEQSGRALNDIIGQVSG
                     VNDQINQISTAAEEQTATTREISGNMHRISSFIAEVSKGNAENLVAIMELQAVVKELH
                     DHVVVFKI"
     gene            complement(3713..4552)
                     /gene="rsmA"
                     /locus_tag="VF357_05235"
     CDS             complement(3713..4552)
                     /gene="rsmA"
                     /locus_tag="VF357_05235"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX7523582.1"
                     /translation="MKEGTSHPGKLLAALGLFPRKSLGQNFLTDRNVAGKIVALARSF
                     PPPFLEIGPGLGALTSLLADAGAPVVAVEIDRGLAGHLRGRLVGTSVEILEADFLAVP
                     EQVWRLRFPAGGTVVGNLPYSISSPIVLRLIELRDRFPRAVLMLQREVVDRLCAGPGG
                     KEYGILSVYLGVLAEARKEFVVRRASFHPAPDVDSAVMSVRFVGEYPEALVAALRTVV
                     RAAFAHRRKTLRNAPVPFLPGGAAQWCELLSASGIDPSGRAEEVSPAAYLALARTFAA
                     CMR"
     gene            complement(4617..5615)
                     /gene="tsaD"
                     /locus_tag="VF357_05240"
     CDS             complement(4617..5615)
                     /gene="tsaD"
                     /locus_tag="VF357_05240"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX7523583.1"
                     /translation="MRVLGIESSCDETAAAVYDVSAGLLSNVVSSQVAVHGAYGGVVP
                     ELASREHLKSIVPVVEKALSDASTGRDAIDGIAVTAGPGLIGSLLVGLCFAKSLALAW
                     GKPLYGADHLEAHVYAVFLESDVPFPYIALLVSGGHTSLFRVEGWGEMKFLGGTRDDA
                     AGEAFDKAAKMMGLPYPGGVAVDRTGREGDSSRFHFPRAWLSRDSADFSFSGLKTALR
                     TFLASPEGKAARKEDVAASFQEAVADVLVGKALSTAERERVPRLVLAGGVSANSRLRS
                     LAQHRGAAAGIATFLPSKALCTDNAAMVALLGERRLTAGVFSGPELSAYAASRFTR"
     gene            complement(5615..6937)
                     /locus_tag="VF357_05245"
     CDS             complement(5615..6937)
                     /locus_tag="VF357_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7523584.1"
                     /translation="MIKNFVLDTNVLLHDPNALFHFEDNRVIVPITVIEELDHFKKDL
                     NMLGRNARTVSKHIDRLRTSGNLGEGVALEHGGVLRVALGSEGASLLPPELEGRAGDN
                     QILGVALSARAREKDIPVVVVTKDTNLRIKADALGITAEDYESDRVDIEELYRGFREV
                     NVGKEFIDAFYAAGECLPPSDARELHPNEYLIFRDAASQSSALGRADALRRKIRLLSP
                     SKDDVWGVRPRNKEQTFALDILLDDSVKLVTLAGKAGTGKTLLAIAAGLRKTSDEEVY
                     QRRLVSRPVFPMGKDLGFLPGDVEEKLKPWMQPIFDNVEYLFGFNRKKRQGAVRGYQE
                     LMNLGILEIEPLTYIRGRSIPNQFIIVDEAQNLSPHEVKTILTRAGENSKVVLTGDPY
                     QIDNPFVDSASNGLSCTVEKFKDEAIAGHVLLVKGERSPLAELAANIL"
     gene            complement(6952..7791)
                     /gene="ybgF"
                     /locus_tag="VF357_05250"
     CDS             complement(6952..7791)
                     /gene="ybgF"
                     /locus_tag="VF357_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX7523585.1"
                     /translation="MARNAVVFPLIGAFAAFMAGCAVVDTGAFTRLQNEMVGLKKEMA
                     VLKTAPSPAPSVRADAGEVQSVRKSFADLNSDFDRMKSDQLAATTRMDDARVEMQRIT
                     ARQDEQDRALQGIRGNADRMKEIENRLAALEERIGKLAAAPAAAPASESPREWRSPEE
                     MYEVAVGQVKGGNPKKGRETLADFAAKYPNHKLIPNALYWKGEAFYAEKDFENAILTF
                     QDVVDKYPRGEKAPDAMYKQGLSFLSLKDKKNAGILFDLVQKKYPKSKAAEMAKKKLT
                     EIR"
     gene            complement(7963..8376)
                     /gene="pal"
                     /locus_tag="VF357_05255"
     CDS             complement(7963..8376)
                     /gene="pal"
                     /locus_tag="VF357_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX7523586.1"
                     /translation="MTEAPAPGAVSPGTESAAAGVAVTEEKYSQFDDVRFDFNKSEMR
                     EDGRKMCQVVADYMKKHPQAKMQIEGHCDDRGTAEYNLALGERRATAVMTYLGSLGVP
                     KAALSTVSFGKEKPLDPGQNEEAWAKNRRAHFVLK"
     gene            complement(8579..9892)
                     /locus_tag="VF357_05260"
     CDS             complement(8579..9892)
                     /locus_tag="VF357_05260"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523587.1"
                     /translation="MKRSIGAAAGAFLLAFSLFPSTAFPVLYIDINAPGGKRMPIAVA
                     DVVVGRDDPSLSRGIPKILSDDLILTDLFDLVPPTAHIETVTGAHFSGKPLSFAGWKM
                     IGAEAVVIGLAEVRGDRLSIEMRLYDATQGTLLLGKRYTGTPTQLRSIVHRFANEIVY
                     TFTGVRGVFATEIAFTARSGKSRGKELYIVGMDGKELRKVTDNRSFNLFPRWSPDGQW
                     LAYTSFRTGLPIVYLRNVSTGAEKEVVRLGGSKAPGGFSPDGAWLYAGVSQAGNSDIF
                     RVRVIGGAVEKVVEGWGLDVSPSPSPDGRRIAFVSDRGGSPQIYVKTIGVAGETRISN
                     AAGYATSPSWSPAGDRIAYTVRSGGRFIVATIAPDGSGFREVASATDGDCEDPSFSPD
                     GRSLVYTYRKRGYSALKIISSDGRRQRTLVSGLEDVGSPAWSPGR"
     gene            complement(9889..10797)
                     /locus_tag="VF357_05265"
     CDS             complement(9889..10797)
                     /locus_tag="VF357_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7523588.1"
                     /translation="MAWSPNRSSGKGPTGSPFFTELSPPPLRTMLALSAVVHMCLAIA
                     ALTVPPLLGTPLRLEPVAVVDLIGGGEFREETRKPPEPTPAASESEKPAPKGRTASTP
                     PPAARGKAAKKAVSNGFVSSKRQATDAASLTDRLRKMREARAGSESVRDAVEERRSEA
                     AARAAVRSVGERVAHRIEAPKPARSVGRFAAGAGGGAQGTVRLSPELLDYFRRLEESV
                     RSSWVLPGALVRDAAKLVVELRIVIEKDGRVSEERIERGSGNPYFDDSVRRAIRKASP
                     LPVPPEQLRGGEDHYEVGFRFHGGAG"
     gene            complement(10766..11188)
                     /gene="tolR"
                     /locus_tag="VF357_05270"
     CDS             complement(10766..11188)
                     /gene="tolR"
                     /locus_tag="VF357_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEX7523589.1"
                     /translation="MAMMSSSGRAGDRRVMAEINVTPLVDVMLVLLIIFMVTAPMLTQ
                     GVDVNLPQASAKALRSDEERLIITVDANSRVFVGKQPVEFNRLSGVLQEIVARRTDRQ
                     VYFRADRSVPYGFVVKVIAEVRNSGIEKLGMVTEPLER"
     gene            complement(11193..11945)
                     /gene="tolQ"
                     /locus_tag="VF357_05275"
     CDS             complement(11193..11945)
                     /gene="tolQ"
                     /locus_tag="VF357_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX7523590.1"
                     /translation="MIVPVELVLPVIPLTAGVFDTQVLKHVMLAGPIVKLVLLLLIGF
                     SVVSWAIIFLKVRFFKGIERNQTGFARAFAEGKSLSALYEQAEKKEKSPLTEVFRAGY
                     LELTRIQRERGEAARGEASQGGRTGPFPVENVYRAMYKTTHEQVGGMENLLPFLATTG
                     SATPFIGLFGTVWGIMNAFSGIATTGNASLATVAPGIAEALVATAAGLGAAIPSVIAY
                     NYFLTRIRTINTRIDSFTAEFINFLERKVDKG"
     gene            12077..12502
                     /locus_tag="VF357_05280"
     CDS             12077..12502
                     /locus_tag="VF357_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX7523591.1"
                     /translation="MKKTRNTRQRGIILDILRESHEHPTADTVYREARRVLPNISLGT
                     VYRNLNFLRDQGTVREIRPSEGGSSRFDGAETPHAHFHCVHCSALLDIPLPPALESLR
                     FEEEERISAVSLVDLHVIGSCCGCAPATGAGHSASSSPA"
     gene            complement(12587..>12862)
                     /locus_tag="VF357_05285"
     CDS             complement(12587..>12862)
                     /locus_tag="VF357_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX7523592.1"
                     /translation="MIYKVAPDILREHGKAKNPWPNVDAQSGVIQWYYGLTEYDFYTV
                     LFGVGRALGVLANITWDRALGYALERPKSVTTDMLEKWAAEGGRKLA"
BASE COUNT         2315 a         4212 c         3782 g         2553 t
ORIGIN      
        1 agcaaccggt ccccttcgcg acccgcacca ggctcacatg cccctcgtgc aggtacccca
       61 tcgtcggcac caggccgatc cgggcccctt ccgcccgccg cgcgtcggcc catgcccgca
      121 tcgccgccgg tgtgtgaagc cgctccatca gaaggagtgc tcctttcccg ggaacgaccc
      181 gtcgcgcacc gcggcggcgt acgccccgac cgcatcctcc accggcttgc gcaattcgcc
      241 gaagcgcttc acgaacttcg gccggaactc gtcgtacagc cccagcaggt cgtgcatcac
      301 cagcacctgc ccgtcgcagc cgacgcccgc gccgatcccg atcgtcggga tcgatagcgt
      361 ccgtgtgatc tccgcggcca gcgcagcggg aatcccctcg agcaccacgg agaacgcccc
      421 cgcctcctgc accgccgccg catcgtccag tagccgttcc cgctgcggat cggtcttccc
      481 ctgcacgcgg tagcctccca tccggtgaac cgactgcggg gtgagcccga tgtgccccat
      541 cacggggatg tccgccgccg cgatcgcccg gatcgtttcc gcggcgatgc gccccccctc
      601 cagcttcacc gcctccgccc ccgcctggat cagccgcccg gcctttcgga tcgcgtcggc
      661 gacgcacgcc tggtacgaca ggaacggcat gtccgccgcg aggaacgcgc gcttgcgccc
      721 ccgcgcggcc gcctccgtgt gccgcaccat gtcctccatg gtcacgttca acgtgtcggg
      781 gtggcccagt accaccatcc ccagcgaatc gccgacgagg atcacgtcca cgccggcgtc
      841 gtcgaagatg cgggcgaaca gggcgtcgta cgccgtgatc atcacgattt tcgccccttc
      901 cgccttcatc cgcgcaaggt cgaggatgtt ggtcttttgc atcgcgcccc gcccccttat
      961 ttgccccaaa gaaaagcggc ctcccggaac cggatccgga aggccgcgtg tcgacacgca
     1021 tcgggcgccg gcagagcgga aacctcccgc accgccgccg ctcccttcta cgatcccgtc
     1081 tcggtccgaa ctacctggat cccagcggta tgtaatgctg cgtcccgcgt ttcacactct
     1141 tgacttctct cacgagatgt tcaaggtcgc gggggctctc cacaaagtcg atctcgctcg
     1201 tgttgaccac caggagcggg gtctcgttat agtggaaaaa gtattcgtta taagcatcgc
     1261 tcagtgtacg caggtagtca agggagatgt ttcgttcgta ggcgatcccc cgcttgcgga
     1321 ctctggacag cagcacctcg ggacgcgcct ggaggtagat gaccaggtcc ggcttgggaa
     1381 gcgtggagac gagcagcttg tagatcgact cgtagaggga gacctcgtcg tcctcgaggt
     1441 ttatgagggc gaagatcttg tccttggcga ggatgtagtc gcacaccagg ccggcctcga
     1501 agaggtcccc ctgggccagt tccctttgct gccgatagcg cgacaggagg aagaagagct
     1561 gggtctggaa ggcgaatttg cgggggtttt cgtagaagcg ctcgaggaac gggttccctg
     1621 cgatctcttc cttgacgagg cgggaggcgt atttctgggc gaggatcttc gccagggacg
     1681 acttcccgac gccgatcggt ccttcgatcg cgatatagcg gggggtcttc ggttcggtca
     1741 tggccggtca attgtatgcg cggagcggcc gattttccac tatattcggg caggtcattt
     1801 cgcacccgtg cccccggcgc ccgcgagatc aatgaaccat tttttcgatg gcctcgcgga
     1861 cagaggattc ctcatgtggt acctaacggt acatagagga caggtctttc ttccccagcg
     1921 cgtgcgcaac gagcagaggg tctttgtttg catttgtgga ggagcattga cttccccctc
     1981 cttctctaaa ttaaatttta aaaacgacta catggtcatg gagttctttt actactgctt
     2041 gaagctccat aattgcaacc agattttccg cattaccttt agaaacctcc gcgataaatg
     2101 aggaaatcct gtgcatattg ccagagattt ctctggtagt ggcagtctgt tcttcagcgg
     2161 cggtagaaat ctgattgatt tgatcattca ccccacttac ttgaccaatt atatcattta
     2221 acgccctgcc agattgttcc gctttttcag ttccttctct tacaacagaa actccttctt
     2281 ccatcttctt aactgctatt tgtgtttctt tctggatagt attaatcatt tctccgatct
     2341 cacgagttgc tactgttgtt ctttccgcaa gagcacgcac ttcatcagct acgacagcaa
     2401 aacctcttcc ttgttctccg gctctggccg cttcgattgc agcattaagt gctaaaagat
     2461 tggtttgatc tgcaatctct tcgatcgtgc caacaattgc accgctctgt ttagaacgtg
     2521 aaccaagaga ccttacggaa tttgctgtag atgaaacact atcggaaatc tggcccatgg
     2581 atcctatggt ttcttttaat atttctccac cctcaattgc tgtttttaca gccagttgcg
     2641 atgcttcagc aacccgatgg cagttttgag cgatgtcaca agacgtagtt gacatttctt
     2701 caccagctgt tgctactgag tttatttcag aagctgcgtg ttttgcaccc tctgaaatct
     2761 ctgtggacgc agtttgaaga tattctactg tttttttaac ctctccggaa acaacattaa
     2821 tctttttaat tacttctctt tgagactctt tcatttcaaa caaatttctt ccaagatcac
     2881 ccaattcatc attcgtcaac tcagtcaaag aaaaagaaag attttttgct ataatcttat
     2941 caatcatttc ttttttaatc tttatcagtg gcttttttat aaaaaatgta ataagcgtag
     3001 agaaaaccac aatcaacaaa attaatcctc caacacacaa taaagaaaat aacaattgta
     3061 gttttcttaa tcttgtgttg ttctcttttt gatcgatttt tatttcaacc acaccaagaa
     3121 cagttccaac ggaaaccgta tgacaatcta aacactcttc tttcgcgata taagggaaaa
     3181 ttcccttaat tgattcagaa gaattgatta tcttttttac ttttctttcc cttactgttt
     3241 tctggttttc atcttcattg ttgtttccat caaatacttc ctttgatggg ataattttga
     3301 tatttccatc gtttttatcc gttatatctt ttatcccttt ttttattgaa tctccttcac
     3361 tgtttttcat aacagcattt attgcagtga acattaagta ttgtgtcttc tctaaactgg
     3421 ttacagcatc ttcctcatat gatttcttta gagaataatt aataataaac atcattactg
     3481 ctattccaac gatcaaaatt gctactaatg gcacggaaag tttcgaggca attgttttca
     3541 ttcgtatttc tgcttgtccg tctccatcta ctttcttcca aaggtatctg agaagtcaac
     3601 cctttttgaa ccttgccttg gtccttcgac cgggagcgct gcttccggga gaggaaccca
     3661 aattccggag aagtgtcgtt cgatctgcgg tggggaagtc gggaagtatt cgtcatctca
     3721 tgcaggctgc gaacgtccgc gccagcgcga ggtacgcggc gggcgatacc tcctccgccc
     3781 ggccggaggg gtcgatcccc gacgccgaaa gcagctcgca ccattgcgcc gcccccccgg
     3841 gcaggaacgg gacgggggca ttgcgcagcg tcttccgtcg gtgcgcgaag gcggcgcgga
     3901 cgaccgtccg caacgccgcg accagggcct cgggatattc tccgacgaaa cggaccgaca
     3961 tcactgccga gtcgacgtcc ggtgcgggat ggaagcttgc gcgccgcacg acgaactcct
     4021 tgcgcgcctc cgcgaggacg ccgaggtaga ccgagaggat cccgtactcc ttccctcccg
     4081 gaccggcgca caggcggtcc accacctccc gctgcagcat caggaccgcc cgggggaaac
     4141 gatcccgcag ttcgatcagc cggaggacga tgggggagga gatggagtac gggagattcc
     4201 cgaccaccgt acctcccgcc gggaagcgca atcgccacac ctgctccggc acggcgagga
     4261 agtccgcttc gagaatctcc acgctcgtgc ctacgagcct gccccgaagg tgccccgcca
     4321 ggccgcgatc gatctcgacc gccacgacgg gcgctcccgc atcggcgagc agtgaagtca
     4381 gcgccccgag cccggggccg atctccagga acggcggcgg gaaagagcgc gccagtgcga
     4441 cgatcttgcc ggccacgttc cggtccgtga ggaagttctg cccgagcgac ttgcggggga
     4501 acaggccgag ggcggcgagg agcttccccg gatgcgatgt gccctctttc actctacggt
     4561 ccacatttta tgtaggcgcc gctccgcggg ataccccttc cgggcgggtt ggccacttag
     4621 cgggtaaagc gggaggccgc ataggcgctc agctccggcc cggagaagac ccccgccgtg
     4681 agacgccgct cgccgagcag cgccaccatc gccgcgttgt cggtgcatag cgccttcgaa
     4741 gggagaaacg tcgcgatccc cgccgcggcg cccctatgct gcgccagcga acgcaggcgg
     4801 gagttcgccg agacgccgcc ggccagcacc agccgcggaa cccgctcgcg ctccgcggtg
     4861 gagagcgcct tccctacaag tacgtcggcg accgcttcct ggaaggaggc ggcgacgtct
     4921 tccttccggg cggccttccc ttccggggag gcgaggaagg tccgcagcgc cgtcttgagg
     4981 cccgagaagg agaagtccgc cgagtcgcgg gagagccagg cccgcgggaa gtggaaccgc
     5041 gaagaatctc cctcccggcc ggtccgatcg accgcgaccc cgccgggata cggaagcccc
     5101 atcatcttcg ccgccttgtc gaacgcctcc cccgccgcgt cgtcccgcgt tccgccaagg
     5161 aatttcatct ccccccaccc ttcgacgcgg aacagggacg tgtggccgcc ggacaccagc
     5221 agcgcgatgt agggaaaggg aacgtcgctc tcgaggaaga cggcgtagac gtgcgcttca
     5281 aggtgatccg cgccgtagag cggttttccc cacgcgaggg cgagcgactt cgcgaagcac
     5341 aggcccacga ggagcgatcc gatgagcccc ggcccggcgg taaccgcgat cccgtcgatc
     5401 gcgtcccgcc cggtggaggc gtcggacagc gccttctcga ccaccggaac gatcgatttc
     5461 agatgttcgc gggaggcgag ctcgggaacc acgccgccgt aagcgccgtg gaccgcgact
     5521 tgggacgaaa cgacgttgga aagcaacccc gcggagacgt cgtagacggc ggcggcggtc
     5581 tcgtcgcagg agctttcgat gccgaggaca cgcactacag gatgttcgcg gcgagctccg
     5641 cgagcgggga acgctccccc ttgacgagaa gtacgtgccc cgcgatcgcc tcgtccttga
     5701 acttctccac cgtgcaggaa agaccgttgc tcgccgaatc gacgaagggg ttgtcgatct
     5761 ggtacgggtc gcccgtcagg accaccttgc tgttctcccc ggcgcgggtg aggatcgtct
     5821 tcacctcgtg cggagacagg ttctgcgcct cgtccacgat gatgaactgg ttcgggatgg
     5881 agcggcctcg aatgtaggtg agcggctcga tctcgaggat ccccaggttc atcagctcct
     5941 ggtacccgcg cacggccccc tgccgcttct tccggttgaa tccgaacagg tattccacgt
     6001 tgtcgaagat cggctgcatc cagggcttga gcttctcctc cacgtctccc gggaggaacc
     6061 cgaggtcctt ccccatcgga aagacgggac gggatacgag cctccgctgg tacacctcct
     6121 cgtccgaggt tttccgcaac ccggccgcga tcgccagcag ggtcttcccg gttccggcct
     6181 tgccggcgag ggtcacaagc ttgacggagt cgtcgaggag gatgtcgagg gcgaaggtct
     6241 gctccttgtt ccgcggcctg acgccccaga cgtcgtcctt cgacggggag agaaggcgga
     6301 ttttcctgcg cagcgcgtcg gcccgtccca gcgccgacga ctgggaggcg gcgtcgcgga
     6361 agatgaggta ctcgttcggg tgcagctccc gggcgtccga aggcggaagg cactcccccg
     6421 ccgcgtagaa ggcgtcgatg aactccttgc cgacgttcac ctcccggaac ccgcggtaca
     6481 gctcctcgat gtcgacccgg tcgctctcgt aatcctcggc cgtgatcccg agggcgtccg
     6541 ccttgatccg caggttcgta tccttggtga ccacgaccac gggaatgtcc ttttcccgcg
     6601 cccgcgccga aagggcgacc ccgaggatct ggttgtcccc ggcccggcct tccaattccg
     6661 gtggaagcag ggatgccccc tcgcttccga gcgccacgcg tagaacccct ccgtgctcca
     6721 gcgcaacccc ctccccgagg ttgccggagg tgcgcagccg gtcgatgtgc ttggacaccg
     6781 tccgggcgtt gcgcccgagc atgttcaggt ccttcttgaa atgatcgagt tcctcgatca
     6841 ccgtgatcgg gacgatcacc cggttgtctt cgaaatggaa cagcgcattg ggatcgtgca
     6901 gaaggacgtt cgtgtccagc acgaaattct tgatcacgtg ggcggaggct cctaccggat
     6961 ctccgtgagc ttcttcttcg ccatctcggc cgccttcgac ttcgggtact tcttctggac
     7021 gagatcgaac aggatcccgg cgttcttctt gtctttcagc gacaggaacg acaacccctg
     7081 cttgtacatc gcgtcgggcg ccttttcccc ccgggggtat ttgtccacca cgtcctggaa
     7141 cgtgaggatg gcgttctcga aatccttctc cgcatagaac gcctcgccct tccagtagag
     7201 cgcgttcggg atcagcttgt ggttcgggta tttcgccgcg aagtcggcca gcgtctccct
     7261 ccccttcttc gggttcccgc ccttcacctg tcctacggcg acctcgtaca tctcctccgg
     7321 gcttctccat tcccgcgggc tttccgaagc gggcgccgcg gccggagcgg cggcgagctt
     7381 gccgatccgc tcctcgagag ccgccaggcg attctcgatc tctttcatcc ggtcggcgtt
     7441 cccgcggatc ccctgcagcg cccggtcctg ctcgtcctgc cgggcggtga tccgctgcat
     7501 ctctacccgc gcgtcgtcca tccgggtggt ggcggccagc tggtccgact tcatccggtc
     7561 gaaatcggag ttcaagtccg cgaagctctt ccgcacggac tggacctcgc ccgcgtcggc
     7621 gcgcaccgag ggagcgggcg agggcgcggt cttgagcacc gccatctcct tcttcagccc
     7681 caccatctcg ttctggagcc gcgtgaaggc gccggtgtcc acgaccgcgc accccgccat
     7741 gaaagcggcg aatgccccga tcagcgggaa aacgaccgcg ttacgcgcca ccccgttcac
     7801 ctgagggtcc gtccttgaat aaggtgtgca gatatgggcg tcgagtcgcc cggccggcaa
     7861 ggcgcgagga aggagaatac cgagagtatt cgaccgacga gcaacgcagc ccgccgggat
     7921 gaatcggcgc ccagatcgca caagttattc atggacggga cctcacttca ggacgaagtg
     7981 cgcgcggcgg ttcttcgccc acgcctcctc gttttgaccc ggatccagcg gcttttcctt
     8041 gccgaagctg accgtggaga gagccgcctt cggaacgccg agggacccga ggtaggtcat
     8101 cacggccgtc gcgcgacgtt cacccagcgc caggttgtac tccgcggtgc cgcggtcgtc
     8161 gcaatgcccc tcgatctgca tcttcgcctg ggggtgcttc ttcatgtagt cggccacgac
     8221 ctggcacatc ttgcggccgt cctctctcat ctcagacttg ttgaagtcga accggacgtc
     8281 gtcgaactga gaatacttct cctcggtgac cgcgacaccg gcggccgcgg actccgttcc
     8341 cggggagacc gcaccgggag ccggcgcctc tgtcacgggc gcttcaccga ccgagggggc
     8401 ggaaaccgct ggcgctccct gagtcccttc ggacttcacc gtctgtttct tggcgcaccc
     8461 taccgctacc agggccgcaa cggacaacac caaagcggcc catttccagg aaaatgtacc
     8521 cttcattctg ttcccctcct tttaatggat gaatccgggc acgttcgcgc cctcaaagct
     8581 accgtccggg tgaccaggcg ggtgaaccga catcctcaag gccggaaacg agcgtccgct
     8641 gtctccgccc gtccgacgag atgattttga gggcagaata gccccttttc ctgtacgtgt
     8701 aaactaggga gcggccgtcg ggggaaaaag acgggtcttc gcagtcgcca tccgtcgcgg
     8761 acgcgacttc ccggaacccg gatccgtcgg gtgcgatggt cgccacgatg aatcgtccac
     8821 cggaccggac ggtgtaggcg atccggtccc ccgcggggga ccaggacggc gacgtggcgt
     8881 agccggccgc gttggagatc cgcgtctctc cggcgacgcc gatcgtcttt acgtatattt
     8941 gcggtgaacc gccgcggtcg gagacgaagg cgatccgccg cccgtcgggg gacggcgagg
     9001 gggagacgtc gagcccccac ccttccacca ccttttccac ggcgcctccg atcacccgta
     9061 cccggaagat gtcggaattg ccggcctggc tcacgcccgc gtagagccac gccccgtcgg
     9121 gcgagaaccc cccgggggcc ttcgagcctc ccagccggac gacctctttc tccgccccgg
     9181 tcgagacgtt gcgcaggtag acgatgggca ggccggttcg gaacgaggtg tacgccagcc
     9241 attgtccatc gggggaccag cggggaaaga ggttgaagct ccggttgtcc gtcaccttgc
     9301 gcagttcctt cccgtccatc cccacgatgt agagttcctt tcccctggac ttccccgagc
     9361 gggcggtgaa cgcgatctcg gtcgcgaaaa cgccccggac cccggtgaag gtgtacacga
     9421 tctcgttggc gaaccggtgc acgatggaac ggagctgggt cggggtgccg gtgtaccgct
     9481 tcccgagcag gagcgttccc tgcgtggcat cgtagagacg catctcgatc gagagccggt
     9541 cgccccgcac ctcggccagc ccgatgacca ccgcctcggc gccgatcatc ttccatccgg
     9601 cgaacgacag cggcttgccg gagaagtgcg cccccgtcac cgtctcgatg tgggcggtcg
     9661 gcgggaccag gtcgaacagg tcggtgagga taaggtcgtc ggagaggatc ttcgggatcc
     9721 cccgggagag ggacggatcg tccctgccga ccacgacgtc cgccaccgcg atcggcatcc
     9781 gcttcccgcc gggggcattg atgtcgatgt agaggacggg gaacgccgtg gagggaaaca
     9841 gggagaacgc gaggaggaac gcacccgccg ccgcgccgat cgatcgcttc accccgcacc
     9901 cccgtggaat cggaatccta cctcgtaatg atcctccccg ccccgcagtt gttccggggg
     9961 aacagggaga gggctcgcct tccggatggc gcgccgcacg gagtcgtcga agtacgggtt
    10021 accggaaccc cgctcgatcc gctcctcgga gacgcggccg tccttctcga tcacgatccg
    10081 cagctccacc acgagtttcg ccgcgtcgcg cacaagcgcc ccggggagga cccagctgct
    10141 ccggacgctc tcctcgagcc gacggaaata gtcgagcagc tccggggaaa ggcgaacggt
    10201 tccctgcgcc ccgccgccgg cgcccgcagc gaagcgcccc acggaccgcg cgggcttggg
    10261 agcctcgatc cggtgggcca cgcgctctcc cacgctgcgc acggccgcgc gcgccgccgc
    10321 ctcgctcctg cgctcctcca ccgcatcccg gaccgactcc gatccggcgc gggcctcccg
    10381 catcttgcgc agccggtcgg tcagcgacgc cgcgtcggtg gcctgccgtt tcgacgagac
    10441 gaagccgttg gaaacggcct tcttcgccgc cttcccgcgc gccgccggag gaggcgtgga
    10501 tgccgtccgg cccttgggcg cgggcttctc gctttcgctc gcagccggcg tcggctccgg
    10561 gggcttcctc gtttcctcgc ggaattcccc gccgccgatc aggtccacca cggccaccgg
    10621 ttcaaggcgc agaggagtcc ccagcagagg gggaacggtc agcgccgcga tcgccaggca
    10681 catgtggacc accgccgaga gggcgagcat ggtccgcagc ggcggcgggg agagctccgt
    10741 gaaaaacggc gagcccgtcg gccctttacc gctcgagcgg ttcggtgacc atgccaagtt
    10801 tttcgatccc ggagtttcgg acctccgcga tgaccttcac cacgaacccg taagggacgg
    10861 agcggtcggc gcggaaatac acctgacggt cggtccgccg ggcgacgatc tcctggagga
    10921 cgccggacag gcggttgaac tcgacgggct gcttccccac gaagacgcgg ctgttggcgt
    10981 cgacggtgat gatcaggcgc tcctcgtcgc tgcgcagcgc cttcgcgctc gcctgcggca
    11041 ggttcacgtc gaccccctgc gtgagcatgg gagcggtgac catgaagatg ataaggagaa
    11101 cgagcatcac gtcgaccagc ggcgtgacgt tgatctccgc catgaccctt cgatccccgg
    11161 cgcggccgga agagctcatc atcgccatgg cgtcatccct tgtcgacctt gcgctcgagg
    11221 aagttgatga attcggccgt aaagctgtcg atccgggtat tgatcgtccg gatgcgggtc
    11281 aggaagtagt tgtaggcgat gacggacggg atggccgcgc ccagtcccgc cgcggtggcg
    11341 acgagggcct cggcgatccc gggggccacg gtggccaacg aggcgtttcc cgtggtagcg
    11401 attcctgaga aggcgttcat gatcccccat acggtgccga acaggccgat gaacggcgtg
    11461 gcgctgccgg tggtggcgag aaagggaagg agattttcca tgccccccac ctgctcatgg
    11521 gtcgttttgt acatcgcgcg gtagacgttc tcaacaggaa aggggcccgt gcgtccgccc
    11581 tgcgacgcct cgccgcgggc cgcctcgccc cgctcccgct ggatccgggt cagctcgagg
    11641 tacccggcgc ggaacacctc ggtaaggggc gacttctcct ttttttcggc ctgctcgtaa
    11701 agcgcggaca ggcttttccc ctcggcgaag gcgcgggcga atccggtctg gttccgctcg
    11761 atccccttga agaaccggac cttgaggaaa ataatggccc aggagaccac ggagaacccg
    11821 atcaggagca gcaggacgag cttgacgatg ggccccgcca acatgacgtg cttcaacacc
    11881 tgggtgtcga aaacccccgc cgtcaggggg ataacgggca aaaccagttc cacgggaacg
    11941 atcaaggcat cctcctccgg tcgatctgct tcaacacctc tatataacgg attcgaggcg
    12001 ccgtttcaat catcttgaca atctttttta gtatacatag gatacggttc agtaccacac
    12061 ccataaagga atatagatga agaagacccg gaacacccgg caacgcggca tcatcctcga
    12121 catcctcagg gaatcacacg agcaccccac cgccgatacg gtctatcggg aagcgcgccg
    12181 ggtgctcccc aacatcagcc tggggaccgt ctaccggaac ctgaacttcc tgcgggacca
    12241 gggaaccgtg cgggagatcc ggcccagcga agggggctcc tcccgcttcg acggagcgga
    12301 gactccccac gcgcacttcc attgcgtcca ctgcagcgcg ctgctcgaca tcccgctgcc
    12361 cccggccctc gaaagcctcc ggttcgagga ggaggaaagg atctcggccg tttccctcgt
    12421 cgacctgcac gtcatcggct cctgctgcgg gtgcgccccg gcgactggag caggacactc
    12481 cgcctcttcc tccccggctt gaaccaagcg ggtcccgctg aaccctttca aacggaaagg
    12541 gggcctttcg gccccctttc cgtttccgtg tttccggcgg tgccgcttac gcgagcttgc
    12601 ggcccccttc ggcggcccac ttctccagca tgtcggtcgt gaccgacttc ggacgctcga
    12661 gcgcgtaccc cagcgcgcga tcccaggtga tgttcgccag gacgccgagg gcacggccga
    12721 cgccgaagag cacggtgtag aagtcgtact cggtgaggcc gtagtaccac tggatgacgc
    12781 ccgactgcgc gtcgacgttc ggccacgggt tcttcgcctt cccgtgctcc cggaggatgt
    12841 ccggcgccac cttgtagatc at
//
LOCUS       DASVJL010000256         6236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_134476, whole genome shotgun sequence.
ACCESSION   DASVJL010000256 DASVJL010000000
VERSION     DASVJL010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134476"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VF357_05290"
     CDS             <1..562
                     /locus_tag="VF357_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7523593.1"
                     /translation="TTKERGSGTGLGLATTYGIVKQAGGSIEVYSEVGIGTTFKIYLP
                     RVEEGAEKPVEDDRPTDFSGGTETVLVVEDEDRVRDLCVRILERLGYKVLRGSNGTEA
                     IAVAQKYGDRIDLLVTDVVMPGMSGAEMAAQLVLHHPEMKVLFTSGYTDGAIVRHGVL
                     EEGVSFIGKPYTPSALAKKVREVLGR"
     regulatory      660..762
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            809..1009
                     /gene="thiS"
                     /locus_tag="VF357_05295"
     CDS             809..1009
                     /gene="thiS"
                     /locus_tag="VF357_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX7523594.1"
                     /translation="MKLIVNGEAYEAKQPESVMALLEEMGVGGRAVVVVLNDEVIPTS
                     SRSTCGLREGDCVELFRFVGGG"
     gene            1029..1862
                     /locus_tag="VF357_05300"
     CDS             1029..1862
                     /locus_tag="VF357_05300"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX7523595.1"
                     /translation="MKRGSGGEGMERPGKVQDEWIVGGHRFSSRLLMGTGKFQDAETM
                     VGAVLASGTQIVTVALRRVGRIPQEDDMLGPLQQLKGITLMPNTSGARNASEAIRAAR
                     LGRELCGSPFVKVEIHPNPHHLMPDPIETFEAARILAAEGFVVMPYMPADPVLAKRLE
                     DVGCASLMPLGSAIGSGRGLATAEMLRLILRDARIPVIVDAGLRSPSEAAAALEMGCD
                     AVLVNSAVAASGDPIAMAAAFAQAVGAGRSARQAGLMPQGGGTALATSPLTAFLSVEG
                     P"
     gene            1859..2989
                     /gene="thiH"
                     /locus_tag="VF357_05305"
     CDS             1859..2989
                     /gene="thiH"
                     /locus_tag="VF357_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HEX7523596.1"
                     /translation="MTPQMPAWLEPSPWLDGRGSTPADVERALGCDEPGLRELAALLS
                     PAAHSFMEVMALRARDLTQRHFGRTISMYAPLYLANYCTNGCAYCGFASDRVQPRRRL
                     EPPEAEKELVMLKEMGFEEVLLLTGERTSHAGFDYLLECVSLAAQRFHAVGVEAFPMT
                     TREYALLAEAGCSGVTLYQETYDPRQYDRLHRWGPKKDYLDRLDAPARAMEAGIRTVG
                     LGVLLGLSDPAFDAVCLLRHADHLRRNHWKSGITLSFPRLRPQAGNFTAMFPVDEAWL
                     AQLVFAVRIVLPEVPLLLSTRERPEFRDGMAGLGISKMSAGSRTSVGGYGEAEPATEG
                     QFEVSDKRSVPDFCAMLRNRGLAPVFKNWDSVFRDAEAGEHG"
     gene            2982..3626
                     /gene="thiE"
                     /locus_tag="VF357_05310"
     CDS             2982..3626
                     /gene="thiE"
                     /locus_tag="VF357_05310"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX7523597.1"
                     /translation="MGDAFGLYLVMTDPVVGYEACAAAAVRCGVRYLQLRMKGAPRHA
                     VLETALRIREIALGSDTLFIVNDDVTIARDVDADGVHLGQRDLPIDEARRLWPAPGKR
                     FGLSTHDEQQALLASRLSPDYIGVGPVFATPTKAIPDPVLGLERMASIIRSAPVEAVA
                     LGGIDCRNLAEVLRRGARNFCVVRAVNRRPDPETAIRELQGIWRQERGCGIGLS"
     gene            complement(3694..4554)
                     /locus_tag="VF357_05315"
     CDS             complement(3694..4554)
                     /locus_tag="VF357_05315"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX7523598.1"
                     /translation="MVEDKSAPWYATLTREYRFTSTVPVLRLLIQQSNCFIVVERGQG
                     MRAMQEERALQDSGELRSGSSFGKGQMVSADYGIKPEVLFSAKDAGGMGGALRGQSGA
                     LGVAGSVLGSVKTREASTMLLLVDNRSGVQVAAAEGSSSKMDIGLSTSLFGAHGGGGL
                     GGYTNTPEGKVIAAAFTDSYNQMVRSLRNYTPQSMGGQGLGTGGKLTVEGAQQPAAVA
                     PAGSAPPAARTMRLADAQSKLASLGYDPGPADGAMGKRTVNALRAFQRDRNLPISGRL
                     DAATIAELSK"
     gene            5002..5760
                     /gene="ygiD"
                     /locus_tag="VF357_05320"
     CDS             5002..5760
                     /gene="ygiD"
                     /locus_tag="VF357_05320"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEX7523599.1"
                     /translation="MPVLFVGHGSPTNAIEENEFSRVWADAGKAMPRPGAVLCVSAHW
                     ETNGTQVTAAERPGTIHDFYGFPRPLYEVSYPAPGAPELARRVRDTVRGARVRLDPDR
                     GLDHGAWSVLRRMFPEADIPVVQLSLDRAKGPAFHYELGKELAGLRREGVLIVGSGNI
                     VHNLGVIVREDTAYDWAVEFDETVKRLILSGDHDAIVHSPDLGRAARLSVPTNEHFLP
                     LLYVLALQEKGEGIRFFADRVTLGSISMRSLWIG"
     gene            complement(5746..5928)
                     /locus_tag="VF357_05325"
     CDS             complement(5746..5928)
                     /locus_tag="VF357_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523600.1"
                     /translation="MTTPISYIRKTAIKHKVPYITTTAAAIATAKGISAHRQGHADVR
                     SLQQYHAGIGGGSPDP"
BASE COUNT         1036 a         1915 c         2188 g         1097 t
ORIGIN      
        1 caccacgaag gagcggggga gcggaacggg tctggggctt gcgacgacct acggcatagt
       61 gaagcaggcg ggtggctcca tcgaggtgta ctcggaggtc gggatcggga cgaccttcaa
      121 aatctatctg ccgcgtgtcg aggagggggc cgagaaaccg gtggaagacg accggccgac
      181 ggacttttcg ggcgggacgg agacggtgct ggtggtggag gacgaggaca gagtgcggga
      241 cctgtgcgtc cggatattgg agcggctggg atacaaggta ctgcgggggt cgaacggcac
      301 cgaggcgatc gcggtggcgc aaaagtacgg cgaccggatc gaccttctcg tgacggacgt
      361 tgtgatgcct gggatgagcg gggccgaaat ggcggcgcaa ctggtcctgc accacccgga
      421 gatgaaggtt ctgttcacat ccgggtacac ggacggcgcg atcgtgcgcc acggggttct
      481 ggaggaaggc gtgtcgttta tcgggaagcc gtacaccccg tcggcattgg cgaagaaggt
      541 tcgggaagtg ctcggcaggt agtaaacgtc tggagggggc cgcgccgagt ggcaaaaagt
      601 ggctcgcagc ccggagtagg aatatgggtt gacggacacg gacgggccac gtattattcg
      661 ggcagtagcc ggggtgctcg tcccgaacgg ggcgggttga gatcataccc gttgaacctg
      721 atccggataa tgccggcgca gggaagccaa cgagatgcgc gcctggcgga tgccttggcg
      781 cgtgtttttt ttatcgaagg agatgggcgt gaaactgatc gtcaacggtg aggcgtacga
      841 agcgaaacag cccgaatccg tgatggcgct gcttgaggag atgggcgtcg gcggacgggc
      901 ggtggtcgtc gtcctgaacg atgaagtgat ccccacctcc tcccggagca cgtgcgggct
      961 gcgggaaggt gattgcgtgg agcttttccg cttcgtcggc ggcggttgat cggagggatg
     1021 gagaaggcat gaagcgggga tccggagggg aaggcatgga gcggccaggc aaggtacagg
     1081 acgagtggat cgtcgggggg caccggttct cctcgcggct cctgatgggg accggcaagt
     1141 tccaggatgc ggaaaccatg gtgggggccg tcctggcatc gggtacgcag atcgttacgg
     1201 tggcgctccg ccgcgtcggc cgtattcccc aggaagacga catgctcggt ccgcttcagc
     1261 agcttaaggg gatcaccctg atgccgaaca cctccggagc gcggaacgcg tcggaggcga
     1321 tccgcgcggc acgtctgggg cgggagctct gcgggagtcc cttcgtgaag gtcgagatcc
     1381 accccaaccc gcaccatctc atgcccgacc cgatcgagac gttcgaggcc gcaaggatcc
     1441 tcgccgcgga agggttcgtg gtgatgccgt acatgccggc cgacccggtc ctcgccaagc
     1501 ggttggagga cgtcgggtgc gcgtcgctga tgccgctggg ttccgcgatc ggaagcgggc
     1561 gcgggctggc cacggccgag atgctgcggc tcatccttcg tgacgcccga atccccgtga
     1621 tcgtcgacgc gggtctccgt tccccttccg aagctgccgc cgcgctcgag atggggtgcg
     1681 acgcggtcct cgtgaacagc gccgtggcgg cgtccggcga cccgatcgcc atggccgcgg
     1741 cgttcgcgca ggccgtgggg gcgggccgca gcgcgcgcca ggccggtctc atgccgcaag
     1801 gcggcggcac ggctctggcg acaagccccc tgacggcgtt cctttccgtg gagggcccgt
     1861 gactccgcaa atgcccgcgt ggctggaacc ttcgccgtgg ctcgacggaa gggggtcgac
     1921 gcctgcggac gtcgaacggg cgctcgggtg cgatgagccc ggcttgcggg aacttgccgc
     1981 gctgctttcc ccggcggcgc actccttcat ggaggtcatg gcgctgcgcg cccgcgacct
     2041 gacgcaacgc catttcggcc gcaccatctc catgtacgcg ccgctctacc tggcgaacta
     2101 ctgcacgaac ggctgcgcgt actgcgggtt tgcctccgac cgggtccagc ccaggcgtcg
     2161 ccttgaacct ccggaggcgg agaaggaact ggtgatgctg aaggagatgg gattcgagga
     2221 ggttctcctg ctcaccgggg agcgcacctc ccatgccggc ttcgactacc ttctcgaatg
     2281 cgtgtcgctg gcggcgcaac ggttccatgc cgtcggggta gaggcctttc ccatgacaac
     2341 ccgcgagtat gccctccttg ccgaagcggg ttgttccggc gtgacgttat accaggagac
     2401 gtacgatccg cgccagtacg accgactcca ccggtggggc ccgaagaagg actacctgga
     2461 tcgtctggat gcgccggccc gcgccatgga agcggggatc aggaccgtcg gcctgggggt
     2521 tctcctcgga ctgtcggatc cggccttcga cgccgtctgc ctcctgcgcc atgcggacca
     2581 cctgcggcgg aatcactgga agtcggggat cacgctttcg ttccctcgcc tgcggcccca
     2641 ggcggggaat ttcacggcga tgttccccgt cgacgaggcg tggctggcgc agttggtgtt
     2701 cgcggtccgg atcgttctcc cggaggtccc tctcctgctc tcgacgcggg aacggccgga
     2761 attccgggac ggcatggccg ggctcggcat ctcgaagatg agcgctggaa gccgcacgag
     2821 cgtcggcggc tacggcgagg cggagccggc gaccgaaggc cagttcgagg tcagcgacaa
     2881 gcgcagcgtg ccggattttt gcgcgatgct gcggaaccgg gggctggcgc cggtcttcaa
     2941 gaactgggac agcgtgttcc gggacgcgga ggcgggcgag catgggtgat gcgttcggcc
     3001 tttacctcgt catgaccgac ccggtcgtcg gctacgaggc gtgcgcggcg gcggcggtcc
     3061 gttgcggcgt acgctatctc cagctccgga tgaagggggc gcctcggcac gccgttctcg
     3121 aaaccgccct gcgcatacgc gagatcgccc tcggcagcga taccttgttc atcgtcaacg
     3181 acgacgtgac gatcgcccgc gatgtggacg ccgacggagt gcacctcgga cagcgcgacc
     3241 ttccgatcga cgaggcgcgg cgcctgtggc cggcgccggg caagcgtttc ggtctttcga
     3301 cccacgatga acagcaggcg ctccttgcat cgcgcctgtc acccgactac atcggggtcg
     3361 gtccggtgtt cgccaccccg accaaggcga ttcccgaccc ggtcctcggc ctcgaacgga
     3421 tggcgtcgat catccgatcg gccccggtgg aggcagtcgc ccttggcggg atcgactgcc
     3481 gaaacctcgc ggaggtgctc cgccgcggcg cccggaactt ctgcgtcgta agggcggtca
     3541 accggcgacc ggatccggaa accgcgattc gcgagctgca gggcatctgg cggcaggaaa
     3601 ggggatgcgg aatcgggttg tcatgagggg acgcagatgg aaagggccgc ttgctctgcg
     3661 gcccttgcgt gcggcagaaa atgttggtgg cggctacttc gacaattccg cgatcgtcgc
     3721 tgcatccagc cgcccggaaa tcggcagatt ccggtctctc tgaaaggcac gcagggcgtt
     3781 caccgtccgc ttgcccatcg ccccatccgc cgggcccggg tcatagccca gcgaggcgag
     3841 cttgctttga gcgtcggcaa gacgcatcgt gcgtgcggcg gggggggcgg atccagccgg
     3901 cgcaacggcg gccggttgct gagccccttc gaccgtcagc tttcctccgg tccccagacc
     3961 ctgcccgccc atggactgcg gcgtatagtt gcgcagcgac cgcaccatct ggttgtaaga
     4021 gtcggtaaac gccgccgcga tgactttgcc ctccggtgta ttcgtgtagc cgcccaggcc
     4081 accacctccg tgcgcgccga acaacgaggt ggagagcccg atatccatct tcgacgagct
     4141 cccctcggcg gccgcaacct gaactccgga ccggttgtcg acgagtagca gcatcgtgga
     4201 tgcttctcga gttttcaccg agccgaggac cgagccggcg acgcccagcg ccccgctctg
     4261 ccccctcaac gccccgccca ttccgccggc atccttggcg ctgaacagaa cctcgggctt
     4321 gatgccgtag tcggccgaca ccatctgtcc cttcccgaaa ctgctgccgg agcgcaattc
     4381 ccccgaatcc tgcaacgcgc gctcctcttg catggcgcgc atgccctggc cgcgctcgac
     4441 cacgatgaaa caattggact gctggatcag caggcgaagc acgggaaccg tcgaggtgaa
     4501 ccggtattcg cgggtcagcg tggcgtacca tggcgcagac ttatcctcga ccaccgcgac
     4561 ggtcccgaaa ggccggtcac atttctcgag ctcgggattt gctccgggac gtggctgccc
     4621 ctgccgctga tcccgtcgcg gtcgtcttgg cgtccttgga gcccatctgc atggaggttg
     4681 ccgcacaacc ggccaaagcg actgcgatga acaggggcag aaaaaaggct tgcggacgcg
     4741 ccattctcat ctcatcttct gctggttccg gtgggcgatg ctaccccatt gaaaaattcg
     4801 gcatttgaca acaatgctta catcgcacgt ccgtcgtggt caaggaaaaa cccgtcaagg
     4861 acctcatggc gatcgccggc ttcttcgaat ggggagggga aggcggatac cgcggtatca
     4921 tgtaaagata tccggacggg tgagattcga cgaggtccag ggaaggagca caacacggcg
     4981 aaaatcgttt ccgatgtcaa gatgccggtc ctgttcgtgg ggcacggcag cccgaccaat
     5041 gcgatcgagg agaacgagtt cagccgggtg tgggccgatg cgggcaaggc catgccgcgg
     5101 cccggggccg ttctgtgcgt ctccgcgcac tgggagacga acggcacgca ggtcaccgcc
     5161 gcggaacggc ccggcacgat ccacgacttt tacggattcc cgcggccgct gtacgaggtg
     5221 agctatcccg cccccggcgc cccggaactg gcgcgtaggg tgcgggatac cgtccggggg
     5281 gcgagggtgc ggctcgatcc cgaccgcggg ctggatcacg gcgcatggtc ggtcctgcgc
     5341 cggatgtttc cggaggccga tatcccggtg gtccagttaa gcctggatcg cgcgaagggc
     5401 cccgcattcc attacgaact gggaaaggag ttggcgggcc tccggcgcga gggagtcctg
     5461 atcgtcggca gcgggaacat cgttcataac ctcggggtta tcgtccggga ggacacggcc
     5521 tacgattggg cggtcgagtt cgacgagacg gtgaagcggt tgatcctgtc gggggaccac
     5581 gacgcgatcg tgcattctcc ggatctcggg cgggcggcgc ggttatcggt gccgacgaac
     5641 gaacatttcc tgccgctgct ttacgttctc gccctccagg agaaggggga ggggatccgg
     5701 ttttttgcgg acagggtgac gctgggatcc atctccatgc ggtcgctatg gatcgggtga
     5761 tccgccgccg atccccgcgt ggtactgctg gaggcttcgg acgtcggcat gcccctgccg
     5821 gtgcgcggag atccccttgg ctgtggcgat cgcggccgcc gtggtcgtga tgtagggaac
     5881 tttgtgcttg atggccgttt tccggatata ggatatagga gtcgtcatgc atgctggtct
     5941 tcccggccgg ggtgttgacg accaggtgga tctccccgtt cttggacgtc gtggacggat
     6001 ggtcgcggga gaagttccct cggcagtaaa cctcacgccg gcgcctttgc caagcggcga
     6061 aacgtccggc gaacaaaacg gggcagggtc gtttccgccc ctgccccgtg gtcgttcccg
     6121 gcgcgaaccg gtcgttgttg cgattattcc ccgaccgggg tgaacgttcc cgccttcgcc
     6181 ttgaacaggg ccttgtggaa ctcccggttg aggcgggcga tgaacttcac gtcgac
//
LOCUS       DASVJL010000257         5837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1166747, whole genome shotgun sequence.
ACCESSION   DASVJL010000257 DASVJL010000000
VERSION     DASVJL010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166747"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..642)
                     /locus_tag="VF357_05330"
     CDS             complement(52..642)
                     /locus_tag="VF357_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523601.1"
                     /translation="MLQFVPPEAELFRGNPALQPRRPTIRYVSPRFQGPAVLAFAAII
                     ATGGAIFWKLVDSEFQRVFLHAAIRGHYTFGSAYDIVRDLLAWHLAGLFVGVFLAGSV
                     LVLLLIAATRHGIGKAVDSLRASADGDLSTPTGTCPIGELDRFGEKIDAARSDTLASV
                     LKIRSEAAALAVGGISPEEFRLRWDELKQQIRRIAP"
     gene            663..1220
                     /locus_tag="VF357_05335"
     CDS             663..1220
                     /locus_tag="VF357_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523602.1"
                     /translation="MSAPEAPGNGSLIHRQYVFGLLLRLCVTFAGGVGILFVILYFAL
                     SRPLPGDYAGIFHALRNLSPFLRPILAVSFLVYALLTFGSTSALCVYILHKVAGPLYR
                     MELVLDQYRLGAPARTVSFRNGDQIQPLAHAFNQWIGTLRRDRHRWLATMRDAERHCL
                     QDDATCRAKMEEALRKVAEDMARYH"
     gene            complement(1309..1809)
                     /locus_tag="VF357_05340"
     CDS             complement(1309..1809)
                     /locus_tag="VF357_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523603.1"
                     /translation="MKKIFTVVMAVAFVFSVAGFAVAADNASKTGTDPAAVKKGEQAA
                     PMAEKAPMAKKAVKGQKVSGTIEALDAAAGTLTVKGKKGNVDLKAGEEVKLGDFNVGD
                     KVIVKYADGTASSVKGVKAAKAAKKAKKAEKAAPKVDAEPAATPGQPPPAAGKPAGTP
                     TKPAGN"
     gene            complement(1990..3348)
                     /locus_tag="VF357_05345"
     CDS             complement(1990..3348)
                     /locus_tag="VF357_05345"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7523604.1"
                     /translation="MKGTVLVVDDERNQREILGAILKSEGYIPVLASGGAEALRLLER
                     EAVDLVITDLVMPGMTGEELIDAILARNPGMPILLSSAYGTIQTAVDAIKKGAYYYFE
                     KPVDRARLLIIVDRAIENLHLRESHRVLSEKLFPGAVPILGEHAAIREIKRILPRVAR
                     SESTILLTGESGTGKEVIARNVHSLSGRSAAPFLAVNCASLPDTLFESELFGHERGAF
                     TGAMRREIGLFEAAGGGTIFLDEIAEIKLETQAKLLRTLQEKEIRRIGGKENIPVDVR
                     IVAATNRDLDDAVREGKFRADLFYRLNIVKLTLPPLRDRISDIPLLAEHFLAKHGEKG
                     VPPVRDITKEAMRLLMRYPWPGNVRELSSVVERAVVLAEGGKIGPGELPLELREGADA
                     IPARAFDLPPQGVEFEKVEEHLLRQAVGRSGGVHTRGAELLRMSYKAYIYRLKKFGIL
                     PP"
     gene            complement(3345..4805)
                     /locus_tag="VF357_05350"
     CDS             complement(3345..4805)
                     /locus_tag="VF357_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7523605.1"
                     /translation="MKIPGIPLRDRGLQFKLTMVMLILFACSIGSVYIPYIFGRDALK
                     RDLENSFLELSNAISVSVDQLTTPGVSEEDRLYHYVESLKKTGIREVSIVGEDMGVID
                     STNPKAIGRPAKIKLPPEKLVINATFGDDAGEKIQPRDLVVPVKVVGDTLGYIHMRLK
                     IDDFTEPIRRNLYLRLFTTLLIFSGGLVLAVIISAHYVSPVVRLAHAAETVAAGDLSQ
                     ELPVEGNDEVGRLTRSFNEMIVRLRQNRALEEAVRENQYLTHLGKLSSGMAHEIRNPL
                     NFIGLAVDHLDAVTEGKGSEGEAEKRQVIGRIKEEIGRLNDLVTNFILYGRPPELQRA
                     AVRIPELAVGVLLMAEERLRAQSISCRTEFKDTREIHADPDMLRRALVNLVGNAIDAM
                     PNGGTLSVSAGPRPDGKYSIVVEDTGTGIPVNERERIFEPYFTTKVSGLGLGLVLTKK
                     IVDAHGGEIFVDSTQGKGTRIEVALPSGVPVEGAPG"
     gene            complement(4834..5532)
                     /locus_tag="VF357_05355"
     CDS             complement(4834..5532)
                     /locus_tag="VF357_05355"
                     /inference="COORDINATES: protein motif:HMM:NF023238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 family protein"
                     /protein_id="HEX7523606.1"
                     /translation="MRGSRFGKGIALVLVAAVGALSAGCFGKFQLTRKLYDINQSIDE
                     KYVRSAATWLFVIPYAVTGLLDFVVFNVIEFWTGDNPAASAPVTKVYAQGNARTILSL
                     SRDGSATVAVIERYEGERLVSTLRVRDDGLGTVAAVETAAGRKVREVSAVAASDGSVD
                     VTVSTAAGTGTERYAAPSVRAQETRAARIASEVAQASRGPLPPSMAPAALGHPWPSAI
                     SGEAAAARVPAYGG"
BASE COUNT          966 a         2024 c         1781 g         1066 t
ORIGIN      
        1 agacgtactg gcggtggatc aaggggccgt tgcccgccgc ttcggggccg ctcatggggc
       61 gatcctccgg atctgctgct tcagttcgtc ccaccggagg cggaactcct ccggggagat
      121 cccgcccacg gcgagggccg ccgcctcgct ccggatcttc aggacggagg cgagggtgtc
      181 ggagcgcgcg gcgtcgattt tttccccgaa ccggtcgagc tcgccgatgg gacacgtgcc
      241 ggtcggcgtg gaaaggtccc catccgccga cgcccgcagg gaatcgacgg ccttgccgat
      301 gccgtgccgt gtggcggcga taaggagcag gaccaggacc gaaccggcca gaaagacacc
      361 gacgaagaga cccgccaggt gccaggcgag aagatcccgg acgatgtcgt acgcggagcc
      421 gaacgtgtag tgcccccgga tggcggcgtg caggaacacc cgctggaact cgctgtcgac
      481 cagtttccaa aagatcgcgc ccccggttgc gatgatggcc gcgaacgcca gcaccgccgg
      541 tccctggaag cgggggctga cgtagcggat cgtgggccgg cgcggctgca gggcgggatt
      601 tccccggaag agttccgcct ccggtgggac gaactgaagc atctgatccg gaggatcgct
      661 ccatgagcgc ccccgaagcg ccgggcaacg gctccttgat ccaccgccag tacgtcttcg
      721 gccttctgct ccgcctctgc gtcaccttcg cggggggggt ggggatcctt ttcgtcatcc
      781 tctacttcgc gctctcccgg ccgctccccg gggactacgc agggatcttc cacgccctgc
      841 gcaacctctc cccgttcctc cggccgatcc tcgccgtctc cttcctggtt tacgcgctgc
      901 tgaccttcgg ctcgacgtcg gcgctttgcg tctacatcct ccacaaggtg gcggggccgt
      961 tgtaccgaat ggaactggtc ctggaccagt atcgcttggg cgcccccgcg cggacggttt
     1021 ccttccggaa cggcgaccag atccagccgc tcgcgcatgc gttcaaccag tggatcggaa
     1081 cgctccgccg cgatcgccat cggtggctgg ccacgatgag ggacgcggaa cgacactgcc
     1141 tccaggatga cgctacgtgc cgcgcgaaaa tggaagaggc gctccggaag gtcgccgagg
     1201 acatggcgag atatcactga aaaaaaagag gcggggggat tgtgtccccc cgcccgtggg
     1261 tccttggaaa gcccttgtgg ggccggaccg ctacccggaa gaaacggctt aattccccgc
     1321 cggtttcgta ggcgtccccg ccggcttacc cgccgccgga ggcggttgcc ccggcgttgc
     1381 tgcaggctcc gcatcgacct tcggcgccgc cttctccgcc ttcttcgcct tcttcgccgc
     1441 cttcgccgcc ttcacgccct tcacggacga cgcggtgcca tcggcatact tcacgatgac
     1501 cttgtcgccg acattgaagt cgcccaactt gacttcctcg ccggccttca ggtccacgtt
     1561 gcccttcttg cccttcaccg tgagggtgcc ggccgccgcg tcgagcgcct cgatcgtgcc
     1621 agagaccttc tggcccttca ccgccttctt cgccatcggg gccttctccg ccatcggggc
     1681 cgcctgctcc cccttcttca cggccgccgg gtccgtcccg gtcttcgacg cgttgtcggc
     1741 cgcaaccgca aagcccgcga ccgagaacac aaacgcaact gccatcacta ccgtgaaaat
     1801 cttcttcatt tcgttttccc cttccaaagg gagtatttgg atgctttaca taaggatcat
     1861 tcaagaacgg tgccaaccat ttccacaacc ataaccagtc gaaataattc aagttaccga
     1921 tttcgaccca cgcagagctc cctgcctccc tccccattgg aggcaggcat tccccatttg
     1981 gggaatcgat cagggaggaa ggatcccgaa tttcttcaac cggtagatgt acgccttgta
     2041 gctcatccgg agcagctcgg caccccgggt gtgaaccccc ccggaacgcc ccaccgcctg
     2101 ccgcagcagg tgctcctcga ccttctcgaa ctccacgccc tggggaggaa ggtcgaacgc
     2161 cctcgccgga atggcatccg caccctcccg cagctccagc ggaagttccc cgggcccgat
     2221 cttcccgcct tccgcgagga cgaccgcccg ttcgacgacg gaggagagtt cacggacgtt
     2281 cccgggccat gggtaccgca tcaggagccg catcgcctcc ttcgtgatat cgcgcaccgg
     2341 ggggacgccc ttctcgccgt gcttcgccag gaagtgctcc gcaagaaggg ggatgtcgga
     2401 gatccggtcc cgaaggggag gaagagtgag cttcacgatg ttcaaccggt agaagaggtc
     2461 cgcccggaac tttccttccc gcacggcatc gtcgagatcc cggttcgtcg cggcgacgat
     2521 ccggacatcg acggggatgt tctccttccc gccgatccgg cggatctctt tttcctgcaa
     2581 ggtgcgcagc aatttcgcct gggtctccag cttgatctcc gcgatctcgt cgaggaagat
     2641 cgttcccccc cccgcggcct cgaaaaggcc gatctcccgc cgcatcgcac cggtgaacgc
     2701 cccccgctcg tggccgaaca gctcgctctc gaacagggtg tccggaaggg aggcgcagtt
     2761 gaccgcgagg aacggggcgg cgctgcggcc gctcaaggaa tggacgttgc gggcgatcac
     2821 ttccttcccc gtgccgctct cgccggtgag gaggatggtg ctctcgctcc tcgcgacgcg
     2881 cggcaggatc cgtttgatct cgcggatcgc cgcgtgctcg ccgaggatcg ggacggcgcc
     2941 gggaaagagc ttctccgaca gcacccgatg ggactcgcgc aggtgcaggt tctcgatggc
     3001 ccggtcgacg atgatgagca ggcgggcccg gtcgaccggc ttttcgaagt agtagtacgc
     3061 ccccttcttg atcgcgtcga ccgccgtctg gatcgtgccg taggcgctgc tgagaaggat
     3121 gggcatcccg gggttgcggg cgaggatcgc gtcgatcagc tcctcccccg tcatccccgg
     3181 catgacaagg tcggtgatca cgaggtcgac ggcctcccgc tcgaggagcc gcagcgcctc
     3241 cgcacccccg gaggccagca ccgggatgta cccttcactc ttgagaatcg cgccgaggat
     3301 ctcccgctgg tttcgctcgt cgtcgacgac gaggaccgtc cccttcatcc tggcgccccc
     3361 tccacgggga cgcccgacgg aagcgcaacc tcgatccgcg ttcctttccc ctgggtggaa
     3421 tccacgaaga tctccccccc gtgggcgtcg acgatcttct tcgtgaggac cagcccgagc
     3481 cccaacccgg acaccttggt ggtgaagtac ggctcgaaga tcctttcccg ttcattcacc
     3541 gggatgccgg ttcccgtgtc ctccacgacg atggagtatt tcccgtcggg ccggggtccg
     3601 gcggaaacgg acagcgttcc gccgttcggc atcgcgtcga tggcgttccc caccaggttc
     3661 accagcgctc tccgcagcat gtcgggatcg gcgtggatct cacgggtatc cttgaactcc
     3721 gtgcggcagg agatcgattg cgcgcggaga cgctcctccg ccatcagcag cactccgacc
     3781 gcaagctcgg ggatccggac ggccgcacgc tgcagttcgg ggggacgccc gtagagaatg
     3841 aagttggtca ccagatcgtt cagccgcccg atctcctcct tgatccgccc gatcacctgg
     3901 cgtttttccg cttccccctc ggatcctttc ccctccgtca ccgcgtcgag gtggtccacc
     3961 gcgagcccga tgaagttgag cgggttgcgg atttcgtggg ccatccccga ggagagcttc
     4021 ccgagatgcg tcagatactg gttctcccgt accgcctcct cgagcgcccg gttctgccgg
     4081 agccggacga tcatctcgtt gaaggaacgg gtgagccgtc ccacctcgtc attcccctcg
     4141 acgggaagtt cctgcgaaag gtccccggcg gccacggtct cggcggcgtg cgccagccga
     4201 accaccgggg agacgtagtg ggcggagatg atgaccgcga ggacgagccc gccggagaag
     4261 atgaggaggg tggtgaagag gcgcaggtag aggttccgcc ggatcggctc ggtgaagtcg
     4321 tcgatcttca atcgcatgtg gatgtaaccg agggtgtccc ccaccacctt gaccggcacg
     4381 acgaggtccc ggggctggat cttctctccc gcatcatccc cgaatgtcgc attgatcacc
     4441 agtttttcgg gcggcagttt gatcttcgcc ggccggccga tcgccttggg gttggtgctg
     4501 tcgatcacgc ccatgtcctc cccgacgatc gacacttcgc ggatccccgt tttcttcagc
     4561 gattccacgt aatggtacag gcggtcctcc tcggagaccc ccggcgtcgt caactggtcg
     4621 acgctgacgc tgatcgcgtt ggagagttcg aggaagctgt tctcgaggtc cctcttcagc
     4681 gcgtcccgcc cgaaaatgta cgggatgtac accgacccga tggagcaggc gaagaggatc
     4741 agcatgacca tcgtgagctt gaactgcagg ccccgatcac gcaacggaat gcccggaatc
     4801 ttcatgatgc ctccaatatt gctggccggt gcttcagccc ccgtacgccg ggacccgcgc
     4861 ggcggccgcc tctccgctaa tcgccgatgg ccatggatgg ccaagtgccg cgggagccat
     4921 ggatggcggg agcggtcccc gcgacgcctg tgcgacttcc gacgcgatcc gggccgcgcg
     4981 cgtttcctgc gcccggacgg acggcgccgc gtacctttcc gtgcccgttc ccgccgcagt
     5041 ggacaccgtc acgtccaccg acccgtcgga cgccgcgacc gcagaaacct cgcggacctt
     5101 ccttcccgcc gccgtctcga ccgcggcgac cgttcccagc ccgtcgtcgc ggacccggag
     5161 ggtggagacg agcctctccc cttcgtaccg ctcgatcacc gccaccgtcg cggaaccgtc
     5221 acgggagagg ctcaggatcg tcctcgcgtt cccctgcgcg tacaccttcg tcaccggcgc
     5281 ggaggcggcg ggattatcgc ccgtccagaa ctcgatcacg ttgaagacga cgaagtcgag
     5341 gagtccggtc acggcgtacg ggatgacgaa caaccacgtt gccgcgctgc gaacgtactt
     5401 ctcgtcgatc gattggttga tgtcgtacag ttttcgcgtc aactggaact tgccgaagca
     5461 gcctgcggag agcgccccca ccgccgccac cagcaccaac gcgatcccct tcccgaaccg
     5521 gctccctcgc atggcaaccc ccctttcagg ataattacgt attttcgtat tgtaacaggc
     5581 acacaaccca cgttgtagga gtgtccctcg cccacaaccc acaatcccga gggtggtgta
     5641 atattccggg atgacccggg gacaccagcg tgcgtaaaag aacgagcagg gactacctca
     5701 agctcgtcgc gtttctactc ctcgccgccg ccgccgggta cctcctgttc ttcaccccga
     5761 cgggcgccct gttcggaaca cacgagggaa ggaaggcgct ggtcgggaaa ctggacctca
     5821 tcgtccaggc cgcgggg
//
LOCUS       DASVJL010000258         5774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1353001, whole genome shotgun sequence.
ACCESSION   DASVJL010000258 DASVJL010000000
VERSION     DASVJL010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353001"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VF357_05360"
     CDS             complement(<1..214)
                     /locus_tag="VF357_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523607.1"
                     /translation="MATLTKAARMAWQIGAAETARLRHPFIEREHLLIGLCSLRKILQ
                     YLDYTHIESLPVDVLREEADGIERVLA"
     gene            complement(240..1151)
                     /locus_tag="VF357_05365"
     CDS             complement(240..1151)
                     /locus_tag="VF357_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7523608.1"
                     /translation="MSLLVVGSMAFDSIKSPFGEVERVIGGSATYFSLAASYLTPVRL
                     VSVVGKDFPKGTLDMLSARGIDLQGLKVAEGVTFHWKGYYEYDLNIAHTVKTDLNVFE
                     NFAPVLPASYRESRYVFLGNIDPKLQLDILAQVREPKIVALDTMNFWIGKSPQLLREV
                     IRSVDIVFINEAEIRELTGEFNLVKAARKLMRMGPERVVIKRGEYGVLYLSDGEIFAA
                     PAYPLETIFDPTGAGDSFAGGFMGYLASRDGDVLTEMDYRRATIYGSAIASFTVEAFS
                     TERLQGLSLEEIDSRLAAFRALTEFRV"
     gene            complement(1253..2164)
                     /gene="cyoE"
                     /locus_tag="VF357_05370"
     CDS             complement(1253..2164)
                     /gene="cyoE"
                     /locus_tag="VF357_05370"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX7523609.1"
                     /translation="MGERGPRGRDGDIPAPELTPPGNPSAILLAKPGIVAAVTLAGLS
                     GMVLARRGVPPPGKTALTLACILAAAGGSAALNTVLDAGIDEKMPRLTRRIAALRAIG
                     RGNVAAAAVAAIAASLLLSALFLNATTCLLLAAAVAGYAVLYTLVWKRRSPYGTIPGA
                     VPGALPVLIGYAAVNPHIEMDGVLLFLILVLWQPPHFWALALRHQAEYRAAGVPILPV
                     AFGEPYTKVLIFLYAAALLPLSLSLWALGYLSARFGWAAFLLGAGFLAVFYRDTVTSR
                     RFGRAFAVSIVYLTLLLLALLADLLFR"
     gene            complement(2110..2985)
                     /locus_tag="VF357_05375"
     CDS             complement(2110..2985)
                     /locus_tag="VF357_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX7523610.1"
                     /translation="MLGRVTVGLLFLLLVWGNLVAGLKAGLACPDWPLCHGKVLPPFR
                     WDIYMEFGHRVIAAVASIFLVALAARRYKRYGGAARAVPVLALLLLVAEIGMGGAVVL
                     LETPVQLTTVHFMTGVLVFLLAFYMMRFDGERERPTFAFHGPAALFLSVVALVYSQSA
                     LGAYVRHLDAGLACPDFPTCLGKWIPPLLSGSVLAHYSHRTFGYLVLLTAAMLYLFVR
                     RDPRQRGNRSLALTFLVLVAAQIGVGALVVLSGLYYLATALHLAVALGILSVLADLWA
                     NAARAEGTAISLPRS"
     gene            complement(3039..3317)
                     /locus_tag="VF357_05380"
     CDS             complement(3039..3317)
                     /locus_tag="VF357_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX7523611.1"
                     /translation="MTHETTGARRTYFTVYIALVLLTVVTVLISYVNLGLMNVVVALL
                     IASVKASLVALIFMHLKSEDRLVWGFALVPIAFLALIILGTLVDTMLR"
     gene            complement(3325..3915)
                     /locus_tag="VF357_05385"
     CDS             complement(3325..3915)
                     /locus_tag="VF357_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HEX7523612.1"
                     /translation="MSAPPAGHGDPRVAFEAAKLGLWTFLATEVLLFGALFTAYTVFR
                     VKYPALFQSEHLKLNRVLGTLNTLVLITSSLAVALGVDAIRRGKARLLEKYYGVTVLL
                     GAVFLCVKYIEWTEEFHHGLYPGTNIFFSLYFMMTGLHGIHVILGMGVLTYVVVLSRR
                     GRFSGSYHTPVEVAGLYWHFVDLVWIYLLPLLYLIG"
     gene            complement(3912..5516)
                     /gene="ctaD"
                     /locus_tag="VF357_05390"
     CDS             complement(3912..5516)
                     /gene="ctaD"
                     /locus_tag="VF357_05390"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX7523613.1"
                     /translation="MAEESGNGRGYLAERGWRSWALTLDHKRIGGMYLWTTMFFFLVG
                     GVFALLLRLELLSPQARYFSNHTYNVFFTLHGAMMIFLFIIPAIPSGLGNFFIPLHIG
                     ARDVAFPRLNLASYWVFVAGFLVIIGSLVAPIDTGWTFYTPYSAKSTAAITTLSLGLF
                     LIGMSSIFTGLNFIVTIHKLRAPGMTWHRLPLFIWGMYATSIIQVIATPVVGVTFLLL
                     AAERLFGVGFYDPSKGGDPVLFQHFFWFYSHPVVYVMILPAMGIISEVIPVFSRKPIF
                     GYKAIAYSSLAIAFLGFLVWGHHMFTSGMSPLANAIFSFFTFFVAVPTAVKVFNWIAT
                     LYKGSITFESPMLYALTFIFLFAVGGLTGPFLGALATNVQLHDTYFVVAHFHYTMMGG
                     TVMGFFAGLHYWFPKMTGKMLNEKVARIAWALVFVGFNVAFFTMFIVGVRGMPRRYAE
                     YLPKFHVENVISTIGAAILAVGIAVMFWNFFQGLRKGPPAPGNPWRALSLEWQIPSPP
                     DTENFHEIPVVTDWPYGYGKPKGTVA"
     gene            complement(5539..>5774)
                     /locus_tag="VF357_05395"
     CDS             complement(5539..>5774)
                     /locus_tag="VF357_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523614.1"
                     /translation="AVDGKQKMAPGFRGLYGSRVPLEGGISVTADEEYLRESIVDPGA
                     KVVKGYPNVMPTFKATLSKEDIDAVVAYLKTLK"
BASE COUNT         1180 a         1851 c         1904 g          839 t
ORIGIN      
        1 gcgcgagaac ccgttcgatc ccgtccgcct cctcccgaag gacatcgacc gggagcgact
       61 cgatgtgggt gtagtccagg tattgcagga ttttccggag gctgcacagg ccgatcagga
      121 ggtgctcccg ctcgatgaac ggatgccgaa gccgggccgt ctcggccgcg ccgatctgcc
      181 aggccattct ggcggccttg gtcaacgtcg ccatggtcga agtccctccc cccccacgtt
      241 caaacccgga attcggtgag ggcccggaac gccgcgaggc gggagtcgat ctcttcaaga
      301 gagagcccct gcagccgctc cgtgctgaac gcctccaccg tgaaggaggc gatggcgctg
      361 ccgtagatcg tggcccgccg gtaatccatc tccgtgagca cgtctccgtc ccgcgacgcg
      421 agatatccca tgaagccccc cgcgaagctg tcccccgccc cggtggggtc gaagatcgtc
      481 tccagagggt acgccggggc cgcgaagatc tccccgtcgg acaggtagag gacaccgtat
      541 tccccccgct tgatcacgac gcgctccggc cccatgcgca tcagcttccg ggccgccttg
      601 acgaggttga actcgcccgt cagctcccgg atctccgcct cgttgatgaa gacgatgtcg
      661 acgctccgga tcacctcccg cagcagctgc ggactctttc cgatccagaa gttcatggtg
      721 tcgagcgcga cgatcttcgg ttcgcgcacc tgggccagaa tgtccagctg cagtttcggg
      781 tcgatgttcc cgaggaagac gtagcgggat tcccggtacg acgccggcag gacgggagcg
      841 aaattctcga acacgttcaa gtccgttttt accgtgtggg cgatgttcag gtcgtactcg
      901 taatacccct tccagtggaa ggtgaccccc tcggcgacct tcaacccctg gaggtcgatc
      961 ccccgggcgg agagcatgtc gagcgtcccc ttcgggaagt cctttcccac gaccgagacg
     1021 agacgcacgg gcgtcaggta gctcgccgcg agcgagaagt aggtcgccga cccgccgatc
     1081 acccgctcca cctcgccgaa cggcgacttg atgctgtcga acgccatcga tcccacgacc
     1141 agaaggctca ttctccgctc ccggggattc aagaagatag aacatcacct ccatgatacc
     1201 gcaggagggg ggaaaagaag gaccggaaga tccggtcggg ttccccgagg ggtcaccgga
     1261 acaggaggtc ggcgaggagg gcgaggagca gcagcgtcag gtagacgatc gagacggcga
     1321 acgcgcggcc gaagcgacgg ctcgtcacgg tgtccctgta gaagacggcg agaaaccccg
     1381 ccccaagcag gaaggccgcc catccgaaac gggcggaaag gtaccccagc gcccatagcg
     1441 acagggacag gggaagcagt gccgccgcgt acaggaagat cagcaccttg gtgtacggct
     1501 cgccgaacgc gacggggagg atcgggacgc ccgccgcgcg gtactccgcc tggtggcgca
     1561 gcgccagggc ccagaagtgg ggcggctgcc agaggacgag gatcaggaac aggagaacgc
     1621 cgtccatttc gatatgcgga ttcacggcgg cgtacccgat gaggaccggc agggccccgg
     1681 gaacggcgcc ggggatcgtc ccgtacggcg agcggcgctt ccacaccagt gtgtacagga
     1741 ccgcgtatcc agccacggcg gcggcaagga gaaggcacgt cgtggcgttg aggaagaggg
     1801 ccgaaaggag gagcgaggcg gcgatcgcgg ccaccgccgc cgccgcgacg ttcccgcgcc
     1861 cgatggcccg caaggcggcg atccggcggg tcaggcgcgg catcttctcg tcgatcccgg
     1921 cgtcgaggac cgtgttcagg gcggcggacc cgccggccgc cgcaaggatg caggccaacg
     1981 tgagcgccgt ctttccggga ggagggacac cccgccgggc gagcaccatc ccggagaggc
     2041 cggccagggt caccgccgcg acgatccccg gcttggccag caggatcgcg gacgggttcc
     2101 ccggcggtgt cagctccggg gcagggatat cgccgtccct tccgcgcggg ccgcgttcgc
     2161 ccaaaggtcg gccaggaccg acaggatccc cagcgccacc gcgaggtgca gcgccgtggc
     2221 gaggtagtag agccccgaca gcaccaccag ggcccccacg ccgatctggg ccgcgacgag
     2281 gaccaggaac gtcagggcga gggatcgatt cccccgctgg cgcgggtcgc gccggacgaa
     2341 gaggtagagc atcgccgcgg tcagcagcac gaggtacccg aacgtccggt gggagtagtg
     2401 cgccagcacg gacccggaga ggagcggcgg gatccacttc ccgaggcagg ttgggaagtc
     2461 cgggcaggcc agccccgcgt caaggtggcg gacatacgcc ccgagggcgg attgcgaata
     2521 gaccagcgcc acgacggaga gaaagagggc cgccggtccg tggaaggcga aggtgggtcg
     2581 ttcacgctct ccgtcgaatc tcatcatgta gaaggcgagt agaaacacca gcaccccggt
     2641 catgaagtgg accgtggtca gctgcacggg ggtttcgagg agcaccacgg ccccgcccat
     2701 cccgatctcc gccacgagca ggaggagggc gagaaccgga accgcccgcg ccgcgccgcc
     2761 gtacctcttg tagcgtcgcg ccgcaagggc gaccaggaag atcgacgcca cggccgcgat
     2821 gacgcgatgg ccgaattcca tgtaaatgtc ccagcggaaa gggggcagga ccttcccgtg
     2881 gcacaacggc cagtcggggc acgccagccc cgccttgagt ccggcgacga ggtttcccca
     2941 gaccagcaga aggaacagca gccccacggt cactctgccc agcatcggtg tccctcctcc
     3001 ggaatgcgga tacggggaat tatccccacc gcgggggatc agcgcaacat cgtgtcgaca
     3061 agcgtcccga gaatgatgag cgcaaggaaa gcgatcggca ccagggcgaa tccccacacc
     3121 aggcggtctt cgctcttgag gtgcatgaag atcagggcca ccagggacgc cttcacggag
     3181 gcgatcagca gcgccacgac cacgttcatc agcccaagat tgacgtagga gatcaggacg
     3241 gtgaccaccg tcaacaggac cagggcgatg tagaccgtaa aataggtccg gcgggcgccg
     3301 gtcgtttcgt gggtcatgac tccctcaccc gatcaggtaa agcagcggca acaggtagat
     3361 ccacaccagg tcgacgaagt gccagtacag gcccgccacc tccaccggtg tgtggtaact
     3421 cccggagaat cgtccccgcc tcgagaggac aacgacgtac gtgaggaccc ccatgccgag
     3481 aatcacgtgg atgccgtgca gccctgtcat catgaagtag agcgagaaga agatgttcgt
     3541 acccggatac aggccgtggt gaaactcctc ggtccactcg atgtacttca cgcagaggaa
     3601 gaccgctccc aggagaaccg tcaccccgta atacttctcc agaagacgcg ccttccctct
     3661 gcggatcgcg tccacgccga gcgccaccgc caggctgctg gtgatcagga ccagggtgtt
     3721 cagggtcccc agaacgcggt tgagcttcag gtgctccgac tggaacaacg ccgggtactt
     3781 cacgcggaag acggtgtacg cggtgaaaag agcgccgaag aggagcacct cggtcgcgag
     3841 aaaggtccac aggccgagct tggcggcctc gaatgcgacg cgcgggtcgc cgtgcccggc
     3901 gggaggagcg ctcacgcgac cgtccctttc ggtttcccgt aaccgtacgg ccagtcggtc
     3961 acgacgggga tctcgtggaa gttctcggtg tcgggcggcg aggggatctg ccactccagg
     4021 gacagagcgc gccagggatt ccccggcgca ggcgggcctt tccgcaaacc ctggaaaaag
     4081 ttccagaaca tcaccgcgat ccccacggcg aggatcgccg cgccgatggt cgagatcacg
     4141 ttctcaacgt ggaacttggg gaggtactcc gcgtaacggc gcggcatgcc tcttaccccg
     4201 acgatgaaca tggtgaagaa ggcgacgttg aagccgacga aaaccagcgc ccatgcgatc
     4261 cgcgcgacct tctcattcag catcttcccc gtcatcttcg ggaaccagta gtggagcccc
     4321 gcgaagaacc ccatcaccgt gccgcccatc atcgtgtagt ggaagtgggc cacaacgaag
     4381 taggtgtcgt ggagctgcac gttggtggcc aacgcgccga ggaacggccc ggtgagtccc
     4441 ccgacggcga acaggaagat gaaggtcagg gcgtacagca tcggcgactc gaaggtgatg
     4501 gaccccttgt agagcgtggc gatccagttg aacaccttca ccgccgtcgg gacggcgacg
     4561 aagaaggtga agaacgagaa gatcgcgttc gccagcgggg acatgcccga ggtgaacatg
     4621 tggtgccccc ataccaggaa cccgaggaac gcgatcgcca gcgaggaata ggcgatcgcc
     4681 ttgtacccga agatcggctt gcgggagaag accgggatca cctcggagat gatccccatc
     4741 gccggcagga tcatcacgta gaccacgggg tgggagtaga accagaagaa gtgctggaag
     4801 aggaccgggt cgcctccctt ggacgggtcg tagaacccca ccccgaacag gcgctccgcg
     4861 gcgagcagga ggaacgtcac cccgaccacc ggcgtggcga tcacctggat gatgctcgtg
     4921 gcgtacatcc cccagatgaa gagggggagc cggtgccatg tcatccccgg ggcgcgcagc
     4981 ttgtggatcg tgacgatgaa gttgagtccg gtgaagatcg acgacatccc gatgaggaag
     5041 agcccgaggg agagggtcgt tatcgcggcg gtggatttcg ccgagtaagg cgtgtagaag
     5101 gtccaacccg tgtcgatcgg cgccacaagc gacccgatga tcacaaggaa gccggcgacg
     5161 aagacccagt agctcgccag attcagccgc gggaaggcga cgtcgcgggc ccctatgtgg
     5221 agagggatga agaagttccc cagccccgag ggaatcgccg ggatgatgaa gaggaagatc
     5281 atcatcgccc cgtgaagggt gaagaagacg ttgtaggtgt ggttgctgaa atatcttgcc
     5341 tggggggaga gcagctccag ccgcagcagc agggcgaaca cgccgccgac caggaagaag
     5401 aacatcgtcg tccagagata catccccccg atccgcttgt ggtcgagggt gagcgcccag
     5461 gaccgccacc cgcgctccgc caagtacccc ctgccgttcc cggactcctc cgccatcggt
     5521 accacccctc tcaccgcctc acttgagcgt cttcaggtac gccaccaccg cgtcgatgtc
     5581 ctctttcgac agcgtggcct tgaacgttgg catcacgttt gggtacccct ttaccacctt
     5641 tgcgcccggg tccacgatcg actcccgcag atattcctcg tcggccgtaa cgcttattcc
     5701 tccctcgaga gggaccctcg agccgtacag cccgcggaag ccgggggcca tcttctgctt
     5761 cccgtcgacg gcgt
//
LOCUS       DASVJL010000259         6192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_978155, whole genome shotgun sequence.
ACCESSION   DASVJL010000259 DASVJL010000000
VERSION     DASVJL010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978155"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VF357_05400"
     CDS             complement(<1..612)
                     /locus_tag="VF357_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7523615.1"
                     /translation="MKWGWIAALVLCILALAGYVFLARSGKEQSGADKKGGGAAARSV
                     PVTAVPAKKGDIGVYLAGLGSVAPNNTVTVRSRVDGQLMEVLFREGQIVRSGELLARI
                     DPRPFEAQLAQVEGQLARDQALLKNAELDLARYRTLLQQDSIASQQIDTQEALVRQYE
                     GTVKADQGQVESARLQLTYSRITAPIGGRVGLRLVDAGNMIHAT"
     gene            886..1251
                     /locus_tag="VF357_05405"
     CDS             886..1251
                     /locus_tag="VF357_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523616.1"
                     /translation="MLEEFRRWIHRHLNVVLSFVIGLTFGISILALAYFSTRNDVAAE
                     ALRYGVQITGHSDIGEDIVWFFCGILVGASAICLAWWRDSEAQSVGLENADVLNRLIT
                     DPTPPIVRRPERSRQDADV"
     gene            1279..2124
                     /gene="nudC"
                     /locus_tag="VF357_05410"
     CDS             1279..2124
                     /gene="nudC"
                     /locus_tag="VF357_05410"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX7523617.1"
                     /translation="MLFLPGVVPPPTLSDNAWWFLFHGDRLLVEARDVEVAIPRWASP
                     EEAGLLPVRTQFLGTLDGTPCYSGELAGEAGPKGTSFRALRPLLGVLPEEMFSLAGRA
                     FQVMDWDRTHQFCGKCGGRTEPLEGERGRACAACGLHFFPRVTPAIIVAVVRNGKILL
                     AQSSRFPTAFYSVLAGFVEPGETFEECVRREIREEVGIEVENLRYFGSQPWPFPHSLM
                     VGFTALYAGGDLVLEEKEIVQAGWFGAEEVLRLRIPHHGTIARRLIDRFLAEPPPQIS
                     SYDRS"
     gene            2151..2852
                     /locus_tag="VF357_05415"
     CDS             2151..2852
                     /locus_tag="VF357_05415"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX7523618.1"
                     /translation="MTRIYRVIPAILVLFLAIPSATSIAASEEAGRIESATEVLSKIM
                     EIPESSIPPALLADAQGIAIIPGVLKVGFVVGGQYGRGVLVVRGKGGAWSNPVFISLM
                     SGSIGWQIGAESTDFVLVFKTPRSIEGIMKGKYTLGADAGVAAGPVGRVAKAATDIEL
                     KAEIYSYSRNRGLFAGISLEGSSLQVDDKSNAAYYEKEEVRPSGILSGKEVKTPAGAE
                     KLKNTLEKHAPAAAN"
     gene            3032..3364
                     /locus_tag="VF357_05420"
     CDS             3032..3364
                     /locus_tag="VF357_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7523619.1"
                     /translation="MKMSGKIVVLVVFVGLVLPAAGLYAAEKKESDGAKLFQQNCAAC
                     HPNGGNSVKPALTLSKKDRDANGVKATNDIVGKMRNPGPGMTRFDAKTISDKDAKEIA
                     EYILKTFK"
     gene            complement(3396..>6192)
                     /locus_tag="VF357_05425"
     CDS             complement(3396..>6192)
                     /locus_tag="VF357_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HEX7523620.1"
                     /translation="SGLRFGGTVEIDGTRAVVSVARMSLESPRLRTSGRLVLDRAVPR
                     AALEMHGGDLDIAPVRGALLSLAGDVAGVRDVLDYVRGGRLTLSALRMEGPTIFGRGW
                     IDRLFLEGRLGAGNIFVPDADLDLRDVSGNVVLSGGILTADHSEARSGDSRAREGSLR
                     MGVTGRNPPFRLDAQVEADLARLPRVLLRLIKDKGLEDELSRIENLQGKASGRLTIGD
                     RIGSLRVAVDATDLRLSARYRRVPFPVEVDGGRLRFDENGIAVERLSGRLGRSAFSGV
                     AAHVRLGDSPAFERLSGNASIALGELYPWLASLDGMKTARENVRRLDGFTDLVIARLD
                     GPIARPAEWRYEAAGSLRDVVLEATRLPEPIAMAKGDFRVSPATLSVTALDARFLDAA
                     VLLSGQVDGYREPFRKLDATLSGQVGPDALRWIWAAARMPPELIPHAPVSVSAGHLVL
                     DMSGTFSIAGTYTLPKGLVLSGEFIKPRDGFVLQHLVVRDDESLATIDLRLKEKELDV
                     GFSGHLARATVSRLFQNAKIRHGWITGDLRAHIPLNLPARSTAQGQLVAKDLHIPRLM
                     GPLAIEDLSLRATGNRIAVTSSALAWGKTRFSLTGEAIASGDALTVDVDLASDGIAWD
                     ELRATLPGAGPSQGAAATGGTGIARGGSGPWPLPVTGVIRLSAGSLTVGEYAWKPFRA
                     DIVLRKEEITTTIREADLCGIATTGDVHLGPGGAEVDLRLSTRRHEIEFALSCLRHQQ
                     IGMTGTYELTGHIAGRGTGDALVRALHGDIEFRAVKGRIYKLNLLSKILALLNVTQFF
                     LGKVPDLAHDGFAYNALAVKGTLSDGKLKIGEAVLDGSSMNIVGQGEVDVRTRKVDIV
                     ALASPLKTIDTILRMIPVVRYIMGGSLVSVPVKAEGDLEDPTVSILAPSAVAKGLLGV
                     VERVLKLPVRIFEGGVP"
BASE COUNT         1129 a         1983 c         1977 g         1103 t
ORIGIN      
        1 ggtcgcgtgg atcatgttcc ccgcgtccac gagccgcagc ccgacccggc cgccgatcgg
       61 agcggtgatg cgggaataag tcagctgaag cctggcgctt tcgacctgcc cctggtccgc
      121 cttgacggtg ccttcatatt gccggaccag cgcttcctgc gtgtcgatct gctggcttgc
      181 gatcgaatcc tgctggagca gggtgcggta gcgcgcaagg tcgagttcgg cgtttttcaa
      241 aagcgcctgg tcgcgcgcca gctgcccctc gacctgggcg agctgcgcct cgaacggccg
      301 gggatcgatg cgggccagaa gttccccgct ccggacgatc tgcccttcgc ggaacaggac
      361 ctccatcagc tgcccgtcca cgcgactcct gacggtgacc gtgttgttcg gcgcgacgct
      421 tccgaggccg gcaagataga ccccgatgtc gccctttttc gccgggacgg ccgtgaccgg
      481 cacgctgcgc gccgcggcgc cccctccttt tttgtctgca ccggattgct cctttcccga
      541 tcttgccagg aagacgtacc cggccagcgc caggatgcag aggacaagcg cggcaatcca
      601 tccccatttc aagcgtttcc cgggtatcgc ccgctcgtct ccagcttccg tgtgagccga
      661 agacgccacg gtattctgcg gaatgtcgtc ttttcgggaa tcattctcag ggtctcggct
      721 tttggacata tctcccccgc ccggtgattt atatattcgg cgaatctcgt ccccgaaagg
      781 tttcgaggaa tataatactt gggaagaaag ggggaaggat cgtaaccttc aggccgtatc
      841 gtacgccttc tcttgaaaac aggggaaatg attgggggtg ccgtgatgct ggaggaattt
      901 cgccgttgga tccatcgcca cctcaacgtc gtcctgtcct ttgtgatcgg tttaaccttt
      961 ggaatctcca tccttgcgct tgcgtatttc tccaccagga acgatgttgc cgccgaagcg
     1021 cttcgatacg gtgttcagat caccggccac tcggacatcg gcgaagacat cgtctggttt
     1081 ttctgcggga tccttgtcgg cgcctcggcc atctgcctgg cttggtggcg ggacagtgaa
     1141 gcgcaatcgg tcggtcttga gaatgcggat gttttgaacc gtctaatcac ggatccaacg
     1201 cccccgatcg ttcggaggcc ggaaagatcc cggcaggatg ccgatgtgta gtcggattcc
     1261 ctctctctaa ggattccgat gctctttctc cccggcgtcg tgccgccgcc aaccctttcg
     1321 gacaacgcgt ggtggttcct gttccacggt gaccggctcc tggtggaagc ccgtgacgtc
     1381 gaggtcgcga ttccgcgctg ggcatccccg gaagaggcgg ggctattgcc cgtccgaacg
     1441 caattcctcg gaacgctgga cggcactccc tgctactccg gtgaactggc cggggaggcc
     1501 ggaccgaaag gcacgtcctt ccgcgcgcta cgccctttgc tcggtgtgct cccggaagag
     1561 atgttttcgc tcgccggaag ggcgtttcag gtcatggact gggatcggac gcaccagttt
     1621 tgcgggaagt gcggaggacg taccgaaccg ttggaagggg agcgggggag ggcctgcgcc
     1681 gcgtgcgggc tccatttctt cccgcgggtc acgccggcca tcatcgtcgc cgtggtccgg
     1741 aacggcaaga tcctccttgc gcaatccagc aggttcccca cggccttcta cagcgtgctg
     1801 gccggattcg tcgaaccggg agagacgttc gaggagtgcg tgcgccggga aatccgggaa
     1861 gaggtcggga tcgaggtaga gaacctgcgg tacttcggaa gccagccgtg gccgttcccg
     1921 cactccctga tggtcggctt caccgctttg tacgccggcg gagatctcgt tctcgaggaa
     1981 aaggagatcg ttcaggcggg ctggttcggc gcagaggagg tcctccggct ccggataccg
     2041 catcatggca ccatcgcccg gcggttgatc gatcgattcc tcgcggagcc ccccccgcag
     2101 atctcatcgt atgatcgttc gtgatccgac gtgaaaggag aagaacagcc atgacgagaa
     2161 tttaccgggt tattccagcg atccttgtcc tgttccttgc gatcccttcg gcgacctcga
     2221 ttgcggcgag cgaggaggcg ggacggattg agtccgccac cgaggtattg tccaagatca
     2281 tggagattcc cgagagctcg ataccccccg ccctgctggc ggacgcccag gggatcgcca
     2341 tcatccccgg cgtcctcaag gtcgggttcg tggtgggagg acagtacggc cgcggtgtcc
     2401 tcgtcgtccg cggaaagggc ggcgcatgga gcaatccggt gttcatttcc ctgatgagcg
     2461 gaagcatcgg ttggcagatc ggcgccgaat ccactgactt cgtccttgtt ttcaagaccc
     2521 cccggagcat cgagggcatc atgaagggaa aatacacctt gggggcggac gcgggggtgg
     2581 cggcgggtcc ggtgggacgg gtggcgaagg cggccaccga catcgagctg aaggcggaga
     2641 tctattccta ctcccggaac cgtggactct tcgccggcat ctccttggag ggatcctccc
     2701 tgcaggtgga cgacaagagc aatgccgcgt attacgaaaa ggaagaggtc cgtccttcgg
     2761 ggatcctctc ggggaaggag gtcaaaacgc ccgcgggtgc ggaaaagttg aaaaatacgc
     2821 tggaaaagca tgctccggcg gccgcgaatt aaaggccggg agcgccccgc ggagacaagg
     2881 gtacatcaca tggaagtacc ctgtcttctt tgatcatcgg accggaaaat tcgtcgtctc
     2941 ttatcatcgt tccgagatga taggataggg ataagtcgga aggaaactcc gggaacgaac
     3001 gcaacgattc gacgagacaa aggaggggaa gatgaaaatg tcgggaaaga tcgtcgttct
     3061 tgtggtcttc gtgggcctgg tccttcccgc cgctggcctc tacgcggcgg agaagaagga
     3121 atcggacggg gcgaagctgt ttcagcagaa ctgcgccgcc tgccacccga acggggggaa
     3181 cagcgtcaaa ccggccctca cactcagcaa gaaggacagg gatgccaacg gtgtcaaggc
     3241 gaccaacgac atcgtaggga agatgcgaaa cccgggaccc gggatgaccc gcttcgacgc
     3301 gaaaacgatt tccgacaagg atgcgaagga gatcgcggag tacatcctca agactttcaa
     3361 ataatcgggg aggctggaac agggtcgtcg ccccgtcatg ggacaccacc ttcgaagatc
     3421 cgtacgggga gcttgagcac ccgctccacc accccgagga ggccctttgc gaccgccgac
     3481 ggcgctaaaa tggagacggt cggatcctcc agatcgccct ccgccttgac cgggacggag
     3541 acgagcgagc ctcccataat gtatctcacg acggggatca tccggaggat cgtgtcgatg
     3601 gtcttcaacg gggaggccag cgccacgatg tccaccttcc tcgttcggac gtcgacctct
     3661 ccttgtccaa cgatgttcat cgatgagccg tccagcaccg cttccccgat cttcagcttt
     3721 ccatccgaga gggttccctt gacggccaac gcgttgtacg cgaatccgtc atgcgccagg
     3781 tccgggacct tcccgaggaa aaactgggtc acattcagca gggcgaggat cttcgacagg
     3841 agattgagct tgtagatccg tcccttgacg gcgcggaact cgatatcccc atggagcgcg
     3901 cggacgagcg cgtcgcctgt cccgcgcccc gcgatgtggc cggtgagttc gtacgtcccc
     3961 gtcatgccga tctgttgatg ccgcagacac gacaaggcga actcgatctc atgccgccgc
     4021 gttgacaacc ggagatcgac ctccgccccg ccgggaccga gatgcacgtc gcccgtggtt
     4081 gcgatgccgc agagatcggc ctcccggatc gtcgtcgtga tctcttcctt ccgaaggacg
     4141 atatctgcgc ggaacggctt ccatgcgtat tccccgacgg tcagggaacc ggcggacagc
     4201 cggatgactc cggtgaccgg tagcggccag ggtccggatc cgccgcgggc gatgccggtt
     4261 cctcccgtcg ccgcggcccc ttgcgatggt cccgcgccgg gaagcgtcgc gcggagctcg
     4321 tcccacgcga ttccgtcgga agcgagatcc acgtccacgg tcagcgcgtc cccggaggcg
     4381 atcgcctcgc ctgtcagcga gaagcgcgtc ttcccccagg cgagcgcgga cgacgtcacg
     4441 gcgatccggt tccccgtcgc gcgcagcgac agatcctcga tggccagcgg ccccatcagg
     4501 cgcgggatat gtagatcctt cgcgaccagt tgcccctgcg cggtggagcg ggcggggagg
     4561 ttcagcggga tgtgggcccg aagatcccct gtgatccagc cgtgccggat cttcgcgttc
     4621 tgaaacaggc ggctgacggt cgccctcgcg aggtgacccg agaatcccac gtccaactcc
     4681 ttctccttca gccgcagatc gatcgtggcg agagactcgt cgtcccgcac gaccagatgt
     4741 tgaaggacga atccgtcgcg aggcttgatg aattcccctg aaagcacgag gcccttcgga
     4801 agggtgtacg tgcccgcgat cgagaacgtt ccgctcatgt caaggaccag gtgacccgcc
     4861 gagacggaga ccggggcatg ggggatcagc tccggcggca tgcgggccgc ggcccagatc
     4921 caccggagcg catccggccc aacctggccg gagagcgtcg cgtcaagctt ccggaagggc
     4981 tcccggtagc cgtcgacctg ccccgagagc aggaccgcgg cgtccaggaa cctcgcatcc
     5041 agtgccgtga cggagagggt cgccggcgaa acacgaaaat cgcccttcgc catcgcgatg
     5101 ggttccggca ggcgcgtcgc ctccaggacg acatcccgca gcgacccggc ggcctcgtat
     5161 cgccactcgg cgggccgggc gatcggtccg tccagccggg cgatgacgag atccgtgaat
     5221 ccatcgagcc tccggacgtt ctcccgtgcc gtcttcatgc cgtccaggga cgccagccac
     5281 ggatagagct ctccgagcgc gatggacgcg tttcctgaga gccgctcgaa ggcgggcgaa
     5341 tcgccgagac ggacgtgcgc ggcaaccccc gagaacgcgg agcgtcccag ccggccggac
     5401 agacgctcca cggcgatccc gttttcgtcg aagcgaagcc ggccgccgtc gacctcgacc
     5461 gggaacggga ctctccggta ccgggcggac aggcgaagat cggtggcgtc gaccgcgacc
     5521 cgaagcgacc cgatccggtc gccgatcgtc aaccggccgg acgccttccc ctggagattc
     5581 tcgatgcgcg acagctcgtc ttcgagcccc ttgtccttga tcaagcgcaa caggacgcgc
     5641 gggagccgcg cgagatccgc ttccacctgc gcgtccagcc ggaacggggg attgcgcccg
     5701 gtgactccca tcctgagcga tccttcgcgt gcccgcgaat cgccggatct cgcctcggag
     5761 tgatcggccg tcaggatccc ccccgaaagg acgacgttcc ccgagacatc ccggaggtcg
     5821 agatccgcat cggggacgaa gatgttcccc gcaccgaggc gcccctcgag gaagagacgg
     5881 tcgatccatc cgcgaccgaa gatcgtcggt ccctccattc tcagcgccga cagcgtcaga
     5941 cgacctcccc ggacgtagtc cagcacatcg cgcacgccgg cgacgtcgcc ggcgagagag
     6001 aggagcgctc cccggaccgg cgcgatgtcg aggtcgcccc cgtgcatctc cagcgcggct
     6061 cgagggacgg cgcgatcgag caccagccgt ccggaggtcc ggagccgggg cgattcgagc
     6121 gacatccgcg ccaccgagac gaccgctctc gtaccgtcga tctcgaccgt tccgccgaac
     6181 cggagaccgg ac
//
LOCUS       DASVJL010000260         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_510087, whole genome shotgun sequence.
ACCESSION   DASVJL010000260 DASVJL010000000
VERSION     DASVJL010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510087"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VF357_05430"
     CDS             complement(<1..456)
                     /locus_tag="VF357_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX7523621.1"
                     /translation="MRKLIAGIHRFQKQHWSKNQELYRRLAEHGQFPEALFITCSDAR
                     VDPVTITHGQPGDLFIVRNMGNFVPPYSENPLEGTGVAAAVEYAVEHLEVRDIVVCGH
                     SDCGAMKALYREREMFAATPHIGQWLRHGDRTMAVVAANYPDRSREERYE"
     gene            667..1371
                     /locus_tag="VF357_05435"
     CDS             667..1371
                     /locus_tag="VF357_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7523622.1"
                     /translation="MTKKTAFWIFLIGTISSAAVFLWATYDTHRQVATLSHVDKLSDQ
                     VVAGKRAFEKYNCNDCHTILGFGGYYAPDLTKVVQRVGEEGIRYRVKSPEMAFANSPR
                     KMPNQHVTDQEITDLVAFFRWVGEINNNDWPPQDSKKRLSRGEQMMVAKVGVSPGAAV
                     FQTKGCMNCHSLKGTGGTFGPALDKIGAGMSAEEIGKYVRNPKGVNPGSKMPSQKDNL
                     SERELDEVARFLATLK"
     gene            1382..>2359
                     /locus_tag="VF357_05440"
     CDS             1382..>2359
                     /locus_tag="VF357_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX7523623.1"
                     /translation="MEYRSQKIAYWYFAAALPLFVLQVVVGLWLAANYTFTIPQSIVN
                     VFPFSTARAFHTNLLVLWMLLGFMGGTYYIIPEETKSEIYSEKLAWFQLIALLVTGVT
                     ALVGFLFGWTQGRPLLEIPMALDFVVVICALVFLFNVGMTMFKAKNWTAIQGTLLGGL
                     VFLALLYLFGIPFYRNLVLDWYYWWWVIHLWVEGAWELVTASIFAFVLMKITGVEREV
                     VEKWLYVEIGLFLFTGLAGTGHHYYWIGAPRYWLWIGGVFSALEPLPILLMVIDTMHH
                     VKERKTKIINPLTWTYAIGCAIMHLIGAGVWGFLHTLPQINYYTHGSQVT"
BASE COUNT          422 a          759 c          728 g          450 t
ORIGIN      
        1 ctcgtaccgt tcctcccggg accggtccgg gtagttggcc gccaccactg ccatcgtccg
       61 atccccatgg cgcagccact ggccgatgtg gggggttgcg gcgaacattt cccgctcccg
      121 gtagagcgcc ttcatcgcgc cgcagtcgga gtggccgcag acgacgatgt cccgcacctc
      181 caagtgttcc acggcgtact ccaccgccgc cgccacgccg gtgccctcca ggggattctc
      241 cgagtacggc gggacgaagt tccccatgtt cctgacgatg aagaggtccc ccggctgccc
      301 gtgggtgatc gttacggggt cgacccgggc gtcggagcag gtgatgaaca gcgcctcggg
      361 gaactgcccg tgctccgcca gccgccggta cagctcctgg ttcttgctcc aatgctgctt
      421 ctggaaccga tggatccccg cgatcagctt tcgcatgacg aaggtccccc cagcggcgat
      481 ttctcctgat gatacacggt attcccagag tctttccgcg atttttcccg tcttgacctg
      541 gatcaatgaa ggataaatga tacccgggta gcattctccc caatcgattt gtattcgcgg
      601 cgtggcggga gcaaccaaac cagatgccgg cggcaacttc gccgccggga caaatggagg
      661 ggacggatga cgaagaagac cgccttctgg attttcctga tcggcacaat ctcctctgcg
      721 gccgtgttcc tgtgggccac ctacgacacc caccggcagg tggcgacctt gtcgcacgtg
      781 gacaagctgt cggaccaggt cgtggccggg aaacgggcgt tcgagaagta caactgcaac
      841 gactgccaca cgatcctggg cttcggcggc tactacgcac cggacctgac gaaggtggtc
      901 cagcgggtcg gcgaggaggg gatccgctac cgggtgaagt cgcccgagat ggcgttcgcc
      961 aactccccgc ggaagatgcc gaaccagcac gtcacggacc aggagatcac cgatctcgtc
     1021 gccttcttcc ggtgggtcgg cgagatcaac aacaacgact ggccgccgca ggacagtaaa
     1081 aaacggctgt ctcggggcga gcagatgatg gtcgcgaagg tgggggtgtc gccgggagcc
     1141 gccgtcttcc agacgaaggg gtgcatgaac tgccactccc tcaaggggac gggagggacg
     1201 ttcgggcccg cgctggacaa gatcggggcg gggatgtcgg cggaggagat cgggaagtac
     1261 gtccggaatc ccaagggcgt caacccgggc tccaaaatgc cgtcgcagaa ggacaacctc
     1321 tccgagcggg aactggacga agtcgccagg ttcctcgcga ccctgaaata aggaggcccc
     1381 gatggaatat cgctcccaga agatcgccta ctggtacttc gccgccgcgc tcccgctctt
     1441 cgtgctccag gtcgtcgtgg ggctgtggct cgccgccaac tacactttca ccatcccgca
     1501 gtcgatcgtg aacgtcttcc cgttctcgac ggcgcgcgcg ttccacacga acctcctcgt
     1561 cctgtggatg ctcctgggct tcatgggcgg cacctactac atcatccccg aggaaacgaa
     1621 gtcggagatc tactccgaga aactcgcctg gttccaactg atcgccctgc tcgtcacggg
     1681 cgtgacggcc ctcgtggggt tcctcttcgg gtggacgcag gggcggccgc tcctggagat
     1741 cccgatggcg ctcgacttcg tcgtcgtcat ctgcgctctc gtcttcctgt tcaacgtcgg
     1801 catgacgatg ttcaaggcga agaactggac ggcgatccag gggacgctgc tcggggggct
     1861 cgtcttcctc gcccttctgt acctcttcgg catccccttc taccggaacc tcgtcctcga
     1921 ctggtattac tggtggtggg tgatccacct ttgggtggaa ggcgcctggg aactcgtcac
     1981 cgcgtcgatc ttcgccttcg tcctgatgaa gatcaccggc gtcgagcgcg aggttgtgga
     2041 gaagtggctc tacgtcgaga tcggcctgtt cctcttcacc ggactggccg gaaccgggca
     2101 ccattactac tggatcggtg cgccgagata ctggctgtgg atcgggggag tcttctcggc
     2161 cctcgagccg ctgccgatcc tcctgatggt gatcgacacg atgcaccacg tgaaggagcg
     2221 gaagacgaag atcatcaacc ccctcacctg gacgtacgcc atcggctgcg cgatcatgca
     2281 tctcatcggc gccggcgtgt ggggattcct gcacaccctg ccgcagatca actactacac
     2341 gcacggctcc caggtcacc
//
LOCUS       DASVJL010000261         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1542262, whole genome shotgun sequence.
ACCESSION   DASVJL010000261 DASVJL010000000
VERSION     DASVJL010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542262"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VF357_05445"
     CDS             <1..497
                     /locus_tag="VF357_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX7523624.1"
                     /translation="SVPYTLRGFEVDARFNTSTVEIFHQGKRVASHARSFLKGRHTTL
                     QEHLPPSHREYREWPPSRLIAWGATIGPSTEEVVETILSAGAYPVQGYRRCLGILRLG
                     KKYGEARLESACERALALRALSYKSIESILKTGSEKLPLRKKATAPELPLFHDNIRGP
                     GYYH"
     gene            522..1289
                     /gene="istB"
                     /locus_tag="VF357_05450"
     CDS             522..1289
                     /gene="istB"
                     /locus_tag="VF357_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX7523625.1"
                     /translation="MLLHPLMEKLSTLKLVGMARALEEQIRNPEIESLSFTDRLGLLV
                     DHESVERENRRMKSRLKAARLREAAVVEDIDYRHPRGLDKALMLKLAACAFVREHLNV
                     FITGPTGAGKTYLSCALGNKACREGFSVSYVRLPRLLENLGIGRGDGRYAKILDAFAR
                     IELLILDDWAIAPLTDEQRRDLFEILEDRYGRKSTIVVSQLPVEKWHDALGEPTLADA
                     ILDRLLHNGYKIALRGESLRKKKGQQRLTEIAHCEKQ"
BASE COUNT          320 a          504 c          449 g          248 t
ORIGIN      
        1 acagcgtccc gtataccctg cgtggcttcg aggtcgacgc ccggttcaac acctccaccg
       61 tggagatctt ccatcagggg aagcgagtgg catcccacgc ccgcagcttc ctcaagggaa
      121 ggcacaccac cctgcaggaa catctgcccc cgtcccaccg cgagtaccgg gaatggcctc
      181 cctcccgcct gatcgcgtgg ggtgccacga tcggccccag taccgaagag gtggtggaga
      241 cgatcctctc cgccggggca tatcccgtcc aaggataccg ccgctgcctg ggaatcctgc
      301 gtctggggaa aaagtacgga gaggcgcggc tggagagcgc ctgtgagcgc gctctggcgc
      361 ttcgcgccct ctcgtacaag tccatcgagt cgatcctgaa aaccggctcc gagaagttgc
      421 ctctccggaa gaaagcaacc gccccggaac ttcccctgtt ccacgacaac atccgggggc
      481 ccggctacta ccactgatcc cacacgaaag gagaacaagg aatgctgctg caccccctga
      541 tggaaaagct ctccacgctg aagctcgtcg gaatggcgcg agcgctcgaa gaacagatcc
      601 gcaacccgga gatcgagtcc ctctccttta cggaccggct ggggctgctc gtcgatcacg
      661 aaagcgtgga acgcgagaac cggcggatga agagccgcct caaggcggcc cgcctgcggg
      721 aggccgccgt cgtcgaggac atcgactacc gtcatcctcg cggcctggac aaggcgctca
      781 tgctcaaact cgccgcctgc gccttcgtcc gggagcacct caacgtcttc atcaccggac
      841 ccacgggagc gggcaagacg tatctgtcgt gcgcgctggg gaacaaggcg tgccgcgagg
      901 ggttcagcgt ctcgtacgtc cgcctcccgc ggttgctcga aaacctgggc atcgggcgcg
      961 gcgacgggcg atacgccaag atcctcgacg ccttcgcgcg catagaactc ctgatccttg
     1021 atgattgggc gatcgcgccc ctgaccgacg agcagcgacg cgacctgttc gaaatcctcg
     1081 aagaccgata tggcagaaag tccaccatcg tcgtcagcca acttccggtg gagaagtggc
     1141 acgatgccct cggagaaccc accctggcgg acgctatcct cgatcggctt cttcataacg
     1201 gctacaaaat cgccctcagg ggagaatcct tgcggaagaa gaaaggacaa caaaggttga
     1261 cggaaatcgc ccactgtgag aaacaataac ccccggcgtc gctgacgctc cgagggtggc
     1321 cggaatcctc tcggaacgat ggccgacttc gtctcggttc aagtggccga aatcgtcgga
     1381 atgcgcatcc cctgcccccc tccaggagcc gatccaacct gaggtcaatg cgaatggaaa
     1441 agaaggcctt caggggtctt ccgggccgga ggcccgggag accccgcagg gctctccttt
     1501 acttgttctt aactacggag a
//
LOCUS       DASVJL010000262         8374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_40224, whole genome shotgun sequence.
ACCESSION   DASVJL010000262 DASVJL010000000
VERSION     DASVJL010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40224"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..477
                     /locus_tag="VF357_05455"
     CDS             148..477
                     /locus_tag="VF357_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523626.1"
                     /translation="MKRFAIASMLFVVAVAAAGFLAPGAASAEEIKLVGVITKIEIAG
                     PDATTASATLKDNKTGDLVTVIVNDALTLDKFKDHRIVTGDEIRLKYEVKDGKNVSKY
                     FRKTAGC"
     gene            575..2773
                     /locus_tag="VF357_05460"
     CDS             575..2773
                     /locus_tag="VF357_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7523627.1"
                     /translation="MTIELSRRTFLKVTGTAAAGLSIDAAMPEPFLSWAETAPEELKQ
                     VPTFCELCFWNCGLIAKVQNGKVVKIDGNPLGERGRGRLCGRGNAALGALYDPDRLKV
                     PLINTGKRGAPIWKRASWDEALGLVAGKLQTIKEKHGPETVALFSHGTGGSFWKHLLK
                     AYGSGTFAAPSFAQCRGPRDVGFTLTFGSDVGSPEYYDFSESRHIVLIGSHLGENAHN
                     SQVQDIVQGVSKGARLTVVDPRLSNIAAKADRWLAIKPATDMALLLAWIRIIVNEELF
                     DGDYLKAYATGLPELKAAVQEYTPEWAARETDLPAETIVSVARELAAHKPNVIVSGGR
                     FTAWYGDDTQRSRAIAILNALLGAWGRPGGIYLPAGGDVPEYPGLPKYPPHRDDLPAL
                     DAAYPFAMAQTTTSIRQASITGKPHPIKGWVVYGCNLIKTLPDRAETLRAIDNLDLLV
                     AIDIMPADITQWADVVLPECTYLERYDTLNLGKFKEFEVSIRQPAVEPLFESKPSWWI
                     TRELAVRMGLASYFPWKDGEEYLRGRCRTGNIDFEELRRKGVLTFSEESHPYISAQTK
                     PEFGTPSGKIELWSKQLSEKGFDPVPKYTRHAQPPAGYFRVLFGRSPLHTFSRTTNNF
                     LLTDLIPENVFWISKKKALELGLGDGESVRLENPAGYKSPGEIKVRHTQRLREDCIYM
                     VHGFGVHARELTRANGKGIGDEEMLGGHAIDPIMGGTAMRSNFVKIVKKG"
     gene            2777..3931
                     /gene="nrfD"
                     /locus_tag="VF357_05465"
     CDS             2777..3931
                     /gene="nrfD"
                     /locus_tag="VF357_05465"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX7523628.1"
                     /translation="MRFDGTILPDRTRLGEAITDILKGKGGGPLYLSLLGLLAGFIAI
                     GCIFLLGHAHTTNTSDLVPWGIQITTYVYFVLVSTGCTFVNFFGEIFHEEEYRPYASR
                     VMFLAIVTALAAFISLATEMGRIDRMYYFFVSPNPASPMFWMSVWYVADVFVIVLEYV
                     NIRRNRHSRKVMWAAFFVAVATYGTLGSLFGAVSSRAYYYSAMLPVYFLFVAFLSGSA
                     LAAIVAAVERKKAGGKGSPLPAPFPTFLKIGLGLALFATFWRLMIGLAGHVTGSEVFR
                     LTLFDTLFFGIFLGIIVPFLLLLVVRGPGGLIFTGLWILVTQFKARIDLVVGGFQVPV
                     FRAYDIPEVVRYTPSVFEALVVVASVSLVVFFYLVCDRSGLFETIPGKDA"
     gene            3936..4646
                     /locus_tag="VF357_05470"
     CDS             3936..4646
                     /locus_tag="VF357_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7523629.1"
                     /translation="MEPREDILEIMREDLRRALLKKREQRRWAMAIDLKKCIGCHACT
                     TGCMSENLTPPGMHYRPVREEEIGEFPNVSLRFLPRPCMQCDAPTCTPVCPVKATYKR
                     DDGIVAIDYRKCIGCRYCLVACPYGARSADVGRYYTGEMKKQTYEVENSGEYGKVFKR
                     KTHWHSPIGNARKCHFCIHRVEKGELTRCTTTCLGHATYFGDLSDPKSLISKVVAKAN
                     TFRYREELGTKPRVIYLV"
     gene            4689..4907
                     /locus_tag="VF357_05475"
     CDS             4689..4907
                     /locus_tag="VF357_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523630.1"
                     /translation="MRKLTALLIAALFALGLSFYAFAAEVKKDEPAAKEATTLKKDNT
                     VEKEKKTGKEGKKDDAKPKKPKKESSGC"
     gene            4936..6321
                     /locus_tag="VF357_05480"
     CDS             4936..6321
                     /locus_tag="VF357_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7523631.1"
                     /translation="MQHSRSMSILLAAFLLLLSAATGMPAKPAKAEPPAGAKMCLICH
                     KPAPGNLLGNWEVVAMKASSIQMKIDDRAEVFRFDPSTVQVLNAREKGDIEKMLRSIK
                     NGAAVRVSFAEKDGIRYARVVSVMPPVKLPEKDRISLADLEMLVAHGRESGDYTLIDS
                     RPARLFKEGAIPTALSLPFPEFEKNHDRLPADKNRLVIFYCSGPACTMSPNSQARTKK
                     LGYTNTKVFVGGMPEWLGKNYAVTTARSFLEAYKDLSYVLLDAREPGVAEKGFAKGAV
                     TFPAADNAAIQSLPKKALNAPIIVYDEDGKGNGNKVASAIVKAGYANTLLVSDGFLGM
                     QQAGLSMEKGKLPTVIAYVPKPKPGEFPIEQFRKITTSGVPADTVLLDVRSREEAKEE
                     TIKGALNIPADDLEKDFAKLPKDKKIITLCNTGTRAEMAYHILKAKGFPKVFFLNAMI
                     DIEDGKPTIAD"
     gene            6387..8210
                     /gene="extM"
                     /locus_tag="VF357_05485"
     CDS             6387..8210
                     /gene="extM"
                     /locus_tag="VF357_05485"
                     /inference="COORDINATES: protein motif:HMM:NF040943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon c-type
                     cytochrome ExtM"
                     /protein_id="HEX7523632.1"
                     /translation="MLLLLLVFGLAAGCAHSTQDDSCLACHRGIETASPDHPGCIGCH
                     GGDGKRPTAEAAHRGMRGPRNPADPGHWEEGCGRCHPYQLGRVRSGLMFTNAGMIRNI
                     QRTWEGEGGGRYAARGGDAIDADGKPIRYRPVAELENLSGELYRKFCSRCHLGIEIGD
                     AYGSSHAAGCAACHFPFNETATYEGKDPTVRGKAPYPASHRLEPLPPTEVCARCHNRS
                     GRIALSYQGLYDGNNGLVPTRDGEPGPVTLSGGRNAVHITPDVHAAAGMECIDCHTSR
                     DVMGDGYSYRNMYHQVEIACEDCHGGAEAPRYRVIARENDEAVRESRRYRMPMRPGMT
                     MVLTARGRSYSNVFVREGEVYVLGKRSGKLFRSKWITGTPEHAVVGHERLECYACHSL
                     TVVQCYGCHTRYDRREKSTDFIKGVETPGAFSETEDYRTLFPFPLAVNQRGRISPVTP
                     GCQTFVTAVDEQGTVVRDEEVARFRGKRQFRFSPIYSHNTGKRAVGCRECHANPAFLG
                     FGQHVVEGGSIAGTLLCEKSPDRPLDGFLILEDGRVRAVAAITRENSRPLDGAEIRRV
                     FAANLCITCHATARDKDRIYGRRLDYGALRDDVHRRLLAGP"
     gene            8164..>8374
                     /locus_tag="VF357_05490"
     CDS             8164..>8374
                     /locus_tag="VF357_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523633.1"
                     /translation="MRCATTCIAAFWLVLSVAPAMAAESCTVCHRVERAGLHRKLACS
                     ACHGNEPADGNGPAATLRKAAPCDRC"
BASE COUNT         1640 a         2728 c         2616 g         1390 t
ORIGIN      
        1 gcgatctccg aagtcgcgtc caacccccag ctgctgacgc cgatcaagag cgcgaaggac
       61 ctgtacgcaa cactggaagt gaaattctag gacacgaggg aagagggggg gtgccccccc
      121 tctgatcctc cggaaaggga gagattcatg aaacggttcg cgatcgcatc catgcttttc
      181 gttgtggcgg ttgccgcggc ggggttcctt gcccccggtg cggcgtccgc ggaggagatc
      241 aaactggtcg gggtcatcac gaagatcgag atcgcgggac ccgacgccac gaccgcgtcg
      301 gcgacgctca aggataacaa gaccggggac ctcgtcacgg tgatcgtcaa cgacgccctg
      361 acgctcgaca agttcaagga tcaccggatc gtcacgggcg acgagatccg gctgaagtac
      421 gaggtcaagg acggcaagaa cgtcagcaag tatttccgca agaccgcggg atgttgacga
      481 cgccggccgc gaacggaaca ggaagaagcg aggctgacga gcgccgcgat ctgcttcgac
      541 gagatggcgg ggcagaagaa agaggattgg tgccgtgacc atcgaattgt ctcgaagaac
      601 attcctcaag gtgaccggca ccgctgcggc cggcctctcg atcgacgccg ccatgcccga
      661 accgttcctc tcgtgggccg aaacggcgcc ggaggaactg aaacaggtgc ccaccttctg
      721 cgaactctgc ttctggaact gcgggttgat cgccaaggtg cagaacggca aggtcgtcaa
      781 gatcgacggc aacccgttgg gcgagcgagg gcgcgggcga ctctgcggcc gggggaacgc
      841 ggccctgggc gccctctacg atccggaccg gcttaaggtt ccgctgatca acaccgggaa
      901 gcggggcgcg ccgatatgga agcgggccag ctgggacgag gcgttggggc tggtcgccgg
      961 gaaactgcag accatcaagg aaaaacacgg tccggagacc gtggccctct tctcccacgg
     1021 aaccggcggc tccttctgga agcacctgct gaaagcatac ggcagcggaa cttttgccgc
     1081 cccctccttt gcccaatgcc gcgggccgcg ggacgtgggt ttcaccctga ccttcggttc
     1141 cgatgtcggg tcgccggaat attacgactt cagcgagagc cgccatatcg tgctcatcgg
     1201 ctcccatctc ggagaaaacg cccacaacag ccaggtccag gatatcgtcc agggcgtttc
     1261 gaaaggggcc cggctgaccg ttgtcgaccc gcgcctgtcg aacatcgccg cgaaggccga
     1321 ccgctggctc gccatcaaac cggccaccga catggcgctc cttctcgcct ggattcgaat
     1381 catcgtgaat gaggagcttt tcgacggcga ctatctgaag gcgtacgcca ccggcctgcc
     1441 cgagctcaag gcggccgtgc aggagtacac ccccgaatgg gccgcccggg agaccgatct
     1501 cccggcggag accatcgttt cggtggcccg ggagttggcg gcgcacaagc ccaacgtcat
     1561 cgtttccgga ggccgcttca ccgcctggta cggcgacgat acccaacgca gccgcgccat
     1621 cgccatcctg aacgccctcc tcggcgcctg ggggcgtccg ggaggcatct atctccccgc
     1681 cggaggcgat gtcccggaat acccggggct tccgaagtac ccgccccacc gggacgatct
     1741 tcccgcgctg gatgcggcct atccgtttgc catggcgcag accaccacct ccatccggca
     1801 agcatcgatc accggaaaac cgcacccgat caagggctgg gtggtctacg gctgcaacct
     1861 gatcaagacg ctcccggacc gggccgagac cctgcgcgcg atcgacaacc tcgacctgct
     1921 ggtagccatc gatatcatgc ctgccgacat cacccagtgg gccgacgtcg tcctcccgga
     1981 atgcacctat cttgagcggt acgacacctt gaacctcggc aagttcaagg agttcgaggt
     2041 gtccatccgg caacctgccg tggaacctct cttcgagagc aagccctcct ggtggatcac
     2101 cagggaactg gcggtcagga tgggccttgc ctcgtacttc ccgtggaagg atggggaaga
     2161 gtacctgcgc ggccgctgcc gcacgggaaa catcgacttc gaggaattgc ggcggaaggg
     2221 agtcctcacc ttcagcgagg aatcgcaccc ctacatctcc gcgcagacaa agccggaatt
     2281 cggcaccccc tcggggaaga tcgagctctg gtccaagcaa ctttcggaaa aagggttcga
     2341 ccccgtcccg aaatatacga ggcatgcaca gcccccggcc ggttatttcc gggtcctgtt
     2401 cggccgttcg ccgcttcaca ccttctcccg gaccacgaac aatttcctcc tcacggacct
     2461 gatcccggag aacgttttct ggatcagcaa gaaaaaggcg ctggaactgg ggctcgggga
     2521 cggggagtcc gtccggctgg agaatccggc aggctataag tccccggggg agatcaaggt
     2581 acgccacacc caacgactcc gcgaagactg catctacatg gtccacggct tcggcgtcca
     2641 cgcccgggaa ctcacgaggg ccaacggcaa gggaatcggc gatgaggaga tgctcggcgg
     2701 gcacgccatc gaccccatca tgggagggac cgccatgagg tccaacttcg tcaagatcgt
     2761 gaaaaagggg taaccgatgc gattcgacgg aaccatcctt ccggatcgga cccggctggg
     2821 ggaggccatc accgatatcc tcaaggggaa ggggggaggg ccgctctacc tttcgctcct
     2881 tggactgttg gccgggttca tcgccatcgg gtgcatcttc ctcctggggc acgcgcacac
     2941 gacgaacacc tccgacctgg tcccgtgggg gatccagatc accacctacg tctacttcgt
     3001 cctggtcagc acgggctgca ccttcgtcaa cttcttcggc gagatcttcc acgaggagga
     3061 atacaggccc tacgcgtccc gcgtcatgtt cctcgcgatc gtgacggccc tggcggcatt
     3121 catctccctg gccaccgaaa tgggccggat cgaccggatg tactacttct ttgtctcgcc
     3181 caaccccgcc tcgcccatgt tctggatgtc ggtctggtac gtcgccgacg ttttcgtcat
     3241 tgtcctcgaa tacgtcaaca tccggcggaa ccggcactcc cgcaaagtca tgtgggccgc
     3301 cttcttcgtc gccgtcgcca cctatggcac gctggggagc ctcttcgggg cggtcagctc
     3361 gcgcgcctac tactacagcg ccatgctgcc ggtctacttc ctcttcgtcg ccttcctctc
     3421 cggctccgcg ctggccgcca tcgtcgccgc ggtcgaacgg aagaaggcgg ggggaaaggg
     3481 gtcgcccctc ccggcccctt tccctacttt cctgaagatc ggcctggggc ttgcgctctt
     3541 tgcaaccttc tggcgcctga tgatcggcct ggcggggcac gttaccggtt ccgaggtctt
     3601 tcggctcacc cttttcgaca ccctcttctt cggcatcttt ctcggcatca tcgtgccgtt
     3661 cctcctgctc ctggtggtcc gcggccccgg cgggctgatc ttcaccggcc tctggatcct
     3721 ggtcacccag ttcaaggcgc ggatcgatct ggtggtcggc ggctttcaag tgcccgtctt
     3781 ccgcgcctac gacatccccg aggtggtccg ctacaccccg tcggtcttcg aggcgctggt
     3841 cgtggtcgcc tcggtgtcgc tggttgtctt cttctatctg gtgtgcgatc gttccggcct
     3901 gttcgaaacg atccccggaa aggatgcctg attcgatgga accccgggaa gacatactgg
     3961 agatcatgcg ggaggacctg aggcgggctc ttctgaaaaa gcgtgaacag cgccgctggg
     4021 caatggccat cgacctgaaa aagtgcatcg gctgccacgc ctgcaccacc ggatgcatgt
     4081 ccgagaacct gacccccccg ggaatgcatt accggccggt gcgggaggag gagatcggcg
     4141 agttcccgaa tgtatcgctg cggttcctgc cgcgtccctg catgcagtgc gacgccccca
     4201 cctgcacccc ggtctgcccg gtgaaggcga cctacaagcg ggacgacggg atcgtcgcga
     4261 tcgactacag gaagtgcatc ggctgccgat attgcctggt cgcctgcccc tacggcgccc
     4321 gctccgccga cgtcggccgc tactacaccg gagagatgaa gaaacagacc tacgaggtgg
     4381 agaattccgg tgagtacggc aaggtcttca agcgaaaaac ccattggcac tcccccatcg
     4441 gcaacgcccg gaagtgccac ttctgcatcc accgggtgga gaagggggag ttgacccgct
     4501 gcaccaccac ctgcctgggg cacgccacct acttcggcga cctgagcgac ccgaagagcc
     4561 tgatcagcaa ggtggttgcg aaggcgaaca cgttccggta cagggaagag ctggggacga
     4621 agccgagggt gatctacctc gtctgatccg cgttcggcgg aaccaaataa taatcgggag
     4681 gaaaaccgat gagaaagctc accgctctgt tgatcgcagc actgttcgcc ctgggactgt
     4741 ccttctacgc cttcgccgcg gaggtgaaaa aggacgagcc ggccgcgaaa gaggcgacga
     4801 ccttgaaaaa ggacaacacg gtcgaaaaag aaaagaagac cggaaaagag ggcaaaaagg
     4861 acgatgccaa gcccaagaag ccgaagaagg agtcttccgg ctgctagcgg cgtccgtcac
     4921 caaaggaggt ccaacatgca gcattcaaga tcgatgagta ttctcctcgc cgctttcctt
     4981 ctgctccttt ccgccgccac gggcatgccg gcgaaaccgg caaaggcgga acccccggcc
     5041 ggagccaaga tgtgcctgat ctgccacaaa cccgcaccgg gcaacctgct cgggaactgg
     5101 gaggtcgtcg ccatgaaggc gtcgtcgatc cagatgaaga tcgacgaccg cgcggaagtg
     5161 ttccggttcg acccgtccac ggtgcaggtg ctgaacgccc gcgagaaggg cgatatcgag
     5221 aagatgcttc ggagcatcaa gaacggggcg gcggtccggg tctcgttcgc cgaaaaggac
     5281 ggaatccgat acgccagggt ggtttccgtc atgccgccgg tcaagcttcc ggagaaggac
     5341 aggatctccc ttgccgattt ggagatgctg gtcgcccacg gccgcgagtc cggggactac
     5401 accctgatcg actcccgtcc ggcccgtctc ttcaaggagg gggccatccc gacagccctc
     5461 agccttccct tcccggaatt cgaaaagaac catgatcggc ttcccgccga caagaaccgc
     5521 cttgtcatct tctactgctc cggccccgcc tgcaccatga gccccaactc acaggcaagg
     5581 accaagaaac tcggctacac caacaccaag gtattcgtcg gggggatgcc ggaatggctc
     5641 ggcaagaact acgcggtgac gaccgcacgg tcgttcctgg aggcgtacaa ggacctctcc
     5701 tacgtcctcc tcgacgcccg cgagccgggc gtggcggaaa agggattcgc caagggagcg
     5761 gtaaccttcc cggccgcgga taacgcggcc atccaatcgc tcccaaagaa agcgctgaat
     5821 gcgccgatca tcgtctatga cgaggacggg aaggggaacg gcaacaaggt cgcctcggcc
     5881 atcgtcaagg ccggctacgc caacaccctc ttggtgagcg acggcttcct cgggatgcag
     5941 caggccggtc tttcgatgga gaaggggaag ctgccgaccg tgatcgccta cgtccccaag
     6001 cccaagccgg gagaatttcc gatcgagcag ttccggaaaa taaccacctc cggcgttccg
     6061 gccgacacgg ttctcctcga tgtccggagc agggaggaag cgaaagagga aaccatcaag
     6121 ggggcgctga acatcccggc cgacgatctt gaaaaagatt tcgccaaact gccgaaggac
     6181 aagaagatca tcacgttgtg caataccggc acccgcgcgg agatggccta ccatatcctc
     6241 aaggccaagg ggttccccaa ggtcttcttc ctgaacgcga tgatcgatat cgaggacggc
     6301 aaaccgacga tcgccgattg aaatccgttt ccgtaagatg gggacccccc gggagagccg
     6361 ctcccggggg attccgtccc ctcatcatgc tcctgctcct cctggttttc gggttggccg
     6421 ccggatgcgc ccactccaca caggacgact cgtgcctcgc ctgtcatcgg ggaatcgaga
     6481 cggcctcgcc cgaccacccc ggctgcatcg gttgccacgg cggcgacggg aaacggccca
     6541 cggcggaagc cgcccaccgc gggatgcggg gcccccgcaa cccggcggat cccgggcatt
     6601 gggaagaggg atgcggccgc tgtcacccat accagctggg acgggtgcga agcggcctga
     6661 tgttcacgaa cgccgggatg atccggaaca tccagcggac ctgggaaggg gagggcggcg
     6721 ggcgctacgc ggcccgcggc ggcgacgcga tcgacgccga cggaaagccg atccggtacc
     6781 ggccggtcgc ggaactggaa aatctttcgg gcgaactcta ccggaaattc tgctcccgct
     6841 gccacctggg gatcgagatc ggtgacgcct acggatccag ccacgccgcc ggctgcgccg
     6901 cctgccactt cccgttcaac gaaaccgcga cctacgaggg gaaggacccg acggtgcgcg
     6961 gaaaggcccc gtatcccgcc agccatcggc tggagccgct gcctccgacc gaggtctgcg
     7021 cccgctgcca caaccggagc ggccggatcg ccctctccta ccaggggctc tatgacggca
     7081 acaatggcct ggtccccacg agggacgggg aacccggccc ggtgacgctg agcggaggcc
     7141 ggaacgccgt ccacattacg ccggatgtcc atgcggcggc ggggatggag tgcatcgact
     7201 gccacacctc ccgggacgtc atgggggacg gctacagcta ccggaacatg taccaccagg
     7261 tcgagatcgc ctgcgaggat tgccacggcg gcgccgaggc gcccagatac cgtgtcatcg
     7321 cgcgagaaaa cgacgaggcg gtccgggaat cccgtcgtta ccggatgccg atgcgccccg
     7381 gcatgacgat ggtcctgacg gcccgggggc gctcctactc caatgtcttc gtccgggaag
     7441 gcgaggtcta cgtactggga aagcggagcg ggaagctctt ccggagcaaa tggatcaccg
     7501 gcacaccgga gcatgccgtc gtcggccacg agcggctgga gtgctacgcc tgtcattctc
     7561 tcaccgtcgt ccagtgctac gggtgccaca cccgctacga tcgccgcgag aaaagcaccg
     7621 acttcatcaa gggggtggag acaccggggg ccttctccga aaccgaggat taccggacgc
     7681 tctttccctt tcccctggcg gtgaaccagc ggggacggat ctccccggtc acccccgggt
     7741 gccagacctt cgtgacggcc gttgatgagc aggggacggt ggtccgggac gaggaggtgg
     7801 cccgtttccg ggggaagcgg cagttccgct tttcgccgat ctactcgcac aacaccggaa
     7861 agagagcggt gggatgccgc gagtgccacg caaaccctgc cttcctcggc ttcgggcagc
     7921 acgtggtgga gggtgggagc atcgccggta cgctcctgtg cgagaaatcg cccgaccggc
     7981 cgctggacgg cttcctgatc ctggaggacg gcagggtccg cgccgtagcc gcgatcacca
     8041 gggagaattc ccgcccgctg gacggagccg agatccggcg ggtcttcgcg gcaaaccttt
     8101 gcatcacctg ccacgccacg gcgcgggaca aggaccgcat ctacgggagg aggcttgact
     8161 atggtgcgtt gcgcgacgac gtgcatcgcc gccttctggc tggtccttag cgtcgctccc
     8221 gccatggcgg cggagagctg cacggtatgc caccgtgtcg aacgggccgg ccttcaccga
     8281 aaacttgcct gttcggcctg ccacgggaac gaaccggcgg acggaaacgg tccggccgca
     8341 accttgcgga aggccgcgcc gtgcgaccgc tgcc
//
LOCUS       DASVJL010000263         9253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1634849, whole genome shotgun sequence.
ACCESSION   DASVJL010000263 DASVJL010000000
VERSION     DASVJL010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634849"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /gene="glgP"
                     /locus_tag="VF357_05495"
     CDS             complement(<1..803)
                     /gene="glgP"
                     /locus_tag="VF357_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX7523634.1"
                     /translation="MVDTTSLDEVPMRIRHFHVRPNIPKELASLEEIARNLWFSWSWE
                     AVQLFIRLNPVLWEQAYQNPVHMLGSLPEADLEAAAKDESFVANVERVSRSFLEYRKR
                     ASWFQEVHFADAGARVAYFSCEYGIDEGLPIYSGGLGVLSGDHLKSASDLGIPLVGVG
                     LLYQKGYFRQVLSLDGWQKELYPDNDWYNMPVTMEMAADGRPLEIAVNVGGEMVRARI
                     WRVDVGRTPLYLLDSNIKANSAWSREITSTLYGGDRDMRIRQEILLGVGG"
     gene            880..2814
                     /locus_tag="VF357_05500"
     CDS             880..2814
                     /locus_tag="VF357_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7523635.1"
                     /translation="MNVELPISLPDVEEIDRPAASRILATLIEASPLAIVTFDPEGVV
                     TMWNPAAERIFGWSENEALGTRLPFVPAEKQEEFLALRRRALLGEVFTEPELHRRRAD
                     GSPIVVSVSTSPLRRTDGTIYGIMSILMDVTEQKAAEESQARLTMAVAQAGESIVITD
                     TRGTIQYVNPAFERITGYDRMEVIGRTPSILRSGRHDAAFYRNLWETLRRGEVWRGTF
                     LDKRKDGTLYEEDAVISPVRDPSGQVVNYVAVKRDVTDVRRMEERLRQSQKMEAVGRL
                     AGGVAHDFNNLLTAISGYSDLLIHRLPDYSTLRRDVEEIRKAGDRAAVLTRQLLAFSR
                     RQVLQPKVLDLNAVVTNMEQMLRRLIGEDIELSTDLSPSLSRVKADPGQIEQVIVNLA
                     VNARDAMSDGGRITIATADAELSPAYAAAYSEVRPGPHVLLSVADTGQGMSDETQAHL
                     FEPFFTTKERGIGTGLGLAMVYGIVQQSGGHIRVNSAADRGSTFLIYLPRVEAPDDGE
                     QGTDRPLLPHPSPGTETILLAEDEEVVRRLAREILSGNGYKVLEAGDGREALLLSEAH
                     RGEIHLLLTDVVMPKMSGRELTDRIRLQRPDLRILYMSGYTDDAILRHGVLVDGIPFL
                     QKPFTPEELARKVREVLDSA"
     gene            2925..4901
                     /locus_tag="VF357_05505"
     CDS             2925..4901
                     /locus_tag="VF357_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX7523636.1"
                     /translation="MPSALLYVLASAFLVPGVLALLIRDPRRSRVVGFGGGAVASFLG
                     LVATLAGSMREAPMRIPLPAPVPWAASELFLDGLSAFFLALVFFIGFLVSVYAVGYMK
                     EFDAQRRGQAAPLLLNLLLLSMAFVVSAGDMLSFLLGWEAMSLASYFLVVLNHEKQEA
                     RQAGLIYLIATHIGTGGILLAFLFLEQTTGSFSFNGFARVPISDPAILSATFGFALLG
                     FGTKAGIFPFHIWLPYAHPEAPSPVSAVMSGVMIKMGIYGIVRFLFYLLPSPPASFGA
                     TLLALGLLSGVFGVLYAIVQHDLKRLLAFHSVENIGIILIGIGAASLCQATGRPDAAR
                     LLLVGGLFHVLNHATFKALLFLGAGAVLHGAHTRNIEKLGGLMRGMPLTGAAFLAGSL
                     AISAVPPFNGFASEFAIYRGLWAAAGGAGNGGVFLAAMAGLALIGGLAVVCFVKVVGT
                     VFLGNARSAQASAARDPSVAMEFPMGTLALFCLGLGVFPYLVLDRLMEITSFLPSASM
                     PAGVAVPAAGGKPFTVAIALAIAIGGLWLLRTFLLRRNGQRAGGTWGCGFPYATPRMQ
                     YTASSFADPILHVFQGILRPHTTRTERLSGRFLLEVEHETHFHDLLENGLYLPVYRLF
                     VQAAFQARRLHPGYIHLYLGYILGAIVLLLVFFH"
     gene            4917..5819
                     /locus_tag="VF357_05510"
     CDS             4917..5819
                     /locus_tag="VF357_05510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX7523637.1"
                     /translation="MMVAAHIALFVLLGPLLVGVVRKTKAFLSCRQGAPVFQPYLDLW
                     KLFQRGIVRSTTASWVFDLSAPVVLGASLVLAAATPLWSSDVPLPMHFILFLYLMALP
                     RFFQTLAGLDTGSAFGGLGSSREVAVSAMAEPALILSFCGLAYLGGADTVSGMVAGVP
                     PGRVPWRPEMILLAGTMFLLLLAENARIPVDDPATHLELTMIHEAMVLDHAGVDLAMI
                     QLASGVRLVLFSALISGTLFPETFSLPPFLAIPAAVVKVVLSGALIGATEAGMARMRL
                     SRIASYLLVATVLAAIALAVRFAG"
     gene            5830..6456
                     /locus_tag="VF357_05515"
     CDS             5830..6456
                     /locus_tag="VF357_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523638.1"
                     /translation="MMGTVTVLLLLLGLHMAMTERIPTAIRSLAAQGVLLALLPFLAS
                     PRPLWRAAALGLATVAVKSVAIPVILTHAVSRLEIKRETESLVSTKTSVLLTGVLALS
                     AFVACARFGLSGQDQLGAAAGLTLLFSGLLIMTTRVKAITQVIGFVVLENGIFLFGAA
                     SGSEFTLPIELGIFLDLFVAVFLMGILMLKIQRTFTHMDVRKIHTLKD"
     gene            6459..7874
                     /locus_tag="VF357_05520"
     CDS             6459..7874
                     /locus_tag="VF357_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX7523639.1"
                     /translation="MIPLLLLAPLTGVILALLSRAPWQAALGTVVGAAGLLSCAVIQG
                     VSPRVAAWSDLLRLDELGSYFLLIVAGIHFLCSVYAYGYFRRELEGGHIGPAKIQMYH
                     VWTNLFVLSMVVVVCSHHLALQWVAVEATTLFSAPLIYLHGDRRGMEAAWKYLLICSV
                     GIAFAFLGIIFLSRAAGDVPLTYTALPLAARQLDPRFLRLAFLFFLVGYGTKMGLAPM
                     HTWLPDAHSQAPAPASAMLSGVLLNCALLSILRIAPAIPGAYASPLLILFGILSIAVP
                     ALLVAVQRDIKRLLAFSSIENMGIVVLGLGFGGAARPAALLHVLNHSVAKVLLFLVAG
                     DLILRFGTQKIRHIHGILRESPLRGVLLFGGILAITGSPPFGAFWSEFGILVGGIAGG
                     HLAVSLAYLVLLAILFGSFLMYGSDMVFGEGVPRKLAAPELWCLLPACVLLLASLWLG
                     LHVPASLYKTLERLSTVVAFS"
     gene            7871..>9253
                     /locus_tag="VF357_05525"
     CDS             7871..>9253
                     /locus_tag="VF357_05525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX7523640.1"
                     /translation="MTGERNKSMRCVHTFDLGLSAPLAEEGFRAFLGETSTGGDRLLA
                     LFAEEPSPGRFLLHAVAGGSAPGRFVHACVEASPRSFRTFTDVCPGTHWFEREIYDLF
                     GLVPEGHPELNPLRFHEGWPKKFFPLRSADAPTPDARRGYRFLLVRGEGIHEVAVGPV
                     HAGIIEPGHFRFSCAGETIQNLEIRLGYQHKGIESLFPGGPGMGKILLSESISGDTAV
                     GHSTACARLLEAITGKPADPSAERWRAAALEIERISNHCGDLGGLSTDIGYLFGASHF
                     GRLRGAALNLLLKITGNRFGRGLNRIGGIGPRLPGIAPEDLMAELGRLRAEVENVGTT
                     MLDASSAVARMEGTGRLLPRTAKTLQLVGVAARASGRDVDARRDFPSPAYVESPPAKV
                     LLDTGDVMARASVRFMEMRASFDWLSGILAAAMPPRTIAAEGVVPAAGRRLVVSVEEG
                     WRGEIVHAAIV"
BASE COUNT         1296 a         3234 c         2993 g         1730 t
ORIGIN      
        1 ccgccgacgc cgagcaggat ctcctgccgg atgcgcatgt cccggtcccc cccgtacagg
       61 gtggaggtga tctcgcggga ccacgcggag ttcgccttga tgttgctgtc gagcaggtac
      121 aggggggtgc ggccgacgtc cacccgccag atgcgcgccc tcaccatttc tccgccgacg
      181 ttcaccgcga tctccagggg gcggccatcc gccgccatct ccatcgtcac cggcatgttg
      241 taccagtcgt tgtccgggta cagctccttc tgccacccgt ccagcgacag cacctggcgg
      301 aaatatccct tctggtacag gagcccgacg ccgaccagcg ggatcccgag gtcggaagcc
      361 gatttcaggt ggtcgcccga gaggactccg agccccccgg agtagatcgg gagtccctcg
      421 tcgatcccgt actcgcaaga gaaataggcc acccgggcgc cggcgtcagc gaagtggacc
      481 tcctggaacc acgacgccct tttccggtac tccaggaacg accgggacac ccgctcgacg
      541 ttcgcgacga agctctcgtc cttcgcggcg gcttcgaggt ccgcctccgg cagcgatccg
      601 agcatatgca ccggattctg gtacgcctgc tcccatagaa cgggattcag ccggatgaag
      661 agctgcaccg cctcccagct ccacgagaac cagaggttcc gggcgatctc ctcgagcgaa
      721 gcgagttcct tcgggatgtt cggtcggacg tggaaatgac gtattcgcat gggtacctcg
      781 tcgagggaag tcgtgtccac catgaaacat cagccggggg ggcaagtcaa ttcggcgggg
      841 tgttaaagat tccccggggt tttgccgaaa cgatcattga tgaacgtgga actgcccata
      901 tctttacccg acgtcgagga gatcgaccgg ccggcggcgt cccggatcct cgccaccctc
      961 atcgaggcgt ccccgctggc gattgtcacg ttcgatcccg aaggcgtcgt cacgatgtgg
     1021 aacccggcgg ccgagcggat cttcggatgg tccgagaacg aggccctcgg gacgcgcctc
     1081 cccttcgtcc cggccgagaa gcaggaggag ttcctcgcgc tccgccgacg cgccctcctc
     1141 ggggaggtgt tcacggaacc cgagctgcac cggcggcggg ccgacggatc tccgatcgtc
     1201 gtaagcgtgt cgacctcccc ccttcgccgg acggacggga ccatctacgg gatcatgtcg
     1261 atcctgatgg acgtcaccga gcagaaggcg gccgaagagt cacaggcccg gctgacgatg
     1321 gcggtcgcgc aggcgggcga gagcatcgtc atcaccgaca cccgtggcac gatccagtac
     1381 gtcaaccctg ccttcgagcg gatcaccggg tacgatcgga tggaagtcat cgggcggacc
     1441 ccgagtatcc tgaggagcgg ccggcacgac gcggcgttct acaggaactt gtgggagacc
     1501 ctccgccgcg gcgaggtatg gcgggggacg ttcctcgaca agaggaagga cggcaccctc
     1561 tacgaggagg atgccgtcat ctccccggtg cgggacccct cgggccaggt tgtgaactac
     1621 gtcgccgtca agcgcgacgt cacggatgtc cgtcggatgg aggaacggct gcgccagtcc
     1681 cagaagatgg aggccgtcgg aaggcttgcc gggggcgtcg cccacgactt caacaacctg
     1741 ctgaccgcca tctccggcta ctccgacctt ctgatccacc gcctccccga ctactcgacg
     1801 cttcgccggg acgtggagga gatccggaag gcgggggacc gcgccgccgt gctcacgcgc
     1861 cagctgctcg ccttcagccg tcgccaggtc ctgcagccga aggttctgga cctgaacgcc
     1921 gtggtaacga acatggaaca gatgctgcgc cgcctgatcg gcgaggacat cgagctgtcg
     1981 accgacctgt ccccttccct ctcgcgggtg aaggctgacc cggggcagat cgagcaggtg
     2041 atcgtgaacc tggcggtcaa cgcccgggac gcgatgtccg acgggggccg catcaccatc
     2101 gcgaccgccg acgcggagct gtcccccgcc tacgcggccg cctattccga agtgcgtccg
     2161 ggaccgcacg tgctcctctc cgtggcggac acggggcaag ggatgagcga cgagacccag
     2221 gcgcacctgt tcgaaccgtt tttcaccacg aaggagaggg gaatcgggac cggccttggc
     2281 ctggccatgg tctacggcat cgtgcagcag agcggcgggc acatccgggt gaacagcgcg
     2341 gccgaccgcg ggtcgacgtt cctcatttac ctgccgcggg tcgaggcccc cgatgacggc
     2401 gagcagggga cggatcgacc actcctcccc cacccttccc ctgggacgga gacgatcctc
     2461 ctggccgagg acgaagaggt ggtgcggcgg cttgcacggg agatcctgtc ggggaacggg
     2521 tacaaggtgc tcgaggccgg ggacggccgc gaggcgctgc tgctctccga agcccatcgc
     2581 ggggagatcc acctgctgct gaccgacgtg gtgatgccga agatgagcgg gagggagctc
     2641 accgatcgaa tccggctcca gcgcccggac ctccggatcc tgtacatgtc ggggtacacc
     2701 gacgatgcca tccttcgcca cggcgtcctg gtggacggga tccccttttt gcagaagccg
     2761 ttcaccccgg aagagctcgc gcggaaggtg cgcgaggtgc tggactccgc ttgaccttcc
     2821 ccgggatctc ctgtaatgtt tccctcctga taggaccgtg acctccgttt caacgcgccc
     2881 ggcccgtcgt cctccaacga tccgatgata tggagccaac cacgatgcct tcggcgttgt
     2941 tgtacgtact cgcctccgcc tttctcgtcc ccggcgtcct tgcgctcctg atccgggacc
     3001 ctcgccgctc gcgcgtcgtc ggtttcgggg ggggggccgt cgcctccttc ctcggcctgg
     3061 tcgccacgtt ggccggatcg atgagggaag ccccgatgcg catcccgctt ccggcccccg
     3121 tgccgtgggc ggcctcggaa ctattcctcg acgggctttc cgcgtttttc ctcgccctgg
     3181 tcttcttcat cgggttcctc gtctccgtgt acgccgtcgg gtatatgaag gagttcgacg
     3241 cgcaaaggag ggggcaggcc gcgccgctgc tcctgaatct gctcctgctc tccatggcct
     3301 tcgtcgtttc ggcgggggac atgctgtcgt tcctgctcgg ctgggaggcg atgagcctcg
     3361 cttcctactt cctcgtcgtc ctcaatcacg aaaagcagga ggcgcggcag gcggggctca
     3421 tctacctgat cgccacgcac atcgggacgg gcggcatcct cctggcgttt ctgttcctcg
     3481 agcagacgac gggctcgttt tccttcaacg ggttcgcccg ggtccccatc tccgacccgg
     3541 cgatcctctc cgccacgttc gggttcgccc tgctggggtt cggcacgaag gcggggatct
     3601 ttccgttcca catctggctg ccgtacgccc atccggaagc gccttcgccg gtttccgccg
     3661 tcatgtccgg cgtcatgatc aagatgggga tctacgggat tgtccggttc ctcttctacc
     3721 tgctcccctc ccctcccgct tccttcgggg cgaccttgct tgccctgggc ctgctctccg
     3781 gtgtcttcgg cgtcctctac gcgatcgtcc agcacgacct gaagcgactg ctcgccttcc
     3841 acagcgtcga gaacatcgga atcatcctga tcggcatcgg ggcggcctcc ctgtgccagg
     3901 ccaccggacg gcccgatgcc gcgcggctgc tcctcgtcgg ggggctgttc cacgtcctga
     3961 accacgcaac gttcaaggcg ctccttttcc tgggggccgg cgcggtcctc cacggcgcgc
     4021 acacgcgcaa catcgagaaa ctcggggggc tgatgcgggg gatgccgctc accggggccg
     4081 cgttcctcgc gggatccctc gccatctcgg ccgtgccgcc gttcaacggg ttcgcgagcg
     4141 agtttgccat ctaccggggg ttgtgggccg ccgcgggggg cgcgggaaac gggggagtct
     4201 tcctcgccgc gatggcgggg ctcgcgctga tcggggggtt ggcggtcgtc tgcttcgtca
     4261 aggtcgtggg gacggtcttc ctcggaaacg cgcgcagcgc ccaggcgtcc gcggcccgcg
     4321 acccgtccgt cgcgatggaa ttcccgatgg ggacgctcgc ccttttctgc ctcggtctcg
     4381 gggtcttccc ctacctcgtc ctggaccgcc tcatggagat cacctccttc ctcccgtcgg
     4441 cgagcatgcc ggcgggcgtc gcggtcccgg cggcgggcgg gaaaccgttc accgtggcga
     4501 tcgcgctggc gatcgcgatc ggggggctgt ggctcctccg gacgttcctg ctgcggcgga
     4561 acggccagcg cgcgggggga acgtgggggt gcggcttccc gtacgccacc ccgcggatgc
     4621 agtacaccgc gtcgtcgttc gccgacccga tcctgcacgt ctttcagggc atcctccgtc
     4681 cgcacacgac ccgcacggag cggctctccg gaaggttcct gctcgaggtg gagcatgaga
     4741 cgcacttcca cgacctcctc gagaacggcc tctacctgcc ggtctaccgc ctgttcgtgc
     4801 aggccgcgtt ccaggcgcgc cgcctccacc cggggtacat ccacctttac ctcggctaca
     4861 tcctcggggc catcgtgctc ctgctcgtct ttttccatta agggagaaac gctttcatga
     4921 tggtcgccgc acacatcgcg ctcttcgtcc tcctcgggcc cctccttgtc ggagtcgtcc
     4981 ggaagacgaa ggcgttcctc tcctgccggc aaggcgcgcc ggtgttccag ccatacctgg
     5041 acctttggaa actgttccaa agagggatcg tgcgcagcac gacggcgtcg tgggtgttcg
     5101 acctgtccgc cccggttgtc ctcggcgcgt cgctggtcct cgcggcggcg acccccttat
     5161 ggtccagcga cgtgccgctc ccgatgcact tcatcctctt cctctacctg atggcgctgc
     5221 cccggttctt ccagaccctg gccgggctgg acacggggag cgcgttcggg gggctgggat
     5281 ccagccggga agtggccgtt tccgcgatgg cggagccggc gctgatcctt tcgttctgcg
     5341 gcctggcgta cctcgggggg gcggacacgg tgagcgggat ggtcgcgggc gttccccccg
     5401 gaagggtgcc atggcggccg gagatgatcc tcctcgccgg cacgatgttc ctgctgctgc
     5461 tggcggaaaa cgcgcggatc cccgtggacg acccggcgac gcacctcgaa ctgacgatga
     5521 tccacgaagc gatggtgctg gaccacgcgg gggtcgatct cgcgatgatc cagctggcct
     5581 ccggcgttcg cctggtcctc ttttccgccc tcatctccgg gaccctcttc cccgagacgt
     5641 tctccctgcc gccgtttctc gcgatcccgg cggccgtcgt gaaggtcgtc ctttccgggg
     5701 cactgatcgg cgcgacggag gcggggatgg cgcggatgcg gctctcgcgg atcgcctcat
     5761 acctgctggt cgccaccgtg ctggccgcca tcgcgctcgc cgtccggttc gcggggtgag
     5821 gagggaaaga tgatgggcac cgtcaccgtt cttcttctcc tcctcggcct tcacatggcc
     5881 atgacggagc gcatccccac cgcgatccgg tcgctggccg cgcaaggagt cctgctcgcc
     5941 ctcctcccct ttctcgcgtc gcctcggccc ttgtggcgcg ccgcggcgct gggcctcgcc
     6001 accgtcgccg tgaagagcgt ggcgattccg gtgatcctca cccacgccgt ttcccgcctg
     6061 gagatcaagc gggaaacgga atcgctcgta tcgacgaaga cctccgtcct gcttacgggg
     6121 gtgctcgccc tttccgcctt cgtcgcctgc gcccggttcg ggctttccgg ccaggaccag
     6181 ctcggcgccg ccgcgggcct cacgctcctt ttttccggac tcctcataat gaccacgcgc
     6241 gtcaaggcca tcacccaggt gatcgggttc gtcgtcctcg aaaacgggat cttcctgttc
     6301 ggcgcggcgt cgggcagcga gttcacgctg ccgatcgagc tggggatctt cctcgacctg
     6361 ttcgtcgccg tcttcctgat ggggatcctg atgctcaaga tccagcggac gttcacccac
     6421 atggatgtcc ggaaaatcca cacgttgaag gattagggat gatcccgctg ctgcttctcg
     6481 ccccgttgac cggggtgatc cttgcgctcc tgtcgcgcgc cccctggcag gccgccctcg
     6541 gaaccgtcgt cggcgccgcc ggacttcttt cctgcgcggt gatccagggg gtgtcccccc
     6601 gcgtcgccgc gtggagcgat ctattgcggc tcgacgagct cggatcgtac ttcctcctga
     6661 tcgtcgccgg catccacttc ctgtgctccg tttacgcgta cgggtatttc cggcgggagc
     6721 tcgagggagg gcacatcggc ccggccaaga tacagatgta tcacgtgtgg accaacctgt
     6781 tcgtcctctc gatggtcgtg gtcgtctgct cccaccacct cgccctgcaa tgggtcgccg
     6841 ttgaagcaac gacgctgttc tcggccccgc tcatctacct gcacggggac cgccggggga
     6901 tggaggccgc ctggaagtac ctgctcatct gctcggtcgg gatcgccttt gccttcctcg
     6961 ggatcatctt cctctcgcgg gcggccgggg acgtcccgct gacgtacacg gcgcttcccc
     7021 tcgcggcgag gcagctcgac ccacgcttcc tgcggctggc attcctcttc ttcctcgtgg
     7081 ggtacgggac caagatgggg ctcgccccga tgcacacatg gcttccggac gcgcacagcc
     7141 aggctcccgc gccggcgtcc gcgatgttgt cgggggtcct gctgaactgc gccctcctct
     7201 ccatcctgcg gatcgccccg gcgatccccg gcgcgtacgc ctctcccctg ctcatcctct
     7261 tcgggatcct gtcgatcgcg gtcccggcgc tcctcgtggc ggtccagcgg gacatcaaac
     7321 gtctgctcgc cttttcctcg atcgagaaca tggggatcgt cgtcctcggg ctcgggttcg
     7381 gcggcgccgc ccgaccggcc gcactgctgc acgtgctgaa ccactccgtg gccaaggtcc
     7441 tgctcttcct cgtcgccggg gacctcatcc tgcggttcgg cacgcagaag atccggcata
     7501 tccacgggat cctgcgggag tctccgctgc gcggcgtcct gctgttcggc gggatcctcg
     7561 cgatcacggg atccccgccg ttcggcgcct tctggagcga gttcggcatc ctcgtcgggg
     7621 ggatcgcggg cgggcacctg gccgtttccc tcgcgtacct cgtcctgctc gcgattcttt
     7681 tcggctcctt cctcatgtac ggcagcgaca tggtcttcgg ggaaggcgtg ccgcgaaaac
     7741 ttgccgcccc ggaactgtgg tgcctcctcc cggcgtgcgt cctccttctt gcgtccctct
     7801 ggctgggact ccacgtcccc gcgagcctgt acaagaccct ggagcggctc tccaccgtgg
     7861 tcgcgttctc ttgaccggcg aaaggaacaa gtcgatgcgc tgcgtccaca cgttcgacct
     7921 cggcctatcc gccccgctgg cggaggaagg tttccgcgcg ttcctcgggg aaacctccac
     7981 cggcggcgac cgcctcctcg cgctgttcgc ggaggagcct tccccgggac gatttcttct
     8041 tcacgcggtc gccgggggat ccgccccggg ccggttcgtt cacgcctgcg tggaggcttc
     8101 cccgcgatcc ttccgaacgt tcaccgacgt ctgccccgga acgcactggt tcgaacggga
     8161 gatctacgac ctcttcggcc tggtgccgga aggacacccc gagttgaacc ccctgcgctt
     8221 ccacgaaggg tggccgaaga agttcttccc gctgcggtcc gcggatgcgc ccacgcccga
     8281 tgcccgccgc ggctatcggt tccttctcgt acggggcgag gggatccacg aggtggcggt
     8341 gggacccgtc cacgccggga tcatcgaacc ggggcacttc cgtttttcct gcgccggaga
     8401 gacgatccag aacctcgaga tccgtctcgg ataccagcac aaggggatcg aatcgctctt
     8461 cccgggaggc ccggggatgg ggaagatcct cctttccgaa tcgatctccg gggacaccgc
     8521 cgtggggcac tcgaccgctt gcgcccgcct cctcgaagcg atcacgggga aacccgcgga
     8581 cccgtccgcg gagcgatggc gcgccgccgc gctcgagatc gagcggatct cgaaccattg
     8641 cggagacctc ggggggctct cgaccgacat cgggtacctg ttcggggcgt cccacttcgg
     8701 gcgcctgcgc ggcgcggcgc tgaatctgct gctgaaaatc acggggaacc gtttcgggcg
     8761 ggggctgaac cggatcgggg ggatcggccc ccgcttgccg gggatcgcgc cggaggacct
     8821 gatggcggaa ctcgggcgcc tgcgggccga agtggagaac gtcgggacga cgatgctgga
     8881 tgcctcgtcg gccgtcgccc ggatggaagg gacggggcgt ctcctcccgc gaaccgcgaa
     8941 gacgctccaa ctcgtcggcg ttgcggcccg cgcctccggc cgggacgtcg acgcgcggcg
     9001 ggacttcccc tctcctgcct acgtggaatc gcccccggcg aaggtgctgc tcgacaccgg
     9061 ggacgtgatg gcccgcgcaa gcgtccggtt catggagatg cgtgcctcgt tcgattggct
     9121 ttccggcatc ctcgccgcag cgatgccgcc gcgcacgatc gctgccgaag gggtcgtccc
     9181 cgcggcgggc cgccgcctgg tcgtctccgt cgaggagggg tggcggggcg agatcgtcca
     9241 tgcggccatc gtc
//
LOCUS       DASVJL010000264         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_40618, whole genome shotgun sequence.
ACCESSION   DASVJL010000264 DASVJL010000000
VERSION     DASVJL010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40618"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VF357_05530"
     CDS             <1..427
                     /locus_tag="VF357_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7523641.1"
                     /translation="AEEWVKEAVKEGAKVLIGGKREGRVMQPTVLVNVRSEMKVMCQE
                     AFAPLVSIYEYESFEDAVAMVEDSPYGLQAGIYTNDLKKAMYAVDRINVGGVMINDTS
                     IFRVDHMPYGGNKLSGLGREGVRFACEEMTSIKMVMIKP"
     gene            591..>1678
                     /locus_tag="VF357_05535"
     CDS             591..>1678
                     /locus_tag="VF357_05535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX7523642.1"
                     /translation="MPIELKLPDVGEGIAEGEVVRWLVAEGAQVKEDDLLVEILTDKA
                     NIELPSPVSGTFLKILAQPGQVVKVGEPIALIEPAAGQAVAAHHVTASSVRPISIPRE
                     AKEPGPESSSGKAAGADGAVLATPVVRKLAKDLGVELGEVPGSGPGGRITGEDVRRAA
                     GPKAPAGTPAEATADERIPFKGKRRMIARKMVAAKTRVPHALLVDEADVSGLLAERAK
                     MREIGEREGVRITILPFIMKAVAGALQRHPALNASLDEDREEIVLKKRVDVGMAVDTG
                     DGLVVPVVRNADAKSVIELAREIERLSAAAREGTLATADLTGGTFTISSVGSIGGLFT
                     YPIINVPEAAILAAHKIVTRPMVRDGEIV"
BASE COUNT          311 a          493 c          608 g          266 t
ORIGIN      
        1 ggcggaggag tgggtgaagg aggcggtcaa ggagggggcg aaggtgctga tcggcgggaa
       61 gcgcgagggg cgggtgatgc agccgacggt gctggtgaac gttcgctccg agatgaaggt
      121 gatgtgccag gaggcgttcg ccccgctggt ctccatttac gagtacgagt cgttcgagga
      181 cgcggtggcg atggtcgagg attccccgta cgggctgcag gcggggatct acaccaacga
      241 cctcaagaag gcgatgtacg ccgtggaccg gatcaacgtc ggcggggtga tgatcaacga
      301 cacctccatc ttccgcgtgg accacatgcc ctacggcggc aacaagttga gcggcctggg
      361 ccgcgaaggc gtccgcttcg cctgcgaaga gatgacgagc atcaagatgg tgatgatcaa
      421 gccgtaagtc cgccgcgggt ctcttcacgc aggactgctt cccatggcgc ccaagggtcc
      481 cgggtgggac ctttgggcgc ccttttttat gggataattg caatgccatc cagaattcgg
      541 aatatcattt ccggtagacg cgatcgcgtc cgaaaggggc ttcgcagacc atgccgatcg
      601 aactgaagct ccccgacgtg ggggagggga ttgcggaagg ggaagtcgtc cgctggctgg
      661 tggcggaagg tgcccaggtc aaggaggacg acctgctggt cgagatcctg accgacaagg
      721 cgaacatcga gcttccctcg ccggtgagcg ggaccttcct gaagatcctt gcgcagcccg
      781 gacaggtcgt gaaagtgggg gagcctatcg ccctcattga acctgcggca gggcaggccg
      841 tcgcagcgca tcacgtcacg gcatcgtccg ttcgtccgat ctcgatcccc cgggaagcga
      901 aagaaccggg accggaatct tcttccggga aagccgcagg cgcggacgga gcggtgctcg
      961 ccactcccgt cgttcggaaa ctggcgaagg acctcggggt ggagctgggt gaggtccccg
     1021 ggagcggtcc cggaggccgg atcaccggcg aggacgtgcg gcgagcggcg gggccgaagg
     1081 cgccggcggg cacccccgcg gaggccacgg cggacgagcg gatccccttc aaggggaaac
     1141 gccggatgat cgcgcggaag atggtggccg ccaagacccg tgttccccac gcattgctgg
     1201 tggacgaggc cgacgtctcg ggtctccttg ccgaacgcgc gaagatgcgg gagatcggcg
     1261 aacgcgaggg ggtgcggatc accattcttc ccttcatcat gaaggcggtc gccggggcgc
     1321 tccagcggca ccccgccctc aacgcctctc tcgacgagga ccgggaggag atcgtcctca
     1381 agaagagggt cgacgtcggc atggccgtgg ataccgggga tggattggtc gtcccggtcg
     1441 tccgcaacgc cgacgcgaaa tcggtcatcg aactggccag ggagatcgaa cggttgtcgg
     1501 cggcggcacg ggaggggacc ctcgccaccg cggatctgac cggcggcacc ttcacgatct
     1561 ccagcgtcgg ttcgatcggg gggctgttca cctatcccat tatcaacgtc ccggaggcgg
     1621 ccatcctggc ggcgcacaag atcgtgaccc ggcccatggt ccgcgacggg gagatcgt
//
LOCUS       DASVJL010000265         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_511353, whole genome shotgun sequence.
ACCESSION   DASVJL010000265 DASVJL010000000
VERSION     DASVJL010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511353"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /gene="purD"
                     /locus_tag="VF357_05540"
     CDS             complement(<1..1203)
                     /gene="purD"
                     /locus_tag="VF357_05540"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX7523643.1"
                     /translation="MNVLVIGSGGREHALVWKLAQSPRVERIYCTPGNPGIAGLALPF
                     PAEGGDFSLLIGEARRHEIGLTVVGPEGPLAAGIVDAFREAGLVIFGPTRRAAELEWS
                     KAFAKDFMKRRAIPGASYRTFHPGEEAGARSYILSCPLPLVIKADGLAAGKGVIVCRS
                     RDEAVRALGEISDPSSFGNAGSTVVVEEFMEGEEASVFAVTDGDRYVTLAPAQDHKRA
                     FDGDTGKNTGGMGAYAPAPCVTPSLLRRIEETIIVPTLRGMAEEGRPYSGCLYVGLML
                     SAEGPRVVEYNCRFGDPETQVVLPLYEGDLAELLVVSASGNVRAPSTPVPAVGSAVCV
                     VLASGGYPGAYETGKTITAPGTDETPPGVILFHAGTGRGPGGSLVTAGGRVIGVTAVR
                     RDGGMAETI"
     gene            complement(1205..>1612)
                     /locus_tag="VF357_05545"
     CDS             complement(1205..>1612)
                     /locus_tag="VF357_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523644.1"
                     /translation="ENLKLVAKLGLQDTVRVLGYLPHAECVRELCASDVLWMTVGDDL
                     GSPGKVYEYIGARKPILGLVPEGYLKSTIVEAGGKTVAPEDVGGIKNALGEFLLLHEK
                     HQLKGPDPGVVEKYDRRTLTGALAKIFESLFEP"
BASE COUNT          276 a          595 c          465 g          276 t
ORIGIN      
        1 gattgtctcc gccatcccgc cgtcccgccg caccgccgtt acgccgatca cgcgaccccc
       61 cgcggtgacg agtgaaccgc ccggcccccg tcccgtcccg gcgtggaaca ggatcacgcc
      121 ggggggagtt tcatccgtgc cgggcgcagt aatcgttttg ccggtctcgt acgcaccggg
      181 gtagccgccc gaggcaagga cgacgcacac cgcagatccc actgccggga ccggcgttga
      241 gggggccctg acattcccgg aagccgagac caccaggagt tccgccaggt ccccctcata
      301 gagcgggaga acgacctgcg tctcggggtc gccgaacctg cagttgtatt ccacgaccct
      361 gggtccctct gcactcagca tcagccccac gtagaggcag cccgaatagg gcctcccctc
      421 ttccgccatc cccctgagcg ttggaacgat gattgtttcc tcgatccgcc tcagaagcga
      481 aggggtcacg cacggggccg gggcatacgc ccccatcccc cccgtgttct tccccgtgtc
      541 tccgtcgaag gcccgtttgt gatcctgtgc aggggccagc gtgacatacc ggtcgccatc
      601 ggtcaccgca aataccgagg cctcctcccc ctccatgaat tcctcgacga cgacggtgct
      661 tccggcgttc ccgaacgagg agggatcgct tatctccccc agcgcccgca cagcctcatc
      721 gcgcgacctg cagacgatga cccctttccc cgctgccagc ccgtccgcct tgatcacgag
      781 cgggagggga cacgacagga tgtagctccg cgccccggcc tcttcccccg gatggaacgt
      841 cctgtaggag gcgcccggga ttgcgcggcg cttcatgaag tccttcgcga acgccttgct
      901 ccactccagt tcagccgcgc gccgcgtcgg accgaatatg acgagccccg cctccctgaa
      961 tgcgtccacg attcccgcgg caaggggccc ctcggggccg acgaccgtca ggccgatctc
     1021 atgccgccgg gcttctccga tcagcaggga aaaatctccg ccctcagccg gaaacggaag
     1081 ggcgagtccg gcgatccccg gattgcccgg cgtacagtag atcctctcca cgcgcgggct
     1141 ctgggcgagc ttccagacca gcgcgtgctc gcgtccgccg gaaccgatga cgagcacgtt
     1201 catgtcaggg ctcaaaaagt gactcaaaga tctttgccag cgccccggtg agcgtccggc
     1261 ggtcgtactt ttccacaacc ccggggtccg ggcccttcaa ctggtgcttc tcgtggagca
     1321 gaaggaattc cccgagagca ttcttgattc cgccgacatc ctccggggcg acggtcttcc
     1381 cccccgcttc aactatcgtg gatttcagat acccttccgg caccaggccg aggatcggct
     1441 tgcgcgcccc gatatattcg tacaccttcc ccggcgaccc caggtcgtcc cccaccgtca
     1501 tccagaggac gtccgaagcg cagagctcgc ggacgcattc ggcgtgcgga agataaccga
     1561 ggacacgcac ggtatcctgg agcccgagct tcgcgacgag tttcaaattc tc
//
LOCUS       DASVJL010000266         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1074334, whole genome shotgun sequence.
ACCESSION   DASVJL010000266 DASVJL010000000
VERSION     DASVJL010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074334"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VF357_05550"
     CDS             <1..824
                     /locus_tag="VF357_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7523645.1"
                     /translation="GPGVSATQIVFYLLYNSLVPATRTRNKLADLGAVAAGLAHEIRN
                     PLNSLYINGQLLEEMLSELPEEGVPQRVDLLSLARANLKVTQRLNDTLSGFLRFARPP
                     AMELALVDLNRIVSETLRFLEMELAYRGVSLKTKLHPTPLPILADEKMLKQALLNLLL
                     NAQEAMDKEEKEIRVATGVQAGRPFVRIHDNGRGIPASDRRHIFRLFFTTRKNGSGLG
                     LPIVRQIVRQHGGAVYVRSREGQGTTMTVALLFEEQFRAMFPGRLPLALPERGRT"
     gene            821..2191
                     /locus_tag="VF357_05555"
     CDS             821..2191
                     /locus_tag="VF357_05555"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7523646.1"
                     /translation="MNQREKGRVFVFDDDADSLQSVVAALRRDGFDVFPFADPREGLA
                     RIEAEGGDVVVTDLRMPGLTGLEVLRHVTKKIPEVPVVILTAYGTVEGAIEAVRAGAS
                     DFLLKPVEIPRLRAAVFKALKERGMHREIERLREETGRPLGIDGIVGSSRAMEAVLRK
                     IRLVAPTRMNVLITGESGTGKELVARGVHGLSPRADRPFLPLNCAAIPETLLESELFG
                     HEKGAFTGATASRPGKFENAEGGTLFLDEVGDVSPAIQSKLLRAIEQKEVMRVGGSLV
                     IYADVRIIAATNQDLKGRVEAKAFREDLYYRLNVFNIVVPPLRERREDIPKLCDHFLA
                     LIGKENGLPAKRLSPAALKSLLACRWPGNVRQLRNALDTATLVSQGDTIEPGDLPPEV
                     TQAVLPPSSSEPIPLPASRTLTELERDAIRAALVETAGNKTLAAKLLGIGLRTLHRKV
                     KEFGID"
BASE COUNT          413 a          796 c          772 g          375 t
ORIGIN      
        1 ggggaccggg cgtttccgca acgcaaatcg tattttatct gttgtacaat tcactcgtgc
       61 cggccacgag aacccgtaat aagctcgcgg acctgggcgc cgtcgcggcg ggcctggccc
      121 acgagatccg caacccgctg aactccctct acatcaacgg ccagctcctc gaggagatgc
      181 tctccgagct gccggaggag ggcgttccgc agagggtgga cctcctctcc ctcgcccgcg
      241 ccaacctcaa ggtcacgcag cggctgaacg acacgctctc gggcttcctg cgcttcgccc
      301 ggcccccggc gatggaactt gccctggtcg acctcaacag gatcgtgtcg gagacgctgc
      361 ggttcctcga gatggaactc gcgtaccggg gggtctccct caagacgaag ctccacccca
      421 cacccctgcc gatcctggcc gacgagaaaa tgttgaagca ggcgcttctc aacctccttc
      481 tgaacgcgca ggaggcgatg gacaaggagg agaaggagat ccgcgtggcg accggcgtgc
      541 aggcggggcg cccgttcgtg cggatccatg acaacggccg ggggatcccg gcttccgacc
      601 gccgccacat cttccgcctc ttcttcacca cccggaagaa cggcagcggg ttggggcttc
      661 cgatcgtccg ccagatcgtg cggcagcacg gcggggcggt ctacgtccgc agccgggagg
      721 ggcaagggac cacgatgacc gtcgcgctcc tgttcgagga gcagttccgg gcgatgttcc
      781 ccggccgcct gccgctggcg ctgcctgaga gggggcgtac atgaaccaga gggagaaggg
      841 gcgtgtcttc gtcttcgacg acgacgccga cagcctccag agcgtcgtcg ccgcgctgcg
      901 ccgcgacggt ttcgacgtgt tccccttcgc cgacccgcgg gaaggattgg cgcgcatcga
      961 agccgagggg ggtgacgtcg tcgtcaccga cctgaggatg ccgggcctga ccggcctcga
     1021 ggtgctccgc cacgtcacga agaagatccc ggaggtcccg gtggtcatcc tgacggcgta
     1081 cggcaccgtc gaaggggcga tcgaggcggt gcgcgccggc gcctcggatt tcctcctcaa
     1141 gcccgtggag attccccgtc tgcgggccgc cgtcttcaag gcgctcaagg agcgcgggat
     1201 gcaccgggag atcgagcggc tccgcgagga gaccggccgg ccgctcggga tcgacgggat
     1261 cgtcggatcc tctcgcgcga tggaagcggt cctgcggaag atccgtctgg tcgctccgac
     1321 ccggatgaac gtcctgatca ccggggaaag cggcacgggg aaggaactcg tggcgcgggg
     1381 ggtccacggc ctgagccccc gcgccgaccg gcccttcctg ccgttgaact gcgccgcgat
     1441 cccggagacg ctcctcgagt ccgagctctt cggccacgag aagggggcgt tcacgggggc
     1501 gaccgcctcc cggccgggga agttcgagaa cgccgagggg gggacgctct tcctcgacga
     1561 ggtcggggac gtgtccccgg cgatccagtc gaagctcctg cgcgccatcg agcagaagga
     1621 ggtcatgcgg gtcggcggat cgctggtaat ctacgcggac gtgcggatca tcgcggcgac
     1681 gaaccaggac ctgaaaggac gggtggaggc gaaggcgttc cgcgaagatc tctactaccg
     1741 gctgaacgtg ttcaacatcg tcgtcccgcc gctgcgggaa cgccgcgagg acatcccgaa
     1801 actgtgcgac cacttccttg cgctgatcgg gaaagagaac gggcttcccg cgaagcggct
     1861 ttcccccgcg gcgctgaaaa gcctgctcgc ctgccgatgg cccgggaacg tccgccagct
     1921 gcgcaacgcc ctggataccg ccaccctggt ttcccagggg gacacgatcg aacccggaga
     1981 cctccccccc gaggtgacgc aggcggtcct cccgccttcc tcctcggagc cgatccccct
     2041 tccggcatcc aggactctca cggaactgga gcgggacgcc atccgagccg ccctcgtcga
     2101 gaccgcgggg aacaagaccc tggcggcgaa gcttctcggg atcggcctgc gcaccctcca
     2161 ccgcaaggtc aaggagttcg ggatcgactg aacgccggcg ccaacacgcc atcagcaagc
     2221 acagatatac gccataatgg cacggcgcgc cttccgatct gtgccttaat ggcgcacatc
     2281 ctccgtgccc tcccttcggc aaaggatcct tcctaatctt tttatattat aaaaacagat
     2341 agttgttgat atattg
//
LOCUS       DASVJL010000267         1743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_417496, whole genome shotgun sequence.
ACCESSION   DASVJL010000267 DASVJL010000000
VERSION     DASVJL010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417496"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VF357_05560"
     CDS             complement(<1..746)
                     /locus_tag="VF357_05560"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX7523647.1"
                     /translation="MSRLGIAVLGATGSVGRNALDVISRFPRRFRATALCAGTDARSL
                     AGLARLFRPDTVCLSGGNGADLRKEFPRGTRLLFGEEGMREAACAGNVDIVLAAASGI
                     SSIRPVIAAAEQGKRIALANKELLVMAGKFVTAAARRGKAEILPVDSEHSAVFQAIAG
                     RRRDEILRILLTASGGPFRTYTVARMRSATVAQALGHPTWRMGAKISVDSATLMNKGL
                     EVIEATWLFGLPPSRIDVVIHPQSVVHSMV"
     gene            complement(743..1564)
                     /locus_tag="VF357_05565"
     CDS             complement(743..1564)
                     /locus_tag="VF357_05565"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX7523648.1"
                     /translation="MLWKRIATGAVLVTLLIASILFSVGRPWGWPFLLFCAVSAALCA
                     AEGFRMFLHGARDRAGGVALAVLVYFSAALLPGSFGVPAVLVCVLLSVFHVLPGAADP
                     AEKARRAATLCLGAVYVGGLLSTYPRTLLLPRGEHWVLLGILAVAAGDTMAYFTGRAI
                     GRRMLAPVVSPNKTVEGAVGGLLGSIGCAVLYAHGFLPGVPAGYAAAAGAAVGIFGQV
                     GDLFESLIKRAAGVKNSGTLLPGHGGILDRADGILAAGPVLYLFAALASRAGVGA"
BASE COUNT          311 a          628 c          591 g          213 t
ORIGIN      
        1 tccaccatcg agtggaccac cgattggggg tggatcacca cgtcgatccg cgaggggggg
       61 aggccgaaca gccacgtcgc ctcgatcacc tcgagccctt tgttcatgag ggtcgcggag
      121 tccacggaga tcttcgcccc catccgccac gtggggtgtc ccagcgcctg cgcgacggta
      181 gccgaccgca tccgtgcgac ggtgtaagtg cggaacgggc cgccggaggc ggtaaggagg
      241 atccggagga tctcgtcccg gcggcggccc gcgatcgcct ggaagacggc ggaatgctca
      301 ctgtcgacgg ggaggatctc cgccttcccc cgtcgcgcag cggcggtcac gaacttcccg
      361 gccatcacga gaagctcctt gttcgccagg gcgatccgct tcccctgttc cgcggcggcg
      421 atgaccggac ggatcgagga gatcccggac gccgcggcga ggacgatgtc gacgttcccc
      481 gcgcacgccg cctcccgcat cccctcttcc ccgaagagga ggcgcgttcc ccttggaaac
      541 tccttccgga gatccgcccc gttccccccg gagagacaga ccgtgtcggg gcggaaaaga
      601 cgggcgagac cggccaggga ccgggcgtcc gtccccgcgc acagcgcggt cgcgcgaaac
      661 cggcgcggga accgggagat gacgtccagc gcgttccggc cgacggagcc ggtggcgccc
      721 agcaccgcta tccccaggcg gctcacgcgc ccaccccggc gcgggacgcc aacgccgcga
      781 agaggtaaag gaccgggccg gccgcgagga tcccgtcggc ccgatcgagg atccccccgt
      841 gaccaggcag tagcgtcccg ctgttcttca cgcccgccgc gcgcttgatc agcgactcga
      901 acaggtcccc cacctgtccg aagatcccca ccgcggcccc ggcggcggcg gcgtatcccg
      961 ccggcacccc ggggagaaaa ccgtgcgcgt agaggacggc gcagccgatg ctcccgagga
     1021 ggcccccgac cgccccttcc accgtcttgt tcggcgagac gaccggggcg agcatcctcc
     1081 ggccgatcgc ccttcccgtg aagtaggcca tcgtatcccc cgccgccacg gcgagaatcc
     1141 ccaggagcac ccagtgctcc ccgcggggaa ggaggagcgt ccgcggatac gtcgacagga
     1201 gtcccccgac gtagacagcc ccgaggcaga gcgtcgccgc cctgcgcgct ttttccgcgg
     1261 ggtcggccgc tcccggaagg acgtggaaga ccgacaggag gacgcagacg agaactgccg
     1321 ggacaccgaa ggaacccggg agcagcgccg ccgaaaaata cacgagtacg gcgagcgcga
     1381 ccccgcccgc gcgatccctg gcgccgtgga ggaacatccg gaatccttcg gccgcgcaaa
     1441 gcgccgcgga aacggcgcag aacaacagga acggccaccc ccacggccgc ccgacggaga
     1501 acaggatgga tgcgatcaag agcgtgacga gaacagcacc ggtggcgatc cgtttccaga
     1561 gcatcgctac ttccccgggg gcttggcggc ttgctcgccg gtcagcccga agcgcctgtg
     1621 gcgacgggag tactcctcga tcgcctggag gaactccgcc ttgccgaaat ccggccacag
     1681 cacgtccgtg aaggcgaact cggcgtaggc ggattgccag agcaggaagt tgctgatgcg
     1741 gag
//
LOCUS       DASVJL010000268         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1074828, whole genome shotgun sequence.
ACCESSION   DASVJL010000268 DASVJL010000000
VERSION     DASVJL010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074828"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="VF357_05570"
     CDS             <1..120
                     /locus_tag="VF357_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015506105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX7523649.1"
                     /translation="EEVGQDFEVTRERIRQIEAKALRKLRHPSRSKRLRTFME"
     gene            196..272
                     /locus_tag="VF357_05575"
     tRNA            196..272
                     /locus_tag="VF357_05575"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:230..232,aa:Ile,seq:cat)
     gene            382..1536
                     /locus_tag="VF357_05580"
     CDS             382..1536
                     /locus_tag="VF357_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX7523650.1"
                     /translation="MRRRAAVTVNDVLRALDERFPFAHCADWDNVGILLGDPDAPVRS
                     VLIALDATPTVIARLRRRPADLLVTHHPVVFTPLKSVRPDPGPSAAAFALLRMGVAVI
                     SAHTNADAAPRGVSHAMGRRLRLRGMRPLIPGEPSSGACKIVAFVPPSHAEAVLAAAV
                     EAGGARIGAYSRCSFRTPGTGTFLGGPDSSPRFGTPGSEERVEEVRLETVAPGSRVRA
                     VMCAVRKAHPYEEPAIDVVPLREGALGGGIGIVGELPEPLPLDRALDAVRLALRPSWI
                     HVAGPRRKTVRRVAIVGGSGAEFSFAAREAGADLYVTGDVKYHQALDAAAGDMPVADI
                     GHASGERWILPEFRRVISARFSGAVSARVIMDEEPLRAVPAGRRRGGEKP"
     gene            1536..2261
                     /locus_tag="VF357_05585"
     CDS             1536..2261
                     /locus_tag="VF357_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX7523651.1"
                     /translation="MEQVKILIDLQEVMSQARRLDEEKQRIPLEVADLKSLFEEREAS
                     FLAAEQEFETLKQQRREKEREIEEERDKVERAKSKLMSIKTNKEYYAMLKEIEGTRRT
                     NVAREEELLSLLVRYEEAEKRLAERKADLDEVSGRYRERMVDIDARMGTFDVDIGKLD
                     ARRRHVSSKLDPSLVRRFEMIFERRDGLAVVAARNYSCMGCHMNIAPQLFNLLQREDR
                     IHTCPNCNRVVYFEATEIEAAGE"
     gene            2258..2674
                     /locus_tag="VF357_05590"
     CDS             2258..2674
                     /locus_tag="VF357_05590"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX7523652.1"
                     /translation="MTPVDVTVRVDGASRGNPGPSGAGAVVEFGDGRAPRELCSYLGE
                     TTNNVAEYRALLLALEEVVRHAVSSLTVHSDSELLVRQLKGEYKVKAEHLRPLHAEAC
                     RRLRAFPMVRILHVPREENRRADSLANLAIDQHRKG"
     gene            2681..3094
                     /gene="rnpB"
                     /locus_tag="VF357_05595"
     ncRNA           2681..3094
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF357_05595"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3266..>3642
                     /gene="mraZ"
                     /locus_tag="VF357_05600"
     CDS             3266..>3642
                     /gene="mraZ"
                     /locus_tag="VF357_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX7523653.1"
                     /translation="MIFRGRFEYTIDPKGRVNIPAPFRDRLQESAQGSFFLTNFSDCL
                     YVFAADDWARIEERLSRVPSTDRKMNAFVRFFLGGAVEVVPDKQGRILVPPSLRSYAG
                     LEKEVVILGMPNRFEIWSLARWQ"
BASE COUNT          628 a         1139 c         1290 g          585 t
ORIGIN      
        1 gaggaggtgg gccaggactt cgaggtgacc cgggagcgga tccgccagat cgaggcgaag
       61 gcgctccgga agctgcgcca ccccagccgg agcaagcggc tgcggacctt catggagtag
      121 tgaaggtgcg gacggcattg acacctcgaa agcgggcgga agataatgaa aagatgacct
      181 ggagttcctc gggttgggcc catagctcag ttggtcagag ccaccggctc ataaccggtt
      241 ggtccctggt tcgaggccag gtgggcccac caatatcgac gttgccatgg ggcctggcgt
      301 gagcgggtcc gcgatggaca cggcacgaaa tgcaccggct atcggtgcat ttttatttaa
      361 ggcctgcggc aaggtgcctg cgtgagacgc cgtgccgcgg tcacggtgaa cgacgtgttg
      421 cgggcgctcg atgagcgttt cccgttcgcc cactgcgccg actgggacaa tgtagggatc
      481 ctgctgggcg accccgacgc tcccgtgcgg tcggtcctca tcgcgctcga cgccacgccg
      541 acggtgatcg cgcgccttcg acgacgcccc gccgatctcc tggtcacgca ccaccccgtc
      601 gtgttcaccc ccctgaaatc cgtccgtccg gatcccgggc cttccgccgc ggcgttcgcc
      661 ctgctgcgga tgggcgtggc ggtcatctcc gctcacacca acgccgacgc ggctccgcgg
      721 ggcgtttcgc acgcgatggg ccgccgcctg cggctgcgcg ggatgcgccc gctgatcccc
      781 ggcgaaccgt cctccggcgc gtgcaaaatc gtggcgttcg ttcctccgtc ccacgccgaa
      841 gccgtgctgg ccgcagccgt ggaggcgggc ggggcgcgga tcggcgcgta ttcccgctgt
      901 tccttccgga ccccggggac cggcacgttc ctcggcggac cggattcctc tccgcgcttt
      961 ggaacaccgg gatcggagga gcgcgtggag gaggtccggc tcgagacggt cgcgccgggg
     1021 agccgggtgc gggcggtgat gtgcgcggtg cgaaaggcgc acccgtacga ggagccggcg
     1081 atcgacgtcg tgccgttgcg cgagggggcc ctcggaggcg ggatcgggat cgtcggcgag
     1141 ttgcccgaac cgctgccgct cgaccgggct ctcgacgcgg tgcgccttgc cctgcggccc
     1201 tcctggatcc acgtcgcggg tccccgccgg aagacggtgc gccgtgtcgc gatcgtcggc
     1261 gggagcgggg cggagttctc cttcgctgcg cgcgaggccg gagcggacct ctacgtcacg
     1321 ggggatgtga agtaccacca ggcgctggat gcggcggcgg gagacatgcc cgtcgccgac
     1381 atcgggcatg cttcggggga acgatggatc ctgccggaat tccggcgggt catcagcgcg
     1441 cggttttcgg gggccgtgtc ggcccgtgtc atcatggacg aagagccttt gcgggctgtg
     1501 cccgcagggc gaagaagggg aggggaaaag ccttgatgga gcaagtgaag atcctgatcg
     1561 atctgcagga ggtaatgagt caggcacggc ggctcgacga ggagaagcag aggatccccc
     1621 tcgaggtggc ggatctgaag agcctcttcg aagagcgcga ggcgtcgttt ctggcggcgg
     1681 agcaggagtt cgagaccctc aagcagcagc gccgcgagaa ggagcgggag atcgaggagg
     1741 agcgggacaa ggtcgagcgc gcgaagtcga agctcatgtc gatcaagacg aacaaggaat
     1801 attacgccat gctcaaggag atcgagggaa cccggcggac gaacgtagcc cgcgaagagg
     1861 aacttctctc ccttctcgtg cgctacgagg aggcggaaaa gcggctcgcg gagcggaagg
     1921 ccgatctcga cgaggtgtcg gggagatacc gggagcggat ggtcgacatt gacgcccgga
     1981 tgggaacctt cgacgtcgac atcgggaagc tggacgcgcg caggcgccat gtgtcgagca
     2041 agctggatcc gtccctcgtg cgccgtttcg agatgatctt cgaacggcgg gacggcttgg
     2101 cggtcgtcgc ggcgaggaac tactcctgca tggggtgcca catgaacatc gccccgcagt
     2161 tgttcaacct tctgcagcgg gaggaccgga tccacacctg ccccaactgc aaccgtgtcg
     2221 tgtatttcga ggcgacggag atcgaggcgg ccggcgagtg acccccgtcg acgtcacggt
     2281 gcgcgtcgac ggcgccagcc gcgggaatcc cgggccctcc ggcgccggcg cggtggtcga
     2341 gttcggggac gggcgggcgc cgagggagct ttgcagctac ctcggggaga cgacgaacaa
     2401 cgtggcggaa taccgcgcgc tgctcctggc gctcgaagag gtggtccggc acgccgtctc
     2461 ctccctcacc gtccactccg attcggaact cctcgtccgg cagttgaagg gagagtacaa
     2521 ggtgaaggcg gagcacctcc ggccgctcca cgcggaggcg tgccgccggt tgcgcgcttt
     2581 cccgatggtc cgtatactgc atgtaccgcg cgaggagaac cggagagcgg attccctggc
     2641 gaacctcgcg atcgatcagc accggaaagg ataagtcgtc cgaggaggcc aggtggccgc
     2701 cggtccccgc gccgcatcgg cggggacggg aggaaagtcc gggctccgca gggcagggtg
     2761 ccgggtaaca cccggtgggg gcgaccccga ggacagcgcc acagaaaaga caccgcctcc
     2821 cgcttcggcg ggcggtaagg gtgaaatggt gaggtaagag ctcaccgcgc gacgggcgat
     2881 cgtcgcggca tggcaaaccc cacccggagc aagaccaaat aggagggcga ccccgaggcg
     2941 gctctgttgc cggcggggga agggcggccc gcccggggcc ctcgggtcaa ggtcgctcaa
     3001 ggcgggcgga aacgcccgcc gtagagaaat ggccgccccg cggcgacgcg tttcgtcgcc
     3061 gcgcacagaa cccggcttac ggcctcctcg gacactttct ccggcgcgcg gcactccccc
     3121 cgataaatta aattccgttt tcgattcttg cgtcccccga ggtttttttt cctcgaaggg
     3181 atcgaaatac cttgacaccc ccgaaggtct cgataaaata cccaacaagt gggtaattgt
     3241 ggggaaaggt gggtaacggg ccaccatgat cttccgggga cgcttcgaat ataccattga
     3301 tccaaaaggc cgggtcaaca tccccgcgcc cttccgcgac cgtctccagg aatccgccca
     3361 gggatcgttt ttcctcacca acttctcgga ctgcctctat gttttcgccg cggacgactg
     3421 ggcgcggatc gaggagcggc tttcccgtgt tcccagcacc gaccggaaga tgaacgcctt
     3481 tgtccggttt ttcctcggag gcgccgtcga ggtcgtcccc gacaagcagg gtaggatcct
     3541 cgttccgccc tccctgcgtt cctacgcggg gctggaaaag gaggtcgtga tcctcgggat
     3601 gcccaaccgg ttcgagatct ggtcgctcgc ccggtggcag ga
//
LOCUS       DASVJL010000269         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1355341, whole genome shotgun sequence.
ACCESSION   DASVJL010000269 DASVJL010000000
VERSION     DASVJL010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355341"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VF357_05605"
     CDS             <1..823
                     /locus_tag="VF357_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX7523654.1"
                     /translation="RVPLPRETAVLRWDPVKREVDAAGLEGFDAVIHLAGENVGAGRW
                     TAARKAAIRDSRVNGTRLLCETLAGLARPPKTLVCASAIGYYGDRGEEPLTEESPPGT
                     GFLAEVCREWEAASAPAAQKGIRVVTLRIGMVLSPKGGALARMLPLFRVGLGGVIGGG
                     RQYVSWVALDDLPHIIHHVLQRGDLSGPVNAVAPRPVTNREFTEALGKALSRPTPLPV
                     PAFALRLAVGGEMADALLLASGRGIPRRLEETGYRFRFPELPAALRHLLGKGGTP"
     gene            complement(957..>1797)
                     /locus_tag="VF357_05610"
     CDS             complement(957..>1797)
                     /locus_tag="VF357_05610"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7523655.1"
                     /translation="YNVTVYSRTKEKAHPLLVKGAKWADSPGAVAGSSDVVFSMVGFP
                     ADVREVYFGPNGVLACSRAGTIVVDMTTTEPSLAKEIYDAAKVRGDVHAVDAPVSGGD
                     VGAREARLSIMIGGDPEPVAALAPLFEAMGKTIVRQGGPGAGQHAKMCNQIVIAGTMI
                     GVCESLIYGHKAGLSPETMLSSISGGAAGCWALSNLAPRILKRNFDPGFFVEHFVKDM
                     GIALDEARRMNLPLPGLALVHQMYVALQANGGGKHGTQALMLVLEQMSNTKIEKGGAP
                     TPG"
BASE COUNT          271 a          613 c          616 g          297 t
ORIGIN      
        1 gcgcgtgccc ctcccaaggg aaacggcggt gctgcggtgg gacccggtga agcgggaggt
       61 cgacgcggcc gggctcgagg ggttcgacgc ggtgatccac ctggcgggcg agaacgtcgg
      121 cgccggccgg tggaccgccg cgcggaaagc cgccatccgc gacagccgcg tgaacgggac
      181 gcgcctcctc tgcgagactc tcgcggggct cgcccggccg ccgaagacgc tcgtgtgcgc
      241 ctccgcgatc ggctattacg gagaccgggg agaggaaccg ttgaccgagg agagtccgcc
      301 cggcacagga tttctcgccg aggtctgccg ggagtgggag gcggcctcag cgccggcggc
      361 acagaaggga atccgcgtcg tcaccttgcg gatcgggatg gtgctctcgc caaaaggggg
      421 ggcgctcgcg cggatgctcc cgctgttccg ggtggggctc gggggcgtga tcggaggcgg
      481 acgtcagtac gtcagctggg tcgccctcga cgacctcccc cacatcatcc accatgtatt
      541 gcagcgcggc gacctgagcg gtccggtcaa tgccgtcgca ccgcgcccgg tcaccaaccg
      601 ggagtttacc gaggcgctcg ggaaggcttt gtcgcgcccg acgccgcttc cagtcccggc
      661 cttcgccctc cgcctcgcgg tggggggcga gatggcggac gccctgctgc tggcgagcgg
      721 gcgcgggatc ccgcgccgcc tcgaggagac cggctaccga ttccgctttc cggagcttcc
      781 cgccgcgttg cgccacctgc tcggaaaagg ggggacacca tgagggtgtt gctgttcggc
      841 acgtgcctcg tggatacctt catcccgatg cccaacgccg cccgccgata tcgagctcac
      901 ccgggcgatc ggcgtgcacg gccccgagag gctcgacatc ctcgtcgcgt agcgtctcac
      961 cccggggtcg gagcgccgcc cttttcgatt ttcgtgttgc tcatctgttc cagtacgagc
     1021 atcagcgcct gggtgccgtg tttcccgccc ccgttggcct gcagcgccac gtacatctgg
     1081 tggaccagcg cgaggccggg caacgggagg ttcatcctcc gtgcctcgtc gagggcgatc
     1141 cccatatcct tgacgaaatg ctcgacgaag aagcccggat cgaaattgcg cttgaggatc
     1201 ctcggggcga ggttcgacag cgcccagcac ccggcggcgc cgccactgat ggaggagagc
     1261 atcgtttcgg gggaaagccc cgccttgtgc ccgtagatca gcgactcgca aacgccgatc
     1321 atggtcccgg cgatcacgat ctggttgcac atcttcgcgt gctgaccggc tccggggccg
     1381 ccttggcgga cgatcgtctt tcccatcgcc tcgaacaggg gagcaagggc ggcgacgggt
     1441 tcggggtcgc ctccgatcat gatggaaagg cgcgcctcgc gggctcccac atcaccgccg
     1501 gagacggggg cgtccacggc gtgtacgtct ccccggacct tcgccgcatc gtagatctcc
     1561 ttcgccagcg acggttccgt ggtggtcatg tccacgacga tggtcccggc cctggagcag
     1621 gccaacactc cgttcggtcc gaagtagacc tcccgcacat ccgcgggaaa gccgaccatg
     1681 ctgaacacca cgtcggaaga tcccgccacg gcgccaggag agtccgccca ttttgctccc
     1741 ttcacgagga gggggtgcgc cttttccttc gtccgggagt acacggtgac gttgtac
//
LOCUS       DASVJL010000270         3813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_137630, whole genome shotgun sequence.
ACCESSION   DASVJL010000270 DASVJL010000000
VERSION     DASVJL010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137630"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..1037)
                     /gene="lgt"
                     /locus_tag="VF357_05615"
     CDS             complement(270..1037)
                     /gene="lgt"
                     /locus_tag="VF357_05615"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX7523656.1"
                     /translation="MHPVLLQIGSLKIYSYGVFVAIGFLAALWVSGREIARQGLDREK
                     FFDMGFWVVLSAIAGARIFHVLVYWRQYAEAPGEILKLWNGGLVFYGGFLAATAACVV
                     FLRRNRMPFLPVADASSLGIVLGLAFGRLGCTSAGCCFGKPTTLPWAITFTDPACLAP
                     LHVPLHPTQIYESIGGFAIFGFLYITRDRFKTPGMRFWTMLILYGAARAFFEIFRDDP
                     RGFLGPFSESQVVSAVLITYAVVSILRARAKATPATR"
     gene            complement(1009..1530)
                     /gene="lspA"
                     /locus_tag="VF357_05620"
     CDS             complement(1009..1530)
                     /gene="lspA"
                     /locus_tag="VF357_05620"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX7523657.1"
                     /translation="MPVTFLRKFSVPVVSALLLGAVDQATKIWAVRNLPMYEPLELVH
                     GFFDLVHVRNTGVAFSLLSNLDHRWVHPFLILATVLAMGAVLAYIAYLPCRGAAPVGL
                     GLILGGAIGNLIDRARLGYVVDFIDLYWRGHHWPTFNVADVGITVGVVLLVIDMVVSP
                     KEPGDASRPAADR"
     gene            complement(1535..>3813)
                     /gene="ileS"
                     /locus_tag="VF357_05625"
     CDS             complement(1535..>3813)
                     /gene="ileS"
                     /locus_tag="VF357_05625"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX7523658.1"
                     /translation="RFVESGAVYQGTKPVYWCLSCRTALAEAEVEYADHTSPSIHVKF
                     PFVEPPERIHPALAGRKVFFVIWTTTPWTIPANLGIALHPDYDYVALEAGGEVYVVAE
                     GLAERFAAETGLSSPTILATFRAGRLERMRCRHPFADRDSLLVLADYVTLDAGTGCVH
                     TAPGHGREDYETGLKYGLPILAPLNDEGRFTADVPFFAGERVFDANPKVNEKLAEVGA
                     LMARREITHSYPHCWRCKSPVIFRATKQWFISMDGTRLREKALAEIRKVRWIPSWGQE
                     RIEGMIANRPDWCISRQRAWGVPIALFRCEGCGHHLLDRKLVDHVAGFFEKEGADAWF
                     DRGVSELLPPGTACPECGGTAFGKETDILDVWFDSGVSYACVCEGKENLGIPVDLYLE
                     GSDQHRGWFHSSLLAAVGTRGIPPYRGVLTHGFVVDGKGEAMHKSKGNVIAPEGIIKK
                     HGVELLRLWVAAEDYRDDIRLSKDILDRLTEAYRKIRNTIRYLLASLSDFDPARDAVP
                     VDRMEEMDRFALVLFDRLASKVRNAYEEYEFHVLFHAVNNFCSVDMSSFYLNVLKDRM
                     YCSPAVDPARRSAQTAIFEIARGLLSLAAPVLSFTTEEAWGYLPAYPGKPESVFLSDL
                     PKPLRIPDAEAIAARWQRILELRSEVGQPLETARREKVIGSGQDALVTIAPGPFADLF
                     ETHAREIRDALIVSGIVVGEVSGPGMYESAAFPGLKVAVEKAPWGKCERCWNHAPEVG
                     TDAGAPELCRRCAAAVGK"
BASE COUNT          647 a         1297 c         1318 g          551 t
ORIGIN      
        1 gtcgtcggcg gcgccctgcc gtagttctcc ccttcattgc agacggtgcc cgtcgatgaa
       61 cacgcgcatg cctggcgggg cactgacgca ggaggccttc ccctgaacgg gcgtctgagg
      121 cacccggagc catggagggc gggaggatgc cgaggcgcag cgaggacggc cagggacggc
      181 cgtcgagcgg tagtgaaggg gaagggcctc cgaggaagag gggccccgca gggcccacta
      241 agaggcgaac gatcgggcgg aggggccggc taccgggttg cgggggttgc cttcgcccgg
      301 gcgcggagaa tggagacgac ggcgtaagtg atcaggacgg ccgagacgac ctgcgactcg
      361 gagaaggggc cgaggaagcc gcgggggtcg tcgcggaaga tctcgaagaa cgcccgcgcc
      421 gcgccgtaca ggatcagcat cgtccagaag cgcatcccgg gcgtcttgaa ccggtcccgc
      481 gtgatgtaga ggaagccgaa gatggcgaac cccccgatcg attcgtagat ctgcgtcggg
      541 tggagcggca cgtggagcgg cgcgaggcag gcggggtcgg tgaaggtgat cgcccagggg
      601 agggtggtcg gcttcccgaa gcagcatccg gccgaggtgc agccgagccg cccgaacgcg
      661 agcccgagca cgatcccgag cgacgacgcg tcggccaccg gcaggaacgg catccggttc
      721 ctccggagga acacgacgca ggccgccgtc gcggcgagga acccgccgta gaagaccagc
      781 ccgccgttcc acaatttgag tatctccccg ggcgcctccg cgtattgccg ccagtagacg
      841 aggacgtgga agatcctcgc ccccgcgatg gcggagagga ccacccagaa ccccatgtcg
      901 aagaacttct cgcggtcgag cccctggcgg gcgatctccc ggccggagac ccacagggcg
      961 gcgaggaagc cgatcgcgac gaagacgccg tacgagtaga tcttgaggct accgatctgc
     1021 agcaggacgg gatgcatccc cgggctcctt cggcgagacc accatgtcga tcacgagcag
     1081 gacgacgccc acggtgatcc cgacgtccgc aacgttgaag gtgggccagt ggtggccgcg
     1141 ccagtacagg tcgatgaaat cgacgacgta ccccagccgc gcccggtcga tcaggttccc
     1201 gatcgctccg ccgaggatga gccccaggcc caccggcgcc gcccccctgc aggggaggta
     1261 cgcgatgtag gcgagcaccg cacccatcgc gagcaccgtc gcgaggatca ggaacgggtg
     1321 gacccagcgg tgatcgaggt tcgaaagaag gctgaaggcg acccccgtgt tgcgcacgtg
     1381 caccaggtcg aagaagccgt ggacgagttc gaggggctcg tacatgggga ggttgcgcac
     1441 cgcccagatc ttggtcgcct ggtcgacggc gccgagcagg agcgccgaga cgaccggcac
     1501 cgaaaatttc cgcaggaagg ttaccggcaa ggcgtcactt ccccacggcg gcggcgcacc
     1561 gccggcacag ctccggcgcc ccggcgtccg ttcccacctc gggcgcgtgg ttccagcacc
     1621 gttcgcattt tccccagggc gccttttcca ccgcgacctt cagccccggg aaggcggcgc
     1681 tctcgtacat cccgggcccg gacacctcgc ccacgacgat ccccgagacg atcaacgcgt
     1741 cgcggatctc ccgggcgtgc gtttcgaaga ggtccgcgaa ggggcccggg gcgatcgtta
     1801 ccagcgcgtc ctggccgctg ccgatcacct tttccctgcg cgccgtctcc agcggctgcc
     1861 cgacttccga gcgcagctcg aggatccgct gccagcgggc ggcgatcgcc tccgcgtccg
     1921 ggatcctcag gggcttcggg aggtccgaga ggaagacgct ctccggcttt cccgggtacg
     1981 cggggaggta tccccacgcc tcttccgtgg tgaacgacag caccggggcc gccagcgaca
     2041 gcagtcccct ggcgatttcg aagatcgccg tctgcgcgga gcgccgcgcc gggtctacgg
     2101 cgggggagca gtacatccgg tccttcagca cgttcaggta gaaggacgac atgtccacgg
     2161 agcagaagtt gttgaccgcg tggaagagga cgtggaactc gtactcctcg tacgcattgc
     2221 gcaccttcga cgccagccgg tcgaacagca cgagggcgaa ccggtccatc tcctccatcc
     2281 ggtcgaccgg caccgcgtcc cgcgccgggt cgaagtcgct caggctcgcc agaaggtagc
     2341 ggatcgtgtt gcggatcttc cggtacgctt ccgtcagccg gtcgaggatg tctttcgaca
     2401 accggatgtc gtcccggtaa tcctcggcgg cgacccacag gcggagcagc tccaccccgt
     2461 gcttcttgat gatcccctcc ggggcgatca cgttcccctt cgacttgtgc atcgcctcgc
     2521 ccttgccgtc gacgacgaac ccgtgcgtca gcaccccgcg gtacggcgga attccgcgcg
     2581 tacccacggc ggcgaggaga gaggagtgga accagccgcg gtgctggtcg gaaccttcga
     2641 ggtagaggtc tacggggatg cccaggttct ccttcccctc gcagacgcag gcgtaggaga
     2701 cgcccgagtc gaaccagacg tcgaggatgt cggtctcctt gccgaacgcc gtcccgccgc
     2761 actccgggca cgccgttccg ggcgggagca gttccgacac gccccggtcg aaccaggcgt
     2821 cggccccttc cttctcgaag aagcccgcca cgtgatcgac gagtttccgg tcgagcaggt
     2881 gatgtccgca cccctcgcaa cggaacaggg cgatcggcac gccccaggcg cgctggcgcg
     2941 aaatgcacca gtcggggcga ttcgcgatca tcccctcgat ccgctcctgc ccccaggagg
     3001 ggatccaccg caccttgcgg atctccgcga gggctttttc ccgcaggcgg gtcccgtcca
     3061 tcgagatgaa ccactgcttc gtcgcccgga agatgacggg gctcttgcag cgccagcagt
     3121 gcgggtagga gtgggtgatc tccctgcggg ccatcagcgc gccgacctcg gcgagctttt
     3181 cgttcacctt cggattcgcg tcgaacaccc gctcccccgc gaagaacggg acgtccgcgg
     3241 tgaagcggcc ctcgtcgttc agcggggcga ggatcggcag gccgtacttg agccccgtct
     3301 cgtaatcctc gcggccgtgg ccgggcgccg tgtggacgca cccggtcccc gcatcgagcg
     3361 tcacgtagtc ggccaggacg aggagggagt cgcggtcggc gaacggatgg cggcaccgca
     3421 tccgctcgag gcggcccgcg cggaacgtcg cgaggatcgt cggggaggaa agccccgtct
     3481 cggccgcgaa ccgttccgcg agcccctcgg cgacgacgta cacctcgccg cccgcctcga
     3541 gcgcgacgta gtcgtagtcc gggtggagcg cgatcccgag gttggccggg atcgtccacg
     3601 gcgtggtcgt ccagatgacg aagaagacct ttctccccgc cagcgccggg tggatcctct
     3661 cgggcggctc cacgaacggg aacttgacgt ggatggaggg ggaggtgtgg tccgcgtact
     3721 cgacttcggc ctcggccagc gccgtccggc acgagaggca ccagtagacc ggcttcgtcc
     3781 cctggtacac cgcgccgctc tccacgaacc ggc
//
LOCUS       DASVJL010000271         7626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_324871, whole genome shotgun sequence.
ACCESSION   DASVJL010000271 DASVJL010000000
VERSION     DASVJL010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324871"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1161)
                     /locus_tag="VF357_05630"
     CDS             complement(67..1161)
                     /locus_tag="VF357_05630"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX7523659.1"
                     /translation="MLRRSVLSLTLLLPLLSALCARAEDNVAAVPVESLPAIRIAAVG
                     DIMMGTTFPEPILPPEDGATLFRSVAPLLAGYDVVFGNLEGPLTDVDRSPKCPKPRRD
                     GRPCFAFRTPPRFAARLAEAGFNTVNIANNHALDFGMEGLDNTLAALDNAGIQPVGGV
                     RVAVFTVCGKSVAVAGFSYSLRTRYVHPLRDVEAMRGIVAELKSEYDLVVVSFHGGAE
                     GAQAMRVGDTEEEFMGEQRGNVVRFARAAVDAGADIVLGHGPHVPRAIEVSRGKLIAY
                     SLGNFAVYSMFNIKGPSGLGYVLQVELDPETGDVVRFRTPSVVLHHPGIPHPDPSGKA
                     EALLRNLSEEFLAGEPDAAARRETLSRLWK"
     gene            1235..3100
                     /locus_tag="VF357_05635"
     CDS             1235..3100
                     /locus_tag="VF357_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX7523660.1"
                     /translation="MAFDTTRDVGFGDPLPERDILEGFDRLIAADRLFGAELNSSVIK
                     KSGKGTPFLVRRPPPHHRMLALWRDLFENRVLPKELLDALAPEVLGLVHGDGLLLFGK
                     DRILYDCQIVRGNEGVGHLTLCLYREREPVFRFLPFPRRPGRRIVFIEGITLSAQSTG
                     YASSLFRHYERLFHGLGFHRFRLKASLSVGKYYWAKEGFDCEDRKQFAEMRENLLALV
                     RRLGLPVEEQEVRRLNHANMVATFRRDLLIPVYRNPDGYYATVRDASHTEEFRYPFGK
                     AFLLCSAPWDGYKVIYTDTPRRTGLVWSERFLDHEPGPGHPESPKRLEALLAAIGEEG
                     MRESLIFLEPYIPAMESLRAVHDPAYLEAFREAAARGDRYFAVRDCAISEGSYETALL
                     AAGGVMAGIDAVLTGSSDNAFCAVRPPGHHAGRSTAMGFCFVNNVAAGARYARSAYGI
                     ARIYILDWDVHHGNGTQALFDEDPLTFFCSLHEHPSFCYPGTGRRLEKGAGFGRGATL
                     NIPLSPHAGDREMIEAFEREVVPSIEAFRPELILLSAGFDAHRDDPIAGLECTEESYV
                     HMTRRILELADRHCEGRVISVLEGGYRTESLVSSAIAHIKTLQGREVSPCSSARG"
     gene            3079..3726
                     /locus_tag="VF357_05640"
     CDS             3079..3726
                     /locus_tag="VF357_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HEX7523661.1"
                     /translation="MFVGPRMKRDLVTVTPGETLEAAARLLTAHRIHHLPVVEEGNRL
                     AGIVSDTDLRNATLDGMFGGADRGDSGRPMTVGEIMTRELVTLAPGDTLDDAMLVLTR
                     QRIGALPVVEDDRLVGIVTKADILSALLATLDIEGLGVRIEVVLRRDLKEVARLVAAL
                     ADQDVEVRSLVLALHGADGYAAFIRVATIDVASVRDRLREKGFAVGELSDFYEAR"
     gene            complement(3801..5024)
                     /locus_tag="VF357_05645"
     CDS             complement(3801..5024)
                     /locus_tag="VF357_05645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEX7523662.1"
                     /translation="MADEKLTNRWIMVIAALVMQLCLGVLYSYSVFRGPLMKEVGFTV
                     KQAGYPYMASLFFFAVGMIVAGRWQDKAGPKKVAIFGGVLLAVGAILAGVLYKTVGGL
                     VFSYGILGGLGVGFAYVTPIATCIKWFPDMRGTITGLAVFGFGAGTLVFGPLISKLIA
                     TSGIANAFFAVGVIMLIGVCGAGLMFKVPPAGYKPAGWNPPTPSSTTPTKSDWTPNEI
                     IGNGQFYVLWLIYFFGAAAGLMIIGQAVPIGVEVAKLTPATAAAGLGTMALLNGLGRL
                     VHGSISDKLGRKNTIILCFAEYLVAFLLLLPNADTFTKWLVGLCIVGFSYGGYLAIMP
                     SLTADYFGTKSLGANYGYLFTAWGVAGVGGPFMIDAIKASTGAVTMAMYYISVACVVG
                     IVLVFLSKKPEFKGA"
     gene            5414..5893
                     /locus_tag="VF357_05650"
     CDS             5414..5893
                     /locus_tag="VF357_05650"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX7523663.1"
                     /translation="MTPFQEIKIVPPIKTIGMRGDMSLWDVDTAFGRLYDKALEGRLM
                     FREPAIGVRHGEVRVPDAFRSEYHVLFPLNEQSGVEIPGAEIVNLPGAEVASFVHRGP
                     YEWIQCTYELVLDWLRENRYEAAGEAREVFFVAPEPHSGGSQDDMLTEIQVPIRSAA"
     gene            5922..7049
                     /gene="selD"
                     /locus_tag="VF357_05655"
     CDS             5922..7049
                     /gene="selD"
                     /locus_tag="VF357_05655"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5973..5975,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX7523664.1"
                     /translation="MSDREKIFLTHLSSCAGUASKLGQGVLARVLSALPPPDDPRVLV
                     GTSHADDAGVFRLSGEEALIGTLDFFTPIVDDPYTYGSIAAANSLSDVYAMGGEPLFA
                     LAIAAFPEDEKILPFLADVMVGAADKAREAGICIIGGHTVKDKEPKFGLSVTGRVHPD
                     RMWHNRGAKPGDAIVLTKPLGTGIATSAIKWGICPKETEEAVVASMSRLNANASKAGR
                     EVGISTATDVTGFGLIGHLLEVLDGSGLTAEIRRLDIPVFPGVRELMRRKRVDALADV
                     RPFPGSEWIHGAFGQHPIPGGTHQNMAHQFGRVRLAPMLPAEDAYLLSDPQTSGGLLM
                     FVPEAKAEELCAALCATGEGAWRIGRTFGAGDFEMQRITVI"
     gene            7059..>7626
                     /locus_tag="VF357_05660"
     CDS             7059..>7626
                     /locus_tag="VF357_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7523665.1"
                     /translation="MNILLPVFRGASFDDAVRLAIDTAKAHAGRVLVLSVVDAAEIHR
                     IESGARPGAIHLALHAADEVRKRMTAEGTAAVAGAVRRCEEAGVPAQGEVLEGELERE
                     LVAAAGANDLLVSAVASHFDTDLEDASGQLVLTMMREGGIPVLLSGAPYRPVRTIVAG
                     CGGGTRTERAVGAMVRLSLWKEAPRGILL"
BASE COUNT         1253 a         2477 c         2641 g         1255 t
ORIGIN      
        1 ccgctacggt tcgttggcca tccatggcct cctcacctgc ggaggagcgc aggtgtcgcg
       61 ggcgctctat ttccacaggc gggacaaggt ttcgcggcgc gcggcggcgt cgggttctcc
      121 cgcgaggaac tcctccgaca ggttgcgcaa tagcgcctcc gccttcccgg acgggtcggg
      181 gtgcgggatc cccgggtggt gcaggacgac cgacggggtc cggaaccgga cgacgtcccc
      241 ggtctccggg tcgagctcga cctgcagcac gtaccccagt ccgctcggcc ccttgatgtt
      301 gaacatgctg tagacggcga agttccccag gctgtaggcg atcaattttc cccgggacac
      361 ctcgatggcg cgcgggacgt ggggcccgtg ccccagtacg atgtcggcgc cggcgtccac
      421 cgcggcccgc gcgaaccgga cgacgttccc tcgttgctct cccatgaact cctcttccgt
      481 gtccccgacg cgcatcgcct gcgccccttc ggcgccgccg tggaacgaca cgacgaccag
      541 gtcgtattcg cttttgagct ccgccacgat cccccgcatc gcttcgacgt cccgcagcgg
      601 atggacatac cgcgtccgga gggagtagga gaagccggcc accgccacgc tctttccgca
      661 gacggtgaac acggcgaccc gcaccccgcc gaccggctgg atccccgcgt tgtcgagcgc
      721 ggcgagggtg ttgtccagcc cctccatccc gaaatcgagc gcgtggttgt tcgcgatgtt
      781 caccgtgttg aatcccgcct ccgcgaggcg ggcggcgaat cgcggggggg tgcggaaggc
      841 gaagcagggg cgcccgtcgc ggcgcggctt ggggcacttc ggagagcgat ccacgtccgt
      901 cagcggacct tcgaggttgc cgaagacgac gtcgtacccg gcgagcaggg gcgccacgga
      961 gcggaacagc gtcgcgccgt cctccggcgg caggatcggc tcggggaacg tggtccccat
     1021 catgatgtcg cccaccgccg cgatgcggat cgccgggagc gattccacgg gtacggccgc
     1081 gacgttgtcc tccgcccgag cgcagagggc ggagaggagc ggcaggagaa gggtgagcga
     1141 tagtacggag cgccgcagca tgctctatca tagcaatatg ggtttcaagg tggggaagga
     1201 gtgtccctcg cccacaacca gcgacggagg caggatggcg tttgacacga cgcgcgacgt
     1261 ggggttcggc gatcccttgc cggagcgcga catcctcgag gggttcgacc ggctgattgc
     1321 ggcggaccgg ctcttcggcg ccgaactcaa ctcctcggtc atcaagaaga gcgggaaagg
     1381 gacgccgttc ctcgtccgcc gtcccccgcc ccaccaccgc atgctcgccc tctggcgcga
     1441 cctcttcgag aaccgtgtct tgccaaagga gctcctcgac gcgcttgccc cggaggtcct
     1501 cggccttgtc cacggcgacg gcctcctcct gttcgggaag gaccggatcc tctacgactg
     1561 ccagatcgtt cgcgggaacg agggggtggg acacctcacc ctctgcctct accgggagcg
     1621 cgagccggtg ttccgtttcc ttcccttccc gcgccgcccc ggccgccgga tcgtcttcat
     1681 cgaggggatc actctctccg cacagagcac cggatacgcc tcgtccctct tccgccacta
     1741 cgaacggctc tttcacggcc tgggtttcca ccgtttccgc ctgaaggcct ccctttcggt
     1801 gggaaaatat tactgggcga aggaagggtt cgactgcgaa gaccggaagc agttcgccga
     1861 aatgcgggag aacctcctcg ccctcgtgcg gcgccttgga ctgcccgtcg aggagcagga
     1921 ggtccggcgg ctgaaccacg cgaacatggt ggcgaccttc cggcgagatc tcctgatccc
     1981 cgtctaccgg aacccggacg ggtattacgc caccgtacgg gacgcctcgc acaccgaaga
     2041 gttccggtac cccttcggga aggcgttcct cctctgctcc gctccgtggg acgggtacaa
     2101 ggtcatctac accgacacgc cgcggcgcac gggcctcgtc tggtcggagc ggttcctcga
     2161 ccacgagccg gggccgggac acccggagag cccgaaacgg ctggaggcgc tcctcgccgc
     2221 gatcggcgag gaggggatgc gggaaagcct gatcttcctg gagccgtaca tcccggcgat
     2281 ggagtccctg cgcgcggtgc acgacccggc gtatctcgaa gcgttccggg aggcggccgc
     2341 gcggggggac cggtacttcg ccgtgcgcga ttgcgcgatc tcggagggga gctacgagac
     2401 ggcgctgctg gcggcgggcg gggtgatggc ggggatcgac gcggtcctga cggggagctc
     2461 ggacaacgcc ttctgcgcgg tccgcccccc gggacaccac gcgggacgtt ccaccgcgat
     2521 ggggttctgc ttcgtgaaca acgtcgccgc gggcgcccgg tacgccaggt ccgcctacgg
     2581 catcgcgcgc atctacatcc tcgattggga cgtgcaccac ggcaacggca cccaggcgct
     2641 gttcgacgag gatccgctca ccttcttctg cagcctccac gagcacccct ccttctgcta
     2701 cccggggacg gggcgccgtc tggaaaaggg ggcggggttc gggcggggag ccaccctcaa
     2761 catcccgctt tcgccccacg cgggggaccg ggagatgatc gaggcgttcg agcgggaagt
     2821 cgtcccgtcg atcgaggcgt tccggccgga gctgatcctt ttatccgccg ggttcgacgc
     2881 ccaccgggac gatccgatcg ccgggctcga gtgcacggag gagtcctacg tccacatgac
     2941 gcggcggatc ctcgaactgg ccgaccggca ttgcgagggg cgggtcatct cggtgctgga
     3001 aggcgggtac cgcacggagt cgctggtttc ctcggcgatc gcacatataa aaacattgca
     3061 gggaagggag gtttcgccat gttcgtcggc ccgaggatga agcgcgacct ggtcaccgtt
     3121 acgcccgggg agaccctgga ggcggcggcc cggctgctca ccgcgcaccg gatccaccac
     3181 ctgccggtgg tggaggaggg gaaccgcctt gccgggatcg tctccgatac ggatctgcgg
     3241 aacgcgaccc tcgacgggat gttcggcggc gccgaccgcg gggattccgg ccgtcccatg
     3301 acggtggggg agatcatgac gcgggaactg gtgaccctcg ctccggggga cacgctggac
     3361 gacgcgatgc tggtgctcac ccgccagcgc atcggcgccc tgccggtggt cgaggacgac
     3421 cgcctcgtgg ggatcgtcac caaggccgac atcctttccg ctttgctggc caccctcgac
     3481 atcgaggggc tgggggtccg gatcgaggtg gtcctgcgtc gggacttgaa ggaagtggcc
     3541 cggctggtcg ccgcgctcgc cgaccaggac gtggaggtgc gcagtcttgt cctcgctctc
     3601 cacggggcgg acgggtacgc cgcgttcatc cgggtcgcga cgatcgacgt ggcgtcggtg
     3661 cgggaccgtc tccgggagaa ggggttcgcc gtcggggagc tgtcggactt ttacgaggca
     3721 agataagaag caggaaagga aacggcccct cgaaaggggc cgtttccggt tgacgggttt
     3781 ggttgccggt gccgcgccga tcaggctccc ttgaactccg gcttcttgga gaggaagacc
     3841 agcacgatcc ccaccacgca ggcgacggag atgtagtaca tcgccatcgt gacggcgccg
     3901 gtcgatgcct tgatcgcgtc gatcatgaac gggccgccga cgccggcgac cccccacgcg
     3961 gtgaacaggt agccgtagtt cgcgcccagc gacttcgtgc cgaagtagtc ggccgtcaac
     4021 gagggcatga tcgccaggta tccgccgtag gagaagccga cgatgcagag cccgaccagc
     4081 cacttcgtga acgtatcggc gttcgggagc aggagcagga acgccacgag atattcggcg
     4141 aagcagagga tgatggtgtt cttccggccc agcttgtcgg agatcgaccc gtggaccaac
     4201 cggccgaggc cgttgaggag cgccatcgtg ccgagcccgg ccgccgccgt cgccggggtc
     4261 agtttcgcca cttccacgcc gatcggaacg gcctgcccga tgatcatgag gcccgccgcc
     4321 gcgccgaaga agtagatgag ccagagaacg tagaactggc cgttcccgat gatctcgttc
     4381 ggggtccagt cggacttggt cggcgtcgtc gacgaaggcg tcggcgggtt ccagccggcc
     4441 ggcttgtatc cggcgggcgg gaccttgaac atgaggcccg cgccgcagac gccgatgagc
     4501 atgatcacgc cgaccgcgaa gaaggcgttc gcgatgcccg aggtggcgat gagcttggag
     4561 atcagcggtc cgaagaccag ggtgccggcg ccgaacccga agaccgcaag gcccgtgatc
     4621 gtgccgcgca tgtcggggaa ccacttgatg caggtggcga tcggcgtcac gtaggcgaac
     4681 ccgacgccga ggccgccgag gatcccatag gagaagacca gcccgccgac cgtcttgtac
     4741 agaacgcccg cgaggatagc gcctaccgcg aggagaacac ccccgaagat tgcgaccttc
     4801 ttcggaccgg ctttgtcctg ccatctaccg gcgacgatca tccccaccgc gaagaagaac
     4861 agggacgcca tgtaggggta gcccgcttgc ttgaccgtga acccgacttc cttcatcagc
     4921 ggcccgcgga acacggagta tgaatagaga actcccaggc acaactgcat gaccagcgcg
     4981 gcaataacca ttatccagcg gttcgtcaac ttctcgtcag ccattctgtt tccccctcct
     5041 tccggaatta tcttgtttcg gatcctttga gctttgtccc ctcaatcgcg gtcgatgcgt
     5101 cccgggtcca cctcctttcg ggaagagacg gttttaccct gtaaaataag atttccctat
     5161 cttctacccc aaacgaaaaa ccggtgtcaa gggatttgta aacgcggttt tattaccgct
     5221 tgccggttcg ccttccggat ggccccgccc atcgatcgga gcgccatgcc ggggcgaccc
     5281 gcggaagggc gtgggacatg attcggcgga ggatcggagg cggtgttcgg aaatcgaccc
     5341 ggtgaaggaa tccgggcagg ggagatgggt catctaaagt tgaaagcggt gtcacacgag
     5401 aggagaatcc gacatgaccc ccttccagga gatcaagatc gtccccccta tcaagacgat
     5461 cggcatgcga ggcgacatgt cgctgtggga tgtcgacacc gccttcggac ggctctacga
     5521 caaggcgttg gagggtcggc tgatgttccg tgagccggcc atcggagtgc gccatgggga
     5581 ggtccgggtt cccgacgcct ttcgctccga ataccacgtt ctcttccccc tgaacgaaca
     5641 atccggagtg gagatcccgg gtgccgagat cgtcaacctg cccggggcgg aggtcgcctc
     5701 cttcgtccac cggggcccct acgagtggat ccagtgcacc tatgaattgg tgttagactg
     5761 gctccgggag aaccggtacg aagccgcggg ggaggcacgc gaggtcttct tcgtagcgcc
     5821 tgagccgcac tcggggggaa gccaggacga catgctgacc gagattcagg tccctatccg
     5881 ttccgcggcg tgaaccggct ccccgggagg atgggggagg cgtgagcgat cgggagaaga
     5941 ttttcctgac ccacctgtcg tcctgcgccg gttgagcgtc caagctgggg cagggggtcc
     6001 ttgcccgcgt gctgtccgcc ttgccgccgc ccgacgatcc gcgtgtgctc gtgggcacct
     6061 cccacgccga cgacgcgggg gtgtttcgcc tctcggggga agaggctctc atcggaaccc
     6121 tcgacttctt caccccgatt gtcgacgatc cgtacacata tggctcgatc gcggccgcca
     6181 actccctgag cgacgtgtac gcgatggggg gggagccgct cttcgccctc gccatcgccg
     6241 ccttccccga ggacgagaag atcctgccgt tcctcgcgga cgtgatggtc ggcgcggcgg
     6301 acaaggcgag ggaggccggc atctgcatca tcgggggcca cacggtgaag gacaaggagc
     6361 cgaagttcgg cctctcggtg accggccggg tccaccccga ccggatgtgg cataatcgcg
     6421 gggcgaagcc cggtgatgcg atcgtcttga cgaagcccct ggggaccggc atcgccacct
     6481 cggcgatcaa gtgggggatc tgcccgaagg agacggaaga ggcggtggtc gcttcgatga
     6541 gtcgactgaa cgcgaatgct tcgaaagcgg ggcgggaggt cgggatctcc acggccaccg
     6601 acgtcaccgg gttcggtttg atcggacacc tgctggaggt gctcgacggg agcgggctga
     6661 ccgccgagat ccggcgtctg gacattccgg tcttccccgg ggtacgggag ctgatgcggc
     6721 gcaagcgtgt cgacgccttg gccgacgtga ggccgttccc ggggtcggaa tggatccacg
     6781 gcgccttcgg gcagcatccg atccccgggg gaacccacca gaacatggcg caccagttcg
     6841 gacgcgtccg cctcgccccc atgctgccgg cggaagacgc gtatctcctg tccgacccgc
     6901 agacctcggg ggggctgctg atgttcgtcc ccgaggcgaa agccgaggaa ctgtgcgccg
     6961 ccctgtgcgc cacgggcgag ggggcgtggc ggatcggccg gacgttcggg gcgggcgact
     7021 tcgagatgca gcggatcacg gttatttgag gtgacggcat gaacatcctg ctccccgtgt
     7081 tccgcggcgc ctcgttcgac gacgccgtgc ggctggcgat cgacacggcg aaggcgcacg
     7141 ccggccgggt cctcgtcctt tccgtggtgg acgcggcgga gatccaccgg atcgagtcgg
     7201 gtgcgcggcc cggggcgatc cacctggcgc tgcacgcggc cgacgaggtg cgcaagcgga
     7261 tgacggccga ggggacggcc gcggtcgccg gggcggtgcg ccgatgcgaa gaggcgggcg
     7321 tccccgccca gggtgaggtc ctggaggggg agctcgagcg ggagctggtg gcggcggccg
     7381 gggcgaacga cctgctcgtc tcggcggtgg cgtcccactt cgataccgac ctcgaggacg
     7441 catcggggca gctcgtcctc acgatgatgc gggagggggg gatccccgtt ctgctctccg
     7501 gagctccgta tcgtcccgtg cgcacgatcg tcgccggctg cgggggaggg acccggaccg
     7561 agcgtgcggt gggggcgatg gtccggctct cgctgtggaa ggaggccccc cgcgggatcc
     7621 tcctcg
//
LOCUS       DASVJL010000272        12547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_42708, whole genome shotgun sequence.
ACCESSION   DASVJL010000272 DASVJL010000000
VERSION     DASVJL010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 12547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12547
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42708"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /gene="lnt"
                     /locus_tag="VF357_05665"
     CDS             complement(<1..1033)
                     /gene="lnt"
                     /locus_tag="VF357_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX7523666.1"
                     /translation="MNRRGPAALFLTGALFVLSYALGTPGYDVPGLPLVCLAPFLALA
                     SSARSARQAAWRGWIAGTAGNVPLYYWIAYTIAVQGRLGWALGALAAFLVSAYVGAYF
                     SVAAAAARHLESRFGEAGLWLFPFVWTAVEMARSHLFSGFPWMLLGYTLAGSATLRQA
                     ADLAGVHGLSFLLALSGVSVYLVGRRLSDRFQAKAAIPLIPGVAAILFLVLYGRAGSA
                     NPAGPADRIPAVKVAIAQGGIDQSVKWDPGNQIATLEIYGELTRKAREAGAQVVVWPE
                     TAAPFFYGWEAELSRRLEAIAVSGGIPIIFGAPWFEPSGGGKFYNSVFQMDARGVVLG
                     RYDKRHLVPF"
     gene            complement(1030..1497)
                     /gene="ybeY"
                     /locus_tag="VF357_05670"
     CDS             complement(1030..1497)
                     /gene="ybeY"
                     /locus_tag="VF357_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX7523667.1"
                     /translation="MNRYRVNVRQDLRPAPFPGRRLKAYARAALALLSANGADVRIRV
                     VGDAAIARWNRKFLGRDLPTNVLSFPEADGVPVRGARVKGDIVVSAPTCLSETGGWKE
                     APEARVFFFVLHGILHLAGYDHESGGAPARRMRRKELALYRRVLDGEGRGRTR"
     gene            complement(1494..2996)
                     /locus_tag="VF357_05675"
     CDS             complement(1494..2996)
                     /locus_tag="VF357_05675"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX7523668.1"
                     /translation="MSGSKNATRNSSRPPENGPSPRGRYWRPLALSLGSGAVFAVLFY
                     LWGRELFPRAAGRWGETFPFVGAVALTLAVALFLSEWFGFAGREVRKVRLAARDFLFL
                     CSLALLLFFAAKAVIDLLAEIPAIGRSVPTRVYSYLIPLPAFAMVVRVLINSETSILF
                     TVAASVLSAAAASGSWPALLFLLLSGTAGAARARRVPDRYRMLIAGVQTAPVCALAAA
                     ALEFSFHGDGGVLWAGIFGAVNGLLSGPIALAVMPVAESAFGYASDIRMMELSGAGHP
                     LLSRLMLETPGTFHHSVVVGTLAEAGALAIGANPVLCRVAALYHDIGKLGKAQYFSEN
                     QAGKENIHDAMSPGLSRAVILSHVKEGVRIAEEFRLGDRITEIIAQHHGTSLLYCFLD
                     KARPMAQDRKALEEMFRYPGPKPKTREAAIIMLADATEAAVRSLDRPTPQEVDDTVTG
                     IVNRIYLDGQLNECDLTLRDLHAVGRAISQVLAAVAHARVDYPEAGKGAT"
     gene            complement(2962..3972)
                     /locus_tag="VF357_05680"
     CDS             complement(2962..3972)
                     /locus_tag="VF357_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7523669.1"
                     /translation="MGRLLLMREASVVNDAMEETIRFEDASVLRDLFGVNDAHLRALE
                     RGLGVTIRPGGNEARVAGDRIPVELAGKVLSGLYRVLRRGIPVIGNDVEAAIRIVSAD
                     RNADVSVVFEDVVFKSSRNRTITPKSVAQKRYLDAIREFAIVFGVGPAGTGKTFLAMA
                     MAVAYLLRKEVKRIVLVRPAVEAGERLGFLPGDMAEKVNPYLRPLHDALYTMLEFEQA
                     AKLMERGTIEVAPLAFMRGRTLNDSFVILDEAQNTTSEQMKMFLTRIGFGSRAVITGD
                     ITQTDLPSGKVSGLNEAIAILDGVAGIRFCRFTDIDVVRHPIVQEIIKAYERFEKRDQ
                     EQ"
     gene            complement(3954..4841)
                     /locus_tag="VF357_05685"
     CDS             complement(3954..4841)
                     /locus_tag="VF357_05685"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX7523670.1"
                     /translation="MQGKIADFSIPDIFQLVSSQGKSGSLAISGEDRVTIFLFSGGRI
                     VDVQPDRREPKSLLGTMLRDAGYLTDSELKRFLSSQGAGGKKIGEIVVEKGKVSKEVL
                     ARYLVLQVKESLFDVLTLREGEYRFEGFAVRPVAWGGEPIRPDVLMMEGMQFLDEYPR
                     FREIFPAGDFRVARKKGERVDMYALTEEERVLWKALDFSEDPDRVYRKACLTGFEGIK
                     ALAALLQRGLIEVSTTAPGDLVDPVQRLRDEIAFRRRFDAIRMALWGAAMAAAAVWVY
                     RGLLSPGAFRTFTVWVDFF"
     gene            complement(4844..6757)
                     /locus_tag="VF357_05690"
     CDS             complement(4844..6757)
                     /locus_tag="VF357_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523671.1"
                     /translation="MTRRVPKRSRRSWTRFALFLLLLFPLVPSRLAAADLFPGYPEPV
                     REQAIRVVEAAGPGKGEALEREVRALRRAMFDNAILSMNAVPDRIFDRAAREGWKRGS
                     YESIRAVARVAPLSVPLWSWLVWEDAVRFRLEEFLFDVKGLSGALRQYGPGLLGYAVW
                     FVLFASASACWFAVWASLNLLLRALPALTSDVARLLKGLPRPEIFASGIVLAGFAVPV
                     ACGVGIAAAAVFWIALSTAYLRRGELVIGGMAILLLVGVFLCGGFLEAIRPVAGMARQ
                     GGWLVGEGYYYRGGPGSGDPGQGLLTGTKWERMARFARARAEMEGGNLRTADSLWTEQ
                     IEEGEDLSDAYNNRGIVRVRLGRTEEGLADFEAAASLSPTGGRAQWNAYQLYLQEFRL
                     AQAARIQPVAWVGIRGLVPFDYRAEEMTHGELVAAPLRVGDVWRSLFTVREEWFREAR
                     RSAFHALFFRPVPGEWVMAFLAAGLAWEALWKLLSRKIWMSSVCRSCGAGTMVVGSRE
                     TIDICNACRAQVGMGIRGGEESERRGLSISLHRRYVRACSVLFPGAGALWAGKEFRTM
                     LYGILLSLSAGLFTVSWSAGRLAGGLIGDMQTDIWRAALGVFAVLWMAGAAWGWRSFE
                     NLQMNLNVAGERF"
     gene            6797..7570
                     /gene="mazG"
                     /locus_tag="VF357_05695"
     CDS             6797..7570
                     /gene="mazG"
                     /locus_tag="VF357_05695"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX7523672.1"
                     /translation="MDEFRKLVEIMARLRANGGCEWDRAQTHETLRQYLVEETHEVID
                     AIRGGNPDSLCEELGDLLLQILFHAQIASENGQFDIIDVIASISEKMVRRHPHVFGDA
                     TADTPEAVSRQWDHIKKTIENRSRESIIGGIPKEFPSLLRAAKMSKKAARAGFDWERT
                     EQVMGKVEEELSELKEAMAEGNPAHTEHELGDVLFSLVNLARFLGLNAEVAMVSVNER
                     FERRFREMEKIAAETGCSIEAADMPTLDRLWEMAKKSTT"
     gene            7775..8044
                     /gene="rpsT"
                     /locus_tag="VF357_05700"
     CDS             7775..8044
                     /gene="rpsT"
                     /locus_tag="VF357_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX7523673.1"
                     /translation="MARIKSGIKRHKQSLKARARNRHVRSTLKTAVKEVRSEIAKGTG
                     EKIAELLRKATSVLERAGSKGVLHKKTASRKVSRLAKAVHKAAKK"
     gene            complement(8046..8966)
                     /locus_tag="VF357_05705"
     CDS             complement(8046..8966)
                     /locus_tag="VF357_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523674.1"
                     /translation="MKPADRPPIFREWLSVDPAPAYLLYGDGAGLAGLLARSWEGKLR
                     GEGVPIETFRWTLEDLARESPTAAWRSPSFFSRLRIFTLPDLAEMKKVHRDEIKTYLE
                     FPEPSAMLILHGTDFRQARGFSSARNLLSDAPREEKAVEALARHAVAAAAEGGAKLTR
                     ESALFLARWVGASFDALDAEIGKVLAFAAGRGEVVEEDIRAVCVSRGDVDPFRLAETL
                     VRKDSKACLALFRRFAAAAEPDDYHKLNGAVAWFLRSRIQGKGGAGGVTPRRAAEIFR
                     TLSRIDREMKGESRLSPGQVYEIRLLSLLT"
     gene            complement(8963..9472)
                     /gene="lptE"
                     /locus_tag="VF357_05710"
     CDS             complement(8963..9472)
                     /gene="lptE"
                     /locus_tag="VF357_05710"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX7523675.1"
                     /translation="MKRILIVLALLSAVAGCGYRLESGTARFKDPSVRMDVSSFANRS
                     TTPDAGAVVAARFREELRRSGFRGSFGKIGANYLIEGTVRDVRSEIFSHGADRFSLEN
                     RLTLVVDIRVVEIVRGGVLWKESDLSETASYFAGADAQYTEANRRAAFEQVVRRMAVR
                     LSQTLRVLL"
     gene            complement(9473..12049)
                     /gene="leuS"
                     /locus_tag="VF357_05715"
     CDS             complement(9473..12049)
                     /gene="leuS"
                     /locus_tag="VF357_05715"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX7523676.1"
                     /translation="MKYKPQEIERKWQQRWNEAGVSRCPDELSAPKYYCLEMFPYPSG
                     RIHMGHVRVYTIGDLLARFKRMQGFQVLHPIGWDAFGLPAENAAHRHGTHPAKWTWEN
                     IAFMREQLKEMGISYDWDREFATCSPEYYKWEQLFFLWMLRDGLAYRKRATLNWCGEC
                     QTVLANEQVNRDGTCFIHDHTPVAQKELDQWFIGITKYAEELLSGHKELEGRWPANIL
                     EMQRNWIGRSEGAEIRFPLDGGGGDVTVFTTRPDTLFGATFMSMAPEHPMVMEFAKRT
                     GREREVREFVDRVASQDRIARTSEDLVKEGVFIGGYCVNPVTGNRMPVYAANFVLYDY
                     GTGAVMAVPAHDQRDFEFARKYGLPVAVVGQPEGEALDPATMTAAHEGPGRLVGSGAF
                     DGISSEEGKKAITLHLEGKGMGRGTIQYRLRDWGVSRQRYWGCPIPVIHCDACGIVPV
                     PEKDLPVVLPEDLPYTRERGNPLAGAEHWLRVPCPSCGKPGRRETDTFDTFVESSWYF
                     LRFIDPKNDRAPLDPEKMRRFAPVDQYVGGVEHACMHLIYARFFHKYLRDKGLTPGNE
                     PFERLLSQGMVCMQTAECPKHGWRYPEEVDEKGKCRQCGEAVDVGRSMKMSKSKRNVV
                     EPSALIERYGADTARLFILFAAPPEKDLDWNEQGVEGAFRFLNRIYRLVAPRAKAIAD
                     ATAAWDGTEEVRAIRQVTHRTLIKVTGDIEDRSHFNTAISAVMEMVNFLYLVPEAAWG
                     RPASAAALREAVEILLHMLSPFAPHIGEELWERIGGEGLLCSRSWPAADVDVAREEEV
                     EVVIQVNGKVRSKLTVGAGAGEGEVRERVMADPKVREYTAGKEIRRTVYVPGKLFSIV
                     VS"
     gene            complement(12046..12468)
                     /gene="nusB"
                     /locus_tag="VF357_05720"
     CDS             complement(12046..12468)
                     /gene="nusB"
                     /locus_tag="VF357_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX7523677.1"
                     /translation="MRRESREKVFQTLFMMDALGVGPDEAIPLFALASDPPSDAGYYA
                     EAVRGVWGRIGEIDALIGEAAEHWRVGRMTLVDRNILRLGAYELSLGSDIPFAVAINE
                     AVELGKRFGSEESGAFINGILDRISEIVRNTATAEGSA"
BASE COUNT         2255 a         4364 c         3961 g         1967 t
ORIGIN      
        1 tgaacggcac caggtggcgc ttgtcgtacc gcccaaggac gaccccgcgc gcgtccatct
       61 ggaagacgct gttgtagaat ttcccgcccc ccgagggttc gaaccagggc gccccgaaga
      121 tgatcgggat ccccccgctc accgcgatgg cctcgagccg ccgggagagc tccgcttccc
      181 acccatagaa gaacggagcc gcggtctccg gccagacgac cacctgcgcg cccgcctccc
      241 tcgcctttcg cgtcagctcc ccgtagattt cgagcgtggc aatctggttt ccgggatccc
      301 acttcacgga ctggtcgatc ccgccctggg cgatggccac cttcaccgcg ggaatccggt
      361 ccgcggggcc tgccgggttc gccgaaccgg cccgtccgta cagaaccagg aacaggatcg
      421 ccgcgacccc ggggatcagc gggatcgccg ctttcgcctg gaaacgatcg gacaacctcc
      481 tccccacgag gtagacggag acgcccgaga gagcgaggag gaaggagaga ccgtgaactc
      541 ccgccaggtc cgccgcctgg cgcagcgtcg cgctccccgc gagggtgtac ccgaggagca
      601 tccaggggaa cccggagaaa aggtggcttc gtgccatctc cacggcggtc cagacaaagg
      661 gaaacaacca aaggcccgcc tcgccgaacc ggctctccag gtggcgcgcg gcggcggcgg
      721 cgaccgaaaa gtaggcgccg acataggcgg agacgagaaa tgccgccaac gctccgagcg
      781 cccagccgag ccttccctgc acggcgatgg tgtaggcgat ccagtagtag agggggacgt
      841 tgccggccgt tcccgcgatc catccgcgcc aggcggcctg ccgcgccgag cgggccgaag
      901 acgcgagcgc caggaacgga gcgaggcaga cgaggggcag tccgggcacg tcgtaccccg
      961 gggtcccgag cgcgtaggaa agaacgaaca acgcgccggt caggaagagt gccgccgggc
     1021 cccggcgatt caacgggtcc gcccccggcc ttccccgtcg aggacccgcc ggtacagcgc
     1081 cagttccttc cggcgcatcc ggcgcgccgg cgcgccgccg ctctcgtggt cgtaccccgc
     1141 aaggtggagg atgccgtgga ggacgaagaa gaaaacgcgc gcctccggag cctccttcca
     1201 tccccccgtc tccgaaaggc aggtgggcgc ggaaacgacg atgtctccct ttactcgcgc
     1261 gccgcgcacc gggacaccgt ccgcctccgg gaaggagaga acgttcgtgg gaaggtcccg
     1321 gccgagaaat ttccggttcc agcgcgcgat cgcggcatcc ccgaccaccc ggatccgcac
     1381 atcggccccg ttcgcggaaa ggagagcgag cgccgcgcgg gcatacgcct tcaaccgccg
     1441 cccgggaaac ggcgcggggc gaaggtcctg gcgaacgttg acccggtatc ggttcaagtc
     1501 gctcccttgc cggcttcggg gtagtcgacg cgggcgtggg cgacggcggc gaggacctgg
     1561 ctgatggcgc ggccgacggc atgcaggtcg cgcaaggtca ggtcgcactc gttcagctgg
     1621 ccatcgaggt agatccggtt gacgatcccg gtgacggtat cgtccacttc ctgcggcgtg
     1681 gggcggtcaa ggctcctcac cgccgcctcg gtcgcgtcgg ccagcatgat gatcgccgcc
     1741 tcgcgggtct tcggcttcgg ccccgggtaa cggaacatct cctccaacgc cttccggtcc
     1801 tgtgccatcg gcctcgcctt gtcgaggaag cagtacagca ggctcgtccc gtggtgctgc
     1861 gcgatgatct ccgtgatccg gtcccccaac cggaattcct ccgcaatgcg gaccccttcc
     1921 ttcacgtggg acaggatcac cgcgcgggag agcccgggag acatcgcgtc gtgaatattc
     1981 tccttgccgg cctggttctc ggaaaaatat tgggccttcc cgagcttccc tatgtcgtgg
     2041 tagagagcgg cgacgcggca aagcaccggg ttggcgccga tggcgagcgc gccggcctcc
     2101 gccagcgtcc cgaccaccac gctgtggtgg aatgtccctg gcgtctccaa catcaggcgg
     2161 gagaggagcg ggtgaccggc gccggacagc tccatcatgc ggatgtcgct cgcgtagccg
     2221 aaggcggact ccgccaccgg catgaccgcg agagcgatcg gcccggacag gaggccgttg
     2281 acggcgccga agatcccggc ccacaatact cccccgtcgc cgtggaacga gaactcgagg
     2341 gccgccgcag cgagggcgca cacaggtgcg gtctggacgc cggcgatcag catccggtac
     2401 cggtccggca cccggcgcgc gcgcgcggcg ccggcggtcc cggacaggag gaggaacagc
     2461 agcgcgggcc agcttcccga ggcggcggcg gccgagagga ccgacgcggc gaccgtgaag
     2521 aggatcgacg tctcggagtt gatgaggacc cggaccacca tcgcgaaggc ggggagcgga
     2581 atcagatagg aatacacccg cgtcgggacg ctgcgcccga tcgccggaat ctcggccagc
     2641 agatcgatca ccgccttcgc cgcgaagaac aggaggagag cgagggagca gaggaagagg
     2701 aagtcccgcg ccgccaaccg gacctttcgg acctcgcggc cggcgaaccc gaaccattcc
     2761 gagagaaaga gggccacggc tagcgtgagc gcgaccgcgc cgacgaaggg gaaggtctcc
     2821 ccccatcgcc ccgccgccct cgggaagagt tcccgccccc agagatagaa gaggaccgcg
     2881 aagacggcgc ccgacccgag actcagcgcg aggggcctcc agtagcggcc ccgcggcgac
     2941 ggcccgttct ccggcggacg gctactgttc ctggtcgcgt ttttcgaacc gctcatacgc
     3001 cttgatgatc tcctggacga tggggtgccg gacgacgtcg atgtcggtga accggcagaa
     3061 acggattccc gccaccccgt cgaggatggc gatcgcctcg ttcagtcccg aaacttttcc
     3121 cgatggaaga tccgtctgcg tgatgtcccc cgtgatcacc gcccgggagc cgaagccgat
     3181 ccgggtgagg aacatcttca tctgctcgga ggtcgtgttc tgcgcctcgt cgaggatgac
     3241 gaaggagtcg ttcagcgtcc gcccccgcat gaaggcgagc ggggccacct cgatcgtccc
     3301 ccgctccatg agcttcgccg cctgttcgaa ctcgagcatg gtgtacaggg cgtcgtggag
     3361 ggggcgcagg tacggattca ccttttccgc catgtccccg gggaggaagc cgagccgctc
     3421 ccctgcctcc accgcgggcc ggaccaggac gatccgtttc acctccttgc gcaggaggta
     3481 cgcgaccgcc atcgccatcg cgagaaacgt tttccccgtc ccggcggggc ccacgccgaa
     3541 gacgatggcg aactcccgga tggcgtcgag gtaccgcttc tgcgcgacgc tcttcggcgt
     3601 gatcgtccgg ttccgcgacg acttgaacac cacgtcctcg aacacgacgg acacgtcggc
     3661 gttcctgtcg gcggagacga tgcggatcgc cgcctcgacg tcgttcccga tgacggggat
     3721 gccgcggcga aggacccggt acagaccgga caggactttc cccgccagtt ccaccgggat
     3781 cctgtccccc gcgacgcgcg cctcattccc cccgggccgg atcgtcacgc ccagtccccg
     3841 ttccagggcc ctcagatggg cgtcgttcac tccgaagagg tcccgcagaa cggaggcatc
     3901 ctcgaaccgg atcgtctctt ccatcgcgtc gttcactacg ctcgcctccc gcatcagaag
     3961 aagtcgaccc acaccgtaaa ggtccggaac gccccggggg agaggagccc ccggtagacc
     4021 catacggccg ccgcggccat cgccgcaccc cacagcgcca tccggatcgc atcgaacctg
     4081 cgccggaatg cgatctcgtc gcgcagtcgc tggacgggat cgacaaggtc cccgggcgcc
     4141 gtcgtggaga cctcgatcag cccccgttgg aggagagcgg ccaacgcctt gatcccctcg
     4201 aatcccgtca gacacgcctt ccggtaaacg cggtcgggat cctccgagaa gtcgagggcc
     4261 ttccataaaa ccctttcctc ctcggttaat gcgtacatgt cgacgcgctc ccccttcttc
     4321 cgcgctaccc gaaaatcacc tgcggggaat atttcgcgaa atcgaggata ttcatcgagg
     4381 aattgcatcc cctccatcat gaggacatcc ggacgaatcg gttcccctcc ccacgcgacg
     4441 ggacgtacgg cgaacccctc gaaccgatac tccccctcgc gaagcgtgag aacgtcgaag
     4501 aggctctcct tgacctggag gacgaggtaa cgggcgagga cctctttcga gaccttcccc
     4561 ttctcgacca cgatttcgcc gattttcttt ccaccggccc cctgggatga caggaaccgc
     4621 ttgagctcgg agtccgtcag gtaccccgca tcccggagca tcgtgccgag gagggatttc
     4681 ggttcacgcc ggtccggctg gacatcgacg atccttcctc cggagaagag aaagatcgtc
     4741 acgcgatcct ccccggagat cgcgagggaa ccgctcttcc cttgggagga gaccagctgg
     4801 aagatatccg ggatgctgaa gtcggcgatc ttcccctgca tcgctaaaac ctttccccgg
     4861 cgacgttgag attcatctgg aggttctcga acgagcgcca tccccaggcg gccccggcca
     4921 tccaaagaac cgcgaacacc cccagcgccg ccctccatat atcggtctgc atgtcgccga
     4981 tcaggcctcc cgccaacctg ccggcgctcc acgaaacggt gaacaggccg gcggacaggg
     5041 agaggaggat gccgtacagc atggtccgga actccttccc cgcccagagg gctcccgccc
     5101 ccgggaacag gacggagcag gcgcggacat acctgcggtg cagcgaaatg ctcaaccccc
     5161 gccgctcgct ctcctccccg ccccggatcc ccatgccgac ctgagcacgg cacgcgttgc
     5221 agatgtcgat ggtttcccgg ctcccgacga ccatcgtgcc ggcgccgcac gaacggcaga
     5281 cgctgctcat ccagattttc cgggagagaa gtttccacag cgcctcccag gccaacccgg
     5341 ccgccagaaa cgccatgacc cattctccgg gaacggggcg gaagaacagg gcgtggaacg
     5401 cgcttctccg ggcctcccga aaccactctt cccgcacggt gaacaggctc ctccacacgt
     5461 cgccgacccg cagcggagcg gcgaccagct cgccgtgggt catctcctcg gcgcggtagt
     5521 cgaaggggac gaggccgcgg attccgaccc aggcgaccgg ctggatccgg gcggcctgcg
     5581 ccagccggaa ctcctgcagg tacaactggt aggcgttcca ttgtgcccga ccgcctgtcg
     5641 ggctgaggga agccgcggct tcgaaatcgg ccaacccttc ctccgtcctt ccgaggcgga
     5701 cccggacgat cccgcggttg ttatacgcgt cggagagatc ctctccctct tcgatctgtt
     5761 cggtccagag cgagtccgcc gtccgcaggt ttcccccctc catctcggcc cgggcgcggg
     5821 cgaaccgcgc catgcgctcc catttcgtcc cggtcagcag cccttgtccg ggatccccgg
     5881 agcccggccc gccccggtag taatacccct cccctaccag ccatcctccc tgtcgcgcca
     5941 ttcccgccac cgggcggatc gcctcgagga agccgccgca aaggaagacg ccgaccagca
     6001 gcaggatcgc catccccccg atcacgagct cgccgcggcg caggtacgcg gtggagagcg
     6061 cgatccagaa gactgcggcg gcggcgatcc cgaccccgca cgcgaccgga accgcaaacc
     6121 cggcgaggac gatcccggag gcgaagatct ccgggcgggg gagacccttg aggaggcggg
     6181 cgacgtccga cgtcagcgcg ggaagcgccc gcaggaggag attgagcgac gcccaaaccg
     6241 cgaaccagca cgccgaggcg gaggcgaaga ggacgaacca cacggcgtac ccgagcagcc
     6301 cgggaccgta ctggcgaagc gctcccgaca accctttgac gtcgaagagg aactcttcga
     6361 gccggaaccg caccgcgtcc tcccagacaa gccaggacca gagggggacg gatagcggcg
     6421 ccacgcgcgc caccgctctg atcgactcgt acgacccgcg tttccatcct tccctggccg
     6481 cgcggtcgaa gatccggtcc ggaacggcgt tcatggaaag gatcgcgttg tcgaacatcg
     6541 cgcgccgaag ggcgcggacc tcccgctcaa gcgcctcccc cttcccggga cccgccgcct
     6601 cgacaacgcg gatggcctgt tcgcgaacgg gttccggata tccgggaaaa aggtccgccg
     6661 cggcaagccg ggagggaacg aggggaaaca ggagcaggag gaagagcgcg aaacgcgtcc
     6721 acgacctccg ggatcgcttg ggaacacgtc gagtcataat cctttatgct aacattagtc
     6781 tggagaaaaa actctcgtgg acgaattccg taaactggtg gaaatcatgg cccgccttcg
     6841 cgcgaacggc ggctgcgagt gggaccgcgc ccagacccac gaaacgcttc gccaatatct
     6901 cgtggaagag acgcatgagg tgatcgacgc gatccgtggc ggaaacccgg attcgctgtg
     6961 cgaagagctg ggggaccttc tcctgcagat ccttttccat gcgcagatcg cctccgaaaa
     7021 cgggcagttc gacatcattg acgtgatcgc ctccatctcg gaaaagatgg ttcgccgaca
     7081 cccgcacgtc ttcggggacg cgaccgccga cacgccggag gcggtatcgc gacaatggga
     7141 tcacatcaag aagacgatcg agaaccgctc ccgggaatcg atcatcggag ggatcccgaa
     7201 agagtttccc tcccttctcc gcgcggcgaa gatgtcgaaa aaggcggcga gggcggggtt
     7261 cgactgggag cggacggagc aggtgatggg aaaggtcgag gaggagctct cggaactgaa
     7321 agaggcgatg gcggagggga atccggccca cacggagcac gaactcggcg atgttctgtt
     7381 ctccctggtg aatctggccc gattcctcgg cctgaacgcc gaggtggcga tggtttcggt
     7441 caacgaacgg ttcgagcgcc ggtttcgaga gatggaaaaa atcgcagcgg aaacaggatg
     7501 ttcaatcgag gctgccgata tgcccaccct ggatcgcctc tgggagatgg cgaagaagtc
     7561 gacgacctga ccatcccggc ttattccaca tcctttgtgg actgtctgtg gattctcctg
     7621 tggaaattcc ccctcgcccg tgtccgtgag ccccctccat cagaatgcat ttttattggg
     7681 catccggggg cgtgtatttc tttgacattt catccctccc ggtgtctaat accgctttac
     7741 cgaaatcatc gaacggatcg caaggaggca caggttggca aggatcaagt ccgggatcaa
     7801 acgtcacaag cagagcctga aagccagggc ccgcaaccgc cacgtccgct ccaccttgaa
     7861 gaccgccgtc aaggaagtcc ggtccgaaat cgccaaggga accggggaga agatcgccga
     7921 actgctgcgg aaagccacgt ccgtcctcga gcgcgccgga tcgaagggcg tccttcacaa
     7981 gaagaccgcg tcccgcaagg tctcgcgcct ggcgaaagcc gtccacaagg cggcgaagaa
     8041 gtaacctagg tcagaagcga cagcagccgg atctcgtaca cctgcccggg ggagagcctg
     8101 ctctccccct tcatctcgcg gtcgatccgt gacagggtcc ggaagatctc cgctgcccgc
     8161 cgcggcgtga cgccgccggc accccccttc ccctggatcc tgctccgaag gaaccacgcc
     8221 accgccccgt tcagcttgtg gtaatcgtcg ggctccgcgg cggccgcgaa gcgacggaac
     8281 agcgcgaggc acgccttgct gtccttccgc accagcgtct cggccagccg gaacgggtcc
     8341 acgtctcccc tggaaacaca caccgcccgg atgtcttcct cgaccacttc cccgcggccg
     8401 gcggcaaacg caagcacctt cccgatctcc gcgtcgaggg cgtcgaagga ggcccccacc
     8461 caccgcgcga ggaagagcgc cgattcccgc gtgagcttcg cgccgccttc ggcggccgcc
     8521 gctacggcat gtctcgccag cgcctcgacc gccttctcct ccctgggggc gtccgacagg
     8581 aggttccggg cgcttgagaa cccgcgcgcc tggcggaaat cggtcccgtg gaggatcagc
     8641 atcgcggaag gttccgggaa ttcgagatac gtcttgatct cgtcccggtg caccttcttc
     8701 atctcggcga ggtccgggag cgtgaagatc cgcaagcggg agaagaacga cggggatcgc
     8761 cacgccgcgg tgggggactc ccgggcgagg tcctcgagcg tccaccggaa cgtctcaatc
     8821 ggaaccccct cgccgcgcag cttcccctcc cacgacctcg cgagaagtcc ggcgagcccg
     8881 gcgccgtctc cgtacaggag gtatgccggg gcgggatcga cggagagcca ctcccggaag
     8941 atcgggggac gatcggccgg tttcaaagga gcacgcgaag ggtctgggac aagcgcacgg
     9001 ccatccgccg gacgacctgc tcgaaggcgg cgcgccgatt ggcctccgtg tactgcgcgt
     9061 ccgcacccgc aaagtaggaa gcggtctcgc tcaggtcgga ctctttccag agcacccctc
     9121 cccggacgat ctcgaccacc cggatgtcta ccaccaaggt gaggcggttc tccagcgaaa
     9181 accggtccgc gccatgggag aagatttccg aacggacatc gcgcaccgtt ccctcgatca
     9241 ggtagttcgc gccgatcttt ccgaaggaac cccgaaagcc gctgcggcga agttcctcgc
     9301 gaaagcgcgc ggcgacaacg gcaccggcgt cgggcgtcgt ggaacggttc gcgaaggagg
     9361 agacatccat tcgcaccgag ggatccttga atcgcgccgt cccggactct aggcggtatc
     9421 cgcatcccgc gaccgcggac aggagcgcca acacgatgag aatccgtttc atttacgaaa
     9481 cgacgatgct gaagagtttg ccgggaacgt agacggtcct ccggatctcc ttgcccgccg
     9541 tgtattcccg gaccttcggg tcggccatca cccgctcgcg gacctcgccc tctccggcgc
     9601 cggctccgac ggtcagcttc gaccggacct tcccgttgac ctggataacg acctcgacct
     9661 cctcttcgcg cgcgacgtcg acatcggcgg cgggccagga acgggagcag aggagtccct
     9721 ctcctccgat ccgctcccag agttcctcgc cgatatgcgg cgcaaaggga gagagcatat
     9781 ggagcaggat ctccaccgct tcgcggagcg cggcggccga cgcgggacgc ccccacgcgg
     9841 cctcgggaac gaggtagagg aaattcacca tctccatgac ggcggagatc gccgtgttga
     9901 agtgggaccg gtcctcgatg tcgccggtga ctttgatcag cgtacggtgg gtcacctggc
     9961 ggatggcgcg gacctcctcc gtgccgtccc acgcggccgt ggcgtccgcg atcgccttcg
    10021 cgcgcggggc gaccagccga tagatgcggt tgaggaaccg gaacgctccc tccaccccct
    10081 gttcgttcca gtcgaggtct ttttccggcg gcgccgcgaa gaggatgaag agtcgcgccg
    10141 tgtcggcccc gtaccgctcg atcagcgcgg acggctcgac gacgttccgc ttcgacttgg
    10201 acatcttcat cgaccgcccg acgtcgaccg cctcgccgca ctgacggcac ttcccttttt
    10261 cgtcgacctc ctccgggtac ctccagccgt gcttggggca ctcggcggtc tgcatgcaga
    10321 ccatcccctg ggaaaggagg cgctcgaacg gctcgtttcc cggggtcaac cccttgtcgc
    10381 ggagatactt gtggaagaag cgggcgtaga tgaggtgcat gcaggcgtgc tccacccccc
    10441 ccacgtactg gtccacgggc gcgaatcggc gcatcttttc cgggtcgagc ggcgcgcggt
    10501 cgttcttcgg gtcgatgaac cgcaggaagt accaggagga ttcgacgaag gtgtcgaacg
    10561 tgtcggtctc tcgccggccg ggcttcccgc aggaggggca cggcacgcga agccaatgct
    10621 ccgcgccggc cagcgggttt ccccgctccc gcgtgtacgg aaggtcctcc ggaagcacca
    10681 ccggaaggtc cttctccggg acggggacga ttccgcacgc atcgcagtgg atcaccggga
    10741 tcggacaacc ccagtaccgc tgccggctga cgccccagtc gcgaagacgg tactggatcg
    10801 tcccgcggcc catccccttt ccttcaaggt gaagcgtgat cgccttcttc ccctcttcgc
    10861 tcgagatccc gtcgaacgcg ccggagccga ccaggcgtcc cggcccttcg tgcgccgcgg
    10921 tcatcgtcgc cggatcgagc gcctccccct cgggctgccc aacgaccgcc accgggaggc
    10981 catacttgcg ggcgaattcg aagtcgcgct ggtcgtgggc cggcaccgcc atcaccgcgc
    11041 cggtgccgta gtcgtacagg acgaagttgg cggcgtagac cggcatccgg ttccccgtga
    11101 cggggttcac gcaatacccc ccgatgaaca ctccttcctt cacgaggtcc tcgctcgtcc
    11161 gggcgatccg gtcctggctc gcgacccggt cgacgaactc ccgcacctcg cgctctctgc
    11221 cagtccgctt cgcgaattcc atcaccatcg ggtgctcggg ggccatgctc atgaacgtgg
    11281 cgccgaaaag cgtgtcgggc cgtgtcgtga acaccgtgac gtcgccgccc cccccgtcga
    11341 gggggaagcg gatctccgcc ccctcgctgc ggccgatcca gttgcgctgc atctcgagga
    11401 tgttcgccgg ccaccgtccc tcgagctcct tgtgcccgga gagcagctcc tcggcgtact
    11461 tcgtgatgcc gatgaaccac tggtcgagct ccttctgggc caccggcgtg tggtcgtgga
    11521 tgaagcacgt cccgtcgcga ttcacctgtt cgttggcgag cacggtctgg cactctccgc
    11581 accagttgag cgtggcgcgt ttgcgatacg cgaggccgtc ccggagcatc cacaggaaaa
    11641 agagctgctc ccatttgtaa tattccgggg agcaggtggc gaactcgcgg tcccagtcgt
    11701 aggagatccc catctccttc agctgctccc gcatgaaggc gatgttttcc cacgtccatt
    11761 tcgccgggtg cgtcccgtgc cggtgcgcgg cgttttcggc gggcagtccg aacgcgtccc
    11821 agccgatcgg gtgaagcacc tggaacccct gcatccgctt gaagcgcgcg agcaggtcgc
    11881 cgatcgtgta gacccgcacg tgccccatgt ggatccggcc ggaagggtac gggaacatct
    11941 cgaggcagta atatttcggg gcggacaact cgtccgggca ccgggacacg ccggcctcgt
    12001 tccacctctg ctgccacttc cgctcgatct cctgcggctt gtacttcacg ccgacccctc
    12061 cgccgttgcc gtattccgca cgatttcgga gatccggtcg aggatcccgt tgatgaacgc
    12121 gccggattcc tcggagccga accgtttgcc gagctcgacc gcctcgttga tcgccaccgc
    12181 gaacgggatg tcgctcccga gggagagttc gtacgcgccg aggcgcagga tgttccggtc
    12241 caccagcgtc atccttccga cccgccagtg ctccgccgcc tccccgatga gggcgtcgat
    12301 ctccccgatg cgcccccata cgccgcggac cgcctccgcg tagtagccgg cgtcggaagg
    12361 aggatccgag gccagcgcga aaagggggat ggcctcgtcc gggccaaccc cgagtgcgtc
    12421 catcatgaag agggtctgga aaaccttctc ccgggattcc ctgcgcacct attgcctcaa
    12481 ttcaatggtc cagaggcatt ggaccgccgg ccgctcccgc catccatggc tcccgcggca
    12541 cttgccc
//
LOCUS       DASVJL010000273         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1170284, whole genome shotgun sequence.
ACCESSION   DASVJL010000273 DASVJL010000000
VERSION     DASVJL010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170284"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1807
                     /locus_tag="VF357_05725"
     CDS             41..1807
                     /locus_tag="VF357_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX7523678.1"
                     /translation="MDNNKPEPADLPGETDPGESPVAGAPAGEEDFARMFAASLQSTG
                     EGQVIHGKVIKVLKDFVAVDINKKSEGMLPLEDIPEEERGALNPGDPIEVMTEGYDPS
                     LGAIRLSRSKVMKIRVWDDLQKAFDDGTPVHGAIVAKVKGGYTVDIGVKAFLPGSQVA
                     LRPVRDTDPVIGISGKFKILKFSRKKANVVVSRRAFMEEEREVQRSGLLDQIKEGDVV
                     EARVKNITDYGVFMDLGGLDGLMHVTDMSYGKVGHPSDLCKVGEVYQVRVIRFDRERG
                     RISLGLKQTKSDPWLDLDAKYQPGMRVHGKVTNTTKYGAFIEVEEGVEGLLHISEMSW
                     SKRLKDPSEVMKAGDAVEVVVLKVDKANKKISLGYRQLLSNPWDDLRARHPEGSIVEG
                     VVKNVADFGVFVDVGEDIDGLVHVSDLSWNTRVKNPSELFKRGDHVRAKVLKIDPESQ
                     KFSLGIKQLAEDPWAGIEKRFRKGDTITGKVTRMADFGAFVEIADGVEGLVHVSEISR
                     EKVESPAAVLKVGDVVGAIVLGVDRSNKKVSLSIRGHADELDRKNMESYLGKQADAPN
                     ENIGALGEALLAKFKANGEQNH"
     gene            1814..2713
                     /gene="sppA"
                     /locus_tag="VF357_05730"
     CDS             1814..2713
                     /gene="sppA"
                     /locus_tag="VF357_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX7523679.1"
                     /translation="MAETYLSAPPRRKPFLRGCLTVLLVLGGFFALLLIISHMDDLPL
                     TRGEKVAVISVSGLISDSEPTIEALKKFGKDDSVKAIVLRIESPGGGVGPSQEIYEEV
                     KKVRAKKPVLASMGALAASGGYYIACAAQRVYANPGTITGSIGVIMPFMNVKDLVEKI
                     GVKGMTVKSGVFKDMGSPIRDMTPQERELLQGVVDNVHLQFVNAVAAGRNLNREDVLR
                     IADGRIFTGEQAKELGLVDVLGNLEDAISDAGKLGKISGEPKVVTSAKKKISILELLR
                     EETRSLIDEKISGNHLRLNFLAQ"
BASE COUNT          599 a          855 c          957 g          442 t
ORIGIN      
        1 gtatagtaaa caattcggaa atcaaccaca ggagcgcgga atggacaaca acaaacccga
       61 accagctgac cttcccggag agacggaccc gggagaatcc cccgtcgccg gcgcaccggc
      121 cggggaagag gatttcgcac ggatgttcgc ggccagtctc cagtccaccg gggaagggca
      181 ggttatccac ggcaaggtca tcaaggtgct gaaggatttc gtcgcggtcg acatcaacaa
      241 gaaatccgag gggatgctgc cgctcgagga catcccggag gaggagcgag gcgcgctgaa
      301 cccgggcgac ccgatcgagg tgatgaccga ggggtacgac ccgtccctcg gtgccatccg
      361 cctctcccgt tcgaaggtga tgaagatccg ggtgtgggac gacctgcaga aggcgttcga
      421 cgacggcacg ccggtccacg gggcgatcgt cgccaaggtc aagggcggct acaccgtgga
      481 catcggtgtg aaggcgttcc tcccgggcag ccaggtggcc ctccggccgg tgcgggacac
      541 cgacccggtg atcggcatct ccgggaagtt caagatcctc aaattctccc gcaagaaggc
      601 gaacgtggtc gtctcccgcc gcgcgttcat ggaggaggag cgcgaggtcc agcgttccgg
      661 gctcctcgac cagatcaagg agggcgacgt cgtcgaggcg cgggtgaaaa acatcaccga
      721 ctacggcgtt ttcatggacc tcggggggct cgacggcctg atgcacgtga ccgacatgag
      781 ctacgggaaa gtggggcacc cttccgacct gtgcaaggtg ggcgaggtct accaggtgag
      841 ggttatccgc ttcgaccgcg aacgcggccg catctccctg ggcctcaagc agacgaaatc
      901 ggatccgtgg ctcgatctcg acgcgaagta ccagccgggg atgcgcgtcc acggaaaggt
      961 caccaacacc acgaaatacg gcgcgttcat cgaggtcgag gagggggtgg aagggctgct
     1021 ccacatctcc gagatgtcgt ggagcaagcg gctgaaggat ccgtccgagg tgatgaaggc
     1081 gggcgacgcg gtcgaggtgg tggtcctcaa ggtcgacaag gcgaacaaga agatctccct
     1141 cgggtacagg cagctcctct cgaacccgtg ggacgatctc cgcgcgcggc accccgaagg
     1201 ctcgatcgtc gagggcgtgg tgaaaaacgt ggccgacttc ggcgtcttcg tcgacgtcgg
     1261 ggaggacatc gacggcctgg tccacgtctc cgacctttcc tggaacacgc gggtgaagaa
     1321 cccgtccgag ctgttcaaga ggggggacca cgtccgggca aaggttctca agatcgaccc
     1381 cgagtcgcag aaattctccc tcggcatcaa gcagctcgcg gaggacccgt gggcggggat
     1441 cgagaagcgg ttccggaaag gcgacaccat cacggggaag gtgacccgca tggccgactt
     1501 cggggcgttc gtcgagatcg ccgacggagt cgaggggctg gtccacgttt cagagatctc
     1561 ccgggagaag gtcgaaagcc cggcggcggt gctcaaggtc ggcgacgtgg tgggcgcgat
     1621 cgtcctcggc gtggaccggt cgaacaagaa agtctccctc agcatccggg gacacgccga
     1681 cgagctcgat cggaagaaca tggaatcgta cctcgggaaa caggccgacg ccccgaacga
     1741 gaacatcggg gccctgggcg aagcgctcct ggccaagttc aaggccaacg gagagcagaa
     1801 ccactaaccc ccgatggctg aaacctacct ctccgcgcca ccgcgccgga aaccgttcct
     1861 gcgcgggtgt ctcaccgtgc tcctggtcct cggaggattc ttcgccctcc tgctgatcat
     1921 ctcgcatatg gacgacctcc ccctgacccg gggggaaaag gtggcggtca tttcggtctc
     1981 cggcctgatc tccgactccg agccgaccat cgaggcgttg aagaagttcg gcaaggacga
     2041 ctcggtcaag gcgatcgtcc tccggatcga atcccccggg ggcggggtgg gtccgtccca
     2101 ggagatctac gaggaagtga agaaggtgcg ggcgaaaaaa ccggtcctcg ccagcatggg
     2161 ggcgctggcg gcgtccggcg gctactacat cgcctgcgcg gcccagcggg tgtacgcgaa
     2221 ccccgggacg atcaccggct ccatcggcgt catcatgccg ttcatgaacg tgaaggacct
     2281 ggtggagaag atcggcgtga aggggatgac ggtgaaaagc ggcgtcttca aggacatggg
     2341 ttcccccatt cgcgatatga cgccccagga gcgcgaactt ctccaggggg tggtcgacaa
     2401 cgtccacctc cagttcgtca acgccgtggc ggccggccgg aacctcaacc gggaggatgt
     2461 cctccggatc gccgacgggc ggatcttcac cggggagcag gccaaggagc tggggctggt
     2521 cgacgtcctc gggaacctcg aggacgcgat ctccgatgcg gggaagctcg ggaagatctc
     2581 cggagagccg aaagtcgtga cctccgcgaa gaagaagatc tccatcctcg aactgctccg
     2641 ggaggagacg cgctcgctga tcgacgagaa gatatccgga aaccatctgc ggctgaattt
     2701 tctcgcccaa taaatctttc ggagggaccc ctggaatgac gaagagcgac ctggtggaga
     2761 agctgtccga gtcacttacg agcctgacca agaaagagtg cgaggtcatc gtcgacaccg
     2821 ttttcctcaa catgaaggac gccctccacc gcg
//
LOCUS       DASVJL010000274         5757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_981513, whole genome shotgun sequence.
ACCESSION   DASVJL010000274 DASVJL010000000
VERSION     DASVJL010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981513"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..945
                     /gene="rsmG"
                     /locus_tag="VF357_05735"
     CDS             283..945
                     /gene="rsmG"
                     /locus_tag="VF357_05735"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX7523680.1"
                     /translation="MVRSGGGGEVSPDLLREVLAEAGIPAFPGAASLLAAHANEMLRW
                     NRSIRLTAITDPVEVAVKHVVDSLLLLRFAPFPGRILDFGSGAGYPGIPLALYLPGAR
                     VTVLEASVKKCAFLSRARGMLRLSNVDVVQARAEERKRLPLPPHDHIVTRATAPAGGV
                     IPLLSPYLADGGRFLFMLGPGAREGVPGGLPGTVTRRERFHLPRGMGEREIVEIEPGG
                     KG"
     gene            950..1309
                     /locus_tag="VF357_05740"
     CDS             950..1309
                     /locus_tag="VF357_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523681.1"
                     /translation="MYERYYEPLISREEYLRRIARSGGLASAAVLAALFLGVSGYHWI
                     EGLPWVDSILNAAMILGGMGPVAELHTTAGKLFAAAYALFSGLMFIVVAGLLFAPVIH
                     RFLHKFHMDADRKDSDE"
     gene            1394..2176
                     /locus_tag="VF357_05745"
     CDS             1394..2176
                     /locus_tag="VF357_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7523682.1"
                     /translation="MARILTIANQKGGVGKTTTAVNLAASLSVMEKKTLLVDLDPQAN
                     ASSGVGGTPGGDEERNVYRVLMGEIPVAGAIRETGLPFLYLLPASADLIGAEIELVPM
                     ERRERRLAESLAPVAEEYEVIVIDCPPSLGLLTVNALCAADSVLIPLQCEYYALEGLS
                     SLFRTIDRIREEMNPGLRIEGVALTMFDARNNLAHQVADEAREILKDQVFQTVIPRNV
                     RLSESPSHGKPALLYAVSSRGAQSYLELAREMVNRWNGRTSP"
     gene            2152..3033
                     /locus_tag="VF357_05750"
     CDS             2152..3033
                     /locus_tag="VF357_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7523683.1"
                     /translation="MERKNEPVKKKVLGRGLSALLTGTTPSAGAPGAGRDPGFLQIPV
                     EKIRAGSLQPRRTFSPEALEELVASIREKGVLQPVLVRPTPDGYELVAGERRFRAAEA
                     AGLSTIPAVVRRLSDREALEAALVENIQRADLNAIELAEGYQRLVHDFSLSQEQVAER
                     VGKDRATVANTVRLLKLPSPVRQAVADGRLSAGHARALLSAPPEHASAICETVLRRGL
                     SVRETERLCGPAGKRKAARSAAPPDAHRKSLEEELSRRGGTRVRLRGTFMKGRVEISY
                     FSPEELDRLCDVLFRGR"
     gene            3556..4011
                     /locus_tag="VF357_05755"
     CDS             3556..4011
                     /locus_tag="VF357_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEX7523684.1"
                     /translation="MELVSKIFETLEISRLALLQLGLVVVLAFLLSTLLIRPILRIFE
                     ERENLSVKPVEESRRLLSEADEKARGYDEALRKGAAGALARKRRVMEETSRAGRRQVE
                     AVVEETNLKVEELKGRIAAEKESAAAFLRDQVSQLSREIARKVLGRPVA"
     gene            4008..4619
                     /locus_tag="VF357_05760"
     CDS             4008..4619
                     /locus_tag="VF357_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEX7523685.1"
                     /translation="MSRPRTLACRALRVAAPLAVSLCAAAVVFAAEESGAHPGGGVHV
                     PWGEILKQAINFLILASVLVYFLRKPVSSFLKERSELLRKTIDDAAKARAEAAEKLAA
                     IETRTAKLADEIAGMNAKMDVEAAAEAQRLQETVAVEISRIRAQSEFTGEQEVKKARE
                     ELRREASLLTAHAAEELVRKTLSPEDQERLVRENLEKIERIVH"
     gene            4616..5158
                     /gene="atpH"
                     /locus_tag="VF357_05765"
     CDS             4616..5158
                     /gene="atpH"
                     /locus_tag="VF357_05765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX7523686.1"
                     /translation="MIGGSLARRYARALRDIGQEDRQVRQVLSEVEEFARLLDLSPDL
                     REVLEAGHVNRRDKQSALGQVLSKAGFLPSTKSFLSLLVDKGRMNVLLPILGELRRMV
                     EELEGIERVGVSVPMPLSPPQKERLKEMLERRTGKKVVLEETVDPAVLGGLVVRMGST
                     VYDGSVRTQIRQIRENLQKG"
     gene            5169..>5757
                     /locus_tag="VF357_05770"
     CDS             5169..>5757
                     /locus_tag="VF357_05770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX7523687.1"
                     /translation="MSIRAEEITEILKSQIKGYEKRIDVAETGVVLSVGDGIARVHGL
                     EKAMAGELLDFPGDVRGMVLNLEEDNVGVVLLGEDRLIKEGDTVRRTGRIVEVPCGNA
                     MIGRVVNALGQPVDGRGPIETKDFRRVEIKAPGIVKRQPVKEPLQTGLKAIDAMIPIG
                     RGQRELIIGDRQTGKTAVATDTIINQKGAGVICIYV"
BASE COUNT         1000 a         1841 c         1951 g          965 t
ORIGIN      
        1 aggaagaacg gggggcactg cgtggggaac cccgatggcc atggacggcc aagtgccgcg
       61 ggagccatgg atggcgggag cggccccgct gcgccctgcg tccccgaacc ccggaggcgc
      121 atcaaagggg gtatcccgcg aagatctatg cgcctcctta tacggtaccg tatttgtgaa
      181 acgcgccact aagcgggaaa acgacgggat gatatccgta tctccttctc ggacaaggag
      241 ttttatggaa tgttccacgt ggaacatccc gggggggcgg ccatggtgag gagcggggga
      301 gggggggaag tctcccccga cctgttgcgg gaggtcctcg ccgaggcggg gatccctgcc
      361 ttccccgggg cggcttcgct gcttgcggcg catgccaacg agatgctccg gtggaaccgg
      421 tcgatccgcc tgactgccat caccgacccg gtagaagtgg cggtgaaaca tgtggtggat
      481 tctctcctgc tcctccggtt cgcgccgttc cctggtcgca tcctcgattt cggttccgga
      541 gccgggtatc ccggcattcc attggccctg tatctcccgg gggctcgcgt aaccgttctg
      601 gaggcctccg tcaaaaagtg cgcgttcctc tcccgggccc gggggatgct gcggctttcg
      661 aacgtcgacg tcgtccaggc gagggcggag gaacggaaga ggctccccct tcccccgcac
      721 gaccacatcg tcactcgcgc gacggcaccg gcgggcgggg tgatcccgct cctgtcgccg
      781 tacctcgccg acgggggacg cttcctgttc atgttggggc cgggggcgcg ggagggtgtt
      841 ccgggaggcc tcccgggcac ggtgacccgc agggaacgat ttcacctccc ccgcgggatg
      901 ggggagcggg agatcgtcga gatcgaaccc gggggaaagg ggtgaggaaa tgtacgagcg
      961 ctactatgag ccgctgattt cacgggagga atacttacgc cggatcgcca ggagcggggg
     1021 attggcctcc gccgcggttc tcgccgccct gtttctgggg gtgtccgggt accattggat
     1081 cgaggggctc ccttgggtgg actcgatcct caacgccgcg atgatcctcg gggggatggg
     1141 gccggtcgcg gagctgcata cgacggcggg aaaattgttc gccgccgctt acgcgctctt
     1201 cagcgggctg atgttcatcg tggtggccgg cctcctgttc gcccccgtca ttcatcgatt
     1261 cctccacaaa tttcatatgg atgccgaccg caaggacagc gacgaatgac cgcgcgcggg
     1321 atttccccct accgcccccc gcccccccgt ggtacaagat accgttgtgt ttccggaaaa
     1381 aggagggccc gttttggccc gtatcctcac catcgccaac cagaagggtg gtgtcgggaa
     1441 gaccaccacc gcggtcaacc tggccgcctc cctctccgtg atggagaaga agacgctgct
     1501 cgtcgacctc gacccgcagg ccaacgcttc ttcgggcgtg ggagggaccc ccgggggaga
     1561 cgaggagcgg aacgtctatc gcgtcctcat gggggagatc cccgtggcgg gggcgatccg
     1621 ggagaccggt cttcccttcc tctatttatt acccgcttcc gccgacctga tcggggccga
     1681 gatcgaactg gtgccgatgg agcggagaga gcggcgcctg gccgaatcgt tggctcccgt
     1741 tgcggaggag tacgaggtca tcgtgatcga ttgcccccct tccctggggc tgttgacggt
     1801 gaatgccctc tgtgccgccg attcggtact gattcctctt cagtgtgaat attatgcatt
     1861 ggaagggctt tccagcctct tccgcaccat cgaccggatc cgggaggaga tgaaccccgg
     1921 gctgcggatc gagggggtgg cgcttaccat gttcgacgct cggaacaacc tggcgcacca
     1981 agtcgcggac gaggcacgcg aaatactaaa agatcaagta tttcaaacag ttataccaag
     2041 gaatgtccgc ctgtccgagt cgccctccca tggaaagccg gcgcttctgt acgccgtttc
     2101 ctcccgcggg gcgcaaagtt atctcgaact ggcccgggag atggtgaatc gatggaacgg
     2161 aagaacgagc ccgtaaagaa gaaggttctg ggccggggcc tctccgccct gttgacgggc
     2221 accaccccct ccgcaggggc tcccggcgcc ggacgcgacc ccgggttcct ccagatcccc
     2281 gtggaaaaaa tccgtgcggg aagtttgcag ccccggcgaa ccttctcccc ggaagcgctc
     2341 gaggaactcg tcgcctcgat ccgggagaaa ggggttctgc agccggtgct ggtccggccg
     2401 accccggacg ggtacgagct ggtcgccggc gagcggcggt ttcgcgccgc ggaggccgcg
     2461 gggctttcca ccatcccggc ggttgtgcgc aggctctccg accgtgaggc gctcgaagcc
     2521 gccttggtgg aaaatatcca gcgggcggat ctgaacgcga tcgagcttgc cgaggggtac
     2581 cagcggctgg tgcacgactt ctccctctcc caggagcagg tggccgagcg ggtggggaag
     2641 gaccgcgcga cggtggccaa caccgtgcgg ctcctgaagc tgccatcccc ggtccgccag
     2701 gcggtggcgg acggacgcct ctccgccgga cacgcccgcg cgctcctctc cgcgccgccg
     2761 gagcacgcgt cggcgatctg cgagaccgtg ctgcgccgcg gcctctcggt tcgggagacg
     2821 gagcggctgt gcggccctgc ggggaaacgg aaagccgcgc ggagcgcggc cccgccggat
     2881 gcgcaccgga aaagcctcga ggaggaactc tcccgccgcg gggggacgcg ggtgcggctg
     2941 cgcggtacgt tcatgaaggg acgcgtcgag atctcctatt tctcgccgga ggagctcgat
     3001 cgtctctgcg acgtcctgtt caggggccgt tgaccaaaaa ataagtattg caacccggtt
     3061 tgcctatgct atattgcgcg tcatacgcta ccatgcacta tccgcgaata cggataaatt
     3121 ccgagggaaa ggagtgatga ccaatgcgca agtatctgtc aatgctgttc gtgctgatgc
     3181 tggccgcgac gatcggcctc tccgcttgtg cgaagaagga agagccgcct ccgccgccgc
     3241 ctccgccgcc ggctgccgca cctgccgaca acatggcgcc tggagcgcct ggagcgcctg
     3301 gagccccagg agcacctgga gcgccgggag cacctggagc accgggagca ccgactgaaa
     3361 tgcccaagaa gtagtttccc gggcagtacg gttccaggaa gggcgccggg gtatccccgg
     3421 cgcccttcct catttgccgc ctgccgaccg tcaagtagcc cttgacagtt ccgcggcggg
     3481 cgtggtacca atcttccgtt tctcttgtag ttgtccttcc gtagaccagt ccgcccccgg
     3541 gggagtgccg gtttcatgga acttgtgagc aagattttcg agacgttgga gatctcccgg
     3601 ctcgccctgc tccaactggg cctggtggtc gttctcgcgt tcctcctttc caccctcctc
     3661 atccgtccga tcctccgcat cttcgaggag cgggagaacc tctcggtgaa gccggtcgag
     3721 gaatcccgcc ggctcctgtc cgaggccgac gagaaggccc gtggatacga cgaggctctc
     3781 cgcaagggcg cggccggggc gctcgcccgg aagcgtcggg tcatggaaga gacttcccgt
     3841 gccggccgca ggcaggtcga agccgtggtc gaggagacga acctgaaggt cgaggaactg
     3901 aagggccgga tcgccgccga gaaggaatcg gccgccgcgt tcctgcgaga ccaggtgtcg
     3961 cagctctcca gggagatcgc ccggaaagtc ctcgggaggc cggtcgcgtg agccgcccgc
     4021 gaaccctcgc ctgccgcgca ttgcgcgtcg ccgcgcccct tgccgtgtcc ttgtgcgccg
     4081 ccgccgtcgt gttcgccgcg gaggagagcg gggctcaccc agggggcggc gtccacgtcc
     4141 cctgggggga gatcctcaag caggcgatca acttcctcat tctcgccagt gtgctcgtct
     4201 acttcctgcg caagccggtc tcctcctttc tcaaggaacg aagcgaactg ctgcgcaaga
     4261 cgatcgacga tgccgcgaaa gcccgcgcgg aggcggcgga gaagctggcc gccatcgaaa
     4321 cccgtaccgc gaaactggcc gacgagatcg ccgggatgaa cgcgaagatg gacgtcgagg
     4381 ctgccgccga ggcgcagcgc ctgcaggaga ccgtcgccgt cgagatctcc cggatccgcg
     4441 cccagtcgga gttcaccggc gagcaggaag tgaaaaaggc ccgcgaggag ctgcgccggg
     4501 aggcgtccct ccttacggcc cacgccgccg aggaactcgt ccgcaagacc ttgtcgcccg
     4561 aggaccagga gcgtctggtc cgtgagaacc tcgaaaagat cgagagaatc gtccattgat
     4621 cggcggaagc ctcgccaggc ggtacgcccg cgcgctgcgg gacatcggcc aggaggaccg
     4681 gcaggtccgc caggtgctct cggaagtcga ggagttcgcg cgccttctcg atctgtcccc
     4741 cgatctgcgc gaggtgctcg aggccgggca cgtcaaccgc agggacaagc agagcgccct
     4801 cggccaggtc ctctccaagg ccgggtttct cccctccacg aaatcgttcc tttcccttct
     4861 ggtcgacaag gggcggatga acgtgctcct tccgatcctc ggcgaattgc ggcgcatggt
     4921 ggaggagctc gaggggatcg aacgggtcgg cgtctccgtc cccatgccgt tgtctccccc
     4981 gcagaaggag aggctgaagg agatgctgga gcgccggacg gggaagaagg ttgttctgga
     5041 ggagaccgtg gatcccgcgg tgctcggggg gctcgtcgtc cggatgggtt cgaccgtgta
     5101 cgacggcagc gtgcgcacgc agatccgcca aatccgggaa aatttacaaa aggggtgagg
     5161 cagtcgccat gagcatccgc gcggaagaga ttaccgagat cctgaaaagt cagatcaagg
     5221 ggtacgagaa gcggatcgac gtcgccgaaa ccggcgtcgt cctctccgtc ggcgacggga
     5281 tcgcccgcgt ccacggcctc gaaaaggcga tggcgggcga gctcctcgat ttcccgggcg
     5341 acgtccgcgg gatggtgctc aacctcgagg aggacaacgt cggcgtggtc ctcctcggcg
     5401 aagaccgcct gatcaaggaa ggggacacgg tccgtcggac cggacgaatc gtcgaggtgc
     5461 cgtgcggcaa cgcgatgatc ggccgcgtcg tcaacgccct cggccaaccg gtggacggcc
     5521 ggggcccgat cgaaacgaag gatttccggc gcgtcgagat caaggcgccc gggatcgtca
     5581 agcggcagcc cgtgaaggag ccgctccaga ccggcctcaa ggccatcgac gcgatgatcc
     5641 ccatcggccg cgggcagcgc gagctgatca tcggcgaccg ccagaccggg aagaccgcgg
     5701 tggccaccga cacgatcatc aaccagaagg gcgccggggt catctgcatc tacgtcg
//
LOCUS       DASVJL010000275         5190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_605955, whole genome shotgun sequence.
ACCESSION   DASVJL010000275 DASVJL010000000
VERSION     DASVJL010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605955"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="VF357_05775"
     CDS             complement(<1..1457)
                     /locus_tag="VF357_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX7523688.1"
                     /translation="MFLGIDVGSISMKFSLFLPEGSTDLSADLRGTFLDPEAIALPGA
                     GKGYNLSYDRLLGDPVRKVPERLRRWFAVLGEENVRAIAVTGRSGRQLAPLIGATYEN
                     DFRCLVKAITVTHPEVRTIFEMGGENAKVIRIEASGDAGLLTVKDYDTNGDCAAGTGS
                     FIDQQAHRMKLQVEQVGEMVAHAASAARIAGRCSVFAKSDMIHAQQKGYSPEEILKGL
                     CDAVARNFKSSINKGKDPVPKIALVGGLFENSGVVRAIRDAFGFSAEDVVVPRGFAHM
                     GALGAAAASSERAGKEASTIPGAPAVEEPGERTFPSWPPLTTENVVFLRDRVQAPPKP
                     EGRPEVFLGIDIGSVSTNFAVTDWEGNLLKEIYVGTQGRPVQVVTDGLQALREEFGDT
                     ISIRGVGTTGSGRELIGELVGADTVQDEITAHKTGSTFISKRFFDASVDTIFEIGGQD
                     SKFIGLENGVVTDFAMNDACAAGTGSFLEEQAERLG"
     gene            1694..2122
                     /gene="tatA"
                     /locus_tag="VF357_05780"
     CDS             1694..2122
                     /gene="tatA"
                     /locus_tag="VF357_05780"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX7523689.1"
                     /translation="MFGIGFQEMVIILVVALIFFGPKRLPDLAKSLGKGIAEFKKASE
                     EVRKGIEDAVKEESAAETPKPPEDLSSYGKAPGSAPVPEEPAKPVSPPPGMILTPASG
                     TPPDHPPVEPAPSTGDPATADSAAPGTATGETPSTPPRQG"
     gene            2129..2887
                     /gene="tatC"
                     /locus_tag="VF357_05785"
     CDS             2129..2887
                     /gene="tatC"
                     /locus_tag="VF357_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX7523690.1"
                     /translation="MSTNAGEIRQPLTEHLDELRRRLIRALIALGIGTALCYNFAERI
                     YSALLSPLTAALPADSHLIFTELTEAFLTYFKLSLWGGFILTSPVIFYQAWRFVSPGL
                     YLKERKLFLFFAAWSTFGFLAGMAFAYFVAIPAILSFFLSFGRSVVVPMPSMRESLSI
                     VLRLLMIFGVMFELPLVLFLAGRGGILTAEFLRKWRKGAVLGAFLLAAVLTPPDAVSQ
                     IMIAFPLYALFEIGIVLCAIGGRRRASALPGSPA"
     gene            2961..4433
                     /locus_tag="VF357_05790"
     CDS             2961..4433
                     /locus_tag="VF357_05790"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HEX7523691.1"
                     /translation="MIIECQTCHARFRLDESRIKGRGARVKCRKCGDSIVVLKDGAPA
                     TPAPGGDGFFDLGSAVRDSAGESPGAPPPVGNLIPFPGQARPAVPFAAESSSPALEAA
                     EPGRDEVDLAFDRVLSAGAETSSPSIREAEIPAPAEADVPVTHDVPAGHGPRPELDLG
                     ALTLDLGPEEKLDLPPAEELEPPLVEPPAVEPSSEFQGERGFLISDSDTLDFLQEKHR
                     DAESEAPPGVSDISTEITSGPVIVGSSFLREPDASPTPREDRASSTSEGISLEGNVTP
                     PAAPVIEVPSYREEESTPAPPVESAVRRGSPVPESPRPRFSVGLAVAVVLAVLLAAGG
                     YLGLTTSGRKMFERAIRGVTALRGGVPAAPAAPKYDLRNVIGYYESGSASPKILVIKG
                     QVANLSTVEKSGIRVHATLLDNADTVLAQQAVYAGNMLSGEAIRKGNRDALLKTLGNR
                     LGEGLVNMNVAAGKAIPFMVVFFDAPPTMDSYKLEANDGE"
     gene            complement(4469..4654)
                     /locus_tag="VF357_05795"
     CDS             complement(4469..4654)
                     /locus_tag="VF357_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX7523692.1"
                     /translation="MFGLGLPELLIILVIVVLLFGAGRLPQIGAGIGEGIRNFKKSMK
                     EKDEVDVTPTKGDGDKK"
     gene            complement(4727..>5190)
                     /locus_tag="VF357_05800"
     CDS             complement(4727..>5190)
                     /locus_tag="VF357_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HEX7523693.1"
                     /translation="DGSRGMLVGNGSAGMFLVCAENVPNPYVLPRPFRVNAGAVHSYC
                     RVPGGRTAYLSELVAGSGVLLVDDSGGGEAAWVGRAKVERRPLVLVRAVSPPGNEHSI
                     VLQNAETIRLVGPGGATPSIARIAAGDEVLLMEERAGRHFGVAVEETIREK"
BASE COUNT          850 a         1793 c         1647 g          900 t
ORIGIN      
        1 ccgagccgct ccgcctgctc ctcgaggaac gacccggtcc cggcggcgca cgcgtcgttc
       61 atcgcgaaat cggtgaccac gccgttttcg aggccgatga acttggagtc ctgcccgccg
      121 atctcgaaga tcgtgtcgac cgacgcgtcg aaaaagcgct tgctgatgaa ggtggagccg
      181 gtcttgtggg ccgtgatctc gtcctgaacc gtgtcggcgc cgaccagctc gccgatcagc
      241 tcccggccgg acccggtggt ccccacgccg cggatcgaga tcgtgtcccc gaactcttcc
      301 cggagtgcct gcagtccgtc ggtcaccacc tgcaccggac gcccctgcgt ccctacgtag
      361 atctccttga ggaggttccc ttcccaatcc gtcaccgcga agttcgtgga gaccgacccg
      421 atgtcgatgc cgaggaagac ctcgggccgg ccctcgggct tcgggggcgc ctggacgcgg
      481 tcgcgcagga agacgacgtt ttccgtcgtg aggggcggcc atgaggggaa cgtgcgttct
      541 cccggctcct cgacggccgg tgcccccggg atcgtcgacg cctcttttcc cgcacgctcc
      601 gaggaggccg ccgccgcgcc gagggcgccc atgtgggcga agccgcgcgg caccacgaca
      661 tcctccgccg aaaagccgaa ggcgtcgcga atcgcgcgga caactccgga gttctcgaac
      721 agaccgccga ccagggcgat cttcgggacg ggatccttcc ccttgttgat gctgctcttg
      781 aagttgcgcg cgaccgcgtc gcacagtccc ttgagaatct cctccgggga gtaccccttc
      841 tgctgggcgt ggatcatgtc gctcttggcg aacacggagc atcggccggc gatcctcgcc
      901 gcgctcgcgg cgtgggcgac catctcgccg acctgttcca cctgcagctt catccggtgg
      961 gcctgctggt cgatgaacga ccccgtaccc gccgcgcagt cgccgttcgt gtcgtaatcc
     1021 ttgacggtga ggagacccgc gtcgccggac gcctcgatgc ggatcacctt ggcgttctcc
     1081 ccgcccatct cgaagatcgt gcggacctcg ggatgcgtga cggtgatggc cttgacgagg
     1141 cagcggaagt cgttctcgta cgtcgccccg atgagcggag caagctgacg gccactgcgc
     1201 ccggtaaccg cgatcgcccg cacgttctcc tcgccgagga ccgcgaacca ccggcgcagc
     1261 ctctccggca ccttccgcac aggatcgccg aggagacggt cgtacgaaag gttgtacccc
     1321 ttcccggcac cggggagcgc aatcgcctcc ggatcgagga acgttccgcg cagatccgcg
     1381 gaaaggtccg tcgaaccttc cggcagaaac agggaaaact tcatgctgat cgagccgacg
     1441 tcgatgccaa ggaacaagtc gtctctcctc cctcgggaaa tatgggccca acgtgcactt
     1501 cgcatacaat ggtcgtgccg gtgcggcgat cgctgacaaa tcgcatggtt acgaaactcc
     1561 cgacacagcg tcgcgcccac agttctgcca gatcggataa ccgcttatcc gaacggacat
     1621 cttgcgactg tccttggaat tccagggcga ggcggggtat agtagtaagg aaagaaaacc
     1681 gaaaggggag cgaatgttcg gtatcggctt ccaggagatg gtcatcatcc tggtcgtggc
     1741 cctgatcttc ttcggtccga agcgcctccc ggacctcgcc aagagcctgg gcaaggggat
     1801 cgcggagttc aaaaaggcgt ccgaggaggt ccgcaagggg atcgaggacg cggtgaagga
     1861 agaatcggcg gccgagacac cgaagccgcc ggaggacctt tcctcgtacg ggaaggcccc
     1921 ggggagcgct cccgtgccag aggagcccgc aaagcccgta tccccgccgc cgggcatgat
     1981 tctcacgccc gcaagcggca ctcctccgga ccatcccccg gtggaacccg ctccatcgac
     2041 gggtgatccc gccaccgcgg atagcgccgc gccgggtacc gccaccggag agactccctc
     2101 cacacccccc cggcaggggt gacgccggtt gtcgacgaac gccggggaaa tccgccaacc
     2161 cctcaccgag catctcgacg aactccgacg ccgcctcatc agggcgctga tcgccctcgg
     2221 gatcggcacc gccctctgct acaatttcgc ggagcggatc tactccgcgc ttctttctcc
     2281 cctcaccgcc gccctccctg cggattcgca cctgatcttc accgaactga cggaggcgtt
     2341 cctcacgtac ttcaagttgt ccctgtgggg ggggttcatc ctcacgtccc cggtcatctt
     2401 ctaccaggcg tggcggttcg tgagccccgg gctgtacctg aaggagcgga agctttttct
     2461 ctttttcgcg gcatggtcca ccttcggatt cctcgcgggg atggcgttcg cctacttcgt
     2521 ggcgatcccg gcgatcctct ccttcttcct gtcgttcgga cggtcggtcg tggtcccgat
     2581 gccgtcgatg cgggaatcgc tctccatcgt cctgcgtctc ctgatgatct tcggagtcat
     2641 gttcgaactt ccgctggtgc tcttcctggc gggacgcggc gggatcctga cggcggagtt
     2701 cctgcgaaaa tggcgcaagg gggccgtcct cggggcgttc ctcctggccg ccgtgctgac
     2761 tccgccggac gcggtgtccc agatcatgat cgccttcccg ctgtacgcgc tgttcgagat
     2821 cgggatcgtg ctttgcgcca tcgggggacg ccggcgtgcc tcggccctcc ccggatctcc
     2881 ggcttgagtc cctcggggaa attgcctata gttcggaaaa aggcgcgacg tcgcgcaaca
     2941 ccgtcttccg gaggcccggg atgatcatcg agtgccagac atgccacgcg cggttccgcc
     3001 tcgacgagtc caggatcaag ggaaggggcg cccgcgtgaa gtgccggaaa tgcggggaca
     3061 gcatcgtcgt cctaaaggac ggcgctcccg cgactccggc gcctggcggg gacggtttct
     3121 tcgacctcgg ctccgcggtg cgggattccg ccggcgagag tcccggcgcc ccgcccccgg
     3181 tcgggaacct gatccccttc cctgggcagg cccggccggc ggtacccttc gctgcggaat
     3241 cctcttcccc cgccctggaa gcagcggagc cgggtaggga cgaggtggat ctggccttcg
     3301 accgggtcct ctccgccggc gcggaaacat cctctccgtc catccgggaa gcggagattc
     3361 ccgcccccgc ggaggcggac gttccggtga cgcacgacgt tcccgcaggc cacggacccc
     3421 gtccggagct ggacctcgga gcgttgaccc tcgacctcgg gcccgaggaa aaactggacc
     3481 ttcctcccgc ggaggagttg gagccgccgc tcgtagaacc gccagccgtc gagccgtcct
     3541 cggagttcca gggcgagcgg gggttcctga tcagcgattc ggataccctg gatttcctgc
     3601 aggagaaaca tcgcgatgcg gagtcggagg ctcccccggg ggtcagcgac atctccacgg
     3661 agatcacgtc cgggccggtc atcgtgggga gttcttttct gcgtgaaccc gacgcgtccc
     3721 cgacgccgcg cgaggatcgg gcttcttcga cctcggaggg gatctctctg gaggggaatg
     3781 tcaccccgcc ggcggcaccc gtcatcgaag tcccgtcgta ccgggaagag gaatccaccc
     3841 ccgccccccc cgtggagtcc gcggtgcgcc gtggatcccc ggtaccggaa tcccctcgcc
     3901 cacggttttc cgtcggactc gccgtggcgg tagtcctggc ggtcctcctc gcggccggtg
     3961 gctatctcgg cctcacgaca tcggggagaa aaatgttcga aagagcgatc cggggtgtca
     4021 ccgcgctacg ggggggggtc ccggcggcgc ctgcggcgcc gaagtacgac ctgcggaacg
     4081 tgatcggtta ttacgagagc ggcagcgcct ccccgaagat cctggtgatc aagggtcagg
     4141 tggccaatct ctccacggtg gagaagagcg gcatccgggt ccacgccacg ctgttggaca
     4201 acgccgacac cgtcctggcg cagcaggcgg tctacgcggg aaacatgctg tccggagaag
     4261 cgatccggaa ggggaaccgg gacgccttgt tgaagaccct ggggaaccgg ttgggggaag
     4321 gtttggtgaa catgaacgtc gcagccggca aggccatccc gttcatggtg gtgttcttcg
     4381 acgccccgcc gaccatggac agctacaagc tggaggcgaa cgacggcgaa taatcaccgg
     4441 cccggggcgg aaacccgccc ccgccgagct atttcttgtc gccgtctccc ttggtcggag
     4501 tgacgtccac ctcgtccttt tctttcatcg acttcttgaa attccggatc ccttccccga
     4561 tcccggcgcc gatctgcgga agccgaccgg ctccgaacag cagcaccacg atcacgagga
     4621 tgatgagaag ttccggaaga ccgagtccga acatgccccc tccttcgaat aacacgcctt
     4681 ggctcgaact tgttccagtc tactccggat cgcctcgggt tgtccgctac ttttcgcgga
     4741 tcgtctcctc gaccgccacc ccgaagtgcc ggcccgcgcg ctcctccatg agcaggacct
     4801 cgtccccggc cgcgatccgg gcgatcgacg gcgtggcgcc tcccggtccc accagacgga
     4861 tcgtctcggc gttctgcagc acgatcgagt gctcgttccc gggggggctg acggcgcgga
     4921 ccagcacgag cgggcggcgc tccaccttcg cccggccgac ccacgccgcc tctccccccc
     4981 cggaatcgtc gacgaggagc actcccgaac cggcgaccag ctcggaaaga taggcggtcc
     5041 ggcctccggg gacgcggcag taagagtgga ccgcgcccgc gttgacgcgg aacggacgtg
     5101 gaaggacata cgggttcggg acgttctcgg cgcatacgag gaacatcccc gcgctcccgt
     5161 tgccgaccag catcccgcgg ctcccatcga
//
LOCUS       DASVJL010000276         7393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1827379, whole genome shotgun sequence.
ACCESSION   DASVJL010000276 DASVJL010000000
VERSION     DASVJL010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827379"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VF357_05805"
     CDS             <1..343
                     /locus_tag="VF357_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523694.1"
                     /translation="TAISFCGFEERPLLAEIERLMSKHLTVVTDHPFASPARPAPPTV
                     FHTGRTPSKPRPFMISVDAAFLSAGRPVPSSPTERVSRHTGNEARPTSGPGGRKSGHR
                     DQFTSRGRSQR"
     gene            531..1148
                     /locus_tag="VF357_05810"
     CDS             531..1148
                     /locus_tag="VF357_05810"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7523695.1"
                     /translation="MDRGRLIAWIHSLGAWGFAGFILLQVIQVVAAPIPGEVTVVLGG
                     YLYGPVVGVVLSTIGLTIGSFLAFILSRFFGRPLTEKFVDAKTMERFDYLLHHKGAFL
                     VFLLFLIPGFPKDYLCYILGLGHLTTLEFLSIATTGRLLGTVLLTLGGTFLRNHQYYR
                     FFLLSGAAVVVVFLTIAYRDRLEQFFQRLHERHLRKKGKRPGEPT"
     gene            complement(1141..1734)
                     /locus_tag="VF357_05815"
     CDS             complement(1141..1734)
                     /locus_tag="VF357_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523696.1"
                     /translation="MLGAIFGGGEQLSYLEFEPGHSDIPAAEEGKLGNLAKALHDRPA
                     LKLEIEGHVDMEKDREGMRQQFFRRKVAAQKLADHASTGRPGPPLDNVRVETAEYPKY
                     LARAYTVEKFPKPRNFIGMSKDLPVPEMEKLMLTHIQVTDDNLRQLAVERASHVRDRL
                     VATEKVEPERVFLVEPKTLPPERKEKLRDSRVDFRIK"
     gene            complement(2068..3405)
                     /locus_tag="VF357_05820"
     CDS             complement(2068..3405)
                     /locus_tag="VF357_05820"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX7523697.1"
                     /translation="MEKGGKNAPKEEPGAKVQKYAVDSIRLAGGKVRFADASRAIPFV
                     TKLADIRVDVNGLGTEVGKKAATLVSFSTEAGETAELKGNLSLSPLGSEGTISLAKVV
                     LKKYAPYFSNEVRFDINGGTLDVRSGYSFTQGDSGPEFRLSGLGASVSDLRLRQREEK
                     EEFLVIPRFDVKEAEIDLVKKEITIGGIATAKGWIAVRRSSGGESNVAHLVPDDGLSA
                     KPTGAIGAQTKSDGERPAEKPWGITIKETVVDRYSVRFEDRTTDPPVEITLDRLRLKA
                     ENVATGGKQRGKFSFATLYNRKGSISLGGTFSVDPPSMNAKLQAKALPIGPLQPYYTE
                     KLKILLTGGAISAEGNVSFVASKGKPVRAGFRGEVSVKDFSSLDKALEEEFLTFAGLH
                     FGGVEVGYNLTSVAIREISLTDFYSRIIVHPDGTLNVQGIVGKERVGDRTTRH"
     gene            complement(3427..4455)
                     /locus_tag="VF357_05825"
     CDS             complement(3427..4455)
                     /locus_tag="VF357_05825"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX7523698.1"
                     /translation="MKWVKHIRSKPMVRKGLIAGVAALLVYALFGFLALPPILKSVLS
                     KSLTETLHRKAMLRDIRVNPFELSVSVRGLAISERDSPGTWVSVEEVFANLQLASVMR
                     GGPVLSEIRLSRPYVNIVRRPDGSYNFTDLIDQFIKKPAKESKSLKYSFNNIQIIAGS
                     MDFDDRPKKTRHEIRGIRIGVPFISNLRYYVDRYVQPSFAAVVNGEPIALKGKTKPFS
                     ESLETTFDVNISDLDIPHYLEYVPLQREYEILSAFLDVKAVISFTQHKNKPPTLSAEG
                     DVTLKEVRVSGKDKSPMIRLPMVKAVVSPSDLAARDFHLASLQVRDPEIDISLDRSGK
                     LNLLSLVP"
     gene            complement(4523..5539)
                     /locus_tag="VF357_05830"
     CDS             complement(4523..5539)
                     /locus_tag="VF357_05830"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX7523699.1"
                     /translation="MNLGELESVARSLVVPGKGILAADESAPTIEKRFKAIDFPSTEE
                     NRRAYRDLLFTTPRVSEFISGVILYDETIRQCSADGTPFTKVLERQGIVPGIKVDEGA
                     KALIGFPGEKITEGLDGLRGRLPEYRALGARFAKWRAVIDIGAGIPTRYCIQANAHAL
                     ARYAALCQEAGLVPIVEPEVLMDGGHTIERCEEVTTEVLSLVYAELTAHRVALEGTLL
                     KPNMVVPGKGCPAQAQAAQVAEATVRTLSRVVPPAVPGIVFLSGGQAPRQATEHLSAM
                     NAMGKHPWELSFSYGRALQDPVLKAWKGKAANVPAAKEAFHLRAKLNGAARHGNYSPA
                     MEAV"
     gene            5657..6019
                     /locus_tag="VF357_05835"
     CDS             5657..6019
                     /locus_tag="VF357_05835"
                     /inference="COORDINATES: protein motif:HMM:NF005297.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HEX7523700.1"
                     /translation="MAERRDDPVLVYSTERGLVCPKCRLPMAKCRCAKEEPAPAGNGI
                     VRVRRETKNRGGKTVTTVSGVPLGGEALRNLASDLKRRCGSGGTAKDGVIEIQGDHRE
                     TIVEELLHRGFTAKLAGG"
     gene            6127..>7393
                     /locus_tag="VF357_05840"
     CDS             6127..>7393
                     /locus_tag="VF357_05840"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7523701.1"
                     /translation="MKVLLLYPEFPDTFWSFRHALPFIGKRSAYPPLGLLTVSAMLPP
                     HWERKLVDLNVEELRDKDIAWADVAFLSAMLVQGSSLAQLIARCREAGLRTVVGGPVT
                     SADNPSYEGADHIVRGEAEGIIEEIVSDLEAGNARRHYEAVGRADMTVVPPPDLRLAR
                     LRRYSAMSIQYSRGCPFSCEFCDVIELFGRIPRTKTVEQVLEEFELLYRMGWRGSVFV
                     VDDNFVGNRQAIKDLLPRIADWMRSRGNPFSLFTQASINLAEDDELLSLMRTARFNKV
                     FIGIETPSAECNRAAGKMQNVKADLLACVRHIQEHGMEVMGGFIVGFDQDPPEIFERQ
                     IAFIREAAIPISMVGVLIALPNTRLWRRLSEEGRILRQTMGNNTEALLNFIPRMNPEA
                     LLAGYRKVLASIYSPSEYFERVQVMISRLG"
BASE COUNT         1290 a         2390 c         2193 g         1520 t
ORIGIN      
        1 taccgcgatc tccttctgcg gcttcgagga gcgcccgctg ctcgccgaga tcgagcgcct
       61 gatgagcaag cacctgaccg tggtcacgga ccaccccttc gcctctcctg cgaggccggc
      121 gcccccgacg gttttccaca cgggccggac gccgtcgaaa cctcggccgt tcatgatctc
      181 cgtggacgcc gcgttcctct ccgcggggcg acccgtcccc tcttctccca cggagcgggt
      241 gtcccgtcac accgggaacg aggcgcgccc gacctccggc cccggcggcc ggaagagcgg
      301 gcaccgggac cagttcactt cccggggccg ctcccagagg taaccacaga accgcaatcc
      361 ccgatcggac cgcccccgcg gacgcgtttc gtgtatgatc ttcgcaacgc caaccggggg
      421 ccggacctct gcagcaaaaa ctgaaattcc tcgtcaagcc gctcgttttt ctcttcgcct
      481 tcctcgctct cagtttctcc ctgtaccatc tcggcctctt ccactttttc atggaccggg
      541 gacgcctgat tgcctggatc cactccctcg gcgcatgggg atttgccgga ttcatcctgc
      601 tgcaggtgat ccaggtggtc gccgctccca ttcccgggga agttacggtg gtcctgggag
      661 ggtacctcta cgggccggtc gtgggagttg tgctctccac catcggattg acgatcggat
      721 cgttcctcgc cttcatcctc tctcgtttct tcggtcgccc cctgaccgag aagttcgtcg
      781 acgcgaagac gatggagcgg ttcgactacc tgctccacca caaaggtgcg tttctcgtct
      841 tcctgctgtt cctgattccc gggttcccga aagattacct ctgctacatc cttgggctgg
      901 gccatctcac cacgctggag ttcctctcca tcgccacaac ggggcgcctc ctcgggaccg
      961 tgctgctcac gctcggggga acgttcctga ggaaccacca gtattaccgt ttcttcctgc
     1021 tcagcggcgc ggcggtggtc gtcgtcttcc tgaccatcgc ctaccgggat cggctcgagc
     1081 agttcttcca acgacttcac gaacggcatc ttcggaagaa ggggaagcga cccggagagc
     1141 ctacttgatc cggaagtcga cccggctgtc ccgcagcttt tcttttctct ccggagggag
     1201 ggttttcggc tctacgagga agacccgctc gggctccacc ttctccgtcg cgacgaggcg
     1261 gtctctcacg tgggaggcgc gctccacggc gagctgccgg aggttgtcgt ccgtcacttg
     1321 gatgtgcgtc agcatcagct tctccatctc gggaacgggc aggtccttgg acatgccgat
     1381 gaagttgcga ggcttcggga atttctccac cgtataggcc cgggccaggt acttcggata
     1441 ctccgccgtc tcaacgcgca cgttgtccag gggcggtccc ggccgacccg tcgacgcatg
     1501 gtccgccagt ttctgcgcgg cgaccttccg ccggaagaac tgctgccgca ttccctcccg
     1561 gtccttctcc atgtccacgt ggccctcgat ctcgagcttc agggccggcc gatcgtgaag
     1621 agccttcgca aggttgccga gcttcccttc ctccgccgcc gggatgtcgg aatgtcccgg
     1681 ttcgaactcc agatacgaaa gttgctcccc gccgccgaag atggcgccca acaacgcaaa
     1741 aggcgaggtg gcggccttgg caaggaggtt cccgatgatc ttccagacga ctccccagat
     1801 gctgaatttc gggtcgtcga ggcgccgagt tctccagctt tccgcgcagg ccgatgtccg
     1861 cgagccggct ctcctctgag gagaggccgg agacgcgtcc tccgatctcc acgaggctgg
     1921 cagagtagtt cggcttgatg tacttgtcgc tgaagttcac cttgccgccc tgcagcgtca
     1981 ccgtgtcgat ccggacggga acctcgggcg cctgcgacgc gttgtccgcg gaagtcgcgt
     2041 ttacgcgtcc agccgccggc gccggtttca atgccgcgtt gtccggtccc ccactctctc
     2101 tttcccaacg attccctgga cattcagcgt gccgtccggg tgtactatga tccgggagta
     2161 gaagtccgtc agggagatct cccggatggc gacgctcgtc aggttgtacc ccacctccac
     2221 gccgccgaaa tggaggccgg cgaacgtcag gaactcctcc tcgagggcct tgtccaggga
     2281 agagaagtcc ttcacggaca cctctccccg gaatcctgcg cgcaccggct tgcccttcga
     2341 ggcaacgaac gaaacgtttc cttccgccga gatcgccccg cccgtcagca ggatcttcag
     2401 cttctccgtg taatacggct gcaagggtcc gatcggaagg gctttcgcct gcagcttggc
     2461 gttcatcgac ggcggatcga cggagaaggt gccgcccagg gagatactcc ccttccggtt
     2521 atagagggtg gcgaaggaga atttcccgcg ctgttttccg ccggtagcga cattctctgc
     2581 cttcagcctg agccggtcga gggtgatctc caccggcgga tccgtggtgc gatcctcgaa
     2641 cctcacggag taccggtcga cgaccgtttc cttaatggtg atcccccatg gcttttccgc
     2701 cggcctctcc ccatcggatt tcgtctgcgc gcctatcgcg ccggtcggct tcgcggataa
     2761 cccgtcgtcc ggcacaaggt gcgcgacgtt cgattccccg ccggaggaac gacgcacggc
     2821 aatccatcct ttcgccgtgg caatcccccc gatcgtgatc tctttcttga cgaggtcgat
     2881 ttccgcctct ttcacatcga acctggggat tacgaggaac tcctccttct cctcccgctg
     2941 acgcagacga aggtccgata cggacgctcc aaggccggag agtcggaatt ccggcccgct
     3001 atccccctgg gtaaaactgt agccggatcg cacgtcgagg gtcccgccgt tgatatcgaa
     3061 ccgcacctcg ttgctgaagt acggcgcata ctttttcagg accactttcg cgagggaaat
     3121 cgttccttcc gatccaaggg gagagaggga caggtttccc ttcagttcgg cggtctcccc
     3181 cgcttcggtc gaaaaggata ccagggtggc tgccttcttc cccacttcgg tgccaaggcc
     3241 gttcacatcg acccggatat ccgccaactt cgtcacgaag ggtatcgccc gggaggcgtc
     3301 ggcaaaccgc actttccccc cggcaagacg gatggaatcg acggcatatt tctggacctt
     3361 tgcgcccggc tcctctttcg gagcgttctt tccccccttt tccactactg cactttcctt
     3421 ttgtttttaa ggaactaacg ataataagtt caactttccg ctacgatcca gggagatgtc
     3481 gatttcgggg tcccgaacct gcagggacgc caggtgaaaa tcacgtgcgg caagatccga
     3541 gggagaaacc accgccttga ccatgggaag gcggatcatg gggctcttgt ccttcccgga
     3601 gacgcggact tccttcaggg tcacatcccc ttccgcagaa agggtggggg gcttgttttt
     3661 atgctgcgtg aaggaaatga ccgctttcac gtccaggaaa gcggatagga tctcgtattc
     3721 ccgttgcaag ggaacatatt caaggtagtg cggaatgtcg aggtccgaga tgttcacgtc
     3781 gaaggtggtt tccagcgact cgctgaacgg cttggtcttt cctttgagtg cgatcggctc
     3841 accgttcaca accgcagcga aggaaggttg cacgtaccgg tcgacgtaat aacggaggtt
     3901 cgaaataaac gggacgccaa tccggatccc ccggatttcg tgacgggtct tcttcggcct
     3961 gtcgtcgaag tccatgctcc cggcaataat ctggatatta ttaaaggaat actttagcga
     4021 tttgctttct tttgccggtt tttttatgaa ttgatcaatt agatccgtga agttgtatga
     4081 tccgtccggg cgccgcacga tgttcacgta cggccgggac aatcggatct cgctgagcac
     4141 ggggcctccc cgcatcaccg acgccagttg aaggttcgcg aagacctcct ccacggagac
     4201 ccaggttccc ggggagtcgc gctcggagat cgcgagcccc cggacggaga cggacagttc
     4261 gaagggattt acccggatgt cccggagcat cgctttccgg tggagcgttt cggtgaggga
     4321 tttcgaaagg acggatttga ggatgggcgg gagggcgagg aacccgaaga gggcataaac
     4381 aagaagggct gccacccccg cgatcaagcc cttccggacc atgggcttcg accggatatg
     4441 cttgacccat ttcatcgcgg gtctcccctc gttcgccgcc tcctaccgcg ttcctacatc
     4501 catctttggg aatgaggtat gcctacaccg cctccatcgc cggggaatag ttcccgtgcc
     4561 gcgcggcccc gttcagcttt gctcgcaggt ggaacgcctc cttcgccgca gggacgttcg
     4621 ccgcctttcc tttccaggcc tttaacaccg gatcctgcag cgccctcccg taggagaaac
     4681 tcagttccca cggatgcttc cccatcgcgt tcatggcgct cagatgctcc gtggcctgac
     4741 gaggcgcctg gcctccggag aggaagacga tgccggggac ggccggaggt acgacgcggg
     4801 agagggttcg gaccgtcgct tccgcgacct gcgccgcttg ggcctgggcc ggacacccct
     4861 tccccggaac gaccatgttg ggcttcagca acgttccctc gagggccacg cggtgagccg
     4921 tcaactccgc gtagacgagc gagagcacct cggtggtcac ctcctcgcac cgctcgatcg
     4981 tatggccccc gtccatcagc acttccggtt caacgatggg tacgaggccc gcttcctggc
     5041 acagcgcggc gtatcgggca agcgcgtggg cgttggcctg gatgcagtat cgcgtcggga
     5101 tccctgcccc gatgtcgatc accgcgcgcc acttcgcgaa acgggcgccc agcgcccgat
     5161 attccggcag ccgtccccgc agcccgtcca gcccctcggt gatcttctcc ccgggaaacc
     5221 cgatcagcgc tttcgcccct tcgtcgacct tgatccccgg cacgatcccc tgccgttcaa
     5281 gcaccttcgt gaacggcgtc ccgtcggcgg agcattgccg gatcgtctcg tcgtaaagga
     5341 tcacgccgct gatgaattcc gaaacgcgcg gagtcgtgaa gaggaggtcc cggtacgctc
     5401 tccgattctc ctccgtggac gggaaatcga tcgccttgaa ccgtttctcg atggtcgggg
     5461 cgctttcgtc cgcggccagg atccccttgc ctgggacgac gagggagcgc gcgaccgatt
     5521 ccaactcgcc gaggttcatc ttcatacctc ctgggtgatt tccactccat ctactggatt
     5581 cccccgggga catccgggtt tcggattgtt atactccatt aaaatatcgg aatccaccgc
     5641 acggtggggg gacgacgtgg cggaacgccg tgacgatccg gttctggtct actccacgga
     5701 gcgggggctc gtctgcccga aatgtcgcct gcccatggcc aaatgccgct gcgcaaaaga
     5761 ggaacctgcg cccgcgggga acgggatcgt ccgcgtccgc cgtgagacga agaaccgggg
     5821 aggaaagacg gtgaccaccg tatcgggcgt cccgctcggg ggggaagcgc tccggaacct
     5881 tgcgtcggac ctgaagcgcc gctgcggctc cgggggaacg gcgaaggacg gtgtgatcga
     5941 gatccagggg gaccaccggg agacgatcgt ggaggaactc ttgcaccgcg gcttcaccgc
     6001 gaagctcgca ggcggctagc cccccggccc cggaatacgg ccactattga tggcctcagg
     6061 cttgtaattt cttatcaaaa ttgatattca ttctatagcc gtttttcttc caccgaaggg
     6121 tcagacatga aagtcctgct gctctatccc gaatttcccg atacgttttg gagttttcgc
     6181 cacgccctcc cgttcatcgg gaaacggtcc gcctatcctc ccctgggttt gctgaccgta
     6241 tcggcgatgc ttccgccgca ctgggaacgg aaactggtgg acctcaacgt ggaggagctc
     6301 cgcgacaagg acatcgcctg ggccgacgtg gcattcctta gcgcaatgct ggtccaggga
     6361 tcttcccttg cgcagttgat cgcccgttgc cgggaagcgg ggctgcggac ggtggtgggc
     6421 ggaccggtca cgagcgccga caacccttcg tacgagggtg cggaccacat cgtccggggc
     6481 gaagcggaag ggatcatcga ggagattgtt tccgaccttg aagcgggcaa tgcgcgccgc
     6541 cattacgaag ccgtcggccg ggcggacatg accgtagtcc ctccccccga tctgcgcctc
     6601 gcgagattgc gacgctacag cgcgatgtcc atccagtact cccgggggtg cccgttctcc
     6661 tgcgagttct gcgacgtcat cgaactcttc gggcgaatcc cccgcaccaa gacggtggag
     6721 caggtcctgg aggagtttga actcctctat cgcatggggt ggcgggggtc ggtgttcgtc
     6781 gtggacgaca acttcgtcgg gaacaggcag gccatcaagg acctcctgcc acggatcgcc
     6841 gattggatgc gcagccgggg caaccccttc tccctgttca cccaggcgtc gatcaacctg
     6901 gccgaagacg acgaactgct ctccctgatg cggacggccc gcttcaacaa ggtgttcata
     6961 gggatcgaaa ccccctctgc ggaatgcaac cgggcggcgg gcaagatgca gaacgtgaag
     7021 gccgatctat tggcgtgcgt gaggcacatc caggagcacg ggatggaagt gatgggaggt
     7081 ttcatcgtcg gcttcgatca ggaccccccg gagatcttcg agaggcagat cgccttcatc
     7141 agggaggcgg ccatccccat ctccatggtc ggcgtcctga tcgcgcttcc caacacacgg
     7201 ctgtggcggc ggctctccga agagggccgg atcttgcggc agacaatggg gaacaacacg
     7261 gaagccttgc tgaatttcat ccccaggatg aatcccgaag cactcctcgc cggataccgg
     7321 aaggtgctgg cttccatcta cagcccgtcg gaatatttcg agcgggtcca ggtgatgatc
     7381 tcccggctgg ggg
//
LOCUS       DASVJL010000277         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_981933, whole genome shotgun sequence.
ACCESSION   DASVJL010000277 DASVJL010000000
VERSION     DASVJL010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981933"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VF357_05845"
     CDS             <1..985
                     /locus_tag="VF357_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX7523702.1"
                     /translation="RSGAVFPVLAAALLGALALSAATGPVSISPRAAFDAIFRGTDDT
                     PARVLLSLRLPRALLGALVGGALASSGVAFQALLRNPLADPYILGVSGGAAVGALTVA
                     LFLSVDASPLLPLCAFAGAALSALAVFLLARRRSGVSPERLILMGVVVGAFLNAVIML
                     MVTLAPPGKIPGALYWLMGDLGLGTPRRVAVLFPYVALGTVLLYLLSRGLDLLLLGDH
                     AAFQAGLSVERVKTATYLTASLLAGSVVAVSGLIGFVGLIVPHGARVLVGSGHRRLLP
                     AAFLLGGAFLVLSDTLARAASPAGELPVGAVTALAGAPFFLYLLRRNGGRS"
     gene            982..1788
                     /locus_tag="VF357_05850"
     CDS             982..1788
                     /locus_tag="VF357_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523703.1"
                     /translation="MNPVLSARGVGFGYGDRDILRGVNVDLSPGEVTILLGPNGAGKS
                     TLIRILSGILPPREGEVLLCGRSPGEFRRREMARLLSVVGQDPPVDFPMTVAAYVGLG
                     RFPHQGFFGGTSPEDREQVERAMERTELDGLRDRTLAEISAGERQRAAVARAIAQGAR
                     VMLLDEPTAFLDIRHRVAFYEIVTQLKETSGIAALVASHDLSLCAEYGERIVLLSGGT
                     VAAQGRPEEVLTPGNVQAAYGVAVACDRNPATGSIRVTPLRGMPLRDRDR"
     regulatory      2019..2204
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2293..>2696
                     /locus_tag="VF357_05855"
     CDS             2293..>2696
                     /locus_tag="VF357_05855"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7523704.1"
                     /translation="MGRMVVLLVASWLTGSVGAMAQEPVRIEPVVVTATRIEEKVSEQ
                     ASSVSVVTREDMDRKSPSLAGDVLQGIPGVDVQRTGSLGGLENIKIRGGKSTHTLVMI
                     DGFPVNSPSSGEFDISALPVNGFERVEIVRGA"
BASE COUNT          374 a          895 c          981 g          446 t
ORIGIN      
        1 aaggagcggc gcggtttttc ccgtgctcgc cgccgcgctc cttggcgcgc tggccctctc
       61 cgcggccacc gggccggtgt cgatctcccc gcgcgcggcc ttcgacgcga tcttccgggg
      121 aacggacgac actcccgccc gagtcctgtt gtcgctgcgg ctcccgcgag ccctcctcgg
      181 cgccctcgtg ggcggggccc tcgcctcttc cggcgtcgcc ttccaggcgc tgctgcgcaa
      241 tccgctcgcg gatccgtaca tcctcggtgt atcggggggc gccgcggtgg gcgcgctcac
      301 ggtggccctt ttcctgtccg tggacgcgtc cccactcctt ccgctgtgcg ccttcgccgg
      361 agcggcgctg tccgccttgg ccgtcttcct cctcgcccgc cgtcgtagcg gcgtttcccc
      421 ggaacggctg atcctgatgg gtgtcgtcgt tggagcgttc ctcaacgcgg tcatcatgct
      481 gatggtgacc ctcgccccgc ccgggaagat ccccggagcc ctgtattggc tgatggggga
      541 cctgggcctg ggaactcccc gccgcgtggc ggtcctcttc ccgtatgtcg cgctcggcac
      601 ggtcctcctg tacctgctct cccgcggcct ggacctgctc ctcctgggcg atcacgccgc
      661 gtttcaggcg gggctctccg tggagcgggt gaagacggcg acgtacctca cggcgtcgct
      721 gctggccggg tcggtcgtgg cggtctcggg cctcatcgga ttcgtcgggc tcatcgttcc
      781 ccacggcgcg cgcgtgctgg tcggatccgg gcaccgccgc cttctgccgg ccgcgttcct
      841 gctgggcggc gcgttcctcg tcctctccga cacgctcgcc cgcgccgcgt cccccgcggg
      901 agaattgccg gttggggcgg tcaccgccct cgccggcgcc cccttcttcc tctatctgct
      961 gcgccgcaac gggggacggt cgtgaacccg gtgctgtccg cccgtggagt gggattcggc
     1021 tacggtgacc gcgacatcct gcgcggtgtg aacgtcgacc tctcgccggg agaggtgacg
     1081 atcctcctcg gtcccaacgg ggccgggaag agcaccttga tccggatcct gtcggggatt
     1141 ctcccgcccc gcgaggggga ggtgctgctg tgcggtcgct ccccggggga gttccgccgc
     1201 cgggagatgg cgcgcctcct ctccgtcgtg gggcaggacc cgccggtcga cttcccgatg
     1261 acggtcgccg cctatgtcgg cctcgggcgg ttcccccacc agggcttctt cgggggaacg
     1321 tcgccggagg atcgggagca ggtggagcgg gcgatggaga ggacggaact ggacgggctg
     1381 cgggaccgga ccctggcgga gatctccgcg ggggagcgcc aacgcgccgc cgtggcccgg
     1441 gcgatcgcgc agggggcgcg ggtgatgctg ctggacgaac cgacggcgtt cctcgacatc
     1501 cgccatcggg tcgcgttcta cgagatcgtc acgcaattga aggagacgtc cgggatcgcc
     1561 gcgctggtcg cctcccacga cctgtcgctg tgcgcggagt acggggagcg gatcgtcctg
     1621 ctgtccgggg ggacggtcgc ggcgcagggg cggccggagg aggtgctcac tcccggcaac
     1681 gtgcaggccg cgtacggggt ggcggtcgcc tgcgaccgga accccgccac cggctcgatc
     1741 cgcgtcaccc cactgcgggg aatgcctctc cgggaccgtg acagatgaat atgctacgca
     1801 gggttactcg cgggcggctt cctctccgct acggctcgtc ggggcactga cgtagggggc
     1861 cttcccccga gcgggcgtct ggagcgagag aagccaggga tggcgggaac gagcggaggc
     1921 gcagtgaagg cggccaggga aggccgccga acgagagcga gcggggaatg gcctccgagg
     1981 aagaggggac cccgccgcaa gactggttga agaagcgcag caaacggaac atcaaggttc
     2041 cacccagggc ggaaccggga aagacggtgc gaatccgtcg ctgccccgca tctgtcaccg
     2101 gaacgaaacc cgcacggagc cactgtttcg catcgcggaa tgggaaggcg cgggaagtag
     2161 gtcgccggaa gccagaagaa ccgggctgcc ctgcattaac ccacaccctt tcgagggaaa
     2221 ggaggagcaa tgcagggatt caacagggta cgtctcaggg aacggggaag cgcagcgggg
     2281 ggattccatg ccatgggacg catggttgtg ctgctcgtcg cgtcatggct aacgggttcc
     2341 gtgggcgcga tggcgcagga gccggtgcgg atcgagcccg tcgtcgtcac ggcgacgcgg
     2401 atcgaggaga aggtctcgga gcaggcgtcg tcggtcagcg tggtgacgag ggaagacatg
     2461 gatcgaaaga gcccttccct ggcgggcgac gtcctgcagg gaatccccgg ggtcgatgtc
     2521 cagcggaccg gaagtctcgg gggtctggag aacatcaaga tccgcggggg gaaaagcacc
     2581 cacaccttgg tgatgatcga tgggtttccc gtgaacagcc cctcatcggg agagttcgat
     2641 atcagcgccc tcccggtgaa cgggttcgag cgtgtggaga tcgtccgcgg cgcgca
//
LOCUS       DASVJL010000278         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_794907, whole genome shotgun sequence.
ACCESSION   DASVJL010000278 DASVJL010000000
VERSION     DASVJL010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794907"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VF357_05860"
     CDS             complement(<1..468)
                     /locus_tag="VF357_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7523705.1"
                     /translation="MRFKLLGRTGLRVSELCLGTMTFGEEWGWGASKEESRNIFDAFA
                     DAGGNFVDTANLYTGGTSEKFVGEFLKGRRERFVLATKYTLNMAPDDPNGGGNHRKNL
                     VQSLDASLKRLGTDYIDLYWVHAWDGMTPLEETMRALDDVVRAGKVLYVGISDS"
     gene            732..1490
                     /locus_tag="VF357_05865"
     CDS             732..1490
                     /locus_tag="VF357_05865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEX7523706.1"
                     /translation="MSERFRLKAREWLDVPERKRVYNERHFAEAASRYDLATRMMSLG
                     RDAAWKRMLVGSLPSLPCPVCVDLACGTGDVAFLLAGRYPGGVVTGVDLSAPMLAIAR
                     ERNRFANVRFEQGDMCDLPLPDRSADVVTGSYALRNAPDLQKAIAEVHRVLSPGGVAA
                     FLDFSNPESSLLRHLQYLLLRSWCGFWGLLLHGTPEIHGYVAESLRVFPARNRLREIF
                     REQGLGMCADRHFFLGITGLLILRKGEPPASVGV"
     gene            1557..1745
                     /locus_tag="VF357_05870"
     CDS             1557..1745
                     /locus_tag="VF357_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523707.1"
                     /translation="MEITLTDAEKLVLRETVEKATHEMLMEIANTDNRKMREGLRERE
                     EILRTIFAKLPAEARTAA"
     gene            1932..>2785
                     /locus_tag="VF357_05875"
     CDS             1932..>2785
                     /locus_tag="VF357_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7523708.1"
                     /translation="MALPWNIPSQRQSPAPPGADGNVPVQIDRNDPNQQRLLISLLDN
                     IPGMVYRGLPDWSLSFVGSEVERLTGYTPEEILAKPKVWRGLIHLDDFRSVREAILSC
                     VRRREPILRMEYRIVRKDGESRWISDRRQMIYDEGGRLLCIDGLCLDISEQKRDEEEK
                     RRLTAAVEHAADAIMVTDAEWIVQYVNPAFEKVTGYAKEEVLGKNPYILAADGENIRV
                     YIGIEEKIRTGSPWKGRLKNKRKDGMLLELDTVVSPIRDPGGKIVNHVVASRDITREV
                     QLEKQARAA"
BASE COUNT          529 a          858 c          896 g          502 t
ORIGIN      
        1 gctgtcggag atcccgacgt agaggacctt cccggcccgc accacgtcgt ccagggcccg
       61 catcgtctcc tcgagggggg tcatcccgtc ccaggcgtgg acccagtaga ggtcgatgta
      121 gtcggtgccg aggcgcttca ggctggcgtc gagcgactgc accaggttct tccggtggtt
      181 ccctccgccg ttcgggtcgt cgggggccat gttcagggta tatttcgtgg cgaggacgaa
      241 ccgctcccgc cggcctttca ggaactcgcc cacgaacttt tcgctcgtgc cgccggtgta
      301 caggttcgcg gtgtcgacga agtttccacc ggcgtccgcg aacgcatcga aaatattgcg
      361 gctctcttct ttcgacgctc cccagcccca ctcctcgccg aacgtcatcg tccccaggca
      421 cagttccgag acccgaaggc ccgtcctccc cagcagcttg aatcgcatcg cgcgcctcct
      481 ttcgaattcc tcggaaataa ggttatggaa tatagatgaa cgctataggc aatccgtcgc
      541 ttcccgcagc gcctcttcct cgatccgggc tcgccgccgc aggatggcga gtcccgatcc
      601 ccttgagacg catccaacca aatatatcgg ttcataaaca aggaggcgat ggtgaaaatt
      661 gcaggagtgg gcccgcgacg accggatgcc gcaggggtcg cgtcctccgc cgcgatcccc
      721 cggttgacgg gatgagcgaa cgattccgcc tcaaggcgcg ggagtggctc gacgtcccgg
      781 agcggaaacg ggtctacaac gaacgccact tcgccgaggc ggcgtcgagg tacgacctgg
      841 cgacccgcat gatgtccctg gggcgggacg cggcgtggaa gcggatgctt gtcggctccc
      901 tgccgagcct cccgtgccct gtttgcgtgg atcttgcgtg cggcaccggc gacgtcgcct
      961 tcctcctcgc cggacggtac cccggcgggg tcgtgacagg agtggatctc tccgcgccga
     1021 tgctggccat cgcgcgggag cgcaaccggt tcgcgaacgt acggttcgag caaggcgaca
     1081 tgtgcgatct cccgctcccc gatcgctccg ccgacgtcgt gacagggagc tacgcccttc
     1141 gcaacgcgcc cgaccttcag aaagccatcg ccgaggtcca ccgggtgctt tccccgggtg
     1201 gcgtggcggc gttcctggat ttttccaatc cggaaagctc cctcctccgg catctgcagt
     1261 accttctgct gcggagctgg tgcggtttct ggggcctcct cctccacggg acgccggaga
     1321 tccacgggta tgtcgcggag agccttcgcg tcttcccggc ccggaaccgg ttgcgggaaa
     1381 tcttccggga acaagggctc gggatgtgtg ccgaccggca cttttttctc ggaatcacgg
     1441 ggttgctgat actgcggaag ggagagcctc ccgcgtccgt tggagtataa taagtacatg
     1501 gaagtggata tcggccggca acggaacgcc tctacgggtt cgaaggaggt gtgagtatgg
     1561 aaatcaccct gaccgacgcc gagaagcttg tcttgcggga gaccgtggag aaggcgaccc
     1621 atgagatgct gatggagatc gccaacacgg acaaccggaa gatgcgtgaa ggcctgagag
     1681 aacgcgagga gatcctgagg acgatcttcg cgaagcttcc tgccgaggca cgcaccgcgg
     1741 catgagcgca cccccgtcgt aacggggaga gaatgacggg gaaaggacgg gaccggttcg
     1801 ggcgtcctgt gggacctccg gggcgggagt tccaggtcga ttcattttca gacccttcgc
     1861 cggaattcat gtaaagattt gcccccttcc tgccgaaaca tcatttgata gtttcatctg
     1921 gaggggccgc attggcgcta ccgtggaata ttccgtcgca gaggcaaagt cccgcgccgc
     1981 cgggtgcgga cgggaacgtc ccggtacaga tcgatcgcaa cgaccccaat cagcaaagac
     2041 tccttatctc ccttctcgac aacatccctg ggatggtgta ccgggggttg cccgactggt
     2101 cgctttcgtt cgtcggatcc gaggtggaga ggctcacggg gtacaccccc gaggagatcc
     2161 tcgccaagcc gaaggtatgg agagggctga tccaccttga cgatttccgg tcggtgcggg
     2221 aggcgatcct gtcctgtgtc cgccgacggg agccgatcct ccggatggag taccggatcg
     2281 tccgcaagga cggggagagc cggtggatca gcgaccggcg ccagatgatc tacgacgaag
     2341 gcggcaggct cctctgcatc gacggactct gcctcgacat ctcggagcaa aagcgcgacg
     2401 aagaagagaa acgccggctt accgccgcgg tggagcacgc ggcggacgcc atcatggtca
     2461 ccgatgcgga gtggattgtc caatatgtca accccgcctt cgagaaggtc acggggtacg
     2521 cgaaggagga ggtcctcggg aaaaatccgt atatccttgc cgcggacggg gaaaatatac
     2581 gggtctacat cgggatcgag gaaaagattc ggaccggatc tccgtggaaa gggcgcctga
     2641 agaacaagcg gaaggacgga atgctcctcg agctggacac cgtcgtctcc ccgatccggg
     2701 atcccggcgg aaagatcgtc aaccacgtgg tggcctcgcg cgacatcacc cgggaagtcc
     2761 agctcgaaaa acaggcccgg gcggc
//
LOCUS       DASVJL010000279         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_982624, whole genome shotgun sequence.
ACCESSION   DASVJL010000279 DASVJL010000000
VERSION     DASVJL010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982624"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..768)
                     /locus_tag="VF357_05880"
     CDS             complement(25..768)
                     /locus_tag="VF357_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523709.1"
                     /translation="MPKVLSVSRLRRVFHDTIAVDGISFEVGRNEIVGLLGPNGAGKT
                     TTINMILGVLDPSSGTIRVGGADISANRALALEQTNFAAVYAPLPGNLTVSQNLRVFG
                     RLYGVKDLLKRIENLLGQFDLKEREHVKCGVLSSGEQTRLSLVKALLNRPRLLLLDEP
                     TASLDPATARDIRARICEYAAQDQVGVLWTSHNMYEVADVCDRVLFLSHGRILLEGDP
                     RTLPQEHGKETLEELFITAAREPLAFEQG"
     gene            949..2085
                     /locus_tag="VF357_05885"
     CDS             949..2085
                     /locus_tag="VF357_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX7523710.1"
                     /translation="MESWFDEVVIGRIPMRNRIVRSATWEGMCEKDGAPTERLVALYE
                     ELTEGGVGLVITGYAYVRPEGKQAPGKLGIYDDSLVTGLKSVPRHVHDRGGRIVAQLV
                     HAGGQADRKVTGLPPVAPSAVSVPYYPEIPKELPPEEIRDIVAAFGRAAGRAKEAGFD
                     GVQLHGAHGYLINQFLSPLTNRRTDAYGGPLQNRSRFFSEVLHAVRAAVGEEYPVWIK
                     LNGEDFLPGGFSLEEACQVARTLEEEGIDAIEVSGGTTASREKIPARTRIDAPEKEAY
                     HRALAREIKDRVRIPVGLVGGLRSPAVLEEILQASDADFFSMARPLIREPALVNRWSS
                     GDRSTARCISCNGCFLPGFKEGGIYCVVERKSTSTGRSVPLTGA"
     gene            complement(2072..2980)
                     /locus_tag="VF357_05890"
     CDS             complement(2072..2980)
                     /locus_tag="VF357_05890"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX7523711.1"
                     /translation="MTVLRAIRKVWRSRPTIEGAGVHLKRAFGNQEAPRLDPFLLLDD
                     FRSDDPSKYLPGFPWHPHRGIETITYVLQGNVEHGDSMGNRGDITPGDVQWMTAGSGI
                     VHQEMPKGDAQGRMGGFQLWANLPASEKMMDPRYRDVKRSEIPEVSMEGGAKVKIVCG
                     RVNGVQGPVRDIVTDPEYLDVAVPKGARFTHPVERGHTVFAYVIQGSAYFDKGRDPYA
                     REEAGRNYFDMDRECQCGPESLVHYGEGDAVSITADKEPVRFLLVSGKPIGEPVAWYG
                     PIVMNTSEEIRIAFEEYRNGTFIKHQ"
BASE COUNT          616 a          994 c         1072 g          640 t
ORIGIN      
        1 agattcggtg atatcgcatt gcgatcaccc ctgctcgaag gcgaggggtt cccgcgccgc
       61 ggtgatgaag agttcctcga gcgtctcctt gccgtgctcc tgcggcaagg tcctcgggtc
      121 tccttcgagc aggattcgcc cgtgcgacag gaagagcacc cggtcgcaga cgtccgcgac
      181 ctcgtacatg ttgtgcgagg tccaaagcac ccccacctgg tcctgcgcgg cgtattcgca
      241 aatcctcgct cggatgtcgc gcgccgtcgc gggatcgagc gaggccgtcg gttcgtcgag
      301 aagcaggagg cgcggccgat tcaacagcgc cttgaccagg gacaggcggg tttgctcccc
      361 cgaagagagc acgccgcact tgacgtgttc ccgctccttg agatcgaact ggcccagaag
      421 gttctctatg cgcttcagaa ggtccttcac cccgtacaac cgtccgaaga cgcggaggtt
      481 ctgtgacacg gtcaggttac cgggcagggg agcgtagacc gccgcgaaat tcgtctgctc
      541 aagcgccagg gcccgattcg ccgagatgtc cgcgcctccg acccggatcg ttccggaact
      601 cggatcaagg acaccgagga tcatgttgat ggtggtggtt ttccccgctc cattgggccc
      661 caggagaccg acgatctcgt tccgtcccac ctcgaacgaa atgccgtcga ccgcgatcgt
      721 gtcatggaag acccgccgca accgtgagac cgagagcacc ttcggcatca ctgtcccacc
      781 cccatccgga atcgtcccca agtttacggc gaatcaagtc atgggtccaa tgtcatggag
      841 gtcgtccatc cctttcttcg caggcgcggt aaacgggacg aaattgatcg tcatgggata
      901 aaatgaaaaa attatcggaa atcgcgtcgg gaagaaaggg gctgaggcat ggaatcgtgg
      961 tttgacgagg tggtgatcgg tcgcattccc atgagaaacc gcattgtgag gtccgcaacc
     1021 tgggagggta tgtgcgaaaa ggacggcgcc ccgacggagc gcctggtcgc actgtatgaa
     1081 gagctgacgg aaggaggggt agggctcgtc atcacggggt acgcgtacgt ccgacccgaa
     1141 gggaaacagg ctccggggaa gttggggatc tacgacgatt cgctcgtgac cggattaaag
     1201 agcgtgcccc gacacgttca cgaccgcgga ggaaggatcg tcgcgcagct cgtacacgcg
     1261 ggtgggcagg cagatcggaa ggtaaccggc cttcctcccg tcgcgccgtc cgcggtgtcc
     1321 gtaccgtact acccggagat ccccaaagaa ctgccgccgg aagagatccg ggatatcgta
     1381 gccgccttcg gccgggccgc cgggcgggcg aaggaagctg gttttgacgg ggtccaactc
     1441 catggcgccc acgggtatct catcaaccag ttcctttcgc cactgacgaa ccgccggacc
     1501 gatgcgtatg gtggtccgtt gcagaatcgc agccggttct tctcggaggt gctccatgcc
     1561 gtccgcgcgg cggtggggga agagtacccg gtctggatca agttgaacgg ggaggatttc
     1621 ctcccgggag gtttttcgct ggaggaagcg tgccaggtcg cccggacgct cgaagaggaa
     1681 ggtatcgacg cgatcgaggt gtccggaggg acgacggcat ccagggagaa gattcccgcc
     1741 cgcacccgga tcgatgctcc ggagaaggag gcataccaca gggcgctggc gcgggagatc
     1801 aaggaccggg ttcgaattcc cgtcgggctc gtggggggac ttcgaagccc tgccgtactg
     1861 gaagagatcc ttcaggcgag cgatgccgac ttcttctcga tggcccgtcc cttgatccgg
     1921 gaacccgccc tggtgaatcg gtggtcctcg ggggaccgat ccacggcccg ttgcatctcc
     1981 tgcaacggtt gcttcctccc ggggttcaag gagggaggga tctattgcgt ggtcgaaagg
     2041 aaatcaactt cgaccggacg ctccgttcct ctcactggtg cttgatgaag gtcccgttcc
     2101 gatactcttc gaacgcgatc cggatttcct cggaggtgtt catcacgatg gggccatacc
     2161 atgccaccgg ctcgccgatc ggtttgccgg acacgaggag aaagcgaacc ggctccttgt
     2221 cggcggtgat ggaaacggcg tcgccttcgc cgtagtgaac aaggctctcc ggtccgcact
     2281 ggcactcccg atccatatcg aagtagttcc gtcccgcttc ctcgcgggcg tacggatccc
     2341 ggcccttgtc gaagtaggcg ctcccctgga tgacgtacgc gaagaccgta tgtcctcttt
     2401 cgaccgggtg agtgaaacgc gcccccttcg ggacggcgac gtcgaggtat tcggggtcgg
     2461 tgacgatgtc ccggaccggt ccctgcacgc cgtttacccg accgcagacg atcttcacct
     2521 tcgcgccgcc ttccatggag acttcgggaa tctcgcttcg cttcacgtcc cggtaccgag
     2581 ggtccatcat cttctcggag gcgggcaggt tggcccagag ctggaatccc cccattcgtc
     2641 cctgtgcgtc gcccttgggc atctcctggt gaacgatgcc gctgcccgcg gtcatccact
     2701 ggacgtcgcc cggggtgatg tcgccccggt ttcccatgct gtccccgtgc tccacgttcc
     2761 cctggagaac gtaggtgatc gtctcgattc cccgatgcgg gtgccagggg aatccgggaa
     2821 ggtatttcga ggggtcgtcc gagcggaaat cgtcgaggag caggaagggg tcgaggcggg
     2881 gcgcctcctg gttgccgaag gcccgcttga ggtggacgcc cgccccttcg atcgtcggcc
     2941 tggaccgcca cactttccgg atggctctga ggaccgtcat catcacacct ccatgggcac
     3001 tgccccccgt tgtcgccggg tgcggtggat atcgcctcga atgcatggtt gcacacccat
     3061 cagggaatat aggccggaag ggacgggagg gtcaatcgtt ccggcgtggg gcaggacgaa
     3121 cgatgcgttg tcattcgggc gggattgtgg gaaattgact ccgaggacca ggaatcaacg
     3181 gcacgtcact ggaacctaaa ggagggattc ggcgatgcga aaaagatcac tgttcggtgt
     3241 ttgtttcctc gtgattgtca tggcgttctc ccttactggc tgggttcagg cagagacgat
     3301 caagatcggc ataaacgccg ag
//
LOCUS       DASVJL010000280         9410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1264283, whole genome shotgun sequence.
ACCESSION   DASVJL010000280 DASVJL010000000
VERSION     DASVJL010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264283"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VF357_05895"
     CDS             complement(<1..256)
                     /locus_tag="VF357_05895"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7523712.1"
                     /translation="MKTFDEDLVKDRVNRFPFMKMMGMKLISCSGGTSVMQCRVRQAL
                     KNSGGTLHGGVLGTLVDMSVATALRSALPLTSRMTTVEYKV"
     gene            complement(273..2294)
                     /gene="fusA"
                     /locus_tag="VF357_05900"
     CDS             complement(273..2294)
                     /gene="fusA"
                     /locus_tag="VF357_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX7523713.1"
                     /translation="MASAVNLRNIGIIAHIDAGKTTFTERLLYYAGITHRMGEVHDGD
                     SQMDYLPQERERGITITAAATHFSWMGAEVHLIDTPGHVDFTIEVERSLRVLDGAVAV
                     FCGVGGVEPQSEVVWRQADRHGIPRLAFVNKLDRPGADFDRVVDDMARKLDARGVPLT
                     VPLCENGTFVAVADLVTMEKVSFSAEDLGAGVSRAPLSEAEGLSCARYREALLEAAAD
                     VDDAAAEKYLAGETVPVPLLRAALRKGTLAGKIFPVFAGSALRNRGVQPAMDGIVLYL
                     PSPEEARPARGDDPRTGVPATREPVPSAPFSALVFKVLQEEGRRTVYLRVYSGKAKEG
                     DTLLNAATEQEEKVARLFRMHAGKKERIAVAAAGDIVAARGIRFARTGDTLCDPGAPI
                     VYESIEIRKPVMSVVVEPRTVREMDRLRELLGAMTDEDPTLSFREDADTGQILLSGMG
                     ELHLEIAIDRLAREHGMEVRKGNPQVLYRETVASAGRAESVFEREIAERQVKVVTVVA
                     VSPAQRGSGVRISDGFRLLGLPPDIADGAEMGLREGAFFGSLGYPVDDATIELSRLEF
                     LSGVPSPMAAKVAAAKAFHEAYEKGKPYLLEPVMAVEIVVPDEFLGGVIGDVNARRGK
                     VSSVDRRPGGSFLSAAIPLKEMFGYVTALRSLSQGRGTYTMKFSHYDKG"
     gene            complement(2296..4404)
                     /locus_tag="VF357_05905"
     CDS             complement(2296..4404)
                     /locus_tag="VF357_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX7523714.1"
                     /translation="MNTLLTEADLPEAIGLLHQVAPLQPDAIIAADLGLVRILHEFFP
                     GIPVHMSTQAGCASAEAAEEFARMGASRVILERHLRKEEIARIAERSPAGVEIFVHGS
                     MCYSYSGKCFFSSYLGGKSGNRGVCVQPCRRLYGHGGEPEAIFSTRDLSLLPHLPDLV
                     PLGIASLKIEGRMRGAEYVAGVVSAYRAALDGIRAGNPVEGVAEGTRILSQVIGRETT
                     PGLAGGARPGEVATGGESGNVGELVGTVARVEDGWAFVPGAAGISPGDRLRAQFREDG
                     AGRGFSAIDPRCEEGGLRVKVPFPVSPGDLLFRVGGTGRAEFTRLARKEMETTPSDGA
                     RFVVSVSPGTVTVKASYGNEEKVFVYRISGPPGGPPGTVPLDGERQLADAYRGDLPLA
                     GVRVEIHGGPGVWGDVRTLFLQAARQFDREFYLAGKRLRVEILPTLRVSGSRPEEGPG
                     TVIFAGCRPEQLPHLPKTPEVVPVVEFTRSLARDPSPAARYARSGGFLRLPSPMLESD
                     AAFLRRTVTDAIRKGFTRWVVSDAGHFRLFAPAPLRRQVTLISDHYLYAFNMAALAAL
                     SRMGATRMILPVEATVPALRDVGKFLYGLGIAVAYGPVPLMISRLLPAAGVRAGEVES
                     PRAERFRVTADEHGSIILPAEPFSASGSLHVLREAGIRDFFADLRGLGPAEITEVLSA
                     LSADREIPGTSTFNLLRRNF"
     gene            4990..5538
                     /locus_tag="VF357_05910"
     CDS             4990..5538
                     /locus_tag="VF357_05910"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX7523715.1"
                     /translation="MAYIPLVLLVVIVAWFVAAYNKLVRYRNQIKNAWHQIDVQLKRR
                     YDLIPNLVEVVKDYMAYEQETLTKVIEARGAALTAKGPAAQAKAEGLLTETLKSLFAV
                     VEKYPELKANQNVASLQEELTGTENKISFARQFYNDSVMTYNNAIQSIPTNFIASFFS
                     FAQEAYFETEPESRAVPKASLR"
     gene            5554..6651
                     /locus_tag="VF357_05915"
     CDS             5554..6651
                     /locus_tag="VF357_05915"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7523716.1"
                     /translation="MISGIAPACPKCGGANRPGADLCYLCGESLEAGPGANAFVGDAS
                     PLAEARPPLRPIPMLLFTEAARHNVRMSALLFVLLFAVFFLLGIAIGGAYGDPRTGLG
                     LSFVLYAILGTTAYFSGSSIVLSIHGAREAGGEEHRQLRNVVEEMAIAAGVPLPKAYV
                     IPAAGMNAFAAGRRPEEACVAVTTGLLDRLNREELQGVIAHELAHIKSRDTLYNVCAA
                     VLVGAVALLSDMFLRGAFWGRRGRTEGERGGGGRGNAAFFVLALLLAVLAPLAAKILQ
                     MSISRQREFHADAAAAGFTRNPLGLASALAKITAGGPEVPGENRGTQHLFIVNPLRVF
                     GPDDSALMSTHPPTEERIQRLRAMGGVAAGN"
     gene            6671..6886
                     /locus_tag="VF357_05920"
     CDS             6671..6886
                     /locus_tag="VF357_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:6707..6709,aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HEX7523717.1"
                     /translation="MPRVVTIFGKDTUPYTTNARRDYEKAGAAVEYRNVLKDPASLSE
                     MLRLVGGRRDVPVVLEGGKATVGYGGS"
     gene            6970..7449
                     /locus_tag="VF357_05925"
     CDS             6970..7449
                     /locus_tag="VF357_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX7523718.1"
                     /translation="MQFSEDALKFLNLGINSEIAAYVFYKHAGAIVTEKGLQDTLHRL
                     ANDEKGHFLSLEDIYDRNVRSEMWAPYKDILSKAGLPDIDELVQDTHKELLSRIRGIA
                     TKREVLEMALVLEKEAFTLFSEGAAKMKDPEIKKVFDFLTGFERVHVMLIEKELATL"
     gene            complement(7497..8849)
                     /locus_tag="VF357_05930"
     CDS             complement(7497..8849)
                     /locus_tag="VF357_05930"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HEX7523719.1"
                     /translation="MKGWKMLPVLLVLLFTASTSFAAGFRLPEAGAKAMGMGFAFTGQ
                     ADDPSAIYFNPAGLTQLKGQNVMVGVTYIRENGATFTGTTPLTLNTGTGSFDSKSETQ
                     KSLDFFIPNLYYTKTTSDGYFAYGVGIFSPFGLGQEYNSKTTSIFRDQITRIDLQTIV
                     VNPTIAFKINEFLSVGAGIDFMYGRAKLDQTGVVRLGAAPLDQVNIFQLDLDGDGTAW
                     GYNFGLLMKPTENLRIGANYRSPFNLTIKDGDVKLNNINSTVGFVPPGRFSAAQVFGG
                     TSFNTRASTTLNMPATFALGASYTMGKVTVNADADWTLWSSFKSLDIDFQKNTGLLPD
                     VSRPERWKDVVAIRVGGEYRVTDPLALRLGFVYDPSPVPADTLGPLLPDSNRFFYAAG
                     AGYKVGAWTIDLAYMYLDKKDRTVNNQVNLAPPKTGSGFNGTWTGDAHLVSLDVGYKF
                     "
     gene            complement(8923..>9410)
                     /locus_tag="VF357_05935"
     CDS             complement(8923..>9410)
                     /locus_tag="VF357_05935"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HEX7523720.1"
                     /translation="SRLTAEFAVPFGGDAIGLHLVKALVLRGGGERFEARFRLENRSA
                     ETVSGWFCSEWNLNMLSGNGPDRYYEGMGEARALSSSGVAKGVRGFRVVDAWRNVAAG
                     AALDRECVVLREPVETASLSESGAEKIHQGVCLRILFPVRLEPGNHERYSILWSFKSV
                     D"
BASE COUNT         1599 a         3314 c         3036 g         1461 t
ORIGIN      
        1 tcaccttgta ttcgaccgtg gtcatgcgcg aagtaagggg aagggcgctt cgcagcgccg
       61 tcgccacgga catgtcgacg agcgttccca gcacaccgcc gtgaagcgtt cctcccgaat
      121 tcttgagcgc ctgcctcacg cggcactgca tcacgctcgt gccgccgctg cacgaaatca
      181 gcttcattcc catcatcttc atgaacggga accggttcac ccggtccttg accagatcct
      241 cgtcgaacgt cttcatcccg tcctccgccc cctcatccct tgtcgtaatg cgagaacttc
      301 atcgtatagg tcccgcgccc ctgggacagg gagcgcaaag cggtgacgta gccgaacatc
      361 tccttgagcg gaatcgccgc cgacaggaag ctcccgccgg ggcgccggtc gaccgaggaa
      421 accttgccgc gcctggcgtt gacgtcgccg atcaccccgc cgaggaactc gtcgggcacg
      481 acgatctcga ccgccatcac cggttcgagc aggtacggct tccccttctc gtacgcctcg
      541 tggaacgcct tggcggccgc caccttcgcc gccatcggcg acgggacgcc ggagaggaac
      601 tcgaggcggg agagctcgat cgtcgcatcg tccaccggat aaccgaggga gccgaagaac
      661 gccccttccc gcagccccat ctccgcgccg tcggctatgt ccggcggcag gccgagcagg
      721 cggaacccgt cggagatccg aacaccggag ccgcgttggg cgggcgacac ggccaccacg
      781 gtcaccacct tcacctggcg ttccgcgatc tcccgctcga agacgctctc cgcgcgcccc
      841 gccgacgcaa ccgtctcccg gtagagcacc tgcgggttcc ccttgcgcac ctccatgccg
      901 tgctcccgcg ccaggcgatc gatcgcgatc tccaggtgga gctcccccat cccggagagg
      961 aggatctgcc ccgtgtcggc gtcctcgcgg aacgacagcg tcgggtcctc gtccgtcatc
     1021 gcgccgagga gctcccgaag gcggtccatc tcccgcaccg tcctcggctc gacgacgacc
     1081 gacatgaccg gcttgcggat ctcgatcgac tcgtacacga tcggcgcccc ggggtcgcac
     1141 agcgtgtccc ccgtgcgggc gaaccggatc ccccgcgcgg cgacgatgtc ccccgccgcc
     1201 gcgacggcga tccgctcctt cttccccgcg tgcatccgga acagacgggc caccttctcc
     1261 tcctgttcgg tggcggcgtt cagcaacgtg tccccttcct tcgccttccc cgagtagacg
     1321 cggagataga cggtgcggcg cccttcctcc tggaggacct tgaacacgag ggccgagaac
     1381 ggggccgacg ggaccggctc gcgcgtcgcg ggcacgccgg tccgggggtc gtcgccccgg
     1441 gcgggccggg cctcctccgg agaggggaga taaaggacaa tcccgtccat cgccggctgc
     1501 accccgcggt tgcgcagcgc ggagccggcg aagacgggga agatcttccc ggccagcgtc
     1561 cccttgcgca gggcggcccg cagcaacggc accgggaccg tctcccctgc gaggtacttt
     1621 tccgccgccg cgtcgtcgac gtcggccgcc gcctcgagca gcgcctcgcg gtaccgcgcg
     1681 cacgacaaac cttccgcttc ggagaggggc gcccgcgaca cccccgcccc caggtcctcc
     1741 gcggagaacg aaaccttctc catcgtcacg aggtcggcca ccgccacgaa cgtcccgttc
     1801 tcgcagagcg gaacggtcag cggaacgccg cgggcgtcga gcttgcgcgc catgtcgtcg
     1861 accacccggt cgaagtcggc cccgggccgg tcgagcttgt tgacgaacgc cagccgcggg
     1921 atcccgtgcc ggtcggcctg gcgccagacg acctccgact gcggctccac gccccccacg
     1981 ccgcaaaaaa ccgccaccgc gccgtccagc acgcggagcg accgctccac ctcgatggtg
     2041 aaatcgacgt gcccgggcgt gtcgatcagg tgaacctcgg cccccatcca cgagaagtgc
     2101 gtcgccgccg cggtgatcgt gatcccccgc tccctctcct gcggcaggta atccatctgc
     2161 gagtcgccgt cgtggacctc ccccatccgg tgggtgatcc cggcgtagta gaggagccgc
     2221 tcggtgaagg tcgtcttccc ggcgtcgatg tgcgcgatga tcccgatgtt gcggagattc
     2281 acggcggacg ccacgtcaga agttccgccg gaggaggttg aaggtcgacg tcccggggat
     2341 ctcgcggtcg gcggacagcg ccgacagcac ttccgtgatc tccgccggtc cgagcccccg
     2401 gaggtcggcg aagaaatccc ggatccccgc ctccctgagc acgtggagcg acccggacgc
     2461 ggagaacggc tccgcgggca gaatgatcga accgtgctcg tccgccgtca cgcggaaccg
     2521 ctcggcgcgc gggctctcga cctcgccggc gcgcacccct gccgccggga ggagacggga
     2581 gatcatgagc ggcaccgggc cgtacgcgac ggcgatcccc agcccgtaga ggaatttccc
     2641 cacgtcccgc aacgccggga cggtcgcctc cacgggaagg atcatccgcg tcgcccccat
     2701 ccgcgaaaga gccgcgaggg cggccatgtt gaaggcgtag aggtaatggt cgctgatcaa
     2761 cgtgacctgg cgccgcaggg gagcgggtgc gaagaggcga aagtgcccgg catcggacac
     2821 gacccatcgg gtgaacccct tgcggatcgc gtcggtcacg gtacggcgaa ggaacgccgc
     2881 gtccgactcg agcatcggcg aggggagccg cagaaacccg ccggatcggg cgtatcgcgc
     2941 cgccggggac ggatcgcgcg cgagggagcg ggtgaactcc accacgggga cgacctccgg
     3001 cgtcttcggg aggtgcggga gctgctccgg gcggcacccc gcgaagatca ccgtgccggg
     3061 cccctcttcc ggccggctcc ccgaaacccg cagcgtcggg aggatctcca cccgcagccg
     3121 cttcccggcg aggtagaact cccggtcgaa ctgccgggcc gcctggagga agagggtccg
     3181 cacgtccccc cacacgcccg gacctccgtg gatctcgacc cggacgcccg ccagtggaag
     3241 gtcgccccgg tacgcgtcgg caagctggcg ttccccatcg agggggaccg taccgggagg
     3301 gccgccgggc ggacccgaga tgcggtacac gaacaccttc tcctcgtttc cgtacgacgc
     3361 cttgaccgtc accgtccccg gagagacgga aaccacgaag cgggccccgt ccgacggcgt
     3421 cgtctccatc tccttgcggg caagccgggt gaactccgcg cggcccgttc ccccgacccg
     3481 gaacagcaga tcgccgggcg agacgggaaa cggcaccttg acccggagcc cgccttcctc
     3541 gcaccgcggg tcgatcgcgg agaagccccg ccccgccccg tcctcccgga actgcgcgcg
     3601 cagccggtcc ccgggagaaa ttcccgccgc gcccgggacg aacgcccagc cgtcctcgac
     3661 gcgcgccacc gtaccgacca gttccccgac gttccccgat tcgcctcccg tcgccacctc
     3721 gcccgggcgc gcgcccccgg ccagcccggg ggtcgtctcc cgtccgatga cctgcgagag
     3781 gatccgcgtc ccctcggcca ccccctccac cgggttcccc gcgcgaatcc cgtcgagggc
     3841 cgcccggtac gccgacacga cgccggccac gtactccgcc ccccgcatcc gcccctcgat
     3901 cttcagcgag gcgatcccga gggggacgag gtccgggaga tgcgggagaa gggacaggtc
     3961 gcgggtggag aagatcgctt ccggctcccc cccgtggccg tacaggcgcc ggcacggctg
     4021 gacgcagacc ccgcggtttc cgctcttgcc gccgaggtac gaggagaaga agcacttgcc
     4081 ggagtaggag tagcacatcg acccgtggac gaagatctcc acgcctgcgg gagagcgctc
     4141 cgcgatccgg gcaatctctt ccttgcggag gtggcgttcg aggatgaccc gggacgcccc
     4201 catccgggcg aactcctccg ccgcctccgc ggaggcgcag ccggcctgcg tgctcatgtg
     4261 cacgggaatc cccgggaaga attcgtggag gatccgcacc aacccgagat ccgccgcgat
     4321 gatcgcgtcg ggctgaagcg gcgccacctg gtggagcagg ccgatcgcct cgggaaggtc
     4381 cgcctcggtg aggagggtgt tcatcgccac gtacagccgg acgccgcgtg cgcgggcgtg
     4441 gggccgtatg cggcaaagat ccgcgaggtt gaagttctcc gcccgctcgc gcgcgttgaa
     4501 cttcccgagg cccagataga cggcatcggc ccccgcgtcg acggcggcat agtacgcctc
     4561 cgcggacccc gcgggcgcga ggagctccgg gaacgcggga cgcggggcgc gggggagcgg
     4621 acccctcggg ccgaccggag gccgacgcgc ccccttcccc ggctcggcgg cgggccccct
     4681 cttcctcccc ttctcctgcg gcgcgggacg gggacgaggc cgtcccccgg aagatcccgc
     4741 tcgggcatcc cccggacggg gcgggagctt tacctccccg ggcccccgcc ggacgacgcg
     4801 ggatttcccc tcgctgccgc cggtcgggtg tttcgggtct tttccgtgcg gtgtgctgat
     4861 aagccgttcc ccttgggaat agttgcgagg tccttatttg gttgaaaacc atcatacatc
     4921 ggataaacta ttcggaaaat agcgatacgc aaggtgaatc gccccatttc ccccgccgga
     4981 gggacgtcga tggcgtacat tccgctggta ctgctggtgg tcatcgtcgc ctggttcgtg
     5041 gccgcctaca acaagctcgt tcgctatcgc aaccagatca agaacgcctg gcatcagatc
     5101 gacgtgcaac tgaagcgccg gtacgacctc atcccgaacc tggtggaggt cgtgaaggat
     5161 tacatggcgt acgaacagga gacgctcacc aaggtgatcg aggcccgcgg agccgccctg
     5221 acggcaaagg ggcccgcggc ccaggcgaag gcggaagggc tcctgaccga gacgctgaag
     5281 agcctcttcg ccgtcgtcga gaagtacccg gagctcaagg cgaaccagaa cgtcgcctcc
     5341 ctgcaggagg agctgaccgg gacggagaac aagatctcct tcgcccggca gttctacaac
     5401 gactcggtga tgacgtacaa caacgcgatc cagtcgatcc cgacaaactt catcgcctcc
     5461 ttcttcagct tcgcccagga agcgtacttc gaaacggaac ccgaaagccg ggcggtgccg
     5521 aaggcgagcc tgcggtagat tcgggagcgc ggcgtgatca gcggcatcgc cccggcgtgc
     5581 ccgaagtgcg gcggcgccaa ccgtcccggg gcggacctct gctacctctg cggggaatcg
     5641 ctcgaggcgg gtcccggcgc gaacgccttc gtcggcgacg cctcgcccct cgcggaggcc
     5701 cgtcctccgt tgcggccgat ccccatgctg ctcttcaccg aagcggcgcg gcacaacgtc
     5761 cggatgtccg ccctcctctt cgtccttctc ttcgccgtct tcttcctcct cgggatcgcg
     5821 atcgggggcg cctacgggga cccgcggacg gggctcggtc tctccttcgt gctgtacgcg
     5881 atcctcggga cgacggcgta cttcagcggc tcctcgatcg tgttgtcgat ccacggggcg
     5941 cgggaagcgg gcggggaaga gcatcggcag ctgcgcaacg tcgtcgagga gatggcgatc
     6001 gcggccggcg tgccgctccc caaggcatac gtgattcccg ccgcggggat gaacgccttc
     6061 gccgccgggc ggcgcccgga agaggcatgc gtcgccgtca cgaccggact tctcgaccgg
     6121 ctgaaccggg aggagctcca gggggtgatc gcgcacgagc tcgcgcacat caagagccgg
     6181 gatacgctgt acaacgtctg cgccgccgtc ctcgtcgggg ccgtcgccct cctgtccgac
     6241 atgttcctcc ggggcgcctt ctgggggcgc cgggggcgca cggaagggga acgaggaggc
     6301 ggcggccggg ggaacgccgc cttcttcgtc ctcgcgcttc tgctggcggt cctcgcaccg
     6361 ctggcggcga agatcctgca gatgagcatc tcccgacagc gggagttcca cgccgacgcg
     6421 gcggcggcag gcttcacccg caacccgctc ggcctggcct ccgccctcgc gaagatcacg
     6481 gccggggggc cggaagtccc cggtgagaac cgcgggacgc agcacctgtt catcgtgaac
     6541 ccgctgcggg tgttcgggcc ggacgactcc gcgctgatgt cgacgcatcc ccccaccgag
     6601 gagcgcatcc aacgattgcg agccatgggc ggggtcgccg ccggaaactg atgccgcaag
     6661 gagggggaag atgccgcgcg tcgtcaccat cttcgggaag gacacctgac cgtacaccac
     6721 caacgctcgt agggactacg agaaggccgg cgctgccgtg gagtaccgga acgtcctgaa
     6781 ggatcccgcc tcgttgtccg agatgctgcg gctcgtgggc ggccgcaggg atgttccggt
     6841 cgtactggag ggtgggaagg cgaccgtcgg ctacggcgga agttgaggtg tctgacgggt
     6901 cggccggtgg ccgaccgaag ccggagtgtg gtagaaagga tttccatcca accacggagg
     6961 acgcaaacca tgcaattttc cgaagacgcg ctgaagttcc tcaaccttgg aatcaactcc
     7021 gagatcgcgg cgtatgtctt ctacaagcac gccggcgcca tcgtgacgga gaaagggctc
     7081 caggataccc tccacaggct ggccaacgac gagaaggggc acttcctgtc gctggaagac
     7141 atctacgacc ggaacgtccg gtccgagatg tgggcaccct acaaggacat cctcagcaag
     7201 gcggggcttc cggacatcga cgagctcgtg caggacacgc acaaggagct gctctcccgg
     7261 atccggggga tcgcgacgaa gcgtgaggtg ctggagatgg cgctcgtgct ggagaaggag
     7321 gcgttcactc tcttcagcga gggagcggcg aagatgaagg acccggaaat caagaaggtg
     7381 ttcgacttcc tcacggggtt cgagcgggtc cacgtgatgc tgatcgagaa ggagctggcc
     7441 acgctctgat cgcatgcggg gagggacctc ccggcccctc cccgctgcgc gtcaacttag
     7501 aacttgtacc cgacgtccag ggacacgagg tgggcgtccc cggtccaggt cccgttgaag
     7561 ccggacccgg tcttgggagg cgccaggttc acctggttgt tcaccgttct gtccttcttg
     7621 tccaagtaca tgtacgccag gtcgatcgtc cacgcgccga ccttgtaccc ggcgccggca
     7681 gcatagaaga agcggttgga gtccggaagc aatgggccga gcgtatccgc cggaaccggt
     7741 gaggggtcgt agacgaaccc caaccgaagc gccagcgggt cggtcacccg gtattccccg
     7801 ccgacccgaa tcgccacgac atctttccaa cgctccgggc gggagacgtc gggcagcaag
     7861 ccggtgttct tctggaaatc gatatccagc gacttgaagg aggaccataa ggtccagtct
     7921 gcgtcggcgt ttaccgtcac cttccccatc gtgtacgagg ccccgagggc aaacgtggcc
     7981 ggcatattaa gcgtggtgct tgccctcgtg ttgaacgagg tcccaccgaa gacctgcgcc
     8041 gcggaaaatc tccccggcgg aacaaaaccc actgtgctgt tgatgttgtt gagcttcacg
     8101 tctccgtcct tgatcgtgag gttgaacggg ctgcggtaat tagcgcctat ccggagattc
     8161 tccgtgggct tcatcagcaa cccgaagttg tacccccacg ccgtcccgtc gccatccagg
     8221 tcgagctgaa agatgtttac ctggtccaga ggtgccgccc caagtctcac cacacccgtc
     8281 tggtcaagct tcgccctccc atacatgaaa tcgatcccgg cgcccacgga aaggaactcg
     8341 ttgatcttga acgcgatcgt cgggttgacg acgatcgtct ggaggtcgat cctcgtgatc
     8401 tggtcacgga agatgctggt ggtcttgctg ttgtactcct gcccaaggcc gaacggcgag
     8461 aagatgccga cgccataggc aaaatacccg tcggaggtgg ttttcgtgta atacaggttc
     8521 ggaatgaaga aatccaggct cttctgcgtc tcggatttgc tgtcgaacga gccggtcccc
     8581 gtattcaggg tgagaggagt ggtccccgtg aacgtcgcgc cgttctcgcg gatatacgtc
     8641 acgccgacca tgacgttctg ccccttgagc tgcgtgagcc ccgcggggtt gaagtagatc
     8701 gcggacgggt cgtccgcctg ccccgtgaac gcgaacccca ttcccatcgc cttcgccccg
     8761 gcctccggga gccggaaccc ggcggcgaac gacgtcgacg cggtgaacag caggaccagc
     8821 aacaccggta acatcttcca tcccttcatg cgcaccctcc ttgcgggtgt ggcccgcccc
     8881 cagtggaatt cgtgtggtcc caacataccg ttttatgatt cgtcaatcaa cggatttgaa
     8941 cgaccaaaga attgaatatc gttcatggtt tccgggctcc agccggacgg ggaagaggat
     9001 ccgcaggcag acgccctggt ggatcttctc cgcacccgac tcggaaaggg atgccgtttc
     9061 caccggctcc cgcagcacga cgcactcccg gtcgagggcc gcccccgcgg ccacgtttcg
     9121 ccaggcatcc acgacgcgga aaccgcgcac ccccttcgcg accccggaag aggaaagcgc
     9181 gcgcgcctcg cccattcctt cgtaataccg gtccggcccg ttccccgaca gcatgttcag
     9241 gttccactcc gagcagaacc accccgacac cgtctccgcg ctccggtttt cgagccggaa
     9301 acgcgcctcg aatcgctccc cgcccccgcg aagaaccagt gccttcacca ggtggagacc
     9361 gatcgcatcc cctccgaaag ggaccgcgaa ctccgcggtg aggcggctcc
//
LOCUS       DASVJL010000281         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1546526, whole genome shotgun sequence.
ACCESSION   DASVJL010000281 DASVJL010000000
VERSION     DASVJL010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546526"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VF357_05940"
     CDS             complement(<1..839)
                     /locus_tag="VF357_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7523721.1"
                     /translation="MILVLGGGITGLAAARTLAAAGEEFLVLEAEAEPGGWCRSIRSG
                     RYVFDRSGHFLHLSDPRMREWVLDLPGVLWETVARDARVLLRGKITPYPFQANLYGHA
                     PDVIRRCLTDFAAERIRETVSGPVEGKNFEEWLLRRFGRAMCREFFFPYNRKMWCTSL
                     RGMGYGWTSWSVPVPGIADLLAGARGETRTGMGYNPSFLYPRSGGIGALVSALSTGVR
                     GRVRAATRISRLDLRKKVAWSAGEEAFHFDAVLSTIPLPEMARSCVNLPSGVRAAASA
                     LRWV"
     gene            complement(850..1866)
                     /locus_tag="VF357_05945"
     CDS             complement(850..1866)
                     /locus_tag="VF357_05945"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX7523722.1"
                     /translation="MMLTGAGFLAAAASCLLSLLLALYLTPIFRDAALRFGIVDAPDG
                     KLKTQREPVPYLGGVAVFAAILFPVSLFLQFSDKVSGLLLACSIIVLLGLIDDIGRLS
                     PRVKLLVQVTAVFLMMKSGIRIRIEAFPPLLCVALTFLWMIVMTNGFNLIDVMDGLSA
                     GVATVSALALAVVFVVQGNRMGLILCLSLVGALLGFLRYNRPPAQIYLGDTGSLLIGF
                     LLGGLAIEGEYTARNPYGWLTALAVFAVPLFEIVFVSWLRFRRGASIFAGSRDHFSLR
                     LRRWKLTRGQTVSASCGAAAVAAAVGLLGMGLAPLPSLFAYGSIGLLFLLVAVWLKTI
                     DMGL"
     gene            complement(1863..2633)
                     /locus_tag="VF357_05950"
     CDS             complement(1863..2633)
                     /locus_tag="VF357_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7523723.1"
                     /translation="MGNRIFSVAANTFRETIRNKILYAILGFALLVIGLSYFLAGLSV
                     GEMTRIIANVGLASIHVFGVIMAVFIGITLISQEVERKTVYLILSRPVPRWEFIVGKA
                     LGLSLTLALTTLVMSITLFLVHAGYGREPETGILIASGGIYMELVLLICLASLFSTFT
                     TPVLSAMFTLSVFLIGHVSNDLLVFGGRADSGAVRWVSKVLFYALPNLEYFNWKNGVV
                     YGVVRSPAILLPATGYLLCYGGAVLLLACLLFSRKDFK"
     gene            complement(2623..>2846)
                     /locus_tag="VF357_05955"
     CDS             complement(2623..>2846)
                     /locus_tag="VF357_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX7523724.1"
                     /translation="SPPPAQQWVSSVGLPPGSGMYQGDTFVLKAGDEEEANRWIEEFR
                     RAGSLVRSCVPVKKHLEEIYVERVGGHGE"
BASE COUNT          654 a          906 c          885 g          401 t
ORIGIN      
        1 acccaccgca gggccgaggc ggccgcccga acgccggatg ggaggttcac gcaggagcgg
       61 gccatctccg gcaatggaat cgtcgaaagg actgcgtcga agtggaacgc ctcctctccg
      121 gcagaccagg ccaccttctt ccgcaggtcg agccgcgaaa tccgggtcgc ggcgcgcacc
      181 cggccgcgga cacccgtcga aagggcggag acaagagcgc cgatgccgcc ggaacgggga
      241 tagaggaagg acgggttgta tcccatcccc gtccttgtct cgccgcgggc gcctgcgagc
      301 agatccgcga tccccggcac ggggaccgac caggaggtcc agccgtaccc catcccgcga
      361 agggaggtgc accacatctt ccggttgtag gggaagaaga actcccggca catcgctctc
      421 ccgaatctcc ggagaagcca ctcctcgaaa ttcttccctt cgaccgggcc gctcacggtc
      481 tcccggatcc gctcggcggc gaaatcggtg aggcaccgcc ggatgacgtc cggtgcatgc
      541 ccgtacaagt tggcttgaaa cgggtacggg gtgatcttcc cccggagcaa tacccgggca
      601 tcgcgcgcga cggtctccca caaaacaccc ggaagatcga ggacccactc ccgcatgcgc
      661 ggatcggaaa ggtggaggaa gtgaccgctc cggtcgaaca cgtaccggcc ggaccggatt
      721 gaacgacacc atccgccggg ctcggcctct gcctcgagga ccaggaactc ctccccggcg
      781 gccgccagcg ttctcgccgc tgccagcccg gtgatccctc ccccgagaac gagaatcatc
      841 cctgcacctt cacagtccca tgtcgatcgt tttcaaccat accgcaacca ggagaaacag
      901 gagtccgatg gatccgtaag caaacaggga cgggagagga gccaatccca ttcccagcag
      961 ccccacagcc gcagcaacgg cggcggcccc gcaactggcg gaaaccgtct gcccacgggt
     1021 cagcttccaa cgacggagtc ggaggctgaa gtgatcccgg ctgccggcga agatggaggc
     1081 accccggcgg aatcgcagcc aggagacgaa gacgatctcg aacaggggga cggcgaagac
     1141 ggccagcgcg gtaagccagc cgtaagggtt cctcgccgtg tactccccct cgatggcaag
     1201 cccgccaagg aggaaaccga tcaggagcga gccggtgtcc ccgaggtaga tctgcgccgg
     1261 gggccggttg taccggagga agccgaggag ggcgccgacc agggagaggc agaggatgag
     1321 tcccatccgg ttcccctgca cgacaaacac aacggccagc gccagggcgg agaccgtcgc
     1381 cacacccgcg gaaaggccgt ccatcacgtc gatcaggttg aagccgttcg tcatcacgat
     1441 catccacaga aaggtgaggg cgacgcacag gagcggagga aacgcctcga tgcggatccg
     1501 gattccggat ttcatcatga ggaacacggc ggtcacctgg accagcagct tgacgcgggg
     1561 tgaaagccgg ccgatgtcgt cgatcagtcc gagcagaacg atgatggagc atgcgaggag
     1621 caacccggag accttgtcgc tgaattgcag gaacagggaa acggggaaga gaattgcagc
     1681 gaagactgcg acacccccga ggtaagggac cggctccctc tgtgtcttca acttcccgtc
     1741 cggggcgtcc acgataccga accgcagcgc cgcatcacgg aagatcggcg taaggtacag
     1801 ggccaggagg agggagagca ggcaggaggc ggcagcggca agaaatcccg cgccggtcag
     1861 catcatttga aatcctttcg cgagaagagc agacacgcca gcaagagtac cgcccccccg
     1921 tagcaaagaa ggtaccccgt cgcggggagc aggatcgcgg gcgagcggac taccccgtac
     1981 accaccccat tcttccagtt aaaatactcc agattcggga gcgcatagaa aaggaccttc
     2041 gatacccatc gcactgcacc ggaatccgcc cgcccgccga aaacaagaag atcgttcgat
     2101 acgtggccga tgaggaacac ggagagcgta aacattgcgc tcaacaccgg agtcgtgaag
     2161 gttgaaaaga gggaagcaag acagatgagc aggacgagct ccatgtaaat tccgccggac
     2221 gcaatcagaa tgcccgtttc cggctccctt ccgtatcctg cgtgcaccag gaacagcgtg
     2281 atggacatga cgagcgtcgt cagcgcgaga gtcaggctga gccccaatgc tttccccacg
     2341 atgaactccc accgcgggac cggcctggag aggatcaggt agaccgtctt ccgctcgact
     2401 tcctggctga tcaacgtgat cccgatgaac accgccatga tgacgccgaa gacatggatg
     2461 gatgccaacc cgacgtttgc gatgatccgg gtcatctccc ccacggacag gcctgcaagg
     2521 aaataggaga gcccgatgac gagaagggca aacccgagaa tggcataaag gatcttgttg
     2581 cggatcgtct cgcggaaggt gttcgcggcg acggagaaga tcctattccc catggccacc
     2641 tacgcgctcc acgtagattt cctcaagatg tttcttgacc ggaacgcacg agcgcaccaa
     2701 tgagcccgca cgacggaact cctcgatcca ccggttggct tcctcctcgt ccccggcctt
     2761 caggacgaag gtgtcccctt ggtacatgcc tgaccctggg ggcagcccca cggaggaaac
     2821 ccattgctgt gccggaggag gagaga
//
LOCUS       DASVJL010000282         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_982896, whole genome shotgun sequence.
ACCESSION   DASVJL010000282 DASVJL010000000
VERSION     DASVJL010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982896"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2014
                     /gene="acnA"
                     /locus_tag="VF357_05960"
     CDS             <1..2014
                     /gene="acnA"
                     /locus_tag="VF357_05960"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX7523725.1"
                     /translation="GIEAEAAICGQPLYMTAPPVVGMKIVGRLREGVTATDVTLTAVQ
                     ILRKKGVVEKFVEFYGPGLGQLSLPNRATLANMAPEYGATMGFFPVDQETLDYLGRTG
                     RRAGVELVERYTKEQGLFRTDATPDPEFTESLEFDLGTVEPSLAGPKRPQDRVPLAKM
                     KSAFAQALTAPVKERGFGLASEETSKKMSVQLNGKKTEIGHGAVVIAAITSCTNTSNP
                     SVMLGAGLLAKKAVARGLLVPAHVKTSLAPGSKVVTEYLKKTGLLTDLETLGFHVVGY
                     GCTTCIGNTGPLAEPVCTAVTEGKLVVAAVLSGNRNFEGRISPEVKANYLASPPLVVA
                     YALAGRTDLDLTTEPIGADRSGKPVFLSEIWPSSKEVADLEATVSGEMFAKTYGNVFD
                     GNPTWNAVGGAAGDLYEFKEVSTYLQDPPFFEGLTLDPKPLTDIRDARILVILGDSVT
                     TDHIAPAGDIAENSPAGRYFKARGVEKKDFNTYGARRGNDRVMARATFANIRLKNFMV
                     PGVEGGVTIHLPSGERMDVFDAAERYKKEGVPLVVIAGKEYGSGSSRDWAAKGPMLLG
                     VRAAIVESFERIHRSNLVGMGVLPLQFKPGQNAESLGLTGKERLTIQGISGKLTPRQD
                     VTVEVERADGTTTSFVTTARIDTPVEISYYRNGGILQTVLRKMLKQ"
     gene            complement(2131..>2591)
                     /locus_tag="VF357_05965"
     CDS             complement(2131..>2591)
                     /locus_tag="VF357_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX7523726.1"
                     /translation="YAAGMRATLPVKEKGYAEVLYLDCREKKYLDESGSSNFYAIIGK
                     RFATPASPSILPSITNMSVQQIAKDMDLKVEQRPVSVDELSSFDEAGCLGTAAVITPV
                     EAITYRGKDFVYVKDGKAGPITMEMYKRLTAIQLGEAPDPYGWTEIISEG"
BASE COUNT          472 a          793 c          891 g          435 t
ORIGIN      
        1 cgggatcgag gcggaggcgg ccatctgcgg tcagccgctc tacatgacgg cgcccccggt
       61 cgtggggatg aagatcgtcg gccggctccg cgagggtgtc acggcgacgg atgtgaccct
      121 gaccgcggtc cagatccttc gaaagaaggg ggtggtcgag aagttcgtag aattctacgg
      181 gccgggactc ggccaactga gcctccccaa tcgcgccacg ctggccaaca tggcgcccga
      241 atacggggcc acgatgggct tctttccggt cgatcaggag acgctcgact atcttggccg
      301 cacgggtcgt cgggccgggg tggagctggt ggagcgctac accaaggagc aaggcctctt
      361 ccgcacggac gccaccccgg atccggagtt caccgaaagc ctggagttcg acctcgggac
      421 ggtggagccg agcctggcgg gcccgaagcg ccctcaggac cgcgtacccc tggccaagat
      481 gaagtctgcg ttcgctcagg cgctcaccgc gccggtgaag gagcgaggct tcggtcttgc
      541 ctcggaggag acctccaaga agatgtcggt ccagctcaat gggaagaaga cggagatcgg
      601 ccacggcgcc gtggtcatcg cggccatcac cagttgcacc aacacctcca acccgtcggt
      661 catgctgggg gcgggcctcc tggccaagaa ggcggtggcg cgcggcctct tggtgccggc
      721 ccacgtgaag accagcctgg ccccgggctc caaggtcgtg accgagtatc tgaagaagac
      781 ggggcttctc acggatctgg agaccctggg attccatgtg gtgggttacg gctgcaccac
      841 gtgcatcggc aacaccggcc ccctggccga gccggtgtgc acggcggtga ccgagggaaa
      901 gctggtggtg gctgcggtgc tctcgggaaa ccggaacttc gaggggcgca tcagcccgga
      961 ggtcaaggcc aactacctgg cctcgccgcc gctggtcgtg gcgtatgccc tggcggggcg
     1021 gacggatttg gacctcacga cggagccgat cggggcggat cggagcggca agccggtgtt
     1081 cctgtcggaa atctggccta gctcaaagga ggtcgccgac ctcgaggcca cggtcagcgg
     1141 cgagatgttt gccaagacct acggaaacgt cttcgacggg aacccgacgt ggaacgccgt
     1201 cggcggcgcg gcgggggatc tctacgaatt caaggaggta tccacctacc tgcaggatcc
     1261 acccttcttc gagggcttga cgctcgatcc caagcccctc accgacatcc gggacgcccg
     1321 gatcctggtg atcctcggcg actccgtcac caccgaccac atcgcgccgg cgggggacat
     1381 cgccgagaac agcccggcgg gccgctactt caaggccaga ggggtggaga agaaggactt
     1441 caacacttac ggggcccgac gcgggaatga ccgggtcatg gcgcgtgcga ccttcgccaa
     1501 catccggctc aagaacttca tggtgccggg cgtggaaggc ggggtgacga tccatcttcc
     1561 ctccggtgag cggatggacg ttttcgacgc ggcggagcgg tacaagaagg aaggggtgcc
     1621 gctggtggtc atcgccggca aggagtacgg cagcggctcg tcccgggact gggcggcgaa
     1681 ggggccgatg ctcctggggg tgcgggccgc catcgtcgag agtttcgaga ggatccaccg
     1741 gagcaacctg gtggggatgg gcgtgttgcc cttgcagttt aagccgggcc agaacgccga
     1801 gtcgctcgga ctcaccggca aggagcggct gaccattcag gggatcagcg gaaagctcac
     1861 cccgcgacag gacgtcaccg tcgaggtgga gcgggcggat ggcactacga cctcgttcgt
     1921 taccacggcg cggatcgaca cgccggtcga gatcagttat taccgaaacg gcgggatctt
     1981 gcagacggtg ctaaggaaga tgctgaagca gtagcggacg cgctggaaga tctgcatgcc
     2041 gccgcttcgt gccggctatg aatcgaaagg ccctgaccga atcccggtca gggcctttcg
     2101 cgctgggatg gttcaggatc ttggaccgat tcacccctcg gaaatgatct ccgtccagcc
     2161 gtacggatcg ggggcctcgc ccaactggat ggcggtgagg cgcttgtaca tctccatcgt
     2221 gatcggaccg gccttgccgt ccttgacgta gacgaagtcc ttcccgcgat acgtgatcgc
     2281 ttccaccggt gtgatcacgg cggcggtgcc cagacaaccc gcctcgtcga agctggacag
     2341 ctcgtccacc gacaccggcc gctgctctac cttgaggtcc atgtccttgg cgatctgctg
     2401 gacgctcatg ttggtgatac tgggcaggat ggaggggctg gccggcgtgg cgaagcgctt
     2461 tccgatgatg gcgtagaagt tcgacgagcc cgactcgtcg agatacttct tctcccgaca
     2521 atccagataa aggacctcgg cgtagccctt ctctttgacg ggtaatgtcg cccgcattcc
     2581 cgcggcgtag t
//
LOCUS       DASVJL010000283         4606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1638562, whole genome shotgun sequence.
ACCESSION   DASVJL010000283 DASVJL010000000
VERSION     DASVJL010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638562"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VF357_05970"
     CDS             <1..660
                     /locus_tag="VF357_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7523727.1"
                     /translation="TCICMGASIGNAHGMEKALGPDGAGKVVAVIGDSTFLHSGITGL
                     MNVVYNHGISTVILLDNDITAMTGAQENPATGKTLHGEPTRRTDLPALCRALGVEHVY
                     TVNPHDMKQTEEVLRREVARPEPSVIITKAPCALLPEHRRQKRPVYEVIADLCTGCKA
                     CSRLGCPAIEWVPFTPEEAVAAGKKATQKGMARISPLLCDGCSQCPPLCKFQAILEKK
                     S"
     gene            657..1247
                     /locus_tag="VF357_05975"
     CDS             657..1247
                     /locus_tag="VF357_05975"
                     /inference="COORDINATES: protein motif:HMM:NF005322.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HEX7523728.1"
                     /translation="MSVPKGDVFLAGVGGQGTLLASEVLCDAFLLSGYDVKKSEVHGM
                     AQRGGSVTTHLRFGPKVFSPLIEPGKADLLIAFEKIEALRFAHYLRPGGAMVVNAQEI
                     LPPSVATGQERYPQDVTERLRAVTDRLHMVDALAAALSMREVRTVNIVLVGAASHFLP
                     LPEAAYEEAMKARLPEKLVAVNVQAFRAGRALLQPA"
     gene            1376..2449
                     /gene="had"
                     /locus_tag="VF357_05980"
     CDS             1376..2449
                     /gene="had"
                     /locus_tag="VF357_05980"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: protein motif:HMM:TIGR03201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HEX7523729.1"
                     /translation="MANSPHRWWMTAPGEPMVKADFDPFPPSAGEVVVEEAGCGVCHT
                     DLGFYYDGVRVKSPLPLTLGHEISGRVVAAGAGAETWQGKAVIVPSVIPCGECELCVS
                     GHGTICRRQQMPGNDIQGGFATHIRVPSRGLCLVDEKRLAAAGLQLADVSVVADAVTT
                     PYQAVVQAEVRPGDFAVVVGVGGVGGYAVQIARAFGATVAALDVDPQKLDAMAASGAA
                     LTVNVREVQGRDLKKAVQEFAKKNGCPDTRWKIFECSGTAAGQDTAFGLLNHGATLSV
                     VGFTMDKVELRLSNLMAFHARALGNWGCLTELYPQALDLVLDGKITLAPFVERHPLND
                     INEVFAAAHARKLTRRAILVPGR"
     gene            2452..3222
                     /locus_tag="VF357_05985"
     CDS             2452..3222
                     /locus_tag="VF357_05985"
                     /EC_number="4.2.1.100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexa-1,5-dienecarbonyl-CoA hydratase"
                     /protein_id="HEX7523730.1"
                     /translation="MGATPLHVELEKDGALLRLRLSRPKANIVDAAMIAALRAALDEH
                     LTGARLRAVLLEAEGPHFSFGASVEEHLPGSCAAMLRELHALILQLVGSPVPLLVAVR
                     GQCLGGGLEVAAAGHLIFAAPGATLGQPEIKLAVFAPAASCLLPERIGQSRAEDLLFS
                     GRGIGAEEAHRIGLVDALADDPSQAALAYFEEHYAPRSASSLRFAVRAARFDFVARVR
                     RKLAEVERLYLEELMATHDAVEGLAAFIAKRPPAWEDR"
     gene            3234..4055
                     /locus_tag="VF357_05990"
     CDS             3234..4055
                     /locus_tag="VF357_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX7523731.1"
                     /translation="MPVFVGLDIGSSRTKVAVIDADRRVLGHAVRKSGTDFSATAAAC
                     LDESLEMAGSPRDGIACAISTGYGRANVAFVTTQSKTEIGCLSRWGRDRFQEAITIID
                     IGGQDNKVIKLDQDGRRASFKMNRKCAAGTGAFLEEMSLRLDIPLEAMDGLARQSLEM
                     VKLGSYCTVFSATEVLENIRHGKKVPDIVKGLFYSVVKRVLEMDSHAGRVVMTGGVVA
                     HNPYIVEMAGEMVGQPVVLPDFPQLAGAIGAALYALDETDPLGIRNNIPAGETNG"
     gene            4048..>4606
                     /locus_tag="VF357_05995"
     CDS             4048..>4606
                     /locus_tag="VF357_05995"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEX7523732.1"
                     /translation="MAEEKKATRKKIQAADLMNKIMADYFYGLNEAATTGKRKVAWCT
                     SVGPAELLQAMGFAVHFPENHAAMLGATRMSTDLIPAANAIGYSPDICSYLTADIGAY
                     LKGITPLAKAYPGIQSVPKPDVLVYNTNQCRDVQDWMAWYSKKLGVPSIGVTSPKNVN
                     DVTEAHIDDVTRQIEALIPTLEGIAG"
BASE COUNT          795 a         1526 c         1547 g          738 t
ORIGIN      
        1 acctgcatct gcatgggcgc ctcgatcggg aacgcccacg ggatggagaa ggcgctgggg
       61 cccgacggcg ccgggaaggt cgtcgccgtc atcggcgact ccacgttcct ccactccggc
      121 atcaccggcc tcatgaacgt cgtctacaat cacggaattt cgacggtgat cctgctcgac
      181 aacgacatca ccgcgatgac gggcgcgcag gagaacccgg ccacggggaa gacgctccac
      241 ggagaaccga cccggcggac ggaccttccc gcgctttgcc gcgcgctcgg cgtggagcac
      301 gtctacacgg tgaacccgca cgacatgaag cagacggagg aggtgctgcg gcgggaagtg
      361 gctcgccccg agccgtcggt gatcatcacc aaggcgccgt gcgccctgct cccggaacat
      421 cgccggcaga aacgcccggt gtacgaggtg atcgcggatc tgtgcacggg gtgcaaggcg
      481 tgctcccggc tcgggtgccc ggcgatcgag tgggtcccct tcacccccga agaggcggtc
      541 gccgcgggga agaaggcgac acagaagggg atggcacgga tcagccccct gctgtgcgac
      601 gggtgcagcc agtgcccgcc gctgtgcaag ttccaggcga ttctggagaa aaaatcatga
      661 gcgttccgaa gggtgacgta tttctcgccg gcgtcggagg gcaggggacc cttcttgcct
      721 ccgaggtttt gtgcgacgcg ttcctccttt ccgggtacga cgtgaaaaaa agcgaggtcc
      781 acgggatggc gcagcgcggg ggatcggtca cgacccacct gcgcttcggc ccgaaggtat
      841 tctcgcctct catcgagccg gggaaggcgg atctgctgat cgccttcgag aagatcgagg
      901 cgctccggtt cgcccattac ctgcggcccg gcggtgcgat ggtggtgaac gcccaggaga
      961 tcctcccgcc gtcggtggcc accgggcagg agcggtatcc acaggacgtc acggagcgcc
     1021 tgcgggccgt cacggaccgg ctccacatgg tcgacgccct cgccgcggcg ttgtcgatgc
     1081 gcgaggtgcg tacggttaac atcgtgctgg tgggggccgc ctcccacttc ctgccgcttc
     1141 ccgaagccgc ctacgaggag gcgatgaagg cccgtctgcc ggagaagctc gtcgcggtga
     1201 acgtgcaggc gttccgcgcc ggacgggcgc tcctccagcc tgcctgaacc tggccgacac
     1261 tacataaaac ggcgtatttg aataccgttg acagttaaaa ggcattgagg tactatttta
     1321 ccggatctca gcgcaaggtg ctccgaattc cttacctttc cgaggaggaa gccctttggc
     1381 caactcaccg catcgctggt ggatgaccgc tccgggggaa ccgatggtga aggccgactt
     1441 cgacccgttt ccgccgtccg cgggcgaggt ggtggtcgaa gaggccggtt gcggggtttg
     1501 tcacacggac ctcggcttct attacgatgg cgtgcgagtg aagtcgcccc ttccgttgac
     1561 gctcggtcac gagatcagcg gccgggtcgt cgccgcgggg gcgggggcgg agacgtggca
     1621 ggggaaagcg gtgatcgtcc cctcggtcat tccttgcggc gaatgcgagc tgtgcgtctc
     1681 gggacacggc accatctgcc gccggcagca gatgccggga aacgatatcc aggggggctt
     1741 cgccacccac atccgcgttc cgtcacgggg cctctgcctc gtggacgaga agcggctcgc
     1801 ggcggccgga ctgcagttgg ccgacgtctc cgtggtggcc gacgcggtga cgaccccgta
     1861 ccaggcggtg gtgcaggcgg aggtgcggcc cggcgatttc gccgttgtgg tcggtgtcgg
     1921 cggagtgggc gggtacgccg tgcagatcgc gcgcgccttc ggcgcgaccg tggcggctct
     1981 cgatgtggac ccgcaaaaac tcgacgccat ggccgcgagc ggcgccgcgt tgaccgtcaa
     2041 cgtccgcgag gtccagggcc gcgacctcaa gaaggcggtc caagaattcg cgaaaaagaa
     2101 cggctgcccc gacacccgat ggaagatctt cgagtgctcc gggaccgccg ccgggcagga
     2161 caccgccttc ggactgctca accacggcgc gacgctgtcg gtcgtcggct tcaccatgga
     2221 caaggtggag ttgcgcctct cgaacctgat ggcctttcac gcccgggcgc tggggaactg
     2281 gggatgcctg accgagcttt acccgcaggc gctcgatctc gtgctggacg ggaagatcac
     2341 cctggctccc ttcgtcgaac gccatccgct gaacgacatc aacgaggtct tcgccgccgc
     2401 ccacgcgcgc aagctgaccc ggcgcgcgat cctcgtaccc gggagatgac gatgggcgca
     2461 acccccctcc atgtggaact ggaaaaggac ggcgccctcc tgcgcctgcg tctctcccga
     2521 cccaaggcga acatcgtcga cgccgccatg atcgcggcgc tgcgggcggc tctcgacgag
     2581 cacctgacgg gggcgcgcct gcgcgccgtt cttctggagg ccgagggacc gcacttcagc
     2641 ttcggcgcca gcgtcgagga acacctgccg ggatcgtgcg ccgcgatgct ccgcgaactg
     2701 cacgcgctga tcctgcagct cgtcggaagc ccggtccccc tcctggtggc ggttcgcggc
     2761 cagtgcctgg gcggcggcct cgaggtggcg gctgccggcc acctgatctt cgccgctccc
     2821 ggcgcgacgc tcggacaacc cgagatcaag ctggcggtgt tcgcccccgc ggcctcctgt
     2881 ctgctcccgg agcggatcgg ccagtcccgc gcggaggatc tccttttctc ggggcgcggc
     2941 atcggggcgg aggaagcgca ccggatcggt ctcgtggacg cgctggccga cgacccttcg
     3001 caggccgccc tcgcctattt cgaggagcac tacgctccgc gaagcgccag ttccctccgg
     3061 ttcgccgtgc gcgccgcccg tttcgacttc gttgcgcggg tccgccggaa gctcgccgag
     3121 gtcgaacggt tgtacctcga agagttgatg gccacccacg acgccgtgga agggttggcg
     3181 gccttcatcg cgaagcgccc accggcgtgg gaagaccgct gagaagaatg tcgatgcccg
     3241 tattcgtcgg tctcgacatc gggtcatcga ggaccaaggt cgccgtgatc gacgccgacc
     3301 gccgggtcct cggacatgcc gtccggaaat ccggaaccga cttttccgcc actgcggcgg
     3361 cttgcctcga tgaatcgctg gagatggccg gatccccccg cgacgggatc gcgtgcgcca
     3421 tttcgaccgg atacggccgg gccaacgtcg ccttcgtcac cacgcagagc aagacggaga
     3481 tcggctgcct ctcccgatgg ggccgcgatc gtttccaaga ggcgatcacc atcatcgaca
     3541 tcggcggcca ggacaacaag gtcatcaagc tcgatcagga cgggcgacgc gccagtttca
     3601 agatgaaccg gaagtgcgcc gccgggaccg gggcgttcct cgaggagatg tccctgcgcc
     3661 tcgacatccc cctggaggcg atggacggac tggcccgcca gtccctggag atggtcaagc
     3721 tcggaagcta ctgcaccgtc ttttcggcga cggaggtcct cgagaacatc cgccacggga
     3781 aaaaggtgcc cgatatcgtc aaggggctct tctactcggt cgtcaagaga gtgctcgaga
     3841 tggactccca cgccggtcgg gtcgtcatga cgggtggagt cgtcgcgcac aatccgtaca
     3901 tcgtcgaaat ggccggggag atggtcgggc agccggtcgt gctcccagac tttccgcaac
     3961 tggccggcgc gatcggcgcc gccctgtacg ccctcgatga aacggatccg ctcgggatcc
     4021 ggaacaacat ccccgcggga gagaccaatg gctgaagaga agaaggcgac gcgaaagaag
     4081 atccaggccg ccgacctgat gaacaagatc atggcggact acttctacgg gctgaacgag
     4141 gcggccacca ccgggaagcg aaaggtcgcg tggtgcacca gcgtgggccc ggcggaactg
     4201 ctccaggcaa tggggttcgc cgtccacttc ccggaaaacc acgccgccat gctcggcgcg
     4261 acgcggatgt cgaccgacct catcccggcg gcgaacgcga tcggctattc gcccgacatc
     4321 tgctcgtatc tcacggccga catcggcgcg tacctcaagg ggatcactcc gctcgccaag
     4381 gcgtacccgg ggatccagtc ggtgccgaag cccgatgtcc tggtctacaa cacgaaccag
     4441 tgccgcgacg tccaggactg gatggcctgg tactcgaaga aactcggggt cccctccatc
     4501 ggggtcacct ctcccaagaa cgtcaacgac gtgaccgagg cccacatcga cgacgtcacc
     4561 cggcagatcg aggccctgat ccccacgctc gaggggatcg ccgggg
//
LOCUS       DASVJL010000284         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1734253, whole genome shotgun sequence.
ACCESSION   DASVJL010000284 DASVJL010000000
VERSION     DASVJL010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734253"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VF357_06000"
     CDS             complement(<1..795)
                     /locus_tag="VF357_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523733.1"
                     /translation="MTEPPATPDDLYLQRLTQAVTDFAKGIKSASFYPAGHPVLLQAV
                     TKIILLFEAIPLPEEGLSIDVTKNALLYRDVPLPMGGNKALLDLNRELYLRRASRIIF
                     LPNLQPDEVVSCLKIITLDPEDIQDAGGLERILLQEKVTRIWANRVDYDQLTRLLKEE
                     ELEEVQPEELIAVPLSTSENPLQDNVPPEEAVTIETLITRIAKETDPSAYRGHIVEFS
                     RFLLAERAERKIEFSTQAMTIFIRHIEFPPGGSDEIAGLARLGIKEV"
     gene            901..1335
                     /locus_tag="VF357_06005"
     CDS             901..1335
                     /locus_tag="VF357_06005"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX7523734.1"
                     /translation="MRAVATGFGAGRFPVAPGTAGTLVALPLWYWSGGWGMRHLLLLC
                     VVLLVSIPAAREEMAATGSPDPGSVVIDEIAGMLLAATGIPWGIRPVLLLFLLFRVFD
                     VFKFGPAAWLDARRGAVYVVADDLAAGVCAGLAYRGIAWLIG"
     gene            1320..1829
                     /locus_tag="VF357_06010"
     CDS             1320..1829
                     /locus_tag="VF357_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEX7523735.1"
                     /translation="MADRLTASAARLLGAALSASGRTLAVAESCTGGLLGGAITSVPG
                     SSLYFSGGVVVYGNSAKVSLLGVPRDLIAARGAVSREVALAMAEGALSLFRTDLAIAV
                     TGVAGPGGGSRGKPVGTVWVAVVAPGGVRYAHRFRFPGGREAVRRETVRASLGAANDV
                     LRSETAEGE"
     gene            1826..>2310
                     /gene="thpR"
                     /locus_tag="VF357_06015"
     CDS             1826..>2310
                     /gene="thpR"
                     /locus_tag="VF357_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX7523736.1"
                     /translation="MRAFLGIGLPTGVREAIASATAEVRGLHDPVAWAAPANLHITLD
                     FLGEISPERVALVERSMRDVASGIGPFSLTAEGGGAFPGTRYPRVLWVGFLEPLELVR
                     QLQQNMENALSGVGFPREDRPFHPHITVGRTRGALPPAWGERFVQVLSGKGFGVVPVS
                     S"
BASE COUNT          322 a          696 c          825 g          467 t
ORIGIN      
        1 cacctccttg atcccgaggc gggcgagtcc ggcgatctcg tcgcttcccc cgggaggaaa
       61 ctctatgtgc cggatgaaga tcgtcatcgc ctgcgtcgag aattcgatct tccgctcggc
      121 gcgttccgcg aggaggaacc gggagaactc cacgatgtgc ccgcggtagg cggaggggtc
      181 ggtctccttc gcgatccgcg tgatcaaggt ttcgatcgtg acggcttcct cgggcggcac
      241 attgtcctgc agcgggttct ccgaggtcga cagcgggaca gcgataagtt cctctggctg
      301 aacctcctcg agttcctcct ccttcaggag tcgggtgagc tggtcgtagt ccacccggtt
      361 tgcccagatc cgcgtcactt tctcctgcag caggatccgc tccagccccc cggcgtcctg
      421 gatgtcctcc ggatccagcg tgatgatctt gagacaagag acgacctcgt ccggctggag
      481 gttgggaaga aagatgatcc tcgacgcgcg ccgcaggtac agctcgcggt tgaggtccag
      541 gagcgccttg tttcccccca tgggcagggg gacgtcccgg tagaggagtg cgttcttcgt
      601 gacgtcgatc gaaaggccct cttcgggcag ggggatcgcc tcgaacagca ggatgatctt
      661 tgttacggct tgtagcagga cagggtggcc ggcgggataa aagctcgcgc ttttgatccc
      721 cttggcgaaa tcggtgaccg cctgcgtgag ccgctgcagg taaaggtcgt ccggagttgc
      781 cggaggctcg gtcattccat cgttggaaaa tgaggtttta ttggcataga ctattaaata
      841 tccgacatgg gcgccgtaca agtcaatcgt tttcgggaaa accggtgggt tcgtggagga
      901 ttgcgcgccg tcgccacggg gttcggagcg ggccggtttc ccgtggcccc cgggacggcg
      961 ggtaccctgg tcgcgctgcc cctctggtac tggtcgggcg gatgggggat gcgtcatctt
     1021 ctccttctgt gcgtggtgct cctcgtttcc atcccggccg cccgggagga gatggccgcg
     1081 acagggagtc cggaccccgg ctccgtggtc atcgacgaga tcgcggggat gctgcttgcg
     1141 gccaccggca tcccctgggg gattcgcccc gtcctcctgc tcttcctgct gttccgtgtt
     1201 ttcgatgtgt tcaaattcgg gcctgcggca tggctcgacg cccgcagggg agccgtctac
     1261 gtggtggcgg acgacctggc ggcaggtgtg tgcgcgggac tcgcgtaccg ggggatcgct
     1321 tggctgatcg gctgaccgcc tctgcggcga ggctcctggg ggccgcgttg tccgcctccg
     1381 gaaggaccct cgccgtggcg gaatcgtgca cggggggcct gttgggggga gcgatcacct
     1441 cggttcccgg cagttccctc tatttttccg ggggcgtcgt ggtgtacggc aattccgcga
     1501 aggtctcgct gctgggcgtt ccgcgcgacc tgatcgccgc gcgcggagcg gtgagccggg
     1561 aggtggcgct tgcgatggcg gaaggggccc tctccctctt ccggacggac cttgcgatcg
     1621 ccgtcaccgg cgtggcgggg cccggcggcg ggagtcgcgg gaagccggtg gggacggtgt
     1681 gggtggccgt cgttgccccg ggcggggtgc ggtatgcgca ccggttccgg tttccaggag
     1741 gccgcgaggc ggtccgccgg gagaccgtcc gagcctcgtt gggcgccgca aacgacgtcc
     1801 tgcgatccga aacggcggag ggggaatgag agcattcctc ggcatcggtc ttcccaccgg
     1861 tgtccgggag gccatcgcgt ccgcgacggc agaggtccgt ggcctccacg accccgtcgc
     1921 gtgggcggca ccggcgaacc tgcacatcac cttggatttt ctgggagaaa tttctccgga
     1981 gcgggtcgcg ctcgtcgagc ggtcgatgcg ggacgtggct tccggaatcg gtccgttctc
     2041 cctgacggcg gagggagggg gagccttccc cggcacgaga tacccgcgag ttctctgggt
     2101 cggtttcctt gaaccgcttg aattggtaag gcagttgcaa cagaatatgg agaacgccct
     2161 atcgggggtc gggtttcccc gggaggaccg tccgttccac ccgcacatca cggtcgggcg
     2221 tacccgcggg gcgctccctc cagcgtgggg agaacggttt gtccaggtgc tttccgggaa
     2281 gggattcggc gtcgttcccg tgtcgtcgtt
//
LOCUS       DASVJL010000285         4746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1172484, whole genome shotgun sequence.
ACCESSION   DASVJL010000285 DASVJL010000000
VERSION     DASVJL010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172484"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VF357_06020"
     CDS             <1..613
                     /locus_tag="VF357_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7523737.1"
                     /translation="LLAAEKRFGNPIYVISDEPYRKIVFRGVSSTPAAAKIRNTMVAY
                     SHSKDLNLPGERIGYLAVSPRAADAAEVAGACVFCNRVLGFVNAPALFQLAASKFEDE
                     PADVSVYQENRDVLLDALRRAGIDVVPPGGAFYLFPKSPVPDEMAFVAAARDEGILVV
                     PGSGFGRSGHVRVAYCLSTETVRRSVPAWERLGRRYSAGGTRR"
     gene            610..1656
                     /gene="hisC"
                     /locus_tag="VF357_06025"
     CDS             610..1656
                     /gene="hisC"
                     /locus_tag="VF357_06025"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX7523738.1"
                     /translation="MTVVFRRNVSRMEGYVPGEQPRERDFIKLNTNENPYPPSPKVRM
                     AILRELGEPLRLYPDPGSTALRRQAALTYGFDLPGVIAGNGSDDLLAMIARAFVGEGD
                     LLACPVPTYTLYDTLVRIQGGKLSAVPYPDDYSLPRGLAGKKARVTIVANPNSPSGTA
                     VPVRALAELADAVPGLLVVDEAYADFADETALALARERRNVIVLRTLSKSFSLAGMRI
                     GLGFAHPRIIEGLDKVRDSYNLNRLSIAAGAAALTDTGWMERNAARIRKTRDALAAAL
                     PGVGFTPFPSQSNFILARRTRGGSARPVYEALKRRKILVRYFDTPRLAGCLRITVGTD
                     DEAGALLGALKALR"
     gene            complement(1666..2673)
                     /locus_tag="VF357_06030"
     CDS             complement(1666..2673)
                     /locus_tag="VF357_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523739.1"
                     /translation="MLLLRREYRDGAEPPEIVLLHAEVTRHGGPGPTAMDGLVPRQAG
                     MPPDLLPRPTRRFTRAVVPSDPPGRRVAHLPLPEPEEGERILVRYRFSTVRGGKERFS
                     PWYEVVVPSDEAIADLYHIPEEGASNLPPAPGRGHFRLVLPLGEGERASGLFRVGFGA
                     MRKKPSPSLCRAVIDAGYGPAPVIEAPEALSVLKNRPMPYFLYHVSADGNLHADKIAC
                     ARITFADPEGNVVSARMVWGDSAWRATNLSLMEAKGFTPSPGAAVEEFFAEDRAAFLA
                     AREAALSLLPLPRVFEAFVFGPSGSWVEYCFQTVARGPDGGFEVRWRNREGGGNWVVT
                     L"
     gene            complement(2720..3088)
                     /locus_tag="VF357_06035"
     CDS             complement(2720..3088)
                     /locus_tag="VF357_06035"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX7523740.1"
                     /translation="MRRDDFDRALREALLELPPMFRDALANVAVVVEEWAPDDLLDEL
                     GVPPDDTLYGFYHGVPLPERSVLDSGLLPDKISVYRGPLVEDFPDRSELCRQIRITLL
                     HEIGHYFGMDEEELSRLGYE"
     gene            complement(3110..3733)
                     /locus_tag="VF357_06040"
     CDS             complement(3110..3733)
                     /locus_tag="VF357_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523741.1"
                     /translation="MHLRIDGCDVSLELPEGSTFGAFLAGAGSKVASEGRVVSRIVAD
                     GREISTQFERDMADRPVGEIDTVEITTTTPDVLFREALDGAVDLSISIRRDVVTILSS
                     LRAGDLAMAGPRYAACIESLGTFFRLAGAVFNAIGTGMFPLAVRPGSAGELPTPPDAT
                     TGILQRLLRTQKGNDWNAVADILEKEILPNIDGWSAFFKALKEIGAE"
     gene            complement(3801..4004)
                     /locus_tag="VF357_06045"
     CDS             complement(3801..4004)
                     /locus_tag="VF357_06045"
                     /inference="COORDINATES: protein motif:HMM:NF016223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEX7523742.1"
                     /translation="MKVSDRGKWGALLDETRRVVAVLSEGRARRIEEIRSALRNGTYR
                     VDGRQVAGKMVSDAVRELRERPG"
     gene            4111..>4746
                     /locus_tag="VF357_06050"
     CDS             4111..>4746
                     /locus_tag="VF357_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7523743.1"
                     /translation="MKKSLLIAEDEEVTLALLRNVFGRSDLLVHEAHTGEEALQYIDQ
                     HPVDVILTDLKMPGADGLLVLAHARKVRPGAEVILMTGHASVESAVRAMKLGAFHYIT
                     KPFDIDEVAQLVDRALELASVRRENVSLRSLARGWGGLENFIGVSEATKEVLSLVRKV
                     ADTDSTILITGESGTGKELIARALHYLSPRSERMLVPINCSAIPAELLESEL"
BASE COUNT          787 a         1663 c         1574 g          722 t
ORIGIN      
        1 actgctcgcc gcggagaagc gattcgggaa cccgatctac gtgatctccg acgagccgta
       61 ccggaagatc gttttccggg gggtgtcgtc cacgccggcc gccgcgaaga tccgcaacac
      121 catggtggcc tattcgcact caaaggacct gaacctcccg ggcgagcgga tcggctacct
      181 cgccgtctcc ccgcgcgcgg cggacgcggc cgaggtggcg ggggcgtgcg tcttctgcaa
      241 ccgggtgctc gggttcgtca acgccccggc cctcttccag cttgccgcgt cgaagttcga
      301 ggacgagccc gccgacgtct cggtgtacca ggagaaccgg gacgttctgc tcgacgccct
      361 gcggcgcgcc gggatcgacg tcgtcccgcc cgggggagcg ttctacctgt tcccgaagtc
      421 ccccgtcccg gacgagatgg cgttcgttgc cgcggcgcgc gacgagggga tcctcgtcgt
      481 cccggggtcc gggttcgggc gaagcggcca tgtccgcgtc gcctactgcc tctccacgga
      541 aacggtgagg cgctcggtcc ccgcgtggga gcggctcgga cgccgctatt ccgccggtgg
      601 gacccggcga tgaccgtcgt cttccggcga aatgtctccc gcatggaggg atacgtcccc
      661 ggcgagcagc cccgggagcg cgacttcatc aagctgaaca ccaacgagaa cccgtacccc
      721 ccttcgccga aggtgcggat ggcgattctc cgggaactcg gggagccgct gcgcctctac
      781 cccgaccccg ggtccaccgc gttgcggcgc caggcggcgc tgacgtacgg cttcgacctg
      841 ccgggggtga tcgcggggaa cggctcggac gacctgctgg cgatgatcgc gagggcgttc
      901 gtgggcgagg gggatctcct cgcgtgcccc gtgcccacct atacgctcta cgacaccctc
      961 gtccgcatcc agggggggaa actctcggcg gtcccgtacc cggacgacta ctccctcccc
     1021 cggggtcttg ccgggaaaaa ggcgcgcgtg acgatcgtcg cgaatccgaa ttcgccgtcg
     1081 gggaccgccg tgccggtccg cgcgctcgcg gagcttgccg atgccgtccc cggcctgctg
     1141 gtcgtcgacg aggcgtacgc cgacttcgcg gatgagacgg ccctcgcgct cgcccgggaa
     1201 cggcggaacg tgatcgtcct gcgaacgctc tccaagtcgt tctccctggc ggggatgcgg
     1261 atcgggctcg ggttcgccca cccgcggatc atcgaggggc tggacaaggt gcgcgactcg
     1321 tataacctga accgcctgtc gatcgccgcg ggggcggcag cgctcacgga caccgggtgg
     1381 atggaacgga acgcggcgag gatccggaaa acccgcgacg ccctggcggc ggcccttccg
     1441 ggggtggggt tcacgccgtt cccgtcgcaa agcaacttca tcctcgcccg gcgcacgcgg
     1501 ggaggatcgg cccggccggt ctacgaggcg ctcaagcgcc ggaagatcct cgtgcggtac
     1561 ttcgacaccc cgcggctcgc ggggtgcctc cgcatcaccg tcgggacgga cgacgaggcc
     1621 ggggccctcc tgggcgcgct gaaggcgctc cggtaggcct cctcctcaaa gggtcacgac
     1681 ccagttcccc ccgccttccc ggttccgcca ccgcacctcg aagcctccgt ccggcccccg
     1741 cgcgacggtc tggaagcaat actccaccca gctccccgaa ggcccgaaca cgaacgcctc
     1801 gaagacacgc ggaagcggga ggagggataa ggcggcctcc ctcgccgcga gaaacgccgc
     1861 ccggtcttcc gcgaaaaact cctcgacagc ggcgccgggg gagggcgtaa accccttggc
     1921 ctccatgaga gagaggttcg tggcccgcca cgcggagtcg ccccagacca tccgggcgga
     1981 gacgacgttc ccttcgggat cggcgaaggt gattcgggcg cacgcgatct tgtcggcgtg
     2041 cagattcccg tccgcggaga cgtggtacag gaagtacggc atcggccggt tcttgaggac
     2101 agagagggcc tccggtgcct cgatgaccgg cgccggaccg tatcccgcgt cgatgacggc
     2161 gcggcaaagc gacggggacg gctttttccg catcgctccg aagccgaccc ggaaaaggcc
     2221 ggatgcgcgc tccccctccc ccaacggcag gaccaggcgg aaatgtccgc gccccggagc
     2281 gggcgggagg ttcgacgcgc cctcctcggg gatgtggtag agatccgcga tcgcttcgtc
     2341 cgagggaacg acgacctcgt accacggaga gaaccgttcc ttcccgccgc gaacggtgga
     2401 gaaccggtat cgcacgagga tccgctcccc ctcctccggc tcggggagag gaaggtgtgc
     2461 cacccgtcgg ccgggcgggt ccgaaggaac gaccgcccgg gtgaaccgtc gcgtcggccg
     2521 aggcaacaga tccgggggca tccctgcctg cctcggcact aggccatcca tggccgtcgg
     2581 cccgggacct ccgtgccgcg tcacctcggc gtgcagcagg acgatctccg gcggttcggc
     2641 gccgtcgcgg tactctcgtc gaagaaggag catcccgaac ctcctggtgc agcgtctcgc
     2701 aaatcccctg gacggagcct cactcgtacc cgaggcggga gagttcctct tcgtccatcc
     2761 cgaagtagtg gcctatctcg tgaagcagcg tgatccggat ctgtcggcac agctcgctcc
     2821 ggtccgggaa atcctccacg agcggccccc ggtaaacgga gatcttgtcg ggaaggaggc
     2881 cggaatccag gaccgaccgc tcggggagcg gaaccccgtg gtagaagccg tacaacgtgt
     2941 cgtccggagg gacacccagt tcgtcgagca ggtcgtccgg cgcccactcc tccacgacca
     3001 cggcgacgtt cgccagcgcg tcacggaaca tcggcgggag ttccaggagc gcctcccgga
     3061 gcgcccggtc gaaatcgtct cgccgcaccg tcccccctcc ccctggtgcc tactccgcgc
     3121 cgatctcctt gagcgccttg aaaaaggcgg accacccgtc gatgttgggc aggatctcct
     3181 tctcgagaat gtccgccacg gcgttccagt cgttcccctt ctgtgtccgg agaagccgtt
     3241 ggaggattcc ggtcgtcgcg tcgggagggg tcggaagctc cccggcgctt ccgggacgaa
     3301 cggcaagcgg gaacattccg gtcccgatgg cgttgaagac cgccccggcc agccggaaga
     3361 acgtcccgag ggattcgatg caggccgcat accggggacc cgccatcgcg aggtcccccg
     3421 cgcgcaggga cgacaggatc gttaccacgt cgcggcggat ggagatggag aggtcgacgg
     3481 cgccgtcgag tgcttcccgg aacagcacgt cgggggttgt cgtcgtaatc tccaccgtat
     3541 cgatctcccc aaccggccgg tcggccatat cgcgctcgaa ctgcgtcgag atctcccgcc
     3601 catccgcgac gatccgggag acgacccgac cctccgaggc gaccttgctc cccgccccgg
     3661 cgagaaaagc gccgaaggtc gatccctcgg gcagctccag ggaaacatcg cagccgtcga
     3721 tgcgcaagtg catggaaaaa cgatacgaca acgatgggtg gcaggcaatg gaaatcgggc
     3781 gggatggaaa gcggcgggga tcaacctggg cgttcgcgga gttcccggac ggcgtcggag
     3841 accatcttcc cggcgacctg tcttccgtcg acacggtacg ttccattccg gagggccgac
     3901 cggatctcct cgatccgtcg cgcccggcct tcggacagca cggcgaccac ccggcgcgtc
     3961 tcgtcgagca gcgcgcccca cttcccccga tcggaaacct tcaatgcccc tccgttccgc
     4021 cgccggcggc acgatgtccg cacagtatcc ttatcgacgg gcgggatcgg aaactttacc
     4081 cgggagggac gattctgtac actggaccgg atgaaaaaat ccctcctgat cgccgaggac
     4141 gaggaagtca cgctcgccct ccttcgaaac gtgttcgggc gctccgacct cctggtccac
     4201 gaggcccaca ccggagagga agcccttcag tacatcgatc agcaccccgt cgacgtgatc
     4261 ctcaccgacc tcaaaatgcc gggggcggac gggctcttgg tgctcgccca cgcccggaag
     4321 gtccgtcccg gggccgaggt gatcctcatg acggggcacg cgtcggtcga atccgccgtt
     4381 cgggcgatga aactgggcgc gttccactac atcaccaaac cgttcgacat cgacgaggtc
     4441 gcccagctcg tagaccgggc cctcgagctc gcgagcgtgc gcagggaaaa cgtgagtctc
     4501 cgttccctgg cccgtgggtg gggggggctg gaaaatttca tcggcgtgag cgaggcgacg
     4561 aaggaggtcc tctccctcgt ccggaaagtg gcggacaccg actccacgat cctgatcacg
     4621 ggggagagcg ggacggggaa agagctcatc gcgcgggccc ttcactacct ttccccgcgc
     4681 tccgagcgca tgctcgtccc catcaactgc tccgccatcc ccgccgaact gctcgagagc
     4741 gagctc
//
LOCUS       DASVJL010000286         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_515344, whole genome shotgun sequence.
ACCESSION   DASVJL010000286 DASVJL010000000
VERSION     DASVJL010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515344"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1435)
                     /locus_tag="VF357_06055"
     CDS             complement(146..1435)
                     /locus_tag="VF357_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7523744.1"
                     /translation="MTDPVALLSEYLRIDTSNPPGDCRGAAELLCGTLRQTGLSPVSF
                     GAKPEKPNVLCHVGGTEEPGLVLIHHMDVVPAKAEEWTVPPFSAQIRDGFLYGRGTLD
                     TKGLGVAHWCAALRAAKAGILRRKLFLVANADEEVGGGEGAEYFVRNLPFPIGAAYGM
                     NEGGVGVTGIFGGRGKFFLLNMWEKGPVWMKLSTAGRAGHGSRPSAKDAPARLARAMA
                     RVAEFREPARLSEPVRDMLRVLRAKGFSDLDVDALRGGAEEAGALEALGGRFPEIDPL
                     LRNTFAVTTLAAGFKPNVIPSSAEGTIDARIVPGEEPETVAARVRALVADLDVSVEIL
                     FAEKPNGSPMGPLYGAMEEAVLAVHPDAVVVPYLSTGFTDSRFFRSIGIPTYGLMPLL
                     LPRAEHGKIHGVDERIPVDGIEEMTRIVYALIERWNG"
     gene            1513..2553
                     /gene="gmd"
                     /locus_tag="VF357_06060"
     CDS             1513..2553
                     /gene="gmd"
                     /locus_tag="VF357_06060"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7523745.1"
                     /translation="MPKALITGVTGQDGSYLAEFLLSMGYEVHGIIRRASTFNTGRLD
                     HIYVDPHATGARMFLHYGDLSDGGQLTNLIYNVQPDEIYHLGAQSHVRVSFDIPEYTG
                     DITGLGTTRILESLRRAGGKARYYQASSSEMFGATPPPHGENTPFYPRSPYGAAKVYS
                     YWMAINYREAYNMFASNGILFNHESPRRGETFVTRKITRAIAKIKAGKQKDLFLGNLD
                     ARRDWGFAYEYVQVMWRILQHDRGDDFVIGTGQSNSVKEFLEEAFAYAGLDWKEYVKI
                     DPKYFRPTEVVNLIADASKAKKRLGWEPKVTFKELVRIMVDADLEDAGLPTPGEGRKI
                     LAARGLPVGSKL"
     gene            2557..3522
                     /locus_tag="VF357_06065"
     CDS             2557..3522
                     /locus_tag="VF357_06065"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7523746.1"
                     /translation="MSTFTGKKILVTGGSGFLGAPVIAQLLGRGARREDITVPRSARY
                     DLRLPGACAEVVPGQDLVIHLAANAGGIGWNRSHPGALFYDNAAMGIHLMEESRKAGV
                     KKFVQIGTVCAYPFQPPRIPFREDDLWEGYPEPTNAPYGIAKKAMMVMGHAYREEYGF
                     NVIYLLPVNLYGPRDHFFEPEKSHVIPALIQKFVDARESGAPEVAVWGSGFYRGTPVS
                     REFLYVDDAAEAIALAAERYDGAEPVNVGAGREVPVNDLVAMVGEMTGYRGKVVRDLT
                     KPDGQPRRCLDTSRAKERFGFVARTSFEEGLRKTIAWYEQARRER"
     gene            3586..>3820
                     /locus_tag="VF357_06070"
     CDS             3586..>3820
                     /locus_tag="VF357_06070"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7523747.1"
                     /translation="MKRVLVTGGAGFIGSHLCERLLAQGDEVLCVDNFFTSRRQNIAH
                     LRGNSLFEFMRHDITWPLYVEVDRIYNLACPASP"
BASE COUNT          665 a         1382 c         1190 g          583 t
ORIGIN      
        1 cccgtgaccg gcggcacggc gcgccacggc agcaagagcg tcctctatcc ggggaacggc
       61 gacacgatcg acttctgagc actccccggc ggagccccgc cctccgaggc ggcgcagccg
      121 aagggggagt cccggaggcg cagcactacc cgttccaccg ctcgatgagc gcgtagacga
      181 tccgtgtcat ctcttcgatc ccgtccaccg gaatccgctc gtccactccg tggatcttcc
      241 cgtgctcggc gcgcggcagc agcagcggca tcagcccgta ggtggggatg ccgatcgacc
      301 ggaagaagcg cgagtcggtg aagccggtgg acaggtacgg cacgacgacc gcgtcggggt
      361 gcacggcgag gaccgcctcc tccatcgcgc cgtagagcgg ccccatcggc gacccgttcg
      421 gcttctccgc gaagaggatc tcgaccgaca cgtcgaggtc cgcgaccagc gccctgaccc
      481 gcgccgccac cgtctccggc tcctcgcccg gcacgatccg cgcgtcgatc gtcccctccg
      541 ccgaagacgg gatcacgttc ggtttgaatc ccgccgcgag cgtcgtcacc gcgaacgtgt
      601 tccgcagcag agggtcgatc tcggggaacc ggcccccgag ggcttccagc gccccggcct
      661 cctcggctcc cccccgcagc gcatccacgt cgaggtccga gaatcctttc gcgcgcaaca
      721 cccgcagcat gtcccggacc ggctccgaaa gccgcgccgg ctcccggaac tcggccaccc
      781 gcgccatcgc ccgggccagc cgcgccgggg cgtccttcgc cgatggacgg ctgccgtgcc
      841 ccgcccttcc cgccgtggag agcttcatcc acaccggccc cttctcccac atgttcagca
      901 ggaagaactt cccgcgtccg ccgaagatgc cggtcacccc cacgccgccc tcgttcatcc
      961 cgtacgccgc gccgatcggg aacggcaggt tccgcacgaa atattccgct ccctcgcccc
     1021 cgccgacctc ctcgtccgcg ttcgccacga ggaacagttt tcttcgaagg atcccggcct
     1081 tcgcggcgcg cagcgcggcg caccagtggg cgacccccag gcccttcgtg tcgagcgtcc
     1141 cgcgaccgta caggaagccg tcccggatct gcgccgaaaa cggcggcacg gtccactcct
     1201 ccgccttcgc cggcacaacg tccatgtggt gaatgaggac gagccccggc tcctccgttc
     1261 cgccgacgtg acacagcaca ttcggtttct ccggcttcgc cccgaacgac accggcgaca
     1321 atccggtctg ccggagcgtc ccgcacagaa gttccgccgc tccccggcaa tcccccggag
     1381 gattcgatgt gtcgatcctc agatattcgg agagcagcgc gaccgggtcc gtcattgaag
     1441 ttccccccgt ttcaggatac attttcctga ccgcgcaaga gcggcccact tcgcgcgcca
     1501 ggggaaacac gcatgcccaa ggcactgatc accggcgtca ccggacagga cgggtcgtac
     1561 ctggccgagt tcctgctctc gatggggtac gaggtccacg gcatcatccg ccgcgcctcc
     1621 accttcaaca cgggccgcct ggaccacatc tacgtcgacc cgcacgccac cggcgcccgc
     1681 atgttcctgc actacggcga cctgtcggac ggcgggcagc tcaccaacct catctacaac
     1741 gtccagcccg acgaaatcta ccatttgggt gcgcaaagcc acgtgcgcgt ttccttcgac
     1801 atccccgaat acaccggcga catcaccggt ctcggcacga cccgcatcct cgagtcgctg
     1861 cgccgcgcgg gcggcaaggc ccgctactac caggcctcct ccagcgagat gttcggcgcc
     1921 accccgccgc cgcacggcga gaacacgccc ttctatcccc gctccccgta tggcgccgcc
     1981 aaggtgtact cctactggat ggcgatcaac taccgggaag cgtacaacat gttcgccagc
     2041 aacgggatcc tcttcaacca cgaatccccg cggcgcggcg agacgttcgt cacccgcaag
     2101 atcacccgcg cgatcgcgaa gatcaaggcg gggaagcaga aagacctgtt cctcggcaac
     2161 ctcgacgcac ggcgcgactg gggattcgcc tacgaatacg tccaggtgat gtggcgcatc
     2221 ctgcagcacg accgggggga tgatttcgtc atcggcaccg gccagtcgaa ttcggtgaag
     2281 gagttcctcg aggaggcgtt cgcctacgcg ggcctcgact ggaaggagta cgtgaagatc
     2341 gacccgaaat atttccggcc gacggaagtg gtgaacctga tcgcagacgc gtcaaaggcg
     2401 aagaagcggc tcggatggga gccgaaggtg acgttcaagg agctcgtgcg gatcatggtc
     2461 gacgccgacc tggaggacgc gggcctcccg acccccggcg agggccgcaa aatcctcgcc
     2521 gccaggggcc ttcccgtcgg ctcgaaactc taaggaatga gcacgtttac gggaaagaag
     2581 atcctggtca ccggcgggtc ggggttcctc ggggctcccg tcatcgcgca gctgctcggt
     2641 cgcggcgcgc ggcgggagga catcaccgtc ccccggtcgg cgcggtatga ccttcgcctc
     2701 ccgggtgcgt gcgcggaggt cgtcccggga caggacctcg tcatccacct ggcggccaac
     2761 gcgggcggga tcggctggaa ccggtcgcac ccgggcgctc tcttctacga caacgccgcg
     2821 atggggatcc acctgatgga ggagtcccgc aaggccggcg tgaaaaaatt cgtccagatc
     2881 ggcaccgttt gcgcctatcc gttccagccg ccgcgcatcc cgttccgcga ggacgacctc
     2941 tgggagggat atccggagcc caccaacgcg ccgtacggca tcgccaagaa ggcgatgatg
     3001 gtgatggggc atgcgtaccg ggaggagtac ggcttcaacg tgatctacct gctccccgtg
     3061 aacctgtacg gcccccgcga ccatttcttc gagccggaga agtcccacgt catccccgcc
     3121 ttgatacaga agttcgtcga cgcccgcgaa tccggcgcgc ccgaggtcgc ggtgtgggga
     3181 agcggcttct accggggcac ccccgtctcc cgcgaattcc tttacgtgga cgacgccgcg
     3241 gaagcgatcg cattggccgc cgagcggtac gacggggcgg aaccggtgaa cgtcggcgcg
     3301 ggccgcgaag tgccggtcaa cgacctggtc gccatggtgg gcgagatgac cggctaccgg
     3361 gggaaggtgg tccgcgacct cacgaagccc gacggccagc cccgccgctg tctcgacacc
     3421 tcccgcgcga aggagcggtt cggcttcgtc gcccgcacgt cgttcgagga aggtctgcgc
     3481 aagacgatcg cgtggtacga gcaggcgcga cgggaacggt gattcaccga cgggaacgtc
     3541 gccccgaacg gaacggatga aaattcggcg acacgagggg gagggatgaa acgcgttctg
     3601 gtcaccgggg gcgccgggtt catcggatcg cacctgtgcg agcggctgct ggctcaggga
     3661 gacgaggttc tgtgcgtcga caacttcttc acctcccgtc ggcagaacat cgcccacctc
     3721 cgcggaaact ccctgttcga gttcatgcgc cacgacatca cctggccgct ctatgtcgag
     3781 gtggaccgga tctacaacct ggcgtgcccc gcctcccccg
//
LOCUS       DASVJL010000287         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_702239, whole genome shotgun sequence.
ACCESSION   DASVJL010000287 DASVJL010000000
VERSION     DASVJL010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702239"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="VF357_06075"
     CDS             complement(<1..1505)
                     /locus_tag="VF357_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7523748.1"
                     /translation="MGPLRRPLSIRAGVVLQLALVATASLTLLAVFALKVIEVTMQRR
                     HVEAGISVAGIVRRAVEKEIAENPGSANISAGMFPGSLSPYINEVVLLPEPPPEGRPK
                     VVPVGEKAFPFLPVYPTVDVILPFGPLSPPAASAAASVPRGIRVRFHSPGIAAEVGTF
                     VNVTLLLAAIDIAVIVLFGGIFLDRTVISPVRKLASAAEKVAAGDYSLRVEGVEGNEV
                     GQLAASFNRMVEGILEAQERLRRSEKETFRSEKLATVGRLAAGVAHEVGNPLMAIRGY
                     AEHLLKHQPGAAESKECLDKVVEETKKIENIVRGLLSVASPGGGREGATDVNAVVRET
                     VEMLSYRNLFRDVEVRLELGETPRAAILADRFRQVLLNLVINAVDAMKGRGRLTVRTW
                     VMEGWSLGRRSPLRRRATDPPEMDSTPLRAAGAGTGEGVALAVTDTGGGIPAADLPLL
                     FDPFFTTKEPGKGTGLGLSVSRTIVDGAGGEIRAESEGGKGATFLVVLPSAR"
     gene            1576..2055
                     /locus_tag="VF357_06080"
     CDS             1576..2055
                     /locus_tag="VF357_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523749.1"
                     /translation="MGAVDDRRRTRIVVRQFQLGLAVRLLLVLTILFAAGLTLAFAPS
                     YYVLATTNDLKSLEPAAAEFLVLNKRLLPAALLSLAGIFIYAIFFSHRIAGPLYRINA
                     ILRALIEDRDPPVVNFRDDDYFQPTAALLKELSEKMRTSRRTTAPRPGGNPPSIDAK"
BASE COUNT          348 a          756 c          678 g          343 t
ORIGIN      
        1 cgggcggacg gcagcaccac gaggaaggtc gcccccttcc ccccctcgct ctccgcacgg
       61 atctcccccc ccgccccgtc gacgatcgtc cgcgaaacgg agaggccaag ccccgttccc
      121 ttgcccggct ccttggtcgt gaagaacggg tcgaagagca gcgggagatc ggcggccggg
      181 atccctcctc cggtgtcggt cacggcaagc gcgacacctt ctcccgtccc ggctccggcc
      241 gcgcgcagcg gcgtgctgtc catctccggc gggtccgtcg cacgccggcg tagcggggat
      301 cgtcgcccga ggctccaccc ctccatcacc cacgtccgca cggtaagtct tccgcggccc
      361 ttcatcgcgt ccaccgcgtt gatgacgagg ttcagcagca cctggcggaa ccggtccgcg
      421 aggatcgccg cccggggcgt ttcgccgagc tccaaccgaa cctccacgtc gcgaaacagg
      481 ttccggtacg agagcatctc gaccgtctcg cgcaccacgg cgttcacgtc cgtcgcccct
      541 tccctcccgc cgccgggaga cgccacggag aggagccccc ggacgatgtt ctcgatcttc
      601 ttcgtctcct cgacgacctt atcgaggcac tccttcgact ccgcggcgcc cggctgatgc
      661 ttcagcaggt gctccgcgta cccgcggatc gccatcagcg gattccccac ctcgtgggcg
      721 accccggccg cgagccgccc cacggtcgcc agcttctcgg atcggaacgt ctctttctcg
      781 gagcggcgca ggcgttcctg cgcctcgagg atcccctcga ccatccggtt gaaggaggcg
      841 gcgagctgcc ccacctcgtt cccctcgacg ccttcgacgc gcagcgagta atcccccgcc
      901 gcgaccttct cggcggcgga cgcaagcttg cggacggggg agatcacggt ccggtcgaga
      961 aagatccccc cgaagaggac gatgacggcg atgtcgatcg cggccaggag cagcgtaacg
     1021 ttcacaaagg tcccgacctc ggcggcgatc ccgggggaat ggaagcggac ccggatgccg
     1081 cgggggacgg acgcggcggc ggatgccgcg ggcggggaga gcgggccgaa cgggaggatc
     1141 acgtccaccg tgggatacac cgggaggaac gggaacgcct tctcccccac ggggacgacc
     1201 tttgggcggc cctcgggcgg cggttccggc agcaggacga cctcgttgat gtagggagaa
     1261 agggaaccgg ggaacatccc ggcggagatg ttcgcggacc ccgggttctc cgcgatctcc
     1321 ttttccaccg cccttcggac gatcccggcg acggagatgc cggcctcgac gtgacggcgc
     1381 tgcatggtga cctcgatgac cttcagcgcg aagacggcga ggagcgtaag ggatgcggtg
     1441 gccaccagcg caagctgcag cacgacgccg gcgcggatcg aaagcgggcg ccttagcggg
     1501 cccatagaaa ggggatcggc gttgactctg ctctcacgga gattagaata gcaaaaacgg
     1561 atgggggagg cgaatttggg cgcggtcgat gatcgtcgtc ggacgaggat cgtggtccgg
     1621 caattccagc tggggcttgc cgtgcggctc ctcctggtgc tcacgatcct cttcgcggcg
     1681 gggctcaccc ttgcgttcgc tccgtcgtac tacgtcctcg cgacgacgaa cgacctgaaa
     1741 tccctcgagc ctgccgctgc cgagttcctc gtgcttaaca agcgcctctt gcccgcagcg
     1801 ctcctctccc ttgcagggat cttcatctat gcgatcttct tcagccaccg gatcgccggg
     1861 ccgttgtacc ggatcaacgc gatcctccgg gcgctcatcg aagaccggga tccccctgtc
     1921 gtgaattttc gcgacgacga ctacttccag cccaccgccg cgctgctgaa ggagttgtcg
     1981 gagaagatgc gcacctcgcg gagaactacg gcaccccgcc ccggtggcaa tcccccatcc
     2041 atcgatgcga aatgaaccgg ttcacccctg cggatcatcg caacggcagg agcgggttca
     2101 cgctgatcga gatcgccctc gtcct
//
LOCUS       DASVJL010000288         6130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_984242, whole genome shotgun sequence.
ACCESSION   DASVJL010000288 DASVJL010000000
VERSION     DASVJL010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984242"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..633
                     /gene="raiA"
                     /locus_tag="VF357_06085"
     CDS             94..633
                     /gene="raiA"
                     /locus_tag="VF357_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX7523750.1"
                     /translation="MNQINVTFRHVDPSQALKDYVTGKLGKIGKVVEKSFDAHVTLSV
                     EKYRHIAEVFLTARGITIKAFESTEDLYSAIDLVCDKVERQLKKYREKKKDKGQAILP
                     EPMVSGSSLTIAEGEGRPRIIHSDNFLAKPMTVEDAARHLDMLRLDVVMFVNQETNQP
                     SVVFRQQDGNIGFTEPPAR"
     gene            630..1091
                     /locus_tag="VF357_06090"
     CDS             630..1091
                     /locus_tag="VF357_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX7523751.1"
                     /translation="MRIQDIIPPEAVVDGLRAETKEGVLRELSEAVCRRLPALSPDRL
                     TAILMDREGLGSTGIGEGVAIPHGKIPGIDRLVSVFGRSRGGVQFASLDGKPARLFFL
                     ILAPENSAGMHLKALARISRLLKDQRFRGRLLAAEGAEGLSQVLREEDERA"
     gene            1108..2046
                     /gene="hprK"
                     /locus_tag="VF357_06095"
     CDS             1108..2046
                     /gene="hprK"
                     /locus_tag="VF357_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX7523752.1"
                     /translation="MPISVQTFRDECSAPLRLRLLAGERGMGRGIAGSRVQEAGLAIA
                     GEVFPSREGFVQVLGETEVDYFHRQDPARQPEIAERFFSGPLPCAVVVGPLPAPGLFV
                     EAAERASVALFASDFPAAGFLEEAGRQLYRLFTETTTVHGVLLDVIGVGVILFGRSGI
                     GKSECALDLVLRGHRVVADDVIHVDKLGPATLVGRGDDLTCHHMEIRGLGIINVRDLF
                     GATATTRMKKIELVIRIEEWDPSREYDRLGIEDGTVEILGVRLPFLLVPVSPGRNLAT
                     IVEVAVRNHLLKQLGVHSAREFIERQARRAGGSGTP"
     gene            2043..2951
                     /gene="rapZ"
                     /locus_tag="VF357_06100"
     CDS             2043..2951
                     /gene="rapZ"
                     /locus_tag="VF357_06100"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX7523753.1"
                     /translation="MIIGDARPHRADIVVVTGLSGAGKTTAIKVFEDLGYYCVDNLPP
                     VLLPRIVDVVSEARGEGARVALGMDIRGKEFLPDLSRVLDDLRRGRNAVHVLFLDAAD
                     EALVRRFSETRRKHPLAARGGALGAIRKERRILSPLREMADAVIDTSEFNVHQLRDAV
                     VRRFRREGAGDLKVSVISFGYRYGIPVEADMVVDVRFLANPNFVPALKRFTGLDRGVQ
                     DYVLGARATKGFLRRLSDLLLFLLPLYRKEGKAYFTLGVGCTGGRHRSVAVAEALGDI
                     LGKGKEAAVVIHRDLSRSSLPAKGMR"
     gene            2948..3370
                     /locus_tag="VF357_06105"
     CDS             2948..3370
                     /locus_tag="VF357_06105"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX7523754.1"
                     /translation="MIGAVVISHGALATELVRAAGIIVGKIEGIVAVDISPDMSVEAV
                     HDAVDGAVRTVEDGDGVLLLTDMFGGTPSNIGLSFLGTHRVEVLTGVNLPMMVKFPMA
                     REGMPLDELARQLQECGQRSIMIPGDMLKKKAEKKPEK"
     gene            3375..3851
                     /locus_tag="VF357_06110"
     CDS             3375..3851
                     /locus_tag="VF357_06110"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEX7523755.1"
                     /translation="MPLVLARIDCRLIHGQVVETWVPHKSADSLIVANDDLAGNPFLR
                     SVMELAVPPAIRVRFCRLDEAIPALADADRNRERSILLVASTADAVTLRKAGAAFDLL
                     NIGNLHFAAGKVGISPSVFFAPEDFDALGWFRSHGVSVLIQGTPFDAGTSFDAERE"
     gene            3854..4552
                     /locus_tag="VF357_06115"
     CDS             3854..4552
                     /locus_tag="VF357_06115"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEX7523756.1"
                     /translation="MAWRFLLAILLGGVCYLDRTAACQLMLHRPLVVATLMGAVFGNM
                     AAGAQVGVVLELIYLARLPVGASIPPDDTGAAVFGGAAAAIASSSIGLDAGSFTALLL
                     LSVFVAEAGKAADRFVRKVNVRIARITAESVDRGDVQAVEHGLLAGLTLFAVTGMVLA
                     LVFSGLGVAVSKELLPRFGPASRSNFAILLPALPLLGAASVYSCSRAERTAAVFFLTM
                     AAVFGATVLFRWSA"
     gene            4549..5313
                     /locus_tag="VF357_06120"
     CDS             4549..5313
                     /locus_tag="VF357_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEX7523757.1"
                     /translation="MTTPALARRSRSAVWRRQFLLQGCWNYEGMQNVGFAYAILPALR
                     DFYASRPEDALKAVKRHLEFFNTQPAMGALILGASVRLEERVAAGEADPRAIGTFKVG
                     LMGSLGAIGDSFFWGALRPMASVAGALLALLHPLLGIAAMLLLYNGVHLMVRRRGFAA
                     GMEGPEAVVGWLKRAAFNVRTDDRKLLAAILAGAYAGVFAGHFFVQGGGAPHAVASLL
                     LAGAAVHLFSILFRKAVSPSEILSFLLFVGVLILWR"
     gene            5310..5579
                     /locus_tag="VF357_06125"
     CDS             5310..5579
                     /locus_tag="VF357_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX7523758.1"
                     /translation="MSVEREFDILNRLGLHARAAAQLVRLANGFASEIHVVKDGLEIN
                     GKSIMGVLMLAAPKDTKILIRAIGLDADEAMAAIGELIAQKFGEE"
     gene            5585..>6130
                     /locus_tag="VF357_06130"
     CDS             5585..>6130
                     /locus_tag="VF357_06130"
                     /inference="COORDINATES: protein motif:HMM:NF017346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HEX7523759.1"
                     /translation="MTEGREKMRVLKGIPASGGVAIGKGFFLNRVLPRSVRSTVGRDQ
                     VDEEVGAFQRAVARSREQILQIRDGVADPTSEHHQILSVHLALLEDSMLVEQTVRTIR
                     ENQFAADWAFNKVLQNLLETFHRIEDPYLRERGHDLRQIGHRVLENLAGRPVDSVAGI
                     RDPVIIVAHDLSPADTAQILKS"
BASE COUNT          947 a         1956 c         2170 g         1057 t
ORIGIN      
        1 aagtaccgcg cggcgatggg actgctggcc tcgtcgcggc gcaggaagca gtactgagcg
       61 ccaaagcacc gattccgctg aaggagggca cccgtgaacc agatcaacgt gacgttccgg
      121 catgtcgacc cgagccaggc attgaaggat tacgtgaccg ggaaattggg aaagatcggg
      181 aaggtggtcg aaaagtcgtt cgacgcgcac gtcaccttgt ccgtcgagaa ataccgccac
      241 atcgccgagg tgttcctgac ggcgcggggg atcaccatca aggcgttcga atccaccgaa
      301 gacctgtact ccgccatcga cctcgtgtgc gacaaggtcg agcgccagtt gaagaagtac
      361 cgcgagaaga agaaggacaa ggggcaagcg atcctccccg agccgatggt gtccggatcg
      421 tccctcacga tcgcggaggg ggaaggccgg cccaggatca tccactcgga caacttcctg
      481 gccaagccga tgacggtgga ggatgcggcg cgccacctcg acatgctccg gctcgacgtg
      541 gtgatgttcg tgaaccagga gacgaaccag ccgagcgtgg tcttccggca gcaggacggc
      601 aacatcggct tcacggagcc gccggctcga tgaggatcca ggacatcatt cctccggagg
      661 ccgtggtgga cgggctccgg gcggagacga aggagggggt ccttcgcgag ctctcggagg
      721 cggtgtgccg gcggctcccg gccctttccc cggaccgcct cacagcgatc ctgatggacc
      781 gcgaggggct cgggagcacg ggaatcggcg agggggtggc gattccccac gggaagattc
      841 ccgggatcga ccggctggtc tccgtcttcg ggcgcagccg cgggggggtg cagttcgcct
      901 ccctcgacgg gaagccggcc cggctctttt tcctgatcct cgccccggag aattcggcgg
      961 ggatgcacct caaggcgctc gcgcgcatct cgcggctcct caaggatcag cggttccggg
     1021 ggcggctcct ggcggccgag ggggccgagg ggctttcgca ggttctccgg gaagaggacg
     1081 agcgcgcctg agctgaaagg gggggccgtg ccgatctccg tgcaaacctt ccgggacgag
     1141 tgctcggcgc ccctgcgcct ccgtctcctc gcgggggagc gcgggatggg gcgagggatc
     1201 gcgggaagcc gggttcagga agccgggctg gcgatcgccg gcgaagtctt cccgtcccgg
     1261 gaagggtttg tccaggtcct gggcgagacc gaggtggact acttccacag gcaggatccg
     1321 gcgaggcagc cggagatcgc cgaacggttc ttctccggcc ccctgccgtg cgcggtagtc
     1381 gtcggaccgc tcccggcgcc gggtctcttc gtcgaagcgg cggagcgggc ttccgtcgcc
     1441 ctgttcgcgt ccgacttccc cgcggccggt tttctcgaag aggcgggccg ccagctctac
     1501 cgtcttttca cggaaacgac cacggtccac ggggtcctcc tcgatgtcat cggtgtgggg
     1561 gttatcctct ttggacgaag cgggatcggc aagagtgaat gcgccctcga cctcgtcctt
     1621 cggggccacc gggtcgtggc cgacgacgtt atccacgtcg acaagctcgg cccggccact
     1681 ctcgtgggcc gcggggacga tctcacctgc caccacatgg agatccgggg gctcgggatc
     1741 atcaacgtcc gggacctgtt cggcgcgacc gcgaccacgc ggatgaaaaa gatcgagctc
     1801 gtgatccgga tcgaggaatg ggatccttcc agggagtacg accggcttgg tatcgaggac
     1861 gggaccgtcg agatccttgg cgtccgactt cccttccttc tcgtcccggt ctcccccggg
     1921 cggaacctgg cgacgatcgt ggaggtggcg gttcggaacc acctgctgaa gcagctcggg
     1981 gtccactccg cgcgtgaatt catcgagcgc caggcgcgtc gcgccggagg gagcggcacc
     2041 ccatgatcat cggcgacgcg cggccgcacc gtgccgacat cgtcgtcgtg accggactgt
     2101 cgggggccgg gaagaccacc gcgatcaagg tgttcgagga tctcggctac tattgcgtgg
     2161 acaacctgcc gccggttctc cttcccagga tcgtcgacgt cgtctccgag gcgaggggag
     2221 aaggggcccg ggtggccctc gggatggaca tccggggaaa ggagtttctc ccggacctct
     2281 ccagggtcct cgacgatctg cggagggggc ggaacgcggt ccacgtcctc ttcctcgacg
     2341 cagcggacga ggcgctcgtg cggaggttca gcgaaacgcg ccggaagcac cctctcgcgg
     2401 ccaggggcgg cgccctgggc gcgatccgga aggagcggag gatcctctcg ccgctgcggg
     2461 agatggcaga cgccgtcatc gatacctccg agttcaacgt ccaccagctt cgagacgccg
     2521 ttgtgcgacg gttccgccgc gagggggccg gcgacctgaa ggtgagcgtc atctccttcg
     2581 gttaccggta cgggataccc gtcgaagccg acatggtggt cgacgtccgg ttcctcgcca
     2641 acccgaactt cgttccggca ctcaagcgat ttaccggtct tgaccgggga gtccaggact
     2701 atgtcctggg ggcccgcgcg acaaaggggt tccttcgtcg tctgtcggat ctgttgcttt
     2761 tcctccttcc cctctataga aaggaaggga aggcgtactt cacgctggga gtcggctgca
     2821 ccggcggcag gcatcggtcc gtcgcggtcg cagaagcgct cggcgatatt ctcgggaagg
     2881 ggaaggaagc ggccgtcgtg atccaccgcg acctgtcgcg ctcctccctg cccgccaagg
     2941 ggatgagatg atcggagcgg ttgtcatctc ccacggcgcc ctcgctacgg aactggtgcg
     3001 cgccgccggg atcatcgtcg gaaagatcga ggggatcgtc gccgtcgaca tctcccccga
     3061 catgagcgtc gaggcggtcc acgacgcggt ggatggggcc gtccgcacgg tcgaggacgg
     3121 tgacggcgtg ctgctcctca ccgacatgtt cggcggaacg ccctccaaca tcggcctgtc
     3181 gttcctcggc acgcaccgcg tggaagtcct gacgggggtg aacctgccga tgatggtcaa
     3241 attccccatg gcccgggaag ggatgccgct agacgaactc gcccgccagc tgcaggagtg
     3301 cggacagcgc agcatcatga tcccgggaga catgctgaag aagaaggccg aaaagaagcc
     3361 ggagaagtag cgggatgccc ctggtgctcg cccgcatcga ctgccgcctg atccacgggc
     3421 aggtggtgga gacgtgggtg ccccacaagt ccgccgattc cctgatcgtc gccaacgacg
     3481 acctggccgg gaacccgttc ctgcgctctg tgatggagct ggcggttccc ccggcgatcc
     3541 gcgtccgttt ctgtcgcctc gacgaggcga ttcccgccct cgcggacgcc gaccggaaca
     3601 gggagcgctc catcctgctg gtcgccagca cggcggacgc ggtgacgcta cggaaggcag
     3661 gggcggcgtt cgacctcctg aatatcggaa atctccactt cgccgcgggg aaggtcggga
     3721 tctccccgtc cgtattcttc gccccggagg atttcgacgc cctcgggtgg ttccgctcgc
     3781 acggggtatc ggtcctcatc caggggacgc cgttcgatgc gggaacgtcg ttcgacgcgg
     3841 aaagggagta agggtggcct ggcggttcct cctggcgatc cttctcgggg gggtgtgcta
     3901 cctcgaccgg acggcggcgt gtcagctgat gctccaccgc ccccttgtcg tcgccacgct
     3961 gatgggagcg gtcttcggga acatggccgc cggcgcccag gtgggggtcg tcctcgagtt
     4021 gatctacctg gcccggctcc ccgtgggggc gtcgatccct cccgacgaca cgggcgcagc
     4081 cgtgttcggc ggcgcggccg ccgcgatcgc ctcttcctcc atcgggcttg acgcggggag
     4141 tttcaccgcc cttctgctgc tttccgtctt cgtcgcggaa gcgggaaaag cggcggaccg
     4201 tttcgtccgg aaggtcaacg tacggatcgc ccgcatcacg gcggagtcgg tggaccgggg
     4261 ggatgtgcag gcggtggagc acgggctgct cgccggcctc accctgttcg cggtgacggg
     4321 gatggtcctt gcgctcgtct tctcgggatt gggcgtggcg gtgtcgaagg agttgcttcc
     4381 ccggttcggc cccgccagcc ggagcaactt cgcgatcctc ctgccggcgc ttccgctcct
     4441 gggtgccgcc tcggtctatt cgtgcagccg cgcggagcgg accgccgccg tgttcttcct
     4501 gacaatggcg gccgttttcg gcgccaccgt gctgttccgg tggtcggcat gacgacgccg
     4561 gcgctcgcga ggcggtcccg tagcgccgtc tggcggcgcc agttcctgtt gcaggggtgc
     4621 tggaactacg aggggatgca gaacgtggga ttcgcctacg ccatcctgcc ggcgctgcgc
     4681 gacttctacg cgagccgccc ggaggacgcg ctgaaggcgg tgaagcggca cctcgagttt
     4741 ttcaacaccc agccggcgat gggggcgttg atcctggggg cgtcggtccg actcgaggaa
     4801 cgggtggccg cgggtgaggc ggatccgcgg gcaatcggta cgttcaaggt ggggctgatg
     4861 gggtcgctgg gggcgatcgg ggactccttc ttctggggcg ccctgcggcc gatggcctcg
     4921 gtggcggggg ccctccttgc gctgctccat cccctcctgg ggatcgccgc catgctgctg
     4981 ctctacaacg gagtccacct gatggttcgc cggcgcggtt tcgccgccgg catggaggga
     5041 ccggaggccg tggtgggctg gctcaagcgg gcggcgttca acgttcggac cgatgatcgg
     5101 aagctgctgg cggcgatcct cgccggggcg tacgccgggg tcttcgccgg gcattttttc
     5161 gtccagggag ggggcgctcc ccacgcggtg gcgtcgcttc ttctcgccgg cgccgccgtg
     5221 cacctgttct ccatcctgtt ccggaaggcg gtctccccgt cggagatcct gtcgtttctt
     5281 ctcttcgtgg gggtgctgat cctgtggcgg tgagcgtgga acgcgagttc gacatcctga
     5341 accggcttgg cctgcacgcc cgcgccgccg cgcaacttgt gcgccttgcg aacgggttcg
     5401 cctcggagat ccacgttgtg aaggacgggc tggagatcaa cggaaagagc atcatgggcg
     5461 tgctgatgct ggcggcgccg aaggacacga agatcctgat ccgcgcgatc gggctggatg
     5521 cggatgaggc aatggcggcg atcggggaac tgatcgcaca gaaattcggg gaggagtagg
     5581 aggggtgacg gaaggccgcg agaagatgcg ggtcctcaag gggatcccgg cctcgggagg
     5641 ggtcgcgatc gggaaggggt tcttcctgaa tcgcgtcctc ccgcggtcgg tccgctcgac
     5701 ggtcggtcgc gatcaggtgg acgaggaggt cggcgccttc cagcgggcgg tggcccgctc
     5761 ccgggagcag atcctccaaa tccgggacgg cgtggcggac cccacctccg agcaccacca
     5821 gatcctctcg gtccacctgg ctctcctcga ggactcgatg ctcgtcgagc agacggtccg
     5881 gacgatccgc gaaaaccagt tcgccgcgga ctgggcgttc aacaaggtgc ttcagaacct
     5941 cctggagacg ttccaccgga tcgaggatcc gtacctgcgg gagcggggac acgacctgcg
     6001 ccagatcggc caccgggtcc tcgaaaacct cgccggccgc cccgtggact cggtcgccgg
     6061 gatccgtgac ccggtgatca tcgtggccca cgatctctcc ccggcggaca ccgcgcagat
     6121 cctgaagagc
//
LOCUS       DASVJL010000289         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_984382, whole genome shotgun sequence.
ACCESSION   DASVJL010000289 DASVJL010000000
VERSION     DASVJL010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984382"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VF357_06135"
     CDS             <1..593
                     /locus_tag="VF357_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEX7523760.1"
                     /translation="HVDDAVRMAEELGAASVKFNLVQPTARGESLHRERETLSVAELI
                     ELGRHVARELTQRISLRLFFDYPQAFRSLSRIAGGEGCTVCGIFGILGVTADGHYALC
                     GIGEQVPDLVFGVVGKDPLEEVWRDHPVLKSMRVGLPGRLEGVCSRCLMKQRCLGACV
                     AQNYYSEGRLWAPYWFCQQAEEEGLFPASRLGKRSA"
     gene            623..1276
                     /locus_tag="VF357_06140"
     CDS             623..1276
                     /locus_tag="VF357_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523761.1"
                     /translation="MPRTRYKQKKDLGEAAEAAFFAAYVGPSMNPTLREPEIMEIVPC
                     GGRSLRAGDVVLFLLPGSDRPVVHRVARATTAGISTRGDNNAMEDHVLLQFEDIQGRV
                     VAAWRGRKRREIAGGFRGRWNGRWLRVQRLMDRGVSPLLHPLYRSLSLRGRFARCLPA
                     SFRPRVVVFRDQGQDRFRLLLGERVIGRYEEQERQWRIQRPFRLLVDERTLPGRTEP"
     gene            1507..>1722
                     /locus_tag="VF357_06145"
     CDS             1507..>1722
                     /locus_tag="VF357_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523762.1"
                     /translation="MLPSEADGTVRCVLPSSGLDDQLYIQLMLLSGVIARDAQVRGGI
                     LLHGALAERDGVGAVLAASGGTGKTTAS"
BASE COUNT          271 a          516 c          610 g          325 t
ORIGIN      
        1 gccatgtgga cgacgcggtg agaatggcgg aagaactcgg tgcggcctcg gtcaaattca
       61 acttggtgca gcccaccgcg cggggcgaat cgctgcatcg ggagcgggaa accctgagcg
      121 tcgccgaact gatcgaactg gggcggcacg tggcacggga actgacgcaa aggatcagcc
      181 tgcgactctt tttcgactac ccgcaggcgt tccggtcctt gagccggatc gccgggggag
      241 aaggttgcac tgtctgcggg atcttcggga tcctcggcgt caccgccgac gggcactatg
      301 ccctgtgcgg gatcggcgag caggtgccgg acctggtctt cggcgttgtg gggaaggatc
      361 cgctggaaga ggtgtggcgg gaccatcccg tcctgaagtc gatgcgggtc ggattgcccg
      421 ggcgactgga aggggtctgt tcccgctgcc tgatgaaaca gcgctgcctg ggcgcgtgcg
      481 tcgcccagaa ctattacagc gaaggcaggc tgtgggcgcc gtactggttc tgccagcagg
      541 cggaggagga aggcctcttt cccgcatcgc gccttgggaa acgatccgcc tgacctattc
      601 ccgttggcaa cccaaggaga gcatgccccg gacaagatat aagcagaaga aggatcttgg
      661 agaggccgcc gaggcggcgt tcttcgccgc ctacgtcggc cccagcatga accccacgtt
      721 gcgcgagccg gagatcatgg agatcgtgcc ttgcggcggc agatccttgc gggccgggga
      781 cgtagtgttg ttcctcctgc cggggtcgga tcgcccggtc gtgcaccgcg tcgcccgtgc
      841 gaccacggcg gggatctcca cgcgcggcga caacaatgcc atggaggatc acgtactcct
      901 gcagtttgaa gacatccagg gccgggtggt ggccgcctgg cgtggccgga agcggcggga
      961 gatcgcggga gggttccggg gccgttggaa cggtcgctgg cttcgtgtgc agcgtctcat
     1021 ggaccgcggg gtttctcccc tgctccaccc cctgtatcgc tccctgtccc tgagaggaag
     1081 gttcgcccgg tgtctgccgg cttccttccg gccgcgggtc gtcgtcttcc gggatcaggg
     1141 gcaggatcga ttccggctcc tgctggggga acgggtcatc gggcgatacg aggagcagga
     1201 gcgccaatgg cggattcaac ggccgtttcg cctgttggtg gatgagcgga cgctcccggg
     1261 acgtaccgaa ccgtgagctt tcctcttccg gtggaatggg aatatcctct ttccctggcg
     1321 gatggaagtt gctggttctt cgccgccgcg aacgcggatg ccgagccgat cgtctcaaga
     1381 tttggggacg tgatgcggct gcgtggaagg agcccggcgg cttcgcctcc tcccggcatc
     1441 gtgcgccgtc ttctcgtatc ggtagacccc cgtccttcag aagcctcccc gttttcatgg
     1501 gaagcgatgc ttccatcgga ggcggacggg accgtacgtt gcgttcttcc ttcatccggc
     1561 ctcgacgatc aactgtacat tcagttgatg ctgttatcgg gagtgatcgc ccgggatgca
     1621 caggtccggg gagggatcct gttgcacggc gccctcgcgg agcgggacgg cgtcggggcg
     1681 gtcctggcgg cctcgggcgg cacgggaaag accaccgcca gc
//
LOCUS       DASVJL010000290         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_516018, whole genome shotgun sequence.
ACCESSION   DASVJL010000290 DASVJL010000000
VERSION     DASVJL010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516018"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VF357_06150"
     CDS             <1..677
                     /locus_tag="VF357_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7523763.1"
                     /translation="ELLRRIAAIKGISRIRFITSHPRDLDDRTIRLFEEIETLCPHVH
                     LPLQSGSDRVLEAMGRGYTRGEYLAKIEALRQVRPGMAFSSDFIVGFPGETVDDFRET
                     LAVMGEIRFDSSFSFRFSPRPGTRAAEMKEKVDPQEAAARLRRLQDLQTAHTRERLTA
                     LVGREITVLVEGTSTRNPEMRCGRTACNKTVNFTPGSAEGAIRSILVTGVGTHSLVGE
                     ERTSYA"
     gene            670..1158
                     /locus_tag="VF357_06155"
     CDS             670..1158
                     /locus_tag="VF357_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX7523764.1"
                     /translation="MPKEVTVAGVTIDPVTKSPIVILREPESGNVIPIWIGLLEANAI
                     ALALEGTELPRPMTHDLMKSILHATGTRLRSIEIVDIRESTYFALLHIEGNGGSFRVD
                     ARPSDAIALALRCGARILVSETVLAQSLVPATAMSESGERDKWTELLEKMDPELFSKY
                     KM"
     gene            1173..>2824
                     /locus_tag="VF357_06160"
     CDS             1173..>2824
                     /locus_tag="VF357_06160"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7523765.1"
                     /translation="MKTSRILIFEERPSPGRELRLNLLDHAVEFDFRDPAKEDLEALD
                     LSHYAAIVAPVESARAGLISVLSDAKRYGGPLFLYRDEPPVHEVARWVIWGSPSHGGP
                     DGPVADRLLAESLEYYSMASMYQQCLEMMSTQDEEKLLAQVTETFVNTLGAASCVIWL
                     VSPSDPDEMLIASVRGVIGIDREGSRFFLSRSDTAEEIWKGDPFPVAKGGERGARNGL
                     DIIVPLHHQGNPIGLVKLGERNDRKPFGDRELHMARIIADYAAGALNTVDRLGRIKKA
                     SLRDPETGAYSAAFLADYFEKERYKAGRFRRPLSVVFLVVENFSFLMEQTRESIVVGA
                     LTSMVNAVRRAVRDSDLVAREEANRFSIVLPETDAFGALLAVRRLRKAVMEKCRIQFL
                     GTEFFLQPFFMSATCPRDGREFPELLRVAEEKFARQKKSPLHRMRLAERPFWDAFEIL
                     VGKRDHYDLLRKGEDVPYFQRFRKDLGRNAHFSVPRETWLRILEAVAQDVAATPEESG
                     LVIAAGPTPEIYKQIFLSFKAFIPPRRNVYIVGQSGSTRFDS"
BASE COUNT          519 a          913 c          896 g          496 t
ORIGIN      
        1 cggagctgct gcggcggatt gccgcgatca aagggatatc gcggatccgg ttcatcacct
       61 cccacccgag ggacctggat gaccggacga tccggctctt cgaggagatc gagacccttt
      121 gcccgcacgt ccaccttccg ctgcaatccg gctcagaccg tgttctcgag gccatgggac
      181 ggggctacac gcgcggggag tatctcgcga agatcgaagc gttgcggcaa gtccgcccgg
      241 gtatggcgtt ctcatccgac ttcatcgtcg gcttcccggg ggagacggtg gacgattttc
      301 gggagaccct cgccgtcatg ggggagatcc ggttcgactc atcgttctcg ttccgcttct
      361 ctccccggcc cgggacccgg gcggcggaga tgaaggagaa ggtcgacccg caagaggcgg
      421 ccgcgcggct acggcgtctg caggaccttc aaacggcgca cacccgggaa cgccttacgg
      481 cgctggttgg tcgggaaatc acggttctcg tcgaaggaac aagcacgaga aacccggaga
      541 tgcgttgcgg tcgaaccgcc tgcaacaaga cggtgaactt tactcccggg agcgccgaag
      601 gggcgatccg gtctatcctc gtgaccggcg tggggaccca ctccctcgtt ggggaggaaa
      661 ggacgtccta tgcctaaaga ggtcacggtg gcaggcgtca cgatcgatcc cgtaacgaaa
      721 tctccgatcg tcatccttcg ggaaccggag tccggcaacg tgatcccgat ctggatcggc
      781 ctgctcgaag ccaacgcgat cgcgctggcg ctcgagggga cggagctgcc gcggccgatg
      841 acgcatgacc tcatgaagtc gatcctccat gccaccggaa cgcggcttcg aagcatcgag
      901 atcgtggaca tccgcgagag tacgtacttc gccctccttc acatcgaagg gaacggtggg
      961 agcttccgcg tggacgcgcg tcccagcgac gccatcgcac tcgccctgcg ctgcggagcg
     1021 cgcatcctgg tatccgaaac ggtgctcgct caatcccttg tccccgctac cgcgatgtcc
     1081 gaaagcggtg agagggacaa atggaccgag ctgctcgaga agatggaccc tgagctattc
     1141 agcaagtaca agatgtaaag cctccggcag cgatgaaaac atcccggata ctgatcttcg
     1201 aggaacggcc ctcccccggc agggagctgc ggcttaacct cctcgatcat gccgtcgagt
     1261 tcgacttccg cgatcccgcg aaggaggatc tcgaggcact cgacctctcc cactacgccg
     1321 ccatcgtcgc tccggtagag tccgcgcggg ccggcctgat cagcgtcctg tccgacgcga
     1381 agcggtacgg aggccccctg ttcctgtacc gcgatgaacc gccggtgcac gaggtggcgc
     1441 gatgggtcat ctggggttcc ccctcccacg gcgggccgga tggcccggtg gccgaccgcc
     1501 ttctcgcgga gagcctcgag tactactcga tggcatccat gtaccagcaa tgcctcgaga
     1561 tgatgtccac gcaggacgag gagaaactgc tggcccaagt cacggagacg ttcgtcaaca
     1621 ccctcggggc tgcgagctgc gtcatctggc tcgtttcgcc ctccgacccg gacgagatgc
     1681 tgatcgcttc cgtgcgcggc gtgatcggga tcgatcggga ggggtcccgc ttcttccttt
     1741 cgcgctcgga cacggcggag gagatctgga agggggatcc gtttcccgtc gcaaagggag
     1801 gggagagggg tgcccgcaac gggttggaca tcatcgttcc gctccaccac cagggaaacc
     1861 cgatcggcct cgtaaagctc ggggaacgga acgacaggaa gcctttcggc gaccgggagc
     1921 tccacatggc ccggatcatc gccgactacg ccgccggcgc cctgaatacg gtcgatcgcc
     1981 tcggacggat caagaaggct tccttgcgcg atccggagac cggcgcctac tccgcggcgt
     2041 tcctcgccga ctatttcgag aaggagcgat acaaggcggg ccgattccgc cggccgctct
     2101 ccgtcgtatt cctcgtcgtc gagaacttct ccttcctgat ggagcagacg cgggagagca
     2161 tcgtcgtggg agcgctgacg tccatggtca atgcggtccg ccgggcggtg cgggattcgg
     2221 atctcgtcgc gcgggaggag gcgaaccggt tttccatcgt ccttcccgag accgacgcct
     2281 tcggagcctt gctcgccgtt cgacggctcc ggaaggcggt aatggaaaag tgcaggatcc
     2341 agttcctcgg gacggagttt ttcctccagc cgttcttcat gtccgccacc tgcccgaggg
     2401 acggccggga attccccgaa ctgctgcgcg tcgccgagga aaaattcgcg cggcagaaga
     2461 agagcccgct gcatcgcatg cgcctcgccg aacggccctt ctgggatgcc ttcgaaatcc
     2521 ttgtcgggaa acgggaccac tacgacctcc tcaggaaagg ggaggatgtc ccgtacttcc
     2581 agcgcttccg gaaggacctc ggacggaacg cccacttctc cgtccctcgt gaaacctggc
     2641 tgcggattct cgaggcggtg gcccaggacg tcgccgcgac ccccgaggag agcggcctcg
     2701 tcatcgccgc ggggccgacc ccggagatct acaaacagat cttcctctcc ttcaaggcct
     2761 ttatcccacc gcgcaggaac gtttatatcg tcggccagtc ggggagcacc cggttcgact
     2821 cgaa
//
LOCUS       DASVJL010000291         3380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_516162, whole genome shotgun sequence.
ACCESSION   DASVJL010000291 DASVJL010000000
VERSION     DASVJL010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516162"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..777)
                     /locus_tag="VF357_06165"
     CDS             complement(169..777)
                     /locus_tag="VF357_06165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7523766.1"
                     /translation="MDDLKPFPVKPLLVFVAVIAAVRLSLSLPSTPFFSPSLLAAALF
                     LYAPLPRYWRRGFPAWARATDPGRSVAFFALLAGAGAIAFFLFVRLPLPPAISPYHGT
                     VPLTAAMAAHHLLLVALPEEVFFRGYLYDAFEEAGWEPVLPVALLFAIGHFVIAPSPF
                     RLLTFFPAVLLGWGRKRSGNVYVPTALHFLYNLFPSLIGGST"
     gene            complement(788..1444)
                     /locus_tag="VF357_06170"
     CDS             complement(788..1444)
                     /locus_tag="VF357_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7523767.1"
                     /translation="MEYAARLLIFDLDGTLVDSKVDLAIAVNVALESFDLPPLPHPTI
                     YSYVGDGASALILRALPQEKADLLPEVLDRFLAYYRRHLLDTTRVYPGVVGALRKWAG
                     IYRMAVLTNKGVAMTREILSGLSLDGYFFEVQGGDSFGTKKPDPEGLLHILRETRVEA
                     EEAIMIGDSRNDVQAGRAAGTITCGVTYGLGASGFAESPPDFTVDRFPDLFTRIRPAG
                     "
     gene            1603..2586
                     /gene="bioB"
                     /locus_tag="VF357_06175"
     CDS             1603..2586
                     /gene="bioB"
                     /locus_tag="VF357_06175"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX7523768.1"
                     /translation="MERFWERARTKAMSGEGIGREDALAVLSLGNDALWRLLDVTESV
                     RRRFKGDGIRLCSIVNAKSGLCSEDCAFCAQSRQSAAGIGKYPLMSGEEIFREAAAAK
                     ERGAREFSIVASGLAMRNREELTRVGDAVDRIRTELGLETCVSLGTLPPSDVEYLLSR
                     GLRSVHHNLETSRSFFPKVCTTHDYEEDVAAVRAAKAAGAWVCCGGIFGLGESPEDRV
                     ELALSLRELRVDSIPVNFLNPVAGTPLEGRRELSPMDCLRIIAMLRLTNPTREIIVCG
                     GREVNLRDLQALMFAAGATGAMVGNYLTTEGRPAEEDLRMLRDLGLASREN"
     gene            complement(2583..>3380)
                     /locus_tag="VF357_06180"
     CDS             complement(2583..>3380)
                     /locus_tag="VF357_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX7523769.1"
                     /translation="GSASRSDSAPRRKYRRVENKVTTIIAEVEKARKGIAVCAVLFLA
                     LAGLCFAFSERVLLVLVRLLGKKLVSYSPEEGLIALASLSLYCAFVLTLPVAGYLLWR
                     RLILPRVPAWRWWGGPVIAIASVLFACGAILGYFVILPAGIGFLVGFETRDVRALISA
                     KKFISFCGTMLIALGLSFEAPLLAFFLAKLGWLKPAFFRNRWRHAVLICTVLAAVITP
                     TPDVYNMTLMAIPLLGLYFVSFLVVHVVALGRVTAPVPKDGDDSGER"
BASE COUNT          634 a         1056 c         1201 g          489 t
ORIGIN      
        1 tgagcggcct cggacgatac tgcaggtgtg cgggcgccgg gcgcagaccc gcgatccccg
       61 gggaatcgga cgcttcgcac agggggcgcg cctcctccaa cgcgatctcg aagcctgcgt
      121 cgtaggcgta gaagaagaac aggtgcccgg acgccacgcg gatgccgctc acgtcgaacc
      181 tccgatcagg gatgggaaga ggttgtacag gaagtggagg gccgtcggga cgtacacgtt
      241 ccccgacctt tttcttcccc atccgaggag gaccgcgggg aagaaggtca gcaggcggaa
      301 gggggacggg gcgatcacga agtggccgat ggcgaaaagc agggcgacgg gcaacaccgg
      361 ttcccacccc gcctcctcga aggcatcgta caggtacccc cggaagaaga cctcttcggg
      421 cagggcgacc aggagcagat ggtgggcggc catcgccgcc gtcagaggaa cggtcccgtg
      481 gtacggggag atcgccgggg gcaggggaag ccgcacgaac aggaagaagg cgatcgcccc
      541 cgcgccggcg agcagcgcga agaacgcaac gctcctgccg gggtccgtcg cgcgagccca
      601 tgccgggaaa ccgcggcgcc aataccgggg gaggggggca tagagaaaga gggccgcggc
      661 gaggagcgac ggagagaaaa agggcgtcga ggggagggag agggacaaac ggaccgcggc
      721 gatcacggcg acgaagacca gcagcggttt caccgggaac ggtttcaggt cgtccacggc
      781 tcccccctca tcccgccggg cggatccggg tgaacaggtc ggggaagcgg tcgacggtga
      841 agtcgggcgg ggactcggcg aacccggaag ctcccaggcc gtaggtgacg ccgcaggtga
      901 tggtcccggc cgcccttccc gcctgcacgt cgttgcggga atccccgatc atgatcgctt
      961 cctccgcctc gactcgcgtc tcccggagga tgtggagcaa cccctcggga tcgggctttt
     1021 tggtgccgaa gctgtccccc ccctgcacct cgaagaagta cccgtcgagg gagagacccg
     1081 aaaggatctc ccgcgtcatc gccactccct tgttcgtcag cacggccatc cggtagatcc
     1141 cagcccattt ccgcagcgcc ccgaccaccc ccggatatac gcgggtggtg tccagcagat
     1201 gtctccggta atacgccagg aaccggtcga ggacttccgg gagcaggtcg gctttttcct
     1261 gaggaagagc gcgcaggatc agcgcggacg ccccgtctcc gacgtacgaa tagatcgtcg
     1321 gatgcggcag gggcgggaga tcgaacgact cgagcgccac gttcacggcg attgcgagat
     1381 ccaccttcga atccaccagc gtcccgtcca agtcgaagat cagcagccgc gccgcgtatt
     1441 ccatcctgct attgtaatga aaatgcggtg tctcgggggt ggtccccgcg tcgcgcgctc
     1501 cgcggcgcgg gcgcagtaaa ccttttggac cttccgtggt tgacagcccg cgacccgagc
     1561 accgagaatg aaaatgtttg atattccttg aggagccggt ccatggaacg attctgggag
     1621 cgcgcccgca cgaaggcgat gtcgggggag gggatcggca gggaggatgc ccttgcggtc
     1681 ctttccctcg gcaacgacgc cctgtggcgg ctgcttgacg tcaccgagtc cgtccgccgc
     1741 cggttcaagg gggacgggat ccgtctttgc tccatcgtca atgcgaagtc cgggctctgc
     1801 tcggaggatt gcgccttctg cgcgcagtcc cgccaatccg ccgccggaat cgggaaatac
     1861 cccctcatgt ccggggagga gatcttccgg gaagcggcgg ccgcgaagga gcgcggggcc
     1921 cgcgaattct cgatcgtcgc ctccggtctc gcgatgcgga accgggagga actgacccgc
     1981 gtcggggacg cggtggaccg gatccggacg gagttggggc tggaaacgtg cgtgagcctc
     2041 gggaccctcc ccccgtcgga cgtggaatat ctcttgtcgc ggggacttcg gtcggttcac
     2101 cacaatctgg agacgtcccg ctccttcttt ccgaaggtgt gcaccaccca cgactacgag
     2161 gaggacgtgg cggcggtgcg ggcggcgaag gcggcgggag cgtgggtctg ctgcggcggg
     2221 atcttcggac tgggcgagtc cccggaagac cgcgtggagc tggccttgtc gctgcgggaa
     2281 ctcagggtcg actccatccc cgtcaacttc ctgaaccccg tcgccggcac gccgttggag
     2341 gggcgccggg aattgtcccc gatggactgc cttcgcatca tcgcgatgct gcgcctgacg
     2401 aacccgacgc gggagatcat cgtgtgcggg gggcgcgagg tgaacctgcg cgaccttcag
     2461 gcgctgatgt tcgccgcggg ggccaccggg gcgatggtgg ggaactacct cacgacggag
     2521 gggcgcccgg cggaggagga cctgcggatg ctgcgcgacc tcgggctcgc ttcaagggaa
     2581 aactaacgct ctccgctgtc gtcgccgtcc ttcggtaccg gggccgtgac gcgtccgagg
     2641 gcgaccacgt ggacgacgag gaaagagacg aagtagagcc ccagtagcgg gatcgccatc
     2701 agcgtcatgt tgtagacgtc gggcgtgggc gtgatgaccg cggcgaggac cgtgcagatc
     2761 aggacggcgt gccgccaccg gttccggaag aatgccggct tcagccatcc gagcttcgca
     2821 aggaagaagg cgaggagcgg cgcctcgaag gagagcccga gggcgatcag catcgtcccg
     2881 cagaaggaga tgaacttctt cgcggagatg agcgcccgaa cgtcgcgagt ctcgaacccc
     2941 acgaggaagc cgatccccgc cggcaggatc acgaagtagc ccagaatggc accgcatgcg
     3001 aagaggaccg acgcgatggc gatgaccggg ccgccccacc accgccacgc cgggacgcgg
     3061 gggaggatca gccgtcgcca aagcaggtac cccgcgacgg gcagcgtcag gacaaaggcg
     3121 cagtacaggg agagagaggc aagggcgatg agcccctcct ccggggagta ggagacaagc
     3181 ttcttcccca gcaggcggac gagcaccagc agcacccgct ccgagaaggc gaaacagagt
     3241 ccggcgaggg cgaggaagag gacggcgcac accgcgatcc ccttgcgggc cttctcgacc
     3301 tcggcgatga tcgtggtgac cttgttttcc accctgcggt atttccttcg tggcgcggag
     3361 tccgatcggc ttgcggaacc
//
LOCUS       DASVJL010000292         4596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_234181, whole genome shotgun sequence.
ACCESSION   DASVJL010000292 DASVJL010000000
VERSION     DASVJL010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234181"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..854
                     /locus_tag="VF357_06185"
     CDS             54..854
                     /locus_tag="VF357_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX7523770.1"
                     /translation="MKPTRAAIAAAMLFCSILIGAGEETLAGHPLGTEDAGTQGKGNV
                     EVEFNLERQHGNDGTKILSLGNIITMGIAPKIDLAVGYAYDFTKAEDGTKSRAMGPVV
                     ATLKTAIIEGKDRLPTFGVKAGFSLPTSEGDQAALLATAIAQWSFEPMTIFANVGANI
                     GTHLAGNAEKNSSIRASVAGTYDLRKEWSLLSELLWEKQTSPSAPSTSEWLIGAKKEI
                     AGTLNVNGGFRWGLNGDSPHVTYLLGFTLGFRGDPSAQAAAAPAGDKK"
     gene            874..1917
                     /locus_tag="VF357_06190"
     CDS             874..1917
                     /locus_tag="VF357_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX7523771.1"
                     /translation="MHMADALLSPAVGATLWAGTIATIGYASKKLKEHLDDRKIPLMG
                     VLGAFIFASQMINFTIPGTGSSGHLGGGMILAALLGPHAALLVMASVLTVQALFFADG
                     GLLALGCNIWNLGIYPCFVAYPLIYKPMAGDGRSARRILVASLASGIVALQLGAFSVV
                     LQTLLSGKTALPFTAFLLMMQPIHLAIGIVEGLVTAGVINYVRVVRPEILDTFDSTAP
                     LATGISLRNVLIGFVALAIVTGGALSWFASAHPDGLEWSIAKVTGKGQLPGQDHGIPA
                     ALKSVQEKTAFLPGYGFKPPGNEPRAKEEAPNRPAADAGTSLSGLIGGVLVLGLALGI
                     GWAIRAFRGKNIA"
     gene            1944..2753
                     /gene="cbiQ"
                     /locus_tag="VF357_06195"
     CDS             1944..2753
                     /gene="cbiQ"
                     /locus_tag="VF357_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HEX7523772.1"
                     /translation="MSLEAAYSGIGRLDRLSYRDTPVHRLDPRAKVIATLLFVAVVVS
                     FPKYEVLSLLPFFLFPVLVGALGDIPAGFIAKKVVAVSPFAVFIGIFNPIFDPSTVAI
                     VPGVSISAGWVSFASILVKFALTISAALLLIATTSFPGICRALRRLGLPALFVSQLLF
                     LYRYLFVLMEEAMRVARARDMRSFGKRGSGVRVFIRIVGTLFLRTVERAERIYGAMLA
                     RGFRGDVPSMRRETLRSSDVFFVLAAGCFFALCRFVPVPQMIGRLVRGFAG"
     gene            2750..3508
                     /locus_tag="VF357_06200"
     CDS             2750..3508
                     /locus_tag="VF357_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523773.1"
                     /translation="MSHHLLEFKDVRFRYPDGTEALKGVSFLITHGESVGIVGANGAG
                     KSTVLLHMNGHLLPTSGAVTVGEVPLRKETRPEIRRKVGVVFQNPDDQLFMPTVFDDV
                     AFGPLNLGLPPDRVRERVHEALRQVNALALVDKPPHHLSGGQKSAVAIATVIAMKPDI
                     LVMDEPAASLDPKSRRSVIGLLNSFTHSKIVASHDLDLILDVCARCLVIRDGAIVADG
                     PSPEILSDATLLAENNLELPLTLQGRRRLEGTRP"
     gene            3522..>4596
                     /gene="larA"
                     /locus_tag="VF357_06205"
     CDS             3522..>4596
                     /gene="larA"
                     /locus_tag="VF357_06205"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEX7523774.1"
                     /translation="MRQSHVVLHYGRGSLPLPAGFFPRVNLLAAKPQPLPPSEEGHIG
                     LRIARPAGSLLLSRLARRGDRVAIAISDITRYSATEIILPFLLRETDAAGIPRDRVTL
                     FVARGTHRAMTESEVRQAVGPEIDSGIRVEQSDPDGDLATLGTTSRGTKVLVSRRLME
                     HDRIVLTGTISFHYFAGFGGGRKALVPGCAGRETAHQTHFRIFRTDGSGKHPMARAGV
                     LAGNPVHEDIVEAVSMASPTFLVNTLLTPGKRIFDLVAGHWQKAHEEGCALYAKHFRV
                     RIAKRYPLVIASAGGFPKDINLIQSHKALDNAFLATEPGGVLILLAECPDGFGSPHFF
                     PWFRNKDPDALERELRANYQIYGQ"
BASE COUNT          754 a         1560 c         1480 g          802 t
ORIGIN      
        1 gcttggcagg cgtaagtggc atcagaatcc ggaagggaaa gggcactctc ggcatgaaac
       61 cgaccagggc ggctattgct gccgccatgc tcttctgcag cattctgatc ggggcagggg
      121 aagagacgct cgccggtcac cccctcggca cggaagacgc gggaacgcag ggaaaaggga
      181 acgtcgaagt cgagttcaac ctcgaacggc agcacggcaa cgatggaacg aagatcctct
      241 ccctcggaaa catcattacg atggggatcg ccccgaaaat cgacctcgcc gtcggctatg
      301 cctacgactt cacgaaagcg gaagacggca cgaagtcccg ggcgatgggt cccgtcgtgg
      361 cgaccctaaa gacggccatc atcgaaggga aggaccggct cccgacgttc ggggtcaagg
      421 cgggattctc gctcccgacc tccgaagggg atcaggccgc gcttctggcg accgccatcg
      481 cccaatggtc gttcgaaccg atgacgatct tcgccaacgt gggggccaac atcggaacac
      541 acctggccgg gaacgccgaa aaaaacagtt cgatccgtgc gagcgtggcc ggcacctatg
      601 atctccggaa ggaatggtcc ctcctttccg agttgctctg ggagaaacag acctcccctt
      661 ccgccccgtc caccagcgaa tggctgatcg gagcgaagaa ggagatcgcc ggaacgttga
      721 acgtcaacgg cgggttccgg tgggggctga acggcgattc gccgcacgtg acctacctgc
      781 tcggattcac gctcggtttc cgtggagatc cttccgccca ggccgcggca gcgccggcag
      841 gcgacaaaaa gtaaaaagta aggagacatg agcatgcaca tggcggacgc gttgctctcc
      901 cccgcagtgg gggccacgct ctgggcaggg acgatcgcta cgatcggtta cgcgtcgaag
      961 aagctgaagg aacatctcga cgaccggaag attccactga tgggggtgct gggcgccttc
     1021 atcttcgcct cccagatgat caacttcacg atccccggga ccgggtcgag cgggcacctc
     1081 gggggcggga tgatcctcgc ggccctcctc ggaccgcacg ccgccctcct ggtcatggcc
     1141 tcggtgttga cggtccaggc gctcttcttc gccgacggcg ggctgctggc gctggggtgc
     1201 aacatctgga acctcgggat ctacccgtgc ttcgtcgcct acccgctgat ctacaagccg
     1261 atggcggggg acggccggag cgcccgacgc atccttgtcg cgtcgttggc gagcgggatc
     1321 gtcgcgctcc agctgggcgc cttctcggtc gtcttgcaga cgctcctgtc gggcaagacc
     1381 gcgctcccgt tcaccgcgtt ccttctgatg atgcagccga tccatctggc catcgggatc
     1441 gtcgagggac tcgtcacggc gggcgtgatc aactacgtcc gggtggtccg tcccgagatc
     1501 ctcgacacct tcgactccac cgcgccgctt gcgacgggga tctccctccg gaacgtactg
     1561 atcggcttcg tcgctctcgc gatcgttacg ggaggggcgc tgtcgtggtt cgcctcggcc
     1621 caccccgacg ggctcgagtg gtcgatcgca aaggtgaccg gcaagggaca actccccgga
     1681 caggatcacg gcatcccggc ggcattgaag agcgtgcagg agaaaaccgc cttcctgccc
     1741 ggttacggtt tcaaaccgcc ggggaacgag ccgagggcga aggaggaggc gccgaaccgg
     1801 ccggccgccg acgcgggcac ctcgctctcg ggcctgatcg gcggcgtcct ggtcctcggg
     1861 ctcgctctcg gaatcggatg ggcgatccgg gcgttccgtg gaaagaacat cgcctgaacg
     1921 ggatataatc gacccggatt cccatgagcc tcgaagccgc atattccggc atcggccggc
     1981 tggaccgcct ctcctaccgg gacacgcccg tccaccggct ggacccgcgc gccaaggtga
     2041 tcgcaacgtt gctgttcgtg gccgtggtcg tctccttccc gaagtatgaa gttctgtcgc
     2101 tcctcccctt cttccttttc ccggtcctgg tcggggccct cggcgacatc ccggccggtt
     2161 tcatcgcgaa aaaagttgtc gccgtctccc ctttcgcggt gttcatcggg atcttcaacc
     2221 ccatcttcga cccttcgacg gtcgcgatcg tacccggcgt ctcgatctcc gccggctggg
     2281 tttccttcgc gtcgatcctc gtgaagttcg cgctgacgat cagcgccgcc ctcctgctga
     2341 tcgcgacgac ctccttcccc ggcatctgcc gcgcgttgcg acgactgggg ctcccggccc
     2401 tcttcgtttc ccagctcctc ttcctgtatc ggtatctctt cgtcctcatg gaggaagcga
     2461 tgcgggtcgc ccgcgcgcgg gacatgcgct ccttcgggaa gaggggctcc ggcgtgcggg
     2521 tctttatccg catcgtgggc acgctcttcc tgcgcaccgt ggaacgggcg gagcggatct
     2581 acggggcgat gctcgcgcgg ggtttccggg gagacgtccc ttcgatgcgg cgcgagacgt
     2641 tgcgatcttc cgacgtcttc ttcgtgctgg cggcgggctg cttcttcgcc ctgtgccggt
     2701 tcgtccccgt gccgcagatg atcgggcgcc tggttcgggg gttcgccgga tgagccatca
     2761 cctcctcgag ttcaaggacg tccgcttccg gtacccggac ggcacggagg cgctgaaagg
     2821 ggtttcgttc ctgatcaccc acggcgagtc ggtcggcatc gtcggcgcga acggcgcggg
     2881 gaaatcgacg gtgctcctcc acatgaacgg tcacctgctt ccgacgtcag gcgccgtgac
     2941 ggtcggggag gttccccttc ggaaggagac ccggccggag atccgcagga aggtcggcgt
     3001 cgtcttccag aaccccgacg accagctctt catgccgacc gtcttcgacg acgtcgcctt
     3061 cggccccctg aacctcgggc tgccgcccga ccgggtccgg gagcgggtgc acgaggcgct
     3121 tcggcaggtg aacgccctcg cgctcgtgga caagccgccc caccaccttt cggggggaca
     3181 gaaaagcgcc gtggcgatcg ccacggtcat cgcgatgaaa cccgacatcc tcgtcatgga
     3241 cgaaccggcc gccagcctcg acccgaagtc ccggcgttcc gtcatcggct tgctcaactc
     3301 cttcacgcat tcgaagatcg tcgcctcgca cgacctcgac ctcatcctcg acgtctgcgc
     3361 ccgatgcctc gtgatccggg acggcgcgat cgtcgccgac ggcccctcac cggaaatcct
     3421 ctccgacgcg acgctgctcg cggagaacaa cctggagctt cccctgactc tccaggggcg
     3481 gcgccgcctg gaaggaacgc gcccatgagc gctccacggc catgaggcaa tcccacgtcg
     3541 tcctccatta cgggcgaggg tccctgccgc tcccggccgg gttcttccct cgggtgaacc
     3601 tcctcgcggc gaagccccag ccgcttcctc cgtcggaaga agggcacatc gggcttcgga
     3661 tcgcccgccc ggcaggctcc ctcctgctct cccggctggc ccgccgggga gaccgggtcg
     3721 cgatcgccat ttcggacatc acccgctaca gcgctaccga aataattctt ccgttcctcc
     3781 tgcgcgaaac cgacgcggcg ggcataccgc gggaccgcgt gaccctcttc gtcgcccggg
     3841 ggacccaccg cgcaatgacg gaatccgagg tgcggcaagc ggtcgggccg gagatcgact
     3901 cggggatccg cgtggaacag tcggacccgg acggggacct cgcaacgctc gggacgacct
     3961 cgcgcggcac gaaggttctc gtctcccggc ggctgatgga gcacgaccgg atcgtcctga
     4021 cggggacgat ctcgttccac tacttcgcgg ggttcggcgg cggcaggaag gcgctggtgc
     4081 ccggatgcgc ggggagggag acggcgcacc agacgcactt ccgcattttc cggaccgacg
     4141 gctccgggaa gcacccgatg gcgcgcgcgg gggtcctggc ggggaacccg gtccacgagg
     4201 acatcgtcga ggccgtttcg atggcctcgc cgaccttcct ggtgaacacg ctgctcaccc
     4261 cgggaaagcg gatcttcgac ctggtggccg ggcactggca gaaggcccac gaggaggggt
     4321 gcgcgctgta cgcgaagcat ttccgggtgc ggatcgcgaa gcggtatccc ctggtgatcg
     4381 cctccgcggg cggtttcccg aaggacatca atctgatcca gtcccacaag gcgctggaca
     4441 acgcgttcct cgcgactgag cccggcgggg tgctgatcct gctcgccgag tgcccggacg
     4501 gcttcggaag cccgcacttt tttccctggt tccggaacaa ggatcccgac gcgctggagc
     4561 gggagctgcg cgcgaactac cagatctacg gacaga
//
LOCUS       DASVJL010000293         5066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_516627, whole genome shotgun sequence.
ACCESSION   DASVJL010000293 DASVJL010000000
VERSION     DASVJL010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516627"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VF357_06210"
     CDS             <1..486
                     /locus_tag="VF357_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX7523775.1"
                     /translation="DWDHPLTDDDLAAACAHVPQGALLTVILDCCHSGTGLRDFAPDR
                     HRFLPHPEETHRNDLNLDRRPRRQNPGRRFGVSVTRTNAVLIAACRDDQTSADAWIDG
                     GYHGAHTFYLCRALADGARDLTCRALVSAAGTALSRAGFDQVPQLEGPAKLLELPFSS
                     T"
     gene            762..1025
                     /locus_tag="VF357_06215"
     CDS             762..1025
                     /locus_tag="VF357_06215"
                     /inference="COORDINATES: protein motif:HMM:NF039814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5647 family protein"
                     /protein_id="HEX7523776.1"
                     /translation="MTKFERLNSVLGYEFDRYVMEHPDFADKIPPGAIVVLQLEGRDA
                     FNKWARNIAELHRGKDEPILFVQVKGLRPPKSRLIRPVIAKSA"
     gene            1192..1686
                     /locus_tag="VF357_06220"
     CDS             1192..1686
                     /locus_tag="VF357_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HEX7523777.1"
                     /translation="MDPKWYLVKTKALNETRVHKRLTGAGYEVLFPKIQKKSSRHRGH
                     FEMRPLFPTYLFVRFVGDELKTIKYTHGVARVICFGLEPHEVDEEIIAAVRSRMNEEG
                     IVKLEPKKVVLSPGQRVKIGEGPFAGFDAVFVEELPDRERVVLLLNAVSSYRLTVQKD
                     SLEP"
     gene            1873..4986
                     /locus_tag="VF357_06225"
     CDS             1873..4986
                     /locus_tag="VF357_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX7523778.1"
                     /translation="MPERHLRLPAVLFLLFAFLGGAALAAENSMSGSAPSLPADLSPE
                     QAEMARKYLESNPEARKALEAQQQKEQESREEGKPGDENTEGGKKDLAPGAEGAAVAP
                     KEERFAAPRYDWRKSPYVSRLFGSRLKDEEKRQLVHFGHDLFAPRAGDTAILENMPAA
                     PGYVIGPGDEVVVRMWGRMEGTQRLTVDRDGKIFFPKLGSFYVAGKTFEELKAFLMGK
                     VSGIKEVRSDVSLGRMKGIRVAVIGEVRVPGWYNVSSLHTALQALSLASGVKDIGSLR
                     RIELRRGGKAVETIDLYDFLLKGDGRSDLRLLQGDTIFVPVVGKLVVIAGEVRRPAIY
                     ELRDEKPLLEMVKVAGGFSPSAWKQRVLVERLKGNTSRVVLDASVEDLENGKATMELS
                     DGDIVRVFPILPVDVNGVTLEGNVNRPGKYELKPGMTVGSLLKDAMDFLPETYFDYAL
                     LTRLVPPDLHKEVIPVNLREIVLEKKPEADVALQGRDKLTVFNRSAFRDLPKATISGE
                     VRLTRQLPGKPPKADGLFPAGQGLEKPLKTESPLPAGPGREKPLKTEGSLAPVPALEP
                     SPKTEGQVTTGKAVLGMKSEKDFLLSKLAAESRVDNNVLTIEIQAGMRVADLVRMAGG
                     LTRLAYLERAEVVRVDENRTYRTIYFHLGKAIAGDPGENLLLSNEDHVQIHSIMETRF
                     KKTVLAAGEVNNAGEYVLTDGMRLSDLLFKAGGFRESAYTKEAEVIRREISPRGDLVR
                     THAFVVFPEKALRGEADADIPLKEHDLLVVRQISDWSEKIQVVLAGEVQFPGIYAAHK
                     RERLSSVIERAGGFTNSAYLKAAQFTRLSTQKTQQEAIDKLIDDLEVEVAQKAQQVSG
                     ALDKEDVEANKQLVEARRALIAQLKKATAKGRIVIRLTSADQMKGSTSDILLEDGDRL
                     EIPKKTNVVNVVGRVYNPTGVVYDPANDTVGHYLKTVGGPTESADRDHVFLLKSNGSV
                     VTRENADAGFFSTGRQGLLSTRVDPGDSIVVPEKLIETRLMKDVKDITQILYQIAVSV
                     GVLILAF"
BASE COUNT          981 a         1651 c         1586 g          848 t
ORIGIN      
        1 gactgggatc acccgctcac ggacgacgac ctcgccgccg cctgcgccca cgttccgcaa
       61 ggggccctcc tcaccgtcat cctcgactgc tgccactccg gcaccggcct gcgcgacttc
      121 gcccccgacc gccaccgctt cctcccgcac ccggaggaga cgcaccggaa cgatctgaac
      181 ctcgaccgcc ggccgaggcg acaaaatccg ggccgtcggt tcggcgtcag cgtcacgcgc
      241 accaatgccg tgctgatcgc cgcctgccgg gacgaccaga cctcggccga cgcctggatc
      301 gacggcggct accacggcgc ccatacgttt tatctctgcc gcgctctcgc cgacggagcc
      361 cgcgatctca cctgccgcgc cctcgtctcc gcggccggga ccgccctctc ccgcgccggc
      421 ttcgaccagg tcccccaact cgaaggtcca gcaaaattgc tcgagctgcc attctcctca
      481 acatagtgta cggcggaacg aacaggtgcc gcgccaagtc cgatacatcc ggaggctgtc
      541 ggaaaatgcc agaaaaaggc atgtccatat tccaagtgga atccgattgt acgatatgat
      601 ggttcccatg gttatccaca caaggatgtt tttcgccgat gcggagagtc gcgaaaagtg
      661 ccgttgaaca tgtcgttcaa ggaggcactt gtcttggcgg atcaggatat cggggaccgg
      721 tgggaaaagt accttcaacg gtttctgcgg ggggattggc cgtgacgaaa tttgagcgat
      781 tgaattccgt tctcgggtac gagtttgatc ggtacgttat ggaacatccg gattttgcgg
      841 ataagattcc gccgggggcg atcgtagtcc tccaactgga agggagagat gctttcaaca
      901 aatgggcccg gaatatcgcc gagcttcacc gtggtaaaga tgagcccatc ctgttcgttc
      961 aggtaaaggg gttgcgtccc cccaagtccc gcctcatccg tcccgtcatc gccaagtccg
     1021 cctgaaccct cacgcccggt tttgcacctg cccctccccc cgaccccttg ccgttaattc
     1081 gcggctttga tatggttcac ccataatccg gcgtacgccc catttcaatg aaactcgcat
     1141 tccgtgcgcg tgcggatctc cccgatggcg atatccgcag gagggcagtg cttggacccg
     1201 aagtggtacc tggtcaagac gaaagcgctc aatgaaacgc gagtccacaa gcgcctgacc
     1261 ggggccggct acgaagtcct cttcccgaaa atccagaaga aatcgagccg ccaccgcggc
     1321 cacttcgaga tgcgccctct cttcccgacg tacctcttcg tccggttcgt cggcgacgaa
     1381 ctgaaaacga tcaaatatac ccacggcgtc gcccgggtga tctgcttcgg cctggagccc
     1441 cacgaagtgg atgaggagat catcgccgcc gtccggtcgc ggatgaacga ggaggggatc
     1501 gtcaagctgg agccgaagaa ggtcgtcctg tcccccggcc agcgcgtcaa gatcggcgag
     1561 gggcctttcg cgggcttcga cgcggtcttc gtcgaggagc tccccgaccg cgagcgcgtt
     1621 gtcctgctcc tgaacgccgt ctccagctac cgattgacgg tccagaaaga ttcgctcgag
     1681 ccttagtgct gcgtttccaa tgggttgagg aggtccccga atttgccggc cgtcggcttc
     1741 aactctggta ccataaaacg ttgtgttgtg gaaacgttct gtcggtggta tcgccaggcg
     1801 gtctctccct gttttcggag gccgcatggg cggtagccta cccgttttcc gctccggcgt
     1861 atcgagatga ccatgcctga acgacacctc cggttgcccg cggttctctt cctgctcttc
     1921 gcgttcctcg ggggggccgc cctcgccgcg gaaaacagca tgagcgggtc tgcgccgtcg
     1981 cttcccgccg atctctcccc ggaacaggcg gagatggcga ggaaatacct cgaatcgaat
     2041 ccggaggcgc gcaaggcgct cgaggcccag caacagaagg agcaggaatc ccgggaagag
     2101 ggaaaaccgg gggacgaaaa caccgaaggg gggaagaagg accttgcccc cggggcggaa
     2161 ggcgccgccg tagccccgaa agaggaacgg ttcgccgccc cccgctacga ctggcggaaa
     2221 tccccctacg tctcccgcct cttcgggtcc cgcctcaagg acgaggagaa gaggcagctc
     2281 gtccatttcg gccacgatct gttcgctccc cgtgccggcg acaccgcgat cctcgaaaac
     2341 atgccggcgg cccccgggta cgtcatcgga cccggcgacg aggtcgtcgt ccggatgtgg
     2401 ggccggatgg aggggaccca gcggctgacg gtcgaccgcg acgggaaaat cttcttcccg
     2461 aagctcggct ccttctatgt cgccggaaag acgttcgagg aactcaaagc ctttctcatg
     2521 gggaaggtct ccggaatcaa ggaggtccgt tccgacgtct ccctcggccg gatgaaaggc
     2581 atccgcgtcg ccgtcatcgg cgaggtccgc gtcccgggct ggtacaacgt ctcctccctc
     2641 cacaccgcgc tccaggcgct ctccctcgcc tccggggtga aagacatcgg gtctctccgc
     2701 cggatcgagc tgcgccgggg agggaaggcg gtcgagacga tcgacctcta cgatttcctg
     2761 ctgaaggggg atgggcgatc cgatctccgt ctcctccagg gggacacgat cttcgtcccc
     2821 gtggtgggga agctggtcgt catcgccggc gaagtgcgcc ggcccgccat ctacgagctg
     2881 cgcgacgaga agccgctgct ggaaatggtc aaggtcgcgg gcgggttctc tccctccgcc
     2941 tggaagcagc gcgtcctggt ggagcggctc aaggggaaca cctcccgcgt cgtcctcgac
     3001 gcgagcgtcg aggacctgga aaacgggaag gcgacgatgg agctctccga cggcgatatc
     3061 gtccgcgtct tccccattct cccggtggac gtcaacgggg tcacgctgga ggggaacgtc
     3121 aaccgccccg ggaagtacga gctcaagccc gggatgaccg tgggctcgct cctcaaggac
     3181 gcgatggatt tcctccccga aacgtacttc gactacgcgc tcctgacccg actggtccct
     3241 ccggacctgc acaaggaggt catccccgta aacctccggg agatcgtgct ggagaagaag
     3301 ccggaggccg acgtcgccct gcaggggcgg gacaagctca cggtcttcaa ccgttccgcc
     3361 ttccgcgacc tgcccaaggc gaccatctcc ggggaggtgc ggttgacgcg ccagctcccc
     3421 ggaaagccgc cgaaggccga cggtttgttc ccggcgggac aagggctgga aaaacctctc
     3481 aagacggaga gtccgctccc ggccgggccg gggcgggaga aaccgctaaa gacggagggc
     3541 tcgctcgcgc cggtgccggc gctggaaccg tcgccgaaga cggagggcca ggtcacgacc
     3601 gggaaagcgg tcctggggat gaaaagcgaa aaggacttcc tcctctccaa gctggccgcg
     3661 gaatcgaggg tcgacaacaa cgtcctcacc atcgagatcc aggcggggat gcgcgtggcc
     3721 gacctggtcc ggatggcggg gggcctcacg cgcctggcgt acctcgagcg cgccgaggtc
     3781 gttcgcgtcg acgagaaccg cacctaccgg acgatctact tccacctcgg aaaagcgatc
     3841 gcgggcgacc cgggggagaa cctcctcctt tcgaacgagg accacgtcca gatccactcg
     3901 atcatggaga cgcgcttcaa aaagaccgtc ctggccgcgg gagaagtgaa caacgcgggg
     3961 gaatacgtcc tcaccgacgg gatgcggctg tccgaccttc tcttcaaggc cggcgggttc
     4021 agggagagcg cctacacgaa agaggcggag gtcatccgca gggagatctc cccgcggggg
     4081 gacctggtcc gcacgcacgc attcgtcgtc ttcccggaga aggcgcttcg gggggaagcg
     4141 gatgccgata tccccctgaa agagcacgac ctcctggtcg tccggcagat ctccgactgg
     4201 tcggagaaga tccaggtagt gcttgcgggg gaggtgcagt ttcccggaat ctatgccgcc
     4261 cataaaaggg aacggttgag ctcggtcatc gagcgcgccg gggggttcac gaacagcgcg
     4321 tatctgaagg ccgcccagtt cacccgcctg tccacgcaga agacccagca ggaggcgatc
     4381 gacaagctga tcgacgacct tgaagtggag gtcgcccaga aggcgcagca ggtgagcggc
     4441 gccctcgaca aggaggacgt ggaggcgaac aagcagctcg tcgaagcccg ccgcgccctc
     4501 atcgcccagc tgaagaaagc gacggcgaag ggccgaatcg tcatccggct gacctcggcc
     4561 gaccagatga aggggtcgac ctccgacatc ctcctcgagg acggcgaccg gctcgagatc
     4621 cccaagaaga cgaacgtggt gaacgtcgtc gggcgggtct acaacccgac gggcgtcgta
     4681 tacgacccgg cgaacgacac cgtgggccac tacctcaaaa cggtgggagg ccccacggag
     4741 agcgcggacc gggaccacgt cttcctcctc aagtcgaacg gttcggtggt cacccgcgag
     4801 aacgccgacg ccggcttctt ctccaccggc cgccaaggtc tcctttcgac ccgggtggac
     4861 cccggggact cgatcgtcgt ccccgaaaag ctcatcgaaa cgcgcctgat gaaggacgtg
     4921 aaggacatca cccagatcct ctaccagatc gccgtctcgg tcggcgtgct gatcctggcg
     4981 ttctgacggg atgcatcata taatgaggca tcaagaggcg tgaggtgcgt cacatgaacc
     5041 agatcaccgt tcgagggatt gccccc
//
LOCUS       DASVJL010000294         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_46691, whole genome shotgun sequence.
ACCESSION   DASVJL010000294 DASVJL010000000
VERSION     DASVJL010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46691"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF357_06230"
     CDS             complement(<1..187)
                     /locus_tag="VF357_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523779.1"
                     /translation="MIREKVARLSARMHDGPQYPTQGAVLFVDLERREHFSRYLPVGV
                     LRSFLSGRGANMFLLHNL"
     gene            complement(184..1125)
                     /locus_tag="VF357_06235"
     CDS             complement(184..1125)
                     /locus_tag="VF357_06235"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX7523780.1"
                     /translation="MGKSSYQPKRFAVVGAGPVGVIVAAFLAKGGYDVTLCDVVPSLL
                     APALDPGILIEGTDTLQAKVARTTTRVDDLIGDPPDVVIVAVKATALSLIASTLEGFA
                     AEGRYVVSWQNGIDTERVLAKHLGAEFVLRAVVNLGCVPVGPAHVRIAFHHRPHYIQE
                     LDPRSMAAAVGISEVFTACGLNTLHTDQIQHMVWRKAVLNACMNPICAVTGKTMAEII
                     NDPILFHLVDVLIKEGVSVARANEFRLGSNFYPYCIDYIRNAGHHKPSMLQDIEAGRR
                     TEVDYINGKIIEYGAQADIPTPYNTMIRGLVKALEPK"
     gene            complement(1129..1569)
                     /locus_tag="VF357_06240"
     CDS             complement(1129..1569)
                     /locus_tag="VF357_06240"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7523781.1"
                     /translation="MKLKQLSIFLENAPGRLYEATHALGEAGINLRSLSISDSSDFGV
                     LRILVSDVGKARRVIMEKQLPARIDDVVAVEIEDVPGSLAKVLRPFQEKRVNVEYMYA
                     LAGASSGKAVMVFRFSDNDKAIEILRANKVRILDAEAFGMIENK"
BASE COUNT          277 a          525 c          555 g          302 t
ORIGIN      
        1 ggaggttatg gaggaggaac atgttcgcgc cgcggccgga gaggaaggac cgcagcaccc
       61 cgacgggaag atacctcgag aaatgctccc gccgctcgag gtcgacgaag agcacggcgc
      121 cctgcgtcgg gtattgcggg ccgtcgtgca tccgcgccga gagccgcgca actttttccc
      181 ggatcatttc ggttcgagcg ccttgacgag accgcggatc atcgtgttgt acggggtggg
      241 aatgtcggcc tgggcgccgt actcgatgat cttgccgttg atgtagtcga cctcggtccg
      301 acggcccgcc tcgatgtcct ggagcatgga cggcttgtgg tggccggcgt tacggatgta
      361 gtcgatgcag tacggataga agttcgagcc gagccggaac tcgttcgccc tggcgacgga
      421 aaccccctcc ttgatgagga cgtcgaccag gtggaagagg atcggatcgt tgatgatctc
      481 ggccatcgtc tttcccgtca cggcgcagat cgggttcatg caggcgttca ggaccgcctt
      541 gcgccagacc atgtgctgga tctggtcggt gtgcagggtg ttgagcccgc acgcggtgaa
      601 gacttcggag atcccgacgg cggcggccat cgaccggggg tcgagctcct ggatgtagtg
      661 gggacgatgg tggaaggcga tccggacgtg ggccggcccc accggaacac agccgagatt
      721 gacgacggcc cgcaggacga attcggcgcc aaggtgtttc gcgaggaccc gctcggtgtc
      781 gatgccgttc tgccagctga cgacgtaccg cccctcggcg gcgaacccct cgagggtgga
      841 ggcgatcagc gaaagggcgg tggccttgac cgccacgatc acgacgtcgg gaggatctcc
      901 gatcaggtcg tcgacgcggg tggtcgtcct ggccaccttc gcctggaggg tgtcggtccc
      961 ctcgatgagg atgccgggat ccagggccgg cgcgaggagc gacggcacca cgtcgcaaag
     1021 ggtgacatcg taccccccct tcgcgaggaa ggcggcgacg atcactccga ccgggccggc
     1081 gccgacgacc gcgaaccgct tcggctggta gctgctcttt cccatcggct acttgttctc
     1141 gatcatcccg aacgcctcgg catcgaggat ccggaccttg ttcgctcgca ggatttcgat
     1201 cgccttgtcg ttgtcgctga accggaagac catcaccgcc ttcccggacg aggcccctgc
     1261 cagggcgtac atgtattcga cgttcacacg cttctcctgg aacggacgga gcaccttcgc
     1321 aagggacccg gggacgtcct cgatctcgac cgctacgacg tcgtcgatgc gggccggaag
     1381 ctgtttctcc atgatcaccc gccgggcctt gccgacgtcg gacacgagga tccgaaggac
     1441 cccgaagtcg gagctgtcgc tgatgctgag ggaccgcagg ttgatccccg cctcgcccag
     1501 ggcatgggtc gcctcgtaca gccgccccgg cgcgttctcc agaaagatcg acagttgctt
     1561 caacttcatc ggaacctccc gtgcgtcatg cgctccgcca tcccggctca tgagcgtggt
     1621 gcttccgggc gcagcgtgtt gccattcgcc ctctcagcc
//
LOCUS       DASVJL010000295         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1266916, whole genome shotgun sequence.
ACCESSION   DASVJL010000295 DASVJL010000000
VERSION     DASVJL010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266916"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="VF357_06245"
     CDS             <1..1740
                     /locus_tag="VF357_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7523782.1"
                     /translation="TQTLIRATNVWGYRMYSRNTVRLAVESFLPMIDVWRCFDFLNYV
                     PNMAPVAEEVLRGGKIFSPAISFTESPETTDAYYLRVLKEIVSLCGGTKDIILVIKDM
                     AGVGSPARIRRLVDAFLQKYPGLIVLYHRHSTDGLVMPAMAAAAAAGAKLFDVTDDAF
                     SRFYGHPPVRPLVRYLRELGHEVRFDMKRADEASDAVRGFIRNYNRFESQFKGFSNDV
                     TAHRMPGGAFPSSFEQAEKGGFLDLMPYILKGMSYGNRIIKYFDVTPGSQITWTTWAA
                     ILQRYHKEGGEHNVKRLFSVLDRFFEAGERLEALAHADQNVLLRLYTGATDDLKNLLL
                     GKYGPLPFGWPKDWVYRSAFGDGWAEKVEAERVPSSPLSKLPDEDLEKARAAMEAEMG
                     RSVTQEEFVLYLMHPKAATDFLKFHDRNGDTTVLPTSVWFHGLRKPGDQVMITMGNKP
                     HAIRLVSIGEGVGGVKHVVLSVDNIMHVFPVELPEAAAARKTVRKAFPGNKGEIGATV
                     TGTVWRIGTKDRVLKEGGRLRKGEEVMNIEVMKTENAVKSPLAGVIRELCVKVNDRVE
                     EGQLLAVIDPGGE"
     gene            1737..2345
                     /locus_tag="VF357_06250"
     CDS             1737..2345
                     /locus_tag="VF357_06250"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX7523783.1"
                     /translation="MNRGTPDTDRFFGALRGALSPVATSEPLPPGLRSAGVLVPLRDT
                     GGRITVTLARRTERVPHHKGQICFPGGSRDPGDRDLLGTALREAEEELGIRGGDVELL
                     GAMERVPTVTGFCIQPFVARIPRDAQFHLDGFEVEETFDAPLSVFTDFSRYRAAATTF
                     LGKPYMVYFLDYDRFTIWGATARILHSLAELALRLPHSGAAV"
     gene            2408..>2695
                     /locus_tag="VF357_06255"
     CDS             2408..>2695
                     /locus_tag="VF357_06255"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX7523784.1"
                     /translation="MSPAGTRIDHDIILDIVPRGTKVLDLGCGDGSLLEKLVRTKDVR
                     GSGIEISDEGIRECIAKGLSVLQEDIDHGLKDYPDRSFDYIILNQTLQAVKK"
BASE COUNT          467 a          863 c          936 g          429 t
ORIGIN      
        1 acccagacgc tcatccgcgc cacgaacgtc tgggggtacc ggatgtattc caggaacacc
       61 gtgcggctgg cggtcgagtc attcctgccc atgatcgacg tgtggcggtg cttcgatttc
      121 ctgaactatg tcccgaacat ggcgccggtg gccgaggagg tcctgcgggg ggggaagatc
      181 ttctcgccgg ccatctcctt caccgagtcc cccgaaacca cggacgccta ctacctccgg
      241 gtgctcaagg agatcgtgtc cctgtgcggc ggcacgaagg acatcatcct ggtgatcaag
      301 gacatggccg gggtgggcag cccggcccgg atccgacgcc tcgtggacgc gtttctgcag
      361 aagtacccgg ggctgatcgt cctgtaccac cggcactcga cggacggttt ggtgatgccg
      421 gccatggccg cggcggccgc ggcgggggcg aagctcttcg acgtgaccga cgacgccttt
      481 tcccggttct acgggcaccc gccggtgcgc ccgctggtgc ggtacctgcg ggagctgggg
      541 cacgaggttc ggttcgacat gaagcgggcc gacgaggcgt ccgacgcggt ccgcgggttc
      601 atccggaact acaaccggtt cgaatcgcag ttcaagggct tctccaacga cgtcaccgcc
      661 caccggatgc cgggcggcgc cttcccctcc tccttcgagc aggcggagaa gggagggttc
      721 ctcgatctca tgccgtacat cctgaagggg atgtcgtacg ggaaccggat cataaagtat
      781 ttcgacgtga ccccgggatc gcagatcacg tggacgacgt gggcggcgat cctgcagcgg
      841 taccacaagg agggcgggga gcataacgtc aagcggctgt tcagcgtcct cgaccggttc
      901 ttcgaggcgg gggaacggct cgaggcgctt gcccatgccg atcagaacgt gctgctgcgg
      961 ctctacacgg gggccaccga cgacctgaaa aacctcctgc tcggaaagta cggccccctt
     1021 ccgttcggct ggccgaaaga ctgggtctac cggagcgcct tcggggacgg gtgggccgag
     1081 aaggtcgagg cggagcgggt accctcctcc ccgctgtcga agctgccgga cgaggacctg
     1141 gaaaaggcgc gggcggcgat ggaggccgag atgggccgct ccgtcacgca ggaggaattc
     1201 gtcctgtacc tgatgcaccc gaaggcggcg accgatttcc tcaagttcca tgaccggaac
     1261 ggcgacacca cggtgctgcc gaccagcgtc tggttccacg gcctgcgcaa gcccggggac
     1321 caggtgatga tcacgatggg caacaagccg cacgccatcc ggctggtctc catcggcgag
     1381 ggggtgggag gcgtaaaaca cgtggtgctt tccgtggaca acatcatgca cgtcttcccc
     1441 gtggagctcc ccgaggccgc cgcggcgcgc aagacggtcc ggaaggcgtt cccggggaac
     1501 aagggggaga tcggcgcgac cgtcaccgga acggtgtggc ggatcgggac gaaggaccgg
     1561 gtgctgaagg aagggggccg cctccgcaag ggcgaggagg tcatgaacat cgaggtgatg
     1621 aagaccgaga acgccgtgaa gtcccccttg gcaggcgtca tccgcgagct gtgcgtgaag
     1681 gtgaacgacc gggtcgagga gggacagctc ctcgccgtga tcgaccccgg gggcgaatga
     1741 accgcggaac tcccgatacg gaccggttct tcggcgcgct ccggggagcg ctttccccgg
     1801 tcgcgacctc cgagccgctg cccccgggat tgcgatccgc gggggttctc gtcccgttgc
     1861 gggataccgg cgggaggatc accgtgacgc tcgcccgccg gaccgagcgc gttcctcacc
     1921 acaaggggca gatctgcttt cccgggggca gtcgcgaccc gggagaccgg gacctgctgg
     1981 gcaccgcgct gcgcgaggcg gaggaggagt tggggatccg ggggggcgac gtggagttgc
     2041 tgggggcgat ggagcgggtc cccacggtca ccgggttctg catccagccg ttcgtcgccc
     2101 ggatcccgcg ggacgcgcag ttccacctgg acgggttcga ggtggaggag acgttcgacg
     2161 ctccgctgtc ggtgttcacc gacttctccc ggtaccgcgc cgccgcgacg accttcctcg
     2221 ggaaaccgta catggtgtat ttcctcgact acgaccgctt cacgatctgg ggggcgaccg
     2281 cacgcatcct ccacagcctc gcggaactcg ccttgcgcct gccgcactcc ggcgcggcgg
     2341 tttgacacgg ccttcaccgg acgcctacaa tgcctcctca cccccgcgga acaggagcgt
     2401 caccccgttg agcccggcag ggacacggat cgaccacgac atcatcctcg acatcgtccc
     2461 ccgcggcacg aaggtgctcg acctcgggtg cggcgacgga tcgctcctcg agaagctggt
     2521 gcgcacgaag gatgtccgcg gcagcggcat cgaaatctcg gacgagggga tccgggagtg
     2581 catcgccaag gggctttccg ttctgcagga ggacatcgac cacgggttga aggattaccc
     2641 cgaccggtcg ttcgactaca tcatcctcaa ccagacgctg caggcggtga agaag
//
LOCUS       DASVJL010000296         4773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_609797, whole genome shotgun sequence.
ACCESSION   DASVJL010000296 DASVJL010000000
VERSION     DASVJL010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609797"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /gene="secY"
                     /locus_tag="VF357_06260"
     CDS             complement(<1..964)
                     /gene="secY"
                     /locus_tag="VF357_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX7523785.1"
                     /translation="MFEGFQNITRVPELKRRILVTGLLLIVYRIGIHIPTPGIDNLAL
                     KAVFESQAGTLFGLIDMFSGGALARFSIFTLGIMPYISSSIILQLLTVVIPQLEKLSK
                     EGELGRRKITQYTRYGTIVLSVIQGMGIAIGLESVSTGAGSVVYHPGWAFRIMTVITL
                     TSGTAFLMWLGEQITERGIGNGISLIIFAGIVARFPSGLVRTVTLVRTGEMNLFAGLF
                     LLALMVAVVAVIIYFERAHRRIPVQYARRIVGRKVYGGQSTHLPLKVNTAGVIPPIFA
                     SSILLFPATIASFIKHPITRSISDALTPGSVGYELLFVAFIIFFC"
     gene            complement(969..1409)
                     /gene="rplO"
                     /locus_tag="VF357_06265"
     CDS             complement(969..1409)
                     /gene="rplO"
                     /locus_tag="VF357_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX7523786.1"
                     /translation="MKLTDLRPAKGAKSARKRVGRGEGSGLGKTSGKGNKGLKARSGG
                     GTKPGYEGGQMPLQRRLPKRGFVNVFREETAVVNVKELNRFEAGSVVDAEAFRREGLV
                     KGACPGGVKLLGNGEVTRNLTVKVDRASKSAVEKVVAAGGTVEV"
     gene            complement(1406..1600)
                     /gene="rpmD"
                     /locus_tag="VF357_06270"
     CDS             complement(1406..1600)
                     /gene="rpmD"
                     /locus_tag="VF357_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX7523787.1"
                     /translation="MSGKLRITLLRGLSGRTAYHRKVVQGLGITRLNRPVVRLDTPEI
                     RGMVEKVKFLVRMEEVGETS"
     gene            complement(1597..2097)
                     /gene="rpsE"
                     /locus_tag="VF357_06275"
     CDS             complement(1597..2097)
                     /gene="rpsE"
                     /locus_tag="VF357_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX7523788.1"
                     /translation="MKRVDPEGLELKDRVVHISRVAKVVKGGRRFSFSAVVVVGDANG
                     HVGTGLGKANEVPDAIRKAVQNAKRSLIVVPLVDGTIPHGVTGEFGASKVIIRPASPG
                     TGVIAGGGVRAVIESSGISNILTKSLGSNNPHNLVKATIEGLSQLRTAEQILAVRGPR
                     EEEATA"
     gene            complement(2138..2506)
                     /gene="rplR"
                     /locus_tag="VF357_06280"
     CDS             complement(2138..2506)
                     /gene="rplR"
                     /locus_tag="VF357_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX7523789.1"
                     /translation="MSQKNQRETARQNRKGRIRKRIFGTEQRPRLSVFRSAKHIYAQL
                     VIDSTGSTILAASTLSPDLRAEIGDLDKSDAAKKVGQWIGKKALEKNIQRVVFDRNGF
                     LYHGRIKALADGARESGLVF"
     gene            complement(2547..3080)
                     /gene="rplF"
                     /locus_tag="VF357_06285"
     CDS             complement(2547..3080)
                     /gene="rplF"
                     /locus_tag="VF357_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX7523790.1"
                     /translation="MSRIGKRPVVLPAGVKVEAVNGVLTVTGKLGQLARPIPGKLAVE
                     VKDGTATVVLLDKDAENLYGMYRTILANMVQGVTEGFLRILEIVGVGYKAESKAGALH
                     LALGYSHPVVFPLPAGVTAQVESNTVIKLSGFDKEVLGETAARVRMLRKPDVYKNKGI
                     RYRGERLIKKVGKAAGK"
     gene            complement(3117..3515)
                     /gene="rpsH"
                     /locus_tag="VF357_06290"
     CDS             complement(3117..3515)
                     /gene="rpsH"
                     /locus_tag="VF357_06290"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX7523791.1"
                     /translation="MAHNDHVSDLLARLRNAQQARFEQMEIPSTNILQGITQILKEEG
                     FIKGYRVVTEKNANRLRIALKSAPETGYAIKGIRRMSRPGRRLYVRKDEIPTVKNGFG
                     IAIVSTSRGVMTGEKAKKLSIGGELLCEIW"
     gene            complement(3533..3718)
                     /locus_tag="VF357_06295"
     CDS             complement(3533..3718)
                     /locus_tag="VF357_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX7523792.1"
                     /translation="MAKKSIIAKSKRTPKFAVRKYNRCPRCGRPRAFYRKFRLCRICL
                     RQLALRGELPGVTKASW"
     gene            complement(3739..4278)
                     /gene="rplE"
                     /locus_tag="VF357_06300"
     CDS             complement(3739..4278)
                     /gene="rplE"
                     /locus_tag="VF357_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX7523793.1"
                     /translation="MARLHDQYKAEIVPKLKEKFGYRNVMQVPRLSKVVVNMGLGDAI
                     ENVKVIETAAAEIGIITGQKPVVTKARKSIANFKLREGVPIGVMVTLRRDRMYHFLDK
                     LIAIALPRVRDFKGVSPRGFDGRGNYTLGIKEQIMFPEVNYDKIDKIRGMNITIVTTA
                     RTDEEGLELLRLMGIPFRA"
     gene            complement(4320..4661)
                     /gene="rplX"
                     /locus_tag="VF357_06305"
     CDS             complement(4320..4661)
                     /gene="rplX"
                     /locus_tag="VF357_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX7523794.1"
                     /translation="METANKTQIRKNDIVKVIAGREKGKVGRGLKIDREKGRVFVEKL
                     NLVKRHTKPGKTNPQGGIVEKEAPLSYSNVLVMCDKCNKPTRIAMAVDGAGKRSRVCK
                     RCGDVLEAKKK"
     gene            complement(4676..>4773)
                     /locus_tag="VF357_06310"
     CDS             complement(4676..>4773)
                     /locus_tag="VF357_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HEX7523795.1"
                     /translation="EPIGTRIFGPVARELRARKYMKIISLAPEVL"
BASE COUNT          781 a         1580 c         1380 g         1032 t
ORIGIN      
        1 agcagaagaa gataatgaag gcgacgaaga gcagttcgta accgacactg cccggcgtca
       61 gggcgtcgga gatcgaccgc gtgatcgggt gcttgatgaa gctggcgatc gtcgcgggga
      121 agagcagaat ggaggatgcg aagatcggcg gaatgacgcc ggccgtattt accttcagcg
      181 gcaggtgggt gctctgcccg ccgtatacct tccgcccgac gattctgcgg gcgtactgca
      241 ccgggatccg ccggtgcgcc cgctcgaaat agatgatgac ggcgacgacg gcgaccatga
      301 gcgccagcag gaagagcccg gcgaagaggt tcatttcccc ggtgcggacc agggtgaccg
      361 tgcggaccag gccgctcggg aaccgggcga cgatgccggc gaatatgatg agggagatcc
      421 cgttgccgat cccccgctcc gtgatctgtt cgcccagcca catcaggaac gccgttcccg
      481 aggtgagggt gatgaccgtc atgatccgga atgcccaacc tggatggtag acgacggacc
      541 ccgcccccgt cgaaacgctt tccagcccga tggcgatccc catcccctgg atgacggaca
      601 ggacgatcgt gccgtacctt gtgtactgcg tgattttccg ccggccgagc tctccttcct
      661 tcgagagctt ctcgagctgc gggatgacga ccgtcagcaa ctgaaggatg atcgaggaac
      721 tgatgtacgg catgatgccg agagtgaaaa tggagaagcg cgcgagcgcc cccccggaga
      781 acatgtcgat caggccgaag agggtgcccg cctggctctc gaagaccgcc ttgagcgcaa
      841 ggttgtcgat cccgggcgtg gggatatgga tcccgatgcg gtagacgatg agcaggagcc
      901 ccgtgacgag gattcgccgc ttaagctcgg ggacccgcgt tatgttctgg aagccttcga
      961 acaccggttc agacctccac ggtcccgccg gcggcgacga ctttctcgac ggcggacttg
     1021 ctggcccgat cgaccttcac ggtgagattc cgggtaacct cgccgtttcc gagaagcttg
     1081 acgccccccg gacaagcacc tttcaccaag ccttcgcggc gaaacgcctc ggcgtcgacc
     1141 acggagcccg cctcgaaccg gttcagttcc ttcacgttga cgacggccgt ctcttcccgg
     1201 aagacgttga cgaacccgcg tttcgggagc cgcctctgga gaggcatctg tccgccttcg
     1261 tacccgggct tcgtcccgcc gccgctgcgc gccttgagtc ccttgtttcc cttgccggag
     1321 gtcttcccga ggccggagcc ttcgccccgc ccgacgcgct tgcgcgcgct tttcgcccct
     1381 ttcgcggggc ggagatccgt tagtttcatg acgtctctcc cacctcttcc atccggacga
     1441 ggaacttgac cttttccacc atcccgcgga tctccggcgt gtccaaccgt acgacgggac
     1501 ggtttaaccg ggtgatcccg agtccctgaa cgaccttgcg gtggtacgcg gttctcccgc
     1561 tcaacccccg caggagggtg atgcgcaatt ttccgctcat gccgtcgcct cttcctctct
     1621 cgggccccgg accgccaaga tctgctccgc ggtccgcaac tgggaaagcc cctcgatcgt
     1681 cgcctttacg aggttgtgag ggttattgct ccccagggac ttcgtcagga tattcgagat
     1741 tccgctggat tcgatcacgg cgcggactcc accgcccgcg atgacgccgg tccccggaga
     1801 ggcgggacgt atgatgacct tcgaggctcc gaactcgccg gtcaccccgt gcgggatggt
     1861 gccatctacc agtggaacga cgatcagcga ccgcttggcg ttctgaaccg ccttgcggat
     1921 cgcgtcggga acctcgttcg ccttcccaag acccgtcccg acgtgcccgt tggcgtcacc
     1981 gacgacgacg accgcgctga aggagaagcg gcgtccgccc ttcacgacct tggcgacgcg
     2041 gcttatgtgc acgacgcggt cctttaattc cagcccttcc ggatcgactc ttttcaatcc
     2101 ttcctccccg tcccctcgcg aaagtgtgcg ctcattttca gaataccaac ccggattcac
     2161 gagctccgtc cgccagggcc ttgatccgtc catggtagag gaatccgttc cggtcgaaca
     2221 ccacccgctg gatgtttttc tccagtgcct ttttcccgat ccactgcccc accttctttg
     2281 cggcgtcgct cttgtcgagg tcgccgatct ccgcccggag atccggggag agggtggacg
     2341 ccgcaaggat ggtcgacccc gtggagtcga tcaccaattg ggcgtagatg tgcttcgcgc
     2401 tgcggaagac ggacaaccgc ggacgctgct cggtgccgaa gatccgcttg cggatccggc
     2461 ctttccggtt ctgtcgtgcg gtttcccgct ggtttttctg gctcatggcg ttcggatccc
     2521 catcgtaagg aattgcgcgt tgtccactac ttgcccgccg ccttcccgac cttcttgatc
     2581 agccgttcgc cgcggtagcg gattcccttg ttcttgtaaa cgtccggctt ccgaagcatc
     2641 cggacccggg cggccgtttc cccgagtact tccttgtcga acccgctcag cttgatcacg
     2701 gtgttcgatt cgacctgcgc ggtcacgccc gcgggcaggg ggaaaaccac cgggtgggag
     2761 taccccagcg ccaggtgaag cgcgcccgcc ttggactcgg ccttgtagcc cacaccgacg
     2821 atctccagga ttcgcaggaa gccttccgtg actccctgca ccatgttggc aaggatcgtc
     2881 cggtacatgc cgtagaggtt ctctgcgtcc ttgtcgagaa gaacgaccgt cgccgttccg
     2941 tccttcactt ccaccgccag tttccccggg atcggtcggg caagctgccc caacttcccg
     3001 gtgacggtca gcactccgtt cactgcttcc accttgactc cggcgggaag gaccacaggt
     3061 cttttaccga ttctcgacat cgtttcgctc ctcgttggaa agaacccgga agccgcctac
     3121 cagatttcgc agagcagctc gccgccgatc gaaagcttct tcgccttctc ccccgtcatg
     3181 acgccgcgcg acgtggagac gatggcgatg ccaaacccgt tcttcaccgt cgggatctcg
     3241 tccttcctga catagagtct ccgtcccggc cggctcatcc ttcgaattcc cttgatggcg
     3301 tatcccgttt ccggggcaga tttcaaggcg atgcggaggc ggttcgcgtt tttttcggtc
     3361 acgacccggt atcccttgat gaacccttcc tccttgagga tctgcgtgat tccctgaagg
     3421 atattcgtcg aggggatctc catctgttcg aagcgtgcct gctgggcatt ccgcaaccgc
     3481 gccagcaagt ccgacacgtg atcgttgtgc gccattgtca tattcttccc ccctaccagc
     3541 tcgccttggt cacgccggga agctccccgc gaagggcgag ctgcctcagg cagatccggc
     3601 acaaccggaa cttccggtaa aaagcgcgcg gcctgccgca gcgcggacac cggttgtatt
     3661 tccggacagc gaatttcggc gtccgtttcg acttggcgat gatcgatttc ttggccaacg
     3721 tctgctctcc tctcttgctt acgcccggaa gggaattccc atgagccgca gtagctcgag
     3781 accttcttca tcggtccggg ccgtggtcac gatggtgatg ttcatgccgc gtatcttgtc
     3841 gatcttgtcg taattcactt cgggaaacat gatttgttcc ttgatcccga gggtatagtt
     3901 gccgcgcccg tcgaaccctc ttggggaaac ccccttgaag tcgcgcacgc gcggaagtgc
     3961 gatcgcaatc agcttgtcga gaaagtggta catcctgtcg cgccgcagcg tgaccatgac
     4021 gccgatggga actccttccc gaagcttgaa gttcgcgatg ctcttgcgcg ccttggtcac
     4081 cacgggcttc tggccggtga tgatgccgat ctcggcggcg gcggtctcga tcaccttgac
     4141 gttttcgatc gcgtcgccga gccccatgtt cacaacgacc ttggagagcc tgggcacctg
     4201 catcacgttc cggtacccga acttctcctt cagcttggga acgatctccg ccttgtactg
     4261 gtcatgcagt ctcgccattt ccgatcaccc ccatccgccg ttccggcgga ggtatccttt
     4321 cacttctttt ttgcttcgag gacgtccccg caccgcttgc agacgcggct gcgtttcccc
     4381 gcgccgtcga ccgccatcgc gatccgggtc ggcttgttgc atttgtcgca catgacgaga
     4441 acgttggagt aggaaagcgg cgcctctttc tccacgatgc cgccctgggg gttcgtcttc
     4501 ccgggcttcg tgtgccgctt caccaggttg agtttctcga cgaagaccct ccccttctcg
     4561 cggtcgatct tgagaccccg gccgaccttc cccttctccc gtcctgcgat caccttgacg
     4621 atgtcgttct ttcggatctg cgttttgttc gcggtttcca ttccgcccct ccggctcaca
     4681 ggacttccgg cgccagggag atgatcttca tgtacttcct ggcgcgcagc tcgcgggcga
     4741 cgggtccgaa gatgcgggtc ccgatcggct cgc
//
LOCUS       DASVJL010000297         6518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1736735, whole genome shotgun sequence.
ACCESSION   DASVJL010000297 DASVJL010000000
VERSION     DASVJL010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736735"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..1932)
                     /gene="uvrC"
                     /locus_tag="VF357_06315"
     CDS             complement(253..1932)
                     /gene="uvrC"
                     /locus_tag="VF357_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX7523796.1"
                     /translation="MGLPSDWKTFPRSPGVYIMSDAGGKVLYVGKAKDLRARVRNYSV
                     PGGDGRPAIPNLIMRVREIRCIVTATEKEALLLENTLIKRHRPLFNIFFRDDKEYLLL
                     RIDRNEAFPRPELVRRAARDGATYFGPYSSARGIRETMRELLRLFPLCSCTRRKFASR
                     TRPCLNYQMGRCLGACAGLVTRERYLPVVDDAVRFLRGEYRGLLARWKTEMKALSVRM
                     RFEEAAKIRDRIAVVSKTLARQRVVRTVGGDVDAVGWHRDGPAVTAAVLYVRNGRLSD
                     AHQRHFRWEGPEEEAIDSFLLHHYGEDAFFPGEILLPFPVADRAALSAALSERAGRTV
                     EVRVPLRGERYRLVDLARRNAAEGARMRREKEEAYERLAERMADLFSLPGPPARIEGY
                     DISNLSGTEAVGSMVAFLGGKPAKKWYRKFSVRGVVGPDDFAMMEEVVGRRFAHDEDF
                     GGMPDLVLIDGGKGQLSSAAAAMRAAGREDIPLLSLAKERVRGGEKIFRERVFLPGRK
                     NPLHLPPNDPVLHLLMRIRDEAHRFAITFHRTRRTRAAFGKPRSAPGAPAE"
     gene            complement(1936..3924)
                     /gene="uvrB"
                     /locus_tag="VF357_06320"
     CDS             complement(1936..3924)
                     /gene="uvrB"
                     /locus_tag="VF357_06320"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX7523797.1"
                     /translation="MGSTFQLAAPFAPAGDQPGAIRELSDGLAHGLARQVLLGVTGSG
                     KTYTMASVIAAVDRPALVIAPNKTLAAQLYSEFKSLFPENAVEYFVSYYDYYQPEAYV
                     PHSDTYIEKDSSINEQIDKMRHSATRSVMTRGDVIVIASVSCIYGLGSPEYYGKMTVR
                     IAEGAEFPRNAFLRRLIDLQYERNDVDFHRGTFRVRGDAVELFPAYEEERVLRIEYDE
                     DRIARILHVDPLRGTRIKELSETVIFPASHYVTPDDQLERAVHGIGEELEGRLAELHS
                     QGKPLEAERLKQRTTYDLEMISQMGFCSGIENYSRHLDGRAPGQPPYTLLDYFPKGFV
                     TFLDESHVTVPQLNGMYNGDRSRKQTLVDFGFRLPSALDNRPLRFEEFNGRVGQVIFV
                     SATPAEYELRMSGGAVVEQIIRPTGLVDPEVEVRPARTQVDDLLGEIRKNAAAGGRVL
                     VTTLTKRMAEDLTEHYEGQGVRVRYLHSDIDTMERVEILRDLRLGTFDVLVGINLLRE
                     GLDLPEVSLVAILDADKEGFLRSARSLVQTFGRASRNVSGRVILYADRETGSMREAIS
                     ETGRRRERQAVYNREHGITPETIRKMIADPIGQVCEADYVTPSAGEPGFSSREELAKL
                     LRKLRKEMERAAKKLDFERAADLRDRLLALEKAELSSR"
     gene            complement(4178..5626)
                     /gene="cysS"
                     /locus_tag="VF357_06325"
     CDS             complement(4178..5626)
                     /gene="cysS"
                     /locus_tag="VF357_06325"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX7523798.1"
                     /translation="MTLSVFNTMGSRKEAFVPIAPGKVRMYVCGVTVYDLCHIGHARA
                     NVAFDIIVRYLRHTGYDVTYVRNFTDIDDKIIRRAAQEGSDYLTVSTKYIRAFHDDFD
                     RLGLLRPDVEPKATEHIPEIVALVARLVREGKAYEVGGDVYYSVKGFPGYGKLSGKNV
                     EDLRAGARVDVDERKNDPLDFALWKASKPGEPAWESPWGPGRPGWHIECSAMSMKHLG
                     ESFDIHGGGKDLVFPHHENEIAQSEAATGKPFARYWLHNGFINVNNEKMSKSLGNFFT
                     LRDVLAQVKPEVLRFFLASSHYRSPIDYSEQSLTEAKAGLDRLYRVKEKAEACRAAGA
                     APSPIPSGEEVAPLLSAPARFGEAMDDDFNTAAALGHLFDAIRALNRLSPAEPLAERE
                     RAGMFLAGYELLEPLFTVLGLLRAPSAEHFQGAGAAGRMGEGEILAGIEARRASRAAK
                     QYGEADRIRKELEAAGVLLEDGKAGTTWKYKE"
     gene            complement(5623..>6518)
                     /gene="ispF"
                     /locus_tag="VF357_06330"
     CDS             complement(5623..>6518)
                     /gene="ispF"
                     /locus_tag="VF357_06330"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX7523799.1"
                     /translation="IILVHDAVRPFATPGLFDRCAEEALRHGAAVPVIPVRDTVKTWD
                     KAAGTLVTRDRSEFLRAQTPQGFRAGILREAYALAAREGRAGTDDASLVEAAGHAVVA
                     LPGEEGNLKITLPEDLRMAAGLLSGDPDFRVGLGGDAHRLVPERELWLGGVRVDHPMG
                     LLGHSDGDVLLHAVADAIYGAMGDRDIGFHFPPGREETLGISSRSILAHARGRMAAGG
                     FGLLGLDSVVVCEAPRIAPLADALRASIAGILAVPEECVSVKGKTTEGMGFEGRGEGI
                     SAWAVALLRGTRPENGGGAAG"
BASE COUNT         1039 a         2290 c         2115 g         1074 t
ORIGIN      
        1 atgaaactcc cgtggctcat agccgaaaag ataccacagc ccccccaaaa agaaaaagca
       61 agcccccttc ggcggagccg ccgtaggagg gggggcgccc cactcccgga ctaactacac
      121 tccctggggt acccccgagt agtaagaacg gggggcacag tgaggccggt gcgcagccgt
      181 gcaggttcac cgcacggcga gccacgaacg gagccccgcc ctccgaggca acgcagcctt
      241 ggttgtgggc tgctactcgg cgggcgctcc aggtgcggaa cggggttttc cgaaggcggc
      301 gcgggtgcgg cgggtgcggt ggaaggtgat ggcgaagcgg tgggcctcgt cgcggatgcg
      361 catcagcagg tgcagcaccg ggtcgttggg gggaaggtgg agcgggtttt tccgtccggg
      421 caggaacacc cgctcccgga agatcttctc gccgccgcgg acgcgctcct tcgcgaggga
      481 aaggagaggg atgtcctccc tgcccgcggc ccgcatcgcc gccgccgcgg aggagagctg
      541 ccccttcccc ccgtcgatca ggacgaggtc cggcatccct ccgaaatcct cgtcgtgggc
      601 gaaccggcga ccgaccacct cttccatcat ggcgaagtcg tccgggccga ccaccccgcg
      661 cacggagaat ttccggtacc acttcttcgc gggcttcccg ccgaggaacg cgaccatcga
      721 ccccaccgct tccgtccccg agaggttgga gatgtcgtac ccctcgatcc gcgccggtgg
      781 acccggcagc gaaaagaggt ccgccatccg ctccgccagg cgctcgtacg cctcctcctt
      841 ctcgcggcgc atccgtgcgc cttccgcggc gttccggcgc gcaagatcca cgagccggta
      901 tcgctccccc cgcaggggaa cgcgcacctc gacggtccga cccgcccgct cggagagcgc
      961 ggcggagagc gccgcgcggt cggcgacggg gaacgggagg aggatctctc ccggaaagaa
     1021 cgcatcctcc ccgtaatggt gcagcaggaa ggagtcgatc gcctcctcct ccggcccttc
     1081 ccaccggaag tgccgctggt gcgcgtcgga gaggcgcccg ttccgcacat agaggaccgc
     1141 cgccgtcacc gcgggcccgt cgcgatgcca acccacggcg tcaacgtccc ccccgacggt
     1201 ccggacgacg cgctggcgcg ccagggtttt cgaaacgacg gcgatccggt cccggatctt
     1261 cgcggcctcc tcgaaccgca tcctcacgga gagcgccttc atctccgttt tccagcgagc
     1321 aaggagcccc cggtactccc cccggaggaa tcgcaccgcg tcgtcgacga cggggaggta
     1381 ccgctccctc gtgaccaggc cggcgcacgc gccgaggcac cttcccatct ggtagttgag
     1441 acacggccgc gtgcgggagg cgaatttccg ccgcgtgcag gagcagagcg ggaagaggcg
     1501 gagcagttcc cgcatcgttt cccggatccc ccgcgccgag gagtacgggc cgaaatacgt
     1561 cgccccgtcg cgcgccgccc tgcggaccaa ctcgggacgc gggaacgcct cgttccggtc
     1621 gatccgcagg agcagatact ccttgtcgtc acggaaaaag atgttgaaga gggggcggtg
     1681 ccgcttgatg agggtgttct cgaggaggag ggcctccttc tccgtggcgg tgacgatgca
     1741 ccggatctcc cggacacgca taatgaggtt cgggatcgcc ggccgcccgt cccccccggg
     1801 aaccgaatag ttccggacgc gggcccgcag atccttcgcc ttcccgacgt aaagcacctt
     1861 cccgccggcg tcgctcatga tgtagacgcc gggggagcgc gggaacgtct tccagtcgga
     1921 cgggagcccc atcgctcagc gggacgaaag ctccgccttc tcgagggcga ggaggcggtc
     1981 gcgcaggtcc gccgcgcgtt cgaaatcgag ttttttcgcc gcccgctcca tctccttccg
     2041 aagcttccgg aggagtttcg cgagctcttc ccgggaggag aagccgggct cccctgcgga
     2101 cggggtcacg tagtccgcct cgcacacctg cccgatcgga tccgcgatca tcttccggat
     2161 cgtctcgggg gtgatcccgt gctcccggtt gtacaccgcc tgccgttccc tccgtcggcc
     2221 cgtctccgaa atcgcctccc gcatcgaccc ggtctcccgg tccgcgtaga ggatgaccct
     2281 tccggacacg ttgcgcgaag cgcggccgaa cgtctgaacg agcgaccggg cggatcggag
     2341 aaaaccttcc ttgtccgcgt cgaggatcgc caccagggag acctccggga ggtcgagccc
     2401 ctcccggagc aggttgattc cgacgagaac atcgaacgtc ccgagccgaa ggtcccgcag
     2461 gatttcgacc cgctccatcg tgtcgatgtc ggagtggagg tagcgcacgc gtactccctg
     2521 cccctcgtaa tgctccgtca gatcctcggc catccgcttc gtgagggtcg tgaccaggac
     2581 cctcccgccc gcggcagcat tcttccggat ctcccccaga agatcgtcca cctgcgtccg
     2641 cgcggggcgg acctcgacct cggggtcgac gagccccgtg gggcggatga tctgctccac
     2701 caccgctccg ccgctcatcc gcagttcata ttccgcgggg gtcgccgaga cgaagatcac
     2761 ctgccccacc cggccgttga actcctcgaa ccggagcggc cggttgtcga gcgcggaggg
     2821 gagccggaac ccgaaatcca ccagcgtctg tttccgggac cggtcgccgt tgtacatgcc
     2881 gttcaactgg ggaacggtca cgtgggattc gtccaggaag gtgacgaacc ctttcgggaa
     2941 gtagtcgagg agcgtgtacg gcggctgtcc gggtgcccgc ccgtcgagat ggcgggagta
     3001 gttctcgatg ccggagcaga atcccatctg ggagatcatc tcgaggtcgt aggtggtcct
     3061 ctgcttcagc cgctccgcct cgagcggctt tccctgcgag tggagctccg cgagccgtcc
     3121 ttcgagctcc tccccgatcc cgtggaccgc ccgctcgagc tgatcgtccg gcgtcacgta
     3181 gtggctggcc gggaagatga ccgtctcgct caactccttg atccgcgtcc cccggagggg
     3241 atccacgtgg aggatccggg cgatccggtc ctcgtcgtac tcgatccgca ggacccgctc
     3301 ttcctcgtac gcgggaaaca gctccaccgc atctccgcgg acgcggaacg ttccgcggtg
     3361 gaagtccacg tcgttgcgct cgtactgcag gtcgatcagc cgccgcagga acgcattgcg
     3421 gggaaattcc gcgccctccg caatccggac ggtcatcttc ccgtaatact ccggggaccc
     3481 gaggccgtag atgcacgaca cggaggcgat cacgatcacg tcgccgcggg tcatcaccga
     3541 cctcgtggcg ctgtgccgca tcttgtcgat ctgttcgttg atcgaggagt ccttctcgat
     3601 gtaggtgtcc gagtggggaa cgtacgcctc gggctggtaa tagtcgtaat aggaaacaaa
     3661 atactcgacc gcgttttcgg ggaaaaggga tttgaactcg gagtacagct gcgcggcgag
     3721 cgtcttgttc ggggcgatca cgagcgcggg ccggtcgacg gcggcgatga cggaagccat
     3781 cgtgtacgtc ttccccgacc cggtgacgcc gagaaggacc tgccgcgcaa ggccgtgggc
     3841 gagcccatcg gagagttcac ggatcgcccc gggctggtcc cccgcggggg caaacggcgc
     3901 ggccaactgg aacgtggagc ccaccttagt gctccatttc acaattacga ttacatttca
     3961 cccggagtcg cctctctctc cgcggcgcct ccggattcgg tgccgtattt atgggacgca
     4021 acacttagtg gctcatcccg acgctacagg gctcgtgccc cccgttttta ctgctgcggg
     4081 ggtaccccgg cagcacgaag ctcgtatcgg ggcggggggc ttccactccg ctacgccctt
     4141 gttgcgtagg gagggtgatc ccacgaaagg gaaggcgtta ctccttgtac ttccacgtcg
     4201 tcccggcctt gccgtcctcg aggaggacgc ccgccgcctc gagttccttg cggatccggt
     4261 ccgcctctcc gtactgtttc gccgcccgcg aggctcggcg cgcctcgatc ccggcgagga
     4321 tctctccctc ccccattctc cctgcggctc ccgctccctg gaaatgctcc gcggaaggcg
     4381 cccgcagcag accgaggacc gtgaacagcg gctcgagcag ttcgtacccc gcgaggaaca
     4441 tccccgcccg ctcccgctcc gccaacggtt ccgccgggga gagccggttc agcgcgcgga
     4501 tcgcgtcgaa gaggtgcccc agcgcggcgg cggtgttgaa gtcgtcgtcc atcgcttccc
     4561 cgaaccgcgc gggggcggag agaagcggcg ctacctcttc ccccgacggg atcggggaag
     4621 gcgccgcgcc ggccgcgcgg cacgcttccg ccttctcctt gacgcggtag agccggtcca
     4681 gacccgcttt cgcctcggtg aggctttgtt cggagtagtc gatcgggctg cggtagtggc
     4741 tcgaggcaag gaagaaacgg agcacctccg gcttgacctg cgccagcacg tcccgcaagg
     4801 tgaagaagtt ccccagcgac ttgctcatct tctcgttatt gacgttgatg aacccgttgt
     4861 ggagccagta gcgggcgaac ggttttcccg tcgccgcctc ggactgcgcg atctcgttct
     4921 cgtggtgcgg gaagaccagg tcctttccgc caccgtggat gtcgaacgac tcgcccaggt
     4981 gcttcatcga catggcggag cactcgatgt gccatcccgg gcgcccgggg ccccacggac
     5041 tttcccacgc gggctcccct ggcttcgacg ccttccagag ggcgaagtcc agggggtcgt
     5101 tcttccgctc gtccacatcg acccgcgcgc ccgcccgcag gtcctcgacg ttcttcccgg
     5161 acagcttgcc gtatcccggg aaccccttca ccgagtagta gacgtcgccg ccgacctcgt
     5221 acgcctttcc ctcccggaca agacgcgcga cgagggcgac gatctcgggg atatgctccg
     5281 tggccttcgg ctcgacgtcg ggacgaagga gcccaaggcg gtcgaaatcg tcgtggaacg
     5341 cccggatgta tttcgtggag acggtgaggt aatcgctccc ctcctgcgcg gcccgacgga
     5401 tgatcttgtc gtcgatgtcg gtgaagttcc ggacgtaggt gacgtcgtac ccggtgtggc
     5461 gaaggtaccg gacgatgatg tcgaacgcca cgttggcgcg ggcgtgcccg atgtggcaca
     5521 ggtcgtacac cgtgacgccg cacacgtaca tgcggacctt gccgggagcg atcggaacga
     5581 acgcctcctt tcggctcccc atcgtgttga acaccgaaag agtcatcccg ccgcaccccc
     5641 gccgttttcc ggccgcgtcc ccctgagcag ggccaccgcc caggcggaga tcccttcgcc
     5701 ccgtccctcg aaccccatcc cttccgtggt cttccccttg acgctcacgc actcctccgg
     5761 caccgcgagg atccccgcga tggaggcgcg aagagcgtcc gccagcgggg cgatccgggg
     5821 ggcctcgcag accaccacgg agtccagccc gaggaggccg aacccccccg ccgccatcct
     5881 cccccgggcg tgcgcaagga tcgagcggct ggagatccca agggtctctt cccggccggg
     5941 cggaaaatgg aagccgatgt cccggtctcc catcgctccg tagatcgcat cggccacggc
     6001 atgcaggaga acgtccccgt cggagtgacc gaggagcccc atcgggtggt cgacccgcac
     6061 tccccccagc caaagttccc gttcgggaac gagacgatgg gcgtcccccc cgagcccgac
     6121 ccggaaatcg ggatccccgg acaggagccc cgccgccatc cgcaggtcct cgggaagcgt
     6181 gatcttgaga ttcccctcct ccccgggaag ggcgacgacc gcatggccgg ccgcctcgac
     6241 gagggaggcg tcgtccgtcc cggcgcgtcc ctcgcgcgcg gcgagggcgt acgcctcccg
     6301 aaggatcccg gcgcggaacc cctgcggggt ctgcgcgcgg aggaactcgg atcggtcccg
     6361 cgtgacgagg gtaccggccg ccttgtccca cgtcttcacc gtgtctcgga cggggatcac
     6421 cgggaccgcc gctccgtgcc ggagggcctc ttccgcgcac cggtcgaaga ggccgggggt
     6481 cgcgaagggg cggaccgcgt cgtgcacgag gatgatcg
//
LOCUS       DASVJL010000298         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_235113, whole genome shotgun sequence.
ACCESSION   DASVJL010000298 DASVJL010000000
VERSION     DASVJL010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235113"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VF357_06335"
     CDS             <1..750
                     /locus_tag="VF357_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7523800.1"
                     /translation="WMQFACGECAPCLAGAEMLCAAQRGTGVTVDGGYAEFVKAHAGF
                     VHRIPDPLDLVDSAPLLCAGITVFAPLRKAGDLAGKTVAVAGIGGLGHLAVRMAFAMG
                     ARVVAILEEEERGDLVRGLGAREIIFSRTEKVGKRLSSMGGADLILLTAASARLFEQC
                     VSGIGVNGTLVVLATVAESASIVPASLLQGQKTIRGSVTGTRDDMDAMLRFVAEHGIA
                     PVVERHPLGRVNDVVSMLRSGKVVFRAVLVP"
     gene            complement(803..>1640)
                     /locus_tag="VF357_06340"
     CDS             complement(803..>1640)
                     /locus_tag="VF357_06340"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7523801.1"
                     /translation="GKELFAQAIHDEGQRANGPFVTVNCAAIPLELAESELFGYERGA
                     FPGARTEGHPGKFEMAPRGTIFLDEINSMPLPIQAKILRVVETKRVTRINGKRETPVD
                     VRIIAASNRVLADEVATGTFRKDLFYRLNVLPLRVPALREMKEDIPALLNAFFQSCAE
                     EHGVRRKRISPEALRHLVSHDWPGNVRELKNCSEYLCFTVETDEVAEHHLPPEILARR
                     MAAPEPPAAPEEPKNLGSLERMFLIETMRRFHGNAVEAAKTLGISRSTLYRKLKKYGI
                     TP"
BASE COUNT          228 a          534 c          578 g          300 t
ORIGIN      
        1 tggatgcagt tcgcctgcgg ggagtgcgcg ccgtgtctcg ccggagccga gatgctctgc
       61 gcggcgcaaa gggggaccgg ggtcaccgtg gacggtggct acgcggagtt cgtgaaagcg
      121 cacgcggggt tcgtccaccg gatcccggat ccgctcgacc tcgtcgattc cgccccgctc
      181 ctgtgcgccg ggatcacggt gttcgcgccg ctgcgcaagg cgggggacct cgcggggaag
      241 accgtggcgg tggccggtat cggcggtctc ggtcacctgg cggtgcggat ggcgttcgcc
      301 atgggggccc gggtcgtcgc gatcctcgag gaggaggaga ggggcgatct cgtgcggggg
      361 ctcggcgccc gtgagataat cttttcgagg acggagaagg tcgggaagcg gctctcttcg
      421 atggggggcg ccgacctgat cctgctcacg gcggcttccg cccgtctttt cgagcagtgc
      481 gtttcgggca tcggcgtgaa cgggacgctc gtggtgcttg cgaccgtcgc ggagagcgcg
      541 tcgatcgtcc ccgcgtccct cctccagggg cagaagacga tccgcggctc ggtgaccggc
      601 acccgggacg acatggacgc gatgctccgg ttcgtcgcgg agcacggaat cgccccggtg
      661 gtcgagcggc atccgctggg gagggtgaac gatgtggttt cgatgctgcg ctcggggaag
      721 gtcgtcttcc gcgcggttct ggttccctga tccagagggc gggtcgggcg cgcgagtcgc
      781 cgcggtctac gtcagaaacc ccctacggag tgatcccgta tttcttgagc tttcgataaa
      841 gggtgctccg ggagatcccg agcgtcttcg cggcctcgac cgcgttcccg tggaacctgc
      901 gcatcgtctc gatgaggaac atccgctcga gcgacccgag attcttcggc tcctccggcg
      961 ccgccggcgg ctcgggcgcc gccatcctcc gggcgaggat ctcgggagga aggtggtgtt
     1021 ccgcgacctc gtccgtttcc acggtgaagc agaggtactc cgagcaattc ttcaactccc
     1081 gcacgttgcc gggccagtcg tgcgagacca ggtgccgcaa cgcttccggg gatatccgtt
     1141 tccggcggac gccgtgctcc tcggcgcacg actggaagaa ggcgttgaga agggccggga
     1201 tgtcctcctt catctcccgc agagcgggaa cccgcaatgg aaggacgttc agccggtaga
     1261 agaggtcctt gcggaacgtc ccggtcgcca cctcgtccgc cagcacccgg ttgcttgccg
     1321 cgatgatccg cacgtcgacc ggggtttccc gcttcccgtt gatccgcgtg acccgtttcg
     1381 tctccacgac gcgcaggatc ttcgcctgga tcggcagcgg catcgagttg atctcgtcga
     1441 ggaaaatggt cccccggggg gccatctcga actttcccgg gtgcccctcc gtcctggccc
     1501 ccgggaacgc accccgctcg tacccgaaca gttcgctctc ggccagctcc agcgggatcg
     1561 cggcgcagtt gaccgttacg aaagggccgt tcgcccgctg cccctcgtcg tggatcgcct
     1621 gggcgaagag ctccttcccg
//
LOCUS       DASVJL010000299         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1080333, whole genome shotgun sequence.
ACCESSION   DASVJL010000299 DASVJL010000000
VERSION     DASVJL010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080333"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VF357_06345"
     CDS             <1..727
                     /locus_tag="VF357_06345"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7523802.1"
                     /translation="LEGAIPRMIDERDFELLDRVLSSFLHASESGTPARRAAANDALR
                     SVEFLRIVDAILASPGSLEEEKGGAALLSKHGALAAEALLQKLQDEEEPGRRKILLSL
                     VLRLGEKAVPSILSRIEGQRWFFLRNLCFLLGEIGAPAGIPPLVGMLSAREPKVRREA
                     VQALGKIRMPDPDAIAVLGRTLLHEPFFSSSREDSVRIDAAIALSRIGGTEAIAFLHY
                     GKSSRKKAVREQCEALLRTRGTE"
     gene            724..>1530
                     /locus_tag="VF357_06350"
     CDS             724..>1530
                     /locus_tag="VF357_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7523803.1"
                     /translation="MKAQEVFLAILTDLGRSIQAAALYPETHQRVQEPLARLHRRVWN
                     EAKRLGGSLSMGFLGDRIVVDQFPFHASTLGIGRLAERMASRGIEKVAIGEEVTLPEM
                     KRLVYFVAGVGEIDGSRPWKQISFGRIQGDEPAEFPASGAGEALPAPQVLAGATDVLK
                     DVLHSIAVKGPLGNVEEGRDIVAAVMKGLREEEFLIDRMIRLQDRDDYTVAHSLNVCV
                     MVVAQAARLDLSEAVIRDIGLAALLHDIGKELVPAEILNKPGKLDSGEFAK"
BASE COUNT          265 a          485 c          529 g          251 t
ORIGIN      
        1 gctggagggg gcgatcccgc ggatgatcga cgagagggac ttcgagttgc tggatcgcgt
       61 cctgtcgtcc ttccttcacg cctcggaatc ggggacgccg gcacgcaggg ccgcggcaaa
      121 cgacgccctg cgctccgtgg aattcctccg gatcgtcgac gcgatccttg cgagtccggg
      181 atccctcgag gaagaaaaag gcggggcggc gctcctctcg aaacacgggg ccctcgcggc
      241 ggaggccctt ctccagaaac ttcaggacga agaggagccg ggacggcgga aaatcctcct
      301 gtcgctggtc ctgcgccttg gggagaaggc cgtgccgtcg atcctctcgc gcatcgaagg
      361 gcaacgctgg ttcttccttc ggaacctctg tttcctcctc ggcgagatcg gggcccccgc
      421 tggcatcccg ccgctcgtgg gcatgctgtc cgccagggag ccgaaggtcc gcagggaggc
      481 ggtccaggcg ctcgggaaga tccggatgcc cgacccggac gccatcgccg tgctcggcag
      541 aacgctgctc cacgaaccgt tcttctcctc ctcacgcgaa gattccgtcc gcatcgacgc
      601 cgccatcgcc ctctcccgca tcgggggcac ggaggcaatc gccttccttc attacgggaa
      661 atcgtcgagg aagaaggcgg tgcgcgagca gtgcgaggcc ctgttgcgga cgaggggaac
      721 ggaatgaagg cgcaggaggt tttcctcgcg atcctcaccg accttggccg gtccatccag
      781 gcggcggccc tctacccgga gacgcaccaa cgcgtccagg aaccgctggc acgcctccac
      841 agaagggtct ggaacgaggc gaaacggctc gggggcagcc tgagcatggg gtttctggga
      901 gaccggatcg tggtcgacca gttccccttc cacgcctcta ccctggggat cgggcgtctc
      961 gccgaaagaa tggcgtcccg tggaatcgag aaggtcgcca tcggggaaga ggtcaccctt
     1021 ccggagatga agcggctcgt ttattttgtg gcgggcgtcg gggagatcga cgggtcccgg
     1081 ccgtggaaac agatctcctt cggcaggatc cagggggacg agccggcgga gttcccggcg
     1141 tctggcgccg gcgaggccct gcccgctccc caggtgctcg ccggggcgac ggatgtgctg
     1201 aaggacgtgc tccactccat cgccgtcaag ggccccctcg ggaacgtgga agagggaagg
     1261 gacatcgtgg ccgcggtgat gaagggattg agggaagagg aattcctcat cgaccggatg
     1321 atccgcctcc aggaccgcga cgattacacg gtcgcccact ccctcaacgt ctgcgtgatg
     1381 gtggtggccc aggcggcccg cctggatctc tccgaggcgg tgatccggga catcgggctg
     1441 gccgcgctcc tgcacgatat cgggaaggaa cttgttcccg cggagatcct gaacaagccg
     1501 gggaaactgg attccgggga gttcgcgaag
//
LOCUS       DASVJL010000300         7478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_798111, whole genome shotgun sequence.
ACCESSION   DASVJL010000300 DASVJL010000000
VERSION     DASVJL010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798111"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VF357_06355"
     CDS             <1..367
                     /locus_tag="VF357_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523804.1"
                     /translation="SDVVITTALIGGVKAPRLITAEMVRGMKQGSVIVDAAAEGGGNC
                     ALTRPGETIVEGGVTICGPINLPAEMPWHASTLYSRNLTAFVLAFWKDGRLNLDLGDE
                     ILRGALVTHGGEVRLATAG"
     gene            389..685
                     /locus_tag="VF357_06360"
     CDS             389..685
                     /locus_tag="VF357_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX7523805.1"
                     /translation="MSPSLEMGLYVFVLATFLGLEIIRRVSPLLHTPLMSLTNAISAI
                     TIVGSILVAGEQKTTLSTVLGTLAVTCSMINIVGGYLITYRMLRMFRKSGKDKP"
     gene            682..2058
                     /locus_tag="VF357_06365"
     CDS             682..2058
                     /locus_tag="VF357_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX7523806.1"
                     /translation="MIDRAIPLLYLTAAVSFILALKWLSAVPTARRGVIVGAAGMALA
                     VAGTLLKPEIESYRWIAVAFVAGTAIGIPMAMMPMTAVPQRTALSHAFGALAAALVGT
                     AEYYLRAPAIDRFTMGALVLEVLLGFLTFTASLMAFGKLQEILPTRPITWRNQNILNF
                     TLLGLAVASGAYLVARPDAIRLFPVFGGLALVFGVMLIIPIGGADMPTVISLLNSYAG
                     LSAAAMGFVLDNKLLIIAGALDGSSGLILSVIMCRAMNRSFTNVLFGAFGQVQATAGA
                     AGEQRAVRSATPEEAAGILQVASRVIVVPGYGMAVAQAQHKVRELYDVLTKAGVDVRF
                     AIHPVAGRMPGHMNVLLAEANIPYDRLVEMEDVNSDFAQADVALVIGANDVTNPAARH
                     DRNSPIYGMPILDVDKARTVMVVKRSMSPGFAGIDNELYYMDRTLMVFGDAKAVVGNL
                     VKEFAGGT"
     gene            2116..2598
                     /locus_tag="VF357_06370"
     CDS             2116..2598
                     /locus_tag="VF357_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HEX7523807.1"
                     /translation="MGPVDYIPRIRDNYARLGYKAYNWVVNQDTAPWAPLRKPLSQCR
                     LGLAASGGIYVTGQVAFHYKDDTSLREIPTDVDTKELRATHFAYDLSDARTDPNVVFP
                     IDTLRRLVRDGFLGGLASRFHTFMGGIYSSRRVTEDLAPRLTARFLEDDVDAVLLVPV
                     "
     gene            2668..3003
                     /locus_tag="VF357_06375"
     CDS             2668..3003
                     /locus_tag="VF357_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523808.1"
                     /translation="MTSARDITRAVNPPRAAFLDFPLGHTTGKPHEPDLQRRILVEAL
                     SSFETMTSPGSMKELPFRWSEDEEWKAKAFAGGDDRTPRHDTPQYQDAEDRRRAEEGG
                     SPSCPVCRS"
     gene            3005..4219
                     /gene="serA"
                     /locus_tag="VF357_06380"
     CDS             3005..4219
                     /gene="serA"
                     /locus_tag="VF357_06380"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:NF008759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX7523809.1"
                     /translation="MLAPENPMKILMLENIHPDAGAIFAAAGHAVEQVPHSPARKVLA
                     QGIGDISVLGIRSKTRVTADLLERAPELLAIGAFCIGTEQVDLEACSRRGVAVFNAPY
                     SNTRSVVELALGEMILLLRGVFDRSTNLHKGVWVKSSEGFHEIRGKKLGIVGYGNIGS
                     QLSILAEAMGMEVGYHDLVEKLSLGNARKCSLEELLRVSDIVTVHVDGREENRGLVGE
                     REFAAMKDGVIFLNLSRGFVVDVEALARNVRGGKVRGAAVDVFPEEPRTNKDPFSSPL
                     QGLPNVILTPHIGGSTEEAQQGIGGFVAARLLDYLSLGSTSTSVNFPDLSPSSTPQRH
                     RFAHIHENLPGMLAKINDVFARQGVNIVGQRLETRGRLGYALIDVALGCDLGIVEELR
                     RIPNTVRTRLVY"
     gene            4261..6273
                     /locus_tag="VF357_06385"
     CDS             4261..6273
                     /locus_tag="VF357_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX7523810.1"
                     /translation="MTSPLSPLFRPSRIAVIGASSNPDKMGFQIFRNIKEAGFAGEIV
                     PVNPKGEVILGVPSIKSAAELPEGTDLAVVIIPAKLVPGTMRELGGRKVKSAIVITGG
                     FAESGEPGAALQAEMAQAAQAAGIRVVGPNCQGVNYPYHGVCASWPLITRRGEIAIVS
                     QSGTVGAALIDWASEERLGFSAFVSMGNRSDVDEADLIEFFAEDPHTKVIALYIEGVK
                     DAPKFLSAVRACPKPVVIFKAGRTERGRKAAESHTRSLAGRDEIYDAVFRQNGVHRAA
                     TLEELYDFAKALAYVPPPKGPRMLIVTSSGGSAIIATDVAEEEGLRVTPLPTELSRKL
                     REILPSHCIVGNPLDLTGDTDAARYRNVLDAAEGEFDVVMTIFGDPIPGASEVIRPGR
                     CELVSYLGGADVERAERLRFHEKKIAVFPTPERAVKALSCHVRFHRDRFPLSVEGTGH
                     GEAVSGTAMTPANSMAFLARSGFPLTRFRRSGTEDEAVAAARGIGLPVAVKMNSPDVT
                     HKSDAGGVFLDVADDGGVRAAFRAIRATEERLGARAGGALVCAMAPTGHEVIVGVTKD
                     PQFGHAVMFGLGGIFVEVMKDVSFRVAPLSEKDAVEMIREIRGYRALTGLRGKPAGDL
                     DALRMLLLQLSAFLASHPEIEEMDLNPVIVHARGLTIADARVVVRR"
     gene            complement(6278..>7478)
                     /locus_tag="VF357_06390"
     CDS             complement(6278..>7478)
                     /locus_tag="VF357_06390"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7523811.1"
                     /translation="ASGKAGTVFSGYFRDALLPRVDRMRPRGVAISVNYRHQVLPAFE
                     LAGLLRRRMPGVPIYGGGGMFTSWRGSLRGMGLAVLPFDRVGFGPGERALAAVAEGAS
                     TGVGPFFEDGDAVEFLPDFGVGPLREYLSPEPVLPVAATRGCYWRRCRFCPEAVAPIH
                     PYRATDARRFPTLLRELSDRYGVRRFHLTDNAIPVDILRSMADGGGVLRGLSWHGFAR
                     FERELIEPEFASSLAAAGCTMLQLGLESGSQRLLDRMGKGTRVADASAILANLSRAGI
                     VAFVYVMFGAPGETETDAERTRSFLAEHAGEIGFLNLSILNLPREASWPGIAEGSAQG
                     EDGFPDEVEPLGLYRKMPASDGWGRAQARRFLQKRILGDPAIRAIVARTPPWFGDSHA
                     FFFRGTT"
BASE COUNT         1237 a         2536 c         2551 g         1154 t
ORIGIN      
        1 gtcggacgtg gtcatcacga cggcgctgat cggcggcgtg aaggcaccga ggctcatcac
       61 cgccgagatg gtgcgcggga tgaagcaggg ctcggtgatc gtggacgcgg ccgccgaagg
      121 gggaggcaac tgcgcactga cccgtccggg ggagacgatc gtcgagggcg gcgtgacgat
      181 ctgcggtccc atcaacctcc ccgccgaaat gccctggcac gcaagcacgc tttactcgcg
      241 caacctgacg gcattcgtcc ttgccttctg gaaggacggc cgtctcaatc tcgacctggg
      301 cgacgagatc ctgcggggcg ccctcgtgac ccacggaggc gaggtccggc tcgccacggc
      361 gggctgacgg gcataaggag gccttcccat gagtccgtcc ctcgaaatgg gactctacgt
      421 cttcgtactg gccacgttcc tcgggctgga gatcatccgc agggtgtcgc cgcttcttca
      481 cacccccctg atgtccctga cgaacgccat ctcggccatc acgatcgtcg gctccatcct
      541 ggtggccgga gagcagaaga ccaccctctc caccgtcctc ggtaccctcg cggtgacctg
      601 ctcgatgatc aacatcgtcg gcgggtacct gatcacctac cgcatgcttc ggatgttcag
      661 gaagagcgga aaagacaagc catgatcgac cgggccattc ccctgctcta cctcacggcc
      721 gcggtctcct tcatcctggc cctcaagtgg ctctccgcgg tcccgacggc gcgccggggc
      781 gtgatcgtgg gggcggccgg gatggcgctg gcggtcgcgg ggacgctgct gaagccggag
      841 atcgaaagct atcggtggat cgcggtcgcc ttcgtggccg ggaccgccat cggcatcccg
      901 atggcgatga tgccgatgac cgcggtgccg cagcggaccg ccctctccca cgccttcggt
      961 gcgttggcgg cggcgctggt ggggacggcc gaatactacc tgcgggcgcc cgccatcgac
     1021 cgcttcacca tgggggcgct ggtcctcgaa gtgttgttgg ggtttctcac cttcaccgcc
     1081 tcgctgatgg cgttcggaaa gctccaggag atcctcccca cccgccccat cacgtggcgg
     1141 aatcagaaca tcctcaattt cacgctcctc gggctcgcgg tggcatcggg ggcatacctc
     1201 gtcgcccgcc cggacgcgat ccggctgttc ccggtttttg ggggcctcgc gctcgtgttc
     1261 ggcgtgatgc tcatcatccc catcggcggc gcggacatgc cgaccgtgat ctctcttctc
     1321 aactcctacg cgggcctgtc cgcggcggcc atgggattcg tgctcgacaa caagctgctg
     1381 atcatcgccg gcgcgctcga cggctcgtcc ggcctcatcc tgtcggtgat catgtgccgc
     1441 gcaatgaacc ggtcgttcac caacgtcctg ttcggcgcgt tcgggcaagt ccaggcgacg
     1501 gccggggccg cgggggaaca acgcgcggtg cgaagcgcca cgccggaaga ggccgcggga
     1561 attctgcagg tggcgagcag ggtgatcgtc gtccccggat acgggatggc ggtggcccag
     1621 gcgcagcaca aggtccggga gctgtacgac gtcctgacga aagcgggcgt ggacgtccgg
     1681 ttcgccatcc acccggtggc ggggcgcatg ccggggcaca tgaacgtgct cctcgccgag
     1741 gcgaacatcc cgtacgaccg gctggtggag atggaggatg tgaactccga cttcgcgcag
     1801 gccgacgtgg cgctggtcat cggcgccaac gacgtgacga accccgcggc gcggcacgac
     1861 cggaacagcc cgatctacgg aatgccgatc ctcgacgtcg acaaggcacg gacggtgatg
     1921 gtcgtcaagc ggagcatgag ccccggcttc gcggggatcg acaacgagct gtattacatg
     1981 gaccgcacgc tcatggtctt cggggacgcc aaggcggtcg tcgggaatct cgtgaaggag
     2041 ttcgccggag gaacctgatc cggggtggca ccgaacggta ccggcttggc gtactatgga
     2101 aacggggagg aaccgatggg acccgtcgac tacattccga gaatccgcga caattacgcg
     2161 cggctggggt acaaggcata caactgggtg gtcaaccagg acaccgcgcc gtgggctccg
     2221 ctgcgcaagc ctctttcgca atgccggctg ggattggccg cctcgggggg gatctacgtc
     2281 accgggcagg tggcgttcca ctacaaggac gacacctcgt tgcgcgaaat cccgaccgac
     2341 gtcgacacga aggagctgcg ggcaacccac ttcgcctacg acctgtccga cgcgcgcacg
     2401 gacccgaacg tcgtcttccc gatcgacacg ctgcgccgac ttgtgcggga cgggttcctc
     2461 ggggggctgg cgagccgctt ccacacgttc atgggaggga tctactcctc ccgccgcgtt
     2521 accgaggatc tggccccgcg tctgaccgcc cggttcctcg aggacgacgt cgacgcggtg
     2581 ctgctcgttc cggtctgacc cgtctgccac cagtccgtgg gactggtcgc aaggcacctg
     2641 gagaccgccg ggatcccgac gctctgcatg acaagcgcgc gggacatcac gcgggcggtc
     2701 aaccctcccc gggcggcgtt cctcgacttc cccctcgggc acacgaccgg caagccgcac
     2761 gagcccgatc tgcagcgccg gatcctcgtc gaagcgctct cgtccttcga gacgatgacg
     2821 tctccgggct ccatgaagga gcttccgttc cgatggtcgg aggacgagga gtggaaagcg
     2881 aaggcattcg cgggagggga cgaccggacg ccccgccacg acaccccgca gtaccaggac
     2941 gcggaggacc gccgccgggc ggaagagggg ggttccccct cctgccccgt ctgccgatcc
     3001 tgacattctc gccccggaga acccgatgaa gatcctgatg ctggagaaca tccaccccga
     3061 cgcgggggcg atcttcgcgg cggccggtca tgccgtggag caggtgccgc acagccctgc
     3121 ccggaaggtg ctcgctcaag ggatcgggga catctccgtt ctcggcatcc gctcgaaaac
     3181 ccgtgtgacc gccgaccttc tggaacgcgc cccggagctt ctggccatcg gcgcgttctg
     3241 catcgggacc gaacaggtcg acctcgaggc gtgcagccgg cgcggggtag cggtcttcaa
     3301 cgccccctac agcaataccc gcagcgtggt cgaacttgcc ctgggcgaga tgatcctgct
     3361 cctgcgcggc gtgttcgacc gcagcacgaa cctccacaaa ggggtctggg ttaagtcctc
     3421 ggaggggttc cacgagatcc gcgggaagaa gctcgggatc gtcgggtacg ggaacatcgg
     3481 ctcccaactc tccatcctcg cggaagcgat ggggatggag gtgggctatc acgatctcgt
     3541 ggagaagctt tcgctgggga acgcccggaa gtgctccctg gaggaacttc tgcgggtctc
     3601 ggacatcgtc accgtccacg tcgacggacg ggaagaaaac cggggcctcg tcggggagag
     3661 ggaattcgcg gcgatgaagg acggggtgat cttcctcaac ctgagccgcg gcttcgtggt
     3721 ggacgtggag gcgctcgcgc ggaacgtacg cggcgggaag gtccgcggcg cggcggtgga
     3781 cgttttcccg gaggagcccc ggacgaacaa ggaccccttc tcctcgccgc tgcaggggct
     3841 cccgaacgtg atcctgaccc ctcacatcgg cggctcgacc gaggaggcgc agcaggggat
     3901 cggaggattc gtggcggcgc ggcttctcga ctacctgtcc ttggggagca cgtccacgag
     3961 cgtgaacttt ccggaccttt ctccgtcctc gactccccag cgccaccgat tcgcccacat
     4021 ccatgaaaat ctccccggga tgctggcgaa gatcaacgac gtcttcgcgc ggcaaggcgt
     4081 caacatcgtc gggcagcgac ttgagacgcg cggccgcctg gggtacgcgc tgatcgacgt
     4141 tgcgctgggg tgcgacctcg ggatcgtgga ggaactccgg cggatcccca acacggtccg
     4201 cacccgcctc gtctactgag ggaccgtgac ggaaaccccc tggaagcaag gagcgcgcgg
     4261 atgacctctc ccctgtctcc cctgttccgt ccctcccgga tcgccgtgat cggcgcatcc
     4321 tcgaaccccg acaagatggg gttccagatc ttccgcaaca tcaaggaggc cggcttcgcc
     4381 ggcgagatcg tccccgtaaa cccgaagggg gaggtgatcc tgggcgtccc ctccataaaa
     4441 tccgccgccg aactccccga ggggaccgac ctcgcggtcg tgatcatccc ggcgaagctc
     4501 gtccccggga cgatgcggga gctgggggga cggaaggtga aatccgccat cgtgatcacg
     4561 ggggggttcg ccgagtccgg cgagccgggg gcggcgctcc aggcggagat ggcgcaggcg
     4621 gcgcaggcgg cgggcatccg cgtggtcggg cccaactgcc agggcgtgaa ctacccgtac
     4681 cacggcgtgt gcgcctcgtg gccgctgatc acccggcggg gggagatcgc gatcgtctcg
     4741 caaagcggta ccgtcggcgc ggcgctgatc gactgggcgt cggaggagcg gctcgggttc
     4801 tccgccttcg tcagcatggg gaaccgttcg gacgtcgacg aggcggacct gatcgaattc
     4861 ttcgcggagg acccgcacac gaaggttatc gccctctaca tagaaggggt gaaggacgcc
     4921 ccgaagttcc tctccgccgt ccgcgcctgc ccgaagccgg tcgtgatctt caaggccggg
     4981 cggaccgagc ggggacgcaa ggcggcggag tcgcacaccc ggtcgctcgc ggggcgcgac
     5041 gagatctacg atgccgtgtt ccgccagaac ggcgtgcacc gcgccgccac gctcgaggag
     5101 ctgtacgact tcgcgaaggc cctcgcctac gtcccgcccc ccaagggccc gcgaatgctg
     5161 atcgtcacga gctccggcgg gtccgcgatc atcgccaccg acgtcgccga ggaggaaggg
     5221 ctgcgcgtca ccccgcttcc caccgaactc tcccggaagc tgcgggagat cctcccctcc
     5281 cactgcatcg tcggcaaccc cctcgatctc accggggaca ccgacgccgc acgctaccgg
     5341 aacgtgctcg acgcggccga gggggagttc gacgtggtga tgacgatctt cggggacccg
     5401 atccccgggg cctccgaagt gatccgcccc ggccggtgcg agctggtgtc ctacctcggc
     5461 ggcgccgacg tggagcgcgc ggagcggctg cggttccacg agaagaagat cgccgtcttc
     5521 cccacgccgg agcgggccgt gaaagccctc tcctgccacg tccggttcca ccgtgaccgc
     5581 ttcccgctct ccgtggaagg gacgggacac ggggaggcgg tgtcgggtac ggcgatgact
     5641 cccgccaact cgatggcgtt cctcgcccgc tccgggttcc cgttgacccg cttcaggcgc
     5701 tcggggaccg aggacgaggc ggtcgccgcg gcgcggggga tcggccttcc cgtcgcggtg
     5761 aagatgaact ctcccgacgt cacgcacaag agcgacgccg ggggggtctt cctggacgtg
     5821 gccgacgacg gcggcgttcg ggcggcgttc cgggcgatcc gggccacgga agaaaggctt
     5881 ggcgcgcggg ccggcggggc gctcgtctgc gcgatggcgc ccacagggca cgaggtgatc
     5941 gtcggcgtga cgaaggatcc gcagttcggc catgccgtga tgttcggcct gggggggatc
     6001 ttcgtcgagg tgatgaagga cgtctcgttc cgcgtggcgc cgctctcgga gaaggacgcc
     6061 gtggaaatga ttcgggagat ccgggggtac agggcgctca cggggctgcg cgggaagccc
     6121 gccggggacc tcgacgccct gcggatgctc ctgctgcaac tctcggcgtt cctcgcgtcg
     6181 cacccggaga tcgaggagat ggacctcaac ccggtcatcg tccacgcccg ggggctgacg
     6241 atcgcggacg cccgggtggt cgtgcggaga taagcgctca ggtcgttccg cggaagaaga
     6301 aggcgtggct gtcgccgaac cagggcgggg ttcgggccac gatggcgcgg atcgccgggt
     6361 cgcccaggat ccgcttttgc aggaaccgtc gggcctgcgc cctcccccag ccgtcgctcg
     6421 cgggcatctt ccgatacagg ccgagcggct cgacctcgtc ggggaacccg tcttccccct
     6481 gtgcactccc ctccgcgatc ccaggccaag acgcctcgcg gggcaggttg aggatcgaca
     6541 ggttgaggaa cccgatctca cccgcatgtt ccgcgaggaa cgatcgcgtc cgctccgcgt
     6601 cggtctccgt ctctcccggg gcgccgaaca tcacgtacac gaaggcgacg atccccgctc
     6661 gagaaaggtt cgccaggatc gccgaagcgt cggcgacgcg cgtccccttc cccatccggt
     6721 cgaggagtcg ttgggacccg ctctccagcc caagctgaag catcgtgcaa cccgccgcgg
     6781 ccagcgacga ggcgaactcc ggctcgatca gttcccgctc gaagcgggcg aacccatgcc
     6841 acgaaaggcc gcgcagcacg ccccccccgt ccgccatcga ccggagaatg tcgaccggga
     6901 tcgcgttgtc cgtcaggtga aaccgccgga cgccgtaccg atcggacagc tcccgcagga
     6961 gcgtggggaa cctccgcgcg tccgtcgcac ggtacggatg gatgggggcg accgcctcgg
     7021 ggcagaaccg gcatctcctc cagtagcatc ccctcgtcgc ggcaacgggt aggaccggct
     7081 ccggggacag atactcccga agcggcccga ccccgaaatc ggggaggaac tccaccgcgt
     7141 ccccgtcctc gaaaaagggg ccgacgcctg ttgaagcacc ctcggccacc gcggccaggg
     7201 cgcgctcccc gggaccgaac ccgacccggt cgaaggggag gaccgcgagc cccatgccgc
     7261 gaagggaacc cctccatgag gtgaacatcc ccccaccgcc gtagatgggg acgccgggca
     7321 tgcggcgtcg cagcaatccc gcaagctcaa aggcggggag gacctggtgc cggtagttca
     7381 cggagatcgc gacaccgcgc ggacgcatcc ggtcgacgcg agggaggaga gcgtcccgga
     7441 agtagccgga gaagaccgtt cccgctttcc cggacgcc
//
LOCUS       DASVJL010000301         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_610108, whole genome shotgun sequence.
ACCESSION   DASVJL010000301 DASVJL010000000
VERSION     DASVJL010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610108"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VF357_06395"
     CDS             <1..480
                     /locus_tag="VF357_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7523812.1"
                     /translation="IVVFDKEKCISCKYCVSACPFNIPRYGADDKVTKCHLCFDRVGA
                     GRVPACAQACPTQTLQFGSRDALIAKAKAAGKKLYGESALSGLGVVYALEDKPEAYGL
                     PADPSIPISIFLWKDVVRPLGILGFWGSVAAVVVHYVTFGSRQLEEDGKGKGGDANG"
     gene            473..1087
                     /locus_tag="VF357_06400"
     CDS             473..1087
                     /locus_tag="VF357_06400"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX7523813.1"
                     /translation="MGKKYVERFNTAERVLHWFVSVSFFTLLLSGLGLYSRLFHGYFD
                     LFGGGEGAILAHKYAGVVFFISSLMLFFNHAKEMFTFDEDDRKWCRSLGGYLSKNPEH
                     INSGKFNAGQKLFGLFMGIATLLLGITGVINWIPRSFPREIVQLSLLVHGLLFVAFVM
                     LMVVHVYLTTIGNPGTLDGMLYGNVRRIWARTHHPKWYKEVGGE"
     gene            1103..1945
                     /locus_tag="VF357_06405"
     CDS             1103..1945
                     /locus_tag="VF357_06405"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEX7523814.1"
                     /translation="MSLTADKIAHLRKTALGHPEYLDVLRPFEEIFAYVEGKEDGTGI
                     RFIVPEGNGAGRVQGGLPLLSPEALSVDRDAATAFLSGLLDVLRRVGREGHADLDRIG
                     GGLSDGSLDLASLYVACLTRKRDVVDQAAASHSVQAPLLAFVLEIPLKTALERVSSSL
                     PPERFDGWKEGYCPVCGSRAGMAELSGDEGKRRLSCSACFFRWPFPRIQCPYCGNADP
                     ETLSYFTAGDGPTRVGVCRKCSRYLKTRDARLGNADVPLEAEDLATLHLDLLAGKEGF
                     ERGK"
BASE COUNT          323 a          650 c          713 g          374 t
ORIGIN      
        1 atcgtcgtct tcgacaagga gaagtgcatt tcgtgcaagt attgcgtctc cgcctgcccg
       61 ttcaacatcc cccggtacgg ggcggacgac aaggtgacca agtgccacct ctgcttcgac
      121 cgggtcgggg cgggcagggt cccggcatgc gcccaggcgt gtccgaccca gacgctgcag
      181 ttcgggagcc gggacgcctt gatcgcgaag gcgaaggcgg cggggaagaa gctgtacggg
      241 gaaagcgccc tctcggggct gggcgtcgtc tacgcgctcg aggacaagcc cgaggcgtat
      301 ggccttcccg cggacccgtc gatcccgata tcgatcttcc tgtggaagga cgtggtgagg
      361 ccgctcggaa tcctcggctt ctggggaagc gtcgccgcgg tggtggtcca ctatgtgacc
      421 ttcggatcgc gccagctcga ggaggacggg aaggggaagg gaggggacgc gaatgggtaa
      481 gaaatacgtc gagcggttca acacggccga gcgggtgctc cactggttcg tctccgtgtc
      541 gttcttcacc ctgctcctgt cggggctggg gctctactcc cggctcttcc acggctactt
      601 cgacctcttc ggcggcgggg agggggcgat cctcgcccac aagtacgcgg gggtggtctt
      661 cttcatcagt tccctgatgc tcttcttcaa tcatgcgaag gagatgttca ccttcgacga
      721 ggacgaccgg aaatggtgcc gctcgctggg ggggtacctt tcgaagaatc cggagcacat
      781 caacagcgga aaattcaacg ccgggcagaa gctgttcggc ctcttcatgg ggatcgcgac
      841 gctgctgctc gggatcacag gggtgatcaa ctggatcccc cggtcgtttc cgcgggagat
      901 cgtccagctc tccctgctgg tccacggcct cctgttcgtg gcgttcgtga tgctgatggt
      961 ggtgcacgtc tacctcacca cgatcggcaa ccctgggacg ctcgatggga tgctttacgg
     1021 caacgtccgc cggatctggg cgcggacgca ccacccgaaa tggtataagg aggtgggggg
     1081 cgaataatcg acggggggcg ggatgagtct tacggcggac aagatcgcgc acctccggaa
     1141 gaccgcgctc gggcatccgg agtacctcga tgtgctccgc ccgttcgagg agatcttcgc
     1201 atacgtcgag gggaaggagg acgggacggg gatccgcttc atcgtcccgg aggggaacgg
     1261 ggccgggcgg gtgcagggag gtcttccgct cctctccccc gaggcgctct ccgtggatcg
     1321 ggacgccgcg acggccttcc tgtccgggct gctcgacgtc ctgcggcgcg tcggcaggga
     1381 ggggcacgcg gatctcgatc ggatcggggg ggggctttcc gacggttccc ttgaccttgc
     1441 ctccctgtac gtcgcctgtc tgacccggaa gcgggacgtc gtggaccagg cggcggcgtc
     1501 ccattcggtc caggcgccgt tgctcgcctt cgtcctcgag atcccgttga agaccgccct
     1561 tgagcgcgtt tcctcctccc ttcccccgga acggttcgac ggctggaagg aagggtattg
     1621 tcccgtgtgc ggctcccgcg ccgggatggc cgaactctcc ggggacgagg ggaagcggcg
     1681 gctctcctgc tccgcctgtt tcttccggtg gccgttccca cggatccaat gcccctactg
     1741 cggaaacgcc gacccggaaa cgctctccta tttcaccgcg ggggacggtc ccacccgggt
     1801 tggtgtgtgc cgcaaatgca gccggtacct gaagacgcgc gacgcgcgcc tcgggaacgc
     1861 ggacgtgccg ctggaagccg aagacctcgc caccctccac ctggaccttc tggccgggaa
     1921 ggaagggttc gagaggggga aatgagccgc ctccccggag acgacgcgaa gaggggagac
     1981 gcccctcccg tcctgcggta cgacgggcgc cgattggctc ccgtccggca tctccccgtg
     2041 cgggaggtcc ccgtcgccct
//
LOCUS       DASVJL010000302         4187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_235541, whole genome shotgun sequence.
ACCESSION   DASVJL010000302 DASVJL010000000
VERSION     DASVJL010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235541"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VF357_06410"
     CDS             <1..815
                     /locus_tag="VF357_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX7523815.1"
                     /translation="AHSIVQKTAKVTITWTEWCVGFLPVGLLLFLAVPYLAYKIYPPT
                     PKVSEDVTRWAAAELTKMGPFTRKEGMMAGLAVLALLLWIFGGSLLNATTVALLVLSL
                     MIVTGIVTWDDVLGNKQAWNVLVWFGTLVTLAEGLNRVGFLKWISEAAAKSMTGMPIP
                     MMMVLLIVFFFVAHYMFASITAHVTGLLPVVLATAMAVPGVPVKMFAMLLCYSLGIMG
                     VITPYATGPSPIYYGSGYISRGAYWTLGLVFGAIFLAALLLVGIPYLRFLSP"
     gene            complement(1003..2733)
                     /locus_tag="VF357_06415"
     CDS             complement(1003..2733)
                     /locus_tag="VF357_06415"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7523816.1"
                     /translation="MNTPGSFDSLLVESVTDGMVAISPEGRILYANRSFQERSGHRLE
                     DIVGKHCREVVLESICDSGCPFAEVLDTGKAADRFDVEVRGRDGEAMSACINFTPLRD
                     ASGGIIGVVEVIRDITKLKELKDTLKRTDSMYRRERVKIKTILDNIPSGVYTVDQEMN
                     LLSFNPSLERITGFRSDEVLGKKCMEVLRSDFCEAGCPLRRSMRTGETLRGIEVNLRA
                     KDGTVVPIASSTAVLRDEDGRPIGGICSFRDLRELHSVPFPHGDVREFQGMLSKNPQI
                     HEIFDLIETVAESDAYVLIEGESGTGKELVARAIHRLSARAEKPFLGVNCASLNENLM
                     ESELFGHVRGAFTGAVKDRAGRFEMAEGGTLFLDEVSEIGPHLQAKLLRVIQEREYQR
                     VGETGARKTNVRIISATNKRLKELLAGGAFRDDLYYRLNVVTITSPPLRERKEDIPLL
                     VEHFLRRPPDRENVKKRRFSPLAMQAFLEYPWPGNVRELGNAVERAKICSRGEIIEES
                     ALPAEIRRRGSGVPRGAGSFGDKGWGLEADRVKETLARCRGNREEAAEQLGISRVTLW
                     RRMKRLGVSA"
     gene            3060..>4187
                     /locus_tag="VF357_06420"
     CDS             3060..>4187
                     /locus_tag="VF357_06420"
                     /inference="COORDINATES: protein motif:HMM:NF023281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3373 family protein"
                     /protein_id="HEX7523817.1"
                     /translation="MNRKGAVLFAAILVAALALPQGILAADEDIQKKVDALSKEVQAL
                     QQQVAQSKDGKKSSSDWLTIGGDYRFRVDSLSGKVPNFLSFADAIAWQLGGMVGSPAI
                     TPGTTVKNETLYTNRFGLNIKAKVMQNVTFNSRLVMYKEWGSQANDASRGDFFADRIG
                     AFDGTLGHVPSDGEVAVDQVYMTVNNIFGQPIWFSIGRRPSTGGIPTHLRQDNEKPGV
                     SGVSGLLVDYAYDGLTLGYAPDIDALPGAYAKFCYGRGFQRGFHSGQPGDVKNMDMMG
                     IVVVPYDTDPLTVHLQVNHAINIIDFPVIKDSTLGSLRPTTNLGNIDQFGVVVMSTLK
                     KVGPGTLNVFASGALDKTHPNENHPTFGGMVLSNIGLMYTGT"
BASE COUNT          748 a         1354 c         1262 g          823 t
ORIGIN      
        1 tggcccactc catcgtccag aagacggcga aggtgaccat cacctggacc gaatggtgcg
       61 tgggatttct tcccgtggga ttgctgctct tcctggccgt gccgtacctg gcctataaaa
      121 tctatccgcc cacgccaaaa gtgagcgagg acgtcacccg ctgggcggcc gcggagctga
      181 ccaagatggg cccgttcacc cggaaggaag ggatgatggc cggcttggcc gtcctggcac
      241 tcctcctgtg gattttcggc ggatccctgc tgaacgcgac gacggtcgca ctccttgtgc
      301 tgtccctcat gatcgtgacg ggcatcgtca catgggacga cgtattgggg aacaagcagg
      361 cgtggaacgt gctcgtgtgg ttcgggactc tggtgacgct cgccgaaggc ctcaacaggg
      421 tcggattcct gaaatggatc tccgaagcgg cggcgaagtc gatgaccggg atgccgatcc
      481 cgatgatgat ggtcctgctg atcgtgttct tcttcgtggc gcactacatg ttcgccagca
      541 ttaccgccca cgtcacgggg ctgttgccgg tcgtcctggc caccgccatg gcggtccccg
      601 gcgtcccggt caagatgttc gcgatgctcc tttgctacag tctggggatc atgggagtga
      661 tcacgccgta cgcgaccggt ccgtctccca tctactacgg gagtggatac atcagccgcg
      721 gggcgtactg gacgctggga ctggttttcg gggccatttt cctggccgcg cttcttctgg
      781 tcggtatccc gtacctgcgt ttcctgagtc cgtaatggcg tgaggatgaa cgcatgaggg
      841 agtcgcttgc cgggggtgcg gcgaaggtgg aagggttcat cggtggaagg gtcatgacgc
      901 acccccggca aaccgccgaa ctgtatacag tatacaattc ggccacggga tgtcaacggg
      961 ggttcccggc gatgggcgat ctccgccgcc gggaccgggg atctacgccg aaacgcccaa
     1021 ccgtttcatc cggcgccaca gggtgacacg gctgatgccg agttgctccg ccgcttcttc
     1081 gcggttcccg cggcagcggg cgagcgtttc cttgactcgg tctgcctcga gtccccatcc
     1141 tttgtcaccg aacgatccgg cgccgcgggg aacccccgag ccccggcgtc ggatctccgc
     1201 cggaagggcg gactcctcga tgatctcccc ccgggagcag atcttcgccc gttccacggc
     1261 attccccaac tcccgcacgt ttcccggcca cgggtattcg aggaatgcct gcatggccag
     1321 gggggaaaat cttcttttct tcacattctc gcgatccggg gggcggcgga ggaagtgctc
     1381 cacgaggagg gggatgtctt ccttccgttc gcggagcggc ggagaggtga tggtgacgac
     1441 gttcaggcgg taatacaggt cgtcccggaa cgcccctccc gcgagcaact ccttcagccg
     1501 cttgttggtg gcggagatga tccggacgtt ggttttcctg gcgccggtct ctccaacccg
     1561 ctggtattcc cgttcctgga tcacgcgcaa cagtttcgcc tgcaggtgcg ggccgatctc
     1621 ggagacctcg tcgaggaaca gcgtcccgcc ctccgccatc tcgaaccgcc ctgcccggtc
     1681 tttgacggct cccgtgaacg ccccccggac gtgaccgaac agctcgctct ccatgaggtt
     1741 ctcgttcagc gaggcgcagt tcaccccgag gaacggcttc tccgcccggg cactcagtcg
     1801 gtgaatcgcc ctcgccacga gctcttttcc ggtgccgctt tccccctcga tcaacacgta
     1861 ggcgtcggac tcggcgaccg tctcgatgag gtcgaagatc tcgtggatct gggggttctt
     1921 gctcagcatc ccctggaatt cgcgcacatc tccatgcggg aagggaaccg aatgcaactc
     1981 ccggagatcc cggaaggagc agatcccgcc gatgggtctc ccatcctcgt ccctcaagac
     2041 cgccgtgctc gacgcgatcg gcaccaccgt gccgtccttc gcccgcaggt tcacctcgat
     2101 cccccgcaat gtttctccgg tccgcatcga ccgtctcagc gggcatccgg cctcgcagaa
     2161 gtcggaccgg aggacctcca tgcatttctt cccgaggact tcatccgaac ggaagccggt
     2221 gatccgttcg agcgacggat tgaaggagag gagattcatc tcctggtcca cggtgtaaac
     2281 gccggaaggg atgttgtcga ggatcgtctt gatcttgacc cgctccctcc ggtacatgga
     2341 gtcggtcctc ttgagggtgt ccttcaactc tttgagcttg gtgatgtccc ggatcacctc
     2401 gacgacgccg atgatgccgc ccgaggcgtc ccgcaacggc gtgaaattga tgcaggcgct
     2461 catcgcctcc ccgtcccttc cacgcacctc cacgtcgaac cggtccgccg cctttcccgt
     2521 atccaggacc tccgcgaagg ggcagcccga gtcgcagatc gattccagga cgacttcccg
     2581 gcaatgcttc ccgacgatat cctcgagacg atgccccgag cgctcctgga acgatcggtt
     2641 ggcgtacagg atcctccctt cgggggagat ggccaccatc ccgtcggtga cggattccac
     2701 gagcagcgaa tcgaacgatc ccggggtgtt catagcgtcc tccgtgcatg gggggtcgat
     2761 agtttcattg tatacagtat actaccggga cgcgggggat gtttcaaggg cttaacaatc
     2821 gttacgggat gaaacgcccg gcgctttcca tgcaaccatt tgaaatgtag agaatttctt
     2881 ggtttcaggc gatccgcctg aaacgactgt aacatcccgg ccgctcgccg gccttccctc
     2941 tgccgcaata aatcttatat tttccggtca gttaagggtt tctcgccggc tggcacgctc
     3001 ttcgcgtaac agaaaggtgg gacggtctac cctatccatg ttccgaaagg agaaggttca
     3061 tgaacagaaa aggagcggtt cttttcgcgg caatcctggt ggcggccttg gcgcttccgc
     3121 aagggatcct cgcggccgac gaggacatcc agaagaaggt ggacgccctg tcgaaggagg
     3181 tgcaggccct ccagcagcag gtggcccagt ccaaggacgg aaagaagtcc agctccgact
     3241 ggctgacgat cgggggcgac taccggttcc gcgtggactc cctgtccggg aaggttccga
     3301 actttctgag cttcgccgac gccattgcct ggcaattggg cggcatggtg ggcagcccgg
     3361 cgatcacccc cggcaccacg gtgaagaacg aaacgctcta caccaaccgc ttcggcctga
     3421 acatcaaggc gaaggtcatg cagaacgtca ccttcaacag ccggctggtg atgtacaagg
     3481 agtggggctc ccaggcgaac gacgcgtcgc ggggcgattt cttcgcggac cgcatcggcg
     3541 cgttcgacgg cacgctgggg cacgtcccgt ccgacgggga ggtcgccgtc gaccaggtgt
     3601 acatgacggt caacaacatc ttcggccagc cgatctggtt ctccatcggc cgccgccctt
     3661 ccaccggcgg aattcccacc cacctccggc aggacaacga gaagcccggc gtgagcggcg
     3721 tttcgggcct cctcgtcgac tacgcctacg acggcctgac gctcggctac gcacccgaca
     3781 tcgacgcgct gcccggcgcc tacgcgaagt tctgctacgg ccgcggcttc cagcgcggct
     3841 tccactccgg tcagcccggc gatgtgaaaa acatggacat gatgggcatc gtcgtggtcc
     3901 cgtacgacac cgatcccctc accgtccacc tccaggtcaa ccatgcgata aatatcatcg
     3961 acttcccggt catcaaggat tcgaccctgg ggagcctgcg cccgaccacg aatcttggaa
     4021 acatcgacca gttcggcgtc gtcgtgatga gcacgctgaa gaaagtgggc ccgggcaccc
     4081 tcaacgtgtt cgcctccggc gccctggaca agacccaccc gaacgagaac caccctacgt
     4141 tcggcggcat ggtgctgtcc aacatcggac tgatgtacac cggcacg
//
LOCUS       DASVJL010000303         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_798485, whole genome shotgun sequence.
ACCESSION   DASVJL010000303 DASVJL010000000
VERSION     DASVJL010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798485"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VF357_06425"
     CDS             complement(<1..482)
                     /locus_tag="VF357_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7523818.1"
                     /translation="MMQGQAALSKDRIRKASPRKSRKPDRDAIVNEFLPSIRIHAARL
                     KMRIPPHIETDDLVSSGVVGLLDALNRYDDSRGIKFKTYAEFRIRGAMLDYLREMDWF
                     PRSARQHSTRLQQTYTRLENILGRAPEEEEVAENLGVTVDQLRKQLATFMGMTVFSLD
                     A"
     gene            complement(648..1985)
                     /locus_tag="VF357_06430"
     CDS             complement(648..1985)
                     /locus_tag="VF357_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7523819.1"
                     /translation="MTSLRSRIAEVVDGIGLPADQREEIRRRLKTCLDEAERAGATER
                     DERIEALSTRVRQTEEQLAQAQKMEAIGRLTGGIAHDFNNLLTGILGYSSLLKTFLPE
                     NGRGYEAAAYIERSARRASELTRQLLAYSRRETPRFRPVDLRKVTGEAIGILARSVNK
                     NVEIHTDFHHPQETVTGDAGTLVQALLNLGVNASDAMPGGGHLTFSTSTFVSDGEVYL
                     NDVLVPEGRYVSICVADTGSGIPEEIRDEVFAPFFTTKAPGEGTGLGLSMVYSCVRTH
                     GGFVRLVSSVGVGTTFQILLPVMEESEDADEPRESETEIPRGTETVLVVDDEEIPLNL
                     LCDVLRSLGYTTLPAVSGEEGIEILRYAPEKVDLVIVDRIMAGMNGVETLARLRAIRP
                     TLRTILCSGTSEAEGKPSGAVPDGFDDFLQKPYERETLARTVRSVLDAKPSRF"
     gene            complement(1982..>2732)
                     /locus_tag="VF357_06435"
     CDS             complement(1982..>2732)
                     /locus_tag="VF357_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7523820.1"
                     /translation="KGAFTGAHAARAGKFEAAHNGTIFLDEIAEMSPPLQAKLLRVLQ
                     EKSVTPVGGNRAIPVDARVITATNKDLEEEISEGRFRSDLYFRLNVIPIRIPPLRDRR
                     DDIPLLIEHFVKKYNREKRRALEGVRPESLEILRRYTWPGNVRELENLVERIVVLKGS
                     GWLEPSDIPEKIRRAKRFLDNAIPVLGDTGLDIKSATEDFENALIRQALHLSGGNKNR
                     AATLLGLKRTTFVEMLKRKSLEAGEAPGPVS"
BASE COUNT          433 a          862 c          899 g          538 t
ORIGIN      
        1 gcgtcgaggg agaacacggt catccccatg aacgtcgcca gctgcttgcg aagctggtct
       61 acggtgaccc ccaggttctc cgcgacctct tcttcctcgg gggccctccc gaggatgttt
      121 tcgagccggg tgtaggtttg ctgcagccgg gttgagtgct gccgcgccga tcgtgggaac
      181 cagtccatct cgcggaggta gtccagcatt gccccccgga tccggaactc ggcgtacgtc
      241 ttgaacttga tcccgcggga gtcgtcgtac cggttcagcg cgtccagcag tcccacgacg
      301 ccggagctga cgaggtcgtc ggtctcgatg tggggaggaa tgcgcatctt caggcgggcg
      361 gcgtgaatgc ggatgctcgg gaggaactcg ttgacgatcg cgtcccggtc gggtttgcgg
      421 gatttccggg gggatgcctt tcggatcctg tccttggaaa gcgctgcctg tccctgcatc
      481 atggcgtttg ccctcgtctt ttggggtccg atacgaatgg tgtcgcccaa taggagagca
      541 aacggtgtgc catccggtga aaacatataa cctattgata tgtattcgtt atttattaag
      601 aggaaagatg aagaagcggg cggggcgtca atactccccg aaaaccgtca aaatcttgac
      661 ggttttgcgt cgagaacgga tcgcaccgtg cgggcgagcg tctcccgctc gtacggtttc
      721 tggaggaaat cgtcgaatcc gtccgggacg gcgccggaag gttttccctc ggcttcggag
      781 gtcccggagc agaggatcgt ccggagggtc ggacggatgg cgcggagccg cgcgagggtt
      841 tcgacaccgt tcatcccggc catgatccga tcgacgatca ccaggtccac cttttccggc
      901 gcgtagcgaa ggatctcgat cccttcctcg ccggagaccg cgggcagggt tgtgtatccc
      961 agcgatcgga gcacgtcgca cagaaggtta agggggatct cctcgtcgtc gacgacgagc
     1021 acggtctcgg tgccccgggg gatctccgtc tcggactcgc gcggttcgtc ggcatcctcc
     1081 gattcttcca tgaccgggag gaggatctgg aacgtcgtgc ctacgccgac gctgctcacg
     1141 aggcgtacga acccgccgtg ggtgcggacg cagctgtaca ccatcgagag gccgagcccc
     1201 gtcccctcgc ccggtgcctt ggtcgtgaag aagggggcga agacctcgtc gcgaatctcc
     1261 tccgggatgc cgcttccggt gtcggccacg cagatggaaa cgtaccgccc ctcgggaacg
     1321 aggacgtcgt tcaggtagac ctccccgtcg gaaacgaagg tggaggtgga gaacgtcaga
     1381 tgtcctccgc cgggcatggc gtcggaagcg ttgactccca ggttcagcag cgcctgcaca
     1441 agcgtccctg cgtcccccgt cacggtttcc tgcgggtgat ggaaatcggt gtggatctcg
     1501 acgttcttgt tcacggagcg ggcaaggatc ccgatcgcct ccccggtcac cttccggaga
     1561 tccaccggac ggaatctcgg ggtctccctg cgggaatagg cgagcagctg ccgggtcagc
     1621 tcggaggcgc ggcgggcgga ccgttcaatg taggcggccg cctcgtaccc gcggccgttt
     1681 tcgggaagga acgtcttgag gagcgatgag tagccgagaa tcccggtcag gaggttgttg
     1741 aaatcgtggg cgatcccgcc ggtcagccgc ccgatcgcct ccatcttctg cgcctgcgcc
     1801 agctgctcct cggtctggcg gacgcgggtc gaaagggcct cgatccgctc gtcccgctcc
     1861 gtggccccgg cgcgctccgc ctcgtcgagg cacgttttca gccggcgtcg gatctcctcc
     1921 cgctggtccg cgggaagacc gatcccgtcg acgacttccg cgattcggga tctgagcgag
     1981 gtcaactcac cggaccgggg gcttcgccgg cctcgaggct cttccgcttc agcatctcca
     2041 cgaaggtcgt ccgcttcagg cccagcaggg tggccgcgcg gttcttgttc ccccccgaaa
     2101 gatgcagcgc ctggcggatg agggcgttct cgaagtcctc ggtggcgctc ttgatgtcga
     2161 ggcccgtatc ccccagcacg gggatggcgt tatcgaggaa ccgtttcgcc cgccggatct
     2221 tttccgggat gtccgacggc tcgagccatc ccgaaccctt gaggacgacg atccgctcga
     2281 cgaggttctc cagctcccgg acgttccccg gccaggtgta gcgccgcagg atctcgaggg
     2341 attcgggacg caccccttcc agcgcccgcc tcttctcccg gttgtacttc ttgacgaagt
     2401 gttcgatcag cagggggatg tcgtcacgcc ggtcccggag cggaggaatc cggatgggga
     2461 tcacgttcag gcggaagtag agatcggagc ggaacctccc ctcggagatc tcctcctcga
     2521 ggtccttgtt ggtcgccgtg atgacgcgcg cgtccactgg gatcgccctg tttccaccga
     2581 ccggggtgac cgatttctcc tgcagcaccc ggagcagctt tgcctgcagc gggggactca
     2641 tctccgcgat ctcgtcgagg aagatcgtgc cgttgtgggc cgcctcgaat tttcccgccc
     2701 gcgcggcgtg ggcccccgtg aacgcgccct tc
//
LOCUS       DASVJL010000304         3825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_798898, whole genome shotgun sequence.
ACCESSION   DASVJL010000304 DASVJL010000000
VERSION     DASVJL010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798898"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..763)
                     /gene="cobO"
                     /locus_tag="VF357_06440"
     CDS             complement(155..763)
                     /gene="cobO"
                     /locus_tag="VF357_06440"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX7523821.1"
                     /translation="MSSQGKTHRRGRRMEPRKAPERIPEKPRRGLVLVITGDGKGKTT
                     SCLGMAVRAVGYGMKVLMVQFIKGSLHYGELDGAKRLAPEFELLPMGKGFVGIRGDSL
                     PMEEHVAAAREALALARERMLSGKYDVIILDEVNVAVRLGLLDVKEVLATIAEKPENV
                     HLILSGRNAHEDVIRVAHLVSEVRSVKHPYDQGIEAEKGIDY"
     gene            827..1009
                     /locus_tag="VF357_06445"
     CDS             827..1009
                     /locus_tag="VF357_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523822.1"
                     /translation="MDKNRCMACAKEGAFEGGICDLCKAIIRGEALEQHHQVKKDADR
                     SLHKEGTVIEKKQSSG"
     gene            complement(1013..2218)
                     /locus_tag="VF357_06450"
     CDS             complement(1013..2218)
                     /locus_tag="VF357_06450"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HEX7523823.1"
                     /translation="MPIVTLAPDVYCVGVNDPGLTVFDIVIPTEHGTTYNSYLVKGTE
                     KTALIDCVKRPFVGEFFRKIEEILPVEKIDYVVVNHSEPDHAGGIVDLLKRHPNVQVY
                     LSRPAKSFVDNLVNGEYKFHIVNDNEELSLGGKTLRFINAPFLHWPDTIFTYVVEDKI
                     LFPCDFLGSHYSSPHTFNDELDAPEKARSAFEFYYSTIMRPYKEHILYACERLKDLPI
                     SMIAPSHGPVLRKNPLEYVAWYKERADIMARVKEKKVPIVYVSAYGNTAAMAKKVAEG
                     VAAAGLTPVLLNAVEVPMNRIIDELDESVGFLIGTPTLNSNLPHPILSMIGHMVVLNL
                     RGKTASAFGSYGWSGEAIKMVQDILTAMRIKVSPEPIKFKMTPSGQDLDACFEFGKKF
                     AETAGGRAA"
     gene            complement(2252..2413)
                     /locus_tag="VF357_06455"
     CDS             complement(2252..2413)
                     /locus_tag="VF357_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX7523824.1"
                     /translation="MKKWRCNVCGYIYDPAAGDPANGVPPGTDFEKIPAEWVCPLCGV
                     GKEEFEAVD"
     gene            complement(2564..2671)
                     /locus_tag="VF357_06460"
     CDS             complement(2564..2671)
                     /locus_tag="VF357_06460"
                     /inference="COORDINATES: protein motif:HMM:NF045043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523825.1"
                     /translation="MKKWKCTVCGYIHQGDEPPAVCPICGAHREKFVQI"
     gene            2724..3257
                     /locus_tag="VF357_06465"
     CDS             2724..3257
                     /locus_tag="VF357_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX7523826.1"
                     /translation="MRNPTNGGRIIEIPYVAADVETTGLSAADGHRVCEFALLRFLRG
                     TVIDSFVSLVNPLRPIDPGASAVNGITDVMVAGAPTFADLLPRILDFLADDPLVFHNA
                     PFDLSFLRSEARIAGGSWPGNRVIDTLTLARRTGRFRSHSLPNICRELGIGAAFHRAE
                     ADAWAAGKLLLHLHGER"
     gene            complement(3289..>3825)
                     /locus_tag="VF357_06470"
     CDS             complement(3289..>3825)
                     /locus_tag="VF357_06470"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEX7523827.1"
                     /translation="GDSSIGMNVAAIRLGGRGVTFDVSLDVNETAAIVYDAKRRGGKS
                     AVWILGGGSPKNFMLQTEPQIQEVLGLDEKGHDYFLQITDARPDTGGLSGATPSEAVS
                     WGKVDPDKLPDTVVCYLDSTVALPLLCAFAFDRVGKRTRRRLYDRRVEMLAVLGKAYR
                     KAVNAAGVTKKRKVARKK"
BASE COUNT          648 a         1251 c         1186 g          740 t
ORIGIN      
        1 aacttcgggt ggcgcagcgc gatcaagcag ggacagctcg ccggcgagaa catggccggc
       61 ggcggcaagg tctacatcag gaacgccgag gattacttcg ggcttctgta cgggaccccg
      121 ctgctggagc gcgcgggggg ggcggcttaa acgctcagta gtcgatcccc ttctccgcct
      181 cgatcccctg gtcgtagggg tgcttgacgc tgcgcacctc gctcaccagg tgggcgacgc
      241 gaatcacatc ctcgtgcgcg ttccgtcccg acaggatcag gtggacgttt tccggcttct
      301 ccgcgatcgt cgcgaggacc tccttcacgt cgagcagccc gaggcggacc gccacattca
      361 cctcgtcgag gatgatcacg tcgtacttcc cggagagcat ccgctcccgg gccagagcca
      421 gcgcctcgcg cgccgccgcc acgtgctctt ccatcggaag cgagtcgccg cggattccca
      481 cgaacccttt ccccatcggt agaagctcga actcgggcgc cagccgtttc gccccgtcga
      541 gctcgccgta atggagcgac cccttgatga actgcaccat caggaccttc atcccgtacc
      601 cgacggcgcg caccgccatg ccgagacacg aggtcgtctt ccccttcccg tcgccggtaa
      661 tgacgagaac gagcccccgg cgcggctttt ccgggatccg ttcgggcgct tttcgaggtt
      721 ccatccttct cccccttcga tgtgtctttc cttgggatga caaccggacg gacccgattc
      781 ccggggaaga attttaatcc gtgtccccct gtgggatact attggcatgg acaagaaccg
      841 gtgcatggct tgcgcgaagg agggggcgtt cgagggaggg atctgcgacc tgtgcaaggc
      901 gatcatccgc ggcgaggccc tggagcagca ccaccaggtc aagaaagacg ccgaccgttc
      961 ccttcacaag gaagggacgg tcatcgagaa gaaacaatcc tccgggtgac ggttacgccg
     1021 cccgccctcc cgcggtctcc gcgaactttt taccgaattc gaagcaggca tcgagatcct
     1081 gcccggaagg cgtcatcttg aacttgatcg gctcgggcga caccttgatc cgcatcgccg
     1141 tcaggatatc ctgcaccatc ttgatcgcct cgccgctcca gccgtaggaa ccaaatgcgg
     1201 aagcggtttt ccctctaagg ttcaacacca ccatgtgccc gatcatgctg aggatcgggt
     1261 ggggcagatt gctgttcagg gtcggcgtgc cgatcaggaa gccgacggat tcgtcgagct
     1321 cgtcgatgat tctgttcatc gggacctcga cggcgttcaa cagcaccggc gtgagtcccg
     1381 cagcggccac cccttccgcc accttcttcg ccatcgcggc cgtgttcccg taggcggaaa
     1441 cgtagacgat cggcaccttc ttttccttca ccctcgccat gatgtccgcc cgctccttgt
     1501 accatgccac gtactccagc gggttcttcc ggagcaccgg tccatgggac ggagcgatca
     1561 tcgagatcgg gaggtccttc agtcgctcgc acgcgtagag gatgtgctcc ttgtacggcc
     1621 gcatgatcgt gctgtagtag aactcgaagg cgctgcgggc cttttcggga gcatcgagct
     1681 cgtcgttgaa ggtatggggg gacgaataat gggaaccgag gaagtcgcag gggaaaagga
     1741 tcttgtcttc caccacgtac gtgaagatcg tgtcgggcca gtgaaggaac ggcgcgttga
     1801 tgaaccggag cgtcttgccg ccgagggata gttcctcgtt gtcgttcacg atgtggaact
     1861 tgtactctcc gttgacgagg ttgtcgacga acgacttggc ggggcgggag agatagacct
     1921 gcacgttcgg atgccgcttc agcaggtcga cgatcccgcc cgcatggtcg ggctcggaat
     1981 ggttgacgac cacgtagtcg atcttctcca ccgggagaat ctcttcgatc ttgcggaaaa
     2041 actccccgac gaacgggcgc ttcacgcagt cgatgagggc ggtcttctcc gtacccttga
     2101 cgaggtagga gttgtacgtg gtaccgtgct ccgtcgggat gacgatgtcg aaaacggtca
     2161 accccgggtc gttcaccccg acgcagtaga cgtcgggcgc aagtgtcacg atgggcatct
     2221 ggaaggcacc tcgctgattt tcagaatctg gtcagtcgac cgcttcgaac tcttccttcc
     2281 ccacgccgca gagggggcat acccattcgg ccgggatctt ctcgaagtcg gtgccgggag
     2341 gcacgccgtt ggcggggtcg ccggcggccg ggtcatagat gtatccgcag acattgcatc
     2401 tccatttctt cattggacgc ccccttggcg atgatcggtt cagcgattat agcttcccgc
     2461 gcgtgcgaaa cgcaagacgg ccttcgcgag ggcgcgggag agcgccgcgc cgatccgccg
     2521 gctcccgccg tggccggcgc aaccttcccc cgggcgcccg gcatcaaatc tggacgaact
     2581 tctcgcggtg cgccccgcag atgggacaga cggcaggggg ctcgtccccc tggtggatgt
     2641 agccgcagac ggtgcacttc cactttttca tcggtgcgct cctgtgggat gatggtgccc
     2701 gtattctaca tcagggggga agattgagga acccgacgaa cggcggacgc atcatcgaga
     2761 tcccgtacgt cgcggcggac gtggagacca ccgggctttc cgcggccgac ggccaccggg
     2821 tctgcgagtt cgccctgctc cgcttcctgc gggggacggt gatcgactcc ttcgtgtcgc
     2881 tggtgaatcc cctgcgcccc atcgaccccg gcgcgtcggc ggtgaacggg atcaccgacg
     2941 tgatggtggc cggcgccccc accttcgccg accttcttcc gcgaatcctc gactttctcg
     3001 ccgacgaccc gctcgtattc cacaacgcgc cgttcgacct gtcgttcctt cggagcgagg
     3061 cgcggatcgc ggggggatcg tggcccggga accgggtgat cgacacgctg acgctggctc
     3121 gccggacggg gcgcttccgc tcccactccc tcccgaacat ctgccgggag ctcggcatcg
     3181 gtgccgcctt ccaccgggcg gaagccgacg cctgggccgc gggaaaactc ctgctgcacc
     3241 tgcacggaga aagatgactc ttttcggatc aggattttcg gctttatttc acttctttcg
     3301 cgcgaccttc cgcttcttcg tcaccccggc ggcgttcacc gccttgcggt acgcctttcc
     3361 caggaccgcc agcatctcca cccggcggtc gtagagacgc ctgcgggtcc gcttccccac
     3421 ccggtcgaac gcgaaggcgc aaaggagcgg gagcgccacg gtggagtcga ggtagcagac
     3481 gaccgtgtcg gggagtttgt ccggatccac cttcccccac gagaccgcct ccgacggcgt
     3541 cgcgccggac agcccgcccg tgtcgggccg ggcgtcggtg atctgaagga agtagtcgtg
     3601 tcccttttcg tcgagcccga gaacctcctg gatctgcggc tcggtctgga gcatgaaatt
     3661 cttcggcgac ccgccgccga ggatccagac tgcgcttttc cccccgcggc gtttcgcgtc
     3721 gtagacgatc gccgccgtct cgttcacgtc gagggagacg tcgaacgtca ccccgcggcc
     3781 gccaagacga atcgcggcga cgttcatccc gatggaggaa tctcc
//
LOCUS       DASVJL010000305         8385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_424403, whole genome shotgun sequence.
ACCESSION   DASVJL010000305 DASVJL010000000
VERSION     DASVJL010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424403"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VF357_06475"
     CDS             complement(<1..738)
                     /locus_tag="VF357_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX7523828.1"
                     /translation="MRTEGGGGETWRRLADRELSWEALRARARVLEGIRAFFRDRGFL
                     EVEPPIAQAYPNIDPNIFPVKIADASGKAARFYLHTSPELAMKKLLAAGSGDIFFLGK
                     VFRDREGSPLHSPEFTMLEWYRVGGDADTVMRDIEELVRTLARAIPREAVAHGKEKEI
                     PVDGPWPRWELDDAFRELLGAGMAEEKELREVLEHKGLRPGADESWEDLFHRACLDVV
                     EPALVECGPCFLTGYPTALGAMARRRSG"
     gene            830..1552
                     /gene="pxpB"
                     /locus_tag="VF357_06480"
     CDS             830..1552
                     /gene="pxpB"
                     /locus_tag="VF357_06480"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEX7523829.1"
                     /translation="MGAVRMLVAGEQGLVVEFGNAIDPAVNARVHRLARALATRGLEG
                     VIESIPTYRSLLVLFDPLVLPRERLVPEVEALLSIPVVEEASGAGGNTVELPVLYGGE
                     FGPDLAFIARHNGLSTEEVIAIHSSSSYLVYMLGFTPGFPYLGGMSSRLAAPRLLSPR
                     GKVPSGSVGIAGSQTGVYPLESPGGWRLIGRTPLRLFDLEAGRPFLLAPGDVVRFRPV
                     DEEEYWAYLQVEEIRAFERRGR"
     gene            1549..2511
                     /locus_tag="VF357_06485"
     CDS             1549..2511
                     /locus_tag="VF357_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEX7523830.1"
                     /translation="MIAVLASGFLTTVQDEGRRGYRAFGMPWAGAMDRYALAVANLLA
                     GNPPGAAVLEMTLSGGSLRFEEAAYAALCGADMGAALEGAPAGNWVAFPVASGATLSF
                     GTAARGCRAYLAVLGGIDAPVVLGSRSTYTRAGVGGFGGRALAGGDTLRIGPAPAVGR
                     FSPRRSLPAALVPPCGGDVRLRVLAGPQEDMFASDGIATFFSSAYTVTDRNDRMGYRL
                     EGPAIRHNGEADIITDALAPGAVQVPGDGMPIVMMADCQTSGGYAKIGTVIGPDLRLL
                     AQSRRGDSVHFLHCPEEEAITALREERAAYAAIKERFPDVGGGG"
     gene            2518..3276
                     /locus_tag="VF357_06490"
     CDS             2518..3276
                     /locus_tag="VF357_06490"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEX7523831.1"
                     /translation="MQIDLNCDMGEGYGRYVLGNDEAVIPLVSSVNVACGFHASDPST
                     MRRTVKLAKEWNVAVGAHPSFPDRVGFGRREMSASPAEIRDDVIYQLGALLAFCKAEG
                     VPLRHVKPHGALYNLAARDIEAATAIAAAVRSVDPSLYLVGLCGSAMVAAARETGLKF
                     APEAFADRGYRKDGRLVSRKEHGAVLDDPEVVAERVVRMVRDGEVITVDGTGIPIDPK
                     TVCVHGDTPGVVALLRAIRSRLEREKIGVKSFSG"
     gene            3318..4586
                     /locus_tag="VF357_06495"
     CDS             3318..4586
                     /locus_tag="VF357_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7523832.1"
                     /translation="MKRRFANVSGMVMTAALTALLAMPAAAVDKVRIGAIYPLTGPAG
                     STGAELKDAITLAADIVNGKYDLDLPLARTQGLPNLKGAQVEVIFGDHQGKPDVGQAE
                     AERMITTEKVVALMGAYHSSVTTTASIAAERHKVPFLNPESTDPKLTERGFRYFFRTT
                     PFDDLFAENFFQFLNDVKKQKGAKISSVATVYENTNFGTGVNNAIRKNAAKYGYRLVA
                     DVAYAEKGTSAIAEVQKVKAAAPDVVMPASYTADAILYMKTFKDLNYAPQAILAMDAG
                     YISDEFKKTLGKDGEYVLSREVWALDLAKKKPMIAKVNEIYKKRFGRDMNGNSARSFT
                     GFLVLADAINRAGSTDPEKIREALEKYTLGGNMMIMPWDGVAFDPKTHQNTKGRGIIV
                     QLVSGEWYTVWPFDLASRDVVWPMPAWGKR"
     gene            4607..5473
                     /locus_tag="VF357_06500"
     CDS             4607..5473
                     /locus_tag="VF357_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7523833.1"
                     /translation="MFSTELAQTLLSGVLMGLIFSLVAVGLTLIWGIMDIVNFAHGDF
                     LMSGMYISYWMYALWGIDPLLSLPVSGAFLFFLGMVAYRLVIRRILGAPMLIQIFATF
                     GIMILMRYLAFYFFKPNYRSIQQTLLSGNIDLHGVYLSIPQLAAAAGAFLTTGAVYVL
                     IHKTRVGGALQATAEDREAAALMGINTDRMFTLAWGIGAATAGIAGSLLSTFFYIFPE
                     VGGIFGLIAFVAVTLGGFGNVAGAFVAGILIGVVEAMAGYLYDPALKTVFVYVLFLGV
                     LFFRPQGLLGVE"
     gene            5474..6445
                     /locus_tag="VF357_06505"
     CDS             5474..6445
                     /locus_tag="VF357_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7523834.1"
                     /translation="MASGVAAGSGFLYARNGALLAAALAYPFLFRQPFPQDLAIKVFL
                     FGGLASAWNVLGGFAGQVSLGNAIFFGVGAYAAAVVQTQWGWTPWAGMLLGMAASAGI
                     GALIGYPCFRLKGHYFAMATLAIGEVISTLFMNWQWVGGAVGVYIPLRDDTWKYFQFG
                     TTKLPYYYVMLAFLVSALLLSAALLRSRMGYYFRAIREDQDAARSLGVPARRYKLAAM
                     GISAALTSAGGSLYAQYVLFIDPHSVFPMMLSIQVVLIAILGGVGTLWGPVVGAMVLI
                     PLSEFTRIYIGGTGSGLDLIAYGLLIIVISVIQPRGVLGFFRKAGAA"
     gene            6442..7170
                     /locus_tag="VF357_06510"
     CDS             6442..7170
                     /locus_tag="VF357_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523835.1"
                     /translation="MSFLTVGGVTKRFGGLSANDDISFEVAQGEIVGLIGPNGAGKST
                     LFSCIAGYHPVTSGTIDFDGKRISGLPPETICRAGVARTFQIVHVFKEMSVLENVMVG
                     AFLSTSSRREAEAHARAVLDRVGLSGKAEAFARSLTVSEQKRVQLARALATRPKLLLL
                     DEVMAGLTPQETAEAVGLVRSFREDGITMVIVEHVMEVVMPISDRVVVLDYGKKIAEG
                     APEEIVRNELVIKAYLGEKRGAKH"
     gene            7157..7864
                     /locus_tag="VF357_06515"
     CDS             7157..7864
                     /locus_tag="VF357_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7523836.1"
                     /translation="MLSIDRIAVDYDGVPALHDITVRVPEGAIVAVVGSNGSGKSTML
                     KTAAGLLHPSRGTITFEGADISRTPAHEIPKMGIALVPEGRKLFGKMSVRRNLDMGAY
                     TRTSRAEIERNLERVFRFFPRLSERLSQQAGTLSGGEMQMLAIGRAMMSNPRLLMLDE
                     PSLGIAPNLVDRIFEVIADLNAKENLTILLVEQNVSEALSLAHFGYVIQTGRTVLSDT
                     GKALLDSPMVRKAFLGM"
     gene            complement(7929..>8385)
                     /locus_tag="VF357_06520"
     CDS             complement(7929..>8385)
                     /locus_tag="VF357_06520"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523837.1"
                     /translation="KAGCAMCHNGPNLTDEQFHNLGVPEDPKAQEDPRVLATARFVAK
                     VSGFPDYRTLRDDPGRFLVTKDPVDWKTFATPPLREVAATAPYMHNGALRTLEEVIDF
                     YDRGGGDDPKKSPLLKPLGLTRGEKEALKDFLAGGVSGTVLEIHPPRVP"
BASE COUNT         1298 a         2643 c         2940 g         1504 t
ORIGIN      
        1 tccggagcgg cggcgcgcca tggcgccgag ggccgtcggg tacccggtca ggaagcaggg
       61 gccgcattca acgagtgccg gctctacaac gtcgaggcag gcacgatgga agaggtcctc
      121 ccacgattcg tccgctcccg gacgcaaccc cttgtgttcg agcacttcgc gcaactcctt
      181 ctcctccgcc atcccggcac cgaggagttc ccggaacgcg tcgtcgagtt cccaccgggg
      241 ccacggcccg tcgaccggga tctctttctc tttgccgtga gcgaccgcct cgcggggaat
      301 cgcccgtgcc agtgtccgga ccagctcctc gatgtcccgc atcacggtgt ccgcgtctcc
      361 gcccacccgg taccattcga gcatcgtgaa ctcgggggag tggaggggcg acccttcccg
      421 atcccggaac acctttccga ggaagaagat gtcgcccgac ccggcggcga gaagcttctt
      481 catcgcgagt tcgggggagg tgtgcaggta gaaccgcgct gccttgccgg aggcgtcggc
      541 gatcttcacc gggaagatgt tcgggtcgat gttcgggtac gcctgcgcga tcggcggttc
      601 cacctcgagg aaccccctgt cccggaagaa ggcccggatc ccctcgagta cccgggcccg
      661 cgcccgaagc gcctcccacg acagctcccg gtccgcgagc cgccgccacg tctccccccc
      721 ccccccctcc gtccgcatgc gtactgttta ccccggattc atcccgccgt aaagcaccag
      781 tgcatgaaac gcagggtacg gggctataat caggtcgtcc gggggaaaca tgggcgcggt
      841 gcggatgctc gttgcaggtg aacagggtct cgtcgtggag ttcgggaacg ccatcgaccc
      901 ggcggtcaac gcccgggtcc accggctggc ccgcgcgctg gccacgcgcg gcctggaggg
      961 cgtcatcgag tccatcccca cgtaccgatc cctcctcgta ctgttcgatc ccctggtctt
     1021 gccgcgggag cggcttgtcc cggaggtcga ggcgcttctc tcgatcccgg tagtcgagga
     1081 ggcgtccggt gcgggcggaa acacggtgga actgcctgtc ctgtacggcg gagagttcgg
     1141 tcccgacctc gcattcatcg cgcggcacaa cgggctctcc accgaagagg tgatcgcgat
     1201 ccactcctcc tcgtcctacc ttgtatatat gcttggattc acccccgggt ttccttacct
     1261 cggcgggatg tcttcccggc ttgcggcgcc gcgcctgctt tccccccggg ggaaggtgcc
     1321 gtccgggtcc gtcgggatcg cggggagcca aacgggggtg taccccctgg agagtccggg
     1381 tggttggcgg ctgatcgggc ggacgccgct gcgactcttt gatctggaag ccgggcggcc
     1441 gtttctcctc gccccggggg acgtcgtacg gttccggccg gtggacgagg aggagtactg
     1501 ggcgtacctc caggtcgagg agatccgggc gttcgagcgg aggggacggt gattgccgtc
     1561 cttgcgtccg gcttccttac gaccgtgcag gacgagggcc gccgggggta ccgggcgttc
     1621 ggcatgccat gggccggcgc gatggaccgg tatgcccttg cggtggcgaa ccttctcgcg
     1681 gggaatccgc cgggggcggc ggtgttagag atgacgctgt cgggcggatc gctccgcttc
     1741 gaggaagcgg catacgcggc gctgtgcggc gcggacatgg gagcggcgct ggagggggcg
     1801 cctgcgggaa actgggttgc cttccccgtc gcatccgggg cgacgctttc ctttgggacc
     1861 gccgcgcggg gatgccgcgc ctacctcgcg gtgctcggag ggatcgacgc gcccgttgtc
     1921 cttgggagcc gctcgaccta cacgcgtgcg ggggtcgggg gcttcggggg acgggccctc
     1981 gccgggggag acacgttgcg catcggcccg gcgccggcag tcgggcggtt ctctccccgg
     2041 cggtccttgc cggccgcgct tgtcccaccg tgcgggggag acgtccggct tcgcgttctt
     2101 gccggaccgc aggaggacat gttcgcttcg gacgggatcg ccacgttctt ttcctccgcg
     2161 tacacggtga ccgatcggaa cgaccggatg gggtaccgcc tcgaaggccc ggcaatccgg
     2221 cacaacgggg aggcggacat catcacggat gcccttgccc cgggggcggt ccaggtgccg
     2281 ggagacggca tgccgatcgt gatgatggcg gattgccaga cctcgggagg gtacgctaag
     2341 atcgggacgg tcatcggtcc ggacctgcgt ctgctggccc agtcgcggcg aggagactcg
     2401 gtccattttc tccattgccc ggaggaagag gccattacgg cgctgcgcga ggaacgtgcg
     2461 gcatatgcgg cgatcaagga acggttcccg gacgttggcg gcggagggta agagcgagtg
     2521 cagatcgacc tgaattgcga catgggggaa gggtacggaa ggtatgtcct cgggaatgac
     2581 gaggccgtga ttcctcttgt ttcctccgtg aacgtcgcgt gcggcttcca tgcctccgac
     2641 ccgtcgacga tgcgccggac ggtgaagctc gcgaaagaat ggaacgtcgc cgtcggggcg
     2701 catccctcct ttccggaccg ggtggggttc gggcggcggg agatgtcggc gtcccctgcg
     2761 gagatccgcg acgatgtcat ctaccagctg ggcgcgttgc tcgcgttctg caaggcggag
     2821 ggggtgcccc ttcggcatgt caaacctcac ggcgcattgt acaatctggc ggcgagggac
     2881 atcgaagcgg cgacggcgat cgcggcggcg gtccgctcgg tcgatccttc cctgtacctc
     2941 gtcggcctgt gcgggtccgc gatggtggcg gcggccaggg aaacggggct gaaattcgcc
     3001 ccggaggcgt tcgccgaccg cgggtaccgg aaggacggtc ggctcgtctc ccggaaggag
     3061 cacggggcgg tcctcgacga tccggaggtc gtggcggagc gggtggtccg gatggtccgg
     3121 gacggcgaag tgatcaccgt cgacgggacg gggatcccca tcgacccgaa gacggtgtgc
     3181 gtccatggcg acacccccgg ggtggttgcg ctgcttcgcg ctatccggag ccggttggaa
     3241 agggagaaga tcggggtaaa gtcgttcagc ggctgacagc ggtgggaacc accatccgaa
     3301 gaaaaggagc gagtgcgatg aaaagacgat ttgcgaacgt gtcggggatg gtcatgacgg
     3361 ccgcgctgac ggcgctgctg gcgatgccgg cggcggcggt ggacaaggtg cggatcgggg
     3421 cgatctaccc gctgaccggc cccgcggggt ccacgggggc ggagctcaag gacgcgatca
     3481 cgctggcggc ggacatcgtc aacggcaagt acgacctgga ccttcccctg gcgcgcaccc
     3541 agggattgcc caacctgaag ggggcccagg tcgaggtgat cttcggcgac caccagggga
     3601 aacccgacgt ggggcaggcg gaagcggagc ggatgatcac gacggagaaa gtcgtcgccc
     3661 tgatgggcgc gtatcacagc tccgtgacca cgacggcctc catcgcggcg gagcggcaca
     3721 aggtgccgtt cctgaacccc gagtccacgg accccaagct cacggagcga gggttcagat
     3781 atttcttccg caccacgccg ttcgatgacc tgttcgcgga gaatttcttc cagttcctga
     3841 atgacgtgaa gaagcagaag ggcgcgaaga tctcctccgt ggccacggtg tacgagaaca
     3901 ccaacttcgg cacgggggtg aacaacgcga tcaggaagaa cgcggcgaag tacgggtatc
     3961 ggctggtggc ggacgtcgcc tacgccgaga aggggaccag cgcgatcgcc gaggtgcaga
     4021 aggtgaaggc cgctgccccc gatgtcgtga tgccggcgtc ctacaccgcg gacgcgatcc
     4081 tctacatgaa gacgttcaag gatctcaact acgcgccgca ggcgatcctc gcgatggacg
     4141 cggggtacat ctccgacgag ttcaagaaga ccctgggcaa ggacggcgag tacgtcctct
     4201 cccgcgaggt gtgggcgctg gacctggcga agaagaagcc gatgatcgcg aaggtcaacg
     4261 agatctacaa gaagcgtttc ggccgcgaca tgaacggcaa ctccgcacgc tccttcacgg
     4321 ggttcctcgt gctggcggac gccatcaacc gtgcgggttc gaccgacccg gagaagatcc
     4381 gcgaagcgct ggagaagtac accctgggcg ggaacatgat gatcatgccg tgggacggcg
     4441 tggccttcga cccgaagacg caccagaaca cgaaggggcg cgggatcatc gtccagctgg
     4501 tttccggcga gtggtacacc gtctggccgt tcgacctcgc ctcccgggac gtggtctggc
     4561 ccatgcccgc ctggggcaag cggtagggag ggaggcgcgc ccggggatgt tctcgacgga
     4621 gctggcccag acgctgctga gcggggttct catggggctc atcttctccc tcgtggcggt
     4681 ggggctcacc ctcatctggg ggatcatgga tatcgtcaat ttcgcccacg gcgatttcct
     4741 gatgagcggc atgtacatct cctactggat gtacgcactt tgggggatcg accctctcct
     4801 gtccctccct gtctcggggg ccttcctttt tttcctgggg atggtcgcgt accggctggt
     4861 gatccggagg atcctgggcg cgccgatgct catccagatc ttcgccacgt tcggcatcat
     4921 gatcctgatg cggtacctgg cgttctactt cttcaagccg aactaccgct ccatccagca
     4981 gaccctgctg tcggggaaca tcgaccttca cggggtctac ctttcgatcc cgcagttggc
     5041 cgccgccgcc ggggcgttcc tcacgacggg ggcggtctac gtgctgatcc acaagacccg
     5101 ggtgggcggg gcgctgcagg ccaccgccga ggaccgggag gccgcggcgc tcatggggat
     5161 caacacggac cggatgttca cgctcgcatg ggggatcggc gcggcgaccg ccgggatcgc
     5221 cgggtcgctc ctgtcgacct tcttctacat ttttcccgaa gtagggggga tcttcgggct
     5281 gatcgccttc gtcgcggtga cgctgggcgg cttcggcaac gtggcggggg cgttcgtggc
     5341 ggggatcctc atcggggtcg tcgaggcgat ggcggggtac ctctacgacc ccgcgctgaa
     5401 gaccgttttc gtctacgtcc tgtttctggg ggttctcttc ttccggcccc aggggcttct
     5461 gggggtggag taggtggcat cgggcgtcgc agcggggagc ggcttcctgt acgcccggaa
     5521 cggggcgctc ctcgccgcag cactggcgta ccccttcctt tttcgacagc ccttcccgca
     5581 ggacctggcg atcaaggtct tcctcttcgg agggctggca tcggcgtgga acgtcctcgg
     5641 gggattcgcc ggccaggtct ccctggggaa cgccatcttc ttcggcgtgg gggcgtacgc
     5701 cgccgcggtg gtccagacgc aatggggttg gaccccctgg gcggggatgc tcctggggat
     5761 ggccgcctcc gccggcatcg gggcgctgat cgggtacccc tgtttccggc tcaaggggca
     5821 ctacttcgct atggccacgc tggccatcgg cgaggtgatc tccaccctct tcatgaactg
     5881 gcagtgggtg ggtggggcgg tcggggtgta catccccctg cgcgacgaca cgtggaaata
     5941 tttccagttc ggcacgacga agctcccgta ctactatgtg atgctcgcct ttctggtctc
     6001 cgctttgctg ctgtcggcgg cgttgctgcg ctcccgcatg gggtactact tccgcgcgat
     6061 ccgggaggac caggatgccg cccggtcgct gggggttccg gcgcgccgct acaagctcgc
     6121 cgcgatgggg atctccgcgg cgctcacgtc ggccggcggg tcgctttacg cccagtacgt
     6181 actgttcatc gacccccact ccgtcttccc catgatgctg tcaatccagg tggtgctgat
     6241 cgcgatcctt gggggcgtgg ggacgctctg ggggcccgtg gtgggggcca tggtcctcat
     6301 acctctttcg gagttcacgc ggatctacat cggggggacc ggttccgggc tcgacctcat
     6361 cgcctacggc ctcctcatca tcgtcatctc ggtgatccag ccccgcgggg tcctgggatt
     6421 cttccgcaag gcgggcgccg cgtgagcttc ctgaccgtcg gcggagtgac gaagcggttc
     6481 ggggggctct ccgcgaacga cgacatctcg ttcgaagtgg cccaggggga gatcgtgggc
     6541 ctgatcggcc ccaacggcgc ggggaagtcc accctgttct cctgcatcgc gggataccac
     6601 cccgtgacgt cggggaccat cgacttcgac gggaaacgga tctcggggct tccgccggag
     6661 acgatctgcc gggcgggggt cgcccgcacg ttccagatcg tgcacgtctt caaggagatg
     6721 tcggtactgg agaacgtgat ggtgggggcg ttcctgtcga cgtcctcgcg cagggaggcg
     6781 gaagcgcacg cccgggcggt gctcgaccgg gtggggcttt cgggaaaggc ggaagccttc
     6841 gcccgcagtc tcaccgtttc cgaacagaag cgcgtccagc tcgcccgcgc gcttgcgacc
     6901 cgccccaaac tccttcttct cgacgaggtg atggcgggcc tgaccccgca ggaaacggcg
     6961 gaggcggtgg gactggtccg ctcgttccgc gaggacggga tcacgatggt catcgtggag
     7021 cacgtcatgg aagtggtcat gccgatctcc gaccgggtcg tggtgctgga ttacgggaag
     7081 aagatcgcgg aaggcgcgcc ggaggaaatt gtccgcaacg agctggtgat caaggcgtac
     7141 ctgggggaga agcgcggtgc taagcattga ccggatcgcc gtcgactacg acggcgtccc
     7201 ggcgctacac gacatcacgg tgcgcgttcc ggagggagcg atcgtcgcgg tggtcgggtc
     7261 caacgggtcg gggaagagca cgatgctgaa gaccgcggcg ggcctcctcc acccatcgag
     7321 ggggacgatc accttcgagg gtgcggacat ctcccgcacg ccggcccacg agatcccgaa
     7381 gatggggatc gcccttgttc cggaggggcg gaagctcttc gggaagatgt cggtccggcg
     7441 caacctcgac atgggagcct acacccgcac ctcccgcgcg gagatcgaac ggaacctcga
     7501 acgggtcttc cggttcttcc cccggctctc ggagcgcctt tcgcagcagg ccgggacgct
     7561 ctcgggcgga gagatgcaga tgctggcgat cggcagggcg atgatgtcga acccgcgact
     7621 gctgatgctg gacgagccgt ccctcgggat tgcgccgaac ctggtggacc ggattttcga
     7681 ggtgatcgcc gatctgaacg ccaaggagaa tctcaccatc ctgctcgtcg agcagaatgt
     7741 gtccgaggct ctctccctgg cccacttcgg ttatgtgatc cagaccggcc gcaccgtcct
     7801 gtccgacacg gggaaggcac ttctcgactc gcccatggtg cggaaggcgt tcctggggat
     7861 gtgaacgggc gagattcttt ggcgaggcgt ctccagccgc cgctggctgt cctgattccc
     7921 gaagattttc agggcacacg tggcgggtga atttcgagga ccgtcccgga cacgccgccg
     7981 gcgaggaaat ccttgagcgc ttccttctcg ccccgggtca acccgagcgg tttcagcagc
     8041 ggcgatttct tcggatcgtc gcctccgccg cggtcgtaga agtcgatcac ctcctcaagg
     8101 gtcctgaggg cgccgttgtg catgtagggg gcggttgccg ccacctcccg caacgggggc
     8161 gttgcgaatg ttttccagtc caccgggtct ttcgtgacca gaaaccgccc cgggtcgtcg
     8221 cggagcgtcc ggtaatcggg gaatcccgac accttggcaa cgaaccgcgc ggtcgcgagc
     8281 acccgcggat cctcctgcgc cttcgggtcc tccgggacgc cgaggttgtg gaactgctcg
     8341 tcggtcaggt tcgggccgtt gtggcacatc gcgcaaccgg ccttg
//
LOCUS       DASVJL010000306         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_799812, whole genome shotgun sequence.
ACCESSION   DASVJL010000306 DASVJL010000000
VERSION     DASVJL010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799812"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VF357_06525"
     CDS             complement(<1..339)
                     /locus_tag="VF357_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX7523838.1"
                     /translation="MAMVERVEAIYQDVVKRNPGEVEFHQAVKEVLECLGPVLVKYPD
                     FTEAKIIERICEPERQTIFRVPWQDDRGQVQINRGFRVQFNSALGPYKGGLRFHPSVY
                     LGIIKFLGFEQ"
     gene            485..1501
                     /locus_tag="VF357_06530"
     CDS             485..1501
                     /locus_tag="VF357_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX7523839.1"
                     /translation="MKDRFDDRLRGIPAGALHGLLRRLSAIEAFRGRWESAPKPTTSV
                     LRRVRESVASGSAAASCRIAGESRPGAAAGYADALRAVFDGHASMPPTEDRLLALHAA
                     VFRHIPEERARAGRYKSAGLRERGDRDFLSEPVALRSPGPLLIPAQMETLAGWLPSRL
                     TGGEFHPLLVVAAYLLEFLAIRPFADGNGRVGRLLTNLLLLRCGHAYLPYGSLDAAIH
                     ARREEYYLALRRSQASRNLPHPDISPWLFAFLDALEEMQRREANEVITRLPGEEALSA
                     NQAAVMDLAAKEGEVTNRRVTAMLGLPRETAKQTLNRLVSLGALRRLGSGRATRYRPR
                     TGEP"
     gene            complement(1509..>2861)
                     /locus_tag="VF357_06535"
     CDS             complement(1509..>2861)
                     /locus_tag="VF357_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX7523840.1"
                     /translation="PQERLEAFLSAVRTVYASTMSPAALLYRAHRGLIDRDEQMAVLV
                     QRVSGAVHGHLFYPQVAGVGLSYNPYVWSEQIDPEAGVLRLVFGLGTRAVDRSDDDYT
                     RLVALNAPQRRPDAERSGSPAFSQRRVDLLDLRANRFTSERIDDVARASPDLPIDLYA
                     VRRSALPRGEGATPRPSDEGWILTFGKLLSETPFVPYLREMLGILRDAYEYPVDTEFT
                     GNFLPDGRCLVNLVQCRPLQVKEGGNIVDPPKRIPRGTLLLESRGPIIGQSSLSRLDR
                     VIFVDPAAYSALPQRDRPSVGRLVGRIVHLPGEGGDRNTLLLGPGRWGTSTPSLGVTV
                     SFAEIDKVSAICEIIGVGMPVVPDVSLGTHFFNDLVEANMLYLAVHPTRRGDSLNLKF
                     LAAARNRLAELLPEDAEWAGVVRVIDFPDAGDGRLPYLNANSFRQRVVCYLASPGKES
                     "
BASE COUNT          448 a          999 c          967 g          447 t
ORIGIN      
        1 ctgctcgaac ccgaggaact tgatgatccc gaggtagacc gaggggtgga accggagccc
       61 ccccttgtac ggcccgaggg cgctgttgaa ctgcacccgg aagccgcggt tgatctgcac
      121 ctgcccgcga tcgtcctgcc agggtacccg gaagatcgtc tgccgctcgg gttcgcagat
      181 tcgctcgatg atcttcgcct cggtgaaatc ggggtacttc accaggaccg gcccgaggca
      241 ctcgagaacc tccttgaccg cctggtggaa ctcgacctcg cccgggttcc gcttcaccac
      301 gtcctgatag atcgcctcca cgcgttccac catcgccatc gctcttctcc tcctcgcctc
      361 gtggacaatt tgcgccggga tccccgtcgc gtcctccaat gtaggcgcct catccgatgg
      421 gtcaagcaaa gaaggcgtaa tcgggtataa tcgggtatat gcatctggat accgagagcc
      481 ccccttgaag gaccggttcg acgaccggtt gcggggaatc ccggcgggag cgctccatgg
      541 cctgcttcgt cgcctgtcgg cgatcgaggc attccgcgga cggtgggaaa gcgccccgaa
      601 gccgacgacc agtgtcctcc gtcgcgtccg ggagtccgtc gcatccgggt ccgcggcggc
      661 ctcctgccgg atcgcggggg agagccggcc gggggcggcc gccgggtacg ccgacgcgct
      721 gcgggccgtc ttcgacggtc acgcttcgat gcccccgacg gaagaccgtc tcctcgccct
      781 tcacgcggcg gtgttccggc atatccccga agaaagggct cgcgcgggcc ggtacaagtc
      841 ggccggactt cgcgaaaggg gcgatcggga cttcctgtcg gaaccggtcg cccttcgctc
      901 ccccggcccc ctcctgatcc ccgcgcagat ggaaaccctc gcggggtggc tcccctcgcg
      961 ccttacgggt ggggagttcc accccctgct cgtcgtcgcg gcgtacctcc tggagttcct
     1021 cgccatccgg cccttcgccg acggaaacgg gcgggtgggc cgccttctca cgaaccttct
     1081 gctccttcgg tgcgggcacg cgtatttacc gtacggctcc ctcgacgcgg cgatccacgc
     1141 ccgcagggag gagtattacc tcgcgctccg ccgaagccag gcgagccgga acctgccgca
     1201 tcccgacatt tcgccgtggc tgttcgcctt tctcgacgcg ctcgaggaga tgcagcggcg
     1261 cgaggcgaac gaggtcatca cgcggcttcc cggcgaggaa gcgctgtcgg cgaaccaggc
     1321 cgccgtgatg gacctcgccg cgaaggaggg ggaagtgacg aaccgccggg tgacggcgat
     1381 gctcggcctc ccccgcgaaa cggcgaagca gacgctcaac cgcctggttt ccctcggagc
     1441 gctgcggcgg ctgggatccg gacgggcgac acggtaccgc ccccggacgg gagaaccata
     1501 gtggttccct acgattcctt ccccggggag gcaaggtagc agacgacgcg ctggcggaag
     1561 gagttggcgt tcaggtacgg aagccgtccg tcccccgcgt ccgggaaatc gatgacgcgc
     1621 acgacccccg cccactccgc gtcctccggg agcagttcgg cgaggcggtt ccgggcggcc
     1681 gcgaggaact tcaggttcag ggaatccccg cgccgcgtcg ggtggaccgc caggtagagc
     1741 atgttcgcct ccaccaggtc gttgaaaaag tgggtcccga gcgacacgtc ggggaccacg
     1801 ggcatcccca ccccgatgat ctcgcaaatc gcggacacct tgtcgatctc ggcgaacgac
     1861 accgtcaccc cgagggacgg cgtgctcgtc ccccagcgcc cggggcccaa tagcagggtg
     1921 ttccggtccc ccccttcccc ggggagatgg acgatccggc cgacaagacg tccgacggag
     1981 gggcggtccc tctgggggag cgccgagtag gcggcggggt cgacgaagat cacgcggtcg
     2041 aggcgggaca gggagctttg cccgatgatg ggcccgcgcg actcgagcag gagggtgccc
     2101 cgggggatcc gcttcggcgg gtcgacgatg ttccccccct ccttcacctg cagcggccgg
     2161 cattggacaa ggttgacgag gcaccgcccg tccggaagga agtttcccgt gaactcggtg
     2221 tccaccgggt attcgtaggc gtcccggagg attcccagca tctcccgcag gtacgggacg
     2281 aacggcgtct ccgacaggag tttcccgaac gtgaggatcc acccttcgtc ggacggccgg
     2341 ggagtcgctc cctcgccgcg cggcagggcg ctccggcgca cggcatagag atcgatcggc
     2401 aggtcggggg aggcgcgcgc cacgtcgtcg atcctctccg acgtgaaccg gttcgccctc
     2461 aggtcgagga ggtccacccg gcgctgtgag aaggccgggg agccggatcg ttccgcatcg
     2521 gggcggcgct gcggcgcgtt cagcgccacc aggcgggtgt agtcgtcgtc ggaccggtcg
     2581 accgcccggg tccccaaccc gaagaccagg cgcaacaccc cggcttctgg gtcgatctgc
     2641 tcgctccaga catatgggtt gtacgacagg cccacccccg ccacctgcgg atagaacagg
     2701 tgcccgtgga ccgcccccga cacgcgctgg acgaggaccg ccatctgctc gtcccggtcg
     2761 atcagccccc ggtgcgcgcg gtacagaagc gccgcggggc tcatcgtgct ggcatacacg
     2821 gtgcggaccg ccgagaggaa cgcttcgagg cgctcctgcg g
//
LOCUS       DASVJL010000307         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_611867, whole genome shotgun sequence.
ACCESSION   DASVJL010000307 DASVJL010000000
VERSION     DASVJL010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611867"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="VF357_06540"
     CDS             <1..1507
                     /locus_tag="VF357_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523841.1"
                     /translation="RLPTEGYFTEAKPPPEPEKTMIAMEPEKAMSAASEPVKANITPE
                     PAATPGQSADPSKGPTPEKIAPLEVGLAAVPPLAKATSAPEVPPAPVLTRDIAAGPGQ
                     DALAAGKPEAELQVPKPVSPAASVVETGKAKPAEETITLPTRSPYRGLLSDLFLAIGE
                     KWVDRGTMYLPLPSGGDAVLQLSDFPVVRFSGGAEALIDFRGGIPLRVADAISAQWKS
                     LRVVSLADAGGAGDRIDRILRVSGYHSVKEGISRPVVMGETVSVALPARWVIQRTRDS
                     LLSGDLILLKEVPEKPDPELLAVLRYARRVGLRVLPYADDPNAMEGFLVGFQEAEGGI
                     PVALVVPKTGGLPAVDFGLSLLGIAAKTGERLRLGGEGDGFRLVVSPERLFEVGGKKY
                     VVDTGKMSPAIRSILRESGYAIFPAGKDASGREIIQRLMKAAGVESEERKEYLFTGGA
                     KAGYEIRVTGAFLSMPGPSGGAGRRIVLVRRKMHSATRALLGDMGVEIVEW"
     gene            1599..2156
                     /locus_tag="VF357_06545"
     CDS             1599..2156
                     /locus_tag="VF357_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523842.1"
                     /translation="MDDASSLAGHLEFRPRLTVVLVFLTLMVAVVGWRIARDTGARRV
                     DVLAREAIGLFDAAPSTGTATAPLDAAEAEMRFRGLAGVPIGFPRDDVDFVPIEVRKE
                     TFANHPAASLRFLYAGEGYLLVVSARDRLLGTPSVAAFPEGSFVSGERDGRAFVLWER
                     EGATCIVVSEVDVTQAFDLVRRLFT"
     gene            2194..>2444
                     /locus_tag="VF357_06550"
     CDS             2194..>2444
                     /locus_tag="VF357_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7523843.1"
                     /translation="MNVAKRMKRNPVFVDEGDSMKKAMDLLKEHEIRHLPVLRDGERL
                     VGILSERDIKQASPSPATALEIREIYYLLDKVKVKQIMTR"
BASE COUNT          383 a          769 c          877 g          415 t
ORIGIN      
        1 ccgtcttccg acggaggggt acttcacgga ggcgaagccg ccgccggagc cggaaaagac
       61 gatgatcgcc atggaaccgg aaaaggcgat gtccgccgcg tcggaaccgg tgaaagcgaa
      121 catcaccccg gaacctgccg ccacgccagg gcaatctgcc gacccgtcga aaggcccgac
      181 gccggagaaa atcgcgccgc tggaggtcgg gctcgcggcc gtcccgcccc tggcgaaggc
      241 tacgtccgca ccggaggttc ctcccgcgcc ggtgttgacg cgcgatatcg ccgccggtcc
      301 gggccaggat gccttggccg ccgggaagcc ggaggcggag ctgcaggttc ccaagcccgt
      361 ctccccggcc gcgtcggtcg tggagacggg gaaggcgaag cccgcggagg agaccatcac
      421 cctcccgacg cggtctccat accgggggct cctgtcggac ctcttcttgg cgatcggcga
      481 aaagtgggtc gatcgtggaa cgatgtacct tcccctccct tccgggggcg acgcggtgct
      541 gcaactgtcc gatttccccg tcgtgcgatt ctccggggga gcggaggcgc tcattgattt
      601 ccgcggcggt atcccgctgc gggtcgccga cgcgatctcc gcccaatgga aatcgctccg
      661 ggtcgtctct ctcgccgacg cgggcggcgc gggggatcgg atcgaccgga tcctgcgcgt
      721 gtcggggtac cattccgtga aggaggggat ctcccgcccc gtggtgatgg gcgagaccgt
      781 atcggtcgcg ctccccgcgc gctgggtcat ccagcggaca agggatagcc tcctctccgg
      841 cgacctcatc ctgctgaaag aggtcccgga aaagcccgat cccgagcttc tcgccgtcct
      901 gcggtacgcc cgtcgcgtcg gacttcgcgt ccttccctac gccgacgacc cgaacgcgat
      961 ggaagggttt ctcgtgggtt tccaggaggc ggagggaggg atcccggtgg cccttgtggt
     1021 tccgaagacg ggcgggctcc cggcggtcga cttcggcctc tccctcctcg ggatcgccgc
     1081 gaagaccggg gagcgactgc gcttgggcgg ggaaggagac ggcttccggc tcgtggtttc
     1141 gcccgagcgg ctcttcgagg tcggcgggaa gaagtacgtg gtcgatacgg ggaagatgtc
     1201 ccccgcgatc cgctcgatcc tgcgggaatc ggggtacgcc atcttccccg cgggaaagga
     1261 tgcttccggc agggaaatta tccaacggct gatgaaggct gcgggggtcg agtcggaaga
     1321 gcggaaggag tacctgttca cggggggtgc gaaggcggga tacgagatac gggtcacggg
     1381 cgcgttcctt tccatgcccg gtccctcggg cggggcggga cgaaggatcg tgctcgttcg
     1441 ccggaaaatg cactcggcca cccgggcgct gctcggcgac atgggggtgg aaatcgtcga
     1501 gtggtagtcg tacaattata atttgtggaa cgcggcggac gggcgtcgga gggcattgat
     1561 cgatggggag tgcgacgaac cgagacccgg cgcgccggtt ggatgacgcg tcgtccctgg
     1621 cggggcatct ggagtttcgc ccccggctga cggtcgtcct cgtttttctc accctcatgg
     1681 tcgccgtcgt cggttggcgg atcgcccgtg acacgggagc ccgccgggtc gacgtccttg
     1741 cgcgcgaggc gatcgggctg ttcgatgccg ctccgtcgac gggaacggcc accgcgccgc
     1801 tggatgccgc ggaagcggag atgcggttcc ggggcctcgc gggcgttccc atcgggttcc
     1861 cccgggacga cgtcgacttc gtccccatcg aggtccgcaa ggagacgttt gcgaaccacc
     1921 cggccgcctc gctgcggttc ctctatgcgg gagaggggta tcttctcgtg gtgtccgccc
     1981 gggatcggct cctcgggact ccctccgtcg cggcgttccc cgaggggtcg ttcgtctcgg
     2041 gggagcggga cggcagggcg ttcgtcctgt gggagcggga gggtgctacc tgcatcgtcg
     2101 tttcggaggt cgacgtgacg caggcgtttg acctggtccg tcggctgttc acctgatccg
     2161 gagcagaacc gaattcgcca aggagggact acgatgaacg tcgccaagcg gatgaagagg
     2221 aaccccgtgt tcgtcgacga gggggactcg atgaagaagg cgatggacct gctgaaggag
     2281 cacgagatcc ggcacctccc cgtcctgaga gacggcgagc ggctcgtcgg catcctctcg
     2341 gagcgggaca tcaagcaggc gtccccttcc ccggcgaccg cgctcgagat ccgggagatc
     2401 tactaccttc tcgacaaggt caaggtgaag cagatcatga cgcg
//
LOCUS       DASVJL010000308         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1176762, whole genome shotgun sequence.
ACCESSION   DASVJL010000308 DASVJL010000000
VERSION     DASVJL010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176762"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VF357_06555"
     CDS             <1..1067
                     /locus_tag="VF357_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523844.1"
                     /translation="FFFYAYDAGFEIALEEARPLCEASDSPGIAGLRPAPAHLQYRPR
                     PLTVPAGAVPIPVSGKMYRLDATVKIFDFGVLSVTLTLPLEDLSLDEYAEIALSLASE
                     SGMEKSALDAARRLFERIRPAVSRPMFADLVEDYCVWHVGGFSPGLSGTEALARLPRE
                     IARLLTLERGELSEAAVAEIVRSPIRYFDNDLFLAEWNASFVYDPKFQDTAEVLEFLN
                     IQVLELRFFDRLLHEAIEGVSEELAPRRRLFHILHDPYEVPLRKLSGIRTDVSLIRER
                     IYNAMKLVGDAYLARVYEEARRKVGAEKHEGMVHDQLATLTDIYTVLNNQAAAARAET
                     LELIIILLIVFEIVMGLLRW"
     gene            1326..>1782
                     /gene="pgk"
                     /locus_tag="VF357_06560"
     CDS             1326..>1782
                     /gene="pgk"
                     /locus_tag="VF357_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX7523845.1"
                     /translation="MKIRTIDQLDLSGKRTLVRVDFNVPIDKAGNVTDDTRLQAALPT
                     IRLAIERGAKTILVSHLGRPKGKRLPEMSLAPVAPCLSRLLGQPVAFAQDCVGEPASM
                     AVAAMKPGDVLLLENVRYEAGEEKNDEELGKRLAALCDVYVNDAFATAHR"
BASE COUNT          308 a          580 c          594 g          300 t
ORIGIN      
        1 tgttcttctt ctacgcctac gacgcaggct tcgagatcgc gttggaggag gcgcgccccc
       61 tgtgcgaagc gtccgattcc ccggggatcg cgggtctgcg cccggcgccc gcacacctgc
      121 agtatcgtcc gaggccgctc acggtgcccg ccggggcggt tcccatcccg gtttccggga
      181 aaatgtaccg gctcgacgcg acggtcaaga tcttcgactt cggggtcctc tccgtgacgc
      241 tcaccctccc cctcgaagat ctctctctcg atgagtacgc ggagatcgca ctttccctcg
      301 cctcggaaag cgggatggag aagtccgccc tggatgcggc gcggcgactg ttcgagcgga
      361 tccgccccgc cgtctcccgc ccgatgttcg ctgacctggt ggaagactat tgcgtgtggc
      421 acgtgggcgg gttctccccg ggattgagcg ggacggaggc gctcgcccgt cttccgaggg
      481 agatcgcgcg cctgctgacc ctcgaacgag gggagctgag cgaagcggcg gtcgccgaaa
      541 tcgtccgctc accgatacgg tacttcgaca acgacctgtt cctcgcggag tggaacgcct
      601 ccttcgttta cgacccgaag ttccaggaca cggcggaggt gctcgagttc ctgaatatcc
      661 aggtgctcga gctgcgcttc ttcgaccgcc tcctccacga ggcgatcgaa ggggtgtccg
      721 aggagctcgc cccccgccgc cggctcttcc acatcctcca cgacccgtac gaggtcccgc
      781 tgcgcaagct ttccgggatc cggaccgacg tctccctgat ccgggaacgg atctacaatg
      841 cgatgaaact cgtgggggac gcctacctcg cccgggtgta cgaggaggcg cgccggaagg
      901 tgggggcgga gaagcacgag gggatggtgc acgaccagct cgccaccctc acggatatct
      961 acacggtgct gaacaaccag gcggcggcgg cgcgtgcgga gacgctcgaa ctgatcatca
     1021 tcctcctcat cgttttcgag atcgtcatgg gcctcctgcg ctggtagccg taacaaggac
     1081 cctgcgccca tcaatcgcgc aggagtgacg cagcgggggg ttccgcggag cggcgtctgg
     1141 agcgaagtgg agacaggggc gtcgggaacg agcggaggcg caggcgagga gaccatggac
     1201 ggtcgacaag ccgtagcgga gcggaagccc ccgcgaggat ccctgcgccc atgattcgca
     1261 ccgggttttt tggtaatatt cgtatcgtac gctcctcaac cgttgaccca aggagggttt
     1321 ccgagatgaa gatccgcacg atcgaccagc tggacctttc ggggaaaaga acgctcgtcc
     1381 gcgtcgactt caatgtgccg atagacaagg ccggcaacgt gaccgacgac acgcggctcc
     1441 aggcggcgct ccccacgatc cgcctggcga tcgagcgggg ggcgaaaacg atcctggtct
     1501 cccacctcgg ccgcccgaag gggaaaagac tccctgaaat gagcctggct ccggtcgcgc
     1561 cgtgcctgtc ccggctgctc gggcagccgg tcgcgttcgc gcaggattgc gtgggcgagc
     1621 cggcttcgat ggccgtggcc gcgatgaagc ccggggacgt cctgctcctc gagaacgtgc
     1681 gctatgaggc gggggaggag aagaacgacg aggagctggg caaacggctc gccgccctct
     1741 gcgacgtcta cgtcaacgac gccttcgcca cggcgcatcg gg
//
LOCUS       DASVJL010000309         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_800580, whole genome shotgun sequence.
ACCESSION   DASVJL010000309 DASVJL010000000
VERSION     DASVJL010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800580"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VF357_06565"
     CDS             <1..706
                     /locus_tag="VF357_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7523846.1"
                     /translation="CPSALLSCPIGTLQHFAAAGNVSWYAVGTLSVVGASVGRMTCGT
                     LCPFGALQDLLYKFRGWKASLPHWTRYLRYAVLAGLVFVIPWLTHENWFSKLCPVGTL
                     MGGLPWVTMNAGIRSMVRSLFWVKIAILLFFVTTATMVKRPFCRAICPLGAIFSLFNK
                     SSFLQLAWDPDTCTRCGKCQMICPVDIRPDRNPTDPDCLRCLDCTRCPSLKLTTVFHR
                     QPFAGTVMPTTSARVL"
     gene            816..2711
                     /locus_tag="VF357_06570"
     CDS             816..2711
                     /locus_tag="VF357_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523847.1"
                     /translation="MAEPVPAAGGASRRWWDLTPKEQNLVAVTLLFLVTLPMLTKIFT
                     SDFGTHIAIGREIVQTRTIADKEFLNYPSLGRENPNGEWGFQAILYLVFSVAGVYGVS
                     FFVWAIVFAIFLLLHRGAVLRGAHPLLAVLAIFAFSGFLRIRIQPRPEIFTYFFIALT
                     IFLFSEYFFGTRKWFIYLFPPLVLVWANSHPTYLMAFLLCGAFVADRLAHAIWRKEFR
                     WDTLKTWIFPPVFVGLAGLILCGLNPHGYNAILTPLRLLARGAGGGSGGSILMSISEL
                     TPVKGTGFFVYYKAAAAFAVVSLLLGLAGRKVYLLDLFLFTIAFKGAWDSARAVSMMG
                     LFLSQGTSLHLTGFFSRLPEWFPGATVGKAKLPPKEKQRRKEKAPHRETPVPERRRRT
                     LGPVVIASLAMFALIAFGGTTLLFSFSQLEYGLGMTEHKFSFPAAEFLRRNPVPGKMF
                     NFFDIGGFLDWKLYPQALTFIDGRTYNQEVFMEHQVVTGALPGWERVLEKYGINYIVL
                     KSMDSSGMILPIVPVLANDSHWALVFSDGLFLVFVRNVPELREYIKAHEIPKGILPQH
                     IIQEAYHYIFLGVSPVTAYQTMSDMFLLLGQRDQAIWALREALDTVDDPYLRARLMQL
                     QGGVGTR"
BASE COUNT          430 a          878 c          818 g          599 t
ORIGIN      
        1 ctgcccctcc gccctgctct cctgtcccat cggcaccctg cagcatttcg cggcggccgg
       61 gaacgtctcc tggtacgcgg tcgggaccct ttcggtcgtc ggcgcgtccg tggggcgtat
      121 gacgtgcggg accttgtgcc ccttcggcgc cctccaggat cttctgtaca agttccgggg
      181 gtggaaggcg tccctgccgc attggacgcg gtatctccgc tatgccgtcc tggccggcct
      241 cgtgttcgtg atcccatggc tcacccatga gaactggttt tcgaaactat gtcccgtggg
      301 gacgctgatg ggcggcctgc cgtgggtcac gatgaacgcc ggcatccgct cgatggtccg
      361 gtcgctgttc tgggtcaaga tcgcgatcct cctcttcttc gtcacgacgg cgacgatggt
      421 caagcgcccc ttctgccggg cgatctgccc cctcggggcg atcttctccc tgttcaacaa
      481 gtcgagcttt ctgcaactcg cctgggatcc cgacacctgc acgcggtgcg ggaagtgcca
      541 gatgatctgc ccggtcgaca tccgtcccga ccggaacccg accgacccgg actgcctgcg
      601 gtgcctcgat tgcacccggt gcccgagcct caagctcacc acggtatttc accggcagcc
      661 gttcgccggg acggtcatgc cgacgacctc cgcccgcgtc ctgtgatatc tttgggatcg
      721 tccgagggcg accgggaccc cggaaacgcc cgatggagat gaacgtgttc gcaacgttta
      781 cggaggggaa gatgcgcgtc gaagggggaa aatagatggc ggaacccgtg cctgcagccg
      841 ggggcgcgtc ccgccgctgg tgggatctca ccccgaagga acagaacctc gttgccgtca
      901 ccctcctctt ccttgtcacc cttccgatgc tcacgaagat cttcacgagc gacttcggga
      961 cgcacatcgc catcgggcgg gagatcgtgc aaacccggac catcgcggac aaggagtttc
     1021 tgaattaccc gtccctcggg cgggagaatc cgaacggaga gtggggattc caggcgatcc
     1081 tttatctggt gttttccgtc gcgggcgttt acggcgtctc gttcttcgtc tgggcgatcg
     1141 tgttcgcgat cttcctcctt ctgcaccggg gggccgtcct gcggggggcg caccccctcc
     1201 tggcggtcct ggccatcttc gcgttttccg ggttcctccg gatccgcatc cagccgcgac
     1261 cggagatctt cacgtacttc ttcatcgcgc tgacgatctt cctgttcagc gaatatttct
     1321 tcgggacgag gaagtggttc atttacctgt ttccgcccct cgtgctcgtc tgggcgaact
     1381 ctcacccgac gtatctgatg gcgttccttc tgtgtggagc gttcgtagcg gatcgattgg
     1441 cccacgcgat ctggcgaaaa gagttccggt gggacacttt gaagacgtgg atttttccac
     1501 ccgtctttgt gggcctggcc gggttgatcc tgtgtggtct gaatccccat gggtacaacg
     1561 cgatccttac tccgctccgc ctgcttgcca ggggggccgg agggggaagc ggcggttcca
     1621 tcctgatgtc gatctccgaa ctgaccccgg tgaagggcac cggtttcttt gtttactata
     1681 aagcggccgc ggcgtttgcc gtcgtctccc tgctcctcgg attggccggg cggaaggtat
     1741 acctgctgga cttgttcctg tttaccatcg ccttcaaggg ggcctgggat tccgcgcgcg
     1801 ccgtgtcgat gatgggactg ttcctctcgc aggggacatc gctccatctc accggcttct
     1861 tttcccggct gccggagtgg tttcccggcg caaccgtggg aaaggcaaag ctccccccca
     1921 aagagaagca gcgaaggaag gagaaggctc cgcatcgtga aacgcccgtg cccgaacgac
     1981 ggcgccggac cctcggcccg gtcgtcatcg cttccctggc catgttcgcc ctgatcgcct
     2041 tcggcggcac cacgctcctc ttctccttct cgcagctgga gtacgggctt gggatgaccg
     2101 agcacaaatt ttccttcccc gcggcggagt tcttgaggag gaacccggtc cccggcaaga
     2161 tgttcaattt cttcgacatc ggcggattcc tcgactggaa gctataccca caggcactca
     2221 cctttatcga cggccgcacc tacaaccagg aagtcttcat ggaacaccag gtcgttacgg
     2281 gggcgctccc ggggtgggag agggtcctgg agaaatacgg catcaactat atcgttctga
     2341 aatcgatgga ctcctccggg atgatcctgc cgatcgtgcc ggtcctcgcc aacgactccc
     2401 actgggcgct ggtcttctcg gacggcttgt tccttgtttt tgtccgcaac gtgcccgaac
     2461 tgcgggagta catcaaggcc cacgagatcc cgaagggaat ccttccgcaa cacattatcc
     2521 aggaggcgta ccactacatc tttctcgggg tctctcccgt gaccgcctac cagacgatga
     2581 gcgacatgtt ccttctgctg gggcagcggg accaggcgat ctgggctctc cgggaggctc
     2641 tggatacggt ggacgatcct tacctgcgcg cccgtctcat gcagcttcag ggaggcgtgg
     2701 gtacccggtg aaaggcgctc tccag
//
LOCUS       DASVJL010000310         4276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_895787, whole genome shotgun sequence.
ACCESSION   DASVJL010000310 DASVJL010000000
VERSION     DASVJL010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895787"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..937)
                     /locus_tag="VF357_06575"
     CDS             complement(95..937)
                     /locus_tag="VF357_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7523848.1"
                     /translation="MMSGPEGSSWKERRESMGKTIGQVSADLRIGHRYLAGIEEGSFE
                     DFPERVFSTGFIRSYAKYLSQDPGPVLAEYERSIGSDDDSEMAAQLRFGWVERERERG
                     SRRATYTVAAGAVLLVGVILAWITLRTERRPLPPVPPAAVALPSPPAVENATKTGATA
                     PVAAPPGVDNLAAPVPPPTPTPPTPAEPSSSVAAVGATGPLVGPFQLFLEASEQAWVM
                     YSFDDSDPIDVTLYAGDKVSIHATRRITLKLGNAGGVAGTLNGRRLPPFGERGQVRKF
                     AFGQ"
     gene            complement(934..1707)
                     /locus_tag="VF357_06580"
     CDS             complement(934..1707)
                     /locus_tag="VF357_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7523849.1"
                     /translation="MIRPPKRPFLPVVCLFAAILAGCAAPSADRKKEADARMRMGVTY
                     LEQRNLPMAMRELAKASELDPGNPEVDLALGLAYQARGDLSKAEEYLRTAIGKKPDYA
                     DARNNLGIILAERKAWDEAIREFEAAAANVMYTTPERAYFNLGEAYRAKGDPASAEGA
                     YRRALKANDRYAPAYAALSGVLGGQGKWNEAASILTRCVEILPDYAPGWMELGRVYLR
                     LSRPADALKAFNKVLAVSSDPEERKQAAGYVTLLGSEKR"
     gene            complement(1704..2561)
                     /gene="mtnP"
                     /locus_tag="VF357_06585"
     CDS             complement(1704..2561)
                     /gene="mtnP"
                     /locus_tag="VF357_06585"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX7523850.1"
                     /translation="MTDILGVIGGSGLYEMEGMKNVRRVVVRTPFGAPSDAITVGELE
                     GRTLAFLPRHGKGHRFSPSQINYRANVYAMKKIGAGAILSISAVGSMKERIRPGDIVV
                     VDQFFDHTRLRPNTFFSDGVAGHVAFADPVCPDLTAVVYTAARKVVRRVHRGGTYLCM
                     EGPAFSTRAESGIYRKWGVDVIGMTNMPEAKLAREAEICYATLALATDYDCWHETQED
                     VSIDAILDVLRRNVENSKRIVREVALRLPLPGGCRCGEALKYAIITDRKRIPPAARRR
                     LALLIGKHL"
     gene            complement(2767..3873)
                     /gene="rlmN"
                     /locus_tag="VF357_06590"
     CDS             complement(2767..3873)
                     /gene="rlmN"
                     /locus_tag="VF357_06590"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX7523851.1"
                     /translation="MISTDAERTDLKGMTLPELEGFLARWGKERYRARQLFRWIYQKH
                     AGDFAAMTDLSKELRGILAASCRISGFPAESVETSADGTEKYLFRLEDGEAVESVLIP
                     DEGRRTLCISSQVGCPLLCGFCATGAAGIRRNMTSAEIVQQACFAAKRLAEREERLSN
                     VVFMGMGEPLLNVPEVSRTIGILLSQFGFGISGKRVTVSTAGIVPEMLPLALMYPVSF
                     AVSINAARDDLRSLLMPINRKYPLKEVVAAMRRIPLQSGRKVTAEYVLLAGVNDSPED
                     ALALSRLFRGARIKVNLIPYNPHEASPYRAPVAEVVDRFRNVLVGGGVQTITRERRGA
                     DIRAACGQLRTAAGGSGGVAAGGGAPHSRTNYTP"
BASE COUNT          665 a         1506 c         1405 g          700 t
ORIGIN      
        1 gcgtcccggg tgaagaacgt gaggcggcgg tcggcctccc cgaggtcgat ggcgcgcgcg
       61 aggaatgccg gagaggagtg gaaggaaggc cggatcactg gccgaaggcg aacttccgga
      121 cctgcccccg ttccccgaac ggcggcagcc gccggccatt cagcgtcccc gccacgcccc
      181 ctgcgttgcc gagtttcaag gtgatccgtc tcgtcgcgtg gatgctgacc ttgtcaccgg
      241 cgtacagtgt cacgtcgatc gggtcgctgt cgtcgaagct gtacatcacc cacgcctgct
      301 cgctcgcctc gaggaacagc tggaaggggc cgacgagggg gccggtggca cccaccgccg
      361 ccaccgaaga ggacggttcc gccggagtcg gaggggtcgg cgtcggggga gggaccggcg
      421 ccgccaggtt gtcgacgccg ggaggggcgg cgaccggcgc ggtggcgccc gtcttcgtcg
      481 cgttctcgac cgccggcggg gaaggaagcg cgacggcggc gggcggtacc ggcggcaacg
      541 gacggcgctc ggtgcggagg gttatccagg cgaggatcac cccgacgagg agaacggccc
      601 cggcggcaac ggtgtacgtc gcccggcggc tcccccgctc acgctcccgc tccacccagc
      661 cgaaccgcag ctgcgccgcc atttcgctgt catcatcgct tccgatcgac cgctcgtact
      721 cggcgagaac gggccccgga tcctgcgaca ggtacttggc atacgagcgg atgaagccgg
      781 tggaaaatac gcgctcgggg aaatcctcga agctcccctc ctcgattccc gcgagatacc
      841 gatggccgat ccgaagatcg gcggaaacct ggccgatcgt cttccccatc gactcccggc
      901 gttccttcca ggaggaaccc tcgggcccag acatcatcgc ttctccgatc cgaggagcgt
      961 cacgtagccg gccgcctgtt ttctttcttc gggatcgctc gaaaccgcga gaaccttgtt
     1021 gaacgccttc aacgcatccg ccggccgcga gaggcggagg tagacgcgcc ccagttccat
     1081 ccagcccggg gcgtagtcgg gaaggatctc cacgcaccgg gtgaggatgg acgccgcctc
     1141 attccacttc ccctgtccgc cgaggacccc ggagagcgcg gcatatgccg gggcgtaccg
     1201 atcgttcgcc ttcaacgccc gccggtacgc tccttccgcg ctcgccggat cccccttggc
     1261 gcgatacgcc tcgccgaggt tgaaatacgc ccgttccggg gtcgtgtaca tcacgttcgc
     1321 cgcggccgcc tcgaactcgc ggatcgcctc gtcccacgcc ttgcgctccg cgaggatgat
     1381 gccgaggttg ttccgcgcgt ccgcataatc cggcttcttg ccgatcgccg tgcggagata
     1441 ctcctcggcc ttcgacagat cgccgcgcgc ctggtaggcg aggccgaggg ccaagtcgac
     1501 ttccgggttg cccggatcaa gttccgatgc cttcgccagc tcccgcatcg ccatcgggag
     1561 gttccgctgc tcgaggtagg tgacgcccat ccgcatccgc gcgtcggcct ccttcttccg
     1621 atccgcggag ggcgcggcgc agcccgcgag gatcgccgcg aagaggcaca ccacggggag
     1681 gaagggcctt ttcggggggc ggatcacagg tgcttcccga tcaggagggc gaggcgccgg
     1741 cgcgccgccg gcgggatccg cttccggtcc gtgatgatcg cgtatttcag cgcctccccg
     1801 caccggcacc ccccaggaag aggaagccga agggcgacct cccggacgat ccgcttcgag
     1861 ttctcgacgt tgcggcgcag gacgtcgaga atcgcgtcga tcgacacatc ctcctgcgtc
     1921 tcgtgccagc agtcgtagtc ggtcgccagc gcgagcgtcg cgtagcagat ctccgcttcc
     1981 cgggcgagtt tcgcctcggg catgttcgtc atcccgatga cgtcgacccc ccacttccgg
     2041 tagattccgg attcggcgcg cgtcgagaag gcgggcccct ccatgcaaag gtatgtcccc
     2101 ccccggtgga cgcgccgcac gaccttgcgc gccgccgtgt acaccacggc ggtgaggtcc
     2161 gggcagaccg ggtcggcgaa ggcgacgtgt cccgccacgc cgtcgctgaa aaaggtgttc
     2221 ggccggagcc gcgtgtgatc gaagaactgg tcgacgacga cgatgtcgcc cggccggatc
     2281 cgttccttca tgctgccgac cgccgagatc gacaggatcg ctccggcgcc gatcttcttc
     2341 atcgcgtaga cgttcgcgcg gtaattgatc tgcgaggggg agaatcggtg ccccttcccg
     2401 tggcgcggca ggaaggcgag cgttctcccc tcgagctcgc ccacggtgat cgcgtcggac
     2461 ggcgcgccga acggcgtccg gacgacgacc cgccggacgt tcttcatccc ctccatctcg
     2521 tagaggccgg aaccgccgat gacgccaagg atgtcggtca tgaaaccttc cttccaccgc
     2581 ttgggataac ctataagact ataggcagat cgggaacggc gctcaaccca tttttccctc
     2641 cgggactcac ccttgggctg cgtcgcctcg gagggcgggg ctccgttcgt ggctcgccgt
     2701 gcgatgaacc tgcacggctg cgcaccggcc tcactgtgcc ccccgttctt actactcggg
     2761 ggtacctcag ggagtgtagt tagtccggga gtggggcgcc ccccctcctg cggcgactcc
     2821 gccggaccct cccgccgcgg tgcggagctg gccgcaggcg gcacgaatgt cggcgcccct
     2881 ccgctcccgg gtgatcgtct ggacccctcc cccaacgaga acgttccgga accggtccac
     2941 cacttccgcc accggcgccc ggtacggcga cgcttcgtgc gggttgtagg gaatgaggtt
     3001 gaccttgatc cgcgcgccac ggaacaggcg cgagagcgca agcgcgtcct cgggcgaatc
     3061 gttcacaccc gccagcagga cgtactccgc ggtcaccttc cgaccgcttt gcagcgggat
     3121 ccgccgcatc gcggcgacga cctccttcag cggatatttc cggttgatcg gcatcaggag
     3181 cgaacggaga tcgtcccggg cggcgttgat cgagacggcg aagcttacgg ggtacatcag
     3241 cgcgagcggc agcatctccg ggacgatccc cgccgtggag acggtgaccc gcttgcccga
     3301 gatcccgaat ccgaactgcg acaggaggat cccgatcgtc cgcgatactt ccggtacgtt
     3361 cagcagcggc tcccccatcc ccatgaagac gacgttcgac aggcgctcct cccgttccgc
     3421 gagtcgcttc gcggcgaaac aggcctgctg gacgatctcg gcggaggtca tgttccttcg
     3481 gatgccggcc gcgccggtgg cgcagaagcc gcacaggagc gggcacccca cctgggagga
     3541 gatgcacaac gtccgccgtc cctcgtccgg gatcagcacg ctttcgaccg cctcgccgtc
     3601 ctcgagccgg aagaggtact tctccgtccc gtcagcggag gtctcgacgc tctccgccgg
     3661 gaagcccgaa atgcggcacg acgctgccag gattccccgc agctccttcg acaggtccgt
     3721 catcgcagca aagtcgccgg catgtttctg gtagatccag cggaagagct gtcgggcgcg
     3781 gtaccgctcc ttcccccacc gggcgagaaa cccctccagc tcggggaggg tcatcccctt
     3841 caggtctgtg cgttccgcgt ccgtgctgat catattatta ccatatcacg gctcccgcgc
     3901 agtcccgttc gccggccggt ctgcgcctca cggggtaccc cttgcggggg acgccttgcc
     3961 cgtccatccg tggacgagcg gcggctccgt cgcgtctcgc tccgacatcc ccgtctcccg
     4021 cttcgacgct caggcccccg ccggggcacc ccttctcggc gcttgaccgg ccttcgtgct
     4081 gcgtttcgca aattcggcaa catacccgga ggcgccgcgg agcgggatac cccttccgag
     4141 cgggcatgcc atcgccgatg gccatggatg gccaagtgcc gcgggcgcaa ggatgcgcag
     4201 gagcggccag cgcagggcgc cagggatggc ggacggagcg gtagcgaggg agggagtatc
     4261 acgcggagag tgaggc
//
LOCUS       DASVJL010000311         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1834909, whole genome shotgun sequence.
ACCESSION   DASVJL010000311 DASVJL010000000
VERSION     DASVJL010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834909"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VF357_06595"
     CDS             complement(<1..1298)
                     /locus_tag="VF357_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7523852.1"
                     /translation="MALLSLRDIRVAFGGPSLLEGASLQVEPGDRICLLGRNGTGKST
                     LLKVVNGEIAPDEGEIVRQQGVKVALVPQEVPPGLSGTVYEVVSGGPRMAAEKAISRL
                     GLSPDADFGTLSGGKQRRVLIARALARESDILLLDEPTNHLDIDAIAWLENFLLREAR
                     TLLFVTHDRMFLRKLATRIVELDRGRLRDWACDYDTYLERREADLTAESGQRARFDKK
                     LAEEETWIRRGVKARGTRNEGRVRALERMREERRERREQVGTVRMQAQRAVPSGRLVV
                     EARGVEVGYGDRPVVRNFSTTILRGDRVGVIGPNGSGKTTLLRVLLGELSPRIGTVRL
                     GTRLTVAYSDQLREGFDEERTVAENLGDGSNTVTVNGRSRHVIGYLQDLLFTPDRARS
                     PVRVLSGGERNRLQLAKLFTKPFNLLVLDEPTNDLDIETL"
     gene            1447..2697
                     /locus_tag="VF357_06600"
     CDS             1447..2697
                     /locus_tag="VF357_06600"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX7523853.1"
                     /translation="MTSMKTHLPQGSVGFVETKRFTFAAPPHEMPMDIGEKLGPITLA
                     YETYGKLNREKSNAVLVQHAFSGSAHAAGFLPVQDPSKEKPGWWDFLIGPGRALDTEK
                     YFVICSNVIGSCYGSTGPSSLDPKTGRPYGLSFPVVTIADMVRAQAHLLDHLGIERLL
                     TVIGGSMGGMQALQWAVDYPDRAASAIPIATTSRHSPQQIAFNHVGRAAILADPEFHE
                     GDYYGWRTVPERGLSVARMVGFITYLSDRKMHEKFGRMKQLAAAKVSGRQGKWAEGTI
                     GQHSAVEFSVEGYLKHQGDIFVFDRRFDANSYLCITKAIDIFDLSGSSGRLAKAFENV
                     KSKFLIISFDTDWLYPTYQSQEIRNAILQNGLAVACLELSTIHGHDAFLIENEKPPEG
                     GKPGVKNKMTQVVRDFLGNVAARP"
     gene            2724..>3197
                     /locus_tag="VF357_06605"
     CDS             2724..>3197
                     /locus_tag="VF357_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523854.1"
                     /translation="MPKKIVLSFLIVLLAAGLSACSVMQGTYQKKVDEADTLTKRLAA
                     LQKKHDDLAAENAGLKADLAGMTLQNDKLTTDLAYVTGQRDKTAEDKEELDRILQSKS
                     DSLSQTIFDLRGKVADLDAENAKLKAENASLVKAREEQVRKVSSTYEDLLEKMKSE"
BASE COUNT          584 a         1067 c         1021 g          525 t
ORIGIN      
        1 tccagggtct cgatgtccag gtcgttcgtc ggttcgtcga ggaccaggag gttgaacggt
       61 ttcgtgaaga gcttcgccag ctgcaggcgg ttccgttccc ccccggagag gacccgcacg
      121 gggcttctgg cccggtcggg agtgaagagg aggtcctgga ggtagccgat cacgtgccgc
      181 gaccggccgt tgacggtgac ggtgttgctc ccgtctccga ggttttccgc cacggtcctc
      241 tcctcgtcga agccctcgcg aagctggtcg gaatacgcaa ccgtcaggcg ggttccgagc
      301 cgcaccgttc cgatccgcgg tgacaactct cccaggagaa cgcgcagcag ggtcgtcttg
      361 cccgacccgt tcggcccgat gacccccacc cggtcgcccc ggagaatcgt ggtcgagaag
      421 ttccggacca cgggacgatc gccgtagccg acctccaccc cccgggcctc gacgacgagc
      481 ctgccggagg gaacggcacg ttgcgcctgc atgcgcaccg tgcccacctg ctcccgccgc
      541 tcccgccgct cctcgcgcat cctctccagc gcgcgtaccc gcccctcgtt ccgggtgccc
      601 cgcgccttca cgccccggcg gatccacgtc tcctcctccg cgagcttctt gtcgaaacgc
      661 gcccgctgcc cgctttcggc cgtaaggtcc gcctcccggc gctcgaggta cgtgtcgtaa
      721 tcgcatgccc agtcgcggag tctcccgcgg tcgagctcca cgatccgcgt cgccagtttc
      781 cggaggaaca tccggtcgtg cgtgacgaag aggagcgtcc gcgcctcccg gagcaggaag
      841 ttctcgagcc aggcgatggc gtcgatgtcg aggtggttgg taggctcgtc gagaaggagg
      901 atgtccgact cccgggcaag cgcccgggcg atcagcaccc ggcgttgctt cccccccgaa
      961 agggttccga aatcggcgtc gggagatagt cccaggcggg agatcgcctt ctccgccgcc
     1021 atccggggac cgccggaaac gacttcgtac acggtccccg aaagaccggg cgggacctcc
     1081 tgggggacga gggcgacctt gaccccctgc tgacgaacga tctcgccttc gtccggcgcg
     1141 atctctccat tcacgacctt cagcagggtg gatttccccg tcccgttccg gcccaggagg
     1201 cagatccgat cccccggctc gacctgcagg ctcgccccct cgaggagaga cggtccgccg
     1261 aaggcaaccc ggatgtccct caagctcaac agcgccatgc tgcgtgctcc atttcatgat
     1321 ttgccccacg gtctctcccc gcggggcaag aagagagatc ggtttaccgc attgtgccac
     1381 attcccctgg agaaagcggt tccgcgggat ctcccgatcg cccgtccggc gggatctttt
     1441 ggtatcatga cctccatgaa aacgcacctt ccgcaaggtt ccgtcggttt cgtggagacg
     1501 aagcggttca ccttcgccgc gccgccccac gagatgccca tggatatcgg ggaaaagctg
     1561 gggcccatca ctctcgccta cgagacgtac gggaaactca accgcgagaa gagcaacgcc
     1621 gtcctcgtgc agcacgcctt ctccgggagc gcccacgccg ccggatttct cccggtgcaa
     1681 gacccctcga aggagaaacc ggggtggtgg gacttcctga tcggcccggg ccgcgctctg
     1741 gacacggaga aatatttcgt catctgctcg aacgtgatcg gatcgtgcta cggcagcacg
     1801 gggccgtcct cgctcgaccc gaaaacgggg aggccatacg gtctgtcgtt ccccgtggtg
     1861 acgatcgccg acatggtgcg tgcgcaggcg catcttctcg atcacctcgg catcgagcgt
     1921 ctgttgacgg tcatcggcgg gtcgatgggg gggatgcagg cgctccagtg ggcggtcgac
     1981 tacccggacc gcgccgcgtc ggccatcccg atcgcgacga cctcgcgaca ctcgccgcag
     2041 cagatcgcct tcaaccacgt cggccgggcg gcgatcctcg ccgaccccga gttccacgag
     2101 ggcgactact acggatggag gaccgtcccg gaacgcggcc tgtccgtggc gcggatggtg
     2161 gggttcatca cttacctctc ggaccggaag atgcacgaga agttcggccg gatgaagcag
     2221 ctcgccgcgg cgaaggtaag cgggcggcag gggaagtggg ccgaggggac gatcgggcag
     2281 cactccgccg tcgaattctc ggtggaaggg tacctgaagc atcaggggga tatcttcgtc
     2341 ttcgaccgcc ggttcgacgc caactcgtac ctgtgcatca cgaaggcgat cgacatcttc
     2401 gacctctccg gatcctcggg acggttggca aaagcgttcg agaacgtgaa gtcgaagttc
     2461 ctgatcatct ccttcgacac cgactggctc tacccgacgt accagtcgca ggagatccgc
     2521 aacgcgatcc ttcagaacgg actcgccgtg gcgtgcctcg agctctcgac gatccacggg
     2581 cacgacgcgt tcctcatcga aaacgagaag ccccccgagg gggggaaacc cggggtgaag
     2641 aacaagatga cccaggtggt gcgggatttc ctcggtaatg tcgcggcgcg gccgtgatgg
     2701 tcgaagcgaa aaggagggtc gtgatgccga aaaaaatcgt cctttcgttc ctgatcgttc
     2761 tgctggcggc aggcctgtcc gcctgctcgg tgatgcaggg cacctaccag aaaaaagtgg
     2821 acgaggcgga cacgctcacg aagcgcctcg ccgcgctgca gaagaaacac gacgacctcg
     2881 cggcggagaa cgccggtctc aaggccgacc tggcggggat gacgctccag aacgacaagc
     2941 tgaccaccga cctcgcctac gtcaccggcc agcgggacaa gaccgccgag gacaaggagg
     3001 agctcgacag gatcctgcag tcgaaatccg acagcctctc gcagacgatc ttcgacctgc
     3061 gcgggaaggt cgccgacctc gacgcggaaa acgccaagct caaggcggag aacgcgagcc
     3121 tggtgaaggc cagggaggag caggtccgga aagtgagctc gacctacgag gacctgctcg
     3181 aaaagatgaa gtcggag
//
LOCUS       DASVJL010000312         3791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_145981, whole genome shotgun sequence.
ACCESSION   DASVJL010000312 DASVJL010000000
VERSION     DASVJL010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145981"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VF357_06610"
     CDS             complement(<1..499)
                     /locus_tag="VF357_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7523855.1"
                     /translation="MKSPTVLIVDSNPRDLAKYSAVLTESRYTVLQAAECPEAVGILA
                     KHRGKLVVLSDLRVGEACGLDFLDDTLRKYPFLTFTFLASEPPLDSVIEALKRGAYDF
                     LRKPVAPDILLHSVARSVQKLSLTLETEKQEKEIRKLFERSREDLKDARTLSSFKGFM
                     ISMAAH"
     gene            complement(577..653)
                     /locus_tag="VF357_06615"
     tRNA            complement(577..653)
                     /locus_tag="VF357_06615"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(617..619),aa:Met,seq:cat)
     gene            complement(744..2246)
                     /locus_tag="VF357_06620"
     CDS             complement(744..2246)
                     /locus_tag="VF357_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX7523856.1"
                     /translation="MTPRPRNTALLLLVALLCLAAPAFAARQTLALFPPEIVPAGTDN
                     ALRPAVTLLEQTLKEKLADRFDVRPAGEGTAPAADDARRNKARTLGASYVFTGHLSRI
                     GKTVTLDVTIAPVEEPGKGRTIVVSGALENPSALTPRDLAMFRSLGTEAALKSKYVFF
                     GDERVGEGASAMSIPKLSGTISRGAPLPGEMMSTALSDTDLDGKMEFVAAYPDAIDVY
                     RVEGDELREKARIANAGPGLFHVDAADVTRNGVADIIAVRYKDGKALSDIWQFDGKEY
                     RKISSDLPYFLRTADLGPEGIVLLGQESDPGTIFKGPVFRIAVHRDGKTEVIGRDRPL
                     PLPEGTFLYGFTSLRKGKGVRYAALTDRNRILYLDETGKELWVGLDAVTGTEITLEGT
                     ERRLQIPGRMAAVDLNHDGTDELVVLNDLVAAGTYFESLRIFAQAEILCFAQGDTGLQ
                     LAWRSQQLDASARDLLADRSVPAKIRFAVASRDRAKLLVGAGQWRVLWVK"
     gene            complement(2243..2809)
                     /locus_tag="VF357_06625"
     CDS             complement(2243..2809)
                     /locus_tag="VF357_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX7523857.1"
                     /translation="MKVTRVLNREDAVLVVVDVQERLVPAIDKELYARSLKNFKITIE
                     AAGTLGLPIVLTEQYPRGLGHTVPDVLRALEGKTYARIEKVAFSCSRDEGFLAAIAKT
                     VRRQVILIGMETHVCVYQTSVDLINAGYEVFVLDDAVSSRFLHNYQSGIAALRDAGAV
                     VVSTETAVFQMMKVAGTPEFKKISSLLR"
     gene            complement(2819..>3791)
                     /locus_tag="VF357_06630"
     CDS             complement(2819..>3791)
                     /locus_tag="VF357_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX7523858.1"
                     /translation="SNVRRIASPKEVTAYYAEKSVTFPSIGFELDVLPVGTFLRFQQL
                     FPGAKSEDVSPIVREIRAAKSPHEAKALRENGKKLAALLFGARKKIRPGVTELALQGM
                     LQGEAIGGGHSTINRMRAFNQDPGLGCVISGPDAAVPSYADFPTAGKGLSPYVPAGQG
                     ERAIEPNEPVIIDLMWAQDGYLVDMARTYSVGPMHAKMEEAYIHAVAVLRAIEAGIRP
                     GAVAGDLYAAGLAVAAATPYARNFMGPPGYNTKFIGHGIGIEVDEFPFVANGVPTVLA
                     PGMVFALEPKFVFPGEGAVGLENTYLVTADGFETLTPLDESVLRCDG"
BASE COUNT          638 a         1194 c         1284 g          675 t
ORIGIN      
        1 cgtgcgcggc catcgagatc atgaacccct tgaacgacga gagggttcgc gcgtctttca
       61 ggtcctcccg actgcgctcg aagagtttgc ggatctcctt ttcctgcttt tccgtctcga
      121 gcgtcaggga gagcttctgc accgaccgcg cgacggagtg caggaggatg tcgggcgcga
      181 cgggcttgcg gaggaagtcg tatgcccctc gtttcaacgc ttcgatcacc gaatcgagcg
      241 gaggctcgga ggcaagaaag gtgaacgtca ggaaggggta tttccgcaag gtgtcatcga
      301 gaaaatcgag accgcacgct tcgcccacgc ggaggtccga caggacgacg agctttcccc
      361 gatgcttggc gaggatcccg accgcttccg ggcactctgc tgcctgcaga acggtgtagc
      421 gggactccgt caggaccgcg gaatacttcg ccagatcccg cgggttcgaa tccacgatga
      481 ggacggtagg ggatttcatg gatggagcat tctaccacgg cggtgcaggg aatggacgcc
      541 ggttccactc ccccgtagac gccaatcggt gaagagtggt ggcggtgcag ggaattgaac
      601 cccggacact gcggatatga gccgcatgct ctaaccatct gagctacacc gccacgctcg
      661 gtcgaaccgg gaattatacg tcaggagggg atattgtcaa ttccgggcgt ggattccttg
      721 ggcggatgcc cctctgcgga acctcacttg acccagagga cgcgccactg gccggcgccg
      781 accaggagct ttgcgcggtc gcgcgaggcg acggcgaagc ggatcttggc cgggacggac
      841 cggtcggcga ggaggtcgcg ggcggaggcg tcgagctgct gcgagcgcca ggcgagctgc
      901 aggccggtgt ccccttgcgc gaagcagaga atctcggcct gggcgaagat ccgcaggctc
      961 tcgaaatacg ttcccgcggc caccagatcg ttcaggacga ccagctcgtc ggtgccgtcg
     1021 tggttgaggt cgaccgccgc catccgtccg gggatctgca gccgccgctc agtcccctcg
     1081 agggtgatct cggtcccggt gacggcgtcc agcccgaccc acagctcttt cccggtctcg
     1141 tcgaggtaaa ggatccggtt gcggtcggtc agcgcggcgt accggactcc cttccccttg
     1201 cgcagggagg tgaatccgta caggaacgtc ccctccggaa gcggcaaggg gcggtcgcga
     1261 ccgatcacct cggtcttccc atcccggtgg acggcgatcc ggaagaccgg ccccttgaaa
     1321 atcgtccctg gatcagactc ctgcccgagg aggacgatcc cttccgggcc gaggtcggcc
     1381 gtgcgcagga agtatggcag gtccgatgag atcttccggt actccttccc gtcgaactgc
     1441 cagatgtcgg agagcgcctt tccgtccttg taccggacgg cgatgatgtc ggccacccca
     1501 ttgcgggtga cgtcggcggc atccacgtgg aagagccccg ggccggcgtt ggcgatccgg
     1561 gccttttcgc ggagctcgtc cccctccacg cggtagacat cgatcgcgtc ggggtaggcg
     1621 gcgacgaact ccatcttccc gtcgaggtcg gtgtcggaga gtgcggtgga catcatttcg
     1681 ccagggagcg gggcgccgcg gctgatcgtc ccggaaagct tggggatgct catcgcggag
     1741 gcgccctccc ccacccgctc gtcgccgaag aagacgtatt tcgacttgag tgccgcttcg
     1801 gtgcccaggc tgcggaacat ggcgagatcg cgcggggtga gcgcggacgg gttctcgagc
     1861 gcgcccgaga cgacgatcgt gcgccccttc cccggttcct ccacgggggc gatcgtcacg
     1921 tcgagcgtca ccgtttttcc gatgcgcgag aggtgcccgg tgaagacgta ggaggcgccg
     1981 agcgtcctgg ccttgttccg gcgtgcgtcg tcggcggccg gggcggttcc ctcacccgca
     2041 gggcgaacgt cgaagcggtc ggcgagcttc tccttcaacg tctgttcgag gagcgtgacg
     2101 gcggggcgca gcgcattgtc ggtccccgcg gggacgattt ccggcggaaa gagggcgagg
     2161 gtctggcgtg ccgcaaaggc gggcgcggca aggcataaaa gggccacaag gaggaggagc
     2221 gcggtattcc gcgggcgggg ggtcatcgaa gcagcgacga gatcttcttg aactccggcg
     2281 tcccggcgac tttcatcatc tggaagacgg ccgtttcggt gctgacgacc acggcccccg
     2341 cgtcgcgcag ggccgcgatc ccgctctgat agttgtggag gaagcgcgag gagacggcgt
     2401 cgtccagcac gaacacttcg tacccggcat tgatgaggtc gacggaggtc tggtagacgc
     2461 agacgtgggt ttccatgccg atcaggatca cctgacgacg gacggtcttc gcgatggcgg
     2521 cgaggaaccc ttcgtcgcgg gagcagctga aggcgacttt ctcgatccgc gcgtacgtct
     2581 tcccctcgag cgcacgcaag acgtcgggga cggtgtggcc cagccccctg gggtactgtt
     2641 ccgtgaggac gatcggaagg cccagcgtcc cggccgcctc gatggtgatc ttgaagttct
     2701 tgagcgaccg cgcgtacaac tccttgtcga tggcggggac caggcgttcc tgcacgtcga
     2761 ccacgaccag cacggcgtct tccctgttca ggaccctggt cactttcatg gctctccttc
     2821 acccgtcgca gcggaggacg ctctcatcga gcggcgtcag cgtctcgaac ccgtccgcgg
     2881 tcaccaggta cgtattctca agccccacgg caccctcccc cgggaagacg aacttcggct
     2941 cgagggcgaa caccatgccg ggcgcaagga ccgtgggaac cccattcgct acgaagggaa
     3001 actcgtccac ctcgatcccg atcccgtgac cgatgaattt tgtattatac cccggcgggc
     3061 ccatgaaatt ccgagcgtaa ggtgtcgcgg cggcaacggc aagcccggcg gcgtacaagt
     3121 cccctgctac ggcgccggga cggatccccg cttcgatcgc tcgcagcacg gcgacggcgt
     3181 gaatgtatgc ctcctccatc ttcgcgtgca tcgggccgac gctgtacgtg cgggccatgt
     3241 ccaccagata gccgtcctgc gcccacatca ggtcgatgat gaccggctcg ttcggctcga
     3301 tcgcgcgctc cccctgcccc gccgggacgt aaggtgagag gcccttcccg gccgtgggaa
     3361 agtcggcgta agagggaacg gccgcgtcgg ggcccgaaat tacgcacccg aggcccggat
     3421 cctggttgaa ggcacgcatc cggttgatgg tgctgtgccc gccgccgatc gcctccccct
     3481 gtagcatccc ctggagggcc aattccgtga cgccggggcg gatctttttc ctggcgccga
     3541 agagcagcgc ggcgagcttt ttcccgtttt cccggagcgc ctttgcttcg tggggcgatt
     3601 tcgctgcgcg gatctcccgg acgatcgggg agacgtcctc ggactttgcg ccggggaaga
     3661 gttgctggaa acggaggaag gtccccacgg gaaggacgtc aagttcgaat ccgatggagg
     3721 ggaaggtgac gctcttttca gcgtagtagg cggttacctc tttcgggctc gcgatccggc
     3781 gcacgttgga a
//
LOCUS       DASVJL010000313         5901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_613662, whole genome shotgun sequence.
ACCESSION   DASVJL010000313 DASVJL010000000
VERSION     DASVJL010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613662"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VF357_06635"
     CDS             <1..741
                     /locus_tag="VF357_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7523859.1"
                     /translation="HPDAGALYWAYAESKVIVLDLGTKEAPKEVPPKYYMKKPWSEIK
                     AFRSTSCIVGALLGREKPIGIFAIDKKLKKLRVTEDDISLVNLLQDIASYAIQNIQTV
                     NELKVHQSELYDLIRITIDQATNGKEKAGRMTEVNRGLMDSSKKIAGITATIGDIADR
                     TNLLSLNAAIEAARAGEAGRGFGVVADEVKKLAGQTHDSTQEIGDIVGRISAEIQNSS
                     GTMNDIVVAQQKLIDSIEILNSKALSLV"
     gene            1021..2448
                     /locus_tag="VF357_06640"
     CDS             1021..2448
                     /locus_tag="VF357_06640"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7523860.1"
                     /translation="MYSILVVDDEPAIRRLLADFFGSHGFEIVGVGDGQAGIDLLRQR
                     KFDLLFLDLTMPGMGGIDVLREVKVLDIHIHSIVITGFGTIQSAVEAVRLGAYDYITK
                     PFNLDELMITVNRVFDLMKLQKENTVLKKQIKEKYNFKKLIGNSRRMQVLHRFIEKIS
                     DTDSTVLITGESGTGKELVAKTIHFNSSRVSEPFVPLNCGAIPRDLIESELFGHEKGA
                     FTGAINSRVGRFELANGGTIFLDEIGELPFPLQVKLLRVVQEREFERVGGTRTIKVDV
                     RILAATNRDLEKAVAEHTFREDLFYRLNVIPVDIPPLREREDDVLLLVNHFIGEFCRR
                     KRRPLCKVSREASDLLCNYHWPGNVREVENAVERMIILNETGEIGVEDLPLRIVASRG
                     NVGKLQPVPAVAAPADVPSYVPWTESGVDLNGILDEIEKKLIVQALKQSGGVKNKAAT
                     LLGLNRTTFLEKVKKMGVSAATNGS"
     gene            2582..4363
                     /locus_tag="VF357_06645"
     CDS             2582..4363
                     /locus_tag="VF357_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7523861.1"
                     /translation="MARILAYLLLLMLPAVSFAGVADGLVPAGSLSNALALVEANRIS
                     DAMKTLATFRPDMATLGPYHYIYGRAFASARNPHKAASSYRRASIYAANADLKELSLF
                     LAAETELGMGYNYEAKADCLIFLKKFPNSARVRRVRIVLARSLFMTGRLSEAFRQFDL
                     AGNTPEALYGKADTFQKMRMTTEASQAYAVAAAADARYPDSSEESRYWLGENLRLSGD
                     AGHAKELLLTIMGEGLRDRAALGLGEMAAAASQPDEAIRRFVSVLGAKDKKVRRLALL
                     RLADVEAVQGNAGKAAARLTEIVQMYPFTREYDDAVLRLAGLKATAGDEIGALSLLTK
                     LVLRASPLRKNALDGIEAILLSARGKGGERFVALWNAGGRWLMDASRQASLVTIAGEL
                     KGTGAPYLELVRWLSQYGSGAVRVENLVALSRLCVEAGDVAGVRNCLKSLKGMGASGD
                     EVLRVEAALKFAQKDYRGAAETLSMLRSVEGKDAFLLGETLPYAGSSRTAAAALETAV
                     SRSDAGSRELSRLADALVDAGRKDDAARYYRGAVEKDPTNEWACYRLALLLGKNGGEE
                     YLKKIKTDPTLARMARAARREMTLDAE"
     gene            4353..5549
                     /locus_tag="VF357_06650"
     CDS             4353..5549
                     /locus_tag="VF357_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7523862.1"
                     /translation="MQNNSGSPAERIRHLSEAFSSFNVASGALEQYYEKLQETVRHLT
                     IELRERNAQLKTALSEAETAKDNLRCVLQSMDEVVVVLDPDGNMTMANRAAAEMLGPA
                     VADGAGKPLESLGFTLGKCGADAVLVANGKRYDVIASRSNVVDPSGMVRGSVLLVRDV
                     TKMKELESQFERNRRLIRMGEMAAKIVHEIRNPLCSIELYATMLESGLGKAEEAKLAR
                     GISSGIRSLDNILTNMLLFAKQQKPSLTRVDTAGIVDEALFLLEPMIGSREVRIEKCV
                     AGAPCLDGDPELLKQVLLNVLLNAIHATPAEGRIKVSAREEDDAAVIEVSDEGEGIRE
                     EDLERIFDPFFSTKVKGTGLGLAITARIMEAHGGFIRVRSEVGRGSVFELHFPPPGTL
                     SEGAVT"
     gene            5546..>5901
                     /locus_tag="VF357_06655"
     CDS             5546..>5901
                     /locus_tag="VF357_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7523863.1"
                     /translation="MKPILVVDDDPQMRSALKEAIQRIGYGADVCENGQEAIGRISRI
                     DYSLVVTDMKMPGMDGLSLLREIHRRCATQPVVVITGYATVENAVETMREGATDYLLK
                     PFSFDALKKAVLSVMAR"
BASE COUNT         1186 a         1805 c         1889 g         1021 t
ORIGIN      
        1 cacccggacg ccggcgcgct gtactgggcc tacgccgaaa gcaaggtcat cgtcctcgac
       61 ctgggaacca aggaggctcc gaaggaagtt ccgccgaagt attacatgaa gaagccgtgg
      121 tcggagatca aggcgttccg atccacgtcg tgcatcgtcg gcgcgctcct ggggagggag
      181 aaaccgatcg gaattttcgc gatcgacaag aagctcaaga agctccgcgt gaccgaggac
      241 gacatcagtc ttgtcaacct gctccaggac atcgcatcct atgccatcca gaacatccag
      301 accgtaaatg aactgaaggt ccaccagagc gaactctacg atctcatccg catcacgatc
      361 gatcaggcga cgaacggcaa ggagaaagcc ggccggatga ccgaggtcaa caggggcctg
      421 atggattctt ccaagaagat cgccgggatc accgcgacga tcggcgacat cgcggaccgg
      481 acgaacctcc tgtccctcaa cgccgccatc gaggcggcgc gggcgggaga ggccgggcgc
      541 ggcttcggcg tggtggcgga cgaggtcaag aagctcgccg gacagacgca cgactcgacg
      601 caggagatcg gcgatatcgt cggacggatc tcggcggaga tccagaactc cagcgggacg
      661 atgaacgaca tcgtcgtcgc ccagcaaaaa ttgatcgact ccatcgagat cctgaacagc
      721 aaggcgctga gcctcgtctg agccccttgg cgcgagccag gctccccgtt tcccaccgga
      781 ccttcccttt ccccgtcgcg ccagcacggt ccattgactg agagtaatac ctgataatgc
      841 aggattacgc ccaaaccccg ccaaaatgtg gccaatagat caatccgttc acgacatcat
      901 gtcaaagttg cgtctagcga tacgatactt agtgaattat gcgtcttcat tatccgatat
      961 agttaaagat ggatcgttcc ggtcccattg ggataatgat attcgtcgag aggatcaagg
     1021 atgtattcca tactcgttgt tgacgacgaa ccggccatcc ggagattgct cgccgatttt
     1081 ttcggctcgc acggttttga gatcgtcggg gttggggacg gtcaagccgg aatcgatctg
     1141 ctcaggcaac ggaaattcga cctcctcttc ctcgatctca cgatgcccgg catggggggg
     1201 atcgacgtcc tgcgggaggt gaaggtcctc gatatccaca tccactcgat cgtcatcacg
     1261 gggttcggca cgatccaatc ggccgtggag gcggtgcggc ttggcgcgta cgactacatc
     1321 acgaagccgt tcaacctcga cgagctcatg atcacggtca acagggtatt cgacctcatg
     1381 aagttgcaga aggagaacac ggtcctcaag aagcagatca aggaaaagta caacttcaag
     1441 aaactgatcg ggaattcccg gcggatgcag gtcctccacc gtttcatcga gaagatctcc
     1501 gacaccgaca gcaccgtcct catcacgggc gaaagcggaa cggggaagga actcgtcgcg
     1561 aagacgatcc acttcaacag ctcccgcgtg agcgaaccgt tcgtaccgct caactgcggg
     1621 gcgatccccc gggacctgat cgagtccgag ctgttcgggc atgagaaggg cgccttcacg
     1681 ggcgccatca actcgcgcgt cggccgcttc gaactcgcca atggcgggac cattttcctc
     1741 gatgagatcg gggaactgcc cttccctctg caggtcaagc tgctccgggt ggtacaggag
     1801 agggagttcg aacgggtcgg cggcacgcgc acgatcaagg tcgacgtccg gattctcgcc
     1861 gcgacgaacc gcgatctcga gaaagccgtg gccgagcaca cgttccggga ggacctcttc
     1921 taccgcctga acgtcattcc ggtcgatatt ccgccgctcc gggagcggga agacgacgtc
     1981 ctccttctcg tcaaccactt tatcggggag ttctgccgca ggaagaggag gcccctctgc
     2041 aaggtttccc gcgaggccag cgatcttctt tgcaactacc actggccggg caacgttcgc
     2101 gaagtcgaga atgcggtaga gcggatgatc atcctcaatg agaccgggga gatcggcgtc
     2161 gaggatctcc cgctgcgcat cgtggcgagc cggggcaacg tcggaaaact ccagccggtt
     2221 cccgccgtcg cggccccggc ggatgtcccc tcctatgttc cgtggacgga gtcgggggtc
     2281 gacctgaacg gcatcctcga tgagattgag aagaaactca tcgtccaggc tctaaagcag
     2341 tccggcgggg tgaagaacaa ggctgcgacc ctgttggggc tcaaccggac gaccttcctt
     2401 gaaaaagtca agaaaatggg ggtgtcggcg gcaacgaacg gatcctgatc gccggcccct
     2461 gctcctgtca tttcttcggt gcccgggcgc gtgtcccgtc agttctctga cacggccata
     2521 gaaataaact cctttaaaat cagcttgttg ctgtggcaca ctccttgcaa acataaggga
     2581 catggcacgg attcttgctt acctcctgct cctgatgcta ccggccgttt ccttcgcggg
     2641 ggtggccgat ggcctggttc ccgcgggctc cctctcgaat gcacttgccc tcgtggaggc
     2701 gaaccgcatc tccgacgcga tgaaaaccct cgcgaccttc cggccggaca tggcaacact
     2761 cgggccgtat cactatatct acgggcgggc gtttgcctcc gcccggaacc cgcataaagc
     2821 cgcctcgagc taccggcgcg cctcgattta cgcggccaac gccgacctga aggaactgtc
     2881 gctcttcctt gcggccgaaa ccgagctcgg gatgggctat aactacgagg cgaaggcgga
     2941 ctgccttatc ttccttaaga aattcccaaa ctccgcgcgc gttcggcggg tacggatcgt
     3001 gctcgccagg agtctcttca tgaccggccg cctctccgag gcgttccgcc aattcgacct
     3061 ggccgggaat acgcccgagg cgctttacgg gaaagcggac accttccaga aaatgaggat
     3121 gaccacggaa gcgtcgcagg cgtatgccgt cgccgccgcc gctgacgcgc gttatcccga
     3181 ttcttccgag gagagccgct actggcttgg cgagaatctc cgtctgtcgg gcgacgcggg
     3241 gcatgcgaag gagcttctgc ttacgataat gggggagggg cttcgagatc gcgcggcgct
     3301 cggtctcggt gagatggctg ccgccgcctc gcaaccggat gaagcgatcc ggcggttcgt
     3361 ctcggtgctt ggggcgaagg ataagaaggt gcgtcgcctc gccctgctcc gcctggcgga
     3421 cgtcgaggcg gtccagggga acgccggaaa ggccgccgcg cggctcaccg agatcgttca
     3481 gatgtatccg ttcacccggg aatacgacga tgccgtcctt cggctcgccg gcctgaaggc
     3541 gacggcgggt gacgagatcg gcgccctttc ccttctcacg aaactggtcc tgcgcgcttc
     3601 gccgcttcgc aagaatgccc tcgacgggat cgaagcgatc cttctttcgg cacgggggaa
     3661 ggggggggag cggtttgtcg ctctctggaa cgccgggggg cgctggctga tggacgcgtc
     3721 gcggcaggcc tccctcgtca cgatcgccgg ggagctcaaa gggacgggcg ctccgtacct
     3781 cgagcttgtc cggtggctgt cgcagtacgg gtcgggcgcc gtccgcgtgg agaatctcgt
     3841 cgccctttcc cgtctatgcg tcgaggccgg tgacgtggcc ggggtgcgga attgcctgaa
     3901 aagcctcaaa gggatggggg catccggaga tgaagtcctt cgggtcgagg ccgccctgaa
     3961 gttcgcgcaa aaagattacc ggggagctgc agaaacgctc tcgatgttgc ggagcgtcga
     4021 gggaaaggac gctttccttc tgggggaaac gctcccgtac gccgggagct cccgaacggc
     4081 cgcggcggca ctggaaacgg cggtgtcccg gtccgacgca ggctcgcgcg agttgtcgcg
     4141 cctggccgac gcgctggtcg acgcgggacg caaggacgac gcggccagat attaccgggg
     4201 ggccgtcgag aaagacccga cgaacgaatg ggcgtgctac cggctggccc ttctcctggg
     4261 gaagaacggc ggcgaggagt acctgaaaaa gatcaagacg gacccgacgc tcgcgcggat
     4321 ggcgcgggcg gcgaggaggg agatgaccct ggatgcagaa taacagcgga agccctgcgg
     4381 aacgaatccg gcacctcagc gaggcgttct cctcgttcaa tgtcgcgtcg ggcgccctcg
     4441 agcaatatta cgaaaagctc caggagacgg tgcgccacct cacgatcgag ctcagggagc
     4501 gaaacgccca gctcaagacc gctctctcgg aggcggaaac ggcgaaagac aacctccggt
     4561 gcgtccttca gagcatggat gaggtggtcg tcgttctcga ccccgacggg aacatgacga
     4621 tggcaaaccg ggccgcggcc gaaatgctgg gccccgccgt ggccgacggc gccggcaagc
     4681 cgctcgagag tctcggattc accctcggga aatgcggcgc cgacgccgtc ctcgtcgcga
     4741 acggaaagcg gtacgacgtc atcgcgtcgc gctcgaacgt cgtcgacccc tcgggcatgg
     4801 tgcgcgggag cgtcctcctc gtccgtgacg tcaccaagat gaaggaactc gagtcgcagt
     4861 tcgagcggaa ccgcaggctc atccggatgg gcgagatggc cgccaagatc gtacacgaga
     4921 tccgcaatcc cctctgcagt atcgagctct acgcgacgat gctggagagc gggcttggca
     4981 aggcggagga ggcgaaactc gcccggggaa tatcgagcgg catccggagc ctcgacaaca
     5041 tcctcacgaa catgctcctt ttcgcgaagc agcagaaacc gtctctgacc cgcgtcgaca
     5101 cggccgggat cgtcgacgag gccctcttcc tgctcgaacc gatgatcggc tcgcgggaag
     5161 tgcgcatcga gaaatgcgtc gcaggcgcgc cgtgcctcga cggtgacccg gagctcctca
     5221 agcaggttct gctgaacgtc ctcctcaacg ccatccacgc gacgcctgcg gagggccgaa
     5281 tcaaggtgtc cgcccgggag gaggacgatg ccgcggtcat cgaggtctcc gacgagggcg
     5341 aggggattcg cgaagaggac ctggaacgga tcttcgaccc gttcttcagc acgaaagtga
     5401 aggggaccgg gcttggcctc gcgatcaccg cgaggatcat ggaggcgcac ggcgggttca
     5461 tcagggtgcg gagcgaggtg ggcaggggga gcgtgttcga gcttcacttc cccccccccg
     5521 ggacgttgag cgagggggcg gtaacgtgaa gccgatcctc gtcgtggacg acgatcccca
     5581 gatgcggtcg gccctgaaag aggcgatcca gcggatcggc tacggcgccg acgtgtgcga
     5641 gaacggccag gaggccatcg gccggatctc ccgcatcgac tactcgctgg tcgtgaccga
     5701 catgaagatg ccggggatgg atggcctgtc cctcctccgg gagatccacc gccgctgcgc
     5761 gacccagccc gtcgtcgtca tcacgggcta cgcgaccgtg gagaacgccg tcgagacgat
     5821 gcgggaaggg gcgacggatt atctcctgaa gccgttctcc ttcgatgcgc tcaagaaggc
     5881 cgtcctgtcc gtgatggccc g
//
LOCUS       DASVJL010000314         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_897149, whole genome shotgun sequence.
ACCESSION   DASVJL010000314 DASVJL010000000
VERSION     DASVJL010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897149"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1880)
                     /locus_tag="VF357_06660"
     CDS             complement(<1..1880)
                     /locus_tag="VF357_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX7523864.1"
                     /translation="MSTGLPGLDQVFTGILPGDNIVWQVDVVEDYRPFVDPFVRDAIA
                     RGKRLVYFRFARHPELVPRGVGAEIHTLHPEVGFETFTARIHKVIEEAGRGAFYVFDC
                     LSDLAADWYSDLMLGNFFMVTCPYLFDLDTVTYFALLRDGHSFDAVSSIRGTTQLLID
                     VFRHKDRLYVHPLKVDRRTSPTMYLPHVRDGKAFRPLTESAVLSEVLEDLSKRRVDAV
                     SRTLDIWDRKLLQAREVIDEVRQGGRPGSEAEEIFGRLLRMMVTRDERLVALASRWFD
                     LDDLLSLRKRMIGTGLIGGKSVGMLLARAILCKADPRWRDRLETHDSFYIGSDVFYTF
                     LVRNGCWRARRGQRNADTFMDGADVARERILSGTFPGFLQEQFVAMLEYFGQSPIIVR
                     SSSLLEDSFGNAFTGKYDSVFRPNQGSPQERLSAFLEAIRSVYASTMSPEALLYRAHR
                     GLIDRDEQMAVLVQRVSGAVHGHLFYPQVAGVGLSYNPYVWSEQIDPEAGVLRLVFGL
                     GTRAVDRSDDDYTRLVALNAPQRRPDAERSGSPAFSQRRVDLLDLRANRFTSERIDDV
                     ARASSDLPIDLYAVRRSALPRGEGATPRPSDEGWVLTFGKLLSETPFVPYLREMLGIL
                     RDA"
     gene            complement(2026..>2338)
                     /locus_tag="VF357_06665"
     CDS             complement(2026..>2338)
                     /locus_tag="VF357_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX7523865.1"
                     /translation="KPIVEKYNVLQKFGYFLLFAVSVLQSLLGFALYTYLWPAQFAFV
                     YAVFGDAVQIRIWHTTIMWLFLSFTALHVYLVVTEDKRMIRAMVDGYYYRKTPGGAAD
                     G"
BASE COUNT          413 a          774 c          796 g          355 t
ORIGIN      
        1 gcgtcccgga ggattcccag catctcccgc aggtacggga cgaacggcgt ctccgacagg
       61 agtttcccga acgtgaggac ccacccttcg tcggacggcc ggggagtcgc tccctcgccg
      121 cgcggcaggg cgctccggcg cacggcgtag agatcgatcg gcaggtcgga ggaggcgcgc
      181 gccacgtcgt cgatcctctc cgacgtgaac cggttcgccc gcaggtcgag gaggtccacc
      241 cggcgctgtg agaaggccgg ggagccggat cgctccgcgt cggggcggcg ctgcggcgcg
      301 ttcagcgcca ccaggcgggt gtaatcgtcg tcggaccggt cgaccgcccg ggtccccaac
      361 ccgaagacca ggcgcaacac cccggcttct gggtcgatct gctcgctcca gacgtatggg
      421 ttgtacgaca ggcccacccc cgccacctgc ggatagaaca ggtgcccgtg gaccgccccc
      481 gacacgcgct ggacgaggac cgccatctgc tcgtcccggt cgatcagccc ccggtgcgcg
      541 cggtacagaa gcgcttcggg gctcatcgtg ctcgcgtaca cggaccggat cgcctcgagg
      601 aacgcggaga ggcgctcctg cggcgatccc tggttcgggc ggaagacgct gtcgtacttc
      661 cccgtgaagg cgttgccgaa actgtcctcg aggaggctgc tcgatcggac gatgatcggc
      721 gactggccga aatactcgag catcgccacg aactgctcct ggaggaagcc agggaaggtg
      781 cccgacagga tccgctccct cgccacatcc gccccgtcca tgaaggtgtc ggcgttgcgc
      841 tggccgcgcc gcgcccgcca gcatccgttg cggacgagga acgtgtagaa gacgtcggag
      901 ccgatgtaaa acgagtcgtg cgtctccagc cggtccctcc agcgagggtc cgccttgcag
      961 aggatcgcgc gggcgaggag catcccgacc gacttcccgc cgatcagtcc cgtgccgatc
     1021 atccgcttcc ggagggagag caggtcgtcg aggtcgaacc accgggaggc gagggccacc
     1081 aaccgctcgt cgcgggtgac catcatccgg agaagccgcc cgaagatctc ctccgcctcg
     1141 cttccggggc ggcccccctg gcgcacctcg tcgatcacct cccgcgcctg gaggagcttc
     1201 cggtcccaga tgtcgagcgt gcgcgaaacg gcgtccacgc gccgcttcga gaggtcctcc
     1261 agcacctcgg agagcaccgc gctctccgtg agggggcgga acgccttgcc gtcgcggacg
     1321 tgggggagat acatcgtcgg agaggtacgc cggtcgacct tcaacgggtg gacgtagagg
     1381 cgatccttgt ggcggaagac gtcgatcagc agctgcgtcg tgccgcggat cgacgagacg
     1441 gcgtcgaacg agtgtccgtc gcgcagaagg gcgaagtagg tcaccgtgtc gaggtcgaac
     1501 aggtacgggc aggtgaccat gaagaagttg cccagcatca ggtcgctgta ccagtcggcc
     1561 gcgaggtccg agaggcagtc gaagacgtag aaggcgccgc gccccgcctc ctcgatcacc
     1621 ttgtgaatcc gggcggtgaa cgtttcgaag cccacttcgg ggtggagggt gtggatctcc
     1681 gccccgactc cccgggggac gagctccggg tggcgggcga agcggaagta gacgagccgt
     1741 ttcccgcggg cgatcgcgtc gcggacgaac gggtcgacga aggggcggta atcctcgacg
     1801 acatccacct gccagacgat gttgtcgccc ggcaggatcc ccgtgaagac ctggtcgagg
     1861 ccgggcaacc cggtgctcaa cgtctccgat ttccggcgcg cggccagggc gtcacccccc
     1921 tacggatcga tcctttccga aggtacgtta ccatggaaaa gaatcggccc gcgacgggtg
     1981 gatatcccgc ggcgggccgc gatggccggg ctcgttctcc cccggttatc cgtccgccgc
     2041 cccccccggt gtcttccggt agtagtaccc gtccaccatc gcacggatca tccgcttgtc
     2101 ctcggtgacg accaggtaga cgtgcagcgc cgtgaaggag aggaacagcc acatgatcgt
     2161 ggtatgccag atgcggatct gcaccgcgtc tccaaagacc gcgtacacga aggcgaactg
     2221 cgccggccag agataggtgt aaagggcaaa tccgagcagg gactgaagga cggacaccgc
     2281 gaagagcagg aagtagccga acttctgcag cacgttgtac ttctcgacga tcggtttt
//
LOCUS       DASVJL010000315         3900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_708630, whole genome shotgun sequence.
ACCESSION   DASVJL010000315 DASVJL010000000
VERSION     DASVJL010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708630"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VF357_06670"
     CDS             complement(<1..209)
                     /locus_tag="VF357_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523866.1"
                     /translation="MPLRRSLHVVISALLILLLASCAGREVKPIPVEPPRKAVKVALV
                     LGAGAAKGFAHVGVLKVLEANHVPV"
     gene            complement(223..1014)
                     /locus_tag="VF357_06675"
     CDS             complement(223..1014)
                     /locus_tag="VF357_06675"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7523867.1"
                     /translation="MGKRNGMPVILAFGGFDPTGGAGVLMDSRAAAAAGVHASAVVSC
                     LTVQTTASFSRFASVPRDVLDESLAAAAKSFPLGAVKVGMVGTRAAAEAILSFVAAHR
                     DLPLVLDPVLRSSSGAPLLSSSALPAYRQLLRRAAVLTPNLPEAEKLLDRRIVSFADA
                     AEAAGELSALTGASVVLKGGHFPWRGRRGTDIVFSDGRTTLLPPIGARRGDPHGTGCA
                     LAAVIAARIAAGDAVVPAVLAAKSLLSKLVAGGFPSAEGRPTLFP"
     gene            complement(1020..2462)
                     /locus_tag="VF357_06680"
     CDS             complement(1020..2462)
                     /locus_tag="VF357_06680"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7523868.1"
                     /translation="MKRILIADDDESIRWVLRKTVTGMGFAADLAEDGEQALTLLGKN
                     TYSAAFVDIRMPGVEGIEVLERVQARKSPTLFFIMTAVHLPDVAARSTRAGAAEFITK
                     PFELSRIEELLRNVAQESSSRESSFRPEEREEWSSARIVGKSRAILEVFQKIGKVADS
                     DATILLLGERGVGKELIARCIHDLGRRTGPFVAVNIPAIPRDLQEAELFGHEKGAFTG
                     AESARPGKLSAAMDGTLFLDEIGDTPGGLQAKLLRFLQEREYAPVGSDQPRKFRGRII
                     AATNRNLQKMVSDGKFREDLFDRLNVFPLRVPSLAERKEDIPLLADYFLQKYCAVLSR
                     PPRSFSKEAVEELSARPWKGNVRELENFVQRLAVLSPGKLLRRDDVVRELTREKGAPE
                     TSSSPMEQQVEERIREFLRRLGPALDSETRLHELFVRQVECPLLKVVLEATAGNQIRA
                     AAILGIHRNTLRKKIAELGLAPKARKRKGP"
     gene            complement(2459..3580)
                     /locus_tag="VF357_06685"
     CDS             complement(2459..3580)
                     /locus_tag="VF357_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7523869.1"
                     /translation="MSDLLRQTLESVNIGILVFDVAGKLAYINPAAEEILQGSSQALA
                     GKHFRTLFRGSPEAIRIVRKAIEENTPVTGFDVALKPVGGGQTVQRRGAASIPVMLGA
                     SPLSGASGDPQGAVLSVKSSEILSLVGQEERATVRAEEMQMLAYGIAHEIKNPLGGIL
                     GAAQWILRGEGPDEDRAEGVRLILREARRINDLVEKMLEMGKSPPPPRPFALPPLLRE
                     AEQLLLSEARAQGKEVRFELRVDPSLPPISGHPDTVYRALLNILKNAVEAIERAGTVG
                     IEARMNVNYRFARGRGRKRSFLEVEITDNGKGMTEEELHKALLPFYTTKARGTGLGLV
                     MARQAVTRLGGKMEIRSSPGAGTAVLLSLPVDPRRKAAT"
     gene            complement(3577..>3900)
                     /locus_tag="VF357_06690"
     CDS             complement(3577..>3900)
                     /locus_tag="VF357_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX7523870.1"
                     /translation="GCDSVMIGRAAMGNPWIFAALKREIALPAGPAGTLPFAPFPTPE
                     ARRALILRHGEEMFLRHGRHGIKEMRKHLAWYSRGIQGAAAFRSELPKIADPDSFRAV
                     VERFF"
BASE COUNT          624 a         1309 c         1335 g          632 t
ORIGIN      
        1 tggacgggaa cgtggttcgc ttcgagcacc ttcagcactc ccacgtgggc gaaccccttg
       61 gccgccccgg cccccagcac gagggccacc ttcaccgcct tgcgcggcgg ctcgacgggg
      121 atcggcttca cttcgcgacc cgcgcatgag gcgagcagca ggatcaggag cgctgaaatg
      181 acgacgtgca gggagcgtcg caagggcatc gggaacctcc ggtcaaggga acaaggtggg
      241 gcgtccctcc gccgagggaa agccgccggc gaccagtttc gaaagaaggg atttcgcggc
      301 caggactgcg ggaacgacgg cgtcccccgc cgcgatccgc gcggcgatca ccgccgcaag
      361 ggcgcatccg gtgccgtgcg ggtcgccgcg acgggcgccg atcggcggaa ggagcgtggt
      421 ccggccgtcg gaaaagacga tgtccgttcc ccggcgtccc ctccatggga agtggccccc
      481 cttgaggacg acggaggcgc cggtcagcgc agagagttct cccgcggcct ccgccgcgtc
      541 ggcgaaggag acgatccgcc gatccagcaa cttttcggct tcgggaaggt tgggggtgag
      601 caccgcggcg cgccggagca gctgccgata tgcgggcaac gcggacgagg atagtagcgg
      661 cgccccggag gaggagcgca gcacggggtc gaggacgagg ggaagatcgc ggtgcgcggc
      721 gacgaaggag agaatcgcct ccgcggcggc gcgggtcccc accatcccga ccttgacggc
      781 cccgaggggg aaactcttcg cggcggcggc aagcgattca tccaggacgt cgcgcgggac
      841 ggacgcgaag cgggagaagg aggcggtggt ctgcaccgtg aggcaggaga cgaccgcgct
      901 ggcgtgaacc ccggccgccg ccgccgcgcg cgaatccatc aacacacccg cgcccccggt
      961 cggatcgaac cccccgaacg cgaggatcac cggcatcccg ttccgttttc ccatggcggt
     1021 cacggtccct ttctcttccg ggccttcggc gccagcccga gctccgcgat cttcttccgc
     1081 agcgtgttcc ggtggatccc caggatggcc gctgcgcgga tctggttccc cgccgtcgcc
     1141 tcgagaacga ccttcaggag agggcactcc acctgtcgga cgaacagctc gtgaaggcgg
     1201 gtttcggagt caagcgccgg tccgagccgc cggaggaact cccggatccg ttcctcgacc
     1261 tgttgctcca tcggcgagga cgaggtctcg ggcgcgcctt tctccctggt cagttcccgc
     1321 accacgtcgt cgcgccgcag cagttttccc ggggagagga ccgccaggcg ctggacgaag
     1381 ttctcgagtt cccgcacgtt tcccttccat gggcgggcgg agagctcctc gaccgcctcc
     1441 ttcgagaaag agcgcggcgg gcgggagagg acggcgcaat atttttgcag gaaatagtcc
     1501 gccagcagcg ggatgtcctc cttcctctcg gcgagggaag ggacccgcag ggggaagacg
     1561 ttcagccggt cgaagaggtc ctcccggaac tttccgtccg acaccatctt ctgcaggttg
     1621 cgattcgtcg ccgcgatgat tcgtccgcgg aatttccgcg gctggtcgga tcccaccgga
     1681 gcgtactccc gctcctgcag gaaacggagc agcttggcct gcaacccgcc gggcgtatcg
     1741 ccgatttcgt cgaggaagag ggtgccgtcc atggcggcgg aaagtttccc cgggcgagcg
     1801 ctttccgctc cggtgaacgc ccccttctcg tgtccgaaca gctccgcctc ctggaggtcg
     1861 cgggggatcg cggggatgtt caccgcaacg aagggacccg ttcgtctccc caggtcgtgg
     1921 atgcaccggg cgatcaactc cttccccacg ccgcgttctc ccaggagaag aatggtggcg
     1981 tccgagtcgg ccaccttccc gattttctgg aacacctcga ggatggcgcg gcttttccct
     2041 acgatccggg ctgaggacca ttcttcccgc tcctcggggc ggaaggaact ctccctggag
     2101 gacgactcct gcgccacgtt gcgaaggagt tcctcgatgc gggagagttc gaacggcttg
     2161 gtgatgaact cggcggcccc cgcacgggtg gagcgcgcgg cgacatcggg aaggtggacc
     2221 gcggtcatga tgaagaaaag ggtgggagac ttccgcgcct ggacgcgttc gagcacctcg
     2281 atcccctcca ccccgggcat ccggatgtcg acgaaggcgg cggaatacgt attcttcccg
     2341 aggagagtga gagcctgctc tccgtcctcg gcgaggtcgg cggcgaaccc catcccggtg
     2401 acggtcttcc gaagaaccca gcggatgctc tcgtcgtcgt ctgcgatgag gattcgtttc
     2461 acgtcgcggc ctttctcctc ggatcgaccg gaagggatag taataccgcg gttccggcgc
     2521 cgggagaaga ccggatctcc atcttccctc cgagccgcgt caccgcctgc cgggccatca
     2581 ccaggccgag ccccgtgccg cgcgccttcg tggtgtagaa ggggaggagc gccttgtgca
     2641 gctcctcttc ggtcatcccc ttcccgttgt cggtgatctc gacctcgagg aacgaacgct
     2701 tcctgccccg gccccgggcg aaccggtagt tgacgttcat ccgcgcctcg atcccaacgg
     2761 ttcccgcccg ctcgatcgcc tccacggcgt tcttcaatat gttgaggagg gcccggtaga
     2821 cggtgtccgg gtgtccggag atcgggggga ggctcgggtc gacgcggagc tcgaaccgga
     2881 cctcctttcc ctgggcacgg gcctcggaga ggagaagctg ttccgcctcg cgcagcaggg
     2941 ggggtagggc gaagggacgt gggggcgggg gagatttccc catctcgagc atcttctcca
     3001 cgaggtcgtt gatccgccgc gcctcgcgca ggatcagccg gaccccctcg gcgcggtcct
     3061 cgtccgggcc ttccccacgc aggatccact gtgccgcccc gaggatgccg cccaggggat
     3121 tcttgatctc gtgggcgatt ccgtacgcca gcatctgcat ctcctcggcg cggaccgtcg
     3181 cccgctcctc ctggccgacg agcgaaagga tctccgaaga tttgaccgag aggaccgccc
     3241 cctgcgggtc gccggacgcc ccggagagcg gggaggctcc gagcatcacc gggatggaag
     3301 cggccccgcg gcgctgaacc gtctgccccc cgcccaccgg cttcaacgcc acgtcgaagc
     3361 ccgtgaccgg cgtgttctcc tcgatcgcct tccggacgat ccggatcgcc tccgggcttc
     3421 cccggaaaag agtccggaaa tgtttcccgg cgagtgcctg ggaggagcct tgcaggatct
     3481 cttcggcggc ggggttgatg tacgccagct ttcccgcgac gtcgaagacg aggatcccga
     3541 tgttgaccga ctccagggtc tgccggagga ggtcgctcaa aagaagcgct cgacgacggc
     3601 ccggaaagag tccgggtcgg cgatcttcgg cagctccgag cgaaaggcgg cggcgccctg
     3661 gatcccacgg ctgtaccagg cgaggtgttt gcgcatctcc ttgatcccgt gcctcccatg
     3721 gcgcaggaac atctcctcgc cgtggcgcag gatcagggcg cgtcgtgcct cgggggtggg
     3781 gaacggggcg aaggggagcg ttccggcggg gccggcgggg agagcgatct cccgtttcaa
     3841 ggccgcgaag atccaggggt ttcccatggc ggcccgcccg atcatcacgc tgtcgcaccc
//
LOCUS       DASVJL010000316         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1179393, whole genome shotgun sequence.
ACCESSION   DASVJL010000316 DASVJL010000000
VERSION     DASVJL010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179393"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VF357_06695"
     CDS             complement(<1..248)
                     /locus_tag="VF357_06695"
                     /inference="COORDINATES: protein motif:HMM:NF026151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523871.1"
                     /translation="MTEGKRVRRGPFREGEDILLISPKGEEHLIRLTPGKAFGTHKGN
                     LPHEDLIGKEDGGRAWTAMGSEYRAFRPTYMQFIMNQKR"
     gene            complement(245..1264)
                     /locus_tag="VF357_06700"
     CDS             complement(245..1264)
                     /locus_tag="VF357_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523872.1"
                     /translation="MKCKRCRRAEAAVDLPSHHAAFCPDCFFVFFRRQVTEGIRKLRL
                     LAPEDRVLVCVSGGKDSLVLWDILIDEGYETEGLYIDLGIDGYSDRSKGKVLAYAASR
                     GKTPIVVELAKEGIPIPEAARCVRMQECSICGTVKRYFFNRVAAEGKFNVVATGHNLD
                     DETARLLGNLLHWQRDHLARQHPLLPGAGGGLVRKVKPLWRVGEVETAAYGFLKGIDY
                     VTEECPMSEDATSLVYKEALSRIEDRMPGTRIVFYQGFLDPSNPLRKGSETAAPTDAV
                     PMPEDAPVMDALRVTDVDAEEDGGTPRTCASCGAPTYAETCSYCRLKERVRKARSLRA
                     AKVSR"
     gene            complement(1273..1485)
                     /locus_tag="VF357_06705"
     CDS             complement(1273..1485)
                     /locus_tag="VF357_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX7523873.1"
                     /translation="MYNGSMITVRLPQKKKELEVPGPRRVMDLLADAGVRPTTVIVTQ
                     GKKLLTKDHRVEDGATIDVISVVSGG"
BASE COUNT          264 a          630 c          543 g          338 t
ORIGIN      
        1 cgcttctggt tcatgatgaa ctgcatgtag gtggggcgga aggcgcggta ctcgctcccc
       61 atcgcggtcc acgcgcggcc gccgtcctcc ttgccgatca ggtcctcgtg cggcagattc
      121 cccttgtgcg tgccgaacgc cttccccggc gtgagcctga tgaggtgctc ctcccccttg
      181 ggggagatca gcaggatgtc ctctccttcc cggaacggtc ccctgcgaac gcgcttgccc
      241 tccgtcatcg ggaaaccttc gccgcccgca gcgaacgcgc tttccggacg cgctccttca
      301 atcggcagta ggagcacgtc tcggcatacg tcggggcgcc gcacgatgcg cacgtccgcg
      361 gtgttccccc gtcctcttcc gcgtcgacat ccgtcacccg cagggcatcc ataaccggcg
      421 cgtcttccgg catcgggacg gcatcggtcg gcgcagcggt ctccgaccct ttccgcagcg
      481 ggttcgacgg gtcgaggaac ccctggtaga agacgatccg cgtccccggc atccggtcct
      541 cgatgcgcga cagcgcctcc ttgtacacga gggaagtcgc gtcctcgctc atcgggcact
      601 cctcggtcac gtagtcgatc cccttcagga acccgtacgc cgcggtttcg acctcgccca
      661 cgcgccacag cggcttcacc ttgcgcacga gccctccgcc cgccccgggc agcaatggat
      721 gctgccgcgc caggtggtcg cgctgccaat ggagcaggtt ccccagcagc cgggccgtct
      781 cgtcatcgag attgtgcccc gtcgccacca cgttgaactt tccttcggcg gcaacgcggt
      841 tgaagaagta ccgcttcacc gtgccgcaga tggagcactc ctgcattcgc acgcagcggg
      901 ccgcctcggg gatcgggatt ccctccttcg cgagctcgac gacgatcggc gtcttcccgc
      961 gcgacgcagc gtaggcgagg actttccctt tcgaccggtc ggagtacccg tcgatcccga
     1021 ggtcgatgta gagcccctcc gtctcgtatc cctcgtcgat caggatgtcc cagagaacca
     1081 ggctgtcctt gccgcccgac acgcacacga ggacacggtc ctccggggcg aggagccgta
     1141 gcttgcggat cccctccgtc acctgtctcc ggaagaagac gaagaagcag tcggggcaga
     1201 aggcggcgtg gtggctcggc aggtcgaccg cggcctcggc ccgccggcag cgcttgcatt
     1261 tcatcaggtt gttcagcccc cggagacgac ggagatgacg tcgatcgtcg ccccgtcttc
     1321 tacccggtgg tccttcgtga gaagcttttt cccctgggtg acgattaccg ttgtagggcg
     1381 tacccccgca tcggcgagca ggtccatcac ccgccgggga ccggggactt cgagctcttt
     1441 tttcttctgg gggaggcgaa cggtgatcat tgatccatta tacatggagt ctccacggaa
     1501 cagttgagtc tcccccgggc gcggggtaag ataaccgcaa atcatccctc aaggaggaat
     1561 ttcaaaatgc gcgtccgctc cgtggtgctg attgtcgtgt ccgtaatgct tcttgccttc
     1621 gccatcggct gtgccaagca ggaaaacccc ccgcccgcgg ctaccggggc tcccggcgct
     1681 cccggtgctc ccggcgctac cggggctacc gggtctcccg gggataacgc ggctcccggt
     1741 gctcccggtg ctcctggcgc tcccggtgct cccgg
//
LOCUS       DASVJL010000317         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1365449, whole genome shotgun sequence.
ACCESSION   DASVJL010000317 DASVJL010000000
VERSION     DASVJL010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365449"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1638)
                     /locus_tag="VF357_06710"
     CDS             complement(250..1638)
                     /locus_tag="VF357_06710"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7523874.1"
                     /translation="MKEYVLIVDDEQSLRQVLDLFFRKEGFEVDMASSLADAEAAIAA
                     NAYDLVLTDLRMARPDDGLKVLRAAVRKNPWTQVIILPAYATVETAKEAIRYGAYDYL
                     TKPFDTANLRKLVRAALARKEDDAGRRKALRESVQGTSAYEGIVGRSEPILGVFELID
                     RVAPTDANVMILGESGTGKELVAAALHARSPRKVFPFVPINCAAIPETLIESELFGHV
                     RGAFTGAVQLKKGLFETAHRGTLFLDEVGELPQPMQSKLLRSLQERSFRRVGGNEDIF
                     IDIRIVCASKRDLHREMVEGRFRDDLYFRLNVIQVFVPPLRDRREDIPALAVHFLRKF
                     SEKHGKPMARIDGEAMRALLAYDFPGNVRELENLLERATIMETKNVISIGSLPSNVTK
                     FVTQEAAGAGKNQESFPEEGVFLDTEMDRLEKIHLVNALEKTGGNRTEASKLLNISFR
                     SLRYRLQKHGIE"
     gene            complement(1721..>2752)
                     /gene="dprA"
                     /locus_tag="VF357_06715"
     CDS             complement(1721..>2752)
                     /gene="dprA"
                     /locus_tag="VF357_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX7523875.1"
                     /translation="FLRLSLIEGFTVDHLRRLRASGAGGFLPGMTPRGVSLLGKAKAA
                     LGSAEAGRRAEAVREACERRGIAILPWGASDYPVAVREIPGAPLLLYRAGKAWNGGGG
                     VAVVGSRAPTGPGREFARLLSGELAAARWTVVSGMARGIDAAAHKGVLHAGGTTVAVL
                     GCGVDVAYPREHAELRDEILEKGAIFSEYPPGSLPLPHRFPERNRIVSGLSRGVIVAE
                     APERSGALITARLALEQGRDVMVVPGNPWFAHTAGSNRLLREGATPVCSVADVHGVLG
                     CPPPVAAGGVAERVLAALSRELHAGEIAEALSVPVQELLPCLMEMELANLVEKRPGNY
                     YKKLSATVS"
BASE COUNT          441 a          964 c          863 g          484 t
ORIGIN      
        1 atcgcgccga ggttggactt ggcctcggac tgcttcgact tggcctggaa cttcaggaag
       61 ttcgggatgg cgatggcggc caggatgccg atgatcgcga cgacgatcat cagttcgatc
      121 agggtgaacc cctttttgct acgcagcgcg ctcaacatgc ttgtttcctc ctttcgggtc
      181 tggaaagcgt atcgttgatt attcttatcg caaggggcgg gccaaaactt caaggcaatc
      241 ttgtaggggc tattcgatcc cgtgcttctg caaccggtaa cggagcgaac gaaaggaaat
      301 attcaacaat ttcgaggctt ccgtgcgatt tcctcctgtc ttttcgaggg cgtttaccag
      361 gtggatcttt tccaggcggt ccatctccgt gtcaagaaag accccctctt ccgggaatga
      421 ttcctgattc ttccctgccc ccgccgcctc ttgggtgaca aattttgtca cattcgaagg
      481 gagggagccg atcgagatga cattttttgt ctccatgatc gtcgcccgct cgaggaggtt
      541 ttccagctcc cgcacgttgc cggggaagtc gtaggcgagc agcgcccgca tcgcctcgcc
      601 gtcgatccgc gccatcggtt tgccgtgctt ctccgagaat ttccggagga agtggacggc
      661 caacgccggg atgtcctcgc ggcgatcccg caagggggga acgaacacct ggatgacatt
      721 gaggcggaag tagaggtcgt cccggaaccg cccctcgacc atctcccggt gcaggtcccg
      781 cttcgaggcg cagacgatcc ggatgtcgat gaagatgtcc tcgttcccgc cgacccggcg
      841 gaacgaccgc tcctggaggg agcgcagcag cttgctctgc atcggctggg gaagctcgcc
      901 cacctcgtcg aggaagaggg tcccgcggtg cgccgtctcg aagagcccct ttttcaactg
      961 aacggctccg gtgaaggcgc cgcgaacgtg cccgaacagc tcgctctcga tcagcgtctc
     1021 ggggatggcg gcgcagttga tcgggacgaa ggggaacacc ttccgggggc tgcgggcatg
     1081 gagggcggcg gccaccagct ccttccccgt tccgctctcg cccaggatca tcacgttggc
     1141 gtcggtgggg gcgacccggt cgatcagctc gaagaccccc aggatcggct cgctgcgtcc
     1201 gacgatccct tcgtacgcgg aggtcccctg gaccgattcg cgcaacgcct tccggcggcc
     1261 ggcgtcgtct tccttccggg cgagggcggc gcgcaccagc ttgcggaggt tcgcggtgtc
     1321 gaacggcttg gtgaggtagt cgtacgcccc gtaccggatc gcctccttgg ccgtctcgac
     1381 ggtcgcgtag gcggggagta tgatcacctg ggtccacgga tttttccgca cggccgcgcg
     1441 caataccttc aacccgtcgt cgggccgcgc catccgcagg tcggtgagga cgaggtcgta
     1501 cgcgttcgcc gcgatcgcgg cttccgcgtc ggcaagggag gaggccatgt cgacctcgaa
     1561 cccctccttc ctgaagaaga gatccagtac ctgccgaagg ctctgctcgt cgtcgacgat
     1621 gaggacgtac tccttcaacc cgcctccttt tccctggggg ggcggttctc ccgcccgccc
     1681 cttgcacccc ttccgcaaac acgcggcccg aaccgcgggg tcaactcacg gttgcggaca
     1741 acttcttata gtaattcccc ggcctttttt ccaccagatt cgccagttcc atttccatca
     1801 ggcacggaag gagctcctgg accggcaccg acagggcctc cgcaatctcc cccgcgtgaa
     1861 gttcccggga gagagcggcg agcacccgct ccgcgacccc gccggcggca acggggggcg
     1921 gacacccgag aactccatga acgtccgcga ccgagcagac cggggtcgcc ccctcccgca
     1981 gcaggcggtt gctcccggcc gtgtgggcga accaggggtt cccgggtacg accatcacgt
     2041 cgcgcccctg ctcgagcgca agcctcgcgg tgatcagcgc gccgctgcgc tccggcgcct
     2101 cggccacgat caccccgcgc gacaacccgc tcacgatccg gttccgctcc gggaaccggt
     2161 gcggcagggg caacgatccc ggcgggtatt ccgagaagat cgcccccttt tcgaggatct
     2221 cgtcccgcaa ttccgcgtgc tcccgggggt acgccacgtc cacgccgcac ccgaggaccg
     2281 ccaccgtcgt ccctccggcg tgaaggaccc ccttgtgtgc cgccgcgtcg atcccgcgcg
     2341 ccatcccgct gaccaccgtc catcgggccg cggcgagctc ccccgacagg aggcgggcga
     2401 attccctccc aggtcccgtg ggggcgcggc tcccgaccac ggccaccccc ccgccgccgt
     2461 tccatgcctt tccggcccgg tacaaaagca gcggcgcccc ggggatctcc cgaaccgcga
     2521 cggggtagtc gctcgcgccc caggggagga tcgcgatccc ccgccgctcg cacgcttccc
     2581 gcacggcttc cgcccgccgg cccgcctccg cggagccgag cgccgccttc gccttcccga
     2641 gaagggaaac gccccgcggc gtcatgccgg ggaggaaacc gccggccccg gaggcgcgga
     2701 ggcgccggag atgatcgacg gtgaacccct cgatcagcga cagtcggagg aa
//
LOCUS       DASVJL010000318         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1554195, whole genome shotgun sequence.
ACCESSION   DASVJL010000318 DASVJL010000000
VERSION     DASVJL010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554195"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VF357_06720"
     CDS             complement(<1..495)
                     /locus_tag="VF357_06720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7523876.1"
                     /translation="MNSSAVLLFLLSFGHMCTDIVQGALPALLPFLKERFDLSYAVTG
                     TLLMAAHLTSSVIQPLFGYVTDRRPFPILLPLGCAISGVGIALVPLSPSFGWLVAFIM
                     FTGLGTAAFHPEGFKATACIASERRATGMSFFSVGGNLGFAIGSPAAIFLVSRYGLPG
                     ASGLL"
     gene            complement(492..1508)
                     /gene="epmA"
                     /locus_tag="VF357_06725"
     CDS             complement(492..1508)
                     /gene="epmA"
                     /locus_tag="VF357_06725"
                     /inference="COORDINATES: protein motif:HMM:NF006828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEX7523877.1"
                     /translation="MPRERTDRETWRRLADGELSWEALRARARVLEGIRAFFRDRGFL
                     EVDPPIAQAYPNIDPNIFPVKITDAFGKEARFYLHTSPELAMKKMLAAGSGDIFFIGK
                     VFRDREGSPLHSPEFTMLEWYRVGGDADAVMRDVEELVRTLARTISGEAVVHGNGREI
                     PVDGPWSRWELDDAFRELLGVGMAGEKELREALDRKGLRPGADESWEDLFHRACLDVV
                     EPALAERGACFLTGYPAPLAAMARRRPGRPEVSERFEGFVAGVELVNGYEELTDPAEQ
                     EARLLALADRHRRSTGETLPVDPGFLAAMRHGLPPCAGAALGVDRLVMLLLGKDDIAD
                     GMYR"
     gene            complement(1572..2669)
                     /locus_tag="VF357_06730"
     CDS             complement(1572..2669)
                     /locus_tag="VF357_06730"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX7523878.1"
                     /translation="MSPARAVLLVFALLAGAFLLPSPSPAAGPVSLPALAPLPLPTSP
                     PENPTTPEKVNLGKMLFFDRRLSGDGTMNCATCHDPENGFADALPISLSYPTTRNWRN
                     SPGLVNVAYRKTLFHDGRATSLEEQALFPMMSPFEMNRNLDYLEEVLKTVPAYVEAFR
                     SVFGGEITRQRVAMAIAAFERTLLSRDTPLDRHLRGEPGALSARQRAGLELFLGKAGC
                     ATCHNGPNLTDERFHNLGVPEDPKAKEDPRVLATVRFVGKVSGFPDYRTLREDPGRFL
                     VTKDPADWKAFATPALREVAATAPYMHNGALGTLEEVIDFYDRGGGDDPKKSPMLRPL
                     GLSREEKESLREFLATGLSGKIPEARAPVLP"
BASE COUNT          455 a          946 c          970 g          412 t
ORIGIN      
        1 gaggaggccg gacgcccccg ggagcccgta cctggagacg aggaagatcg ccgccgggga
       61 gccgatggcg aacccgaggt tccccccgac ggagaagaag gacatccccg tggcccggcg
      121 ctcggaagcg atgcacgcgg tcgccttgaa cccctccggg tggaacgccg cggtgcccaa
      181 ccccgtgaac atgatgaacg ccaccagcca gccgaacgac ggggagagcg gaaccagcgc
      241 gatcccgacg ccggagatcg cgcaccccag cggcagcagg atcgggaagg ggcgccggtc
      301 cgtgacgtag ccgaacagcg gctggatcac ggacgaggtg aggtgcgccg ccatgagcag
      361 cgtccccgtg acggcgtacg aaaggtcgaa ccgctccttg aggaacggga gaagcgccgg
      421 cagggccccc tgcacgatgt ccgtgcacat gtgcccgaag gagaggagga agagcaggac
      481 ggcggaggag ttcaccggta catcccgtcc gcgatgtcgt ccttgcccag caggagcatg
      541 acgagccggt cgacccccag cgcggccccc gcgcaggggg ggaggccgtg gcgcatcgcg
      601 gcgaggaacc ccgggtcgac ggggagcgtc tcgccggtcg atcgccggtg gcggtcggcc
      661 agcgcaagca ggcgggcctc ctgctccgcg gggtcggtca gctcctcgta cccgttgacg
      721 agttcgacgc ccgccacgaa cccctcgaat cgctccgaca cctcggggcg gccggggcgg
      781 cggcgggcca tcgcggcgag gggcgcgggg tagcccgtga ggaaacaggc gccacgttcc
      841 gcgagagcgg gctccaccac gtcgaggcag gcccggtgga agaggtcctc ccacgattcg
      901 tccgctcccg gacgcaaccc cttgcggtcc agcgcttcgc gcaactcctt ctcccccgcc
      961 atcccgacac cgaggagttc ccggaaggcg tcgtcgagtt cccatcggga ccatggcccg
     1021 tcgaccggga tctccctccc gttgccgtga acgaccgcct ctccggaaat cgttcgggcc
     1081 agcgtccgga ccagctcctc gacgtcccgc atcacggcat ccgcgtcgcc gcccacccgg
     1141 taccattcga gcatcgtgaa ctcgggggag tggaggggcg acccttcacg atcccggaac
     1201 accttcccga tgaagaagat gtcgcccgat ccggcggcga gcatcttctt catcgccagt
     1261 tcgggagagg tgtgcaggta gaaccgcgcc tccttgccga aggcgtcggt gatcttcacc
     1321 gggaagatgt tcgggtcgat gttcgggtac gcctgcgcga tcggggggtc cacctcgagg
     1381 aacccccggt cccggaagaa ggcccggatt ccctcgagga cccgggcccg ggcccgaagc
     1441 gcctcccacg acagctcccc gtccgcgagc cgccgccacg tctcgcggtc tgttctttcc
     1501 cgaggcatcc gtattgttta cccttgatcc gccccgccgt aaagcgtcat aaccggttac
     1561 ggcaagagat ctcaggggag gacgggcgcg cgcgcttccg gtatcttgcc cgaaagcccc
     1621 gtggcgagga attcccggag ggactccttt tcttccctcg acagcccgag cggccgcagc
     1681 atgggcgatt tcttcggatc gtcgcctccg ccgcggtcgt agaagtcgat cacctcctcg
     1741 agggtcccga gggcgccgtt gtgcatgtag ggggcggttg ccgccacctc ccgcagggcg
     1801 ggcgtggcga acgccttcca gtccgccgga tccttcgtga ccaggaaccg cccggggtcc
     1861 tcgcggagcg tccggtagtc cgggaatccc gacaccttgc cgacgaatcg caccgtcgcg
     1921 agcaccctcg gatcctcctt cgctttcggg tcctccggga cgccgaggtt gtggaaccgc
     1981 tcgtcggtca ggttcgggcc gttatggcac gtcgcgcaac cggccttgcc gaggaacagt
     2041 tcaagcccgg cgcgctgccg cgccgatagc gcccccggct ccccgcgcag gtgacggtcc
     2101 agcggcgtgt cgcgggagag gagggtccgc tcgaaggcgg cgatcgccat cgccacgcgc
     2161 tgccgggtga tctctccccc gaagacggat cgaaacgctt ccacgtaggc ggggaccgtt
     2221 ttcagcacct cctcgagata gtcgagattc cggttcatct cgaaggggct catcatcggg
     2281 aaaagggcct gctcctcgag ggaggtcgcg cgaccgtcgt ggaacagcgt cttccggtac
     2341 gcgacgttca cgagccccgg ggaattgcgc cagttccgcg tggtggggta ggagagggag
     2401 atcggaagcg cgtcggcgaa cccgttctcg gggtcgtggc aggtggcgca gttcatcgtc
     2461 ccgtcccccg agaggcgccg gtcgaagaac agcattttcc cgaggttgac cttctccggg
     2521 gtggtcgggt tctccggggg agacgtcggg agaggaagcg gggcgagggc gggcagcgaa
     2581 acgggccccg ccgcgggcga gggggaagga agaaggaacg ctcccgcgag gagcgcgaaa
     2641 acaaggagaa cggcgcgcgc ggggctcatg gtttcttccc tcccggcccg gcggccttga
     2701 gctccctgtc gatgaccgcc ctgtactcct cgatcggccg gttcccgagg agtttcaccc
     2761 cgttgatgaa gatcgtcggc gtg
//
LOCUS       DASVJL010000319         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_708959, whole genome shotgun sequence.
ACCESSION   DASVJL010000319 DASVJL010000000
VERSION     DASVJL010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708959"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VF357_06735"
     CDS             complement(<1..186)
                     /locus_tag="VF357_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523879.1"
                     /translation="MSDTSPRSHKARRLLSLFLLAVFLPLVSGCLFGEKRRTFSPPDE
                     ELLRAKADPRLRKEAETG"
     gene            complement(158..682)
                     /locus_tag="VF357_06740"
     CDS             complement(158..682)
                     /locus_tag="VF357_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523880.1"
                     /translation="MLREREKRVLVIGGAAAAALLLLVFAVIPGISQIKSQTRAVAAA
                     ENDLAEVRKARPEIQRIQGETGAKAGIVQTAANVKDAPLSRITSALQEAGIPQAALNI
                     KSGGARDGEKFREESFDVRIENLTYLEAVKMLQRLSAGSLPVTIRSASLKSRYDDPRY
                     LDVTLRVGYLTPKS"
     gene            complement(672..2045)
                     /locus_tag="VF357_06745"
     CDS             complement(672..2045)
                     /locus_tag="VF357_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523881.1"
                     /translation="MNTIGISISRDRICAVALEGTTPGFRIAAAVSVPCAEPFGTAGD
                     AAALSAALREALPGVPLPGAVLTLPPPITFLRPMTLPVTDLPRARAIHLGELEGSLPI
                     EDEEILSDLLPAPPEGSGLFFAVAARRSFVESASEHFGTAGIRVDRVVTDPVALLLLA
                     PDVAGSPRDGIYLSTFNDVLLLRVSGGGVTAARQFPQTMTDRPEELLDAIREAATEAG
                     GAPLDVFLIGEMPAPLAAALPYAVRVPLPDGVSSTHLAAYGAALAPLRPDVGGGFSLR
                     TSAEAALEQERDHRWKLATGIAAGVAVLLALGAFAFASWTEGEKAARARALVKKEFAA
                     VAPEVRNVVQAGTQIRAKLDSLRREQKELGTDAPPPVDLLQLASRAIPKGEIAVREIS
                     IEGGRMRIAGDAGSDARLVEAYRAGLASAFGPNYSATVQESGGSVKGTSVTFTILVEK
                     KGERRAS"
     gene            complement(2055..2924)
                     /locus_tag="VF357_06750"
     CDS             complement(2055..2924)
                     /locus_tag="VF357_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523882.1"
                     /translation="MPGNPKPRIPGGLRGRAIVAIGALLFLASLSSAVALTLPSDAIL
                     STLRPMLRDHGVELSAQEARFLFPLGIRLSGVTLAFPGNPPVALDEIVATWEWTGLFR
                     WAPSRLRFRKGAASGDLRFSPAFWSPESGRILLSGVSSSDLPLSVFSTSGAGFSIRMV
                     EARWNVSRGMVMADGSGTLDFLQVPVPAPDSPIREARIEGVSLSFLIRENAFRVPKLA
                     GTFEGSQVDGTGEITRVLSPRQSMMTFHLRIRNPFEGRVARLFDMLSKNAKNANLRII
                     GTLTAPMGEFQFF"
     gene            complement(2914..4539)
                     /locus_tag="VF357_06755"
     CDS             complement(2914..4539)
                     /locus_tag="VF357_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523883.1"
                     /translation="MIGFRSRFLLVIAATVGLAAAAWAEGIFPVNNTGVGNWVFDQPS
                     VVASGSTLYLAFVGNDAAGALNIDGTANTSLNTRLYYAAVNGGADFKNKATTRTQVLL
                     TAPVAIDSGDAYTNARHPQIALRSTTELVILFQAIPAGETNYKLFRALVTISGKAVAS
                     QSVAEIRDAAGARIPGKLTDPSFALVTSDNTLRIAFSSFPSPLVDNSTFTDVYYARVG
                     WDAARVLNNTILLTTTASTTGVSPIPRLRLDSNRFSHIVWAANNSTGDPTGIYYAMVH
                     AVSPSVVDNLAIGATQVLSGGNRWGFPSVVLTSAQAVWILASDVFQSGGSTGLADSVG
                     ITEVNPYAVTRDGNPVSVNNIPGGSNISFFRNPPGGSVLSADFDAYHPEVALDSQNRM
                     HVAGYGFRSNVPPFQGTPGRYYVMSLGSTVSGAGTSSIFASMVASPVAVGTGDLAFAM
                     ELPGDYTRPAFVHFSGKSVNLWSGPDNVVTGARNLYVTSTFSPSDPTSQSGCSMVDDP
                     RRDEAGRIPATAVLLLPAAFLALRRATRKAFAR"
     gene            complement(4571..>4919)
                     /locus_tag="VF357_06760"
     CDS             complement(4571..>4919)
                     /locus_tag="VF357_06760"
                     /inference="COORDINATES: protein motif:HMM:NF015867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HEX7523884.1"
                     /translation="VSSSGMVNINTAPKEVLMSLSAGTDLAVGAIDAKTADEIIEYRK
                     DHPFTDSSQIGNVSPFLRDLFARTLIRNMVGIQSTYFHVRSSGNVGGTVRTIDAIGIR
                     VQNEVQWRFWRIE"
BASE COUNT          842 a         1580 c         1723 g          774 t
ORIGIN      
        1 gcccgtctcc gcctccttgc gcaacctcgg gtcggccttg gcgcggagaa gttcttcgtc
       61 cgggggagag aaggtccgtc gcttctcccc gaacaggcac ccggagacca acggcaggaa
      121 caccgcgagc aggaagaggg aaaggaggcg cctggcctta tgacttcggg gtgaggtatc
      181 cgacacgaag ggtcacgtcg aggtagcggg ggtcgtcgta gcggctcttg agggacgcgg
      241 agcggatggt caccggcagc gatcccgcag acagccgctg cagcatcttg acggcctcga
      301 ggtatgtgag attctcgatc cggacatcga acgactcctc ccggaacttt tcgccgtcgc
      361 gcgcgccgcc cgacttgatg ttcagcgccg cctgagggat ccccgcttcc tggagcgcgg
      421 aggtgatcct cgacaagggg gcgtccttca cgttggcggc ggtctggacg atgcccgctt
      481 tcgcgccggt ttcaccctgg atccgctgga tctcggggcg cgccttgcgc acctcggcga
      541 ggtcgttctc ggccgcggcg accgcgcgcg tctgcgactt gatctgcgag atccccggga
      601 tcacggcaaa gacaaggagg agaagggcgg cggccgcggc ccctccgatc acaaggaccc
      661 gcttctcacg ttcacgaagc acggcgttcc ccctttttct cgacgaggat ggtgaaggtg
      721 acggacgtgc ccttcacgct tcccccggac tcctgcacgg tcgccgaata gtttggaccg
      781 aacgcgctcg ccaggccggc gcggtacgcc tcgaccaggc gcgcgtcgct ccccgcgtcc
      841 cccgcgatcc gcatgcggcc cccctcgatc gagatctccc gtaccgcgat ctcccccttg
      901 ggaatcgccc gggaggcgag ctggagaaga tcgacgggcg ggggagcgtc ggtcccgagc
      961 tccttctgct cgcgccggag ggagtcgagc ttcgcgcgga tctgggttcc cgcctgaacg
     1021 acgttgcgaa cttccggggc gaccgccgcg aactccttct tcacgagggc gcgcgcgcgg
     1081 gccgcctttt ccccctcggt ccagctggcg aaagcgaacg cccccagggc gaggaggacg
     1141 gcgacgcccg cggcgatccc ggtggccagc ttccatcgat gatcccgctc ctgttccagc
     1201 gcggcctccg cagacgtccg cagcgaaaag cctcccccga cgtccgggcg cagcggggcc
     1261 agcgccgccc cgtacgcggc aagatgggtg gaggaaactc cgtcgggaag cgggacccgt
     1321 acggcgtagg gaagggcggc cgcaagcggc gcgggcattt caccgatcag gaatacatcg
     1381 agcggcgcgc cgccggcctc cgtcgcggcc tcccgaatcg cgtccaggag ctcctcgggg
     1441 cggtcggtca tcgtctgggg gaactgccgc gccgccgtca ccccgcctcc ggagacgcga
     1501 agcagcagga cgtcgttgaa ggtcgaaagg tagatcccgt cccgcggcga cccggcaaca
     1561 tcgggggcga gaagaagaag ggcgaccgga tcggtgacga cgcggtccac ccggatcccg
     1621 gcggtcccga aatgctccga ggcgctttcc acgaacgatc gccgcgccgc gaccgcgaag
     1681 aagagccccg atccttccgg cggggccggc aggaggtcgg acaggatctc ctcgtcctcg
     1741 atcggcaggc tcccctcgag ttcgcccagg tggatcgccc tcgccctcgg aagatcggtc
     1801 accgggagcg tcatcggtcg gaggaaagtg atcggcggcg gcagcgtcag caccgcgccc
     1861 ggaaggggga cccccggcag cgcctcgcgg agcgccgccg aaagcgcggc cgcgtccccg
     1921 gcggttccga acggttccgc gcacggaacg gacacggccg ccgcgatccg gaagccgggt
     1981 gtcgttcctt cgagcgcgac ggcgcagatc cggtcgcggg agatggagat tccgatcgtg
     2041 ttcatggtca cctgtcagaa gaactggaat tcgcccatcg gggcggtcag cgtgccgatg
     2101 atcctaagat tagcattctt tgcgtttttc gacagcatgt cgaacaaccg ggcgacgcgg
     2161 ccctcgaacg ggttccggat cctgaggtga aacgtcatca tcgactgccg aggcgacagg
     2221 acccgcgtga tctctcccgt gccgtccacc tgcgacccct cgaacgtccc cgcgagtttc
     2281 gggacccgga aggcgttctc ccggatcagg aacgagaggg agaccccctc gatccgcgct
     2341 tcccggatcg gggaatcggg cgcggggacg ggaacctgga ggaagtcgag cgtccccgac
     2401 ccgtccgcca tcaccattcc acggctcacg ttccagcggg cctcgaccat gcggatcgaa
     2461 aaccccgccc cggacgtcga gaagaccgaa agcgggagat cggacgagga aacgcccgac
     2521 agtagaatgc gcccgctctc cgggctccag aaggcagggg agaagcgcag gtccccggaa
     2581 gcggcgccct tacggaaccg cagccgcgag ggagcccatc ggaacaaccc ggtccactcc
     2641 caggttgcga cgatctcgtc cagagcgacg ggcgggttcc cggggaaggc gagggtgacc
     2701 cccgagagac ggatccccaa agggaagagg aaccgggcct cctgcgccga cagttcgact
     2761 ccgtgatccc ggagcatggg ccggagggtg gacaggatcg cgtccgacgg cagcgtgagg
     2821 gccaccgcgg aggagagcga ggccaggaac aggagcgcgc cgatcgcgac gatcgcgcgg
     2881 ccccggagcc cccccgggat ccggggcttg gggttaccgg gcaaatgcct tccgggtcgc
     2941 tctccggagc gcaaggaagg ccgcggggag caggagcacg gcggtggccg ggatccggcc
     3001 cgcttcgtcg cgacgcgggt cgtcgaccat cgagcatccc gactgactcg tgggatcgct
     3061 gggcgagaag gtgctggtga cgtagaggtt acgcgcaccc gtgacgacgt tgtccggccc
     3121 cgaccagagg ttgaccgatt tcccgctgaa atggacgaat gccggccgcg tgtagtcccc
     3181 ggggagctcc atggcgaacg cgaggtcgcc ggtgcccacg gcgacggggg aagcgaccat
     3241 gctggcgaag atgctggagg tcccggcgcc cgacaccgtg ctcccgaggg acatcacgta
     3301 gtaccttccg ggagttccct ggaaaggcgg gacgttgctg cggaatccgt atccggcgac
     3361 gtgcatccgg ttctggctgt cgagcgccac ctcggggtga tacgcgtcga agtcggcgga
     3421 caacacggat cctcccggcg ggttgcggaa gaaggagatg ttcgatccgc cggggatgtt
     3481 gttgacgctc accgggtttc cgtcacgcgt gacggcgtac gggttgacct cggtgattcc
     3541 gacggaatcc gccaaccctg tcgatccccc ggactggaat acgtcggagg cgaggatcca
     3601 gacggcctgg gcgcttgtca ggaccacgct ggggaatccc cagcggtttc cgcccgagag
     3661 gacctgggtg gcgccgatcg caaggttgtc cacgacgctg ggtgagaccg cgtgcaccat
     3721 cgcgtaatag attcccgtgg ggtcgcccgt ggaattgttg gccgcccaga cgatgtggct
     3781 gaacctgttg ctgtcgagcc ggagacgcgg gatgggggat acgccggtgg tgctggccgt
     3841 cgtcgtcagg aggatcgtgt tgttgagcac ccgggcggca tcccatccca cacgtgcgta
     3901 gtacacgtcc gtgaacgttg aattgtcgac caggggggaa gggaaagagg aaaaggcgat
     3961 ccgcagggtg ttgtcggaag tgacaagggc aaaggaggga tcggtcagtt tcccggggat
     4021 tcttgcgccc gcggcgtccc ggatctcggc cacggactgg gatgccacgg ccttgccgga
     4081 gatcgtcacc agggcgcgga agagcttgta gttcgtctcc ccggcgggga tcgcctggaa
     4141 gaggatcacc agctcggtcg tggatcgaag cgcgatctgc gggtgacggg cgttggtgta
     4201 ggcgtcgccg ctgtcgatcg ccacgggagc ggtgaggaga acctgggttc gcgtcgtcgc
     4261 cttgttcttg aagtccgctc caccgttcac cgccgcgtaa taaagccggg tgttgagcga
     4321 agtgttggcg gtgccgtcga tgttgagggc gccggccgcg tcgttgccca cgaaggccag
     4381 gtaaagggtc gatccgctgg ccacgacgga gggctggtcg aacacccagt ttccgactcc
     4441 ggtgttgttc accgggaaga tcccttcggc ccacgcggcc gcggcaaggc cgacggtggc
     4501 tgcgatcacg agcaggaaac ggctgcgaaa accgatcatc ggatccctcc gggggtggtt
     4561 gttggcggct ctactcgatg cgccagaagc gccattgtac ctcattttgc acgcgaatac
     4621 cgatggcgtc gatcgtccgt acggtccccc cgacgtttcc ggacgagcgc acgtggaaat
     4681 acgtcgattg aatccccacc atgttccgga tcaaggttcg ggcgaacaga tcccgcagga
     4741 acggggagac gtttccgatc tggctcgaat ccgtgaatgg gtggtccttc cggtattcga
     4801 tgatctcgtc cgcggtcttc gcgtcgatcg ccccaaccgc caggtctgtt ccggccgaga
     4861 gggacatcag cacctccttc ggcgcggtgt tgatgttcac catgccggag gaggagacg
//
LOCUS       DASVJL010000320         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_522297, whole genome shotgun sequence.
ACCESSION   DASVJL010000320 DASVJL010000000
VERSION     DASVJL010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522297"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1607)
                     /locus_tag="VF357_06765"
     CDS             complement(<1..>1607)
                     /locus_tag="VF357_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7523885.1"
                     /translation="FLEKPTWGEVFSDTINTGIYIVEPEVLDLIPPKKNWDFSKNLFP
                     AMLARSDRLFGYVAEGYWKDVGNLDEYLNVHLDLLAGKVKIGFEGEKAGERSVWIGEG
                     TKVDYTAELSNVLLGKKCVVGSGVTMSNVVVGDGCVIEDGAAIQSSVLWERVSVGKGA
                     RIFENIVGSDVRVGRGAFLAERAVISDHCVIGTEAVVKPNVKVWPHKVVEDGAVLSSS
                     LIWGEKWARSLFGAYGIVGLANIEISPEFAAKVGAAYAATFGRKVVLSTSRDSHKASR
                     MINRSIMTGMLSIGVDVHDYGVTPLPVVRYLSRSHGEERGGVHVRKSPFNASFLDLKF
                     FDDSGLDLPMGLEKNIENMFFREDFVRADIEETGGITFPVGGFDLYIDGFVKSVDARV
                     IKERNYNIVLDYSYGSSAVIFPRILGKLGVETVALNAILDPARITRSQEEFDKGMGHL
                     AAISRSLSADFGAMLDTGGEKIFLIDEKGDLLSDATALQVVCLLACRQAGKGLVGVPV
                     TASRNVEKIAARFGMDVVRTRTLPRSL"
BASE COUNT          279 a          571 c          477 g          280 t
ORIGIN      
        1 atcagcgacc gcggcagcgt ccgcgtccgg accacgtcca tcccgaagcg cgccgcgatc
       61 ttctccacat tccgcgatgc ggtgaccggg actcccacca gcccctttcc cgcctggcgg
      121 caggcgagga ggcagaccac ctgcagcgcc gtcgcgtcgg agaggaggtc ccccttctcg
      181 tcgatcagga agatcttctc gccgccggtg tcgagcatcg ccccgaagtc ggcggacagg
      241 gagcgcgaga tcgccgcgag gtggcccatc cccttgtcga actcctcctg cgaccgggtg
      301 atgcgggccg ggtcgaggat ggcgttcagc gccaccgtct ccaccccgag ctttccgagg
      361 atccgtggga agatgacggc ggaggaaccg taggagtagt cgagcacgat gttgtagttc
      421 cgctccttga tcacccgcgc gtcgacggat ttcacgaacc cgtcgatgta gaggtcgaac
      481 ccgcccaccg ggaaggtgat cccccccgtc tcctcgatgt cggcccggac gaagtcctcc
      541 cggaagaaca tgttttcgat gttcttctcc agccccatcg gcaggtcgag gccggaatcg
      601 tcgaagaatt tcaggtcgag gaaagacgcg ttgaacgggc tcttccggac gtggacgccg
      661 ccccgctcct caccgtgaga gcgcgaaagg taacgcacca cagggagcgg cgtgacgccg
      721 tagtcgtgca cgtcgacacc gatcgagagc atccccgtca tgatcgagcg gttgatcatc
      781 cgcgacgcct tgtggctgtc ccggctggtg gagaggacca ccttccttcc gaacgtggcg
      841 gcgtaggcgg ccccgacctt cgccgcgaac tcgggggaga tctcgatatt cgccagcccg
      901 acgatcccgt aggcgccgaa gagggagcgc gcccacttct ccccccagat gagggaagac
      961 gacaggaccg ccccgtcctc gaccactttg tggggccaca ccttcacgtt cggcttgacc
     1021 acggcctcgg tcccgatgac gcaatggtcg ctgatcaccg ccctctccgc gaggaaggcg
     1081 ccgcgcccca cccggacgtc ggatccgacg atgttctcga agatccgggc ccctttcccc
     1141 acggacacgc gctcccacag cacggaggat tggatggccg ccccatcctc gatgacgcat
     1201 ccgtctccga ccacgacgtt gctcatcgtc accccggacc cgacgacgca cttcttcccg
     1261 aggagcacgt tcgaaagctc cgccgtgtag tcgactttcg tcccctcgcc gatccaaacg
     1321 ctccgctccc ccgccttctc cccctcgaag ccgatcttca ccttccccgc caggaggtcg
     1381 aggtggacgt tcaggtactc gtcgagattc cccacgtcct tccagtaccc ctccgcaacg
     1441 tagccgaaca ggcggtcgct ccgggccagc atggcgggaa agaggttctt gctgaagtcc
     1501 cagttcttct tcggcgggat gaggtcgagc acttccggct cgacgatgta gatccccgtg
     1561 ttgatcgtgt cggagaagac ctccccccac gtcggcttct cgaggaa
//
LOCUS       DASVJL010000321         3655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_428120, whole genome shotgun sequence.
ACCESSION   DASVJL010000321 DASVJL010000000
VERSION     DASVJL010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428120"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1537
                     /gene="accC"
                     /locus_tag="VF357_06770"
     CDS             26..1537
                     /gene="accC"
                     /locus_tag="VF357_06770"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX7523886.1"
                     /translation="MFRKILIANRGEIAVRVLRACKEMGIRTVAVFSEVDRKALHTRY
                     ADEAYCIGPAPARESYLVIDKIIDVAKRTGAEAIHPGYGFLAENPLFADRCEKEKIKL
                     IGPSAYAMRTMGSKTLARKTVQAAGVPVVPGTVEPITTEAEVFRVAKEIGFPVMLKAT
                     AGGGGKGMRLVREESELSSSLRMAKSEAKSAFSDDSVYIEKYIENPRRVEIQLLGDRH
                     GNYAHLCERECSIQRRHQKVIEESPSVIVTPEMRAAMGKVAIEAARAVKYEGAGTCEF
                     LVDAKRNFYFLEMNTRLQVEHPVTEMVTGIDIVKEQIRVAAGEELSFRQEDVRQTGHA
                     IECRIYAEDPDSNFMPCPGVVTSLRIPGGPGVRDDSGVYAGFEIPIYYDPIISKLVTW
                     GKDRTEAIARMKRALGVYVVTGVKTTLPFHIRVMNNRHFIEGNFDTNFIEQVFFKEEE
                     ARRLENGELALVTAAIQAFGEERRSALAQQPGEAGAPVSMWKYSTRPGIRKIG"
     gene            1546..2049
                     /locus_tag="VF357_06775"
     CDS             1546..2049
                     /locus_tag="VF357_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7523887.1"
                     /translation="MNYVATIGEKDVKVSVEELGGSNYKVTIDGVEHVVDAQLITDNL
                     WSVLYGNGSFEVDVTRLPAEEYEVLIKGDCHKFTLMNEQRRAMIRAGGKGSAGKAMLT
                     SPMPGKVVKLLVKEGQEVEAGEGVIVVEAMKMENELKSALAGKVKEIFVEEGQVVESG
                     AKLLLVE"
     gene            2059..>3655
                     /locus_tag="VF357_06780"
     CDS             2059..>3655
                     /locus_tag="VF357_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX7523888.1"
                     /translation="MAGKNGKGGLREARERWERDILAPAMSKSRERRPRFESTSGEEV
                     PRLYTPEHLASFDYLRDAGYPGEFPFTRGVQPTMYRGRFWTMRQYAGFGDAKESNRRY
                     RYLLDNGQTGLSVAFDLPTQMGYDSDALMSFGEVGKVGVAIDSLEDMEVLFDRIPLGK
                     VSTSMTINSTAAILLAMYIAVGEKQGVAAAALNGTIQNDILKEYIARGTYIFPPAPSM
                     RIITDIFAFCKDAVPRWNTISISGYHIREAGSTAAQEVAFTLANGIAYVQAAIDAGMS
                     VDSFASRLAFFFNAHNNFLEEVAKFRAARRLWAGIMRERFGAKDPRSWMLRFHTQTAG
                     SSLTAQQPDNNIVRVTIQALSAVLGGTQSLHTNSRDEALSLPTEASVRIALRTQQLIA
                     HESGVAETVDPLGGSWCVEALTSGIEQQAREYIARIDRMGGVIPALESGYIQKEIQDA
                     AYRYQLQVERKEQVVVGVNDFVVQEGKPDNLLKVDPRVEKEQRKRLAALRKRRDGAAV
                     RAALGKIESACRGKVNVMGPILSA"
BASE COUNT          680 a         1139 c         1275 g          561 t
ORIGIN      
        1 caacatcccg ctgtaggagg catacgtgtt ccggaagatc ctgatcgcga accgcgggga
       61 gatcgccgtc cgggttctcc gcgcctgcaa ggagatgggg atccgcacgg tggcggtctt
      121 ctccgaggtc gaccggaagg cactccatac ccgctacgcc gacgaggcgt actgcatcgg
      181 tcccgcgccg gcgcgggagt cgtacctggt gatcgacaag atcatcgacg tggcgaaaag
      241 gaccggcgcc gaggccattc accccggata cggcttcctc gcggaaaacc cgctgttcgc
      301 ggaccggtgc gagaaggaga agatcaagct gatcggtccc tccgcctatg ccatgcggac
      361 gatgggttcc aagacgctcg cccggaagac cgtccaggcg gcgggcgtcc ccgtcgtccc
      421 cgggaccgtg gagccgatca ccacggaggc ggaggtcttc cgcgtcgcga aggagatcgg
      481 cttcccggtg atgctcaagg cgaccgccgg gggcgggggc aaggggatgc ggctggtgag
      541 ggaggagtcc gagctttcgt cttcccttcg gatggcgaaa tcggaggcga aatccgcctt
      601 cagcgacgac tccgtctaca tcgagaagta catcgagaac ccccgccgtg tggagataca
      661 gctcctcggg gaccggcacg ggaactacgc gcacctgtgc gagcgcgagt gctccatcca
      721 gcggcgccac cagaaggtga tcgaggagtc cccttccgtc atcgtcaccc ccgagatgcg
      781 ggcagcgatg gggaaagtgg cgatcgaggc ggcgcgggcg gtgaagtatg aaggggcggg
      841 aacgtgcgag ttcctcgtgg acgcgaaacg gaacttctac tttcttgaga tgaacacccg
      901 gctccaggtt gagcatccgg tcacggagat ggtgaccggg atcgacatcg tcaaggagca
      961 gatccgtgtg gcggccgggg aagagctctc cttccggcag gaggacgtga ggcagacggg
     1021 ccacgcgatc gagtgccgca tctacgccga ggatccggat agcaacttca tgccgtgccc
     1081 cggcgtggtc acctcgctgc gcatccccgg cggccccgga gtgcgggacg actccggcgt
     1141 gtacgccggg ttcgagatcc cgatctatta cgacccgatc atttccaagc tggttacgtg
     1201 ggggaaggac cggaccgagg cgatcgcgcg gatgaagcgc gcccttggcg tgtacgtggt
     1261 caccggcgtg aagacaaccc tcccgttcca catccgcgtg atgaacaacc ggcacttcat
     1321 cgaagggaac ttcgacacga atttcatcga acaggtgttc ttcaaggagg aagaggcgcg
     1381 gcgcctcgaa aacggggagc tggcgctggt cactgcggcg atccaggcgt tcggggagga
     1441 gcggaggagc gcgttggcgc agcaacccgg ggaggcggga gcacccgtct cgatgtggaa
     1501 gtactccaca cgcccgggca tccggaagat cgggtaggtg cggcgatgaa ctacgtggcg
     1561 acgatcggcg agaaggacgt gaaggtatcc gtcgaggagc tcggcgggtc gaactacaag
     1621 gtgacgatcg acggggtgga gcacgtcgtg gacgcccagc tgatcacgga caacctctgg
     1681 tcggtccttt acggaaacgg gtcgttcgag gtggacgtga cgcggctgcc ggcggaggag
     1741 tacgaagtgc tcatcaaggg cgactgccac aagttcaccc tgatgaacga acagcgccgg
     1801 gcgatgatcc gggcgggcgg caaaggctcc gccgggaagg cgatgctcac ctccccgatg
     1861 cccgggaagg tggtcaagct cctggtcaag gaagggcagg aggtcgaggc cggcgaggga
     1921 gtaatcgtcg tcgaggcgat gaagatggag aacgagctga agtccgccct cgcggggaag
     1981 gtgaaggaga tcttcgtcga ggaaggccag gtcgtcgagt ccggggcgaa gctgctcctc
     2041 gtggaatagg agggcccgat ggcggggaag aacgggaagg gcgggcttcg ggaggcgcgc
     2101 gagcgctggg agcgcgacat cctcgcgccg gcgatgtcga agtcgcggga gcgccgcccc
     2161 cggttcgaga gcacctccgg ggaagaggtc ccccgcctct acacgcccga gcacctcgcg
     2221 tcgttcgatt atctccggga cgcgggctac ccgggcgagt tccccttcac gcgcggcgtc
     2281 cagccgacga tgtaccgcgg ccgcttctgg acgatgcggc agtacgcggg cttcggggac
     2341 gcgaaggagt cgaaccgccg gtaccggtac ctgctcgaca acggccagac gggcctgtcc
     2401 gtggccttcg atcttccgac ccagatgggg tacgactccg acgccctcat gtcgttcggg
     2461 gaggtcggga aggtcggcgt ggcgatcgac tccctcgagg acatggaggt cctgttcgac
     2521 cggatcccgc tcgggaaggt ctccacctcg atgacgatca actcgaccgc cgccatcctc
     2581 ctggcgatgt acatcgccgt cggcgagaag cagggggtgg ccgccgcggc gctgaacggc
     2641 accatccaga acgacatcct gaaggagtac atcgcccggg ggacctacat ctttccgccc
     2701 gccccctcga tgcggatcat caccgacatc ttcgccttct gcaaggacgc cgtcccccgc
     2761 tggaacacga tcagcatctc cggctatcac atccgcgagg cggggtcgac ggctgcgcag
     2821 gaagtcgcct tcaccctcgc gaacgggatc gcctatgtgc aggcggcgat cgacgccggg
     2881 atgtcggtcg actccttcgc gtcgcgcctg gccttcttct tcaatgccca caacaacttc
     2941 ctcgaggagg tcgccaagtt ccgggccgcg cggcggctgt gggccgggat catgcgggag
     3001 cggttcgggg cgaaggatcc ccgctcctgg atgctccggt tccacacgca gaccgccggg
     3061 tcgtccctca ccgcgcaaca gcccgacaac aacatcgtcc gcgtgacgat ccaggcgctt
     3121 tcggccgtcc tcggtggcac ccagtcgctg cacaccaact cgcgcgacga ggcgctgtcc
     3181 cttcccacgg aggcgtcggt gcgcattgcc ctccgcacgc agcagctgat cgcccacgag
     3241 agcggggtgg ccgaaacggt cgatcccctc ggcgggtcct ggtgcgtcga ggccctcacc
     3301 tcggggattg agcagcaggc gcgggagtac atcgcccgga tcgaccggat gggcggcgtc
     3361 attccggcct tggaatccgg ctacatccag aaagagatcc aggacgcggc gtaccggtac
     3421 cagctgcagg tcgagcggaa ggagcaggtc gtcgtggggg tgaacgactt cgtcgtgcag
     3481 gaaggaaagc ccgacaacct cctcaaggtc gatccccggg tcgaaaagga acagcgcaag
     3541 cgcctcgcgg ccctccggaa gcggcgcgac ggcgccgcgg tccgcgccgc cctcggcaaa
     3601 atcgagtccg cctgtcgggg aaaggtgaac gtaatgggac cgattctttc cgccg
//
LOCUS       DASVJL010000322         4641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1646939, whole genome shotgun sequence.
ACCESSION   DASVJL010000322 DASVJL010000000
VERSION     DASVJL010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646939"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /gene="pnp"
                     /locus_tag="VF357_06785"
     CDS             complement(<1..1175)
                     /gene="pnp"
                     /locus_tag="VF357_06785"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX7523889.1"
                     /translation="MESGRWARQAGGAVVVRYGESVVLVTACLSENPRPGIDFLPLVV
                     DYVEKTSAVGKIPGGFFKREGRLSEFEILTSRMIDRPIRPLFPKGFYNEIQIVATVLS
                     ADKENDTGILAMIGASAALSISEIPFTGPIAGARVGRIDGKLVVNPILPDFERSDLNI
                     FVAGSRDAILMVEGESNEVSEEEVLDAILFAHRSLVPILDLQDRMAREIGKEKRVFAK
                     KALPEEDLRKIASIAEAPFAEAYAIREKQDRRKRIEAIGESVRAAFPDEERAEKGALI
                     SDALKSLEKKYVRGTILREKKRIDGRGLTEVRAISCEVGVLPRTHGSAVFTRGETQAL
                     VMATLGTSQDEQRIDSIMGDTTKSFMLHYNFPPFSVGEVKMLRGPGRREVGHGALAER
                     "
     gene            complement(1303..1572)
                     /gene="rpsO"
                     /locus_tag="VF357_06790"
     CDS             complement(1303..1572)
                     /gene="rpsO"
                     /locus_tag="VF357_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX7523890.1"
                     /translation="MSLVTEKKSDIIGKFRVHDTDTGSPEVQVALLTERINMITDHLK
                     IHSKDFSTRRGLLKLVGQRRRLLDYLKSVESMRYKALLETLGLRK"
     gene            complement(1662..2552)
                     /gene="truB"
                     /locus_tag="VF357_06795"
     CDS             complement(1662..2552)
                     /gene="truB"
                     /locus_tag="VF357_06795"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX7523891.1"
                     /translation="MTAGVLVVDKPSGITSFDAVQAVGRILKERKCGHAGTLDPMATG
                     VLPVCVGAATKIAGYLTEEEKEYEATFAFGVATDTGDATGKPVEERPGATATEGAVAE
                     TLAALVGTFEQVPPSYSAVKVGGVRSYTLARKGMEVPLAARRVTVREARLLFIGPDRF
                     RVFLAVSKGFYVRSLPRDLGARLGIPLTVSELRRTRVGPFRAELSVTLDALRERARNV
                     DAASLLIPIADALGRFPHWEVPAEAISQVRNGRLSGSWLVERAATERGDLALLVTSRR
                     EPLAIVGRDPTGLWKIVRGI"
     gene            complement(2549..3511)
                     /locus_tag="VF357_06800"
     CDS             complement(2549..3511)
                     /locus_tag="VF357_06800"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX7523892.1"
                     /translation="MRGDLSAICRVLREKDRFLIACHENPEGDAIGSELALALALRKM
                     GKTATVLNADPVPGNLLFLPGAGTVVFAEDGSKYDVAVVVDCGSPDRTGSVAQELRKC
                     PLLVNIDHHRTNGDLGELALVDPDAAATGLLIHRVLSAMGYEIGLEVATNIYVAVLTD
                     TGSFHYGSSSPEAFEVAGEMVRRGVDPWAVAEQVYETQSALRLRLLGRVLASLEVSAD
                     GRVACITTKREDLREFAAGKDALEGFINYPRSIVGVEVAVSLREEEGDVFRVSFRSKG
                     RVDVSAVAARFSGGGHRNAAGCTVPGPLADAKKRVLEALAAVLS"
     gene            complement(3501..3872)
                     /gene="rbfA"
                     /locus_tag="VF357_06805"
     CDS             complement(3501..3872)
                     /gene="rbfA"
                     /locus_tag="VF357_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX7523893.1"
                     /translation="MKGRGDRPARVSERIREELSLLLLRKVNDPGLAPVTVTDVSVTK
                     DLRIAHVNYSALVSKEERPAVAKALRRSSGYLRRELGHLLGLRYAPELQFHYDDSFDR
                     GARIDAILRDIPEGKEGPDEG"
     gene            complement(3869..4168)
                     /locus_tag="VF357_06810"
     CDS             complement(3869..4168)
                     /locus_tag="VF357_06810"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX7523894.1"
                     /translation="MAVLIAELLFPDAASLKDKRRRLAGLVARIRANYPVSVAEVGGQ
                     DLWQRGTVGAALVTTDGRLARSMFDRITGGIGRDGEIELLSSRVEFFHPEGSEAE"
     gene            complement(4197..>4641)
                     /locus_tag="VF357_06815"
     CDS             complement(4197..>4641)
                     /locus_tag="VF357_06815"
                     /inference="COORDINATES: protein motif:HMM:NF015126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX7523895.1"
                     /translation="IGFNVRPEPKAAVLAEREGVDIRLYSVIYDVVDDVKKAMEGLLD
                     PTLKEVVLGRAEVRNMFHISKIGTIAGCSVLSGKIARGANIRLLRDSVVVFEGKLSSL
                     KRFKDDVKEVAEGYECGLGIDGYNDLQVGDHVEAFVIEKIAGTLA"
BASE COUNT          762 a         1595 c         1480 g          804 t
ORIGIN      
        1 cgctcggcga gcgccccgtg cccgacctcg cggcggccgg gaccgcgcag catcttgacc
       61 tctccgacgc tgaagggcgg gaagttgtaa tgaagcatga acgacttggt ggtgtccccc
      121 atgatcgagt cgatccgctg ctcgtcctgc gacgtcccga gggtggccat gaccaaggcc
      181 tgcgtctcgc cccgggtgaa gacggcggag ccgtgcgtgc gggggagcac cccaacctcg
      241 caggagatgg cgcggacttc cgtcagccca cggccgtcga tccgcttctt ctcccggagg
      301 atcgtcccgc ggacatactt cttttccagg cttttcaggg cgtcggagat cagcgccccc
      361 ttttccgcgc gctcctcgtc ggggaaggcg gcgcgaaccg actcgccgat cgcctcgatc
      421 cgcttgcgcc ggtcctgctt ctcccggatg gcgtacgctt ccgcgaaagg agcctcggcg
      481 atcgacgcga tcttccgcag gtcttcctcc gggagggcct tcttcgcgaa gacccgcttc
      541 tcctttccga tttcccgggc catccggtcc tggagatcga ggatcggcac cagcgaccgg
      601 tgcgcgaaga ggatcgcgtc gagcacctcc tcctcggaca cttcgttcga ctctccctcg
      661 accatcagga tcgcgtcgcg gcttccggcg acgaagatgt tcaggtcgct ccgctcgaag
      721 tcgggaagaa tggggttgac caccagtttt ccgtcgatgc ggccgacccg cgccccggcg
      781 atcggcccgg tgaaggggat ctcggagatc gagagcgcgg ccgaggcgcc gatcatggcc
      841 aggatcccgg tgtcgttttc cttgtccgcg gagagaacgg ttgcgacgat ctggatctcg
      901 ttgtagaacc ctttcgggaa gaggggacgg atggggcggt cgatcatccg ggaggtgagg
      961 atctcgaatt cggaaagcct cccttcgcgc ttgaaaaagc cgccggggat ctttccgacc
     1021 gcggaggtct tctccacgta gtcgacgacc agcgggagga agtcgatgcc cgggcggggg
     1081 ttctcggaga ggcaggcggt gaccagcacg accgattccc cgtagcggac gaccaccgcg
     1141 ccgccggcct gacgggccca acggcctgat tccatggaca ggttccgtcc ggatacttca
     1201 atttcgtaca cgtttcccac agtgatgggt tcccttctcg tagcggcccc cccgtgggga
     1261 ccgtggattg tgttggcgga aatttctgga gcgatgccgg gattacttgc gaaggccgag
     1321 cgtctccaga agggccttgt accgcatcga ttccaccgac ttcaggtaat ccagcaggcg
     1381 ccgccgttgc ccgaccaact tgagcaggcc acgtcgcgtg ctgaagtcct tggagtgaat
     1441 tttgaggtgg tcggtgatca tgttgatccg ctccgtcagg agcgcgacct ggacctcggg
     1501 ggaaccggtg tccgtatcgt gcacacggaa ctttccgatg atgtcgcttt ttttctcggt
     1561 gaccagactc atgatctttt gcctttctcc tttcgttgcg tcgagaatgt tccgttttta
     1621 ttgaggaatt aatttatacc atatctgatt ccgtatgtaa atcagatgcc acgaacgatt
     1681 ttccaaagtc ccgtcggatc ccgaccgacg atcgccagcg gctcccgccg cgacgtcacg
     1741 agcagcgcga gatccccccg ctctgtcgcg gcccgctcga ccagccacga gccggaaagc
     1801 cgtccgttgc gcacctggga gatcgcctct gccggcactt cccagtgagg gaaccgcccg
     1861 agtgcatccg cgatcgggat cagcagggag gctgcgtcga cgttccgtgc ccgttcgcgc
     1921 agcgcgtcca gcgtcaccga cagctccgca cggaacggtc ctacccgggt gcgccgcagc
     1981 tccgagaccg tcaacggaat cccgagacgc gctcccagat cgcgcggcag cgaccggacg
     2041 taaaacccct tcgacacggc gaggaacacc cgaaaccggt ccggaccgat aaagaggagc
     2101 cgcgcctcgc ggaccgtgac ccggcgggcc gcgagaggaa cctccatccc cttccgcgcg
     2161 agcgtgtagg agcgcacgcc gcccaccttg accgcggagt acgacggggg gacctgctcg
     2221 aaggtcccca ccagagccgc cagcgtctcc gccacggcgc cttccgtcgc cgtggccccg
     2281 ggacgctcct ccacgggctt ccccgtggcg tcgccggtgt ccgtcgcgac gccgaacgcg
     2341 aacgtcgcct cgtactcctt ctcttcttcc gtgagatagc ccgcgatctt cgtggcggct
     2401 ccgacgcaca ccggcaggac gccggtggcc atcgggtcga gggtgccggc gtgcccgcac
     2461 ttccgctcct tgaggatccg acccacggcc tggaccgcgt cgaaggaggt gatccccgaa
     2521 ggcttgtcga cgaccagcac ccccgccgtc acgacaacac ggccgcgagc gcctcgagca
     2581 ccctcttctt cgcgtcggcg aggggccccg ggacggtgca tccggcggca ttgcggtgcc
     2641 ctcccccgct gaaccgggcg gcgacggcgg agacgtcgac gcgccccttg gagcggaagc
     2701 tcacgcggaa gacgtcccct tcctcctcgc ggagggagac ggcgacttcg acaccgacga
     2761 tggagcgggg gtagttgatg aatccctcga gggcgtcctt cccggcggcg aactcccgca
     2821 ggtcttcccg cttcgtggtg atgcaggcga cccttccgtc ggcggaaact tcgagggaag
     2881 cgagcacccg gccgagcaac cggagacgaa gggcgctctg cgtctcgtat acctgctccg
     2941 ccacggccca cggatcgact ccccgccgga ccatctcccc ggctacctcg aacgcttccg
     3001 gcgaagagct cccgtaatgg aacgaccctg tatccgtgag gacggcgacg tagatgttgg
     3061 tcgccacctc gagtccgatc tcgtacccca tcgccgagag gacccggtgg atgaggagcc
     3121 ccgtagcggc ggcgtcggga tcgaccaggg cgagttcccc gagatcgccg ttcgtccgat
     3181 ggtggtcgat gttgacgaga agggggcact tccgcaactc ctgggcgacg ctccccgtcc
     3241 ggtccggaga gccgcaatcc accacgaccg ccacgtcgta cttcgatccg tcctccgcga
     3301 agacgaccgt gccggccccc ggaaggaaga ggaggttgcc gggaaccggg tcggcgttca
     3361 gcaccgtcgc ggtctttccc attttccgca gggcgagggc gagcgccagc tcggacccga
     3421 tcgcgtcccc ttccggattt tcgtggcagg cgatgaggaa acggtccttt tcccgcagga
     3481 cccggcagat cgcggagagg tcacccctca tccggacctt ccttcccctc cgggatatcg
     3541 cggaggatcg cgtcgatccg ggcgccgcgg tcgaacgagt cgtcgtagtg gaactgcagt
     3601 tcgggggcgt accgcaggcc gaggagatgg ccgagctccc gccgcaggta accggaggac
     3661 cgacggagcg ccttggccac ggcggggcgc tcctccttcg acacgagagc ggagtaattc
     3721 acgtgggcga tccgaaggtc cttcgtcacg gaaacatcgg tgacggtgac cggcgccagc
     3781 cccgggtcgt tcaccttccg cagcaggagg agggagagct cttcccggat cctctcgctg
     3841 acacgcgcgg ggcggtcgcc gcgacccttc attccgcttc gctcccctcg gggtggaaga
     3901 actcgacgcg ggaggagaga agctcaatct ccccgtcacg gccgattccg ccggtgatcc
     3961 ggtcgaacat cgaccgggcg agccgcccgt cggtggtgac cagcgccgcg ccgacggtcc
     4021 cccgctgcca gagatcctgt ccccccacct cggcgaccga tacggggtag ttcgcgcgga
     4081 tccgcgcgac gaggcccgcg aggcgccgcc gcttgtcctt gagggacgcc gcgtccggga
     4141 agagaagctc cgcgatcagg accgccacca gcaacgcatt cccccgcctg aaaacgtcac
     4201 gccagcgtgc cggcgatctt ctcgatcacg aacgcctcga catggtcgcc cacctggagg
     4261 tcgttgtacc cgtcgatccc gaggccgcac tcgtaccctt ccgcgacctc cttgacgtcg
     4321 tccttgaacc gcttcaggga ggagagtttt ccctcgaaca cgaccacgct gtcccgaagg
     4381 aggcggatgt tcgcgccgcg ggcgatcttc cccgacagga cgctgcaacc ggcgatcgtg
     4441 ccgatcttgg agatgtggaa catgttgcgc acctcggccc gccccaggac gacctcctta
     4501 agggtgggat cgaggagtcc ctccatcgcc tttttcacgt cgtccaccac gtcgtagatg
     4561 acggagtaga gccggatgtc gaccccctcg cgttccgcga gcacggccgc cttcggttcg
     4621 ggccggacgt tgaacccgat g
//
LOCUS       DASVJL010000323         6173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_522663, whole genome shotgun sequence.
ACCESSION   DASVJL010000323 DASVJL010000000
VERSION     DASVJL010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522663"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1026)
                     /locus_tag="VF357_06820"
     CDS             complement(154..1026)
                     /locus_tag="VF357_06820"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEX7523896.1"
                     /translation="MAGAFLAGLLLVGVILIRTPEAERSTADNLADLPAVIPTSSVAS
                     DNGGAGPAPGPRLAIVVDGLGYDPVRDAEWLDFPERITASVLPYGPSTKSFAASARTH
                     GFGVILHVPMESEGGVADRTEPFLLRRGMTQEEIADRFSRMADDVPQANGASNHMGSA
                     FTSDLAAMTSFAQALKGKGFFFVDSVTSAGTVASMAMEDAGVRAMRRDVFLDDDGRPE
                     EMRRQWTAAVALAKERGDAILLCHARRETRKALLELIPQLRAAGIRPVTVEELLAQPS
                     RTAHEAGGSPERRP"
     gene            complement(1101..2498)
                     /locus_tag="VF357_06825"
     CDS             complement(1101..2498)
                     /locus_tag="VF357_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7523897.1"
                     /translation="MEPTGAASRKTAGKKWLGTVLIVAVFVGGFVVGDLTTSRHAAQA
                     NVAYSKLKLFGDVLSIIQSSYVEEVNIDNLVRGAVNGMVQTLDPHSSYLTPEMLKQVE
                     VETKGVFGGLGIEIGMKDGFLTVIAPIEDTPAARAGLLAGDKIVKIENESTKNMNVMD
                     AVKRLRGEPGSKVTITVARESLPEPKAYILTRDIIKIKSVKTKSMGDGIGYIRLTQFQ
                     QDSHQEVNRALQEFLKEKGGLKGLILDLRNDPGGLLDQAVRIADEFIESGLIVYTDGR
                     VEAQKTKYVAHKEGTYTGFPIVVLVNAGSASASEIVAGALQDHGRAIIIGQRTFGKAS
                     VQTIIPLEDGSALRLTTARYYTPNGRSIQAKGIEPDIVVPDGREPPDGHAGMLREMDI
                     ERHLKGDGEEPPAPVVVPKAGKKEDQGGVKKETPAEPEIRKEDAKDPQLDRAVELLKG
                     WEIFKSRFIDRQKAS"
     gene            complement(2514..3737)
                     /locus_tag="VF357_06830"
     CDS             complement(2514..3737)
                     /locus_tag="VF357_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX7523898.1"
                     /translation="MIWGIRRILSVLLAAAVSLCILFPPAPAVARDVAADLRKERGLL
                     LEMKGREEKTAQELTEALRKEKRSKGRVGELQELLTRQRILLSRIDRKVSVLNDRLEG
                     AEKEVRELEAAHGQARTDLQRAEVVAFAVQKAGAGDPRHPSARDRERHFMRVYLVANA
                     EGVARITRARERKEEELSGLERQVAVTERKMAMEKKVGDTLLSRQEGERRILADIERD
                     KKRKEKELKSLRAKIARMESLVSRVERQVKERERRARKAAAGGSAAREPAETPRRFAS
                     LSGGLSAPLAGKVITRFGRQHDTTFDVTIENRGVEIEAPSGASVKSVGQGEVAFAGAV
                     SGFGNVLIVQHGGGLFSVYGRLETFLVKQGEAVTKGTVVGRLPGSPSGKSVLYLELRA
                     GGTAIDPTSAIPLDS"
     gene            complement(3734..4624)
                     /locus_tag="VF357_06835"
     CDS             complement(3734..4624)
                     /locus_tag="VF357_06835"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX7523899.1"
                     /translation="MSEQPPVWWIDWRLSRGPLVRETIGRFLFFCLLGFALLSLLLSG
                     GASRFLADRYAITAVLRAEVPSAEAEGLARKVAALPPVRSAAYQDPEAAWKEFLRAYP
                     GLDPLRDAGGNPMPGYIEIRFRSDRLTGADVDLVVSALRSVPLVEQVLAGEEWLPRLL
                     RAGRFAFRVGWGIFGAFLAGFFLVGRLQERARAVALAGDFAFLAERGVPAGRMAFFRA
                     AAAAVSGFLLAAAGTAAGGGALFLSLRKFPFLEGVVGPPSDLLLPRTVAAAVLFSCVA
                     ALLSAAASLLGWRAARSGRK"
     gene            complement(4621..5316)
                     /locus_tag="VF357_06840"
     CDS             complement(4621..5316)
                     /locus_tag="VF357_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7523900.1"
                     /translation="MIEGFRLGVYSRGTTLWDGLDFAFGDAAAWFVAGPPSSGKTLLL
                     SILRGERRPDAGDVLVSGESLYRGNAALARAWGASCGHVPEQTICDARLTVGDLFRRS
                     ALAGGGVRGSDRKDRTDRLLEMVGLPGALGWRVAELSISERVRACLAAELLRGPKVLF
                     CDGVVAGAGIPFREMLWGLFRALAREGNTVVLAERTIPERWALAAGDAEPVGPFRLYR
                     LPVPGAEAKGDPG"
     gene            complement(5313..5609)
                     /locus_tag="VF357_06845"
     CDS             complement(5313..5609)
                     /locus_tag="VF357_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523901.1"
                     /translation="MGGPQMRMLSGFNHNIRFRGKVYHVQTEDGGKDNPQIITHAFQG
                     GAILDSVRTSYADLLERPDWQADLKDRMKAQHLEEIRRLMAGDIVPPEGAAGDR"
     gene            complement(5656..>6173)
                     /locus_tag="VF357_06850"
     CDS             complement(5656..>6173)
                     /locus_tag="VF357_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX7523902.1"
                     /translation="GMVDPCVRRAAALPEKRTIGVIGTLGTVRSGAYEEALRHSVPSA
                     RVRSIPCPLLVSLAEEGWTDNAITRAVIAEYLAPFLEDPPDALILGCTHYPVLKGPIR
                     EYLGGDPVLIDSAEEAARVVDILLSETQVRREGTPGAVEFLVTDDPERFARVGKGFFG
                     RELPEVGRVAL"
BASE COUNT          987 a         2258 c         1935 g          993 t
ORIGIN      
        1 gacccgtacg gtccggaagg acgactcgag gagccgcttg atcttcccgg taagctcact
       61 gacggaaaaa acgtccccgg agggcgatgt accaaagaga tccagttcca ctaaattctc
      121 catccgggag accgcttctc cgcaggaccg tacttacggt ctccgctcag gggacccccc
      181 tgcttcgtgc gcggttcttg agggctgcgc cagaagctct tcaacggtca cggggcggat
      241 ccctgccgcg cgcaactgcg ggatcagctc aagcaaggcc ttgcgcgtct cccggcgggc
      301 gtggcacagg agaatcgcgt cgccccgctc cttcgccagg gcgacggcgg cggtccactg
      361 acgccgcatc tcctcgggcc ggccgtcgtc gtcgaggaag acgtcgcgcc gcatcgcgcg
      421 gacacccgcg tcctccatgg ccatcgacgc gacggtgccc gccgaggtca cgctgtcgac
      481 gaagaagaac ccttttccct tgagcgcctg cgcgaaggag gtcatcgcgg cgaggtcgga
      541 ggtgaacgcc gaccccatgt ggttgctcgc cccgttcgcc tggggaacat cgtcggccat
      601 ccgggagaac cggtccgcga tctcctcctg cgtcatcccc cggcgaagga ggaacggctc
      661 ggttcggtcg gcgacgccgc cttccgactc catcgggacg tggaggatca cgccgaagcc
      721 gtgggttcgg gcggaagccg cgaacgattt ggtagagggg ccgtacggta aaacggatgc
      781 ggtgatccgc tcggggaagt cgagccattc ggcgtcccgc accggatcgt acccaaggcc
      841 gtcgacgacg atcgcaagcc gggggcccgg agccgggccg gcccccccgt tgtccgatgc
      901 cacagaggac gttgggatca cggcggggag gtcggcgagg ttatccgccg tcgaccgctc
      961 cgcctcgggc gtccggatca ggatcacacc caccagtagc aggccggcga ggaacgcccc
     1021 ggccatcagg gctgcccacc cccaccgctt ccttccgctg cccgtccccc cggcggctgt
     1081 ccccggcata ttccgcccgg tcaggacgcc ttctgccggt cgatgaaccg ggacttgaag
     1141 atctcccacc ccttcagaag ctcgaccgct cggtcgagtt ggggatcctt cgcgtcctct
     1201 ttgcggatct ccggctcggc cggggtttcc ttcttcacgc cgccctggtc ctcctttttc
     1261 cccgccttcg gaacgacgac cggcgccggg ggttcctcgc catccccctt aaggtgccgc
     1321 tcgatgtcca tctcccggag catccccgcg tgcccgtcgg gcggctccct gccgtccggg
     1381 acgacgatgt cgggctcgat ccccttcgcc tggatcgacc gcccgttggg ggtgtagtac
     1441 cgcgccgtcg tcagccggag cgcggagccg tcctccagcg gaatgatcgt ctggaccgac
     1501 gccttgccga aggtgcgctg gccgatgatg atcgcccggc cgtgatcctg cagcgcgccg
     1561 gcgacgatct cggaggcgga ggcggacccg gcgttcacca gcacgacgat cgggaagccg
     1621 gtgtacgtcc cctccttgtg ggcgacatac ttcgtcttct gggcctcgac ccggccgtcg
     1681 gtgtagacga tgagccccga ttcgatgaac tcgtccgcga tccggaccgc ctggtcgagc
     1741 aacccgcccg ggtcgttccg caggtcgagg atgagccctt tcagcccccc cttctctttc
     1801 aggaattcct gcagggcgcg gttgacctcc tggtgggagt cctgctggaa ctgcgtcaga
     1861 cggatgtaac cgatcccgtc ccccatcgat ttggtcttga cgctcttgat cttgatgatg
     1921 tcgcgcgtga ggatgtaggc cttcggctcg ggcagcgatt cccgcgccac cgtgatcgtc
     1981 accttgctcc ccggctctcc ccggagccgc ttcaccgcgt ccatcacgtt catgttcttt
     2041 gtcgactcgt tctcgatctt cacgatcttg tccccggcca gcaggcccgc acgggccgcg
     2101 ggggtgtcct cgatcggcgc gatcaccgtg aggaagccgt ccttcatccc gatctcgatc
     2161 cccaggcccc cgaacactcc cttcgtctcg acctcgacct gcttcagcat ttccggcgtg
     2221 aggtaggaac tgtgcggatc gagagtctgg accatcccgt tcacggcccc ccggacgagg
     2281 ttgtcgatgt tcacctcctc gacgtagctg ctctggatga tcgagaggac gtcgccgaaa
     2341 agcttcagct tgctgtaggc gacgttcgcc tgcgcggcat gccgggaggt cgtgaggtct
     2401 cccaccacga accctccgac gaacaccgcg acgatcagga cggttcccag ccacttcttc
     2461 cccgccgtct tacgggatgc ggctcctgtt ggctccatgc gcttgtaggc tccttaactg
     2521 tcgagtggaa tggcggaggt cggatcgatg gcggtgcctc ccgcccgcag ttccaagtat
     2581 agcaccgatt taccggatgg cgatcccggc agccgcccca ccaccgtccc ttttgttacg
     2641 gcttcgccct gctttacgag gaatgtttcc aggcggccgt acacggaaaa gagtccgccg
     2701 ccgtgctgca cgatcagcac gttcccgaat ccagagacgg cccccgcgaa cgcgacctcc
     2761 ccctggccga ccgacttcac ggacgccccc gaaggagcct cgatctccac cccccggttc
     2821 tcgatcgtca cgtcgaaggt cgtgtcgtgc tggcgtccga agcgggtaat gaccttcccc
     2881 gccagcggcg ccgaaagccc tcccgaaagc gacgcgaacc gtcgcggggt ctccgcgggc
     2941 tccctggccg ccgatccccc cgccgccgcc ttccgcgcgc gccgttcccg ctccttcact
     3001 tgccgctcga cccgggagac gagggactcc atgcgggcga tctttgcgcg aagggatttc
     3061 aactccttct ccttgcgctt cttatcccgc tcgatgtcgg cgaggatccg gcgctccccc
     3121 tcctgccggg agaggagcgt atcgcccacc ttcttctcca tggccatctt cctctcggtg
     3181 acggcgacct gccgctcgag cccggaaagt tcctcctcct tccgctcccg ggcccgggtg
     3241 atccgggcga ctccctccgc gttcgccacg aggtagacgc gcatgaagtg ccgttcccga
     3301 tcccgggcgg acgggtgccg gggatccccc gctccggcct tttgcacggc gaaggcgacg
     3361 acctccgccc gctggaggtc ggtccgggcc tgcccatgcg ccgcctcgag ttcccgaacc
     3421 tccttctccg ccccctccaa ccggtcgttc aagaccgaga ccttccggtc gatgcgggaa
     3481 agcaggattc tctggcgcgt aaggagctcc tgcagttcgc cgacgcgccc cttcgaacgt
     3541 ttctccttcc ggagcgcctc ggtcagctcc tgggccgtct tctcctcccg ccccttcatc
     3601 tcgaggagca ggcctctctc ctttcgaaga tcggccgcga catcccgcgc caccgccggc
     3661 gccggcggga agaggataca aagcgacacc gccgcggcga gcaggacaga gaggatccgg
     3721 cggattcccc agatcatttc cggccggagc gggccgccct ccatccgagg agggacgcag
     3781 ccgccgagag cagggccgcg acgcaagaga agaggacggc cgccgcaacc gtccggggaa
     3841 gaaggaggtc tgaggggggg ccgaccaccc cttcgagaaa cggaaacttc cgaagggaga
     3901 ggaacagcgc cccgccgccc gccgccgttc cggccgcggc gaggagaaat ccggaaaccg
     3961 ccgccgccgc cgcgcggaaa aaggccatcc ggcccgcggg gacaccccgt tccgcgagaa
     4021 acgcaaaatc acccgcgagg gcgaccgcgc gcgcccgctc ctggagacgg ccgacaagga
     4081 agaatccggc gaggaaagcg ccgaagatcc cccagcccac ccggaatgcg aaacggccgg
     4141 cgcgcagcag ccggggaagc cactcctccc ccgcgagaac ctgttcgacg agaggaacgg
     4201 accggagggc ggagacgacg aggtcgacat cggcccccgt caaccgatcg gaacggaacc
     4261 ggatctcgat gtaccccggc atcgggttcc cgccggcgtc gcgcagcgga tccagacccg
     4321 ggtacgcccg caggaactct ttccacgccg cctccgggtc ctggtacgcc gcggagcgca
     4381 ccgggggaag ggcggcgact tttcgcgcca gtccctccgc ttcggcggaa gggacctccg
     4441 cgcgcagcac cgcggtgatg gcgtaccggt cggcaaggaa acgactcgcc cctcccgaca
     4501 ggagcaggga aagtagcgcg aaaccgagga ggcagaaaaa gaggaaccgc ccgatcgtct
     4561 cccggacgag agggccccgg gaaagacgcc agtcgatcca ccacacggga ggctgttcac
     4621 tcaaccggga tctcctttcg cttccgctcc cggtaccgga aggcggtaca accggaaagg
     4681 cccgacgggc tccgcgtcgc ccgcggccaa ggcccaccgt tcggggatcg ttcgttccgc
     4741 gagaacgacc gtgttccctt cccgggccag ggcgcggaac agtccccaga gcatctcccg
     4801 gaacggaatc cccgcgccgg ccaccacgcc gtcgcagaac aggacctttg gcccgcgcag
     4861 cagctccgcg gccaggcagg cccgaactct ttcggagatc gacagctccg cgaccctcca
     4921 ccccaacgcg cccggcaggc cgaccatctc gaggagccga tccgtgcggt ccttccggtc
     4981 gcttccccgc acgccgcccc cggcaagggc ggagcggcga aagaggtccc ccaccgtcag
     5041 acgcgcgtcg cagatcgttt gttccgggac gtggccgcag gaagcgcccc aggcgcgggc
     5101 aagggcggcg ttcccgcggt agagggactc cccggagacg aggacgtccc cggcatccgg
     5161 cctgcgctct ccgcggagga tggagaggag caacgtcttc cccgacgacg gcggtccggc
     5221 gacgaaccac gcggcggcgt ctccgaacgc aaagtcgagt ccgtcccaga gggttgtccc
     5281 ccgggagtag acccccagtc ggaacccctc gatcatcggt ccccggcggc cccttcggga
     5341 ggcacgatgt ccccggccat cagacgccgg atctcctcga ggtgctgcgc cttcatccgg
     5401 tccttgaggt cggcctgcca gtccggccgc tcgagcaggt ccgcgtagga cgtgcggacg
     5461 ctgtcgagga tcgccccgcc ctggaacgca tgagtgatga tctgcggatt gtccttcccc
     5521 ccgtcctccg tctgaacatg gtagaccttt ccccggaacc ggatgttgtg gttgaatccc
     5581 gagagcattc tcatctgcgg tccgcccatc gccccttcgc ctctcccctt tcagttcgtg
     5641 ccgtaccgga acccgctaca gggccacacg gcccacttcg ggcagctccc ggccgaaaaa
     5701 tcccttcccc acccgcgcga accgctccgg gtcgtccgtc acgaggaact cgaccgcccc
     5761 cggggtccct tcccggcgga cctgcgtctc ggagagaagg atgtcgacga cccgcgcggc
     5821 ctcctcggcg gagtcgatca ggacgggatc gcctccaagg tattcccgga tcggcccctt
     5881 cagcacgggg taatgcgtgc atccgaggat cagcgcgtcg ggcgggtctt ccaggaaagg
     5941 cgcgaggtat tccgcgatca cggcgcgcgt gatcgcgttg tcggtccacc cctcctccgc
     6001 cagcgagacg agcagcggac acgggatcga ccggacccgg gccgagggaa cggagtgccg
     6061 cagcgcctct tcgtacgctc ctgaacgaac cgtcccgagc gtcccgatga cgccgatcgt
     6121 ccgcttttct ggcagggcgg cggcgcgccg aacgcacggg tccaccatcc cca
//
LOCUS       DASVJL010000324         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1742663, whole genome shotgun sequence.
ACCESSION   DASVJL010000324 DASVJL010000000
VERSION     DASVJL010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742663"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VF357_06855"
     CDS             <1..751
                     /locus_tag="VF357_06855"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX7523903.1"
                     /translation="AFGTHKGNLPHEDLIGKEDGGRAWTAMGSEYRAFRPTYMQFIMN
                     QKRHAQIIYPKDTGTILMWADIFPGATVIEAGIGWGALTIKLLEAVGPTGKVVSYEIR
                     EDFAESGASTVRRYLGECENHEVKLRDIYLGIDEREVDRIVLDLPEPWQAVPHAREAL
                     APGGIVLSYLPSAIQVKQLCDRYLEEGGFAEPETFEVILRPWHVKGNSVRPVQWMFSH
                     SAFLVVTRKITVTPEPSPPEPGRSKKFPAEN"
     gene            832..1584
                     /locus_tag="VF357_06860"
     CDS             832..1584
                     /locus_tag="VF357_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX7523904.1"
                     /translation="MKILVAIKPVPNPDEKVKIKRDGSGIVLDNIKMVVNPFCEIAVE
                     EALRIREKLAGEVVVVTVGPKESEQQVRTALAMGADRAVLVEAGADLDSLAVAKVLAK
                     VVSDEKAELVLMGKQSVDDDNNQVGQILAAVLGWPQATFASKIEFGADNKKANVTREV
                     DGGLETIEVALPAVVTTDLRLNEPRYASLPGIMKAKKKELKVVPVASLGVDTTPKVKL
                     ISFAPPKLRAGGGRVADAAELVSKLKSEAKVL"
     gene            1595..>1915
                     /locus_tag="VF357_06865"
     CDS             1595..>1915
                     /locus_tag="VF357_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523905.1"
                     /translation="MADILVVVEHHEGVFKKNTLSVVSAAKALAGLSGGEVDALVLGD
                     GAAAAVADVAAGTGVRKVLLGEGAAFAKYLAATYAGAVAQVVKAKGYGAVFAPASTFG
                     KDFMP"
BASE COUNT          355 a          555 c          712 g          293 t
ORIGIN      
        1 ggcgttcggc acgcacaagg ggaatctgcc gcacgaggac ctgatcggca aggaggacgg
       61 cggccgcgcg tggaccgcga tggggagcga gtaccgcgcc ttccgcccca cctacatgca
      121 gttcatcatg aaccagaagc ggcacgccca gatcatctac ccgaaggaca cggggacgat
      181 cctgatgtgg gcggacatct tccccggggc cacggtgatc gaggcgggaa tcgggtgggg
      241 ggccctcacc atcaagctgc tggaggccgt cggcccgacg ggaaaagtgg tctcctacga
      301 gatccgggaa gatttcgcgg aaagcggcgc gagcaccgtc cggcgctacc tcggcgagtg
      361 cgaaaaccac gaagtgaagc tgcgggacat ctacctcggg atcgacgagc gggaggtgga
      421 ccggatcgtc ctcgacctgc cggagccgtg gcaggctgtc ccccacgcgc gggaggcgct
      481 cgccccggga ggaatcgtcc tttcctacct cccctccgcg atccaggtga agcagttgtg
      541 cgaccgatac ctggaggaag gcggattcgc ggagccggag acgttcgagg tgatcctgcg
      601 cccctggcac gtgaaaggga actccgtccg acccgtccag tggatgttct cccactccgc
      661 cttcctggtc gtgacgcgga agatcaccgt aaccccggag ccatcccccc cggaaccggg
      721 acgttccaag aagttccccg ccgagaattg aaccatcgtt cattcttgcg tttggcatgc
      781 gttcccgata tgttatgacg ttcgaagcgt tgaatccctg ggaggtgcgg cgtgaagatc
      841 cttgtcgcca tcaagccggt ccccaacccc gatgagaagg tcaagatcaa gagggacggg
      901 agcgggatcg tcctggacaa catcaagatg gtcgtgaacc cgttctgcga gatcgcggtc
      961 gaggaagcgc tgcggatccg ggagaagctg gccggcgaag tggtcgtcgt gaccgtcggc
     1021 ccgaaggaga gcgagcagca ggtgcgcacg gcgctggcga tgggcgcgga ccgcgcggtg
     1081 ctggtcgaag cgggggccga cctcgactcc ctggcggtgg cgaaggtgct ggcgaaggtc
     1141 gtctccgacg aaaaggccga gctcgtgctg atggggaagc agtcggtcga cgacgacaac
     1201 aaccaggtgg ggcagatcct ggcggcggtg ctcgggtggc cgcaggcgac cttcgcgtcg
     1261 aagatcgagt tcggtgcgga caacaagaag gcgaatgtga cccgcgaggt cgacggcggg
     1321 ctggagacga tcgaggtggc gctcccggcg gtggtgacca cggacctgcg gctgaacgag
     1381 ccgcgatacg cgtcgctccc cgggatcatg aaggcgaaga agaaggagtt gaaggtcgtc
     1441 ccggtggcat cgctgggcgt ggacacgacg ccgaaggtga agctgatctc gttcgcccca
     1501 ccgaagctgc gcgcgggagg cggccgggtg gcggatgccg cggaactggt gtcgaagctg
     1561 aaatccgagg cgaaggtcct ttaggaggat ttccatggcg gacatactgg tagtcgtcga
     1621 gcatcacgaa ggggtcttca agaagaacac gctctcggtc gtgtcggcgg ccaaggctct
     1681 ggcggggctg tccgggggcg aggtggacgc gctcgtcctt ggcgacggcg ccgcggcggc
     1741 ggtggccgac gtggcggccg gcacgggcgt gcggaaggtc ctgctgggcg agggagcggc
     1801 gttcgcgaaa tacctcgcgg cgacgtacgc gggcgcggtg gcgcaggtgg tgaaggcgaa
     1861 ggggtacggc gcggtgttcg caccggcctc cacgttcggg aaggatttca tgccg
//
LOCUS       DASVJL010000325         3350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1273088, whole genome shotgun sequence.
ACCESSION   DASVJL010000325 DASVJL010000000
VERSION     DASVJL010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273088"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VF357_06870"
     CDS             complement(<1..533)
                     /locus_tag="VF357_06870"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523906.1"
                     /translation="MKERIVRVFLRILEAIPLPVFAAFCETMMLLVCAVDRKHRRIAR
                     INLRIAFPEMGNAEADRIIRACYRRMGTSAAEFVHLPKMDAAYIREHFRIEGAEHVRE
                     SLEGRKQPAMAMTGHFGNWELLSHVYGATIAPAAFIVRPLKSDILDRIVTERRECVGN
                     TVIRKADSAKEVMKVLR"
     gene            complement(530..1522)
                     /gene="lpxK"
                     /locus_tag="VF357_06875"
     CDS             complement(530..1522)
                     /gene="lpxK"
                     /locus_tag="VF357_06875"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX7523907.1"
                     /translation="MILGAAARVRRILSSAGLLPASSLPRPVVSVGNLVMGGAGKTPH
                     VIHLARWLSGQGWRVGVLSRGYGRKSRGVRWVSDGEGPIATADEGGDEPVLIARSLPG
                     IPVAVGESRAAAGREVLSRRRVDVFLLDDGFQHLSLRRDVDLLLVECGRGLGNRWTAP
                     LGPLREPPSHARFADALVITKCPDAESGARTARSVPFPSVRPRAFSRLSPNGIVGRDG
                     LPSKEAAAGDAVFAFSGLARNAQFRDTLVTAGFRVKGFLPFPDHHAYARGDLDRIARE
                     AGGLPAITTEKDLVRLPGDVPFSVGALRVEVEYLDGWEEISRMILDRLEGGSRS"
     gene            complement(1519..2856)
                     /locus_tag="VF357_06880"
     CDS             complement(1519..2856)
                     /locus_tag="VF357_06880"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX7523908.1"
                     /translation="MDRPFLPGSPGHLLYNVLLGAGLAVGAPVWIPWVLLSRKRRANL
                     PDRIGLRGAPRQTPVDGRQTVWVHAVSVGETLAAVPLLRMLRRRVPDARLLVSSVTLT
                     GRETAVKSLSGVADEGFFFPFDFPGLCGRFLDRVRPDVVVIVETEIWPNFIAACARRG
                     IPVVIVNGRLSKRSFAGYIRFRWFFAPILRTLRTISAQTSEDAERFAALGAPREIITV
                     GGNLKFDVSPPESGASSLSALLLKEKGAGAIWIVAGSTHDGEEAQLLRAFLSAREGNP
                     SIRLLLAPRHPDRFDAVEALVRREGVTMVRRTAIPEGAGQLPETVLLLDTVGELSSAY
                     AAADLAFVGGSLVPKGGHNVLEPSWHGVPTIVGPHMENFREIADAFLAGDALIRVAGE
                     GELADRLTRFSANPFLFRETGLRAKQLLEMFRGASEATTDAVLSALPGRGAPG"
     gene            complement(2861..>3350)
                     /locus_tag="VF357_06885"
     CDS             complement(2861..>3350)
                     /locus_tag="VF357_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7523909.1"
                     /translation="VRNNIAYGMPGAPMEKVVEAARRANAHPFIERMPEGYGTVVGEQ
                     GLRLSGGERQRLAIARALLKDAPLLILDEATSALDTESEHVVQEALDALMHGRTTFVI
                     AHRLSTVRNADVIFVVEDGRIVERGGHEELLSRESRYRSFYLKQFEERKSDPPDGEAG
                     RG"
BASE COUNT          565 a         1195 c         1116 g          474 t
ORIGIN      
        1 ttccgcagga ccttcatcac ctccttcgcc gagtccgcct tgcggatgac cgtgttgccg
       61 acgcactccc tgcgttccgt gacgatccgg tcgaggatat cgctcttcaa cgggcgcacg
      121 atgaacgccg ccggggcgat cgtcgcgccg tagacgtgcg agagcagttc ccagttcccg
      181 aagtgcccgg tcatcgccat cgccggctgc ttccttccct ccagggactc ccggacgtgt
      241 tccgcgccct cgatccggaa gtgctcccgg atgtacgccg cgtccatctt cggtagatgg
      301 acgaactccg cggcggaggt gcccatccgc cggtagcacg cacggatgat ccgatccgcc
      361 tccgcgttcc ccatctccgg gaaggcgatc cgcaggttga tccgcgcgat ccgccggtgc
      421 ttccggtcga cggcgcagac aaggagcatc atcgtctcgc agaacgcggc gaacaccggg
      481 agcggtatcg cttcgaggat gcgcaggaag actcggacga ttctttcctt cacgaccggg
      541 agcctccctc caggcgatcg aggatcatcc gggagatctc ctcccacccg tcaaggtact
      601 ccacctccac gcgaagcgcg ccgacggaaa acgggacgtc gccggggaga cggaccaggt
      661 ccttttccgt cgtgatcgct ggcagaccgc cggcctcccg ggcgatccgg tcgagatccc
      721 cccgggcgta cgcgtgatgg tccggaaacg ggaggaatcc cttgacccgg aatcccgccg
      781 ttaccaaggt gtcgcggaac tgggcgtttc gtgccagccc ggagaacgcg aacacggcgt
      841 ccccggccgc ggcttccttt gaagggaggc cgtcccgccc cacgatcccg tttggcgaca
      901 ggcgcgaaaa ggccctgggg cggacggaag gaaaggggac ggaccgcgcg gtccgtgccc
      961 cggattccgc gtcgggacat ttcgtgatca ccagcgcgtc cgcgaaccgc gcgtgggacg
     1021 gcggctcccg cagcgggccc agcggcgccg tccaccggtt cccgagcccc ctgccgcact
     1081 cgaccaggag caggtcgacg tcgcgccgca gggaaaggtg ctggaatccg tcgtcgagga
     1141 ggaacacgtc cacgcgccgc ctggagagga cctcccgacc cgcggcggca cgggactcac
     1201 ccacggcgac cgggatccct ggcagggagc gggcgatcag gaccggctcg tccccaccct
     1261 cgtccgctgt ggcgatcggc ccttcaccgt cggagaccca ccggacgccg cggcttttcc
     1321 gtccgtatcc ccgggacagg actccgacgc gccacccctg ccccgagagc caccgcgcga
     1381 ggtggatgac atggggcgtc tttcccgcgc cgcccatcac gaggttcccg acgctcacga
     1441 ctggccgggg aagcgacgag gcgggaagga ggcccgccga ggaaaggatg cgccgcaccc
     1501 tggcggcggc cccgaggatc atcccggcgc cccgcggccc ggaagcgccg acaggaccgc
     1561 gtccgtggtc gcctcggagg cgccgcggaa catctcgagc agttgcttcg cgcgcaggcc
     1621 ggtctcgcgg aagaggaatg gatttgcgga gaatcgcgtc aaccgatccg cgagctcccc
     1681 ctcccccgcg acccggatca gggcatcccc ggccaggaat gcgtcggcga tctcccggaa
     1741 attctccatg tgcgggccga cgatcgtcgg cacaccgtgc cacgaaggct cgaggacgtt
     1801 gtgcccgccc ttcggaacga ggctgccccc gacgaacgcg aggtcggccg ccgcgtacgc
     1861 gctcgaaagc tcaccgaccg tgtcgagcag aagaaccgtt tccggaagct gccccgcccc
     1921 ctcggggatc gcagtgcgtc gcaccatggt aacgccctcc cggcggacca gggcttcgac
     1981 cgcatcgaac cgatcagggt ggcgcggcgc gaggaggagc cggatcgacg gattcccctc
     2041 ccgcgcggag aggaatgccc gcaggagctg cgcctcctcg ccgtcatgcg tcgaaccggc
     2101 gacgatccat atggcccccg cgcccttctc cttcaggaga agggcggaaa gcgacgacgc
     2161 cccggattcc gggggggaaa cgtcgaactt caggttcccc cccacggtga taatctcccg
     2221 cggggctccc agggcggcga accgctccgc atcttcggac gtctgggccg agatcgtccg
     2281 aagcgtccga aggatgggag cgaagaacca ccggaaccga atatacccgg cgaacgagcg
     2341 cttcgacaac cggccgttga cgatcaccac gggaatcccg cgtcgcgcgc acgcggcgat
     2401 gaaattcggc cagatctcgg tctcgacgat caccacgacg tccggccgga cccggtcgag
     2461 gaatcgcccg cagagccccg gaaagtcgaa cggaaagaag aatccttcgt cggcgacgcc
     2521 ggagagggat ttgaccgcgg tctcccgccc ggtgagcgtc acgctcgaga cgaggagccg
     2581 ggcgtcgggg acgcgccggc gcaacatccg aaggaggggg acggccgcga gagtctctcc
     2641 cacggaaacc gcgtgaaccc acacggtctg gcggccgtcg acgggcgttt gccggggggc
     2701 gccgcgcagt ccgatccggt ccggaaggtt cgccctccgc ttccgggaga ggaggaccca
     2761 cgggatccag acgggagcgc cgacggcaag tcccgccccg agcagaacgt tatagagaag
     2821 gtgccccggg ctcccgggga ggaacggtcg atccaccacc ctaccccctg cccgcttcgc
     2881 catccggggg atccgatttc cgctcctcga actgcttcag gtagaaggac cggtagcgtg
     2941 actcccgtga gagaagttcc tcgtgccccc cccgctcgac gatgcgcccg tcctcgacga
     3001 cgaagatcac gtccgcattg cggaccgtgg aaagccggtg ggcgatgacg aaggtcgtgc
     3061 ggccgtgcat gagggcgtcg agcgcctcct gcacgacgtg ctccgattcg gtgtcgagcg
     3121 cggaagtggc ctcgtcgagg atgagcagcg gggcgtcctt caggagcgcc cgggcgatcg
     3181 cgagccgctg gcgctccccc ccggacagcc gcagcccctg ttccccgaca accgtcccgt
     3241 atccttcggg catccgctcg atgaacggat gcgcgtttgc gcggcgtgcc gcctcgacga
     3301 ccttctccat cggggcgccg ggcatcccgt aggcgatgtt gttccggacc
//
LOCUS       DASVJL010000326         4960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_428879, whole genome shotgun sequence.
ACCESSION   DASVJL010000326 DASVJL010000000
VERSION     DASVJL010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428879"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /gene="accB"
                     /locus_tag="VF357_06890"
     CDS             complement(<1..414)
                     /gene="accB"
                     /locus_tag="VF357_06890"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX7523910.1"
                     /translation="MPMNLKEIREILELLKGSDVSEFELGRGDTVLKLRRGPANVPVA
                     LPPAAHASAVPVRPAEEPAAAPVPPKPTYKEILSPIVGTFYRSPAPDAAPFVEVGTRV
                     VKGQVLCIVEAMKIMNQIESDTTGTIAAIIVENAQP"
     gene            complement(419..976)
                     /gene="efp"
                     /locus_tag="VF357_06895"
     CDS             complement(419..976)
                     /gene="efp"
                     /locus_tag="VF357_06895"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX7523911.1"
                     /translation="MFTTSDFRNGLKIEFDGGPFLIVYFQHVKPGKGGAFVRTKLKNL
                     KTGAVIEHTFRSGDKVEKPDLEEREMQFMYKMEGQFHFMDTRTYEQIYLDEGHVEGAG
                     NYLIENLPVKILFYKGEPIGIDIPFFIDLKIVETEPGVRGDTVSGSTKPARLESGALV
                     SVPLFLNEGDVIKVDTRTGSYIERM"
     gene            complement(993..2066)
                     /locus_tag="VF357_06900"
     CDS             complement(993..2066)
                     /locus_tag="VF357_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX7523912.1"
                     /translation="MGGLDHRVERLLGVLRKRRLDAYLCVRLSNIRYLTRFTGSNAAL
                     VVSPAGPVLFTDGRYTEQARSEVRGAEVEVTPDPWNAAARRMRALRARRIGFESRHLS
                     VDSFRAVSRGRADRFVPIQDLVAAIRMRKEPGEILAIERATAIATASLLSELASGVRG
                     KTERDVAADLAREMIRRGAEGVSFPPIVADGPRSAMPHASPGGAAITGDGPLVLDFGA
                     RWNGYCSDETVTILPPRLRSPIGKAFDAVRRAQAAGISSVRPGEPCRMVDARVRDSLD
                     RSGYLKYFVHSTGHGVGLDVHERPSLSPRSRERLEEGMVVTVEPGVYLPGIGGIRLED
                     TVKVTGSGCERITFLPKTHTPLV"
     gene            complement(2053..2526)
                     /gene="aroQ"
                     /locus_tag="VF357_06905"
     CDS             complement(2053..2526)
                     /gene="aroQ"
                     /locus_tag="VF357_06905"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX7523913.1"
                     /translation="MKGKRAFRILFVDGPNLNVLGDREPSVYGRETPADIRKAVTATA
                     VAEGATVAFFQSNHEGEIVERLQAAKRRFDGVVINPAAYTHTSVAVRDALLYSGLPAI
                     EVHLTNPASREEFRKVSLVEDVVVGRICGVGGYGYTLALLALLRHLRREGARGRA"
     gene            complement(2523..2882)
                     /locus_tag="VF357_06910"
     CDS             complement(2523..2882)
                     /locus_tag="VF357_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523914.1"
                     /translation="MTFLEIFEEMHRKDQGVTSGALTGVDGLTVEEWRAPGDDRDLSA
                     LCAEIVQFFRESDRIAAENGLGAAEEVHLGGDRAQVFIRKVAAEYFLVIVTDPGTVPG
                     KCRFLLRQAARRTREML"
     gene            complement(2869..4452)
                     /gene="aroB"
                     /locus_tag="VF357_06915"
     CDS             complement(2869..4452)
                     /gene="aroB"
                     /locus_tag="VF357_06915"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX7523915.1"
                     /translation="MSSETMTVALGDRSYDIFFCREIYSLFQEWICRFYPGGTVHVVT
                     DRNVASIYGDDIRRWLEGIPHDVLALPPGEEHKNYDTVREIYGFLARGDAGRDSLVVA
                     FGGGVVGDLAGFAAATYLRGISCIQVPTTLLSQVDSSVGGKTGFNLPEGKNLVGAFHQ
                     PRAVFIDDVFLLTLDDRNLRAGMAEVVKCALAGDPELWDRLLAVGGKWKALSGEEWRW
                     VVRRAVAFKASVVERDEREASLRKVLNLGHTIGHAMETSGGYGRLLHGEAVAMGLAWE
                     AILGMKLGVTGGELVDDLVSLLIEMGFPLDDPGVPLTSIAAAIGMDKKRRVSDVDLPL
                     VAAPGRCELRRVPLAEIRRELPGIRAEIRERSIARELNVPAGNAIPEESPVSNLVHVG
                     TEVYEIRSVEENIAPAVPVVVGPAVGQEPAEHLAVPEPEQAPSEITAAPAVPVPEQAP
                     AETPAAPAVRTVTLADLYWSQGEHATARRIVGEILRDNPANLRAQAWLAARTGDDLVE
                     AELLGFLETMAKEYGYDVS"
     gene            complement(4449..>4960)
                     /locus_tag="VF357_06920"
     CDS             complement(4449..>4960)
                     /locus_tag="VF357_06920"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX7523916.1"
                     /translation="VLVGFMGSGKSSVGRELARRFGVPFVDVDEWIESAAGCRIRDLF
                     AREGEPAFREREKAALREVLSVKGCVIATGGGAFADEENRVLLRSYAPVVYLDTAVET
                     LIERLAADLGRPLLRGGDREEVVRALLSRRIPGYCTADVTVPTDGRTVEEVAGQVADW
                     IDRTEGRAG"
BASE COUNT          810 a         1791 c         1559 g          800 t
ORIGIN      
        1 cggctgcgcg ttttccacga tgatcgcggc gatcgtgccg gtggtgtccg actcgatctg
       61 gttcatgatc ttcatcgcct cgacgatgca cagcacctgt cccttgacca cgcgggtccc
      121 cacttcgacg aagggggcgg catcgggagc cggggaacgg tagaaggtcc ccacgatcgg
      181 ggagaggatc tccttgtagg tcggcttcgg gggaaccgga gccgccgccg gctcctccgc
      241 cgggcggacc gggactgccg aggcgtgcgc cgccggcggc agggcaacgg gaacgttcgc
      301 cggccccctg cgaagcttga gcaccgtgtc cccgcggccg agctcgaatt cgctcacgtc
      361 ggaacccttc agcaactcca ggatctcgcg gatctccttc aggttcatgg gcatcccctc
      421 acatccgctc gatgtagctt cctgtccgcg tgtccacctt gatcacatcc ccctcgttca
      481 gaaagagggg aaccgacacg agcgcgccgg actccagtct cgccggcttg gtcgaaccgc
      541 tcaccgtgtc cccgcggaca ccaggctcgg tttcgacgat cttcaggtcg atgaaaaaag
      601 ggatgtcgat cccgatcggc tcccccttgt agaagaggat cttcacgggg aggttctcga
      661 tcaggtagtt ccccgccccc tccacgtgtc cctcgtcaag gtaaatctgt tcgtacgtcc
      721 gcgtgtccat gaagtggaac tggccttcca tcttgtacat gaactgcatc tcgcgctctt
      781 cgaggtccgg cttctcgacc ttgtcgccgc tgcggaaggt gtgctcgatc accgccccgg
      841 tcttcaggtt cttgagcttc gttcggacga aggctccccc cttgccgggc ttcacgtgct
      901 ggaagtagac gatgaggaac gggcccccgt cgaactcgat cttcagcccg ttgcggaaat
      961 ccgacgtagt gaacatcctt gggcgcgcct cctcaaacca gtggtgtgtg cgtcttgggg
     1021 aggaacgtga ttcgttcgca ccccgacccg gtgaccttca ccgtgtcctc cagccggatc
     1081 cccccgatcc cgggaagata gacgccgggt tccaccgtga ctaccatccc ctcttcgagc
     1141 cgctcgcgcg accgggggga aagggaaggc cgctcatgca cgtcgagccc cacgccatgt
     1201 ccggtcgaat gaacaaaata cttcaaatat cccgaccggt ccagcgaatc ccgcacgcgc
     1261 gcatccacca tccggcacgg ttcgcccggc cggacggagg aaatcccggc cgcctgcgcc
     1321 cgacggacgg catcgaacgc cttcccgatg ggggagcgca ggcggggggg gaggatcgtc
     1381 accgtctcgt cggagcagta cccgttccag cgggcgccga aatcgaggac cagcgggccg
     1441 tccccggtga tcgccgcccc ccccgggctc gcgtggggca tcgcggagcg ggggccgtcc
     1501 gcgacgatcg gaggaaagga taccccctcc gctccccgac ggatcatctc ccgcgccagg
     1561 tccgcggcca cgtcacgctc cgttttcccc cgcactcccg aggcgagctc ggacagcagg
     1621 gatgcggtcg cgatcgccgt cgcgcgctcg atcgccagaa tctccccggg ttccttccgc
     1681 atccggatcg ccgcgacgag atcctggatg ggaacgaaac gatccgcccg cccacgggag
     1741 accgcccgga aggaatccac ggagagatga cgggactcga atccgatcct tcgcgcccgg
     1801 agggcgcgca tccgtcgggc ggcggcgttc catgggtcgg gagtcacttc cacttcggct
     1861 ccgcggacct ccgatcgggc ctgctcggtg tatctcccgt cggtgaacag gaccgggcct
     1921 gccggagaaa cgacgagtgc ggcgttgctc ccggtgaagc gcgtcaggta gcggatgttc
     1981 gagagccgga cgcagagata ggcgtccagg cggcgtttcc gcaggacccc caggaggcgc
     2041 tcgacgcggt gatcaagccc gcccacgagc cccttcccgc cgcaggtgac gcagcagcgc
     2101 gagcagggcg agggtgtagc cgtacccacc gacgccgcag atccggccga cgaccacgtc
     2161 ttcgaccagg gacactttcc ggaattcttc ccggctcgcg gggttggtga ggtgcacttc
     2221 gatggccggc aggccggagt agagcagggc gtcccggacg gccacgctcg tgtgggtgta
     2281 cgccgccgga ttgatcacga ccccgtcgaa gcgccgcttc gccgcctgga gccgctcgac
     2341 gatctccccc tcgtggttcg actggaagaa ggcgaccgtc gcgccctccg ccacggccgt
     2401 cgccgtcacc gctttgcgga tgtcggccgg cgtctcccgc ccgtagacgg acggctcgcg
     2461 atctccgaga acgttcaagt tgggaccgtc gacgaacagg atccggaacg ccctcttccc
     2521 tttcacagca tctcccgcgt gcgccgcgcc gcctggcgga gcaggaaccg gcacttcccg
     2581 gggaccgtcc ccggatccgt gacgatcacg agaaaatatt ccgcggccac cttgcggatg
     2641 aagacctgcg cgcggtcccc gccgagatgg acctcttccg ccgccccgag tccgttctcc
     2701 gccgcgatgc ggtcggattc ccggaagaac tggacgatct cggcgcaaag agcggagagg
     2761 tcccggtcgt cccccggcgc gcgccattcc tcgacggtca gcccgtccac ccctgtcagc
     2821 gcgccggagg tcacgccttg atccttccgg tgcatctcct cgaagatctc aagaaacgtc
     2881 atatccgtac tccttcgcca tggtctcgag aaacccgagg agttccgcct ccacgagatc
     2941 gtccccggtc cgggctgcca accacgcctg ggcccggagg tttgcggggt tgtcgcgaag
     3001 gatctccccg acgatccgcc tcgcggtcgc atgctctccc tgggaccagt acaggtcggc
     3061 cagggtcacc gtccgcactg cgggcgcggc gggggtttcc gccggcgcct gttccggtac
     3121 cggcacggcg ggcgcggcgg tgatctccga aggcgcctgt tccggttccg gcacggcgag
     3181 atgctccgcc ggttcctggc ccactgccgg cccgactacc acggggaccg cgggggcgat
     3241 gttctcctca acggagcgga tttcgtacac ctcggtcccg acatgaacaa ggttcgaaac
     3301 cggcgactct tccgggatcg catttcccgc cggaacgttg agctccctcg cgatcgagcg
     3361 ttcccggatc tccgcgcgga tcccgggcaa ctcccgccgg atctccgcga gggggacgcg
     3421 ccgcagttcg caacggcccg gggcggcgac cagcggcagg tccacgtccg acaccctccg
     3481 cttcttgtcc atcccgatgg cggcggcgat agaggtgagg ggcacccccg gatcgtcgag
     3541 ggggaacccc atctcgatga ggagcgagac aaggtcgtcg accagttccc cgccggtcac
     3601 gccgagcttc atcccgagga tcgcctccca ggccagcccc atcgccaccg cctcgccgtg
     3661 gaggaggcgg ccgtacccgc ccgatgtctc catcgcgtgg ccgatggtgt gcccgaggtt
     3721 gaggaccttg cgaagcgagg cctccctctc gtcccgctcg acgacggacg ccttgaacgc
     3781 gacggcgcgg cggacgaccc accgccactc ctccccggag agcgccttcc acttcccgcc
     3841 gaccgcgaga agccgatccc ataattcggg gtcgccggca agggcgcatt tgaccacctc
     3901 ggccatccct gcgcggagat tgcgatcgtc gagggtcagc aggaagacgt cgtcgatgaa
     3961 gaccgcccgg ggctggtgga acgccccgac gaggttcttc ccctcgggga ggttgaagcc
     4021 ggtctttccg cccacgctgc tgtccacctg ggaaaggagg gtcgtaggca cctggataca
     4081 ggagatcccc cgcaggtacg tcgcggcggc gaatccggcg aggtcgccga cgaccccgcc
     4141 gccgaaggca acgacgaggg aatcccgccc ggcgtccccg cgcgccagga atccgtagat
     4201 ctccctcacc gtgtcgtagt tcttgtgctc ctcccccggg gggagggcca ggacgtcatg
     4261 ggggatcccc tcgagccacc gccggatgtc gtccccgtag atggaggcga cattgcgatc
     4321 ggtcaccacg tggaccgtgc cgccgggata aaaacggcag atccattcct ggaacaggga
     4381 gtagatctcc ctgcagaaga agatgtcgta ggaacggtcg ccaagggcca ccgtcatcgt
     4441 ttcagagctc atcccgcccg tccctccgtc cggtcgatcc aatccgccac ctgcccggcc
     4501 acttcctcca ccgttcgccc gtcggtcggc accgtaacgt cggcggtgca atacccgggg
     4561 atccggcgcg acagcagcgc ccgcaccacc tcttcccgat cccccccgcg aagcagcgga
     4621 cgcccgagat ccgcggcaag ccgctcgatg agggtctcca cagccgtgtc gaggtagacc
     4681 accggggcgt aggaacggag gagaacccgg ttctcctcgt cggcgaacgc cccgcccccc
     4741 gtcgcgatca cgcacccttt caccgagaga acttcgcgca gggcggcttt ctcccgctcg
     4801 cgaaacgccg gctccccctc ccgggcgaac agatcccgga tcctgcaccc ggccgccgac
     4861 tcgatccatt cgtccacgtc gacgaacggc acgccgaacc gtcgcgccag ctcccggccg
     4921 acggagcttt tccccgaccc catgaacccg accagcacga
//
LOCUS       DASVJL010000327         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_709978, whole genome shotgun sequence.
ACCESSION   DASVJL010000327 DASVJL010000000
VERSION     DASVJL010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709978"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VF357_06925"
     CDS             <1..184
                     /locus_tag="VF357_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523917.1"
                     /translation="LAAAMLASTLGVEFDEDKSWDEKREVWKISGKIYRSFNITQSAI
                     VKDEYTTVVAAAVLVS"
     gene            199..1191
                     /locus_tag="VF357_06930"
     CDS             199..1191
                     /locus_tag="VF357_06930"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX7523918.1"
                     /translation="MRGARIIGTGRALPPRVVKNEELTQRMDTSDEWIVQRTGVRERR
                     YVDPGTGSSHLGAEAARKAIANAGITVDDIDLIVFATLSPDHVFPGNGVLVQQQLGMR
                     TVGALDVRDQCTGFVYGLSVAEAYVKGGFHDHVLVIGAEVHSTGLDFSTKGRDVAVIF
                     GDGAGAVVVGPAEAGRGILSSHLHSEGKYAKELWLEGPSFLDNPWISHEMIDAGKHFP
                     KMNGRYVFTHAVRRFPEVIREALEKNGLTVSDLSLLIPHQANLRITQAVGVALLLPEE
                     KVFSNVEQYGNTTAASIPIALDECVEQGRIREGDIVCLASFGSGFTWASTLLRW"
     gene            1216..1659
                     /gene="rimI"
                     /locus_tag="VF357_06935"
     CDS             1216..1659
                     /gene="rimI"
                     /locus_tag="VF357_06935"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX7523919.1"
                     /translation="MGTSDLDGVMAIEEVSFPTPWSRGMFIEDFPRDFSDALVAAGTE
                     DEVLGYAVCWTIAGESHLLNIAVHPSRRGEGIGRALLSECIRRAARAEASLIFLEVRA
                     GNEAAQRLYRSMGFAFQGIRKGYYTDTGEDAVILEREVRKSDAAR"
     gene            1646..2464
                     /locus_tag="VF357_06940"
     CDS             1646..2464
                     /locus_tag="VF357_06940"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523920.1"
                     /translation="MPHGNPRFVHGKILHNTGSGIFYRMEVAIPERIDFVPGQFAMVS
                     GWPGNDPLLPRPLAIFRSGGVRGKGTVECVYKVVGRGTALLSGLHTGDPLALTLPLGH
                     GFEFPGGDRAWWLVGGGVGFSSVFPAAAVLDAQRADFEIFLGARTRDQLPPVEWIPGG
                     AVPGRVHLCTDDGTAGFFGTAAAALRDRIDRRKSSGPAGVAILACGPREMLKAVAELA
                     LPLDIGVQVSLESSMACGFGVCWGCVTAVRDGEISGYRRVCREGPVFDAREVVW"
     gene            2461..3393
                     /locus_tag="VF357_06945"
     CDS             2461..3393
                     /locus_tag="VF357_06945"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX7523921.1"
                     /translation="MTQSLTPDLSARVGSVLLKNPVMSASGTFGYGLEFMPFYDISRL
                     GAVIVKGLSLLPTPGNAPGRIVETPAGMLNAIGLQNIGVERFVNEVAPRLDDAGAVYV
                     ANIYGRTIEEYEAVARRLSDLPALAAIELNASCPNVKEGGIAFGSNPEGLSGLTRRVR
                     AATGKPLWVKLSPNVSDIGVIARAAEEAGADAVTLINTIVGMAVDPRTRRPMLSNVTG
                     GLSGPAVKPVALRMVWEACKAVKIPVVGIGGIQSAGDALEFLLVGAAAVQVGTANFRN
                     PNACVEIVDGIRDFFVREKIFRLDDYRGTLLLRE"
     gene            3497..3979
                     /gene="rimP"
                     /locus_tag="VF357_06950"
     CDS             3497..3979
                     /gene="rimP"
                     /locus_tag="VF357_06950"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX7523922.1"
                     /translation="MDTLKIEALAGEVARDLSLSLFDVEIAREGPRTILRVFVEREGG
                     VPLSDIVTFSRRFSAILEVDDPMDGPYVLEVSSPGVTRRLRRPEHFERSLGKRVRVNL
                     SAPREGRRHLVGELSGCDGEGIEVVSDGTTYRVPYGEIRKATLDVTQEELFGKGKKTR
                     "
     gene            3976..>4176
                     /locus_tag="VF357_06955"
     CDS             3976..>4176
                     /locus_tag="VF357_06955"
                     /inference="COORDINATES: protein motif:HMM:NF020118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEX7523923.1"
                     /translation="MILDVGQIIAQLGKEKGIDKTIIVAAIKEALETAARKKYGMNKI
                     LEATYDSDTGEFEILSFRTATET"
BASE COUNT          676 a         1279 c         1504 g          717 t
ORIGIN      
        1 cctggcggcg gcgatgctcg cgtcgacgct cggcgtcgag ttcgacgagg acaagagctg
       61 ggacgagaag cgggaggtgt ggaagatcag cgggaagatc taccgctcct tcaacatcac
      121 gcagtcggcc atcgtcaagg atgagtacac cacggtcgtg gccgcggccg tgctggtctc
      181 gtgatggaag gagctggaat gcgcggagcg cggatcatcg gcacggggcg ggctcttcct
      241 cctcgtgtgg tgaagaacga ggaactgacg cagcggatgg atacgtcgga cgagtggatc
      301 gtccagcgca ccggggtccg ggagcgcagg tacgtcgatc ccgggacggg ctcgtcccac
      361 ctcggcgccg aagccgcgcg caaggcgatc gcgaacgccg ggatcacggt ggacgacatc
      421 gacctgatcg tcttcgccac cctctcgccg gatcacgtct tccccgggaa cggtgtcctg
      481 gtccagcagc agctcgggat gcggaccgtg ggcgccctcg acgtgcgcga ccagtgcaca
      541 ggctttgtct acggtctctc cgtggcggag gcgtacgtca agggcgggtt ccatgaccat
      601 gtcctggtga tcggggccga ggtgcatagc accgggctcg atttcagcac gaaggggcgc
      661 gacgtggccg tgatcttcgg ggacggcgcg ggggcggtcg tcgtcggccc cgcggaggcg
      721 gggaggggga tcctctcttc ccaccttcat tccgagggga aatacgccaa ggaactgtgg
      781 ctcgaagggc ccagttttct cgataacccc tggatttccc acgagatgat cgacgccggg
      841 aaacatttcc cgaagatgaa cggccggtac gtcttcaccc atgcggtcag gcgcttcccc
      901 gaggtgatcc gcgaagccct cgaaaagaat ggcctcaccg tatcggacct ctccctgctc
      961 attccccacc aggcgaacct gcgcatcacc caggcggtcg gggtcgcgct cctgctgccc
     1021 gaggaaaagg tgttctccaa cgtcgagcag tacgggaaca cgacggcggc gtcgatcccg
     1081 atcgcgctcg acgagtgcgt cgagcagggc cggatcaggg agggggacat cgtctgcctc
     1141 gcctcgttcg gttccgggtt cacctgggcc tcgacgcttc tccgctggta gagtggggac
     1201 atgcggatcc gggagatggg cacgtcggac ctcgacggcg tgatggcgat cgaggaggta
     1261 tccttcccga cgccctggtc ccgggggatg ttcatcgagg atttcccgcg cgacttctcc
     1321 gacgccctgg tcgcggcggg gacggaggac gaggtcctcg gatacgccgt ctgctggacc
     1381 atcgcggggg agtcccacct gttgaacatc gccgtgcacc cctcgaggcg gggggaggga
     1441 atcggccggg ccctcctgtc cgagtgcatc cgacgcgcgg cgcgggcgga ggcctcgctg
     1501 atcttcctgg aggtgcgcgc cggaaacgag gccgcccagc ggctttaccg ttcgatgggg
     1561 ttcgcgttcc aggggatccg gaaagggtat tacaccgaca ccggagagga tgccgtcatc
     1621 ctcgagcggg aggtacggaa aagcgatgcc gcacggtaac cctcggttcg tccacggaaa
     1681 gatccttcac aacacgggga gcggcatctt ctaccggatg gaggtggcga tcccggaacg
     1741 gatcgacttc gtccccggtc agttcgcgat ggtctcgggg tggccgggga acgatccgct
     1801 gctgccgcgg cctcttgcga tcttccgctc cggcggcgtt cggggaaaag ggaccgtcga
     1861 gtgcgtgtac aaggtcgtcg ggcgcggtac cgccctcctg tccggcctcc acaccggcga
     1921 tcctctcgct ctcacgcttc cgctcggaca cggtttcgag ttccccggcg gggaccgtgc
     1981 ctggtggctg gtggggggcg gcgtcggctt ctccagcgtc ttcccggccg cggcggtgct
     2041 cgatgcgcaa cgggccgact tcgagatctt cctcggggcg cggacccggg accagcttcc
     2101 cccggtggag tggatccccg gcggcgccgt tccgggccgg gtgcacctgt gcaccgacga
     2161 cgggacggcg ggattcttcg ggaccgcggc ggcggccctc cgggaccgga tcgaccggcg
     2221 gaaatcgtcc gggccggcgg gcgtcgcaat cctcgcgtgc gggccccgcg agatgctgaa
     2281 ggccgtcgcg gaactcgccc tccccctcga cattggtgtt caggtgtccc tcgaaagctc
     2341 gatggcgtgc ggcttcggcg tctgctgggg gtgcgtcacg gcggttcggg acggtgagat
     2401 atccggatac cgcagggtct gcagggaagg accggtcttc gacgcgaggg aggtcgtctg
     2461 gtgacgcagt ccttgacccc cgacctctcc gcgcgcgtgg ggagcgtgct cctcaagaac
     2521 ccggtgatga gcgcctccgg gaccttcgga tacggtctgg aattcatgcc gttctacgac
     2581 atctcgcgac tgggggcggt gatcgtcaag ggcttgtccc tcctcccgac accgggcaac
     2641 gcccctgggc ggatcgtgga gacccccgcg gggatgctga acgcgatcgg cctgcagaac
     2701 atcggggtcg agcggttcgt gaacgaggtg gcgccgcggc tggacgatgc cggcgccgtg
     2761 tacgtcgcga acatctacgg acgtacgatc gaggagtacg aggcggtggc gcgtcggctc
     2821 tccgatctcc ccgcgctggc ggcgatcgag ctgaacgcct cctgccccaa cgtgaaggag
     2881 ggggggatcg ccttcggatc gaaccccgaa gggctttccg gactcacccg ccgggtgcgt
     2941 gccgccacgg ggaaaccgct gtgggtgaag ctctccccga acgtgtcgga catcggtgtg
     3001 atcgcgcgcg cggccgaaga ggcgggggcc gatgcggtga ctctcatcaa caccatcgtc
     3061 gggatggcgg tggacccccg gacgcgccgg ccgatgctct ccaacgtgac cggaggcctc
     3121 tccggtccgg cggtgaagcc ggtggcgctt cggatggtat gggaggcgtg caaggcggtg
     3181 aagatcccgg tggtcgggat cggggggatc cagagcgccg gggacgccct cgagttcctg
     3241 ctggtgggcg ccgccgcggt gcaggtgggg acagcgaact tccgcaaccc gaacgcgtgc
     3301 gtcgagatcg tcgacgggat ccgggatttt ttcgtccggg agaagatctt ccgcctcgac
     3361 gactaccgcg ggacgctcct cctgcgggag taaaagaaat gtgttgtggg aaacgccggt
     3421 ttccctgtag tatgatagag tttggggctt ggaacggtgg gctggagccc actttttttt
     3481 tccttctcct gtgactatgg acaccttgaa gatcgaggcg ttggccgggg aagtcgcccg
     3541 ggatctgtcc ctttccctgt tcgacgtcga aatcgcaagg gaagggccga ggactatcct
     3601 ccgggtattc gtggagcgtg agggcggggt ccctctttcc gacatcgtga cgttcagccg
     3661 ccgtttcagc gcgatcctcg aggtcgacga tcccatggac ggcccctacg tgctcgaggt
     3721 ttcttccccc ggggtgaccc ggcggctgcg gcggccggag cacttcgaac ggtccctggg
     3781 gaagcgggtc cgggtgaacc tctcggcgcc gcgggagggc cggcggcacc tcgtcgggga
     3841 gctctccggg tgcgacggcg aggggatcga ggttgtgtcg gacgggacga cgtaccgggt
     3901 gccgtacggg gagatccgga aggcgaccct ggatgtgacg caggaagaac tcttcgggaa
     3961 gggaaagaaa acgcgatgat cctggacgtg ggacagatca ttgcccagct gggcaaggaa
     4021 aaagggatcg acaagaccat catcgttgcg gcgatcaagg aagccctcga gaccgccgcc
     4081 cggaagaagt acgggatgaa caagatcctc gaggcgacct acgattccga caccggggag
     4141 ttcgagatcc tgtcgttccg cacggcgacg gagacg
//
LOCUS       DASVJL010000328         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1366827, whole genome shotgun sequence.
ACCESSION   DASVJL010000328 DASVJL010000000
VERSION     DASVJL010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366827"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1280
                     /locus_tag="VF357_06960"
     CDS             105..1280
                     /locus_tag="VF357_06960"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7523924.1"
                     /translation="MSPDRFLKTIAAALLSSLLLPGPRAWGDEFRVTPSLGAKNEYNS
                     NVFFTTGARTDSFILTVSPRLEVSDRTERLDALLDAGADRLFYSTDRELDTWDQRYRG
                     ALGYRISDGLRLSGDASWRREGRPDRDIGTTGLLVGERSDRLIGSATIDFALGEKTGG
                     GLSYGYDRIDYQSQSNVNSEQHTVGLGFTHDLGRYLSNTKARANLGYSRGRFTGLDVE
                     NYTVTLGFSREVHELWSVLADVGARYTRSQFDVPGAESSDDTGWIAKVALAYKWERGT
                     GNLSFYRDVTVAAGQGGAVERTAVTLDLAHRGSYEFTGLFSAGYYVNRSEAGQFSGQA
                     IDERTVLVRPGLRYAATKNIDVEAAYQYTRVLYRQSDTAASQNVVYLLAALRYPVVE"
     gene            1303..>1654
                     /locus_tag="VF357_06965"
     CDS             1303..>1654
                     /locus_tag="VF357_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX7523925.1"
                     /translation="MNQDQATDQDVKTFQDLADVFRRRKREIFLPAFGIFALAAVVAF
                     ALPRTYKSTTTVLIEEQEVPREYVSANIASFADQRLQSINQRIMSSTKLLDLISRFQL
                     YPDLKDKVPADAIVA"
BASE COUNT          297 a          549 c          553 g          255 t
ORIGIN      
        1 gaaagggggg gtgaccacca tgtctcccct gttcaactac gacgatgtga cggcggggca
       61 cggcatcgag acgaacgtcc agctggagcg cggggacctg atctttgtcc cctgaccggt
      121 tcctgaagac gatcgcggcg gcgctcctct cgtcgctcct ccttcccggc ccgcgggcgt
      181 ggggggacga gttccgggtc acgccgtcgt tgggggcgaa gaacgagtac aacagcaacg
      241 tcttcttcac caccggcgcc cggaccgatt cgttcatcct caccgtctcc ccgcgcctgg
      301 aggtatcgga ccgcaccgag cgtctggacg cgctcctcga cgcgggcgcg gaccggctgt
      361 tctacagcac cgaccgggaa ctggacacgt gggaccagag gtaccgtggg gcgctgggct
      421 acaggatctc cgacgggctc cggctatccg gcgacgcgtc ctggcgcagg gaaggccgcc
      481 ccgaccgcga catcgggacg accggcctcc tcgtcggcga gcgcagcgac cggctgatcg
      541 gttcggcgac gatcgatttc gccctgggcg agaagaccgg gggggggctc tcctacgggt
      601 acgaccggat cgactaccag agccagtcga acgtgaacag cgagcagcac acggtggggc
      661 tcggcttcac ccacgacctg ggccggtacc tttccaacac gaaggcccgg gcgaacctcg
      721 gatactcccg gggccggttc accggactcg atgtcgagaa ttacaccgtg accctgggat
      781 tcagccggga ggtccacgaa ttgtggagcg tgctcgccga cgtcggcgcc cgctacaccc
      841 ggtcgcagtt tgacgtgccg ggcgcggaat cgagcgacga cacggggtgg atcgccaagg
      901 tcgcgctggc gtacaagtgg gaacgcggca ccggcaacct gtcgttctac cgcgatgtta
      961 ccgtcgcggc ggggcagggc ggggcggtcg agcggaccgc cgtcacgctg gacctggccc
     1021 accgcgggag ctacgagttc accggcctct tctcggccgg ctactacgtg aaccggtcgg
     1081 aggcggggca attctccggg caggcgatcg acgagcggac ggtcctcgtg cggcccggcc
     1141 tgcggtacgc cgccacgaaa aacatcgacg tggaagcggc gtaccagtac acgcgggtcc
     1201 tgtatcggca gtcggatacg gcggcaagcc agaacgtcgt gtacctgctc gcggccttgc
     1261 ggtacccggt ggtcgaatga gaagagatcc caaggagtgc cgatgaacca ggaccaggca
     1321 acggatcaag acgtaaagac ctttcaagac ctcgccgacg ttttccgcag gcggaagcgg
     1381 gagatcttcc tgcccgcgtt cggcatcttc gcgctggcgg cggtcgtggc cttcgccctc
     1441 cccaggacgt acaaatccac cacgacggtc ctgatcgagg agcaggaagt cccgcgcgaa
     1501 tacgtctcgg ccaacatcgc ctccttcgcc gaccagcgcc tgcagtcgat caaccagcgg
     1561 atcatgagca gcacgaagct gctcgatctc atctcccgct tccagctgta cccggacctg
     1621 aaagacaagg tgccggccga cgcgatcgtc gcga
//
LOCUS       DASVJL010000329         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1648076, whole genome shotgun sequence.
ACCESSION   DASVJL010000329 DASVJL010000000
VERSION     DASVJL010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648076"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VF357_06970"
     CDS             complement(<1..248)
                     /locus_tag="VF357_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX7523926.1"
                     /translation="MYSVGDSAEFTKVFSEEDVFLFAGITGDRNPVHTSREFAAKTRF
                     KERIAHGLLTAGTISAAIGMKLPGPGCLYLSQTLEFKAP"
     gene            complement(525..>2205)
                     /locus_tag="VF357_06975"
     CDS             complement(525..>2205)
                     /locus_tag="VF357_06975"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7523927.1"
                     /translation="AAERLLGMEKGKALGKNLLSIVPNSGLVNVLRTGEAETGRPQVV
                     GARTVIANRAPMFRDGELIGAVSVFQDVTEMEKMSRELDSTRALVRTLEEVLGGAGEW
                     MVVVDANGIVTMISEEYAEFNGTTVADAVGKHATQVIENTRMHVVAKTGVAEIGERQT
                     IHGRSLIVNRIPLKDGERVIGAYGRVVFKTVEQLRELASKMNLLESKVKYYEEELTHL
                     RGARYTFASIVGVGAAITAAKEEAERASRTDSTVLLRGETGTGKELFAHAIHAAGPRR
                     AGPFIKLNCAAVPAELLESELFGYEEGAFTGARRGGKPGKFELAAGGTLFLDEIGDMP
                     LPMQAKLLRVLQEKEVDRLGGAGSRRVDLRLIAATGRNLEEMVGQGTFRADLYYRVNV
                     IPVRIPPLREHPEDLGAIAEAFLARLSADTGEPKRRLSPELLDILRAYPWPGNVRELQ
                     NGLERAVAMSPREVLRPEHFPAHQLRFAHGARQEATPGATEADGEETGSKAPGSLASV
                     KAEAERSAILSALAAVGGNRTRAAELLRIHRVKLHEKIKRYGIAGPPGAKK"
BASE COUNT          368 a          767 c          663 g          407 t
ORIGIN      
        1 ggcgccttga actcgagggt ctgcgacaga tagaggcacc ccggccccgg gagcttcatc
       61 ccgatcgccg ccgagatcgt ccccgccgtg aggagaccgt gcgcgatccg ctccttgaag
      121 cgggtcttcg ccgcgaactc ccttgaagtg tgaacgggat tccggtcccc cgtgatcccg
      181 gcgaacagga agacgtcttc ctcggagaag actttcgtga attctgcgga gtctcccacg
      241 ctgtacattc gctgctcctc aatgctagat ggaacaagac ttcaacaaac attgttttgc
      301 aatagcggtg ccaagaagaa tcaggcgaat cctctccgga ttttaaaatc taaccaattg
      361 aataacaaca tgattatcaa atatggccgg gaaatcccat gtagagcaga agtgtaccga
      421 tagaggtact aattgctcca ggaaaagcag gcgtcccgga tttccgttgt cgtgaaggcc
      481 acctatacgg gagtgtacct ttattgttat atgtaccaga aaagttactt ctttgccccc
      541 ggaggacccg cgattccgta ccgctttatc ttctcgtgca acttcacgcg atggatccga
      601 agaagctccg ccgccctcgt cctgttcccc ccgaccgccg cgagggccga caggatggcg
      661 gaacgctccg cttcggcctt cacggaagcg agcgatccgg gggccttcga ccctgtttct
      721 tccccgtcgg cttcggtcgc ccccggagtt gcctcctgcc gcgcgccgtg cgcgaaccgc
      781 agctggtggg cgggaaaatg ctccggccgc aagacctcgc ggggcgacat cgccaccgcc
      841 cgctcgagtc cgttctgcag ctcccggacg ttcccgggcc agggatacgc tcgaagaata
      901 tcgaggagct cgggggaaag acgacgtttc ggctcgccgg tgtcggcgga gagccgggcg
      961 aggaacgcct cggcgatcgc gcccaggtcc tccggatgct cccgcagcgg cggaatgcgg
     1021 accgggatta cgttgacgcg gtaatagagg tcggcgcgga acgtcccctg ccctaccatc
     1081 tcttcgaggt tccggccggt ggccgcgatc aggcggaggt ccacccggcg ggaaccggcc
     1141 ccccccaggc ggtctacctc cttttcctgc aggacccgca gaagcttcgc ctgcatcggc
     1201 agaggcatgt cgccgatctc gtcgaggaag agggtcccgc ccgcggcgag ttcgaacttg
     1261 ccgggcttcc cgcccctgcg cgcgccggtg aacgctccct cctcgtagcc gaacagctcc
     1321 gactcgagca gctccgcggg gacggcggcg cagttgagct tgatgaacgg ccccgcgcgc
     1381 cgggggcccg cggcgtggat ggcgtgggcg aacagctcct tccccgtccc ggtttccccc
     1441 cgcagcagaa ccgtcgaatc ggtccgggag gcccgctccg cctcctcctt ggccgccgtg
     1501 atcgcggcgc ccacgccgac gatgctggcg aaggtgtacc gggcgccccg caggtgcgtg
     1561 agctcctcct cgtaatattt caccttcgac tcgaggaggt tcatcttcga cgccagctcc
     1621 cggagctgct ccacggtctt gaagaccacc cggccgtacg ccccgatgac ccgctcgccg
     1681 tccttcaggg ggatccggtt gacgatcagg gagcggccgt ggatcgtctg ccgctcgccg
     1741 atctccgcca caccggtctt cgcgacgacg tgcatccggg tgttctcgat cacctgcgtc
     1801 gcatgcttcc ccacggcgtc ggcgaccgtg gtcccgttga actccgcgta ctcttcgctg
     1861 atcatcgtga cgatcccgtt ggcgtccacg accaccatcc actcccccgc gccgccgagc
     1921 acctcctcga gcgtccggac cagcgcgcgc gtggaatcga gctccctcga catcttctcc
     1981 atttcggtca cgtcctggaa gacggacacg gcgccgatca gttccccgtc ccggaacatc
     2041 ggggcccggt tcgcgatgac ggtccgggcg ccgaccacct ggggtcgtcc cgtctccgcc
     2101 tcccccgtcc gcaggacgtt caccagcccc gaattcggaa cgatggagag aaggttcttc
     2161 ccgagggcct ttcccttctc catgccgagg aggcgctcgg cggcg
//
LOCUS       DASVJL010000330         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_710664, whole genome shotgun sequence.
ACCESSION   DASVJL010000330 DASVJL010000000
VERSION     DASVJL010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710664"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2104)
                     /locus_tag="VF357_06980"
     CDS             complement(<1..2104)
                     /locus_tag="VF357_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX7523928.1"
                     /translation="MTIRDGNILFFDGACGTNLQRMEIPMSAWEGRDGCNEFLNVSAP
                     EVIQEWHSSFLSAGATVLETNTFGANAIVLAEYGLADRVAEINRAAVGNAREAIRRHG
                     GPAYVAGSIGPTTKLPSLGHVAFEPMAAAVSEQARALVEAGADLLIFETCQDLLQIKI
                     ALVSCFGTLEQMKRDVPVMVSITIESTGTMLVGTDVAAALAAIEPYPVFSIGLNCATG
                     PEGMTSHLRYLCRHYRGRVSCIPNAGIPQVRDGKTHYPLSPEAFASQLSAFVRDDGVS
                     IVGGCCGTTPEHIRKLREALDGVAPVPRNVVEKPSLSSLYQAAEIRQEIPPFLVGERC
                     NANGSRRFRDLLLADDYPGALRVALEQEKDGAHAVDLCTAYAGRDEKADLSAMTRLFA
                     QSVQVPMVIDSTTPDCIESALKIHPGRCLVNSVNLEDGGKNLERVCRLAKTYGAAVIA
                     LTIHEKGMALTVEEKVATARAIHDLAVNRYGLRPSDLLFDVLTFTIGSGDATLVDAAA
                     NTLAAIRRVKEELPGVFTLLGVSNISFGLSPASRRVLNSVFLHEAVAAGLDAAIIDAA
                     KVLPMSKIPDADREICLDLIHNRRGTGAESPLSAFLRHFSDAKPVKEDSAEDRDLPAI
                     PPEEELAEKVVAGDKEGLEDLLSILLSRRPPASIINQLLVPAMRQVGELFGRGEMLLP
                     FVLQSAEVMKRSVDYLAPF"
     gene            2349..>2546
                     /locus_tag="VF357_06985"
     CDS             2349..>2546
                     /locus_tag="VF357_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523929.1"
                     /translation="MSNLKAKLLEKIQAHRPRTTKLVKEQGKIVIDQVTIDQCIGGAR
                     DIRSLVTDISYLDPQEGIRFRG"
BASE COUNT          451 a          834 c          837 g          424 t
ORIGIN      
        1 tgaagggggc gaggtagtcg acggaccgct tcatcacctc ggcggactgg aggacgaagg
       61 ggaggagcat ctcgcccctc ccgaagagct ccccgacctg gcgcatcgcc gggaccagca
      121 gctggttgat gatcgacgcc ggcggccggc gcgacaggag gatggagaga agatcctcca
      181 gtccctcctt gtctcccgcc accacctttt ccgccagttc ctcttccggg gggatcgcgg
      241 ggaggtccct gtcctcggcg gaatcctcct tcacgggctt tgcgtccgag aaatgacgga
      301 ggaaggcgga gaggggggac tccgcgccgg taccccggcg gttgtggatc aggtccaggc
      361 agatttcccg gtcggcgtcg gggatcttcg acatcggcag gaccttcgcc gcgtcgatga
      421 tcgccgcgtc gagtccggcg gcgaccgctt cgtgcaggaa gacggagttc agcacccggc
      481 gggaggcggg ggagaggccg aaggagatgt tgctcacgcc gagcagggtg aacacgccgg
      541 ggagttcctc cttcacccgc cggatcgccg cgagggtgtt cgcggcggcg tcgacgaggg
      601 tcgcatcccc cgatccgatg gtgaaggtga gaacgtcgaa gaggaggtcc gaagggcgca
      661 gcccgtaccg gttcacggcg aggtcgtgga tcgccctcgc ggtcgcgacc ttctcctcga
      721 ccgtcaacgc catccccttc tcgtggatgg tgagggcgat gaccgccgcc ccgtacgttt
      781 tcgccagccg gcagacgcgc tcgaggttct tccctccgtc ctcgaggttc accgagttga
      841 cgaggcacct cccggggtgg atcttcaacg ccgactcgat gcagtcgggg gtggtcgagt
      901 cgatcaccat cgggacctga accgactgcg cgaagagccg cgtcatggcg gagaggtccg
      961 ccttctcgtc cctccccgcg tacgccgtgc acaggtccac ggcgtgggcg ccgtctttct
     1021 cctgctcgag ggcgacccgc agcgcccccg ggtagtcgtc cgcgaggagc aggtcgcgga
     1081 accgcctcga gccgttcgcg ttgcaccgct ccccgacgag gaacggggga atctcctggc
     1141 ggatctccgc ggcctggtag aggctggaaa gcgacggttt ctccaccacg ttccgcggga
     1201 ccggggcgac tccgtcgagc gcctcgcgga gtttccggat gtgctccggc gtggtgccgc
     1261 agcacccacc gacgatcgat accccgtcgt ctcgaacgaa ggcggaaagc tgcgaggcga
     1321 acgcctcggg ggaaagaggg taatgggtct tcccgtcccg cacctgcggg atcccggcgt
     1381 tggggatgca ggagacgcgg ccgcgataat ggcggcaaag gtaccggagg tgggaggtca
     1441 tcccctcggg gccggtggcg cagttcagcc cgatcgagaa tacggggtac ggctcgatcg
     1501 cggccagggc ggcggcgacg tcggtcccca ccagcatcgt cccggtgctc tcgatcgtaa
     1561 tggagaccat cacggggacg tcccgcttca tctgctcgag cgtcccgaaa caggagacca
     1621 gcgcgatctt gatctgcagc aggtcctggc acgtctcgaa gatcaacagg tccgccccgg
     1681 cttcgacgag tgcgcgcgcc tgctccgaga cggcggccgc catcggctcg aaggcgacgt
     1741 gtcccagcga cgggagcttc gtggtgggcc cgatggagcc ggccacgtac gcgggccccc
     1801 cgtgtcgacg gatcgcctcc cgcgcgtttc cgaccgccgc ccggttgatc tccgcgaccc
     1861 ggtcggccag tccgtactcc gcgagaacga tcgcgttggc gccgaaggtg ttcgtctcga
     1921 gaaccgtcgc ccccgcggag aggaaagagg aatgccactc ctggatcacc tcgggtgctg
     1981 aaacgttgag gaattcgttg cacccgtccc gcccctccca ggcggacatg gggatctcca
     2041 tccgctgcag gttcgtcccg cacgccccgt cgaaaaacag gatgttcccg tcccggattg
     2101 tcatccccct cccctcggta agtgctcaat cttagaacag aaatcagtcg cagtcaaacg
     2161 ggttttcttt cctcttgact tgtcggatcc tttcctgcta agtagatagc atccgtgtat
     2221 accgtataca actcacatcc ctgatttcac ggcccataat tagacacagt cgatagcgat
     2281 accacccgaa aggaggcagg accatggcac cggtcgtaga aaaagccgac gaaaggagaa
     2341 gcgcaaacgt gtcaaacctc aaggcgaaac tcttggagaa gattcaggcc caccggccgc
     2401 gcacgaccaa actggtcaag gagcagggga agatcgtgat cgaccaggtg accatcgacc
     2461 agtgcatcgg cggcgcccgc gacatccgct ccctcgtgac cgacatttcc tacctcgacc
     2521 cgcaggaggg gatccgcttc cgcggc
//
LOCUS       DASVJL010000331         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_148824, whole genome shotgun sequence.
ACCESSION   DASVJL010000331 DASVJL010000000
VERSION     DASVJL010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148824"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VF357_06990"
     CDS             complement(<1..291)
                     /locus_tag="VF357_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523930.1"
                     /translation="MTRRLASLWRFVRPQRGILAATFALSLLATAASLYAPFLSKRLV
                     DDVILRGNWAALPPLLLTMVLYAAAGMVLGGVSSYLYTRGSSKILVAMRVALF"
     gene            complement(288..1310)
                     /locus_tag="VF357_06995"
     CDS             complement(288..1310)
                     /locus_tag="VF357_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX7523931.1"
                     /translation="MKLTVPISNPDEVEMLAENGAGEFFCGYVPPEWLERYGGAFWLN
                     RRGPVVGNLVQRDDVSLLTERSHAFGIPVHVTFNAPYYTPEQQEFLLPVIGRLAGESG
                     VDGVIVSDVGFLVALRRAFPELSIHASSVMATLNPGMVDFIEELGAQRVIFPRSLGVA
                     DIEALCDTAAGRLETEAFVLNEGCVFEEGYCMTTHRSVGALCVQLPGVPHRVLPLGGE
                     SAESLAGEERERARELMGAFKDWVWFQNGCGNSMNSSGIPNGPCGLCALFELSRTGVT
                     SLKISGRGASSYRKLVSLQMVRAVLDLVERGAPREEAARKAMALRNTPKFCEARYMCY
                     YREEAS"
     gene            complement(1314..2480)
                     /locus_tag="VF357_07000"
     CDS             complement(1314..2480)
                     /locus_tag="VF357_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523932.1"
                     /translation="MLLTRDHREVSPRDGAGCGVEVAISFPFADGQGWPSAEAGRDAP
                     ASIASAGPLHAVLRGEASLEEVLRDGHGWPSAEAGRDAPSSVASADGQLAEALLRHLP
                     GAAPSRVYVGNETCERLLPTPRAIVSWVASAREGRLAVSLVLPPLSRDGLAKAEEAVR
                     ALDGVEESEVVANDWGTVHRLRSRYPGLTIVLGRLTHKMLRDPRLADYFDSPQAPMAA
                     RAALCRSGELAPGFRTLMERYGIGRREVDPFLQPLEAEEWENRSEKLSVHFPYLFVTM
                     GRACLLGAMHREPDGKFVPGAPCRSECRKYAVEFRLPVPGGNGAEKNLLSLGNAYYHA
                     VPLSVTERILGRLPFHRQVDRIVVTVPGRGYRTRGIPARPGTRPSMAVEGEGTS"
     gene            complement(2494..>3197)
                     /locus_tag="VF357_07005"
     CDS             complement(2494..>3197)
                     /locus_tag="VF357_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523933.1"
                     /translation="RQRWLPDEVVDMTPAVIVFDLATDPPKVVKEIEIPRGCTNLFFG
                     YDGSEFYVTGRDVFVYDPMTGKQVNFLGLAHPTTTGVDPQISLNIWRLHDQSGMAMIL
                     VGSQATVNNLMYQGYYTIDLRKKSTEGSMKLVTDIGPLYNQFSAVVSPDRKYYYMVLN
                     KVEKRDFATNRLLATADVDKSYYTIQISSDGKKVYLGGGGDSILIYDTETMKLLKKLD
                     TPGDAVLTHFRVQSR"
BASE COUNT          552 a         1084 c         1043 g          518 t
ORIGIN      
        1 gaagagggcg acccgcatgg cgacgaggat cttcgacgac ccccgcgtgt agagatagct
       61 gctgacgcct ccgaggacca tccccgcggc ggcgtaaaga accatggtga ggagaagggg
      121 gggcagcgcc gcccagttcc cacggaggat gacatcgtcc accagcctct tcgacaggaa
      181 cggggcgtac agggaagccg ccgtcgcgag aagggacagg gcgaaggttg cggcgagaat
      241 cccccgctgg ggtcgcacga atctccacaa agaggccagc cgccttgtca tgatgcctcc
      301 tcccggtagt aacacatgta gcgtgcctcg cagaatttcg gcgtgttccg gagcgccatc
      361 gccttgcgcg ccgcctcttc ccggggggcc ccccgctcca ccaggtcgag gaccgcgcga
      421 accatctgga ggctcacgag cttccggtag ctggaggcgc cgcgtccgga gatcttgagg
      481 gaggtcaccc ccgtgcggga gagctcgaag agcgcgcaga gcccgcaggg gccgttcggg
      541 atcccgctgg aattcatgga gttgccgcag ccgttctgga accagaccca atccttgaac
      601 gcgcccatca gctcccgcgc ccgttctctt tcctccccgg ccagggattc cgcggactct
      661 ccccccaacg gaaggacacg gtgcgggacg cccgggagct gtacgcaaag ggcgccgacg
      721 ctccggtgcg tggtcatgca gtacccttcc tcgaacacgc acccttcgtt caggacgaac
      781 gcctccgtct ccagccggcc cgccgccgtg tcgcaaaggg cctcgatgtc cgccacaccg
      841 aggctgcggg ggaagatcac ccgctgggcc cccagctcct cgatgaaatc gaccatgccg
      901 gggttgagcg tcgccatcac cgagctggcg tggatggaga gttccgggaa ggcgcgacga
      961 agcgcgacga ggaaccccac gtcggagacg atgacgccgt cgactccgga ctcccccgcg
     1021 agccggccga tcacgggcag gaggaactcc tgctgttccg gcgtgtagta gggggcgtta
     1081 aatgtcacgt ggacggggat gccgaaggca tgggaacgct cggtcaggag ggagacgtcg
     1141 tcccgttgaa caaggttccc caccaccggc cctctccgat tgagccagaa ggcgccaccg
     1201 taccgctcga gccactcggg ggggacataa ccgcagaaga actcgcccgc cccgttctcg
     1261 gcgagcatct ccacctcgtc gggattgctg atcggcacgg taagcttcat ctcttagctc
     1321 gttccttcgc cttcgacggc catggatggc ctagtgccgg ggcgggcagg gatgccccgg
     1381 gttctgtagc cccggccggg aacggtgacg acgatccggt ccacttgccg atggaacggt
     1441 aaacgtccca ggattctctc ggtgacggat agcggcacgg cgtggtaata cgcgttcccg
     1501 aggctcagga gatttttctc cgccccgttt cccccgggaa cgggaaggcg gaactcaacg
     1561 gcgtacttcc ggcactccga cctgcaagga gcgccgggga cgaattttcc gtcgggttcc
     1621 cggtgcattg ctcccagcag gcaggcgcga cccatggtga cgaagaggta cgggaagtga
     1681 accgacaact tctcggaccg gttctcccac tcttccgcct cgagaggctg gaggaacggg
     1741 tcgacttccc tgcgtccgat accgtaccgt tccatcaggg tccggaaccc cggggcgagt
     1801 tcccccgacc ggcagagcgc cgcccgggcc gccatcggcg cctgcgggga atcgaagtag
     1861 tccgcgagcc ggggatcgcg gagcatcttg tgcgtcaggc gtcccaggac gatcgtgagt
     1921 cccgggtagc gggacctcag gcggtggacc gtcccccagt cgttcgcgac gacctcggac
     1981 tcttccacgc catccaacgc ccggaccgcc tcctccgcct tcgcgagacc gtcccgcgag
     2041 agcggcggca ggacgaggga cacggcgagt cgcccctccc gcgccgacgc gacccaggag
     2101 acgatcgccc gcggcgtcgg caggagccgt tcgcacgtct cgttgccgac gtacacccgc
     2161 gacggcgcgg ctccggggag gtgccggagg agcgcctccg cgagttggcc gtcggccgag
     2221 gctacggaag acggggcatc cctgccggcc tcggcactag gccatccatg gccgtcgcgc
     2281 aacacctcct ccagggatgc ttccccgcga aggaccgcat gaagcgggcc ggccgaggcg
     2341 atagaagccg gggcatccct gcctgcctcg gcactaggcc atccctggcc gtcggcgaag
     2401 gggaaggaga tcgccacctc cacgccgcac cccgcaccgt cccgaggaga cacctcccgg
     2461 tggtcccggg tcaggagcaa gcggccgcgc cccctaccgg ctctggaccc ggaagtgcgt
     2521 gaggaccgcg tcccccgggg tgtcgagctt cttcagcagc ttcatcgttt ccgtgtcgta
     2581 gatcaggatc gagtcgccgc cgccgcccag gtacaccttc ttcccgtcgg agctgatctg
     2641 aatcgtgtag tagctcttgt ccacgtcggc ggttgccagc agccgattcg tcgcaaagtc
     2701 cctcttctcc accttgttga gcaccatgta gtaatacttc cggtcggggg agacgaccgc
     2761 ggagaactgg ttgtagaggg ggccgatgtc ggtcacgagt ttcatcgacc cttccgtgct
     2821 cttcttccga aggtcgatcg tgtaataccc ctggtacatg aggttgttca ccgttgcctg
     2881 gctgccgacc aggatcatcg ccatcccgga ctggtcgtgg agccgccaga tgttcagcga
     2941 gatctgcggg tcgacgccgg tcgtcgtcgg gtgcgcaagc ccgaggaagt tcacctgctt
     3001 ccccgtcatg gggtcgtaga cgaacacatc gcgtcccgtg acgtagaact cgctcccgtc
     3061 gtacccgaag aagaggttcg tgcagccccg cgggatctcg atctccttga cgaccttcgg
     3121 gggatcggtc gcaaggtcga acacgatcac cgcgggcgtc atgtcgacga cctcgtcggg
     3181 gagccaacgc tgccgca
//
LOCUS       DASVJL010000332         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1838368, whole genome shotgun sequence.
ACCESSION   DASVJL010000332 DASVJL010000000
VERSION     DASVJL010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838368"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /gene="tsf"
                     /locus_tag="VF357_07010"
     CDS             <1..781
                     /gene="tsf"
                     /locus_tag="VF357_07010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX7523934.1"
                     /translation="AAKKSSRIASEGVVYAYVEGNSGALVEVNCETDFVARTDNFVGL
                     AKEVAALVNAKAPRDVEEALTLPAGGGSLGEKLVETVAKIGEKVSFRRFARLETPAGL
                     PGMVVPYIHAGGKIGVLVAVSGADRSNAAAAALAKDLAMQVAAANPTFVGRNDVPAPV
                     IEREKTIYREQARVSGKPDKILDKIADGKLQKYFGDFCLVEQAFIKDPDRKVSQLLAD
                     AGKDAGAPITVCAFARFQVGEGMEKRSDDLAAEVAKQLGQG"
     gene            813..1538
                     /gene="pyrH"
                     /locus_tag="VF357_07015"
     CDS             813..1538
                     /gene="pyrH"
                     /locus_tag="VF357_07015"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX7523935.1"
                     /translation="MKKPSYRRILLKLSGEAFMGKQAYGIDDAILTGLSTEIREVIGL
                     GVQVALVVGGGNIFRGIGTSRDFGIDRASADYMGMLATVINSLALQDVLERTGVMTRV
                     LSAIEMRTIAEPYIRRRALRHLEKGRVVIFAAGTGNPYFTTDTAAALRAMEINADAIL
                     KATKVDGVYDRDPMLDPKAKKYSRLTYLDVLRKNLKVMDATAISLCMDNNLPIVVFNL
                     TERGNILKAVLGEKIGTVVHGGK"
     gene            1538..>1752
                     /locus_tag="VF357_07020"
     CDS             1538..>1752
                     /locus_tag="VF357_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX7523936.1"
                     /translation="MEALVKDTAARMERSIEAFRKELGKVRTGRASFSLLDGVKVDYY
                     GTPTPLQQVGTLSVPESRLITVTPWDT"
BASE COUNT          322 a          550 c          624 g          256 t
ORIGIN      
        1 ggccgcgaag aagtcttcgc ggatcgcctc cgagggggtc gtctacgcct acgtggaggg
       61 gaactccggg gcgctggtcg aggtgaactg cgagaccgac ttcgtggcca ggaccgacaa
      121 cttcgtcggg ctggcgaagg aggtcgccgc gctcgtcaac gccaaggcgc cgcgggacgt
      181 cgaagaagcc ctgaccttgc cggccggcgg cgggagcctc ggggaaaagc tggtggagac
      241 ggtcgcgaag atcggcgaga aggtctcctt tcgccggttc gcgcgcctcg agacgccggc
      301 ggggttgccc ggcatggtcg tcccgtacat ccatgccggg gggaagatcg gcgtgctcgt
      361 ggcggtatcg ggtgcggatc gatcgaacgc cgccgcggcc gcgctggcaa aggaccttgc
      421 gatgcaggtg gcggccgcca acccgacgtt cgtcggcagg aacgatgtcc ccgcaccggt
      481 catcgagcgc gagaagacga tctaccgcga gcaggccagg gtttcgggga aacccgacaa
      541 gatcctggac aagatcgccg acgggaagct gcagaagtat ttcggcgatt tctgcctcgt
      601 cgagcaggcg ttcatcaagg acccggaccg gaaggtctct cagctcctcg cggacgcggg
      661 gaaggacgcc ggcgccccga tcacggtgtg cgccttcgcc cggttccagg tcggcgaggg
      721 gatggagaag cggtcggacg acctggccgc cgaggtggcg aagcagctcg ggcaggggtg
      781 atcgatccga cccgccgccc tgcggaggtt gcgtgaagaa accgtcgtac cgccggatcc
      841 tgctgaagct gtcgggggaa gcctttatgg ggaagcaggc gtacgggatc gacgacgcga
      901 tcctcaccgg cctgtcgacc gagatccggg aagtcatcgg actcggcgtc caggtggccc
      961 tcgtcgtcgg cggagggaac atcttccggg ggatcggcac cagccgggac ttcggcatcg
     1021 accgcgcctc cgcggactac atggggatgc tggcaacggt catcaacagc ctcgccctgc
     1081 aggacgtcct cgagcggacc ggcgtcatga cccgcgtgct gagcgcgatc gagatgcgca
     1141 cgatcgccga gccgtacatc cgccggcgcg ccctgcgcca tcttgaaaag gggcgggtgg
     1201 tgatcttcgc ggcggggacc gggaacccgt atttcacgac ggacaccgcg gcggcgctgc
     1261 gggcgatgga aatcaacgcc gacgccatcc tcaaggccac caaggtcgac ggcgtgtacg
     1321 atcgcgaccc gatgctcgac ccgaaggcga agaagtactc ccgcctgacc tacctcgacg
     1381 tcctccggaa gaacctcaag gtcatggacg ccacggccat ctccctgtgc atggacaaca
     1441 acctccccat cgtcgtgttc aatttgacgg agagggggaa catcctgaag gcggtgctgg
     1501 gcgagaagat cggcaccgtg gtccacggag ggaagtgatg gaagcgctgg tgaaagacac
     1561 cgccgcccgc atggagcgga gcatcgaagc gttccggaag gagctgggga aggtgcgcac
     1621 ggggcgcgcc tccttctcgc tgctggacgg ggtcaaggtg gactattacg gcacccccac
     1681 gccgctccag caggtgggga ccctctcggt cccggagagc cgcctgatca ccgtcacccc
     1741 ctgggacacc aa
//
LOCUS       DASVJL010000333         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1087733, whole genome shotgun sequence.
ACCESSION   DASVJL010000333 DASVJL010000000
VERSION     DASVJL010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087733"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VF357_07025"
     CDS             complement(<1..331)
                     /locus_tag="VF357_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523937.1"
                     /translation="MKPHARVTHWLALATAVFLLPACMSAPQIQPGAAAPATPGAQAV
                     SAPPAQSGASALSYGTVTATVVKGKTTQMDLIQWFGGPNISTVDSDGVETWLYERSVS
                     QTDVASKS"
     gene            560..1588
                     /locus_tag="VF357_07030"
     CDS             560..1588
                     /locus_tag="VF357_07030"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7523938.1"
                     /translation="MKAKSVLPVLLSVLLLAFLVAPGTGRTAGTITLRYANFPPSATF
                     PCVQMERWAKEVEKRTHGRVKVQTFPGGTLLAAKNIFDGVISGMADIGNFAMSYQPGR
                     FPVSEAVDLPMGFTSARVASLTLFDLIEKYKPKEFAQVKVLTLFTCPPTNFMTKAPVK
                     SLADLKGMELRVAGTNAEVVKRLGGIPVAMPQSETPEAIQKGVVKGMVSSMEILKDFK
                     FAAYTPYATVANLPVVSFAVVMNKEKWDSLPAEVKDVLEQMRREQADWTGKYVDDHVT
                     EALAWSKASYQHQLFELSAADHDKVASLLKPMTDDYVKRTGALGLPASQIVADTLGLK
                     KKYEQKYR"
     gene            1601..2086
                     /locus_tag="VF357_07035"
     CDS             1601..2086
                     /locus_tag="VF357_07035"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7523939.1"
                     /translation="MGLERVSELLRRVLMIAAGVSLLALTLLATMNVALRIFQVPVGG
                     TYEVVSFLGAIVTAGALGYTQKRKDHIVVDILSDKFPAKVKRVLDQVSYALLLVFFSI
                     VSWQTFVYARRLMQTGEMSETLRIAYYPFVFLVSLGFAALALTVFLDLIETVWTREEK
                     K"
     gene            2083..>2382
                     /locus_tag="VF357_07040"
     CDS             2083..>2382
                     /locus_tag="VF357_07040"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7523940.1"
                     /translation="MSGPIVGVYGIVVMFFILFVLRVPAAFTMALVGFVGIAQVISFE
                     AAFSIVGTEMWNIFSSYGLTVIPLFILVGEIVHYAGYNFSLYDATYKWFGHFRGGL"
BASE COUNT          426 a          735 c          749 g          472 t
ORIGIN      
        1 ggcttttgct ggcgacgtcc gtctggctga ccgaacgctc atagagccag gtttccaccc
       61 cgtcgctgtc cacggtcgag atgttgggtc cgccgaacca ctggatgaga tccatctggg
      121 tggttttccc cttgacgacg gtcgctgtta cggtgccgta ggagagggcg ctcgcgccgc
      181 tctgggcggg cggggccgac acggcctgcg cgccgggggt cgcgggggcg gcggccccgg
      241 gctgaatctg cggggcggac atgcaggcag gcaggaggaa cacggcggtc gcaagcgcga
      301 gccagtgggt aacgcgggcg tggggcttca tcggcggggc tcctttttct tggcgtaaat
      361 ttaaaacatc atttcagtat ttccacctac ggtcaatccg attccactta tttcgtcatc
      421 gctggaaggg aaacgtcgtt tccgcaacgg gaaatacaaa taccaggttg ccgcatgagg
      481 gaggattcgg ttattatggg acaattttcc ccccggacaa tgcagacgga ttcctaatcc
      541 atttcccagg gaggcgctcg tgaaagcgaa atcggttctg cctgtactcc tgtcggtcct
      601 tctccttgcg ttccttgtcg cccccgggac ggggcggacc gccgggacga tcacgctccg
      661 ctatgccaac ttcccgccgt cggccacatt tccctgcgtc cagatggagc gctgggcgaa
      721 ggaagtggag aagaggaccc acggcagggt gaaggtccag acgttcccgg gggggacgct
      781 cctcgcggcc aagaacatct tcgacggcgt catctcgggg atggccgaca tcgggaactt
      841 cgcgatgagc tatcaaccgg gccggttccc cgtttccgag gccgtggatc ttccgatggg
      901 gttcacgagc gcccgcgtcg cgagccttac cctgttcgac ctgatcgaga agtacaagcc
      961 gaaggagttc gcgcaggtca aggtgctcac actcttcacc tgccccccga ccaacttcat
     1021 gacgaaggcg ccggtgaagt cgctggccga cctcaagggg atggagcttc gggtcgccgg
     1081 cacgaacgcc gaggttgtga aacggctggg cgggatcccc gtcgcgatgc cgcagtccga
     1141 gacgcccgag gcgatccaga agggggttgt gaaggggatg gtttcgtcga tggagatcct
     1201 caaggacttc aagttcgccg cctacacgcc gtacgccacg gtcgcgaacc ttcccgtcgt
     1261 ctccttcgcc gtcgtgatga acaaggagaa gtgggactcc ctcccggcgg aggtgaagga
     1321 cgtcctcgag cagatgcgcc gcgagcaggc ggattggacc gggaagtacg tggacgacca
     1381 cgtgaccgag gccctcgcct ggtcgaaagc gagttaccag caccagctct tcgagctctc
     1441 cgcggcggac cacgacaagg tcgcctccct cctcaagccg atgacggacg attacgtcaa
     1501 aaggaccggc gcgctcggcc tgccggccag ccagatcgtc gccgacaccc tcggcttgaa
     1561 gaaaaagtac gagcagaagt accggtagcc ggattcctcg atgggactgg aacgggtttc
     1621 cgaactgctt cggagggtcc tgatgatcgc cgcgggcgtc tcgctcctgg cgctcacctt
     1681 gctggcgacg atgaacgtcg ccctgaggat attccaggtc ccggtgggcg ggacgtatga
     1741 ggtcgtctcc ttcctcggcg cgatcgtcac cgccggagcc ctcggctata cacagaaaag
     1801 gaaggaccac atcgtcgtgg acatcctctc ggacaagttc ccagcgaagg taaagcgggt
     1861 tctcgaccag gtgagctacg ccctcctcct cgtcttcttc tccatcgtct cctggcagac
     1921 cttcgtgtac gcgaggaggc tgatgcagac gggcgagatg tccgaaacgc tgaggatcgc
     1981 ctattacccc ttcgtgttcc tcgtcagcct cgggttcgcc gctctcgccc tgacggtttt
     2041 cctcgacctg atcgaaaccg tctggacccg cgaggagaag aaatgagcgg gcccatcgtc
     2101 ggcgtgtacg ggatcgtcgt catgtttttc atcctgttcg tcctgcgggt ccccgccgcc
     2161 ttcaccatgg cactggtcgg gttcgtcggg atcgcccagg tgatctcctt cgaagcggct
     2221 ttctcgatcg tcggcaccga gatgtggaac atcttctcca gctacgggct gacggtgatc
     2281 ccgctcttca tcctggtcgg cgagatcgtc cattacgccg ggtacaattt cagcctctac
     2341 gacgccacct acaaatggtt cgggcatttc cgcggcgggc tc
//
LOCUS       DASVJL010000334         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_711409, whole genome shotgun sequence.
ACCESSION   DASVJL010000334 DASVJL010000000
VERSION     DASVJL010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711409"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VF357_07045"
     CDS             <1..610
                     /locus_tag="VF357_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7523941.1"
                     /translation="GRSGRVVASDLTPSMLREARGLAAEHRAGNVLFTAADAEALPFR
                     DATFDRVTCRIAPHHFPDLRTAMAEMARVIRPGGRIGIIDSTVPGEPALDAFLNGVEK
                     VRDPSHVRSYRVEEWLELLAGAGFLLLQAASIWKTHAFPEWVARTGLPKEVQWEVETI
                     FLSAFPLAQETFRIRTEGGRVVSYSDEKAIFVAKKPGGAPAP"
     gene            717..2816
                     /locus_tag="VF357_07050"
     CDS             717..2816
                     /locus_tag="VF357_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX7523942.1"
                     /translation="MDCPDEEKEIRAALSRLPAVESLTFHLFSRQVEVRHRGEVEPVL
                     DALRRIGMEGHPLDDALRQAKLPSSDKGPRNTFLFSGAALLLGAGSRILFPGESLVRL
                     AFLASILLGGVPVAIRGFREVRNRSLGMNALMTISIGGAALLGDWAEAAVVVALFALA
                     NTLEARSLDRARRATADLFASSPERAVVRIGGPGGEERIVPAEEVRPGDTLIVRPGER
                     VPVDAVIRRGASDLNESVLTGESVPVEKKEGEELFAGTVNGRGLLISEATRPLSESTY
                     ARILRRVEEAQAEKAPVQTFMERFAAAYTPAVLCAAVLVAAVPPLLGRGLALDWAYRA
                     LVMLVIACPCAIVLAAPVVTLSALTRATREGILVKGARHLEAMGTIRAVAFDKTGTLT
                     RGRLQVTRVRAADGWTEEEVLRLAGAVEAASAHPAALAIRREALLRGVGTSARGTLAR
                     TFEPVEGQGVAAEVEGRKIYVGNRRMFETMGFGGNALDRLLENGGSAAPSIAIVGMRD
                     TLAGAVEMEDELRPEAPEAIRALRDLGVAHIVLLTGDNAGAARKAGASAGIDIVEHGL
                     LPEDKLARIRDLVASHGSAAMVGDGVNDAPALALATVGVAIAAAGSPAAMETADVALM
                     TGDLRRIPSAVVLGRRMVALVRQNVAASLAIKAVFLAMAVAGYASLWMAVLADMGTTL
                     LVIFNGLRLLRARTAHH"
BASE COUNT          417 a          994 c         1047 g          468 t
ORIGIN      
        1 cgggcgctcc ggacgggtgg tggcgtcgga cctcacccct tccatgctgc gggaggcgcg
       61 gggtctggcc gcagagcacc gggccgggaa cgtcctcttc acggccgccg acgccgaggc
      121 gctcccgttc cgggacgcta ccttcgaccg ggtgacgtgc cgcatcgcgc cgcatcattt
      181 ccccgacctt cggaccgcca tggccgaaat ggcccgggtg atccgccccg gcgggcggat
      241 cggcatcatc gacagcacgg tccccggcga gccggccctc gacgccttcc tgaacggcgt
      301 cgagaaggtg cgcgacccgt cgcacgtgcg cagctaccgc gtagaggagt ggctcgagct
      361 cctcgcgggg gcgggatttc ttctcctgca ggccgcctcc atctggaaaa cccacgcctt
      421 ccccgaatgg gtggcgcgga caggccttcc caaggaggtg caatgggagg tcgagacgat
      481 cttcctctcc gcctttccgc tcgcgcagga gacgttccgc atccgcaccg aggggggacg
      541 ggtcgtctcc tattccgacg agaaagcgat cttcgtcgcg aagaaacccg ggggggctcc
      601 cgccccctga cgtcgctccc tcgccgctac gcaacccgcg gcgtcaggcg gccacggacc
      661 tcaagtcgat gcgaactccc gacgacctaa cacgcacccg gatcttcgta cccgccatgg
      721 attgtcccga cgaggagaag gagatccgcg ccgccctctc gaggctcccc gccgtcgagt
      781 cgctgacgtt ccatctcttc tcacggcaag tggaggtgcg tcaccgcgga gaggtggagc
      841 cggtcctcga cgccctccgc cggatcggga tggagggtca tccgctggac gacgcgctgc
      901 gccaggcgaa actcccctcg tcggacaagg ggccccggaa caccttcctc ttttccggcg
      961 cagcccttct tctcggcgca ggatcacgga ttctctttcc cggggaatcc ctggtccgcc
     1021 tcgcctttct tgcgtcgatc ctcctcggcg gcgtgccggt ggcgatacgg gggttccggg
     1081 aggtgcggaa ccggtccctc gggatgaacg ccctgatgac gatctcgatc ggcggcgcgg
     1141 ccctcctcgg ggattgggcg gaggcggcgg tggtggtggc cctcttcgcc ctcgcgaata
     1201 cgctggaggc gcgcagcctc gatcgcgccc gccgcgccac cgccgacctg ttcgcctcct
     1261 cccccgaacg ggcggttgtc cgcatcggcg gcccgggagg cgaagaacga atcgtccccg
     1321 ccgaggaggt ccgtcccgga gacacgctga tcgttcgacc cggagagcgc gtccccgtgg
     1381 acgccgtgat ccgccgaggc gcctcggacc tcaacgagtc ggttctcacg ggcgagtcgg
     1441 tccccgtgga aaagaaagag ggggaagagc tgtttgccgg cacagtgaac ggacggggcc
     1501 tgctgatctc cgaggcgacg cgtccgttgt ccgaatccac ctacgcccgg atcctgcgcc
     1561 gggtggagga ggcgcaggcg gaaaaagctc ccgtgcagac gttcatggag cggttcgccg
     1621 ccgcgtacac cccggcggtt ctgtgcgctg ccgtcctcgt ggccgcggtc cctcccctgc
     1681 tcggccgggg tctcgctctc gactgggcgt acagggcgct cgtgatgctc gtcatcgcct
     1741 gcccgtgcgc gatcgtcctt gccgctcccg tggtcacgct ttccgccctc acgcgcgcga
     1801 cccgggaggg gatcctcgtc aagggtgcca ggcaccttga ggcgatgggg acgatccggg
     1861 cggtggcgtt cgacaagacg ggaacgctca cgcggggaag gctccaggtg acccgggtcc
     1921 gggcggcgga cgggtggacg gaagaagagg ttctccggct ggcgggtgcg gtcgaggcgg
     1981 cctcggcgca ccccgccgca ctggcgatcc gtcgggaggc tttgcttcgg ggcgtcggaa
     2041 cgtcggcgcg aggaaccctc gcccggacat tcgagcccgt ggaagggcaa ggcgtcgccg
     2101 ccgaagtgga ggggcggaag atctacgtcg gaaaccggag gatgttcgag acgatggggt
     2161 ttggcggcaa cgcgctcgac cggctcctcg aaaacggggg gagcgcggcg ccgtcgatcg
     2221 cgattgtcgg aatgcgggac acccttgccg gggcggtgga gatggaagac gagcttcgtc
     2281 cggaggcgcc ggaggcgatc cgggcgttgc gggatctcgg cgttgcgcac atcgtcctgc
     2341 tcacggggga caacgcggga gcggccagga aagccggagc gtccgccggc atcgacatcg
     2401 tggagcacgg cctgctgccg gaggacaagc ttgcgcgcat ccgggacctc gtcgcctccc
     2461 acgggtcggc ggcgatggtg ggcgacggcg tgaacgacgc cccggcgctg gccctggcca
     2521 ccgtcggcgt ggcgatcgcg gcggcgggct ccccggcggc gatggagacg gcggacgtgg
     2581 cgctcatgac gggggatctc cgaaggatcc cctcggcagt cgtcctcggg cggcgcatgg
     2641 tggccctggt gcggcagaac gtggctgcct ccctcgccat caaagcggtg ttcctcgcga
     2701 tggcggtagc cgggtacgcc tccctgtgga tggcggtgct ggccgacatg gggaccacgc
     2761 tgctggtgat cttcaacggg ttgcgcctgt tgcgcgcccg tacggcccat cattgagggt
     2821 ttgatggaac gcgcgaagct gcaccggaac gtcgtcgcca tcgggctggt gagcctgctc
     2881 accgatctgt cgagcgagat gatctacccg ctccttccgg tcttcc
//
LOCUS       DASVJL010000335         3805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1557154, whole genome shotgun sequence.
ACCESSION   DASVJL010000335 DASVJL010000000
VERSION     DASVJL010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557154"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /gene="hemB"
                     /locus_tag="VF357_07055"
     CDS             <1..572
                     /gene="hemB"
                     /locus_tag="VF357_07055"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX7523943.1"
                     /translation="TEVDNDATLEILAKSAVSHAKAGADIVAPSDMMDGRVGAIRKAL
                     DRGGFSQIPIMSYAAKYAGGFYGPFRDAAGSAPSFGDRRSYQMDPPNAREALREVALD
                     VAEGADIVMVKPALAYLDIIYRVRQAFNLPVAAYNVSGEYAMVKAAAKLGWIDGDRVA
                     MEILVSIKRAGADLIITYAAKEAARAIAG"
     gene            577..783
                     /locus_tag="VF357_07060"
     CDS             577..783
                     /locus_tag="VF357_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523944.1"
                     /translation="MSVPPTGQAPWRVVELADVTDRTIEEALNAAAGDGWRFESVHFV
                     TQPGNRRPMMAFLFFTRDALPQGS"
     gene            787..1272
                     /locus_tag="VF357_07065"
     CDS             787..1272
                     /locus_tag="VF357_07065"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7523945.1"
                     /translation="MSLRWEEQREFFRDARRARYLARTVGKAADLIAAMSLWHIPGAA
                     GVFAALFYILMCDGFPGGRSLGKWLTGLKVVRIDRDGMDFTASLMRNLPVAAPFLLYL
                     FPVIGAFLAYTVGIAILLIETYLGFYDPDGQRAGDTFAETLVVEYRQGSDGVPLSDRR
                     A"
     gene            1241..>3805
                     /gene="polA"
                     /locus_tag="VF357_07070"
     CDS             1241..>3805
                     /gene="polA"
                     /locus_tag="VF357_07070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX7523946.1"
                     /translation="MASLYLIDGHNVLYRTFFGVPRLTAPDGTPTNVVLGVARILLKI
                     LREERPDAIVAVFDSREPTPRHAIYPEYKANRLKTPEDLAVQIPVVDEMIDALGVCRL
                     SVAGAEADDIIGTLSRRAEERGIDVVIVSSDKDMYQLVSPRVKVRDGLKEHAVGEAQV
                     EEVFGVPPGKVADLLALAGDPSDNVPGVPGIGEKTASELIRDFGSLDGVLAHPERLKG
                     ARREKIEKGADAARLALRLVTIDRDIPIREDWSEFTPRGIDASRVAPLFRRLGFRKLL
                     EELDLGKETPSERKGDFPKSVGWKRAASVEEFLHAMGPGDDVTSAGLAYDGDRETVVG
                     IAVEGKGVHLLPADASARAARALSARGATIYLHDGKALYRRDVGAAPGEDPRLFDTQV
                     AGYLLEPEEGTPSFPKLRARFLPASLAEAEGESPSARAADRAGAMLALGKVLEDRLAD
                     AALLDVFRGIDMPLLPVLHRIEEKGIRIDPGIFAELSEGLARDISAIERKVAAAAGTD
                     FNINSPKQLAFLLFEKLGLPPVKKTKTGYSTDVDVLERLKDLHEIPSLVLEYRTVAKI
                     RSTYVDVLPGRIDPRDGRIHTTLHQTQAATGRLSSSDPNLQNIPIRTELGRRIRAGFV
                     AENGNLFVGADYSQVELRLLAHLSGDAELIRRFRQGDDIHTSTAAAVFGVNPSAVTPE
                     LRRRAKVINFGILYGMSPFGLSRELGIGGKEAKTYIDHYFDRYPGVKEYIEGLKATAR
                     KDGYVITIMGRRRFLKDINSRNKVLREAAERMAINTPIQGSAADLIKMAMIRVDREFR
                     EAGMEARLILQVHDELIVEAPEMEVAGSERILKEAMEGVAKLSVPLTVSVSRGKNWG"
BASE COUNT          634 a         1243 c         1317 g          611 t
ORIGIN      
        1 ggaccgaggt cgacaacgac gcgacgctcg agatcctcgc gaagagcgcc gtttcgcacg
       61 cgaaggcggg ggcggacatc gtcgccccct ccgacatgat ggacggacgc gtcggcgcga
      121 tccggaaggc gctggatcgc ggcggatttt cgcagatccc catcatgtcg tacgcggcga
      181 agtacgctgg aggattctac ggcccgttcc gggacgccgc ggggagcgcc cccagcttcg
      241 gggaccggcg ctcctatcag atggacccgc cgaacgcgcg ggaagcgctt cgggaagtcg
      301 ccctcgacgt ggcggaggga gccgacatcg tgatggtcaa gcccgcactg gcgtacctcg
      361 acatcatcta ccgcgtccgc caggccttca acctcccggt cgcggcgtac aacgtgagcg
      421 gggagtacgc gatggtgaag gcggcggcga agctcgggtg gatcgacggg gaccgcgtcg
      481 cgatggagat cctcgtttcg atcaagcggg ccggcgccga cctgatcatc acctacgccg
      541 ccaaggaagc ggcccgcgcc atcgcgggct gacgccgtgt ccgttccgcc gacgggacag
      601 gccccttggc gcgtggtcga gctggcggac gtcaccgacc gcacgatcga ggaggcgttg
      661 aacgcggcgg cgggggacgg gtggaggttc gagtcggtcc acttcgtcac gcagcccggg
      721 aaccggcggc ccatgatggc gttcctgttt ttcacccggg acgctctccc gcaggggagc
      781 tgacccgtgt cgcttcgctg ggaggaacag cgggagttct tccgggatgc ccggcgggcg
      841 cggtacctgg cgcggacggt cgggaaggcg gccgacctga tcgccgcgat gtccctctgg
      901 cacatccccg gcgctgccgg ggtgttcgcc gccctcttct atatactgat gtgcgacggc
      961 ttcccggggg ggcgcagcct ggggaagtgg ctcaccggcc tcaaggtggt gcggatcgac
     1021 agggacggga tggatttcac ggcgtcgctg atgcgcaacc tccccgttgc cgcgcccttc
     1081 ctcctctacc tcttcccggt gatcggcgca ttcctcgcct acaccgtcgg gatcgcgatc
     1141 ctgctgatcg agacgtacct cgggttctac gaccccgatg ggcaacgggc gggcgacacc
     1201 ttcgccgaga cgctcgtcgt ggagtaccgg caggggtccg atggcgtccc tctatctgat
     1261 cgacgggcat aacgtcctct acaggacctt cttcggcgtc ccgcgcctca cggcgccgga
     1321 cggcacgccg accaacgtcg tcctcggggt tgcccggatt ctcctgaaga tcctgcggga
     1381 ggagcgcccc gacgcgatcg tcgccgtctt cgactcgcgg gagcccaccc cgcggcacgc
     1441 gatctacccg gaatacaagg cgaaccgcct gaagaccccc gaggacctcg ccgtgcagat
     1501 ccccgtggtg gacgagatga tcgacgccct cggtgtgtgt cgcctctccg tcgccggcgc
     1561 cgaggcggac gacatcatcg gcaccctgtc gcgccgcgcg gaggagcggg ggatcgacgt
     1621 ggtcatcgtc tcctccgaca aggacatgta ccagcttgtc tcgccgcgcg ttaaggtgcg
     1681 ggacgggttg aaggagcacg ccgtggggga ggcgcaggtc gaggaggtct tcggcgttcc
     1741 cccagggaag gtggccgatc tcctcgcgct ggccggagac ccgtccgaca acgttcccgg
     1801 cgttcccggg atcggggaga agacggcgtc cgagttgatc cgggacttcg gttccctcga
     1861 tggggtgctc gcccacccgg agcggttgaa aggggcccgg agggagaaga tcgagaaggg
     1921 cgccgacgcc gcgcgtctcg ccttgcgcct ggtcaccatc gaccgggaca tcccgatccg
     1981 ggaggattgg tccgagttta cccctcgcgg gatcgacgcc tcccgggtag cgcccctttt
     2041 ccgtcgcctc ggtttccgga aacttctcga ggaactcgat ctcggaaagg aaacgccttc
     2101 ggaaagaaaa ggggatttcc cgaagagcgt ggggtggaaa cgcgcggcat ccgtcgagga
     2161 gttcctccac gcaatgggcc ccggcgacga cgtcacctcg gccgggttgg cgtacgacgg
     2221 ggaccgggag acggtggtcg ggatcgccgt cgagggaaaa ggcgtccacc ttcttccggc
     2281 ggacgcatcg gcgcgcgccg cgcgggccct ctccgcccgc ggggccacca tctacctcca
     2341 cgacgggaag gcgctctacc gccgggacgt gggagcggcg ccgggggaag acccgcggct
     2401 cttcgacacg caggtggcgg ggtatctgct ggagcccgaa gaggggaccc cctccttccc
     2461 gaagctgcgc gcgcgcttcc tccccgcctc cctcgccgag gcggaagggg agtccccttc
     2521 cgcccgggcg gccgatcggg cgggcgcgat gcttgcgctc ggtaaggtgc tcgaggatcg
     2581 cctcgcggac gcggcactgc tcgatgtatt ccgggggatc gacatgcccc tcctccccgt
     2641 ccttcaccgg atcgaggaga aagggatccg gatcgatccc gggatcttcg cggaactttc
     2701 cgaggggctc gcccgcgaca tctccgccat cgaacggaag gtggcggcgg ccgcggggac
     2761 cgatttcaac atcaactccc cgaagcagtt ggctttcctc ctcttcgaaa agctgggact
     2821 tccccccgtc aagaagacga agaccggcta ctccacggac gtggacgtcc tggagcgact
     2881 gaaagacctc cacgagatcc cgtcgctggt gctcgaatac cgaaccgtcg cgaagatccg
     2941 ctccacctac gtcgacgtcc tgcctggaag gatcgacccg cgggacgggc ggatccacac
     3001 gacgctccat cagacgcagg cggccaccgg gcggctctcc tcatccgatc cgaacctgca
     3061 gaatatcccg atccgaaccg agctcggccg ccggatccgg gccgggttcg tcgcggagaa
     3121 tgggaacctg ttcgtcggtg cggactactc ccaggtggaa ctgcgcctgc tcgcgcacct
     3181 gtccggggac gcggagctga tccgccggtt ccggcagggg gacgatatcc atacctctac
     3241 agcggccgcc gtcttcggcg tcaacccgtc cgccgtcacc ccggagctgc ggcgccgggc
     3301 gaaggtgatc aatttcggaa tcctctacgg catgagcccc ttcggtctgt cacgcgagct
     3361 ggggatcggt gggaaggagg cgaagaccta catcgaccac tatttcgacc ggtaccctgg
     3421 agtgaaggaa tacattgaag gattgaaggc aacagcccga aaagatggat atgttattac
     3481 catcatgggg cgccgccgtt tcctcaagga catcaattcc cggaacaagg tgctgcggga
     3541 ggccgcggag cggatggcga tcaacacgcc gatccagggg agcgccgccg atctgatcaa
     3601 gatggcgatg atccgggtcg accgggagtt ccgcgaggcg gggatggaag cgcggttgat
     3661 cctccaggtt cacgacgaat tgatcgtcga agcgccggag atggaggtcg ccggttccga
     3721 gcggatcctg aaggaggcga tggagggggt ggcgaagctg tcggttcccc tgaccgtctc
     3781 ggtgagccgg gggaagaact ggggg
//
LOCUS       DASVJL010000336         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_430521, whole genome shotgun sequence.
ACCESSION   DASVJL010000336 DASVJL010000000
VERSION     DASVJL010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430521"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1200)
                     /gene="tal"
                     /locus_tag="VF357_07075"
     CDS             complement(64..1200)
                     /gene="tal"
                     /locus_tag="VF357_07075"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX7523947.1"
                     /translation="MKTNPLVALGRVGQSPWIDYIHRGMIASGELARRIAEDGIRGVT
                     SNPTIFEKAVSSGRDYDDQIRALARAGATLLDAYKSIVTDDIRAAAGVLRPVYDASRG
                     DDGYVSLEVDPDLARDTKATIARARELFDAVGRPNVMIKIPGTKEGLPAVEETIAAGI
                     PVNVTLIFSVRRYEDVAAAYLRGMERWIAAGGDPRNVASVASFFVSRIDTAVDSLLLS
                     TVERWPGSPKAETALSLLGKTAVASARLAYARFLEISDTPRWRSLAALGARVQRPLWA
                     STGTKNPKYSDVKYVEELIGPDTVNTMPPQTMDAFRDHGAVADTLSGAGADAKAVLDD
                     YALMETGIEEVCARLEEEGVKSFLDSYRKLLAAVEGRLNSASGG"
     gene            complement(1197..>1825)
                     /locus_tag="VF357_07080"
     CDS             complement(1197..>1825)
                     /locus_tag="VF357_07080"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7523948.1"
                     /translation="GEDGPTHQPVEHLASLRAMPNLCVVRPADANETAAAWRMALERT
                     TGPTALVLSRQKLPVLPPSSVFRDDGVYRGAYVLEDAGDGRPDVLLIASGSEVSVALA
                     ARKLLAEEGVPARVVSMPCWERFEEQEAEYREAVLPPSVPARVSVEAGSIFGWGRYVG
                     DRGASVGIDRFGASAPAERIFRELGITPEAVRDRAKSVLAAIRNGVRT"
BASE COUNT          272 a          696 c          584 g          273 t
ORIGIN      
        1 cctgcgcctc ccgccggtag agcgcgaggc tggtcttcgt cccgccgacg tcccccgcga
       61 ggatcatccg cccgaggcgc tgttcagccg cccctcgacc gcggcgagga gcttccggta
      121 cgaatcgagg aagctcttca ccccctcctc ctccaggcgg gcgcaaacct cctcgatccc
      181 ggtttccatc aacgcgtagt cgtcgaggac cgccttcgcg tccgcgcccg cgccggacag
      241 ggtgtcggcc acggcaccgt ggtcccggaa ggcgtccatc gtctgcggcg gcatggtgtt
      301 cacggtgtcg gggccgatca gttcctcgac gtacttcacg tcggagtatt tcgggttctt
      361 cgtcccggta ctcgcccaga ggggccgctg cacccgcgcc ccaagcgccg caagggagcg
      421 ccaccgcggc gtatcggaga tctcgagaaa cctcgcatac gccagccgcg cgctggccac
      481 cgccgtcttc ccaaggaggg agagggcggt ctcggccttc ggcgagccgg gccaccgctc
      541 caccgtggaa aggagcaggg agtcgaccgc cgtgtcgatc ctcgaaacga agaaggaggc
      601 gaccgacgcg acgttccggg gatccccgcc ggcggcgatc caccgctcca tgccgcgcag
      661 gtaggcggcg gccacgtctt cgtatcgcct caccgagaag atcagcgtta cgttcaccgg
      721 gatccccgcg gcgatcgtct cctccacggc cggaagccct tccttcgtgc cggggatctt
      781 gatcatcacg ttcggccggc cgacggcgtc gaacagctcc cgcgcccggg cgatcgtcgc
      841 cttcgtgtcg cgcgccaggt ccgggtcgac ctcgagcgag acgtacccgt cgtccccccg
      901 ggaagcgtcg tacacggggc gcagcacgcc ggcggcggcc cggatgtcgt cggtgacgat
      961 cgacttgtac gcatcgagga gcgtcgcccc cgcgcgcgcg agcgcacgga tctggtcgtc
     1021 gtagtcgcgt ccggaggaga ccgccttctc gaagatcgtc gggttggagg tcaccccccg
     1081 gatgccgtcc tccgcgatcc gccgggcgag ctcgccggac gcgatcatcc cccggtggat
     1141 atagtcgatc caggggctct ggcccaccct gcccaacgca accaacggat tcgtcttcat
     1201 gtccggactc cattccggat cgcggcgagg accgatttcg cccggtcccg caccgcctcc
     1261 ggtgtgatcc ccagttcacg gaagatccgc tcggccggcg cggaagcgcc gaaccggtcg
     1321 atcccgaccg acgcgccgcg atcccccacg taccgccccc agccgaagat cgaccccgcc
     1381 tccacggaga cgcgggcggg aaccgacggt gggagcaccg cttcccggta ctccgcctcc
     1441 tgctcctcga accgttccca gcagggcatg ctcaccacgc gcgccggcac cccctcttcc
     1501 gccagcaact tccttgccgc cagcgccacg gacacctcgg accccgaagc gatcagcagg
     1561 acgtcgggac ggccgtcccc cgcgtcttcc agcacgtagg ctccgcggta cacgccgtcg
     1621 tcgcggaaca cgctcgaagg gggaaggacg gggagttttt gccgcgagag gacgagcgcc
     1681 gtgggacccg tcgtccgctc cagcgccatc cgccatgccg ccgccgtctc gttcgcatcc
     1741 gcgggccgga cgacgcaaag attcggcatc gcccgaagcg acgcgaggtg ttcgaccggc
     1801 tggtgcgtcg ggccgtcctc cccca
//
LOCUS       DASVJL010000337        11075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1368287, whole genome shotgun sequence.
ACCESSION   DASVJL010000337 DASVJL010000000
VERSION     DASVJL010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 11075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11075
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368287"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VF357_07085"
     CDS             complement(<1..951)
                     /locus_tag="VF357_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX7523949.1"
                     /translation="MDLFQKQAQPRAIQDEMRQSYLDYAMSVIVGRALPDVRDGLKPV
                     QRRILFAMHELGNEYGKPYKKSARIVGDVIGKYHPHGDNAVYDALVRMVQDFSLRYPL
                     IDGQGNFGSVDGDSAAAMRYTEVRMAKVAGELLSDLDKETVETLPNYDGSLQEPRVLP
                     SRVPNLLVNGSAGIAVGMATSIPPHNLGEVIGALLALIGNPDITIDDLMEHVPAPDFP
                     TGGILYGLDGVRDAYRTGRGSVQIRARAFIEKAKKGDRESIVVTEIPYQVNKSRLIER
                     MAELVRNKEIEEISDLRDESDRDGMRVVVELKKDAVAEVVL"
     gene            complement(1105..2772)
                     /gene="ilvD"
                     /locus_tag="VF357_07090"
     CDS             complement(1105..2772)
                     /gene="ilvD"
                     /locus_tag="VF357_07090"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7523950.1"
                     /translation="MRSDTVKKGFERAPHRSLLKATGVTDADMGKPFIAVCNSFVEII
                     PGHVHLNRVGRYVKECVREAGGVAFEFNTIGVDDGIAMGHGGMLYSLPSREIIADSVE
                     TMLKAHCFDGMICIPNCDKIVPGMLMAAMRCNIPTIFVSGGPMKAGLTKAGRVVDLIS
                     VFEGVAAHQLGNLSDEGLKELEDTGCPTCGCCSGMFTANSMNCLCEALGMALPGSASV
                     LAIDKQRGDYYREAAFRLMDLVRKDTKPRDIVTRDAFDNALALDMAMGGSTNTILHTL
                     AVAHEAGVSFDLARIEAISRKTPTLCKVSPSSQYHMEDVDRAGGIPAILSELFKVPGL
                     MKRGCLTVTGKTLEENVAIAVSKDPAVIRTLSNAYSKEGGLAVLYGNLAMEGAVVKTA
                     GVDPKMLVFEGPAVIFESQDEACEGILGGKVKAGDFVVIRYEGPKGGPGMQEMLAPTS
                     YIMGEGLGDKVAMVTDGRFSGGTRGACIGHVSPEAAAGGTIALVRKGDRIRLDIPARK
                     LDLLISDAELTERRTKWQLPPKEKSAGCLGKYAAMATSASTGAVLKR"
     gene            complement(2813..4453)
                     /locus_tag="VF357_07095"
     CDS             complement(2813..4453)
                     /locus_tag="VF357_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX7523951.1"
                     /translation="MDLSGTVARVGAICRRFRIDSLAPQLAACEELLGGGGVVDVAVL
                     GQFKAGKSSFLNGLIGETIVPVDVLPSTAVITRIGYGERERVTVCGLAGEPFEIPLGR
                     LAEFVTERGNPANEKRVAVVDVELPALASYEGIRFVDTPGLGSVFAHNTKVSREWMPR
                     IGAALVAVSVNHPLSEDDLLLLNDVSSHSPEAALLLTKADLVSGEELATVIEFTRSQA
                     TSRTGKEWRILPVSNRPGYEGMRKEVAEYLRERIAGRREEAFGEIVHHKVRALVAGCR
                     EYLNLAERAAEAADTARSDLIEALARERRGFGPVKGELRLLCNHLQAEVRTAADERFH
                     AYHGEVTGRVTEALREAMTGWKGHLGRVTREFEGWLADAMMEEMGAVSLHGEGHLAGF
                     LFRAQASVERSVRAFADRLAGEIERALGIRFEGARFDPQVEEPAHPDVRLSPTFDTHF
                     ELLGFLIPMPVFRPLVRRHFLRRIPWEVEKNLSRVAGQWADAVGASIDGLFRDGAEFL
                     EREVGTIAGLIGEAEAGDRLEEIYAVLAELSALESAVS"
     gene            complement(4491..4835)
                     /locus_tag="VF357_07100"
     CDS             complement(4491..4835)
                     /locus_tag="VF357_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX7523952.1"
                     /translation="MPKLVGENVLMRIFIGEGDKFGARPLHEALVELLKKERFHGATV
                     LRGVAGFGAHSVYHTDKILRLSRDLPMIVEVVDTEENVERVMPKLDEMIVGGMITLEK
                     VKVMRYTHPHAE"
     gene            complement(4861..5235)
                     /gene="crcB"
                     /locus_tag="VF357_07105"
     CDS             complement(4861..5235)
                     /gene="crcB"
                     /locus_tag="VF357_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX7523953.1"
                     /translation="MTVLLYITFFGAIGCLARYFLSGWVYTLLGTSMPYGTLAVNVIG
                     AFLIGLVMEFSLRTTQVSPDLRIGLTYGFLGGFTTFSSFSYETFRLLESAQLPQALAN
                     ILLSVTACLAFTFLGIVAARHL"
     regulatory      complement(5296..5364)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(5430..6308)
                     /locus_tag="VF357_07110"
     CDS             complement(5430..6308)
                     /locus_tag="VF357_07110"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7523954.1"
                     /translation="MMPEAPWEGALRRLEGIMARVEALLGKRDAPPTDAAIFQSHLAF
                     RWERAGEGGRIVPIPHPHRVDLASLVGIDGAKEELLRNTDQFVSGRGANHVLLWGERG
                     TGKSSCVKGLLPVFGPRGLRIVELARWDLFSFPKIVGQLRELPFRFLLYCDDLSFDEG
                     EADFRGLKTLLDGGVEERPENVLIYATSNRRHLMPEQRVALGAEDELHPEEAVSEKLS
                     LSDRFGLQLGFYRFDQETYLAVVESYARRLGLPVDPATLREEALRWALSAGSRSGRTA
                     KQFIEDLAGRLGTSAR"
     gene            complement(6305..7054)
                     /locus_tag="VF357_07115"
     CDS             complement(6305..7054)
                     /locus_tag="VF357_07115"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX7523955.1"
                     /translation="MIMKGRVAVVTGASRGIGAATAKRLARAGAAVAVNYFQSETAAG
                     EVVAAIREVGGTAIAVQADVRDASQCEAMADEAKRKLGPVDVLVLNASISFPVVPFLK
                     YPWPEFEAKLTGELKSAFFCCKAFVPGMVERRKGSVIAISSGLSRHPGEGFCAHSTAK
                     SGLDAFVKSLALELGPNGVRVNVVAPGLTLTDATSFLSQKEKDASAQMTPLRRNGLPE
                     DVAGAVLFLASEEARFITGAYLPVSGGIQMP"
     gene            complement(7153..7473)
                     /locus_tag="VF357_07120"
                     /pseudo
     CDS             complement(7153..7473)
                     /locus_tag="VF357_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
     gene            complement(8106..8393)
                     /locus_tag="VF357_07125"
     CDS             complement(8106..8393)
                     /locus_tag="VF357_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523956.1"
                     /translation="MRRTWIVLCTALLIAGLSAGCATSNAIQSARAQLQKAKGAGADW
                     SAPFEYYAGEAYLKKAVVEAEEGDTKAANVFTEQSRTYSAQALQKTGGGAK"
     gene            9015..10457
                     /locus_tag="VF357_07130"
     CDS             9015..10457
                     /locus_tag="VF357_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX7523957.1"
                     /translation="MKKLLLSLGILVALVVLGGVAILVLVDVNTYKPRIESAVSDALG
                     MEFRIGGKAHLRLFPSTSIVLSDVRLRNRGTDLATAETLRIGVKLRPLLAREVVITKL
                     ALEKPVIRIEKGVDGKFNYETPPRPATPATKEGEASSAPLTVSTGSASAGKIVYVDRK
                     AKSETSLDAIDLSVRDLSIPTAPGGKLWKGISFSGDLSVKEMKTKGFTVSDVRAKVTA
                     GAGVYEIRPFTMKLFGGTGEGGIHVDLSGEKTALKVKYTLAKFRAEDSLAGLAQKKYL
                     SGPMTVTPDLSLRGKDTDEMMRTLSGTVSLRGDGLTLHGMEIDPILSKAEDAQNLSLA
                     DVGAFVLAGPLGSAALKGYRYGDLYRSATREGETRIPRLVSDWSVRDGVADARDVAFS
                     TGKSRIALKGKLDLVNGRFLDVTVAALDEKGCAKIRQKISGPFGDPRMDKTSVLQSFA
                     EPILGLFERARKLVGRTSCEPFYTGDVQHP"
     gene            10611..>11075
                     /locus_tag="VF357_07135"
     CDS             10611..>11075
                     /locus_tag="VF357_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523958.1"
                     /translation="MANVYHEVISPPDSEWRDYIQRGCERGVQSRIIESWQRCGRVGL
                     NPYERNIPVPVESSQFKKRLQESLEIVELYQFYIRRFSGLLEQLGACSFVCDTDGFIL
                     SRAGYGKELNFFDDISLSEGSSCCEKVIGTNAPSLALIIREPVVVTADEHYTK"
BASE COUNT         1895 a         3764 c         3396 g         2020 t
ORIGIN      
        1 gaggacgacc tcggcgaccg cgtccttctt cagctccacg accacgcgca tcccgtcgcg
       61 gtcggactcg tcgcgcaggt ccgagatctc ctcgatctcc ttgttccgca ccagctccgc
      121 catccgctcg atgaggcggg acttgttcac ctggtacggg atctcggtga cgacgatcga
      181 ctcccggtcc cccttcttcg ccttctcgat gaaggcccgc gcgcggatct ggacgctgcc
      241 gcgccccgtg cggtaggcgt cgcgcacccc gtcgagcccg tagaggatcc cgccggtggg
      301 aaaatcgggc gcagggacgt gctccatcaa atcgtcgatc gtgatgtcgg ggttgccgat
      361 cagggcgagc agggcgccga tcacctcgcc gagattgtgc ggcgggatgg aggtcgccat
      421 cccgacggcg atccccgcgc tcccgttcac cagcaggttg ggaacgcggg acggaagaac
      481 gcgcggctcc tgcagcgagc cgtcgtagtt ggggagcgtc tcgacggttt ccttgtcgag
      541 atccgacagc agctcccccg ccaccttcgc catccggacc tcggtgtacc gcatcgccgc
      601 ggccgagtcg ccgtccaccg atccgaagtt tccctgcccg tcgatcaggg ggtaccgcag
      661 cgagaagtcc tgcaccatcc gcacgagggc gtcgtacacc gcgttgtcgc cgtgcggatg
      721 atatttgccg atcacgtcgc cgacgatgcg ggcggacttc ttgtacggct tgccgtactc
      781 gttgccgagc tcgtgcatgg cgaagaggat gcggcgctgg acgggcttga ggccgtcccg
      841 cacgtcgggg agggcgcgcc ccacgatcac gctcatcgcg tagtcgaggt agctctggcg
      901 catctcgtcc tggatggcgc ggggctgtgc ctgcttctgg aacaggtcca tgggggttcg
      961 ggtgctcctt cgaaggatcc ggatcgatgc ggcgggatcg ccgcctcacg acccgctgca
     1021 acggattatc ttaccacatc gaaggttgcc ggtgcgccga tgaaacctta aataataccg
     1081 ttagttatga tcattcccgc cgtgtcaccg tttgaggacc gcgccggtgc ttgcgctggt
     1141 ggccatcgcc gcgtattttc cgaggcagcc ggccgatttc tccttcggcg ggagctgcca
     1201 tttcgtgcgc cgttcggtga gttccgcatc ggagatgagc aggtcgagct tgcgcgccgg
     1261 gatgtccagc cggatccggt cccccttccg gaccaaggcg atcgtccccc ccgcggccgc
     1321 ctcgggggag acgtgaccga tgcaggcgcc gcgcgtccct ccggagaagc gcccgtcggt
     1381 gaccatcgcc accttgtcgc cgagcccctc gcccatgatg tacgacgtcg gcgcgagcat
     1441 ctcctgcatt cccgggccgc ctttgggccc ctcgtaccgg atgacgacga agtcgcccgc
     1501 cttcaccttc cccccgagga ttccctcgca cgcctcgtcc tgcgactcga agatcacggc
     1561 gggtccctcg aagaccagca tcttcgggtc gaccccggcg gtcttgacga ccgcgccttc
     1621 catcgcgagg ttgccgtaga ggaccgcgag ccctccttcc ttgctgtacg cgttggacag
     1681 ggtccggatg accgccgggt ccttcgatac cgcgatcgcc acgttctcct ccagggtctt
     1741 ccccgtcacg gtgaggcagc cccgtttcat gagacccggc accttgaaca gctccgaaag
     1801 gatcgccggg atcccccccg cgcggtccac gtcctccatg tggtactgcg acgacggcga
     1861 taccttgcac aacgtaggcg tcttccgcga gatcgcttcg atccgcgcca ggtcgaacga
     1921 gacccccgcc tcgtgcgcca ccgccagcgt gtggagaatc gtgttcgtgg agccgcccat
     1981 cgccatgtcc agcgcgaggg cgttgtcgaa ggcgtcccgc gtgacgatgt cgcgcggctt
     2041 cgtgtccttg cgcaccaggt ccatcagccg gaacgccgcc tcgcggtaat agtcgccgcg
     2101 ttgcttgtcg atcgccagca ccgacgcgct ccccgggagc gccatcccga gcgcctcgca
     2161 caggcaattc atcgagttcg ccgtgaacat gccggagcag catccgcagg tggggcaccc
     2221 ggtgtcctcg agctccttca gcccctcgtc cgagaggttc ccgagctggt gggccgccac
     2281 cccctcgaag acggagatca ggtcaacgac ccgaccggcc ttcgtcaggc cggccttcat
     2341 cggtccgccg gagacgaaga tcgtcgggat gttgcaccgc atcgccgcca tcaacatgcc
     2401 ggggacgatc ttgtcgcagt tggggatgca gatcatcccg tcgaagcagt gggccttgag
     2461 catcgtctcg acggagtccg cgatgatctc ccgcgacggc agggagtaga gcattccgcc
     2521 gtgtcccatg gcgatcccgt cgtccacgcc gatcgtgttg aactcgaagg cgacgccccc
     2581 cgcctcccgg acgcactcct tcacgtaccg cccgacccgg ttcaggtgca cgtggcccgg
     2641 gatgatctcc acgaacgagt tgcacaccgc gatgaacggc ttccccatgt cggcgtcggt
     2701 gacgccggtg gctttcagca ggctgcggtg tggcgcccgc tcgaacccct tcttgaccgt
     2761 gtcggaccgc atcggggacc tccggggatc ggctgttttg attttccagc gatcaggata
     2821 ccgcagattc gagggcggaa agctccgcga gtacggcata gatctcctcg agccgatccc
     2881 ccgcttcggc ttcgccgatc aaccccgcga tcgtcccgac ctcgcgctcg aggaactccg
     2941 ccccgtcccg gaaaagaccg tcgatcgagg cgcccacggc gtcggcccac tggcccgcca
     3001 cgcgggagag gttcttctcg acctcccacg ggatcctgcg caggaaatgc ctgcggacca
     3061 gcggccggaa gaccggcatc gggatcagga acccgagcag ctcgaagtgg gtgtcgaacg
     3121 tgggggagag ccggacatcg gggtgggccg gttcttcgac ctgcggatcg aaccgggccc
     3181 cttcgaaacg gatcccgagg gcgcgctcga tctcccccgc cagccggtcc gcgaaggccc
     3241 gcaccgaccg ctcgacgctc gcctgcgccc tgaagagaaa gcccgccagg tgcccctccc
     3301 catgcaacga gacggccccc atctcctcca tcatcgcgtc ggcgagccac ccctcgaact
     3361 cccgggtcac ccgtccgagg tgccccttcc accccgtcat cgcctcccgg agagcctcgg
     3421 ttacccgtcc cgtcacttca ccgtggtagg cgtgaaaccg ctcgtccgcc gcggtccgga
     3481 cctcggcctg gaggtggttg cagaggagtc ggagctcccc ctttacgggg ccgaagccgc
     3541 gccgctcgcg cgccagcgcc tcgatgagat cggaccgggc ggtgtcggcc gcctccgcag
     3601 cgcgttccgc cagattcagg tattcacggc agccggcgac cagcgcacgg accttgtgat
     3661 gcacgatctc tccgaacgcc tcctcccgcc gccccgcgat ccgttcccga aggtactccg
     3721 ccacttcctt gcgcatcccc tcgtaaccgg gacggttgga gacggggaga atcctccact
     3781 ccttccccgt tcgggaagtc gcctgactcc gggtgaattc gatcaccgtg gcgagctcct
     3841 ccccggacac caggtccgcc ttcgtcagca ggagggccgc ctcgggagaa tgcgaggaaa
     3901 cgtcgttgag gaggagcagg tcgtcttcgg aaagagggtg gttgacgctg acggcgacga
     3961 gggccgcccc gatgcgaggc atccattccc tggacacctt cgtgttgtgg gcgaagacgc
     4021 tgcccagccc cggggtatcg acgaaccgga tcccctcgta cgacgcgagg gcgggcagtt
     4081 ccacgtcgac caccgccacc cgcttttcgt tcgcggggtt tcccctctcc gtgacgaact
     4141 cggccagccg gcccagcggg atctcgaacg gttcccccgc aaggccgcac acggtgaccc
     4201 gctccctttc cccatacccg atccttgtga tcacggccgt cgacggcagg acgtccaccg
     4261 gcacgatcgt ctccccgatc agtccgttga ggaaggagct ctttcccgcc ttgaactgcc
     4321 cgagaacggc cacgtccacc acgccgccgc cccccaggag ctcctcacac gcggcgagct
     4381 gcggcgcgag ggagtcgatc cggaaacgac ggcagatcgc gccgacgcgg gcgacggttc
     4441 cggacaggtc catcccgggg aatcctccct gtgaagctag tgcagcgtcc ctattctgcg
     4501 tgcgggtgtg tgtaccgcat caccttgacc ttctcgaggg taatcatccc gccgacgatc
     4561 atctcgtcga gtttcggcat cacccgttcg acgttctcct cggtgtccac cacctccacg
     4621 atcatcggga gatcgcgcga cagccggagg atcttgtcgg tgtggtagac gctgtgggcg
     4681 ccgaaccccg ccacgccgcg cagcaccgtg gctccgtgaa accgttcctt cttgaggagc
     4741 tccaccagcg cctcgtgcaa gggacgggcg ccgaacttgt ccccctctcc gatgaagatc
     4801 cgcatcagga cgttctcccc gacgagtttc ggcatctttc tcctcctttc ggccctctgc
     4861 ttacaggtgg cgcgcggcga cgatcccgag aaacgtgaac gccaggcagg cggtgacgct
     4921 caagagaatg ttcgccagcg cctgcggcag ctgtgcgctc tccagcaggc ggaaggtctc
     4981 gtagctgaag gatgagaacg tggtgaaccc cccaagaaaa ccgtatgtca gaccgatacg
     5041 aaggtcgggg gatacctgcg tggtacggag actgaactcc atgacgaggc cgataaggaa
     5101 cgccccgatc acgttcaccg ccaaggtccc gtacggcatc gacgtaccga ggagggtata
     5161 gacccacccg gagaggaagt agcgcgcgag gcaaccgatg gcgccgaaga aggtaatgta
     5221 caggaggacg gtcattggtg cgaccctccg gaacgttgga tgccgagagg ataaaaaaaa
     5281 ctcctgcccc acatgtgccg ggtaggagtc atcagcctcg caaggtcgaa gcggttcacg
     5341 gcgaactcca tcgccttgtt gccaccatta caccacagcc gaccccggca ggaaagcgcg
     5401 gggcgtcgcg tatggtaatc cgcgggcgtt cagcgggcgg acgtcccgag gcggcccgcg
     5461 aggtcctcga tgaactgctt cgccgtccgg ccgctgcggg agccggcgga gagcgcccaa
     5521 cgcagcgctt cctcccgaag cgtcgccgga tcgacgggta ggcccaagcg ccgcgcgtat
     5581 gattcgacga cggcgagata cgtctcctgg tcgaaccggt agaaaccgag ctgcaggccg
     5641 aaccggtcgg agagggagag cttctccgag accgcctcct ccgggtggag ctcgtcctcc
     5701 gcgcccagcg cgacccgctg ttcgggcatc aggtgccgcc ggttcgaggt tgcgtagatc
     5761 agcacgttct ccgggcgctc ctccacgccg ccgtccagca aggtcttgag cccccggaag
     5821 tcggcctccc cctcgtcgaa cgacagatcg tcgcagtaga ggaggaaccg gaacggcagt
     5881 tcacggagct gcccgacgat cttcgggaag gaaaacaggt cccaccgggc gagctcgacg
     5941 atccgcaggc cccgcgggcc gaagacgggg agcagcccct tcacgcacga cgatttcccg
     6001 gtaccgcgct ccccccacag aagaacgtgg ttcgcccccc gcccggaaac gaactggtcc
     6061 gtgttccgga gcagctcctc cttcgccccg tcgattccga cgagcgaagc gagatccacc
     6121 cggtgcgggt gcgggatcgg gacgatccgg cccccctccc ccgcgcgctc ccaccggaac
     6181 gccaggtggg actgaaagat cgccgcgtcg gtcggcggcg cgtcccgctt cccgaggagc
     6241 gcctcgacgc gcgccatgat cccttcgagg cgccgaagcg cgccttccca gggcgcctcc
     6301 ggcatcatgg catctggatc ccgccggaaa ccggcaggta cgcgcccgtg atgaagcgcg
     6361 cctcctcgga ggcgaggaag aggaccgccc ccgccacgtc ctccgggagg ccgttccgcc
     6421 gcagcggcgt catctgcgcg gaggcgtcct tctccttctg cgaaagaaac gacgtcgcgt
     6481 ccgtcagggt gagccccggg gcgaccacgt tcacgcggac gccgttcggc cccagctcga
     6541 gcgcgagcga cttcacgaag gcgtcgagcc cggacttcgc cgtgctgtgc gcgcagaatc
     6601 cctctcccgg gtgtcgggac agcccgctgc tgatcgcgat gaccgacccc ttgcggcgct
     6661 ccaccatccc cggcacgaac gccttgcagc agaagaaagc ggacttgagc tcccccgtca
     6721 gcttcgcctc gaactcgggc cacgggtatt taaggaaagg tacgacgggg aaggagatcg
     6781 acgcgttcag cacgagcacg tcgaccggcc cgagcttccg tttcgcctcg tccgccatcg
     6841 cctcgcactg cgacgcgtcg cggacgtccg cctggacggc gatcgccgtc ccgccgacct
     6901 cccggatcgc cgcgaccact tcccccgcgg ccgtctcgct ctggaaatag ttgaccgcca
     6961 ccgcggcgcc ggcccgcgcg aggcgcttcg ccgtcgccgc cccgatcccg cggctggccc
     7021 cggtgaccac cgccactctc cccttcatga tcatcgcgtc tccccccttc tctccgaaaa
     7081 aaaacccggc acggcattcc ccggaacctc ggagaatgcc gtgccggaaa gattccttcc
     7141 gacaaaccct ctctattcgc cgatgatcag aatttccact ctccggttct cgaaccggcc
     7201 tttatccgtc ttgttgtccg cgattggttc ggatttccct tttgcctccg tcgtgatccg
     7261 atcggccttc tcccccttgt ccacgaggta ctttttcacc gcatccgctc tccgctggga
     7321 aagcttcagg ttatatttaa cagtcccgcg gctatccgtg tggcctacca ggacaacttt
     7381 ggagtccggg tacttcttca agaaagcggt cgctttctcc agttccgcga catccgattt
     7441 ccggattttg gacttgtcga agtcgaagtt gacgtgaatg gtcaggcgat cgatcaccac
     7501 ccgggccggg gccggtcccg gggccggtac cggggccgcg gtcggaggag gaggcggcgc
     7561 cggagccgtc accgtgacgg ttgccgtctc gtaagcggtc ccaccggtcc cggtcgcggt
     7621 caacatgtac tgggtggttt cggcggggca cacttccttg gagccgctcg cagcgacgtt
     7681 tcctacgccc tgatcaatct ccgccgtttt cacgttttcc gaagtccact tcagggtcga
     7741 gcactttccg ggggcaacct ccgccgggtc cgccgaaaaa ttcacaacag gaggttgaca
     7801 cgccttcgtc ttggcaagaa ccgtatcggc tgccttgccc gctccggtga tgaactgtgg
     7861 cgcggtttcc cagggctcgg tcgtctcgtg aaacgcataa tccttttccg acttggcggc
     7921 agccagttcc ttgggcgcgc actccttggc gcccttgggg gtcatatttt ccgcctggtc
     7981 gatcttgccc gagatgacat tgaactggtc cgtttcaccc ttggtcagct ggcgggtggc
     8041 gccgcagccg gacaccagga acgccgcgac aagcaacgac aggagaagga taagattccg
     8101 gttcctcatt tcgcacctcc cccggtcttc tggagcgcct gggcggaata cgtccgcgat
     8161 tgctccgtga agacgttggc ggccttggtg tccccttctt ccgcctcgac gaccgccttc
     8221 ttgaggtacg cttcccctgc gtagtattcg aaaggcgccg accagtcggc tccagctcct
     8281 ttcgctttct gcaactgcgc ccgggcgctc tggatcgcgt tggacgtcgc gcaaccggcg
     8341 gacagcccgg cgatcagtaa cgccgtgcat aggacaatcc atgttcgcct catcgaaacc
     8401 ctccttttca tgtgttgtct tcaccgaaga accttttcac cacgtcaaac cctacgttcc
     8461 gaacaccctc ctcccgcgga tttcgccacc acctttcagg cagtcggccg tttctatacc
     8521 cgaattatac aatcatacat cgatactatt aaatatcatt aaacggattt tcctggcgca
     8581 atccacgcga tagaccgtgg ccgctcgcat cgttttctcc tgttttcatc gtccttcaac
     8641 cagtagaaac ccacattccc tgatgtctca cacgataatc ttgcccgcca gcgaacggta
     8701 tcggtttctg tccgaaacgc ggatcggtgt ttgtcctatg gtttcaaccg aagttccccg
     8761 ccagtttcat gggccttgct tcctggtatt cccctgataa cgggcagttg ttcttttttc
     8821 ctcgttcatg tttcgtgcag aatgcagaca tgtatatcat aaattttccc gcacggaagg
     8881 atcccggggt gccgttgctc gacatagatt gaaataaatg catggtcgaa tcccggtccg
     8941 tcttgccgtt tgaccggaag gacgggccgg cgcaagatgg ttcctgtcac ctgacgcggg
     9001 aggatcatca ccccatgaag aagctcctcc tctccctcgg catcctggtc gccctcgtcg
     9061 ttctcggggg ggtcgcgatc ctcgtcctcg tcgatgtgaa cacctacaaa ccccggatcg
     9121 aatccgccgt ctccgatgcg ctcggcatgg agttccggat cggcgggaag gcgcacctgc
     9181 ggctctttcc atcgacgagc atcgtcctct ccgacgtgcg gctgcgcaac cgcggcaccg
     9241 accttgccac ggcggaaacg ctgcggatag gggtgaagct gcgtccgctt ctggcccgcg
     9301 aggtcgtgat cacgaaactg gcccttgaaa aaccggtgat ccggatcgag aaaggggtcg
     9361 acgggaagtt caactacgaa accccgccgc gcccggcgac gcccgcgacg aaggaaggcg
     9421 aagcctcttc cgcacccctg accgtttcca ccggttccgc gtcggcgggg aagatcgtgt
     9481 acgtcgaccg gaaggcgaag agcgaaacga gcctcgacgc gatcgacctg tccgtgcgcg
     9541 acctctcgat ccccacagcc ccggggggga agctctggaa ggggatctcc ttctccggcg
     9601 acctgtccgt gaaggagatg aaaacgaagg gcttcaccgt gtcggacgtg cgcgcgaagg
     9661 tgacggccgg cgcgggggtc tacgagatcc gtcccttcac gatgaagctc ttcggcggca
     9721 cgggcgaggg ggggatccac gtggacctct ccggggaaaa gacggccctg aaggtgaagt
     9781 acacgctggc gaagttccgc gccgaggatt cgctcgcggg gctcgcgcag aaaaaatatt
     9841 tgagcgggcc gatgacggtg acaccggacc tctccctccg ggggaaggac acggacgaga
     9901 tgatgcggac gctctcgggc accgtctccc tgcgaggaga cgggctgacg cttcacggga
     9961 tggagatcga cccgatcctg tcgaaggcgg aggatgcgca gaacctgagc ctggcggatg
    10021 tgggcgcgtt cgtgctcgcg ggcccgttgg gatccgcggc gttgaagggg taccgctacg
    10081 gggacctcta ccggtccgcc acccgggagg gggaaacccg gatcccccgt ctcgtctccg
    10141 actggtcggt gcgcgacggc gtggccgacg ccagggatgt cgccttctcc acggggaaga
    10201 gccggatcgc gctgaagggg aaactcgacc tcgtgaacgg gcggttcctg gacgtcaccg
    10261 tcgcggcact cgacgagaag gggtgcgcga agatccggca gaagatctcg ggtcccttcg
    10321 gcgatccgcg gatggacaag accagcgtgc tgcagtcgtt cgcggagccg attcttgggc
    10381 ttttcgagcg ggcaaggaaa ctggtcgggc ggacatcgtg cgagccattc tacacggggg
    10441 acgtgcagca cccttagtgc tccaatccat gaatacggta gcatccgagt gccgctgcgc
    10501 ctgtttccgg cgagcgagga aatcggcagg gatcgggacc ccgcttgacg gtatgccata
    10561 gtctggtaga atatcaaaca cctttttaaa cccaggaggg aaggaacttc atggcgaacg
    10621 tctatcacga ggttatttct cctcccgatt ccgagtggcg ggattatatc cagcggggat
    10681 gcgagcgggg ggtccaaagc aggatcatcg aatcgtggca gcgttgcggg cgggtgggcc
    10741 tcaacccata cgaacggaac atccctgtgc cggtcgagtc ctcccagttc aaaaaacggt
    10801 tacaggagag cctcgagatc gtcgagttgt atcagttcta catacgccgc ttttccggat
    10861 tgctggaaca gctgggcgcc tgttccttcg tctgcgatac cgacgggttt atcctctcgc
    10921 gggcgggata cggcaaggag ctgaacttct tcgacgatat ctcgctctcc gagggcagtt
    10981 cctgctgcga aaaagtgatc gggacgaacg ccccgagcct ggccctcatc atccgcgaac
    11041 cggtggtggt cacggccgac gaacattaca cgaag
//
LOCUS       DASVJL010000338         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1088182, whole genome shotgun sequence.
ACCESSION   DASVJL010000338 DASVJL010000000
VERSION     DASVJL010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088182"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VF357_07140"
     CDS             complement(<1..486)
                     /locus_tag="VF357_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7523959.1"
                     /translation="MSIRSPRRIVCYPHRGIICPLSPAHIAGFAAFHLYVILLFVGIR
                     TAPLPLLAFLLACAVAPFLPRIGFFLPIVGRGKPGAKGVALTFDDGPDPEVTPLLLDL
                     LDLHSVSATFFVTGERAARHPSIIRDILSRGHAIGNHSYHHFPFLMLKGIRTLRREIE
                     ST"
     gene            complement(491..1336)
                     /locus_tag="VF357_07145"
     CDS             complement(491..1336)
                     /locus_tag="VF357_07145"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEX7523960.1"
                     /translation="MIKTHPRIAGMILCAALFLGLSQPAAGERVPQWELGAGVAGLML
                     PDYRGSDEVRSYLLPVPYIIYRLEWLKADQTGIHSILLNWEAAEVNLSLGATPPVRSK
                     KNRAREGMSDLKPMVEFGPSLDIHLWRSDDRRFKLDVRTPVRAAFTVESHPRDAGMSL
                     SPTLNFDVAGIGGRPWQLGMLAGPIFATRRQHQYFYGVPESDARPDRPAFDARGGYAG
                     LQFLVALSRRFEKAWFGAYARYDTLRGAVFEDSPLVRRPFYVSAGFAIAWIPFQSSRM
                     IERDD"
     gene            complement(1333..2445)
                     /locus_tag="VF357_07150"
     CDS             complement(1333..2445)
                     /locus_tag="VF357_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX7523961.1"
                     /translation="MSGTGRIVIEGVGVVGGFGCGIHDLSRALTGNARSPGTLALSTG
                     SGPVTIPAFRADVSRLEEFLPVRTLRRIDHFSRLGLLGSHLALADAEMPEAERRQLGI
                     IIASGYGAAGITYAFLKSFISDGDIGASPTYFSSSVHNSAAAHISIQLGAKGPNLTVS
                     EFHLSVPSALATARIWLAEERVQRVLFGAVDELSELIGYIRYRTRDASPTASMTPLRT
                     EVETSIPAEGAAFLLLARENTSRGGYCTLDASTTGSLPIPGLPFPEPGLLVLGADGCR
                     ESGRRYAEVAQNTRVACYTPLYGSMPAGPAFDLAAAALILKGGRVFPSPGGVSRDFPG
                     TVPVGGEPVGADRISCLTLAGDETYGLTTLGRVDSQ"
     gene            complement(2442..>2795)
                     /locus_tag="VF357_07155"
     CDS             complement(2442..>2795)
                     /locus_tag="VF357_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523962.1"
                     /translation="SGVSSTSLAFVNAHGTGTPDNDRVESRVLHDMLPGIPFLSTKGY
                     TGHTLGAAGAIEAVFTAACLEMGKIPANAGFETPDPEIGIAPVREVTAVSGTVALSES
                     LAFGGNNSVVVLGKA"
BASE COUNT          507 a          873 c          989 g          426 t
ORIGIN      
        1 ggtcgattcg atctcccgcc ggagcgtccg gatacctttc agcatcagga acggaaagtg
       61 gtggtaggag tggttgccga tcgcgtgacc gcgggacagg atgtcccgga tgatggatgg
      121 atggcgggcc gcccgctccc ccgtcacgaa gaacgtcgcc gaaaccgagt gcaggtcgag
      181 aaggtccagg aggagggggg tgacctccgg gtccggcccg tcgtcgaacg tgagggccac
      241 gcccttcgcc cccggtttcc ccctgccgac gatcgggagg aagaagccga tgcggggcag
      301 gaacggagcg acggcgcagg cgaggaggaa ggcgagcaac gggagcggcg ccgtgcggat
      361 gccgacgaac aggaggatca cgtacagatg gaacgcggcg aaccccgcga tatgggccgg
      421 cgacaggggg caaatgatcc cccggtgcgg gtagcaaacg attctcctcg gggatcggat
      481 ggacaccggg ctagtcgtcg cgctcgatca tcctggaaga ttgaaacggt atccacgcga
      541 tcgcgaagcc cgcggaaacg tagaagggac ggcgtaccag aggactgtcc tcgaacacgg
      601 cgccgcgcag cgtgtcgtaa cgggcatagg cgccgaacca cgccttctcg aatcgccgcg
      661 acaacgcgac aaggaactgc aggcccgcgt agccgccgcg cgcatcgaat gccgggcgat
      721 ccggacgcgc gtcgctttcc ggcacgccgt agaaatattg atgctgacgg cgcgttgcga
      781 agatcggccc ggccagcata ccgagttgcc acggccggcc gccgatgccg gcgacatcga
      841 aattcagggt gggcgagaga ctcatgcctg cgtctcgcgg atgcgactcg acggtgaacg
      901 ccgcccggac cggcgtgcgc acgtcgagct tgaagcgacg atcgtcgctg cgccacaggt
      961 ggatgtccag cgatggaccg aattcaacca tcggcttgag atcgctcatg ccttcgcgcg
     1021 cgcgattttt cttgctgcgc accggcggcg ttgcgcccag gctgaggttg acctcggccg
     1081 cctcccagtt gagcaggatc gaatgaatgc cggtctggtc ggccttgagc cactccaggc
     1141 ggtaaataat gtagggaacg ggcagaaggt agctgcggac ctcgtccgag ccgcggtagt
     1201 cgggcagcat caaaccggca acgcctgcgc ccagctccca ctgcggcacc ctctcgccgg
     1261 cggcgggttg cgacaacccc aggaagagcg ccgcgcaaag gatcatgccg gcgatgcgag
     1321 gatgggtctt gatcattgcg aatccaccct tccgagcgtc gtcaatccgt aggtttcgtc
     1381 tccggcgagc gtgaggcagg agatccgatc ggcgccgacc ggttcgccgc cgacgggaac
     1441 cgttccgggg aagtcgcggg agacgccgcc cggggaaggg aatacgcgcc ccccctttag
     1501 gatgagcgcg gccgccgcca ggtcgaaggc cggccctgcc ggcatggacc cgtacagggg
     1561 tgtgtagcat gcgacgcggg tgttttgcgc cacctccgcg tatcgtcggc ccgattcgcg
     1621 gcagccgtcc gcccccagaa ccagcaagcc cggttccgga aagggaaggc cgggaatcgg
     1681 caggcttccc gtggtcgatg cgtccagcgt gcaatacccg ccccgggacg tgttctcccg
     1741 ggcgaggagc aggaaggccg ctccttccgc ggggatcgac gtctccactt ccgtccgcag
     1801 cggggtcatg gaagcggtcg gcgacgcgtc ccgcgtccgg taccggatgt agccgatcag
     1861 ttcggacagc tcgtcgaccg cgccgaacag gacccgctgg accctttctt ccgcaagcca
     1921 aatccgggcg gtggcgagtg cggagggaac cgacaggtga aattcgctga cggtgaggtt
     1981 cggccccttg gcgcccagct ggatcgagat gtgggcggcc gcggagttgt ggaccgagct
     2041 ggagaaatag gtcggcgatg cgccgatgtc gccgtcgctg atgaacgact tgaggaatgc
     2101 gtacgtgatt cccgccgcgc cgtagccgct ggcgatgatg atgcccagtt ggcgtctttc
     2161 cgcttcgggc atttccgcgt ccgcgagcgc aaggtggctc ccgaggagac cgagccgcga
     2221 aaaatggtcg atccgccgca atgtgcgaac ggggaggaac tcctcgaggc gggacacatc
     2281 ggcccggaag gcggggatcg tgaccggtcc ggatcccgtc gacaacgcga gggtccccgg
     2341 ggaacgggcg tttccggtca acgcgcgtga caggtcgtgg atgccgcagc cgaagccgcc
     2401 gaccactccg accccctcga tgacgatccg gcccgttccg ctcatgcctt gccgaggacg
     2461 acgaccgagt tgttcccccc gaatgcgagc gattcggaga gggcgacggt tcccgacacc
     2521 gcggtgactt cacgaacggg ggcgatcccg atctcggggt cgggggtttc gaacccggca
     2581 ttggccggga ttttccccat ctcaaggcag gcggcggtga acaccgcctc gatcgcgcct
     2641 gcggccccga gggtatgccc ggtgtaccct ttcgtggaga ggaaagggat gccgggcagc
     2701 atgtcgtgga ggacacgact ctcgacccgg tcgttgtcgg gcgtgccggt accgtgggcg
     2761 ttgacgaacg ccaaggaagt ggaggaaacg ccgct
//
LOCUS       DASVJL010000339         1717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_900918, whole genome shotgun sequence.
ACCESSION   DASVJL010000339 DASVJL010000000
VERSION     DASVJL010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900918"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VF357_07160"
     CDS             complement(<1..485)
                     /locus_tag="VF357_07160"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX7523963.1"
                     /translation="MAFSGTKSCGDGPTKDPAEGKRPIPGVRNVVAVASGKGGVGKST
                     ISANLAVALARTGARVGLLDADIYGPSVPTLFHLKGHRLAGEDGMIVPAEAQGLKIVS
                     IGFLLEDDSPVIWRGPMLMKALEQFLHGTKWGELDYLVIDLPPGTGDVQISLVQMTPV
                     AG"
     gene            663..>1717
                     /gene="murJ"
                     /locus_tag="VF357_07165"
     CDS             663..>1717
                     /gene="murJ"
                     /locus_tag="VF357_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX7523964.1"
                     /translation="MTDRPGRQMGRAAALMMGSVLLSRILGYARDAVIAWQHGATSET
                     DAYFVAFTIPDFLNYLLAGGALSITFIPIFARYLAEGREEEGFRSFSTIATVMGIGML
                     FFIVLGEFLAGRVIPLLAPGFSPAQVALAARLTRIVLPAQIFFYLGGLLMAVQYARKQ
                     FFLPALAPLIYNAGIIAGGLLLGRDHGMEGFAWGVLVGAFLGNFALQIHGARRGGLSF
                     FPRLDFSDPGLKEFLRLSIPIMLGFSLVVVDEWTTRVFGSFLLAGAITWLNNARRLML
                     VPVGIFGQAAGVASYPFLSSLAAEGKKAEMWNTLSLTLRWVFLVSAGTAAIAVILSRE
                     AVLLVYQRGAFTIDDTMR"
BASE COUNT          233 a          621 c          543 g          320 t
ORIGIN      
        1 ccggccaccg gcgtcatctg gacgagggag atctggacgt ccccggtccc cggcggcagg
       61 tcgatgacga ggtagtcgag ctccccccac ttcgtcccgt ggaggaactg ctccagcgcc
      121 ttcatcagca tcgggccgcg ccagatgacc gggctgtcgt cctcgaggag gaacccgatc
      181 gagacgatct tcagtccctg cgcctccgcc ggcacgatca tcccgtcctc ccccgcgagc
      241 ctgtgcccct taagatgaaa caaggtcggc acggagggac cgtagatgtc ggcgtcgagc
      301 agtccgacgc gggccccggt ccgcgccagc gctaccgcga ggttcgcgct gatcgtcgac
      361 ttcccgacac cccccttccc cgacgcgacg gcgacgacgt tgcgcacgcc cgggatcggc
      421 cgcttcccct ccgccgggtc cttcgtcggc ccgtcgccgc aagacttcgt tccggaaaac
      481 gccatatcct tcacctcacg cgttgatgca ctggatgata gcagggcgga cgcccgatgc
      541 ccatggacgg gcaagtgccg cgggagccat ggatggcggg agcggccccg ccgcgccctg
      601 cgtcccgaac ttggtacgtt accgtattta tgaaacgcag cacttagatt atagtttcaa
      661 ggatgaccga ccgcccgggc cgacagatgg ggcgcgccgc ggcgctgatg atggggtcgg
      721 tgctcctttc ccggatcctc gggtacgccc gcgacgccgt gatcgcctgg cagcacggcg
      781 ccacctccga gaccgacgcc tacttcgtcg ctttcacgat ccccgacttc ctcaactacc
      841 tgctcgcggg cggagcgctc tccatcacct tcatcccgat cttcgcgcgg tacctggcgg
      901 aggggaggga ggaggaaggg ttccgttcct tctcgacgat cgccacggtg atggggatcg
      961 gcatgctctt cttcatcgtg ctgggcgagt tcctcgcggg ccgcgtcatc cccctcctcg
     1021 cccccggctt ctctcccgcg caggtggcgc tcgcggcgcg cctgacgcgc atcgtgcttc
     1081 ccgcgcagat cttcttctac ctcggggggc tcctgatggc ggtgcagtac gcccggaagc
     1141 aattcttcct ccccgcgctg gcccccctga tctacaacgc cgggatcatc gcgggagggc
     1201 tgctcctcgg acgcgaccac gggatggagg ggttcgcctg gggagttctc gtcggggcgt
     1261 tcctcgggaa cttcgccctg cagatccacg gcgcgcgtcg cggcggcctc tcctttttcc
     1321 cccggctcga tttctccgac ccgggcctga aggaattcct ccgcctttcc atcccgatca
     1381 tgctcggctt ctccctcgtc gtggtggacg agtggaccac gcgcgtcttc ggctccttcc
     1441 tcctcgcggg ggcgatcacc tggctgaaca acgcgcgccg cctgatgctg gtgcccgtcg
     1501 gcatcttcgg ccaggcggcg ggggtggcgt cgtacccgtt cctctcctcg ctggcggcgg
     1561 aggggaagaa ggcggagatg tggaacacgc tgtccctcac cctgcggtgg gtcttcctcg
     1621 tctccgccgg gacggcggcc atcgccgtca tcctgtcgcg ggaggcggtc ctcctcgtct
     1681 accagcgcgg ggcgttcacg atcgacgaca cgatgcg
//
LOCUS       DASVJL010000340         3645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1368570, whole genome shotgun sequence.
ACCESSION   DASVJL010000340 DASVJL010000000
VERSION     DASVJL010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368570"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VF357_07170"
     CDS             complement(<1..406)
                     /locus_tag="VF357_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523965.1"
                     /translation="MSVLSVLASALRGGRRHPGSVKEVSLAAALTSGLYEVSQAMSAP
                     GGDVQHSLDLITSAAAAILRVERCVLLLPEPGVEHLVVRSMAGIPRARQFEKYRQEMY
                     KLVVHPILSSGTGMIVSEGRPGTDRVLLRLMRR"
     gene            complement(403..759)
                     /locus_tag="VF357_07175"
     CDS             complement(403..759)
                     /locus_tag="VF357_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7523966.1"
                     /translation="MMKVLVVDDEESIRELYRADLSDEGYEVGLAMDGRQALVLLESF
                     LPNLVTLDIKLPDIDGIEVLRRIREKNATIPVILLTAFGEFRQDFNTWASDAYIVKSH
                     DTTELKDTVRKLLGAG"
     gene            complement(764..1372)
                     /locus_tag="VF357_07180"
     CDS             complement(764..1372)
                     /locus_tag="VF357_07180"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HEX7523967.1"
                     /translation="MKVLLGISGASGAVYGIRTLSLLLARGAELHVLVTKTAWGILDA
                     EVGDGKLPGGADARRKWLARLSSSARPFRLYAEDEFGIPFASGSNAPDAMIVAPCSMG
                     TLGRIAHGVSSSVLTRCADVALKERKPLVLVARETPLSVIHLENMLTLARAGAYVLPA
                     CPGFYHRPETVSGLVDFVVSRALSCIGVDAGVLPRWGEGKEE"
     gene            complement(1369..2274)
                     /locus_tag="VF357_07185"
     CDS             complement(1369..2274)
                     /locus_tag="VF357_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEX7523968.1"
                     /translation="MEGVRILSVLRRIGVYLEMVKAAHTVFALPFALTGMFLAARELG
                     ARNALPPARTVFYIVLAMVGARSASMGFNRLVDAEIDAKNPRTRDRAIPSGQVSRPMA
                     RVFILMSVALLVLSAAKLNALCLQLTPVLLLLLFSYSYMKRFTWASHLILGACLGAAP
                     LAAWIAVTGAADARVLWICLAVLFWVGGFDVLYALQDIDVDRREGLHSIPRSLGVGPS
                     LWVARSFHVAMAGFLLLGYHVFGLGGWYLAGLALCAAVLGYEHAIVRKDDLSRLNMAF
                     FNLNGVVSIVFCLFTYLDLVLRGRP"
     gene            complement(2253..>3645)
                     /locus_tag="VF357_07190"
     CDS             complement(2253..>3645)
                     /locus_tag="VF357_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEX7523969.1"
                     /translation="ELKRIPSEVSPELEAAEIADRAVKAGGPAILFENVRGTSVPLAM
                     NLFGTMERMCFALGVPRLDDIAERVQEVIEPEIPTNFLEKLKMLPKLARLADFVPKTV
                     SSAPCQEVVEKDHPSLSFLPVVKTWPGDGGPFITLPLVFTKDPETGRRNVGMYRMHVY
                     DETTTGMHWHVHKGGAQHYRGFRGKRERMPVAVALGGDPATIYAASAPLPEDMDEMMF
                     SGFLRKEPVELVHCRTIDIEVPAHAEVILEGYVDPDEQRVEGPFGDHTGYYSLADRYP
                     VFHVTCVTRRKDPIYPATIVGKPPMEDVYLGKATERIFLPLLRKIVPEVTDMNLPIEG
                     IFHNFAFFSIDKRYPGHARKVMCAVWGLGLLMFSKFVVIFDSDVNIHDMREALWRIGN
                     NVDPRRDTMIVDGPVDALEHASPIPHYGSKMGIDATRKWASEGFAREWPADIAMPREI
                     IDLVTRRWKEYGF"
BASE COUNT          652 a         1254 c         1184 g          555 t
ORIGIN      
        1 gacggcgcat cagtcgcagg aggacacggt ccgtccccgg cctcccctcc gagacgatca
       61 tccccgtccc ggaggagagg atcggatgga cgaccaactt gtacatctcc tgccggtact
      121 tctcgaactg ccgcgcccgt gggatccccg ccatcgaccg gaccaccagg tgctccaccc
      181 cgggctccgg aaggaggaga acgcaccgct cgacccgaag gatcgccgcg gcggcggagg
      241 tgatgaggtc gaggctgtgt tggacgtccc cccccggcgc gctcatcgcc tgcgacacct
      301 cgtacaggcc ggaggtcagc gcggcggcca gcgacacctc cttcacgctg ccgggatgcc
      361 ttcttccccc ccgcagggcg gaggcgagga cggacaggac gctcatcccg cccccagcag
      421 cttgcggacg gtatctttca actccgtcgt gtcgtgggac ttcacgatgt aggcgtcgga
      481 agcccaggtg ttgaagtcct gacggaactc gccgaaagcg gtgagcagga tgaccgggat
      541 cgtcgcgttc ttctcccgga tccggcggag gacctcgatc ccgtcgatgt ccggcagctt
      601 gatgtcgagc gtcacgaggt tcggcaggaa ggattccaga aggacgagcg cctgccgccc
      661 gtccatggcc agccccacct cgtacccctc gtcggagagg tccgcccgat acagctcgcg
      721 gatgctctcc tcgtcgtcca ccacaaggac cttcatcatc gtctcactcc tcctttccct
      781 ccccccaccg cgggaggacc ccggcgtcga caccgatgca ggagagcgcc cgggacacga
      841 cgaaatcgac aaggccggag accgtctccg ggcgatggta gaaccccgga caagcgggaa
      901 ggacgtacgc ccccgcgcgg gccagcgtca acatgttctc gagatggatg acggacagcg
      961 gcgtctcccg ggccacgaga accaacggct tgcgctcctt gagggccacg tcggcgcacc
     1021 gggtcagcac cgacgacgac accccgtggg cgatccgccc gagggtcccc atggagcagg
     1081 gggcgacgat catcgcatcg ggcgcgttcg acccggaggc gaacgggatc ccgaattcgt
     1141 cctcggcgta caggcggaac ggccgggcgg aggaggagag gcgcgcgagc cacttccgcc
     1201 gcgcgtccgc gcctccgggg agcttgccgt ccccgacctc cgcgtcgagg atcccccacg
     1261 ccgtcttcgt gaccagaacg tgcagctccg caccgcgcgc aaggagcagg gagagggtgc
     1321 ggatgccgta caccgcgccg ctggcgcccg aaatgccgag aagaaccttc atgggcggcc
     1381 cctcaacacc aggtcgaggt acgtgaacag gcagaacacg atgctcacga ccccgttcag
     1441 gttgaagaac gccatattca gccgcgagag gtcgtccttc cggacgatcg cgtgctcata
     1501 ccccagcacg gccgcgcaca gggcgagccc cgcgagatac cacccgccca gtccgaacac
     1561 atgatacccc agaaggagga agcccgccat cgccacgtgg aacgaccggg cgacccacag
     1621 cgaggggcct actccgaggg accgcgggat cgagtggagt ccctcccgcc ggtcgacgtc
     1681 gatgtcctgc agcgcataga ggacgtcgaa cccgcccacc cagaacagga cggcgaggca
     1741 gatccagagc acccgggcgt ccgccgcgcc cgtcacggcg atccacgccg ccagcggcgc
     1801 cgcccccagg cacgcgccga ggatgaggtg cgacgcccac gtgaaccgct tcatgtacga
     1861 gtaggagaag agcagcagga gcaggaccgg ggtgagctgg aggcagagtg cgttgagctt
     1921 cgcggcggaa aggacgagca gcgcgacgga catcaggatg aagacgcgcg ccatcggccg
     1981 gctgacctgc cccgagggga ttgcgcggtc cctcgtccgg gggttcttcg cgtcgatctc
     2041 cgcgtccacc agccggttga atcccatgga ggcgctgcgt gcccccacca tcgcaaggac
     2101 gatgtagaag acggtccggg ccggggggag tgcgttccgc gcgccaagct cccgggccgc
     2161 gaggaacatt cccgtcaggg caaagggcag cgcaaagacg gtgtgcgccg ccttgaccat
     2221 ctcgagatag acgccgatgc ggcggaggac gctcagaatc cgtactcctt ccaccgtcgg
     2281 gtgacgaggt caatgatctc ccggggcatc gcgatgtccg cgggccattc gcgggcgaac
     2341 ccttccgaag cccatttcct cgtcgcgtcg atccccatct tcgagccgta gtgcgggatc
     2401 ggggaggcgt gctcgagggc gtccacgggg ccgtccacga tcatcgtgtc ccggcgcggg
     2461 tcgacgttgt tcccgatccg ccagagcgct tccctcatgt cgtggatgtt cacgtcggag
     2521 tcgaagatca cgacgaactt ggagaacatg agcagaccca gcccccacac ggcgcacatt
     2581 accttccgcg cgtggccggg gtaccgcttg tcgatggaga agaaggcgaa gttgtggaag
     2641 atcccctcga tcgggaggtt catgtccgtc acctcgggta cgatcttccg caggagcggg
     2701 agaaagatcc gctccgtcgc ctttccgagg tagacatcct ccatcggcgg cttcccgacg
     2761 atcgtcgccg ggtagatggg atctttcctg cgggtgacgc acgtcacgtg gaagacgggg
     2821 taccggtcgg cgagggagta gtaccccgtg tggtcgccga aggggccctc gacccgctgc
     2881 tcgtcggggt cgacgtaacc ttcgaggatc acctcggcgt gcgcaggtac ctcgatgtcg
     2941 atcgtcctgc agtgaacgag ttccaccggc tccttcctca gaaaaccgga gaacatcatc
     3001 tcgtccatgt cctcgggcag cggcgcggag gctgcgtaga tcgtcgccgg atcgccgccg
     3061 agggcgaccg ccacgggcat ccgttccctc ttcccccgga atccccggta atgctgcgcc
     3121 ccccccttgt gcacgtgcca gtgcattccc gtcgtcgttt cgtcatagac gtgcatccgg
     3181 tacatcccga cgttccggcg tcccgtctcc gggtccttcg tgaagaccag cggaagcgtg
     3241 atgaacggcc cgccgtcgcc gggccatgtc ttcaccaccg gaaggaacga cagcgagggg
     3301 tggtccttct cgacgacctc ctggcacggg gcggaggaga ccgtcttcgg tacgaagtcg
     3361 gcgagtcgcg cgagcttcgg gagcatcttg agcttctcga ggaagttcgt gggaatctcc
     3421 ggttcgatca cctcctgaac gcgctccgcg atgtcgtcga gccgcggtac gcccagcgcg
     3481 aagcacatcc gctccatcgt gccaaagagg ttcatcgcca acggcaccga cgttccccgg
     3541 acgttctcga agaggatcgc cggcccaccg gccttcaccg cccgatcggc gatctccgcc
     3601 gcctcgagct ccggggagac ctcggacggg atgcgcttca actcg
//
LOCUS       DASVJL010000341         8045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_337257, whole genome shotgun sequence.
ACCESSION   DASVJL010000341 DASVJL010000000
VERSION     DASVJL010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337257"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VF357_07195"
     CDS             complement(<1..984)
                     /locus_tag="VF357_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523970.1"
                     /translation="MSGWKKITLGIAGAFGLLAVLLGALTVLMPRWINGEAVKAGILA
                     RVSRAAGGTVRYERLDLVWFPRPRIVVRRLELAIPHRAAGTVESLTLSPSLFPLVRGG
                     YHLAKIRADAPDLAVEIPRSAKEEKPPSLAEIREQIAALLSTLSVDAPGMTFEVHRGR
                     VALSLGPRPLCALREIEGRIILPPKRLDVDLRCASNLWGNLSLNGRLEAAELSGRGTI
                     ELVGLDLAPFAESFLPEGWRQMAAGRADLGAGFEIERFRTLKAEVRGESRSLSVRRGN
                     GSTGVSGLRIGGTVEIDEARTVVSVARMSLESPRLRTSGQLVLDRTVQRATL"
     gene            1161..2171
                     /gene="gnd"
                     /locus_tag="VF357_07200"
     CDS             1161..2171
                     /gene="gnd"
                     /locus_tag="VF357_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX7523971.1"
                     /translation="MELGMIGLGRMGANMVRRLMRAGHRCIVYDTSAEAVAALAKEGA
                     TGADSLDDFLSRLSKPRIVWMMVPAAVVDRAIADLAPRLARGDVLVDGGNSYYVDDIR
                     RAKDLSAKGIHYVDVGTSGGVFGLERGYCQMIGGEPEIVKRLDPVFRALAPPVDSAPR
                     TPGREKADGTAERGYLNCGPNGAGHFVKMVHNGIEYGLMAAYAEGLNILRHAGVGKLQ
                     HERDAETTPLQDPERYLYDFNLADIAEVWRRGSVIASWLLDLTAAALLDDPVLAKFAG
                     RVSDSGEGRWTIHAAVDETVPAPVLTAALYQRFSSRGEADFADKVLSAMRFAFGGHLE
                     KK"
     gene            2168..2812
                     /locus_tag="VF357_07205"
     CDS             2168..2812
                     /locus_tag="VF357_07205"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7523972.1"
                     /translation="MTRVPSDAYEQPLGDGKETDERKPEIWIVRHGETEWSRRGRHTG
                     RTDAPLTAVGEWEAMALRSRLGGRRFALVLSSPLSRAWETCRLAGYGDVAGADDDLRE
                     WDYGTYDGRTTAEIQREMPGWTIWDGEVPGGETAAEVGRRADRVIRRATEADGDVALF
                     AHGHLLRVLSARWLGLGPNEGRLFALGTSSIGVLGYERDVRVIRTWNLTAETGP"
     gene            2915..3367
                     /locus_tag="VF357_07210"
     CDS             2915..3367
                     /locus_tag="VF357_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7523973.1"
                     /translation="MNDIRRIMVVATDIKHSRETVHSGVSLARKYDAELVVLHVEHDP
                     FGVDGWNLPFLSVDEEYKALLKEAKKNLEKVIQVESGSGLQVKEVIREGEPIGAIRKV
                     VEEEKIDLLILPAHEESRLEHFLFGRINEAIVRRLPCSILLLKQEADA"
     gene            3439..4275
                     /locus_tag="VF357_07215"
     CDS             3439..4275
                     /locus_tag="VF357_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7523974.1"
                     /translation="MNTMEFSFLVWLGGVAAGFLGSLTGLGGGVVIVPLLALGFGVDI
                     RYAIGASLVSVIATSSGAAAAYVKEGYSNLRIGMFLEIATTIGALAGAFLAARMSTSA
                     IAVIFGVVLFYSAYLSNRSRPERSEAARPDPLATFLRMDSIYPTVDGPQSYSVHNVPG
                     GFGLMFIAGGLSGLLGIGSGAVKVLAMDQVMRIPFKVSTTTSNFMIGVTAAASAGVYL
                     SRGYIDPVLAMPVMLGVLAGSMMGARVLTRAKSRTLRIVFSLVILALGVELIYNGLTG
                     RL"
     gene            4289..4666
                     /locus_tag="VF357_07220"
     CDS             4289..4666
                     /locus_tag="VF357_07220"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEX7523975.1"
                     /translation="MRAWTDERVEGVIGILLRWGVILAAAVVLSGGAMYLVRNGSTIS
                     DYRVFRGEPSDLRDVSGIVAAAVSWRSRGLIQFGLLLLIAVPVARVAFSVVAFALQRD
                     RTYVVVTLIVLAVLLYGLARAVP"
     gene            4698..7100
                     /locus_tag="VF357_07225"
     CDS             4698..7100
                     /locus_tag="VF357_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX7523976.1"
                     /translation="MGKDADRKEAFGGPGIPPRWTHAAKDGVGTAYASSSRLWFTIWN
                     GVVTEVYYPTVDRPQLRDLQFLVTDGESFFHEEKRHLRSMTERLSHHVLGYRITNSDP
                     EGRYSIVKEVLSDPHLPCLLVNTTLAGEESFLKRLRLYALCAPHLGMGGWGNNASVEE
                     IAGRVILTAEKNGVWLALAATVPFSRLSCGYVGQSDGWTDLAVDFRMDWEFDRATDGN
                     IALTGELDLGDRREFTMGLAFGDGLHNAATTLLESLAVPFGEQRERYMDQWNRSCRHL
                     LPLETAASDGGDLYHGSYSLLLAHEDKTYPGAFIASLSIPWGESKDDDDRGGYHLVWT
                     RDMVNTATGLLAAGNTETPLRALIYLAVSQHEDGGFSQNFWIDGEPYWRGVQLDEVAF
                     PILLAWRLLRKKGLGNFDPYPMVMGAARYLILHGPATPQERWEETGGYSPSTIASNIA
                     ALVCAASFARERGDGASAAFLEEYADFLECHVEAWMVTTEGTLVPGIPRHYIRILPAD
                     VNDPFPPEDPNRGILALANQPPGSRWRYPGKEIVDGGFLELVRYGIRKADDPVIVDSL
                     RVLDAVLKVDTPNGPCWRRYNHDGYGQREDGGPFVGHGKGRAWPLLTGERGHYELAAG
                     RPPGPYLQAMEGFASPTGLLPEQVWDEPDRPDLYMYLGRPTGSARPLMWAHAEYIKLL
                     RSAADGKVFDLVPEVADRYLSGGKVCKPMEIWKQNRQVSEVRQGFILRIQAGAPFLLH
                     WTRDDWGATEDSHSTPTALGIDYLDVDILPQQGGRIRFTFFWTDTGRWEGRDFEVAVR
                     GS"
     gene            complement(7127..>8045)
                     /locus_tag="VF357_07230"
     CDS             complement(7127..>8045)
                     /locus_tag="VF357_07230"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX7523977.1"
                     /translation="AEKSWKASSNREGRGESAYNTSMIPYFPQPEYHLFGPVTVHAFG
                     AIVALAVIVGWQMAFARARTKGLDPELFEDLLLYVVLSGFVVAHLYSVLAYFPREAME
                     DPLLLLKFWEDISSFGGFAGGLLGLWLFFRFKARDVDAAARLRYLEIIAYVFPFAWAI
                     GRIACTVAHDHPGTVTTFPLGISLKSPEAQAYIAFFYREAGRLAELPPPAELARMAFH
                     DLGWYEFLYMSCLMLPAFLVLDRKPHPPAFFLIAFPLLYVPARFFLDFLRISDARYFG
                     LTPGQYAGIAVFLAAVFFMVRISRREEKE"
BASE COUNT         1432 a         2537 c         2710 g         1366 t
ORIGIN      
        1 cagcgtggct ctctggaccg tgcgatcgag caccagttgt ccggaggtcc ggagacgggg
       61 cgattcgagc gacatccgcg cgaccgagac gaccgttctc gcctcgtcga tctcgaccgt
      121 tccgccgatc cggagaccgg acacgcccgt gcttccgttc cctcgacgca ccgacaggga
      181 ccggctctcc ccgcgcactt ccgccttcag cgtgcggaac cgctcgatct cgaagccggc
      241 gccaaggtcg gcgcgtccgg cggccatctg ccgccagccc tccggcagga acgattctgc
      301 gaagggtgcc aggtccagcc cgaccagctc gatcgtcccc cgcccgctca actccgccgc
      361 ctccagacgt ccgttcaggg agaggtttcc ccagagattc gatgcgcagc ggagatcgac
      421 atcgagccgt ttcggcggca ggatgatccg tccctcgatc tcccggagcg cacagagggg
      481 gcggggcccg agggagagcg cgacgcggcc acgatgcacc tcgaaggtca ttcccggcgc
      541 gtcgaccgag agggtggaca acagggccgc gatctgctcc cggatttccg cgagggacgg
      601 aggtttctct tccttcgccg accgcggaat ctccacagcg aggtccggcg cgtccgcgcg
      661 gatcttcgcg aggtgatacc ctccccggac cagcgggaac agggaggggg aaagggtcag
      721 ggattcgacg gttccggcgg cccggtgggg gatggccaac tccagccgcc ggacgacgat
      781 ccgggggcgg gggaaccaga cgaggtccag gcgttcatac cgcaccgttc ccccggcggc
      841 gcgggagacg cgggccagga ttccggcctt gaccgcctcc ccgttgatcc accggggcat
      901 caggaccgtc agcgcaccca ggaggacggc gaggagcccg aaggctcccg cgatccccag
      961 cgtgattttc ttccagccgc tcatcgcttg agacagaata tcgcgtcccg cgaattcccg
     1021 cgatgcacca tcgcgctgtt tacggcatcc caataccgga aaccggattg cggaagcgag
     1081 cccccattcc gtaaactcga tcctggcgac ctgcacccgg ggggaagacc cggcagcggc
     1141 gtgccaaggg aggattgacc atggaacttg gaatgatcgg cctcggccgg atgggcgcca
     1201 acatggtgcg gcggctcatg cgcgccgggc acaggtgcat cgtgtacgac acctctgcgg
     1261 aagcggtggc ggcgctcgcg aaggaagggg cgacgggcgc cgactcgctc gacgatttcc
     1321 tgagcaggct ctcgaaaccg cgcatcgtct ggatgatggt cccggcggcc gtcgtggacc
     1381 gggccatcgc agacctcgcc ccgcggcttg cgcgaggcga cgtcctcgtg gacgggggga
     1441 attcctacta cgtggacgac atccggcggg cgaaggacct ctccgcgaag gggattcatt
     1501 acgtggacgt ggggacgagc ggcggcgtgt tcggcctcga gcgcggatac tgtcagatga
     1561 tcggcggcga gccggagatc gtgaagcgcc tcgacccggt cttcagggcg ctggcgccgc
     1621 ccgtcgactc ggccccgcgc acgcccggac gggagaaggc cgacggcacc gccgagcgcg
     1681 gctatctgaa ctgcggaccg aacggcgccg gacacttcgt gaagatggtg cacaacggaa
     1741 tcgagtacgg cctcatggcc gcctacgccg aggggctcaa cattctgcga cacgccggcg
     1801 tgggcaaact tcagcatgaa cgggacgcgg agaccacgcc gcttcaggac ccggagcgtt
     1861 acttgtacga tttcaacctg gccgacatcg ccgaagtctg gcgccgcggg agcgtcatcg
     1921 cctcctggct cctcgacctg accgccgccg ccctgctcga cgaccctgtc ctcgccaagt
     1981 tcgccggccg ggtttccgac tccggcgagg ggcgatggac catccacgcc gccgtcgacg
     2041 agaccgtccc cgctccggtg cttaccgccg ctctctacca gcgtttttcc tctcgcggcg
     2101 aggcggattt cgccgacaag gtcctctccg ccatgcgctt cgccttcggc ggccacctgg
     2161 agaagaaatg acccgcgttc cgtcggatgc gtacgagcag cccctcggcg acgggaagga
     2221 gacggacgag cgaaaacccg agatctggat cgtccgacac ggcgagaccg agtggagccg
     2281 aaggggacgg cacaccgggc gcacggacgc acccctcacg gccgtcggcg agtgggaggc
     2341 gatggcgctc cgcagcagac tcggcggcag gcgcttcgca ctcgtactgt cgagtccgct
     2401 ttccagggct tgggagacgt gccgcctcgc gggatacggg gacgtcgccg gggcggacga
     2461 tgacctccgg gaatgggact acgggaccta cgatgggcga accaccgccg agattcaacg
     2521 cgagatgccg ggatggacga tctgggacgg tgaggtcccg ggcggagaaa cggccgcgga
     2581 ggtgggccgc cgcgctgacc gggtcatccg gcgggcaacg gaggccgacg gcgacgtcgc
     2641 cctcttcgcg cacggacacc tcctgcgggt cctctctgct cgctggcttg ggctcgggcc
     2701 gaacgagggc cgtctcttcg ccttggggac ctcctcgatc ggggtgctcg gctacgagag
     2761 agatgttcgc gtgatccgca cctggaacct caccgccgag acggggccgt gagactcgga
     2821 ggccaggagg aaggaacggt ttggacggga accgaactgt ctgaaaacgg aaatgtgtgc
     2881 cggacggcat tctcaatatc gtaagggggt cgacatgaac gacattcggc ggatcatggt
     2941 ggtggccacg gacatcaagc acagccggga aacggtgcac tccggggttt ccctggcgag
     3001 gaagtacgac gcggagttgg tggttctcca cgtggagcac gatcccttcg gggtggatgg
     3061 ctggaacctg ccattcctgt ccgtcgatga ggaatacaag gcgctgctca aggaagcgaa
     3121 gaagaacctg gagaaggtta tccaggtcga gtcggggagc ggattgcagg taaaggaggt
     3181 gatccgggaa ggggagccga tcggcgcaat ccggaaagtg gtggaggagg aaaagatcga
     3241 tcttctcatt ttgccggccc acgaggagag ccggctcgag cacttcctgt tcgggcgcat
     3301 caacgaagcg atcgtccgca ggctgccctg ctccatcctc ctgttgaaac aggaggcgga
     3361 cgcctgaacg ggatgttccc cacggcggcc ccgttcctcc gttccaagta ttgtggatag
     3421 cggacccgga gatggttcat gaacaccatg gaattctcgt tccttgtctg gctggggggc
     3481 gtggcggcag gattcctcgg ttccctgacg ggattggggg gcggtgtcgt catcgtgcct
     3541 ctcctggcat taggttttgg ggtcgacatc cggtatgcca tcggcgcctc gctcgtatcg
     3601 gtcatcgcca cctcttccgg ggccgcggcg gcctacgtca aggaaggata ctccaacctc
     3661 cggatcggga tgttcctgga gatcgcgaca accatcgggg ccctggccgg ggctttcctg
     3721 gccgccagga tgtccacttc cgcgattgcg gtcatcttcg gggtcgttct cttctactcg
     3781 gcgtacctgt cgaaccgctc ccggccggaa cgatccgaag ccgcccgtcc cgaccctttg
     3841 gcaaccttcc tgaggatgga ttcgatctac ccgacggtcg atggcccgca gtcctacagc
     3901 gtgcataatg tccctggcgg attcggcctg atgttcatcg ccggagggct ctccggtttg
     3961 ctcgggatcg ggtcgggggc cgtaaaggta ctcgccatgg accaggtcat gcgcattccg
     4021 ttcaaggttt cgacgaccac cagcaacttc atgatcggcg tgacggcggc cgcaagcgcc
     4081 ggagtttact tgagccgcgg atatatcgac ccggtcctgg ccatgccggt catgctgggg
     4141 gtgctcgccg gatccatgat gggggcaagg gtattgaccc gcgccaagtc ccggaccctg
     4201 cgcatcgtct tcagcctggt tatcctcgcg ctcggagtgg agctcatcta caacggcctg
     4261 acggggaggc tttaggagag tcccgaatat gcgtgcatgg accgatgagc gtgtggaggg
     4321 ggtgatcggg attctcctgc ggtggggggt catcctggcg gcagcggtgg tcttgtcagg
     4381 aggcgccatg tatctggtcc gcaacgggtc cacgatatcc gattaccggg tttttcgcgg
     4441 ggaaccgtcg gacctgagag atgtgtcggg aatcgtggcg gctgccgttt cctggcgcag
     4501 tcgaggtttg atccagttcg ggctcttgtt gctgatcgcc gtccccgtgg cacgggtggc
     4561 cttctccgtg gtcgccttcg ccctgcaaag ggaccgcacc tacgtcgtgg tgaccctgat
     4621 cgtcctcgcg gttcttctgt acggcctggc gagggcggtc ccgtaaaccg tcgttccaat
     4681 cgccgaaagg agtcgcgatg ggaaaggatg ccgatcggaa agaggcgttc ggagggccgg
     4741 ggatcccgcc ccgatggacc catgccgcaa aagacggggt cggcacggcc tatgccagct
     4801 ccagccggct ctggttcacc atctggaacg gggtcgtcac cgaggtctac tatccgaccg
     4861 tggatcggcc gcagctccgg gatctccagt tcctcgtcac ggacggggag agtttcttcc
     4921 acgaggagaa gcgtcatctc cgttccatga cggaacgtct ctctcatcat gtccttgggt
     4981 acaggatcac gaactccgac ccggaggggc gctattccat cgtcaaggag gtcctctccg
     5041 acccccatct gccatgcctc ctcgtgaaca cgaccctcgc cggggaggaa tccttcctga
     5101 aacgacttcg actgtacgcc ttgtgcgccc cacacctcgg gatggggggt tgggggaaca
     5161 acgccagcgt agaggaaatc gcgggccgtg taatcctcac cgccgagaag aacggggtct
     5221 ggctcgcgct ggccgcaacc gtccccttct cgcgtttgtc ctgcggctac gttggtcaaa
     5281 gcgatggctg gacggacctt gcagtcgact tccgaatgga ctgggagttc gaccgtgcca
     5341 cggacggaaa tatcgccctc accggcgagc tggacctggg cgatcgccgg gagttcacga
     5401 tggggctcgc tttcggcgat ggcctccaca acgcggcgac gacgctcctg gaatctctcg
     5461 ccgttccctt cggggagcag cgggaacggt acatggacca gtggaaccgg tcgtgcaggc
     5521 acttgctacc gctcgaaacg gcggcgtccg acggcgggga cctctatcac gggagctaca
     5581 gcctccttct cgcccacgaa gacaagacgt atccgggtgc cttcatcgcc tccctgtcca
     5641 tcccctgggg agaatcgaag gacgacgatg accgtggggg atatcacctc gtatggacga
     5701 gggacatggt caacacggcc acgggcctcc tcgccgcggg gaacaccgag acgccgttgc
     5761 gtgccctgat ctacctggcc gtcagccagc acgaggacgg cggattttcc cagaacttct
     5821 ggatcgacgg cgaaccgtac tggcgtgggg tccagctgga cgaggtcgcc ttccccatcc
     5881 tccttgcgtg gcgtctgctg cgcaagaagg gtctcgggaa cttcgacccc tatccgatgg
     5941 tgatgggggc ggcacggtac ctgattctcc atggaccggc gaccccgcag gagcggtggg
     6001 aagagaccgg cggctattct ccgtcgacca tcgcctccaa tatcgcggcg ctcgtctgcg
     6061 cggcctcctt cgcccgcgaa aggggagatg gggcgtcggc cgcatttctc gaagagtacg
     6121 ccgacttcct cgaatgccac gtcgaagcat ggatggtgac caccgagggg acgctcgtcc
     6181 ccgggatacc gaggcattac atccgcatcc tccctgcgga cgtgaacgat ccgttcccac
     6241 ccgaggaccc caaccgcggc atccttgcgc tggccaacca gcccccgggt tcccgctggc
     6301 ggtatccggg gaaggagatc gtcgacggag ggttcctgga gcttgtccgg tacgggatcc
     6361 ggaaggcgga cgatcccgtc atcgtggact ccctgcgcgt gctggatgcc gtgctaaaag
     6421 tggatacgcc gaacggtccg tgctggcgtc ggtacaacca cgacggatac gggcagagag
     6481 aggacggcgg gccgttcgtc gggcatggga aggggcgcgc gtggcctctg ctcaccgggg
     6541 agcggggcca ctacgagttg gccgccggac ggcctccggg cccgtatctt caggccatgg
     6601 aggggttcgc ctccccgacc gggttgctgc cggaacaagt atgggacgag ccggaccggc
     6661 ccgatctcta catgtacctg ggacggccca ccggttccgc caggccgctg atgtgggcgc
     6721 acgccgagta catcaagctg ctccggtcgg cggccgacgg caaggtgttc gacctcgtcc
     6781 ccgaagtcgc ggaccgctac ctctccggcg ggaaggtctg caagccgatg gaaatctgga
     6841 agcagaaccg ccaggtctcc gaggtccggc aggggttcat ccttcgcata caggcggggg
     6901 cgccgttcct cctccattgg acgcgggacg actggggcgc tacggaagat tcacactcca
     6961 ccccgaccgc gttggggatc gattacctgg atgtcgacat cctcccccaa cagggcggtc
     7021 ggatccgctt tacgtttttc tggacggaca cggggcgctg ggaagggagg gatttcgagg
     7081 tggccgtccg tggatcgtga gcgcaggact ccgacccatt gcacgcctac tccttctctt
     7141 cccgccggga aatgcgaacc atgaagaaga ccgcggcgag gaaaaccgcg atcccggcgt
     7201 actgcccggg agtcagcccg aagtacctgg catcgctgat gcgcaggaaa tccaggaaga
     7261 agcgggccgg gacgtagagg agcgggaagg cgatcagaaa gaacgccggc ggatggggtt
     7321 tccggtccag cacaaggaag gcggggagca tcaggcacga catgtagagg aattcatacc
     7381 atcccaggtc atggaacgcc atcctcgcca gctccgcggg tggcggaagt tccgccaacc
     7441 ggccggcctc ccggtagaag aaagcgatgt aggcctgcgc ctccggggat ttcaggctga
     7501 ttcccagcgg gaacgtcgtg accgtgccgg ggtggtcgtg ggcgaccgtg caggcaatgc
     7561 gcccgatcgc ccatgcgaac gggaagacgt atgcgatgat ctccaggtat cgcaaccggg
     7621 ccgccgcatc gacatcccgc gctttgaaac gaaaaaagag ccagagcccg aggagtcctc
     7681 cggcgaatcc cccgaaggag ctgatgtcct cccagaactt cagaagaagg agcgggtctt
     7741 ccatcgcctc ccgagggaaa taggcgagga cggaatagag gtgggcgacg acgaagccgg
     7801 agaggacaac gtacaacagg aggtcctcga aaagttcggg gtcgaggccc ttcgtgcgtg
     7861 cgcgggcaaa ggccatctgc cagccgacga tcacggcaag ggcgacgatc gccccgaacg
     7921 cgtggacggt gaccgggccg aacaggtggt actcgggttg cgggaaatac gggatcatgg
     7981 atgtattata tgcggactcc ccccgcccct ccctgttgga cgacgccttc cacgattttt
     8041 ccgcc
//
LOCUS       DASVJL010000342         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1368984, whole genome shotgun sequence.
ACCESSION   DASVJL010000342 DASVJL010000000
VERSION     DASVJL010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368984"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /gene="istA"
                     /locus_tag="VF357_07235"
     CDS             complement(<1..1051)
                     /gene="istA"
                     /locus_tag="VF357_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX7523978.1"
                     /translation="MRKIKEALRLKFACGLTDRQVARSIGVARSTAAEYFRRFTASGL
                     PYPLPEEIDDASLAARLFPPPSSLPPPLSRSLPDFAVTQRELRKKGVTLRLLWEEYRE
                     NHPDGYQYSRFCDHYRLWLRRRDLVMRQEYKAGQKMFVDYAGKTMPVVDRFTGEVHDA
                     QIFVSVLGASNYTFCEATWTQSLPDWTASHVRAFEYFEGSCDELIPDNLKSAVTTPSF
                     YDPVLNPTYEEMAAHYGTAVLPARIRRPRDKAKVEAGVLLVTRWILARLRNRIFFSLV
                     ELNEEIRRLLEALNNRPFRKMEGSRKSLFETMEKPALKALPERPFEYGEWKRATVNID
                     YHIDVERHYYSVPYTL"
BASE COUNT          287 a          422 c          519 g          292 t
ORIGIN      
        1 gcagggtata cgggacgctg taatagtgcc tctcgacgtc gatgtggtag tcgatgttga
       61 ccgtggccct cttccactcg ccgtattcga aggggcgctc cgggagggcc ttcagggcgg
      121 gtttttccat cgtctcgaag agggacttgc gcgatccttc catcttccgg aagggacggt
      181 tgttgagcgc ttcaaggaga cgacggatct cctcgttgag ttcgaccagg gagaagaaga
      241 tccggttgcg caggcgggcc aggatccagc gggtcaccag aagcactccc gcttccacct
      301 tcgccttgtc ccggggcctg cggatcctgg cgggcaggac ggcagtcccg tagtgagcgg
      361 ccatctcttc gtaggtgggg ttgagaacgg gatcgtagaa ggacggcgtc gtgacggcgc
      421 ttttcaggtt atcggggatc aactcgtcgc aagatccctc gaagtattcg aaggcccgca
      481 cgtgggaggc ggtccagtcg ggaaggctct gggtccaggt agcctcgcag aaggtgtagt
      541 tggaggcccc caggacggag acgaagatct gggcatcgtg gacctcgccg gtgaaccggt
      601 cgacgacggg catcgttttt cccgcgtagt ccacgaacat cttctgcccg gccttgtact
      661 cctgccgcat caccaggtcc cggcgacgca gccagaggcg gtagtgatcg cagaaccggc
      721 tgtactggta cccgtcggga tgattttccc ggtactcctc ccacaacagc ctcagggtca
      781 ctcccttctt gcggagctct cgttgcgtga cggcaaaatc gggcagggaa cgggacagag
      841 gaggcggcag ggacgagggg ggcgggaaca gacgcgccgc aagagacgcg tcgtcgatct
      901 cctcgggcag cggatacggc agccccgagg cggtgaaccg gcggaagtac tccgccgcgg
      961 tgctgcgcgc cacacctatg ctgcgcgcga cctggcggtc ggtcaggccg caggcgaact
     1021 tgagacgaag cgcttccttg atcttgcgca cggggattct ccctcggggc atccagaacc
     1081 tcctccacga tgtatgagaa ggcccaagga taccccccgg tggaataccc cggcgtcgga
     1141 taggctcctt cggggaggat cagcccggaa cggtccggat caattccgac ccacccagga
     1201 aaagtggccg gaatcggctc ggaacgccgg ccgaattccc ctcggaacgg tggccggatt
     1261 cacgtcggaa cagtggccgg tttggcctcg gaatggtggc cggtttgcgt cggaatatgc
     1321 acgcctgtga tggaaggacg ttcgtggggg tacatagaca agaaaggctc atctgttatt
     1381 agcccgcggt atcaatggac ttgtgggttt tcagagggac ttgcttttgt gagaacagag
     1441 aacggcggcg gatacatcga tcggagcggg gagttggtta ttcgtgcgga taattgggat
     1501 atcggggaca cttttcgagg
//
LOCUS       DASVJL010000343         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1275885, whole genome shotgun sequence.
ACCESSION   DASVJL010000343 DASVJL010000000
VERSION     DASVJL010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275885"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VF357_07240"
     CDS             complement(<1..903)
                     /locus_tag="VF357_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7523979.1"
                     /translation="MPELSKERLERYLTTLFHAPATITALTSLRESKASGAAKAYGYG
                     HPVKVEFTIGASLRAAVLETTSPGPFGHEHMADRARMMLWDHGAYNALPKHVRSLDVG
                     AFDREGNLLSVASAEEFFLLVEHVEGAGYILDLARLQEGAPLRDLDLSRADALCDYLV
                     GIHSVRGPDPGLYVRKIRELLGDGECIMGVCDGYPLPHGFITAALLEEIERRCVAWRW
                     RLKGRTDRMRQVHGDYHPFNILFREGTDFTVLDRSRGEWGEPADDVTALTGNYLFASL
                     QATGRLEGPFETLFRRFWDRYIERT"
     gene            1012..1236
                     /locus_tag="VF357_07245"
     CDS             1012..1236
                     /locus_tag="VF357_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX7523980.1"
                     /translation="MERGLVALAITGMTCGHCVAAVKKALAAVPGVEAVEVTLTPPRA
                     VVACDPSKTTVEMLVKATGEEGYPSSPAAG"
     gene            1249..1653
                     /locus_tag="VF357_07250"
     CDS             1249..1653
                     /locus_tag="VF357_07250"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEX7523981.1"
                     /translation="MFEWTERWSVGVDTIDAQHRELFAAINSLLREEGKPDARELVKV
                     LDYLEDYVNNHFGLEEIYMRRLSYPGFPSHKGEHVAFINDFYDLRDEYDDNGATPELA
                     DKMGRYMGDWLVNHIGKIDKALGGFLRDKGMK"
     gene            complement(1659..2159)
                     /locus_tag="VF357_07255"
     CDS             complement(1659..2159)
                     /locus_tag="VF357_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523982.1"
                     /translation="MEPVSVALTVVHLLAVIAWLGGMIFHIVVLDPVFRKNEVSFQSA
                     YLLALMEGRFRKLVGTSIALLLASGAAKGYLLLGTTDALLHTAVGRYVLLKVGLTAAM
                     LVIFAVCPKTRSCSPIPGVCDVASEGILSGKTDRTIRERSKVILPKVALVLGLAAFVV
                     AVLLRG"
BASE COUNT          426 a          728 c          766 g          366 t
ORIGIN      
        1 cgtccgctcg atgtaccggt cccagaatct ccggaacaac gtttcgaagg ggccttcgag
       61 gcggcccgtc gcctgcagcg acgcgaacag gtagttcccc gtgagggcgg tcacgtcgtc
      121 cgccggttcc ccccactcgc cgcgcgaccg gtccagcacg gtgaagtcgg ttccttcccg
      181 gaagaggatg ttgaaggggt ggtagtcccc gtgcacctgg cgcatccgat cggtgcgtcc
      241 tttcaggcgc cagcgccagg caacgcaacg ccgctcgatc tcctcgagca acgcggcggt
      301 gatgaatccg tgggggaggg gatagccgtc gcacactccc atgatgcact cgccgtcccc
      361 cagcagttcg cggatcttgc ggacgtagag cccagggtcc ggcccccgga cggagtggat
      421 cccgacgagg tagtcgcaca gggcgtccgc ccgcgagaga tccaggtcgc gcagcggggc
      481 cccctcctgg aggcgcgcga ggtccaggat gtaccccgct ccttcgacgt gctcgaccag
      541 gaggaagaat tcctcggcgc tcgcgacgga gaggagattc ccctcgcggt cgaacgcgcc
      601 cacgtcgagg gagcggacat gtttcgggag agcgttgtac gccccgtggt cccacagcat
      661 catccgggcg cgatccgcca tatgctcgtg accgaaggga ccggggctcg tcgtttcgag
      721 cacggcggct cgcagcgaag cgccgatggt gaactcaacc ttcaccgggt gaccgtaccc
      781 gtatgccttg gccgcccccg acgccttcga ttcacggaga gaggtcaggg cggtgatggt
      841 cgccggcgca tgaaacaacg ttgtcaggta ccgctccagg cgttccttcg agagttccgg
      901 catcggcgcg cctcgtgaca agtatcgtcc cgttctccat tatcctctgc agtctactat
      961 ataatattcg catatccgga gatgaccgac gaacagggaa ggtgagaggc gatggagagg
     1021 ggacttgtgg cgctggcgat cacggggatg acatgcggcc attgcgtcgc cgcggtgaaa
     1081 aaggcgctcg cggcggtacc cggggtcgag gcggtcgagg tgaccctgac acctccgcgc
     1141 gcggtggtcg cctgcgatcc ctcgaagacg accgtggaga tgcttgtgaa ggcgaccggg
     1201 gaggagggat acccctcttc cccggcggcc ggataaccgg aggcgatctt gttcgagtgg
     1261 acggaacgat ggtcggtggg cgtggacacc atcgatgccc agcatcggga acttttcgcc
     1321 gcgatcaact cgctgctgcg ggaggagggg aagcccgacg cgcgggaact ggtgaaggtt
     1381 ctcgactatc tcgaggacta cgtcaacaac catttcgggc tggaagagat ctacatgcgc
     1441 cgcctctcct acccgggatt cccctcccac aaaggggagc acgtggcgtt catcaacgac
     1501 ttctacgatc tgcgggacga gtacgacgac aacggcgcca cccccgagct ggccgacaag
     1561 atgggccgct acatgggcga ctggctggtc aaccacatcg ggaaaatcga caaggcgctc
     1621 ggggggttcc tgcgcgacaa ggggatgaag tagcgccgct acccgcggag caggacggcg
     1681 acgacgaagg cggccagtcc gaggaccagc gccaccttcg ggaggatcac cttggaccgc
     1741 tcccggattg tccggtccgt cttgccggaa aggattcctt ccgaggcgac gtcgcagact
     1801 cccgggatgg gggagcacga cctggtcttc gggcacaccg cgaagatgac gagcatcgcg
     1861 gccgtcagcc cgaccttcag gaggacgtac cggccgaccg cggtgtgaag gagcgcgtcg
     1921 gtcgtcccca gcagcaggta ccccttcgcg gcgcccgagg cgagaaggag ggcgatcgag
     1981 gtccccacca gtttccggaa ccggccttcc atcaaggcga gaaggtaagc gctctggaag
     2041 ctcacctcgt tcttccggaa caccgggtcc aggacgacga tgtggaagat catccctccc
     2101 agccatgcga tgacggccag caggtggacg acggtcagcg cgaccgatac gggttccatg
     2161 cgccctcctt tccccgggat ccgcagatca cactacaccg gccgttgccg accgatttga
     2221 cccacgtcaa tcgcgcgcgg aattcccttc gccgaggaag aggtcgggga ggagcggagc
     2281 gcccgg
//
LOCUS       DASVJL010000344         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1650505, whole genome shotgun sequence.
ACCESSION   DASVJL010000344 DASVJL010000000
VERSION     DASVJL010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650505"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VF357_07260"
     CDS             complement(<1..1007)
                     /locus_tag="VF357_07260"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEX7523983.1"
                     /translation="MTPRLPTREILWWSLRAHWALALALFALFTDAARWALLLAAGAW
                     VAGVTMDRADTPRAALSRLGSPIVAIFFAAAAADLLFGSRDLLGSLSLLVLGVQSVKF
                     LLPKGSRDGWQLCAVALLEFLAAAAGTDALAFALFAFLFFVASAGAMGALHDQEAGEG
                     GRPSGGYAVPARAAAGVLLAAGVGGFLASAVLFAVIPRLEFRQIFERFGRARGVVGFS
                     ETISLREVTGIKSDPRVVARVEFPELPPGVLPANLYLRGAVYSRYENGVWRHGGATPL
                     PVPRAGFQHFVGDAAVPDSTADITLEAADHPALFTYGHPTLIEGAFSPVLSDGEGNLS
                     LA"
     gene            complement(1004..1948)
                     /locus_tag="VF357_07265"
     CDS             complement(1004..1948)
                     /locus_tag="VF357_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7523984.1"
                     /translation="MRPVTRRFRLPRHLRIPFAGRAFLLVTLGIGVAAVNTGNNLLYL
                     SLSLNLSLILLSGVLSEGTLRHVALKVRLASEAFAGGETFLAVTCSAEAKRFPGISLV
                     AILRAGEEPTTVRFPDIAPGTSVTRVVPFRPSLRGELDSIHASLSTRFPFSLFEKSVE
                     LDIPGGVIVYPRPSPPASRREDLPVAAPSGRPFLSGRVGAFPRGVRGHLPADPVRDIH
                     WKATARTGRWMVKEREGEATPGIDLRVEETGTPEAFEARLSDACGAVLELDRRAVPFR
                     LRIGDRLCAEAHDPNRRSKALEALAKARFDPSSPVTPS"
     gene            complement(1945..>2256)
                     /locus_tag="VF357_07270"
     CDS             complement(1945..>2256)
                     /locus_tag="VF357_07270"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523985.1"
                     /translation="LRGEIDRVTVHDAVVDYIQRLAAATRAHPAIRLGASPRGAIGLK
                     STSQALALLSGRDHVTPGDVRRAAVPVLCHRVFPKGDAAGSEAAQLIIEEVLSVVPSP
                     L"
BASE COUNT          399 a          753 c          832 g          272 t
ORIGIN      
        1 gcaagggaaa ggttcccctc cccgtcggag aggacggggg agaacgcgcc ttcgatcagc
       61 gtcggatgcc cgtatgtgaa gagtgcgggg tggtccgccg cctcgagggt gatgtccgcc
      121 gtggaatccg ggacggccgc atctcccacg aagtgctgga atccggccct tggaacggga
      181 agaggagtcg cgccaccgtg gcgccaaacc ccgttctcat accgggaata caccgcgccc
      241 cgaaggtaga ggttcgccgg taggaccccg ggaggcagtt ccgggaattc caccctggcc
      301 accacccgcg ggtccgattt tatgccggtc acctcgcgga gcgagatcgt ctcggagaaa
      361 ccgacgacgc cccgagcgcg tccgaaccgc tcgaaaatct ggcggaattc gagacgcgga
      421 atgaccgcga acaggaccgc cgaggcgaga aacccgccga ccccggcggc aaggagcacc
      481 cccgccgcgg cccgtgccgg gacggcgtat cctcccgacg gacggccccc ctctcctgcc
      541 tcctggtcgt gaagggcccc cattgcgccg gcggaggcga cgaaaaagag gaacgcgaag
      601 agggcgaagg ccagcgcgtc ggttccggcg gcggcggcga gaaactcgag gagggcgacg
      661 gcgcacagct gccatccgtc ccggcttccc ttcgggagaa ggaacttcac cgactgcacc
      721 ccgagcacga gcagcgaaag ggagccgagg aggtcgcggc ttccgaacag gaggtcggcc
      781 gcggccgcgg cgaagaagat cgcgacgatc ggcgatccga gccgggagag ggcggcgcgc
      841 ggcgtgtccg cccggtccat cgtcacgccg gcgacccatg cgccggcggc gagcaggagg
      901 gcccatcgcg ccgcgtccgt gaagagggcg aagagggcga gggcgagcgc ccagtgggcg
      961 cgcagggacc accagaggat ctcccgtgtc gggagacgag gggtcatgac ggcgtcaccg
     1021 gcgacgacgg gtcgaaccgc gctttcgcca aggcctcgag ggccttcgac cgccgattcg
     1081 gatcgtgcgc ttctgcgcaa agccggtctc cgatccggag tcggaacggg acggcccgcc
     1141 gatcgagttc gagcacggcg ccgcacgcat ccgaaagccg ggcttcgaat gcctccggcg
     1201 tcccggtctc ctccacgcga aggtcgatcc cgggcgtggc ttccccttcc cgttccttga
     1261 ccatccagcg tcctgtccgc gccgtcgcct tccagtggat gtcccgcacg gggtccgcag
     1321 ggagatgccc ccgcaccccc cgggggaagg cgccgacccg cccggacaga aatggtcgtc
     1381 ccgacggcgc cgcgaccggg aggtcttcgc gccgcgaagc gggcggggac ggccgggggt
     1441 acacgatgac gccgccggga atgtcgagtt cgaccgattt ctcgaacagg gaaaacggga
     1501 accgcgtcga gagcgaggca tggatcgaat cgagttcccc ccgtagggaa ggtcggaacg
     1561 ggacaacgcg ggtgacggac gtcccggggg cgatgtccgg gaagcggacc gtcgtgggct
     1621 cttctccggc ccgaaggatc gcgacgaggg agatcccggg aaaccgtttc gcctcggccg
     1681 aacaggtgac ggcgaggaac gtctcccccc ctgcgaacgc ctccgaggcg agccggacct
     1741 tcagcgcgac gtgccgcagc gttccctcgg acaggacgcc cgacaggagg atcagggaca
     1801 ggttgaggct caaggagaga tagaggaggt tgttccccgt gttgacggcg gcgacgccga
     1861 tcccgagggt gacgaggagg aacgccctcc ccgcgaaggg gatccggagg tgccggggaa
     1921 ggcgaaaccg gcgcgtgacg ggcctcacag cggcgacggc accaccgaga ggacctcctc
     1981 gatgatcaac tgggcggctt ccgacccggc ggcgtcccct ttcgggaaga cgcggtggca
     2041 gaggaccgga accgccgccc ggcggacatc cccgggggtc acgtggtcac ggccggagag
     2101 gagggccagc gcctgcgagg tgctcttcaa tccgatcgcc ccgcgggggg aggcgcccag
     2161 gcggatggcg gggtgggccc gggtcgccgc cgcgagccgc tggatgtagt cgacgaccgc
     2221 gtcatggacg gtcacccggt cgatctcccc ccggag
//
LOCUS       DASVJL010000345         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_712997, whole genome shotgun sequence.
ACCESSION   DASVJL010000345 DASVJL010000000
VERSION     DASVJL010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712997"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /gene="pnp"
                     /locus_tag="VF357_07275"
     CDS             <1..1111
                     /gene="pnp"
                     /locus_tag="VF357_07275"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX7523986.1"
                     /translation="QRIDSIMGDTTKSFMLHYNFPPFSVGEVKMLRGPGRREVGHGAL
                     AERAVSKVLPMNADFPYTVRVVSEVLESNGSSSMATVCGASLAMMDAGVPTSGAVSGI
                     AMGLIKEGNDVAVLSDILGDEDHLGDMDFKVAGTKNGVTAIQMDIKIGGVSREIMLSA
                     LQQAREGRLHILEKMNAVIDTHRADLSPHAPRIYVMMVKTDKIREIIGPGGKIIRGIQ
                     EQTGVKIDIDDDGTVKIAAVNADSARAAISIIEGITQSAEVGKVYEGRVRKIMDFGAF
                     VELFPGTDGLLHVSQISKHRVNVADCFKEGDDVTVRVLEVDRDGKIRLSHKEFEEEGR
                     FPEATGPPPGQDREKGDPGRDRGRDGGRGGGRGRR"
     gene            1151..2395
                     /locus_tag="VF357_07280"
     CDS             1151..2395
                     /locus_tag="VF357_07280"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7523987.1"
                     /translation="MTVARTLLDNGVAIVTEQVPWLRSATVGIWVPVGSRAETPVESG
                     VAHFIEHMLFKGTPRRRAVDISRAIESVGGTMNACTSREYTYFFAKSMEKDFPLLVDL
                     LTDIYRNSLFDEAELDRERGVILQEILMVDDTPEEYLHDYFNAAYWGGHALGFPVQGS
                     AESVGRFDRALLTGYFDDRFRRKGITVTTVGNLRHAVVAEAFERALSSLPLGESLIPV
                     PPTAPVRGTFLKRRPLEQVHLCLGAPGVSRRSERIYAMDVLNAVLGGSSSSRLFQQVR
                     EERGLAYSVGSSLSAYADAGIVDIYAGTGRETAAEVLSVAGDVVDALQRGGITDEEVS
                     FGKELIKGNTLLSLESTGYRMSCLAMNEMFLSRLEPPEAILDRVDAVTPEEVRALAAE
                     VLGRDRFTLAAVGDLPDAGLSF"
BASE COUNT          482 a          891 c          977 g          481 t
ORIGIN      
        1 gcagcggatc gactcgatca tgggggacac caccaagtcg ttcatgcttc attacaactt
       61 cccgcccttc agcgtcggag aggtcaagat gctgcgcggt cccggccgcc gcgaggtcgg
      121 gcacggggcg ctcgccgagc gcgccgtgtc gaaggtgctg ccgatgaacg ccgacttccc
      181 ctacacggtg cgcgtcgttt ccgaggtcct cgagtccaac gggtcctcct ccatggccac
      241 ggtgtgcggg gcgtccctcg cgatgatgga cgccggggtc ccgacctccg gtgccgtttc
      301 cgggatcgcc atggggctca tcaaggaagg gaacgacgtc gcggtcctct cggacatcct
      361 cggggacgag gatcacctgg gcgacatgga cttcaaggtg gcgggaacga agaacggagt
      421 gaccgccatc cagatggata taaagatcgg cggcgtcagc cgggaaatca tgctctccgc
      481 cctacagcag gcccgcgagg gtcggctcca catcctggag aagatgaacg ccgtcatcga
      541 cacgcaccgc gccgacctct ctccgcacgc tccgcggatc tacgtgatga tggtgaagac
      601 cgacaagatc cgtgagatca tcggtccggg aggaaagatc atccgcggga tccaggagca
      661 gacgggcgtg aagatcgaca tcgacgacga cggcaccgtg aagatcgcgg cggtgaacgc
      721 cgactcggcg cgggcggcca tctccatcat cgaggggatc acgcagagcg ccgaagtggg
      781 aaaggtctac gagggaagag tacgcaagat catggatttc ggcgccttcg tggaactttt
      841 cccgggcacc gacggcctgc tccacgtttc ccagatctcg aagcaccgtg tcaacgtcgc
      901 cgactgcttc aaggaggggg acgacgtcac cgtccgcgtc ctcgaggtgg accgggacgg
      961 gaagatccgt ctctcccaca aggagttcga ggaggagggg aggttccccg aggccacggg
     1021 cccgccgccg ggacaagatc gggagaaggg cgaccccggc cgggatcgcg ggcgggacgg
     1081 cggccgcggc gggggaaggg gacgtcgtta gtgcggcgtt tcggaacgcc gcgctagtgc
     1141 accgacgtcg atgacggtcg cccgtaccct cctcgacaac ggcgtcgcca tcgtcaccga
     1201 acaggtcccc tggctgcggt ccgccaccgt gggaatctgg gtgccggtgg gttcgcgcgc
     1261 ggaaacgccg gtcgaaagcg gcgtcgcgca tttcatcgag cacatgctgt tcaaggggac
     1321 gcctcgccgg agggccgtgg acatctcgcg ggccatcgaa tcggtgggcg gcacgatgaa
     1381 cgcctgcacc tccagggagt acacgtactt cttcgccaag tcgatggaga aggattttcc
     1441 ccttctcgtg gacctgctca ccgatatcta tcggaactcc ctcttcgacg aagcggaact
     1501 ggatcgcgag aggggcgtca tcctccagga gatcctcatg gtggacgaca cgcccgagga
     1561 gtatctccac gactatttca acgcggcgta ttgggggggg cacgcgctgg ggtttcccgt
     1621 gcaggggtcc gcggagagcg tggggcggtt cgaccgggcc ctccttacgg ggtatttcga
     1681 cgaccggttc cggcggaagg ggatcacggt gacgacggtg ggaaacctcc ggcacgccgt
     1741 cgtggcggaa gcgttcgaac gcgcgctttc ctcgctcccg ctcggggagt cgctgattcc
     1801 cgttcctccc acggcgccgg tccgggggac gttcctcaag cgaaggcccc tcgagcaggt
     1861 gcacctgtgc ctgggcgccc ccggcgtttc gcggcggagc gagcggatct acgcgatgga
     1921 cgtgctgaac gcggtcctcg ggggcagttc cagcagccgc ctcttccagc aggtccgcga
     1981 ggagcgcggg ctggcgtact ccgtcggctc ttccctgtcc gcctacgcgg atgccgggat
     2041 cgtcgacatt tacgccggca cgggtcggga gaccgccgcc gaggtgctct ccgtcgcggg
     2101 cgacgtggtg gacgctctcc agcggggcgg gatcaccgac gaggaggtct cgttcggcaa
     2161 ggaacttatc aagggaaata cccttctttc gctggaaagc acgggatacc ggatgtcgtg
     2221 ccttgcgatg aacgagatgt tcctctcccg cctggaacct ccggaggcga tcctcgaccg
     2281 cgtggacgcg gtgactccgg aggaagtgcg cgcgctcgcg gcggaggtgc ttggcaggga
     2341 ccggttcacg ctggcggccg tcggggacct gcccgacgca gggctttcat tctagtgcac
     2401 cgtctcgcaa atatcttggc attcgaccgc cggccgctcc cgggcatcca tgccctccgc
     2461 ggcacttggc catccatggc catcgctgcc ttccccggcg gagttgtgcc ccacatgccg
     2521 atcaaggaac atggtatcgg ctgctggggg taccccaggg agagtatttc gtctgggagt
     2581 ggggcactcc ctcgccgtac ttacaagtac gcctcggtcg tgcgccttgc cgggcacggc
     2641 gcatcgacgt tctcgatgct tcaagctatt tgcgagacgg tacactagcg cgatgggcgc
     2701 ggaatgcccg atccgggtgc ggctgctgcg cgctggaggc gattcgctcc ccgtgtacca
     2761 gacggccggg tcggccggga tggacttgaa agccgacgtc gacggggaga tcgtcatccc
     2821 gccgatgggg a
//
LOCUS       DASVJL010000346         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1089625, whole genome shotgun sequence.
ACCESSION   DASVJL010000346 DASVJL010000000
VERSION     DASVJL010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089625"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /gene="mdh"
                     /locus_tag="VF357_07285"
     CDS             complement(<1..809)
                     /gene="mdh"
                     /locus_tag="VF357_07285"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX7523988.1"
                     /translation="MSRKKITVIGAGNVGATTAQRLAERDFCDVVLVDIVEGMPQGKA
                     LDLMQSGPVYGYDSKVTGTNGYEETAGSDIVVITSGLARRPGMSRDDLLKVNAGIVKD
                     VTLKAIAKSPQAMIIVVSNPLDAMTYVAYKHSKLPTNKVIGMAGILDSARFRAFIAME
                     LGVSVRDVTAFVLGGHGDTMVPSTKYSTVAGIPVEDLIPRDRLAQILERTAKGGAEIV
                     SLLKTGSAYYAPSAAAVQMCDSILLDKKMILPCAVLSSGKYDGCDGLFVGLP"
     gene            complement(1036..2103)
                     /locus_tag="VF357_07290"
     CDS             complement(1036..2103)
                     /locus_tag="VF357_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransacetylase family protein"
                     /protein_id="HEX7523989.1"
                     /translation="MKLYIASTSEYAGKTLLALALGTIWREAGVKVGYVKPLGKIPVM
                     REGRLVDGDATFLADALALDAPPESVCPVVITQDLVMAAWRGEELRLRGKISTAVHDA
                     EARSEVLLVGGTANLRDGIFLGLSPLEFIVNYDCRVVLIDRFEGEKSMDQVLSAAEAL
                     GPRFLGVVFNRVPPAQEDFVRETVIPYFGARGMRVLGVVPSDPVLGSVSVRALAESLS
                     ASVLCGGEDSGKAMIERFCVGAMDVEHALRVFRGIPRKAVVTGGHRTDIQLAALETDT
                     VCLVLTGGVAPNDLILARAREKGVALLAVAEDTMATVDRFESLLGRLRIREPEKIRRG
                     IDLLRSSVDTAGLLSLCRLPS"
     gene            complement(2100..>2601)
                     /locus_tag="VF357_07295"
     CDS             complement(2100..>2601)
                     /locus_tag="VF357_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX7523990.1"
                     /translation="TDVGGVRLGLRGEEDVARAFLEITSSVRRLAPSAWIAGVSIQEM
                     VTGGRELIVGMSRDPQFGPLLMFGLGGIYVEVLKDVSFRVAPVSRRDAEEMVREIRAW
                     PLLAAYRGSEPADEEAIVESLIRVSRLSCDFPEIQELDINPLLVLSKGKGIRAIDCRM
                     TIAEAP"
BASE COUNT          455 a          928 c          794 g          424 t
ORIGIN      
        1 ggcaggccga cgaagagccc gtcgcacccg tcgtactttc cgctcgagag gaccgcgcag
       61 ggaaggatca tcttcttgtc gagcaggatc gagtcgcaca tctggacggc cgccgccgag
      121 ggggcgtagt acgccgaccc cgtcttcagg agggagacga tctccgcgcc gcccttcgcc
      181 gtccgctcga ggatctgcgc cagccggtcc ctggggatga gatcctccac cgggatgccg
      241 gccacggtgg agtacttggt ggaagggacc atcgtgtcgc cgtgaccgcc caggacgaag
      301 gccgtcacgt cgcgcacgga aacgcccagt tccatggcga tgaacgcccg gaaacgggcg
      361 gagtcgagga tccccgccat tccgatcacc ttgttggtcg gaagcttgct gtgcttgtac
      421 gccacgtagg tcatggcgtc gaggggattc gacacgacga tgatcattgc ctggggggac
      481 ttggcgatcg ccttgagggt cacgtccttc acgatccccg cgttgacctt gaggaggtcg
      541 tcgcggctca tccccggcct gcgcgccagc cccgacgtga tgacgacgat gtcggacccg
      601 gccgtctcct cgtacccgtt cgtcccggtg accttgctgt cgtacccgta gaccgggccg
      661 gactgcatca ggtcgagcgc cttcccctgc ggcatcccct cgacgatgtc gacgagcacg
      721 acgtcgcaga agtccctctc ggccagccgc tgcgcggtcg tcgccccgac gtttcccgct
      781 ccgattaccg tgatcttttt ccggctcatt gcgccccccc tacatgcctg cgatgacgcg
      841 aaccacgaac ccgccgcact tcccttcctt ctccgggcta accggcgcct tcgtccattc
      901 cccggacgga atcaaactct tgaagatggc tttttctacc tccatcagcg ggaaaaaaat
      961 atgggatttt ccggatgaga atatggggaa gggtccccca aacctcgcat accgtataca
     1021 aagcccggcg ggatgtcaag agggaagccg gcacagcgac agcagcccgg cggtgtccac
     1081 ggacgagcgc aacaggtcga tgccccggcg gatcttttcc ggttcccgga tccggaggcg
     1141 gccgagaagg ctctcgaacc ggtccaccgt cgccatcgta tcctcggcca cggccaggag
     1201 ggccaccccc ttctcccgcg cacgggccag gatgagatcg ttcggcgcga cgccgccggt
     1261 cagcaccagg cagaccgtgt cggtctcgag cgcggccaac tggatatccg tgcggtgccc
     1321 gccggtgacg accgccttcc gggggatccc ccggaagacc cgaagggcgt gctccacgtc
     1381 catcgcgccc acgcagaagc gctcgatcat cgccttcccg ctgtcttccc cgccgcacaa
     1441 taccgacgcg gagagggact ccgccagggc ccggacgctc acgctcccga gcacggggtc
     1501 cgaaggtacg actcccagca cgcgcattcc ccgcgccccg aagtaaggga tcaccgtttc
     1561 ccggacgaag tcttcctgcg ccggcgggac acgattgaag accacaccga ggaaccgcgg
     1621 cccgagggcc tcggccgccg acaggacctg gtccatcgat ttctccccct cgaaccggtc
     1681 gataagcacg acgcggcagt cgtagttcac gatgaactcc agcggagaga gcccgaggaa
     1741 gatgccgtcc cggaggttcg cggttccccc gacgagcagc acctcggacc gcgcctcggc
     1801 gtcatgcacg gcggtggaga ttttcccacg cagccgcaat tcctcgccgc gccaggcggc
     1861 catcaccagg tcctgggtga ttaccacggg gcaaaccgac tccggcggcg cgtccagggc
     1921 aagcgcgtcc gccaggaacg tcgcgtcccc gtcgacaagc cgtccctccc gcatcaccgg
     1981 gatcttcccg aggggcttga cgtaacccac cttcaccccc gcttccctcc agatcgtccc
     2041 cagggcgagg gcaaggaggg ttttccccgc atactccgac gtcgacgcga tgtagagctt
     2101 catggggcct ccgcgatggt catccggcaa tcgatcgcgc ggatgccttt ccctttcgac
     2161 aggaccagca acgggttgat gtcgagttcc tggatctccg gaaagtcgca cgacaggcgc
     2221 gatacccgga tcagcgactc cacgatcgct tcctcgtccg caggctcgct tccccggtac
     2281 gcggcgagga gcggccaggc gcggatctcc cggaccattt cttcggcatc gcgccgtgaa
     2341 accggggcca cccggaaaga gacgtctttc agtacctcca cgtagatccc gccgagaccg
     2401 aacatcagga gcggaccgaa ctgggggtcg cggctcatcc ccacgatgag ttccctgcct
     2461 cccgtgacca tctcctggat cgacacgccg gcgatccagg ccgatggggc cagccggcgg
     2521 acggaggagg tgatctccag gaaggccctg gccacgtcct cctcgccgcg aaggccgagt
     2581 cgcacgcccc cgacatcggt c
//
LOCUS       DASVJL010000347         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1369844, whole genome shotgun sequence.
ACCESSION   DASVJL010000347 DASVJL010000000
VERSION     DASVJL010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369844"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1987)
                     /locus_tag="VF357_07300"
     CDS             complement(<1..1987)
                     /locus_tag="VF357_07300"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX7523991.1"
                     /translation="MATLTKAARMAWQIGAAEAARLRHPFIEREHLLIGLCSLRKILQ
                     YLDYTHIESLPVEVLREEAGGIERVLAPLGITDAAVRRGVRSRLRPGNAVHPERAVHR
                     SEECKRYFRRAGEIVKDGGEVAVRHLFSAVLEDPGPVVSAVLAGAGISPAGLREKLLV
                     GRELEQEPIPVREEAGDLPVAPEPLSSETPLLDRYGRDITRAAREGRLLPFVESDRTR
                     GTLRQLIKVLMMPTKNNPVLVGETGVGKTAVVEALAERIALGRDRRFLPGRRLVELSM
                     ADMVAGTKYRGEFEERLTRMIEEVRSHPEVILFIDEFHTVAGAGRAEGAPMDAGNIMK
                     PALARGELRCIGATTITEYRHSVEKDPALERRFQPVQVAEPGRDETLEILQGVRAHRE
                     SHFGVTITDETLSATVELAMRFDPTHFLPDKAIDLLDRACAECRVPLLSMAANSDRYD
                     AVGVAVVTPEYVARVVAEKKGIPIEVARGGLGGITESRVRGLEEYLNRYIVGQEEAIS
                     RVCRRLVLSHAGFGDRRRPMGVFLFLGPSGVGKTEVARRIASYLFANERAFIHLDMSE
                     YQEEVSVSRLIGAAPGYVGYEEGGQLIARLRTTPYSVVLLDEVEKASPRVFDLFLQLF
                     DEAKITDAQGHTADARHTIFIMTGNIPVRKEMGFRAGE"
     gene            complement(2015..>2256)
                     /locus_tag="VF357_07305"
     CDS             complement(2015..>2256)
                     /locus_tag="VF357_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7523992.1"
                     /translation="TIFDPTGAGDSFAGGFMGYLASRDGAALTEMDYRLATIYGSAIA
                     SFTVEAFSTERLEGLSRQEIDSRLSSFRALTEFRV"
BASE COUNT          338 a          821 c          736 g          361 t
ORIGIN      
        1 cctcgcccgc gcggaacccc atctccttcc ggacggggat gtttcccgtc atgatgaaga
       61 tcgtgtggcg ggcgtccgcg gtgtgtccct gcgcgtcggt gatcttcgcc tcgtcgaaca
      121 gctgcaggaa caggtcgaac acccgggggg acgccttttc gacctcgtcg agcaggacca
      181 ccgagtatgg ggtggtccgc aaccgggcga tgagctggcc cccctcttcg tagccgacgt
      241 atcccggtgc ggcgccgatg aggcgggaga cgctgacctc ctcctggtac tccgacatgt
      301 ccaggtggat gaacgcgcgc tcgttggcga agaggtacga ggcgatccgc cgcgccacct
      361 cggtcttccc caccccggag gggccgagga acaggaaaac gcccatcggt cggcggcggt
      421 ccccgaaccc ggcgtgggaa aggacgaggc ggcggcaaac gcgggagatc gcctcctcct
      481 gtccgacgat gtaccggttg agatactcct ccaacccccg cacccgggat tcggtgatcc
      541 ccccaagccc gccccgcgcc acctcgatcg ggatcccctt cttctcggcc acgacgcggg
      601 ccacatactc gggcgtgacc acggcgacgc ccacggcgtc gtaccggtcg gagttcgccg
      661 ccatggagag gagcggcacc cggcactccg cgcaggcccg gtccaggaga tcgatcgcct
      721 tgtccgggag gaagtgcgtc gggtcgaatc gcatggcgag ctcgacggtg gcggacagcg
      781 tttcgtccgt gatcgtcacg ccgaagtggc tctcccggtg cgcccggacc ccctgcagga
      841 tctccagcgt ctcgtcccgc ccgggctcgg caacctggac cggctggaag cggcgctcca
      901 gcgccgggtc cttctcgacg ctgtgccggt actccgtgat cgtcgtggcg ccgatgcacc
      961 gcagctcccc tcgggccagc gccggcttca tgatgttccc cgcgtccatc ggggcccctt
     1021 cggcgcgccc cgccccggcc accgtgtgga attcgtcgat gaacaggatc acctccgggt
     1081 gggaccgaac ctcttcgatc atccgggtca ggcgctcctc gaactcgccc cggtacttcg
     1141 tccccgccac catgtccgcc atcgaaagct cgaccagcct ccgtcccggg aggaaccggc
     1201 ggtccctccc gagggcgatc cgttccgcga gcgcctcgac gacggcggtc ttcccgaccc
     1261 ccgtctcccc tacgaggacc gggttgttct tggtgggcat catgaggacc ttgatgagct
     1321 ggcgaagggt gccgcgggtc cggtcggatt cgacgaacgg caggaggcgc ccctctcgcg
     1381 cggcccgggt gatgtcgcgg ccgtaccggt ccaggagcgg cgtctccgag gaaagcggct
     1441 ccggtgcgac gggcagatcc ccggcctcct cccggacggg gatcggttcc tgctccaact
     1501 cccttccaac gagcaacttc tcgcggagac cggccgggga aatccccgcg cccgcaagga
     1561 ccgcggagac gaccggaccc gggtcctcga gaacggcgga aaaaaggtgg cgcacggcga
     1621 cctccccccc gtccttcacg atctcccccg cccggcggaa gtaccgcttg cactcctcgc
     1681 tgcggtggac cgctcgctcc ggatggaccg cgttccctgg ccgcaggcgc gaccgcacac
     1741 cgcggcgcac tgctgcatcg gtgatcccga ggggcgcgag aacccgttcg atcccgcccg
     1801 cctcctcccg aaggacttcg accgggagcg actcgatgtg ggtgtagtcc aggtattgca
     1861 ggattttccg gaggctgcac agcccgatca ggaggtgctc ccgctcgatg aacgggtgcc
     1921 ggagccgtgc cgcctcggcc gcgccgatct gccacgccat tctggcggct ttggtcaacg
     1981 tcgccatggt cgatctcccc ccgttcctcc ccgttcagac ccggaattcg gtgagcgcac
     2041 ggaacgacga gaggcgggag tcgatctctt gccgggaaag cccctccagc cgctccgtgc
     2101 tgaacgcctc caccgtgaag gaggcgatgg cgctgccgta gatcgtggcc agccggtaat
     2161 ccatctccgt gagcgcggcc ccgtctcgcg aagcgaggta tcccatgaag ccgccggcga
     2221 agctgtctcc cgccccggtg gggtcgaaga tcgtct
//
LOCUS       DASVJL010000348         5121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1651464, whole genome shotgun sequence.
ACCESSION   DASVJL010000348 DASVJL010000000
VERSION     DASVJL010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651464"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VF357_07310"
     CDS             <1..386
                     /locus_tag="VF357_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523993.1"
                     /translation="SDRLANVGVTNRGALRERAGDIAEKGISVSTFVVGNAFDEELLT
                     MVAGGGGGNYRYLGDPERTVAALESEFHTVSRTAASEVEIILRLKRECPSARSSGATG
                     GATATPTSSGWAIFPRASVGPSSPS"
     gene            275..823
                     /locus_tag="VF357_07315"
     CDS             275..823
                     /locus_tag="VF357_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523994.1"
                     /translation="MPFGSVLGRDWRRDGDAYVIRLGDLSAGERRTVFAKLNVAGEWA
                     GLREVGEVALRYRDPVTSKVVTASAKGVSLELVRDERVYREGFDRSVQEKRAVAEANL
                     LVQEAARLADQGKKEEAKGMLGKAAAGLAAAPPSPAVRAEMDHAERYKNSLDTMGDMS
                     SESAKGAQKAIKYRAYETLQQR"
     gene            1133..1543
                     /locus_tag="VF357_07320"
     CDS             1133..1543
                     /locus_tag="VF357_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523995.1"
                     /translation="MEFLKYDARRFFLFGIRFFFGAWLLYVGLSKWIFMGPGVFVGYI
                     TSQFEKTWSPHVLNVFLAWLIMIAEPVLALLILSGKKARKVWTLASLFMFLLTIGQTI
                     LMKPDVIANWQLLMLVLLCAALSDPDPRNGDAAK"
     gene            complement(1591..2394)
                     /locus_tag="VF357_07325"
     CDS             complement(1591..2394)
                     /locus_tag="VF357_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7523996.1"
                     /translation="MITFLVFLVFGIIVSGLYEKRHAAEEKLTILTAELQRSNRDLEQ
                     FAAIASHDLKEPLVTVGGFLALLKRRNEGDLDAEANRHIRHALESVERMERLIGDLLE
                     YSRVGTHASLFQVIDSSAILEIALKNLAGTLHSKGALVTHDPLPEIIADSTQIGQLFQ
                     NLIGNAIKFCRCDTPRIHVSAVREEREHVFSVQDNGIGIDPESTDEIFTAFRRLHDRD
                     EFPGTGLGLATCAKIVERHSGRIWVESNVRQGSTFYFTIAVREPDSTGP"
     gene            complement(2754..3134)
                     /locus_tag="VF357_07330"
     CDS             complement(2754..3134)
                     /locus_tag="VF357_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523997.1"
                     /translation="MKRWKSIVGVLLVFLLGALAGAAVEHRVVRQSFENVLSGRGGAT
                     VDLIVRRLSRSLDLDTAQQDQVRAIVTETHRNIGEIRKPVQAQVEAAIDRSRVRVRAI
                     LRPDQQEKFDRIQAERRKRWGRQE"
     gene            complement(3140..3457)
                     /locus_tag="VF357_07335"
     CDS             complement(3140..3457)
                     /locus_tag="VF357_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7523998.1"
                     /translation="MKDKRHDRRDVELDRFFALARQASPDISGVERNFETRVIARIRE
                     GRERQEPWYAWAWRLAPVFLALTLLLAGWSLFYSPVRPADPVATLASGTQEVALLNNL
                     TGD"
     gene            complement(3447..4022)
                     /locus_tag="VF357_07340"
     CDS             complement(3447..4022)
                     /locus_tag="VF357_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7523999.1"
                     /translation="MYALGVPMAVPDKDADLVRSAIGGDEEAFTELVRRHKGTVLRSA
                     ARFARNHAELEDLGQETFLRVYRDLRSFRGDAPFAHWLSRITVHVCYDALRKRRREEK
                     DVSLEVLAAPVADPSTGNGPSAQLARMILGQAMSRLKPEDRLVITLFELEDRSVREVA
                     ELTGWSETRVKVRAFRARQALKRHIGGDDER"
     gene            complement(4026..4553)
                     /locus_tag="VF357_07345"
     CDS             complement(4026..4553)
                     /locus_tag="VF357_07345"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX7524000.1"
                     /translation="MKTLHKGFTALVLLTAVAVGAAIVSGNAWADRGGDGGGRCACSG
                     RDGGWKGHCHRGHRLAKALGLSKEQQEQVTAIFRKHRDEFAPLRKEMVSERRELRNLI
                     QSDKPDEAAIREQAKKIAATSGDLAVRRAKMSQEVRTVLTPDQIQKFRALQEKRDRRI
                     DRMMNRGQEGQSKGE"
     gene            complement(4623..>5121)
                     /locus_tag="VF357_07350"
     CDS             complement(4623..>5121)
                     /locus_tag="VF357_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7524001.1"
                     /translation="AEAAFYPNITLSASAGLEAASLAKWLTWPARFWSVGPAVSEVLF
                     DGGLRRAQTDQARAAYDAEVSAYRQTVLTGFQEVEDSLAALRVLEEEAQVQQEALAAA
                     RQTVDVVTNQYKSGMVSYLNVIVVQVIALSNEQAALNILGRRMAASVSLVKALGGGWD
                     PSALR"
BASE COUNT          916 a         1623 c         1575 g         1007 t
ORIGIN      
        1 tctcggacag gctggccaac gtcggcgtga ccaatcgcgg cgccttgcgg gagcgggcgg
       61 gggacattgc ggagaagggg atctccgtct cgacgttcgt agtcggcaac gctttcgacg
      121 aggagttgct gacgatggtc gccggaggcg gcggcgggaa ttaccgctac ctcggcgatc
      181 ccgagcggac cgtggcggcg ctggaatccg agttccacac cgtctcgcgg acggccgctt
      241 ccgaggtcga gatcatcctc cggctcaaga gagagtgccc ttcggctcgg tcctcgggcg
      301 cgactggcgg cgcgacggcg acgcctacgt catccggctg ggcgatcttt ccgcgggcga
      361 gcgtcggacc gtcttcgcca agctgaacgt ggccggggaa tgggccgggc tgcgggaagt
      421 cggagaggtg gcgctccggt atcgcgatcc ggtgacatcg aaggtggtca ccgcttccgc
      481 gaagggagtt tccctcgagc tcgtccgcga cgagcgggtc tatcgggagg ggttcgaccg
      541 gtcggtgcag gaaaagaggg ccgtcgcgga ggcgaatttg ctggtgcagg aagcggcccg
      601 gctggccgac caggggaaga aggaggaggc gaaggggatg ctcggaaagg ccgcggcagg
      661 gttggccgcc gcccctccgt cgcccgcggt ccgcgcggag atggatcatg cggaacggta
      721 caagaacagc ctcgacacga tgggcgacat gagctcggag tcggccaagg gggcccagaa
      781 ggcgatcaag taccgcgcgt acgagacgct ccagcaacgg tagggaatcg gtggaaggcg
      841 gggaggggac gatgtcccct ccacgccttc ccgcggaatg aatgggctat aacgtcactt
      901 tttcccgcat ccacaggtgt tgtacatgtc gccaccccct ttgttttcca ttagatgccg
      961 ggttgcgatg aaaggtgacg atctccgatg cggcatccga tccgctcttt ccggaatcat
     1021 acggggagaa gtttttttta tcaggtcaag cgccggtcct tgcgaatccg ggggatcccg
     1081 tggggttgtt tcccggccgg aatccgatcc gactcgcacg ggagggggga gtatggaatt
     1141 tctgaaatac gacgcccgta gattttttct gttcggcatc cgattctttt tcggggcgtg
     1201 gttgctgtac gtggggctct ccaaatggat cttcatgggg cctggcgtgt tcgtcggcta
     1261 cattacctcc cagttcgaga agacctggtc tccacacgtg ctgaatgttt tcctcgcctg
     1321 gctgataatg atcgcggagc cggtgttggc cctgctgatt ctttccggga agaaagcgcg
     1381 caaggtatgg acgctggctt ctttgttcat gtttctgctg accatcggcc agaccatcct
     1441 catgaagccc gatgtcatcg ccaactggca gttacttatg ctggtcctgc tatgcgccgc
     1501 cctcagtgat ccggatcccc ggaatggtga cgcggcgaag taaatgaatt cctatcgccc
     1561 gaggcacggt caccccttcc accaaccacg tcagggtcca gtcgagtcgg gttcccttac
     1621 cgcaattgtg aagtagaagg tggatccctg ccgaacgttt gattccaccc agattcggcc
     1681 gctgtgtcgt tccacgatct ttgcgcaagt cgcgaggccg agacctgtgc cggggaactc
     1741 gtctctgtca tgcagccgcc ggaaggccgt gaagatctcg tccgtggatt cgggatcgat
     1801 cccgatgcca ttgtcctgga cggagaagac atgttccctc tcctcacgga cggcggaaac
     1861 gtggatgcga ggtgtatcac atcgacagaa cttgatcgcg ttgccgatga ggttctggaa
     1921 gagttggccg atctgggtag agtccgcaat gatttcaggg agcggatcat gggtcaccag
     1981 ggcccccttc gaatggagcg ttcccgcgag attcttcagc gcgatctcca ggatggcgga
     2041 agagtcgatc acctgaaaca gactggcgtg ggtaccgacg cgggaatatt ccagaagatc
     2101 cccgataagt cgctccatcc tttccacact ttccaaggcg tggcggatgt ggcggtttgc
     2161 ttcggcatcc aggtccccct cgttccgccg cttgaggagg gcaaggaacc ctccgacggt
     2221 aaccaatggt tccttcaagt catgggacgc aatggctgcg aactgttcca gatcccggtt
     2281 gctccgctgc aactccgccg tgaggattgt cagtttctcc tccgccgcat gtcgcttttc
     2341 gtataacccg gatacgatga tgccgaacac gaggaatacc aggaaggtga tcacccggtt
     2401 ataaaattca tcggcgggaa tgtggaccag atggtcgaaa aaagtcctgc cggatccgta
     2461 ataaaaaaca aaccgctcaa ataatgcgtc gaggatccag gataatattc ctgcggcgac
     2521 cgatatgaga agcaggcctt tgcctgattt caaaatcgtt cccccggagt tccggccgca
     2581 acgatctttc caataaaatt attctactcc cgtgacgaga aggttctgca tgtcccttcc
     2641 gcatcgagcc gtacgattcc atgtcgtgcg ctccggggaa aaatcggaag gtgtgccatc
     2701 aatggtcaac ccctcctcat gcggggtggg ccccgtttgt ttcgaagacg ggactactcc
     2761 tgccgccccc atcgcttccg gcgctcggcc tggatgcgat cgaacttctc ctgctggtcg
     2821 gggcgcagga ttgcacgaac gcgaacgcgg gagcgatcga tcgccgcctc gacctgggcc
     2881 tggaccggct tccggatctc gccgatgttc cggtgcgtct cggtgacaat ggcgcgaacc
     2941 tggtcctgct gggcggtgtc gaggtccagc gaccgggaaa gccgcctgac gatgaggtcc
     3001 accgtggcgc cacctcttcc ggagagaacg ttttcgaagc tttggcggac gacgcggtgc
     3061 tcgaccgccg cgcccgcaag agcgccgagc aggaagacca gcaggacgcc gacgatcgat
     3121 ttccatcgtt tcatgttcgt caatctcccg tcagattgtt gagcagcgcg acttcctggg
     3181 ttcccgaggc gagcgtcgcc accggatcgg ccggacgcac cggcgaatag aataagctcc
     3241 accccgcgag caggagggtc agcgccagga agaccggagc cagccgccac gcccaggcat
     3301 accagggctc ctgccgttca cgcccctccc ggatcctcgc gatcacgcgc gtctcgaagt
     3361 ttcgttcgac cccggagatg tccggggatg cctgccgcgc cagtgcgaaa aaccggtcga
     3421 gctccacgtc cctccggtcg tgtcgtttat ctttcatcgt cccctccaat atgccgtttc
     3481 aatgcctgcc gcgcccggaa agcgcgcacc ttcacccgcg tttcgctcca ccctgtcaac
     3541 tccgccactt cccggaccga ccggtcctcc agttcgaaca gcgtgatcac cagccggtcc
     3601 tcgggtttga ggcgtgacat cgcctggccg aggatcatgc gggccaactg ggcggaaggg
     3661 ccgtttcccg tggaagggtc cgcgaccggt gcggccagga cctccagcga aacgtccttc
     3721 tcctcccgtc gccgtttccg cagcgcgtcg taacatacgt gcaccgtgat ccgcgagagc
     3781 cagtgcgcga aaggggcgtc gccgcgaaag gagcggaggt cccggtagac cctcaggaac
     3841 gtctcctgtc cgagatcctc cagctccgca tgattacgcg cgaagcgggc ggcgctgcga
     3901 aggaccgtac ccttatgtcg gcgcaccagc tccgtaaacg cctcctcgtc gcctccgatc
     3961 gcggagcgga cgagatcagc atccttgtcc ggcaccgcca tcggcacccc caaagcgtac
     4021 aagcactatt ctcccttcga ctgcccttcc tgcccgcggt tcatcatccg gtcgatccgc
     4081 cgatcccgct tttcctgcaa ggcccggaac ttctggatct gatcgggtgt cagcaccgtc
     4141 cgcacttcct gggacatctt ggcccggcga acggcgagat ccccgctcgt cgcggcaatc
     4201 ttcttcgcct gctcccggat ggccgcctcg tccggcttgt ccgactggat caggttccgc
     4261 aattcccgcc gctcggaaac catttccttc cgaagcggag cgaattcgtc acgatgtttc
     4321 cggaagatgg ccgtgacctg ctcctgttgc tccttggaca ggccaagcgc cttcgccagc
     4381 cggtggcccc ggtggcagtg tcccttccac ccgccatccc gtccggagca ggcgcaccgt
     4441 ccaccaccgt ctccgccgcg atcggcccag gcgtttccgg aaacgatcgc cgctcccacg
     4501 gcaaccgccg taaggaggac cagtgcggta aatcccttgt gcaatgtttt catcgttccc
     4561 tccctgatcg gccgtttcga tcggcctctt tccctttaat ggcgtcaggg cgatgggaaa
     4621 ggttaccgca gggccgacgg gtcccagccg cctcccagcg ccttgacgag cgacacgctg
     4681 gcggccatcc ggcgacccag gatgttgagc gctgcctgct cattggaaag ggcgatgacc
     4741 tggacgacga tgacgttcag gtagctgacc atcccggatt tgtactggtt ggtgacgaca
     4801 tccaccgttt gtcgcgcggc cgcaagcgcc tcctgttgca cctgcgcctc ctcctccaga
     4861 acgcggaggg cggcgaggct gtcctccacc tcctggaacc ccgtcagcac cgtctgccgg
     4921 tacgccgaaa cctccgcgtc gtaggccgcc cgggcctggt cggtctgcgc ccggcgaagg
     4981 cctccgtcga acaggacctc cgagacggcc gggccgaccg accagaagcg ggcgggccag
     5041 gtcagccatt tcgccaggct ggccgcctca agccccgccg acgcgctcaa tgtgatgttc
     5101 ggatagaagg cggcctcggc g
//
LOCUS       DASVJL010000349         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1276894, whole genome shotgun sequence.
ACCESSION   DASVJL010000349 DASVJL010000000
VERSION     DASVJL010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276894"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VF357_07355"
     CDS             <1..557
                     /locus_tag="VF357_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HEX7524002.1"
                     /translation="FAPIVKFLISCASSLTVPFLVLVYAVVFFIVIPLAAGVASRTLL
                     IRAKGSEWYEKFLAVLHPVTIVALLATLVCIFAFQADNITARSFHILLIAVPILIQVY
                     FNSSLAYGLMKWLNVPHNVAAPGALIGASNFFELAVATAIALYGPGSGAALATVVGVL
                     VEVPVMLSVCSFCNRTRHWFPAEI"
     gene            597..>2256
                     /locus_tag="VF357_07360"
     CDS             597..>2256
                     /locus_tag="VF357_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524003.1"
                     /translation="MAFETRAMDPKATNHRWRFFRAGGFDQVLLETGADLMSLEALDQ
                     KLWVALSCPVSGIEFDAKSLAFVDADADGHIRAPEILAAVQWAGKVLKDPEYLIHRKD
                     RLPLSAIDEGTEEGKAVLGGAKLVLSVLGESKATEITLDDASDTARIFSQARFNGDGI
                     VPAKSAEDPALAAVIEEILGSLGGENDRGGEPGVTAEAIERFYTEAKAIADWWEAVGK
                     PETHPFGADTAARYELFLSLKPKIEDYFQRCRLAAYDGRSVALLGPAEADYQNLAATT
                     LRGDTEAVAGFPLSVAAAGKALSLADALNPVYADAVRRFAVEIVLPVLGSAESLTDGG
                     WGTICARFAPYEAWLEAKPATAVEKLGEVRVRAILAEDSKAALLDLVTRDKAVEPEVT
                     AIVSVERLLRYCRDLHLLVNNFVSFRDFYTRKGKATFQAGTLYLDGRSCDLCITISDV
                     GKHAAIATLSRVCLAYCDCVRRGGGEKTTIAAAFTAGDSDFLTVGRNGVFYDRRGQDW
                     DATIVRILEHPISVRQAFWAPYKRLARLIGEQTQKMAAARSKVAD"
BASE COUNT          371 a          699 c          784 g          402 t
ORIGIN      
        1 tgttcgcccc gatcgtgaag ttcctgatca gctgcgcgtc ctcgctgacg gtgccgttct
       61 tggtgctcgt ctacgcggtg gttttcttca tcgtcatccc tctggccgcc ggggtcgcat
      121 ccaggacctt gctgatccgt gccaagggga gcgaatggta cgagaagttc ctggcggtcc
      181 tccaccccgt gacgatcgtc gcgctgctgg ccactctggt gtgcatcttc gcgttccagg
      241 ccgacaacat caccgcccgg tcgttccaca tcctgctgat cgccgtcccg atcctcatcc
      301 aggtctactt caactcgtcg cttgcctacg gattgatgaa gtggctgaac gttccccaca
      361 atgtggcggc acccggggct ttgatcgggg cgagtaattt cttcgaactg gccgtggcca
      421 ccgcgatcgc cctctacggc cccggatccg gcgcggcgct ggccacggtg gtcggggtgc
      481 tggtggaagt tcccgtcatg ctctccgtct gctcgttctg caaccggacc cgccactggt
      541 tccccgccga aatctgatat ccttccttgt atacaggaga acaggaggac gaatccgtgg
      601 catttgaaac cagggcgatg gatccgaaag ccacgaatca ccggtggcgg ttctttcgcg
      661 cgggcggatt cgaccaggtc ctcctcgaga cgggcgccga cctcatgtcg ctcgaggcgc
      721 tggaccagaa actctgggtg gccctctcct gcccggtgtc cgggatcgag ttcgacgcga
      781 agagcctcgc gtttgtcgac gcggacgcgg acggccacat ccgggcgccc gagattctcg
      841 ccgccgtcca gtgggcgggg aaggtcctga aggacccgga atatctcata catcggaagg
      901 atcgcctgcc gctgtcggcc atcgacgaag gaacggagga ggggaaggcg gttctggggg
      961 gcgcgaaact ggtgctctcg gtcctcgggg agtcgaaggc gacggagatc accctcgacg
     1021 acgccagcga cacggcgcgg atcttctccc aggcgcggtt caacggagac gggatcgtgc
     1081 cggcgaagtc ggcggaagac ccggcgctgg cggcggtgat cgaggagatc ctcgggtccc
     1141 tgggcgggga aaacgaccgc ggcggcgagc cgggggtgac ggccgaggcg atcgaacggt
     1201 tctatacgga ggcgaaggca atcgcggact ggtgggaggc ggtcgggaag ccggagaccc
     1261 atccgttcgg cgccgacacg gcggcccggt acgaactttt cctctccctg aagcccaaga
     1321 tcgaggatta tttccagcga tgccggcttg ccgcatacga cggacgatcg gtggcgctgc
     1381 ttggtcccgc ggaagcggac tatcagaatc tggccgcgac gacgttgcgg ggggacacgg
     1441 aggcggtggc cgggtttccg ctctcggtgg cggcggcagg gaaagcgttg tccctggcgg
     1501 acgctttgaa ccccgtgtac gccgatgcgg tgcggagatt cgcggtggag atcgtcctgc
     1561 cggtccttgg aagcgctgaa tccctgacgg acggcgggtg ggggacgatc tgcgcgcgct
     1621 tcgctccgta cgaagcgtgg ctggaagcga aaccggcgac ggcggtcgag aaactcggcg
     1681 aggtgcgggt gcgggcgatc cttgcggagg acagcaaggc ggcgctgctc gatctcgtca
     1741 cgcgggacaa ggcggtggag ccggaggtga ccgccatcgt ctcggtggag cggctcctcc
     1801 ggtactgccg cgacctccac ctcctggtga acaacttcgt ctccttccgc gacttctata
     1861 cgcgcaaggg gaaagcgacc ttccaggcgg gcaccctgta cctcgacggc cgcagctgcg
     1921 acctttgcat tacgatctcg gatgtcggaa aacatgcggc gatcgcgacg ctctcccgcg
     1981 tctgcctggc ctactgcgac tgcgtgcgtc gggggggcgg ggagaagacg acgatcgcag
     2041 ccgccttcac ggcgggcgat tccgacttcc tcacggtcgg cagaaacggg gtcttctacg
     2101 accggagggg gcaggactgg gacgccacga tcgtgcggat cctcgagcac ccgatcagcg
     2161 tccggcaggc gttctgggct ccgtacaagc ggcttgcccg cctgatcggg gagcagacgc
     2221 agaagatggc cgcggcacgc tccaaggtag cggacg
//
LOCUS       DASVJL010000350         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_619462, whole genome shotgun sequence.
ACCESSION   DASVJL010000350 DASVJL010000000
VERSION     DASVJL010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619462"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VF357_07365"
     CDS             <1..1159
                     /locus_tag="VF357_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX7524004.1"
                     /translation="VFEAMRPYFLEWYGNPSSHIHDQGQAALRAVDQARAEVAALIGA
                     KAGEIVFTSGATESNNLAVLGAAEGAGAGKRHVVVSEVEHFSVINALVPLRNAGYEVT
                     ILPVDRNGVVDPDAVRKALRADTALLSVMHANSEIGTIEPVAPIGRIAKEAGVTFHVD
                     ATASTGHIPLDVREIPADLVTLSAHNFYGPKGAGALYLREGTKVAPRTFGGFQEMGYR
                     AGTENVPAVVGMGAAAILAGREMGAWAEHLSRLGKTLWDGLAAIPMLHYTGHPSERLP
                     GHVSFWVEHVEGESLLLFLNMKGVMAASGSACSSNLRGHDEEDLVASPVLRAIGVPSD
                     ICTGSITFSLGKGNTADEVRSALDALPGIVDRLRAMSPTYLDYQKQQVKGG"
     gene            1167..1550
                     /gene="nifU"
                     /locus_tag="VF357_07370"
     CDS             1167..1550
                     /gene="nifU"
                     /locus_tag="VF357_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HEX7524005.1"
                     /translation="MAAGPYSEKVMDHFMNPRNVGEIDGADGVGEVGNPACGDMMRLY
                     LKIEEGRVQDAKFRTFGCGAAIASSSMLTEMIKGKTVDEARAITNLQVAEALDGLPAV
                     KIHCSVMAEQAVKSALDDYVKKHAG"
     gene            1577..1774
                     /locus_tag="VF357_07375"
     CDS             1577..1774
                     /locus_tag="VF357_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX7524006.1"
                     /translation="MPVNPMDVIRMALDREKTAYRNYTEYARIATQPEIRELFQYLAE
                     EEKKHVKLLSDEIEKETFQEM"
     gene            1779..>2058
                     /locus_tag="VF357_07380"
     CDS             1779..>2058
                     /locus_tag="VF357_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524007.1"
                     /translation="MPPCAGMRLILYDSASRGNVPFAPSDPERVRIYTCGPTVYRYAH
                     IGNLRTYLLTDLLVRTLRFLGYGTFTVQNITDMGHMHQEQLEAGEDKVI"
BASE COUNT          378 a          655 c          708 g          317 t
ORIGIN      
        1 cgtcttcgag gcgatgcgtc cgtacttcct cgaatggtac ggcaacccgt cctcccacat
       61 ccacgaccag gggcaggcgg cgttgcgggc ggtcgaccag gcgagggccg aggtcgcggc
      121 gctgatcggg gcgaaggcgg gagagatcgt cttcacctcg ggggccaccg agtcgaacaa
      181 cctcgccgtc ctcggcgcgg cggagggcgc gggcgccggg aagcggcacg tcgtcgtgtc
      241 ggaagtcgag cacttctccg tgataaacgc cttggtcccg ctgcgcaacg cggggtacga
      301 ggtgaccatt cttcccgtgg accggaacgg cgtggtcgac cccgacgccg tccggaaggc
      361 gcttcgggcc gacacggcgc tcctctcggt gatgcacgcc aactccgaga tcggcacgat
      421 cgagccggtc gcgccgatcg ggcggatcgc gaaggaggcg ggcgtcacgt tccatgtcga
      481 cgccaccgcg tcgacgggac atatcccctt ggatgtccgt gagatccccg cggatctcgt
      541 cactctctcg gcgcacaatt tttacggacc gaaaggggcc ggggcgctgt acctccgcga
      601 ggggacgaag gtggcgccgc ggacgttcgg cgggttccag gagatggggt accgcgccgg
      661 cacggaaaac gttcccgccg tggtggggat gggcgcggcg gcgatcctcg cgggtcggga
      721 gatgggggcg tgggcggagc atctctcccg cctggggaag acgctgtggg acggcctggc
      781 cgcgatcccc atgctccact acacggggca cccttccgaa cgtctccccg ggcacgtctc
      841 gttctgggtg gagcatgtcg agggggagtc gctcctcctg ttcctcaaca tgaagggggt
      901 catggccgca tccggctccg cgtgcagctc caacctgcgg ggacacgacg aggaggatct
      961 cgtggcctcc cccgtcctcc gggcgatcgg cgtgccgagc gacatctgca ccgggtcgat
     1021 caccttcagc ctagggaagg ggaacaccgc agacgaggtc cgttcggcac tcgacgcgct
     1081 ccccggcatc gtggaccggc tcagggcgat gtccccgacg tatctcgact accagaagca
     1141 gcaagtcaaa ggaggatgac cggatcatgg cggcgggacc ctatagcgaa aaggtgatgg
     1201 accacttcat gaacccccgg aacgtggggg agatcgacgg ggcggacggc gtcggcgagg
     1261 tcggaaaccc cgcctgcggc gacatgatgc gactctacct caagatcgag gaaggacggg
     1321 tgcaggacgc gaagttccgc acgttcgggt gcggggcggc gattgcctcc agttcgatgc
     1381 tcacggagat gatcaagggg aagaccgtgg acgaggcccg cgcgatcacg aacctgcagg
     1441 tggcggaagc cctcgacggg ctcccggcgg tcaagatcca ttgctccgtg atggcggagc
     1501 aggccgtgaa atccgccctc gacgattacg tcaagaagca cgctggctga gaagactcct
     1561 cctggaggat atcgagatgc ccgtcaaccc gatggatgtg atcaggatgg ccctggaccg
     1621 cgagaagacc gcgtaccgga actacacgga gtacgcccgg atcgccacgc agccggagat
     1681 ccgggagttg ttccagtatc tcgcggaaga ggagaagaag cacgtgaagc tcctgtccga
     1741 cgagatcgag aaggaaacgt tccaggagat gtaggacggt gcccccgtgc gccgggatgc
     1801 gtctcatcct ttacgacagc gcatcccggg ggaacgtccc cttcgcaccc tccgacccgg
     1861 agcgggtccg catctacacg tgcggcccca ccgtctaccg ctacgcccac atcggtaacc
     1921 ttcgcaccta tctgctgacc gacctcctcg tccgcaccct tcgcttcctg gggtacggca
     1981 cgttcaccgt ccagaacatc acggacatgg ggcacatgca ccaggagcaa ctggaggcgg
     2041 gcgaggacaa ggtgatcg
//
LOCUS       DASVJL010000351         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1652134, whole genome shotgun sequence.
ACCESSION   DASVJL010000351 DASVJL010000000
VERSION     DASVJL010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652134"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VF357_07385"
     CDS             <1..792
                     /locus_tag="VF357_07385"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7524008.1"
                     /translation="GLRVQGVPLGGRNRKTSEMVELVGLAEEADRPVETLSGGQRQRV
                     ALARALAVSPSLLLLDEPLLALDIKVRKRLRREIAAVQKKVGITTLIVTHDQEEAMEV
                     GDRIAVMNDGRIEQVASPREVYQEPATEFVAKFIGEVNVLPGQLYRGSAYAGNLAIRM
                     EGDGVPFHGGTVKVLLRPEDVTLLPPGIAESGMTHANVKSVSWLGAHLRMEIATEEGY
                     LLTALLQRSHPMAGRVAAGDTVRVAALRGTVLPDPLGAKEPEYFL"
     gene            800..2089
                     /gene="cobA"
                     /locus_tag="VF357_07390"
     CDS             800..2089
                     /gene="cobA"
                     /locus_tag="VF357_07390"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX7524009.1"
                     /translation="MSAMQRYLPVFHDVRGARVLVIGAGAVGARKIEALLAGGARVTV
                     VAKEFSPAVKERAARGDLTVLRGTFHPDQMREAEFVFVATPDRALNRRVSAEARRRRV
                     PVNVADSPEECTFLLPAVVRGEEFTTAISTSGRHPGAAKALREFLEDHREEISVRLER
                     GRRRKRIAARPGKVYIVGAGPGDPDLLTVRALGLLRSADAVIYDYLVPREILALAPAK
                     ASRICFARRGRTAGHGAFLKQNAIQGAMVRLAREGKSVVRLKSGDPLLFGRGGEEAEH
                     LSREGIPFEIVPGITAAMGCAAAAVLPLTHRERSSSVTFVAGHGADEKGGSAVEWSHL
                     PKDGTLAIYMGVGRIAAIAEELAGAGFSPNTPFAIVENGSRPDQRVVRGILSGLSRIA
                     LDSNIHSPAILFVGRTAAFLPFENTEETSDEPVAQGV"
     gene            2096..2971
                     /locus_tag="VF357_07395"
     CDS             2096..2971
                     /locus_tag="VF357_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7524010.1"
                     /translation="MNGPDDKELKKGGMMRQAEAGLFSVRLHVVGGRLATPQLRAIQE
                     AADRFGRGEIHLTSRQGVEIPHIPQGALAALKEFLAPSGVGEGVAGPTVRTVTACQGC
                     RVCPRGVIDSPALAEAVDRELYGKPVPHKFKVGISGCVNNCMKAEENDVGIKGWIEPR
                     WEVPACTFCGICQTVCPTKVISMSGDGHALIVDSTLCIGCGDCITSCPTGSMREQIRG
                     YRIFAGGKFGRRPSLGKRILGVLRRKEDAMKAIPAVLDFFREHGKPRERFGDTLQRTG
                     LPALVTFVNERTGLS"
     gene            2991..>3631
                     /locus_tag="VF357_07400"
     CDS             2991..>3631
                     /locus_tag="VF357_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7524011.1"
                     /translation="MKKNRSVETIAAQAGVGADKAFGAVSVPIYLSAIYRYERFGKNR
                     GFDYSRGENPTRKAVEKALADLEGGSRAVAFSSGMAAISALMTLFRTGDHILCSDDLY
                     GGTYRLFEKLLRPYGLSFDYVDMGNPAAVRKKIRGKTKALFIETPTNPLMKIADLKGA
                     ARIGREKGVLTVVDNTFMSPLLQRPLGLGIDVVVHSATKFLGGHNDLIGGAVVT"
BASE COUNT          625 a         1146 c         1296 g          564 t
ORIGIN      
        1 ggcctccgcg ttcagggggt gccgctcgga ggccggaatc ggaagacgtc ggagatggtc
       61 gaactcgtgg gcctcgcgga agaggcggac cgcccggtgg agaccctctc gggggggcag
      121 cggcaacgcg tcgccctcgc ccgggcgctc gccgtctccc cttccctgct gctgctcgat
      181 gaaccgcttt tggcgctgga catcaaggtc cggaaacggc tgcgccgcga gatcgccgcg
      241 gtgcagaaaa aagtagggat caccacgctg atcgtcacgc acgaccagga ggaggcgatg
      301 gaggtggggg accggatcgc ggtgatgaac gacggaagga tcgaacaggt cgcctccccg
      361 cgggaggtgt accaggagcc cgccaccgaa ttcgtagcga agttcatcgg agaggtcaac
      421 gtcctcccgg gccagttgta ccggggatcc gcctacgcgg gaaacctggc gatccggatg
      481 gagggggacg gggtcccctt ccatggagga acggtcaagg tcctgttacg gcccgaagac
      541 gtgacgctcc ttccgccggg gatcgcggag tccgggatga cccacgcaaa cgtgaaatcg
      601 gtttcctggc tcggagcgca cctccggatg gagatcgcca cggaggaggg gtacctcctc
      661 accgccctgc tgcagcgcag ccaccccatg gcgggtcgcg tggcggccgg cgacacggtc
      721 cgcgtggccg ccttgcgggg aaccgttctc cccgaccccc tcggggccaa agagccggaa
      781 tatttcctgt gaggttccca tgagcgcaat gcagcgatac ctgccggtct tccacgatgt
      841 tcgtggcgca cgtgtcctcg tgatcggcgc cggcgcggtg ggtgcacgga agatcgaagc
      901 gcttctcgcg ggcggggcgc gggtcacggt ggttgcgaag gagttttccc cggccgtgaa
      961 ggagcgcgcg gcgcgcgggg atctcacggt cctccggggc acgttccacc ccgaccagat
     1021 gcgggaggcc gaattcgtct tcgtggccac ccccgaccgg gcgctgaacc gccgcgtatc
     1081 ggccgaggcc cggcggcgcc gtgtacccgt caacgtggcg gactctccgg aggagtgcac
     1141 cttcctcctc cctgccgtgg tccggggaga ggaattcacg accgcgatct ccacgagcgg
     1201 gaggcacccg ggggcggcga aagcgttgcg ggagttcctc gaggatcacc gggaggagat
     1261 ctcggtccgc ctggagcggg ggcgacgccg gaagcggatc gcggcgagac cgggaaaggt
     1321 ctacatcgtc ggcgccgggc cgggcgaccc cgacctgctc accgtgcgcg cgctgggact
     1381 gctgcgctcc gcggacgccg tcatttacga ctacctggtc ccgcgggaga tcctcgcgct
     1441 ggcgcccgca aaggcatccc ggatctgctt cgcccggcgg ggaagaaccg ccggccacgg
     1501 ggcgttcctg aagcagaacg cgatccaagg ggcgatggtc cggctggcgc gggaggggaa
     1561 atccgtcgtc cgcctgaaat ccggcgaccc gctcctattc ggtcggggcg gcgaggaggc
     1621 ggagcatctc tcgcgggaag ggattccttt cgagatcgtc ccggggatca ctgccgcgat
     1681 ggggtgcgcg gcggcggcgg tccttccgtt gacgcaccgc gagagatcct cttccgtcac
     1741 cttcgtcgcc ggccacgggg ccgacgaaaa ggggggaagc gccgtcgaat ggagccatct
     1801 gccgaaagac gggaccctcg ccatctacat gggagtcgga cggatcgcgg ccatcgcgga
     1861 agaactcgcc ggagcggggt tttctcccaa taccccgttc gccatcgtgg agaacgggag
     1921 ccggccggat cagcgcgtcg tccgcggcat cctatccggc ctctcccgga tcgcccttga
     1981 ttccaatata cactcccctg cgatcctctt cgtggggcgc accgccgcgt tcttgccatt
     2041 cgaaaacacc gaggagacat ccgatgaacc ggtcgcgcag ggtgtttgac gacgcatgaa
     2101 cggacccgac gacaaggagc tgaaaaaggg cgggatgatg cgccaggcgg aggccgggct
     2161 cttctcggtc cgcctccatg tggtgggggg ccgcctcgcc acgccgcagc tccgggcgat
     2221 ccaggaagcg gcggaccggt tcgggcgggg ggagatccac ctgaccagcc gtcagggagt
     2281 ggagattccc cacataccgc agggtgccct cgctgcgctg aaggagttcc tcgcaccctc
     2341 cggtgtcggg gagggcgtcg ccggccccac ggtccgcacg gtaacggcct gccaggggtg
     2401 ccgcgtctgc ccgagagggg tgatcgattc cccggcgctg gccgaggcgg tcgacaggga
     2461 gctctacgga aagccggtgc cgcacaagtt caaggtcggc atctccgggt gcgtcaacaa
     2521 ttgcatgaag gccgaggaga acgacgttgg gatcaaggga tggatcgagc cgcgctggga
     2581 agtgccggcc tgcaccttct gcggaatctg ccagaccgtc tgcccgacga aggtgatctc
     2641 gatgtcgggg gacggccacg cgttgatcgt cgactcgacg ctgtgcatcg ggtgcggcga
     2701 ctgcatcacc tcctgcccca ccgggagcat gcgggagcag atccgcgggt accggatctt
     2761 cgccggcggg aagttcggca ggagaccctc cctcgggaag aggatcctcg gggtcttgcg
     2821 gaggaaggag gatgcgatga aggcgattcc ggccgtcctc gacttcttcc gcgagcatgg
     2881 aaagccgagg gagcggttcg gggataccct ccagaggacc gggcttccgg cgctggtgac
     2941 attcgtgaac gaacggacgg ggctttcatg aaacgaagac gggggacctc ttgaaaaaga
     3001 accgcagcgt cgaaacgatc gccgcgcagg cgggggtggg ggcggacaag gcgttcggcg
     3061 cggtcagcgt cccgatctat ctgtccgcga tctaccggta cgagcgcttc gggaagaatc
     3121 ggggattcga ctactcgcgg ggagagaacc cgacgcgcaa ggccgtggag aaggctctgg
     3181 cggatctcga aggaggatcc cgcgccgtcg ccttttcctc ggggatggcg gcgatctccg
     3241 ccctgatgac cctcttccgg acgggagacc acatcctctg ttcggacgat ctctacggcg
     3301 ggacgtaccg cctgttcgag aagcttttgc gcccgtacgg tctctccttc gactacgtgg
     3361 acatggggaa tccggccgcc gtgaggaaaa agatccgcgg gaagacgaag gcgctgttca
     3421 tcgagacgcc gacgaacccc ctgatgaaga tcgccgacct gaaaggggcc gcgcggatcg
     3481 gccgggaaaa aggggtcctg accgtcgttg acaacacgtt catgtccccc ctgctccagc
     3541 ggccgcttgg cctggggatc gacgtggtcg tccacagcgc caccaagttc ctgggagggc
     3601 acaacgacct gatcggcgga gcggtcgtca c
//
LOCUS       DASVJL010000352         6717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1466481, whole genome shotgun sequence.
ACCESSION   DASVJL010000352 DASVJL010000000
VERSION     DASVJL010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466481"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="VF357_07405"
     CDS             complement(<1..1493)
                     /locus_tag="VF357_07405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7524012.1"
                     /translation="MDPEKEILQIADTALIRSVAKRLMESSGVEEGLAGILPLLIRFL
                     RSDFGAFLVRRESGWIVVGRGADEAFYRTLPRTARLVARATPIVLSAITGLLSKRRES
                     REGSPDSVVLPVHGKCGRMGYGVFRTGEGAVPTHGTLHLLDLVGAQVGAFCENAFRCE
                     RAENDKGLLFRANEELECLLRFNESLHAIGDADSMFKWLSREIERFAPVLGIEMVSLI
                     GRSVLRIGCAPGTKVPAGEAGEESCVVARKCSETLASRYRIDAPADGFKIKRFECLAA
                     SDSYRPATARRRVETLLLHAGKVVGFLAVEMPATSEDSPFIDRLIASVAGQIGLFLHN
                     NADREKIRSLASRDALTGLYNFRSFQEIFDREFERFLRHGRNLSLMMIDLDNFKRIND
                     TFGHQAGDHALCEVARILLANLRKTDYAFRYGGDEFVVLMAETDAERAAVFAQRIRAS
                     VKREVRELPHCEFSFSTSIGIADCSNLKSGDRGELLRLADEALYLAK"
     gene            complement(1651..2913)
                     /locus_tag="VF357_07410"
     CDS             complement(1651..2913)
                     /locus_tag="VF357_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEX7524013.1"
                     /translation="MLTSLYTAISGMDATGTQLSVIGDNIANMNTVGFKKSTVAFGDV
                     LSQSIANVSGSGQIGRGVFVQEISPLMTQGAFETTENALDLAIDGDGFFAVSDNGARS
                     YTRAGNFSVNRDGFIVTPDNLRLQGYLADGAGKISGTTGDLQLTATQNPARMTGAAKV
                     AVNLDATAPVQAAAFTLDGDGNGTPNDPINYNSSTTVSMYDSQGGVHQVTLYYAKTAD
                     NTWDVHDVYEDPANPGQLLEASGSPQTLTFDTSGALTTVSAGSPTFDFGGGVTGSQGI
                     TFDHTGTMQYASSFAVLKLDQDGYSSGALKNLIISDAGVMTGNFTNGQTRLIGQVAMA
                     RFIAPTELVKLGRNLYGESYASGQPIVSAPGTSGLGRIASNTLELSNVDLAVEFVKMI
                     SAQRGFQANSRIITTTDELMQEVVNLKR"
     gene            complement(2939..3370)
                     /locus_tag="VF357_07415"
     CDS             complement(2939..3370)
                     /locus_tag="VF357_07415"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEX7524014.1"
                     /translation="MDVTALNNGTAAATSATNAASQNTVTKDEFLKLFVTQLKNQDPL
                     NPMDSTGFTTQLAQFSSLEQLTNINGGITSLLASQNSLQNASAANLIGKQVGFQVKDA
                     NGKAGTMQHGTVTGVAFDAGKTSFVVDGATKIALGDIQEIQ"
     gene            complement(3384..3962)
                     /locus_tag="VF357_07420"
     CDS             complement(3384..3962)
                     /locus_tag="VF357_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX7524015.1"
                     /translation="MCFSNPDPAPEGTRGAEGARRKKDDTSARIEGQSEPATTPPADG
                     KLGVAEKLAPPRPVGPASDPGIVRFEEKAFVITRKSDTSVEVTLAPPGVGKLEIEVVL
                     EKGVVNARILAADSAGREAIARSLPQIVEALARDGMNVGGFTVSLKERRDRTGGEPGH
                     GALRDPDSRLLSAVTQAASASAASAGLVDIFV"
     gene            complement(4859..5248)
                     /locus_tag="VF357_07425"
     CDS             complement(4859..5248)
                     /locus_tag="VF357_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524016.1"
                     /translation="MPVQPKKAPDLAERERALKAEEERLLVLRKEVDMKVATYEKLLG
                     QMEEKQKRKQEEKDAKVDQLVKLFEGMPPEGAAARLAALDDGMAAAILGKMKSRKASA
                     ALSVMDPKRVAVIALRIVGRVKNFPSE"
     gene            complement(5365..5805)
                     /gene="fliJ"
                     /locus_tag="VF357_07430"
     CDS             complement(5365..5805)
                     /gene="fliJ"
                     /locus_tag="VF357_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEX7524017.1"
                     /translation="MNRLRTIGKVLQLKENREEEIGCEVRALRNAVGADQTRLGILEN
                     TYRDTLEIFNRKQREEGLAPHEMGIYYSYLFHLNKAMEAKKAEIAQTLSELDVRQGEL
                     VAAHMETRVVESLKDRRSREYEKEDARRERKEMDFISSTRRVGQ"
     gene            complement(5780..>6717)
                     /locus_tag="VF357_07435"
     CDS             complement(5780..>6717)
                     /locus_tag="VF357_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEX7524018.1"
                     /translation="RITHPIDLGIRSLNGLLTCGKGQRIGIMAGPGVGKSVLLGMIAK
                     YTDASVNVIALTGERGREVREFIDRNLGEEGMRKSVVVVSTSEQPALMKLRAAFTATA
                     IAEYFRDCGHDVLLFMDSLTRVAMAQREIGLALGEPPTTKGYTPSLSTILSKLLERAG
                     TTEGKGSITGMYTVLVEGDDLSDPVADASMAILDGHIVLSRELAGENQYPAVDILRSI
                     SRVMPDIVEPAHKEAASRFRELLATYRKHEDMISIGAYKEGSNPKVDRAIRMIDRLKG
                     YLRQGMNEPQNYRESVAQLYRLFEEASDEPAPNNR"
BASE COUNT         1074 a         2118 c         2180 g         1345 t
ORIGIN      
        1 ttcttcgcca agtagagagc ctcgtcggcc aggcggagga gttccccgcg atcccccgac
       61 ttaaggttgc tgcagtcggc gattccgatg ctcgtcgaga aagaaaattc gcagtggggg
      121 agttcgcgga cctcccgttt caccgacgcg cggatccgct gggcgaaaac ggcggccctc
      181 tccgcgtccg tctcggccat cagcacgacg aactcgtcgc cgccgtaccg gaaggcgtag
      241 tcggtcttcc gcagattcgc caggaggatt ctcgcgacct cgcacagggc gtggtcgccg
      301 gcctggtggc cgaaggtgtc gttgatccgc ttgaagttgt cgagatcgat catcatgaga
      361 gacaagttcc ggccgtggcg gaggaaccgc tcgaactccc ggtcgaaaat ttcctggaac
      421 gaccgaaagt tgtacaaccc ggtgagagcg tcgcgactgg caagggaccg gatcttctcg
      481 cggtcggcgt tattatgaag gaacagcccg atctggccgg ccaccgaagc gatcaacctg
      541 tcgatgaacg ggctgtcctc cgaggtggcc ggcatctcga cggcgaggaa ccccacgacc
      601 ttgcctgcgt ggagaagcag tgtctcgacc ctccgtcgag ccgtcgcggg ccgataggaa
      661 tccgacgcgg cgagacactc gaaccgcttt atcttgaacc cgtccgccgg ggcgtcgatc
      721 cggtagcggg atgcgagggt ttccgaacat ttccgcgcga cgacacaact ttcctcccct
      781 gcctcccctg ccggaacctt cgttccaggg gcgcagccga ttcgcaggac ggacctcccg
      841 atcagggaga ccatctcgat gccgaggacg ggggcgaacc gttcgatctc ccggctcagc
      901 cacttgaaca tcgagtccgc gtccccgatc gcatgaagcg actcgttgaa gcggaggaga
      961 cactcgagtt cctcgttcgc gcggaaaagg aggcccttgt cgttttccgc ccgctcgcaa
     1021 cggaaggcgt tctcgcagaa cgcccccacc tgggcgccga cgaggtcgag caggtggagg
     1081 gtcccgtgcg tgggaacggc tccttccccc gtccggaaga ccccataccc cattcggccg
     1141 cacttgccgt gcacgggcag gacaacggag tcaggagatc cctcccggct ttcccgacgt
     1201 ttggaaagga ggccggtgat ggcggacagg acgatgggcg tcgcgcgggc cacgaggcgg
     1261 gccgtgcgcg ggagggtccg gtaaaatgcc tcgtcggccc ctcggccgac cacgatccag
     1321 ccgctttccc gccggacgag gaatgccccg aagtcggacc tgagaaatcg tatcagcaga
     1381 ggaaggatgc cggcgagtcc ttcctcgacc ccactcgact ccataagacg tttcgcaacg
     1441 gatcgtatca gggcagtgtc ggcgatctgc aggatttctt tttccggatc catgattcct
     1501 ccctatctca cgaggtccga aatcgttagc cgaattcaag catttaccgt gccatttaac
     1561 gtactggctc gttatcgttt acaaatctcg tgttcctatc ggttggttcc cctgagcgct
     1621 caggggaatg tcaacggttt ggctctatca ttacctcttg aggttcacga cttcctgcat
     1681 cagctcgtcc gtggtcgtga tgatccggga attcgcctgg aacccccttt gggccgagat
     1741 catcttgacg aattccacgg ccaggtcgac gttgctgagt tcgagcgtgt tcgaggcgat
     1801 gcggccgagc ccggaggtcc ccggggcgct cacgatcggc tgcccggagg cgtacgattc
     1861 cccgtagaga tttcgcccga gtttcaccaa ctccgtcggg gcgatgaacc tcgccatggc
     1921 gacctggccg ataagccgcg tctgcccgtt cgtgaagttg cccgtcatca cgccggcatc
     1981 ggagatgatc aggttcttga gggccccgga ggagtagccg tcctggtcga gcttcagaac
     2041 cgcgaagctc gaggcatatt gcatcgttcc cgtgtggtcg aaggttatgc cctggctacc
     2101 cgtcacgccg ccgccgaagt cgaaggtggg agagccggcg gatacggtcg tcagagcccc
     2161 gctcgtgtcg aacgtcagtg tctgcgggct ccccgaggcc tcgaggagct ggccgggatt
     2221 ggccggatcc tcgtagacat cgtggacgtc ccaggtgttg tccgcggtct ttgcgtagta
     2281 gagggtcacc tggtggacgc cgccctgcga gtcgtacatc gagaccgtcg tggaggagtt
     2341 gtagttgata ggatcgttcg gcgttccgtt gccgtcgccg tcgagggtga acgcggcggc
     2401 ttggacgggg gcggtggcgt cgaggttcac ggcgaccttc gccgcgccgg tcatccgtgc
     2461 ggggttctgc gtggccgtga gctgaaggtc gccggtcgtt ccggagattt ttcctgcgcc
     2521 gtcggcgaga tacccctgga ggcggaggtt gtcgggggtc acgatgaagc cgtcgcggtt
     2581 gacggagaag ttcccggccc gcgtatagga cctcgcaccg ttgtcgctga cggcgaagaa
     2641 cccgtcgccg tcgatcgcga ggtcgagagc gttctccgtg gtctcgaacg ccccctgcgt
     2701 catcaggggg gagatctcct gcacgaagac gccgcggccg atctggcccg aacccgagac
     2761 gttcgcgatc gactggctaa ggacgtcgcc gaaggcgacg gtgctcttct tgaatccgac
     2821 cgtgttcatg ttcgcaatgt tgtccccgat gacggagagc tgggtgccgg tggcgtccat
     2881 cccgctgatg gcggtgtaaa gtgacgtaag cattttcttc ctcctcctga ttcctggttt
     2941 attggatttc ctggatatcg cccaatgcaa tctttgtggc tccgtcgacg acgaacgagg
     3001 tcttgccggc atcgaaggcg acgcccgtca cggtgccgtg ctgcatcgtc ccggcttttc
     3061 cgttcgcgtc cttcacctgg aaaccgacct gcttcccgat gaggttcgcg gcggaagcgt
     3121 tctggaggga attctgggag gcgagcaggg acgtgatgcc cccgttgatg ttggtgagtt
     3181 gctccagcga gctgaactgg gcgagctggg tggtaaaccc cgtgctgtcc atcgggttca
     3241 acggatcctg gttcttcaat tgcgtcacga acagcttcag gaactcgtcc ttggtgaccg
     3301 tgttctggga agcggcgttc gtggccgacg ttgcggcggc cgtgccattg ttcaaagcgg
     3361 ttacgtccat gggttcccct ttctcagacg aagatgtcga caagccccgc cgatgcggcg
     3421 gaggccgatg ctgcctgcgt tacggcggac aggagtcggg agtccggatc gcgcaacgcg
     3481 ccgtgccccg gctcgccccc cgtccggtcc cgtctctctt tcagcgaaac cgtgaaacca
     3541 ccgacgttca tcccgtcccg ggcgagcgcc tccacgatct gaggaaggct tcgcgcgatc
     3601 gcctcccggc cggcggaatc tgcggcgagg atccgtgcgt tgacgacccc cttctcgagg
     3661 acgacctcga tctcgagctt tccgacgccc ggcggtgcaa gggtcacctc gaccgaggtg
     3721 tcgctcttcc gggtgatgac gaaggccttc tcctcgaacc ggacgatgcc cgggtccgac
     3781 gcggggccga cgggccgagg cggggccagc ttctctgcaa ctccgagttt tccgtcggcg
     3841 gggggcgtgg tggccggttc ggactgtccc tcgattcgag cggaggtgtc gtctttcttt
     3901 ctccgggcgc cctcggcgcc ccgcgtcccc tccggggcgg ggtcggggtt gctgaaacac
     3961 acgggggtcc catcgactct cacggagccg gcatttttcg gttcggacgc cgcggcggtg
     4021 gggggagcgg aaaccgttcc ggggccaccg tccttggttg cttgcgccgg tgctgcgttc
     4081 tccccaccgt ccgccgcagc ggggtgaatt ccggaagcga gctgtttcag gagcggatcc
     4141 ccctccgcgg acaacgtagc cgcgcccgcg aggacaggga ccggatcggc tttcgcagag
     4201 gcggcacctt tcggttcgga caccgcggcg ggtaggggcg cgggaacggc tccggggccg
     4261 atggccctgg gcgtttgcgc ctgcgcggcc ttctcctcac cgttcgccgc agcggggtga
     4321 attccggaag tgagctgtgt caggagcgga tccccgtccg ggggcaaggt tcctgcgcct
     4381 tcgaggacag cggccgggcc ggttttcgca gagtcggcat ctttcggttc ggatgccgcg
     4441 ccctcggcgg ccggcaggga atcgtccggt gcggggggag cgaccgaacc ccccacggca
     4501 gcggtaacgg aaggaggtgt ggaggcgtcc gccttggctt ccttcccggc gatcgccgcg
     4561 cggcgaccgc cctgggcgga gggccggcct ttcgcagggc gatcctgtcc ggcggcggtg
     4621 ctcttcagga tgctctcgaa ccggtgtcca ccgttgtgcc ccgtgtccag ggaacccggc
     4681 ctttcccccg gttcagagaa gcctcgtgcc gccgaagcag cggatgggat gaaagatggc
     4741 aggatcggtt ccatgacctt caacgttaca actaccgtgc cacttcgtat aactcgttaa
     4801 ttatcaaaga atttcaagaa aaaatggggt tggcgaggag aggtgggaag aaaaaatatc
     4861 actccgaggg aaaatttttc actctgccga cgattctcag tgcgatgacc gccacccgct
     4921 tcgggtccat gaccgacaga gccgcgctgg ccttccggct cttcatcttg ccaaggatcg
     4981 cggcggccat cccgtcgtcg agagcggcaa ggcgcgcggc cgctccttcc gggggcatcc
     5041 cctcgaaaag cttcacgagc tggtcgactt tggcatcctt ttcttcctgc ttccgcttct
     5101 gtttctcctc catctggccg agaagctttt cgtatgtcgc gaccttcatg tcgacttcct
     5161 tgcggagaac gaggagccgt tcctcctccg ccttcaaagc cctctcccgc tcggcgaggt
     5221 cgggggcctt cttcggctgg accggcatcg aagtctcctt gggtgccgcg gaggggggcg
     5281 ctgcggaggg gggcgccgcg gctgacgacg ggacggggca caacaaggca gcaaggagaa
     5341 tcgccgcagc ggcgcgatgc gccgtcactg cccgaccctc ctcgtggacg agataaaatc
     5401 catctccttg cgctcccgcc gcgcgtcttc tttttcgtat tcccgggatc gccggtcttt
     5461 caacgactcc acgacccgtg tctccatatg cgccgcgacg agttcaccct gcctcacgtc
     5521 gagctccgac agggtctggg cgatctccgc cttcttcgcc tccatcgcct tgttgaggtg
     5581 gaagaggtag ctgtagtaga tccccatctc gtggggcgcc agcccctctt cgcgctgctt
     5641 ccggttgaat atctcgaggg tatccctgta ggtgttttcg aggatgccga gtcgtgtctg
     5701 gtccgcgccg accgcgttgc gaagcgcacg gacctcgcac ccgatctcct cctccctgtt
     5761 ttccttgagc tgtagaacct taccgattgt tcggagccgg ttcatcgctg gcctcctcga
     5821 agagtcggta gagttgcgca acgctttcac ggtaattctg gggttcgttc atcccctgcc
     5881 ggaggtaccc tttcagccgg tcgatcatcc ggatggctcg gtcgaccttg ggattggacc
     5941 cctccttgta ggcgccgatg ctgatcatgt cctcgtgctt gcggtacgtg gcaaggagct
     6001 ccctgaagcg gctcgccgcc tccttgtggg ccggctcgac gatatccggc atcacccggc
     6061 tgatggaacg gaggatgtcg acggcggggt actggttctc gccggcgagt tcccgcgaca
     6121 ggacgatgtg gccgtcgagg atggccatcg aggcatccgc gacgggatcc gagagatcgt
     6181 ccccctcgac gagaaccgtg tacatcccgg tgatgctccc cttcccttcc gtcgtgcccg
     6241 cccgctcgag gagcttggag aggatggtgg aaagggaggg cgtgtaccct ttggtcgtcg
     6301 gcggctcgcc cagggcgagg ccgatctccc gctgcgccat cgccacgcgc gtgagcgagt
     6361 ccatgaagag gaggacgtcg tgaccgcaat cacgaaagta ttccgcgatg gccgtggccg
     6421 tgaacgcggc gcggagcttc atcaaggccg gctgctcgga cgtcgacacg acgacgacgg
     6481 acttgcgcat cccttcctca ccgaggttcc ggtcgatgaa ctcgcggact tccctccccc
     6541 gctcgccggt cagcgcgatg acgttgacgg aggcgtcggt gtacttcgcg atcatcccca
     6601 ggaggacgct cttgccgacg cccgggcccg ccatgatccc gatgcgttgc cccttcccgc
     6661 aggtaagaag accgttgaga gatcggatgc cgaggtcgat cgggtgcgtg atcctgc
//
LOCUS       DASVJL010000353         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_339554, whole genome shotgun sequence.
ACCESSION   DASVJL010000353 DASVJL010000000
VERSION     DASVJL010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339554"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VF357_07440"
     CDS             complement(<1..1271)
                     /locus_tag="VF357_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7524019.1"
                     /translation="MDKVRVRDLAKELGMETSKEVLAFLERIGVKGKSASSNLEGEMI
                     DRVRAHFRKPAPPPPPPRTTTLTRSDGVLERRSKNVVLRRGTPTPPPVPELPPEEPPA
                     PPVVVEETAPPVVEPVPVAQVPAGAPPEKVAEPMIEPPIPEPTVRVIEKPAAPPKPED
                     VRKEKEKKWKKARPHEKKVQKAVLKQTIIQEVLAEPEVDAKKAEGVTPAPEVVVRQFQ
                     PGRAAKRRGGRPVREKKAPSTVPPKASKRLLKIEEVVTVGDLAHRMGVKAADVIKKLI
                     EMGMPTTVNQLLDADTAALLAQEYGFVVENVAPEVERLIDQEEDRAEDLQPRPPVVTI
                     MGHVDHGKTTLLDAIRESNVTGSEAGGITQHIGAYEVEHNGRRVVFLDTPGHEAFTSM
                     RARGASVTDIVILVVGADDGVMPQTVESIDHA"
     gene            complement(1283..1537)
                     /locus_tag="VF357_07445"
     CDS             complement(1283..1537)
                     /locus_tag="VF357_07445"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX7524020.1"
                     /translation="MQCGAVRDKDGLLRIAGKPGMGWTPDPGGNLPGRGIYLCPVGEC
                     VDRFAARIRTPKGGARWKMGVEGKELADRLAASRRGGQEK"
     gene            complement(1563..>2631)
                     /gene="nusA"
                     /locus_tag="VF357_07450"
     CDS             complement(1563..>2631)
                     /gene="nusA"
                     /locus_tag="VF357_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX7524021.1"
                     /translation="FEILSFRTATETVVDPDTQMTLEEAQVLDPEAQVGDSLGERLST
                     KDLGRIAAQTARQIIMQKVKDAEREVIFNEFIGRKGEILNGIVQRYERDCLVIDLGKT
                     EAIMPPSEQIPRERYRQGDRIRAYIKDVTKTSRGPEILLSRADPRVILKLFEQEVPEV
                     YEGVVSILSVAREPGFRTKIAVRSRDRDVDPVGACVGMKGSRVQSVVQELRGERIDII
                     AWDEDPARFVCNALQPAEIIRVLVNESGKSMEVVVPEEQLLLAIGKRGQNVKLASKLV
                     GWHIEVRAEGQVEDALKRAEGLFTKKPEGMAAEVPPPEGEPSVAPAVESDAGAKEGET
                     AVSPPAEGMDVDSKAGEPTKE"
BASE COUNT          347 a          960 c          825 g          499 t
ORIGIN      
        1 gcgtggtcga tcgattccac cgtctgcggc atcacgccgt cgtccgcccc gaccacgagg
       61 atgacgatgt cggtcacgga ggcgccccgg gcgcgcatcg aggtgaacgc ctcgtggccc
      121 ggcgtgtcga ggaagaccac ccgccggccg ttatgctcca cttcataggc cccgatgtgc
      181 tgggtgatcc cgcccgcctc cgacccggtg acgttgctct cccggatggc gtcgagcagc
      241 gtggtcttgc cgtgatcgac gtgccccatg atggtgacca ccggcggccg cggctgaagg
      301 tcttccgcgc ggtcctcctc ctggtcgatc aatctctcca cctcgggggc gacgttttcc
      361 acgacgaaac cgtactcctg cgcgaggagc gcggcggtgt cggcgtcgag gagctgattg
      421 acggtagtgg gcatccccat ctcgatcagc ttcttgatga cgtcggcggc cttgaccccc
      481 atgcgatggg ccaggtcccc gacggtcacg acctcctcga tcttcagcag ccgcttgctc
      541 gccttgggcg ggaccgtcga gggggccttc ttctcgcgta cgggccgtcc ccccctcctc
      601 ttcgcggcgc ggccgggctg gaactgccga accacgacct cgggcgcggg cgtgacgcct
      661 tccgccttct tcgcgtcgac ctccggctcg gcgaggactt cctggatgat cgtctgcttg
      721 aggaccgcct tttgcacctt cttctcgtgc ggcctcgcct tcttccactt cttttccttc
      781 tctttccgca catcctccgg cttggggggc gcggcgggct tttcgatcac gcgaaccgtc
      841 ggctccggga tcggcggctc gatcatcggc tcggcgacct tttccggcgg cgcccccgca
      901 ggaacttggg caacgggaac gggctcgacg acgggcggcg ctgtttcctc gacgaccacg
      961 gggggcgcgg gaggctcttc ggggggcagc tcggggacgg ggggcggagt cggagttccg
     1021 cggcgcagga ccacgttctt ggagcggcgt tccagcaccc cgtcggagcg ggtcaacgtc
     1081 gtggtgcggg gcggcggcgg cggcggagcg ggtttccgga aatgggccct gacccggtcg
     1141 atcatctccc cctcgagatt gctggacgca gactttccct tgacgccgat cctctccagg
     1201 aaggcaagga cctccttgct ggtttccatc ccgagttcct tggccagatc acgcacgcga
     1261 accttgtcca tccactcccc ctctactttt cctgcccgcc tcgccgcgat gcggccagac
     1321 ggtccgccag ttccttgcct tcgacaccca tcttccagcg ggccccgccc ttcggcgttc
     1381 gtatcctcgc cgcgaaccga tcgacgcact ccccgacggg acagagatag atcccccggc
     1441 ccggcagatt ccccccaggg tcgggcgtcc agcccatccc cggtttcccc gcgatccgga
     1501 gcagcccgtc cttatcccgg acggcgccgc actgcacaca ggtgcgctgc ggccccgaag
     1561 cgctactcct tggtgggctc ccccgccttc gaatcaacgt ccatgccctc cgctgggggg
     1621 gatacggcgg tctccccctc cttcgcgccc gcatcactct ccacggcagg agctacggaa
     1681 ggctcgcctt cgggcggcgg gacctccgcg gccatgccct ccggcttctt cgtgaacagc
     1741 ccctccgccc gcttcagggc gtcctcgacc tgcccctcgg cccgaacctc gatgtgccag
     1801 ccgaccagct tggaggcgag cttcacgttc tgcccccgct tcccgatggc gagaagcagt
     1861 tgctcctcgg gaacgaccac ttccatgctc ttcccggact cgttcacgag cacccggatg
     1921 atctcggccg gctggagggc gttgcagacg aaccgggcgg ggtcctcgtc ccaggcgatg
     1981 atgtcgatcc gctcgccgcg aagctcctgt acgacgctct gcacgcggga ccctttcatg
     2041 ccgacgcacg ccccgacggg gtcgacgtcc cggtctctgg agcgcacggc gatcttggtg
     2101 cggaagccgg gctccctcgc cacgctgagg atggagacca ccccctcgta gacttcggga
     2161 acctcctgtt cgaagagctt gaggatcacc ctcgggtcgg cccgcgagag aaggatctcc
     2221 ggaccccggg acgtcttggt gacgtccttg atgtaggccc ggatccggtc gccctggcgg
     2281 tatcgctcgc ggggaatctg ttccgacggg ggcatgatcg cctcggtctt cccgagatcg
     2341 atgacgaggc agtcgcgctc gtaccgctgc acgatgccgt tcaggatctc ccccttccgg
     2401 ccgatgaact cgttgaagat aacctcgcgc tccgcatcct tcaccttctg catgatgatc
     2461 tggcgcgccg tctgggcggc gatccgccca aggtccttgg tgctcagccg ctcgccgagg
     2521 ctgtcgccca cctgcgcctc ggggtcgagg acctgcgcct cctcgagggt catctgcgtg
     2581 tcggggtcga cgaccgtctc cgtcgccgtg cggaacgaca ggatctcgaa c
//
LOCUS       DASVJL010000354         9598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_527794, whole genome shotgun sequence.
ACCESSION   DASVJL010000354 DASVJL010000000
VERSION     DASVJL010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527794"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VF357_07455"
     CDS             <1..1074
                     /locus_tag="VF357_07455"
                     /inference="COORDINATES: protein motif:HMM:NF044849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524022.1"
                     /translation="LVDHVVSTFAEFAGIPGLVKDAKPHVGTDRLRHFCRRMRAELAS
                     LGAVVRFGARVEELSVSEGEVRGVLLAGGGEVRSPVVLLATGHSARDTVRRLFAQGVG
                     MSGKAVAVGFRVEHPQAMIDRIQYGPMAGKGLPPAEYRLAARAPSGRGVYSFCMCPGG
                     EVMNAASGEGQSPVNGMSVRARNSGFGNSGIVASIASAEFGEAGPREGGPLAGIGFQE
                     EIEHRAFDRGGGGYGVPAANLLAFVRGKELPPSPGRLLAPRVVPDDLRGILPGAVEED
                     IRFGLLRFGGAMRGFLTREATLYAVESRTSSPVRIERVNYESVTHKGLYPVGEGAGHA
                     GGIVSSAVDGIRAALRILETYSS"
     gene            1090..2067
                     /locus_tag="VF357_07460"
     CDS             1090..2067
                     /locus_tag="VF357_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7524023.1"
                     /translation="MQKTRFVKDLKEGEPVRDLFLVANKALLTSNAGKPYLTLQLRDR
                     TGQIEARVWDRAEEIGKRFDRDDVVEVGGTAIAYQGRVQLKVHDVRREEGGTKDLSEY
                     LPVTKKGIEPLWKTLQEYIGGVKDKDLARLLAAVFPDPPETDVARRFRQAPGGKTMHH
                     DYIGGLLEHTVSVAGICRMLSAHYEGVDADLLLAGALLHDVGKVHELSYEGAFDYTDE
                     GRLLGHLYMGTEYVSRVCADLPGFPPEKTMLVKHIILSHHGELEYGSPKRPKTLEAIL
                     LHHVENMDAKATAFGDAIAELREGVRWTDYQRMFERYLFSGKFPQGGSS"
     gene            2117..3262
                     /gene="bioF"
                     /locus_tag="VF357_07465"
     CDS             2117..3262
                     /gene="bioF"
                     /locus_tag="VF357_07465"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX7524024.1"
                     /translation="MEAWKAFLEERERRQLLRRLVPSSGRAPALAVRDGREYVDFSSN
                     DYLGLSSHPALVAAAREALDRYGVGSGASRLMSGDLEIHHELEDSVAVFKGSEAALVF
                     NSGYQANTGIIPAMFGRRDAVFADQFCHASQLDGALLSRARLLRFRHNDPDHLDRMLG
                     KHRGAFERALVTTESVFSMDGDIAPLSALLAVCRRHRCLLMVDEAHATGVFGPQGRGC
                     VEAEGLAGQVDLVMGTFSKALGGFGAYLAASRTVIDYLVNTARSFIYSTALPPPVIAA
                     DLAALRLCLSGETRGEELLRRAGAFRGALRGKGWTVGGESQIVPVVVGESALAVSLSI
                     TLAGQGFLALPVRPPTVPEGSARLRFSLTTAHTDRQIAAVVEALGAA"
     gene            3252..3941
                     /locus_tag="VF357_07470"
     CDS             3252..3941
                     /locus_tag="VF357_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7524025.1"
                     /translation="MPREEFTVAGEGDPSMVLLPGWATDGRIFEGVLPGVAAVTTGPL
                     RPGGFSRRLAVFLDRMACGPVTVVGWSLGGFLAAEFAREYPDRVRRVVLVGIRRKYPE
                     GDVEAVLRSLSADPGNCLSGFYAQCFYPSQMSAYRRFRGGLQAAYLREMDGGALREGL
                     SYLEGARLSGATLPACPVVIVHGEKDVVAPIAEAEGVARESGNATFHRLPGAAHAAFL
                     ADGFRAVAAGG"
     gene            3934..4836
                     /locus_tag="VF357_07475"
     CDS             3934..4836
                     /locus_tag="VF357_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7524026.1"
                     /translation="MVDPDVLRRFSAGAARYDAHAHVQRLSAVDLLAYTEASLGPSPN
                     GGLARTMNILEPGCGTGLYTRMLLDAFPAASIFGIDVSEAMIRVARKRIDDQGWSSAA
                     TGRDAPGLVVAAGDPRARFAVADAEEIDAGSYDLVTSNAVFQWFLSLPRTLGRMASLL
                     RGGGLLTFSFFGPETYAELADALRAAAGGNGRPGAAAGRDAPGFVAAAAFHSREEISG
                     ALSAAFPRWEVVERRYHQEFPTLAALLRSIRYTGTRGGGAREAWSPGKLARVEEAYRG
                     RDGGIRATYQVFLCRGVIPGGETG"
     gene            4833..5540
                     /gene="bioD"
                     /locus_tag="VF357_07480"
     CDS             4833..5540
                     /gene="bioD"
                     /locus_tag="VF357_07480"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX7524027.1"
                     /translation="MKGVFITGTGTGVGKTVVCGLLAGFLRARGMRVTTQKWVETGVT
                     DGPSDIDVHRRLMGGPGPAPEPPPADRCPYRFSLPASPHLAAAREGRRVAPVVIEAAY
                     RRLAESHDAVLVEGAGGFLVPLSEELLTGDLVARIGLPVLVVAGNRLGCVNDALLTVE
                     AVRRRGISLQGIVFNRLPGEGDGTRAEVLADNPRIVAEITRAPVLGEVPLLPDPARGA
                     EAFAPVGRAFLERWRST"
     gene            5540..6886
                     /gene="bioA"
                     /locus_tag="VF357_07485"
     CDS             5540..6886
                     /gene="bioA"
                     /locus_tag="VF357_07485"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF004624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX7524028.1"
                     /translation="MDRKSWLRKDLRHIWHPYTQMSTLATEPPMLIDRAEGLFLFDAD
                     GNRYYDAISSWWCIVHGHGHPKIREAVTRQMERLDHVLFAGVTHEPAVRLASRLAAIA
                     PEGLSRVFFSDNGSTAVEVALKMSLQCWSNLGREERTGFVCLDHGYHGDTTGCMSVSG
                     VEAFLRAFRPILFPARRVPAPYCYRCPVGKTYPECGVSCVDVLGDALREGGETIAAVI
                     LEPLLLGAGGMIVYPPEYLSRAAALARKHGAHFILDEVATGFGRTGTMFACEQAGVSP
                     DILCLSKGLTGGTMPLAATLTTAEVYNSFLGGPDSGKTFYHGHTYTANPVGCAAALAS
                     LDLFEEEELVDRVARLAPRLAEGVRELAGLPLVGDVRGIGMVAALELVRDKGTKEPLP
                     GTAPLFRDIRREGLDRGLFLRPLGNVVYLFLPQAVTREALDDILDRFGETLRKVLS"
     gene            6883..7620
                     /locus_tag="VF357_07490"
     CDS             6883..7620
                     /locus_tag="VF357_07490"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX7524029.1"
                     /translation="MTREMGAARGWVRDCRSLCVLTGAGVSAESGVPTFRGPEGLWKM
                     RDPTTLATPEAFAADPGEVWEWYQWRRQRIRGCAPNPGHTALSAVEAAIPDFLLVTQN
                     VDGLHRAAGSRRVAEIHGSIWVVRCTGCGAEREERDDFAELPPRCAACGGMLRPGVVW
                     FGETLPTGLSDAVMEMLSRCEVLIVAGTSAVVSPASGYAAVAKRRGARVIEVNPDETP
                     VSALCDATFRGKSGEVLPALFGHPKSS"
     gene            complement(7602..8972)
                     /locus_tag="VF357_07495"
     CDS             complement(7602..8972)
                     /locus_tag="VF357_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7524030.1"
                     /translation="MFFDTHAHLDLSPLYDAEEEVVRRARDAGVTRIATVGIDPESGE
                     RAVSIAHRHTGVYAVVGLHPHDAGRLSDKLLARLEALSRCDKVVAIGETGLDFHRDRA
                     PRDSQRAAFREQIRLARRRGLPVVIHDRDAHDEVLSILSEENAAEVGGIIHCFSGDLA
                     MARRALGMNFLVSIPGAITYKGSEKQVEAVRELPLDRLLIETDCPFLAPIPHRGKPNE
                     PSYVPLVAAKIAAIKGVTVEDVARTTTINALRLFRIPVEEEVRITYKIRDSLYLNITN
                     RCTNACTFCPKRHDYHVKGHLLKLPGEPTAAEALAEIGDPTKFDEVVFCGFGEPLLRL
                     DVMKEIAAELKRRGARVRVNTDGLGNLVHGRNILPEIAGLVDALSVSLNAPDAQTYAR
                     ICPNRYGAASFAALLDFLREAPKHVPKVSATAVALPGLDPEAVRRLAESIPGVSFRLR
                     PYDDLG"
     gene            complement(9001..>9598)
                     /locus_tag="VF357_07500"
     CDS             complement(9001..>9598)
                     /locus_tag="VF357_07500"
                     /inference="COORDINATES: protein motif:HMM:NF039287.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7524031.1"
                     /translation="GADAFRYFLLREVSFGLDGDFSEKELIKRANSELADKLGNLLNR
                     SLGMLGKYFAGVVPEPGPAEPEDAVLFACGRKSLPAVAAAMDEVAFHKALGAIIELVT
                     KGNEYVQSAQPWALAKDPGKRARLGTVLYNALEASRVAALMMAPFTPTAAQKLWEALV
                     PEGGAVENVRIDRDGAWGGLAAGATLPNACIVFPKIEV"
BASE COUNT         1314 a         3162 c         3586 g         1536 t
ORIGIN      
        1 ctcgtggacc acgtggtgtc gacgttcgcg gagttcgcgg ggatcccggg gctcgtcaag
       61 gacgcgaagc cgcacgtggg gaccgaccgg ctccggcact tctgccggcg gatgcgcgcg
      121 gagctcgcct ccctgggcgc cgtggtccgg ttcggtgcgc gggtcgagga gttatcggtg
      181 tcggaggggg aggtacgcgg ggttctcctg gccgggggcg gggaggtgcg atccccggtc
      241 gtcctgctgg cgacgggcca ttcggcgcgc gacacggtgc ggcggctctt cgcgcaaggc
      301 gtcgggatgt cggggaaggc ggtcgccgtc gggttccggg tggagcaccc ccaggcgatg
      361 atcgaccgga tccagtacgg gccgatggcg gggaaaggcc ttccccccgc ggagtatcgt
      421 cttgcggcgc gcgccccctc cggccgcggt gtctactcct tctgcatgtg ccccggggga
      481 gaggtgatga acgccgcgtc gggggaggga cagtcgcccg tgaacggaat gagcgtccgg
      541 gcccgcaact ccgggttcgg gaacagcggg atcgtggcgt cgatcgcgtc cgcggagttc
      601 ggggaggcgg ggccgcggga aggcggcccc ctggcgggga tcggtttcca ggaggagatc
      661 gaacaccggg cgttcgatcg cggcggcggc gggtacggcg tgccggccgc gaacctgctt
      721 gccttcgtcc gggggaaaga gctccccccg tcccccggac gcctccttgc cccgcgggtc
      781 gtcccggacg atttgcgggg catcctgccg ggtgcggtgg aggaggacat ccggttcggg
      841 ctgctccggt tcgggggggc gatgcggggg tttctcaccc gcgaagcgac gctgtacgcc
      901 gtcgagtccc ggacctcttc gcccgtccgc atcgagaggg tgaattacga gtcggtgacg
      961 cacaaagggt tgtatcctgt cggggagggc gcgggacacg ccggcgggat cgtctcctcc
     1021 gccgtggacg ggatccgcgc cgccctccgc atcctcgaaa cctattcctc gtagaaagca
     1081 ggggtgccga tgcagaagac gcgtttcgtg aaggacctca aggaggggga gccggtccgc
     1141 gaccttttcc tcgtcgcgaa caaggcgctg ctgaccagca acgccgggaa gccgtacctg
     1201 acccttcagc tgcgcgatcg gacgggacag atcgaggcga gggtgtggga ccgcgccgaa
     1261 gagatcggga agcggttcga ccgggacgac gtggtcgagg tgggcggcac cgccatcgcg
     1321 taccaggggc gcgtccagct caaggtccac gacgtccggc gggaggaagg ggggacgaag
     1381 gacctctccg agtaccttcc ggtcacaaag aaggggatcg agccgctctg gaagacgctc
     1441 caggagtaca tcgggggagt caaggacaag gacctggcgc ggcttctcgc ggccgtcttt
     1501 ccggacccgc cggaaacgga cgtcgcgcgc cgcttccgcc aggcgccggg cgggaagacg
     1561 atgcaccacg actacatcgg ggggctgctg gagcacacgg tctccgtcgc ggggatctgc
     1621 cgcatgctgt cggcccatta cgaaggggtg gacgccgacc tgctcctcgc cggcgcgctg
     1681 ctgcatgacg tcggaaaggt gcacgaactt tcctacgagg gggcgttcga ctacacggac
     1741 gaggggcgcc tgctggggca cctctacatg ggtaccgagt acgtgagccg tgtctgcgcc
     1801 gacctccctg gctttccgcc ggagaagacc atgctggtga agcatatcat cctgtcccac
     1861 catggggagc tcgagtacgg ttcccccaag cggcccaaga cgctggaggc gatcctcctc
     1921 catcacgtgg agaacatgga cgccaaggcg acagcgttcg gcgacgccat cgcggaattg
     1981 cgcgaggggg tccgctggac cgactaccag cggatgttcg agcggtacct cttctccggg
     2041 aaatttcccc agggggggtc ttcttgatct gttgggagat ccggggaaga gtgtcccccg
     2101 cttcggaagg gccatgatgg aggcctggaa ggcttttctc gaggagcggg agcggcggca
     2161 gctgctgcgg cggctcgtcc cgtcgtccgg gcgcgccccc gccctcgcgg tgcgggacgg
     2221 cagggaatac gtcgacttct cctcgaacga ctacctcggg ttgtcgtccc atcccgcgct
     2281 ggtcgcggcg gcgcgggaag cactcgaccg ctacggggtc gggtccgggg cgtcccgcct
     2341 gatgagcggg gacctggaga tccaccacga gctcgaagac agcgtggcgg tgttcaaggg
     2401 gagcgaggcg gccctggtct tcaactccgg ctaccaggcg aacacgggga tcattcccgc
     2461 catgttcggg cgccgggacg ccgttttcgc cgaccagttc tgccacgcca gccagttgga
     2521 cggcgccctc ctgtcgcgcg cgaggctgct gcgcttccgg cacaacgacc cggatcacct
     2581 cgaccggatg ctcgggaagc accgcggggc gttcgagcgg gcgctggtga cgacggagag
     2641 cgtcttcagc atggacgggg acatagcccc cctctccgcg ctccttgccg tgtgccgccg
     2701 gcaccgttgc ctgctgatgg tggacgaagc ccacgccacg ggcgtgttcg gcccgcaggg
     2761 gcgcgggtgc gtcgaggcgg aagggctcgc cgggcaggtg gacctcgtga tggggacgtt
     2821 cagcaaggcg ttgggcgggt tcggggcgta tctcgcggcg tcgcggacgg tgatcgacta
     2881 tctggtcaac acggcccgca gcttcatcta ttccaccgcg cttccgcccc cggtgatcgc
     2941 cgccgacctc gccgccctcc ggctttgcct gtccggggag acgcgcgggg aggagcttct
     3001 gcgccgggcc ggcgcgttcc ggggcgcgct tcgcgggaag gggtggaccg tcggcgggga
     3061 gagccagatc gtccccgtcg tcgtggggga gagcgccctc gccgtctccc tctcgataac
     3121 gcttgcgggc caggggttcc tcgcgctgcc ggtgcgcccg cccacggtcc ccgaagggtc
     3181 ggcccgcctg cgtttctcgc tgacgaccgc acacaccgac cggcaaatcg ccgccgtcgt
     3241 ggaggccctc ggtgccgcgt gaggaattca ccgtggcagg ggagggggat ccgtcgatgg
     3301 tcctgctgcc ggggtgggcg acggacgggc ggatcttcga aggggtcctc cccggcgtgg
     3361 ccgcggtgac gaccggtcca ctccggccgg gggggttttc gcggcgcctc gcggtgttcc
     3421 tcgaccggat ggcgtgcggc cccgtgacgg tcgtcgggtg gtcgctcggc gggttcctgg
     3481 cggcggagtt cgcgcgggaa tacccggacc gggtgcggcg ggtcgtgctc gtcgggatcc
     3541 ggcggaagta tccggaagga gacgtggagg ctgtcctgcg ctcgctctcg gccgacccgg
     3601 gcaactgtct ttcggggttc tacgcgcaat gcttttaccc gtcgcagatg agcgcgtacc
     3661 gccggttccg cggcgggctg caggcggcgt acctgaggga gatggacggc ggcgcgctcc
     3721 gcgaagggct ttcgtacctg gagggtgcgc ggctctccgg cgcgacgctt cccgcgtgcc
     3781 ccgtggtgat cgtccatgga gagaaggatg tggtggcgcc gatcgcggag gcggaaggcg
     3841 tggcccggga aagcggaaac gccacgtttc accggctgcc gggggcggcg cacgccgcgt
     3901 tcctcgcgga cgggtttcgc gcggtggccg ccggtggttg accccgacgt cctgcgccgc
     3961 ttctcggccg gcgcggcccg ctacgatgcg cacgcgcacg tgcagcggct ctccgccgtc
     4021 gacctgctgg cgtacaccga ggcgtcgctg gggccgtccc ccaacggggg cctggcgcgg
     4081 accatgaaca ttctcgagcc gggctgcggg acggggctct acacgcggat gctcctcgac
     4141 gccttccccg cggcgtccat cttcggtatc gacgtttccg aagcgatgat acgcgttgcg
     4201 cgcaagcgga tcgacgacca gggatggtct agtgccgcga caggcaggga tgccccgggt
     4261 cttgtcgtcg cggccggcga cccgcgggcc cgcttcgccg tggccgacgc ggaggagatc
     4321 gacgcgggga gctacgacct cgtcacctcc aacgccgtct tccaatggtt tctctccctt
     4381 ccccggacgc tggggcggat ggcgtccctc ctccggggcg gcgggctgct caccttctcc
     4441 ttcttcggcc cggagaccta cgcggagctc gccgacgcgc tccgggcggc cgccggcggc
     4501 aatggaaggc ctggtgccgc ggcaggcagg gatgccccgg ggtttgtagc cgcggccgcg
     4561 ttccactccc gggaagagat ctccggcgcg ctttcggccg cgttcccgcg gtgggaagtc
     4621 gtcgagcgcc ggtaccatca ggaatttccg accctggcgg ccctgttgcg gagcatccgg
     4681 tacacgggga cacgcggggg cggggcgcgg gaggcgtgga gcccggggaa gctcgcgcgg
     4741 gtcgaggagg cgtaccgggg gcgggacggc gggatcaggg cgacgtacca ggtcttcctt
     4801 tgccggggcg tgattccggg aggggagacc gggtgaaggg ggtcttcatc accggtaccg
     4861 gcaccggggt cgggaagacg gtggtgtgcg gcctgctggc cggcttcctc cgggcgcggg
     4921 ggatgcgcgt gacgacgcag aagtgggtcg agacgggcgt gacggacggt ccgtcggaca
     4981 tcgatgtcca ccgccgcctg atgggaggcc ccggtcccgc gccggaaccg ccgccggcgg
     5041 accggtgccc gtaccggttt tccctccccg cttccccgca ccttgctgcg gctcgcgagg
     5101 ggcggcgggt ggccccggtc gtgatcgagg cggcgtaccg ccgtctcgcg gagagccacg
     5161 acgccgtgct ggtcgagggg gcgggcggct tcctcgtacc tctctcggag gaactgttga
     5221 ccggcgacct cgtcgcgcgg atcggccttc ccgtcctcgt ggtggcgggg aaccggctcg
     5281 gctgtgtgaa cgacgcgctg ctcacggtgg aggcggtgcg ccgacgcggg atctctctgc
     5341 aggggatcgt cttcaaccgc cttccgggcg aaggggacgg gacgcgggcg gaggtgctcg
     5401 ccgacaatcc ccggatcgtg gcggagatca caagggcgcc cgtcctgggc gaggttcccc
     5461 tgctgcccga cccggcccgc ggcgcggagg cattcgcccc cgtcgggcgg gcgtttctcg
     5521 aacggtggag gtcgacctga tggaccggaa aagctggctc cggaaggacc tgcggcacat
     5581 ctggcatccg tacacccaga tgagcaccct tgcgacggag ccgccgatgc tgatcgatcg
     5641 cgcggagggg ctcttcctct tcgacgcgga tgggaaccgg tactacgatg cgatctcaag
     5701 ctggtggtgc atcgtccacg ggcacgggca cccgaagatc cgggaggcgg tgacgcggca
     5761 gatggagcgg ctggaccacg tcctgttcgc cggcgtcacc cacgagcccg cggtgcgcct
     5821 ggcctcgcgg ctcgccgcga tcgccccgga ggggctttcg cgcgtcttct tctcggacaa
     5881 cggttccacg gcggtggagg tggcgctcaa gatgtcgctg cagtgctgga gcaatctcgg
     5941 gcgggaggag cggaccgggt tcgtctgcct cgatcacggg tatcacgggg acaccaccgg
     6001 gtgcatgagc gtcagcggcg tggaagcgtt cctgcgcgcg ttccggccga tcctgttccc
     6061 agcccgccgg gttcccgcgc cgtactgcta ccgctgcccg gtggggaaga cgtacccgga
     6121 gtgcggggtc tcttgcgtcg acgtcctcgg cgacgcgctg cgggaaggcg gcgaaacgat
     6181 cgcggcggtg atcctcgagc cgctgctgct tggcgcgggc gggatgatcg tctatccgcc
     6241 ggaatacctt tcccgcgcgg cggctctcgc gcgcaagcac ggcgcccact ttatcctcga
     6301 cgaggtggcg acggggttcg ggcgaacggg gacgatgttc gcctgcgagc aggccggtgt
     6361 ctcaccggat atcctctgcc tgtcgaaggg attgacgggc ggcacgatgc ccctggcagc
     6421 gacgctcacc accgcggagg tgtacaactc ctttctcggg ggtccggaca gcgggaagac
     6481 gttctaccac ggccacacgt acacggcgaa cccggtcggc tgcgccgccg cgctcgcctc
     6541 cctcgacctg ttcgaggaag aagagctcgt cgaccgggtg gcgcgcctcg cgccccgcct
     6601 cgcggaaggg gtgcgggagc tcgccgggct gccgttggtg ggggacgtgc gcgggatcgg
     6661 gatggtggcg gcgctcgagc tggttcggga caagggaacg aaggagccgc tgccggggac
     6721 tgcgccgctg ttccgcgata tccgccgcga ggggctggac cgcgggctct tcctgcgccc
     6781 tctcgggaac gtggtctacc tgtttctccc ccaggcggtc acccgcgaag ccctcgacga
     6841 catcctcgac cggttcgggg agacgctgcg gaaagtgctg tcatgacccg ggagatgggg
     6901 gcggcgagag gatgggtccg ggattgccgc tccctgtgtg tgctgaccgg cgccggggtg
     6961 tcggcggaga gcggcgtacc cacgttccga gggcccgaag ggttgtggaa gatgcgggac
     7021 ccgacgactc tcgccacccc ggaggcgttc gcggccgatc ctggggaggt gtgggagtgg
     7081 taccagtggc gcaggcaaag gatccggggc tgcgcgccga acccgggcca cacggccctc
     7141 tcggccgtcg aagcggcaat ccccgacttc ctcctcgtca cgcagaacgt cgatgggctt
     7201 caccgggcgg ccggttctcg tcgggtggcg gagatccacg ggagcatctg ggtcgtgcgg
     7261 tgcacggggt gcggtgcgga gcgcgaggag cgggacgatt tcgcggagct tccgccgcgg
     7321 tgcgccgcgt gcggcgggat gctgcgcccc ggggtggtct ggttcgggga gacgcttccc
     7381 accggcctgt ccgacgcggt gatggagatg ctctcccgtt gcgaggtgct gatcgtcgcg
     7441 gggacctctg cggtcgtctc ccccgcgtcc ggctacgccg ccgtggcgaa gcggcgcggc
     7501 gcccgggtga tcgaagtgaa cccggacgag acgcccgtgt ccgcgctctg cgatgcgacg
     7561 ttccgcggga aatcgggcga ggtgctgccg gcgctgttcg gtcaccccaa atcgtcgtaa
     7621 gggcgaaggc ggaaggagac gccggggatc gactcggcga gccgtcggac cgcctcgggg
     7681 tcgaggccgg gcagggcgac ggcggtcgcg ctcacctttg gtacgtgctt cggggcctcc
     7741 cggaggaagt cgagcagggc ggcgaacgat gccgccccgt accggttcgg gcagatccgg
     7801 gcgtacgtct gcgcgtccgg cgcgttcagg gatacggaga gggcgtctac cagccccgcg
     7861 atctccggca ggatgttccg gccatggacg agatttccca gcccgtccgt gttgacccgc
     7921 acgcgcgccc cgcgccgctt cagttccgcc gcgatctcct tcatcacgtc caggcgcagc
     7981 agcggctccc cgaagccgca gaagacgact tcgtcgaact tcgtcgggtc cccgatctct
     8041 gcgagcgcct ccgcagcggt cggctcgccc ggcaatttca gaaggtgccc cttcacgtgg
     8101 tagtcgtgcc gcttcgggca gaaggtgcag gcgttcgtgc accggttcgt gatgttcagg
     8161 tagagggagt cgcggatctt gtacgtgatc cggacctcct cctcgacggg gatgcggaag
     8221 aggcgcaggg cgttgatcgt ggtcgtccgc gccacgtcct cgacggtgac ccccttgatc
     8281 gccgcgatct tcgccgccac cagcggcaca tacgacggtt cgttcggctt cccgcggtgc
     8341 gggatcggcg ccaggaaggg gcagtccgtc tcgatcagca ggcgatccag cggcagctcg
     8401 cgcaccgcct cgacctgctt ctccgacccc ttgtaggtga tggccccggg gatggaaacg
     8461 aggaagttca tcccgagcgc cctgcgcgcc atcgcgaggt ccccggagaa gcagtggatg
     8521 atgccgccta cctccgcggc gttctcctcc gaaaggatcg acagcacctc gtcgtgcgcg
     8581 tcccggtcgt ggatgaccac ggggaggccg cgccggcggg ccaggcggat ctgttcgcgg
     8641 aaggcggcgc gctggctatc cctgggcgcg cggtcgcgat ggaagtcgag ccccgtttcc
     8701 ccgatcgcca ccaccttgtc gcaccgggag agcgcctcga ggcgggcgag gagcttgtcg
     8761 gagagtcgcc ccgcgtcgtg cgggtgcagg ccgacgacgg cgtaaacccc cgtgtgccgg
     8821 tgggcgatcg atacggcccg ctcgccgctc tccgggtcga tgccgaccgt ggcgatccgg
     8881 gtgacccccg cgtcgcgcgc ccggcgcacc acctcctcct ccgcgtcgta gagcggcgaa
     8941 aggtcgaggt gcgcgtgggt gtcgaagaac atctggttgg gccgagggcc tacggacgac
     9001 tcaaacttcg atcttcggga aaacgatgca cgcgttcggg agggtggcgc ccgccgcgag
     9061 ccctccccac gcgccgtccc ggtcgatccg tacgttttcc acggctccgc cctcgggaac
     9121 gagggcttcc cacagtttct gcgccgcggt cggggtgaac ggggccatca tcagagcggc
     9181 aacccgggac gcctcgaggg cgttgtagag aaccgtcccc agccgggcac gcttccccgg
     9241 gtccttcgcc agcgcccacg gctgcgccga ctgcacgtat tcgttcccct tcgtcacgag
     9301 ctcgatgatc gccccgagcg ccttgtgaaa cgccacctcg tccatcgccg cagccacggc
     9361 cggcaagctc ttgcgcccgc aggcgaacag gaccgcgtcc tcgggttcgg cgggccccgg
     9421 ctcggggacg acgccggcga aatatttccc gagcatcccg agcgatcggt tcagcaggtt
     9481 ccccagcttg tcggcgagtt ccgagttggc gcgcttgatc agctccttct ccgagaagtc
     9541 gccgtccagc ccgaaggaga cctcgcgcag caggaagtat cggaacgcgt ccgccccg
//
LOCUS       DASVJL010000355         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1371831, whole genome shotgun sequence.
ACCESSION   DASVJL010000355 DASVJL010000000
VERSION     DASVJL010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371831"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VF357_07505"
     CDS             complement(<1..285)
                     /locus_tag="VF357_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524032.1"
                     /translation="MGLKAYMNDIIEAVVTTAKGMRITARYGVDPREEVTLQYPEERW
                     EIPQGYRGFLHNDIKTCTACTMCVKTCPVDCISLESVRGADKKMVLASYDI"
     gene            complement(290..1405)
                     /gene="nuoH"
                     /locus_tag="VF357_07510"
     CDS             complement(290..1405)
                     /gene="nuoH"
                     /locus_tag="VF357_07510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX7524033.1"
                     /translation="MEALALSLVNAVPLLAGVPLWAVTLLLMVVVAAVILVFALTFEG
                     VGSLVLRKVAGDIQARIGPNRVGPNGMLQFLADGVKLVLKEDIIPANADRLLFKLAPY
                     FVFVGSFAAFVVVPFGVGLIAADLNIGIYYVMAVTSLVVIGVLLAGWASNNKWALLGG
                     MRAAAQIVSYEVPVGMALLPAVLIAGSLSLQDIVHSQGGLLGIFGWNLFHNPFTFFSF
                     FLYFTAAQAETNQTPFDLPEAESELVSGYNVEYSGIRFGLFFLAEFGDMFIVAALSTA
                     CFLGGWNVPFFNAGTLPWIWGNLFSLGVFLTKAFTMVLIMMWVRWTLPRLRVDQLMRM
                     AWKYLVPLTFVNLLGVSLWLLVFKGKGIPGMIASWLG"
     gene            complement(1407..2537)
                     /locus_tag="VF357_07515"
     CDS             complement(1407..2537)
                     /locus_tag="VF357_07515"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX7524034.1"
                     /translation="MTARTDALPTQEMTINMGPQHPSTHGVLRVILTTDGEVVTRAVP
                     DVGYLHRALEKIGERVTYAQYMPFTDRLDYLAAMNCNCAWAWTVEKLAKIEVPERAEY
                     LRVIVCELNRISSHLIAFGSYTADMGAFTPFLYAIRERERVNDLFEMICGNRLTYNYA
                     RIGGVSGDAPPGFLEKTKEFLDYFEPKLDEYNNLISYNKIFVHRLANVAVIPAEEAVA
                     YGLTGPNLRGSGVHFDLRKDEPYSVYPKFDFKVCVGTGERGTLGDCFDRYMVRINEMR
                     ESIKIVRQAVAQIPDGPVLAKVPRLFKPPVGEVYFRSECPRGETGIYLISDGSVNPFR
                     LKIRTGSFVTMGVFEKVTRGLMIADVVAVIGSFDIILPEIDR"
BASE COUNT          484 a          850 c          864 g          425 t
ORIGIN      
        1 gatgtcgtag gaggcgagga ccatcttctt gtccgccccc cgcaccgact cgagggagat
       61 gcagtcgacg gggcacgttt tcacgcacat cgtgcaggcg gtgcacgtct tgatgtcgtt
      121 gtggaggaag ccgcggtacc cctggggaat ttcccaccgc tcctcggggt attgcagcgt
      181 gacctcttcc ctcgggtcca cgccgtagcg cgcggtgatc cgcatcccct tcgccgtggt
      241 gaccacggcc tcgatgatgt cgttcatata tgccttcagg cccatggtct catccaagcc
      301 acgaggcgat catcccgggg atccccttgc ccttgaagac caggagccag agggagacgc
      361 cgaggaggtt gacgaacgtc agcggcacca ggtatttcca cgccatccgc atcaactggt
      421 ccacgcgcag ccggggcagc gtccagcgga cccacatcat gatcagcacc atcgtgaacg
      481 ccttcgtgag gaagacgccg agggagaaca ggttccccca gatccacgga agcgtcccgg
      541 cgttgaagaa cgggacgttc cacccgccca ggaagcaggc ggtggacagc gccgccacga
      601 tgaacatgtc gccgaattcc gccaggaaaa agaggccgaa gcggatcccg gagtattcca
      661 cgttgtagcc ggaaaccagc tccgactcgg cctcggggag gtcgaacggg gtctggttcg
      721 tctcggcctg cgccgccgtg aagtagagga agaaggagaa gaaggtgaag gggttgtgga
      781 acaggttcca cccgaagatc ccgagcagcc ccccctggga atggacgatg tcctgcagcg
      841 acagcgagcc ggcgatgagc accgcgggga ggagggccat ccccaccggg acctcgtagg
      901 agacgatctg cgccgcggcc cgcatgccgc cgaggagcgc ccacttgttg ttggaggccc
      961 accccgcgag caggaccccg atcaccacca gggaggtgac cgccatcacg tagtagatgc
     1021 cgatattcag gtcggcggcg atcagcccca cgccgaaggg gacgaccacg aaggcggcga
     1081 aggagccgac gaagacgaag tacggcgcca gcttgaagag gaggcggtcc gcgttcgccg
     1141 ggatgatgtc ctccttgagg accagcttca cgccgtcggc caggaactgg agcatcccgt
     1201 tgggcccgac ccggttcggg ccgatccggg cctggatgtc gccggcgacc ttgcgcagca
     1261 ccagcgaccc caccccctcg aacgtcaggg cgaacacgag gatgaccgcc gccaccacca
     1321 ccatcagcaa cagggtcacc gcccacaggg ggacgcccgc cagcagcggg acggcgttta
     1381 ccagggaaag ggccagcgct tccatcttat ctgtcgatct ccggtaggat gatgtcgaag
     1441 ctgccgatga cggcgacgac gtcggcgatc atcagtcccc tcgtgacctt ctcgaagacg
     1501 cccatcgtca cgaaggagcc ggtgcggatc ttcagccgga aggggttcac cgagccgtcg
     1561 ctgatcaggt agatgcccgt ctccccgcgc gggcactcgg agcggaagta cacctcgccg
     1621 acgggcggct tgaagaggcg gggcaccttc gccagcaccg gtccgtcggg gatctgcgcg
     1681 accgcctgcc gaacgatctt gatgctctcg cgcatctcgt tgatccgcac catgtaccgg
     1741 tcgaagcagt cgccgagcgt gccgcgctcc ccggtgccga cgcagacctt gaaatcgaac
     1801 ttcgggtaga cggagtacgg ctcgtccttc cgcaggtcga agtggacgcc ggatccgcgc
     1861 aggttggggc ccgtcagccc gtaggcgacc gcctcctccg cggggatcac ggcgacgttc
     1921 gccaggcgat ggacgaagat cttgttgtag gagatgaggt tgttgtattc gtcgagcttc
     1981 ggctcgaagt agtcgaggaa ctccttcgtc ttctcgagaa agcccggagg cgcgtcgccg
     2041 gagacgccgc cgatgcgggc gtagttgtac gtcaggcgat tcccgcagat catctcgaac
     2101 aggtcgttga cccgctcccg ctcgcggatg gcgtacagga acggagtgaa cgcccccatg
     2161 tccgcggtgt acgacccgaa cgcgatcagg tgggaggaga tccggttcag ctcgcagacg
     2221 atgacgcgca gatactcggc gcgctcgggg acctcgatct tcgccagctt ctccaccgtc
     2281 cacgcccagg cgcagttgca gttcatcgcc gccaggtagt cgagccggtc cgtgaacggc
     2341 atgtactggg cgtaggtgac gcgctccccg atcttctcca gggcccggtg caggtacccg
     2401 acgtcgggca ccgcgcgggt caccacctcg ccgtcggtgg tgaggatcac ccggagcacc
     2461 ccgtgggtgc tcgggtgctg cggccccatg ttgatcgtca tctcctgcgt ggggagcgcg
     2521 tcggtccggg ccgtcatacc ttgatcccgt ggtagaagtc ggggtacttg taatccttcc
     2581 gcagcgggtg cccctcccag tcctccggga gaaggatgcg gcg
//
LOCUS       DASVJL010000356         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_340453, whole genome shotgun sequence.
ACCESSION   DASVJL010000356 DASVJL010000000
VERSION     DASVJL010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340453"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VF357_07520"
     CDS             complement(<1..486)
                     /locus_tag="VF357_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEX7524035.1"
                     /translation="MYKRKFASDAKVSKFAFPSFEGIAEPVRTGRPEERGARPSVKTP
                     EDVEREAHIRGFEAGEKAGAESVSREAASLLEGLRCAADGVRVLREQIAREAEPQVVE
                     LAVAIARRIVMEELSASPGRIVAIVKEAIRRIERAGPVTIKVHPDLYNLMAGVKAELA
                     ES"
     gene            complement(479..1474)
                     /gene="fliG"
                     /locus_tag="VF357_07525"
     CDS             complement(479..1474)
                     /gene="fliG"
                     /locus_tag="VF357_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX7524036.1"
                     /translation="MAANVLSGYEKSAIFLSLVGEEAAAEVLKNLDMQEVGKITTQMS
                     RLKSIRKEMMDVVLKEVTGIISQDDVQFVSGNEYIKKVLNKGFGADSASKVLDRALKD
                     APLDSLRWVDPWTLANFLMTEHPQTVAFILCLLEPARAAEILAFFNETLRADVAMRIA
                     TTERIPETAIEEIRSALDGQLELGAGSGIKFGGMKTIAEILNRSDRSMEAAMFKKIEE
                     QDKPLAESIRELMFVFDDLVELDDRSMQMVLKEVSTDELALALKTAAEELRTKIFKNM
                     SQRAGEILKDEIATKGPVKVTDVEKSQQKIVGIARKLEAEGKIVIGGRGGEEIVV"
     gene            complement(1475..>2445)
                     /gene="fliF"
                     /locus_tag="VF357_07530"
     CDS             complement(1475..>2445)
                     /gene="fliF"
                     /locus_tag="VF357_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX7524037.1"
                     /translation="HLVASSVEDLNSQEITVVDAKGNILSKPGGDVAGLSASQFDYQS
                     GYSKDLEARIISILEPVVGKGKVRAKVSAVVDFTRSETTEEKFDPESQVVRSEQKQNE
                     KSTTVGAGGTPGVASNLPGKTTVAATPSQGLSEKQSQTVNYEMTKITSRTVNAPGLVK
                     KLTAAVLVDGTYAPQQGSKEMKYTPRSEEDVRRYEELVRETMGFTESRGDQIKVVNMP
                     FEVAREEEVLPPKTKILEMAAPAARYALPLLAVILLVVFVLRPVAQSLSASGSNFPRG
                     PVPIAQAFAEIENAGVRKELPARAQVADWARKNPTDAANVIKSWMQ"
BASE COUNT          368 a          838 c          728 g          511 t
ORIGIN      
        1 ggactccgcg agctcggctt tcactccggc catcaggttg tagaggtcgg ggtgcacctt
       61 gatggtgacc gggccggccc gctcgatcct ccggatcgct tccttcacga tcgcgacgat
      121 ccgcccgggg gatgcggaaa gctcctccat cacgatccgc cgggcgatgg ccacggcgag
      181 ttcgaccacc tggggctcgg cctcccgggc gatctgttcc ctcaggacgc gcactccgtc
      241 ggcagcgcac cgcagcccct cgaggagcga ggcggcctcg cgcgagacgc tttcggctcc
      301 agccttttcc cccgcctcga acccccggat gtgcgcctcc cgctcgacgt cctcgggcgt
      361 ctttacggag ggccgggctc cccgctcttc cgggcgcccg gtgcggacgg gttcggcaat
      421 accctcgaac gaggggaagg cgaatttcga aaccttcgcg tcgctcgcga acttccgctt
      481 atacaacgat ttcctctccg ccccggccgc cgatgacgat cttcccctcc gcctcgagct
      541 tccgggcaat gccgacgatt ttctgctgcg acttctcgac gtcggtgacc ttgaccggtc
      601 ccttcgtggc aatctcgtcc ttgaggatct cgccggcccg ttgcgacatg ttcttgaaga
      661 tcttggtcct gagctcctcg gcggccgtct tcagcgcgag cgcgagttca tcggtgctca
      721 cctctttgag gaccatctgc atggaacggt cgtccagttc gacgagatcg tcgaacacga
      781 acatgagctc gcggatcgac tccgcgagcg gcttgtcctg ctcctcgatc ttcttgaaca
      841 tcgccgcctc catcgaccgg tcggaccggt tgaggatctc ggcgatcgtc ttcatcccgc
      901 cgaacttgat accggagccc gccccgagtt caagctgccc atcgagagcg ctccggattt
      961 cttcgatggc ggtctcgggg atcctctccg tcgttgcgat ccgcatcgcg acgtcggccc
     1021 gcaatgtttc gttgaagaac gcgagaattt ccgccgcgcg ggccggctcg agcaggcaga
     1081 ggatgaaagc gaccgtctgg gggtgctcgg tcatcagaaa attcgcgagt gtccacgggt
     1141 cgacccaccg gagcgagtcg agcggggcat ccttgagggc gcgatcgagg accttggagg
     1201 cgctgtccgc accgaatccc ttgttcaaga ccttcttgat gtactcgttt ccgctgacaa
     1261 actggacgtc gtcctgagag atgatccccg tgacctcctt gagcaccaca tccatcatct
     1321 ccttccggat cgacttgagg cggctcatct gggtcgtgat cttgcccacc tcctgcatgt
     1381 ccaggttctt gaggacctcc gcggcagcct cctctcctac gaggctcagg aagatcgcgg
     1441 acttttcgta tccgctcagc acgttggccg ccatttactg catccagctc ttgatgacgt
     1501 tcgcggcgtc cgtcgggttc ttgcgcgccc agtcggcgac ctgcgcccgg gccggcaatt
     1561 ccttgcgcac gccggcattc tcaatctcgg cgaaggcctg cgcgatcggc accggccccc
     1621 gggggaagtt cgatccggag gccgacagcg actgtgcaac gggcctcagc acgaaaacca
     1681 ccagcaggat gaccgcgaga aggggcaggg cataccgcgc agcgggggcg gccatttcga
     1741 ggatcttggt cttgggcgga agaacctcct cctcgcgggc gacctcgaac ggcatgttca
     1801 cgaccttgat ctgatcgccc cgactctccg tgaagcccat cgtctccctg acaagttcct
     1861 cgtatcggcg aacatcctct tcgctgcggg gggtgtactt catctccttg gagccctgct
     1921 ggggggcgta ggtcccgtcg accaggaccg ccgccgtgag tttcttgacg aggccgggag
     1981 cgttgacggt acggctcgtg atcttcgtca tctcgtagtt caccgtctgg ctctgcttct
     2041 cggacagtcc ctgcgacggc gtcgcggcga ccgtcgtctt ccccgggaga ttcgaggcca
     2101 cgcccggcgt gccgccggcg cccacggtcg tcgacttttc gttctgcttc tgttcgctcc
     2161 ggacgacctg gctttccgga tcgaacttct cctccgtcgt ctcgctccgc gtaaagtcga
     2221 ccaccgcgga gactttcgcc cgaaccttcc ccttgcccac gaccggttcg aggatactga
     2281 tgatccgcgc ctcgagatcc ttcgagtagc cgctctggta gtcgaactgg ctcgcgctca
     2341 gacccgcgac atcgccgccg ggcttcgaga ggatgttccc cttcgcgtcg acgacggtga
     2401 tctcctgcga gttgagatcc tcgacgctgc tcgcgacgag gtgga
//
LOCUS       DASVJL010000357         9074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_247386, whole genome shotgun sequence.
ACCESSION   DASVJL010000357 DASVJL010000000
VERSION     DASVJL010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247386"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VF357_07535"
     CDS             complement(<1..799)
                     /locus_tag="VF357_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX7524038.1"
                     /translation="MNRLLTALIALAVLAAIPAVSLADATHVVRKGDTLGKIARAHHV
                     SVAKIKAANGIEGNHLSVGTKLVLPGGKVDSKRRKARSGKGSRGTVRTSTPAEAPTPG
                     AQLSLVDPQESTLHTGTWKPPTEAELAELARAPKTGDTASPTVAASPTVAAAKDASKA
                     DESIKDRLLRVAQRMLAVPYRFGGTTLWGLDCSGFVQKAFAFLDLGLPRTAREQFREG
                     LKISKADLAAGDLVFFRTYAKYPSHVGIYLGDNRFIHASSRDRKVTID"
     gene            947..2155
                     /locus_tag="VF357_07540"
     CDS             947..2155
                     /locus_tag="VF357_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7524039.1"
                     /translation="MTITSGSRLRHRQFSIGPVALFLYLFSSVFPVAPCVAADHPDTL
                     KIGVLAPLSGPYTAGGTSFVQAATLAVEQANAEGGVFGQRVTIVVGDTQGRVDVAKAE
                     ALRLVSREKVSALVGAYLSEETVGVMEVAAAQRMVLLVPVAATAEITDKVRREYARYR
                     YVFRVGYSLPQWAEMIAAFLSDRKVQRYAFIGAGIRWNLELGETLERIVASRGITQAY
                     TAFYSPGNPAFDTVAVAAAAVSPDIVILADPGRNSVSFVKRLRESAPTLPVLSIGGAL
                     GDARLADAVPLSAPFYVQAAAWRGVSPAATAYVERYEHRYGAPPVGYSDTLPYDAVTV
                     LVAAWRKAGSAATEAVVPVLERGAFKGAAGTYRFDATHQAQWGTGPEALHGTFVRWER
                     GGARIVFPPP"
     gene            2181..3023
                     /locus_tag="VF357_07545"
     CDS             2181..3023
                     /locus_tag="VF357_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7524040.1"
                     /translation="MLLDGLFAGGTYALMAAGMALILGVVKVINLSHGAFFTLGAYVA
                     YALAQRGVGTPIAAASLAAVIAFAFGVFLGKSFVNPVRSHPFALPVGTLACALLFEQA
                     AQMIWGPHPLLIEAGTPWIGPAGLVIRRWGVIAFLSSAALLGGLKWVLSSRPGLPLRL
                     IAEDEEIAGSLGMDVDAIRYLTFGGACAMAAAAGALLSPSGAVTPTMGRAPLLLSLVV
                     VIVAGMDSVAAIFLLSIGLGIFGNACAWLLSPQWSYVALLAAVCLLLSARPAGIVVLA
                     GRRD"
     gene            3020..4033
                     /locus_tag="VF357_07550"
     CDS             3020..4033
                     /locus_tag="VF357_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7524041.1"
                     /translation="MRNAASFRGGWLAAGIGLLLLFPLSSPDAFFLDVFTTGFLLAAF
                     AASWDVVGGVSGQITLGHALPFGTAAYACALLTSFAGWPLPLAVAAAVLLAAAVGAGI
                     GTLSAPLAGPFVALLTLALGEIAHEVAVGHTFFSGPGGYAWGGEGGIPVALPWRDASP
                     FSSYYAALAFLCIASFGMLRFSRSGAGLILRAVAGSPITAQASGVPIARFKRLAFAVG
                     SAVAGAAGAGFVLHVGRATATDLSLELSFQAATFSAVGGLGTIVGPVVAALVLHVLFQ
                     GLSLPPSARVLLYALTLLVTLRFFPGGVVGTARDLASRLRGREGTRPPRRRGRNRAGE
                     GTP"
     gene            4030..4719
                     /locus_tag="VF357_07555"
     CDS             4030..4719
                     /locus_tag="VF357_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7524042.1"
                     /translation="MNTLLSAEGITKAFGATQVLSGITLSVAPGEPVGLFGPNGSGKT
                     TLVNILSGLLLPDAGTVRFEGKEITRLPLDARYRLGIARTFQIPHPYPALSVREAVRV
                     ALAANAGKRKEEGRTREDEEGPAEDLLVRTGLFNQRFVPCAQLSQGCLRRLEFARGLA
                     CRPKLLILDEIFSALSAADEGDLMALLRTANRDEGTALLLVSHNPPLLKALCRRILVL
                     EEGRIVRERAV"
     gene            5013..5792
                     /locus_tag="VF357_07560"
     CDS             5013..5792
                     /locus_tag="VF357_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7524043.1"
                     /translation="MPGGTSTLKVRDLTVAFGQVVAVRDVSLTAREGETVAIFGANGS
                     GKTTFLKAVAGLIQTTRGKVAWRGEDVTALPAHERAARGIRYVSDRSRVGTRMTVLEN
                     LEAGAWLLPSARWSAARERVFSLFPVLAEKARSPAGVLSGGERQMLILGRALVAEPTL
                     LLMDEPFLGLSREIRDRLLSVIEGTLKGRATILLAEHDAEGAYRLLDRHVIFRNGSVV
                     HEGTRSDVADAKELVSLLYRHFRPGEAPGETIGEAERKEKP"
     gene            5789..5974
                     /locus_tag="VF357_07565"
     CDS             5789..5974
                     /locus_tag="VF357_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524044.1"
                     /translation="MRGKTGGEKENGEREPVSVTVTLPSALVDQLKEFCRNCRVTRDI
                     VVERALVEYFREGDMSH"
     gene            complement(6109..7062)
                     /locus_tag="VF357_07570"
     CDS             complement(6109..7062)
                     /locus_tag="VF357_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HEX7524045.1"
                     /translation="MAELLASGGSMDMVRAAFDNGADAVYVGATGWSRRRAQYEMDDA
                     AIVEAARYARSAGRILRVAFNTLPASSEVPLFLVRTDALHDAGVRDFILTDPGLMMAL
                     KNRHPDTILHASVGCTIINVQDALFYKEAGASQVVAECRMGKATMRRIKVEAGVGLEV
                     LVHATTCYTLLGRCTMSSYTKYGHRVDASGKDHFPGSPNRGGLCYRICLSDWDRVGAA
                     GELEESGVELPNRAYFLADDIPDLIDLGVDTIKIQGREYSVPLVGRMVRFYRDLIDAC
                     VRDRAGFRMDPWRERMARIVADRDAERSEKTAGLISESLDR"
     gene            complement(7117..7974)
                     /locus_tag="VF357_07575"
     CDS             complement(7117..7974)
                     /locus_tag="VF357_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX7524046.1"
                     /translation="MCLGSPYCRRVEGNYAEALDLLPGVVSRLHAAGKRAYVSTPAVP
                     READLPHVGRLVDAAAAAGADALEIHNMGVLRIVREKGRQVPAHMGAYANVYTHLAAG
                     VMRDAGAVRVRPNAEVSLEEMAILAREAGVEVEVLVHGKIPLGVTDRCFLLTEPEETD
                     PKCPSACGEVHWLTSRQWVLKTVGKGVLSGRDMCLLEHLPRLVADGFRVFRVEGLYET
                     AAYRSEIGAAYREALARAFAGGSFALEERWADAARRHAPRGLCNGYCFGMAGRTYVGT
                     VLQDANPEV"
     gene            complement(8046..8639)
                     /locus_tag="VF357_07580"
     CDS             complement(8046..8639)
                     /locus_tag="VF357_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HEX7524047.1"
                     /translation="MAFINYSSREINCKIVYYGPGLCGKTTNLQVIHKRMNPETRGKM
                     ISLATETERTLFFDFLPLSLGEIRGFKTRFHLYTVPGQVFYDASRRLILRGVDGVVFC
                     ADSQLTRADSNEESMENLRMNLREQGYDPDRLPLVIQYNKRDLPNVASLAELHALLNR
                     RNVPEFEAAASKGVGVFETLKTVIKLVLIDLKRGGRQ"
     gene            complement(8667..>9074)
                     /locus_tag="VF357_07585"
     CDS             complement(8667..>9074)
                     /locus_tag="VF357_07585"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX7524048.1"
                     /translation="LLVDASAKVVFLVDKDGTLLASAGDAVGFDTTSLASLAAGNIAA
                     TGGLANLIGEKEFSILFHEGERDNMHLSVVAERLILVVVFDRRSSVGLVRLRVRQASA
                     RFTAIMAVAMAANEDEPGGVEELTEADIESLFK"
BASE COUNT         1315 a         3041 c         3201 g         1517 t
ORIGIN      
        1 tgtcgatcgt gaccttccgg tcgcgggacg aggcgtggat gaaccggttg tccccgaggt
       61 agatccccac gtgggagggg tatttcgcgt aggtgcggaa gaagaccagg tcgcccgccg
      121 cgaggtccgc cttcgagatc ttcaacccct cccggaactg ttcgcgggcg gtacgcggaa
      181 ggccgaggtc gaggaaggcg aacgccttct gcacgaatcc ggagcagtcc aatccccaga
      241 gggtggtgcc gccgaaccgg tacgggacag cgagcatccg ctgggcgaca cgcaggagac
      301 ggtccttgat cgactcgtcc gccttcgagg cgtccttcgc tgcggcgacg gtcggggacg
      361 cggcgacggt cggggacgcg gtgtcccccg tcttcggcgc ccgggccagt tcggcgagct
      421 cggcttcggt gggaggcttc cacgtgccgg tgtgcagcgt ggattcttgc gggtcgacga
      481 gggagagttg cgccccgggc gtcggcgctt ccgccggtgt cgacgtgcgc acggtccccc
      541 ggctgccctt cccggagcgg gccttccggc gcttcgaatc caccttgccg ccggggagga
      601 cgagcttcgt ccccaccgag aggtggttcc cctcgatccc gttcgccgcc ttgatcttcg
      661 ccacggagac gtggtgggcg cgcgcgatct tgcccagcgt gtcccctttc cggaccacgt
      721 gcgtggcatc ggcaagggag acggcgggga tcgccgcgag gacggcaagc gcgataaggg
      781 ctgtgagcag ccgattcatg tcagaaatag ttctccatgc gcttcctgat taagtcggca
      841 aagtatacca taggatcctc cgcaggggag aataaaaatc ccctgattta tggtatagtt
      901 gtagccgcat ccctccgccg accctaccgt ttcgggagga cgaaccgtga cgatcacctc
      961 tggatctcgc cttcggcatc gacaattttc catcgggccg gtggccctgt tcctttattt
     1021 attttcgtcc gttttccccg tggccccctg cgtcgcggcg gatcacccgg acaccctgaa
     1081 gatcggggtg ctggccccgc tctccgggcc atacaccgcc ggggggacat cgttcgtgca
     1141 ggccgcgacc ctcgccgtcg agcaggcgaa cgcggagggg ggggtcttcg ggcagcgggt
     1201 gacgatcgtc gtcggcgaca cgcaggggcg ggtggacgtg gccaaggcgg aagcgctgcg
     1261 actggtttcc cgagagaagg tctccgccct ggtgggcgca tacctttcgg aggagaccgt
     1321 gggggtgatg gaggtcgccg ccgcgcagcg gatggtgctt ctcgtccccg tggccgccac
     1381 cgccgagatc accgataagg tgcggaggga gtacgcgcgc taccggtacg tcttccgggt
     1441 gggatactcg ctcccccaat gggcagagat gatcgccgcg ttcctgtcgg accggaaggt
     1501 ccagcggtac gcgttcatcg gcgccgggat ccgttggaac ctggaactgg gggagacgct
     1561 cgagcggatc gtcgcgtcgc gggggatcac gcaggcgtac accgccttct atagcccggg
     1621 gaacccggca ttcgacaccg tcgcggtcgc cgcggcggcc gtctctcccg acatcgtgat
     1681 tctcgccgac cccggacgga actccgtctc cttcgtgaaa cggctgcgcg agtcggcgcc
     1741 cacgctcccg gtgctctcca tcggcggagc gctgggggac gcccggctgg ccgacgccgt
     1801 tccgctctcc gcgccgttct acgtgcaggc cgccgcgtgg cggggcgtct cccccgccgc
     1861 gacggcgtac gtggagcggt acgagcaccg gtacggtgcg ccgcccgtcg ggtacagcga
     1921 cacgcttccc tacgacgccg tcacggtgct cgtggccgcc tggcgcaagg cggggagcgc
     1981 tgcgaccgaa gccgtcgtgc cggtcctcga acgcggagcg ttcaagggcg cggcgggcac
     2041 ctaccggttc gacgccaccc accaggccca gtgggggacc ggccccgagg cgctgcatgg
     2101 gaccttcgtg cgctgggaac gcggcggcgc ccggatcgtc ttccccccgc cctgatccag
     2161 tggagtctcc ccttctccag gtgctcctgg acggcctctt cgccggggga acgtacgcct
     2221 tgatggcggc ggggatggcg ctgatcctcg gcgtggtcaa ggtgatcaat ctttcccacg
     2281 gggccttctt tacgttgggg gcctatgtcg cctacgcgct tgcccagcgg ggggtcggga
     2341 ccccgatcgc cgcggcgtcc ctcgccgccg tcatcgcctt cgccttcggc gtcttcctcg
     2401 gcaagagttt cgtcaacccg gtccggagcc acccgttcgc cctcccggtg gggaccctcg
     2461 cgtgcgccct cctgttcgag caggcggcgc agatgatctg gggcccgcat cccctcttga
     2521 tcgaggcggg caccccgtgg atcggtccag cgggcctcgt catccggcgg tggggtgtga
     2581 tcgcgtttct ctcctccgcg gcgctgctgg gggggctgaa atgggttctc tcctcccgcc
     2641 ccgggctgcc gcttcgcctg atcgccgagg acgaggagat cgccggctcg ctcgggatgg
     2701 acgtcgatgc gatccggtac ctgacgttcg gcggggcctg cgcgatggcg gcggcggcgg
     2761 gcgccctcct ctccccttcg ggagcggtga cgccgacgat ggggcgcgca ccgctcctgc
     2821 tctccctcgt cgtcgtgatc gtcgccggga tggactccgt ggcggcgatc ttcctcctct
     2881 cgatcgggct gggaattttc ggcaacgcgt gcgcctggct cctctccccc cagtggtcgt
     2941 acgtcgccct gctcgccgcc gtctgcctgc tcctgtcggc gcgccccgcg ggaatcgtag
     3001 tactggccgg gcggcgggat tgagaaacgc ggcctccttc cgcgggggct ggctggcagc
     3061 cgggatcggg ctgctcctgc tcttcccgct ttcctccccc gacgccttct tcctcgacgt
     3121 gttcacgacc gggttcctgc tggccgcgtt cgcggcgtcg tgggacgtgg tcggcggcgt
     3181 ctcggggcag attacgctcg gtcacgctct ccccttcggg acggcggcgt acgcctgcgc
     3241 gctgctcacg agcttcgcgg ggtggccgct ccccctggcg gtggcggcgg cggtcctcct
     3301 ggccgccgcg gtgggcgcgg ggatcgggac gctctcggcg ccgctggccg gaccgttcgt
     3361 ggcgctgctc accctcgcgc tgggagagat cgcgcacgag gtggccgtcg ggcacacctt
     3421 cttctccggg ccgggggggt acgcgtgggg cggcgagggc gggatcccgg tggcgcttcc
     3481 gtggcgggac gcctctccgt tctcgtcgta ttacgcggcc ctcgccttcc tctgcatcgc
     3541 ctccttcggc atgctgcgct tctcgcgctc gggcgcgggg ctcatcctgc gcgcggtcgc
     3601 cgggtccccg atcacggccc aggcgtcggg ggtcccgatc gcccggttca agcggctggc
     3661 gttcgccgtc gggtccgccg tcgcgggagc ggcgggagcg ggcttcgtcc tccacgtcgg
     3721 gcgcgccacg gcgaccgatc tctccctcga gctctccttc caggccgcca cgttctccgc
     3781 cgtggggggg ctcgggacga tcgtgggtcc cgtcgtcgcg gcgctcgtcc tccatgtcct
     3841 cttccagggg ctctccctgc ccccttcggc gcgggtcctg ctctacgcgc tgaccctgct
     3901 ggtcaccctg cggttcttcc ccgggggggt cgtcgggacc gcgcgggacc tggcctcgcg
     3961 gctccgcggg agggaaggga cgcggccccc gcgccgccgc ggccggaacc gggccgggga
     4021 gggcacaccg tgaacacgct gctgtcggcg gaagggatca cgaaagcgtt cggcgcaacg
     4081 caagtcctct ccggcatcac cctatcggtc gccccggggg aaccggtagg gctgttcggc
     4141 ccgaacggat cggggaagac cacgctcgtc aacatcctct cggggcttct tctccccgat
     4201 gcgggcacgg tccggttcga ggggaaggag atcacccggc ttccgctcga cgcccggtac
     4261 cggctcggca tcgcgcggac cttccagatc ccgcacccgt accccgccct ctcggtccgc
     4321 gaggcggtgc gcgtcgccct cgcggccaac gccgggaaac ggaaggaaga gggcaggacg
     4381 cgggaagacg aggagggacc ggccgaggac ctcctcgtcc ggacgggact gttcaaccag
     4441 cggttcgtgc cgtgcgccca gctgtcccaa gggtgcctcc gccgcctcga gttcgcgcgc
     4501 gggctggcgt gccggccgaa gctgctgatc ctcgacgaga tcttctccgc cctctcggcc
     4561 gcggacgagg gggacctgat ggcgctgctg cgcacggcga accgcgacga gggaacggcg
     4621 ctcctgctgg tctcccataa cccgccgttg ctgaaggcgc tctgcaggcg catcctcgtg
     4681 ctcgaggagg ggcggatcgt gcgggagagg gcggtatgag tgtctcagtt cataaatacg
     4741 gtcagcaccg agagcgtcga tgcgccgcgt cggcaaggcg cgcgactgag gcgtactgtg
     4801 aagtacggcg caaggagcgc cccactcccg gacgaaatac gctccctggg gtaccctcga
     4861 ccgccgatac gatttccctt gatcggcatg tggggcacaa cgcagcacag ggcagcagcg
     4921 atggccatgg atggccaagt gccgcgaagg gcatggatgc ccgggagcgg ccggcgctcg
     4981 aatgcgaccg tatttatgaa ttgagacact agatgcccgg tgggacgtcc accctgaagg
     5041 tgcgcgacct caccgtcgcc ttcgggcagg tggtggcggt gcgggacgtc tccctcacgg
     5101 cacgggaggg ggagacggtg gcgatcttcg gcgcgaacgg ctcggggaag acgaccttcc
     5161 tcaaggcggt ggcggggctg atccagacca cgcggggaaa ggtcgcgtgg cggggcgagg
     5221 acgtgacggc gcttccggcc cacgagcggg ccgcgcgggg gatacggtac gtctccgacc
     5281 ggtcacgggt gggaacgagg atgacggtcc tcgagaacct cgaggccggc gcgtggctcc
     5341 ttccctccgc acggtggagc gcggcgcgcg agcgcgtgtt ctccctcttc cccgtgctgg
     5401 cggagaaggc gcgctctccc gcgggggtac tctcgggcgg agagcgccag atgctgatcc
     5461 tcgggcgggc gctggtggcg gagccgacgc ttctcctgat ggacgagccg ttcctcgggc
     5521 tctcccggga aatccgcgac cggctgctct ccgtaatcga aggtacgttg aaggggcggg
     5581 cgacgatcct tctcgccgag cacgacgcgg aaggggcgta ccggctcctc gaccggcacg
     5641 tcattttccg gaacggctcc gtggtccacg agggaacgcg gtcggacgtc gccgacgcga
     5701 aggaactggt ctctctgctg taccggcatt tccgtccggg ggaagccccg ggggagacga
     5761 tcggcgaggc agaacgaaag gaaaaaccat gagagggaag acgggcgggg aaaaggaaaa
     5821 cggggagagg gaaccggtat cggtgacggt gacgctgccg tcggcgctgg tcgatcagtt
     5881 gaaggagttc tgccgcaact gccgcgtgac gcgggacatc gtggtggagc gcgccctggt
     5941 cgaatatttc cgcgaaggag acatgagcca ctaagcgcat caattcatca ctacgtcgac
     6001 gttccgacgc aggagactcc gcacgccgga ggcgcatcga agggggtatc acgcgaagat
     6061 caaagcgcct ccggttacgt tgccgtattc atgaaacgca acggagagtc accggtcgag
     6121 ggattcggag atgagccccg cggtcttttc actgcgctcg gcatcgcggt cggccacgat
     6181 gcgggccatc cgctcccgcc acggatccat ccggaacccg gcccggtcgc gcacgcaggc
     6241 gtcgatcaga tcgcggtaga accggaccat ccggcccacc agcggcaccg agtactcccg
     6301 tccctggatc ttgatcgtgt cgaccccgag gtcgatcaga tcgggaatgt cgtcggcgag
     6361 gaagtaggcc cggttcggca gctccacgcc ggactcctcg agctccccgg ccgcgcccac
     6421 ccggtcccag tcggagagac agatccggta gcagaggcct ccgcggttcg ggctgcccgg
     6481 gaaatggtct ttccccgatg catccacccg gtgcccgtac ttcgtgtagc tgctcatcgt
     6541 gcagcgcccg aggagcgtgt agcaggtggt ggcgtgaacg aggacctcca gcccgacccc
     6601 cgcttccacc ttgattctcc gcatcgtcgc cttccccatg cggcactcgg cgaccacctg
     6661 ggaggcgccc gcttccttgt agaagagggc gtcctgaacg ttgatgatgg tgcatcccac
     6721 gctcgcgtgg aggatcgtgt cgggatggcg gtttttcagc gccatcatca gcccggggtc
     6781 ggtgaggatg aagtcgcgga cgccggcgtc gtggagcgcg tcggtcctga cgaggaagag
     6841 ggggacctcg gaggatgccg ggagggtgtt gaacgccacc cggaggatcc tgccggcgga
     6901 acgggcgtac cgcgccgcct cgacgatcgc ggcgtcgtcc atctcgtact gcgcccggcg
     6961 gcgactccac cccgtggcgc ccacatagac ggcgtccgcc ccgttgtcga aggcggcacg
     7021 caccatgtcc atggaaccgc cggatgccaa cagctcggcc atcgacgtca cccctccgtg
     7081 cgcggatccc gttcgttggg aagacgcaac tacgaattat acctcagggt tagcgtcctg
     7141 taaaaccgtt ccgacgtacg ttcggccggc catcccgaag cagtacccgt tgcacaggcc
     7201 ccgcggggca tggcgcctcg cggcgtcggc ccaccgttcc tcgagcgcga acgaaccgcc
     7261 ggcgaaggcc cgggccagcg cctcccggta ggcggcgccg atctccgacc ggtacgcggc
     7321 ggtttcgtag agcccctcga cccggaagac gcggaagccg tccgcgacca gccgcgggag
     7381 gtgctccagc aggcacatgt cccggcccga gaggaccccc ttcccgaccg tcttcaagac
     7441 ccactggcgg gaggtgagcc agtggacctc cccgcaggcg gacgggcact tcgggtcggt
     7501 ctcctcgggc tccgtgagca ggaagcagcg gtcggtgacc ccgaggggga tcttcccgtg
     7561 gacgagcacc tcgacctcga cgcccgcctc gcgggcgagg atcgccatct cctcgaggga
     7621 cacctcggcg ttgggacgta cccggaccgc ccccgcgtcc cgcatgaccc cggccgcgag
     7681 gtgggtatag acgttcgcgt aggcgcccat gtgggccgga acttgccgcc ccttctcccg
     7741 cacgatccgg aggaccccca tgttgtggat ctcgagggcg tccgccccgg cggccgcggc
     7801 ggcgtcgacc aggcggccga catgggggag gtccgcctcg cgcggcacgg ccggcgtgga
     7861 gacgtacgcg cgcttccccg ccgcgtgaag gcgggagacg acaccgggga gaaggtcgag
     7921 cgcttcggcg tagttccctt cgacgcgccg gcaatacggg cttcccaggc acacggcgtc
     7981 gtagggagag aggtcggtcc cctcgagctc ccctggggag gcgatactga cgatgagttc
     8041 gaatcctatt gccgtccgcc ccgtttcagg tcgatcagga ccagcttgat gaccgtcttg
     8101 agggtctcga agaccccgac ccccttggag gccgccgcct cgaactcggg gacgttgcgc
     8161 cggttgagga gcgcgtggag ctcggccagc gaggcgacgt tcggcaggtc gcgcttgttg
     8221 tactggatca ccagggggag cctgtcgggg tcgtacccct gctcccggag gttcatccgg
     8281 aggttctcca tcgactcctc gtttgagtcc gcgcgagtga gctgcgagtc ggcgcagaag
     8341 acgacgccgt cgacgccgcg caggatgagc cgacggctgg cgtcgtagaa cacctggccg
     8401 ggaacggtgt acaggtggaa gcgcgtcttg aagccgcgga tctcgcccag cgacaggggg
     8461 aggaagtcga aaaagagggt ccgctccgtc tcggtggcga gggagatcat cttcccgcgc
     8521 gtctcggggt tcatccgctt gtggatgact tgcagattgg tcgttttccc gcacaggccg
     8581 ggcccgtaat agacgatctt gcagttgatc tcgcgggagg agtagttgat gaaggccatt
     8641 agatgaaggc tattagatga aggacattac ttgaagaggc tttcgatgtc cgcttcggtc
     8701 aactcctcga ctcccccggg ctcatcttcg ttggcggcca tcgccaccgc catgatcgcg
     8761 gtgaaacgcg ccgaggcctg acggacgcgc aggcggacaa ggccgaccga ggagcgccgg
     8821 tcgaagacga ctacgaggat gaggcgctcc gccacgacgg agaggtgcat gttgtcccgc
     8881 tccccctcgt ggaagaggat ggagaactcc ttctccccga tgaggttggc gagacccccg
     8941 gtggcggcga tgtttcccgc ggcgagggag gccagggacg tcgtgtcgaa cccgacggca
     9001 tccccggcgg aagcgaggag ggtcccgtcc ttgtcgacca ggaagacgac cttcgcggag
     9061 gcgtccacca gcaa
//
LOCUS       DASVJL010000358         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_154040, whole genome shotgun sequence.
ACCESSION   DASVJL010000358 DASVJL010000000
VERSION     DASVJL010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154040"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VF357_07590"
     CDS             <1..408
                     /locus_tag="VF357_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7524049.1"
                     /translation="DYFVAGMGTGGTLMGTGAYLRSRKRDIRIVGVEPKPGHKIQGLK
                     SMKEAIVPAIYREENLDEKLIVDDDPAFETTRRLAVEEGLFVGMSSGAAVAGAIQLAR
                     RLSSGTIVALLPDRGDRYLSTSLFRSTCGACPP"
     gene            complement(476..1291)
                     /locus_tag="VF357_07595"
     CDS             complement(476..1291)
                     /locus_tag="VF357_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX7524050.1"
                     /translation="MGILQGRKGLILGVANEKSIAWGVARACHREGAELGFNYLGEAL
                     MKRVHPLAESIHSSFIEPLDVSDDAQLDDFFAKVEARWGRLDFIVHSIAFANRESLKG
                     NFRDTSRADFHLALDISAYSFIACARRAARLMGPGGSMVTMSYLGAVRAVPNYNVMGV
                     AKAALEAATRYLAHDLGPEGIRVNAVSAGPIRTLAASGVVDFRKLMEKNARGASLRRN
                     VTQDEVGNAAAYLLSDWSSGVTGEVHYVDAGFNIGGGDPGVEPAASGSSSPSS"
     gene            complement(1366..1455)
                     /locus_tag="VF357_07600"
     tRNA            complement(1366..1455)
                     /locus_tag="VF357_07600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1418..1420),aa:Ser,seq:cga)
     gene            complement(1513..2235)
                     /locus_tag="VF357_07605"
     CDS             complement(1513..2235)
                     /locus_tag="VF357_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7524051.1"
                     /translation="MDCRDVRPHLLDSQRGRLNPETEEKVRAHIAGCLSCSHAEEAER
                     ALSDLLERRLPQYPAPIALKLDLATRWPSPPVARLAWWVRWGRFLVPARESMVAEAVN
                     DHLRILESQHPLEIESGNFHQVKPWFEGRLDFAPVVPFLGDEEFPLRGGAIGYYLDRK
                     AAVFEYGSRLPAVSLFVFRAEGLPWPARGLESLGKVRAYSVASRGYNTILWREGEFGY
                     AIVSDVDSSVLRKLAVKLSGGG"
     gene            complement(2244..2435)
                     /locus_tag="VF357_07610"
     CDS             complement(2244..2435)
                     /locus_tag="VF357_07610"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX7524052.1"
                     /translation="MARDIERALMCLSEESRTAVLLDLEGFTEEEVAQVTGCAVGTVK
                     SRLARARSALRRYLKDYGG"
BASE COUNT          497 a          834 c          796 g          423 t
ORIGIN      
        1 gactacttcg tggcggggat gggaacgggg ggaacgctca tgggtacggg tgcgtacctg
       61 cgcagcagaa agagggatat ccggatcgtc ggggtagaac ccaaaccagg ccataagatc
      121 cagggactga aaagcatgaa ggaggccatc gtcccggcga tctaccggga ggagaacctc
      181 gacgagaaat tgatcgtcga cgacgacccc gcgtttgaaa ccactcggcg ccttgcggtt
      241 gaggaagggc ttttcgtcgg gatgtcgagc ggcgcggccg tggcgggcgc catccagctg
      301 gcgaggcgtc tttcgtccgg aacaatcgta gcgttgttgc cggaccgggg ggaccgatac
      361 ctgagcacct ccctgttccg gtccacgtgc ggagcctgcc ctccctgacg gagggatgaa
      421 ggacaccgtg ctacggggga aagggcacgc tcacatctcc ctctcccaat aacgctcagg
      481 acgaggggga tgaggagccc gatgcggccg gttcgacgcc cgggtcgccg ccgccgatgt
      541 tgaaccccgc gtcgacgtag tgcacttcgc cggtcacgcc ggaagaccag tccgaaagaa
      601 ggtacgcggc ggcgttcccc acctcgtcct gcgtcacgtt ccgtcggagc gaggcgccac
      661 gggcgttctt ctccatcagt ttgcggaaat cgaccacgcc ggaggcggcc agcgtacgga
      721 tcggcccggc cgagacggcg ttgacgcgaa tcccttccgg gccgaggtcg tgggccaggt
      781 aacgggtcgc cgcttcgagg gcggccttcg ccacacccat cacgttgtag ttcggcacgg
      841 cgcgcaccgc cccgaggtaa ctcatcgtca ccatggaccc ccccggtccc atcaaccggg
      901 ccgcccgccg ggcgcaggca atgaaggagt aggcggaaat atccagcgcg agatggaaat
      961 cggcgcgcga ggtatcccgg aaattgcctt tcagcgattc cctgttggca aaggcgatgg
     1021 agtgcacgat gaagtcgagc cgcccccaac gggcctcgac cttggcgaag aaatcgtcga
     1081 gctgggcgtc gtcgctgacg tcgagcggtt cgatgaagct cgaatggatc gactccgcca
     1141 agggatgaac gcgtttcatc agcgcctcgc cgagatagtt gaatccgagc tcggccccct
     1201 cgcggtgaca ggctctggcc acgccccatg cgatggattt ctcgttggcg acgccaagga
     1261 tcagcccttt cctgccttgc agaatgccca cgagacacct cccggtttcg atcacactca
     1321 atcataacag ggagcaccgg atagagcacg tggatcggag gatcgcggag ggggtgggat
     1381 tcgaccccac gtggcggttc gcaccgccaa gtcgatttcg agtcgacccc gttacggcaa
     1441 cttcggtacc cctccgcggg ggaaggcagg cagcacagcg ggcggagggg gaccaggccc
     1501 aagaaccgtg gttcagcctc ctcccgaaag cttcaccgca agtttgcgga gaacggagga
     1561 atccacatcg gaaacgatgg cgtagccgaa ctcaccctcc cgccagagga tggtgttata
     1621 tcctcggctc gctacggaat acgcccgaac cttccccaag gactccaagc cgcgggccgg
     1681 ccacggaagc ccctccgcac gaaagacgaa aagtgatacg gcagggagtc ggctgccata
     1741 ttcgaaaacc gccgccttgc gatcgagata atagccgatg gcacctcccc ggagaggaaa
     1801 ctcctcatcc cccaggaacg gaaccaccgg cgcgaagtcg agccggccct cgaaccacgg
     1861 ctttacttgg tggaagttcc cgctttctat ctccaagggg tgttggctct ccaggatgcg
     1921 gaggtgatcg ttgaccgctt ccgcaaccat ggattcgcgc gccggaacga ggaaacgccc
     1981 ccatcgtacc caccacgcga gtcttgcaac aggcggcgac ggccaccgcg tcgcgaggtc
     2041 caacttcagg gcaatggggg cggggtactg cgggagcctg cgctcgagaa gatcagagag
     2101 cgcccgttcc gcctcttcgg catgggaaca cgacaggcat ccggcaatgt gcgcgcggac
     2161 cttttcttcc gtttccgggt tcagccgccc tcgctgggaa tcaaggagat gcggtcgcac
     2221 gtccctgcaa tccatatctc gccctacccc ccgtagtcct tcagatatct ccggagcgcg
     2281 gagcgggcgc gcgccagtcg ggacttcacg gttcccaccg cgcaccctgt cacctgcgcc
     2341 acctcttcct ccgtgaatcc ttccaggtcc aggaggacgg cggtgcgcga ttcctcggac
     2401 agacacatca aagccctctc aatgtcacgg gccacgatct tgcgaaactg ctccagttcc
     2461 ctgtcgcttc ccggccaggc tcccgaaccg gtctcctctc cgtacggtcc acggtgtcca
     2521 ggccgcccac cgtcggatca tgctgccgcc
//
LOCUS       DASVJL010000359         1815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_905301, whole genome shotgun sequence.
ACCESSION   DASVJL010000359 DASVJL010000000
VERSION     DASVJL010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905301"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VF357_07615"
     CDS             complement(<1..538)
                     /locus_tag="VF357_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX7524053.1"
                     /translation="MTGVALLDQHLLSVLIALPLIGAGLLLFFPREGDAAIRTFTLLV
                     TVVEFILSLLVVARFDVAIAGMQLVERTPWIPQYGISYIVGVDGISLWILMLTTFIMP
                     ITILSTWSAVTKNVKEFMVFMLILETAMVGVFLATDLFLFYIFWELVLIPMYFLIGVW
                     GGERRIYAAIKFFLYTFVG"
     gene            complement(535..>1815)
                     /locus_tag="VF357_07620"
     CDS             complement(535..>1815)
                     /locus_tag="VF357_07620"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX7524054.1"
                     /translation="KSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYMVARSSAL
                     FLLAPDTMMVVAVIGAVTAIFSATIGICQTDIKRVLAYSTVSQLGYMFLACGVGAFTA
                     GIFHLMTHAFFKALLFLGSGSVIHALSGEQDMRKMGGLKKYVPVTFATMFAATLAITG
                     IPGLSGFFSKDEILWQAFSSPHGSPVLWGIGALAAGITAFYMFRLVFLTFFGKSRMDP
                     GVEKHAHESPWTMTVPLAILAVLSVAGGWIGIPAILGGGNLFEHWLAPVFHPVAAAGA
                     HGTAAAGAHGAEGAASHPAALEIGLMALSVAIALCGIGLAYFLYRVRTGKPAEIAKRW
                     STLYDVVYHKYYIDEFYEWAVIRRIVTFSTGLWQMFDALFIDGIVNGAADLVRAAGDR
                     VRRLQGGVVGGYAFSLLAGAVLLVGYILYRSVVQ"
BASE COUNT          371 a          586 c          614 g          244 t
ORIGIN      
        1 agccgacaaa ggtgtacagg aagaacttga tcgccgcgta gatccgccgc tcgccccccc
       61 acacgccgat caggaagtac atcgggatga ggaccagctc ccagaagatg tagaagagga
      121 acaggtcggt ggcgaggaac acgccgacca tcgcggtctc gaggatgagc atgaagacca
      181 tgaactcctt cacgttcttc gtcaccgccg accaggtcga gaggatcgtg atcggcatga
      241 tgaaggtcgt gagcatcagg atccacaggg agatcccatc caccccgacg atgtaggaga
      301 taccgtattg cgggatccag ggggtccgct ccaccagctg catcccggcg atcgcgacgt
      361 cgaagcgcgc caccaccagc agggagagga tgaattccac gaccgtcacg aggagcgtga
      421 acgtccggat cgcggcgtcc ccttccctcg ggaagaacag gagcagcccc gcgccgatca
      481 gcggcagcgc gatcagcacc gacaggaggt gctggtcgag cagcgcaacg ccggtcattg
      541 gaccacgctc cggtagagga tgtacccgac gaggaggacc gcccctgcca gcagggagaa
      601 ggcgtacccg ccgacgaccc ctccctggag cctgcgcacc cggtcgcccg cggcgcgaac
      661 gaggtctgcg gcgccgttga cgatcccgtc gatgaagagg gcgtcgaaca tctgccacaa
      721 cccggtggag aaggtaacga ttcgccggat caccgcccac tcgtagaact cgtcgatgta
      781 gtacttgtga tacacgacgt cgtagagggt ggaccacctt ttcgcgatct ccgccggctt
      841 cccggtgcgg acccggtaca ggaagtacgc gaggccgatg ccgcacaggg cgatcgcgac
      901 cgacagcgcc atcaggccga tctcgagggc cgccgggtgg gacgcggcac cttccgcacc
      961 atgggcgccc gccgcggcgg tcccgtgcgc gcccgcggcg gcgacggggt gaaagaccgg
     1021 cgccaaccaa tgctcgaaga ggttgccgcc gcccaggatg gccggtatcc cgatccaccc
     1081 gccggcgacc gacaggaccg cgaggatcgc cagcggcacg gtcatcgtcc agggggattc
     1141 gtgggcgtgc ttctcgaccc cggggtccat ccgggatttt ccgaagaagg tgaggaagac
     1201 gagccggaac atgtagaacg ccgtgatccc cgcggcgagc gctccgatgc cccacaggac
     1261 cggactcccg tggggcgacg agaacgcctg ccacaggatc tcgtccttgg agaagaagcc
     1321 cgacaacccc gggatcccgg tgatcgccag cgtggcggcg aacatcgtgg cgaaggtgac
     1381 gggaacgtac ttcttcagcc cccccatctt ccgcatgtcc tgctccccgg agagggcgtg
     1441 gatgaccgag ccggagccga ggaagaggag cgccttgaag aaggcgtgcg tcatcaggtg
     1501 gaagatcccc gccgtgaagg cgccgacgcc gcacgcgagg aacatgtacc ccagctgcga
     1561 cacggtggag tacgccagga cccgcttgat gtcggtctgg cagatgccga tcgtggccga
     1621 gaagatcgcg gtgaccgcgc cgatgaccgc cacgaccatc atcgtgtcgg gggccaggag
     1681 gaaaagggcg ctggaacggg cgaccatgta gacgcccgcg gtgaccatcg tggccgcgtg
     1741 gatgagcgcc gacaccggcg tgggaccctc catcgcgtcg gggagccaga cgtacagcgg
     1801 gatctgcgcg gactt
//
LOCUS       DASVJL010000360         5176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_810187, whole genome shotgun sequence.
ACCESSION   DASVJL010000360 DASVJL010000000
VERSION     DASVJL010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810187"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VF357_07625"
     CDS             <1..214
                     /locus_tag="VF357_07625"
                     /inference="COORDINATES: protein motif:HMM:NF021941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524055.1"
                     /translation="DFGQEAKRLAKEVEKAKAEYARVAGQLGNARFTAKAPPDIVDAL
                     RDREKALEQKIAKLGKNFELVSRYLT"
     gene            211..1035
                     /gene="nadC"
                     /locus_tag="VF357_07630"
     CDS             211..1035
                     /gene="nadC"
                     /locus_tag="VF357_07630"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX7524056.1"
                     /translation="MISRYLYEETARAALREDAPFGDPFGEAFHGDASGLFLAGGDGV
                     LCGGAVASEVFRQVDPSVFISFSPDGCRVARGDRVGKASGPAGSLLRAERGALNFLQR
                     LSGVATATSLYASRIAPHGTKLLDTRKTTPLLRVLEKYAVRVGGGTNHRFSLSDGILI
                     KENGIRAAGGIAPAVAAARSTAHHLLRVEVEAETLSDVEAAVAAGADAVLLDNMPPSM
                     VREAVARFGGIVFLEASGGIHLGNVEEYARTGVAAVAVGAITHSAPALDISFELST"
     gene            1188..1877
                     /locus_tag="VF357_07635"
     CDS             1188..1877
                     /locus_tag="VF357_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524057.1"
                     /translation="MRALLTRRIDALDASGYLRDATLGVSILLVLLFLSPAAGRAAEG
                     YRVYESTAASVNSEVLFVSDVAREVCFLRCAAMPGTVGEILSLRQARDRLVLDTLALQ
                     EQRKLLLGAVDNATLEGAAREAETRMAACSSPCKREIAFGETREWIRRKLLLRDFFNR
                     RVAVFVEVKDDDVEKELARRSSAPGSGAIPSLEEVRKEMLDARIAQEIRNWQTRAASK
                     SRMILSPMEDR"
     gene            1874..2257
                     /gene="queD"
                     /locus_tag="VF357_07640"
     CDS             1874..2257
                     /gene="queD"
                     /locus_tag="VF357_07640"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEX7524058.1"
                     /translation="MSSGLYALTVRTSFAAAHRLREYEGNCERLHGHNWQVEVTVESP
                     TLDERGIALDFRILKTSLHDLLSRFDHRYLNEVPPFDEMNPSSENLARHLYEEMGKSV
                     PAPAHVSRVTVWESDDARADYSRRD"
     gene            complement(2267..2467)
                     /locus_tag="VF357_07645"
     CDS             complement(2267..2467)
                     /locus_tag="VF357_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEX7524059.1"
                     /translation="MIDKEMKIEDVLRRYPQAIPVFERFGIDCAQCQLSEYENLEHGA
                     KVHGIDLSALLRELNESLAVKG"
     gene            complement(2492..3352)
                     /locus_tag="VF357_07650"
     CDS             complement(2492..3352)
                     /locus_tag="VF357_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEX7524060.1"
                     /translation="MTGHPSPLRVGRIPYANLIPIFHGLRTGGVPGGVSFVDGHPSEL
                     NRKLRDGELDLSPSSSIEYAVRPDRYLLCPDISISSRRRVMSVLLLSNGPLRQLPPEP
                     IAVTGSSDTSILLLEILLRESLGRANLLVRTELPAREALRRYPAHLVIGDEAIRAAVD
                     GVAPHVTDLGEWWRRETGKPFVFALWIAAREAWEDRRDSLSRFSAALLEAKRTAQASI
                     RRGEYPWGGPDWIPSAFRDAYWRCLSYDLKEETEGLSLFYELAAKIGRIPAAPPLRFL
                     EIDKRIPVVK"
     gene            complement(3349..4554)
                     /locus_tag="VF357_07655"
     CDS             complement(3349..4554)
                     /locus_tag="VF357_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7524061.1"
                     /translation="MRRIDFVEVAFDSLQINRLRSALTMLGVIIGVAAVILLVSLGQG
                     TKNYVEEQFAGLGSNILIVTPGKIETKGGPPIIGSVRHKLTLNDSRILEKKGYLFRGV
                     APVVFGTAEVRYQNRSRNVSVLGVTPAFQDVRNLHVEIGSFLGEADVDARRRVCVLGR
                     TVKRELFGNANALGQTVKVAGTLFRVMGIMEKKGISLGIDIDDLVFVPVRTAQELFDI
                     DGLIEILISVRNTNDLDSGKELAASLLKRAHNRNEDFTITNQAAILSSLYTILDTLTY
                     VLGGIAAISLLVGGIGIMNIMLVTVKERTNEIGIRKAVGARNRDILLQFLFESTVLAT
                     VGGIAGIVAGVAGAGALKLFVPKLPVSIPAWSILLSFTFSAFVGVFFGVYPAKKAAAL
                     NPIEALRYE"
     gene            complement(4551..>5176)
                     /locus_tag="VF357_07660"
     CDS             complement(4551..>5176)
                     /locus_tag="VF357_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7524062.1"
                     /translation="DVDLEIGAGEFVAVMGPSGSGKSTLMNILGCLDVPTSGTYEIKG
                     TSVAGLSPDHLSDLRNREIGFVFQVFHLLPRYTALRNVELPLIYAGVPREERARRALE
                     ELRAMGLEDRKGHLTNELSGGQKQRVAIARALVNKPALLLADEPTGNLDTRSGEELMD
                     ILARLNASGVTVVLVTHDPAIARWAHRTVYIVDGRIVSVERFREIRG"
BASE COUNT          859 a         1665 c         1751 g          901 t
ORIGIN      
        1 cgacttcggg caggaagcga agcgtctggc caaggaggtg gagaaggcga aggcggagta
       61 cgcccgcgtc gccgggcaac tggggaacgc gcggttcacc gccaaggccc cccccgacat
      121 cgtcgacgcc cttcgggacc gggagaaagc gctggaacag aagatcgcca agctcgggaa
      181 gaatttcgag ctggtgagcc gatacctgac gtgatctccc ggtatctgta cgaggagacc
      241 gcccgcgcgg ccctgcgcga ggacgccccg ttcggcgacc ccttcggaga ggcattccac
      301 ggggacgcgt cgggcctgtt cctggcggga ggtgatgggg tgttgtgcgg cggggcggtg
      361 gcgtcggagg tgttccgcca ggtcgaccct tccgttttca tttccttctc ccccgatggg
      421 tgtcgcgtgg cccgcggcga ccgggtcggg aaagcgtccg gtcctgccgg ctctctcttg
      481 agagcggagc ggggggccct caacttcctc cagcggctct cgggcgtggc caccgcgaca
      541 tccctttacg cttcgcggat cgccccgcac ggcacgaagt tgctcgacac gcggaaaaca
      601 accccgctac tccgggttct cgagaaatac gccgtccggg tggggggcgg gacgaaccac
      661 cggttctccc tttcggacgg gatcctgatc aaggagaacg ggatccgcgc ggccgggggg
      721 atcgccccgg cggtcgccgc cgcgcgttcg acggcgcacc acctcctccg cgtcgaggtc
      781 gaggcggaga cgctttcgga cgtggaggcg gcggttgcgg cgggggccga cgcggtgctc
      841 ctcgacaaca tgccgccgtc gatggtccgg gaggcggtcg cgcgcttcgg cgggatcgtc
      901 ttccttgagg cgtccggggg gatccacctc gggaacgtcg aggagtacgc ccgcacgggg
      961 gtggccgccg tcgccgtcgg tgcgatcacc cactccgccc cggccctcga catctctttc
     1021 gagctgtcga cgtgaaacta gtgcagtgtc tcgcaaatac cctggcattc gagcgtcggc
     1081 cgctcccggg catccatgcc ctccgcggca ctaggccatc catggccgtc gctactgcct
     1141 ccagcttcgt tgcgcaagtt cgccgtactc tcccggtacg ccttcacttg cgcgccttgc
     1201 tgacgcggcg catcgacgct ctcgatgcgt caggctattt gcgagacgct acactaggcg
     1261 tttccattct cctggtcctc ctgttccttt ctcccgcggc ggggcgcgcg gccgaggggt
     1321 accgtgtcta cgagtccacc gccgcgtcgg tgaacagcga ggtgcttttc gtctccgacg
     1381 tcgcgcggga ggtgtgcttc ctccggtgcg cggcgatgcc cgggaccgtg ggggagatcc
     1441 tttccctccg tcaggcgcgg gatcgcctgg tcctcgacac gctggcgctc caggagcagc
     1501 ggaagcttct cctcggggcg gtggacaacg cgacgctgga gggggccgcg cgggaggcgg
     1561 agacccggat ggccgcgtgc tcgtccccct gcaaacggga gatcgccttc ggggaaaccc
     1621 gggaatggat ccgccggaaa ctgctcctcc gggatttctt caaccggcgg gtcgccgtgt
     1681 tcgtggaggt gaaggacgac gacgtggaga aggagcttgc gcgcaggtct tccgcccccg
     1741 gaagcggcgc gataccgtcc ttggaggagg tccggaaaga gatgctcgac gcgcggatcg
     1801 cgcaggagat ccgaaactgg caaacccgcg ccgcatcgaa atccaggatg atcctttcgc
     1861 ccatggagga tcgatgagtt caggcctgta tgccctcacc gtgcgaacgt ccttcgccgc
     1921 cgcccatcgt ctgcgcgagt atgaagggaa ctgcgagcgg ctccacgggc acaactggca
     1981 ggtggaagtt acggtggagt cgccgacgct cgacgaacgt gggatcgccc tcgacttccg
     2041 gatcctcaag acgtcgcttc acgacctgct ctcccggttc gaccaccggt atctgaacga
     2101 agttccgccg ttcgacgaaa tgaatccgtc gtcggagaat ctcgcgcgcc acttgtacga
     2161 ggaaatgggc aaatcggtcc cggcgccggc gcacgtgtcc cgtgtcaccg tgtgggaatc
     2221 cgacgatgcc cgggccgact actcccggcg cgactgaccg gatcctctac cccttcactg
     2281 ccagcgactc gttcagctcc ctcagcagcg ccgaaaggtc gatcccgtgg accttggcgc
     2341 cgtgctcaag gttttcgtat tcggagagct ggcactgggc gcaatcgatc ccgaaccgct
     2401 cgaagaccgg aatcgcctgc ggatatcgac ggagaacgtc ctcgatcttc atctccttat
     2461 cgatcatcgt gtctcctccc ggcgggatgc cttattttac cacggggatc ctcttgtcga
     2521 tttcaaggaa acggagaggt ggcgccgcgg ggatccggcc gattttcgcc gccagctcgt
     2581 agaacaggga gagaccctcc gtctcctcct tgaggtcgta ggagaggcag cgccaatagg
     2641 cgtcccggaa ggcggatggg atccagtcag ggcctcccca cgggtattcc ccccggcgga
     2701 tcgacgcctg cgcggtccgt ttcgcctcca gaagggcggc ggagaaccgg gacagggaat
     2761 ccctgcgatc ctcccacgcc tcccgggcgg cgatccaaag ggcgaaaacg aacggtttcc
     2821 ctgtctcccg ccgccaccac tcaccgaggt cggtgacgtg cggcgccacg ccgtcaacgg
     2881 ccgcccggat cgcctcgtcc ccgatcacga gatgggccgg gtagcggcgc agcgcctctc
     2941 tcgcgggaag ctccgttcgc acgagcaggt tcgcgcgccc cagcgactcg cggaggagga
     3001 tctcgagcag caggatcgat gtatccgaac tcccggtgac cgcgatcggc tcggggggaa
     3061 gctgccggag cggtccgttc gacaggagca ggacgctcat cacgcggcgc ctcgaggaga
     3121 tggaaatgtc ggggcacagg agatatcggt ccgggcggac ggcgtactcg atggaggagg
     3181 agggggacag gtcgagctcc ccgtcccgga gcttccggtt cagttcggag gggtgcccgt
     3241 cgacgaacga aactcccccc ggcacacccc cggtcctgag accgtggaag atggggatga
     3301 gattggcgta cgggatgcgt ccgacgcgca gcggtgaagg atgcccggtc attcgtatct
     3361 cagcgcctcg atggggttga gtgccgccgc cttcttcgcg gggtacacac cgaagaatac
     3421 accgacgaag gcggagaagg tgaaggagag gaggatggac catgccggga tggaaacggg
     3481 cagtttcggg acgaagagct tgagggcccc ggcgccggcg actccggcga cgatgccggc
     3541 gatcccgccg acggtggcca ggacggtgga ctcgaagagg aactgcagga ggatgtcccg
     3601 gttccgcgcc cccaccgcct tgcggatgcc gatctcgttg gtgcgttcct tcaccgtcac
     3661 cagcatgatg ttcatgatcc cgattcctcc gaccaggagc gagatcgcgg cgatcccgcc
     3721 gagcacgtag gtgagcgtgt cgaggatggt gtagagggag gacaggatgg ccgcctggtt
     3781 cgtgatggtg aagtcctcgt tccggttgtg ggcgcgcttg aggagggacg ccgccagctc
     3841 ctttccgcta tcgaggtcgt tcgtgttccg gacggagatg aggatctcga tcagcccgtc
     3901 gatgtcgaac agttcctgcg cggtccggac ggggacgaag accaggtcgt cgatgtcgat
     3961 tcccaggctg atccccttct tctccatgat tcccatcacg cggaagagag tgccggcgac
     4021 cttcaccgtc tggcccaagg cgtttgcgtt cccgaagagt tcgcgcttca cggtccgccc
     4081 gaggacgcag acgcgccgcc gggcgtccac gtcagcctcg ccgagaaacg agccgatctc
     4141 gacgtgaaga ttccgtacgt cctggaaggc gggagttacg ccgagtacgg agacgttgcg
     4201 cgaccggttc tggtaccgca cctccgccgt gccgaagact accggcgcga cccctctgaa
     4261 caggtacccc ttcttttcca ggatccggga gtcgttcagg gtgagcttgt ggcgcaccga
     4321 gccgatgatc gggggcccac ccttcgtctc gatctttccc ggggtgacga tcaggatgtt
     4381 gctcccgagc ccggcgaact gttcctcgac gtagttcttc gttccctggc ccagcgatac
     4441 gagaaggatg accgccgcga caccgatgat cacaccgagc atcgtgaggg cggatcgcaa
     4501 ccggttgatc tggagggaat cgaacgccac ctcgacgaag tcgatccgcc tcatccccgg
     4561 atctcccgga accgctcgac ggatacgatc ctcccgtcga cgatgtagac cgtccggtgc
     4621 gcccagcgcg cgatcgccgg gtcgtgggtg acgaggacga ccgtgacgcc cgatgcgttc
     4681 agcctcgcga ggatgtccat cagctcttct ccggaccggg tgtcgaggtt ccccgtgggc
     4741 tcgtccgcga gcaggagggc gggtttgttg accagagccc gcgcaatggc gacacgctgc
     4801 ttctgcccgc cggagagttc gttggtgagg tgccccttcc gatcttcgag gcccatcgcg
     4861 cgcaattcct ccagggcgcg ccgggcgcgc tcttcgcggg gcacgccggc gtagatgagc
     4921 ggcagttcca cgtttcgaag cgccgtgtag cgggggagca ggtggaacac ctggaagacg
     4981 aagccgatct cgcggttgcg cagatccgac agatggtcgg gtgagagtcc cgcgacggac
     5041 gttcccttga tttcataggt ccccgacgtc gggacgtcga ggcagcccag gatgttcatg
     5101 agggtagatt ttcccgaacc ggaggggccc atcacggcga cgaactcccc ggcgccgatc
     5161 tcgaggtcca cgtcgg
//
LOCUS       DASVJL010000361         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_529452, whole genome shotgun sequence.
ACCESSION   DASVJL010000361 DASVJL010000000
VERSION     DASVJL010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529452"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /gene="nrfD"
                     /locus_tag="VF357_07665"
     CDS             <1..875
                     /gene="nrfD"
                     /locus_tag="VF357_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX7524063.1"
                     /translation="PDLAIARDHATGWRKKFYAVLALGFRGTHNEWRRYHIASTIFAI
                     LIIPVAVSVHSIVGWDFGMAKVPGWHSTIFAPYFVTGAIFSGIAAVVIVMATLRKVFH
                     LERVLTVVQFENLGKLLCTMSLLWGYFYFSDFLTVWYNRNPEEWEVFRSYAGDYLPLF
                     LVMIGCNFVISFPLLCMRRVRRSIPALSFVSLLVVIGMYAERFLIVVPSLARRNDPFI
                     WHNYIPSWVEFSIMAGALSMFLLLYTIFTKLFPIMAISDIQEHLFHTTDRKIGEVTAD
                     SITMPHDTGKGGHG"
     gene            872..1396
                     /locus_tag="VF357_07670"
     CDS             872..1396
                     /locus_tag="VF357_07670"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HEX7524064.1"
                     /translation="MNGIVVVGLFDAVDPAAAAARALRALPLPEGDVTTLSPMALPDG
                     AVCKDPRGIGFPGFTLVGWFVGALAGLALALGTYLLYPLITGGKAIISVPPTLIVTYE
                     VAMLGALAGTVIAGFREMGLCRFRTRLIHDPRIQEGMILLCARVSGEEQTRRAMEAMK
                     SAGGIDVRPEEGEL"
     gene            1393..>1839
                     /locus_tag="VF357_07675"
     CDS             1393..>1839
                     /locus_tag="VF357_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7524065.1"
                     /translation="MRRISLARLIVFAAAFLAMALLAGCEKLDRNMYDNPAFRPQEEP
                     VRLPPAGSVPTKGLEHTPKPGTQEAAALKNPEKVTEFSLLTGKELFGIYCTPCHGASG
                     KGDGPVAKKFVPTPADISGTGYGGKYPEGELFAVVTHGKNGMPPFRS"
BASE COUNT          299 a          613 c          578 g          349 t
ORIGIN      
        1 tccccgacct ggcgatcgcc cgggaccatg cgacagggtg gcggaagaaa ttctatgccg
       61 ttctcgcgct gggattccgg ggaacgcaca acgagtggcg gcggtaccac atcgcctcga
      121 cgatcttcgc gatcctcatc atcccggttg ccgtatcggt ccactcgatc gtcggctggg
      181 acttcgggat ggcgaaggtc cccgggtggc acagcacgat cttcgccccc tacttcgtca
      241 cgggcgcgat cttctccggg atcgccgccg ttgtgatcgt catggccacg ctgcgaaagg
      301 tattccacct ggagcgggtg ctcacggtgg tgcagttcga aaacctcgga aaattgctgt
      361 gcaccatgtc gctcctttgg ggctacttct atttctccga cttcctcacc gtctggtaca
      421 accggaatcc cgaagagtgg gaggtcttcc gctcgtacgc cggcgactac ctccccctgt
      481 tcctcgtcat gatcgggtgc aacttcgtca tctccttccc tctcctttgc atgcgcaggg
      541 tgcgaaggtc gatccccgct ctgagcttcg tctccctcct cgtcgtgatc ggcatgtacg
      601 ccgaacggtt cctgatcgtc gtcccgtcgc tggcgcggcg caacgacccg ttcatctggc
      661 acaactacat cccctcctgg gtggagttct cgatcatggc cggtgcgctg tccatgttcc
      721 tcctgctgta cacgatcttc accaagctct tcccgatcat ggcgatcagc gacatccagg
      781 agcacctctt ccacaccacg gaccggaaga tcggggaagt gactgccgat tcgatcacca
      841 tgccgcacga cacggggaaa ggagggcacg gatgaacggc atcgtggtcg tcggactgtt
      901 cgacgcggtc gatccggcgg cggcggcggc gcgcgccttg cgcgccctcc ccctcccgga
      961 gggggacgta acgaccctct cccccatggc gctcccggac ggagcggtct gcaaggaccc
     1021 gcgcgggatc gggttccccg gattcacgct cgtcggttgg ttcgtcggcg cgctcgcggg
     1081 gcttgccctc gcgctgggga cctatctcct ctacccgctg atcacaggcg ggaaggcgat
     1141 catctccgtt ccccctaccc tcatcgtcac ctacgaggtg gcgatgctgg gcgcgttggc
     1201 ggggacggtc atcgcggggt tccgggagat gggactttgc cggttccgca ccagattgat
     1261 ccacgaccca cggatccagg aagggatgat cctgctgtgc gcgcgggttt ccggggaaga
     1321 gcagacgaga cgggcgatgg aggcgatgaa aagcgcgggc gggatcgacg tgcggcccga
     1381 ggagggggag ctgtgaggag gatctccctc gcccgactca tcgttttcgc cgccgccttc
     1441 ctcgccatgg cgctccttgc aggctgcgag aagctcgacc ggaacatgta cgacaacccg
     1501 gcgttccggc cccaggagga gccggtgcgg cttcccccgg cgggctccgt cccgacgaaa
     1561 ggattggagc acaccccgaa gccggggacc caggaggccg cggccctcaa gaatccggag
     1621 aaggtgaccg agttctccct cctcacgggg aaggagctgt tcggcatcta ctgcacgcct
     1681 tgccatggcg cttcgggcaa gggtgacggt cccgtggcga agaagttcgt gcccactccc
     1741 gcagacatct cgggtacggg atacggcggg aagtaccccg aaggggaact gttcgccgtc
     1801 gtcacccacg gcaagaacgg catgcccccc ttccgctcc
//
LOCUS       DASVJL010000362         4920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_341702, whole genome shotgun sequence.
ACCESSION   DASVJL010000362 DASVJL010000000
VERSION     DASVJL010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341702"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VF357_07680"
     CDS             <1..592
                     /locus_tag="VF357_07680"
                     /inference="COORDINATES: protein motif:HMM:NF014764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEX7524066.1"
                     /translation="DLKQDHRFKSVLIFKNHGAAAGASLTHSHSQLIALPVIPKRVME
                     EMNGCGEYYRFRDRCLFCDIVVQEMEQKVRIVEETGEFLAFSPYAPRFPFETWIVPKR
                     HQCAYEMIEADQAKALAAVFRRTLRRLNLALENPPFNFIVHSAPFQERAVDFFHWHIE
                     IMPKLTKVAGFEWGSGFYINPTPPEESAKYLRELPE"
     gene            605..2065
                     /gene="glgA"
                     /locus_tag="VF357_07685"
     CDS             605..2065
                     /gene="glgA"
                     /locus_tag="VF357_07685"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX7524067.1"
                     /translation="MKVLVASSEIVPFAKTGGLADVTGALPKALRRIGVEADCVLPLY
                     RCVDRDRFPFKGPGQAILVPLGNREEEGSVEETDAGGGVRAFLVRNDRYFDREFLYGT
                     RDGDYVDNSERFTFFCRAVMEWIARSGRRYDIIHCNDWQTALLPVYVKMLYADRQAFR
                     GTGTVFTVHNLGYQGLFWNHDLPMMGLGWELFTPRGLEFYGKINLMKAGLQFADILST
                     VSDTYSREIQTPEYGYGLEGVLYERREDLYGIVNGIDDEEWHPATDRRIAANYSADYM
                     AGKGACRRDLLSVFGLPPGDEPVIGLIGRLTAQKGFDLVERIGEWLARQPLRMVILGA
                     GERKYEETMEELGRKYPDRIAIRVAYDDALAHKIEAGSDMYLMPSRYEPCGLNQIYSL
                     KYGTVPIVRETGGLADTVTDADENPAEGTGFTFHGYETEDLKDAVSRALAAYADRPRW
                     DAIVRRGMARDFSWEASARAYVDLYGKALRKRVPKG"
     gene            2072..4543
                     /locus_tag="VF357_07690"
     CDS             2072..4543
                     /locus_tag="VF357_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7524068.1"
                     /translation="MVENPHTRFSLLARIVEISNSNIQVENRLKHICDFLSRETRSDC
                     VCIYRREARGEDLVPWVSSCIEIDECAMMDFVVRAGEGVSGKAARKRAPVFFPDVKTN
                     PPTLAVPQELRDFTSILSVPVMDDVYLYGVMNFSTLGPTSPTEEDLTFLGFVATEVAG
                     TIRNSRLYNDARKRVSELITLNEIGRAISSTFEVKGTLDYVAKTTMRLLSADGCTVRL
                     TGEGRGVLKVVLDEGYSRPGFRREIRSIGKELALGIHGEKRTLLINGPEDSPLHSSLA
                     GHGVVSFLGLPIVSQGKSLGTISYYSSSPGLRFDMEGVHLMQTVCSQLANMIENASIL
                     RKAQRLAQENQVKVQRISTLYDVARALMSTVKMDRLLPIILYALISPGGLNFSRAILF
                     LTSEDGDGLCARMAMGPRGRREARRIARLPKGLLGDSATVAVEEEIRTLLWSDVGNLR
                     IPMSDVSCLVAKAVKEKRAVRTESGCVAPEDARDAAPPVGFCGSHPPSFAAVPLVTKG
                     EARGVIYVDNLFLGRPITEEDIQLLTMFASNASLAMENASLYESLQGALDIVRTTQGR
                     LVQSEKLMALGEMAARIAHEIKNPLTAIGGFARRIASPRAGAGAHSLERYAQIILKEV
                     QRLERIVNETLYFSREMVSAFRIVNLNAEIRDVLSLFREEFEEARISAVVDLSQDLPS
                     ISADPDQIRQVVWNLVSNAIQAMGGSGILTVATHLADPAGGTGVVLEVSDTGGGIARD
                     VVHNIFNPFFTTKAKGTGLGLPIVHSIVEKHGGTIHLDNREGVGVAFSIFLPLVPKGA
                     GTGDRILEQMRKGGVNGNGPAANRG"
     gene            4512..>4920
                     /locus_tag="VF357_07695"
     CDS             4512..>4920
                     /locus_tag="VF357_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7524069.1"
                     /translation="METVRRRIGDKVEAGERLSEADALALYRTRDLLAVGEMAALANR
                     RANGDRVYFIVNRHVNPTNICVNRCRFCAFSKERNDPLAYTMTMDEILRRAEEAREQR
                     ATELHIVGGLHPDLPFDFYLTMLRSLRERFPSLH"
BASE COUNT          861 a         1583 c         1648 g          828 t
ORIGIN      
        1 cgacctgaag caagaccacc ggttcaagtc ggtcctcatc ttcaagaacc acggggccgc
       61 cgccggggcg tccctgaccc acagccactc ccagctgatc gcccttcccg tcatcccgaa
      121 gcgggtgatg gaggagatga acggctgcgg ggagtattac cgtttccgcg accggtgcct
      181 cttctgcgac atcgtcgtcc aggagatgga gcagaaggtc cgcatcgtcg aggagaccgg
      241 ggaattcctc gccttctcgc cgtacgcccc ccgcttcccg ttcgagacct ggatcgtccc
      301 gaagcgccac cagtgcgcct acgagatgat cgaggcggac caggcgaaag cgctcgccgc
      361 cgtcttccgg cggaccttgc ggcgcctgaa cctcgccctc gaaaacccgc ccttcaactt
      421 catcgtccac agcgcccctt tccaggagag ggcggtggac tttttccact ggcacatcga
      481 gatcatgccg aagctgacga aggtggcggg gttcgagtgg gggtccgggt tctacatcaa
      541 cccgacccct cccgaggagt ccgccaagta cctccgcgag cttccggagt aaggcgctga
      601 tccgatgaag gtcctcgtgg cctcttccga gatcgtcccc ttcgcgaaaa cgggggggct
      661 ggcggatgtt accggggcgc tccccaaggc gcttcgcagg atcggcgtcg aggcagactg
      721 cgtcctcccc ctctaccgct gcgtggatcg ggaccggttc cccttcaagg ggccggggca
      781 ggcgatcctc gtccccctcg gaaaccggga ggaggagggg agcgtcgagg agaccgacgc
      841 gggaggcggc gtccgcgcct tcctcgtccg caacgaccgg tacttcgacc gggagttcct
      901 ctacgggacc cgcgacggcg actacgtcga caactccgag cggttcacct tcttctgccg
      961 cgccgtcatg gagtggatcg cgcgctccgg gcggcggtac gacatcatcc actgcaacga
     1021 ctggcagacc gccctcctcc cggtgtacgt gaagatgctc tatgccgatc gccaggcgtt
     1081 ccgcgggacg ggaaccgtct tcaccgtcca caaccttggg taccaggggc tgttctggaa
     1141 ccacgatctt ccgatgatgg gtctcgggtg ggagctgttc accccgcggg ggctggagtt
     1201 ctacgggaag atcaacctga tgaaggcggg cctgcagttc gcggacatcc tctccaccgt
     1261 gtccgacacc tatagccgcg agatccagac cccggaatac gggtacggtc tcgagggggt
     1321 cctctacgag cgccgggagg acctttacgg catcgtgaac gggatcgacg acgaagagtg
     1381 gcacccggcc accgaccggc ggattgcggc gaactactcc gcggactaca tggcggggaa
     1441 aggggcgtgc cggcgggacc tgctctccgt gttcgggcta cctccgggcg acgaacccgt
     1501 cattggtctg atcgggcggc tcacggcgca aaaagggttc gacctcgtcg agcggatcgg
     1561 ggagtggctc gcgagacagc ccctgcggat ggtgatcctc ggcgccgggg aacggaagta
     1621 cgaggagacg atggaggagc tcggccggaa ataccccgac cggatcgcga tccgcgtggc
     1681 gtacgacgac gcgctggcgc acaagatcga ggcagggtcg gacatgtacc tgatgccctc
     1741 ccggtacgaa ccgtgcgggc tgaaccagat ctacagcctg aaatacggca ccgtccccat
     1801 cgtgcgggag acgggcggcc tggcggacac ggtgacggac gctgacgaaa accccgccga
     1861 ggggaccggg ttcaccttcc acgggtacga gacggaggat ctcaaggatg ccgtctcgag
     1921 ggcgttggcg gcgtacgcgg accgtccccg ctgggatgcg atcgtccggc gggggatggc
     1981 gcgggacttc tcctgggagg cgtcggccag ggcatacgtc gacctttacg ggaaggcgct
     2041 gcgcaagcgg gtcccgaagg ggtagcctcc catggtggag aacccgcaca cccgcttttc
     2101 cctcctggcc cggatcgtcg agatctccaa ctcgaacatc caggtcgaga accgcctgaa
     2161 gcacatctgc gattttctct cccgggagac ccgttccgat tgcgtctgca tctaccggcg
     2221 cgaagcgcgc ggggaggacc tcgtgccgtg ggtctcctcg tgtatcgaga tcgacgagtg
     2281 cgcgatgatg gacttcgtcg tccgggcggg cgaaggggtt tccgggaagg ctgcccggaa
     2341 gcgcgccccg gtcttcttcc ccgacgtgaa gacgaatccc ccgacgctcg ccgttcccca
     2401 ggaattgcgc gacttcacct ccatcctctc cgtgccggta atggacgacg tgtacctcta
     2461 cggagtgatg aacttctcca ccctgggtcc cacgagtccc acggaggagg acctgacgtt
     2521 cctcgggttt gtggcgacgg aggtggcggg gacgatccgc aacagccgtc tttacaacga
     2581 cgcccgcaaa cgcgtctcgg aactcatcac gttgaacgag atcggacgcg cgatctcctc
     2641 gaccttcgaa gtgaagggaa cgctggacta cgtcgccaag acaacgatgc ggctgctgtc
     2701 ggcagacggg tgcacggtgc gccttacggg ggaggggcgc ggcgttctca aggtcgtgct
     2761 ggacgagggg tacagccgcc ccggcttccg ccgtgagatt cggtcgatcg gaaaggagct
     2821 ggctctgggg atccacggag agaagcgcac gctcctgatc aacggtcccg aggattcgcc
     2881 gctccattca tcgcttgccg gccacggggt cgtctccttc ctcggcctcc ccatcgtttc
     2941 ccaggggaaa tccctcggga cgatcagcta ctactcctcc tcccccgggc tgcgattcga
     3001 catggaaggg gtgcacctga tgcagacggt ctgctcccag ctggcgaaca tgatcgagaa
     3061 cgcgtcgatc ctccggaagg cgcagcggct ggcgcaggag aaccaggtca aggtgcagcg
     3121 gatctcgacg ctctacgacg tcgctcgcgc tttaatgtcc acagtcaaga tggatcggct
     3181 gcttccgatc attctgtacg ccctcatctc cccgggcggg ctgaacttca gccgggcgat
     3241 cctgttcctt acctcggagg acggggacgg gctttgcgcc cggatggcga tggggccgcg
     3301 cggtcgccgg gaggcgcggc ggatcgcccg gcttccaaaa gggctgctgg gggacagcgc
     3361 aaccgtggcg gtggaggagg agatccggac cctgctctgg tccgacgtcg ggaacctgag
     3421 gatcccgatg tcggacgtct cctgtctcgt ggcgaaggcg gtgaaggaga agcgggcggt
     3481 gcggacggag tccggctgcg tggccccgga ggacgctcgg gatgcggctc ccccggtggg
     3541 gttctgcggc agccatccgc cctccttcgc cgccgttccc ctggtgacca agggggaggc
     3601 gcgcggggtg atttacgtgg acaacctctt cctggggaga ccgatcaccg aagaggacat
     3661 ccagctgctc acgatgttcg cctccaacgc gtccctcgcg atggaaaacg cctccctcta
     3721 cgaatcgctt cagggcgcgc tcgacatcgt ccggaccacg cagggccggc tggtgcagag
     3781 cgagaagctc atggcgctcg gggagatggc ggccaggatc gcccacgaga tcaagaaccc
     3841 cctcaccgcg atcggcgggt tcgcacggag gatcgcctcc ccccgggccg gggccggggc
     3901 gcattcgctc gagcggtacg cgcagatcat cctgaaggag gtgcagcgcc tggagcggat
     3961 cgtcaacgaa acgctctatt tctcccggga gatggtctcc gcgttccgca tagtaaacct
     4021 gaacgcagag atccgcgacg tgctgtcgtt gttccgggag gagttcgagg aggcccgcat
     4081 ctccgcggtg gtcgacctct cccaggatct tccctccatc tcggcggatc cggaccagat
     4141 tcggcaggtg gtatggaacc tcgtttccaa cgccatccag gcgatggggg ggagtggcat
     4201 cctcaccgtc gccacgcacc tcgccgaccc cgcggggggg accggagtgg ttctcgaggt
     4261 gagcgacacc ggcggcggga tcgcgcgcga cgtggtgcac aacatattca acccgttctt
     4321 caccacgaag gcgaagggga ccggcctcgg gcttcccatc gtccactcca tcgtggagaa
     4381 gcacgggggg acgatccatc tggacaaccg ggagggtgtc ggtgtagcct tctccatctt
     4441 cctccccctc gttccgaagg gggcggggac cggggaccgg atcctcgagc agatgcgcaa
     4501 aggcggagtg aatggaaacg gtccggcggc gaatcgggga taaggtcgag gcgggggagc
     4561 ggctgtcgga ggccgacgcg ctcgcgcttt atcgcacgcg ggacctcctc gccgtcgggg
     4621 aaatggcggc cctggcgaac cgacgggcga acggcgaccg cgtctacttc atcgtcaacc
     4681 ggcacgtgaa cccgacgaac atctgcgtga accggtgcag gttctgcgca ttcagcaagg
     4741 agaggaacga tcctctcgcg tacacgatga cgatggacga gatcctccgc cgggcggaag
     4801 aggcccggga gcagcgggcc acggaacttc acatcgtcgg gggattgcat ccggacctcc
     4861 ccttcgactt ctacctgacg atgctgcgat ccctgcggga acggttccct tctctgcacg
//
LOCUS       DASVJL010000363         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_998569, whole genome shotgun sequence.
ACCESSION   DASVJL010000363 DASVJL010000000
VERSION     DASVJL010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998569"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VF357_07700"
     CDS             <1..420
                     /locus_tag="VF357_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX7524070.1"
                     /translation="YELSQFLVDVLGVTDPKSNFSGKVTYHDSCHLRRGLGVIEAPRM
                     LLRAVPGVEFVEMEESDRCCGFGGVFSLKYPQISCKMTERKVERILATGASYVTSGDL
                     GCLLNIGGLISRIGYPVKAIHLAEILAGKTAGRGDAG"
     gene            420..1832
                     /locus_tag="VF357_07705"
     CDS             420..1832
                     /locus_tag="VF357_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEX7524071.1"
                     /translation="MELNANAFGRNAMKALLDKTLQEALGRTTGRFQAHRDAAAAAFP
                     EFEATRERASRIKRDVLDHLDTYLARFIEEAEKRGAIVHVARDAVQAREIAARIARDE
                     GITLAVKSKSMASEEVSFNEALQEAGVTVVESDLGEFIIQLAGEAPSHIIAPAVHKTR
                     EEISRLFERHLGEPRTDSIPELVGMARKHLRSKFLSAGMGVSGGNFLVADTGSVVLVT
                     NEGNGRMGTVLPRVHLAVVGIEKVIPRMADLPTFLRLLPRSASGQTISSYVSIVTGTR
                     RAGDPEGPERMHILLLDCGRSAILEGKYREILKCVRCAACLNSCPVYQSVGGHPYGWV
                     YSGPIGAVLTPLLVGLPEAAALPDASTLCGACADVCPVKIPLPDFLLELRSDAREQGL
                     KTPGEIAAMKGYAGVMKRAGLLEAIERIAGVLGQFFTKGKGIGGLPYPFSRWTERRDF
                     PAPAAQPFRKQWKIRRGVTR"
     gene            1829..>2146
                     /locus_tag="VF357_07710"
     CDS             1829..>2146
                     /locus_tag="VF357_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524072.1"
                     /translation="MNERESLFRRLSAACGGHDGAAVKEPLSCGEPMEPEGDRVALFA
                     KTFTAAGGVLLRGPVEKALSDLGEMLRAEGVTALFFPEDDAAARGIAEALVPFGPFNL
                     TTGV"
BASE COUNT          339 a          697 c          777 g          333 t
ORIGIN      
        1 tacgaactct cccagttcct cgtcgacgtc ctcggcgtga ccgacccgaa atcgaatttt
       61 tccggaaaag tgacgtacca cgactcgtgc cacctgcggc gcgggctcgg ggtgattgaa
      121 gcgccgcgga tgctgctgcg cgccgttccc ggcgtcgagt tcgtcgagat ggaggagagc
      181 gaccggtgct gcggcttcgg gggcgtcttc tccctcaagt acccgcagat ctcctgcaag
      241 atgacggagc gcaaggtcga gcggatcctc gcgaccggcg cgtcgtacgt cacctccggg
      301 gacctcgggt gcctgctgaa catcggaggg ttgatctcgc ggatcggata cccggtgaag
      361 gcgatccatc tcgccgaaat cctcgcgggg aaaaccgccg ggagagggga cgccggctga
      421 tggagctcaa cgccaacgcg ttcgggcgaa acgccatgaa agcgctcctc gacaagacgc
      481 tgcaagaagc tctcgggcgc accacggggc ggttccaggc ccaccgcgac gcggcggccg
      541 ccgcctttcc ggagttcgag gcgacccgtg agcgggcgtc gcggatcaag cgggacgtcc
      601 tggaccatct ggacacgtac cttgcgcgct tcatcgagga ggcggaaaaa cggggcgcga
      661 tcgtccatgt ggcccgggac gcggtccagg cgcgggagat cgcggcgcgg atcgcgcggg
      721 acgaggggat caccctcgcc gtgaagtcga agtcgatggc gtccgaagag gtgtccttca
      781 acgaggcgct gcaggaagcg ggcgtcacgg tcgtcgagtc cgacctcggg gagttcatca
      841 tccagcttgc gggggaggcg ccttcccaca tcatcgcccc cgccgtccac aagacgcgcg
      901 aagagatctc ccgcctcttc gagcgccacc tcggggagcc gcggaccgac agcatccccg
      961 agctggtcgg gatggcccgc aagcatctgc ggtcgaagtt cctctccgcg ggaatggggg
     1021 tctccggcgg gaacttcctg gtggccgaca ccggctccgt ggtgctcgtc accaacgagg
     1081 gaaacgggcg gatggggacg gtcctgccgc gcgtccacct cgccgtcgtc gggatcgaga
     1141 aggtcatccc gcgcatggcg gacctcccga ccttcctgcg cctgctgccg cgcagcgcct
     1201 ccggccagac gatctcctcg tacgtgtcga tcgtgacggg aacgaggcgc gcgggcgacc
     1261 ccgaggggcc ggagcggatg catatcctcc tgctcgattg cggccgcagc gcgatcctcg
     1321 aggggaaata ccgggagatc ctgaaatgcg tccgctgcgc cgcatgcctg aactcctgtc
     1381 ccgtctacca gagcgtgggg gggcacccct acgggtgggt ctactccggt ccgatcggcg
     1441 cggtcctgac cccgctgctg gtcggcctgc ccgaagcggc cgcgctgccc gacgcgagca
     1501 cgctgtgcgg cgcgtgcgcg gacgtttgcc cggtgaagat cccgctgccg gacttcctgc
     1561 tcgagctgcg gtccgacgcg cgggaacagg gcttgaagac tcccggcgag atcgccgcga
     1621 tgaaagggta cgcgggggtg atgaagcggg ccgggctgct cgaagcgatc gagcggatcg
     1681 ccggggtcct ggggcagttc ttcacaaagg ggaagggaat cggcggactg ccgtacccct
     1741 tctcgaggtg gacggagcgc cgcgacttcc ccgcgccggc cgcgcagccg ttccgcaagc
     1801 agtggaagat ccggcggggg gtgacgcggt gaacgagcgg gagtcgctgt tccgccggct
     1861 ttccgcggcg tgcgggggtc acgacggcgc cgcggtcaag gagccgcttt cctgtggaga
     1921 gcccatggag ccggaaggag accgcgtcgc gctgttcgcg aagacgttca ctgccgccgg
     1981 cggtgttctc cttcgcgggc cggtggaaaa ggcgttgtcc gaccttgggg aaatgttgcg
     2041 ggcggagggg gtgaccgccc tcttcttccc ggaagacgac gcggccgcgc gcgggatcgc
     2101 ggaggcgctc gtcccgttcg ggccgttcaa cctcacgacg ggagtg
//
LOCUS       DASVJL010000364         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1749684, whole genome shotgun sequence.
ACCESSION   DASVJL010000364 DASVJL010000000
VERSION     DASVJL010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749684"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..772
                     /locus_tag="VF357_07715"
     CDS             98..772
                     /locus_tag="VF357_07715"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7524073.1"
                     /translation="MTRPPVRRGGFTLLEILLALSVLAVLLLLLLSAFTGAARVRETL
                     SSRARDFRQIRLVLDRAGTDLMCAFAATSREESALSLREDQLSGMPAATLIFTAFQLF
                     DGDSGHPPAEIVKIRYFPRIGADGVTLELHREQADLPFIGNKIPLRESMVADGLRGFR
                     VELYNGTAWVKEWPAGGGSKTTLPKKAAVTLVDARGDIYRREIPILLAGQEGNVILSG
                     RRPGKP"
     gene            769..>1517
                     /gene="gspK"
                     /locus_tag="VF357_07720"
     CDS             769..>1517
                     /gene="gspK"
                     /locus_tag="VF357_07720"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEX7524074.1"
                     /translation="MTRRRENEKGIALLVTLLILVLVVALAHEVFRIGAQSAQTGAYG
                     RDSIRCILLAEGGTGAARIALRVDARDNQYDTLDEIWSRNALPITLGDGEVLVTIEDE
                     ERKIDLNRLMLPNGNAPDEKRLAVFQRLLDTLGIDRAVADAVVDWLDNDENPRVGGAE
                     SPYYLGLPNPYQAKNDLFDTIGELRLVRGVTSEVFEKLRPFVTVSSSGMVNINTAPKE
                     VLMSLSAGTDLAVGAIDAKTADEIIEYRKDHP"
BASE COUNT          255 a          518 c          508 g          236 t
ORIGIN      
        1 cggccatgca tcccgacgcc cgggaagtcc acgtcaccgt cacctggtcc tccgaaggcc
       61 gcgaggagca ggtctccctc gcaggcgtcg ccatcaaatg acgcgaccgc ccgttcgccg
      121 cgggggattc accctcctgg agatccttct cgccctgtcg gtgctcgcgg tcctccttct
      181 gctcctgctc tcggcgttca cgggggcggc cagggtgcgc gagacgctct cctcgcgggc
      241 gcgggatttt cgccagatcc gccttgtcct cgaccgggcg gggaccgacc tcatgtgcgc
      301 tttcgcggca acgtcgcggg aggagtccgc gctctccctg cgcgaggatc aactctccgg
      361 aatgccggcg gcgacgctca tcttcacggc cttccagctt ttcgacgggg atagcggtca
      421 cccgcccgcg gaaatcgtca agatccggta tttcccgaga atcggagccg acggcgtgac
      481 cctcgaactg caccgggagc aggccgacct cccgttcatc gggaacaaga tccccctcag
      541 ggaatccatg gtggcggacg ggttgcgggg cttccgcgtc gagttgtaca acgggacggc
      601 gtgggtcaag gagtggcccg cgggcggggg ctcgaagacg acgctgccga agaaagccgc
      661 cgtcacgctg gtggacgcgc gcggggacat ctaccgccgt gagatcccga tcctcctggc
      721 cggccaggag ggaaacgtga tcttgtccgg gcggcgtccg ggaaaaccat gacccggcgg
      781 cgcgaaaacg agaaggggat cgccctcctc gtcaccctcc tgatcctggt gctcgtcgtc
      841 gccctcgccc acgaggtctt ccgcatcggt gcgcagtcgg cccagaccgg cgcgtacggg
      901 agggattcca tccgctgcat cctgctcgcc gaaggcggga ccggcgcggc gcggatcgcc
      961 ttgcgggtcg acgcgcggga caaccagtac gacaccctcg acgagatctg gtcacggaac
     1021 gccttgccga tcacgctcgg ggacggggaa gtcctggtca ccatcgagga cgaggaacgg
     1081 aagatcgacc tgaaccggct catgctgccg aacgggaacg cgccggacga gaagcgcctc
     1141 gccgtgttcc agcgtctcct ggacaccctc gggatcgacc gggccgtggc ggacgccgtc
     1201 gtcgactggc tggacaacga cgagaatccc cgcgtcggcg gggcggagag cccgtactac
     1261 ctcggccttc cgaacccgta ccaggcgaag aacgacctgt tcgacacgat cggggagctg
     1321 cgcctggtgc gcggcgtgac cagcgaggtc ttcgagaagc tgcgtccctt cgtgaccgtc
     1381 tcctcctccg gcatggtgaa catcaacacc gcgccgaagg aggtgctgat gtccctctcg
     1441 gccggaacag acctggcggt tggggcgatc gacgcgaaga ccgcggacga gatcatcgaa
     1501 taccggaagg accaccc
//
LOCUS       DASVJL010000365         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_529918, whole genome shotgun sequence.
ACCESSION   DASVJL010000365 DASVJL010000000
VERSION     DASVJL010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529918"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1834
                     /locus_tag="VF357_07725"
     CDS             <1..>1834
                     /locus_tag="VF357_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7524075.1"
                     /translation="LRRRVEEMVDGVGRLSVTTFHSFCARVLRSFPAEAGVDPLFEVL
                     PETAAADAWDAGFRKFLQGEFGGEEVPPEWERILSRLPDPSKSWSVIRRLCLFQRDLL
                     RGPAADFGSPGDFLDFLVREYVSSVEYFRSFVDGIRAPADDPAAERFREALRVLDSAW
                     EAVSRGDLAPAEASAKEGATVFALDLRKAASKKKFPRPEGRKLAEVRDALLRFWKQVG
                     EVPAGDAAARFLVARAGAALASFEAAKGSGLDFMDLLLRASDLLVENPAVARRLGERF
                     RHIFVDEFQDTDPLQAGVLRAIAADAVPGHLFVVGDPKQSIYGFRRADIQVYRKFREG
                     MVDAGGEDVSLTRNFRSRPDLLATLSGLFSRVLSGGEDFSPAYAHVKADRKDPGEGHP
                     VTLYRLDPAVDEAEFLAALVGRIVGSAKVRDRCGVERPASFRDIAVLYRSDASGDVLS
                     GYRAALAAAGIPHVVPSRKGFFLRQEIQDLRMVLSAVDVPADRSARHAAMKTIFFGLS
                     DEEILPLYGEDPAAVPARAREAVALLSRLSARRGRASLPDLLAALYGETGVDFVAARI
                     PDGERIVRNLSKAAEMARAFEWGGAGSLNLFLAEIRRKADEGRE"
BASE COUNT          227 a          619 c          672 g          316 t
ORIGIN      
        1 cgctgcgtcg gcgggtcgag gagatggtcg acggcgtggg ccgcctctcc gtgacgacgt
       61 tccactcgtt ctgcgcccgc gttctccgct ccttcccggc ggaggcgggg gtcgaccccc
      121 tcttcgaggt gctcccggaa accgcggcgg cggacgcgtg ggacgccggg ttccgcaaat
      181 ttctgcaggg ggagttcggg ggggaggagg tccccccgga gtgggagcgg atcctctccc
      241 gccttcccga tccctcgaag tcgtggtcgg tgatccgccg cctctgcctc ttccagcgcg
      301 acctgctgcg cggcccggcg gcggatttcg ggtccccggg ggacttcctc gacttcctgg
      361 ttcgggaata cgtttcttcc gtggagtatt tccggtcctt cgtcgacggg atccgggctc
      421 ccgccgacga cccggcggcg gagaggttcc gcgaagccct ccgcgtcctc gactccgcgt
      481 gggaagcggt ctcgcggggg gatctcgccc cggcggaggc gagtgcgaag gaaggggcga
      541 cggtttttgc gctggacctg cgcaaggcgg cgagcaaaaa aaagttcccc cgccccgagg
      601 ggcggaaact ggccgaggta cgcgacgcgc tgctccgttt ctggaaacag gtgggcgagg
      661 tccccgccgg ggacgcggcg gcgcgcttcc tcgtagcgcg ggccggcgcc gcccttgctt
      721 cgttcgaggc ggcgaagggg agcgggctcg acttcatgga cctgctgctg cgggcgagcg
      781 accttctcgt ggagaatccc gcggtcgcgc gacggctcgg ggagcggttc cgtcacatct
      841 tcgtggacga gttccaggac acggaccctt tgcaggcggg ggttcttcgg gcgatcgccg
      901 ccgacgcggt gccggggcac ctcttcgtcg tcggggatcc gaagcagtcg atctacggct
      961 tccgccgcgc cgacatccag gtgtaccgga agttccgcga agggatggtc gacgccggag
     1021 gggaggacgt ttcgctgacc cgcaacttcc gcagccgccc cgacctgctc gcgacgctct
     1081 ccggcctgtt ctcccgcgtc ctctcgggcg gggaggattt ctcccctgcg tacgcgcacg
     1141 tcaaggccga caggaaggat cccggggagg ggcaccccgt cacgctctac cggcttgacc
     1201 ccgccgtgga cgaggcggag ttcctcgcgg cgctggtcgg gcggatcgtc ggatcggcga
     1261 aggtccggga tcgctgcggc gtcgaacgcc ccgcgtcgtt ccgggacatc gccgtcctct
     1321 accgctccga cgcctcggga gacgtgctgt cggggtatcg cgcggcgctc gcggccgcgg
     1381 gaatccccca cgtcgtccct tcgcgaaagg ggttcttcct gcggcaggag atccaggatt
     1441 tgcggatggt gctctccgcc gtcgatgtcc ccgccgaccg gtcggcgcgg cacgctgcca
     1501 tgaagacgat cttcttcggt ctttcggacg aggagatcct ccctctctac ggggaagacc
     1561 cggccgcggt gccggcccgc gcgcgcgagg ccgtggcgct cctttcccgc ctctccgcgc
     1621 ggcggggccg ggcgtcgctt ccggaccttc tcgcggcctt gtacggggag acgggggtcg
     1681 atttcgtcgc ggcgcggatt cccgacgggg aacggatcgt ccggaatctg tcgaaggccg
     1741 cggagatggc cagggccttc gaatggggag gcgccgggtc cctcaatctc tttctcgcgg
     1801 agatccgccg caaggccgac gagggtcggg agga
//
LOCUS       DASVJL010000366         5849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_716846, whole genome shotgun sequence.
ACCESSION   DASVJL010000366 DASVJL010000000
VERSION     DASVJL010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716846"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VF357_07730"
     CDS             <1..861
                     /locus_tag="VF357_07730"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7524076.1"
                     /translation="LTGESGTGKEVIARSVHSMSGRSAAPFLAVNCASLPDTLFESEL
                     FGHERGAFTGAVRREIGLFEAAGGGTIFLDEIAEIKLETQAKLLRALQEKEIRRIGGK
                     ENISVDVRIVAATNRDLDDAVREGKFRADLFYRLNIVKLTLPPLRDRISDIPLLSEHF
                     LAKHGEKGVPPVREITKEAMRLLMRYTWPGNIRELSSVIERAVVLAEGGKIDPGELPL
                     ELREGADAVPARAFDLPPQGVEFEKVEEHLLRQAVGRSGGVHTRGAELLRMSYKAYIY
                     RLKKFGILPP"
     gene            complement(882..1439)
                     /locus_tag="VF357_07735"
     CDS             complement(882..1439)
                     /locus_tag="VF357_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524077.1"
                     /translation="MSGPETPGNRSLIQRRYVFGLLLRLCVNFAGGVGILFVILYFAL
                     SRPLPGDYAGVFHALRNLSPFLRPILAVSVLVYALLTFGSTSALCVYILHKVAGPLYR
                     MEMVLDQYRTGAPTRTVSFRNGDQIQVLAQAFNLWIGTLRRDRQRWLATMKNAERHCL
                     QDDATCRAEMEEALREVAEDMARYR"
     gene            complement(1436..1918)
                     /locus_tag="VF357_07740"
     CDS             complement(1436..1918)
                     /locus_tag="VF357_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524078.1"
                     /translation="MLAFAAIIATGGAIFWKLVDGEFQRVFLLTAIRGHYTFDSAYDI
                     VRDLLARHLAGLFVGVFLAGSVLVLLLVAATRYGIGKAVDSLRAAADGDLSTPTGKGL
                     IGEFDRFGENIDVARSDTLASIMQIRSETTALAAGGISPEEFRLRWDDLKQRIRRIAP
                     "
     gene            complement(1987..2436)
                     /locus_tag="VF357_07745"
     CDS             complement(1987..2436)
                     /locus_tag="VF357_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX7524079.1"
                     /translation="MRIRGVRIRDGLLAALAGVLCFAAPALFASDGPRRGNPHAHFRN
                     PDQCPKCHLTQGSQPVPGRFSTDANAVCLECHAKESMGRSHPVDVRPEEKYRKMKVPA
                     DLRLDDDGRIMCLPCHTAHGQYVSYFLRRSSPDRGFDILCEACHGKR"
     gene            complement(2452..3576)
                     /gene="serC"
                     /locus_tag="VF357_07750"
     CDS             complement(2452..3576)
                     /gene="serC"
                     /locus_tag="VF357_07750"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX7524080.1"
                     /translation="MKRTINFNAGPAALPLPALERARDEFLDFAGSGMSVMEHSHRGK
                     EYESVHDEAIALVRELLGVPVSYEVLLLQGGATALFAQIPMNFLEKGKTARYVVTGAW
                     GQKALGEAKLVAGMYGAETAACNLGVGEGKEKSYTRVPAPSEVKAEAGDAYLHITSNE
                     TIHGVEYNVDPSRAFPATGAVPLIADMSSDFLWRPFDVTKFGMVYAGAQKNIGPSGVV
                     VAVVSKELLDRGRKDIPKIFQFRTHAENKSLYNTPPTFGVYMVRNVLSWLKGQGGLAG
                     MEKINRKKAARLYAVIDGNAGYFRSPVERESRSVMNVVFRLPTPELEERFIAESKKQG
                     MIGLKGHRSVGGIRASIYNAVPYEWVESLAAFMEAFARAK"
     gene            3795..5369
                     /gene="serA"
                     /locus_tag="VF357_07755"
     CDS             3795..5369
                     /gene="serA"
                     /locus_tag="VF357_07755"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX7524081.1"
                     /translation="MKVLALDNLQKVGIDVFVKEGIEVDVKGKMTPEELTAAINQYDA
                     VVVRGATKATAACFENASRIKVIGRAGSGTDNIDKIAATKKGVVVMNTPGGNTVTTAE
                     HAVSMMMALSRQIPQATASMKAGKWEKNKFMGTEITDKVLGVVGLGAIGKVVADRALG
                     VKMVVVAFDPYVSKEDAALLGVETATLDELYARADYITYHTPLTQETKGMVNAASIAK
                     MKDGVRIINCARGALVNEADLLAALQSGKVAGAALDVFATEPPPPDMPLLAHPNVILT
                     PHLGAATTEAQEKVAVLIAEQICDFLKKGTIRNSVNFPSVAGELLPVLKPFLALAERL
                     GAFHGQLLRNPVRELKVDYFGEVGKLSTAPVTISVLKGLLQYQTEEVNLVNARMVAEE
                     RGIKVSESKTPKSEPYASLLKVTVVTKEGESSVAGTVFGGTPRIVQIDAFAIVAELSG
                     GILMLRNQDVPGVVGRIGTFLGEKGINIAGLRLGRTEVGGTAVSLINVDNAVPENVLA
                     QLRKLPNIMDAQYLIF"
     gene            5389..>5849
                     /locus_tag="VF357_07760"
     CDS             5389..>5849
                     /locus_tag="VF357_07760"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX7524082.1"
                     /translation="MKSVIVLGAQWGDEGKGKIVDIYSEFADTIVRPTGGNNAGHTLV
                     VKGEKFVFHLIPSGILHPGKKCVIANGVVIDPKVLLMEIDRLKERGYLKDDAQLKIGL
                     LANIILPYHIRLDKAREEKLGNRKIGTTARGIGPCYEDKIARIGIRACDLVR"
BASE COUNT         1061 a         1866 c         1963 g          959 t
ORIGIN      
        1 ctcaccggcg agagcggcac ggggaaggag gtgatcgccc gcagcgtcca ttccatgagc
       61 ggccgcagcg ccgccccgtt cctcgcggtc aactgcgcct cgctcccgga caccctgttc
      121 gagagcgagc tgttcggcca cgagcgcggg gcgttcaccg gcgcggtgcg ccgggagatc
      181 ggtcttttcg aggccgcggg aggggggacg atcttcctcg acgagatcgc cgagatcaag
      241 ctggagaccc aggcgaagct gctgcgcgcc ttgcaggaaa aggagatccg ccggatcggc
      301 ggcaaggaga acatctccgt cgacgtacgg atcgtcgcgg ccacgaaccg ggatctcgac
      361 gatgccgtgc gggaagggaa gttccgggcg gacctcttct atcggttgaa catcgtgaag
      421 ctcaccctcc cgcccctgcg cgatcggatc tccgacatcc ccctcctttc ggagcacttc
      481 ctcgcgaagc acggcgagaa gggtgttcct ccggtgcgcg agatcacgaa ggaggcgatg
      541 cggctcctga tgcggtacac gtggcccggg aacatccgcg aactctcctc cgtcatcgag
      601 cgggcggtcg tcctcgcgga aggcgggaag atcgaccccg gggagctgcc gctggaactg
      661 cgggagggtg cggatgccgt ccccgcgagg gcgttcgacc ttcctcccca aggggtggaa
      721 ttcgagaagg tcgaggagca cctgctgcgc caggcggtgg ggcgttccgg gggggttcac
      781 acccgggggg ccgagctgct ccggatgagc tacaaggcgt acatctaccg gttgaagaag
      841 ttcgggatcc ttcctccctg atccccccgc ttctcttttt tttaacgata tctcgccatg
      901 tcttccgcga cctcccggag cgcctcttcc atttccgcgc ggcacgtcgc atcatcctgg
      961 aggcagtgcc gttccgcgtt cttcatcgtg gccagccacc gttggcggtc gcggcggagc
     1021 gttccgatcc acaggttgaa cgcctgcgcg agcacctgga tctggtcgcc gttccggaag
     1081 gaaactgtcc gcgtgggggc gcccgttcga tactggtcca ggaccatttc catccggtat
     1141 aacggccccg ccaccttgtg gaggatgtag acgcaaagcg ccgacgtcga gccgaaggtc
     1201 agcagcgcgt aaaccaggac ggagacggcg aggatcggcc ggaggaacgg ggagaggttg
     1261 cgcagggcgt gaaagacccc cgcgtagtcc ccggggaggg gccgggagag cgcgaagtag
     1321 aggatgacga acaggatgcc cacccccccc gcgaagttga cgcagagacg cagcagaagg
     1381 ccgaaaacat accggcgctg gatcaaggaa cggtttcccg gggtttcggg gccgctcatg
     1441 gggcgatcct ccggatccgc tgcttcaaat cgtcccagcg gagccggaac tcctccgggg
     1501 aaatcccgcc cgcggcgagg gccgtcgtct cgctccggat ctgcatgatg gaggcgagag
     1561 tgtcggagcg ggcgacgtcg atattttccc cgaaccggtc gaactcgccg atgagaccct
     1621 tgccggtggg cgtggacagg tccccatccg ccgccgcccg cagagagtcg acggccttgc
     1681 cgatgccgta acgagtggcg gcgacaagga gcaggaccag gacggaaccg gccagaaaga
     1741 ccccgacgaa gagacccgcc aagtgccggg cgagaagatc ccggacgatg tcgtacgcgg
     1801 agtcgaacgt gtagtgtccc cggatggcgg taagcaggaa cacccgctgg aactcgccgt
     1861 cgaccagttt ccagaagatc gcgcccccgg tcgcgatgat ggccgcgaac gccagcatcg
     1921 ccggtccctg gaagcggggg tttacgtagc gggtcccggg ccgaggcgga tgcaacgggg
     1981 ggggcttcat ctttttccgt ggcacgcttc gcaaagaata tcgaagccgc ggtccgggct
     2041 cgacctgcgc aggaagtacg acacgtattg gccgtgcgcc gtgtggcagg gcaggcacat
     2101 gatccggccg tcgtcatcca gccggagatc ggcaggcacc ttcatcttcc ggtacttctc
     2161 ttccgggcgc acgtccacgg ggtgggagcg ccccatgctt tccttcgcgt ggcattcaag
     2221 gcagaccgca ttcgcgtcgg tcgagaaacg gccgggaacc ggctgcgaac cctgggtgag
     2281 atggcacttc gggcactggt ccgggttccg gaaatgcgcg tgagggtttc cgcgccgggg
     2341 tccgtcggag gcgaaaaggg ccggggcggc gaagcagaga acgccggcca gggccgccag
     2401 cagtccatcc cggatgcgta cgccgcggat cctcatcgcc tccggcgcct ctcacttcgc
     2461 ccgcgcgaac gcctccatga aggcggcgag cgactccacc cattcgtacg gaacggcgtt
     2521 gtagatcgaa gcccggatcc ccccgacgga gcggtgcccc ttgaggccga tcatcccctg
     2581 cttcttcgac tcggcgatga accgctcctc gagctcggga gtcgggagac ggaagacgac
     2641 gttcatcacc gagcggctct cccgctccac cggcgagcgg aaatatcccg cgttgccgtc
     2701 gatgacggcg tagaggcgcg ccgccttctt ccgattgatc ttctccatcc cggcgagccc
     2761 cccctgtccc ttgagccagg agaggacgtt gcggaccatg tagacgccga aggtgggtgg
     2821 cgtgttgtag agcgacttgt tctcggcgtg ggtgcggaac tggaagatct tcgggatatc
     2881 cttccgcccc cggtcgagga gttccttcga caccaccgcg accaccacgc ccgacggacc
     2941 gatgttcttc tgggcgcctg cgtagaccat gccgaacttc gttacgtcga acgggcgcca
     3001 gaggaagtcg ctcgacatgt ccgcgatcag cggcacggcc cccgtggccg ggaaggcgcg
     3061 tgacgggtcg acgttgtact cgacgccgtg gatcgtctcg ttggaggtga tgtgcaggta
     3121 ggcgtcaccc gcctcggcct tcacctcgga gggcgccggc acgcgggtgt agctcttctc
     3181 cttcccttcg ccgaccccga ggttacaggc ggccgtctcg gcgccgtaca tccccgcgac
     3241 gagcttcgcc tcgcccagcg ccttctggcc ccaggcgccg gtgacgacgt accgggccgt
     3301 cttccccttt tcgaggaagt tcatggggat ctgtgcgaag agcgcggtgg ctcctccctg
     3361 gagcagcagg acttcgtagg atacgggtac gccgagcagc tctcgaacga gggcgatcgc
     3421 ctcgtcgtgg accgactcgt attctttccc ccggtggctg tgctccatga ccgacatgcc
     3481 gcttccggcg aagtcgagga actcgtcgcg ggcgcgctcc agcgcgggaa ggggaagggc
     3541 ggcgggtccg gcgttgaaat tgattgtccg tttcatgggg tgtcctcgca tgggcgcgcg
     3601 ggggattccc gcgggacgcg ccgcgttgcc ggccgcgggc cgaatttcaa tcccccgcat
     3661 tctcgcaagg tccgcgcccg ccttcaaggg ttttctgcgc ttccagagat tgacttttcc
     3721 tcatcccttt gataggtaat gcagttgcat ttcccccgga ctacccacga accggaaaac
     3781 aggagaagac acggatgaaa gtcctcgcgt tggacaacct gcagaaggtc ggcatcgacg
     3841 tattcgtgaa ggaagggatc gaggtcgatg tgaaggggaa gatgacgccc gaagagctga
     3901 cggcggccat caaccagtac gacgccgtcg tcgtccgcgg cgccaccaag gccacggcgg
     3961 catgcttcga gaacgcctcg cggatcaagg tgatcgggcg cgccggcagc ggtacggaca
     4021 acatcgacaa gatcgccgcc accaagaagg gcgtggtcgt gatgaacacc cccggcggca
     4081 acaccgtgac gaccgccgag cacgccgtat cgatgatgat ggccctctcc cgccagatcc
     4141 cgcaggcgac cgcatcgatg aaggcgggga agtgggaaaa gaacaagttc atgggtaccg
     4201 agatcaccga caaggttctc ggcgttgtcg gcctcggagc catcgggaaa gtcgttgcgg
     4261 accgcgcgct gggggtcaag atggtcgtgg tcgccttcga tccgtatgta tcgaaggagg
     4321 atgctgcgct cctcggcgtg gagacggcga ccctcgacga gctgtacgcc cgtgccgact
     4381 acatcacgta ccacacgccg ctcacgcagg aaacgaaggg gatggtgaac gccgcctcga
     4441 tcgcgaagat gaaggacggc gttcggatca tcaactgcgc ccgcggggcg ctcgtgaacg
     4501 aagcggacct gctggcggct ctccagtccg gaaaggtcgc cggagcggcg ctcgacgtct
     4561 tcgcgacgga gcccccccct cccgacatgc cgctgctggc gcatcccaac gtgatcctca
     4621 cgccgcacct gggcgccgca acgacggagg cccaggaaaa ggtggcggtg ctgatcgcgg
     4681 agcagatctg cgatttcctg aaaaagggga cgatccgcaa ctcggtgaac ttcccgtccg
     4741 tcgccggcga acttctgccg gtgctgaagc cgttcctggc cctcgccgag cggctcggcg
     4801 cgttccacgg gcagctgctc cggaatccgg tccgggagct caaggtcgac tacttcggcg
     4861 aggtggggaa actctccacg gccccggtca ccatctcggt gctgaagggc ctcctgcagt
     4921 accagaccga ggaggtgaac ctcgtcaacg cccggatggt cgccgaggaa cgcggcatca
     4981 aggtgagcga gtcgaagacc cccaagtcgg agccgtacgc gagcctcctc aaggtcaccg
     5041 tggtgaccaa ggagggggag tcgtccgtgg ccggcaccgt gttcggaggg acgccgagga
     5101 tcgtccagat cgacgccttc gccatcgtgg cggagctctc cggcggcatc ctgatgctgc
     5161 ggaaccagga cgtcccgggc gtggtcggcc ggatcggcac cttcctcggg gagaagggga
     5221 tcaacatcgc cggcttgcgg ctgggcagga ccgaggtcgg cggaaccgcc gtttcgctca
     5281 tcaacgtgga caacgccgtc cccgagaacg tccttgcgca gcttcgaaag cttccgaaca
     5341 tcatggacgc ccaatacctt attttctgac gaaagaaggc gaactccttt gaaaagcgtc
     5401 atcgtgctcg gcgcccagtg gggcgatgag ggcaagggaa agatcgtcga catctattcc
     5461 gagttcgccg acacgatcgt gcgtcccacg ggcgggaaca acgccgggca caccctcgtg
     5521 gtcaagggcg agaagttcgt cttccacctc atcccctccg gcatccttca cccggggaag
     5581 aagtgcgtca tcgccaacgg ggtggtgatc gacccgaagg tgctcctcat ggagatcgac
     5641 cgcctgaagg agcgcggcta cctgaaggac gacgcgcagt tgaagatcgg gctgctggcg
     5701 aacatcatcc tcccgtacca catccggctc gacaaggcgc gggaagagaa gctcgggaac
     5761 aggaagatcg ggaccacggc gcgggggatc gggccgtgct acgaggacaa gatcgcccgc
     5821 atcggcatcc gggcgtgcga cctcgtccg
//
LOCUS       DASVJL010000367         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_717381, whole genome shotgun sequence.
ACCESSION   DASVJL010000367 DASVJL010000000
VERSION     DASVJL010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717381"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VF357_07765"
     CDS             complement(<1..1284)
                     /locus_tag="VF357_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7524083.1"
                     /translation="MTAWSVFIVDDEESIREGIRIALEPGCRVRVFLDAESAVEAVKE
                     DPPDLVLMDIGLPGMSGVEGIRAIKALRPETLIVVITAYEDVQTVVDAMKGGAFDYLV
                     KPLHMETLETTIGNALETVRLHKEVRELQERFLREKVPLFVGESHAIREVMEFVESVA
                     KSPDTPVLILGPTGTGKELVAAAIHYRSPNFRGPLVSVNCAAIPKELLESELFGYEKG
                     AFTGASLAGKKGLVEEASGGTLFLDEVADLSPEAQAKLLRFLEDGEFYRVGGTRRLRA
                     SARVVSATNKELETLVGKGLFREDLYYRLAVVRVAVPTLSDRRDDILPIARHFLAEFS
                     RKFGRAVSGFSPEAEGALLACPWEGNIRELRNVVERAILSGKGTEVAAADLRLTPGGA
                     GHRIPPSAGPSGPPLTSEGIDLPGVQESIERRYIEE"
     gene            complement(1281..>2718)
                     /locus_tag="VF357_07770"
     CDS             complement(1281..>2718)
                     /locus_tag="VF357_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7524084.1"
                     /translation="VSIFFSIAAELLDDLRPDTFMYGSLLGHYLNVVSFYLVYKAIVA
                     TGLIRPYDLLFRNLKRSEEEIHIARDELEARVTERTEELRVVNVRLERELSERIRMEE
                     ERGRTSHLLWESEDRFRSLVENELVGTMIVQEGRIVFRNPEQERLMGLIPDGFDFRRL
                     GPPHPEDVPKFERLCGTVREGEPFRQGMELRFSVPDAETGRRVFRWVQCRTVPIDFQG
                     KTAVLINMVDITRMKDLEQIVMAREKLASLGQLAAGIAHEIRNPLSGININISTMDLL
                     CRRAEGLDPEEKGKIHAVVAQAKAASDKIGAVVKRIMEYSKPIPPRMERVDVVRVVRE
                     TLALSATSIRKRDVGLRQELPPEPLYCRADATLLEQVVLNLITNALQALETVDREKTL
                     RVAVAQEGQQVVIRVVDNGPGVAGELLGKIFDSFYTTRKDGHGIGLSFSHRVISEHNG
                     RLSAGAAEGGGAAFRIELPLWRGDRRTA"
BASE COUNT          449 a          946 c          843 g          480 t
ORIGIN      
        1 ttcttcgata taccgcctct cgatcgactc ctgcaccccg ggcaggtcga tcccctccga
       61 tgtgaggggg gggcccgagg ggccggcgga aggcggtatg cggtggccgg cgcccccggg
      121 tgtcagccgc aaatcggcgg cggcgacttc cgttcccttt cccgagagga tcgcacgctc
      181 cacgacgttg cgaagttcac ggatgttccc ctcccatggg cacgcgagga gggccccttc
      241 cgcttccggg gaaaacccgc tcacggcgcg tccgaacttc cgcgaaaact ccgcgaggaa
      301 gtggcgcgcg atcggaagga tgtcgtcgcg ccggtcggaa agggtcggca ccgccacccg
      361 tacgaccgcg agccggtagt agagatcctc gcggaagagc cccttcccga cgagggtttc
      421 cagctccttg ttcgtcgcgg atacgaccct cgcggacgcg cgcaggcggc gcgtcccgcc
      481 gacgcgatag aactccccgt cctcgaggaa ccgcagcagt ttcgcctggg cttctggact
      541 caggtccgcc acctcgtcga ggaagagcgt accgccggat gcctcctcca ccagtccctt
      601 cttcccggcg aggctcgcgc cggtgaaggc gcccttctcg tacccgaaca gctcgctttc
      661 gagaagttcc ttcgggatgg ccgcgcagtt gacgctcacg aggggcccgc ggaagttcgg
      721 gctccggtag tggatcgcgg ccgcgacgag ttccttcccc gttcccgtcg ggccgaggat
      781 gagcacgggc gtatcggggc ttttcgcgac cgactccacg aactccatga cttcgcggat
      841 cgcgtggctt tccccgacga acagcggcac cttctcccga agaaaccgct cctgcaactc
      901 ccgaacctcc ttgtggaggc gaaccgtctc cagcgcgttc ccgatggtcg tctccagggt
      961 ctccatgtgc aacggcttca ccaggtaatc gaacgccccg cccttcatcg cgtcgacgac
     1021 cgtctggaca tcctcgtacg ctgtgatcac gacgatcagc gtttcgggac gaagggcctt
     1081 gatcgcgcgg atcccttcga cgccgctcat cccgggaagc ccgatgtcca tgagaacgag
     1141 gtccggcgga tcctccttga cggcctcgac ggcggactcc gcgtcgagga acactcgcac
     1201 acggcagccc ggctcgaggg caatccggat cccctcccgg atgctctcct cgtcgtccac
     1261 gatgaaaacg ctccaggcgg tcatgcggtc ctcctgtccc cccgccacag ggggagctcg
     1321 atgcggaacg ccgcacctcc cccttcggcg gcgccggcgg acagccggcc gttgtgttcg
     1381 gagatgacgc ggtggctgaa cgagagaccg atcccgtggc cgtccttccg cgttgtgtag
     1441 aaagagtcga agatcttgcc gaggagctcc ccggccacgc ccgggccgtt gtcgacgaca
     1501 cggatcacca cctgctgtcc ctcctgcgcc acggccaccc tgagggtctt ctcccgatcg
     1561 acggtctcca gcgcctggag tgcgttggtg atgaggttaa ggaccacctg ctccaggagc
     1621 gtggcgtccg cccggcagta cagcggctcc ggcggaagct cctgccggag cccgacgtcc
     1681 cgcttgcgga tgctggtcgc cgaaagggcg agggtctcgc ggacgacccg gaccacgtcg
     1741 acccgctcca tccggggagg gatcggcttg gagtactcca tgatccgttt gacgaccgcg
     1801 ccgatcttgt cggaagcagc cttcgcctgg gcgacgaccg cgtggatctt ccccttctct
     1861 tccgggtcca acccttccgc ccggcggcag agcaggtcca ttgtggagat gttgatgttg
     1921 atcccggaga gggggttccg gatctcgtgg gcgatcccgg cggccagctg gccgagggag
     1981 gcgagctttt cccgtgccat cacgatctgc tcgaggtctt tcatgcgcgt gatgtcgacc
     2041 atgttgatga ggacagcggt cttcccctgg aagtcgatcg gaacggtccg gcattgcacc
     2101 cagcgaaaca ccctccgccc cgtctcggcg tcgggcacgg agaaccggag ctccatcccc
     2161 tgccggaacg gctccccctc ccgcaccgtt ccgcaaagcc gctcgaactt cgggacgtcc
     2221 tccgggtggg gaggcccaag ccgtcgaaaa tcgaacccat ccgggatcag acccatcaga
     2281 cgttcctgtt ccgggttcct gaagacgatg cggccctcct ggacgatcat ggtgccgacg
     2341 agctcgttct ccaccaagga ccggaaccgg tcctcgctct cccagagcag gtggctcgtc
     2401 cgcccccgct cctcctccat ccggatccgc tcggagagct cccgctcgag ccggacgttc
     2461 acaacccgaa gctcttccgt ccgctcggtc acgcgggcct ccagctcatc ccgcgcaatg
     2521 tggatctcct cttcgctcct tttgagattc cggaacagca gatcataggg acggatgagg
     2581 cctgtcgcga cgatcgcctt gtagaccagg tagaacgaga cgacattcag gtaatggccg
     2641 aggaggctgc catacatgaa tgtgtccggg cggagatcgt ccaacaactc ggcggcgatc
     2701 gagaagaaaa tggagacg
//
LOCUS       DASVJL010000368         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1470204, whole genome shotgun sequence.
ACCESSION   DASVJL010000368 DASVJL010000000
VERSION     DASVJL010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470204"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1112
                     /gene="glk"
                     /locus_tag="VF357_07775"
     CDS             108..1112
                     /gene="glk"
                     /locus_tag="VF357_07775"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX7524085.1"
                     /translation="MILAGDVGGTKTSLALYRREARGLLRNRMATYRSREYASLDPIL
                     RDFLGGGATVERACIGVAGPVEGGRCRLTNLDWEVDEASLHGILGVREAYLVNDLQAT
                     ASSLPFLRESDRATIREGAANPRGNMAVLAAGTGLGEGFLVDTGPGYIPFPSEGGHVD
                     FGPRDDREIRLHAFLRAKYGHVSVERILSGPGLHDIYRFLLEEERMEEEPIIAAEVVG
                     REPQRAIVRHGLAGGPGPCAETLRIFCSLYGAEAGNLALQYLATGGVYLGGGIAPAIL
                     PALRRGEFQSAFLDKGRMRTLLSSVPVIVILDPAAPLLGAAAYAAAGGVLAHPPGSRT
                     "
     gene            1159..>3034
                     /locus_tag="VF357_07780"
     CDS             1159..>3034
                     /locus_tag="VF357_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7524086.1"
                     /translation="MGNAIPFPVGSPKGVRFPSRDVPISAVPRIARICALGLAFDELL
                     DEVCREILALSGADGGCLFLSSREERSETFSPAAESGTLPDVSGAYRPGPALDRLLDR
                     MRTEGRIQADDLSSLPASDPLKRLLDPFPVRSALLIPLRFGTGLLGFVALHVTGEPKP
                     WGSEALSAMDMVAAILSAALERRRVEDRLRASEARYRFLAEHSPDLITLHDATGRILY
                     ASPASLPMLGVRPELMNGSPVEGFLHPDDAGNVVAEIRRTAGGEKPDVPLPYRMRAAD
                     GRFVDVESSSTAFPEGPEGDRRVLRVTRDISGRRKMEARLGEIQTLSTIGMLAGGVAH
                     EFNNVLAAIQGAVELLSMHAVGNPQTLSYLDVIRRMANRATELTRQLLAYSRQGKYEP
                     ASIPLARVLSETLPAIRTSLPPGVELSTECDDALPPVHVDIAQMKQVVMGLCLNAAEA
                     MAGGGRLAVRTYSEPGDGRIVLEVSDTGPGIDAKAMPRIFDPFFTTKGVGRGMGLAAI
                     RGIVENHGGEIRVVSLEGKGTTSTVLLPVSAVPAAEEPPVTEGQSAGAGRVLLADDEE
                     DVRGVVHAMLETLGYEVIEARDGLEAVGIFRRRAAAIDLVILDLVMPRLTGEAALGQI
                     R"
BASE COUNT          420 a         1020 c         1108 g          486 t
ORIGIN      
        1 gaggaggttt gcgcccgcct ggaggaggag ggggtgaaga gcttcctcga ttcgtaccgg
       61 aagctcctcg ccgcggtcgg ggggcggctg aacagcgcct cgggcggatg atcctcgcgg
      121 gggacgtcgg cgggacgaag accagcctcg cgctctaccg gcgggaggcg cggggccttc
      181 tccgcaatcg gatggccacc taccggagcc gggagtacgc gagtctcgat ccgatcctgc
      241 gcgacttcct gggcggggga gccaccgtcg agcgtgcgtg catcggagtg gccggacccg
      301 tcgagggcgg ccgctgccgg ctgacgaatc tcgactggga ggtggacgag gcgtccctgc
      361 acgggattct gggggtgcgc gaagcgtacc tcgtcaacga cctccaggcg accgcttcct
      421 ccctcccgtt cctgcgggag tctgaccggg cgacgatccg ggagggggcg gccaacccgc
      481 gagggaacat ggcggtcctt gcggcgggaa cggggttggg ggaggggttt ctcgtcgaca
      541 ccggtcccgg gtacatcccg ttcccctcgg aagggggcca cgtcgatttc gggccgcgag
      601 acgaccggga gatccggctc cacgctttcc tgcgggccaa gtacggccac gtgagcgtgg
      661 agcgcatcct gtcgggcccc gggctgcacg acatctaccg gtttctcctc gaagaggaac
      721 ggatggagga ggaaccgatc atcgcggcgg aggtcgtcgg cagggaaccc cagcgggcca
      781 tcgtgcggca cgggcttgcc ggggggccgg ggccgtgcgc cgaaacgctt cgcatcttct
      841 gctccctcta cggggcggag gcggggaacc tggccctgca atacctcgcg accgggggag
      901 tgtacctcgg cggtgggatc gcgccggcga tccttcccgc gctccggcgg ggggaattcc
      961 agtcggcctt tctcgacaag gggcggatgc ggacccttct ctccagcgtc ccggtgatcg
     1021 tcattctcga ccccgcggcc ccgttgctcg gcgctgcggc gtacgcagcg gcgggcgggg
     1081 tgctcgcgca tccgcccggt tcccgcacgt gacccgggcc gcgatataat tccgttgtcg
     1141 gttcccgtgg aggtgtcaat gggcaatgcg atcccgtttc ccgtaggctc accgaagggg
     1201 gtcaggttcc cttcgcggga cgtcccgatc tctgccgtgc cgcggatcgc ccggatctgc
     1261 gccctgggac tggccttcga cgaactcctg gacgaggtgt gccgggagat cctcgccttg
     1321 agcggcgccg acggcggttg cctcttcctg agttcccggg aagagcgttc cgaaacgttt
     1381 tcgccggcgg cggagtcggg aacgcttccc gacgtcagcg gggcgtatcg tcccggcccg
     1441 gccctcgatc gcctgctcga ccggatgcgg acggagggga ggatccaggc ggacgatctc
     1501 tcctccctgc cggcgtcgga tccgttgaag cggctcctcg atccgttccc cgtccgttcg
     1561 gcgctgctca tccccctccg gttcggaacc ggcctgctcg gattcgtggc cctgcacgtc
     1621 acgggcgagc cgaagccgtg ggggagcgag gcgctatccg cgatggacat ggtggcggcg
     1681 atcctctccg cggcgctgga acgccgccgt gtcgaagacc gcttgcgtgc gtccgaagcg
     1741 cggtaccggt tcctcgcgga gcactccccc gacctcatca cccttcacga cgcgacgggg
     1801 cgcatcctct acgcgagccc ggcgtccctt ccgatgctgg gggtccgccc ggagctgatg
     1861 aacggctcgc ccgtcgaggg gttcctccac cccgacgacg ccgggaacgt cgtcgcggag
     1921 atccgtcgca cggccggcgg ggagaagccg gacgtgcccc tcccgtaccg gatgcgggcc
     1981 gccgacgggc gattcgtgga cgtggagtcg tcgtcgaccg ccttcccgga aggtccggaa
     2041 ggggaccggc gcgtcctccg cgtgacccgc gacatctccg gtcgaagaaa aatggaggcc
     2101 cgcctcggcg agatccagac gctctccacg atcgggatgc tcgccggcgg ggtcgcccac
     2161 gagttcaaca acgtcctcgc cgcgatccag ggcgccgtcg agcttctttc gatgcacgcc
     2221 gtggggaacc cccagaccct ctcgtacctc gacgtgatcc ggcggatggc gaaccgcgcc
     2281 acggagttga cccggcagct cctggcgtac tcccgccagg ggaagtacga gcccgcttcg
     2341 atcccgttgg cgcgcgtgct gtcggagacc ctcccggcga tacggacctc cctccctccc
     2401 ggggtcgagc tctctacgga atgcgacgac gcgcttccgc cggtccatgt ggatatcgcg
     2461 cagatgaagc aggtcgtgat ggggctttgc ctgaacgccg cggaggcgat ggcgggcggc
     2521 ggccggcttg cagtccgcac gtattccgag cccggagacg gtcgcatcgt cctcgaggtg
     2581 tcggacacgg ggcccgggat cgacgcgaag gcgatgcccc gcatcttcga tccgttcttc
     2641 accacgaagg gcgtcggccg ggggatgggg ctggcggcga tccgggggat cgtcgaaaac
     2701 cacggcggcg agatccgggt cgtctccctg gaggggaagg ggacgacgag cacggtcctt
     2761 cttccggtga gcgccgtccc cgcggcggag gagccgcccg tcacggaggg gcagtcggcg
     2821 ggcgccggcc gggtgttgct ggccgacgac gaggaggacg tacgcggcgt ggtccacgcg
     2881 atgctcgaaa ccctcgggta cgaggttatc gaggcccggg atggactcga ggccgtggga
     2941 atcttccggc gccgcgccgc ggcgatcgat cttgtgatcc tcgacctcgt gatgccccgc
     3001 ctgacggggg aggccgccct ggggcagatt cgcc
//
LOCUS       DASVJL010000369         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_907423, whole genome shotgun sequence.
ACCESSION   DASVJL010000369 DASVJL010000000
VERSION     DASVJL010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907423"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VF357_07785"
     CDS             <1..288
                     /locus_tag="VF357_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524087.1"
                     /translation="SNLIIAVFAVVVVGGMGSIMGSIVTGIGLGLIEGIVKSGMIANL
                     LSSMFDSVPVAGSIVYPIKLLFSPEASAVTIFVIMAIVLLVRPAGLFGRER"
     gene            292..1215
                     /locus_tag="VF357_07790"
     CDS             292..1215
                     /locus_tag="VF357_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7524088.1"
                     /translation="MPLRKMNAVAWGLLVVAGIAAPFLVYPVFAMKILCLALFACAFN
                     LLLGYTGLLSFGHAAFLGTAGYVTGHAVKVLGFQPEIGILAGTAAAAALGYVIGSLAI
                     RRSGIYFAMITLALAQMVYFMSLQMPFTGGEDGLQGVPRGKLFGMIDLMQPLYMYYFV
                     FAIFLPGFWVIYRTIHSPFGQVLKAIRENEPRAISLGYDVEKYKLTAFVLSAALSGLA
                     GATKTLVFQLASLTDVHWHTSGEVVLMTLLGGMGTVFGPVIGAIVVVSLQNYGANIGE
                     WVMVITGTIFVVCVLAFRRGIVGEIAVLFKK"
     gene            complement(1247..2860)
                     /gene="pckA"
                     /locus_tag="VF357_07795"
     CDS             complement(1247..2860)
                     /gene="pckA"
                     /locus_tag="VF357_07795"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR00224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX7524089.1"
                     /translation="MCGHLNGNRADNLSFHGIRKAEAVWWNLSRSALFEQSLRRREGH
                     LAASGPLVVRTGEYTGRSPNDRFFVREPGSEGSIQWGTTNRPFDADKYEALRARLLAY
                     LEGRELFVQDCHVGADEAHRLPIRVITEMAWHALFARNMFLPVTDREALPGHVPGFTV
                     ISAPGFHSRPEMDGTRSEVFILIHFGRKEVLIGGTLYAGEIKKSIFTVMNYLLPAAGV
                     LPMHCAASYGRDDQDVAVFFGLSGTGKTTLSADPERTLVGDDEHGWSDRGVFNFEGGC
                     YAKVINLSPGMEPEIHRTTGMFGTILENVGMDIEERRIDLNDASLTENTRASYPLSSI
                     PRVAPRGAVGHPKHIVMLTADAFGVLPPISEVTTDQAMYHFLSGYTAKVAGTERGVVE
                     PQATFSTCFGGPFMPLDPSVYAKLLGEKIAVHDVKVWLVNTGWTGGPYGTGHRMAIGH
                     TKAMLRAALSGKLDGVEMQVDPIFGLRVPRSCPNVPSEVLDPRSTWKDRAAYDRTARK
                     LAGMFQENFAQYADQVTPEVRAAGFRKTG"
BASE COUNT          471 a          955 c          976 g          593 t
ORIGIN      
        1 tcgaatctca tcatcgccgt gttcgcggtc gtggtggtcg gcggcatggg ctcgatcatg
       61 ggttccatcg tgaccggcat cgggctcggc ctgatcgagg gaatcgtcaa gtccgggatg
      121 atcgccaacc tgctcagctc gatgttcgat tcggttcccg tggcgggatc catcgtctat
      181 ccgatcaagc tgctgttttc ccccgaggcg tcggcggtga ccatcttcgt gatcatggcc
      241 atcgttttgc tggtgcggcc ggcgggcctg ttcgggaggg agcggtaagg catgcccctg
      301 agaaaaatga atgccgtggc ttggggtctg ctcgtcgtgg ccggcatcgc ggctcccttc
      361 ctcgtgtacc cggtgttcgc gatgaagatc ttgtgtctcg cgctgttcgc gtgcgcgttc
      421 aacctgctcc tcggctacac cggcctgctc tccttcgggc acgcggcgtt ccttggcacc
      481 gccgggtatg tcacgggcca tgccgtcaag gtcctcgggt tccagcccga gatcggcatc
      541 ctggcaggca ccgcggcggc cgcggcgctc gggtacgtca tcggcagcct ggcgattcgg
      601 cgctcgggga tctacttcgc gatgatcacc ttggccctgg cgcagatggt ttacttcatg
      661 tcgctgcaga tgcccttcac gggaggggag gacgggctgc aaggggtgcc gcgcggcaag
      721 cttttcggca tgatcgacct gatgcaacct ctgtacatgt actacttcgt gttcgcgatc
      781 ttcctgccgg gattctgggt catctaccgg acgatccatt caccgttcgg ccaggtgctc
      841 aaggcgatcc gggaaaacga gccgagggcg atttcccttg gatatgacgt cgaaaaatat
      901 aaattgaccg catttgtgct ctcggcggcc ctttcgggcc tggcgggggc gaccaagacc
      961 ctggtgttcc aactggcgtc tctcaccgat gtgcactggc acacatcggg ggaagtggtg
     1021 ctgatgaccc tgttgggcgg gatggggacg gtattcgggc ccgtgatcgg cgcaatcgtc
     1081 gtggtcagcc tgcagaacta tggagcgaat atcggcgaat gggtgatggt gatcaccggt
     1141 acgatttttg tcgtctgcgt gttggcattc cgccgcggca tcgtgggcga gatcgcggta
     1201 ttgttcaaaa aatgacctcc gccgccggag gaaggacggg aacgggtcag cccgttttgc
     1261 ggaaacccgc ggcccggacc tcgggcgtca cctgatcggc gtactgggcg aagttctcct
     1321 ggaacatccc ggccagcttc cgcgccgtcc ggtcatacgc cgcgcgatcc ttccacgtcg
     1381 acctcgggtc caggacctcg gacggcacgt tcgggcagct cctcggcacg cgcaatccga
     1441 agatcggatc cacctgcatc tcgacgccgt ccagcttccc ggaaagcgcg gcacggagca
     1501 tcgccttggt gtggccgatc gccatccggt gtcccgtgcc gtacggaccg ccggtccatc
     1561 ccgtgttgac cagccagacc ttgacgtcgt gtacggcgat cttctccccg aggagcttcg
     1621 catagacgga agggtctaat ggcatgaacg ggcctccgaa acaggtgctg aacgtcgcct
     1681 gcggctccac gaccccccgt tcggtgccgg ccaccttggc cgtgtatccg gagaggaagt
     1741 ggtacatcgc ctggtcggtc gtcacctccg agatcggcgg aagcaccccg aaggcgtccg
     1801 ccgtcagcat cacgatgtgc ttcgggtggc cgacggcgcc gcggggagcg acccggggga
     1861 tggaggagag cgggtaggag gcgcgggtgt tttccgtgag ggatgcgtca ttcaggtcga
     1921 tccgccgctc ctcgatatcc atccccacgt tttcgaggat cgtcccgaac atcccggtcg
     1981 tccggtgaat ctccggctcc atcccggggg agaggttgat caccttggcg tagcagcctc
     2041 cctcgaagtt gaagacgccg cggtcgctcc agccgtgctc gtcgtccccc accagcgtcc
     2101 gctccgggtc ggcggagaga gttgtcttcc ccgttcccga gagaccgaag aagaccgcca
     2161 catcctgatc gtcgcgccca tagcttgccg cacagtgcat cggcaggacg cccgctgcgg
     2221 ggagcaggta gttcatcacg gtgaagatcg acttcttgat ctctcccgcg tagagggtgc
     2281 cgccgatcag cacttccttg cgcccgaagt ggatcaggat aaagacctcg ctccgcgtcc
     2341 cgtccatctc ggggcgggag tggaagccgg gtgcgctgat gaccgtgaac cccgggacgt
     2401 ggcccggaag cgcctcccgg tctgtcacgg ggaggaacat gttccgcgcg aacagggcgt
     2461 gccacgccat ctcggtgatg acccggatcg gaagccggtg cgcctcgtcc gcgcccacgt
     2521 ggcaatcctg gacgaagagc tccctccctt cgaggtaggc gagcagccgg gcccgaagcg
     2581 cctcgtattt gtcggcgtcg aagggccggt tcgtcgttcc ccattggatc gacccctcgc
     2641 ttccgggctc ccgtacgaag aagcggtcgt tgggggaccg gcccgtgtac tcgccggtcc
     2701 gcacgacgag gggaccggac gccgccaggt gcccttcgcg acgccgcagc gactgctcga
     2761 acaacgccga acgcgagagg ttccaccaga cggcctcggc cttccgtatt ccgtggaagg
     2821 agagattgtc ggcccggttc ccgttcaaat gcccacacaa ggcgagcctc ctcgttgccg
     2881 tcgatcgacg cgaatcttac cggtttattt taccactccg cgcggcgatg ctcggcgtgg
     2941 cggggggccg gcttgctcgc cccgaacccc ggagtccact tcgattcgcg gcgcg
//
LOCUS       DASVJL010000370         7108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_812368, whole genome shotgun sequence.
ACCESSION   DASVJL010000370 DASVJL010000000
VERSION     DASVJL010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812368"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VF357_07800"
     CDS             complement(<1..291)
                     /locus_tag="VF357_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524090.1"
                     /translation="MPVSKTGSLSRRLRVLPFLAILALALSASGCRSPFFHPTREHVP
                     VPGLDNVVRQDVRFASEDGVPLHGWLLSPRDRVSRGTILFLHGNAKNISTHVQ"
     gene            354..1910
                     /locus_tag="VF357_07805"
     CDS             354..1910
                     /locus_tag="VF357_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7524091.1"
                     /translation="MLLQRHGRALLLLFLVSGCAQKLPVDEGRLVIALPGAPVNVDPR
                     VATDAYGEQILQMTHASLVRRDTAGEPIPDLAERWESPDPLTYIFHLRPGLRFHGGRT
                     LTSADVRYTFTWILDAANRSPHRGLYRHIAAIETPDPRTVVFRLSEPFAPFLSTMVRG
                     IVPAGSPARGYAPPVGAGPYKIDDLSPDGEAVLSAYDGYYGGPPAIRAVTVKFVPDSN
                     VRFLELKMGSVNFTLNGVDPDLLPAAERDRRLVVEEGPGGNVTYLGFNLRDRALSDPK
                     VRRAIALAIDRETIVRTIWKGRADLVSSILPPGSWAHDPDLPAVPFDPARARRLLDEA
                     GYSDPDGEGPLPRLRLTYKTSQNEVRRRVATVIQEQLRQVGIAVEIQSLEWGSFFSDI
                     KKGNFQIYSLTWVGIADPDIFHHAFHSRSAPPDGANRGGYSDVEVDRLTEAARREPSR
                     EKRREMYRRVQRILARDLPVFPLWAGRNLLVRDRRLAGFQLTPDESYASVRAMRIVPE
                     SSTPPTAGRR"
     gene            1907..2827
                     /locus_tag="VF357_07810"
     CDS             1907..2827
                     /locus_tag="VF357_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7524092.1"
                     /translation="MRGYLLGRLLRTVPVVFGVVTVVFLLIHLLPGDPVEIMLGESAV
                     PAQKEELRRELRLDRPILSQYASYLAGIPRGDLGVSFRSREPVLGEIARRIPATVLLA
                     ATSITVAVFVALPLGVLAAMRRGKAADYLSGFAAMLSLSIPNFLLGPLLVLLFSVQLG
                     LLPVSGYGAGRPLILPAITLGTGMAALLTRMVRATLLEELRKEYVTAAIARGLPYRSA
                     VARHALRNALVPVVTALGLSFGAVLAGSVITETIFSWPGIGRLLIQAIDARDYPLVQG
                     CVLVIALSTVAVNLATDLLCARLDPRIRYE"
     gene            2820..3674
                     /locus_tag="VF357_07815"
     CDS             2820..3674
                     /locus_tag="VF357_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7524093.1"
                     /translation="MNEESGFLALIGRTARHRGAAVGMVLLVLFLLAAVFAPVIAPRD
                     PLAQSLDEGLSGPSSSHWLGQDKFGRDVLSRLIHGARLSLAVGLGTVGISLLIGLAVG
                     SLAGYLGGAADRIFTGACDVLLAFPGILLAIGIAAVRGPSFGNVLFALSVLGWVGYAR
                     VIRAQVLSVKTREFVESARAVGSSPARLLLRHILPNAISPILVEATFGIARAIVAEAG
                     LSFLGLGVAPPMPSWGSMIGEGRHFLFIAPHLVTAPGAAILLTVMAFNFLGDGLRDAL
                     DVTTTTTP"
     gene            3690..4826
                     /gene="nagZ"
                     /locus_tag="VF357_07820"
     CDS             3690..4826
                     /gene="nagZ"
                     /locus_tag="VF357_07820"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX7524094.1"
                     /translation="MKHGDRIASPLWVGFDGKSLPASLSRWLAGGQVGGVVLYSRNIE
                     SPAQVRALCHGIRSAAGRGNPLPLIAVDQEGGRVARFKDPPFTWFPPARACSLFCCRN
                     EAVAETVGAATAAELRAVGIDVNFAPVLDVDSNPANPVIGDRAFSEDPHTAAALGIAF
                     AKGSLSRGILPVGKHFPGHGDTSADSHKELPIVRAGRQTLLRRELLPFRRAARAGIPA
                     LMTAHVMYPSLDRALPATLSRKILHDLLRKRLRFRGAVISDALEMKAIADRYGIGEAA
                     VLAMTAGCDVVLVCRGENEQAETIDRLTREATDRPSFRRAVAAAAVRSGRLRAWAAAK
                     ERCRRAPRSLGAERHRLLSSLLREAWESTGRTSPDGKSGNIGEG"
     gene            complement(4909..6876)
                     /locus_tag="VF357_07825"
                     /pseudo
     CDS             complement(4909..6876)
                     /locus_tag="VF357_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
BASE COUNT         1027 a         2531 c         2341 g         1209 t
ORIGIN      
        1 ctggacgtgg gtgctgatgt ttttcgcgtt gccgtggagg aagagaatcg tcccgcggga
       61 aacccggtcg cgcggggaca ggagccagcc gtggagcggg acgccgtcct ccgacgcgaa
      121 ccggacatcc tgccgcacga cgttgtccag ccctggaacc ggaacgtgct cccgcgtcgg
      181 gtggaaaaag ggggagcggc accccgacgc cgagagggcg agcgcgagga tcgcaaggaa
      241 cgggaggacg cggaggcgtc gagagagaga ccctgttttg gacaccggca tcgggatccc
      301 attttcccac attcgacgtc ctccccgcca tccccgggtg ctaccattcc agcatgctcc
      361 tgcagcggca cggtcgggcc cttcttctcc tgttcctggt ttcgggatgc gcgcagaagc
      421 tgccggtcga cgaggggcgg ctggtgatcg cgctgccggg ggcgcccgtg aacgtcgacc
      481 cgcgggtggc gaccgacgcc tacggcgagc agatcctcca gatgacgcac gcctccctcg
      541 tccgccgcga caccgccggc gagcccatcc ccgacctcgc ggaacggtgg gagtcgcccg
      601 atcccctgac gtatatattc caccttcggc cggggttgcg gttccatggc gggcggacgc
      661 tcacctccgc cgacgtgcgg tacaccttca cgtggatcct cgacgccgcg aatcggtccc
      721 cccaccgggg gctgtatcgg cacattgccg caatcgagac ccccgacccg cggaccgtcg
      781 tcttccgtct ctccgaaccg ttcgccccgt tcctctccac gatggtgcgc ggcatcgtcc
      841 ccgccggaag tccggcccga ggttacgcgc cgcccgtcgg ggcgggccct tacaagatcg
      901 acgacctctc ccccgatggc gaggcggtcc tttcggcata cgacgggtat tacggcggcc
      961 cgcccgcgat ccgcgcggtg acggtcaagt tcgtccccga cagcaacgtg cgcttcctcg
     1021 agctgaagat ggggagcgta aacttcaccc tcaacggggt cgaccccgac ctcctcccgg
     1081 cggcggagcg ggaccgacgg ctcgtcgtcg aggaaggccc gggggggaac gtcacctatc
     1141 tcggcttcaa tctccgcgac cgggcgctgt ccgacccaaa ggtgcggcgc gcgatcgccc
     1201 tcgcgatcga ccgggagacg atcgtccgga cgatctggaa ggggcgcgcc gacctcgtct
     1261 cctcgatcct tccccccggg tcgtgggcgc acgatccgga tcttcccgcg gtgccgttcg
     1321 atccggcacg ggcgcgacga cttctcgacg aagccgggta ctcggatccg gacggcgagg
     1381 gtcctctccc ccgtctccga ttgacgtaca aaacgtcgca gaacgaggtg cgccggcgcg
     1441 tcgcgaccgt gatccaggag cagctccgcc aggtcggaat tgccgttgag atccaatctc
     1501 ttgagtgggg atccttcttc tccgacatca agaaagggaa cttccagatc tacagcctca
     1561 cctgggtagg gatcgcggac cccgacatct tccaccacgc cttccattca cggagcgctc
     1621 cccccgacgg cgcgaatcgc ggggggtact ccgacgtcga ggtcgaccgc ctgacggagg
     1681 cggcgaggcg cgaaccgtcc agggaaaaac gccgggagat gtatcggcga gtgcagcgga
     1741 tcctcgcgcg cgacctgccg gtgttcccgt tgtgggcggg ccggaacctg ctggtccggg
     1801 atcgcaggct cgcgggattc cagctcaccc cggacgaaag ctacgcatcg gtccgagcga
     1861 tgcggatcgt cccggaatcg tcgacgccgc cgaccgcggg ccggcgatga ggggatatct
     1921 cctcggccgc ctcctgcgaa ccgttcccgt cgtgttcggg gtggtgaccg tcgtctttct
     1981 gctgatccac cttcttccgg gggatcccgt cgagatcatg ctgggggaga gcgccgtccc
     2041 ggcgcagaag gaagagttgc gccgtgagct gcgtctcgac cggccgattc tttcccagta
     2101 cgcctcgtat ctcgcgggga tcccgcgcgg ggacctcggg gtgtcgttcc gcagccggga
     2161 gccggtcctc ggtgagatcg cccgccggat tcccgccacc gtccttctcg ccgcgacgtc
     2221 catcaccgtg gcggtcttcg tcgccctgcc gctcggggtg ctggcggcga tgcggcgagg
     2281 gaaggccgcg gactacctgt cggggttcgc ggcgatgctc tccctgtcca tcccgaactt
     2341 tctcctcgga ccgttgctcg tgctcctctt ctccgtgcag cttgggctcc tccccgtgtc
     2401 cgggtacggc gcgggtcgtc cgctcatact gcccgcgatt acgctcggga cggggatggc
     2461 cgcgctgctg acacggatgg tccgggcgac gctgctggag gagctccgga aggagtacgt
     2521 gacggccgcg atcgcgcgtg gtctgccgta ccggtccgcc gtcgcccgcc acgcgctgcg
     2581 caacgccctc gtccccgtcg tcacggcgct ggggctgtcg ttcggcgcgg tgctggcggg
     2641 gagcgtcatc acggagacga tcttctcctg gcccgggatc ggacgcctcc tgatccaggc
     2701 gatcgacgcg cgggactacc cgctggtcca ggggtgcgtc ctcgtcatcg ccctgtccac
     2761 cgtggccgtg aacctggcga cggatcttct gtgcgcccgc cttgacccga ggatccgcta
     2821 tgaatgagga atcggggttc ctcgcgctga tcggccgaac ggcccggcac cggggcgccg
     2881 ccgtcgggat ggttctcctt gtcctgttcc ttctcgccgc cgtcttcgcg ccggtgatcg
     2941 cgccgcgcga cccgcttgcc cagtcgctcg acgaggggtt gtccgggcct tcctcctccc
     3001 actggctggg gcaggacaag ttcgggcgcg acgtcctttc ccggctgatc cacggggccc
     3061 gactctccct cgctgtcggg ctgggaacgg tcggcatctc gctcctcatc gggctggccg
     3121 tcggctccct cgccggctac ctcggcggcg ccgccgaccg gattttcacc ggcgcctgcg
     3181 acgtcctgct ggcgttcccg gggatcctgc tcgccatcgg gatcgccgcc gtgcgcggtc
     3241 cctccttcgg aaacgtcctg ttcgcacttt cggtcctcgg gtgggtgggg tacgcccggg
     3301 tgatccgcgc acaggtgctc tccgtgaaaa cgagggagtt cgtcgagtcg gcccgggccg
     3361 tgggcagctc cccggcgcgg ctgctcctgc gccacatcct ccccaacgcg atctctccga
     3421 tcctcgtgga ggcgaccttc gggatcgccc gcgccatcgt cgcggaggcg ggactttctt
     3481 tcctgggtct gggcgtcgcg ccgccgatgc cgtcgtgggg gtcgatgatc ggcgaggggc
     3541 ggcacttcct gttcatcgcg ccgcacctcg tgaccgcccc cggggcggcg atccttctca
     3601 cggtgatggc gttcaatttc ctgggagacg ggctccgcga cgccctcgac gtgaccacaa
     3661 ccacaacccc atgataaagt ctccatgcca tgaagcatgg agaccgcatc gcctcccccc
     3721 tgtgggtcgg cttcgacggg aagtcgcttc ctgcctcgct gtcgcgatgg ctcgcaggcg
     3781 ggcaggtcgg cggcgtcgtc ctctactccc gcaatatcga gagccccgcc caggtacgcg
     3841 ctctgtgcca tgggatccga tccgccgccg gccgtgggaa tccactcccg ctgatcgccg
     3901 tcgaccagga gggaggccgc gtggcgcggt tcaaggaccc cccgtttacc tggtttcccc
     3961 cggcccgggc ctgctccctc ttctgttgcc ggaacgaggc cgtggcggaa accgtcggcg
     4021 cggcgaccgc cgccgagctc cgcgcggtgg ggatcgacgt gaacttcgcc ccggtcctcg
     4081 acgtggacag caacccggca aacccggtga tcggcgatcg cgccttctcc gaagacccgc
     4141 acaccgcggc cgctctcggg atcgcgttcg cgaagggttc gctctcccgt gggatccttc
     4201 cggtcgggaa acatttcccg gggcacggcg atacgtccgc cgactcccac aaagagctgc
     4261 cgatcgtccg ggcgggacgg cagacgctcc ttcgccgcga gctgcttccc ttccgccgcg
     4321 ccgcgcgcgc ggggatcccg gctctcatga ccgcgcacgt gatgtacccc tcgctcgacc
     4381 gcgccctgcc ggccaccctg tcccggaaga tccttcacga cctgttgcgg aaacggttgc
     4441 ggtttcgcgg ggcggtcatc tccgacgcgc tggagatgaa ggcgatcgcg gatcgctacg
     4501 ggatcgggga ggcggcggtc ctcgccatga cggccgggtg cgacgtcgtg ctcgtgtgcc
     4561 ggggagaaaa cgaacaggcg gagacgatcg accgcctgac ccgggaggct accgaccgcc
     4621 cgtcgttccg gcgggcggtg gcggcggcgg cggttcgatc ggggcggctt cgggcgtggg
     4681 cggcggcgaa agaacgttgt cggcgggccc cgcggtcgtt gggggcggaa cggcatcgac
     4741 tgctttcttc actcctgcgg gaggcttggg aaagtaccgg acgaacatct ccggacggta
     4801 aatccggtaa tattggagaa ggttgagaaa gaccctccgc aggtagaagc gcgtctcctt
     4861 gtaattgatc ccttcgagga acgccgccgg atcgccgttc gcctgtttcc accaccgttc
     4921 caccgccgtc tctcccgcgt tgtacgccgc aatcgcccgg aaatagtccc ccccgtatcc
     4981 gtgcaccagg cgtgacaggt acgacgcccc cagccggatg ttcacggacg gacgcagcag
     5041 ttccttcctg cctggcttcg gcatcttctc cttccgcgcc acctccgccg ccgtcctggg
     5101 catcaactgc atcaggccca cggcgccggc gggggacagg acgtccgatt ggaaacgcga
     5161 ctcctgacgg atgaccgcat ggaggacgag cgggtcgatc ccggagcgtc ggcgatcgca
     5221 atcggcgagg aactcggggg caagaggata ttgaatccga tccgtgagcc ccgggtgctc
     5281 cggatcgagg cggacgtttg ccgtctcgcg gatcccccca ttcaggtcac cggcaagata
     5341 ccggaagagg ccctgcgccc caccgtcccc cgtcccgatc gccttccgcg ccgtcgttcg
     5401 gtcgacacgc tcggcctcga ggacggcgta gtcgatcacc ccgaggaggg tcagccgttc
     5461 cgcccgccgg atcttctcgg catcggccgg cccccagtcc gcattccgga tacgcgccca
     5521 gagccgttcg cgctcctcgc cgcacgcctt cgtctcgccg ttggaggagg cgttgaggaa
     5581 ccggaacgtc tccgctcccc gatccgaaac cgcgaagagc gcgtagatcc ctgcgaacgc
     5641 gtctccggcc aggtccgcga agatcgacgc ggcctccgct tccctgccgg agtcgcgaag
     5701 cgcccgcgcc ttccagtacc gatgccgggc gcgctccacc gtcccgcctc cccccgtctc
     5761 cccgacatcg aacgccgcga tcgcgtcgcc gcgtcggcct gtccggtaga gagcgtaggc
     5821 gaaccggaag agtgcctcct gccggatcga ttcgtccctg gcacccctga gacgtccgta
     5881 cgattccgtc gcccccgcga gatccccctc atcctcggcg atccacgcag cgaggtaccg
     5941 cgcccggtcc gccgtccccg gaagcgcatc cccctcggcg atttcgagaa actccttgcg
     6001 cgcctctgcg aggcgtcccg ccttccattc gacccgtgcg cggagaaagc gggcctccga
     6061 ccggatcgcc ggcggggcgt ccgccagaac cttttccaga agcgcggcgg ccgtcgcggt
     6121 ctccccctgc ttccggtgga actcggcgta gtcgaggacc agggcgaaat attgttccga
     6181 agcggggaat cgtgcggccg cctcttcgaa gagcgccctc gcccgttccg cgtccccggc
     6241 ccgggcagcc gccttggcga acttcgcgta gaaccccggc gacaggtccc accccgcgaa
     6301 gaggccgtcg gcacgccact tccaaaggcg ctcgaaggag aggatcccgg cgtcccgcgc
     6361 cgagtaggcc gagaaattct ccagggacag cgccgccgct tccgccgtgg ggcccccgtc
     6421 gccggcgagg aaggcggcga catacccttc ctccgccttt cgccggctgc cgctcacctt
     6481 gccgcgggag agcccgaacg cttcgcggga cgaggcgacg tcgttgtcga gggcggccgc
     6541 gtaggcgaca tcgtggcgaa gatacgggat caggggggag agggggaact tctccgtgag
     6601 caaacgcaac gactctcccg cttcggtccg cttcccctcg cgtgcggcgg actcccctgc
     6661 gaagtacacc acgtaatcac caaggtcgta ttcaagggga ggaatcttcg cgaatatttc
     6721 ccgggcctcc tcgtaccgtt ccttctgcag gagggaccac ccctcccgga acgcggcgtc
     6781 gtgccccggc gcggaggagg aggcgatcgc gaaatgcggg cacaggacga ggaaggcgag
     6841 aatccacatg aggcgcagcc ggttcggcaa tggcatctcc gggcggcgac tgccgggagg
     6901 cagaccgtca tgttattatc gcggagtagt gcactggaac acaagggagt gtagcgtccc
     6961 gcgaatcgcc tgacgcatcg agagggtcga tgcgccgcgc caggaaggac tccttgaacg
     7021 tttccctgtc gacgcacctg ttcgccttcc atgacctgga cgaggcgatt tttccgctct
     7081 ttccccggta cggcttctcc ctcgcgga
//
LOCUS       DASVJL010000371         6870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1000511, whole genome shotgun sequence.
ACCESSION   DASVJL010000371 DASVJL010000000
VERSION     DASVJL010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000511"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VF357_07830"
     CDS             <1..244
                     /locus_tag="VF357_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524095.1"
                     /translation="REDRPFHPHITVGRTRGALPPAWGERFVQVLSGKGFGVVPVSSF
                     TLYESRLGPGGAVYTPLCDFRLEGSGKREIREEKEP"
     gene            241..1269
                     /gene="recA"
                     /locus_tag="VF357_07835"
     CDS             241..1269
                     /gene="recA"
                     /locus_tag="VF357_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX7524096.1"
                     /translation="MSQDAGRRKALDMAVAAIEKNYGKGAIMRLGSDAPVEIVPIIST
                     GAISLDAALGIGGIPRGRVTEIFGPESSGKTTLALHIIAEAQRTGGIAGFIDAEHALD
                     LTYARKLGISTEDLLISQPDTGEQALEIGGMLVRSGALDVLVVDSVAALVPKAEIEGE
                     MGDAHMGLQARLMSQALRKLTGSISKSRTAVVFINQIRQKIGVMFGNPETTTGGNALK
                     FYATVRLDIRRIAQIKKDDSVIGGRTRVKVVKNKLAPPFREAEFDILYGEGISREGDI
                     LDLGVELDVVEKSGAWYSVGGERIGQGRENARLFLKEHPEMAGDLAKKILAKRGIVAA
                     GEAATEGA"
     gene            1274..2401
                     /locus_tag="VF357_07840"
     CDS             1274..2401
                     /locus_tag="VF357_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX7524097.1"
                     /translation="MELNEILRAAAKHGASDVHLKVGLPPVFRINGKLVPLKVPDPLK
                     PGDLVAMTEIIFQEGQKERFELRHELDCAYGVPGLGRFRVNVFQQRGTIGIVMRLVPV
                     GVRTFEDLHLPKVLEKISLEERGLILCTGTTGCGKSTTLAAMVQYINTHRSCHVMTIE
                     DPIEFLLRDGKSIINQRELGVDSSSFADALKSSLRQDPDVILVGEMRDLETIETAIVA
                     AETGHLVLSTLHTMDAAETINRIVATFPPFQQKQIRLQLGSVLKAVVSQRLLPRADGQ
                     GRVPAAEILLNTARVREYIEDKDKTRKIREAIAQGFVSYGMQTFDQSLLTLLKEGLVT
                     LDEVLRQASNPDDFALRVRGVSSTSDLTWDDFEKGSPPKPS"
     gene            2484..2888
                     /locus_tag="VF357_07845"
     CDS             2484..2888
                     /locus_tag="VF357_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX7524098.1"
                     /translation="MAQRLARKGVAEGEILPVLSRLRELGLLEDAALCGRLARSYLED
                     RRYGPAKIAWKLASRRFSRDLVEEALREVASPGAVAEAAAIALRKKFRNGIPPGREGA
                     AKAYRFLAGRGFPPDACRAAVGRGRTELSQGD"
     gene            2892..5522
                     /gene="alaS"
                     /locus_tag="VF357_07850"
     CDS             2892..5522
                     /gene="alaS"
                     /locus_tag="VF357_07850"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX7524099.1"
                     /translation="MSITGAQLRSGFLDYFGKNGHKILPGASLVPANDPTLLFTNAGM
                     VQFKEYFLGQSTADWKRATTAQRCLRVSGKHNDLENVGYTARHHTLFEMLGNFSFGDY
                     FKKEAIFFGWDFLTREVGLNGTRMTVSVFREDDEAYDLWHKTIGIPASRIVRFDEKDN
                     FWSMGATGPCGPCSEILYDQGEGVGCGRPGCAVGCDCDRFLEIWNLVFMQFNQDESGK
                     RTPLPKPSIDTGMGLERLASVVQGVTSNYDTDLFRPILDGISRQCGVPIGKSPALDAA
                     MRVVADHARATAFLIADGIVPSNEGRGYVLRRIMRRALRHGKKLGFDGPFLHKVAGIV
                     VDEFQEAYAGLAQSASFIDTVAFQEERRFLETLDAGLRMVEEEFSRMSGDRKIFPGEV
                     AFKLYDTYGFPADLTADLCRERGVALDSAGFEKEMDKQRAQSRDSWKGGEVGAQDALA
                     GELSRAGISVDFVGYERMESDAHVVAVFRQGARVASAASGEEAELVTDVTPFYGESGG
                     QAGDTGDVQGDGFALRVEDTRRVSSDQVVHRVKVLSGTLREGQAARLRVDETSRRLTE
                     GNHTATHLLQAALRKLLGTHVKQAGSYVGPDKLRFDFSHFEALSPETLSAVEEEVNRV
                     VFSPRPVTWESLPYEQAVAAGAMAFFGEKYADIVRMVTVPEVSRELCGGTHVRNTAEI
                     GLFHILSEGAVAAGVRRIEAVTGLPAWRCLREEAGTLHGVARELKVAAPEVPERVRKL
                     SAQIKALEKALQEARRRSSRDRVGEILSGAKEVAGVRRVSAEVEAMDAAALRDLADAV
                     KGKLLSGILLLGAREGERCHLVAGVTSDLTGRFSASDIVRKAAALVGGGGGGRKDMAQ
                     AGGPRVDNLPEALASISTWE"
     gene            complement(5646..6362)
                     /locus_tag="VF357_07855"
     CDS             complement(5646..6362)
                     /locus_tag="VF357_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7524100.1"
                     /translation="MSRELAGLIRMGKNAFSEADCLRAERHLLEALEGGAKYPDIHYT
                     LGLIDHQRGNYRRAVERFGQAVSLNPDYTEALLSLSITLNDMGRYDEARDAYDRASEI
                     LSRKGGSPGENLFRGRIANLHKELGELYLALGQHDDAIREYRHALSVAPGYPDLRVRL
                     VTALREAGRMDEARNEVDAFLAETPENPEALIQKGILHYLAGEKGWARRAWEEAHYKQ
                     PLNKIVQVYLNTIDRENPPG"
     gene            complement(6359..>6870)
                     /gene="bamD"
                     /locus_tag="VF357_07860"
     CDS             complement(6359..>6870)
                     /gene="bamD"
                     /locus_tag="VF357_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX7524101.1"
                     /translation="QFGLASNRLANKQEIEAEVAFDDFLRLYPADPKIPDALFMKGNL
                     LSRQVLAPGRDQGKTREAIKAYTLFLEREPGTPRAREISGKILELRNRLALHEANVVT
                     SFLSRKRYDSAEARARWAVATYPDVPAATKLLTLLVQALEKQGKRDEAETFRKKLAEK
                     DTGHGEKKR"
BASE COUNT         1131 a         2180 c         2388 g         1171 t
ORIGIN      
        1 ccgggaggac cgtccgttcc acccgcacat cacggtcggg cgtacccgcg gggcgctccc
       61 tccagcgtgg ggagaacggt ttgtccaggt gctttccggg aagggattcg gcgtcgttcc
      121 cgtgtcgtcg ttcacgttgt atgaaagccg ccttgggccg gggggggcgg tctatacccc
      181 gctttgcgat ttccgcctcg aaggctccgg caagcgggaa atacgagagg agaaggaacc
      241 atgagccagg atgcagggcg ccgcaaggcg ctcgacatgg ccgttgcggc gatcgagaaa
      301 aattacggca agggagccat catgcggctg gggtcggacg cccccgtcga gatcgtcccc
      361 atcatctcca ccggcgcgat ctccctcgac gccgccctcg gcatcggcgg gatcccgcgg
      421 ggccgggtca cggagatctt cgggccggaa tcgtccggta agacaactct cgcgcttcac
      481 atcatcgccg aggcgcaacg cacggggggg atcgccgggt tcatcgacgc ggagcacgcc
      541 ctcgacctca catatgcgag gaagctgggg atctccacgg aagacctcct gatctcccag
      601 cccgacacgg gagagcaggc cctcgagatc ggggggatgc tcgttcgcag cggcgcgctc
      661 gacgtcctgg tggtcgactc cgtcgccgcg ctggttccga aggcggagat cgagggggag
      721 atgggcgacg cccacatggg actccaggcg cgcctcatgt cccaggcgct gcgcaagctc
      781 accgggtcga tcagcaagtc ccggacggcg gtcgtcttca tcaaccagat ccggcagaag
      841 atcggggtca tgttcggcaa cccagaaacc accaccgggg gcaacgcact caagttctac
      901 gccaccgttc ggctggacat ccgccggatc gcccagatca agaaggacga ctccgtgatc
      961 ggcggccgca cgcgggtcaa ggtggtaaaa aacaagctcg ccccgccgtt tcgcgaggcg
     1021 gagttcgaca tcctctacgg ggaagggatc tcccgcgagg gcgacatcct cgacctcggg
     1081 gtggagctgg acgtcgtcga gaagagcggc gcgtggtact ctgtcggcgg ggagcggatc
     1141 gggcaaggac gggagaacgc ccgcctgttc ctcaaggagc atcccgagat ggccggcgac
     1201 cttgcgaaga agatcctcgc gaagcgcggg atcgtggcgg ccggcgaggc ggcgacggaa
     1261 ggagcgtgat gtcttggaac tgaacgaaat cctccgggcg gcggcgaagc acggggcctc
     1321 ggacgtgcac ctcaaggtgg gacttccccc cgtcttccgg atcaacggga agctcgttcc
     1381 cctcaaggtc cccgatccgc tgaagcccgg ggatctggtg gcgatgacgg agatcatctt
     1441 ccaggagggc cagaaggagc ggtttgaact gcgccacgag ctcgactgcg cctacggcgt
     1501 cccggggctg ggccggttcc gcgtcaacgt gttccagcag cgggggacga tcggcatcgt
     1561 gatgcggctc gttcccgtcg gagtgaggac cttcgaggat ctccacctcc ccaaggtatt
     1621 ggaaaagatc tccctggagg agcgggggct catcctctgc accgggacga ccgggtgtgg
     1681 caagtccacc acgcttgcgg cgatggtcca gtacatcaac acccatcgca gctgccacgt
     1741 catgacgatc gaggatccga tcgagttcct tctgcgcgac ggcaagagca tcatcaacca
     1801 gcgggagctc ggggtggact cgtcgtcgtt cgccgacgcc ctgaagagct ccctgcggca
     1861 ggatcccgac gtgatcctcg tgggggagat gcgcgatctt gagacgatcg agacggcgat
     1921 cgtcgccgcg gaaacggggc acctcgtact gtcgacgttg cacaccatgg acgccgccga
     1981 aacgatcaac cggatcgttg cgacgtttcc cccgttccag cagaagcaga tccggctgca
     2041 gttgggctcg gtcctcaagg cggtcgtttc gcagcggctg cttccccgcg cggacggcca
     2101 ggggcgcgtt cccgccgcgg agatcctgct gaacaccgcc cgggtccggg aatacatcga
     2161 ggacaaggac aagacccgca agatccgcga ggcgatcgcg cagggattcg tgagctacgg
     2221 gatgcagacc ttcgaccagt ccctcctgac gctcctcaag gaggggctcg tcaccctcga
     2281 cgaggtcctc cggcaagcca gcaaccccga cgacttcgcc ttgcgggtcc gtggggtctc
     2341 ctcgacctcg gacctcacgt gggacgattt tgaaaagggc agtcccccga agccctcata
     2401 aggcagactc cggccggccc ccgggcgggg gaatcgagct cgccatcggg ttgttggcgc
     2461 gccgcccctt gagcgaggga gaggtggccc agcggctcgc gcgcaagggg gtcgccgaag
     2521 gcgagatcct tccggtcctc tcgcgcctgc gcgagctggg cctgctcgaa gacgccgcgt
     2581 tgtgtggccg gctggcccgt tcatacttgg aagatcggcg gtacggtccc gcgaaaatcg
     2641 catggaagct cgcgtcgcgc cgcttctccc gggacctcgt cgaggaggcg cttcgggagg
     2701 tcgcttcccc cggggcggtg gccgaggccg ctgcgatcgc gcttcggaaa aagttccgga
     2761 acgggattcc tccggggcgg gagggggccg cgaaggcgta ccggtttctg gccgggcgcg
     2821 gctttccgcc cgacgcctgc cgggccgctg tcggccgggg gcggaccgaa ctatcccaag
     2881 gagactgatc catgtccata acgggtgcgc agctccgctc cggcttcctc gattatttcg
     2941 gaaagaacgg ccacaagatc ctgcccggcg cctcgcttgt ccctgcaaac gatccgaccc
     3001 tgctgttcac gaacgcagga atggtgcagt tcaaggagta tttcctcggg cagtcgacgg
     3061 ccgactggaa gcgggcgacc acggcgcagc ggtgccttcg ggtcagcggc aaacataacg
     3121 acctcgagaa cgtaggctac acggcgcggc accacacgct cttcgagatg ctggggaact
     3181 tctcgttcgg cgattacttc aagaaggagg cgatcttctt cgggtgggac ttcctcaccc
     3241 gcgaggtcgg cctcaatggc acgcggatga ccgtctccgt cttccgcgag gatgacgagg
     3301 cgtacgacct ctggcacaag acgataggga tccccgcctc gcggatcgtc cggttcgacg
     3361 agaaggacaa cttctggtcg atgggcgcca cggggccgtg cggcccatgc tccgaaatcc
     3421 tctacgatca gggggaggga gtcgggtgcg gccgcccggg gtgcgcggtg ggatgcgact
     3481 gcgaccggtt tctggaaata tggaacctcg tgttcatgca gttcaatcag gacgagtcgg
     3541 ggaagcggac tccgctgccg aagccgagca ttgacaccgg gatggggctc gagcggcttg
     3601 ccagcgtggt gcagggggtg accagcaact acgacacgga cctgttccgg ccgatcctcg
     3661 acgggatctc ccggcagtgc ggcgtgccca tcgggaaatc cccggctctc gacgcggcga
     3721 tgcgggtcgt cgccgaccat gcgcgggcca ccgccttcct catcgccgac gggatcgtcc
     3781 cttccaacga ggggcgcggg tacgtgctcc gccggatcat gcgccgggcg ctgcgccacg
     3841 ggaagaagct cgggtttgac ggccccttcc ttcacaaggt cgcgggaatc gtggtcgatg
     3901 agttccagga ggcgtacgcg ggtctcgccc agagcgcgtc gtttatcgac acggtggcgt
     3961 tccaggagga gcggcgattc ctcgagacgc tggacgcggg cctgcggatg gtcgaggagg
     4021 agttctcccg catgtccggg gatcgcaaga tcttccccgg cgaggtcgcc ttcaagctct
     4081 acgacacgta cgggttcccg gcggacctca ccgcggacct gtgccgggag cggggggtcg
     4141 ccctcgattc ggcggggttc gagaaggaga tggataaaca gcgcgcccag tccagggact
     4201 cgtggaaagg cggcgaggtc ggcgcgcagg acgccttggc cggcgaactc tcccgtgcgg
     4261 ggatctccgt cgacttcgtc gggtacgagc gaatggaatc ggacgcccac gtcgtggcgg
     4321 ttttccgcca gggggcgcgg gtcgcctccg cggcgtcggg cgaggaagcg gagttggtaa
     4381 ccgacgtgac gcccttctac ggggagtccg gcgggcaggc gggggacacc ggcgacgttc
     4441 agggggacgg cttcgccctc cgggtagagg acacccgcag ggtgtcctcc gaccaggttg
     4501 tccaccgggt caaggtcctt tcggggacgc tccgggaggg gcaggcggct cggctccggg
     4561 tagacgagac gtccaggcgg ctcacggagg ggaaccacac ggcgacgcac ctgctccagg
     4621 cggcgcttcg gaagctcctg ggcacccacg tgaagcaggc cggttcgtat gtcgggcccg
     4681 acaagctccg gttcgacttt tcccacttcg aagcgctctc tcccgaaacg ctttcagcgg
     4741 tcgaggagga ggtgaaccgg gtcgtcttct ccccccgtcc cgtcacgtgg gaatccctgc
     4801 cgtacgagca ggcggtggcg gcgggcgcga tggcgttctt cggggagaag tacgccgaca
     4861 tcgtccggat ggtgaccgtc ccggaggtga gtcgggaact gtgcggaggg acgcacgtgc
     4921 ggaacaccgc cgagatcggt ctcttccaca tcctttccga gggtgcggtg gccgccgggg
     4981 tccggcgcat cgaggcggtc acggggcttc ctgcctggcg gtgtctgcgg gaggaggccg
     5041 ggacgcttca cggcgtggcc cgggagttga aagtggcggc cccggaggta ccggagcggg
     5101 tgcgaaagct ttccgcgcag atcaaggcgt tggagaaagc gctccaggag gcccgccggc
     5161 ggtcgtcgcg ggaccgggtg ggggagatcc tctccggtgc gaaggaggtt gccggggtcc
     5221 ggcgtgtttc cgccgaggtg gaggcgatgg acgccgcggc gctgcgggat ctggccgacg
     5281 cggtgaaggg gaagctcttg agcggcatcc tgctcctggg agcccgggag ggggaacggt
     5341 gtcatctggt ggccggggta acttcggacc tcaccggtag gttctcggcg tccgacatcg
     5401 tccgaaaggc ggcggccctg gtgggcggcg gcggcggcgg ccggaaggac atggcacagg
     5461 ccggcggtcc ccgcgtcgat aatctgccgg aagcgcttgc ctcgatctct acgtgggaat
     5521 gagcacggga accgcgtgct tcaattcata attacgccaa catattagga ggcgctttga
     5581 tctccgcgag ataccccctg agatgctgca atatttagct gaacaataag gaacgggacg
     5641 cggatttatc cgggcgggtt ttcccggtcg atggtgttca ggtacacctg gacgatcttg
     5701 ttgagcggct gcttgtagtg cgcctcttcc cacgcgcggc gcgcccaacc cttctccccg
     5761 gcgaggtagt gcaggatccc cttctggatg agggcctccg ggttttccgg ggtctccgcc
     5821 agaaacgcgt ccacctcgtt gcgtgcctcg tccatccgcc ccgcttcacg gagcgccgtc
     5881 accagtcgaa cccgaaggtc ggggtatccc ggcgcgaccg agagggcgtg ccggtattcg
     5941 cggatcgcgt cgtcgtgctg gcccagcgca aggtagagtt ccccgagctc cttgtgcagg
     6001 ttcgcgattc tcccgcggaa cagattctcc ccgggcgacc ccccctttcg ggagaggatc
     6061 tccgaggcgc ggtcgtaggc atcccgcgcc tcgtcgtacc ggcccatgtc gttcaacgtg
     6121 atggagaggg aaagaagcgc ttcggtatag tcggggttga gggagaccgc ctgcccgaac
     6181 cgctcgaccg cccgccggta attcccccgc tggtggtcga tcaggcccag ggtgtaatgg
     6241 atgtcgggat atttcgcgcc gccctcgagg gcttcgagca ggtgccgctc ggcccgcaga
     6301 cagtccgcct cgctgaaggc gttctttccc atccggatca gcccggcaag ctcccggctc
     6361 accgtttctt ctccccgtgc ccggtatctt tctccgccag cttcttccgg aaggtctccg
     6421 cctcgtccct cttcccctgc ttttcgagcg cctgcacgag caaggtgagg agcttcgtgg
     6481 cggccggcac gtcggggtac gtcgcgaccg cccaacgggc ccgcgcctcg gcgctgtcgt
     6541 accgtttcct ggagaggaag ctggtcacca cgttcgcctc gtgcagggcc agccggtttc
     6601 tcagttcgag gatcttcccg gagatttccc gcgcgcgggg ggtgcccggc tccctctcga
     6661 ggaacagcgt atacgccttg atcgcctccc gcgtcttccc ctggtccctc ccgggcgcca
     6721 ggacctgccg tgaaagcagg ttccccttca tgaacagggc gtctgggatc ttcgggtccg
     6781 cggggtagag tcgcaggaaa tcatcgaacg ccacttcggc ctcgatctct tgcttgttgg
     6841 ccagccggtt cgaagcgaga ccgaactgcg
//
LOCUS       DASVJL010000372         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1751989, whole genome shotgun sequence.
ACCESSION   DASVJL010000372 DASVJL010000000
VERSION     DASVJL010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751989"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VF357_07865"
     CDS             complement(<1..1356)
                     /locus_tag="VF357_07865"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX7524102.1"
                     /translation="MLSILRRNAGSWAIKIILSFIALTFIWWGVGSYSERDRNVAATV
                     GGETITTNELSETVAGLEKTYREVYGSAFTPEMAKALNLKKQAMDSLVQRKILLAEAA
                     KMGLSATDEEVQREIAIIPAFQQNGQFRDDLYRSVLSYNRVTPAEFETSKRVEITMKK
                     VEGLIAAGALVPETEAKELFRVASRKVRLLVVTADPGRVKADAPTEGEILAKYEQAKE
                     RFRTPARVKLAVAAFTPDRFGREVDPSEAEIKTFYGTNSDRFRTEEQRLVSRIDLPFG
                     KKDRDAVRKKAEEILAKSSKGKTEFDAQVKAYDRGKAGETWLSRKDAGEALSGPLFQA
                     SVDTVVGPVELPGRFVLARVNRIRFPEALPLSQVRDRVVEQIRHEKGKDLAVVKAYEA
                     LPKATAAKSVKTTSAAFGVPVVESDWVGAEGAPGLPAALVQDALLLPSGEVAPVKTLG
                     DN"
     gene            1547..>1787
                     /locus_tag="VF357_07870"
     CDS             1547..>1787
                     /locus_tag="VF357_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7524103.1"
                     /translation="MFDRLLGLFSHDLAIDLGTATTLVYVKGEGIICSEPSAVAVHRD
                     TRGTKKVLAVGIEAKRMIGRTPGNIVAIRPIKDGVI"
BASE COUNT          284 a          581 c          576 g          346 t
ORIGIN      
        1 gttatccccg agggtcttca cgggggccac ctcgcccgag gggagcagca gcgcgtcctg
       61 gaccagcgcc gcggggaggc cgggcgcccc ttccgccccc acccagtcgc tctccacgac
      121 cggcacaccg aacgccgccg acgtcgtctt cacggacttc gcggcggtcg ccttcggcag
      181 cgcttcgtac gccttgacga cggccaggtc ttttcccttt tcgtgacgga tctgctcgac
      241 gacgcggtcg cgcacctggg agaggggaag cgcttccggg aaccggatcc ggttcacgcg
      301 ggcaagaacg aatctgcccg ggagctcgac cggcccgacg accgtgtcca cggaggcctg
      361 gaacagcggc ccggagagcg cctcgccggc gtccttgcgc gagagccacg tctctccggc
      421 tttcccgcgg tcatacgcct tcacctgggc atcgaattca gttttcccct tcgaagactt
      481 ggcgaggatc tcctccgcct tcttccgcac cgcgtccctg tcctttttcc cgaagggcag
      541 gtcgatgcgc gagaccagcc gttgctcctc ggtgcggaac cggtcggagt tcgtcccata
      601 gaacgtcttg atctcggcct ccgacgggtc gacttcccgg ccgaagcgat cgggagtgaa
      661 ggcggcgacg gcgagcttca cccgcgccgg ggtgcggaaa cgttccttcg cctgttcgta
      721 tttcgcgagg atctcccctt cggtcggagc gtcggccttc accctcccgg ggtccgccgt
      781 caccaccagg agccggacct tccgcgacgc gacccggaac agttccttcg cttccgtttc
      841 cgggacgagc gctccggcgg cgatcagacc ctcgaccttc ttcatcgtga tctcgacccg
      901 tttggaggtc tcgaactcgg ccggagtgac gcggttatag gagaggaccg atcggtacag
      961 gtcgtcccgg aactgtccgt tctgctggaa cgcggggata atggcgatct cccgctgcac
     1021 ctcttcgtcc gttgccgaca gtcccatctt cgcggcctcc gcgagcagga tcttccgctg
     1081 gaccagcgag tccatcgcct gcttcttcag gttgagcgcc ttggccatct cgggggtgaa
     1141 tgcggatccg tacacctccc gatacgtctt ctcgagtccg gcgacggtct cggacagctc
     1201 gttcgtcgtg atcgtttctc cgcccacggt ggcggccacg ttccggtccc gttcggaata
     1261 ggagccgact ccccaccaga tgaaggtcaa ggcgatgaag gagaggatga tcttgatggc
     1321 ccaggagccg gcgttgcggc ggagtatgga gagcatggcg aggaggttct cctgtcgatg
     1381 ggaatgaaag tgtcgtcgaa cgtgccattc taatgccgcg cgaggtccga tatcaacggg
     1441 atttcggccg gttccccctt cggattgcaa tgagggtcgg gggactgata agatacctcg
     1501 atacgtttcc gtgaacatcg aacgtgcgta tggaggatgc gggatgattt tcgaccggtt
     1561 gctcgggctg ttttcacatg atctggcgat cgacctcggc accgcgacga cgctggtgta
     1621 cgtgaagggg gaggggatca tctgctccga gccctcggcc gtggcggtcc accgggacac
     1681 ccgggggacc aagaaggtgc tcgccgtcgg gatcgaggcg aagcggatga tcggacgcac
     1741 gccggggaac atcgtggcga tccgccccat caaggacggc gtcatcg
//
LOCUS       DASVJL010000373         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1656865, whole genome shotgun sequence.
ACCESSION   DASVJL010000373 DASVJL010000000
VERSION     DASVJL010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656865"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..657)
                     /locus_tag="VF357_07875"
     CDS             complement(205..657)
                     /locus_tag="VF357_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524104.1"
                     /translation="MKKIFTVVMAVAFAFSVAGFAVAADNAAKPAKAAEKSATSAEKS
                     TKKAKGQTVTGTIEALDAAAGTFTVKGKKENVDLKAGEKVKLDSFKVGDKVVVKYADG
                     NASSVKAAKAAKAAKKAEPKGEAKPMIEKKAAPAAAPAAPPEIPAEKK"
     gene            complement(984..2420)
                     /locus_tag="VF357_07880"
     CDS             complement(984..2420)
                     /locus_tag="VF357_07880"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7524105.1"
                     /translation="MGLKSFEQHRLDLQDGREVYFEGERVEDVTTHPVLGAGMAFAAI
                     DYRLAEGKEHRELAVCTDPKSGEPYSRFYKIPETTEDLLKRRELIAAGSRVNGGPPLI
                     KEIGTDALFALHIVSDHMDKRLGTSVLPKVRHYFDTCRSRDLSFAVAQTDVKGDRGKR
                     PSEQHNPDVYVRVVDRRKDGIVVRGAKAHTTASPFADELIVLPTRAMAQADADYAVAF
                     AVPVATPGVKLIAEVSSHPGDNRFDYPVTTDLKMVDTVTIFDDVFVPWERVFLCGEWQ
                     YAGVMANAFVEFHRFTAASYKLPMCELFIGSAQLIAEYNGVANVTHVRDKIMELIAWT
                     ETTRGLLVAAAMEHRVVPPGIAVPSVTLTNIAKHHFARNYHAMVQNLQDIAGGLLVTA
                     PFRKDWENEATRGYLDHYLGGVEGVSGVQRVRAMKLIRDIAASGFGGYQEILSIHAEG
                     SLAAQRITILRDYDVKRCVDIVKSYLKD"
     gene            2558..3751
                     /locus_tag="VF357_07885"
     CDS             2558..3751
                     /locus_tag="VF357_07885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7524106.1"
                     /translation="MIPQRARTTLAASCIIHFLHDGCSDLLYVLFPVWAREFSLSFAQ
                     VGLLRTGYSGALALFQVPAGYLADRWGEPRILAAGTFLTAVGFFCVGTAGGFVPLLVF
                     LMIGGLGSGTQHPLSSSLVSRAYEAGRRRVAIGTYNFSGDLGKVTWPACVAFVTAWAG
                     WRWAARGIGLVVLATAVGIFFLLASLVGGPAGPQATSAADRAGTGWGIRNAWGFSLLS
                     TIHAIDNGSRTVFLTYLPFLLLGKGAKMDLMWLALGVTFAGGATGKFVCGLLAERWGI
                     IRTVLVTETATGLGILLLLALPVTPALFLLPILGIALNGTSSVLYGTVAELAEPERRS
                     RVYGLFYTIGIGTGALAPPLFGLLSDAVGVRVTLAVLAAVILATLPLAHLLRPAVEGK
                     PSHIS"
BASE COUNT          592 a         1332 c         1128 g          819 t
ORIGIN      
        1 aggttggggc tgggggggcg atttttcctt ggcacacccg cccgcaacga tcgagagggc
       61 gaatgccgcc gctgcgagta cggggaaccg ttttctcatc ttcatcctca ataggtggtt
      121 aaaaaaaagg gcaggggaat tgtgtccccc tgcccgtgcg tctcggaaac ctttgtgggg
      181 ccggaccgct tcccggtaaa acggttactt cttttccgct ggaatctcag gaggcgccgc
      241 aggagccgcc gcaggagccg ccttcttctc gatcatcggc ttcgcctcgc ccttcggttc
      301 tgccttcttc gccgctttgg ccgccttcgc cgccttcacg gacgaagcat tgccatcggc
      361 atacttcacg acgaccttgt cgccgacctt gaaggaatcc aacttcacct tctcgccggc
      421 cttcaggtct acgttctcct tcttcccctt caccgtgaag gtgccggctg ccgcgtcgag
      481 cgcctcgatc gtgccggtga ccgtctggcc cttcgccttc ttcgtcgact tctccgccga
      541 cgtggcagac ttctccgccg ccttcgccgg cttcgccgcg ttgtcggccg cgaccgcgaa
      601 tcccgcgacc gagaacgcaa acgcaactgc catcactacc gtgaaaatct tcttcatttc
      661 gtttcccctt tcagagggag tatttgaaat ccttgcgagt gtgtactgca tgcaaggtgc
      721 caactcgtct aactggaaca aattaatgat aaacaacagg ttttcataat tcatgttccc
      781 ctccccgcca cccgacttcc ccagaaggga caccactccc ctatatgggg aatccccccg
      841 ggatttcccg cgtcaacagc ctctctgcga atcccctatt tcccccccga aaaaggcggt
      901 tgtttgcacg gggtttacgt tcgtccggca tatccctgat cgtccacgcc taccgcacct
      961 cccggaaggg ggggcctccc gcctcaatcc ttcaggtacg acttcacgat gtcgacgcac
     1021 cgcttcacgt cgtaatcccg gagaatcgtg atccgctggg cggccaacga tccctccgcg
     1081 tggatcgaca ggatttcctg gtaccccccg aatcccgagg ccgcgatgtc ccggatcagt
     1141 ttcatggccc ggaccctctg cacccccgac acgccttcca cccctccgag ataatggtcc
     1201 aggtacccgc gcgtggcttc gttctcccaa tccttccgga acggagccgt gaccaggagg
     1261 cctcccgcga tatcctgaag attctggacc atcgcgtggt aattccgggc aaaatgatgc
     1321 ttggcgatgt tcgtcagcgt gacgctgggc acggcgattc ccggtggcac cacccggtgc
     1381 tccatcgcgg cggccaccag gagcccgcgc gtggtctccg tccatgcgat caactccatg
     1441 atcttgtcgc ggacatgggt gacattcgcc acgccgttgt actcggcgat caattgggcg
     1501 gagccgatga acagctcgca catcggaagc ttgtacgaag ccgcggtgaa ccggtggaat
     1561 tcgacgaagg cattggccat gacccccgcg tactgccact ccccgcagag aaaaaccctc
     1621 tcccatggaa cgaagacgtc gtcgaagatg gtcaccgtat ccaccatctt caggtccgtg
     1681 gtgaccgggt aatcgaagcg gttgtccccg gggtggctcg aaacctcggc gatgagcttt
     1741 acaccgggcg tggccaccgg tacggcgaag gcgacggcgt agtccgcatc cgcttgggcc
     1801 atcgcgcggg tcgggagcac gatcagttcg tcggcgaacg gggacgccgt ggtgtgcgcc
     1861 ttcgctcccc gcacgacgat gccgtccttg cgccggtcca ccacgcggac gtagacgtcc
     1921 ggattatgct gttccgaggg cctcttccca cgatccccct tcacgtccgt ctgcgccacg
     1981 gcgaagctca ggtcccgcga gcggcatgta tcgaaatagt gcctgacctt cggcagaacg
     2041 gacgtgccga gacgcttgtc catgtggtcc gagacgatgt gcagggcgaa gagggcgtcc
     2101 gtcccgatct ccttgatcag cggcggtccc ccgttgacgc gcgagccggc agcgatcagt
     2161 tcgcgccgct tcagaagatc ttccgtggtt tcggggatct tgtagaaccg gctgtacggc
     2221 tcgccggact tcggatcggt gcataccgcc agttcccggt gctccttccc ctccgccagg
     2281 cggtagtcga tcgccgcgaa ggccatcccg gcgcccagta ccggatgcgt cgtgacatcc
     2341 tcgacccgct ccccttcgaa atacacctcc ctgccgtcct gaaggtcaag ccggtgctgc
     2401 tcgaacgatt tcagtcccat cgcgctcccc cccctccgcc ggactccccg gccggccgtt
     2461 tccctttaga taaggcgcgg cgccggagag gttcctggcg cgtttcatcg cttcgggtgg
     2521 cttgacagtt tgaaaggcga ttgaaagaat cgccgtcatg atcccgcagc gtgctcgtac
     2581 cacgctcgcc gcaagttgca tcatccactt cctgcacgac ggatgctccg atctcctgta
     2641 cgttctcttc cccgtgtggg cgcgcgagtt ctccctctcc ttcgcgcagg tcggcctgct
     2701 gcgcaccgga tactcgggcg ccctcgccct tttccaggtc ccggcggggt acctggcgga
     2761 ccgctggggc gagccgcgca tcttggccgc cggaacgttc ctcacggccg tcggtttttt
     2821 ctgcgttggc accgcgggag gtttcgttcc gctcctcgtg tttctgatga tcggcggcct
     2881 gggctccggc acccagcatc cgctatcgtc ctcattggtc tcccgggcct acgaggcggg
     2941 gcgccgccgc gtggccatcg ggacgtacaa cttctcgggg gacctgggca aggtgacttg
     3001 gccggcctgc gtggcctttg tcaccgcgtg ggcgggctgg cgctgggctg cacggggcat
     3061 cgggctggtc gtcctggcca ccgcggtcgg catcttcttc ctcttggcgt cgctggtggg
     3121 cggccccgcg ggtcctcaag caacttcggc cgccgaccgc gccggcaccg ggtggggaat
     3181 ccggaacgcg tggggcttct cgctcctctc cacgatccac gccatcgaca acgggtcacg
     3241 aaccgtcttc ctcacctacc tgccgttcct cttgctcgga aagggcgcga agatggatct
     3301 gatgtggctg gcgttggggg tgacctttgc aggcggggcc acggggaagt tcgtctgcgg
     3361 gctccttgcc gagcgatggg gcatcatccg gacggtcctc gtcaccgaga ccgctacggg
     3421 cctcggcatt ctgctgctcc tggccctccc cgtgacaccg gcgctgttcc tcctgcccat
     3481 cctgggcatc gcactcaatg gaacctcatc cgtgttgtac ggcaccgtcg ccgagttggc
     3541 ggagccggag cgccgctcgc gcgtctacgg cctgttctac accattggca tcggcacggg
     3601 cgcgctggcg ccgccgctct tcggtctcct ctccgatgcg gtcggcgtcc gggtgacgtt
     3661 ggcggtcctg gctgcggtga tcctcgccac gctccccctc gcccacctcc tccgaccggc
     3721 cgtcgaggga aaaccctccc acatttcgta gcgcctcgca taaccccttt gtcgcccgga
     3781 aatgtccgtt gtttgcaacg gatttgcaat tcgttcggga ctctccagtg aaatttccct
     3841 cgtacgattt gtttatcagc gggggggagc t
//
LOCUS       DASVJL010000374         6473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_624884, whole genome shotgun sequence.
ACCESSION   DASVJL010000374 DASVJL010000000
VERSION     DASVJL010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624884"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1258
                     /gene="ftsA"
                     /locus_tag="VF357_07890"
     CDS             26..1258
                     /gene="ftsA"
                     /locus_tag="VF357_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX7524107.1"
                     /translation="MDTKTNDQVIAGLDIGSSQVTTVLGRKTQDGVEILGMGECPTEG
                     MRRGAVVNVDATVKSIRQSVTEAERMTGLTIESVFVGISGPLIKSFNSHAAISVKNER
                     EVTDADFTRVLEIARTVELPNNREILHVLTQEFIVDDMAGIKDPRGMTGIRLDARVHV
                     VTNDVPGARNLARCVEKADLDVESFVLSPLASAEAVLTPEEREVGVVLMDFGGGTVEI
                     VIFFNGSLRHTYVLPLGGSSITSDIAIGLKLPQSDAEILKVSSGCAMIQKVRRDELVE
                     LPGVGGRLPRPIRRQYLSEIIEPRAEEIFTLLRKEILRSGYEENLGAGVVLTGGGSKL
                     DGLTDLGERVFKLPFRRGNPIGIGGLVEVVNGPAFATAVGLVQYGAKASERVYRSAEA
                     PAGGGVIDRFKRYLSEFF"
     gene            1287..2489
                     /gene="ftsZ"
                     /locus_tag="VF357_07895"
     CDS             1287..2489
                     /gene="ftsZ"
                     /locus_tag="VF357_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX7524108.1"
                     /translation="MASTEQEARRNRIFSIVEENRCRAVIKVFGVGGGGGNAINTMIE
                     EGLQGVEFIAANTDAQALSRSLAPLKLQLGARLTKGLGAGANPDVGRQAALEDRDLIR
                     EALTGADMVFITAGLGGGTGTGAGPVVAEVAKEVGALTVAIVTRPFPFEGLTRKRQAD
                     GGTKELRSIVDTIIVIPNEKLLLIAGKEMRFVEAFRKVDDVLFQAVRGISELITKPGY
                     INLDFADVKTIMSGMGVALMGTGVASGQNRAVAAAEKAISSPLLEDVSIRGARGVLIN
                     ITAGTSLSLSEVNDAASLVREEASDEANIIFGTVIDETLGDELKVTVVATGFEPGVTE
                     GVLRNPRKGIKLVSRADDLQTPAFLRAAAPKTFEERLEDLPLIDKEAEIESLDEFEIP
                     TFLRRRVE"
     gene            2507..4231
                     /locus_tag="VF357_07900"
     CDS             2507..4231
                     /locus_tag="VF357_07900"
                     /inference="COORDINATES: protein motif:HMM:NF040068.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7524109.1"
                     /translation="MGRPRRGGAGPANEIFAARKDWGVGIRVALVYPNRYAAGMSNLG
                     FLLIYARINARPDALCERVFLSVDGRRRAPPRGPGAGSANSPIEGRTLESGRPLSSFD
                     VVAVSLSYENDLLNVPAILAAGGIPPFREDRAASGDRYPLVVAGGFAASLNPEPVGVF
                     ADAVVVGDGERAVDLLLDHGYGPAGDPGYRRELSAIPGVYVPGGYLPEYAESSTADRA
                     AGRLPVLRAIEPLPGFPASVAREVLDLRSLSPAPPVILSEETELGSMSLVETSRGCPG
                     MCGFCAAAHACPTFREFPLDRVRAAVAAAWPHRRKVGLIGAAVLDWGPFRELAREILD
                     RGGTVSPASVRADLVDEEIAEILARSGHRTVALAPECGDDRLRARVGKRVPDETFFSA
                     AEVLARSGIVSFKLYFLLGFPGVPREEEVGGIVRFIGTFRERVLEVARGVGRMGVVTS
                     VLSPFVPKPFTPLQWASMTPEEELKSREDAVSAIVRAIPNLRVSASTPWDAVVQGYLG
                     LSDRRVAAGLRTARRGKLPRSAPGLAGALSSVVFREKGGGEFLPWEVIDGGLRKGALR
                     ARYEAFFR"
     gene            complement(4236..4637)
                     /locus_tag="VF357_07905"
     CDS             complement(4236..4637)
                     /locus_tag="VF357_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX7524110.1"
                     /translation="MAFKTISVKTENAQQLVDITQQVRLVVRESGVKSGVCWIFIPHT
                     TAAVTINENADPNVRQDILNHLERVAPSDGKYLHPEGNSHAHIKSSLVGSSVAVIVEA
                     GQLVLGTWQSVFLCDFDGPRTRNVMVRILSG"
     gene            4788..5399
                     /locus_tag="VF357_07910"
     CDS             4788..5399
                     /locus_tag="VF357_07910"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX7524111.1"
                     /translation="MILASESPRRRELLAAVGVPFRVVPSGVDEIPHPGESPSRFVRR
                     AALDKGEAVALLHPSSFVLSADTIVVADGRILGKPRDRAEARRMLSRLAGREHKVYTA
                     ICLLCRERGFRDLGTEVTRVRFRPLTAAEIAAYARTGECDDKAGAYAAQGAGMLLIDR
                     VAGSFTNVVGLPMTRVVAMLARARLIRVTRSGPACYAFAGRAR"
     gene            5396..6118
                     /locus_tag="VF357_07915"
     CDS             5396..6118
                     /locus_tag="VF357_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX7524112.1"
                     /translation="MTPAGLPPGEGAPLGERAAWVRDRIADAERRSGRAAGSVKLIAV
                     SKTQPLAQVLEAHAAGLSVFGENYVQEAGEKIPALPGAEWHLIGRLQGNKVRRAVALF
                     SWIQTADSARRIREISRCAVESGNTSRVLIEVNLGGEGSKAGADPGDLRAILDDCFGL
                     PGVRVEGFMAIPPFSADPEGSRPHFARLRELRDGLAREIPGVVLGELSMGMSNDFEQA
                     IAEGATMVRVGTAIFGSRQGRS"
     gene            6123..>6473
                     /locus_tag="VF357_07920"
     CDS             6123..>6473
                     /locus_tag="VF357_07920"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7524113.1"
                     /translation="MALGFLGAGNMGEAMIRGLLSAKLRSPEQVVVYDAAPGRGEELR
                     RRFGVATARSVEDLLRSCDTVVVAVKPQVLSSVLSGISRDAARGKTFVSIVAGAKIAL
                     FEKALGEGAKIVRTM"
BASE COUNT          986 a         2007 c         2411 g         1069 t
ORIGIN      
        1 cgtcttcatg cggccggggg agtgaatgga cacgaaaacg aatgatcagg tgatcgcggg
       61 gctggacatc gggtcgagcc aggtgaccac ggtcctcgga aggaagacgc aggacggcgt
      121 ggagatcctc gggatggggg agtgcccaac ggaggggatg cggaggggcg ccgtggtgaa
      181 cgtcgacgcc acggtgaagt cgatccggca gagcgtcacg gaggcggagc ggatgaccgg
      241 actgaccatc gaatcggtct tcgtcgggat ctccggaccg ctcatcaagt cgttcaacag
      301 ccacgcggcc atctcggtga agaacgagcg cgaggtcacc gacgccgact tcacgcgggt
      361 cctcgaaatc gcgcgcacgg tggagcttcc caacaaccgg gagatcctgc acgtcctcac
      421 gcaggagttc atcgtcgacg acatggccgg gatcaaggac ccccggggaa tgaccggcat
      481 ccgcctcgac gcacgcgtgc acgtcgtgac caacgacgtc ccgggggcgc ggaacctggc
      541 gcggtgcgtg gagaaagcgg acctcgacgt cgagagcttc gtcctctccc cgctggcgtc
      601 ggccgaggcg gtgctcaccc cggaggagcg cgaggtcggc gtggtgctga tggacttcgg
      661 cggcgggacc gtggagatcg tgatcttctt caacggttcc ctgcggcaca cctacgtcct
      721 gcccctcggg gggagcagca tcacttccga catcgcgatc gggctgaagc ttccccagtc
      781 ggacgcggag atcctgaagg tctcctcggg atgcgcgatg atccagaagg tgcgccggga
      841 cgagctggtg gaactgccgg gggtgggtgg gcggcttccc cgtccgatcc gccgccaata
      901 cctcagcgag atcatcgagc cgcgcgcgga agagatcttc acccttctgc ggaaggagat
      961 ccttcggtcc ggctacgagg aaaacctcgg ggccggggtc gtgctcaccg gcgggggttc
     1021 gaagctggat ggcctcaccg acctcgggga gcgggtgttc aagctcccct tccggagggg
     1081 gaacccgatc gggatcggag gattggtcga ggtcgtgaac ggtcccgcgt tcgccaccgc
     1141 ggtggggttg gtccagtacg gcgcgaaggc ttcggagagg gtctaccggt cggcggaggc
     1201 accggcaggc ggcggggtga tcgaccggtt caagcggtac ctgtcggaat ttttctgata
     1261 cgaaacccgg gaaaaaggag gaacggatgg ccagcacgga gcaggaggca aggaggaacc
     1321 ggatattctc tatcgttgag gagaaccgct gccgcgccgt tatcaaggtg ttcggcgtgg
     1381 gcggcggcgg cggcaacgcc atcaacacca tgatcgaaga agggctgcag ggcgtcgaat
     1441 tcatcgccgc gaacaccgac gcgcaggcgc tgtcgcggag cctggcgcct ctcaagcttc
     1501 agttgggagc ccggcttacc aaggggctcg gcgcgggcgc caaccccgac gtcggccgtc
     1561 aggcggccct cgaggaccgc gatctgatcc gggaggccct caccggcgcg gacatggtgt
     1621 tcatcacggc gggcctgggc ggcggcacgg ggaccggcgc ggggccggtg gtcgccgagg
     1681 tggcgaagga ggtcggggcg ctgacggttg cgatcgtgac gcggccgttc cccttcgaag
     1741 gactcacccg gaaacggcag gcggacgggg gaacgaagga gctgaggagc attgtcgaca
     1801 cgatcatcgt catcccgaac gagaagctgc tcctgatcgc cgggaaggag atgcgcttcg
     1861 tcgaggcgtt ccgcaaagtg gacgacgtgc ttttccaggc ggtccgcggg atctccgaac
     1921 tgatcacgaa gcccggctac atcaacctcg atttcgccga cgtgaagacg atcatgtcgg
     1981 ggatgggcgt ggcgctgatg gggacggggg tggcctccgg ccagaaccgg gcggtcgccg
     2041 ccgccgagaa ggcgatctcg agcccgctcc tcgaggatgt ctcgatccgc ggagcccgcg
     2101 gtgtcctcat caacatcacc gccgggacgt ccctgtccct gagcgaggtg aacgacgcgg
     2161 cgagcctggt ccgcgaggag gcctcggacg aggcgaacat catcttcggg acggtgatcg
     2221 acgaaacgct cggggacgaa ctgaaggtca cggtggtagc gaccgggttc gaaccgggcg
     2281 tcacggaggg cgtgttgagg aacccgcgga aggggatcaa gctggtcagc cgggcagacg
     2341 atctgcagac cccggcgttc ctgcgggctg ccgcgccgaa gacgttcgaa gagcgcctgg
     2401 aggatctccc actgatcgac aaggaggcgg agatcgaatc gctcgacgaa ttcgagattc
     2461 ccacgttcct ccgccggcgg gtggagtagc cggcggaacg ggagtcgtgg gaaggccgcg
     2521 tcgcgggggc gccgggcccg caaacgagat cttcgccgcg cgcaaggatt ggggagtcgg
     2581 aattcgcgtc gcactcgtct accccaaccg gtacgcggcg ggaatgtcga acctcgggtt
     2641 tctcctgatc tacgcccgga tcaacgcgcg tccggacgcc ctttgcgagc gggtcttcct
     2701 atcggtggac gggcggcggc gcgccccgcc gcgggggccg ggggccggtt ccgcgaattc
     2761 gcccatcgaa gggaggacgc tcgagagcgg gcgccccctt tcctcgttcg acgtcgtcgc
     2821 cgtctccctc tcctacgaga acgaccttct gaacgttccc gcgatcctgg ccgcgggcgg
     2881 gatccccccg ttccgggagg atcgtgccgc ctccggcgac cgataccccc tcgttgtcgc
     2941 cggcgggttc gccgcgtcgc tcaatcccga gcctgttggg gtcttcgccg acgccgtggt
     3001 cgtgggcgac ggggagcgcg cggtggacct cctcctggat catggatacg gcccggcggg
     3061 ggatccgggc taccgaaggg aactctccgc gatccccggc gtgtacgtcc ccggggggta
     3121 cctgccggaa tacgcggagt cgtccaccgc ggaccgggcc gcgggcagac tcccggtgct
     3181 ccgggcgatc gaaccgctac cgggattccc cgcctccgtt gcgcgcgagg tcctcgatct
     3241 tcgttccctg tccccggctc cgcccgtcat cctgtcggag gagacggaat tgggcagcat
     3301 gtccctcgtc gagacttccc gcggctgccc gggaatgtgc gggttctgcg ccgccgccca
     3361 cgcctgcccc accttccggg agtttcccct cgaccgcgtg cgtgcggcgg tcgccgcagc
     3421 ttggccccac cgcaggaagg tggggctcat cggggccgcg gttctcgact ggggtccctt
     3481 ccgggagttg gcgcgggaga tcctcgaccg gggtgggacg gtttcgccgg cctccgtgcg
     3541 cgcggacctc gtcgacgagg agatcgcgga gatcctcgcg aggagcgggc atcgcaccgt
     3601 ggcgctggcg ccggagtgcg gcgacgaccg actgcgcgcg cgggttggga agcgggtgcc
     3661 ggacgagacg ttcttctccg ccgcggaagt cctggcccgt tcggggatcg tctcgttcaa
     3721 gctgtatttc ctgctcggct ttccgggggt gccgcgggag gaagaggtcg gggggatcgt
     3781 acggttcatc gggaccttcc gggagcgcgt tctcgaggtg gcgcgcgggg tcggccggat
     3841 gggtgtcgta acatcggtgc tctctccctt cgtcccgaag ccgttcacgc cgctgcagtg
     3901 ggcttcgatg accccggagg aggagctgaa gtcgcgggag gatgcggtga gcgccatcgt
     3961 ccgcgcgatt ccaaacttgc gggtctccgc ttcgacccca tgggacgcgg tcgtgcaggg
     4021 gtacctcggg ctatcggacc ggcgggtggc cgccggcctg cggacggctc gccgtggaaa
     4081 gctcccgcgc tccgcgcccg ggttggccgg ggcgctgtcg tctgtcgtct tccgggagaa
     4141 aggtggcggg gagtttctcc cctgggaggt gatcgacggg ggtctgcgaa agggcgcgct
     4201 gcgcgcccgc tacgaggcct tcttccgcta gggggtcagc ccgataagat ccgcaccatc
     4261 acgttccggg tgcgggggcc gtcgaagtcg cacaggaaga ccgactgcca ggttccgaga
     4321 accagctggc cggcctcgac gataacggcg acgctcgatc cgacgaggct ggacttgatg
     4381 tgagcgtggg aattcccctc gggatggagg tacttcccgt cggaaggggc gacccgctcg
     4441 aggtggttca ggatgtcttg ccggacgttc gggtcggcgt tctcgttgat ggtgaccgcc
     4501 gccgtggtat gggggatgaa gatccagcag acgccgcttt tcacgccgct ttcgcgcacc
     4561 acgaggcgga cctgctgggt gatgtcgacc agttgctgcg cgttttcggt tttcacgctg
     4621 atcgtcttga acgccatggc ggaactccgt gcggatcctc aagttgctac ctttttatca
     4681 cggagcggct ccggagggca agggagatgg cgtcgaatac gcaggaacgt ttgccgtgac
     4741 ccgcgggatt cccatataat gcattcccga tggtcccgtc ggcccgcatg atcctcgcct
     4801 ccgagtcacc ccgccgtcgg gagctactcg ccgccgtcgg cgttccgttc cgcgtggttc
     4861 cgtcgggggt cgacgaaatt ccgcaccccg gggagtctcc ttcgcggttc gtccgcaggg
     4921 ccgctctcga caagggagaa gcggtggctt tgcttcaccc gtcgtccttc gtcctgtcgg
     4981 cggacacgat cgtggtcgcg gacggccgga tcctcgggaa gccccgggac cgcgcggagg
     5041 cgaggcggat gctctcgcgc ctcgccggcc gcgagcacaa ggtgtacacg gcgatttgcc
     5101 tcctttgccg cgagcgcggc ttccgggacc tcggtacgga ggtgacccgg gtgcgattcc
     5161 gccccctcac ggcggcggag atcgcggcct atgcccggac gggggagtgc gacgacaagg
     5221 cgggggcgta cgcggcgcag ggggcgggga tgctgctcat cgaccgggtc gccggatcgt
     5281 tcacgaacgt ggtgggactc ccgatgaccc gcgtcgtggc gatgcttgcg cgggcgcggc
     5341 tgatccgggt cacgcgcagc ggcccggcgt gctacgcctt cgcgggtcga gcgcgatgac
     5401 cccggcaggg ttgcctccgg gagagggtgc gccgctcggg gaacgtgcgg cgtgggtgcg
     5461 ggaccggatc gcggacgccg aacgacggtc gggacgggcg gccggttccg tgaagctgat
     5521 cgcggtttcg aagacccagc cgctcgcgca ggtcctcgag gcgcacgcgg cggggctgtc
     5581 ggtgtttggc gagaattacg tccaggaggc gggggagaag atcccggcgc tccccggtgc
     5641 cgaatggcac ctgatcgggc gtctgcaggg gaacaaggtt cgcagggcgg tcgccctgtt
     5701 ttcctggatc cagacggcgg attccgccag gcggatccgc gagatctcgc gttgcgccgt
     5761 ggaatccggg aatacgtcac gggtcctgat cgaggtcaat ctcggtggcg aggggagcaa
     5821 ggcgggggcc gaccccggcg atctgcgcgc gattctcgac gattgctttg ggctcccagg
     5881 ggtccgggtg gaggggttca tggcgatccc gccgttctcc gccgaccccg aggggagccg
     5941 gcctcacttc gcgcggctgc gggaactgcg cgacgggctg gcgcgcgaaa tccccggcgt
     6001 ggtgctcggg gagttgtcga tggggatgtc caacgatttc gaacaggcga tcgcggaagg
     6061 ggcgacgatg gtccgcgtgg ggaccgcgat cttcggaagc agacagggga ggagctgata
     6121 tcatggcgct cggatttctc ggtgctggaa acatggggga ggcgatgatc cgcggcttgc
     6181 tctccgcgaa actgcgctcc ccggagcagg tcgtggtcta cgacgcggcg ccgggtcggg
     6241 gggaggagct gcgtcgccgc ttcggggtcg ccaccgcccg ttccgtcgag gacctgctgc
     6301 gctcctgcga cacggtggtg gtcgccgtga aaccgcaggt cctgtcgtcc gtcctgtccg
     6361 ggatctcccg ggacgcggcg cgggggaaga cgttcgtctc catcgtggcg ggcgcgaaga
     6421 tcgccctctt cgagaaggcg ctcggggaag gcgcgaagat cgtgcggacg atg
//
LOCUS       DASVJL010000375         2913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_813362, whole genome shotgun sequence.
ACCESSION   DASVJL010000375 DASVJL010000000
VERSION     DASVJL010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813362"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..1981)
                     /gene="nifA"
                     /locus_tag="VF357_07925"
     CDS             complement(323..1981)
                     /gene="nifA"
                     /locus_tag="VF357_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HEX7524114.1"
                     /translation="MTDPRSPEKAEIAAIHEVAKILTSTQNLDRALGTALRTLQSFLG
                     FDRTAIFRPDETTREIRMEVAAGYTAEQRERGRYVWGEGIVGKTMKTGSPIALPDVRV
                     EPSFLDKTRAHGTSSGEGPLSWICVPIKIGAETLGVLSAERIGREGTRALESDTRTLT
                     VIGCLIGQALKLHKAIERLQDEFKRQRNEFEKTIRKTYRIENIVGQSKRMQEVFAAVT
                     SVAPSRATVLLRGESGTGKEMIARAIHQGGGRADRPFIAVNCAALPETLLESELFGHK
                     RGAFTGAVEERKGRFEEASGGTIFLDEVGDIPLPTQVKLLRVLQERTFERLGENRPVP
                     VDVRIIAATNADLEKMVAGGTFREDLYYRLNVIPIFLPPLRDRKEDILPLTEHFLERF
                     NREHGKSVAFSKDALELLLEYRWTGNVRELENLVERAVVMAKAPVVRAQDLPRAIRVA
                     ASLPMTGSYGQPPAPQASVTAAAAEPPHPAAPREGRPRVEFLKAMEREELQTALASAG
                     WVIARAAKILGWTPRQVAYKMKKHGLSSPWKRDAALSPAPLPDR"
     gene            2062..>2913
                     /gene="speY"
                     /locus_tag="VF357_07930"
     CDS             2062..>2913
                     /gene="speY"
                     /locus_tag="VF357_07930"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF001980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEX7524115.1"
                     /translation="MAKRSRYLSGARILPPPVGKRMPASELIERTFLAYNAGRLREGA
                     RLLSERMLKPDVTVGLSLTGALTPAGLGVSCVVPLLKAGFVDWVVSTGANLYHDAHFG
                     LGLPMHAGSPFLDDRVLRDEGVVRIYDVLFDYEVLLDTDAFFRQVLDLPEFQAQMGTA
                     EFHYRLGRYMAQREKALGQGEVSVLAAAWRYGVPVYTSSPGDSSIGMNVAAMRLGGRG
                     VTFDVSLDVNETAAIVYDAKRRGGKSAVWILGGGSPKNFMLQTEPQIQEVLGLDEKGH
                     DYFLQITD"
BASE COUNT          409 a         1001 c          994 g          509 t
ORIGIN      
        1 ccacggctgc gctccggctt ccagggctcc attccctcag atccggcatt caagtccggc
       61 tgcgccgcct cggagggcag ggcttcgttc ttggctcgcc gtgcggtgaa cctgcacggc
      121 tgcgctttac ctcactccgc cctccctcct gcggcggctc cgccggatct tcttggtgca
      181 tagcctctgc ggcaccggaa ccctggaggc cggcctcact gtgccccccg ttcttcctac
      241 agcgggggta ccccagggag cgctatccat ccgggagtgg ggtgcccctc gaggtgttca
      301 tagcggggta ccccagggag cgctatctat ccgggagtgg ggcggggctc agcgcggcgt
      361 cccttttcca cggggaggaa agcccgtgct tcttcatctt gtacgccacc tgccggggcg
      421 tccacccgag gatcttcgcg gcccgcgcga tcacccagcc tgcggacgcc agcgccgtct
      481 gcaattcctc gcgctccatc gccttgagga actcgaccct gggtcggcct tcccgcggcg
      541 ccgccgggtg gggaggctcc gcggcggccg cggtgacgga cgcctgcgga gcgggaggct
      601 gtccatacga cccggtcatg gggagggacg cggcgacccg gatcgcacgg gggaggtcct
      661 gcgcccgcac gaccggcgcc ttggccatca ccaccgcccg ctcgacgagg ttctcgagct
      721 cccgcacgtt cccggtccac cggtactcga ggagcagctc gagggcgtcc ttcgagaagg
      781 cgacgctttt tccgtgctcc cggttgaacc gctcgaggaa atgctccgtc agcgggagga
      841 tgtcttcctt ccggtcccgc aaggggggga ggaagatggg gatcacgttg agccggtagt
      901 agaggtcctc ccggaacgtg cctcccgcca ccatcttctc gaggtcggcg ttcgtcgcgg
      961 cgatgatccg cacgtccacg ggaacgggcc ggttctcgcc gaggcgctcg aacgtgcgtt
     1021 cctgcagcac gcgcagcagc ttcacctgcg tgggcagcgg gatgtcaccc acctcgtcga
     1081 ggaagatcgt gcctccggac gcctcctcga accggccttt ccgctcctcg accgcgccgg
     1141 tgaaggcgcc ccgcttgtgg ccgaacagct ccgactcgag cagcgtctcg gggagggcgg
     1201 cgcagttcac cgcgatgaag ggacggtccg cgcggcctcc cccctggtgg atcgcgcggg
     1261 cgatcatctc tttccccgtc ccgctctccc cccgcagcag caccgtcgcg cgcgacggcg
     1321 cgacgctggt cacggcggcg aagacctcct gcatccgctt gctctgcccg acgatgttct
     1381 cgatccggta cgtcttccgg atcgtctttt cgaattcgtt ccgttgccgc ttgaactcgt
     1441 cctgcagccg ctcgatcgcc ttgtgcagct tgagcgcctg cccgatgagg cagccgatca
     1501 cggtgagggt ccgcgtgtcc gattcgagcg cccgggtgcc ctcgcggccg atcctctccg
     1561 cgctcagcac acccagcgtc tcggcgccga tcttgatggg gacgcagatc caggaaagcg
     1621 gaccttctcc ggacgacgtt ccgtgcgccc gcgtcttgtc caggaacgac ggctccacgc
     1681 ggacgtcggg gagagcgata gggctgcccg tcttcatcgt cttcccgacg atcccctcgc
     1741 cccagacata acggccccgc tcccgctgtt ccgcggtgta gccggcggcg acctccatcc
     1801 ggatctcgcg cgtcgtctcg tcggggcgga agatggcggt gcggtcgaat ccgaggaagc
     1861 tctggagcgt gcgcagggcg gtccccagcg cgcggtcgag gttctgcgtc gaggtgagga
     1921 tcttggccac ctcgtggatg gccgcgatct cggctttctc gggggagcgg ggatcggtca
     1981 cggcccgatt atagcgtata atgccgcaag ccgccgggcg aggctggtgc agcgtgagga
     2041 atcccgaagg agaaacggca catggcaaag agatcacggt acctgtccgg ggcgcggatc
     2101 cttccgccgc ccgttgggaa gaggatgccg gcctcggagc tgatcgagag gacgttcctc
     2161 gcctacaacg cggggaggct gcgggagggg gcgcggctcc tttccgaacg gatgctgaag
     2221 cccgacgtca cggtggggct ctccctgacc ggtgcgctta cccccgcggg gctcggcgtg
     2281 tcctgcgtcg tgccgctgct caaggcgggg ttcgtcgact gggtcgtctc cacgggtgcg
     2341 aacctgtacc acgacgccca cttcgggctc gggcttccga tgcacgccgg atcgccgttc
     2401 ctcgacgacc gcgttctgcg ggacgagggg gtcgtgcgga tctacgacgt cctgttcgac
     2461 tacgaggtgc tcctcgacac ggacgccttc ttccgccagg tgctcgacct gcccgagttc
     2521 caggcgcaga tggggacggc ggagttccac taccggctcg ggcggtacat ggcgcagcgg
     2581 gaaaaggccc tcggccaggg ggaggtgagc gtgctggcgg cggcgtggcg gtacggggtc
     2641 cccgtctaca cgtcgtcccc gggagattcc tccatcggga tgaacgtcgc cgcgatgcgt
     2701 cttggcggcc gcggggtgac gttcgacgtc tccctcgacg tgaacgagac ggcggcgatc
     2761 gtctacgacg ccaaacgccg cggggggaaa agcgcggtct ggatcctcgg cggcgggtcg
     2821 ccgaagaact tcatgctcca gacggagccg cagatccagg aggtcctcgg gctcgacgaa
     2881 aaggggcacg actacttcct tcagatcacc gac
//
LOCUS       DASVJL010000376         7824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1001410, whole genome shotgun sequence.
ACCESSION   DASVJL010000376 DASVJL010000000
VERSION     DASVJL010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001410"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VF357_07935"
     CDS             <1..768
                     /locus_tag="VF357_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7524116.1"
                     /translation="QVFSESIQSLTDRLWESGYQVLLGTSGYPTSREESLLAAILSRR
                     PDGIFLTGISHSAKSRRRLVAANIPIVEVWDLTPSPIDMLVGFSHEKVGQAVAEYLAG
                     RGHRHFGVISADDARAEVRRMGFLSVLEKNRIDDVQTVHVPAPSNLRLGREGLTRLLA
                     HGSLPRAVFCSSDTLAHGVLIEAQARGLSVPGDLAIVGFADLDFAAHTFPPLSTVWID
                     RPAVGRRAAESLLGRIEGRPVEKIVDIGFQIMERGTT"
     gene            784..2367
                     /gene="garD"
                     /locus_tag="VF357_07940"
     CDS             784..2367
                     /gene="garD"
                     /locus_tag="VF357_07940"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR03248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HEX7524117.1"
                     /translation="MPHDDDVSMNSSRERAFTIKIHDRDNVAIVVNTKGLPAGTRLDD
                     GTVLLDTVPQGHKVSLADMAEGSPVLRYGEVIGRAARSIARGTWIDESLVLLPEPPAL
                     DDLPLATSPAPDLPPLDGYAFDGYRNKDGSVGTKNVLGITTSVQCVAGVTDFLVHRIK
                     EELLPRYPNVDDVVALNHSYGCGVAIHAPDAIVPIRTLQNITRNPNFGGEVMIIGLGC
                     EKLEPERLVPTEENLACAGGPTDPLVLRLQDESFRGFGDSIAAMMEMAETRLSRMNRR
                     RRESCPASDLVVGVQCGGSDAFSGVTANPAVGYAADLIVRAGGTVMFSEVTEVRDAIH
                     LLTPRAIDESVGRALLHEMAWYDRYLERGGADRSANPTPGNKKGGLANVMEKALGSIV
                     KSGTSRIVDVLGPGERVRRKGLIFAATPASDFVCGTLQLAAGMTLQVFTTGRGTPYGL
                     PMVPVVKISSNTPLRDRWGDLIDLDAGRIATGEASIADVGWELFRLILDVASGRKQVA
                     ADRFGLHNSLVLFNPAPVT"
     gene            complement(2345..5005)
                     /gene="clpB"
                     /locus_tag="VF357_07945"
     CDS             complement(2345..5005)
                     /gene="clpB"
                     /locus_tag="VF357_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX7524118.1"
                     /translation="MPRAPSVLSVDNLPSVEATMISLDKLTVKSQEALQDAIRLASER
                     GHAEVEGEHLLCAFLRQEGGVMGELLARTGVSPQRLLSEAERLLASLPKVSGAVTRGL
                     SPRLEPLFRRALSEADAFKDEFLSAEHFLLAFASEPGKVADLLRKHGLTRESLLSALT
                     AIRGTQRITDENPEAKYQALEKYCRDLTDAARRDKLDPVIGRDDEIRRVIQVLSRRTK
                     NNPVLIGEPGVGKTAIAEGLAQRVVTGDVPEGLKEKRVLSLDLGALIAGAKYRGEFED
                     RLKAVLKEITAAEGKVILFIDELHTLVGAGKAEGSMDASNMLKPALARGELRCIGATT
                     LDEYRKYVEKDAALERRFQPVPVGEPSVEDTIAILRGLKGRYEVHHGVRIKDSALIAA
                     ATLSHRYISDRFLPDKAIDLVDEAASRLKIEIDSVPTEIDEVERRKIQLQIERQALLK
                     ETDPASRERLSGIESELSELSEKIAGLRGQWEEEKREIAGHRGVKERIEQTLQEMQRA
                     EREGNLEKASQLKYGALPALRKQVEEASQRAAECSKDRLLKEEVDEEDVAQIVSRWTG
                     IPVSRLVEGEVEKLLKMEERLSARVVGQEEAVGLVANAVRRARSGLKDPRRPIGSFLF
                     LGPTGVGKTELARALAQFLFDDEAAMVRLDMSEYMEKHSVARMIGAPPGYVGYEEGGG
                     LTEAVRRKPYAVILFDEIEKAHPDVFNILLQVLEDGRLTDGQGRTVDFRNAVIILTSN
                     VGSHLIQELVGKGEEVVRDRVMEELRRGFRPEFLNRLDEIVLFHSLGKEELSRIVDIM
                     VRELNDRLAERKIAIALTPAAKARIAEIGYDPAYGARPLRRALQKRIEDALAMRILEG
                     EFPEGDRVTVDVDAKGGFTFRREITSPAPG"
     gene            complement(5036..6511)
                     /locus_tag="VF357_07950"
     CDS             complement(5036..6511)
                     /locus_tag="VF357_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX7524119.1"
                     /translation="MIRKSKVVVLVLAAVAAGIVLSAGFNFSPIARALWGDKEKPVMA
                     PTAAGLRMVPVDIPQLFKGVSPAVVNISTTQVVKFNHPRMRNPFGQQDPFDDFFNNFF
                     GRMPREQKRKSLGSGFIVSSDGYILTNNHVVEKADEVTVTLLDKEEFKAKVVGTDPKI
                     DIALIKIDVKKKLPFVALGDSDKLEVGEWVVAIGNPFGLGHTVTAGIVSAKGRIIGSG
                     PYDDFIQTDASINPGNSGGPLFNLKGEVVGINSAIIQGGQGIGFATPIQLAKSVLEQL
                     KDKGKVTRGWLGVYIQRLAPETAEGLGISGRRGALVSDVTSGGPAEKAGIRSGDVIVG
                     FNGKEITDEHELPQAVATTKPGKSVEVRLLRDGKEITIAVTIAEMEGEAGKPPAGKAE
                     LSKNLGLTVQDITPEIAQRFEIENTKGVVVTGVADGSPAGNAGFNEGDIIRAILRQDK
                     RNPVTNAAEFAKLVKTFQSDKTILFLVERGEARILLTVKNK"
     gene            complement(6508..7056)
                     /locus_tag="VF357_07955"
     CDS             complement(6508..7056)
                     /locus_tag="VF357_07955"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEX7524120.1"
                     /translation="MPEEKEDKGFKVIDRRGVEPAPSPPPPPPLKENPPSAATEGKAR
                     ENTGASPPKHTHEDFPPAGAKEGNLSPPAEVQSGKGSAREGASSLGVPRFLDLVQSLQ
                     MGAMVGLGMLQGPDGKRPPVDLPAAKDAIDLLGILQEKTKGNLTKEEEEVLREGLYHL
                     RMGYMAMINAPPAGHGKGGDQR"
     gene            complement(7056..>7824)
                     /locus_tag="VF357_07960"
     CDS             complement(7056..>7824)
                     /locus_tag="VF357_07960"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7524121.1"
                     /translation="KTEMARALAEYLFGDERQMIRVDMSEYRDGALAVDKLIGMPRGI
                     VGSERGGILTNQVKDNPYSVVLLDEIEKADTYVHNLFLQAFDEGWLTDGRGKKVYFSD
                     TIIIMTSNLGAEELSKLTRPMGFGSGTVDFEPVRKAVLKAAENRFTPEFINRIDDVVV
                     FTPLSIDEVRRIAELYIDTIRKRMAAHGKTLVVSDAAVVALARAGFSVKYGARFLKRG
                     IDEKIKVPVTLKWKESDHFLVEEVDGEVVLITQKVAV"
BASE COUNT         1240 a         2683 c         2440 g         1461 t
ORIGIN      
        1 caggttttct ccgagtccat ccagtccctg accgatcgat tgtgggagag cggctaccag
       61 gtgctgctcg gcacatcggg ttaccccacg tcccgcgaag aaagccttct cgccgccatt
      121 ctcagcaggc gccccgacgg catattcctc accggcattt cgcattccgc gaaaagccgg
      181 cgtcgccttg tcgcggcgaa catcccgatc gtggaagtgt gggatctcac tccaagtccg
      241 atcgacatgc tcgtcgggtt ctctcatgag aaagtcggac aggcggtggc cgagtacctc
      301 gctggcagag ggcatcggca tttcggggtg atatcggccg acgacgcgcg cgcggaagtg
      361 cggcgaatgg ggtttctgtc ggttctcgag aaaaaccgga tcgacgacgt gcagaccgtc
      421 cacgtaccgg cgccgtccaa tctccgactg ggcagggaag ggctcactcg ccttcttgca
      481 cacggttccc ttccccgtgc ggttttctgc agttcggaca ctcttgccca cggagtgctg
      541 atcgaggcgc aggcgcgcgg tttgtccgtt ccgggggatc tggccatcgt cggtttcgcc
      601 gacctcgact tcgcggcgca cacctttcct ccattgtcga cggtgtggat cgaccggccc
      661 gccgtaggca ggcgagccgc ggaatccctg ttgggaagaa tcgaaggccg acccgtggaa
      721 aagatcgtcg acatcgggtt tcaaataatg gaacgcggga cgacctgacg aggggaaatc
      781 ggcatgccgc acgacgacga tgtctcgatg aattcgtccc gggaacgggc gttcacgatc
      841 aagatccatg atcgggacaa cgtggcgatc gtcgtcaaca ccaaagggtt gccggcaggc
      901 acgcggcttg acgacggcac cgtcctgctc gacaccgttc cacaaggcca caaggtttcg
      961 ctcgccgaca tggcggaagg ttcgccggta ctgcgatacg gagaggtgat cggacgtgca
     1021 gccaggtcga tcgcgcgcgg aacctggatc gacgagtcgc tcgtcctcct gcccgagcct
     1081 ccggcgctcg acgatcttcc gctcgcgacg tctccggcgc ccgacctgcc gccgctcgac
     1141 ggatacgcct tcgacggcta tcgcaacaag gatggctccg tcggcacgaa gaacgtgctt
     1201 ggcatcacca ccagcgtgca gtgcgtcgcc ggcgtcacgg acttcctggt acatcgcatc
     1261 aaggaagagc tgctgccccg ctatccgaac gtcgacgacg tggtcgcgct gaaccattct
     1321 tacggctgcg gcgtcgcgat ccacgccccc gacgccatcg tgccgatccg caccttgcag
     1381 aacatcacgc gcaatccgaa tttcggcggc gaggtgatga tcatcggtct cggctgcgaa
     1441 aaactggagc cggagcggct tgtacccacg gaggagaacc tggcctgcgc cgggggaccc
     1501 acggacccac tcgtcctgcg tctccaggat gaatcgttcc gcggcttcgg tgattcgatc
     1561 gccgcaatga tggagatggc cgaaacgcgg ctgtcgcgga tgaatcgccg gcgtcgggag
     1621 tcgtgccccg cttccgacct ggtcgtcggc gtgcaatgcg gcggcagcga tgcgttttcc
     1681 ggcgtcacgg ccaatcccgc ggtggggtac gcggcggacc tcatcgtgcg ggcgggcggt
     1741 accgtgatgt tctcggaagt gacggaggtc cgcgacgcga tccatctgct caccccccgc
     1801 gcgatcgacg aatccgtcgg acgggcattg ctgcatgaaa tggcctggta cgatcgctac
     1861 ctggagcgtg gcggggcgga ccgcagcgcc aacccgacgc cgggaaacaa gaagggcgga
     1921 ctggcgaacg tgatggaaaa ggcgctcggc tcgatcgtca agtccggaac cagccggatc
     1981 gtcgatgtgc tcggacccgg cgagcgcgta cggcgaaaag gcctcatctt tgcggcgacc
     2041 ccggccagcg acttcgtttg cggtacgctg caacttgcgg cgggcatgac cctgcaggtg
     2101 ttcacgaccg gccggggcac tccctacggt ctgccgatgg tcccggtggt gaagatttca
     2161 tccaatacgc cgttgcgcga ccgctgggga gacctcatcg atctcgacgc cggacgcata
     2221 gcgaccggag aggcgagcat cgccgatgtc ggctgggagc tgttccgcct gattctcgac
     2281 gtcgcgagcg gaagaaagca ggtcgccgcc gaccggttcg ggctccacaa ttccctggtc
     2341 ctgttcaacc cggcgccggt gacgtgatct cccgtcggaa ggtgaagccc cctttcgcgt
     2401 cgacgtcgac cgtcacccgg tccccttcgg ggaactcccc ctcgaggatg cgcatcgcga
     2461 gggcgtcctc gatccgcttc tggagggcgc gccgcagcgg cctggccccg taggcggggt
     2521 cgtagccgat ctccgcgatc cgtgccttcg ccgccggggt gagggcgatc gcgatcttcc
     2581 tttccgcgag ccggtcgttc agttcccgca ccatgatgtc cacgatccgg gagagctcct
     2641 ccttcccgag ggagtggaaa aggacgatct cgtcgagccg gttcaggaac tcgggccgga
     2701 agccgcggcg aagttcctcc atcacgcggt cgcggaccac ctcttctcct ttccccacga
     2761 gctcctggat caagtgggag cccacgttgg aggtgaggat gatcaccgcg ttgcggaagt
     2821 ccacggtccg tccctgcccg tcggtcagcc gaccgtcctc gaggacctgc agcaggatgt
     2881 tgaagacgtc ggggtgcgcc ttttcgatct cgtcgaacag gatcaccgcg tatggcttgc
     2941 ggcggaccgc ctcggtgagg cctcccccct cctcgtaccc cacgtacccc ggcggcgctc
     3001 cgatcatccg cgcgaccgaa tgtttctcca tgtactcgga catgtcgagg cgcaccatgg
     3061 cggcctcgtc gtcgaacagg aactgcgcga gggctcgcgc gagctccgtc tttccgacgc
     3121 cggtcggccc gaggaagaga aacgacccga tggggcggcg gggatccttc agccccgacc
     3181 tggcgcgccg cacggcgttg gccacgagcc cgaccgcctc ctcctggccc accacccttg
     3241 cggaaaggcg ctcctccatc ttgaggagtt tctcgacctc cccctcgacc aaccgggata
     3301 cggggatccc cgtccaccgg gagacgatct gcgccacatc ctcctcgtcc acctcctcct
     3361 tgagaaggcg gtccttcgag cactccgccg cccgctgcga ggcctcctcg acctgctttc
     3421 gcagggcggg gagggcgccg tacttcagtt gagacgcctt ctcgaggttc ccctccctct
     3481 ccgcccgctg catctcctgg agggtctgct cgattcgctc cttgaccccc cggtgcccgg
     3541 cgatctcccg cttctcctcc tcccactgcc cgcgcaaccc ggcgatcttc tcggatagct
     3601 ccgacagctc cgactcgatc cccgaaagcc gctcccgcga cgccgggtcg gtctccttca
     3661 gcagcgcctg ccgctcgatc tggagctgga tcttgcgacg ctccacctcg tcgatctccg
     3721 tcgggacgga gtcgatctcg atcttcaggc gggacgccgc ctcgtccacc aggtcgatcg
     3781 ccttgtcggg gagaaaccgg tcggaaatgt accggtggga caaggtggcg gcggcgatca
     3841 gcgcggaatc cttgatccgc acgccatggt gcacctcgta gcggcctttg aggccccgga
     3901 gaatggcgat cgtgtcctcc acggacggct cgcccaccgg gaccggctgg aaccgccgct
     3961 ccagcgccgc gtccttctcc acgtacttgc gatattcgtc gagggtggtc gcgccgatgc
     4021 accgcagctc cccgcgcgcg agggcgggct tcagcatgtt ggaggcgtcc atcgacccct
     4081 ccgccttccc cgcccccacg agggtgtgca gttcgtcgat gaagaggatc accttcccct
     4141 cggcggcagt gatctccttc aggaccgcct tcaaccggtc ctcgaactcg ccccggtatt
     4201 tcgcgccggc gatgagcgcc ccgagatcga gggagagcac ccgcttctcc ttcagcccct
     4261 ccgggacgtc ccccgtcacc acccgctggg cgagcccctc cgcgatcgcg gtctttccca
     4321 cgccgggttc accgatgagg acggggttgt tcttggtacg gcgggagagg acctggatca
     4381 cgcggcggat ctcgtcgtcc cgcccgatca ccgggtcgag cttgtcgcgc cgggccgcgt
     4441 cggtcaggtc acggcaatac ttttcgaggg cctggtactt cgcctcgggg ttctcgtcgg
     4501 tgatccgctg ggtcccgcgg atggcggtca acgcggagag gagcgactcg cgcgtcaatc
     4561 cgtgtttccg aaggaggtcg gccacctttc cgggctcgga cgcgaaggcg aggaggaagt
     4621 gctcggcgga aaggaattcg tccttgaacg cgtccgcttc ggagagggcg cgccggaaca
     4681 gcggctcgag gcgaggcgac agcccccggg tcaccgcacc ggacaccttc ggcagcgacg
     4741 caagcagcct ctcggcctcg gaaagcaacc gttgggggga gaccccggtg cgggcgagga
     4801 gttcccccat gaccccgcct tcctgccgca ggaaagcaca gaggaggtgc tccccctcca
     4861 cttccgcgtg gccgcgctcc gatgcgagcc gtatcgcgtc ctgcaacgct tcctgggact
     4921 tcacggtgag cttgtcgagg gagatcatcg tcgcctccac ggacggaagg ttgtcgacgg
     4981 aaaggacgga gggcgcccgc ggcaccgcgc ggacgccccc ggatgtgcgt ttttcctact
     5041 tgtttttgac cgtcaggagg atgcgggctt cgccccgctc caccaggaag agtatcgtct
     5101 tgtcggactg gaacgtcttc acgagcttcg cgaattcggc cgcgttggtc accgggttcc
     5161 gcttgtcctg gcgcaggatc gcccggatga tatccccctc gttgaacccg gcgtttcccg
     5221 ccgggctgcc gtccgccacc ccggtcacca cgacgccctt cgtgttctct atctcgaacc
     5281 gctgcgcgat ctcgggggtg atgtcctgga cggtgagccc gagattcttc gagagctcgg
     5341 ccttcccagc aggaggcttc ccggcctccc cttccatctc ggcgatcgtc accgcgatgg
     5401 tgatctcctt cccgtcccgg agaagccgca cttccacgct tttccccggc ttcgtcgtgg
     5461 ccacggcctg cggcaattcg tgctcgtccg ttatctcctt gccattgaac cccacgatca
     5521 cgtcgccgga gcggatcccc gccttctccg ccgggccgcc cgaggtcacg tcggacacca
     5581 gcgccccgcg ccggccggaa attccgaggc cctcggccgt ctcgggggcg agccgctgga
     5641 tgtacacccc gagccacccg cgcgtcacct tccctttgtc cttcaactgc tcgaggaccg
     5701 acttggcgag ctggatcggc gtcgcgaacc cgatcccctg cccgccctgg atgatcgccg
     5761 agttgatccc gacgacctcg cctttcaggt tgaagagggg gccgcccgag ttccccgggt
     5821 tgatcgaggc gtcggtctgg atgaagtcgt cgtagggccc ggagccgatg atccgcccct
     5881 tggcgctgac gatccccgcc gtcacggtgt ggccgagccc gaacgggtta ccgatcgcca
     5941 cgacccactc gcccacttcg agcttatcgg agtcgcccag ggccacgaag gggagcttct
     6001 tcttcacgtc gatcttgatc agggcgatgt cgatcttcgg atcggtcccg acgaccttcg
     6061 ccttgaactc ctctttgtcg aggagggtga ccgtcacctc gtcggccttc tccaccacgt
     6121 ggttgttcgt gaggatgtag ccgtcggagg agacgatgaa cccggacccg agcgacttgc
     6181 gcttctgctc cctcggcatg cgcccgaaga agttgttgaa gaaatcgtcg aacggatctt
     6241 gctggccgaa cggattgcgc atccgggggt gattgaactt caccacctgg gtggtggaga
     6301 tgttgaccac cgccggggag acccccttga agagctgcgg gatgtcgacc gggaccatcc
     6361 gaagccccgc cgccgtggga gccatcaccg gcttttcctt gtcgccccac aaggctcggg
     6421 cgatcggcga gaagttgaat ccggccgaca gtacgatccc ggcggctacc gccgcaagaa
     6481 cgaggaccac aaccttgctt ttgcggatca ccgttgatct cctccctttc catgccccgc
     6541 ggggggcgcg ttgatcatcg ccatgtaccc catccggagg tggtacagac cctcgcggag
     6601 gacctcttcc tcttccttcg tcaggtttcc cttcgtcttc tcctgcagaa tcccgagaag
     6661 gtcgatggcg tccttcgccg ccggcaggtc gaccgggggg cgtttcccgt cgggtccctg
     6721 gagcatgccg agaccgacca tcgctcccat ctgcagggat tgcacgagat cgaggaaccg
     6781 ggggacgccc agcgaagacg cgccttcccg cgcactgccc tttccacttt ggacctccgc
     6841 aggaggagaa aggttcccct ccttcgcacc cgcggggggg aaatcttcat gcgtgtgctt
     6901 cggagggctt gcgcccgtat tctcccgggc cttgccctcc gtagccgccg acggaggatt
     6961 ttccttgaga ggcggaggag gagggggcga aggcgccggt tccacccccc tccggtcgat
     7021 caccttgaat cccttgtctt ctttttcttc cggcatcaga cggccacctt ctgcgtgatg
     7081 aggaccacct ccccgtcgac ctcctcgacg aggaagtgat cggactcttt ccacttcaac
     7141 gtgacgggca ccttgatctt ctcgtcgatt ccccgcttga gaaaacgcgc gccgtacttg
     7201 acggagaacc ccgcgcgggc aagtgcgacg acggccgcgt cggagacgac cagcgtcttc
     7261 ccatgcgcgg ccatcctctt ccggatcgtg tcgatgtaca gctcggcgat ccgccggacc
     7321 tcgtcgatgg agagaggcgt gaagacgacg acgtcgtcga tgcggttgat gaactccggc
     7381 gtgaaccggt tctcggccgc tttcagcacc gccttgcgga ccggctcgaa atcgaccgtc
     7441 cccgacccga accccatcgg tcgggtgagc ttcgacagct cctccgcccc gaggttgctc
     7501 gtcatgatga tgatcgtgtc ggagaagtag accttcttcc cgcgcccgtc ggtcagccac
     7561 ccctcgtcga acgcctggag aaagaggttg tggacgtagg tgtcggcctt ctcgatctcg
     7621 tcgagaagga ccacggagta cgggttgtcc ttcacctggt tcgtcaggat ccccccgcgt
     7681 tcggacccga cgatcccgcg cggcatcccg atcagcttgt cgaccgcgag cgcgccgtcg
     7741 cggtactcgg acatgtcgac gcggatcatc tgccgttcgt cgccgaagag gtactccgcc
     7801 agcgccctgg ccatctccgt cttg
//
LOCUS       DASVJL010000377         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1190823, whole genome shotgun sequence.
ACCESSION   DASVJL010000377 DASVJL010000000
VERSION     DASVJL010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190823"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VF357_07965"
     CDS             complement(<1..1144)
                     /locus_tag="VF357_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7524122.1"
                     /translation="MATVAKSYRLYIGGKWEGGKETMPVIDKFTGETIGLVPLADKAA
                     VDRAIGAAHAAFPSWSRTPAHKRYQILSKACQLFEKYQEEIAAIICREAGKAWKYSLG
                     EVSRSIETFRFSAEEAKRIHGETVPMDASAAGEGRVGFWLRTPLGVVSAITPFNFPLN
                     LVAHKVGPALAVGNTIVLKPASTTPLTALRLAEIFEEAGLPPGVLNVVVGSGGTVGEW
                     LTVDPRISKISFTGSPPVGEQIIRKAGLKKVTMELGNNSGTIIEPDADLSAAVPRCVM
                     SSFANSGQVCISLQRLYVHKAVAKEFTKRFVEETKKLKVGNPLEKDCDVGPMIEEKEA
                     IRAEEWVKEAVKEGAKVLIGGKREGRVMQPTVLVNVRSEMKVMCQEA"
     gene            complement(1181..2344)
                     /locus_tag="VF357_07970"
     CDS             complement(1181..2344)
                     /locus_tag="VF357_07970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX7524123.1"
                     /translation="MDQNWIFRTTPRIVFGCGSVGQAGTEAKAAGISRALLVTDPGVK
                     AAGISATVEKALLSAGIEVVVFDAVESDPRIEVVEKCASAARDGKCDGVVAVGGGSAL
                     DIGKLAAVMRKNEGPIGKYFGIDLVPSPGLPTVLLPTTSGTGSEVTPIAVLSDEGEHL
                     KKGVVSPYLFAKVAVLDPALTLGCPPGVTAASGMDALIHGIESYTSIHASKFTEFLAY
                     KAVETIAGSLRTAYANGADLEARTRMMEGSLWAGMAFANSGVAAVHAFAYPLGGEYHV
                     PHGIANTVMLPYVMRYNMVGALKRYADLAPALGAAECRCGMTDSERAEAAVAAVERLA
                     DDIRVPRRLRDVNIPESGIPVMAEGVMKVTRLLANNPRTMTVQDAEAIYRSAY"
BASE COUNT          379 a          924 c          738 g          452 t
ORIGIN      
        1 acgcctcctg gcacatcacc ttcatctcgg agcgaacgtt caccagcacc gtcggctgca
       61 tcacccgccc ctcgcgcttc ccgccgatca gcaccttcgc cccctccttg accgcctcct
      121 tcacccactc ctccgcccgg atcgcctcct tctcctcgat catcgggccc acgtcgcaat
      181 ccttttcgag cgggttcccg accttcagct tcttcgtctc ctcgacgaac cgcttcgtga
      241 actccttagc cacggccttg tgaacgtaga gccgctgcag ggaaatgcac acctggccgg
      301 agttggcgaa ggagctcatc acgcaccggg gaaccgccgc tgaaaggtcg gcgtccggct
      361 cgatgatcgt gccggagttg ttccccagct ccatcgtcac ttttttcagt cccgccttgc
      421 ggatgatctg ctccccgacg gggggcgacc cggtgaagga gatcttcgag atccgcgggt
      481 cgacggtcag ccactcgccc accgtgcccc ccgacccgac gacgacgttg agcaccccgg
      541 gagggagccc cgcctcctcg aagatctccg ccagccggag ggccgtcagc ggcgtggtgg
      601 acgcgggctt caggacaatc gtgttcccga ccgccagcgc cggccccacc ttgtgcgcca
      661 ccaggttcag ggggaagttg aagggggtga tcgcggaaac cacgcccagg ggggtgcgca
      721 gccagaaccc gacccggccc tcgccggccg cgctggcgtc catcggcacc gtctcgccgt
      781 ggatccgctt cgcctcctcc gccgagaacc ggaacgtctc gatcgaccgg gaaacctccc
      841 cgagggagta tttccacgcc tttcccgcct cgcggcagat gatcgcggcg atctcctcct
      901 ggtacttctc gaagagctgg cacgccttgc tcaggatctg gtaccgcttg tgggcggggg
      961 tccgcgacca ggaggggaac gcggcgtggg cggccccgat cgcacggtca accgccgcct
     1021 tgtcggcgag ggggactaac ccgatcgtct cgccggtgaa cttgtcgatg accggcatcg
     1081 tctccttgcc gccttcccac ttcccgccga tgtacagccg gtaggacttc gccacggtag
     1141 ccataatgtt tcgctcctcc tttggatttt cggttacggg tcagtacgcg cttcggtaga
     1201 tggcctctgc gtcctggacg gtcatcgtcc gcggattgtt cgccaacagc cgggtcacct
     1261 tcatcacgcc ttcggccatg acggggattc ccgactccgg aatgtttacg tcccgcagcc
     1321 gtctcggcac ccggatgtcg tcggccaacc gctccacggc cgcgaccgcc gcctcggccc
     1381 gttccgagtc ggtcatcccg caccggcact ccgcggcgcc cagcgcgggg gcgaggtcgg
     1441 cgtatcgctt cagcgccccg accatgttgt accgcattac gtacggcagc atcacggtgt
     1501 tcgcgattcc gtgcgggacg tggtactccc cgcccagcgg gtaggcgaag gcgtggaccg
     1561 cggccacccc ggagttcgcg aacgccatcc cggcccacag gctcccttcc atcatccgcg
     1621 tccgggcctc cagatccgcc ccattggcgt aggcggtgcg caggctcccc gcgatcgtct
     1681 ccaccgcctt gtacgcgaga aattcggtga acttgctcgc gtggatcgag gtgtacgact
     1741 cgatcccgtg gatgagagcg tccatgccgg acgccgccgt cacgcccgga gggcacccga
     1801 gcgtcagcgc cgggtcaagg accgcgacct tcgcgaacag gtacgggctc accacccctt
     1861 tcttcagatg ttctccctcg tcggacagga cggcgatggg agtcacctcc gacccggtcc
     1921 ccgacgtggt gggaagcagg accgtgggca acccggggga ggggacgagg tcgatcccaa
     1981 aatatttccc gatgggtccc tcgttcttcc gcatcaccgc ggccagcttc ccgatgtcga
     2041 gggcgctccc gcccccgacg gccaccacgc cgtcgcattt cccgtcgcgg gcggccgaag
     2101 cgcacttctc gacgacctcg atccgggggt cggactccac ggcgtcgaac acgacgacct
     2161 cgatccccgc ggagagaagc gccttttcca ccgtggcgct gattcccgcc gccttgaccc
     2221 ccgggtccgt caccagcagg gcgcgggaga tccccgccgc tttcgcctcg gtccccgcct
     2281 ggccgaccga cccgcacccg aaaacgatcc tcggcgtcgt ccggaatatc cagttctggt
     2341 ccattccttc cccccctcgc ggaaacgtct cagacggcaa ggtaccgttt ctgcacccga
     2401 tcgtcctcga cgaatccttt gatgtcgttt ctgtagcgga tggcgccgtt gtcgatgatg
     2461 aacacgcggt cggacacctt gaccgcgaac ttc
//
LOCUS       DASVJL010000378         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_344715, whole genome shotgun sequence.
ACCESSION   DASVJL010000378 DASVJL010000000
VERSION     DASVJL010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344715"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /gene="serS"
                     /locus_tag="VF357_07975"
     CDS             <1..979
                     /gene="serS"
                     /locus_tag="VF357_07975"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX7524124.1"
                     /translation="AIPNIPDPSVPDGAGEEDNPVVRTWGTPRAFSFPVKDHVDLGAA
                     LDILDFDRAAKIAGGRFCLSKGAGALMERALINFMLDVHTREHGYLEVLPPFMANSAS
                     FFGTGQLPKFEEDLFRVAGTDYFLVPTAEVPVTNIVRDEILAAETLPLKMTAYTPCFR
                     AEAGSYGKDTRGMIRQHQFNKVEMVKICRPEESAQELEKLTDDAEEILRRLDLPYRVV
                     TLCTGDLGFSSAKTYDIEVWVPSQERYREISSCSTFTDFQARRAKIRFREGASGKTRL
                     AHTLNGSGLAVGRTVVAILENYQREDGTVDVPSTLRPYMGGLARITKRC"
     gene            1022..1411
                     /locus_tag="VF357_07980"
     CDS             1022..1411
                     /locus_tag="VF357_07980"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX7524125.1"
                     /translation="MFVPFAGALFPTAFAAFCLYFATGAREFEAGAFVCALFGLLAAP
                     ATAATGFLDWWTHYKAYMTSVFRIKIAGAVVLMALAAPAVLLRVFHPDLAVLPLGGLG
                     WVYFGLLAACTATCVVLGYYGGKLVFH"
BASE COUNT          265 a          507 c          528 g          273 t
ORIGIN      
        1 cgcgattccg aacatcccgg atccctcggt cccggacggc gcgggggagg aggacaaccc
       61 cgtcgtgcgg acgtggggga ccccgcgggc cttctctttc ccggtcaagg accacgtcga
      121 cctcggcgcg gcgctggaca tcctcgattt cgaccgggcg gcgaagatcg ccggcgggcg
      181 gttctgcctc tcgaaggggg cgggggcgct gatggagcgg gcgctcatca acttcatgct
      241 cgacgtccac acgcgggagc acggatatct cgaggtgctt ccccccttca tggcgaacag
      301 cgcgtcgttc ttcggcaccg gacaactccc caagttcgag gaggatctct tccgcgtggc
      361 ggggaccgac tacttcctcg tccccaccgc ggaggtgccg gtgacgaaca tcgtgcggga
      421 cgagatcctc gccgccgaga cgcttcccct gaaaatgacg gcgtacaccc cgtgcttccg
      481 ggcggaggcg ggatcctacg gcaaggacac gcgggggatg atccggcagc accagttcaa
      541 caaggtggag atggtcaaga tctgccgccc ggaggagtcc gcgcaggagc tggagaagct
      601 caccgacgat gccgaggaga tccttcggcg cctcgacctg ccgtatcgcg tggtgacgct
      661 gtgcacgggg gacctcgggt tctcctccgc gaagacgtac gacatcgagg tctgggttcc
      721 ctcgcaggaa cggtacaggg agatctcctc ctgcagcacc ttcaccgact tccaggcgcg
      781 ccgcgcaaag atccgcttcc gggaaggggc ttcggggaag acgcggctgg cccacaccct
      841 gaacgggtcg gggctggcgg tcggtcggac ggtcgtcgcg atcctcgaaa attatcagcg
      901 ggaggacggc accgtcgacg tcccctcgac cctgcgcccc tacatgggcg gactggcacg
      961 gatcacgaaa cgctgttaaa atcgccctat ggaagctcca aagatacggc agatccaccc
     1021 gatgttcgtc cctttcgccg gcgccctgtt cccgacggcc ttcgcggcgt tctgcctgta
     1081 cttcgcgacc ggggcgcggg agttcgaggc gggagcgttc gtctgcgcgc tcttcgggct
     1141 gctggccgcg cccgcgacgg cggcgacggg atttctcgac tggtggacgc attacaaggc
     1201 gtacatgacg agcgtcttcc ggatcaagat cgccggagcg gtcgtcctga tggcgctggc
     1261 ggctcccgcg gtgctgctcc gtgtgttcca ccccgacctt gccgtcctgc cgctgggggg
     1321 gctcggatgg gtatatttcg gccttctcgc cgcctgcacc gccacgtgcg tggtcctcgg
     1381 ctattacggg gggaaactcg tctttcattg atcgaagggt acgaaaccga atacctgcaa
     1441 ggggggaaca ccatgggttc accggaaatg gttgccatcg tgcaccgcgc catcgaccgg
     1501 gagagggacg cgatcaacgt ctacctcggg ctggcgaaga tcgtcacgga cgtgaaggcg
     1561 aagaacgtcc tca
//
LOCUS       DASVJL010000379         3145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_344773, whole genome shotgun sequence.
ACCESSION   DASVJL010000379 DASVJL010000000
VERSION     DASVJL010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344773"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VF357_07985"
     CDS             <1..1191
                     /locus_tag="VF357_07985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7524126.1"
                     /translation="RIQRRGKVEQKIPLHVAYVLPGLAIGGTETQVLTLASRLDRARF
                     SPSVVSTAGDGPLRQSLEDAGVPVTVLDYPGFSLHPARALHLLRGSISFFREMTGAFR
                     KHGVSVVHAFLPGGNVLGACGATLARIPVLIVSKRALCDYKHGHTMFSMAEDLANVRA
                     RAILVNSKAVARDVEANERGWRAKIRLIYNGVDIGVRPARLGDLFPELAGEEDRPVVT
                     YVANFHGYKGHEDLVDAAGFVAREIPDILFLMVGRDAGTMAAVQDRISRLGLEKHMLL
                     PGPRTDTAFIFAASTIAAHPSHEEGFPNAVLEAMAAGKAVVAAAVGGTIEAVQDGRTG
                     ILVPPRNPEALAGALLCLLRDPACARRMGEAGKARVREEFSLKKMVRSYENLYEDLTA
                     DWKR"
     gene            1224..2375
                     /locus_tag="VF357_07990"
     CDS             1224..2375
                     /locus_tag="VF357_07990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7524127.1"
                     /translation="MSPLKITYVLPNVESGGTERHVLQLVRRIDPLRFSLSLVTVAGG
                     GSLHGTFAERIPVTVMGDPARGRRFRKSYLEQLVTLGALTRLLRERKPDILHAYLPAA
                     NVLGPVAARLAGVRRTIVSKRAMADYKALFPLLRRVEPLGNRLADVILVNSDAVRKDV
                     ERTERHWGGKFRKIYNGVAPIEPWAPDEAMAFRRREGIPADALVALCISNFYPYKGHE
                     ELVEAAARIVPAFQNVIFLMVGRDSGTMEATRARVRARGIEGSVRFVGSRTDVPDLLR
                     ASDLFVHPSREEGFSNAILEAMAAGLPVVACDVGGNPEAVTDGETGRLVPSRNAAAFA
                     SAVAELLADPEKRKAMGEAGRHRATERFSLDRMVGEMESLYESLARGER"
     gene            2378..>3145
                     /locus_tag="VF357_07995"
     CDS             2378..>3145
                     /locus_tag="VF357_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524128.1"
                     /translation="MCGIAGFLSTGAARPGERVLRAMGDALAHRGPDASGTHLSPDGR
                     VGLSHRRLSILDLSPAGAQPMFSADGSLVLSFNGEVYNFREIRSELEGKGHAFRGGSD
                     TEVMLASFLEWGVDVAVRRFIGMFAFSLWDARARKLYLVRDRLGIKPLYLARLPGVLL
                     FASELKGLLAYPDFPREVDRAALQYYLEFQYVPGPHAIYRNVEKVLPGRIVEIGQHGE
                     TTDRLYWDLFDHWGNTEGRTRTEGEYEEELSDLLDSSI"
BASE COUNT          530 a          982 c         1099 g          534 t
ORIGIN      
        1 cggatccagc gccgcggaaa ggtggagcag aagatcccgc tacacgtcgc ctacgtactg
       61 cccggcctcg cgatcggagg aacggagaca caggtactga ccctcgcctc ccgcctcgac
      121 cgtgcgcggt tctctccttc cgtcgtgagc acggcgggcg acggcccgct gcggcagtct
      181 ctcgaagacg cgggcgtgcc ggtgaccgtt ctcgactacc ccggcttcag cctccacccg
      241 gcgcgggcgt tacacctgtt gcgaggatcc atctccttct tccgggaaat gacgggtgcg
      301 ttccggaaac acggggtgtc cgtcgtgcac gcattcctcc ccgggggcaa cgtactgggc
      361 gcctgcggcg ccacactggc ccggatcccg gtgctcatcg tctccaagcg cgcgctatgc
      421 gactacaagc atgggcatac tatgttctcc atggcagagg acctggcgaa cgtccgggca
      481 cgggcaatcc ttgtcaattc gaaggcggta gctcgggacg tggaagcgaa tgagcgaggc
      541 tggcgggcga agatccgcct gatatacaac ggtgtcgaca taggggtacg ccctgcgcgc
      601 ctgggggatc ttttcccgga attggccggc gaggaggatc gcccggtggt cacgtacgtg
      661 gccaactttc acgggtacaa gggacacgag gatctcgtgg atgcggcagg gttcgtcgcc
      721 cgggaaatcc ccgatattct attcctgatg gtagggcgcg acgcggggac gatggcggcg
      781 gtgcaggacc ggatctcgcg ccttggcttg gagaagcaca tgctcctccc gggtccaagg
      841 acagatacgg cgttcatttt cgccgcctcc accatcgccg ctcacccgtc ccacgaggag
      901 ggtttcccca atgccgtcct tgaggccatg gccgccggaa aagccgtcgt agcggccgca
      961 gttggcggca cgatcgaggc ggtgcaggac gggcggacgg ggatcctcgt tccaccccgc
     1021 aacccggaag cactcgccgg agcgcttctt tgccttctgc gggaccccgc atgtgcccgg
     1081 cggatgggcg aggccggaaa ggcacgtgtc cgggaggagt tctccctgaa gaaaatggtc
     1141 cggagttatg aaaacctcta cgaagacttg acggcggact ggaaaagatg aacccccact
     1201 cgggtatgat cgaatcatcg gcaatgtcgc ccctcaagat cacatacgta ctgccgaacg
     1261 tcgaatccgg cgggaccgaa cgacacgtac tccaactggt gcggaggatc gaccccttgc
     1321 gtttctccct ttccctcgtg accgtcgctg ggggcggttc gttgcacggg acgttcgcgg
     1381 agaggatccc ggtaacggtc atgggggacc cggcgagggg acggcggttc cggaagagct
     1441 accttgagca actggtgacg ctgggcgccc tgacgcgcct tctccgggag cggaagccgg
     1501 acatcctcca cgcctacctt cctgctgcga acgtcctcgg cccggttgcg gcccgcctcg
     1561 ccggagtgcg acggaccatc gtgagcaagc gggcgatggc ggactacaag gcattgttcc
     1621 cgctgcttcg gcgggtcgag ccgctgggga accgcctggc cgacgtcatc ctcgtgaatt
     1681 cggacgcggt gcggaaagac gtggagcgga cggagcggca ctggggggga aagttccgga
     1741 agatctacaa cggcgtcgcg ccgatcgaac cgtgggcgcc ggacgaggca atggcgttcc
     1801 ggcggcgtga ggggatcccc gccgatgccc tcgtcgccct ttgcatatcc aacttctacc
     1861 cgtacaaggg gcacgaggaa ctggtggagg ccgctgcccg gatcgtgccg gcgttccaga
     1921 acgtcatctt cctgatggtt ggaagagatt ccgggacgat ggaggcgacg agagctcgtg
     1981 tccgggcacg ggggatcgag ggatcggtcc gatttgtcgg tagccggacg gacgtgccgg
     2041 acctcctacg ggcctcggat ctcttcgtcc acccttcccg agaggaggga ttctccaacg
     2101 ccatcctcga agcgatggcg gcgggactgc ccgtggtggc gtgcgacgtc ggaggaaacc
     2161 cagaggcggt aaccgacggg gagacggggc ggctagtgcc gtcccggaac gcggcggcgt
     2221 tcgcctccgc ggttgcggag ttgctcgccg atccggagaa acggaaggcc atgggcgagg
     2281 cggggaggca tcgcgccacc gagcggttct cgctcgatcg gatggtggga gaaatggaat
     2341 ctctctacga gtcgctggcg cggggagagc ggtagccgtg tgcgggatcg ccggcttcct
     2401 ttccaccggg gccgccaggc cgggcgagag agtgctgcgc gcgatggggg atgcgttggc
     2461 gcaccgcggg ccggacgcct ccgggacgca tctctctccc gacggccggg tggggctttc
     2521 ccaccggcgg ctgtcgatcc tcgacctctc ccccgcgggg gcgcagccga tgttctccgc
     2581 ggacgggtcg ctcgtcctct ccttcaacgg ggaggtgtac aacttccgcg agatccgctc
     2641 ggaactcgag gggaagggac acgcgttccg cgggggatcg gacacggagg tgatgctggc
     2701 gtcgttcctc gagtggggag tggacgtcgc ggtccgccga ttcatcggca tgttcgcctt
     2761 ctcactgtgg gatgcgaggg cgcggaagct gtacctcgtc cgcgaccggc tcgggatcaa
     2821 gcccctctac ctcgcccgcc tgccgggggt gctgctcttc gcatccgagc tgaaagggct
     2881 cctcgcgtac ccggacttcc cgcgggaggt ggaccgggcg gcgttgcaat actacctcga
     2941 gttccaatac gtccccgggc cgcacgcgat ctaccggaac gtggagaagg tgctccccgg
     3001 gcgcatcgtg gagatcgggc aacacgggga gaccacggac cgcctctact gggacctgtt
     3061 cgatcactgg gggaacacgg aaggacgcac gaggaccgag ggggaatacg aggaggagct
     3121 gtccgatctg ctcgattcct ccatc
//
LOCUS       DASVJL010000380         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_344954, whole genome shotgun sequence.
ACCESSION   DASVJL010000380 DASVJL010000000
VERSION     DASVJL010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344954"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..733)
                     /locus_tag="VF357_08000"
     CDS             complement(71..733)
                     /locus_tag="VF357_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HEX7524129.1"
                     /translation="MFVARRMKKKIVTVAPSVSLFEAQRKMRDHTIRQLPVISEDGTL
                     LGIVSDRDIRASVLPAGLVPGFTAGEAEKFMKSTPVERVMTRKVVTAMLTDTLEDTIV
                     LLRDFRVNALPVVDSTGKIAGIITRTDVLEAFIDALGVGEVSSRLEVVVPDRPGTLAQ
                     LAAIIGSFHVNITSVLSIQHVEAGKRANFFRVATLNVGPIRKAIEDAGFEILDPSKFL
                     LP"
     gene            complement(758..1708)
                     /locus_tag="VF357_08005"
     CDS             complement(758..1708)
                     /locus_tag="VF357_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX7524130.1"
                     /translation="MKRVAWIYHPDYLLHTPPFEHPESPERLVAITDHLGRVGLIERM
                     VPVTPEYPEDADILSVHDREYLKKLELACRRGDLTLDSEDTYLNRHSFNIALLAAGGA
                     IAGAKAVAEGTADRAFCAVRPPGHHAGRDVGMGFCLLNNAAVAARYLQARHGVGKVLI
                     VDWDVHHGNGTQNIFLEDPTVFYFSIHEHPTFLYPGTGRRWEIGKGKGEGATLNAPMA
                     PGAGDREFRTTFEQTLEPAVERFRPEIILVSAGFDAHRDDPLADLLVTEEGFRFMTRH
                     VLALADRFCGGKVVSILEGGYESSSLTSCIEIHIRELLGE"
     gene            complement(1749..>2105)
                     /locus_tag="VF357_08010"
     CDS             complement(1749..>2105)
                     /locus_tag="VF357_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7524131.1"
                     /translation="NLFAFAPAVICVVGAPYESATDKVLREKDPERYKARRFQVNPGL
                     QGIAAGITQFILAACALGYGTCWMTGPLIAKQELESALSVRHPEELLAIIAVGKPDAA
                     PPKPPRKPASEITTFR"
BASE COUNT          343 a          690 c          709 g          363 t
ORIGIN      
        1 ggatcgggga cgtttcgcag ggagagcggg gaagtcccga tcggcgcgat ccggtcgagg
       61 accgccgccc tcacggaagg aggaacttcg aggggtcgag gatctcgaaa cccgcatcct
      121 cgatcgcctt ccggatcggc ccgacgttca gcgtggcgac gcggaagaag ttcgcccgct
      181 tccctgcttc cacgtgctgg atggagagca cgctcgtgat gttcacgtgg aaggagccga
      241 tgatcgctgc cagttgcgcg agtgttcccg gccggtcggg gacgacgacc tcgaggcggg
      301 aggagacctc gcccacgccg agggcgtcga tgaacgcctc cagcacgtcg gtccgggtga
      361 tgatcccggc gattttcccc gtgctgtcca cgaccggcaa tgcgttcacc cggaagtctc
      421 gcagaaggac gatcgtgtct tccagcgtgt cggtgagcat ggcggtgacg accttgcggg
      481 tcatgacccg ctccacgggg gtgctcttca tgaacttctc tgcctccccg gccgtgaatc
      541 ccggcaccag gcccgcgggg agcaccgagg cccggatgtc ccggtcggac acaatcccca
      601 gcagggtgcc gtcctcagag atcaccggca actgacggat ggtgtgatcc cgcatcttgc
      661 gctgcgcctc gaagagggac acggacgggg ccaccgtcac gatctttttc ttcattctgc
      721 gtgcgacgaa cattgaacca ccctccgtaa gccgggatca ctcgccgagg agttcccgga
      781 tgtgaatttc gatgcaggac gtgagcgagg aggattcata ccctccctcg aggatcgaga
      841 cgaccttccc gccgcagaac cggtcggcca gcgcgagcac gtggcgggtc atgaagcgga
      901 acccctcctc cgtcaccagg aggtccgcca gcgggtcgtc ccggtgggcg tcgaacccgg
      961 ccgagacgag gatgatctcc ggccggaacc gctccacggc cggctcgagc gtctgttcga
     1021 aggtcgtccg gaactcccgg tcccccgcgc cgggggccat cggggcgttg agcgtcgccc
     1081 cttccccttt ccccttgccg atctcccacc ggcggccggt gccggggtag aggaaggtgg
     1141 ggtgctcgtg gatgctgaaa tagaagaccg tcgggtcttc gaggaagatg ttctgcgtgc
     1201 cgttgccgtg gtggacgtcc cagtcgacga tgagaacctt ccccacaccg tgcctcgcct
     1261 ggaggtaccg ggccgcgacg gccgcattgt tgaggaggca gaaccccatc cccacgtccc
     1321 gtcccgcgtg gtgccccgga ggccggacgg cgcagaaggc gcggtcggcc gttccttccg
     1381 ccaccgcctt cgcccccgcg atcgcccccc ccgccgccag gagcgcgatg ttgaaggagt
     1441 gccggttgag gtaggtgtcc tccgagtcga gggtgaggtc gccgcgccgg caggcgagtt
     1501 ccagcttctt cagatattcc cggtcgtgga cggaaaggat atccgcgtct tccggatact
     1561 ccggcgtcac cgggaccatc cgttcgatca ggccgactct cccgagatga tcggtgatgg
     1621 cgacgaggcg ctccggagac tccgggtgtt cgaaaggcgg ggtgtggagg aggtagtcgg
     1681 ggtggtagat ccaggccact cgcttcatga gcgctccata cacaccccga cactatatca
     1741 attaccggct accggaacgt cgtgatctcc gacgccggtt tgcgcggtgg tttcgggggc
     1801 gccgcgtccg gtttccccac ggcgatgatc gccagcagct cttcgggatg ccggacggag
     1861 agtgccgatt cgagctcctg cttcgcgatc agggggccag tcatccagca ggtgccgtac
     1921 cccagcgcgc aggccgccag gatgaactgg gtgatcccgg ccgcaatccc ctgaagcccc
     1981 gggttcacct ggaaacggcg cgccttgtac cgctcggggt ccttctcgcg gagcaccttg
     2041 tccgtcgccg attcgtaggg ggcgccgacc acgcagatca cggcgggggc gaacgcgaac
     2101 aggtt
//
LOCUS       DASVJL010000381         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1565652, whole genome shotgun sequence.
ACCESSION   DASVJL010000381 DASVJL010000000
VERSION     DASVJL010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565652"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VF357_08015"
     CDS             <1..764
                     /locus_tag="VF357_08015"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7524132.1"
                     /translation="SFAIPEYTGDLTGLGTTRILESLRRAGGKARYYQASSSEMFGAT
                     PPPQGENTPFYPRSPYGAAKVYSYWMAINYREAYNMFASNGILFNHESPRRGETFVTR
                     KITRAIAKIKAGKQKELFLGNLDARRDWGFAYEYVQAMWRILQHDRGDDFVIGTGQSN
                     SVKEFLEEAFAYADLDWKEYVKIDRKYFRPTEVVNLIADASKARKLLGWEPKVTFKEL
                     VRIMVDADLEDAGLPTPGEGRKILAAKGLPVGSKL"
     gene            842..1813
                     /locus_tag="VF357_08020"
     CDS             842..1813
                     /locus_tag="VF357_08020"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7524133.1"
                     /translation="MSGFAGKKILVTGGSGFLGAPVIAQLLGRGARQEDITVPRSARY
                     DLRLPAACAEVAAGQDLIIHLAANAGGIGWNRAHPGSLFYDNAAMGIHLMEESRKAGV
                     KKFVQIGTVCAYPFQPPHIPFREDDLWMGYPERTNAPYGMAKKMMMVMGQAYRQEYDF
                     NVIYLLPVNLYGPRDHFFEPEKSHVIPALIQKFVDARDSGAPEVIVWGSGYFKGTPVS
                     REFLYVDDAAEAIALAAERYEGAEPVNIGAGHEVPINDLIAMVCEMTGYRGKVVRDLS
                     KPDGQPRRCLATSRATEKFGFVARTPFEEGLRKTIAWYEQARRERNG"
BASE COUNT          351 a          637 c          630 g          290 t
ORIGIN      
        1 tttccttcgc catccccgaa tacaccggcg acctcaccgg cctcggcacg acccgcatcc
       61 tcgagtcgct gcgccgcgcg ggcggcaagg cccgctacta ccaggcctcc tccagcgaga
      121 tgttcggcgc cacccccccg ccgcagggcg agaatacgcc cttctatcca cgctccccat
      181 atggggccgc caaggtgtac tcctactgga tggcgatcaa ctaccgggag gcgtacaaca
      241 tgttcgccag caacgggatc ctcttcaacc acgagtcccc ccgccgcggc gagacattcg
      301 tcacccgcaa gatcacccgc gcgatcgcga agatcaaggc cgggaagcag aaggagctgt
      361 tcctcggcaa cctcgacgcc cggcgcgact gggggttcgc ctacgaatac gtccaggcca
      421 tgtggcgcat cctgcagcac gaccggggcg acgatttcgt catcggcacc ggccagtcga
      481 actcggtgaa ggagttcctc gaggaggcgt tcgcctacgc ggacctcgac tggaaggagt
      541 acgtgaagat cgaccggaaa tatttccggc cgacggaagt ggtgaacctg atcgccgacg
      601 cttcgaaggc gaggaagctg ctcggctggg agccgaaggt gacgttcaag gagctggtgc
      661 ggatcatggt cgacgccgac ctcgaggacg cgggcctccc gacccccggc gagggccgca
      721 aaatcctcgc cgccaagggc ctccccgtcg gctcgaaact ctaacccgtt gaacagggac
      781 gttcctgcga actcccgttg aacagggaca ctcctgtgtg tcgagatatc atggagccgg
      841 gatgagcgga ttcgcgggaa agaagatcct ggtcaccggc gggtcggggt tcctcggggc
      901 tcccgtaatc gcgcaactgc tcggtcgcgg cgcgcggcag gaggacatca cggtcccgcg
      961 gtcggcgcgg tacgaccttc gcctcccggc ggcttgcgcg gaggtcgccg cggggcagga
     1021 cctcatcatc cacctcgcgg ccaacgcggg cgggatcggc tggaaccggg cgcaccccgg
     1081 ctcccttttc tacgacaacg ccgcgatggg gatccacctg atggaggagt cccgcaaggc
     1141 cggcgtgaaa aaattcgtcc agatcggcac cgtctgcgcc tacccgttcc agccgccgca
     1201 catccccttc cgcgaggacg acctctggat ggggtatccc gaacggacca acgccccgta
     1261 cggcatggcc aagaaaatga tgatggtgat ggggcaggcg taccggcagg agtacgattt
     1321 caacgtgatc tacctgctcc ccgtgaacct gtacggcccc cgcgaccact tcttcgagcc
     1381 ggagaagtcg cacgtcatcc cggcgctgat ccagaagttc gtcgatgccc gcgactccgg
     1441 cgcgccggag gtgatcgttt gggggagcgg ctacttcaag ggcacccccg tatcccgcga
     1501 gttcctctac gtggacgacg cggccgaggc gatcgcgctg gcggccgagc ggtacgaagg
     1561 ggcggaaccg gtgaatatcg gcgcgggaca cgaggtgccg atcaacgacc tgatcgccat
     1621 ggtgtgcgag atgaccggct accgggggaa ggtggtccgc gacctctcga agcccgacgg
     1681 ccagccccgc cgctgcctcg ccacctcccg cgcgacggag aagttcggct tcgtcgcccg
     1741 cacgccgttc gaggaaggcc tgcgcaagac gatcgcgtgg tacgagcagg cgcgacggga
     1801 acggaacggt tgaaaatccg gtggagcgag ggggagggat gaagcgcgtc ctggtcaccg
     1861 gcggcgccgg gttcatcgga tcgcacctgt gcgagcggct gctggcgc
//
LOCUS       DASVJL010000382        11910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_345169, whole genome shotgun sequence.
ACCESSION   DASVJL010000382 DASVJL010000000
VERSION     DASVJL010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 11910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11910
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345169"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="VF357_08025"
     CDS             complement(<1..1448)
                     /locus_tag="VF357_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX7524134.1"
                     /translation="MRKHMLVVDLSSGRMRREEIPPEFLRAYLGGRGLGVRLLRGRHT
                     LSPFDPAMPVVFAVGPLCGTPAPTSARLSVVSRSPLTGTVYDCSAGGRFAWRLRAAGL
                     LAILVEGESPRPVVLAVTSSGEELLPAEKLWTKGVGETAAALSDRGSVAAIGPAGENR
                     VLFSSIMMGEGNAVGRGGLGAVLGKKNLKAVTVDGDRTVPVADRERFDRARADVLRLF
                     RASPVIFGELGISEYGTPALVDLMRQRRMAPTENFRHTVFDGAEAYSGPTIRAAYGAK
                     KEGCFGCPIRCKKRASDGRPLPEYETVSHFGALNAISDLHAIVRSNDACNDLGMDTIS
                     AAATLSAWGEARGRFPSPGEIGPLLHDIAHRRGEGDLLAEGSRRAAEALGKPEVSMSV
                     KSLELPAYDPRGAYGMALAYVTSNRGGCHLRAYPISHEILRKPVASDRFSFSGKARMI
                     KIAEDANAAVDSLVACKFAFFGASLEEYAELLS"
     gene            complement(1653..2876)
                     /locus_tag="VF357_08030"
     CDS             complement(1653..2876)
                     /locus_tag="VF357_08030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7524135.1"
                     /translation="MSALLNAFGNRRVFFVLLLGFSSGIPLALTGSTLQAWMASEKVD
                     LTVIGIFSLVGLPYTFKYLWSPLMDRFVPPFLGRRRGWMLVTQAALFLAIAAMAYSEP
                     KAHPGALALLSFLVAFCSASQDIVVDAWRTEILAPEELGPGAGVHILGYRVAMLTSGA
                     IALILADRMPWRVVYLLMAGSLVVGIVASLLAPEPELAAKRPRTLKEAVVEPFLEFFS
                     RRGAAGILLFIVFYKLDVVMATALTTPFLLELGFTKTDIGAVTKGLGMIATIAGTLAG
                     GAIVAGTGMKASLWIFGILQSVSTLSFLALARLGHHYPMMVAAIGIENLCSGMGTAAY
                     AAFLMSLCDKRFTATQYALLTSLMAVTRVVVGAPTGFLAKTYGWESYFIISTLAAIPG
                     LLFLLRYDRWKTSSA"
     gene            2934..3371
                     /locus_tag="VF357_08035"
     CDS             2934..3371
                     /locus_tag="VF357_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524136.1"
                     /translation="MGFDAQGFLDRYARAYNGRDPEGMRTFFALSDERFAVFEDFTGD
                     LIDGGAYSAMLEAAFDATGRMSFELLRCDDFGGIAVIHAIQRIVLEDEEEGIGEARIR
                     ATLWVKTGEDVPRVVSAHFSAVPTAVEECGMGGCGCAGHAGDD"
     gene            3371..4621
                     /locus_tag="VF357_08040"
     CDS             3371..4621
                     /locus_tag="VF357_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX7524137.1"
                     /translation="MGVVAVILLVLVALAAGVAAGWFLRAGQVATLAERLQGQERLMD
                     EKLSVLNEARGKFVESFQALSAEALRGNNQSFLTLAKSTLETFQEGARGDLEMRQRSI
                     DALILPMRDSLQRVDSKIGELEKARFAADATLAEQIRSLIETQGRLQSETANLVKALR
                     APTVRGRWGEIQLKRVVEIAGMLAHCDFVEQESVTTEDGRLRPDMIVRLPNHKNIVVD
                     AKAPLSAYLEALEAPDEGTRTARLKDHARQIRAHLSALASKGYWDQFAPAPEFAVLFL
                     PGETFFSAALEQDPGLIEFGAGERVILATPTTLIALLKAVAYGWRQEQVAENAQEISR
                     LGKEMYERIGKFTEHFTRIRDGLDKAVSAYNSAVGSYESRVLPGARRLRELRVTSDRE
                     GMKPPEIVDRATRPAPEGEDAPPS"
     gene            complement(4637..5242)
                     /locus_tag="VF357_08045"
     CDS             complement(4637..5242)
                     /locus_tag="VF357_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX7524138.1"
                     /translation="MIRGVIFDFGNVICSFDVGKFLAKLHEWSGLDVETLRDRVYGSR
                     LHSRYERGEISSKEFHREVVRRIGVDVPVEELADRFTDIFTPLESTHGLIRSLKGKYR
                     LGLLSNTNEWHFLRHIRKVPVFPLFDAVTLSFEVGALKPEPEIYLDALRKLSLPPEAC
                     VFIDDIGKYAEGAAALGIRGIRYTGHAELLRELSGLGVVPP"
     gene            complement(5239..6852)
                     /locus_tag="VF357_08050"
     CDS             complement(5239..6852)
                     /locus_tag="VF357_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HEX7524139.1"
                     /translation="MGNIGDYGETRKNFRWNVPDDFNFGRDVVDRYANDRTKLAFFYE
                     DANGNKSKYTFWEVSRISNRVGNVLRSLGVKKGDAMLVLLQNVPEWYFTVVAGNKIGA
                     IVIPCSDQLRPKDLVYRALHSGASTIVSWDAKTAEVDEIRKECPALVNFLCVGRKQDG
                     WRSFDEETSNASPELEVEPCRANEPALVYYTSGTTGNPKAVMHSHFYTGAHWVTGKYW
                     IDLKPTDLLWCIAGTGWAKAAWSVLFGPWNNGAATLLLNAPFDAKRCLSWLAGYEVTV
                     LCAPPTAYRAFVKEDLGKHRLPSLRHCVGAGEPLNPEVIRMWKDAFGITIHDGYGQTE
                     TINLVANFPGIPVKPGSMGLPVPGHDVAVIDEKGNEAPAGEIGEIAVKGRPPALFMGY
                     WKDENKTKECFRGDWYLTGDRAYRDGDGYFFFVGRGDDVIISSGYRIGPFEVESALLE
                     HPAVVESAVVSSPDELRGEIVKAYVVLRAGYEPGDPLKKELQEHVKKVTAPYKYPRDI
                     AFVTDLPKTTSGKIRRIELRAKEWKKGVV"
     gene            7003..8247
                     /locus_tag="VF357_08055"
     CDS             7003..8247
                     /locus_tag="VF357_08055"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX7524140.1"
                     /translation="MRFRTWVCIAAGSGFLLLAGCAANRAAMTGSAEMPYPSSVPPKV
                     EEIFHLPTGLRLSIEGMIEMLSGARLVSVGETHDNLNDQRVELTVVRELHRRFPGKVA
                     VGMEMFREPQQAVLDQWVRGELTELEFLKAAKWYETWGFDFGAYRELLLFAKENRIDV
                     IALNPPKELQEAVRRTGLDNVPEDLRRKLPEIGETDPWQRAVLRGVFGGHAGHGGGDE
                     SFDSFLRVQLLWEETMAQKVVDYLKSPRGEGKRMVTITGGWHVKYGFGLPKKVVRRLP
                     MAYAIVLPEEISTPEQKEGRLMRIELPEVPLLPAHFAWYVPYNNIEEKRVRMGIRMEE
                     KEGRLLVESVAPGSPAEKAGIAKGDELLALDGQPVKDTVDVFFRVGEKSQGDMAQVTV
                     RRGGEVKTLPITFFRMPKPKAH"
     gene            8337..8675
                     /locus_tag="VF357_08060"
     CDS             8337..8675
                     /locus_tag="VF357_08060"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7524141.1"
                     /translation="MPFFIRMIANAVAILLIGYLLPQIVTVDGVMAALAAAFVLGLVN
                     AVVRPLFVLLTLPVTVVTLGAFLLVINGLLLLLVSAFVPGFHVNGFLAAVAGSVLLSV
                     VSWILTRVVQ"
     gene            8672..10072
                     /locus_tag="VF357_08065"
     CDS             8672..10072
                     /locus_tag="VF357_08065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7524142.1"
                     /translation="MSVTLTFLGAAREVTGSCILVQTARNRFLVDCGMFQGGGESDQK
                     NARRMPVPPASIDFVLSTHAHIDHSGLLPKLVRDGFRGPIHCTSATADLLGVMLPDAG
                     HIHEKEAEWQTRKRQRGGKREVPPLYTEADAMAVLPALRPVPYGESIVPGPGVSAAFL
                     DAGHILGSAIVTVTVADEGREKKLVFSGDLGHRGLPIVRDPAPVPRGDVLVIESTYGN
                     RVHKGMEETIEEFVRAVDDTLHRKKGNVVIPSFAVGRAQDILYLLTDLTRKGRLSGIT
                     LYIDSPLAAEATRITMRHPECYDDETRELFAWRDAHPDALEVVLVRNMEESRELNSLR
                     GGGILMAGSGMCDAGRIKHHLKHNLWRKECSVIIVGFQAQGTLGRKIVDGAKRVRVFG
                     EEIAVAADVYTIGGLSAHADRDDLLAWAGKFQAPPGNVFVAHGEESVSLEFAGTLKEK
                     LGWLAQVPSPGQPLIV"
     gene            10081..10911
                     /locus_tag="VF357_08070"
     CDS             10081..10911
                     /locus_tag="VF357_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7524143.1"
                     /translation="MMRSILRHVLLCVLVALFPGAAHAADPQDDILRQIGVDEKPGAQ
                     VPLDLPFTDASGRTVRLGDYLRDGPVLLTLNYYTCPMLCPLTFRGLAATMGEVKDLSP
                     SKDYRIVTVSIDPAEIPEIVRAKANESHALVPGLKDADSRWPFLYGDGETIRRVTEAV
                     GYRYKKVGAEFAHPSVQIVLSPGGKVSRYLYGFEIAPRDFKLALLEASEGKIGASTAG
                     NAILMYCFRYDPVGKKYMLYARNIMKAAGAVTLVLLAGLLVVLWRKYGRTPGPPGKGE
                     "
     gene            10911..11861
                     /gene="coxB"
                     /locus_tag="VF357_08075"
     CDS             10911..11861
                     /gene="coxB"
                     /locus_tag="VF357_08075"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX7524144.1"
                     /translation="MDNVLAFGAASAQAGRVDAVFLMILVIGVFFFFLTQGILIYFAV
                     KYRRRLPGRDNETPAITGNPLLEFLWILIPSIVVVVIFYYGWRVYTDQRIPVAGATEV
                     YVNARQWLYEIKYPDGRTAINEIRVPAGKSVKFILSAADVLHGFYLPDFRVKMDMVPG
                     RVTTLWVQPDRPGSYQIFCTVYCGTGHSNMLARLIVMPPREYAEWVEHGGREGGEGGR
                     HEPLAVRGERILKNAGCSNCHAVDGKQKMAPGFRGLYGSRVPLEGGISVTADEEYLRE
                     SIVDPGAKIVKGFPNVMPTFKTTLSKEDIDAVVAYLKTLK"
BASE COUNT         2056 a         3807 c         4085 g         1962 t
ORIGIN      
        1 gagagaagtt cggcgtactc ctcgagcgac gcgccgaaga aggcgaactt gcacgccacg
       61 agggagtcca ccgccgcgtt cgcgtcctcc gcgatcttta tcatccgcgc cttccccgag
      121 aatgaaaacc ggtccgacgc cacgggcttg cgaaggatct cgtgggagat gggatacgcc
      181 cgcagatggc atccgccccg gttgctggtg acgtaggcca gcgccatccc gtaggcccct
      241 cgcgggtcgt aggcgggcaa ctccagcgac ttgaccgaca ttgagacttc cggtttcccg
      301 agggcctccg ccgcccgccg ggacccctcg gcgagaagat ccccctcgcc cctgcggtgg
      361 gcgatgtcgt ggagaagcgg tccgatctct cccggggaag ggaaccgtcc ccgcgcctcg
      421 ccccacgcgg aaagggtggc ggcggcggag atcgtgtcca tcccgaggtc gttgcaggcg
      481 tcgttcgagc ggacgatcgc gtggagatcg gagatggcgt tcaatgcccc gaagtgcgaa
      541 accgtttcat actccggcaa gggacgtccg tcggacgcgc gcttcttgca acggatgggg
      601 cagccgaaac agccctcctt tttcgcgccg tacgcggcgc ggatcgtcgg accggaatag
      661 gcttcggccc cgtcgaacac ggtatggcgg aagttctccg tgggagccat ccgacgctgg
      721 cgcatgaggt cgaccagcgc gggggtcccg tactcggaga tcccgagctc cccgaagatc
      781 acgggggagg cccggaacag gcgcaggaca tccgcgcggg cgcggtcgaa ccgctccctg
      841 tcggccaccg gcacggtgcg gtcgccgtcc accgtcacgg ccttgaggtt cttctttccc
      901 aggacggcgc cgagaccgcc gcgtcccacc gcattcccct cgcccatcat gatgctggag
      961 aagaggaccc gattttcccc ggcggggccg atggcggcga cgctaccgcg gtccgacagg
     1021 gcggccgccg tctctcccac gcccttcgtc cacaactttt cggccggaag tagttcctct
     1081 ccggaggagg tgacggcgag cacgacaggg cgggggcttt ccccctccac gaggatcgcg
     1141 aggagtcccg cggcgcggag gcgccacgcg aaccggcctc ccgcggagca gtcgtacacg
     1201 gtcccggtca gcggagagcg ggagaccacg gagaggcgcg cggacgtcgg cgccggggtc
     1261 ccgcacaggg ggccgacggc gaagacgacc ggcatcgccg gatcgaacgg ggaaagcgtg
     1321 tggcgccccc ggagcaggcg cacgccgagg ccgcgccccc ccaggtacgc ccggaggaat
     1381 tccgggggga tctcctcccg ccgcatccgc ccggaagaga ggtcgacgac gagcatatgt
     1441 ttgcgcaagg gcatccgcct attgtacccg ctctatggga cccatcgtac gtttgctggc
     1501 gggagtgacg cagcggggga ttccacgcag cggcgtctgg agcgaagtgg agacagggac
     1561 gtcgggaatg agcggaggcg caggcgagga gaccatggat ggtcgacgag ccgtagcgga
     1621 gtggaagccc cccgcgagga accccgccag ctttacgcgg aggaggtttt ccaccggtcg
     1681 taccgcagca ggaagagcag gcccggtatg gcggcgaggg tgctgatgat gaagtagctt
     1741 tcccagccgt acgtcttggc gaggaatccg gtgggcgcgc cgacgaccac gcgcgtgacc
     1801 gccatgaggc tggtgagcag ggcatactgg gtggcggtga accgcttgtc gcacaggctc
     1861 atgaggaagg cggcgtacgc cgccgttccc atcccgctgc acaggttctc gatcccgatc
     1921 gccgccacca tcatcgggta gtgatgcccc aggcgggcca gcgcgaggaa cgagagggtg
     1981 gacaccgact ggaggatccc gaagatccac agggacgcct tcatcccggt ccccgcgacg
     2041 atggcgccgc ccgcgagggt ccccgcgatc gtcgcgatca tccccaaacc cttcgtcacg
     2101 gccccgatgt cggttttcgt gaagccgagc tcgaggagga acggggtcgt cagcgcggtc
     2161 gccatcacaa cgtcgagctt gtagaagacg atgaacagca ggatcccggc cgcgccgcgc
     2221 cgggagaaaa actccaggaa cggctcgacg accgcctcct tcaacgttct cggcctcttc
     2281 gcggccagtt ccggttccgg ggcgagaagg gaggcgacga tccccacgac gagggagccg
     2341 gccatcagga gatagacgac gcgccacggc atccggtcgg cgaggatcag ggcgatcgcg
     2401 cccgaggtga gcatcgccac gcggtatccg aggatgtgaa cccccgctcc cggtcccagc
     2461 tcctccgggg cgagaatctc ggtgcgccag gcgtcgacca cgatgtcctg gctcgcgctg
     2521 cagaaggcga cgaggaacga caggagcgcc agcgcgccgg gatgcgcctt cggctcggag
     2581 tacgccatcg cggcgatggc gaggaagagg gccgcctggg tcaccagcat ccacccgcgg
     2641 cgacgcccga ggaacggggg gacgaaccgg tccatcagcg gggaccagag gtacttgaac
     2701 gtgtacggca ggccgacgag ggagaagatc ccgatcaccg tcaagtcgac cttttcggac
     2761 gccatccacg cctgcagagt cgagcccgtc agcgcgaggg ggatcccgga ggagaaaccg
     2821 agcagcagga cgaaaaagac gcgccggttc ccgaaagcgt tcagaagcgc ggacatgcgg
     2881 ggtatcgtac cactctgcta tgatgacggt acttccatgt aaaggggtgg agcatggggt
     2941 ttgacgcgca aggttttctg gacaggtacg cgagggcgta caatggccgg gacccggagg
     3001 ggatgcggac cttcttcgcc ctgtccgacg agcggttcgc ggtcttcgag gatttcaccg
     3061 gggacctgat cgacggcggc gcgtactcgg cgatgctcga ggcggcgttc gacgccacgg
     3121 gccggatgtc cttcgagctt ctgcgctgcg atgatttcgg cgggatcgcc gtcatccacg
     3181 cgatccagag gatcgtgctg gaagacgagg aggaggggat cggcgaggcg cggatccggg
     3241 ccaccctctg ggtcaagacc ggcgaggacg tcccccgggt ggtgtccgcg catttttccg
     3301 ccgtccccac cgccgtcgag gagtgcggca tgggcgggtg cggctgcgca ggacatgcgg
     3361 gggacgattg atgggggttg tcgccgtcat cctcctcgtt ctcgtcgcgc tcgccgcggg
     3421 agtcgccgcg ggctggttcc tccgcgcggg acaggtcgcg acgctcgcgg agcggctgca
     3481 ggggcaggag cggctcatgg acgagaagct ctccgtcctg aacgaggcga ggggaaaatt
     3541 cgtcgagtcg ttccaggcgc tatcggcgga ggcgttgcgc ggcaacaacc agtcgttcct
     3601 gacgcttgcg aaatccacgc tcgagacgtt ccaggagggg gcgcgtggtg atctcgagat
     3661 gcgccagcgg tcgatcgacg cgctgatcct gccgatgcgc gactcccttc aaagggtgga
     3721 ctcgaagatc ggggagctcg agaaggcgcg gttcgccgcc gacgccacgc tggcggagca
     3781 gatccgttca ctgatcgaga cgcaggggcg cctccagtcg gagacggcga acctggtgaa
     3841 ggcgctgcgg gcgccgacgg tccgcggtcg ctggggggag atccagctca agcgcgttgt
     3901 cgagatcgcg gggatgctcg cgcactgcga cttcgtcgag caggagagcg tgaccaccga
     3961 ggacgggcgc ctgcgccccg acatgatcgt gcggcttccc aaccacaaga acatcgtggt
     4021 ggacgccaag gcccccctct ccgcgtacct cgaggccctc gaggcgccgg acgaggggac
     4081 ccggaccgcg cggctcaagg accacgcccg gcagatccgc gcccatcttt ccgccctggc
     4141 ctccaaggga tattgggacc agttcgcccc cgcccccgaa ttcgccgtcc tgttcctccc
     4201 gggcgagacg ttcttcagcg cggcgctgga gcaggatccg gggctcatcg aattcggcgc
     4261 cggcgagcgg gtgatcctcg ccacccccac gacgctcatc gcgctgctga aggccgtcgc
     4321 ctacggctgg cgccaggagc aggtcgcgga gaacgctcag gagatcagcc ggctgggaaa
     4381 ggagatgtac gaacggatcg ggaagttcac ggaacatttc acgcgcatcc gcgacgggct
     4441 ggacaaggcg gtctccgcgt acaacagcgc cgtggggtcg tacgagtcgc gcgtcctccc
     4501 gggggcgcgc cggctgcggg agctccgggt cacgtcggac cgcgagggca tgaagccgcc
     4561 ggaaatcgtc gaccgcgcca cccggccggc ccccgagggc gaggacgccc ccccctcctg
     4621 atcaggggga cgatctttac ggcgggacga caccgagacc ggagagctcc cggagcagct
     4681 ccgcgtggcc ggtataccgg atcccccgga ttccgagcgc ggcggcgcct tccgcgtact
     4741 tcccgatgtc gtcgatgaaa acgcacgcct ccggcgggag ggagagcttg cgcagggcgt
     4801 cgaggtagat ctccggctcc ggcttgaggg cgccgacctc gaacgacagg gtgacggcgt
     4861 cgaagagggg gaagacgggc accttgcgga tgtgccgcag aaagtgccac tcgttggtgt
     4921 tggagagcag gccgagccgg tactttccct tgagcgaccg gatcaacccg tgtgtggact
     4981 cgaggggggt gaagatgtcg gtgaaccgat ccgcgagctc ctcgaccgga acgtccacgc
     5041 cgatacggcg gacgacctcc cggtggaact ccttcgagga gatctcgccg cgctcgtacc
     5101 gggagtggag gcgggatccg tagacccggt cgcgcaacgt ctccacgtcg agccccgacc
     5161 attcatggag cttcgccagg aatttcccca cgtcgaacga acagatgacg ttcccgaagt
     5221 cgaaaatgac cccgcggatc acacgacgcc tttcttccac tccttggcgc gaagctcgat
     5281 ccgccggatc ttccccgagg tggtcttcgg gaggtcggta acgaaggcga tatccctcgg
     5341 gtacttgtac ggtgcggtca cctttttcac gtgctcctgg agttccttct tcagcggatc
     5401 gcccggttcg taccctgcgc gcagaacgac gtaggccttg acgatctccc cccgtagttc
     5461 gtcgggagac gagaccaccg ccgactcgac caccgccggg tgctcgagca gggcgctctc
     5521 gacctcgaaa ggaccgatgc ggtaccccga ggagatgatg acgtcgtccc cgcgccccac
     5581 gaaaaagaag tagccgtcgc catcgcggta tgcccggtcg cccgtcaggt accagtcgcc
     5641 gcggaagcac tctttcgtct tgttctcgtc cttccagtac cccatgaaga gggccggggg
     5701 gcgccccttc accgcgatct ccccgatctc gccggccgga gcctcgttcc ccttctcgtc
     5761 gatcaccgcc acgtcgtgcc ccggcacggg gagccccatg gagcccggct tcacgggtat
     5821 ccccgggaag ttcgcgacca ggttgatcgt ctcggtctgg ccgtacccgt cgtggatggt
     5881 gatcccgaag gcgtccttcc acatcctgat gacctccggg ttcagcggct cgcccgctcc
     5941 aacgcagtgg cgaagagacg gcagccggtg tttgccgagg tcttccttga cgaaggcgcg
     6001 gtaggcggtc ggcggcgcgc aaagcacggt gacctcgtac cccgcgagcc acgaaagaca
     6061 ccgcttcgcg tcgaagggcg cgttcagcag cagcgtcgcc gccccgttgt tccaggggcc
     6121 gaacaggacg ctccacgccg cctttgccca gccggtcccg gcgatgcacc acaggaggtc
     6181 cgtgggtttc aggtcgatcc agtacttccc cgtgacccag tgcgcccccg tgtagaagtg
     6241 gctgtgcatg accgctttcg ggtttcccgt ggtgccggag gtgtagtaca ccagtgccgg
     6301 ctcgttcgcc cggcacggtt ccacctcgag ttcgggggag gcgttcgacg tctcctcgtc
     6361 gaaggaacgc cagccgtcct gcttccgccc cacgcacagg aagttgacga gggccgggca
     6421 ctccttccgg atctcgtcga cctcggcggt cttcgcgtcc cagctgacga tcgtgctcgc
     6481 cccggagtgg agcgcgcggt agacgagatc cttcgggcgg agctggtcgg agcaggggat
     6541 gacgatggcc ccgatcttgt ttcccgccac caccgtgaag taccattcgg ggacgttctg
     6601 gaggagcacc agcatcgcat cccccttctt cacgccgagg gagcgcagga cgttccccac
     6661 gcgattggag atccggctta cctcccagaa cgtatacttc gacttgttcc cgttcgcgtc
     6721 ctcgtagaag aaggcgagct tggtccggtc gttcgcgtac cggtcgacca cgtcccggcc
     6781 gaaattgaaa tcgtccggaa cgttccaccg gaagttcttc ctggtctccc cgtaatcccc
     6841 gatatttccc atcgaacgac ctccatggag taggatgccg atcggggaaa ctacagggat
     6901 cgactggata tgtcaatggg aggatggaac ttccctcccg gattccgtat ctatggatgg
     6961 gtggggaacg acgatgccgt ggagatggaa aaggaggaat cgatgcggtt tcggacatgg
     7021 gtatgcatcg ccgcgggctc ggggtttctg ctcctcgcgg ggtgtgccgc gaaccgcgct
     7081 gcgatgacag ggagcgcgga gatgccgtac ccgtcttccg taccgccgaa ggtggaggag
     7141 atcttccacc ttcccacggg cctgcggctt tccatcgagg ggatgatcga gatgctctcc
     7201 ggtgcccgcc tggtgtcggt gggggagacg cacgacaacc tgaacgacca gcgggtcgag
     7261 ctgaccgtgg ttcgcgagct ccatcgtcgt tttcccggga aggtcgccgt cgggatggag
     7321 atgttccggg agccgcagca ggcggttctc gaccagtggg tcagggggga gttgacggag
     7381 ctcgagttcc tgaaagccgc gaagtggtac gagacgtggg ggttcgactt cggcgcctac
     7441 cgggagctgc tcctcttcgc gaaggagaac cggatcgacg tgatcgccct gaatccgccg
     7501 aaggaattgc aggaagcggt ccgccggacg gggctggaca acgtgccgga ggaccttcgc
     7561 cggaagctcc ccgagatcgg cgagaccgat ccgtggcagc gcgccgtcct gcgcggcgtc
     7621 ttcggggggc atgcggggca cggcggcggg gacgaatcgt tcgactcctt cctccgcgtc
     7681 caactgctct gggaggagac gatggcgcag aaggtggtcg attacctgaa gagcccccgg
     7741 ggggagggga agcggatggt gacgatcacc ggcggctggc acgtgaaata cggcttcggg
     7801 ctcccgaaga aggtggtgcg ccgcctgccg atggcgtacg cgatcgtcct ccccgaggag
     7861 atcagcacgc cggagcagaa ggaggggcgg ctgatgcgga tcgaactgcc cgaggtcccg
     7921 ctgctccctg cccatttcgc atggtatgta ccgtataaca acatcgagga gaagcgggtc
     7981 cgcatgggga tccggatgga ggagaaggag gggcgcctcc tcgtcgaatc cgtcgcgccg
     8041 gggtctcccg cggagaaagc cgggatcgca aagggggacg aactcctcgc gctcgacggg
     8101 cagccggtga aggataccgt cgacgtcttc ttccgggtcg gcgagaagag ccaaggggac
     8161 atggcgcagg tcaccgtccg gcgcggcggg gaagtgaaga cgctgccgat caccttcttc
     8221 cggatgccga aaccgaaagc gcactgagcg gtcgatgtcc gccatagggc agccccccgc
     8281 gaggaactct ccgactcggg gtatacttca atgagtccag cggaagggag ggcatcatgc
     8341 cgttcttcat ccggatgatc gcaaacgccg tcgcgatcct gttgatcggg tacctgctgc
     8401 cccagatcgt caccgtggat ggagtgatgg cggcgctggc cgccgccttc gtgctggggc
     8461 tcgtgaacgc ggtcgtccgg cccctcttcg tgctgctgac ccttcccgtc acggtggtga
     8521 cgctgggggc cttcctcctg gtgatcaacg ggctgctcct gttgcttgtc tccgccttcg
     8581 tcccggggtt ccacgtgaat ggcttcctcg cggcggtggc cggctccgtc ctcctctccg
     8641 tcgtctcatg gatcctcacg agggtggtcc aatgagcgtc accctgacgt ttctgggcgc
     8701 cgcgcgggaa gtcaccggct cctgcatcct cgtgcagacg gcgcgcaacc ggttcctcgt
     8761 cgattgcggg atgttccagg ggggagggga gagcgaccag aagaacgcac gccggatgcc
     8821 cgtccccccc gcgtcgatcg acttcgtcct ctcgacccac gcccacatcg accactccgg
     8881 cctgcttccg aaactcgtgc gggacggttt tcgcggtccc atccattgca cttccgccac
     8941 cgccgacctc ctcggggtga tgcttcccga cgccggccac atccatgaga aggaggcgga
     9001 gtggcaaacc cggaagcggc agcggggagg aaagcgggag gtccccccgc tctacaccga
     9061 ggccgacgcg atggccgtcc ttcccgcgct gcgccccgtt ccctacgggg aatcgatcgt
     9121 ccccgggccg ggggtctccg ccgccttcct tgacgcgggg cacatcctcg gctcggcgat
     9181 cgttaccgtg accgtcgcgg acgagggaag ggaaaagaag ctggtcttct ccggcgacct
     9241 cggccaccgg gggttgccga tcgttcgcga tcccgccccc gtcccgcgag gcgacgtcct
     9301 cgtcatcgaa tcgacgtacg gcaaccgggt ccacaagggg atggaggaga cgatagagga
     9361 gttcgtccgc gccgtcgacg acacgctcca ccggaagaag gggaacgtcg tcatcccgtc
     9421 gttcgcggtg ggacgggcgc aggacatttt gtacctcctc accgacctga cgcggaaagg
     9481 gcgcctatcg gggatcacgt tgtacatcga ctccccgctg gccgcggagg ccacgcggat
     9541 caccatgcgc caccccgagt gctacgatga cgagacgcgg gagctctttg cctggcggga
     9601 cgcccacccc gacgcactgg aggtcgttct ggtcagaaac atggaggagt cccgcgagtt
     9661 gaactccctg agggggggag ggatcctgat ggcggggagc gggatgtgcg acgcgggaag
     9721 gatcaagcac cacctgaagc acaacctgtg gcggaaggag tgcagcgtca tcatcgtcgg
     9781 gttccaggcc caggggaccc tcggccggaa gatcgtcgac ggcgcgaaac gggtgcgggt
     9841 cttcggggag gagatcgcgg tggccgcgga cgtgtacacg atcggtggtc tgtcggcgca
     9901 cgccgaccgg gacgacctcc ttgcgtgggc ggggaagttc caggcccctc cagggaacgt
     9961 cttcgtggcc cacggcgagg agtccgtctc cctcgaattc gccgggacgc tgaaagagaa
    10021 gctcgggtgg ctcgcgcagg tcccctcgcc cggccagccg ctgatcgtct gacccgaatg
    10081 atgatgcgtt ccatcctccg ccacgtactg ctctgcgttc tcgttgcgct tttccccggt
    10141 gccgcccatg cggcggaccc gcaggatgat atcctgcgcc agatcggcgt ggacgagaag
    10201 ccaggcgccc aggttcccct ggatctgccg ttcaccgatg cctcgggaag gacggtccgg
    10261 ctgggggact acctgcgcga cggcccggtc cttctcacgc tcaactacta tacctgcccc
    10321 atgctgtgtc ctttgacgtt ccgcggcctc gcggcaacga tgggggaggt aaaggacctg
    10381 tcgccgtcga aggattaccg catcgtcacc gtgagcatcg atcccgcaga gatcccggag
    10441 atcgtccggg cgaaggcgaa cgagtcgcac gccctggtgc ccgggcttaa ggatgcggac
    10501 tcccgctggc ctttcctgta cggagacgga gagacgatcc ggcgggtgac ggaggccgtc
    10561 ggctatcgtt acaagaaggt gggggcggag ttcgcccacc cctccgttca gatcgtcctc
    10621 tcccccggcg gcaaggtgtc ccgctacctg tacgggtttg agatcgcccc gcgggacttc
    10681 aagcttgccc tgctcgaggc gtcggaagga aagatcggcg catctaccgc gggaaacgcg
    10741 attctaatgt actgtttcag gtacgacccg gtcgggaaga agtacatgct gtacgcccgc
    10801 aacatcatga aggcggcggg tgcggtcacc ctggtcctgc tcgctgggct tctggtcgtc
    10861 ctgtggagga agtacgggag aacgccggga cccccgggga aaggggagtg atggacaacg
    10921 tccttgcgtt cggtgcagcc tccgcccagg cgggccgggt cgacgccgtt ttcctgatga
    10981 tcctcgtcat cggcgtattc ttcttttttc ttacccaagg gatactgatc tatttcgccg
    11041 tgaagtaccg ccgccgcctc ccgggccggg acaacgaaac gcccgccatc acgggcaacc
    11101 ccctgctcga gttcctctgg atcctgatcc cttccatcgt cgtcgtggtc atcttctact
    11161 atggctggcg cgtctacacc gaccagagga tccccgtcgc cggggcgacg gaggtatacg
    11221 tcaacgcgag gcagtggctg tacgagatca agtaccccga cgggcggacg gcgatcaacg
    11281 agatccgcgt accggcgggc aagtcggtga agttcatcct ctccgcggcc gacgttctcc
    11341 acggattcta cctgccggat ttccgggtga agatggacat ggtccccggc agggtgacca
    11401 ccctttgggt gcaaccggat cggcccggca gttatcaaat cttctgcacg gtgtactgcg
    11461 ggaccgggca ctcgaacatg ctggcccgat tgatcgtgat gcccccgcgg gaatacgcgg
    11521 aatgggtgga gcacggggga cgcgagggcg gcgagggcgg gaggcacgaa cctcttgccg
    11581 ttcgaggcga gcggatcttg aagaacgccg gttgttccaa ctgccacgcc gtcgacggga
    11641 agcagaagat ggcccccggc ttccgcgggc tgtacggctc gagggtccct ctcgagggag
    11701 gaataagcgt tacggccgac gaggaatatc tgcgggagtc gatcgtggat ccgggcgcaa
    11761 agatcgttaa ggggttcccc aacgtgatgc caacgttcaa gaccacgcta tcgaaagagg
    11821 acatcgacgc ggtggtggcg tacctgaaga cgcttaagtg aggcgatgag aggggtggta
    11881 ccgatggcgg aggagtccgg gaacggcagg
//
LOCUS       DASVJL010000383         7605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_158608, whole genome shotgun sequence.
ACCESSION   DASVJL010000383 DASVJL010000000
VERSION     DASVJL010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158608"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /gene="der"
                     /locus_tag="VF357_08080"
     CDS             complement(<1..822)
                     /gene="der"
                     /locus_tag="VF357_08080"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX7524145.1"
                     /translation="MSRAEFTVALVGRPNVGKSTLFNRMARKRRAITFDQPGITRDLV
                     AEPVEYDGRPFLLVDTGGYMTGEGDADLLPKIRGQVLRAVYESDLVVFLVDSRDGLLP
                     LDKEIAGMLRERGKPFLLAANKVDAKEGREGVSQFHALSVDTIHAVSAEHGTGVSELL
                     EAIVERIPARTGADEATDAKDADLPRIAVVGRPNVGKSTLVNTLAGFERVIASEIPGT
                     TRDAIDVMVERDGRKYLLIDTAGIRAKRKTEGAVEIFSVMKSLDSIKRCDLAILLI"
     gene            complement(809..1705)
                     /gene="era"
                     /locus_tag="VF357_08085"
     CDS             complement(809..1705)
                     /gene="era"
                     /locus_tag="VF357_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX7524146.1"
                     /translation="MNLKSGFVALLGRPNVGKSTLLNRIVRAKIAIVTRKPQTTRDRI
                     AGILTETRGQIVFLDSPGIHKPTRALNSHMVRTACRIGEEADIVAHVIDDRPVGKGPE
                     DAMVRGILEKIPVPRILVVNKVDRIGRAAADEVRKSLTRDDFYAASFLVSATKGDGVE
                     AFVTALFARLPEGPAFYPEDDLTDLPMRFIAKEVIREKLFEGLDEEIPYSIAVRIDEY
                     KEEPEKNLIRIRAEILVERDSQKGIVIGKGGAFLKKVGMASRLELEKETGSRVYLELF
                     VVVERDWSRSESMLRHLGYEPG"
     gene            complement(1702..2397)
                     /gene="rnc"
                     /locus_tag="VF357_08090"
     CDS             complement(1702..2397)
                     /gene="rnc"
                     /locus_tag="VF357_08090"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX7524147.1"
                     /translation="MTFSTFEERIGYRFSAPELLEEALRHGSAPGHEEGKRSYERLEF
                     LGDAVLNLCVAQEMYRMLPLAGEGVLTKARASIINNRNLAKVAERIGIPTSLRIDPSV
                     RRKGVGVTRKMAADAVEAVIGAIFLDGGHETALRFVRCHFRLPDLMGALVAGFDAKSR
                     LQEWCQGEHLPLPAYTLLSAVGPPHDRLFRVEVRVEGQPPAGGSGTSRKEAEMDAASK
                     AISFLLAAGGETP"
     gene            complement(2394..3674)
                     /locus_tag="VF357_08095"
     CDS             complement(2394..3674)
                     /locus_tag="VF357_08095"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEX7524148.1"
                     /translation="MRGRLSVVTVGCKANFADSAAILAHAARAGFEVVGGGAPADVLV
                     INSCTVTRRADRDCRALARRLRREHPGAVLVMTGCFAETTPEGRASVPEVDHWLGIRE
                     PSGLPQLLRSLAGDAPPPDEELSDFAADRLLGHSRVFLKVQDGCDAACAYCVVPKARG
                     AGRSLPRREVIERAVRAERDGAREIVLTGIHVGRYGVDRGERDGLAGLVEALLRATSG
                     CRFRLGSVEPLEITSALVSILSTQGRLCPHLHVPLQGGSDRVLLRMRRPYTARQYRER
                     LVLLTAEVPGIRLGADVIAGFPGETPDDFEETMRLIRDTPLSYLHAFPYSPRPGTESA
                     GWTDDVIAAEKTRRVARLRAADVAMRREYLGRQIGKTLVVAASARDPKTGEMRGTTEN
                     YAEAVFRAAIGARGNLIAVRIDAVRGELLEGTAV"
     gene            complement(3650..4738)
                     /gene="mnmA"
                     /locus_tag="VF357_08100"
     CDS             complement(3650..4738)
                     /gene="mnmA"
                     /locus_tag="VF357_08100"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX7524149.1"
                     /translation="MTKKRILAAMSGGVDSSVAALLLQRDGWDVIGISMDLYDYSAVT
                     RDREGTCCSLDDLYDARRVCDTLGIPYYVLNLRDEFRREVIDPFVREYQAGRTPNPCI
                     LCNEHLKFRALLRKADELGAEGVATGHYAVIRTETDGRCRLFASPNAGKDQSYFLYSL
                     DSDRLRRIRFPVGEMTKEQVRKIALGAGLPVYEKRESQDICFVTDDSYTRFLESRGIS
                     EDRGRFVDREGNFLGNHKGILRYTVGQRKGLGIASKEPLYVVAIDAGKNEIVLGSDGE
                     TFSGGATVISPTFVAGFPPGEEFRATARVRYHHPGAPCAVRTTGDRLAVTFDAPQRSV
                     TPGQALVFYEGDEVLGGGWIECAADSRS"
     gene            complement(4735..5913)
                     /locus_tag="VF357_08105"
     CDS             complement(4735..5913)
                     /locus_tag="VF357_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX7524150.1"
                     /translation="MRKIYFDHNASTPVHPEVAAAVQPFLGDLFGNPSSIHQAGRDVR
                     KAVEDARSEIAAFYGCRPLEVVFTSSGTESDNLAVKGVAYRPGNSGKHIVTSQVEHPA
                     IMNTCRFLETQGFRVTYVPVNRQGIVEPDAVRSALTKDTILVSVMAANNETGCLMPIA
                     EIGAIARNAGVLMHTDAVQATGKIPLPWETLPVDLLTFSGHKVNSLKGVGGLLARKGT
                     EIEAVLHGGHQERGRRGGTENVVGIVGMGKAFSLLSKNMAAEVAEVRRLRDAFERALF
                     ARVPDIVLNGHPTLRIPNTVNLSFRFVEGEALLLNLDMMGIACSSGSACTSGSLEGSP
                     ILLAMGADPTDSQGALRFSLGLGNTDDDVAYAVDAIETIVDKLRAMSPLYHPQRAKAR
                     "
     gene            complement(5910..6341)
                     /locus_tag="VF357_08110"
     CDS             complement(5910..6341)
                     /locus_tag="VF357_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7524151.1"
                     /translation="MKITTRGRYAVMAMVSLAASSRGNPVTIQTIAKGESIPEPYLQQ
                     LFLRLRKQNIVKSVRGPGGGFILARDPSEITVGEIIRTAEGKPARVGCRRSGRACGMI
                     DRCRTQGMWDALEARIEEFLDSMSVQDLFEEPRETREEVGA"
     gene            complement(6338..7018)
                     /gene="cysE"
                     /locus_tag="VF357_08115"
     CDS             complement(6338..7018)
                     /gene="cysE"
                     /locus_tag="VF357_08115"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX7524152.1"
                     /translation="MFRSIRNDIKVIFERDPAARSVLEIFLCYPGFHAVRFHHLAHWL
                     WTRDLRVLARFLSHISRTLTGIEIHPGATIGDGFFIDHGMGVVIGETSEIGNNVTLYH
                     GVTLGGTSWNKGKRHPTLEDNVIVGAGAAILGPIRIGHDSKIGSGSVVNKEVPPNSTV
                     VGIPGRVVYREGNVYNDPTGVGGTPDPEGKAIKCLTEQILALEKRVEELAKRIEETPH
                     PVEARTGT"
     gene            complement(7058..7513)
                     /locus_tag="VF357_08120"
     CDS             complement(7058..7513)
                     /locus_tag="VF357_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7524153.1"
                     /translation="MPIQGSRESSGGKRRAVLLLSLILLVWNAFATAQRLASVGFLPS
                     IDRTDGSPVHTSPTDNETAALSVESPRSGPLTIRQKYLLGKRVDINNASLLEISELPG
                     ISDKVAAAVVEERNRLGRFRSSVDLLGVKGIKEKRLQKILPFLSKMPNN"
BASE COUNT         1240 a         2666 c         2344 g         1355 t
ORIGIN      
        1 gatcagcagg atcgcgaggt cgcaacgctt gatcgaatcg aggctcttca tcacggagaa
       61 aatttcgacc gcgccttcgg tcttccgctt cgcgcggatc cccgcggtgt cgatcagcag
      121 gtacttcctt ccgtcccgct ccaccatcac gtcgatcgcg tcgcgcgtgg tgccggggat
      181 ctccgaggcg atgacccgct cgaaccccgc cagcgtgttg acgagcgtcg actttcccac
      241 gttcggccgc cccaccacgg cgatccgcgg aaggtcggcg tccttcgcgt ccgtcgcttc
      301 atcggcaccg gtccgggcgg gaatccgttc gacgatcgcc tcgagcagct cggatacccc
      361 cgtgccgtgc tcggcggaaa cggcatggat cgtatcgacg gagagggcgt ggaactggga
      421 taccccttcc ctgccttcct tcgcgtccac cttgttcgcg gcgagcagga acggctttcc
      481 ccgctcgcgc agcatcccgg cgatctcctt gtcgaggggt agcagcccgt cgcgggagtc
      541 gaccagaaag acgaccaggt ccgactcgta gaccgcccgc aggacctgcc cgcggatctt
      601 cggaaggagg tcggcgtccc cctcgcccgt catgtacccc cccgtgtcta cgagaaggaa
      661 agggcggccg tcgtactcca ccggctcggc gacgaggtcc cgcgtgattc ccggctggtc
      721 gaacgtgatg gcgcggcgtt tcctcgccat ccggttgaag agggtggatt ttcctacatt
      781 cgggcgcccc accagcgcca ccgtgaactc agcccggctc atatcccaga tgcctcaaca
      841 tcgattcgct cctcgaccag tcccgttcca cgacgacgaa cagttcaagg tatacccggg
      901 accccgtctc cttctccagt tcgaggcgcg aggccatccc gaccttcttc aggaacgcgc
      961 cgcccttccc gatcacgatc cccttctggg aatcccgttc cacgagaatc tcggcccgga
     1021 tgcggatcag gttcttctcc ggctcttcct tgtattcgtc gatccgcacc gcgatgctgt
     1081 aggggatctc ctcgtcgagc ccctcgaaga gcttttcacg gatcacctcc ttggcgataa
     1141 agcgcatcgg gaggtcggta aggtcgtctt cggggtagaa cgccggcccc tcgggcaatc
     1201 gcgcgaagag cgcggtgaca aacgcctcca cgccgtcccc tttcgtcgcg gagacgagga
     1261 aggacgcggc gtagaaatcg tcccgcgtga gactcttccg gacctcgtcc gccgccgcgc
     1321 gaccgatccg gtcgaccttg ttcaccacga gaatgcgcgg taccggaatc ttttcgagga
     1381 tcccgcgaac catggcatcc tccgggcctt ttcccaccgg acggtcgtcg atcacgtggg
     1441 cgacgatgtc ggcctcttcg ccgatccggc aggcggtgcg gaccatgtgg gaattgagag
     1501 cccgcgtcgg cttgtggatg cccgggctat cgaggaagac gatctgccct cgcgtctcgg
     1561 tgagaatccc cgcgatccgg tcccgcgtcg tctgcggctt ccgcgtgacg attgcgatct
     1621 tggcgcgcac aatccggttg agcaaggtcg attttccgac gttggggcgg cccagcaggg
     1681 cgacgaaccc ggatttcagg ttcacggggt ctccccgccg gcggccagga gaaacgatat
     1741 cgctttcgac gccgcgtcca tttccgcctc cttgcgtgac gttccgctcc cccctgccgg
     1801 cggctgcccc tccacccgga cctccacccg gaagagccgg tcgtgcggcg gcccgacggc
     1861 ggaaaggagc gtgtacgccg gaaggggcag atgctccccc tggcaccact cctgtaaccg
     1921 cgacttcgcg tcgaacccgg cgaccagcgc gcccatcagg tccggaagcc ggaagtggca
     1981 tcgcacgaac cgcagggccg tttcgtgtcc gccgtcgagg aaaatcgccc cgatgaccgc
     2041 ctccaccgcg tcggccgcca tcttccgggt gaccccgacc cccttccgac ggacggatgg
     2101 gtcgatccgc agcgacgtcg ggatgccgat ccgttccgcg accttcgcga ggttccggtt
     2161 gttgatgatc gaggcgcgtg ccttggtgag cacaccctcc cccgccagcg gcagcatccg
     2221 gtacatctcc tgtgcgacgc agaggttcag cacggcgtcc cccaggaact cgagccgctc
     2281 gtacgatcgc ttcccttcct cgtgcccggg agcggatccg tgccgcagcg cctcctccag
     2341 cagctccgga gcggaaaatc ggtatccgat ccgttcctcg aacgtggaaa acgtcaaacg
     2401 gccgtcccct ccaggagttc gccgcgaacg gcgtcgatgc ggacagcgat caagttcccc
     2461 cgcgcgccga tcgccgcgcg gaagaccgcc tcggcgtaat tttccgtggt cccgcgcatc
     2521 tccccggtct tcggatctct cgccgacgca gcgacgacca gcgtcttacc tatctgcctc
     2581 ccgaggtact ccctgcgcat cgcgacgtcc gccgcccgaa gacgcgcgac cctccgcgtc
     2641 ttctccgccg cgatcacgtc gtccgtccat cccgcgctct cggtcccggg tcggggggag
     2701 tacggaaagg cgtgaaggta gctcaacggg gtgtcgcgga tcagccgcat cgtctcctcg
     2761 aaatcgtccg gggtctcccc cggaaacccg gcgatcacgt ctgcccccag tcggatcccc
     2821 gggacctcgg cggtgagtaa aaccagtctc tcacgatact gcctcgccgt atacggccgg
     2881 cgcatccgga ggagaacccg gtcggagccg ccctgcaacg gaacgtgcag gtgagggcaa
     2941 agacgtcctt gcgtggaaag tatggaaacg agcgcagacg tgatctccag gggctcgacc
     3001 gaccccaggc ggaacctgca cccggaggtt gcccggagaa gcgcctcgac gagccccgcg
     3061 agcccgtccc gttcaccgcg gtcaactccg tagcgtccca cgtggatccc ggtcaagacg
     3121 atctcccggg cgccgtcccg ttccgcccgg accgcgcgct cgatgacctc ccgccgcggc
     3181 agggaccggc cggccccccg cgctttcggc acgacgcagt aggcgcacgc cgcgtcgcat
     3241 ccgtcctgga ccttcaaaaa cacgcggctg tgccccagca atcggtccgc cgcgaaatcg
     3301 gataattcct cgtcgggcgg aggcgcgtcc ccggccaggg atcggagcaa ctgcggcagg
     3361 ccggacggtt cgcggattcc gagccaatgg tccacctcag gcacggatgc gcggccttcg
     3421 ggggtcgttt cggcgaagca gccggtcata acgaggaccg cgcccggatg ctcgcggcgc
     3481 agccggcggg ccagcgcccg gcaatcccgg tcggcccggc gggtcacggt gcagctgttg
     3541 atgaccagga cgtcggccgg ggctccgccc cccaccacct cgaacccggc gcgtgccgcg
     3601 tgggcgagga tcgccgcgga gtcggcgaag tttgccttgc agccgacagt tacgaccgag
     3661 agtcggccgc gcattcgatc catcccccgc ccagaacttc gtccccttcg taaaagacga
     3721 gggcctgccc gggggtcacg ctgcgctggg gtgcgtcgaa ggtgaccgcg agacggtccc
     3781 ccgtcgtcct caccgcgcac ggcgcaccgg ggtggtggta tcgcacgcgg gccgtcgcgc
     3841 ggaactcctc ccccggggga aagccggcaa cgaaggtcgg ggagatgacc gtcgcccccc
     3901 cggaaaacgt ttccccgtcg ctgccgagga cgatctcgtt cttcccggcg tcgatcgcca
     3961 cgacgtagag cggctccttc gaggcgatcc cgagtccctt gcgctgcccg accgtgtatc
     4021 gcaggatccc cttgtggttc cccaggaagt tcccttcccg gtccacgaag cgcccgcggt
     4081 cttcgctgat cccccggctc tcgaggaacc gggtatagga atcgtcggtg acgaagcaga
     4141 tgtcctggct ctcccgcttc tcgtacaccg gcagcccggc accgagggcg atcttccgca
     4201 cctgctcctt cgtcatctcc cccacgggga accggatccg ccgcagccgg tcggagtcga
     4261 gcgaatagag gaagtacgac tggtcttttc cggcgttcgg cgaggcgaaa aggcggcaac
     4321 gcccgtcggt ttccgtacgg atgaccgcgt aatgccccgt ggcgactcct tccgccccca
     4381 gttcgtcggc ctttcgcaga agcgcgcgga atttcaggtg ctcgttgcac aagatgcacg
     4441 ggttcggcgt gcggcccgcc tgatactccc ggacgaacgg gtcgatcact tccctgcgga
     4501 attcgtcccg caggttgagg acgtagtacg ggatccccag ggtgtcgcac acgcgccgcg
     4561 cgtcgtagag atcgtccagc gagcagcatg tcccctcccg atcccgcgtc acggccgaat
     4621 agtcgtagag gtccatcgag atcccgatga catcccaccc gtcccgctgc agaagcagcg
     4681 ccgccacgga ggagtcgact cccccgctca tcgccgcaag aatccgtttc ttggtcatcg
     4741 tgctttcgct ctttgcggat ggtagagcgg cgacatcgcc cgcagcttgt cgacgatcgt
     4801 ttcgatggcg tccaccgcgt acgccacgtc gtcgtccgtg ttcccgagcc cgagggagaa
     4861 ccggagtgcg ccctgggaat ccgtcgggtc ggctcccatc gccaacagga tcggcgaccc
     4921 ctcgagcgac cccgaggtgc aggcggagcc cgaggagcag gcgattccca tcatgtcgag
     4981 gttcagcagc agcgcctccc cttccacgaa ccggaaagag agattgaccg tgttcggaat
     5041 ccgcagggtg gggtgaccgt tcagcacgat gtcggggacc cgcgcgaaca gcgcacgctc
     5101 gaacgcgtcc cgaagccggc ggacctcggc cacttccgcc gccatgttct tcgacaggag
     5161 ggagaacgcc ttccccattc cgacgattcc caccacattc tctgtgccgc cccgccgacc
     5221 ccgctcttga tgccccccgt gcagcaccgc ctcgatctcc gtccccttcc ttgcgagcag
     5281 cccccccacc cctttgaggc tgttcacctt gtgcccggaa aacgtaagca ggtccacggg
     5341 aagcgtctcc caggggagcg gaatcttgcc ggtcgcctgg accgcgtcgg tgtgcatgag
     5401 cacgccggcg ttcctcgcga tcgcgccgat ctccgcgatg ggcatcaggc aaccggtctc
     5461 gttgttcgca gccatgacgg agacgaggat cgtatccttc gtgagcgcgc tccggaccgc
     5521 gtcgggctcc acgatcccct ggcggttcac cgggacgtac gttacgcgga acccctgtgt
     5581 ttcgaggaac cggcacgtgt tcatgatcgc cggatgctcc acctgcgacg tgacgatgtg
     5641 cttccccgag ttccccggcc ggtacgccac ccctttcacc gcaaggttgt cggactcggt
     5701 accggagctc gtgaagacga cctcgagcgg gcggcatccg tagaacgcgg cgatctccga
     5761 tctcgcgtct tccaccgcct tgcggacgtc ccgccccgcc tggtggatgc tcgacgggtt
     5821 cccgaagagg tcgccgagga acggttgcac ggcggccgcc acctccgggt ggaccggggt
     5881 cgaggcattg tggtcgaaat agatcttcct caagccccca cctcctcgcg cgtttcccgg
     5941 ggctcctcga acaggtcctg cacggacatc gaatcgagga actcctcgat ccgggcctcg
     6001 agggcgtccc acatcccctg cgtccggcac cggtcgatca ttccgcacgc gcgccccgac
     6061 cgccggcagc cgacccgcgc gggcttccct tccgcggtcc ggatgatttc gccgacggtg
     6121 atctccgagg ggtcccgggc aaggatgaac ccgccgccgg ggccgcgaac gctcttcacg
     6181 atattctgct tgcgcagccg caggaagagc tgctgcaggt acggctcggg gatcgattcc
     6241 cccttcgcga tcgtctggat cgtcacgggg ttcccccgtg acgacgcggc cagcgagacc
     6301 atcgccatta cggcataccg tccccgggtg gtgatcttca cgttccggtc ctcgcttcca
     6361 cgggatgcgg cgtctcctcg atgcgcttcg ccaattcctc aacccgtttt tcgagggcta
     6421 aaatctgctc ggtcaggcac ttgatcgcct tcccttccgg atcgggcgtt cccccgacgc
     6481 cggtcggatc gttgtagacg ttcccttcgc gatagacgac gcgccccggg atcccgacga
     6541 ccgtcgagtt gggcggcacc tccttgttca ccaccgatcc ggagccgatc ttggagtcgt
     6601 gcccgatacg gatcggaccg aggatcgccg cccccgcccc gacgatcacg ttgtcctcga
     6661 gggtgggatg ccgcttcccc ttgttccagc tcgtcccgcc cagggtcacg ccgtggtaca
     6721 gggtgacgtt gttgccgatc tccgacgttt ccccgatcac cacccccatg ccgtgatcga
     6781 tgaagaaccc gtcgccgatc gtcgctcccg gatggatctc gatccccgtg agagttcgcg
     6841 agatgtggga aaggaaacgg gcgaggaccc ggagatcacg cgtccagagc cagtgcgcga
     6901 ggtggtggaa ccggacggcg tgaaaaccgg ggtagcagag gaagatctcg agcacgctgc
     6961 gcgcggcggg atccctctcg aatatgacct tgatgtcgtt ccggatcgat cggaacattt
     7021 tccctccctg tagcaaatta ttataagaca gattaggtca attatttggc attttcgaga
     7081 gaaagggaag gattttctgc agtcgcttct ccttgatccc cttgacgccg agaagatcta
     7141 cggaggagcg gaatcgcccg aggcgatttc gctcctccac cacggcggcg gcgaccttgt
     7201 cggagatccc gggcagttcc gagatctcca aaagggaagc attgttgata tctacccgtt
     7261 ttccaaggag gtatttctgt cgaatcgtga gggggccgct tcggggactt tcgacggaaa
     7321 gggcggccgt ttcgttgtcg gtgggggacg tgtggacggg agaaccgtcg gtcctgtcga
     7381 tcgaagggag aaacccgacc gaggcgaggc gctgagccgt ggcgaacgca ttccagacca
     7441 gcaggatcag ggatagaaga aggacggcgc gtcgcttccc gccgctactc tccctgctcc
     7501 cctgaatcgg cacgccgtgt cctccacagt ttgtaccgga tgctgtccac cagggcctgc
     7561 caggacgcct cgatgatgtt gtgcgacacg cccacggtgc cccag
//
LOCUS       DASVJL010000384         4230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_720148, whole genome shotgun sequence.
ACCESSION   DASVJL010000384 DASVJL010000000
VERSION     DASVJL010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720148"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VF357_08125"
     CDS             <1..543
                     /locus_tag="VF357_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7524154.1"
                     /translation="PLPGGIDIGSTKAMYYFALAVVGISVEGLRRIRSSPFGATLKAI
                     RENSQRAAYLGVNVNLYQWTAFVIAGAFTGLAGGLYAVMEKAITPDIIHWTKSGEPVF
                     MTIIGGIYTFAGPAVGAAVFVVLNSYLVAWTENWALVLGIILLVMVLMLPGGVVGFIN
                     ERARGALSKRGWVRKWVFSK"
     gene            525..1280
                     /locus_tag="VF357_08130"
     CDS             525..1280
                     /locus_tag="VF357_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7524155.1"
                     /translation="MGFLQVSRVVKSFGGLRALQDVSFSVERGEIRAIIGPNGAGKST
                     LFNVMTGLFPPDSGEVVFDGERISGVPPHRIIRKGIGRSFQITNIFPRMTVFENVQVA
                     LFSHHGKSRDAFGFARRYPTVAAEALAILGKVGLAEKHEMSASILSHGDQKRLEISIS
                     LASRPRLLMLDEPTAGMSRFESRETVELLRTISREQGLTLVFTEHDMDIVFGISEKIV
                     VLQQGSVIADGTPAEIKANPQVRKAYLGEEIQE"
     gene            1295..2002
                     /locus_tag="VF357_08135"
     CDS             1295..2002
                     /locus_tag="VF357_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7524156.1"
                     /translation="MALLELDSINTFYGRSHILFDVSLAVEKGEVVSLVGRNGAGKST
                     TFRSIIGLTAPQSGEVRFKGEKVNGLRAFRICRKGIGFVPEDRRCFSDLTVRENLEVA
                     ARREKVTTKPWSVERVYTLFPRLQEREKNLGSQLSGGEQQMLTIARTLMTNPEVLLLD
                     EPSEGLAPLVVSLLAEMILQIRKEGVTVLLAEQNLHFCAKVSDRAFVIDKGSVKFEGT
                     MKELLSNDEIKEKYLAV"
     gene            1992..3275
                     /locus_tag="VF357_08140"
     CDS             1992..3275
                     /locus_tag="VF357_08140"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7524157.1"
                     /translation="MPSDPGSFRTFAGRAFDPAAIPGKPEALAGVRVLEVATRIFGPA
                     TADYLGLFGAEVIKVEMPPRGDLMRYVAPEGFFWKEMSPAFLSLNRNKFHVGLDLHPA
                     EGKELFLRLAERSDVVVENLRAGTMDKWGVGYLQLRERNPRIVYAANSGFGQWGPYSA
                     GRASYDATAQAVSGFSAVTGFPDQPPMKAGFWVGDYTAALMSANAILAALAARRKTGE
                     GQMIDLSQAEAMIRTLDWTWPYAGLTGKDRARNGNVDSSYPPSGIYRCRDGFVAVSAR
                     DEDEMIPLAHAVGAADPEGARIVTPERVGAFCASRRVDEVVRIAEDAGFSAAPVRGGR
                     EQYHDPHLRARGTVCSVDDPLYGRVDEYGPAPKLSESPGRVKWSAKPVGWHNERVFGD
                     LLGMTAGETEALIRKKVIGTWADIPGARPPKGSAP"
     gene            3272..>4230
                     /locus_tag="VF357_08145"
     CDS             3272..>4230
                     /locus_tag="VF357_08145"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7524158.1"
                     /translation="MSGFSGWAREATEPSAAAGKPEALEGIRVLEFCPGHFGGMVAAS
                     VLAEFGAEAVKVEPPGGDPLRLVAPEEILVAGTGLPFLSEARNRRFVTLDADDSAGRD
                     VFRRLALSADVIITTEPPERMEAMGCDYLSLREERPGIVYLSLSTYGAFGPDASRPVR
                     DSDILCQALSGGPSIVGEPEREDGAPLPHQAPTRLGNWHGAFVQGLWGAYGVLAALHF
                     RAETGKGQAVDLSGAESLMMFADYNITWMHTGGKARERVGNFDPAVFPYTYIRCRDGY
                     TFIAAYNDEAFDSLMHIIGRPELARDPRFSTPKNRVALENERAL"
BASE COUNT          685 a         1356 c         1494 g          695 t
ORIGIN      
        1 ccgctccccg gggggatcga catcggctcc acgaaggcga tgtactactt cgccctcgcc
       61 gtggtgggga tctccgtgga gggcctccgg cggatccggt cctcgccctt cggggccacc
      121 ctcaaggcga tccgcgagaa cagccagcgg gcggcgtacc tcggggtgaa cgtgaatctg
      181 taccagtgga cggccttcgt gatcgccggc gccttcacgg ggcttgccgg cgggctgtat
      241 gccgtcatgg aaaaagcgat cacaccggac atcatccact ggaccaagtc gggcgaaccg
      301 gtgttcatga cgatcatcgg cgggatctac acgttcgcgg gaccggcggt cggtgcggcg
      361 gtcttcgtcg tgctgaactc gtacctggtg gcgtggaccg aaaactgggc gctcgtgctg
      421 gggatcatcc tcctcgtcat ggtcctcatg ctcccgggcg gcgtcgtcgg gttcatcaac
      481 gagagggcgc ggggcgcctt gtcgaagcgg ggatgggtgc ggaaatgggt tttctccaag
      541 taagccgcgt cgtgaagtcg ttcgggggtc tgcgcgcgct ccaggacgtc tccttctcgg
      601 tggagcgggg cgagatccgc gcgattatcg gcccgaacgg ggccgggaag tccaccctgt
      661 tcaacgtgat gaccggcctg ttcccccccg actccgggga ggtggtcttc gacggcgaga
      721 ggatctccgg ggttccgccc caccggatca tccggaaagg gatcggacgc tccttccaga
      781 tcaccaacat ctttccgcgg atgacggtct ttgagaacgt ccaggtggcg ctcttctccc
      841 accacggaaa aagccgcgac gcgttcgggt tcgcgcgccg atacccgacc gttgctgccg
      901 aggcactggc gatcctcggg aaggtggggt tggcggagaa gcacgagatg tccgcctcga
      961 tcctttccca cggcgaccag aagcggctcg agatctccat ctccctcgcc tcgcggcccc
     1021 gtctcctgat gctcgacgag ccgacggcgg ggatgtcccg cttcgagagc cgggagacgg
     1081 tggaattgct gcggaccatc tcccgggagc aggggctaac gctcgtgttc accgagcacg
     1141 acatggacat cgtcttcggc atctcggaaa agatcgtggt gctgcagcaa gggtcggtga
     1201 tcgccgacgg gacgccggcg gaaatcaagg cgaaccccca ggtgcgcaag gcgtacctcg
     1261 gcgaagaaat ccaggagtag gaacggggat cccgatggcg ctgctcgaac tcgattccat
     1321 caacacgttc tacggacgca gccacatcct cttcgacgta tccctcgctg tggaaaaggg
     1381 ggaggtcgtc tccctcgtcg gtcgcaacgg ggcggggaaa agcaccacgt tccgctccat
     1441 catcgggctg acggcgccgc agtcgggaga ggtccgcttc aagggggaga aggtgaacgg
     1501 cctgcgcgcg ttccggatct gccggaaggg gatcggcttc gttccggagg accggcgttg
     1561 cttctcggac ctcaccgtgc gtgagaacct cgaggttgcg gcgcgccggg agaaggtgac
     1621 gacgaagccc tggagcgtgg agcgggtcta caccctcttc ccgcggctgc aggagcggga
     1681 gaagaacctc gggagccagc tatccggcgg ggagcagcag atgctgacga tcgcccgcac
     1741 gctgatgacg aacccggagg tgctcctcct cgacgaaccg tcggaagggc tggcgccgct
     1801 ggtcgtatcc ctgctggccg agatgatcct ccagatccgc aaggaggggg tgacggtcct
     1861 cctcgcggag cagaacctgc acttctgcgc caaggtctcg gaccgcgcct tcgtgatcga
     1921 caaggggtcg gtgaagttcg aggggacgat gaaggagctc ctgtccaacg acgagatcaa
     1981 ggaaaagtac cttgccgtct gacccgggga gtttccggac cttcgccggg cgggctttcg
     2041 accctgcggc gatccctgga aaacccgaag cactcgcagg ggtgcgcgtc ctcgaggttg
     2101 cgacgcggat cttcgggccg gccaccgcgg actacctcgg cctcttcggc gccgaggtga
     2161 tcaaggtcga gatgccgccc cgcggcgact tgatgcggta cgtggccccc gagggtttct
     2221 tctggaagga gatgtcgccg gctttcctct ccctgaaccg gaacaaattc cacgtggggc
     2281 tcgacctgca cccggccgag gggaaggagc tcttcctgcg gctcgcggaa cggtccgatg
     2341 tcgtcgttga gaatctccgc gccggaacga tggacaagtg gggcgtcggg taccttcagc
     2401 ttcgggagcg aaacccccgg atcgtatacg ctgccaactc ggggttcggg caatgggggc
     2461 cgtactcggc gggacgggcg tcgtacgacg ccaccgccca ggccgtttcc ggtttctccg
     2521 ccgtcacggg gtttccggac cagccgccga tgaaggcggg gttctgggtc ggggactaca
     2581 ccgcggcgtt gatgtccgcc aatgcgatcc ttgcggccct cgccgcccga cggaagaccg
     2641 gcgaagggca gatgatcgac ctttcgcagg ccgaggcaat gatccgtacg ctggactgga
     2701 cgtggccgta cgccgggttg acgggaaagg atcgcgcgcg gaacgggaac gtcgactcct
     2761 cgtatccccc ctcggggatc taccgctgcc gcgacgggtt cgtcgcggtc tccgcgcggg
     2821 acgaggacga gatgatcccc ctcgcgcacg ccgtcggggc ggccgatccg gagggggcgc
     2881 ggatcgtcac gccggaacgg gtgggggcgt tttgcgcctc ccggagagtc gacgaggtcg
     2941 tgcgcatcgc ggaggacgcc gggttctccg cggcgccggt gcgcggaggg agggagcagt
     3001 accacgaccc gcacctgcgg gcccgcggga cggtctgttc ggtggacgat cccctttacg
     3061 gccgcgtgga cgagtacgga cctgcgccga agctatcgga gagcccggga agggtcaagt
     3121 ggagcgcgaa acccgtgggg tggcacaacg agcgggtctt cggggatctg ctggggatga
     3181 ccgccgggga gacggaagcg ctcatccgga agaaagtgat cgggacgtgg gccgacatcc
     3241 ccggcgcccg gccgccgaag ggaagcgcgc catgagcggt ttctcggggt gggcgcggga
     3301 ggcgacggag ccttcggccg cggcggggaa gcccgaggcg ctggaaggga tccgggtcct
     3361 cgaattctgc cccggccact tcggggggat ggtggccgcc tcggtcctgg cggaattcgg
     3421 cgccgaggcg gtcaaggtgg aacctcccgg tggcgatccg ctccgcctcg tggcgcccga
     3481 ggaaatcctt gtggccggga cggggctccc gttcctctcc gaggcgcgca accgccgctt
     3541 cgtcaccctc gacgccgacg attccgccgg gcgggacgtc ttccggcgcc tcgccctctc
     3601 cgcggacgtg atcatcacga cggagccgcc ggagcgcatg gaggccatgg ggtgcgacta
     3661 cctttccctg cgcgaggaac gcccggggat cgtctatctc tccctctcca cctacggcgc
     3721 gttcggtccc gacgcctccc gtcccgttcg ggacagcgac atcctctgcc aggcgctctc
     3781 cgggggtccg tccatcgtcg gggagccgga gcgggaggac ggcgctccgc tgccgcacca
     3841 ggccccgacc cgcctcggga actggcacgg ggcattcgtc caggggctat ggggagccta
     3901 cggggttctc gcagcgcttc atttccgcgc ggagaccggg aagggccagg ccgtggacct
     3961 ttcgggcgcc gagtcgttga tgatgttcgc ggactacaac atcacctgga tgcacacggg
     4021 gggaaaggcg cgcgagcggg tcgggaactt cgaccccgcc gtcttcccgt acacctacat
     4081 ccggtgccgg gacggctaca ccttcatcgc cgcctacaac gacgaggcgt tcgattcgct
     4141 gatgcacatc atcggccgac cggaactggc ccgcgacccg cggttctcca cgccgaagaa
     4201 ccgcgtcgcc ctcgagaacg aacgggcgct
//
LOCUS       DASVJL010000385         2867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_814634, whole genome shotgun sequence.
ACCESSION   DASVJL010000385 DASVJL010000000
VERSION     DASVJL010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814634"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1203)
                     /locus_tag="VF357_08150"
     CDS             complement(43..1203)
                     /locus_tag="VF357_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7524159.1"
                     /translation="MFKGIDYYDLESGFSSEARMIRDSVRDFVEKEFLPIVRDHYRAG
                     GFPMRIIPMLGEMGLLGSNLKGYGLPGIDQISYGLVMQELERGDSGLRSFASVQGALV
                     MYPIHTFGSEAQKERWLPALGAGKAIGCFGLTEPDHGSDPGGMKTKAVKDGDHYVLSG
                     AKMWITNGSVADVAVVWAKLDGVVRGFLVEKGMKGFSAPEIHSKLSLRASVTAELIFD
                     DVRVPAENILPGVQGLKGPLMCLTQARYGIAWGVIGAAMACFDEALSYTKERKMFGRP
                     VASFQLTQAKFADMLTGITSAQLIALQLGHLKEAGKMRPQQVSLAKRNNVRMALTVAR
                     TARGMLGANGISDEYQTMRHMCNLESVDTYEGTYEIHTLVMGKDVTGIDAVS"
     gene            complement(1248..2003)
                     /locus_tag="VF357_08155"
     CDS             complement(1248..2003)
                     /locus_tag="VF357_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7524160.1"
                     /translation="MEHSPGARLIDKAVDVLFLCRDAGGPVGVTETARRLGMPKSSAA
                     RIVAALVRRGFLRQEEPSGKYALSNIYYSFTSALSERNLLIPRAVPVMEKVFRALRET
                     IHLNVADGLERVCVHVLESPQQLKAVMPVGQRSPLYCGGSSKAILAFSDEETVERVFA
                     RPFRKFTGDTITGKKAMREEIHRIRERGYAVSHGERVEGITSVSVPVFHPGGQLAASL
                     TVSVPTARHTESLMGKILRELSAASAGITRGMG"
     gene            complement(2452..2667)
                     /locus_tag="VF357_08160"
     CDS             complement(2452..2667)
                     /locus_tag="VF357_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524161.1"
                     /translation="MVKHPPIGTDTLVGDILRQYPALREKVAELFGPDCISCRSNRHE
                     TITYTAWHKGLDPEEVVRTLNDALKGK"
     gene            complement(2669..>2867)
                     /locus_tag="VF357_08165"
     CDS             complement(2669..>2867)
                     /locus_tag="VF357_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524162.1"
                     /translation="SVIRLGKTIAVGSTEIRNAAGDPVAFGSATFYILNVRSPGDSPG
                     IMVATTGRSKPKTKPVLKGES"
BASE COUNT          474 a          993 c          871 g          529 t
ORIGIN      
        1 gggccgctca tcgcaatcct ccgccgcgca cgatcttcct tctcacgaca cggcatcgat
       61 gccggtcacg tctttcccca tcacgagggt gtggatctcg tacgtcccct cgtaggtgtc
      121 gaccgactcg aggttgcaca tgtgccgcat cgtctggtac tcgtcgctga tcccgttggc
      181 cccgagcatc ccgcgcgcgg tccgggcgac ggtgagcgcc atccggacgt tgttccgctt
      241 ggccaggctc acctgctgcg gacgcatctt cccggcctcc ttcaggtgac ccagctggag
      301 ggcgatgagt tgcgccgacg tgatccccgt cagcatgtcc gcgaacttcg cctgcgtgag
      361 ctggaaggag gcgaccggcc ggccgaacat cttccgctcc ttcgtgtagg agagcgcttc
      421 gtcgaagcac gccatcgcgg ccccgatcac gccccacgcg atcccgtacc gcgcctgggt
      481 caggcacatg agggggcctt tcagcccctg cacaccgggg aggatgtttt ccgccggcac
      541 ccggacgtcg tcgaagatga gctccgccgt gacggaggcc cgcagggaga gcttcgagtg
      601 gatctcgggc gccgagaagc ccttcatccc cttctcgacc aggaacccgc gcaccacgcc
      661 gtcgagcttg gcccacacca ccgccacgtc ggcgacggag ccgttcgtga tccacatctt
      721 cgcgccggaa agaacatagt gatccccgtc cttcaccgcc ttcgtcttca tcccgcccgg
      781 gtccgagccg tggtcgggct cggtcaggcc gaaacagccg atcgccttcc ccgcccccag
      841 cgccgggagc caccgctcct tctgcgcctc gctcccgaac gtgtggatcg ggtacatcac
      901 cagcgccccc tgcacggagg cgaagctgcg cagccccgag tcgccccgct ccagctcctg
      961 catgacgagg ccatacgata tctggtcgat cccgggcagc ccgtatcctt tcaggttgga
     1021 gccgagaagc cccatctccc ccagcatcgg gatgatacgc atcgggaatc ctcccgcccg
     1081 gtaatgatcc cgcacgatcg ggagaaattc cttttccacg aaatcccgga ccgaatcccg
     1141 gatcatgcgc gcctcggacg aaaacccgct ttccagatcg taataatcga ttcccttgaa
     1201 catcgtcccc tccttgcgtc gttttccccc ggccaacgcc gggggcgtta tcccatcccc
     1261 cgggtgatgc cggccgaggc ggccgagagc tcccgaagga tctttcccat caaggattcc
     1321 gtgtgccgcg ccgtcggaac ggagaccgtg agactggccg cgagttggcc gccgggatgg
     1381 aacaccggca cgctgaccga ggtgatcccc tcgacccgtt ccccgtggct gacggcgtac
     1441 ccgcgctccc ggatccggtg gatctcctct cgcatcgcct tcttccccgt gatcgtgtcc
     1501 ccggtgaact tccggaaggg acgggcgaag acccgttcca ccgtctcttc gtcggaaaac
     1561 gcgaggatcg ccttggaaga ccccccgcaa tacagcgggg accgctgccc gacgggcatc
     1621 accgctttca gttgctgggg agattccagg acgtggacgc agaccctctc gagaccgtcc
     1681 gcgacgttca gatggatcgt ttcccggagg gcgcggaaga ccttctccat caccgggacc
     1741 gcccgtggga tcaggaggtt tcgttcggaa agggcggagg tgaacgagta gtagatgttg
     1801 gagagggcgt acttccccga cggctcctcc tgccgcagga acccccgccg gacgagggct
     1861 gcgacgatcc gggcggcgct cgacttcggc atgccgaggc gccgggcggt ttccgtcacg
     1921 ccgacgggac ccccggcgtc ccggcacagg aacaggacgt cgaccgcctt gtcgatcagc
     1981 ctcgccccgg gagagtgttc cactatgtgg caccatgttc cataatcacg ataattatat
     2041 actcccggtg gggacttccg tcaaggtgtt tcacgttgct tcgtgctcca tttcataaat
     2101 acggcaacgt aaacggaggc gcttttatct tcgcgggata cccccttcct cgctacgttc
     2161 cggcagccct cccggcccgg ctccgaaggc catggatggc catcggggtt ccccgcgcag
     2221 tgccccccgt tcttcctacg gtgggggtac cccagcggcg cgtagctcgt actggggcgg
     2281 cgtctggagc gatgtggaga cagggatgtc gggaacgagc ggaggcgcag gcgaggagac
     2341 catggacggt cgacgagccg tagcggagag gaagcccccc gcgagcacga agtcccctac
     2401 gtccgattgt tgccgtattt atgaattgaa gcactcagga ggtttgtacg gttacttccc
     2461 cttcagcgcg tcgttcagcg tccggaccac ctcctccgga tcgagcccct tatgccaggc
     2521 ggtgtaggtg atcgtctcgt gccggttcga ccggcacgag atgcagtccg ggccgaagag
     2581 ttcggcgacc ttctcgcgta gcgccggata ttggcgcagg atgtcgccca ccaacgtgtc
     2641 ggtcccgatc ggcggatgct tgaccatgtc aactttcccc tttgagaacc ggcttcgtct
     2701 tcggcttcga ccggcccgtc gtcgccacca ttatacccgg agaatccccc ggcgagcgga
     2761 cgttcaggat gtagaaggtg gcggagccga aggcgaccgg gtcccccgcg gcgttccgga
     2821 tctccgtgga cccgacggcg atcgtcttcc cgagccggat caccgat
//
LOCUS       DASVJL010000386         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_720330, whole genome shotgun sequence.
ACCESSION   DASVJL010000386 DASVJL010000000
VERSION     DASVJL010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720330"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VF357_08170"
     CDS             complement(<1..410)
                     /locus_tag="VF357_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX7524163.1"
                     /translation="MFRKILIANRGEIAVRVLRACKEMGIRTVAVFSEVDRKALHTRY
                     ADEAYCIGPAPARESYLVIDKIIDVAKRTGAEAIHPGYGFLAETPLFADRREKEKIKL
                     IGPSAYAMRTMGSKTLARKTVQAAGVPVVPGTVEP"
     gene            complement(420..>1575)
                     /locus_tag="VF357_08175"
     CDS             complement(420..>1575)
                     /locus_tag="VF357_08175"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX7524164.1"
                     /translation="ARIQEGVQSLAGYADIFLRNTLTSGVVPQISAIMGPCAGGAVYS
                     PAITDFILMVKNTSYMFVTGPDVIKTVTHEEVTKENLGGAMAHNERSGVAHFAAEDEK
                     ECLYLIRELLCFIPQNNMEDPPAVTPKDSPDRADESLNQVVPDIPTKPYDIRDVIRKV
                     TDDGDFFEIQEHYARNIVIGFARMNGRSVGVVANQPAILAGCLDINSSIKGARFVRFC
                     DAFNIPLLTFVDVPGFLPGTTQEYGGIIKHGAKLLYAFAEATVPKVTVITRKAYGGAY
                     DVMASKHIRADVNFAFPTAEIAVMGPDGAVNIIFRKELVKSDNPEATKAELVADYRGR
                     FASPYKAAELGYIDEVIMPSETRAKVIKAFEMLKNKRDTNPPRKHGNIPL"
BASE COUNT          234 a          489 c          543 g          309 t
ORIGIN      
        1 ggctccacgg tcccggggac gacggggacg ccggccgcct ggaccgtctt ccgggccagc
       61 gtcttggagc ccatcgtccg catggcgtag gcggaggggc cgatcagctt gatcttctcc
      121 ttctcgcgcc ggtccgcgaa cagcggggtt tccgcgagga agccgtatcc ggggtgaatg
      181 gcctcggcgc cggtcctttt cgccacgtcg atgatcttgt cgatcacgag gtacgactcg
      241 cgcgccggcg cggggccgat gcagtacgct tcgtcggcat agcgggtgtg gagggccttc
      301 cggtcgacct cggagaagac cgccaccgtg cggatcccca tctccttgca ggcgcggaga
      361 acccggacgg cgatctcccc gcggttcgcg atcaggatct tccggaacac gtgtgcctcc
      421 tacaggggga tgttgccgtg cttgcgcggc gggttcgtgt cgcgcttgtt cttcagcatc
      481 tcgaacgcct tgatgacttt cgccctggtt tcagagggca tgatgacctc gtcgatgtaa
      541 ccgagctcgg ccgccttgta cggcgaggcg aaacgccccc ggtaatcggc caccagctcg
      601 gccttggtcg cctccgggtt gtcggatttg acgagttcct tccggaagat gatattcacc
      661 gccccgtcgg ggcccatcac ggcgatctcg gcggtcggga aggcgaagtt gacgtccgcc
      721 cggatgtgct tggaggccat gacgtcatac gccccgccgt acgccttgcg ggtgatgacg
      781 gtcaccttgg gaacggtcgc ttccgcgaag gcgtaaagga gcttcgcccc gtgcttgatg
      841 atcccgccgt actcctgggt cgtgcccggg aggaagcccg gcacgtccac gaaggtcagc
      901 agcgggatgt tgaaggcgtc gcagaagcgg acgaagcgcg cccccttgat cgaggagttg
      961 atgtcgaggc agccggccag gatcgcgggc tggttcgcca cgacgccgac ggaccgcccg
     1021 ttcatccgcg cgaacccgat gacgatgttc ctcgcgtagt gctcctggat ctcgaagaaa
     1081 tccccgtcgt ccgtgacctt cctgatcacg tcccggatgt cgtacggctt cgtcgggatg
     1141 tcggggacca cctggttcag gctctcgtcc gcacggtccg gggagtcttt cggcgtcacg
     1201 gcgggggggt cttccatgtt gttctgcggg atgaagcaga gcagctcgcg gatgaggtag
     1261 aggcactcct tctcgtcctc ggccgcgaag tgggccacgc cgctgcgttc gttgtgggcc
     1321 atcgcaccgc cgaggttctc cttggtaacc tcctcgtggg tgaccgtctt gatgacgtcg
     1381 gggccggtga cgaacatgta agaggtgttc ttgaccatca ggatgaagtc ggtgatcgcg
     1441 ggggagtaga cggcgccgcc ggcgcacggt cccatgatcg cggagatctg cgggacgacc
     1501 cccgaggtga gggtgttccg caggaagatg tcggcgtatc cggcgaggct ctggacccct
     1561 tcctggatgc gcgcg
//
LOCUS       DASVJL010000387         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1377635, whole genome shotgun sequence.
ACCESSION   DASVJL010000387 DASVJL010000000
VERSION     DASVJL010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377635"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VF357_08180"
     CDS             complement(<1..317)
                     /locus_tag="VF357_08180"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7524165.1"
                     /translation="MKTFDEELVKDRVNRFPFVKMMGMKLLSCSGGTSVMRCRVRQAL
                     KNSGGTLHGGVMGVLVDMSVATALRSVLPLTSRMTTVEYKVNLLKPVTDGVITARGSV
                     IRMG"
     gene            complement(571..1455)
                     /locus_tag="VF357_08185"
     CDS             complement(571..1455)
                     /locus_tag="VF357_08185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX7524166.1"
                     /translation="MWMSMTGFGRGERHGEEISVTVEARAVNHRFLDLHTRCPGRFLS
                     WEPRIRGIVRDSVRRGKIDLHVNVREWGKLGAGLRVNDELLRSFLGEAKRIREEHGVG
                     GKVTLRDLLSVPDLFQTAPVGGDPAELLWPVAEGAVRDALEMLGRARAEEGDRMKRVL
                     QESVARLEVLASEIRSLTAENKEQAAARFRERIAALSAEAGVDPVRLFQEAAILLDRL
                     DITEECDRLASHLTAVDALFRSPGDTVGKQIDFLVQEIFRELNTAGNKSAHARVSALV
                     VTAKTELEKIREQIQNIE"
     gene            complement(1591..2637)
                     /gene="rfaE1"
                     /locus_tag="VF357_08190"
     CDS             complement(1591..2637)
                     /gene="rfaE1"
                     /locus_tag="VF357_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX7524167.1"
                     /translation="MSDAGILSRAAEVVGRFPSRRVLVVGDIMLDEYVFGTVRRISPE
                     APVPVVAVTRETKVPGGAANVAFNLRGLGARVEMAGLLGDDAEGRFIARMLKGKRVGI
                     EAVVVELDRHTTVKTRVIAHSQQVVRVDREHKNPASRKASDALLRKTLSALAGVDGVV
                     FSDYRKGALTGELVREVTAAANRKGIFVAVDPKRPDFSFYRGCTVITPNKGEAQAALG
                     GRELATDLDVWEAGKDLLRAVRSKAVLITRGEEGMTLVERGRGACFHVPAFARQVFDV
                     TGAGDTVIGTLAACLAAGATMREAAVLANVAASVVVGEVGTSPITAEKLLRAAELRNS
                     EQEVRRSRRRRGAQ"
     gene            complement(2630..2800)
                     /locus_tag="VF357_08195"
     CDS             complement(2630..2800)
                     /locus_tag="VF357_08195"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX7524168.1"
                     /translation="MAMDKELLEILACPKCKGELQLTVDESELRCDACRLSFRVDDGI
                     PILLIDEATPYE"
BASE COUNT          442 a         1062 c          876 g          481 t
ORIGIN      
        1 cccatccgga tcaccgagcc gcgcgccgtg atcacgccgt cggtgacggg cttcagcaga
       61 ttgaccttgt actccaccgt cgtcatccgg gaggtcagcg gcaggacgga ccgcagcgcc
      121 gtggccacgg acatgtcgac cagcacaccc atcacgccgc catgaagcgt cccgcccgag
      181 ttcttcaggg cctgccgcac gcggcaccgc atcacgctcg tgccgccgct gcacgaaagc
      241 agcttcatcc ccatcatctt cacgaacggg aaccggttta ccctgtcctt gaccagttcc
      301 tcgtcgaacg tcttcatttc ccctcccaag tgctccccat catgaatacg ctaacgatct
      361 tcggctgcgc cgcctcggag ggcggggctc cgttcgtggc tcgccgtgcg agaacctgca
      421 cggctgcgca ccggcctcac tgtgcccccc gttcttccta ctgcgggggt accccaggga
      481 gtgtagttag tccgggagtg gggcgccccc cctcctgcgg cgactccgcc ggaccctcct
      541 gttgggtgca ccctgcgtac atagctctga tcactcgatg ttctggatct gctcgcggat
      601 cttttcgagc tcggtcttgg cggtcacgac gagggccgac acccgcgcgt gggcggactt
      661 gttcccggcg gtgttgagct cgcggaagat ctcctggacg aggaaatcga tctgcttccc
      721 cacggtgtcc ccgggggacc ggaacagcgc gtcgacggcc gtcaggtggg acgcgaggcg
      781 gtcgcactcc tcggtgatgt cgagccggtc gagaaggatc gcggcctcct ggaagaggcg
      841 gaccggatcg acccccgcct cggccgacaa cgcggcgatc cgctcccgga accgggcggc
      901 cgcctgttcc ttgttttccg cggtaagcga ccggatctcc gacgcgagga cctcgaggcg
      961 ggcgaccgac tcctggagga cccgcttcat ccggtcccct tcctccgccc gtgcgcggcc
     1021 gagcatctcg agggcgtccc ggaccgcccc ctcggccacc ggccaaagga gctccgcggg
     1081 atcgcccccg acgggggccg tctggaacag gtccggcacc gacagaaggt cccgcagcgt
     1141 cacttttccg ccgaccccgt gctcctcccg gatccgcttc gcctcgccga ggaacgaccg
     1201 cagcagctcg tcgttgacgc gcagtccggc gccgagcttc ccccactccc gtacgttcac
     1261 gtgcaggtcg atcttcccgc gccgcactga atcgcggacg atcccacgga tcctcggctc
     1321 ccacgacagg aaccggcccg ggcaccgggt gtgaaggtcg aggaagcggt ggttcaccgc
     1381 ccgcgcctct accgtgacgg agatctcctc cccgtggcgt tcgccccggc cgaaccccgt
     1441 catgctcatc cacataaacg tatctccaga atgacgatat aacagtctag tacaaagact
     1501 ccgggactca cccttcagct gcgtcgcctc ggagggcggg gctccgttcg tggctcgccg
     1561 tgcgatgaac ctgcacggct gcgctttacc tcactgcgcc cccctcctgc ggcgactccg
     1621 ccggacctcc tgttcactgt tgcgcagttc ggccgcgcgc aggagctttt cggcggtgat
     1681 cggcgaggtc ccgacctctc cgacgacgac gctggcggcg acgttggcga gcaccgccgc
     1741 ttcgcgcatc gtggctccgg cggcgagaca ggcggccaag gtcccgatta ccgtgtcccc
     1801 cgcgccggtc acgtcgaaca cctgccgcgc gaacgcggga acgtggaagc aggcgccacg
     1861 gccacgctcg accagcgtca tcccctcctc gccgcgcgtg atgaggaccg ccttcgaccg
     1921 gacggcccgg aggagatcct tcccggcctc ccagacatcg aggtcggtag cgagttcccg
     1981 ccccccgagg gcggcctgtg cctccccctt gttcggggtg atgaccgtgc agccgcggta
     2041 gaaggagaaa tcgggccgct tcggatcgac ggcaacgaag atccccttcc ggttcgccgc
     2101 cgcggtcacc tcccggacca gctctccggt cagcgccccc ttccggtaat cggagaagac
     2161 gacgccgtcg acaccggcga gggcgctcag cgtcttccgc aacagcgcgt cggacgcttt
     2221 tcgggacgcc gggttcttat gctcgcggtc cacgcgaacg acctgctggc tgtgggcgat
     2281 caccctcgtc ttgacggtgg tgtgacgatc cagctccacg accacagctt cgatcccgac
     2341 ccgtttcccc ttgagcatcc tggcgatgaa ccggccctcg gcgtcgtcgc cgagcagtcc
     2401 ggccatctcg acccgcgctc ccagtccgcg aaggttgaac gccacgtttg ccgcgccgcc
     2461 gggaaccttc gtctcccgcg tgacggcgac caccggcacg ggtgcctccg gagagatccg
     2521 gcggacggtt ccgaaaacgt actcgtcgag catgatgtcc ccgacgacga ggacccggcg
     2581 agaggggaac cgccccacca cctccgcggc gcgggacagg atcccggcgt cactcatacg
     2641 gcgtcgcttc gtcgatcagg aggatgggaa tgccgtcgtc gacgcggaag gaaagccggc
     2701 acgcgtcgca ccgaagttca ctctcgtcga cggtgagttg aagttccccc ttgcacttcg
     2761 gacacgccag gatctcgaga agctccttgt ccatcgccat cgcctacccc cccgccttcc
     2821 cggtcgacgg aatgagctcg ccgaccagcg tccaatcctt g
//
LOCUS       DASVJL010000388         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_910615, whole genome shotgun sequence.
ACCESSION   DASVJL010000388 DASVJL010000000
VERSION     DASVJL010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910615"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VF357_08200"
     CDS             <1..371
                     /locus_tag="VF357_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7524169.1"
                     /translation="MIRQQRGKVIHISSIFGAVGMNNQLAYACSKGGINQMTKVMAIE
                     WAPHNVQVNAIGPTYFETPLVATLRDDPERFRFINERTPMGRWGQPEELEGTVVFLAS
                     KASDFITGQTIYVDGGWTIW"
     gene            368..946
                     /locus_tag="VF357_08205"
     CDS             368..946
                     /locus_tag="VF357_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7524170.1"
                     /translation="MTGKGARMEAMEAILSRRSIRRYMPDPLPEETIRRILEAGMAAP
                     SAGDERPWHFVVITGKEMLAAIPEFHSLSRFVPNAAAAILVCADLALSKYPPADYWVQ
                     DCSAATQNILLAAHATGLGAVWLGVHPEKIRIDGFRALCRLPDSVAPFSLVSLGFPAV
                     RKEREHRFDPSRVHRERWSMPGETDAGARETT"
     gene            975..>1934
                     /locus_tag="VF357_08210"
     CDS             975..>1934
                     /locus_tag="VF357_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7524171.1"
                     /translation="MTVHGDGDRAMFRTIADIPHLQAQRRGGKTAVVTDAGDALTYAA
                     LDDRCRRFAAFLRSRSIAPGERVALLLQNVPEFVVSYFGGIEAGCVAVPVNYRLSPPE
                     VAYILADCTASVVVTTSEQFENVAGQEGAKGVKIWLLVDGPREGSLPFGDALSTEPVS
                     APAPAAPDDVAVLLYTSGTTGFPKGAMISHRNTLFNVDSCRATLGYREDDVGLLTLPL
                     FHVTGLHSQLVALLACGATVVLQKEYDTGQVLERIARNRVTALFFVPAIYKLFTLRND
                     IGRFDLSSVRIAAYGGAPMAPETIEALNRVMPARLHNCYGLTEC"
BASE COUNT          315 a          620 c          670 g          329 t
ORIGIN      
        1 acatgatccg gcaacagcgg gggaaggtca tccacatctc ctcgatcttc ggcgccgtgg
       61 ggatgaacaa ccagctcgcc tacgcctgca gcaagggcgg catcaaccag atgacgaagg
      121 tgatggcgat cgagtgggcg ccgcacaacg tccaggtgaa cgccatcggg ccgacgtact
      181 tcgagacccc cctggtagcg acgctgcggg acgacccgga gcggttccgg ttcatcaacg
      241 agcgtacccc gatgggccgc tgggggcagc cggaggagct cgaggggacg gtggtcttcc
      301 tcgcctcgaa ggcgtcggac ttcatcacgg ggcagacgat ctacgtcgac ggcggatgga
      361 cgatatggtg accggcaaag gagcgcgcat ggaggcgatg gaggcgatcc tgtcccgcag
      421 gagcatccgc aggtacatgc cggatccttt gccggaggag acgatccggc gtatcctgga
      481 ggcggggatg gccgctccgt ccgcggggga cgaacgtccg tggcacttcg tggtgatcac
      541 cggcaaggaa atgctcgcgg cgatcccgga gtttcattcg ctttcccggt tcgttccgaa
      601 cgcggcggcg gcgatcctcg tgtgcgcgga cctcgccctt tcgaagtacc cgccggcgga
      661 ttactgggtc caggactgct ccgccgcgac gcagaacatc ctgctggccg cccatgcgac
      721 ggggttgggg gcggtctggc tgggcgtgca tcccgagaag atacggatcg acggtttccg
      781 cgcgctttgc cgcctccccg attcggtagc ccccttttcg ctggtgtctc tcggattccc
      841 ggcggtgcgg aaagagaggg agcaccggtt cgacccctcg agggtccacc gggaacggtg
      901 gagcatgccg ggagagaccg acgccggcgc gcgcgaaacg acgtgatcga aaggtccgca
      961 tggagaggaa cgcgatgacc gtacacggag atggcgatcg cgcgatgttc cgcacgatcg
     1021 ccgacattcc gcatcttcag gcgcagcgaa ggggcgggaa aacggccgtc gtcaccgacg
     1081 cgggggatgc gttgacgtac gccgcgctgg acgaccgttg ccgcaggttc gccgccttcc
     1141 tccggtcccg ttccatcgct ccgggggaac gtgtcgccct cctgctgcag aacgtgccgg
     1201 aattcgtcgt gtcctacttc ggggggatcg aagcggggtg cgtggcggtg ccggtgaatt
     1261 accggctttc cccccccgag gtcgcctaca tcctggcgga ttgcaccgca tcggtcgtcg
     1321 tgacgacctc ggagcagttc gaaaacgtcg ccggccagga aggtgccaag ggggtgaaga
     1381 tctggctcct cgtggatggc ccgcgggaag gttcccttcc gttcggcgac gccctctcca
     1441 cggagccggt ctccgcgccg gcgccggcgg ctcccgacga cgtggccgta ctcttgtaca
     1501 cgtcggggac cacggggttt cccaaggggg cgatgatctc ccaccggaac accttgttca
     1561 acgtcgactc ctgccgcgcc acgttggggt accgcgagga cgacgtaggc ctcctcacgc
     1621 tgcccctgtt tcacgtgacg gggctccact cccagctggt ggcccttctg gcgtgcggcg
     1681 cgaccgtggt gctgcagaag gagtacgaca cggggcaggt tctcgagcgg atcgcccgga
     1741 accgtgtcac ggcgctcttt ttcgtccccg cgatctacaa gctgttcacg cttcggaacg
     1801 acatcggccg cttcgacctc tcgtcggtga ggatcgcggc gtacggcggc gccccgatgg
     1861 cgccggagac gatcgaggcg ctgaaccggg tgatgcccgc ccgactccac aactgctacg
     1921 ggctgacgga gtgc
//
LOCUS       DASVJL010000389         3570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_64626, whole genome shotgun sequence.
ACCESSION   DASVJL010000389 DASVJL010000000
VERSION     DASVJL010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64626"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..502
                     /locus_tag="VF357_08215"
     CDS             59..502
                     /locus_tag="VF357_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7524172.1"
                     /translation="MKLNKGSLFALFAVLELAGNGDRQLSTTDIAEKYGISTHHLAKV
                     MRNLVHSGLVQAVRGVGGGYRFAGNVSRTTLLDVIQLFEKLESELDMPNQGNPAGEPI
                     VAELRRITEEIDDLTKAILDTITLETALKSTRLRKEAAAAAFGKS"
     gene            complement(507..1325)
                     /locus_tag="VF357_08220"
     CDS             complement(507..1325)
                     /locus_tag="VF357_08220"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7524173.1"
                     /translation="MRIDFHVHMGVYSFHNPWVTEWMKESYPEGYEEYIRKYNDPGAF
                     EELLAAEGVDYACVLAELSPATTGLCTNEQVRDFCRGRRRLIPFCDISPNLFTDLGGE
                     LRRKVEDDGFRGLKLYPSYQHYYPNEQRMYPLYQAAQELGIPVLFHIGSSVFRGTRMK
                     YCDPLHLDDVAVDFPDLNLVMAHAGRGFWYDRAFFLAKLHANVYLEISGLPPSKLMTY
                     FPELDRLTGKVIFGSDWPGMRWIRRNMEAIGKLPLPPEGVERILGGNAAKLLRI"
     gene            complement(1325..2044)
                     /gene="fabG"
                     /locus_tag="VF357_08225"
     CDS             complement(1325..2044)
                     /gene="fabG"
                     /locus_tag="VF357_08225"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX7524174.1"
                     /translation="MGDFLNLEGRIAVVTGGARGIGRAITRALIGHGVRVHVFDVAPV
                     KGNDMAPYSFHQVDIADSASVAKAVAALPAGVSLLVNNAGITRDRSAVNMSDDEWDSV
                     LSVNLTGAFHMIRALAPAMRMAGYGRIVNLTSINGIRGKFGQANYCASKAGLIGLTKT
                     LARELGPKGITVNAVAPGMVMTEMALALPPEIVDKAKTEAVLPELATPEDVANTVLFL
                     LSDAARMITGEVIRVDAGQYV"
     gene            2225..3202
                     /locus_tag="VF357_08230"
     CDS             2225..3202
                     /locus_tag="VF357_08230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7524175.1"
                     /translation="MADLQLRSLHRDDLDRVSGIESRITGRSREGFLEKRFAAAVGLP
                     VGFIAFAAVRDGKLAGYAIARIQEGEFGVPGAVAVLDVIGIDPDAQGKGIGKALLAEM
                     EQRMKAREIGTLRTQVDWGNPAMIRFFSSSGFLLAAAQILERDTSPLREEIREVGSAG
                     AEVRSPMRAAGDDYETLSRDRVPVRSLKEDDLAAVARIDRKLTGRDRSAYYAAKLREM
                     LTESGIRVSLVAEEDGLVVGFVMARVDFGEFGKVDKSAILDTIGVHPGFAGSGIGHAL
                     LSQLFLNLATLQVETVLTQVSPENIDLQRFLYSCGFHPSQRLMLSKTIS"
     gene            3296..>3570
                     /locus_tag="VF357_08235"
     CDS             3296..>3570
                     /locus_tag="VF357_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524176.1"
                     /translation="MTTTDGIINKSAPSQLVDHNLVAQDVESLCGGMVRYEKRPAKRR
                     DGSAAEGLVNAWIVINNPKQYNSYTTDMVKALILAFRRASVDRGVNAV"
BASE COUNT          661 a         1148 c         1136 g          625 t
ORIGIN      
        1 tcttccggag ccaggggctg cgctcgggcc gatacatcga actggaacgg gagatcgatt
       61 gaagctgaac aagggatcgc tgttcgccct cttcgcggtg ctcgaacttg cggggaacgg
      121 cgaccgccaa ctctcgacca ccgacatcgc cgagaaatac gggatctcca cgcaccatct
      181 cgccaaggtg atgcgcaacc tggtgcactc ggggctggtt caggcggtgc gcggcgtcgg
      241 cggcgggtac cggttcgcgg ggaacgtcag ccggacgacg ctgctggacg tgatccagtt
      301 gttcgagaaa ctcgaatccg agctcgacat gccgaaccag gggaacccgg caggggaacc
      361 gatcgtcgcg gagcttcgcc gcatcacgga agagatcgac gacctcacga aggcgatcct
      421 cgacacgatc acgctggaaa cggcgctgaa aagcacccgc ctccgcaagg aagccgccgc
      481 ggccgcgttc gggaaatcgt gacccgttag atccgcagga gcttcgcggc gttgccgccg
      541 aggatccgct ccaccccttc cggcggcaac ggcaacttcc cgatcgcttc catgttgcgc
      601 cggatccacc gcatccccgg ccagtcgctg ccgaagatca cctttcccgt gaggcggtcc
      661 agttccggaa aatacgtcat cagcttcgac gggggcaagc cggagatttc caggtagacg
      721 ttcgcgtgca gcttcgcgag gaagaacgcc cggtcgtacc agaatccccg gccggcgtgc
      781 gccatcacca ggttcagatc cgggaaatcg accgccacgt cgtcgaggtg cagcgggtcg
      841 caatacttca tccgggtccc gcggaacacc gacgacccga tgtggaagag gacggggatc
      901 cccagttcct gcgccgcctg gtacagcggg tacatcctct gttcgttcgg atagtagtgc
      961 tgatacgagg gataaagctt cagcccccgg aaaccgtcgt cttccacctt ccgccgcagc
     1021 tcgcccccga ggtcggtgaa caggttgggg ctgatgtcgc aaaaggggat cagccggcgc
     1081 cggccccggc agaaatcgcg cacctgctcg ttcgtgcaga gccccgtggt ggcaggactc
     1141 agctccgcca gcacgcaggc gtagtccacc ccctcggccg ccagcagttc ctcgaaggcc
     1201 ccggggtcgt tgtacttccg gatgtattcc tcgtatccct cggggtacga ctccttcatc
     1261 cactccgtca cccagggatt atggaaggag tacaccccca tgtggacatg gaaatcgatc
     1321 cgcatcagac gtattgcccg gcgtcgaccc ggatcacttc gccggtgatc atgcgggccg
     1381 cgtcggacag gaggaacagg accgtgttcg cgacgtcttc gggagtggcc agctccggca
     1441 gcaccgcctc cgtcttcgcc ttgtcgacga tttcgggcgg aagcgccagc gccatctccg
     1501 tcatgaccat gccgggggcg acggcgttga ccgtgatccc cttgggaccc agttcccgcg
     1561 ccagggtctt cgtcagcccg atcagtcccg ccttcgaggc gcagtagttc gcctgcccga
     1621 acttcccgcg gatcccgttg atcgaggtga ggttgacgat ccgcccgtac ccggccatcc
     1681 gcatcgccgg cgccagcgca cggatcatgt ggaacgcccc cgtcaggttg accgagagga
     1741 ccgagtccca ctcgtcgtcg ctcatgttga ccgcgctgcg gtcgcgggtg atcccggcgt
     1801 tgttgaccag cagggagacg ccggccggaa gcgcggccac cgccttcgcc acgctggcgg
     1861 agtcggcgat gtcgacctgg tggaacgaat acggcgccat gtcgttccct ttcacggggg
     1921 cgacatcgaa cacgtgcacg cggacgccgt gaccgatcag ggcgcgggtg atcgcccgcc
     1981 cgatcccgcg ggccccgccg gtcacgaccg cgatccgtcc ctccagattc aggaaatcgc
     2041 ccatggctcc ccccactctg ttccaggtaa ttacgtattc aagtacccca ttgccttctt
     2101 gcctgtcaag gaggcccgcc ggaagaatcg aagaatggga ttgcgccggc agcggcgaag
     2161 gtggcaagtt atcggaggat ccggcggacg caacgcacgg aacgatcgag agaggagaac
     2221 gcgaatggcc gacctgcaac tccgctccct tcatcgggac gacctcgacc gtgtttccgg
     2281 aatcgagagc cggatcaccg gtcgctcgag ggaaggcttc ctggagaaac ggttcgcggc
     2341 agcggtggga ttgccggtcg gtttcatcgc cttcgccgcg gtgcgggacg ggaaactcgc
     2401 cggatacgcg atcgccagga tccaggaagg agaattcggc gtcccgggcg cagtggcggt
     2461 cctcgatgtc atcggcatcg accccgacgc ccaggggaag gggatcggga aagcgctgct
     2521 ggccgaaatg gagcagagga tgaaggcgcg ggagatcggg acgctccgga cccaggtcga
     2581 ctggggaaat cccgcgatga tccgcttctt ctcctcctcg gggttcctcc tcgccgccgc
     2641 ccagattctc gagcgggaca cctccccgtt gcgtgaggag atccgggagg tcggaagcgc
     2701 gggagcggaa gtgcggtcgc cgatgcgtgc cgctggggac gactatgaga cattgtcccg
     2761 tgaccgcgtc ccggtgcgct cgttgaagga ggacgacctc gcggcggttg cccggatcga
     2821 ccggaagctc accggccggg atcgttccgc ctattacgcc gcgaagcttc gggagatgct
     2881 gacggaatcc gggatccggg tttcgctggt ggccgaggag gacgggctcg tcgtcggatt
     2941 cgtcatggcg cgggtcgact tcggagaatt cggcaaggtc gacaagtccg ctatcctgga
     3001 caccatcggc gttcaccccg gcttcgcggg ttccggcatc gggcacgcct tgctctcaca
     3061 actcttcctg aaccttgcca ccctccaggt tgaaaccgtg ctcacgcagg tatccccgga
     3121 aaatatcgac ctgcagcgct tcctgtactc ctgcggcttc cacccttccc aacggctgat
     3181 gctgagtaaa acgatttcgt aactaatggg aagtgggttg ggacaaagtc cttgacatgt
     3241 taattggtat tgtggtactt gtttacttaa ttattccgta cctcgaggag gaggcatgac
     3301 gacaaccgac ggtatcatca ataaaagcgc gccgtcccaa ctcgtcgatc acaacctggt
     3361 cgcccaggat gtggagagcc tttgcggcgg gatggtccgt tacgagaagc gtcccgcgaa
     3421 gcgccgcgac ggctcggcgg ccgaggggct cgtaaacgcc tggatcgtaa tcaacaaccc
     3481 gaagcagtac aactcctaca cgacggacat ggtcaaggcg ctgatcctcg ccttccgccg
     3541 ggcctccgtg gaccgcgggg tgaacgccgt
//
LOCUS       DASVJL010000390         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_64672, whole genome shotgun sequence.
ACCESSION   DASVJL010000390 DASVJL010000000
VERSION     DASVJL010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64672"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VF357_08240"
     CDS             complement(<1..402)
                     /locus_tag="VF357_08240"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX7524177.1"
                     /translation="MYRAEKVSSIILIAFGLFVAFYSRQYLKLGKMITPGAGFLPYWI
                     GIALAVLGAIWFLNTLLSRRGSGAGGVGSDAGDAAAEREALRSRILWKFLPGVLLIIL
                     YAWLFEKAGYIVSTALFMVGWQKVVEREGWLK"
     gene            complement(404..1306)
                     /gene="dapA"
                     /locus_tag="VF357_08245"
     CDS             complement(404..1306)
                     /gene="dapA"
                     /locus_tag="VF357_08245"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX7524178.1"
                     /translation="METGFVRGVIPPIITPVDGDERVDEGALRRMVDHVIAGGVHGIL
                     SLGSNGEFFGLDRDEQRRAVAVTMDHVGGRVPVYVGIGAISTKECVRTAAMAGSLGAQ
                     AVTILPPMFLSPNDEELYHHFLAVAESTDLPVLLYNNPDRMRVNLSVSLVERLADVPN
                     IVGVKDSSGDLVLTAEYIRRTRRKGFRVLAGRDVMILGTLVYGGVGCVAATANIVPAL
                     VVEIYEKFLAGDLEGAREAQFRLAPLRMAFNLGSFPVVTKEALNLLGFEVGEPIRPNT
                     RCTEAYRARLRDVLVQVGALKGES"
     gene            complement(1319..>1636)
                     /locus_tag="VF357_08250"
     CDS             complement(1319..>1636)
                     /locus_tag="VF357_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX7524179.1"
                     /translation="NHFDISLAMFTHVGAAAPGKIAALDTHWIWQEGQERLTKEPFKI
                     VGGMVSVPDKPGLGVEADMAQIERAHQLYLKEGQGARDDAMAMQYLIPGWKFDNKRPC
                     LVR"
BASE COUNT          312 a          556 c          466 g          302 t
ORIGIN      
        1 cttcaaccat ccctcccttt ccaccacttt ctgccacccg accatgaaaa gggcggtgga
       61 aacgatgtac ccggcctttt cgaacagcca ggcgtagagg atgatcagca gtacacccgg
      121 cagaaacttc cagaggatgc ggctgcgcag cgcttccctt tccgccgccg catctccagc
      181 gtcgcttccc actccgcccg cccccgatcc cctccgggaa aggagggtat tcaggaacca
      241 gatcgccccc aggacggcca gcgcgatgcc gatccaatac gggagaaatc ccgcgccggg
      301 agtgatcatc tttcctagtt tcagatactg gcgtgaatag aaggccacga agagtccgaa
      361 tgcgatcaag atgatcgagg agaccttctc cgcgcggtac atctcagctt tcccccttca
      421 gcgcgcccac ctggaccagc acgtcgcgca acctcgcccg gtaggcctcc gtgcagcggg
      481 tattcggccg gatcggctcc ccgacctcga aacccagcag gttcagggct tccttggtaa
      541 ccaccgggaa gctgcccagg ttgaacgcca tccgcagggg tgcgaggcgg aactgtgcct
      601 cccgggctcc ctccaggtcg ccggccagga atttctcgta gatctccacc acgagggccg
      661 gaacgatgtt cgccgtcgcg gcgacgcaac cgacgccgcc atatacgagg gtcccgagga
      721 tcatgacgtc cctgccggcc agtacccgga accctttcct ccgggtccgg cggatatact
      781 ccgcggtcag caccaggtcg ccgctgctgt ccttcactcc gacgatattc ggcacgtcgg
      841 ccagccgttc cacgagggac accgaaaggt tgacccgcat ccggtcgggg ttgttgtaga
      901 gaaggactgg cagatcggtc gactccgcga ccgcaaggaa gtggtggtac agttcctcgt
      961 cgttgggcga gaggaacatg ggggggagga tggtgacggc ctgtgcaccc aacgatcccg
     1021 ccatcgcggc cgtccggacg cactccttgg tggagatggc gccgatcccc acgtagacgg
     1081 ggacacgtcc gccgacatga tccatggtga cggccacggc gcgccgctgt tcgtcacgat
     1141 ccagtccgaa gaactcgccg ttgcttccga gcgagaggat cccgtggacc cctccggcga
     1201 tcacgtgatc gaccatccgc cgcaaggcac cttcatccac ccgttcgtcg ccgtccaccg
     1261 gggtgatgat gggggggatc accccacgaa caaaacccgt ctccatggtc gttccctttc
     1321 agcgcacaag gcagggacgc ttgttgtcga atttccagcc cggaatcaga tactgcatgg
     1381 ccatggcgtc gtcgcgcgcg ccctgtcctt ctttcagata tagttgatgc gctctctcga
     1441 tttgcgccat atccgcttcc actcccaggc cgggcttgtc gggaacggac accatcccgc
     1501 ctacgatctt gaacggctct ttcgtcagac gctcctgacc ctcctgccag atccaatgcg
     1561 tgtccaatgc ggcgattttc cccggcgcgg cggctccgac atgggtaaac atggccaacg
     1621 agatatcaaa atgatt
//
LOCUS       DASVJL010000391         5253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1003321, whole genome shotgun sequence.
ACCESSION   DASVJL010000391 DASVJL010000000
VERSION     DASVJL010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003321"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /gene="hemG"
                     /locus_tag="VF357_08255"
     CDS             complement(<1..1206)
                     /gene="hemG"
                     /locus_tag="VF357_08255"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX7524180.1"
                     /translation="MPRIVIIGAGLSGMATAHYLVKSLSDAGKEAEILLLEADAVPGG
                     KMRTIRQDGFAMEWGPNGFLTNKPHGMELVKDLGIGGRLARSSDLARKRFILSGGKLH
                     LLPETPPAFFTSQLLSLPGRLRILWEPFAPGPPAGVDESLGEFARRRLGPEALEKLID
                     PMVTGIFAGDPDKMSLRSCFPVIYDLERKYGGLVRGMLGVRKERAKQGVKGEMSAGPG
                     GVLMSFDHGVQALTDTLAGRLSEGLHLNVAVDRIERHGEAYVLSMSADGKREEMAANV
                     VVIAAPAYVAAGMVSPLDEGLSELLAGIPYSPITVAALGYRKATMGNPLDGFGFLIPR
                     GEKRKILGALWDSSVFPNRAPEGTALLRVMVGGVRAPALATLPEAELLAIARKELQEI
                     MGISAEPVLA"
     gene            complement(1206..2174)
                     /gene="hemH"
                     /locus_tag="VF357_08260"
     CDS             complement(1206..2174)
                     /gene="hemH"
                     /locus_tag="VF357_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX7524181.1"
                     /translation="MPAEGPFTGVVLLNMGGPDSLNAVRPFLARLFSDKELIRLPAAP
                     ITQPLFAWIVSGLRARKVRRYYEEIGGGSPIAAITERQRFALEEALRATGGRFKVYTG
                     MRYWFPLAKHAALEMKEDGVARAIALPLYPHFCSATTGSSFSDLRRWMKWAGCSCPLT
                     EIRSYPEHPKYVAALAETIAAVIREADRGGIFLLFSAHGVPQSLIDGGDPYREETERT
                     VAAVMRSFPGLPHGVSYQSKVGPTAWLPPDTVSEVTRLGREGVKTLVVVPVSFVSEHI
                     ETLHELDVRLAAHARQAGIQSFLRAPALNDAPGFIAALKDLVLGAV"
     gene            complement(2201..3235)
                     /gene="hemE"
                     /locus_tag="VF357_08265"
     CDS             complement(2201..3235)
                     /gene="hemE"
                     /locus_tag="VF357_08265"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX7524182.1"
                     /translation="MTEYRFLKACRREPVDVIPVWIMRQAGRYLPEYQKIRGNNSFLT
                     MCKTPELAAQVTIQPVERLGVDAAILFSDILIPVEAMGVPLEFHDGKGPMLGKAIRGQ
                     KDVDSLLVPDPAEKVPFVMEAIRILRKELDGKVPLIGFSGAPFTLASYIVEGGTSKNF
                     IQLKKLMYQAPEVYRSLMNKIAKTVILYLNAQIAAGAQAVQIFDTWAGVLTPGDYEEY
                     ALPYTRQVVSGLNRKGVPVIHFANDCATLLTAIKTLPIDVVAVDWRIPLDVAAMVVGP
                     DKALQGNMDPTMLFHPPEKIDDCVRDVLRRGEKASSHIFNLGHGILPPTNPEHAIAMV
                     EAVHRIGRKV"
     gene            complement(3253..4392)
                     /locus_tag="VF357_08270"
     CDS             complement(3253..4392)
                     /locus_tag="VF357_08270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7524183.1"
                     /translation="MRVYDLRKGYTLAKEEFIPKWIAWEVTGRCNLNCIHCRASSSMA
                     SHDSDFSLPEAKQLIDDIAEISKPVLVLSGGEPLLRKDLFEIAKYGTEKGFRMCIATN
                     GVLVTDEVVGKMKESGIRICSLSLDGSTAAVHDDFRKQPGAFEATLRAAEIFNRNGMQ
                     FIVNSSFTQRNQHDIAATCKLAKAIGAHAWYMFMIVPTGRGEAIMSELISKEDYEEIL
                     EWHYQMEKEETEMLVRPTCAPHYYRVRLQKMKEEGEKFQPRSLTFSTGGGKGCICAQS
                     ICFIDSKGNVQPCSYFPVVAGNVKKQKFGEIWRKSELFESLRDFEKYKGRCGECEYIN
                     VCGGCRARADAVLEDYLEEEPFCNYVPIKTRKRLAEAVQAGKSTK"
     gene            4423..>5253
                     /locus_tag="VF357_08275"
     CDS             4423..>5253
                     /locus_tag="VF357_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEX7524184.1"
                     /translation="MARKRRKRYHSPGTQPGTLAAHAEAGDAPVRVTSFQYDASRCEE
                     RTVAPSEIPSLSPPEGGVLWLDVCGLSDPSVVRAIGDRFRIHSLALEDVLNVPQRPKV
                     EWYGDHLLVVLSEIRYPDLPEQMAFLLADRVVVSFQERPGDAFEPVRERLRQGKGRVR
                     AEGADYLLYSLCDAVLDAFFPTLEHLGDEVEEMEERVLISPVPETFLAIRRLKRELMA
                     VRHAVWPARDALNLLLIEEHALIRPGTKVFFRDCYDHTIQLMDMVETFREMASGLVDE
                     Y"
BASE COUNT          854 a         1807 c         1699 g          893 t
ORIGIN      
        1 cgcgaggacc ggctcggcgg agatccccat gatctcctgc aactccttcc gggcgatggc
       61 gagcagttcc gcctcgggca gcgtcgcaag cgcaggcgca cgcacccccc ccaccatcac
      121 gcgcaacagg gccgttccct ccggcgcccg gttcgggaag acgctcgaat cccacagcgc
      181 ccccaggatc ttccgcttct ccccgcgcgg gataaggaag ccgaatccgt cgagggggtt
      241 gcccatcgtg gccttccgat accccagcgc cgccacggtg atcggcgaat aggggatccc
      301 ggcgaggagc tccgaaagtc cctcgtccag cggggagacc atcccggcgg ccacgtacgc
      361 cggcgcggcg atcaccacca cgttcgccgc catctcctcc cgcttgccgt cggcggacat
      421 cgaaaggacg tacgcttctc cgtgccgctc aatccggtcc acggccacgt tcaggtgcaa
      481 cccctccgag agccgcccgg cgagggtgtc ggtcagcgcc tgcaccccgt ggtcgaacga
      541 catcagcacg ccgcccggtc ccgcggacat ctctcccttc acgccctgtt tcgcccgctc
      601 cttccgcacg ccgagcattc cgcgcacgag cccgccgtac ttcctctcca ggtcgtagat
      661 cacggggaag caggagcgca gcgacatctt gtccgggtcg ccggcgaaga tgccggtgac
      721 catcgggtcg atcagcttct ccagcgcctc gggaccaagc cgccgcctcg cgaactcccc
      781 gagcgactcg tcgacgcccg cgggcggacc cggtgcgaac ggctcccaca ggatccgcag
      841 gcggccggga agagagagca gctgcgacgt gaagaacgcc ggcggcgtct cggggaggag
      901 gtgcagcttc ccgccggaga ggatgaaccg tttgcgggcg aggtcggagg agcgggccag
      961 gcgacccccg atgccgaggt ccttcaccag ctccatgccg tgcggcttgt tcgtgaggaa
     1021 gccgttcggc ccccactcca tggcgaatcc gtcctggcgg atcgtgcgca ttttcccgcc
     1081 cgggaccgcg tcggcctcca ggagcaggat ctcggcctcc tttcccgcgt cggagaggga
     1141 cttcacgagg tagtgggccg ttgccatccc ggaaaggccg gctccgatga tcacgatacg
     1201 aggcatcaga ccgctcccag aacgagatcc ttcagcgcgg cgatgaaccc gggcgcgtcg
     1261 ttcaaagcgg gcgcccgcag gaacgattgt atacccgctt gccgcgcgtg ggcggccaga
     1321 cgcacgtcca gctcgtgaag cgtttcgatg tgctccgaca cgaagctcac cgggacgacg
     1381 accagcgtct tcaccccctc ccgcccgagt cgcgttacct cggagacggt gtcggggggg
     1441 agccacgccg tcggacccac cttgctctgg tacgagaccc cgtgcggcag gccggggaag
     1501 gagcgcatca ccgccgcgac ggtgcgctcc gtctcctccc ggtacgggtc cccgccgtca
     1561 atcagcgatt gtggaacccc gtgggcgctg aagagaagaa aaatgccgcc gcggtccgct
     1621 tcgcggatca cggcggcgat cgtctcggcg agggcggcga cgtacttcgg gtgttccggg
     1681 taggagcgga tctccgtcaa cgggcaggag caccccgccc acttcatcca gcggcgcagg
     1741 tcggaaaaac tcgatcccgt ggtagccgaa cagaagtgcg ggtagagggg gagggcgatc
     1801 gcccgggcca cgccgtcctc cttcatctcg agggccgcgt gtttcgcgag gggaaaccag
     1861 taccgcatcc ccgtgtacac cttgaagcgc cctcccgtcg cgcgcagcgc ttcctcgagg
     1921 gcgaaccgtt ggcgctccgt gatcgcggcg atgggcgacc ctccgccgat ctcctcgtaa
     1981 tatcggcgga ccttccgggc gcgaagcccc gacacgatcc atgcgaagag cggctgcgtg
     2041 atcggcgccg cgggcagccg gatcagctcc ttgtcggaga agagcctcgc gaggaacggc
     2101 cggacggcgt tcagggagtc gggacccccc atgttgagga ggacgacgcc cgtgaaagga
     2161 ccttcggcgg gcacgcgcgg gcctttcccg gaaacgccgc tcacaccttc cggccgatgc
     2221 ggtggaccgc ctctaccatc gcgatcgcgt gttccgggtt ggtgggcgga aggatcccgt
     2281 gcccgaggtt gaagatgtgg gaggacgcct tctcgccgcg ccgcaggacg tcccgcacgc
     2341 agtcgtcgat cttctccggc gggtggaaga gcatcgtcgg gtccatgttc ccctgcagcg
     2401 ccttgtccgg gccgacgacc atcgccgcca cgtcgagcgg gatccgccag tccacggcca
     2461 cgacgtcgat cggcagggtc ttgatggccg tgagcagcgt ggcgcagtcg ttcgcgaagt
     2521 ggatcacggg gacccccttc cggttcaggc cggagacgac ctggcgggtg tacgggaggg
     2581 cgtactcctc gtagtccccc ggggtgagca cccccgccca cgtgtcgaag atctgcaccg
     2641 cctgcgcgcc cgcggcgatc tgggcgttga ggtacaggat caccgtcttc gcgatcttgt
     2701 tcatcaggga ccggtacacc tccggcgcct ggtacatcag cttcttcagc tggatgaagt
     2761 tcttcgaggt ccctccctcc acgatgtagg aggcgagcgt gaacggggcg cccgagaagc
     2821 cgatcagcgg caccttcccg tcgagctcct tgcgcaggat gcggatcgcc tccatcacga
     2881 aggggacctt ctccgcgggg tcgggcacga gaagggaatc cacgtccttc tgtccccgga
     2941 tcgccttccc cagcatcggc cctttcccgt cgtggaactc gagcggcacc cccatcgcct
     3001 cgacggggat gaggatgtcg gagaagagga tcgccgcatc cacgcccagc cgctcgaccg
     3061 gctggatcgt gacctgcgcg gccagctccg gggtcttgca catcgtgagg aacgaattgt
     3121 tcccgcggat cttctgatac tcggggaggt agcggcccgc ctgccgcatg atccacacgg
     3181 ggatgacgtc gaccggctca cgccggcacg ccttcaggaa tcggtactcc gtcatgtcga
     3241 cctcgggttc ggttatttcg tcgattttcc cgcctggacc gcctcggcca ggcgcttgcg
     3301 ggtcttgatc gggacgtagt tgcagaacgg ctcctcctcg agataatcct cgaggaccgc
     3361 gtcggcccgc gcccggcagc cgccgcagac gttgatgtat tcgcactcgc cgcaccgccc
     3421 cttgtacttc tcgaagtcgc gcagcgactc gaacagctcc gatttgcgcc agatctcccc
     3481 gaacttctgc ttcttcacgt tgcccgccac cacggggaaa tacgagcacg gctgcacgtt
     3541 ccccttcgag tcgatgaagc agatcgactg ggcgcagatg caccccttgc cgccgccggt
     3601 ggagaacgtc agggaccgcg gctggaattt ctccccttcc tccttcatct tctggagacg
     3661 cacccggtag tagtgcggcg cgcaggtggg gcgcacgagc atctccgtct cttccttctc
     3721 catctgatag tgccactcga ggatctcctc gtaatcctcc ttgctgatca actcgctcat
     3781 gatcgcctcc ccccggcccg tgggaacgat catgaacatg taccacgcgt gggcgccgat
     3841 cgccttcgcc agcttgcagg tggccgcgat gtcgtgctgg ttccgctggg tgaaggagga
     3901 gttgacgatg aactgcatcc cgttgcggtt gaagatctcc gccgcccgaa gcgtcgcctc
     3961 gaacgctccc ggctgctttc ggaagtcgtc gtggaccgcc gcggtggacc cgtccagcga
     4021 cagggagcag atccggatcc ccgactcctt catctttccc acgacctcgt ccgtgacgag
     4081 cacgccgttc gtggcgatgc acatccggaa ccccttctcc gtcccgtact tcgcgatctc
     4141 gaagaggtcc ttccgcagaa gcggctcccc gcccgagagg accagtaccg gcttcgagat
     4201 ctccgcgatg tcgtcgatca gctgtttcgc ctccgggagc gaaaaatccg agtcgtggga
     4261 ggccatcgag gaagaggcgc ggcaatggat gcagttcagg ttgcagcggc cggtcacttc
     4321 ccaggcgatc cacttcggga tgaactcttc cttggccaag gtgtatcctt tccgtaagtc
     4381 gtaaacacgc attataaccc gccgggcacc gggaggggac cgatggcgag aaaacgaagg
     4441 aagcggtacc actccccggg gacgcagccc gggacgctcg cggcgcacgc cgaggcgggg
     4501 gacgcgccgg tccgggtcac gtcgttccaa tacgacgcga gccggtgcga ggagcggacc
     4561 gtcgccccct ccgagatccc ttccctgtcc cccccggagg gaggggtgct gtggctcgac
     4621 gtctgcggcc tgtccgatcc ctccgtggtc cgggcgatcg gcgaccgctt ccggatccac
     4681 tccttggctc tcgaggacgt gctgaacgtc ccgcagcgcc caaaggtgga gtggtacggc
     4741 gaccacctcc tggtcgtgct gtccgagatc cggtacccgg atcttcccga acagatggcg
     4801 tttctgctcg cggaccgggt agtcgtctct ttccaggagc gcccgggaga cgcgttcgag
     4861 ccggtcaggg agcgcctgcg gcagggaaag ggacgggtcc gcgccgaggg ggccgattac
     4921 ctcctttact ccctgtgcga cgccgtactg gacgccttct tcccgacgct ggagcatctg
     4981 ggcgacgagg tggaggagat ggaggagcgg gtgctcatct ctcccgtgcc ggaaacgttc
     5041 ctcgccatcc ggcgcctgaa gcgggaactg atggcggtgc gccacgccgt atggcccgcc
     5101 cgggacgcgt tgaacctgct gctgatcgag gagcacgccc tgatccgccc cgggaccaag
     5161 gtgttcttcc gggactgcta cgaccacacg atccagttga tggacatggt ggagacgttc
     5221 cgggaaatgg cgtcggggct ggtggacgaa tac
//
LOCUS       DASVJL010000392         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_627480, whole genome shotgun sequence.
ACCESSION   DASVJL010000392 DASVJL010000000
VERSION     DASVJL010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627480"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..2156)
                     /gene="selB"
                     /locus_tag="VF357_08280"
     CDS             complement(234..2156)
                     /gene="selB"
                     /locus_tag="VF357_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX7524185.1"
                     /translation="MSKTVILGTAGHIDHGKSSLVRALTGTDPDRLKEEKERGITIEL
                     GFARLSLPSGTLAGIVDVPGHERFVRTMVSGAAGVDIVLLVIAADEGVMPQTREHLDI
                     CRLLAVRHGIVVLNKCDKAESEWMDLQEEEIHTLVRGTFLEGAPIVRVSAATGEGLPE
                     LVSALDRIAAGISGKDASDLFRLPVDRSFSMKGFGTVVTGTVIGGTVATGEEVAILPG
                     GTVAKVRGLQVHGGPVERSSAGTRTAVNLQGVEKESAPRGSVLCHPGTFSPTKSAEVF
                     VEYLPLVPKPLRHRGQVSFHAGTFSCVGRILLYGQAEIPPGGSGYGRVLLSEATVLSG
                     GDRFILRGFSPLANFGYTVGGGAVLHPKPPARRGAGKAVPEALPRLRSEDPAERVLAT
                     VEDVGAAGVTPADAAVVAGLGPERTRGALKELAAKGMITEVPASGKLWHRSAISAASS
                     FCGQALAKLHDRHPERDGFPREEIASLFPIPPDPGFLSLALEGNASISRQGELHFLPA
                     RKPKAVEPGSPLARKVAEFIRAAGDTAPGRAELLEAVKGVSADPKAVEKVVDALARAG
                     EIVRVKELLFDGAALRGIQEKLVAFLAKRGEVAVPEFKEMTGLSRKYIIPLLEHFDAQ
                     KITLRVGDKRVLRKGK"
     gene            2229..>2454
                     /locus_tag="VF357_08285"
     CDS             2229..>2454
                     /locus_tag="VF357_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX7524186.1"
                     /translation="MTRPDPPGEPPPHLVPVEESIDLHAFLPRDVVSVVEEYLEAAAR
                     KRYREVRLIHGKGTGAQRAAVRALLARHPLV"
BASE COUNT          386 a          874 c          810 g          384 t
ORIGIN      
        1 gccaaattca ctctgcccgc gacgtccctc ctggattacg actcgcgcgc caagaatttc
       61 ggcagctggg tctaccactg tcacatcctc gagcacgagg aaaacgacat gatgcggccg
      121 ttcgaagtcg tgccttagaa cgaacggcat agcaggacgg ccggtgcaag catcgacggg
      181 ggggccgcct tcgggcggcc ctctcctttt ttccattacc gccggcccgg cctctacttc
      241 cccttccgaa gcacccgttt gtcgcccacc cgcagggtga tcttctgcgc gtcgaaatgc
      301 tccagcagcg ggatgatgta tttccgggac agccccgtca tctccttgaa ctccggcacg
      361 gcgacctcgc cgcgcttcgc gagaaacgcg acgagcttct cctggatccc gcgcagcgcc
      421 gccccgtcga agagcagctc cttgacacgg acgatctccc cggcgcgggc gagggcgtcg
      481 accaccttct ccaccgcctt cgggtccgcc gaaacgcctt tgaccgcctc gaggagctcg
      541 gcgcggcccg gcgcggtgtc gcccgccgcg cggatgaact ccgcgacctt gcgggccagc
      601 gggctccccg gttccaccgc cttcggcttg cgggccggga ggaagtggag ctctccctgg
      661 cgggagatgg aggcgtttcc ctccagggcg agggagagga accccggatc gggggggatg
      721 gggaacaggg aggcgatctc ttcccgcggg aacccgtccc gttccggatg gcggtcgtgc
      781 agcttcgcaa gggcctggcc gcagaaggac gacgcagcgg aaattgctga acggtgccat
      841 aattttccag atgcggggac ttcggtgatc atccccttgg cagcgagctc cttcaaggct
      901 ccgcgggtcc gctccggccc aagcccggcg acgaccgccg cgtccgcggg agtaacgccc
      961 gccgcgccga cgtcctccac cgtggccagg acgcgctctg cggggtcttc cgaacgcaac
     1021 cggggaagag cctccgggac cgccttcccg gcccccctcc gggcgggcgg tttcggatgc
     1081 agcacggcgc cgccgccgac ggtgtagccg aagttcgcca gcggggagaa accccgcagg
     1141 atgaaccggt cgccgcccga gaggaccgtc gcctcggaga ggagcacccg gccgtacccc
     1201 gagcctcccg gcgggatttc ggcctgcccg tagaggagga tcctcccgac gcaggagaag
     1261 gtccccgcgt ggaaggagac ctggccccgg tggcgcagcg gcttggggac cagcggaagg
     1321 tactcgacga agacttccgc ggatttcgtg ggggagaacg tcccgggatg acacaggacc
     1381 gacccgcgcg gggcgctctc cttctccacc ccctgcaggt tgaccgcggt gcgggtcccc
     1441 gcggacgatc gttccaccgg gccgccgtga acctgcagcc cgcgcacctt cgcgaccgta
     1501 cccccgggga ggatcgccac ctcctcgccc gttgcgaccg tccccccgat caccgttccc
     1561 gtgaccacgg tcccgaaccc cttcatcgag aaggagcggt ccacggggag gcggaacagg
     1621 tccgacgcgt ccttcccgga aattccggcg gcgatccggt cgagggcgga aaccagttcg
     1681 gggagcccct cgccggtggc ggcggaaacc cggacgatcg gtgccccctc gaggaacgtt
     1741 ccccggacaa gcgtgtggat ctcctcctct tggagatcca tccactcgct ctccgctttg
     1801 tcgcacttgt tgaggacgac gatcccgtga cgcaccgcga gaaggcggca gatgtcgaga
     1861 tgttcgcgcg tttgcggcat caccccctcg tccgcggcga tgacgagcag gacgatgtcg
     1921 acccccgccg ccccggatac catcgtccgg acgaaccgct cgtgccccgg cacgtcgacg
     1981 atgccggcga gcgttcccga cgggagggag aggcgggcga acccgagctc gatcgtgatc
     2041 ccccgctcct tctcctcttt cagccggtcg gggtcggtgc cggtcagcgc gcgcacgagg
     2101 gaactcttcc cgtggtcgat gtgcccggcc gtcccgagga tcacggtttt cgacatggga
     2161 gatatcttat cgtaaccgat cggttgcggt accatgccga tcatgggcga cgacaagctc
     2221 tacctctcgt gacgcggccg gatccccccg gggaaccgcc cccgcacctc gtgccggtgg
     2281 aggagagcat cgacctccac gccttcttgc cgcgggacgt cgtctccgtc gtggaagagt
     2341 atctcgaagc ggccgcgcgg aaacggtacc gggaggtgcg gctgatccac gggaagggaa
     2401 cgggagctca gcgcgccgcg gtccgcgcgc ttctcgcgcg ccatccgctc gtcg
//
LOCUS       DASVJL010000393         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_627746, whole genome shotgun sequence.
ACCESSION   DASVJL010000393 DASVJL010000000
VERSION     DASVJL010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627746"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VF357_08290"
     CDS             complement(<1..278)
                     /locus_tag="VF357_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524187.1"
                     /translation="MIHPTAIIDPGAVLGNDVTVGPFAVIGPKVTIGDGTTVGSHAVI
                     ESHVRMGKGNRIHSFASVGAIPQDLKFRGEESWVEMGDGNIVREFATV"
     gene            complement(275..727)
                     /gene="fabZ"
                     /locus_tag="VF357_08295"
     CDS             complement(275..727)
                     /gene="fabZ"
                     /locus_tag="VF357_08295"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX7524188.1"
                     /translation="MFRIQEIMELLPHRYPFLLVDRIVEWDPGKRIVGIKNVTINEPF
                     FQGHFPGHPIMPGVLIVEALAQTGGILALKAMGGEKRIAYFTGIDNCKFRRPVVPGDQ
                     IRLEINVIAHKGPVWKMHGEARVDGAIAAKGDVTATIPDGVETGGEGE"
     gene            complement(729..1766)
                     /gene="lpxD"
                     /locus_tag="VF357_08300"
     CDS             complement(729..1766)
                     /gene="lpxD"
                     /locus_tag="VF357_08300"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX7524189.1"
                     /translation="MAVGSKLLLSVLAGEIGARLEGTDAEVSGIAGVDEAGPGQVTFL
                     SNPKYARQARETKASAIIVKQPISGAGCAFLLTPDPYLAFARAVERFHPPVRLAAGVS
                     AQASVHPTAALGKEVHISPFAVVAEGAVVGDRVTLYPGAYVGKGAIVGEDTVLHPGVT
                     LYEGVRVGKRVLLHAGCVIGSDGFGFAPTPQGYRKIPQVGTVEIGDDVEIGANTTIDR
                     AALGVTRVGPGTKLDNLIQVGHNVEIGRDTVIAAQVGISGSARIGNRVMIGGQSGLAG
                     HLEVGDGVMLGAKTGVAVSLTASENRAWSGVPAMPHRTWLKMVTLLPRLPELFRRVTR
                     LEGGKPAEREE"
     gene            complement(1750..2358)
                     /locus_tag="VF357_08305"
     CDS             complement(1750..2358)
                     /locus_tag="VF357_08305"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX7524190.1"
                     /translation="MRKAGIILVAAMVTAIFAGTSLAEGLRVAVIDMNKILNESEAGL
                     AAKKKIEARYEELKKKIDVTQEEARKIKEEIDKQKVMLGKEKLKEKEDALQAKVNELR
                     QMTQEGEQEMQARQTESTREVLKTVEAKIAVVVKADKLDLVLEKSAGVIHSSPSMDIS
                     SRVLALVNEGGKAAPEKKAAPEKKAPPEKKAAPEKKGGGSGK"
     gene            complement(2391..>2989)
                     /locus_tag="VF357_08310"
     CDS             complement(2391..>2989)
                     /locus_tag="VF357_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX7524191.1"
                     /translation="GWNVTRNTTDKFIDPSKGGVQSASLEYAGGPLGGDSQFVKYFLN
                     AKWFYPVTATTVFSWNLLWGHVIPTAGGAEVPLFERFFLGGPYSIRGFRSRELSPRDP
                     NTGESIGGNKELVGNLEYLFPLVADIGFKGVLFFDVGNAWAQGSWPFNDQGLWASYGV
                     GIRWYSPMGPLRFECGWNLDRPQGQPKRVMEFTIGTAF"
BASE COUNT          449 a         1120 c          846 g          574 t
ORIGIN      
        1 ttcacggtgg cgaactcgcg gacgatgttc ccgtccccca tctccaccca cgactcctcg
       61 ccgcggaact tcaggtcctg cggaatcgca ccgacggagg cgaacgagtg gatgcggttc
      121 cccttcccca tccggacgtg ggattcgatg accgcgtgcg atccgacggt ggtcccgtcg
      181 ccgatcgtca ccttcggccc gatcacggcg aacggcccca ccgtgacgtc gttcccgagc
      241 accgcccccg ggtcgatgat ggcggtcgga tgaatcattc cccttccccg cccgtctcga
      301 cgccgtccgg gatcgtcgcc gtcacgtccc ccttcgccgc gatggcgccg tcgacccggg
      361 cctccccgtg catcttccag accggcccct tgtgggcgat cacattgatc tcgagccgga
      421 tctggtcccc cggtacgacg gggcggcgga acttgcaatt gtcgatcccc gtgaagtagg
      481 cgatccgctt ctccccgccc atcgccttga gcgcgaggat cccccccgtc tgcgcgaggg
      541 cttccacgat caggaccccc ggcatgatcg ggtgcccggg aaagtgcccc tggaagaacg
      601 gctcgttgat cgtcacgttc ttgatgccga cgatccgctt ccccggatcc cactccacga
      661 tccggtccac gagcagaaac gggtaacggt gcggcaacaa ctccatgatt tcctggatcc
      721 ggaacacgtc actcctccct ttccgcgggc tttccgcctt ccagccgcgt gacccgccgg
      781 aagagctccg gcagtctcgg caggagcgtc accattttca gccatgtcct gtggggcatc
      841 gcgggcactc ccgaccacgc ccggttctcg ctcgccgtga gcgagacggc cacaccggtc
      901 ttcgcgccga gcatcacccc gtcgcccacc tcgaggtgcc ccgccaaccc cgactgcccg
      961 ccgatcatca cgcggttccc gatccgggcg ctgcccgaga tccccacctg ggcggcgatc
     1021 accgtgtccc ggccgatctc cacgttgtgc cccacctgga tcaggttgtc cagcttggtc
     1081 ccgggaccga cgcgggttac cccgagcgcc gcgcggtcga tcgtcgtgtt cgccccgatc
     1141 tctacgtcgt ccccgatctc gacggtgccc acctgcggga tcttccggta tccctgcggc
     1201 gtcggcgcga acccgaaacc gtcgctgccg atcacgcagc cggcatgcag gagcacccgc
     1261 ttcccgacac gcacgccttc gtagagggtg acccccgggt gaagaacggt gtcctcaccg
     1321 acgatggccc ccttcccgac gtacgccccg gggtacagcg tgacccggtc tccgacgacc
     1381 gctccctcgg cgacgacggc gaaggggctg atgtggacct ccttccccag cgcggcggtc
     1441 ggatggacgc tggcctgtgc ggagacgccc gcggcaagcc gcaccggcgg gtggaaccgc
     1501 tcgacggcgc gggcgaacgc gaggtacggg tccggcgtca gcaggaacgc gcaccctgcg
     1561 ccggagatcg gctgtttgac gatgatcgcg gacgccttcg tctcgcgggc ctgacgggcg
     1621 tacttcgggt tcgacaggaa ggtgacctgc cccggcccgg cctcgtccac cccggcgatc
     1681 ccgctcacct cggcgtccgt accctcgagg cgagccccga tctcccccgc caggaccgag
     1741 agaaggagtt tacttcccac tgccacctcc ctttttctcc ggcgccgcct ttttctccgg
     1801 cggcgccttt ttctccggcg ccgccttttt ctccggcgcc gccttgccgc cctcgttcac
     1861 cagggccagg acccgcgacg agatatccat cgacgggctg gaatggatca cccccgccga
     1921 cttctcgagg acgagatcga gcttgtcggc ctttacgacg accgcgatct tcgcctcgac
     1981 ggtcttgagc acttcgcggg tggactcggt ctgccgggcc tgcatctcct gctccccctc
     2041 ctgggtcatc tggcgcagct cgttgacctt cgcctggaga gcgtcctctt tctccttgag
     2101 cttctccttg cccagcatca ccttctgctt gtcgatctct tccttgatct tgcgtgcctc
     2161 ttcctgcgtc acgtcgatct ttttcttcaa ctcctcgtac cgcgcttcaa tcttcttctt
     2221 cgccgccagg cccgcctcgg attcgttgag gatcttgttc atgtcgatga cggccacccg
     2281 cagcccttcc gcgagggagg tccccgcgaa gatcgccgtg accattgccg ctaccaggat
     2341 gattcccgct ttccgcatct cttgtctcct tttcccttcc cgtagttcga tcagaacgcc
     2401 gtcccgatcg tgaattccat cacacgcttc ggctgcccct gcgggcggtc gagattccat
     2461 ccgcactcga agcgcagcgg ccccatcgga gagtaccagc gtatgcccac cccgtaggag
     2521 gcccacagtc cctggtcatt gaagggccag gatccctgcg cccatgcgtt ccccacatcg
     2581 aaaaacagca cccccttgaa tccgatatcg gcgacgaggg gaaacaggta ctccaggttc
     2641 ccgaccagtt ccttgttccc cccgatcgat tccccggtgt tgggatccct cggggaaagt
     2701 tccctggagc ggaacccgcg gatgctgtac ggccccccca ggaaaaaccg ttcgaacagc
     2761 gggacctccg cgcccccggc cgtcggaatc acgtgtcccc agaggaggtt ccacgaaaaa
     2821 acggtggtag cggttaccgg gtagaaccac ttcgcgttca ggaaatattt cacgaactgg
     2881 ctatcgccgc ccaacggccc gcccgcgtac tccagactgg ccgattggac gcctcccttg
     2941 gacgggtcga tgaacttgtc cgtcgtgttc cgcgtcacgt tccaaccga
//
LOCUS       DASVJL010000394         3958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_253840, whole genome shotgun sequence.
ACCESSION   DASVJL010000394 DASVJL010000000
VERSION     DASVJL010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253840"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VF357_08315"
     CDS             <1..606
                     /locus_tag="VF357_08315"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7524192.1"
                     /translation="LSFDYDPDSVACTREVKRRYFPEDGRWRILEGSVLNKDFLASLG
                     RFDIVFSWGVLHHTGSMWQAIDNTLSLIKPGGLLAIAIYNDQGWKSTSWRRIKRLYCG
                     APRVLRPAFFLPAFFFYELKGMAARGLHGEGLFASWRGRGPRGMSRLHDWIDWMGGYP
                     FEVAQPEEVSAFLRDRGLHPVKFVACTGSGNNEFVFRSPEK"
     gene            620..1774
                     /locus_tag="VF357_08320"
     CDS             620..1774
                     /locus_tag="VF357_08320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7524193.1"
                     /translation="MKIFYVLPNIENGGAERQVLTLARQLDRSRFSPSLVTTAGGGSL
                     YESFRAILPVSVIGDPRQARPRPPRNPFVHLRGILEMGGIFRERSPDIVHAYLPAANV
                     IGPIATRLSADSRVIVSKRSLANYKEGHPLLRRVESLGNRLADAILVNSDAVRRDVER
                     SERHWEGKFRKIYNGVAQIPAWTPEEIASFRAREGLPPRAPVTVCVSNFFEYKGHDDL
                     VAAVARVAEDFPDATFVLIGRDAGSLEAIRELAGDLGILPRLRFAGPRTDVPDFLRAA
                     DLFVHPSHQEGFSNAILEAMAAGLPVVACDVGGNPEAVVEGETGRLVPPRCPGRLAEA
                     MTELLRDEGKRRAMGEAGRHRAEERFSLGRMVAEVEAMYESLVPRQGGGR"
     gene            1771..2805
                     /locus_tag="VF357_08325"
     CDS             1771..2805
                     /locus_tag="VF357_08325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7524194.1"
                     /translation="MSGGEIRPYYGDKLGSLRDIFGAAEVRAEERRIVVDGREYPVVD
                     DVIILLPPGQWPAGVRIRLSGAAAWAGKTPEDGNAGDIQFTFGEEWQCFPEILPEHKK
                     EFQQYFDLMDIGSLANARICDLGCGIGRWSHFLKDLCREMVLLDFSEAIFVARENLRG
                     AGNALFFLGDLRRMPFRDDFADFLFTLGVLHHLPTGALEEVRALSRRAPTLLVFLYYA
                     LDNRPAFWRGLLSAVTAVRLALSRTEGPRFRAALTWALTICVYLPLISLGLAARPLGL
                     ASKVPLYDFYRGKSLRRIRQDVYDRFFTRIEQRFRKEEILALRDTFRQVVVSDDPPYW
                     HFLCRREPIR"
     gene            2837..>3958
                     /gene="asnB"
                     /locus_tag="VF357_08330"
     CDS             2837..>3958
                     /gene="asnB"
                     /locus_tag="VF357_08330"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX7524195.1"
                     /translation="MCGIAGFLSTGTARPGERVLRAMGDALAHRGPDASGTHLSPDGR
                     VGLSHRRLSILDLSPAGAQPMFSADGSLVLSFNGEVYNFREIRAELEGKGHTFRGGSD
                     TEVMLAAFREWGVDAAVRRFIGMFAFALWDSGTRKLYLVRDRLGIKPLYLARLPGMLL
                     FASELKALLAYPDFPRELNRSALQYFLEFQYVPGPHAIYQDVEKVLPGHIVEIGQQGE
                     ITDHHYWDLFDHWGKAEGRPRTEGEYVEELSALLDSSIRYRMISDVPLGAFLSGGIDS
                     SLVVALMQKTASGPVKTFSIGFREGGYDESPYARAVAAHLGTDHHEKICTPQEAQALV
                     RKIPDAYDEPFADSSAIPTMLVSEFTRQHVTVSLSGDGGD"
BASE COUNT          636 a         1254 c         1357 g          711 t
ORIGIN      
        1 ctctccttcg attacgaccc cgactccgtt gcgtgcaccc gcgaagtgaa gcgaagatat
       61 tttcccgaag acggacgttg gcggatcctg gagggttccg ttctcaacaa ggatttcctc
      121 gcctcactgg gacggttcga catcgtcttc tcctggggtg ttctccacca cacggggtcc
      181 atgtggcaag ccatcgataa caccctctcc ctgatcaagc cgggtggcct cctcgccatc
      241 gcgatctaca atgatcaggg gtggaagagc acgtcctggc gcaggatcaa gcgtctctac
      301 tgcggggccc cccgggtgct gcgcccggca tttttcctcc ccgcattctt tttctacgaa
      361 ctgaagggga tggccgcgcg ggggttgcac ggggaaggtt tgttcgcgtc gtggcgaggg
      421 cgcggaccga gggggatgag ccgcctgcac gactggatcg actggatggg cgggtaccct
      481 ttcgaggtcg cgcagccgga agaggtgtcg gcgtttctca gggacagagg cctccacccg
      541 gtgaaattcg tcgcctgcac gggatcgggg aacaacgagt tcgtgttccg gtctccggaa
      601 aagtgagcgg gggacgccgt tgaaaatctt ctacgtcctt ccaaacatag agaacggtgg
      661 ggctgaaaga caggtgctta cgctcgcccg tcaactcgac cggtcccgat tctccccgtc
      721 cctcgtgacc acggcggggg gcgggtccct gtacgagtcg ttccgcgcga tccttccggt
      781 ctcggtcatc ggcgacccgc ggcaggcgag acccaggccg ccccggaacc ccttcgtgca
      841 ccttcgggga atcctcgaga tgggcggcat tttccgggaa cgatctcccg acatcgtgca
      901 cgcctatctc cccgccgcga acgtcatcgg gcccatcgcg acgcgcctgt cggccgattc
      961 ccgggtcatc gtcagcaaac ggtcgctggc caactacaag gaaggacacc cgctgctccg
     1021 gagggtcgag tccctgggga accgacttgc cgacgccatc ctggtaaatt cggacgccgt
     1081 ccgcagggac gtcgagcgga gcgagcggca ctgggaaggg aagttccgga agatctacaa
     1141 cggggtggcg cagattcccg cgtggacgcc ggaggagatc gcgtccttcc gtgcgcgcga
     1201 ggggctgccc ccacgggctc ccgtgacggt gtgcgtctcc aacttcttcg agtacaaggg
     1261 gcacgacgat ctcgtggccg cggtggcgag ggtcgcggaa gatttccccg atgcgacctt
     1321 cgtgctcatc gggcgggatg cgggctccct cgaggccatc cgggaactgg cgggggatct
     1381 cgggatcctt ccgcgcctcc gtttcgccgg gccccgcacc gatgttccgg acttccttcg
     1441 ggcggcggac cttttcgtgc acccctcgca tcaggagggt ttttccaacg cgatcctcga
     1501 ggcaatggcc gcagggctgc cggtggtcgc gtgcgacgtg gggggaaacc cagaggcggt
     1561 cgtggagggc gagacgggtc ggctcgtgcc gccccggtgc cccgggcggc tcgcggaggc
     1621 gatgacggag ctcctgcggg acgaggggaa gcgcagggcg atgggggagg cagggaggca
     1681 ccgcgccgaa gagcggttct cccttggacg gatggtcgcc gaggtcgagg cgatgtacga
     1741 atcgctggtg cctcgccaag gagggggcag gtgagcggcg gggagatccg gccttattac
     1801 ggggacaagc tggggtcgct ccgggacatc ttcggcgctg cagaggtccg cgccgaggag
     1861 cgtcgaatcg tggtcgacgg tagggagtac ccggtggtcg acgacgtcat catccttctc
     1921 ccgccgggac agtggcccgc cggggtgcgg attcggctat ccggggcggc cgcctgggcg
     1981 gggaagacgc cggaagacgg caatgccggg gacatccagt tcaccttcgg ggaggagtgg
     2041 cagtgcttcc cggagatcct cccggagcat aagaaggagt tccaacagta cttcgacctc
     2101 atggacatcg gttctctcgc gaacgcgcgg atatgcgacc tgggttgcgg catcggacgc
     2161 tggagtcact ttttgaaaga cctgtgccgg gagatggtcc tcctggactt ttcggaggcg
     2221 atcttcgtcg cccgggaaaa cctgcgcggg gcgggcaacg ccctcttctt cctcggagac
     2281 cttcggcgga tgccgttccg cgacgacttc gccgacttcc tgttcacgtt gggagtgctc
     2341 caccacttgc ccaccggcgc gctggaagag gtccgggcgc tctcccggcg cgcgcccact
     2401 ctcctcgtct tcctgtacta cgccctggac aaccggccgg cgttctggcg tgggctgctc
     2461 tccgccgtaa cggcggtccg actcgccctg tcccggacgg aggggccgcg gttccgggcg
     2521 gcgctgacct gggcgttgac gatctgcgtc tacctgcccc tgatctccct aggactcgcc
     2581 gcgcgccccc tcgggttggc gagtaaggtt ccgttgtacg atttctaccg gggaaaatcg
     2641 cttcggagga tccgccagga cgtgtacgat cgattcttca cgcgaatcga gcaacggttc
     2701 cggaaagagg agatcctcgc acttcgagac acgttccgcc aggtcgttgt ttccgacgat
     2761 ccgccctact ggcacttcct ttgccgcagg gaaccgatcc gatgaatgcg tccgctcgca
     2821 acggagggaa ccggccgtgt gcgggatagc cggattcctt tccaccggaa ccgcaaggcc
     2881 aggcgagaga gtgctgcgcg cgatggggga tgcgttggcg caccgcgggc cggacgcttc
     2941 cgggacgcat ctctcccccg acggccgggt aggcctttcc caccggcggc tgtcgatcct
     3001 cgacctctcc cccgccgggg cgcagccgat gttctccgcg gacgggtcgc tcgtcctctc
     3061 cttcaacggg gaggtgtaca acttccggga gatccgcgcg gagctcgagg ggaagggcca
     3121 caccttccgt ggagggtcag atacggaggt gatgctggcg gcgtttcgcg agtggggcgt
     3181 agacgccgcg gtccgccggt tcatcggcat gttcgccttc gcgctgtggg attcaggaac
     3241 gcggaagctg taccttgtcc gcgatcggct cgggatcaag ccgctctacc ttgcgcgcct
     3301 gccggggatg ctgctcttcg cctccgaact gaaagcgctt ctcgcgtacc cggacttccc
     3361 gcgggagttg aaccggtccg ccctccagta cttcctcgag tttcagtacg tcccggggcc
     3421 gcacgcgatc taccaggacg tagagaaggt gctgccgggg cacatcgtgg agatcgggca
     3481 gcaaggggag atcacggacc accactactg ggacctgttc gatcactggg ggaaagcgga
     3541 aggccgcccg cggacggagg gggagtacgt ggaggaactc tccgcactgc tcgactcctc
     3601 catccggtac cggatgatca gcgacgtgcc gctgggggcg ttcctctccg gggggatcga
     3661 ctcctccctc gtcgtggcgc tgatgcaaaa aacagcgtcg ggaccggtga agacgttctc
     3721 catcggcttc cgggagggcg gatacgacga gtcgccgtac gcgcgggcgg tggcggcgca
     3781 cctcgggacg gaccaccacg agaagatctg caccccccag gaggcgcaag cgctggtccg
     3841 gaaaatcccc gatgcgtacg acgagccgtt cgccgactcg tcggccatcc cgacgatgct
     3901 ggtgtcggaa ttcacgcggc aacacgtcac cgtcagcctg tcgggggacg gaggggac
//
LOCUS       DASVJL010000395         7074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_347275, whole genome shotgun sequence.
ACCESSION   DASVJL010000395 DASVJL010000000
VERSION     DASVJL010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347275"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VF357_08335"
     CDS             complement(<1..307)
                     /locus_tag="VF357_08335"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524196.1"
                     /translation="MTAPYPIQYVKGVGPRLGEKLAARGIRTPHDALYFFPKDYEDRR
                     KVVPIRELKAGMTVPVRGKILSVQGGGKGFRPAQVLEVVIADGTGRLSAKWFRFHPSL
                     "
     gene            complement(334..3606)
                     /gene="carB"
                     /locus_tag="VF357_08340"
     CDS             complement(334..3606)
                     /gene="carB"
                     /locus_tag="VF357_08340"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX7524197.1"
                     /translation="MPKRNDLKKIMLIGSGPIIIGQACEFAYSGTQACKALREEGYEV
                     VLVNSNPATIMTDTDFADRTYIEPITPEIVGKIIERERPDALLPTIGGQTGLNIAVSL
                     HEMGILQKFGVELIGASFEAIQKAEDRNLFRKAMEKLGLIVPRSGYIRSLEEALGVIP
                     DIGYPAIIRPSFTLGGTGAGIAYNREEFEEGIRWALDASPKHTALVEQSVIGWKEFEL
                     EVMRDLADNVVIVCSIENVDPMGVHTGDSITVAPAQTLTDKEYQIMRNAALRIIREIG
                     VDTGGSNIQFAVHPETGEMVIIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IRNDITRETPASFEPTIDYVVTKIPRFTFEKLPQTEDVLGTQMKSVGEVMAIGRTFKE
                     SLQKAIRSLENSVYGFEEMMPPATPPNPAARRKTITEKLRKPNSLRLYYIGEAFRDGW
                     TVEEIRESTGIDPWFLENIRQIIAMEGEIRARAEAFRKEAATQPAAFASFLRRMKGNG
                     FSDRRLSKLLGVGEDDVRKIRYAAGVRAVFKRVDTCGAEFEAHTPYLYSTYECENEAK
                     PTDRKKVVILGGGPNRIGQGIEFDYCCVHGVFALREEGFETIMVNCNPETVSTDYDSS
                     DRLYFEPLTKEDVLAIIEEEKPVGVIVQFGGQTPLKLAVPLEKEGVRILGTSPESIDR
                     AEDRERFAEMLNLLGLSQPPNGIARSTPDAVATAARIGYPVLLRPSYVLGGRAMEIVD
                     DEEGLRRYLTEAVSASEAKPVLVDRFLEDAIEIDVDAISDGETVVVGGIMEHIEEAGV
                     HSGDSACSLPPHSISTETIAEITRQTKALAAELSVIGLMNVQFAIQRGKIFILEVNPR
                     ASRTIPFVSKAIGVPLAKLAAKVMAGRKLADLGFTSEVVPGHVCVKEAVFPFIKFPDV
                     DTLLGPEMKSTGEVMGIDRTFGTAFAKAQIGAGMILPRSGKVFLSVRDEDKEGVLAAA
                     ENLNLCGFSLIATRGTAAFLTARGLPCDTVRKVNEGRPHVADLIRNGEIALVINTPLG
                     AQSKADSYYIRRASLVYNVPYFTTLAAARAVSLAIPGLIQDELSVRSLQEYHGTTRPT
                     GR"
     gene            complement(3606..4409)
                     /locus_tag="VF357_08345"
     CDS             complement(3606..4409)
                     /locus_tag="VF357_08345"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7524198.1"
                     /translation="MNRLEGERGFCGAGRLARVAAVSVHPGEEPPISGTRGSGTIFFS
                     HCNMKCLFCQNYPISQYGNGREMDTETLAGEMLRLREKEVHNVNFVTPTSHVPQMLEA
                     VLLARGMGFDLPVVYNTNGYDALETLALLDGVVDIYLPDAKYLNAELADTASGTPDYA
                     RHNEAAIDEMMRQVGFLSLGDDGIAARGVLVRHLVLPGRVGETEAVLAHLSERHGPDL
                     PLSLMGQYFPAWRAAEVEGFDRKVTRKEYDRAIEAAERLGFRNVFIQER"
     gene            complement(4703..5851)
                     /gene="carA"
                     /locus_tag="VF357_08350"
     CDS             complement(4703..5851)
                     /gene="carA"
                     /locus_tag="VF357_08350"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012053752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX7524199.1"
                     /translation="MPDRKAFLVLADGTAYEGTSFGYEGECSGEVVFNTGMTGYQEVL
                     TDPSYKGQIVTMTYPEIGNTGINPEDVESNRPWVEGFIVREAWGPPSNWRHVESLDAY
                     LRRYHVCGIAGIDTRALTRRLRDGGSQMAVLSATDLDADRLASKARELPSLVGRDLVK
                     EVTSDHAVHWTTGDWDLEKGYLPAAAFLGKFGRVPRIVAIDFGIKQNILRMMVSHGFD
                     VTVVPAGVTAAEVLAHSPDGVFLSNGPGDPEGVPYAVATVRALLGNVPMFGICLGHQI
                     MGLALGGKTFKLKFGHHGCNQPVKDLSTGKVEITSQNHNYSVDADSLGSAPRITHVNL
                     NDGTVEGLSVPSKRCFSVQYHPEASPGPHDSRYLFTRFHRYLCGEEEL"
     gene            complement(5844..>7074)
                     /locus_tag="VF357_08355"
     CDS             complement(5844..>7074)
                     /locus_tag="VF357_08355"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX7524200.1"
                     /translation="EDGKVREFLAGEAPARFQGKVISAEGKWVVPGLIDMHVHLRDPG
                     YEWKEDILTGTRAAAAGGFTSVVCMANTNPVNDSPEVTRHIIEKARREGCANVFPVAA
                     VTLGLEGKEMADFSELADAGAVAFSDDGKPVENSLLLRRAMEYVRPFGYRILSHAEDV
                     VLAGGGAAHEGWTARKLGIPGIPSAAEEVAIARDILIARQTGGKLHIQHISTRMGVDL
                     LRMAHRAGLDVTGETAPQYFTLTDAALEGYDTNAKMNPPLRGEEDRMAILEGIQDGTF
                     DAIACDHAPHESYVKKCEFTAAANGIIGLQTSLPLSLSLLAGGKVAPARIVDLLSAGP
                     ARILGLRRKGTLAAGADADVTVIDPDAEWEFAPGDVLSKSRNSPFFGWKMRGRSVATI
                     CGGRVRHATIAGVTADA"
BASE COUNT         1156 a         2339 c         2302 g         1277 t
ORIGIN      
        1 cgagggaggg atggaagcgg aaccatttcg cggagaggcg ccccgtcccg tcggcgatca
       61 cgacctcgag gacctgggcg gggcggaacc cctttcctcc cccctgcacg gaaaggatct
      121 tcccgcggac gggtacggtc atccccgcct tgagttcccg gatgggaacc accttgcgcc
      181 ggtcttcgta atccttcggg aagaagtaga gggcgtcgtg cggtgtccgg atcccccggg
      241 cggcaagctt ttccccaagg cggggcccca cccccttgac gtactggatg gggtacggtg
      301 cggtcaccgc gctcttgggg cggttttgcg gaatcatcga cctgttggcc gtgtcgtccc
      361 gtgatactcc tggagactcc ggacggacag ttcatcctgg atcaggcccg ggatcgcgag
      421 ggagacggcg cgcgcggcgg ccagggtggt gaagtacggg acgttgtaga ccagggatgc
      481 gcgacggatg tagtaggagt cggccttcga ctgggccccg agcggcgtgt tgatgacgag
      541 ggcgatctcc ccgttcctga tgaggtccgc cacgtgtggg cgtccttcgt tcaccttccg
      601 caccgtatcg catgggagcc cgcgcgccgt caggaacgct gccgtgccgc gggtggcgat
      661 gagggagaag ccgcagagat tcaggttttc cgccgcggcg agaaccccct ccttgtcctc
      721 gtcgcgcacg ctcaagaaaa ccttcccgga acgcggcagg atcatcccgg cgccgatctg
      781 cgccttggcg aaggcggtgc cgaacgtcct gtcgatcccc atcacctcgc cggtcgactt
      841 catttccgga ccgagcagcg tgtcgacgtc ggggaacttg atgaagggga agaccgcctc
      901 cttgacgcag acgtggcccg gcacgacctc cgaagtgaac ccgaggtccg ccagttttct
      961 gcccgccatc accttcgcgg cgagcttggc cagcggcaca ccgatcgcct tgctgacgaa
     1021 cgggatagtg cgggaagccc gcggattgac ctcgagaatg aagatcttgc cgcgctgtat
     1081 ggcgaactgg acgttcatca gcccgatgac ggagagttcg gcagccagcg ccttcgtctg
     1141 ccgggtgatt tcggcgatcg tctccgttga gatcgagtgc gggggaaggg aacatgccga
     1201 atccccggag tgtacgcccg cttcctcgat atgctccatg atcccaccga caacgaccgt
     1261 ttccccgtcg gagatcgcgt ccacgtcgat ctcgatagcg tcctcgagga accggtcgac
     1321 gagaacgggt ttcgcctcgg aagcggagac ggcctccgtg aggtacctgc gaagaccttc
     1381 ctcgtcgtcg acgatctcca tcgcccgccc gccgaggacg taggacggac gaagcaggac
     1441 ggggtaaccg atccgggcgg cggtcgcgac cgcatcgggc gtggaccgcg cgatcccgtt
     1501 cggcggctgc gaaagcccca gcaggttcag catctcggcg aaccgttctc gatcttccgc
     1561 ccggtcgatg ctctccggcg aggtcccgag gatccggact ccttcttttt cgagggggac
     1621 ggcgagcttc agcggggtct ggccgccgaa ctggacgatt acgccgaccg gcttctcctc
     1681 ctcgatgatg gcgaggacgt cctctttcgt caggggctcg aagtagaggc ggtccgagga
     1741 gtcgtagtcg gtggagacgg tctcggggtt gcagttcacc atgatcgtct cgaacccttc
     1801 ctcgcgcagg gcgaagactc cgtggacgca gcagtagtcg aactcgatcc cctggccgat
     1861 ccggttcggg cccccgccca ggatcaccac cttcttccgg tcggtcggtt tcgcctcgtt
     1921 ctcgcactcg tacgtcgagt agaggtacgg cgtgtgcgcc tcgaactccg ccccgcaggt
     1981 gtcgacccgc ttgaagaccg cccgcacccc cgcggcgtac cggatcttgc ggacgtcgtc
     2041 ctcgccgacc ccgagaagct tcgagagtcg acggtcggag aagccgttcc ccttcatccg
     2101 gcgaaggaag gacgcgaacg cggccggttg cgtcgccgcc tccttccgga aggcctccgc
     2161 ccttgcgcgg atctcgccct ccatcgcgat gatctgccgg atgttttcga ggaaccaggg
     2221 gtcgattccg gtcgactccc ggatttcctc gaccgtccat ccgtcgcgaa acgcctcccc
     2281 gatgtaataa agacgaaggg agttcggctt gcgcagtttt tcagtgatcg tcttccggcg
     2341 tgccgccggg ttcggagggg ttgccggagg catcatctcc tcgaagccgt acacgctgtt
     2401 ctccagcgaa cggatcgctt tctgcaggga ctccttgaag gtgcggccga tcgccatcac
     2461 ctcccccacc gatttcatct gcgtgccgag cacatcctcg gtctggggca acttctcgaa
     2521 cgtgaaacgg gggatcttcg tcaccacgta gtcgatggtc ggttcgaagg aggcgggagt
     2581 ttcccgagtg atgtcgttgc ggatctcgtc gagggtgtac ccgacggcga gcttggccgc
     2641 gatcttcgcg atggggaacc cggtagcctt ggacgccagc gccgaggagc gggagacgcg
     2701 cgggttcatc tcgatgatca ccatctcgcc ggtctccggg tggacggcga actggatgtt
     2761 gctccccccg gtgtccaccc cgatctcccg gatgatgcgc aaggccgcgt tgcgcatgat
     2821 ctggtactcc ttgtcggtca gcgtctgcgc cggggcgaca gtgatcgaat cgccggtgtg
     2881 cacccccatc gggtcgacgt tttcgatcga gcagacgatg accacgttgt cggcaaggtc
     2941 gcgcatcacc tcgagttcga actctttcca gccgatgacc gactgctcga cgagggcggt
     3001 gtgcttcggg gaggcgtcca acgcccagcg gataccctcc tcgaactcct cacggttgta
     3061 ggcgatcccc gcgcccgttc ccccgagggt gaaggaaggc cggatgatcg ccgggtaccc
     3121 gatgtccggg atgaccccga gggcctcttc gagggagcgg atgtacccgg accgcgggac
     3181 gatcaacccc agcttctcca tcgccttccg aaacaggttc cggtcttccg ccttctggat
     3241 cgcttcaaag ctcgcgccga tcagttcgac gccgaacttc tgcaggatcc ccatctcatg
     3301 cagggaaacg gcgatgttca gccccgtctg cccgccgatg gtagggagca acgcgtcggg
     3361 gcgttcccgc tcgatgatct ttccgacgat ctccggggtg atcggttcga tgtacgtccg
     3421 gtcggcgaag tcggtgtcgg tcatgatcgt ggccgggttg ctgttgacga ggaccacctc
     3481 gtacccctcc tcacggaggg ccttgcaggc ctgggtcccc gagtaggcga actcgcacgc
     3541 ctgcccgatg atgatcggac cggagccgat gagcatgatc tttttcaggt cattgcgctt
     3601 gggcactatc gctcctgtat gaaaacgttc cggaatccga gccgctccgc ggcttcgatc
     3661 gcccggtcgt actcctttcg tgtcaccttc ctgtcgaacc cttcgacctc ggcggcccgc
     3721 cacgcaggaa aatattgccc catcagggag aggggaagat ccggaccgtg cctttccgac
     3781 agatgcgcca gcaccgcctc cgtctccccc acccttcccg gaaggacgag atggcgcacg
     3841 aggacccccc gcgcagcgat tccgtcgtcc ccgagggaga ggaacccgac ctggcgcatc
     3901 atctcgtcga tggcggcttc gttgtgccgc gcatagtcgg gggtgccgga agccgtgtcc
     3961 gcaagctccg cgttaagata tttcgcgtcc ggaaggtaga tgtcgacgac cccgtcgagg
     4021 agcgcgagcg tttcgagcgc atcatacccg ttcgtgttgt agaccaccgg gagatcgaac
     4081 cccattccgc gagccagaag aaccgcctcc agcatctgcg gcacgtgcga ggtgggggtg
     4141 acgaagttca cgttgtgaac ctccttttcc cgcaggcgaa gcatctcccc cgccagcgtt
     4201 tccgtgtcca tctcccgccc gttcccgtac tggctgatcg ggtagttctg gcagaagagg
     4261 cacttcatgt tgcaatggct gaaaaagatc gtccccgagc cgcgcgtccc cgaaatcggc
     4321 ggctcctcgc cgggatgcac ggaaacggcg gcgacgcgcg cgagcctgcc cgcgccgcag
     4381 aacccccgct ctccttcgag ccggttcacg ccgcactccc gcgggcacag gcgacaagcg
     4441 gagagcgcct ccatcgggat agaggcgctt cggtctccgc gggatacccg ctccgatgcg
     4501 cctccggatc gatggagctt cgtggtagcg ggcggctcgg ggcgtccgga gcacgagacc
     4561 tcccctgagg gggcgtgttt tccgagcgga ggcagggagg ccgggagcga ggaaaacccg
     4621 cagccgaagc gaaccaggga tggtgagcaa ggcgtccccg aaggggaggg tctcgtgcgg
     4681 aggaaagccc ccggccgttc gctcacagtt cctcctcccc gcaaaggtac cggtggaagc
     4741 gggtgaagag gtagcgggag tcgtgcgggc ctggggacgc ctcggggtgg tattgcacgg
     4801 agaagcaacg tttggaaggg acggagagcc cctccacggt tccgtcgttc aggttcacgt
     4861 gggtgatgcg gggggcgctg ccgagggagt cggcatcgac ggaataatta tggttctggc
     4921 tggtgatctc gacctttccc gtcgacaggt ccttcaccgg ctgattgcag ccatggtgcc
     4981 cgaacttcag cttgaacgtc tttcccccca gcgcgagccc catgatctgg tgcccgaggc
     5041 agatcccgaa catcgggacg tttccaagga gcgcgcggac cgttgcgacg gcatacggca
     5101 cgccttcggg gtcccccggc ccgttcgaca ggaagacgcc gtccggagag tgcgccagga
     5161 cctccgccgc ggtgacgccg gccggcacga cggtcacgtc gaacccgtgg gagaccatca
     5221 tccggaggat gttctgcttg atcccgaagt cgatcgcgac gatccgcggg acgcgcccga
     5281 acttgcccag gaacgcggcg gccggcaggt accccttctc gaggtcccag tcccccgtgg
     5341 tccaatggac cgcatggtcg gaagtgacct ccttcaccag gtcacgcccc acgagggaag
     5401 ggagttcccg cgctttgctc gcgaggcgat ccgcgtcgag atcggtcgcc gacaggaccg
     5461 ccatctggga ccccccgtcc cgaagacgcc gcgtgagggc gcgggtgtcg attcccgcga
     5521 tcccgcagac gtgataccgc cgaaggtagg cgtcgaggga ctccacgtgc cgccagttcg
     5581 acggcgggcc ccaggcctcg cggacgatga acccctcgac ccaggggcgg ttcgattcca
     5641 cgtcctccgg gttgattccc gtgttgccga tctccggata cgtcatcgtg acgatctgtc
     5701 ccttgtagga aggatccgtg agcacttcct ggtacccggt catgccggtg ttgaagacga
     5761 cctcgccgga gcattccccc tcgtaaccga aggaggtccc ctcgtaggcc gtgccgtccg
     5821 cgaggacgag aaaggccttc cggtcaggca tccgccgtga ctcccgcgat cgtcgcgtgg
     5881 cgaacccgcc cgccgcagat cgtcgcgacc gagcggccgc gcatcttcca cccgaagaag
     5941 ggactgttcc tcgacttcga gagcacgtct ccgggcgcaa attcccattc cgcatcgggg
     6001 tcgatgaccg tgacgtcggc gtcggcgccg gcggcgagcg ttcccttgcg gcgcagaccg
     6061 aggatccgcg cggggccggc ggagagcaga tcgacgatac gcgccggagc gacctttccc
     6121 ccggccagca gggaaagcga gagcggaaga gaggtctgca gcccgatgat gccgtttgcg
     6181 gcggcggtga actcgcactt cttcacgtac gactcgtgcg gggcgtggtc gcaggcgatc
     6241 gcgtcgaatg tgccgtcctg gatcccctcg aggatcgcca tccggtcctc ctcgccgcgc
     6301 agcggcgggt tcatcttcgc gttcgtgtcg tacccttcga gcgccgcgtc ggtcagtgtg
     6361 aagtattgag gagccgtctc gcccgtgacg tcgagaccgg cgcgatgggc catccggagg
     6421 agatccaccc ccatgcgggt gctgatatgc tggatgtgca gctttccccc cgtctgccgg
     6481 gcgatcagga tgtcccgggc gatggcgacc tcctccgccg cggaggggat ccccgggatc
     6541 ccaagcttcc gggcggtcca cccctcgtgc gcggccccgc cgccggccag cacgacgtcc
     6601 tcggcgtggg agaggatgcg atatccgaag ggacggacgt actccatcgc gcgccgaagc
     6661 aggagggagt tctccaccgg tttcccgtcg tccgaaaacg ccaccgctcc ggcgtcggcc
     6721 agttccgaga agtccgccat ctctttcccc tcgagcccga gggtcacggc ggccacggga
     6781 aagacgttgg cgcacccttc ccggcgcgct ttctcgatga tgtgccgggt gacctccggg
     6841 gaatcgttga ccggattcgt gttggccatg cagacgaccg aggtgaaccc gccggccgcc
     6901 gccgcacgtg tcccggtaag aatatcctct ttccactcgt atcccggatc acgcaggtga
     6961 acgtgcatgt cgatcaggcc gggcacgacc cacttcccct cggcggagat caccttcccc
     7021 tggaaccgcg cgggggcttc gcccgccagg aactcgcgga ctttcccgtc ctcc
//
LOCUS       DASVJL010000396         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1474728, whole genome shotgun sequence.
ACCESSION   DASVJL010000396 DASVJL010000000
VERSION     DASVJL010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474728"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VF357_08360"
     CDS             complement(<1..528)
                     /locus_tag="VF357_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524201.1"
                     /translation="MELIFEVSVNGRRGVRLPASDVPAATPLPNEFLRAEPAGLPELS
                     ELDVVRHFTQLSRRNIGVDTTFYPLGSCTMKYNPKALEEAARLFLPFHPMIALLPGGE
                     ALAQGTLAIIHRMGELLEEITGMDAVTCQPLAGAHGEMTGIMLIAAYHKAKGNRKKYV
                     IVPDSSHGTNPASAAM"
     gene            complement(519..1853)
                     /gene="gcvPA"
                     /locus_tag="VF357_08365"
     CDS             complement(519..1853)
                     /gene="gcvPA"
                     /locus_tag="VF357_08365"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX7524202.1"
                     /translation="MGFTPHTPEEIREMLATIGVGSIDDLFRPIPKELRVRSFDLPPG
                     MSEFEMMARLQEMAAQNGRVVPFVGGGYYDHVIPAAVDHLAGRAEFYTAYTPYQPECS
                     QGSLQALFEYQSAICRLTGLEASNASLYDGGTALAEAAMMALRVTGRGRIILDGAVNP
                     FHRAIVRTYLANLPVEIVELPPVNGMLDRQSLAGKLDDRCAAVMVQNPTFFGCVADYS
                     DLAAQVHKSGALLVSAVYAVSLGLLKPPGDMGVDIAVGEGQNLGNHLSFGGPAFGFIA
                     AKKQYIRNLPGRIVGETVDREGRRGFVLTLQAREQHIKRHKATSNICSNQSLCALRGL
                     IFLASLGKQGLVELARLNRDKAEYAKRALAAVRGVTIPSGGPTFNEFTIRLPKRADDV
                     VASLLQRGIAAGVPLGSYYRGEENSVVVTVTEKRTKGEIALLARELEGALWN"
     gene            complement(1855..2229)
                     /gene="gcvH"
                     /locus_tag="VF357_08370"
     CDS             complement(1855..2229)
                     /gene="gcvH"
                     /locus_tag="VF357_08370"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX7524203.1"
                     /translation="MSKTYYTKEHEWVKIDGNIATVGISDYAAHQLGDITFVEHPPVG
                     KAVKQFEVLCAIESVKAASDIYAPVSGTVLSVNEALNTRPEIVNESPEEAAWMATMKM
                     TDPSEVAKLMGRDRYDEYRKGL"
     gene            complement(2266..3360)
                     /gene="gcvT"
                     /locus_tag="VF357_08375"
     CDS             complement(2266..3360)
                     /gene="gcvT"
                     /locus_tag="VF357_08375"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX7524204.1"
                     /translation="MELLRETPLIGRHREQKALLAPFGGWNMPIQYEGGILAEHRWCR
                     EKATLFDVCHMGEFLYRGDMVDSGLEDVFTFSVASIPEGRSRYGFLLNEHGGVIDDLI
                     VFRLAKEEAMIVVNAATAGNDFGVIRSRLKAGAEFSDISIATGKLDPQGPLSRDVVTD
                     LLGRNISSIPYFRFITTDILGSKAIVSRTGYTGELGYEIFLPAEKTVELWDKLLKDPR
                     MKPAGLGARDLLRLEVGYSLYGNDLDETITPLEAGLEGFVNFDSEFIGKDALLRQRAT
                     GLKRSKVSFRVGSRRSPRHHYEICSGDRNIGTTTSGGFAPMLGCGIGLGLVSPDAAAI
                     GTKLTIRHENVSMPATVCELPFYRGGSVRK"
BASE COUNT          629 a         1090 c         1099 g          689 t
ORIGIN      
        1 catggcggcg gaagcggggt tggtgccgtg agaagagtcg gggacgatga cgtacttctt
       61 cctgttcccc tttgccttat gataggcggc gatcagcatg atgccggtca tctcgccgtg
      121 cgccccggcc agcggttgac acgtgaccgc atccatcccg gtgatctctt cgagcagctc
      181 tcccatgcga tggatgatcg ccagcgtgcc ttgggcgaga gcttcgcccc ccggcaacag
      241 tgcgatcatc ggatggaacg gcaggaaaag cctcgcggcc tcctccagcg ccttggggtt
      301 gtacttcatc gtgcaggaac ccagcgggta gaacgtggta tccacgccga tgttgcggcg
      361 ggatagctgg gtgaagtggc gcaccacgtc cagctcggaa agctcgggaa gcccggcggg
      421 ttcagcgcgc aagaactcgt ttggaagcgg ggttgccgca ggtacgtcgc ttgccggcag
      481 ccggacgccg cggcgtccgt tgacggaaac ctcgaaaatc aattccatag cgccccctcc
      541 agttcccgcg ccagcagggc tatctccccc ttggttcttt tctcggtcac ggtcaccacc
      601 acggagttct cctcaccccg gtagtacgaa ccaagcggca cgcctgcggc aataccccgt
      661 tgcaacagcg aagcaaccac gtcgtcggcc cgcttcggga gacggatggt gaattcgttg
      721 aaggtcgggc ccccggaggg gatggttacc ccccggactg cggccagcgc acgtttggcg
      781 tactcagcct tgtcacggtt gagccgcgcc agctccacaa gcccctgttt ccccaggctc
      841 gccaggaaga tcaggccgcg cagggcgcag aggctttgat tgctgcagat attggaggtc
      901 gccttgtggc gcttgatgtg ctgttcgcgg gcctggagcg tcaggacgaa gccgcgacgt
      961 ccttcgcggt cgacggtctc gcccacgatc cgaccgggca agttccggat gtactgcttc
     1021 tttgcggcga taaagccgaa ggccggtccg ccgaaggaga ggtggttccc gaggttctgg
     1081 ccctcgccca cggcgatgtc aacccccatg tctcccggcg gtttgagaag ccccagcgat
     1141 accgcgtaga cggccgatac cagcagcgcg ccactcttgt gcacctgggc cgcaaggtcg
     1201 ctgtagtcgg ccacgcagcc gaaaaaggtc gggttctgca ccatcaccgc ggcgcagcgg
     1261 tcgtcaagct tcccggccag gctctgtcgg tccagcatcc cgttcaccgg cggaagttcc
     1321 acgatctcca ccggaaggtt ggcgaggtag gtccgtacga tcgcccgatg gaaggggttg
     1381 acggcgccgt ccaagataat ccgacctcgg ccggtgaccc gcagcgccat catggcagcc
     1441 tcggccaagg ccgtgccgcc gtcgtagagg gaggcgttgg aggcttccag gccggtcaga
     1501 cggcagatgg cggactggta ctcaaagagc gcctgcaagg acccctggga gcattcgggc
     1561 tggtaggggg tataggcggt gtagaattcg gcgcggccgg ccaggtggtc caccgctgca
     1621 gggattacgt ggtcgtagta gccgccgccg acaaacggaa cgacgcggcc gttctgcgcc
     1681 gccatctctt gcagtcgggc catcatctcg aactccgaca tcccgggagg gaggtcgaag
     1741 gatcgcaccc gcaactcttt cgggataggg cggaacaggt cgtcgatgct ccctacgccg
     1801 atggtggcaa gcatctcccg gatctcttca ggggtatgtg gcgtgaaccc catctcagag
     1861 tcccttccga tactcgtcgt atcggtccct gcccatcagc ttcgccacct cggaaggatc
     1921 ggtcatcttc atggtggcca tccaggccgc ttcctcggga gactcgttga cgatctcggg
     1981 ccgggtattc agcgcctcgt tgaccgagag caccgtgccg gagaccggtg cgtagatgtc
     2041 gctggccgcc ttcaccgact cgatggcgca gagcacctcg aactgcttca ccgccttccc
     2101 cactggagga tgctccacga aggtaatgtc ccccagttgg tgggccgcat agtcgctgat
     2161 ccccaccgtg gcgatgttcc catcgatctt cacccattcg tgttccttgg tgtagtacgt
     2221 tttggacata ccgcttctcc ttggcgatga aggatcgaat tgtcgctact tgcggaccga
     2281 accacccctg taaaatggca attcgcagac cgtcgccggc atgctgacgt tttcgtggcg
     2341 gatggtgagc ttcgtcccaa tcgccgcggc gtccggagaa accaggccga gtccgattcc
     2401 gcagccgagc attggagcga agccgccgct ggttgtggtg ccgatgttcc tgtcgccgct
     2461 acagatctcg tagtgatggc gaggagagcg gcgggaacct acccggaagg acaccttgga
     2521 tcgtttgagt cctgttgccc gttgcctcaa gagggcgtcc ttgccgatga attccgaatc
     2581 gaagttgacg aacccctcca ggccggcttc cagcggggtg atcgtctcgt cgaggtcgtt
     2641 tccgtagagg ctgtacccca cctcgaggcg gaggaggtca cgcgctccaa ggcccgccgg
     2701 tttcatgcga gggtctttca gcagtttgtc ccacagttcg accgtcttct ccgcagggag
     2761 gaagatctca tagccgagtt cgcccgtgta gccggtgcgg ctgacaatcg ccttgctgcc
     2821 gaggatgtcc gtggtaatga agcggaaata cggtatggag gagatattcc ggccgagcag
     2881 atcggtcacg acatccctgg acaacggccc ctgggggtcc agcttgccgg ttgcgatgga
     2941 gatgtcgctg aattccgctc ctgccttgag gcgcgagcgg atgacgccga agtcgttgcc
     3001 ggcggttgcg gcattgacca cgatcatcgc ctcctccttg gccaggcgga agacgatcag
     3061 gtcgtcgatg acccctccat gttcgttgag gaggaagccg tagcgggacc gtccctccgg
     3121 gatggaggcg acggagaagg tgaagacgtc ttcaagaccg ctgtcgacca tgtcgccacg
     3181 gtagaggaac tctcccatgt ggcatacgtc gaagagggtg gccttctccc ggcaccagcg
     3241 atgttcggcg aggatccccc cctcatactg gatcggcatg ttccagccgc cgaaaggggc
     3301 caggagggcc ttctgttccc ggtgtctgcc aattaggggt gtttccctga gcaactccat
     3361 gaagtcacct ccttggcggg taccgggtgc ctcaatttac ccgatttaca ccccgatgta
     3421 aatccaaaca gtttccgaac ctcacgaacc tttcgcagca ccattcgatt accggaatcc
     3481 ggaaactcac gaaggtatcg tgcacct
//
LOCUS       DASVJL010000397         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_254117, whole genome shotgun sequence.
ACCESSION   DASVJL010000397 DASVJL010000000
VERSION     DASVJL010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254117"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VF357_08380"
     CDS             <1..274
                     /locus_tag="VF357_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524205.1"
                     /translation="GSAYYAPSAAAVQMCDSILLDKKMILPCAVLSSGKYDGCDGLFV
                     GLPAKLGAGGVEEVVKFKLAANEAEALKRSAAAVKELCEAVDRLGF"
     gene            333..1502
                     /gene="sucC"
                     /locus_tag="VF357_08385"
     CDS             333..1502
                     /gene="sucC"
                     /locus_tag="VF357_08385"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX7524206.1"
                     /translation="MNIHEYQAKAVLSKYGVTVPKGKVADTPAQAEVIAEEFGTPVVI
                     KAQIHAGGRGKGGGVKLAKDSAEARKYAHEIIGMTLVTPQTGPQGRKVKRVLVEQAGK
                     IKKELYLGIIVDRAVSQVVVMASTEGGMDIETVAARTPEKILKEWVDPAVGLVPFQAR
                     KLAFGLGIPKELTGKAVKLMTGLYNAFVATDCSLVEINPVVLTEDGDIVALDAKMNFE
                     SNGLFRHQDIQALRDFDEEDPTETEASNYDLSYIRLDGNIGCMVNGAGLAMATMDMIK
                     SCGGNPANFLDVGGGAGVEQVTNAFRLILSDKKVKAILVNIFGGIMRCDIIAEGVVTA
                     AKTLGLSVPLVVRLEGTNVEKGKEILAASKLNIIPAADMGDAARKVVLAAAGRAN"
     gene            1516..>1820
                     /locus_tag="VF357_08390"
     CDS             1516..>1820
                     /locus_tag="VF357_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX7524207.1"
                     /translation="MSIFIDKNTKVLVQGITGSVGTFHTKQMLEYGTKIVGGVTPGKA
                     GSKVEGVPVFNTVTEAVKATGANASVIYVPPAFAADAICEAFDAGLDLVVCITEGIP"
BASE COUNT          357 a          540 c          648 g          275 t
ORIGIN      
        1 ggggtcggcg tactacgccc cctcggcggc ggccgtccag atgtgcgact cgatcctgct
       61 cgacaagaag atgatccttc cctgcgcggt cctctcgagc ggaaagtacg acgggtgcga
      121 cgggctcttc gtcggcctgc ctgccaagct cggcgccggc ggggtggagg aggtcgtcaa
      181 gttcaagctg gccgcgaacg aggccgaggc gttgaagagg tcggcggcgg cggtcaagga
      241 gctttgcgag gcggtcgacc ggctcggatt ctgaagcacg cgcccgcgac ggatcggatc
      301 gatcacaaat caaccgggag gagccggagg atatgaacat acacgagtac caagccaagg
      361 ccgttctgtc caagtacggc gtcaccgtac cgaaagggaa ggtggcggac acccccgcgc
      421 aggccgaggt catcgcggag gaattcggca cgcctgttgt tatcaaagcc cagatccacg
      481 cgggaggccg ggggaagggc ggcggcgtga agcttgccaa ggattccgcc gaggcgcgga
      541 agtacgccca tgagatcatc gggatgaccc tggtgacccc ccagacgggc ccccagggga
      601 gaaaggtcaa gcgcgtcctg gtcgagcagg cggggaagat caagaaggag ctctacctcg
      661 gcatcatcgt cgaccgggcg gtctcgcagg tcgtcgtgat ggcctcgacg gaaggcggga
      721 tggacatcga gaccgttgcg gcccgcaccc cggaaaagat cctgaaggag tgggtggacc
      781 cggcggtagg tctggttccg ttccaggcgc ggaaactggc cttcgggctg gggatcccga
      841 aggaacttac cgggaaagcg gtgaaattga tgacggggct ttacaatgcc ttcgtggcta
      901 cggactgctc cctggtcgag atcaacccgg tcgtcctgac cgaggacggc gacatcgtcg
      961 cgctggacgc gaagatgaac ttcgagagca acggcctgtt ccgtcaccag gacatccagg
     1021 cgctgcggga cttcgacgag gaggacccga cggaaacgga ggcgtcgaac tacgacctct
     1081 cctacatccg cctcgacggc aacatcggct gcatggtgaa cggcgcgggg ctggcgatgg
     1141 ccacgatgga catgatcaag agttgcggcg gcaacccggc gaacttcctc gacgtggggg
     1201 gcggcgcggg cgtcgaacag gtgaccaatg cctttcgcct catcctgtcc gacaagaagg
     1261 tgaaggcgat cctcgtgaac atcttcggcg ggatcatgcg gtgcgacatc atcgccgaag
     1321 gcgtggtgac ggcggcgaag accctgggtc tctcggtccc cctggtcgtc cggctcgagg
     1381 ggacgaacgt ggagaagggg aaggagatcc tcgcggcgtc gaagctcaac atcattcccg
     1441 cggccgacat gggcgacgcc gcgaggaaag tcgtcctggc cgccgccggc cgggcgaact
     1501 aaggggggcg cgtccatgag catattcatc gacaagaaca ccaaggttct cgtccagggg
     1561 atcaccggct ccgtggggac gttccacacg aagcagatgc tggagtacgg cacgaagatc
     1621 gtcgggggcg tgacccccgg caaggcgggc tccaaggtgg agggcgtccc ggtcttcaac
     1681 accgtgaccg aggcggtcaa ggccaccggc gccaacgcgt cggtgatcta cgtccccccg
     1741 gcgttcgcgg cggacgcgat ctgcgaggcg ttcgacgcgg ggctcgacct cgtggtgtgc
     1801 atcaccgagg ggatccccat
//
LOCUS       DASVJL010000398         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_721959, whole genome shotgun sequence.
ACCESSION   DASVJL010000398 DASVJL010000000
VERSION     DASVJL010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721959"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VF357_08395"
     CDS             complement(<1..310)
                     /locus_tag="VF357_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7524208.1"
                     /translation="MNRFRKLSRRLASPRSQRGCSGGRRGFTLIEITIVLLIIGILAA
                     AATYTYRMMVSKARMTQAKTVLGHLTKTEATYFSDHTLYTDNIVLLGFDPVKYDYYQV
                     S"
     gene            complement(307..786)
                     /locus_tag="VF357_08400"
     CDS             complement(307..786)
                     /locus_tag="VF357_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524209.1"
                     /translation="MGADEDRRRTRIVDRRFQLGLAVRLILVLTILFAAGIALAFLPS
                     IYVLATTNDLKSLEPAAAEFLVLHKRLLPAALLSLAGIFFYVIFFSHRVAGPVYRINE
                     ILRALIEDRDPSVVKFRDGDYFQPTAALLKELSEKMRASRRIPPPRPGGNPSPGDAT"
     gene            857..>1661
                     /locus_tag="VF357_08405"
     CDS             857..>1661
                     /locus_tag="VF357_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX7524210.1"
                     /translation="MGTLRRSLSIRAGVVLQLALVATASLALIAVFALKVIEVTLQRR
                     HVEAGISVAGVVRRAVEKEIAENPGSSNISARMFSGSLSPYIYEVVLLPEPPPEGRPK
                     VLPVGEKAFPFLPVYPTVDVILPFGSLSPPIPSAAASVPRGIRVRFHSPGIAAEVGTF
                     VNVTLLLAAIDIAVIVIFGGVFLDRTVISPVRKLASAAEKVAAGDYSLRVEGVEGNEL
                     GQLADSFNRMVEGILEAQERLRRSEKETFRSEKLATVGRLAAGVAHEVGN"
BASE COUNT          269 a          537 c          550 g          305 t
ORIGIN      
        1 ccgagacctg gtagtagtcg tacttcaccg ggtcgaaacc gagcagaacg atgttgtccg
       61 tgtacagggt atggtcgctg aagtaggtgg cctccgtctt ggtgaggtgg ccaagcaccg
      121 tctttgcctg cgtcatccgc gccttgctga ccatcatgcg gtaggtgtac gtcgccgcgg
      181 ccgcaaggat gccgatgatc aggaggacga tcgtgatctc gatcagcgtg aacccgcgcc
      241 tgcctccact acaccccctc tgtgaacgtg gggaggcgag acgccgggac aacttccgga
      301 accggttcat gtcgcgtcgc cgggcgaggg attgcccccg gggcgaggtg gcgggattct
      361 ccgcgaggcg cgcatcttct ccgacagctc cttcagcagc gcggcggtgg gctggaagta
      421 gtcgccatcg cggaatttca cgaccgaggg gtcccggtcc tcgatgagcg cccggagaat
      481 ctcgttgatc cggtatacgg ggcccgcgac ccggtgactg aagaagatga cgtagaagaa
      541 aatccccgcg agggagagga gcgccgcggg caagaggcgc ttgtgaagca cgaggaactc
      601 ggcagcggcg ggctcgaggg atttcaggtc gttcgtcgtc gcgaggacgt agatcgacgg
      661 gaggaacgca agggcgatcc ccgccgcgaa gaggatcgtg agcaccagga tgagacgcac
      721 ggcgagcccc agttggaatc gccggtccac gatcctcgtg cgacgacgat cttcgtccgc
      781 gcccaaattc gcctccatct cccgtttttg ctattctaat ccgcgtgaac gcagagtcaa
      841 cgcggaatcc tttcccatgg gcacgctgag gcgctccctc tcgatccgcg ccggcgtcgt
      901 gctgcagctc gcgctggtcg ccaccgcttc ccttgcgctc atcgccgtct tcgcgctgaa
      961 ggtgatcgag gtcaccctgc agcgtcgcca cgtggaggcc ggcatctccg tcgccggggt
     1021 cgtccggagg gcggtggaaa aggagatcgc ggagaacccg gggtcctcga acatttccgc
     1081 caggatgttt tctgggtccc tctcccccta tatatacgag gtcgtcctgc ttccggaacc
     1141 gccgcccgag ggccgcccaa aggtcctccc cgtgggggag aaggcgttcc cgttcctccc
     1201 ggtgtatccc accgtggacg tgatcctccc gttcggttcg ctctctcccc ccatcccatc
     1261 cgctgcagct tccgtccccc gcggcatccg ggtccgcttc cattcccccg ggattgccgc
     1321 cgaggtcggg acgtttgtga acgtcacgct gctgctggcc gcgatcgaca tcgccgtcat
     1381 cgtcatcttc ggtggggtct ttctcgaccg gaccgtgatc tcccccgtcc gcaagcttgc
     1441 gtccgccgcc gagaaggtcg cggcggggga ttactctctg cgcgtcgaag gcgtcgaggg
     1501 gaacgagttg gggcagctcg ccgactcctt caaccggatg gtcgagggga tcctcgaggc
     1561 gcaggaacgg ctgcgccgct ccgagaaaga gacgttccgg tccgagaagc tcgcgaccgt
     1621 ggggcggctc gcggccgggg tcgcccacga ggtggggaac c
//
LOCUS       DASVJL010000399         5342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1286019, whole genome shotgun sequence.
ACCESSION   DASVJL010000399 DASVJL010000000
VERSION     DASVJL010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286019"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VF357_08410"
     CDS             complement(<1..336)
                     /locus_tag="VF357_08410"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX7524211.1"
                     /translation="MTASAGREVREFVKEIYRSRTIPSMLGKILSLVKDENSSPQELF
                     LLISHDPALAERVIRVANSVMFGHSGEVRDLRHAVMFLGYERLRSIAMGMGVMDVFSI
                     SARNSLDIQN"
     gene            complement(384..1979)
                     /locus_tag="VF357_08415"
     CDS             complement(384..1979)
                     /locus_tag="VF357_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7524212.1"
                     /translation="MKITLSKKLYGLVGLMSLMMLIGTGAAVYSLRSMLGDYRTLVTS
                     DVAQYSAAMEARVALGEAVQGTKNFMTRMDQDNVKEFKNSIEKIKAASQTLGKLADTD
                     ADRQALQKVEKERVLYEQKGNELIAAILSGKDAVSVDAVFKGIDDPLRVALEELRNLC
                     LKVQKENFARDASTAARTQWLFVVTALVGIALALGFATLTIRKILKAVTAVSDVTSMA
                     ATGDMTKDVTVLSDDEVGQMGLGFNRMMGDLRRIAGQIKGMTNTLASSTEEVSATTES
                     LMQGAKEQSRQTEQAAAAVTEVSQTVMDVARNASEASKASLDMSRIAGEGRKKVEDTV
                     AGMHGIAGTVKEAANTVGELGNASKEIGTIINTINDIADQTNLLALNAAIEAARAGES
                     GRGFAVVADEVRKLAERTGKATKEIAAMIEKIQEETARSVTGMQTGLAEVEKGVALAE
                     EAKSALERIVSASGVSTDMIQRIAAAAEEQSAATEQVSSNMDAIARVTLTTESAIDQI
                     RVTVLDLSRMAGELNSSAAWFKV"
     gene            complement(2001..2477)
                     /locus_tag="VF357_08420"
     CDS             complement(2001..2477)
                     /locus_tag="VF357_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7524213.1"
                     /translation="MKALAADNKIIQLVTFRLGGEEYGVDILKVHEINRMMDITAVPN
                     APNCIEGVINLRGKVIPVINLRMKFGLPHRQADARTKIVVVDVGMPAGIIVDSVSEVL
                     RIASDVIEPAPPMTASIGSEYILGVGKLKDRLLILLDIEMLLGVEGKIMADAVAAI"
     gene            complement(2553..3599)
                     /locus_tag="VF357_08425"
     CDS             complement(2553..3599)
                     /locus_tag="VF357_08425"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX7524214.1"
                     /translation="MIRVLIVDDSAFMRNALTKMLSSDPEITVVGTAWNGLDAIEKVA
                     ELKPDLVTMDIEMPRMDGIEALRRIMATNPVPVIMVSSLTTEGARATLDALDLGAVDF
                     VPKNLSDLSVNIVKVREILIEKVKQIGRQVPGRRRPSRPAPASAAPAPLRSVYRTERR
                     IGIVAIATSTGGPKALQCVIPMLPKDLSVPVIIVQHMPPAFTGPFAQRLNEISGLTVK
                     EAEEGEPLRPGIVLVAPGRGHLSVRKGRPGENVVAVSENRPDLIYRPSADVMMSSIAE
                     MYPGRALGVILTGMGNDGEKGMRSIKSSGGRTIAQDEETCVVYGMPRAVVEAGLADKV
                     VPLGEVAGEIVNAV"
     gene            complement(3646..4839)
                     /locus_tag="VF357_08430"
     CDS             complement(3646..4839)
                     /locus_tag="VF357_08430"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEX7524215.1"
                     /translation="MGQYIGFNLGAEEYSIPILKVLEIINVPGMTRLPKTPEYIEGVA
                     NLRGRVVPIVNIKKIVKLDGASGGGDKVIVLSNGKVTFGILVDGITGVISIEDSSIEP
                     PDRLLNGSCDRIVGVAKYKKRLIVLLDMKNLLPADGLEMFDENIVSVNETIDIDKVDV
                     VKKVQTMGGEVEFHELRDAKEFLSRKAGGDDPNRRIVESLVAFMEAVSSKDYEKADAV
                     VARLADSSSVGLFAEVGKVTRRLHDSLKSFKDAIDPKLSSLASCEVPNAIDRLQFVID
                     KTEEAANKTMGIVEKHLLEMDELSARIRKLDGPEDAVKYIKDFKNRLEDDLTEIITTQ
                     SFQDITGQTIKKVILLVGEIEKELVGMVAAFGLKTEAGPLAKEAVREMVSQSDVDDLL
                     KDFGF"
     gene            complement(4871..>5342)
                     /locus_tag="VF357_08435"
     CDS             complement(4871..>5342)
                     /locus_tag="VF357_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7524216.1"
                     /translation="THKDEGTTFRISIPMTLAIIQSLMVRAGGGQYAIPLALVEEILR
                     VDSKELAETAGQKVLVIRDRIHPFFELSELLGKGSPPDTGYRYAVVVAVGENRFCLGV
                     DKFVGEEEVVIKPIDGLTMESSHMIGATITGEGKVVLILDLASISRKLSGAIRT"
BASE COUNT          870 a         1815 c         1548 g         1109 t
ORIGIN      
        1 gttctggata tccagcgagt tccgtgccga gatggagaaa acatccatga cgcccatgcc
       61 catggcgatc gagcggagcc gttcgtagcc gagaaacatc acggcgtgcc gcaggtcccg
      121 gacctcgccg gaatggccga acatgaccga gttcgcaacc cggatgaccc gctccgcgag
      181 cgccgggtcg tgggagatga gaaggaacag ctcctggggg gaggaattct cgtcctttac
      241 gagggacagg attttcccca gcatcgacgg gatcgtccgg gaccggtaga tctccttcac
      301 gaactcccgg acctctcgcc ccgcgcttgc cgtcatggtg atccctcacc gggggtcttt
      361 ccggtcggaa gaccccggcc ccgtcagacc ttgaaccagg cggcggagct gttgagctcg
      421 ccggccatcc tggagaggtc gagcaccgtc acccggattt gatcgatcgc cgactcggtc
      481 gtcagtgtga cgcgcgcgat cgcatccatg ttggaggata cctgctccgt tgcggcggac
      541 tgctcctcgg ccgcggcggc gatccgctgg atcatgtccg tgctgacacc ggaggcgctt
      601 acgatccgtt cgagggccga cttcgcctcc tcggcgaggg cgacgccttt ctcgacttcc
      661 gcgaggccgg tctgcatccc ggtgacggac cgcgccgtct cctcctggat cttctcgatc
      721 atggcggcga tctccttggt cgccttgccg gtccgctcgg cgagcttgcg aacttcgtcg
      781 gccacgaccg caaacccccg gccggactcg cccgcacgcg ccgcctcgat cgccgcgttc
      841 agggcgagca ggttcgtctg atcggcgatg tcgttgatcg tgttgatgat cgtgccgatc
      901 tccttgctcg cattgccgag ctcccccacc gtgttcgcgg cctccttgac cgtgccggcg
      961 atgccgtgca tcccggccac cgtgtcctcg accttcttgc ggccctcccc cgcgatccgg
     1021 ctcatgtcca gcgacgcctt ggacgcctcg gacgcattcc tggccacgtc catcaccgtc
     1081 tgcgagacct ccgtgaccgc cgccgccgcc tgctccgtct gccggctctg ctctttcgcc
     1141 ccctgcatga gggactccgt ggtggcggaa acctcctccg tgctgctcgc cagggtgttc
     1201 gtcatcccct tgatctggcc cgcgatccgg cgcaggtccc ccatcatcct gttgaacccc
     1261 aggcccatct ggccgacctc gtcgtcgctg aggaccgtga cgtccttggt catgtcgccg
     1321 gtcgcagcca tggaggtgac gtcgctcacg gccgtgaccg ccttgaggat cttccggatc
     1381 gtgagggtgg cgaaccccaa cgcgagggcg atcccgacga gggccgtgac gacgaacagc
     1441 cactgggtcc tggcggcggt ggacgcatcc cgtgcgaagt tctccttctg caccttcagg
     1501 cacagatttc tcaattcctc caaggcaacg cggagggggt cgtcaatgcc cttgaacacc
     1561 gcgtcgaccg atacggcgtc cttgccggac aggatcgcgg cgatcagttc gttccccttc
     1621 tgctcgtaga ggacgcgctc cttttccacc ttctggaggg cctggcggtc cgcatcggtg
     1681 tcggcgagtt tcccgagcgt ctgcgaggcc gccttgatct tctcgatcga gttcttgaat
     1741 tccttgacgt tgtcctggtc catccgggtc atgaaattct tggtgccctg cacggcctca
     1801 cccaacgcca cccgcgcctc catcgcggcg gagtattgcg cgacatcgga ggtaacgagt
     1861 gtacggtaat ccccgagcat cgagcggagg gaatacacgg ccgctcccgt cccgatcagc
     1921 atcatgagcg acatgagtcc caccagaccg tagagctttt tcgagagtgt gattttcatt
     1981 gcgcctttcc cctcaccttt ttatatggcg gccactgcat cggccatgat cttgccttcg
     2041 acacccagga gcatctcgat atccaggagg atgagcaacc ggtccttgag cttgcccacg
     2101 cccaggatgt actcggagcc gatgcttgcc gtcatcgggg gggccggctc gatgacgtcg
     2161 gaagcgatcc gcagcacctc ggagaccgag tcgacgatga tccccgccgg catgccgacg
     2221 tccacgacga cgatcttcgt gcgggcgtcg gcctgcctgt gcgggagccc gaacttcatc
     2281 ctcaagttga tgaccgggat gacctttccc cggaggttga tgacgccctc gatgcaattc
     2341 ggggcgttcg gcaccgcggt gatgtccatc atccggttga tctcatggac cttcaggatg
     2401 tccaccccgt actcttcgcc acccaaccgg aacgtcacca actggataat cttattgtcg
     2461 gcagccaacg ctttcataaa tctcctccag gaaattttcc cgtcgaacct tttgatttct
     2521 tatcggtaca atccttcaaa cctttaaccc ggtcagacgg cgttcacgat ttcgcccgcc
     2581 acttctccga gggggaccac cttgtcggcc agccccgctt cgaccaccgc ccgcggcatc
     2641 ccgtacacga cgcacgtctc ctcgtcctgg gcgatggtcc gcccccccga ggacttgatc
     2701 gaccgcatcc ccttctcgcc gtcgtttccc atgccggtca ggatcacccc gagcgcacgc
     2761 cccggataca tttcggcgat ggaggacatc attacgtccg cggacgggcg gtaaatgaga
     2821 tcgggacggt tctcggagac ggccacgacg ttctcccccg gtcgcccctt ccgtacgctc
     2881 aggtgaccgc gtccaggggc cacgaggacg atcccgggcc tcaacggttc cccttcctcg
     2941 gcttccttca ccgtcaaccc gctgatctcg ttcagtctct gcgcgaacgg ccccgtaaac
     3001 gctggcggca tgtgctgtac gatgatcacc ggcacggaga ggtctttcgg gagcatcggg
     3061 ataacgcact ggagcgcctt gggcccgccg gtcgacgtcg cgatcgcgac aatgccgatc
     3121 cggcgctcgg tccggtaaac ggaacgaaga ggtgcggggg ccgccgatgc gggagcggga
     3181 cgggacggcc ttcttctgcc cggaacctgc ctcccgatct gcttgacttt ttcgataagg
     3241 atctcgcgga ccttgacgat gttgaccgag aggtccgaca ggtttttcgg cacgaagtcg
     3301 acggccccga ggtcgagcgc atcgagggtc gcccgggcgc cttccgtcgt gagggagctg
     3361 accatgatga ccgggacggg gttcgtcgcc atgattcgac gaagcgcctc gatgccgtcc
     3421 atccggggca tctcgatgtc catcgtcacg aggtccggct tgagttccgc gaccttctcg
     3481 atcgcgtcga gaccgttcca cgcggtcccg acgaccgtga tctccggatc cgacgagagc
     3541 atcttcgtca gcgcgtttct catgaacgcg gaatcgtcca cgatgagaac ccggatcatc
     3601 ctcgcctgtt cccctctccc gccctcgcct gcaggccgcc gcgggtcaga atccgaaatc
     3661 cttcaacagg tcgtccacgt ccgactgcga caccatctcg cggaccgcct ccttcgccaa
     3721 cggccccgcc tcggttttca ggccgaaggc cgcgaccatt ccgacgagct ctttctctat
     3781 ttccccgacg aggaggatga ccttcttgat cgtctgcccc gtgatgtcct gaaaggactg
     3841 ggtcgtgatg atctcggtca ggtcgtcttc aagccggttc ttgaaatcct tgatgtattt
     3901 caccgcgtcc tcgggcccat cgagcttccg gatccgcgcc gacagttcgt ccatctcgag
     3961 gaggtgcttc tcgacgatcc ccatcgtctt gttggcggcc tcctccgtct tgtcgatgac
     4021 gaactgcagc cggtcgatgg cgttcggcac ttcgcaggag gcgagcgagg agagcttcgg
     4081 gtcgatcgca tccttgaaac tcttcagcga gtcgtgaagc cgcctcgtga ccttgccgac
     4141 ctccgcgaac aggcctaccg aagaggagtc cgcgagccgc gccacgacgg cgtccgcctt
     4201 ctcgtagtcc ttcgaggaca ccgcctccat gaacgcgacc agggattcga cgatccgccg
     4261 gttgggatcg tcccccccgg ccttcctgct cagaaactcc tttgcatcgc gcagttcatg
     4321 gaactcgacc tcccccccca tcgtctggac cttcttgacg acatcgactt tgtcgatatc
     4381 gatggtctcg tttacgctga cgatgttctc atcgaacatt tcgaggccgt ccgccgggag
     4441 aaggttcttc atgtcgagga ggacgatgag cctcttcttg tacttcgcca ccccgacgat
     4501 ccggtcgcac gatccgttca ggagccggtc cggaggctcg atcgaggagt cctcgatgga
     4561 gatgaccccg gtgatgccgt ccacgagaat tccgaaggtg accttcccgt tgctgagaac
     4621 gatcaccttg tctccgccgc cggacgcgcc gtcgagtttc acgatcttct tgatattgac
     4681 gatcgggacc acgcggccgc ggaggttcgc caccccttcg atgtactccg gggtcttcgg
     4741 cagacgggtc atcccgggca cgttgatgat ctcgagtacc ttcagaatcg ggatggagta
     4801 ctcctccgcg ccgagattga atccgatgta ttgccccatg tcccctccgt ctccggtcgc
     4861 ccggatcttc ttacgtccgg atcgccccgg acaactttct tgagatgctg gcaaggtcga
     4921 ggatgagaac gaccttgcct tcccccgtaa tggtggcgcc gatcatgtgg gacgactcca
     4981 tcgtgagtcc gtcgatcggc ttgatcacga cctcctcctc gccgacgaac ttgtcgaccc
     5041 cgagacagaa ccggttttct ccgacggcaa cgacgacggc gtaccgatac ccggtgtccg
     5101 gcggcgaccc cttcccgagc agctccgaga gttcgaagaa ggggtggatc ctgtcgcgga
     5161 tgacgagcac cttctgcccg gcggtctccg cgagttcctt cgagtcaacc cggaggatct
     5221 cctccacgag ggccagtggg atcgcgtact gacctccgcc cgcccgaacc atcagcgact
     5281 ggatgatggc gagggtcatc gggatgctga tccggaacgt cgtcccctcg tccttatgcg
     5341 tt
//
LOCUS       DASVJL010000400         7380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_912718, whole genome shotgun sequence.
ACCESSION   DASVJL010000400 DASVJL010000000
VERSION     DASVJL010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912718"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(594..1163)
                     /locus_tag="VF357_08440"
     CDS             complement(594..1163)
                     /locus_tag="VF357_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524217.1"
                     /translation="MKEGAEDHTDQQLKPDQRTCLPERDPQTTDNGIEEKKHKRAQRT
                     FNRRLVWFTGGLLFASIVANLIPIYQGSIAKQSADAATKAAATAENTFNLNKDFASKT
                     LEEMQKQGKALQDASNAAKAQTHISQKTLDAAISISRTDQRAWVGVIRVSSNEPKIGQ
                     PLPVFIAYKNTGKTPAKNFTVSNAVEPVE"
     gene            complement(1293..1991)
                     /locus_tag="VF357_08445"
     CDS             complement(1293..1991)
                     /locus_tag="VF357_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7524218.1"
                     /translation="MDRNVVEQYRLAMFGRMVMGVAHEIDNHLSVVLGFSELIQLAAA
                     NEQKVRDGAGKILSAGGKIEAIVQQFSRYVRPHAPSPEPLVPGEMIREILLFSRYDLG
                     RDNVIVLPIPEVPPGSLYADRRDFGLALLALLFNAAEAMSEKGGELSVRVAIDAAGWE
                     FTVSDQGPGIPAGFDEKIFEVGFTTRTGPVHVGLGLPVARHLAGQAGGTVRIASRAGG
                     GCEATIRVPLEGGA"
     gene            complement(2007..3401)
                     /locus_tag="VF357_08450"
     CDS             complement(2007..3401)
                     /locus_tag="VF357_08450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX7524219.1"
                     /translation="MWWVRFALAWCGLLALTAGASHFLGGFSTGPGVFLAGGAAGEFV
                     LAVASSILLDRTVFRHVQRLQNALTEGADGPLPPVKGPLAPLAEAISARMERSVATLR
                     EAVRDREESLAQVERLRRAFEDTRTNLESNVQYLRTMAEVSTVIAGTLDPEELYRIIP
                     ERVMAKLDLNDFCIMLYSWETKRLVAKSVSLSDGGTMPEFTLAPGEGVVGKVFTTGEP
                     AWIPDVRSSTDFLHYGGRRTDVRSFMCVPLVSKGKNIGVLMLNHPEPNAFEPELLPTM
                     RVLASYLAIAIENAGMFGLVKSLAEKDSLTLLYNHGSFHEKLAIELERANRYVRPMAV
                     IMLDIDCFKEINDRYGHTTGDRVLALVAGALGAHLRKTDIAARYGGDEFAVILPETDL
                     SSAAVIAERIAEGISNVRLDIGGGSVVSFTASIGYASCGHDAPGRGEILNIADRLMYD
                     SKRRGPGGVLGIQI"
     gene            complement(3502..6270)
                     /gene="bamA"
                     /locus_tag="VF357_08455"
     CDS             complement(3502..6270)
                     /gene="bamA"
                     /locus_tag="VF357_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX7524220.1"
                     /translation="MERSKFLPFLLAASLLFFPRGSAVAADPPAPAVSGPIISSVSFQ
                     VSSPFLISYEELTGLVKVRPGDRLTREGVRASIRGLYEKSVFREVTAFARETDGKVDL
                     LFFLRPFPLVAEIEVAGTERLTPAQIISASRLKRGTPVEEKDLSGAEEAVRAFLQRKG
                     FVRGTASVSVTCNVENGGGKVLVTVAEREPGTVGNLRFPGATRFTPEEMARFLGAEAG
                     KPYDFRRWEAGLSRVRSEYKREGFLTVHLSDTVDRCEPSSDLLCPAVQVEEGPRYDVR
                     WEGIAAFTPDRLSEVGGLRGDEEISEGALVRDLRERLVAFYRERGYLLFDATVTVEEP
                     SAGRTPLIVSVVEGKRGYIKDIRFSGNRGLSEKTLRGQMTTRGRGVFHWITTSGQYRD
                     EDWNDDMNAVVGLYQKSGYARMKVLGVDNAWDDRGGIVKTIRVEEGTRYRVREIVFLG
                     NDHFLRSEFLALMRNKEGAYLDYPGAEADQEAVTAHYRESGYLDVRVESEVIFDADAS
                     SVLRFAIVEGPRYRLGNIVVRGTLLTRAAAILRENPIAPGGTAGEKDLLRFQKAVYAT
                     GLYKSVRVQRVKRPEEGILDLVFEVEEALFFEVEFGGGWGTDTGLRGLLGAKEKNLDG
                     LGRSVSVQTIVSQKEEKLIGDFREPWIFGNRWKWEGGLTGLYDKAERISFNLRKASVV
                     ASITRKVLERSSVSLQYELSRDEVSNVAPGAVLSPEDQGYATIAAVRALAVLDFRDDP
                     FNPRKGTLLSGSAELAALPFGSQVDYYKLSGQGSFYFSVYRHSTIVLSGRAGMARAFG
                     RTQEVPIQKRFFLGGRSTVRGFKEDALGAKAADGTPTGGDMMVNLNTELRVPLRYGFI
                     GAVFVDAGSVWFANDAVNGFDLRKSSGLGLRYLTPVGPIGLDYGWKLDRREGESAAEW
                     HFTIGAVF"
     gene            complement(6257..>7380)
                     /locus_tag="VF357_08460"
     CDS             complement(6257..>7380)
                     /locus_tag="VF357_08460"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX7524221.1"
                     /translation="AVISREKIVFEHFEARSGGGNIDGWGEVPLKMDAGQRLYFSVDF
                     LDVRYPYPDDFRPVVQGHVELIGPVEDLLVTGDVEVQSARYTRVLYPEKALVDFSRRL
                     SDVVARREKSEFRVRLDINVVADRTIRIKNNLADLKAGGEFQVEGDTRKVIILGTFDV
                     YEGYVELYGSRYDLKRATVDFQDPRRINPRLDARGETKKGSYNIAVLVSGTFEKPEVD
                     FTSDPPLSRTDIVSLLAFGVTTQNTASSGTGSSSGGGSTAAIAIGSTVGGVNEKIRST
                     VGLDKFAIEPGYSSTTKTFEPKFVVGKSFGDRASVSMATSVGTSADSTATAEFKLREH
                     VFLQGSWQSATTTTEGDLGADLKFRYRYQQWKDFFRGKE"
BASE COUNT         1367 a         2429 c         2132 g         1452 t
ORIGIN      
        1 caagcggaac atcttcatgg atcacccccg gttaatcttt caaaccactt gtttctccgt
       61 ggtcgagacc attccggtgc ctgttgctga gaacatacag ggtatctcgg ggtgggacaa
      121 tgataacccg atggccctga atggaggtgg gcgtcctcct cgcccgagca gttcgcgcca
      181 cttcctattc gacagccgct ccatcatccc gttcatcatc gtcagcccaa cactccgctt
      241 ctgcagcggg cgctcattga gaaaaagggg agccgttaaa ttcagcctgt cggtaggcag
      301 cagttatttg tctgaatcat tgtgttcatc acacataaga tatccgccaa accggggatt
      361 atatatataa tagaatattg tccaatgcga tttgttcaat atgtcgtcgt acgtaatttt
      421 cccataaata tacaaagtaa ttttaccaga attcagtaca tcgagtgatt cttgcgtaac
      481 ttttccatcg atagtttgga ttgggtaaac tttagatatt gtttctgcat taggaaggag
      541 tattccacga ctaattggag ccttggattt ggatatgtag gttggcttat gattcattcc
      601 accggttcga ccgcattgga tactgtgaaa ttttttgctg gagttttccc ggtattttta
      661 taagcaataa aaacaggtaa tggttggcca atttttggtt cattactcga aacacgaata
      721 acacctaccc acgcccgctg gtccgttctg gaaatggata tggcagcgtc caaggtcttt
      781 tgtgatatgt gcgtctgcgc ttttgcggca ttggacgcat cttgcaaggc cttaccttgc
      841 ttctgcattt cttccagcgt cttacttgcg aagtctttat tgagattgaa tgtgttctcg
      901 gctgtggccg cagcttttgt cgcagcatcg gcggactgtt tagcaatgga cccttggtat
      961 atcgggatga ggttggcaac gatgctggcg aaaagtagtc caccagtaaa ccagaccaat
     1021 cgcctattaa acgttcgttg tgctcgtttg tgcttttttt cttctattcc gttatctgtt
     1081 gtttgaggat cacgttcagg gagacacgtt cgttgatccg gtttcagttg ctgatccgta
     1141 tgatcctctg caccctcttt cacctacacc cgtttccgaa cccggcacga taatgttgct
     1201 ggggagcggg ttggttggac ttgttggata tggaaggaag tggatgaaga agtaggttgt
     1261 gcatcgtccg cgctcattag accggaaggc gatcaggccc cgccttccaa cggcacgcgg
     1321 atcgttgcct cgcagccgcc ccccgcccgg gacgcgatgc gcacggttcc cccagcctgc
     1381 cctgcgaggt gccgcgcgac cgggagtccc aagcctacgt gtaccggtcc tgtgcgggtg
     1441 gtgaacccga cctcgaagat cttctcgtcg aaccccgcgg gaatcccggg cccttggtcg
     1501 ctcaccgtga attcccaacc ggcggcgtcg atggccactc gcactgaaag ttccccgccc
     1561 ttctccgaca tggcctccgc tgcgttgaac aggagcgcga ggagtgccaa cccgaaatcc
     1621 cgcctgtcgg cgtaaaggct ccccggaggg acttccggaa tcggcagaac gatcacgttg
     1681 tcgcggccga ggtcgtaccg cgagaacagg aggatctcgc gaatcatctc cccggggacg
     1741 agcggttccg gggagggagc gtgcggacgc acataccggg agaactgctg gacgatcgcc
     1801 tcgatcttcc ccccggcgga caggatcttt ccggcgccgt ccctgacttt ttgctcattg
     1861 gccgcggcca gctggatcaa ctcggagaag ccgagcacga cgctcagatg attgtcgatc
     1921 tcgtgggcta cgcccatcac catgcgcccg aacattgcga gccgatactg ctcgacgaca
     1981 ttccgatcca ttgggcctcc aaattcctaa atctgtatcc cgagcacacc tccggggccc
     2041 cgccgcttcg aatcgtacat gaggcggtcg gcgatgttca ggatctctcc ccgcccgggt
     2101 gcgtcgtggc cgcaggatgc gtacccgatg ctcgcggtga aggagacgac gctccccccc
     2161 ccaatgtcca gccggacgtt cgagatcccc tcggcgatac gctccgcgat caccgccgcg
     2221 gaggaaaggt cggtctccgg aaggatcacc gcgaactcgt cccctccgta tctcgccgcg
     2281 atgtcggtct tccgcaggtg ggcccccagc gcgcccgcta ccagcgcgag cacgcgatcc
     2341 cccgtcgtgt ggccatatcg gtcgttgatc tccttgaagc agtcaatgtc cagcatgatg
     2401 accgccatcg gccggacgta ccggttcgca cgttccagct cgatggcgag cttctcgtgg
     2461 aacgatccgt ggttgtagag caacgtcagg gagtccttct cggccagcga tttgaccaac
     2521 ccgaacatcc cggcgttctc gatggcgatg gcgaggtagg acgcgagcac ccgcatcgtc
     2581 ggaagcagct ccggctcgaa cgcgttcggc tcggggtggt tgagcatcag caccccgatg
     2641 tttttcccct ttgacacgag ggggacgcac atgaacgagc ggacgtccgt gcgacgccct
     2701 ccgtaatgca gaaaatccgt ggaggatctg acgtcgggaa tccaggcggg ctcgccggtg
     2761 gtgaacactt tccccacgac cccttccccg ggggcgaggg tgaactccgg catcgtcccc
     2821 ccatcgctca acgagacgga tttcgccacc agccgcttcg tctcccagga gtaaagcatg
     2881 atgcagaagt cgttcaggtc cagcttcgcc atcacccgtt ccggaatgat ccggtacagt
     2941 tcctcgggat ccagggtccc ggcgatgacg gtggagacct ccgccatcgt ccgaaggtac
     3001 tggacgtttg attcgaggtt tgtccgcgta tcctcgaagg cgcgccggag gcgttcgacc
     3061 tgtgcgaggc tctcctcgcg atcccggacc gcctcgcgaa gcgtggcgac cgaccgctcc
     3121 atccgggcgg agatcgcctc cgccaacggg gccagaggcc ctttcaccgg cggcaggggg
     3181 ccgtcggcgc cttccgtcag ggcgttctgc agccgttgga catgccggaa cacggtccgg
     3241 tcgaggagaa tggaactggc cacggccaga acgaattcac cggcggcgcc ccccgccagg
     3301 aaaacgccgg ggcccgtgga gaaacctccg aggaaatggg acgcccccgc cgtcaacgca
     3361 agaagaccgc accaggcgag cgcgaaccgc acccaccaca cggaacgatc cggaaagacc
     3421 cctccgaccc tgtcgtttcc ctgcggcatg aggcgaaatt accacgaatc ccccttatct
     3481 gaaacggagg attgcggttc gtcagaacac cgccccgatg gtgaagtgcc actccgccgc
     3541 ggactccccc tcgcgacggt ccagtttcca gccgtagtcg aggccgatcg gcccgacggg
     3601 cgtcaggtag cgcaggccca gaccggacga tttccgaagg tcgaagccgt tcaccgcgtc
     3661 gttggcaaac cagacgctcc ccgcgtcgac gaacaccgcc ccgatgaacc cgtaccggag
     3721 cggaacgcgc agttcggtgt tcaggttcac catcatgtcg ccgccggtgg gtgttccgtc
     3781 cgctgccttg gcccccagcg cgtcctcctt gaaaccgcga acggtgctcc gccctccgag
     3841 gaagaaccgc ttctggatcg gcacctcctg cgtgcgcccg aaggcgcggg ccatgccggc
     3901 cctgccggag agaacgatcg tgctgtgccg gtacacgctg aagtagaaac taccctggcc
     3961 cgacagcttg tagtagtcga cctgggagcc aaacggcagc gccgccagct ccgccgaccc
     4021 ggaaaggagg gtccccttcc tcgggttgaa cgggtcgtcg cggaaatcga gcaccgccag
     4081 cgcgcggacc gcggcgatgg tggcgtatcc ctggtcttcg ggtgaaagga ccgcgccggg
     4141 cgccacgttc gacacctcgt cccgcgagag ttcgtactgc agcgacacgg aagatcgttc
     4201 gagcactttc cgggtgatgc tcgccacgac gctcgccttc cggaggttga agcttatgcg
     4261 ctcggcctta tcgtacaatc ccgtgagccc gccttcccac ttccaccggt tgccgaagat
     4321 ccacggctcc cgaaaatcgc cgatcagctt ctcctccttc tgactcacga tggtctgcac
     4381 ggacacgctc cgcccgagcc catcgaggtt cttctccttc gccccgagga gtccccgcaa
     4441 gccggtgtcg gtgccccacc cgccgccgaa ctccacctcg aaaaagaggg cctcctcgac
     4501 ctcgaacacc aggtccagga tgccctcctc cggacgcttg acgcgctgga cgcgcacgct
     4561 cttgtagagt cccgtggcgt agaccgcctt ctggaaccgg agcagatcct tctctccggc
     4621 cgtccctccc ggcgcgatcg ggttctcgcg aaggatcgcc gcggcccggg tgaggagcgt
     4681 cccccgtacc acgatgttcc cgaggcggta gcggggcccc tcgacgatcg cgaagcgcag
     4741 cacggaggag gcgtccgcgt cgaagatcac ctccgactca acgcgcacgt ccaggtagcc
     4801 ggactcccga taatgcgcgg tcaccgcctc ctggtccgct tccgccccgg ggtagtcgag
     4861 gtacgccccc tccttgttcc tcatgagcgc gaggaattcg gaccggagga agtggtcgtt
     4921 ccccaggaag acgatttccc ggacccggta tcgcgtcccc tcctcgaccc ggatcgtttt
     4981 cacgatgccg ccccggtcgt cccaggcgtt gtcgaccccc aggaccttca tccgcgcgta
     5041 cccggacttc tggtacaacc cgacgaccgc gttcatatcg tcgttccaat cctcgtcacg
     5101 gtattgcccg gaggtcgtga tccaatggaa caccccgcgg cccctcgtcg tcatctgccc
     5161 gcgaagggtc ttctcgctca aaccccgatt cccggagaaa cggatgtcct tgatgtagcc
     5221 gcgttttcct tccacgaccg acacgataag aggtgtccgg cccgcggacg gctcctccac
     5281 ggtgacggtg gcgtcgaaca gcaggtagcc ccgttcccgg taaaacgcga ccaaccgctc
     5341 ccgcagatcg cgcaccaggg cgccctcgga gatctcctcg tctccccgga gtccgccgac
     5401 ctccgaaaga cggtccggcg tgaaggcggc gattccctcc caacgcacgt cgtaccgcgg
     5461 cccctcctcc acctggacgg ccgggcacag gagatccgac gacggctcgc accggtcgac
     5521 cgtgtcgctc aggtgcacgg tgaggaaccc ctcccgcttg tactcgctcc tcacgcggga
     5581 gagtcccgcc tcccaccggc ggaagtcgta cggctttccg gcttccgcgc cgagaaaccg
     5641 cgccatctct tcgggcgtga accgggtcgc ccccggaaac cggagattcc ccacggtccc
     5701 gggctctctc tcggcaaccg tgacaaggac ctttccacca ccgttttcca cgttgcaggt
     5761 caccgaaacg gaggccgtcc cccggacgaa tcccttccgc tgcagaaagg ccctcacggc
     5821 ctcctccgcg ccggaaaggt ccttttcctc gacgggggtg ccgcgcttca gccgggacgc
     5881 cgagatgatc tgcgccggcg tgagccgctc cgtccccgcc acttcgatct ccgcgaccag
     5941 tgggaacggc cgaaggaaga agaggaggtc cacctttccg tccgtctcgc gagcgaacgc
     6001 ggtaacctcc cggaagaccg atttctcgta caacccccgg atcgacgcgc ggaccccctc
     6061 cctggtgagc cgatcccccg ggcgcacctt gacgagcccc gtgagttcct cgtaggagat
     6121 caggaacggg gacgacacct ggaaagagac cgaggagatg atggggccgg agactgccgg
     6181 cgcggggggg tcggccgcga cggcggaccc acgagggaag aagaggagcg atgccgccag
     6241 gaggaacggg agaaatttac tcctttccac gaaagaagtc cttccactgt tgataccggt
     6301 aacggaactt gagatcggcg ccgagatccc cctccgttgt cgtggtggcg ctctgccatg
     6361 acccctgaag aaagacatgc tcccgaagtt tgaactcggc ggtggcggtg ctatcggccg
     6421 acgtgccgac gctcgtggcc atcgaaacgg aggcccgatc cccgaacgat ttacccacga
     6481 cgaacttcgg ttcgaacgtc ttcgtggtcg aggagtaccc cggctcgatg gcgaacttgt
     6541 caagtcccac cgtgcttcgg atcttctcgt tcaccccccc cacggtgctt ccgatggcga
     6601 ttgccgccgt gctgcccccg ccggaagagg agcccgtgcc ggaagaagcg gtattctgcg
     6661 tcgtaacgcc gaacgccagg aggctcacga tgtcggtccg gctcaacggc gggtcggacg
     6721 tgaagtccac ctccggcttc tcgaaggtac cggagaccag gacggcaata ttgtagcttc
     6781 ccttcttcgt ctcgccccgt gcgtccaggc ggggattgat cctccgtgga tcctggaagt
     6841 ccacggtggc ccgcttcagg tcgtaccggc tgccgtataa ctcgacgtac ccttcgtaga
     6901 catcgaaggt tccgaggatg atcactttgc gggtgtcccc ctcgacctgg aactctccgc
     6961 cggctttcaa gtccgccagg ttgttcttga tccggatcgt gcgatccgcg actacgttga
     7021 tgtccagccg gacgcggaat tcggattttt ccctccgcgc aacaacatcc gagagcctcc
     7081 ggctgaaatc gacgagcgct ttctcggggt acaggacccg ggtgtagcgc gcggattgca
     7141 cctcgacatc ccccgtcacc aggaggtctt ccacgggtcc gatcaactcg acgtgcccct
     7201 ggacgaccgg acgaaaatca tccgggtaag gatatcgcac gtcgaggaaa tccaccgaga
     7261 agtaaaggcg ctgtcccgcg tccatcttga gcgggacctc tccccatccg tcgatgtttc
     7321 cgcccccgct cctcgcctcg aaatgttcga atacgatctt ttcgcggctg atgaccgcgt
//
LOCUS       DASVJL010000401         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1756169, whole genome shotgun sequence.
ACCESSION   DASVJL010000401 DASVJL010000000
VERSION     DASVJL010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756169"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..2257)
                     /locus_tag="VF357_08465"
     CDS             complement(170..2257)
                     /locus_tag="VF357_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX7524222.1"
                     /translation="MLDALFKPKAVAVIGASTKELSIGNVILRNLQEYGYRGEIYPIN
                     PTAPEVRGIKAYKSLDEIPGEVDLAHIIIPAKLVPQAIEDCGKKGVKAVVINSAGFSE
                     MGEEGAKLQAAFLANARKYGVRVFGPNCQGIINTDPALKAYCNFTFTYPEPGSISVVA
                     LSGGVGALLMQGLFDLGVGMRMYASNGNACDVSIPEILRYWGDDAGCKAIVLYTEGFS
                     DPKEFLDVAREVAAKKPVLAMKAGRTVQGAKAASSHTGSLAGVDIATELIFEKTGILS
                     FGDEEEMTRAAMAFATQPIPAGNRVGIITNTGGPAVIATDVLVAAGLDVPKLSEASIA
                     RLKTTQFPQAALENPIDVVATAGGPEFRAAMDVLLDDDGVDSIFMNFVTAPFTDTHAV
                     AREIAAVSAMGKKPIVCNFMTDLSQERYRITMRILKEGGVPFYANPTAAARALGALAA
                     YGRLRRRDIGQPESPSGVDAARAASIVEQARKSGRGVLSATDVYTIFEAYGIPVAGWK
                     VVDSAAAAVAAADAIGYPVVVKVDSEAIDHKSDMGGVAVNLKDAVAVRAAVEGMRGRL
                     GTPGTLRFLVQKFLPGGRELIVGASVSRGLGHLVMFGLGGIYVEVLKDVAFKIAPVTR
                     VEAREMLASIKTKALLDGMRGEKGIDKEKVIDLILRVSHLLTDLPMIQEMDLNPILVF
                     NDAVFAVDGRIRI"
     gene            complement(2259..>2652)
                     /locus_tag="VF357_08470"
     CDS             complement(2259..>2652)
                     /locus_tag="VF357_08470"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEX7524223.1"
                     /translation="HSELFANLKSTVLASTYCSSWIFPSFSGIDPIRSMAKAYLELFI
                     VRSDRYKEKYIQEMIGKFRIDGIVYHDAKTCPYNSNSRYGLPGRVEAETGVPSLVISG
                     DLNDLRCISDEQTKTNIEAFIEQIAEGR"
BASE COUNT          425 a          872 c          883 g          472 t
ORIGIN      
        1 aagcgggaga cagggatgtc ggagcgagac gcgacggagc cgccgcccgt ccacggatgg
       61 acgggcaagg cgtcccccgc aaggggtacc ccgtgaggcg cagaccggcc aggcggtcat
      121 ggacgaccgc gaagcggtaa cgaagggaaa cggccatcga aagatgcact tagatccgta
      181 tccttccgtc gacggcgaac acggcgtcgt tgaagacgag gatcgggttg aggtccatct
      241 cctggatcat tgggaggtcg gtgaggagat gcgagacgcg cagaatcagg tcgatcactt
      301 tctccttgtc gatccccttc tctccccgca tcccgtcgag cagcgccttc gtcttgatgg
      361 aggcgagcat ctcccgggcc tcgacccggg tgaccggcgc gatcttgaag gccacgtcct
      421 tcagcacctc gacgtagatc ccgccgagcc cgaacatcac gagatggccc agcccccgcg
      481 agacgctggc gccgacgatc agctcgcgcc ccccggggag gaatttctgg acgaggaaac
      541 ggagcgtgcc gggcgttccc agccggcccc gcatcccctc aacggcggct cggaccgcga
      601 ccgcgtcctt gaggttgacg gcaaccccgc ccatgtcgct cttgtggtcg atcgcttccg
      661 agtcgacctt gaccacgacc gggtagccga tcgcgtcggc cgcggcgacg gccgcggcgg
      721 cggaatccac caccttccag ccggcgacgg ggatcccgta cgcttcgaag atcgtgtaga
      781 cgtcggtcgc ggagaggacg ccgcggccgc ttttccgcgc ctgctcgacg atcgaggccg
      841 ccctggcggc atccacgccg gacggcgatt ccggttggcc gatatcgcgc cgcctcaggc
      901 gcccgtacgc cgcgagcgcg ccgagggccc tggccgccgc cgtcgggttc gcgtagaagg
      961 ggacgccgcc ctctttcagg atccgcatcg tgatccggta ccgctcctgg ctgagatccg
     1021 tcatgaagtt gcagacaatc ggcttcttcc ccatcgcgct gaccgccgcg atctcccgcg
     1081 ccacggcgtg ggtgtcggtg aagggggcgg tgacgaagtt catgaagatg ctgtcgacgc
     1141 cgtcgtcgtc gaggagcacg tccatggcgg cgcggaactc ggggccgccg gcggtggcca
     1201 cgacgtcgat cgggttctcg agggccgcct ggggaaactg ggtcgtcttg agccgcgcga
     1261 tcgacgcctc ggagagtttc gggacgtcga ggccggcggc gacgaggacg tcggtggcga
     1321 tgacggccgg cccgccggtg ttcgtgatga tcccgacccg gttccccgcg gggatcggct
     1381 gggtggcgaa cgccatggcc gcccgggtca tctcctcctc gtctccgaag gagagtatgc
     1441 cggtcttttc gaagatcagc tcggtggcga tgtccacccc cgccagggag cccgtgtggg
     1501 aagaggccgc cttggcgccc tgaaccgtgc gtccggcctt catcgccagc accggcttct
     1561 tcgccgcgac ctcccgggcg acgtcgagga actccttcgg gtcggagaac ccctccgtgt
     1621 agaggacgat cgccttgcac cccgcgtcgt ccccccagta gcggaggatc tcggggatgg
     1681 agacgtcgca ggcgttgccg ttcgaggcgt acatccgcat gcccacgccc aggtcgaaca
     1741 gcccctgcat cagcagggcg ccgaccccgc cgctcaacgc caccaccgaa atggacccgg
     1801 gctccgggta ggtgaaggtg aagttgcaat acgccttcag cgcgggatcg gtgttgatga
     1861 tcccctggca gttcgggccg aacacccgta caccatactt cctcgcgttt gcgagaaacg
     1921 ccgcctggag ctttgcgccc tcctcgccca tctcgctgaa ccccgcggag ttgatgacga
     1981 ccgccttgac ccctttcttc ccgcaatcct cgatcgcctg tggaacgagc ttggcgggga
     2041 tgatgatgtg cgcgagatcg acctctccgg ggatctcgtc cagcgacttg tacgccttta
     2101 tgccgcggac ctccggagcg gtggggttga tcgggtagat ctcccccctg tagccgtact
     2161 cctgaaggtt ccggaggatg acgtttccga tcgacagctc cttggtggag gcgccgatga
     2221 cggcgaccgc tttcggcttg aacagcgcgt ccagcatctt atctgccctc cgcgatctgt
     2281 tcgatgaacg cctcgatgtt cgttttcgtc tgctcgtcgg agatgcagcg gaggtcgttg
     2341 aggtcgccgg agatgaccag cgaggggacg ccggtttcgg cctccacccg tccgggcagg
     2401 ccgtaccggc tgttcgagtt gtaggggcac gtcttcgcgt cgtggtagac gatcccgtcg
     2461 atgcggaact tcccgatcat ctcctggatg tatttctcct tgtaccggtc ggagcggacg
     2521 atgaacagct cgaggtacgc cttcgccatg cttcggatcg ggtcgatccc gctgaaggac
     2581 gggaagatcc agctgctgca gtaggtggag gccaggacgg tcgacttgag attcgcgaac
     2641 agctcggagt gc
//
LOCUS       DASVJL010000402         9765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_255162, whole genome shotgun sequence.
ACCESSION   DASVJL010000402 DASVJL010000000
VERSION     DASVJL010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255162"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /locus_tag="VF357_08475"
     CDS             <1..1681
                     /locus_tag="VF357_08475"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX7524224.1"
                     /translation="DFTTRDPGLPPAAAFEENGGMSKPLRLALAQVNATVGDIAGNVR
                     KVLSACGRAREAGANLVVFPEMVLPGYPPEDLLLRPSFVEENLAAWREVAGSARGVTI
                     VAGFVDRDGKGRVWNAAGVAADGRVLGVYRKMHLPNYGVFDEMRYFHRGTEPGIFPVG
                     DARVGITICEDLWVRRGPVAREAKEGANLILNISASPYHAGKWEERRDLVAAHARRTG
                     LPVAYCNLVGGQDELVFDGGSMVVAPSGEVIARAAMFSEELLLCEIGGSPEGGLVAPI
                     PRIEEEIFRALVMGTRDYVEKNRFSGVIIGLSGGIDSSLVAAVAAEALGPSRVLGVTL
                     SSPFTSSESVEDAHALASNLGIRRLDLSIGDAFEAFGRTLAEAFAGRAADTTEENLQA
                     RIRGTILMALSNKFGEIVLTTGNKSEMSVGYATLYGDMAGGFAVIKDVFKTMVYRVSR
                     WYNESRGRDGIPDRVLTKPPSAELRPDQKDSDSLPEYDLLDPILKMYVEEDKSVPEMV
                     AAGYPEEVARRVVTLVDRSEYKRRQAPPGVKITPRALGKDRRMPITNRSNG"
     gene            1826..2029
                     /locus_tag="VF357_08480"
     CDS             1826..2029
                     /locus_tag="VF357_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7524225.1"
                     /translation="METGTVKWFNDAKGFGFISQENGGPDVFVHFSAIKSEGFKSLAE
                     GDKVQFEITQGPKGPQASNVAKA"
     gene            2293..2694
                     /locus_tag="VF357_08485"
     CDS             2293..2694
                     /locus_tag="VF357_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524226.1"
                     /translation="MGPWIPQIDDVLTAVISLACRGGEQLRVPRVCSALSELKAREVL
                     LSGLYFSITGAVCYSRQVEEILRSLMEKGMLVTKERDTLVVRDQAAEEVRAGLRKRLP
                     SSTYRALQTASKRFYRAMFKKRAGLGDPLTG"
     gene            3007..4794
                     /locus_tag="VF357_08490"
     CDS             3007..4794
                     /locus_tag="VF357_08490"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX7524227.1"
                     /translation="MKAYVKFVGWLSIAGALLLFPPDSEAGPLGAIRVNLVQGDVQVK
                     IADTGEWVPASVNMPLVEGDELWVPEGSRAALQTNRGDYVRLDEVTALQILRMDLDSY
                     QFHLAEGSVYVLNRAPKRSVLQFDTPDASIRAFGNATFRIDIPGGETDVSVFRGSVQT
                     ENGDGTTMVRAGDMLAVGAGGYAELSPVPPPDDWQRWNAQRDRIVLARGKSYGYLPEE
                     LRAYSSDFDGSGGWVSVPEYGYCWTPTVVVSDDWAPYRNGRWVWRGGDYVWIGYEPWG
                     WAPYHYGRWAFAANVGWIWVPPARGDVYWSPGYVGWVRTGDHVAWLPLAPRETYYGYG
                     NHGRYSRNITNVNVNEVRVTNVYRNANVTNSITVVNQTTFITGRPSSVDRNVVVNVRE
                     DFAKHRNIVVGRPPIKSVETSYIPVVRSIPEAKRPPAAVRKIDAKELRQSRPLVTEPN
                     RSAFRPQGKPAPLEVRKVEKPKPASERAKERQQVLPQERRGPAASPGSPPGMVEQRTT
                     PREAEPKAKGKPERVQPPPEKVKPEPAQRGGPPGTVEQRTTPKEAKPEPKAKPEPKPE
                     PKVKPERPQRPPAEKGRPDDQEEERGNRR"
     gene            complement(4840..5850)
                     /locus_tag="VF357_08495"
     CDS             complement(4840..5850)
                     /locus_tag="VF357_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX7524228.1"
                     /translation="MPTLPTLAAGCILASLVLYALTGGADFGGGVWELLNHGSRAEAR
                     KRLIVHAIGPVWETNHVWVIVAVVVLFTAFPKAFAVLSTALFVPLTLILAGIILRGSA
                     FAFHSHRLQEEGETDWWGGVFAGASLVTPLLLGVVLGAVSTGAIRGKELSSIAGGPGA
                     WLSPFPLSVGGMTLASFAYLAAVYLILETDDHELKEDFRRRALGALAVVALFSAAIPF
                     LSLGGAPEFHRALTGSWWSVPLMGANALAALGSAVSLAVRAYPLARACAASQVTLLLA
                     GWGMAQYPYLVRPDLPISSSAASPEMLRLLLIILAAGGVFLFPAIYFLFRVFKQKALF
                     GR"
     gene            complement(5854..7197)
                     /locus_tag="VF357_08500"
     CDS             complement(5854..7197)
                     /locus_tag="VF357_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX7524229.1"
                     /translation="MAELDLARAQMAMLFAFHILFAVAGIGMPVLMVIAEGAHLRTGK
                     PVYAELARRWARVTAVLFAVGAVSGTVLSFELGLLWPRFMEFAGGVIGLPFALEGFAF
                     FAEAIFLGIYVYGWEKVSPRLHLAAGCLVALGGILSGVFVVSANAWMNSPAGFTILGG
                     RPVDVSPLAAMANPSWKTEAIHMTLAAFAASGFAVAGIHAFRLLREPGNRIHRVAIVI
                     ALSVGGVAAVLQPLSGDLSARVVARNQPAKLAALEGQFRTQRGAPLRIGGLPDAAAET
                     TRYSIEIPGALSLLAFHDRDAEVVGLSAFPRDERPNPVPVHLAFQFMVACGMAMIAVG
                     AAGAILRWKGRERFFSRPFLRAVAACGPLGLLAVEAGWVVTELGRQPWIIHKVMRTSA
                     AVTPVGGLALPFAFFTLIYLALGVFAVRLLRRELAACPFFPAGEGDSPTVPGGEV"
     gene            7483..8046
                     /locus_tag="VF357_08505"
     CDS             7483..8046
                     /locus_tag="VF357_08505"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524230.1"
                     /translation="MAAGTVIAELAGIGVDLSALAAKAGDVPIKRGKEVASICPYCAV
                     GCGQIVMVDTATGKILDIQGNPDSPISQGTLCPKGTATYQLAVNPLRWTKVKYRAPLS
                     DRWEEKPLDWAMERIAQLVKKTRDANFNEFQEMPDTKGGTARKRVMNTYAIASLGGAT
                     MDNEWNYLHQKLMHALGVVSVENQARV"
     gene            8068..>9765
                     /locus_tag="VF357_08510"
     CDS             8068..>9765
                     /locus_tag="VF357_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7524231.1"
                     /translation="MGVSFGRGGSTTFPRDLVHSDAVLIMGSNMAECHPVAFRWPLKA
                     KTEHGAVLMHVDPRFTRTSALCDIHAAIRAGTDIVFLGAIINHVIHSERWNTDPFFRE
                     YVVNYTNAATLIAPDFKDTEDLDGLYSGLSPDGKSYSNATWAYQREKPPAPATKEAKT
                     FSDLLLQRIPGRPKTDPTLRNPRTVFRILERHYSRYTPETVEKICGCPKEKFLKVAGS
                     LLDNSGRDRTSNITYAVGWTQHTVGVQIIRTAAVLQSLLGNIGRPGGGVLALRGHASI
                     QGSTDIATLYHSLPGYLNMPDARQAHESLKDYILTEAGPLSTSYWGNYPKFAVSYFKA
                     QFGDAATRENDYGYDHHPKITGDHSHLPMVIDMAAGKIKGFLVMGQNPAVGGQNARFQ
                     RKAFAKLDWMVVRDFFETETASFWRNSPEIRSGELKASGIKTEVFFLPAAVVAEMEGT
                     FTNTQRLIQAHDKAVDPPGDARSDSWFSFLLGKRLKELYKDSTNDRDWAIRNLLWNYE
                     PDAAEAAEWRIKDEPSAYKLLKEINGYTSEDRKPLPTFANLNEDGSTACGAWIYTGVI
                     "
BASE COUNT         1930 a         3056 c         3282 g         1497 t
ORIGIN      
        1 cgattttacc acacgagatc cggggcttcc ccctgcggcc gctttcgaag agaatggcgg
       61 tatgagcaag ccgctgcgcc tcgccctcgc acaggtcaac gccaccgtcg gagacatcgc
      121 ggggaacgtc cggaaggtcc tttccgcctg cgggcgcgcc cgggaggccg gtgcgaacct
      181 ggtcgtcttt cccgagatgg tcctccccgg ctacccgccg gaggacctgt tgctgcggcc
      241 ttccttcgtg gaggagaatc tcgcggcgtg gcgggaggtg gcggggagcg cccgcggcgt
      301 gacgatcgtg gccggattcg tcgaccggga cgggaaagga cgggtgtgga acgccgccgg
      361 cgtggcggcg gacgggcggg tactgggcgt ctaccgcaag atgcaccttc ccaactacgg
      421 cgtcttcgac gagatgcgct acttccaccg ggggacggag ccggggattt tcccggtcgg
      481 cgacgcccgc gtcgggatca cgatctgcga ggacctctgg gtgcgccgcg gaccggtggc
      541 gcgggaggcg aaggaggggg cgaacctgat cctcaacatc tcggcgtcgc cgtaccacgc
      601 ggggaagtgg gaggagcggc gggacctggt ggcggcgcac gcgcgacgga ccggcttgcc
      661 ggtggcctac tgcaacctgg tgggaggcca ggatgagctg gtcttcgacg gcgggagcat
      721 ggtggtcgcg cccagtggcg aagtgatcgc gcgggcggcg atgttttccg aggaactgct
      781 cctgtgcgag atcgggggat cgcccgaggg ggggcttgtg gccccgatcc cgcggatcga
      841 ggaggagatc ttccgggcgc tcgtgatggg gacgcgcgac tacgtggaga agaaccggtt
      901 ctccggggtg atcatcgggc tgtccggagg gatcgattca tcgctggtgg cggcggtggc
      961 ggcggaggcg ctcggtccct cccgggtgct cggagtgacg ctgtcgtccc cgttcacctc
     1021 gtcggagagc gtcgaggacg cgcacgcgct ggcctcgaat ctcggcatcc ggcgcctcga
     1081 cctctcgatc ggcgacgcgt tcgaggcgtt cgggcgcaca ctggcggagg cgttcgcggg
     1141 ccgggcggcg gacacgacgg aggaaaatct ccaggcgcgc atccgcggta cgatcctcat
     1201 ggccctctcc aacaagttcg gggagatcgt tctgaccacc gggaacaaga gcgagatgag
     1261 cgttgggtac gcgacgctct acggcgacat ggccggcggt ttcgcggtca tcaaggacgt
     1321 cttcaagacg atggtgtacc gcgtgtcgcg ttggtacaac gagagccgcg ggcgggacgg
     1381 gatcccggac cgggtgttga ccaagcctcc ctcggcggaa cttcggcccg accagaagga
     1441 ctccgactcc cttcccgagt acgatctcct cgatccgatc ctgaagatgt acgtggagga
     1501 ggacaagagc gttccggaga tggtggcggc ggggtacccg gaagaggtcg cgcggagggt
     1561 cgtgacgctg gtggaccgga gcgagtacaa gcgccgccag gccccccccg gcgtcaagat
     1621 caccccccgc gccctgggca aggaccgccg gatgccgatc acgaaccggt cgaacggata
     1681 gggaaggggg atacaagtcg cacggtgacg ttgccttaaa taattgttgt acctattgtc
     1741 gtttcgattg tgctaatatt cagattctgg tcgatttggg ccttgttgcc tgggttgagc
     1801 aagaacattc ttttggggga ctgttttgga gacaggtaca gtgaagtggt tcaatgacgc
     1861 aaagggattc ggattcatca gccaggaaaa cggtgggccg gatgtgttcg ttcacttcag
     1921 cgccatcaag tcggagggct tcaagagcct cgcggaaggc gacaaggtcc agttcgagat
     1981 cacccagggt cccaagggcc ctcaggcttc gaacgtcgca aaggcctgat cacgccggac
     2041 gacctcgggt aaagaacccg ggaatgatga aacccccggc gggtatcctg ccgggggttt
     2101 tttatccatg cgaggaatac gatattcgta tcctcccttc cactcttgta attccgaacc
     2161 ttcctgcgga tcgcttgcga tagaataata tcgtccgttc ctcctttttc gcggtaatgt
     2221 cgaggcatgc ggattgcttg tctcacttta tcgaccgaag cgagtccacg aatcaggaga
     2281 aggagcgcct gaatggggcc ctggattccg cagatcgacg atgtcctaac cgcggtcatc
     2341 agtctcgcgt gccgcggggg cgagcaactc cgagtgcccc gggtctgttc cgcgctgagc
     2401 gaattgaagg cgagggaggt gctcctgtcg gggctctact tctccatcac gggggcggtg
     2461 tgctactccc ggcaggtcga ggagatcctg cggtcgctga tggagaaagg gatgctcgtc
     2521 acgaaggagc gcgacacgct ggtggtccgg gaccaggcgg cggaagaggt gcgcgccggg
     2581 ctgaggaaga ggctcccctc gtcgacctac cgggcgctcc aaaccgcctc gaaaaggttc
     2641 taccgggcaa tgttcaaaaa gagggcggga ctcggggacc cgctcaccgg ctgagaccga
     2701 tctctccctt cgcctttctc cgccgaaacc gaatcttcca tccgatgatg tttcccatgc
     2761 aacaaggatc accatcccgc atcaaacgac atgtggcggg aatccggccc gaacggcatc
     2821 ctgcggacgg gaagaacgac ggtgccatcg gcgtgaacgg cggcaagtcg ttggggtagc
     2881 cgggccccgg gaacctgtcg aataaaccgg ggttttcatc cgtcagaaga acagcaggct
     2941 tccgggatca tggcacaagg gaaagaatcg gaatctaacg gacccttacc gggatcggag
     3001 cgtcggatga aggcatacgt gaaatttgtc ggctggttgt caatcgccgg agcgttgctg
     3061 ctgttccccc cggattccga ggcaggtccc ctcggggcga tacgggtcaa tctcgtccaa
     3121 ggggacgtgc aggtgaagat cgccgatacg ggagagtggg tgcccgcctc ggtcaatatg
     3181 ccccttgtcg agggggacga gttgtgggtc ccggaaggga gtcgcgcggc gctccagaca
     3241 aaccgtggag actatgtccg gctcgacgag gtcacggccc tgcagatcct gcggatggac
     3301 ctcgactctt accagttcca cctggcggag gggagtgtgt atgtgctgaa ccgcgccccg
     3361 aaacgcagcg tcctccagtt cgacaccccc gacgcctcca tccgggcttt cgggaacgcg
     3421 accttccgga tcgacatccc cggcggcgaa acggacgtct ccgtattcag gggatcggtc
     3481 cagacggaaa acggcgacgg aacgaccatg gttcgggcgg gcgacatgtt ggccgttggt
     3541 gcgggcggtt acgcagagct atccccggtt cccccacctg acgactggca gaggtggaac
     3601 gcccaacggg accggatcgt ccttgcgcgg gggaaaagct acgggtacct gccggaggaa
     3661 ctccgggcgt actccagcga cttcgatggc agcggcgggt gggtgagtgt gccggaatac
     3721 gggtattgct ggacccccac ggtcgtcgtg agcgacgact gggccccgta ccggaacggg
     3781 aggtgggtgt ggaggggcgg agactacgtc tggatcggct atgagccctg gggatgggcc
     3841 ccctaccatt acggccgctg ggccttcgcc gccaacgtcg gctggatctg ggtgccgccc
     3901 gcgcggggag atgtctactg gtcccctgga tacgtgggct gggtgcgaac gggggaccac
     3961 gtggcgtggc tgccccttgc cccgagggaa acgtactacg gatacggcaa ccacggacgg
     4021 tacagcagga atatcacgaa cgtgaacgtg aacgaggtcc gggtgaccaa cgtctaccgg
     4081 aacgcgaacg tcaccaacag catcaccgta gtcaatcaga cgaccttcat cacgggccgg
     4141 ccgtcctccg tcgaccggaa cgtggtggtg aacgtgcggg aggacttcgc aaagcaccgg
     4201 aacatcgttg tcggaaggcc cccgatcaag tccgtggaga cgagctacat cccggtggtc
     4261 cggtcgatcc ccgaggcaaa gcgcccgccg gcggctgtcc ggaagatcga cgcgaaggaa
     4321 ctaaggcaat cccgccctct ggtcacggaa ccgaaccggt ccgccttccg gccgcaaggc
     4381 aagcccgccc ccctggaggt caggaaagtc gagaaaccga agcccgcaag cgagagggcc
     4441 aaggagcggc agcaagtcct gccccaggaa cggcgagggc cggcggcatc gccagggagc
     4501 ccgccgggaa tggtggaaca gcgcaccacg ccgagggagg cggagccgaa ggcgaaagga
     4561 aagccggagc gcgtgcaacc gccgccggag aaggtaaagc ccgaaccggc gcaaaggggt
     4621 ggcccgccgg gaacggtgga acagcgcacc acgccgaagg aagcgaagcc ggagccgaag
     4681 gcgaaaccgg agccgaaacc ggagccgaag gtgaaaccgg agcgcccgca acggcccccc
     4741 gcggaaaaag gacgtcccga tgatcaagaa gaggaacggg ggaaccggcg ataggggaac
     4801 ggatgaggcc cggccgggtg gtccgccggg cacggagatt cagcgtccga agagggcttt
     4861 ctgcttgaag acgcggaaga ggaagtagat cgccgggaac aggaagacgc ctcccgcggc
     4921 caggatgatc agcagcaggc ggagcatttc aggtgaggcg gcagacgagg agatcggcag
     4981 gtccggccgg acgaggtatg ggtactgggc catcccccat ccggcgagga gaagcgtcac
     5041 ctgcgaggcc gcgcaggctc ttgccagcgg gtacgcgcgg actgcaagag agacggccga
     5101 tcccagtgcg gccaaggcat tcgcccccat caaggggacg gaccaccagc ttccggtcaa
     5161 ggcccggtgg aactccggcg ctccgccgag agacaggaag gggatggcgg cggagaacag
     5221 cgcaaccacc gccaatgcgc ccaatgcgcg gcggcggaaa tcttccttca actcatgatc
     5281 gtccgtttcg aggatcaggt agaccgcggc caggtaggca aacgatgcca gcgtcatgcc
     5341 cccgacggag aggggaaacg gtgaaagcca ggcgcccggg ccgccggcaa tggacgaaag
     5401 ttccttcccg cggatcgcac ccgtggacac cgccccgaga accacgccca gcaggagcgg
     5461 cgtgaccagg ctcgcacccg cgaagacgcc gccccaccaa tccgtctcac cttcctcttg
     5521 caggcgatgc gaatggaacg cgaaggcgga accccggagg atgatcccgg caaggatgag
     5581 cgtgaggggc acgaacagag cggtggacag gaccgcgaag gccttgggaa acgcggtgaa
     5641 caggaccacc acggccacga tgacccagac gtggttcgtc tcccagaccg gcccgatggc
     5701 gtggacgatg agtcgtttcc gggcctccgc cctggaaccg tggttcagca gttcccaaac
     5761 gcctccgccg aaatccgcgc ctccggtgag agcgtacagc acgagggacg ccagaatgca
     5821 tcccgccgcc agggtgggga gggtcggcat gcgtcacacc tccccccccg gcaccgtcgg
     5881 ggagtcccct tcccccgccg ggaagaaggg gcacgccgcc agctccctgc gcagaaggcg
     5941 taccgcgaag acaccgaggg cgaggtagat gagggtgaag aacgcgaacg gaagggcgag
     6001 gccgcccacc ggggtcacgg cagcggaagt cctcatcact ttatggatga tccacggctg
     6061 ccgcccgagc tcggtcacga cccagcctgc ctccacggcg aggagaccca gggggccgca
     6121 ggccgcaacg gcgcgaagaa acggccggga gaagaaccgc tcccgcccct tccacctcag
     6181 gatggcgccc gcagccccca cggcgatcat tgccatgccg caggcgacca tgaactggaa
     6241 cgcaaggtgg accggcacgg ggttgggccg ctcgtccctt gggaaggcgg acaacccgac
     6301 gacctcggcg tcccggtcgt ggaaggccag gaggcttaag gcccccggga tctcgatgga
     6361 gtagcgggtg gtttcggccg ccgcgtccgg cagcccaccg atccgcaacg gtgcaccgcg
     6421 ttgcgtccgg aactgccctt ccagcgcggc tagttttgcg ggctggttcc gggccacgac
     6481 gcgggcgctc aagtctccgc tgaggggctg aagcaccgcg gccaccccgc cgaccgaaag
     6541 ggcgatgacg atggccacgc ggtgaatgcg gtttcccggc tcccgcagca ggcggaacgc
     6601 atggatcccc gcgacggcga acccggaggc ggcgaaggcg gccaacgtca tgtggatcgc
     6661 ctcggtcttc caggagggat tggccatcgc cgccagcgga gagacgtcca ccggccttcc
     6721 ccccaggatc gtgaacccgg cggggctgtt catccaggcg ttggcggaca ccacgaacac
     6781 ccctgagaga atccctccca gggccaccag gcaccccgcg gcgaggtgca gccgggggga
     6841 gaccttctcc cagccataga cgtagatccc gagaaagatc gcctcggcga agaaggcgaa
     6901 tccttcgagc gcgaagggca gtccgatgac cccgccggcg aattccatga aacgcggcca
     6961 gagaaggccg agctcaaagg agaggaccgt ccccgagacg gcgccgacgg cgaagaggac
     7021 cgcggtgacc cgggcccacc ggcgggccag ctcggcatac accggcttgc cggtcctgag
     7081 gtgggcgcct tccgcaatga ccatcaggac cggcatcccg atccccgcca ccgcgaagag
     7141 gatgtggaag gcgaagagca tcgccatctg ggctcgcgca aggtccagtt cggccatcga
     7201 tccccttcga aactccgctg attttttcca ttgtattccg ggagggcgtc cattgccagt
     7261 cggaagggat acgccccatg gattcctcgg cgatgagggt gcccgcatta tcggctctgt
     7321 ttattaattt tattttggta ttatttacca acaacacacg acgattcatg gatacaacgg
     7381 caatcgattt gacccgttcc atcacgggaa ggagagtcga cgattcctga ttccccggaa
     7441 gtgaacccgt ctcgacgtat cttcctcaaa ggggccgccg gtgtggcggc cggcaccgtt
     7501 attgccgagc ttgccggaat cggcgtcgac ctgagcgcct tggccgccaa agcgggggat
     7561 gtgccgatca agagagggaa ggaggtggca agcatctgtc cgtactgcgc cgtcggctgc
     7621 gggcagatcg tcatggtaga caccgctacg gggaagatcc tcgacatcca ggggaacccc
     7681 gattccccga tcagccaggg caccctctgc ccgaagggga ccgccactta ccaactcgca
     7741 gtgaatcccc tgcgctggac gaaggtgaag taccgggctc ccctgagcga ccgttgggaa
     7801 gagaagcccc ttgattgggc gatggagcgc atcgcacagc tcgtcaagaa gactcgggac
     7861 gcgaacttca acgagttcca ggagatgccc gacaccaagg gcgggacggc ccgcaagcgc
     7921 gtgatgaaca cctacgccat cgcgtcgctc ggcggggcca cgatggacaa cgagtggaac
     7981 tacctccacc agaagctcat gcacgccctg ggggtcgtat ccgtcgaaaa ccaggcacgg
     8041 gtatgacatt cctccacggt ccccggtctg ggggtctcct tcggtagagg cggttccacc
     8101 accttccctc gcgacctcgt ccactccgac gcggttctga tcatgggctc caacatggcg
     8161 gaatgccatc cggtggcctt ccgctggccg ctcaaagcca agacggaaca cggcgccgtc
     8221 ctcatgcacg tggacccgcg cttcacgcgc acgtccgccc tttgcgacat ccacgccgcc
     8281 atccgggcgg gaaccgacat cgtgttcctc ggggcgatca taaaccacgt gatccacagc
     8341 gagcgctgga acaccgatcc gttcttcagg gagtacgtgg tcaactatac caacgcagct
     8401 acattgatcg cccccgactt caaggacacc gaagacctgg acgggctcta ctccgggctt
     8461 tcccccgacg ggaagtcgta cagcaacgcc acgtgggcct accagcgcga aaagccgccg
     8521 gccccggcga caaaggaggc gaagaccttc agcgacctcc tgctgcagcg aatcccgggg
     8581 cgccccaaga cggacccgac gcttcggaac ccgcgcaccg tctttcggat cctggaacgg
     8641 cactactccc ggtacacacc cgagacggtg gaaaagatct gcggctgtcc gaaggaaaaa
     8701 ttcctgaagg tggccgggtc cctgttggat aactccggcc gcgaccggac gtcgaacatc
     8761 acctacgccg tggggtggac gcagcacacg gtgggcgtgc agatcatccg caccgccgcc
     8821 gtcctgcaat cactgctcgg caacatcggg cgacccggag ggggggtgct cgcccttcgc
     8881 ggccacgcat cgatccaggg ctccaccgac atcgccacgc tgtatcactc ccttcccggc
     8941 tacctgaaca tgcccgatgc gcgccaggcg cacgaatcct tgaaggacta catcctcacc
     9001 gaggcggggc cgctttccac cagctactgg gggaactatc ccaagttcgc cgtctcctat
     9061 ttcaaggccc agttcgggga cgcggcgacc cgggagaacg actacgggta cgaccatcat
     9121 cccaagatca ccggcgacca ttcccacctg ccgatggtca tcgacatggc ggcgggaaag
     9181 atcaaggggt tcctggtgat ggggcagaac cccgccgtgg gcgggcagaa cgcccgcttc
     9241 cagcggaagg ccttcgcgaa gctcgactgg atggtggtcc gcgatttctt cgagacggag
     9301 acggcctctt tctggcgcaa ctcccccgag atccggtcgg gggagctgaa ggcctccggg
     9361 atcaagaccg aggtgttctt cctccccgct gccgtcgtcg cagagatgga agggaccttc
     9421 accaatacgc agcggctgat ccaggctcac gacaaggccg tcgatccgcc cggcgacgcg
     9481 cgcagcgact cctggttcag cttcctcctg ggcaagcgac tcaaggagct gtacaaggat
     9541 tcgacgaacg accgcgactg ggcgatccgg aacctgttgt ggaactacga gccggatgcg
     9601 gccgaagcgg cggagtggcg gatcaaggac gagccctccg cctacaagct cctgaaggag
     9661 atcaacggct acacctcgga ggacaggaag ccgcttccca cgttcgccaa cctgaatgag
     9721 gacggctcca cggcctgcgg cgcctggatc tataccgggg tgatt
//
LOCUS       DASVJL010000403         4569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1569704, whole genome shotgun sequence.
ACCESSION   DASVJL010000403 DASVJL010000000
VERSION     DASVJL010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569704"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..775)
                     /locus_tag="VF357_08515"
     CDS             complement(20..775)
                     /locus_tag="VF357_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX7524232.1"
                     /translation="MATRVGIVILGDEVLKGEIREANLAYMIPLLAEWGAETGLCAIL
                     PDDVPVVVRHLRRFREEVDLVVLTGGIGPTPDDITRDAVAEVAGVPLIVHPEAKAALE
                     ARPYKGSNPEYRMLMAQVPQGATLIPNPLSPAPGFFIDGMAVFPGVPRMLQAMFEWVK
                     PMVSGRRKSRITLYAMAPESSYAGIMKVAMTTFPDVGIGSYPMSDGEYRVRVVFRGDR
                     FERTDACAARFTKFLSGIGYEVLRRVEERGDDA"
     gene            886..1941
                     /gene="purM"
                     /locus_tag="VF357_08520"
     CDS             886..1941
                     /gene="purM"
                     /locus_tag="VF357_08520"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX7524233.1"
                     /translation="MKKPVTYRTAGVDIDEGERLVRRIRPMVRTTHRKEVLGEIGSFA
                     GFFRFPARKYRDPVLVSGTDGVGTKVKVAAAAGKFDTVGIDLVAMCVNDILVHGAEPL
                     FFLDYYATGKLSADHGAQVVSGVAEGCRQAGCALLGGETAEMPSVYARGEFDLAGFAV
                     GVVDRAKIIDGSSARPGDQLVGLFSSGLHSNGYSLARKVVFDILRKRIGQRVPEWGAT
                     VAEELLRPTRIYVRPVLSLLRRVTILGMAHITGGGITGNVPRSVPDGVTAVVARSAWE
                     VPPVFRTICAAARLPDEEAFRTFNMGIGMVLMLRPGDADRAVAHFRRAGIPAAVIGEI
                     RKGRRRQPNVTFAGGER"
     gene            1938..2594
                     /gene="purN"
                     /locus_tag="VF357_08525"
     CDS             1938..2594
                     /gene="purN"
                     /locus_tag="VF357_08525"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX7524234.1"
                     /translation="MNDRPVIAVLVSGSGSNLQAIIDASERGEIPGRVGLVVSNKADA
                     YGLARARKHGIPTSVVDHKAFESREAFDAKLVEVIRASGAVLVCLAGFMRVVTPVFLR
                     AFPHRILNIHPALLPSFPGTHGPAQALRYGVRFSGCTVHFLDEGVDTGPIIVQAVVPV
                     HEDDTEETLAARILKEEHRIYPMAIRLFLEGRLTVEGRKVSTKDAEKIPDFSRRNPDA
                     "
     gene            complement(2608..3216)
                     /locus_tag="VF357_08530"
     CDS             complement(2608..3216)
                     /locus_tag="VF357_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX7524235.1"
                     /translation="MKTQSPLDTGPRRRRGGFFRPVLFLLVLLFLPAAIPHLLALRDP
                     GPPPTRPADAIFVLTGGEGRIQEGFRAWSGGAARELYILGAGRRIPVARIVPEASRIP
                     AEALSRVHVEGWSENTLENAFSAKSAVGEGRYSSVILVTSDYHIPRAYLAFRKVLPPD
                     VSLSAIRVRPEGGEGASWRWVRRHFIEGWKYWGYRILLRWEE"
     gene            3261..3449
                     /gene="rpmB"
                     /locus_tag="VF357_08535"
     CDS             3261..3449
                     /gene="rpmB"
                     /locus_tag="VF357_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX7524236.1"
                     /translation="MAKCAICGKVPSFGHNVSHANNRTARVWQPNIQRVKTVQGGTVR
                     HIHACTACIKSGRVVKAV"
     gene            complement(3458..3844)
                     /locus_tag="VF357_08540"
     CDS             complement(3458..3844)
                     /locus_tag="VF357_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7524237.1"
                     /translation="MKREAVRTAGAPVAIGPYSQAVRAGGFLFCSGQIPLDPSTGKMV
                     DGGIEAQAERVLKNLEAVLTTGGATLQSVVKTTVFLVDLGDFPAMNAVYGKFFPKDPP
                     ARATVQVAKLPAGARVEIDAIAVEAT"
     gene            complement(3846..>4569)
                     /locus_tag="VF357_08545"
     CDS             complement(3846..>4569)
                     /locus_tag="VF357_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7524238.1"
                     /translation="AWLANLFVKPDSAFGLQMGGSVYVDRISVAGLPEFVEVLQESRC
                     RTADDYMRTVGYGKASVMPLLLRLLPADQLHEKSKESRLSTLIRKVVTRRPSSVTVKG
                     MDSIFVRLGNCCHPVPGDPIVGFITRGRGVTIHSKECPKVLESDPARAVEVNWEAGTK
                     AVQPVKLRVVCSDKPGLLADISRSITSSEVDIRRASVMTTRDQRAICDFEVSVNDANH
                     LASLIKSIGKVRGVQSVERGKG"
BASE COUNT          770 a         1563 c         1466 g          770 t
ORIGIN      
        1 taagaggaac agcgcgatct tacgcatcat cgccacgctc ctccacccgg cggagaactt
       61 catacccgat ccccgagagg aacttcgtga agcgcgcggc gcacgcgtcc gtccgttcga
      121 accggtcgcc gcggaacacc acccgcacgc gatactcccc gtcgctcatc gggtacgacc
      181 cgatgccgac atccgggaac gtcgtcatcg ccaccttcat gatcccggcg taggacgatt
      241 ccggggccat cgcgtagagg gtgatccggc tcttgcggcg gccggaaacc atcggcttca
      301 cccattcgaa catcgcctga agcatgcgcg ggacgccggg gaagaccgcc atcccgtcga
      361 tgaagaagcc gggagccggg gagagcggat tcgggatcag tgtcgcgcct tgcggaacct
      421 gtgccatcag catccggtat tccggattcg accccttgta cgggcgggct tccagcgccg
      481 ccttcgcctc cgggtggacg atcaacggga ccccggcgac ctcggccacc gcatcccggg
      541 tgatgtcgtc gggagtcggc ccgatccccc cggtcaggac gaccagatcg acctcctcgc
      601 ggaaccggcg caggtgccgc accacaacgg gaacatcgtc cggaaggatc gcgcacaacc
      661 ccgtctccgc cccccactcg gccagcaacg ggatcatgta ggcgaggttc gcctccctga
      721 tctccccttt cagcacctcg tcgccgagga tgacgattcc cactctcgtc gccatgcatc
      781 cctccctgtt cgaatctttt cattttaccc cgtgacgggg ggaaaatcat tgacatcccc
      841 tttggcgaat cgttaccgtg gggtacattc cttccggagc gcatcttgaa aaaacccgtg
      901 acgtacagaa ccgccggtgt cgacatcgac gagggggaac gcctcgtgcg ccggatccga
      961 ccgatggtcc gcacgacgca ccgcaaggag gtgctggggg agatcggctc gttcgcgggc
     1021 ttcttccgct tccccgcgcg gaagtatcgc gacccggtcc tcgtctcggg caccgacggc
     1081 gtggggacca aggtcaaggt cgctgcggcg gccgggaagt tcgacacggt agggatcgac
     1141 ctcgtcgcca tgtgcgtcaa cgacatcctc gtccacggcg ccgagccgct cttcttcctc
     1201 gattactacg ccaccggaaa gctttcggcc gaccacgggg cccaggtcgt atcgggcgtc
     1261 gcggaggggt gccggcaggc cggctgcgcc ttgctcgggg gagagaccgc ggagatgccg
     1321 tcggtctacg cccggggaga gttcgatctc gcgggattcg cggtgggagt ggtggaccgg
     1381 gccaagatca tcgatggcag ctccgcgcgc cccggcgacc agctcgtggg tcttttttcc
     1441 tccggcctcc acagcaacgg gtactccctg gcgaggaagg tcgtcttcga cattctccga
     1501 aagcggatcg gccagcgcgt ccccgagtgg ggcgcgacgg tcgcggagga gcttctgcgc
     1561 cccacccgca tctacgtgcg ccccgtcctt tccctgctcc ggagggtgac gatcctcggt
     1621 atggcccaca tcaccggggg ggggatcacc gggaacgtcc cgcgttccgt gccggatggc
     1681 gttacggcgg tcgtcgcccg gtccgcatgg gaagtcccgc ccgtcttccg cacgatctgc
     1741 gcggcggcgc gccttcccga cgaagaggcg ttccgcacct tcaacatggg gatcgggatg
     1801 gtgctcatgc tccggccagg ggacgcggat cgggcggtcg cccatttccg gcgcgccggt
     1861 attccggccg ccgtgatcgg ggagatccgg aaggggcgcc gccgacagcc gaacgtgacc
     1921 tttgcgggag gtgaacgatg aacgaccgac cggtcatcgc cgtcctcgtc tcggggagcg
     1981 gatccaactt gcaggcgatc atcgacgcct cggagcgggg ggagatcccc gggcgggtcg
     2041 gcctggtcgt gagcaacaag gcggacgcct acggccttgc gcgggcgagg aagcacggga
     2101 tcccgacctc ggtggttgac cataaggcgt tcgagagcag ggaagcgttc gacgcgaaac
     2161 tggtggaagt gatccgtgcc tcgggggccg tcctcgtctg ccttgccggc ttcatgcggg
     2221 tggtgacgcc ggttttcctt cgcgcgttcc cgcaccggat cctcaacatc cacccggcgc
     2281 tgctcccctc cttccccggg acccacgggc cggcgcaggc gctgcggtac ggcgtccggt
     2341 tctcggggtg taccgtccac ttcctcgacg agggggtcga caccgggccg atcatcgtgc
     2401 aggcggtcgt cccggtgcac gaggacgaca cggaggagac gctcgccgcc cgtatcctca
     2461 aggaggagca ccggatctac ccgatggcga tccggctgtt tctcgagggg aggctcaccg
     2521 tcgaggggag aaaggtgtcc acgaaggacg cggaaaagat ccctgacttc tcccggcgca
     2581 accccgacgc ctgaatccgt ccctccctca ttcctcccac cggaggagga tccggtaccc
     2641 ccaatatttc cacccctcga tgaagtgccg tctcacccaa cgccaggagg cgccttcgcc
     2701 cccctcggga cgcacccgga ttgcggagag cgacacatcc ggcggaagga cctttcgaaa
     2761 ggcgagatac gcgcggggga tgtggtagtc ggaggtcacg aggatgacgg aggagtatct
     2821 tccctctcct accgcggatt tcgcggaaaa ggcgttttcg agcgtgttct cggaccaccc
     2881 ttccacgtgg acgcgcgaga gcgcctcggc ggggatccgc gaagcttcgg gcacgattcg
     2941 cgcgacgggg atcctccgtc ccgcaccgag gatgtacagt tccctcgccg cgccgccgga
     3001 ccatgcgcgg aatccctcct ggatcctccc ctcccctccg gtgagcacga agatcgcgtc
     3061 cgcggggcgc gtgggggggg gaccgggatc ccgcaacgcg aggaggtgag ggatcgccgc
     3121 agggagaaag aggaggacga gcagaaagag gacgggccgg aagaatcctc ccctgcgtcg
     3181 gcgaggaccg gtatcgaggg gggattgtgt tttcatggcg tccgtgatag aaaatatggt
     3241 ttgaatttac ggaggaactc atggccaagt gcgcaatctg cggtaaggtc cccagcttcg
     3301 ggcacaacgt ctcccacgcc aacaaccgta ccgcaagggt atggcagccg aacatccaac
     3361 gtgtgaagac ggtccagggc gggacggtgc gtcacatcca cgcctgcacc gcgtgcatca
     3421 agtccggccg ggtcgtcaag gcggtctgac ccgagtttca tgtagcctcg accgcgatcg
     3481 catcgatctc gacacgcgcc cccgcgggca gtttcgcgac ctgcaccgtc gcccgcgccg
     3541 gcgggtcctt tggaaagaac ttcccgtaga ccgcgttcat cgcagggaaa tcccccagat
     3601 cgacgaggaa gacggtggtc ttcacgaccg actggagggt tgcgcctccg gtggtcagca
     3661 ccgcctcgag gttcttcagg acccgctccg cctgggcctc gatcccgccg tccaccattt
     3721 tccccgtcga cggatcgagc gggatctgcc ccgagcagaa gaggaacccg cctgcccgca
     3781 ccgcctggga gtagggaccg atcgccaccg gggcccccgc cgtccgcacc gcttcgcgct
     3841 tcatctcacc ctttccccct ctccaccgat tgtacaccac ggaccttccc gatcgacttt
     3901 atgagggagg ccaggtggtt ggcgtcgttc accgacacct cgaaatcgca gattgcgcgt
     3961 tggtcgcggg tcgtcatcac cgacgccctc cggatgtcca cctcgctcga cgtgatgctc
     4021 cgggagatgt cggccagaag ccccggcttg tcggagcaca cgacgcgcag cttgacgggc
     4081 tggaccgcct tcgtccccgc ctcccagttc acctcgaccg cccgcgccgg atcgctctcg
     4141 aggactttcg ggcactcctt cgaatggatc gtgacccccc gtccccgggt gatgaacccg
     4201 acgatcggat cgcccggcac cgggtggcag cagtttccca ggcgtacgaa gatgctgtcc
     4261 atccccttca cggtgacgga gctgggcctg cgcgtcacca ccttccggat gagggtggac
     4321 aggcgcgact ccttgctctt ctcgtgcagc tggtccgcgg gaaggaggcg gagcagcagc
     4381 ggcatgaccg aggcctttcc gtatccgacc gtcctcatgt aatcatccgc ggtcctgcag
     4441 cgggactcct gcagcacctc gacgaactcg ggcagccccg ccacgctgat ccggtcgacg
     4501 tacaccgatc cgcccatctg gagtccgaac gcgctgtcgg gcttcacgaa cagattggcc
     4561 agccacgcg
//
LOCUS       DASVJL010000404         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1569765, whole genome shotgun sequence.
ACCESSION   DASVJL010000404 DASVJL010000000
VERSION     DASVJL010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569765"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VF357_08550"
     CDS             complement(<1..707)
                     /locus_tag="VF357_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7524239.1"
                     /translation="MAPFAFLDLFNPVRWFRRNAVFHTVHSTERFRDLIERERSRADR
                     TGICFSLVVFNHKGRKRDTYRTLYHLVGLLTSRARTSDEIGWFDKSSLGVIMIGAGEA
                     GATEFGRKVTASLPEGVAVPKVLVYIYPTNGQGEGEPGSAVEPGSEAGTFQGFSPPTV
                     TENAALPEDAAMPSAFFRQLPLWKDILDVVVSLIALILLSPLFLLIAVYIKIADAGPV
                     FFRQGRIGFRGKKFEGW"
     gene            complement(710..1483)
                     /locus_tag="VF357_08555"
     CDS             complement(710..1483)
                     /locus_tag="VF357_08555"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX7524240.1"
                     /translation="MIKLPFFRKNGKPPGARRPIDLFAVGKEDPLYNEKFKGLRAMVE
                     QKADLHQMRLLGITSSIAGEGKTVTCAHLGRSLASTGRKKVLLVDVDIRKADLTHGMG
                     ARRSPGLTEYLLGRDTIQQFIRWTHIPNLSLITSGIEVNSPADLLAGDKFKSFLQEIR
                     TSYDIILLDTPPILPVADTPTFRDLTDGFIFVYRAGFTPYTMLRQAMEELDEKNVLGA
                     VLNGVEVASDSYYQKYYGGYYHAKADSVNDRTAEKEAIR"
     gene            complement(1480..>2559)
                     /locus_tag="VF357_08560"
     CDS             complement(1480..>2559)
                     /locus_tag="VF357_08560"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEX7524241.1"
                     /translation="QEERVKQYKLKFMGELPQQLQANLQILSRLQEQLKANSDATRNA
                     QDRKVYLEAQIGVLESQSNAFAAQMSAAGKAAREAAAAGSDAPVSSGTSTAALANELS
                     VKKSQLAVLSGKYTEKYPEIRRLKDEVAQLEKRLAEAIAQEGPSGSVSQAPGSTGRDE
                     ILRLRAQRKALDSEIASLSKDRQGIEKTIASLEARVEKSPRREQEMVSLTRDYENLKA
                     SYDDLLKKKLDAEVSQNLEKRQKGEQFQILDPADLPQKPFTPDRPKVFGIAFAAAMLI
                     GFGGAIGLEMINPTLRGKRDFQHFFQVPVLASIPVIRDTRYEARKSRQMMVVYSGLIS
                     FGALVTLFLVVFGQKVRNLLLGMFS"
BASE COUNT          464 a          786 c          791 g          518 t
ORIGIN      
        1 ttccagcctt cgaatttttt cccccggaaa ccgatccggc cctggcggaa gaataccggc
       61 ccggcgtccg cgatctttat atagacggcg atcagcagga agagcgggga aaggaggatc
      121 agcgcgatca gggagaccac cacgtcgaga atgtccttcc agagcggcaa ctgccggaag
      181 aatgccgacg gcatcgccgc atcttccgga agggcggcgt tctcggtcac ggtgggcggg
      241 gaaaatccct gaaacgttcc cgcttccgat ccaggttcca ccgcagatcc gggttccccc
      301 tcgccttgac cgtttgtcgg gtagatgtac accaagacct tcgggacggc gactccctcc
      361 ggcagggagg cggtcacctt ccgcccgaat tcggtggcgc cggcttcccc tgccccgatc
      421 attatgacgc ccagggagga cttgtcgaac cagccgatct cgtcgctggt gcgggcgcgg
      481 gaggtcagca ggccgaccag gtgatacagc gtgcggtagg tgtcccgttt ccttcccttg
      541 tggttgaaca ccaccaacga gaagcagatc cccgtgcggt cggcgcggga acgctcccgc
      601 tcgatgagat cacggaaccg ttccgtggag tggaccgtat ggaacacggc attccgccgg
      661 aaccagcgga ccggattgaa cagatcgaga aaggcgaacg gagccatcgc taccggatcg
      721 cctccttttc cgccgtccga tcgttcacgg aatccgcttt tgcgtggtaa tagccgccgt
      781 aatatttctg gtaatacgaa tcgctcgcca cctccacccc gttgaggacg gcgccgagta
      841 cattcttctc gtcgagttcc tccatcgcct ggcggagcat cgtgtagggg gtgaagccgg
      901 cccggtagac aaagatgaat ccgtccgtca ggtcccggaa cgttggagta tcggcgacgg
      961 gaaggatggg gggagtgtcg aggaggatta tgtcgtacga tgtgcggatc tcttgcagga
     1021 atgacttgaa cttgtccccc gccagcaggt ccgccggaga attcacctcg atgccggaag
     1081 tgatcaggga cagattcgga atatgggtcc aacggatgaa ctgctggatc gtgtcccttc
     1141 caagcagata ttcggtcagg cccggagagc ggcgggcgcc catcccgtgg gtcagatccg
     1201 ccttccggat gtccacatcc accaggagga ccttctttct cccggtcgat gcgaggctcc
     1261 tgccgagatg ggcacaggtg accgtcttcc cctcgccggc gatcgaactg gtgattccaa
     1321 gaagcctcat ctggtgaagg tccgccttct gctccaccat ggcccggagc cccttgaact
     1381 tctcgttgta cagcggatcc tctttcccaa ccgcgaagag gtcgatcggg cggcgcgcac
     1441 cgggcggctt gccgttcttc cggaaaaacg ggagcttgat catgagaaca tccccaggag
     1501 gaggttgcgg accttttggc cgaagacgac gaggaaaagg gtgaccaacg cgccgaagga
     1561 gatcaggccg ctgtaaacaa ccatcatttg ccggcttttt cgggcttcat atctcgtgtc
     1621 gcggatgacc gggatcgagg cgagcaccgg gacctggaag aagtgctgga agtcccgctt
     1681 cccgcgcaat gtgggattga tcatctcgag cccgatggcc ccgccaaacc cgatgagcat
     1741 ggcggcggcg aaggcgatgc caaacacctt cggccggtcg ggggtgaacg gcttttgggg
     1801 caggtccgcg gggtcgagga tctggaactg ctcccctttc tgccgcttct cgagattctg
     1861 cgacacctcg gcgtcgagct tcttcttcag caggtcgtcg tacgacgctt tcaggttctc
     1921 gtagtcccgc gtcaacgaca ccatctcctg ctccctgcgc ggggatttct cgacccgggc
     1981 ctcgaggctt gcgatcgtct tttcgatccc ttggcggtcc ttgctgagcg atgcgatctc
     2041 cgaatcgagg gctttgcgtt gagcccgaag ccgaaggatc tcgtcgcgcc ccgtggatcc
     2101 gggagcctgg ctgacggaac cggacgggcc ttcctgcgcg atcgcctcgg cgagacgttt
     2161 ctccagttgc gccacttcat cctttagacg ccggatctcc gggtactttt cggtgtattt
     2221 ccccgaaagt accgccagtt gggatttttt caccgacagt tcgttcgcca gcgcggcggt
     2281 cgacgtgccg gaagagaccg gggcatcgga gccggcggcg gccgcctccc gggccgcctt
     2341 cccggcggcg gacatctggg ccgcaaaagc gttcgactgc gactcgagga ccccgatctg
     2401 ggcttccaga taaaccttcc ggtcctgtgc gttacgggtg gcgtcggaat tcgccttcaa
     2461 ctgctcctga aggcgcgaga ggatctgcag attcgcctgg agctgctggg gcaactcccc
     2521 catgaacttc agcttgtact gcttgaccct ttcctcttg
//
LOCUS       DASVJL010000405         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_629844, whole genome shotgun sequence.
ACCESSION   DASVJL010000405 DASVJL010000000
VERSION     DASVJL010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629844"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /gene="tig"
                     /locus_tag="VF357_08565"
     CDS             complement(<1..1124)
                     /gene="tig"
                     /locus_tag="VF357_08565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX7524242.1"
                     /translation="MKTSVDAVSGVERKITVEIPAEEVDRRIEQEFTELRRMVPVRGF
                     RKGKAPMEMVKRMFRDSVEAEVSEHLVKESLTEVVKEKDLKVLSLPGVDGAKVVPGKD
                     FVFSATVEVVPEVEPVGYKGIPVARETVSVTDEDVSAAVERLRESFAQFHPVEGRGVA
                     ESDLVEFGFTAVAGGETVDRSDSTAVVLSGGLPYGEEFERRMLGVEPGTTRAFEVAYP
                     ADFRNPKYAGKTVAFEVKVGGVREKKLPEMDDAFAKQFGDVSGVDELKVKVRERLVAE
                     GEEHSRHHAEEALRNALAERNPFDVPATLVKRQTIAMMQDTFQRLASQGVDLKKMNMD
                     VDKMSERFAPNAERMVRVSLLIDAIARKENIDASYSEIDA"
     gene            complement(1171..1252)
                     /locus_tag="VF357_08570"
     tRNA            complement(1171..1252)
                     /locus_tag="VF357_08570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1217..1219),aa:Leu,seq:tag)
     gene            complement(1331..>1551)
                     /locus_tag="VF357_08575"
     CDS             complement(1331..>1551)
                     /locus_tag="VF357_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524243.1"
                     /translation="GIHIMYDTLMTFRNRIDATIFPEGKPPVRVQGTVAWFRAADAPA
                     GFYIFGMRFDQEAPSLEELRLAARKDPG"
BASE COUNT          242 a          567 c          447 g          295 t
ORIGIN      
        1 tccgcgtcga tctcggaata cgacgcatcg atgttctcct tccgcgcgat cgcgtcgatg
       61 aggaggctca cgcgcaccat ccgctccgcg ttcggcgcga accgctcgga catcttatcg
      121 acgtccatgt tcattttctt caggtccact ccctgggagg cgagccgctg aaaggtgtcc
      181 tgcatcatcg cgatcgtctg gcgcttgacc agcgtggcgg gaacgtcgaa cgggttgcgc
      241 tccgccaacg cgttgcgcag cgcctcctcc gcgtggtggc gggagtgctc ttcgccttcc
      301 gccacgagcc gctcccgcac cttcaccttg agttcgtcca cgccggacac gtcgccgaac
      361 tgcttcgcga acgcgtcgtc catctcgggg agtttctttt cgcgcacacc gcctaccttg
      421 acctcgaacg ccacggtctt gcccgcgtac ttcgggttcc ggaagtccgc ggggtaggcg
      481 acctcgaagg cgcgcgtcgt ccccggttcg acccccagca tccgtcgctc gaactcctcc
      541 ccgtacggca agccgccgga gagcacgacg gccgtcgagt cgctccgatc gaccgtttcc
      601 cctccggcca cggcggtgaa gccgaactcc accaggtccg attccgcgac gccgcgccct
      661 tccaccgggt ggaactgcgc gaacgactcc cgaagccgct cgaccgccgc cgagacgtct
      721 tcgtcggtca cgctcaccgt ttccctcgcc accgggatcc ccttgtagcc gaccggctcg
      781 acctcgggga cgacctcgac ggtggcggag aagacgaaat ccttcccggg gacgaccttc
      841 gcgccgtcga cgcccggcag ggaaaggact ttgaggtcct tttccttcac cacctcggtg
      901 agcgactcct tcaccaggtg ctccgacacc tcggcctcga ccgagtcgcg gaacatccgc
      961 ttcaccatct ccatcggagc cttccccttg cggaaccccc gcaccggcac catccggcga
     1021 agctccgtga actcctgctc gatccggcgg tccacctctt ccgccgggat ctcgaccgtg
     1081 atcttccgtt cgacgccgga aacggcgtcc acgctggttt tcatcgctct acggttcccc
     1141 tttttcgttc gatgatgtgc gacggcaacc tggtgcgaga ggagggactt gaaccctcac
     1201 ggttgcccac cggatcctaa atccggcgcg tctgccattc cgccactctc gccccgttcg
     1261 atggcgccga atcatgtagt ataaacgatt cccccgcccg cctcaacgcc gaacaaccgg
     1321 cgaacgatcc ttatccgggg tctttccggg cggcgagccg gagctcctcg agggacggcg
     1381 cctcctggtc gaatcgcatg ccgaagatgt agaagcccgc cggtgcgtcc gcggcgcgga
     1441 accaggccac ggtaccctgc acccggacag gcggtttccc ctcgggaaag atggtggcgt
     1501 cgatccggtt ccggaaggtc atgagggtgt cgtacatgat gtggatgccg t
//
LOCUS       DASVJL010000406         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1195711, whole genome shotgun sequence.
ACCESSION   DASVJL010000406 DASVJL010000000
VERSION     DASVJL010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195711"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1416)
                     /locus_tag="VF357_08580"
     CDS             complement(22..1416)
                     /locus_tag="VF357_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7524244.1"
                     /translation="MQTEPLLEKKMSFEEFGLRPEILRAVAEKNYTIPTPIQEKAIPI
                     VLEGKDLIGCAQTGTGKTAAFALPILHRLQGTPWKGAGRRPIRVLVLTPTRELASQIA
                     ESFGAYGRHTALKHAIVFGGVNQGPQSQALRRGIDILVATPGRLLDLMSQGLVQLRTV
                     ETFVLDEADRMLDMGFIHDIRRVIDQLPAKRQTLFFSATMPREIQGLADTILRDPIRV
                     AVTPVATPAEAVEQRVHYVEKSEKIKLLKHLLDGPSIKNALVFTRTKHGADAVTKQLE
                     RYSVRAEAIHGNKSQNAREKALASFKRGVTRVLVATDIAARGLDIVDLSHVVNFDLPN
                     EPESYVHRIGRTGRAGASGIALSFCSVDERPFLADIERLIHKHLPVVEDHPYRSGYGV
                     GTPTDLDPRHAHGGAALSQAPPGMPSRRGKYDASNTRPDGRPGRTRRESKWTPGFGAS
                     KPAPRHAEHRRAEW"
BASE COUNT          206 a          482 c          560 g          296 t
ORIGIN      
        1 cgcgaatctt accggtttat tttaccactc cgcgcggcga tgctcggcgt ggcggggggc
       61 cggcttgctc gccccgaacc ccggagtcca cttcgattcg cggcgcgtgc ggccggggcg
      121 gccgtcggga cgcgtgttgg aggcgtcgta cttcccccgg cgggacggca tgccgggggg
      181 ggcctgggag agcgccgccc cgccgtgcgc gtgccgcggg tcgaggtccg tcggtgtccc
      241 tacgccgtac ccggagcggt acggatggtc ctccaccacc gggaggtgtt tgtggatgag
      301 ccgttcgata tcggcgagga aggggcgctc gtcgacggag cagaacgaca gggcgatccc
      361 ggaggcgccg gcccggcccg tgcgtccgat ccggtggacg tagctttcgg gctcgttcgg
      421 gaggtcgaag ttgaccacgt gcgacaggtc gacgatgtcg aggccccggg cggcgatgtc
      481 cgtcgccacg agcactcttg tcacgccccg cttgaagctc gccagcgcct tctcgcgggc
      541 gttttgcgac ttgttgccgt ggatcgcctc ggcccggacc gagtagcgct cgagctgttt
      601 ggtgaccgcg tcggcgccgt gcttggtccg cgtgaagacc agcgcgttct tgatggaagg
      661 cccgtccagg aggtgcttga ggagcttgat tttttcggat ttctccacgt agtggacccg
      721 ctgctccacc gcctcggcgg gagtcgccac cggggttacg gccacccgga tcgggtcgcg
      781 aaggatggtg tcggcgagcc cctggatctc cctaggcatc gtcgccgaga agaagagggt
      841 ctgccgcttc gcgggcagtt ggtcgatgac gcgccggatg tcgtggatga agcccatgtc
      901 gagcatccgg tcggcctcgt cgaggacgaa cgtttcgacg gtccgcagct gcacgagccc
      961 ctgcgacatc aggtcgagga gccgtccggg cgtggcgacg aggatgtcga tcccccgccg
     1021 gagagcctgc gactgcggtc cctggttgac gccgccgaag acgatggcgt gcttgagggc
     1081 cgtgtgccgg ccgtacgcgc cgaagctctc cgcgatctgg gaggccagtt cccgcgtcgg
     1141 cgtcagcacg aggacgcgga tggggcgtcg tccggcccct ttccagggag ttccctggag
     1201 gcggtgcagg atcgggagcg cgaaggcggc cgttttcccg gtgccggtct gcgcgcagcc
     1261 gatcaggtcc ttcccctcga gcacgatggg gatcgccttc tcctggatgg gggtcgggat
     1321 ggtgtaattc ttctccgcga cggcgcggag gatctcgggg cggagcccga actcttcaaa
     1381 cgacatcttc ttttccaaca gtggttctgt ctgcatcttc tctcttctcc tgacgttttt
     1441 tttcaccaag cacaaccggt gggcgggaca gtgtgcccgt ccgatcttcc gcatggtgaa
     1501 atctcgggag aagctggatg gcccggccgg ggtgccgggc tgct
//
LOCUS       DASVJL010000407         4204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1287993, whole genome shotgun sequence.
ACCESSION   DASVJL010000407 DASVJL010000000
VERSION     DASVJL010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287993"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VF357_08585"
     CDS             <1..1263
                     /locus_tag="VF357_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX7524245.1"
                     /translation="REEGRRILLATVAGDVHDIGKNLVDIILSNNGYRVVNLGIKVSA
                     ETIIEKARELRVDAIGLSGLLVKSALVMRENLPQFAAAGLRVPVLLGGAALTGKFVAQ
                     ECVPGYPGPVIYCADAFAGLSAMRRNDEGTLSSTVFDAAGTATAMIPGPRGAAVARDN
                     PVPPPPFLGARHVDRIDPRELFPYVNEQALFRGRWGYRRGKMTAEAYEELVGGKVRPM
                     YEELKRRGVEEKILAPKVAYGWFRAFAEGDTLVVEHDGRSWPFPFPRQKNPPHLCIAD
                     YFRTRAEGGDVAGFFVATIGKEMALATQELFTSDRYHDYLMLHAFGVEVTDALAEYWH
                     EVMRRELGISGDRPAAFSGYVVQEYQGSRYGFGYPSCPDLSAHTAVFELLDPGRIGVA
                     LTESMEMVPEQTTSAIVVHHPQAKYFAV"
     gene            1289..1438
                     /locus_tag="VF357_08590"
     CDS             1289..1438
                     /locus_tag="VF357_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX7524246.1"
                     /translation="MCTNCGYVYDPAAGDPMNGIPPGTAFDDLPPGWVCPMCYAPKSS
                     FDELE"
     gene            1459..3267
                     /locus_tag="VF357_08595"
     CDS             1459..3267
                     /locus_tag="VF357_08595"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX7524247.1"
                     /translation="MKKAPLIFDGAVGTMIYDRGVFINTCYDELCLTNPKLILQIHRD
                     YVEAGAEVIETNTFGANPIRLKQYGLAEKTEAINRAGVRLAREAAGEEIFVAGSVGPC
                     TESRQRMPDAFAPEVEAAFRTQMGALAAEGVDLFLLETFSNLNELQLAARVGKDFGKP
                     VLASFVLNDRGETALGMRAETMASALSGDDNVDIVGINCGTGPSGAFDALQTILPHTG
                     KPVVVMPNAGMPREISGRVMYLTSPEYFTEYAKKYIELGVRGVGGCCGTTPAHIRMAA
                     RAVKPLRVGKRRVEVKPLLRQESLVYAVPTERKSRLAAKMCAGEKVTSVELVPPKSSD
                     MTALLDRARKCAEAGVDAINIPDGPRASARVSPMISALTILQQVGIEPILHYCCRDRN
                     LIGMQSDLLGGYAAKLANFLIITGDPPKLGEYPDVTGVFDVDSIGLVQVAANLNRGLD
                     IGGNPIDPPTGLFIGVGANPCAVDLEREIERYFRKIDAGAEFAITQPVFDPEALLRFL
                     DRVETYRRKIPVLAGVWPLISFKNAEFMNNEVPGVVVPKAILERMSRCRTREDGRKAG
                     IEIARGIVERISDRVNGFQVSAPMGHVETALAVLVK"
     gene            3331..3708
                     /gene="gcvH"
                     /locus_tag="VF357_08600"
     CDS             3331..3708
                     /gene="gcvH"
                     /locus_tag="VF357_08600"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX7524248.1"
                     /translation="MDETGLKFSEDHVWVLEMGPTARIGVSDYGQEQLGEILSAALCE
                     VGKFLEPGDTFSELESQKTVIELSSPVTGTVRAVNEAIRDDPSVINVDPYGKGWIVEV
                     EIEEPEELERLMNGEEYETFAEE"
     gene            complement(3796..>4204)
                     /locus_tag="VF357_08605"
     CDS             complement(3796..>4204)
                     /locus_tag="VF357_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524249.1"
                     /translation="AYGVTPAPDVMAAGKTKCIECHDLKKGTQTVLTVKAKCEGCHDA
                     KYGKMLLDWKQEITKQENAIAVALEEAKEYVARTKKAGKDVSKEETLVLQADANYQAV
                     SAGRGVHNNKLSREMLRAARADLDKVLAVKRKN"
BASE COUNT          707 a         1375 c         1433 g          689 t
ORIGIN      
        1 cgcgaggagg ggcgccggat cctgctggcc accgtggccg gggacgtgca cgacatcggg
       61 aagaacctgg tcgacatcat cctttcgaac aacgggtacc gggtcgtgaa cctgggaatc
      121 aaggtgtcgg cggagacgat catcgaaaag gcgcgggagc tccgggtgga cgcgatcggg
      181 ctgtcggggc tgctggtaaa atccgccctg gtgatgcggg agaacctgcc gcagttcgcc
      241 gccgcggggc ttcgcgtacc cgtcctcctc gggggggcgg ccctcaccgg gaaattcgtg
      301 gcgcaggagt gcgtcccggg gtaccccggg ccggtgatct attgcgccga cgcgttcgcg
      361 ggcctgtcgg ccatgcgtcg gaacgacgag gggacacttt cgtcgacggt cttcgacgcg
      421 gccggaaccg ccaccgccat gattcccggc ccccgagggg cggccgtcgc ccgggacaac
      481 ccggtgccgc cgccaccgtt cctcggtgcc cgccacgtcg accggatcga cccgcgggaa
      541 ctgttcccct acgtcaacga acaggccctt ttccgcgggc ggtgggggta ccggcgggga
      601 aagatgaccg ccgaggcgta cgaggaactg gttgggggga aggtccggcc gatgtatgaa
      661 gagctgaagc ggcgcggcgt ggaggagaag attctcgcgc cgaaggtcgc ctacgggtgg
      721 ttccgcgcct tcgcggaggg ggacacgctc gtagtggagc acgacgggcg ctcgtggccc
      781 tttcccttcc cccggcagaa gaacccgcct cacctgtgca tcgcggacta tttccggacg
      841 cgggcggagg ggggagacgt cgccggcttc ttcgtggcga ccatcgggaa ggagatggcc
      901 ctggcgacac aggagctctt cacgtccgac cggtaccacg actacctgat gctccacgcc
      961 ttcggagtcg aggtcaccga cgccctggcc gagtactggc acgaggtcat gcgccgcgag
     1021 ctcggaatct ccggggaccg gccggccgcc ttctccggtt acgtcgtcca ggagtaccag
     1081 gggtcccgct acggcttcgg gtacccttcc tgcccggacc tctccgcgca cacggcggtg
     1141 ttcgaactgc tcgacccggg ccggatcgga gtggcgctca ccgagtcgat ggagatggtg
     1201 cccgagcaga ccacctcggc gatcgtcgtc caccaccccc aggcgaaata tttcgcagtg
     1261 taggaggagg cgcgccatca agaaatacat ctgcacgaac tgcggctacg tctatgaccc
     1321 ggcggccggg gatccgatga acgggatccc gccggggacc gcgttcgacg acctgccccc
     1381 ggggtgggtc tgcccgatgt gctacgcccc caaatcctcg ttcgacgaac tggagtagcc
     1441 ctggacctcc ttgcgcgcat gaagaaggcg ccgctcatct tcgacggcgc cgtgggaacg
     1501 atgatctacg accgcggtgt attcatcaac acctgctacg acgagctctg cctcacgaac
     1561 ccgaaactca tcctccagat ccaccgcgac tacgtggagg ccggggccga ggtgatcgag
     1621 acgaacacct tcggcgccaa cccgatccgg ctcaagcagt acggcctcgc ggagaagacg
     1681 gaggcgatca atcgcgccgg ggtccggctg gcgcgggagg cggcggggga ggaaatcttc
     1741 gtcgcggggt cggtgggccc ttgcaccgag tcccggcagc ggatgcccga cgcgttcgcc
     1801 cccgaggtcg aggcggcgtt ccggacccag atgggggccc tcgcggcgga gggggtggac
     1861 ctgttcctcc tcgagacctt ctccaacctg aacgagctcc agctcgccgc gcgggtgggg
     1921 aaggacttcg gcaaaccggt cctcgcctcc ttcgtcctga acgatcgggg agagacggcc
     1981 ctggggatgc gggcggaaac gatggcgtcg gccctttccg gcgacgacaa cgtggacatc
     2041 gtcgggatca actgcggcac gggcccttcc ggtgccttcg acgcgctcca gacgatcctc
     2101 cctcacaccg gcaaaccggt ggttgtcatg ccgaacgcgg ggatgccccg ggagatcagc
     2161 gggcgcgtga tgtacctgac gagcccggaa tacttcaccg agtacgccaa gaagtacatc
     2221 gagctggggg tccggggcgt gggggggtgc tgcgggacga cgcccgcgca catccggatg
     2281 gcggcgcgcg cggtgaaacc gctgcgcgtc gggaagcggc gcgtggaggt gaaaccgctc
     2341 ctccggcagg agtcgctggt ctatgcggtc ccgaccgaac ggaagtcccg cctcgcggcg
     2401 aagatgtgcg cgggggagaa ggtgacgtcg gtggagctcg tcccgccgaa atcgagcgac
     2461 atgaccgcgc tgctcgacag ggcgcggaag tgcgcggagg ccggcgtcga cgcgatcaac
     2521 atccccgacg gcccccgggc gagcgcgcgg gtctccccga tgatttccgc cctcaccatc
     2581 ctgcagcagg tcgggatcga gccgatcctg cactactgct gccgcgaccg gaacctgatc
     2641 gggatgcagt ccgacctgct ggggggatat gccgcgaagc tcgcaaactt cctcatcatc
     2701 accggggacc ccccgaagct gggagagtac cccgacgtga ccggcgtctt cgacgtcgac
     2761 tcgatcgggc tggtgcaggt ggcggccaac ctgaaccgcg ggctggacat cggcggcaac
     2821 ccgatcgatc cgcccacggg cctgttcatc ggggtcggcg ccaatccgtg cgcggtggac
     2881 ctggagcgcg agatcgagcg gtacttccgg aagatcgacg caggggcgga attcgccatc
     2941 acgcagccgg tgttcgatcc cgaggcgttg ctgcggttcc tcgaccgcgt ggaaacgtac
     3001 cgccggaaga ttcccgtgct cgcgggcgtg tggcccctca tcagcttcaa gaacgccgag
     3061 ttcatgaaca acgaggtccc cggcgtggtc gtgccgaaag cgattctcga acggatgtcc
     3121 cggtgcagga ccagggagga cggccggaag gcggggatcg agatcgcccg cgggatcgtc
     3181 gagaggatct cggaccgcgt gaacggcttc caggtgagcg ccccgatggg gcacgtcgag
     3241 acggcgctgg cggttctcgt aaaataatcc tgaaactttt ctccccttct gttgacaaat
     3301 tcctcccgcg tcgaagtaca ctggcagcct atggacgaga ccggactgaa attctccgaa
     3361 gatcacgtct gggtcctcga gatgggaccc accgcccgca tcggcgtttc cgattacggg
     3421 caggagcagc tgggggagat tctctccgcg gccctgtgcg aggtcgggaa gttcctggag
     3481 ccgggagaca cgttcagcga gctggaatcc cagaagaccg tgatcgaact ctcctccccc
     3541 gtcacgggga cggtccgggc cgtcaacgaa gcgatccggg acgacccctc cgtgatcaac
     3601 gtcgacccgt acggcaaggg gtggatcgtc gaggtcgaga tcgaggagcc cgaggagctc
     3661 gagcggctga tgaacggcga agagtacgaa acgttcgcgg aagagtagcc cgctcctccg
     3721 gcaccctgac aagcaaacgc gaagcgcccc gggagatcga tctcccgggg cgcttcgcgt
     3781 ttccggcaag caagatcagt tcttccgttt gaccgccagc accttgtcga ggtcggcccg
     3841 cgccgcccgg agcatctccc tgctgagctt gttgttgtgg actccgcggc cggcggagac
     3901 cgcttggtag ttcgcgtccg cctggaggac cagcgtctcc tccttcgaga cgtcctttcc
     3961 cgccttcttc gtgcgggcga cgtactcctt cgcctcctcg agcgccacgg cgatggcgtt
     4021 ctcctgcttc gtgatctcct gcttccagtc gaggagcatc tttccgtact tcgcgtcgtg
     4081 gcacccctcg cacttcgcct tgacggtcag caccgtctgc gtccccttct tcaggtcgtg
     4141 gcactcgatg catttcgttt taccggccgc catcacgtcc ggcgccgggg tcacgccgta
     4201 cgcc
//
LOCUS       DASVJL010000408         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1662673, whole genome shotgun sequence.
ACCESSION   DASVJL010000408 DASVJL010000000
VERSION     DASVJL010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662673"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..427
                     /locus_tag="VF357_08610"
     CDS             29..427
                     /locus_tag="VF357_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7524250.1"
                     /translation="MKLKTSEDHQQIGELSRSLGVTTRTLRLYEQMGLIDPPQRTEGG
                     IRYYTKDDNRRIKFVLKLKELGLSLQQMQELAEIYHETKMPDKIMPRLIELLDSHTDA
                     IHRRIGKLSSLARDISEYRKRIVDFYAILK"
     gene            complement(477..1031)
                     /locus_tag="VF357_08615"
     CDS             complement(477..1031)
                     /locus_tag="VF357_08615"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEX7524251.1"
                     /translation="MRKLAVALSFLLLSSQAFALDVAGVNVAPTVSVHQKTLTLNGAG
                     IRKKLFFKVYVGSLYTERKVTTPTQLLADPGEKLIRMSFVYSKVEKEKIVEAFAEGLA
                     NNSPGVARSAEAKAFLSWFTADFVAGDTVDISLSPDGTVAASHNGKDLGTVRSPELVQ
                     GVLLIWFGEKPADGSLKKGMLGNG"
     gene            complement(1301..>2416)
                     /locus_tag="VF357_08620"
     CDS             complement(1301..>2416)
                     /locus_tag="VF357_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7524252.1"
                     /translation="KLGIRGSSTTSLILEDAKVPVENVLGEIGKGHKIAFNVLNVGRL
                     KLGSCVTGAGKFAFADGVRYANLRKQFGVTIGTFGAIREKIADTASALFASEALVYRL
                     AGMIDDRLAAVPKGIPDYYDAYQQGIEDYSIECAIAKVFCSDVLAQVVDEVVQIFGGY
                     GYTQEYPAERYYRDERINRIFEGTNEINRMLIPGTLLRRAMKGEIPLQDEVMKAVGRL
                     SATAVEAPSGPFGAEKALLRNLKDAFLVLAGAAVQRFGGKVKDEQETLIALADVAIGV
                     FAIESAVLRAEKIASSGNASRAELAAAAVKVFAFPASEAAVSAARRAAFYVGEGNTLQ
                     MLLSGIRRSTRYDASGLLEAKHKLAAAALELERYPFG"
BASE COUNT          427 a          811 c          751 g          427 t
ORIGIN      
        1 ttaacattga cgttgaggta aagtacggat gaagctgaaa accagcgaag accaccagca
       61 gatcggggag ctttcccgtt cgctcggagt aacgacccgg acgctgcggc tctacgagca
      121 gatggggctg atcgacccgc cgcaacggac cgaggggggg atccggtatt acacgaagga
      181 cgacaaccgg aggatcaagt tcgtcctgaa attgaaggaa ctgggtctat cgctccagca
      241 gatgcaggag ctggcggaaa tttaccacga gacgaagatg ccggacaaga tcatgccgcg
      301 cctgatcgag ctgctcgact cccacacgga cgcgatccac cggaggatcg ggaagctgtc
      361 gtccctggcg cgggacatct cggagtaccg gaagcggatc gtcgacttct acgccatcct
      421 gaagtagcac aaagccatgg gctccgcgct tgttacgtag ccgtagttcg gccgtctcat
      481 ccgttcccga gcatcccctt cttcaagctc ccgtcggccg gcttctcccc gaaccagatg
      541 aggagcaccc cctggacgag ctccggggac cggaccgtcc cgaggtcctt cccgttgtgc
      601 gacgcggcca ccgtgccgtc gggggaaagg gagatgtcga ccgtgtcgcc cgcgacgaag
      661 tcggccgtga accaggagag gaacgccttc gcctccgcgg agcgcgcgac accgggggag
      721 ttgttcgcga gaccctcggc gaacgcctcg acgatcttct ccttctccac cttgctgtac
      781 acgaagctca tccggataag cttctcgccg gggtcggcga ggagctgcgt gggggtcgtc
      841 accttccgct ccgtatagag cgacccgacg tacaccttga agaagagttt cttgcggatc
      901 cccgcgccgt tgagggtgag cgtcttctgg tggacggaaa ccgtcggggc gacgttcacc
      961 ccggcgacgt ccagcgcaaa cgcttgtgac gacagcagca ggaacgacag cgcgacggcg
     1021 agcttcctca tccggtaccc cccgggttcg aagtattcca gatcatacta tgcttggcta
     1081 gttgggggag gcgctttgat ctccgcgtga tacccccttc ctcgctacgt tccggccgcc
     1141 ctccctggct gccttcactg gccgctcctg cgcctccatg cgcccgcggc acttggccat
     1201 ccatggccat cgacgatggt agcagagtgg aagcgccccc gcgagcccgg agccctatcc
     1261 tgcctcgctc acctgtcacc gtatttatga actgaaacac ttagccgaac gggtagcgct
     1321 ccaattcaag ggccgcggcg gcgagcttgt gcttcgcttc gaggaggccg gaggcgtcgt
     1381 accgggtcga acgccggatc ccggagagga gcatctgcag cgtgttcccc tcgccgacgt
     1441 agaacgccgc gcgccgcgcc gcgctgaccg ccgcctcgga cgccgggaac gcgaaaacct
     1501 tgacggccgc cgcggcgagc tccgcgcgag aggcgttccc ggaggaggcg atcttctccg
     1561 cccgcagcac cgcgctttcg atcgcgaaga cgccgatcgc gacgtccgcg agtgcgatca
     1621 gggtttcctg ctcgtccttc accttcccgc cgaaccgctg cacggccgcc cccgcaagca
     1681 cgaggaaggc gtccttcagg ttgcgcagca gcgccttctc ggcgccgaac ggaccggaag
     1741 gcgcttccac cgcggtggcg gagaggcgtc ccaccgcctt catcacctcg tcctgcaggg
     1801 ggatctctcc cttcatcgcc cggcgcagaa gcgtcccagg gatgagcatc cggttgatct
     1861 cgttcgtccc ctcgaagatc cggttgatcc gctcgtcgcg gtaatatcgc tccgcgggat
     1921 actcctgcgt gtagccgtac ccgccgaaga tctggacgac ctcgtccacc acctgcgcga
     1981 ggacgtcgct gcagaagacc ttcgcgatgg cgcattcgat cgagtaatcc tcgatcccct
     2041 gctggtacgc gtcgtagtag tcggggatcc ccttcgggac ggcggccagg cggtcgtcga
     2101 tcatcccggc caggcgatac accagggcct ctgaggcgaa cagcgccgac gcggtgtcgg
     2161 cgatcttctc ccggatggcg ccgaacgttc cgatggtgac gccgaactgc ttgcgcaggt
     2221 tcgcgtaccg caccccgtcc gcgaaggcga attttccggc gccggtgacg caggagccga
     2281 gcttcaggcg cccgacgttc agcacgttga aggcgatctt gtgccccttg ccgatctcgc
     2341 cgagcacgtt ttccacgggg accttcgcgt cctcgagaat cagcgatgtc gtcgaggaac
     2401 cgcggatccc gagctt
//
LOCUS       DASVJL010000409         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1570492, whole genome shotgun sequence.
ACCESSION   DASVJL010000409 DASVJL010000000
VERSION     DASVJL010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570492"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VF357_08625"
     CDS             <1..410
                     /locus_tag="VF357_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX7524253.1"
                     /translation="AAARSGAVAAMIDVSDGIHTDLSHLLESDGLGAVLAEESFPVSR
                     SFRAASAALGVDPLDAFLGGGEDYELLMAVHPARRASFLRAARSFPSGATLVGAVTKA
                     AGIRVRRADGSWLEGAALPSGFGHFVPPKRRTR"
     gene            418..1674
                     /locus_tag="VF357_08630"
     CDS             418..1674
                     /locus_tag="VF357_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX7524254.1"
                     /translation="MKAILLPFLFLASAFFSATETALFALRRVDLLKWKEEGNRRAER
                     IAQMLSKPNRLIATIFLGNEIANVAISSVIAALLLPRLPAGAGEGLALLVGTLGILLI
                     GDVTPKCLVWSRARGFSLVAAAPLHAFSRVVAPARFVLEKIAGGILFLLGERGGPQTR
                     EMISEAEFRALVDAGEETGTLDSEEKELIHNIFEMTDRRAGEIMTPLPDVFMIPRDLP
                     YEELVARYRLYRRSRIPVYEGERHNVVGVLHFKQLLRPMAEGGKPPVWQDLVRPPYVV
                     PGVQRLPPLLREFQRRKVHIAVVVDEYGETSGIVTLEDVLEELFGEIREERDREEREI
                     VRRPDGSSLVLGKTQIRHFNEAFGTGIPDLEFDTVAGFLLHEFGRMPAPGEKTSQEGI
                     SFTVERMKGLRIVEIGVRPALPGKEE"
BASE COUNT          245 a          600 c          635 g          311 t
ORIGIN      
        1 aggccgccgc gcgctccggc gcggtcgcgg cgatgatcga cgtgagcgac gggatccaca
       61 cggacctctc gcacctcctc gagagcgacg gcctcggcgc cgtcctcgcc gaggagtcgt
      121 ttcccgtctc ccggtcgttc cgcgcggcgt ccgccgccct cggggtcgac ccgctggacg
      181 cgttcctggg gggcggggag gattacgaac tgctgatggc cgtccatccc gcgcggcgcg
      241 cgagctttct gcgcgcagcg cgctctttcc cttcgggcgc gaccctggtc ggcgcggtga
      301 cgaaggccgc ggggatccgc gtccggcgcg cggacggttc gtggctcgag ggcgccgcgc
      361 tcccttccgg attcggtcac ttcgtccccc cgaagcggcg tacccgctga cgccgtcgtg
      421 aaagcgatcc tcctcccctt tctttttctc gcctccgcgt tcttttccgc gacggagacg
      481 gcgctgttcg ccctgcgccg ggtggatctt ctgaagtgga aggaggaggg gaaccgccga
      541 gccgagcgga tcgcgcagat gctctcgaag cccaaccggc tgatcgcgac gatcttcctc
      601 gggaacgaga tcgccaacgt tgcgatctcc tccgtcatcg cggccctgct cctgccgcgt
      661 cttccggccg gggctggcga gggcctcgcc cttctcgtgg gtacgctggg gatcctgctg
      721 atcggggacg tgacgccgaa gtgcctcgtg tggtcgcggg cccgcggttt ttcgctggtg
      781 gcggccgcgc cgctccacgc gttctcgcgc gtggtcgctc ccgcgcgatt cgtgctcgag
      841 aagatcgccg gcgggatcct cttcctgctg ggggagcggg gcgggccgca gacccgcgag
      901 atgatctccg aggcggagtt ccgcgcgctg gtcgacgccg gagaggagac cggcactctc
      961 gactcggagg agaaggagct catccacaat atcttcgaaa tgaccgatcg gcgcgccggg
     1021 gagatcatga cgcccctgcc cgacgtcttc atgatcccga gggatctgcc gtacgaggag
     1081 ctggtcgccc ggtaccgcct ctaccgccgg tcccggattc cggtctacga gggcgagcgg
     1141 cacaacgtcg tcggcgtcct ccatttcaag caactgctcc gcccgatggc cgaggggggg
     1201 aagccgccgg tgtggcagga tctcgtccgg cctccctacg tcgtccccgg ggtgcagaga
     1261 ctcccgccgc tgctgcggga gttccagagg cggaaggtgc acatcgccgt cgtcgtggac
     1321 gagtacgggg agacgtccgg gatcgtgacg ctggaggacg tcctcgagga gctgttcggg
     1381 gagatccggg aggagcgcga ccgggaagag agggagatcg tgcggcgccc cgacggttcc
     1441 tccctcgtcc tcgggaagac gcagatccgc catttcaacg aggcgttcgg gaccgggatc
     1501 ccggatctgg aattcgacac ggtcgccggt ttccttctcc acgagttcgg gaggatgccg
     1561 gcccccgggg aaaagacctc gcaggagggc atctccttca ccgtggagcg gatgaagggg
     1621 ctgcggatcg tcgagattgg cgtgcgcccc gcactgccgg ggaaggagga atagcatggg
     1681 aatcgtcctc ctcgtcgcct tctgcctcct gatggaggcg ctcttcaccg gtgccgagat
     1741 ggtgctggtc tcggcggacc ggcacaagct caccgagcgg tcccgccagg g
//
LOCUS       DASVJL010000410         3242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1477477, whole genome shotgun sequence.
ACCESSION   DASVJL010000410 DASVJL010000000
VERSION     DASVJL010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477477"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VF357_08635"
     CDS             <1..562
                     /locus_tag="VF357_08635"
                     /inference="COORDINATES: protein motif:HMM:NF023958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524255.1"
                     /translation="VTTEARQACDRLLRAGVPVNNQTVLLKGVNDHAEIIRKLNTELL
                     RAKVRPYYLFQCDMVRGLDHFRTRLSKGIQIMEALRGLTSGLAIPSFVVDVPGGGGKI
                     PLMPNYILSMGEDRTVLRNYEGIIVSYEEARDDGRPSARVDSALPPPRNDVYRLLTGE
                     VRALIPAGNERMARRKRRCESGSPST"
     gene            535..1527
                     /locus_tag="VF357_08640"
     CDS             535..1527
                     /locus_tag="VF357_08640"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524256.1"
                     /translation="MRVGLAFNVKPADTPDHLPEDAFEEYDSEATVAHIENALSRLGH
                     DVRRLPAGRGFVDAVRDAAPDIVFNIAEGEGGRCREAHVPAVLEMLGVPFVGSDPLTL
                     CVTLDKPVAKRLVEHDGFPTPPFRTFRSAEELDSLPFPFPVIVKPAFEGSSKGVRIAS
                     RATSLDQAREMVRFVTQTYRQDAMVEAFVPGAEVTVGVLGNDPPRIAGMMEIVPKTVS
                     PAEFVYSLEVKRDWENRVAYRVPPALPAAVLSEIGRCALGIYRTLGCRDFSRIDFRLD
                     PAGSPQFIECNPLPGLSPGYGDLTIMADRMGIPYLSLVGEILSHALRRLGIPST"
     gene            1524..2552
                     /locus_tag="VF357_08645"
     CDS             1524..2552
                     /locus_tag="VF357_08645"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524257.1"
                     /translation="MRSAAANGARIAVLYTSVFEELEDVKEEIREDMDLALNAKSVEE
                     ALRSGGHDAWTHPFGIDPGELISRLRSSRAEAVFNLSECPNLSPEKELHACALLELLL
                     LPYTGNGPLALGICNSKALSKQLMIANGIPTPRFRLYNRNPRGEPGLSYPLVVKPANE
                     DGSAGITEDSVVADLAGLRRQVKWLREGFLQNSLVEEFVEGREFNVGVLGNGTAADPH
                     RSLPPAELVYRNPRWRLCTYESKWNSAHPSYAEIAPVCPAKAPPETAARLSEITLACA
                     RIFGLTGYARIDFRMNAGGELFVLEVNPNPDISPDAGMARAARAAGLPYPDLILEILR
                     LGLALGAR"
     gene            2549..3028
                     /locus_tag="VF357_08650"
     CDS             2549..3028
                     /locus_tag="VF357_08650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7524258.1"
                     /translation="MTCIRRIEPGDREAIRALIAGTDAFKPFEVDVAMELVDVALTQK
                     TQDDYHPYVLVEDDGTVVAYACFGKNAMTTGTFDLYWIATRTDRMGKGYGRAILSFVE
                     EEVRRRGGYLLVIETSSQESYGSTRLFYDKVGCTLAAQLPDYYAKGDDKLIYLKRVR"
     gene            complement(3048..>3242)
                     /locus_tag="VF357_08655"
     CDS             complement(3048..>3242)
                     /locus_tag="VF357_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX7524259.1"
                     /translation="VVRAPGPSGELWVIDYKTGERDRALEEAYFLQLREYRDILSEAW
                     RVPVRAFLWYVESGASFEVK"
BASE COUNT          532 a         1111 c         1077 g          522 t
ORIGIN      
        1 ggtcaccacc gaggcgcgcc aggcgtgcga ccggctcctt cgcgcgggcg tgcccgtgaa
       61 caaccagacc gtcctgctca agggcgtcaa cgaccacgcc gagatcatcc gcaagctcaa
      121 cacggaactg ctgagggcga aggtccgccc gtactatctt ttccagtgcg acatggttcg
      181 ggggctggac cacttccgca cccgcctgtc gaaagggatc cagatcatgg aggcgctgcg
      241 gggcctcacc tcggggctcg cgatcccttc cttcgtggtg gacgtccccg ggggcggggg
      301 caagatcccg ctgatgccga actacatcct ctcgatgggc gaggaccgga cggttctgcg
      361 gaactacgag gggatcatcg tgagctacga ggaggctcgg gacgacgggc gtccctccgc
      421 gcgggtcgat tcggcgctcc ctcccccgcg caacgacgtc taccggctgc tgaccggcga
      481 ggtgcgcgcg ctcatccccg ccgggaacga gcggatggcg aggaggaaac gccggtgcga
      541 gtcgggctcg ccttcaacgt aaaaccggcc gacacgcccg atcatctgcc cgaggacgcc
      601 ttcgaggagt acgactccga ggcgacggtg gcccacatcg agaacgccct ctcccgcctg
      661 ggccacgacg ttcgccgtct tcccgccggg cgcgggttcg tcgacgccgt gcgggacgcc
      721 gcgcccgaca tcgtcttcaa catcgcggaa ggagaaggcg ggcggtgccg cgaggcgcac
      781 gtcccggccg tcctcgagat gctcggcgtc cctttcgtcg gctccgatcc gctgacccta
      841 tgcgtcaccc tcgacaagcc ggtggccaag cgcctcgtgg aacacgacgg cttccccact
      901 ccgccgttcc ggaccttccg atccgccgag gagctcgatt ccctgccgtt tcccttcccc
      961 gtcatcgtga aacccgcctt cgaaggatcc agtaaggggg tccgtatcgc ttcgcgcgcc
     1021 acgtccctcg accaggcgag ggagatggtg cggttcgtca cgcagacgta ccgccaggat
     1081 gcgatggtcg aagcgttcgt gccgggggcg gaggtcacgg tgggagtgct ggggaacgat
     1141 ccgccccgga tcgccgggat gatggagatc gtcccgaaga cggtctctcc ggcggagttc
     1201 gtctactccc tggaagtgaa gagggactgg gagaaccgcg tcgcgtatcg cgtgccaccc
     1261 gcgctgccgg ccgcggtcct ctcggagatc gggcggtgcg ccctggggat ctaccggacc
     1321 ctcggatgcc gcgacttttc ccggatcgac ttcaggcttg atcccgcggg aagcccccag
     1381 ttcatcgaat gcaacccgct ccctggcctg tctccggggt acggcgacct gacgatcatg
     1441 gcggaccgga tggggatccc ttatctgtcc ctcgtcgggg agatcctctc ccacgccctt
     1501 cgtcgcctcg gtatcccttc cacgtgaggt ccgccgccgc aaacggggcg cgcatcgccg
     1561 tcctctacac ctccgttttc gaagagctcg aggatgtgaa ggaggagatc cgggaggaca
     1621 tggacctcgc gctcaacgcg aaatcggtgg aggaagcgct ccgatcgggc ggacacgacg
     1681 cctggacgca tcccttcgga atcgacccgg gggagctgat ctcgcggctg cgctcctcgc
     1741 gggccgaggc ggtcttcaat ttgagcgagt gtccgaatct ctccccggag aaggagctgc
     1801 acgcctgcgc gctcctcgag ctgctcctcc tcccgtatac agggaacggg ccgctcgcgc
     1861 tggggatctg caacagcaag gcgctctcca agcagttgat gatcgccaac gggatcccca
     1921 ccccgcgctt ccggctctac aaccgcaatc cgcgcgggga gccgggactg tcgtacccgc
     1981 tggtcgtgaa accggcgaac gaggacggct cggccgggat caccgaagac agcgtcgtcg
     2041 ccgaccttgc cggtttgcgc cgacaggtga aatggctgag ggaggggttc ctccagaatt
     2101 ccctcgtcga ggagttcgtg gaggggcggg agttcaacgt cggcgtgctc gggaacggca
     2161 cggcggccga tccgcaccgg tcgctccccc ccgcggagct cgtgtaccgg aacccgcgct
     2221 ggcggctctg cacctacgaa tcgaagtgga attcggcgca cccctcctac gcggagatcg
     2281 ccccggtctg ccccgccaag gctccgccgg aaaccgccgc gcggctttcg gagatcacgc
     2341 tggcctgcgc ccggatcttc gggctcacgg gctacgcccg tatcgacttc cggatgaacg
     2401 cgggtgggga gctgttcgtc ctcgaggtga acccgaaccc cgacatctcg cccgacgccg
     2461 ggatggcgcg cgcggcgcgc gcggcggggc tcccttaccc ggacctcatc ctcgagatcc
     2521 tgcggctggg cctggccctg ggggcgcgat gacgtgcatc cgccgcatcg aaccggggga
     2581 ccgcgaggcg atccgggcgc tcatcgctgg gaccgacgcg ttcaagccgt tcgaggtgga
     2641 cgtggcgatg gagctggtcg acgtcgccct cacgcagaaa acgcaggacg actaccatcc
     2701 gtacgtcctg gtggaggacg acggcacggt ggtcgcctac gcctgcttcg ggaagaacgc
     2761 gatgacgacg gggaccttcg acctgtactg gatcgcgacg cggacggacc ggatgggaaa
     2821 agggtacgga cgcgccatcc tgtcgttcgt cgaggaggag gtccgacgtc gcggggggta
     2881 cctcctggtg atcgagacgt cgtcgcagga gagctacggc tccacgcggc tattctacga
     2941 caaggtcggc tgcacccttg ccgcgcagct tcccgactat tacgcgaagg gcgacgacaa
     3001 gctcatctac ctgaagcgcg tccgctaaac gctggtcgcc aaccccttca cttcacttcg
     3061 aacgacgcgc cggattccac gtaccagaga aacgcgcgga cggggacgcg ccacgcctcg
     3121 gagaggatgt cgcggtactc ccgcagctgg aggaagtacg cctcctcgag cgcccggtcg
     3181 cgctccccgg tcttgtaatc gatgacccat agctcccccg acggtcccgg ggcgcgaacg
     3241 ac
//
LOCUS       DASVJL010000411         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1288711, whole genome shotgun sequence.
ACCESSION   DASVJL010000411 DASVJL010000000
VERSION     DASVJL010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288711"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VF357_08660"
     CDS             <1..579
                     /locus_tag="VF357_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7524260.1"
                     /translation="GLGEAEEAKLARGISSGIRSLDNILTNMLLFAREQKPSLTRVDM
                     AGIVDEALFLLEPMIGSRGVRIEKCVAGAPCLDGDPELLKQVLLNVLLNAIHATPAEG
                     RIRVSAREEDDAAVIEVSDEGEGIREEDLERIFDPFFSTRVKGTGLGLAITSRIMEAH
                     GGFIRVQSEVGRGSVFALHFPPAGTLSDGAVM"
     gene            576..1850
                     /locus_tag="VF357_08665"
     CDS             576..1850
                     /locus_tag="VF357_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7524261.1"
                     /translation="MKPILVVDDDPQMRSALKEAIQRIGYGADVCENGQEAIGRISRT
                     GYSLVVTDMKMPGMDGLSLLREIRRRCATQPVIVITGYATVENAVETMREGATDYLLK
                     PFSFDALKKVVLSVMARAVDEREFLTDDPGMRKILALARNIAASDITVLAQGESGTGK
                     ELLARYIHKMGRRAGKPFVAVNCAAIPENLIESELFGHEKGAFTGAAERKKGKIELAD
                     GGTILLDEIGEMPLMLQAKLLRVLQEREVDRVGGKEPVPVDVRVIATTNRDLQKECRE
                     GRFREDLYYRLNVFPIKLPPLRERPADIPLLAAHFAEKFSAAEEKTIRGFTPDGIALL
                     KNRPWRGNIRELENVIRRAVLLCLGDAIGIDALLFDEPVEVTGYNGKIREMEKELILR
                     TLKEVDGNKSRAAVLLGVTVRTIRNKLSEYGN"
     gene            1893..2258
                     /gene="flgB"
                     /locus_tag="VF357_08670"
     CDS             1893..2258
                     /gene="flgB"
                     /locus_tag="VF357_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEX7524262.1"
                     /translation="MSDIFNTLERQIHAANVRHGVIASNIANVDTPGFKARDVKFDEA
                     LETATIELRKTSPSHMGQAEDASGGKVAVDSRPSWGDGNNVELDVEVAKMTENAVFFQ
                     TAVTLLSTNIRMYKSALKR"
     gene            2261..2674
                     /gene="flgC"
                     /locus_tag="VF357_08675"
     CDS             2261..2674
                     /gene="flgC"
                     /locus_tag="VF357_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEX7524263.1"
                     /translation="MNSFDVLKISGSALEAQRRRMNVIASNLANANSTRTEEGGPYKR
                     QDVVFTSTTVESNPLELKGVKVADVVPDGKPPKSVYDPGHPDADKDGFVAMPNINTME
                     EMVNMMMAFRAYEASVATFNTAKTMLLKTLELGRV"
     gene            2674..2964
                     /gene="fliE"
                     /locus_tag="VF357_08680"
     CDS             2674..2964
                     /gene="fliE"
                     /locus_tag="VF357_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX7524264.1"
                     /translation="MTDMRIAGIGGAGILTPVKNPDKIAGGGFDNILKDAVGKISQVQ
                     KDAETAVRELSSGGDVTSAVLSMEKADMTFQLMVEVRNRLLTAYEEIMRMAI"
     gene            2980..>3544
                     /gene="fliF"
                     /locus_tag="VF357_08685"
     CDS             2980..>3544
                     /gene="fliF"
                     /locus_tag="VF357_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX7524265.1"
                     /translation="MPTSMPNVIDDVMAWPVRKKATGVAVIVASVALLILSFSWSQKE
                     EYHVLYSNLSESDAGQVVQKLKEMKVPYRADAGGIFVPADRVYDARLQLASQGLPQGG
                     GVGFELFDKTSFGTTDFVQKLNYKRALQGELARTVMAIGAVEQCRVHLAIPEKSLFAR
                     EGEHERPTASVLVKLRQGKTLSSAQVDG"
BASE COUNT          741 a         1039 c         1181 g          583 t
ORIGIN      
        1 gggctcggcg aggcggagga ggcgaaactc gcccggggaa tatcgagcgg catccggagc
       61 ctcgacaaca tcctcacgaa catgctcctt ttcgcgaggg agcagaagcc gtccctgacc
      121 cgcgtcgaca tggccgggat cgtcgacgag gccctcttcc tgctcgaacc gatgatcggc
      181 tcgcggggag tgcgcatcga gaaatgcgtc gcaggcgcgc cgtgcctcga cggtgacccg
      241 gagctcctca agcaggttct gctgaacgtc ctcctcaacg cgatccacgc gacgcctgcg
      301 gagggccgaa tccgtgtgtc cgcccgggag gaggacgatg ccgcggtcat cgaggtctcc
      361 gacgagggcg aggggattcg cgaagaggac ctggaacgga tcttcgaccc gttcttcagc
      421 acgagagtga aggggaccgg gcttgggctc gcgatcacct cgaggatcat ggaggcgcac
      481 ggcggattca tccgggtgca gagcgaggtg ggcagaggga gcgtattcgc gcttcacttc
      541 ccccccgccg ggacgttgag cgacggggcg gtaatgtgaa gccgatcctc gtagtggacg
      601 acgaccccca gatgcggtcg gccctgaaag aggcgatcca gcggatcggc tacggcgccg
      661 acgtgtgcga gaacggccag gaggccatcg gccggatctc ccgcaccggg tactcgctgg
      721 tcgtgaccga tatgaagatg ccggggatgg atggcctgtc cctcctccgg gagatccgcc
      781 gccgctgcgc gacccagccc gtcatcgtca tcacgggcta cgcgaccgtg gagaacgccg
      841 tcgagacgat gcgggagggg gcgacggatt atctcctgaa gccgttctcc ttcgatgcac
      901 tcaagaaggt cgtcctgtcc gtgatggccc gggcggtgga cgagagggag ttcctgacgg
      961 acgacccggg gatgcggaag atcctcgcgc tcgcccgcaa catcgcggcg agtgacatca
     1021 cggtcctggc tcagggggag agcggtacgg ggaaagagct cctcgcgcgc tacatccaca
     1081 agatgggccg gcgggccggg aagccgttcg ttgccgtcaa ctgcgccgcg attcccgaga
     1141 acctgattga gtccgagctg ttcgggcatg agaagggggc gttcaccggc gccgcggaga
     1201 ggaagaaggg gaaaatcgag ctcgcggacg gggggacgat cctgctcgac gagatcggcg
     1261 agatgccgct catgctccag gcgaagcttc tccgtgttct ccaggagagg gaggtcgacc
     1321 gggtcggagg caaggagccc gtgccggtcg atgtccgcgt cattgccacg acgaaccggg
     1381 atctccagaa ggagtgccgg gaaggtcgct tccgcgaaga tctctattac cggctcaatg
     1441 tgttcccgat caagctgccc cccttgaggg agcgcccggc ggacattccg ttactggcgg
     1501 ctcacttcgc ggaaaaattt tccgcggccg aggagaagac aatccggggg ttcacgccgg
     1561 atgggattgc gctgctcaag aacagaccct ggcgcgggaa catccgggag ctcgaaaacg
     1621 tcatccggcg ggccgtgctt ctctgccttg gcgacgcgat cggcatcgac gcccttctgt
     1681 tcgacgaacc ggtggaagtt accggataca atgggaaaat acgggagatg gaaaaggagc
     1741 tgatccttcg aaccctcaag gaagtcgacg gcaacaagag ccgggccgcg gtgctcctcg
     1801 gagtgaccgt ccggaccatc cggaacaaat tgagcgagta tggaaactga gcgccggatt
     1861 ctggcacgtt tgttgaagag agtggaggtg gaatgagcga tatcttcaat accttggagc
     1921 gtcagatcca cgcggcgaac gtgcgccacg gagtgatcgc ctccaacatc gccaacgtcg
     1981 acacacccgg cttcaaggcg agggacgtca agttcgacga ggcgctcgag acggcgacca
     2041 tcgagttgcg caagacgagc ccttcccaca tgggccaggc cgaggatgca tccggcggga
     2101 aggttgctgt ggattcgcgg ccttcctggg gagacggcaa caatgttgag ctcgacgtgg
     2161 aagtcgcgaa gatgaccgaa aatgcagtat ttttccagac ggcggtcacg cttctgtcca
     2221 cgaatatccg gatgtacaaa tcggcgttga agaggtaacc gtgaactcat tcgatgtgct
     2281 aaagatcagc ggatcggccc tcgaggcgca gcggcggcgg atgaacgtca tcgcttcgaa
     2341 cctggcgaac gcgaactcga cgagaacgga ggagggtggc ccctacaagc gacaggacgt
     2401 cgtcttcacc tccacgaccg tcgagtcgaa cccgttggag ctcaaaggcg tcaaggtcgc
     2461 agacgtcgtc cccgacggga aaccgccgaa gagtgtctac gatcccggcc accccgacgc
     2521 ggacaaggac ggcttcgtcg cgatgccgaa catcaacacg atggaggaaa tggtcaacat
     2581 gatgatggct ttccgagcct acgaagcctc cgttgcgacc ttcaacacgg ccaaaaccat
     2641 gctgctcaag acgctggaac tggggagagt ctgatgacgg acatgagaat cgcggggatc
     2701 gggggtgccg gaattctgac ccctgtcaag aatccggaca aaatcgccgg cggcgggttc
     2761 gacaatatcc tcaaagacgc cgtggggaag atctcccagg ttcagaagga tgccgaaacc
     2821 gcggtccggg aactctcctc gggcggggat gtgacgagcg ccgtcctctc gatggaaaag
     2881 gcggacatga ccttccagct gatggtcgag gtccggaacc gcctgctcac ggcctacgaa
     2941 gagatcatga gaatggcgat ctaaccgtaa ggggaaacga tgccgaccag tatgcccaat
     3001 gtgatcgacg acgtcatggc gtggcccgtt cgcaagaagg cgaccggtgt cgccgtaatc
     3061 gtcgcctccg tcgctctcct catcctctcg ttctcgtggt cccagaagga ggagtaccat
     3121 gtcctctact cgaacctgtc cgagtcggac gccggccagg tcgtccagaa gctgaaggaa
     3181 atgaaagtgc cctaccgggc cgatgcaggc ggaatcttcg ttccggccga ccgggtctac
     3241 gatgcgcggc ttcagctggc gtcccagggg cttccccagg gaggcggggt cgggttcgaa
     3301 ctcttcgaca agacaagctt cggcacgacg gatttcgtgc agaagctcaa ctacaaaaga
     3361 gcgcttcagg gcgaactcgc gcggaccgtc atggcgatcg gcgcggtgga gcagtgccgg
     3421 gtccacctcg cgattcccga gaagtcgctg ttcgcgcggg aaggagagca cgagcggccc
     3481 accgcctccg tgctcgtgaa actccggcaa ggcaaaacct tgtcctccgc ccaagtggac
     3541 ggta
//
LOCUS       DASVJL010000412         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_724750, whole genome shotgun sequence.
ACCESSION   DASVJL010000412 DASVJL010000000
VERSION     DASVJL010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724750"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..997
                     /locus_tag="VF357_08690"
     CDS             128..997
                     /locus_tag="VF357_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX7524266.1"
                     /translation="MENGKKVLVVGGGTMGQGIALVCALSGYGTTVVDLPAALPRTRA
                     GIEKSLADRLKKGRMSAAEMQGFLSSIRMEEGLDAAADADWIIEAIVETLDAKKALFA
                     EIEKRMKVDAVVATNTSSLSVTAMAAGMRNPGCVVGLHFFNPATVMPLVEIVRTCWTD
                     EAQLKKSFEFVTSIRKSPVLVKDTPGFIVNRIARSFAVESLKIVTEGIATPRQVDRIM
                     KLGGNFKMGPFELMDMVGIDINFAVTQSIFRAFFEEPRFRPQLLQQELLNAGRLGRKT
                     GHGVYEYETGGKK"
     gene            994..1755
                     /locus_tag="VF357_08695"
     CDS             994..1755
                     /locus_tag="VF357_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7524267.1"
                     /translation="MSATTKVAVVPGPGTPKELSTLLGNKGVTEWDPARVEKSGALDS
                     EAGIVFLFVDAGPAEQKHWLAALGRGGSKDAAVALYAPALSISELAMAYPWPERVVGF
                     GFLEQMLSPGSLIEIASGLRTRPEAADEVEAFLKAQGFETERVGDFSGLVLRRVLFMI
                     INEAAYALWEGIATREDIDLAMKLGTNYPMGPLEWADAIGLDRVYNGLKSLHEEYGDD
                     RYRPCPLIRKLVMSGFTGKRAGRGFYAHNGEGVTA"
     gene            1752..2540
                     /locus_tag="VF357_08700"
     CDS             1752..2540
                     /locus_tag="VF357_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7524268.1"
                     /translation="MSDAASTMLLKEDRGAVRILKLNRPEVRNALNDDLRTAVVREVR
                     TAETDPSVRAIVLTGEGPKSFCAGADIHALILRTPQAMLGQLQADRIDFILERSTKPV
                     VAAINGYAFGGGFELALACTIRIASENATVGLPEVNLGIFPSLGGTQRLPRLVGVGRA
                     MEMILSARILTAQEALEFGIVSRVVPREKLLDEAVELAGTIALKGPLAVRLAKDAILN
                     GFGMPIEEGLRYENRLAAVVMGTKDKKEGLSAFLEKRKPVFTGE"
     gene            2591..>3094
                     /locus_tag="VF357_08705"
     CDS             2591..>3094
                     /locus_tag="VF357_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7524269.1"
                     /translation="MNNVWKNVGLAAVLAVVTCGLAEAAPPIHLGVHEPLTGSLAKSG
                     AEKIKGIRLGAEDFERTSGIKVELYVYDDESNPQKAVAAVEKLAGVEKVHAIVGGFGS
                     NLVGPASEAAERYDTPYMTTGAVDTKLSAMRFKNFFRLNYMPGYAAAQSGTIRDLFKA
                     KKVGILYN"
BASE COUNT          581 a          904 c         1104 g          505 t
ORIGIN      
        1 gccctgtccg cctcccgtta cggctacgtc ctggagatgg gccgcattgc gctggaggga
       61 gaggcgtcgg cgctccgcga aaacccccac atcaagcagg cctacctggg cctataggag
      121 aaggtcgatg gagaacggga agaaggtgct ggtggtcggc gggggaacga tggggcaggg
      181 gatcgccctg gtctgcgccc tctccgggta cgggacgacg gtggtggacc tccccgcggc
      241 cctcccccgc acccgcgcgg ggatcgagaa gtccctcgcc gaccgactca agaaagggag
      301 gatgtcggcg gcggaaatgc aggggttcct ttcctccatc cgcatggagg aggggctgga
      361 cgcggcggcg gacgccgact ggatcatcga ggcgatcgtg gaaaccctcg acgcgaagaa
      421 ggcgctcttc gcggagatcg aaaaacgcat gaaggtcgac gcggtggtcg ccacgaacac
      481 ctcttccttg agcgtgacgg cgatggcggc gggaatgcgc aaccccgggt gcgtcgtcgg
      541 actccacttc ttcaaccctg ccaccgtgat gccgctcgtc gagatcgtgc ggacctgctg
      601 gacggacgag gcgcagctga agaagtcctt cgagttcgtc acgtcgatcc ggaagtcccc
      661 ggttctcgtg aaggacaccc cggggttcat cgtaaaccgg atcgcccgat cgttcgcggt
      721 cgagtcgctc aagatcgtga ccgagggaat cgccacgccg cgccaggtcg accggatcat
      781 gaagctgggg ggaaacttca agatgggccc cttcgaactc atggacatgg tggggatcga
      841 catcaacttc gcggtgaccc agagcatctt ccgcgccttc ttcgaagaac cgcgcttccg
      901 gccccagctc ctgcagcagg aacttttgaa tgcgggacgg ctcgggcgca agacggggca
      961 cggggtctac gagtatgaga cgggagggaa gaaatgagcg cgacgacgaa agtcgccgtg
     1021 gtccccggcc cggggacccc caaggagctc tcgacgctcc tgggcaacaa gggggtaacc
     1081 gagtgggacc cggcgcgggt ggagaagtcg ggggcattag actccgaggc ggggatcgtc
     1141 ttcctcttcg tcgacgcagg tccagccgaa cagaaacatt ggctcgccgc cttggggcgc
     1201 ggcggatcga aagatgccgc ggtggccctc tacgcgccgg ccctctccat ctccgagctg
     1261 gcgatggcgt acccctggcc ggagcgcgtg gtgggctttg gtttcctcga gcagatgctg
     1321 tcgccaggga gcctgatcga gattgcatcg gggctgcgca cgcggcccga ggcggccgac
     1381 gaggtcgagg cgttcctgaa ggcgcagggg tttgagacgg agcgtgtagg ggatttctcg
     1441 ggattggtcc tgcggcgggt tctcttcatg atcatcaacg aggccgccta cgccctttgg
     1501 gaggggattg caacccgcga ggacatcgac cttgcgatga agctcggcac gaactacccg
     1561 atggggcctc tggagtgggc cgacgcgatc ggcctggacc gggtgtacaa cggcctcaag
     1621 agtctccacg aggagtacgg cgacgatcga taccgcccct gcccgctgat ccggaagctg
     1681 gtgatgtcgg ggttcaccgg caagcgggcc ggccgcggat tctacgccca caacggcgag
     1741 ggggtgacgg catgagcgat gcggcgtcga cgatgctgct gaaggaggac cgcggagcgg
     1801 tccgcatcct gaagctcaat cgtccggaag tgcgcaacgc cctcaacgat gacctacgga
     1861 ccgcggtggt cagggaggtg cggaccgcgg aaaccgaccc gtccgtccgt gcgatcgtgc
     1921 ttaccggcga ggggccaaag tctttctgcg cgggggcgga cattcatgcc ctgatccttc
     1981 gaacacccca ggcgatgctc ggtcagctcc aggccgatcg gatcgacttc atcctcgagc
     2041 ggagtacgaa acctgtggtg gcggcaatca acggttatgc ctttggcggg ggattcgagt
     2101 tggccctggc gtgtacgatc cggatcgcct cggagaacgc caccgtcggg ttgcccgagg
     2161 tgaaccttgg gatcttcccc tccctcgggg gaacgcagcg gctcccacgg ttggtcggcg
     2221 tgggaagggc gatggagatg atcctgtcgg cccggatcct cacggcgcag gaagcgctgg
     2281 agttcgggat cgtgtcccgg gtcgtcccac gggagaagct cctcgacgaa gcggttgagc
     2341 tggccgggac gatcgcattg aagggacctc tggcggtgcg cctcgcgaag gatgcgatcc
     2401 tcaacggctt cgggatgcct attgaagaag ggttgcgata cgagaaccgg ctggctgcgg
     2461 tcgtgatggg gacgaaggac aagaaagaag ggctttcggc gttcctcgaa aagcgcaagc
     2521 ccgtgttcac aggcgagtga cggctcgaga tgcccgggcg acattgagag ccggtccgga
     2581 aagggggggc atgaacaacg tgtggaagaa cgtcgggctc gccgccgtgc tggcggtggt
     2641 gacgtgcggg ctggccgaag cggcgccacc catccatctt ggggtccacg aacctctgac
     2701 ggggagcttg gccaagagcg gagccgaaaa gatcaagggg atccgcctgg gtgcggagga
     2761 cttcgagcgg acgagcggga tcaaggttga actttatgtg tacgacgacg agagcaaccc
     2821 gcagaaggcc gtcgcggcgg tggagaagct cgccggcgtc gagaaggtac acgcaatcgt
     2881 cggtgggttc ggctcgaacc tcgtggggcc ggcctcggag gcggcggagc ggtacgacac
     2941 gccgtacatg acgaccgggg cggtggacac gaaactctct gcgatgaggt tcaagaactt
     3001 cttccgcctc aactacatgc cggggtacgc cgcagcccag tccggcacga tccgtgacct
     3061 cttcaaggcg aagaaggtag gtatcctcta caac
//
LOCUS       DASVJL010000413         4526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_68937, whole genome shotgun sequence.
ACCESSION   DASVJL010000413 DASVJL010000000
VERSION     DASVJL010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68937"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VF357_08710"
     CDS             <1..738
                     /locus_tag="VF357_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX7524270.1"
                     /translation="FHAKDENSWKLRFHTQTAGCTLTAQQPMNNVVRVALQALSGILG
                     GTQSLHTNSMDETMALPTEQSVTVALRTQQIIAEESGVANTIDPLGGSFFVEQLTNGM
                     EEKATEYIRKIDDMGGMVAAIKQGYPQREVADAAFHFQRLVDAGKKRIVGLNSYRSQE
                     EAPIPLLKIDDRVEKQQVARTKEVRRKRNAKKARACLSALKEGSLTPNVNLMPLLVDA
                     SREYVTLGEMCDTLRETMGVYTDPAMF"
     gene            765..1211
                     /locus_tag="VF357_08715"
     CDS             765..1211
                     /locus_tag="VF357_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX7524271.1"
                     /translation="MAMAKGKSVASTPKKAARKVRILVGKPGLDGHDRGAKIIARALR
                     DAGVEVIYTGLHQTPEMIVSAAAQEDVDGIGLSILSGAHNYLFPRIIKLLSEKKMGDV
                     VLFGGGIIPDDDIPKLVKKGVDRLFTPGTPIQEIVDYVNERIRPRK"
     gene            1267..2553
                     /locus_tag="VF357_08720"
     CDS             1267..2553
                     /locus_tag="VF357_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524272.1"
                     /translation="MRPVYMVSGGVSKFAKARPDATFQKIVKESFDYAMNDVPKLKHS
                     MIDGSVVSYFSDHFTRQLMAGIMATDYLALCPKPNKRVEGGGATGGLCFQAGWEAVAS
                     GRMNVCAAFGFETMSRVPTWKGNEFIALASDVNFDYPVGGFYSGYYAMMVNRHMHEFG
                     TTVEQLAMVSVKNHMNAYGNPYAQKRRKLTIADVRNSAMVAYPLTLLDICVMSDGAAT
                     CILADEETAFKLTDHPVKITGVGTGTDMMRMADRPHGDVLLAPNEKKSDYRNLKYPGV
                     HSFRAGRSAGLQAYKMAGITDPLKQIDFVELHDAYTSSEIQTYEDLALCKYGDGGKFV
                     EAGYPFMPQIDYGMKLPMKGTLPVNPSGGLIACGHPVGATGLMQAVFAFWQIQGSIKK
                     HYGNGELQLKKANRGLIHSHAGTGTYVTVSILERGW"
     gene            2556..3104
                     /locus_tag="VF357_08725"
     CDS             2556..3104
                     /locus_tag="VF357_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX7524273.1"
                     /translation="MAKKNPTITPAESKKVEELMTGTTVFNIPLPADLKAMKGMSPIV
                     IKQPYHIDYIHSYGQDSPFFAGLSNGKLLGTKCGRCDYTYATPRLACTHCGRETKWVE
                     IPLSGRVHTFTTCYFGGEEFLKETPFHLVLVEFDGVDTLFLSRLIGPVGPEDIRIGMK
                     VKAKFRRNSQLKPTDVYFVPTE"
     gene            3109..4254
                     /locus_tag="VF357_08730"
     CDS             3109..4254
                     /locus_tag="VF357_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7524274.1"
                     /translation="MEFVLNDEQQALRDMLRTFVAKEVRPKAREWDATAEFPLESVAK
                     LGKLGLLGAMVPEQYGGSGMDTVSYAVAVEEISKGDGSLGLTVASHNSLCTAHILGFG
                     SEAVKRKYLPELASGRTLGAWALTEPGSGSDSLAMRTKAEWKKDRWVINGSKMFITQG
                     NVAGVYVVLAVTDRDKGKDGVTAFVFPAGTKGLSIGKKLHKLGMRSSDTAELVFEDLE
                     VGPDAVVGQVNSGFRDTLKNLAGGRISIAALSVGIGLGAMREALAYSKERVQFGQAVS
                     EFQAIQWMFADMGTELEAAELMTFRAAALKDAGRPYIRDAAMAKLFASEAAIRATIKA
                     VQVFGGYGYTQEYPAERYMRDAKLCEIGEGTSEVQRIIIARDLIRSH"
     gene            4260..>4526
                     /locus_tag="VF357_08735"
     CDS             4260..>4526
                     /locus_tag="VF357_08735"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEX7524275.1"
                     /translation="MNAAGREILAGNTRAAARLMRDLDDEIPAAIRTLKYLYRHTGRA
                     YILGVTGAPGAGKSTLVNRITTHLRKRGKSVGIVAIDPTSPYSGG"
BASE COUNT          933 a         1340 c         1533 g          720 t
ORIGIN      
        1 ttccacgcga aggacgagaa ttcgtggaag ctccggtttc acacccagac ggcgggatgc
       61 acgctcaccg cgcagcagcc gatgaacaac gtggtccgtg tcgccctcca ggcgctctcg
      121 gggatcctcg gggggacgca gtcgctccac accaactcga tggacgagac gatggcgctt
      181 cccaccgagc agtccgtgac cgtcgcgctg cggacccagc agatcatcgc cgaggagtcg
      241 ggcgtcgcca acaccatcga tcctctggga ggatcgttct tcgtggaaca gttgacgaac
      301 gggatggaag agaaggcgac ggagtacatc cggaagatcg acgatatggg cgggatggtg
      361 gccgcgatca aacaggggta tcctcagcgg gaggtcgccg acgcggcctt tcatttccag
      421 cggctggtcg acgcaggaaa gaagcggatc gtcggcctca actcgtaccg gtcccaggag
      481 gaggccccga tccccctgct gaagatcgac gatcgcgtgg agaagcagca ggtcgcgcgg
      541 acgaaggaag tccggcggaa gcggaacgcg aaaaaggcgc gggcgtgcct gtccgcactg
      601 aaggaaggat ccctcacccc gaacgtgaac ctgatgcccc tcctggtgga cgcatcgcgc
      661 gagtatgtca ccctcggcga gatgtgcgac acccttcggg agacgatggg cgtgtacacc
      721 gatccggcga tgttctgaga gcgaaagatc gaggaggagg agcgatggcg atggcaaagg
      781 gaaaaagcgt ggcgtcaacc ccgaagaagg cggcccggaa agtccgtatc cttgtcggga
      841 aaccggggct cgacggccac gatcgggggg cgaagatcat cgcgcgcgcc cttcgcgacg
      901 ccggcgtgga ggtcatctac acaggtctgc accagacgcc cgaaatgatc gtgagcgcgg
      961 cggcgcagga ggacgtcgac ggcatcggcc tgtcgatcct ctccggggcg cacaactacc
     1021 tgtttccgcg gatcatcaag ctgctcagcg agaaaaagat gggcgacgtg gtgctcttcg
     1081 gcggcgggat catcccggac gacgacatcc cgaagctcgt gaagaagggg gtcgaccggt
     1141 tgtttacgcc ggggacaccg atccaggaga tcgtcgacta cgtgaacgaa cggatccggc
     1201 cgcggaaatg aggcggcagg accggcgcga agggaaaggg attccatacg acaaggggga
     1261 tcgaacgtga gaccggtcta catggtttcc ggcggcgtga gcaaattcgc gaaggcccgc
     1321 ccggacgcga cgttccagaa gatcgtgaag gagtcgttcg actacgcgat gaacgacgtt
     1381 ccgaagctca agcactccat gatcgacggg tcggtggtct cctacttctc cgaccacttc
     1441 acccgccagc tcatggcggg gatcatggcg accgactacc tggccctgtg cccgaagccg
     1501 aacaagcggg tcgagggcgg cggtgccacc ggcggtctct gtttccaggc ggggtgggaa
     1561 gcggtcgcct cgggaaggat gaacgtctgt gcggcgtttg gcttcgagac gatgtcccgc
     1621 gtcccgacat ggaaagggaa cgaattcatc gctctggcgt ccgacgtgaa cttcgattac
     1681 ccggtgggcg gcttctactc gggatactac gcgatgatgg tgaaccggca catgcatgaa
     1741 ttcggcacca cggttgagca gctcgcgatg gtgtcggtga aaaaccacat gaacgcctac
     1801 ggcaacccgt acgcccagaa acgtcgcaag ctgaccatcg ccgacgtgag gaactccgcc
     1861 atggtcgcct acccgctgac ccttctcgac atctgcgtga tgtccgacgg cgcggcaacc
     1921 tgcatcctcg ccgacgagga gacggcgttc aagctcacgg accaccccgt gaagatcacc
     1981 ggcgtgggga cgggcaccga catgatgcgc atggcggatc ggccgcacgg cgacgtgctc
     2041 ctcgccccga acgagaaaaa gagcgactac aggaatctga aatatcccgg cgtccattcc
     2101 ttccgggcgg gacgcagcgc ggggctccag gcgtacaaga tggcggggat cacggacccc
     2161 ctgaagcaga tcgacttcgt cgaactgcac gacgcctaca cctcctccga aatccagacg
     2221 tacgaagacc tcgcactgtg caagtacggc gacggcggga agttcgtgga ggcggggtac
     2281 cccttcatgc cgcagatcga ctacgggatg aagctcccga tgaagggaac gcttccggtg
     2341 aacccatcgg gtgggctgat cgcctgcggt cacccggtgg gcgcgacggg actcatgcag
     2401 gccgttttcg ccttctggca gatccagggg agcatcaaga agcattacgg caacggggaa
     2461 cttcaactga agaaggccaa tcgcggactg atccacagcc acgcgggcac ggggacctac
     2521 gtgaccgtgt cgatcctgga acgggggtgg tgaccatggc gaagaagaat ccgacgataa
     2581 cacccgcgga atcgaagaag gtcgaagaac tgatgaccgg gacgacggtc ttcaacattc
     2641 cgttgccggc cgacttgaag gcaatgaagg ggatgtcccc gatcgtcatc aagcagccgt
     2701 atcacatcga ttacatccac tcctatgggc aggactcgcc gttcttcgcg gggctttcga
     2761 acgggaagct cctcgggacg aagtgcgggc ggtgcgacta cacctacgca accccgcgcc
     2821 ttgcgtgcac ccattgcggg cgggagacca agtgggtcga aatcccccta tcgggacggg
     2881 ttcacacctt caccacctgc tatttcgggg gagaagagtt cctgaaggag acccccttcc
     2941 acctcgtgct ggtggaattc gacggggtcg atacgctctt tctttcccgg ctgatcggcc
     3001 cggtggggcc cgaagacatc cggatcggaa tgaaggtgaa ggcgaagttc cgccgcaatt
     3061 cccagctgaa accgaccgat gtctatttcg tgcccacgga gtagggggat ggagttcgtc
     3121 ctgaacgacg agcagcaggc gttgcgggac atgctgcgga cgtttgtcgc gaaggaggtc
     3181 cggccgaagg cgagggagtg ggacgccacg gccgagtttc ctctggaatc cgtcgcgaaa
     3241 ctcggcaagc tcggtctcct cggagcgatg gtccccgagc agtacggcgg atccgggatg
     3301 gatacggtca gctacgccgt cgccgtggaa gagatctcga agggggacgg ctcgctcggc
     3361 ttgacggtgg cgtcccacaa ctctctttgc accgcccaca tcctcggatt cgggtcggag
     3421 gcggtgaagc ggaaatacct tcccgagctg gcgtccggaa ggaccctcgg ggcctgggcc
     3481 ctcaccgagc ccggctccgg ttccgactcc ctcgcgatgc ggacgaaagc ggaatggaag
     3541 aaagaccgct gggtgatcaa cggaagcaag atgttcatca cccagggaaa cgtcgccggg
     3601 gtatacgtgg tcctcgccgt gacggacagg gacaagggga aagacggcgt caccgccttc
     3661 gttttcccgg ccgggacgaa ggggctctcg atcgggaaaa aactccacaa gctcgggatg
     3721 cgctcctcgg ataccgccga actggtcttc gaagatctcg aggtggggcc cgacgccgtg
     3781 gtggggcagg taaactccgg attccgggac acgttgaaaa acctcgccgg gggacggatt
     3841 tcgatcgcgg ccctctcggt ggggatcggc ctcggggcga tgcgggaggc gctggcctat
     3901 tcgaaggagc gggtccagtt cgggcaggcg gtttcggagt tccaggcgat ccagtggatg
     3961 ttcgcggaca tggggaccga gctcgaggcc gcggagttga tgacgttccg ggcggcggcg
     4021 ctcaaggacg ccgggcgccc ttacatccgg gatgcggcga tggccaagct gttcgcctcc
     4081 gaggcggcga tacgggcgac gatcaaggcg gtccaggtgt tcggagggta cgggtacacg
     4141 caagagtatc ccgcagagcg gtacatgcgg gatgcgaagc tgtgcgagat cggggaaggg
     4201 acctccgagg tgcagcggat catcatcgcc cgcgacctga tccggagtca ttgatcgcca
     4261 tgaacgccgc cggaagggag atcctcgccg gaaacacccg ggccgcggcg cggctgatgc
     4321 gggacctgga cgacgagatc ccggcggcga tccgcactct gaagtacctg taccggcaca
     4381 cagggcgcgc ctacatcctc ggcgtgacgg gggcgcccgg cgcgggaaag tcgaccctcg
     4441 tcaaccggat caccacgcat ctgcggaagc gcgggaagtc tgtcgggatc gtcgcgatcg
     4501 acccgacgag tccttactcc ggcggc
//
LOCUS       DASVJL010000414         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1007597, whole genome shotgun sequence.
ACCESSION   DASVJL010000414 DASVJL010000000
VERSION     DASVJL010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007597"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..989)
                     /locus_tag="VF357_08740"
     CDS             complement(306..989)
                     /locus_tag="VF357_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7524276.1"
                     /translation="MRKPLALVLAGLFVAATAATSVAADYDRYERGPRGGPPGEYAPP
                     PPRRGAEAPPPRHAQYGQPYFFGHLGIFDPNSDVDGLDRYDSGMNFDLGVGSRVSPVF
                     AIDGTFGAYGAKHGGDKASVVPLTIGGRLILPHPFIEPYVGGGLGLYFSSLKEGSRTG
                     FSGIDDSSADFGGYLSAGLDMWLNSRTALNFEGKYHWVNPTFQTNAGNDIDVNMSGWT
                     VNLGVRVGF"
     gene            1131..2150
                     /gene="aroF"
                     /locus_tag="VF357_08745"
     CDS             1131..2150
                     /gene="aroF"
                     /locus_tag="VF357_08745"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX7524277.1"
                     /translation="MIIVMGAGAAQKEIRTVIARIKALGYTPHPIFGKERTVIGAIGD
                     ERGKNVLQGLESLPGVERVVPILKPYKLASREVKPERTVIRIAPGVTIGDRQLLVIAG
                     PCSVESETQMIDTALAVKKAGAHVLRGGAWKPRTSPYAFQGLELKGLKILRKAGDRAG
                     MPIITEVMNPADVARIAEYSDVLQVGARNVQNFSLLKRIGKSKRPILLKRGMMTTITE
                     FLMSAEYCLSEGSRKVILCERGIRTFEDATRNTLDLSAIPVLKERTHLPIIADPSHAT
                     GVARLVPPMSCAAVAAGADGLMIEVHPTPEKALSDGSQSLTFSKFAETMAMLQPFIAA
                     AGRTL"
     gene            2219..>3296
                     /locus_tag="VF357_08750"
     CDS             2219..>3296
                     /locus_tag="VF357_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX7524278.1"
                     /translation="MGRGVSGDAWEVPSILYGRPTEIRTGDHFGNLRFTERLRRLSYK
                     KVAGKPSVAGTWSEEPDRVRIYSRDYRIEETPHNGGPVEIGVRDGRVVSIASSAGVPL
                     DSIHLEPEEIGRILGPRMESRRIVPLSAIPPSLQQAVLAAEDSRFYSHHGIDAVGIAR
                     ALVRNLRERRFAQGGSTITQQLAKNFFLSPKKTIGRKLREAELALALELRYPKKTILE
                     MYMNKIYFGQEGARGIYGVEEAAGFYFSKRAADLPLEEAATLAGVIRSPNRYSPFRAP
                     AAAKERRNTVLARMRQLGMIGEEEYRRASHAPIRTRARRSPTNMAAYFADYIQRVTED
                     DLGGEKLFRTGYRFYTTLDPVQQAA"
BASE COUNT          573 a         1113 c         1094 g          516 t
ORIGIN      
        1 cttccgggag atggcgccgg gccccgtcca ggcgacgatc cgtgtcaccc ccagggattt
       61 cgcggcgtag atgttggcgc gatagttcac atatggtgcg gtcttctcgt actccttctc
      121 gccgtgccgc gagaggaaga ggaagcggaa accggcgttc tcgcagaggt ggacggggtt
      181 ggaaaggccg tacggcgtcc ggacgcgccg ggaagagagc ctctccccca gcgtgcccgg
      241 agggagggcg taggccccgg atcctccgag gaggaggacc cggggcgctt cttccctgcc
      301 gtttctcaga aaccgacccg gacgccgaga ttgacggtcc agccgctcat gttcacgtcg
      361 atgtcgttcc ccgcgttggt ctggaaggtc ggattgaccc agtgatattt cccctcgaag
      421 ttgagcgccg tccgggagtt gagccacatg tcgagaccgg cggagagata gccgccgaag
      481 tcggcgctgg agtcgtcgat ccccgagaat cccgttctcg aaccctcttt cagactcgag
      541 aagtagaggc ccagcccgcc cccgacgtac ggctcgatga acgggtgggg caggatcagg
      601 cgtcctccga tcgtcaacgg gaccacgctc gccttgtccc ccccgtgttt cgcgccgtag
      661 gcgccgaagg tgccgtcgat ggcgaacacg ggcgacacgc gggaaccgac cccgaggtcg
      721 aagttcatcc cggagtcgta ccgatccaac ccgtcgacgt cgctgttggg atcgaagatg
      781 cccaggtgcc cgaaaaagta cggttggccg tactgcgcat gccggggcgg tggcgcttcg
      841 gcaccccgcc gcggcggggg cggcgcgtac tccccggggg gccctccacg ggggccccgt
      901 tcgtaccggt cgtaatcggc ggcgacgctt gtcgcagcgg tcgccgcgac gaacaatccg
      961 gcgagaacca acgcgagagg cttcctcatg atgctgtcct ccttctttct ccggcgaacc
     1021 cggaggtgga atcgaggata ccgcccgtcg aagtggaaag aaagggtcgg aatccatata
     1081 atgatgcatc ctctgtacac aaatatacga cgctgcgacg ggagatattc atgatcatcg
     1141 tcatgggagc gggtgcggcc caaaaagaga ttcgcaccgt catcgccagg atcaaggcgc
     1201 tggggtacac cccgcacccg atcttcggga aggaacgcac cgtcatcggc gcgatcgggg
     1261 acgagcgcgg caagaacgtc ctccaggggc tcgagtcgct ccccggggta gagcgggtcg
     1321 tcccgatcct caagccgtac aagctcgcca gccgcgaggt gaagccggaa cggacggtca
     1381 tccgcatcgc ccccggggtg acgatcgggg accgtcagct cctggtcatc gcgggcccct
     1441 gctccgtgga aagcgaaacg cagatgatcg atacggcgct ggccgtcaag aaggcgggcg
     1501 cccacgtgct gcgcggcggg gcgtggaagc cgcgcacctc cccgtacgcc ttccaggggt
     1561 tggaactcaa ggggctcaag atcctgcgca aggcgggaga ccgcgcgggg atgccgatca
     1621 tcaccgaagt gatgaacccc gccgacgtgg cgcggatcgc ggaatattcg gacgtcctcc
     1681 aggtcggggc gcgcaacgtc cagaacttct ccctcctcaa gcggatcggc aagtcgaagc
     1741 gccccatcct cctcaagcgc gggatgatga cgacgatcac cgagttcctg atgagcgcgg
     1801 aatattgcct gtccgagggg agccgcaagg tgatcctgtg cgagcgcggg atccggacct
     1861 tcgaggacgc cacgcggaac accctcgacc tctccgcgat cccggtcctc aaggagcgca
     1921 cccacttgcc gatcatcgcc gacccgtccc acgccaccgg cgtggcgcgc ctcgtcccgc
     1981 ccatgtcgtg cgccgccgtc gccgcgggcg ccgacggact gatgatcgag gtccacccca
     2041 cgccggagaa ggcgctctcc gacggctcgc agtcgctgac cttttcgaag ttcgccgaaa
     2101 cgatggcgat gctccagcct ttcatcgcgg cggcggggcg aacgctctga tccgcaggat
     2161 cctccttccg gcgctggcgg cggtcctcct cctccacttc ggctactcgt actaccaggt
     2221 ggggcggggg gtctccgggg acgcttggga ggtcccctcc atcctctacg gacgccccac
     2281 ggagatccgc acgggggacc acttcgggaa cctccggttc accgagcggc tgcggcgcct
     2341 ctcctacaaa aaggtcgcag ggaaaccgtc cgtcgccggg acctggtccg aggaacccga
     2401 ccgcgtccgg atctactcgc gcgactaccg gatagaggaa actccccaca acggcggccc
     2461 ggtggagatc ggggtccgcg acgggcgggt cgtttcgatc gcctcgtccg ccggggtccc
     2521 gctcgattcg atccacctcg agccggagga gatcggtcgg atcctcggcc cccggatgga
     2581 atcgcggcgg atcgtccccc tgtccgcgat cccgccgtcg ctgcagcagg ccgtgctcgc
     2641 cgccgaggac tcccggttct actcccacca cggcatcgac gcggtcggga tcgcccgcgc
     2701 gctcgtgagg aatcttcggg agcggcggtt cgcccagggg gggtccacga tcacccagca
     2761 gctggcgaag aacttcttcc tctccccgaa gaagacgatc ggacggaagc ttcgcgaggc
     2821 ggagctcgcc ctcgctctcg agctgcggta cccgaagaag acgatcctcg agatgtacat
     2881 gaacaagatc tatttcggcc aggagggggc ccgcggaatc tacggcgtcg aggaggcggc
     2941 gggcttctac ttctcgaagc gcgcggcgga cctccccctc gaggaggccg cgacgctggc
     3001 cggcgtcatc cgctcgccga accggtactc cccgttccgc gccccggcgg ccgcgaagga
     3061 gcgccgcaac acggtgctcg caaggatgcg ccagttgggg atgatcgggg aggaggagta
     3121 ccgcagggcc tcccatgcgc cgattcggac gcgggcgcgg cgctccccga cgaacatggc
     3181 cgcgtacttc gccgactaca tccagagagt caccgaggac gacctggggg gggagaagct
     3241 cttccgcacc ggctaccgct tctacaccac cctcgatccg gttcagcagg ccgcgg
//
LOCUS       DASVJL010000415         3348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_444827, whole genome shotgun sequence.
ACCESSION   DASVJL010000415 DASVJL010000000
VERSION     DASVJL010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444827"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VF357_08755"
     CDS             <1..201
                     /locus_tag="VF357_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7524279.1"
                     /translation="GLGLPIVHAIVEKHGGTIHLDNREGAGVTFSVFLPLFPKEGGTG
                     ERILEQLRKGGTNGNGPAANKG"
     gene            170..1267
                     /gene="mqnE"
                     /locus_tag="VF357_08760"
     CDS             170..1267
                     /gene="mqnE"
                     /locus_tag="VF357_08760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEX7524280.1"
                     /translation="METDRRRIRDKVEEGERLSEADALALYRARDILAVGEMAALANR
                     RANGDRVYFIVNRHVNPTNICVNRCRFCAFSKGKDDPLAYTMTMDEILHRAEEAQEQR
                     ATELHIVGGLHPDLPFDFYLTMLRSLRERFPSLHVQAFTAVEIDYFRKITGLPLAEVI
                     AQLKDAGLGSLPGGGAEIFAPEIRNEICPEKISGDQWLEVMEAVHAAGLRSNATMLYG
                     HVETLESRVDHMRRLRELQDRTGGFQSFIPLAFHPKNTEIAKGYTTGLEDLLALAVAR
                     LYLDNFRHIKSFWIMVGPKLAQISLHFGVDDIDGTVVEEKITHAAGAQTGQEMSVAEL
                     VTMIRQAGKIPVERDTLYNMVREWPAEGARG"
     gene            1264..2379
                     /gene="mqnC"
                     /locus_tag="VF357_08765"
     CDS             1264..2379
                     /gene="mqnC"
                     /locus_tag="VF357_08765"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEX7524281.1"
                     /translation="MNGSGADRREAVERASLGLVSVAEGVRLLREAPLFELARAADAI
                     RRKLHPDGIVTYIVDRNINYTNVCSCGCSFCAFYRKKDAPDAYVLSGEELSAKIVDTI
                     EAGGVRVLLQGGLHPDWGIDEAVRLVRAVKRHPILLHGFSPPEVWHFAHQSKITIGEV
                     IARLKGAGLDSIPGGGAEILDDEVRRRISPKKIGWEQWAAVMREAHRQGLRSSATMMF
                     GSVESPEAIVAHLLRVRALQEETGGFTAFIPWVFQPGNTELSRDPRFGEASALGFGHA
                     VGYLRVLALSRILLPSVPTVQVSWVTMGAKVAQIGLFFGADDFGSTMMEENVVASAGV
                     SFRMSEEEIVATIRDAGFTPVHRPGLFGAGRRETGAA"
     gene            2384..>3348
                     /gene="serS"
                     /locus_tag="VF357_08770"
     CDS             2384..>3348
                     /gene="serS"
                     /locus_tag="VF357_08770"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX7524282.1"
                     /translation="MLDLKFVRENIAIVEEALRKRRSSLTLDGFVALDKARRERLVEV
                     EGMRAERNAASEEIGRLRREKKDASELMARMKALSLLLKEAEAELPTIEGEMEDLLLA
                     IPNVPDLSVPDGAGEEDNPVVRTWGTPRAFSFPVKDHVDLGAALDILDFDRAVKITGG
                     RFCLSKGAGALLERALINFMLDVHTREHGYLEVLPPFMANSASFFGTGQLPKFEEDLF
                     RVAGTDYFLVPTAEVPVTNIVRDEILAAETLPLKMTAYTPCFRAEAGSYGKDTRGMIR
                     QHQFNKVEMVKICRPEESAQELEKLTDDAEEILRRLDLPYRVVTL"
BASE COUNT          576 a         1033 c         1208 g          531 t
ORIGIN      
        1 ggcctcgggc ttcccatcgt ccacgccatc gtcgagaagc acggcgggac gatccatctg
       61 gacaaccggg agggggccgg tgtaaccttc tcggtcttcc tccccctctt cccgaaggag
      121 gggggaaccg gggagcggat cctcgagcaa cttcgcaagg gcgggacgaa tggaaacgga
      181 ccggcggcga ataagggata aggtcgagga gggggagcgg ctgtcggagg ccgacgcgct
      241 cgcgctctat cgcgcgcggg acatcctcgc cgtcggggag atggcggccc tggcgaaccg
      301 acgggcgaac ggcgaccgcg tctacttcat cgtcaaccgg cacgtgaatc cgacgaacat
      361 ctgcgtgaac cggtgcaggt tctgcgcgtt cagcaagggg aaggacgatc ctcttgcgta
      421 cacgatgacg atggacgaga tcctccaccg cgcggaagag gctcaggagc agcgggccac
      481 ggaacttcac atcgtcgggg ggttgcaccc ggacctcccc ttcgacttct acctgacgat
      541 gctgcgctcc ctgcgggaac ggttcccttc tctgcacgtc caggcgttca ccgcggtgga
      601 gatcgattat ttccggaaga tcaccgggct tccgctggcc gaggtgatcg cgcaattgaa
      661 ggatgcgggc ctcggatccc ttcctggcgg cggggcggag atcttcgccc cggagatacg
      721 gaacgaaatc tgtcccgaga agatttcagg ggaccaatgg ctcgaggtga tggaggcggt
      781 ccacgcggcg ggacttcgaa gcaacgcaac gatgctctac ggccacgtgg agacgctgga
      841 gtcgcgggta gaccacatgc ggcgcctgcg ggagctgcag gaccgcaccg gcgggttcca
      901 gtcgttcatc cccctggcct tccacccgaa gaacacggag atcgccaagg ggtacaccac
      961 ggggctcgaa gacctcctcg cgctggcggt ggcgaggctc tacctggaca acttccgcca
     1021 catcaagtcg ttctggatca tggtgggacc gaagctggcg cagatctccc tccacttcgg
     1081 ggtggacgac atcgacggga ccgtggtcga ggagaagatc acccacgcgg ccggggcgca
     1141 gacggggcag gagatgtccg tcgccgagct cgtgacgatg atccgccagg cgggaaagat
     1201 ccccgtcgag cgggacaccc tctacaacat ggtccgcgag tggccggccg agggggcccg
     1261 ggggtgaacg gatccggggc ggatcggagg gaagcggtcg agcgggcatc gctcgggctc
     1321 gtatcggtgg cggagggagt ccggctgctg cgggaggcgc cgctgttcga gctggcgcgg
     1381 gcggccgacg cgatccggcg gaaactgcac ccggacggca tcgtcaccta cattgtcgac
     1441 aggaacatca actacacgaa cgtctgctcc tgcggctgct ccttctgcgc cttctaccgg
     1501 aagaaggacg cacccgacgc ctacgtcctc tccggggagg agctctccgc caagatcgtc
     1561 gacacgatcg aggcgggcgg ggtccgcgtg ctgctgcagg gggggctgca ccccgactgg
     1621 gggatcgacg aggcggtccg gctggtgcgc gcggtcaagc gccacccgat cctcctccac
     1681 ggtttctccc cgccggaagt gtggcacttt gcgcatcagt cgaagatcac gatcggcgag
     1741 gtcatcgcgc gcctcaaggg cgccggcctc gattcgatcc ccggcggcgg ggcggagatc
     1801 ctcgacgacg aggtgcggcg ccggatcagc ccgaagaaga tcggttggga acagtgggcg
     1861 gcggtgatgc gcgaggcgca ccggcagggg cttcggtcct ccgcgacgat gatgttcggc
     1921 agcgtggaga gccccgaggc gatcgtcgcc catctgctcc gggtccgcgc gctccaggag
     1981 gagacggggg gcttcaccgc gttcatcccc tgggtgttcc agccggggaa taccgagttg
     2041 tcgcgcgacc cgcggttcgg ggaggcgtcg gccctgggat tcggccacgc cgtggggtat
     2101 ctgcgggtgc tggcgctgtc ccggatcctg ttgccgagcg ttccgaccgt ccaggtgtcg
     2161 tgggtgacga tgggggcgaa ggtggcgcag atcgggctct tcttcggcgc ggacgatttc
     2221 ggcagcacga tgatggaaga gaacgtggtc gcctccgcgg gcgtctcctt ccggatgtcc
     2281 gaggaggaga tcgtcgccac gatccgggac gccgggttca caccggtcca ccgcccgggg
     2341 ctgttcgggg cgggaagacg agagacggga gcggcctgaa aagatgctcg acctgaaatt
     2401 cgtccgggaa aacatcgcga tcgttgaaga agcgctgagg aagcgccggt cctccctcac
     2461 cctcgacggc ttcgtggcgc tggacaaggc gcggcgggaa cggctcgtcg aagtcgaggg
     2521 gatgcgggcg gagcgcaacg cggcttcgga ggagatcggc cgcctgcgcc gggagaagaa
     2581 ggacgcgtcg gagctcatgg cgcggatgaa ggcgctctct ttgctgctca aggaggcgga
     2641 agcggaactt ccgacgatcg agggggagat ggaggacttg ctcctcgcca tcccgaacgt
     2701 gccggacctc tcggtcccgg acggcgcggg ggaggaggac aaccccgtcg tgcggacgtg
     2761 ggggaccccg cgggccttct ctttcccggt caaggaccac gtcgacctcg gcgcggcgct
     2821 ggatatcctc gacttcgacc gggcggtcaa gatcaccggg ggccgcttct gcctgtcgaa
     2881 gggggcgggt gcgctgctgg agcgggcact catcaacttc atgctcgacg tccacacgcg
     2941 ggagcacggc tatctcgagg tgcttccccc cttcatggcg aacagcgcgt cgttcttcgg
     3001 caccgggcaa cttcccaagt tcgaggagga tctcttccgc gtggcgggga ccgactactt
     3061 cctcgttccc accgcggagg tgccggtgac gaacatcgtc cgggacgaga tcctcgccgc
     3121 cgagacgctt cccctgaaaa tgacggcgta caccccgtgc ttccgggcgg aggcggggtc
     3181 ctacgggaag gatacgcggg ggatgatccg tcagcaccag ttcaacaagg tggagatggt
     3241 caagatctgc cgcccggagg agtccgcgca ggagctggag aagctcaccg acgatgccga
     3301 ggagatcctt cggcgcctcg acctgccgta tcgcgtggtg acgctgtg
//
LOCUS       DASVJL010000416         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_445061, whole genome shotgun sequence.
ACCESSION   DASVJL010000416 DASVJL010000000
VERSION     DASVJL010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445061"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VF357_08775"
     CDS             <1..325
                     /locus_tag="VF357_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524283.1"
                     /translation="AIFSEDGSLLYWSYQTDRSEGEWPTLGKGAWILRSRIPPRMLNE
                     GSYRVELIGTLHRRIWLFEPGVNAPGVYMTIWGGLSDSPYWMERRPGLFAPVIEWTAS
                     PAEGA"
     gene            330..1169
                     /locus_tag="VF357_08780"
     CDS             330..1169
                     /locus_tag="VF357_08780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7524284.1"
                     /translation="MGGDGQAMTVSVVIPTWNRAATLEAAVRSALAQNVPPLEVLVCD
                     DGSTDGSEGVVRAISEPRVRWVPGRRGGRPAIPRNRGIREARGDWVAFLDSDDEWLPE
                     KLRKQLSQAAGSGCRAACSNARRRVPGAATAGLLLADLPDRVTFDDLLQVNHVVCSSV
                     IAEKSIVLRASGFPESPELKAIEDYALWLRIATITDFTYSREPLLWYNDDPAASLRRD
                     DPDVRVQRSRVLADFRSWVEGGGAPASFLAKASQSLADREEKSILARFAAFARRSACR
                     MRP"
     gene            1214..>2377
                     /locus_tag="VF357_08785"
     CDS             1214..>2377
                     /locus_tag="VF357_08785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7524285.1"
                     /translation="MQEVVRQLSERLAAMGHDVTVATSKMEGRSGTVLNGVRIREFDV
                     SGNLVEGMRGDVSGYREFLRGGGFDVMTNFAAQQWATDAAIAILDAIPSRKVLVPTGF
                     SRLYFPGYRNYFRSMKEWMRQYDVNVFLSETYLDAAFAREHGIDNRVLIPNGAGEDEF
                     LRKSGIDVRDRLGIPPDAFLVLHVGSHTGDKGHREALEIFRRARVRHAVFLIVGKETG
                     GCNAECHATATNQNATREFLSAGKRVIVRSLSREETVAAFRSADLFLFPSNIECSPLV
                     LFEAAASGTPFLATDVGNAVEIAEWTSGGMILPTDKSEETEKKEGGLLLRVRRLSSVL
                     LSFLSPPIPPGHSRARTEEAARVLESLYASPDERRRMAEAGSEAWKSRFTWEKI"
BASE COUNT          401 a          716 c          823 g          437 t
ORIGIN      
        1 cgccatcttc tccgaggacg gatcgctcct gtactggtcg taccagaccg accggtccga
       61 gggggagtgg cctaccctcg ggaagggcgc ttggatattg cggagcagga tccctcccag
      121 gatgttgaac gaggggtcgt accgtgtcga actgatcggg acgctccatc gcagaatatg
      181 gttgttcgag cccggcgtga acgcgccggg ggtctacatg acgatctggg gggggctgag
      241 cgattcccct tactggatgg agcgccggcc agggttgttt gcccctgtga ttgaatggac
      301 ggcttctccg gcggaaggtg cgtgaggccg tggggggtga cggccaggcg atgacggtct
      361 ccgtggtcat ccccacctgg aaccgcgcgg cgactctcga agccgccgtg cggagcgccc
      421 tcgcccagaa tgtgccgccc ttagaggtcc tggtgtgcga cgacggttcg acggacggga
      481 gcgagggggt ggtgcgggcg atcagcgaac cccgtgtgcg atgggtcccc gggcgacgcg
      541 gcggcaggcc cgccatcccg aggaaccgcg ggatccgcga ggcccggggg gattgggttg
      601 ctttcctcga cagtgacgac gagtggttgc cggagaaact ccggaagcag ctgtcgcagg
      661 cggcggggag cggctgccgg gcggcgtgtt cgaacgcccg ccgcagggtc ccgggtgcgg
      721 ccaccgcggg cctgcttctc gcggatctgc ccgaccgggt tacgtttgat gatctcctgc
      781 aagtgaacca cgtggtgtgc agttccgtca tcgcggagaa atccattgtc ctgcgcgcct
      841 ccgggttccc cgaatccccg gaactcaagg cgatcgagga ttacgccttg tggttgcgaa
      901 tcgctacgat caccgatttt acctactccc gcgagccgct cctctggtac aacgacgatc
      961 cggcggccag cctgcggagg gacgaccccg acgtccgggt ccagcggtcg agggtgctgg
     1021 cggatttccg gtcctgggtg gaaggcggcg gagctccggc ttcgttcctt gcgaaggcct
     1081 cccagagcct tgcggatcgc gaagagaaga gcatcctcgc ccggttcgcc gcgtttgctc
     1141 gtcgatccgc atgccgcatg cgcccgtgag gatactccac actgtcgagt cttaccctcc
     1201 atccgtgggg gggatgcagg aggtcgtgcg ccagctctcc gagcgacttg cggcgatggg
     1261 gcacgatgtc acggtggcca cgtcaaagat ggagggcaga agcgggacag tcctcaacgg
     1321 cgttcggatc cgcgagtttg acgtgtccgg caacctcgtc gaggggatgc ggggcgacgt
     1381 ctccggctac cgggaatttc tccggggcgg gggcttcgac gtgatgacga acttcgcagc
     1441 gcaacaatgg gcaaccgacg ccgcaatcgc gatcctcgac gcgataccgt cgaggaaggt
     1501 ccttgtgccg accggtttct cccggctcta ctttcccggg tatcggaact atttccgttc
     1561 gatgaaggaa tggatgcgcc agtacgatgt gaacgtgttc ctgtcggaga cgtacctcga
     1621 tgccgcgttc gcccgggagc acgggatcga taaccgggta ctgatcccca atggcgccgg
     1681 agaggacgag tttcttcgta agagcggaat cgatgtccgg gaccgcctcg gtatcccgcc
     1741 ggacgcgttc ctcgtcctcc acgtgggttc ccatacgggg gacaaggggc accgggaggc
     1801 cttggagatc ttccgcaggg cacgtgtccg gcatgcggtg ttcctcattg tcgggaagga
     1861 gacgggtgga tgcaatgcgg agtgtcacgc cactgcgacg aaccaaaacg caacgaggga
     1921 attcctatcc gcgggaaaac gcgtgatcgt ccggtccctt tcccgggaag agacggtcgc
     1981 cgcttttcga tcggccgacc tcttcctgtt tccttcgaac atcgaatgct cgccgttggt
     2041 cttgttcgag gccgccgcct cgggaacccc cttcctggcg acggacgtcg gcaacgctgt
     2101 ggagatcgcg gaatggacct ctgggggaat gatccttccc acggataaga gtgaggaaac
     2161 ggagaagaag gaaggaggtc tcctgctgcg cgtccgccgg ctctcctccg tgcttctgtc
     2221 gttcctttcc ccgccgatcc cccccggcca ctccagagcg aggacggagg aggcggcgag
     2281 agtattggag agcctctacg ccagccccga tgagcgcagg cgcatggcgg aagctggcag
     2341 cgaagcctgg aagagccggt tcacctggga gaagatc
//
LOCUS       DASVJL010000417         3183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_725580, whole genome shotgun sequence.
ACCESSION   DASVJL010000417 DASVJL010000000
VERSION     DASVJL010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725580"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VF357_08790"
     CDS             complement(<1..1097)
                     /locus_tag="VF357_08790"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7524286.1"
                     /translation="MTLLTDLIARNEDHLLAQLLSLAEANGYFRFVPGTPEEWRATLR
                     GLSGSLLQAFRSDPSPPALTADDVGHEDGLSAFGVVEARKRRREGMPFGTFLGLVKLF
                     RQAYIDLVRTAGLPREEEEGHLRYIERFFDRNEAAVCVSWTSESVSARLAELRQEHDE
                     LSRVYSLVATAKKEWEGTIDRVDDMLFLADPGRRIRRCNRTFREFVGRPYGEILGQTF
                     DRLLSEAGVDVEPSFERPVECFHERTGKWFVVSHYPFKDVSGGGTQATIVTVHDSTEL
                     KKAAEELSRKNLRLSEALAALKRSQAKVLHQEKMASIGQLAAGVAHEINNPIGFINSN
                     LSTLGKYLSRLSGFLTVQSECIAAGAPSEQV"
     gene            complement(1199..1945)
                     /locus_tag="VF357_08795"
     CDS             complement(1199..1945)
                     /locus_tag="VF357_08795"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7524287.1"
                     /translation="MRQQESVPVPRGWTRGAAWKGAGASLLLHLFVGAAMIVALSGPP
                     KAPPQVIDLSLLPPAGISLRPVPPTFIAAVKPSRAPGPGTAVLPEPSALPASQPVENP
                     PDETAPASPSDPAVKKSAAVPGPPHAFPAVPVSTGQGPPVKAAAGRPTGISGAGPPAA
                     GFAWIRDAIQHAIAYPATARRMGWEGEVVVAFQLLSDGSVRDVRVVQGSGHAALDRGA
                     IEAVRNASPFHRSPVEAEVITPVVYRLTTR"
     gene            complement(1970..3145)
                     /locus_tag="VF357_08800"
     CDS             complement(1970..3145)
                     /locus_tag="VF357_08800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7524288.1"
                     /translation="MERAKLHRNVVAIGLVSLLTDLSSEMIYPLLPVFRTVTLAAGPA
                     VLGLIEGVAETTASLLKLFSGAWSDRSGRKKPLVLAGYGLSTLARPLVGFATGWGHVL
                     AVRFCDRIGKGVRTSPRDALVAAYVPAAQRGKAFGLQRSMDHLGAVLGPLAAFLLLAG
                     GISLRSVFLLSILPGLAAVAVLALLVRDAPAAGAPPHAGSLLRRDGLSPAFRRYLLLV
                     GLFTLGNASDAFLILRAVDSGVPVRYVPLLWGAFHVVKSSLSVPAGALSDRIPRKWVV
                     AGGWCVYATCYAAFSIVRGAAATVAVFLLYGLYAAACEGAERAMVADFVPADRRGTAF
                     GWFHLVTGICALPASVLFGLLWNAYGAPAAFGVSAGLALLAAALLLLLRPEKYERIR"
BASE COUNT          525 a         1075 c         1132 g          451 t
ORIGIN      
        1 tcgacctgtt ccgacggcgc gccggcggcg atgcactcgg actggacggt gaggaacccg
       61 gagagtcgcg acagatattt cccgagggtg gagaggttgc tgttgatgaa gccgatcggg
      121 ttgttgatct cgtgggccac accggcagcg agctgcccga tcgaggccat cttctcctga
      181 tggaggacct tggcctggct gcgcttgagc gcagccagcg cctcgctcag ccggaggttc
      241 ttccgggaca actcctcggc cgccttcttc agctctgtcg agtcgtggac cgtgacgatc
      301 gtcgcctggg tcccgccacc ggagacatcc ttgaacgggt agtggctgac cacgaaccat
      361 ttcccggtcc gctcgtggaa gcattccacc ggtcgttcga aggagggctc cacatcgacg
      421 ccggcttccg aaaggagccg gtcgaacgtc tgcccgagga tctccccgta cggccgcccg
      481 acgaactccc tgaacgtccg gttgcaccgc cggatccgcc gccccggatc ggccaggaag
      541 agcatgtcgt cgacgcggtc gatcgtcccc tcccactcct tcttggccgt cgcaaccagg
      601 ctgtagacgc gggagagttc gtcgtgctcc tgccggagtt ccgcgagccg cgcggatacg
      661 ctctccgagg tccacgacac gcagacggcg gcttcgttcc ggtcgaagaa ccgctcgatg
      721 taccgaaggt gcccctcttc ctcctcgcgc gggagtccgg cggtccggac gagatcgatg
      781 tacgcctggc ggaataattt gacgagcccg aggaaggtcc cgaaaggcat cccctcgcgg
      841 cggcgtttgc gcgcctcgac gacgccgaac gcggagagtc cgtcctcgtg gccaacgtcg
      901 tcggcggtga gcgcgggagg ggaggggtcg gagcggaacg cctggagcag ggagccggac
      961 agcccgcgga gcgtggcacg ccactcctcg ggagtcccgg ggacgaaacg gaaatatccg
     1021 ttcgcctccg cgagggagag gagctgcgcg agcaggtggt cctcgttccg ggcgatgaga
     1081 tcggtcagca gcgtcacgcc gtgatccccc tggagctcaa tccatggttc cctttgccgt
     1141 ttccgggagc cgatggatct catcggaaga acctgcagaa ttctttacgg tttccgtgtc
     1201 aacgcgtcgt cagccgatag acgaccgggg tgatgacctc cgcctcgacg ggggaccggt
     1261 ggaacgggga ggcatttcgg accgcctcga tcgctccccg gtccagggcg gcgtgccccg
     1321 agccttgcac gacccgcacg tcccggaccg agccgtccga aaggagctgg aaggccacca
     1381 cgacctcccc ctcccacccc atcctgcgcg ccgttgccgg gtaggcgatc gcgtgctgga
     1441 tggcgtcgcg gatccaggcg aagcccgccg ccggaggccc tgcgcccgat atccccgtcg
     1501 gtctcccggc ggctgccttg acgggaggcc cctgtcctgt cgataccggg acggcgggaa
     1561 aggcatgcgg aggtccggga acggcggcgc tctttttcac cgccgggtcg gacgggctcg
     1621 ccggcgcggt ctcgtcgggc ggattctcga cgggttgcga ggcgggaagg gcgctcggtt
     1681 ccgggaggac ggccgtcccg ggtccggggg cccttgacgg tttcacggca gcgatgaagg
     1741 tgggcgggac cgggcgcagg gagattccgg cggggggcag gagagagagg tcgatcacct
     1801 gcgggggcgc cttcggggga ccggagagtg cgacgatcat cgccgcgccg acgaagaggt
     1861 gcaggaggag ggacgctcct gcccctttcc aggcggctcc gcgcgtccag ccccgcggca
     1921 cggggacgct ctcctgctgc ctcatcacca ctccctcttg tgccgattct tatcggattc
     1981 tctcatactt ttccgggcgc aggaggagca ggagcgctgc ggcgaggagg gcgagccccg
     2041 cgctgacgcc gaaggccgcg ggggcgccgt aggcgttcca caggagaccg aagaggacgc
     2101 tcgcggggag ggcgcagatg ccggtgacga gatgaaacca gccgaacgcc gtcccccgcc
     2161 ggtcggccgg gacgaaatcg gccaccatcg cccgctcggc cccctcgcac gccgccgcgt
     2221 agagtccgta cagcaggaag acggcgaccg ttgcggccgc gccgcgcacg atggagaagg
     2281 ccgcgtagca ggtggcgtac acgcaccagc cgcccgccac cacccacttc ctggggatcc
     2341 ggtccgagag ggcgcccgcg gggacggaga gggaagattt caccacgtgg aacgcccccc
     2401 acagcaacgg gacgtaccgc acgggcactc cggaatccac ggcccgcagg atgaggaagg
     2461 cgtcgctcgc gttcccgagg gtgaacaggc cgacgaggag caggtaccgg cggaacgccg
     2521 gcgaaagccc gtcgcggcgc agcaagctcc ccgcgtgggg cggcgccccc gcggcggggg
     2581 cgtcgcggac caggagcgcg aggacggcca ccgccgccag gcccgggagg atcgacagga
     2641 ggaagacgga acgaagggag atcccgcccg ccaggaggag gaacgccgcc aggggcccga
     2701 gcaccgcccc gagatggtcc atcgaccgct gcagcccgaa cgcctttccc ctttgtgcgg
     2761 cggggacgta cgcggcgacc agcgcatccc tcggggaggt ccgcacccct ttcccgatcc
     2821 ggtcgcagaa ccgcacggcg aggacgtgtc cccaccccgt cgcgaatccg accaacggcc
     2881 gcgcaagggt ggagaggccg tacccggcga gaaccagcgg tttcttcctt ccgctccggt
     2941 cggaccaggc gccggagaag agcttgagca ggctcgccgt cgtctccgcc accccttcga
     3001 tcagtccgag gacggcgggg ccggcggcca gggtgacggt gcggaagacc ggaaggagcg
     3061 ggtagatcat ctcgctcgac agatcggtga gcaggctcac cagcccgatg gcgacgacgt
     3121 tccggtgcag cttcgcgcgt tccatcaaac cctcaatgat gggccgtacg ggcgcgcaac
     3181 agg
//
LOCUS       DASVJL010000418         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_351444, whole genome shotgun sequence.
ACCESSION   DASVJL010000418 DASVJL010000000
VERSION     DASVJL010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351444"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..>1792
                     /locus_tag="VF357_08805"
     CDS             20..>1792
                     /locus_tag="VF357_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX7524289.1"
                     /translation="MSRRILTGWVFDVIPDAHGMTVWFRTDGGETVPLSAPFHPSFVL
                     AGRNLKETNLRTVSVRWNCSLTRGEGTEFFSGKTIPAWTFTVAAPALLSPTVRKAEKA
                     FGPEALFDADIAPEQQFAYPTGLYPLSRAAIDCEGNGTIRFSRLLDSPWDVDAPLPSL
                     STALLRMEGTGHPAHGRVRSLEFVADGITNSLEWEDGAEFLRSLLRLLEETDPDLLVT
                     EYGDDWLLPQLLALASRLRVPFPLGRPRLRAADDSIRRTKERSYLSYGRVVFLAAPHT
                     LSGRWHVDARNSFLFGETGLPGLIELSRLSGIPLQRVARTSPGTAISAMQVATALRRG
                     ILVPYKKREPEEFKSGIDLVVADKGGLTYIPRPGMHENVGELDFASMYPSLMDRYNIS
                     PETINCTCCRPGRLVPEIGMHTCERRRGLVPDTIAPILAKRRLLKERQKAAQDPEARD
                     LFKKRQTALKWLLVVCFGFLGYRNARFGRIEAHEATTAWGREKLLLAKEIAEREGFAF
                     LHGLTDAIWVKQDGAQEEEYQQLADRITEETGMPIALEGVYNWIAFLPSKRNPTVAVP
                     NRFAGAFSTGEIKVRGIALRRSDTP"
BASE COUNT          317 a          587 c          570 g          318 t
ORIGIN      
        1 atgccgaggg gcaatcggaa tgtcccggag gatcctgacc ggctgggtct tcgacgtgat
       61 ccccgacgcc cacgggatga ctgtatggtt tcgcaccgac ggtggggaaa cggtccccct
      121 gtccgcaccc tttcatccat ctttcgtcct cgcggggaga aacctcaagg aaacaaacct
      181 tcggacggtt tctgtgcgct ggaactgctc cctcacgcgg ggagagggca ccgagttctt
      241 ctccggcaag acgatcccgg cgtggacgtt cacggtcgcc gcgccagccc tcctgagccc
      301 caccgtccgt aaagcggaaa aggcgttcgg gccggaagcg ctgttcgacg cggatatcgc
      361 cccggagcag caattcgcct accccacagg gctctacccc ctgtcgcggg ccgctattga
      421 ttgcgaaggg aatggtacga tccgtttctc ccggcttctt gactccccgt gggacgtgga
      481 tgcgccgctc ccctcccttt cgacggccct gctccggatg gagggaaccg gtcacccagc
      541 gcacggaagg gtgcgctcgc tggagttcgt cgccgacggg atcacaaaca gtctcgagtg
      601 ggaagacggg gcggagtttc tacggagtct tctacggctc cttgaggaaa ccgaccccga
      661 cctgctcgtg accgaatatg gggacgactg gctcctgccg cagttgctcg ccttggccag
      721 ccggctccgt gtcccctttc cccttgggcg tccccggctc agggcggcgg acgactcgat
      781 ccggcgcacg aaggagaggt cgtacctttc ctacggccgc gtggtcttcc ttgcggcgcc
      841 ccacacgctc tccggccgtt ggcacgtcga cgcccgcaac tccttcctct tcggagagac
      901 cggcctcccg gggttgatcg aactttcccg cctctccggc attccattgc agcgcgtcgc
      961 tcgtacatcg ccggggacgg ccatttcggc gatgcaggtg gcaaccgccc tgcgccgcgg
     1021 gatcctggtg ccgtacaaga agcgggagcc cgaggagttc aagtccggga tcgacctcgt
     1081 cgtggccgac aaggggggac tcacctacat tccgcgcccg gggatgcacg agaacgtcgg
     1141 agaactagac ttcgcctcga tgtacccgtc gctgatggac cggtacaaca tctccccgga
     1201 gacgatcaac tgcacctgct gccggccggg tcggctcgtc ccagagatcg gcatgcacac
     1261 gtgcgaaaga cgccggggtc tcgtccccga taccatcgct ccgatcctcg ccaagcgccg
     1321 actcctgaag gagcggcaga aggcagcgca ggacccggag gctcgtgatc ttttcaagaa
     1381 gcggcagaca gccctcaagt ggctcctcgt cgtctgcttc ggattcctag ggtatcggaa
     1441 cgcccggttc ggccggatcg aggcgcacga agccacgacg gcgtgggggc gggagaagct
     1501 gttgttagcc aaggagatcg cagagcgcga agggttcgcc ttcctccacg gcctgacgga
     1561 tgcgatatgg gtgaagcagg atggcgccca ggaagaggag taccagcagc tcgcggatcg
     1621 gatcaccgaa gagacgggga tgccgatcgc actggaaggg gtctacaact ggatcgcctt
     1681 tctcccgtcg aagcggaatc caacggtggc cgtaccgaac cggttcgcgg gggcattttc
     1741 cacaggggaa atcaaggttc gcgggattgc tctacgccgc tccgacaccc cg
//
LOCUS       DASVJL010000419         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_445391, whole genome shotgun sequence.
ACCESSION   DASVJL010000419 DASVJL010000000
VERSION     DASVJL010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445391"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1338)
                     /gene="rnd"
                     /locus_tag="VF357_08810"
     CDS             complement(160..1338)
                     /gene="rnd"
                     /locus_tag="VF357_08810"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX7524290.1"
                     /translation="MEKEPKGRVVTDPAEFRDLVAAMRTSGKVGFDTEFVGERTYFPR
                     LCLIQLGTETMSVAVDPLAVGDLSPLDDLLFDASVLKLVHAGWQDLKIIHQRTGRVPA
                     PVFDTQIAAALLGLPAQAAYASVVHEFLGVEVKKGHSYSDWAARPLSPSQLAYALDDV
                     RYLPALHDRMVERLAREGRLAWIEPEIATLSSPASYETEPQLEYRRVKGWTALDRRRL
                     AVLRALIAWREGEARRRDVPRRRVLADESALAIARSKPANEEALRRVRGLEWKSGGGS
                     SSAVLEMVRDALALPEAQLPEAPPSRPRGNGDRASVVGLLGALVRSRSRVHRVAPEVL
                     TNTDELERLAAGEREGIAVLSGWRLDLLGRELLALLDGRISLVIHRGNVVVEERDVTK
                     "
     gene            complement(1352..>2002)
                     /locus_tag="VF357_08815"
     CDS             complement(1352..>2002)
                     /locus_tag="VF357_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX7524291.1"
                     /translation="APLGHDVQEISDAGGSPICVAVDGKAAGVLSVADEVKDGAPEAI
                     RRLCEMGLDVVMLTGDNRRTAKAVAEKVGISRVIAEVLPDRKAQEIRALQAEGKVVAM
                     AGDGINDAPALAQADVGIAMGTGADIAVEAGDLVLMGGELRGVADAISLSRATLRNIR
                     QNLFWAFAYNVVLIPVAAGVLYPSFRLLLNPVYAAAAMGLSSVTVVANALRLRRFR"
BASE COUNT          310 a          752 c          646 g          294 t
ORIGIN      
        1 ccgaggattg gacggctccg gcctccttcc ccgcgctcac gcccgccgcg catcctccct
       61 gcgtgaagag gacgccgccc gtcagcaggg cggcggcgat aagggagaac ccttttttca
      121 ccacggtaac gtggacgtag ggcgtattca tgaaacgcgt cacttggtga cgtccctctc
      181 ctcgacgacc acgtttcccc gatggatcac gagggagatc cgcccatcga gcagggccag
      241 caattccctc ccgaggagat cgagccgcca ccccgatagc accgcgatcc cttcccgctc
      301 cccggccgcc agccgctcca gttcgtcggt attcgtcagc acctccggtg caacccggtg
      361 cacccgcgac cgggaacgca cgagcgcgcc caggagcccc acaacggagg cgcggtcccc
      421 gttgccgcga ggccgtgacg ggggggcctc cgggagttgc gcctccggca aggcgagggc
      481 gtcccgcacc atctccagga cggccgacga cgacccgccc ccgctcttcc actccagccc
      541 gcgcacgcga cgcagcgcct cctcgttcgc gggcttgctg cgggcgatcg ccagcgcgct
      601 ctcgtcggcc agcacgcggc gtcgggggac gtcccggcgc ctcgcctccc cctcccgcca
      661 cgcgatcagg gcgcggagca ccgccaggcg gcggcggtca agggccgtcc accccttgac
      721 gcgacggtac tccagctggg gctccgtctc ataggaggcg ggcgaggaca atgttgcgat
      781 ttcgggttcg atccaggcga ggcgtccctc ccgcgccagc cgttcgacca tacggtcgtg
      841 cagcgcgggg agataccgga cgtcgtccag ggcatacgcc agctgcgacg gggagagggg
      901 ccgggcggcc cagtcggagt aggagtgccc cttcttcacc tcgaccccca ggaactcatg
      961 aaccaccgac gcgtacgccg cctgggcggg aagcccgagg agggccgcgg cgatctgcgt
     1021 gtcgaagacc ggcgcgggca cccgtcctgt ccgctggtgg atgatcttca gatcctgcca
     1081 tccggcgtgc accagtttga ggactgaggc gtcgaagagc agatcatcga gcggcgacag
     1141 atcgccgacg gcgagggggt ccaccgcgac ggacatcgtc tccgtgccga gctggatcag
     1201 gcagagccgg gggaagtagg tccgttcccc tacgaactcg gtgtcgaatc cgacctttcc
     1261 ggatgtgcgc atcgcggcga ccaggtcgcg aaactccgcc gggtccgtca cgacccgccc
     1321 cttcggttcc ttctccattc cccctctttt cctagcggaa tcgacgcagg cgcagggcgt
     1381 tggcgacgac cgtcaccgag gagagcccca tcgcggcggc ggcgtacacc gggttcagca
     1441 gcagccggaa cgaaggatag agtactcccg cggcgaccgg gatgagaacc acgttgtagg
     1501 cgaacgccca gaacaggttc tgccggatat tgcgcagggt ggcgcggctc aaggatatgg
     1561 cgtcggccac cccccgcagc tcccctccca tcagcaccag gtcccccgcc tccacggcaa
     1621 tgtccgcgcc ggtccccatg gcgatgccga cgtcggcctg cgcgagcgcc ggggcgtcgt
     1681 tgatcccgtc ccccgccatc gcgacgacct tcccctccgc ctggagcgcg cggatctcct
     1741 gcgcctttcg gtccggcagg acctccgcga tcacccggga gatcccgacc ttttccgcga
     1801 cggccttcgc ggtgcgccga ttgtcccccg tgagcatgac gacgtccagg cccatctcac
     1861 acaggcgacg gatcgcctcc ggcgcgccgt ccttcacctc gtcggccacg gagaggacgc
     1921 ccgccgcctt gccgtccacc gccacgcaga tcgggctgcc gcccgcatcg gagatctcct
     1981 gcacgtcgtg accgagcggg gc
//
LOCUS       DASVJL010000420         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_915935, whole genome shotgun sequence.
ACCESSION   DASVJL010000420 DASVJL010000000
VERSION     DASVJL010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915935"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            512..>1718
                     /locus_tag="VF357_08820"
     CDS             512..>1718
                     /locus_tag="VF357_08820"
                     /inference="COORDINATES: protein motif:HMM:NF015555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524292.1"
                     /translation="MHEPLFTSCLRGAGYVIGLARMALDAAALRRGKDAPLAYPDTAY
                     ELPVVFGLTDIPVSTLADAGKVLDLAGTLVRNGATAENALDAGAATLLAAEVIGAISH
                     ENGNPFMPPAMGFVPDSILRKLGIYLVDGSVPGVAVVIGKATDPAAAAAMVRQFQEIG
                     LLIFLAGDVVSQLDAQGVKLGEDFKTFPLGPLPAVIHAVNFAVRAGLTFGGIGRGQGA
                     AMLSYLGNRVKAFVCALGPLDDIALAIAAGAMKAGLTVLSDQPVPEIPGLLLSRPAGD
                     GMVQAGMEARGIKVKMVKVPIPIAFGPAFEGERIRKADTHIEFGGGRSVAYELVRSAP
                     PEEVIDREIVVDGPDLRGLPIGSVLPLGIVVRVAGARMQHDFESVLERRIHRIVNYGE
                     GTMHVAQRDT"
BASE COUNT          298 a          569 c          567 g          284 t
ORIGIN      
        1 aggaatagca acgggcgtgc cacgggaaaa acctctggaa tccggtcact tcctcaggcg
       61 cagccaaaac agtacagata attatgtctt atttgcgaca ttcatggcgg actcaggaca
      121 ctccgcgccg aagagtgttc atcgccacca gcctccgcgc tgcagccatt tggggcaggg
      181 ctccgggctc gcacttagga ggcgcatcgg agcgggatat cccttccgag cgggcgtgtc
      241 ctcgccgatg gccagggatg gccaagtgcc gcgggagcca tggatggcgg gagcggccag
      301 tgaaggcaac cagggacggt tgccggaacg tagcgaggaa gggggtatcc cgcggagatc
      361 aaagcacctc cgtttacggt accgtattag cacgagcacc gaggcacaat aatcttgcgc
      421 cggggattaa tgtttgtgag ggcggcgcag gtgaattaca atcacttcct gttctcccgc
      481 catgacgtaa ccaccgaagg gagcccgccc gatgcacgaa ccgctgttta cctcctgcct
      541 gcgcggcgcc gggtatgtca tcggtctcgc ccggatggcc ctcgatgccg ccgccctccg
      601 ccggggtaaa gacgctccgc tcgcttaccc ggacaccgcc tacgaactgc ccgttgtctt
      661 cggtcttacc gatatcccgg tttcgactct ggccgacgcc gggaaagtgc tcgacctcgc
      721 ggggacgctc gtgaggaacg gcgccaccgc ggaaaacgcg cttgacgccg gtgcggccac
      781 gctcctcgcc gcggaagtga tcggcgccat ctcccacgag aacggcaacc cgttcatgcc
      841 accggcgatg ggcttcgtac ccgattcgat cctgcgcaaa ctcgggatct accttgtgga
      901 cggctccgtt cccggggtcg ccgtggtgat cgggaaggcg acggaccccg cggccgcggc
      961 ggcgatggtc cggcagttcc aggagatcgg gctcctcatc ttcctcgcgg gggacgtggt
     1021 ctcgcagctc gacgcgcagg gagtgaaact cggagaggat ttcaagacgt tcccgctggg
     1081 gccgctcccc gcggtgatcc acgccgtcaa cttcgccgtc cgcgcggggc tcaccttcgg
     1141 cggaatcggc cgcggccagg gagccgcgat gctctcttac ctcggcaacc gcgtgaaggc
     1201 gttcgtgtgc gccctcggac ccctggacga catcgccctg gcgatcgccg ccggagcgat
     1261 gaaggcgggg ctgaccgtcc tgtccgacca gcccgtgccg gagatccccg gactgctcct
     1321 ctcgcgtccc gcgggcgacg gaatggttca agcggggatg gaggcccgag gcatcaaggt
     1381 gaagatggtc aaggtgccga tccccatcgc cttcggtccc gccttcgaag gggagcggat
     1441 ccggaaggcc gacacgcaca tcgagttcgg cggcgggcgc tccgtggcct acgaactcgt
     1501 ccgcagcgcc cccccggaag aggtgatcga ccgggagatc gtcgtggacg gccccgacct
     1561 ccgtgggctg cccatcggga gcgtcctccc gctggggatc gtcgtccggg tcgccggcgc
     1621 gcggatgcag cacgacttcg aatcggtcct tgagcgccgc atccaccgga tcgtcaatta
     1681 cggcgaggga acgatgcacg tcgcccaacg ggacacca
//
LOCUS       DASVJL010000421         3968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_165037, whole genome shotgun sequence.
ACCESSION   DASVJL010000421 DASVJL010000000
VERSION     DASVJL010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165037"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..742)
                     /locus_tag="VF357_08825"
     CDS             complement(47..742)
                     /locus_tag="VF357_08825"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX7524293.1"
                     /translation="MASVRIRWFGHSGFSIRDGKTALIDPWFEGNPKTPDGVGSAPGA
                     DLLLLTHDHFDHAGDVVAMARKTGALVVAIFELAGDLKAKGIPEAQLLHGGGGMNVGG
                     TVSVQGFEITMTEARHSCALGAPVGYVLKTPSGLTIYHSGDTGIFAGMALIGELYPID
                     VAMLPIGSVFTMDHRQAAKACALLKTKAVIPMHYGTFPILEPNADRFVAELAKVSPGT
                     RPIVLSPGEETTV"
     gene            884..1936
                     /locus_tag="VF357_08830"
     CDS             884..1936
                     /locus_tag="VF357_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524294.1"
                     /translation="MKKARTAGPSRPFRVVVPNRVDIAGGTLDIYPLYLLIGNAMTVN
                     AAVAVRSEVAVRPFRRGAARMSSGNFGIAVTAVDTHCFSLGGKMGLLSRALRHFPAVS
                     GVEIRMSNEAPVGSGIGASSALLVALMLAAGRLTGTACRRDETALAAMEIEAAHLKSL
                     TGRQDHMAALRGGLQGIRFSAGRVDALRLPPGGEAGTMLRDHAFLAHTGIAHRSSDVN
                     WRMIRGAIDGDGIVLRKFRGIAAAARDAWEAVSAADPRGTGVAIAAEWAIRKTLAPGV
                     STAGVEELLPDRRFRRLVSGAKLCGAGGGGMLFGLLRDPDDREALEALLSGMGMSVLP
                     FRLSGGPRFEEIENGG"
     gene            1926..3296
                     /locus_tag="VF357_08835"
     CDS             1926..3296
                     /locus_tag="VF357_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7524295.1"
                     /translation="MAAEKTWDVAVIGAGPAGMFVARTLAGTLSVLVLDEKGRTGGAG
                     AMTDGKLNLSPHIGLDLAELQMTEEEADARIAAVDEVFVAHGADPTVYGEDRERIEAW
                     LDRVSWVRRQTPNGEWDITLRPARQRHMGTDLAHRVVARMTDSLTSAGAVFSLDTRVD
                     GISLGPGDLFALRTAKGELLARYVVAAPGRDGAYWFREAARGLGVTTRYGSIDIGCRV
                     EVASPVYDDITRVLYDPKFLFVTPTHGDRTRTFCTNPGGRVRVEARNGFQLVNGDALK
                     IRKTTNTNFAILNTVSMTEPIQDTTEMGRKVAEFANFWGGGESLVVQRLGDLMQGRRS
                     RSETFHSAGLGYDKMTPTLLPGPGVTPGDISFAYPGRIVDNLRETLTLLSRVIPGVAH
                     PSTAIYVPEIKFYDTKYVTDRYLETNVPNLFVAGDGVGKSRGIVGAALNGCLAAEGSL
                     RREGKS"
     gene            complement(3643..>3968)
                     /locus_tag="VF357_08840"
     CDS             complement(3643..>3968)
                     /locus_tag="VF357_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524296.1"
                     /translation="DSAILFAPAGELVPPALRALKKGGTLALAGIHMTDVPGMKYEEC
                     LFFEKNLRSVTANTRADGEGLLREAAEIPLRPKVTLFPLDDANRALQMLKADRISGSG
                     VLVVG"
BASE COUNT          634 a         1321 c         1367 g          646 t
ORIGIN      
        1 ggcttggata cgttgccgtg tttacgaacg agaccttccg gtttcttcag accgtcgtct
       61 cttcccccgg tgagagtacg atcgggcgcg tgccgggtga gactttcgcc agttcggcga
      121 cgaagcggtc cgcgttcggc tcgaggatcg ggaacgttcc gtagtgcatc ggaatcaccg
      181 ctttggtctt gaggagggcg cacgccttcg ccgcctgccg gtgatccatc gtgaacacgg
      241 acccgatcgg cagcatcgcc acgtcgatcg ggtacagttc gccgatgagc gccatgccgg
      301 cgaagatccc cgtgtcgccg gagtggtaga tggtgaggcc ggacggggtt ttcagcacgt
      361 agccgaccgg agcgcccagc gcacacgaat ggcgcgcctc cgtcatcgtg atctcgaacc
      421 cctggacgct gaccgtccca cccacgttca tcccgccgcc cccgtgaagc agctgggcct
      481 cggggatgcc tttcgccttc aggtcccccg ccaactcgaa gatcgccacg acgagggccc
      541 ccgtcttcct cgccatcgcg accacgtccc ccgcatggtc gaagtggtcg tgcgtgagga
      601 gcaacaggtc cgccccgggc gcggagccca ccccgtccgg cgtcttcggg ttcccctcga
      661 accacgggtc gatgagcgcc gtcttcccgt cccggatgga aaagccggag tgtccgaacc
      721 agcggatccg caccgatgcc atggccgccc tccttcgcgc gatgggaatt acagattctt
      781 atataccggt acggcgcccg atccgtaaag ggggaaatgc ctcctacccc gtggcgggag
      841 agcgtggtaa aacgacaatg gggatgcgac gaaggggggc agcatgaaaa aggcgaggac
      901 agcggggccc tcgaggccct tccgggtggt ggtccccaac cgcgtggata tcgcgggggg
      961 gaccctcgat atctacccct tgtacctcct gatcggaaac gcgatgacgg tgaacgctgc
     1021 cgtcgcggtg cgcagcgagg tcgcggtgcg cccgttccgg cgcggcgcgg caaggatgtc
     1081 ctccggaaat ttcgggatcg cagtcaccgc cgtcgacacc cactgcttct ccctcggagg
     1141 aaagatgggt cttctctccc gcgccctgcg gcattttccc gcggtttccg gcgtcgagat
     1201 ccgcatgagc aacgaagcgc cggtcggctc cgggatcggc gcgtcgtccg ccctgctggt
     1261 cgccctgatg ctggcggccg gaaggctgac cgggaccgcg tgccgcaggg acgagaccgc
     1321 cttggcggcg atggagatcg aggcggccca cctgaagagc ctgaccggcc ggcaggacca
     1381 catggcggca ctgcgcggcg gcctccaggg gatccggttt tccgccgggc gcgtcgatgc
     1441 gctccgcctc ccccccgggg gggaggcggg gacgatgctg cgggaccacg cgttcctcgc
     1501 ccacacggga atcgcccacc gctcgtcgga cgtaaactgg cggatgatcc gcggggcgat
     1561 cgacggggac ggcatcgtgc tgcggaagtt ccgggggatc gccgccgcgg cgcgggacgc
     1621 atgggaggcg gtgtccgcgg cggatccgcg aggcacgggc gtcgcgatcg ccgcggaatg
     1681 ggcgatccgc aagaccctcg cccccggcgt gtccacggcg ggcgtggagg aacttttgcc
     1741 cgaccgtcgg ttccgtcgcc tggtgagcgg cgcgaagctg tgcggcgcgg gcgggggagg
     1801 gatgctcttc ggcctcctcc gggacccgga cgatcgcgag gcgctcgagg cgctcctttc
     1861 cgggatgggg atgtccgtcc tcccgttccg gctctccggc ggtccgcggt tcgaggaaat
     1921 agagaatggc ggctgaaaaa acgtgggacg ttgccgtcat cggcgcgggt cccgcgggga
     1981 tgttcgtcgc ccggacgctg gccgggacgc tatcggtcct cgtcctcgac gagaaggggc
     2041 gcaccggcgg ggcgggcgcg atgaccgacg ggaagctcaa tctctctccc cacatcggcc
     2101 tggacctcgc cgaactgcag atgaccgagg aggaggcgga cgcgcgcatc gcggccgtcg
     2161 acgaagtttt cgtcgcccac ggcgccgatc ccacggtcta cggcgaggac cgggagcgga
     2221 tcgaggcgtg gctcgaccgg gtttcctggg tccggcgcca gactccgaat ggggagtggg
     2281 acatcaccct tcggccggcg cggcaacgcc atatgggaac ggatctcgcc caccgggtgg
     2341 tggcccggat gacggattcg ctcacgtccg cgggagcggt attctccctc gatactcggg
     2401 tcgacgggat ctccctgggg cccggcgatc tcttcgccct gcgcacggcg aagggggaac
     2461 tgctcgcgag gtacgtcgtg gccgcacccg gacgcgacgg cgcctactgg ttccgggagg
     2521 cggcccgggg gctcggggtc accacgcgct acggttccat cgacatcggc tgccgggtgg
     2581 aggtggcgtc cccggtctac gacgatatca cccgcgtcct gtacgatccg aagttcctct
     2641 tcgtgacccc cacccacgga gaccggacac ggaccttctg caccaacccg ggaggccggg
     2701 tacgcgtgga agcgcgcaac gggttccagt tggtgaatgg agacgccctc aaaatccgca
     2761 agacgacgaa cacgaacttc gcgatcctga acaccgtgtc gatgacggag ccgatccagg
     2821 acacgaccga gatggggcgg aaggtggccg agttcgccaa tttctgggga ggaggggaga
     2881 gcctggtcgt gcagcgcctg ggggatttga tgcaggggcg acgttcccgg agcgaaacgt
     2941 tccactccgc cggcctcggg tacgacaaga tgacgccgac gctgctcccc ggcccggggg
     3001 tgacgccggg ggatatctcc ttcgcctacc ctgggaggat cgtcgacaac cttcgggaga
     3061 ccttgacgct cctgtcccgc gtgatccccg gcgttgcgca cccctccacg gcgatctacg
     3121 tcccggagat caagttctac gacacgaaat acgtcaccga ccggtatctc gagacgaacg
     3181 tcccgaacct gttcgtcgcg ggcgacgggg tgggaaaatc ccgcgggatc gtcggcgccg
     3241 ccctcaatgg ttgcctggcg gccgagggga gcctgcgcag ggaagggaaa tcttaggact
     3301 tcagcgaata gtcccggtaa catgtgccgg tcggaaagtt ggactccggt ctcgcggggg
     3361 tgcttcctct ccgctacggc tcgtcgaccg tccatggtct ccagtcgctc cgccgcatcc
     3421 cagcggcccg cgacacttgg ccatccctgg ccatcgtcgc ctgcgccgcc gctctgcgga
     3481 accccgatgc ccatggacgg gcaagtgccg cgagagccat ggatggcggg agcggccccg
     3541 ctgcgccctg cgtccccgaa ctcaggaggt gcatcggaac ggggtatcac gcgaagataa
     3601 cagcgcctcc gtttacgttg ccgtatttat gaaacgcagc gctcatccga caacgagtac
     3661 cccggagccg gagatccggt ccgccttcag catctgaagc gcgcggttcg catcgtcgag
     3721 cgggaagagg gtgaccttcg ggcgcagggg gatctccgcc gcctcgcgca gaagcccttc
     3781 gccgtccgct cgggtgttcg ccgtcacgct ccgcaggttc ttttcgaaaa agaggcactc
     3841 ctcgtacttc atccccggca cgtcggtcat gtggatcccc gccagggcga gagtcccccc
     3901 ttttttcagc gcccgcagcg ccggcgggac gagctccccc gccggggcga agaggatggc
     3961 ggaatccg
//
LOCUS       DASVJL010000422         3343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_632411, whole genome shotgun sequence.
ACCESSION   DASVJL010000422 DASVJL010000000
VERSION     DASVJL010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632411"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VF357_08845"
     CDS             <1..354
                     /locus_tag="VF357_08845"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX7524297.1"
                     /translation="FTGLRPGEKLHEELIVEGEDVSGTTHPKVMKLIGNERIPSSWEK
                     RLEELIACAVTGERCGVVEKLDALVKGYRPNYEFHGVAVPEHARAASPGATSPAPPPE
                     GDLPPDPASPSKSVH"
     gene            complement(372..791)
                     /locus_tag="VF357_08850"
     CDS             complement(372..791)
                     /locus_tag="VF357_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524298.1"
                     /translation="MKAHDYRAIFDALSVKSQETIVAETSSALAAAAKQPRPGKPTPT
                     DTNPTTVKKPDPGRDAVRNDFVEGGPIAQAYWDAFLRRFDPDAALEHSRWEIGSVGKD
                     RAEILLTHHGADHPARLKMFREAGGWKAGLVETFWSR"
     gene            complement(961..1263)
                     /locus_tag="VF357_08855"
     CDS             complement(961..1263)
                     /locus_tag="VF357_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524299.1"
                     /translation="MRSLIAILCAACFLTFAQGAVTQALAAEPWETWPKKSDEVEADG
                     AAKAGEAGGAKTYAGMSAGTIGWIALGTAVVIGVAVAAGGGGGGGGSSTTPSHTTP"
     gene            complement(1316..>3343)
                     /locus_tag="VF357_08860"
     CDS             complement(1316..>3343)
                     /locus_tag="VF357_08860"
                     /inference="COORDINATES: protein motif:HMM:NF017857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HEX7524300.1"
                     /translation="PGAPARTRARPAGDEPFTGPNNFGVTGLFETPTARVMEENRYRL
                     GATLVRPYRYYFGTVGLFDGIEVNGRVTQVIGVPGFNSNNTSYGEFKDKAVDAKFRFL
                     KEGKYLPALAVAVSDPHGTRLYASQAIVASKQFFPFDFSLGFGNGRLGKQPLHSQGEG
                     FKIELFTDPRKWAREALPFGGIQYAATPWLSLLAEYSPIRYERQTTDPAQKKYFPTAV
                     RSPFNFGARVKPLKWLEIDASWQRGNEIGVTASVAFDIGRPLVPMYDPPYLETVESSR
                     APLQDRIAFALTDAGFSDIAVSDDGFTFRVDAQNDRYFFAPRAVEVLLATIAPFVPPN
                     VEYVRVQLKENGIPVAEAAVSASALSGAGSGFFPTDRIRAAAGFRSGNFDAPIRPTTH
                     RRWFDYSIKPSFEAFLNDPSGFLKYRAGLAGSLSAFPWRGGTALLGVEAYPVNNISTS
                     IAPLSIPIRSDIAKYKRETVTLGRLLFDQVFATREPVYFRAAAGLLETMFGGVDAETA
                     LPLWNGRILAGASGSVVRKRAPDDPFRFLGNNQYHTALLQGRLNVPEIDAAIDVKWGR
                     FLAGDRGVRVAASKFIRGVTLSAWYSATDTGVFTDPFNRGYHDKGISVEIPIRLFTGR
                     DSKTAYRYSLSPWTRDVAQDIDRYLPLFDRIGRNAGVLLDRDQVTIYRSIK"
BASE COUNT          544 a         1053 c         1191 g          555 t
ORIGIN      
        1 ttcacggggc tgcgcccggg cgagaagctg cacgaggagc tgatcgtcga gggcgaggac
       61 gtgagcggca cgacccaccc gaaggtgatg aagctgatcg gcaacgagcg gataccctcc
      121 tcctgggaaa agcggcttga ggagctgatc gcgtgcgccg tcaccgggga gcgttgtggt
      181 gtcgtcgaga agctggacgc cttggtgaaa gggtaccgcc cgaactacga attccacggc
      241 gtcgccgtcc ccgaacatgc tcgcgctgcc tctcccggcg cgacatcccc cgcaccaccc
      301 cccgaaggcg acctcccacc ggaccccgcc tccccctcca agtcggttca ctgagctccc
      361 cgcgacaccc ctcagcggga ccagaaggtt tcgacgaggc ccgctttcca gccgccggct
      421 tcacgaaaca ttttcagcct cgccgggtgg tccgccccat ggtgggtgag aaggatctcc
      481 gcgcggtctt tccccacgga gccgatctcc cagcggctgt gctccagggc ggcgtccggg
      541 tcgaaccggc gaaggaaggc gtcccagtag gcctgcgcga tcggacctcc ttccacgaaa
      601 tcgttgcgga ccgcatcgcg gcctggatcc ggcttcttca cggttgtggg attagtatcg
      661 gtgggggtcg gcttccccgg gcgcggctgc tttgcggcgg cggcaagggc ggacgacgtc
      721 tccgcgacga tcgtctcctg tgatttcacg ctgagcgcgt cgaagatagc ccggtaatcg
      781 tgggccttca tcgactggaa gagggactcg gcgcggtcaa ggatctcagg gacggcgggg
      841 ttttcttccg ggggtatggc gtacgcagga tgagctatga agaggaacgc gaacagaagg
      901 agcgaaacgc ccggggcgcg ccggggtgcg aggcaagcga cgcggagcga tatggcggcg
      961 tcagggtgtg gtatggctag gcgtagtgct gctcccgccc ccgcccccgc ctccgccggc
     1021 ggcgaccgcg acgccgatga cgacggcggt cccgagggcg atccatccga tggtccctgc
     1081 ggacatcccg gcgtaggtct tcgccccgcc tgcctcaccg gcctttgcgg ccccgtccgc
     1141 ttccacctcg tccgatttct tcggccaggt ttcccatggc tcggcggcaa gggcctgcgt
     1201 tacggcccct tgtgcgaagg tcagaaagca ggcggcgcac aggatcgcga tgagggatct
     1261 caacccggac ccaggatggt ttttgttcaa gggggcctcc atcggaaaca ccatgctatt
     1321 ttatagaacg atatatcgtt acctggtccc tgtcaaggag cactcccgcg ttgcggccga
     1381 tccggtcgaa gagaggcagg taacggtcga tgtcctgcgc gacgtcgcgc gtccacggcg
     1441 agaggctgta ccggtacgcc gtcttcgagt cccgcccggt gaagagacgg atggggatct
     1501 ccaccgagat ccccttgtcg tgatagccgc ggttgaacgg atccgtgaac accccggtgt
     1561 cggtggcgct gtaccaggcg gaaagggtca cgccgcggat gaacttcgag gccgcgaccc
     1621 gaacgccccg gtcgccggcg aggaagcgcc cccacttgac gtcgatcgcc gcgtcgattt
     1681 ccggcacgtt cagccgtccc tggagcagcg cagtgtggta ctggttgttg ccgaggaacc
     1741 ggaacgggtc gtcgggcgcc cgcttccgga cgacgcttcc cgatgcgccc gcgaggattc
     1801 tgccgttcca caacggaagc gcggtctcgg cgtcgacgcc gccgaacatc gtctccagca
     1861 accccgccgc cgcgcggaag tagaccggct cccgcgtcgc gaaaacctgg tcgaagagca
     1921 ggcgcccgag ggtgaccgtt tccctcttgt actttgcaat gtcgctgcgg atggggatgg
     1981 agagcggcgc gatcgaggtg gagatgttgt tcacggggta cgcctcgacg ccgaggagcg
     2041 cggtcccgcc gcgccacggg aacgcgctga gcgagccggc cagcccggcg cggtatttga
     2101 ggaagccgga agggtcgttg aggaacgcct cgaacgacgg cttgatgctg tagtcgaacc
     2161 agcggcggtg tgtggtcggg cggatcggtg cgtcgaagtt cccggagcgg aacccggcgg
     2221 cggcgcggat acggtcggtg gggaagaagc cgctgcccgc cccggagagc gcggaggccg
     2281 atacggccgc ctcggccacg gggatcccgt tctccttcaa ctgtacccgg acgtactcca
     2341 cgttcggcgg gacgaacggg gcgatcgtgg cgaggagcac ctcgacggcg cggggcgcga
     2401 agaagtaccg gtcgttctgc gcgtcgaccc gaaaggtgaa cccgtcgtcc gaaacggcga
     2461 tgtcgctgaa gcctgcgtcc gtgagggcga aggcgatccg gtcctggagc ggggcgcggg
     2521 acgattcgac ggtctccagg tacggagggt cgtacatcgg gaccagcggg cggccgatgt
     2581 cgaaggcgac ggaggcggtg acgccgatct cgtttccccg ctgccagctc gcgtcgattt
     2641 ccagccactt gagcggtttg acgcgggcgc cgaaattgaa cggagagcgg acggcggtcg
     2701 ggaaatattt cttctgcgcc gggtcggtcg tctggcgttc gtagcggatc gggctgtact
     2761 ccgcgagcag cgagagccac ggcgtcgcgg cgtactggat gccgccgaac gggagggcct
     2821 cgcgcgccca cttgcgcggg tcggtgaaca gctcgatctt gaacccttcc ccctgggagt
     2881 ggagcggctg tttgccgagg cggccgttgc cgaagccgag cgagaagtcg aacgggaaga
     2941 attgcttgct ggcgacgatc gcctgggagg cgtaaaggcg cgtgccgtgc gggtcggaga
     3001 ccgccactgc gagggcgggg aggtacttcc cctctttgag gaaccggaac ttcgcgtcca
     3061 cggccttgtc cttgaattcc ccgtaggaag tgttgttgct gttgaaaccc ggcacgccga
     3121 tcacttgggt gacccggccg ttcacctcga tcccgtcgaa caggccgacg gtgccgaagt
     3181 aataccggta cgggcggacc agcgtggcgc cgaggcggta ccggttctcc tccatgacgc
     3241 gtgcggtggg ggtttcgaaa aggccggtca cgccgaagtt gttcgggccg gtgaacggct
     3301 cgtcgccggc ggggcgggcg cgcgtcctgg ccggggcgcc cgg
//
LOCUS       DASVJL010000423         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1759736, whole genome shotgun sequence.
ACCESSION   DASVJL010000423 DASVJL010000000
VERSION     DASVJL010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759736"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /gene="metK"
                     /locus_tag="VF357_08865"
     CDS             complement(<1..956)
                     /gene="metK"
                     /locus_tag="VF357_08865"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX7524301.1"
                     /translation="MSEFLFTSESVTGGHPDKVADQISDAVLDEILRQDPRGRVACET
                     MVTTGLVVVAGEITTKAIVDFPDVVRKAVTDIGYTGTSKGFDAHNCAVVVAIDRQSAD
                     IAQGVDRGDEEKQGAGDQGMMFGFACDETKELMPMPISFAHKICARLTEAREKKVLSW
                     LRPDGKSQVTVRYVDGVPREVTAVVCSTQHAEEVGRKALTEGVLEEVVRKAVPRDLLS
                     RKVKFYINPTGRFVVGGPHGDTGLTGRKIIVDTYGGYSRHGGGAFSGKDPSKVDRSAA
                     YMARYVAKNVVAAGLACKCEVQLAYAIGVAEPVSVLVETFGTG"
     gene            complement(1006..>2295)
                     /gene="ptsP"
                     /locus_tag="VF357_08870"
     CDS             complement(1006..>2295)
                     /gene="ptsP"
                     /locus_tag="VF357_08870"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX7524302.1"
                     /translation="AGIRDPVIIVAHDLSPADTAQILKSPVLGFATDVGSRTSHTAIT
                     ARSLGIPAVVGVEGATEEYRSAETVIVDGEEGVVVFDPTEETVREYQGRRKAYAQRTR
                     DLAKFARLPTVTRDGKTLLLLANIEFPEEADVALRSGAYGVGLYRSEFLFLNRKDLPS
                     EEEHFETYRKVAEKFVRHPVTIRTLDLGGDKFASQLELAEEMNPAMGLRAIRFCLKEK
                     EIFKPQLRAIMRASTYGKVRMMFPMISGVGELREALAVVEEVRGELRRRRIPYDKEMP
                     IGIMIEIPAAAIVADLLAREVGFFSIGTNDLIQYSLAIDRVNEHVSYLYEPLHPAILR
                     LIRRVVEAGHDAGIPVSMCGEMAGEPIYSYALLGLGLDELSMNAAAIPRVKRILRKSV
                     AYEAKEFAGSLLLHATADEIGRVLRKKVEDLFPDERF"
BASE COUNT          376 a          836 c          682 g          401 t
ORIGIN      
        1 cccgtgccga aggtttcaac gaggacggac accggctcgg cgacgccgat ggcgtaggcg
       61 agctgcactt cgcacttgca cgcaagaccc gcggcgacca cgttcttggc cacgtaccgg
      121 gccatgtacg ccgcgctgcg atcgaccttg gaaggatcct tcccggagaa cgccccgccg
      181 ccgtgccggc tgtacccccc gtaggtgtcg acgatgatct tccgtccggt gagccccgtg
      241 tcgccgtgag ggcccccgac gacgaaccga ccggtcgggt tgatatagaa cttcaccttc
      301 ctggacaaca ggtctctcgg caccgctttc cggacgacct cctccagcac cccctcggtg
      361 agggccttgc ggcccacctc ctcggcgtgc tgggtggaac agaccacggc ggtcacctcg
      421 cgcgggacgc cgtccacgta gcgcaccgtc acctggctct tcccgtcggg ccgcagccac
      481 gaaagcactt tcttctcccg cgcctccgtg agccgcgcgc aaatcttgtg ggcaaaggag
      541 atcggcatcg gcatcagttc cttcgtctcg tcgcaggcga acccgaacat catcccctgg
      601 tccccggcgc cctgcttctc ctcgtcgccc cggtcgaccc cctgcgcgat gtcggcggac
      661 tggcggtcga tcgcaacgac cacggcgcag ttgtgggcat caaacccctt cgaggtcccc
      721 gtgtagccga tatcggtgac cgccttgcgg acgacgtccg gaaaatcgac gatcgccttc
      781 gtggtgatct ccccggccac cacgaccagc ccggtagtca ccatcgtctc gcacgccacc
      841 cgtccgcggg gatcctgccg gaggatttcg tccagcaccg cgtcggagat ctggtccgcc
      901 accttgtcgg ggtgcccgcc cgtcaccgat tcggacgtaa aaaggaattc gctcatgccc
      961 acctctcctg caccgcgaaa tggattcgaa atactttagt atatcctaaa aacgctcgtc
     1021 ggggaacagg tcctcgactt ttttgcgaag cacccggccg atctcgtccg cggtcgcgtg
     1081 cagcagcagc gaacccgcga actccttggc ctcgtacgcc accgacttgc ggaggatccg
     1141 cttcacccgc gggatcgcgg cggcgttcat gctgagctcg tccagcccga ggccaagcag
     1201 cgcataggag tagatcggct cgccggccat ctcgccgcac atcgacaccg ggatccccgc
     1261 gtcgtgcccc gcctccacga cccgccggat cagccgaagg atcgcggggt gaagcggctc
     1321 gtaaagatag gagacgtgct cgttgacccg gtcgatcgcc agcgagtact ggatcaggtc
     1381 gttcgtgccg atgctgaaga atccgacctc ccgcgcgagc aagtcggcca cgatcgccgc
     1441 ggcggggatc tcgatcatga tcccgatggg catctccttg tcgtagggga tccgccggcg
     1501 acgaagctct ccccgcacct cttcgaccac ggccaacgcc tcccgaagct ccccgacccc
     1561 cgagatcatc gggaacatca tccgcacctt cccgtacgtc gaggcgcgca tgatcgcacg
     1621 gagctggggc ttgaagatct ccttttcctt caggcagaac cggatcgccc gcagccccat
     1681 cgccgggttc atctcttcgg cgagctcgag ctgggacgcg aacttgtcgc cgccgaggtc
     1741 caaggtgcgg atcgtgaccg ggtggcgcac gaatttctcc gcaaccttcc ggtacgtctc
     1801 gaagtgctcc tcctcggaag ggagatcctt ccggttgagg aagaggaact ccgagcgata
     1861 gagccccacc ccgtaggcgc cgctgcgcag cgcgacgtcg gcctcctcgg gaaactcgat
     1921 gttcgccagt agcagcagcg tcttcccgtc tcgcgttacc gtcggcagcc gcgcgaactt
     1981 cgcaaggtcg cgggtccgct gcgcgtaggc cttccgccgc ccctggtact cccggaccgt
     2041 ctcctccgtc gggtcgaaga cgaccacccc ttcctctccg tcgacgatca ccgtctccgc
     2101 ggacctgtat tcctccgtcg ccccctcgac cccgacgacc gccgggatcc ccagagagcg
     2161 cgccgtgatc gcggtgtggg acgtccggct tcccacgtcg gtggcgaacc cgagcacggg
     2221 gctcttcagg atctgcgcgg tgtccgccgg ggagagatcg tgggccacga tgatcaccgg
     2281 gtcacggatc ccggc
//
LOCUS       DASVJL010000424         7177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1290451, whole genome shotgun sequence.
ACCESSION   DASVJL010000424 DASVJL010000000
VERSION     DASVJL010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290451"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF357_08875"
     CDS             <1..213
                     /locus_tag="VF357_08875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7524303.1"
                     /translation="VDHGDGFITKYGHTRANLVDTGAQVGPDTIIAEVGNTGQSTGPH
                     LHFEVAYEGKNVRPETVLAGIAKGNG"
     gene            258..542
                     /gene="flgM"
                     /locus_tag="VF357_08880"
     CDS             258..542
                     /gene="flgM"
                     /locus_tag="VF357_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEX7524304.1"
                     /translation="MKINSHKPPERVDVVRLQKPGEKNGSARLSGKPDSTDKVNLSGN
                     AKELGDIMSAINRLPDVRAEKVAGIRGQIDSGKYVVDPGEIARKMVDEIV"
     gene            539..1000
                     /locus_tag="VF357_08885"
     CDS             539..1000
                     /locus_tag="VF357_08885"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HEX7524305.1"
                     /translation="MKSLGALKIILVEQEKCCKALVDLLQKERAYLIDFEVGGIEELT
                     KGKDTLLLRLRLLEEERLRLTAVFAAGPGGPPADVTLRGLVDRTGDAELAGIRLKLVS
                     LAQSIEDLNSFNRHLIDRSLGSIRCATGFFQAFGGSRPERSESGALVSRET"
     gene            1002..2633
                     /gene="flgK"
                     /locus_tag="VF357_08890"
     CDS             1002..2633
                     /gene="flgK"
                     /locus_tag="VF357_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEX7524306.1"
                     /translation="MSLINLLNIGKSAIFANQAAINVTGNNIANVNTPGYTRQAAIFN
                     IATPIVPVAGGYIGMGVNVTSIARRYDQFISGQLLAQEQNSGKSSTMDQILGQVEQVF
                     NGNAGAGLSSSLTAFFDAWQSLSANPGDTAQRTVLLSDANNLVTTAKQMESSILGTLK
                     DANKAISDAAGQVNSIATDIARLNAQIVQIESGGGGAKANDLRDARDQLVTQLSGLVD
                     IGTLEDKNGSLTVTVGMRNLVGGETVNLMTAISNAAGDAGLSLDGVDVTSRITKGSVG
                     GLLASRDAVTSGPLADLRRLAAAVTNETNQIHSAGVGLDGTTGNDFFTPLSLSSTGSS
                     AGASVTSATITDPSALTLSDYSISIGAGNAYSVTNKDSGVVVATGTYASGNPIAFDGI
                     SVVLTGTAAAGDAFTVSPLTGAISNFGVAVSDPRKVAAASAASALPGDNTNALRIAGL
                     ADAAVSSLGGATFSGYYGGLVSDVGRLAKNASDLSQFDSNLQSELQNRRDSASGVSLD
                     EEATNLIAYQRAYEAGARIIKITDELFQTVLQMFP"
     gene            2630..3634
                     /gene="flgL"
                     /locus_tag="VF357_08895"
     CDS             2630..3634
                     /gene="flgL"
                     /locus_tag="VF357_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEX7524307.1"
                     /translation="MRVSTSQVYRQTTDSLMRNLAEIMRLNTQVASGKKINKPSDDPA
                     GAGRALAYKVAIDAGDQYAKNIAVASNTIGFAENTLGSVSSALVRAKELALQGANGTE
                     DAASRDAISKEASQLRDQLLSLANSRFGNEYVFSGFKTDVPAFDSSFVYQGDSGSINV
                     TVDQGTMIPRNVTGSEAFGYSLSAPETVTTDGGKIVHYIPGSGTTVNVEIRAADDTTV
                     LDSFHFDNAMQMTDLLSQALVNNDAARVSALMKPLDDAMGHVNDVRADLGARLNRLDD
                     QGKRLEDTKFTTQTALSGVEDADIASTVSGIAKANVALQALQASSAKILSQSLLDFLK
                     "
     gene            3729..3953
                     /gene="csrA"
                     /locus_tag="VF357_08900"
     CDS             3729..3953
                     /gene="csrA"
                     /locus_tag="VF357_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEX7524308.1"
                     /translation="MLVLTRKSEEVVKIGDEITVTVLEVRGNQVRIGISAPPGVRIYR
                     GEIYEKIKSENLVSSGLSVDDFASIKEKVR"
     gene            3954..4340
                     /gene="fliW"
                     /locus_tag="VF357_08905"
     CDS             3954..4340
                     /gene="fliW"
                     /locus_tag="VF357_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HEX7524309.1"
                     /translation="MAAFNTTRFGPVEVSEDRVIRFPVGLLGFPHLLRYVLIDYKDTP
                     LRWLQAVDDPDVAFIVADPKSIAGDWTVALGGDVVQFLQIEHEDDLAVLLILRVEGGK
                     VIANLNGPLAINSSRMLGVQAVHDGA"
     gene            4390..5463
                     /locus_tag="VF357_08910"
     CDS             4390..5463
                     /locus_tag="VF357_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX7524310.1"
                     /translation="MKIDAVAQIGNSLTFLSPSGDGMRYAVGDVVQAEVLSILPGGSV
                     SIRITTESGKSGIVAARSEVPLAEGESVLLKVVGGEREVALRFLGVIREGGTPGGMPA
                     GLPEMYRELASELAASRLSIADARQAQESFLLLPEVVKGSVPGFEAIGRSPGMEGLDG
                     TVLRETVEGSGILLETKLKLAAKELTPGADRKEALLRLGEALRDRDVAGAVSKAGGSP
                     GEAGVRADGLVSTIESFQLASAAQGGLYVPLSLQWDELADGEMLFRKRIRGKGESYTC
                     EINLDLRPLGKMSASVTMYDGAFFVSLLPQSEATRSLMASRSAEVERRFREAGLALRA
                     VSITRKNSVDFGIPAPDGVDVEV"
     gene            5466..5765
                     /locus_tag="VF357_08915"
     CDS             5466..5765
                     /locus_tag="VF357_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX7524311.1"
                     /translation="MAKRNRQAAALRYVQGKDASPRLMAKGKGAVADRILEIARRNGI
                     PIREDRELVQVLASLDLYQEIPPELYKAVAEILVFLYSLNQGAIQALPMETHRGG"
     gene            5779..7071
                     /locus_tag="VF357_08920"
     CDS             5779..7071
                     /locus_tag="VF357_08920"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX7524312.1"
                     /translation="MAKYTVFAYGSRKAFPVEGLSIGTRLPFDVFVNEGNVFRKLYDA
                     GQLIGPEERAALRRTGATEAYVDAKDAPVLGHYLQRTSCENPADPGDPEVCREFFEHK
                     RRYYQVDRTFLLPGSRVEFGIYILGDLRLTPLLEAMEGEPGIIPGGISAAAGDVVIRN
                     EDIPLYQRYLEALLDGGAESVSAPAEREKIRAVAIKEKSKAIIKALIEDPRSGENINM
                     AVGLATQLTDCILSSREVIHELISLDSYDMYTYTHSVNVAVLSVAIGVTHGMERHDAE
                     SLGVGALLHDIGKSGIPAEILNKFGKLSDPEYEIVKGHVVEGARILRAHEAIPRSASE
                     VVLQHHERLSGLGYPNGLSGSAINLFGRICAIADCYDAMTTRRVYQRGYTPYEALFQI
                     TKEVENYDRDVIESFIRMLGKVGVPEKPHAEEVSLPQG"
BASE COUNT         1410 a         2293 c         2318 g         1156 t
ORIGIN      
        1 gtcgaccatg gtgacggttt cattacgaaa tacgggcaca cccgggccaa cctcgtcgac
       61 acgggagccc aggtcggtcc ggatacgatc atcgccgagg tggggaacac ggggcaatcg
      121 accggtcccc acctccactt cgaggttgcg tacgagggaa aaaatgttcg ccctgagacg
      181 gttttggccg gaatcgctaa aggaaacggc tgatcaaccg ataagtatga taaaggaacg
      241 tctcatgggg gatatgcatg aaaatcaaca gccacaaacc gccagagagg gtggacgtcg
      301 tccgtctgca gaagccgggg gagaaaaacg ggtccgcccg gctgtcaggg aagccggact
      361 ccacggacaa ggtcaacctg tccggcaatg cgaaggaact tggtgacatc atgagcgcca
      421 tcaaccgact ccccgacgtc agggcggaga aggtggccgg gatccggggt caaatcgact
      481 ccgggaaata cgtcgttgac cccggggaaa tcgcgcggaa gatggtcgac gaaatcgtat
      541 gaagtccctg ggcgccctca agatcatcct cgtggagcag gagaagtgct gcaaggcact
      601 cgtcgacctg ctccagaagg agagggcgta cctgatcgac ttcgaggtgg gcgggatcga
      661 ggagctcacg aaggggaaag acaccctcct cctgcggctg cgcctcctcg aggaggagcg
      721 gctccgcttg accgcggtgt tcgccgccgg gcccggcggc ccacccgccg acgtcacgct
      781 ccggggcctc gttgaccgca cgggcgacgc cgagctcgcg ggaatccgcc tcaagctcgt
      841 ttcactcgcc cagagcatcg aggacctgaa cagcttcaac aggcacctca tcgaccgttc
      901 ccttggcagc atccgttgcg ctaccggctt cttccaggcg ttcgggggtt cccgccccga
      961 gcgatctgaa agcggggcgc tcgtatcgcg ggagacgtga gatgtcgctc ataaacctct
     1021 tgaacatcgg aaaatccgcg atcttcgcga accaggcggc catcaacgtc acggggaaca
     1081 acatcgccaa cgtcaacacc cccggctaca cccgccaggc ggcgattttc aatatcgcga
     1141 cccccatcgt gccggtggcc ggcgggtata tcggcatggg cgtcaacgtt acgagtatcg
     1201 ccagacgcta cgaccagttc atctcgggcc agctcctcgc gcaggaacag aactccggca
     1261 aatcgtcgac gatggaccag atcctcggcc aggtcgagca ggtgttcaac ggcaacgcgg
     1321 gtgcgggcct ctcaagctca ctcaccgcgt tcttcgacgc atggcagagc ctttcggcaa
     1381 accccgggga cacggcgcag cggaccgtcc tgctctcgga cgcaaacaac ctcgtcacca
     1441 ccgcgaaaca gatggagagc agcatcctcg ggacgctcaa ggatgccaac aaggcgatat
     1501 ccgacgcagc cggccaggtc aacagcatcg cgacggacat cgcccggctc aatgcccaga
     1561 tcgtgcagat cgagtccggt ggcgggggcg cgaaggcgaa cgaccttcgg gacgcgcgcg
     1621 accagctcgt cacccagctc tccggcctgg tggacatcgg cacgctggag gacaagaacg
     1681 gctccctgac cgtcaccgtg gggatgcgca acctcgtcgg cggcgaaacg gtcaacttga
     1741 tgacggccat cagtaacgcg gccggggacg cgggtctctc cctcgatggg gtggacgtca
     1801 cctcgcggat caccaaaggg agcgtaggcg ggctccttgc atcgagggat gccgtcactt
     1861 cgggcccgct cgccgacctg cgccgcctcg ccgccgccgt cacgaacgag acgaaccaga
     1921 tccacagcgc cggcgtcggg ctcgacggga cgacgggaaa cgacttcttc acgccgctgt
     1981 ccctgtcctc gaccgggtcc tccgcgggag cctccgtaac gtccgccacg atcaccgacc
     2041 cgtccgcact cacgctgtcg gactactcca tctccatcgg tgcgggaaac gcttacagcg
     2101 tgacgaacaa ggacagcggc gtggtcgtcg ccaccgggac gtacgcatcc gggaacccga
     2161 tcgcgttcga cggcatcagc gtcgtcctga cggggacggc ggctgcgggc gacgcgttta
     2221 ccgtgagccc cctgaccggg gccatatcga acttcggcgt cgcggtgtcc gatccccgga
     2281 aggtcgccgc tgcgtccgcc gcgtccgccc tgcctggaga caatacgaac gcgctccgga
     2341 tcgccgggct cgccgatgcc gccgtttcct ccctcggagg ggcgacgttc tccgggtatt
     2401 acggcggact cgtttcggac gtcgggaggc ttgcgaagaa cgcttcggac ctgtcgcagt
     2461 tcgactcgaa cctgcagtcg gagcttcaaa accggcggga ttccgcctcc ggggtgtcgc
     2521 tcgacgagga ggcgacgaac ctcatcgcct accaacgtgc atacgaggcg ggtgcgcgga
     2581 tcatcaagat tacggacgaa cttttccaga ccgtgctgca gatgtttcca tgagagtctc
     2641 cacttcccag gtctatcgcc agaccacgga ttccctgatg cggaatctcg ccgagatcat
     2701 gcgtctcaac acgcaggtgg cctcgggcaa gaagatcaac aagccttccg acgatccagc
     2761 gggggcgggg cgggcgctgg cctacaaggt cgcgatcgac gccggcgatc agtacgcgaa
     2821 aaacatcgcc gtcgcctcca acacgatcgg gtttgccgaa aacaccctcg gctccgtctc
     2881 ctccgctctc gtgcgggcga aggaactcgc gctccagggc gcaaacggaa ccgaggacgc
     2941 cgcatcgcgg gacgcgatct ccaaggaggc ctcccagctc cgggaccagc tcctttcgct
     3001 cgccaacagc cgcttcggaa acgaatacgt cttctccggc ttcaagacgg acgtcccggc
     3061 cttcgattct tccttcgtat atcagggcga ctccgggtcg atcaacgtga cggtcgacca
     3121 gggcacgatg atcccgcgca acgtcacggg ttccgaagca ttcggctact ccttgtcggc
     3181 gccggagacc gtcacgaccg acggcggaaa gatcgtccac tacatccccg ggagcggtac
     3241 gaccgtcaac gtcgagatcc gcgcggccga cgacacgacc gtcctcgaca gcttccattt
     3301 cgacaacgcg atgcagatga cggatctcct gtcgcaggcg ctcgtaaaca acgacgcggc
     3361 gagggtctcc gccctgatga agccgctcga tgacgcgatg gggcacgtga acgatgtccg
     3421 ggccgacctc ggcgccaggc tcaaccggct ggacgaccag gggaagcggc tcgaggacac
     3481 gaagtttacg acgcagaccg cgctgtccgg cgtggaggat gccgacatcg cctcgaccgt
     3541 gagcggcatc gcgaaagcca acgtcgcgct ccaggcgctc caggcatcct cggcgaagat
     3601 cctgtcgcaa tccctgctcg atttcctcaa gtaggaagcg cgctataatc atgggacaag
     3661 ggggaggatg acaccggaac caattttatc ggcgccggaa tgaccacccg aacgggatcg
     3721 gaggcaggat gctggttctg acacggaagt cggaagaggt ggtaaagatc ggcgacgaga
     3781 tcacggtgac ggtgctcgag gtccggggaa accaggtccg tatcggcatc agcgcacctc
     3841 ccggagtaag gatctaccgg ggggagatct acgagaagat caagtcggag aacctcgtgt
     3901 cgtccggtct ctcggtggac gatttcgcga gcatcaagga gaaggttcgc tgaatggctg
     3961 ctttcaacac gacccgtttc ggaccggttg aggtgagcga ggaccgggtc atccggtttc
     4021 ctgtcgggct cctcgggttc ccccacctcc tccggtacgt cctcatcgac tacaaggaca
     4081 ccccgctgag gtggctccag gccgtcgacg atccggatgt cgcattcatc gtggcggacc
     4141 cgaagtcgat cgccggcgac tggaccgtcg cgctaggcgg cgacgtcgtc caattcctcc
     4201 agatcgagca cgaggacgac ctggcggtcc tcctcattct gcgggtggag ggcggcaagg
     4261 tcatcgccaa cctcaacggt cccctcgcga tcaattccag ccggatgctg ggagtgcagg
     4321 ccgtccacga cggggcatga agcccttact ttcccctccc ggcgttttct cccctccccg
     4381 ggcttcttca tgaaaatcga cgcggtcgca cagatcggaa attcgctgac cttcctgtcg
     4441 ccctcgggcg acgggatgcg ctacgcggtc ggcgacgtgg ttcaggccga agtcctttcg
     4501 atcctccctg gcgggagcgt ctcgatccgc atcaccacgg aatcgggaaa gagcgggatc
     4561 gtcgcagccc ggtcggaagt cccgctggcc gaaggggaga gcgttctcct caaagtggtg
     4621 gggggagagc gcgaggtcgc cctccggttc ctgggggtga tccgcgaggg gggcacgccg
     4681 ggaggcatgc ccgccggcct ccccgaaatg taccgggagc ttgcatcgga gctggccgcc
     4741 tcccgcctgt cgatcgcgga cgcgcgccag gcgcaggagt cgtttctcct ccttcccgag
     4801 gtcgtaaagg ggtccgtgcc ggggttcgag gcgatcgggc gatcgccggg aatggaaggg
     4861 ctggacggga cggtcctcag agagaccgtc gaggggtccg gcatcctcct tgaaacgaag
     4921 ctcaagctcg ccgcgaagga gctcaccccg ggggcggacc ggaaggaggc gctccttcgt
     4981 ctgggcgagg cgctgcggga cagggacgtg gcgggggcgg tcagtaaggc cggaggaagc
     5041 cccggggaag cgggcgtgag ggccgacggc ctcgtgtcca ccatcgagtc gtttcagctc
     5101 gcctccgcgg cccagggggg cctctacgtt ccgctttccc tccaatggga cgagctcgcc
     5161 gacggagaga tgctcttccg gaagaggata cgggggaagg gcgaatccta cacgtgcgaa
     5221 atcaacctcg acctgcgtcc cctcgggaag atgtccgctt ccgtcacgat gtacgacggc
     5281 gcttttttcg tctccttgct gccgcagagc gaggccacgc gttccctgat ggcttcccgc
     5341 tccgcggagg tcgaacggcg gttccgggag gcggggctcg cactgagggc ggtcagtatc
     5401 acccggaaga acagtgtgga tttcggaatc cccgcgccgg acggtgtcga cgtggaggtg
     5461 taggggtggc aaagaggaac cggcaggccg cggcgctcag gtacgttcag gggaaggacg
     5521 cttctccccg cctcatggcc aagggaaagg gggcggttgc ggacaggatc ctcgagatcg
     5581 cgaggcgcaa cggaatcccg atccgggagg accgggagct cgtccaggtt ctcgcttcgc
     5641 tcgaccttta ccaggagatc ccgcccgaac tctacaaagc cgtcgccgag atcctggtgt
     5701 ttctctattc actcaaccag ggggcgattc aggcgctgcc gatggaaacg caccggggcg
     5761 gctgaggaga gcggtcggat ggcgaagtac acggtattcg catacggatc ccgcaaggcg
     5821 ttcccggtcg aggggctctc catcggcacg cgcctgccgt tcgatgtttt cgtcaacgaa
     5881 gggaacgttt tccgcaagct gtacgacgcg gggcagctca tcggcccgga ggagagagcc
     5941 gcgctccgcc ggacgggcgc caccgaggcg tacgtcgacg ccaaggatgc acccgtgctc
     6001 ggtcattatc tccagaggac gtcctgtgaa aacccggcgg accccggcga cccggaggtg
     6061 tgccgggagt tcttcgagca caagaggcgg tattaccagg tggaccggac atttctcctc
     6121 cccggttccc gggtcgaatt cgggatctac attctcggcg acctgcggct gacccccctc
     6181 ctggaggcca tggaagggga acccggcatc atccccggag gcatctccgc cgccgcaggc
     6241 gacgtcgtca tccggaacga ggacatcccg ctctaccagc ggtacctgga ggccctgctc
     6301 gacggaggcg cggaatccgt ctccgccccg gcggagcggg agaagatccg cgccgtcgcc
     6361 atcaaggaaa aatcgaaagc catcatcaag gccttgatcg aggacccccg aagcggcgag
     6421 aacataaaca tggcagtggg gctggccacg cagctcaccg actgcatcct gagcagccgg
     6481 gaggtcattc acgaactcat ctccctggac agctacgaca tgtacactta cacccactcc
     6541 gtgaacgtcg ccgtcttgtc cgtcgcgatc ggggtcaccc acggcatgga gcggcacgac
     6601 gcggagagtc tcggcgtcgg agcccttctg cacgacatcg gcaagagcgg gatcccggcg
     6661 gaaatactta acaaattcgg taaactcagc gacccggagt acgagatcgt gaaggggcac
     6721 gtcgtggagg gggcgaggat cctgcgggcg cacgaggcga ttccgcggag cgcctccgag
     6781 gtcgtcctgc agcaccacga gcggctctcc ggcctcgggt accctaatgg actgtccggg
     6841 agcgccatca acctgttcgg gcggatctgc gcgatcgccg actgctacga cgcgatgacg
     6901 acccgccgcg tgtaccaacg gggctatacg ccctacgagg ccctcttcca gatcacgaag
     6961 gaggtcgaaa actacgaccg cgacgtcatc gagtccttca tccggatgct cgggaaggtc
     7021 ggggtgccgg aaaaacccca cgccgaggag gtttctcttc cacagggcta aagtatttcg
     7081 gggaacggcc gattaatata cctgaggccg aacgaggcaa cgctggagag gagaggaaaa
     7141 cggaagaacg ccgggcagga acgccggcaa tacagcc
//
LOCUS       DASVJL010000425         5353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_165679, whole genome shotgun sequence.
ACCESSION   DASVJL010000425 DASVJL010000000
VERSION     DASVJL010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165679"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1615
                     /locus_tag="VF357_08925"
     CDS             230..1615
                     /locus_tag="VF357_08925"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7524313.1"
                     /translation="MIEGDPGARLSVTISNGMTVEVRLGPKEVKIGRGHEADLQLPDR
                     SVSRLHAKVFRVGQQYFLADLRSRNGTHADGKRITQLALEDGRMFQVGPFRIHFHRPV
                     SGFPAGEEPTVSPGTATAITDSFEPEPVRETKRVDAHAVPDQAPFGLIGGSAHVRKLV
                     ATIRRVAASDVPVLIEGETGSGKELVARGIHDASARRERPFIVVNCGAISPELIESEL
                     FGHEKGAFTGATALRKGAFELANSGTIFLDEIGELPLALQPKLLRVLEQKEVKRVGGN
                     DLLLADVRILAATNRNLREEIARKMFREDLYFRIGAITVPIPPLRDRREDVTPIARHF
                     LSGMKSPASGHLPGLSPAALDSLISHDWPGNVRELRNAIQRAVVMTENGELTGPDFSF
                     LRQAAKPGAEAESPSGLSRWEQAERTNILGELARQMGNKTKTARELGIAKSTLFEKLK
                     RYGIRTAEFDR"
     gene            1792..2037
                     /locus_tag="VF357_08930"
     CDS             1792..2037
                     /locus_tag="VF357_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524314.1"
                     /translation="MRRALGKKRNGQGLTEYIIIVALVAVAGIGIVNIFGNQLRNQFH
                     TIVKAMSGSETATVQSLAGKAGTEANQKNLSTYAGSK"
     gene            2051..2494
                     /locus_tag="VF357_08935"
     CDS             2051..2494
                     /locus_tag="VF357_08935"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX7524315.1"
                     /translation="MIEFLLAGVPLLLLMLAIVQLSLLWAGKSAVDTAAHLAARKFAR
                     IAREDFRKAREMAFLEAFQVCRNRPGGSFGSAAMTALDVTKDGEQGANRADAGEALCI
                     RLTHGVELVVPWIDRVLFTLSPGKKLRLGEHYYLMMQSTRWVTVE"
     gene            2491..3408
                     /locus_tag="VF357_08940"
     CDS             2491..3408
                     /locus_tag="VF357_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524316.1"
                     /translation="MTMIGYFSRQAVQLRAGNRGQATALFLVVAATLLVVVFASIRLH
                     HVAVARVSTADSVDAIALSAATWEARCLNLIAALNDGAIQCFRVIRWTCVLWAALAVA
                     AAFGFGVPAFAMYTREARRLISGYWDTAHLMVSWSEKIRKAAPYLVLGEVAALSKQRN
                     VAGILFPLNPAGPHDGKNTLELHLAPGPPIFLLDAIAPISGVLNQLKRIPFLNSAMKT
                     VIALLDTALRGIVGTRKGPIRMLEPEIDFPDRQFVRFEGIHTVPDLPIPFLDERGKRR
                     VIEDATAQPYGGGPAEMTWRSRLAERGKK"
     gene            3405..3881
                     /locus_tag="VF357_08945"
     CDS             3405..3881
                     /locus_tag="VF357_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524317.1"
                     /translation="MKGRRKSPRGQSLVETVLVVPLLLILLGGGYWFYRDLSLSSSAE
                     SAAHAQMLRAGRRLSGIESRLSGSIHPGDDVARIEAHNDPLIGEVPLFRGLAGRTIAS
                     ANVSLGKEPVGAFLDLPSHALRREAEGAVDCWGKETPSGATVRRTVQGILLTGVLR"
     gene            3878..4444
                     /locus_tag="VF357_08950"
     CDS             3878..4444
                     /locus_tag="VF357_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524318.1"
                     /translation="MNRKILAGLAGAATLFVSLCTVDTGNARRKEISSTRRFVIDGRS
                     FDSDESSPDNGSLLKRELGRMGVRLPDGFPLPEESVLSHPAFEGRLKESRSPVLEAPR
                     LPAGLTAEHTLRMEGEGMPVDLVFGKLGAPGPSIRSRLLLSGWKSVSTANGSGETHVL
                     QIRHGKETSIVCLDDAEGTFLLFREVGR"
     gene            4350..5198
                     /gene="cpaB"
                     /locus_tag="VF357_08955"
     CDS             4350..5198
                     /gene="cpaB"
                     /locus_tag="VF357_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX7524319.1"
                     /translation="MCCKSVMERKPALFASMMRKARSFSSGKSAGKVRTLLPLFAGLL
                     LCGFALAAAGRRVATVEKDILRQANPVEVVVASVPIPAGETFGARNLAKKAIPSSGTG
                     QRNVPATDFELLVGARAKTAIDPGEPVLWTDVEEPYDTDAFSRIVLPGRRAMTLGVDT
                     TSSFAGLVHPGDRVDLLVERTGANSADWVRDIPVIAVDRDHNRLAHPTDKEEATTVTL
                     MVSPAEGNRIARASGKVHWFLRNPDDNAAVASLRPAKPMTPHVVEVWKGGVKIPPNFA
                     AKENHE"
BASE COUNT          939 a         1750 c         1746 g          918 t
ORIGIN      
        1 acgatcccga gaccgccctc gtcttccgga ggcggcggtg gcggcggatg cctttccatc
       61 acccgatccg gcggcgggat gtcgatcggc acgtcgctct tttccgtcgt gctcctcctc
      121 cttcccgcac tcgctctcgg attgcggcgt ttttcccgtc gacgggagcg agacgtcccg
      181 ttccggcacc ccctttgcta agaaaagggg aaacggggtg gagggggcga tgatcgaggg
      241 cgatccgggc gcgaggcttt ccgttaccat ttcgaacggg atgacggtcg aggtgcggct
      301 ggggccgaaa gaggtcaaga tcggcagggg gcacgaggcc gacctccagt tgccggaccg
      361 atccgtttcg cggctccatg ccaaggtctt ccgcgttgga cagcaatatt tcctcgcgga
      421 ccttcgcagc cgcaacggca cgcacgccga cggcaagcgg atcacccagc tggccctgga
      481 agacgggagg atgttccagg tgggcccgtt ccggatccac ttccatcgcc ccgtgtccgg
      541 attccccgcg ggggaggagc cgacggtctc tccagggacg gcgaccgcca tcaccgattc
      601 cttcgaaccg gaaccggttc gcgaaacgaa gcgggtggat gcgcacgcgg tccccgacca
      661 ggcgccgttc ggcctgatcg gcggatcggc ccatgtacgg aaactggtcg cgacgatccg
      721 ccgcgtcgcc gcgtccgacg tgccggtcct gatcgagggg gaaaccggga gcggcaagga
      781 actggtcgca cgggggatcc acgacgcttc cgcgcgccgg gagcgtccgt tcattgtcgt
      841 caactgcggg gcgatctcgc ccgagctgat cgaaagcgag ttgttcggac acgagaaggg
      901 ggcgttcacc ggggccaccg cgctacggaa aggagcgttc gaactcgcca acagcggtac
      961 gatattcctg gatgaaattg gggagttacc tcttgcactt caaccaaaac ttttacgtgt
     1021 ccttgagcag aaagaggtca agcgtgtcgg agggaacgac ttgttgctgg ccgacgtgcg
     1081 gatcctcgcc gccacgaacc ggaacctccg ggaggagatc gcccgcaaga tgttccgcga
     1141 agatctctat tttcggatcg gcgccatcac ggtgccgatc cctcccctgc gggaccggcg
     1201 ggaggacgtg accccgatcg cccggcactt cctttccggg atgaagagtc ccgcctcggg
     1261 gcatcttccc ggactctccc ccgccgcgct ggactccctg atctcccacg actggcccgg
     1321 gaacgtgcgc gagctgcgca acgcgatcca gcgggccgtg gtgatgacgg aaaacgggga
     1381 gctgaccgga ccggatttct ccttcctgcg ccaggcggcg aagccgggcg cggaagcgga
     1441 gagcccgtcc gggctctcgc gctgggagca ggcggagcgg acgaacatcc tcggcgaact
     1501 cgcccgccag atggggaaca agacgaagac ggcgcgcgag ctcgggatcg ccaagtcgac
     1561 cctgttcgag aagctgaaga gatacgggat ccgcaccgcg gagttcgacc ggtaaccgca
     1621 cttatcgttc gaaattcgaa ccgtttcctc cccatattcc ttccaccgag ggtacccttc
     1681 ctgtaaccct ccgatccatc gcgcgattcc ggaaccttac ccaacggcaa cccgacggta
     1741 cgccccttgc tcctcccttc tccatccaaa ttcaccacgg gaggtgggga gatgagaagg
     1801 gcactcggga agaagcgcaa tgggcagggg ctcacggagt acatcatcat cgtggcgctc
     1861 gtggccgtcg cggggatcgg aatcgtcaac atcttcggca accagcttcg gaaccagttc
     1921 cacacgatcg taaaggcaat gtcgggcagc gagacggcca ccgtccaatc gctcgcgggg
     1981 aaagccggaa cggaagccaa ccagaaaaac ctttccacct atgccggctc gaagtgagcg
     2041 aggggcggcg atgatcgagt tccttctcgc gggagttccg ctccttctcc tgatgctggc
     2101 gattgttcag ttgtccctcc tctgggcggg gaagagcgcc gtcgacacgg cggctcacct
     2161 cgccgcgcgg aaattcgccc gcatcgccag ggaagacttc cgcaaggcac gcgagatggc
     2221 cttcctcgag gcgttccagg tgtgccggaa ccggcccggg ggatcgttcg ggtcggccgc
     2281 catgaccgcc cttgacgtca cgaaggacgg cgaacagggg gccaaccgcg ccgacgccgg
     2341 cgaagcgctc tgcatccgcc tgacccacgg cgtcgaactg gtcgtcccct ggatcgaccg
     2401 ggtcctgttc accctttccc cggggaagaa gctccgcctc ggggagcact attacctgat
     2461 gatgcaatcc acgcgctggg tgacggtcga gtgaccatga tcggatattt ctcgcgacag
     2521 gcggtacagc ttcgcgcggg aaaccgcggg caggcgacgg ccctcttcct ggtcgtcgcc
     2581 gccaccctcc ttgtggtggt cttcgcctcg atccgcctgc accatgtcgc ggtcgcgcgg
     2641 gtgagcacgg cggattcggt cgacgcgatc gccctgtcgg ccgccacctg ggaggcgcgg
     2701 tgcctgaacc tgatcgccgc gctgaacgac ggtgcgatcc aatgtttccg cgtcatccgg
     2761 tggacgtgcg tgctgtgggc ggcgctggcc gtcgccgcgg ccttcgggtt cggcgtcccg
     2821 gcgttcgcga tgtacaccag ggaagcccga aggctcatct ccgggtactg ggacacggcg
     2881 cacctgatgg tctcctggtc cgaaaagatc cggaaggcgg cgccgtacct ggtcctcggg
     2941 gaagttgcgg ccttgtcgaa acagcggaac gtcgccggga tcctgtttcc gttgaatccc
     3001 gccggtccgc acgatgggaa gaacacgctc gagctccacc tcgcccccgg tccgccgatc
     3061 ttccttctgg atgccatcgc gcccatcagc ggcgtcctga accaattgaa gaggatcccg
     3121 ttcctcaata gcgcgatgaa gacggtcatc gcgctgctcg acaccgcgct gcgcgggatc
     3181 gtcggaaccc gcaagggacc gatccggatg ctggaacccg agatcgattt tccggaccgg
     3241 caattcgtcc ggttcgaggg aatccacacg gttcccgacc tccccatccc cttcctcgac
     3301 gaacggggga agcgtcgggt aatcgaagat gcgacggccc aaccgtacgg aggaggaccc
     3361 gcggagatga cgtggagatc gaggctggcc gaacggggga agaaatgaag ggccgccgca
     3421 aatcgccccg tgggcaatct ctcgtcgaga cggtgctggt ggtccccctg ctcctgatcc
     3481 tgctcggcgg agggtactgg ttctaccgcg acctatcgct ctcttcttcc gcggagagcg
     3541 cagcacacgc acagatgctg cgggccggca ggcgcctttc ggggatcgag tcccgacttt
     3601 ccgggtcgat ccacccggga gacgacgtcg cgcgcatcga ggcgcacaac gatcccctga
     3661 tcggcgaggt ccccctgttc cgcgggctgg caggcagaac gatcgcgtcc gcaaacgtct
     3721 ccctcggaaa agaacccgtg ggcgcgttcc tcgacctccc gtcccacgct ctccgcaggg
     3781 aggcggaagg cgccgtggat tgctggggaa aggagacccc ctccggcgcg acggtccggc
     3841 ggacggttca gggaattctg ttgacggggg tgctccggtg aaccggaaga ttctcgccgg
     3901 actcgccggg gcggcaacgt tgttcgtgtc gctttgcaca gtggataccg gcaacgcccg
     3961 cagaaaagag atctcctcga cccgccgttt cgtcatcgac ggaagatcgt tcgactccga
     4021 cgaatcctcg ccggacaacg gttcgcttct gaagagggag ctcggcagga tgggcgtccg
     4081 cttgcccgat gggttccccc tgccggagga gtcggttctc tcgcacccgg cgttcgaagg
     4141 ccggctgaag gaatcgcggt caccggtcct tgaggcgccc cggctgcctg cgggattgac
     4201 cgcggagcac accctccgga tggaaggaga ggggatgccg gtcgacctcg ttttcggaaa
     4261 gcttggggcc ccgggacctt ccatccgttc ccgcctgctc ctctccggct ggaaatccgt
     4321 atccaccgcg aacggatccg gagaaacgca tgtgctgcaa atccgtcatg gaaaggaaac
     4381 cagcattgtt tgcctcgatg atgcggaagg cacgttcctt ctcttccggg aagtcggccg
     4441 gtaaggtccg gacgctgctc cccctgttcg cgggtcttct cctgtgcggt ttcgctctgg
     4501 cagccgccgg acgcagggtg gcgaccgtgg aaaaggacat cctccgacag gcgaatccgg
     4561 tggaggtggt cgtcgcctcg gtgccgattc ccgccggtga gacgttcggc gcgcggaacc
     4621 tcgcgaaaaa agcgatcccc tcgtccggga cggggcaacg gaacgtgccc gccaccgatt
     4681 tcgaacttct ggttggggcg cgcgccaaga cggcgatcga tcccggagag ccggtattgt
     4741 ggaccgacgt cgaggagccg tacgacacgg atgcgttctc caggatcgtc cttcccggcc
     4801 ggagggcgat gacgctcggg gtggacacga catcgtcgtt tgccggcctg gtccacccag
     4861 gggaccgtgt ggacctgctc gtggaacgta ccggggcgaa ttccgccgac tgggtccggg
     4921 acatcccggt gatcgccgtg gaccgggatc acaatcgcct cgcgcacccc accgacaaag
     4981 aggaggcgac caccgtaacg ctgatggtca gccccgcgga agggaaccgg atcgcacggg
     5041 cctccggaaa agtgcactgg ttccttcgca accccgacga caatgcggcc gtcgcctcac
     5101 tccgtccggc aaagccgatg acgccccatg tcgtggaagt gtggaaaggc ggggtgaaga
     5161 tcccgccgaa cttcgcggca aaggagaacc acgaatgaga cgccttcatc cgcgctccgg
     5221 gcgtgccttc gccgccttct ccctgatcct ggccttcgca tccggctccg catccatcgc
     5281 gtcggagacg atccggatcc gcccggggtt ccagcggatc ctcgaacgga cgggcgtgtc
     5341 gagactttcc gtc
//
LOCUS       DASVJL010000426         4090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1010108, whole genome shotgun sequence.
ACCESSION   DASVJL010000426 DASVJL010000000
VERSION     DASVJL010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010108"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VF357_08960"
     CDS             <1..518
                     /locus_tag="VF357_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524320.1"
                     /translation="IRRGLNDLLEREGTTLRLYHDRFRPYQDVLGFDEMAQAEETVRL
                     FDMVAAHRHMETSVAYLSRRPEPESRGAIREAVLAVTAVAREVAAREVREGEGEAPLI
                     VGSVAHAAAAAGIPSELLGGIEAILRRAHALSGLPVDGVESPAGEEPVDPREAHFLVV
                     FASSLIVYLKR"
     gene            609..1589
                     /gene="cysK"
                     /locus_tag="VF357_08965"
     CDS             609..1589
                     /gene="cysK"
                     /locus_tag="VF357_08965"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX7524321.1"
                     /translation="MATWFPDNSLSIGKTPLVKLNRITADAGATVLGKVEGRNPSYSV
                     KCRIGAAMVWDAEKKGLLGPGKTIVEPTSGNTGIALAFVAAARGYGIVLTMPETMSVE
                     RRQVLKAFGAKLVLTEGAKGMKGAIAKAEEIVASDPSRNFMPQQFKNPANPLVHETTT
                     GPEIWEATDGAIDVLVSGVGTGGTITGITRFIKNTKGKKILSVAVEPVHSPVITQQRN
                     GQPLMPGPHKIQGIGAGFIPDVLDLSLIDRVETVSNDEAIAFARRLAREEGLLSGISS
                     GAAAAVAVRLAKLPEFTGKTIVAILPDAGERYLTTPLFEGWFDTESAATI"
     gene            complement(1594..2301)
                     /gene="ubiE"
                     /locus_tag="VF357_08970"
     CDS             complement(1594..2301)
                     /gene="ubiE"
                     /locus_tag="VF357_08970"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX7524322.1"
                     /translation="MYRLTDRNRSIGEMFSSIAPRYDFLNRLLSLGVDRRWRRLAVAE
                     TVPSTGGRFLDVATGTADMALEILRQKGAGASVAGVDLSVEMMRVGQEKCARAGQSRR
                     VAFLRSPGECLPFGDGVFDSACVAFGIRNVADRERGLREMCRAVRPGGRVVVLEFSSP
                     PGSVFGALYRFYFRCVLPRIGGIFSRGSAYAYLPESVLAFPEPPAFAGMMRAAGCVSV
                     THRPLTFGIVTLYVGMR"
     gene            2438..>4090
                     /locus_tag="VF357_08975"
     CDS             2438..>4090
                     /locus_tag="VF357_08975"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX7524323.1"
                     /translation="MKRAALCIAASIAAFILAGVPPAHAQDEAGAEGGFSAYAVARVK
                     VLDGSVWVRPSGGGDWEEFSSNSPIPPRSRVSVPDGSEAELQFHGGQFVLLTSGTDLE
                     VRDLQEGKSVFRLRAGEIRFDLPADDFAPVSVRVPGGAVAQFSEPGRQWLTVTDSDET
                     RLVARRGGAVVTVGGDEHRLRAGQEAVIGRDVAVGEYRGDGEADESAEMPPPSEGEAL
                     GDAPPAVVDELQEYGEWVDVPTYGTVWRPRVAVGWSPYVYGHWTWISPYGWTWVSYEP
                     WGWYPYRCGYWLTDPVFGWIWSPFNAFVSVDFVFGSSRFRHHNVFFRPATVRFIREGG
                     NVRWVPLGPGERFRPAGFARGDSRLARWNRPLDSGRVFVRGGADRREWRDWSAVRAER
                     QVEIRKTHAAQPRPDTRTVRPERPGVRSTVRQPAQRTAPREDRRTGTPREAVPGPGKV
                     ERQAPSPRGVPAARPPESEKLRDVQQKDDRSGGTPAVREGGRGTVQERAPAYRSPDAG
                     SRGQEIRGNRGGDDGGRGYDRGGGDRGGGDWGGGDRGGGDRGG"
BASE COUNT          694 a         1248 c         1513 g          635 t
ORIGIN      
        1 tgatccgcag gggattgaac gacctgctcg agcgggaagg gacgaccttg cgcctctacc
       61 acgaccggtt tcgtccctat caggatgtcc tcggtttcga cgagatggcg caggccgagg
      121 aaacggtgcg gctgttcgac atggtcgcgg cgcaccgcca catggagacc tccgtggcgt
      181 acctttcccg gcgtcccgaa ccggagtccc ggggagcgat ccgggaggcg gttcttgcgg
      241 tgacggcggt ggcgagggaa gtggccgccc gggaggtccg ggagggggag ggagaagcgc
      301 cactaatcgt ggggtcggtg gcgcacgcgg cggcggcggc cgggatcccg tcggagctgc
      361 tgggggggat cgaagccatc cttcgccggg cgcacgcgtt gagcggcctg ccggtcgatg
      421 gtgtggaatc acctgctggc gaggaaccgg tggacccgcg cgaagcgcac ttcctggtcg
      481 tcttcgcctc ctcgctgatc gtctacctga agagatagcc cgacgggatg ttcggggtga
      541 gatttcctgt tgacagtcgg cccgtcgata aatactatat agactataga caataaggag
      601 gttataccat ggccacctgg ttcccggaca attcgctttc gatcgggaag actcccctcg
      661 tgaaactcaa ccggatcact gccgacgccg gcgcgacggt gctcggcaag gtggaggggc
      721 gaaatccctc ctactcggtc aagtgccgca tcggcgccgc gatggtttgg gacgcggaga
      781 agaaagggtt gctcgggccg gggaagacga tcgtcgaacc cacctccgga aacaccggga
      841 tcgccctcgc cttcgtcgcg gccgcccgcg gctacgggat cgtcctgacg atgcccgaga
      901 cgatgagcgt tgagcggcgg caggtgctca aggccttcgg tgccaaactg gtgctcaccg
      961 agggagcgaa ggggatgaag ggggcgatcg cgaaggcgga ggagatcgtg gcgtccgacc
     1021 cgtccaggaa cttcatgccg cagcaattca agaatcccgc caacccgctg gtgcacgaga
     1081 cgaccacggg accggagatc tgggaggcga ccgacggcgc gatcgacgtg ctcgtgtccg
     1141 gcgtcggcac cggcggtacg atcacgggga tcacccggtt catcaagaac acgaagggga
     1201 agaagatcct ctcggtggcg gtcgaaccgg tccatagccc cgtgatcacg cagcaacgga
     1261 acgggcagcc gctgatgccc gggccgcaca agatccaggg gatcggcgcg gggttcatcc
     1321 ccgacgtcct cgatttgtcg ctgatcgacc gcgtagagac cgtttccaat gacgaggcga
     1381 tcgccttcgc ccgacggctg gcgagggaag aggggctgct ctcggggatc tcgagcgggg
     1441 cggcggctgc ggtcgcggtg cgtctcgcga agcttcccga gttcaccggg aagacgatcg
     1501 tggcgatcct tcccgatgcc ggggagcggt atctcacgac ccccctgttc gaggggtggt
     1561 tcgatacgga aagcgccgcg acgatttaag atcttaccgc attcccacgt acaaggtgac
     1621 gatcccgaat gtcagcgggc ggtgggttac ggaaacgcag cccgccgccc gcatcatccc
     1681 ggcaaaggcg ggaggctcgg ggaacgcgag caccgactcg ggcaggtacg cgtaggcgga
     1741 accccgggag aagatccctc cgatccgcgg caggacgcac cggaagtaga accggtacag
     1801 ggcgccgaag accgaccccg gcggcgagga gaattcgaga acgaccaccc gcccgccggg
     1861 ccggacggcg cggcacatct cccgaaggcc ccgctcgcgg tccgccacgt tccggatccc
     1921 gaaggcgacg caggcggagt cgaagacccc gtccccgaaa gggaggcatt cccccggcga
     1981 acgaaggaac gcgactcttc gggattgccc cgcccgcgcg cacttctcct gccccacccg
     2041 catcatctcc acggagaggt cgacgccggc gacggacgct cccgcgcctt tctggcgcaa
     2101 tatctccagc gccatgtcgg ccgttccggt cgcgacgtcc aggaatcttc cgccggtcga
     2161 cgggacggtt tccgccacgg cgagacggcg ccagcgccgg tccaccccca gcgacagcag
     2221 ccggttgagg aaatcgtacc ggggagcgat ggaggagaac atctccccga tggaccggtt
     2281 gcggtcggtg agtcgataca attcgctgaa accccgtcgt ggtataaagg tttcaagtat
     2341 agcaggcttg gaatttcagc gaatcggggc gaaaagggtg aatattccct acgcgtcggt
     2401 ccgtctaacg ggcatgagga aagggaggga acgtgccatg aagcgcgccg ccttgtgcat
     2461 cgctgcatcg atcgccgctt tcattctcgc cggagtcccg cccgcccacg cccaggatga
     2521 ggcaggagcg gagggtgggt tctccgccta cgccgtcgcc cgggtcaagg tgctggacgg
     2581 ctcggtgtgg gtccgcccgt cgggcggcgg ggattgggag gaattctcga gcaacagtcc
     2641 gattcccccg cgcagccgcg tgagcgtgcc ggacgggtcg gaggcggagt tgcagtttca
     2701 cggcgggcag ttcgtcctgc tgacgtccgg gaccgacctg gaagttcgcg accttcagga
     2761 ggggaaatcc gttttccggc tacgcgcggg ggagattcgg ttcgaccttc ctgcggacga
     2821 cttcgccccg gtttccgtgc gggtccccgg tggggcggtt gcgcagttct ccgagccagg
     2881 gaggcaatgg ctgactgtga cggacagcga cgagacgcgg ctggtcgcgc gccgcggcgg
     2941 ggccgtggtc accgttggtg gtgacgagca tcgccttcgt gccggtcagg aggcggtaat
     3001 cgggagggac gtcgccgtcg gggagtatcg cggagacgga gaagccgatg aatccgcaga
     3061 gatgcccccg ccgtccgagg gggaggcgct gggcgatgct ccgccggcgg tggtggacga
     3121 actgcaggag tacggcgagt gggtcgacgt gccgacgtac ggaaccgtct ggcggccgcg
     3181 cgtggcggta ggctggtccc cctacgtgta tggtcactgg acgtggatct caccatacgg
     3241 ctggacctgg gtttcctacg agccgtgggg gtggtatccg taccggtgcg ggtactggct
     3301 gaccgacccg gtcttcgggt ggatctggtc tccgttcaac gcgttcgtat ccgttgactt
     3361 cgttttcggc tcaagccgat ttcggcacca caatgttttc ttccggcccg cgacggtacg
     3421 cttcatccgc gagggcggca atgtccgctg ggtgccgctg gggccggggg agcggttccg
     3481 cccggccgga tttgcccgcg gcgattcccg gctcgcgcga tggaaccgtc cgctcgacag
     3541 cggtcgggtc ttcgttcgcg ggggggcgga tcgccgcgaa tggcgcgact ggagcgccgt
     3601 gcgcgccgag cggcaggtgg agatccggaa gacccacgcc gcgcagccgc ggccggacac
     3661 ccgcacggtg cgtcctgaga ggccgggggt tcgctctacg gtcaggcaac cggcgcaacg
     3721 aaccgcgccg agggaagacc ggcggaccgg aacgcctagg gaggccgtgc cggggccggg
     3781 gaaggtggaa aggcaggcgc cttctccacg cggtgtgccc gcggctcgcc cgccggagag
     3841 tgagaaattg cgcgatgtcc aacagaagga tgatcgatcg ggcggaacgc cggctgtgcg
     3901 ggaagggggc cgggggaccg tgcaggagcg ggcgccggca tatcggagtc cggatgcggg
     3961 gtcgcgcggc caggaaatcc gcggcaaccg cggtggcgac gacggcggcc gagggtacga
     4021 tcggggtggc ggcgatcggg gtggcggcga ctggggtggc ggcgaccggg gtggcggcga
     4081 ccggggcggc
//
LOCUS       DASVJL010000427         4539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1199798, whole genome shotgun sequence.
ACCESSION   DASVJL010000427 DASVJL010000000
VERSION     DASVJL010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199798"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VF357_08980"
     CDS             <1..957
                     /locus_tag="VF357_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7524324.1"
                     /translation="SLDLITSAAASILRVERCVLLLPEPGVGHLVVRSMAGIPRARQF
                     EKYRQEMYKLVVHPILSSGTGMIVSEGRPGTDRVLLRLMRRLEVKGFLAAPVKSPTAA
                     IGIMAAATPLDGRDLQDGDLKLLSVLANFAAIALENAALIGHLDKKARKLTAIFEISK
                     ALNEQSDPAVLFQLIVDRATDLMDASSGSVIRRDPDSETLFIEAERGLGPEVKNAMRL
                     RVGEGITGWVAREGEPLLVPDVRKDPRYVQANPAVMSEMAVPVKWGSEVMGVINLDHH
                     HAGGFTEEDLELLTAFGNAAAVALRNANALCGWPRKAGPGKE"
     gene            954..1898
                     /locus_tag="VF357_08985"
     CDS             954..1898
                     /locus_tag="VF357_08985"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7524325.1"
                     /translation="MTPVHGTILVARPLWPVLGRVGILLSRRGYTVEKAGSWSALLAE
                     PGRRAGLAAVLLGEYGRVAEEEDILRRFRDDAGPGIPVLLIGGLSAVRWSRRLREAGA
                     DMVFAADLPEEEILDRAQPLLAYGEMYRKAVLAGRELSDQSMRDRLTGLPDRRGFSLD
                     LDRCGETARRIGRPLSCIVTDIDDLRRVNETYGPETGDSVIRQFGGVLAQVKRSYDTV
                     ARLGGDEFVWLLLGVGRGEALRAAERAQRLVSGSVFNGSHAPVSVTATFGVASLSPGG
                     EWNTQALVENADRALYWGKESGKNMVRFYPPGKEVKGA"
     gene            1891..2364
                     /gene="def"
                     /locus_tag="VF357_08990"
     CDS             1891..2364
                     /gene="def"
                     /locus_tag="VF357_08990"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX7524326.1"
                     /translation="MRREILIYPHPFLARKAVPVATVDDRIRALIRDMFVTMYAAEGI
                     GLAASQVGVGKRVVVLDVSPVDETIAPVAVVNPEIVSRDGSATSVEGCLSVPGVQGEV
                     CRAETVEVRGMDERGNPLRIRAAGIFSRALQHEIDHLDGILFIDRLSSSAAASAK"
     gene            2361..3326
                     /gene="fmt"
                     /locus_tag="VF357_08995"
     CDS             2361..3326
                     /gene="fmt"
                     /locus_tag="VF357_08995"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX7524327.1"
                     /translation="MIRPSPGPGAARMRTVFMGTPRFAVSSLAALAEAVDVTLALCNP
                     DRPAGRGRSMASPPVKEEAVRRGIPVFQPEKVRHPDAVARIVAEAPDLIVVVAYGHIL
                     PKSILDIPRLGCINVHASLLPKYRGAAPINWAVARGETVTGITIMRMDEGMDTGPMLY
                     VREMPIGEEDTAETMFSKLSILGAKALHEALRRLGEGTLAETPQDAALATYAPMLKKE
                     HGRIDWTRPAGEVCNLVRGMAPWPSAFALHAGKTLKVLSSAVSGESGAAGEPGEIVVL
                     GHDGISVACGEGVLRLQVVQPEGRKAMDAWAYAQGRRVATGDRLS"
     gene            3275..>4539
                     /gene="rsmB"
                     /locus_tag="VF357_09000"
     CDS             3275..>4539
                     /gene="rsmB"
                     /locus_tag="VF357_09000"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:TIGR00563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX7524328.1"
                     /translation="MGVRPGTARGDGGPAIVRDAAISVLARVDRDEAFADIVLDRALR
                     DAGFSDSRDRGLLTELVMGTLRRRGTIDFALLPFLSRPLEQIDAYVRNALRVGAYQLF
                     YTRIPDRAALNETVAAVKAARGGGAGGFVNAVLRGIVRAGKIPVLPPEGDPRRLPAEG
                     SAPRDLIDAFTTSLGEAETRAYLAACLEKPPFAVRANPFRVVTEALARRFADEGKDPA
                     PCRFAPDGFVLGKPAPVFSDAAFLEGAYLVMDEGAQLIATLLRPEPCERILDACAAPG
                     GKTTHLSALAGGKAEILATDVSALRLRMLREVMTRTGAPGIRTALHDLSRAPMTPSSG
                     LFDKALVDAPCTGMGVIRRNPDAKWRFRPDGPRRMARVQAAILRNAWEAVRQGGLLLY
                     CTCSPLKEEDEEVVAAFLAERPEAAVAARP"
BASE COUNT          672 a         1483 c         1673 g          711 t
ORIGIN      
        1 agcctcgacc tcatcacctc cgccgccgcg tcgatcctcc gggtcgagcg gtgcgttctc
       61 ctcctcccgg agcccggggt ggggcacctg gtggtccggt cgatggcggg gatcccgcgg
      121 gcgcggcagt tcgagaagta ccggcaggag atgtacaagt tggtcgtcca tccgatcctc
      181 tcctccggga cggggatgat cgtctcggag gggaggccgg ggacggaccg cgtcctcctg
      241 cgactgatgc gccgtctgga ggtgaagggg ttcctcgccg caccggtgaa gagtcccacg
      301 gcagcgatcg ggatcatggc cgccgcgacg ccgctggacg ggcgggatct gcaggacggg
      361 gacctgaaac tcctctccgt ccttgcgaac ttcgcggcga tcgccctcga gaacgcggcg
      421 ctgatcgggc acctcgacaa gaaagcgcgc aagctgaccg cgatcttcga gatcagcaag
      481 gcgttgaacg aacagagcga cccggccgtc ctgttccagc tgatcgtcga ccgcgcgacg
      541 gatttgatgg atgcctcttc cgggtcggtc atccggaggg accccgattc ggagacgctc
      601 ttcatcgagg cggaacgagg cttgggcccc gaggtgaaga acgcgatgcg gcttcgcgtc
      661 ggggagggga tcaccgggtg ggtcgcgcgg gaaggggagc cgttgctcgt tcctgacgtg
      721 cgaaaggatc cccgctacgt ccaggcgaat cccgcggtga tgtccgagat ggcggtgccg
      781 gtaaaatggg gatccgaggt gatgggggtc atcaacctcg accatcatca tgcgggagga
      841 ttcaccgagg aggacctcga actgctcacg gcgttcggca acgcggcggc ggtggcgctg
      901 cgcaacgcca atgctctctg cggatggccg cggaaggcgg ggcccgggaa agaatgacac
      961 ccgtgcatgg aaccattctc gttgcccgcc cgttatggcc cgtcctcggg cgcgtcggga
     1021 tcctgttgtc ccgccgcggt tacacggtgg agaaggccgg ttcctggagc gcgctcctcg
     1081 cggaaccggg aaggcgggcg gggctcgcgg cggtcctgct gggggagtac ggaagagtcg
     1141 cagaggaaga ggatatcctg cggcggttcc gtgacgacgc cggacccgga atccccgtgc
     1201 tcctgatcgg agggctgagc gcggtccggt ggtctcgtcg cctccgggag gcgggggcgg
     1261 acatggtctt cgccgcggat cttccggaag aggagatcct cgaccgtgcg cagccgctcc
     1321 tcgcgtacgg ggagatgtac cggaaagccg tcctcgccgg ccgggagctt tcggaccagt
     1381 caatgcgcga caggctgacc gggttgcccg accggcgcgg tttctcgctc gatctcgacc
     1441 ggtgcggcga gacggcgcgc cgcatcgggc ggcctctctc ctgcatcgta acggatatcg
     1501 acgacctccg gcgcgtcaat gagacgtacg ggccggagac gggggacagc gtgatccggc
     1561 aattcggcgg cgtactggcc caggtgaaga ggagttacga caccgtggcc cgcctgggag
     1621 gcgacgagtt cgtctggctc cttctgggcg tcgggcgcgg agaggcgttg cgggccgcgg
     1681 agagggccca gcggctcgtt tccgggagcg tcttcaacgg gagccacgca ccggtcagcg
     1741 tgaccgcgac cttcggcgtg gcgtctctct cccccggggg ggagtggaac acgcaagccc
     1801 tcgtggaaaa cgccgaccgc gccctgtact gggggaagga gagcggaaag aacatggtac
     1861 ggttctaccc tccagggaag gaggtcaagg gtgcgtaggg agatcctgat ctacccccat
     1921 ccgttcctcg cgcggaaggc ggtccccgtg gccacggtgg acgaccggat ccgcgccctc
     1981 atccgcgaca tgttcgtgac gatgtacgcc gccgagggga tcggactcgc cgcgtcccag
     2041 gttggggtgg ggaagcgggt cgtcgtcctc gatgtttccc ccgtggacga gacgatcgcc
     2101 cccgtggccg tggtgaaccc cgagatcgtc tcgcgggacg gatcggccac gagcgtcgag
     2161 gggtgcctga gcgtccccgg ggtccagggc gaggtgtgcc gcgccgaaac cgtcgaggtg
     2221 cgcggcatgg atgagcgggg gaatccgctt cggatccgtg cggccgggat cttctcccgc
     2281 gcgctgcagc acgagatcga ccatctcgac gggatcctct ttatcgatcg tctttcctcc
     2341 tccgcggcgg cctccgcgaa gtgatccgcc cgtcgcccgg tcccggcgcc gcccggatgc
     2401 gcacggtgtt catgggaacg ccgcggttcg ccgtctcttc cctcgccgcc ctcgcggaag
     2461 cagtggacgt gactctcgcc ctgtgcaacc cggaccggcc cgcggggcgc ggacgatcga
     2521 tggcgtcgcc cccggtcaag gaggaggcgg tgcggcgcgg gatcccggtc ttccagccgg
     2581 aaaaggttcg acaccccgat gccgtcgccc ggatcgttgc cgaggcgccg gacctgatcg
     2641 tcgtcgtggc gtatggccac atcctgccga aatcgatcct cgatatcccc cgtctcgggt
     2701 gcatcaacgt ccacgcctcc ctgctcccga agtaccgcgg cgcggccccg atcaactggg
     2761 cggtggcgcg gggcgaaacg gtgactggga tcacgatcat gcggatggac gaggggatgg
     2821 acacggggcc catgctgtat gtgcgggaga tgccgatcgg cgaggaggac accgcggaga
     2881 cgatgttttc caaactgtcg attctcgggg ccaaggcgct gcacgaagcg ttgcgcaggc
     2941 tgggggaggg gacgctcgcc gagacgccgc aggacgccgc ccttgcgacg tacgccccga
     3001 tgctgaagaa ggagcacggc cggatcgatt ggacccgtcc ggcgggggag gtgtgcaacc
     3061 tggtccgcgg gatggcgccg tggccctccg cgttcgcgct tcacgccggg aagacgctca
     3121 aggtcctctc gtcggccgtc tccggggaat cgggcgcggc gggggagccc ggcgagatcg
     3181 tcgtcctcgg gcacgacggg atctcggtcg cctgcggaga aggggtcctc cgtcttcagg
     3241 tcgtgcagcc ggaggggagg aaggcgatgg acgcgtgggc gtacgcccag ggacggcgcg
     3301 tggcgacggg ggaccggcta tcgtaaggga cgcggcgatc tcggtcctgg cccgggtgga
     3361 ccgcgacgag gccttcgccg acatcgtgct cgaccgcgcg ctgcgcgacg cgggcttctc
     3421 cgattcccgc gaccgggggc tcctgacgga gctcgtgatg ggaaccttgc gccggcgcgg
     3481 cacgatcgat ttcgcccttc tgccgttcct ctcccgtccc ctcgaacaga tcgacgcgta
     3541 cgtccggaac gccctccggg tcggggcgta ccagctcttc tatacccgca tccccgaccg
     3601 cgcggcgctg aacgaaacgg tggcggccgt caaggcggcc cgaggcggcg gcgcgggggg
     3661 gttcgtcaac gcggttcttc gcgggatcgt ccgcgcgggg aagattcccg tccttccccc
     3721 ggagggagac ccccgccggc ttcccgcgga gggatcggcc ccgcgggatc ttatcgacgc
     3781 gttcacgacg tcgctggggg aggcggagac gcgggcgtac ctcgccgctt gcctcgagaa
     3841 accgcccttc gcggtgcgcg ccaacccgtt ccgggttgtc acggaggcgt tggcgaggcg
     3901 attcgccgac gaagggaaag accccgcccc ctgccggttc gcgccggacg gattcgtcct
     3961 cggaaagccc gccccggtct tctccgacgc tgcgttcctc gaaggggcgt acctcgtcat
     4021 ggacgaaggg gcgcagctga tcgccaccct gcttcgtccc gagccgtgcg agcggattct
     4081 cgacgcctgc gccgcccctg gcggaaagac gacgcacctg tccgccctcg ccggggggaa
     4141 ggcggagatc ctcgcaacgg acgtctccgc cctgaggctc cgcatgctgc gcgaggtgat
     4201 gacgcggacc ggcgcgccgg ggatccggac ggccctccac gacctctccc gggctccgat
     4261 gacaccttcc tccgggctct tcgacaaggc attggtggac gccccgtgca cggggatggg
     4321 ggtgatccgc cggaacccgg acgccaagtg gcggttccgg ccggacgggc cgcggcggat
     4381 ggcgcgggtc caggcggcga tcctgcggaa cgcgtgggaa gccgtgcgcc agggcgggct
     4441 cctgctctac tgcacctgtt cgccgttgaa ggaggaggac gaggaggtcg tcgcggcgtt
     4501 cctcgcggag cgcccggagg cggccgtggc ggcccgtcc
//
LOCUS       DASVJL010000428         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_166730, whole genome shotgun sequence.
ACCESSION   DASVJL010000428 DASVJL010000000
VERSION     DASVJL010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166730"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VF357_09005"
     CDS             complement(<1..440)
                     /locus_tag="VF357_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524329.1"
                     /translation="MSALVTLPVWVVTVLALLAALTAVDRLLLPGVRWFLRRRVNIVL
                     DEIGKRLHLEIRPFTLTKRQVLIDRLMYDPKVLEAAGAHAIERGIPRDVVMAEVERYA
                     REIVPAFNAYAYFRLGYWLARRAARLLYRVRVGFTDEKGLSGVDP"
     gene            493..1125
                     /locus_tag="VF357_09010"
     CDS             493..1125
                     /locus_tag="VF357_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7524330.1"
                     /translation="MGGNPVESKVIEAVVLVHGLWMTGTEMRVLGGRLEESGFRVRYF
                     RYRSWRGGLAEAAGALREFVEATEGERVHLVGHSLGGVVIAKMLEEAPLSRPGRVAML
                     GSPMGGSAAARIVSRRRVGRWLVGRVIREGIVEHAPKWPEGRELLVVAGDIPLGSGLL
                     LGLHKPHDGVIRVEETRVEGARMVIARASHVGLLLSRKVASLLSDHFRSA"
     gene            1284..1751
                     /locus_tag="VF357_09015"
     CDS             1284..1751
                     /locus_tag="VF357_09015"
                     /inference="COORDINATES: protein motif:HMM:NF028509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX7524331.1"
                     /translation="MRRITLAVLGIAVSLCLAGSAFAAGATKDECVSKSKEAAAMINA
                     KGLDAAIAEINKKDGKFVWKDTYVFLMDLDGKMLAHPMSPALIGKNVLDMKDKGEPGK
                     LLFKEFVVVAKGKGEGWVDYMWINPGDPKPRKKVTYIYRVPGKDLFVGAGIYE"
BASE COUNT          367 a          546 c          703 g          341 t
ORIGIN      
        1 gggtccacgc cggagagccc cttttcgtcg gtgaacccga ccctcacccg gtacagcagt
       61 ctcgcggcac gacgggcgag ccaatacccc agtcggaagt aggcgtaggc gttgaaggcg
      121 gggacgatct cccgggcgta ccgttctacc tccgccatca cgacgtccct cgggattccg
      181 cgctcgatcg cgtgcgcccc ggccgcctcg agcactttcg ggtcgtacat caggcggtcg
      241 atgagaacct ggcgcttggt cagggtaaag gggcggatct cgaggtgcag ccgtttgccg
      301 atctcgtcga gtacgatgtt cacgcggcgt cgaaggaacc agcggacacc gggaagaagc
      361 agtcgatcca cggccgtcag ggccgccagc agggcgagaa cggtcacaac ccataccgga
      421 agggtcacaa gtgcgctcac cggcacagtc taccatcaac cgatgggata taatcccggc
      481 cgggagggcg tcgtgggcgg gaacccggtg gaatcgaagg tgattgaagc ggtcgtcctc
      541 gtacacgggc tttggatgac ggggacggag atgcgcgtcc tcggaggacg cctggaggag
      601 tcgggtttcc gcgttcgcta cttccggtac cgctcctggc ggggggggct cgcggaggct
      661 gctggcgcct tgcgggagtt cgtcgaggcg acggaagggg agcgggtgca tctcgtcggg
      721 cacagcctgg ggggggtcgt gatcgcgaag atgctcgagg aagcccccct ttcgcggccg
      781 ggtcgcgttg cgatgctggg gtctccgatg ggcggaagcg cggcggcgag gatcgtgtcc
      841 cggcgccggg tcgggcgatg gctcgtgggc cgcgtgatcc gggaggggat cgtcgaacat
      901 gcgccgaagt ggccggaggg aagggaactt ctcgtcgtcg ccggggacat tccgttgggg
      961 tcggggcttc tcctcggctt acacaagccc cacgacggcg tcatccgtgt cgaagaaacg
     1021 cgcgtcgaag gagcgagaat ggtcatcgct cgggcatccc acgtcgggct gctcctttcc
     1081 cggaaagtgg cctcactgct ttccgaccac ttccggtccg cctgaccgaa tcccatccgg
     1141 ggtgacccga ccgacgaata tttcgcctgg accgatgggc aaacggggtt tcaatctggt
     1201 agattgattc gggaaaggag acctggggga aatggcaggt tgtgaataac ggcgttttac
     1261 gaaccgcaag aggaggttgt gggatgagga gaatcacgct ggccgtcttg ggaatcgcag
     1321 tgagcttgtg cctcgccgga agcgcgttcg cggcgggcgc cacgaaggac gagtgcgtgt
     1381 ccaagagcaa ggaagccgcc gcaatgatca atgcgaaggg cctggacgcg gccatcgcgg
     1441 agatcaacaa gaaggacgga aagttcgtat ggaaggacac ctacgtcttc ctgatggacc
     1501 tcgatgggaa gatgcttgcg catccgatga gtccggcgct gatcgggaag aacgtgctgg
     1561 acatgaagga caagggggag cccgggaagc tcctcttcaa ggagttcgtc gtggtcgcga
     1621 aggggaaggg cgagggctgg gtcgactaca tgtggatcaa tccgggcgat ccgaagccgc
     1681 gcaagaaggt cacctatatc tacagggtgc cggggaaaga cctgttcgtc ggcgcgggga
     1741 tctacgagta gtccgcgatc ccgcgggacc tttcgatccg ggccgggagg gatcctcccg
     1801 gctctttttt taccggggat catctcgtcg gtttcgattg catccggcgg gaaaggacga
     1861 agaagtggtg tcccatgatg gccagcgtta ccgccagccc aaggtgtctc ggcgcgcgga
     1921 tgatggtttt cccgaggaaa cgccagtacg ccacccg
//
LOCUS       DASVJL010000429         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_447566, whole genome shotgun sequence.
ACCESSION   DASVJL010000429 DASVJL010000000
VERSION     DASVJL010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447566"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VF357_09020"
     CDS             <1..620
                     /locus_tag="VF357_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7524332.1"
                     /translation="KTHKGITAFVVDMKWEGISLGKHEKKMGIKGSATSSIIFEEVRV
                     PAKNRLGNEGDGFKVAMNTLDGGRIGIASQAIGIARASFEDALSYAKDRKQFGQAICE
                     FQAIQWMLADIATEIDAARLLTWRAAWMKDRKMRHSKESSMAKLYASEVAMRAAVKGV
                     QIHGGYGYINEYPAERHFRDAKITEIYEGTSEIQRLVISAALLKG"
     gene            627..1757
                     /locus_tag="VF357_09025"
     CDS             627..1757
                     /locus_tag="VF357_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7524333.1"
                     /translation="MDFRLTDEQLQVEEMVRALSRKEFAPRAAQVDEESRYPAENHRR
                     LAELGLLGMLYPASFGGAAAGPVSYAIALREVAAGCASTGVGMAVTNMTGETIFRFGS
                     DAQRSLYLPMLSGGKGAGAFAITEPGAGSDAGGLSTFAREDGDGYVLDGSKVFITNGA
                     NACVSIVLALTRKSPRQISAFLVEPGTPGFSVGKGERKMGIKGSDTVSLFFEECRVPK
                     SSMLGAPGEGMKIALSALDGGRIGIASQAIGIARAALSTATEYARDRRQFGQAIGEFQ
                     AIQWKLADAATELDAAQLLAFRAASLKEKGVTYSKEASMAKLFATEAGNRACHAAVQV
                     LGGYGYIREYPVERHLRDIRVTTIYEGTSEIQRLVISRALCG"
     gene            1765..2304
                     /gene="cobO"
                     /locus_tag="VF357_09030"
     CDS             1765..2304
                     /gene="cobO"
                     /locus_tag="VF357_09030"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX7524334.1"
                     /translation="MSGGAVGKGYVQVYTGNGKGKTTASLGLAVRAAGHGLKTVIIQF
                     MKGWIDYGELAGVRMLAPYVEIHQAGRDTFVNRKNPDPEDLRLAREGWKLATETISGR
                     KADIVVLDEVNCAMDFGLLPVGDVLEAIKGKPDGMELVLTGRGAPPEIIEAADLVTEM
                     REIRHYYSKGVDARVGVER"
     gene            2319..>3243
                     /locus_tag="VF357_09035"
     CDS             2319..>3243
                     /locus_tag="VF357_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX7524335.1"
                     /translation="MYDRKTLAGIATRRKKWHDEALEPSLRKSPQRLERFSTVSDEEI
                     DLLYTPDPLSNFEYEEDLGFPGQYPYTRGVQPTMYRGRLWTMRQFAGFGSAEDTNARF
                     KFLLSQGQTGLSTAFHFPTLMGYDSDSPRARGEVGMCGVAVDSLKDMEILFDGIPLDK
                     VTTSMTINGPAAMVFAMYLAVAEKQGVPFHKVGGTIQNDILKEYIAQHSWIFPPEPSM
                     RITTDILAYCADNVPRWNTISISGYHIREAGSTAVQELAFTIADGIAYVQAGIDAGIP
                     VDKFAPRLSYFFNAHMDFFEEIAKYRAARRMW"
BASE COUNT          623 a          986 c         1117 g          517 t
ORIGIN      
        1 caaaaacgca caaggggatc accgcgttcg tcgtcgacat gaagtgggag gggatctcgc
       61 tcgggaagca cgagaagaag atggggatca aggggtccgc cacctcttcg atcatcttcg
      121 aggaggtccg cgtcccggcg aaaaaccggc tgggaaacga gggggacggg ttcaaggtcg
      181 ccatgaacac cctcgacgga gggcggatcg ggatcgcctc ccaggcgatc gggatcgccc
      241 gcgcctcgtt tgaggacgcc ctctcctacg cgaaggaccg gaagcagttc ggccaggcga
      301 tctgcgaatt ccaggcgatc cagtggatgc tggcggacat cgcaaccgag atcgacgccg
      361 cgcgtctgct cacctggagg gccgcctgga tgaaggaccg caagatgcgg cactccaagg
      421 agtcgtcgat ggcgaagctg tacgcctcgg aggtggcgat gcgcgcggcg gtgaaaggcg
      481 tgcagatcca cggcgggtac gggtacatca acgagtaccc ggcggagcgc cacttccgcg
      541 acgcgaagat cacggagatc tacgagggaa cctccgagat ccagcgcctg gtaatctctg
      601 ccgcgcttct gaagggatag ggcgcgatgg attttcgcct caccgacgaa caactgcagg
      661 tcgaggagat ggtgcgcgcc ctgtcccgga aggagttcgc cccgcgcgcg gcgcaggtcg
      721 acgaggagag ccgctatccg gcggagaacc accggcgcct ggcggagctc ggcctgctcg
      781 ggatgctcta cccggcatcc ttcggtgggg cggcggcggg gcccgtctcc tacgccatcg
      841 cactgaggga agtcgcggcg ggatgcgcct cgacgggagt ggggatggcg gtcaccaaca
      901 tgaccggcga gacgatcttc cggttcggca gcgacgcgca aaggagtctc tatctgccga
      961 tgctctcggg cggaaagggc gccggcgcgt tcgccatcac cgaacccggt gccggttccg
     1021 acgccggggg cctttcgacg ttcgcacggg aagacggcga cgggtacgtc ctcgacggct
     1081 ccaaggtctt catcacgaac ggcgccaacg cctgcgtctc gatcgtcctc gcgctgacgc
     1141 ggaagtcgcc gcggcagatc agcgctttcc tggtggagcc ggggactccg gggttctccg
     1201 tcgggaaagg cgagcgcaag atggggatca aggggtccga caccgtgtcc cttttcttcg
     1261 aggagtgccg ggtgccgaaa tcgtcgatgc tgggcgcccc cggggaaggg atgaagatcg
     1321 cgctttcggc gctcgacggc ggacggatcg gcatcgcgtc gcaggcgatc gggatcgcgc
     1381 gggcagcgct ttccacggcg acggagtacg cgagggaccg gcgtcagttc ggacaggcga
     1441 tcggcgaatt ccaggcgatc cagtggaagc tggcggacgc cgcgaccgag ctcgacgccg
     1501 cccagttgct cgcgttccgg gccgcgtccc tgaaagagaa gggcgtcacg tactcgaaag
     1561 aggcctcgat ggcaaagctc ttcgccacgg aagcgggaaa ccgcgcgtgt cacgccgcgg
     1621 ttcaggtgct gggcgggtac ggctacatcc gggaatatcc ggtggagcgt cacctgcgcg
     1681 atatccgggt gaccacgatc tacgagggga cctccgagat ccagcggctg gtgatctcgc
     1741 gggctctctg cgggtaggcg caacgtgagc gggggagcgg tcgggaaagg gtacgttcag
     1801 gtctacaccg ggaacggaaa agggaagacc acggcctccc tcgggctcgc cgttcgcgcc
     1861 gccggccacg gcctgaagac ggtcatcatc cagttcatga aggggtggat cgactacgga
     1921 gaactcgcgg gtgtccggat gctcgctccc tatgtcgaga tccaccaggc ggggcgcgat
     1981 accttcgtaa atcgaaaaaa cccggacccc gaggaccttc ggcttgcccg cgaaggctgg
     2041 aaactggcga cggagaccat ttcgggccga aaggcggata tcgttgttct cgacgaggtc
     2101 aattgcgcga tggatttcgg cctcctcccc gtcggtgacg tcctcgaggc gatcaaaggg
     2161 aagcccgacg ggatggaact cgtcctcacg gggcgcgggg ccccgccgga aatcatcgag
     2221 gcggcggatc tcgttaccga gatgcgcgag atacggcact attactcgaa aggcgtggac
     2281 gcccgcgtgg gcgtagaacg gtagggggag gcggcgccat gtacgacagg aaaacgctcg
     2341 cggggatagc gacccgcagg aagaaatggc acgacgaggc gctggagccg agccttcgga
     2401 aatcgccgca acgtctcgaa cggttttcca ccgtttccga cgaggagatc gacctcctgt
     2461 acacccccga tcccctttcg aacttcgaat acgaggagga cctcgggttt cccggccagt
     2521 acccgtacac ccggggagtg cagcctacga tgtaccgggg ccggttgtgg acgatgcgcc
     2581 agttcgccgg attcgggtcg gcggaggata cgaacgcgcg tttcaaattc ctcctctcgc
     2641 agggtcagac cggactttcc accgcgttcc actttccgac gctgatggga tacgactccg
     2701 actctccccg cgcgagggga gaggtcggga tgtgcggagt cgccgtcgat tcgctgaagg
     2761 acatggagat ccttttcgac gggataccgc tggacaaggt caccacctcg atgacgatca
     2821 acggcccggc ggcgatggtc ttcgccatgt acctcgcggt cgcggaaaaa cagggagtgc
     2881 ccttccacaa ggtcggcggc acgatccaga acgacatcct caaggagtac atcgcccagc
     2941 attcgtggat cttccccccc gagccgtcga tgcggatcac caccgacatc ctcgcctact
     3001 gcgcggataa cgtcccgaga tggaacacga tcagcatcag cgggtaccac atcagggaag
     3061 cggggtccac ggcggtccag gagctggcct tcacgatcgc cgacgggatc gcctatgtcc
     3121 aggcggggat cgacgcgggg attcccgtgg acaaattcgc cccacggctg tcgtacttct
     3181 tcaacgccca catggacttt ttcgaggaga tcgcgaagta ccgggccgcg cgccggatgt
     3241 ggg
//
LOCUS       DASVJL010000430         5249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_260143, whole genome shotgun sequence.
ACCESSION   DASVJL010000430 DASVJL010000000
VERSION     DASVJL010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260143"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="VF357_09040"
     CDS             <1..1567
                     /locus_tag="VF357_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX7524336.1"
                     /translation="SVRIMDAPVRAGRADADILLALEPTHLSHFLSGLHGQGFALTDL
                     PAGSAPDKVLVAPLKELAASAGSPILGNMVGAGIVAFALGVPLDIPDRLLAIEFRGDF
                     LEKNRKAIRLGAEWASGRVTETFRLPAAPFRPRLLLSGNEALALGAVAGGCKFAAGYP
                     MTPGSGILLGLSSDGPPLGLVFEQAEDEIAALNMAIGASYAGARAMVATSGGGFALMV
                     EALSLAGMLETPVVIALGMRPGPATGMPTRTGQEELEFALSAGHGEFPRLVLAPGSVE
                     EAYALAYHAMEMAEAHQAPCIILTDQYLADTVVDVDPEALAVLPVHRRIVRGNDVPRN
                     PEGRYLRYAITASGVSPMAVPGEPGITVVADSDEHGEDGHLTEDHGIRVRMVEKRARK
                     EQGLEATGLPPLLAGPPDGEAALVGFGSTKGVIAEAREILAREGVSVAAVHLRQVWPF
                     PAGAVEEILSRYGTTLTVENNRRGQLARLIRRETGRTVSGTVSRFDGLPFAPDEVARE
                     VKERIWAERSTRR"
     gene            1540..2400
                     /locus_tag="VF357_09045"
     CDS             1540..2400
                     /locus_tag="VF357_09045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7524337.1"
                     /translation="MGGTFDTAIESAWCPGCGNFSILTAVKKALERTGKEKHEIVLVS
                     GIGQAAKLPHYVDVNVFNGLHGRALPAALAIKMANPSLEVVVTSGDGDIYGEGGNHFV
                     HNIRRNPDIAVFVHDNQVYGLTKGQPSPTSDPGWTGSLQRSGVIAGPFPPLAVAIALG
                     CGFVARALASDVDHTAELMYQAIAHKGFALVDILQPCVTFNKKNTYQWYGENTYRVPP
                     EHDPADRERAFALSLSWGERIPVGILYRRERPCFEEVHPGIGDPPLHARETPEEAVRS
                     MIGKRVLILP"
     gene            complement(2408..4072)
                     /locus_tag="VF357_09050"
     CDS             complement(2408..4072)
                     /locus_tag="VF357_09050"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEX7524338.1"
                     /translation="MTYGFHLHLHVNGKRGVDLLHDPHINKGTAFTEKERDALGLRGL
                     LPPRVSTLEQQVSRVLENIHRKPSDIEKFIYLVSLQDRQETLFYRVVMDNIEEMMPII
                     YTPTVGQGCTEFGHIFRRPRGLYLSFKNQGRFREILTNWPYHDIRIIVVTDGERILGL
                     GDLGANGMGIPVGKLSLYTACAGIHPTQCLPVMLDCGTNNKTLLNDPLYIGHPSPRLR
                     GEAYDELVEEFFLAVRAIFPRACIQLEDFGNANAFRLLNKYKDRACMFDDDIQGTGSV
                     ALAGLYSALRITGNRLTDHKFLFLGAGEAGIGIGDLIVTGLMAEGMPETEAKRRCWYV
                     DINGLIVKSRTDLAPHHLPFAHDHAFIGDFRQAVEAINPTAIIGVSGAPGMFTRPIVE
                     AMARMNQRPIIFALSNPTSKSECTAEDAYTWSEGRAVYASGSPFEPVTYKGKTFVPGQ
                     SNNSYIFPGVGLGVIASEATRVTEEMFFAAAKTLSGMVTEDEYAMGRIYPRLDRIREV
                     SVSIAVAVAEVAFRRGLTKMHRPDDLMKHVKAQLYDPTYVEYTPKS"
     gene            complement(4369..5097)
                     /locus_tag="VF357_09055"
     CDS             complement(4369..5097)
                     /locus_tag="VF357_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7524339.1"
                     /translation="MTTFLQWIFVGVVTVILLPPFIFLGFFRKGLTGKGSLFRPLAKF
                     YSKVLYTAFRVRVVRLGFERIDPQRSYVFMPNHTSIADPLAMAIAIPQPFHFVFKKEL
                     ARIPVFGWALLSLGQIMVDRENTDQARASIAGAISGLAGNNSFLIFPEGGVSQDGQLL
                     PLKKGGFHLAIQTGLPIVPVRIEGARKVCPPGGSGHFHKGVISVRVFPPLTVCGKTET
                     DIPALVENVRACLTLDGPGPSPVP"
BASE COUNT          951 a         1647 c         1697 g          954 t
ORIGIN      
        1 ctccgtgcgc atcatggacg ccccggtccg cgcgggccgg gcggatgcgg atatcctcct
       61 tgccctcgag cccacgcacc tttcccactt cctgtccggc ctccatgggc aagggtttgc
      121 cctgacggat ctcccggccg ggagcgcgcc ggataaggtc ctggtcgcac cgctgaagga
      181 acttgcggcc tccgcgggga gcccgatcct cggcaacatg gtgggggcgg ggatcgtcgc
      241 ctttgccctg ggcgttcctc tcgacatccc ggatcggctc ctcgccatcg aattccgcgg
      301 tgattttctc gaaaagaacc ggaaggcgat tcggttgggg gccgaatggg cttcggggcg
      361 cgtcaccgag acgttccggc ttcccgcggc gccgttccgc ccccgcctgc tcctctcggg
      421 aaacgaagcc ctcgctctcg gcgcggtggc gggaggatgc aagttcgccg cggggtaccc
      481 gatgaccccc gggagcggca ttcttctcgg gctctcgtcc gacggtcccc cgctcgggct
      541 ggtcttcgaa caggcggagg acgagatcgc ggccctcaac atggcgatcg gcgcttctta
      601 cgcgggagca cgtgcgatgg tggccacctc cggcggcggc ttcgcactga tggtggaagc
      661 cctttccctt gcgggaatgt tggagacgcc ggtggtgatc gccttgggga tgcgtcccgg
      721 ccccgccacg gggatgccta cgcgcaccgg acaggaagaa ttggaattcg ccctctccgc
      781 cgggcacggg gaatttcccc gcctggtcct cgcccccggt tcggtggaag aggcgtacgc
      841 cctcgcatac cacgccatgg aaatggcgga agcgcaccag gcgccgtgca taatccttac
      901 cgatcagtac ctcgcggaca cggtcgtgga cgtcgacccg gaggccctcg cagtgctccc
      961 ggtccatcga cggatcgtcc gcgggaacga cgtaccgcgc aacccggagg ggaggtacct
     1021 ccggtacgcc atcacggcga gcggcgtatc gccgatggcg gttccggggg agccggggat
     1081 caccgtcgtg gccgacagcg acgagcacgg cgaggacggc cacctgacgg aagaccatgg
     1141 catccgcgtc cgtatggtgg agaagcgggc gcgaaaagag cagggcctcg aggcaacggg
     1201 gcttcccccg ctgctcgccg ggccgccgga cggggaagcg gcgctcgtcg gattcggctc
     1261 tacgaagggc gtgatcgccg aggcgagaga gatcctcgcc cgcgaggggg tgtctgttgc
     1321 cgcggtgcac ctgcggcagg tctggccgtt ccccgccgga gcggtcgagg agatcctctc
     1381 ccgttatggg acgacgctga ccgtggaaaa caaccgtcgc ggtcaactgg cacggctcat
     1441 ccggagggag acggggagga cggtttccgg gacagtgtcg cgcttcgacg ggcttccgtt
     1501 tgcgcccgat gaagttgccc gggaggtcaa ggagaggata tgggcggaac gttcgacacg
     1561 gcgatagaga gcgcctggtg ccccggctgt gggaacttct cgatcctcac cgcggtgaag
     1621 aaggccctgg agcgcacggg aaaggagaag cacgagatcg tcctggtctc ggggatcgga
     1681 caggcggcga agttgcccca ctacgtggac gtgaacgtct tcaatggcct ccacgggcgc
     1741 gctctcccgg cggcgctcgc gatcaagatg gcgaacccgt ccctcgaggt cgtggtgacc
     1801 agcggggacg gcgatatcta cggcgaaggg gggaaccact tcgtccacaa catccggcgc
     1861 aacccggaca tcgccgtctt tgtccacgac aaccaggtgt acggcctcac caaggggcaa
     1921 ccgtcgccca cgagcgatcc cggctggacc ggttcgctgc aacggtcggg cgtgattgcc
     1981 ggaccctttc ctcccctcgc cgtggcgatc gcgctcgggt gcggattcgt ggctcgggcg
     2041 ctggcttcgg acgtggatca cacggcggag ttgatgtacc aggcgatcgc gcacaaggga
     2101 ttcgccctcg tggacatcct ccagccatgc gtcaccttca acaagaagaa cacctaccag
     2161 tggtacgggg aaaatacgta cagggtgccg ccggaacatg accctgccga ccgggaacgc
     2221 gctttcgccc tgtcgctttc atggggagaa aggataccgg tgggtatcct ctaccggcgg
     2281 gagcgcccct gtttcgaaga ggtgcatccc gggatagggg atccaccgtt acacgcccgt
     2341 gagactccgg aggaggcggt ccggtcgatg atcgggaagc gcgtactcat ccttccgtaa
     2401 atatgcgcta ggacttgggg gtgtactcca cataggtcgg atcgtacaac tgcgctttca
     2461 catgcttcat caggtcgtcc ggacgatgca ttttcgtcag gccgcgccgg aaggccactt
     2521 ccgcgacggc cacggcgatc gagacggaca cttccctgat ccggtcaagc cgggggtaaa
     2581 tccgtcccat cgcatattcg tcctccgtga ccatgcctga aagtgtcttc gcggccgcga
     2641 agaacatctc ctccgtgacg cgcgtagcct cgctggcgat tacgcccagc cccacgccgg
     2701 ggaagatgta cgagttgttg ctctgccccg ggacgaaggt cttccccttg taagtcaccg
     2761 gctcgaacgg actgccactc gcgtataccg cacgcccctc cgaccacgta tatgcgtcct
     2821 cggccgtgca ctccgacttc gaggtcggat tcgagagggc gaagatgatc gggcgctggt
     2881 tcatgcgcgc catcgcctcg acgatcggcc tggtgaacat tcccggcgcg ccggaaacac
     2941 cgatgatggc cgtcggattg atagcttcga cggcctgccg gaaatcgccg atgaacgcat
     3001 ggtcgtgcgc aaaaggcaga tggtggggag caaggtccgt gcggctcttg acgatgagcc
     3061 cattgatatc cacgtaccag cacctgcgtt tcgcttcggt ttccggcatc ccctccgcca
     3121 tgagccccgt aacgatcagg tcgccgatgc cgatgccggc ctcccccgcg ccgaggaaca
     3181 ggaatttatg gtccgtcagc ctgttccccg tgatcctcag ggcggaatag agccccgcca
     3241 gcgcgacgct cccggtgccc tggatgtcgt cgtcgaacat gcaggcgcga tccttgtact
     3301 tgttcaggag acgaaaggcg ttcgcgttgc cgaagtcctc gagctggatg caggccctcg
     3361 ggaaaatcgc cctcacggca aggaagaatt cctccacgag ttcgtcgtac gcttcgcccc
     3421 gcaggcgcgg cgaaggatga ccgatgtaga gcgggtcgtt cagcagcgtc ttgttgttcg
     3481 tgccgcaatc cagcatgacg ggcaagcatt gcgtgggatg gatgcccgcg caggcggtgt
     3541 acagggagag cttgcccacc gggatcccca ttccgttcgc gccgaggtcg cccaggccga
     3601 ggatccgctc cccgtccgtg acgacgatga tgcggatatc gtggtacggc cagttggtca
     3661 ggatctcccg gaagcggccc tggttcttga aggaaagata cagtccccgc gggcggcgga
     3721 aaatatgccc gaactctgtg cacccctggc ctaccgtcgg ggtgtagatg atgggcatca
     3781 tctcctcgat gttgtccatg accacccggt agaagagggt ctcctggcgg tcctggagcg
     3841 agaccaggta gatgaacttc tcgatgtcgc tcggcttgcg gtggatgttt tccagcacgc
     3901 gcgacacctg ctgctccaac gtggacacgc gcggcgggag cagcccgcgc aggcccagag
     3961 cgtcacgctc cttttccgtg aaagcggttc ccttgttgat gtgcggatca tgcaacaggt
     4021 cgactccccg tttcccgttc acgtgcagat gaaggtggaa accgtacgtc atgtgttccc
     4081 tcctgccaca cacggaattc ccgtgtcgtt tcgggttgat ttacccacgg ccgtgaagct
     4141 ctccaatgag gctgtacagg cggagccctt ccacttcaaa acagatccca ctgcaatcat
     4201 gcgccttctc gagttcctga tccatggttg gtatcatgcc gaaaaaggca attgtttgca
     4261 tggggtttgc gatccctgtg gtttgcgttg catccaggca tgcgattcgt ccacaccgac
     4321 aagccgtggg cgggcccagg ggagcggcac tcgcggattc cgcgaggatc acggtacggg
     4381 cgacgggcct ggaccatcga gagtgaggca ggcgcgcacg ttctccacca aggcgggtat
     4441 atccgtttcc gtctttccgc aaaccgtaag cggcgggaat acccggaccg aaatcactcc
     4501 tttatggaag tgccccgatc cgccgggcgg acagaccttc cgggcgcctt cgatccggac
     4561 agggacgatc gggaggcccg tctggatcgc aaggtgaaag cctcccttct tcagcggcag
     4621 gagctgcccg tcctggctga ccccgccttc cgggaagatc aggaaggagt tgttccccgc
     4681 gagccccgag atcgcccctg caatggaggc cctggcctga tccgtgttct ccctgtccac
     4741 catgatctgc ccgagggaca gcagcgccca accgaataca gggatccggg caagctcctt
     4801 cttgaatacg aagtggaacg gttgggggat ggcgatcgcc atcgcaaggg ggtccgcgat
     4861 ggaggtgtgg tttggcatga acacgtacga acgttgcgga tcgatccgtt cgaacccgag
     4921 gcggacgact cgcacccgga acgcggtgta taagaccttg gagtagaatt tcgcaagcgg
     4981 gcggaaaaga ctccctttac cggtcaatcc cttccggaag aaacccagaa aaatgaaggg
     5041 aggcaagaga atcaccgtga ccacgccgac gaagatccac tggaggaagg tcgtcatttt
     5101 caatgggcag gattcctcgg gtcagcacgg aatttccttc gcgaaacggg tggaggtaca
     5161 ggggaaccgg acgtccctga aatccgatcc tcactgcaac ttccggatcc gcacctcggt
     5221 ctccttccgg tcccgcagga gttgaacga
//
LOCUS       DASVJL010000431         3364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1482525, whole genome shotgun sequence.
ACCESSION   DASVJL010000431 DASVJL010000000
VERSION     DASVJL010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482525"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VF357_09060"
     CDS             <1..1177
                     /locus_tag="VF357_09060"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX7524340.1"
                     /translation="AKAYIIASVRTAVGKAYKGSLKDTRPDDLGAAAIRGALDRVPNL
                     DPARIDDVILGCAMPEGEQGMNVARICALKAGVPDSVPAMTINRFCSSGLQSIAMAAS
                     LILAGSADVVVAGGTESMTMVPMGGNKPSFNPDLIDNRPEVYLPMGLTAEMVARRYKV
                     TREDQDQFAYHSHRKALAAIRDGKFREEIVPVSTVLFQQKDGGSLLRKEIVYDTDDGP
                     RADTTVEALSKLKPAFDAKGTVTAGNSSQMSDGAAAAIVVSEKVLKELGVEPMARFLG
                     FSVAGVAPEVMGIGPIAAIPKLMKRLRMKLNRVDLVELNEAFAAQSLPVIRELSLDPD
                     KVNVNGGAIALGHPLGCTGAKLTATLLYEMKRRNATLGLVSMCIGGGMGAAGLFERA"
     gene            1287..2585
                     /locus_tag="VF357_09065"
     CDS             1287..2585
                     /locus_tag="VF357_09065"
                     /inference="COORDINATES: protein motif:HMM:NF006096.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine synthase"
                     /protein_id="HEX7524341.1"
                     /translation="MSSSLKPETIALHGGQKPDPTTNARAVPIYQTTSYVFNDTAHAA
                     RLFGLQEFGNIYTRIMNPTTDVFEQRVAQLEGGSGALAVASGQAAETLALLNIARAGD
                     EILSSASLYGGTYNLFHYTFPKMGIEVKFVDPKDPGSFRKALTKKTKAVFAESVGNPK
                     LDTLDIAAVSKVAHDAGIPLVVDNTMPSPFLLRPFDHGADIIIHSATKFIGGHGTSIG
                     GIVVDSGKFDWGNGNFPEFTAPDPSYHGLKYWEVFGNFPGLGNVAFIIKLRVQLLRDL
                     GPALSPFNAFQFLLGLETLHLRMERHSANALAVGKFLEKHPNVSWVNYPGLPSHPSHA
                     LAKKYHHRGLYGAILGFGIKGGVAAGKKFIESLKLFSHLANIGDAKSLVIHPASTTHQ
                     QLTPAEQHATGVTDDFVRLSVGIENVDDIIEDLDQALRKS"
     gene            2720..>3364
                     /locus_tag="VF357_09070"
     CDS             2720..>3364
                     /locus_tag="VF357_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7524342.1"
                     /translation="MTTGEKILHGGGYLIENAIAGTVFTPEDFSEEQLQLADTTDQFL
                     REKVLPHVEKLENHDFDLMVKLLRQFGELGLLMIDAPEEYGGLDLPKTTSMLAAEKAS
                     IYGGFSVAYTAHSGIGTLPLVYYGTKEQKERYLGKIISGEWMAAYCLTEPDSGSDALG
                     AKATATLTPDGKHYLLNGTKQFITNGSFADLYTVFAKIDRQQFTAFLVERTFEGV"
BASE COUNT          644 a         1079 c         1084 g          557 t
ORIGIN      
        1 ggcgaaggca tatatcatcg cgtcggtgcg cacggcggtg ggcaaggcgt acaagggaag
       61 cctgaaggac acccgcccgg acgacctcgg cgcggcggcg atccgcgggg cgctggaccg
      121 tgtgccgaac ctcgacccgg cacggatcga cgacgtgatc ctcggctgcg cgatgcccga
      181 gggggagcag gggatgaacg tcgcccgcat ctgcgccctg aaggcggggg tgcccgacag
      241 cgtccccgcg atgacgatca accggttctg ctcctcgggc ctgcagtcga tcgcgatggc
      301 ggcttcgctg atcctcgcgg ggtccgccga cgtggtcgtg gccggcggca ccgagtcgat
      361 gacgatggtc cccatgggcg gcaacaagcc ttccttcaat ccggacctga tcgataaccg
      421 cccggaggtt tatctgccga tggggctgac ggccgagatg gtcgcccgcc ggtacaaggt
      481 gacgcgggag gaccaggacc agttcgccta ccatagccac cgcaaggcgc tcgcggcgat
      541 ccgggacggc aaattccggg aggagatcgt cccggtgagc acggtgctgt tccagcagaa
      601 ggacggcggc tcgctcctcc gcaaggagat cgtctacgac acggacgacg gtccgcgcgc
      661 cgacacgacg gtcgaggcac tgtcgaagct caagcccgcc ttcgacgcga aggggacggt
      721 gacggcgggg aactcctccc agatgagcga cggcgcggcc gccgcgatcg tcgtttcgga
      781 aaaggtcttg aaggagctcg gcgtggagcc gatggcgcgc ttcctcgggt tctccgtcgc
      841 cggcgtggca cccgaggtga tggggatcgg cccgatcgcg gcgatcccga agctcatgaa
      901 gcggctgcgg atgaagctta accgcgtcga cctggtcgag ttgaacgagg cgttcgccgc
      961 gcagtccctc cccgtgatcc gggagctgtc gctcgacccc gacaaggtga acgtgaatgg
     1021 cggcgcgatc gccctcggcc acccgctcgg gtgcaccggg gcgaagctca ccgccacgct
     1081 cctctacgag atgaagcgcc gcaacgcgac ccttggcctc gtctcgatgt gcatcggcgg
     1141 tggaatgggc gccgcggggc tcttcgaacg cgcgtaaccc ggcttgtggg ttatctgttg
     1201 acatctttcc gtcgcccgta taatctacat accctataac ttatatcgtc attaaggcgc
     1261 cagccggaac cgaaaggaga ccgacgatgt cctcttccct caagccggaa acgatcgccc
     1321 ttcacggagg gcagaaaccc gacccgacga cgaacgcacg ggcggtaccg atctaccaga
     1381 ccacctccta cgtcttcaac gacacggcgc acgcggcgag gctcttcggg ctgcaggagt
     1441 tcgggaatat ctatacccgg atcatgaacc cgacgaccga cgtgttcgag cagcgggtcg
     1501 cgcaactgga aggggggagc ggggcgctcg cggtggcttc cggccaggcg gcggagacgc
     1561 ttgccctgct gaacatcgcc cgcgcggggg acgagatcct ttcgtcggcc tccctctacg
     1621 gcgggacgta caacttgttc cactacacct tcccgaagat ggggatcgag gtgaagttcg
     1681 tcgatccgaa ggaccctggt agcttccgga aggcgctcac gaagaagacg aaggcggtct
     1741 tcgcggaaag cgtgggcaac cccaagctcg acacgctcga catcgcggcg gtctcgaagg
     1801 tcgcgcacga cgcggggatc ccgctggtgg tcgacaacac gatgccgtcc cccttcctgc
     1861 tgcgcccctt cgaccacggg gcggacatca ttatccattc cgccacaaag ttcatcggcg
     1921 gccacgggac ctcgatcggc ggcatcgtcg tcgactccgg aaagttcgac tgggggaacg
     1981 ggaactttcc cgagttcacg gcgcccgatc cgtcgtacca cgggttgaaa tactgggagg
     2041 ttttcgggaa tttcccgggc ctcgggaacg tcgcgttcat catcaagctg cgggtccagc
     2101 tgctgcgcga tctcgggccg gcgctgtcgc ccttcaacgc atttcagttc ctgctgggac
     2161 tggagacgct ccacctgcgg atggagcggc acagcgcgaa cgccctggcc gtggggaagt
     2221 tcctcgagaa gcatcccaac gtctcctggg tcaactaccc ggggctgcca agccacccct
     2281 cgcacgctct cgcgaagaag taccatcacc gcgggctgta cggcgcgatc ctcgggttcg
     2341 gcatcaaggg cggcgtcgct gcgggaaaga agttcatcga gagcctcaag cttttctccc
     2401 acctggcgaa catcggagat gcgaagagcc ttgtgatcca cccggcgtcc accactcacc
     2461 agcaactcac cccggcggag caacacgcca ccggagtcac ggacgacttc gtccgcctct
     2521 cggtcgggat cgaaaacgtc gacgatatca tcgaggacct cgatcaggcg cttcgcaaga
     2581 gctgagaggt tccatccgcc tggggggtcc gtcctccagg cggttttttc gcaatcgtac
     2641 ttgacattaa cgttaagtgg aagtatgaat tggagagggc cccgggaacc cggggaagct
     2701 cgaggaccga ggtgagccaa tgacgaccgg agagaagatt ctgcacggcg gcgggtacct
     2761 gatcgagaac gctatcgcgg ggaccgtgtt cacccccgag gatttcagcg aggagcagct
     2821 tcagctcgcc gacaccaccg accagttcct ccgggaaaag gtcctccctc acgtggaaaa
     2881 gctggaaaac cacgatttcg acctgatggt caagctcctg cgtcagttcg gggaactggg
     2941 tctcctgatg atcgacgctc ccgaggagta cgggggtctc gatctcccga aaaccacgag
     3001 catgctggcg gcggagaagg cttccatcta cggcggattc tccgtggcgt ataccgcgca
     3061 ctcggggatc ggcaccttgc ccctcgtcta ctacggaaca aaggagcaga aggagaggta
     3121 tctcgggaag atcatcagcg gcgagtggat ggccgcctac tgcctcacgg aacccgactc
     3181 cgggagcgac gccctcggcg ccaaggcgac cgccaccctc actccggacg ggaagcacta
     3241 tctcctgaac ggcaccaagc agttcatcac gaacgggagc ttcgccgacc tctacacggt
     3301 cttcgccaag atcgatcggc agcagttcac cgcgttcctg gtcgagcgca ccttcgaggg
     3361 ggtg
//
LOCUS       DASVJL010000432         7307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_824007, whole genome shotgun sequence.
ACCESSION   DASVJL010000432 DASVJL010000000
VERSION     DASVJL010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824007"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1673)
                     /locus_tag="VF357_09075"
     CDS             complement(57..1673)
                     /locus_tag="VF357_09075"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7524343.1"
                     /translation="MPYPLTLAAILQRNRLLFGRKEVVSRDFSGIHRYTYADYNVRVA
                     KLANALKRLGVRPGERVATFGWNSHRHLEAYLAVPCMGAVLHTLNIRLFEDQFSFIVN
                     HAEDAVMLIDEDLLPAVEALADRFPTVRAYVIMSDKKIVPATKLSPVYSYEALLDAET
                     GEYDWPSLLDENSTAALCYTTATTGKPKGVPYTHRGIYLHALAICTADVLGIREADAV
                     MPVVPMFHVNAWGIPFAAAWMGSKQVFPGSRPDAKALLSLIRDEKVTITAGVPTVWMG
                     VLQLMEREKYDVSSLKAIICGGSAAPKSLIEKVEKGFGVPFIHAYGMTETYPVVLVSR
                     LKSYMKGLPEAELYSYKAKQGLLVPGLEMRVVTEDGSEVPRDGKSMGELRLRGPWITE
                     EYYREPERTKETVRDGWLCTGDVVTVDAEGYVQIQDRTRDLIKSGGEWISSIDLENTI
                     MSHPAVAEAAVIGVPSEKWTERPMACVVLKPDFVGKIEGKEILDFLATRVAKWWIPDE
                     VVFVDAIPKTSVGKFAKRFLRDRFKEKRFA"
     gene            complement(1763..2479)
                     /locus_tag="VF357_09080"
     CDS             complement(1763..2479)
                     /locus_tag="VF357_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7524344.1"
                     /translation="MLLTIDHLSVFYGAVQALRDVSFTVAKGEIVTLIGANGAGKSTT
                     LRALSSVVRPHSGSIVHDGKSIAGLPSHQIARLGIAHVPEGRGVFANMSVRENLEMGA
                     YTRSSRKEIEESFGRVFRLFPRLAERAAQLAGTLSGGEQQMLAIGRGLVQRPNLLLLD
                     EPSMGLSPVLVSEIFRMIEEINKAGTTILLVEQNASMALAIADRAYVLEAGGIVLEGK
                     ASDLLEDPKVRAAYLGDGSG"
     gene            complement(2495..3277)
                     /locus_tag="VF357_09085"
     CDS             complement(2495..3277)
                     /locus_tag="VF357_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7524345.1"
                     /translation="MILEVRGLTRHFGGIKALDGVDFHLRRGELTGIIGPNGSGKTTL
                     FNVITGNYRPDRGAVSLEGEEITGLPPAEIARRGISRTFQNIRLFRDLTVVDNVRIAR
                     HPRVPYGPVAALLRTEGFYREEQRTREQTEEFLSIFSLQHRRKELAKNLSYGEQRKLE
                     IARALASRPKVLLLDEPAAGMNPSEVGTLMEFILRIRESFSLTVLLIEHQMRLVMGIC
                     ERLIVMDFGQVIARGFPAEIRRDPKVIEAYLGKRDDTAGKGN"
     gene            complement(3274..4263)
                     /locus_tag="VF357_09090"
     CDS             complement(3274..4263)
                     /locus_tag="VF357_09090"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7524346.1"
                     /translation="MTLLKNIAKAILPFVVLSGPVMFLSARGILNPYWVQIFQLACIV
                     AISALGLNLIYGFTGLFSLGHAAFYGVGAYTAALLTKLYVGAYGDEAVFATGAVFLGS
                     LLAGGFAAAFLAFLVGLPTLRLTSDYLGIATLGFGVIMKVLFDNADSFLPQLGGARGM
                     TGILRLTGVPWTVGALVVAILVIRNFVHSSYGRALIAIREDEVAAAAIGIDTFRYKVI
                     GFTAGCAFAGVAGGLYAHLYTFLHPSTFDFLKSFDVLMIVVLGGLGNLTGTLVASFAW
                     IFLLEGMRIALPPEYIEFRWVLIPLLLIVTMLVRPRGLFGLREFPLLRSRVYR"
     gene            complement(4274..5134)
                     /locus_tag="VF357_09095"
     CDS             complement(4274..5134)
                     /locus_tag="VF357_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7524347.1"
                     /translation="MNGLQLGFVYALIALGYTMVYGIVRLINFAHGDVFMVGAFLAFY
                     AVEKLHMPLPAVFALAAGGSAFLAVVIERVAYRPLRNAPKIAALITAIGVSLFLEYFT
                     ALHQVFGPNYYAFPRPFQVVSFEIGGVTVNGIQGIILLATLASLGALQYVVYRTKLGR
                     GMRAVSHDIVTAGLMGVNVNAVISFTFGLGAALAGVGGVLYGIAYPQINTFMGVMPGL
                     KAFTAAVLGGIGSIPGAVLGALIMGQSETMTTAYLSSTYRDGIAFVLLIAVLLWKPTG
                     ILGKSGTEKV"
     gene            complement(5349..6485)
                     /locus_tag="VF357_09100"
     CDS             complement(5349..6485)
                     /locus_tag="VF357_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7524348.1"
                     /translation="MKRATLLLAFLFPALVTIVPSHANEIKIGLITPLSGDVKTYGES
                     VRNSFLIAVEEANARGGVAGMKITYVIQDDKNDPTEAANVANLLVNQYRVKAIVGAVT
                     SKATIPVSDIIQAAKIPTLTPTATNPKVTVADGKRKDYMFRSCFIDPFQGAVMAKFSR
                     ETLKGKSAAVLFDASNDYSKGIAEVFRDAFGKQGGKVAAFESYGKDDVDFSALLTKVK
                     ASGADILFLPDYYNKVGLIAKQVQEKGLKVRLVGPDGWDSPDLVKIAGSAIEGGYFSN
                     HYSPDDRRPEVAAWVKKYKDKHGQVPDALGTLAYDGTNLLLEAIRKAGSDDPRKIRDA
                     LASIRDFHGVTGKSTLDRNGDSIKSAAIVKIEGGRQKFVKMVNP"
     gene            complement(6588..>7307)
                     /locus_tag="VF357_09105"
     CDS             complement(6588..>7307)
                     /locus_tag="VF357_09105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7524349.1"
                     /translation="EVKTVVVEYRHGDVVLEGYLAYDDSIQGKRPGVLVVHEWNGHNP
                     YVRMRAEQLARLGYVAFALDMYGKGVRAKDEKEAAALAEIFKGDRKLMRARAAAGLDV
                     LRTRPETDPARLAAIGYCFGGTTVLELARSGADLVSVVSFHGGLDTPTPGDARNIKGK
                     VLVLHGGDDPYVPPKQVEAFQEEMRKGGVALHFVSYGGAVHGFTNPESGSDNSKGAAY
                     NERADRLSWEEMKTFLAETLK"
BASE COUNT         1328 a         2436 c         2229 g         1314 t
ORIGIN      
        1 cgtcgtagcg caccctctcc ccgagcaggc aggcgctgac gaggatcctc accagctcag
       61 gcaaaccgct tctccttgaa ccggtcccgc aggaaccgct tcgcgaactt ccccaccgac
      121 gtcttcggga tcgcgtcgac gaacaccacc tcgtcgggga tccaccactt cgccacgcgc
      181 gttgcgagga aatcgaggat ctccttcccc tcgatcttcc cgacaaagtc gggcttcagc
      241 acgacgcacg ccatgggacg ctcggtccac ttttccgacg ggacgccgat caccgccgcc
      301 tcagccaccg cgggatggga catgatcgtg ttctcgagat cgatggagga gatccactcc
      361 ccgccgctct tgatgagatc gcgcgtgcgg tcctgaatct gcacgtaccc ctcggcgtcc
      421 acggtcacaa catcgccggt gcacagccac ccgtcccgga ccgtctcctt cgtccgctcc
      481 ggctcgcggt aatactcttc ggtgatccac gggcctcgga ggcgtagctc tcccatcgac
      541 ttcccgtcgc ggggcacctc ggagccgtcc tcggtcacca cccgcatttc aagccccggc
      601 acgagcaatc cctgtttcgc cttgtacgaa tagagctccg cctccggaag ccccttcatg
      661 taggacttca gccgggatac cagcaccacc ggatacgtct ccgtcatccc gtaggcgtgg
      721 atgaagggga cgccgaatcc cttttccacc ttttcgatga gggacttcgg ggcggcggac
      781 cccccgcaga tgatcgcctt caggctcgag acgtcgtact tctcccgctc catcagttgc
      841 agaaccccca tccagacggt gggcaccccc gcggtgatcg tcaccttctc gtcccggatc
      901 agggaaagga gcgccttcgc gtccggccgg gagcccggga acacctgctt gctccccatc
      961 cacgccgcgg cgaacgggat cccccaggcg ttgacgtgga acatcggcac gaccggcatt
     1021 acggcgtccg cctcccggat ccccagaacg tcggccgtgc agatcgcaag ggcgtgcaga
     1081 tagatccccc ggtgggtgta ggggactcct ttcggttttc ccgtggtggc ggtggtgtag
     1141 cagagcgccg ccgtcgaatt ctcgtcgagt agcgaaggcc agtcgtactc ccccgtctcg
     1201 gcgtcgagca gcgcctcgta cgagtagacc ggggagagct ttgtggccgg aacgatcttc
     1261 ttgtcgctca tgatcacata ggcgcggacg gtcggaaacc tgtccgccag cgcttcgacg
     1321 gccgggagca ggtcctcgtc gatcagcatt acggcatctt ccgcgtggtt gacgatgaag
     1381 gagaactgat cctcgaagag acggatgttc agggtgtgga gaacggcgcc catgcacggg
     1441 acggcgaggt acgcctcgag atgacggtgg ctgttccacc cgaaagtggc gacccgctcg
     1501 cccggccgca cacccagccg cttcagagcg ttggcaagct tcgcgacccg gacgttgtaa
     1561 tcggcgtagg tataccggtg gatgccggag aaatcccgac tgacgacctc tttcctgccg
     1621 aagagaagcc ggtttcgctg caggatcgcg gccaaggtca gcgggtacgg catgaagcac
     1681 ctccttgggc gggttcctcg aaaaatggaa ggcgaatgtc atttcgcccc cgaccggtct
     1741 cgatgtcaac tacggaaatg attcatcccg aaccgtcgcc caggtaagcg gcccgtacct
     1801 tcggatcctc caggagatcg gacgccttcc cttcgaggac gatccctccc gcctcgagga
     1861 cgtaggcgcg gtccgcgatg gcgagagcca tggaggcatt ctgctcgacg agaaggatcg
     1921 tcgtccccgc cttgttgatc tcctcgatca tccggaagat ctcgctcacc aggacggggg
     1981 acaatcccat ggacggctca tcgagaagga gcagattggg ccgctggacg aggccccttc
     2041 caatggccag catctgctgc tcccccccgg acagggtccc ggcgagctgg gccgctcgct
     2101 cggccagtcg cggaaagaga cggaagaccc gtccgaagct ctcctcgatc tctttccgcg
     2161 acgagcgggt gtacgccccc atctcgaggt tctcccggac gctcatgttg gcgaagacgc
     2221 cccggccttc cggaacgtgg gcgatcccca ggcgcgcgat ctggtgggac gggagacccg
     2281 ctatcgattt cccgtcgtgc acgatgctcc ccgaatgcgg acggacgacg ctggagagtg
     2341 cccgcagggt tgtgctcttc cctgccccgt ttgcaccgat gagggtgacg atctcacctt
     2401 ttgcaacggt gaaggagaca tcgcgaagcg cctgtaccgc tccgtaaaat acgcttagat
     2461 gatcaatggt taatagcaat atctaacctt ttccttaatt tcctttcccg gcggtgtcgt
     2521 cccttttccc gaggtacgct tcgatcacct tcgggtccct ccggatctcc gcgggaaaac
     2581 cccgggcgat cacctggccg aagtccatca cgatcagtcg ttcacaaatc cccatcacca
     2641 gacgcatctg gtgctcgatc agcaggacgg tcagggagaa gctctcacga atcctgagga
     2701 tgaattccat cagcgttccc acctccgaag gattcatccc cgccgcgggc tcgtccagca
     2761 ggaggacctt cgggcgcgac gcaagcgcgc gggcgatctc cagtttccgc tgctcgccgt
     2821 acgacaggtt ctttgcgagt tccttccgtc gatgctgaag ggaaaagatc gaaaggaatt
     2881 cctccgtctg ttcacgcgtc cgctgctcct cccggtagaa tccctccgtc cgaagcaggg
     2941 ccgccacggg accgtacggg acgcgcggat ggcgcgcgat ccgtacgttg tcaaccacgg
     3001 tcagatcacg gaaaagacgg atgttctgga aggtccgcga gattccccga cgggcaatct
     3061 cggccggggg aagccccgtg atctcctctc cctccaggga gacggcgccg cggtccgggc
     3121 gatagttccc ggtgatcacg ttgaaaagcg tcgtctttcc ggatccattt gggccgatga
     3181 tcccggtgag ctcgccccgc cgaaggtgga aatccacccc atccagcgcc ttgatccccc
     3241 cgaaatgccg ggtgagtccc ctgacctcga ggatcaccgg tacaccctgc ttcgcagcaa
     3301 cgggaactcc cgaagaccga acaggccccg gggccggacc agcatggtga cgatcagcag
     3361 cagggggata aggacccagc ggaactcgat gtactcggga gggagcgcga tgcgcatccc
     3421 ctcgagcagg aagatccagg cgaaggaggc caccagcgtg cccgtcaggt ttcccagtcc
     3481 cccgaggacg acgatcatca ggacgtcgaa cgacttcagg aagtcgaagg tgctcggatg
     3541 gagaaacgtg tacaggtgcg cgtacagtcc gcccgccacg ccggcgaagg cgcatccggc
     3601 ggtgaagccg atcaccttgt accggaaggt gtcgatccct attgccgccg cggcgacctc
     3661 gtcctcgcgg atggcgatga gtgcgcgccc gtaggacgag tggacgaagt tgcggatcac
     3721 gagaatcgcg acgacgagcg cgccgaccgt ccacgggaca ccggtcaacc ggaggatgcc
     3781 ggtcatcccg cgggcgcccc ccagttgagg caggaaggaa tccgcgttgt cgaagaggac
     3841 cttcatgatg accccgaagc ccagcgtggc gatgccgaga tagtccgacg tcaggcggag
     3901 agtcggcagg ccgacgagga acgcgaggaa ggccgccgcg aaccctcccg cgaggaggga
     3961 accgaggaag accgccccgg tcgcgaagac cgcctcatcc ccgtaggcgc ccacgtagag
     4021 tttcgtcagc agcgcagccg tgtacgcccc cacgccgtaa aaggcggcgt gtcccaggga
     4081 gaacagtccc gtgaacccgt agatgaggtt cagacccagc gcggagatcg cgacgatgca
     4141 cgccagttgg aaaatctgaa cccagtaagg gttcaggatc ccccgtgcgg agaggaacat
     4201 gaccggtccc gagaggacga cgaacggcag gatcgccttc gcgatatttt tcagaagggt
     4261 catcgagccc acgtcacacc ttctccgtcc cggattttcc gagaatcccg gtgggcttcc
     4321 acagcagcac ggcgatgagc agaacgaagg cgatcccgtc ccggtaggtc gaggatagat
     4381 acgccgtggt catcgtctcc gattgcccca tgatcagggc cccgaggacc gcccccggaa
     4441 tggatccgat cccgccgagg acggcggctg tgaaggcctt cagcccgggc atcaccccca
     4501 tgaaggtgtt gatctgcgga taggcgatcc cgtaaagaac tccccccacc cccgcgagcg
     4561 ccgccccgag gccgaaggtg aaggagatca cggcgttgac gttgaccccc atcaaccccg
     4621 cggtgacgat gtcatgggag accgcgcgca tcccccggcc caacttcgtc cggtagacca
     4681 cgtattgcag cgcccccagg gaagcgaggg tggcaaggag gatgatcccc tggatgccgt
     4741 tgaccgtgac cccgccgatc tcgaacgaaa ccacctggaa gggacggggg aaggcgtaat
     4801 agttcggccc gaagacttga tggagcgcgg tgaaatattc caggaacagg gaaacgccga
     4861 tggcggtgat cagcgccgcg atcttggggg cgtttcgcag cggccggtac gccacccgct
     4921 cgatgaccac cgcgaggaag gcggatcctc ccgcggcgag ggcgaatacg gcgggaaggg
     4981 gcatgtggag tttctcgacc gcgtagaacg ccaggaacgc ccccaccatg aagacgtcgc
     5041 cgtgggcgaa gttgatgagg cgcacgatcc cgtacaccat cgtgtacccc agtgcgatca
     5101 gggcatacac gaatccgagc tggaggccgt tcagcgcctg ctggaggaaa tactctacgg
     5161 tttcaccccc tagcccgcat ttctcgccag gattctactg cggcggcgga tacgaacatg
     5221 tctactgcgt tgcgctcggt cgggctcctc gacgtagtct caactacgcc tccggggccc
     5281 tcggtcgcgc gccttgtatc cacgcccatc tccaccacct cgctacgaac cctggcgaga
     5341 aatgcgggct acgggttcac catcttcaca aacttctgcc ggccgccctc gatcttcacg
     5401 atcgcggcgc tctttatgct gtccccgttc cgatcgagtg tggatttccc ggtaaccccg
     5461 tggaagtccc tgatcgaggc gagggcatcc cggatcttcc tcgggtcgtc gctccccgcc
     5521 ttccggatcg cctcgaggag cagattcgtg ccgtcatacg ccagggttcc gagggcgtcg
     5581 gggacctggc cgtgcttatc cttatacttc ttcacccacg ccgcgacttc cggccgccgg
     5641 tcgtccgggg agtagtggtt ggagaagtag ccgccctcga tcgcgctccc ggcgatcttc
     5701 accaggtcgg gggagtccca cccgtccggt ccgaccaggc ggaccttgag ccccttctcc
     5761 tggacctgct ttgcgatcag gccgaccttg ttgtagtagt ccggcaggaa gaggatgtcg
     5821 gcgcccgagg ctttcacctt cgtcaggagg gccgaaaagt cgacgtcgtc cttcccgtac
     5881 gactcgaagg ccgccacctt gccgccctgt ttcccgaacg cgtcgcggaa gacctcggcg
     5941 atccccttcg agtagtcgtt cgacgcgtca aacaggaccg cggcgctttt ccccttcagc
     6001 gtctcccggg agaattttgc catcacggcc ccctggaacg ggtcgatgaa gcaactccgg
     6061 aacatgtagt ctttccgctt cccgtccgcc accgtgacct tcgggttcgt ggccgtcggg
     6121 gtgagcgtgg ggatctttgc ggcttgtatg atgtccgaga cggggatcgt cgccttggag
     6181 gtgaccgcac cgacgatcgc cttgacacgg tactggttca ccaggaggtt cgccacgttc
     6241 gccgcctccg tggggtcgtt cttgtcgtcc tggatgacgt aggtgatctt catcccggcg
     6301 acgccgcccc tggcgttcgc ctcctcgacg gcgatcagga aggagttgcg gaccgattcg
     6361 ccgtacgtct tgacgtcgcc ggaaagcggc gtgatcaggc cgatcttgat ctcattcgcg
     6421 tgggagggga cgatcgtcac cagtgccgga aaaagaaagg cgaggagaag ggttgctcgc
     6481 ttcatcgttc cctctttcgg tacgctggag catggatatt gcggggggtg gagcgcaccc
     6541 cgggggtcat tgtacccgaa gaaccgcccc ggagtgcact tcccgattca tttgagggtt
     6601 tccgccagga aggtcttcat ctcctcccac gacaggcggt ccgcccgctc gttgtaggcg
     6661 gcccccttcg agttgtcgct ccccgactcg gggttggtga agccgtggac ggcccctccg
     6721 tacgaaacga agtgcaaggc cactcccccc ttgcgcattt cctcctggaa cgcctcgacc
     6781 tgcttcggcg ggacgtacgg gtcgtccccg ccgtggagga cgagtacctt ccccttgatg
     6841 ttgcgcgcgt ccccgggggt cggggtgtcc agccctccgt ggaagctgac caccgatacc
     6901 aggtccgccc cgctccgggc cagctcgagc accgtggtcc cgccgaaaca gtagccgatc
     6961 gcggcaagac gcgcagggtc ggtctccgga cgggtccgga gaacgtcaag cccggccgcg
     7021 gcgcgggccc gcatcagctt ccggtccccc ttgaagattt ccgcgagggc ggccgcctcc
     7081 ttctcgtcct tcgcccgcac gcctttgccg tacatgtcca acgcgaacgc gacgtacccc
     7141 agccgggcca gctgctccgc ccgcatccgg acatacgggt tgtggccgtt ccattcgtga
     7201 accacgagga ccccgggccg cttcccctgg atcgagtcgt cgtacgccag gtacccttcc
     7261 agcacgacgt ctccgtgccg atattccacc actacggttt tcacctc
//
LOCUS       DASVJL010000433         3766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1483049, whole genome shotgun sequence.
ACCESSION   DASVJL010000433 DASVJL010000000
VERSION     DASVJL010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483049"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VF357_09110"
     CDS             <1..253
                     /locus_tag="VF357_09110"
                     /inference="COORDINATES: protein motif:HMM:NF021210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HEX7524350.1"
                     /translation="VPLDTHMARLGDALGLTRRKTPDWRMAEEITASLRLACPEDPVK
                     FDYPLTRLGILGVCTRSRRGVCGNCPVAPLCSRRSRTAG"
     gene            344..1909
                     /locus_tag="VF357_09115"
     CDS             344..1909
                     /locus_tag="VF357_09115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7524351.1"
                     /translation="MAEKKMKIGEILVASEVIKEEQLTDALRSQSQLGGTLGENLIRL
                     AFLTEEELLNSLSEQLGMQHINLTKVEVPAAVQRLVKMETVRLRRLLPIGFEGKRLVV
                     GMVDPTDLSALTEVEFQSGHTTKPVILSASHFELAQSFFQTHGYGDSTLKFDAEKEAA
                     RHVRVENTLASMLTVLLSWKGQDLHLSAGAIPSIRVDNEIRRLSLPVLKPAEIEQMIY
                     AILTSEQRRYFQENLELDFAFSLHGVGRFRCNLYRQRNSVAFTARHVSEEVPTAAELG
                     IPDFLHEFGLKHQGLILITGPNGHGKSTTLAWLVDMINRDRRSNIITIEDPIEFTHRH
                     KNSNVNQREVGTDTLSFSDGLRHIFRQNPDVIVIGELRDYDSISIALTAAETGHLVLG
                     TLHSMNATAAVDRIVDAFPANQQPQIRTQLAESLLLVFSQRLLKRANGTGRVLAWEKM
                     GTSLRVRNAIREGKAHQLRGMMQANVDELVSIDWTLADMVVAGKVKYEEAVKFADNLT
                     YLNELLKVRGAFK"
     gene            1953..2753
                     /locus_tag="VF357_09120"
     CDS             1953..2753
                     /locus_tag="VF357_09120"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF005556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HEX7524352.1"
                     /translation="MIGKKIRLERIMDRNTHRTVLVPMDHGVTVGPIPGLIQIPPAAN
                     LIAEGGANAAIVHRGAAMFGHRGYGKDLGLILHLSASTTLAPDSNRKVLVATVEDALQ
                     MGADAVSIHVNLGAEDEAQMLRDFGTVSSACQHWGMPLLAMIYTRGPKIRNEYDVRYV
                     RHAARVGAEMGADIVKVPYTGSPETFTEVTQGCASSVVIAGGEKMASDEEVLRMVHDA
                     IAAGCAGASIGRNVFQHRSPASMVRAIVSIVHGGATVREALGILKAKP"
     gene            3042..>3766
                     /locus_tag="VF357_09125"
     CDS             3042..>3766
                     /locus_tag="VF357_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HEX7524353.1"
                     /translation="MSFPRLLARVVPWDKEVAVAAIESGVEALWVPDGRATFARELGR
                     VVAACAEGDLREGIDFRVTRMEGKEDEARIAGSPPDAAWVVFPRDREIIPLENLVAWG
                     RRILVVARTPADVSLYRGVLEKGVYGLVLDARDPAGMRALAAAARAKTEDVSLVSARV
                     VEVVPLGMGDRVCVDTCTWIEGSRGMLVGNGSAGMFLVCAENVANPYVLPRPFRVNAG
                     AVHSYCRVPGGRTAYLSELVAGSG"
BASE COUNT          641 a         1226 c         1252 g          647 t
ORIGIN      
        1 cgttcccctc gacacccata tggcgcgcct gggggacgct ctcggactca cccggcggaa
       61 gacgccggac tggaggatgg cggaggagat caccgcctcc ctgcgcctcg cgtgcccgga
      121 ggacccggtg aagttcgatt atcctctgac ccgtctcggc atcctcgggg tgtgcacccg
      181 ttcccggcgc ggcgtctgcg gcaattgtcc cgtggcgccc ctctgttcgc gaagaagccg
      241 gaccgcaggg tgagtcgacc ggcgggtgaa attggggttg cccccctcca tcctctgcga
      301 tagatttgat caggatgcga tgaccggtcc cggggggtgg agcatggcgg aaaagaagat
      361 gaaaatcggg gagatcctcg tggcctctga agtgatcaag gaggagcagc ttaccgacgc
      421 gctgcggagc cagagccagc tcgggggaac gctgggggag aatctcattc ggctggcgtt
      481 cctcacggag gaggagctgt tgaattccct ctccgagcaa ctggggatgc agcacatcaa
      541 cctcaccaag gtcgaggttc ccgcggcggt gcaacggctg gtcaagatgg aaaccgtccg
      601 cctgcgtcgc ctcctcccca tcggtttcga ggggaagcgc ctggtggtcg ggatggtcga
      661 ccccacggac ctttccgccc tgaccgaggt ggagttccag tcggggcaca ccacgaagcc
      721 ggtcatcctg tccgcttcgc atttcgaact ggcccagtca ttcttccaga cgcacgggta
      781 cggggattcc acgctgaagt tcgatgccga gaaggaggcg gcacgacacg tccgggtgga
      841 gaacaccctc gcctcgatgc tcacggtgct cctctcgtgg aaggggcagg acctgcacct
      901 gtccgcgggg gcgatcccct cgatccgtgt cgacaacgag atccgccgac tgagcctacc
      961 ggtcctcaag cccgcggaga tcgagcagat gatctacgcg atcctcacgt cggagcagcg
     1021 ccgatacttc caggaaaatc tcgaactcga cttcgcgttc tccctgcacg gggtcggccg
     1081 tttccggtgc aacctgtacc gccagcgcaa ctcggtcgcc ttcaccgcgc gccacgtgtc
     1141 cgaggaagtc ccgacggcgg cggagctcgg gatccccgac ttcctgcacg aattcggcct
     1201 gaaacaccaa gggctgatcc tcattaccgg cccgaacggc cacgggaagt cgaccaccct
     1261 tgcgtggctc gtcgacatga tcaaccggga tcggcggtcc aacatcatca cgatcgagga
     1321 tcccatcgag ttcactcacc ggcacaagaa ctccaatgtg aaccagcggg aggtggggac
     1381 cgacaccctc tctttctcgg acggtcttcg gcacatcttc cggcaaaatc cggacgtcat
     1441 cgtgatcggg gagctccggg attacgatag catttccatc gccctcaccg ccgcggagac
     1501 gggacacctg gtcctgggaa cgctccactc catgaacgcc acggcagccg tggatcggat
     1561 cgtggacgct ttcccggcga accagcagcc tcagatccgc acgcagttgg ccgagtcgct
     1621 gctcctcgtc ttctcccagc gcctgctcaa gcgcgccaac ggtacggggc gcgtgctcgc
     1681 gtgggaaaag atgggaacct cccttcgtgt ccgcaacgcc atccgggagg ggaaggccca
     1741 ccagttgcgg gggatgatgc aggccaacgt cgacgagctg gtgtcgatcg actggacgct
     1801 ggcggacatg gtggttgccg ggaaggtgaa gtacgaggag gcggtgaaat tcgccgacaa
     1861 cctgacctat ctgaacgaac tcctcaaggt ccgaggagcg ttcaagtagg ggagccgccc
     1921 gcggggatcc ccgggccgga ggcgcacgtc cgatgatcgg aaagaagatc cgcctggagc
     1981 ggatcatgga ccggaacacc cacaggaccg tgctcgttcc gatggaccac ggcgtgaccg
     2041 tcggcccgat ccccggactc atccagatcc ccccggcggc gaacctcatc gccgagggtg
     2101 gggcgaacgc ggcgatcgtg caccgggggg ccgccatgtt cggtcaccgc ggctacggga
     2161 aagacctcgg cctcatcctg catctatccg ccagcaccac cctcgccccc gactccaacc
     2221 ggaaggtcct ggtggccacc gtcgaggacg ctctgcagat gggggccgac gccgtctcca
     2281 tccacgtgaa tctgggcgcc gaggacgagg cgcagatgct gcgcgacttc ggcacggtat
     2341 cgagcgcctg tcagcactgg ggaatgccgc tcctcgccat gatctacacg cgcggcccga
     2401 agatccggaa cgagtacgac gtcagatacg tccgccacgc cgcgcgtgta ggggcggaaa
     2461 tgggagccga catcgtcaag gttccctaca cgggatcccc cgagacgttc accgaggtca
     2521 cgcaggggtg cgcctcctcc gtggtgatcg cgggagggga gaagatggcg agcgacgagg
     2581 aagttctccg gatggtccac gacgccatcg cggcggggtg cgcgggggcc tcgatcggga
     2641 ggaacgtgtt ccagcaccgc tccccggcgt ccatggtgcg cgccatcgtc tcgatcgtcc
     2701 acggtggcgc caccgtgcgg gaggcgctcg ggatcctcaa ggcgaaacct tagtggtgcg
     2761 tttcataaat acgctatcat ttcaccgttg gggagtagga ctccgggctc gcgggggggc
     2821 ttcctctccg ctacggctcg tcgtccctcc ctggattcct cgcctgcgct ttcgctcgtt
     2881 ctcgacgtcc ctgtctccac ttcgctccag acgccgcttc gaggaacccc cccgctgcgc
     2941 cctgcgtccc agtacctccg gaggcgcatc ggagcggaaa tatcacgcgg agatcaaggc
     3001 gcctccgttt acggtaccgt attaatgaac tggggcgcct tgtgagcttt cctcggctcc
     3061 tcgcccgggt cgtcccttgg gacaaggaag tggccgtcgc cgccatcgaa tcgggggtgg
     3121 aggcgctttg ggtgccggac ggccgcgcca cgttcgcccg ggaactgggg cgcgtcgtgg
     3181 cggcatgcgc cgagggggac ctgcgggagg ggatcgactt ccgggtgacc cggatggagg
     3241 ggaaggaaga cgaagcgcgg atcgccggct cccccccgga cgcggcctgg gtcgtctttc
     3301 cgcgggaccg ggagatcatc cccctcgaga accttgtggc ttggggacgc aggatcctgg
     3361 tcgtggcccg tacgcccgcc gatgtgtcgc tctaccgggg tgtcctggaa aagggggttt
     3421 acgggctggt cctggatgcc cgggatcccg cggggatgcg agcgctcgcc gccgccgccc
     3481 gggcgaagac ggaggacgtt tcgctggttt ccgcccgggt ggtcgaggtc gttcctctcg
     3541 ggatgggcga ccgtgtctgc gtggacactt gcacctggat cgaggggagc cgcgggatgc
     3601 tggtcggcaa cgggagcgcg gggatgttcc tcgtgtgcgc cgaaaacgta gcgaacccgt
     3661 atgtccttcc acgtccgttc cgcgtcaacg cgggcgcggt ccactcctac tgccgcgtcc
     3721 ccggaggccg taccgcctac ctttccgaat tggtcgccgg ttcggg
//
LOCUS       DASVJL010000434         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1858199, whole genome shotgun sequence.
ACCESSION   DASVJL010000434 DASVJL010000000
VERSION     DASVJL010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858199"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..>1598)
                     /locus_tag="VF357_09130"
     CDS             complement(214..>1598)
                     /locus_tag="VF357_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX7524354.1"
                     /translation="LGQHEDIPALCEKHEVDEIIIAIPSASPSHLRHLLDHCKDVKAR
                     ARILPGVGDLIDGKVSIRALRNVDLEDLLGRDPVALDAELLHRHITGRTVMVTGAAGS
                     IGSELCRQIARRSPGRLILFEIAESPLFYLEIEMREKFPALGLVPVIGDVRDRGRVEE
                     IVRAHRPAIIYHAAAYKHVPVMEMHPVEAVKTNVLGTRIVAEAAAKFGVERFVLVSTD
                     KAVRPTNVMGATKRLAEIVIHNIANRQGPTVFVAVRFGNVLGSVGSVIPIFRRQLMTT
                     GKLTVTHKDASRYFMLIPEAAGLILQAGAMGEGGEVFVLDMGEPVKIVDLAKNLIRLS
                     GKELGVDAEIVFTGLRPGEKLHEELIVEGEDVSGTVHPKVMKLIGNERMSPSWSMRLE
                     DLIVCAITGARCDVIEKLDALVKGYRPNYEFHGVAVPPPAANPATVSLVEASLESSSE
                     MIPPSPKSVH"
BASE COUNT          275 a          551 c          487 g          285 t
ORIGIN      
        1 cgaaccttca tggccgtcgc ttcccggatc atcgtttcac ctcaattgat atggttatat
       61 gaattatagc gacatggggc gacaagtcaa ccggcgaact cccccaatcc ggggacacag
      121 atcgcatcga tgccgacgcc acgtctgtcc ccgtcctggg ggagttcgca ggaacgtccc
      181 cgttccgcga ttatgaaggt gattcggggt gggttaatgg acggacttgg gagagggagg
      241 gatcatttcg gaggaggatt cgagggaggc ttcgactaga gagacggttg cggggttggc
      301 ggcaggcggg gggacagcga ctccgtggaa ttcgtagttc gggcggtacc ctttcaccag
      361 ggcatcgagc ttctcgatga cgtcacaacg ggccccggtg atggcgcaca cgatcaaatc
      421 ctccagccgc atcgaccagg agggggacat ccgctcgttg ccgatcaact tcatcacctt
      481 cgggtggacg gtgccgctca cgtcctcgcc ctcgacgatc agctcctcgt gcagcttctc
      541 gcccgggcgc agccccgtga agacgatctc ggcatccacg cccagctcct tcccgctcag
      601 gcggatcagg ttcttcgcca ggtcgacgat cttcaccggc tcgcccatgt cgaggacgaa
      661 cacctcgcca ccctccccca tcgcgcccgc ctgaaggatc agcccagccg cctcggggat
      721 cagcatgaag tagcgcgacg catccttgtg ggtgacggtc agcttccccg tcgtcatcaa
      781 ctgcctgcgg aagatcggga tcacgctccc cacgctgcca aggacgttgc cgaaccggac
      841 ggcgacgaac accgtcggcc cctgccggtt ggcgatgttg tggatcacga tctccgccag
      901 ccgcttcgtc gcccccatca cgttcgtcgg gcgcaccgcc ttgtccgtgg agacgagaac
      961 gaatcgctcc acgccgaact tcgcggccgc ctccgccacg atccgggtcc ccagcacgtt
     1021 cgtcttcacc gcctccaccg ggtgcatctc catcaccggc acgtgcttgt acgccgccgc
     1081 gtggtagatg atcgcgggac gatgggcgcg gacgatctcc tcgacccttc cccggtcgcg
     1141 cacgtctccg atcacgggga ccagacccag ggccgggaac ttctcccgca tctcgatctc
     1201 gaggtagaag agcgggctct ccgcgatctc gaacaggatc agccgccccg gcgaccgccg
     1261 cgcgatctgc cggcacagct ccgagccgat cgaccccgcc gcaccggtca ccatcaccgt
     1321 gcgccccgtg atgtggcggt gcagcagttc cgcatccagc gccaccggat cccgcccgag
     1381 caggtcctcg aggtccacgt tccgcagagc gcggatgctc accttgccgt cgatcaggtc
     1441 gccgacgccg ggcaggatcc gcgccctggc cttcacgtcc ttgcaatgat caaggaggtg
     1501 gcgcaggtgg gacggggagg ccgacgggat ggcgatgatg atctcgtcga cctcgtgctt
     1561 ttcgcagagc gccgggatat cctcgtgctg tcccagaa
//
LOCUS       DASVJL010000435         5030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_169541, whole genome shotgun sequence.
ACCESSION   DASVJL010000435 DASVJL010000000
VERSION     DASVJL010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169541"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VF357_09135"
     CDS             <1..429
                     /locus_tag="VF357_09135"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX7524355.1"
                     /translation="MGKFGADAVQMATRFVLTKECDVSDRFKQAYLNARKEDVVLIKS
                     PVGLPGRAIRNPFLERLFRGGDVYDGECRRGCLKNCSHNFCIIDRLEMSRNGETEEGL
                     VFTGENVWRIKDIPSVKELIDRLVAEAESVYAPASAAAPA"
     gene            complement(483..1790)
                     /locus_tag="VF357_09140"
     CDS             complement(483..1790)
                     /locus_tag="VF357_09140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7524356.1"
                     /translation="MFSPVGVDDRVPGAPDSLYNDAITKGKGRSVPHRPSINPPPANG
                     ATVPTPILVACVLTLVLYLGAYMRLPLVPLFARGLGASTVDVGMINACFMLAAAALAL
                     PLGLMSDRLGRKRLILAGMGISCLTSLFLLVARTPLHVGLIYLFSGVGLACFSPAMMS
                     HVGDSSPPNFLGRAYGWYTSALYLGMALGPGFGGAVATKGFGAAFAVSAAIIGAGGIV
                     AAFRVGDPPPPAPRPSGGGHLRSDFGEIIRNRAVLGCWVATFFSTYAWGSLFAFFPLY
                     ANELGISIPQTGLIFTTQAGVNALCRIPIGHFQDRSGNRRSFILWGNALFGLCIALTG
                     TARGPLPLYLLFAGVGATMAATFTAVGAVLSESVATRVRGLAMGGYNTCIYGGFMASA
                     ATLGFVIQRMGYTAGFAVAGLSCVVATAGVAVLLSPKPSETLH"
     gene            1917..2348
                     /locus_tag="VF357_09145"
     CDS             1917..2348
                     /locus_tag="VF357_09145"
                     /inference="COORDINATES: protein motif:HMM:NF017277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX7524357.1"
                     /translation="MDKKALLELTTDIVSAHASVNEMGKEELLEELQSVFQKLMSLAG
                     SEGGEGAEVPGELKPAVPVNKAFGADKVVCLVCGKGFTTLKKHIAVSHQMTPKEYRKA
                     FGVPSKTPLVARKYSEAKRKIAQDKGLATKLAEGRRKKAEK"
     gene            2516..3094
                     /locus_tag="VF357_09150"
     CDS             2516..3094
                     /locus_tag="VF357_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX7524358.1"
                     /translation="MPYVAKDFGTLVGMDGFSETLLKNHFTLYQGYVTNTNKLSDLLG
                     GMLAEGKTATPEYAELKRRFGWEWNGMRLHEFYFENLGGKGALDPASKLGKAVASEFG
                     GVEKWEADFRAAGAMRGIGWVVLYQDKLGGRLFNQWVNEHDVGNPAGAVPLFVMDVFE
                     HSFMVDYGLKRADYIAAFFRNVNWKAAEARLS"
     gene            3201..>5030
                     /locus_tag="VF357_09155"
     CDS             3201..>5030
                     /locus_tag="VF357_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7524359.1"
                     /translation="MDYYAQGSLVAAVVALAIAVYLRVFARGDAEARAFSDLSLVIFL
                     WCFPEYLWKTLPSTFWHKVSLAGVMFIPPFVLRYCGAAVRSRPRWLPAAFVAGVWASV
                     AFAVTIFYDPALNSPLWNVAALLFVYPYLGAGLYVIVRYGFRNSPSAVERKKYQFLVV
                     GGGIASLVAPLNFLPGTGVDFPPLAAFAVLVFFYFMATGILHLHFFELPDIVGRGVVL
                     VIQIFAFAVVFGMMEVVSGRKFWFPLAGVFLISFFLLSFYPFLMRKLGGISADLLVRQ
                     SRRVQSVLSDFARKLPESTVLAEIGSTVEEALAQVPFVERASLWVRPEGGTDEGIGTL
                     PVRPDEYLRAFTRFTKRYPALRIMEHGGSKAADVAIWDDSFDASIPLFLRGVLVAVVL
                     FHWRGKRPSFREMDLLIPFLDGIALAVENIRQQQRIQRREHFATVGELAAGLAHEVRT
                     PAGVIKGAAQFLSREARPKDREFLDIIVEEADRLNGVVTEFLEYARPTERKPQTISLK
                     EPVEKAIALLLRERGERMGTVGRHVLTQEPVPRVNADPAEIERVVYNLLTNAIEAMPG
                     TGDLTVRVTSEGGEARIAVEDTGAGIPEADRPQLFRPFFTTRER"
BASE COUNT          892 a         1535 c         1760 g          843 t
ORIGIN      
        1 atggggaagt tcggcgccga cgcggtgcag atggccacgc gattcgtcct cacgaaggag
       61 tgcgacgtgt ccgaccgctt caaacaggcg tacttgaacg cccgcaagga ggacgtggtt
      121 ctgatcaaat cccccgtggg gctgcccggg cgcgcgatcc gcaacccgtt cctggagcgc
      181 ttattccgcg gcggagacgt ctacgacggg gaatgccgca gggggtgcct gaagaattgc
      241 agccataatt tctgcatcat cgaccgcctc gaaatgtcgc gcaacggtga gacggaggaa
      301 gggctggtct tcacggggga gaacgtctgg aggatcaagg acatcccgag cgtgaaggag
      361 ttgatcgacc gcctcgtcgc cgaggccgag agcgtctacg cccccgcgtc cgccgccgct
      421 ccggcctgaa agaccctccc ccttgggacg atgttcgtaa ccgccgggag cgcccggtca
      481 gcctagtgca gcgtctcgct cggcttgggc gacaatagaa cggccactcc ggccgtcgcg
      541 acgacgcaag acagtccggc gaccgcgaac cccgccgtgt accccatccg ctggatcacg
      601 aacccgagcg ttgccgcgga ggccatgaac ccgccgtaga tgcacgtgtt gtaccccccc
      661 atcgccaatc cccggacgcg ggtggcgacc gattccgaca ggacggcgcc gaccgccgtg
      721 aacgtcgccg ccatcgtggc gccgactccg gcgaagagaa ggtagagagg cagcggccct
      781 cgcgccgttc cggtgagggc gatgcagagc ccgaacaggg cgttccccca gaggatgaaa
      841 gaacgccggt tccccgaccg gtcctggaaa tggccgatcg ggatgcggca gagcgcgttg
      901 acgcccgcct gggtcgtgaa gatgaggccg gtctgtggga tcgagatccc gagttcattg
      961 gcgtaaagag ggaagaaggc gaacagcgac ccccacgcgt aggtcgagaa gaaggtggcg
     1021 acccagcaac cgaggacggc ccgattgcgg atgatctcgc cgaagtccga gcggagatgc
     1081 ccgcctccgg agggccgtgg ggccggggga ggcgggtccc cgacgcggaa ggccgccacg
     1141 atccctcccg cgccgatgat cgcggcggag accgcgaagg cggccccgaa ccctttcgtc
     1201 gcaacggctc cgccgaagcc cggccccagc gccatcccga ggtatagggc ggaggtgtac
     1261 cacccgtacg cccgcccgag gaagttcggc gggctggaat cccccacgtg ggacatcatc
     1321 gcgggggaga agcaggcaag gcccaccccg gagaaaaggt agatcaggcc gacgtgcagg
     1381 ggagtccggg ccacgagcag aaagagggag gtaaggcagg agatccccat cccggcaagg
     1441 atgaggcgct tgcgcccgag acggtcggac atgaggccca gcgggagggc gagggcggcg
     1501 gctgccagca tgaagcaggc gttgatcatt ccgacgtcga cggtggaggc tcccagtccc
     1561 cgcgcgaaca acgggaccag ggggaggcgc atgtaggctc cgagatacag tacgagcgtc
     1621 agtacgcacg cgacgagaat gggcgtcggg accgttgcgc cgttggcggg tggcgggttt
     1681 attgaaggac gatgggggac ggatcggccc tttcctttcg taattgcgtc attgtacaag
     1741 gaatcgggcg ccccaggaac tctgtcatcg actccgacag gggaaaacat gaaatgaacc
     1801 gcatttctgt cggagtgcgg ttcgtatatt ccggaaaata attcgatccc gtattgacat
     1861 cacgggatca attgaagaaa ataaaaacac caagacggaa atccctggag ggaatcatgg
     1921 acaagaaggc gctgttggag cttacgacgg acattgtatc ggctcacgct tcggtaaatg
     1981 agatggggaa ggaagaactt cttgaggaat tgcagtcggt gttccaaaag ttaatgagcc
     2041 ttgccggatc cgagggtggg gaaggggcgg aagtccccgg ggagttaaag ccggccgttc
     2101 cggtgaacaa ggcgtttggc gccgacaagg tcgtttgcct ggtttgcggc aaagggttca
     2161 ccacgctgaa gaagcacatc gccgtcagcc accagatgac gccgaaagag taccggaagg
     2221 cgttcggcgt cccgtcgaag acccccctcg tcgcccggaa atattccgag gcgaagagga
     2281 agatcgccca ggacaagggg cttgcgacga aactggcgga aggccgaagg aagaaagcgg
     2341 agaagtaacc aggagatcgc cgcggccgcg agcgccgcga tctggtcgca gggagggccc
     2401 gccggccgtg acgccgcgcg ggctctttct ttccgcggga cgttcctccg gagtgcgtgt
     2461 cccggccttg cggtatggta atctgtcttg aggaaatctc ggaaggagga tcgcgatgcc
     2521 ttacgttgcg aaggatttcg ggacgcttgt cgggatggac ggtttcagcg agacgctgct
     2581 caagaaccac ttcacgctct accaggggta cgtgacgaac acgaacaagc tgtcggacct
     2641 tctcgggggg atgctcgccg aggggaagac ggcaaccccg gagtacgccg aactgaagcg
     2701 ccgcttcggc tgggagtgga acgggatgcg ccttcatgag ttctacttcg aaaacctcgg
     2761 aggcaagggc gccctcgacc cggcctcgaa gctggggaag gcggtcgcct cggagttcgg
     2821 cggcgtcgag aagtgggaag ccgatttccg ggccgcgggc gcgatgcgcg ggatcggctg
     2881 ggtcgtcctc taccaggaca agttgggcgg ccgcctcttc aaccagtggg tcaacgagca
     2941 tgacgtgggg aacccggccg gtgcggtccc gctcttcgtc atggacgtgt tcgaacactc
     3001 cttcatggtc gactacgggc tgaagcgggc cgactacatc gcggcgtttt tccggaacgt
     3061 gaactggaag gcggccgagg ctcggctctc ctgacgacac accggcggct cgcgggagcc
     3121 gcccgcggaa aataccgacg ggctattgcg gggaattccg cgcagtagcc cgtttccatt
     3181 ccggcgttcg gcggaggacc gtggattatt acgcgcaggg gagcctggtc gcggcggtgg
     3241 tggcgctggc catcgcggtg tatctgcgcg tcttcgcgcg gggggacgcg gaggcgaggg
     3301 cgttctccga cctctccctc gtcatcttcc tgtggtgctt ccccgagtac ctgtggaaga
     3361 cgctcccgtc gaccttctgg cacaaggtga gcctggcggg ggtgatgttc atcccaccct
     3421 tcgtccttcg ctactgcggt gccgcggtgc ggagccgccc gcgatggctc ccggcggcct
     3481 tcgtcgccgg cgtctgggcg agcgtcgcgt tcgcggtgac gatcttttac gacccggccc
     3541 tcaactctcc cttgtggaac gtcgccgcgc tcctgttcgt ctacccgtac ctcggcgcgg
     3601 gcctgtacgt catcgtccgc tacgggttcc gcaactcccc ttccgccgtg gagcggaaga
     3661 agtaccagtt cctcgtcgtc gggggcggaa tcgcaagcct tgtggcgccg ctcaactttc
     3721 tccccggcac gggcgtcgac ttcccgccgc tggcggcgtt cgcggtgctc gtcttcttct
     3781 atttcatggc gacggggatc ctgcacctcc acttcttcga gcttcccgac atcgtcgggc
     3841 gcggggtcgt gctggtcatt cagatcttcg cctttgcggt ggtcttcgga atgatggagg
     3901 tcgtctctgg ccggaagttc tggttccccc tcgcgggggt gttcctcatc tcgttcttcc
     3961 tgctctcgtt ctatcctttc ctgatgcgca aactcggcgg catctccgcg gacctgctgg
     4021 tccggcagtc ccgccgggtc cagtcggtgc tgtccgactt cgcgcggaag ctgcccgaat
     4081 cgacggttct cgcggagatc gggagtaccg tggaagaggc cctggcgcag gttccgttcg
     4141 tcgagcgcgc gtcgctgtgg gtccggcccg aggggggaac cgacgagggg atcgggacgc
     4201 ttccggtgcg gcccgacgag tatctgcgcg ccttcacccg cttcacgaag cggtaccccg
     4261 cgttgcggat catggagcac ggcgggtcga aggccgcgga cgtggcgatc tgggacgact
     4321 cgttcgacgc ctcgatcccc cttttcctgc ggggggtcct cgtcgccgtc gtccttttcc
     4381 actggagggg gaagcggccg tcgttccggg agatggacct gctcatcccc ttcctcgacg
     4441 ggatcgcgct ggcggtggag aacatccggc agcagcagcg gatccagcgt cgggagcact
     4501 tcgcgacggt cggggagctg gcggcggggc tggcccacga ggttcgcacg ccggcggggg
     4561 tgatcaaggg ggcggcgcag tttctctccc gcgaggcgcg accgaaggac cgggagttcc
     4621 tcgacatcat cgtcgaggag gcggaccggc tgaacggcgt ggtcacggag ttcctcgagt
     4681 acgcccgccc gacggagcgc aagccgcaga cgatctccct gaaggagccg gtggaaaagg
     4741 cgatcgccct cctcctgcgg gagcgtgggg agcggatggg aacggtgggc cggcacgtgc
     4801 tgacccagga gccggttcca cgggtcaacg cggacccggc cgagatcgag cgggtggtct
     4861 acaatctcct gacgaacgcg atcgaggcga tgccgggaac gggggatctg accgtccgcg
     4921 tgacctcgga aggcggggag gcgcggatcg cggtcgagga caccggcgcg gggatccccg
     4981 aggcggaccg gccccagctc ttccgcccct tcttcacgac gcgtgagcgc
//
LOCUS       DASVJL010000436         4784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_262797, whole genome shotgun sequence.
ACCESSION   DASVJL010000436 DASVJL010000000
VERSION     DASVJL010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262797"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1116
                     /gene="aroF"
                     /locus_tag="VF357_09160"
     CDS             103..1116
                     /gene="aroF"
                     /locus_tag="VF357_09160"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX7524360.1"
                     /translation="MIIVLRAGATDQDVRQIEETIKGKGLTAHISRGVERTIIGAIGD
                     ERKLDPDAFEGLPVVEKVLRILVPYKLVSREFRKEDTVITVNGKTVGGKALALFAGPC
                     SVEGRDMMVGIGERVASAGATFLRGGAFKPRTSPYAFQGLGEEGLKYLAEARDRTGLP
                     VATELMDPRDIDLVARYADVIQIGARNMQNFRLLTEVGKLDKPVILKRGMSATIQEWL
                     MSAEYIASEGNQKIILCERGIRTYETATRNTFDVSSIPVVKSLSHLPVIADPSHAAGK
                     VALVEPLAAAAIAAGADGLMIEVHHQPEKALSDGPQSLRPDAFLQMIGRLRKVAEAVG
                     RTL"
     gene            1121..1975
                     /locus_tag="VF357_09165"
     CDS             1121..1975
                     /locus_tag="VF357_09165"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEX7524361.1"
                     /translation="MARERVGILGLGLIGGSLGLALKGKRGAPEVWGCDRRKETVRRA
                     LSAGAIARGCTEAQLSACDMVIVCVPILRAVEAIRRLGPRMRPGSVLTDAGSVKSGIV
                     REGERAAARGAFFVGGHPIAGTEASGFSAADPDLFYGRSFVVTPTPRTDAGAVLRVER
                     VWKRAGSAVLLRMDPKVHDHVFAYVSHLPHAVAYALVHSVATLPSKVPLGYSAGGFRD
                     FTRIASSNPEMWKDIVLENRTELLRALSHYRRSLALLERRIAAGDADGLLEYFGRAKK
                     TRDGLLST"
     gene            1972..3252
                     /gene="aroA"
                     /locus_tag="VF357_09170"
     CDS             1972..3252
                     /gene="aroA"
                     /locus_tag="VF357_09170"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX7524362.1"
                     /translation="MSGKTVVRGEISVPGDKSISHRAVMFAALATGVSRVRGFLHAAD
                     TLSTAGMMRALGARIDEVSPTELRIEGKGLRDLAEPADVIDAGNSGTTIRIGSGILAA
                     QPFLSVVTGDRYLRRRPMGRVIEPLTRMGAAIQGRDGNRLPPLCIRGGALRGIRYEMP
                     VASAQVKSSLLLAGLYADTPVTVVEPIRSRDHTERMLTAMGAGVTRDGNAVTVAPCGR
                     LDPLEMTVPGDLSSAAFFLVLAAVTPGSSLTVSGVGVNPFRTGVLDVLRRMGADIRHV
                     GERLEGGEPVADLVVRGAELVAARVAPEEVPGLIDEVPVLCVAAAFAAGRTEIRGAGE
                     LRVKESDRIASMVACLSSLGILCGEYPDGLWVEGPAAVRPPSGPCDSRGDHRIAMSMR
                     ILGAAAGVPVEVTDVDCIDTSFPGFQPLLDGLRR"
     gene            3249..3920
                     /gene="cmk"
                     /locus_tag="VF357_09175"
     CDS             3249..3920
                     /gene="cmk"
                     /locus_tag="VF357_09175"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX7524363.1"
                     /translation="MRSRPVVTIDGPSGSGKTTVSKRVAEASGMVRIDTGAMYRAVAL
                     AARSEGVPGGDSARLGGVARELDLSFRTVAGAPRVFLSGEDVSDALRSPEMSMGASAV
                     SAHGPVREALVAKQREMARDGGVVLEGRDTGTVVFPDAEAKFFLDAAAAVRARRRQLE
                     ISPPGAQSFEEVLRDVLRRDVQDSTREHSPLRVADGAVYIDSTTMTIDEVVGEMLRRL
                     PGVPR"
     gene            3917..>4784
                     /gene="ispH"
                     /locus_tag="VF357_09180"
     CDS             3917..>4784
                     /gene="ispH"
                     /locus_tag="VF357_09180"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX7524364.1"
                     /translation="MTKKVLIAKSAGFCFGVKRAIAIADETAGKREAEDGRKGPIQSL
                     GPIIHNPQAVERLQEKGVRVVETVDEITCGKVIIRSHGVTRSDRAALEGKGVAIVDAT
                     CPFVTKAQEHARTLSRNGYAVVVVGDPNHPEVKSIISYIEKGVPVFTSTAEFAAAKGV
                     RKVGIVAQTTQSFENLMAFVAAAMKRFPEVRVFNTICNATALRQQESTGLAGMADVMF
                     VLGGYNSANTRRLAEICKAINPRTHHIETEGELTPSMVEGASVAGVTAGASTPQWIIA
                     GFLERLKELWKCD"
BASE COUNT          744 a         1554 c         1761 g          725 t
ORIGIN      
        1 ccgatacatg gattgtgtgc aatcgggctc gccctttgca ccatataatc atccgctgga
       61 acgggatccg aacgattaca agatagataa ggggacacac gcatgatcat cgtattgcgc
      121 gccggcgcca cggaccagga cgtccggcag atcgaagaga cgatcaaggg gaagggactg
      181 accgcgcaca tctcgcgcgg ggtggagcgc acgatcatcg gggcgatcgg cgacgagcgg
      241 aagctggacc cggacgcgtt cgagggattg ccggtcgtcg agaaggtgct tcggatcctc
      301 gtcccgtaca agctcgtcag ccgggaattc aggaaagagg acaccgtcat cacggtgaac
      361 gggaagacgg tggggggaaa ggccctcgcc ctgttcgccg gtccctgctc cgtcgaaggg
      421 cgcgacatga tggtggggat cggggagcgc gtcgcctccg cgggggcgac gttcctgcgc
      481 ggcggcgcgt tcaagccgcg cacctccccg tacgccttcc agggattggg ggaggagggg
      541 ttgaaatacc tcgcggaggc ccgcgaccgg accgggctcc ccgtggccac cgagctgatg
      601 gacccgcggg acatcgacct cgtggcgcga tacgccgacg tcatccagat cggggcgcgg
      661 aacatgcaga acttccgcct cctcaccgag gtcgggaagc tcgacaagcc ggtgatcctc
      721 aagcgcggaa tgagcgcgac gatccaggag tggctcatgt ccgcggagta catcgcctcc
      781 gaggggaacc agaagatcat cctgtgcgaa cgcgggatcc gaacgtacga gacggccacc
      841 cggaacacct tcgacgtctc ctccattccg gtggtgaagt ccctctccca ccttcccgtg
      901 atcgccgacc ccagccacgc cgccgggaag gtggcgctgg tggagccgct cgccgcggcc
      961 gcgatcgcgg cgggggcgga cggcctgatg atcgaggtcc accaccagcc ggaaaaggcg
     1021 ctctccgacg gaccccagtc gctccgaccg gacgcattcc tgcagatgat cgggcggctg
     1081 cggaaggtag ccgaggcggt ggggaggacg ttgtagcctc gtggcgcggg aacgggtagg
     1141 catcctcggc ctggggctga tcggcggttc cctggggctg gccctcaagg ggaaacgcgg
     1201 cgcccccgag gtgtgggggt gcgaccgaag gaaggagacc gtccgccggg cgctttccgc
     1261 cggcgcgatc gcgcgcggct gcaccgaggc acagctttcc gcgtgtgaca tggtgatcgt
     1321 ctgcgtcccg atccttcgcg ccgtggaggc gatccggcgc ctcggtccgc ggatgcggcc
     1381 ggggtcggtt ctcaccgacg cggggagcgt gaagtccggg atcgtccggg aaggggagcg
     1441 cgctgcggcg aggggcgcct tcttcgtcgg cggccacccg atcgcgggga cggaagcgtc
     1501 gggattttcc gccgccgacc cggacctttt ttatgggcgt tcgttcgtcg tcacccccac
     1561 cccccggacc gacgcgggcg ccgttctccg cgtggagcgg gtctggaagc gcgccggctc
     1621 cgcggttctg ctccggatgg atccgaaggt ccacgaccac gtcttcgcct acgtctccca
     1681 tcttccgcac gcggtggcgt acgccctcgt ccattccgtc gcgaccctgc cctccaaggt
     1741 cccgctcggc tactccgcgg gggggttccg ggacttcacc cggatcgcgt cgagcaaccc
     1801 ggaaatgtgg aaggacatcg tgctcgagaa ccggaccgag ctccttcggg cgctttcgca
     1861 ttatcgccgg agtctcgccc tcctcgaacg ccggatcgcc gcgggcgacg cggacgggct
     1921 cctcgaatat ttcgggcgcg cgaagaagac gcgggacggg ctgctgtcga catgagcggg
     1981 aaaacggtcg tccgcggtga aatttccgtc cccggcgaca agtcgatcag ccaccgcgcc
     2041 gtgatgttcg ccgcgctcgc gacgggggtg agccgcgtcc gcggcttcct ccacgccgcg
     2101 gatacgctgt ccacggcggg aatgatgcgc gccctcggcg cgcggatcga tgaggtctcg
     2161 cctaccgagc tgcggatcga ggggaagggg ctgcgcgacc ttgccgagcc cgccgacgtg
     2221 atcgacgccg ggaactccgg caccacgatc cggatcgggt cggggatcct cgccgcccag
     2281 ccgttcctct cggtggtcac gggcgatcgg taccttcgcc gccgtccgat gggccgcgtg
     2341 atcgagccgc tgacgcggat gggggcggcg atccaggggc gggacgggaa ccgtctcccg
     2401 ccgctgtgca tccgcggcgg agcgctccgg gggatccggt acgaaatgcc ggtggcttcc
     2461 gcgcaggtga agtcgtccct gctgctcgcg ggactgtacg ccgacacccc ggtgaccgtc
     2521 gtggaaccga tccggtcgcg cgaccacacc gagcggatgc tgaccgcgat gggggccggg
     2581 gtcacccggg acgggaacgc ggtcacggtg gcaccctgcg ggcggctcga tccgctggag
     2641 atgacggttc cgggcgacct ctcctcggcc gccttcttcc tcgtcctcgc cgccgtgacc
     2701 ccgggctcgt cgctcaccgt cagcggcgtg ggggtgaacc cgttccgcac gggggtcctc
     2761 gacgtgctcc gccggatggg ggccgacatc cgccacgtcg gggagcggtt ggaggggggg
     2821 gaaccggtgg cggacctcgt cgtccggggc gcggagctcg tggccgcgcg cgtggctccg
     2881 gaagaggtcc ccggcctgat cgacgaagtc ccggtccttt gcgttgcggc ggccttcgcg
     2941 gcgggccgga cggagatccg cggcgcggga gagcttcgcg tcaaggagtc ggaccggatc
     3001 gcgtcgatgg tcgcctgtct ctcttcgctc ggaatccttt gcggggaata cccggacggc
     3061 ctgtgggtgg aggggccggc cgcggtgcgc cccccctccg gtccgtgcga cagccggggg
     3121 gaccaccgga tcgcgatgtc gatgcggatt ctcggcgcgg cggcgggggt tccggtcgag
     3181 gtgacggacg tcgattgcat cgacacgtcc ttcccgggat ttcaaccgtt gcttgacgga
     3241 ttgagacgat gaggagccgt ccggtcgtca ccatcgacgg accctcgggg tcggggaaga
     3301 ccacggtgtc gaaacgggtc gcggaagcct ccgggatggt ccgcatcgac acgggcgcga
     3361 tgtaccgggc ggtcgcgctg gcggcccgaa gcgaaggagt tcccgggggc gactccgctc
     3421 gtctcggagg ggtggcccgg gagctggatc tttcattccg gaccgtcgcg ggggcgccgc
     3481 gcgtcttcct gtcgggagag gacgtaagcg atgctctccg ctcgccggag atgagcatgg
     3541 gggcctccgc ggtctcggcc cacggcccgg tccgcgaagc gctggtggcg aagcagcggg
     3601 agatggcgcg ggacgggggt gtcgtactcg aggggcggga caccggcacg gtcgtcttcc
     3661 ccgacgcgga ggcgaagttc ttcctcgacg ccgcggctgc cgtgcgtgcc cggcggcgcc
     3721 agctcgagat ttcccccccg ggcgcccagt cgttcgagga ggtcctgcgg gacgtcctcc
     3781 ggcgcgacgt gcaggacagc acgcgggagc actcccccct gcgggtggcc gacggcgccg
     3841 tgtacatcga ctcgacgacg atgacgatcg acgaggtggt gggagagatg ctccgacgac
     3901 tgcccggggt cccgcggtga cgaagaaagt cctgatcgcc aagagcgcgg gcttctgctt
     3961 cggcgtgaag cgcgccatcg cgatcgccga cgagacggcc ggcaagcggg aggcggagga
     4021 cgggaggaag ggcccgatcc agtcgctcgg cccgatcatc cacaacccgc aggcggtgga
     4081 gcggctgcag gagaagggcg tgcgcgtcgt ggagacggtc gacgagatca cctgcgggaa
     4141 ggtgatcatc cgctcccacg gggtgacccg ctccgaccgg gcggcgctgg agggaaaagg
     4201 ggtcgcgatc gtcgatgcca cctgcccctt cgtgacgaag gcccaggagc acgccaggac
     4261 attgagccgg aacgggtacg ccgtcgtcgt cgtgggggac ccgaaccacc cggaagtgaa
     4321 aagcatcatc agctacatcg agaagggcgt tcccgtcttc acctcgaccg cggaattcgc
     4381 ggccgcgaaa ggggtgcgca aggtcgggat cgtggcccag acgacgcaat cgttcgaaaa
     4441 cctcatggcg ttcgtcgccg cggcgatgaa gcggttcccc gaagtccggg tgttcaacac
     4501 gatctgcaac gccaccgccc tccggcagca ggagtcgacc ggcctcgcgg gaatggccga
     4561 cgtgatgttc gttctcggcg ggtacaacag cgcgaacacg cgccgcctgg cggagatctg
     4621 caaggcgatc aacccccgga cccaccacat cgagacggaa ggggagttga cgccttcgat
     4681 ggtcgagggg gcgtcggtgg ccggggtgac cgccggggcg tcgacccccc agtggatcat
     4741 cgccgggttc ctcgagcgcc tgaaggagct ttggaagtgc gata
//
LOCUS       DASVJL010000437         5209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_262982, whole genome shotgun sequence.
ACCESSION   DASVJL010000437 DASVJL010000000
VERSION     DASVJL010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262982"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VF357_09185"
     CDS             complement(<1..207)
                     /locus_tag="VF357_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524365.1"
                     /translation="MKTFAAVLAMAFVFAGISPARAEVKTVVVEYRHGDVVLEGYLAY
                     DDSIQGKRPGVLVVHEWNGHNPYVR"
     gene            complement(229..600)
                     /locus_tag="VF357_09190"
     CDS             complement(229..600)
                     /locus_tag="VF357_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524366.1"
                     /translation="MMKIGDVLEPGGRGVIGTASKAGVVSMAVYAIPHIVDKETVAWG
                     MTDGRTWNNVRENPNASYAYFAPGEGFRGTRLTLSLSRTEDSGEMLAKIREQTGASSP
                     GNPEAVKHVAYFKVVETRPLV"
     gene            complement(597..1817)
                     /gene="mutY"
                     /locus_tag="VF357_09195"
     CDS             complement(597..1817)
                     /gene="mutY"
                     /locus_tag="VF357_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX7524367.1"
                     /translation="MTDGRQETVRGVARSSEPGELRRVHSRTRRIATERPAFLRGSRP
                     FFFKEDPRTAAWISAALLGWFRTAARSMAWRETRDPYRIWISEIMLQQTRVETVAPYY
                     ERFLLKFPDVGSLARAPLDTVLKAWEGLGYYSRARNLHRAAGILVARHGAKVPRTVEA
                     LEALPGIGRSTAGAIAAIAFEADVPILDANARRVVARLFAVEGDPRSSAVENVLWERS
                     ARLVRKGKGRETALAVMDLGAVVCLPKAPRCRECPLRSRCSSCLRGLQDSIPPKRAKK
                     TLLHHDVVAAVFRRADGALFLMRRPSDGLLGGLWAFPSGRRDPGETLEDALRRSLWEK
                     LRVRAVIQREVGAVRHAYSHYRITLHGFLCENAGGALPAGEGTGWLPGHGDGKFALPR
                     ADRKLVDFIHREEE"
     gene            1911..2834
                     /gene="trxB"
                     /locus_tag="VF357_09200"
     CDS             1911..2834
                     /gene="trxB"
                     /locus_tag="VF357_09200"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX7524368.1"
                     /translation="MIRDVVILGSGCAGSTAAIYAARANLVPLVLDGREPGGQLTLTT
                     EVENFPGFPAGIQGPELVDFMRKQAERFGAAYLAETVIGVDLSKRPFAVNTEENTYLA
                     RTLIVATGASARLLGLESEKKLMGRGVSTCATCDGAFFREREIVVVGGGDTALEEAIF
                     LTRFASKVLLVHRRDQFRASKIMVDRARNLPKIEFVLDTIITDILDPAKNEVSAVRLK
                     STKDGTEEVRKVDGVFVAIGHDPNTKIFEGQLNLDNGYVVLGDGAKTSIEGVFAAGDV
                     HDKVYRQAITAAGAGCRAAIEAERFLEAEGR"
     gene            2843..3346
                     /locus_tag="VF357_09205"
     CDS             2843..3346
                     /locus_tag="VF357_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7524369.1"
                     /translation="MRIYLVRHSEAVDRVPPMPDAARYLSARGRVSFREMARRFRDAG
                     GLPSRIFTSPFLRAVQTAEILSETLRYDGEVVVDSRLAPGFDVSKLIAVLDDCPSERE
                     IAFVGHEPDMGGLLTRLLSLPQQYAMRKGAIAALDLPIAGDPLRVDLAWLLTGDRRID
                     DPATLMT"
     gene            3541..4911
                     /locus_tag="VF357_09210"
     CDS             3541..4911
                     /locus_tag="VF357_09210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7524370.1"
                     /translation="MPFQQFGLSPEILRAVQEMHYTTPTPIQEKAIPDVLQGKDLLGS
                     AQTGTGKTAAFALPILHRLAAETRTVRGRRLVRSLVLTPTRELAMQIGESFGDYGRHT
                     GMRHAVIYGGVSQKTQEQALQKGVDILVATPGRLLDLMGQKLINLSTVEIFVLDEADR
                     MLDMGFIVDIRRIIEKLPAKRQTLMFSATMPSEIVRLSATLLRDPVRVQVAADAAPAD
                     DVDHRLYYVEKSSKPDLLKTLLAGDEIKNALVFTRTRHGADRVERTLSRAGVRVEAIH
                     GDKSQGARERALSAFKKGTIRVLVATDIAARGLDIVDLSHVVNYDLPNEPEAYVHRIG
                     RTGRAGASGTAISFCGFEERPLLAEIERLMSKHLTVVTDHPFASPARPAPPTVFHTGR
                     TPSKPRPFMISVDAAFLSAGRTASSSPPERVSRHAGNEARPTSGPGGRKSGHRDQTSS
                     RGRSQR"
     gene            complement(4967..>5209)
                     /locus_tag="VF357_09215"
     CDS             complement(4967..>5209)
                     /locus_tag="VF357_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524371.1"
                     /translation="FSGDRCGDHLDHLQQDESGKSPCAEGVDPGDQPLPVHEKMEEAE
                     MVQGETESEEREEKDERLDEEFQLFLQRTVPRLALR"
BASE COUNT          918 a         1805 c         1589 g          897 t
ORIGIN      
        1 ccggacatac gggttgtggc cgttccattc gtgaaccacg aggaccccgg gccgcttccc
       61 ctggatcgag tcgtcgtacg ccaggtaccc ttccagcacg acgtctccgt gccgatattc
      121 caccactacg gttttcacct ccgcccgcgc cggggaaatc ccggcgaaca cgaaggccat
      181 cgcgagaacc gccgcaaatg ttttcatctc cttgcctcct cctcttcgtc atacaagggg
      241 tcgggtttcg accaccttga agtacgccac gtgcttgacc gcctccgggt tcccggggct
      301 cgaggcaccg gtctgctcgc ggatcttcgc tagcatctct cccgaatcct ccgtgcggga
      361 aagggagagt gtcagccgtg tcccgcggaa cccctctccc ggggcgaaat acgcatagga
      421 cgcgttcggg ttctcccgta cgttgttcca cgtcctgccg tccgtcatcc cccacgcgac
      481 cgtttccttg tccacgatat gtggaatcgc gtacaccgcc atgctgacca ccccggcctt
      541 cgacgcggtg ccgatcacgc cgcgacctcc cggctccaga acgtccccga ttttcatcat
      601 tcttcctccc gatggatgaa atcgaccagc tttcgatcgg cccgtggaag ggcgaacttc
      661 ccgtccccgt gccccgggag ccaccccgtc ccctctcccg ccggcaacgc accccccgca
      721 ttctcgcaaa gaaatccgtg gagggtgatc cggtagtggg aataggcatg tcgtacggct
      781 cccacctccc tttgaatcac cgcgcgcacc cgaagtttct cccaaagaga acggcggagc
      841 gcatcctcaa gggtctcccc cgggtcgcgc cttcccgaag ggaaggccca caatcccccc
      901 agaagcccat cggacgggcg tcgcatcagg aacagcgcgc cgtccgcgcg gcggaacacc
      961 gcggccacga catcgtgatg aaggagggtt ttcttcgccc gcttcggagg aatcgaatcc
     1021 tgaagccccc ggaggcagga ggagcagcgg gatctcaggg gacactcgcg gcaacgaggg
     1081 gccttcggca ggcagacgac ggcgcccagg tccattaccg cgagagctgt ctcccgaccc
     1141 tttcccttcc gcaccagacg cgcggaccgt tcccaaagga cgttctccac cgcggaggaa
     1201 cggggatccc cttcgacggc gaaaagacgg gcgacgacgc gacgggcgtt cgcgtcgagg
     1261 atcgggacgt ccgcctcgaa ggcgatcgcg gcgatcgctc cggccgtcga tcggccgatc
     1321 cccgggagag cctcgagcgc ctccaccgtg cgcggcacct tggcgccgtg acgggcaacg
     1381 aggatcccgg cggcccgatg gaggttcctc gcgcgggaat agtacccgag tccctcccac
     1441 gccttcagga cggtgtccaa cggcgcacgg gcaagggatc ccacatctgg aaatttcagt
     1501 agaaaacgtt cgtagtaggg agcgacggtt tcgacccggg tctgctgaag catgatttcc
     1561 gagatccaga tgcgatacgg atcccgggtc tcccgccacg ccatcgaacg ggccgccgtc
     1621 cgaaaccacc ccaggagcgc ggcggagatc cacgcggcgg ttcgcggatc ttccttgaaa
     1681 aagaacggcc ggctcccgcg gaggaatgcc ggccgttccg tcgcgattcg ccgcgttcgg
     1741 gaatgtactc tcctcaattc gcctggttca gaagatcgag cgactccgcg gacggtttct
     1801 tgccggccat cggtcatccc tccttcgttc tttgtggaaa tttcttcttg ccggggtatt
     1861 cttacacgga ggggtgcgga aagtcacccc ccgacggagg aacccaccgc atgatccgcg
     1921 acgtcgtcat cctcggttcc ggctgcgcgg gctcgaccgc cgccatctac gccgcgcgcg
     1981 ccaacctcgt cccgctggtg ctggatggcc gcgaaccggg gggacagctg acgctgacca
     2041 ccgaggtcga gaactttccc gggttccccg cggggatcca gggcccggag ctcgtcgact
     2101 tcatgcggaa gcaggcggaa cggttcggag ccgcgtacct tgccgaaacg gtcatcggcg
     2161 tggacctttc gaaacgcccc ttcgcggtga acacggagga gaacacctac ttggcgcgca
     2221 ccctcatcgt cgccaccggc gcctcggcca ggctcctggg cctggaatcg gagaagaagc
     2281 tgatggggcg gggtgtgtcc acctgcgcca cctgcgacgg ggcgttcttc cgggaacgcg
     2341 aaatcgtcgt ggtcggcggg ggcgacaccg cgctggagga ggcgatcttc ctcacccggt
     2401 tcgcctcgaa ggtgctgctg gtccatcgaa gggaccagtt ccgcgcctcg aaaatcatgg
     2461 tcgatcgggc ccgcaacctc ccgaagatcg agttcgtcct cgacaccatc atcacggaca
     2521 tcctcgaccc ggcgaagaac gaggtctccg cggtccggct gaagagcaca aaggacggta
     2581 cggaggaggt ccgaaaggtc gacggtgtct tcgtcgccat cggccacgat ccgaacacga
     2641 agatcttcga ggggcaattg aatctcgaca acgggtacgt cgttctgggc gatggcgcga
     2701 aaacaagcat cgagggggtc ttcgccgccg gagacgtgca cgacaaggta taccggcagg
     2761 cgatcacagc ggcgggggcc ggctgccgtg ccgccatcga ggcggagcgg ttcctcgagg
     2821 cggaaggccg gtaagaccga cgatgcggat ctatctcgtt cgccattcgg aggcggtgga
     2881 ccgtgttccc ccgatgccgg acgcggcgcg atacctgagc gcccggggac gcgtttcgtt
     2941 ccgggaaatg gctcgccgtt tccgtgatgc cgggggactt ccctcccgga ttttcaccag
     3001 cccgttcctc cgggcggtgc agaccgcgga gatcctgtcg gagacgctcc ggtacgacgg
     3061 ggaggtcgtc gtcgattcgc ggcttgcccc cgggttcgac gtttcgaaac tgatcgccgt
     3121 gctcgacgat tgtccgtccg aaagggagat cgcgttcgtc ggacacgagc cggacatggg
     3181 cggtcttctc acccgcctgc tctccctccc ccaacagtac gcgatgcgaa agggcgccat
     3241 cgccgccctc gatctcccaa tcgccgggga cccgctccgt gtcgatctcg cctggctcct
     3301 taccggggat cgccggatcg atgacccggc gaccctgatg acgtaggacg ggggatttgc
     3361 cgggacatca cgacgccggg tgttgcaaca cccgtcacgg tgtgataagt tgtactcgat
     3421 ttgagccctc tcgatctcaa aacccccgcg gcgttccagg tacccccggc gattatcgac
     3481 ccgtagaccc tgcgtccgtc aaccatctct catccgacca tcaggagaaa aaggaaaacg
     3541 atgccgtttc aacaattcgg tttatccccg gagatcctcc gggccgttca ggaaatgcac
     3601 tacacgaccc cgacccccat ccaggaaaag gcgatccccg acgtgctcca ggggaaggat
     3661 ctgctcggct ccgcccagac cggcaccgga aagacggccg ccttcgcgct gcccatcctt
     3721 catcgcctcg ccgcggagac caggaccgtc cgggggcgcc ggctcgtccg atcgctcgtc
     3781 ctcaccccga cgcgggaact tgccatgcag atcggggaaa gtttcggcga ctacgggcga
     3841 cataccggga tgcggcacgc cgtcatctac ggcggcgtca gccagaaaac tcaggagcag
     3901 gcgctgcaga aaggggtgga tatcctcgtc gccacgccgg gccgtctcct cgacctcatg
     3961 gggcagaagc tcataaacct ctccaccgtc gaaatcttcg tcctggacga ggcggaccgg
     4021 atgctcgaca tgggcttcat cgtcgacatc cgccgcatca tcgagaaact ccccgcgaaa
     4081 cggcagacgc tgatgttctc cgcgacgatg ccgtcggaga tcgtccgcct ttccgccaca
     4141 ctgctccgcg atccggtgcg ggtccaggtc gccgccgatg ccgcgccggc cgacgacgtg
     4201 gatcatcgtc tctattacgt ggaaaaatcg tccaagccgg atctgttgaa aacacttctc
     4261 gccggcgatg agatcaagaa cgccctcgtc ttcacgcgga cgcggcacgg cgccgatcgc
     4321 gtggagcgga ccctgagccg cgcgggagtg cgcgtcgaag cgatccacgg ggacaagtcg
     4381 cagggggcga gggaacgcgc cctttccgcc ttcaagaagg ggacgatccg ggtcctggtg
     4441 gccacggata tcgccgcccg gggcctcgac atcgtcgacc tctcccacgt ggtcaactac
     4501 gacctcccga acgagccgga ggcgtacgtc caccggatcg gccgcaccgg gcgggccggc
     4561 gcgtcgggta ccgcgatctc cttctgcggc ttcgaggaac gcccgctgct cgccgagatc
     4621 gagcgcctga tgagcaagca cctgaccgtg gtcacggacc accccttcgc ctcaccggcg
     4681 aggccggcgc ccccgacggt tttccacacg ggccggacgc cgtcgaaacc acggccattc
     4741 atgatctccg tggacgccgc gttcctctcc gcggggcgaa ccgcctcctc ttcccctccg
     4801 gagcgggttt cccgtcacgc cgggaacgag gcgcgcccga cctccggccc cggcggccgg
     4861 aagagcgggc accgggacca gacttcttcc cggggccgct cccagaggta accgcagaac
     4921 cgcaattccc gatcgggccg cccccgcgga cgcgtttcgt gtatcatcat cgcaacgcca
     4981 accgggggac ggtccgctgc aggaaaagct gaaattcctc gtcaagccgc tcgtctttct
     5041 cttcgcgttc ctcgctctca gtttctccct gtaccatctc ggcctcttcc attttttcat
     5101 gaaccgggag cggctgatcg cctggatcca ctccctcggc gcatggggat ttgccggatt
     5161 catcctgctg caggtgatcc aggtggtcgc cgcacctatc cccggagaa
//
LOCUS       DASVJL010000438         5313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1669290, whole genome shotgun sequence.
ACCESSION   DASVJL010000438 DASVJL010000000
VERSION     DASVJL010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669290"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VF357_09220"
     CDS             <1..461
                     /locus_tag="VF357_09220"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7524372.1"
                     /translation="ARLMFVGEGPGSEEDRLGEPFVGAAGKRLDRWIARIGLHREDVY
                     IANIVKCRPPGNRAPLPDEAKACLPYLLRQIRAIRPEVVCTLGATALNFLLGVEEKIT
                     RERGKWRERDGVPVLPTYHPAFILRDASRETEVFGDFDAIASRLRLPSAK"
     gene            458..1651
                     /locus_tag="VF357_09225"
     CDS             458..1651
                     /locus_tag="VF357_09225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX7524373.1"
                     /translation="MTDFLPEVRVSRKGEERLHTGHAWVFGDDLREVPPGLSPGQWTW
                     VRSRSGEPLGTATINLGTRIALRRVSRADVLPTEAFLGDRLCEASARRAEAGMGGERA
                     LRVLYSEGDFLPGMIADRYGDVLSVQILTAGMEIVRDLLLDVLERHFRPRLIYERSEG
                     GGRRHEGLPERKGPARGEGPTREEIETDGVRFSVDVESGPKTGFFLDQRRNRGIVRDL
                     ARGKAVLDGFCAAGGFGMYALAGGAKSVLAIDSSRAAVETARANASLNGVSGQWEGEA
                     EDLFQALRNLRDVGRRFDLVILDPPSFAKSREGREGALRGYRDINRMGLSVLSPGGVL
                     ATSSCTQLIDGTQWVEALRDAAADAGADLERIAWGGQPPDHPVLLSVPETDYLKFAVY
                     RKRLP"
     gene            2116..3411
                     /locus_tag="VF357_09230"
     CDS             2116..3411
                     /locus_tag="VF357_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX7524374.1"
                     /translation="MKNLFIAVLILLSIPGAALPAGPPGKGVLVATIAAPIGPVTADY
                     LSAVIARAEEEDAALLVVELDTPGGLDSAMREMVQAILKSRVPVAVYVSPQGARAASA
                     GVMITLAADVAAMAPGTNIGAAHPVNMGGGAMDNTMSRKVENDAAAYARSLAAGRGRN
                     VEWAESAVRESASLSEKEALDKKVVDLVAASLPDLLARIDGREVRKGDGTVTLRTKGV
                     PVTRVPMGLRHRVLSALANPNIAYILLMIGIYGIYFELASPGAVFPGVVGGISLLLGF
                     YALQTLSANYAGFLLILLSLLLFFLELKIQSHGALAIGGIVAMILGSLMLFQGSADPY
                     LRVSWAVLGTMVALSTGFFAAVISLAVRSQLRKPSTGSEGMIGEIGEALTDIDRKGKV
                     RVVGEQWDARCDRPVRKGDPVIVKGRDGMTLIVEPKEDR"
     gene            3458..4222
                     /locus_tag="VF357_09235"
     CDS             3458..4222
                     /locus_tag="VF357_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX7524375.1"
                     /translation="MYAAAVVLVLLFLSSAVKILNEYERGVVFRLGRVIGSKGPGLIL
                     LIPVIDKMIRVDLRVVAMDVPAQDVITRDNVTIKVSAVLYFRVIDPNRAIVGVENYLY
                     ATSQLSQTTLRSVCGQAELDDLLASREKINTHLQEILDKDTEPWGVKVAKVEIKNIDL
                     PQEMQRAIAKQAEAERERRAKVIGAEGEFQAAQKLSDAAKIISENPIALQLRYLQTLR
                     EVAAENNSTTIFPVPIDLLTPFMQMAKMVTPAPPKD"
     gene            4222..>5313
                     /locus_tag="VF357_09240"
     CDS             4222..>5313
                     /locus_tag="VF357_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX7524376.1"
                     /translation="MKPLLLFAAGLLVLSGCAGAPPREPARREAPPASRPSIGPAPTR
                     PGITTPPGAVVPSPFAATGTRFIRVLLSGGTSGVVLEGETIRAWGVDGSLAAEAAGRV
                     TLTAVGERIRWNGSRLFGDTLDVAGGPNLHVGKRKVSGRVRLLARKGELLAVAVVPLE
                     EYVAAVVSREAAPRFHPEALAAMAVAARTYAAGAAAKPRDPAYDVVGGVEDQVFDGMD
                     GVTAVFREAADRTRGVVVRYRGELALTVYHSTCGGRTEDAGSAWGKDIPYLRSQPCDD
                     CAESPVYRWEYRMSEAEERRVAKALGVPSGKDLRIAVTGRTPTGRASRVLISSDGVSR
                     ELNAAVFRKAAGYARVRSLKMEIVPAAGEW"
BASE COUNT          812 a         1729 c         1915 g          857 t
ORIGIN      
        1 gcgcccggct gatgttcgtc ggcgagggac ccgggtccga ggaggaccgc ctgggggagc
       61 cgttcgtcgg ggccgccggg aagcggctcg atcggtggat cgcgcggatc ggccttcatc
      121 gggaggacgt ctacatcgcc aacatcgtga agtgccgccc gccgggaaac cgcgcccccc
      181 tccccgacga ggcgaaggca tgccttccgt acctgttgcg ccagattcgc gctatccgcc
      241 ccgaagtcgt ctgcaccctc ggcgcgaccg cgctgaattt cctgttggga gtcgaggaga
      301 agatcacccg ggagcggggg aagtggaggg aacgggacgg cgtccccgtg ctccccacgt
      361 atcacccggc cttcatcctg cgcgacgcct cccgggagac ggaagtgttc ggagacttcg
      421 atgccatcgc ttcgcggctc cgccttcctt cggcgaaatg acggattttc ttcccgaggt
      481 ccgggtcagc cggaaagggg aggaacggct gcacaccggc cacgcgtggg tgttcgggga
      541 cgacctgcgc gaggtgccgc cgggcctttc gccggggcag tggacctggg tgcgctcgcg
      601 gtcgggggaa cctctcggga cggcgacgat caacctcggg acccggatcg cccttcgacg
      661 ggtgtcccgg gccgatgtcc tgccgacgga ggcgttcctc ggggaccggc tgtgcgaggc
      721 gtccgcgcgg cgcgccgagg cgggaatggg cggggaacgc gccctgcggg tcctctactc
      781 cgagggggat ttcctcccgg ggatgatcgc ggaccggtac ggcgatgtcc tctcggtgca
      841 gatactgacg gcggggatgg agatcgtccg ggatcttctc ctcgacgtcc tcgagcgtca
      901 cttccggccg cgcctgatct atgagcgatc cgaaggcggg ggacgtcgcc acgaaggcct
      961 gccggagcgg aaggggccgg cacgcgggga ggggccgacc cgggaggaga tcgagacgga
     1021 cggggtccgt ttttccgtgg acgtggagtc gggaccgaag acgggcttct tcctggacca
     1081 gcgaaggaat cgcggcatcg tccgcgacct ggcgagaggg aaggcggtac tcgacggatt
     1141 ctgcgccgcg ggagggttcg gcatgtacgc actcgccgga ggtgcgaaga gcgtcctggc
     1201 gatcgactct tcgcgtgcgg ccgtcgagac cgcgcgggcg aacgcctcgt tgaacggcgt
     1261 atccgggcag tgggagggag aggcggagga cctcttccag gcgcttcgga atttgaggga
     1321 cgttgggcgg cggttcgacc tggtgatcct cgacccgccg tcgttcgcca agtcccggga
     1381 ggggagggaa ggggcgctgc gcggatacag ggacatcaac cggatggggc tttcggtcct
     1441 ctctcccggg ggcgttctcg ccacgtcgtc ctgcacgcag ttgatcgacg ggacgcaatg
     1501 ggtcgaggcc ctccgggacg cggcggccga cgccggcgcc gacctcgaga ggatcgcgtg
     1561 gggagggcag ccgcccgatc acccggtgct cctctccgtt ccagagaccg actacctgaa
     1621 attcgccgtc tacaggaagc gactgccatg aagcattccc gcagcaaatc gatatcctcg
     1681 ttcattgacg cgttgctttc gggctcgtgc cccccgtttt tcctgctgcg ggggtacccc
     1741 ggcggcgcgg agctcgtacc ggggcggtgg gcttcctctc tgtcccgccg accctgcctt
     1801 cccttccgtg gaatcaccct ccctcgctac gctcaccgga cgtccctgtc cggctcgctg
     1861 gcacctcccc gacctcccgc cctcctggct ccggccgggt cagatgcccg ctcgggaggg
     1921 gatcccacgg aaggtacggc gatcgggcga atattccccc cggaatcccc gatgcccatg
     1981 gatgggcaag tgccgcggga gccagggatg gcgggagcgg ccgctgtgcc ccccgttttt
     2041 cctactgcgg gggtacccca gcagcgcgga gctcgtaccg gggcaccctt cgcaacgggt
     2101 atccttctat gtggaatgaa gaacttgttc atcgcggtcc tgatcctcct ctccattccg
     2161 ggggccgccc ttcccgcagg tccaccgggg aagggggtgc ttgtggccac catcgccgcc
     2221 ccgatcggcc ccgtcacggc ggactacctc tccgcggtca tcgcccgtgc ggaggaagag
     2281 gacgcggcgc tcctcgtggt cgagctggac acgcctggcg ggctcgattc cgcgatgcgg
     2341 gagatggtgc aggcgattct caagagccgg gtccccgtcg cggtttacgt ctccccgcag
     2401 ggggcgcggg cggcgtccgc cggggtgatg atcacgctgg cggccgacgt ggccgccatg
     2461 gcccccggga ccaacatcgg cgcggcccac ccggttaaca tggggggcgg cgcgatggac
     2521 aacacgatgt cgcggaaggt ggaaaacgac gccgcggcct acgctcgctc gctggccgcg
     2581 ggccgggggc gaaacgtcga atgggcggag agcgccgtcc gggagagcgc ctccctctcg
     2641 gaaaaagaag ccctcgacaa gaaggtcgtc gacctggtcg ccgcgtccct tcccgacctg
     2701 ctcgcccgga tcgacgggag ggaggtccgg aagggcgatg ggacggtgac cctccggacg
     2761 aagggtgtgc cggtcacccg cgtgcccatg ggccttcgcc atcgcgtcct gtccgcactg
     2821 gcgaatccca acatcgccta catcctgttg atgatcggga tctacgggat ttacttcgag
     2881 ctggcctccc ccggcgcggt gttccccgga gtggtgggag ggatctcgct gctgctgggt
     2941 ttctacgctt tgcagaccct ctcggcgaat tacgccggct ttctgctgat cctcctctcc
     3001 ctcctcctct tcttcctcga gctgaagatc cagtcgcacg gggcgttggc catcggcggg
     3061 atcgtcgcga tgatcctggg gagcctgatg ctcttccagg ggagcgccga cccgtacctg
     3121 cgcgtctcct gggccgtgct gggcaccatg gtcgcgctgt cgacgggctt cttcgccgcg
     3181 gtcatctcgc tcgccgtgcg tagccagctc cgcaagccgt ccaccggttc ggaggggatg
     3241 atcggagaga tcggggaggc actcacggac atcgaccgga aggggaaagt gcgcgtcgtc
     3301 ggagagcagt gggacgcgcg gtgcgaccgg cccgtccgca agggggaccc ggtgatcgtg
     3361 aaaggccgcg acggaatgac gctcatcgtc gaaccaaagg aagaccgctg accccgggaa
     3421 cggaacgagg cggcacaagg aggattgaaa aatggttatg tacgcagcgg ctgtggtgct
     3481 cgttttgctt ttcctgtcca gcgccgtcaa gatcctgaac gagtacgaac ggggggtcgt
     3541 cttccggctc gggcgcgtga tcgggtcgaa ggggcccggt ctcatcctgc tcatccccgt
     3601 catcgacaag atgatccggg tggatctgcg cgtcgtggcg atggacgtcc ccgcgcagga
     3661 cgtcatcacg cgggacaacg tcaccatcaa ggtgagcgcc gtcctttact tccgcgtcat
     3721 cgacccgaac cgggcgatcg tcggagtgga gaactacctc tacgcgacgt cccagctctc
     3781 ccagacgacc ttgaggtcgg tctgcggcca ggccgagctc gacgatctcc tcgcgtcccg
     3841 ggagaagatc aatacgcacc tccaggagat cctcgacaag gacacggagc cgtggggcgt
     3901 caaggtcgcc aaggtggaga tcaagaacat cgacctgccc caggagatgc agagggcaat
     3961 cgccaagcag gccgaggccg agcgggagcg gcgggccaag gtcatcggcg ccgaggggga
     4021 gttccaggcg gcccagaaac tttccgacgc ggcgaagatc atcagcgaaa acccgatcgc
     4081 cctgcagctg cgctacctcc agacgctgcg ggaagttgcg gccgagaaca actcgacgac
     4141 catcttcccc gtgccgatcg acctgctcac cccgttcatg caaatggcga agatggtgac
     4201 cccggcgccc ccgaaggact gatgaagccc cttcttcttt tcgcggcggg gctcctcgtc
     4261 ctctcgggct gcgcgggggc gcctccgcga gagcctgcgc gccgggaggc tcctcccgcg
     4321 tcgcggcctt ccatcggccc ggcgccgacg cggccgggga tcaccacccc tcccggtgcc
     4381 gtcgtcccat cccccttcgc ggcgacgggg acccggttca ttcgtgtgct cctctcggga
     4441 gggacgtcgg gtgtcgtcct cgaaggggag acgatccggg cgtggggcgt cgatgggagc
     4501 ctggccgccg aggcggcggg gcgcgtcacc ctgaccgcgg tcggggaacg gatccgatgg
     4561 aacgggtcga ggctcttcgg cgacaccctc gacgtcgcgg gggggccgaa cctgcacgtc
     4621 gggaagcgga aagtgtccgg ccgggtccga ctcctcgcgc ggaaagggga actgctcgcc
     4681 gtcgcggtgg tgccgttgga ggaatacgtc gctgccgtgg tcagccggga ggccgcgccg
     4741 cggtttcacc cggaggcgct cgcggcgatg gcggtggccg cgaggacgta cgccgccggg
     4801 gcggccgcga agccgcgcga ccccgcgtat gacgtcgtcg gcggcgtgga agaccaggtg
     4861 ttcgacggaa tggacggggt caccgcggtc ttccgggagg cggcggaccg gacgcggggg
     4921 gtggtggtgc ggtaccgggg cgagctcgcg ctgacggtgt accactccac gtgcgggggg
     4981 cggaccgaag acgccggatc cgcctggggg aaggacatcc cgtacttgcg gtcgcagccc
     5041 tgcgacgatt gcgccgaaag cccggtctac cggtgggaat accggatgtc cgaggcggag
     5101 gagcgccgcg tggcgaaagc gctcggtgtc ccgtccggaa aagatcttcg gatcgccgtg
     5161 acgggccgca cgccgacggg acgggcgagc cgtgtcctga tctcctccga cggcgtctcc
     5221 cgggagctga acgccgcggt gttccggaag gcggcagggt acgccagagt ccgaagtttg
     5281 aagatggaga tcgtccccgc cgccggggag tgg
//
LOCUS       DASVJL010000439         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_451035, whole genome shotgun sequence.
ACCESSION   DASVJL010000439 DASVJL010000000
VERSION     DASVJL010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451035"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VF357_09245"
     CDS             complement(<1..1087)
                     /locus_tag="VF357_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7524377.1"
                     /translation="MVNFDPTPEQEALREMAHKFAANEIRPRAVEWDRDSVFPLELFR
                     KAFDLGLMTGFIPEIYGGQGLTMLDTCILEEEISWGCSGVATSMNANALALGPILLSG
                     TGEQKRVFVQPFATEFRFASFCMTEPGAGSDAGGIATTARRDGDGYVLNGRKCFITNG
                     AHASQYTVFASTDRSRGHRGLSAFIVPRETPGVSSGKKEDKMGQRASETSDVLFEDVR
                     VPEPNRLGAEGEGFKIAMRTLDYARAGVAAMAVGVARAAYEHAAEYGRQRVQFGQPIA
                     MNQAIYFLLADMSIDIEAARLLTWKAAWLADQGKRNTKEASFAKAFAADLAMRAATDA
                     VQIFGGYGYMKDYPVEKLMRDAKLLQIF"
     gene            complement(1092..1715)
                     /locus_tag="VF357_09250"
     CDS             complement(1092..1715)
                     /locus_tag="VF357_09250"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7524378.1"
                     /translation="MARKGPNGGGEKRDRILRAAVKIFSRKGFFNSKVSEIARDAEVA
                     DGTIYLYFRNKDDLLISLFEEKMGEVVADVRRRIAVGGNALEKLRIFIENHMDLLERE
                     SGLVEVLLVELRQSTKFLKDYTPVKFFEYLEIISDILEEGKKEGILRPDLNISIARRA
                     IFGALDELSLTYILSRKPKYHPTVTAAELCRILLEGLCVPGASAGKG"
BASE COUNT          325 a          663 c          562 g          351 t
ORIGIN      
        1 cgaagatctg cagcagtttc gcgtcccgca tcagtttctc cacggggtaa tccttcatgt
       61 agccgtaccc gccgaagatc tggaccgcgt cggtggcggc gcgcatcgcg aggtcggccg
      121 cgaacgcctt ggcgaaggac gcctccttcg tgttccgctt cccctggtcc gcgagccacg
      181 ccgccttcca cgtcagcagc cgcgccgcct cgatgtcgat cgacatgtcc gcgagtagga
      241 aatagatcgc ctggttcatc gcgatcggct gcccgaactg gacccgctgg cgcccgtact
      301 ccgccgcgtg ctcatacgcc gcccgggcaa cgcccaccgc catcgcggcc actcccgcgc
      361 gcgcgtagtc gagcgtccgc atcgcgatct tgaatccctc cccctcggcc ccgagccggt
      421 tgggttcggg aacgcggaca tcctcgaaga ggacatcgga agtctccgaa gcgcgctgcc
      481 ccattttgtc ttccttcttc ccggaggaga ccccgggcgt ctcccgcgga acgatgaagg
      541 ccgacaagcc cctgtgccct cgggaccggt cggtcgacgc gaacacggtg tactgggaag
      601 cgtgtgcgcc gttcgtaatg aagcatttcc ggccgttcag gacgtacccg tcgccgtccc
      661 ggcgagccgt cgtggcgatt ccgccggcgt ccgagcccgc ccccggttcg gtcatgcaga
      721 aggaggcgaa ccggaactcc gtcgcgaacg gctggacgaa cacccgcttc tgctccccgg
      781 tgccagacag gaggatcggc cccaaggcga gggcgttggc gttcatcgag gtggcgacgc
      841 ccgaacatcc ccaggagatc tcctcctcga gaatgcacgt atcgagcatc gtcagcccct
      901 gtcccccgta gatttcgggg atgaacccgg tcatcagccc gaggtcgaac gccttccgga
      961 acagttccag agggaagaca ctgtcccggt cccactccac cgcccgcggc cggatctcgt
     1021 tggccgcgaa cttgtgggcc atctcccgga gcgcctcctg ctccggcgtc ggatcgaaat
     1081 tcaccatgcc gtcagccctt cccggcggac gccccgggca cgcacaaccc ctccagcagg
     1141 atccggcaca gctcggcggc ggtcacggtc ggatgatact tcggtttcct cgagaggata
     1201 taggtgaggg acagctcgtc cagcgcgccg aagatggcgc gacgcgcgat gctgatgttc
     1261 aggtcggggc ggaggatccc ctccttcttc ccctcctcga ggatgtcgct gatgatctca
     1321 aggtactcga agaacttcac cggggtgtag tctttcagga actttgtgct ctgccgcagc
     1381 tccaccagga ggacctcgac gagtccggac tcgcgctcca ggagatccat gtgattctct
     1441 atgaagatcc tgagtttttc gagggcgttc ccgccgacgg cgatccgccg gcgaacgtcc
     1501 gccaccacct cccccatctt ctcttcgaaa agggagatca acaggtcgtc cttgttcctg
     1561 aagtagaggt agatggtccc gtccgcgact tccgcgtccc gggcgatctc ggacaccttc
     1621 gagttgaaga accctttccg ggagaagatc tttacggcag cgcgaaggat ccggtcccgt
     1681 ttctctccac cgccgttcgg tcccttcctc gccattgcaa cctcccctca tgaatgaata
     1741 cccattcact caggaaggta ccttccctca acgggcatgt caaggcgggg ctattcccgg
     1801 aaaccgaggc gcgccgagag ggcctttccc gcccgatcga tcgccgggcc gatcacggtg
     1861 gcgatccttt catcggaaag ccggtgcgcc ggaccggaaa c
//
LOCUS       DASVJL010000440         1870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_75364, whole genome shotgun sequence.
ACCESSION   DASVJL010000440 DASVJL010000000
VERSION     DASVJL010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75364"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VF357_09255"
     CDS             <1..419
                     /locus_tag="VF357_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7524379.1"
                     /translation="VREAMRTEVWSVIPDDSVEDALLILTREKFGALPVLSGDRLVGI
                     ITKADLLNAFVELLDVNGVCFCVDVTFPRNMVRFEELIDTFAGMGVEVRSVLIAPQGE
                     YGALNAHLRIATIDGPAVRRVLSEKGFMIAEQVSRL"
     gene            complement(423..>1870)
                     /gene="acnA"
                     /locus_tag="VF357_09260"
     CDS             complement(423..>1870)
                     /gene="acnA"
                     /locus_tag="VF357_09260"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX7524380.1"
                     /translation="RLDQGVYDLHDGSVVIAAITSCTNTSNPSVMIGAGLMAKKAVEK
                     GLRTRPWVKTSLAPGSKVVTQYLDRAGLTPYLQALGFHLVGYGCTTCIGNSGPLPEAV
                     AETIRRGNLVAASVLSGNRNFEGRIHPLCRANYLASPMLVVAYALAGDVDFDPYTEPL
                     GNDKNGKPVFLHEIWPAPGEITEAVRSAVEPEMFRKEYASVFSGDAAWKKLPVPKSEC
                     FAWEPSSTYVKNPPFFENLPAEPEPVAEIRGARALAVLGDSVTTDHISPAGDIAEDSP
                     AGAYLKNHGIPKEEFNSYGSRRGNHEVMMRGTFANIRLRNLLAPGTEGGWTTYPPGGE
                     KTAIYDAAMLYAKKGTPLIILAGKEYGSGSSRDWAAKGPRLLGVRAVIAESFERIHRS
                     NLVGMGILPLQFVDGATRESLGLTGGEIYAIEGIAGEITPRMRVTVRVSGEGGERTFP
                     ALARIDTPAEVHYYLHGGILPYVLRRLIGKG"
BASE COUNT          281 a          646 c          607 g          336 t
ORIGIN      
        1 cggtccggga ggcgatgcgg acggaggtct ggtccgtcat ccccgacgat tcggtcgagg
       61 acgcgctcct catcctcacc cgggaaaagt tcggcgccct cccggtgctc tccggggacc
      121 gcctggtggg gatcatcacg aaagcggacc tgctgaacgc cttcgtcgaa ctcctcgacg
      181 tgaacggggt ctgcttctgc gtggacgtca ccttcccccg gaacatggtc cggttcgagg
      241 aactgatcga caccttcgcg gggatgggtg tcgaggtccg cagcgtattg atcgctccgc
      301 agggcgagta cggggcgctg aacgcccacc tgcggatcgc cacgatcgac ggcccggcgg
      361 tccgccgggt tctcagtgag aaagggttca tgatcgccga gcaggtttcc cgcctctgaa
      421 cgtcacccct tcccgatcaa ccttcgcagc acgtacggca aaatgccgcc gtggagatag
      481 taatgtacct cggcgggcgt gtcgatccgc gccagcgccg ggaacgtccg ttcgcccccc
      541 tcgccggaaa cgcgcaccgt cacccgcatc cggggcgtga tctccccggc gatcccctcg
      601 atggcataga tttccccccc cgtcaggccg agggactccc tggtggcgcc gtccacgaac
      661 tggagcggga ggattcccat cccgacgaga ttgctgcggt ggatccgctc gaagctctcc
      721 gcgatcaccg cgcgcacccc cagcaaccgc ggccccttgg ccgcccagtc gcgcgacgag
      781 ccggaaccgt actccttccc ggcgaggatg atcagcgggg ttcctttctt ggcgtagagc
      841 atcgccgcgt cgtagatcgc cgttttttcg ccgcccggcg ggtaggtcgt ccacccgccc
      901 tcggttcccg gcgccagcag gttgcgcagc cggatgttgg cgaaggtgcc ccgcatcatc
      961 acttcgtgat ttccccgccg gcttccgtag gagttgaact cctccttcgg gatgccgtgg
     1021 tttttcaggt acgctccggc ggggctgtcc tcggcgatgt ccccggcggg ggagatgtgg
     1081 tctgtcgtca ccgagtcgcc gagcaccgca agagcccgcg cgccgcggat ctccgcgacc
     1141 ggttccggct ccgccggcag gttctcgaag aacggcggat tcttgacgta ggtggaggac
     1201 ggttcccacg cgaagcactc cgacttcggg acggggagct ttttccacgc cgcgtccccg
     1261 gagaagacgg aggcgtactc cttgcggaac atctccggtt cgaccgcgga ccgcaccgcc
     1321 tcggtgatct cccccggcgc cggccagatc tcatggagga aaaccggttt tccgtttttg
     1381 tcgttgccca gcggctcggt gtacgggtcg aagtcgacat ccccggcgag ggcgtacgcc
     1441 accacgagca tcggggaggc gaggtagttc gcccggcaca gcgggtggat ccgtccctcg
     1501 aagttccggt ttcccgacag gaccgacgcg gcgaccaggt ttccgcgccg gatcgtctcc
     1561 gcgaccgcct cggggagggg tccggagtta ccgatgcagg tggtgcagcc gtatccgacg
     1621 aggtggaacc cgagcgcctg cagatacggc gtcagccccg cgcgatcgag gtattgcgtc
     1681 accaccttcg accccggggc gaggctcgtc tttacccacg ggcgcgtccg cagccccttt
     1741 tcgaccgcct tcttcgccat cagccccgcg ccgatcatca cggaggggtt ggaagtgttc
     1801 gtgcagctcg tgatggcggc gatcaccacc gagccgtcgt gcaggtcgta caccccctgg
     1861 tcgagccgga
//
LOCUS       DASVJL010000441         6091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1578200, whole genome shotgun sequence.
ACCESSION   DASVJL010000441 DASVJL010000000
VERSION     DASVJL010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578200"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VF357_09265"
     CDS             <1..754
                     /locus_tag="VF357_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX7524381.1"
                     /translation="ASRMAILRGTLIGFFIGVIPGGGAVISSLVSYAVEKRVSKRPEE
                     FGHGAIEGVAGPESANNAASSSSFIPLLTLGIPGNASIAMIFASLLIKGITPGPFLIV
                     EHPEVFWGVIASMYIGNVMLLVLNLPLVGVWAQLLRVPFGIMAPVIILFTAIGSYSIQ
                     GQVFDIYSLVGFGLFGYLARKLKFEPGPMVLAFVLGPMIEASMRQSLLMSGGSFSIFV
                     TRPISLALLGLFAVLAISQSVVTLRKRFRTTT"
     gene            768..1748
                     /locus_tag="VF357_09270"
     CDS             768..1748
                     /locus_tag="VF357_09270"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7524382.1"
                     /translation="MKRVVAVFAAVMFALVALPAASMAADAYPSKPITAVVPMAAGGS
                     SDLLARAIEKFWQKYSKQPLVVVNKPGGGGVVGTEGVVRSKPDGYTLYLGYGSGHDVV
                     MPSLQKMPYDPLKDLVPVSRISIHSVVIVTGAKSPFKSIKDIVAWSKKEGKPVTTAVS
                     VKAGAVDIALTAFGKAAGINIVTVPFSGGAESTTALAGGHLMIGGGHPSEVIPHIKAG
                     RFKALAVALPQRDPTLPDVPTLKELGYDVATWGSIKGVAAPAGTPKEVVKYIDDNIKK
                     ICNDPEFLKLMTSINQPVMYLGSDDFGKFIKQATADYAKLIKQLNITIED"
     gene            1791..2675
                     /gene="garR"
                     /locus_tag="VF357_09275"
     CDS             1791..2675
                     /gene="garR"
                     /locus_tag="VF357_09275"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: protein motif:HMM:NF008592.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEX7524383.1"
                     /translation="MKTGFIGLGIMGKPMAKNLLKAGYRLVVFDANAAPMKELAAAGA
                     ETAASPKEVAEKCDIVVTMLPNSPHVRAVVLGPQGVIEGAGPGKTVIDMSSIAPLASR
                     EIASLLAEKGVEMLDAPVSGGEPKAIDGTLSVMVGGKKDVFERCYSVMKAMAASVVRV
                     GDTGAGNVAKLANQIVVALNIAAMSEALVFAAKAGVQPDLVYQAIRGGLAGSTVLDAK
                     APLVMDRKFSPGFRINLHIKDLGNVLETSREIGVPLPLTAAVMEMMQALKVDGLGDAD
                     HCSLVRHYEKMAHIEVKR"
     gene            2827..3684
                     /gene="ispE"
                     /locus_tag="VF357_09280"
     CDS             2827..3684
                     /gene="ispE"
                     /locus_tag="VF357_09280"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX7524384.1"
                     /translation="MASAVSFLAPAKLNLSLQVFGKRPDGYHHIRSVMVPVSLYDEVT
                     VEEAPSGICVECDAPGVPTDAANSCHKAAALFLAWAGIPAGVRIRIRKTIPADSGLGG
                     GSSDAAAALKGLIALTGKHPPPEALLEMAIRVGADVPFFLPGGAALVEGIGERLTPLP
                     WNVPFHAVIVRPAFGLSTREGYARLGREPGDPPPRGHVPSFRTISEVAAVVRNDFEAA
                     WVPSHPEIAVIRRELTSAGAAAAGLSGSGSAVFGLFTSEGAAREAREKLSASDDGGKG
                     RRVFVARSV"
     gene            3714..4001
                     /gene="spoVG"
                     /locus_tag="VF357_09285"
     CDS             3714..4001
                     /gene="spoVG"
                     /locus_tag="VF357_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HEX7524385.1"
                     /translation="MRITEVKVFPVSDNEKLKGYATIIFDNCFVVRDLKIIQGSAGLF
                     VAMPSKKTKDGTYRDTAHPLDNETRRMIEEAVIGAYERGTGAVLDGIAAAR"
     gene            4016..4089
                     /locus_tag="VF357_09290"
     tRNA            4016..4089
                     /locus_tag="VF357_09290"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4048..4050,aa:Gln,seq:ttg)
     gene            4187..5128
                     /locus_tag="VF357_09295"
     CDS             4187..5128
                     /locus_tag="VF357_09295"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX7524386.1"
                     /translation="MTRLKIFTGNANPDLAKEICAFLCIPLGSAVIKRFSDGEVNVEI
                     RDNVRGVDVFVIQPTSPPVNDHLMELLILMDGLKRASAKRVTAVLPYYGYARQDRKVL
                     PRAPITAKLVADLLTAAGVSRLLTMDLHAGQIQGFFNIPVDHLYSAPVLLEYIKEKYG
                     NESDIVIVSPDAGGVERARAFAKRLSASLAIIDKRRLAPNVAEVMNIIGEVEGKTAIL
                     LDDMVDTAGTLAQSADALRRKGAKHVYACATHAVLSGPAIDRLEKSEIEELVVTNTIP
                     LGPKAACRKLHVLTVAPLLGEAIKRIHFQDSVSSLFV"
     gene            5142..5840
                     /locus_tag="VF357_09300"
     CDS             5142..5840
                     /locus_tag="VF357_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX7524387.1"
                     /translation="MAMMELTADRRQYTGKEINRKRRAEGKIPGVIYGKGLDTRSIEF
                     QRKPLEKFLDTARRGTVVVKMTVQDGTGGKETYAVLKETQTHPLTGRVTHVDFYEVAL
                     GRKFRVEVPLRIKGKAVGIEMGGVLDVVTRSLEVECTPDHVPEYLELDVASLGIGDSL
                     HLSDVRFPEGVAPMEKDLRMTLAAVHTPKAEAVPVAVEELVAEGAEAASVAEPGAKEE
                     KKEEKPEKESKKKE"
BASE COUNT         1129 a         1866 c         2033 g         1063 t
ORIGIN      
        1 ggcgtccagg atggcgatcc tccgcggcac cctgatcgga ttcttcatcg gcgtgattcc
       61 cggcggcggc gcggtcatct cctccctggt ctcctatgcc gtggaaaaga gggtgtcgaa
      121 acggcccgag gagttcggcc atggcgcgat cgagggggtg gcgggcccgg aatcggcgaa
      181 caacgccgcg tcgagttcgt cgttcatccc tttgctgacc ctcggcattc ccggcaacgc
      241 ctccatcgcg atgatcttcg cctccctgct gatcaaggga atcacccccg ggccgtttct
      301 catcgtggag cacccggaag tcttctgggg agtgatcgct tccatgtaca tcgggaacgt
      361 catgctcctc gtgctgaatc tgccgctcgt gggggtctgg gcccagcttc tccgggtccc
      421 gttcggtatc atggctccgg tcatcattct cttcaccgcc atcggctcgt acagcatcca
      481 gggccaggtg ttcgacatct acagccttgt cggattcggc ctgttcggct acctggcccg
      541 caagctgaag ttcgaacccg gccccatggt gctggccttt gtcctggggc ccatgatcga
      601 agcatcgatg cgccaatccc ttctcatgtc ggggggaagc ttctcgatct tcgtgacgcg
      661 tcccatctcg ctggccctgc tcgggctgtt cgcggttctt gcgataagtc aatccgtcgt
      721 gacgttgcga aagaggttcc ggaccaccac atgaaaggag tagaaaaatg aaaagagtcg
      781 tcgccgtatt cgcagcagtg atgttcgcgt tggtggcgct cccggccgct tcgatggccg
      841 cggacgccta tccatcgaag ccgatcaccg ccgtggtccc catggcggcg ggagggtcct
      901 cggatctcct cgcccgggcc attgaaaagt tctggcaaaa atattcgaaa cagccgctgg
      961 tggtcgtcaa caaacccggc ggcggcgggg tggtcggcac ggaaggggtg gtccgctcca
     1021 agccggacgg ctataccctc tatctcgggt acggttcggg gcacgacgtg gttatgccgt
     1081 ccctccagaa gatgccgtat gatccgttga aagaccttgt ccctgtgtcg aggatctcca
     1141 tccactccgt ggtcatcgtc accggggcga aatccccgtt caaatcgatc aaggacatcg
     1201 tcgcctggtc taaaaaggaa gggaagccgg tcacgacggc ggtatccgtg aaggccggcg
     1261 cggtcgacat cgcgctcaca gccttcggca aggcggcggg gatcaacatc gtcacggttc
     1321 ccttctccgg cggcgccgag tccaccacgg cccttgcggg aggtcacctc atgatcggcg
     1381 gcggccaccc ctctgaggtc attccccaca tcaaggcggg gcgcttcaag gcgctcgcgg
     1441 tggcgctccc ccagcgtgac ccgacccttc ccgacgtccc taccctgaag gagctggggt
     1501 atgacgtggc aacatggggt tccatcaagg gtgtggccgc gcccgccggc acgccgaaag
     1561 aggtggtcaa gtacatcgac gacaacatca agaagatctg caacgaccct gaattcctga
     1621 agctgatgac gagcatcaac cagccggtca tgtatctggg gagcgacgat ttcgggaaat
     1681 tcatcaagca ggcgaccgcg gactacgcca agctgatcaa gcagctgaac atcacgatcg
     1741 aggattgatc cgtcgatcgg ggttgtgagg atgcagaagg agaccaaaca atgaagaccg
     1801 gttttatcgg cctggggatc atgggaaagc cgatggcgaa aaaccttttg aaggcggggt
     1861 accgtctggt ggtgttcgac gccaacgccg cgccgatgaa ggaactagcg gccgccgggg
     1921 ccgaaacggc cgcgtcgccg aaagaggtcg cggagaagtg cgacatcgtc gtgaccatgc
     1981 ttccgaactc tccccatgta agggcggtcg tgttgggtcc ccagggcgtc atcgagggag
     2041 ccggccccgg caagaccgtc atcgacatga gttcgatcgc gccccttgcc agccgggaga
     2101 tcgcttcgct gctggcggaa aaaggggtgg agatgctcga cgcgccagtg agcggcggcg
     2161 agccgaaggc catcgacggg accctctccg tcatggtcgg tgggaagaag gacgtcttcg
     2221 aacggtgcta ctccgtcatg aaggccatgg cggcctccgt cgtgcgggtg ggcgataccg
     2281 gcgcgggcaa cgtcgcgaaa ctggcgaatc agatcgtcgt tgcgctcaat atcgccgcca
     2341 tgtccgaggc gctcgttttc gccgccaagg caggggtaca gccggacctg gtctaccagg
     2401 ccatccgggg gggactggcg ggcagcacgg tgctcgatgc aaaggcgccg ttggtcatgg
     2461 atcgaaaatt ctcccctggt ttccgcatca acctgcatat caaggacctg gggaacgtgc
     2521 tggaaacatc ccgcgagatc ggggtgccgc tcccgttgac cgcggccgta atggagatga
     2581 tgcaggcgct taaggtcgac ggcctcgggg acgcggatca ctgcagcctg gtgcggcact
     2641 acgaaaaaat ggcccacatc gaggtgaaac ggtaagagat ccatcatccg ggaggggcgc
     2701 ccgaagtcgc ccctcccggc cgcatttcgt tcccacaacc caacgtgtgc ccaaaaccca
     2761 ctcttgacat gcgattacca tgcgtatacc ttgatttgaa aacagcgttt gaaaaggtgt
     2821 tccttgatgg cttccgccgt ctccttcctc gctccggcca agttgaatct ctccctgcag
     2881 gtgttcggga aacgccccga cgggtaccac cacatccggt cggtcatggt gccggtctcc
     2941 ctgtacgacg aggtgaccgt cgaggaggct ccctcgggga tctgcgtgga atgcgacgcg
     3001 ccgggggtcc cgaccgacgc cgcgaacagc tgccacaagg ccgccgcact cttcctcgcc
     3061 tgggcgggga tccccgccgg ggttcggatc cggatccgga agacgatccc cgctgattcg
     3121 gggctgggag ggggaagttc cgacgcggcg gcggcgctga aagggttgat cgccctgacc
     3181 gggaagcacc ctcctccgga agcgttgctg gagatggcga tccgcgtcgg ggcagacgtc
     3241 cccttcttcc ttccgggcgg cgcggcgctc gtcgagggga tcggcgagcg gcttaccccc
     3301 ctcccgtgga acgttccgtt tcacgcggtc atcgtgcgcc ccgccttcgg gctgtcgacg
     3361 cgggaggggt acgcgcggct tgggcgagaa ccgggcgacc ccccgcctcg tggccacgtc
     3421 ccgtcgttcc ggacgatttc ggaggtggca gccgttgtcc gaaacgattt cgaggccgcg
     3481 tgggtcccct cgcatccgga gatcgccgtg atccggcggg aactgacgtc cgcgggagcg
     3541 gcggcggcgg ggctgtcggg gagcggatcg gcggtgttcg gcctcttcac gtcggaaggc
     3601 gcggcgcggg aggcgcggga aaaattgtcg gcaagcgacg acgggggaaa agggcgacgg
     3661 gtcttcgtcg cgagaagcgt ctaacaactt tcggggaagg ggaggaggcc aggatgcgga
     3721 tcaccgaggt gaaggtcttt ccggtgtcgg acaacgagaa gttgaaaggg tacgccacga
     3781 tcatcttcga caactgcttc gtcgtccgcg acctgaagat catccagggg agcgccggcc
     3841 tgttcgtcgc catgccgagc aagaagacga aggacggcac gtaccgggac accgctcacc
     3901 ccctggacaa cgagacccga cggatgatcg aggaagccgt gatcggggcg tacgagcggg
     3961 gaaccggcgc cgtccttgac gggatcgcgg ccgcccgcta gaatcgcggg tgcgctggga
     4021 ggtcgtctaa cggcaggacc ggggcctttg aagccccaga tgaaggttcg accccttccc
     4081 tcccagccat gccgggggcg tggtcggaaa gggcttgcca accatcctcc ttaggtataa
     4141 cattcaatcc tttcagcgaa gtttcctccg gatacagggg agttgtgtga cgcgactgaa
     4201 gattttcacc gggaacgcga acccggatct cgcgaaggag atctgcgcgt ttctctgcat
     4261 cccgctgggg tccgcggtca tcaagcggtt cagcgacggg gaggtgaacg tcgagatccg
     4321 ggacaacgtc cgcggcgtgg atgttttcgt gatccagccc acgtctcccc cggtgaacga
     4381 ccacctgatg gagttgctga tcctgatgga cggcctcaag cgcgcctcgg cgaagcgggt
     4441 gacggcggtg ctcccgtatt acggatacgc gcggcaggac cggaaggtcc tccctcgggc
     4501 cccgatcacg gcgaagctcg tggccgacct cctgaccgcg gcgggcgtct cccggctgtt
     4561 gacgatggac cttcacgcgg gacagatcca gggattcttc aacattccgg tggaccatct
     4621 ttactcggcg ccggtgctgc tcgagtacat caaggagaag tacgggaacg agagcgacat
     4681 cgtgatcgtc tccccggacg cagggggcgt cgagcgggcg cgggccttcg ccaagcgcct
     4741 ttccgcctcc ctcgccatca tcgacaaacg ccggctggcg cccaatgtgg ccgaggtgat
     4801 gaacatcatc ggtgaggtcg aggggaagac ggcgatcctc ctcgacgaca tggtggacac
     4861 ggccggcacg ctcgcgcagt ccgccgacgc cctgcgccgc aagggggcga aacacgtcta
     4921 cgcctgcgcc acccacgcgg tcctgtcggg cccggccatc gaccggctcg agaaatcgga
     4981 gatcgaggaa ctcgtggtga ccaacacgat tcccctgggt cccaaggcgg cgtgccggaa
     5041 gcttcacgtc ctcacggtcg cgcccctgct gggcgaggcc atcaagagga tccatttcca
     5101 ggattcggtc agctcgctgt tcgtataaga ggagagaaaa gatggcgatg atggagttga
     5161 cggcggaccg ccgccagtac acggggaagg agatcaaccg gaagcgtcgc gccgagggga
     5221 agatccccgg cgtcatctac ggcaaggggc tcgatacccg gtcgatcgag ttccagcgca
     5281 agcccctcga aaagtttctc gatacggcac gccgcgggac ggtggtcgtc aagatgaccg
     5341 tgcaggacgg gaccgggggg aaagagacgt acgcggtcct gaaagagacc cagacccatc
     5401 ccctgaccgg ccgggtcacc cacgtcgatt tctacgaggt ggcgctcggg aggaagttcc
     5461 gcgtcgaggt cccgctgcgg atcaagggga aggcggtcgg catcgagatg ggtggagtcc
     5521 tcgatgtggt cacccgcagc ctcgaggtag agtgtacgcc cgaccatgtt cccgagtatc
     5581 tcgaactcga tgtcgcctcc ctcggaatcg gagattcgct tcacctttcg gacgtccggt
     5641 tccccgaggg cgtcgccccg atggagaagg atcttcggat gaccctggcg gcggtccaca
     5701 cgccgaaggc cgaggccgtg ccggtggcgg tcgaggagct ggtggcggaa ggggccgagg
     5761 ccgccagcgt ggcggagccc ggagcgaagg aagagaagaa ggaagagaag ccggaaaagg
     5821 aatcgaagaa gaaggagtag cgttccacgc gactcatcgt cggactgggg aatcctgggc
     5881 gtcggtacgg cgccacgctc cacaacgcgg ggttcctggc gatcgaccgg atcgcagaat
     5941 cgctgggtgt ccggtggcgc gcggccgccg cggcttcgct gggaaccggc gagatcgatg
     6001 ggcggcaggt cctcctcgcc aagccgatga cgttcatgaa cctgtccggc gacgcggtgg
     6061 ccccgctcta tcggaagcac gccgacggcc c
//
LOCUS       DASVJL010000442         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_264003, whole genome shotgun sequence.
ACCESSION   DASVJL010000442 DASVJL010000000
VERSION     DASVJL010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264003"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VF357_09305"
     CDS             complement(<1..443)
                     /locus_tag="VF357_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX7524388.1"
                     /translation="MSAKGTGSRVAVVIPARYGATRLPGKPLAEIDGRPMIWYVWSKA
                     SAAKIPSRVVVATDDARIVSVVRGFGGEAVLTSPECASGTDRVAEAARAMDEEILINL
                     QGDEPLMHPSVIDAVAAPLLAEPDVMMSTAALPQVDPGEYARASVV"
     gene            complement(440..1480)
                     /gene="argC"
                     /locus_tag="VF357_09310"
     CDS             complement(440..1480)
                     /gene="argC"
                     /locus_tag="VF357_09310"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX7524389.1"
                     /translation="MKRVAIFGATGYTGFELIRLLLSHTGVKLSVLTSEQYSSQPLGQ
                     AFPPFKGKLAGVSFGKMERLLSAEFDVAFLALPHTLSSAVAGPLLSRGIPVIDLSADF
                     RFRSIPLYESVYGVAHGSPALAAKAVYGLPEIHRAGIRKTRLAAVPGCFPTSVILGLY
                     PILEEGLVERKGIVADCKTGVSGGGRGPALGFHYPEVEGGVRPYGLLNHRHNPEMDQE
                     LSLAAGETVAITFVPHLVPMVRGILATCYAMPKAKVTAKDVEAAYRKRYGKEHFVRLL
                     PPGLLPSTKDVLGSNFCDIAWRVDEKSRRVVVVSAIDNLVKGATGAAVQCFNLMSGFP
                     EEEGLRAMPLFP"
     gene            complement(1633..2025)
                     /gene="rpsI"
                     /locus_tag="VF357_09315"
     CDS             complement(1633..2025)
                     /gene="rpsI"
                     /locus_tag="VF357_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX7524390.1"
                     /translation="MAQARIYATGKRKTAIARVYIKAGAGRITVNGREFEEYFPVLAL
                     RAVATQPLVLTGKRTLVDVDVNIRGGGPMSQAESVKFGIAKALQIENPELRTQLKRAG
                     FLTRDARIKERKKYGQPGARKRFQFSKR"
     gene            complement(2042..2482)
                     /gene="rplM"
                     /locus_tag="VF357_09320"
     CDS             complement(2042..2482)
                     /gene="rplM"
                     /locus_tag="VF357_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX7524391.1"
                     /translation="MKTTKMLTRDSADQTWYVMDAEGKVLGRMATKIADVLRGKHKPT
                     FTPNSDMGDFVIVVNADKVKLTGNKMTGKVYYSHSGYMGGLKSTTPEKVLGSAHPERI
                     VEWAVRGMLPKTRLGDRLFTKLKVYVGPEHPHKAQQPKVLAVNE"
BASE COUNT          476 a          924 c          858 g          518 t
ORIGIN      
        1 accacggagg cgcgcgcgta ttcgcccggg tccacctggg ggagcgccgc cgtcgacatc
       61 atgacgtccg gctcggcgag caggggcgcg gccaccgcat cgatgaccga cgggtgcatc
      121 agcggctcgt ccccctgcag gttgatcagg atctcctcgt ccatcgcgcg ggctgcctcg
      181 gcgacccggt cggttccgga ggcgcattcg ggggaggtga gaaccgcctc gcccccgaac
      241 ccgcgaacca ccgaaacgat ccgcgcgtcg tcggtcgcga ccacgacgcg cgacgggatt
      301 ttcgcggcgc tcgccttgct ccagacatac catatcatcg ggcggccgtc gatttcggcc
      361 agcggttttc ccggcagccg cgtcgcgccg tagcgggcgg gaatcacaac agcgacccgg
      421 ctgcccgtcc ctttcgcgct cacgggaaca ggggcatcgc ccggagcccc tcttcctcgg
      481 ggaatcccga catcaggttg aagcattgca ccgccgcccc ggtcgcgccc ttcacaaggt
      541 tgtcgatggc ggagacgacc accacccggc ggctcttctc gtccacccgc caggcgatgt
      601 cgcagaagtt gcttcccagc acgtctttcg tggatggaag cagccccggc ggcagcaggc
      661 ggacgaaatg ctctttcccg taccgtttcc ggtaggccgc ctccacgtcc ttcgcggtca
      721 ccttggcctt cggcatggcg tagcaggtgg cgaggattcc gcggaccatc gggaccaggt
      781 gcgggacgaa ggtgatcgcc accgtttccc ccgcggcgag cgacagctcc tggtccatct
      841 ccgggttgtg gcgatgattc agcagcccgt acggccggac gcccccttcg acctcggggt
      901 agtgaaagcc gagggcgggc ccccgcccgc ctccggaaac gccggtcttg caatccgcca
      961 cgatcccctt ccgttcgacg agcccttcct cgaggatcgg gtacagtccc aggatcaccg
     1021 aggtggggaa gcagcccggg acggcggcaa ggcgtgtctt ccggatcccc gcacggtgga
     1081 tctcgggcag accgtagacc gccttcgcgg cgagggccgg gctcccgtgg gcgaccccgt
     1141 agaccgattc gtacagcggg atgctgcgga accggaaatc ggccgacagg tcgatcaccg
     1201 gaatgcccct ggacagcagc ggtcccgcga ccgcggacga caaggtgtgc ggcagggcga
     1261 ggaaggcgac gtcgaactcc gcggacagaa gtcgctccat cttcccgaag gagacgccgg
     1321 caagcttccc cttgaacgga gggaacgcct gcccgagcgg ctgcgaggag tattgctccg
     1381 aggtgaggac ggagagcttc acccccgtgt gggacaggag cagccggatc agctcgaaac
     1441 cggtgtaccc ggtcgccccg aagatcgcca cgcgcttcat ccgtttttcc atccttccca
     1501 ttgtataagg tttcgccgcg agatacgccc gccggcttca cccaccggat aaaaaagaag
     1561 aggaaggcgg gttgccccgc cttccccttc gggagatcgt tcgattcggg gcgtctttgc
     1621 cccttcggcg gcttaccgct tggagaactg gaaccgtttc cgcgcccccg gctggccgta
     1681 ctttttccgc tccttgatcc gcgcgtcgcg ggtcaggaac cccgcgcgct tcaactgcgt
     1741 ccgcaactcg gggttctcga tctgcagcgc cttcgcgatg ccgaatttga ccgactcggc
     1801 ctgggacatg gggccgccgc ctcggatgtt cacgtcgacg tcgacgagcg tccgcttccc
     1861 ggtcaggacc aggggctgcg tcgcgaccgc acgaagcgcc agcaccggga agtactcctc
     1921 gaactcccgg ccgttgacgg tgatgcgacc ggcccctgcc ttgatgtaga cgcgggcgat
     1981 cgcggtcttc cgtttccctg tggcgtaaat tctcgcttga gccatggtgc ggtggttcct
     2041 cctattcgtt gacggccaaa accttcggct gctgcgcctt gtgcgggtgt tccggcccca
     2101 catagacctt gagcttggtg aagagccggt cgcccaggcg cgttttcggg agcatcccgc
     2161 ggaccgccca ttccacgatc cgttccgggt gcgcactccc gagcactttc tcgggggtgg
     2221 tggacttgag gccgcccatg tatccggagt ggctgtagta tactttcccc gtcatcttgt
     2281 tcccggtgag cttcaccttg tcggcgttca caacgatgac gaaatcgccc atgtcggagt
     2341 tgggcgtgaa cgtcggcttg tgcttgccgc gcaggacgtc ggcgattttc gtggccattc
     2401 gcccgagaac ctttccctcg gcgtccatga cgtaccacgt ctggtcggca gaatccctgg
     2461 tcagcatctt cgtcgttttc atcgcgcaga ccttcccctc gaaatcaaag gatcatatct
     2521 aacaggattt gccgggccgc gtcaagggga aaaagatgga gggaaggcgc ttgccgcagg
     2581 aacccggccc cgaaagcaac ggccaggaga tcaactatta cgacatctcc tcatcaatat
     2641 ttattctgca acgaggacac cgatcgattt ttccggcgga aaacacgatg tgatatccca
     2701 atatcgcttt acacttcgga cacctgattc cgaggtacat aagtatgttt ccggcaagaa
     2761 acaatgaaat agcgct
//
LOCUS       DASVJL010000443         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_826812, whole genome shotgun sequence.
ACCESSION   DASVJL010000443 DASVJL010000000
VERSION     DASVJL010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826812"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VF357_09325"
     CDS             <1..234
                     /locus_tag="VF357_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524392.1"
                     /translation="AWRGKDPIRRFAAFLKGRGLLEDPAAEEAGAKEIVRGMVEAAEG
                     APPVPLSSLFEDVYAEIPWHLAEQRDSLLSGKV"
     gene            239..1213
                     /locus_tag="VF357_09330"
     CDS             239..1213
                     /locus_tag="VF357_09330"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX7524393.1"
                     /translation="MAEVTLIKAINDALETEMARDPSVCVLGEDVGRGGGVFRATEGL
                     FRRFGPERVIDTPLNEVGIVGMAIGMAMSGLRPVAEIEFSDFIYPAFDQIVSEMAKMR
                     YRSAGQFTAAVVLRAPSGGGIRGGHFHSQSPEAYFIHTAGLVVVMPSTPADAKGLLSS
                     AIRGEDPVIFLEPKALYRTVKEEVPEGEHLVPLGKGRIVRPGEDVTLVTWGAMVPVAS
                     MAAATAASEGVDVEIIDPRTLWPLDIEMIEASVKRTGRAVVLHEAPRTCGFGAEVSAL
                     IQERCFLHLKAPVARVTGFDTPFPFALEKSYLPDPERTLRAIRASMDF"
     gene            1318..>1764
                     /locus_tag="VF357_09335"
     CDS             1318..>1764
                     /locus_tag="VF357_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7524394.1"
                     /translation="MKKKGMNRRDFLKLTGAGSLAAAAAGGLGGGLLTPKWVWGATPS
                     VKGPIKIGYQAVLSGTLAGYGEFHKMGAVMAIEEINAKGGIAGQKVEMEFRDSTTKAD
                     EAIKNARYFVDSWGVDFLAGIDSSGQALALAPVMAQLDRVLMVTHGA"
BASE COUNT          289 a          526 c          669 g          280 t
ORIGIN      
        1 gcatggcgcg ggaaggaccc gatccggcgg ttcgccgcgt tcctcaaggg gcggggcctg
       61 ctcgaggacc cggccgcgga ggaggcggga gcgaaggaga tcgtacgcgg gatggtcgag
      121 gcggcggagg gcgcgccccc cgtgccgctg tcgagcctgt tcgaggacgt ctacgcggaa
      181 atcccatggc acctcgccga gcagcgcgac tccctgctct ccggaaaggt gtgaggcgat
      241 ggccgaggtg actctgatca aggcgatcaa cgacgcgctc gagacggaga tggcgcggga
      301 cccgtccgtc tgcgtcctcg gagaggacgt cggccgcggc ggcggggtct tccgggcgac
      361 ggaaggattg ttccgccggt tcgggccgga gcgggtgatc gacacgccgc tgaacgaggt
      421 cgggatcgtc gggatggcga tcgggatggc gatgagcggc ctgcgaccgg tcgccgagat
      481 cgagttctcc gatttcatct acccggcgtt cgaccagatc gtctcggaga tggccaagat
      541 gcggtaccgc tccgcggggc agttcacggc ggcggtcgtg ctgcgcgccc cctcgggcgg
      601 ggggatccgg ggagggcact tccactccca gagcccggag gcgtacttca tccacaccgc
      661 ggggctggtc gtcgtgatgc cgtcgacgcc ggccgacgcg aaggggctcc tctcctcggc
      721 gatccgtggg gaagatccgg tgatcttcct cgagccgaag gcgctctacc ggacggtaaa
      781 ggaggaggtg ccggaggggg agcacctcgt tccgctcggg aagggacgga tcgtccggcc
      841 cggggaggac gtgacgctgg tgacgtgggg cgcgatggtg ccggtcgctt cgatggcggc
      901 cgctaccgct gcgtccgagg gggttgacgt cgagatcatc gatccgcgca ccttgtggcc
      961 gctggacatc gagatgatcg aggcgtcggt gaagcggacg ggccgggccg tcgtcctcca
     1021 cgaggcgccc cggacgtgcg gcttcggcgc ggaggtctcc gcgctcatcc aggaacggtg
     1081 cttcctgcac ctgaaggcgc ccgtcgcgcg agtgacgggg ttcgacaccc cgtttccctt
     1141 cgccctcgaa aagtcgtacc tgccggaccc ggagagaacg ctgcgggcga ttcgcgcgtc
     1201 gatggatttt tgagaaatcc ccgatgtatg atacgaggat cattatcgcc gtcaatgcgt
     1261 cgaggcatcg cttgaggcat cggtgcattc acgagatcat ccattgaggg aggagcgatg
     1321 aaaaaaaagg gtatgaaccg acgggatttc ctgaagctca cgggggccgg ttccctggct
     1381 gcggcggcgg caggggggct gggcggcggc ctgctcaccc cgaagtgggt gtggggggcc
     1441 acgccttcgg tcaaggggcc gatcaagatc ggctaccagg cggtcctctc cgggacgctc
     1501 gccgggtacg gggagttcca caagatggga gcggtcatgg ccatcgagga gatcaacgcg
     1561 aaggggggga tcgcagggca gaaggtcgaa atggagttcc gcgactcgac cacaaaagcc
     1621 gacgaagcga tcaagaacgc ccgctatttc gtcgatagct ggggcgtgga cttcctcgcc
     1681 ggcatcgact cctcggggca ggcgctggcg ctggcgcccg tgatggccca gctcgaccgg
     1741 gtcctgatgg tgacccacgg cgcg
//
LOCUS       DASVJL010000444         3615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_171730, whole genome shotgun sequence.
ACCESSION   DASVJL010000444 DASVJL010000000
VERSION     DASVJL010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171730"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /gene="ruvB"
                     /locus_tag="VF357_09340"
     CDS             complement(<1..962)
                     /gene="ruvB"
                     /locus_tag="VF357_09340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX7524395.1"
                     /translation="MPGGRIVDPSAAGGENPVDLSLRPKRFGEFIGQAGIVANLRTYI
                     EAAIGRGEPLDHVLLSGPPGLGKTTLAHIIANEMGAGIRTTSGPAIERKGDIAAILTA
                     LEPGDVLFIDEIHRLARVVEELLYSAMEDFALDIILGQGPSAKSIRLALPRFTLVGAT
                     TRTGLLTSPLRDRFGVPLRLEYYGTEELKEVIRRSSAALSIPVDEAGAGEIARRSRGT
                     PRVANRLLRRVRDFAQVTGDGTITREIADHALLRMEVDREGLDVMDRKILRVLLEKFG
                     GGPVGVETISASVSEERDTIEDVYEPFLIQRGFLKRTPRGRVATP"
     gene            complement(993..1604)
                     /gene="ruvA"
                     /locus_tag="VF357_09345"
     CDS             complement(993..1604)
                     /gene="ruvA"
                     /locus_tag="VF357_09345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX7524396.1"
                     /translation="MIDHLRGRLAGGGKDFVVLECAGIGFRARVSDATRKDLPPDGES
                     CILRTHLHFREGGADLYGFSTDTEREIFLATIGVSGVGPKSAMAMLSVLGLPGVLAAC
                     AREEAAAFSRVPGIGKKLAQRIALELPDRLKKVSVDFAPLGGEAADLPTAPEAQASEA
                     LASLGFPRAQAQLAVAAVRREKGADLPTDLLIRESLRRLSGGR"
     gene            complement(1601..2131)
                     /gene="ruvC"
                     /locus_tag="VF357_09350"
     CDS             complement(1601..2131)
                     /gene="ruvC"
                     /locus_tag="VF357_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX7524397.1"
                     /translation="MGELRHLRRGYGGVRLRRILGIDPGSLRTGYGIVDVRGSAVSPV
                     AWGVIRLEGEASFPDRLFRIHRELSDLILLHRPTEAAVEKVFLAKNPSSALKLGQARG
                     AAIVTCGAHGVAVHEYSAKEIKAAATGYGGAPKEQVSGMVARLLGIRDPIPPDASDAL
                     AMAFCRAVTRVVSYPR"
     gene            complement(2083..2835)
                     /locus_tag="VF357_09355"
     CDS             complement(2083..2835)
                     /locus_tag="VF357_09355"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7524398.1"
                     /translation="MSGHNKWSSIKHKKGKADALRGRAFTKITREIITAAKIGGGDPE
                     GNSRLRAAILAAKAVNMPNDNISRAIKKGTGELEGVSYEEYMYEGYGPNGVAVLVKVL
                     TDNKNRTVADLRHTFTKYNGNLGETGCVNWMFGKKGSISVLKESVSEEKLMELALDLG
                     ADDVDNDSESHEFEVRCEPEVFEDVKKGLEEKGVKISTAEVAMVPQTTVHLEGKPAET
                     TLKMMNALEESDDVQNVWANFDISDEDMEAFG"
     gene            complement(3527..>3615)
                     /gene="rrf"
                     /locus_tag="VF357_09360"
     rRNA            complement(3527..>3615)
                     /gene="rrf"
                     /locus_tag="VF357_09360"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          623 a         1200 c         1160 g          632 t
ORIGIN      
        1 ggggtggcca cccttccccg cggagtgcgc ttgaggaaac cccgctggat gagaaacggc
       61 tcgtagacat cctcgatcgt gtcccgctcc tcgctgacgg aggcggagat cgtctccacg
      121 ccgacggggc ctcccccgaa cttctcgagg agcacgcgca ggatcttccg atccatcacg
      181 tcgagcccct cccggtccac ctccatccgc agcagcgcat gatccgcgat ttcccgggtg
      241 atcgtgccgt cccccgtcac ctgggcgaag tcgcggacgc gccgcaggag gcggttcgcg
      301 acgcgcggcg ttccccggga gcgacgggcg atctctcccg cccccgcctc gtcgacgggg
      361 atggagaggg cggccgagga gcgccggatc acctccttca gttcctccgt cccgtaatac
      421 tccagacgca gggggacccc aaaccggtcc cgcaacgggg aagtgagcag cccggtgcgc
      481 gtcgttgcgc cgacgagtgt gaacctcgga agcgcgaggc ggatcgattt cgcggagggc
      541 ccctgcccga ggatgatgtc gagggcgaag tcctccatcg cggagtagag caattcctcg
      601 accacgcgcg ccagccggtg gatctcgtcg atgaagagga cgtccccggg ctccagcgcc
      661 gtgagaatcg cggcgatgtc ccctttccgc tcgatcgcgg ggccggaggt cgtgcggatc
      721 cccgctccca tctcgttggc gatgatgtga gccagcgtcg tcttcccgag gcccggcggg
      781 ccggagagga ggacgtggtc gagcggctcg ccgcgaccga tcgccgcctc gatgtaggtc
      841 cgaaggttcg ccacgatgcc ggcttgcccg atgaactcgc cgaaccgctt cggccgcagg
      901 gagaggtcga cggggttctc gccgccggcg gcggagggat cgacgatgcg tccacccggc
      961 atttccgcgt ctctccgttc cgtcgtcacg cgttaccgcc ctcccgacag gcgacgcaac
     1021 gactcccgga tcaggagatc cgtggggaga tcggcgcctt tctcccgccg gacggccgcg
     1081 acggcaagct gggcctgcgc ccgcggaaac ccgagggaag cgagcgcctc cgacgcctgc
     1141 gcttccggcg ccgtggggag atccgccgcc tccccgccga ggggggcgaa atcgaccgaa
     1201 accttcttca gccggtccgg cagctccagg gcgatcctct gggcgagctt cttcccgatc
     1261 cccggcacgc gcgagaaggc ggcggcctct tcccgcgcgc acgccgcgag aacgccagga
     1321 aggccgagga ccgacaacat cgccatggcg gatttcggtc ccacgccgct caccccgatc
     1381 gtcgcgagaa agatctcgcg ctccgtgtcc gtggagaaac cgtacaggtc ggcgccgccc
     1441 tcccggaaat ggagatgcgt gcggaggatg cagctctcac cgtcgggggg gaggtccttg
     1501 cgggtcgcgt cggagacgcg cgcgcggaat ccgatcccgg cgcactccag caccacgaaa
     1561 tccttcccgc cccccgcgag gcgcccgcgc agatgatcta tcatcgcggg tatgatacca
     1621 cgcgggtgac ggcgcggcag aaggccatgg cgagggcgtc ggaggcgtcg ggagggatcg
     1681 ggtcgcggat gccgaggagg cgggcgacca tcccggagac ctgctccttg ggggcgccgc
     1741 cgtatccggt cgccgccgcc ttgatctcct tggcgctgta ttcgtggacg gcgacgccgt
     1801 gggcgccgca ggtgacgatc gcggcgccgc gcgcctggcc gagtttcagg gcggaggacg
     1861 gatttttcgc gaggaagact ttttcgaccg cggcttccgt gggacgatgg agaaggatca
     1921 ggtcggaaag ctcgcgatgg atcctgaaga gacggtcggg gaaggacgcc tcgccttcga
     1981 gccggatgac gccccacgcc acgggggaga ccgcgctccc ccggacgtcg acgatcccgt
     2041 acccggtccg cagcgaccct gggtcgatgc ccagaatccg cctcagccga acgcctccat
     2101 atcctcgtcg gagatgtcga agttcgccca gacgttctgg acgtcgtccg actcctcgag
     2161 ggcgttcatc atcttgagcg tcgtttcggc gggcttcccc tcgagatgga ccgtggtctg
     2221 ggggaccatc gccacctcgg ccgtggagat cttgacgccc ttctcctcca ggcccttctt
     2281 cacgtcctcg aagacctccg gctcgcaccg gacctcgaac tcatgcgatt cggaatcgtt
     2341 gtcgacgtcg tcggcaccga ggtcgagggc gagttccatc agcttctctt cactgacgga
     2401 ctccttgagg acgctgatcg atcctttctt gccgaacatc cagttgacgc accccgtctc
     2461 gccgaggttg ccgttgtatt tcgtgaacgt gtgccgaaga tcggcgacgg tgcggttctt
     2521 gttgtcggtg aggaccttca ccagcaccgc caccccgttc ggcccgtacc cttcgtacat
     2581 gtactcctcg tacgagacgc cttcgagctc gccggtcccc ttcttgatcg cccgggagat
     2641 gttgtcgttg ggcatgttga ccgccttggc ggccaggatc gcggcccgga gacgggaatt
     2701 cccctccggg tcgccgccgc cgatcttcgc cgcggtgatg atctcgcggg tgatcttggt
     2761 gaacgccctg cccctgaggg cgtcggcctt ccctttcttg tgcttgatcg agctccattt
     2821 attgtgtccg gacatggttc ctctaccttt acgatcattt gggccgtaga gtatcggcga
     2881 tgggatgttc gtgaaccgaa agtatagcac aggcggaaac ggttgcgtcg gtagatgatg
     2941 aggaggcgga aacggatcaa gcggtaccga tcggctccgc gcgccggcag gaacctgatc
     3001 ggcggtttac acgaaaatgt ccacgataag accgctgggg tacaggcatt cgcccaggtg
     3061 ggcatggctg gcccgcatcc gttccttcga attgccgaga agatccggcc gcgagttcag
     3121 gggatggcac accgtcgcat ccgccggaac cgcccccgga gggcggaacg gcggccttcc
     3181 cgcacagggt cgaagagcca acatccgttc tccggcccgc atcggcacga tcgaagtaac
     3241 catcgaagta accacgtgta cgctcccccg atgccggcaa cccacggcaa taaaaaccac
     3301 gaaagggacg accgaattct atccatatat aatgcaacac gcataccaat ccgtaagtaa
     3361 ttgttcgtac taagaaaata tacgacggcc ccctgaaacc tgggtatcga atgtcccatt
     3421 ttcggtaaat tatttccgag aatgatctcc gaacaaggcg ggaccgatcc tatcgccgat
     3481 aaaaaaaggc ccctgctccg tttccggcgc aagggcccaa gattaaattc ggcggcgacc
     3541 tactctccca cgggggtaac cccgcagtac catcggcgct ggagagctta actgccgtgt
     3601 tcgggatggg aacgg
//
LOCUS       DASVJL010000445         4096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1296946, whole genome shotgun sequence.
ACCESSION   DASVJL010000445 DASVJL010000000
VERSION     DASVJL010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296946"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VF357_09365"
     CDS             <1..455
                     /locus_tag="VF357_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524399.1"
                     /translation="WYAEYAGLPEDIIQAIRHHHHPSGATGNMLGVSVVALAEVFSRR
                     FSPRFIDDGLWTPEHDAIMLELDTGEDSMQTICHKLGGLKYDIKLFFGVEGGRTMPIE
                     ELKSIFQGVTIRREKDADASRRGKKRPPPPKKGDEDPDSDKGTVDITA"
     gene            465..821
                     /locus_tag="VF357_09370"
     CDS             465..821
                     /locus_tag="VF357_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524400.1"
                     /translation="MLKGLFKRKRSTESDDARERLTLELVEIEEIAGILFKRMEARLE
                     EVKAIEGRLDEKASALESLLSRAASIGFAHSDAVDARRREVVALSRKGLKVDEIAGVF
                     DMTRGEVELILNLGDK"
     gene            971..1681
                     /locus_tag="VF357_09375"
     CDS             971..1681
                     /locus_tag="VF357_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HEX7524401.1"
                     /translation="MHKGIYIALSGSVLKQDQIDIVSNNIANANTVGFKKERASFKEF
                     LVSQISGAKDSDDGRAMSDLSTMKIDFRAGTHLKTGNPLDIALDGQGFLSIEGGRYTR
                     KGDLRVGRDGFLVTQEGLKVLGSKGPIKLAAGQADIAPSGEVRVNGKPVDTLKIVDFA
                     NQGELTELEGGYYTANLPSIRSNAIVSPGHLEASNVDAVKEMVQMITTLREFETYQKA
                     IQMFDEAAARVNNDMAKV"
     gene            1723..2508
                     /gene="flgG"
                     /locus_tag="VF357_09380"
     CDS             1723..2508
                     /gene="flgG"
                     /locus_tag="VF357_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEX7524402.1"
                     /translation="MRSLYTAATGMEAQRLNIDVVANNLANVNTTGFKRSRADFQDLL
                     YQDEKTAGAAAAEGLQIPSGIQVGLGVRPVAVQKIFAQGDFASTGNPLDLVIEGGGFF
                     QVPKPDGELAYTRAGSFKLNSDGRIVTSDGYPLEPTITIPTNTTSITVGTDGKITVTQ
                     PGNNAPTEVGRITLARFSNPAGLKSVGRNLYTPTDSSGEAITGNPGTDGMGTIGQGFL
                     ELSNVNVVEEMVNMIASQRAYEINSKAVQASDEMLQVVNNLKR"
     gene            2505..3224
                     /gene="flgA"
                     /locus_tag="VF357_09385"
     CDS             2505..3224
                     /gene="flgA"
                     /locus_tag="VF357_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEX7524403.1"
                     /translation="MMEPKGDRHTPPKGALFRAARSFLVLSAAVLAVYAPSAVHAGGP
                     APAEILRTYVLEHRPWADVEVRNLALSTDPPAGIPRRIVVERGLPGRTVFAMEYGNGI
                     TIKATADVAAFEEVVASARPLSKEHPLKEGDVYLVRMDVENIPSGAVMDLQAVVGKTL
                     TRQIGANLPIVDRYLAGSPLVKRGGKVTLLVEGGGIRITTTGETRDNAYVNASVRAVN
                     LASRKTVTGILIDENTVRVDY"
     gene            3211..3906
                     /locus_tag="VF357_09390"
     CDS             3211..3906
                     /locus_tag="VF357_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEX7524404.1"
                     /translation="MSIIRGACAALGAALVLAGCATAPKLPPPPPKYVYQQDKPNDGR
                     TANSLWSDGGGLVEDFRARRINDLLTIIVSENISGSGAADTNAARTSTLDASVTGFFG
                     APLNLGLANLYGKGNTFSPTVSGSMGDDFKGTGSTNRTGKIVGTITARVTEVLPNGTL
                     SVESRKEITINNEKQVLTMRGLVRPEDISVSNMVASSRVADAEIFLVGDGVLQEKQRQ
                     GWLVRILDSVWPF"
BASE COUNT          841 a         1284 c         1328 g          643 t
ORIGIN      
        1 ggtggtacgc cgaatacgcg ggtctcccgg aggacatcat tcaggcgatc cggcaccacc
       61 accacccatc gggtgcgacg ggaaacatgc tcggcgtttc cgtcgtggcg ctcgccgagg
      121 tgttctcgcg gcgcttctcc ccccggttca tcgacgacgg cctctggacg cccgagcacg
      181 acgccatcat gctcgagctc gacaccgggg aggattccat gcagacgatc tgccataagc
      241 tcggcggcct caagtatgat ataaaattat tcttcggggt ggagggaggt cggacgatgc
      301 cgatcgaaga gctcaagagc atctttcagg gcgtcacgat ccggagagaa aaggacgccg
      361 acgcctcccg ccgcgggaaa aaacggccgc ccccaccgaa gaagggagac gaagatcccg
      421 actccgacaa ggggacggtc gacatcacgg cctaagagga tccgatgctg aagggcctgt
      481 tcaagcggaa gaggagcacc gagtccgacg acgcccggga gcgtcttacg ctcgaactcg
      541 tcgagatcga ggaaatcgcc gggatcctct tcaagaggat ggaggcgcgg ctcgaggaag
      601 tcaaggcgat cgagggacgg ctcgacgaga aggcaagcgc gctggaatcg ctcctttcgc
      661 gcgccgcaag catcgggttt gcccacagcg acgcggtcga cgcgcgccgt cgggaagtcg
      721 tcgccctgtc ccgaaagggt ctgaaagttg acgagatcgc cggtgttttt gacatgaccc
      781 gcggcgaggt ggagctcatc ttgaacctcg gcgacaaata gacgtctttc ccgcggtaaa
      841 atctttcctg ctccggaaaa cttttcctcc ttcgagaggt ttcaccctcc cccactcccc
      901 gcaaacggcc tccgggcacg ctttttccga caattagcgg tttggcatca agattgctgt
      961 atcaggaggc atgcacaagg gtatctacat cgcgctgtcc ggttcggtcc tcaagcagga
     1021 ccagatcgat atcgtttcca acaacatcgc caacgcgaac acggtcggct tcaagaagga
     1081 gcgggcatcg ttcaaggagt tcctcgtttc gcagatcagc ggggcgaagg acagcgacga
     1141 cggccgggcg atgagcgacc tgtccacgat gaagatcgat ttccgggcgg gaacccacct
     1201 caagaccggg aacccgctcg acatcgccct cgacgggcaa ggatttctga gcatagaagg
     1261 cgggcgctac acgaggaagg gtgacctgcg cgtcggtcgg gacggtttcc tcgtcacgca
     1321 ggaggggcta aaggtcctcg ggtccaaggg gcccatcaag ctcgccgccg gccaggccga
     1381 catcgccccg tccggcgaag tgcgcgtcaa cggaaagccg gtcgacaccc tcaagatcgt
     1441 cgacttcgcg aaccagggcg agctcacgga actcgaaggc gggtactaca ccgcgaacct
     1501 cccgtcgata cggtcgaacg ccatcgtgtc cccggggcac ctcgaagcgt ccaacgtcga
     1561 cgcggtgaag gaaatggttc agatgatcac gaccctccgg gagttcgaaa cgtaccagaa
     1621 ggcgatccag atgttcgacg aagcggcggc gcgggtcaac aacgatatgg cgaaggtctg
     1681 agggccggcc gttccaacgg caacgaaggg ggctaagcaa tgatgagatc actctatacc
     1741 gcggcgacgg gaatggaagc gcagcggctc aacatcgacg tggtggcgaa caacctcgcc
     1801 aacgtgaaca ccaccgggtt caagcgctcc cgggccgatt tccaggacct cctctaccag
     1861 gacgaaaaaa cggcgggggc cgccgccgcc gaagggcttc agatcccttc cggcatccag
     1921 gtggggctcg gtgtgcgccc cgtggccgta cagaaaatct tcgcgcaggg cgacttcgca
     1981 agcacgggaa acccgctgga cctcgtcatc gagggcggcg gtttcttcca ggttccgaag
     2041 ccggacggcg agctcgccta tacccgtgcg ggctccttca agctcaacag cgatggtcgt
     2101 atcgtgacct cggacggcta ccccttggag ccgaccatca cgatcccgac caacacgacg
     2161 agcatcaccg tcgggacgga cgggaagatc acggtgacgc agcctgggaa caatgccccg
     2221 acggaagtcg gccgcatcac gctcgcccgc ttctcgaacc ccgcaggcct caagtccgtc
     2281 ggccggaacc tttacacccc gaccgattcg tccggggagg cgatcacagg gaaccccggt
     2341 acggacggga tgggcacgat cggccaggga ttcctcgaat tgagcaacgt gaacgtcgtc
     2401 gaggagatgg tcaacatgat cgcgagccag cgggcctacg agatcaattc gaaagcggtc
     2461 caggcttccg acgagatgct ccaggtcgtc aacaacctga agagatgatg gaacccaagg
     2521 gggatcggca tacgcccccg aaaggggcgc tcttccgcgc ggcgcggtcc ttcctcgtcc
     2581 tttccgcggc ggtcctggcg gtatacgctc cttccgcggt gcatgcgggc ggtcccgcgc
     2641 cggcggagat cctccggacg tacgtgctcg agcaccggcc gtgggccgat gtcgaagtac
     2701 gcaacctggc cctgagcacg gacccgccgg cgggcatccc gcgcaggatc gtcgtggaga
     2761 gggggctacc cggccgaacc gtcttcgcaa tggagtacgg caacggcatc accatcaagg
     2821 caacggccga cgtcgccgcg ttcgaggaag tcgtcgcgag cgcacggccc ctttcgaaag
     2881 agcaccccct caaggagggg gacgtgtacc tggtgcggat ggatgtggag aacatccctt
     2941 ccggcgccgt catggatctg caggcggtcg tcgggaaaac gttgacgcgg cagatcgggg
     3001 cgaaccttcc gatcgttgac cgttacctcg ccgggtctcc gctcgtgaag cgcggcggga
     3061 aggtgaccct cctcgtggag ggcggcggga tccgtatcac caccaccggg gagacacgcg
     3121 acaacgccta cgtcaacgca tccgtaagag cggtcaacct tgcatcaagg aaaacggtca
     3181 cggggatcct gatcgatgag aacacggtcc gtgtcgatta ttaggggggc ttgcgccgcc
     3241 ctgggcgccg cgctggtcct tgcgggctgc gcaacggcgc cgaagctgcc gccgccgccg
     3301 ccgaagtacg tctaccagca ggacaagccg aacgacgggc gtacggccaa ctccctctgg
     3361 agcgacggcg gagggctcgt cgaggacttc cgggcgaggc ggatcaacga ccttctcacc
     3421 atcatcgtat ccgagaacat ctccgggtcc ggcgccgccg acacgaacgc cgcccgcacg
     3481 tccaccctcg acgcgagcgt cacggggttc ttcggcgctc cgctgaatct cggcctggcg
     3541 aacctgtacg gcaaggggaa cacgttctct cccaccgtga gcggttcgat gggggacgac
     3601 ttcaagggaa cggggagcac gaaccggacc gggaaaatcg tcggcacgat cacggcccgc
     3661 gtcacggaag tgctgccgaa cgggaccctc tccgtcgagt cgcgcaagga aatcaccatc
     3721 aacaacgaga agcaggtgct caccatgcgc ggattggtgc ggcccgagga catttccgtg
     3781 tcgaacatgg tcgcgagcag cagggtagca gacgccgaga tcttccttgt cggcgacggc
     3841 gtcctccagg agaagcagcg gcaaggttgg ctcgtgcgga ttctcgacag cgtctggcca
     3901 ttctaggccg aaacggggca ggtgcggcga tgataacgga tggaaatact tcataccgga
     3961 ggggcgtgcc gggcctgacg cgtgtcctgg tgatcctcgg cacggcggcc gttctgcttt
     4021 gcggagcggg cgtcccgtcc cgcgccgagc ggatcaagga cctcgcggcc ttcgaggggg
     4081 tgcgggagaa ccagct
//
LOCUS       DASVJL010000446         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_358967, whole genome shotgun sequence.
ACCESSION   DASVJL010000446 DASVJL010000000
VERSION     DASVJL010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358967"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VF357_09395"
     CDS             <1..626
                     /locus_tag="VF357_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7524405.1"
                     /translation="LIDTAGIRAKRKTEGAVEIFSVMKSLDSIKRCDLAILLIDGPGG
                     LTHQDRQVLRYILDEERAVVVAANKADAWTTEETRRKGLHTITEGLEYAAFAPVIPTV
                     ATSGKGFQQLFRKIEEASANFRLRVPTGLLNRMAQSFLYTVPIPSPTGRNRAFYMTQI
                     GVAPPSFAIFVKNRRGIPDSFTRYLQNKIRDRFGFEGSPVRIVYRER"
     gene            623..1516
                     /locus_tag="VF357_09400"
     CDS             623..1516
                     /locus_tag="VF357_09400"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX7524406.1"
                     /translation="MIRRASTAFQVVTEGAAIFFRNHGPVYSAAIAFNILFSAVPILF
                     LVFAATGWIIGKSELPFAQLSGILRSTFPYGARVLVPNLRHMMEAGTTFGIFGTVLLL
                     FTSFSATDAIHTSLSVMLMRKRQKRILRPLGFHVVFVIVLIVLTAAAIVVPPLWDGIF
                     YLTKGMSVQVDYAFRGILQLVADILLVDIMLLGSVLSYRYLSPGKVRLRNAFIGTVIF
                     LALLQGIRVGFIFYIRKFSKLNLLYGSLFSIICFILVAYLFAAAYLYGASVIGVLERT
                     GREGSIPEKEEGDPVATTCGD"
     gene            1494..2417
                     /locus_tag="VF357_09405"
     CDS             1494..2417
                     /locus_tag="VF357_09405"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524407.1"
                     /translation="MRRLAAIDIGTNTIRMLVAERGGRRPVPVARRRSIVGLGRCLRE
                     TGGIGEAEFAAGLRVLREFRREMRGGEVEAYRACGTACLREAENRADFLEAAAGEGID
                     IEVIGPAEEARLTWEGIRWAVAGRPGDVAMDIGGGSTEFAFGPREGESVSLSTGVVVL
                     STLLPLSDPPHPWELRAVSHYAVGRIEDGTRPFGGRRFRRLIGTAGTFTTLAAMERGM
                     TRYDPDRINGARLSAAVVRRWADRLGGMTDAQRRRLPGMEKGRERYVVPGALLIVAAL
                     ERFRLNGVTVSDAGSLEGILEGIGRNGGEDG"
BASE COUNT          418 a          749 c          884 g          460 t
ORIGIN      
        1 tgctgatcga caccgcgggg atccgcgcga agcggaagac cgaaggggcg gtcgaaatct
       61 tctccgtgat gaagagcctc gactcgatca agcggtgcga cctcgcgatc ctgctgatcg
      121 acggacccgg ggggctcacc catcaggacc ggcaggtcct gcgttatatc ctcgacgagg
      181 agcgcgccgt ggtcgtcgcc gcgaacaagg cagacgcgtg gaccacggag gagacgcgcc
      241 ggaagggatt gcacacgatc acggaagggc tcgaatacgc cgccttcgct cccgtcatcc
      301 ccacggtggc cacatccgga aaaggattcc agcagctctt ccggaagatc gaggaggcca
      361 gcgcgaactt ccgcctgcgc gtcccgaccg gcctgctgaa ccggatggcc cagtcgttcc
      421 tctacaccgt cccgatcccc tccccgacgg ggagaaaccg cgctttttac atgacgcaga
      481 tcggggtcgc gccgccctcc ttcgccattt tcgtgaagaa ccgtcggggg atcccggact
      541 ccttcacccg ctacctgcaa aacaagatcc gcgaccgctt cggattcgaa gggtcgccgg
      601 tccgcatcgt ctaccgggag cgatgattcg tcgggcgtcg acggcgttcc aggtcgttac
      661 ggagggagcg gcgatcttct tccggaacca cggtccggtc tacagcgccg ccatcgcgtt
      721 caacatactg ttctccgcgg tcccgatcct gttcctcgtt ttcgcggcca ccggctggat
      781 catcgggaag agcgaacttc cgttcgcgca gctctcgggg atcctgcgca gcacgtttcc
      841 gtacggagca cgggtactcg tgccgaacct tcggcacatg atggaggcgg ggaccacctt
      901 cggaattttc ggaacggtgc tgctcctctt cacctccttc tccgcgacgg acgcgatcca
      961 tacgtcgctc tcggtgatgc tgatgcgcaa gcgccagaag cggatccttc gcccgctcgg
     1021 cttccacgtc gtcttcgtga tcgtcctcat cgtgctgacc gccgcggcga tcgtcgtgcc
     1081 gccgctgtgg gatgggatct tctacctcac gaaggggatg tccgtgcagg tggactatgc
     1141 gttccggggc atcctgcaac tggtcgcgga tatactcttg gtggacatca tgctgctggg
     1201 gagcgtgctg agctaccggt acctttcccc cggaaaggtg cggctgcgca acgcgttcat
     1261 cgggaccgtg atcttcctcg cgctgctgca aggcatcagg gtgggcttca tcttttacat
     1321 caggaaattc agcaagttga atcttcttta cggttccttg ttcagcatca tttgttttat
     1381 actcgttgcc tacctctttg cggcggcgta tctgtacggg gccagcgtca tcggggttct
     1441 ggagcgtacg ggcagggagg ggtccatccc ggagaaggag gaaggcgacc cggttgcgac
     1501 gacttgcggc gattgacatc gggacgaaca cgatccggat gctcgtcgcg gaacgcggcg
     1561 gaaggcggcc cgtccccgtc gcgcggcggc gatcgatcgt cgggctggga cgctgcttga
     1621 gggaaaccgg cgggatcgga gaggcggagt tcgcggcggg cctccgggtc cttcgggaat
     1681 ttcgccggga gatgcggggc ggggaggttg aggcgtaccg ggcgtgcggc accgcctgcc
     1741 tgcgagaggc cgaaaaccgc gccgacttcc tcgaggcggc cgcgggcgag gggatcgaca
     1801 tcgaggtgat cggcccggcc gaggaggcgc gcctcacctg ggagggaatc cgttgggcgg
     1861 tcgccggaag gccgggagac gtggcgatgg acatcggcgg gggaagcacc gagttcgcct
     1921 tcgggccccg ggagggtgaa tcggtttccc tttcgacggg ggtggtcgtc ctctccaccc
     1981 ttctgccgct gtcggatcct ccgcatccgt gggagcttcg cgccgtgtca cattacgcgg
     2041 tggggaggat cgaggacggg acgcggccgt tcgggggcag gagatttcgc cggttgatcg
     2101 gcacggcggg aacgttcacg acgctggcag cgatggagcg gggaatgacc cgctacgatc
     2161 cggaccggat caacggtgcg aggctttccg ctgccgttgt ccgacggtgg gccgaccggc
     2221 ttggggggat gacggacgca cagcggaggc gcctgccggg gatggagaag ggacgggaac
     2281 ggtacgtcgt tcccggggcg ctgctgatcg tcgcggcgct ggagcgtttc cgtttgaacg
     2341 gggtgacggt gagcgacgca gggagcctcg aggggatcct cgagggaatc ggacgaaacg
     2401 ggggagagga cggatgaaga aggaaacgga gagatcgtcg gcggcgggaa tggtggaagg
     2461 gttgacgttc gacgacgtgc tgctgatccc ggaggagtcc gccgtgatcc c
//
LOCUS       DASVJL010000447         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1579769, whole genome shotgun sequence.
ACCESSION   DASVJL010000447 DASVJL010000000
VERSION     DASVJL010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579769"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VF357_09410"
     CDS             complement(<1..459)
                     /locus_tag="VF357_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524408.1"
                     /translation="MSGKRIGAFICHCGGNISDYVDEEKVRAAAEKESGVAVAKTFLF
                     SCSEAAQQEMIRTIQEEKLDGIVVASCSPKLHLTTFRAMAQRSGLNPYMYTQVNVREQ
                     CSWAHRHEREEATGKAIHLVRAGIARTRLGVPLTDLRIETTPAALVVGAGV"
     gene            complement(456..>1626)
                     /locus_tag="VF357_09415"
     CDS             complement(456..>1626)
                     /locus_tag="VF357_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524409.1"
                     /translation="TSLPNVFAGGDDVTGPSMIIEAIAQGKRAAFYIDRSQQGLPLSG
                     ITFDARLSSVDGKEVLGRAEVPPSSRVPVVPLELPAAGRSRSMEEVEKTMTEEEARYS
                     AGRCLDCGICSECHQCVAACPAGAIHFEMREERETVEAGSVLVATGFELFDPKRKPEY
                     GFGEYPNVITAMQMDRLLAPTRPYNAVVRPADGMAPSNIAFVLCTGSRDCTVDNRLCS
                     RVCCMYSIKQAQLLMGALPLADVTIYYIDIRAFGKGYEEFYEQAKGMGVRFVKGKVAK
                     IDETGEGNLVVRYEDIDGSGGTLTAQHDLVVLSVGLSANPDAFGLFPPGSLEADPFRY
                     VREVDEDLEPGKTSIDGVFVAGTASAIRDIPDTILHSGAAATQVASFLQRKRAVL"
BASE COUNT          254 a          557 c          541 g          274 t
ORIGIN      
        1 gacgccggcc cccacgacca gcgccgcggg ggtcgtctcg atccggagat cggtcagcgg
       61 cacgccgagc cgggttcgcg cgatcccggc ccgcacgagg tggatcgcct tcccggtggc
      121 ctcttcccgc tcgtgccggt gcgcccagga acattgctcc cggacgttga cctgggtgta
      181 catatacggg ttcagcccgg accgctgcgc catggcgcgg aaggtggtga gatgcagttt
      241 cggggagcag gaggcgacca cgatgccgtc gagcttctcc tcctggatgg tccggatcat
      301 ctcctgctgg gcggcctccg aacaggagaa gaggaacgtc ttggccacgg cgaccccgga
      361 ttccttctcc gccgcggcgc ggaccttctc ctcgtcgacg tagtcggaga tgttcccgcc
      421 gcagtggcag atgaacgccc cgatccgttt tccgctcata gcaccgcccg ctttctctgg
      481 aggaaggacg cgacctgggt ggccgccgcc ccggagtgaa ggatcgtgtc ggggatgtcg
      541 cggatcgcgg aggccgtccc tgcgacgaac acgccgtcga tgctggtctt ccccggctcc
      601 agatcctcgt cgacctcccg gacgtaccgg aacgggtccg cttccaggga ccccggcggg
      661 aacaggccga aggcgtcggg gttggcggag aggcccaccg acaggacgac gaggtcatgc
      721 tgcgccgtca gcgtcccccc gcttccgtcg atgtcctcgt accgcacgac gaggttcccc
      781 tcgcccgtct cgtcgatctt cgccaccttc cccttcacga agcgcacgcc catccccttc
      841 gcctgctcgt agaactcctc gtaccctttc ccgaaggccc ggatgtcgat gtagtaaatg
      901 gtgacgtcgg ccagcggcaa cgcccccatg aggagctggg cctgcttgat cgagtacatg
      961 cagcagaccc gggagcagag ccggttgtcg acggtgcagt ctcgggagcc ggtgcacagg
     1021 acgaaggcga tattggacgg cgccatcccg tcggcggggc gcacgacggc gttgtacggt
     1081 cgggtggggg cgaggagccg atccatctgc atcgccgtga tcacgttcgg atactctccg
     1141 aaaccgtatt cgggcttcct cttcgggtcg aagagctcaa agcccgtggc gacgaggacg
     1201 gagccggcct cgacggtttc ccgttcctcc cgcatctcga agtggatggc gcctgcggga
     1261 caggccgcca cgcactggtg gcactccgag cagatcccgc agtccaggca gcggccggcg
     1321 ctgtaacggg cctcttcttc agtcatcgtt ttttccactt cctccatcga ccgggaacgg
     1381 ccggcggcgg gaagctccag cggaacgacc ggaacccgtg acgacggcgg cacctccgcc
     1441 cggccgagca cctccttgcc gtccaccgag gagaggcggg cgtcgaaggt gatcccggac
     1501 agcgggagcc cctgctgcga ccggtcgatg taaaaggcgg cgcgcttccc ctgcgcgatc
     1561 gcctcgatga tcatggaggg accggtcacg tcgtcgccgc cggcgaatac gttcggcagg
     1621 gaggtc
//
LOCUS       DASVJL010000448         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_77048, whole genome shotgun sequence.
ACCESSION   DASVJL010000448 DASVJL010000000
VERSION     DASVJL010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77048"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VF357_09420"
     CDS             <1..946
                     /locus_tag="VF357_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7524410.1"
                     /translation="GVITCVTGVSGSGKSTLILDTLYKALAQKLTGGRERPGEHRSLS
                     GLEQVDKVIHIDQSPIGRTPRSNPATYSGVFTPIRDLFAMLPESKSRGYRPGRYSFNV
                     KGGRCETCEGDGIIKIEMHFMPDVYVTCEECRGRRYNRETLEVAYKGKSVADVLEMTV
                     DQALAFLSPIPPIRHKLETLARVGLGYIRLGQSATTLSGGEAQRVKLARELSRRATGK
                     TVYLLDEPTTGLHFDDISKLLSVLHLFADAGNTVIIIEHNLDVIKSADHIIDLGPEGG
                     DAGGEIVACGTPEEVAGNPRSFTGHFLRRVLHLPAAAS"
     gene            complement(1253..1798)
                     /locus_tag="VF357_09425"
     CDS             complement(1253..1798)
                     /locus_tag="VF357_09425"
                     /inference="COORDINATES: protein motif:HMM:NF022751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HEX7524411.1"
                     /translation="MKIDPHVFQAKFPHHCVLDRCKSRCCRHGVWADIEEREAILRHA
                     DLFLPYVRAEAKNPTLWFGETAEDSDCPSGMAVETNVAGDACVFFHPAHGCSLQKAAI
                     DRGRHEWEFKPRFCIMFPLVVSEGVLTVDEDMDEVWCLKNENRTHPILDAVEKEVDHL
                     FPEEIARKLHKGNGAKKPSAA"
     gene            complement(2043..2375)
                     /locus_tag="VF357_09430"
     CDS             complement(2043..2375)
                     /locus_tag="VF357_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524412.1"
                     /translation="MFRRFTFSFLVALLFVAVASVAMAAEGTPAAGGDSNVKAVIALA
                     AGFGIAIAAFGGAMGQGKAIAAGLEGIARNPSAQNKIFIPMIVGLALIESLVIYALVI
                     AFVLVGKL"
BASE COUNT          465 a          839 c          861 g          431 t
ORIGIN      
        1 cggggtcatc acgtgcgtca ccggcgtctc gggctccggg aagtcgacgc tgatcctcga
       61 caccctctac aaggcgctcg cccagaagct caccggcgga cgggagcggc ccggggagca
      121 ccggtcgctg tcggggctgg aacaggtgga caaggtgatc catatcgacc agtcccccat
      181 cgggcgcacg ccgcgatcga acccggcgac ctactccggc gtcttcacgc cgatccggga
      241 cctgttcgcg atgctccccg aaagcaaatc ccgcgggtac cgtcccgggc gctactcctt
      301 caacgtgaag ggagggcggt gcgagacgtg cgagggggac gggatcataa agatcgagat
      361 gcacttcatg ccggatgtct acgtcacgtg cgaggagtgc cgggggcggc ggtacaaccg
      421 ggagacgctc gaggtcgcct acaaggggaa gagcgtcgcc gacgtcctcg agatgacggt
      481 ggaccaggcg ctggcgttcc tttcccccat cccgccgatc cggcacaagc tggaaacgct
      541 ggcgcgcgtg gggctggggt acatccggct ggggcagtcg gccaccacgc tgtcgggcgg
      601 ggaggcgcag cgggtgaagc tcgcgcggga gctgtcccgc cgggcgacgg ggaagacggt
      661 ctacctgctc gacgagccga cgaccggact ccacttcgac gacatctcga agctgctctc
      721 cgtcctccac ctcttcgcgg acgcggggaa cacggtgatc atcatcgagc acaacctgga
      781 cgtgatcaag tcggcggacc acatcatcga tctggggccg gaagggggcg acgccggcgg
      841 agagatcgtc gcgtgcggca cccccgagga ggtggcgggc aacccgcgct ccttcaccgg
      901 gcacttcctg cgccgggttc tccaccttcc cgccgcggcg agttgacgaa ccgcgcgcac
      961 caagcgctcc aattcataaa tacggcactg tgaaccgagg cgtttttatc tccgcgggat
     1021 acccccttcc tcgctacgtt ccggcagccg tccctggctg ccttgccgct cccgccatcc
     1081 atggctcccg cggcacttgc ccgtccatgg gcatcggggt tccacgaagc ggcgtctgga
     1141 gcgaagtgga gacagggacg tcgggaacga tcggagccgt agcggagtgg aggccccccg
     1201 cgagaccgga gttccctatg ccggattgat gccgtattta tgagttgaag ctctacgccg
     1261 cggacggttt cttcgcgccg ttccccttgt ggagtttgcg tgcgatttcc tcggggaaga
     1321 ggtggtccac ttccttctcc accgcgtcga ggatcgggtg ggtccggttc tcgttcttca
     1381 ggcaccacac ctcgtccatg tcctcgtcga cggtcagcac cccttccgag accacgagcg
     1441 ggaacatgat gcagaagcgc ggcttgaact cccactcgtg gcggccgcgg tcgatcgccg
     1501 ctttctgcag ggagcagccg tgggccggat ggaagaagac gcacgcgtcg ccggccacgt
     1561 tcgtctcgac cgccatgccg ctcgggcagt cggaatcttc cgccgtctcc ccgaaccaga
     1621 gggtcgggtt cttcgcctcc gcccggacat agggaaggaa gaggtccgcg tgccgcagga
     1681 tcgcctcccg ttcctcgatg tcggcccaga cgccgtgccg gcagcagcgg ctcttgcacc
     1741 ggtcgagcac gcagtggtgg gggaacttcg cttggaacac gtgcgggtcg atcttcatcg
     1801 gggaacacct ttccagcgtt cgtagaagtt gcccgcgcga acgacggacg aatatcttat
     1861 tctattcgcg cgaacgtccg cgggcaaggc cgacgagata ttttcctcct tcgagcgtga
     1921 agaaaatcgc ggcacttgcg gcggcccaaa gcgcaagggt acccgggaga agggggacgg
     1981 gaatgaaaaa gggctcccct aggagccctt tttcgcaaga cccgctttcg ccgtgcgccg
     2041 gcttagagct ttccgacgag gacgaacgcg atgaccagcg cgtagatgac gagggactcg
     2101 atcagcgcga ggccgacgat catcgggatg aagatcttgt tctgggcgga tgggttgcgc
     2161 gcgatcccct ccagcccggc tgcgatggct ttgccctggc ccatcgcgcc gccgaacgct
     2221 gcgatcgcga tgccgaaccc tgccgccaga gcgatgacgg ccttcacgtt ggaatcgccg
     2281 cccgcggcgg gagtgccttc cgccgccata gccaccgacg ccactgccac gaagagcaga
     2341 gcgacgagaa aagagaaagt gaacctgcgg aacattgggt gttccccctt tcaggatgtt
     2401 cccgggagcc ggttccgccc ggcggccggg gtagagccct tatcatcgac cgggggggtc
     2461 acccccggat caatgctcct ccgtgtgcgt cacggccccc gagatgtaga tcatcgagag
     2521 cacggtgaag atgaacgcct gcatgaacga aacgaagacg ccgaggccca tgaagaccga
     2581 ggggacgacg atgggg
//
LOCUS       DASVJL010000449         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_923603, whole genome shotgun sequence.
ACCESSION   DASVJL010000449 DASVJL010000000
VERSION     DASVJL010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923603"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1582)
                     /locus_tag="VF357_09435"
     CDS             complement(<1..1582)
                     /locus_tag="VF357_09435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX7524413.1"
                     /translation="MTLRVIPMGGLGEIGLNCMLFDDGGSALLIDAGLLFPDDTMLGV
                     DYVVPDFSVLRETAPHLGALLLTHGHEDHIGAVPFLLREFDIPVYGTRLTLGLLRHRL
                     AEQGLEASARLFPIARDARFRFGNFDVEAFPVCHSIPDAVGYVLRCPEGIFVHTGDFK
                     IDPFPLDGVPTGVERLRAISAAEGVTALFSDSTNVEREGVSPPEKFVGEALAEIFGQA
                     TGRVVVGMFSSNLHRIQEAIKAAGRCGRRVALCGRSMVRNVATAIDLGYLALPSPDVL
                     IPVEEAVSLPDRAVAVLTTGSQGEPRSALTLMALGEHKHLKVRPRDTIVLSSKFIPGN
                     ERAIAGLINRFFLAGADVLYEKISEIHVSGHASVEELRTMITAVRPANFVPVHGEPRL
                     LVRHRNLARDSGVPRVAMLRNGDVLAFSGGEMSLSGRIEVGRLFVDGKGIGDVESLVL
                     KDRVHLSQVGLVMVVLAFSSTTGELLYGPDIVTRGVVTESGSEALVEGARDEVLRAIE
                     EIGVDARTDTAEMQTAIRR"
     gene            complement(1579..2274)
                     /locus_tag="VF357_09440"
     CDS             complement(1579..2274)
                     /locus_tag="VF357_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7524414.1"
                     /translation="MRGALRSLVLVLLTAGSSVAAFLADRLLPGGNRAERVMGWWGRA
                     FIRSGGWKLRVEGMENLPSGGAVLVANHQSIVDIPMLLSAFPRPVGFLAKRELGEIPL
                     FGKAMAAAGNLFVDRDDPRDAVRMLREAGMRLRDGRLVVVFPEGTRSGDGSIGEFRPG
                     AFYLAQKSGAPVVPVYLDGGYRAIPKGGFRVRPAGLLVRVLPPLSPGEAAGGSKERIA
                     DAVRARLMREGER"
     gene            complement(2274..2852)
                     /locus_tag="VF357_09445"
     CDS             complement(2274..2852)
                     /locus_tag="VF357_09445"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEX7524415.1"
                     /translation="MPGVTGRDARPLEGMEETELFRRAFPDDGEGMRLAARGGEPADA
                     GRLLRWEAVLELARRTLAVPRRRPEPFRSAADVFERYRFLLSDSPVEVFVVVLLDVKH
                     RPLREERVSAGILDGSLIHPREVFSAAVRERSAAVLLLHNHPSGDPSPSGQDREVTRR
                     LRSAGGILGIPVVDHVILGDAAFFSFREEGDW"
     gene            2944..>3165
                     /locus_tag="VF357_09450"
     CDS             2944..>3165
                     /locus_tag="VF357_09450"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX7524416.1"
                     /translation="MEGIPLTELLSSFRENVNTVLLGKRESVDLAIASFIAGGHVLLE
                     DVPGTGKTTLARALSSGISGTFRRIQFTSD"
BASE COUNT          538 a         1149 c         1016 g          462 t
ORIGIN      
        1 cccgccggat cgcggtctgc atctccgccg tgtcggtccg cgcgtcgacc ccgatctcct
       61 cgatcgcgcg gagcacctcg tcccgcgccc cctcgacgag cgcctcggaa ccgctttcgg
      121 tcacgacgcc gcgtgtgacg atatcgggac cgtagaggag ttcgcccgtg gtcgaggaga
      181 aggcgagaac gaccatcacc agcccgactt gggagaggtg gacccgatcc ttgagaacga
      241 ggctctcgac gtcgccgatc cccttgccgt cgacgaagag gcgcccgacc tcgatccgcc
      301 cggagaggga catctccccc cccgagaacg cgagcacgtc gccgttccgc agcatcgcga
      361 cgcgcggcac ccccgagtcc cgcgccaggt tccggtgccg gacgagcagc cgcggctccc
      421 cgtggacggg gacgaaattc gccggacgga ccgcagtaat catcgtccgg agctcctcga
      481 cgcttgcatg gccggacacg tggatttccg atatcttctc atagagaaca tccgcccccg
      541 cgagaaaaaa acggttgatg aggcccgcga tcgcccgctc gttgccgggg atgaacttgg
      601 aggagaggac gatcgtgtcc ctcggccgca ccttcaggtg cttgtgctct ccgagcgcca
      661 tcaacgtcag cgccgaccgg ggctccccct ggcttccggt ggtgagcacc gccactgcgc
      721 ggtccggcag cgaaaccgcc tcctcgacgg ggatcaggac gtccggcgac ggaagcgcga
      781 ggtaaccgag gtcgatcgcc gtcgccacgt tccgcaccat gctccttccg caaagggcca
      841 cccgccggcc gcagcgtcca gccgccttga tcgcctcctg gatccgatgg aggttcgagg
      901 agaacatgcc caccacgacc ctccccgtgg cctgtccgaa gatctccgcg agggcctctc
      961 cgacgaactt ttccggcggg ctcaccccct cccgctccac gttcgtggaa tccgagaaga
     1021 gcgcggtgac cccttccgcg gcggagatcg cccgaaggcg ttccaccccg gtcggcacgc
     1081 cgtcgagcgg aaacgggtcg atcttgaaat cccccgtgtg gacgaagatc ccctcggggc
     1141 agcgcagcac gtaccccacg gcatcgggga tgctgtgaca gacggggaac gcttccacgt
     1201 cgaagttccc gaagcggaac cgggcgtccc gcgcgatcgg aaaaagtctc gcggacgctt
     1261 ccagcccctg ctcggcgagg cggtggcgca gcaggcccag cgtcagccgg gtgccgtaga
     1321 ccgggatgtc gaattcacgg agaaggaagg ggacggcgcc gatgtggtcc tcgtgcccgt
     1381 gggtgagcag gagcgcgccg aggtgggggg ccgtttcgcg cagaacggaa aagtcgggga
     1441 cgacgtagtc gacccccagc atcgtgtcgt cggggaacag gaggccggcg tcgatgagca
     1501 gcgcggagcc gccgtcgtcg aacagcatgc agttgaggcc gatctctccc agccccccca
     1561 tcgggatcac tcgcagcgtc atctctctcc ttccctcatt aatcgtgccc gcacggcatc
     1621 ggcgatccgc tctttcgatc cccccgccgc ttcacccggt gaaagcgggg gaagcacgcg
     1681 aacgaggagc ccggcgggcc gcacgcgaaa cccccccttg gggatcgccc ggtacccgcc
     1741 gtcgaggtag accgggacga cgggcgctcc ggatttctgc gcgaggtaaa aggccccggg
     1801 ccggaactcc ccgatcgacc cgtccccgct gcgggtccct tccgggaaga cgaccacgag
     1861 ccgtccgtcg cggagccgca tccccgcctc ccggagcatg cggaccgcat ccctcgggtc
     1921 gtcccggtcg acgaagagat tccccgccgc cgccattgcc ttcccgaaca gggggatctc
     1981 tcccagttcc cgtttcgcga gaaacccgac cggccgcggg aacgcggaca ggagcatcgg
     2041 gatgtccacg atactctgat ggttcgccac caggacggcc ccgccggacg ggaggttctc
     2101 catcccctcg acgcgcagct tccacccgcc cgaacgaatg aaggcgcggc cccaccatcc
     2161 catgacgcgt tccgcgcggt tcccgccggg aagcagccgg tcggccagga acgccgcgac
     2221 ggaggacccc gcggtgagga ggacgaggac cagggaccgc agcgcgcccc gcattaccaa
     2281 tctccctctt cccggaagct gaagaacgcc gcgtccccga gaataacatg gtcgaccacc
     2341 gggatcccga ggatcccccc cgcggaccgg agcctccgcg tcacctcccg gtcctgcccg
     2401 ctgggggagg gatcgccgct cgggtggttg tgcaggagga ggaccgccgc ggagcgttcc
     2461 cggaccgccg cggagaaaac ctctctcggg tggatgaggc tgccgtcgag gattcccgcc
     2521 gacacgcgct cctcccgcag gggtcggtgc ttcacgtcga ggagcaccac gacgaacacc
     2581 tccacgggcg aatcggagag caggaaccgg tatcgctcga acacgtccgc cgcggaacgg
     2641 aagggctccg gtcgtcgccg cggaaccgcg agcgtcctgc gcgcgagctc gagaaccgcc
     2701 tcccaccgga gaagcctccc cgcgtccgcc ggttcacctc cccgggcggc gaggcgcatc
     2761 ccttccccgt cgtcggggaa cgccctccgg aacagttccg tctcttccat gccttccagg
     2821 ggccgcgcgt cgcggccggt gacgcccggc attccgcctc ccgaggggaa atgccgggat
     2881 tccattcaaa taccattccg gatttttgtc aaagcgccgt ccggttgcga gataatgttt
     2941 cccgtggaag gaataccgct caccgagctg ctgtcgtcgt tccgcgagaa cgtaaatacc
     3001 gtcctcctcg ggaaacggga gtcagtcgac ctggcgatcg cctccttcat cgcgggaggc
     3061 cacgttctcc tcgaggacgt cccgggcacc ggcaagacga cgctggcgcg ggccctctcc
     3121 tcggggatct cggggacctt ccgccggatc cagttcacga gcgac
//
LOCUS       DASVJL010000450         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1205581, whole genome shotgun sequence.
ACCESSION   DASVJL010000450 DASVJL010000000
VERSION     DASVJL010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205581"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VF357_09455"
     CDS             <1..330
                     /locus_tag="VF357_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524417.1"
                     /translation="GEWGEPADDVTALTGNYLFASLQATGRLEGPFETLFRRFWDRYI
                     ERTTDAEILETAAPFFAFRCLVMASPVWFPSLDDSVRRKLFSFVLSILDTPRFEPEKV
                     NGWLDAL"
     gene            320..898
                     /gene="cysC"
                     /locus_tag="VF357_09460"
     CDS             320..898
                     /gene="cysC"
                     /locus_tag="VF357_09460"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX7524418.1"
                     /translation="MPSDRESAFAVWLTGLPASGKSTVAQALAAELGGKGIRAAVIES
                     DAVRREITPNAGYGEAERNAFYATVAYLARMLVLHGVPVIVDATANRRAYRDRARAVI
                     PRFLEAYVRCPLAVCQARDPKGIYRRGAAGTAQNVPGVSTPYEPPLTPEVVVDGERDD
                     PAVAARHIVSALEKKGYLPPGPGDATKTGKRL"
     gene            complement(893..1435)
                     /locus_tag="VF357_09465"
     CDS             complement(893..1435)
                     /locus_tag="VF357_09465"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX7524419.1"
                     /translation="MEKNVSGKVIELVLGDITRQRVDAIVNAANSTLLGGGGVDGAIH
                     RAGGPLILEECRAIRSARGECPTGEAVLTGGGDLPARYVIHTVGPVWRGGNQGEPGLL
                     ASCYRTALRIAAEQGFRTVSFPSISTGIYGYPVSLAAPTAIAAVASFLSSEPVTPSRV
                     RFVLHDPVTFATYAGALEAL"
     gene            1470..>1984
                     /locus_tag="VF357_09470"
     CDS             1470..>1984
                     /locus_tag="VF357_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524420.1"
                     /translation="MRQALHRTLRRRPPLRRLLRDLARAIQSAGGTVYLVGGFLRNIV
                     EGREPGDVDLLVTGLSYRRTGDVLQSLSVMVPGIRKVVAAGKHFPVHRIAVDWGKEYI
                     DISNARAGGSPRPLRPGAARDDVHGWPSAGTGRDAPGSVVPADDREAVDDAARRDFTI
                     NGLLFRLAPGRT"
BASE COUNT          312 a          652 c          708 g          312 t
ORIGIN      
        1 ggcgagtggg gggaaccggc ggacgacgtg accgccctca cggggaacta cctgttcgcg
       61 tcgctgcagg cgacgggccg cctcgaaggc cccttcgaaa cgttgttccg gagattctgg
      121 gaccggtaca tcgagcggac gacggacgcg gagatcctcg agaccgccgc gccgttcttc
      181 gcgttccgct gcctcgtgat ggcctccccc gtctggttcc cgtccctcga cgattcggtt
      241 cgccggaagc tcttctcctt cgtcctctcg atcctcgaca caccgcgctt cgagccggag
      301 aaggtcaacg gttggctcga tgccctctga tcgcgaatcc gctttcgcgg tctggctcac
      361 ggggctaccc gcttcgggaa agtcgaccgt cgcgcaggcg ctcgcggcgg aactgggagg
      421 gaaaggaatc cgggcggcgg tcatcgagtc cgacgccgtt cgccgggaga tcactccgaa
      481 tgccggctac ggagaggcgg agcggaatgc gttctacgcg accgttgcgt acctcgcccg
      541 gatgctcgtc ctgcacgggg tccccgtgat cgtcgacgcg acggcgaacc gcagggcata
      601 ccgtgaccgc gcccgggcgg tgatcccccg gttcctcgag gcgtacgtcc gctgcccgct
      661 cgcggtgtgc caggcgcggg acccgaaggg gatctaccgg cgcggggcgg cagggacggc
      721 gcaaaacgtg ccgggcgtgt cgacgccgta cgagccgccg ttgacaccgg aggtggtcgt
      781 cgacggagag agggacgatc ccgcggtcgc cgccaggcac atcgtgtcgg cgctggagaa
      841 gaagggatac cttccgcccg gtccgggaga cgcgacgaaa acggggaaac ggttataacg
      901 cttcgagcgc ccccgcgtag gtggcgaacg tcacggggtc gtggaggacg aagcggacgc
      961 gggagggagt gacgggttcg gaggagagga acgatgcgac cgccgcgatc gccgtcgggg
     1021 ctgccagcga aaccggatac ccatagatcc cggtgctgat ggaggggaag gacacggtcc
     1081 ggaaaccttg ctccgcggcg atgcggaggg cggtccggta gcaggaggcg agcaggccgg
     1141 gctctccctg gttgccgccg cgccataccg gtccgacggt gtggatcacg taccgcgccg
     1201 gcaaatcgcc gccgccggtc aggaccgcct caccggtggg gcattctccg cgcgcggacc
     1261 ggatcgcgcg gcactcctcg aggatcaacg ggccgccggc acggtggatc gcgccgtcga
     1321 cccctccgcc gccgaggagc gtggagttcg ccgcgttcac gatcgcatcg accctctggc
     1381 gcgtgatgtc gccgaggacg agttcgatca ctttcccgga gacgtttttt tccatggggc
     1441 acattatacg atcaaatcgg accgattgga tgcggcaggc gttgcaccgc actctccgcc
     1501 gccgacctcc cttgcgccga ctactccgcg acctcgcccg ggcgatccag agtgccggag
     1561 ggacggtata tctcgtcggg gggttcctgc ggaacattgt cgagggaagg gaaccggggg
     1621 acgtagacct gctggtcacg gggctttcgt accggcggac cggggatgtt ctccagtcgc
     1681 tttccgtcat ggtgccggga atccggaaag tcgtggcggc gggcaagcat ttcccggtgc
     1741 accggatcgc ggtcgattgg gggaaggagt acatcgacat ctccaacgcg cgcgccggcg
     1801 gctcccctcg cccgctgcgg cccggagccg cacgcgacga cgtccatgga tggcctagtg
     1861 ccgggacagg cagggatgcc ccgggttctg tcgtcccggc cgacgatcgg gaagccgtcg
     1921 atgacgcggc ccggcgggac ttcaccatca acggtctcct cttccggctc gcccccggcc
     1981 gcac
//
LOCUS       DASVJL010000451         5917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1861912, whole genome shotgun sequence.
ACCESSION   DASVJL010000451 DASVJL010000000
VERSION     DASVJL010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861912"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..935)
                     /locus_tag="VF357_09475"
     CDS             complement(54..935)
                     /locus_tag="VF357_09475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7524421.1"
                     /translation="MEYDFPLFRPPSEARSLIFQVTLGCSWNRCLFCTSYRTKEFRVR
                     PFDEVQRDVVEVSGCYPDTRKIFLADGDPMAAPTDYLLKVLDLMNRRFPSLERISTYA
                     GPTNLMAKSSEELRMLEERKLDLLYLGIETGNDELLKRVRKGATARQIIEGGRKALQA
                     GLRMSAFIILGLGGVDGSYEHAKDSAHVVNGIDPQFLATLTLIPIPDKGVYEERIMGG
                     GFRIIDRKQSLQELRWFVEDLALTDCKFGTEHASNYLPIHGAMLPRDKSRILNLIDKA
                     LKETSPGMFRPEWARGL"
     gene            990..1463
                     /locus_tag="VF357_09480"
     CDS             990..1463
                     /locus_tag="VF357_09480"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX7524422.1"
                     /translation="MPADAKPVSATRLVMAQEMTPQDANPAGNVHGGNIMRLADSAAG
                     VVAIRHSGRNCVTAAVDHFEFHAPVFIGNLVTFHASVNHVGRTSMEVGVRVEAEEPRT
                     GKRTHTNSSYFLMVALDDEGRPVEVPKLLLETAEDRRRNEEAMQRAIARRQRKKK"
     gene            1478..1732
                     /locus_tag="VF357_09485"
     CDS             1478..1732
                     /locus_tag="VF357_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524423.1"
                     /translation="MTSDRRIRQGSRVLLGIALFAGTLMSMCGGALLADFALDLLGVA
                     SPPVRLGVKVIAMAAALPAGFYLVERAFLARSSASVASRK"
     gene            complement(2007..3071)
                     /locus_tag="VF357_09490"
     CDS             complement(2007..3071)
                     /locus_tag="VF357_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524424.1"
                     /translation="MSSSHSALQKYERALNRYFQTPAAARKTEDREKILKILGVENPQ
                     EFLGMHIPLWEAKLDELLDPTSTDMLPISIAHSYVNWVRGAIRMMPAEARVTIFSSKF
                     KATGLKKSILTLLHEMTGKPHRDFEVTEVLLIEKVHKDTLFTVRTPDGMERDIYLSRF
                     GCMGEYVYSGLPALVGLPALPTVYHVTPQGEEVLLKPKEEGTNIYLDDSVTLARINRD
                     GVWWVAGAAWQDALGDCIGTALRYGHYVATPKKEVVMIDNIELFHLEETDVRIFEPIY
                     EFLPKKAYPEDQPKRVRLQEKMQKEYEAAYAAQRTVIRKEWPEIERYLIEMRRNIHAY
                     AGEVFEMVMTRVKAQVFSGN"
     gene            complement(3131..5128)
                     /locus_tag="VF357_09495"
     CDS             complement(3131..5128)
                     /locus_tag="VF357_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7524425.1"
                     /translation="MTDLPLVLRDLSIVLAIATAVALLFGRLHLSVVAAFLVAGAILG
                     PTGAGLVAESGMVEPLAEIGVALLLFTVGMEISLANLGKMRRRILQGGGIQLSATILF
                     TVAVLSLAKFPLAEAIFIGFVLSLSSTAIVLKVYADRMEIDSGHGKISIGILLFQDMA
                     VIPMMLLIPSFRQWETAHFSTVAFTLAKAGAGVGVILLASRFMIPLLLKEVIRLNSRE
                     ILAMTVMCLILGTAWIARWWGLSLAMGAFLAGLVISESMYVHEIAAQIFPFRDVFNGV
                     FFISVGMLLDLSFLAHHLPVILLFSIGVVLAKGICAGAAIRTLDYPWRVSVIGAIGLA
                     QVGEFSFLLLSEGSRDGLVGAETYQYLLATAILTMVATPFLMRSAPWAGRVFVRHLIR
                     GPEPEDSGEESAGAARVENHVIISGYGMNGKNLARVLRSTHVPYIVVDLNDTLVREGR
                     EAGEPIFYGDVNNPEILDRVGVGRARMLVLAISDPMATRRAVAVARRANPRLGILVRT
                     RYVADVDDLIALGANAVIPEEFETSVEIFSRVLREYHVPDHVVSQQEELIRSGTYRIL
                     RERIPSRDEGLLSEFEVFLRQKVIEVFYVSRDSPWAGRPLGDLPAGNGAGIVLLAVLR
                     EDRAIVQPSPEEKIAVGDKLVFFGGHGPLAATLEELAKGSR"
     gene            complement(5416..>5917)
                     /locus_tag="VF357_09500"
     CDS             complement(5416..>5917)
                     /locus_tag="VF357_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7524426.1"
                     /translation="DGYTFIAAYNDEAFDSLMHIIGRPELARDPRFSTPKNRVALENE
                     RALLEILEEWSGDRTADEILGAVEEYTSKRGGPAAAVVTGRVNRPLETLSEGHWRERG
                     CFLRARDPVYGELLLAAPPWKMSGTPARWKSGCRPPGSDNRDVYLGILGMTEEEYRRL
                     AEAGTF"
BASE COUNT         1068 a         1933 c         1906 g         1010 t
ORIGIN      
        1 cgaacagaaa gaatcttcgg gtgtcgtatt tgagaaaatc catgttccct cctctagagc
       61 cccctcgccc actccggccg gaacatgccc ggggaggtct ccttcaaggc cttgtctatg
      121 aggttcagga tccggctttt gtccctgggc agcatcgccc catggatagg gaggtagttg
      181 gacgcgtgct ccgtgccgaa cttgcagtcg gtgagcgcaa gatcctccac gaaccaccgg
      241 agctcctgca gggactgctt cctgtcgatg atccggaacc ctcctcccat gatcctctcc
      301 tcgtagacac ccttgtctgg tattgggatc agtgtgagag tggccaggaa ctgggggtcg
      361 atgccattga ccacgtgggc ggaatctttc gcatgttcat agctcccatc caccccaccg
      421 agccccagga tgatgaaggc ggacatgcgc agtcccgcct gaagggcctt cctgccgccc
      481 tcgatgatct gtctggcggt tgcgcccttc ctgacccttt tcagcagttc gtcgttcccc
      541 gtctctatcc cgaggtagag gagatcgagt ttgcgctcct cgagcatcct cagctcttcc
      601 gacgactttg ccataaggtt cgtggggccg gcatacgtgc tgatccgttc gagggagggg
      661 aaccgccggt tcatgaggtc gagcaccttc agtaggtagt cggtcggcgc cgccatcggg
      721 tcgccgtcgg caaggaagat cttcctggtg tccgggtagc acccggacac ctccacgaca
      781 tccctctgca cctcatcgaa agggcggacc cggaattcct tggtcctgta cgaggtgcag
      841 aacaggcacc ggttccagga gcagccgagg gtcacctgaa aaatgaggga cctcgcctcc
      901 gatggcggcc tgaacagcgg gaagtcgtat tccatggtgt tatcaggtta ccatcttcaa
      961 aggtaccttc gcgtggagag gaggatggga tgcctgcgga tgcgaagccg gtatcggcga
     1021 cccggctggt gatggcgcag gagatgacgc cccaggacgc gaaccccgcg gggaacgtcc
     1081 acggggggaa tatcatgagg cttgccgact cggccgccgg cgtggtggcc atccggcact
     1141 ccggcaggaa ctgcgtcacc gccgccgtcg accacttcga attccacgcg cccgtcttca
     1201 tcggcaacct cgtcaccttt cacgcttccg tgaaccatgt ggggcgcacc tcgatggagg
     1261 tcggagtccg cgtcgaggcg gaggaaccgc gcacggggaa gaggacccac acgaattcgt
     1321 cctatttcct gatggtggcg ctggacgacg agggaaggcc ggtcgaggtt ccgaagctgc
     1381 tgctggagac cgcggaggac cgccgcagga atgaggaggc gatgcagcgg gcgatcgccc
     1441 ggcggcagcg aaagaaaaaa tagggacggg cgaaaggatg acgagcgatc ggcggatacg
     1501 acaaggatcg cgggtgctgc tggggatcgc gctcttcgcg ggaaccctca tgtcgatgtg
     1561 cgggggggcg cttctcgcgg atttcgcgtt ggacctgctg ggcgtcgcct cgccgccggt
     1621 ccgtctaggg gtgaaggtga tcgccatggc cgccgcgctg cccgcggggt tctatctcgt
     1681 cgaacgcgcc ttcctcgcgc ggtcctccgc gagtgtagcg tctcgcaaat aacctgacgc
     1741 atcgagagcg tcgatgcgcc gcgccagcaa ggcgcgcaag tgaagacgta tcgcgagagt
     1801 acggcgaact tgccgcccca ctcccggatg aaatacgctc cctggggtac cccggacagc
     1861 cgatgccatg taccttgtgg tcggcatgtg gggcacaacg aagctggagg cggcagcaac
     1921 ggccatggat ggccaagtgc cgcggagggc atggatgccc gggagcggcc ggcgctcgaa
     1981 tgccagggta tttgcgaaac gttgcactag ttcccggaaa acacctgggc cttgacgcgg
     2041 gtcatcacca tctcgaacac ctcgcccgcg taggcgtgga tgttgcggcg catctcgatc
     2101 aggtaccgct cgatctcggg ccactccttc cggatcaccg ttctctgcgc ggcataggcc
     2161 gcctcgtact ccttctgcat cttttcctga aggcggaccc gcttcggctg gtcctccgga
     2221 tacgccttct tcgggaggaa ctcgtagatc ggctcgaaga tgcggacgtc ggtctcctcc
     2281 aggtggaaca gctcgatgtt gtcgatcatc accacttcct tcttcggggt ggcgacgtag
     2341 tgcccgtagc gcagcgccgt tccgatgcag tcccccaggg cgtcctgcca ggccgcgccc
     2401 gccacccacc acacgccgtc gcggttgatc cgggcgaggg tcaccgaatc gtcgaggtag
     2461 atgttggtcc cctcctcctt cggcttgagg agcacctcct cgccctgcgg cgtcacgtgg
     2521 tagacggtcg ggagggcggg cagccccacc agcgccggca ggccgctgta gacgtactct
     2581 cccatgcacc cgaagcggga cagatagatg tcgcgttcca tcccgtccgg ggtccggacc
     2641 gtgaagagcg tgtccttgtg gaccttctcg atcagcagga cctcggtgac ctcgaagtcg
     2701 cggtgcggct tccccgtcat ctcgtggagc agcgtcagga tcgacttttt cagtccggtc
     2761 gccttgaact tggaggagaa aatcgtcacg cgcgcctcgg ccggcatcat ccggatcgcg
     2821 ccgcgcaccc agttgacgta ggagtgggcg atgctgatcg ggagcatgtc ggtgctcgtc
     2881 gggtcgagga gttcgtcaag cttcgcttcc caaagcggga tgtgcatgcc gaggaactcc
     2941 tgcgggttct cgacccccag gatcttcagg attttctccc tgtcctcggt cttccgcgcg
     3001 gcggcgggtg tctgaaaata ccggttcagg gcgcgctcgt acttctgcag agcggaatgg
     3061 gaagaagaca tcattgccct cccggccgat gaagtgggaa catcatgatc ttatcaccgg
     3121 cggcaccttg ttagcgtgat ccctttgcca gctcctcgag ggtagccgcc aggggaccat
     3181 gccccccgaa gaagacgagc ttgtccccca ccgcgatctt ctcctccggg gacggctgta
     3241 cgatcgcgcg gtcctcccga aggacggcga ggaggacgat cccggcgccg tttccggccg
     3301 gaagatcccc cagaggccgt cccgcccaag gcgagtcccg ggaaacatag aagacctcga
     3361 tcaccttctg ccggaggaac acctcgaact ccgacagcag cccctcgtcc ctggaaggga
     3421 tacgctcccg caggatccgg tacgtcccgc tgcggatcag ctcttcctgc tgggagacga
     3481 cgtggtcggg gacgtggtac tcgcgcagga cccgggagaa gatctccacc gacgtctcga
     3541 actcctcggg gatgacggcg ttcgccccca gggcgatcag gtcgtccacg tccgccacgt
     3601 accgcgtcct cacgagaatg cccagccgcg ggttggcccg ccgggccacc gccacggcgc
     3661 ggcgggtcgc catcgggtcg gagatggcca gcaccagcat ccgggcgcgg cccaccccga
     3721 cgcggtcgag gatctccggg ttgttcacgt caccgtagaa gatcggctcc cccgcctccc
     3781 ggccctcccg caccagcgtg tcgttcaggt cgacgacgat gtagggaacg tgcgtcgatc
     3841 gcagcacgcg ggcgaggttc tttccgttca tcccgtaccc ggagatgatc acgtggttct
     3901 cgacccgtgc ggcaccggcg gactcctccc cggagtcctc cggttcgggc ccccggataa
     3961 ggtggcggac gaagaccctc ccggcccatg gagccgaccg catcaggaac ggagtggcca
     4021 ccatcgtcag gatcgcggtt gcaagaagat actggtacgt ctcggcgccc accaggccat
     4081 cgcgggatcc ttcggacaac aggaggaacg agaactcgcc gacctgcgcc aacccgatcg
     4141 cgccgatcac ggagacccgc caggggtagt ccagggtccg gatcgcggcg ccggcgcaga
     4201 tccccttcgc cagaacgacc ccgatggaaa agaggaggat aacgggaagg tggtgcgcga
     4261 gaaacgaaag gtcgagcagc atcccgacgg agatgaaaaa caccccgttg aagacgtccc
     4321 ggaaggggaa gatctgggcg gcgatctcgt ggacatacat cgactcggaa atcaccagtc
     4381 cggcgaggaa cgcccccatc gccagggaga gcccccacca ccgggcgatc cacgccgtcc
     4441 cgaggatgag gcacatcacc gtcatcgcca ggatttcccg gctgttcagc cggatgactt
     4501 ccttgaggag gagcgggatc atgaaacgcg acgccagcag gatgaccccg acgcccgcgc
     4561 ccgccttggc cagcgtgaat gccacggtgg agaagtgcgc cgtctcccat tgccggaacg
     4621 aggggatgag cagcatcatc gggatcacgg ccatgtcctg gaacaggagg atgccgatgg
     4681 agatcttccc gtgaccgctg tcgatctcca tccggtcggc gtacaccttc aggacgatcg
     4741 cggtgctcga aagggacagg acgaacccga tgaagatcgc ttccgcgagg ggaaacttcg
     4801 cgagggaaag gacggccacg gtgaacagga tcgtcgccga gagctggatc ccaccgccct
     4861 gcaggatgcg tcggcgcatc ttccccaggt tcgcgaggga gatctccatc ccgacggtga
     4921 acagcaggag ggcgacgccg atctcggcga ggggttccac catcccggac tcggccacca
     4981 gccccgctcc ggtcgggccg aggatggcgc cggcgaccag gaacgccgcc acgacggaca
     5041 ggtggagccg cccgaacagc agcgcaaccg ccgtggcgat cgccaggacg atggacaggt
     5101 cgcgaaggac caggggcagg tcggtcaacg attctcccgg aggcgggttg cttagtgcag
     5161 cgtctcgcta cactagatca gtctaccagt ccggggacca tcaagtgaac cgatcccctc
     5221 gcggggcggg gaaaaatccc ctgaagttct cctgacggaa ttccgaaaag aggatacgga
     5281 agaacggcaa cgcctgcctc ccctccccgg ggaggcaacg gcccgcacac ccctccaacg
     5341 gttcgcgggc gacgcgaacg gtcccccggg gttcccgcct gcgccccggg ggattcgctt
     5401 tcccggtccg gccggtcaga aggtccccgc ctccgccagc cgtcggtact cctcttccgt
     5461 catcccgagg atcccgaggt acacgtcccg gttgtccgac cccgggggcc ggcaccccga
     5521 cttccaccgc gccggggtcc cgctcatctt ccaggggggc gcggcgagca gaagttcacc
     5581 gtagacgggg tcccgcgcgc gcagaaaaca ccctctctcc cgccaatgcc cttcggaaag
     5641 cgtctctaga gggcggttca cccgccccgt gaccacggcg gcggcgggac cgccccgttt
     5701 cgaggtgtac tcctcgaccg ccccgaggat ctcgtcggcc gtgcgatcgc cggaccactc
     5761 ctcgaggatc tccaggagcg cccgttcgtt ctcgagggcg acgcggttct tcggcgtgga
     5821 gaaccgcggg tcgcgggcca gttccggtcg gccgatgatg tgcatcagcg aatcgaacgc
     5881 ctcgtcgttg taggcggcga tgaaggtgta gccgtcc
//
LOCUS       DASVJL010000452         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1110776, whole genome shotgun sequence.
ACCESSION   DASVJL010000452 DASVJL010000000
VERSION     DASVJL010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110776"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="VF357_09505"
     CDS             <1..1641
                     /locus_tag="VF357_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX7524427.1"
                     /translation="FGPDAMLSANASIYGVRDLPEHDLANTRYLLSFSGDFLETGLSP
                     VHYAREYGRMRGDRETIRGKFVHFGPRLSMTAAAADLFLPCTPGTEGIVALGIAHVMV
                     RDRLSAASDAAGQLADDLSAYSPEAVERRTGVHAHDIAAVAEEFARNQPGLAIAGTAA
                     ATGSEGAFHCAAVALLNVLAGNVGKPGGVSFPDRAQAFAKYGTEAALLAPTPESGYAG
                     IRAALDKMRGGAFRMAILSGATNPAFTLPPSLKFGEALSKVPFVVAFAPFLDETTSQA
                     DLVLPVPTALEAWGDDLAPVGHAGAVTLCQPVVEPFHDTRSMPDVLIAAASELGGEPA
                     RALPWASFRECIEKAYGGPGGEMEKALQRGGFFGVSSSPRSAARTGNIALPKSAERAI
                     DADPKAFPLALHLYPSITLYDGRGANLPWLQEMPDPVTTAVWRNWVEVNPKTAAGLGL
                     SDGDGVTVTSPSGKITAHVAFNPGIAPDVAAMPLGQGHTRYGKTANGRGENPFGLLPA
                     GQTYSLQATRVALTKTKLPIRLVRFAHPEGQWKISNLM"
     gene            1697..2395
                     /locus_tag="VF357_09510"
     CDS             1697..2395
                     /locus_tag="VF357_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7524428.1"
                     /translation="MSHKWAMAIDLDRCTGCGACVVACVAENNIATVGEAEVEKGRLM
                     QWLRVARYWEGEFPEAHAVYLPVPCMQCERAPCETVCPVYATYHNEDGLNAMVYNRCV
                     GTRYCANNCPYSVRAFNWFDPKWEKPLDEQLSPDLTTRSRGVMEKCTFCIQRIRRAKE
                     TAAQEGREIRDGEVLPACVQTCPPGALLFGDLADPHSEITRKLKDPRRFRLMEHLGTE
                     PRVVYLKRQKGAGR"
     gene            2392..>3771
                     /gene="nrfD"
                     /locus_tag="VF357_09515"
     CDS             2392..>3771
                     /gene="nrfD"
                     /locus_tag="VF357_09515"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX7524429.1"
                     /translation="MNGSDHLARTDPGALYRELSRSNLPTTKRWYALAGLLALVTLWG
                     AGCFVYMMKVGMGVTGLNRPVMWGALIASFVFWIGLSHSGTLISAILRLTNATWRAPI
                     LRGAEAMTAFALMVGGLFPLIHIGRNWRFYYMIPYPNSRHLWPNLRSPLMWDAVAINT
                     YLLGSLTFLYFGMIPDLAIARDHATGWRKKFYTVLALGFRGTHNEWRRYHVASTIFAI
                     LIIPVAVSVHSIVGWDFAMAKVPGWHSTIFAPYFVTGAIFSGIAGVVIVMATLRKVFR
                     LERVLTVVQFENLGKLLCTMSLLWGYFYFSDFLTVWYNRNPEEWEVFRSYAADYLPLF
                     LVMIGCNFVVSFPLLCMRRVRRSIPALSVVSLMIVIGMYAERFMIVVPSLARRNDPFI
                     WHNYFPTWVEFSILAGALAMFLLLYMIFAKLFPIMAISDIQEHLFHTTDRKIGDANIE
                     SIAMPHDTGV"
BASE COUNT          619 a         1299 c         1219 g          634 t
ORIGIN      
        1 ttcggccccg acgcgatgct gtccgcgaac gcgtcgatct acggcgtccg cgaccttccg
       61 gagcacgacc tcgccaacac gcgatacctg ctctccttct ccggcgactt tctcgagacg
      121 gggctctccc ccgtgcacta cgcaagggag tacgggcgga tgcgcggaga ccgtgagacg
      181 atccgcggga agttcgtcca cttcggcccc cggctctcga tgaccgcggc cgccgcggac
      241 ctgttccttc cgtgcacccc cggcacggaa ggaatcgtcg ccctcgggat cgcgcacgta
      301 atggtgcggg accgtctctc cgccgcatcc gacgccgcgg ggcaacttgc ggacgacctg
      361 tccgcatatt ccccggaggc ggtcgagaga agaacggggg tccacgccca cgatatcgcc
      421 gcggtggcgg aggagttcgc gcgcaaccag ccgggccttg cgatcgcggg aacggcggcc
      481 gcgacaggct ccgaaggggc gttccactgc gccgccgtgg cgctcctgaa cgtcctcgcc
      541 gggaacgtgg gaaagcccgg cggggtgtcg ttcccggacc gcgcgcaggc gttcgcgaag
      601 tacggtacgg aagccgccct gctcgcccca accccggaga gcgggtacgc ggggatacgc
      661 gccgcactcg ataaaatgcg cggtggcgcg ttccggatgg cgatcctgtc gggggcgacg
      721 aaccccgcgt tcaccctccc gccttcgctc aaattcggcg aagcgttgtc gaaggtcccc
      781 ttcgtcgtcg ccttcgcccc cttcctcgac gagacgacgt cgcaggccga cctggtgctt
      841 ccggttccga ccgcgctcga ggcgtggggg gacgatctcg cccccgtggg gcacgccggg
      901 gcggtcaccc tctgccagcc ggtggtggaa ccgttccacg acacccggtc gatgcccgac
      961 gtcctgatcg ccgcggcgag cgaactgggc ggggaaccgg caagagcgct gccgtgggct
     1021 tctttcaggg agtgcatcga gaaggcgtac ggcggtcccg gcggagagat ggagaaagcc
     1081 ctccagcgcg gcggcttctt cggggtgtct tcctctccgc ggtccgcggc ccgaacgggg
     1141 aacatcgccc tcccgaaatc ggcggagcgc gccatcgacg ccgacccgaa agcgttcccc
     1201 ttggcgctgc acctctaccc ctccatcact ctctacgacg ggcgcggggc gaacctcccg
     1261 tggctccagg agatgcccga cccggtaacg accgcggtgt ggcggaactg ggtcgaggtg
     1321 aacccgaaga cggccgcggg gctgggcctc tccgatgggg acggcgtgac cgtcacgtcg
     1381 ccttcaggga agatcacggc ccacgtggcg ttcaacccgg ggatcgcgcc ggacgtcgcg
     1441 gcgatgcccc tgggacaggg acacacgcgc tacgggaaaa ccgcgaacgg aagaggggag
     1501 aacccgttcg gcctcctgcc ggcggggcag acatactcgc tccaggcaac acgggtcgcc
     1561 ctgacgaaga cgaaacttcc catccggctg gtccggttcg cgcacccgga ggggcaatgg
     1621 aagatcagca acctgatgtg atcgacggga aatctcagga acgtccccat tctgcgaatg
     1681 agggtcggga gaagcaatga gccacaagtg ggcgatggcg atcgacctgg accggtgcac
     1741 cggatgcgga gcgtgcgtcg tcgcgtgcgt cgcggagaac aacatcgcca ccgtcggcga
     1801 ggccgaggtg gaaaaagggc gcctgatgca atggctccgg gtcgcccgtt actgggaggg
     1861 ggaattcccg gaggcgcatg cggtttatct ccccgtgccg tgcatgcagt gcgaacgcgc
     1921 cccgtgcgag accgtctgcc cggtctacgc gacctaccac aacgaggacg ggctgaacgc
     1981 gatggtctat aaccgatgcg tcgggacccg ctactgcgcg aacaactgcc cctactcggt
     2041 gcgcgccttc aactggttcg atccaaagtg ggaaaaaccc ctcgacgagc agctctcccc
     2101 cgacctcacc acccgatccc gcggcgtcat ggaaaagtgc accttctgca tccagcggat
     2161 tcggcgcgcg aaggagaccg cggcgcagga aggacgggag atccgcgacg gggaggtcct
     2221 tcccgcgtgt gtccagacgt gcccgccggg agcgctcctc ttcggggacc tggccgatcc
     2281 gcacagcgag atcacccgga agctgaagga cccccgccgg ttccgcctga tggagcacct
     2341 gggcaccgag ccgcgggtgg tgtacctgaa gcgccagaaa ggggccggtc gatgaacggg
     2401 agcgaccatc tggcccggac cgatccgggc gcgctttatc gggagctgtc ccggtcgaac
     2461 ctccccacga cgaagcgatg gtacgcgctc gcgggcctcc ttgcgctggt aaccctttgg
     2521 ggagcgggtt gcttcgtcta catgatgaag gtcgggatgg gagtcacggg gctcaaccgg
     2581 ccggtcatgt ggggggccct gatcgcatcg ttcgtcttct ggatcggcct gtcccactcg
     2641 ggcaccctca tctcggccat cctccgcctc accaacgcca cctggcgcgc ccccatcctg
     2701 cgcggggcgg aggcgatgac cgccttcgcg ctgatggtcg gcggcctctt cccgctgatc
     2761 cacatcgggc ggaactggcg gttctactac atgatcccct accccaactc gcggcacctg
     2821 tggcccaacc tccgatcccc cctcatgtgg gacgcggtgg cgatcaacac gtacctgctc
     2881 gggagcctca ccttcctcta cttcgggatg atccccgacc tggcgatcgc ccgcgaccac
     2941 gcgacagggt ggcggaaaaa gttctacacc gtgctcgcgc tggggttccg cgggacgcac
     3001 aacgagtggc ggcggtacca cgtcgcctcg acgatcttcg cgatcctcat catcccggtg
     3061 gccgtctcgg tccactcgat cgtcggatgg gacttcgcga tggcgaaggt ccccgggtgg
     3121 cacagcacga tcttcgcccc ctacttcgtc acgggcgcga tcttctccgg gatcgccggc
     3181 gtggtgatcg tcatggccac gctgcgaaag gtgttccgcc tggagcgggt gctcacggtg
     3241 gtgcagttcg agaacctcgg aaaactgctg tgcaccatgt cgctcttgtg gggctatttc
     3301 tacttctcgg acttcctcac cgtctggtac aaccggaacc ccgaggaatg ggaggtcttc
     3361 cggtcgtacg ccgccgacta cctccccctg ttcctcgtca tgatcgggtg caacttcgtc
     3421 gtctccttcc cgcttctgtg catgcgcagg gtccgccggt cgatccccgc cctcagcgtc
     3481 gtctccctca tgatcgtcat cgggatgtac gcggagcgct tcatgatcgt cgtcccgtcg
     3541 ctggcgcgtc gcaacgaccc gttcatctgg cacaactact tccccacctg ggtggagttc
     3601 tcgattctcg ccggggcgct cgccatgttc ctcctgctgt acatgatctt cgccaagctc
     3661 ttcccgatca tggcgatcag cgacatccag gagcatctct tccacaccac cgaccggaag
     3721 atcggcgacg cgaacatcga gtcgatcgca atgccgcacg acaccggggt a
//
LOCUS       DASVJL010000453         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1672705, whole genome shotgun sequence.
ACCESSION   DASVJL010000453 DASVJL010000000
VERSION     DASVJL010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672705"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VF357_09520"
     CDS             <1..298
                     /locus_tag="VF357_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524430.1"
                     /translation="RPDGRFTLLLKHRPQLDPATGGKFDLQLSGHTHDGQVFPFRLLT
                     RLAFPLLGGDHPVPGGGILHVSRGTGTWGPPMRFLAPPEVTIVEIERRTPSGPS"
     gene            295..1473
                     /locus_tag="VF357_09525"
     CDS             295..1473
                     /locus_tag="VF357_09525"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7524431.1"
                     /translation="MTTDGRFAPSNRAAGVQLPIIPIVAGWIAETPGTISLGQGVVHY
                     GPPPAALTAIPEFLATIPHHQYIPDAGLPELRNAFEGKLRAENGIDAPFERRIYVTAG
                     ANQAFLNAVLAICDPGDEVILLSPYYFNHEMAITLASAVPVPVPVDERLQPDLPAIAA
                     AIRERTRAIVTVSPNNPTGAVYPQETLAAIHRLCAERGIYHVSDEAYEYFTYDGALHF
                     SPGSLGGEHVISLYSLSKAFGMASWRVGFLVAPEHLNRDLMKIQDTVVVSGSAISQFV
                     GLHAMREGRGYCAAHLPSLARVRKEVLARLSAIPDLVEVPPVTGAFYLFVKVNAGMSA
                     MTLSERLVREHKVAVIPGETFGVRRGCWLRIAYGSLREKTAVEGIDRLITGLRTILAP
                     "
     gene            complement(1442..>2250)
                     /locus_tag="VF357_09530"
     CDS             complement(1442..>2250)
                     /locus_tag="VF357_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7524432.1"
                     /translation="FGCGSLLMMAILFRRSAKPVPARADWRRLAVVALVGVTGFNTLF
                     FRGLSLAPASDAGMIIPTMSPVFTAMAGMLFLRERVRFWRVAGLTVSLAGAVLFFWAV
                     IVHPAGQGGRVWGDAALMGAAMCWAAASILSRPLSVRMGAMPSAAWTIFLGSLVLLLV
                     SSPKLAAVPWGELTGRFWIVLAYVVIFPTVIAYILWMEGIRAIGSGPTTSFMFLAPVF
                     ALLIAAALLGERPTALQGAGGALMLFGVWLVNRPGEGGTPVTGRGSSASP"
BASE COUNT          397 a          809 c          732 g          312 t
ORIGIN      
        1 tcgcccggac ggacggttca cactcctcct caagcaccgc cctcaactgg acccggccac
       61 gggagggaaa ttcgatctgc agctctcggg tcatacccac gatgggcagg tctttccgtt
      121 ccgcctcctc acccgcctgg ccttccccct gctcggggga gaccacccgg tcccgggggg
      181 cgggatcctg cacgtgagcc gaggcacggg aacgtggggg cccccgatgc ggttcctcgc
      241 ccctccggag gtcaccattg tggagatcga gcgacgcact ccatccggtc cctcgtgacc
      301 accgacggcc gcttcgcccc ctcgaaccgc gcggcggggg tccagcttcc catcatcccg
      361 atcgtggccg ggtggatcgc ggagacgccg ggtacgatct cgctgggaca gggcgtggtc
      421 cactacggac cgccgccggc ggcgctcacg gcgatccccg agttcctcgc gaccattccc
      481 caccaccagt acatccccga cgccggtctc cccgagcttc ggaatgcgtt cgaagggaaa
      541 ctgcgcgccg agaacgggat cgacgccccc ttcgagcgcc ggatctacgt gacggccggc
      601 gcgaaccagg cgttcctcaa cgcggtcctc gcgatctgcg accccgggga cgaggtgatc
      661 ctcctctccc cgtactactt caaccacgag atggcgatca ccctcgcctc ggcggttccc
      721 gtgcccgtcc ccgtcgacga acgactgcag ccggaccttc cggcgatcgc cgcggcgatc
      781 agggaaagga cgcgcgcgat cgtcaccgtg tcgccgaaca accccaccgg ggcggtctac
      841 ccgcaagaga cgctggcggc gatccaccgg ctctgcgcgg agcgcgggat ctaccacgtc
      901 agcgacgagg cgtacgaata tttcacgtac gacggcgccc tccacttctc ccccggctcg
      961 ctcggcgggg agcacgtaat ctccctctac agcctctcca aggcgttcgg gatggcgagc
     1021 tggcgggtcg ggttcctcgt ggcgccggag cacctgaacc gcgacctcat gaagatccag
     1081 gacacggtcg tcgtcagcgg ttcggcgatc tcccagttcg tggggctgca tgcgatgcgc
     1141 gaagggcgcg ggtattgcgc ggcgcacctc ccttccctcg cgcgggtgcg gaaagaagtc
     1201 cttgcgcgtc tatcggccat ccccgacctg gtggaagttc cccccgtgac gggcgccttc
     1261 tacctgttcg ttaaggtgaa tgccgggatg agcgccatga ccctgtcgga acgactcgtg
     1321 cgagagcaca aggtggccgt catccccgga gagacgttcg gcgtgaggcg ggggtgctgg
     1381 cttcggatcg cctacggaag cttgcgcgag aagaccgccg tcgaaggaat cgaccggttg
     1441 atcacgggct tgcggacgat cctcgccccg tgaccggcgt ccccccttct ccgggacggt
     1501 ttaccagcca tacaccgaac agcatcagtg caccgcccgc cccctgcagc gccgtgggcc
     1561 gctcgccgag cagcgcggcg gcgatcagca gggcgaagac gggcgcaagg aacatgaacg
     1621 acgtcgtcgg cccggacccg atcgcccgga tcccctccat ccacaggatg tacgcaatca
     1681 ccgtggggaa gatcaccacg tacgccagga cgatccagaa gcggcccgtg agttcccccc
     1741 acggtacggc ggcaagcttg ggggaggaga ccagcagcag gacgaggcta ccgaggaaga
     1801 tcgtccacgc ggccgagggc atcgcgccca tccgcaccga cagcggacgc gacaggatgg
     1861 aggccgccgc ccagcacatc gcggccccca tcagcgccgc gtccccccac acccgacccc
     1921 cctgccccgc ggggtgcacg atcacggccc agaagaagag gaccgcaccg gcgagggaaa
     1981 cggtgagacc ggcgacccgc cagaagcgga ctctctcgcg aaggaagagc atgccggcca
     2041 tcgcggtgaa gacgggggac atcgtgggga tgatcatccc cgcgtcggag gcgggcgcaa
     2101 gagacagccc gcggaagaaa agggtgttga accccgtgac gccgaccagc gcgacgacgg
     2161 cgagtcgcct ccaatcggct cgcgcgggga cgggcttcgc cgaccggcgg aagaggatcg
     2221 ccatcatcag cagggaaccg cagccgaacc
//
LOCUS       DASVJL010000454         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1581217, whole genome shotgun sequence.
ACCESSION   DASVJL010000454 DASVJL010000000
VERSION     DASVJL010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581217"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1832
                     /locus_tag="VF357_09535"
     CDS             156..1832
                     /locus_tag="VF357_09535"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEX7524433.1"
                     /translation="MADDPGTIRFLPDGIDVPGVPGESLLSHALRGGVPLLSPCGGES
                     RCGKCRVQVAGETRGGEDPGTLTRAEINAGIRLACRCFPSSGDVSVTILPGSRPARLA
                     AYLNGKDLAGDEPFHPLSRAASGGVPLGLALDLGTSTLAGALVDLSDGTVLSRATADN
                     PQMACGEDLISRVVFAEETTGGFELVRGRLLSGVNGLVRRLLEASPVSGEITDVVAAG
                     NTVISHFLYGISPSPIRRPPYHPVRKEYPAVPGETLGTGPATARAMWRIFPSVGGFVG
                     GDVVAGILASGMHRREEVSLLFDVGTNGEVALGNRDWRIVCSSSAGPAFEGGEVGCGM
                     RAYPGAIESVRIKPGSAAAEWTVIGRTLPAGLCGSGILDLCAELFRAGIVDRSGRFTS
                     RGGDGRRSTERGEAFVVVPGEKSATGRDVVFCESDLKSVLRTKAALCAAAEALLAAVG
                     LPREAVARVYIAGGFGNFLDLRSALAIGLFPPIPLARFVPLGNASLAGALGALRNRRR
                     WKEALSLTSGTVYHDLSSDPGFMELYQRGLFLPHTDEESYRSVLAAEVTQ"
     gene            1829..>2281
                     /locus_tag="VF357_09540"
     CDS             1829..>2281
                     /locus_tag="VF357_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524434.1"
                     /translation="MIALPGLFPCTGVGSLPHLDPRAAVAEVLSRFREIPYWPQLPRR
                     TPLEHMYPQFAAALPGASVVGERLTMKSGEELLPDAEAFYETFLSGDLSPFAVPAERA
                     AGLHALMEAGVGPFPAVKGQVTGPVSFGLMVCDREKKPVFYDPVGRDVL"
BASE COUNT          285 a          814 c          807 g          375 t
ORIGIN      
        1 gacccaccgg acccttatcc ttcccgggat ggtcgcgcgc ctctccggcc ggatcgagga
       61 gctgtccggg tggcgggtcc tcgtcggccc gcaggagtcg tccgccctcc cgtcgtacct
      121 tcgccgactc ccaccggcgg cattcctccc agttcatggc tgacgacccg gggacgatcc
      181 gcttccttcc cgacgggata gacgtccccg gagtccccgg ggaatcgctt ctttcccacg
      241 ccctgcgcgg cggtgtcccg cttctctcgc cgtgcggcgg ggagagccgg tgcggaaaat
      301 gccgggtcca ggttgccggc gaaacgcgcg gcggggagga tccggggacg ctcacgcggg
      361 cggaaatcaa tgcggggatc cgcctcgcgt gccgatgctt cccctcctcc ggcgacgtct
      421 ccgtgacgat ccttcccgga agcaggcccg cccgcctcgc ggcgtacctg aacggaaagg
      481 acctggcggg ggacgaaccg ttccaccccc tgtcccgcgc cgcatcggga ggcgttccgc
      541 tcggattggc gctggacctg gggacgagca ccctggccgg cgcgctcgtg gatctctcgg
      601 acggaaccgt cctgtcccgc gcgacggcgg acaacccgca gatggcgtgc ggcgaggacc
      661 tcatctcccg cgtggtgttc gccgaagaga cgaccggcgg gttcgaactg gtgcggggcc
      721 ggctcctttc gggggtgaac ggcctggtgc ggcgactcct cgaagcctcc ccggtgtcgg
      781 gggaaatcac cgacgtcgtc gccgcgggga acaccgtcat ctcccacttc ctgtacggga
      841 tctccccctc cccgatccgg cggccgccgt accatccggt acggaaggag taccccgccg
      901 tgccggggga aaccttgggg accggtccgg cgacggcgcg ggcgatgtgg aggatcttcc
      961 cgtccgtcgg ggggttcgtc ggcggcgacg tcgtggcggg cattctcgct tcggggatgc
     1021 accggcggga ggaggtcagc cttctcttcg acgtcggcac caacggggag gtggcgctgg
     1081 ggaaccgcga ttggcggatc gtctgctcct cctccgccgg gcccgccttc gagggaggcg
     1141 aggtcggctg cggcatgcgt gcgtaccccg gagcgatcga gtcggtccgg atcaaaccgg
     1201 ggagcgcggc ggcggagtgg acggtgatcg ggcggacctt gccggcgggc ctgtgcggct
     1261 cgggaatcct cgatctgtgc gccgagctgt tccgggccgg gatcgtcgat cgctcgggac
     1321 ggttcacttc ccgaggtggc gacgggcgcc gctccacgga gcgcggcgaa gcgttcgtcg
     1381 tggtccccgg ggagaagagc gccacgggcc gcgacgtggt cttctgcgag tcggacctga
     1441 aaagcgtcct gcggacgaaa gccgcccttt gcgcggccgc cgaggcgctg ctcgccgcgg
     1501 ttgggctccc gcgggaggcg gtggcgcgcg tctacatcgc gggggggttt ggcaactttc
     1561 tcgacctgcg ctccgccctt gccatcgggc tgttcccccc catcccgctc gcccggttcg
     1621 tcccgctggg aaacgcgtcg ctggccggcg cgctcggcgc gctgcgcaac cggcggcgct
     1681 ggaaggaggc gctttcgctc acctccggga cggtgtacca cgacctctcc tccgatcccg
     1741 gcttcatgga gctgtaccag cgtggtctct tcctccccca caccgacgag gagagctacc
     1801 gctccgtgct cgccgcggag gtgacccaat gatcgcgttg cccggactct tcccgtgcac
     1861 gggcgtggga tccttgccgc acctcgatcc ccgcgccgcc gtggccgaag tcctgtcccg
     1921 cttccgggag atcccgtact ggccccagct cccgcgccgg accccgctcg aacacatgta
     1981 cccgcaattc gccgcggccc tccccggggc ttcggtcgtc ggggagcgcc tgacgatgaa
     2041 aagcggggag gagcttctgc ccgacgcgga ggcgttctac gagacgttcc tctccggcga
     2101 tctctccccc ttcgccgtcc ccgcggagcg cgcggcggga ctgcacgcgc tgatggaggc
     2161 gggcgtcggc ccctttcccg cggtgaaagg ccaggtgacc gggcccgtat ccttcgggct
     2221 catggtatgc gaccgcgaga agaaaccggt cttctacgac cccgtgggcc gggacgtcct
     2281 g
//
LOCUS       DASVJL010000455         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1111810, whole genome shotgun sequence.
ACCESSION   DASVJL010000455 DASVJL010000000
VERSION     DASVJL010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111810"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VF357_09545"
     CDS             <1..413
                     /locus_tag="VF357_09545"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEX7524435.1"
                     /translation="LEIPLKIALERISSSLPPERFDGWKEGYCPVCGSRAGMAELSGD
                     EGKRRLSCSACFFRWPYPRIQCPYCGNADPETLSYFSAGDGPTRVGVCRKCSRYLKTR
                     DARLGNADVPLEAEDLATLHLDLLAGKEGFERGK"
     gene            410..1804
                     /gene="fdhD"
                     /locus_tag="VF357_09550"
     CDS             410..1804
                     /gene="fdhD"
                     /locus_tag="VF357_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX7524436.1"
                     /translation="MSRLPGDGAKGGDAPPVLRYDGRQLAHARHLPVREVPVALTVNG
                     VALATLIASPHELHFLVAGFLRMQGLVRSAGDLLTLSVCEDFGAASVRIRGNVPDRII
                     PTFTSGCGAGISFHVPTAGGRQVQLPSAGPLVSPESLFSAMDALARASEAYRSSGGIH
                     SAGAWDGERLLLFSEDIGRHNTIDRIAGQALLGGIDLSGRILVASGRISSEMAAKAGS
                     LGISVIASRTSPTDFAVRICGELGITLVGYVRGRRFNVYTHPTRIAAAAAERIPGVTG
                     VILAGGRSGRMGSDKALLPYQGGRFIEAIHRRMEELFDEVIVATGETARYDFLPCRRV
                     TDLYPGMGPLGGIHAALRASGSEKIFVVACDMPHFAPDLIRHLCSLAEEVDVVVPEGE
                     GGLEPLHAVYRKGVLPAVEDALRDGQGRVVSFFDRVRVRRVSLAEVERIDPSLAAFRN
                     INTPEEYYRFRDGG"
     gene            1821..2108
                     /locus_tag="VF357_09555"
     CDS             1821..2108
                     /locus_tag="VF357_09555"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX7524437.1"
                     /translation="MEGPKNPMEIQVTFPEHLKGGVYSNNMTVTHTREEFVLDFLMVA
                     PPAGSVTARVILSPGHAKRILAALSENIRKYEEAHGTIFTAEEPQGKIGFN"
     gene            complement(2129..2914)
                     /locus_tag="VF357_09560"
     CDS             complement(2129..2914)
                     /locus_tag="VF357_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX7524438.1"
                     /translation="MSEIVQVGRNGGVATVRMNRPERLNAYNQEMGEALLSAVSEVSA
                     DPSVRCVVLTGTGKAFSAGGDVESFAAFENEGPGKFMGLAIGLHALIATLRRAPKPVV
                     GAVNGVAAGAGFSMALACDVAVAASSARFTLGYQNIGLSPDGGMTFFLARAVGTQRAM
                     EMTLFSRVLPAAQAAEWGLVQEIFPDAEFASGVDALADRLASGPTLAYSRAKELYNRA
                     LSQPLETQLEEERQNISRCAGSRDFREGIRAFLEKRPARFEGR"
BASE COUNT          486 a         1092 c         1035 g          512 t
ORIGIN      
        1 tcctcgagat tccgttgaag atcgccctcg agcgcatttc ctcctctctt cccccggaac
       61 ggttcgatgg ctggaaggaa gggtattgtc ccgtgtgcgg ctcccgcgcc gggatggctg
      121 aactttccgg ggacgagggg aagcggcggc tctcctgctc cgcctgcttc ttccgctggc
      181 cgtaccctcg gatccaatgc ccctattgcg ggaacgccga cccggaaacg ctctcctatt
      241 tcagcgcggg ggacggcccc acccgggtcg gggtgtgccg caaatgcagc cgatacctga
      301 agacgcgcga cgcacgcctc gggaacgcgg acgtgccgct ggaagccgaa gacctcgcca
      361 ccctccacct cgacctgctg gccgggaagg aagggttcga gagggggaaa tgagccgcct
      421 tcccggagac ggcgcgaagg gcggagacgc ccctcccgtc ctgcggtacg acggacgcca
      481 gctggcgcat gcccgccatc tccccgtgcg ggaggtcccc gttgccctca cggtcaacgg
      541 tgtcgccctc gcgacgttga tcgcctcgcc ccacgaactg catttcctgg tggccggttt
      601 cctccggatg caggggttgg tccggtccgc cggcgatctc ctgaccttga gcgtttgcga
      661 agacttcggc gcggcgtccg tccggatccg gggaaacgtg ccggatcgca tcattccgac
      721 cttcacctcg gggtgcggcg cggggatcag cttccacgtg cccaccgccg gggggcgcca
      781 ggtccagctc ccctccgcgg ggccgctcgt ttccccggag tcgctttttt cggcgatgga
      841 cgcgctcgcg cgggcgtccg aggcgtaccg atcgagcggc gggatccact ccgccggcgc
      901 atgggacggc gaacgactcc tcctgttttc cgaggacatc gggcggcaca acacgatcga
      961 ccggatcgcg gggcaggcgc tcctgggcgg gatcgacctc tcgggcagga tcctcgttgc
     1021 gtcgggacgc atttcctccg agatggcggc aaaggcgggt tccctcggga tctccgtgat
     1081 cgcttcccgc acctctccca ccgacttcgc cgtcaggatc tgcggggagc tggggatcac
     1141 cctcgtcggg tacgtgcggg gccgcaggtt caacgtctac acccatccga cgcggatcgc
     1201 cgccgcggcc gcggagagga tccccggggt cacgggggtg attctcgcgg gggggcgctc
     1261 cggccggatg ggaagcgaca aggcgctcct gccgtaccag ggcgggcggt tcatcgaggc
     1321 gatccaccgg cggatggagg agctgttcga cgaggtgatc gtcgccaccg gggaaacggc
     1381 ccggtacgat ttcctcccat gccgccgggt gacggacctc taccccggga tgggcccgct
     1441 gggggggatc cacgcggcgt tgcgggcgag cggatcggag aagatcttcg tcgtggcgtg
     1501 cgacatgccg cacttcgcgc cggacctcat ccgacacctg tgctccctcg cggaagaggt
     1561 ggacgtggtg gttcccgagg gggagggggg gctggagccg cttcacgcgg tgtaccggaa
     1621 gggcgtcctc cccgccgtgg aagacgcgct gcgggacggg cagggccgcg tggtctcctt
     1681 cttcgaccgg gtgcgggtcc gccgcgtttc cctcgcggag gtggagcgga tcgaccccag
     1741 cctggccgcc ttccggaaca tcaatacccc cgaggagtat taccgctttc gggacggggg
     1801 gtaaccgacg ggagggcacg atggaaggac cgaagaatcc gatggagatc caggtgacgt
     1861 tcccggaaca tctcaagggc ggcgtctact ccaacaacat gaccgtgacc cacacccgcg
     1921 aggagttcgt gctggatttc ctgatggtcg cgccccccgc cggctccgtc accgcgaggg
     1981 tcatcctctc ccccggccac gccaaacgga tcctcgcagc gctctcggaa aatatcagga
     2041 agtacgagga ggcgcacggg acgatcttca cggccgagga gccgcagggg aagatcggct
     2101 tcaactgaga ggggggggaa atccccggtt atctcccctc gaaccgggcg ggccgctttt
     2161 ccaggaacgc ccggatcccc tcccggaaat cccggctccc cgcgcaccgg gagatgttct
     2221 gccgctcctc ctcgagctgc gtctccagcg gctgggacaa cgcccggttg tatagttctt
     2281 tcgccctgga gtaggcgagc gtggggccgg aggcgagccg gtccgccagt gcatcgaccc
     2341 cggaggcgaa ctccgcgtcc gggaagatct cctgtaccag cccccactcg gcggcctggg
     2401 ccgccgggag gaccctcgaa aagagcgtca tctccatcgc ccgctgcgtc cccaccgcgc
     2461 gggcgaggaa aaaggtcatc cccccgtcgg gggagagccc gatgttctgg tatccgagcg
     2521 tgaagcgggc ggacgaggcg gccacggcga cgtcgcacgc caacgccatc gagaatccgg
     2581 ccccggcggc cacaccgttg accgccccga cgaccggctt cggggcgcgc cgcagcgtcg
     2641 cgatcagggc gtgcaggccg atcgccagcc ccatgaactt ccccggcccc tcgttctcga
     2701 acgccgcgaa cgactccacg tcgccgccgg cggagaacgc cttcccggtt ccggtgagga
     2761 ccacacaccg gaccgacggg tccgccgaga cttccgaaac cgccgacagg agcgcctccc
     2821 ccatctcctg gttgtaggcg ttcaaccgct ccggccggtt catccggacc gtcgcgaccc
     2881 ccccattccg ccccacctgg acgatttcgc tcatttccgc cccctctatc ttctctccgc
     2941 gtcgaccgat agatttcgcc gccggttcgt tccatcggcg gcaccgtacc aatataatga
     3001 ataaatagcc acggcttacc atttcgtcgc cgccttggta ttcttccatt gtttcccggc
     3061 ccgcggacgc gggcgaaagg agagccatgc cgacgctgtt cgaaaagatc cgggaccgcc
     3121 acgtc
//
LOCUS       DASVJL010000456         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1207094, whole genome shotgun sequence.
ACCESSION   DASVJL010000456 DASVJL010000000
VERSION     DASVJL010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207094"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VF357_09565"
     CDS             <1..1139
                     /locus_tag="VF357_09565"
                     /inference="COORDINATES: protein motif:HMM:NF028290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX7524439.1"
                     /translation="EYASFLILLASLFTISGGILLRGTLRGSVGVNCAILAVGAVLAN
                     IFGTTGASMLLIRPLLRANAHRHRVGHVVVFFIFVVANIGGALTPIGDPPLFLGYLRG
                     VPFFWTLRAMGLLWAVACALVIGAFYLVDRRALALETVAVAYGGPALRAAPAEATESG
                     ASLPASALGRPWPSDVGGGEARVPLSIEGKINLPLLMVVIGAVFLPTPWREMAMVAAA
                     AVSAWKTPAKVREENEFTWYPIEEVAVLFAGIFATMIPALLILKARGAELGVSTPAHF
                     FWAAGALSSFLDNAPTYLTFFSLAQGVGGAQTVAGVSAPLLQAISAGAVFMGANSYIG
                     NAPNFMVKAIAEEAGVRMPSFFGYMAWSCGILLPIFVLLTLIFF"
     gene            1179..>2033
                     /locus_tag="VF357_09570"
     CDS             1179..>2033
                     /locus_tag="VF357_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7524440.1"
                     /translation="MGKMALGTVRSLSVKCLSELSWHDNARMRLDVRDAGGLSADQFD
                     VKWTAGNAAGVSALLSMEDAEGHARTILMDAGWDVEYMDGVFRREGVDRLLADGKIEF
                     VYITHEHVDHFWGMPAVTKYRPDVKVVIPAGFSARSKELLKSSGHRGEVVEMAPGPHL
                     LFPGCASVTFDQPIFLKTHGEEVLYVNVERKGIVTITGCCHPGVIGLLEYAKENFDGF
                     SAFLGVYGGLHISPFEEWGPPQEEVLDKLQAFNVRKFACNHCTGETAVRKMRERGMPV
                     TGGTARHGS"
BASE COUNT          290 a          658 c          722 g          363 t
ORIGIN      
        1 tcgaatacgc ctccttcctc atcctcctcg cgagcctctt cacgatctcg ggggggatcc
       61 tcctgcgcgg gacgctgcgc ggctccgtgg gggtcaactg cgcgatcctg gccgtcgggg
      121 cggtgctcgc gaacatcttc gggacgacgg gcgcctcgat gctcctcatc cgtccgctgc
      181 tgcgggccaa cgcgcaccgg catcgggtgg gccacgtcgt cgtcttcttc atcttcgtgg
      241 tggccaacat cggcggggcg ctcacaccga tcggcgatcc gcccctgttc ctcggctacc
      301 tgcggggggt gccgttcttc tggacgctgc gcgcgatggg cctgctgtgg gccgtcgcgt
      361 gcgccctggt gatcggcgcc ttctacctgg tggaccgccg ggcgctcgcg ctggaaacgg
      421 tggcggtggc ctacggcggt ccggctctga gggccgcgcc ggccgaggcg acggaatccg
      481 gggcttccct gcctgcctcg gcacttggcc gtccatggcc gtcggacgtt gggggcgggg
      541 aagcgcgggt tccgctgtcg atcgagggga aaatcaacct gccgctgctg atggtcgtga
      601 tcggggccgt cttcctgccg accccgtgga gggagatggc gatggtggcg gcggcggccg
      661 tctccgcgtg gaagaccccg gcgaaggtgc gggaggagaa cgagttcacc tggtacccga
      721 tcgaggaggt ggcggtcctg ttcgccggaa tcttcgccac gatgatcccc gcgctgctga
      781 tcctcaaggc gcgcggggcg gagctgggag tgtcgacgcc ggcccacttc ttctgggcgg
      841 cgggggcgct gtcgagcttc ctcgacaacg cgccgaccta cctcaccttc ttcagcctcg
      901 cgcagggggt ggggggggcg cagacggtgg cgggggtgtc ggccccgctc ctgcaggcga
      961 tcagcgcggg cgcggtcttc atgggggcga actcgtacat cggcaacgcc ccaaacttca
     1021 tggtgaaggc gatcgccgag gaggcgggcg tccggatgcc ctccttcttc ggctacatgg
     1081 cctggtcctg cggcatcctc ctcccgatct tcgtccttct caccttgata ttcttttgag
     1141 ttaatatagc ggcagcacgg cgagggggag gtggggcgat ggggaagatg gcgttgggga
     1201 ccgtaaggtc gctttccgtg aagtgcctct ccgagttgtc gtggcacgac aacgcgcgga
     1261 tgcggctgga cgtccgcgat gccggtgggc tgtccgccga ccagttcgac gtcaagtgga
     1321 cggcggggaa cgccgccggg gtctccgccc tcctgtccat ggaggatgcc gaagggcacg
     1381 ccaggacgat cctgatggac gcgggctggg acgtcgaata catggacggc gtcttccgcc
     1441 gcgagggggt cgaccggctc ctcgccgacg gaaaaatcga attcgtctac atcacccacg
     1501 aacacgtgga ccatttctgg gggatgcccg ccgtcacgaa gtaccgcccc gacgtgaagg
     1561 tcgtcatccc cgccggattc tccgcccgca gcaaggagtt gctgaaatct tccggccaca
     1621 ggggggaggt cgtcgagatg gcgcccggcc cgcacctgct cttccccggt tgcgcctccg
     1681 tcaccttcga ccagccgatc tttctcaaga cccacggaga ggaggtcctc tacgtgaacg
     1741 tggagaggaa ggggatcgtc accatcaccg gctgctgcca ccccggggtg atcgggctgc
     1801 tcgaatacgc gaaggagaac ttcgacgggt tctccgcctt tctcggcgtc tacggcggcc
     1861 tgcacatctc cccgttcgag gagtggggcc cgccgcagga agaggtgctc gacaagctgc
     1921 aggcgttcaa cgtccggaag ttcgcctgca accattgcac gggcgagacg gcggtgcgca
     1981 agatgcggga gcgcggcatg cccgtgaccg gcggcacggc gcgccacggc agc
//
LOCUS       DASVJL010000457         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1018030, whole genome shotgun sequence.
ACCESSION   DASVJL010000457 DASVJL010000000
VERSION     DASVJL010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018030"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VF357_09575"
     CDS             <1..826
                     /locus_tag="VF357_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524441.1"
                     /translation="LATEFPHLGFELLLLRLANAQGLLSVEELGSSAEAAGATGGKEA
                     PKLPTDRPAAPPAADGHGRPSVGERRDASGYPSPTTFSRKPAPPPKAMDGLVPRQAGM
                     PQDLQPRPEAGSAKGASAKGDPGLWDAVRRNLEGKKKTVLLGLLSQMRGELQEGEFVI
                     TCGHEMMLDRLKEKDKWQPLLTALEEAAGRAVPVRLSVSTEKKSPEPDAVAPGDAGLE
                     RKALEEPTVLEILRTFEGSILVKVQPAPPVEVPRNEVAADEDAGEPEIPEPVEEGG"
     gene            823..1134
                     /locus_tag="VF357_09580"
     CDS             823..1134
                     /locus_tag="VF357_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX7524442.1"
                     /translation="MTDFKDLMQQAQEVRDRFQRLQEELGGRTVEGSAGGGMVVAVMN
                     GRQELLSVRIEKEVVSPDDVGMLQDLVRAAVNDALARSRELAAAEMSKVTGGMLPPGI
                     L"
     gene            1131..>1577
                     /locus_tag="VF357_09585"
     CDS             1131..>1577
                     /locus_tag="VF357_09585"
                     /inference="COORDINATES: protein motif:HMM:NF025044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX7524443.1"
                     /translation="MTAVYPKPLRRLILLLSRLPGIGEKTATRLSMFLLKMPPEFVRE
                     LGAAIAGIPESVTKCSKCFNIADEDPCAFCTDPARRDDLICVVESPTDIVPIEKSGEY
                     KGRYHVLGGAISPIDGVMPDDLRVRELLERTARGGVSEVILATNLTA"
BASE COUNT          266 a          507 c          579 g          225 t
ORIGIN      
        1 gctggcgacc gagttcccgc acctcgggtt cgagctgctc ctgctgcgcc tcgccaacgc
       61 ccaggggctg ctctccgtcg aggagctcgg ctcgtccgcc gaagcggcgg gagcgaccgg
      121 gggcaaagag gccccgaagc tgcccaccga tcgccccgcg gcacccccgg cggccgatgg
      181 ccatggacgg ccaagtgtcg gggagcgcag ggatgcgtcg ggttatccct ccccgacgac
      241 gttctcccgc aagccggccc ccccgccgaa ggccatggat ggcctggtgc cgaggcaggc
      301 agggatgccc caggatctgc agcctcggcc ggaggcgggc tccgcgaaag gcgcgtcggc
      361 gaagggggac cccggccttt gggatgccgt gcggcgcaac ctcgagggga agaaaaagac
      421 cgtcctgctc ggcctcctct cccagatgag gggggagctt caggagggcg aattcgtcat
      481 cacctgcggc cacgagatga tgctcgaccg gttgaaggag aaggacaagt ggcagccgct
      541 cctcaccgcc ctcgaggaag cggccgggcg cgccgtgccg gtccgattgt cggtttccac
      601 ggaaaaaaaa agtccggagc ctgacgccgt agccccgggg gacgccggcc tcgagcggaa
      661 agcgctcgaa gagccgaccg tcctcgagat tttgcggacc ttcgagggat cgatcctggt
      721 caaggtgcag cccgcgcctc cggtcgaggt cccgcggaac gaagtcgcgg cggacgagga
      781 tgcgggggag ccggagatcc cggagccggt cgaggagggg ggatgacgga cttcaaggac
      841 ctgatgcagc aggcgcagga ggtgcgcgac cggttccagc gcctccagga ggagctgggg
      901 gggcgcacgg tcgaggggtc ggcgggcgga gggatggtgg tggccgtcat gaacggccga
      961 caggagctcc tctccgtgcg catcgagaag gaagtggtct cccccgacga cgtggggatg
     1021 ctgcaggacc tggtccgcgc ggcggtgaac gacgccctcg cgcgctcccg ggagttggcg
     1081 gccgccgaga tgtcgaaggt cacgggcggg atgctgccgc cgggaatcct gtgaccgcgg
     1141 tctatccgaa accgctgcgc cgcctcatcc tcctcctctc ccgcctcccc gggatcgggg
     1201 agaagacggc cacgcgcctg tcgatgttcc tcctgaagat gccgcccgag ttcgtccgcg
     1261 agcttggggc ggccatcgcc gggatcccgg aatccgtgac aaaatgttcg aaatgtttta
     1321 acatagctga cgaggacccg tgcgctttct gtacggaccc cgcgcgccgg gatgacctga
     1381 tctgcgtcgt ggaaagtccc accgatatag tcccgatcga aaaaagcggg gagtataagg
     1441 ggaggtacca cgtcctcggc ggggcgatct cgccgatcga cggcgtcatg ccggacgacc
     1501 tccgggtgcg ggagctcctg gagcggacgg cccgcggcgg cgtttccgag gtgatcctgg
     1561 cgacgaacct cacggcg
//
LOCUS       DASVJL010000458         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1581745, whole genome shotgun sequence.
ACCESSION   DASVJL010000458 DASVJL010000000
VERSION     DASVJL010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581745"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VF357_09590"
     CDS             complement(<1..461)
                     /locus_tag="VF357_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524444.1"
                     /translation="MLGALKKKGYRKVLKTLRDGKAIWLTNTGPRDTGQSDFKNRFTL
                     HDLSRLMPVYDASGYFSIEVHGGARLHQNLMNNMIHPFEEARLFSARMPNVLTQTLIR
                     STNVWGYRMYPRNIVRLAVESFLPAIDVWRCFDFLNYVPNMAPVAEEVLRGG"
     gene            complement(472..>1536)
                     /locus_tag="VF357_09595"
     CDS             complement(472..>1536)
                     /locus_tag="VF357_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524445.1"
                     /translation="SASGVPVTPAIEDIEDVDQVIKFGDRVGWPLLVKASAGGGGKGM
                     QKLASPYEVEEGLASARSVAEKAFGMGTAFVEKFIEGGRHIEFQFLADRYGNVIHLGE
                     RECSIQRRHQKLVEEAPSSLLTSEVRAKMGAVVVKAMKDIGYETAGTLEFLVDKSGKF
                     YALEVNTRIQVEHTVTEMITGFDIIRKMYKIAAGERLSLRQQDVLLRGHAIQCRINAE
                     DPKNGFAPSFGRITFLRQISGPFIRTESGIYQGWEVPSFYDSLLAKICSVGKDRGTAI
                     QRMRRALREYDIWGVRTTIPLLRRIMDQPDFVAGKIHTGFIDENIAGLTEYEEVEEEI
                     YKAARFVAEVSGLGRNVHSG"
BASE COUNT          244 a          514 c          494 g          284 t
ORIGIN      
        1 ccgccgcgaa gcacctcctc ggcgaccggc gccatgttcg ggacgtagtt caggaagtcg
       61 aagcagcgcc acacgtcgat cgcggggagg aacgactcga ccgccagccg cacgatgttg
      121 cgcgggtaca tccggtaccc ccagacgttc gtcgaccgga tgagcgtctg ggtcagtacg
      181 ttcggcatcc gcgcggagaa gagccgcgcc tcctcgaacg gatggatcat gttgttcatc
      241 aggttctggt ggaggcgggc cccgccgtgg acctcgatgg agaagtaccc cgaggcgtcg
      301 tagaccggca tcaggcggga caggtcgtga agggtgaacc ggttcttgaa gtcgctctgg
      361 ccggtgtcgc gcggccccgt gttcgtcagc cagatcgcct tgccgtcccg gagggtcttg
      421 aggaccttcc ggtacccttt cttcttcagg gcgccgagca tcttcccccc gtcacccgct
      481 gtgaacgttg cggccgaggc cggagacctc ggcgacgaag cgggccgcct tgtagatctc
      541 ctcctcgacc tcttcgtatt cggtgagccc ggcgatgttc tcgtcgatga acccggtgtg
      601 gatctttccg gccacgaagt cgggctggtc catgatccgc cgcagcagcg ggatcgtcgt
      661 ccggacgccc cagatgtcgt actcccgcaa ggcacgccgc atccgctgga tcgccgtccc
      721 ccggtccttc cccacggagc agatcttcgc gagcagggag tcgtagaacg aggggacctc
      781 ccacccctgg tagattcccg actcggtccg gatgaagggg ccgctgatct ggcgaaggaa
      841 ggtgatccgc ccgaaggagg gggcgaagcc gttcttcggg tcctcggcgt tgatgcggca
      901 ctggatcgcg tggccgcgca ggagaacgtc ctgctgccgc agcgacaggc gttcgcccgc
      961 ggcgatcttg tacatcttcc ggatgatgtc gaacccggtg atcatctcgg tgacggtgtg
     1021 ctccacctgg atccgcgtgt tcacttcgag ggcgtagaac ttgccgctct tgtcaacgag
     1081 aaactcgagc gtaccggccg tctcgtaccc gatgtccttc atcgccttga cgaccaccgc
     1141 tcccatcttc gcccggacct cggaggtcag cagggagctg ggcgcctcct ccaccagctt
     1201 ctggtgacgc cgctggatgg agcattcccg ctcgcccagg tggatgacgt tgccgtaccg
     1261 gtccgcgagg aactggaact cgatgtggcg gccgccctcg atgaactttt cgacgaaggc
     1321 ggttcccatg ccgaacgcct tctcggccac ggagcgcgcg gaggccagcc cctcctccac
     1381 ctcgtacgga gaggcgagtt tctgcatccc cttcccgccc ccgccggcgg acgccttgac
     1441 gaggagcggc catccgaccc gatcgccgaa tttgatcacc tggtcgacgt cctcgatatc
     1501 ctcgatggcg ggggtgaccg gcacgccgga agccga
//
LOCUS       DASVJL010000459         3687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_267335, whole genome shotgun sequence.
ACCESSION   DASVJL010000459 DASVJL010000000
VERSION     DASVJL010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267335"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /gene="groEL"
                     /locus_tag="VF357_09600"
     CDS             <1..181
                     /gene="groEL"
                     /locus_tag="VF357_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX7524446.1"
                     /translation="EFEDLMKGGILDPTKVTRVALQNAASVAGLMITTECAIAEKPEE
                     KKEMPQMPPGGGGMY"
     gene            266..598
                     /locus_tag="VF357_09605"
     CDS             266..598
                     /locus_tag="VF357_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX7524447.1"
                     /translation="MPIHEYKCRKCEEVVERIEGMHDKPMKKCLSCGGKVDQMISSSA
                     FVLKGTGWYATDYGTKSHDNGNGKGKGNGNGNGKGEPKAKEAAEASCPAASGAGAPAA
                     CAGCPKLD"
     gene            888..1748
                     /gene="folD"
                     /locus_tag="VF357_09610"
     CDS             888..1748
                     /gene="folD"
                     /locus_tag="VF357_09610"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF010785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX7524448.1"
                     /translation="MTAKLIDGKAIAASVRAGVKERVAEFTARTGIRPGLTVVLVGEN
                     PASQVYVRNKGKAASEAGFLSRQIDLPAVTKESELLDLVARLNADDTVHGILVQLPLP
                     GQIDESKVIEAIDPAKDVDGFHPVNAGRLFTGGTSFLPCTPYGILTMLDYEKVELKGK
                     HAVVVGRSNIVGKPVAILLLSRHATVTICHSRTVDLPSVVRTGDVVVAAVGRAEMIRG
                     SWIKPGAVVIDVGMNRNAAGTLCGDVAFDEAKEVAGLLSPVPGGVGPMTIAMLLQNTC
                     EAAIRRAAGG"
     gene            1757..2152
                     /locus_tag="VF357_09615"
     CDS             1757..2152
                     /locus_tag="VF357_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEX7524449.1"
                     /translation="MQPPPDRRYSRSHAWAMPGGQGDVRSGLTHVPGAFLGDVVSVEL
                     PPPRTEVFAGEPIGLVESTTTVFELLSPLSGIVVAVNPETESAPRKVTEDPYGEGWLL
                     SIRPAVREEFDALLTAEEYVRFAGEDQEP"
     gene            complement(2153..3091)
                     /locus_tag="VF357_09620"
     CDS             complement(2153..3091)
                     /locus_tag="VF357_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7524450.1"
                     /translation="MGLSVLLHLLVLWMAYRFPSTVRPPEEVMEVDLSDIPRATDFLP
                     AERGILEGRPPLPAPPSPARKETPVPPVLQGRVPDLPVKPDLPPEESFPVTARKASPA
                     GEANDSSGPQGKEAGGREAKAEPSPSPVTPRTAPGKSGAPGSAPPKSLRDLTPSLGKM
                     VMAREEPSGGRGQGAAAGNAVGTGGEATGKEGVTEEGGGGFRLTPLNAPEVQYISYFA
                     SIKRKIELVWQYPYEAAVAGIQGELTVDFVIARSGAVNSIELVRSSGSKILDDEAIRS
                     IRKAAPFDPIPAAYKIPGLQIRGRFVYVHGGALRLR"
     gene            3300..3584
                     /locus_tag="VF357_09625"
     CDS             3300..3584
                     /locus_tag="VF357_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003215863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7524451.1"
                     /translation="MTKAELVEVVRAEAEIGKGQAEDAVEAFLGAVTKSLKKGDKLTL
                     TGFGTFSVSNRKARTGRNPQTGEAIKIKAAKVPKFTAGKGLKEAVGGKKK"
BASE COUNT          656 a         1194 c         1257 g          580 t
ORIGIN      
        1 ggagttcgag gacctgatga agggtggaat cctcgacccg accaaggtga cccgcgttgc
       61 gctgcagaac gccgcatcgg tggcgggcct catgatcacc accgagtgcg ccatcgccga
      121 gaagcccgag gagaagaagg aaatgccgca gatgcccccg ggcggcggcg ggatgtacta
      181 gaatagagga tagcgggagc gctccccggg ccgctgcggc ccggggagct ttttttcgtt
      241 tcgcgaggca aggaggaatt ggatcatgcc catccacgag tacaaatgcc ggaagtgcga
      301 agaggtcgtc gaacggatcg aggggatgca cgacaaaccg atgaagaagt gcctctcctg
      361 cggcgggaag gtcgaccaga tgatctcctc ctccgccttc gtcctgaagg gaaccgggtg
      421 gtacgccacc gactacggga ccaagagcca cgacaacggg aacggcaagg ggaaaggaaa
      481 cgggaacgga aacgggaaag gggagccgaa ggcgaaggag gctgcggagg cgtcctgccc
      541 ggcggcatcc ggggccggag cgcccgccgc ctgcgccggc tgtcccaagc tcgactaagc
      601 gctccatttc atcaatacgg aaacatatgt acactttcac atcggatcgg gacgcagggc
      661 gcagcggaga ggaagccccc cgccccaata cgagctccgc gctgctgggg tacccccgca
      721 gtaggaataa cggggggcac gagcccggag tctgagtact caattaattg ttgccgtatt
      781 tatgaaccgc agcagtagcc ccctccctgc aggttgatcc tccgtccttg ccccttccct
      841 cgggattggt gtagataaga gagactccac tcgaccgggg gtaccgcatg accgccaagt
      901 tgatcgacgg gaaggccatc gccgcgtccg tccgggccgg cgtcaaggag cgggtggcgg
      961 agttcaccgc ccgcacgggc atccgccccg gcctcaccgt cgtcctcgtg ggggaaaacc
     1021 ccgcgtcgca ggtgtatgtc cgcaacaagg ggaaggcggc ctccgaggcg gggttcctct
     1081 cccgccagat cgacctcccc gcggtaacga aggagtccga actgctcgat ctcgtggcgc
     1141 ggctcaacgc cgacgacacc gtccacggga tcctcgtcca gctccccctt ccgggacaga
     1201 tcgacgaatc aaaggtgatc gaggcgatcg atccggccaa ggacgtcgac ggcttccatc
     1261 cggtgaacgc cggacgcctc ttcacgggcg gtacctcgtt cctcccgtgc acgccgtacg
     1321 gcatcctcac catgcttgac tacgaaaagg tggagctgaa agggaagcac gccgtcgtcg
     1381 ttgggcgcag caacatcgtc gggaagccgg tcgccatcct gctgctctcg cgccacgcga
     1441 ccgtgacgat ctgccactcg cgcaccgtcg acctcccctc cgtcgtccgg accggggacg
     1501 tggtggtggc tgcggtgggg cgggcggaga tgatccgggg ttcctggatc aagccggggg
     1561 ccgtcgtgat cgacgtcggg atgaaccgga acgcggcggg aaccctttgc ggcgacgtcg
     1621 ccttcgacga ggcgaaggag gtggcggggc tcctctcccc cgttcccggc ggggtcggcc
     1681 cgatgacgat cgcgatgctg ctgcagaaca cctgcgaagc ggcgatccgc cgcgcggcgg
     1741 gcgggtagac cccgcgatgc aaccgccgcc ggaccgccgc tattcgcggt cccacgcctg
     1801 ggcgatgccc ggcggtcaag gcgatgtgcg ctccgggctg acccacgtgc cgggcgcgtt
     1861 cctcggggac gtcgtctccg tggagcttcc tcctccgcgg acggaggtat tcgccgggga
     1921 gccgatcggg ctggtcgaat ccaccaccac cgtgttcgaa ctcctgtcgc ccctctccgg
     1981 catcgtggtc gccgtgaacc cggaaacgga gagcgccccg cggaaggtga cggaagatcc
     2041 gtacggcgag gggtggctgc tgtcgatccg cccggccgtc cgcgaagagt tcgacgccct
     2101 tctcaccgcg gaggagtacg ttcgcttcgc gggggaggat caagaaccct gatcagcgca
     2161 gccgaagcgc cccgccgtgg acgtagacga agcggccgcg gatctgcagg ccggggatct
     2221 tgtacgcggc ggggatcgga tcgaacgggg ccgccttccg gatggagcgg atcgcctcgt
     2281 cgtccaggat cttcgacccc gagctgcgga ccagttcgat cgaattcacc gctccactgc
     2341 gcgcgatcac gaaatcgacg gtgagctcgc cctggatccc ggccacagcg gcttcgtacg
     2401 ggtattgcca caccagctcg atcttccgct tgatcgacgc gaaatacgag atgtactgga
     2461 cctccggcgc gttcagcggc gtcaggcgga acccgccgcc tccctcctcg gtgacgccct
     2521 ccttcccggt cgcttcccct ccggtgccca ccgcgttccc cgccgcggct ccctgcccgc
     2581 ggcctcccga cggttcttcg cgcgccatca ccatcttgcc gagggaagga gtgaggtccc
     2641 gcagcgactt cggcggggcg ctccccgggg ctccgctttt tccgggggcg gtccgcggcg
     2701 tcacggggga aggcgacggc tccgcctttg cctcgcgccc gccggcttcc tttccctgcg
     2761 gcccggagga atcgttcgcc tccccggccg gactcgcttt ccgtgcggtg accgggaacg
     2821 actcctcggg cggcaggtcc ggcttcacgg ggaggtccgg gacgcggcct tgcagcaccg
     2881 ggggaaccgg ggtctccttc ctcgccggcg agggaggcgc ggggagcggc ggccgcccct
     2941 cgaggatccc gcgctccgcc ggcaggaagt ccgttgcccg gggaatgtcc gagaggtcga
     3001 cctccatgac ctcttccggg gggcggaccg tggaaggaaa ccggtacgcc atccagagga
     3061 cgagaaggtg caggagcacg gagaggccca tgcacgtgag gaaccggcgg cgcgatggca
     3121 taacatttga ttatagcaac tcgggtcttc attccaacgt ttccgggcga cgagacgatt
     3181 tcgccgggac ggtttaggtc ttgacacgcc ggcgtctttc gggactaatg aaaacaactt
     3241 ccctcggggt ttttcccggt ctccgggaac gtcataaaaa actttaggag gttcggcaga
     3301 tgacgaaagc ggaactggtc gaggtcgttc gcgcagaagc ggagatcggg aaagggcagg
     3361 cggaagacgc ggtcgaggcg tttctcggcg cggtcaccaa gagtttgaag aagggcgaca
     3421 aactcacgct gaccggattc gggacgttca gcgtcagcaa ccggaaagcc cgcacggggc
     3481 gcaaccccca gacgggcgag gcgatcaaga tcaaggcggc caaggttccc aagttcaccg
     3541 cgggcaaggg actcaaggag gcggtcggcg ggaagaagaa gtaaggcgcg cctgcgtccg
     3601 ttccggcagt cgcctctccg atagattttg cgccccggcc gaaccggtat ccggccgggg
     3661 cgtttccatt ccgggggtgg ccatgtt
//
LOCUS       DASVJL010000460         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_361239, whole genome shotgun sequence.
ACCESSION   DASVJL010000460 DASVJL010000000
VERSION     DASVJL010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361239"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VF357_09630"
     CDS             <1..1148
                     /locus_tag="VF357_09630"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7524452.1"
                     /translation="SADYGAVIRRSRKVADRMSRGVTYLFKKNGIELFPASASVLSPT
                     TVRVGEEELSAKNILVSSGTSVRGLPGIEPDGRVILTSDDALAQETLPRSVIVLGGGA
                     VGVEFAYVYRAFGADVAIVEMEDQLLPRTDREVAKELEKALGKQGIRVLTSAPAKGFD
                     KVTRTLTISSGGKEEALTAEKLLVAVGRKVLSDGLGLEACGVEIERGLVKVDDRLRTA
                     CPTIWAIGDVIGGMMLAHKASAEGVVAAEAIAGKEVRRPDPDRIPACVYCRPEVATIG
                     LSEEEAKRRGIEYRASKFPFAALGKAVASGHTDGFVKMIAEAKYGEVIGCHIIGAGAP
                     DMIAELSLARSLETTFHEIGRTIHAHPTLPEAIREAALMLGGEAIDL"
     gene            1168..>1805
                     /locus_tag="VF357_09635"
     CDS             1168..>1805
                     /locus_tag="VF357_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX7524453.1"
                     /translation="MTEQKDRELYRWLRLIREFEDRVSALDKQGKLMGGVYSGKGQEA
                     MTVGFCHDLRWDDWVFPLHRDLGAYLVKGADPKRLMAQIFGKRDGFAKGKDSYLHGGD
                     LANGIFGSTSMLAATLPVAAGMAYKFKIRKEDRVAIAFFGEGASSRGDVHEAMNFAGV
                     HKLPVIFVCENNFYAYSTPNELQFGVDNVADRAAGYGFKGEVVNGNDLHAVM"
BASE COUNT          316 a          553 c          660 g          276 t
ORIGIN      
        1 tttccgcgga ctacggcgcc gtcatccgcc gaagccggaa agtggcggac cggatgtccc
       61 gcggcgtgac gtacctcttc aagaagaacg ggatcgagct gtttccggcg agcgcgtcgg
      121 ttctttcgcc caccaccgtc cgggtggggg aagaggagct ctcggcgaag aacatcctcg
      181 tttcctccgg aacgtcggtg cgggggctgc cggggatcga gcccgatggg cgggtgatcc
      241 tgaccagcga cgacgccctg gcgcaggaga cgcttccgcg ctccgtgatc gtcctcgggg
      301 gaggggcggt gggggtggag ttcgcctacg tctaccgggc gttcggcgcc gacgtggcga
      361 tcgtcgagat ggaggaccag ctcctcccgc ggaccgaccg cgaagtggcg aaggagctgg
      421 agaaagcgct cgggaagcag gggatccggg tgctgacctc cgccccggcg aagggattcg
      481 acaaggtgac aaggacgttg acgatttctt ccggcgggaa agaggaggcc ctcaccgccg
      541 agaaactcct cgtcgccgtg gggcgcaagg tgctctccga cgggctcggg ctggaagcgt
      601 gcggagtcga gatcgagcgc ggtctcgtca aggtcgacga ccgtctccga accgcctgtc
      661 cgacgatctg ggccatcggc gacgtgatcg gcgggatgat gctcgcccac aaggcgtccg
      721 cggaaggcgt cgtcgcggcc gaggcgatcg cggggaagga agttcgccgc cccgacccgg
      781 accggatccc ggcgtgcgtg tactgccgtc ccgaggtggc cacgatcggc ctgtcggagg
      841 aggaggcgaa gcggcgcggg atcgaataca gggcgtcgaa gttccccttc gccgcgctcg
      901 ggaaggcggt cgcctccggg cacaccgacg ggttcgtcaa gatgatcgcg gaggcgaaat
      961 acggcgaggt gatcggctgc cacatcatcg gcgccggcgc accggacatg atcgcggagc
     1021 tctccctcgc ccgttccctc gagacgacgt tccacgagat cggccgcacc atccatgcgc
     1081 accccaccct tccggaggcg atccgggagg cggcgctcat gttgggcggc gaggcgatcg
     1141 acctataact tttcacgaga gggcgccatg accgagcaga aagaccggga gctgtaccgc
     1201 tggctccgcc tcatccgcga gttcgaggac cgggtctccg cgctggacaa gcaggggaag
     1261 ctgatgggcg gggtctactc ggggaaaggg caggaggcga tgacggtcgg cttctgccac
     1321 gacctgaggt gggacgactg ggtcttcccg ctgcaccggg acctgggcgc ctatctcgtc
     1381 aagggggccg acccgaagcg gctgatggcg cagatcttcg gcaagcggga cggattcgcg
     1441 aaggggaaag actcctacct gcacggcggg gaccttgcga acggcatctt cggctccacc
     1501 tccatgctgg cggccacctt gccggtcgcg gcggggatgg cgtacaagtt caagatccgg
     1561 aaagaggatc gcgtggcgat cgccttcttc ggcgaggggg cgagcagccg gggggacgtc
     1621 cacgaggcga tgaacttcgc cggcgtacac aagctccccg tgatcttcgt ctgcgagaac
     1681 aacttctacg cctactccac cccgaacgaa ttgcagttcg gggtcgacaa cgtggcggac
     1741 cgggccgcgg gctacggctt caagggcgag gtggtgaacg ggaacgacct gcatgcggtg
     1801 atgaa
//
LOCUS       DASVJL010000461         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1769775, whole genome shotgun sequence.
ACCESSION   DASVJL010000461 DASVJL010000000
VERSION     DASVJL010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769775"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1951
                     /gene="lon"
                     /locus_tag="VF357_09640"
     CDS             <1..1951
                     /gene="lon"
                     /locus_tag="VF357_09640"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7524454.1"
                     /translation="NPGVLADLVASNLRLKIEEAQSVLEEEDPVARLNLVSNLLSREL
                     QLAEMQAKIQNQAKEEMSKSQRAYFLRVQMKAIKQELGDIDGKSEEFDDLKGKVEAAG
                     MPEEVKKEAEKQLRRLEGMHPDAAESSVVRTYLDWMVELPWKKETKDNIRIGKAKEIL
                     DEDHHDLEKVKERILEYLSVRKLKDKMKGPILCFVGPPGVGKTSLGKSIARAMGRKFI
                     RMSLGGIRDEAEIRGHRRTYVGALPGRVIQGMKQAGTRNPVFMLDEIDKVGADFRGDP
                     SAALLEVLDPEQNFAFSDNYLNVPFDLSKVLFIATANVTDPVPPALKDRMEIIYLSGY
                     TDVDKLAIAKKYLLPRQFEENGIRPGKITMRDKAILAIIHEYTREAGLRNLEREIGQV
                     CRKLARKIAEGEKGPFAVTPKSLAKYLGIPRNLPETEGEADEVGVATGLAWTPTGGDI
                     LFVEVSLVDGKGGVMITGSLGDVMKESAQAAITYARSRAAMLGLPRNFHSKHDVHIHV
                     PSGGIPKDGPSAGITIATALVSSLTGIPVRRDVAMTGEITLRGRVLPIGGLKEKALAA
                     LRAGINHVIAPARNGKDLEEIPRHEARQVRFTLVKTMDEVLSLALTRNPFQWEKRKAK
                     PRREQSAPAEGPRKPKLAPRAKEPR"
BASE COUNT          430 a          647 c          754 g          282 t
ORIGIN      
        1 caaccccggc gtgctcgccg acctcgtggc gtcgaacctg cgcctgaaga tcgaggaggc
       61 gcaatcggtg ctcgaggagg aagaccccgt cgccaggctg aacctggtca gcaacctcct
      121 gtcccgcgag ctgcagctgg cggagatgca ggcgaagatc cagaaccagg cgaaggagga
      181 gatgtccaag agccagcggg catatttcct gcgggtgcag atgaaggcga tcaagcagga
      241 gctgggcgac atcgacggga agtccgagga gttcgacgac ctgaagggga aggtcgaggc
      301 cgccggaatg cccgaagagg tgaaaaagga ggcggagaag cagctacgcc gcctggaagg
      361 gatgcatccc gacgcggccg agtcgtccgt ggtgcgcacc tacctcgact ggatggtgga
      421 gctcccctgg aagaaggaga cgaaggacaa catccgcatc ggaaaagcga aggagatcct
      481 cgacgaggac caccatgacc tcgagaaggt caaggagcgg atcctcgaat acctgtccgt
      541 gcgcaagctg aaggacaaga tgaaggggcc gatcctctgc ttcgtcggtc ccccgggcgt
      601 ggggaaaacg tccctcggga agtcgatcgc ccgcgcgatg gggcggaagt tcatccgcat
      661 gtccctgggc ggcatccgtg acgaggcgga gatccgcggg caccgccgga cgtacgtggg
      721 ggcgcttccc gggcgcgtca tccaggggat gaagcaggcc gggacgcgca acccggtctt
      781 catgctggac gagatcgaca aggtgggcgc cgacttccgg ggcgatccgt ccgcggcgct
      841 gctcgaggtg ctggaccccg agcagaactt cgcgttcagc gacaactacc tgaacgtccc
      901 cttcgatctt tcgaaggtcc tgttcatcgc cacggcgaac gtcaccgacc ccgtcccgcc
      961 ggccctcaag gaccggatgg agatcatcta cctgtcggga tacacggacg tcgacaagct
     1021 ggcgatcgcg aagaaatacc ttttgccgcg ccagttcgag gagaacggga tccggccggg
     1081 aaagatcacg atgagggaca aggcgatcct cgccatcatc cacgaataca cgcgggaggc
     1141 ggggctgcga aacctcgagc gggagatcgg ccaggtgtgc cggaagctgg cccggaagat
     1201 cgccgaggga gagaaggggc ccttcgccgt cacgccgaag agcctcgcga agtacctcgg
     1261 gattccacgg aaccttccgg aaacggaggg ggaggccgac gaggtcgggg tggcgacggg
     1321 tctcgcctgg acgccgacgg ggggagacat cctcttcgtc gaggtgtccc tcgtcgacgg
     1381 gaagggaggc gtgatgatca ccgggtccct cggcgacgtg atgaaggaga gcgcccaggc
     1441 ggcgatcacc tacgcccgtt cgcgcgcggc gatgctcggg ctgccgcgga atttccactc
     1501 gaagcacgac gtccacatcc atgtcccgtc ggggggaatc ccgaaggacg gtccttccgc
     1561 cgggatcacg atcgcgacgg cccttgtctc ctcgctcacc gggatccccg tccgcaggga
     1621 cgtggcgatg accggcgaga tcaccctccg cggccgggtc ctcccgatcg gcggcctgaa
     1681 ggagaaggcg ctggcggcgc ttcgcgccgg gatcaaccat gtgatcgccc cggcgcggaa
     1741 cgggaaagac ctcgaggaaa tccctcgcca cgaggcccgg caggtgcgct tcacgctggt
     1801 gaagacgatg gacgaggtcc tctccctcgc cctgacgagg aacccgttcc agtgggaaaa
     1861 gaggaaggcg aagccccgcc gggaacagag cgcgccggcg gaggggccga ggaagccgaa
     1921 gctggcgcct cgcgcgaaag agccgcggtg aagcggacct ccagggacgc gtccgacgga
     1981 accgggcccg cgcccgcatt tctcaccagg gttcgtagcg aggtggtgga gacgggcgtg
     2041 gatacaaggc gcgcgaccga gggccccgga ggcgtagttg agactacgtc gaggagcccg
     2101 accgagcgca acg
//
LOCUS       DASVJL010000462         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_736154, whole genome shotgun sequence.
ACCESSION   DASVJL010000462 DASVJL010000000
VERSION     DASVJL010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736154"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="VF357_09645"
     CDS             <1..1208
                     /locus_tag="VF357_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7524455.1"
                     /translation="STGSGNTGFAAMPDRAPGGIAQGVLEDLRPIFTTKMVLSAWIPC
                     FLITVLHYGTGAHHAWGHDVLRRLYYLPILFAAFAGGLRGGVTVSVFASLVYLPHAFT
                     SLLVQDPGDALEKGLEIILYNIVAVVAGLLVDREHRERQQQERLATRLGDALAEQRRI
                     EAQLIRAGKLGALGEMTAGIAHEIKNPLHALKGTAEILRDAVPEGVPERRMLELHIEE
                     IDRLGQTADRFLSFARPIPADRRPVEPKSLIDRVVSLVSTQARKEGVETVVATNGAVD
                     LPKAMGDPDLLAQLLLNIALNGVQAMVPGGGGTLAFSLGTERQGGKEYVRVRVANTGP
                     PIPEKNLERIFDPFYTTKDGGTGLGLSICSRIADEHEGTLAVRNLPGGGGVEFSLFLP
                     AMEGIDARR"
     gene            1195..>2115
                     /locus_tag="VF357_09650"
     CDS             1195..>2115
                     /locus_tag="VF357_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX7524456.1"
                     /translation="MPDDNSLSEVHSSVAVPESRGFLRRLFAFAGPAYLVSVGYMDPG
                     NWATDIAAGSRYGYALIWVLLMSNLMAVLLQSLSARLGLVSGRDLAQACRDRYPPAVN
                     AALWVLCEVAIGACDLAEVIGSAIGLQLLFGLPLMYGVLITALDTFLILFLHQAGIRK
                     MEAFIIVLVGTIGSCFLLEIFLSRPDLPGIARGFLPSLPDRDALYYAIGILGATVMPH
                     NLYLHSSLVQSRRVVKTANGIHRSLKYNTIDSVVALNIAFLINAAILVMAAAVFFRSG
                     HTDVASIQDAHALLAPLVGSRVAPALFAVAL"
BASE COUNT          306 a          699 c          741 g          369 t
ORIGIN      
        1 cgtctaccgg atcgggaaat acgggcttcg ccgcgatgcc tgaccgcgcg cctgggggaa
       61 tcgcacaagg ggtgctggag gacctgcggc cgatcttcac gacgaagatg gttctctccg
      121 cgtggatccc atgttttttg atcaccgtcc tgcactacgg cacgggggcg caccacgcgt
      181 gggggcacga cgtcctccgc cggctctact acctgccgat cctgttcgcg gcgttcgcgg
      241 gcggcttgcg gggcggggtc accgtttccg tgttcgcctc cctggtctac ctcccgcacg
      301 ccttcacgag cctcctggtg caggacccgg gggacgcgct ggagaagggc ctggagatca
      361 tcctctataa catcgtggcg gtcgtggccg gcctgctggt cgaccgggag caccgcgagc
      421 ggcaacaaca ggagcggctt gcgacccggc tgggagacgc cctcgcggag cagcggcgga
      481 tcgaagcgca gctgatccgc gccgggaaac tgggggcgct gggcgagatg acggcgggga
      541 tcgcccacga gatcaagaac ccgctccacg ccctgaaggg gaccgcggag atcctgcgcg
      601 acgccgtccc ggaaggggtg cccgagcggc ggatgctcga gctgcacatc gaggagatcg
      661 accggctcgg ccagaccgcg gaccggttcc tctccttcgc gcggccgatc cccgcggatc
      721 gtaggccggt ggagccaaaa tcgttgatcg accgcgtggt atcgctcgtg tcgacccagg
      781 cccgcaaaga gggggtggag acggtcgttg cgacgaacgg ggccgtggat ctcccgaagg
      841 cgatggggga tccggacctg ctggcgcaac tcctgctgaa catcgcgctg aatggggtgc
      901 aggcgatggt cccggggggt ggcggaacgt tggccttctc cctcgggacg gagcggcagg
      961 gagggaagga gtacgtgcgg gtgcgcgtcg cgaacacggg gccgccgatc ccggagaaga
     1021 acctcgagcg gatcttcgac ccgttctaca ccacgaagga cggcgggacg gggctcgggc
     1081 tctccatctg ctcccgcatc gccgacgagc acgaggggac cctcgcggtt cggaaccttc
     1141 cgggaggagg gggcgtggag ttttccctct tcttgccggc gatggagggg atcgatgccc
     1201 gacgataact ccctctccga agtccactcg tcggtggcgg tcccggaatc gcgcggattc
     1261 ctccggcgtc tcttcgcgtt cgccggaccc gcgtacctcg tgtcggtcgg gtacatggac
     1321 ccggggaact gggccaccga catcgccgcc gggtcgcggt acgggtacgc cctgatctgg
     1381 gtcctcctga tgtcgaacct gatggccgtg ctgctgcagt ccctctcggc gcggctggga
     1441 ctggtctccg gcagggacct cgcgcaggcg tgcagggacc ggtatcctcc cgcggtgaac
     1501 gcggcgctgt gggtcctatg cgaggtggcg atcggggcgt gcgacctcgc cgaggtgatc
     1561 gggtcggcga tcgggctgca gctgctcttc ggcctcccgc tgatgtacgg ggtcctcatc
     1621 accgcgctgg acacgttcct catcctgttc ctccaccagg cggggatccg gaagatggag
     1681 gcgttcatca tcgtcctcgt ggggaccatc gggagctgct tcctgctcga gatcttcctc
     1741 tcccgcccgg accttccggg gatcgcgcgc gggtttctgc cgtcgttgcc ggaccgggac
     1801 gccctctact acgcgatcgg catcctcggg gcgaccgtga tgccgcacaa cctctacctt
     1861 cattcctccc tagtccagtc gcgcagggtg gtgaagaccg cgaacgggat ccaccggtcg
     1921 ctgaagtaca acacgatcga ttcggtggtg gccctcaaca tcgcgttcct catcaacgcg
     1981 gcgatcctgg tgatggcggc ggcggtcttt ttccggtcgg gccacacgga cgtcgcgtcg
     2041 atccaggacg cccacgcgct cctcgccccc ctcgtgggga gcagggtcgc cccggccctc
     2101 ttcgccgttg ccctg
//
LOCUS       DASVJL010000463         5565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1769975, whole genome shotgun sequence.
ACCESSION   DASVJL010000463 DASVJL010000000
VERSION     DASVJL010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769975"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1425)
                     /locus_tag="VF357_09655"
     CDS             complement(73..1425)
                     /locus_tag="VF357_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX7524457.1"
                     /translation="MTPVRARASIPPEWLSSLRNLIESASGVVLGDAKLPHLSAVVRG
                     RMAILGIPDAPGYMEFLSEGERGAAERRDLVLELLVGETSFFRTPVLFQAFQKAILPE
                     LLEQEFVDPLSIWCAGCATGEEAYSVAIAVLEGSGGRGADPVRIRATDLHPRFIDAAR
                     EGIYPAEALRNLPGRIKESWFAPMKDGRYRVTDAVRRLVDFEERNLIDFLSDPLPSAR
                     YAAIFCRNVMIYFRSDTTRRLVERFHESLLDGGVLFLGHSETLWGISEAFRLEQREKI
                     FYYRKPRAESVPPRPAAAPRSSGSSPPRRAIPRAEPPPPVDESSSAMKLVLSAERMAD
                     EGRFEDAVAMCRQAAALDPECPEPEYLMAFLLRGNGRHGEALYHAERALERDSQFVFA
                     DMEAAECLCGMGRAGEATLRWKGILRAIEGNVRLPRLSVGAGISPQMLRQYVLSRLPR
                     "
     gene            complement(1425..2885)
                     /locus_tag="VF357_09660"
     CDS             complement(1425..2885)
                     /locus_tag="VF357_09660"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7524458.1"
                     /translation="MFRVLLVDDSETVTGMLSMVLGSAGYAIETAGDGVKGVRAAFRR
                     VPDLVVMSARMPRLDGIQACRLLKAEPATRDVPVILLTSEEAGVERLHAARAGGDRCL
                     LHVSPEEVLPVVRECLAGKAPRPEGGANPGVAPPSDFEILSRVNGVLEATLFEASLLN
                     EIARVGREVEDFEATARELFRLLREIAPAEAMGAVFSDGVYSEGVSVFPDEAGDPFRA
                     EVRQRTERLRDEVGVPFAPDRAVWTGIQGGFLGSDGIAPESLVTTTVCSVRTGEMVKG
                     LIAVYTGMEGSALGSASTKEILLRQAFIVLENAWLYRQIARISVTDGLTGLMNVRHFR
                     EQAQREHSLARRHNDPYSVLMMDIDHFKKINDVYGHPVGDIVLREIAAIFRKAVRLTD
                     LPARYGGEEFVVFLPRTRLPESVTVGERIRETVERMVFAAPSPMLRCTVSVGIAEYLP
                     GMEGTEKTVIGRADQALYSAKHGGRNRVVYTSAKET"
     gene            complement(2887..3981)
                     /gene="cheB"
                     /locus_tag="VF357_09665"
     CDS             complement(2887..3981)
                     /gene="cheB"
                     /locus_tag="VF357_09665"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HEX7524459.1"
                     /translation="MTSSPASGDPVRILVVDDSPSIRAVIRAMVEGDKGIRIVGEAGT
                     GGEAVEMARTLRPGVILMDIQMPEMDGIEATERIMASEAVPIIAFSAFTWGGEAKASI
                     EMLAAGALDVMAKPDLGGAGAVSECSRVLRKKIRSASRVAVVRHLRRAGPRIQEGGEP
                     AGPSDRTRFEVLGVGASTGGPTALRDLFSALPADFPMPILVVQHITMGFTAGFVEWLR
                     QHTSLDVRVANEEDRAVPGSILIAPEGRQLEALPGGAVRTTSRKPRGVHLPSADTLLS
                     SLASAYGRKCIGALLTGMGSDGAEGLLDIRRAGGLTLAQDEETCVVFGMPREAVRRGA
                     VDRSMAPASMAELLRQMTEPSARRRGGGRA"
     gene            complement(3978..>5565)
                     /locus_tag="VF357_09670"
     CDS             complement(3978..>5565)
                     /locus_tag="VF357_09670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7524460.1"
                     /translation="GLAQGEFSPPFARRVAPFVEEGKAVFQEVGTQRSALKRRESVVA
                     TTLSQNLEELRLEVMAIRTVPLLPLFDSFHPMVGALARELGKDVEVLVRGGKTEIDRK
                     VAEALAEPLTHILRNAVDHGIETSAERERAGKPRKGRIVITATPMKGRVVLEVEDDGR
                     GIDPNEVREAAIRKGMISEKAAWRLDDRELIDFVFRTGFSTAKTMTGFSGLGIGMDVV
                     HATAERFNGTSEIHSNPGKGTRVVMELPFSMAVSRVLLFLSGDQYFALPIMHADGIHP
                     FAGRDIVTLEGRKSLRLGETPVPLAWLNRLLGLPDARDPGDRYLAVMVRQSGKRIALV
                     IDQVEGECEVVVRDLGKYLGKVPLFMGSTILGTGEVALLLDVYDLMTAVRLHAETSPE
                     GVGEGVRPGVDADILVVDDSLLVREMQQRILFSSGYRVETASGGKAALERLSHKRYHV
                     VVVGVRMAGMDGIQLLSEARKTDYTRDLPFILVATKEHREDLVRARAAGAKGCVTREE
                     FTPDRMAAMIGSILGRGAGG"
BASE COUNT          937 a         2026 c         1763 g          839 t
ORIGIN      
        1 agtggggaca atggtaatcg gtccccgcca tccggagatt atctcagcgg gaaggggccc
       61 ccgcaacggt attcatcgcg gcaatcggga gagcacgtat tggcgcagca tctgcggaga
      121 gattcccgcc cccaccgaaa gacgcgggag gcgcacgttc ccctcgatgg cgcgcaggat
      181 ccccttccac cgcagcgtcg cctccccggc ccgccccatc ccgcagaggc actccgccgc
      241 ctccatatcc gcaaagacga actgcgagtc ccgttcgaga gcgcgctcgg cgtggtagag
      301 cgcctccccg tggcgtccat ttcctcgaag aaggaacgcc atcaggtatt ccggctcggg
      361 gcactccgga tcgagggccg ccgcctggcg gcacatcgcg accgcatcct cgaagcgccc
      421 ctcgtcggcc atccgctccg ccgaaaggac cagtttcatc gccgaagagg attcgtccac
      481 gggaggagga ggctccgcgc gcgggatcgc ccgtctcggg gggctcgatc cggaggaacg
      541 gggcgcggcg gcaggacgcg gcgggacgct ctccgcgcgc ggtttccggt agtagaaaat
      601 cttctccctc tgctccagcc ggaacgcctc cgaaatcccc cacaacgttt cggagtgtcc
      661 gaggaagaga accccgccgt cgagaagact ttcgtgaaac cgctcgacga gccgccgcgt
      721 ggtgtccgac cggaagtaga tcatcacgtt ccggcagaag atcgccgcgt accttgcgga
      781 ggggagggga tcggagagaa aatcgatcag gttccgctcc tcgaagtcga ccagccgccg
      841 caccgcatcc gtcacccggt accgcccgtc cttcatcggc gcgaaccagc tctccttgat
      901 ccggcccgga aggttccgca acgcctccgc gggatagatc ccttccctgg ccgcatcgat
      961 gaaccgcggg tggaggtccg tggcgcggat ccggaccggg tcggcgcccc tcccgccgga
     1021 tccctccaag acggcgatgg cgacggagta cgcctcctcg cccgtggcac accccgcgca
     1081 ccagatggaa agcgggtcca cgaactcctg ctcgagcagc tccgggagaa tcgccttctg
     1141 gaacgcctgg aagagaaccg gggtccggaa aaagctcgtt tccccaacga ggagttccaa
     1201 caccaggtcg cgccgctccg ccgcccccct ctctccttcg gagagaaact ccatgtaccc
     1261 cggcgcgtcg gggatcccga gaatcgccat cctgccccgg acgacggcgg acagatgcgg
     1321 aagcttggcg tcgccaagga cgaccccgct ggcggattcg atcaggttcc gcagggagga
     1381 gagccattcg ggggggatgg aggcccgcgc gcgcaccggc gtcatcacgt ctctttcgcg
     1441 gaggtgtaca ccacgcggtt ccgtcctccg tgcttggcgg aataaagcgc ctgatcggcg
     1501 cgcccgatca cggttttctc cgtcccctcc atccccggga ggtactccgc gataccgacg
     1561 ctcacggtgc agcggagcat cggcgacggc gcggcgaaca ccatccgctc caccgtctcc
     1621 cggatccgtt ctcccacggt gaccgactcg ggaagccggg tccgggggag gaagacgacg
     1681 aactcctccc cgccgtaccg ggcgggaagg tcggtgaggc ggaccgcctt ccggaaaatc
     1741 gcggcaattt cccgcaggac gatatctccc acgggatggc cgtagacgtc gttgatcttc
     1801 ttgaaatggt cgatatccat catcaggacg gagtacggat cgttgtggcg gcgtgcgagg
     1861 gagtgctccc gctgcgcctg ttcccggaag tggcggacgt tcatgagccc cgtcagcccg
     1921 tccgtcacgg agatccgcgc gatctgccgg tacagccagg cgttttcgag gacgatgaac
     1981 gcctgccgga ggaggatctc cttcgtcgac gcgctgccca gggccgatcc ttccatcccc
     2041 gtgtacaccg caataagccc tttgaccatc tcccccgtgc ggaccgagca gacggtcgtt
     2101 gtcacaagcg attccggcgc gatgccgtcg gacccgagga atcccccctg gatgccggtc
     2161 cagacagcgc ggtccggggc gaacggaaca ccgacctcgt cgcggagccg ctccgtcctc
     2221 tggcggacct ccgcccggaa cggatccccc gcttcgtcgg ggaagacgga gacgccctcc
     2281 gagtacacgc cgtcggagaa gaccgccccc atcgcctcgg cgggagcgat ctcccggagc
     2341 agtcggaaca actccctggc cgtcgcttcg aaatcttcca cctcgcggcc cacgcgcgcg
     2401 atctcgttca acaagctcgc ctcgaacagg gtcgcctcga gaacgccgtt gacccgggaa
     2461 aggatttcga agtcgctcgg aggcgcgacc cccggattgg ctccgccctc cggccgcggc
     2521 gcctttccgg cgagacactc gcggaccacg ggcaaaacct cctccgggga gacgtgcaac
     2581 aggcaacggt ccccgcccgc gcgggccgcg tgaagccgct ccacccccgc ttcctccgac
     2641 gtgagcagga tcaccgggac atcccgtgtc gcgggctcgg ccttgagcag gcggcacgcc
     2701 tggatgccgt ccagccgggg catccgggcc gacatcacga cgaggtccgg gacgcgccgg
     2761 aaagcggccc gcaccccctt caccccgtcg ccggccgttt cgatcgcgta ccccgccgag
     2821 ccgaggacca tggagagcat cccggtgacg gtctcgctgt cgtccacaag gagaacgcgg
     2881 aacatgtcag gcacgaccgc ccccccttcg gcgggcggac ggttcggtca tctgccgcag
     2941 cagttctgcc atggacgccg gcgccatcga ccggtcgacg gcgccgcggc gaacagcctc
     3001 gcggggcatg ccgaagacga cgcacgtctc ctcgtcctgc gcaagggtga gccctccagc
     3061 acgccggatg tcaaggagcc cctcggcgcc gtccgatccc atcccggtga gaagagcgcc
     3121 gatgcacttc cgcccgtaag cggaggccag ggaggacagg agcgtgtccg ccgacgggag
     3181 gtgcactcca cgcggtttcc gggaggttgt ccggaccgcg ccgccgggca gcgcctcgag
     3241 ctgccgtcct tccggggcga tcaggatcga ccccggcacg gcgcgatcct cctcgttcgc
     3301 aacacggacg tcgagagagg tgtgctgccg gagccactca acgaacccgg cggtgaaccc
     3361 catggtgatg tgctgcacga cgaggatcgg catagggaag tcggccggca gcgcggagaa
     3421 aaggtcgcgc agcgccgtcg ggccgcccgt cgatgccccg acgcccagca cctcgaagcg
     3481 cgtccggtcg gaaggacccg cgggttcgcc cccttcctgg atcctcggac cggccctgcg
     3541 cagatgccgc acgaccgcca cgcgggaagc ggaccggatc ttcttccgca gcacgcggga
     3601 gcattcgctc accgcccccg ccccgccgag gtccggcttc gccatcacgt cgagggctcc
     3661 ggcggccagc atctcgatgg aggccttcgc ctcccctccc caggtgaacg ccgaaaaggc
     3721 gatgatgggg acggcctccg aagccatgat cctctcggtc gcctcgatcc cgtccatctc
     3781 aggcatctgg atatccatca ggatcacgcc ggggcggagc gtccgcgcca tctccacggc
     3841 ttccccgccg gttcccgcct cgccgacgat ccggatcccc ttgtccccct cgaccatcgc
     3901 ccggatgacg gcacggatgg aaggggagtc atcgacgacc agaatgcgga ccgggtctcc
     3961 cgatgccggg gaagacgtca tccgccggcg ccccgcccga ggatgctccc gatcatcgcc
     4021 gccatccggt cgggagtgaa ctcctcccgt gtcacgcacc ccttcgcgcc cgccgccctc
     4081 gcccgcacca gatcctcccg gtgctccttc gttgcgacga gaataaaggg gaggtcccgc
     4141 gtgtagtcgg tctttcgcgc ctccgaaagc agctggatcc cgtccatccc cgccatccgg
     4201 acgccgacga cgacgacgtg gtatcgcttg tgcgaaagcc gctcgagggc cgccttcccg
     4261 ccggacgcgg tctcaacccg gtagccggag gagaagagga tccgctgctg catctcgcga
     4321 acgagaaggg aatcgtccac gacgaggatg tcggcgtcga ccccgggccg taccccctcc
     4381 cccactccct cgggggacgt ttccgcgtgc aggcgcaccg cggtcatcag atcgtagacg
     4441 tccagcagaa gcgcgacctc gccggttccg aggatggtcg atcccatgaa aagggggacc
     4501 ttcccgaggt acttcccgag gtcccggacg acgacttcgc actcgccctc gacctggtcg
     4561 atcaccaggg cgatccgctt cccggactgc cggaccatca ccgcgaggta ccgatccccg
     4621 gggtcgcggg catccggaag accgaggagc cggttcaacc aggcgagcgg gaccggggtc
     4681 tcgcccaggc gaagggactt ccgcccctcc aacgtgacga tgtcgcgtcc cgcgaatggg
     4741 tggatcccgt cggcgtgcat gatagggagc gcaaaatatt gatcgccgga gaggaagaga
     4801 aggacccggg agaccgccat gctgaacggg agctccatga ccacccgggt ccccttgccc
     4861 ggattggagt gaatctccga ggtcccgttg aaccgctcgg ccgtggcgtg gaccacgtcc
     4921 attccgatcc cgagaccgga gaacccggtc atcgtcttcg cggtgctgaa gccggtgcgg
     4981 aacacgaagt cgatcagttc ccgatcgtcc agccgccacg cggccttctc gctgatcatc
     5041 cccttgcgga tcgccgcctc gcgaacctcg ttcgggtcga tcccgcggcc gtcgtcctcc
     5101 acctcgagga cgacccgccc cttcatcggc gtcgcggtga tcacgatccg ccctttccgt
     5161 ggcttccccg cccgctcccg ctccgcggag gtctcgatcc cgtggtcgac cgcattccgc
     5221 aggatgtggg tcagcggttc cgcgagggcc tcggccacct tccggtcgat ctcggtcttg
     5281 ccgccgcgga ccagcacctc cacgtccttc ccgagctccc tcgccagcgc ccccaccatg
     5341 ggatggaacg agtcgaacaa cggcaggagt ggaaccgtcc ggatggccat gacctccaac
     5401 cggagttcct cgaggttctg cgaaagcgtc gtggccacga cgctttcgcg gcgtttcagt
     5461 gccgaccgct gggtcccgac ctcctggaac accgccttcc cctcctcgac gaagggggcg
     5521 accctccggg caaacggcgg ggagaactcg ccctgggcga ggccg
//
LOCUS       DASVJL010000464         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1301292, whole genome shotgun sequence.
ACCESSION   DASVJL010000464 DASVJL010000000
VERSION     DASVJL010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301292"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VF357_09675"
     CDS             complement(<1..437)
                     /locus_tag="VF357_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524461.1"
                     /translation="MTFAPARAVPSRGPAAVAGTALLLWLLFLPAPGDCTEEFSRRTG
                     KECAACHVDPSGGGELTVSGKAFPASIPPAGKSVLASPSRRAVRFAAGFLHLITAVMW
                     FGTILYVHLLLKPAYAAHGLPRGELLVGWGSIIVMAVTGTVLTV"
     gene            complement(434..1441)
                     /locus_tag="VF357_09680"
     CDS             complement(434..1441)
                     /locus_tag="VF357_09680"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7524462.1"
                     /translation="MRYLILGGGPAGIAAARTLRKAKSDAEIVIATDETEAPYLRPLL
                     PDLILGDVDIETVLDPQGKDLREKGIKTLAGRIATRLDTGNRRVDFSDGTKEGYDVLL
                     IATGGRPEVPPALKKGYPAIRVFDSLRDTLAIKERAALPGGAVVYGPGYLAIEACRAL
                     RKAKKEVAWIQPDLPKSGYPIAGELEASILDDVRNRGARIKDGDDIVDVREKDADTCV
                     VLTRDGEKIPCSLLVVATERVPSVGFLAGSGVTVGRGVLVDDALRTSAPNVFAAGDCA
                     EFAEKPMKVGRINFGWRSAIKQGQLAGENMAGGNRRFGSEQENYFWALFGFSLLDRAR
                     K"
     gene            complement(1486..1683)
                     /locus_tag="VF357_09685"
     CDS             complement(1486..1683)
                     /locus_tag="VF357_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524463.1"
                     /translation="MSRKHVAISVVVILLLAGVVGFLLLRGVFLKGVGVRVLWPRMAA
                     LLLLGAATLAIATRRSRKTLT"
BASE COUNT          285 a          646 c          558 g          274 t
ORIGIN      
        1 accgtcagga ccgtccccgt gaccgccatc acgatgatgg agccccaccc gaccagcagc
       61 tccccccggg gcagcccgtg ggcggcgtac gcgggtttca gcagcaggtg cacgtagagg
      121 atggtgccga accacatcac cgccgtgatg aggtggagga atccggcggc gaaacgaacg
      181 gcgcggcgcg acggggaggc aaggacgctc ttccccgcgg gcggaatcga ggcggggaat
      241 gcttttccgg aaacggtgag ctctccgcct cccgacgggt cgacgtggca ggcggcgcac
      301 tctttcccgg tgcgccggga aaactcctcg gtgcagtccc ccggtgccgg caggaacagc
      361 agccagagga gcagggcggt cccggcgacg gccgccggtc cccgggaggg gaccgcgcgc
      421 gccggcgcga aggtcacttc ctcgcccggt ccagcaggga aaacccgaag agggcccaga
      481 agtagttctc ctgttcgctg ccgaaccgcc ggtttccccc cgccatgttc tcccccgcca
      541 gctgcccctg cttgatggcg ctgcgccagc cgaagttgat gcggccgacc ttcatcggct
      601 tttcggcaaa ctccgcgcag tcacccgccg cgaacacgtt cggtgcgctc gtccggagcg
      661 catcgtccac cagcacccct ctcccaaccg tcacgccgct gcccgcgagg aaccccacgg
      721 acgggactcg ctcggtggcg accacaagga gggagcaggg gatcttctct ccgtcccggg
      781 tgaggaccac gcaggtgtcg gcgtccttct cccgcacgtc gacgatgtcg tccccgtcct
      841 tgatcctcgc tccccggttg cggacatcgt cgaggatgct cgcctccagt tcccctgcga
      901 tcgggtagcc gctcttaggc aggtccggct ggatccaggc gacctccttc ttcgccttcc
      961 gcaaggcgcg gcacgcctcg atcgcgaggt acccgggacc atacaccacg gccccccccg
     1021 ggagggccgc ccgctccttg atcgcgagcg tgtcgcgcaa ggagtcgaag acgcggatcg
     1081 ccggatagcc cttcttcagc gccggcggaa cttccggcct gccgccggtc gcgatcagga
     1141 ggacgtcgta cccctccttt gtcccgtccg aaaaatccac ccgacggttc cccgtgtcca
     1201 agcgcgttgc gatccggccc gcgagggtct tgatcccctt ctcccggaga tccttcccct
     1261 gcgggtcgag gaccgtttcg atatccacgt ccccgaggat caggtcgggg agcagcggcc
     1321 gcaggtacgg ggcctccgtt tcatccgtgg caatgacgat ctccgcgtcg gacttcgcct
     1381 tccggagggt cctggccgcg gcgattcccg ccggcccgcc tccaaggatc aggtaccgca
     1441 tgggacacct cctgtctccg catcaatgga tgtcaatagg atacctcagg tcagcgtctt
     1501 ccgggaccgc cgggtcgcga tcgccagggt cgcggcgccc aggagcagga gcgccgccat
     1561 ccggggccac agcacccgga ccccgacccc tttgaggaag accccccgga ggagaagaaa
     1621 ccccacgacc ccggcgagca ggagaatgac gacgacggag atcgcgacat gcttcctgct
     1681 catggtcggt cccccggacg tttatgcgcg acgtttcaga tccaccgctt ccgccggaag
     1741 tagtacagca tcccgcccgc gac
//
LOCUS       DASVJL010000465         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_363440, whole genome shotgun sequence.
ACCESSION   DASVJL010000465 DASVJL010000000
VERSION     DASVJL010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363440"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1107)
                     /locus_tag="VF357_09690"
     CDS             complement(46..1107)
                     /locus_tag="VF357_09690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7524464.1"
                     /translation="MAGSDRLEVVDPTRIPGWDERLARHPEATVFHTVAWARVLSETY
                     RYKPKYFTAVEEDRLVALLPFMEIRSWLTGTRGVSLPFTDECRPILPAGMPFDEAARE
                     VISRAGSRRWKTIEYRGRCRGMGRLPASSEYLTHELDLTPGEAALFSGYRSNVRRNIR
                     KAEKGGVTVASDPTPGGVREFYRLNCLTRREHGLPPQPARFFENLRSHILEKGFGTLL
                     LARHEGRAVAGAVFLHFAGKAIYKYGASDRRYQEMRPNNRVFREGIRELCGKGVRTLS
                     FGRTDPHHEGLRQFKLSWGAVERTLQYAKYDVTSKGYLSTKKIPCDDPLGAYGVEASI
                     AGPALDRTSRLPARRVVGS"
     gene            complement(1114..>2210)
                     /locus_tag="VF357_09695"
     CDS             complement(1114..>2210)
                     /locus_tag="VF357_09695"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX7524465.1"
                     /translation="LKSQYESIRPEIAEAIQQVLEKTAFAGGPFVAQLEKEFAAFCGT
                     QYAAGVGSGTEALWLALLALRVGPGDEVITVPDTFIATAEAISYCGATPVFVDVEPVT
                     FNMDPAKLEAAITKKTKAVIPVHLFGQMADMAPILAIARKHKLFVVEDASQSHGAEYN
                     GKKAGSMGDVGCFSFYPGKNLGAYGEAGAVVTNDAELDRKIRMLRDHGQEKKYHHGRI
                     GWNARMDGIQGAVLSVKLTRLPAWNEARRKNAALYDGLLSEIKGVAIPGQASYAKPVY
                     HIYAIRVKDRDAVIAALADKDIHCGIHYPIPLHMQEAYKSLGYRQGSFPVAEKAASEF
                     VSLPMYAELTKEQIARVAAELKAIVLAPVS"
BASE COUNT          353 a          756 c          685 g          416 t
ORIGIN      
        1 tttgacctga tagtaggcgt ccctaattac tggcatgata ttcagttaag agccgactac
       61 ccgacgtgcc ggtaggcgac tcgtccgatc aagcgcagga ccggcaatgg aagcttcgac
      121 accgtacgct cccaggggat cgtcgcacgg tattttcttt gttgataagt accccttcga
      181 cgtcacgtcg tatttggcgt attgcagcgt tctttccacc gccccccagg agagcttgaa
      241 ctgccgcaac ccctcgtggt gcgggtccgt gcggccgaag gagagcgtcc gcaccccttt
      301 cccgcaaagc tcccggatcc cttcccggaa gacccggttg ttcggccgca tctcctgata
      361 gcgccggtcc gaggcgccgt acttgtagat cgccttcccg gcgaaatgga ggaagaccgc
      421 tccggcgacc gccctcccct cgtggcgggc cagcaggagg gtcccgaacc ccttttccag
      481 gatgtgcgac cggaggttct cgaaaaaccg ggccggctgc ggcggcaagc cgtgctcccg
      541 gcgggtgagg cagttcagcc ggtagaactc ccgtacccca cccggcgtcg gatcggacgc
      601 cacggtgacc ccgcccttct ccgccttgcg gatgtttctc cggacgttcg agcggtaccc
      661 ggagaaaaga gccgcttccc cgggggtcag gtccagttcg tgggtcaggt attccgacga
      721 tgcgggaagg cgccccatgc cgcggcagcg cccgcggtac tcgatcgtct tccaccggcg
      781 ggacccggcc cgggaaatca cttcgcgggc cgcctcgtcg aacggcatcc cggcgggcag
      841 gatggggcgg cactcgtcgg tgaacggaag ggagaccccc cgcgtcccgg tgagccagct
      901 ccggatttcc atgaacggga ggagggcgac gagccgatcc tcctccaccg cggtgaaata
      961 cttcggcttg taccgatacg tttcggagag gacgcgtgcc caggcgacgg tgtgaaagac
     1021 ggtcgcctcc gggtgacggg ccagccgctc atcccaaccc ggaattcggg ttggatcgac
     1081 gacctccagg cggtccgatc cggccaatac ttttcagctt accggggcca ggacgatcgc
     1141 tttcaactcc gcggccaccc gggcgatctg ctccttcgtc agttccgcgt acatcggcag
     1201 ggagacgaac tccgaggcgg ctttttccgc caccgggaac gatccctggc gatagcctaa
     1261 agatttgtac gcctcctgca tgtggagggg aatcgggtag tggatgccgc aatggatgtc
     1321 cttgtcggcg agggcggcga tcacggcgtc ccggtctttc acgcggatcg cgtagatgtg
     1381 gtagacgggt ttggcgtacg acgcctgccc gggaatcgcg acccccttga tctcgctcaa
     1441 gaggccgtcg tagagggcgg cgtttttgcg gcgcgcctcg ttccacgccg gcagacgcgt
     1501 aagcttcacg cttaataccg ctccctggat cccgtccatc cgcgcgttcc agccgatcct
     1561 cccgtggtga tacttcttct cctgcccgtg gtcccgcagc atgcggattt tcctgtccag
     1621 ctccgcgtcg ttcgtgacga ccgccccggc ctcgccgtac gcccccaggt tcttccccgg
     1681 atagaagctg aagcacccca cgtcccccat cgacccggcc ttcttcccgt tgtactccgc
     1741 cccgtgcgac tgggaggcgt cctcgaccac gaacagcttg tgcttccggg cgatcgcgag
     1801 gatcggggcc atgtcggcca tctggccgaa gaggtggacg gggatcacgg ccttcgtctt
     1861 cttcgtgatc gcggcttcga gcttcgccgg gtccatgttg aaggtgaccg gctccacgtc
     1921 gacgaagacc ggtgtcgccc cgcagtagct gatcgcctcc gccgtggcga tgaacgtgtc
     1981 ggggacggtg atcacctcgt ctcccgggcc cacccgcagg gccagcaggg ccagccagag
     2041 ggcctcggtc ccgctcccca ccccggcggc gtactgcgtg ccgcaaaacg ccgcgaactc
     2101 cttctccaac tgcgccacga aggggccgcc cgcaaacgcc gtcttttcga gcacctgttg
     2161 gatcgcctcc gcgatctccg gccggatcga ctcgtactgg gacttcaagt
//
LOCUS       DASVJL010000466         3069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_176589, whole genome shotgun sequence.
ACCESSION   DASVJL010000466 DASVJL010000000
VERSION     DASVJL010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176589"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..1964)
                     /gene="rny"
                     /locus_tag="VF357_09700"
     CDS             complement(387..1964)
                     /gene="rny"
                     /locus_tag="VF357_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX7524466.1"
                     /translation="MSTVLIAVLAALAVGLGVYAAFLVSGKKSTLDKARADARAEAGK
                     AAEILQSSKKEATNILKEAALQAKDHLLQVKIDFEKETRDRKNELSQLEKRLLQKEDQ
                     LDRKNEQADARAAEYTKKEREIAEQDRKLEAAQADLDARLAASRVELERVAGLSAEQA
                     KAEIVELMADEAKMEAGKRIRVMDDEFKEEATQKARKMIALAVQRYAADYVAEHVVSA
                     VPLPSEEMKGRIIGREGRNIRAFEAATGIDVIIDDTPEAVILSGFNPVRREIARISLT
                     RLLQDGRIHPARIEETVEKVAKEIEETIREAGEQALFDLGIHGVHADLVKLIGRLKYR
                     TSYGQNIYTHSLEVAFLCGMIASELGLNAKVAKRAGLLHDIGKAVDHEVEGPHALIGA
                     DLARKYGESAKIVHAIGAHHEDESPKDILPILVQAADALSGARPGARREMLANYLKRL
                     EDLEAIAKSFAGVEKSYAIQAGREIRIIVDYQKIGDDAATLLARDIAKKIETDLSYPG
                     QIRVTVIRETRAIEYAR"
     gene            complement(1994..2599)
                     /locus_tag="VF357_09705"
     CDS             complement(1994..2599)
                     /locus_tag="VF357_09705"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX7524467.1"
                     /translation="MLVLERKEILRRESRIRPRERAESAVSAEAGDRAQARFLREFPP
                     RAGMAAALYCALAGEVPTERIRHAYLAAGARLYYPRVAGGRTLAFYPHREGDAWETGP
                     YGILEPSNPTGVEPRLSGWDIIVVPGLAFDRRGNRLGRGFGYYDRFLGGLPESVPRVG
                     LSWAGQLVPEVPVDAWDVPVHALVTEEGVIRVVETSGSPET"
     gene            complement(2813..2993)
                     /gene="ssrS"
                     /locus_tag="VF357_09710"
     ncRNA           complement(2813..2993)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VF357_09710"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT          468 a         1125 c          913 g          563 t
ORIGIN      
        1 tccagtcgct ccgccgcatc cctgcggccc gcgacacttg gccatccctg gccatcgtcg
       61 tctgcgccgc cgctctgcgg aaccccgatg cccatggacg ggcaagtgcc gcgagagcca
      121 tggatggcgg gagcggcccc gctgcgccct gcgtcccggt accacggagg cacatcggag
      181 agggataccc cttcctcgct acgttccggc agcccccccg ccccagtacg agctccgcgc
      241 cgctggggta cccccgcagt aagaaaaacg gggggcacga gcccgaagtc ccattcccca
      301 ccggtcacat gttgccaaaa ttaagaaatg gagcacttgg cctcccctga cgcaaacctc
      361 agccatgcct ctctacagag ccgtccctag cgcgcgtact cgatggcccg ggtctccctg
      421 atcaccgtga cgcggatctg ccccgggtag gagaggtccg tctcgatctt cttcgcgatg
      481 tcgcgggcga gaagagtcgc cgcgtcgtcc ccgatcttct ggtagtcgac gatgatccgg
      541 atctcccgcc ccgcctggat cgcgtacgac ttctccacgc ctgcgaacga cttcgcgatc
      601 gcttcgaggt cctccagacg cttgaggtag ttcgccagca tctcgcggcg cgctcccggg
      661 cgggcgccgg acagcgcgtc cgccgcctgg accaggatcg gcaggatgtc cttcggcgac
      721 tcgtcctcgt ggtgcgcccc gatcgcgtgc acgatcttcg cggactcgcc gtatttccgg
      781 gccaggtcgg ccccgatcag cgcgtgtggc ccctcgacct cgtggtcgac cgcctttccg
      841 atgtcgtgga gcagtcccgc gcgcttggcg accttggcgt tcagccccag ctccgaggcg
      901 atcatcccgc acaggaacgc cacttcgagg gagtgggtgt agatgttctg cccgtacgag
      961 gtccggtatt tcagcctccc gatcaacttc acgaggtccg cgtggacacc gtggatcccg
     1021 aggtcgaaga gcgcctgctc tcccgcctcg cggatcgtct cctcgatctc cttggcgacc
     1081 ttctcgaccg tctcctcgat ccgggccggg tggatccgcc cgtcctggag aaggcgggtc
     1141 agcgagatcc tcgcgatctc ccggcggacc gggttgaaac cggacaggat gaccgcctcg
     1201 ggggtgtcgt cgatgatgac gtcgatcccg gtggcggctt cgaaggcgcg gatgttcctc
     1261 ccctcgcggc cgatgatccg ccccttcatc tcctcggacg gcaggggcac cgcgctcacg
     1321 acgtgctcgg cgacgtagtc cgccgcgtac cgctggacgg ccagggcgat catcttccgg
     1381 gccttctgcg tcgcctcctc cttgaactca tcgtccatga cgcggatcct cttgccggct
     1441 tccatcttgg cctcatcggc catcaactcc acgatctccg ccttcgcctg ctcggccgaa
     1501 aggcccgcca cccgctcgag ctcgacccgg gacgccgcca gccgggcgtc gagatccgcc
     1561 tgggccgcct cgagcttccg gtcctgttcc gcgatctccc gctccttttt cgtgtactcg
     1621 gcggcacgcg catcggcctg ctcgttcttg cggtcgagct ggtcctcttt ctggagaagg
     1681 cgcttctcga gctggctcag ctcgttcttc cggtcccgcg tctccttctc gaagtcgatc
     1741 tttacctgca acagatggtc cttggcctgg agggcggcct ccttcaggat gttcgtggcc
     1801 tccttcttgg agctctggag gatctccgcc gccttccccg cctcggcccg cgcgtcggcc
     1861 cgagccttgt ccaaagtgct ttttttcccg gaaaccagga acgccgcgta cacgccgagc
     1921 cccacggcca gcgccgcaag gaccgcaatg agcaccgtcg acatattcaa ggtgtgcctc
     1981 ctcggtaaaa catctatgtt tcaggggatc cggacgtttc cacgacacgg atcacccctt
     2041 cttccgtcac cagcgcgtgg acggggacgt cccacgcgtc gaccggcact tccggaacca
     2101 gctggcccgc ccaggatagc ccgacccgcg gcacgctctc cggcaggcct ccgaggaacc
     2161 ggtcgtaata tccgaacccg cgcccgagac gattcccccg ccgatcgaag gcgaggcccg
     2221 ggaccacgat gatgtcccac cccgacagcc gcggctcgac gcccgttggg ttcgacggct
     2281 cgaggatccc gtacggcccc gtctcccacg cgtccccttc ccggtgcggg tagaaggcga
     2341 gcgttcgccc cccggcgaca cggggatagt agagccgggc tcccgccgca agatacgcgt
     2401 gccggatccg ttcggtaggg acctcgccgg ccagcgcgca gtacagggcc gccgccatcc
     2461 ccgcccgcgg cggaaactcc cggagaaagc gggcctgcgc acggtcgccg gcctccgccg
     2521 aaaccgccga ctccgcccgt tcccgtggcc ggatacggct ttccctgcga agaatctcct
     2581 tacgctccaa gacgagcatc ggacgccttt caacggaggt ggacgggagg cgaaaaccgc
     2641 ggggttgctg cgctcgcgct gcggagatgt atcggcctat gacgccgggt acccttattc
     2701 gtacaaaaat tgccgccgca tcgaaccgcg ataagaacgt cgccgcaaca acggttatag
     2761 ccgccctgat ctatgcaaac gggcatttcc cgcccgggtc cgtctttgaa aaagtgcccc
     2821 cgcgaatgcc gtgtcgccgg tcgagttgaa cctggctttc accaggtggg ttcccggttg
     2881 ggccgcttca ggcttccccg gcgtaaggcg atgcacacca ccgcccggaa gggatcccgt
     2941 tctctaagtg ttggctcaaa ctctgccaac gatcacgcac acggcagggg ccccgacgaa
     3001 tttcctgcca gcctatttca acgcgtcgtc gagcctcgcc aggaggtccc ggtttttccg
     3061 gtccacccg
//
LOCUS       DASVJL010000467         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1491926, whole genome shotgun sequence.
ACCESSION   DASVJL010000467 DASVJL010000000
VERSION     DASVJL010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491926"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VF357_09715"
     CDS             <1..394
                     /locus_tag="VF357_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524468.1"
                     /translation="AAAITGGNPFGSMMQAWKYSLPAFLVPFFFSTTPVGYSLLIVGG
                     NWPDFLLATVTSLCSLFFVSLGIIGYLRKEVPVVERALLIMVAIAMVVFPIGWSIPGL
                     APAVIGGLMVVHQLRVTSGPVLDNEAQA"
     gene            433..1434
                     /locus_tag="VF357_09720"
     CDS             433..1434
                     /locus_tag="VF357_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7524469.1"
                     /translation="MNARGSRPFVVAALILAVSMGLAADAGAQPKQEKLSFSIVTGGT
                     GGVWYPLGGAIGGVIGKYVPNTEATSEATTAAIDNMKLLGAGKAGMAFAYDYHIGWAN
                     AGKVPGISAKHKIRLVMGFYEQPLHIVTKDGMGITSVLQLKGKRVSVGAPNSGTEEQA
                     DYVLKALGIDWNKDLKKEKLGAGESVAALKDGKIDAFFWSGAVPTASIIDMASTPGAK
                     MVLLPVGGEAAARIMKANPGVFHKTVFPKGCYAGVDRDIDAIAITAVLQAMDTFPADR
                     LYQILNAIFDNKAELTAVWKGAAALTPQKAVGLVTPDSLKYLHPGARKFFTEKGVLK"
BASE COUNT          298 a          495 c          519 g          291 t
ORIGIN      
        1 ggccgcggcg atcaccggcg gaaacccctt cggatcgatg atgcaggcct ggaagtattc
       61 cctccccgcc ttcctggtcc ctttcttctt ttcgacgacc cccgtcgggt acagcctcct
      121 gatcgtgggg ggaaactggc ccgattttct cctggccacc gtgacgagct tatgctccct
      181 gtttttcgtc tccctgggca tcatcggata ccttcgcaag gaggttcccg tcgtcgagcg
      241 ggcgctgctc atcatggtgg caatcgcaat ggtggtcttt cccatcggct ggtccatacc
      301 ggggctggcg ccggcggtca tcgggggcct gatggtcgtg catcaactac gggtgaccag
      361 tggccccgtc cttgataatg aggctcaggc atgaaggatc gggatgttcc ataccatccg
      421 gggaggtgga caatgaacgc gagaggatcc aggccattcg tcgtcgctgc gcttatcctc
      481 gccgtgtcga tgggacttgc ggcggacgcg ggcgcccaac ccaaacagga gaagctttcc
      541 ttcagcatcg tcaccggcgg caccggcggc gtctggtatc cgctgggcgg tgcgatcggc
      601 ggcgtcattg gcaaatacgt tcccaacacc gaggcgacct ccgaggccac gaccgccgcg
      661 atcgacaaca tgaagctgct gggcgccggg aaggcgggga tggcgttcgc atacgactac
      721 cacatcggct gggcgaacgc gggcaaggtg cccggcatct ccgccaaaca taagatccgc
      781 ctggtgatgg gattctacga gcagccgctc cacatcgtga ccaaggacgg catgggcatc
      841 acctccgtgt tgcagctcaa gggcaagcgc gtctccgtcg gcgccccgaa cagcggcacg
      901 gaggaacagg ccgactacgt gctcaaggcc ctcggcatcg actggaacaa ggatttaaag
      961 aaggagaaac tcggggccgg cgagtccgtc gcggcgctga aggacggcaa gatcgacgcc
     1021 ttcttctgga gcggcgccgt cccgaccgcg tccatcatcg acatggcctc cacccccgga
     1081 gccaagatgg ttctgctgcc agtgggcggc gaggccgcgg ccaggatcat gaaagcgaat
     1141 cccggcgtgt tccacaagac ggtcttcccg aaagggtgct acgcaggcgt ggatcgcgac
     1201 atcgatgcaa tcgcgatcac ggccgttctc caggcgatgg acaccttccc ggcggaccgg
     1261 ctctatcaga tcctgaatgc gatcttcgac aacaaggccg aattgactgc ggtctggaag
     1321 ggcgccgctg cgctgacgcc gcagaaggct gtggggctgg tgaccccgga ttcgctgaaa
     1381 tacctccacc cgggcgcgcg gaaattcttc acggaaaagg gcgtgctgaa ataaggccgc
     1441 atgcggaact tgggtagcgg gaattgcgcc gaaggggacc gatcatcgcc ggaatcgcgg
     1501 cttccttcct cgcccttggg ctgctgtcgc ggatcgttcc gtcggggtac acgcggatcg
     1561 acctgacgga ggacgggaag gggagggata tcttctccgc cgt
//
LOCUS       DASVJL010000468         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1866427, whole genome shotgun sequence.
ACCESSION   DASVJL010000468 DASVJL010000000
VERSION     DASVJL010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866427"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VF357_09725"
     CDS             complement(<1..1160)
                     /locus_tag="VF357_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEX7524470.1"
                     /translation="MTAWIQRIDTLLPDFSFAQEEAMVKMQEWARDDRERRLVRAVYR
                     HSGIERRHSVLRNYDGEGEGAFFRRDAGGALRGPGTAARNDIFSAESRAMSVALARKV
                     IGNCRGVTPADVTHVVTASCTGFYNPGPDYYIVRELGMSDATQRYHLGFMGCYAAFPA
                     LRMAAQFCAADPSAVVLVMCLELCSLHLQLNGSEDSLLANSLFADGAGAAIVSAREPE
                     PGTPAYRLEGFRSALVPAGEQDMTWRIGDQGFDIALSSYVPKLIGSNIRELVGPALAA
                     GGLSLPDVDTWAVHPGGKSIVDQVQRSLGLTADQVSASREVLRRCGNMSSATILFVLE
                     EILKRSSGKGRERVCAVAFGPGLTVEMATLEAIFAPDAAGVAAAAAVPLSSG"
     gene            complement(1194..1814)
                     /locus_tag="VF357_09730"
     CDS             complement(1194..1814)
                     /locus_tag="VF357_09730"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7524471.1"
                     /translation="MDMRAGILLAALVAVISSSSAGGAKADAPASGTIRGACEVAFLA
                     TSTLHDVSGSARCLPFETVLARGTAGRQILPTVEVEVPVASMDTRNKSRDGKMREMFL
                     SERFPRIHAAAHDVDVERLRVEIGKSRDGNASIDLLLRIRDVERKVRATASNLKESGE
                     RVSFDLEFPVSLREFDLKAPSVLGIIRVGDKVSVKATFALTVSRSP"
BASE COUNT          377 a          676 c          663 g          315 t
ORIGIN      
        1 catcccgacg acagagggac cgcagcggcg gcggcgaccc cggcagcatc cggagcgaag
       61 atcgcttcca aggtcgccat ctcgacggtc aaccccggcc cgaaagcgac ggcacagacc
      121 cgctccctcc ctttcccgga ggaacgcttg aggatctcct cgaggacgaa gaggatggtg
      181 gcgctgctca tgttcccgca ccggcgcaga acctcgcggg aagcgctcac ctggtcggcg
      241 gtaaggccca gcgacctctg cacctggtcg acgatcgact ttcccccggg gtggacggcc
      301 cacgtgtcga cgtcggggag ggacagtccc ccggcggcga gcgcgggacc gaccaactcg
      361 cggatgttcg agccgatgag cttcgggacg tagctcgaca gggcgatgtc gaacccctgg
      421 tcgccgatcc gccacgtcat gtcctgctcg ccggcgggta ccagcgcgga acgaaacccc
      481 tccagtcggt acgcgggcgt accgggctcc ggttcccggg cggaaacgat cgcggccccc
      541 gccccgtcgg cgaagagcga gttcgcgagc aggctatcct ccgatccgtt caactggagg
      601 tggaggctgc acagctcgag gcacatcacg aggaccacgg cggacgggtc cgccgcgcag
      661 aactgggcgg ccatccgcaa ggcggggaac gccgcataac accccatgaa gccgaggtgg
      721 taccgctgcg tggcgtccga catccccagc tcgcgcacga tgtagtagtc gggcccgggg
      781 ttgtaaaacc cggtgcacga cgcggtcacg acgtgcgtca catcggcggg ggtgaccccg
      841 cgacagttcc cgatcacctt ccgggccaac gccaccgaca tcgcgcggga ctccgctgaa
      901 aagatgtcgt tgcgggcggc ggtcccggga cctcgaaggg ctccaccggc gtcgcgccgg
      961 aagaaggcgc cctccccctc cccgtcgtag ttgcgaagga cggagtggcg gcgctcgatc
     1021 cccgagtgcc ggtacacggc gcgcaccagg cgccgctccc gatcgtcgcg agcccactcc
     1081 tgcatcttca ccatcgcctc ctcctgggcg aaggagaagt cggggagcag cgtgtcgatc
     1141 cgttggatcc aggcggtcat gggttgcctc cgtctattac gattggatgc gtctcaaggg
     1201 gatcgggaaa cggtcagggc gaaggtggcc ttgacggaga ccttgtcgcc gacccggatg
     1261 atgccgagga ccgagggggc tttcaggtcg aactccctga gggacacggg gaattcgagg
     1321 tcgaagctga cccgttcgcc ggactccttc aggttgctcg ccgtggcgcg gaccttccgc
     1381 tcaacgtccc ggatccggag gagcaggtcg atggaggcat ttccatcgcg gctctttcca
     1441 atttccactc gaagccgctc aacgtctaca tcatgcgccg ccgcgtggat tcgcggaaac
     1501 cgctccgaca ggaacatctc ccgcatcttc ccgtcgcggg atttgttacg ggtgtccatt
     1561 gaggccaccg gcacttcgac ttccacggtc gggaggatct gcctgcccgc ggtgccgcga
     1621 gcaaggaccg tttcaaaggg caggcagcgg gcggaaccgg aaacgtcgtg cagggtggac
     1681 gtggcgagga aagcgacctc gcacgctccc cggatcgtcc ccgaagcggg ggcgtccgcc
     1741 ttcgctcccc cggcggagga ggaagaaata acggcaacca gcgccgccag cagtatcccc
     1801 gcgcgcatat ccatccaggg tcacctcttc agacaatgcc atcgtggaaa aggtcggggg
     1861 cgttcgatcc gattaagaac aggttacact aaaggaacac agatgctctg gataccggaa
     1921 agaaaaagcc tgcccgagtt gctggacctt ccgcacgact ccttcgcgcg tgatgaactc
     1981 gagggggccc tcaatgacct gaccctcgtg aatcactacc tcggtaatgg c
//
LOCUS       DASVJL010000469         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_270493, whole genome shotgun sequence.
ACCESSION   DASVJL010000469 DASVJL010000000
VERSION     DASVJL010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270493"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VF357_09735"
     CDS             complement(<1..279)
                     /locus_tag="VF357_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524472.1"
                     /translation="MKTSMENESPYRRLKRFLFGAPRDLFDPKIFHNISLIAFFAWVG
                     LGADGITSSCYGPEEAFLALGQHSVLAIFVAVMTVGTILIVSGSYSQII"
     gene            complement(331..900)
                     /locus_tag="VF357_09740"
     CDS             complement(331..900)
                     /locus_tag="VF357_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7524473.1"
                     /translation="MVDLERYPRKITLHNGVTLVFRPMDRYDVGRLWVFFQQIPPEDK
                     MFFRQDVSRREVVQHWADTLDYDLVLPILALEGDRVVGDATLHRQKTGWKQRVGVVRV
                     QIAPDYRHIGLGTTMVRELRHLGEKSALHYLMAEVIEEQAGAVRAFEKMGFVRGAMYR
                     NFVNDQRGGLHNLLVLLYRMSSSSDGLDV"
     gene            complement(928..1410)
                     /locus_tag="VF357_09745"
     CDS             complement(928..1410)
                     /locus_tag="VF357_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524474.1"
                     /translation="MKGTDYHCPHCGAELRGDRRKGGSRPGGAPGCPYDALAYAALRA
                     GHDAIYFGAWRRIDALPLEIRRAYHGIGRHLDAIGRELAVHDLPAAIRDLNEAIKAHR
                     TADPREEAPDVLRLMDNALSYAHRAIDDLLHEKGLPPHQPMDFAEWYDVVEVPFRDEW
                     "
     gene            complement(1452..>2044)
                     /locus_tag="VF357_09750"
     CDS             complement(1452..>2044)
                     /locus_tag="VF357_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524475.1"
                     /translation="GHSETLWGISEAFRLEQREKIFYYRKPRAESVPPRPAAAPRSSG
                     SSAPLRAIPRAGPPPSPVDDSSSAMKLVLSAERMADEGRFEDAVAMCRQAAALDPECP
                     EPEYLMAFLLRGNGRHAEALYHAERALERDPRFVFADMEAAECLCGMGQAGEATLRWK
                     GILRAIEGNVHLPRLSVGAGISPQMLRQYVLSRLPR"
BASE COUNT          358 a          691 c          668 g          327 t
ORIGIN      
        1 gatgatctgg gagtaactgc cggagacgat caggatcgtc cccaccgtca tcaccgcgac
       61 gaagatggca agcaccgagt gctgcccgag ggcgaggaac gcctcttccg gaccgtagca
      121 cgaggaggtg atcccgtccg cgcccaaccc cacccaggca aagaaggcga tgagggagat
      181 gttgtggaag atcttcgggt cgaacagatc ccggggggcg ccgaacagga accgcttcaa
      241 tcgccggtac ggagactcgt tttccatcga tgtcttcatc ttcccgggag cggatttcgg
      301 ggccggccga aggtaggagt atacaacggt tcagacgtcg agaccatctg aggaggagga
      361 catccggtac agcagaacca gcaggttgtg cagccccccc ctctggtcgt tgacgaagtt
      421 ccggtacatc gccccgcgga cgaagcccat cttctcaaag gcgcgcaccg cgcccgcctg
      481 ctcctcgatc acctcggcca tgaggtaatg gagggccgac ttctccccga ggtgacgcag
      541 ttcgcggacc atcgtggtcc cgagcccgat gtgccggtaa tcgggggcga tctggacgcg
      601 caccaccccg acccgctgct tccatcctgt cttctggcgg tggagcgtgg cgtcccccac
      661 aacgcggtcc ccttcgagcg cgaggatcgg gaggacgagg tcgtagtcga gggtgtccgc
      721 ccaatgctgc acaacctcgc ggcggctcac gtcctggcgg aagaacatct tgtcctcggg
      781 ggggatctgc tggaagaaga cccacaaccg gcctacgtcg taccgatcca tcggccggaa
      841 aaccagcgtc accccgttgt gcagggtgat cttcctcggg taccgctcga gatcgaccat
      901 ctcagggtcc ctccgcggat tccgtgtcta ccactcgtcc cggaacggga cctcgacgac
      961 atcgtaccac tccgcgaaat ccatcggctg gtgcggcggg agcccttttt cgtggaggag
     1021 gtcgtcgatc gcccggtggg cgtaggagag ggcgttgtcc atgaggcgaa gaacgtccgg
     1081 ggcctcctcc cggggatcgg cggtccggtg cgccttgatc gcttcattga ggtcccgtat
     1141 ggcggcgggg aggtcgtgga ccgccagttc tctcccgatc gcgtcgaggt ggcggccgat
     1201 cccgtgatac gcccgccgga tctccagggg aagggcgtcg atgcggcgcc acgccccgaa
     1261 gtagatcgcg tcgtgcccgg cccgcaaggc cgcgtacgcc agcgcgtcgt aagggcatcc
     1321 gggcgccccc ccggggcggg agccaccctt ccgcctgtcg ccccgcagct ccgcgccgca
     1381 gtggggacaa tggtaatcgg tccccttcat ccggagatta tctcagcggg aaggggcccc
     1441 cgcaacggta ttcatcgcgg caatcgggag agcacgtatt ggcgcagcat ctgcggggag
     1501 attcccgccc ccaccgaaag acgcggaagg tgcacgttcc cctcgatggc gcgcaggatc
     1561 cccttccacc gcagcgtcgc ctccccggcc tgccccatcc cgcagaggca ctccgccgcc
     1621 tccatgtccg caaagacgaa ccgcgggtcc cgttcgagag cgcgctcggc gtgatagagc
     1681 gcctccgcgt ggcgtccatt tccgcgaaga aggaacgcca tcaggtattc gggctcgggg
     1741 cactccggat cgagggccgc cgcctggcgg cacatcgcga ccgcatcctc gaagcgcccc
     1801 tcgtcggcca tccgctccgc cgaaaggacc agtttcatcg cggaagagga atcgtccacg
     1861 ggagaaggag gaggccccgc gcgcgggatc gcccgcagcg gggcgctcga tccggaggaa
     1921 cggggcgcgg cggcaggacg cggcgggacg ctctccgcgc gcggtttccg gtagtagaaa
     1981 atcttctccc tctgctccag ccggaacgcc tccgaaatcc cccacaacgt ttcggagtgt
     2041 ccga
//
LOCUS       DASVJL010000470         1579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1210553, whole genome shotgun sequence.
ACCESSION   DASVJL010000470 DASVJL010000000
VERSION     DASVJL010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210553"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VF357_09755"
     CDS             complement(<1..197)
                     /locus_tag="VF357_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524476.1"
                     /translation="MARRIAHRILIALALLSAVPGCGYRLESGTARFKDPSVRMDVSS
                     FANRSTTPDAGAVVATRFREEL"
     gene            complement(224..>1579)
                     /locus_tag="VF357_09760"
     CDS             complement(224..>1579)
                     /locus_tag="VF357_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7524477.1"
                     /translation="LEGNGMGRGTIQYRLRDWGVSRQRYWGCPIPVIHCEACGIVPVP
                     EKDLPVVLPEDLPYTRERGNPLDGAEKWLRVPCPSCGKPGRRETDTFDTFVESSWYFL
                     RYIDPKNDRAPLDPEKMRRFAPVDQYVGGVEHACMHLIYARFFHKYLRDRGLAPGNEP
                     FERLLSQGMVCMQTAECPKHGWRYPEEVDEKGKCRQCGETVDVGRSMKMSKSKRNVVE
                     PSTLIERYGADTARLFILFAAPPEKDLDWNEQGVEGAFRFLNRIYRLVAPRAKAIADA
                     PAAWDGSEEVRAIRQVTHRTLIKVTGDIEDRSHFNTAISAVMEMVNFLYLVPEAAWGR
                     PATAAALREAVEILLHMLSPFAPHMGEELWERIGGKGLLCSSSWPAADVDVAREEEVE
                     VVVQVNGKVRSKLTVGAGAGEGEVRERVMADPKIREYMAGKEVRKTVYVSGKLFSIVV
                     S"
BASE COUNT          261 a          542 c          502 g          274 t
ORIGIN      
        1 agctcctctc gaaagcgcgt ggcgacaacg gcaccggcgt cgggcgtcgt ggaacggttc
       61 gcgaaggagg agacatccat tcgcaccgag ggatccttga atcgcgcggt cccggactcg
      121 aggcggtatc cgcaccccgg aaccgcggac aggagcgcca acgcgatgag aatccgatgc
      181 gcgatccgac gtgccatgaa attcccctgg tgcttcaatt cattcacgaa actacgatgc
      241 tgaagagctt gccggaaacg tagacggtct tccggacctc cttgcccgcc atgtactccc
      301 ggatcttcgg gtcggccatc acccgctcgc ggacctcgcc ctctccggcc ccggcgccga
      361 cggtcagctt cgaccggacc ttcccgttga cctggacgac gacctcgacc tcctcttcgc
      421 gcgcgacgtc gacgtcggcg gcgggccagg aactggagca gaggagtccc ttccccccga
      481 tccgctccca tagttcctcg cccatatgcg gcgcgaaggg agagagcatg tgcagcagga
      541 tctccaccgc ttcgcggagg gcggcggcgg tcgcggggcg gccccatgcg gcctcgggaa
      601 cgaggtagag gaaattcacc atctccatga cggcggagat cgccgtgttg aagtgggaac
      661 ggtcctcgat gtcgccggtg accttgatca gtgtccggtg ggtcacctgg cggatggcgc
      721 gaacctcctc cgagccgtcc cacgcggccg gggcgtccgc gatcgccttc gcgcgcgggg
      781 cgaccaggcg atagatgcgg ttgaggaacc ggaacgctcc ctccaccccc tgttcgttcc
      841 agtcgaggtc cttttccggc ggcgccgcga agaggatgaa gagtcgggcc gtgtcggccc
      901 cgtaccgctc gatcagcgtg gagggttcga cgacgttccg cttcgacttg gacatcttca
      961 tcgaccgccc gacgtcgacc gtctctccgc actggcggca tttccctttt tcgtcgacct
     1021 cctccgggta cctccagccg tgcttggggc actcggcggt ctgcatgcag accatcccct
     1081 gggaaagcag gcgctcgaac ggctcgttcc cgggggccag ccccctgtcg cggagatact
     1141 tgtgaaagaa gcgggcgtag ataaggtgca tgcaggcgtg ctccaccccc cccacatact
     1201 ggtccacggg cgcgaatcgg cgcatctttt ccgggtcgag cggcgcgcgg tcgtttttcg
     1261 ggtcgatgta cctcaggaag taccaggagg actcgacgaa ggtgtcgaac gtatcggtct
     1321 cccgccgacc gggcttcccg caggaggggc acggcacgcg gagccatttc tccgcgccgt
     1381 ccagcgggtt cccccgctcc cgcgtgtacg gaaggtcctc cggaagcacc accggaaggt
     1441 ccttctccgg gacggggacg attccgcacg cctcgcagtg gatcaccggg atcggacaac
     1501 cccagtaccg ctgccggctg acgccccagt cgcgaagacg gtactggatc gtcccgcggc
     1561 ccatcccatt tccttcaag
//
LOCUS       DASVJL010000471         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1021427, whole genome shotgun sequence.
ACCESSION   DASVJL010000471 DASVJL010000000
VERSION     DASVJL010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021427"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VF357_09765"
     CDS             <1..186
                     /locus_tag="VF357_09765"
                     /inference="COORDINATES: protein motif:HMM:NF018652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524478.1"
                     /translation="LRMSEGVSPPSIEKRNGPPPDEVREAVENLIGSGLLLREGDRLR
                     LPERRWFVSNEVLQRLA"
     gene            complement(191..694)
                     /locus_tag="VF357_09770"
     CDS             complement(191..694)
                     /locus_tag="VF357_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524479.1"
                     /translation="MTNSRSVFLCMLAGAAIAVASGFSEPARAEVSVNINMGPPVVVA
                     EPPHMVLVPRSRVYFSPDPQVDIFFYGGYWWSPRGDRWYRARAYNGPWGVIERRRVPR
                     DVIVVPRDYRRRFEREKPIPYGQWKREHFKEHGKAERKERKRDEREGKERENHDRDDG
                     GRGNHGR"
     gene            864..2210
                     /locus_tag="VF357_09775"
     CDS             864..2210
                     /locus_tag="VF357_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7524480.1"
                     /translation="MGIRLKLFGFLLPIVFLLIVTVTWVVTEVTKSTLRRDLLQRGAA
                     ISRVVALSAGHSLLANDPLGLDRLVSETRESDRDIAFVAIRDASDIVVAHDRVTERGK
                     PYNPVPPLLPLGTFGDTRADEVVRDGRRLIEYTTPISFAGRRIGIASVGLSMQGLADA
                     QRSIRQMIFAAAAVILVAAFFGTLLLSSFITTPVKRLHQGVLSLASSETFQPVPARSS
                     DELGALTRNFNRMAETILAQKTALQKKALQLEEAYVSMVRVIAASLDARDPYTMGHST
                     RVARISCALGRRLGMGEEELSNLERAAIFHDIGKIQTPDDVLLKGERLSRAEEEQMKG
                     HPVDGSEILRMAPFLQRYIPVVRAHHEWYNGEGYPDGIKGDEIPLHAQLIALADAFDA
                     MTTNRPYRQALSEEAAITEILRFRGTQFSPELADAFAKMMREMPRMDETILKSMAL"
     gene            2207..>2802
                     /locus_tag="VF357_09780"
     CDS             2207..>2802
                     /locus_tag="VF357_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524481.1"
                     /translation="MRRKTLPPILLLSVLAATAFSGCGWHSKTSITTPSAEPHTAAPI
                     EPAAKPHTAAPIEPAPRPPAPAISASDKLFAEGMTALQEGGHERALELFSAAWKEKPG
                     HTGVAREFDGALLALKKNGDAAYGQGKWEDAGKRWMGTLRFINHPAANWRGYLFTRSE
                     VQAKVDRLSAALTKKALTEYRREEVPLAIADWKTILSY"
BASE COUNT          495 a          944 c          888 g          475 t
ORIGIN      
        1 ctgcggatgt cggaaggggt gtccccgcct tccatcgaaa aaaggaacgg ccccccgccc
       61 gacgaggtgc gggaggccgt ggaaaacctg atcggatccg gcctgctgct ccgggaggga
      121 gatcgcctgc ggctaccgga acggagatgg ttcgtctcga acgaggtgct tcagcgactg
      181 gcctgaaccg tcatcgtccg tgatttccgc gtcctccgtc gtcgcggtcg tggttctccc
      241 gttccttccc ttccctctcg tcgcgcttcc gttccttgcg ctccgccttc ccgtgctcct
      301 tgaaatgctc cctcttccat tgcccgtacg ggatcggctt ctcccgttcg aagcgcctcc
      361 gataatccct ggggacgacg atcacgtccc gcggcacacg gcgccgttcg atcacccccc
      421 acggaccgtt gtacgccctc gcccggtacc accgatcgcc ccgcggcgac caccagtacc
      481 cgccgtagaa aaagatgtcc acctggggat ccggggagaa atagacccgg gaccggggga
      541 cgaggaccat gtgcggcggc tccgcgacca cgaccggcgg acccatgttg atgttgacac
      601 tcacttccgc ccgcgcgggc tccgaaaaac ccgatgcgac ggcaatcgcc gcccccgcca
      661 gcatgcacag gaaaaccgat ctcgaattcg tcatgtgccg cctcccttct tgtcgtcgtg
      721 ggtaagaccg cccctcggtc cattttattc attccgccga tattgaaccc acggaccgtc
      781 tcgtgactct atatagtaat atgactgcca tggatgccga ggaaaaaggg acagggccgg
      841 ggggaatcga acggagagtc ggggtgggta ttcgtctgaa actgttcggg ttcctgctcc
      901 ccatcgtctt cctcctgatc gttaccgtga catgggtggt cacggaggtt accaagtcca
      961 ccctgcgccg tgacctgctc cagcggggcg ccgcgatctc ccgcgtggtg gccctctccg
     1021 cgggacattc cctgctggcg aacgatccgc tggggctgga ccggctggtc tcggaaacca
     1081 gggagagtga tcgcgacatc gccttcgtcg ccatccggga cgcctccgac atcgtggttg
     1141 cccacgaccg ggtgacggag aggggaaaac cgtacaatcc cgttccgccc ctgcttccgc
     1201 tgggaacctt cggcgacacg cgggcggacg aggtggtgcg cgacggacgg cgtctcatcg
     1261 aatacaccac cccgatctcc ttcgcgggac ggcgcatagg gatcgcctcc gtcgggctct
     1321 ccatgcaggg tctcgcggac gcgcagcggt cgatccggca gatgatcttc gccgcagcgg
     1381 cggtcatcct ggtggccgcc ttcttcggaa cgcttctgct ctcgtcgttc atcaccaccc
     1441 cggtgaaacg gctccaccag ggagttctct ccctcgcgag cagcgagacg ttccagcccg
     1501 tcccggcgcg ctcctccgac gaactgggcg ctctcacgcg gaacttcaac cggatggcgg
     1561 agacgatcct cgcccagaag accgccctcc agaagaaggc gcttcagttg gaggaagcgt
     1621 acgtcagcat ggtccgcgtc atcgccgcgt cgctggacgc gagggacccg tacacgatgg
     1681 ggcactccac ccgcgtggcg cggatatcgt gcgcgcttgg acgccgcctc gggatgggcg
     1741 aggaggagct ttccaacctg gagcgggcgg cgatcttcca cgacatcggg aagatccaga
     1801 cccccgatga cgtcctcctc aagggggagc gtctgtcgcg cgccgaggaa gagcagatga
     1861 aaggccatcc cgtcgacggc tcggagatcc tccggatggc gccgttcctc caacgctaca
     1921 tacccgttgt gcgcgcccac catgagtggt acaacgggga ggggtatccg gacggaatca
     1981 agggggacga gattccgctc cacgcccagc tcatcgcgct ggccgacgcc ttcgacgcga
     2041 tgaccaccaa ccggccttac cggcaggcgc tttcggaaga ggcggcgatc accgagatcc
     2101 tgcggttccg cgggacgcag ttctccccgg aactggccga cgccttcgcg aagatgatgc
     2161 gggagatgcc gcggatggac gaaaccatat tgaagagcat ggcgctttga gacgaaagac
     2221 ccttccgccg atcctcctcc tgtcggtcct cgccgcaacc gctttttcgg ggtgcgggtg
     2281 gcattcaaaa acctccatca cgactccgtc cgcggaaccg cacaccgccg cacccatcga
     2341 gcccgccgcg aagccgcaca ccgccgcacc catcgagccc gccccgaggc cgcccgcgcc
     2401 cgcgatctcc gcgtccgaca agctgttcgc cgaggggatg acggcgctgc aggagggcgg
     2461 gcacgaacgg gccctcgaac tgttttcggc ggcctggaag gagaagccgg gccatacggg
     2521 agtggcccgg gagttcgacg gcgcgctgct cgccctcaag aagaacgggg acgcggcgta
     2581 cgggcaggga aagtgggaag acgcggggaa acgatggatg gggacgctgc ggttcatcaa
     2641 tcatcccgcg gcgaactggc gtggctacct tttcacgcgg agcgaggtgc aggcgaaggt
     2701 cgaccgtctc agcgcggcgc tgacgaagaa ggcgctgacg gagtaccgcc gggaggaggt
     2761 tccgttggcg atcgccgact ggaagacgat cctttcctac ga
//
LOCUS       DASVJL010000472         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1493215, whole genome shotgun sequence.
ACCESSION   DASVJL010000472 DASVJL010000000
VERSION     DASVJL010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493215"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VF357_09785"
     CDS             complement(<1..608)
                     /locus_tag="VF357_09785"
                     /inference="COORDINATES: protein motif:HMM:NF028174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HEX7524482.1"
                     /translation="MGPVRNVDELMAVGHGYQRAMILFAALKLGVFRGLASGGCDASA
                     LARRVGADPGKLSILLDALAAQGLVGKKGKKYRNAQVARKHLLPGPRSMESILLHHFD
                     GWGEWGRLSGTVRAGRTPRGDAEGNFQENFIRGMEENARERAADVARRILIRRGDRVL
                     DLGGGPGTYAVAWADACPGAEITVFDTPATLRVTRKILREKGA"
     gene            complement(614..>1956)
                     /locus_tag="VF357_09790"
     CDS             complement(614..>1956)
                     /locus_tag="VF357_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524483.1"
                     /translation="LRTYLLTDLLVRTLRFLGYGTFTVQNITDMGHMHQEQLEAGEDK
                     VIVAARAAGKSAGEIARFFTEAFFRDGRRMAFLPADVSPRASEHVPEMISLVRALEER
                     RAVYGGDGYIYFDVTKAPGYGIFSGARLGEGEAAERTEVAIHRHKKRPEDFTLWIPAE
                     PDREFRWESRWGVGWPGWHIECAAMAIRYLGPEFDIHVGGIDLRFPHHENSRAVAVTA
                     TGKKFAALWLHAAHLLVDGKKMSKSAGNEYTLDDLAACPRSLGKGYSPADFRYYCLTL
                     HYSSPMNFTWEGQAAAARACTRLRAAFRRAALQATSGEDAAAVLRAEFRGAISDDLNM
                     PRALAVVHNAVRPGIGGDAARALATEWDTVLGVGLLSEADGDREALGVCEGGPEGVPP
                     VVEAMAREREIRRRARDYAEADTLREKIRAAGYDVVDAAEGTVVLRKMQETLRR"
BASE COUNT          297 a          741 c          600 g          318 t
ORIGIN      
        1 gctcccttct cccgaaggat cttccgcgtg acccgcaacg tcgccggggt gtcgaagacg
       61 gtgatctccg cgcccgggca ggcgtcggcc cacgcgacgg cgtacgtccc cgggccgccg
      121 ccgaggtcca ggacgcgatc cccccgccgg atcaggatcc gccgggccac gtccgccgcc
      181 cgctcacggg cgttttcctc catcccgcga atgaagttct cctggaagtt cccctccgca
      241 tccccgcgag gcgtccgccc cgcccgcacc gttccggaaa ggcggcccca ctccccccaa
      301 ccgtcgaagt ggtgcaggag gatggattcc atcgaacgcg gccccgggag cagatgtttc
      361 cgcgcgacct gcgcgttccg gtattttttc ccctttttcc cgaccagccc ctgggccgcg
      421 agggcgtcga gaaggatgga gagcttcccg gggtcggctc cgacccggcg cgccagcgcg
      481 gacgcgtcac atcccccgga cgcaagcccc cggaacaccc cgagcttcag cgcggcgaac
      541 aggatcatcg cgcgctggta gccgtgcccc accgccatca gttcatccac gttccgaacc
      601 ggtcccatcc gcgtcacctc ctcagggtct cctgcatttt ccgaagaacc acggttccct
      661 ccgccgcgtc caccacatcg taccccgcgg cgcggatctt ttcccgcagc gtatccgcct
      721 cggcgtaatc gcgcgcccgc cgtcggatct cccgttcccg cgccatcgcc tcgacgacgg
      781 gaggaacccc ctccggccct ccttcgcaga cgccgagcgc ttcgcgatct ccatctgctt
      841 cggagagcag ccctacgccg aggaccgtgt cccactccgt cgcgagcgcc cgggccgcgt
      901 cccccccgat tccaggccgg accgcattat ggaccacggc gagggcacgc ggcatgttca
      961 ggtcatccga gatcgcgccg cggaactccg ctctcaacac cgcggcggcg tcctccccag
     1021 aagtcgcctg cagcgccgcc cggcggaacg cggcccgcag gcgggtgcac gcgcgggcgg
     1081 ccgccgcctg cccctcccag gtgaagttca tcggcgagga gtagtgaagt gtgaggcagt
     1141 agtaacggaa atcggccggg gagtaccctt taccgaggga ccgggggcag gcggcgagat
     1201 catccagcgt gtattcgttc ccggcggact tggacatctt cttcccgtcg accagcaggt
     1261 gcgcggcgtg cagccagaga gccgcgaact ttttccccgt cgcggtcacc gccaccgccc
     1321 gggagttctc gtggtgtgga aagcggaggt cgattcctcc gacgtggata tcgaattccg
     1381 gaccgagata cctgatcgcc atcgccgcgc actcgatgtg ccatcccggc caccccacgc
     1441 cccagcggct ctcccaccgg aattccctgt cgggctcggc gggaatccag agggtgaaat
     1501 cttccgggcg cttcttgtgc cgatggatcg ccacctccgt gcgctccgcg gcctctcctt
     1561 ccccaagacg ggccccggag aagattccgt atccgggcgc tttcgtcacg tcgaagtaga
     1621 tgtatccgtc cccgccgtag acggccctcc gctcctcgag cgcgcgcacg agcgagatca
     1681 tctccgggac gtgctccgag gccctcgggg aaacgtccgc cggcaggaac gccatgcgtc
     1741 tgccgtcgcg gaagaacgcc tccgtgaaga accgggcgat ctcccccgcc gacttccccg
     1801 ccgctcgggc ggcgacgatc accttgtcct cgcccgcctc cagttgctcc tggtgcatgt
     1861 gccccatgtc cgtgatgttc tggacggtga acgtgccgta ccccaggaag cgaagggtgc
     1921 ggacgaggag gtcggtcagc agataggtgc gaaggt
//
LOCUS       DASVJL010000473         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1677582, whole genome shotgun sequence.
ACCESSION   DASVJL010000473 DASVJL010000000
VERSION     DASVJL010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677582"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VF357_09795"
     CDS             <1..1040
                     /locus_tag="VF357_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7524484.1"
                     /translation="IVEAQPLPRDGGILVVGKKEGETDLVLWERNARTVWHIEVGSGK
                     KSIAEDARGFAGAFPGVTVVEAGGSVILSGPVTTPQDKAVLEAYARAHPGVHLRLSLP
                     EEKKTLLLYDLKIIEISRGETAQLGIRWPDALPAKGTFAVGTGNAGTFSVGTDFEARL
                     NLLMANGKARILSNPRLACESGGEAQFLAGGEIPIVIITPETRTVEWKTYGIILKIHP
                     TMAEGGKIRTQVNAEVSAVDHGSGTSDVPGFLTRRVSTLFSTPPGETVMLSGLVKSEM
                     AKDVAKVPLLGQIPVIGELFKSRSFRENRTELAIFITPIVVAGDAGLEAANWEREAEK
                     EKEHLRFRLLD"
     gene            1101..>1976
                     /locus_tag="VF357_09800"
     CDS             1101..>1976
                     /locus_tag="VF357_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7524485.1"
                     /translation="MNVELRRTLHQAVLSRLDARKTGISYLEDVGRWRDRAEEYLQAE
                     MCGLSLEDAEQDALRQGILDEIFAFGPITPLLSDPAVSEIMVNGYESIYVERSGLLSR
                     NGGSFLSEESLRATIDRMVSKVNRRLDESSPYVDARLPDGSRINAIIPPVCLTGACLT
                     VRKFRKEAFSLEELVRIGSVDKDAADYLREAVRNRRNVIVSGGTGSGKTTLLNALSQF
                     IPEEERIVTIEDAAEIRLQKPHVIRLEARPVNIEGSGAVTIRDLVRNSLRMRPDRIIV
                     GECRGGEALDMLQAMN"
BASE COUNT          416 a          592 c          658 g          310 t
ORIGIN      
        1 agatcgtcga ggcccaaccc cttccccgtg acggcggaat cctcgtggtc gggaaaaagg
       61 agggggaaac cgaccttgtc ctgtgggaac ggaatgcgcg gacggtgtgg cacatcgaag
      121 tgggatcggg aaagaaatcg atcgccgaag atgcccgggg gttcgcggga gcctttcccg
      181 gcgtgaccgt cgtcgaggca ggcggatcgg tgatcctcag cggtcccgtg acgacgccgc
      241 aggataaagc cgtgctcgag gcgtatgccc gggcccaccc cggggtccac ctacggctct
      301 ccctgcccga ggaaaagaaa acccttctac tctacgacct gaagatcatc gagatcagtc
      361 ggggggaaac ggcacaactg ggcatccgct ggccggatgc gctgccggca aaaggaacat
      421 tcgccgtggg aacgggaaac gcgggaacgt tttccgtagg cacggacttc gaggcgcgac
      481 tgaacctcct catggcgaac ggaaaggcga ggatcctctc caatcctcgc ctcgcgtgcg
      541 aaagcggcgg ggaagcgcag tttctcgcgg ggggggaaat cccgatcgtg atcatcacac
      601 cggagacccg taccgtggag tggaaaacgt acgggatcat cctcaagatc caccccacca
      661 tggcggaagg gggaaagatc cgcacgcagg tgaacgcgga ggtaagcgcg gtggaccacg
      721 gaagcggaac gtccgacgtc ccgggatttc tcacccgacg cgtgtccacc ctcttctcga
      781 caccgccggg ggaaacggtg atgctgtcgg gtctcgtcaa gagcgagatg gcaaaagacg
      841 tcgcgaaggt cccgttgctg gggcagatcc ccgtgatcgg ggaactgttc aagtcgcgaa
      901 gcttccgcga gaaccggacg gagctcgcga tcttcatcac cccgatcgtg gtggccgggg
      961 acgccggcct tgaggccgcc aactgggagc gggaagcgga gaaagagaag gaacacctcc
     1021 gcttcaggct gctcgactga accgggaaat accgaggggg cacgagcacc gaagcactac
     1081 acaaatcagg aggaatccgt atgaacgttg aacttcgacg cacgctgcac caggctgtac
     1141 tgtcgcggct cgacgcgagg aagacgggga tatcgtattt ggaggatgtc gggcgctggc
     1201 gagaccgcgc cgaagagtac ctgcaggcgg agatgtgcgg attgtcgctg gaagacgcgg
     1261 aacaggacgc cttgcggcaa gggatcctcg acgagatctt cgccttcggg ccgatcactc
     1321 ccctgctctc cgaccccgcc gtctccgaga tcatggtgaa cggatacgag tcgatctacg
     1381 tggagcggag cgggttgttg tcccgcaacg gaggctcgtt cctctcggag gaatcgctac
     1441 gcgccacgat cgaccggatg gtgtcgaagg tcaaccgacg cctcgacgaa tcgtccccct
     1501 acgtcgacgc ccgactcccg gacgggtcgc gcatcaacgc gatcattcca ccggtttgcc
     1561 tcaccggcgc gtgcctgacg gtccgcaaat tccgaaagga ggcattctcc ctcgaggaac
     1621 tcgtccgcat cggttcggtg gacaaggatg ccgcggatta tctgagggag gccgttcgga
     1681 accggcgtaa cgtcatcgtc tcggggggga ccggctcagg gaagaccacc ctgctgaacg
     1741 ccctgtccca gttcatcccg gaggaagagc ggatcgtcac gatcgaggat gcggcggaga
     1801 tccggcttca gaagccccac gtgatccgac tcgaggcaag gccggtcaac atcgaaggat
     1861 cgggcgccgt caccatccgg gacctcgtcc gcaactccct tcgcatgcgc cccgaccgga
     1921 tcatcgtagg ggagtgccgc gggggggagg cactcgacat gctgcaggcg atgaat
//
LOCUS       DASVJL010000474         5642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1677695, whole genome shotgun sequence.
ACCESSION   DASVJL010000474 DASVJL010000000
VERSION     DASVJL010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677695"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2029)
                     /locus_tag="VF357_09805"
     CDS             complement(<1..2029)
                     /locus_tag="VF357_09805"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX7524486.1"
                     /translation="MPHTLPRLSFEHVALALAKRGTPPCVQWMRIPPGARSFLAARLF
                     ERLDRTLLYLCAGEKEAEEATRELTAYLGSDTVLPFPSIEATPYEPVPPHLPSVHDRM
                     RALHRLLSGPPAVVVAPIAAAVEKTLPPEVFVDAVGAVSTGDTLDVDAFAARLVTLGY
                     ARLPAAADPGDFAVRGGIVDVYSPAHPLPARLLLDGDVVESVRWFHPATQRTVAAGAR
                     ADGEPGDGERLVILPCSQVITRDDCLSAACDGPAEDVGGPVGMEQAPKGGRASGSFPW
                     KETLRQGIRFHGADALLPRLYGHAAPVFSYLPANALVVAVDSVECVAAARNTFEEAEE
                     MYALVREEEGYPAPSELVVPEAELVAALSGVPMLAFDGIEVPPFGRKEPLRGDAGMDG
                     NEEIRRSTATASSEGLLYPLAEEAKVWWKRGDRLIVSSLSPSQVDRMEDFLSRYAVPL
                     SRVDTLREALSRDRGVFLCGSEVTRGFRAPELRVAIVTESEIFGEKARARRPRKERIA
                     VPEEFSLADLRVNDPAVHVDHGIGIYRGLLRRTAAGTEGDYLVLEYVGGDRLFVPVEK
                     MSRVQRYVASEEGRAQLSRLGGTAWQRAKRKVRDDLLAMAQELVDLQAKRQLAEKAPV
                     APPDAVFREFEAAFPHEETPDQEQVIREVLSDLSSPRPMDRLVCGDVGYGKT"
     gene            complement(2067..2996)
                     /gene="nadA"
                     /locus_tag="VF357_09810"
     CDS             complement(2067..2996)
                     /gene="nadA"
                     /locus_tag="VF357_09810"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX7524487.1"
                     /translation="MRKELPALKAEIGKLLKDRNAILLAHNYQRDEVQEIADITGDSL
                     GLSQEAAKTTADVIVFCGVHFMAESAAILSQGKTVLLPREDAGCPMADMITGSDLAAY
                     REAHPGAVVVTYVNSSAEVKALSDVCCTSGNAVNVVKSIPAGKEIFMVPDRNLAQWVA
                     KVSGRPLTWWDGYCPTHERLTVDATVKAKEAHPDAVLVVHPECSPEVVAMADAVLSTA
                     GMYAWCRRSPAKEFIVGTEMGILYRLRKENPGKRFHIASRALLCPNMKLTTLEDVRDA
                     LVSLSPVITVPDAVRVRAKAALDAMLAVPRDAA"
     gene            complement(3004..4020)
                     /locus_tag="VF357_09815"
     CDS             complement(3004..4020)
                     /locus_tag="VF357_09815"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX7524488.1"
                     /translation="MSRERKESVAVVGAGSWGTAFATMLAGRHESVTLWAHETEVCAD
                     LRDRRENRAFLPGIALPPAIRPTNDFAEAVSGKTVVIFAVPSHHLREVAARTAAHLSP
                     DACLVSLAKGVENGTLMRMTEVLAEASPEHAPRVAALSGPTFAREVAEGKPTGATVAA
                     HDLSIAWRLQHALSGSRFRLYADDDVTGIEIGGALKNVMAIAAGMADGLGFGHNARAL
                     LISRGLAEISRLGVRLGAHPQTFAGLAGMGDLVLTCTGDLSRNRTVGMRVGRGERIGD
                     VLSGMAMVAEGVRTAVSAVELSRRTGVPMPISEQVHGILHEGKDVREAVSELFSRALK
                     REKE"
     gene            complement(4046..4846)
                     /locus_tag="VF357_09820"
     CDS             complement(4046..4846)
                     /locus_tag="VF357_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7524489.1"
                     /translation="MAFGGSAGAWRIGLLCGALLLAGACADPAQERFERAEKELLSQK
                     MEAALADYRFIARDHPQSRYAPAALLRQGELYGGYYRNFPASLEAYESLVYNYPRAPE
                     APRALLRTAEIHLLQYLDPSSAAADLERIRKEFPRFEGEDEVLFLLARAYGAAGEDER
                     QAAALAELTGRFPNSNRAAEGRWMLAYALLGQRRYADAEREFRKLLYLSTERESTVQA
                     RWGVAQALEGMGDLQGAIAQYEALREDAGDPAYVTEKIERLKARLRKP"
     gene            complement(4850..>5642)
                     /locus_tag="VF357_09825"
     CDS             complement(4850..>5642)
                     /locus_tag="VF357_09825"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX7524490.1"
                     /translation="FIASMHTYVLFFSDQGKVYWLKVHELPEAGRASKGRAIVNLLSL
                     SPGEELASILPVREFTEGKFVMTVTARGVIKKTDLMEYSRPRAGGIISMGLNEGDRLI
                     ATALTTGQDEVFLSTRRGMSIRFHEEDVRPMGRAAVGVRGIDLEEGALVVGMEILKPE
                     GTLLSVTEHGYGKRTAVEEYRSQSRGGKGVITLKVTGKTGHVVGVSQVAETDEVMLVT
                     DGGKIIRLGMGDLRVIGRNTQGVRLIGLAEKERVVSIARLAEKEE"
BASE COUNT          844 a         2117 c         1835 g          846 t
ORIGIN      
        1 cggtctttcc gtaccccacg tcgccgcaga cgagccggtc catcgggcgg ggggaggaga
       61 ggtcggacag cacctcgcgg atcacctgct cctggtccgg cgtctcctcg tgggggaacg
      121 ccgcctcgaa ttcccggaag accgcgtcgg gcggcgcaac gggggccttc tcggccagct
      181 ggcgcttcgc ctgcaggtcc accagctcct gggccatcgc gagcaggtcg tcccgcacct
      241 tccgcttggc gcgctgccac gccgtccccc ccaggcgcga aagctgcgcg cgtccctcct
      301 ccgacgccac gtaccgctgc accctggaca tcttctccac ggggacgaac agccggtcgc
      361 cccctacgta ctcgaggacg aggtagtccc cctccgtccc ggcggccgtc cggcgcagca
      421 ggccgcggta gatcccgatg ccgtgatcca cgtgaaccgc cgggtcgttc acccgcaagt
      481 ctgcgagcga aaactcctcg gggacggcga tccgctcctt ccgcgggcga cgcgcccggg
      541 ccttctcccc gaagatctcg ctctccgtga cgatcgccac gcgaagctcc ggcgcccgga
      601 acccccgcgt gacttccgac ccgcacagga agaccccgcg atccctggac agcgcctcgc
      661 gcagcgtgtc gacgcgggag agcggaacgg cgtagcggga gaggaaatcc tccatccgat
      721 ccacttgtga cggggagagg gagctgacga tgagccggtc tccccgcttc caccacacct
      781 tcgcctcctc ggccagcggg tagaggagcc cttccgacga ggcggtggcg gtgcttcggc
      841 ggatctcctc gttcccgtcc atccccgcgt ccccccgaag cggctccttc cgcccgaagg
      901 ggggaacctc gatcccgtcg aaggcgagca tcgggacgcc ggagagcgcg gcgaccagct
      961 cggcttccgg cacgaccaac tccgacggcg cggggtaccc ctcttcctcc ctcacgaggg
     1021 cgtacatctc ctccgcctcc tcgaaggtgt tccgggcggc ggcgacgcac tccacggaat
     1081 cgaccgccac gacgagagca ttcgcgggaa ggtacgaaaa gaccggggcg gcgtggccgt
     1141 agaggcgcgg caggagcgcg tcggcgccgt ggaaccggat cccttgccga agagtctcct
     1201 tccatgggaa cgaccctgag gcacgccccc ccttgggggc ctgctccatg ccgacgggcc
     1261 cgccgacatc ctcggcgggc ccgtcgcacg ccgccgacag gcagtcgtcc cgcgtgatca
     1321 cctgcgagca cggaaggatg acgagccgtt ccccgtcccc gggctccccg tccgcccgcg
     1381 ctcccgccgc caccgtgcgc tgcgtcgccg ggtggaacca ccgcaccgac tcgacgacgt
     1441 cgccgtccag caggaggcgc gccgggagcg gatgagccgg gctgtacacg tcgacgatcc
     1501 ccccccgcac cgcgaaatcc cccgggtcgg ctgccgcggg aaggcgggcg tagccgaggg
     1561 tgaccaggcg cgccgcgaac gcgtcgacgt cgagcgtgtc ccccgtggat accgcgccga
     1621 ccgcgtcgac gaagacctcg ggggggagcg tcttctccac cgcggcggcg atcggggcga
     1681 cgaccacggc ggggggtccg gataggagac ggtgcagcgc acgcatccgg tcgtggaccg
     1741 atgggaggtg cggagggacc ggctcatacg gcgtcgcctc gatggaaggg aacgggagga
     1801 ccgtgtcgga gccaagatag gcggtcagtt cccgggtcgc ctcctccgcc tccttctcgc
     1861 cggcgcacag gtaaaggagc gtgcggtcga ggcgctcgaa gagacgggcc gcgaggaacg
     1921 accgggcacc gggagggatc cgcatccact ggacgcaggg gggggtcccg cgcttcgcga
     1981 gggcaagggc gacgtgctcg aaggagagtc tggggagggt gtggggcatt ctgcgaatat
     2041 ggagggccgg tggcgcctgc gcaaacttac gcggcgtcgc ggggaacagc gagcatcgcg
     2101 tcgagggccg ccttcgcgcg gacccgcacc gcatcgggca ccgtgatgac cggcgagagg
     2161 gagacgagcg cgtcccgcac gtcttcgagc gtcgtgagct tcatgttcgg gcacagaagg
     2221 gcgcgcgagg cgatgtggaa ccgcttcccc gggttctcct tccggagccg gtagaggatt
     2281 cccatctccg tgccgacgat gaactccttc gcgggggagc gtcggcacca ggcgtacatt
     2341 cccgccgtgg agaggaccgc atccgccatc gccaccacct cgggcgaaca ttcgggatgg
     2401 accacgagca cggcgtctgg gtgggcctcc ttcgccttca ccgtggcgtc gacggtcagc
     2461 cgctcgtggg tcgggcaata gccgtcccac cacgtgagcg ggcggcccga caccttcgcc
     2521 acccactgcg cgaggttccg gtcggggacc atgaagatct ccttccccgc cgggatcgac
     2581 ttcaccacgt tcaccgcgtt gcccgaggtg cagcagacat cggagagggc cttcacctcc
     2641 gcggaggagt tgacgtacgt cacgacgacg gcgccggggt gcgcttcccg gtacgccgcg
     2701 aggtcggacc ccgtaatcat gtcggccatc gggcaccccg cgtcctcgcg cggcaagagg
     2761 acggtcttcc cctgcgacag gatcgcggcg ctctcggcca tgaaatggac gccgcagaag
     2821 acgatcacgt ccgccgtcgt cttcgccgcc tcctgcgaga gccccagcga gtcgcccgtg
     2881 atatccgcga tctcctgaac ctcgtcccgc tggtagttgt gcgccagcag gatcgcgttc
     2941 cgatccttca gcagtttccc gatctccgcc ttgagggctg gcagttcctt ccgcatcgcg
     3001 ttcctactcc ttctcccgct tcagcgcccg ggagaagagc tcgctcaccg cttcccggac
     3061 gtctttcccc tcgtggagaa tgccgtgcac ctgctcagag atcggcatcg gcacccccgt
     3121 gcggcgcgac agttccacgg cggaaaccgc cgtccggacc ccctcggcca ccatcgccat
     3181 ccccgagagg acgtcgccga tcctctcccc ccgccccacg cgcatcccca cggtccggtt
     3241 ccgcgagagg tcgcccgtgc aggtgaggac aaggtccccc atccccgcaa gccccgcgaa
     3301 cgtctggggg tgcgccccga ggcggacgcc cagccgggag atctcggcga gtccccggga
     3361 gatgaggagc gcccgcgcgt tgtggccgaa ccccaacccg tcggccatcc ccgcggcgat
     3421 cgccatcacg tttttcagcg ccccgccgat ctcgatcccc gtcacgtcgt cgtccgcgta
     3481 gaggcggaag cgggaaccgg acagcgcgtg ctgcagccgc cacgcgatgg agaggtcgtg
     3541 cgccgccacc gtagcccccg tcggcttccc ttccgccacc tcgcgtgcga aagtcgggcc
     3601 ggaaagagcc gcgacacggg gggcgtgttc cggggacgct tccgcgagca cctccgtcat
     3661 tcgcatcaat gtcccgttct ccaccccctt ggccaaggag acgaggcacg cgtcggggga
     3721 caggtgcgct gccgtccgcg ccgccacctc ccgcaggtga tgggacggaa cggcgaagat
     3781 caccaccgtc ttcccggaaa ccgcctccgc gaaatcgttc gtcggccgga tcgccggggg
     3841 aagggcgatc cccggcagga acgcccggtt ttcccgccga tcccggaggt cggcgcacac
     3901 ctccgtctcg tgcgcccaga gagtgaccga ttcgtgccgc ccggcgagca tcgttgcgaa
     3961 cgccgtcccc cacgaccccg ccccgacgac ggcgaccgac tccttccttt cccggctcac
     4021 cgcctcccgt cccttcccgg ggccttcacg gcttccggag acgggccttc agccgctcga
     4081 tcttctccgt tacgtacgcc gggtcgcccg cgtcttcccg cagcgcttcg tactgggcga
     4141 tcgcgccctg gaggtccccc atcccctcga gcgcctgggc caccccccag cgcgcctgga
     4201 ccgtggattc gcgctccgtc gaaagataca ggagcttgcg gaactcccgt tccgcgtcgg
     4261 cataccggcg ctgcccgagg agcgcgtacg caagcatcca tcgcccctcc gccgcccggt
     4321 tcgaattggg aaaccgcccg gtcagttcgg cgagcgccgc cgcctgccgc tcgtcttccc
     4381 ccgcggcgcc gtacgcccgc gcgaggagga agagcacctc gtcctccccc tcgaaccgcg
     4441 ggaactcctt ccggatccgt tccagatccg cggccgccga ggaggggtcg aggtactgca
     4501 gcaggtggat ctccgcggtc cgcagcagcg cgcgcggcgc ctcgggcgcc ctcggatagt
     4561 tgtacacgag ggattcgtac gcctcgagcg acgcggggaa gttccggtag tagccgccgt
     4621 agagttcccc ctggcgcagc agggcggccg gggcatacct cgactgcgga tggtcccggg
     4681 cgatgaaccg gtagtcggcg agggctgcct ccatcttctg cgaaaggagc tccttctccg
     4741 cccgttcgaa ccgctcctgg gccgggtcgg cgcacgcccc cgcaagaagc agcgccccgc
     4801 acaggagccc gatcctccac gctcccgccg atccgccgaa ggccatcgct cactcttcct
     4861 tctccgccag ccgcgcgatg gagacgaccc gctctttctc cgcgaggccg atcaggcgga
     4921 cgccctgcgt gttcctcccg atcacccgca gatcgcccat cccgagccgg atgatctttc
     4981 cgccgtcggt gaccagcatg acctcgtccg tttcggcgac ctggctgact ccgaccacgt
     5041 gaccggtctt ccccgtcacc ttcagggtga tcacgccctt gccgccccgc gattggctgc
     5101 ggtactcctc gaccgccgtg cgcttcccgt acccgtgctc cgtcacggag aggagcgttc
     5161 cctcgggttt gaggatctcc atccccacga cgagggcccc ctcctcgagg tcgatgccgc
     5221 gcacgcccac cgccgcgcgc cccatcggcc ggacgtcctc ctcgtggaac cggatcgaca
     5281 tcccccggcg ggtggagagg aacacctcgt cctgccccgt ggtgagggcg gtggcgatca
     5341 gccggtcccc ctcgttcagc cccatggaga tgatcccgcc cgcccgcggc cgggaatatt
     5401 ccatcagatc cgtctttttg atgacgcctc gcgccgtgac cgtcatcacg aacttcccct
     5461 ccgtgaactc gcggacgggg aggatcgacg cgagctcctc cccgggggag agggacagga
     5521 ggttcacgat ggcccgcccc ttcgaggcgc gccccgcctc cggcaattcg tggaccttca
     5581 gccagtacac cttcccctgg tccgagaaga agaggacgta ggtgtgcatc gaggcgatga
     5641 ag
//
LOCUS       DASVJL010000475         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_834549, whole genome shotgun sequence.
ACCESSION   DASVJL010000475 DASVJL010000000
VERSION     DASVJL010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834549"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(384..1622)
                     /locus_tag="VF357_09830"
     CDS             complement(384..1622)
                     /locus_tag="VF357_09830"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX7524491.1"
                     /translation="MRGKAYVVGLAGALLLAGCGANRAAMNGNPEHPYPPKGGPKAGE
                     IYHLPTGLAISEDGLMDMLSGARMVCVGETHDNLNDQRVELTLVRELHRRFPGKVAVG
                     MEMFREPQQAVLDRWVKGELTELEFLKASKWYETWGFDFGAYRDLLLFAKENRIDLIA
                     MNPSKDLQEAVRRTGLDNVPEDLRRMLPEIGETDPWQRVVLRGVFVGHAGHGGGDASF
                     DSFLRVQLLWEETMAQKVVDYLKSPRGEGKRMVTITGGWHVKYGFGLPKKVVRRLPMD
                     YAILLPEEISTPEQQEGRLMRVDLPEIPLLPAHFLWYVPFNSIDEKRVRMGVRMEEKE
                     GRLLVESVAPGSPASIAGIAKGDELLALDGQPVKEPVDVLFRVGGKRQGDTARVTVRR
                     GGEEKILDLTFFKMPKPKGH"
     gene            complement(1651..>1905)
                     /locus_tag="VF357_09835"
     CDS             complement(1651..>1905)
                     /locus_tag="VF357_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX7524492.1"
                     /translation="GKVDSISIREYQAVVRMKIRKGIGLPDDTIASVKTRGLIGDVYI
                     SLSPGGSNRLIPPGGRILDTEAAVDLEGLIGQFIYGSVKK"
BASE COUNT          335 a          677 c          546 g          347 t
ORIGIN      
        1 gttggcgccc atccggatga agaacgacat ggaatccccc ttgaaacgtg ggttgtgggc
       61 gagggacact cctccaaccg cagtgccgac aacctccagg aatgtccctc gaaagcgctt
      121 gcaccttata ggaaattttg cctgccgtac cctcagatat taacatcctt ctgtggcctg
      181 cgccgggcta cgtcggagct tagtgctcca atgcataaat gcggcaatgc ctctggaaga
      241 cagcgcggaa ggtgcaccag cgggagggtc cggcggagtc gccgtaggag ggggggcgaa
      301 gtgaggtaaa gcgcagccgt gcaggttcat cgcacggcga gccacgaacg gagccccgcc
      361 ctccgaggcg acgcagccgg agcttagtgc cccttcggct tcggcatctt gaaaaacgtg
      421 agatcgagga tcttctcctc cccgccgcgc cggacggtga cccgcgcggt gtctccttgg
      481 cgcttcccgc cgacccggaa gaggacatcg acgggttcct tcaccggctg cccgtcgagc
      541 gcgagcagct cgtctccctt cgcgatcccg gcgatcgaag ccggggatcc cggcgcgacg
      601 gattcgacga ggagacgccc ctccttctcc tccatccgga cccccatccg gacccgcttc
      661 tcatcgatgc tgttaaacgg gacataccag agaaaatggg ccgggagcag cgggatctcg
      721 ggaagatcca cccgcatcag ccgcccctcc tgctgctccg gcgtgctgat ctcctcgggg
      781 agcaggatcg cgtagtccat cggcaggcgg cgcaccacct tcttcgggag gccgaatccg
      841 tacttcacat gccagccgcc ggtgatcgtc accatccgct tcccctcccc ccgggggctc
      901 ttcaggtaat cgaccacctt ctgcgccatc gtctcctccc agagaagctg gacgcggagg
      961 aaggagtcga acgatgcgtc cccgccgccg tgccccgcat gcccaacgaa aacgccgcgc
     1021 aggacgaccc gctgccacgg atcggtctcg ccgatctccg ggagcatccg gcgaaggtct
     1081 tccggaacat tgtcgagccc cgtccggcgg accgcctcct gcaggtcctt cgacggattc
     1141 atggcgatca ggtcgatccg gttctccttc gcgaagagaa gcaggtcccg gtaggcgccg
     1201 aagtcgaacc cccacgtctc gtaccatttc gatgctttca ggaactcgag ctccgtaagt
     1261 tcccccttca cccaccggtc gagaaccgcc tgctgcggtt cccggaacat ctccatcccg
     1321 acggcgacct tccccgggaa gcgacgatgg agctcgcgga cgagggtcag ctcaaccctc
     1381 tggtcgttca ggttgtcgtg cgtctccccg acgcatacca tccgtgcacc ggagagcatg
     1441 tccatcaacc cgtcctcgga aatcgcaagc cccgtcggca ggtggtagat ctccccggcc
     1501 ttcggcccgc ccttcggcgg gtacggatgt tcggggttcc cgttcatcgc cgccctgttg
     1561 gcgccgcagc cggccagaag cagggccccc gccagcccga cgacgtaagc cttccctcgc
     1621 aacggcatcc ctctcccttc acccagttac ttattttttt acgcttccgt aaatgaactg
     1681 gccgatcagc ccctccaggt ccaccgccgc ctcggtatcc aggatcctgc ctcccggagg
     1741 gatcaaccgg ttcgacccgc cgggagatag gctgatgtaa acgtctccga tgagcccccg
     1801 ggttttcacc gatgcgatcg tgtcgtccgg cagcccgatc cccttccgga tcttcatccg
     1861 gaccaccgcc tggtactccc ggatcgagat cgagtcgacc tttcc
//
LOCUS       DASVJL010000476         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_83290, whole genome shotgun sequence.
ACCESSION   DASVJL010000476 DASVJL010000000
VERSION     DASVJL010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83290"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VF357_09840"
     CDS             <1..379
                     /locus_tag="VF357_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7524493.1"
                     /translation="ARRSEFAGVPVRRYRLAVFVLSGAFAGLAGSLEALLESNARPFM
                     AHWTHSAEPILVSLLGGLSSLTGPLVGSVIYIAMREVIQRFTENWMLGFGIVLLVIIM
                     GFRGGVMGTLSALFRKAEAKGEG"
     gene            386..1168
                     /locus_tag="VF357_09845"
     CDS             386..1168
                     /locus_tag="VF357_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7524494.1"
                     /translation="MDEPLLTTQKLSNHFGMVCTARDLDLTFEQGVLTSIIGPNGAGK
                     STLINLLTGHLPVQVGKIRFQGRDITRLPIHKRVRMGICRSFQIVNVFPELTVAQNVM
                     IPVLARTGRSWRPIRSLSRETAALQETEAILEKVALLPERDTPATALSHGDLRLLEVG
                     VALAAAPTLLFLDEPTAGMNPVERVRLLDNIRQLAREGRITFVVVEHDMDVVFSLSER
                     IIVLHRGEILCDGTPGQVRADPKVREVYLGDDVSDLFRYQEE"
     gene            1173..1874
                     /locus_tag="VF357_09850"
     CDS             1173..1874
                     /locus_tag="VF357_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7524495.1"
                     /translation="MILEAANVDTFYGTSHILQEVSLSVGEGEVVALLGRNGVGKTTT
                     LRSIMGLSPPKRGAIRLRGKEIAGLPSYEVARLGVAYVPDDLRIFPDLTAEENLEIAR
                     RLSRRQGYWNRERVYELFPKLADLAPAKGINLSGGEKKMLGIGRALMANPSLLLLDEP
                     SEGLAPMVVANLVEVLGRIHAQGVTILLADQNLKFCRKVCGRGYVLEKGVVRFGGRME
                     EIWGNEEVIRKYLVV"
     gene            1876..>2419
                     /locus_tag="VF357_09855"
     CDS             1876..>2419
                     /locus_tag="VF357_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7524496.1"
                     /translation="MRNVGADLFSLTGKTALVTGASKGLGRAMALGLAKAGCALALCA
                     RDAGGLRETRTAAEALAVRAETFAMDVLRRESVADAVEAVAGSFGGIDILVNNAGVNV
                     RKATLDLSEEEWDRVLDTNLKGYFLVAQAVAPYMIRQQRGKVIHISSIFGAVGMNNQL
                     AYACSKGGINQMTKVMAIEWA"
BASE COUNT          394 a          750 c          890 g          385 t
ORIGIN      
        1 cgcccggcgc agcgaattcg ccggcgtccc ggtacggcgc taccgcctgg ccgtcttcgt
       61 tctgagcggg gcgttcgcgg gtcttgccgg atcgctggaa gcgctcctgg agagcaacgc
      121 gcgccccttc atggcccact ggacccattc cgccgagccg atcctggtca gcctgctggg
      181 cgggctgtcg tcgctcacgg gccccctggt cggcagcgtg atctacatcg cgatgcgcga
      241 ggtcatccag cggttcaccg agaactggat gctggggttc gggatcgtcc tcctggtgat
      301 catcatggga ttccgcggcg gcgtcatggg gaccttgtcg gccctgttcc ggaaggccga
      361 agcgaagggg gagggataac acgggatgga cgagcccctg ctcacgacgc aaaagctgtc
      421 gaaccatttc gggatggtct gcaccgcgcg ggatctcgat ctgacgttcg agcagggggt
      481 gctgacctcc atcatcgggc cgaacggagc gggaaagtcg acgctgatca acctcctcac
      541 ggggcacctc ccggtgcagg tcgggaagat ccgcttccag gggcgggaca tcacccgcct
      601 ccccatccac aagcgggtcc gcatggggat ctgccggtcg ttccagatcg tcaacgtgtt
      661 cccggaactc accgtcgcgc agaacgtgat gatcccggtg ctggcgcgga cgggaaggtc
      721 gtggaggccg atccggagcc tctcgcggga gaccgcggcc ctgcaggaga cggaggcgat
      781 cctggaaaag gtcgccctgc ttccggaacg cgacacgccc gccaccgcgc tgtcccatgg
      841 agaccttcgc ctcctggaag tcggcgtggc gctcgccgcc gccccgacgc tgctgtttct
      901 cgacgagccc accgcgggga tgaatcccgt ggagcgggtc cgcctcctcg acaacatccg
      961 ccagctcgcg cgcgaggggc gcatcacctt cgtggtggtg gagcacgaca tggacgtggt
     1021 gttttccctc tccgagcgga tcatcgttct ccaccgcggg gagatcctct gcgacggcac
     1081 gcccgggcag gtccgggccg acccgaaggt gcgcgaagtg tacctgggcg acgacgtctc
     1141 cgatctcttc cggtatcagg aggagtgagg ccttgatcct cgaagcggcg aacgtcgaca
     1201 cgttctacgg gacgagccac atcctccagg aggtttccct ctccgtggga gaaggggagg
     1261 tggtcgcctt gctggggagg aacggcgtcg gcaagaccac gacgctgagg tcgatcatgg
     1321 ggctttcccc gccgaagcgg ggggcgatcc ggctgcgcgg gaaggagatc gccggcctcc
     1381 cgtcgtacga ggtcgcccgc ctcggcgtgg cgtacgtccc cgacgatctg cggatctttc
     1441 ccgacctcac cgcggaggag aacctggaga tcgcgcgcag gctctcccgg cggcaggggt
     1501 actggaaccg ggagcgggtg tacgaactgt tcccgaagct ggcggacctc gcccccgcga
     1561 aggggatcaa cctgtcgggc ggggagaaaa aaatgctggg gatcggccgg gcgctgatgg
     1621 cgaacccttc gctgctcctg ctcgacgagc cgtccgaggg gctcgcgccg atggtggtgg
     1681 cgaacctggt ggaggtgctc ggccggatcc acgcgcaggg ggtgacgatc ctgctcgccg
     1741 accagaacct gaaattctgc cggaaggtgt gcggccgggg gtacgtcctc gagaaggggg
     1801 tggtccggtt cggagggagg atggaggaga tctgggggaa cgaagaggtc atccggaaat
     1861 acctggtggt gtgaggtgcg caacgtgggc gcggacctgt tcagcctgac ggggaagacg
     1921 gcgctggtca ccggcgcctc gaaggggctg gggcgcgcga tggcgttggg gctggcgaag
     1981 gccgggtgcg ccctggcgct gtgcgcccgg gacgcggggg ggcttcggga aacccgaacg
     2041 gcggcggagg cgctggcggt ccgggcggag acgttcgcaa tggacgttct gcgacgggag
     2101 agcgtggcgg acgcggtcga agcggtcgcc ggctcgttcg gcgggatcga catcctggtg
     2161 aacaacgccg gggtgaacgt ccgcaaggcc accctcgacc tgtcggagga ggagtgggac
     2221 cgcgtcctcg acacgaacct gaaggggtac ttcctcgtcg cgcaggccgt ggccccctac
     2281 atgatccggc aacagcgggg gaaggtcatc cacatctcct cgatcttcgg cgccgtgggg
     2341 atgaacaacc agctcgccta cgcctgcagc aagggcggca tcaaccagat gacgaaggtg
     2401 atggcgatcg agtgggcgc
//
LOCUS       DASVJL010000477         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_459329, whole genome shotgun sequence.
ACCESSION   DASVJL010000477 DASVJL010000000
VERSION     DASVJL010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459329"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VF357_09860"
     CDS             complement(<1..714)
                     /locus_tag="VF357_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524497.1"
                     /translation="MTEPTATPDDLYLQRVTQAVIEFAKGIKSASFYPAGHPVLLQAV
                     TKIIQLFEAIPLPEQGLSIDVTKNALLYRDVPLPTGGNKAIMDLNRELYLRRASRIIF
                     LPNLQPDEVVSCLKVITLDPDEIQDAGGLERILLQEKVTRIWANRVDYDQLTQLLKEE
                     EFEEVQPEELTDDPLASSDNQRQEDVPPEEVVTIDTLIARIAKETDPSAYRGHIVEFS
                     RFLLAERAERKIEFSMQAMA"
     gene            820..1254
                     /locus_tag="VF357_09865"
     CDS             820..1254
                     /locus_tag="VF357_09865"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX7524498.1"
                     /translation="MRAVATGFGAGRVPVAPGTAGTLVALPLWYWSGGWGLRHLLLLC
                     AVLLVSVPAAREEMAATGSPDPRSVVIDEIAGMLLAATGIPWGVRPVLLLFLLFRVFD
                     VFKFGPTAWLDARRGAIYVVADDLAAGVCAGLAYRGITWLTG"
     gene            1239..>1560
                     /locus_tag="VF357_09870"
     CDS             1239..>1560
                     /locus_tag="VF357_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEX7524499.1"
                     /translation="MADRLTASSAKLLGAVLSASGRTLAVAESCSGGLLGGAITSIPG
                     SSLYFSGGVVAYGNSAKISLLGVPPDRIATHGAVSREVALAMAEGVLSLFRADLAIAV
                     TGVAG"
BASE COUNT          220 a          469 c          540 g          331 t
ORIGIN      
        1 cgccatcgcc tgcatcgaga attcgatctt ccgctcggcg cgttccgcga ggaggaaccg
       61 cgagaactca acgatgtgcc cgcggtaggc ggaggggtcg gtctccttcg cgatccgtgc
      121 gatcaaggta tcgatcgtaa cgacttcctc gggcggcaca tcctcctgcc gttgattgtc
      181 cgaggacgcc agcgggtcat cggtgagttc ctcgggctgg acctcctcga attcctcctc
      241 cttcaggagt tgcgtgagct ggtcgtagtc cacccggttt gcccagatcc gcgtcacttt
      301 ctcctgcagc aggatccgct ccagcccccc ggcgtcctgg atttcatccg gatccagcgt
      361 gatgaccttg aggcacgaga cgacctcgtc cggctggagg ttgggaagaa agatgatccg
      421 tgacgcgcgc cgcaggtaca gctcccggtt gaggtccatg atcgccttgt tcccgccggt
      481 gggcagcggg acgtcccggt agaggagggc gttcttcgtg acgtcgatcg aaaggccctg
      541 ttcgggcagg gggatcgcct cgaaaagctg gatgatcttc gtgacggctt gcagcaggac
      601 agggtggcct gcgggataaa agctcgcgct tttgatcccc ttggcgaatt cgatgaccgc
      661 ctgcgtgacc cgctgcaggt aaaggtcgtc cggggttgcc gtgggctcgg tcattccatc
      721 gttggaaaat gaggttttat tgacatacag tagtaaatat ccgacatggg cgctgtacaa
      781 gtcaatcgtt ttcgggaaaa ccgatgggtt cgcggagcgt tgcgcgccgt cgccacgggg
      841 ttcggtgcgg gcagggttcc cgtggccccc gggacggcgg ggaccctggt cgccctgccc
      901 ctctggtact ggtcgggcgg atgggggttg cgtcatcttc tcctcttgtg cgcggtgctc
      961 ctcgtttccg tcccagccgc ccgggaggag atggccgcga cggggagtcc ggatccaagg
     1021 tccgtggtca tcgacgagat cgcggggatg ctgttggcgg ccaccggcat cccctggggg
     1081 gttcgccctg tcctcctgct cttcctgttg ttccgtgttt tcgatgtgtt caaattcggg
     1141 cccacggcat ggctcgacgc ccgcaggggg gccatctacg tggtggcgga cgacctggcg
     1201 gcaggggtgt gcgcgggact cgcgtaccgg gggatcactt ggctgaccgg ttgaccgcgt
     1261 cgtcggcgaa gctccttgga gcggtgttgt ccgcctccgg gaggaccctc gccgtggcgg
     1321 aatcgtgctc cgggggcctg ttgggaggag cgatcacttc gatccccggc agttccctct
     1381 atttttccgg cggggtcgtg gcgtacggca attccgcgaa gatctcactg ttgggcgttc
     1441 cgcccgaccg gatcgccacg cacggagcgg tgagccgcga ggtggcgctt gcgatggcgg
     1501 aaggggtcct ctccctcttc cgggcggacc ttgcgatcgc cgtcaccggc gtggcggggc
//
LOCUS       DASVJL010000478         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1398500, whole genome shotgun sequence.
ACCESSION   DASVJL010000478 DASVJL010000000
VERSION     DASVJL010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398500"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VF357_09875"
     CDS             <1..237
                     /locus_tag="VF357_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524500.1"
                     /translation="IATRTDRMGKGYGRSILSFVEEEVRRRGGYLLVIETSSQESYGS
                     TRQFYDKVGCTLAAQLPDYYAKGDDKLIYLKRVG"
     gene            complement(254..>1572)
                     /locus_tag="VF357_09880"
     CDS             complement(254..>1572)
                     /locus_tag="VF357_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX7524501.1"
                     /translation="RGRASLPDLLATLYGETGVDFVAARIPDGERIVRNLSKAAEMAR
                     SFEWGGAGSLNLFLAEIRRKADEGREEDEVPDFEEGEDAVRLSTIHGAKGLEFPVVIL
                     GGISRGDRKGPEGLRADRVRGLSAVIFPGFRTYSAFRQVPGAGRPVTFEEWEKEKTVG
                     EERRLLYVAATRAKDRLYLVDGGKGAGSTLRDALREGTSGATAAGEGRCLVTGLPGER
                     LRLAESGAGGPVGGELLRVSVTSALREEARHPPAPLVVQAISGERAASVAEPAPPAPT
                     PEPVSLADLHDRSRGKRFGEKVHRVFEAYPPVTDPWPPVGADLPVIWDEEEERRWNAI
                     VAAVRSSPLRRELLSTLVVGTELPLLRVRGFTANEERADLVVRAPGPAGELRVIDYKT
                     GERDRPLEEAYFLQLREYRDILSEAWRVPVRGILWYVETGASFEVT"
BASE COUNT          252 a          599 c          506 g          215 t
ORIGIN      
        1 atcgcgacgc ggacggaccg gatgggaaaa gggtacggac ggtccatcct gtcgttcgtc
       61 gaggaggagg tccggcggcg cggaggatac ctcctggtga tcgagacgtc gtcgcaagag
      121 agctacggct ccacgcggca attctacgac aaggtcggct gcaccctcgc cgcgcagctg
      181 cccgactact acgcgaaggg agacgacaag ctcatctacc tgaagcgggt cggctgaggg
      241 caggttacga cgatcacgtc acctcgaacg acgcgccggt ttccacgtac cacaggatcc
      301 cccgcacggg gacgcgccac gcctcggaga ggatgtcgcg atactcccgc agctggagga
      361 agtacgcctc ctcgagcggc cgatcacgct ccccggtttt gtaatcgatg acccgcagct
      421 cccccgccgg tcctggggcg cgaacgacga ggtcggcgcg ctcctcgttc gcagtgaagc
      481 cgcggacccg caggagcggc agttccgtgc cgacgacgag ggtggagaga agctccctgc
      541 gcaggggaga ggagcgtacg gcggcgacga tcgcgttcca ccgacgttcc tcttcctcat
      601 cccagatcac cggaaggtcc gcccccaccg gcggccacgg atcggtcacc ggcgggtacg
      661 cctcgaagac gcggtggacc ttctccccga accgctttcc gcgcgaccgg tcgtgcaggt
      721 ccgcgaggga gaccggctcg ggtgtcggcg cgggcggggc cggctccgcg accgaagcgg
      781 cccgttcccc tgagatcgcc tgcacgacga ggggcgcggg gggatgccgg gcctcctccc
      841 gcaaggccga cgtcaccgac acgcggagca gttcccctcc caccggtccg cccgcgccgg
      901 attccgccaa ccgcaaccgc tcgcccggaa gccccgtgac gaggcagcgg ccctcccctg
      961 ccgccgtcgc gccggaggtc ccttcccgca gcgcgtcccg aagggtcgag ccggccccct
     1021 tccccccgtc caccagatag agccgatcct tcgcgcgggt ggccgcgacg tagagaaggc
     1081 ggcgctcctc ccccaccgtc ttctccttct cccactcttc gaacgtcacc gggcggcccg
     1141 cgcccggcac ctgccggaac gccgaatacg tccggaaccc ggggaagatc accgcggaaa
     1201 ggccccgcac ccggtccgcc cgcaaccctt ccggcccctt ccggtcgccg cgggagatcc
     1261 ccccgaggat cacgaccggg aactcgagcc ccttcgcccc gtggatcgtg gagaggcgca
     1321 cggcgtcctc cccttcctcg aagtcgggga cctcgtcctc ctcccgaccc tcgtcggcct
     1381 tgcggcggat ctccgcgagg aagagattga gggacccggc gcctccccat tcgaaggacc
     1441 tggccatctc cgcggccttc gacaggttcc ggacgatccg ttccccatcg ggaatccgtg
     1501 ccgcgacgaa atcgaccccc gtctccccgt acaaggtcgc gagaaggtcc gggagcgacg
     1561 cccgaccccg ct
//
LOCUS       DASVJL010000479         3724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1679122, whole genome shotgun sequence.
ACCESSION   DASVJL010000479 DASVJL010000000
VERSION     DASVJL010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679122"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VF357_09885"
     CDS             complement(<1..693)
                     /locus_tag="VF357_09885"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7524502.1"
                     /translation="MDAQIRIRNAGKVFRAHGKEVEALRDISFDIPNGQFVCLLGPSG
                     CGKSTLLNAVAGFSLPTSGSVEVEGRPVEGPGPDRGMVFQEYAIFPWMTVEQNVAFGL
                     EIRKRPAAEIREKVSSLLAMLSLSDFRDRYPKDLSGGMRQRVAIARVLALDSRILLMD
                     EPFGALDALTRHNLQDELLRIWEAFRKTVLFVTHSIEESIFLADRILVMTYRPGTIKR
                     DIAVTLPRPRDPG"
     gene            complement(725..1561)
                     /locus_tag="VF357_09890"
     CDS             complement(725..1561)
                     /locus_tag="VF357_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7524503.1"
                     /translation="MTSGNGGTEKVLGFLRGAAVPAVLIAVWQLLSMMGYMNPQILPA
                     PSAVLVRWYQYLAPIDAYDPAKSGYVAWLLSGELVADAYASLSRVVVGFLIGAGLALP
                     LGLAMGATERVYKLFDPLVQVLRPIPPIAYIPLSILWFGLGNPPAIFLISIGAFFPVL
                     MNTISGVRHVDGIYIRAARNLGAGRATMFLRVILPAATPYILTGARIGVGTAFIVVIV
                     SEMIAVNNGLGFRILEAREFFWSDKIIAGMFSIGLLGLGIDTGMERLNAHLLKWHRGI
                     EN"
     gene            complement(1578..2549)
                     /locus_tag="VF357_09895"
     CDS             complement(1578..2549)
                     /locus_tag="VF357_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7524504.1"
                     /translation="MRHLGLLVAAALVACLGTVAGAEDLVRLGNLKFAHYGAVSYMKE
                     IAPKYNLRIEERVFAKGLDIVPAIIAGEIDIAASAADAAIAGRAGGAPVFAVAGFAKG
                     GARLVVRPDLGIKAVKDLKGKKVGVARGGAQELLLVAELAKHGLTWTNGPGKDVQIVY
                     MAFADLNQALMQKDIDAMCQSEPQSSQAVNKGFGVELLKPYDTPLGEPVRVLVMTEKM
                     YREKRGVAERVLKCFVEATKAFIDNPKLAEKYVRETMFKSQITSNDYKDAIANSPFTY
                     DLTVEHMQITTDLMQKYGIGRMTSPPAARDWVKLDLLEKAKKELKIR"
     gene            complement(2625..>3724)
                     /locus_tag="VF357_09900"
     CDS             complement(2625..>3724)
                     /locus_tag="VF357_09900"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX7524505.1"
                     /translation="VVKFAEIAMREMGVDIRTDPFSVKFTGGPNGDVAGNAMRILLAR
                     CPKAAIRLILDGTGALYDPAGIDRGELSRIVLRGDVEAFRPEKLRTGGFLLYRNERRT
                     EGLRELYKRMDRTEAGVVPSWVTTDEFNKEIDGLLFTVRADLFLPAGGRPETVDATNW
                     RRFFGVDGAPTARVIVEGANSFLTPEARTELQKRGIVILRDASANKCGVISSSYEIIG
                     NLLMTGKEFLAHKEEYVRDVLAILEKRVEDEALLIFRRKREAGASLSYTEISDAISVE
                     INGHYARLFDFFRSRPALAQSPPYRRALLSHLPRFLSENAKFRRRIPRLPVKYRWAIL
                     AVEIASIIVYRGGFERDLEADLKWYVRKEFG"
BASE COUNT          626 a         1265 c         1180 g          653 t
ORIGIN      
        1 ccccggatcc cgggggcggg ggagggtcac cgcgatgtcc cgcttgatcg tcccgggccg
       61 atacgtcatc accaggatcc ggtcggccag gaagatcgac tcctcgatgc tgtgggtgac
      121 gaagaggacg gtcttccgga acgcctccca gatccggaga agttcgtcct ggaggttgtg
      181 gcgggtcagg gcgtcgaggg ccccgaacgg ctcgtccatc aggagaatcc gggagtcgag
      241 ggccagcacc cgggcgatcg ccacgcgctg gcgcatcccg cccgaaaggt ccttggggta
      301 tcggtcccgg aaatcggaca ggctcagcat ggcgagcagg gaggaaacct tctcccggat
      361 ctccgccgcc ggcctcttcc ggatttccag cccgaaggcg acgttctgct ccaccgtcat
      421 ccacgggaag atggcgtact cctgaaatac cattccacgg tccggtccag gtccttcgac
      481 cggtctgcct tccacctcca ccgagcccga cgtcgggagg gagaagccgg cgacggcgtt
      541 gagcagcgtc gactttccgc acccggaagg gccgaggagg cagacgaact ggccgttcgg
      601 gatgtcgaag ctgatgtccc gcagcgcctc gacctccttg ccgtgggcgc ggaacacctt
      661 tcccgcattg cggatccgga tctgcgcgtc caaacgctgc cgcctccctg cgtcggtgct
      721 cccgctagtt ctcgatcccc cggtgccact tcaggagatg ggcgttgagc cgttccatcc
      781 cggtgtcgat ccccaacccg aggagcccga tcgaaaacat ccccgcgatg atcttgtccg
      841 accagaaaaa ctcgcgcgct tccaggatcc ggaacccgag gccgttgttc acggcgatca
      901 tctcggaaac gataaccacg atgaacgcgg ttcccacccc gatgcgcgca ccggtcagta
      961 tgtacggtgt cgccgcgggg aggatgaccc ggaggaacat cgtcgcccgg ccggccccca
     1021 ggttcctggc cgcccggatg tagatgccgt ccacgtgtcg cactccggag atcgtgttca
     1081 tgaggaccgg gaagaacgcc ccgatgctga tgaggaagat ggccggcggg ttccccaggc
     1141 cgaaccagag gatcgacagc gggatgtagg cgatgggcgg gatcgggcgg aggacctgca
     1201 cgagcgggtc gaacaatttg tagaccctct cggtcgcccc catcgccagt cccaggggga
     1261 gagcgagccc cgctccgatc aggaacccga ccaccacccg ggagaggctc gcataggcgt
     1321 cggcgaccag ttcccccgac aggagccagg cgacgtaccc cgatttcgcc gggtcgtacg
     1381 cgtcgatcgg cgccaggtac tggtaccagc ggaccagcac ggccgaaggc gcgggaagga
     1441 tctgggggtt catgtacccc atcatcgaaa ggagctgcca caccgcgatg aggaccgcgg
     1501 ggaccgcggc tccccggagg aaaccgagaa ccttttccgt tcccccgttc ccggatgtca
     1561 actgcgccgg ttccccctca ccggatcttc agctccttct tcgccttctc gagcaggtcc
     1621 agcttcaccc agtccctggc cgccggcggg ctggtcatcc gcccgatgcc gtacttctgc
     1681 atcaggtccg tggtgatctg catgtgttcg accgtcaggt cgtaggtgaa gggggagttg
     1741 gcgatcgcat ccttgtagtc gttgctggtg atctggctct tgaacatcgt ctcgcgcacg
     1801 tatttctccg cgagcttcgg gttgtcgatg aaggccttcg tggcctcgac gaagcacttg
     1861 agcaccctct cggcgactcc ccgcttctcc cggtacatct tctccgtcat cacgaggacc
     1921 cggaccggtt cgcccagcgg cgtgtcgtac ggcttcagga gctcgacccc gaaccccttg
     1981 ttgaccgcct gggacgattg cggctcggac tggcacatcg cgtcgatgtc cttctgcatc
     2041 agcgcctggt tcaggtcggc gaatgccatg tagacgatct ggacgtcttt gccgggaccg
     2101 ttggtccacg tgaggccgtg cttggccagc tcggccacca gcagcagctc ctgggccccg
     2161 ccccgggcca ctcccacctt cttccccttg aggtccttca ccgccttgat gccgaggtca
     2221 ggtcgcacca cgaggcgggc accgcctttc gcaaaccccg ccacggcgaa gacgggggct
     2281 ccgccggcgc gtccggcgat cgccgcgtcg gccgcgctcg cggcgatgtc gatctctccg
     2341 gcgatgatgg cggggacgat gtccagcccc ttggcgaaca cgcgttcctc gatcctgagg
     2401 ttgtacttcg gggcgatctc cttcatgtag gagacggccc catagtgcgc aaacttcaga
     2461 ttgccgagcc ggacgaggtc ctccgcgccg gccaccgtcc ccagacacgc gacgagcgcc
     2521 gccgcgacga gcaatcccag gtgccgcatg gatccctcct tgaggggaaa gaatgagacg
     2581 attatcgcag atccctactt ccgcggacgc ggggaatcgg gatgctaccc gaattccttc
     2641 cggacgtacc atttgaggtc cgcttccagg tccctctcga agcctccgcg gtagacgatg
     2701 atcgatgcga tctcgaccgc caggatcgcc caccggtact tgacggggag ccggggaatc
     2761 cgacgacgga acttggcgtt ctcgctcagg aaccgcggca ggtgcgacag gagggcgcgg
     2821 cggtacggag gcgactgcgc caacgcgggc cgcgaccgga aaaaatcgaa cagccgtgcg
     2881 tagtggccgt tgatctccac gctgatcgcg tcggagatct ccgtgtacga cagcgacgct
     2941 cccgcttccc gctttcgccg gaagatgaga agcgcttcgt cctctacccg tttttcgagg
     3001 atcgccagca cgtcccggac gtactcctcc ttgtgcgcga ggaactcctt gccggtcatc
     3061 agcaggttgc cgatgatctc gtaggaggag gagatgacgc cgcacttgtt cgcggaagcg
     3121 tcccgcagga tcacgatgcc ccgcttctgg agctccgtcc gggcttccgg ggtgagaaag
     3181 gagttcgccc cctcgacgat cacgcgggcc gtgggcgccc cgtccacacc gaagaagcgc
     3241 ctccagttcg tggcgtccac cgtttctgga cgcccccccg ccgggaggaa gaggtcggcg
     3301 cggacggtga agaggagccc gtcgatctcc ttgttgaact cgtccgtcgt gacccacgac
     3361 ggaacgactc ccgcctcggt ccggtccatc cgcttgtaca gttcccgcag cccttccgtg
     3421 cgccgctcgt tccggtagag gagaaatccc ccggtgcgga gcttttcggg gcggaacgcc
     3481 tcgacgtcgc cgcgaaggac gatccgggag agttcgccgc ggtcgatccc ggccggatcg
     3541 tagagggccc cggtgccgtc gaggatcaga cggatcgccg ccttcgggca cctggcaagg
     3601 aggatgcgca tcgcgttccc ggccacgtcc ccgttcggcc ccccggtgaa cttcaccgaa
     3661 aacgggtcgg tccggatgtc cacgcccatc tcgcgcatgg cgatctcggc gaatttcacc
     3721 accc
//
LOCUS       DASVJL010000480         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_931378, whole genome shotgun sequence.
ACCESSION   DASVJL010000480 DASVJL010000000
VERSION     DASVJL010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931378"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF357_09905"
     CDS             complement(<1..215)
                     /locus_tag="VF357_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524506.1"
                     /translation="MESVAAAWTLGRITGGKVATLGTGDFFRFLLFCVMAICGGSAIF
                     GSAASVFLGGNPSYLAAFFLWWAANAAG"
     gene            complement(231..524)
                     /locus_tag="VF357_09910"
     CDS             complement(231..524)
                     /locus_tag="VF357_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524507.1"
                     /translation="MDSAFRRFTAGGTSGELRKTLLVASCVAGVYYLFAKIGIFFLVH
                     PDGFAAFWPAAGLLPPVLLFLRKNDRVPTVAAVFASISIVNMTLGMPPAIGVA"
     gene            complement(613..2040)
                     /locus_tag="VF357_09915"
     CDS             complement(613..2040)
                     /locus_tag="VF357_09915"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX7524508.1"
                     /translation="MSSHVTLPVWVVAVLALLAALAAVDRLLLPGVRWFLRRRVNIVL
                     DEIGKRLHLEIRPFTLTKRQVLIDRLMYDPKVLEAAGAHATERGIPRDVVMAEVERYA
                     REIVPAFNAYAYFRMGYWLARRAARLLYRVRVGFTDEEGLSRVDPKSTVVFVMNHRSN
                     MDYILVAYLAATRTALSYAVGEWARIWPLQTLVRTMGAYFIRRDSKDPLYRLVLERYV
                     AMATEGGVVQAIYPEGGLTKDGALRTPRLGLLDYMTRAFDPKGDRDIVFIPVGINYDR
                     TLEDRSQVLALKPEAPRRGAGFAAATFFRFLLQNLLLMLQGRWYRFGYACVNFGSPLS
                     MRAYILERGIDLRALPREERFLEMEKLAAALMHSVGRVIPVLPVSLVSTALLRFPGGC
                     CSELELKASALRVIEELSGAGAHIYIPRADQDYAVTVGLRMLTLRKLVSENDGLYSPL
                     AEELPLLQYYANSIRHLIPAIAPES"
     gene            2093..>2508
                     /locus_tag="VF357_09920"
     CDS             2093..>2508
                     /locus_tag="VF357_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7524509.1"
                     /translation="MGGKPVNPRKSEAVVLIHGLWMTGTEMRVLGGRLEESGFRVRYF
                     RYHSWRGGLAQAADALREFVEATEGERVHLVGHSLGGVVIAKMLEEAPRSRPGRVAML
                     GSPMGGSATARIVSRCRVGRWLVGGVIREGIVEHAP"
BASE COUNT          477 a          765 c          864 g          402 t
ORIGIN      
        1 cccgccgcgt tcgctgccca ccagaggaag aatgccgcca gatacgatgg attcccaccg
       61 agaaacacgc ttgccgcgga accgaagatc gcggagccgc cgcagatcgc catcacgcag
      121 aaaaggagga accggaaaaa atccccggta ccgagagtgg cgaccttccc ccctgtgatg
      181 cggccgagcg tccatgcggc cgcgaccgat tccacgcaat tgaccgccgc ttaggcgacg
      241 ccgatcgcgg gcggcatccc caaggtcatg ttcacgatcg aaatggatgc gaacaccgcg
      301 gcgaccgtcg ggacacggtc gttttttcgc aggaacagca gcacgggggg caacaggccg
      361 gcagcgggcc agaaggcggc gaaaccgtcc gggtgcacca ggaagaatat tccgatcttc
      421 gcgaagagat aatagacccc cgcgacgcag gacgcgacga ggagcgtttt ccgaagttcg
      481 ccggaggtac cgcctgccgt aaatctccgg aacgctgaat ccacggcggt acctcccggt
      541 taaatatcga ctgttattca tattccctat tgttttatga agaacaaggc cgtttcggta
      601 ccggctgtga tatcaacttt ccggggcgat cgcggggata aggtggcgga tggagttcgc
      661 gtagtattgg agcaggggaa gctcttccgc aaggggagag tacagcccgt cattctccga
      721 cacgagtttc cggagggtca gcatacgcag tcccaccgtg acggcgtaat cctggtccgc
      781 gcgcgggatg tagatgtgcg cacccgcgcc ggacagttcc tcgatgaccc ggagagccga
      841 ggccttcagc tccagttcgc tgcagcatcc gccgggaaag cgcaggaggg ccgtcgatac
      901 cagggagacg gggaggaccg gaatcactcg tcccacggag tgcatcagcg ccgccgcgag
      961 cttttccatc tcaaggaacc gttcttccct ggggagagcg cggaggtcga tgccgcgttc
     1021 caggatgtac gcgcgcatcg acagcggcga cccgaagttg acgcacgcgt acccgaaccg
     1081 gtaccagcgc ccctgcagca tgaggagaag attctgcagg aggaacctga aaaaggtcgc
     1141 ggcggcgaag ccggcaccac gccgcggcgc ttccggcttg agcgcgagca cctggctgcg
     1201 atcctcgagc gtccggtcgt agttgatccc caccgggatg aagacgatgt cccggtcccc
     1261 tttcgggtcg aaggcccggg tcatgtagtc gagcaggccg agtcggggtg tgcgcagcgc
     1321 cccgtctttc gtcagcccgc cttccgggta gatcgcctgg acgacgccgc cttcggtcgc
     1381 catcgccacg tacctctcga ggacgagccg atacagcggg tccttcgagt ccctgcggat
     1441 gaagtaggcg cccatcgtcc ggacgagggt ctgcagcggc cagatccgtg cccactcgcc
     1501 gacggcgtac gacagcgccg tgcgggtggc ggcgaggtac gccacaagaa tgtaatccat
     1561 gttgctgcgg tggttcatca cgaagacgac ggtcgacttc gggtccacgc gggatagacc
     1621 ctcctcgtcg gtgaacccga cccttacccg gtatagcagc ctcgcggcgc gacgggcgag
     1681 ccagtacccc atccggaagt aggcgtaggc gttgaaggcg gggacgatct cccgggcgta
     1741 ccgttcgacc tccgccatca cgacgtctct cgggattccg cgctccgtcg cgtgcgcccc
     1801 ggccgcctcg agcactttcg ggtcgtacat caggcggtcg atgaggacct gacgtttggt
     1861 cagggtgaaa gggcggatct cgaggtgcag ccgtttgccg atctcgtcga ggacgatgtt
     1921 cacgcggcgt cgaaggaacc agcggacgcc gggaagaagc aggcgatcca cggccgcaag
     1981 ggccgccagc agggcgagga cggccacaac ccataccgga agggtcacat gtgagctcac
     2041 cggcgcagtc tatcatcaac cgatgggata taatcccggc cgggagggcg tcttgggcgg
     2101 aaaaccggtg aatccgcgga agagcgaagc ggtcgtcctg attcacggac tttggatgac
     2161 ggggacggag atgcgcgtcc tcggaggccg cctggaggag tcgggtttcc gtgtgcgcta
     2221 cttccggtac cactcctggc ggggagggct cgcgcaggcg gcggacgccc tccgggagtt
     2281 cgtcgaggcg acggaagggg agcgggtgca cctcgtcggg cacagccttg ggggcgtcgt
     2341 gatcgcgaag atgctcgagg aggccccacg atcgcggccg ggtcgcgtgg cgatgctggg
     2401 gtctccgatg ggcgggagcg cgacggcgag gatcgtgtcc cggtgtcggg tcgggcgatg
     2461 gctcgtgggc ggcgtgatcc gggaggggat cgtcgaacat gcgccgaa
//
LOCUS       DASVJL010000481         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_647444, whole genome shotgun sequence.
ACCESSION   DASVJL010000481 DASVJL010000000
VERSION     DASVJL010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647444"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..735)
                     /locus_tag="VF357_09925"
     CDS             complement(64..735)
                     /locus_tag="VF357_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7524510.1"
                     /translation="MPKDDVREHFEKIAGEYDRWKEKSAYYYRLLAGIYRERVPEGAS
                     VLEIGCGTGTLLAALSPARGVGVDISTRMVKFAAAKHPSLSFRVADAESFDSGESFDF
                     VIVPDVVEHLSDPQAMFHSARKACHAETRVIVTCVNPRWAPILHFAERLGLKMPEGEH
                     RWLPAEDLIRMAGCAGFDLVEFVGRILCPKEIPLLAHPLNRAASLLPLLRPLCLIQAL
                     VFSPR"
     gene            complement(740..>2087)
                     /locus_tag="VF357_09930"
     CDS             complement(740..>2087)
                     /locus_tag="VF357_09930"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional class I SAM-dependent
                     methyltransferase/glycosyltransferase family 2 protein"
                     /protein_id="HEX7524511.1"
                     /translation="KNRYYYEEVERIVRFHIPRGASVLEIGCGTGDLLAALAPSRGVG
                     IDISPKAVEIARSKHPGHTFLAGDAEDIPLSEPFDYILLSDVIGYINDVQSAFEQLNR
                     VCRPRTRVILTYFNYLLEPVLRAGERLGIKRPQPDQNWLSLADLQNLLSLAGFQTVSM
                     GYKVLLPVRLPLLSSLCNRVVANLPLLRKLCLVETIIARPAPVPVPEESLSCTVVIPA
                     RNEKGNIENAVRRTPEMGKHTEIVFVEGNSRDGTAEEIERVIAAYPDRDVKIVRQGSG
                     VGKGDAVRKGFAAAIGDVLMILDADLTVPPEDLPKFLKALCSGRGEFVHGSRLVYPME
                     KQAMRFLNTLGNKFFSLAFTWLLDQRFKDTLCGTKVLYRRDYERIAAGRGYFGDFDPF
                     GDFDLIFGAAKLDLKILEVPIRYRERTYGTTQISRFRHGWLLLRMCLFALRRIKLV"
BASE COUNT          376 a          691 c          686 g          334 t
ORIGIN      
        1 ccgtctaccc cttgctggtg ggaatcgcga tcgcagggac gcctgcaaac cgggaagcgg
       61 cgttcagcgc ggcgagaaca ccagcgcctg gataaggcac aggggacgca ggaggggaag
      121 aagcgaagcc gcccggttga gcgggtgggc cagcaggggg atctctttcg ggcagagaat
      181 cctcccgaca aactccacga ggtcgaaccc cgcgcaccct gccatccgga tcaaatcctc
      241 cgccgggagc caccggtgct cgccctcggg catcttcagc cccaaccgct cggcgaaatg
      301 gaggatcggc gcccagcgcg ggttgacgca ggtaacgatg acgcgcgtct ccgcgtggca
      361 cgccttccgt gcggagtgga acatcgcctg cggatcggag aggtgctcga cgacgtcggg
      421 aacgatcacg aagtcgaacg actccccgga gtcgaacgac tcggcgtccg cgacgcggaa
      481 ggagagggaa ggatgctttg cggcggcgaa cttcaccatc cgcgtggaga tgtccacgcc
      541 cacgccgcgc gcgggggaga gggcggcgag gagggtcccc gtaccgcacc cgatctcgag
      601 gaccgacgct ccttccggga cccgctcccg gtagatcccc gcgaggagcc ggtagtagta
      661 cgccgacttc tctttccacc ggtcgtactc cccggcgatt ttctcgaaat gctcccggac
      721 gtcgtccttc ggcatggggt cagacgagct tgatccgccg cagcgcgaac aggcacatgc
      781 gcaacagcag ccaaccatgg cggaaccggg agatctgtgt ggtgccgtat gtccgctccc
      841 ggtaccggat cggcacctcc aggatcttca ggtcgagctt cgccgccccg aagatcaggt
      901 cgaagtcccc gaaggggtcg aagtcgccga agtagccccg ccccgcggcg atccgctcgt
      961 aatcgcggcg gtagaggacc ttcgttccgc acagggtgtc cttgaaccgc tgatcgagca
     1021 gccaggtgaa cgcgaggctg aagaacttgt tcccgagcgt gttcaggaaa cgcatcgcct
     1081 gcttctccat ggggtagacg agccgggagc cgtggacaaa ctcgccgcgc cccgagcaga
     1141 gggccttcag gaacttcggc agatcctcgg gcggcacggt caggtcagca tcgaggatca
     1201 taaggacatc cccgatggcg gcggcgaacc ccttgcggac cgcgtcccct ttccccacgc
     1261 cgctcccctg gcggacgatc ttcacgtccc ggtccgggta cgccgcgatc acacgctcga
     1321 tctcctccgc cgtcccgtcg cgggagttcc cctcgacgaa gacgatctcg gtgtgcttcc
     1381 ccatctccgg ggttcgtcgc accgcgtttt cgatgttccc tttttcattg cgggcgggga
     1441 tcaccaccgt gcacgagagc gactcctctg gcaccgggac gggggccggt cgcgcgataa
     1501 tggtctccac caggcacagc ttgcggagca ggggcaggtt cgccaccacc cggttgcaga
     1561 gggaagacag tagcgggagg cgaacgggca ggaggacctt gtaccccatg gagaccgtct
     1621 ggaaaccggc cagcgagagg agattctgca ggtccgcgag ggagagccag ttctggtccg
     1681 gctgggggcg cttgatccca agccgttccc cagcccggag gacgggctcc aaaaggtagt
     1741 tgaagtaggt caggatcacc cgggtgcgcg ggcggcacac cctattcagt tgctcgaacg
     1801 cgctctgcac gtcgttgatg tagccgataa cgtcggagag gaggatgtag tcgaaaggct
     1861 ccgagagcgg gatgtcctcc gcgtctcccg ccaggaacgt gtggccgggg tgtttcgaac
     1921 gcgcgatctc caccgccttc gggctgatgt cgatccctac ccccctggac ggcgcgagcg
     1981 ccgcgagcag gtcccccgtc ccgcacccga tctccagcac cgacgcgccc cgcgggatgt
     2041 ggaaccggac gatcctctcc acctcctcgt agtagtaccg gttcttc
//
LOCUS       DASVJL010000482         3308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_274016, whole genome shotgun sequence.
ACCESSION   DASVJL010000482 DASVJL010000000
VERSION     DASVJL010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274016"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VF357_09935"
     CDS             complement(<1..434)
                     /locus_tag="VF357_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524512.1"
                     /translation="MWKSITGRITLIAVLTAVSIIILVPSLTDQVPAGWKDRVPKINL
                     GLDLQGGVFLRLAVEIDKAIENTSMRYADDARAACREKALPVLAFQKVADGGFSLRFP
                     PGDFATRAQATLKEEFPSLDVSLGEVKADGATVIARMKPGEI"
     gene            complement(493..837)
                     /gene="yajC"
                     /locus_tag="VF357_09940"
     CDS             complement(493..837)
                     /gene="yajC"
                     /locus_tag="VF357_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX7524513.1"
                     /translation="MTGVAYAMGGSPGGGAGGGGGLMGLLPILLMFVIFYFLLIRPQQ
                     KQASKQRDFIKNLKVGDRVVAAGGLHGEVKGLTETTVTLEIADKVRVKVTRSAVSGSS
                     QDAAVPEAQKPA"
     gene            complement(865..1974)
                     /gene="tgt"
                     /locus_tag="VF357_09945"
     CDS             complement(865..1974)
                     /gene="tgt"
                     /locus_tag="VF357_09945"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX7524514.1"
                     /translation="MTLRFAVEARDGGTAARAGTISTDRGILRTPAFMPVGTAATVKG
                     VWPDQLREMGFGCILGNTYHLYLRPGHERIRCQGGLHRFMGWDRLILTDSGGFQVFSL
                     SALRKVSDEAVTFRSHLDGSAHTLTPELAVAVQEALGSDVRMALDECVEYPAGREEVQ
                     EAVRRTTLWATRSLAARTSEGGGMFGIVQGGMFPDLRRRSVEEICALPFQGFAIGGVS
                     VGEGKEFQRETVAGTAPMLPASMPRYLMGVGTPEDILFAIARGVDLFDCVLPTRNARN
                     GMMFTSAGSVSIKQARYADDSLPPDERCDCPTCRAFSRAYLRHLYLQREMLGSMAMTM
                     HNLHFYARMMRKAREAICRGNFGELVKESVVSENE"
     gene            complement(1985..3142)
                     /gene="queA"
                     /locus_tag="VF357_09950"
     CDS             complement(1985..3142)
                     /gene="queA"
                     /locus_tag="VF357_09950"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX7524515.1"
                     /translation="MPVRGKRDGLARRGISRDPRPLLPRDRNHRRDALRTDLLEYDLP
                     ERLVAQHPPPRRRDARLMTLSRATGEIGHRVFPELPFLLRRGDLVVVNDTKVLHGRIR
                     ASRRTGGAVEVFLLSPLQEAGAAGEERWEALARPSKRLKEGEEFEVGGTLRVRLARRL
                     DEGRWEVLLSGRSVSVAKALERAGEVPLPPYIRRRPDDPASKEDAVRYQTVYARNPGS
                     VAAPTAGLHFDEDLLGELATAGVGVARLTLSVGYGTFSPIRTEEVEAYGIHPERYRLP
                     PETAAEINAARGRGGRVVAVGTTSVRTLETCAAEDGTLEPSGGTTRKFIFPGYRFRAV
                     DALVTNFHLPRSSLLALVMAFAGVDAVRAAYREAVAREYRFYSYGDAMFIS"
     gene            complement(3040..>3308)
                     /locus_tag="VF357_09955"
     CDS             complement(3040..>3308)
                     /locus_tag="VF357_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524516.1"
                     /translation="SRVRISSGGVSRELQAAEFRKAAGYAKVRSLKMEIVPVAGEWRF
                     TGEGWGHGVGLCQFGANGMAWRGAGFREILARYYPGTEIIGETP"
BASE COUNT          530 a         1227 c         1025 g          526 t
ORIGIN      
        1 tggatctccc cgggcttcat ccgggcgatg accgtcgccc cgtcagcctt cacttcgccc
       61 aggctcacgt cgagggaagg gaactcctcc ttgagggtgg cctgcgcacg ggtggcgaag
      121 tcgcccgggg gaaaccggag agagaacccg ccgtcagcga ccttctggaa ggcgaggacc
      181 ggcagcgcct tctcacggca ggcggcgcgc gcgtcgtccg cgtagcgcat ggaggtgttc
      241 tcgatcgcct tgtcgatttc gacggccagg cgaaggaaca cgcccccctg gaggtcgagt
      301 ccgaggttga tcttcgggac ccggtctttc cagcccgcgg ggacctggtc ggtcagcgag
      361 gggaccagaa tgatgatgga gaccgccgtg aggacggcga tcagcgtgat tcttccggtg
      421 atgctcttcc acataatccg ctcgtgctcc tttccgtttg aaactcagac tccattattt
      481 atgaatcagc acctaggccg gtttctgcgc ctcggggacg gccgcgtcct ggctcgagcc
      541 ggagacggcg ctgcgggtga ccttcacgcg gaccttgtcg gcgatctcga gggtgacggt
      601 cgtctcggtc aatcccttca cctcgccgtg aagccccccc gcggccacca cccggtcgcc
      661 aaccttgagg ttcttgatga agtcgcgttg cttgctcgcc tgtttctgct gcggacggat
      721 cagcaggaag tagaagatga cgaacatcag caggatcggg agaagcccca tgaggccgcc
      781 gccccctccg gccccgccgc ccggggatcc gcccatcgcg tacgccacgc cggtcatgaa
      841 catagggttt cgcctccttg gccgttactc gttttccgaa acgactgatt ctttcacaag
      901 ttccccgaaa tttccacggc aaatcgcctc ccgcgccttc cgcatcatcc gcgcgtagaa
      961 atgaaggttg tgcatggtca tcgccatcga tcccagcatc tcccgctgga gatagaggtg
     1021 gcgcaggtac gcgcgggaga aggcgcggca cgtggggcag tcgcaccgct cgtccggcgg
     1081 aagggagtcg tccgcgtacc gggcctgctt gatcgaaacc gaacccgcgg aggtgaacat
     1141 catcccgttc cgcgcgttgc gcgtcggtag aacgcagtcg aacaggtcca ccccccgcgc
     1201 gatcgcaaag aggatgtcct ccggtgttcc cacccccatc aggtagcgcg gcatggaagc
     1261 gggaagcatc ggcgccgtcc cggccacggt ctcgcgttgg aactcctttc cctcaccgac
     1321 gcttaccccc ccgatggcga atccctgaaa cgggagtgcg cagatctcct cgacgctgcg
     1381 ccgccgcagg tccgggaaca tacccccctg gacgatcccg aacatgccgc cgccctcgga
     1441 agtccgcgcc gcgagggagc gcgtcgccca caacgtcgtc cgccgcaccg cctcctggac
     1501 ctcttccctg cccgccgggt attccacgca ctcgtccagc gccatcctca cgtccgaccc
     1561 gagcgcctcc tgtaccgcca ccgcgagctc cggggtgagt gtgtgcgccg acccgtcgag
     1621 gtgggatcga aacgtcaccg cctcgtcgga caccttccgc agggcgctga gggagaagac
     1681 ctggaaccct ccgctgtcgg tgaggatcag gcggtcccac cccatgaacc ggtggagccc
     1741 cccctggcac cggattcgct cgtggccggg gcgcaggtac agatgatacg tgttgccgag
     1801 gatgcagccg aatcccatct cccgcagctg gtccggccag acccccttga ccgtcgccgc
     1861 cgtccccacg ggcatgaacg ccggggtgcg caggatgccc cgatcggtcg agatcgtgcc
     1921 cgccctcgcc gcggtgccgc cgtcccgcgc ctcgaccgcg aaccggagcg tcaaagggcc
     1981 ctcctcaaga gatgaacatc gcgtcgccgt agctgtagaa gcggtattcc cgcgccaccg
     2041 cttcccggta cgccgcccgc accgcgtcga cgccggcgaa cgccatcacc agggcaagga
     2101 gacttgatcg cggaaggtgg aagttcgtca cgagggcgtc gaccgcccgg aaccggtagc
     2161 cggggaagat gaacttccgc gtcgtccccc ccgacggctc caacgtgccg tcctccgccg
     2221 cgcacgtctc cagcgttcgc acgctggtgg tgcccaccgc gaccacgcgc ccgccgcgac
     2281 cgcgcgccgc gttgatttcc gccgccgtct ccggcggcaa gcggtaccgt tccgggtgaa
     2341 tcccgtacgc ctcgacctcc tccgtgcgga tcggggagaa ggtgccgtac ccgaccgaca
     2401 gggtcaaccg cgcgacgccg actcccgccg ttgcgagctc ccccagcaga tcctcgtcga
     2461 aatgaagtcc cgcggtcggc gccgccaccg agccggggtt cctcgcgtac accgtctgat
     2521 accggaccgc atcctccttc gacgcgggat cgtcggggcg ccgccggatg tacggcggca
     2581 gcggcacctc ccccgcccgc tccagcgcct tcgcgacgga gacgctcctc cctgacagga
     2641 gcacctccca ccgtccctcg tccagcctgc gcgcgaggcg gacccgcagg gtccccccga
     2701 cctcgaactc ctctccctcc ttcagccgct tcgaaggacg ggcgagcgcc tcccaacgtt
     2761 cctcccccgc ggctccggcc tcctgcaggg gcgacaggag gaagacctcg acggcccccc
     2821 ccgtcctgcg cgacgcgcga atccgcccgt ggagaacctt cgtatcgttg acgaccacca
     2881 ggtccccgcg gcgaagaagg aacggcaatt ccggaaacac gcggtggccg atctcccccg
     2941 tcgcgcggga gagggtcatc aggcgggcgt cgcgccggcg gggaggcgga tgctgcgcga
     3001 cgagtcgctc cggcaggtcg tactccagca gatcggtcct cagggcgtct ctccgatgat
     3061 ttcggtcccg gggtagtagc gggcgaggat ctcgcgaaat cccgcgccgc gccaggccat
     3121 cccgtttgcc ccgaactggc ataaccccac cccatgaccc cacccttccc ccgtaaaccg
     3181 ccattccccg gcgacgggga cgatctccat cttcaggctg cggaccttcg cgtacccggc
     3241 cgccttccgg aactccgcgg cctgcagctc ccgggacacg ccgccggagg agatccggac
     3301 gcggctcg
//
LOCUS       DASVJL010000483        10268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1214539, whole genome shotgun sequence.
ACCESSION   DASVJL010000483 DASVJL010000000
VERSION     DASVJL010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10268
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214539"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /gene="trpD"
                     /locus_tag="VF357_09960"
     CDS             complement(<1..1128)
                     /gene="trpD"
                     /locus_tag="VF357_09960"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX7524517.1"
                     /translation="MIAVIDNYDSFTWNLVQYIRELGAEVAVFRNDAITVEELALRNP
                     VGLVISPGPGGPNEAGISLDAIRAFEDRIPILGVCLGHQCVGQAFGGRIVHAQALMHG
                     KTSRVRHNGKGIFSMVENPMTAMRYHSLAVERATLPPELEVCAETEDGEVMGVRHLEK
                     PVFGVQFHPESILTQSGMRLLENFLSLIDPSQPVLREFGNIREAIAAVSGRKNLSEDG
                     MRDAMRMIMGGEATPAQVASFLSCLSMKGETITEIAAAAEVMRQKAIRIVPPAGKEVL
                     DTCGTGGDRAGTFNISTTVAFVAAGAGVAVAKHGNRSVTSRSGSADVLEALGMDLNAD
                     PVLVQRALDEVGITFMFAPRFHAAMKYAIGPRREIAIRTIFN"
     gene            complement(1125..2624)
                     /gene="trpE"
                     /locus_tag="VF357_09965"
     CDS             complement(1125..2624)
                     /gene="trpE"
                     /locus_tag="VF357_09965"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX7524518.1"
                     /translation="MIVPDKAQFLRQAEEGTLIPVFREFHADLETPVSAYLKISARFP
                     TDHFLLESVEGGEKWARYSFIGFDPHLRFRADSNGITVRQGAVSRNVPATGDPLDVLA
                     GILKEIRYCPAPGLPRLSGGAVGYIGYDYVRYLEKVGGDKPLTDAPDAMFLFPSRLVI
                     FDNVRHTILIVAHGSVVAGENPGAAYARALEAIDEAREILRVPLEGSEVPDGDDGGET
                     PAFEMSRGTFLDAVGKAKEHIRNGDIIQAVLSNRATVRTRRTPAEVYRVLRALNPSPY
                     MYLLRMGDLSVVGSSPEILVRLEGDEIQLRPIAGTRPRGATPSEDRRLEVELLSDPKE
                     LAEHVMLVDLGRNDVGRVAAWGSVKATELMGIERYSHVMHIVSNVVGKLREGLTAFDV
                     LRAAFPAGTVSGAPKVRAMQIISELEPFRRGIYAGAVGYFDLQGSMDFCIAIRTIVMS
                     GGEAMIQAGAGIVADSDPAREWDEILSKAKILFRAVGVSPEALEALEAP"
     gene            complement(2634..3302)
                     /locus_tag="VF357_09970"
     CDS             complement(2634..3302)
                     /locus_tag="VF357_09970"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX7524519.1"
                     /translation="MRSLRHKLGFRRGDPERRSFAFFVVGTLVVIAVAFFLGFQLGRY
                     IEKSAGKETEGKIAPRGAAVENGARIVTSAEIRKDMSAFSEEALKIPAVAPPAAIPPT
                     AREDLQKTESEATFPESLSRKDPSPEPMGKIKKKPQVSAPSVEAKLMLQAGAMKTRET
                     AEALRRRLERSGYKAKVIHATTQKFGEVYRVRVGPFGSRDEAMKAMKAIRAEMKIDVI
                     LLKG"
     gene            complement(3299..4963)
                     /gene="argS"
                     /locus_tag="VF357_09975"
     CDS             complement(3299..4963)
                     /gene="argS"
                     /locus_tag="VF357_09975"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX7524520.1"
                     /translation="MDARRIVESLVFSAVRKKVAEWGEDAEVSVSLEIPRQESHGDFS
                     TNAAMQLAGRLGRKPRTVAEEIVSALRDEDQRGRFASLSIAGPGFINIVLSEEFWREV
                     LTVALEKGPRFGASKVGEGKTVHLEFVSANPTGPLHVGHGRGAAVGDALARILEFTGH
                     KVVREYYINDVGNQMDNLGRSLFARYRVLCGRPSELPEDGYRGEYMIELARDFREEVG
                     DRYADSPEEVALPVFRKEAGDRILRGIRDDLASFRVSYDRWFPEKELHDRGLVAAALA
                     DLRERGQLYEADGATYFRSQALGDEKDRVLVRADGRTTYFAADVAYHRHKLREGFSRM
                     IDIWGADHHGYVPRLRAALRGLGEDENRLEVLLVQFVTLVREGKPVQMSTRSGEFTTL
                     REVLDEVGVDAARFFYLLRSFHTHLDFDLTLAKTQSRNNPVYYIQYVHARICSVFREA
                     EAQGRTLAGHPPLSILTFPEEVRLMKAVARFPDVVAECAKTLEPHRIPFYLIEVSDLF
                     HAFYHQHRFLGESPERTQARLALALGVKTVVASGLSMIGVTAPERM"
     gene            complement(5046..6572)
                     /locus_tag="VF357_09980"
     CDS             complement(5046..6572)
                     /locus_tag="VF357_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX7524521.1"
                     /translation="MAESSLLRREEELKDGIRQALDRLLGLTGASHVAYIGRSVSPGS
                     VLHEGFLLSRGSPVSREISLSDTYVPLREATVFRKPFLEVGSGARRYAPWKSGPGSAP
                     AGVAAVPVFRDEMVEAVLLAVRDEEGPWRDPVIPAMELAAHFVGRDIERMRELHQGDR
                     YFLRGEWFHRMVRKMAEVGTEGDPEPGGGMRSRREMVYAETSEQVRRQVDADRVLLVE
                     SSEDPGNGRVVWAISPDGGGPGPEAYEPLGDSYVGWVIRTGTQRIFPGTAGPPRSQGV
                     LPGSWEREGERSFLVLPVEGEVGFRGALVCAHSQEKRFRKRHAEIARDITRVMQLGLS
                     HVERLESLTKKATTDGLTGLSNRKAFLERLAVDLARLDGRHPCGVVMLDIDHFKGIND
                     TYGHPFGDEVLRGVASVLGKGVRKGDTAGRYGGEEFVLYLHMADTERAREGAERFRRI
                     IRQIRFAHAGKEIAVTASFGVACATHHGKGVEELLKHADEALYLSKQRGRDRVTVYPG
                     "
     gene            complement(6533..6919)
                     /locus_tag="VF357_09985"
     CDS             complement(6533..6919)
                     /locus_tag="VF357_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524522.1"
                     /translation="MKRYRYAAGLSAPVYLLALLLLRERISGGIPFLLSTAIGPVAAA
                     YLFAAYLLDWKRVDRVPDLLPLLVWGGVAAMEIHAFVPAVARTGVVPAALFFGFALKF
                     PPMLSIPAILCADAWLNPVCSGGKRN"
     gene            complement(6940..7320)
                     /locus_tag="VF357_09990"
     CDS             complement(6940..7320)
                     /locus_tag="VF357_09990"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX7524523.1"
                     /translation="MGRNTRLTNMTIPLRDPNRKKRRLPLYIAAALVIAAVFISLFRD
                     MGVVDSWRLRKTERQLRGEVEKLRQENALLKRTVEDLRGNPAVIEQEARRLGLIKEGE
                     NVIVVPRRQDSGPQPAPKPSTNRP"
     gene            complement(7366..8637)
                     /locus_tag="VF357_09995"
     CDS             complement(7366..8637)
                     /locus_tag="VF357_09995"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX7524524.1"
                     /translation="MRSTSNRRSAVALLLCGTLLWQSATTVAGAEAGASTDPALLLKY
                     AAPDLPDRSARETLDPIPAIVAKELRIRWLPVPMDPSGKTPSTGEIPVPDDAALRRIA
                     GKVSRASEQMERVESAAAERLLDEAEKECRSYRFTEATRPFLAEIFLRRGILRLWEGK
                     ASDAEALFSRVRALRPGFTPDPALFPPQVRSIWKAIGHRPVPEAELLVESLPSGAEIF
                     VDEERRGVTPARVRIKKFAPVRIRVSHPGYRDAETKGQWLPGDTEILRFSLPGDRVAR
                     LGELLAGAALGKGGGAGPLVEELSAAAGTPRVAILMLEKGAAGVGLRARLYAGRPAGV
                     DPALLGETSFPEGKAGAEISGKWVADTLAADGWPRAERPERPWYKSRWFWGIFLAIGA
                     AAAFGVGGGGGGGGGGGGTGGSSGGTVAVNF"
     gene            complement(8612..9259)
                     /locus_tag="VF357_10000"
     CDS             complement(8612..9259)
                     /locus_tag="VF357_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX7524525.1"
                     /translation="MTEHGQDEILELLWTLREERKANREEVLRTSPEPGPDHLLEELV
                     EGGMVDASGEEILLTKSGEDRARGIIRRHRLAEVLLQNLFDLDNTQMENSACQFEHIL
                     SEPVVESVCTFLGHPPACPHGRPIPRGECCDRIRTEIRPLVMRLTEASLGATVRIVFI
                     TPRSKKRLEKLSALGIVPGSRVRLLQRNPSFVLEIGQTTVAVDRDITDEIYVKPT"
     gene            complement(9462..>10268)
                     /locus_tag="VF357_10005"
     CDS             complement(9462..>10268)
                     /locus_tag="VF357_10005"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX7524526.1"
                     /translation="RLAVMVDRLFKKIGCNGKAVLPMILGLGCDTMATLTTRILETRK
                     ERMIITLLLALGVPCAAQLGVILGMLSDVGPVATITWLGLMVGVILAVGFLASKVIPG
                     ESSDFILEIPPIRWPQVGNLAIKTMARIEWYLREAVPLFLVGTLVLFVADRLGWLLRI
                     QAAAEPVIVHMLDLPPKATEAFLIGFLRRDYGAAGLYSMAKGGMLTHLQVVVSLVTMT
                     LFMPCLANFLVIVKEHGAKVAIWMSLFIFPFAVLVGAAVNFFSRWAGITF"
BASE COUNT         1817 a         3521 c         3177 g         1753 t
ORIGIN      
        1 gttgaagatc gtccggatgg cgatctcccg tcgcgggccg atggcgtatt tcatcgcggc
       61 gtggaacctg ggagcgaaca tgaaggtgat cccgacctcg tcgagggcgc gctgcaccaa
      121 cacggggtcc gcgttcagat ccatgccgag cgcttccagc acgtccgcgc tcccggaccg
      181 gctggtcacg gaccggttcc cgtgcttggc cacggcgacg ccggcccccg cggcgacgaa
      241 cgccacggtg gtggaaatat tgaacgtacc ggcgcgatcc cctcccgttc cgcacgtgtc
      301 gaggacctct ttccccgccg gcggaacgat gcggatcgcc ttctgccgca tcacctcggc
      361 ggccgcggcg atctccgtga tcgtctctcc cttcatggag aggcaggaga ggaacgaggc
      421 gacctgggcg ggggtagcct ccccacccat gatcatccgc atcgcgtccc gcatcccgtc
      481 ttccgacaga ttcttgcgtc ccgacacggc ggcgatcgcc tcccggatgt tcccgaactc
      541 gcgcagaacc ggctgggacg ggtcgatcag ggaaaggaaa ttctcgagga gccgcattcc
      601 cgactgggtc aggatcgact ccgggtggaa ctgcacacca aagaccggtt tttcgaggtg
      661 gcgtaccccc atcacctcgc cgtcctcggt ctcggcgcaa acctccagtt ccggaggaag
      721 ggtcgcgcgt tccacggcga gggaatggta ccgcatggcg gtcatcgggt tctcgaccat
      781 cgagaagatc cccttgccgt tgtgccggac gcgggaggtc tttccgtgca tgagcgcctg
      841 ggcgtgaacg attcgtcctc cgaacgcctg cccgacgcac tggtgcccca ggcagacccc
      901 gaggattggg atccggtcct cgaacgcccg gatcgcgtcg agagagatcc cggcctcgtt
      961 cggtccgccc ggccccggcg agatcaccaa cccgacgggg ttccggagcg ccagttcctc
     1021 aaccgtgatc gcgtcgtttc ggaacaccgc cacctcggct ccgagttcgc ggatatattg
     1081 cacgaggttc catgtgaacg aatcgtagtt gtcgatgacg gcgatcatgg cgcctccagc
     1141 gcctccagcg cttccggcga cacgccgacc gcgcggaaga ggatcttcgc cttgctcagg
     1201 atctcgtccc actcccgcgc cgggtccgaa tccgcgacga tccccgcccc ggcctggatc
     1261 atcgcctccc cccccgacat cacgatggtc cggatggcga tgcagaagtc catgctgccc
     1321 tgcaggtcga aatatcccac cgcccccgcg taaatccccc ggcggaacgg ttcgagttcg
     1381 gagatgatct gcatcgcccg gactttcggg gcgcccgaaa ccgtccccgc gggaaacgcc
     1441 gcgcggagta cgtcgaacgc ggtgagcccc tcgcgaagct tcccgaccac gttcgaaacg
     1501 atgtgcatga cgtgcgagta ccgctcaatc cccatcagtt cggtcgcttt cacggatccc
     1561 cacgcggcga cccggcccac gtcgttgcga ccaagatcca cgagcataac gtgctccgcc
     1621 agctctttcg gatccgacag cagctcgacc tccagccggc ggtcctccga gggggtggcg
     1681 ccccgggggc gcgtgcccgc aatcgggcgg agctggatct cgtccccctc gagccgcacg
     1741 aggatctccg gggaggaacc gacgaccgaa agatccccca ttctcagcag gtacatgtag
     1801 ggggagggat tgagggcgcg aaggacgcgg tacacttcgg cgggagtccg tcgcgtgcgt
     1861 acggtcgccc ggttggagag caccgcctgg atgatgtcgc cgttccggat gtgctccttg
     1921 gctttgccca ccgcgtcgag gaacgtcccg cgcgacatct cgaacgcggg ggtctcccca
     1981 ccatcgtccc cgtccgggac ttcggatcct tccagcggca ctcggaggat ttcccgagct
     2041 tcgtcgatgg cctctagcgc ccgcgcgtag gccgcgcccg ggttctcccc cgcgacaact
     2101 gaaccgtgag cgacgatgag gatcgtgtgc cggacgttgt cgaagatcac gagccgggac
     2161 ggaaagagga acatcgcgtc gggggcgtcg gtcaacggct tgtctccccc gaccttttcg
     2221 aggtatcgga cgtagtcgta tccgatgtaa ccgacggccc cccccgaaag tctcgggagt
     2281 cccggcgccg gacagtaccg gatctccttc aggatccccg cgagcacgtc gagcgggtcc
     2341 cccgtggccg gaacgttccg cgacaccgct ccctgtcgga ccgtgatccc gttcgaatcg
     2401 gcgcgaaacc ggagatgagg gtcgaaaccg atgaaggagt accgggccca cttctcccct
     2461 ccctcgacgc tctccagcag gaagtggtcg gtcggaaacc gggccgatat cttcaggtag
     2521 gcggagaccg gggtctcgag gtcggcgtgg aactcccgga agacgggaat cagcgttcct
     2581 tcctccgcct gacggagaaa ttgtgccttg tcggggacaa tcacggcgtc acctcatccc
     2641 ttcagcagga taacgtcgat cttcatctcg gcccgtatcg ctttcatcgc cttcatggcc
     2701 tcgtcgcggg atccgaacgg ccccacgcgg acgcggtaca cctccccgaa cttctgggtc
     2761 gtcgcgtgga tcactttcgc tttgtatccg ctccgttcga gtcgcctccg caatgcctcg
     2821 gcggtttcgc gcgttttcat cgcacccgcc tgcagcatga gcttcgcttc cacggatggg
     2881 gcggaaacct gcggcttctt cttaatcttc cccatcggtt ccggagaggg atccttccgg
     2941 gagagtgatt cggggaaggt ggcctcggac tccgtcttct gcagatcttc ccttgcggtc
     3001 ggggggatcg ccgcgggagg ggcgaccgcc gggatcttca acgcttcctc cgaaaatgcc
     3061 gacatgtcct tccggatctc cgcggaggta acgatccgcg cgccgttctc caccgcggca
     3121 ccccgaggag cgatttttcc ctccgtctcc ttccccgcgc tcttctcgat gtatctcccc
     3181 aactggaagc cgagaaaaaa agcgaccgcg atcacgacga gggtgccgac gacgaagaac
     3241 gcgaaggagc gccgctcggg atccccgcgt cgaaacccaa gtttgtggcg gagcgatctc
     3301 acatccgttc cggagccgtc acgccgatca tcgaaagccc ggacgcgacc acggtcttca
     3361 cgccaagggc gagtgcgagc cgggcctgcg tccgttcggg agattccccc aggaaacggt
     3421 gctgatggta gaaggcgtga aagagatccg aaacctcgat aagatagaag ggaatcctgt
     3481 ggggctccag cgtcttcgcg cactccgcga cgacgtcggg aaaccgggcg accgccttca
     3541 tcagccgaac ctcctcgggg aaggtgagga tggaaagggg cggatgtccg gcgagcgtcc
     3601 tcccctgggc ttccgcctcg cggaaaacgg agcagatccg tgcgtgcacg tactggatgt
     3661 aatatacggg gttgttccgc gactgggtct tcgccagggt gaggtcgaag tccaggtggg
     3721 tgtggaagct gcgcaacagg tagaaaaaac gggcggcgtc gacccccacc tcgtcgagga
     3781 cctcgcgcag cgtcgtgaat tctcccgacc gggtcgacat ctggaccggc ttcccctccc
     3841 ggaccagtgt cacgaactgg acgagaagca cctccaggcg gttttcgtct tccccgagcc
     3901 cccgaagcgc cgcgcgcagc ctcggcacat acccgtgatg atcggccccc cagatgtcga
     3961 tcatccggga gaatccttcc ctcagcttgt gccgatgata cgcgacgtcc gccgcgaaat
     4021 aggtggttcg cccgtccgcg cgaaccagga cgcggtcctt ttcgtccccc agcgcctggc
     4081 tgcgaaagta ggtcgcccca tcggcttcgt agagttgtcc gcgttcccgg agatccgcaa
     4141 gggcggcggc gaccagcccc cggtcgtgca gttccttttc cgggaaccac cggtcgtacg
     4201 acacccggaa cgacgcgagg tcatcgcgaa tcccgcgaag gatgcgatct cccgcctcct
     4261 tcctgaagac gggaagcgcc acctcctccg gcgagtcggc gtaccggtcc cccacctcct
     4321 cgcggaagtc ccgtgccagc tcgatcatgt actccccgcg atacccgtcc tcggggagct
     4381 cggagggacg tccgcacaga acacggtacc tcgcgaagag cgagcgcccg aggttgtcca
     4441 tctggttgcc tacgtcgttg atgtaatact cgcggacgac cttgtggccg gtgaactcca
     4501 gaatccgggc gagggcatcc ccgacggcgg cgccgcggcc gtgaccgacg tgaaggggtc
     4561 cggtcgggtt ggccgacacg aattcaaggt ggacggtctt tccctccccg accttcgagg
     4621 cgccgaaccg cggccccttc tccagggcga ccgtcaggac ttccctccag aactcctcgg
     4681 acaggacgat gttgatgaac cccgggccgg cgatcgaaag ggacgcgaag cggccccgct
     4741 gatcctcatc gcggagcgcc gacacgattt cctccgccac cgtcctgggt tttctcccga
     4801 gacgcccggc gagttgcatc gccgcgttgg tggagaaatc cccgtgcgac tcctgccggg
     4861 ggatttcgag agagacggaa acctccgcgt cctctcccca ttccgcaacc tttttccgaa
     4921 ccgcggagaa gaccagggat tcgacgatcc gacgtgcgtc caaacagcgc ctcgaaggga
     4981 aaatgattgt aaaggctgca gtttatcccc ctcgacgggg ggtgtcaacg cgacgcgacg
     5041 atgcttcagc ccgggtagac cgtcacccgg tcgcggccgc gctgcttgga gaggtagagc
     5101 gcttcgtcgg catgcttcag gagctcctcg acccccttgc cgtgatgggt agcgcacgcg
     5161 acgccgaaag aagcggtgac ggcgatctcc tttcccgcat gggcgaatcg gatctgacgg
     5221 attatacggc gaaacctctc ggcgccctcc cgggcccgct cggtgtccgc catgtggagg
     5281 tagagcacga attcctcccc tccgtaccgt cccgccgtgt cgcccttgcg gactcctttg
     5341 ccgaggaccg acgccacgcc ccgcagcacc tcgtccccga acgggtgccc gtaggtgtcg
     5401 ttgatcccct tgaagtggtc gatgtccagc atcacgacgc cgcacggatg ccgaccgtcg
     5461 agccgtgcaa gatcaaccgc cagcctctcg agaaacgcct ttcggttcga cagtccggtc
     5521 aacccgtccg tcgtcgcctt tttcgttagc gactcgagcc gttcgacgtg ggaaagcccg
     5581 agctgcatga cccttgtgat gtcccgcgcg atctccgcgt gccgtttccg gaaccgtttc
     5641 tcctgcgagt gggcgcagac caaggcgccc cgaaacccga cctcgccctc caccggcagg
     5701 accaggaacg accgctctcc ctccctctcc cacgaacccg gaaggacacc ctggcttcga
     5761 gggggaccgg cggtgcccgg aaagatgcgc tgcgtcccgg tgcggatcac ccacccgacg
     5821 taactgtcac cgagcggttc gtacgcctcc ggccccggcc cgccaccgtc cggcgatatc
     5881 gcccagacga cgcgaccgtt ccccgggtcc tcgctggatt cgaccagaag gacccggtcc
     5941 gcgtcgacct gacggcggac ctgttccgaa gtctccgcgt agaccatctc ccgcctcgaa
     6001 cgcatgccgc cgcccggctc cggatcccct tccgtcccca cctccgccat cttccggacc
     6061 atccggtgga accactcccc gcgaagaaag taccgatccc cctggtgtag ttccctcatc
     6121 cgctcgatat cgcgtcccac gaaatgagcc gccagttcca tcgccgggat cacgggatcc
     6181 ctccacgggc cctcctcgtc gcggaccgcg aggaggacgg cttccaccat ctcatccctg
     6241 aataccggga cagcagccac tccggcggga gcgctcccag gaccgctttt ccacggagca
     6301 taccgcctcg cacccgaccc aacctcgaga aacggtttcc tgaaaacggt tgcctcccga
     6361 aggggtacat atgtgtcgga aagggagatc tccctggaga ccggcgagcc gcggctcagc
     6421 aggaacccct cgtgaagaac ggatccgggg gatacggacc ggccgatgta agcgacgtgg
     6481 gaagcgccgg tcagccccaa caggcgatcc agcgcctgcc ggatcccatc cttcagttcc
     6541 tcttccctcc ggagcagact ggattcagcc atgcgtcggc gcaaaggatc gccggaatgg
     6601 agagcatcgg gggaaacttc agggcgaagc cgaaaaagag ggccgcaggg acgacgccgg
     6661 tcctggcaac cgccggaacg aaggcgtgga tctccatggc cgcgacgcct ccccagacga
     6721 gaaggggaag caggtcgggg acacggtcga cccgtttcca atcgagaaga taagcggcga
     6781 agagatacgc ggccgcgacc ggtccgatcg cggtggaaag gaggaatgga atccctcctg
     6841 agattcgctc ccggaggagg aggagggcaa ggaggtatac cggggcggac aaccccgcgg
     6901 cgtacctgta ccgtttcaat gcgtcactgc gatatggcac taaggacggt tcgtgcttgg
     6961 cttgggggcg ggctgcggac cggagtcttg ccgccggggg acgacgatga cgttctcccc
     7021 ctctttgatg aggccgagcc ggcgggcctc ctgttcgatg accgccgggt tgccgcgaag
     7081 atcctcgacc gtgcgcttga gcagcgcgtt ctcctgtcga agcttttcga cctcgccgcg
     7141 aagctggcgt tccgtcttgc ggagtctcca actgtcgacg acccccatgt cgcggaatag
     7201 cgagatgaat accgccgcga tcaccagggc cgccgcgatg tacagcggca gacggcgctt
     7261 tttccggttc ggatccctca aggggatcgt catgttggtc agtctcgtgt ttcgtcccat
     7321 ggtccgggga acatcatatc cgaaaacccc ggtctcgttc gatggtcaaa aattcacggc
     7381 gacggtaccc cccgaggaac cgccggtccc gccgcctccg ccgcctcccc cgccgccccc
     7441 gcccaccccg aacgcggctg ccgctccgat ggccaggaaa atgccccaga accagcggct
     7501 tttgtaccag ggtctctccg gacgttccgc tctgggccac ccgtccgcgg caagggtgtc
     7561 ggcgacccac tttcccgaga tctccgcccc cgccttcccc tccgggaacg acgtttcccc
     7621 gagaagagcc ggatccacgc ccgccggtct tccggcatag agccgggcgc gcagcccaac
     7681 gcctgccgcc cccttttcga gcatcaggat cgcgacgcgc ggtgtccccg ccgccgcgga
     7741 gagttcctcg accagggggc ccgcgcctcc gccttttccc aacgcggcgc cggcaagaag
     7801 ttcgccgaga cgagccaccc gatcgccggg aagggagaag cgcaaaatct ccgtgtcccc
     7861 cggaagccat tgacccttcg tctcggcatc tctgtatccc gggtgggaga cgcgtatccg
     7921 taccggcgcg aactttttta ttcgaacgcg agccggcgtg actcctcgac gctcttcgtc
     7981 gacgaagatc tccgcgccgg aggggagcga ctctaccagg agttccgctt caggtacggg
     8041 gcggtgcccg atcgctttcc atatggaccg tacctggggg ggaaagagtg cggggtccgg
     8101 ggtgaagccc ggacgcaacg cccgcacccg ggaaaagagc gcctcggcgt cggatgcctt
     8161 cccctcccag aggcggagaa tcccccgccg gagaaaaatt tccgcgagga acgggcgcgt
     8221 cgcctccgtg aatcggtacg accggcactc cttctcggct tcatcgagaa gccgttcggc
     8281 ggcggcgctc tcgaccctct ccatctgttc ggaggcgcgg gacaccttcc cggcgatccg
     8341 tcgcagggcc gcgtcgtcgg gaaccgggat ctcccccgtg gacggagtct tccccgaagg
     8401 atccatcggg acggggagcc accggatccg gagctccttc gcgacgatcg ctggaatcgg
     8461 gtcgagggtc tcgcgcgcag aacgatcggg cagatccggc gcggcgtatt tcagcagaag
     8521 cgcggggtcc gtcgacgccc cggcctccgc gccggcgacc gtcgtcgccg actgccagag
     8581 cagcgttccg cacaggagaa gcgcgacggc gctacgtcgg tttgacgtag atctcatccg
     8641 tgatgtcccg gtcgacggcg accgtcgtct gcccgatttc gagtacgaag gaggggttcc
     8701 gctgaagaag gcgaaccctg cttccaggaa cgatcccgag cgcggaaagc ttctccagcc
     8761 gcttcttcga gcgcggggtg atgaagacga tccgcacggt ggccccgagg gacgcctcgg
     8821 taaggcgcat gacgagggga cggatctccg tccggatccg gtcgcagcat tctcctctcg
     8881 ggatcgggcg gccgtgcggg caggcgggag ggtgacccag aaaggtgcac acgctctcga
     8941 ccacgggctc ggagaggata tgctcgaact ggcaggcgct gttttccatc tgcgtgttgt
     9001 ccagatcgaa caggttttgc agaagcacct ccgcgaggcg atgacgccgg atgatcccgc
     9061 gggctcggtc ctctccgctc ttcgtgagga ggatctcttc tcccgaggcg tcgaccatgc
     9121 cgccctcgac cagctcctcg agaaggtggt cggggcccgg ctccggggaa gtgcgcagca
     9181 cctcctcgcg gttcgctttc cgctcttccc gaagcgtcca gagaagctcg aggatctcgt
     9241 cctggccgtg ctcggtcatt tccggcttcc tttcaggaaa aggctcttca cgaaattcac
     9301 cactttcgac tcgggcatgg ggaaagagta tctgcatcgc gggcaacaga caagcgtgca
     9361 tcctttactc atggggcacc cggagcggca ggcgcggtcc gtcggatcga tctcccattg
     9421 gcacagcggg catcggatct tcacattgtc cccccgccgg atcaaaacgt gatccccgcc
     9481 cagcgcgaga agaagttgac cgccgcgccg accagcacgg cgaaggggaa gatgaagagg
     9541 gacatccaga tcgccacctt cgctccgtgc tccttgacga tcacgaggaa gttcgcgagg
     9601 cacggcatga aaagcgtcat ggtgaccagg ctcaccacca cctggaggtg ggtgagcatc
     9661 ccccctttcg ccatgctgta cagtcccgcc gccccgtagt cccggcgcag aaagccgatc
     9721 aggaatgcct ccgtcgcctt cggtgggagg tcgagcatat ggacgatcac cggttccgcc
     9781 gcggcctgaa tccgcagcag ccaacccagc cggtccgcga cgaaaagaac gagggtgccg
     9841 acgaggaaaa ggggaaccgc ctcccgcagg taccattcga tccgcgccat cgtctttatg
     9901 gcgagattcc ccacctgggg ccagcggatg ggcggaatct cgaggatgaa gtccgacgac
     9961 tccccgggga tcacctttga cgcgaggaag ccgacagcga ggatgacccc caccatgagg
    10021 ccgagccagg tgatcgtcgc gacaggaccg acgtccgaca acattccgag gatcaccccc
    10081 agttgcgccg cgcacggtac ccccaacgcc agcagcaacg tgatgatcat ccgctccttg
    10141 cgcgtctcca ggattcgcgt ggtcaaggtc gccatcgtgt cgcacccgag gccgaggatc
    10201 atcggaagca ccgccttgcc gttgcagccg atcttcttga aaaggcgatc caccatcacc
    10261 gcgagccg
//
LOCUS       DASVJL010000484         3428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_181375, whole genome shotgun sequence.
ACCESSION   DASVJL010000484 DASVJL010000000
VERSION     DASVJL010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181375"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VF357_10010"
     CDS             complement(<1..1087)
                     /locus_tag="VF357_10010"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7524527.1"
                     /translation="MNRNTESAQPPELALAGDRPPATLAARVENGVSIGLLVGMAILP
                     ILEIVGRRVWRTGVPGSSALVQHATLWIGLLGGAIAARDDRLLSIGHLADRFREPYRR
                     VLSSFVAAVSAAVSLLLAGSGLQLVRAEWADRHSVVSFLPLWVAEGVIPAGFALIAWR
                     LVRRANGGVWTRAAAAAGAVVGVAFVSSGLILDSVPFAASIIILSAAVILGAPLFAAL
                     GGFAVLFFRHADVPLASISAEIYRIVTSPTLPTIPLFGFAGYVLTVGNVSRRLVRFFR
                     AIVGWMPGGIAVVAVLVCTFFTTFTGASGVTIVALGGILLPALLVEGYPERFSLGLLT
                     SSGSLGLLFPPCLPVILYAVVAGIAVDK"
     gene            complement(1276..2319)
                     /gene="dctP"
                     /locus_tag="VF357_10015"
     CDS             complement(1276..2319)
                     /gene="dctP"
                     /locus_tag="VF357_10015"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX7524528.1"
                     /translation="MNCSSIRRSAAVLLVAIAALSAPVGAMPAPTVVKMATLAPEGSS
                     WFRVLQEMGEDWRKASNGEVTLRIYPGGVAGDEDAMIRKMRIGQIQAAAITGIGLAYL
                     ELSFYALHIPMMYESDEEFDSVRDRYAPVLERKMEEKGFVVLNWGDAGWVHFFSKTPV
                     ITPAEAKGLKLFQWEGDTNLVQLYKETGFHPVPLSTNDLLPGLQTGMVNGFTSTPLVA
                     LAFQWFGLAPHMADLRYAPLTGATVIEKRAWEKIPPGLRPKLLEASRRAGLRLRAEIR
                     RLNQEALEVMVKNGLKIHMVPPEVQAQWRKMVEDNYPRIRGNIMPAEAFDTVKKFRDE
                     YRAAHRGAKGAGR"
     gene            complement(2300..3211)
                     /locus_tag="VF357_10020"
     CDS             complement(2300..3211)
                     /locus_tag="VF357_10020"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HEX7524529.1"
                     /translation="MPLPPRRFSPLLLAACFLLACSPRSYVVSRMADAASSGGEVFSR
                     DDDPELVRDAVPFALKAMESLLASSPDHKGLLTALSKGFTQYAVAFVRQDAEEARDPV
                     TRRAGMERTRRLLLRAREYGVRGLSVGRQGFPTGLVEDPSAAAARFDAEDVPLLYWTG
                     ASWSLAVSTSSDDPSLLADLPRIEALMRRALALDERYDAGAIHEFFVAFEGGRPEAMG
                     GSVERARHHMERAMALSAGKKISPLVTFAETVSVRTQDRKGFLDLLDRALAFDARSAA
                     PEYRMGNLVTQRRARWLKGRVDELFLD"
     gene            complement(3215..>3428)
                     /locus_tag="VF357_10025"
     CDS             complement(3215..>3428)
                     /locus_tag="VF357_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7524530.1"
                     /translation="RLTGEAALEQMRRIAPAVPAILISGYDESGRIREIVAAGFSGFL
                     QKPFRRQDLGKKMRELLGVSDGPAAE"
BASE COUNT          630 a         1206 c         1132 g          460 t
ORIGIN      
        1 tcttgtccac cgcgatcccg gccacgaccg cgtacaggat caccggcagg caggggggga
       61 acaggagccc gagggacccg gacgaggtga gaaggccgag ggagaacctc tccgggtacc
      121 cctccacgag gagcgcgggc aacaggatcc cgcccagcgc cacgatcgtg accccggagg
      181 cgccggtgaa agtcgtgaag aaggtgcaca ccagcaccgc gaccacggcg atcccgccgg
      241 gcatccagcc gacgatcgcg cggaaaaagc gcaccagccg ccgggagacg tttccgaccg
      301 taagaacgta ccccgcgaag ccgaacagcg gaatggtggg aagcgtgggg gacgtcacga
      361 tccggtagat ctccgcggag atggacgcga gaggaacgtc ggcatggcgg aaaaagagga
      421 ccgcgaaccc gccaagcgcc gcgaagagtg gcgcgccgag gataacggcc gccgagagga
      481 tgatgatcga cgccgcgaac ggcacgctgt cgaggatcag ccccgaggag acgaacgcca
      541 cgccgacgac cgcgccggct gcggcggccg cccgcgtcca cacgcccccg ttcgcacgcc
      601 gcacgagtcg ccatgcgatg agggcgaaac ccgccggaat cacgccttcc gcaacccaca
      661 gggggaggaa cgaaacgacg gaatggcggt cggcccactc cgcccggacc agttggaggc
      721 ccgatccggc gagcaggagg gagacggccg cggaaaccgc cgcgacgaag gaggacagga
      781 cccgccggta cggctcccgg aaccggtccg cgagatgccc gatcgaaagg agccggtcgt
      841 cccgcgcggc gatcgccccg ccgagcaggc cgatccacag agtcgcgtgc tggacgaggg
      901 cactcgaacc ggggacgccc gttcgccaga cgcggcgccc gacgatctcg aggatcggga
      961 ggatcgccat gccgacgagg aggccgatcg aaacgccgtt ctcgacgcgg gccgcgagcg
     1021 tcgccggggg ccggtccccc gccagcgcga gttcgggagg ctgcgctgac tcagtgttgc
     1081 gattcataaa tacggcaacg tatcaagccg tggacgcagg gcgcagcgga gaggaagccc
     1141 ccccgccccg atacgagctt cgcgccgtcg gggtaccccc gcagttggaa acacgggggg
     1201 cacgagaccg aagtcctgta ccaaatggtc aaatgttacc ctgttcatga tctgaagcac
     1261 ttggtgtatg gtgccctagc ggcccgcgcc cttcgcaccg cgatgcgcgg cgcggtactc
     1321 gtcgcggaac ttcttcaccg tgtcgaacgc ctccgccggc atgatattcc cgcggatccg
     1381 cgggtaattg tcctccacca tcttccgcca ctgcgcctgg acctccggcg gcaccatgtg
     1441 gatcttgagg ccgttcttca ccatcacctc gagggcctcc tggttgaggc ggcggatctc
     1501 ggcgcgaagc cgcagccccg cccggcggga cgcctcgaga agtttcgggc gcagacccgg
     1561 cgggatcttc tcccacgccc gcttctcgat caccgtggcc cccgtcagcg gcgcgtaccg
     1621 aaggtccgcc atgtggggcg ccaggccgaa ccactggaag gccagcgcca ccagcggggt
     1681 gctggtgaac ccgttcacca tccccgtctg gaggccgggg agcaggtcat tcgtggaaag
     1741 ggggacgggg tgaaatcccg tctccttgta caactgcacg aggttcgtgt ccccctccca
     1801 ttggaagagt ttaagaccct tggcctccgc gggcgtgatc acgggagttt tcgaaaagaa
     1861 gtggacccac cccgcatcgc cccagttcag gacgacgaaa cccttctcct ccatcttccg
     1921 ctcgaggacc ggcgcgtacc ggtcccggac ggaatcgaac tcctcgtcgg attcgtacat
     1981 catcgggatg tgcaacgcat agaaggagag ctcgaggtat gccagcccga tgccggtgat
     2041 ggcggccgcc tggatctggc cgatccgcat cttccggatc atcgcatcct cgtcccccgc
     2101 gacgccgccc gggtagatgc gcagcgtaac ctccccgttg gacgccttcc tccagtcctc
     2161 acccatctcc tggagaaccc ggaaccacga cgatccctcc ggagccagcg tcgccatctt
     2221 gacgaccgtc ggcgccggca tcgcgcccac gggcgccgac aacgctgcta tcgccaccag
     2281 gagaaccgcc gccgaccgcc taatcgagga acagttcatc caccctccct ttcagccagc
     2341 gggcgcggcg ctgggtcacg aggttcccca tccggtactc cggcgccgcc gaccgcgcgt
     2401 cgaaggcgag cgcgcggtcg aggaggtcca ggaacccctt ccgatcctgg gtccgtaccg
     2461 acaccgtctc ggcgaaggtg accaacgggg agattttctt tccggcggac agcgccatcg
     2521 cccgctccat gtgatgccgc gcccgctcga ccgaccctcc catcgcctcg ggacgccccc
     2581 cctcgaaggc gacgaaaaat tcgtggatcg cgccggcgtc gtaccgctcg tccagcgcga
     2641 gcgcccttcg catcagcgcc tcgatcctcg ggaggtcggc cagcagcgat ggatcgtccg
     2701 acgacgtcga cacggcgagg ctccaggagg cgcccgtcca gtacagcagg ggaacgtctt
     2761 ccgcgtcgaa ccgcgcggcg gcggcggaag gatcctcgac cagcccggtg ggaaatccct
     2821 gccggccgac cgacaggcca cgcaccccgt actccctcgc tcggagcaag agcctgcggg
     2881 tacgctccat ccccgcccgg cgcgtcacgg gatcccgcgc ttcctcggcg tcctggcgaa
     2941 cgaaggcgac ggcatactgg gtgaatccct tggagagcgc cgtgaggagc cccttgtggt
     3001 ccggcgacga agccaggagc gattccatcg ccttgagagc gaagggaacc gcgtcgcgca
     3061 ccagttccgg gtcgtcgtct ctcgagaaga cctccccgcc ggaggaagcg gcgtcggcca
     3121 tccgggagac gacatacgac ctgggggaac aggcgagcag gaaacaggcc gccaggagca
     3181 ggggagaaaa ccgccggggg gggagtggca tccgtcactc cgccgcgggg ccgtccgaaa
     3241 cgccgaggag ctcgcgcatc ttcttcccga ggtcctgccg ccggaacggt ttctggagga
     3301 agccgctgaa cccggcggcg acgatctcgc ggattcgacc gctctcgtcg tacccgctga
     3361 tcaggatcgc gggcaccgcc ggagcgattc gccgcatctg ctccagggcg gcctcccccg
     3421 tcaggcgg
//
LOCUS       DASVJL010000485         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_556882, whole genome shotgun sequence.
ACCESSION   DASVJL010000485 DASVJL010000000
VERSION     DASVJL010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556882"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VF357_10030"
     CDS             <1..353
                     /locus_tag="VF357_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524531.1"
                     /translation="LKPGMTANVTIQVKKFENVLKIPNAAMRYRPVDKSKEPGGGTAG
                     KKKEPLGQRVYAIGKDGKLQAVPIKIGVSDGSTTELTGGNLKEGDFLVTGEAPKGSSR
                     SGGSPPGMSLGGFR"
     gene            363..1094
                     /locus_tag="VF357_10035"
     CDS             363..1094
                     /locus_tag="VF357_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7524532.1"
                     /translation="MSTATIELRGVTKTYLLGDVQVEALKGVDLSIATGEFLAVMGPS
                     GSGKSTLMNILGCLDRPTKGSYRLDGEEIGTLSRDGRAVIRRNKLGFVFQGFNLLPRL
                     SAQENVELPMVYDSTPASVRREKAHMALSSVGLGDRAHHLPNQLSGGQQQRVAIARAI
                     VNTPSLLLADEPTGNLDSATSDEIMGIFQRLNDEIGITLVLVTHEPDIALFARRVIRF
                     RDGNLVEDHPVVDRRRPPAAGGIPA"
     gene            1091..2317
                     /locus_tag="VF357_10040"
     CDS             1091..2317
                     /locus_tag="VF357_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7524533.1"
                     /translation="MNLLSAGKVSFRSLRANKMRSVLTMLGMIIGVAAVIVMVAIGAG
                     ANERIAAQIASVGSNLLLVLPGSTTSGGMRSGSGGTPTLTMDDAKAIGKELPAVALTS
                     PSSRSSGPVVYGNQNWSTILQGVSPEYFEMRDWKTIEGRYFTSQEMDAAVKVCLLGTT
                     VAENLFGSEDPVGKIVRIKRAPFTVVGVLESKGQSPNGQDQDDTVILPVTTLLKKLAG
                     SAHAGSIGVVMVQAVDGDQVKEAERTVTSLLRQRHRIGPGQDDDFSVRNLSEMLALAE
                     SATRIMSLLLGGIASVSLVVGGIGIMNIMLVSVTERTREIGIRMAVGARERDILSQFL
                     IEAVLLALTGGAIGIVLGATAAVLISRLAGWSTLISPAAALLAFGFSAAVGVFFGFYP
                     ARKASRMDPIEALRYE"
     gene            complement(2328..3014)
                     /locus_tag="VF357_10045"
     CDS             complement(2328..3014)
                     /locus_tag="VF357_10045"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEX7524534.1"
                     /translation="MRKIALFLLCFAALLASSGRIFAAGEGPGKWDESTPGRWESLPE
                     RHVGATDNGISVEVTISPGTGVSYRRVGRWAPGSVALRFGVDNVNAGKNDYLPGEAIF
                     PASVTFVFGDDSLKLGVKKRIGLFFKHLWYGFTPSGIRLTYAWGNGLPVGSMYRLWEE
                     ETVFVVAGPEEAGKTISSNRRLADDFSAAYGRPPKGPVTEVRVTARRPSREKKPVHAS
                     ITVRFPASPE"
     gene            complement(3007..>3289)
                     /locus_tag="VF357_10050"
     CDS             complement(3007..>3289)
                     /locus_tag="VF357_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524535.1"
                     /translation="VKPKVSGRRRSRVTLYAMAPESSYAGIMKAAMTAFPDVGIGSYP
                     MSDGEYRVRVVFRGDRFERTDACAARFTKLLSEIGYEVLRRAEERGEDA"
BASE COUNT          580 a         1111 c         1078 g          520 t
ORIGIN      
        1 tgctcaagcc cgggatgacg gcgaacgtca cgatccaggt gaagaagttc gagaacgtcc
       61 tgaagatccc caacgccgcg atgcggtacc gcccggtcga taaatcgaag gaacccggcg
      121 gggggacggc gggaaagaaa aaggagcccc tgggccagcg ggtctacgcc atcgggaagg
      181 acgggaaact ccaggcggtc cccataaaga tcggcgtttc cgacggctca accacggagc
      241 tcaccggggg gaacctgaaa gagggggact tcctggtgac gggggaggcg ccgaagggat
      301 cttcccgctc cggcggcagc ccgcccggga tgagcctcgg gggattcagg tgagggcctg
      361 acgtgtccac cgcgacaatc gagcttcgtg gggtcaccaa gacgtacctg ctcggggacg
      421 tgcaggtgga agccctcaag ggagtggacc tttccatcgc aacgggcgag ttcctcgccg
      481 tgatggggcc ctcggggtcg gggaagtcca cgttgatgaa tatcctcggg tgcctcgacc
      541 gacccaccaa gggcagctac cggctcgacg gcgaagagat cggaaccctg tcgcgggacg
      601 gccgcgcggt catacggcgc aacaagctcg gcttcgtctt ccaggggttc aatctcctcc
      661 cccgactctc cgcgcaggaa aacgtcgagc tgccgatggt ctacgacagc accccggctt
      721 ccgtgcgccg cgaaaaggcg cacatggcgc tttcctccgt cggcctgggc gatcgggcac
      781 accaccttcc gaaccagctc tccggcggcc agcagcaacg cgtggccatc gcgcgggcga
      841 tcgtcaacac gccgtccctc ctcctcgccg acgaacccac cgggaacctg gactcggcca
      901 ccagcgacga gatcatgggg atcttccagc gactcaacga cgagatcgga atcaccctcg
      961 tcctcgtcac tcacgagccg gacatcgcgc tgtttgcccg ccgggtgatc cggttccggg
     1021 acgggaacct cgtcgaggat cacccggtcg tggaccgtcg caggcccccc gccgccggtg
     1081 ggatccccgc atgaacctcc tctccgccgg gaaggtctcg ttccgatccc ttcgtgccaa
     1141 caagatgcgg tcggtcctga cgatgctggg gatgattatc ggcgtggcgg cggtcattgt
     1201 catggtcgcc atcggcgcgg gggccaacga gcggattgcc gcccagatcg cctccgtggg
     1261 ctcgaacctg ctgctcgtgc tcccggggag caccacttcg ggagggatgc ggtcgggctc
     1321 cgggggcact cctacactca cgatggacga cgccaaggca atcgggaaag agctccccgc
     1381 cgtggcgctg acttccccct cgtcccgcag cagcgggccg gtggtctacg ggaaccagaa
     1441 ctggagcacg atcctccagg gggtttcccc ggaatatttc gagatgcgcg attggaagac
     1501 catcgagggg agatatttca cctcacagga gatggacgcg gccgtgaagg tctgcctgct
     1561 gggcaccacc gtagccgaga acctcttcgg ctcggaggac ccggtgggaa agatcgtccg
     1621 gatcaagcgc gcccccttca ccgtggtcgg cgtgctcgaa tcgaaggggc agtccccgaa
     1681 cggccaggac caggacgaca ccgtgatcct acccgtcacg accctgctca agaaactcgc
     1741 cggcagcgcc cacgcggggt cgatcggcgt cgtaatggtg caggctgtcg atggagacca
     1801 ggtcaaggag gcggagcgga cggtgacctc cctgctccgc caacgccacc ggatcggccc
     1861 ggggcaggac gacgacttct ccgtgcggaa cctctccgaa atgttggccc tggccgagtc
     1921 ggccacgagg atcatgagcc tcctcctggg cgggatcgcc tccgtatcgc tggtggtcgg
     1981 cggcatcggg atcatgaaca tcatgctggt gtcggtgacg gagaggacgc gggagatcgg
     2041 gatccggatg gccgtggggg cgagggagcg cgacattctc tcccagttcc tgatcgaggc
     2101 ggtcctgctg gccctcacgg gtggcgcaat cgggatcgtg ttaggggcga ccgcggcggt
     2161 cctcatctcc cggctcgccg gctggtccac gctcatctcg cccgcggccg cgctcctcgc
     2221 cttcggcttc tccgccgcgg tgggcgtctt cttcggcttt tacccggcgc gcaaggcgtc
     2281 gcggatggac cccatcgagg cgctccggta cgaataaatg ttgccggtca ctccggcgaa
     2341 gccgggaacc ggaccgtgat ggatgcatga accggcttct tttcgcggga cgggcggcgg
     2401 gcggtcacgc ggacctcggt caccggcccc ttcggcgggc ggccgtaggc ggcactgaaa
     2461 tcgtcggcga gacggcggtt cgaggagatc gtcttccccg cctcctccgg tcccgcgacg
     2521 acgaacaccg tctcctcttc ccaaagacga tacatcgacc cgaccgggag accgttcccc
     2581 cacgcgtagg tcaggcggat cccggacggg gtgaacccgt accacaggtg cttaaagaag
     2641 agtccgatgc gctttttcac ccccagcttc aatgaatcgt ccccgaagac gaaggtgacg
     2701 gaggcgggaa agatcgcctc gccgggcagg tagtcgttct tcccggcgtt gacgttgtcc
     2761 acgccgaagc ggagcgcgac ggaacccggc gcccaccggc cgacgcgccg atacgatacg
     2821 cccgtgccgg gggatatcgt cacctcgacc gagatcccgt tgtcggtcgc ccccacgtgg
     2881 cgttcaggaa gcgactccca ccgccccggt gtggactcgt cccatttccc ggggccctct
     2941 cccgccgcga agatccgccc ggacgaagcg agcagcgcag cgaaacacag gaggaacagc
     3001 gcgatcttac gcatcttcgc cacgctcctc cgcccgacgg agaacttcat acccgatctc
     3061 cgagaggagc ttcgtgaagc gcgcggcgca cgcgtccgtc cgttcgaatc ggtcgccgcg
     3121 gaacaccacc cgcacgcgat actccccgtc gctcatcggg tacgacccga tgccgacatc
     3181 ggggaacgcc gtcatcgccg ccttcatgat cccggcgtag gacgattccg gggccatcgc
     3241 gtagagggtg acccggctcc tgcgccggcc ggaaaccttc ggcttcacc
//
LOCUS       DASVJL010000486         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1681277, whole genome shotgun sequence.
ACCESSION   DASVJL010000486 DASVJL010000000
VERSION     DASVJL010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681277"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VF357_10055"
     CDS             complement(<1..1072)
                     /locus_tag="VF357_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7524536.1"
                     /translation="MTRLAHGKRLRRIVAAGIVLIAGWALATAVVAANRYQLPQPTRS
                     APDSGGSAFDHGFSPADLYTLLKAGRPEGLLPNGNRVELSLNEELQAQIFDLFRRFDP
                     LYGVFAAMEPDTGRVVALVGYRRGGESDPWLPLKAIYPAASLIKVVTASAAIERGNVS
                     PQDEISYRGGIYTITRSGIHTRDGRGIPKMTLEEAIARSANAVFGKVAVNHVGGPVLE
                     EYLTKFGFGEKIPFDLPVETSHGQVPREEFELARTGAGFGEVYVSPLHMAMIMSAIAS
                     GGAMPRPVLIDRIENRDGTPLYESSSMKWRDTVLPETANAVVRMMVKTVEIGTSHRTF
                     GTAERTPLLHDMDVAAKTGSLSG"
     gene            complement(1069..>1671)
                     /locus_tag="VF357_10060"
     CDS             complement(1069..>1671)
                     /locus_tag="VF357_10060"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7524537.1"
                     /translation="GSQLQPFAGAGLSCFGFDPDPSLVAIARRKLAAYPKTRVETGSF
                     ADLPGLVPFPSDLLMCLGNSLVHVPQDEASRFFADAAGTLSRPGTLLLQILNYERLLR
                     EGVTELPMIRASEGTVEFRRKYEWEGDRKVLFRTSLRFAGADEPRIARNEVPLFPVYP
                     DELWEMLANAGFGDIRFYGDFARSEFSPGSEALVCLGRKA"
BASE COUNT          300 a          560 c          551 g          260 t
ORIGIN      
        1 acccggagag ggacccggtc ttcgccgcaa catccatgtc atgcaggagc ggggtccgct
       61 cggcggtacc gaacgtccgg tgcgacgttc cgatctcgac cgtcttcacc atcatccgga
      121 cgacggcatt cgccgtctcg gggaggaccg tgtcgcgcca tttcatcgag gaagactcgt
      181 acagcggcgt cccgtcgcgg ttctcgatcc ggtcgatcag gacggggcgg ggcatggcgc
      241 cgcctgaagc gatcgcggac atgatcatcg ccatgtggag gggggagacg tatacctcac
      301 cgaaccccgc gccggtgcgg gcgagctcga attcttccct cggaacctgt ccatggctcg
      361 tctctacagg gaggtcgaag gggatcttct cgccgaatcc gaacttggtc aggtactcct
      421 cgagcaccgg cccgccgacg tggttgaccg ccaccttccc gaacacggca ttggcggaac
      481 gggcgatcgc ctcctcaagg gtcatcttcg ggatccccct gccgtcccgt gtgtgaatcc
      541 cgctgcgcgt gatggtgtag atcccgccgc ggtagctgat ctcgtcctgg ggggaaacgt
      601 tcccccgctc gatggcggcg gaagcagtga ccaccttgat cagggaggcg gcggggtaga
      661 tcgccttcaa cggcagccac gggtcggact cgccgccccg gcggtagccg acgagggcga
      721 cgacgcgccc ggtgtccggc tccatcgcgg cgaacacgcc atacaggggg tcgaacctcc
      781 ggaacagatc gaagatctgc gcctggagct cctcgttcag ggaaagttcc acacggtttc
      841 cgttcggaag caggccttcc ggccgccctg ccttcaggag agtgtagaga tccgcggggg
      901 aaaatccgtg atcgaacgcg ctcccgccgg aatcaggcgc ggagcgcgtc ggctggggaa
      961 gctggtaacg gttcgcggcc acgaccgccg tcgccaacgc ccaaccggcg atcaggacga
     1021 tgccggccgc cacgatccgg cggagcctct tcccgtgtgc gagacgtgtc acgcttttct
     1081 ccccaggcac accaacgcct cggacccggg ggaaaattcg gatcgcgcga aatccccgta
     1141 gaaacggata tccccgaaac ctgcgttcgc cagcatctcc cagagttcgt cggggtagac
     1201 cgggaagagg gggacctcgt tccgcgcgat ccgcggctcg tccgcgccgg cgaaccggag
     1261 ggaggtccgg aagagcacct tgcggtcgcc ctcccattcg tacttccgcc ggaactccac
     1321 cgtcccttcg gacgcgcgaa tcatcggcag ctcggtcacc ccctcacgca gcagccgctc
     1381 gtagttcagg atctggagca gcagcgtgcc gggacgggaa agcgtccccg cggcgtccgc
     1441 gaaaaaccgc gacgcctcgt cctgcggaac gtggaccagc gagttgccga ggcacatcag
     1501 gagatccgag ggaaacggga caagacccgg caggtccgcg aacgatcccg tctcgacccg
     1561 ggttttcggg tatgcggcga gcttccgccg ggcgatggcg accagcgacg gatcgggatc
     1621 gaacccgaaa caggaaagac ccgcgccggc gaacggctgg agctgcgatc c
//
LOCUS       DASVJL010000487         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_87120, whole genome shotgun sequence.
ACCESSION   DASVJL010000487 DASVJL010000000
VERSION     DASVJL010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87120"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VF357_10065"
     CDS             <1..1117
                     /locus_tag="VF357_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7524538.1"
                     /translation="QLQDRGGLGHEKLMAARNQLLGFAGKEPRLTRVRPNGLEDVPQY
                     RIDVDLDKGGTLGIPVDAINNTISASFGGAYVNDFIQGGRVKRVYVQADAPYRMLPND
                     LGRLYVRNSAGTMAPFSSFASGHWFQGSPRLERFNSFPSMNIWGEAPRGKSSGEAMQA
                     MEEIVSKLPKGIGFDWTGLSFQERMAKSQAPLLFAFSIIVIFLCLAALYESWIVPITI
                     LLALPLGVIGGVVATSLRGLPNGVYFQIGLLTVLGLTTKNAILIVQFAMAKVDEGAGL
                     IDATLEGARLRFRPIVMTSLAFGFGVLPLAMANGAGAGAQVAIGTGVLGGMVTATFLA
                     IFFIPLFFVGVVQLFEKKKGVRAEGSGPDAESPSEGR"
     gene            1120..2529
                     /locus_tag="VF357_10070"
     CDS             1120..2529
                     /locus_tag="VF357_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7524539.1"
                     /translation="MMERLLSLLAITVVLLTGCTLAPKYTRPEAPVPASWPSGPAYDN
                     AQVSPGALAATEVQWREYFTDERLRKIIEAALQNNRDLRIAALNVERARAVYGIQRAE
                     LFPTVNAAGSGSKQRVPTDLSTTGEAATVTQYGVNLGIASWEIDFFGRIRSLKDAALQ
                     EYLATEQARRGAQILLVSGVANAYLTFTADRENLKLAQSTLGTQRAAYNLIRRRYDVG
                     LSTELDLRQAQTRVDAARVDMALYSRLAAQDENALDLLVGSKVPAGLLSDDLTLASPM
                     REVSPGMSSEVLLLRPDILQAENLLKAANANIGAARAALFPRISLTTAFGTASSELSG
                     LFKSGSESWNFSPQIVLPIFDARAWSALQAIKVEREIVLAQYEKAIQTAFREVADALA
                     VRGTVEDQLSAQQSLVEAASETYRLSNMRYTKGIDSFLGVLDAQRSLYAAQQGLIAVR
                     LTRIANQVRLYAVMGGGSR"
     gene            2695..>3473
                     /locus_tag="VF357_10075"
     CDS             2695..>3473
                     /locus_tag="VF357_10075"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7524540.1"
                     /translation="MIPRTWITTVFSIIAIVVFGVSARAADNAAVPPATSPEGIVTWS
                     LVDVTSTALKNHPLILQSDADVAAAVARKGQAQSAWYPSVNLSTGYARVRALNTSTNQ
                     NVTTQNEFGRGDLNWMLYDFGRTGASVDRADANAAISRENAATTREDVVFVATVAFYN
                     VLRAEKTLEFQRENLRQQESLYRQASAFYEAGVRAKIDVVRAEANVYDVRAQLSQAEN
                     GLRVARITLLQRIGVDGPAGFQLSGTNGKSTTTALVGHILKT"
BASE COUNT          624 a         1109 c         1124 g          616 t
ORIGIN      
        1 ccagttgcag gaccggggcg ggttgggcca cgaaaagctg atggcggccc gcaaccagct
       61 gctcggcttt gccggaaagg agccgagact gacccgtgtc cgtcccaacg ggctggaaga
      121 cgtcccccaa taccggatcg atgtggatct ggacaagggg ggcaccctgg gaattcccgt
      181 cgacgccatc aacaacacca tctcggcatc gttcggcgga gcctacgtga acgacttcat
      241 ccagggggga agggtcaaac gggtatacgt ccaggccgat gcgccgtacc gcatgctgcc
      301 gaacgacctg ggccggctct acgtccgcaa cagcgcgggg acgatggcgc cgttctcttc
      361 cttcgcctcc ggccattggt tccagggatc gccacggctg gaacgtttca acagcttccc
      421 gtcgatgaac atctggggtg aagcgcctcg cggcaagagt tcgggggagg cgatgcaggc
      481 catggaggag atcgtctcga agctacccaa ggggatcggc ttcgactgga ccggactctc
      541 cttccaggag cggatggcca agtcccaagc cccgctgctc ttcgcctttt ccatcatcgt
      601 gatcttcctc tgtctggcgg ccctttacga gagttggatc gttcccatca caatcctgct
      661 ggcattgccg ctcggggtca tcggcggcgt ggtggccacg tcgcttcggg gactgcccaa
      721 cggcgtgtac ttccagatcg ggctcctcac cgtcctgggg cttacgacca agaacgccat
      781 cttgatcgtt cagttcgcca tggccaaggt ggacgaggga gcggggttga tcgatgccac
      841 gctggagggg gcgagattga ggtttcgccc gatcgtcatg acctcgctgg ccttcgggtt
      901 cggcgtcctt cccctggcca tggccaacgg ggccggcgcg ggagcgcagg tggccatcgg
      961 caccggcgtc ctgggcggga tggttaccgc caccttcctg gcgatcttct tcattcccct
     1021 tttcttcgtg ggggtggtcc aactgttcga aaagaagaaa ggcgtccggg cggaaggttc
     1081 cggtccggac gccgagagtc cttcggaggg gcgctaacga tgatggagag gctgctttca
     1141 ctcctggcga taacggtcgt ccttctcacc ggctgcacgc tggcgccgaa atacacccgg
     1201 cccgaggcgc cggttcccgc ctcctggcca agcggtccag catacgataa cgcacaggtc
     1261 tcgcccggcg cgctggcggc gaccgaggtt caatggcggg aatatttcac cgacgagcgg
     1321 cttaggaaga tcatcgaggc ggcgttgcag aacaaccgcg accttcggat cgccgccttg
     1381 aacgtcgaga gggcgcgcgc ggtgtacggc atccagcggg ccgaactttt cccgacggtc
     1441 aatgcggccg gcagcggaag caagcagcgt gtgcccaccg atctttcgac caccggggaa
     1501 gcagcgactg tcacgcagta cggcgtcaac cttgggatcg cttcctggga gatcgacttc
     1561 ttcggccgca tccggagcct gaaagatgcc gcgctgcagg agtacctcgc caccgagcag
     1621 gcccgcagag gcgcgcagat cctgttggtg tccggagtcg ccaacgcgta cctgaccttc
     1681 accgcagacc gggaaaacct caagctggct caatccaccc tgggtacaca acgggccgcc
     1741 tataatttga tccggaggcg ttacgacgtc gggctctcga ccgagctgga tcttcgccag
     1801 gcgcagacga gagtggatgc ggcccgggtg gatatggccc tctactcccg attggcggct
     1861 caggacgaaa acgccctgga ccttctggtc ggatcgaagg tgccggccgg gctgttgtcg
     1921 gacgacttga ccctcgcaag ccctatgagg gaggtttctc ccggaatgtc gtccgaggtg
     1981 ctccttcttc gccccgatat ccttcaggcg gaaaatctgc tcaaggccgc caacgccaac
     2041 atcggtgcgg cccgcgcggc cctcttcccc cgcatttcac tgacgaccgc gttcgggacg
     2101 gcgagcagcg agctgtcggg actgttcaaa tccggttcgg aaagctggaa cttctcgccg
     2161 cagatcgttt taccgatttt cgacgcccgc gcatggtcgg cgctgcaggc gatcaaggtg
     2221 gaaagggaga tcgtcctggc ccagtacgag aaggccatcc agacggcttt cagggaagtg
     2281 gccgacgccc ttgctgtgcg aggcacggtg gaggaccagt tgtcggcgca gcaatccctg
     2341 gtcgaagccg catcggagac ctatcgtctt tcaaacatgc gttacacaaa gggaatcgac
     2401 agcttcctgg gtgtcctcga cgcgcaacga tcgctctatg cggcgcagca ggggctgatc
     2461 gccgtccgcc tgacgaggat cgccaaccag gtgaggcttt acgccgtgat gggtggggga
     2521 tcccgataaa caggaccgac gagttcgcct gctgccgcga caacccggag cggcacgaga
     2581 tgccggcgcg gctgccggat atgcctccgc cccttgacag cggcgcggcg gtcctcctac
     2641 gatggggcga tgccgactga gtgcccgctc ggcatcattt cccgggagga cgccgtgata
     2701 ccacgcacct ggatcacaac cgttttttcg attattgcga ttgtcgtgtt cggcgtctcc
     2761 gcccgcgcag ccgacaacgc ggccgtcccg cccgcgacct cccccgaagg gatcgtgacg
     2821 tggtcactcg tcgacgtaac gtcgacggcc ctcaagaacc acccgctgat cctgcagtcc
     2881 gatgcggacg tcgccgcggc ggtggcgaga aaggggcagg cgcagtcggc ctggtatcca
     2941 tcggtcaatc tgtccaccgg ctacgcccgc gtgcgggccc tcaacacctc gacgaaccag
     3001 aacgtcacga cgcagaacga gttcggccgc ggagacctca actggatgct gtacgacttc
     3061 gggcggaccg gcgcctcggt cgaccgcgcc gacgcgaacg ccgcgatcag tcgcgagaac
     3121 gccgccacga cgcgggagga cgtggtcttc gtggcgacgg tcgcgttcta caacgtcctg
     3181 cgtgcggaaa agacgctgga gttccagagg gagaacctcc ggcagcagga gtcgctctac
     3241 cggcaggcgt ccgccttcta cgaggccggc gtccgtgcga agatcgacgt ggtccgggcc
     3301 gaggcgaacg tgtatgacgt gcgtgcccag ctgagccagg cggagaacgg actccgggtc
     3361 gcgcggatca cgctgctgca gcggatcggt gtcgacggcc ctgcggggtt ccagctctcc
     3421 ggcaccaatg gcaaatcgac caccacggct ctggtcgggc atattctcaa aac
//
LOCUS       DASVJL010000488         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_463345, whole genome shotgun sequence.
ACCESSION   DASVJL010000488 DASVJL010000000
VERSION     DASVJL010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463345"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VF357_10080"
     CDS             <1..414
                     /locus_tag="VF357_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7524541.1"
                     /translation="RRKNDELTALNRDLERRVVERTEALTTQNRLLTESLGILDNLPM
                     AIVGMDPQGTVFQCNRKGMVCFGSRIGPVIGANRRDVLTEAQNRLVDEVLEKSLATGN
                     GSASAVDGKHQAVLERNPYAPDCVILIVHEEPVGG"
     gene            407..1348
                     /locus_tag="VF357_10085"
     CDS             407..1348
                     /locus_tag="VF357_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7524542.1"
                     /translation="MDDAVLLVDDEANILNALTRLFLDRNVRVLRAGNGEEALGIVRR
                     EPVAVVVSDNLMPGMRGVELLSRVRDCSPDTVRVLLTGYADLPTAIEAINRGEVFRFH
                     VKPWVDEEIVRTVEEGVRRYQVVRSLRHGDEAALRSIAQTIELKDPYTRGHCDRVAAF
                     ALTIAEALHLPEGTRRAIKHGSWLHDCGKIGVPETILNCPGKLSAADFEVVKKHPGWG
                     ADVGRQANLPQEVINIILYHHERFDGRGYPTGAKGTEIPLEARIVAVADIFDAMSTDR
                     PYAKGYERAEVMRVLGVLRGAALDPQLVDIFLAGLTP"
     gene            1345..1998
                     /locus_tag="VF357_10090"
     CDS             1345..1998
                     /locus_tag="VF357_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding-motility protein MglA"
                     /protein_id="HEX7524543.1"
                     /translation="MIEYDSEEQRMVLKIVYYGPALSGKTTNLLRMHDLLAKEGRGDL
                     IVLDTKDDRTIFFDLLPFFLVAPSGLKIKVKVYTVPGQVQHDATRKAVLQRADGVAFI
                     ADSRRSEAKNNTESFGNLERNLSIVGIDIDKLPLVIQHNKRDLPDIIPEEEIRRAWGP
                     TGFPVILSSALEGWGVMETFRALAERAYDALNRRYDFAGTHGLTRESFAERLVRRPG"
     gene            2015..>2374
                     /locus_tag="VF357_10095"
     CDS             2015..>2374
                     /locus_tag="VF357_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524544.1"
                     /translation="MPPDIEGRGRIVYCVGEERALRDVLPESEAAALLAAASRAGLRG
                     VRVVDPGGKALWGSGDDVPPPGEGDPRRPILLEGEPAGSVVLPAGAAGGETQDALLAL
                     LADTLTAMAHNNLKRMLT"
BASE COUNT          407 a          773 c          869 g          325 t
ORIGIN      
        1 cggcggaaga acgacgaact gacggcgctc aaccgggacc tcgagcggcg cgtggtggag
       61 cgcacggaag cgctgacgac gcagaaccgg ctgctgacgg agtctctcgg gatcctcgac
      121 aacctcccga tggcgatcgt ggggatggac ccgcagggaa ccgtcttcca gtgcaaccgg
      181 aaggggatgg tctgcttcgg aagccgcatc ggaccggtga tcggggcgaa tcgccgcgac
      241 gtcctgaccg aggcccagaa ccggctggtg gacgaggtcc tcgagaagag cctggcgacg
      301 gggaacggga gcgcctccgc cgtcgacggg aagcaccagg cggtcctcga gcggaacccg
      361 tacgcccccg actgcgtgat cctcatcgtg cacgaggagc ccgtcggtgg atgacgccgt
      421 cctcctcgtc gacgacgagg cgaacatcct gaacgcgctg acgcgcctgt tcctggaccg
      481 caacgtgcgg gtcctgcgcg ccgggaacgg ggaggaggcg cttgggatcg tgcggaggga
      541 gccggtggcg gtcgtcgtct ccgacaacct catgccgggg atgcgcggag tcgagctgct
      601 ctcgcgggtg cgggattgct cgcccgacac cgtgagggtc ctcctgacgg gatacgccga
      661 cctgcccacc gcgatcgagg cgatcaaccg gggagaggtg ttccgcttcc acgtgaagcc
      721 gtgggtggac gaggagatcg tccgcaccgt cgaggaggga gtgcggcggt accaggtggt
      781 gcgctccctg cgccacgggg acgaagccgc cctgcgctcg atcgcccaga cgatcgagtt
      841 gaaggacccg tacacccggg gacattgcga ccgcgtggcg gcgttcgccc tgacgatcgc
      901 ggaggcgctg cacctcccgg aggggacgcg gcgggcgatc aagcacggga gctggctcca
      961 cgactgcgga aagatcggcg tgccggagac gatcctcaat tgcccgggga agctgtccgc
     1021 ggcggatttc gaggtggtca agaagcatcc ggggtggggc gcggacgtcg ggcggcaggc
     1081 gaacctcccc caggaggtca tcaacatcat cctctaccac cacgaacggt tcgacggccg
     1141 ggggtacccc acgggcgcga aggggacgga gatcccgctc gaggcgcgga tcgtcgcggt
     1201 ggcggacatc ttcgacgcca tgtcgacgga ccgcccctac gccaaggggt acgagcgggc
     1261 ggaggtgatg cgcgtgctgg gggtcctccg gggagccgcc ctcgatccgc aactggtcga
     1321 catcttcctg gcgggcctca ccccatgatc gaatacgact ccgaagagca gcgaatggtc
     1381 ctgaagatcg tctactacgg gcctgccctc tccggaaaga cgacgaacct gctgcggatg
     1441 cacgacctgc tcgcgaagga aggacgggga gacctgatcg tcctggatac gaaggacgac
     1501 cggacgatct tcttcgacct cctccccttc tttctggtcg cccccagcgg gctgaagatc
     1561 aaggtgaagg tgtacaccgt cccggggcag gtgcagcacg acgccacccg gaaggccgtg
     1621 ctccagaggg cggacggggt cgcgttcatc gccgattccc gccggtcgga ggcgaagaac
     1681 aacacggaga gcttcgggaa cctcgagcgg aacctctcca tcgtcgggat cgacatcgac
     1741 aagctcccgc tggtgatcca gcacaacaag cgcgacctcc ccgacatcat cccggaggag
     1801 gagatccgcc gggcctgggg gccgacggga ttccccgtca tcctttcctc cgcgctcgag
     1861 gggtggggcg tcatggaaac gttccgggcg ctggcggagc gcgcctacga cgccctgaac
     1921 cgccggtacg atttcgccgg cacgcacggg ttgacgcgcg agtcgttcgc cgagcggctc
     1981 gtccgccggc caggatagcg gagaggggga agggatgccg ccggacatcg agggccgcgg
     2041 acggatcgtc tactgcgtcg gggaggaacg agccctgcgc gacgtcctgc ccgagtccga
     2101 agccgcggcg ctgctcgcgg cggcatcccg ggcgggtctt cgcggcgtgc gcgtcgtcga
     2161 cccgggcggg aaggccctct ggggaagcgg ggacgacgtc ccgccgccgg gggaggggga
     2221 cccgcggcgc cccatccttc tcgaggggga accggcgggc agcgtcgtcc tgccggccgg
     2281 ggcggccggg ggagagacgc aggacgccct cctcgccctg cttgcggaca ccctcacggc
     2341 gatggcgcac aacaacctga agcggatgct cacc
//
LOCUS       DASVJL010000489         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_182579, whole genome shotgun sequence.
ACCESSION   DASVJL010000489 DASVJL010000000
VERSION     DASVJL010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182579"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VF357_10100"
     CDS             <1..756
                     /locus_tag="VF357_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX7524545.1"
                     /translation="DELFCGYPRYAWVRQGNVVRGIPGFLRRPLCSLLSRVPIHKVQR
                     GAESVLYDDPAEMYFHTVGIFERRRLGEIVPGVVGDAHLPYFRTFRDPRCGGIVERAM
                     ATDIKTYLVDDILTKVDRASMAYSLEARVPLLDHRIVEFAARLPMEHKAHRGETKHLL
                     RRILYRHVPRKLIDRPKMGFGIPVNRWLRKELRPLLDEYLGEDRVRREGFLRPEGVER
                     VVREHLSGRRDHQYRLWALLVFAMWVERYRPTS"
     gene            complement(801..1550)
                     /locus_tag="VF357_10105"
     CDS             complement(801..1550)
                     /locus_tag="VF357_10105"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX7524546.1"
                     /translation="MMAVAAFLVGACIGSFLNVVIYRLPRGESIVSPRSRCPGCGRAI
                     RAWENIPILSFIVLGGKCAGCGGSISWRYPAVELLTATGFAAIFLLDAPGIPLLRDLL
                     FFSLLVPIAFIDIDHRIILDELSLGGLAAGLLLSFLPGGEWKGSVAGALLGGGILYAT
                     AFLYEKVRGAEGMGGGDIKLLAMIGAFVGWRGTLATIFFGALLGAAGGILAMRKGGEG
                     LKTAIPFGPYLCVAALLSRYLGGWFWGMMRI"
BASE COUNT          317 a          580 c          549 g          253 t
ORIGIN      
        1 gacgaactgt tctgcgggta cccgcgttat gcgtgggtcc ggcaggggaa cgtggtgcgg
       61 gggatcccgg ggttccttcg gcggccgttg tgttccctcc tttcccgcgt cccgatccac
      121 aaggtacagc ggggggcgga gagcgtcctg tacgacgacc cggcggagat gtacttccac
      181 accgtgggga tcttcgagcg aaggcgcctc ggagagatcg tcccgggggt ggtcggcgac
      241 gcccatcttc catacttccg cacgttccgg gatccccgct gcggggggat cgtcgagcgc
      301 gcgatggcca ccgatatcaa gacgtacctg gtggacgaca tcctaaccaa ggtagatcgg
      361 gcgtcgatgg cgtactcgct ggaggcgcgg gtacctctgc tcgaccaccg gatcgtggag
      421 ttcgcggcac gactccccat ggagcacaag gcgcaccgcg gagaaacgaa gcatctgctg
      481 cggaggatcc tctaccggca cgtgccgcgg aagctgatcg accgcccgaa gatggggttc
      541 ggcatccccg tcaaccggtg gctgcgcaag gaactgcggc cgctgctcga cgagtatctg
      601 ggtgaagacc gcgtgcggag ggaggggttc ctccgaccgg agggggtgga gcgggtcgtc
      661 cgggagcacc tgtcgggtcg ccgggaccac cagtaccggc tgtgggcgct gctggtcttc
      721 gcgatgtggg tggagcgcta ccgcccgacg tcttgaccca agggaccttg cctatccgca
      781 ctgggaaaac gcctcgccgt tcagatccgc atcatccccc aaaaccatcc gccgagatac
      841 cgggacagga gggcggcgac gcacaggtaa ggcccgaacg gaatcgccgt cttcagcccc
      901 tccccgccct tgcgcatcgc gaggatcccc cccgccgcac cgaggagcgc accgaagaag
      961 atcgtcgcga gggtgccacg ccacccgacg aaggcgccga tcatcgccag cagttttatg
     1021 tccccgccgc ccatcccctc ggccccccgc actttttcat acaggaaggc cgtcgcgtag
     1081 aggatgccgc ctcccagcag cgcacccgcg acgctcccct tccactctcc gccgggaagg
     1141 aaggaaagga gcagtcccgc ggcgagccca ccgagggaga gttcgtcgag gatgatccgg
     1201 tggtcgatat cgatgaaggc gatcggaacg aggagagaga agaagagcag atcgcgcaac
     1261 agcggaatcc cgggcgcgtc cagtagaaag atcgcggcga acccggtcgc cgtgaggagc
     1321 tcgaccgccg ggtaccgcca ggagatcgat cccccgcacc cggcgcattt ccctccgagg
     1381 acgatgaagc tcaagatcgg gatattctcc cacgcccgga tcgccctgcc gcagccgggg
     1441 cagcgcgaac ggggcgacac gatcgactcc cctcgcggca accggtaaat caccacgttc
     1501 aggaaagatc cgatgcacgc gccgacgagg aacgccgcta ccgccatcat gaccaacccg
     1561 acaacccctt tcccgcgagg atctccgcgt agagccgctc ctgcgcccgc accatctcgt
     1621 cctggtcgaa ctcccggacc aggcgctccc gctttctcgt cacatccagc ggaagccgtc
     1681 ccgccaggat gtccgagac
//
LOCUS       DASVJL010000490         9098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1590823, whole genome shotgun sequence.
ACCESSION   DASVJL010000490 DASVJL010000000
VERSION     DASVJL010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590823"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1610)
                     /gene="cimA"
                     /locus_tag="VF357_10110"
     CDS             complement(21..1610)
                     /gene="cimA"
                     /locus_tag="VF357_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX7524547.1"
                     /translation="MKTIYLYDTTLRDGTQSEEISLSVLDKVAIAEKLDEFGIHYIEG
                     GYPGSNPKDKEFFERARKMRWKNSVLCAFGMTRRVGKKVDEDSNMKALVAAGTPVITV
                     VGKAWDFHVIEALHTTLEENLHAVKDTVEYLKKNADKVFFDAEHFFDGYVHNPKYAMK
                     VLAAAVDAGADVLVLCDTNGGSLPSDISRIVKEVRKATATAIGIHTHNDGEMAVATTL
                     AAVESGATQVQGTINGYGERCGNANLCSILPALELKMGREVLPKGQLRYLTDLSRYVA
                     EVANVGQWLHAPYVGNAAFAHKGGMHVSAIRRHTKTYEHVEPETVGNHRRVLISDLSG
                     RSNILSKIQETGLKFKANDPNTEKILDEIKDLEHKGFQFEGADASFELLARRAMGEHE
                     PFFELVGFRVIDEKRSEGEAPIAEATIQISVDGKAEHTAALGNGPVNAMDNALRKALE
                     KFYPEVAEVKLLDYKVRVIRQGGTGSSVRVLIKSGDRDEIWGTVGVSHNIIEASWQAL
                     VDSIRYKLWRTRRADSGEQGE"
     gene            complement(1660..2880)
                     /locus_tag="VF357_10115"
     CDS             complement(1660..2880)
                     /locus_tag="VF357_10115"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005155.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX7524548.1"
                     /translation="MAIIVQKYGGTSVGTVEKIKNVAKRVARTKDQGNDVVVVVSAMS
                     GETNRLLGLANQISEMPNERELDVVAATGEQVTIGLLAIALTEMGCKAKSFCGFQIPV
                     LTDAAYVKARIRQIRGETITKALKDGYVAVVAGFQGIDDSGSITTLGRGGSDTSAVAV
                     AAALKADVCEIYTDVDGVYTTDPNICADARKLDKISFEEMLELASLGAKVLQIRSVEF
                     GMKYGVRIHVRSSFNDNQGTIVTTEEEIMETAVVSGVAYSKSEAKITVVKVPDRPGIA
                     AKIFKPLSEANIVVDVIVQNVSVTGFTDLTFTIGRTDYKKAMQITSMAAKDVGAERVV
                     GDDKIAKVSIVGMAMRSHSGVALKVFETLAAEGINILGISTSEIKISVLIEEKYTELA
                     VRVLHGAFGLGNPA"
     gene            complement(2889..3368)
                     /gene="tsaE"
                     /locus_tag="VF357_10120"
     CDS             complement(2889..3368)
                     /gene="tsaE"
                     /locus_tag="VF357_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX7524549.1"
                     /translation="MILLLHSGSPEDTLAIARALGAALRPGDVVALYGDLGAGKTLFC
                     KGVGEALGIPPDRIVSPTFTIVTEHAGPVPLTHIDLYRLAGVQEADEIGMRELLSGDG
                     VCLVEWAEKIEELLPTDCIQVKFSISGDDRREIAIAAPDLPGFHDFRARSLRFHPGG"
     gene            complement(3365..4945)
                     /locus_tag="VF357_10125"
     CDS             complement(3365..4945)
                     /locus_tag="VF357_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX7524550.1"
                     /translation="MKIVTARQMAELDRVTIHTYGIPALVLMENAGRSCTDRIFRILE
                     EKAGGPQEASVAVVCGKGNNGGDGMVIARHLHNRGAYVEVFLLCEEKDLSTDARTQHE
                     ILRRMDVEIRVIRDTEGVEDLRTYLEEVHLCVDAILGTGLSSPLSGIVREVVEVINLS
                     MAPVFAVDIPTGIDATTGRIMGEAIRAESTGTFGLLKLGHVLLPGSIHCGETEIYDIG
                     IPPRAVFDAQIKTEALDEQVVKSMLSVRPPDFHKGDAGRVHIVGGSPGMTGAPCLAGS
                     AALRMGAGLITVVTPESLRPIVEAKQMEVMTRGIPDGGTGYFAPDMIPALMEVLSKAD
                     MIVVGPGLGMTDTMPAFVKELISRIKVPFLLDADALNALSGEAVTLQEATAPCILTPH
                     PGEMARLMKETIESIEATRIDSARHLAEEERVTVILKGARTIVATPKGDIFINTTGNP
                     YMASGGMGDALTGMIAALASQGLSPNDAACAGVFLHGMSADLLVRDHPMTPVTATEVI
                     DNIRGALQHTLGEPQPEE"
     gene            complement(4946..5335)
                     /gene="acpS"
                     /locus_tag="VF357_10130"
     CDS             complement(4946..5335)
                     /gene="acpS"
                     /locus_tag="VF357_10130"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX7524551.1"
                     /translation="MIAGIGVDIVDIARIQTLLDLYGERFLRRVYTEAETAYAMSGAN
                     KAERLAGRFAVKEAMLKALGTGKSQGILWKDVETVRGRFGKPEVHLHGQAVKWAKWRR
                     GSQVHASITHDGGKAVAFVILEKAGEE"
     gene            complement(5346..6077)
                     /locus_tag="VF357_10135"
     CDS             complement(5346..6077)
                     /locus_tag="VF357_10135"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX7524552.1"
                     /translation="MTLTRKRLGVNIDHVATLRQARRGQVPEPAVAASIAEQYGADGI
                     TVHLREDRRHIQDRDLEVLMRVVTTRINLEMAAIDEITRIACGLKPYSCTLVPEKREE
                     ITTEGGLDVLGYRESMVQTTERLKNAGIVVSMFIDPELPQVRASRQAGADAIEIHTGT
                     FCEAFGAGKHDAELAKIRSAAAFGASIGLKVFAGHGLDVRNIVPILGIPEIEEFNIGH
                     SIIARAVFLGLPAAVKEIADLIHGA"
     gene            complement(6074..7423)
                     /gene="glmM"
                     /locus_tag="VF357_10140"
     CDS             complement(6074..7423)
                     /gene="glmM"
                     /locus_tag="VF357_10140"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF008139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX7524553.1"
                     /translation="MRRLFGTDGVRGVANTDPMTVETALALGQAATYLFRGKNGRHKI
                     VIGKDTRLSGYMFETALSAGICAMGGDVLLVGPMPTPGIAFLTHSMRADAGVVISASH
                     NPYPDNGIKFFGGDGFKLPDDVEDRIESLMYGEHLKENRPPSPQIGKAQRIDDATGRY
                     IVYLKSTFPTNLSLVGLRIVVDCANGAAYRIAPQVFAELGAEVIPIGVSPNGVNINDQ
                     CGSLYPEVVSAKVREARADIGISLDGDADRVIIVDQKGEVQDGDRIMAICAGEMARKK
                     RLSKNTVVATVMSNIGLELYLKERKIKLLRAPVGDRYVVEAMRAGKYNFGGEQSGHLI
                     FLDHATTGDGVLAALQLLAVMVESGKKISELGKGLVVFPQMLHNIRMNHRVPLESMKG
                     FQKAQAEFEKALGSRGRIVVRYSGTEPLLRIMVEGENLDEVEAIVKALGEKAREEAR"
     gene            complement(7437..8105)
                     /locus_tag="VF357_10145"
     CDS             complement(7437..8105)
                     /locus_tag="VF357_10145"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEX7524554.1"
                     /translation="MLPVDLFRILRRFASQNLWLKVLALALAVAAWWFVAGENKVLVS
                     FTIPLEIRNVPKGMTVTNKAERQVEVRLSGPSSLLSGMRPSEIFAAVDLTPARTGRQS
                     FTLDDRAVKVPPGIKVQRIYPSSIEVILDRTERRALKVSVRIGGGAAVRKRVAKVEID
                     PASVEVEALPEEFARMPVVYTEEVVPDRTDGAYSAIARVETREPHAKIVGNPNVRVKI
                     QFRK"
     gene            complement(8092..8868)
                     /gene="cdaA"
                     /locus_tag="VF357_10150"
     CDS             complement(8092..8868)
                     /gene="cdaA"
                     /locus_tag="VF357_10150"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX7524555.1"
                     /translation="MVGGLLSIGAIDVLDILLVAFIIYWVLLFIRGTRAVQMLFGLLI
                     LMVMYVVAKKAGMVTFQWLVGNFLENLLVVLVVVFQSEIRRGLAKIGQWRLFGGRGSV
                     PDPDMIDQIAQCAFLLAGERTGAILLLERETGLEELVEHGKKLDALFSHELAASIFAT
                     SSPVHDGAVVIRRNRIAAAGVILPIPAESVVTRGMGTRHRAAFGVASETDAVAVVVSE
                     ETGNVTVFSNRSANRTETVQQLRDTLGLLFRMEVTPLAPG"
     gene            complement(8862..>9098)
                     /locus_tag="VF357_10155"
     CDS             complement(8862..>9098)
                     /locus_tag="VF357_10155"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX7524556.1"
                     /translation="HRHLDRLRILERPIVFGPSRKSFIGRLTGAPPAERSFGTAAAVA
                     AAVLRGAHVVRVHDVKEMREVVEVAYAIRGEASW"
BASE COUNT         1436 a         3235 c         2698 g         1729 t
ORIGIN      
        1 cggcgcgtcg cttcccgccg ctactctccc tgctcccctg aatcggcacg ccgtgtcctc
       61 cacagtttgt accggatgct gtccaccagg gcctgccagg acgcctcgat gatgttgtgc
      121 gacacgccca cggtgcccca gatctcgtcc ctgtcgccgg atttaatcaa gacgcgtacc
      181 gaggaaccgg tccctccctg ccggatgaca cggaccttgt agtcgaggag tttcacctcg
      241 gcgacttcgg ggtagaattt ctccagcgcc ttccgcaagg cgttgtccat ggcgtttacc
      301 gggccgttcc ccagcgccgc cgtatgctcc gcctttccgt cgacggagat ctggatcgtc
      361 gcctcggcga tcggcgcctc gccctcggac cgtttttcgt cgatcacacg gaagccgacc
      421 agttcgaaaa acggctcgtg ttcccccatc gcccggcgcg ccagcaactc gaacgaggcg
      481 tccgccccct cgaactggaa tcccttgtgc tcgaggtcct ttatttcgtc gaggatcttt
      541 tcggtgttgg ggtcgttcgc cttgaacttg agacccgttt cctggatctt cgagaggatg
      601 ttgctgcgcc cggaaaggtc ggagatcagg acccgccggt ggtttcccac cgtctcgggt
      661 tcgacgtgct cgtacgtctt cgtgtgccgg cggatggcgg agacgtgcat ccctcccttg
      721 tgcgcaaacg ccgcgttccc gacgtacggc gcgtggagcc attgcccgac gttggccacc
      781 tcggccacgt agcgtgacag gtcggtcaga taccgcagct gtcctttcgg aaggacctcc
      841 cgccccatct tgagctccag cgcggggagg atggaacaca agttcgcgtt cccgcaccgt
      901 tccccgtacc cgttgatcgt cccctgcacc tgggtggcgc cgctctccac ggcggccagc
      961 gtagtggcca ccgccatttc accgtcgttg tgcgtatgga tgccgatcgc cgtggcggtc
     1021 gccttgcgca cttccttcac gatccgcgat atgtcggacg ggagcgatcc gccgttcgtg
     1081 tcgcaaagga ccaggacgtc ggcccccgcg tcgaccgcgg ccgccagcac cttcatcgcg
     1141 tatttcgggt tgtgcacgta cccgtcgaag aaatgctcgg cgtcgaagaa aaccttgtcg
     1201 gcgtttttct tcaggtactc caccgtgtcc ttgacggcgt gcaggttctc ctcgagggtg
     1261 gtgtgcagcg cctcgatgac atggaagtcc cacgccttcc ccaccaccgt gatgaccggg
     1321 gtccccgcgg cgacgagcgc cttcatgttg gagtcttcgt cgaccttctt ccccacccgc
     1381 cgggtcatcc cgaaggcgca caagaccgag ttcttccagc gcatcttgcg cgcccgctcg
     1441 aagaactcct tgtccttggg gttgctgccc ggatagccgc cctcgatgta gtggatgccg
     1501 aactcgtcca gcttctcggc gatcgcgacc ttgtcgagca cggacaggga gatctcttcg
     1561 gactgggtcc cgtcgcgcag cgtcgtgtcg tacagatata tggtcttcat tcggcgtccc
     1621 gcccttccga tctcgttttc cccgttcgat gccgcgccgt caggcggggt tccccaatcc
     1681 gaacgctccg tggagcacgc gcacggccaa ctcggtgtac ttctcctcga tgaggacgga
     1741 gatcttgatc tccgaggtgg agatcccgag gatgttgatc ccctccgccg ccagcgtctc
     1801 gaacaccttc agggccacgc cggaatggga gcgcatcgcc atgccgacga tggagacctt
     1861 ggcgatcttg tcgtccccca cgacccgctc cgcccccacg tccttcgccg ccatcgaagt
     1921 gatctgcatc gctttcttgt agtcggtgcg cccgatcgtg aacgtcaggt ccgtgaaccc
     1981 cgtgacggag acgttctgga cgatgacgtc gaccacgatg ttcgcttccg aaagcggctt
     2041 gaagatcttc gccgcgatcc ccgggcggtc ggggaccttg accaccgtga ttttcgcctc
     2101 gctcttgctg tacgccaccc cggacaccac cgccgtttcc atgatctcct cctccgtcgt
     2161 cacgatcgtt ccctggttat cgttgaacga ggagcggacg tggatccgca ccccgtactt
     2221 catcccgaat tccaccgaac gaatctgcag caccttggcg cccaaggagg cgagttcgag
     2281 catctcctcg aaggagatct tgtcgagctt gcgggcgtcg gcgcagatgt tcgggtcggt
     2341 ggtgtacacg ccgtccacgt ccgtgtagat ctcgcagacg tccgccttga gcgcggccgc
     2401 caccgccacg gcgctggtgt cggagccgcc gcgcccgagc gtggtgatgg acccggagtc
     2461 gtcgatcccc tggaaaccgg ccacgacggc gacgtatcca tccttcagcg ctttcgtgat
     2521 cgtctcgccc cggatctggc ggatccgcgc cttcacgtac gcggcgtccg tcagcacggg
     2581 gatctggaag ccgcagaacg acttcgcctt gcaccccatc tcggtgaggg cgatcgccaa
     2641 caggccgatc gtcacctgct cgccggtggc ggcgacgacg tccagctccc gctcgttcgg
     2701 catctccgag atctggttcg ccagcccgag cagccggttc gtctcgccgg acatcgcgga
     2761 gacgaccacg acgacgtcgt tcccctgatc cttcgtccgg gcaactctct tcgccacgtt
     2821 cttgatcttc tcgacggtgc cgaccgaggt cccgccgtac ttctggacaa tgattgccat
     2881 cgcgcctgtc acccccctgg atggaaacgt agggaccggg cccggaagtc gtggaatccc
     2941 gggaggtccg gggcggcgat cgcgatctcc ctgcggtcgt cgcccgaaat agagaattta
     3001 acctgtatac aatccgttgg caacagttcc tcgatctttt ccgcccactc cacaagacag
     3061 acgccgtcgc ccgacaggag ttcgcgcatc ccgatctcgt ccgcttcctg cactcccgca
     3121 aggcgataga ggtcgatgtg cgtgagcggg accggtcccg catgctccgt cacgatcgtg
     3181 aacgtcgggc tgacgatcct gtcgggaggg atccccagcg cctccccgac acctttgcag
     3241 aagagggttt tcccggcgcc aaggtcgccg tacagcgcga cgacgtcgcc gggacgaagc
     3301 gccgcgccca acgcccgcgc gatcgcaagg gtgtcctccg gtgacccgga gtgcaggagg
     3361 aggatcactc ttcgggctgg ggttcgccga gcgtgtgttg cagcgctccc cggatgttgt
     3421 cgatcacttc ggtcgccgtc accggggtca tcgggtggtc gcgcaccagc aggtcggccg
     3481 acatgccgtg caggaagacg cccgcacagg cggcgtcgtt cggcgagaga ccctgggagg
     3541 caagcgccgc gatcatcccg gtcaatgcgt cacccattcc ccccgacgcc atgtacgggt
     3601 tccccgtcgt gttgatgaag atgtcgccct tcggcgtcgc cacgatggtg cgcgctccct
     3661 tgaggatcac cgtgacccgc tcctcttccg ccaggtgccg cgccgagtcg attcgcgtcg
     3721 cctcgatcga ttcgatcgtc tccttcatca accgcgccat ctcccccggg tggggggtca
     3781 ggatgcacgg ggccgtcgcc tcctgcaggg tgaccgcctc ccccgacaag gcgttcagcg
     3841 cgtcggcgtc gagcaggaac ggcaccttga tcctcgagat caactccttc acgaacgcgg
     3901 gcatcgtgtc ggtcatcccc agccccggcc ccacgacgat catgtcggcc ttcgacagga
     3961 cctccatcag cgcggggatc atatccggcg caaaataccc cgtccctccg tccgggatcc
     4021 cgcgggtcat cacctccatc tgcttcgcct cgacgatggg tcgcagcgat tcgggcgtga
     4081 ccacggtgat gagcccggcc cccatccgca gcgccgccga tccggccagg cacggcgcgc
     4141 ccgtcatccc cggcgacccg ccgacgatgt ggacccttcc cgcgtcaccc ttgtggaagt
     4201 cgggcgggcg cactgagagc atgcttttca cgacctgctc gtcgagcgct tcggtcttga
     4261 tctgcgcgtc gaacaccgcc cgcggcggga tcccgatatc gtagatttcc gtctccccgc
     4321 aatggatcga gccgggaagc aaaacgtgtc ccaatttcag cagcccgaag gtccccgtgg
     4381 attccgcgcg gatcgcctcg cccatgatcc ggccggtcgt cgcgtcgatc ccggtcggga
     4441 tgtccacggc gaagacgggt gccatcgaga ggttgatcac ctcgaccacc tcgcgcacga
     4501 tcccggacag cggggacgag aggcccgtcc cgaggatcgc gtccacgcac aggtggacct
     4561 cctcgagata cgtgcgcagg tcttccaccc cttccgtgtc ccggatcacc cgaatctcga
     4621 cgtccatccg ccgaaggatc tcgtgctggg tcctcgcgtc ggtcgacagg tccttctcct
     4681 cgcacaggag gaagacctcg acataggcgc cccggttgtg caggtgccgg gcgatcacca
     4741 tcccgtcgcc gccgttgttc cccttgccgc acaccaccgc cacggacgcc tcctgggggc
     4801 cgcccgcctt ctcctccagg atccggaaga tccggtccgt gcacgaccgc cccgcgttct
     4861 ccatcagcac cagggcgggg atgccgtacg tgtgaatcgt cacccgatcg agttcggcca
     4921 tttgccgtgc cgttacgatt ttcacctatt cctcacccgc cttttcgagg atcacgaacg
     4981 ccaccgcctt ccctccgtca tgcgtgatcg aggcgtgaac ctggctgccg cgccgccact
     5041 tcgcccactt cactgcctgc ccgtgcagat ggacctcggg ctttccgaat cgtccgcgca
     5101 ccgtctcgac gtccttccaa aggatcccct gcgatttccc ggtcccgagc gctttcagca
     5161 tcgcttcctt caccgcgaat cgtcccgcca gccgttccgc cttgttcgcg ccgctcatcg
     5221 cgtaggccgt ttccgcctcg gtgtatacgc ggcgcaggaa ccgttcccca tacaggtcca
     5281 gcagcgtctg gatccgcgcg atatcgacga tatccacgcc gatcccggcg atcatccgga
     5341 ctccctcatg ctccgtggat aaggtcagca atttctttca cggcggcggg cagcccaagg
     5401 aagaccgccc gggcgatgat gctgtgcccg atgttgaact cctcgatctc cgggatgcca
     5461 aggataggga cgatgttgcg gacgtcgagc ccgtggcccg cgaagacctt gagcccgatc
     5521 gaagcgccga atgccgcggc tgaccggatc ttcgcaagct ccgcatcgtg cttccccgct
     5581 ccgaacgcct cgcagaacgt cccggtgtgg atttcgatcg cgtccgctcc cgcctgccgc
     5641 gaagcgcgca cctggggaag ttccgggtcg atgaacatgc tgacgacgat cccggcattt
     5701 ttcagccgct ccgtcgtttg caccatcgac tcccggtacc ccaggacatc cagcccgccc
     5761 tcggtcgtga tctcctcgcg cttctccgga acaagcgtgc aggagtacgg tttcagtccg
     5821 caggcgatcc gcgtgatctc gtcgatcgcc gccatctcga ggttgatccg cgtcgtcacg
     5881 acccgcatca gcacctcgag atcccggtcc tggatgtggc gtcggtcctc ccgcagatgc
     5941 accgtgatcc cgtcggcgcc gtattgctcg gcgatcgacg ccgcgactgc cggttccggg
     6001 acctgtcccc gccgcgcctg gcgcagcgtg gccacgtgat cgatgttgac tccgagccgt
     6061 ttccgcgtca gagtcatcgg gcttcctccc tcgccttctc gcccagggcc ttcacgatcg
     6121 cctcgacctc gtccaggttc tccccttcca ccatgatgcg cagcagcggc tccgtcccgc
     6181 tgtaccggac gacgatccgc ccccggctcc cgagcgcttt ctcgaattcc gcctgggcct
     6241 tttggaaccc cttcatcgac tcgagcggca cccgatgatt catccggatg ttgtgcagca
     6301 tctgcgggaa gacgaccagc cccttcccca gctcggagat cttctttccc gactccacca
     6361 tgacggcgag cagctgcagc gccgccagca cgccgtcgcc cgttgtggcg tgatcgagga
     6421 agatcaggtg ccccgactgc tccccgccga agttgtactt gccggcccgc atcgcctcga
     6481 cgacataccg gtccccgacc ggtgcgcgca ggagcttgat cttccgctcc ttgaggtaga
     6541 gctcgaggcc gatgttgctc atgacggtgg ccaccaccgt gtttttcgac aatcttttct
     6601 tgcgggccat ctccccggcg cagatcgcca tgatccggtc gccgtcctgg acctccccct
     6661 tctggtccac gatgattacc cggtccgcgt ccccgtccag cgagatcccg atgtccgcgc
     6721 gcgcctcccg gaccttagcc gacacaactt cggggtagag ggagccgcac tggtcgttga
     6781 tgttcacccc gttgggagaa acgccgatcg ggatcacctc ggcgcccaac tccgcgaaca
     6841 cctgcggggc gatccgatac gccgcgccgt tggcgcaatc cacaacgatg cgcaacccca
     6901 ccagggagag gttcgtcggg aaggtgctct tcaggtagac gatgtaccgg ccggtagcgt
     6961 cgtcgatccg ctgcgccttt ccgatctgcg gcgatggggg gcgattttcc ttgaggtgct
     7021 cgccgtacat caggctctcg atccggtcct cgacgtcgtc cgggagcttg aaaccgtctc
     7081 cgccgaagaa cttgatcccg ttgtcgggat atgggttgtg ggaggcggag atgacgacgc
     7141 cggcgtcggc ccgcatcgaa tgggtgagga acgcgattcc gggggtgggc atcggcccca
     7201 cgagcaggac atcgcccccc atcgcgcaga tcccggccga cagcgccgtc tcgaacatgt
     7261 agccggacag gcgcgtgtct ttcccgatca cgatcttgtg acggccgttc tttccgcgaa
     7321 acaggtacgt cgccgcctgc cccagcgcaa gcgcggtctc cacggtcatc gggtcggtgt
     7381 tggcgacccc ccgcacgcca tcggtcccga acagccttct caccaaagga atcctcctat
     7441 ttcctgaatt ggatcttcac gcgtacgttc ggattcccga cgattttagc atgcggttcg
     7501 cgcgtttcca cgcgggcgat cgccgagtac gcgccgtctg tccggtccgg cacgacctcc
     7561 tccgtgtaga ccaccggcat ccgcgcgaac tcttccggga gcgcctcgac ctcgaccgac
     7621 gccgggtcga tttctacctt tgcgacccgt ttgcggaccg cagctccccc gccgatcctg
     7681 accgacacct ttagggcccg ccgctccgtc cggtcgagga tcacctcgat cgaggaagga
     7741 tagatccgct gcaccttgat tcccggcggg accttgaccg cccgatcgtc cagggtgaag
     7801 gattggcgcc ccgtgcgcgc gggggtcaga tcgacggcgg cgaagatctc cgaaggacgc
     7861 atccccgaca gcagggacga cggcccggag agacgcacct ccacctgccg ctcggccttg
     7921 ttcgtgaccg tcatcccctt cggcacgttg cggatctcca gcgggatggt gaagctgacc
     7981 aacaccttgt tctcgcccgc gacgaaccac cacgccgcca cggccagagc gagggcgagg
     8041 actttcagcc agagattctg gctcgcgaac cgccggagga tgcggaacag gtcaaccggg
     8101 agcaaggggc gtcacctcca tgcggaacag cagcccgagc gtgtcccgca gctgctgcac
     8161 ggtttcggtg cggttcgccg accggttcga gaagaccgtg acgttccccg tctcttcgga
     8221 gacgaccacc gccaccgcgt ccgtttcgga cgccacgccg aacgccgccc ggtgccgggt
     8281 ccccatcccg cgcgtcacca ccgattcggc cgggatgggg aggatgaccc ccgccgcggc
     8341 gatccggttg cggcggatta ccaccgcccc gtcgtgcacc ggcgagctcg tcgcgaatat
     8401 cgaggcggcc agttcgtggg agaagagcgc gtcgagtttt ttgccgtgct cgacgagctc
     8461 ctcgagcccc gtctcgcgct ccaggaggag gatcgcgccg gtgcgctccc ccgccagcag
     8521 gaaggcgcat tgggcgatct gatcgatcat gtccgggtcc gggacgctcc ccctcccgcc
     8581 gaagaggcgc cactgaccga tcttggcgag cccgcggcgg atctcgctct ggaagacgac
     8641 cacgaggacc acgaggaggt tttcgaggaa gttcccgacc agccactgga aggtgaccat
     8701 cccggccttc ttggcgacca cgtacatcac catcaggatc agcaggccga agagcatctg
     8761 gacggcacgc gtcccccgga tgaacagaag gacccagtag atgatgaagg cgacaagcaa
     8821 gatgtcgagg acgtcgatcg ccccgatgga gaggagtccg cctaccatga tgcctcccca
     8881 cggatcgcgt acgccacttc gaccacttcc cgcatctcct tcacgtcgtg gacgcgcacc
     8941 acgtgggcgc ctcgcagcac cgccgccgcg acggccgccg cggtaccgaa cgaccgttcc
     9001 gccggcggcg cgccggtcag tctccctatg aacgatttgc gcgacgggcc gaagacgatc
     9061 ggccgctcaa ggatccgcag ccgatcaagg tgccggtg
//
LOCUS       DASVJL010000491         3542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1777780, whole genome shotgun sequence.
ACCESSION   DASVJL010000491 DASVJL010000000
VERSION     DASVJL010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777780"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1474
                     /locus_tag="VF357_10160"
     CDS             299..1474
                     /locus_tag="VF357_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX7524557.1"
                     /translation="MDPARKKILTITDRLAPRAARMLREITAYAELPLKEIRTSETLS
                     AFLSERGFRVKRGVAGMETAFRAEFAFGKGRPSVAFLCEMDALPGLGHACGHNIVGVA
                     SACAAAALAKFGNGVFRAGKVIALGTPAEETGYGKARMIEKGVFRDIDAAMMVHPSSR
                     RHVAKGCLALAKLHFTYHGRAAHAAAYPEHGINALDGVLLLFNGVSALRQQLPDTVRV
                     HGIVTEGGCAPNIIPERAKAYFYVRGETLAEMHEAVARVKACAAGAATSSRCRLEVEE
                     GAYTLSPMKVNPVLADAYRGALTLLGFPESGAPTNRNRGSSDIGNVSQVVPTLQPNVP
                     IISGARVEIHTRAFEEATTKPSGIEGMMEGVRALALTGYDLFSDPSLVREAWETFRG"
     gene            complement(1479..>3542)
                     /locus_tag="VF357_10165"
     CDS             complement(1479..>3542)
                     /locus_tag="VF357_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX7524558.1"
                     /translation="GRYPRLAHHANGIPPLRDLAEAVEQAIDANGNVLDRASEELGPL
                     RRQLLKMRSRLQESAEGILSSPRYARYVQETYATVRSGRVVIPFKTSAKGLFQGIVHD
                     SSQSGQTVFFEPEELVYLNNEVKMAELEVEREVARILAELSGRVARKEEELLLALSLL
                     ARIDAIQARSLLADELSAAEPTVTDAGETNLRAALHPLLVLSGRPVVPNDLRLGRPYL
                     CLILTGPNAGGKTVALKMLGLLTLMAMAGLSIPASPDSTVSIFHDLFVAVGDEQSVEG
                     DLSTYSAHIRRLNEILSGADRGSLVLLDEVVSGTDPREGAAIARAFLETLADREVRVV
                     ATTHFEELKGIAFTDTRFENGSMAFDGEHLRPTYRLSLGVPGRSMGIEIARSLGFPEE
                     VLERATGYLSGPGPNLTEVIDRLERERERVRAEAAELAGRRREAEEAARRLDASLAKM
                     KAEESRVVSAARLKMREEIRKAEGELARVTEEMRKDRKIDTVRKASSVIRAWKEKAHA
                     AEEDPAVRTLMSRSAPLAPGEALFPGRKVFVVSLSKEAEVAAPAAAGDREVEVAAGGM
                     MVRVPRDQVRVFPPPGGTRERRADGGSRSVEASPGAVHLQTPGNTLDLRGMYVDDALP
                     EVDAFLDRQSLAQAPHVFLIHGHGTGALKTAVRRHLAASPYLKRSLPAPREQGGDGVT
                     IVLLA"
BASE COUNT          542 a         1245 c         1220 g          535 t
ORIGIN      
        1 caccttcggc ggagggggga tcgggtccgc cgggtcccct tccgcgccga gcagttgctc
       61 gtcatgggcc gtcccgaggg aaccgtgggc gacgaagaac tgggtgacgg cgtcgaccgc
      121 ctcgcggcgg gtctggacgg aaaacgattt ccagcgggtc atggggtacg ggaacgatcc
      181 atggggtatg agaacgatag aagaagggag ggggaaacgc aagtcccttc ggagcgggtg
      241 gtctccctcc ggttgtggta ttatccgcat ctgttccggc aggaaagcgc gagcgcccat
      301 ggatcccgcg agaaaaaaaa tcctgacgat cacggaccgg ctggcccccc gggccgcccg
      361 gatgctgcgc gaaatcacgg cgtacgccga actccccctg aaagagattc gcacttcgga
      421 aaccctctcc gcgttcctct cggagcgggg gtttcgcgtg aagcgcggcg tggccgggat
      481 ggagacggcc ttccgggcgg agttcgcctt cggaaaaggg cggccctccg tcgcgttcct
      541 gtgcgagatg gacgcgctgc ccgggctggg gcacgcgtgc gggcacaaca tcgtgggggt
      601 ggcgtcggcg tgcgccgccg cggcgctggc gaagttcggg aacggcgtct tccgggcggg
      661 gaaggtgatc gccctcggga cccccgccga ggagacgggg tacggcaagg cccggatgat
      721 cgagaagggg gtcttccggg acatcgacgc ggcgatgatg gtccacccgt cgtcccgccg
      781 ccacgtggcg aagggatgtc tcgcgctggc caagctgcac ttcacgtacc acggccgggc
      841 ggcgcatgcg gcggcgtacc cggagcacgg gatcaacgcc ctcgacggcg tccttctcct
      901 cttcaacggc gtctccgcgt tgcgccagca gcttccggac accgtgcgcg ttcacggcat
      961 cgtcaccgaa ggggggtgcg cccccaatat catccccgag cgggcgaagg cgtacttcta
     1021 cgtacggggc gagacgctcg ccgagatgca cgaagcggtc gcgcgcgtga aggcgtgcgc
     1081 cgccggcgcg gcgacgtcct cccgctgccg gctcgaagtg gaggaggggg cgtataccct
     1141 ttctccgatg aaggtgaatc ccgtcctggc cgatgcctac cggggagcgc tgacgctgct
     1201 cgggtttccg gagagcggcg cgccgacgaa ccggaaccgc ggctcctccg atatcgggaa
     1261 cgtctcccag gtcgtcccaa cgctgcagcc gaacgtcccc atcatctcgg gcgcccgcgt
     1321 cgaaatacac acccgcgcct tcgaggaggc gacgacgaaa ccgtccggca tcgaagggat
     1381 gatggagggg gtccgggcgc ttgccctcac cgggtacgac ctcttctccg acccgtccct
     1441 cgtgcgcgag gcatgggaaa cgttccgcgg ataaggaatc aggcgagaag gacgatcgtg
     1501 acgccgtcgc cgccctgttc gcggggggcg gggagggacc gcttcaggta gggggacgcc
     1561 gcgaggtggc gacggaccgc cgtcttcaac gcgccggtgc cgtgcccgtg gatcaggaaa
     1621 acgtggggcg cctgggccag cgactgccgg tcgaggaagg cgtcgacctc ggggagggcg
     1681 tcgtccacgt acatccctcg caggtcgagg gtgttcccgg gcgtctggag gtgcaccgcc
     1741 cccggcgacg cctccaccga ccgcgacccg ccgtccgccc ggcgctcccg cgtccctccc
     1801 ggcggcggaa agacgcggac ctggtcgcgc gggacacgga ccatcatccc gcccgcggcc
     1861 acctccacct cgcggtctcc ggccgccgcc ggcgcggcca cttccgcctc cttcgacagc
     1921 gacacgacga acaccttcct gccgggaaag agcgcctccc cgggtgcaag gggagcggac
     1981 cggctcatca gcgtccggac ggcggggtcc tcctccgccg cgtgcgcctt ctccttccat
     2041 gcgcggatca ccgaggaggc cttccgcacg gtgtcgatct tccggtcctt ccgcatctcc
     2101 tccgtgaccc gggcgagttc cccttccgcc ttccggatct cctcccgcat cttcaggcgc
     2161 gccgcggaga ccacccgaga ctcctcggcc ttcatcttcg ccagtgaagc gtcgagccgc
     2221 cgcgccgctt cctccgcctc cctgcgccgt cccgcaagct ccgccgcctc ggcacgcacc
     2281 cgttcgcgct cccgctccag gcggtcgatc acctccgtca ggttcgggcc gggcccggag
     2341 aggtaccccg tcgcccgctc gagaacctcc tccggaaagc cgagggaccg cgcgatctcg
     2401 atccccatgg aacggcccgg gactcccagc gacagccggt acgtcggccg gaggtgttcc
     2461 ccgtcgaacg ccatcgagcc gttctcgaac cgggtatccg tgaaggcgat ccccttcagt
     2521 tcctcgaagt gcgtcgtcgc caccacgcgc acctcccggt ccgccaacgt ttccaggaag
     2581 gcgcgggcga tcgccgcccc ttcgcgcgga tccgtccccg agacgacctc gtcgagcagc
     2641 accagcgaac cgcggtccgc gcccgagaga atctcattca gccgccggat gtgggcggag
     2701 taggtggaca ggtccccctc cacgctttgc tcatccccca ccgcgacgaa caggtcgtgg
     2761 aagatagaca cggtcgaatc cggggaggcg gggatggaca gccccgccat cgccatcagc
     2821 gtgagcagcc ccagcatttt cagggccacg gtcttcccac ccgcgttggg gccggtgagg
     2881 atcaggcaca ggtacgggcg gcccaggcgc agatcgttcg ggaccaccgg gcgtccgctc
     2941 agaaccagca gcgggtggag ggcggcacgc aggttcgtct ccccggcgtc ggttaccgtg
     3001 ggctcggcgg cggaaagctc gtccgccagc agggagcgcg cctggatcgc gtcgatccgg
     3061 gcgaggaggg agagggcaag gagcagttcc tcctcctttc gggcgacccg accggaaagc
     3121 tcggcgagga tccgcgccac ctcccgctcc acctcgagtt cggccatctt cacctcgttg
     3181 ttgaggtaga cgagttcctc cggctcgaag aagaccgtct ggccgctctg ggatgagtcg
     3241 tggacgatcc cctggaagag ccccttcgcg ctcgtcttga aggggatcac gacgcggccc
     3301 gaccggacgg tggcgtatgt ttcctggacg tagcgggcgt agcgcgggga ggagaggatc
     3361 ccctccgccg actcctgcag gcgggagcgc atcttcagga gctggcgccg caagggcccc
     3421 agttcctccg acgcccggtc gagcacgttc ccgttggcgt cgatcgcctg ctcgaccgcc
     3481 tcggcgaggt cgcgcagcgg cgggatcccg ttcgcgtggt gggccagccg cgggtacctt
     3541 cc
//
LOCUS       DASVJL010000492         3766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1216688, whole genome shotgun sequence.
ACCESSION   DASVJL010000492 DASVJL010000000
VERSION     DASVJL010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216688"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1649
                     /locus_tag="VF357_10170"
     CDS             <1..1649
                     /locus_tag="VF357_10170"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7524559.1"
                     /translation="GALSLVYPGLGHLYSGRIAYGVFWAALLPLSLGLVLTVGSGITF
                     GHGFLLVEAGVIWWMAYVDARRGPREAAAPCESACPARIRVPDYIALVREGRPLEALA
                     LVHDKLPFAAFCGRACPHPCEQKCVRNECGAPISIMAIKRYAADLGYAAGIPPSSGGT
                     GRVRRPKVAVVGAGASGLSAADTLARLGGLVTVFDPYGEPGGMMRYGAAEFRFPGDAL
                     LADVTRVLARGVRFRGGITFGKDVTFSSLAAEGFDAVLIAVGAREALRLPAAGGEDQG
                     FHDALSFLVRVREHRYPRLRGRVVVIGGGNVAVDAARCALRMGASGVTIACVESREAM
                     PAFTWEVEAAVSEGAKFLPGTAVNRFLLKDGRVAAFEALKVERVDLDPNGEVVPRTVP
                     GTEFEVPADTVVMAIGTRPDLSFLPAGVSWKPTDPARNVFRLQFPAGEPRIAGYMCGD
                     CVRGPGTVVEASASGRETALNIFEDLAVEEVGRARYKDNYRRRPESHVPDRPGWRVRR
                     RAERLSPEAAIGTFDEVEMRLTDRCAREEAERCARCNLSL"
     gene            1678..2028
                     /locus_tag="VF357_10175"
     CDS             1678..2028
                     /locus_tag="VF357_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX7524560.1"
                     /translation="MKKFAMLMAVVMVAVFSASVIVAADAPEKATINEVQKSKPEVVF
                     PHKAHADRIKNCAECHHKDAAGKEQKCFACHKAEKKDKAVSLKDAMHTKCKDCHAKDA
                     SKKAPTKCDGCHKK"
     gene            2201..3532
                     /locus_tag="VF357_10180"
     CDS             2201..3532
                     /locus_tag="VF357_10180"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX7524561.1"
                     /translation="MDRVWDFFISVKLAIITLIVLAATSILGTIIEQNQPAEKYHQIY
                     EDWAFALMDRVNLFDMYHSMWFLLMLVLFTVNLSCCTIDRFPKMLRVVRNPRTKLDGS
                     LEKSLSLSDRWKRKGTLSEWAGKYAEALSGSFAKPNVTEDGGEVHLYAETGVASRFGV
                     YVTHLSIVIIFIGAIIGNVVGFKGYVNIPEGEAVTQVPVRGGTRMQDLGFTVRCNAFS
                     LETYPSGQPKAYKSDLSVIDGGREVIRKTIVVNDPLQYKGIWFYQSSYGQAGGATAQV
                     AVARKDGTPVSMLALTANEPVPIDGYGIMRGVNYEQNFEGQGPALQVVVEKPGQPAAS
                     IWLVQGRSDLDRQRADSLVFSFRGLSSKMFTGLQVAKDPGVNIVWLGCTLMVIGLIVA
                     FFLSHQRVWVRLAQGTDGRVEVVLAGSASRNRLAFEKRFEKLQTGVKATGQ"
     gene            3570..>3766
                     /locus_tag="VF357_10185"
     CDS             3570..>3766
                     /locus_tag="VF357_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524562.1"
                     /translation="MNNVILFNLTTILFGVASALYLGALYAKNEKISAFGTWVCVLAA
                     VVSTAALGLRWYESYQMGIGR"
BASE COUNT          653 a         1160 c         1295 g          658 t
ORIGIN      
        1 caggggccct ttcgctcgtc tacccggggc tcgggcatct ctactcgggc cggatcgcct
       61 acggggtctt ctgggcggcc ctccttcccc tgagcctggg actcgtcctc accgtgggat
      121 ccgggatcac cttcggccac ggcttcctgc tggtcgaggc gggcgtgatc tggtggatgg
      181 cgtatgtcga cgcgcggcgc ggcccccgcg aagcggcggc tccgtgcgag agcgcctgcc
      241 cggcccgcat ccgcgttccg gactacatcg cccttgtgcg cgaggggcgg ccgctcgagg
      301 cgctggcgct ggtccacgat aaacttcctt tcgcggcgtt ctgcggacgc gcctgtccac
      361 atccgtgcga acagaagtgc gtgcggaacg agtgcggagc gccgatttcc atcatggcga
      421 tcaaacggta cgctgcggat ctcgggtacg ctgcggggat acccccgtcc tccgggggaa
      481 ccggacgggt ccgaaggcct aaagtggcgg tcgtcggcgc cggcgcgtcc ggattgtcgg
      541 ccgccgacac gctcgcgcgg ctcgggggcc tcgtcacggt gttcgatccg tacggggaac
      601 ccggcgggat gatgcggtat ggagcggcgg agttccggtt tccgggggac gcgctcctcg
      661 cggacgtgac gcgggtcctt gcccgggggg tgcgcttccg gggcgggatc accttcggca
      721 aggacgtgac gttctcttcc ctcgcggcgg aggggttcga cgccgtcctg atcgccgtgg
      781 gagcccgcga agcgctgcgg ctgcctgccg cgggagggga ggatcaggga ttccacgacg
      841 cgctttcgtt cctcgtgcgc gttcgggaac accggtatcc tcggctccgc ggacgggtgg
      901 tcgtgatcgg gggggggaac gtcgcggtcg acgcggcccg gtgcgctcta cggatggggg
      961 cgtcgggagt gacgatcgcc tgcgtcgagt ccagggaggc gatgccggcg ttcacgtggg
     1021 aggtcgaggc ggccgtttcg gagggggcga agttccttcc gggcacggcg gtgaaccgtt
     1081 tcctcctgaa ggatggcagg gtggccgcgt tcgaggcact gaaagtggag cgggtcgacc
     1141 tggacccgaa cggcgaggtc gtcccgcgca ccgtccccgg caccgaattc gaggtcccgg
     1201 ccgacaccgt cgtgatggcg atcggaaccc ggcccgacct ctcgttcctg cctgccggag
     1261 tctcctggaa gcccacggat ccggcgcgca atgttttccg cttgcaattt ccggcggggg
     1321 aacctagaat cgccggctat atgtgcggtg attgcgtccg cggccccggc accgtggtcg
     1381 aggcgtccgc ctccgggcgg gaaaccgcct tgaatatctt cgaggatctc gccgtcgaag
     1441 aggtcggaag ggcgcgatac aaggacaact accgcaggcg ccccgaatcc catgtgcccg
     1501 accgccccgg atggcgtgtt cgacgcaggg cggagaggct ctctcccgag gcggcgatcg
     1561 gaacgtttga tgaagtcgaa atgaggctca cggatcggtg cgcgcgcgag gaagcggaac
     1621 gatgcgcacg gtgcaatctc tcgctgtaga ccacaaaccg aaagggggtg agcagggatg
     1681 aagaaattcg cgatgctgat ggcagttgtc atggtggcgg tgttttcggc cagcgtgatc
     1741 gtcgcggcgg acgctcctga gaaggcgacg atcaatgagg tgcagaagtc gaagccggag
     1801 gtcgtgttcc cgcataaggc gcacgcggac cggatcaaga attgcgcgga gtgccaccac
     1861 aaggacgctg ccggcaagga gcagaagtgc tttgcctgcc acaaggccga gaagaaggac
     1921 aaagcggtct ctttgaagga cgccatgcac acgaagtgca aggactgcca tgcgaaggac
     1981 gcgtcgaaga aggctccgac gaagtgtgac ggctgccaca agaagtaggc gacccgcggt
     2041 tccgcgagcg ctgaaagagg gcggggagtt tccccgccct ctttcattcg gggcggattt
     2101 caattccgtt tgacggccga cacccgatct ggtatgcagt atacattccg aataaccgag
     2161 ggtgacccgc atggttgacg agaaacgtac tccatccccg atggatcggg tatgggactt
     2221 cttcatctcc gtcaagctgg ccatcatcac cctgatcgtc cttgccgcca cgtcgatcct
     2281 cggcacgatc atcgagcaga accagcccgc agagaagtac caccagatct acgaagactg
     2341 ggcgttcgcc ctcatggacc gggtcaacct gttcgatatg taccactcca tgtggttcct
     2401 attgatgctc gtcctgttca ccgtgaacct gtcctgctgc accatcgacc gcttcccgaa
     2461 gatgctgagg gtggtgcgga acccgaggac gaagcttgac ggaagcctcg aaaaatcgct
     2521 gtcgctctcg gaccggtgga agcggaaggg gaccctttcg gagtgggccg gaaaatacgc
     2581 ggaagcgctc tccggttcct tcgcgaagcc gaacgtcacc gaggacgggg gcgaggtgca
     2641 cctgtatgcc gaaaccggtg tggcctcccg gttcggcgtc tatgtgaccc acctctccat
     2701 cgtgatcatc ttcatcgggg cgatcatcgg caacgtcgtc ggcttcaagg ggtacgtgaa
     2761 catccccgaa ggggaggcgg tcacgcaggt ccccgtgcgc gggggaaccc ggatgcagga
     2821 cctcgggttc acggtccggt gcaacgcgtt cagcctcgag acatacccga gcggccagcc
     2881 caaggcatac aaatccgacc tcagcgtcat cgacggaggt cgggaagtga tccggaagac
     2941 gatcgtggtg aacgacccgc tgcagtacaa ggggatctgg ttctaccagt cgagctacgg
     3001 acaggccggg ggcgcgacgg cccaggtggc ggtggcccgc aaagacggga ccccggtgag
     3061 tatgctcgcc ctcacggcca acgagccggt tccgatcgac ggctacggga tcatgcgcgg
     3121 ggtgaattac gagcagaact tcgaggggca aggcccggcc ctccaggtgg tcgtggagaa
     3181 gcccgggcag ccggccgcct cgatctggct cgtccaggga cggtccgacc tcgaccggca
     3241 acgcgccgat tcactggtct tctccttccg cgggctgagc tcgaagatgt tcaccgggct
     3301 tcaggtcgcg aaggaccccg gggtaaacat cgtctggctg gggtgcaccc tgatggtgat
     3361 cggactcatc gtggcgttct tcctctcgca tcagcgggtc tgggtccggc tcgctcaggg
     3421 gacggacggc cgtgtcgagg tcgtcctcgc cggctctgca agccgcaacc ggcttgcctt
     3481 cgagaagaga ttcgagaaac tacagaccgg cgtgaaggcc acagggcaat gaccgcccgg
     3541 ccgcaaaccg ccgcaggagc cccctcgaaa tgaacaacgt catcctgttc aacctcacca
     3601 cgatcctgtt cggcgtggcg tccgccctgt acctcggcgc tctctacgcg aagaacgaaa
     3661 agatctcggc gttcggcacc tgggtctgcg tcctcgcggc cgtcgtttcc accgcggcgc
     3721 tcgggctgcg ttggtacgag tcgtaccaga tggggatcgg tcgcat
//
LOCUS       DASVJL010000493         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_88161, whole genome shotgun sequence.
ACCESSION   DASVJL010000493 DASVJL010000000
VERSION     DASVJL010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88161"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VF357_10190"
     CDS             complement(<1..989)
                     /locus_tag="VF357_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7524563.1"
                     /translation="MRIDCVSGDIRAVKADMLVVNLFEGAKRPGGAAAAVDAAIGGAI
                     AAAVRGGDFTGKLGETLSLRPSRGLFSPRVLVVGLGKKEKFTSDHARQAVLPVLKAAK
                     RLKLATVASVVHGAGAGGVDPGIAARFCALGAALSAFEFDRYKEEKAHRIARFLFVER
                     DAKAFPSVRAGVSWGARVGEAINWGRFLVATPPAELRPDDLARAARGVGRGIGAVAAR
                     KVRVRVLGLPELSALKAGGILAVAKGSPASPRLVVAEYRGGIPGSPWTALVGKGVTFD
                     TGGISLKKWEGMEKMKYDMAGGAGMLATLRAAAALGIRRNLVAVVPCVENMPSG"
     gene            complement(1208..1912)
                     /locus_tag="VF357_10195"
     CDS             complement(1208..1912)
                     /locus_tag="VF357_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7524564.1"
                     /translation="MLKVSGIDVFYGDLQVLWDVSFEVREKEILVLVGANGAGKSTTL
                     KTISGLLKPRKGSIEFDGVRLDQLPPDRVIGRGVVHVPEARRLFREMSVEENLIMGSL
                     SPEAKKKRYETMDWVYQLFPRMKERRKQSAGTLSGGEQQMCAIGRGLMALPKVLMFDE
                     PSLGLSPILVQEVFEIAKRINKEGVTVLLVEQNVRQTLAMCDRAYVLENGRVVLEGTG
                     KILMDDPHVKEAYLGI"
BASE COUNT          336 a          741 c          650 g          315 t
ORIGIN      
        1 ccggagggca tgttctccac gcaggggacg acggcgacga ggttccgccg gatcccaagg
       61 gcggcggcgg cccgaagggt cgccagcatc cccgcgcccc ccgccatgtc gtacttcatc
      121 ttctccatcc cctcccactt cttgagggag atgccgccgg tgtcgaaggt gacccccttc
      181 ccgacgaggg cggtccaagg ggatccgggg atcccgccgc gatactccgc gacgaccagg
      241 cgcggcgacg cggggctgcc cttcgccacg gcgaggatcc cgccggcctt cagggcggag
      301 agttcgggga ggccgaggac gcggacgcgc accttgcgcg cagcgacggc gccgatcccg
      361 cggcccaccc cccgcgcggc gcgggccagg tcgtcgggcc gtagctccgc cggcggggtc
      421 gccacgagga agcggcccca gttgatcgcc tcgccgaccc gggcccccca ggacactccg
      481 gcgcgcacgg aggggaacgc cttcgcatcc cgctcgacaa aaagaaaccg ggcgatgcgg
      541 tgcgccttct cctccttgta ccggtcgaac tcgaaggccg acagggcggc gccgagggcg
      601 cagaagcgcg cggcgatccc gggatcgacc ccgcccgcgc ccgctccgtg gacgacggac
      661 gccacagtgg caagcttgag gcgcttcgcc gccttcagga ccggcaggac ggcttggcgc
      721 gcgtgatccg atgtgaattt ttccttcttc ccgaggccga ccaccaggac ccgcggggag
      781 aagagacccc gggagggacg cagggataag gtttccccga gcttccccgt gaagtcgccg
      841 ccgcggaccg ccgccgcgat cgcgcccccg atcgccgcat cgaccgcggc ggccgccccg
      901 ccgggccgct tcgccccctc gaacaggttc accaccagca tgtccgcctt gaccgcgcgg
      961 atgtctccgg aaacgcaatc tatacgcata tgcctacccc cgatcttcgc actcgacaga
     1021 ctggtcgccc ctgcgatctg agggccccat cccggcaata cggtgaagca tcgagagcgt
     1081 tgatgcgccg tgcccgcaag gcgcgcgact gaggcgtact acctgtacac cgcagggagc
     1141 gcaacgccgc gggcacggat gcagcgacgc tcgaatgcca gcggattgac gggatgggat
     1201 gggaccctca gatcccaagg tacgcctctt tgacgtgggg gtcgtccatg aggatcttgc
     1261 cggtcccctc cagaacaacc cgcccgttct cgagcacgta tgcgcggtcg cacatggcga
     1321 gggtctgccg gacgttctgc tccaccagaa ggaccgtgac cccctccttg ttgatccgct
     1381 tcgcgatctc gaagacctcc tgcacgagga tgggggaaag ccccagcgac ggctcgtcga
     1441 acatgaggac cttcggcagc gccatcagcc cccgcccgat cgcgcacatc tgctgctcac
     1501 ctccgctcaa cgtcccggcg gactgtttcc gccgctcctt catccgcggg aagagctggt
     1561 acacccagtc catcgtctcg taccgcttct tcttcgcctc gggggaaagg gagcccatga
     1621 tgaggttctc ctcgacggac atctcccgga acagacgacg cgcttcgggg acgtggacaa
     1681 ccccccggcc gatcacgcgg tccggcggaa gctggtcgag gcggacgccg tcgaactcga
     1741 tcgatccctt ccggggcttg aggaggcccg agatcgtctt gagggtggtc gattttcccg
     1801 caccgttggc ccccaccagg accaggatct ccttctcgcg cacctcgaag gagacgtccc
     1861 agaggacctg gaggtccccg tagaagacgt cgatccccga caccttaagc atagtccgtc
     1921 cccaggtagg cctcgatcac cgcctggttc gccgccactt ccttcggcgt tccctcggcg
     1981 atcgtctgcc cgaagttgat cgcgtggatc cggtcggaga tggtcatgat cacgtgcatg
     2041 at
//
LOCUS       DASVJL010000494         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_276831, whole genome shotgun sequence.
ACCESSION   DASVJL010000494 DASVJL010000000
VERSION     DASVJL010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276831"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VF357_10200"
     CDS             <1..267
                     /locus_tag="VF357_10200"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7524565.1"
                     /translation="LSGLGYPNGLSGSAINLFGRICAIADCYDAMTTRRLYQRAYTPY
                     EALFQIMKEVGNYDRDVLESFIRMLGKVGVPEKSHAKAISLLPG"
     gene            455..1291
                     /locus_tag="VF357_10205"
     CDS             455..1291
                     /locus_tag="VF357_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX7524566.1"
                     /translation="MSLVINTNISSMNAQRNLATNSEKLATSVQRLSSGLRINSAKDD
                     AAGLAISEKIRSQVRSISVAIRNSQDGISLAQTTEGGLQEIGNIVGRMRELSTQSANG
                     TLGQTERNSLDAEYQQLKTEIDRISNATEFNGTKLLNGSQSASGVTLQVGFQNVAAND
                     RITFFSGIGATSVSTLSLMADISTVASAQSNLDMTSSAIGTVASSRGTLGAVMSRLES
                     TISNLRVTSENLTAADSAIRDADFAYESSQFTKNQIMVQAANAMVAQANTLPQSALQL
                     LK"
     gene            1355..1735
                     /locus_tag="VF357_10210"
     CDS             1355..1735
                     /locus_tag="VF357_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HEX7524567.1"
                     /translation="MDVKVDMALPISPLAFTGPSGSAASGKHAPPPPSAGKGAGTQSP
                     DIAEVQKVVREALNAEPLPDRELRIEFESDLHRIVVKVLDKENGKVIRQIPMPESLAI
                     AKDIRAQLSRMAREQRAISVDQEV"
     gene            1736..>2800
                     /gene="fliD"
                     /locus_tag="VF357_10215"
     CDS             1736..>2800
                     /gene="fliD"
                     /locus_tag="VF357_10215"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEX7524568.1"
                     /translation="MSTFSVSGLTSGIDYNTMIDQIMKLERQPETRMQTQQAAYNKTI
                     SVYGDLSSKLAALKTAAEGLKTTTSFYARTASSSDATVFDATAASSAAAGNYSITVTA
                     LAQAHRVASSPVAAESSTVSVASGNFSFHVGAAGTMTTVAVDTTTTLANLRDAINAQN
                     GDAEASIIYDGTGYRLALTSKTSGAVNAITVTENVTSLGLPTGPVAGGTQLQAAQDAA
                     FSIDTLSMTRSTNTFSDAIGGVTITMKKGGTSTLSVTTDTAAIQKKIEGFVSAYNDIV
                     SLVSTNATYDTKTNIGGPLSGESTARDVMSRLQEILGSRVAGLPDGLRALSQIGIKTG
                     NDGTLSIDSAVLSDKIAKNLA"
BASE COUNT          629 a          913 c          841 g          417 t
ORIGIN      
        1 ctctccggcc tcgggtaccc taatggactg tccgggagcg ccatcaacct gttcgggcgg
       61 atctgcgcga tcgccgactg ctacgacgcg atgacgaccc gccgcctgta ccaacgggcc
      121 tacacccctt acgaggccct ctttcagatc atgaaggaag tcggaaacta cgaccgggac
      181 gtcctcgaat ccttcatccg gatgctcggg aaggtcgggg tgccggaaaa atcccacgcc
      241 aaggcgattt cccttctccc gggctaaaga attccgggga acggccgatt aatataccag
      301 aggccgaacg aggcaaagct ggagaggaga ggaaacggaa gaacgccggg caggaacgcc
      361 ggcaatacag ccgaagcgct cgcgggttga agaaaccacg gtttcaaaaa aaatcgggca
      421 aggatgccca aactattcca aggaggaata aaaaatgtct ctcgtaatca acacgaacat
      481 ttcctcaatg aatgcccaga gaaacttggc taccaacagc gaaaagctcg ccacgtcggt
      541 ccagaggctt tcgtccggac tccgcatcaa cagcgcgaag gacgacgccg ccggtctggc
      601 catttccgaa aagatccggt cacaggtccg cagcatctcc gtcgcgatcc gtaactccca
      661 ggacggcatc tccctcgctc agacgacgga aggcggcctc caggagatcg ggaacatcgt
      721 cggccggatg cgcgagcttt cgacgcaatc cgccaacggc accctcgggc agacggaacg
      781 taactcgctc gacgccgaat accagcagct gaaaaccgaa atcgaccgga tctcgaacgc
      841 caccgagttc aacggaacca agcttctgaa cggctcccag agcgccagcg gcgtcacgct
      901 gcaggtcggg ttccagaacg tcgccgccaa cgaccggatc acgttcttca gtgggatcgg
      961 tgcaacgagc gtgtcaaccc ttagcctgat ggcggacatc agcacggtcg cgagcgccca
     1021 gtcgaacctc gacatgactt cgagcgcgat cggcacggtc gcttccagcc gcggcacgct
     1081 cggcgcggtc atgagccggc tcgagtcgac gatctcgaac ctgcgggtca cctccgagaa
     1141 cctcaccgcc gcggattcgg cgatccggga cgcggacttc gcatacgagt cgtcccagtt
     1201 tacgaagaac cagatcatgg tccaggcagc gaacgcgatg gtcgcacagg ccaacacgct
     1261 gccgcagtcg gctctccaac tcctgaaata ggagcgttga aaacctccgg ggaggggggg
     1321 accccccctc cccgggcaat tccgggtgaa cgacatggac gtaaaagtcg acatggccct
     1381 tccgatctcc cccctggcgt ttacggggcc gtccggttcc gccgcgtcgg gcaagcacgc
     1441 tccccctccg ccctccgccg ggaagggagc cgggacccaa tctcccgaca tcgccgaagt
     1501 ccagaaggtc gtgcgcgaag cgctgaacgc cgaacccctc ccggaccggg agttgcggat
     1561 cgagttcgaa agcgacctcc acaggatcgt ggtcaaggtc ctggacaagg agaacggcaa
     1621 ggtgatccga cagatcccga tgcccgaatc gcttgcgatc gcgaaggaca tccgggcgca
     1681 actcagcaga atggcccgcg aacagcgcgc gatctccgtg gaccaggagg tttaagtgtc
     1741 gacattctcg gttagcggcc tgacgtcggg gatcgactac aacaccatga tcgaccagat
     1801 catgaagctg gagcggcagc cggaaacccg gatgcagacc cagcaggcgg cttacaacaa
     1861 gacgatcagc gtttacggag acctctcctc gaagctggcc gcgctgaaga ccgccgcgga
     1921 ggggttgaaa accaccacga gcttctacgc caggacggcg tcgtcgagcg acgcgaccgt
     1981 cttcgacgca acggcggcga gttccgccgc ggcggggaac tattccatca ccgtgaccgc
     2041 gcttgcgcag gcccacaggg tcgcctcgtc gccggtggcg gccgaatcga gcacggtaag
     2101 cgtcgcgagc ggcaacttca gcttccatgt cggcgccgcg ggcaccatga cgaccgtagc
     2161 ggtcgatacg acgacgacgc tggcgaacct gagggacgcg atcaatgcac agaacgggga
     2221 cgcggaggcc tccatcatct acgacgggac cggctaccgc ctcgccctga cgagcaagac
     2281 ctccggcgcg gtcaacgcca tcaccgtgac cgagaacgtc acctccctcg gtcttccgac
     2341 cggtcccgtc gcgggcggga cacaactcca ggcggctcag gacgccgcgt tcagcatcga
     2401 cacgttatcg atgacccgga gcaccaacac gttctcggat gcgatcgggg gcgtcacgat
     2461 cacgatgaag aaggggggga ccagcaccct ctccgtgacg accgacacgg cggccatcca
     2521 gaagaagatc gaagggttcg tctccgccta caatgacatc gtatccctcg tctccaccaa
     2581 cgccacttac gacacgaaga cgaatatagg cggaccgttg tcgggcgagt caacggcccg
     2641 ggacgtcatg agccggctgc aggagatcct cgggagtcgc gtcgcggggc ttccggatgg
     2701 gttgcgggct ctctcgcaga tcggtatcaa gaccggcaat gacgggacac tctccatcga
     2761 cagcgccgtg ctcagcgaca agatcgcgaa gaacctggcc
//
LOCUS       DASVJL010000495         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1402361, whole genome shotgun sequence.
ACCESSION   DASVJL010000495 DASVJL010000000
VERSION     DASVJL010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402361"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /gene="cdhC"
                     /locus_tag="VF357_10220"
     CDS             <1..1299
                     /gene="cdhC"
                     /locus_tag="VF357_10220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_function: GO:0043884 - CO-methylating acetyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HEX7524569.1"
                     /translation="GDAMVQAGMEARGIKVKMVKVPIPIAFGPAFEGERIRKADTHVE
                     FGGGRSVAYELVRSASPEEVHDREIVVDGPDLRGLPKGSVLPLGIVVRVAGARMQHDF
                     ESVLERRIHRIVNYGEGTMHVAQRDTTWIRVSTEAVEKGFSLADLGLMLYAKLHADFE
                     NIVDKVSVSITTRLEEVQAGLAGARAAYAERDARARTLTDDAVSEFYSCTLCQSFAPN
                     HVCIVSPERLGLCGAINWLDGKAGFEITPTGPNQPVRKGAVRDAVKGSFDGVNAFVRE
                     NSRNTVTEMNLYSIMESPMTSCGCFECVLAVIPEANGVMAVNREFTGLETPCGMGFST
                     LAGSVGGGVQTPGFMGVGKHYLLSRKFLGAEGGLARVVWMTREMKEYLGPLLRERAAE
                     IGMPDLVDQIADESIAVTPDALVEHLVKVGHPALKLPPLL"
     gene            1344..>1991
                     /locus_tag="VF357_10225"
     CDS             1344..>1991
                     /locus_tag="VF357_10225"
                     /inference="COORDINATES: protein motif:HMM:NF015556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524570.1"
                     /translation="MTLPAGPGGTPVTVGGQNALPLLPEGEHPNAPAVGMEVPTGPLE
                     LPPALAATIGPFAGDPVAMARKCAGDWGARFLFLSLAGCHPDAGGQSVSFGAAVVREV
                     LSATPAPVIVGGCGDEEVDARLFPAVAEAAGRPVFLSYAEEKNYRLVAGAAMAYGHGV
                     VSWSPIDINMAKQMNLLLADLGFPPDRVLIDPLTGGLGYGMEYTFTIVERIRLAGL"
BASE COUNT          306 a          665 c          701 g          319 t
ORIGIN      
        1 ggcgacgcaa tggttcaagc ggggatggag gcgcggggca tcaaggtgaa gatggtcaag
       61 gtgccgatcc ccatcgcctt cggtcccgcc ttcgaagggg agcggatccg gaaggccgat
      121 acgcacgtcg agttcggcgg cgggcgctcc gtggcatatg aactcgtccg cagcgcctcc
      181 ccggaagagg tgcacgaccg ggagatcgtc gtggacggcc ccgatctcag ggggctgccg
      241 aaagggagcg tcctcccgct ggggatcgtc gtccgggtcg ccggtgcgcg gatgcagcac
      301 gacttcgaat cggtcctcga gcgccgcatc caccggatcg tcaattacgg cgaggggacg
      361 atgcacgtcg cccaacggga caccacctgg atccgcgtct cgacggaggc ggtcgagaag
      421 ggattctccc ttgcggacct cggcctcatg ctctacgcca agctgcacgc ggacttcgag
      481 aacatcgtgg acaaggtgtc ggtttcgatc acgacccgac tggaggaggt gcaggcgggg
      541 ctggccgggg cgcgggccgc ctacgccgag cgggacgcgc gggcgcgcac gctgaccgac
      601 gacgccgtct ccgagttcta ctcctgcacc ctgtgccagt cgttcgcccc caaccacgtc
      661 tgcatcgtct cccccgagcg cctcgggctg tgcggagcga tcaactggct cgacgggaag
      721 gcggggttcg agatcacccc gaccggcccc aaccagccgg tgcggaaggg agcggtccgg
      781 gacgccgtga agggatcgtt cgacggggtg aacgcgttcg tccgggagaa ttcgcgcaat
      841 accgtgaccg agatgaacct ctactccatc atggagtcgc cgatgacctc gtgcggctgc
      901 ttcgaatgcg tcctcgccgt cattcccgag gcgaacggcg tgatggcggt caaccgcgaa
      961 ttcaccggac tggaaacgcc gtgcgggatg ggattctcca cccttgccgg atccgtcggg
     1021 ggcggcgtac agaccccggg cttcatgggc gtggggaaac attacctgct gagccggaaa
     1081 ttcctcgggg ccgagggggg ccttgcgcgc gtcgtctgga tgacccggga gatgaaggag
     1141 tacctcgggc cgctcctgcg ggagcgggcg gcggagatcg ggatgcccga ccttgtcgat
     1201 cagatcgcgg acgaatcgat cgcggtgacg cccgacgcgc ttgtcgaaca cctggtgaag
     1261 gtgggacacc ccgccctcaa gctccccccc ctgctgtgag cgtggcgctt cccattccgg
     1321 gctttcccgg aaggatcctc gcggtgacgc ttcccgccgg gccgggggga actcccgtca
     1381 ccgtgggagg gcagaacgcc ttgccgctcc tgccggaggg ggaacacccc aacgcgccgg
     1441 cggtggggat ggaggttccc acgggcccgc tggagctccc ccccgcgctg gccgcgacga
     1501 tcgggccctt cgccggcgac cccgtggcga tggcccgcaa atgcgcgggc gactgggggg
     1561 cgcgcttcct ttttctctcc cttgccggct gccatcccga cgcggggggg cagagcgtgt
     1621 ccttcggtgc ggccgtcgtc cgggaggtgc tctccgccac gcccgcccct gtgatcgtgg
     1681 ggggatgcgg cgacgaggaa gtggacgccc ggctcttccc cgccgtcgca gaggcggccg
     1741 gccgaccggt cttcctctcg tacgcggagg agaagaatta ccggctcgtc gcgggcgcgg
     1801 cgatggcgta cggccacggc gtggtctcct ggtcccccat cgacatcaac atggcaaagc
     1861 agatgaacct cctcctcgcg gatctcgggt tccctcccga tcgcgtcctc atcgacccgc
     1921 tgacgggagg tctcgggtac gggatggaat acaccttcac catcgtcgag cggatccgcc
     1981 ttgccggcct c
//
LOCUS       DASVJL010000496         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1498999, whole genome shotgun sequence.
ACCESSION   DASVJL010000496 DASVJL010000000
VERSION     DASVJL010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498999"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VF357_10230"
     CDS             complement(<1..1452)
                     /locus_tag="VF357_10230"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7524571.1"
                     /translation="MMRKMTVSARIRKNLASPDAGKRRSAVEALSLGDERAIYPLIKA
                     LRDSHPGVQDAAMRSLISIGGEVAAWMVLPLLREDSFLRNAAMVILKEIGTDAVSHLP
                     PLLKDKDDDIRKFAIELIADAGGRHLEKALCERLSDDQNPNVRAAAAKALGTLDCWDA
                     LPNLVAALKDIEWVCFNALEALSGMREDAAIEPILVLLSNPSAAIRHSAIEALGEIGS
                     SGAGEALLAHLGNTDRDERAAVLKSLLRIGVPLPGGGFPEDLLGIFLGDDEWKDRLVA
                     LRGLVGIAGEDALLAIFDVAGSLDATEPDEEEILRAIKDLLLRCGSRDALRRLLDDPS
                     LKFRGKAIVAEVVGEMKILEAVPGLAARLRDDVRDVRIAAAQALGRIGGGGAREALVA
                     AIQDSEGHVRKAVVVALGAVGGKEAFEPVLSLLRREEYDDVAKEAVHALFAIDPEAVV
                     ASSGRLDGRARDLVDAFVRGRITGGRAGDPGQEG"
     gene            complement(1503..2204)
                     /locus_tag="VF357_10235"
     CDS             complement(1503..2204)
                     /locus_tag="VF357_10235"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HEX7524572.1"
                     /translation="MSEQVVLDFLPFIRYTARRLSWRLPPAISEEDLVSVGVVGLLDA
                     LRRFREGTVKLKTFAEHRIKGAMLDELRTADILPRGARDRVNAMKSAHGMLERTLGRL
                     PEDPEVAEALGVSLDDYHKTLRESGAALQIRFEDFGPRKDEDGFGDLLQNFPDVEGKD
                     PLSLAERREIRTQLAKVVAELPEKEQLVLSLYYREELTMKEIGKAIGLTEGRVCQIHG
                     QALLRCKARFEVCFA"
     gene            complement(2240..>2636)
                     /locus_tag="VF357_10240"
     CDS             complement(2240..>2636)
                     /locus_tag="VF357_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEX7524573.1"
                     /translation="IVILTNPEPTALTDAYALIKVLFTRYQEKQFRILVNSARNAADA
                     KEVYRKLSVVTDRFLNVSLDYLGYIPYDKSVPDAVRAQKPFLQMFPGGGASRALNALV
                     ARFADPPEANAKGTLQFFIGNLVSMADAR"
BASE COUNT          455 a          878 c          835 g          468 t
ORIGIN      
        1 cccttcctgg cccggatccc ctgccctgcc tcccgtgatg cggccgcgca cgaacgcatc
       61 gacgagatcc cgggcgcgtc catcgaggcg gccggaggag gcgaccaccg cctcgggatc
      121 gatggcgaaa agggcatgaa cggcctcctt ggccacgtcg tcgtactcct cccgccggag
      181 gagcgacaga acgggctcga aggcctcctt cccgcccact gccccgagtg ccaccacgac
      241 cgcctttcga acgtgcccct cggaatcctg gatcgcggcg acgagagcct cccgggctcc
      301 gccgcccccg atccgaccga gggcctgtgc cgcggcaatc cggacatcgc gaacgtcgtc
      361 ccggagccgg gcggcgaggc ccgggaccgc ctcgagtatt ttcatctctc cgacgacttc
      421 cgcgacgatc gccttcccgc ggaacttcag ggaggggtcg tccagcaggc gccggagggc
      481 gtcccgggat ccgcatcgga gcaggaggtc ctttatggca cggagaatct cttcctcgtc
      541 cggctccgtc gcgtcgaggg atcccgccac gtcgaagatc gcgaggagag catcctcgcc
      601 ggcgatcccg acgagacccc ggagggcaac aaggcgatcc ttccactcgt catcgcccag
      661 gaagatgccc aggagatctt cgggaaagcc cccgccgggg agcggcactc cgatccggag
      721 gaggctcttc aggacggcgg ccctctcgtc ccggtccgtg tttccgaggt gggcgagaag
      781 ggcctcgccg gctccggaag atccgatctc tcccagcgcc tcgatcgcgc tgtggcgaat
      841 ggcggcggat ggattggaga ggaggacgag gatcggttct atagcggcgt cctcccgcat
      901 ccccgagagc gcctcgagag cgttgaagca gacccactcg atgtccttca gggcggcgac
      961 gaggttcggc agggcatccc agcagtcgag ggttcccagc gccttggcgg cggcggccct
     1021 cacgttgggg ttttggtcgt cggagagccg ttcgcacagc gccttctcca ggtgtcggcc
     1081 gccggcgtcg gcgataagct cgatggcgaa tttccggatg tcgtcgtcct tgtccttgag
     1141 gaggggcggg agatgcgaaa cggcgtccgt cccgatctct ttcaggatga ccatcgcggc
     1201 gttccggagg aaggaatcct cccgaaggag ggggagaacc atccaggccg cgacttcccc
     1261 gccgatggag atcagcgagc gcatcgcggc gtcctggact ccggggtggg agtcccgcag
     1321 cgccttgatg agggggtaaa tcgcccgctc gtccccgagc gaaagcgcct cgacggctga
     1381 ccgccttttc ccggcgtccg ggctcgcgag gtttttcctg atccttgccg acacggtcat
     1441 cttcctcatc attatcctta tcggtgtaaa ccgaaaagtc ttaaatgaaa ctgcaacttt
     1501 gttcaggcaa aacagacctc gaaccgggcc ttgcaccgaa gaagcgcctg cccgtgaatc
     1561 tggcacactc ttccctcggt caacccgatc gccttgccga tctccttcat cgtcagctct
     1621 tcacggtaat agagggacag gacgagttgt tccttctccg ggagctccgc gaccactttc
     1681 gcgagttgtg tccgaatctc cctcctttcc gccagcgaca agggatcttt cccttccacg
     1741 tcgggaaagt tctggaggag atcgccgaat ccgtcttcgt cctttcgcgg accgaagtcc
     1801 tcgaaccgga tctggagagc ggcgccgctc tcccgcaggg tcttatggta atcgtcgagc
     1861 gagacgccga gagcctcggc aacctcggga tcctccggga gccgcccgag cgtccgctcg
     1921 agcatgccgt gggcgctctt catggcgttc acccgatccc tcgctccccg aggcaggatg
     1981 tcggccgtcc ggagctcgtc gagcatcgca cccttgatcc ggtgctccgc gaacgttttg
     2041 agcttcaccg tcccttcccg gaatctgcgc aacgcatcga ggagaccgac gacacccacg
     2101 ctgacgagat cctcttccga gatcgccggc ggcagccgcc agcttagacg gcgtgcggtg
     2161 taacggatga acgggagaaa atcgaggacc acttgttccg acacgtctgc ctccacctct
     2221 gccactggac gagcggctct catctcgcgt ccgccatgga gacgaggttg ccgatgaaga
     2281 actggagcgt acccttggcg ttcgcctccg ggggatcagc gaagcgcgcg acgagcgcgt
     2341 tgagggcccg ggacgccccc cccccgggga acatctggag aaacggcttc tgcgcccgga
     2401 ccgcatcggg aaccgacttg tcgtaaggga tgtagccgag atagtcgagg gagacgttca
     2461 ggaaccggtc ggtgacgacc gagagcttcc ggtaaacctc cttcgcatcc gccgcgttac
     2521 gagcggagtt cacgaggatc cggaactgtt tctcctggta ccgcgtgaaa agcaccttga
     2581 tgagggcgta ggcgtcggtc agggccgtcg gctcgggatt cgtcaggatg acgatc
//
LOCUS       DASVJL010000497         6564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1122252, whole genome shotgun sequence.
ACCESSION   DASVJL010000497 DASVJL010000000
VERSION     DASVJL010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122252"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..835
                     /locus_tag="VF357_10245"
     CDS             98..835
                     /locus_tag="VF357_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX7524574.1"
                     /translation="MNVAILSDIHANFDALSAVAAELALRRPDRIYHLGDLAGYNAQP
                     EECVRWAMANAPAGVSGNHDAVACGRATGRNFHAAARKAALWSRARISAEGRDYLSLL
                     PVQRFIEGGALLVHGAPSDPGRYVDSVDHAAQELSTLFASVATGPIFFGHTHVAGGFV
                     LRGDGRVKPVPAKEFRLLPGERALLNPGSVGQPRDGDRDASFLLFDTAAGTVTWIRVP
                     YDFEAARRKVLEAGLPREFADRLRDGT"
     gene            complement(1014..3038)
                     /locus_tag="VF357_10250"
     CDS             complement(1014..3038)
                     /locus_tag="VF357_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX7524575.1"
                     /translation="MSRNLDPGEPAGFPWKKLLLILFVAGLCFVLGAGAGIVLLAKFG
                     DFPPIESAAAYRPSVTSKIFDRNNRVVGEIYIEKRTVVPFETIPPHVVNAFVAAEDAN
                     FFKHRGVDFVAIARAIVKDVVSGGFAQGASTITQQTVKSLFLTPEKSISRKLKELILA
                     YRIERKLSKEEILYLYLNQIYLGDGAYGVESAAQTYFGRGVSTLTVAEGALLAGLAQA
                     PTRYSPRSHIDKARARQRYVLRRMAEVGFIDKKGAESAYDARLALAPPSTFRSKAAYF
                     LEYVRNYLQDRYGAEAMNRSEFRIYTTIDKRLQEAAYDAVTQGVRRIEEANKYRGLQG
                     ALLCMDPNSGGVLAMVGGVDFGASQFNRALQARRQPGSAFKPVIYAAAIDKGKTLVST
                     VDDSPIEFARSETEMWKPKNYDDSFLGPIPLLESLAKSRNLATVRLLNEIGVDTAIGM
                     ARNLGIQSPIERNLSIALGSSGVTPLEMVAAYSTFAAGGQRPTPFFIREVQDGQGRVL
                     ERTDPKVVQAIATETAFLTIRLMQEVLRTGTGASAKGLSPNLAGKTGTTNENTDAWFI
                     GGSPDLIAGVWVGFDTPASLGKRQTAASVALPIWAHFFGQALTYVPDREFPAPAGITF
                     ARVDPSTGKAVPPGSVEGMLLPFKLGTVPETGGPVGRPSPSRRGTGDDLL"
     gene            3197..3820
                     /locus_tag="VF357_10255"
     CDS             3197..3820
                     /locus_tag="VF357_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524576.1"
                     /translation="MRKIGILGLAGVFALVLAGCGGGDDHHTVVIHSFSDQPVDGDIG
                     FTSPATFSFSQANVTRNVQYGINSGGTEFRAFLDFPLDGSNGGGVVPLNAVIVSADIE
                     VFVDNVSFSSSVPTLLDLVTFPVTGLTSADFDSAPLLTRSPFDLRTSDINNFVRIDVT
                     STMVEAQRQGVRNLQLRFLLDFSAATGLVVLDDGAQGSAPLLTVEYR"
     gene            3907..4674
                     /locus_tag="VF357_10260"
     CDS             3907..4674
                     /locus_tag="VF357_10260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7524577.1"
                     /translation="MPATRTRNKLADLGAVAAGLAHEIRNPLNSLYINGQLLEEMLSE
                     LPEEGVPQKGDLLSLARANLKVTQRLNDTLSGFLRFARPPAMELALVDLNRIVSETLR
                     FLEMELAYRGISLKTKLYPTPLPILADEKLLKQALLNLLLNAQEAVEKEEKVIRVTTG
                     VRAGRPFVRIRDNGRGIPASDRRHIFRLFFTTRKNGSGLGLPIVRQIVRQHGGAIYIR
                     SREGQGTTMTVALLFEEQFRAMYPGRLPLALPQGGRP"
     gene            4671..6041
                     /locus_tag="VF357_10265"
     CDS             4671..6041
                     /locus_tag="VF357_10265"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7524578.1"
                     /translation="MNQKGKGRVFVFDDDADSLQSVVAALRRDGFDVFPFADPREGLA
                     RIEAEGGDVVVTDLRMPGLTGLEVLRHVTMKIPEVPVVILTAYGTVEGAIEAVRAGAS
                     DFLLKPVEIPRLRAAVFKALKERGMHREIERLRKEAGRPLGIEGIVGTSRSMEEVLRK
                     IRLVAPTRMNVLITGESGTGKELVARGIHGLGPRADQPFLPLNCAAIPETLLETELFG
                     HEKGAFTGATASRPGKFENAEGGTLFLDEVGDVSPGIQSKLLRAIEQKEVMRVGGSQV
                     IHADVRIIAATNQDLKGRVEAKEFREDLYYRLNVFNIVVPPLRERREDIPKLCDHFLA
                     LIGKENGFPPKRLSPAALKSLLACRWPGNVRQLRNALETATLVAQGEAIAPDDLPPEV
                     TQAVLPPTAGEPIPLPASRTLGEMERDAIRAALVQTAGNKTLAAKLLGIGLRTLHRKV
                     KEYGIE"
     gene            6346..>6564
                     /locus_tag="VF357_10270"
     CDS             6346..>6564
                     /locus_tag="VF357_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX7524579.1"
                     /translation="MAKIIGIDLGTTNSVVAVMEGSEPKVLINEEGGRLTPSVVGFGK
                     DGQILVGQVAKRQAVLNPENTVFSIKRFM"
BASE COUNT         1090 a         2175 c         2178 g         1121 t
ORIGIN      
        1 tcgacatctt catggacctc gtccgggaat attccatcag gaagcggacg gtccggacgc
       61 ttccccccgg actggggaag gccgcggccg tcagcgcttg aacgtcgcca tcctctcgga
      121 catccacgcg aacttcgacg ccctctcggc ggttgccgcg gagctcgccc tccgccggcc
      181 ggaccggatc tatcacctcg gggacctcgc cgggtacaac gcccagccgg aagagtgcgt
      241 ccgctgggcg atggcgaacg cgccggcggg cgtttccggg aaccacgacg ccgtcgcgtg
      301 tggccgcgcc acgggccgga acttccacgc cgcggcgcgg aaggcggccc tctggtcgcg
      361 ggcccggatc tccgcggagg gccgcgatta cctttccctc cttcccgtcc agcgtttcat
      421 cgagggcggt gcgctcctcg tccacggagc ccccagcgat cccggcaggt acgtcgattc
      481 cgtcgaccac gctgcccagg aactttccac cctcttcgcg tccgtggcga cggggccgat
      541 cttcttcggg catacccacg ttgcgggcgg gtttgtccta cggggggacg gccgggtgaa
      601 gcctgttccc gcgaaggagt tccgcctttt gccgggtgag cgggcgctcc tcaacccggg
      661 gagcgtcggg cagccgaggg acggcgaccg ggacgcctcc ttcctgctgt tcgataccgc
      721 cgccggtacg gtgacctgga tccgcgtgcc gtacgatttt gaggcggccc gccggaaagt
      781 gctcgaggcg gggctcccgc gggagttcgc cgaccggctg cgggacggga cgtgagcctg
      841 cgctggtgca gcgtctcgca aatatcctgg cattcgagcg ccgctgcatc cactccagct
      901 tcgttgcgct agttcgacgt actctcccgg tacgccttca ctagcgcgcc ttgctggcgc
      961 ggcgcatcga cgatctcgat gcgtcaggct atttgcgaga cgctgcacca gatctacagc
     1021 aggtcgtcgc ccgttccgcg ccgtgatggg gagggtctcc cgacggggcc tccggtctcc
     1081 gggaccgtgc ccaatttgaa ggggagcaac atcccctcga cgcttcccgg cggcaccgcc
     1141 ttgccggtcg acgggtccac tcgggcgaag gtgatccccg cgggagcggg gaactcccgg
     1201 tcggggacgt aggtcagcgc ctgcccgaag aagtgcgccc agatcggcag cgccaccgag
     1261 gccgccgtct ggcgcttccc cagcgacgcc ggggtgtcga accccaccca cacccccgcg
     1321 atcaggtccg gcgatccgcc gatgaaccag gcgtccgtgt tctcgttcgt cgttcccgtc
     1381 ttccccgcga ggttcggcga gagccccttc gccgaggcgc cggtcccggt gcgaagaacc
     1441 tcctgcatca gccggatggt gagaaaggcg gtctccgttg cgatcgcctg cacgaccttg
     1501 gggtcggtcc gttccagcac gcgcccctgc ccgtcctgca cctcccggat gaagaacggc
     1561 gtcggccgct gtccgccggc ggcgaaggtc gagtacgcgg cgaccatctc gaggggggtc
     1621 acgcccgacg acccgagggc gatggagagg ttccgctcga tgggcgactg gatgccgagg
     1681 ttgcgggcca tcccgatcgc cgtgtccacg ccgatctcgt tcaaaagacg gacggtcgcc
     1741 aggttgcggg acttggccag cgactcgagc agcgggatgg ggccgaggaa cgaatcgtcg
     1801 taattcttcg gcttccacat ctccgtctcg ctcctcgcga actcgatcgg cgagtcgtcc
     1861 accgtggaga ccagcgtctt ccccttgtcg atggcggcgg cgtagatgac cggcttgaag
     1921 gcggagcccg gctgccgacg cgcctggagc gcccggttga actgcgacgc gccgaagtcg
     1981 accccgccca ccatcgccag caccccgccc gagttcgggt ccatgcacag cagcgcgccc
     2041 tggagccccc ggtacttgtt cgcctcctcg atccggcgga cgccctgcgt cacggcgtcg
     2101 tacgccgctt cctgcaaccg cttgtcgatc gtcgtgtaga tccggaactc gctccggttc
     2161 atcgcttccg cgccgtacct gtcctgcagg tagttgcgga cgtactcgag gaagtaggcg
     2221 gccttcgaac ggaaagtgga cggaggggcg agggcaaggc gcgcgtcgta cgcactctcg
     2281 gcgcccttct tgtcgatgaa tcccacctcc gccatccggc gcagcacgta ccgctgccgt
     2341 gccctcgcct tgtcgatgtg actccgcggc gagtagcggg tgggcgcctg ggccaggccc
     2401 gcgaggagcg ccccttccgc gacggtgagg gtggaaaccc ccctcccgaa gtaggtctgc
     2461 gccgccgact cgaccccgta cgccccgtcc ccgaggtaga tctggttcag gtagaggtag
     2521 aggatctcct ctttcgacag cttcctctcg atccggtagg cgaggatcag ctccttcagc
     2581 ttgcgggaga tgctcttttc cggggtgagg aagagggact tgaccgtctg ctgggtgatc
     2641 gtgctggccc cctgtgcgaa tccgccggaa acgacgtcct tgacgatcgc tcgggcgatg
     2701 gcgacgaagt ccactcccct gtgtttgaag aagttcgcgt cctcggcggc gacgaacgcg
     2761 ttgacgacgt gcggggggat ggtctcgaag gggaccacgg tccgcttctc gatgtagatc
     2821 tccccgacga cgcggttgtt ccggtcgaag atcttcgagg tgacgctggg ccggtacgcc
     2881 gcggccgact cgatcggcgg gaaatccccg aatttcgcca gcaggacgat cccggccccc
     2941 gcccccagga cgaagcacag ccccgcgacg aacaggatca ggagcagttt tttccacggg
     3001 aagccagcgg gctccccggg atcgagattc cgggacaacg gtccgcctct cttgttcggg
     3061 gaatatactc agtcgattat agcaggccgg cgggaaaggt ggggatgagg gcatccggcg
     3121 catcaggtga atacttttcc cccggtgagc gtaaaatata gtgtatcgga aaacggccga
     3181 cacgggagga acggcgatgc ggaaaatcgg gatcctcggg ctcgcgggcg tcttcgcgtt
     3241 ggtccttgca ggctgcggtg gaggggacga tcaccacacg gtcgtcatcc actcctttag
     3301 cgaccagccg gtcgatggag acatcgggtt tacctctccc gccacgttct ccttctcgca
     3361 ggccaacgtc acgaggaacg ttcagtacgg catcaattcc ggggggaccg aattcagggc
     3421 gttcctggat ttccccctcg acggatccaa cggaggcggg gtggttcctc tgaatgccgt
     3481 gatcgtttcg gcggatatcg aggtcttcgt ggacaacgtc agtttctcct cgtccgtgcc
     3541 gacgcttctc gatctggtca cgtttcccgt cacgggcctg accagcgcgg atttcgactc
     3601 ggctccgctg ctgacacggt cgccgttcga tcttcgtacc tcggacatca ataatttcgt
     3661 ccggatcgac gtcacgtcga cgatggtgga agcgcagcgc cagggagtgc gcaatctgca
     3721 gcttcggttc ctcctggact tctcagcggc aacggggttg gtggtgctgg acgacggcgc
     3781 gcaggggagc gcccccctcc tgaccgtgga atatcggtaa ccgggatcgg atccccgccc
     3841 gggatggatg aaatgtgcaa ccggtgcggc gttccggggg tccccgtttg atgtagaatc
     3901 gtcctcgtgc cggccacgag aacccgtaat aagctcgcgg acctgggcgc cgtcgcggcg
     3961 ggcctggccc acgagatccg caatccgctg aactcccttt acatcaacgg ccagctcctc
     4021 gaggagatgc tttccgagct gccggaggag ggcgttccgc agaaggggga cctcctctcc
     4081 ctcgcccgcg ccaacctcaa ggtcacgcag cggctgaacg acacgctctc gggcttcctg
     4141 cgcttcgccc ggcccccggc gatggagctt gccctggtcg acctcaaccg gatcgtgtcg
     4201 gagacgctcc ggttcctcga gatggaactc gcgtaccggg ggatctccct caagacgaag
     4261 ctttacccaa cgcccctgcc gatcctggcc gacgagaaac tgttgaagca ggcgctgctc
     4321 aaccttctgc tgaacgccca ggaagcggtg gagaaggagg agaaggtgat ccgcgtgacc
     4381 accggtgtgc gcgcggggcg gccattcgtg cggatccgcg acaacggccg cgggatcccg
     4441 gcatccgacc gccgccacat ctttcgcctc ttcttcacaa cccggaagaa cggcagcggc
     4501 ctggggcttc cgatcgtccg gcagatcgtg cggcagcacg ggggggcgat ctacatccgc
     4561 agccgggagg ggcaagggac cacgatgacc gtcgcgctcc tgttcgagga gcagttccgg
     4621 gcgatgtatc ccggccgcct gccgctcgcg ctgccgcagg gggggcgtcc atgaaccaga
     4681 aggggaaggg gcgcgtcttc gttttcgacg acgacgccga cagcctccag agcgtcgtcg
     4741 ccgcgctgcg ccgcgacggg ttcgacgtct tccccttcgc cgacccgcgg gaaggattgg
     4801 cgcgcatcga ggccgagggt ggggacgtcg tcgtcaccga cctgcgaatg cccggcctga
     4861 ccggcctcga ggtgctccgt cacgtcacga tgaagatccc ggaggtcccg gtggtcatcc
     4921 tgacggccta cggcaccgtc gagggggcga tcgaggcggt gcgcgccggc gcctccgatt
     4981 tcctcctcaa gcccgtggag attccccgtc tgagggccgc cgtcttcaag gcgctcaagg
     5041 agcgcgggat gcaccgggag atcgagcggc tccgcaagga ggccggccgg ccgctcggga
     5101 tcgaggggat cgtcggaacc tcccgctcga tggaagaggt cctgcggaag atccgcctgg
     5161 tcgctccgac acggatgaac gtcctgatca ccggggaaag cggcacgggg aaggaactgg
     5221 tcgcgcgggg gatccacgga ctgggccccc gcgccgacca gccgttcctg ccgttgaact
     5281 gcgccgcgat ccccgagacg ctcctcgaga ccgagctctt cggccacgag aagggggcgt
     5341 tcacgggggc gaccgcctcc cggcccggga agttcgagaa cgccgaagga gggacgctct
     5401 tcctcgacga ggtaggggac gtgtccccgg gaatccagtc gaagctcctg cgcgccatcg
     5461 agcagaagga ggtcatgcgg gtcggcgggt cgcaggtgat ccacgcggac gttcggatca
     5521 tcgcggcgac gaaccaggat ctgaaaggac gggtagaggc gaaggagttc cgcgaagacc
     5581 tctactaccg gctgaacgtc ttcaacatcg tcgtcccgcc gctacgggaa cgccgcgagg
     5641 acatcccgaa actgtgcgac cacttccttg cgctgatcgg gaaagagaac gggtttcccc
     5701 cgaagcggct ttcccccgcg gcgctgaaaa gcctgctcgc ctgccggtgg cccgggaacg
     5761 tccgccagct gcgcaacgcc ctggagaccg cgaccctcgt cgcgcagggg gaggcgatcg
     5821 cgcccgacga ccttcccccc gaggtgacgc aagcggtcct cccccccacg gccggcgaac
     5881 cgatccccct gcccgcctcc cgcaccctcg gcgagatgga gcgggacgcc atccgcgccg
     5941 ccctcgtcca gaccgcaggg aacaagaccc tggcggcgaa gctcctcggc atcggcctgc
     6001 gcaccctcca ccgcaaggtc aaggagtacg gaatcgaata ggcgggaagg aggctagggg
     6061 gggacatacc cgtcaggggg gggaaatttc tggcctgacc cgcgctgcgc cagaatggca
     6121 ctatacgcct tcttgctgtg ccttaatggc acatatctcc cgggaccctc cttcgcgaag
     6181 ttatccttcc ttataatatt tccttatgga aacggatatt tgtcgatata ttggaatcca
     6241 ggcacgactg gcacgagact tgtttgattt taagcagaaa ggttcgtgga gctgcgacgc
     6301 cggaacacgc ttgaccaaaa tgaataagag gaaggagcaa cgaagatggc gaagatcatc
     6361 gggatcgacc tggggacgac gaactccgtc gtggcggtga tggaggggag cgagccgaag
     6421 gtactcatca acgaggaggg gggccggctc accccgtcgg tggtcgggtt cggcaaggac
     6481 ggccagatcc tcgtcggcca ggtggcgaag cgccaggcgg tgctgaaccc ggagaacacc
     6541 gttttctcca tcaagcggtt catg
//
LOCUS       DASVJL010000498         6647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_935176, whole genome shotgun sequence.
ACCESSION   DASVJL010000498 DASVJL010000000
VERSION     DASVJL010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935176"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VF357_10275"
     CDS             complement(<1..571)
                     /locus_tag="VF357_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524580.1"
                     /translation="MSSTADKIAHLRKTAFEHPEYIDVLRPFEEIFAYVDGKEAGTGI
                     RFAVPGGDGSQRLDGGLPLLSPEALSVDRDAATAFLSGLLDVLRRVGREGHADLDRIG
                     GGLSDGSLDLSSLYIACLLRKRDVVDQAAAALSVQPPLLTFILEIPLKIALERISSSL
                     PPERFDGWKEGYCPVCGSRAGMAELSGDEG"
     gene            complement(600..1214)
                     /locus_tag="VF357_10280"
     CDS             complement(600..1214)
                     /locus_tag="VF357_10280"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX7524581.1"
                     /translation="MGKKYVERFNTAERVLHWFVAASFFTLLLSGLGLYSRLFHGYFD
                     LFGGGVGAILAHKYAGAVFFISSLMLFLNHAKEMFTFDEEDRKWFRSLGGYLSKNPDH
                     INSGKFNAGQKGLGIFMGIATLLLGITGVINWIPESFPRGLVQFSLFLHGLLFVAFVM
                     LMVVHIYLTTIGNPGTLEGMLYGNVRRIWARTHHPKWYKEVEGE"
     gene            complement(1207..1392)
                     /locus_tag="VF357_10285"
     CDS             complement(1207..1392)
                     /locus_tag="VF357_10285"
                     /inference="COORDINATES: protein motif:HMM:NF020725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta
                     transmembrane domain-containing protein"
                     /protein_id="HEX7524582.1"
                     /translation="MYGLPADPSIPTSIFLWKDVVRPLGILGFWGSVAAVVVHYVTFG
                     SRQLEEDGKGKGGDANG"
     gene            1596..2612
                     /gene="epmA"
                     /locus_tag="VF357_10290"
     CDS             1596..2612
                     /gene="epmA"
                     /locus_tag="VF357_10290"
                     /inference="COORDINATES: protein motif:HMM:NF006828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEX7524583.1"
                     /translation="MPREGTERETWRRLASGELSWEALRARARVHEEIRAFFRDRGFL
                     EVDPPIAQAYPNIDPNIFPVRIADASGKGARFYLHTSPEFAMKKLLAAGSGNIFFLGK
                     VFRDREGSPLHSPEFTMLEWYRVGGDANAVMRDVEELVRTLARADAFEAVVHGNGREI
                     PVDGPWPRWELDDAFRELLGVGMAGEKELRGALDRKGLRPGADEPWEDLFYRACLDVV
                     EPALAERGACFLTGYPAALGAMARRRPGRPEVSERFEGFVAGVELVNGYEELTDPAEQ
                     EARLLALTDHHRRSTGETLPVDPGFLDALRSGLPPCAGAALGVDRLVMLLLGKNDIAD
                     GMYR"
     gene            2609..3778
                     /locus_tag="VF357_10295"
     CDS             2609..3778
                     /locus_tag="VF357_10295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7524584.1"
                     /translation="MNSSAVLLFLLSFAHMCNDIAQGALPALLPFLKERFDLSYAAAG
                     SLLMVAYLTSSVIQPLFGYVTDRRPFPILLPLGCAISGVGLALIPFSPSFGWLVAIVM
                     FAGVGTAAFHPEGFKATACIASERRATGMSFFSVGGNLGFAIGSPAAIFLVSRYGLPG
                     ASGLFLPTAVAALLLLPALPTIRNRIESVSSAPRKSAMSGVEHPLYAVSLIILIVVLR
                     SWTQLGLATYIPFLYQAQLKSDPAYVATLLFFFLGAGTVGTVIGGPLADRFGHRRLLF
                     FSVALQIPLIFLFLRSSGWMVFASAALLGGTIVSTFSVTIVMAQELFPKRMATASGAI
                     AGFAIGTGGVGVTLIGAVADRYGVPAATNLINVLPAIGLLLALLLPLPWKGGEER"
     gene            3787..5190
                     /locus_tag="VF357_10300"
     CDS             3787..5190
                     /locus_tag="VF357_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7524585.1"
                     /translation="MPGNIETNAVGIRVLLSVGLGTFMSALDASVVNTVLPVIRADYH
                     VPVEVIQWVSTTYLLIMGSLLLTFGRLGDLRGHKRVYLAGLVVFVPASVLCGISPSVE
                     MLIASRVLQGIGAAIVVSTAPAILIGSVHPSRMGQALGLQAAMTSIGLALGPSLGGWL
                     TDQWGWRTIFFMNVPVGVAAFAIGHRYIPEEKSPPLREETFDRVGALLFLAAFLVLQV
                     ALSRGNDWGWLSAPTLGVFAGSAALGYLFLRQETTATSPMLHLALFRNRLFSFSVGSA
                     LVNYICMASVLFLVPFYLIQGRGFSAAKAGMVMTAQFLSMVVAAPVSGRIADRVGSRI
                     PATTGMAILAVGLFILSAMDGESPLHLTVLGLVVTGVGVAIFVTPNNSAMLGTAPPGR
                     KGIASGILATSRLMGMATGVSLAGAILAGVVGGRSAHGIPPERVFKAIHLGFLAAGVM
                     ASGGTVLTAARSEGRTH"
     gene            5187..6449
                     /locus_tag="VF357_10305"
     CDS             5187..6449
                     /locus_tag="VF357_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7524586.1"
                     /translation="MTPYDERYRRILDLGKSIKANREEFIRRAVKDIQFTYRDTAQEV
                     DTSLNRLTMYTEAKPLLEGRRPLGGEGSTVALMLSYNGSAWLNTAITSIYMVGNHVNV
                     KFSSMGSGLSALTESIYKPIFGDDVRFSRESGKRFLEEALKDTAVSAVVVFGSDVNFL
                     AYEPAFREAGKKMVFEGAGQDPFIVFPDADLDLALSDLVTAKFMYSGQTCTAPKRIFI
                     HQSIYGEFLERFVERTVKPVVGPPEDPRTEISPVASRLAVARIRGQLKEAVAMGAKVV
                     CGGMIDGNLIHPTVVRDATDAMSGMREEVFGPVAFTTPFDTKEEVVRRAKGHKYGLRA
                     ALFGGKEAEEAAGELAGDPYCHPVPAYTFGKFGTVVRNQTRTDSWKGAFVVKAVGGYG
                     YSGWIWETVGGKFRIKQGPKLLSIETSI"
BASE COUNT         1079 a         2174 c         2169 g         1225 t
ORIGIN      
        1 tcccctcgtc cccggaaagt tcagccatcc cggcgcggga gccgcacacg ggacaatacc
       61 cttccttcca gccatcgaac cgttccgggg gaagagagga ggaaatgcgc tcgagggcga
      121 tcttcaacgg aatctcgagg ataaaggtga gcaacggcgg ctggacggaa agggcggccg
      181 ccgcctggtc cacgacgtcc cgttttcgga gaagacaggc gatgtacagg gaggaaaggt
      241 cgagggagcc gtccgaaagc cccccgccga tccgatcgag atccgcatgt ccctccctgc
      301 cgacgcgccg caggacgtcg agcaaccccg acaggaaggc ggtcgcggcg tcccggtcca
      361 ccgagagcgc ctcgggggag aggagcggaa gacctccgtc cagccgttgc gacccgtccc
      421 cccccgggac ggcgaagcgg atccccgtcc ctgcctcctt cccgtcgacg tacgcgaaga
      481 tctcctcgaa cgggcggagc acatcgatgt actccgggtg ctcgaacgcg gtcttccgga
      541 ggtgcgcgat cttgtccgcc gtactgctca tcccgccccc cgaagccgct tcccgccgat
      601 tattcgccct ctacctcctt ataccacttc gggtggtgcg tccgcgccca gatccttcgg
      661 acgttgccgt aaagcatccc ctcgagcgtc cccgggttgc cgatcgtggt gaggtagatg
      721 tgcaccacca tcagcatcac gaacgccacg aacaggaggc catggaggaa cagcgaaaac
      781 tggacgagcc cccgcggaaa cgactcgggg atccagttga tgacccccgt gatgccgagc
      841 aggagggtcg cgattcccat gaagatgccg agccccttct gtcccgcgtt gaacttcccg
      901 ctgttgatgt gatccggatt cttcgacagg tagcccccca gcgaccggaa ccacttccgg
      961 tcctcctcgt cgaaggtgaa catctccttc gcgtggttca ggaagagcat cagggaactg
     1021 atgaagaaga ccgcccccgc gtacttgtgg gccaggatcg cccccacccc gcccccgaac
     1081 aggtcgaagt agccgtgaaa gaggcgggag tagagtccca gtcccgacag gagcagggtg
     1141 aagaacgacg cggcgacgaa ccagtggagc acccgctcgg ccgtgttgaa ccgctcaacg
     1201 tattttttac ccattcgcgt cccctccctt cccctttcca tcctcctcga gctggcgcga
     1261 tccgaaggtc acatagtgga ccaccacggc ggcgacgctg ccccagaagc cgaggatccc
     1321 gagcggcctc acgacgtcct tccacaggaa gatcgacgtc gggatcgacg ggtccgccgg
     1381 caggccgtac acctcgggtt tctcctcgag tgcgtagacg acgccatgct gcgaccgctt
     1441 gggctgtcga gagaagaaaa ggagtccctc cgggagttcc tcgccaccgg gctttccggc
     1501 aggatgccgg aaagccgccc gcccgccatc ccctgaggtc tcttgccgga aacgatgtcc
     1561 ataaggtttt atccgtgggt atgcgataat tacggatgcc tcgggaagga acggaacggg
     1621 agacgtggcg gcggctcgcg agcggtgagc tgtcgtggga ggcgcttcgg gcgcgggccc
     1681 gggtccacga ggagatccgg gccttcttcc gggaccgggg gttcctcgag gtggaccccc
     1741 cgatcgcgca ggcgtacccg aacatcgacc cgaatatctt cccggtgagg atcgccgacg
     1801 cctccggcaa gggggcgcgg ttctacctgc acacctctcc cgaattcgcg atgaagaagc
     1861 ttcttgctgc cggatcgggc aacatcttct ttctcgggaa ggtgttccgg gatcgtgaag
     1921 ggtcgcccct ccactctccc gagttcacga tgctcgaatg gtatcgggtg ggcggcgatg
     1981 caaatgccgt gatgcgggac gtcgaggagt tggtccggac gctggcccga gcggatgcct
     2041 tcgaggcggt cgttcacggc aacgggaggg agatcccggt cgacgggccg tggccccggt
     2101 gggaactcga cgacgcgttc cgggaactcc tcggtgtcgg catggcgggg gagaaggagt
     2161 tgcgcggagc gctcgaccgc aaggggttgc gtccgggagc ggacgaaccg tgggaggacc
     2221 tcttctaccg ggcctgcctc gacgtggtgg agccggcgct cgccgaacgg ggcgcctgtt
     2281 tcctcacggg ctaccccgcg gccctcgggg cgatggcgcg ccgtcgcccc ggacgccccg
     2341 aagtgtcgga gcggttcgag ggattcgtgg ccggtgtcga gctggtcaac gggtacgagg
     2401 agctgaccga ccccgcggag caggaggccc gcctgctggc gctgaccgac caccaccggc
     2461 gatcgaccgg tgagacgctt cccgtggacc cggggttcct cgacgcgctg cgtagcggcc
     2521 tccccccctg cgcgggtgcc gcactggggg tcgaccggct cgtcatgctc ctgctgggga
     2581 agaacgacat cgcggacggg atgtaccggt gaactcctcc gccgtcctgc tcttcctcct
     2641 ctccttcgcg cacatgtgca atgacatcgc gcagggggcc ctgccggcac tgctcccgtt
     2701 cctcaaggaa cggttcgacc tgtcgtacgc agccgcggga tccctgctca tggtggcgta
     2761 cctcacctct tccgtgatcc agccgctgtt cggatacgta acggaccggc gccccttccc
     2821 gatcctgctg ccgctggggt gcgcgatctc cggcgtcggg ctcgcattga ttccgttctc
     2881 cccgtcgttc ggctggctgg tggcgatcgt catgttcgca ggggtgggca ccgcggcgtt
     2941 ccacccggag gggttcaagg cgaccgcctg catcgcgtcg gagcgacgcg cgacggggat
     3001 gtccttcttc tccgtgggtg ggaacctcgg gttcgccatc ggctccccgg cggcgatctt
     3061 cctcgtctcc aggtatgggc tcccgggggc gtccggcctc tttctgccga ccgccgtcgc
     3121 cgcgctcctg ctcctccccg ccctgccgac catccggaac cggatcgaat cggtatcctc
     3181 cgctccgcgg aagagcgcga tgagcggcgt ggagcatccc ctctacgccg tgtcgctcat
     3241 catcctgatc gtcgtgttgc gctcctggac gcagctgggg ctcgccacct atatcccttt
     3301 cctctaccag gcgcagttga agagcgaccc ggcgtacgtg gcgaccctcc tcttcttctt
     3361 cctcggcgcg ggcaccgtcg gcaccgtgat cggggggccg ctggcggacc ggttcgggca
     3421 ccgtcggctc ctcttcttct ccgtcgccct gcagatcccc ctgatcttcc ttttcctgcg
     3481 gtcgtcgggg tggatggtct tcgcatcggc cgcgctcctg ggcgggacga tcgtttcgac
     3541 cttctcggtc acgatcgtga tggcccagga actgttcccg aagcggatgg ccacggcctc
     3601 tggcgccatt gccgggttcg ccatcggcac gggcggtgtc ggcgtcaccc tgatcggagc
     3661 ggtcgccgac cggtacggcg tcccggcggc cacgaacctg atcaacgtcc tccccgcgat
     3721 cggcctcctg ctcgccctgc tcctcccgct gccctggaag ggcggggagg agcgctgacg
     3781 gacttcatgc cggggaatat cgaaacgaat gccgtcggca tccgggtgct tctgtccgtg
     3841 ggtctcggga cgttcatgtc cgctctcgat gcaagcgtgg tcaacacggt gcttcccgtg
     3901 attcgggcgg actaccatgt tcccgtcgag gtgatccagt gggtgtcgac gacttacctg
     3961 ctgatcatgg gatcgcttct cctcaccttc gggcgcctgg gggacctccg ggggcataag
     4021 cgcgtttatc ttgccgggct cgtggtcttt gtgccggcgt cggtcctgtg cggaatctcc
     4081 ccttcggtcg agatgctgat cgcctcccgc gtcctccagg ggatcggggc cgccatcgtg
     4141 gtctccaccg cgcctgccat cctcatcgga agcgtgcacc catcgcggat gggacaggca
     4201 ttgggactcc aggccgccat gacatccatc gggcttgccc tggggccttc cctgggcgga
     4261 tggctcacgg accaatgggg atggagaacg atcttcttca tgaacgtccc cgtcggcgtg
     4321 gccgctttcg cgatcgggca ccggtacatc cccgaagaga aaagcccgcc gctgcgggag
     4381 gagacgttcg atcgggtcgg ggccctcctt ttcctcgcgg cgttcctcgt cttgcaggtc
     4441 gcgttgagcc gcgggaacga ctggggatgg ctctcggcgc cgaccctcgg cgtcttcgcc
     4501 ggaagcgccg cccttggata cctgttcctg cggcaggaaa cgaccgcaac gtctcccatg
     4561 ctgcatctgg ccctcttcag aaaccgcctt ttctcctttt cggtggggag cgccctggtg
     4621 aactacatct gcatggcaag cgtgctcttc ctggtcccct tctacctcat ccagggacga
     4681 ggcttttcgg cggcgaaggc gggcatggtc atgacggccc agtttctttc gatggtggtg
     4741 gccgctcccg tcagcgggag gatcgccgat cgcgtcggct cccgtattcc cgccacgacg
     4801 ggcatggcga tcctggcggt cggacttttc atcctttccg ccatggacgg ggagtcccct
     4861 ctccacctca cggtgctcgg cctggtggtg acgggagttg gggtggcgat tttcgttacg
     4921 cccaacaata gcgcgatgct cgggacggcg ccgccgggac ggaaggggat cgcttcggga
     4981 atcctcgcca cgtcgcggct gatggggatg gccacaggcg tcagtctggc gggcgcgatc
     5041 ctcgccggcg tcgtcggcgg tcggtcggct cacgggatcc ccccggaacg ggtcttcaaa
     5101 gcgatccatc tcgggttcct cgcggccggc gtgatggcat cggggggaac ggttcttacg
     5161 gcggcgcgta gcgaagggag gacgcattga caccgtacga tgaacggtac cggaggatcc
     5221 tcgacctggg caagagcatc aaggcgaaca gggaagagtt catccggcgg gcggtcaagg
     5281 acatccagtt cacctacagg gatactgcgc aggaggtgga cacctctctc aaccggctga
     5341 cgatgtacac cgaggccaag cccctgctcg agggaagaag acctcttggt ggagaagggt
     5401 cgaccgtcgc cctcatgctg tcctataacg gcagcgcctg gctcaacacc gcgatcacct
     5461 cgatctatat ggttggaaat cacgtgaacg tgaagttttc ctcgatggga tccggcctct
     5521 ccgccctcac cgagtccatc tacaagccga tcttcggcga tgacgtccgc ttttcccggg
     5581 aaagcgggaa gaggttcctg gaagaggcgc tcaaggacac cgccgtctcc gcggtggtcg
     5641 tcttcgggtc ggacgtcaat ttcctcgctt acgagccggc cttccgggag gcggggaaaa
     5701 agatggtctt cgagggtgcg gggcaggatc cgttcatcgt cttccccgat gccgacctcg
     5761 atctcgccct ctccgatctg gtcacggcga aattcatgta ttccggccag acctgcaccg
     5821 ccccgaagcg gatcttcatc caccagtcga tctacgggga attcctggag cggttcgttg
     5881 aacggaccgt caagcccgtg gtcggccccc cggaagaccc gcggacggag atctcgccgg
     5941 tggcgagccg tctggccgtc gcccggatcc gggggcaact caaggaagcg gtggcgatgg
     6001 gggcaaaggt cgtctgcggc ggaatgatcg acggaaacct gatccacccc accgtcgtgc
     6061 gggacgcaac ggacgccatg tccggcatgc gcgaggaggt cttcggcccc gtggctttca
     6121 ccacgccctt cgacacgaag gaggaagtcg tccggcgggc gaagggccac aagtatggtc
     6181 tgcgggccgc ccttttcggg ggaaaggagg cggaagaggc ggccggagag ctcgcgggcg
     6241 atccgtactg ccacccggtg ccggcctaca ccttcgggaa gttcggcacg gtcgtgcgca
     6301 accagacgag gaccgattcg tggaaagggg ccttcgtggt caaggcggtg ggaggctacg
     6361 gctactccgg ctggatctgg gagaccgtcg gcggcaagtt ccggatcaag caggggccga
     6421 agctcctcag tatcgagaca tcgatatagc ggatcgggtg gaccgccgag agaggccggc
     6481 ggcacatccc gatcgcgggc tgcttgatcc ccaagacctc catacccagg gatcacgcat
     6541 ttagatctat tgatttaact gcaatctcat tagttgctgc ctggcaggag atggtacgcc
     6601 aacaattctt tcgccggtcg ggaaaacctc aaagacggaa ttgacta
//
LOCUS       DASVJL010000499         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_371317, whole genome shotgun sequence.
ACCESSION   DASVJL010000499 DASVJL010000000
VERSION     DASVJL010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371317"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VF357_10310"
     CDS             <1..243
                     /locus_tag="VF357_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524587.1"
                     /translation="SWRGFRRALRREDRELFDALFAAARYHTAACTCAGRVVPFDAIL
                     MSIVIEERRLARELSRRLEELERRLASMLRECPDGR"
     gene            240..2339
                     /locus_tag="VF357_10315"
     CDS             240..2339
                     /locus_tag="VF357_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX7524588.1"
                     /translation="MNGWVFDIAGERDGMTVWFRTAAGETIALFAPFRPSFLLAGNRL
                     KEPALRAAAARWKCDLARGEGIDFLSGRAIPAWSFTVPGPALLGPSVRKAEKAFGPEA
                     LCNADIAPEQQFSCAIGLYPLSRAAVDCDGDGTIRSARVLDSPWDVDAPHPSFSIALL
                     RMEGTGHPAHRRVRPLEFIADGVTQSLLWENGADFLRELARLVERADPDLLVTEYGDD
                     WLLPRLLALAGRLRVPLPLGRPPRTPDDVFGGLRKRGTGIGPAAPGRVRGGRERSYVS
                     YGRVIFRAAPHTLAGRWHVDARNSFLFGETGLPGLVELSRLSGIPLQRVARTSPGTAI
                     SAMQVATALKGGILVPYKKREPEAFKSGVDLVVTDKGGLTYLPRPGAHENVGELDFAS
                     MYPSLMDRYNIYPETVNCACCRPGLPVPSIRAHTCLRRRGLVPDTIAPILVKRRLLKE
                     RQKAATEPEARELFRKRQTALKWLLVVSFAFLHGLTDAIWVKKEGAAEEDYRGLSERI
                     TVETGMPIALEGVYKWLAFLPSKRNRSVAVPNRFAGAFTNGEIKVRGIALRRSDTPPL
                     VVALQRELLERMAKAGGLSELRAMLPELREGVDAAVSGLRGGRIPMEGLAIARRLSKA
                     PERYVANTAAAAATRELCGRGVDLRPGSKIRYLLADRGGRAIGFLDGTETPDVPRYEA
                     MLREAADELMEIVACSR"
     gene            complement(2344..>2853)
                     /locus_tag="VF357_10320"
     CDS             complement(2344..>2853)
                     /locus_tag="VF357_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524589.1"
                     /translation="KQRFQQEMMKNPDLPAWHAQREKMAMAVGDRVFDKGFDRVFDSM
                     ITTLASLGCRVNNMERASGYLTSSLPQLPPEQQEGLRKDALAQYAQAKGYPPSVLQKG
                     PYDFDMGAMMERQGGAGLTLSMVKQNARQTKVKLRFDGIYYPRQVEELYRKVWAEIDK
                     QMFLDKALD"
BASE COUNT          396 a          974 c         1004 g          479 t
ORIGIN      
        1 tcgtggcggg gttttcgacg cgccttgcgc cgggaggacc gcgagctctt cgacgcgctg
       61 ttcgcggcgg cccgctacca caccgcggcg tgcacctgcg cggggagggt cgtccccttc
      121 gacgcgatcc tgatgagcat cgtcatcgag gagcgccggt tggcgcgcga gctgtcgagg
      181 cggctcgagg agctcgaacg gcggctggcg tcgatgctgc gggaatgccc cgacgggaga
      241 tgaacggatg ggtgttcgac atcgccgggg agcgggacgg gatgacggtc tggttccgca
      301 ccgcggccgg ggaaacgatc gcgctcttcg cgccgtttcg tccctcgttc ctcctcgcgg
      361 ggaaccgcct gaaagagcct gctctccgcg ccgccgccgc gcggtggaag tgcgaccttg
      421 cgcggggtga ggggatcgac ttcctctcgg gcagggcgat cccggcgtgg tcgttcaccg
      481 tgccgggacc ggccctcctc ggaccttcgg tccgcaaggc ggaaaaggcg ttcggcccgg
      541 aggcgctgtg caacgccgac attgcgccgg agcagcagtt ctcctgcgcg atcggcctgt
      601 accccctgtc gcgcgccgcg gtcgactgcg acggcgacgg gacgatccgc tccgcccgcg
      661 ttctcgattc gccgtgggac gtggacgcgc cgcatccctc cttttcgatc gctctcctcc
      721 ggatggaagg aacgggccac ccggcgcatc ggcgcgtccg cccgctcgag ttcatcgccg
      781 acggcgtgac gcaaagtctc ctttgggaga acggggcgga cttccttcgg gaactggcgc
      841 ggctcgtcga acgggccgac cccgacctgc tcgtcaccga gtacggcgac gactggctgc
      901 tgccgcggct tctcgcgctg gccggccggc tccgcgtgcc gcttcccctg gggcggcctc
      961 cgcggacccc cgacgacgtc ttcggcgggt tgcggaagcg gggcacgggt attgggcccg
     1021 ccgccccggg ccgcgttcga ggcggtcggg aacggtcgta tgtttcgtac ggccgggtga
     1081 tcttccgcgc ggcgccgcac accctcgccg gccggtggca cgtggacgcc cgcaactcgt
     1141 tcctcttcgg ggagaccggg ctgccgggtc tcgtcgaact ctcccgcctc tccggcatcc
     1201 ctctgcagcg cgtcgcccgg acgtcgccgg ggaccgccat ctccgcgatg caggtggcga
     1261 ccgccctcaa gggggggatc ctcgttccgt acaagaagcg ggagccggag gcgttcaaga
     1321 gcggcgtgga tctcgtcgtc accgacaagg gcgggctcac gtatcttccg cgccccgggg
     1381 cgcacgagaa cgtgggggag ctcgacttcg cgtcgatgta tccgtcgctc atggaccggt
     1441 acaacatcta ccccgagacg gtcaactgcg cctgctgccg tcccggcctc cccgtacctt
     1501 cgatccgcgc ccacacctgc ctccgccgca ggggactcgt ccccgatacg atcgccccga
     1561 tcctcgtcaa gcggcggctc ctgaaagagc ggcagaaggc ggcaacggag ccggaggcgc
     1621 gggagctctt ccggaagcgg cagacggcgc tcaaatggct cctcgtcgtc tccttcgctt
     1681 tcctccacgg gctgacggac gcgatctggg tgaaaaagga aggcgcggcg gaggaggatt
     1741 accgcgggct ctcggagcgg atcaccgtcg agacggggat gccgatcgcg ctcgaagggg
     1801 tctacaagtg gctcgccttc ctcccgtcga agcggaaccg atcggtggcg gttcccaacc
     1861 ggtttgcggg agcgttcacg aacggagaga tcaaggtccg ggggatcgcc ctgcgccgct
     1921 ccgacacccc gccgctggtc gtcgcgctcc agcgggagct cctcgagcgg atggcgaagg
     1981 ccggcggtct ctcggagctg cgagcgatgc tgccggagct gcgcgaaggt gtggacgccg
     2041 ccgtgtcggg acttcgcggc gggcggattc ccatggaggg gttggcgatc gcgcggcgac
     2101 tgtcgaaggc cccggagcgg tacgtcgcca acacggctgc cgccgcggcg acccgggagc
     2161 tgtgcggccg cggcgtggac ctgcggcccg ggtcgaagat ccgctacctt ttggcggacc
     2221 gcggcggccg cgcgattggg ttcctcgacg ggaccgagac tccggatgtg ccgcggtacg
     2281 aagcgatgct tcgcgaagcc gcggacgagc tgatggaaat cgtcgcctgc tcccggtgag
     2341 cgatcagtcc agcgccttgt cgaggaacat ctgcttgtcg atctccgccc ataccttcct
     2401 gtagagctcc tcgacctggc gcgggtagta gatgccgtcg aagcgaagct tcaccttcgt
     2461 ctgccgcgcg ttctgcttca ccatggaaag cgtcagcccc gcgccgcctt ggcgctccat
     2521 catggcgccc atgtcgaagt cgtagggtcc cttctggagc acagaaggcg ggtatccctt
     2581 cgcctgcgcg tactgcgcaa gggcatcctt gcgcagcccc tcctgctgtt cgggtggcag
     2641 ctgcgggagg gacgacgtga ggtatcccga ggcccgctcc atgttgttga cgcggcagcc
     2701 gagggaggcg agagtggtga tcatcgaatc gaacacgcgg tcgaacccct tgtcgaagac
     2761 ccggtcgccg accgccatcg ccatcttctc gcgctgcgcg tgccaggccg ggagatccgg
     2821 gttcttcatc atctcctgct ggaaccgctg ctt
//
LOCUS       DASVJL010000500         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1683210, whole genome shotgun sequence.
ACCESSION   DASVJL010000500 DASVJL010000000
VERSION     DASVJL010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683210"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..956
                     /locus_tag="VF357_10325"
     CDS             162..956
                     /locus_tag="VF357_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7524590.1"
                     /translation="MLKVNNIEVIYSDVILVLKGLSMVVPEGQIVALLGANGAGKSTT
                     LKAISGLLKSEEGEVTDGEILFGGEKINGKDPEEIVRKGIFQVMEGRKIFEDLTTEEN
                     LRCGAHTRKDQKNVKADYERVYTYFPRLKDRRKGLAGYLSGGEQQMLAIGRALMARPK
                     LMLLDEPSLGLSPLLVKEIFGIIKDINQKEKTTILLVEQNARVALSISSYGYIMENGT
                     VVLDGETSKLANNEDVKEFYLGMNEVGTRKSYRDIKHYKRRKRWLS"
     gene            966..2243
                     /locus_tag="VF357_10330"
     CDS             966..2243
                     /locus_tag="VF357_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7524591.1"
                     /translation="MIDRKRGYYDEALETMPAAKRAQAQREMLREAVLHAYEHAPAAR
                     RKMDDAGVRPGDVKEPADLRKIPVTRKADLKHLQKGDPPFGGLAAVSPRGMRRIYVSP
                     GPTFDPEGRDATHWRWEKPFIAAGFREGDIVQNTFMYHFSPAGLMFDEALQRIGCTVI
                     PAGVGNTELQAQVMKELDVTGYVGTPSFLMTILEKAKEMGYASGDGLSLRVALVTGEM
                     FPESLRARFRDEFGVGVRQCYGTADVGSLGYECHEAKGMHVPDEIFLEMIDPATGDPV
                     PPGAIGEVVVTLPNRTYPLVRFATGDLSILSEDPCPCGRTSPRLLKLVGRVDQVTKIK
                     GMFVHPEQVTQLAGKVAEIASAQFVVTRTDHDDHMEMRIVLKNPADASDALIARIIET
                     ARAITRLRGEVRFIATKEMEEPEKKIIDKRKWD"
BASE COUNT          506 a          755 c          794 g          377 t
ORIGIN      
        1 taaacatcat ggagtttcag aaagggaagc ccgtgatcat gaagaccatg acgcttccgc
       61 gcaaggcgga gtggctgggt ctctaatacg gtaacgcgtt tgcggacggg gggcgttcgc
      121 cccccgtccg gcttcttcca atccagggcg ggcgggaacc gatgctcaag gtgaacaaca
      181 tcgaggtgat ctactcggac gtcatcctcg tcctcaaggg gctctccatg gtcgtcccgg
      241 agggacagat cgtcgcgctg ctgggcgcca acggggcggg gaagagcacc accctcaagg
      301 cgatctcggg actcctcaag tccgaggagg gggaggtcac cgacggggag atcctcttcg
      361 gcggcgaaaa gatcaacggg aaggaccccg aagagatcgt ccggaagggg atcttccagg
      421 tgatggaggg acgcaagatc ttcgaggacc tgacgaccga ggaaaacctg cgatgcggcg
      481 cccacacccg gaaggaccag aagaacgtca aggcggacta cgagcgggtc tacacctact
      541 tcccgcgcct gaaagatcgc cggaaggggc ttgccggcta cctgtccggc ggggagcagc
      601 agatgctggc gatcgggcgg gcgctgatgg cgcgtcccaa gctcatgctc ctcgacgagc
      661 cgtccctcgg gctgtcgcca ttgctggtca aggagatctt cggcatcatc aaggacatca
      721 accagaaaga gaaaacgacg atcctcctgg tcgagcagaa cgcccgcgtg gctctttcca
      781 tctccagcta cggctacatc atggaaaacg gcacggtggt gctcgacggc gaaacttcga
      841 aactcgcgaa caacgaagac gtgaaggagt tctacctcgg catgaacgaa gtcggcaccc
      901 ggaagtcgta ccgcgacatc aagcattaca agcgccgcaa acgctggctc tcctgaggtg
      961 acgggatgat cgaccggaag aggggatatt acgacgaggc gctcgagacg atgccggcgg
     1021 cgaagcgggc ccaggcgcag cgcgagatgc tgcgcgaggc ggtgctccac gcctacgagc
     1081 acgcgccggc ggcccggcga aagatggacg acgcgggtgt tcgccccggc gatgtgaagg
     1141 agcctgccga cctgcgcaag atcccggtta cccggaaggc ggacctcaag catctccaga
     1201 agggggatcc gccgttcgga gggctggccg ccgtgtcgcc ccgggggatg cgccgcatct
     1261 acgtctcccc gggaccgacg ttcgacccgg aaggccgcga cgcgacccac tggcgctggg
     1321 agaagccgtt catcgccgcg ggattccgcg agggggacat cgtccagaac accttcatgt
     1381 accacttttc gccggccggg ctgatgttcg acgaggcgct ccagcggatc ggctgcaccg
     1441 tcatccctgc aggagttggg aacacggaac tgcaggccca ggtgatgaag gaactggacg
     1501 tcaccggcta cgtcgggacg ccgtcgttcc tcatgaccat cctcgaaaag gcgaaggaga
     1561 tggggtacgc ctccggcgac ggcctgtcgc tccgggtcgc cctcgttacc ggcgagatgt
     1621 tccccgagtc gctacgggcc aggttccgcg acgagttcgg cgtgggggtc cggcagtgct
     1681 acggcacggc cgacgtcgga tcgctgggct acgaatgcca tgaagcgaaa gggatgcacg
     1741 tccccgacga gatcttcctc gagatgatcg atcccgccac cggcgatccc gtcccgcccg
     1801 gcgccatcgg cgaagtggtg gtgacgctgc ccaaccggac gtatccgctc gtacggttcg
     1861 ccaccgggga tctctccatc ctatcggaag acccgtgccc ctgcgggcgc acctcccccc
     1921 ggctgttgaa gctggtgggg cgcgtcgatc aggtcacgaa gatcaagggg atgttcgttc
     1981 accccgagca ggtcacccag ttggcgggga aggtcgccga gatcgcctcc gcccagttcg
     2041 tcgtaacccg gaccgatcac gacgaccata tggagatgcg gatcgtcctt aaaaatcccg
     2101 ccgacgcgtc ggacgcgctc attgcccgca tcatcgagac ggcccgcgcg atcacccgcc
     2161 ttcggggcga ggtccggttc atcgccacca aagagatgga ggagccggag aagaagatca
     2221 tcgacaaaag gaagtgggac tgacagcata cgaacccggg agggatccat ggacgccgcc
     2281 acgctgaaca agcatcttga aacacacctt cgggtgcaca ccttccccct cggggtcaag
     2341 tcgctgaaac ccggggagcc gcttcccgac aaggtaaaaa ttcccttgaa gcacctgggc
     2401 gtgaaggtgg cgatctgcca agccatctcg at
//
LOCUS       DASVJL010000501         1992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1403501, whole genome shotgun sequence.
ACCESSION   DASVJL010000501 DASVJL010000000
VERSION     DASVJL010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403501"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1086
                     /locus_tag="VF357_10335"
     CDS             181..1086
                     /locus_tag="VF357_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX7524592.1"
                     /translation="MENEAERSFGFLHINERGGKPRRTGVTEIRGPYYTPLGKRALSD
                     ILETMGAYVDVLKFAGGSFVLMPESAVRELIDTCHSHDVLVSTGGFIEHVLTMGPEAV
                     DGYLEECKRLGFDVVEVSSGFISVPDDDLVRLVEKVRTLGMLPKPEVGIQFGAGGASS
                     VAELDAEGTSDPSRAIRQAKRYLEAGAHLIMIESEGITEQVKSWRTDVPARFINELGL
                     DKVMFEAADPEVFSWYVKSYGPEVNLFVDHSQVVQLECLRSGIWGTKSTWGRVVTYKR
                     SGKAVPAEMSPVRHIASHPKVRGGR"
     gene            1083..>1992
                     /locus_tag="VF357_10340"
     CDS             1083..>1992
                     /locus_tag="VF357_10340"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX7524593.1"
                     /translation="MTPYHDGAAARRLDEPRTQAERLASFVSRANWGEISPSAREALK
                     IGVLDSLGCAYGAIAGEPVRLLRKQVDEFGGKPLCTLIGGGRSAPDRAAFYNGALVRY
                     LDYNDSYLAKGETCHPSDSLGAVLAAAEYAGIPGKEFLAALAVAYQVQCRLSDVAPVR
                     ARGFDHVTHGAIAAAAGAAKALRLDPPRIANAVAIAGTANNALRVTRTGELSHWKGLA
                     APNAAAAGLQAAFLAMRGVTGPREMFEGVKGWMQVVSGPFEIDWEGENLERVNRTILK
                     KYNAEIHSQSAIEGIIGMMAEVDFPAA"
BASE COUNT          372 a          599 c          692 g          329 t
ORIGIN      
        1 ttactccggc gggccgcctc acgggtcagc ttcgggaact acaccaacgt cttctacttc
       61 ggctagtcca tccgcgctgt cgctccggga agtttggccc tggcgaaacg acatgttgtg
      121 ccgaattcat cttatttaaa gcgggacgag aagaaaccgg cttgagaagg aggaatttcc
      181 atggagaacg aggccgaaag gagtttcggt tttcttcaca taaatgagcg gggcggcaaa
      241 ccgcgtcgta ccggcgtcac ggagatccgg ggaccgtact acacccccct gggaaagcgg
      301 gcgctgtcgg acatcctcga aacgatgggg gcctacgtcg acgtgctcaa attcgccggg
      361 ggctccttcg tcctgatgcc ggagagcgcc gtgcgcgagc tgatcgacac ctgtcattcg
      421 cacgacgtcc tggtgtccac cggggggttc atcgagcacg tacttacgat ggggcccgaa
      481 gccgtggacg ggtacctcga ggagtgcaaa cgactggggt tcgacgtcgt ggaagtgtcc
      541 agcgggttca tctcggtccc cgacgacgac ctcgtccgcc tcgtggaaaa ggtgcgcacc
      601 ctgggcatgc tcccgaagcc cgaggtcggt atccagttcg gcgccggagg agccagctcg
      661 gtggcggaac tggacgcgga ggggacctcc gacccgtccc gcgcgatccg tcaggcgaaa
      721 cggtacctgg aggcgggagc gcatctcatc atgatcgaat cggagggaat cacggagcag
      781 gtcaagtcgt ggcgtacgga cgtgccggcc cggttcatca acgagctcgg cttggacaag
      841 gtgatgttcg aggcggccga tccggaggtg ttctcctggt acgtgaaatc gtatggtccg
      901 gaggtcaacc tcttcgtgga ccactcccag gtcgtccagc tcgaatgcct gcgatcgggg
      961 atctggggga cgaaaagcac ctggggccgc gtggtgacgt acaagagaag cgggaaagcc
     1021 gtacccgccg agatgtctcc cgtacggcac atcgcgagcc acccgaaggt caggggcgga
     1081 agatgacacc gtatcatgat ggcgcggctg cccggcgtct cgacgaaccc cggacgcagg
     1141 cggaacgatt ggcgtcgttc gtatcccggg cgaactgggg agagatctca ccatctgccc
     1201 gggaggcgtt gaagataggc gtcctcgatt ccctcggctg cgcgtacggg gcgatcgcgg
     1261 gggaacccgt gcgtctgctg cggaaacagg tggacgagtt cgggggaaag ccgctatgca
     1321 ccctgatcgg aggcgggcgt tccgcgccgg accgggccgc cttctacaac ggggcgttgg
     1381 tcaggtacct ggactacaac gacagctacc tggcgaaggg ggaaacgtgc caccccagcg
     1441 acagcctcgg tgcggtgctg gcagcggcgg aatacgccgg gatcccgggg aaggagttcc
     1501 tcgccgcgct ggcggtggcg taccaggtcc agtgtcgcct ctctgatgtg gccccggtcc
     1561 gggcgcgcgg attcgaccac gtgacccacg gggcgatcgc cgccgccgcg ggcgcggcga
     1621 aagcgctgcg cctcgatccc cctcggatcg cgaacgccgt ggccatcgct ggcacggcga
     1681 acaacgcgct gcgggtcacc cgtacgggag agctgtcgca ctggaagggg ctagccgccc
     1741 cgaacgccgc ggccgccggc ctgcaggccg ccttccttgc catgcggggc gtcaccggcc
     1801 cgcgggagat gttcgagggc gtcaaggggt ggatgcaagt cgtttcaggc cccttcgaga
     1861 tcgattggga aggggaaaac ctggagcgcg tcaaccggac catcctgaaa aaatacaacg
     1921 cggaaatcca ttcccagtcc gcgatagagg gaatcatcgg gatgatggcg gaggtggact
     1981 ttcctgccgc cg
//
LOCUS       DASVJL010000502         4714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1029345, whole genome shotgun sequence.
ACCESSION   DASVJL010000502 DASVJL010000000
VERSION     DASVJL010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029345"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1574
                     /locus_tag="VF357_10345"
     CDS             216..1574
                     /locus_tag="VF357_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEX7524594.1"
                     /translation="MQIEKGIDKLVKTVRMMILDKPGHFGKVATAVGNAGGSLGDIKL
                     VGYGLEYNTRDVTIFLDSEAQLQAVLEEVGKVEGVIISDIIDPVLELHRGGKIVVKSR
                     YPVDSISTVRKIYTPGVAKVCTLIQRNPELVYDYTAIFNTVAIVTNGTAILGLGDIGA
                     VAGMPVMEGKAALFSALVGINGVPILIQSKDTEEIIRTVASIAPTFGAIKLEDIKSPE
                     CFEIEDRLSAMLDIPVLHDDQHGTSVVVLAALLNAGQYVGMQVKDDTVGMVGLGAAGI
                     GISKLLMAYGVRKMLGTDINTTAQAIFAKVGGKPVSLAEVMASSDIVIATTGVPGLIK
                     KEMIRKGQVILALSNPNPEISTEDARAGGASFAADGRGVNNALAFPGVFRGALNARAR
                     KINNRMKIAAAKAIGAAASAGELVPSILDLEMHAKVAEAVERAAFESGVARPRTEEIK
                     ET"
     gene            1583..2203
                     /gene="plsY"
                     /locus_tag="VF357_10350"
     CDS             1583..2203
                     /gene="plsY"
                     /locus_tag="VF357_10350"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX7524595.1"
                     /translation="MDASWLRGTLLVLFGYLLGSVPFGILVAKAFDRDLDLRKAGSGN
                     IGATNVARILGKGAGILTLVLDAGKGVLALALARPLLDPSAYHWLALVGGAVFLGHIF
                     PVYLRFKGGKGVAVALGVVLFLSPETAFVIVVLFAAVLYFTRYVSLASLCAAVGLPVA
                     MAFLGKSRHYVTLALMMSFLVIYTHRENIHRLLSGQESRFGPPPGE"
     gene            2410..2748
                     /locus_tag="VF357_10355"
     CDS             2410..2748
                     /locus_tag="VF357_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7524596.1"
                     /translation="MKKIEAIIKPFKLDEVKESLNDIGVQGMTVSEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKIKLEIIVPDNLVAQVVELVEKSARTGRIGDGKIFVTNVEDVVRI
                     RTGERGHDAI"
     gene            2802..4214
                     /gene="glnA"
                     /locus_tag="VF357_10360"
     CDS             2802..4214
                     /gene="glnA"
                     /locus_tag="VF357_10360"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX7524597.1"
                     /translation="MNAKEVLGFAKSKNIEMVDLKFMDFIGTWQHFAVPIYELKEDSF
                     EEGFGFDGSSIRGWQPIHASDMLVIPDPETAVIDPFITRPTLSLICNIVDPITKENYS
                     RDPRNIARKAEAYLKSTGIADTAFFGPEAEFFIFDDIRYGGGANSGFFYIDSDEGTWN
                     TGREEKPNLGYKPRHKEGYFPVPPTDSQHDLRDEMVRVMEQVGLKIEAQHHEVATAGQ
                     AEIDLRFDTLVKVADALQWYKYICKNVARKAGKTVTFMPKPLYGDNGSGMHTHQSLWK
                     GGKPLFAGEEYGGMSKMALWYIGGILKHARALCAFSNPTMNSYKRLVPGFEAPVNLAY
                     SSRNRSAAVRIPMYSASPKTKRLEFRTPDPSCNGYLSFAAQLMAGLDGVQNKIDPGQP
                     LDKDIYALSPEELANVPTTPGSLEESLKALEDDHEFLLKGDVFTIDVIEKWIEYKVEA
                     EVNPVKLRPHPHEFFLYFDC"
     gene            complement(4271..>4714)
                     /locus_tag="VF357_10365"
     CDS             complement(4271..>4714)
                     /locus_tag="VF357_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524598.1"
                     /translation="TRTGAIRFEFSWNNPAAFLGLWWYLYRKMYWWALVDFALSVLLG
                     WTLFVPILWGVARAVTADYLYFLQADRRIREARPISTAFGAPATDAVHLARLAAEGGV
                     NAWVTWVAIAGTFLFFAILIFAFGWLWNAIPPLRELWQAPPGSWM"
BASE COUNT          888 a         1536 c         1537 g          753 t
ORIGIN      
        1 ggcaggtttc gccgggagtt tcgccccgtc cggcgtcgat cgccagcgcg cggccgcact
       61 tccagcatcg cgggggagcg tcccgcgcgg caaggcgctc ccgggagatg aaattctgag
      121 cggagcaggc gccgcaatcg acgatcatcg ggggtgattg tacctcccgg cgctctcgtt
      181 tgacaaaaaa gaaaaccgga cattataatg ccgccatgca gatagaaaag ggaatcgaca
      241 agctcgtaaa aaccgttcgc atgatgatcc tcgacaagcc ggggcacttc ggcaaggtgg
      301 caaccgccgt cggcaatgcg ggcgggtccc tcggggacat caagctcgtc gggtacggcc
      361 tcgaatacaa cacccgcgac gtcacgatct tcctcgacag cgaggcgcag ctccaggcgg
      421 tcctcgagga ggtcgggaag gtcgagggcg tcatcatctc cgacatcatc gacccggtgc
      481 tcgagttgca ccgcggaggc aagatcgtcg tcaaatccag gtacccggtc gacagcatct
      541 ccaccgtccg gaagatctac acccccggcg tcgccaaggt ctgcacgctc atccagcgga
      601 atccggagct ggtgtacgac tacaccgcca tcttcaacac ggtggcgatc gtcaccaacg
      661 ggacggccat cctcgggctg ggagacatcg gcgcggtggc cggcatgccg gtgatggagg
      721 ggaaggccgc gctgttttcc gcgctggtgg ggatcaacgg cgtcccgatt ctcatccagt
      781 cgaaggacac cgaggagatc atccgcacgg tggcgtcgat cgcgcctaca ttcggcgcga
      841 tcaagctcga ggacatcaag tctcccgaat gtttcgagat cgaggaccgc ctgagcgcga
      901 tgctcgacat cccggtcctg cacgacgacc agcacggaac ctccgtcgtc gtcctggcgg
      961 cgctgctgaa cgccggccag tacgtcggga tgcaggtcaa ggacgacacg gtcgggatgg
     1021 tggggctggg ggccgccggg atcggcatct ccaagctgct gatggcctac ggcgtccgca
     1081 agatgctcgg gaccgacatc aataccaccg cgcaggcgat cttcgcaaag gtgggcggca
     1141 agcccgtctc gctcgccgag gtcatggcgt cgtccgacat cgtcatcgcc acgaccggcg
     1201 tgccggggct catcaagaag gagatgatcc gcaaggggca ggtgatcctc gcgctgtcga
     1261 acccgaatcc cgagatctct actgaggacg cccgggcggg gggggcctcg ttcgcggcgg
     1321 acggccgggg ggtcaacaac gcgctggcgt ttcccggagt gttccgtggg gcgctcaacg
     1381 cgcgggcgcg caaaatcaac aaccggatga agatcgctgc cgccaaggcc atcggcgcgg
     1441 ccgcatccgc cggggagctc gtcccatcga tcctcgacct cgagatgcac gccaaggtgg
     1501 ccgaggcggt ggagcgcgcc gcgttcgaga gtggcgtcgc ccggccccga acggaagaga
     1561 tcaaagaaac gtaatccacg ccatggacgc ttcatggctc cgcggaaccc tcctcgtcct
     1621 cttcggctac ctgctcggct ccgtcccgtt cggcatcctc gtcgcgaagg cgttcgaccg
     1681 cgacctggat ctgcggaagg ccggttcggg gaacatcggc gcgacgaacg tcgcgcgcat
     1741 cctcggaaag ggcgcgggga tcctcaccct cgtcctcgac gccggaaaag gggtcctcgc
     1801 cctcgcgctc gcccgaccgc tcctggatcc gtccgcctac cactggcttg ccctggtcgg
     1861 cggggcggtg tttctggggc acatcttccc ggtctatctc cgcttcaagg ggggcaaggg
     1921 cgtcgcggtc gccctgggcg tcgtgctgtt cctgtcgccg gagacggcct tcgtcatcgt
     1981 ggtcctcttc gccgccgtgc tgtacttcac gcgttacgtg tcgctcgcgt cgctgtgcgc
     2041 cgccgtgggc cttcccgtcg ccatggcgtt cctcggaaag tcgcggcatt acgtcaccct
     2101 cgccctcatg atgtcgttcc tcgtgatcta cacccaccgg gagaacatcc accgcctcct
     2161 ctccggccag gagagcaggt tcgggcctcc gcccggggag tgaacgtgca caggccacgg
     2221 gcatcgggtg cccacggatt gtgcaaactt ccgcctgggc gttcatcgcg tcccgctcct
     2281 cccgcttcgc ctcgtcccca tggatgattg attttccaag ggaaatcaat ttccgccgcc
     2341 gccgggacgg atcggatgcc gggttggtac gaccgttgct ttgatgaaag ggggaaggga
     2401 ggtgcaccta tgaaaaagat cgaggcgatc atcaagccgt tcaaactgga cgaggtgaag
     2461 gagtcgctga acgacatcgg cgtccaaggg atgacggtct ccgaggtaaa ggggttcggc
     2521 cgccagaagg ggcacaccga actgtaccgg ggggcggaat acgtcgtcga ttttctgccc
     2581 aagatcaagc ttgagatcat cgttcccgac aacctggtcg cccaggtggt cgagctcgtc
     2641 gagaagtcgg cccggacggg ccggatcggc gacgggaaga tcttcgtcac caacgtcgag
     2701 gatgtcgtcc ggatccgcac cggcgaacgc ggtcacgacg ccatctgacc ggatcgccgg
     2761 ccgcgtccca ttccaacatt tcgcccccag gagggatcgc aatgaacgcg aaggaagtcc
     2821 tcggtttcgc caagagcaag aacatcgaga tggtcgacct gaagttcatg gatttcatcg
     2881 gcacctggca gcacttcgcg gtgccgatct atgagctcaa ggaggacagc ttcgaggaag
     2941 ggttcggctt cgacggatcg tccatccgcg gctggcagcc gatccacgcc tccgacatgc
     3001 tggtgatccc cgaccccgag acggcggtca tcgatccgtt catcacccgg ccgacgctgt
     3061 cgctgatctg caacatcgtc gacccgatca ccaaggaaaa ctactcccgc gacccccgca
     3121 acatcgcgcg gaaggccgag gcatacctga agtcgaccgg catcgccgac acggcgttct
     3181 tcggacccga ggccgagttc ttcatcttcg acgacatccg ctacgggggc ggggcgaact
     3241 ccggtttctt ctacatcgac tccgacgagg ggacctggaa caccgggcgc gaggagaagc
     3301 caaacctcgg ctacaagccg cgccacaagg aagggtactt ccccgtgcca ccgaccgact
     3361 cgcagcacga cctgcgcgac gagatggtcc gcgtcatgga gcaggtgggg ctgaagatcg
     3421 aggcgcagca ccacgaggtg gccaccgccg gccaggccga gatcgacctc cggttcgaca
     3481 cgctggtgaa ggtcgccgac gcgctgcagt ggtacaagta catctgcaag aacgtcgcgc
     3541 ggaaggccgg caagaccgtc accttcatgc cgaagccgct ctacggcgac aacggatccg
     3601 ggatgcacac gcaccagtcc ctctggaagg gcggcaagcc gctcttcgcc ggggaggagt
     3661 acggcgggat gtcgaagatg gcgctctggt acatcggcgg gatcctgaag cacgccaggg
     3721 ctctctgcgc gttcagcaac ccgacgatga actcctacaa gcggctggtg ccgggcttcg
     3781 aggcccccgt gaacctcgcg tactcgagcc ggaaccgctc cgcggcggtc cggatcccga
     3841 tgtactccgc ctcgccgaag acgaagcgcc tcgagttccg cacgcccgac ccgtcctgca
     3901 acggctacct ttcgttcgcc gcgcagctga tggcgggtct ggacggcgtc cagaacaaga
     3961 tcgaccccgg gcagccgctc gacaaggaca tctacgcgct gtcgcccgag gagctggcga
     4021 atgtgccgac cacgccgggc tccctcgagg agtcgctcaa ggcgctggag gacgaccacg
     4081 agttcctgct gaagggcgac gtgttcacca tcgacgtgat cgagaagtgg atcgagtaca
     4141 aggtcgaggc ggaggtgaac cccgtgaagt tgcggccgca cccgcacgag ttcttcctgt
     4201 acttcgactg ctgaaccggc cgaccgaagg aacgctccgc aaaggggccc cggcgacggg
     4261 gcccttttgc ttacatccag ctgcccggag gagcctgcca gagttcgcgc agcggcggga
     4321 tcgcgttcca gagccacccg aaggcgaaga tgaggatggc gaaaaaaagg aaggtccccg
     4381 cgatggcgac ccacgtgacc caggcgttga cgccgccctc ggcggcgagc cgggcgaggt
     4441 gcaccgcgtc cgtggcgggc gcgccgaagg cggtggagat cgggcgggcc tcccggatcc
     4501 tccggtcggc ctgcaggaag tagaggtagt cggccgtgac cgcgcgggcg acgccccaca
     4561 ggatcgggac gaagagggtc cagccgagca ggacgctcag agcgaagtcg accagcgccc
     4621 accagtacat cttccggtag aggtaccacc acagccccag gaaggcggcc ggattgttcc
     4681 aggagaactc gaagcggatc gcgcccgtcc gggt
//
LOCUS       DASVJL010000503         4195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1593348, whole genome shotgun sequence.
ACCESSION   DASVJL010000503 DASVJL010000000
VERSION     DASVJL010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593348"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF357_10370"
     CDS             complement(<1..228)
                     /locus_tag="VF357_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524599.1"
                     /translation="MNGSDHLSRTDPGALYRELSRSNLPATRRWYAVTGLLLLVTLWG
                     VGCFVYMMMTGMGVTGLNRPVMWGALIASFVF"
     gene            complement(225..923)
                     /locus_tag="VF357_10375"
     CDS             complement(225..923)
                     /locus_tag="VF357_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7524600.1"
                     /translation="MNIKWAMAIDLDRCTGCGACVVACVAENNIPVVGEAEVEKGRLM
                     QWLRVARYWEGEYPEAQAVYLPVPCMQCERAPCETVCPVYATYHNEDGLNAMVYNRCV
                     GTRYCANNCPYSVRAFNWFDPKWEKPLDEQLSPDLTTRSRGVMEKCTFCIQRIRRAKE
                     TAAQEGREIRDGEVLPACVQTCPPGALLFGDRADPHSEITRKLKDPRRFRLMEHLGTE
                     PRVVYLKRQKGAGR"
     gene            complement(979..3150)
                     /locus_tag="VF357_10380"
     CDS             complement(979..3150)
                     /locus_tag="VF357_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7524601.1"
                     /translation="MLDRRQFLKLGGATAFATLFGGCDKVQRTLIPFVIPPENYTLGE
                     ALWYASVCGQCPAGCGIVVRVSEGRAKKIEGNPLHPVNRGKLCARGQATLQALYHPER
                     LSRPMKLSGARGSGSYTPVSWEEALSLLMGPLKKAKAEAPGSLLMLTGPMRGHAGLVA
                     GRFMKAFGSKHRVSWDPFGPDAMLSANAAVHGVRDLPEHDLANARYLLSFSGDFLETG
                     ISPVHYSGAYGRMRGDRETIRGKVVHFGPRLSMTAAAADLFLPCAPGTEGFVALGIAH
                     VMVRDRLTAASSAAGRLADDLGAYSPEEVEKKTGVHAHDIAAVAEEFSRNQPGLAIAG
                     TGAATGPDGAFHCAAAALLNVLAGNVGNPGGVSFPNRAQAFAKYGAEAALLAPSPESG
                     YPGMRAELEKMRGGAFRMAILAGVTNPAFTLPPPLKFNDALSKVPFVVAFASFLDETS
                     SLADLVLPVPTALEAWGDDLAPVGHSGAVTLCQPVVDPFHDTRSMPDVLIAAAKELGG
                     EPAKVLPWGSFRECIEKAYGGPGGEMEKALQRGGFFGESSSPRSAVRTGNVALPKPTE
                     RPIDGDPKAFPLALHLYPSITLYDGRGANLPWLQEMPDPITTAVWRNWVEVNPKTAAG
                     LGLADGDGVNVTSPSGKITAHVAINPGIASDVAAMPLGQGHTRYGKYATGRGENPFAL
                     LPAGETYALQATRGALTKTKLSARLVRFAHPEGQWKISNLM"
     gene            complement(3172..3675)
                     /locus_tag="VF357_10385"
     CDS             complement(3172..3675)
                     /locus_tag="VF357_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX7524602.1"
                     /translation="MTRKRTLATLAIALLPLALLLATGYAFAAETIPQPIQFSHKIHA
                     TDYKMNCRFCHSSTNKSQYANLPSVEKCMICHRSIATDKPEVKKVAQYWNEKKPIPWN
                     KVIDLPNHVYFPHKKMVNAGIACLTCHPGMDQAGTAVQKLEFGMGFCMDCHRKKKVSI
                     DCWTCHY"
     gene            complement(3763..>4195)
                     /locus_tag="VF357_10390"
     CDS             complement(3763..>4195)
                     /locus_tag="VF357_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524603.1"
                     /translation="AFREWLPLSPPRPGAIGLAALLLSCAGLFAVGIVPDFVFPLVWV
                     APPTLLISLAALRGEPHSLSGIASGDWRPAVSSVLAALLCGFFWETWNFGSYAKWVYA
                     IPYVDALHVFEMPLLGYAGYLPFGVLCAEIGFLIIGSVRKE"
BASE COUNT          687 a         1378 c         1436 g          694 t
ORIGIN      
        1 gaagacgaac gaggcgatca gggcccccca catgaccggc cggttgagcc ccgtgacccc
       61 catcccggtc atcatcatgt agacgaagca gcccactccc caaagggtga ccagaagaag
      121 gaggcctgtg accgcgtacc aacgcctcgt cgcggggaga ttggaccggg agagctcgcg
      181 atagagcgcg cccggatcgg tccgggacag atggtcgcta ccgttcatcg accggcccct
      241 ttctggcgct tgaggtacac caccctcggc tcggtcccga ggtgctccat caggcggaac
      301 cgtcgaggat ccttgagttt ccgggtgatc tcgctgtgcg gatcggcccg gtccccgaag
      361 aggagcgctc ccggcgggca cgtctggaca cacgcgggaa ggacctcccc gtcgcggatc
      421 tcccgtcctt cctgcgccgc ggtctccttc gcccgccgga tccgctggat gcagaaggtg
      481 cacttctcca tgacgccgcg ggagcgcgtg gtgaggtcgg gggagagctg ctcgtcgagc
      541 ggcttttccc acttgggatc gaaccagttg aaggcccgca cggagtaggg gcagttgttc
      601 gcgcagtaac gagtgccgac gcaacggtta tagaccatcg cgttcaggcc gtcctcgttg
      661 tggtaggtcg cgtagaccgg gcagacggtc tcgcacgggg cgcgttcgca ctgcatgcac
      721 ggcacgggga gatagaccgc ctgcgcctcc gggtattccc cctcccagta ccgggcgacc
      781 cggagccatt gcatcaagcg ccctttttcc acctcggcct cgccgaccac cgggatgttg
      841 ttctccgcga cgcaggcgac gacgcacgcg ccgcatccgg tgcaccggtc caggtcgatc
      901 gccatcgccc acttgatatt catcgcttct cccgactctc attcgccgaa cagggacgtt
      961 ccagggaact cccgtcgatc acatcaggtt gctgatcttc cattgcccct cagggtgcgc
     1021 gaaccggacc agccgggcgg aaagtttcgt cttcgtcagg gcgccccgtg tcgcctggag
     1081 cgcgtacgtt tcgcctgcag gcaggagggc gaacgggttc tcccccctcc cggttgcgta
     1141 cttgccgtac cgcgtgtgtc cctgccccag cggcatcgcc gcgacgtccg atgcgatgcc
     1201 cgggttgatg gccacgtggg ccgtgatctt ccccgaaggt gaggtgacgt tcacgccgtc
     1261 cccgtcagcg aggcccagcc ccgccgccgt cttcgggttc acctcgaccc agttccgcca
     1321 caccgcggtc gtgatcgggt ccggcatctc ctggagccac gggaggttcg ccccgcgccc
     1381 gtcgtagagg gtgatggagg ggtagaggtg cagcgccagg gggaacgctt tcgggtcgcc
     1441 gtcgatgggg cgctccgtcg gtttcgggag ggcgacgttc cccgttcgga ccgccgaccg
     1501 gggggaggac gactccccga agaagccgcc gcgttggagc gccttctcca tctctccgcc
     1561 gggacctccg tacgctttct cgatgcactc ccggaaggag ccccacggca gcaccttcgc
     1621 cggttctccg cccagttcct tcgccgcggc gatcaggacg tcgggcatcg accgggtgtc
     1681 gtggaacggg tccaccaccg gctggcagag ggtgaccgcc ccggaatgcc ccacgggagc
     1741 gagatcgtcc ccccacgcct cgagggcggt cgggacggga agcacgaggt cggccagcga
     1801 cgatgtttcg tcgaggaagg aggcgaaggc gacgacgaag gggaccttcg acagcgcatc
     1861 gttgaatttg agcggcggcg ggagggtgaa cgcagggttc gtcacccccg cgaggatggc
     1921 catccggaac gcgccgccgc gcattttttc gagttcggcg cgcattcccg ggtacccgct
     1981 ttccggcgag ggggcgagca gggcggcttc cgccccgtac ttcgcgaacg cctgcgcgcg
     2041 gttcgggaac gataccccgc cggggtttcc cacattcccc gcgagcacgt tcaggagcgc
     2101 cgcggcggcg cagtggaacg ccccgtcggg accggttgcg gcccccgtcc cagcgatcgc
     2161 caggccgggc tggttgcggg agaactcctc ggcgacggcg gcgatgtcgt gggcgtggac
     2221 cccggtcttc ttctcgacct cctcggggga gtaggcgccc aggtcgtccg caaggcgccc
     2281 ggcggcggaa gacgcggcgg tgagccggtc ccgcaccatc acgtgcgcga tcccgagggc
     2341 cacgaaccct tccgtgccgg gggcgcacgg aaggaacagg tcggcggcgg cggcggtcat
     2401 cgacagccgg gggccgaagt ggacgacctt cccgcggatc gtctcgcggt cgccccgcat
     2461 ccggccgtac gcccccgaat agtggacggg agagatgccg gtctcgagaa agtcgccgga
     2521 gaaggagagc aggtaccgcg cattcgcgag gtcgtgctcc ggcaggtcac ggacgccgtg
     2581 gaccgccgcg ttcgcggaca gcatcgcgtc ggggccgaac gggtcccagg agacacgatg
     2641 ctttgatccg aacgccttca tgaaccggcc ggcgaccagc cccgcgtggc cgcgcatcgg
     2701 cccggtgagc atcaggagcg accctggcgc ctcggccttc gctttcttca acggccccat
     2761 caggagggag agggcctctt cccaggagac cggcgtgtac gaccccgacc cgcgcgcgcc
     2821 ggagagcttc atcggccgcg agagccgctc cgggtggtag agcgcctgca gcgtcgcctg
     2881 tccacgggcg catagtttcc cccggttcac cgggtggagc gggttcccct cgatcttctt
     2941 cgcccgccct tccgagacgc ggacgacgat cccgcacccg gcggggcact ggccgcacac
     3001 cgaggcgtac cagagcgcct cgcccaaagt atagttctcc ggggggatga cgaacgggat
     3061 cagcgtgcgc tgcaccttgt cgcacccgcc gaagagggtg gcgaaggccg tcgccccgcc
     3121 gagtttcagg aattgtcgcc gatcgagcat ccgtttcctc tcgcgtcccc gtcagtagtg
     3181 gcaggtccag cagtcgatgg agaccttttt cttccggtgg caatccatgc agaagcccat
     3241 gccgaattcg agcttctgga cggccgtccc cgcctgatcc atccccgggt ggcaggtgag
     3301 gcaggcgatt cccgcgttca ccatcttttt atgggggaag tagacgtgat tggggagatc
     3361 gatcaccttg ttccagggga tcggcttctt ctcgttccag tattgcgcga ccttctttac
     3421 ctcgggcttg tcggtcgcga tcgaccggtg gcaaatcatg cacttctcca ccgaggggag
     3481 gttggcgtac tgcgacttgt tcgtggagga gtggcagaag cggcagttca tcttatagtc
     3541 cgtggcgtgg attttatggc tgaactggat cggctgcggg atcgtttccg ccgcgaacgc
     3601 atacccggtg gcgaggagaa gggcaagcgg caggagcgcg atggccaacg tcgcgagagt
     3661 acgcttccgg gtcatcggac cccttccagc gaagtgggga tgaacggcgg catgcaccgc
     3721 gatccgattt tatcccaccc gttgtgaaac aggaattttt ttctattcct tgcggacgga
     3781 gccgatgatg aggaacccga tttcggcgca cagcaccccg aacggcaggt agccggcata
     3841 cccgaggaga ggcatttcga acacgtgcaa ggcgtcgacg taggggatgg cgtacaccca
     3901 cttcgcgtag cttccgaaat tccacgtttc ccagaagaag ccgcagagaa gggcggcgag
     3961 gacggaggat accgccgggc gccagtcgcc ggaggcgatc ccggaaaggg agtgcggctc
     4021 tccccggaga gcggcgaggg agatcaggag cgtgggcggg gcgacccaga ccagcgggaa
     4081 cacgaagtcg gggacgattc cgacggcgaa gagccctgcg caggagagga gcagcgcggc
     4141 gaggccgatc gcccccggtc ttggcggaga gagggggagc cattcgcgga acgcg
//
LOCUS       DASVJL010000504         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1593577, whole genome shotgun sequence.
ACCESSION   DASVJL010000504 DASVJL010000000
VERSION     DASVJL010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593577"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1549)
                     /gene="selA"
                     /locus_tag="VF357_10395"
     CDS             complement(137..1549)
                     /gene="selA"
                     /locus_tag="VF357_10395"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX7524604.1"
                     /translation="MPDSRGLRSLPSVASLLASDAARAFLRSHPRAVVVDALREVIAA
                     ARAAVGEERVPLSREVWTDRILSMLPGEIATRESLPMRRVINAAGVVVHTNLGRAPLP
                     VEALAAVLDTGRGYSNLEFDLAAGERSSRLSHVDGMLRALTGAECAHVVNNNAAAVLL
                     CLAGHARGREVIVSRGELVEIGGSFRIPDIMAESGARLVEVGTTNRTRLSDYENAVTG
                     QTALLLKVHPSNFSVHGFTEEVRTADLARLGERAGIPVMEDQGSGGLIDLAPHGIPGA
                     VSLRQALSAGPGIVTASGDKLLGGPQAGIILGKEDLVAPLKKHPLSRALRVDKMCLAA
                     LSAVLRLYADERRARDRVPVLRMLLEPEERVRARARRLVRAIRAIGTELSLAIERGGT
                     SPGGGALPDIFLPTARVAVSHPRIPEAVLEERLRRGTPRVVARVGKGKVLLDLRTVRD
                     DEVPELAAAVSAIDRPPYDG"
     gene            complement(1563..>1847)
                     /locus_tag="VF357_10400"
     CDS             complement(1563..>1847)
                     /locus_tag="VF357_10400"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX7524605.1"
                     /translation="KDLRRLLDLHNEFHDIFLRSCGNEKLHAIVQNLVMQFRRFRLIL
                     SMPGRIEGSIRQHWEIIDAFRKRDPELAEDLVRKNALYGKKLLLRELAKA"
BASE COUNT          296 a          634 c          650 g          267 t
ORIGIN      
        1 cccgggcaag ccccgagtac agcaggcaaa aggcccggcg cgcgttcgtg acgtggaccg
       61 ggaagtaccc ccggttgagg acaaggacgc tggagttcag catcggacgg cccatggttt
      121 catggtggtt cctactctac ccatcgtacg ggggccggtc aatggcagac acggccgcgg
      181 caagctccgg aacctcatcg tcccggacgg tccgcagatc gaggagcacc ttccctttcc
      241 cgacccgggc gaccacgcga ggcgtccccc ggcgcagccg ctcttccagg accgcttccg
      301 gaattcgcgg gtgcgaaacg gcgacgcggg cggtcggaag gaagatgtcg ggaagggcgc
      361 cgccgccggg ggaggtcccg ccccgctcga tggcgaggga cagttcggtc ccgatggcgc
      421 ggatcgcccg cacgaggcgg cgcgccctgg cgcgcacccg ttcctcgggt tcgaggagca
      481 tccggaggac gggaacccgg tcccgcgccc tgcgttcgtc cgcatagagg cgaaggaccg
      541 ccgatagcgc ggcgaggcac atcttgtcga cccggagcgc ccgggagagc gggtgcttct
      601 tcagcggcgc gaccaggtct tcctttccga ggatgatccc cgcctgcggt ccccccagga
      661 gcttgtcccc gctcgcggtc acgatccccg ggccggcgga cagggcctgc cgcagggaca
      721 ccgcgccggg aataccgtgc ggtgcgagat cgatcagtcc ccccgatccc tggtcttcca
      781 tgaccgggat ccccgcgcgc tcgcccaacc gggcgaggtc ggcggtgcgg acctcctcgg
      841 tgaacccgtg aacggagaag ttggacgggt ggaccttgag caggagcgcg gtctgccccg
      901 tgacggcgtt ctcgtaatcg gaaaggcgcg tccggtttgt cgtccccacc tccaccagcc
      961 gggcaccgct ctccgccatg atgtcgggga tccggaacga cccgccgatc tcgacgagct
     1021 cgccgcggct gacgatcact tcccgccccc gggcgtggcc cgcgagacag agcagcaccg
     1081 cggcggcgtt gttgttgacg acgtgggcgc actcggcgcc ggtcagggcg cgcagcatcc
     1141 cgtcgacgtg cgagaggcgg gacgatcgtt cccccgccgc gaggtcgaac tcgaggttcg
     1201 aatacccccg gccggtgtcg agaaccgcgg caagcgcctc cacgggcaac ggcgcacgcc
     1261 cgaggttggt gtggacgacg accccggcgg cgttgatcac ccggcgcatc gggagggact
     1321 ccctcgtcgc aatctctccc gggagcatcg agaggatccg atcggtccac acctcgcgcg
     1381 agagcggaac ccgttcctcg ccgacggcgg cgcgggcggc ggcgataacc tcgcggagcg
     1441 cgtccaccac cacggcacgg ggatgggacc gcaggaacgc gcgggcggcg tcgcttgcca
     1501 gcagggacgc gacggacggg agactgcgga gtcctcggga atccggcatc gccgacgccg
     1561 gatcaggcct tcgccagttc ccggagaagg agtttcttgc cgtagagggc gttcttgcgc
     1621 acgagatcct cggcgagttc cgggtcgcgc ttgcggaatg cgtcgataat ctcccaatgc
     1681 tgccggatcg acccctcgat cctccccggc atggagagga tcaggcggaa gcgccggaac
     1741 tgcatgacca ggttctgcac gatcgcgtgg agcttctcgt tcccgcacga gcgcaggaag
     1801 atgtcgtgaa actcgttgtg caggtcgagc agccggcgaa ggtcctt
//
LOCUS       DASVJL010000505         3072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_372657, whole genome shotgun sequence.
ACCESSION   DASVJL010000505 DASVJL010000000
VERSION     DASVJL010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372657"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VF357_10405"
     CDS             <1..1067
                     /locus_tag="VF357_10405"
                     /inference="COORDINATES: protein motif:HMM:NF015706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX7524606.1"
                     /translation="NSTRGTKERVGRLLRMHANKREDVKRVAAGDIAAAVGLRNAKTG
                     DTLCLEAKPIVLESIVFPEPVVSVAIEPKTKDGQERLSMALASLSNEDPTFRAMTDEE
                     MGQTIISGMGELHLEIIVDRLLREFRVEANVGKPQVAYRETVTVSVPAEGRFIRQTGG
                     RGQYGHVKIQLEPLPGGGFEFVNKVIGGTIPKEYIPAVEKGIREAMEGGVLAGYPVVD
                     TRVVLLDGSYHEVDSSEMAFKIAGSMAFKDGARRAKPTLLEPIMRFDVIMPEEFVGQV
                     IGDLNSRRGRVLEIEARPAAQVVRTEVPLAELFGYATDLRSATQGRASHTPFQFARYH
                     PVPTQIAEEIVAKVGGRAGA"
     gene            1157..2359
                     /gene="tuf"
                     /locus_tag="VF357_10410"
     CDS             1157..2359
                     /gene="tuf"
                     /locus_tag="VF357_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX7524607.1"
                     /translation="MGKAKFERTKEHMNVGTIGHIDHGKTTLTAAITKALGFKNPKIA
                     FVPFDQIDKAPEERERGITIAIAHVEYETDKRHYAHVDCPGHADYIKNMITGAAQMDG
                     AILVVAASEGPMPQTREHILLARQVGVPSMLVFLNKVDQVDDPELMELVELEVRELLT
                     QYGFPGDEIPIVKGSALKALQSTSKDPKAPEYACIYELAAAVDTYFPSPKREIDKPFL
                     MPVEDVFTISGRGTVVTGRVERGVVKVGEEVEIVGIRPTQKTVCTGVEMFRKLLDQGQ
                     AGDNIGALLRGIKREEVERGQVVAKPGSITPHTKFKAEAYILTKEEGGRHTPFFDGYR
                     PQFYFRTTDVTGVAKLPQGTEMVMPGDNVTMEVTLIQPIAMEKELRFAIREGGRTVGA
                     GVVSEVLE"
     gene            2406..2714
                     /gene="rpsJ"
                     /locus_tag="VF357_10415"
     CDS             2406..2714
                     /gene="rpsJ"
                     /locus_tag="VF357_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX7524608.1"
                     /translation="MDNQKIRIKLKAYDHRLLDQAVKDIVGTVERSGALVSGPIPLPT
                     KISRYTVLRSPHVDKKSREQFEIRTHVRMMDIVQPTPQTVDQLMRLDLPAGVDVEIKL
                     "
     gene            2783..>3072
                     /locus_tag="VF357_10420"
     CDS             2783..>3072
                     /locus_tag="VF357_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX7524609.1"
                     /translation="MAIGLLGRKVGMTQVFDAKGKAVPVTIIQAGPCPVVQKKTAVRD
                     GYEAIQVGFETQPNKNRMTRPLQGHFDKGQVAYQRFLREIRLRGGEGEYQVG"
BASE COUNT          598 a          923 c         1063 g          488 t
ORIGIN      
        1 tcaattcgac ccggggaacc aaggagcggg taggccgcct cctccggatg cacgccaaca
       61 agcgcgaaga cgtcaagcgc gttgcggcgg gtgacatcgc ggcggcggtg ggactccgga
      121 acgccaagac gggagacaca ctctgcctgg aggcgaagcc gatcgtcctg gagtcgatcg
      181 tgttccccga gccggtcgtc tcggtggcca tcgagcccaa gaccaaggac ggacaggagc
      241 ggctgtcgat ggcgctggcc tccctgtcca acgaagatcc gaccttccgg gcgatgaccg
      301 acgaggagat gggccagacc atcatctcgg gcatggggga actccacctg gagattatcg
      361 tggatcgcct tctccgggag tttcgggtcg aggccaacgt ggggaaaccc caggtggcct
      421 accgagagac ggtgacggtg tctgttccgg cggagggtcg cttcatccgg caaaccggcg
      481 gacgcgggca gtacggccat gtgaagattc agctcgaacc actgccaggc ggcgggtttg
      541 agtttgtgaa caaggtcatc ggcgggacga ttcccaaaga gtatatcccg gcggtggaga
      601 aggggatccg cgaggccatg gaggggggag ttttggcggg ctatccggtg gtggacacgc
      661 gggtggttct gctcgacggc tcctaccacg aggtggactc gtcggagatg gcgttcaaga
      721 tcgccggctc gatggcgttc aaggacgggg cgcgccgggc gaagccgacc ctcctcgaac
      781 cgatcatgcg ctttgacgtg atcatgccgg aggagttcgt ggggcaggtg atcggtgacc
      841 tgaactcccg gcggggacgg gtgttggaga tcgaggcgcg gcctgccgcg caagtggtcc
      901 ggaccgaggt gccgctcgcg gaactcttcg ggtatgccac cgatcttcgc tccgccactc
      961 agggtcgagc cagccacact ccgtttcaat tcgctcggta ccatccggtg ccgacgcaga
     1021 ttgccgaaga gatcgttgcc aaggtgggcg gccgagcggg cgcgtgaccg tgcgggcacc
     1081 cagaagggtg ccgcggtggg attgcgggcc ggtatgatgg cctccggaga ggaggcctgc
     1141 gcagggagac actctcatgg ggaaagcgaa gttcgagcgg acgaaagagc acatgaacgt
     1201 cgggaccatt ggccacatcg accatgggaa aacgacgctg accgcggcga tcacgaaggc
     1261 cctcggcttc aagaacccga agatcgcctt cgttccgttc gaccagatcg ataaggcgcc
     1321 ggaagagcgg gagcggggca ttaccatcgc catcgcccac gtggagtacg agaccgacaa
     1381 acgccactac gctcacgtgg actgccccgg ccacgccgac tacatcaaga acatgatcac
     1441 cggggcggcc cagatggacg gcgccatcct ggtggtggcg gcctccgaag gcccgatgcc
     1501 ccagacccgg gagcacatcc tcctcgcccg ccaggtcggc gtccccagca tgctggtctt
     1561 cctgaacaag gtggaccagg tggacgaccc cgagctgatg gagctggtcg agctggaagt
     1621 ccgggagttg ctcacgcagt acggcttccc cggggacgag attccgatcg tgaagggctc
     1681 ggccctgaag gccctccagt ccaccagcaa ggacccgaag gcccccgagt acgcctgcat
     1741 ctacgagctc gcggccgccg tggacaccta cttcccgtcc ccgaagcgcg agatcgacaa
     1801 gcccttcctg atgcccgtgg aggacgtctt caccatctcc gggcgcggga cggtggtcac
     1861 cggccgggtg gagcgcgggg tggtcaaggt cggcgaagag gtggagatcg tcggcatccg
     1921 gccgacccag aagacggtct gcaccggcgt ggagatgttc cggaagctcc tcgaccaggg
     1981 ccaggccggc gacaacatcg gcgcgttgct gcgcgggatc aagcgtgaag aggtggagcg
     2041 cgggcaggtc gtggccaagc cgggctccat caccccccac acgaagttca aggccgaggc
     2101 ctacattctc accaaggagg agggtggacg ccacaccccc ttcttcgacg gctatcgccc
     2161 ccagttctac ttccggacga ccgacgtcac cggggtggcc aagctccccc aggggaccga
     2221 aatggtcatg cccggcgaca acgtcaccat ggaagtcacc ctgatccagc ccatcgccat
     2281 ggagaaggag ctgcgcttcg ccatccgcga gggcggccgg accgtgggcg ccggggtggt
     2341 gagcgaggtg ctggagtaac cggtcaggtt tcggcaactg cccagagagg gcgtcaggtt
     2401 agcgtatgga caaccagaag atccgtatca agttgaaggc gtacgatcac cggttgctcg
     2461 atcaagccgt gaaggacatt gtcgggacgg tggagcgctc tggcgcgctg gtttccggtc
     2521 cgattcctct gccgacgaag atcagccggt ataccgtgct ccgctccccg cacgtggata
     2581 agaaatcgcg ggagcagttc gagattcgga cccatgtccg gatgatggac atcgtccaac
     2641 ccacgccgca gacggtggac cagcttatgc ggttggatct gccggctggc gtggacgtcg
     2701 agatcaaact ctgagcaggg tgtcggtggc ccggcccgcc cattggcgag tatcgtcggc
     2761 cgaccccata gcgtgtgtag cgatggcgat cggattgcta ggccgtaaag tcgggatgac
     2821 gcaggtcttt gacgcgaagg gcaaagccgt gccggtgacg atcatccagg ccggtccgtg
     2881 tccagtcgtg cagaagaaga cggcggttcg ggatggctac gaggcgatcc aggtcggctt
     2941 cgagacccaa ccaaacaaga accgaatgac ccgtcccctg caggggcact tcgataaagg
     3001 ccaggtcgcg tatcagcggt ttctccgaga gatccgcctg cgcgggggtg agggggaata
     3061 ccaggttggg ca
//
LOCUS       DASVJL010000506         8230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1030244, whole genome shotgun sequence.
ACCESSION   DASVJL010000506 DASVJL010000000
VERSION     DASVJL010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030244"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..879
                     /locus_tag="VF357_10425"
     CDS             118..879
                     /locus_tag="VF357_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX7524610.1"
                     /translation="MPRFDAEYVPHSAMAIYAHPDDIEFTIAGTVAKWVRAGCEVTFV
                     LITSGNSGTHDRKFTRKSLTRVREREERESARILGVARVVFLRHGDCELVPTLALRKE
                     LVRRIRRHRPEVVLCNDPQALFFGDRYINHPDHLAAGKAALEAVFPCAEMELLWPGAG
                     LPHKVHAVYVSSTSAPDTWIDVTDTIETKIAALTAHRSQLGDRDIAPDILGRAKDEAR
                     RAPKSAGKKGPGRPKHAEAFRVMRLLRDEPTKDND"
     gene            complement(1403..2023)
                     /locus_tag="VF357_10430"
     CDS             complement(1403..2023)
                     /locus_tag="VF357_10430"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX7524611.1"
                     /translation="MVTRILEALAGFVIFVISSLGLPGIVLLMAIESACIPLPSEVIM
                     PFSGYLVYKGVHSLWEVSLAGAFGCVVGSVPAYYLGMYGGRPLIEKYGKYLLMSRHDL
                     DLADRWFTRHGEATVFFARLLPVIRTFIAFPAGVARMDMKRFLLYTIAGSFPWCLGLA
                     YLGMVLGEKWPTLREYFHRFDLLIGAVLVAGVVWYVRRHLKNRVAG"
     gene            complement(2061..3455)
                     /locus_tag="VF357_10435"
     CDS             complement(2061..3455)
                     /locus_tag="VF357_10435"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX7524612.1"
                     /translation="MSENAALSELPVSRILSALLSRGGEHGELFFEEARSLTVLMEDG
                     RIERVVSGTDAGIGVRLLFRGKTYYAYTNDRSAESLLALSSDLSRYAEGDGVAVVLPA
                     SVQSIRGPTVVRVPPAARGIGEKVALVRKVDRIAREFSREVRQVRATWSESLRHIEVA
                     AHPGVFVREEQTYCVLAVQAVAGDDGGLTMGYESAGGTEGLEFLDGGVPSLLARGAAG
                     RAVRALHAAKLPGGRMPVVLSSEAGGTMVHEAVGHGLEADLARRGMSVYKEKLGERIG
                     SPLVSIADDATVPGKRGSFGIDDEGTPGQRTLLVDAGILKGFLHDRLSAMKDGAAPTG
                     NGRRESYRHKPIPRMTNTLILPGNTAPGGILSGTDRGLQVVKMGGGQVNTVTGDFMFE
                     VAEGYRIEGGKRGEPVRGATITGNGPKVLSMIDRVGSDLGFGIGTCGKDGQGVPVGDA
                     QPSLSIGPPFITVG"
     gene            3526..5226
                     /locus_tag="VF357_10440"
     CDS             3526..5226
                     /locus_tag="VF357_10440"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7524613.1"
                     /translation="MRTIIATGALALLLFLAPAGASNGVAGPIPPGPDARAYANFLAG
                     YLDSREGNLDGALGSYKKALKYAGDEPDILYEIANVLVKKGRLPEAQAELEKVLAADE
                     GHTRSRYLLAGILAASGEREKALADYGRVLKEDPENDDAYLHIATLYAERGDFSKAEE
                     VLGTLIARDPESYLAYYYRGRVLAAQKKFEEALTDYDKVLSIAPAFDAALLESGAVLE
                     ILGRNAEAEERYKKALQSSPNNAFIRDRLGSLLIREKKIDQAVGEYEELKKFSAANPD
                     VRTKLGLLYLDRDRFDDAINEFTFVLASDPGNSQVRFFLGTAYEEKGAVPEAEAAFRK
                     IPEVDPVHREAMLHLAMLLSRQKKSDEAIEVVRKLREKSPGDVELMIFLAGQLEGAKR
                     YDEALAVASEATEKAPENPAAWFAVGVVQDKLGKLDQVIAAMEKVIAIDPKHATALNY
                     LGYTFADRNMRLPEAEKLILRALEIRPDDAYFLDSLGWVHYRRGDYRRAEEELLRALK
                     LVPDDPVLLEHLGDVLQAQGRDAEAAARFEKAIAKGHEKPDEVKAKLHRLRKAGPAGK
                     "
     gene            5223..5882
                     /locus_tag="VF357_10445"
     CDS             5223..5882
                     /locus_tag="VF357_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524614.1"
                     /translation="MRFRSTKPLRAFAPLLAVVALCACAPSRMIVTGPEADHAERFFA
                     GLPGRVVFPVKASFSGTAVPVAGDAVPFVAGISAPSPEEETVGLYDPLGHGVAFLAND
                     GRRVAISRGPAADLAGFRFAAPLDAGPVSLARILSGAPGYAVASAEAARYEDGAWSLS
                     DSRQTLRSDPGRRFLAGADYRVPGMRVTVDYPGRKSADPPDRIVLSVRGVTFTLRRDA
                     E"
     gene            5879..7519
                     /locus_tag="VF357_10450"
     CDS             5879..7519
                     /locus_tag="VF357_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEX7524615.1"
                     /translation="MSLLPRRAVLPSIALFLLFAACGGGKPESAGKGAAVSDVPAYGD
                     AIVEGSIGDVSGFLTAVTTDASSHEAAGYVFNGLVRYDKDLKLEGELAESWEISPDGK
                     RITFHLRKGVRWHDGAPFTSDDVMFTYRRMIDPRTPTAYGEDFKQVKRASAPDPLTFV
                     VEYARPFAPALASWGMHVLPKHLLEKYPDISKSPLNKKPVGTGPYRFVEWKTGEKVVF
                     DASPDYFEGRPFISRVITRVIPDQATMFLELKSGGVDIMTLTPPQYVRQTETAEFSKS
                     FNKYKYTSSGYTYLGFRLSHPFFKDKRVRQAIAHATDKKALIDGVLLGLGQEATGPYK
                     PGTWAYNPSVRKYPHDPVRAKALLAEAGWKEKDGVLVKDGQTFEFTVLTNAGNDARAK
                     TAAILQQNLAEVGIRMKMRTVEWAAFINEFIDKRKFDAVILGWNITPDPDQFDIWHSS
                     KTGPKELNHVGFANPEVDRLLDEGRSTFDLEQRKKAYFRIQEILAEEQPYVFLYVPEA
                     LPVVHNRFRGIAPAPAGITYNFVKWYVPAALQKHRVQP"
     gene            7535..>8230
                     /locus_tag="VF357_10455"
     CDS             7535..>8230
                     /locus_tag="VF357_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7524616.1"
                     /translation="MLRYIARRLLLTVPLLVGISLVSFLMMHMAPGGPIGAGTDLNPK
                     ATAESRARLKAYYGLDQPLHVQYGRWLGRMARLDFGDSFSPDGRPVAEKIKERIPITL
                     TINVLSMGLIFLVAIPVGVYSAVRKGSLFDRISTVAVFTGFAIPTFWLALLMMILFGV
                     KLGWLPISGISSMDYDALGTLGRIADRGRHLVLPVLLAGFGGLAGMSRYMRSNMLEVI
                     QQDYVATARAKGLP"
BASE COUNT         1438 a         2732 c         2750 g         1310 t
ORIGIN      
        1 cgatgatcgt ggtgaccttg gtacccaccc tgcggttttc cttcgtcgcg cggagtccga
       61 tcggcttgcg gaaccatctt accgcaggga acggctcaca aggaaaggag actcaccatg
      121 ccaaggttcg acgccgaata cgtcccccat tccgcgatgg cgatctacgc tcaccccgac
      181 gacatcgagt tcaccatcgc cgggacggtc gcgaaatggg tgcgggcggg ttgcgaggtg
      241 acgttcgtcc tcatcacgag cggaaattcc gggacgcacg accggaagtt cacccggaag
      301 agcctcacgc gggtgcggga acgggaggag cgggaatccg cacggatcct cggcgtcgcc
      361 cgggtcgttt tcctgcgtca cggcgactgc gaactcgtcc cgaccctcgc gttgcggaag
      421 gagttggtcc gccggatccg ccggcaccgc cccgaggtcg tgctgtgcaa cgacccccag
      481 gccctcttct tcggcgatcg gtacatcaac cacccggacc acctcgcggc ggggaaggcg
      541 gcgctcgaag cggtcttccc ctgcgccgag atggagctcc tgtggcccgg ggcgggcctt
      601 ccgcacaagg tccacgccgt ctacgtcagc tcgacctccg ccccggacac ctggatcgac
      661 gtgacggaca cgatcgaaac gaagatcgcg gcgctgacgg cccatcggag ccagctgggg
      721 gaccgggaca tcgcccccga tatcctcgga cgggcgaagg acgaggcgcg gcgggcgccg
      781 aagtcggcgg gaaaaaaagg tccgggccgg ccgaagcacg cggaggcgtt ccgcgtcatg
      841 cggctgctgc gcgacgaacc gacaaaggat aacgactaat ttgggtaatg gcgaccggtc
      901 tgcgcctcgc gggatgcccc ttgcggggga cgccttgcga tgcccatgga tgggcaagtg
      961 ccgcgggagc catggatggc gggagcggcc ccgtccttcg atggacgggt cgcgacaaaa
     1021 agatccgggg gcgtccctgc ccgtcgcgac acttggcctt ccttggccat cggcggcggc
     1081 tcagtcgctc ttcgctcccg gcctccatgc ctccgcttcg tcgctcatgc ccccgccgag
     1141 gcaccccgcc tcggcgcccg accggcctcg tactacgctt cacaattacg gcaacctgtc
     1201 cggaggcgcc gcggagcgtg ataccccttc cgagcgggcg tgagttttcg ccggagccat
     1261 ggagggcgag aggcgaaaac gaccgccagc ggagggcgcc agggatggcg gacggagcgg
     1321 tagcaaggaa gggagtatca cgcggagata gaggcgcctc cggacaacag gtaactgtaa
     1381 ttgtgaaatg atgcgctacg ccctacccgg cgacgcggtt cttgaggtgc ctccggacgt
     1441 accagacgac tcccgccacc aggaccgcgc cgatcagaag gtcgaaccgg tggaaatatt
     1501 cccgcaacgt cggccacttc tcccccagca ccatgccgag atacgccagt ccgaggcacc
     1561 acgggaacga cccggcgatg gtgtagagga ggaaccgctt catgtccatc ctcgccaccc
     1621 cggcgggaaa ggcgatgaag gttcggatca cggggagcag gcgcgcgaag aacacggtcg
     1681 cttcgccgtg gcgggtgaac cacctgtccg ccagatcgag gtcatgacgg gacatgagga
     1741 ggtacttccc gtacttctcg atgaggggac ggccgccgta cattccgagg tagtaggcgg
     1801 gcaccgatcc caccacgcag ccgaaggcgc cggccagcga cacctcccag agagagtgca
     1861 cccccttgta tacgaggtac ccggagaacg gcatgatcac ttcggacggc aggggaatgc
     1921 acgccgactc gatcgccatc aacaggacga tgccgggcag cccgagggag gagatgacga
     1981 agatgacgaa gcccgcgagg gcttcgagga tgcgcgttac catggattca ctcccggaag
     2041 taggatagcg acgttgcggg ttaccccacc gtgatgaacg gcgggccgat ggagagggaa
     2101 ggctgggcgt ctccgacggg gaccccctgg ccgtccttgc cgcacgtgcc gataccgaac
     2161 ccgaggtccg atccgacgcg gtcgatcatc gagaggacct tggggccgtt ccccgtgatc
     2221 gtcgctccgc ggaccggctc cccgcgcttc ccgccttcga tccggtaccc ctccgccacc
     2281 tcgaacatga agtcgccggt gacggtgttc acctggccgc ctcccatctt gacgacctgc
     2341 aacccccggt cggtgccgga aaggatcccc cccggggccg tgttgccggg caggatgagc
     2401 gtgttcgtca tgcgcgggat cggtttgtgc cggtacgact cccgccggcc gttccccgtg
     2461 ggggcggcgc cgtccttcat cgccgacagg cggtcgtgga gaaatccctt caggatcccg
     2521 gcgtcgacca gcaaggtccg ctgcccgggc gtcccttcgt cgtcgatccc gaacgagccg
     2581 cgcttcccgg ggacggtggc gtcgtcggcg atggagacca gcgggctacc gatgcgctcc
     2641 cccagtttct ccttgtagac cgacatcccc cgccgggcga ggtccgcttc cagcccgtgc
     2701 ccgaccgcct cgtggaccat cgtccctccc gcttcggagg agaggacgac cggcattcgc
     2761 ccgccgggga gcttcgccgc gtggagcgcc cgtaccgccc tccctgcggc cccgcgggcg
     2821 agcagcgaag gaacccctcc gtcaaggaac tcgagcccct cggtcccgcc cgccgattcg
     2881 tatcccatcg tcagcccgcc gtcgtccccg gccacggcct ggacggcgag gacgcaatac
     2941 gtctgttcct cccggacgaa gacgccgggg tgggcggcaa cctcgatgtg gcgcagcgat
     3001 tccgaccagg tcgcccggac ctgccgcacc tcacgggaga attcccttgc gatccggtcg
     3061 accttccgaa ccagcgcgac cttctccccg atcccccgcg cggccggcgg cacacggacg
     3121 acggtcggcc cgcggatcga ctgtaccgac gcgggaagaa ccacggcgac gccgtctcct
     3181 tccgcgtagc gggagaggtc gctcgaaagg gcgagaaggg attcggccga ccggtcgttc
     3241 gtgtaggcgt aatacgtttt tccgcggaag aggagtcgaa cgccgatccc cgcgtcggtc
     3301 cccgaaacga cccgttcgat ccggccatcc tccatgagga ccgtgaggga ccgggcttcc
     3361 tcgaaaaaca gttccccgtg ttcaccgccg cgtgacagca gcgcggagag gattctcgaa
     3421 acgggcagtt cggagagcgc agcgttctcg gacatcggtc ctcctcgcgg tttcccgggg
     3481 agtgagtata taatacaaat ggttgatatt ttaccggagg aacggatgcg aaccatcatc
     3541 gccacggggg ccttggccct ccttcttttt ctcgcccccg cgggggcgtc caacggggtt
     3601 gccggcccga ttccccccgg gcccgatgcc cgcgcctacg ccaactttct cgccggatat
     3661 ctcgactccc gggaggggaa cctggacggc gccctcgggt cgtacaagaa ggcgctgaag
     3721 tacgccgggg acgagcccga cattctctac gagatcgcca acgtactcgt gaagaaaggt
     3781 cggctccccg aggcccaagc ggaactggaa aaggttctgg cggcggacga ggggcacacg
     3841 cggtcgcggt atcttctggc ggggatcctc gccgcgtcgg gcgaacgcga aaaggccctc
     3901 gccgattacg gacgcgtctt gaaggaggac ccggagaacg atgacgcgta cctccacatc
     3961 gccaccctct acgcggagcg gggggatttt tccaaagcag aggaagtcct cggtacgctg
     4021 atcgcccggg accccgaatc ctaccttgcg tactattacc ggggccgcgt ccttgcggcc
     4081 cagaagaagt tcgaggaagc actgaccgac tacgacaagg tcctctcgat cgccccggcc
     4141 ttcgacgcag cgctcctcga gtccggggcg gttctcgaga tcctcggccg gaacgcggag
     4201 gcggaggagc ggtacaagaa ggcgctccaa tcttccccga acaacgcgtt catccgggac
     4261 cggctcggca gcctgctgat ccgggagaag aagatcgacc aggcggtggg cgagtacgag
     4321 gaattgaaga aattctccgc cgccaacccc gacgtccgca cgaagctggg acttctctac
     4381 ctcgatcgcg accggttcga cgacgcgatc aacgagttca ccttcgttct cgcctccgat
     4441 ccgggaaatt cgcaggtgcg attcttcctg gggacggcgt atgaggagaa gggcgccgtg
     4501 ccggaggcag aggcggcctt ccggaagatt cccgaggtcg acccggtgca ccgggaggcg
     4561 atgctccacc tcgcaatgct cctgtcgcgg cagaaaaagt ccgatgaggc gatcgaggtc
     4621 gtccggaaac ttcgggagaa gagccccggg gacgtggagc tgatgatctt cctcgccggg
     4681 cagctcgagg gggcgaagcg gtacgacgag gcgttggccg tggccagcga ggcgacggaa
     4741 aaggctcccg aaaaccccgc cgcgtggttc gcggtcgggg tggtccagga caagctgggc
     4801 aagctcgacc aggtgatcgc cgcaatggag aaggtgatcg ccatcgaccc gaagcacgcc
     4861 accgctctca actaccttgg ctacaccttc gcggaccgga acatgcgcct tcccgaggcg
     4921 gagaagctca tcctgcgggc gctggagatc cgccccgacg acgcgtactt cctcgacagc
     4981 ctcgggtggg tccactaccg gcgaggcgat taccggcgtg ccgaggagga actcctccgg
     5041 gcgctgaagc tcgtccccga cgacccggtc ctcctggagc acctcgggga tgtcctgcag
     5101 gcccagggga gggacgctga ggcggccgcc cggttcgaga aggcgatcgc gaaaggacac
     5161 gagaagccgg acgaagtgaa ggcgaagctc catcgcctgc gcaaggcggg gcccgccggg
     5221 aagtgagatt ccggagcacg aagccgctgc gggccttcgc gcctctcctg gcagtcgttg
     5281 cgctttgcgc gtgcgccccg tcgcggatga tcgtcacggg tccggaagcg gaccacgcgg
     5341 aacggttctt cgccggcctc ccgggacggg tcgtctttcc cgtcaaggcg tcgttttccg
     5401 ggaccgcggt gcccgtcgcg ggggacgccg tccccttcgt ggccgggata tccgcgccgt
     5461 caccggaaga ggagacggtg ggtctgtacg atcccctcgg gcacggggtg gcgtttcttg
     5521 cgaatgacgg ccgccgcgtg gcgatttccc gtggtccggc ggcggacctt gccggattcc
     5581 gcttcgctgc tcccctcgac gcgggtcccg tgtcgctggc gcggatcctc tccggcgccc
     5641 ccggctacgc ggttgcgtcc gcggaggcgg cgcgatacga agacggcgcc tggtcgctct
     5701 ccgacagccg tcagacgctg cgctccgacc cggggcggcg gtttctcgcc ggggccgatt
     5761 accgggtccc ggggatgcgc gtgacggtcg actaccccgg gcggaagtcc gcggacccgc
     5821 cggatcggat cgtcttgtcg gtccgggggg tgacgttcac actgaggagg gatgccgagt
     5881 gagcctgctc ccgcgtcggg ccgttttgcc gtcgattgcg ctgtttcttc tgttcgccgc
     5941 gtgcggcgga gggaagccgg agagcgcggg gaagggagcg gccgtctccg acgtcccggc
     6001 gtacggggac gccatcgtcg aggggagcat cggggacgtg agcggcttcc tcaccgcggt
     6061 cacgaccgac gcctcctccc acgaggcggc aggctacgtc ttcaacgggc tggtccggta
     6121 cgacaaggat ttgaagctcg aaggggagct cgccgaatcg tgggagatct cccccgacgg
     6181 taagcggatc accttccacc tcaggaaagg ggtgaggtgg cacgacggcg cccccttcac
     6241 ctccgacgac gtgatgttca cgtaccggcg gatgatcgac ccgcgtaccc ccaccgcgta
     6301 cggggaggat ttcaagcagg tcaagcgcgc gagcgctccc gatccgctca ccttcgttgt
     6361 ggagtatgcc cgacccttcg cccccgcgct ggcgtcgtgg gggatgcatg tcctgccgaa
     6421 gcacctgctg gaaaaatacc ccgacatttc gaagagcccg ctgaacaaga agccggtcgg
     6481 caccgggccg taccggttcg tcgaatggaa gaccggggaa aaggtcgtct tcgatgccag
     6541 cccggactac ttcgagggga ggccgttcat ctcccgggtg atcacgcgcg tcatccccga
     6601 ccaggctacc atgttcctcg agctcaagtc cggcggcgtg gacatcatga cgctcacccc
     6661 gccgcaatac gtccggcaga ccgaaacggc cgagttctcg aaatcgttca acaagtacaa
     6721 gtacacttcg tcggggtaca cgtatctcgg ctttcgcctc tcccacccct tcttcaagga
     6781 caagcgggtc cggcaggcga tcgcccacgc gacggacaag aaggcattga tcgatggggt
     6841 cctccttggc ctgggccagg aggcgacggg accgtacaag cccggaacct gggcatacaa
     6901 cccgagcgtc cggaagtacc cgcatgatcc ggtgcgggcg aaggcgctgc tcgccgaggc
     6961 ggggtggaag gagaaggacg gggtgctcgt gaaggacggg cagacattcg agttcacggt
     7021 gctcaccaac gcggggaacg acgcgcgtgc gaagacggcg gcgatcctgc agcagaacct
     7081 ggccgaggtc gggatccgga tgaagatgcg gacggtcgag tgggcggcct tcatcaacga
     7141 gttcatcgac aagcggaagt tcgacgcggt catcctgggg tggaacatca ctcccgatcc
     7201 cgatcagttc gacatctggc actcctccaa gaccggcccg aaggaactga accacgtcgg
     7261 gttcgcgaac cccgaggtgg accgccttct cgacgaggga cgaagcacgt tcgacctcga
     7321 acagcggaaa aaagcgtact tccggatcca ggagatcctg gccgaggagc agccgtacgt
     7381 cttcctttac gtccccgagg cgctaccggt ggtgcacaac cggttccgcg gcatcgcgcc
     7441 ggccccggcc ggcatcacgt acaacttcgt caagtggtac gtcccggcgg cgctccagaa
     7501 gcaccgggtc caaccgtaag ccgacggaga cgcgatgctc cggtatatcg cgcgccggct
     7561 cctgttgacc gtccccctcc tcgtcgggat cagcctcgtc tcgttcctca tgatgcacat
     7621 ggcccccggg gggccgatcg gcgcggggac cgatctcaac ccgaaggcga cggccgaatc
     7681 gcgggcgcgc ctcaaggcat attacggcct cgaccagccg ctccacgtcc agtacgggcg
     7741 gtggctcggc cggatggcga ggctggattt cggcgacagc ttctcccccg acgggcggcc
     7801 ggtggcggag aagatcaagg aacggatccc gatcacgctg acgatcaacg tcctgtcgat
     7861 ggggctcatc ttcctggtgg ccatccccgt gggcgtctac tcggcggtgc gcaaggggtc
     7921 gctcttcgac cggatctcca cggtggccgt cttcaccggg ttcgcgatcc ccaccttctg
     7981 gctcgccttg ctgatgatga tcctcttcgg ggtgaagctg ggctggctcc cgatctccgg
     8041 gatctcgtcg atggactacg acgcgctcgg gacgctcggg cggatcgccg accgcgggcg
     8101 gcacctggtc cttcccgttc tgctggcggg gttcggaggg ttggcgggga tgtcgcgtta
     8161 catgcgatcg aacatgctcg aggtgatcca gcaggactac gtcgcgaccg cccgggccaa
     8221 ggggctcccc
//
LOCUS       DASVJL010000507         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1030406, whole genome shotgun sequence.
ACCESSION   DASVJL010000507 DASVJL010000000
VERSION     DASVJL010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030406"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VF357_10460"
     CDS             complement(<1..192)
                     /locus_tag="VF357_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524617.1"
                     /translation="MLGFGGAGVALQIVRRRVERSPGRFFAWMILLAAPAFDLAFRAS
                     QLVSFDPFLLLWDPVAWPRF"
     gene            complement(196..372)
                     /locus_tag="VF357_10465"
     CDS             complement(196..372)
                     /locus_tag="VF357_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524618.1"
                     /translation="MSTAAPRDRGDLFSADAQAERVVPAVFFLSAATLAYEIVLFRLL
                     SFRFWSHFVPLIVS"
     gene            complement(385..660)
                     /locus_tag="VF357_10470"
     CDS             complement(385..660)
                     /locus_tag="VF357_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524619.1"
                     /translation="MVRDQIEREGVTDPRVLAALRDVPRHLFVSPSFRAKAYEPYPLP
                     IGEGQTISQPYVVCFMTQNLVFIEKDARGSITHRNVLPVAFVRLHGH"
     gene            complement(1087..1884)
                     /locus_tag="VF357_10475"
     CDS             complement(1087..1884)
                     /locus_tag="VF357_10475"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7524620.1"
                     /translation="MHWFSGIIGEYGGLALFLAGFLGNIGLPIPAFPVLILAGALSLE
                     SNLSIPGAMVGAAAGAASASGLWYHLGRWRGRMVLFTLCRMSLNPDACMERAIFEFHR
                     RPSTMVLFSKFLPGVNSIVPPMAGIVSMPFHRFMILYLIGAFMWAGVGVGIGWAFGVQ
                     IVRQGGNVYGALGWILAGGIAAFFAWRAAYRYYLVHRFSVPRLQPLELHQKMTTGGDP
                     LVLDLRIDLAFDKATGMLPGARRIRPATFQKHVHELPRDRELVFYCT"
BASE COUNT          502 a          731 c          745 g          382 t
ORIGIN      
        1 gaatcgcggc caggccacgg gatcccaaag gagcaggaac gggtcgaacg agacgagctg
       61 cgaggcgcgg aacgcgaggt cgaacgccgg cgcggcgaga aggatcatcc atgcgaagaa
      121 ccggcctgga gacctttcaa cacgccgccg gacgatctgg agcgcgaccc cggcgcctcc
      181 gaatccaagc atcgcctatg agacgatcag cgggacaaag tgcgaccaga accggaacga
      241 caggaggcga aagaggacga tctcgtacgc cagcgtggcc gccgaaagga agaagacggc
      301 cggaacgact ctttccgcct gcgcgtccgc ggaaaacaga tccccgcggt cccttggcgc
      361 tgcggtggac aaacgggagc cccgtcagtg gccatggagg cggacgaagg cgacgggaag
      421 aacatttctg tgcgtaatcg agcctcgggc gtccttctcg atgaagacga ggttctgggt
      481 catgaagcag acgacgtacg gttgcgagat cgtctgccct tccccgatgg gcagagggta
      541 aggttcatac gccttcgcgc ggaacgaagg cgaaacaaag aggtgccgcg ggacgtcccg
      601 caacgcagcc aggacgcgcg gatcggtgac cccttcgcgc tcgatctgat cgcgcaccat
      661 cgccgcccgc ttctgaagga cggctcgtcc gacgccaacg ccgggtggag cgcagcggac
      721 aggatggccg cggcgatcgt gacaagacga aggaagccca tctccctcca cgggtccgac
      781 gtggaacgca tggcgtcaaa agggatggca atttccatgt cttccgcttc tcccaaagcg
      841 ccagaattga ctccctacgt acgttgtaaa caatatcaac tgtatggttt ttgtatagtg
      901 gacctcccga aacacacgcg gccggggccg ggcgccccat cacgcggatg gagcggccgc
      961 ggaggtggat tgcgtgggaa acccctcacg ggtccaggcc tcgaaccctc ccagcaggac
     1021 gctcacgttg tggtgcccct tcttgatcat atgccgcgcc agactggcgc tggtggcctc
     1081 gccgggtcag gtgcagtaga agacaagctc gcggtcgcgg gggagttcgt gcacatgttt
     1141 ttggaacgtc gctggacgga tcctccgggc ccctggtagc attccggtgg ccttgtcaaa
     1201 ggcaaggtcg atccggaggt caagcaccag ggggtcccct cccgtcgtca ttttttggtg
     1261 gagttccaac ggctgcaggc gcgggacgga gaagcggtgg acgaggtaat aacggtaggc
     1321 ggcgcgccat gcgaagaagg ctgcgattcc acccgcgagg atccacccca gtgcaccgta
     1381 gacattgccg ccttgacgga cgatctggac gccgaaggcc cagccgatcc ccacacctac
     1441 gcccgcccac atgaaggcgc cgatcaggta gaggatcatg aagcggtgaa acggcatgga
     1501 aacaatcccc gccatcggcg gcacgatcga gtttaccccg gggagaaatt tcgagaagag
     1561 aaccatggtc gacggccggc ggtggaactc gaagatcgca cgctccatgc aggcgtcggg
     1621 gttgagcgac atcctgcaaa gtgtgaacag gaccatcctg cctctccagc ggcccaggtg
     1681 ataccagagg ccgctcgcgg acgccgcccc tgccgctgcc ccgaccatcg ctcccgggat
     1741 agagagattg ctttccaacg agagggcacc ggcgagtatc agcaccggaa aggcaggtat
     1801 cggaaggccg atgtttccca ggaagcccgc gagaaaaagg gccaatccac cgtactctcc
     1861 aatgattccc gaaaaccagt gcacccgacc cccttcccgt gaatgatctt accacgaagg
     1921 ccgaatgcac ccagccctcg gccggatcta ggtgcggata atctctcgcg gcaccgatgt
     1981 gggggtcgcg ctttcctgtg tgataaagtg aaaatatgaa cggccctttg ctgatcagcc
     2041 ttctaccacc ggagcaaatc tgggactccg cggccgccac ttccggctca acgccattcg
     2101 tcgccattct cctgctgatc ggattcctcc tgtccctgtt tctgcgccat ccgggacgtt
     2161 gacagggagt ctgcaaccgc actcactgcc gcctgacgcg atccaagtcg atgaacggag
     2221 gcaaccgatg ttcgacgttg tcgggatcgg actcaacgcg gtcgattacc tcgtgggcct
     2281 tccgaggttc ccggtgccgg gcgagaaact gcggatgtcc gctttcgcac gagaaggcgg
     2341 cggccaggtg gcaaccgccc
//
LOCUS       DASVJL010000508         3834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1594236, whole genome shotgun sequence.
ACCESSION   DASVJL010000508 DASVJL010000000
VERSION     DASVJL010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594236"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..199
                     /gene="rpsU"
                     /locus_tag="VF357_10480"
     CDS             2..199
                     /gene="rpsU"
                     /locus_tag="VF357_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX7524621.1"
                     /translation="MPGVRVKEEEPFESVLKRFKKQCEKAGILSEIRKREHYEKPSVK
                     KKKKTLAARKRALKKLKKMAR"
     gene            225..677
                     /locus_tag="VF357_10485"
     CDS             225..677
                     /locus_tag="VF357_10485"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX7524622.1"
                     /translation="MHKDMQTAAKERNSLALSALRMAVAAVQNREIEAVTRKEMPQGG
                     TLPEETILKVLAAMVKQRRESIGLFLQGNRPELAEKEESEIVVLERYLPKALTAAEIE
                     AAVREAIAETGAGLPSDMGRVMKALMSKVAGRADGKAVSETVRRLLAG"
     gene            706..2457
                     /gene="dnaG"
                     /locus_tag="VF357_10490"
     CDS             706..2457
                     /gene="dnaG"
                     /locus_tag="VF357_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX7524623.1"
                     /translation="MGGRISESTIREIRDRADIVEVVSETVPLSRAGASFRGLCPFHR
                     EKTPSFYVHPARQAFKCFGCGEGGSVFHFLMKARNLSFTDSVEELAERFGVAVRYEGG
                     AVRSRPKEDLYALLRLAADTYRELLGSPAGKAGREFLRRRGVTPEAEREFALGWCGTG
                     GELVSALKREGIDPARGEAAGLLLPSGNGYRDRFRGRVLFPIGDARGRICGFGGRAVD
                     NAVPKYLNSPESETYRKSSLLYGLYQALPALRSEGRVVVVEGYMDLIGLWQKGVRGVV
                     ATCGTSLTESHARTLKRLSENVILFYDGDVAGKKAAVRSGGPLYAAGVSPKALFPPKG
                     MDPDDWAKSAAPEELTRRIAGAVPLMESIERGASRKYDLATLSGKLSYVQLMAKYLRW
                     VTEPAERELYAQRVAQTSGLPVDTIHRQVGPSVPPPLPEGGAPPRKKDSRGEESHLVR
                     LLAVDPSLAVTARGDGVGELLSGEDAREVVAHLADLAERTPGEAGFPLGEDLSDGARK
                     LLSAELVLADVSPEEARRRYPGAVLSLRIRRAREETEELQEKVKGASGEEASLLFDRV
                     VAAKRELERLESERRSR"
     gene            2473..>3834
                     /locus_tag="VF357_10495"
     CDS             2473..>3834
                     /locus_tag="VF357_10495"
                     /inference="COORDINATES: protein motif:HMM:NF016433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7524624.1"
                     /translation="MARRKQPDGIDGLVEKGRAQGYVTYDELNSALPPDVLTGDQIED
                     VMAIFGANAIEVVDDFQKVPLPPGANLLIGEEEAGEEEEAAVEEEPAVEEEEFGYPGG
                     VKGNDPVRLYLKEMGAIHLLNREGEVSLAKRIEDGEREIVSAVKGCSIALADLFKVGE
                     KLKAGEMQIRDVIKDLDDDSTEEEEKEALDKVLRIVQRIRKTYQSALEIEQRLRKGGL
                     KEKDRQALKTRHASLNERVVESIQEIHLKERQIEVIGDKIRRFADRIEAAESRIYDCC
                     ARLRLTEKECSRLFKEIHAKKDGLAKAASKYHVKKEKLVEIEETLKESRAVVRKVERE
                     AGLSAGQIQETLAAIEQGERKVREAKRELVEANLRLVVSIAKKYTNRGLQFLDLIQEG
                     IIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRT
                     SRYL"
BASE COUNT          743 a         1112 c         1399 g          580 t
ORIGIN      
        1 catgccgggc gtccgcgtga aggaagagga gcccttcgag agcgtactga agcgattcaa
       61 gaagcagtgc gagaaggccg ggatcctttc ggaaatccgc aagcgggaac attatgaaaa
      121 gcccagcgtc aagaagaaga agaagacgct ggcggccagg aaacgcgcgt tgaagaaatt
      181 gaaaaagatg gccaggtagg cgctgattgg gacttaagga acagttgcat aaggatatgc
      241 agacggcggc caaggagcgt aactccttgg ccctttctgc tttgcggatg gctgtggccg
      301 cggtgcagaa ccgggagatc gaggcggtca cccggaagga gatgccgcag gggggaaccc
      361 tccccgagga gacgatcctc aaggtgctcg ccgcgatggt caagcagcgc cgcgaatcga
      421 tcgggttgtt cctccagggg aaccgcccgg agctcgccga gaaagaggag agcgagatcg
      481 tcgtcctcga gcggtacctc ccgaaggcgc tcaccgcggc agagatcgag gcggccgtgc
      541 gcgaggcgat cgcggaaacc ggcgccgggc ttccgtccga catgggacgc gtcatgaagg
      601 cgctcatgtc gaaggtggcg ggccgggccg acggcaaggc ggtcagcgaa acggttcggc
      661 gcctcctggc gggatagcgg tccgggggcc gctccaggga gacctttggg aggtcggatc
      721 tccgaaagca cgatccggga aatacgggac cgggcggaca tcgtagaggt ggtctccgag
      781 acggtccccc tgtcccgggc cggggcgagt ttccgcggtc tgtgcccctt ccaccgggaa
      841 aagaccccgt cgttctatgt ccaccccgca cggcaggcgt tcaagtgctt cggctgcggg
      901 gaggggggat cggttttcca cttcctgatg aaggcgcgca acctttcctt cacggactcg
      961 gtcgaggaac tcgcggagag gttcggcgtg gcggtccggt acgagggtgg agccgtccgt
     1021 tcgcggccga aagaggatct ctacgctctt ctccgcctcg cggcggatac gtaccgggaa
     1081 cttctgggat cccccgccgg gaaggccggg agggaatttc tgcggcggcg gggagtgact
     1141 cccgaagccg agcgggaatt cgctctcggc tggtgcggca cgggcgggga actggtttcc
     1201 gcgctgaagc gggaggggat cgacccggcc cggggcgagg cggcaggcct actgctccca
     1261 tccgggaacg ggtaccggga tcggttccgg gggcgcgtgc tcttcccgat cggggacgca
     1321 cggggtcgca tctgcggttt cgggggtcgg gcggtggaca acgccgtccc gaagtacctc
     1381 aattcccccg agtccgagac gtaccggaaa agttccctcc tgtacggcct gtaccaggcg
     1441 ctcccggccc tccggagcga gggccgcgtg gtggtggtcg aggggtacat ggacctgatc
     1501 ggcctttggc agaaaggggt ccggggcgtc gtggcgacgt gcgggacctc gcttacggag
     1561 agccacgcca gaactctgaa gcgtttgtcg gaaaatgtca tcctctttta cgacggcgac
     1621 gtggccggga aaaaggctgc cgtacggtcc ggaggtcctt tgtacgcggc gggggtgagc
     1681 ccgaaagccc tgtttccccc taagggaatg gatccggacg actgggcgaa gtcggcggcg
     1741 ccggaggagt tgacccggcg tatcgccggg gcggttcccc taatggaatc gatcgagcgc
     1801 ggggcttcca ggaagtacga tctcgcgacc ctttccggaa aactttcgta tgtgcagttg
     1861 atggcgaaat atcttcgatg ggtgaccgaa ccggcggagc gcgagcttta cgcgcagcgg
     1921 gtggcacaga cttcggggct ccccgtggac acgattcacc ggcaggtcgg gccgtcggtc
     1981 cctccgcccc ttccggaagg aggcgccccg ccgcggaaga aggattcccg tggcgaggag
     2041 agccacctcg tgcggttgct ggccgtggac ccctccctcg cggtcacggc gcgcggggac
     2101 ggggtcgggg agctcctctc cggagaggac gcccgggaag tcgtcgcgca tctggcggac
     2161 ctggccgagc ggacccccgg ggaggcggga ttcccgctgg gggaagatct ttccgacggc
     2221 gctcgaaaac ttctgtccgc cgagctcgtg ctggcggacg tttccccgga ggaggcgcgc
     2281 aggcgctacc cgggggcggt cctttccctc cggatccgcc gggcgcggga ggagaccgag
     2341 gagttgcagg agaaggtgaa gggtgcgtcg ggcgaggagg cttcgctgct cttcgaccgg
     2401 gtggtggccg cgaagcgcga gctggagcgg ctggaatcgg aacgcagatc ccgttgacga
     2461 ggtgaagcac agatggcgag gcggaaacag ccggacggca tcgacggact ggtggaaaaa
     2521 gggcgggcgc aggggtacgt cacctacgat gagctgaaca gcgcccttcc gcccgacgtg
     2581 ctcacggggg accagatcga ggatgtcatg gcgatcttcg gcgcaaacgc gatcgaggtg
     2641 gtggacgact tccagaaagt ccccctccct cccggcgcca acctcctgat cggcgaagag
     2701 gaggccggcg aggaggagga ggcggccgtg gaggaggagc ccgccgtcga ggaggaggag
     2761 ttcgggtacc ccggcggggt gaaggggaac gaccccgtgc gcctctacct caaggagatg
     2821 ggcgccatcc accttcttaa ccgcgagggc gaggtcagcc tcgcgaagcg gatcgaggac
     2881 ggagagcggg agatcgtctc cgcggtgaag ggctgttcca tcgcgctggc cgacctgttc
     2941 aaggtcggcg agaagctcaa ggcgggcgag atgcagatcc gggacgtcat caaggatctc
     3001 gacgacgact ccacggaaga agaggagaag gaggcgctcg acaaggtatt gcgcatcgtc
     3061 cagcggatcc ggaaaaccta ccaatccgcc ctcgagatcg agcagcgcct ccggaagggc
     3121 ggcctcaagg aaaaggaccg gcaggcgctg aaaacccggc atgcctccct caatgagcgc
     3181 gtggtggagt cgatccagga gatccacctc aaggagcgcc agatcgaggt catcggcgac
     3241 aagatccgca ggtttgccga ccggatcgaa gccgcggaga gccgaatcta cgactgctgc
     3301 gcgcggttga ggctcacgga gaaggagtgt tcccgcctct tcaaggagat ccatgcgaaa
     3361 aaggacggcc ttgcgaaggc cgcctcgaag taccacgtga agaaggaaaa gctcgtcgag
     3421 atcgaggaga ctctcaagga gtcgcgggcc gtcgtccgga aggtggagcg ggaggcgggg
     3481 ctttccgcag ggcagatcca ggagacgctc gccgccatcg agcaggggga gcggaaagtc
     3541 cgcgaagcca agcgggagct ggtcgaggcg aacctccggc tggtggtctc gatcgcaaag
     3601 aagtacacca accggggcct ccagttcctg gatctcatcc aggaggggat catcggcttg
     3661 atgaaggcgg tggacaagtt cgagtaccgc cgtgggtaca agttctccac ctacgccacc
     3721 tggtggatcc gccaggcgat cacgcgggcg atcgccgacc aggcgcgaac catccgcata
     3781 ccggtccaca tgatcgagac gatcaacaag ctcatccgga cctcccggta cctc
//
LOCUS       DASVJL010000509         6185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1781021, whole genome shotgun sequence.
ACCESSION   DASVJL010000509 DASVJL010000000
VERSION     DASVJL010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781021"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2551)
                     /gene="ppdK"
                     /locus_tag="VF357_10500"
     CDS             complement(<1..2551)
                     /gene="ppdK"
                     /locus_tag="VF357_10500"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX7524625.1"
                     /translation="MAVKRVFFFGGGKAEGNGAMRDVLGGKGAGLAEMTNLGVPVPPG
                     FTIGTEVCNLYYRNHGKLPQDVKAEIAANLAKLERAIGKKLGDPKNPLLVSVRSGAKF
                     SMPGMMDTVLNLGLNDKTVEGLAQKSRNPRFAYDSYRRFLMMFSDVVLDLPKSGFEDI
                     FDAKKQERGAANDVDLTAEDLMNVCGMFKALVKERLKREFPQDARIQLELARDAVFRS
                     WNNDRAKYYRKANGIPDDIGTAVNVQAMVFGNMGDDCATGVGFTRNPATGAKEFYGEY
                     LVNAQGEDVVAGIRTPHPIRDMKKEFPKVFDQLVRITAKLEKHYKDVQDFEFTIESDT
                     LYMLQTRNGKRTAAAAVKIAVDMVKEKLITKEEALLRFEPQQIDQLLHPVIDPKAKLD
                     VVAKGLPASPGAATGEAVFHAVKAVEWATEGKDVILVRKETSPDDIHGMDVARGILTA
                     KGGMTSHAAVVARQMGRTCVVGCDTIDIDETTNRFMVGGKVVREGDFISLNGTTGEVI
                     LGKAPLIAPAMTDAFEVFMSWADAVRRLKVRANADTPRDARVARNFGAEGIGLCRTEH
                     MFFAEDRIPIMQEMILAGSREDREAALSKLLPMQRDDFKGLYREMKGYPVTIRLLDPP
                     LHEFLPKREELMVEVTKLQLIHADRSIIEEKKRLLERVEELHEFNPMLGLRGCRLGIC
                     YPEITRMQARAIFEAACEVTRDGIRVQPEVMIPLVSLVGEMRAQKEIVVAVAEETMRR
                     HKKKFPYTVGTMIELPRAAVTADEIATEAEFFSFGTNDLTQTTFGFSRDDSGKFIQHY
                     MNRSELCQQCGTKLEKALSCAVCKVTYTKRAENILEADVFATLDQGGVGQLV"
     gene            complement(2567..4657)
                     /gene="glyS"
                     /locus_tag="VF357_10505"
     CDS             complement(2567..4657)
                     /gene="glyS"
                     /locus_tag="VF357_10505"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX7524626.1"
                     /translation="MERDYLLEIGCEEIPAGFVGPALWFGSQQFEGMLKKARLSFRKV
                     DIYGTPRRLTYVIRDLEDRQAASKETVMGPPKSVGWDATGKPTKAALGFARSQGIDVS
                     ALTVFSTDRGDYLGFVREEAARPVQEILPRIVSDFLPAIPFKKSMRWADLDVRFARPV
                     HWIVSLYGDEALPFSFGDVTSGRTTFGHRFLAPAAIDLPSTEAYSGMLAKARVLVDLE
                     DRKGRIRAGIREASIRIGKKWVEDEPLVETVANLVEYPVVMVGRFEEKYLSLPREVLV
                     TSMRNNQKYFVFEDAKGGLFPGFAFVSNMIVPDESVVVAGNERVLRARLSDAEFYYGD
                     DLKKPLFDRSEALKKVLFQADLGTYWEKIERMADIGEFVASFGFPGKAKDCRRAAFLS
                     KADLTTGVIKEFPELQGVMGRHYASMTGESAEIAQSVFEHYLPKGMSDDLPSTDVGAA
                     TAIADKIDLVCGCFGVGLIPTGTADPYGLRRHTLGILSILESRGLRIPIAGLVDRSLA
                     TLAPKLTSPAAEVRKKVLEFFVARYLNLLVSQGAPADLVDAVLAPGLTNVVDLRAKLD
                     ALVAFRADAAFEPLAEVFKRAINITKVYNGPLAVSSMLFEHDEERALHAAAADVSGRV
                     VAAARDGRYGEAFREMAGLQPLVAAFFEKVLVMAKDETVRNNRLALLKGLSAVFASVA
                     DFSKVASSVQPKQG"
     gene            complement(4689..5567)
                     /locus_tag="VF357_10510"
     CDS             complement(4689..5567)
                     /locus_tag="VF357_10510"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX7524627.1"
                     /translation="MFFQDLILSLQKFWADKSCVIHQPYDLEVGAGTFHPATFLQALG
                     PEPWNTSYVQPSRRPTDGRYGENPNRLQHYYQFQVIMKPCPKDYVELYLDSLRAVGID
                     PLKHDIRFVEDDWESPTLGAWGLGWEVWLDGMEITQFTYFQQCGGIDLKPVSGEITYG
                     IERIAMYLQNVNNVFDLKWVGDVTYGDVHHRGEVEFSKYNFELADIPMLFSLFTMYEK
                     ECQSLIAAKLVLPAYDYCLKCSHAFNLLDARGAISVTERTSYIGRVRNLARVCAEGYL
                     KSREEMGFPLLGKFSG"
     gene            complement(5667..>6185)
                     /gene="recO"
                     /locus_tag="VF357_10515"
     CDS             complement(5667..>6185)
                     /gene="recO"
                     /locus_tag="VF357_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX7524628.1"
                     /translation="VLSSASVNRSFWGIVEDWERVRHADHLLEIASELFPQGGAKPRA
                     FDVLLRGMCSIADGEPPPETARRAEAEFLAIGGWGPDLSGCRKCKEKEGRSYRFLDSE
                     GGVLCEACSLRGGAPLSLGAVKTWRALQSGKTAARDRLRIPVMIAEELRAVIPGYVEY
                     CLGKQLRSLGKK"
BASE COUNT         1071 a         2090 c         1863 g         1161 t
ORIGIN      
        1 gcacgagctg cccgacccca ccttggtcca aggtcgcgaa cacgtcggct tcgaggatat
       61 tctccgcccg tttcgtgtag gtcaccttgc acacggcaca ggagagggcc ttctccagct
      121 tcgtgccgca ctgctggcaa agctccgacc ggttcatgta gtgctggatg aactttccgg
      181 agtcgtcccg ggagaacccg aatgtcgtct gggtgagatc gttcgtgccg aaagagaaga
      241 attccgcctc ggtcgcgatc tcgtccgctg tcaccgcggc gcgcggcagc tcgatcatcg
      301 tccccacggt gtaggggaac ttcttcttgt gccgtcgcat cgtctcttcc gccacggcga
      361 cgacgatctc cttctgcgcc ctcatctccc ccaccaggct gaccagcggg atcatgacct
      421 cgggctggac ccggatcccg tcccgggtaa cttcgcacgc cgcctcgaag atcgcgcgcg
      481 cctgcatccg ggtgatttcc gggtagcaga tcccgagccg gcagccgcga agaccgagca
      541 tcgggttgaa ctcgtgaagt tcctcgaccc tctccaggag gcgcttcttc tcctcgatga
      601 tggagcggtc cgcgtggatc agctggagct tggtcacctc gaccatcaac tcctccctct
      661 tggggaggaa ctcgtggagg ggtggatcca gcagccggat agtcacggga taccccttca
      721 tttcccggta cagccccttg aaatcgtccc gctgcatcgg cagcagtttc gacagggccg
      781 cctcacggtc ctcccgggag ccggcgagga tcatctcctg catgatcggg atccgatcct
      841 cggcgaagaa catgtgctcc gtgcggcaca gtccgatccc ctcggcgccg aagttgcgcg
      901 cgacccgcgc gtcccgcggc gtgtcggcgt tggcgcgcac cttgagccgc cgcacggcgt
      961 ccgcccagga catgaagacc tcgaacgcgt cggtcatcgc gggcgcgatc agcggcgcct
     1021 tccccaggat cacctcgccg gtggtgccgt tcagcgagat gaagtccccc tcccggacga
     1081 ccttgccgcc caccatgaac cggttcgtgg tttcgtcgat gtcgatcgtg tcgcacccca
     1141 ctacgcacgt tctccccatc tggcgggcga cgaccgccgc gtgggatgtc atcccgccct
     1201 tcgcggtgag gatcccccgg gccacgtcca tcccgtggat gtcgtcgggg ctcgtctcct
     1261 tgcggaccag gattacgtcc ttcccctcgg tggcccactc caccgccttg acggcgtgga
     1321 agaccgcctc gccggtcgcc gcgccggggg aggcgggaag tcccttggcg acgacgtcga
     1381 gcttcgcctt cgggtcgatc accgggtgaa gcaactggtc gatctgctgg ggctcgaagc
     1441 gcaggagcgc ctcctccttc gtgatcagct tctccttcac catgtccacc gcgatcttga
     1501 cggccgccgc ggcggtccgc ttcccgttcc gtgtctgaag catgtacaag gtatcgctct
     1561 cgatggtgaa ctcgaaatcc tgcacgtcct tgtaatgctt ttccaacttc gcggtgatcc
     1621 gcaccagttg gtcgaagacc ttcgggaatt cttttttcat gtcgcggatc gggtgcgggg
     1681 tgcggatgcc ggcgacgacg tcctcgccct gcgcgttgac gagatactcg ccgtaaaact
     1741 ccttggcgcc cgtggcggga tttcgcgtga agccgacgcc ggtggcgcaa tcgtcgccca
     1801 tgtttccgaa caccatcgcc tggacgttca ccgccgtgcc gatatcgtcc ggaattccgt
     1861 tcgccttccg gtaatatttc gcccggtcgt tgttccacga ccggaacacc gcgtcccgcg
     1921 ccagttccag ctggatccgc gcgtcctgcg ggaactccct cttcaagcgt tccttgacca
     1981 gcgccttgaa catcccgcac acgttcatca ggtcttcggc ggtcaggtcg acgtcgtttg
     2041 cggcaccgcg ctcctgcttc ttcgcgtcga agatgtcttc gaagccgctc ttggggagat
     2101 cgagcaccac gtcggagaac atcatcagga agcggcggta ggaatcgtac gcaaaccggg
     2161 ggttgcggga cttctgcgca agcccttcca ccgtcttgtc gttcagtccg aggttgagga
     2221 ccgtgtccat catgcccggc atggagaact tcgcccccga ccggacggag acgagcagcg
     2281 gatttttcgg atcgccgagc ttcttgccga tcgcacgctc gagcttcgcg aggttcgccg
     2341 cgatctccgc cttgacatcc tgagggagct ttccgtggtt cctgtaatac aggttgcaga
     2401 cctcggtgcc gatggtaaac cccggaggca ccggcacgcc gaggttcgtc atctcggcga
     2461 gtccggctcc cttcccgccg aggacgtccc tcatcgcgcc gttcccttcg gccttacctc
     2521 ccccgaagaa gaagacccgt ttcaccgcca ttcgccgctc ctccgatcac ccttgtttcg
     2581 gctgcacaga agacgccacc ttcgaaaagt ccgccaccga cgcgaacacc gccgacagcc
     2641 ccttcagtag cgcaagccgg ttgttccgta ccgtttcgtc cttcgccatc acgagcactt
     2701 tttcgaaaaa ggcggccacc agcggctgga gccccgccat ttcgcggaac gcctcgccgt
     2761 accgcccgtc tcgggccgcc gccacgacgc gccccgagac atcggccgcc gccgcgtgga
     2821 gcgcccgctc ctcgtcgtgt tcgaagagca tcgaagagac cgcgagggga ccgttgtaca
     2881 ccttggtgat gttgatcgcg cgcttgaaga cctccgcgag gggctcgaac gccgcgtccg
     2941 cccggaaggc gaccagtgcg tcgagcttcg cgcgaaggtc caccacgttc gtgagccccg
     3001 gggccagaac cgcatcgacc aggtcggccg gcgccccttg ggaaaccagg aggttcaggt
     3061 agcgggcgac gaagaattcg aggaccttct tccgcacctc ggcggccggc gacgtgagct
     3121 tcggcgcaag ggtcgcgaga gaccggtcca ccagccccgc gatcgggatc cgcagccccc
     3181 gcgattcgag gatggagagg atccccagcg tgtgacgccg caggccgtag gggtccgcgg
     3241 ttcccgtcgg gatgagtccc acgccgaagc atccgcacac caggtcgatc ttgtcggcga
     3301 tcgccgtcgc ggcccccacg tccgtggacg gcaggtcgtc ggacattcct ttcggcagat
     3361 agtgctcgaa cacggactgc gcgatctcgg ccgactcgcc ggtcatcgaa gcgtagtgcc
     3421 gccccatcac cccctgaagc tccgggaact ccttgatgac cccggtggtc aggtctgcct
     3481 tgctgagaaa cgccgcgcgg cggcaatcct tcgccttgcc cgggaaaccg aaggaggcga
     3541 cgaactcccc gatgtcggcc atccgctcga tcttttccca gtacgttccc aggtccgcct
     3601 ggaaaaggac ctttttcagc gcctcggacc ggtcgaacag gggctttttc aggtcatccc
     3661 cgtagtaaaa ctcggcgtcg gagagccggg cccggaggac ccgctcgttc ccggcgacga
     3721 ccacggactc gtcgggaacg atcatgttcg aaacgaaggc gaaccccggg aacaggcctc
     3781 ccttcgcatc ctcgaagacg aaatacttct ggttgtttct catcgaggtg acgaggacct
     3841 ccctgggcag cgagaggtac ttctcctcga accgccccac catgacgacg gggtactcca
     3901 cgaggttcgc cacggtctcg accagcggct cgtcctcgac ccatttcttg ccgatccgaa
     3961 tcgaagcctc ccggatcccc gcccggatcc tccccttccg gtcttcgagg tccacgagca
     4021 cccgtgcctt cgcaagcatc ccggagtacg cttcggtcga cggaagatcg atggccgcgg
     4081 gggcgaggaa tcggtgcccg aacgtcgtcc gccccgaagt gacgtcgccg aacgaaaacg
     4141 gcagggcttc gtccccgtac agcgagacga tccagtggac cgggcgggcg aaccgcacgt
     4201 cgaggtcggc ccatcgcatc gacttcttga acggaatcgc ggggaggaag tcggagacga
     4261 ttctcggaag gatctcttgt accgggcggg ccgcctcctc gcgaacgaag ccgaggtagt
     4321 cgccgcgatc ggtcgaaaag acggtgaggg cggaaacgtc gatcccctgg gaccgggcaa
     4381 atcccagggc cgccttcgtc ggcttgccgg tggcgtccca tcccacgctt ttcggcgggc
     4441 ccatcaccgt ctctttcgac gccgcctgcc ggtcttcgag gtcccggatc acgtacgtca
     4501 gccgccgcgg ggtgccgtag atatcgacct tccggaagga gagacgggcc tttttcagca
     4561 tcccctcgaa ctgctggctt ccgaaccaga gcgccgggcc gacgaatccc gccgggattt
     4621 cctcgcagcc gatctcgagc agatagtcac gttccatcgg aatccctcat cacaggtaaa
     4681 ctcttgactc agccggagaa cttcccgagc agcgggaaac ccatctcctc gcgcgatttc
     4741 agataccctt cggcgcacac ccgggccagg ttccggacgc gcccgatgta ggaggtgcgc
     4801 tccgtgacgg agatcgcgcc ccgggcgtcg agcaggttga aggcgtggga gcacttgagg
     4861 cagtaatcgt aggcgggaag gacgagcttc gccgcgatga gactctggca ctccttctcg
     4921 tacatcgtga agagggagaa gagcatcgga atgtcggcca actcgaagtt gtacttcgag
     4981 aattccactt ctccgcggtg gtgaacgtcg ccgtaggtga cgtcgccgac ccacttcagg
     5041 tcgaagacgt tgttcacgtt ctgcagatac atcgcgatcc gttcgatccc ataggtgatt
     5101 tccccggata ccggcttcag gtcgatcccg ccgcactgct ggaagtaggt gaactgggtg
     5161 atctccatcc cgtccagcca cacttcccag ccgagacccc acgctccgag agtgggggat
     5221 tcccagtcgt cctcgacgaa ccggatgtcg tgcttcaacg ggtcgatccc caccgcgcgg
     5281 agggagtcga ggtacagctc cacatagtcc tttgggcacg gcttcatgat gacctggaac
     5341 tggtagtagt gttgcaggcg gttcggattc tccccgtagc ggccgtccgt cgggcggcgg
     5401 gacggctgga catatgacgt gttccacggc tcgggtccaa gggcttgcag gaacgtggcg
     5461 ggatggaacg ttccggcgcc gacttcgagg tcgtacggtt ggtgaatcac gcagctcttg
     5521 tcggcccaga acttctgcag ggacagaatg agatcttgga aaaacaccgt tccctcccgg
     5581 aggaagtgat agggatagtt atcatcccac gggaggggtt gtcaagccaa ttcattggaa
     5641 aattgagggg ttatatcact cgaaggtcac ttcttgccca ggctccggag ttgtttacca
     5701 aggcaatact cgacatatcc agggataacg gctcgtaatt cctcggcaat catgacggga
     5761 atccggagtc gatcccgcgc cgcggtcttt cccgactgga gggcgcgcca tgtcttcaca
     5821 gcgccgaggg acagaggggc ccccccccgg agcgaacagg cctcgcacaa gacccccccc
     5881 tccgaatcga gaaaccggta cgaacggccc tccttctcct tgcatttccg gcacccggac
     5941 aggtcgggcc cccacccgcc gatcgcgagg aattctgcct cggcccttcg ggcggtctcc
     6001 ggcggcggct ccccgtccgc gatggaacac atcccccgca ggaggacgtc gaacgcccgc
     6061 ggcttggccc ccccctgggg gaaaagctcc gacgcgatct cgagcaggtg gtccgcgtgt
     6121 cgcacgcgct cccaatcctc cacgatcccc cagaaactcc ggttcacgga ggcggaggaa
     6181 aggac
//
LOCUS       DASVJL010000510         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1311547, whole genome shotgun sequence.
ACCESSION   DASVJL010000510 DASVJL010000000
VERSION     DASVJL010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311547"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..938)
                     /locus_tag="VF357_10520"
     CDS             complement(63..938)
                     /locus_tag="VF357_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524629.1"
                     /translation="MMRGMSLAALAILVLSLSISNASADTPAAYVWPGAELSGVALPS
                     GADGSGPAASGDFGVEDGGAILYLSKDGELRQPPSGKPLPSRSPLKLDAFASGSGVLI
                     GVRGNVLGVIGPGGFSERIVLPGPGYRVATGAGGRIYAFGPARGGSSVLLIENGRALE
                     LIHLTGEAVSDLFVIGERIFFCAGNRIYVAARGEKPALLFMAAGEKVLRSVAPDTRSG
                     VLFFSGDDAVYALRGSSSVSVVKGASGRLRSTARGLFILDAGASRLLLVSGLDRVFAP
                     QEGIAPPDTPTESMK"
     gene            complement(935..2080)
                     /locus_tag="VF357_10525"
     CDS             complement(935..2080)
                     /locus_tag="VF357_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524630.1"
                     /translation="MGVQGIFLQGILGTLMSPSAPKGGNKAQPPPGPSPADLKFKQLL
                     QAEDAVKDAAFPANQQKLLQDLKGAIPNGGPNNIDLKLPPGPLAGRESGGPDAGIDLE
                     VLRAGAGRAFDGNADDSLWMRVHDAGFSPGAPGNPPMSSVSDNPLEYSSSDMKPLDCP
                     FGGAGGTCSVQVSTQPVVSLSSVSVRPAVTSLPPPAAGAGGGTPRLVVQGAEVGDKGL
                     VASVRRTIVDWKSKAQGPELPAEILESVAVSIGEMAMPNMSQQIASEGRDAYKNVVEA
                     YLAKVVEVMTDAAAGRWNDALDKSDAARLKNEGVKTFIEGRTWGKLLWPALEGKPMAV
                     LAAGRDIGMDHAWGEAKEFYGDIPKEKAADAGQKVWDWLLRPKGLPR"
BASE COUNT          394 a          839 c          767 g          383 t
ORIGIN      
        1 tcgccttttc gtatcgatga gacctgttca tgcgcgaacc tccgacaaaa tggttgtcat
       61 cctcatttca tggactccgt gggagtatcg ggcggagcga tgccctcttg aggcgcgaag
      121 acgcggtcga gaccggacac aagaaggagc cgcgacgctc ccgcatccag gatgaaaagt
      181 ccccgcgccg tcgacctcaa cctcccggac gcgcccttga cgaccgagac ggaggaactg
      241 cccctgagtg cgtagaccgc gtcgtccccg gagaagaaga ggaccccgga tcgcgtatcg
      301 ggcgccacgg agcgcaggac cttctctccc gccgccatga aaaggagcgc cggcttctcg
      361 ccgcgcgccg cgacatagat ccggttcccc gcgcaaaaga agatccgctc gccgatgacg
      421 aacaggtcgg agaccgcctc gccggtgagg tggatcagtt cgagcgcacg cccgttctcg
      481 atcaggagca ccgaacttcc gccgcgcgcc ggcccgaagg cgtagatgcg ccccccggcc
      541 cccgtcgcga cccggtatcc cggccccggc aggacgatcc tctccgagaa gcctcccggc
      601 ccgatgacac cgaggacgtt cccgcggaca ccgatcagga cgcccgaccc ggaggcgaag
      661 gcgtcgagtt tgagagggga cctggagggg agcggtttcc ccgacggcgg ttgtcgcaat
      721 tcgccgtcct tcgacaggta aaggatcgcg ccgccgtcct cgacgccgaa gtcgccggac
      781 gccgcgggtc ccgatccgtc cgccccggag gggagcgcga cgccggagag ctccgccccc
      841 ggccatacat acgccgcagg ggtgtcggca gacgcgttcg agatcgacag ggagagaacg
      901 aggatggcga gcgccgccaa cgacattccc cgcatcatct cggaagcccc tttgggcgga
      961 ggagccagtc ccacaccttc tgcccggcgt ctgcggcctt ctccttcggg atgtcgccgt
     1021 agaactcctt ggcctccccc cacgcatggt ccatgccgat gtcgcgcccg gcggcaagga
     1081 ctgccatcgg ctttccctcg agcgccggcc acaggagctt tccccacgtc ctcccctcga
     1141 tgaacgtctt cacgccctcg ttcttcaggc gcgccgcgtc gctcttgtcg agcgcgtcgt
     1201 tccaccttcc ggcggctgcg tctgtcatca cctcgaccac cttcgccaga tacgcctcga
     1261 cgacgttctt gtacgcatcg cgcccctcgg aggcgatctg ctgcgacatg ttcggcatcg
     1321 ccatctcacc gatcgagacg gccaccgatt ccaggatctc ggctgggagc tccggccctt
     1381 gtgccttcga cttccagtcg acgatggttc ggcgaaccga tgcgaccaga cctttatcgc
     1441 cgacctcggc gccctggacg acgagacgcg gggtcccccc tcccgcgccg gcggcaggag
     1501 ggggcaggga cgtgaccgcg ggccggaccg agacggagga aagggagacg accggctgcg
     1561 tcgagacctg gaccgagcac gtcccgccgg cccctccgaa ggggcaatcg agcggcttca
     1621 tgtcggatga ggagtattcg aggggattgt cggacacgga cgacatcgga gggttccccg
     1681 gcgcccccgg agaaaagccg gcgtcgtgaa cgcgcatcca gagtgaatcg tcggcattgc
     1741 cgtcgaacgc ccgtccggcc cctgctcgaa ggacttcgag gtcgattccc gcgtcggggc
     1801 ctccgctctc ccgacccgcc aagggtcccg gcggcagctt gaggtcgatg ttgttcggtc
     1861 cgccgttggg gatggcgccc ttgaggtcct gaaggagttt ctgctggttg gcgggaaaag
     1921 cggcgtcctt gacggcgtcc tcggcctgga gcaattgctt gaacttcaga tccgcggggg
     1981 aggggccggg gggcggctgg gctttgttgc cgcctttcgg ggcggacggg ctcatgagcg
     2041 tcccgaggat cccctgcagg aatatccctt ggactcccaa gttggggctt cccgtcacct
     2101 tgcttccgcc ggacgccgcc ggcggggttt cgccggggca ccatccgtaa cctctgacgc
     2161 attcggggcc ggacgcattc gggatcgaac ccgcgagcac gcccgacggg aagagcgggg
     2221 cgcccgccag aacggccgag agccaaacga tcggaagccc gcgcatcgac cggctacccg
     2281 tccaccccga tgtcattcgc gacctcatga tcggtatcgt tcctctcctc ccggacggcc
     2341 ccttgcgcca gccaccttac cagtatatcg gcgcacggag ccc
//
LOCUS       DASVJL010000511         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_1030984, whole genome shotgun sequence.
ACCESSION   DASVJL010000511 DASVJL010000000
VERSION     DASVJL010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030984"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /gene="tyrS"
                     /locus_tag="VF357_10530"
     CDS             complement(<1..590)
                     /gene="tyrS"
                     /locus_tag="VF357_10530"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX7524631.1"
                     /translation="MENVLQSLKRGTAEVITWEELSRKVAASAREKRPLRVKAGFDPT
                     APDLHLGHTVLIQKLKHFQEAGHQVIFLIGDFTGMIGDPSGKSETRKALTREDVERNA
                     VTYKEQIFKILDPDRTEVRFNSEWLSTLRIEEMVRVAAQMTVARMLERDDFRKRYEEQ
                     RPISIHEFLYPLFQGYDSVALRADVEFGGTDQKFNLL"
     gene            complement(566..1390)
                     /locus_tag="VF357_10535"
     CDS             complement(566..1390)
                     /locus_tag="VF357_10535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX7524632.1"
                     /translation="MWVLFFGDVVGKPGRKAVAEFLSRLRGVRDVDLVIANGENAASG
                     MGLTDSVVRELFESGVDVLTGGNHVWDKKEGIPLVRAGERILRPANYPPGVDGRGWGV
                     FRGRSGTPYAVVSLIGRVFMGNYDCPFRWADAALPGIRREAACVVVDFHAEATSEKRA
                     LALYLDGRVSAIAGTHTHVQTSDASVLPGGTGCITDAGMCGPAGSIIGMDPKTVLRRF
                     LLQVPVRFDVASGEPEASGVFFDLDPETGRSKAVQAFRISESQMRSKETWKTFSNP"
     gene            complement(1574..>1952)
                     /locus_tag="VF357_10540"
     CDS             complement(1574..>1952)
                     /locus_tag="VF357_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX7524633.1"
                     /translation="SAKIVHAIGAHHEDESPKDILPILVQAADALSGARPGARSEMLA
                     NYLKRLEDLEAIAKSFAGVEKSYAIQAGREIRIIVDYQKIGDDAATLLARDIAKKIET
                     DLSYPGQIRVTVIRETRAIEYAR"
BASE COUNT          305 a          703 c          596 g          348 t
ORIGIN      
        1 agcaggttga acttctggtc ggtcccgccg aactccacgt ccgcccgcaa cgccacggag
       61 tcgtacccct ggaagagcgg gtagaggaac tcgtggatcg agatcgggcg ctgttcctca
      121 taccgcttcc ggaaatcgtc ccgctcgagc atccgcgcca ccgtcatctg cgcggcgacg
      181 cggaccatct cctcgatgcg cagggtggag agccactcgg agttgaaccg gacctcggtc
      241 cggtccgggt cgaggatctt gaagatctgc tccttgtacg tcaccgcgtt ccgttcgacg
      301 tcctcccgcg tgagcgcctt ccgtgtctcc gacttgcccg acgggtcgcc gatcatcccg
      361 gtgaagtctc cgatcaggaa gatcacctgg tggccggcct cctgaaagtg cttgagcttc
      421 tggatgagca ccgtgtgccc caggtggagg tcgggcgccg tggggtcgaa tcccgccttc
      481 acccgcaggg ggcgcttctc cctcgcggag gcggcgactt tccgggaaag ctcctcccag
      541 gtgatcactt ccgccgtgcc tcgcttcagg gattggagaa cgttttccat gtctctttgc
      601 tcctcatttg cgattcggag atccggaacg cctgcaccgc tttgctgcgg cccgtttccg
      661 gatccagatc gaagaacacg cccgacgcct ccggctcgcc cgaagcgaca tcgaagcgga
      721 cgggcacctg aaggaggaag cgccgcagga ccgtcttcgg gtccatcccg atgatcgacc
      781 cggcggggcc gcacatcccc gcgtcggtga tgcagccggt gccgcccggc aggaccgaag
      841 cgtcggaggt ctgaacgtgt gtgtgcgttc ccgcgatcgc ggaaaccctc ccgtccaggt
      901 aaagggcgag cgcccgcttc tcggaggtcg cctcggcgtg gaaatcgacc accacgcagg
      961 ccgcctcgcg gcggatcccg ggaagcgccg cgtcggccca gcggaacggg cagtcgtagt
     1021 ttcccatgaa gacacgaccg atcagcgaca ccacggcgta cggcgtgccg ctgcggccgc
     1081 gaaaaacgcc ccatccccgc ccgtccaccc ccggcgggta gttcgccgga cgcaggatcc
     1141 gctctccggc acgcaccagc gggatcccct ccttcttgtc ccatacgtgg ttgccgcccg
     1201 tcaacacgtc gacccccgac tcgaacagct cgcggacgac ggagtcggtc agccccatcc
     1261 cgctggccgc gttctccccg ttcgcgatca cgaggtcgac gtcccggaca cctcgcaggc
     1321 gggagaggaa ctccgccacg gccttgcgac cgggcttgcc gactacatcg ccgaagaaca
     1381 ggacccacat gtaaaacccc tcctaggcac tccgcttcat aaatacgata acgtatccaa
     1441 actgcgatcc cttcggctgc gtcgcctcgg agggcggggc tccgttcgtg gctcgccgtg
     1501 cgatgaaccc gcacggctgc gctttacctc actgcgcccc ccctcctgcg gcgactccgc
     1561 cggccaccct ctgttagcgc gcgtattcga tcgcgcgcgt ctccctgatc accgtgacgc
     1621 ggatctgccc cgggtaggag aggtccgtct cgatcttctt cgcgatgtcg cgggcgagaa
     1681 gagtcgccgc gtcgtccccg atcttctggt agtcgacgat gatgcggatc tcccgccccg
     1741 cctggatcgc gtacgacttc tccacgcctg cgaacgactt cgcgatcgct tcgaggtcct
     1801 ccagacgctt gaggtagttc gccagcatct cgctgcgcgc tcccgggcgg gcgccggaca
     1861 gcgcgtccgc cgcctggacc aggatcggca ggatgtcctt cggcgactcg tcctcgtggt
     1921 gcgccccgat cgcgtgcacg atcttcgcgg ac
//
LOCUS       DASVJL010000512         3580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_653999, whole genome shotgun sequence.
ACCESSION   DASVJL010000512 DASVJL010000000
VERSION     DASVJL010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653999"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /gene="larA"
                     /locus_tag="VF357_10545"
     CDS             complement(<1..1269)
                     /gene="larA"
                     /locus_tag="VF357_10545"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEX7524634.1"
                     /translation="MRPDIAEIVRGADIGGWTPVKIEHGDRTIEVLLPPDAKMLGMRE
                     FAPLAHPADEIRRSLEDPVGTPPLARIIEAKGKPARDLSVCITTSDITRPVPYKGDGG
                     ILDPLLLILHDCGVRRENVMLLIGTGTHRPSTAQEKVAMFGERVAAEFRIIDHDCDDE
                     SMLTDIGRTRSGTVIHINRHFIEADVKIATGLVESHFMAGVSAGRKAICPALVSRKTI
                     ERFHGAQFLSSPKATNLVLQGNPCHEESLEIARKTGVDFIVSTVLNRRLELIGVFSGD
                     LEKSHDKAVELVRRVVSIPVDREYDIVLTHGGYVGINHYQNAKAACNALPILRENGFL
                     ILAACERDQDPIGPATYKTLLHFLKLQGPDGYLAALLNPAWKFTRDQWEPQMWGKVIG
                     KIGEEGMLYCTAFIPKREQRLVPGTAGWDFL"
     gene            complement(1280..1987)
                     /locus_tag="VF357_10550"
     CDS             complement(1280..1987)
                     /locus_tag="VF357_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7524635.1"
                     /translation="MLEIRNASIGYKGGVLAVQDISFSVKKGEVVGLVGSNGAGKSTV
                     LRAISGLLRPRSGEILYNGEALHTVPAYDIVRRGIAMVPEGRQLFGRLSVIDNLLMGA
                     YAIDSKQRTQETLETVLALFPRLAERKGQRAETLSGGEQQQLAIGRGLMSQPSLLMLD
                     EPSLGIMPKLVSEIFETIREISKKGLTVLLVEQNVFEALHISDRAYVLQTGRIVLEGR
                     GEELLKSDLVRQAYLGM"
     gene            complement(1980..2711)
                     /locus_tag="VF357_10555"
     CDS             complement(1980..2711)
                     /locus_tag="VF357_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7524636.1"
                     /translation="MTVAPVLEVEGITKRFGGLTATEDVSFTVGTGEILSIIGPNGAG
                     KSTLFNCITGFYRSDAGKVLFRGKDISTASAHKICKLGIARTFQIVQVISDMTVLENV
                     MTGTFLRTYDRKKVREKAGEILRFAGLYEKKDFLGTDITIADKKRLEIAMALGTEPTL
                     LMLDEAMAGLTPVELREMIGLLRKVREKGVTLIIVEHVMEAVMQLSDRVVVINSGRKI
                     VEGAPAVVVRNPEVIQAYLGDRYHA"
     gene            complement(2708..>3580)
                     /locus_tag="VF357_10560"
     CDS             complement(2708..>3580)
                     /locus_tag="VF357_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7524637.1"
                     /translation="THVMIMVFLFAMMGVAWNILGGYAAMFSFGQAAFFGIGAYTSTI
                     LLMNYNVNPWIGLVAGGVLAALMGAAIGIPCSNLRGHYFAIASIAFAEIVRIHFNNWK
                     FVAAAEGLSLPLLPESLGNFMFHSSKVPYYYIILVFLLLSVLICWYISTNRMGFYLKA
                     IKESHDLAKGMGISFVRYRLYAIMISAFLTAMAGSFYAQYILYIDPESVFILPISVQV
                     VLVAMLGGAGTIIGPVIGAAILLPLSEITRVWLGYKGTGIDMIIYGALIMGISAYQPK
                     GVWGFLTSIRRRAK"
BASE COUNT          625 a         1144 c         1139 g          672 t
ORIGIN      
        1 aagaaagtcc caccccgccg tcccgggcac cagccgctgt tcccgcttcg ggatgaaggc
       61 ggtgcagtaa agcatcccct cctcgccgat ctttccgatc acctttcccc acatctgagg
      121 ctcccactgg tccctggtga acttccatgc cgggttgagc agcgcggcga ggtagccgtc
      181 gggcccctgc agtttcagga aatggaggag ggtcttgtag gtggccgggc cgatcgggtc
      241 ctggtcccgc tcgcacgccg ccaggatcag gaagccgttc tcccgtaaga tggggagggc
      301 gttgcatgcc gccttcgcgt tctgatagtg attgatcccc acatacccgc cgtgggtgag
      361 gacgatgtcg tattcccggt ctacggggat cgacacgacg cggcggacca gttccaccgc
      421 cttgtcgtgg gacttttcga ggtcgccgga gaagacgccg atcagctcga gacgtcggtt
      481 caggacggta ctgacgatga aatccacccc ggtcttgcgg gcgatctcca gcgattcctc
      541 gtgacaaggg ttcccttgga ggaccaggtt cgtggctttc ggggaggaga ggaactgcgc
      601 gccatgaaaa cgctcgatcg ttttccggct gacgagggcg ggacaaatgg cctttcgccc
      661 ggccgacacc cctgccatga agtggctctc caccagcccg gtggcgattt tcacatccgc
      721 ttcgatgaag tggcggttga tgtggattac cgtgccgctg cgggttctgc cgatgtccgt
      781 cagcatagac tcgtcgtcgc aatcgtggtc gatgatccgg aactccgccg ccacccgctc
      841 gccgaacatc gccactttct cctgcgcggt gctggggcgg tgggttcccg tcccgatgag
      901 gagcatcaca ttttcccttc ggacgccgca atcgtggaga atcaggagga ggggatcaag
      961 gatcccgccg tcccccttgt acgggacggg acgggtgatg tcggaggtgg tgatgcatac
     1021 ggacaggtcg cgcgcgggtt ttcccttcgc ttcgatgatc ctcgcgaggg ggggggtgcc
     1081 gacgggatcc tcgagggagc ggcggatctc gtccgcgggg tgggcgaggg gagcgaactc
     1141 ccgcattccc agcatcttgg cgtcgggggg caggaggacc tcgatcgtgc ggtccccgtg
     1201 ctcgatcttc accggcgtcc atccgccgat gtcggcgccg cggacgattt cggcgatgtc
     1261 gggccgcacg tttccctccc tacatcccga ggtacgcctg gcgcaccagg tcggacttca
     1321 gcagttcctc cccgcgcccc tcgaggacaa tccgccccgt ctggagaaca tacgcccggt
     1381 cggagatgtg gagcgcctcg aagacgttct gctcgaccag gagcacggta agccccttct
     1441 tcgagatctc gcggatggtc tcgaagatct ccgagaccag cttcggcatg atcccgaggg
     1501 acggctcgtc gagcatcagg aggctcggct gcgacatcag cccccggccg atcgccagct
     1561 gctgctgttc gccgccggag agcgtctcgg cccgttgccc cttgcgctcc gccagccgcg
     1621 ggaagagggc gagaaccgtt tcgagcgtct cctgcgtccg ctgtttcgaa tcgatggcgt
     1681 acgcccccat gagcaggttg tcaatcacgg agagtcggcc gaagagctgg cgcccctccg
     1741 gcaccatggc gatcccccgc cgtacgatgt cgtacgccgg gacggtgtgg agggcctcgc
     1801 cgttgtaaag gatctccccg gaccggggcc gcaggaggcc ggagatcgcc cgcaggacgg
     1861 tgctcttccc cgccccgttg gacccgacga ggcccaccac ctcgcctttt ttcacggaga
     1921 aggagatgtc ctggaccgcg aggaccccgc ccttgtatcc gatggaggcg ttcctgattt
     1981 caagcatgat agcgatctcc caggtaggcc tggatgactt ccgggttccg cacgacgacc
     2041 gcgggcgccc cctcgacgat cttcctcccc gagttgatga ccaccacccg atccgaaagc
     2101 tgcatgaccg cctccatcac gtgctccacg atgatcagtg taactccttt ctcgcggacc
     2161 ttccggagca gtccgatcat ttcccgcagt tccacggggg tcaggcccgc catcgcctcg
     2221 tcgagcatca gcagggtggg ctccgtgccc agcgccatcg cgatctcgag ccgcttcttg
     2281 tcggcgatcg tgatgtccgt ccccaggaag tcctttttct cgtacaaccc ggcgaaccgc
     2341 aggatctccc cggccttctc ccgcactttc ttccgatcgt aggtgcgcag gaaggttccc
     2401 gtcatgacgt tttccaggac ggtcatgtcg ctgatcacct ggacgatctg gaacgtccgg
     2461 gcgatcccca gcttgcagat cttgtgggcg ctggcggtcg agatgtcctt tccgcggaag
     2521 agaacctttc ccgcatcgga ccggtagaac cccgtgatgc agttgaagag ggtactcttc
     2581 cccgcgccgt tgggcccgat gatgctcagg atttcgcccg tgcccacggt gaaggagacg
     2641 tcctcggtcg cggtcagccc gccgaaccgc ttcgtgatcc cctcgacttc aagaacgggg
     2701 gccacggtca tttcgccctc cttctgatcg aggtgaggaa cccccagacg cccttcggtt
     2761 ggtacgcgga gatccccatt atgagcgcgc cgtagattat catgtcgatg cccgtcccct
     2821 tgtatcccag ccacacccgg gtgatctcgg acagggggag caggatcgcc gccccgatga
     2881 ccggcccgat gatcgtgccg gccccgccga gcatcgccac caggacgacc tgcacggaga
     2941 tggggaggat gaaaacgctt tccgggtcga tgtagaggat gtactgggcg tagaaggacc
     3001 ccgccatggc ggtgaggaac gcgctgatca tgatcgcgta aaggcggtag cggacgaagc
     3061 tgatccccat ccccttcgcg aggtcgtggc tctccttgat cgccttcagg tagaacccca
     3121 tccggttcgt cgagatgtac cagcagatca gcaccgaaag gagaaggaag acgaggatga
     3181 tgtagtagta gggaaccttg gaggagtgga acatgaaatt cccgagcgac tcgggaagca
     3241 gcggcaggga cagcccctcg gcggcggcga cgaacttcca gttgttgaag tggatccgga
     3301 cgatctcggc gaaggcgatg ctggcgatgg cgaagtagtg cccccgcagg ttggagcacg
     3361 ggatgccgat cgcggccccc atgagcgcgg cgaggacgcc ccccgcgacg aggccgatcc
     3421 acgggttcac gttgtagttc atcaacagaa tggtggaggt gtacgccccg atcccgaaga
     3481 aggcggcctg gccgaaggag aacatcgcgg cgtacccgcc caggatgttc cacgcgacgc
     3541 ccatcatggc gaagaggaac accatgatca tgacgtgggt
//
LOCUS       DASVJL010000513         3828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_562214, whole genome shotgun sequence.
ACCESSION   DASVJL010000513 DASVJL010000000
VERSION     DASVJL010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562214"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VF357_10565"
     CDS             <1..380
                     /locus_tag="VF357_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524638.1"
                     /translation="GKAMADLVAGVLGERGVATVRRGADPQGAEAVPVRISGVVRRFE
                     VNTRRTGDLTIVTEATVSLTITADGSGVSRPLEETVTSSASLSDLFVTPDDLHQALMT
                     TANTAAEEAARKLLEAKVVSPPS"
     gene            377..1363
                     /locus_tag="VF357_10570"
     CDS             377..1363
                     /locus_tag="VF357_10570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7524639.1"
                     /translation="MSLTIAHHLADVPLAGWEDLHGRGPRTSPFLSPRFLLPWYRALG
                     RGCDVRVARWTPGDGPDEGLLFLCRCREGGWTFLGSEQVADYLDALVVPGSAEAFWRE
                     FLERGLPALGGPLRLPGLVEGTPTLSLLPSICRETGLSCSVEEMDRAPFVSLPGSFEE
                     YLERLGTKGRHELRRKMRRAGELLPGLAFRVTRTPDELANDLPSFVELLRKSDPAKKA
                     FMDEAMAGFFREVAEGFLASGRLRLAFLATQGVDVASVFQFRTDGALLLYNSGYDPSI
                     RAANPGLVLIARSIGQAVDEGCSEYDFLRGTERYKYDLGGVDRVVYRLTVSA"
     gene            1360..2538
                     /locus_tag="VF357_10575"
     CDS             1360..2538
                     /locus_tag="VF357_10575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7524640.1"
                     /translation="MTRHLLVSYHTCPMEEPGKGLAGGMNVFLLGLLRGLSTRGIATD
                     VLTRATGETVEVSAPFPGIRIFHVPCGWKEPPSRESAFESIDLFVERSRILLRGERIE
                     PRVVSAHYWMSGVAARKLAGAPMILCYHTVEARKIPEPGAGQEPLSSIRRGNEAALAR
                     EASRVVCFSEYDLAENRRIFPELSEKGVVIPPGVDDRFRHLPPREVARGYLGLPQGEM
                     IFLLAARGDAGKNAAAAVAAFRAMRGRWKGPVTLLVAGQEGPEGGADGDVFFLGSLPH
                     GGMPILYAAADAVVCPSLYESFGLVPLEALSSGVPVVVPEGTYWGDRIRSEGGGVVYP
                     RDEPDGFSGALLALASSVELRARLSLDGAAVAAPFTWEKCTASWLALLASVSTPGSPR
                     "
     gene            complement(2465..3649)
                     /locus_tag="VF357_10580"
     CDS             complement(2465..3649)
                     /locus_tag="VF357_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX7524641.1"
                     /translation="MGKNRMGARSMGKIRVAVAGVGNCASALLQGIEFYRETPAPTDG
                     EIVPGLMNRDIGGYLPGDIGIVAAFDIDRRKVGRPVTEAIFAPPNCTKRFVERMPAGG
                     PVVRMGPVMDGVAPHMADYPDDRTFLPSSEPPCNVERVLRDSGAEILVNYLPVGSEAA
                     TRFYAEACLRTGVSLVNCIPVFIASDPAWADRFRKAGIPLVGDDIKSQLGATIVHRAL
                     ARLFSDRGIRLRRTYQLNTGGNTDFLNMLNRSRLESKRISKTEAVTSVLSHGISPDDV
                     HIGPSDYVPWQKDNKLCFLRIEGEGFGGLPIELELRLSVTDSPNSAGVGIDAIRFCRL
                     GREMGLSGPLLAPSAYFMKHPPEQHTDDDARRELEEIIADYQAWKRTQEAPARTPCTS
                     PT"
     gene            complement(3636..>3828)
                     /locus_tag="VF357_10585"
     CDS             complement(3636..>3828)
                     /locus_tag="VF357_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7524642.1"
                     /translation="CGACSAQNFVSAERLAVREIPPRCWECGRPLPIDAGDAFTGRET
                     LRETARSDPESNEGTDGKK"
BASE COUNT          530 a         1302 c         1342 g          654 t
ORIGIN      
        1 cggggaaggc gatggcggat ctggttgccg gggtcctcgg ggaacggggc gtcgcgacgg
       61 tccgccgggg cgcggacccg cagggcgccg aggcggttcc ggtgcggatc tccggggtgg
      121 tccgccggtt cgaggtgaac acccgccgta ccggcgatct gaccatagtc accgaggcga
      181 ccgtgagcct gaccatcacg gcggacggct ccggcgtttc caggcccttg gaggagaccg
      241 tcacgagcag cgcgtcgctc tcggacctct tcgtcacgcc cgacgatctt caccaggccc
      301 tgatgaccac ggccaacacc gccgcggagg aggccgcccg taaattgctg gaggccaagg
      361 tcgtctctcc gccgtcgtga gcctcaccat cgcccaccac cttgcggacg tcccgctcgc
      421 ggggtgggaa gacctgcacg ggcggggacc gcgcacctct ccgttcctct ccccccggtt
      481 cctccttccc tggtatcgcg ccctcggccg ggggtgcgac gtccgggtcg cccgctggac
      541 tccgggcgac ggccccgacg aggggctcct cttcctgtgc cgctgcaggg aggggggatg
      601 gaccttcctc ggcagcgagc aggtcgcgga ctacctcgac gccctcgtcg tacccggttc
      661 cgcggaagcc ttctggcggg aattcctcga acgggggctc cccgccctgg gcggtcctct
      721 gaggcttccg gggctcgtgg aagggacgcc caccctgtcg ctcctcccgt cgatctgccg
      781 ggagacgggt ctttcctgct ccgtcgagga gatggacagg gcccccttcg tctccctgcc
      841 gggctccttc gaggagtatc tcgaacggct gggcacgaag gggcgtcacg aattgcggcg
      901 gaagatgcgg cgcgccgggg agctcctgcc ggggctcgcc ttccgggtga cccggacgcc
      961 tgacgaactc gcgaacgatc ttccctcctt cgtggaactc ctccggaaga gcgatcccgc
     1021 gaagaaagcg ttcatggacg aggcgatggc gggctttttc cgcgaagtcg cggaggggtt
     1081 cctcgcctcc ggccgcctgc gtctcgcgtt cctcgccacg caaggggtgg acgtcgcctc
     1141 cgtgttccag ttccgcaccg acggcgcact gctcctctac aattccgggt acgatccttc
     1201 gatccgcgcg gccaaccccg ggctcgtcct cattgcgcgc tccatcgggc aggccgtcga
     1261 cgaaggctgc tcggagtacg actttctgcg ggggacggaa cggtacaagt acgacctcgg
     1321 aggggtggac cgggtggtct accgcctgac ggtttccgct tgacgcggca ccttctcgtt
     1381 tcctaccaca catgtccgat ggaggagccg gggaaaggcc tcgccggggg gatgaacgtc
     1441 tttctcctcg ggctgctccg cgggctgtcg acgcggggga tcgccaccga cgtcctcacc
     1501 cgcgccacgg gggaaaccgt cgaggtctcc gcgccgttcc ccgggatccg catctttcac
     1561 gtcccgtgcg gctggaagga gcctccgtcc cgggagagcg cgttcgaatc catcgatctc
     1621 ttcgtcgaac ggagccggat ccttctgcga ggggagcgga tcgaaccgcg cgtcgtatcc
     1681 gcgcattact ggatgtcggg cgttgccgct cgaaaacttg ccggcgcccc gatgatcctt
     1741 tgctaccaca cggtggaggc acgcaagatc ccggagcctg gggcgggaca ggagccgctc
     1801 tcctccatcc gcagagggaa tgaggcggca ctggcgcggg aagcatcccg cgtcgtatgt
     1861 ttctcggagt acgaccttgc ggagaaccgg cggatcttcc cggagttgtc ggagaagggg
     1921 gtggtcatcc cgccgggggt ggacgaccgg ttccggcacc ttcccccccg ggaggtcgcg
     1981 cggggttacc tcggactccc ccagggagag atgatcttcc tgctcgccgc ccggggagac
     2041 gccgggaaaa acgccgccgc ggccgtcgcc gcgttccgcg cgatgcgggg tcgatggaag
     2101 ggtccggtga ccctcctcgt ggcgggacag gaagggccgg aaggcggcgc cgatggggat
     2161 gtcttcttcc tcggctccct tccgcacggc ggcatgccga tactgtacgc cgcggcggac
     2221 gccgtcgtct gtccttccct ctacgaatcg ttcgggctgg tgccgctgga ggcgctctcg
     2281 tcgggcgtgc ccgtcgtcgt gccggagggg acgtactggg gggaccggat ccgttcggag
     2341 ggcggggggg tggtctaccc gcgggacgag ccggacgggt tttccggcgc gctcctcgcc
     2401 ctcgcgtcgt cggtggaatt gcgcgcccgg ctgtcgctgg acggggccgc cgtcgcggcg
     2461 ccgttcacgt gggagaagtg cacggcgtcc tggctggcgc ttcttgcgtc cgtttccacg
     2521 cctggtagtc cgcgatgatc tcctcgagct cgcgccgcgc gtcgtcgtcg gtgtgctgct
     2581 ccgggggatg tttcatgaaa taggccgacg gggcgagcag ggggcccgac agccccatct
     2641 ccctcccgag gcggcagaac cggatcgcgt cgatcccgac gcccgcgctg ttcggcgagt
     2701 cggtgacgga gaggcgcagc tccaactcga tcggcagccc cccgaaccct tccccctcga
     2761 tccgcaggaa gcagagcttg ttgtccttct gccatgggac atagtcggat ggcccgatgt
     2821 ggacgtcgtc gggggaaatg ccgtgcgaga ggacgctggt caccgcctcg gtcttggaga
     2881 tccgtttcga ctcgaggcgg ctgcgattca gcatgttcag gaaatccgtg ttcccgccgg
     2941 tgttcagctg gtacgtccgc cgcaggcgga tcccccgatc cgagaagagg cgggccagcg
     3001 cccggtggac gatggtcgcc cccagctggg acttgatgtc gtcccccacc agcgggatcc
     3061 ccgccttccg gaagcggtcc gcccacgccg gatcggaggc gatgaacacc gggatgcagt
     3121 tcacgaggct cacccccgtc cggaggcacg cctcggcata gaaacgggtc gccgcctccg
     3181 aaccgacggg caggtagttc accaggatct ccgcgccgga atcccgcagg accctttcca
     3241 cgttgcatgg aggctcggag gaggggagaa acgtccggtc gtcggggtaa tcggccatgt
     3301 ggggggccac gccgtccatg acgggtccca tgcggacgac gggcccgccg gcgggcatcc
     3361 gctcgacgaa ccgcttcgtg cagttgggtg gggcgaagat cgcctcggtg acgggccgac
     3421 cgaccttccg ccgatcgatg tcgaacgcgg ccacgatccc gatgtcgccg gggaggtagc
     3481 cgccgatgtc gcggttcatc agccccggga cgatctcgcc gtcggtgggg gcgggggttt
     3541 cccggtagaa ctcgatcccc tgcagcagcg cgctagcgca gttcccgacg ccggccaccg
     3601 cgacccggat cttccccatg cttcgcgccc ccatcctatt ttttcccatc cgtgccctcg
     3661 ttggattccg ggtcgctcct ggcggtttcg cggagggttt cgcggccagt gaaggcgtct
     3721 ccggcgtcga tgggcagcgg gcggccgcac tcccagcatc ggggggggat ctcccggacg
     3781 gccaggcgct cggcggagac gaaattctgc gcggaacagg cgccgcaa
//
LOCUS       DASVJL010000514         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_280829, whole genome shotgun sequence.
ACCESSION   DASVJL010000514 DASVJL010000000
VERSION     DASVJL010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280829"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..141
                     /locus_tag="VF357_10590"
     tRNA            46..141
                     /locus_tag="VF357_10590"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:81..83,aa:Sec,seq:tca)
     gene            complement(411..1031)
                     /locus_tag="VF357_10595"
     CDS             complement(411..1031)
                     /locus_tag="VF357_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEX7524643.1"
                     /translation="MKLLCDIPSGTEVLVHICCAPDASYGIRALRDRFDVTGFFYNPN
                     IDSREEFRRRLQATLDLREKDSFSLVVGAGGEEAWEDAARGMEGEPERGRRCEACVRL
                     RMRETARKAAELGMPAFGTVLTVSPKKDAAMVNRVGREEGDRSGIRFVEADLKKGDGY
                     LKSVRASKELGLYRQRYCGCRHSMRKDPPLGPVEQTEQGGPPNAPR"
     gene            1191..>1511
                     /locus_tag="VF357_10600"
     CDS             1191..>1511
                     /locus_tag="VF357_10600"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7524644.1"
                     /translation="MVHPADLLLGLMFNVPLLSILGIHEMGHYTAARQHRVRVSPPYF
                     IPFLPIPPLPGTMGALIRLKSPFPDRNALMDIGAAGPLAGAAIAIPVLIAGLAISEVR
                     RTTGT"
BASE COUNT          224 a          552 c          446 g          289 t
ORIGIN      
        1 gtgtattatc ggcaggaaga gcacgaaacc ttattcttaa tggacggaag cggatggggc
       61 gctggtggcc cccccggact tcaaatccgg tcgggaggcg gaaacgtctc cggtgggttc
      121 gattccctca cgcttccgcc agtcttttcc catcggaagc ccggggaatc gaagcgagaa
      181 gcccgcggcg agtcgctact ccgcaggggg gctccccgct cacatccgcc cgcccgactc
      241 cccggctgcg cccagcgacc gtgccccgcg taggtctccc agtcctctta ccgtatcctt
      301 cactccgccg agtcccctgg cttgacaatc acggggcctc cgccgtttcc atacttgcgg
      361 tctccgcccc aatacgagct tcgcgccgtt ggggtacccc cgccgtagga ctaacggggg
      421 gcattcgggg gacccccctg ctccgtttgc tccactggcc cgaggggtgg atcttttcgc
      481 atcgagtgtc ggcacccgca gtagcgctgg cgatatagcc ccagttcctt gctggcgcgg
      541 acgcttttca ggtacccgtc cccctttttc aggtccgcct cgacgaagcg gatcccggag
      601 cggtccccct cctcgcgtcc cacccggttc accatcgcgg cgtccttctt cgggctgacg
      661 gtcagcaccg tgccgaaggc gggcatcccg agttccgctg ctttcctcgc cgtctcccgc
      721 atccgcagcc ggacgcacgc ttcgcaccgg cgtccccgct caggttcgcc ctccatcccc
      781 cgggcggcat cttcccatgc ctcctcgccg ccagcgccaa ccaccaggga gaatgaatcc
      841 ttctcccgca ggtcgagggt cgcttggagc cgtctccgga attcttcccg tgaatcgatg
      901 tttgggttat aaaagaagcc ggtgacgtcg aaccggtccc gcaacgcgcg aatgccgtac
      961 gaggcgtcgg gggcgcagca gatgtgtacc agcacttccg tgcccgacgg gatgtcgcaa
     1021 aggagcttca cgagggaacc cgacccccct gtcgatgatc ttacagggga tagttaaccg
     1081 aatttttccc cgaaggggaa ggagagattc caacggtacc catcctgctg cttctggcca
     1141 ccttcgttac gacgctgttc gccggctcgt tcctcgccgg cgggaacccg ttggtacacc
     1201 ccgcggacct gctcctcggc ctcatgttca acgtcccgtt gctgtcgatc ctcgggatcc
     1261 acgagatggg gcactacacg gcggcgcggc agcaccgcgt ccgggtctcc ccgccgtatt
     1321 tcatcccctt cctcccgatc ccgccgctgc cggggacgat gggggcgctt atccgcctca
     1381 agtccccgtt ccccgaccgg aacgccctga tggacatcgg cgccgcgggt ccgctcgccg
     1441 gggcggcgat cgcgatcccc gtgctgatcg ccgggctcgc gatctcggag gtccggcgga
     1501 cgacgggcac c
//
LOCUS       DASVJL010000515         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_374746, whole genome shotgun sequence.
ACCESSION   DASVJL010000515 DASVJL010000000
VERSION     DASVJL010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374746"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            424..2016
                     /gene="peaA"
                     /locus_tag="VF357_10605"
     CDS             424..2016
                     /gene="peaA"
                     /locus_tag="VF357_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009310 - amine catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     alpha"
                     /protein_id="HEX7524645.1"
                     /translation="MQRKRRSCFVVALAFMLATAWVGSALGAPGIPVTSVLVLKKCTD
                     CHKNNGGLVSRISYLRQVPEAWEETLWRHKRIHGLSITKDEKEALLLYFSDKQGLAPA
                     EVAPYAYTLEKRDTKEKVDSQVIIDMCVRCHSYAKTALQRRAPEDWPKLANMHSGVLP
                     MWLYQLQDVLDWDDTLAACVKELQKRFPLETPEWKQWSESRPKAAEGKWVVAGYQAGK
                     GAYGGEIELKKTGDAFYSYTGTVEFESGEKQAIGGKATLYGGYAWRASGTLAGKPIRE
                     VFHISMDGSTFTGVRFDDPHFELRGVETRTFAGSSPRILSVMPKALKAGTKGATVTVI
                     GTGLSKEVSLGDGVTVRKVVSASPTKVVITVDVADKAAVGYRIAKTGGASTDKLFAVY
                     TAVDYIKVVPSPAMSRTGGLGYAVKQLVQFDAMACSKGADGAAGTGDDIEIGRVPAAW
                     KVVELASSNEDHDVDFVGEIDRNGLFTPGNEGTNPKRAMQENNMGDVWVTASYAAPGG
                     GTLYARGYLLATIPLYVQRPVQ"
     gene            2033..>2871
                     /locus_tag="VF357_10610"
     CDS             2033..>2871
                     /locus_tag="VF357_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7524646.1"
                     /translation="MGNISRMSGAEGTLLPHCSFTAEGGTYLYAAESGGLFRMDDGVR
                     DALANLSSMEAGEVPPEILSDLRRAGLLGTAGEPVSAARTAHPPLRLRSLVLMLTTSC
                     NLACRYCYEDREEGCSSPAGGGGAPREMSLESLRRGVAYLLDHDGQGRPSAAAGREAT
                     GSFAAAAGKVSVTFFGGEPLLRFPLIQAAVGEARRMASERRKEISFSITTNGTLLTRE
                     IAVFLKENGISVCLSIDGPREIHDRNRPYASGRGSYEDVARGLSVLMKNRNGFPVAAR
                     VTLG"
BASE COUNT          536 a          897 c          976 g          462 t
ORIGIN      
        1 gggggcatgg cgcctctccg cgatccagca gacttcgtag cagcggaatc ccccctccgc
       61 aagccaggcg gaggggggat gaaattttcg catcgtttcc ccggaacgac gccggtcgtc
      121 gtttctcccg gaccggcatg ggctccaaat cggggcagcc tggacattcg acgtaaatcc
      181 cgtttttgac acaccgtttc ttaattgtga tcccttgggg ctcgcgaagc ccccgccgat
      241 tcctattgcg ggtaattatc gcggcgttcg ggactgcaga cgattattat tggaaatcaa
      301 ttagttgaag ggtgcctccc tgtgaaaacc atcaaccgtg agccggtgga acgggaattg
      361 ctgcctgagg tacgagacga gatttgtttc ccccgatcat cccaccgtac gaggaggaga
      421 gcgatgcaaa ggaaaaggag atcgtgtttt gtcgttgcgc ttgcgttcat gctggcaacg
      481 gcgtgggtgg ggtcggccct cggcgcgccg gggatcccgg tcaccagcgt cctggtcctg
      541 aaaaagtgca ccgactgcca caagaacaac gggggactcg tctcccggat ctcctacctc
      601 cggcaggtgc cggaagcgtg ggaggagacg ttgtggcgcc acaagcggat ccacggcctg
      661 tccatcacca aggatgagaa ggaggcactc ctcctctact tctccgacaa gcagggattg
      721 gcccccgccg aggtggcccc atacgcctac accctcgaga agcgggacac gaaggagaag
      781 gtggacagcc aggtgatcat cgacatgtgc gtgcggtgcc acagctacgc gaagaccgct
      841 ctccagcgcc gcgccccgga agactggccg aaactcgcca acatgcattc gggcgtcctt
      901 ccgatgtggc tctaccagct ccaggacgtc ctcgactggg acgacacgct cgccgcctgc
      961 gtgaaggagc tgcagaaacg gttcccgctg gaaactccgg agtggaagca gtggagcgag
     1021 tcgcgcccga aggcggccga gggaaagtgg gtcgttgccg ggtaccaggc cgggaaaggg
     1081 gcgtacggtg gcgagatcga gttgaagaag accggagacg ccttctattc ctacaccgga
     1141 acggtggaat tcgagagcgg ggagaagcag gcgatcgggg ggaaggcaac cctctacggc
     1201 ggctacgcgt ggcgggcgtc cgggaccctg gccgggaaac cgatccgcga ggtcttccac
     1261 atctccatgg acggttcgac cttcacaggg gtccggttcg acgacccgca cttcgagctg
     1321 cgcggcgtgg aaacccgcac cttcgccggg agctccccgc ggatcctctc cgtgatgccg
     1381 aaggctctca aggccgggac gaagggcgcc accgtgacgg tcatcgggac cggcctctcg
     1441 aaagaggtga gcctcggcga cggcgtgacc gtcaggaagg tcgtttccgc atcgccgacg
     1501 aaggtggtga tcaccgtgga cgtcgccgac aaggcggccg tcgggtaccg gatcgcgaaa
     1561 accggaggcg cctcgaccga caagctcttc gcggtctaca cggccgtcga ctacatcaaa
     1621 gtggtgccgt ccccggcgat gtcgcgcacc ggtgggctgg ggtacgccgt gaagcagctc
     1681 gtccagttcg acgccatggc ctgcagcaag ggcgccgacg gcgccgcggg caccggggac
     1741 gacatcgaga tcggccgcgt cccggccgcg tggaaggtcg tggagctcgc cagctccaac
     1801 gaggaccacg acgtggattt cgtgggggag atcgaccgga acgggctctt caccccgggg
     1861 aacgaaggta caaacccgaa gcgggccatg caggaaaaca acatgggcga cgtctgggta
     1921 acggcctcct acgccgcacc cgggggcggc acgctgtacg cccgcggcta tctgctcgcc
     1981 accatccctc tgtacgtcca gaggccggtg cagtaagggg cgcgcggacc caatgggaaa
     2041 catctcgcgg atgtccggcg cggaagggac tctcctcccg cactgcagct tcacggcgga
     2101 ggggggcacg tacctctacg cggcggaaag cggcgggctg ttccggatgg acgacggggt
     2161 tcgggacgca ctggccaacc tctcctccat ggaggcagga gaagtacctc cggagatcct
     2221 gtcggatctc cggagggcgg ggcttctcgg gaccgcgggg gaacccgtgt cggcggcgcg
     2281 gaccgcgcat ccccccctgc ggctccggtc gcttgtactg atgctgacaa cctcctgcaa
     2341 cctcgcgtgc cgctactgct acgaagaccg cgaggaaggg tgctcctcac ccgcgggagg
     2401 gggaggggct ccgcgggaga tgtccctgga gtcgctccgc cgcggcgtgg catatcttct
     2461 ggatcacgat ggccagggac ggccaagtgc cgcggcaggc agggaggcca cgggttcctt
     2521 cgccgcggcc gccgggaagg tgtccgtgac ctttttcggg ggggagccgc tgctgcgctt
     2581 ccccctcatt caagcggccg tcggggaagc ccggaggatg gcgagcgagc ggaggaaaga
     2641 gatctccttc tccatcacca ccaacggcac cctcctcacc cgggagatcg ccgttttcct
     2701 gaaggagaac gggatctccg tctgcctcag catcgacggg ccccgggaga tccacgaccg
     2761 gaaccgtcct tacgcgtcgg ggagagggtc ctacgaggat gtggcgcggg gcctctccgt
     2821 tttgatgaaa aacagaaacg gctttcccgt cgcagcgcgc gtgaccctcg g
//
LOCUS       DASVJL010000516         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Deferrimicrobium sp. isolate SMAG_U3018
            k141_655309, whole genome shotgun sequence.
ACCESSION   DASVJL010000516 DASVJL010000000
VERSION     DASVJL010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959650
            Sequence Read Archive: SRR10489828
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Deferrimicrobium sp. (soil metagenome)
  ORGANISM  Candidatus Deferrimicrobium sp.
            Bacteria; Candidatus Deferrimicrobiota; Candidatus Deferrimicrobia;
            Candidatus Deferrimicrobiales; Candidatus Deferrimicrobiaceae;
            Candidatus Deferrimicrobium.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:11:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,124
            CDSs (total)                      :: 2,099
            Genes (coding)                    :: 2,095
            CDSs (with protein)               :: 2,095
            Genes (RNA)                       :: 25
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 20
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Candidatus Deferrimicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655309"
                     /isolate="SMAG_U3018"
                     /isolation_source="soil"
                     /db_xref="taxon:3060586"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..748)
                     /locus_tag="VF357_10615"
     CDS             complement(104..748)
                     /locus_tag="VF357_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HEX7524647.1"
                     /translation="MDDRIRIGLWQLNLRARKCVEALRKNGFDAHFLPDQEAVRARVL
                     AECDPAASVGFGGSMSVAGLGIFEALSGRGKKLYDHGRVPPEERAATRLAQLTCDLFL
                     TGTNAVTLEGCLVNLDMNGNRTNAMTFGPKKTVVVAGGQKIVADLPEAIRRIKTVSAP
                     RNARRLKLSTPCAATGFCEECNSPQRICRVYSIIERRRPLSDITVLVCGEPLGL"
     gene            complement(776..>1548)
                     /gene="rpe"
                     /locus_tag="VF357_10620"
     CDS             complement(776..>1548)
                     /gene="rpe"
                     /locus_tag="VF357_10620"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX7524648.1"
                     /translation="RRLLRRAPSEGVTGSPGARGRATIEPEEGNSMEYYIAPSLLSAD
                     FGQLADEVRAAEKAGADLLHVDVMDGRFVPNITIGPSVVAAIRKAATAPLDVHLMIVE
                     PERYVDDFAKAGADIITVHAEATPHLQRVVARIRELGKKAGVALNPSTSLSAVEWVLT
                     DVDMVLLMSVNPGFGGQAFLPSTLGKIELLRSQLTRAGLDVDIQVDGGIKADNVGDVA
                     ASGANVIVSGSGIFGTPDYGKTIALMKQNIRKAVGAKA"
BASE COUNT          253 a          531 c          512 g          252 t
ORIGIN      
        1 gagaaaggcg ccgccaccga tcccgaacat gccggacatg ccgccgatcg cggcgccgat
       61 cgcgaggaag ccgaggaaag aaagcggcaa gggaaggacc ctcctatagc cccaggggct
      121 cgccgcagac cagcaccgtg atgtccgaga gcggccgcct gcgctcgatg atggaataga
      181 cgcggcagat cctctgggga gagttgcatt cctcgcagaa gccggtggcg gcgcacgggg
      241 tggacagctt gaggcgcctc gcgtttcgcg gtgccgacac cgtcttgatc ctgcggatcg
      301 cctccggaag atctgcgacg atcttctggc cccccgccac caccacggtc ttcttcgggc
      361 cgaaggtcat cgcgttcgtc cggttcccgt tcatgtcgag gttcacaagg cacccttcca
      421 gcgtcaccgc gttcgtcccg gtgaggaaca ggtcgcaggt gagctgggcc aggcgcgtgg
      481 ccgccctctc ctccggggga acccgcccgt ggtcgtacag cttcttcccc cggcccgaca
      541 aggcctcgaa gatccccaat cccgcaacgg acatcgaccc cccgaagccg acggaggcgg
      601 ccgggtcgca ctccgcgagg acgcgggcgc gaacggcctc ctggtcgggc aggaaatggg
      661 cgtcgaaacc gtttttcctc aaggcctcga cgcacttccg ggcccggaga ttcagctgcc
      721 acagcccgat ccggatacgg tcgtccaatg gtcctcctcc tttccctctg ccgcctcagg
      781 ccttcgcgcc gactgccttc cggatattct gtttcatcag ggcgatcgtc ttcccgtaat
      841 cgggagtccc gaagatcccc gatccggaga cgatcacgtt cgccccgctg gcggccacat
      901 cgccgacgtt gtccgccttg atcccgccgt cgacctggat gtcgacgtcg aggcccgcgc
      961 gggtgagctg ggagcggagc agctcgatct tgccgagcgt ggacggcagg aacgcctggc
     1021 cgccgaagcc ggggttgacg ctcatgagga ggaccatgtc cacgtcggtc aggacccact
     1081 ccacggcgga gagcgacgtg gagggattga gggcgacacc cgccttcttc ccgagctcgc
     1141 ggatgcgcgc gacgacgcgc tggaggtggg gggtcgcctc ggcgtggacg gtgatgatgt
     1201 cggcccccgc cttcgcgaag tcgtcgacgt accgctccgg ttccacgatc atcaggtgga
     1261 catcgagggg tgcggtggcc gctttccgga tcgccgccac caccgacggg ccgatggtga
     1321 tgttggggac aaaacggccg tccatcacgt cgacgtggag caggtcggcg cccgccttct
     1381 ccgccgcccg gacctcgtcg gccagctggc cgaagtccgc cgaaaggagc gacggcgcga
     1441 tgtaatactc catggagttc ccctcctcgg gttcgatagt agcacgtccc cgggccccgg
     1501 gtgatccggt caccccttcc gaaggagcgc ggcgaagaag gcgtcggt
//
